LOCUS       DASHHB010000001       105394 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.001, whole genome shotgun sequence.
ACCESSION   DASHHB010000001 DASHHB010000000
VERSION     DASHHB010000001.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 105394)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 105394)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..105394
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.001"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            212..604
                     /locus_tag="U2K71_000001"
     CDS             212..604
                     /locus_tag="U2K71_000001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM7314127.1"
                     /translation="MGEKELRRSMSVFPIGTVMKLTDLSARQIRYYEEQDLVHPERSE
                     GNRRMYSLNDIDMLLEIKDYLSEGLNMAGIKRVYEMAQAKKQEAKNKKPLTDHDVRQI
                     FRDEILAQGGLSKTGHYQSQGLQRQFFD"
     gene            632..1972
                     /gene="glnA"
                     /locus_tag="U2K71_000002"
     CDS             632..1972
                     /gene="glnA"
                     /locus_tag="U2K71_000002"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HEM7314128.1"
                     /translation="MVKKQITGEEIKRIIEEENVRFLRLMFTDILGTIKNVEVPVSQI
                     DKVLENKMMFDGSSIEGFVRIEESDMYLYPDLSTWMIFPWESAHGKVARLICDIYNPD
                     GTPFAGDPRGNLKRALKDMRDLGFTSFNLGPEPEFFLFKLDEDGGITTTLNDKGGYFD
                     FAPTDLGENCRRDIVLELESLGFEVEASHHEVAPGQHEIDFKYADVVDACDNIQTFKL
                     VVKTIARKHGLHATFMPKPLFGINGSGMHCNMSLFNDEGNVFYDKDGELELSETAYHF
                     LGGLLKHARAYTAVCNPTVNSYKRLVPGYEAPVYVAWSGRNRSPLVRVPESRGLSTRL
                     ELRSVDPSANPYLAMAVLLQAGLDGIRNQITPPAAVDRNIYVMDEEEREAAHIQDLPS
                     TIHNAIKELRKDNVMIDALGQHIFSNFVEAKRLEWAAFRQTVSEWEREQYLELY"
     gene            3155..4765
                     /locus_tag="U2K71_000003"
     CDS             3155..4765
                     /locus_tag="U2K71_000003"
                     /EC_number="6.3.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthase"
                     /protein_id="HEM7314129.1"
                     /translation="MTKYIFVTGGVVSSIGKGIVAASLGRLLKNRGLKVTIQKFDPYI
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFIDINLNKYSNVTTGKIYSEVLRKE
                     RKGEYLGATVQVIPHITNEIKEKIMRAAKMTDSDVIITEVGGTVGDIESLPFLEALRQ
                     MKADVGTDNVMYIHTTLIPYLKAAGEMKTKPTQHSVKELRGLGIQPNILVVRTEQPVS
                     QSVKNKLAQFCDVEPEAVIESPDVDTLYSIPLVLQAQGMDQIVCDHLKLDTPEADMTE
                     WKELEERVLNLKKKVRIALVGKYVELPDAYISVVEALKHSGFAYNSDIEIDWIKAQEL
                     TRENVEERLKDADGILVPGGFGDRGVEGKIEAIRYARENDVPFLGICLGMQMACVEFA
                     RNVVGLEDASSAETNPDTANNIIDLMADQENIENLGGTLRLGLYPCKIKKGTKTAEAY
                     DGADVVQERHRHRYEFNNKYRQLFEEQGLVFSGVSPDNRLVEIVELPEKKFFVGCQFH
                     PELISRPNRPQKLIKAFVGASLADHETK"
     gene            5057..5926
                     /locus_tag="U2K71_000004"
     CDS             5057..5926
                     /locus_tag="U2K71_000004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010753191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphate aldolase"
                     /protein_id="HEM7314130.1"
                     /translation="MPVVSGAEFLKAARKGGYAVGGFNTNNLEWTQAILEAAEAKQAP
                     VLIQTSMGAAKYMGGYKICKDLITDLVEAMNITVPVAIHLDHGDYEAALECIELGYTS
                     VMFDGSHLPFEENLKLAKDVVEKAHAKGISVECEVGSIGGEEDGIIGNGELADIEECK
                     QMVATGIDYLACGIGNIHGQYPENWKGLAFDHLQAIADAVGPDMPLVLHGGSGIPQDQ
                     IEKAISMGISKVNVNTEFQLSFAAATRKYIEAGKDKEGKGFDPRKLLAPGKAAIVEDA
                     KSHIDWFGSANKA"
     gene            6091..7362
                     /locus_tag="U2K71_000005"
     CDS             6091..7362
                     /locus_tag="U2K71_000005"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEM7314131.1"
                     /translation="MKKIVIKGNRPLAGEVTISGAKNSAVALIPAAILADSPVILEGV
                     PDIQDVHSLIEILEIMGATVHFSNNILEIDPREVVSIPMPHGKINSLRASYYFMGTLL
                     GKFGEAVVGLPGGCYLGPRPIDLHVKGFEALGAEVTNEHGAMYLRTENKGLRGNRIFM
                     DVVSVGATINVMLAAVKAKGQTVIENAAREPEIIDVATLLNNMGAKVRGAGTDVIRIE
                     GVETLHGCRHFMIPDRIEAGTYLALAAAVGNGIKVKNVIFEHLESFIAKLQEIGVHMK
                     ISEDEIEVYPSKELKPANIMTYPYPGFATDLQQPLTALLLMTTGTSEIIDTIYAKRVN
                     HVPELARMGADITVEGNMIIVNGPNKLHGTEVVASDLRAGACLVIAGLLAEGTTTIYN
                     VDYILRGYDHIIEKLTALGASIEMIEEVGAE"
     gene            7359..8654
                     /gene="rho"
                     /locus_tag="U2K71_000006"
     CDS             7359..8654
                     /gene="rho"
                     /locus_tag="U2K71_000006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HEM7314132.1"
                     /translation="MSDYLTMAELENKTLKEVYSYAKEFKIPYYSKMNKKELSLAVIR
                     AQAEKQGFYYMEGILDIVGQDGYGFLRPINYGPSAEDIYISASQIRRFSLRNGDKVAG
                     KARPPKESERYYGLMHVESVNGKDPEEAKERPHFPALTPLYPQRQLQLETTPQRLSTR
                     MIDIFSPIGFGQRGLIVAPPKAGKTSVLKEIANGITENYPEVELIVLLIDERPEEVTD
                     LERSVKGDVVSSTFDLQPQNHTRVSELVLERAMRLVEDKRDVVILMDSITRLARAYNL
                     VVPPSGRTLSGGIDPAALYKPKKFFGAARNIEEGGSLTILATALVDTGSRMDDVIYEE
                     FKGTGNLELQLSRELSERRIFPAIDIKKSGTRKEELLMPSEELEEIWKLRKNMTGDSL
                     EYTEQLIRFLRKTKNNQQFFKEFQDVSFGKKNPTRNLKR"
     gene            8753..9016
                     /locus_tag="U2K71_000007"
     CDS             8753..9016
                     /locus_tag="U2K71_000007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016174397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L31"
                     /protein_id="HEM7314133.1"
                     /translation="MKENIHPDYHPVVFMDSTTGFKFLSGSTKTSQETVEWEDGNTYP
                     VIRVEVTSDSHPFYTGRQKFTQADGRVDRFNKKYGLKDANAAE"
     gene            9461..9910
                     /locus_tag="U2K71_000008"
     CDS             9461..9910
                     /locus_tag="U2K71_000008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM7314134.1"
                     /translation="MRFDQLIAEELIVFDPSIDSKGSLFERIGDVLKKTNKVRNVQKV
                     VKALFKREEEISTGIEDGFGIPHAKSKYVTKPALCFFHTAKLTDYSGIDEMPIEYIFA
                     IVVPEKSADMHLEILSTLSRRLMNADFRKGIKEATTPADIIRQISQE"
     gene            9967..11385
                     /locus_tag="U2K71_000009"
     CDS             9967..11385
                     /locus_tag="U2K71_000009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM7314135.1"
                     /translation="MKIVGVTACPTGIAHTYMSAEKLEVTARNLNHQAKFETQGVKTE
                     NQLSEQEIKEADAIILAVDKEIELDRFAGKKVKRVSTSRAIKEPQVVIDEALRDIGVF
                     VVSNEKEPAANEKKPTIYNHFMNGVNYMLPFVIAGGIIIALSFAFGITAADPNSADYN
                     VLAAAFSRIGGDTAFAMMVPALGAGIATSIAGKAGFAPGIVAGLLASTGGSGFLGGMI
                     GGVLAGYICDFLANKISVKKEFSAIYQLIVVPFLSILIIGLLMVFVIEQPITWVLDAL
                     TNWLNSLGNTSGLLFGLLIGIMMAADMGGPINKSTSTFSIGLMSAGVNAPIAACMAAG
                     MTPPLGIALATLLFKHKFTKEERTSGQSCWILGASYITEGAIPFAVSDPIRVIPSLMT
                     GSAVAAGISMAVGVTSMAPHGGIWVMLIPNVMNNLPMYVLAIASGTVTTAFMLGLLKK
                     TVKEKKQDKWTKNENLTNEVEA"
     gene            11385..12245
                     /locus_tag="U2K71_000010"
     CDS             11385..12245
                     /locus_tag="U2K71_000010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketose-bisphosphate aldolase"
                     /protein_id="HEM7314136.1"
                     /translation="MLYTMKELLKVAKENQFAVPAFNICSYDMMKAIIEEAERLEAPV
                     ILEIHPDEIAYLSDEFVAAVRAYAYTSKLPIVIHLDHGGTIEDVLRAIRNGYSSVMID
                     ASLKSFDDNVQITKKVVALAHAVNVSVEAELGTIGNNGSAEGGAETIIYTDPDQAKIF
                     VKETGIDTLAVAIGTAHGLYPKNRTPKLNIDLLKELNQQIDLPFVLHGGSGNPDAEVS
                     EAVKYGVAKVNLSSDLKSVFFNALREILSENPSMYEPNAIYPAANKKVQEVVKHKMEI
                     LNTVGKASLY"
     gene            12419..13192
                     /locus_tag="U2K71_000011"
     CDS             12419..13192
                     /locus_tag="U2K71_000011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM7314137.1"
                     /translation="MLNEVENRQQEIVNYLKNNQFARVTDLIELVNYSEATIKRDLVF
                     LEKSGLIRRVRGGAMLIDNRKIDVPYLMKITKLEEETEKRKIADTASSLIRDDMVLFL
                     DSSTTSLHLVRNLARFDGLQIITNGIVTAAMLSEYTTAKVSVLGGAIVPKRATINGSK
                     AYNDALSYYADLAIMSCRGLDFSQGVTETHEGEAMVKKAFRKQSDRLAVLVTQQKIAH
                     KFMHQAIAFHEIDYLVTSKELTKEQYAKVKEHHIECLYG"
     gene            13339..13704
                     /locus_tag="U2K71_000012"
     CDS             13339..13704
                     /locus_tag="U2K71_000012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2512 family protein"
                     /protein_id="HEM7314138.1"
                     /translation="MKHVKALVVKAIMIWAILWVVLTGLYGVSFMDSTIVGVIIVVMI
                     YVLGDLLILRKVGNIAATIADAGSAAVILWLYLYFMNDTVDIWMKVLIPALLIGVAEW
                     FFHKWLLSQGIVPDERKME"
     gene            complement(13852..15060)
                     /locus_tag="U2K71_000013"
     CDS             complement(13852..15060)
                     /locus_tag="U2K71_000013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7314139.1"
                     /translation="MEKKLFNKYFIGITLINFIVYLIYYLLMVIMAVIAQDTLHASLS
                     QAGFASGIYIIGTLLARLFMGKQLELFGRKFTLRGGAIFYLLSTLAYLYTPTIGALYL
                     VRFLNGFGYGTVSTATNAIVTAYIPASKKGEGINYYGLSTSLAAAVGPFLGMILLNMT
                     DFRFIIWFSIVLVFFVTIACLVFPVKNIPLSEEQKISLRSWNINSFIEKKALFITGIA
                     FLMGLSYSSVLSFLSSYTKVIHLVAAGSFFFVVYALVITFTRPLTGRIFDVKGEQYVM
                     YPSYLFLTAGLFLLSVTTNSVTLLISGALVGLGYGTFMSNGQAVCLKIVREHRISIAL
                     STYFIGLDLGLGVGPYLMGMLKSLTSFRGLYVIAGVIPLICTALYLVNGRKHSHEAEG
                     ELIESLKNEI"
     gene            complement(15079..15357)
                     /locus_tag="U2K71_000014"
     CDS             complement(15079..15357)
                     /locus_tag="U2K71_000014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314140.1"
                     /translation="MNYWMNTIINRLETAYQTRFDMKASLVFLNDAYQNSIELIKAVD
                     ENPTNECEEFLNLFMSTRDLFIRQLVDRYPSNYHDVEVQIQKLKAYSA"
     gene            15511..16206
                     /locus_tag="U2K71_000015"
     CDS             15511..16206
                     /locus_tag="U2K71_000015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM7314141.1"
                     /translation="MIFLDESLDGGASLDSRVLKEYLDKNDFPIIRKGYHKYLTFEGM
                     EDSHTYILKEGTVKASVISNDGREFNLRYIKDLEIVSLLKDEYSQFIDSPYNIRVESE
                     YAEFYRINRVRFWQNINQDPKLQHYVKEYYRYRLLYSMKKMQQMLLNGKLGAVCTQLY
                     ELYDLFGVRTEEGMLIDFSITNEEIAKFCGISTASSVNRILKQLKKDGIITVKDHHIV
                     ILNMDLLEDNIIF"
     gene            complement(16648..17445)
                     /locus_tag="U2K71_000016"
     CDS             complement(16648..17445)
                     /locus_tag="U2K71_000016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HEM7314142.1"
                     /translation="MMKKFFLFFLLLVSVGWPVATHADTATVNDEAGLFTTDQIQSLE
                     QQAEKLNEKIKGRVLIVTTTSNSEEPRDFADNYLRDAVGNDQNGSVLLLDMGQREIYI
                     STSGNMIDYLTDSRIDSILDDVYDQMTNAAYYNAAKAYLSKSATYVEDGVPGGHYRVD
                     EETGKITRYKVLTTVEIIIAVVLALILSLVFYFVTVSRYQLKSGIYKYPFREKSSIKL
                     TEKTDRLTNSFVTTRRIPKSPPPGSGGGGSTTHSSGGGTFGGGGRSF"
     gene            complement(17442..18626)
                     /locus_tag="U2K71_000017"
     CDS             complement(17442..18626)
                     /locus_tag="U2K71_000017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEM7314143.1"
                     /translation="MDVLTHKCPNCGGPLTFDPKDQKFHCEYCLNIYTEEEVSQYEQK
                     QKEARMAGEESKEEPSPTEDFTFTAQEQLADMNEAERKAVDEAGGFSDTADESADNTA
                     AIDLFLCPNCGAEIVTDATTAATYCYYCHNPVVLSGRLSGEFLPNKVLPFAVEKEEAI
                     NRFLAWTKKKWFVPKAFFNKDQIDKLTGVYFPYWATDAEVDGAMQANGTVIRIWRIGD
                     IEYTETKQFAVSRAGKLSFKELVKNALSKNTQQKMVEGVQPFPLDKAIDFKSQYLAGF
                     QAEKRDIEYEAIKSQIQSELKDYSEKLLKDTANGYTTLTGVRTSASITNEVNNYVLLP
                     VWLVTYRSNDSSKKVYYYAMNGQTGKVSGVLPISQKKLGLTALGIFAGLAILFLIGGY
                     LI"
     gene            complement(18651..19898)
                     /locus_tag="U2K71_000018"
     CDS             complement(18651..19898)
                     /locus_tag="U2K71_000018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEM7314144.1"
                     /translation="MGLIKAATSMVGGGLADQWLEVIEPDNMSDTTVMTKGVKVRKDD
                     KRGSNRKGTEDVLTDGTVVHVYPNMMMLLVDGGKIIDYTAEEGYYTIKNDAAPSMFNG
                     TLKEAIAETFDRFKFGGVTPQKQQVFYINLQEIKGIKFGTSAPLNYFDNFYNAELFLR
                     AHGTYSIHVVDPILFYTNAIPKNKTQVEINDINEQYLAEFLTALQSAINQMSADGQRI
                     SYVPSKSLELSKYMDTALDDSWRELRGMEIVSVAVASISYTDDSVKLINMRNEGAMLG
                     DPSVREGYVQGSIARGMEAAGKNEAGAMTGFMGVGMGMNANGSYLSQAAQNNQEQIKQ
                     QAEKQNQQTAQGNADTWTCPVCGTENSGKFCSNCGAAKPVENVQPKLQMRCSECNEIV
                     DLSNGIPKFCPNCGKPFKGIPVN"
     gene            20113..21858
                     /gene="dnaX"
                     /locus_tag="U2K71_000019"
     CDS             20113..21858
                     /gene="dnaX"
                     /locus_tag="U2K71_000019"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HEM7314145.1"
                     /translation="MAYQALYRVWRSQRFEDIVGQKAVTQTLKNAIVSHKTSHAYLFT
                     GPRGTGKTSAAKIFAKAINCPNSKDGEPCNECEMCRSITAGTQEDVIEIDAASNNGVE
                     EIRFIRDRANYAPTKAAYKVYIIDEVHMLSTGAFNALLKTLEEPKESVIFILATTEPH
                     KIPATIISRTQRFDFKRINTADIVEHLEFILKESTISYEPAALNVIARSAEGGMRDAL
                     SILDQAISFSEGTITLDDAMQVTGSLTDEMMDSYLSACVKKEVPEALETLNQILAAGK
                     ESRRFLEDMLQYCRDLLMYQQAPNLVEEQSSTIRSTFTELAQTVSADQLFSMIKILSD
                     TQNEIRFTNSAAIYLEVATVKLAQLPSASVPSADVAFADDQIVKQLQQEIKELRNEIK
                     ELRSNTGIENTKVQPVQQTNHKKQTSTYRVPTERVYKVLREATRQHLNQVKEVWEDLL
                     MSLSVTQRAMLKASEPVAAGPTGLVIAFDYEIVCQRAANDEELQLSVHNNLSRMIKDY
                     APDPVYITRESWPELRRNYLTQAQEEGTENSFASEDSEGEIELLPQEQEVPEETLVVK
                     AEELFGSEAVTVVDD"
     gene            21878..22192
                     /locus_tag="U2K71_000020"
     CDS             21878..22192
                     /locus_tag="U2K71_000020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HEM7314146.1"
                     /translation="MMRGMGNMQGMMKQVQKMQKEMAEAQELLNKTEFTGKATNELVK
                     AVFTGDRRMKDIQINEEVVDPDDIEMLQDLVVMAVNDAIAQIDVETEKKLGKYAKGLP
                     GF"
     gene            22217..22813
                     /gene="recR"
                     /locus_tag="U2K71_000021"
     CDS             22217..22813
                     /gene="recR"
                     /locus_tag="U2K71_000021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination protein RecR"
                     /protein_id="HEM7314147.1"
                     /translation="MQYPEPIAKLIDSYMKLPGIGQKTATRLAFYTIDMKDEVVNEFA
                     KSLLSVKRDLHFCSICGNITEEDPCEICRNPSRDRSVILVVEEPKDVMAMEKMREYHG
                     LYHVLHGVLSPMEGTGPEDINISSLLQRLHDADVKEVIIATNATTEGEATAMYLSRLI
                     KPAGIKVTRLAHGLSVGSDIEYADEVTLLKAVEGRREL"
     gene            22877..23521
                     /locus_tag="U2K71_000022"
     CDS             22877..23521
                     /locus_tag="U2K71_000022"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HEM7314148.1"
                     /translation="MNGLFITIEGPDGAGKTSILNELYPLLKEVAKTEIIQTREPGGI
                     PIAEEIRAVILDPKNDRMDERTEALLYAAARRQHLVEKVLPALAKGKIVLCDRFVDSS
                     LAYQGAGRRIGVTEIARLNEFATEGTTPDFTLYLDVDSDTGLRRIKKNRQNQIDRLDS
                     EGLEFHQRVRHAYLKLAEENPERIHKVDARKSFEEVLQTSYHTIIEQYPQFFEN"
     gene            23536..23865
                     /locus_tag="U2K71_000023"
     CDS             23536..23865
                     /locus_tag="U2K71_000023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HEM7314149.1"
                     /translation="MKIILAIIQDKDSNRLSNELIDANIRATKLSSTGGFLKAGNSTF
                     IVGIEDDRVEEALEIIKKTCESRKQFVSTPVTLDISMDGGVPYPVEVEVGGATVFVLP
                     VEGFHQF"
     gene            23879..24817
                     /gene="holB"
                     /locus_tag="U2K71_000024"
     CDS             23879..24817
                     /gene="holB"
                     /locus_tag="U2K71_000024"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HEM7314150.1"
                     /translation="MNQEINLAQMQPIVYQQLQRSFEHGRIAHAYLFEGEKGTGKHEV
                     GIWLAQHLFCTQMKDNLPCGKCNNCQRIEKKEHPDVLVIEPEGQTIKVDQIRRLQTEF
                     SRSGYESRKKVFLIKEAEKMNSSAANSLLKFLEEPPGDFLAVLETDAVGRILPTIQSR
                     CQVLHFQELSKEALIHRLQEEQIPLEKAKLLAFLTNSLGKAVEISQDEWFNDAKDLIY
                     QWFVYLQKQDTQAFIYVQKKLVKSFKEKTQQFTGLSVLMFYYQEAQRQAVANGEYGKV
                     KQINQIIERILIAEQKLRSNVSFQAVAEQFVLHTIF"
     gene            24853..25677
                     /locus_tag="U2K71_000025"
     CDS             24853..25677
                     /locus_tag="U2K71_000025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stage 0 sporulation family protein"
                     /protein_id="HEM7314151.1"
                     /translation="MVEVVGVRYRDAGHIYYFAPGDSEYVYSDKVLVESQQSLHIATV
                     VLPKKEIDPNDLPEAIRPILHKASQAELEKDKKNKKDAYDALKIAKEKVQLRELEMKM
                     VKAEYTFDRNKLIFSFTADGRIDFRELVKDLAAVFRTRIELRQIGVRDEAKIIGGIGP
                     CGRPLCCSSFLGDFVPVSIKMAKDQSLSLNPTKISGLCGRLMCCLQYESEEYETAKKE
                     LPDFGKVIATPDGKGKVVGLNLLSRIVSIRLTGRENPVEYEWDELKEFIQVGEMNG"
     gene            25670..26017
                     /locus_tag="U2K71_000026"
     CDS             25670..26017
                     /locus_tag="U2K71_000026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication initiation control protein YabA"
                     /protein_id="HEM7314152.1"
                     /translation="MDKRSLYDGLNQLESELRNTLTELVEMKHALHEIVEKNTTLEME
                     NQRLREHLRELNQVAQAPAENVKQELSKSRMNLEKIYEEGFHVCYDLYGSRRDNDEPC
                     AFCLEVIYRESGR"
     gene            26086..26958
                     /gene="rsmI"
                     /locus_tag="U2K71_000027"
     CDS             26086..26958
                     /gene="rsmI"
                     /locus_tag="U2K71_000027"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HEM7314153.1"
                     /translation="MYNQKSFKGNPVYGDLYLVPTPIGNLEDMSYRSVRMLQESDLIA
                     SEDTRNTQKLLNHFEIHTPQKSLHEHNYKERVPQLIALLKEGKTIAQVSDAGMPSISD
                     PGHELVLACIKEGISVISIPGPTAGMTALIASGLVPQPFLFYGFLPRKKKEQKETLKK
                     LKEQTVTLIFYESPYRITATLTNFLEIFGNRQVVLCRELTKVHEEYLRGSTEELLAYI
                     EEHPVKGECCLLVEGNLFEMPVTEVDEELGTLKEQVEAKIASGEKPNAAIKAVALKNG
                     LKKQEVYRQYHELD"
     gene            27067..28188
                     /gene="dinB"
                     /locus_tag="U2K71_000028"
     CDS             27067..28188
                     /gene="dinB"
                     /locus_tag="U2K71_000028"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HEM7314154.1"
                     /translation="MMELQFPLHNDTSRKIIHIDMDAFFASVEERDHPEFRGHPLVIA
                     RHPSDTGGRGVVTTANYEARKFGIHSAMSAQKAYELCPQAIFKPGDHHKYSDISKQVR
                     EVFYRYTDIVEPVSIDEAYLDVTENKINCKSAIKIARMIQADIWESVQLTCSAGVSYN
                     KFLAKLASDYQKPRGITVVAPQDAVPFLKALPIEKFHGVGKKTVPRMHELGIYTGKDL
                     YECTEMMLIRNFGKMGYSLYRKVRGIHDSPVNVTRERKSVGKEHTYGQPLQTEEAVLT
                     QLRQLAEKVEEALRRVQKHGKTVVLKVRYTDYSTVTKRVTLPEYIYKKEALFYQASLI
                     WEEILGVEKGIRLLGITLTNLDPMTYENIVLPLWENQEI"
     gene            complement(28242..28844)
                     /gene="nrdG"
                     /locus_tag="U2K71_000029"
     CDS             complement(28242..28844)
                     /gene="nrdG"
                     /locus_tag="U2K71_000029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase
                     activating protein"
                     /protein_id="HEM7314155.1"
                     /translation="MRNPKPKEWLAKDYSQNYIADYKPFNFVDGEGVRNSLYVSGCLF
                     ACEGCFNKAAQNFRYGKPFTPELEEQIIADLRNDYVQGLTLLGGEPFLNTDVCLQVVR
                     RIRKEFGTTKDIWSWTGYTFDELLQDSEDKLELLSQIDILVDGRFELSKRDLKLQFRG
                     SSNQRIIDVQKSLESNQVVIWEKCTDATETYEQIKKQDLI"
     gene            complement(29159..31348)
                     /gene="nrdD"
                     /locus_tag="U2K71_000030"
     CDS             complement(29159..31348)
                     /gene="nrdD"
                     /locus_tag="U2K71_000030"
                     /EC_number="1.17.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anaerobic ribonucleoside-triphosphate reductase"
                     /protein_id="HEM7314156.1"
                     /translation="MKVNYEEKTVVTQQPETSQMKVIKRDGRHVDFDEQKINEALIKA
                     EKKIHGSLSPLTYEKIQMIADLVVQEIKSRFAENVKIYEIQNIVEHILLEKNEYELAE
                     EYINYRTKRDFARSKATDINFSIEKLINKDQTVVNENANKDSNVFNTQRDLTAGIVGK
                     SIGLKMLPPHIANAHQKGDIHYHDLDYHPYTPMTNCCLIDFKGMLNNGFKIGNAEVES
                     PKSIQTATAQISQIIANVASSQYGGCSADRTDELLAPFAELNYKKHLKDAEEWIDSPE
                     RQKEYAKAKTKKDIFDAMQSLEYEINTLFTSNGQTPFTSLGFGLGENWFEREIQKAIL
                     QIRINGLGSEKRTAIFPKLIFTLKKGVNLNPEDPNYDIKQLALECATKRMYPDILNYD
                     KIVELTGSFKVPMGCRSFLQGWKDENGQEVNVGRMNLGVVTLNLPRIAIESKGDQNKF
                     WQLLSDRLEIMKDALLYRVERCKEAIPANAPILYMYGAFGKRLSRTDSVNELFKNRRA
                     TVSLGYIGLYEVASAFFGGEWETNPEAKAFTLDIVKELKANADAWGDEYGYHFSVYST
                     PSESLTDRFCRLDTEKFGIIENITDKEYYTNSFHYDVRKNPTPFEKLEFEKDYPKYCS
                     GGFIHYCEYPMLQQNPKALEAVWDFAYDKIGYLGTNTPIDHCYQCGFEGDFKPTERGF
                     ECPECGNHDPKTCDVVKRTCGYLGNPQARPMVHGRHKEISSRVKHMK"
     gene            31647..32441
                     /locus_tag="U2K71_000031"
     CDS             31647..32441
                     /locus_tag="U2K71_000031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314157.1"
                     /translation="MKNERGGEELEKMIEVKHLSKSYGSKLSPVKALDNISFTVEEGE
                     FVGIMGPSGAGKTTLMNILSTINLPTMGSVSIQGKEITKMKNSELSDFRRKKLGFIFQ
                     EFNLIDTLNAKDNILLPLAVERMTKEEMEKRVRHVAQLLNIEELLKRYPDELSVGQRQ
                     RIAAARALVVQPQVIFADEPTGSLDSKSATELLNYLKTMNQKEKATILLVTHDPYTAS
                     YCDRILFIKDGVIFSEVVRRGTRREFFEKVIDMQATIGGGGKMNAV"
     gene            32431..34473
                     /locus_tag="U2K71_000032"
     CDS             32431..34473
                     /locus_tag="U2K71_000032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314158.1"
                     /translation="MLYKLAVKSFFRQSRGYLVYFFSLTLSVMIYYSFSAMTYDQSLV
                     RRASQDTSIDGGLSFGGFIITVVLLFFVFSANRFFLNRRQKEIGIYQLFGMNKLQISG
                     IYVLEIMIIGLFACISGILLGIIFSKLFSMILVRMMDMDLTSPFFISIPSVVDTLFAF
                     FIILLAVSVYSIWKIWRYPMIRSFGEKEQADSSTMRFRTRHRLLAVIGLLLLTSGYFG
                     ASHFREITSWMISYHYLDTLVFFFPFLIFFVCVVGTYLFFCYTFRLFLIILSKWKIYY
                     YGINFLIMGNTQVHLLKSWRTNSLITVILGISLMTIGGTASISTILSYQEKVSSPMDY
                     QFDVETEKKIKPILAEEGQKITKEITLHYKVIGSIYSQNLEEPMFQTANLITETQYQA
                     FRKVNPDLPNISLKSPGHTVLLDQMQTLFRNISLYGSSIGLPDNQELKIQQMLPSVLG
                     DETMVYLSPVLIVDDTVFEKAKGVDYQVVNVDVSGGNNEKIDERLSNDLSIKWLNAVY
                     YSYDIVNGSLQGEISTKKLPDDQMKEAQFSQESSRLNFSSSYSKLRAVNRRIGINIFV
                     TLFVGMIVIIATGSILTVRQLAEAEEEKENYHLLTKLGIPQRRIRRMIFEQNALTFFP
                     PMILGITHAVFAINVFTQYIKTADYWLAYLVSGLLILIYLLLYVITSQLYCRIIEE"
     gene            34754..34903
                     /locus_tag="U2K71_000033"
     CDS             34754..34903
                     /locus_tag="U2K71_000033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="teichoic acid D-Ala incorporation-associated
                     protein DltX"
                     /protein_id="HEM7314159.1"
                     /translation="MNKWRSNPKLNYWGKFIIRTIFYSAVLLFLIYLYHYKNIQGGTF
                     IYNEF"
     gene            34914..36428
                     /gene="dltA"
                     /locus_tag="U2K71_000034"
     CDS             34914..36428
                     /gene="dltA"
                     /locus_tag="U2K71_000034"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltA"
                     /protein_id="HEM7314160.1"
                     /translation="MEIKTIIEAIDDWGINEPDRVAYQADETHTFGELKQASDALAYY
                     LEKKVEGDGPIVVFGNLEFEMIVSFLGVVKSGHAYLPIEEHTPKERILSIFRVAKPSM
                     VISIGDWIEDIPSVPVITKKEFQEIKKIPVGFYPRNSVKGTENFYIIFTSGTTGEPKG
                     VQISHDNLVSFVQWTMEDFGIKPGMHFLAQAPFSFDLSVFSIYPALVSGGMLKPLNKA
                     VVQDFRQLFATLPALKLNVWVSTPSFMDICLMEPTFNGENVPELDMFLFCGEELTKKT
                     AESLLDRFPNARIYNTYGPTEATVAISSIQIDQHVLDSYDRLPIGYVKKDTKVSIVQE
                     GKPVSKGETGEIIIAGPSVSKGYLNNPEKTAAAFFCQNDLASYHTGDAGRLDEYGLLF
                     YEGRMDQQVKLHGYRIELGDIEHYLLQDNRIKQAVVVPKYQGTKVQQLVAFVVLENKT
                     KQPDFQLTKSIKEQLLEVVMDYMIPQKFHYVDLLPQTVNGKIDRKKLTAEVNPE"
     gene            36425..37630
                     /gene="dltB"
                     /locus_tag="U2K71_000035"
     CDS             36425..37630
                     /gene="dltB"
                     /locus_tag="U2K71_000035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltB"
                     /protein_id="HEM7314161.1"
                     /translation="MMLPHMIPYAEPFYFFLLAIALVPIVLSLLIWEKRLPVYQSAVT
                     LFFLFVSFGGNYWQQGAALIGYVLWQTILVWGYFVYRQRKNQTGWFFAAVILAIIPLF
                     LTKVTPFFEHGKASLLGFLGISYLTFKSVQMIMEIRDGMIKTYKPFRYIQFLLFFPTI
                     SSGPIDRYRRFEKDQLHPPVRDKYVDLLGKGVQNIFVGFLYKFIIGYYFGQVLLPVVG
                     RIALQHGGISWALVGYMYVYSMYLFFDFAGYSLFAVGTSYMMGYQTPINFNKPFLSWN
                     IKEFWNRWHMTLSFWFRDYVYMRLMFFMMKKKFFKSKIVMSNIGYFALFLLMGIWHGL
                     TWYYIVYGLYHACLICLTDAWIRFKKKHKEQLPSNKFTHGLAVFLTFQAVCFSFLIFS
                     GFLDKLFFS"
     gene            37675..37908
                     /gene="dltC"
                     /locus_tag="U2K71_000036"
     CDS             37675..37908
                     /gene="dltC"
                     /locus_tag="U2K71_000036"
                     /EC_number="6.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--poly(phosphoribitol) ligase subunit
                     DltC"
                     /protein_id="HEM7314162.1"
                     /translation="MEEQILSILEEITGTDEVRKNRDIDLFDEGLLDSLASVQLLVEL
                     DGELDIQVPVSEFEREDWCTPNKIIQQATALKG"
     gene            37911..39179
                     /gene="dltD"
                     /locus_tag="U2K71_000037"
     CDS             37911..39179
                     /gene="dltD"
                     /locus_tag="U2K71_000037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-lipoteichoic acid biosynthesis protein
                     DltD"
                     /protein_id="HEM7314163.1"
                     /translation="MKKKLFAIFGPVVLAVGLLFVFFTSSYQVDISNPETIRRASSSM
                     SVNVLKGDAIKNYALSEKQYIPFFGSSELSRISPFHPSVLAEKYQRNYRPFLLGAPGT
                     QSLSQYMMMRSAGDAMKNKKVVFIISPQWFVKNGVKTDYFNTYYSELQTYDWLFSMKK
                     VTPADRYLARRLLTFSKVKENDTLTAILQTIKKGKLPLPESLNQLRSQWNMLKREDEV
                     FSNIGLKDRQQKIDHESKRLPKQYQETELSILANQIGERETTNNPFGLKNDFYTHRIR
                     AHEPELKQSQKNWDYRFSPEFSDFQLVLDQLAKNHNEVLFIIPPVNEKWSDYTGLSQE
                     MLQGFAKKIKFQLNSQGFNRIADFVNQAGTNYFMEDTIHLGWKGWLAADQQIRPFLEE
                     NHITASKYHLDDAFFSKSWQHQIPDKLQLK"
     gene            39320..40402
                     /locus_tag="U2K71_000038"
     CDS             39320..40402
                     /locus_tag="U2K71_000038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M42 family metallopeptidase"
                     /protein_id="HEM7314164.1"
                     /translation="MDTKEEQLLIDLTSAKGVPGNEEEVREVFREYAKPFADDIFYDG
                     LGSVIAKHGAGGGPKVFISGHMDEVGFMVTKITEKGFLEFQTLGGWWGQVMLAQQVEI
                     KTREGKIVHGVIGSKPPHVLTPQVRNKPYEIKDMFIDIGASSQEEAKEWGIRPGDMVT
                     PYIEYKRMNGSKYLLAKAWDNRIGTAVSLRVLENLSKEAHPNVLFAGSDVQEEVGLRG
                     ARTSTHLVNPDIAFALDTGTAGDTPGMTPKEADSILGKGPQILIFDASMIPHKKLLNF
                     VIDVAEEMDIPFQYTVISGGGTDAGQMHLTRDGVPSVAITVPVRYLHSHTSVIHEDDY
                     LNTVKLVTELVKRLDSETVEKIRSYE"
     gene            40402..41205
                     /locus_tag="U2K71_000039"
     CDS             40402..41205
                     /locus_tag="U2K71_000039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEM7314165.1"
                     /translation="MNNRIPISEYRKKARVSLENQWGINTWLVFLSIIFTSVIQNFFD
                     GFSPFPEESMQQHLLSFLVDNLLLFAFNYALFYIALVVVRGGRPKSNLLFSVFQRKYF
                     GSILGINLINSVINWLLNSLIFLPMFLISGVSTYMQLLFSSNAVPTGVMPEDFLDISF
                     VLSMIGMIFVSALIMSIVGGLFQFAAWTKFDYPDLSVIQCLKYGWFLLKDRIGTYILL
                     QLSFIGWYLLGAIALFIGLLWVIVYVNVSVAEFYEQARIEKGGPDAYFT"
     gene            41275..42015
                     /locus_tag="U2K71_000040"
     CDS             41275..42015
                     /locus_tag="U2K71_000040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM7314166.1"
                     /translation="MKLTVLGCLGAYPYKNQGTTGYLLQSDGFNLLIDAGSATLIKLQ
                     EHLDPLDLDAVILSHYHHDHIADLGVLQYYWQLHPKRNPETLLSIYGHTKDSIHFEEL
                     TLENVSKGHPYFEAEELKIGPFLITFMETIHPVVCYAMRIVEETTGKVFVFTGDSGYL
                     ESFADFAKDADLFLADTYLFEGNEHHHAHFTSKEAGELAKSANVKKLVLTHLPQFGDL
                     EQLKNEAQKAAGKGIDIELAAVDKVFNI"
     gene            42026..43030
                     /locus_tag="U2K71_000041"
     CDS             42026..43030
                     /locus_tag="U2K71_000041"
                     /EC_number="6.3.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase"
                     /protein_id="HEM7314167.1"
                     /translation="MIFVPNENHDPRINLAIEQYLLQEMPLDEPILLFYINEPSIIIG
                     RNQNTLEEINRDYVEENGIHVVRRLSGGGAVYHDFGNLNFSFIMPDDGDSFRDFAKVT
                     QPIIQALHELGVAGAELKGRNDLVIDGMKFSGNAMYATNGRMFAHGTIMFDSDINEVV
                     NALKVKKDKIESKGIKSIRSRVTNIKPFLPEEKQKMTTEEFRQEILLKIFGVESVEEV
                     KTYELTDEDWKKINRISEEYYRNWDWNYGKSPDFNFSRQKRFSIGSIEVHLNVSEGMI
                     KEAKIFGDFFGLGDISDVEKKLVGQKYDKASLSKIVSQIDVKKYFGAIEPEELLTLLY
                     "
     gene            complement(43075..44919)
                     /locus_tag="U2K71_000042"
     CDS             complement(43075..44919)
                     /locus_tag="U2K71_000042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314168.1"
                     /translation="MSFFNNIYNKKESFYLRIKWSIIIILSLLFLTGIAYFFYFIFSP
                     ADSNQMKEIKDSWVFYTKDDPDFKFDSKYTRTIPTVDKDETFIMETTLEKPLDEANLL
                     IKGNHQWITVYLGEKILYQRKDYQEESNPGLSLAVIDLPANYIGKKLTLTVSSPYQNY
                     AGLPPKVYLGTTGPLISFIYSQSVPQVLTMIIAVFIAIGTFFYTIYLMYKQKRLEYSL
                     ILLSCFALALGFEAVSEDILSGILFEPFVHSFLSHLFIILTSAFIICYYWSRMFYYKK
                     WYGIFCTFQLLIFIGVLLYATFTPAELPELMPIANIASVISTLVTSLACIGEAYKNNR
                     FYVACTPWIVLVAIIHCLIYIQTALGIYQTTINWSTILYIIILIVIISYNLIDHILNT
                     DKDRRQVDFLKIKNDLLEQHYEQLRQHIQEVNTLRLEFVQEMENLQDLMHTDQTKAKN
                     YVEKILEEAKNFEMLCSFCNHQVTNLILARYQKLAAKRNIQITFQADLPEELPINDDD
                     LAQLLIHALEHSFRETHAIENPLYRKIYLSIHEQEEHFVICCEHSAHYDTNLFSRGIT
                     EELDDQERFDLMMMKDVADRYTGTLTQEKDTFIDRITVQLSRNYPNPV"
     gene            45131..45649
                     /locus_tag="U2K71_000043"
     CDS             45131..45649
                     /locus_tag="U2K71_000043"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM7314169.1"
                     /translation="MPDYKKITFISSITLIIFLILILLVVLVPRIFGLTPYIIEDKEM
                     SPYYAQGNLIYVRPKNTNEILVGDIITYYENSGKKISTRRVIAVENNHEDFYTKADTK
                     TYIEPGVVKKRNIIGSPIFQIPYIGLVLSKTAFAWIQGLFFTIAFCLTGITAWNTFVE
                     LKKKRRRTQKFL"
     gene            45777..46460
                     /locus_tag="U2K71_000044"
     CDS             45777..46460
                     /locus_tag="U2K71_000044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HEM7314170.1"
                     /translation="MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPV
                     IPHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTIGENAHVTTIDRFDVMIEKA
                     KKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLRLLRKGG
                     VLMVDDIFQGGTILLPDEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILM
                     TKESETIEL"
     gene            46769..46842
                     /locus_tag="U2K71_000045"
     tRNA            46769..46842
                     /locus_tag="U2K71_000045"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:46803..46805,aa:Arg,seq:tct)
     gene            47001..47360
                     /locus_tag="U2K71_000046"
     CDS             47001..47360
                     /locus_tag="U2K71_000046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1304 family protein"
                     /protein_id="HEM7314171.1"
                     /translation="MTILSRILVTLVAIEFFYIMYIETVRTDSDTTSRVFKMSKEELS
                     RKSVQTLFKNQGVYNGLLGVGLLYGAYLSSASKEITSMLLISIFFVALYGSLTSDRWI
                     LLKQGGLALLALLSIFL"
     gene            47483..49114
                     /locus_tag="U2K71_000047"
     CDS             47483..49114
                     /locus_tag="U2K71_000047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HEM7314172.1"
                     /translation="MFKNYVSSLKKEFSGYDLSRFQKDLLAGITVAAVALPLALAFGV
                     SSGADAAAGLITAIIAGIVIGGLSGGFYQISGPTGAMAAILMSIAATKGMQGVLLATF
                     LAGLFLLAAGLFRLGTLTSFIPAPVITGFTSGIAIIIALGQIDNLFGVHSEGTNVIEK
                     LSSYQTIGFPINTASLIMGGLVILGMFIYPKKWAKRMPSSLLAIILATAVMLLFHLPV
                     ATVGKIPQTLISSNRLNMGDFSFSALQQVAVPAISIALLGMIESLLCGASAGRMANKP
                     LDSNQELMAQGIGNLLLPFFGGIPATAAIARTSVAIKSGAQTRLAGMIHAVVLLLSML
                     VFAPVMSQIPMPALAGVLIVTAWRMNEWETIKELFTNKYWSAVLLFILTMICTVIFDL
                     SIAIVIGVIGGCVFFVVKSAAITISLETIDWQRMGLPETEKLNNWTVVYISGPLFFMS
                     SEKLKNKLEELNHHDGVIFSMRGVPSIDLTARAVFDEFQEKAEQRGQTIIYTSLQPEV
                     EKQLSHLWEHTFTHQHQTVAHALTALHAKYTAISH"
     gene            complement(49176..49817)
                     /locus_tag="U2K71_000048"
     CDS             complement(49176..49817)
                     /locus_tag="U2K71_000048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEM7314173.1"
                     /translation="MEYPKELIQEASLRMNGRPVEGFIAGQGPLHPKLMLVGEAPGKT
                     EIDTHVPFSGQAGKELMQALSSTGLTREEVYITSAVRSRPYRVTHRINKRTQQSETVY
                     PNRTPTRSEVFAHAPILDYELMKVQPKLIATLGNIGLQRLLGKEYTVTKNHGQLYTGP
                     VLQLTEQKDAYEWSEKNYRILPLFHPAAIFYNRSLVPVIHQDWQVLASLLQQY"
     misc_feature    50003..50079
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00559"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L21 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00559"
     gene            50099..50407
                     /gene="rplU"
                     /locus_tag="U2K71_000049"
     CDS             50099..50407
                     /gene="rplU"
                     /locus_tag="U2K71_000049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HEM7314174.1"
                     /translation="MYAIIKTGGKQVKVEVGQAIYVEKLDVEAGEKVVFDEVILVGGE
                     STKVGAPTVAGATVEGTVEKHGKQKKVVTFKYKPKKHSHRKQGHRQPYTKVVIDAINA
                     "
     gene            50434..50778
                     /locus_tag="U2K71_000050"
     CDS             50434..50778
                     /locus_tag="U2K71_000050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal-processing cysteine protease Prp"
                     /protein_id="HEM7314175.1"
                     /translation="MIKGTFKRNDAGQIVSFTLTGHADAGPYGSDIVCAGVSALAIST
                     VNGIASLAGFEPIVEMNEEEGGYLYTEVTSGMTQEQNNIAEILLENLLLGLQSIEAEN
                     SEYIQIKTINDK"
     gene            50791..51084
                     /gene="rpmA"
                     /locus_tag="U2K71_000051"
     CDS             50791..51084
                     /gene="rpmA"
                     /locus_tag="U2K71_000051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HEM7314176.1"
                     /translation="MLLSMNLQLFAHKKGGGSTSNGRDSESKRLGAKRADGQTVTGGS
                     ILYRQRGTKIYPGVNVGIGGDDTLFAKVDGVVRFERKGRNKKQVSVYPVAQEA"
     gene            51518..54325
                     /locus_tag="U2K71_000052"
     CDS             51518..54325
                     /locus_tag="U2K71_000052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM7314177.1"
                     /translation="MYVKEKTAHLTKAEYDQGVTTQEIAEVLGIQRTNSSKDLNQLVR
                     EGKLIKTDGRPVRYIYQTLVTHVKPVTKHVVSYKETPVSEEKNTLQIDTKDIFAKIIG
                     ANGSMKNSVEQAKAAILYPPRGLNCLITGPTGSGKTYFAHAMYHFAKAHHVIDEETEL
                     IVFNCADYANNPELLMSHLFGYAKGAFTGADEEKMGIIDQADGGMLFLDEIHRLPPEG
                     QEMIFYFMDHGTYSRLGETTKSHEANVRIVGATTEDPGSSLLETFVRRIPINIKLPAF
                     EKRPANEKVDLVKIMIAHEANRTQRKISLTEDVAKALIGSVTYGNIGQLKSNIQLVCA
                     RGFLNHMQSPEISITIDDLTEGIRSGLIQLASNRSAMAELSKILEPKITVAPNDTIMK
                     IQSDSYELPYNLYDIIGDKAALLKSDGLDQEAINHFISTDINIHLKSFYKDHGFSFNA
                     DSKLAEFVDPKIIEVTNQIYVMVKRSLPYEFQQNFIYAMSLHISSFLKKINLGEERHT
                     NDNIREMAFDFPDEYAVAKEVRSYIETYFQVEIPESEDYYLTVLLVSLRANQASGRIG
                     VVVAAHGNSTASSMVQVVQQLLDAENVRAVDMPLDMDPKTALDRIERNVQEVDEGSGV
                     ILLVDMGSLASFNSQIQRDTGIPVRTVDMVTTSLVLETVRKVSVLGTDLDMLHESLKN
                     FRGYAEISSVESVQEAKPIHKKKAVLAICASGEGTAQRIKELIERAVNKRQETELEVL
                     ALSIVEVKEELPVIQKEYQIIATTGITDLNIQAPFIPLERFIDQNIEVILDQLLMESE
                     LEETEFISLDEESAKNTCVEFISDNFIFINGSKLIDPMWQFSTQISQTTGIGDEEYGF
                     KINLVMHTAGMIERIIRNEPLTVEENELTNTTNDPLYSQLAASVVLLEDQIKVKVPIE
                     EMYYLLRLVHNQLDKKEYTVP"
     gene            54516..55007
                     /locus_tag="U2K71_000053"
     CDS             54516..55007
                     /locus_tag="U2K71_000053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7314178.1"
                     /translation="MDIRLARIDDRLIHGQVATVWSKQTGVERIIVISDVVAQDKLRK
                     FLLKEAAPPGITANVITVQKMIQIYHSGLLSRAKVMLLFTNPQDVEAVVRCGVQLKSL
                     NIGGMSFTEGKSMITNFVSVNQQDIDSFRYLNSQEVELEIRKVPADRKILLMELLAKI
                     KKV"
     gene            55110..56099
                     /gene="manX"
                     /locus_tag="U2K71_000054"
     CDS             55110..56099
                     /gene="manX"
                     /locus_tag="U2K71_000054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIAB"
                     /protein_id="HEM7314179.1"
                     /translation="MVGIILASHGQFAEGILQSGSMIFGEQENVKAVILKPSEGPDDL
                     RAKLEEAVASFDNQDEVLFLVDLWGGTPFNQSNTLFEEHKDKWAIVSGLNLPMLIEAY
                     ASRFSMESAHEIAAHIIETAKDGVKVKPEELEPAEAPKAAVEDAQPKGALPEGTVVGD
                     GKIKYVLARVDSRLLHGQVATAWTKAVQPNRIIVVSDAVSKDDLRKRLIEQAAPPGVK
                     ANVIPISKMIEVAKDPRFGNTKALLLFENPEDVLTAVEGGVDIKELNVGSMAHSIGKV
                     VVSKVLSMGQEDVEAFEKLEQKGVKFDVRKVPNDSRDNMDDILKKAKAELAKA"
     gene            56132..56935
                     /locus_tag="U2K71_000055"
     CDS             56132..56935
                     /locus_tag="U2K71_000055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter subunit
                     IIC"
                     /protein_id="HEM7314180.1"
                     /translation="MSIISIILVVLVAFLAGMEGILDEFQFHQPLVACTLIGLVTGNL
                     EAGIVLGGTLQMIALGWANIGAAVAPDAALASVASAIILVLGGQGVKGVPSAIAIAVP
                     LAVAGLFLTMIVRTIAVPIVHLMDAAAEEGNIRKVEMWHIIAVCLQGVRIAVPAAALL
                     FIPAQTVQSFLESMPAWLTDGMAIGGGMVVAVGYALVINMMATKEVWPFFVIGFVVAA
                     ISQLTLIALGALGVALALIYLNLSKMGGSSNNGGGSNSGDPLGDILNDY"
     gene            56956..57870
                     /locus_tag="U2K71_000056"
     CDS             56956..57870
                     /locus_tag="U2K71_000056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose/fructose/sorbose transporter family
                     subunit IID"
                     /protein_id="HEM7314181.1"
                     /translation="MAEEKIKLSKKDRLAVAWRSTFIQGSWNYERMQNGGWAFSMIPA
                     IKKLYKTKEERSAALKRHLEFFNTHPYIASPILGVTLALEEERANGAPVDDVAIQGVK
                     VGMMGPLAGVGDPVFWFTVRPMLGALGASLAMGGNILGPIIFFLGWNIIRWAFMWYTQ
                     EFGYKAGSKITDDLSGGLLQDVTKGASILGMFVLAALVERWVSIKFLPVVSTVKLDKG
                     AYIEWDKLPSGGEGIKSAFEQVNNGLALSPEKVTTLQDNLDQLIPGLAALGLTLLCMW
                     LLKKKVSPIVIILGLFVVGVVGHVIGLL"
     gene            57953..58327
                     /locus_tag="U2K71_000057"
     CDS             57953..58327
                     /locus_tag="U2K71_000057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF956 family protein"
                     /protein_id="HEM7314182.1"
                     /translation="MVQSINTKVDLVIDATSFTGLTDYGQIMIGDNGFEFYHARDRRK
                     YIQIPWEEVDYVLASVMLKGKWIPRYAIQTKKNGTYTFSSKEPKKVLRAIREYVDEDR
                     MVQSLSFFDVVKRGIRSMFKRK"
     gene            58466..58711
                     /locus_tag="U2K71_000058"
     CDS             58466..58711
                     /locus_tag="U2K71_000058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM7314183.1"
                     /translation="MSWLWTLIVGAVIGAIAGAITSKGNSMGCVTNIIAGLVGSSIGQ
                     AIFGEWGPKAAGMALIPSILGAVILVAVVSFFFGKRS"
     gene            58883..59947
                     /locus_tag="U2K71_000059"
     CDS             58883..59947
                     /locus_tag="U2K71_000059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEM7314184.1"
                     /translation="MMLRVEKLRKKMQEENLDSFLVTSPYNLRYLTNFTGTTGLAVIT
                     LEKAFFITDFRYTEQAAAQAQGFEIIKNVGPIFDEVANLVRKEELHSLAFEETTVSFL
                     EYSVLEEIIDAELVPVSQMIEELREVKDEEEIAIIEKACHIADMAYDHILKMIQPGMT
                     EIEVANQLDFYMRSLGASGVSFDTIVASGLRSAMPHGVASKKIIEQGDLITLDFGCYY
                     EGYVSDMTRTFAIGDPGEKLKEIYQIVLDAQLAVIDAAKPGMTGVQLDAVARDYISKH
                     GYGEAFGHSTGHGIGLEIHEGPNVSSRAEKQFVPGNVITDEPGIYLPGIGGVRIEDDL
                     LITEDGNRVLTRSPKELIIL"
     gene            60193..60618
                     /locus_tag="U2K71_000060"
     CDS             60193..60618
                     /locus_tag="U2K71_000060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM7314185.1"
                     /translation="MADEKNLILNANQDLGEIVIAPEVIEVIIGIAASKVEGVYGMRG
                     TFASNVTELLGRAAHGKGVYLANEEDGLKVDLYCYLEYGVSVPKVAMEMQERVSQQVL
                     FMTDIDLSEVNIHVVAVVPEKLEQPTIEELFEDEEEENE"
     gene            60611..61060
                     /gene="nusB"
                     /locus_tag="U2K71_000061"
     CDS             60611..61060
                     /gene="nusB"
                     /locus_tag="U2K71_000061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HEM7314186.1"
                     /translation="MNKELSRHEIREMALQALFPLDFNADLTKEDAIFNAIELDHRDM
                     INEDESEFVPVYLDTLVGGVCAKKDELDKVIEKHLKNNWHINRISKMDLVILRIAIFE
                     MTYVTDVPAAVALNEAIELAKTFSDDRSRKFVNGVLSNVLKELEAGA"
     gene            61157..62011
                     /locus_tag="U2K71_000062"
     CDS             61157..62011
                     /locus_tag="U2K71_000062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase"
                     /protein_id="HEM7314187.1"
                     /translation="MAELINGKELAEKMQAEIAEKIQKLKDNGIHPGLVVLLVGENPA
                     SQIYVRNKERVAKEIGIHSLVERYPETISEDELLAEIEKYNQDPSFHGILVQLPLPKH
                     IDEEKILLAIRPEKDVDGFHPMNLGRLLAGKPDKIPCTPYGIMKMFEAYSIDPAGKRA
                     VVIGRSNIVGKPMAQLLLMADATVTVAHSKTENLAELAQEADILVVAIGRGHFVTKEF
                     VKPGAVVIDVGMNRDANGKLIGDVKFDEVEPIAGWITPVPKGVGPMTITMLMYQTVKS
                     AEAFGAGE"
     gene            62012..63349
                     /locus_tag="U2K71_000063"
     CDS             62012..63349
                     /locus_tag="U2K71_000063"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HEM7314188.1"
                     /translation="MAQEYLTVTTLTKYLKRKFDADPYLERVYLTGEISNFRLRANAH
                     QYFSLKDDHAKISAIMFKGAFQKLRFQPKEGMKVLVVGRISLYEAGGSYQIYVEHMEP
                     DGIGALYQELEERKEKLMKEGLFQGPKKMLPRFPKRIAVLTSPSGAVIRDIITTVKRR
                     YPIVQLVLFPTVVQGNQAADNVVRNIQRVEEMGNFDTMIIGRGGGSIEDLWPFNEERV
                     ARAIYQANTPVISSVGHETDTTIADLVADVRAATPTAAAELAVPVLSEELMRIQERQA
                     RLQQAFVRQIQRKQERFERARRSYVFRQPERLYEGQTIKLDQLKQRLYQATQQIYHAK
                     EKQTIALSHQLEQVAPLYRVKAARQETAYLEKRLEEKIQQYMKQQQQRFAQAVQSLDL
                     LSPLKIMGRGYSYTTLNDQVVKSVTEIHQGDLLTIHYQDGIVKGQVQEIDKGE"
     gene            63354..63578
                     /locus_tag="U2K71_000064"
     CDS             63354..63578
                     /locus_tag="U2K71_000064"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HEM7314189.1"
                     /translation="MENPTFEESLQELEKIVMQLEQGDVPLESALDSFKRGMELSKHC
                     QDTLAKAEETLTKMMTESNEEVDFDGNEES"
     gene            63580..64470
                     /locus_tag="U2K71_000065"
     CDS             63580..64470
                     /locus_tag="U2K71_000065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEM7314190.1"
                     /translation="MRLTDFSAIHLPAVEKEILSFLDEHTTDRGLYDAMSYSVKAGGK
                     RIRPLLLLTAVASFDEPIDVPVYQVAAALEMVHTYSLIHDDLPAMDNDDLRRGKPTNH
                     KVFGEALAILAGDGLLTGAFQLISMAHLGNSPKLLLLQQLAVCAGSQGMVAGQAADIE
                     GESKKLSLEELAFIHERKTGHLIRYALLAGGILAKQPEEILLLLQRLAEHLGLAFQIR
                     DDLLDVIGTTKTLGKTAGKDERMEKNTYPRLLGLEKTREALEIELLSANKIIDKLEED
                     VFSFDGGLMRQMIKQFDVEK"
     gene            64479..65297
                     /locus_tag="U2K71_000066"
     CDS             64479..65297
                     /locus_tag="U2K71_000066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HEM7314191.1"
                     /translation="MEKERVDVLAVKQGLFETREQAKRSVMAGLIYNEKNERFDKPGE
                     KIPVSSELRVKGKKLPYVSRGGLKLEKALKAFDLSVEGKTLLDIGASTGGFTDAALQN
                     GAKMSYALDVGYNQLAWKIRQDPRVVVMERVNFRYAKPEDFTLGIPEVAVIDVSFISL
                     KLMLPPLHAILKEEGEVIALIKPQFEAGREAVGKNGIVRDPQTHQKVLEDILSFAAQG
                     GYDVLELSYSPITGGEGNIEFLAHLRKVPESGTINSAINMAEVVSNAHEQFDHK"
     gene            65404..65853
                     /locus_tag="U2K71_000067"
     CDS             65404..65853
                     /locus_tag="U2K71_000067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEM7314192.1"
                     /translation="MRKKDRHRLITRLLSEQDIRKQEEFVEILKSKGISVTQATISRD
                     IKELKLIKVPASDGGYRYSIPAETNEDVNTKLEKLLKDAFVAVDQMEKFVILKTLPGN
                     ASAAANLIDKRYKKELFSIINDDDNVLMITRTEDQAVLLKKDFLHYL"
     gene            65871..67553
                     /gene="recN"
                     /locus_tag="U2K71_000068"
     CDS             65871..67553
                     /gene="recN"
                     /locus_tag="U2K71_000068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HEM7314193.1"
                     /translation="MLQEISITNFAIIPELRLSFHEGMTALTGETGAGKSIIIDALGL
                     LAGGRGSSDYIRQGAEKCVLEGLFELPKQEGFSELMVELGIETDEDNLIVRRDMSLTG
                     KNVCRVNGHIITLANLRRIGSYLVDIQGQNEHQELLQPESHLALLDRFGDVAFQQKKK
                     SYQQEYISYRELEKRVRKVQQNEKSYVQRIDMLHFQQEEIAAADLQVGEEEKLKEERE
                     KLSNYQKIADGLAAGYGALTNNEQNSVDGVGLAVSELQGIAHLDVEYEAIYENIQSAY
                     YLLQDAIGDMSRQIDLLELDESRLEEVTQRLELIRQLKRKYGESIESILAYYDEITEE
                     LASSDFSEGQLDKMKEELEQKELLLQQQAADLHEARKKIAKELEKSILHELKSLYMEN
                     TEFEVRFLKEENRQLNHDGFDQIEFYITTNPGEPLKPLVKVASGGELSRMLLALKTIF
                     SSEQGVTSIIFDEVDTGVSGRVAQAIADKISQISKYSQVLCITHLPQVAAVADYQYYI
                     VKAVIDGRTQTSVSELKTKEREEEIARMLAGSEITKLTLEHAKELLKLAKRT"
     gene            complement(67608..67724)
                     /locus_tag="U2K71_000069"
     CDS             complement(67608..67724)
                     /locus_tag="U2K71_000069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4044 domain-containing protein"
                     /protein_id="HEM7314194.1"
                     /translation="MREKKQTSTFAKVTKFVIWTMLILTIGSVVLTAVMSVM"
     gene            complement(67797..68753)
                     /locus_tag="U2K71_000070"
     CDS             complement(67797..68753)
                     /locus_tag="U2K71_000070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEM7314195.1"
                     /translation="MIKYFTLDNEKLRNTGKETDQTIWYCVERPNQEEIHQLTHQFEI
                     PSDYITSILDDAENSRTEEFNQSKLTKPALLLLQYPFAKTSPSGYFQVDTYPLSIILT
                     PKKKLITITNHEPLFLKKVFSQTFPKSDISPNLNIFFQFIWQVTLSYNSYLATLQSQC
                     DTLEAKLQVSTENKQLYQIMDIQKSLVYFREATSANLDALKELAQNKLFQENHALKNH
                     LRDVLVETEQAMTTSRIQLKLVEQMNQTFSAIVSNNLNNIMKILTSLTIILTIPTIVG
                     SIYGMNIKLPIAERDDAFIWLVLLTLFICIVTTYYLKKGKFL"
     gene            68924..69292
                     /locus_tag="U2K71_000071"
     CDS             68924..69292
                     /locus_tag="U2K71_000071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3397 domain-containing protein"
                     /protein_id="HEM7314196.1"
                     /translation="MGTFTPLILFWYIYPVIVLFGCQFLVKLFSLNRRFKIKAPDLAI
                     PFLWGGMHALSRNTGTISILPFFFISVLLMGICIAVFQAYYYEEIIYPRYFKMTWRLV
                     FLLTIVLYAVLIIVNILSYL"
     gene            69462..69893
                     /gene="mraZ"
                     /locus_tag="U2K71_000072"
     CDS             69462..69893
                     /gene="mraZ"
                     /locus_tag="U2K71_000072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005881057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HEM7314197.1"
                     /translation="MLMGEFQHNIDAKGRLIVPSKLREELGEKFVLTRGLDGCLFGYP
                     MSEWENLEAKLNEMPLAKKDARTFVRFFYSAATECELDKQGRINIPSTLRNYAALTKG
                     CVVIGVSNRIEIWDETRWQDFSAAAEENFDEIAESMIDFGL"
     gene            69914..70873
                     /gene="rsmH"
                     /locus_tag="U2K71_000073"
     CDS             69914..70873
                     /gene="rsmH"
                     /locus_tag="U2K71_000073"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HEM7314198.1"
                     /translation="MTETFQHYTVMLKETVDGLNIKPDGVYVDCTLGGAGHSEYLLSQ
                     LSSKGHLYAFDQDQKAIDHAKLRLASYVEQGMVTFIKANFRELEERLSEHVDKVDGIL
                     YDLGVSSPQLDEAERGFSYHQDAPLDMRMDQSAPLSAYHVINEYSYHELVKIFFRYGE
                     EKFSKQIAREIERVRKTKPIQTTGELVEIIKQVIPAPARRKGGHPAKRIFQAVRIAVN
                     DELGAEEASLEQAIRLLKVNGRISVITFHSLEDRIVKSMFKEYSTVQDLPPGLPVIPD
                     EFQPELKVITRKPILPSEEELTENNRSRSAKLRIAEKIRMKEE"
     gene            70878..71279
                     /gene="ftsL"
                     /locus_tag="U2K71_000074"
     CDS             70878..71279
                     /gene="ftsL"
                     /locus_tag="U2K71_000074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsL"
                     /protein_id="HEM7314199.1"
                     /translation="MAELKKMQDYEYDLPELVDQPEQQMPDSSSNQEIILPQSPAKRL
                     KQISALEKIAVASVAIAIVALCVLTVMLRTNISGAEKEITSIQTEINDKKQEKTSLEQ
                     EKNELSRTERIKQIAEEKGLSINDDNLRKVK"
     gene            71280..73472
                     /locus_tag="U2K71_000075"
     CDS             71280..73472
                     /locus_tag="U2K71_000075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEM7314200.1"
                     /translation="MSLKNKIRRFMEKKNLNPMNNRKKVGIILFATSIGLFFLFAVRF
                     SYIVIGGHVAGTSLAEKTKQLYQGSEVVKAKRGTIYDRNGVALAEDASSYSIKAILSK
                     TYTSGDKKLYVEEKNFDKIAEILHKNLSIDKKDALNILEDGAKKELYQVEFGSYGKNI
                     SQETKQNIEADMKKEGVAGLYFVDHQARMYPNGVFSSHFIGYAVPDKDENGLVGKLGL
                     ESAYNDILSGKDGKIIYQKDNFQNPLPGTVAEEEKAVDGQDIYTTLDSRLQSYLETLM
                     DQVNEEYQPEELTAVLMKAKTGEILAMGQRPTFNPETMEGLTGKDAIWRNFLVQDSYE
                     PGSTMKVFTTAAAIEEGEFNENETFQSGKIQVEDATINDHDFGEKGVLTMRQALSWSS
                     NVGMVILEQRLGGRWYNYLQKLGFGQSTHSGLDDEVNGALPTLNIVDRAMSAYGQAVG
                     VTNFQMMKAFTSIANNGTMIQPRYISKVVDPQTGEERTTQTEVLGQPFSKETTEKVRE
                     YMRDVVESENYGSAYGVYSVPGYNVSAKNGTAQIASDTGGYQTGDTAYLYSIVEMVPS
                     EDPDYVLYLTMKHPKTYDRMALAKIANPLMKRAMDFKETEEDSDTETKTEKVSVADYR
                     NLEADVAAADAQKSGLQPVVIGNGKKVQKQSTANGDQLISGEKLILYTGGDKLMPDVT
                     GWSKADIMKLGKILGIEVSFDGDGYCVKQELAPYEKITEDKLSFTLEE"
     gene            73498..74460
                     /locus_tag="U2K71_000076"
     CDS             73498..74460
                     /locus_tag="U2K71_000076"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HEM7314201.1"
                     /translation="MEWTQALIPIVSSCAMTIAAMPLFIGYFQMKKQGQAIREEGPKW
                     HNSKAGTPTMGGLVFLIGSILTGIWVGAWQKQLTPTLFILLFVLALYGVIGFLDDFIK
                     IFKKRNMGLNSKQKLIGQILGGIIFYLVYRSEGYPGVLNFFGLDLPLGIIYGIFALFW
                     LVGFSNAVNLTDGIDGLVAGLGSISFAAYGLIAWHQHQYDVLIICLSVLGGLLGFFVY
                     NRKPAKIFMGDVGSLALGGLLAAISIMLNQEWTLLLIGLIYVMETASVMLQVTSFKLT
                     GKRIFKMSPIHHHFEMCGWSEWKIDTVFWLISIVTSLITLWIVW"
     gene            74474..75835
                     /locus_tag="U2K71_000077"
     CDS             74474..75835
                     /locus_tag="U2K71_000077"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HEM7314202.1"
                     /translation="MKKISTYENKKVLVLGLAKSGVSAAKLLHELGALVTVNDGKPFD
                     ENPEAQELLSLGIKVITGSHPIELLDEEFSLMVKNPGIPYSHPLVAKAQEMGIPVITE
                     VELAYEVAECPIIGITGTNGKTTTTTMTGLLLNAGADQGIARLAGNIGYPASGVAQEA
                     KSEDKIVMELSSFQLMGITDFRPHIAVITNIYEAHIDYHGTRKEYVKAKWNLQKNMTE
                     KDYLILNWNQSELQELAQRTKARVLPFSTKEVLEDGVYADDYSIYYKKEKIMEISELG
                     VPGKHNVENALAAISVAKLYGISNEAIRETLHFFHGVPHRTQYVGEIQGRKFYNDSKA
                     TNILATKMALSGFETSKVVLLAGGLDRGNTFDELIPSLKGIKAMVVFGQTKEKLMDAG
                     KKAGIETIVTADSVEQAVPLALENSTDGDVVLLSPANASWDQYPNFETRGNRFMEAVN
                     RLK"
     gene            75861..76949
                     /gene="murG"
                     /locus_tag="U2K71_000078"
     CDS             75861..76949
                     /gene="murG"
                     /locus_tag="U2K71_000078"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase"
                     /protein_id="HEM7314203.1"
                     /translation="MKILVTGGGTGGHIYPALAFVNYVKTQEPNAEFMYVGAKRGLEN
                     KIVPDTGMPFHTLEIQGFKRKISMHNIKTVRLFLKSIRQAKKILSDFQPDIVIGTGGY
                     VSGAVVYAAAKMGIPTVIHEQNSVPGVTNKFLSRYVDRIALSFKDAASFFSENKAVLV
                     GNPRAQEVADTKKSDILKTFDLDPEKKTVLIFGGSQGALKINQAVSVFLSEYIPSSYQ
                     VLYASGDRYYEEILNKINPEIQNISVQPYIKNMAEVMASSDLLVGRAGATSIAEFTAL
                     GLPAVLVPSPYVTNDHQTKNAMSLVHAGAAKMIADNELTGESLSQTVNEIMGDEELQK
                     QMCRASKEQGIPDASKRLYDLVKQIIKK"
     gene            76964..78184
                     /locus_tag="U2K71_000079"
     CDS             76964..78184
                     /locus_tag="U2K71_000079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsQ/DivIB"
                     /protein_id="HEM7314204.1"
                     /translation="MSKKKEPKKLSHPSSDENNLTPWQKANRKYLAEQEMKNQEPKKE
                     DITLDTPLSDSEEEKTVQEEQQEESQSEKKIEFFEEIKQKKNGGPYNGSFLNRLPNLK
                     SQRHKVLFRRLALIITVLGIPLIFLIYYVSPYSKLQAVAVSGNKTVNSQEIISDTKLS
                     LGENVWGQYFHRSTYIDRLKKAQPRIETANIHFKGMNEFDLDVTEYKEIALIAKNNQY
                     YPVIENGTVLDEKVANPTKNLPILEEFKDSAKIKELTKQYNQLSSELQKAISEIKYTP
                     RASNKNLIQLNMNDGNQVIVNINNLANQMKYYSQVAKDMDEKGVIDMEVGIFSHPYGS
                     TQETEESGQSEETTESEENADQTNESSEEAETQKAQTPGSSSTNGSSDSNDSSETSQI
                     EDNPSTTPSSLNEN"
     gene            78340..79665
                     /gene="ftsA"
                     /locus_tag="U2K71_000080"
     CDS             78340..79665
                     /gene="ftsA"
                     /locus_tag="U2K71_000080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HEM7314205.1"
                     /translation="MAKTGMYVGLDIGTTSVKVVVAEYIDSQMNIIGVGNAKSEGINR
                     GIIVDIDKTVQAIQRAVRQAEEKAGIQIKGVSVGLPANMLEVENCQGMIAVNGDSKEI
                     TDEDVRNVASAALVRSIPPERQIVSILPQDFTVDGFEGIKDPRGMIGVRLEMYGLLFT
                     GPKTIIHNIRKCVENAGLIVNEMVITPLALTESILSDGEKDFGTIVIDMGGGQTTTAV
                     MHDKQLKFTNLDQEGGEFVTKDISIVLNTSFNNAEALKINYGDAYPERTSPDEEFPVD
                     VIGQSEPVKVDERYLSEIISARMEQIFNKAKDALDQIEALELPGGVVLTGGAASLPGV
                     VDLAQEIFGVNVKLYVPNHMGLRNPVFTNVISIVDYSANLSEVYQLAKIAVTGETSAA
                     RQMVVEQETTNTYESYEAPEETIYDEPEPKESGENVKNKIKGFFTNIFD"
     gene            79688..80929
                     /gene="ftsZ"
                     /locus_tag="U2K71_000081"
     CDS             79688..80929
                     /gene="ftsZ"
                     /locus_tag="U2K71_000081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HEM7314206.1"
                     /translation="MEFSIDNNINDGAVIKVIGVGGGGGNAVNRMIEENVKGVEFITA
                     NTDVQALKNSKAETVIQLGPKYTRGLGAGSQPEVGQKAAEESEQSLREALDGADMIFI
                     TAGMGGGTGTGAAPIVAGIAKELGALTVGVVTRPFTFEGPKRGRFAAEGIARLKENVD
                     TLLIISNNRLLEVVDKKTPMLEAFREADNVLRQGVQGISDLITAPGYVNLDFADVKTV
                     MENQGTALMGIGVASGEDRVVEATKKAISSPLLETSIDGAEQVLLNITGGLDMTLFEA
                     QDASDIVANAATGDVNIILGTSINEEMGDEIRVTVIATGIDESKKERKSSRPARQAQM
                     QSPAQKTVLDMDQAKPTSSEEENSFGDWDIRREQNVRPRVDDSNFDNIEKKEFDTFNR
                     EETKSHGDDELNTPPFFRRKK"
     gene            80946..81623
                     /locus_tag="U2K71_000082"
     CDS             80946..81623
                     /locus_tag="U2K71_000082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HEM7314207.1"
                     /translation="MIADNLQKIKQEIQDSCALVHRDPKEVTLVAVTKSVESAQAEEL
                     VRAGVMNCAENRVDKLLEKKQDLFDFPEIKWHLIGNLQRRKVKLIVNEIDYFHALDSL
                     RLAEEIQKRLEKELSCFIEVNVSGEESKHGISPDELLPFVESLAACTKIKVVGLMTMA
                     PKEASDEQIHKVFGTLKELRDLVQAKGYAHAPCSELSMGMSQDFQIAIEEGATFIRVG
                     TALFKDE"
     gene            81637..82236
                     /locus_tag="U2K71_000083"
     CDS             81637..82236
                     /locus_tag="U2K71_000083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein SepF"
                     /protein_id="HEM7314208.1"
                     /translation="MPLLNRGAIANFFGLADEEEYEYQDYPETTKKAVQPTKKNTTQN
                     QSTHSVSAANTHEKQKTATSRPVTHESIQPAEKKKVIELHQSPTKTVAKERTASRTST
                     QPKANLAGKITVMEPRAYSEAMAIAKRIIAGEATLVNFHLVEEKQARRIVDFLTGTVY
                     ALDGDIKRVGDEIFLCTPTGMEIDSSTAQSFADANLFDL"
     gene            82251..82538
                     /locus_tag="U2K71_000084"
     CDS             82251..82538
                     /locus_tag="U2K71_000084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HEM7314209.1"
                     /translation="MYTIALLLHLLQRAAYFYSIILMVYALLSWFPGGYQSGLGRLLA
                     KICEPYISLFDRLPLRIGPLDFTILGAILILNLAVQGLAQIVIWITVHLVY"
     gene            82571..83368
                     /locus_tag="U2K71_000085"
     CDS             82571..83368
                     /locus_tag="U2K71_000085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HEM7314210.1"
                     /translation="MGGENFDANVYQHFRADERTFIDSVGDWIEQVQGQYAPYLTEFL
                     DPRQSYILETLIRQSSDLRFMFYGGYEQAERKRCLIFPDYYEPVEEDFETALYEIHYP
                     SKFASLSHGKILGTLVGTGIKRSYFGDIISDGDRWQVFIANEVDHFVAAQVTKIGKVA
                     VRLEPIKYTEMIMPKDSWTQERGTVSSMRLDSVISEIYNISRQRSKQLIEAGKVKVNW
                     TENTRPDFVLELLDIISVRGYGRVQIQELEGKTKKEKYRVLFGVLRK"
     gene            83389..84096
                     /locus_tag="U2K71_000086"
     CDS             83389..84096
                     /locus_tag="U2K71_000086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DivIVA domain-containing protein"
                     /protein_id="HEM7314211.1"
                     /translation="MALTPLDIQNKTFPTKMRGYNQDEVDDFLDLVVRDYEELTQRNR
                     ELEKAVKHSEEKLEYFNELKDALNQSIIVAQDTADKVKTSASKESEVIVTSAQNKADE
                     LVANAEKRAHQLTTDAEEKARKILTDATEKARQLATETEDLKKKTRVFHQRISLMLES
                     QLEQVKSPEWDEILQPFSSYVTDSHEVIKEVLAKQLDNENDTDVNSDTGVIEAPMTAF
                     DTQAIDISIVPGENENE"
     gene            84358..87156
                     /gene="ileS"
                     /locus_tag="U2K71_000087"
     CDS             84358..87156
                     /gene="ileS"
                     /locus_tag="U2K71_000087"
                     /EC_number="6.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoleucine--tRNA ligase"
                     /protein_id="HEM7314212.1"
                     /translation="MKMKETLQLGKTKFPMRGNLPNREGEWQKEWEENRIYQKRQELN
                     EGKPSFVLHDGPPYANGNIHLGHSLNKISKDIIIRSKSMSGYRAPYVPGWDTHGLPIE
                     QVLANKGVKRKEMCMAEYLEKCREYALSQVDKQRADFKRLGVAGDWEHPYVTLDPSYE
                     AAQVRVFGKMAEKGYIYKGLKPIYWSPSSESSLAEAEIEYKDVKSPSIFVAFNVKDGK
                     DILDEDTAFVIWTTTPWTLPANQGIAVNPKFTYVLVEADGRKFVVAKDLLETVQSEIG
                     WENVTVLKEFAGQEMEYMTASHPFYERESLVILGDHVTLDAGTGLVHTAPGHGEDDYI
                     AGSKYKLSVVSPVDSKGLFTEEAPGFEGIFYDKANPMITDLLKEKGALLKLDFFTHSY
                     PHDWRTKKPVIYRATPQWFASIDKFRQNILDEVEKVEWVIPWGKTRLYNMIRDRGDWV
                     ISRQRAWGVPLPIFYAENGEAIITPETIEHVAKLFAEHGSIIWFEKEAKELLPEGFTH
                     PGSPNGEFTKEKDIMDVWFDSGSSHEAVLRQRPELSFPADMYLEGSDQYRGWFNSSIT
                     TSVAINGVAPYKSVISQGFALDGEGRKMSKSLGNIIAPEKVIKQFGADILRLWVSSVD
                     YEADVRVSMDILSQVAEVYRKIRNTMRFLLANTEDFDPKKDTVSYNDLRSVDKYMTVR
                     LNQVIKEIREEGYDKYNFMHIYRTVMNFLTVDLSSFYLDFAKDVVYIEAEDDYQRRCM
                     QTVFYQTAVALTKLLTPIIPHTAEEIWSFLKEEEEYVQLSEFPGYEEFANQEELMDTW
                     TAFMDFRDNVLKALEEARNSKLIGKSLEAKLTVYPKQQVREMLKALDADIAQLLIVSP
                     DYFEVMAADEQVPDNAMVFDDVAITVEKADGETCDRCRQVRKDVGVDEKLPHLCGRCA
                     KIVEDFYPEAVAEGFEEK"
     gene            complement(87205..88731)
                     /locus_tag="U2K71_000088"
     CDS             complement(87205..88731)
                     /locus_tag="U2K71_000088"
                     /EC_number="1.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HEM7314213.1"
                     /translation="MSNEKNVLFTIFGGTGDLAQRKLYPSLFRLYRKGNLGEHFAVIG
                     TARRPWSDEHYREVVKETIRALNPSDEEATTFASHFYYQSHDVNDSTHYQTLKELSDR
                     LDGHYQLNGNRVYYLAMAPQFFGTIVSHLKSQNIMTKNGFNRLIIEKPFGSDYQSAFE
                     LNQQIREVFPEQDIFRIDHYLGKEMIQNISAIRFANNIFEAQWNNRYIDNIQITFGEN
                     LGVEDRGGYYDHSGALKDMVQNHILQVVALLAMEPPVAFSEKEIRTEKIKALKAIRIY
                     NEEEVLENFVRGQYAQGELDGVQFKGYREEDKVDAQSETETFVAGKFTIDNFRWSGVP
                     FYVRTGKRLTEKGTRINIVFKQVPVNVFKTSVDEPCDDTTLPPNVLTIYIQPTEGFSL
                     SLNGKEVGQGFETEPIKLEFRNSAEMVENSPEAYEKLLLDALNGDGTNFSHWEEVAQS
                     WHIVDVIRKAWDQTQPDFPNYKAGSMGPKAAFELLEKDGFEWIWQPDNWYRERGKLND
                     "
     gene            complement(88746..89393)
                     /locus_tag="U2K71_000089"
     CDS             complement(88746..89393)
                     /locus_tag="U2K71_000089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent transcriptional regulator"
                     /protein_id="HEM7314214.1"
                     /translation="MTPNREDYLKIILELGGDTTKVNNKQIVSSLDVSAASVSEMISK
                     LVKEELVEHSPYQGVQLTASGLQKASSLIRKHRLWEVFLVEHLDYSWNEVHDDAEVLE
                     HVTSEHLADHLENYLNHPEHCPHGGSIPKKEEVVHEERRQTLESYPVGTKVRIARVLD
                     EKELLDYLVTIDLNINEEYEIVDIAAYEGPITIQNDRKKIPVSFKAASTIFVDKI"
     gene            complement(89577..89906)
                     /locus_tag="U2K71_000090"
     CDS             complement(89577..89906)
                     /locus_tag="U2K71_000090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4828 domain-containing protein"
                     /protein_id="HEM7314215.1"
                     /translation="MIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQ
                     KQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGFYLIFSYPEQTLYDESADTS
                     YKLLSEK"
     gene            90083..90811
                     /gene="sfsA"
                     /locus_tag="U2K71_000091"
     CDS             90083..90811
                     /gene="sfsA"
                     /locus_tag="U2K71_000091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA nuclease SfsA"
                     /protein_id="HEM7314216.1"
                     /translation="MKYQQVEIAFFIDRPNRFIAHCMKKDGEIIIAHVKNTGRGKEVL
                     IPGAEVAIVFAPGPKRKTAYDLIAVKKKGDWFNIDSQLPNRLAIDGILDGTILLPGLT
                     GPFTVYKREVTYLHSKFDIYLETVVGQKAFVEVKGMTLENKAIGAFPDAPTLRGLKHI
                     GELTYAAQDGYAAYVLFIAQFEHLHLATIHEEMQPALADMVRHAQQSGVQILAYNCQV
                     SADEVTVKQAIPFDVDIPFEDPIQ"
     gene            90827..91840
                     /locus_tag="U2K71_000092"
     CDS             90827..91840
                     /locus_tag="U2K71_000092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM7314217.1"
                     /translation="MINLGIIGTNWITHQFVQAALSTKKYELTAVYSRHLEKAQEFGS
                     HYEGDIEFATDLKTFFNITHMDTIYIASPNSLHFSQAKQAILAGKNVIVEKPAFSTPK
                     EMDEIIHLANEHNVYFFEAARNIHESGFKKIADYLPLKDEIIGANFSYMKYSSRYDQV
                     LDGEEPNIFSTHFSGGALMDLGVYLVYAAVAWFGMPKEVHYFPRKISTGVDGLGWGIL
                     RYENFDVSIQPGKIGDSYLPSEIYFDSGTLVMNGVNAIEQAQFHDRAHKEIIDLAVTA
                     AENPMADEAEDFANVMAHPKDPAWGLLYEEWIELARNVNQVIYDLRKNGGIKFDADNE
                     EDF"
     gene            91840..93117
                     /locus_tag="U2K71_000093"
     CDS             91840..93117
                     /locus_tag="U2K71_000093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM7314218.1"
                     /translation="MNEFVQVFPENWQKKYEDKGFISPSLIQRAVFQPLSNKQSIVAV
                     SPTGSGKTLAYLWPLLLNVEPGEASALVIFASSQELAIQVADVAREWGKDKELKVQSL
                     VGGANVKRQIEGLKEKPEILIGTPGRILELMKAKKIKAHQVKTMVFDEADQLFDAGNS
                     QIIDQILHQAPTEYQLAFFSATADRSLESIEKITGKTFETIDVTAEDDSRKGLRHYFL
                     RVPIRKKDDYLRRLTHIEDFHGLIFFNQLTELGVMEEKLLYRGVPVGSLASDQNKLLR
                     KAALEQFKKKMVRALLTTDVAARGLDITGLPYVVNAEVPLSEEAYLHRSGRTGRMGNE
                     GIVITLVQDNNLRDLKRLLRPSNITLEEIFLYGGKLQTEQPTKEDAAPAVNKKYAKTF
                     SGKEQNPEYNKEKIRKKVKNKSKKERNKGARRK"
     gene            93180..95882
                     /locus_tag="U2K71_000094"
     CDS             93180..95882
                     /locus_tag="U2K71_000094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM7314219.1"
                     /translation="MKERQEEILQLLTENQQGLTASDVAERLTIDRSNASRYLSELYK
                     AHHIVKTAGRPVVYSLPTEKSKSDEVHVDSSTQVTFETLVGENDSLKVSIQQAKAAIL
                     YPPRGLHTIIFGETGTGKSMFAECMYHFAIDSEMLSADAPFVSFNCADYAQNPQLLFG
                     HIFGIKKGAYTGAAQDSPGLIAKADGGILFLDEIHRLPPEGQEMLFTFIDKGIYRPLG
                     ESAQVHEASVQIIGATTESSESFLSTFNRRIPMAITLPNLASRSLDERYEIISLFIKQ
                     EANRLNQRIDIEKEAILAFMLYDAEGNIGQVKRDLKLVCAKSFLHYRTHHEKKLIIRK
                     EELSLQVQKGLLKIKEIPERLDRFMDSKSQYLTFEPGFADVVWSQDPERNMQVYNDIE
                     EKVTSLSETGVENIDLETLISKDVDAYFQTYVKELTKSSIPKDLLPDEIWRLTNQLYD
                     VAEKRLDRIYNEKARFAFALHLQSTLDRVREGHMIVHPDLNNVRKNFKREFQVAIDLS
                     TIIEEEEQVEIPFDEIGFISMFLSINLGESEPVKENKVDVLILMHGRQTASSMLEMAQ
                     ELLGTKVGKAFNMPLVMEVQTMYEEVLQYVKNYQEHLTNGLLLLTDMGSLNTFSDLIY
                     EETGIRTKAITMTSTMIVIEAIRMASVGRSLEDIYQNIQLSFESIVRDQFKAEKSETL
                     QRKKAVIVTCFTGEGVAAKLYQRIMPVINQNKVELIQMQFLERESFKKHIDALLEEYE
                     IKAIAGTVDVDYQNIPFFSAYDVFDDEKLNILKRIADDEIPTAKIVDSLAGSLSDVFS
                     IDELIRSLQKTVQQIQTDLSIIVEPGVDTGIVIHLAFMLDGLIKQESHRQFEKLSIFS
                     KIYRLEMDIVRANLMALERKYQVKIPEDDIAFLTQMFLENKIKK"
     gene            96034..96351
                     /locus_tag="U2K71_000095"
     CDS             96034..96351
                     /locus_tag="U2K71_000095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7314220.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEEKGMEADIFAVSASDA
                     DNNLESKNVDVLLLGPQVRFMKAQFEQKLAPKGIPLDVINMQDYGMMNGEKVLAQAEN
                     LMK"
     gene            96381..96701
                     /locus_tag="U2K71_000096"
     CDS             96381..96701
                     /locus_tag="U2K71_000096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM7314221.1"
                     /translation="MEDQQNLEAIMGLIMFGGNAKSDAMEAIAAAKIGDFELADRKIA
                     DAEESLIQAHHAQTDMLTQEAQGNHMQVTLLTVHSQDHLMTSIAFTDLAKEIIELYRR
                     VYSE"
     gene            96809..98269
                     /locus_tag="U2K71_000097"
     CDS             96809..98269
                     /locus_tag="U2K71_000097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7314222.1"
                     /translation="MNGLSSWMEKYILPVAAKIGAQKHLVALRDAFIGILPATMAGSV
                     AVMINAIIRDLPQQFNSTYADSLAADKGIFAVLNVIIGINGFVWNGTLAIAGLIFAFS
                     WGYNLARAYGVNDLAGGIVGLATLIQGISFGPALSAALDVNVPSNVADAINSSEIGAT
                     IADGTLSVGLWGWLKLDHLNGNAYFTVMIMGAISVIIFCKLMLANITIKMPDSVPPAV
                     SKAFAAILPATIALYVIAIINYIVGRVADGQLIIDLVQKYIAEPFLGLSQGLGAVLIV
                     TVFVQVFWFFGIHGSNVLAPVLEGIWGQAQLINIDIFQKGYNGKTGTPAVLDAIEDGK
                     AYMWVRGSFDAFAWFGGSGGTIVLLIAILIFSKRADYLTVAKLSIGPGIFNINEPVMF
                     GLPIVLNAIMFIPFLIAPVVATAIGWTATYLGLVAPVSQQVTWVVPPFLLSFLATGAD
                     WRAPIVTLVCMVVTFLIWAPFVISANKMDPAAGEQQ"
     gene            98337..98558
                     /locus_tag="U2K71_000098"
     CDS             98337..98558
                     /locus_tag="U2K71_000098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314223.1"
                     /translation="MITIASAMQQDEIKELLEGYNEEGMTFTFKEKQGIKLFFETSAE
                     DPEVAAKKAKELIKAQPWGTVLYFQSTAV"
     gene            98589..98771
                     /locus_tag="U2K71_000099"
     CDS             98589..98771
                     /locus_tag="U2K71_000099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3188 domain-containing protein"
                     /protein_id="HEM7314224.1"
                     /translation="MIKNGLFLCSIGSLIVLYSLNPHSQRYDLYSMVTGLFLTGLGIY
                     FFMKGKKREEAKEKNN"
     gene            98771..99184
                     /locus_tag="U2K71_000100"
     CDS             98771..99184
                     /locus_tag="U2K71_000100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3284 domain-containing protein"
                     /protein_id="HEM7314225.1"
                     /translation="MEITKKLNIPASFFYDKVMDSVLFDVRKATGKSVTRKQLKNLEY
                     VKQFSQNNRARIKIEEVIENQSYKFRTSTTKNEFVVHYQIKPLDDSTCEVHYTEKMES
                     YGMLQKLNDMLLGTILMYFKRRRFKKMLDMIEATY"
     gene            99307..100488
                     /locus_tag="U2K71_000101"
     CDS             99307..100488
                     /locus_tag="U2K71_000101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7314226.1"
                     /translation="MTNKVRELGVSSIEEMFDLATYAFNGADTPERRERFRTLAENSW
                     NYGFFDEEDRLTSQVMATPFPVNFYGQEYLMAGIGYVASYPEARGQGGINQIMEKILE
                     DCRDRKVSLSYLAPFSYPFYRRYGYEQSFDKISYQLNSRDVPFIKKKSGKIKRMDFED
                     AKAAIRRIYEQMPENQRAGLKREDWWYTYKFKQKGNYQFALHFDDNEEATGYIVYQLA
                     TSSFIIAEWGFLDHDAFCSLVRFVSSHNGAFETFEYSCGYGGNDQNYLLENPFASVMI
                     TPYMMARIVDISLFLENYPFKKRQAAFALQIEEDHYAKWNEGIVEVTIENGKNHLSKV
                     EQTKLPIVRGSIQHITQLLLGYRKVEELVFQERIQVTKEYEETLSSILPQQQPILNDY
                     F"
     gene            complement(100559..100723)
                     /locus_tag="U2K71_000102"
     CDS             complement(100559..100723)
                     /locus_tag="U2K71_000102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314227.1"
                     /translation="MNDKPKEKVYAERLDSSKKNREKITKEAIQDHKKENPKTKENKK
                     FKFTKKEDLL"
     gene            100877..100950
                     /locus_tag="U2K71_000103"
     tRNA            100877..100950
                     /locus_tag="U2K71_000103"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:100911..100913,aa:Arg,seq:cct)
     gene            complement(101019..102158)
                     /locus_tag="U2K71_000104"
     CDS             complement(101019..102158)
                     /locus_tag="U2K71_000104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM7314228.1"
                     /translation="MARLVKRGNSWQYEISYKKDDGKYTKIRKSGFKTKGEAKDAANE
                     LEYNLNKGLKGDRKNLLLSDYFEDWMQLYKEGTVSPITYRKYEDTLMNIKKYMPAVLI
                     SDLDRVGYQRFLNKYAKDHVKSTVIKFNNHIRASLKDAVEEGLIPFDPTRKAVIKGKD
                     SLKPKEDKYLDYDQFKSLMKLVEENLSAQYSSPMLVLVAGATGMRFAELLGLTWEDID
                     FEDQIITINKTWNYKLNEWGKTKNETSNRKISIDKHTIDLLKKFKINQKELFENFEIK
                     NPHNFVFFNLKNGLVSSNAVSKYLRKKLKELGIEKQFTLHGLRHTHASILLYQGVNIL
                     SVSKRLGHSSLETTMSTYLHIVRELEDQDKEKINTVFDSLYKNDN"
     gene            complement(102272..102856)
                     /locus_tag="U2K71_000105"
     CDS             complement(102272..102856)
                     /locus_tag="U2K71_000105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4352 domain-containing protein"
                     /protein_id="HEM7314229.1"
                     /translation="MAKKVMGQDGKMYKVSKPFYKKVWFWVLAVILIIIIGSALNGGS
                     DSNKASDNGGEKVTKSSTSASSSKEEKSDTFYKIGDTVKVGDAEYTLNSVELTDERNQ
                     FEENQPAQVVKITYTVKNDGDSDIPVGTDVEVYGPDDKKSETYANENTMGSVAPGKQM
                     DVTAHFTLNQKGEIEIHFSPLVSFEKAAIFKATV"
     gene            complement(102939..103361)
                     /locus_tag="U2K71_000106"
     CDS             complement(102939..103361)
                     /locus_tag="U2K71_000106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM7314230.1"
                     /translation="MYLKLKEMLSEYNLKLIYMEMEEPGFYYPKPRIVFLNEKLHEDS
                     SEAFHLAHELGHFIASHFEYSVLYDNSTTFHSKFETEADKIAIMILLNIFIENELTDE
                     SQFKLENFMEFYAINNKLRTECFNVCQSYFKKKYSYAQ"
     gene            complement(103379..103699)
                     /locus_tag="U2K71_000107"
     CDS             complement(103379..103699)
                     /locus_tag="U2K71_000107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7314231.1"
                     /translation="MNTYEIIKDLAKRKKISIRQLEINFGYSNGYLAKWKNNTPNSDE
                     LSRIADYFDVSVDYLLGREERETPKHVDLSEDDTVFSFDGKEISKETMRKAIAIAKAL
                     EENE"
     gene            103998..104774
                     /locus_tag="U2K71_000108"
     CDS             103998..104774
                     /locus_tag="U2K71_000108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage antirepressor"
                     /protein_id="HEM7314232.1"
                     /translation="MNTPQIFNFEQNEVRTILVNDEPYFVGKDVASVLGYSNTKDALS
                     RHVDLEDKMGSRITTSGQSREMTIINESGLYSLILKSKLPSAKKFKRWVTSEVLPAIR
                     KHGGYLTPEKVEEALLNPDTIIQLATQLKEERTGRLIAEQKIAEYEPKISYLDSILSS
                     TDSVTISQIAADYGMSPQQMNKLLHKLGIQKKVGNQWLLCKKHMNQGYTKSHTTEIPK
                     ADGGTKIVMNTKWTQKGRLFIYELLKKEGYYPQMDLEEIG"
     gene            104789..104992
                     /locus_tag="U2K71_000109"
     CDS             104789..104992
                     /locus_tag="U2K71_000109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7314233.1"
                     /translation="MTDIAEITQRDREKIKEYVESSKFLTYTMLAERFGISKSYLSLI
                     LNGKKTSAEANRIIDSIITMYEL"
     gene            105008..105346
                     /locus_tag="U2K71_000110"
     CDS             105008..105346
                     /locus_tag="U2K71_000110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF771 domain-containing protein"
                     /protein_id="HEM7314234.1"
                     /translation="MQYLEAKIPIPEGYVIISQVDYEELKKADDTGRWMTLPEVLERI
                     NRKYDWFTSRVLKNPRYRNIIDIEKNKNGFVYYPVEGRDTYLFLRSKTLEFLETNFSE
                     ILRRQADGKI"
BASE COUNT        34449 a        18019 c        22124 g        30802 t
ORIGIN      
        1 aataaaaaca ctaataaagg atagtacagt ataataaaaa ataaaaggta tctggtacga
       61 aaatcaacta gatacctttt ttgtgttctt atttttttgt tattttataa aaaatagaga
      121 tctcatgtta gaaaacctaa catatgcagt tgacaatatt ttttttaatt ggtacacttt
      181 agccaactta taaaacgaaa ggggacgtct gatgggagaa aaggaattgc gccgatctat
      241 gtccgtattt ccaattggta cagttatgaa gctgactgat ttgtctgctc ggcaaattcg
      301 ctattatgaa gaacaagatt tagttcatcc ggaacgaagc gaaggaaacc gacgtatgta
      361 ttcgctgaat gatattgata tgttgcttga gattaaagac tatctttcag agggattgaa
      421 tatggctggg atcaaacgtg tatacgaaat ggcacaggcg aaaaaacaag aagcaaaaaa
      481 caagaagccg ttgacagatc atgacgttcg acaaattttt cgtgatgaga ttttggcgca
      541 aggtgggtta agcaaaacag gtcattatca gtcgcaagga ttgcaaagac aattctttga
      601 ctgaataaat taaaaaagga agagggatac catggtaaag aaacagataa caggtgaaga
      661 aatcaaacga atcattgagg aagaaaacgt acgattttta cgattgatgt tcacagacat
      721 cttaggtacg atcaaaaatg tagaagttcc agtgagccag atcgacaaag tattagaaaa
      781 taaaatgatg tttgacggtt cttcgattga aggatttgtt cgaattgaag aaagcgatat
      841 gtatttgtat ccagatctct ccacttggat gatcttcccg tgggaaagtg ctcatggaaa
      901 agttgctcgt ttaatttgtg atatctataa tccggatggg acaccatttg ccggcgatcc
      961 tcgcgggaat ttgaaacgag cattgaaaga tatgcgcgac cttggtttta cctctttcaa
     1021 tttaggacct gaaccagaat tcttcttatt taaattagac gaagacggtg ggatcacaac
     1081 gaccttgaat gataaaggtg gttattttga ttttgcacca acagatttag gtgaaaattg
     1141 ccgccgtgac atcgtgttgg aattagaaag tctaggcttt gaagtggaag cttcacatca
     1201 tgaagtagcg ccaggacagc atgaaattga ctttaaatat gcagatgtgg tagatgcttg
     1261 tgataatatc caaaccttca agctcgttgt caaaacgatt gcacgcaagc atggacttca
     1321 cgcgactttc atgccgaaac cgttatttgg gatcaatggt tctggtatgc attgcaatat
     1381 gtctttgttc aatgatgaag gaaatgtttt ctatgataaa gacggtgaat tggaactaag
     1441 tgaaacagct tatcatttct taggtggttt gctgaaacat gctcgtgcct acacagccgt
     1501 ttgcaatcca acagtcaatt cttataaacg tctcgttcca ggttatgaag cgcctgttta
     1561 tgtcgcatgg agtggccgta atcgttcgcc attagtacgt gtaccagaat ctcgcggttt
     1621 atccacacgt ttggaattgc ggtcagttga tccatctgca aacccatact tggcaatggc
     1681 agtattgttg caagcaggac tagacggtat ccgtaatcag atcacaccgc cagcagctgt
     1741 tgaccgtaac atttatgtaa tggatgagga agaaagagaa gctgcacata tccaggactt
     1801 accttcaact atccataatg ctatcaaaga attgcgtaag gataatgtaa tgattgacgc
     1861 attaggacaa catatcttct caaattttgt tgaagcaaaa cgtctcgaat gggcggcatt
     1921 cagacaaact gtttccgagt gggaaagaga acaatatcta gaactttatt aaaacaggaa
     1981 taccgtgaat tagaggctgt gaaatcagcg ttttgacgag aaactaacgg aaaaatagtt
     2041 cctcatattt tgccgttttt tgagctaata ccgagggtca gctgattgaa caccgtttag
     2101 tggagaggtt gtgaaaaagc tgccctgcaa caagtaataa gaatagccaa gcaaaaacag
     2161 cttttcttgt ttttgaattg gctgaactta ttaccgcaga tgcaagcttt tgaacaccgt
     2221 ttaccaagag gttttgaaaa agacgtttag tttcgagtat taagaaaaat tttgtgaaaa
     2281 tggctttcta catttttgca taaatttggc ttaataccga gaaactggct tttgaatacc
     2341 gtttatccag aggttgtgag cctaccattt aactccgaat cacaagaaat aatttcaaaa
     2401 acagcttctc atgtttgatg gatattgtga cttgtgaccg aggagttggc aggagaacac
     2461 cgtttattta gaaataagga ggcacgactt ttgtcgcatc tccttatttt ttacttatgt
     2521 gattcgttac cttcaatgaa tccctacaag gcacttttgt tgaaaaaaag gtcatctttg
     2581 aaaaaattag tggaattgtt ttgtttttga tattatttaa tagaaaagat atgccaaaaa
     2641 tccgagaact gtggactacg ttttttacgt aaaggaggta aataagggtg aaagaaaaag
     2701 attgtttcta cgataagcta tataaactgt tgctgcaagt aggaaaaagt agttctttgt
     2761 taggtgggat attatctatc ataatgtttg ttttattttt ttatctatta tttcacttaa
     2821 aatttttcag taacgatttt ctgatttgct taggtttaac gttagctatg tttatatcaa
     2881 gcttattatg tgggttaaat tttttttgca ctcataaagg acttaagaaa taatttctca
     2941 aaggatatcg aaaaagaaaa aattgtaagc ggatagtatt catttgagta cttgactttc
     3001 ccttagtcag cttgtattat acaccttggg caccctttta gagggtcaga agtttacaga
     3061 aatttcggct ccctattctt attgaagaat agggagcttt gtttattgtc tgagactttt
     3121 cctgcacata aattaaaaag gagtttttga acttatgaca aaatatattt ttgtgaccgg
     3181 cggtgtcgtt tcatcaatcg gaaaagggat tgttgcagct tctttaggac gcttattgaa
     3241 aaaccgcggc ttaaaagtaa ctatccaaaa atttgatcca tacatcaacg tggatccggg
     3301 gacgatgagc ccatatcagc acggagaagt attcgtaaca gatgatggtg ctgaaacaga
     3361 tttggatctg ggccactacg aacgttttat tgacatcaat ttgaacaaat actccaacgt
     3421 gacgacagga aaaatctatt ctgaagtttt gcgtaaagaa cgtaagggcg aatacttggg
     3481 agcaactgtt caagttattc ctcatattac aaatgagatc aaagaaaaaa tcatgcgagc
     3541 agctaaaatg actgattcag atgtgatcat tacagaagta gggggaacag taggggatat
     3601 agaatctttg ccattccttg aagcacttcg tcaaatgaaa gcagatgtcg gtacagataa
     3661 tgttatgtat atccatacaa cattgattcc ttacttgaaa gctgctggag aaatgaaaac
     3721 taaaccaacg cagcacagtg taaaagagct acgtggttta ggtatccaac cgaatatttt
     3781 ggtggtacgt acagagcaac ctgtttctca aagcgtgaaa aacaaattgg cacaattttg
     3841 tgatgtcgaa ccagaagcag ttatcgagtc gccagatgta gatacacttt actccatccc
     3901 attagtttta caagcacaag gaatggatca aattgtttgc gaccatttaa aactagatac
     3961 accagaagca gacatgacag aatggaaaga attagaagaa cgtgtcttga acttgaagaa
     4021 aaaagtacgg attgcgcttg tcggtaaata tgtcgagtta ccagatgcat atatctcagt
     4081 agttgaagca ttgaaacatt ctggtttcgc ctataattca gacatcgaga tcgattggat
     4141 caaagcacaa gaattaacga gagaaaatgt agaagaacgt ttgaaagacg cagatggaat
     4201 cttggttcct ggtggatttg gtgatcgcgg agtagaaggg aaaatcgaag cgattcgtta
     4261 tgctcgcgaa aatgacgttc cgtttttagg tatttgtctt ggtatgcaga tggcttgtgt
     4321 cgaatttgca cgtaacgtcg ttggattaga agatgcgtca tcagctgaaa cgaatccaga
     4381 tacagcaaac aatattattg atttaatggc tgatcaggaa aacatcgaga atttgggtgg
     4441 aactttacgc cttggattat acccatgtaa aatcaaaaaa ggaacgaaga cggctgaagc
     4501 atatgatgga gccgatgttg tgcaagaacg tcaccgtcac cgctatgagt tcaataacaa
     4561 atatcgtcaa ttgtttgaag aacaagggct agtcttttca ggtgtctcac cagataaccg
     4621 tttagtagaa atcgtagagc tgccagagaa aaaattcttt gtgggatgcc aattccaccc
     4681 agaattgatt tctcgtccaa atcgcccgca aaaattaatc aaagcatttg tcggagcatc
     4741 attagcagat cacgaaacaa aataattttt gaacttgata gaaagagaaa agtagcaaga
     4801 gaaggggata agcaattttc ttgtcacttt tctctttttc tgatttgtta ttatttgtag
     4861 gaaaaaagat agaaagcaaa agactttttt cgattaaagc gctattggat attttatttt
     4921 ttgcaaaaaa cttcttgaaa attttcattt tcattgattt tagtagaaaa ctaccgaaat
     4981 gtttggtaaa atagggtcgt tggttaagaa ttgtcttaac aaatttagtt ttacaaaact
     5041 taggaggaat tttattatgc cagtagtatc aggagctgaa tttttaaaag ctgctcgtaa
     5101 aggcggttac gctgtcggcg gattcaacac aaacaacttg gaatggactc aagcaatcct
     5161 tgaagcggct gaagcaaaac aagcaccagt acttatccaa acatcaatgg gtgcagctaa
     5221 atacatgggt ggttacaaaa tctgtaaaga tttaatcact gatttagttg aagcaatgaa
     5281 catcactgtt ccagttgcta tccacttaga ccatggtgat tacgaagctg ctttggaatg
     5341 tatcgaatta ggctatactt cagtaatgtt cgatggttca catctaccat ttgaagaaaa
     5401 cttgaaatta gctaaagacg ttgttgaaaa agcacatgca aaaggtatct cagttgaatg
     5461 tgaagttggt tcaatcggcg gagaagaaga cggaatcatc ggtaacggcg aattagctga
     5521 tattgaagaa tgcaaacaaa tggttgctac aggtatcgac tacttagctt gcggtatcgg
     5581 taacatccat ggtcaatatc cagaaaactg gaaaggatta gcttttgacc acttacaagc
     5641 aattgctgat gctgtaggtc cagacatgcc tttagtatta catggcggat caggtattcc
     5701 tcaagatcaa atcgaaaaag ctatctcaat gggtatctca aaagtaaatg ttaacactga
     5761 attccaatta tcatttgctg cagctactcg taaatatatc gaagctggta aagataaaga
     5821 aggtaaagga ttcgaccctc gtaaattatt agctccaggt aaagcagcaa tcgttgaaga
     5881 tgctaaatct catatcgact ggttcggttc agctaacaaa gcgtaagcat ccacataaca
     5941 gatacgatat ataggaatgg agaacgactt ttgaagtcgc tctccattct tttttactta
     6001 aaacggcaga ccatgctcgt ttcttttttt tatctatgat aaaatacaag catatccatg
     6061 aaaaaagatt aataaacaag gtggaaacag atgaaaaaaa tcgtaatcaa aggaaaccga
     6121 cctttagctg gtgaagtaac gatcagcggt gcaaaaaata gtgcagtcgc attgattcca
     6181 gctgcaatct tagctgattc cccagtgatc ttggaaggtg tgccggacat acaagatgtt
     6241 cattcattaa ttgaaatttt agagatcatg ggcgcaactg tccatttttc caacaacata
     6301 ctagaaatcg atcctcgaga agtcgtatcg attccgatgc cgcacggaaa aatcaatagt
     6361 ttgcgtgctt cttattattt tatgggaaca ctgcttggta aatttggcga agcggtagta
     6421 ggcctgccag gtggctgtta tttaggccct agaccgattg acctacacgt caaaggattt
     6481 gaagctctag gtgctgaagt gacaaatgaa catggtgcca tgtatttacg gacagaaaat
     6541 aaaggattgc gtggcaatcg gattttcatg gacgtggttt cagtcggagc aacgatcaat
     6601 gtgatgttag ctgctgtaaa agcaaaagga cagacagtaa ttgaaaacgc tgcacgagaa
     6661 ccggaaatca tagatgtggc aactttactg aataatatgg gggcaaaagt tcgaggagcg
     6721 ggtacagatg tgatccgtat cgaaggagtc gaaacattac atggctgtcg gcatttcatg
     6781 attcctgacc gcatcgaagc aggaacctat ctagcattag cagctgcagt aggaaatggt
     6841 atcaaagtga aaaatgtgat ctttgaacat ttggaaagct ttatagcaaa attgcaggaa
     6901 attggtgtcc atatgaaaat cagcgaagat gaaatcgaag tttacccctc gaaagaatta
     6961 aaacccgcaa acatcatgac ctatccttat ccaggatttg ccacagatct gcagcagcca
     7021 cttactgcgt tactattgat gactacaggc acttcagaaa tcatcgatac gatctatgcg
     7081 aaacgtgtga accatgtgcc ggaactagca cgaatgggcg ctgatattac cgtggaagga
     7141 aatatgatca tcgtgaatgg tccaaataaa cttcacggca cagaagtcgt ggcttctgat
     7201 cttcgggcag gtgcttgttt agtgattgct ggactattgg cagaagggac aacgacgatt
     7261 tacaatgtag attatatctt acgcggttat gatcatatta tcgagaaact aacagcctta
     7321 ggggcatcaa ttgaaatgat agaggaagtg ggagctgaat gagcgattat ttgacaatgg
     7381 cagaactaga gaacaaaacg ttaaaagaag tttatagtta tgccaaagaa tttaaaattc
     7441 cttattacag taagatgaat aaaaaggaat tatctctagc tgtgattcgt gcacaagcag
     7501 agaaacaagg attttattat atggaaggaa tattggatat cgttggtcaa gatggttacg
     7561 gttttttacg gccaatcaat tatggaccaa gtgcagaaga tatctatatt tctgcctcac
     7621 aaatcagacg attcagtttg agaaatgggg acaaagtggc aggaaaagcc cgtccaccta
     7681 aagaatcgga acgttattat ggtttgatgc acgtggaaag cgttaatgga aaagatccgg
     7741 aagaagcaaa agaacgtccg cattttccag ccttaacacc tctttatccg caaagacagc
     7801 ttcaactgga aacaacaccg caaagactat ctacccgaat gatcgatatc ttttctccaa
     7861 tcggttttgg tcagcgtgga ttgatcgtcg caccgccaaa agcagggaaa acttctgtat
     7921 taaaagaaat cgcaaatggt atcaccgaaa actatccgga agtagaatta atcgttctac
     7981 tgattgatga acgaccggaa gaagtaacgg atctagaacg aagcgtgaaa ggcgacgtgg
     8041 tatcttctac atttgatctg cagccgcaaa accatactcg agtttctgaa ttagtattgg
     8101 aacgcgcaat gcgtttagtc gaagataaac gagatgtcgt tatcttgatg gatagtatta
     8161 ctagacttgc tcgggcctat aacttagtcg ttccaccaag cggacgtaca ttgagtggcg
     8221 ggattgaccc agccgcattg tataaaccta agaaattttt cggggcagca agaaacatcg
     8281 aagaaggtgg cagtttgacg attttagcga ccgctttagt cgatacagga agccgtatgg
     8341 atgacgtgat ctatgaagaa ttcaaaggaa ctggaaatct agaattacaa ctttcacgtg
     8401 aattatctga acgtcggatc ttcccagcca tcgatatcaa aaaatcaggt acccgaaaag
     8461 aagaattatt gatgccaagt gaagaattag aagaaatttg gaaacttcga aaaaatatga
     8521 ctggagattc attagaatat acggaacaac tgatccgctt tttgcgtaaa acaaaaaaca
     8581 atcagcaatt tttcaaagaa tttcaagatg tctcctttgg taaaaaaaat ccgaccagaa
     8641 atctgaaaag ataattgcaa ccttgactga aacatgttaa gatgttaatg ttgtaaatcg
     8701 acaaatcaac tctggttcag caaatggagc agggcaaagg agcgaaaaaa tcatgaaaga
     8761 aaacatccat ccagattacc acccagtagt attcatggat tcaacaactg gtttcaaatt
     8821 cttgtcaggt tcaacaaaaa cttcacaaga aacagttgaa tgggaagacg gtaatacata
     8881 cccagtcatc cgtgtcgaag taacttctga ctcacatcca ttctacactg gacgtcaaaa
     8941 attcacacaa gcagacggac gtgtggaccg tttcaacaaa aaatacggtc tcaaagacgc
     9001 aaacgctgcg gaataataaa aacgctgata actcaacgtt tagcagacta tctgataggg
     9061 atagtctgtt tttttatgtt ttttgccaat ttaaaacttc gtttgatgct gaaaaagtcg
     9121 taggatcata atgcacaaaa caatgaaaaa ctagtatata gttttaatta tccacttgat
     9181 aaaaacacct tatattaacg aaatgaagag gggttatttc gaagtaaaaa accatttacc
     9241 taaaattaga agctaagctt tcctttcaag gaaggctttt tgttttgtta tagacttgcg
     9301 attgtttgtg agctagatcg ttcattttaa tgacttatac acgcggatga tgggtctttt
     9361 tcctctgttc attcataata ttgaacggta cagctagcga tgctatactg acctcagttc
     9421 caagaaaacg ttaacatttt ttgtggggga gaggtttttt atgaggtttg atcaattgat
     9481 cgcagaagaa ctgattgttt tcgatccatc gatagattca aaaggatcat tatttgagcg
     9541 aataggggat gtgttgaaaa aaacaaataa ggtaagaaat gttcaaaaag tcgttaaggc
     9601 actattcaaa cgagaggagg agatctccac aggaatagaa gatggatttg ggattcccca
     9661 tgcaaaaagc aaatatgtga caaaaccagc actgtgtttt tttcatacag ccaaattaac
     9721 ggactatagc gggatagatg aaatgccaat tgaatatatt tttgccatcg tagttcctga
     9781 aaaatcagca gatatgcatc tagagattct cagcacactc tccagacgat tgatgaatgc
     9841 agattttaga aagggaataa aagaagccac tacgccagca gatatcatca gacagattag
     9901 tcaagaatga aaatagtaga gtctttgtcc gagtatcgat agtgaagaaa aaggaggaaa
     9961 ataatcatga aaatcgttgg tgtaactgct tgcccgactg gaattgctca tacttatatg
    10021 tcggcagaaa aattagaagt aacagcaaga aatctaaacc atcaagcaaa attcgaaaca
    10081 caaggtgtaa aaacagaaaa tcagttatct gaacaagaaa tcaaagaggc agatgcaatc
    10141 attcttgcag tagataaaga aatcgaattg gaccgatttg ccgggaaaaa agtaaagcgt
    10201 gtctctacaa gccgagcaat caaagaaccg caagtagtaa tagacgaagc attgagagat
    10261 attggcgtat tcgttgtgtc taatgaaaaa gaaccagctg caaatgagaa aaagccgaca
    10321 atctataatc atttcatgaa tggtgtgaat tacatgcttc catttgtgat cgcaggcgga
    10381 atcatcatcg cactgagttt tgcctttggg attacagcgg ctgatcccaa ctcagcagac
    10441 tataatgtgt tagcagctgc attttcaagg atcggaggag atacagcgtt tgccatgatg
    10501 gttcctgcgt taggcgcggg gattgctaca tcgattgctg gaaaagcagg ttttgcccca
    10561 ggaattgttg ccggattact ggcctctaca ggtggttcag gttttcttgg cggaatgatt
    10621 ggtggcgtgc tagcagggta tatctgtgat tttctggcaa ataaaattag cgtaaaaaaa
    10681 gaattttctg ctatttatca gctgattgtc gtcccctttt tatcgatctt aattattggg
    10741 cttttgatgg tatttgtgat tgaacaaccg atcacatggg tgttggatgc tttgacaaat
    10801 tggctgaata gcttgggaaa tacatccggg ctattgttcg gattacttat cgggatcatg
    10861 atggcagctg atatgggtgg gccgatcaat aaatcgactt cgactttctc gatcggtttg
    10921 atgtcagctg gagtaaatgc accgattgct gcttgtatgg ctgcaggaat gacacctccc
    10981 ctaggtattg ctttagccac tttattattc aaacataaat ttaccaaaga agaacggact
    11041 tcaggtcaat catgctggat tttaggcgct tcgtatatta cagaaggtgc tataccgttt
    11101 gcggtatctg acccgatccg cgtgatacct agtttgatga caggttcagc agtcgctgca
    11161 gggatttcca tggcagtagg agttacttca atggcacctc atggagggat ttgggtgatg
    11221 ctaattccaa atgttatgaa caatttacct atgtatgtct tggcaattgc atcaggtaca
    11281 gtcactacag cctttatgct tggactattg aaaaaaacag tgaaagagaa aaaacaagac
    11341 aagtggacga aaaatgaaaa tctaacaaat gaggtggaag cgtaatgtta tatacaatga
    11401 aagagttatt gaaagtggct aaagagaatc aatttgcagt accagccttc aatatttgca
    11461 gctatgatat gatgaaagca atcatcgaag aagcagagag attagaagca ccagtaatct
    11521 tagaaattca tcctgatgaa atcgcctatt taagtgatga gtttgttgcg gcagtaagag
    11581 cctatgctta tacaagtaaa ctgccgatcg ttatccactt ggatcatggc ggtacgatag
    11641 aagatgtctt acgggcgatt cgaaacggct attcttccgt gatgatcgat gcgtctttga
    11701 aatcattcga cgacaatgta cagattacga aaaaagtggt tgcgttagct catgcggtaa
    11761 atgtctcagt ggaggctgag cttggaacga ttgggaataa tggttctgct gaaggaggtg
    11821 ctgaaacaat catttataca gatcctgatc aagcgaagat ttttgtgaaa gaaacaggaa
    11881 tcgatacact tgctgtagca attggaacag cgcatggctt gtatccgaaa aacagaactc
    11941 caaaattaaa tatcgactta ctgaaagaat tgaaccaaca aatcgatctt ccatttgttt
    12001 tacatggcgg ttcaggtaat ccggacgcag aagtcagcga agcagtaaaa tacggagttg
    12061 ccaaagtgaa tttgagctca gatcttaaaa gcgtcttttt caatgctttg agagaaatat
    12121 tgagtgaaaa tccatcgatg tacgagccga atgcaattta tccagcagct aataaaaagg
    12181 tacaagaagt ggtcaaacat aaaatggaaa ttttgaacac agtaggcaag gctagtttat
    12241 actaaaaata aaaatgtgtg gtcaagaggg tatgacaata gtcttgtcat accctcttaa
    12301 ccacacacga tgttcagaag tagttttttc gaaaaatttc ttcttatttc tcgaagccaa
    12361 tcacttctgt cacaacctct tttttcctgt tactatataa agtaaatggg gtgattttat
    12421 gttgaatgaa gtggagaata gacagcagga gattgtgaat tatctaaaaa acaaccaatt
    12481 tgcaagagtc accgatttga tcgagttagt aaattatagc gaagccacga ttaaacgtga
    12541 ccttgttttt ctggaaaaaa gtggattgat ccgtagagtt cgcggtggcg ccatgcttat
    12601 tgacaatcga aaaattgacg tgccttacct gatgaagatc accaaattag aagaagaaac
    12661 agaaaaacgt aagattgcag atacagcttc ttctttgatt cgagatgata tggtcttatt
    12721 cttggattcc agtacaacct ctttacactt agtcagaaac ctagcaagat ttgatggttt
    12781 gcagatcatt acgaatggca tcgtcacagc tgctatgttg agtgaatata caacggcaaa
    12841 agtatctgta ttgggagggg caattgttcc gaaacgagca acaatcaacg gttcaaaagc
    12901 ctataacgat gcattgtcat attatgcgga tttagcgata atgagttgtc gcgggctaga
    12961 tttcagccaa ggagtaacag aaactcatga aggagaggca atggtcaaaa aagcttttag
    13021 aaaacaatca gaccggcttg ccgttttagt tacacagcaa aaaattgcac ataaattcat
    13081 gcatcaagcc attgcttttc atgaaatcga ttatctggtg acaagtaaag aactgacaaa
    13141 agaacagtat gcgaaagtaa aggaacacca tatcgaatgt ctgtacggat aaattattac
    13201 gtgaatgttc taagtggctt cctcattttt agataggacc ttatctttgc tcgcttctct
    13261 cagccgatgg tataataatg aaaaggttat cttgttagat aatcttactt ttttatccag
    13321 gagaaaggag tgcagaaaat gaaacatgtg aaggcgttgg tagtgaaggc aatcatgatc
    13381 tgggcgattc tgtgggtcgt gctgacaggt ttatacggtg ttagctttat ggattcaacg
    13441 attgttgggg taattattgt agtcatgatc tatgtgctag gggatctgtt gatcttacgt
    13501 aaagtaggga acatcgcagc gacgatcgca gatgctggtt cagctgcagt tatcttgtgg
    13561 ctctatctat attttatgaa tgatacagtc gatatttgga tgaaagtatt gattcctgct
    13621 ctattgatcg gtgtggcaga atggttcttc cacaagtggt tgctaagtca aggcatcgta
    13681 ccagatgaac ggaaaatgga ataaatcaac agttaaacta ttcttatcga taaatgaaag
    13741 ctttaaaaat aaatagatgt ttaattaaga ataatcataa actattttta ctcaaaaaac
    13801 cgctcagatt attttcagat ctgaacggtt ttttttatct attcatcaag atcaaatttc
    13861 atttttcaat gattcaatca attcgccctc tgcttcatga ctatgttttc ggccattaac
    13921 aagatagagt gctgtacata tcaatggaat gactccggca atcacataca atcctctaaa
    13981 tgaagtaaga gattttagca tccccatcaa ataaggacca acgcctaagc ctaggtctaa
    14041 accaataaag taggtagata aagcaatact gattcgatgt tcgcggacga tttttaaaca
    14101 aacagcctgt ccatttgaca taaacgtacc ataacctagg ccgactaatg ctccagagat
    14161 aagcaatgtc acactattcg tcgttacact cagtaaaaat aatcctgctg ttaagaataa
    14221 atagctagga tacatcacgt attgttctcc tttgacatcg aaaatccttc cagtcaatgg
    14281 acgtgtaaat gtgatgacca aagcatatac gacgaagaaa aatgacccag cagcaactaa
    14341 gtggattact tttgtataag aagataaaaa tgataaaaca cttgaataag aaagacccat
    14401 caaaaaggca attcctgtaa tgaaaagtgc tttcttttca ataaaactat tgatattcca
    14461 tgagcgaaga gatatttttt gttcttcaga taatggaata ttttttacag gaaagacaag
    14521 gcaggcaatc gtaacaaaga agaccagtac aattgaaaac cagataatga aacgaaaatc
    14581 agtcatattc agcaaaatca ttcctaaaaa tggcccaaca gcggcagcta aactagtact
    14641 taatccataa taattgatgc cttccccttt tttagatgca gggatatatg cagtaacgat
    14701 agcatttgta gctgtagaaa ctgtaccgta gccgaatcca ttcaagaatc gaactaaata
    14761 caatgccccg atagttggcg tataaagata tgctaatgtt gaaaggagat agaagatcgc
    14821 gccgcctctt aaggtaaact tccgaccaaa tagttctaac tgtttcccca tgaataagcg
    14881 tgccagcaat gttccgatga tatagatccc agaagcaaaa cctgcttggc tcaagctggc
    14941 gtgtaaggtg tcttgcgcaa tgactgccat gatgaccatc aacagataat aaatcaggta
    15001 gacaataaaa ttgatcaatg tgattccaat aaaatattta ttaaataatt tcttttccat
    15061 gtatgttata cattcccctt aagctgaata tgcttttaat ttttgtattt gaacttctac
    15121 atcgtgataa ttggatggat agcgatcaac aagttggcga ataaataaat ctcttgtaga
    15181 cataaacaga tttaaaaatt cttcgcattc gtttgttgga ttctcatcca ctgctttgat
    15241 caattcgatc gagttttgat acgcatcatt taaaaagact aaagaagctt tcatatcaaa
    15301 tcttgtttga tatgctgttt ctaatcgatt aatgattgtg ttcatccagt aattcatgtt
    15361 ttttccctct attcttaaaa aattttactt ctgcagtata acgaataaaa aaaaggaaaa
    15421 aaatcgcata agtgatggaa aaaggtactt aacaaatgtt aatatcatta gtaaaatacc
    15481 tttggacttt aaaaagtttt aaagtactgt atgatctttt tagatgaaag tttggatggg
    15541 ggagcatcat tggatagtag agtcttaaag gaatatttgg ataaaaatga ttttccgatt
    15601 atacgcaaag gatatcataa atatttgaca ttcgaaggga tggaagattc ccatacgtat
    15661 atcttgaaag agggaacggt aaaagcaagt gttatttcaa atgatgggag agaatttaac
    15721 cttcgctata tcaaagatct tgaaatcgtc tctcttttaa aagacgaata ttcacaattc
    15781 atcgattcgc cgtataatat tcgagttgaa tcagaatatg cagagtttta tcgaatcaat
    15841 cgtgtgcgct tttggcaaaa tatcaaccaa gacccaaagc tacaacacta tgtaaaagag
    15901 tattaccgct atcgtctgct ttattcgatg aaaaaaatgc agcaaatgct attaaacgga
    15961 aagctgggag ctgtttgtac gcaactttac gaattatatg atttattcgg tgtgcggaca
    16021 gaagaaggga tgctgatcga tttttcgatc acgaatgaag aaatcgccaa attttgtggc
    16081 atctcaactg ctagcagtgt aaacagaatt ttaaaacaac tgaaaaaaga tggtattatc
    16141 accgttaaag accatcatat cgttattttg aatatggact tgttagaaga caatattatc
    16201 ttttgatgaa caaagaggtt gtgaaatcag cgttttgacc tgagtattag agaaacaaac
    16261 ggaaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt cagctgattg
    16321 aacaccgttt accaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggaacaatt
    16381 tcaaaaaaca gcttttcatg tttgatgaat attgtgactt gtgaccgagg agttggtagg
    16441 agaacaccgt ttaccaagag gttgtgaaaa agctgttctg catcaagtaa taagaagagc
    16501 caagtaaaaa cagtctctta tgtttttaac ttggctggac ttatcaccgt agatgtaagc
    16561 ttttgaacgc cgtttatttg caacaaaagg ggctgtgaca cgatttttgt cacagccctt
    16621 tttttgcgaa cattcattta gcaattttta gaaacttctg ccgccaccgc caaaagtccc
    16681 gccaccgcta gaatgggtag tacttccgcc accgccgctt ccaggaggtg ggcttttggg
    16741 gattcttctt gtcgtgacaa aagaatttgt cagacgatcg gttttttcag ttaatttgat
    16801 actggatttt tctcggaatg gatatttgta gatgcctgat ttcaattgat aacgcgaaac
    16861 agtcacaaaa taaaagacaa gactaaggat aagagccaaa acaacagcaa tgatgatttc
    16921 tactgtcgtc aaaaccttat agcgtgtaat tttgccagtt tcttcgtcta cacgataatg
    16981 tcctcctgga actccgtctt ccacatatgt tgctgattta gacaggtaag cttttgcagc
    17041 attgtaatag gcagcatttg tcatctgatc ataaacatca tctaagatcg aatcgattct
    17101 gctatcagtc aaataatcga tcatattacc agaagtcgaa atgtagattt cacgctgtcc
    17161 catatctaat agcaatactg agccattttg atcatttccg actgcgtctc ttaagtaatt
    17221 atcagcaaag tctcttggtt cttctgaatt agatgtagtc gttacgatca agactcgtcc
    17281 tttgattttt tcattcagct tttctgcctg ttgttccaaa gattggattt gatctgttgt
    17341 gaataaccca gcctcatcat ttaccgtagc tgtatctgca tgagtagcca ccggccagcc
    17401 aacacttact agcaaaagaa agaataaaaa aaattttttc atcatatcaa gtaacctccg
    17461 atcagaaaga ggatcgctag cccagcgaaa atcccaagag cggtcagtcc caattttttc
    17521 tggctgatcg gcaggacacc gctgaccttt cctgtttgtc cattcattgc gtagtagtag
    17581 acttttttac ttgagtcatt gcttcgataa gtaacaagcc ataccggcag taatacgtaa
    17641 ttatttactt cgtttgtgat tgaagcagaa gttcgcacac cggtcaatgt tgtatatcca
    17701 tttgcggtat cttttaacag cttctctgaa tagtctttta attcagattg aatctgtgat
    17761 ttgattgcct catattcgat atctcttttt tctgcttgga acccggctaa atactggctt
    17821 ttaaaatcaa ttgctttatc taacgggaaa ggttgcacgc cttcgaccat cttttgttgc
    17881 gtattttttg ataaggcatt ttttactagt tctttaaaag aaagcttgcc tgctcttgaa
    17941 acagcaaatt gcttcgtttc tgtgtattcg atgtcaccta tccgccatat gcggataact
    18001 gtaccattcg cctgcatggc accatctact tcagcatctg tagcccaata agggaaatac
    18061 acacctgtca acttatcgat ttgatcctta ttgaaaaaag ctttgggaac aaaccatttt
    18121 ttcttggtcc aagcgagaaa tctgttgatt gcttcttctt tctcgacagc aaaaggaagt
    18181 actttatttg gcaagaattc tccgcttaaa cgtcccgata gtacgacggg attatggcag
    18241 tagtaacaat aagtcgcagc agttgtcgca tcagtcacga tctctgcacc acagttggga
    18301 caaagaaaaa gatctatcgc tgcggtatta tctgcagatt cgtccgctgt atctgaaaaa
    18361 ccgcctgcct cgtcaaccgc tttgcgctcg gcttcattca tatcggcaag ctgttcttgg
    18421 gctgtaaaag taaaatcttc ggtaggtgaa ggttcttctt tgctttcttc gccagccata
    18481 cgcgcttctt tttgcttttg ctcatattgg ctgacttctt cttctgtata gatattcagg
    18541 cagtattcgc aatgaaattt ttggtctttg ggatcaaaag tcaacggacc gccgcaatta
    18601 ggacatttat gtgtaagaac atccatcagc tgcacacctt tctaaataaa ttaattaacg
    18661 gggattcctt taaacggttt accacagttt ggacagaatt tagggatgcc gttggaaaga
    18721 tccacgatct cattgcattc actgcatcgc atttgtagct taggctgaac gttttctaca
    18781 ggtttggcag ctccgcagtt ggaacagaat tttccggaat tttctgtccc gcaaactgga
    18841 catgtccaag tatccgcatt tccttgtgca gtttgttgat tttgtttttc tgcttgttgt
    18901 ttgatttgtt cttggttatt ttgagctgct tgagaaagat aagaaccatt tgcattcatt
    18961 cccataccta cacccataaa gccggtcatt gctcctgctt cattttttcc ggctgcttcc
    19021 ataccacgtg caatggaacc ttgcacgtat ccttcacgta cgctaggatc acctagcatc
    19081 gccccttcgt tacgcatatt gatcagtttc acagaatcat cggtgtaaga aatgctggct
    19141 acagccacag aaacaatctc catcccacgt aattcacgcc aagaatcatc caatgcagta
    19201 tccatgtatt tgcttaattc caaactctta gatggtacat aagaaatccg ttgaccatct
    19261 gcagacatct gattgattgc tgattgtagc gctgttagga attcagctaa atactgctca
    19321 ttgatatcat tgatttccac ttgtgttttg tttttaggga ttgcgtttgt ataaaataaa
    19381 atcgggtcaa cgacatgaat agaataagtg ccatgtgccc gtaagaataa ttctgcgtta
    19441 tagaaattgt caaagtagtt caacggcgcg cttgtaccga atttgattcc ctttatttct
    19501 tggagattga tataaaaaac ttgttgtttc tgcggtgtga cgccgccaaa tttgaatcgg
    19561 tcaaaagttt cagcaattgc ttctttcagt gttccattga acatggaagg agctgcgtca
    19621 tttttaatgg tgtaataacc ttcctcagcg gtataatcga tgatttttcc accatcgacc
    19681 aaaagcatca tcatgtttgg atagacatgg acgactgttc cgtctgttaa aacatcttct
    19741 gttcctttac gattggagcc acgtttgtca tctttgcgga ctttgacacc ttttgtcatg
    19801 accgttgtat cactcatatt gtctggttcg atgacttcta gccattgatc tgccagaccg
    19861 ccgccaacca tacttgttgc tgctttgata agacccataa aaaattcctc ctctgattca
    19921 gctagtgcca aacaattatt gtttatttat tataccatag gaagatgtga aacttctcgg
    19981 actagagacg gaattgaaat ggaaaattgc gtattgttta aatacctgaa aaagccgaga
    20041 aggatagtag tttttttacc taatatcacg tataataaga gatagtgaat tttttaaaaa
    20101 agggggatcc ttatggcata tcaagcactt tatcgagtct ggcgttcgca gcgtttcgaa
    20161 gacatcgttg gacaaaaagc tgtgacacag acactaaaaa atgctattgt ttcccacaaa
    20221 acctcacatg cctacttgtt tacaggaccc agaggaaccg gaaagacgag tgcagcaaag
    20281 attttcgcaa aagcaatcaa ctgtccgaac agtaaagacg gtgagccatg caacgaatgc
    20341 gaaatgtgtc gatcaattac tgctggtacg caagaagatg tgatcgaaat cgatgctgcc
    20401 agcaataacg gagtcgaaga aatccgattt atccgtgatc gggcaaatta tgcgcctaca
    20461 aaggcagcgt acaaagtgta cattattgat gaggtacata tgctttcaac aggagctttc
    20521 aatgccttgt tgaaaacatt ggaagagcca aaagaaagtg tcatattcat cttagcaacg
    20581 accgaacctc acaaaatacc agctacgatc atttcacgga ctcagcgatt tgattttaaa
    20641 cggatcaata cggcagatat tgtcgaacat cttgaattta tcttgaaaga atcgactatc
    20701 agctatgaac cagctgcatt gaatgtgatt gcccgttctg cagaaggcgg gatgcgggat
    20761 gcattgagta ttcttgatca ggcaatctcg tttagtgaag gaacaattac tttagatgat
    20821 gccatgcaag tgacaggtag cttaactgac gaaatgatgg acagttactt gagtgcttgc
    20881 gtgaaaaaag aagtaccaga agcattggaa acattgaacc aaattctcgc agccggcaaa
    20941 gaatcacgtc gctttctaga ggatatgtta caatactgcc gtgacttgct gatgtatcag
    21001 caagcaccta acctagttga ggaacaatcg agtacgatca gaagtacatt tacagaattg
    21061 gcacaaactg tttcagcgga tcagctgttt tcaatgataa agattttgag cgatactcaa
    21121 aatgaaattc gttttaccaa tagtgccgct atttatttgg aagtcgctac agtcaaactt
    21181 gcacagctac cgtctgcttc tgttccttca gcagatgtag catttgcaga tgatcagata
    21241 gtaaaacagc tacaacaaga gattaaggaa ctaagaaacg aaatcaagga actacgcagt
    21301 aatacaggaa ttgagaatac aaaagtacag ccggtacagc aaacaaatca taaaaaacag
    21361 acaagtacct atcgtgtacc gacggaacga gtatataaag ttctgcggga agcgacaaga
    21421 cagcatctga accaagtaaa agaagtatgg gaagacttgc tgatgagtct atctgtcact
    21481 cagcgagcaa tgcttaaagc aagcgaacca gttgcagctg gacctacagg actagtgatt
    21541 gcgtttgatt atgagattgt ttgtcaaaga gcagcaaatg acgaagaatt gcagctttcg
    21601 gtacacaaca acttaagccg aatgatcaaa gattatgcgc ctgatcctgt ttatattaca
    21661 agagaaagct ggccagaact tcgaagaaat tatttgacgc aggcacaaga agagggaaca
    21721 gaaaactctt ttgcttctga agactcagaa ggagaaatcg agttgcttcc tcaagaacaa
    21781 gaagtaccag aagaaacact tgtagtaaaa gcagaggaac tgtttggctc cgaagctgtt
    21841 acagtggtag atgattaata aaaaggagta gataattatg atgcgtggaa tgggcaatat
    21901 gcaaggcatg atgaaacaag tacaaaaaat gcaaaaagaa atggcagaag cacaagagct
    21961 gctaaataaa acagagttta caggtaaagc caccaatgaa ttggtcaaag cggtttttac
    22021 aggcgatcgt cgcatgaaag atatccaaat caatgaagaa gtcgttgatc ctgatgatat
    22081 cgagatgctt caggatcttg tcgtaatggc agtcaatgat gcgatcgcac aaattgatgt
    22141 ggaaactgag aaaaaattag ggaaatacgc aaaaggtttg ccaggatttt agttttaaac
    22201 tgaaggagta atgaatatgc aatatccaga accaattgcg aaactgatcg acagctacat
    22261 gaaacttcca ggaatcggtc agaaaacagc gacaagactc gccttttata cgatcgatat
    22321 gaaagatgaa gtagtaaatg aatttgctaa atctttgtta agtgtaaaac gcgatttgca
    22381 tttttgtagt atctgcggaa acatcacaga agaagatccc tgcgaaatat gtagaaatcc
    22441 ttcacgtgat cgaagcgtta tcctagtggt cgaagagccg aaagatgtaa tggcaatgga
    22501 aaaaatgcga gaatatcacg gactttatca tgttctccat ggtgtgttat caccgatgga
    22561 agggacaggt ccagaagaca tcaatatttc ttctctgttg cagcggctgc atgatgcaga
    22621 tgtaaaagaa gtgattatcg caacaaatgc aacaacagag ggagaagcaa cagctatgta
    22681 tctctcacgt ctgatcaaac ccgcaggtat caaagtgact cgtctagcac atggtctgtc
    22741 tgtaggctct gatatcgagt atgcagatga agttacttta ttgaaagctg tcgaaggaag
    22801 acgtgagtta taaatttcta attaagtgtt ttttcagata caatataaaa gcagcacaag
    22861 agatgggggc aacaatgtga acggactatt tattacaatc gaggggcctg atggtgcagg
    22921 aaaaaccagc atcttgaatg aactttaccc tcttttgaaa gaagtagcaa aaacagaaat
    22981 cattcaaaca agagaaccag gaggcattcc gattgctgaa gagatccgag cagtgatcct
    23041 tgatccaaaa aatgatcgga tggatgaacg tacggaagca ctgctttatg cagcagcaag
    23101 aagacaacat ctagtcgaaa aggttctgcc tgctctggca aaagggaaaa tcgttttatg
    23161 cgatagattt gtcgacagtt ctcttgctta tcaaggtgca ggccgacgca tcggcgttac
    23221 cgagattgct cggttgaatg agtttgctac agaaggtact acgccggatt ttacccttta
    23281 tcttgacgta gattcagaca ccggattaag aagaatcaag aaaaacagac aaaatcaaat
    23341 cgaccgttta gattcggaag gtttggaatt ccaccagcgt gtacgtcatg cttacttaaa
    23401 gctggcagaa gagaatccgg aaagaatcca caaagttgat gcaagaaaaa gctttgaaga
    23461 agtgctgcaa acaagttatc atacaattat tgaacagtat ccgcaatttt ttgaaaacta
    23521 ggaaggtgaa cgtttatgaa aattatttta gcgattatcc aagacaaaga cagcaaccgt
    23581 ttatcaaatg aattgattga tgcaaatatc cgtgccacaa aactatcatc gacaggcggc
    23641 ttccttaagg ctggaaacag tacgtttatc gtgggtattg aagatgaccg tgtagaagaa
    23701 gcattggaaa tcatcaaaaa aacgtgtgaa tcaagaaagc aatttgtatc tacacctgtt
    23761 acactggata tttcgatgga tgggggcgtc ccttatccag tagaagtaga agtaggcggt
    23821 gccacagtct tcgtcttacc tgttgaagga ttccatcaat tttaggagga gctgagctat
    23881 gaatcaagaa ataaacttgg ctcaaatgca accgatcgtg tatcagcagc ttcaacggag
    23941 ttttgagcat gggcgtatcg ctcatgctta tctttttgaa ggggagaaag gaacaggaaa
    24001 acatgaagta ggtatatggc ttgcacagca cctgttctgt acccaaatga aagacaatct
    24061 gccttgtgga aaatgcaaca attgtcagcg gatcgaaaag aaagaacatc cagatgtttt
    24121 ggttattgaa ccggaaggac aaaccatcaa agtcgatcag atcagacgtt tgcagacgga
    24181 attcagtcgc agcggttacg aatcaagaaa aaaagttttt ttgatcaaag aagctgaaaa
    24241 aatgaatagc agtgcggcaa acagtttgtt gaaattcttg gaagagcccc ctggagattt
    24301 tttagcggtg ttagaaacag atgcagtagg aagaattctc cctactattc aatctcgttg
    24361 tcaagttctg cattttcaag aactttcgaa agaagcactg atccatcgat tgcaagaaga
    24421 gcagattcca ttggaaaaag caaaactttt ggctttctta acaaacagtt taggaaaagc
    24481 agttgaaatc tctcaagatg aatggtttaa tgatgctaag gacttgattt atcaatggtt
    24541 cgtttacttg caaaagcaag atactcaagc ctttatctat gtccaaaaaa aattggtaaa
    24601 atcatttaaa gaaaaaacac aacagtttac aggattatct gtcttgatgt tttattatca
    24661 ggaagcacag cgtcaagcag tagcaaacgg cgaatatggc aaagtgaaac agataaacca
    24721 gataattgaa cggatattga tcgcagagca gaaactacgc agcaatgtca gttttcaggc
    24781 tgttgcggaa cagttcgttt tgcatacgat tttttagctt gccgcaaagg atgatgatca
    24841 aggaggatgg aaatggtaga agtagtaggt gtccgatata gagatgcagg tcatatctat
    24901 tattttgcac caggagactc tgaatatgtt tatagtgaca aggtattagt cgaatctcag
    24961 caatccttac atattgctac tgttgtcctt cctaaaaaag agattgatcc caatgacctt
    25021 cctgaagcta tccgtccgat cttgcataaa gcgtcacaag ctgaattaga aaaagacaaa
    25081 aaaaacaaaa aagatgctta tgatgcattg aaaatcgcaa aagaaaaagt gcagttgcgt
    25141 gagttggaaa tgaaaatggt caaagccgaa tacacatttg atcgaaataa attgattttt
    25201 tctttcacag cggacgggcg aattgacttt agagaattgg taaaagatct ggccgctgta
    25261 tttcgtactc gtattgagct tcgacaaatt ggtgtccgcg atgaagcaaa gataataggc
    25321 ggaattggtc catgtgggcg tccattatgc tgttcttctt ttttaggcga tttcgtgcct
    25381 gtatcaatca aaatggcaaa agatcaaagt ttatctttga atccaacaaa aatttccggt
    25441 ttatgcggac gcttgatgtg ctgtctgcaa tatgagagcg aagaatacga gacagcgaaa
    25501 aaagaactcc ctgactttgg aaaagtaatt gctacacctg atggtaaagg gaaagtcgta
    25561 gggttgaatc tgcttagccg tatcgttagt attcggctga ctggacggga aaatcctgtc
    25621 gaatatgaat gggatgaatt gaaagaattt atccaagtag gtgagatgaa tggataagag
    25681 atcattatac gacggattga atcaattaga atcagagttg agaaatacat taacagaact
    25741 tgttgaaatg aagcatgctt tgcatgagat cgtagaaaaa aatacaactt tggagatgga
    25801 aaatcaacgt ctgcgggaac acctgcggga attgaaccaa gttgcacaag ctccagctga
    25861 aaatgtgaag caggaacttt ctaagtctcg tatgaatctt gaaaaaattt atgaagaagg
    25921 attccatgtc tgctacgatt tatatggatc aagaagggac aatgacgagc cttgtgcgtt
    25981 ttgtctagaa gtgatctatc gcgaaagcgg tcgatgacca ataacaagag gagactggag
    26041 cggagaaata ctgctccagt ctttttatta aggagattgt gacagatgta taatcaaaaa
    26101 agtttcaagg gaaatccggt atatggggat ttatatcttg taccgacgcc aattggaaat
    26161 ttagaagata tgagctacag aagtgttcgt atgctgcaag aatccgatct tatcgcaagt
    26221 gaggatacgc gtaatacaca aaagttattg aatcattttg aaatccatac accccaaaaa
    26281 agtttacatg aacataatta taaagaacgt gtccctcaat tgattgcttt gttaaaagaa
    26341 ggaaaaacga ttgctcaagt cagtgacgca ggaatgcctt ccatcagcga tcctggacat
    26401 gagctagttt tagcctgcat aaaagaaggg atttctgtga tctctatccc aggacctaca
    26461 gctgggatga cggctttgat tgcttcaggg ctagtgcctc aaccattttt attttatggc
    26521 ttccttccac gtaagaaaaa agaacagaaa gaaacattga aaaagctaaa agaacagaca
    26581 gttacgctga ttttttatga gtcaccttat cggatcactg ctacattgac gaatttctta
    26641 gaaatttttg gaaatcgcca agtcgttttg tgtcgggaat tgacaaaggt ccatgaagaa
    26701 taccttagag gaagtacgga agagctactc gcttatatcg aagaacatcc agtaaagggt
    26761 gaatgttgtc tgcttgtaga agggaatttg ttcgaaatgc cagtgacgga agttgatgaa
    26821 gagttgggaa cgttaaagga acaagtagaa gcaaagatcg cttcaggcga aaaaccaaat
    26881 gctgctatca aagcagtagc gctgaaaaat gggctgaaaa aacaagaagt ctaccgtcag
    26941 taccatgaat tagactgaga tagtataagt tgtctataat gaaagactaa tgaggaaatc
    27001 agctactaac ttttcaaatc tcttgaacgc gaacgaatgt tcttgtataa tgaataagag
    27061 gtgaggatga tggagctgca atttccttta cataatgata catcaagaaa aatcattcat
    27121 attgatatgg atgctttttt cgcttcggtt gaagaacgag atcatcctga gtttcgcgga
    27181 catccattag taattgccag acaccccagt gacactggtg ggcgtggagt agtgactaca
    27241 gcaaattatg aagcaagaaa attcggaatt cattcagcaa tgagtgcaca aaaagcgtat
    27301 gagctttgtc cgcaagccat ctttaagcct ggcgaccatc ataaatacag cgatatttcc
    27361 aaacaagtac gggaagtatt ctatcgttat acagatattg tggaaccagt tagtatcgat
    27421 gaagcgtatc ttgatgtgac cgaaaataaa atcaattgca agtcagccat caaaatcgcc
    27481 cgaatgatcc aagcagatat ttgggagagt gtccagctaa catgttctgc gggagtaagt
    27541 tacaataagt ttttggccaa attagcatca gactatcaaa aacctagagg aatcacggta
    27601 gttgcccctc aagacgctgt gccattttta aaagctttgc caattgaaaa atttcatggg
    27661 gtagggaaga aaactgtacc tagaatgcat gagctaggga tttatacagg aaaagaccta
    27721 tatgaatgca cagaaatgat gttgattcga aattttggaa aaatgggcta ttctctttat
    27781 cgtaaagtca gaggaatcca tgattctcca gtaaacgtta caagagagcg aaaatctgtg
    27841 ggaaaagagc atacttatgg gcagccgttg caaacggaag aagcagtgct gactcagttg
    27901 cgccaactag ctgaaaaagt agaagaagca ctgagaagag tccaaaaaca tgggaaaaca
    27961 gtagttttga aggtcaggta cacggactat tctactgtga ccaaaagagt gacattgcca
    28021 gaatatatct ataaaaaaga agcactcttt tatcaagcaa gccttatatg ggaggaaatc
    28081 ttgggagtag aaaaaggaat ccgattgtta gggattactt tgacgaatct ggacccgatg
    28141 acttatgaaa acatcgtcct tccattatgg gaaaatcaag aaatataaaa acagctttga
    28201 gagttgaaac cggaaatccg ttttctctcc caaagctgtt tttatatcaa gtcttgcttt
    28261 ttgatttgtt cataggtttc tgttgcatcc gtacattttt cccaaatcac aacttgattg
    28321 gattctaaag atttttggac gtcaatgatc cgctggttgc tacttccgcg aaattgtagt
    28381 tttaggtcac gtttgcttaa ttcaaagcgt ccgtcaacaa ggatatcgat ttgcgaaagg
    28441 agttctaatt tatcttctga atcttgtagc aattcatcaa aggtatagcc tgtccatgac
    28501 cagatatctt ttgtcgtacc gaactctttt cggatacgtc gtacgacttg cagacaaaca
    28561 tctgtattca aaaaaggttc gccaccaagt aaagttaatc cttgcacgta atcgttgcgc
    28621 aagtcagcga tgatttgttc ttctaattca ggtgtgaatg gtttgccata tcggaaattt
    28681 tgtgcagctt tgttgaagca gccttcgcag gcaaataagc agccactaac gtaaagactg
    28741 ttgcggacac cttcaccatc gacaaaatta aacggtttgt agtcagcaat atagttttgg
    28801 ctgtagtctt ttgctaacca ttcttttggt tttggatttc tcataaaaaa acttctttca
    28861 tttaaggttc aaagatatag ctattgaaga taaaatctac tcgaccacat aaataagaac
    28921 aaactgacct aaatccattg ttcaagaaag cttgttctta ttttgataga gggcgttcaa
    28981 agatacaaag ccatcgaaag agggtggtga aaaaagactt gtcatagcct cttattctga
    29041 ataaacggtg ttcaaaagct tgcatctgca gtaataagcc caactaaacg aaaaatatga
    29101 ggaactattt ttgtttagct attcttatta cttgatgcag gacagctttt tcacaacctc
    29161 atttcatgtg tttcacacgt gaggaaattt ctttatggcg gccatgtacc atcggacgag
    29221 cttgcggatt tcctaaatag ccacaagtac gtttgactac atcacatgtt tttggatcat
    29281 gatttccaca ttctggacat tcgaaccctc gttctgtcgg tttgaaatca ccttcgaaac
    29341 cacattgata gcaatgatcg attggcgtat tcgtaccaag atacccgatt ttgtcatatg
    29401 caaaatccca tacagcttct aaagctttcg gattttgttg gagcataggg tattcacagt
    29461 agtggatgaa tccacctgaa caatattttg gataatcctt ctcaaattcc aatttttcaa
    29521 atggcgttgg gtttttacgc acatcatagt ggaaactgtt tgtataatat tctttatctg
    29581 tgatattttc gatgatgccg aatttttctg tatccaaacg gcagaaacgg tcagttagac
    29641 tttcacttgg agttgaatag acactgaaat gataaccata ttcatccccc catgcatctg
    29701 cattagcttt taattctttg acgatatcaa gtgtgaaagc ttttgcttct gggtttgttt
    29761 cccattcgcc accgaagaaa gctgaagcaa cttcatacaa tccaatgtag cctaacgata
    29821 cagtagctcg acggtttttg aataattcat tgacagaatc ggtacgtgaa agacgtttcc
    29881 cgaaagcacc atacatatat aaaattggcg catttgctgg aatggcttct ttacaacgtt
    29941 ctacacgata gagaagcgca tctttcatga tttctaagcg gtcagacagt aactgccaga
    30001 atttattctg atctcctttt gattcgatcg cgattcttgg caaattcaat gtaacaacac
    30061 ctaaattcat tcgtccgaca tttacttctt gaccattttc gtctttccac ccttgcaaga
    30121 atgaacgaca gcccataggt actttgaaac ttccagttaa ttcaacgatt ttatcgtaat
    30181 tcagaatatc aggatacatt cttttcgttg cacattccaa tgctagttgt ttgatgtcgt
    30241 aattaggatc ttcagggttt aagttcacac cttttttcaa agtgaagatc agtttaggga
    30301 agatagctgt ccgcttttca ctccctaatc cattgattcg aatctgtaaa attgcttttt
    30361 gaatttctct ttcaaaccag ttttctccta gtccaaatcc taatgaagtg aaaggagttt
    30421 gtccatttga agtaaataag gtattgattt catattccaa actttgcata gcatcgaaga
    30481 tatctttttt ggtttttgct tttgcatatt ctttttggcg ttctggcgaa tcgatccatt
    30541 cttccgcgtc ttttagatgt tttttatagt tcaattctgc aaaaggagca agcaattcat
    30601 ctgttcggtc ggcagaacag ccaccatatt gacttgacgc tacattggca ataatctgtg
    30661 agatttgtgc tgtggcggtc tggatagatt ttggcgattc gacttcggca ttgccgattt
    30721 tgaacccatt gttcagcatg cctttgaaat caatcaagca gcaattcgtc atcggtgtat
    30781 aaggatgata atccaaatca tggtaatgga tatcgccttt ttgatgcgca tttgctatat
    30841 gtggtggcaa cattttcagt ccgattgatt ttccaacgat cccggctgtc agatctcgct
    30901 gtgtattgaa aacattactg tctttatttg cgttttcgtt cacgacagtt tggtccttgt
    30961 tgattagctt ttcaatcgaa aagttgatat ctgttgcttt actgcgagca aaatctcgtt
    31021 tggtacgata attgatgtac tcttctgcta attcatattc gtttttttct agtaatatat
    31081 gttccacgat gttttgaatc tcatagattt tgacattttc agcaaaccgg cttttgattt
    31141 cttggacaac caagtctgca atcatttgaa ttttttcata agtaagtggt gataatgagc
    31201 cgtgaatttt cttttctgct ttgatcagtg cttcattgat cttttgttca tcgaaatcaa
    31261 catggcggcc atcgcgtttg atgactttca tctgggatgt ctctggctgc tgagtaacga
    31321 cagttttttc ttcataattt actttcatta tcttcaactc ctgctaaatt gttcttgtct
    31381 atcataaaac aaaatcgggg aaggagcaac taaatatcgg ctatatatag tattcaatgt
    31441 gctgtataat tggatttcta tactatatgt agtgtatatt ttattgtgaa aaaaggtgaa
    31501 aacttgtagc tggtggattt gttttttgca tatttaattt tgctgaataa aataattgaa
    31561 taaaattttt tattcagtcc aagtaaaagt gacaaaaaca taagagcaaa ttattcgtag
    31621 atatgctact atgcaaataa atagaaatga aaaatgaaag aggaggagaa gaattggaaa
    31681 agatgatcga agtgaaacat ctaagtaaaa gctacggcag caaactttct cccgtcaaag
    31741 ccttagataa tatctcattc acagtagaag aaggcgagtt tgttgggatt atgggcccaa
    31801 gtggtgcagg aaagacaacg ctgatgaata tcttgtccac gatcaatttg cctacgatgg
    31861 gcagtgtctc gatccaagga aaagaaatca caaaaatgaa aaacagtgag ctcagtgact
    31921 ttcgtcgaaa aaaactagga ttcatttttc aagagttcaa tttgatcgat accctaaatg
    31981 caaaagacaa tatcctattg cccctagcag ttgaacggat gactaaagag gaaatggaaa
    32041 agagagtaag acatgtagct caattgttga atatagaaga actgctcaaa cggtatcccg
    32101 atgaactttc tgtcggacag agacaacgaa ttgcagctgc tagagcactt gttgttcagc
    32161 cacaagtgat ttttgcagat gaaccaactg gctctttgga ctcgaagtcg gctacagaat
    32221 tgttgaacta cttgaagacc atgaatcaaa aagaaaaagc aacgatactg ttagtcaccc
    32281 atgatccata tacagcaagc tactgcgata gaattttatt tattaaagat ggtgtgattt
    32341 tttctgaagt tgtcagaaga gggacaagaa gagagttctt tgagaaagta atagacatgc
    32401 aggcgacgat tggcggagga ggaaaaatga atgctgtata aactagccgt taaaagtttt
    32461 tttcggcagt cacgaggcta tcttgtttat tttttcagtt tgactttatc cgtaatgatc
    32521 tattattcct tcagcgcaat gacctacgat caatctttag tcagaagagc aagtcaagat
    32581 acttcgatag atggtggatt gagttttggt ggatttatca ttactgttgt tttgcttttt
    32641 ttcgtattca gtgccaaccg atttttcttg aatagaaggc aaaaggaaat aggcatctat
    32701 cagctttttg gtatgaataa actgcagata tcaggtatat atgtgttaga aatcatgata
    32761 attggattgt tcgcctgtat ttctgggata ttactcggta ttattttttc caaactcttt
    32821 tcaatgattt tggttcgaat gatggatatg gatcttacta gtccattttt tatctccatt
    32881 ccatcagtag tagatacttt atttgctttt ttcatcatcc ttttagcagt atctgtatat
    32941 tcaatatgga aaatatggcg ctatccaatg attcgaagtt ttggagaaaa agaacaagca
    33001 gatagttcaa caatgcgctt caggacaagg caccgattac tcgcagtcat cgggttacta
    33061 cttttaacga gcggatattt tggagctagt cattttaggg aaatcacttc atggatgatc
    33121 agttatcatt atcttgatac attagttttc ttttttcctt ttttaatttt tttcgtttgt
    33181 gtggtcggta cttatttgtt tttctgttat acatttcggt tatttttgat catattgagt
    33241 aaatggaaga tttattacta tggtatcaat tttttgataa tgggtaacac gcaagttcat
    33301 ttgctcaaaa gttggcgtac aaattctctg attacagtca ttctcggaat ttctttaatg
    33361 acaattggcg gcacggctag tatctctact attttatcct accaggagaa agtttcctcc
    33421 ccgatggact atcagtttga tgttgagaca gagaaaaaaa tcaagccgat tctagcagag
    33481 gaagggcaga agataactaa agaaatcacc ttgcattata aagtcattgg gagtatctat
    33541 agtcaaaatc tcgaagaacc tatgtttcaa actgctaatc tgatcacgga aacacaatat
    33601 caagccttca gaaaagttaa tccagatttg ccgaacattt ctttgaaatc acctggtcat
    33661 actgttttgc ttgatcaaat gcaaactctt tttcggaata tttcattata tggatctagt
    33721 attggactgc cagataatca agaattgaag attcaacaaa tgcttccaag tgtattaggt
    33781 gacgaaacaa tggtatatct aagtcctgta ctgattgtag atgataccgt ttttgaaaaa
    33841 gcaaaaggtg tggattatca agtagtcaat gtagatgtat caggtggaaa taatgaaaaa
    33901 atagatgaac gactgagcaa tgacctgtcg ataaagtggc tcaatgctgt ttattattct
    33961 tatgatatcg taaatggaag tctacaagga gaaattagca caaagaaact tccggatgac
    34021 caaatgaagg aagcacaatt tagccaagaa agctctcgtt tgaatttctc ttccagctat
    34081 tcgaaattgc gtgcagttaa tcggcgaatc ggtatcaata tttttgtcac actatttgtc
    34141 gggatgatcg tgattattgc tactggtagt attttgacag tccggcaatt agcagaagca
    34201 gaggaagaaa aagaaaatta tcacctattg actaagctgg ggattccaca aagaaggatc
    34261 agacggatga tcttcgagca aaacgcactt accttctttc cacccatgat attagggatc
    34321 actcacgcag tatttgcaat caatgtcttc actcagtata taaaaactgc cgattactgg
    34381 ttggcttatc ttgtgagtgg cttattgatc cttatctatc ttttgcttta tgtcattacg
    34441 agtcagttat attgtcggat tatcgaggaa tgatatacgg aataagacaa gataataaat
    34501 agaactaaat tttgaatacc atttatttgg aataagaggt tgtgacacga tttttgtcac
    34561 aacctcttta ttttttatat tttttaaaag tacgagaaat tataaagaaa aaagcagtga
    34621 tttgatgtgc tatttttata aaagctatgt gacaaaactg ttagtaaaaa aacggtttct
    34681 gtaagctaga cacatgtttc tttttgtcaa aattagttac actttgtgtg aggttatatg
    34741 aggagggaat ccaatgaaca aatggcgttc taatccaaaa ttgaattatt ggggaaaatt
    34801 catcattcga acaatttttt atagtgcagt actgctgttc ctgatttatc tgtatcacta
    34861 taaaaatatt caaggaggaa cttttatcta taacgaattt taggaggaag agtatggaaa
    34921 tcaaaacgat tattgaagca atcgatgatt ggggaataaa tgaacctgat cgagttgctt
    34981 atcaggcgga tgagacgcat acttttggtg aattgaaaca agcatcagac gcacttgctt
    35041 attatttaga aaaaaaggta gaaggcgatg gaccaattgt cgtttttggc aatttggagt
    35101 ttgaaatgat tgtctcattt ctaggggttg tcaaatctgg ccatgcctat ttgccgattg
    35161 aagagcatac acccaaggag cgtatcctat cgatctttcg ggtggctaaa ccatctatgg
    35221 tcatcagtat cggggactgg atagaagata ttccttcggt tcctgtcata acaaaaaaag
    35281 aatttcaaga aatcaagaag ataccagtag gattttatcc cagaaatagc gtaaaaggta
    35341 cagaaaattt ttatattatt tttacttctg gtactactgg agaaccaaaa ggcgtacaaa
    35401 tcagtcatga taatctagtc agctttgttc aatggacgat ggaagacttt gggatcaagc
    35461 ctggtatgca tttcttagcc caagcgccat tttcttttga tttatcggta ttttctattt
    35521 atcctgcatt agtttctggc ggaatgctga agccgttaaa taaagcagtt gttcaagact
    35581 ttcgtcaatt attcgcgaca ttgccagcct tgaagttgaa tgtgtgggtt tctactccat
    35641 catttatgga tatttgcttg atggaaccta cattcaatgg agaaaatgtg ccggaattgg
    35701 acatgttttt attttgcgga gaagaattga cgaaaaaaac tgcagaaagt ctacttgatc
    35761 gattcccaaa tgcacggata tataatactt acggtccaac agaagcaact gtagctattt
    35821 caagtattca gattgaccag catgtactag atagttatga ccgactaccg attggctacg
    35881 taaaaaaaga cacgaaagtt tctattgtcc aagaaggtaa acctgtttca aaaggagaaa
    35941 cgggtgaaat cattattgca ggaccaagcg tatcaaaagg ttacctgaac aatcccgaaa
    36001 agacagctgc tgcgttcttt tgtcaaaatg acttagcaag ttaccataca ggggatgcag
    36061 gtagactaga tgaatatggg cttttatttt atgaaggacg tatggatcag caggtcaaat
    36121 tacatggata ccggattgaa ctcggggata tcgaacatta tctgctccaa gacaaccgaa
    36181 tcaaacaagc tgtcgtcgta ccaaaatacc aaggaacaaa agtacagcag ctggttgctt
    36241 ttgtcgtatt agaaaacaaa acgaaacagc cggattttca gttgactaaa tctatcaaag
    36301 aacaattact agaagttgta atggattata tgattcctca gaagttccat tatgtcgatc
    36361 tgctgcctca gacagttaat ggaaaaatcg atcgaaaaaa attaacagct gaggtgaatc
    36421 ctgaatgatg ttacctcata tgatccctta tgcagagccg ttttattttt ttcttttagc
    36481 cattgcgctt gtaccgatcg ttctatcttt attgatatgg gaaaaaagat taccagttta
    36541 tcaatcagca gtgacacttt tctttttatt tgtcagcttt ggaggtaatt attggcaaca
    36601 gggagcggcg ctgattgggt atgttttgtg gcagacgata cttgtatggg gctattttgt
    36661 ttatcggcaa aggaaaaatc agacaggatg gttttttgcc gccgttattt tagcgataat
    36721 tccactgttt ttaacgaaag tgactccttt ttttgaacat ggaaaagcat cgttacttgg
    36781 ttttttagga atctcttatt taacctttaa atctgtacaa atgatcatgg aaataagaga
    36841 tggcatgatc aaaacatata agccatttcg ttatatccag tttctgctgt tctttccaac
    36901 aatctcttct ggaccaatcg atcgctatag gagatttgaa aaagatcagc tccatccgcc
    36961 tgttcgtgat aaatatgttg atcttttggg aaaaggcgtc caaaatattt ttgtcggttt
    37021 tttatataaa ttcattatcg gttattattt tggtcaagta ctgcttcctg tagtgggacg
    37081 cattgcctta cagcatggtg gtatttcatg ggcattagta ggttatatgt acgtgtacag
    37141 tatgtatttg ttttttgatt ttgctggcta tagcttattt gcagtcggta caagctacat
    37201 gatgggatat cagacaccaa tcaatttcaa taaacctttt ttatcatgga atatcaaaga
    37261 attctggaat cgttggcata tgacgctgtc cttttggttc cgtgattatg tatatatgcg
    37321 attgatgttc ttcatgatga agaaaaaatt ttttaaaagc aagatcgtaa tgtcaaatat
    37381 cggttatttt gctttgtttc ttctaatggg tatatggcat ggattgacgt ggtactacat
    37441 cgtatatgga ctgtatcatg cttgtctgat ctgtctgaca gatgcatgga tccgatttaa
    37501 gaagaagcat aaagagcagt tgccttctaa caaatttacg catggcttag ccgtattttt
    37561 gacatttcaa gcagtatgtt ttagcttcct gattttttca ggatttttag ataaactttt
    37621 ttttagctaa cgaatagtga catattaatt tatataacaa aggagaaaag gaaaatggaa
    37681 gaacaaattt tatcaatctt agaagaaata acaggaacag atgaagtaag aaaaaatcgt
    37741 gatattgatc tatttgatga agggttgctt gattctttag catctgttca attattagtc
    37801 gagctagacg gagagctgga tatccaagtc cctgtatctg aattcgagcg agaggattgg
    37861 tgcacaccga ataaaatcat tcaacaagca actgcattga agggttgagt atgaaaaaga
    37921 aactttttgc tattttcggc ccggttgttt tagctgttgg cttactgttt gtgtttttta
    37981 cctcctccta tcaagtagat atctctaatc cagaaacgat tagacgtgcc tcttcttcca
    38041 tgtcagtgaa cgtgttgaaa ggagatgcga taaaaaatta tgcattatct gaaaaacaat
    38101 atattccttt ttttggctca agtgaattaa gcagaatcag tccatttcat ccgtctgttc
    38161 tggcagaaaa ataccagcga aactatcgtc catttttgct gggagcaccc gggacacaat
    38221 cgttaagcca atatatgatg atgcgttctg caggagatgc tatgaaaaac aagaaagtcg
    38281 tctttattat ttctcctcag tggttcgtta aaaatggtgt aaaaactgat tactttaata
    38341 cgtattattc tgaacttcaa acatacgact ggttgttttc aatgaagaaa gtaacgcctg
    38401 ctgatcgcta tttagctaga agattattaa ctttctcaaa agtaaaagag aatgatacgt
    38461 tgacggcgat tctgcaaacg atcaaaaaag ggaagctacc tttacctgaa tctttgaatc
    38521 agctgcgcag ccaatggaac atgctgaaga gagaggatga agtattcagc aatattgggt
    38581 tgaaagatcg acagcaaaaa atcgatcatg agtcaaaacg attaccaaaa caataccaag
    38641 aaacagaatt atccatttta gcaaatcaaa ttggagaacg agaaacgacg aataatccat
    38701 tcgggttgaa aaacgatttt tatacgcatc gtatccgagc ccacgagcca gagctcaaac
    38761 agtcacaaaa aaattgggat tatcgttttt cgccagaatt ttctgatttt cagctggtct
    38821 tggatcaatt agcaaaaaac cataacgaag tattgttcat catcccaccg gttaacgaga
    38881 aatggagcga ttataccgga ctatctcaag aaatgttaca aggatttgcc aaaaagatca
    38941 aatttcaatt aaatagccaa ggattcaatc gaatagctga ttttgtcaat caagcgggta
    39001 ccaactattt tatggaagac acgatccatc taggctggaa aggctggttg gctgcagatc
    39061 aacagatcag accgttttta gaagaaaatc atattactgc tagtaagtat catcttgacg
    39121 atgctttttt tagcaaatct tggcaacatc aaataccaga taaactacaa ttaaaataaa
    39181 gaaaacaggc gagtgttgct cgcttgtttt ctttattttt gaatattctg aataaaagat
    39241 aaaaatacca agtttgcctg aacaaaaatc ataagcaatg gtataatcac aatatgaata
    39301 tagaaatggg ggaaaacatt tggatacaaa agaagaacag ctacttattg atttaacttc
    39361 tgcaaaagga gtacctggaa atgaagaaga ggtaagagaa gtattcagag aatatgccaa
    39421 accattcgct gatgatattt tttatgacgg tttaggcagt gtgattgcca aacatggtgc
    39481 tggcggaggc cccaaggtct ttatttctgg acatatggac gaagttggtt ttatggtaac
    39541 aaaaataacc gaaaaaggat tcttggaatt ccaaactctt ggaggttggt gggggcaagt
    39601 catgcttgct cagcaagttg aaatcaaaac acgtgaaggg aaaatcgtcc acggtgtgat
    39661 tggttccaaa ccaccacatg ttttaacacc tcaagtaaga aataaacctt atgaaataaa
    39721 ggatatgttc attgatattg gcgcatcaag ccaagaagaa gccaaagagt ggggcatacg
    39781 tcctggagat atggtcactc catatattga atacaaacga atgaatggca gtaaatattt
    39841 actggcaaaa gcatgggata atcgtatcgg gacagctgtt tctctgcgag tattagagaa
    39901 tctatctaaa gaagcacacc caaatgtttt attcgcagga agcgatgtgc aagaagaagt
    39961 aggcttgcga ggtgctcgaa caagtacaca ccttgtaaat cccgatattg cttttgcttt
    40021 agatactggt acagcaggag acactccagg aatgacacca aaagaagcag attcgatttt
    40081 aggaaaaggc ccacagattt tgatttttga tgcatctatg atcccgcata aaaaattatt
    40141 gaattttgtt attgatgtag cagaggaaat ggatattcca ttccaatata cagtgatttc
    40201 tggcggagga actgatgcag gacaaatgca tttgacaaga gatggtgttc cgtctgtagc
    40261 cattaccgtt cctgttcgtt atctgcattc ccatacatca gtgattcatg aagatgatta
    40321 tttgaataca gtgaaactag tcacagaatt agtgaaacgg cttgatagtg aaacagttga
    40381 aaaaattcgg agttatgaat aatgaataat agaataccta tttctgaata tcggaaaaaa
    40441 gcacgagttt ctttggaaaa tcaatgggga atcaatactt ggcttgtttt cttatctata
    40501 atatttacga gcgtcatcca gaactttttt gacggtttca gtccttttcc ggaagaaagc
    40561 atgcagcagc acttattgag ttttctagtc gataatttac ttttattcgc attcaactat
    40621 gcattgtttt acattgcgct agtagttgta cggggtggca gaccaaaaag taacctttta
    40681 ttttcagttt tccaaagaaa gtattttggt tcgatacttg gcatcaattt gatcaattca
    40741 gtcatcaact ggctactcaa cagtttaatc tttctgccca tgtttttaat cagcggagtg
    40801 agtacttata tgcagctgct tttcagttca aatgcggtac ctacgggtgt catgccagaa
    40861 gattttcttg atatcagctt tgttttatcg atgataggga tgatttttgt ttcagcactc
    40921 atcatgagta ttgttggcgg tttattccaa tttgctgcat ggacaaagtt cgactatcct
    40981 gatttgtcgg ttattcaatg tttgaaatat ggctggtttt tattgaaaga tagaattggt
    41041 acgtatattt tgttgcagct gtcttttatc ggctggtatt tgctaggagc aatcgctttg
    41101 ttcatcggtc ttttgtgggt cattgtttat gtgaatgtgt cagttgcaga attttatgaa
    41161 caagcacgga ttgaaaaagg aggtccagac gcctatttta cttagcatgt aagcagttta
    41221 ttcatcaaaa aaataacttt tgtggtagga taaatctttg agggggaatg tacgatgaaa
    41281 ttaactgttt taggatgctt aggagcgtat ccttataaaa atcaagggac aacaggttat
    41341 ttgcttcaat ctgacgggtt caatttgttg atcgatgcag gtagtgctac gctgatcaaa
    41401 ttacaagaac accttgatcc acttgatcta gatgctgtga tccttagcca ttatcatcat
    41461 gatcatattg ctgaccttgg tgtattacag tattactggc aacttcatcc taagagaaat
    41521 ccggagacat tgctttcaat ttatggacac acaaaagatt ccattcactt tgaagaattg
    41581 acgttggaaa atgtctcaaa aggacatcct tattttgaag ctgaagaatt aaaaattggt
    41641 ccttttctga ttacttttat ggaaacaatc catccagtag tatgttacgc catgcgtatc
    41701 gtagaagaaa caacggggaa agtattcgta tttacaggtg attcaggtta tttggaaagt
    41761 ttcgctgatt ttgcgaaaga tgctgatttg tttctagcag atacctatct ttttgaagga
    41821 aatgaacatc atcatgccca tttcacttca aaagaagctg gagaacttgc gaaatcagct
    41881 aacgtaaaga aacttgtact tacacaccta cctcagtttg gtgatttaga acaattgaaa
    41941 aatgaagcgc aaaaggcagc aggcaaaggg attgatatag aacttgctgc agttgacaaa
    42001 gtgtttaaca tttaggagga agaacatgat ttttgtacca aatgaaaacc acgacccaag
    42061 aatcaattta gcaattgaac aatatttgct gcaagaaatg cctttagatg aacctatttt
    42121 gctcttttat atcaatgaac cttcaatcat catcggcaga aaccaaaata cgcttgaaga
    42181 aatcaatcga gattatgtgg aagaaaatgg cattcatgtt gtccgtcgct taagtggagg
    42241 cggagctgtc taccatgatt ttggaaacct gaactttagt tttatcatgc ctgatgatgg
    42301 ggattcattc cgtgactttg cgaaagtaac acagccaatc atccaagcac ttcatgaatt
    42361 gggtgttgct ggggcggaat tgaaaggccg aaatgatcta gtcattgatg gcatgaaatt
    42421 ttctggcaac gcgatgtatg ctaccaacgg tcggatgttt gctcatggaa caattatgtt
    42481 cgactctgat atcaatgaag tagtcaatgc attaaaagta aaaaaagaca aaatcgaatc
    42541 aaaaggaatc aaatcgatcc gttctcgtgt aacgaatatc aaaccatttc ttccagaaga
    42601 aaagcaaaaa atgacaacag aagaatttcg tcaagaaatc ttgttgaaaa ttttcggcgt
    42661 agagtcagta gaagaagtaa aaacgtacga attgactgat gaagattgga aaaaaatcaa
    42721 tcgaatctct gaggaatatt atcgaaattg ggattggaat tacgggaagt ctccagattt
    42781 caatttctct cgccaaaaac gtttttctat cggttcgatt gaagtccatt tgaatgtctc
    42841 agaaggaatg atcaaagaag caaaaatctt tggcgatttc ttcggactag gagatatctc
    42901 tgacgtggaa aaaaaattgg ttggacagaa atatgataaa gcatctctat caaaaatagt
    42961 cagccaaatt gatgtgaaga aatattttgg tgcgatagaa ccagaagaat tgcttacctt
    43021 actttattga aaataaaaaa tagcggaatc cacatatatg gattccgcta ttttttatac
    43081 aggattcggg taatttcgag agagttgaac agtgatccga tcaatgaaag tatctttttc
    43141 ctgcgttagt gttcctgtgt aacgatccgc tacgtctttc atcatcatta agtcaaaacg
    43201 ttcctgatca tctaattctt ctgtgatgcc tcgactaaat aggtttgtat cgtaatgtgc
    43261 actatgttcg caacaaatga cgaaatgttc ctcttgttcg tgtatcgaca aataaatttt
    43321 tcgatacagt ggattttcga tggcatgagt ttctctaaag gaatgctcta aagcgtggat
    43381 caaaagttga gccagatcgt catcgttgat aggaagttct tcgggaagat ctgcttggaa
    43441 ggttatctga atattccgtt tggcagctaa cttctggtaa cgcgctaaga taagatttgt
    43501 cacctggtgg ttgcaaaaag aacacagcat ttcgaaattt tttgcttctt ctaatatctt
    43561 ttccacatag tttttagctt ttgtttggtc cgtatgcatt aaatcttgca gattctccat
    43621 ctcttggaca aattccaggc gaagtgtatt tacttcttgg atatgctgtc gaagctgctc
    43681 ataatgttgc tccaacaaat catttttgat ttttaagaaa tctacttgtc tccgatcttt
    43741 gtcggtattt aagatgtgat cgattagatt gtagctgata ataacgatta gaatgataat
    43801 atataaaatc gtagaccaat tgatcgttgt ttgatagata cctaatgccg tttggatgta
    43861 aatcaagcaa tggataatgg ctaccaagac gatccaaggt gtacaagcta cataaaaacg
    43921 gttattctta tatgcttcgc caatgcatgc aagtgaagtg actaaagtgc ttataacact
    43981 agcaatatta gcaatcggca ttaattcagg aagttctgca ggagtaaaag ttgcataaag
    44041 cagaacgccg ataaaaatca aaagttggaa cgtacaaaaa attccgtacc attttttata
    44101 gtaaaacatt cggctccaat agtagcagat gatgaaagct gacgttaaga taataaataa
    44161 atgagataaa aaagaatgaa cgaatggttc aaaaagaata ccacttaaaa tatcttctga
    44221 tacagcttca aatcctaatg ccaaagcaaa gcaacttaag agaataagtg agtattcaag
    44281 acgtttttgt ttatacatca agtagatcgt atagaaaaag gtccctatag cgataaaaac
    44341 agcgattatc atcgtcaata cttgcggcac tgattgtgaa taaataaaac taatcaatgg
    44401 tccagttgtt cctagataaa ctttcggtgg cagtccagca tagttctgat aaggagatga
    44461 gactgtaagg gtcaattttt ttccgatata atttgctggt aaatctatca cagccaatga
    44521 gagtccggga ttgctttctt cctgataatc tttccgttgg tataaaatct tttcgcctag
    44581 atatacagta atccattgat ggtttccttt gattagtaaa tttgcttcgt ccaacggttt
    44641 ttcaagtgtc gtttccataa tgaatgtttc gtctttatcc acggtaggaa tcgtacgagt
    44701 gtacttgctg tcaaacttaa aatcaggatc atcttttgta tagaaaaccc acgaatcttt
    44761 tatttctttc atttgattgc tgtccgcagg agaaaaaata aaataaaaga aataagcaat
    44821 tcctgttaaa aataacaatg ataatataat gattatgctc cactttatac gtaaataaaa
    44881 agattctttt ttattatata tgttattaaa aaaagacata aaggtattcc tttctaaaac
    44941 ttgtaatgtt agggtaaacg ttttttaaac taacgtcaat tatttataat aaaaaatgag
    45001 ataaagatga ggaaatagaa ttagaaacat ataattatta aagggaaaca tttttacagc
    45061 tataatttga aaaaaattta tcgttattat atgattaaga aggattgaat aaagaaagaa
    45121 gttgaaaacg atacctgatt acaaaaaaat aacttttata agttctataa cgttgattat
    45181 atttttaatt cttattcttc tcgtcgtctt ggttccccga atttttggtt tgactcctta
    45241 tattattgag gacaaagaaa tgagtccata ttatgcacaa ggaaatctga tatatgtacg
    45301 accgaaaaat acaaatgaga ttttagtcgg agatattatt acatattatg aaaattcagg
    45361 aaaaaaaatc tcgacgcgtc gcgtaatagc cgttgaaaac aaccacgaag atttttatac
    45421 aaaagctgat acaaagactt atattgagcc tggagtagta aaaaagagaa atattatcgg
    45481 tagtcccatc tttcagattc catatattgg actagtcctt tcaaaaacgg catttgcatg
    45541 gattcagggg ttattcttta cgattgcttt ttgcctaaca ggaatcacag catggaatac
    45601 ctttgtagag ttgaaaaaga agcggagacg aacacagaaa tttttgtaag aatgttaaaa
    45661 acataaatgt attccagaga tccaacgact cttctgaaca tgaaacacga gaaaacactc
    45721 atgcagcttg tagatttagt cagttgttcg tataatgtgt gaagaggagg agtagtatgc
    45781 gcaatgaaat gatgcatcgt cctgtagtca aagaagaact attggatttt atgcgtaccc
    45841 agcaaaaaaa attaccgggt gagctaggca aactggaaga agaagctcat gtagcagaag
    45901 taccagttat tccccatgaa acagttgtat ttttgaaatt tttacttgga cagcttcagc
    45961 ctgaacgaat cttggagatc ggagcagcta tcggtttttc atcaagtgtc atggccacga
    46021 caatcggaga aaacgctcat gttacgacaa ttgatcgttt cgatgtcatg atcgagaaag
    46081 ctaaaaaaaa ctatgaacgt ctaggactga cagataaagt gactttgctt gaaggtcaag
    46141 cagcagatat cttgccggag ttgtctggtc cgtatgactt tatttttatg gatagtgcaa
    46201 aatcaaaata tattgaattt ttacctgaat gcttacgttt gttaagaaaa ggtggcgttt
    46261 tgatggtaga tgacatcttc caaggaggaa cgatcttgct cccagatgaa gagattcccc
    46321 gtggcaaacg agctatccat cgtaaattaa atgaatttct tcgtgttgtg atggatcatc
    46381 ccgatttgac atcgacaatc ttgccattag gtgatggtgt gatcttaatg acaaaggaat
    46441 cagaaacaat cgagttgtga tacatgatta tggcagcctg tagataatcg attgaatcga
    46501 gggctcaatt cgaacttgat gtaaaagttt ctatatttgt aagtcaatta aaggatacag
    46561 gaaatctaaa taaacaggag tagcatgaag tcaatgtagc tcctgttttc aattgataac
    46621 cttagaacag ctgaaggaga atgtctcgtt ttaaaagatt gatttagtag ttgggccaaa
    46681 ataattcatt atttgctaat taaataacta tttcgtattg acagaagcaa tttaattatc
    46741 tataattatt gatgttccgc tgattcttgt catagtagct cagctggata gagcatccgc
    46801 cttctaagcg gacggtcggg ggttcgaatc cctcctgtga cgctaaaaaa ccgttagaaa
    46861 ctgaggtttc taacggtttt tctttgtttt ttgtgccaat attgtttgtt ttcaaaggca
    46921 aataaaataa aaaaacatcc ttatcgagta caattataag gagcaaaaaa ataaaactca
    46981 tcagaaaaga ggaagaagat atgacgatac tatctagaat tttagtgact ctagttgcaa
    47041 ttgaattttt ttatatcatg tatatcgaaa cggtgagaac tgattctgac acgacaagta
    47101 gggtttttaa aatgagtaaa gaagagttaa gcagaaaatc tgttcaaaca ttgtttaaga
    47161 accaaggagt gtacaatgga ctattaggag tgggactact ttatggagct tatctttctt
    47221 ccgcatctaa agaaatcaca agtatgctat taatatctat tttttttgtt gcattatatg
    47281 gcagtttgac tagcgatcgt tggattttac tcaaacaagg tgggctagct ctgttggcat
    47341 tgctttctat tttcttataa atatagaaaa aataagaatt tatctaacag gaaactttgt
    47401 gaaaaatatt actttatctc atgaaatatg atattctaat gaatggtatt ttagaaaatt
    47461 tcatgaaaaa ggagttagat aggtgttcaa aaattatgtt tcctcactaa aaaaagaatt
    47521 ttcaggttat gatctttcaa gatttcagaa agatcttttg gctgggatca cagtagctgc
    47581 tgtagcattg cctttagcct tggcttttgg ggtatcaagc ggagctgatg cggccgcagg
    47641 tttgattacg gctatcattg ccggtatcgt tattggcggt ttatcaggcg gtttttatca
    47701 aatatcaggt ccgacaggtg cgatggcagc tatcttgatg tctattgcgg caacaaaggg
    47761 aatgcaaggt gtattgcttg ctactttttt agcgggtcta tttttacttg cagcaggact
    47821 tttccgatta ggaacactga cttcttttat cccagcaccc gtaattacgg ggtttacttc
    47881 aggtattgct atcattattg cattaggtca gatcgataat ttatttggtg ttcactcgga
    47941 aggtacaaac gttattgaaa aactaagtag ttatcaaacg ataggatttc cgataaacac
    48001 agcctctttg atcatgggag ggctagtcat tttagggatg tttatctatc caaaaaaatg
    48061 ggcgaaacga atgcctagtt cgctgctggc tatcattttg gcaacagcag ttatgctatt
    48121 gttccattta ccagtagcaa ctgtgggtaa gattccacaa acattgatca gtagtaatcg
    48181 tttgaatatg ggagatttta gcttttctgc cttgcaacaa gtggcagttc cagctattag
    48241 cattgcttta ttaggaatga ttgaaagctt gctatgtggt gcttcggctg gccgaatggc
    48301 aaataagccc ttagatagca atcaagaatt aatggcgcaa ggaattggaa atctgttgct
    48361 tcctttcttt ggcgggattc ctgctactgc ggccatcgcc cgtacaagcg tggccatcaa
    48421 atctggtgca caaactagat tagctggtat gatacatgca gttgtcttac tgctctccat
    48481 gcttgttttt gcgccagtga tgtcacagat tccaatgcca gcacttgcag gtgttttgat
    48541 tgtgacggct tggcgtatga atgaatggga aacgatcaag gagctattta ctaataaata
    48601 ttggtcagca gttctattgt ttatccttac gatgatttgt acagttatct ttgatttaag
    48661 tattgctatc gtgatcgggg tgatcggcgg ctgtgtattt tttgttgtca aaagcgctgc
    48721 tatcacgatt tcattagaaa cgatcgattg gcaaagaatg gggctgccag aaacggaaaa
    48781 gctgaataac tggacagtag tgtatattag tggtccactg ttttttatgt cttcagaaaa
    48841 actcaaaaat aaattggaag aactaaatca tcacgatggt gtcattttct ccatgcgagg
    48901 cgtaccaagt attgatttga cggcacgtgc agtttttgat gaatttcaag aaaaagcaga
    48961 gcaaagagga caaacgatta tctatacttc cttgcagcca gaagtagaaa aacaactttc
    49021 tcacttgtgg gaacatacat tcacgcatca acatcaaaca gttgctcatg cgttgacagc
    49081 tttacatgcg aaatatactg caattagcca ttaaaacaat gaaaaagaac agacaagact
    49141 gtaaactgaa gattttatgg tcttgtctgt tctttttaat attgctgtaa aagtgaagct
    49201 agtacttgcc agtcttgatg gataacaggt acaagggagc gattgtagaa aatagctgct
    49261 ggatgaaaaa gtggaagaat tcgatagttt ttttctgacc attcgtaggc atccttttgt
    49321 tctgttaatt gtaagacagg ccctgtatag agttgaccat gatttttggt aaccgtatat
    49381 tctttgccaa gtaaacgttg aagaccaatg tttccaagtg tcgcaattaa ttttggctgg
    49441 accttcatca gttcgtaatc caatattggt gcatgcgcaa atacttcgga acgagtaggt
    49501 gtccgatttg gataaacagt ttctgactgt tgtgtccgtt tattgatccg atgagtcacg
    49561 cgatacgggc ggctccttac agcactggtg atatacacct cttctcgtgt taagccagtt
    49621 gaagaaagtg cctgcatcaa ttcttttcca gcttgaccgc taaaaggaac atgcgtatca
    49681 atctctgttt tccctggggc ttctcccacg agcatcagtt taggatgaag aggtccttgc
    49741 ccagcgataa atccttctac aggacggccg ttcattctta aagaagcttc ttgtatcagt
    49801 tcttttgggt attccattta tgctcctcct ttttctttta ttataagaat aaaagaaaca
    49861 caatgcgact attttatttg gtagaactgt gaggatagaa aagcagaagt attgcttgaa
    49921 tttttgtttt actttttgat tttaaattta gttgactaac ggttgctgtt ctggtaagct
    49981 agtactgttg taattgtgga ctccacagct acaaccgctc agaacaagta ataagcacag
    50041 catatgctgt cacttcggat ggcgagtcta agtatatcag aaggaggtgc tttatagcat
    50101 gtacgcaatt attaaaactg gtggtaaaca agtaaaagtt gaagtgggac aagcaatcta
    50161 tgttgaaaaa cttgacgttg aagcaggaga aaaagttgtt tttgacgaag ttatcttagt
    50221 aggcggagaa tctacaaaag taggcgctcc aactgttgca ggtgcaactg ttgaaggtac
    50281 tgttgaaaaa cacggcaaac aaaagaaagt cgttactttc aaatacaaac ctaaaaaaca
    50341 ttcacaccgt aaacaaggtc atcgtcaacc atatacaaaa gtggttatcg acgcgatcaa
    50401 cgcttaatag cgtttgagaa agaaggccta tatatgatca aaggaacatt taagcgaaat
    50461 gacgcaggtc agatcgtttc attcactttg accgggcatg ctgatgctgg tccttatgga
    50521 agtgatatcg tttgtgcggg tgtttctgcg ttagcgatca gcactgtcaa cgggattgcc
    50581 tcattagccg gttttgagcc gattgttgaa atgaatgaag aagaaggcgg ttatctttat
    50641 accgaagtca cttcaggaat gacacaagaa caaaacaata ttgctgagat tctattagaa
    50701 aatcttttat taggattaca atcgatagaa gctgaaaact cagaatatat ccaaattaaa
    50761 acaattaatg ataaatagga ggtgcagacc atgttgttat ctatgaatct acaattattt
    50821 gcccacaaaa aaggtggcgg ttctacatct aacggccgtg actcagaatc aaaacgtcta
    50881 ggtgctaaac gtgctgatgg acaaacagtt acaggtggat caattcttta ccgtcaacgc
    50941 ggtacaaaaa tctatccagg tgtaaacgtg ggtatcggtg gagacgacac tttatttgca
    51001 aaagttgatg gcgtagtacg tttcgaacgt aaaggccgta acaaaaaaca agtgtctgtt
    51061 tatccagtag ctcaagaagc ttaattgaat cttatgatta aagaggttgt gacaagactt
    51121 ttgtcacagc cttttttgtt tataaaaata aaagttgtag tggaggctac gaagtttact
    51181 ttgtcttttt acacttaata tttttttgcc aatttggaat caggatgttt tgataactat
    51241 cgttttcaat tgatggaagc tatattgttt gctatcagtg aaataattat atggtaaaca
    51301 gtgttctttt tcttgtttcc attataaaaa tcgtatcggg atttttcgtt cttttaaatt
    51361 gaacatttct atctatccgc gtgttatcaa caaaacaaaa tcaggtaaat agttgacgaa
    51421 acagaaataa atacatagta ttatgttttg aagcctaagt acaatagtct atagaaaagg
    51481 agcgatacca ttggcaaaac gcattgaccg aatttatatg tatgtcaaag aaaaaaccgc
    51541 tcatttaaca aaggcagaat atgatcaagg agtaacaaca caggaaattg ctgaagtgtt
    51601 agggattcag cgaacaaata gcagtaaaga tctgaatcag ctagtgcgcg aaggaaaact
    51661 gataaagaca gacggacgac cagtacgtta catatatcaa acactcgtta cgcacgtaaa
    51721 gccagttaca aaacatgtag tcagttacaa agaaacgccg gtctctgaag aaaaaaatac
    51781 actccaaata gatactaagg atatctttgc aaaaatcatt ggagctaacg gcagcatgaa
    51841 aaattcagtg gaacaggcaa aagctgcgat tttgtatccg ccaagaggat taaattgttt
    51901 gattacagga cctaccggat caggaaagac ttattttgca cacgcgatgt atcattttgc
    51961 aaaagcgcat catgtaatag atgaggaaac ggaactgatt gttttcaatt gtgctgatta
    52021 tgccaataac cctgagcttt tgatgagtca tttatttggg tacgcaaaag gtgcttttac
    52081 aggcgcagat gaagaaaaaa tggggattat cgatcaagcg gatggcggta tgctgttttt
    52141 ggatgagatt caccggttgc cgcctgaagg tcaagaaatg atcttttact ttatggatca
    52201 tggaacttac agtcgtcttg gggaaacaac gaaatcccat gaagcaaatg tacggatcgt
    52261 aggtgcgacc acagaagacc caggttcttc tctattagaa acttttgttc ggcgtatccc
    52321 aatcaatatc aaacttccgg cttttgaaaa acgtcctgca aatgagaaag tcgatttagt
    52381 aaaaatcatg attgcccacg aagcaaaccg cacgcagcga aagatttctt tgacagaaga
    52441 tgtagcaaaa gcattgatcg gcagtgtgac ctatggaaat ataggacaat tgaagtcaaa
    52501 tatccagtta gtatgtgcac gaggatttct aaatcatatg caatcacccg aaatctcgat
    52561 cacaatcgat gatttgacag aagggattcg tagcggattg attcaattag caagcaaccg
    52621 cagtgcaatg gcagagcttt ctaaaatatt ggagccaaag ataactgtag caccaaatga
    52681 tacgatcatg aaaatccaat cggactccta tgagttacct tataatctat atgatatcat
    52741 cggagataaa gcagctttac tgaagtcgga tggtttggat caagaagcaa tcaatcattt
    52801 catttctaca gatatcaaca tccatctgaa gtctttctat aaagaccatg gtttttcttt
    52861 taatgcagac agcaaattag cagaatttgt agatccgaaa atcatagaag tgaccaatca
    52921 gatttatgta atggtcaaaa gatctttacc atatgagttt cagcaaaact ttatttatgc
    52981 tatgagcttg catatcagtt cttttttgaa aaaaatcaac ttaggagaag aacggcatac
    53041 gaatgataac atccgtgaga tggcttttga ttttccagat gaatatgctg ttgcgaaaga
    53101 agtgcgtagt tatatcgaaa cgtattttca agtggagatt ccagaaagtg aagactatta
    53161 cttgaccgtt ctactcgtct cgttacgggc aaatcaagca agcggcagaa tcggtgtagt
    53221 cgttgcagct cacggaaaca gcacagcaag cagtatggtc caagttgtcc aacagttgtt
    53281 agatgcagag aacgtacgag cagtagacat gcctttggat atggatccga aaacagcatt
    53341 ggaccgaatt gaacgcaatg tacaagaagt agatgaagga agcggggtca tactcttagt
    53401 agatatgggg tcccttgcgt cgtttaattc gcaaattcaa agagacactg gaattccagt
    53461 acgtactgtt gatatggtaa caacttctct agttttggaa actgtacgaa aggtctcagt
    53521 attagggaca gatctagata tgttgcatga atcattgaaa aattttcgag gttacgcaga
    53581 aatatcttct gtagaatctg ttcaggaagc aaaacctatt cataagaaaa aagctgtttt
    53641 ggctatctgc gcatctggcg aaggtacagc acaaagaatc aaagaattga tcgaacgagc
    53701 ggtaaacaag cgtcaagaaa cagaattaga agttctagct ttgtcgattg tggaagtgaa
    53761 agaagaactg ccagttatcc aaaaagaata tcagatcata gctactactg ggattactga
    53821 tttgaatatc caagccccat ttattccttt agaacgtttt attgatcaaa atatcgaagt
    53881 cattttggat cagttgctga tggaatcaga attggaggaa actgaattca tctccctaga
    53941 tgaagagtca gctaaaaata cgtgtgttga gtttatctct gataacttca tctttattaa
    54001 tggtagtaag ttaatcgatc caatgtggca attttctaca caaatctctc agacgacggg
    54061 aatcggcgat gaagaatatg gattcaaaat caacctagtg atgcacacag caggaatgat
    54121 cgaacgtatt attcggaatg aaccattgac tgtggaagaa aacgaattga caaatacaac
    54181 gaatgatcct ctttatagcc aattggcagc ctcagttgtt ctgttggaag atcagataaa
    54241 agtaaaagtg ccgatagaag aaatgtatta tctgctacgt ctagtccata atcagttaga
    54301 taaaaaagaa tacactgtgc catgaataca caaaaacgat acacttttat agtgtatcgt
    54361 ttttgtgtat tggttaagaa tcctttaaat tcaattagtg tatcgtaaaa aagttggcac
    54421 gctttttgct tatagtagtg tgtgctagga ggtggttgtt cacacagaaa tgataggggt
    54481 ttctgtgaag tagtgaggaa gaaggagttt tgcagatgga tattcgattg gcacgtatag
    54541 atgatcgttt aatccacggg caagtcgcaa cagtttggtc gaagcaaaca ggtgttgaac
    54601 gtatcattgt gatcagcgat gtagtcgctc aagataaatt gagaaagttt ctactaaaag
    54661 aagctgcacc gccggggatc acagcaaatg taataaccgt gcagaaaatg attcaaatct
    54721 atcatagtgg attattatca cgagcaaaag tgatgctttt attcaccaat ccccaagatg
    54781 ttgaagcggt tgtccgctgc ggtgtacagt tgaagtcgtt gaatatcggc ggtatgagtt
    54841 ttactgaagg caaatcaatg attaccaatt ttgtttcagt aaatcaacaa gatattgatt
    54901 cctttcgtta cttgaacagt caagaagtgg agttagagat tcgaaaagta cctgctgaca
    54961 ggaaaatcct tttgatggaa ctactagcga aaatcaaaaa ggtgtagcaa gaatggttgt
    55021 tggaaaaggt caaataattc tgttgtaatg gttagcaatt attaatattt ttgaatttgt
    55081 tacaacaaaa gtaaatagga ggtataaata tggtagggat tatcctagca agtcatggac
    55141 aatttgctga aggaatctta caatccggtt caatgatctt tggcgaacaa gaaaacgtca
    55201 aagccgtgat tttgaaacca agtgaaggtc cagatgactt gagagccaaa ttggaagaag
    55261 cggttgcttc ttttgataat caagacgaag tgttattctt agttgattta tggggcggaa
    55321 caccgttcaa tcaatcaaac acattattcg aagagcacaa agataaatgg gcaatcgtca
    55381 gcggtttgaa tttaccaatg ttgattgaag cctacgcatc acgcttttca atggaatctg
    55441 ctcatgaaat tgcagcacac atcattgaaa cagcaaaaga cggtgtaaaa gtaaaaccgg
    55501 aagaattaga accggcagaa gcaccgaaag cagcagttga agacgcacaa cctaaaggag
    55561 cacttccaga aggaactgtg gtcggagacg gaaaaatcaa atatgtatta gcacgtgttg
    55621 actcacgtct gttacatgga caagttgcga cagcttggac aaaagcagtg caaccaaacc
    55681 gtattatcgt tgtttcagat gcagtatcaa aggatgatct gcgtaagaga ctaatcgaac
    55741 aagctgctcc tccaggtgta aaagcaaatg ttattcctat tagcaaaatg atcgaagtag
    55801 caaaagaccc tcgttttgga aataccaaag cattgctttt atttgaaaat cctgaagacg
    55861 tattgacagc tgttgaaggt ggcgtagaca tcaaagaatt aaatgttggg tcaatggcgc
    55921 attcgatcgg taaagtcgtt gtaagtaaag tattgtctat gggacaagaa gacgttgaag
    55981 cgtttgagaa gcttgaacaa aaaggtgtga aattcgatgt acgtaaagta cctaatgatt
    56041 cacgcgataa catggatgat attttgaaaa aagcaaaagc tgaattagca aaagcgtaat
    56101 aaataatatt aaaatttata ggaggcttat catgtctatt atttcaataa ttttagtcgt
    56161 attagttgcc tttctagctg gtatggaagg tattcttgat gaatttcaat ttcatcaacc
    56221 actagtggca tgtacattga tcggtttagt taccggtaac ctcgaagctg gtatcgtact
    56281 tggcggtacg cttcagatga tcgctttagg ttgggcaaat atcggtgccg ctgtagcacc
    56341 agatgctgca ttagcatcag tagcatcagc aattatttta gttttaggtg gacaaggtgt
    56401 taaaggtgtt ccttctgcga tcgcgattgc cgttcctttg gctgttgcag ggttgttctt
    56461 aacaatgatc gtacgtacaa tcgctgttcc aatcgttcat ttgatggatg cggctgctga
    56521 agaaggaaac atccgtaagg ttgaaatgtg gcacatcatt gctgtatgtt tacaaggggt
    56581 tcgtatcgcg gtcccagcag cagcgttgct atttatccca gcacaaactg tgcaatcatt
    56641 ccttgaatca atgccagcat ggttaacaga tggtatggct atcggtgggg gaatggtcgt
    56701 agctgtaggt tacgctttgg taatcaacat gatggctaca aaagaagtat ggccattctt
    56761 cgttattggt ttcgttgtag ctgcaatttc tcaattaaca ttgattgctc taggtgcttt
    56821 aggtgttgca ttagctctta tttacttgaa cctttctaaa atgggtggtt catcaaataa
    56881 tggaggcgga agcaactctg gtgacccttt aggcgacatt ttgaatgact attaatcttg
    56941 aaggaggaaa ataaaatggc agaagaaaaa atcaaattat caaaaaaaga tcgtttagct
    57001 gttgcgtggc gctctacctt cattcaaggt tcatggaact acgaacgtat gcaaaacggt
    57061 ggctgggcat tttctatgat tccagcaatc aaaaaattat acaaaacaaa agaagaacgt
    57121 tctgcagcat taaaacgtca cttggaattc tttaatacac acccatacat tgcttctcca
    57181 attttagggg ttacattggc tctggaagaa gaacgtgcga atggcgcgcc tgttgatgat
    57241 gtagcgattc aaggggttaa agtaggtatg atgggtcctt tagctggtgt tggtgaccca
    57301 gtgttctggt ttacagtacg tccaatgcta ggtgcactag gtgcttcatt agctatggga
    57361 ggaaatatcc ttggaccaat catcttcttc ttaggttgga acattattcg ttgggcattc
    57421 atgtggtata cacaagaatt tggctacaaa gctggttcta aaattactga tgacctttct
    57481 ggcggattgt tacaagacgt aacaaaaggt gcttcaattt tagggatgtt cgtattagct
    57541 gcccttgtag aaagatgggt atcaatcaaa ttcttaccag ttgtatcaac tgtaaaatta
    57601 gataaaggtg cttacatcga atgggataaa ttgccttcag gtggtgaagg aatcaagagt
    57661 gcatttgaac aagttaataa tggcttagcc ctttctcctg aaaaggtaac aacattacaa
    57721 gacaacttag accaattgat tcctggttta gctgcattag gattaacatt gttatgtatg
    57781 tggttgctta agaaaaaggt ttctccaatc gttatcatcc ttggtttatt cgtagtcggt
    57841 gttgttggac acgtcatcgg acttttataa tccataaaaa tatagctcgg gcgttgtccc
    57901 gagctttttt tatatagtat actgttcata aacaaatatc tggagggaga aaatggttca
    57961 atcaatcaat acaaaagtag acttggtaat tgatgctact tcgtttacgg gtctgacaga
    58021 ttacgggcaa atcatgatag gtgataatgg ttttgagttt taccatgcgc gagatagacg
    58081 caaatatatc caaattccat gggaagaagt ggattatgta ctagcatcag tcatgctaaa
    58141 gggaaaatgg attcctcgat atgccattca aacgaaaaaa aatggtacat acacattttc
    58201 ttccaaagaa cctaaaaaag tgcttcgtgc gatcagagaa tatgtagatg aagatagaat
    58261 ggtacaatca ttgagtttct ttgatgtagt gaaacgtggt attcgtagta tgtttaaacg
    58321 caagtaaaaa ataaagaaaa cttctaagag gaggttttct ttattttttt actttgcaga
    58381 agaaaatatg aaatagcaaa accaattatt tgtccccttt aaatcccata tgctaaaatt
    58441 gattagcaaa gaaggaggtt atattatgag ttggttatgg acattgattg ttggagccgt
    58501 gatcggagca attgcaggtg cgatcaccag taaaggaaat tcgatgggct gtgtaacgaa
    58561 tatcattgct ggactagtcg gttcaagtat tggacaagcg attttcggcg aatggggacc
    58621 aaaagctgca ggtatggcac ttattccatc tattttaggc gcagtgattt tggtagcagt
    58681 tgtctcattt ttctttggaa aacgatcata agtttttaca aaatagtaaa ctaaaaggag
    58741 ttgcagcgag actgtgactc ctttttggct ttatttattt aaaataaaaa gaagttttcc
    58801 ggaaattttt atttaactgt cgtgtacgaa gaaaagtttg ctataatgga aaaaagcgtg
    58861 caaagaaaga gggagtttca ttatgatgct acgtgtagaa aaattgcgca aaaaaatgca
    58921 agaagaaaat ttggattcat ttttggttac cagtccgtac aatcttcgtt acttaacgaa
    58981 ctttacaggt acgacaggat tagctgttat tactttggaa aaagcatttt ttattactga
    59041 ttttcgttat accgagcagg cggcagcgca ggctcaaggg tttgaaatta ttaaaaatgt
    59101 cggacctatt tttgatgagg tagcaaactt agtacgcaaa gaagaactgc actcattagc
    59161 ttttgaggaa acaacagtct catttttaga atattcggtt ttagaagaaa tcatcgatgc
    59221 ggaattagta cctgtttcac agatgatcga agaactcaga gaagtgaaag atgaagaaga
    59281 aatcgctatt atcgaaaaag catgtcatat tgcggatatg gcatatgatc acatcctgaa
    59341 aatgatccaa ccgggcatga ctgagattga agtcgcaaat caactagact tttatatgcg
    59401 ttctcttggc gcttcaggtg tttcttttga tacgatcgtt gctagcggat tgcgttcggc
    59461 tatgccgcat ggtgtagcaa gcaaaaaaat catcgaacaa ggtgacttga tcacattgga
    59521 ctttggttgc tattacgaag ggtacgtttc cgatatgaca cgtacattcg ctattggtga
    59581 tcctggggaa aaattaaaag aaatttatca aatagtctta gatgctcaat tagcagttat
    59641 tgatgctgca aaaccaggaa tgactggtgt ccaattagat gcagttgcta gagattatat
    59701 ttcaaaacat ggttacgggg aagcatttgg acattctaca ggacatggga tcggtttaga
    59761 gatccacgaa ggaccaaacg tttcttctcg tgcagaaaag caatttgtac caggaaatgt
    59821 gatcactgat gaaccaggga tctacttacc aggaatcggt ggagtacgta ttgaggatga
    59881 cttgttgatc acagaagatg gaaatcgtgt gttgactcgt tcaccaaaag aattgatcat
    59941 cttatagttt catcaataga agaagtaagc gtaaaaatca gtgttcacag actttgttat
    60001 agaaaatcac tcgatttaat gatacactat agaagaactt ttaagctgta agagataata
    60061 aagttgggtg ttttctccaa ttcatagcag tttgcagctg atcggcggca gttttcttac
    60121 cacgtgatca ctatgccgca tcatagagaa ttcggatgta tctcgtgctt aggcaaaggg
    60181 aggaataaat aaatggcaga tgaaaaaaat ttgattttaa acgcaaacca agatttagga
    60241 gagatcgtga tcgctcctga agtcatcgaa gtaattatcg ggattgcagc ttctaaagta
    60301 gaaggcgtat acggaatgcg cggaactttt gccagcaatg tcactgaatt attaggacgt
    60361 gcagctcacg gaaaaggtgt ttacttagca aacgaggaag atggcttgaa agtagatctt
    60421 tactgttatc tagaatatgg tgtttcagtt cctaaagtag caatggaaat gcaggaacgg
    60481 gtcagccagc aagtcctttt catgacggat atcgatttat ctgaagtcaa tatccatgta
    60541 gtagctgttg ttcctgaaaa attagaacaa ccaacaatcg aagaactttt tgaagacgaa
    60601 gaggaagaga atgaataaag aattatcaag acacgagatt cgggaaatgg cattgcaggc
    60661 tttatttcca ttagacttca atgcagacct aacaaaagaa gatgcgattt tcaacgcaat
    60721 cgaattagac caccgcgaca tgatcaacga agatgaatca gagtttgttc ctgtttatct
    60781 tgatacgcta gttggcggtg tctgtgcaaa aaaagatgaa ttggataaag tgattgaaaa
    60841 acatttaaaa aacaattggc acatcaatcg gatctcaaaa atggatttgg tgatcttgcg
    60901 tatcgcgatt tttgaaatga cttatgtgac agatgtacca gcggctgttg ctttaaatga
    60961 agcaatcgag ttggcaaaaa catttagtga tgaccgttca cgtaaattcg tcaacggtgt
    61021 tttatccaat gtattgaaag aattagaagc tggggcgtaa gtcctagctt ttttctatga
    61081 atcatcataa acctgttata aatcgggaaa attctatggt agaatgaaaa cacaaaaaat
    61141 atctgggagt ggttagatgg cagaattgat taacggaaaa gaattagctg aaaaaatgca
    61201 ggcagagatt gctgaaaaaa tccaaaaatt gaaagataat ggtatccatc ccggattagt
    61261 cgttttattg gtaggggaaa atccagccag ccaaatctat gtaagaaata aagaacgtgt
    61321 agcaaaagaa atcggaatcc attcattggt agagcgttat cctgagacga tttctgaaga
    61381 cgaactactt gcagaaattg aaaaatacaa tcaagatcct agttttcatg gtatattggt
    61441 ccaattacca ttgccaaagc atatagatga agaaaagatc ctgttagcta tccgccctga
    61501 aaaagacgta gacggttttc atccgatgaa tttaggcaga ctccttgcgg gtaaaccgga
    61561 caagattcct tgtactccat acggcatcat gaaaatgttt gaagcttatt ctatcgatcc
    61621 ggcgggtaaa cgagcagtag ttatcggacg aagcaatatc gtcggtaaac cgatggctca
    61681 gctactcttg atggcagatg caactgttac tgttgctcat tctaaaaccg aaaatctggc
    61741 tgaactggca caagaagcag atattttagt cgtagcaata ggacgtggac attttgtcac
    61801 aaaagaattc gtaaaaccag gtgctgtagt gatagatgta gggatgaatc gtgatgctaa
    61861 cggtaagctg atcggtgatg tgaaatttga tgaagtggag cctattgcag gctggatcac
    61921 tcctgtgcca aagggtgtag ggccaatgac tatcacgatg ctgatgtatc aaaccgtaaa
    61981 atctgccgaa gcttttggag caggtgaata gatggcacaa gaatatttaa ctgtcactac
    62041 attaacgaag tatttgaaaa gaaaatttga tgccgatcct tatttagaac gcgtatattt
    62101 aacaggcgaa atatcgaatt tccgtttacg tgccaatgcc catcaatatt tcagcttaaa
    62161 agatgaccat gcaaaaatat ctgctattat gttcaaagga gcattccaaa aattacgatt
    62221 ccagccaaaa gaaggaatga aagtccttgt cgttgggcgt atttcattat atgaagcggg
    62281 aggttcttat caaatctatg tagagcacat ggagcctgat ggaataggtg ctttatatca
    62341 agaattggaa gaacgaaaag aaaagctgat gaaggaaggc ctttttcaag ggcctaaaaa
    62401 aatgctgcca cgtttcccaa agcgaatcgc ggtccttacc agtccaagcg gagcggttat
    62461 ccgcgatatc atcacgacgg tgaaaagaag atatccaatt gtccaattgg ttttgttccc
    62521 aacagttgta caaggaaacc aagcagcgga caatgttgtc aggaatatcc agagagtaga
    62581 agagatgggc aatttcgata caatgatcat cggtcgtggg ggtggttcga ttgaggattt
    62641 atggccgttt aatgaagagc gggttgctcg ggcgatttat caagctaata cgccggtgat
    62701 ttcatctgta ggacatgaaa cagataccac gatcgctgat ttagtagcag atgttcgggc
    62761 tgccactcca acagcagctg cagaattagc tgttcctgtt ttgtcagaag agttgatgcg
    62821 cattcaagaa cggcaagcta gattgcagca ggcgtttgta cgccagatcc agcgcaaaca
    62881 agaacggttc gagcgtgctc gacgttccta tgtatttcgg cagcctgaaa gattgtatga
    62941 aggtcagacc atcaaattag accagttgaa acagcgatta tatcaagcta cacagcaaat
    63001 ttatcatgcg aaagaaaaac aaacgatcgc attaagccac caattggaac aagtggctcc
    63061 gctttatcga gttaaagcag ctcgccaaga aactgcttat ctagaaaaac gcttagaaga
    63121 gaaaatccaa caatatatga aacaacagca gcagcgtttt gctcaagctg ttcaatctct
    63181 tgatttgttg agtccattga aaatcatggg aagaggctac agctatacga cgttaaatga
    63241 tcaagtagta aaaagtgtca ccgagattca tcaaggggat ttgctgacga tccattatca
    63301 agatggaatc gttaaaggac aagtacaaga gatagacaaa ggagaataaa aatatggaaa
    63361 atccaacttt tgaagagtca ctgcaagaat tagaaaaaat tgtcatgcag ttagaacaag
    63421 gagacgttcc attagaatca gctctagatt cgtttaaacg aggaatggaa ttaagcaaac
    63481 attgccaaga tacattagct aaagcagaag agaccttgac gaaaatgatg acagaatcaa
    63541 atgaagaagt tgattttgac ggaaacgagg aaagctgaga tgagattaac tgacttttca
    63601 gctattcatc tacctgctgt agaaaaagaa atcttgtctt ttttagatga gcatacaacg
    63661 gatagaggac tctatgatgc gatgagttat tcagtgaaag caggcgggaa aagaatacgt
    63721 cccttattac ttttaacggc cgttgcttcc tttgatgagc ctattgatgt tcccgtctat
    63781 caagtagcag ctgctttaga aatggtgcat acgtattcct tgatccacga tgatttgcct
    63841 gcgatggaca atgatgatct tcgtagagga aaacctacca atcataaagt attcggcgaa
    63901 gcattagcta tattggccgg agatggtttg cttacaggag cattccagct aatcagtatg
    63961 gcacacttag gtaattctcc taagcttctg ctgcttcagc aattagctgt ttgtgcggga
    64021 tcacaaggta tggttgctgg gcaagctgca gatatcgagg gcgaatcgaa aaagctttct
    64081 ttagaagaat tagcatttat tcatgaaaga aagacagggc atttgattcg ttatgccttg
    64141 ctggctggtg gcatattagc gaagcaacct gaagaaatct tattgctact tcaacgatta
    64201 gccgaacatt taggtttagc ttttcagata agagatgatc tactcgatgt gattggtaca
    64261 acgaaaacat tagggaaaac agctggtaaa gatgaacgta tggaaaaaaa tacttacccg
    64321 cgtttattag gcctagaaaa gacacgtgag gcgttggaga ttgagctgtt atccgccaac
    64381 aagatcatag acaaattaga agaggacgtt ttttcttttg atggcgggct gatgcgccag
    64441 atgatcaagc aatttgatgt ggagaaatga gggaaagaat ggaaaaagaa cgcgtagatg
    64501 tattagcggt caaacaagga ttatttgaaa caagagaaca agccaaacgt tcagtgatgg
    64561 ctggtttgat ttacaacgaa aaaaacgagc ggtttgataa accgggagaa aaaattccag
    64621 tgagtagtga attaagagtc aaaggtaaaa aacttcctta tgtttctcga gggggattaa
    64681 aattagaaaa agcactcaaa gcatttgatc tatctgttga aggaaaaaca ttgttagata
    64741 ttggtgcatc cactggcgga tttactgatg cagctttgca aaatggcgca aaaatgagtt
    64801 atgctttaga cgttggctac aatcagctag cttggaaaat aagacaagat cctagagtcg
    64861 tcgtaatgga aagagtgaat tttcgttatg caaaacccga agactttacc ttaggaattc
    64921 cagaagttgc tgttatcgat gtttctttta tttcactaaa actgatgctg cctccattac
    64981 atgctatttt aaaagaagag ggagaagtga ttgctttaat caaaccccaa ttcgaagcag
    65041 gcagagaagc tgtagggaaa aacggaattg ttcgtgaccc tcagacccat caaaaagttt
    65101 tagaagacat tttgtcattt gcggcacaag gtggatatga tgtgctggaa ttaagttatt
    65161 ctccgatcac ggggggagaa ggaaatatcg agttcttggc tcacttgagg aaagtacccg
    65221 aaagcggaac aatcaattca gctattaata tggcagaagt ggtttcaaat gcacacgaac
    65281 agtttgacca taaataaatt gtaactgtga aaatcaagtg agaagtatgc ggattcttct
    65341 ttattatacc aaggaaataa gctatcattg attaagaaga atatgctaag gagcgaagac
    65401 gtaatgagaa aaaaagatcg gcatcgtttg attactcgat tgttgagtga gcaagatatc
    65461 cgaaagcagg aagaatttgt tgaaatcctg aaaagcaaag gaatatctgt tactcaagct
    65521 acgatttcca gagatataaa ggaactgaaa ctgatcaagg taccggcttc agatggcggc
    65581 taccgttata gcataccagc tgaaacaaac gaagatgtaa atactaaatt ggaaaaacta
    65641 ttaaaagacg ctttcgtggc agttgaccaa atggaaaaat ttgttatttt gaaaacattg
    65701 ccgggaaatg cttcggcagc agcaaatttg attgataaac gttacaagaa agaattattt
    65761 tcgattatca atgatgacga taatgttttg atgattacac gaactgaaga ccaggcagtt
    65821 ttattgaaga aagacttcct gcattacctt taagttgagg agcgaatgtg atgctacaag
    65881 aaatcagtat tactaacttt gcgattattc cggaattgcg gctttctttt cacgaaggaa
    65941 tgaccgcgct gaccggagag actggtgcgg gtaaatccat tattatcgat gctttaggtc
    66001 tgttagcagg aggaagaggt tcaagcgatt atatacgtca aggagcagaa aaatgtgtgc
    66061 ttgaagggtt gtttgaacta ccaaaacaag aaggattctc tgaactgatg gttgaattag
    66121 ggatcgaaac agatgaggac aatctgattg ttcgacggga tatgtcctta actggaaaaa
    66181 atgtttgtcg agtgaatggt catattatca cattagctaa tctgagaaga atcggcagtt
    66241 atttagtaga tatccaagga caaaacgaac atcaggaact gctccagcct gaatcacatc
    66301 ttgctttatt ggatcgtttt ggagatgtag ctttccaaca gaagaaaaag agttatcagc
    66361 aagaatatat ctcttatcgt gagctggaaa aaagggtacg aaaagtgcag caaaatgaaa
    66421 aatcctatgt tcagagaata gatatgctcc atttccagca agaagaaata gctgctgctg
    66481 atttgcaagt cggagaagaa gagaaattaa aagaagaaag agaaaaactt agcaattatc
    66541 aaaaaattgc tgatggatta gctgcaggtt acggagcact gacgaataat gaacaaaaca
    66601 gtgtagatgg tgttggcttg gccgtttcag aactgcaagg gatcgcccat ctagatgtgg
    66661 agtatgaagc aatttatgaa aatatccaaa gtgcttacta tttgctgcaa gatgcaatcg
    66721 gcgatatgag tcgtcagatt gatttattgg agttagatga gagtcgttta gaagaagtta
    66781 cgcaacgttt agaattaatc cgacagttga aacgtaaata cggcgaatcc attgagtcaa
    66841 tattggctta ctatgacgaa atcaccgaag agctagcttc ttctgatttt tcagaaggtc
    66901 aattggacaa aatgaaagag gaactagagc aaaaagagct tctacttcag caacaagccg
    66961 cagatcttca tgaagcgcgt aaaaaaatcg caaaagaatt ggaaaaatcc attctgcatg
    67021 aattgaaaag tctgtatatg gaaaacactg agtttgaggt acgtttctta aaagaagaaa
    67081 atagacaatt gaatcatgat ggatttgacc aaatcgaatt ttacattacg accaatccag
    67141 gagagccact gaagcctttg gtgaaagtcg cttctggtgg cgaactgtct cggatgttgc
    67201 ttgctttgaa aactattttt tcttctgagc aaggtgtgac aagtattatt tttgatgaag
    67261 tggatacagg tgtgagcgga agagttgcac aggccatcgc agataaaatt tctcaaatat
    67321 cgaaatactc tcaagttttg tgcatcactc atctgcctca agttgctgca gtcgccgact
    67381 atcaatatta tattgtgaaa gcagtgattg atggacgtac gcaaacttct gtttcagagt
    67441 tgaaaacaaa agaaagagaa gaagaaattg cacgtatgct ggctggaagt gagattacaa
    67501 aactcacact cgaacatgca aaagaactat tgaaactcgc aaaaaggacc tgagccacct
    67561 cgcagtggtt caggtccttt tcagcagctg atagctatat tttattttta catgacactc
    67621 attacagcag tcaatacaac agatccaatc gttaaaatca gcattgtcca aatgacaaat
    67681 tttgttactt tagcaaaagt acttgtttgt tttttttcac gcattgtttt caacctcgtc
    67741 attaattagt tgattcaagt gtacaatctt ttttccattt ccacaagagg gcttttttat
    67801 aaaaacttcc ctttttttaa ataataggta gttacgatac aaataaataa tgttaataga
    67861 actagccata taaaggcatc atccctttca gcaataggta acttaatgtt cattccatag
    67921 atactgccga cgattgtcgg tatagttaaa ataatcgtta aagaagttaa aattttcata
    67981 atattattca gattattcga tacgattgct gaaaaagtct gattcatttg ttctactagt
    68041 tttaattgaa tgcgagaagt agtcattgcc tgctctgttt ccaccaatac atcacgtaaa
    68101 tgatttttca aagcatgatt ttcttggaaa agtttatttt gcgctagttc tttcaatgca
    68161 tctaaattag cagaagtcgc ttctctgaaa taaacgaggc ttttttggat atccatgatt
    68221 tgataaagtt gtttgttttc agtggatact tgaagtttgg cttccaaagt atcgcattga
    68281 gattgaagcg tagctaaata actgttgtaa gagagtgtga cctgccaaat gaattgaaag
    68341 aagatattga gattaggcga aatatcactt tttggaaagg tctggctaaa gactttcttt
    68401 aaaaacagtg gttcatgatt cgtaattgtg ataagcttct ttttgggcgt caaaataata
    68461 gagagcgggt atgtatccac ttgaaaataa ccgctaggac ttgttttagc aaaaggatat
    68521 tgtaacaata ataacgctgg ctttgtcaac tttgattgat tgaattcttc tgtacgggaa
    68581 ttttctgcat cgtccaatat ggaagtgatg taatctgacg gaatttcaaa ttgatgtgtc
    68641 agctgatgaa tctcttcttg attaggacgt tctacacaat accagatcgt ttggtcagtt
    68701 tcctttccgg tattacgcag tttctcatta tctaatgtaa aatatttgat catgattttc
    68761 ctcctgttgc ctgtttcttt tattataacg gatagggaat tggaatacat ggaatgtgtt
    68821 tgtaagatat agaatataac tgcataaatt taaataaaat gaaacagttg tgtaaagtat
    68881 atacagtata cttgaagcaa cgataaaaaa ggagtttacg ttcatgggca cattcacacc
    68941 acttatcttg ttttggtata tttacccggt aatcgttttg tttggttgcc aatttcttgt
    69001 aaaattattt tcgttaaatc gtcgttttaa aataaaagcc cctgatttgg cgattccttt
    69061 tttgtggggc ggtatgcatg cattgtctag aaatacaggg acaatttcta tcttaccgtt
    69121 tttctttatt tctgttttgc taatgggaat ttgtattgct gttttccaag catattatta
    69181 tgaagaaatt atttatccgc gttatttcaa gatgacttgg cgtttggttt ttctgttgac
    69241 aatcgtcttg tacgctgtct tgatcattgt gaacatactt tcttacctat gaaaaaaata
    69301 gaaaagctgt aaaaagtctt ttaaaaggct ttttgcagct tgatttttaa aaaaatgttt
    69361 atgaataaaa aaagttgttc ccggatttcc cagaaaaggt ggtagaaagt gggggattgt
    69421 ggtagactaa ttttagttag tggaggaatg gggggctttc aatgttgatg ggtgaattcc
    69481 aacataatat tgatgcaaaa ggacgtttaa tcgtgccctc aaaacttcgt gaagaacttg
    69541 gcgaaaagtt tgttctgaca cgcggattgg atggctgtct tttcggctat ccgatgagcg
    69601 aatgggaaaa tctggaagct aaactgaacg aaatgccgtt ggctaaaaaa gacgcacgta
    69661 cttttgtgcg attcttctat tcggctgcca ctgaatgcga acttgataaa caaggacgaa
    69721 tcaatatacc cagcacgtta agaaattatg cagctttgac aaaaggttgc gtagtcatcg
    69781 gtgtatctaa ccgaattgaa atttgggatg agacacgatg gcaagatttt tctgcagcag
    69841 cagaagagaa ctttgacgaa attgctgaaa gtatgattga tttcggacta taagaatagg
    69901 aagaggctga ctaatgactg aaacatttca acactataca gtcatgttga aagaaacggt
    69961 agatggattg aacatcaaac cggatggcgt ttatgtagac tgtacactcg gcggtgccgg
    70021 acatagtgaa tatttattaa gccaactaag ttctaaaggg catctgtatg cgtttgacca
    70081 agatcaaaaa gcaatcgatc acgccaagct gcgtttagct tcttatgtcg aacaaggtat
    70141 ggtcacattt atcaaagcga acttcagaga attagaagaa cgcttgagtg agcatgtaga
    70201 taaggtagac ggcattttgt atgatctagg ggtttcctct cctcagctag atgaagcaga
    70261 acgtggattt agttatcatc aagatgcacc gttggatatg cgcatggatc agtcagctcc
    70321 tttatccgct tatcacgtaa ttaatgaata ttcctaccat gaattagtaa agattttctt
    70381 ccgctatggc gaggagaaat tttccaaaca aattgctcgt gaaatcgaac gagtacgaaa
    70441 aacaaaacca atccaaacaa ctggtgagct tgtcgaaatc attaaacaag tcatcccagc
    70501 tcccgcaaga agaaaaggcg ggcatcctgc aaaaagaatt tttcaagctg tgcggattgc
    70561 agtaaatgac gaactcggtg ccgaagaggc ctctttagaa caggcaatcc ggttattgaa
    70621 agtcaatgga agaatcagcg taatcacatt ccattcattg gaagacagaa ttgtcaaaag
    70681 tatgtttaaa gaatatagta cggtacaaga tctaccaccg ggacttcctg tcatacctga
    70741 cgaattccaa ccagaactga aagtcattac tcgtaaacca atccttcctt cagaggaaga
    70801 gctaacagaa aacaatcgat cacgcagtgc aaagttacgt attgcagaaa aaataagaat
    70861 gaaagaggag tagaatgatg gcagaattaa agaaaatgca agattacgaa tatgatttac
    70921 ctgaattggt ggatcagccg gaacaacaaa tgcctgattc ttcttcgaat caagaaatca
    70981 ttcttccaca atcaccggca aaacgattaa agcagatttc tgctttggaa aaaattgctg
    71041 tggcaagcgt ggcgattgct attgttgctt tatgtgtttt aacagttatg ctgcgcacaa
    71101 atatcagcgg agctgaaaaa gaaatcacgt caatacaaac agaaatcaat gataaaaagc
    71161 aagaaaaaac gagtttagaa caagaaaaaa acgaactttc tcgtactgaa cggattaaac
    71221 aaatcgcaga agaaaaagga ttgtctatta atgacgacaa tttaaggaaa gtgaagtaaa
    71281 tgagtcttaa aaataaaatt cggcggttta tggaaaagaa aaatctaaat ccgatgaaca
    71341 atcgcaaaaa agtaggtatc attttatttg ctacgagtat tggattgttc tttttatttg
    71401 ccgtccgttt ctcgtatatc gttattggag gtcatgttgc tggtacctca ttagctgaaa
    71461 aaacaaaaca gttgtaccaa ggaagtgaag tagtaaaagc aaaaagagga acgatttatg
    71521 atcgtaacgg tgtggcgctc gcagaagatg cctcctctta ttcaattaaa gcgatactct
    71581 caaaaacata tacctctggg gataaaaaac tgtatgttga agaaaagaat tttgataaaa
    71641 tagcagaaat cctgcataag aatctttcta tagacaaaaa agatgcactg aatattttag
    71701 aggacggcgc aaaaaaagag ctgtatcaag ttgaatttgg tagctatggg aaaaatatct
    71761 cccaagaaac caagcaaaac atcgaagcgg acatgaaaaa agaaggtgtc gctggtcttt
    71821 attttgtcga ccatcaggct cggatgtatc caaatggcgt cttttcttct cattttattg
    71881 gatacgcagt tccagacaaa gatgagaatg gcttagttgg taaactaggt ttggagtctg
    71941 cttacaatga tatcttgagc ggtaaagatg gtaagattat ttatcaaaaa gataattttc
    72001 aaaatcctct gccaggtaca gtggcagaag aagaaaaagc tgtcgacgga caagatatct
    72061 acacgacttt agacagcaga ctacaaagtt atttagaaac actgatggat caagtaaatg
    72121 aagaatatca gccagaagaa ttgacagcag tcctgatgaa agcaaaaacc ggagaaatct
    72181 tggcaatggg acaacgacct actttcaatc ctgaaacaat ggaaggattg actggaaaag
    72241 atgctatctg gcgtaatttc ttggtacaag atagttatga accaggatca accatgaaag
    72301 ttttcactac tgcagctgct atcgaagaag gagaattcaa cgagaatgaa accttccaat
    72361 ctggaaaaat ccaagtagaa gatgctacga tcaatgacca tgactttggt gaaaaaggtg
    72421 tgctgaccat gcgtcaagct ctttcatggt caagtaacgt cgggatggtc atactggaac
    72481 agcgattagg cggacgatgg tataactatt tacaaaaact gggatttgga caaagtactc
    72541 attctggatt agatgatgaa gtaaacggtg ctttacctac attaaatatt gtcgaccgag
    72601 caatgagtgc ttatggacaa gcggtaggtg tgacgaattt ccaaatgatg aaagctttca
    72661 cttctattgc gaataacggt acaatgattc aacctcgata tatcagcaaa gtggtggatc
    72721 cccaaactgg agaagagcga acaactcaaa cagaagttct agggcaacct ttttcaaaag
    72781 aaacaacaga aaaagtccgt gaatatatga gagatgttgt cgaaagcgaa aactacggta
    72841 gtgcttatgg tgtatatagt gtacctggat acaatgtatc ggcaaaaaac ggaacagccc
    72901 aaatcgcttc tgatacaggg ggatatcaaa caggagatac agcctacctg tattcgattg
    72961 tggagatggt tccttctgaa gatccagact atgtcttgta tctaacaatg aaacacccta
    73021 agacatatga tcgaatggca ttggctaaaa tcgcaaatcc attgatgaaa cgggcaatgg
    73081 attttaaaga aactgaagaa gattcagata cagaaacaaa aacagaaaaa gtatccgtag
    73141 ccgattatcg taatcttgaa gcggacgttg cagcagctga tgctcaaaaa agtggactgc
    73201 aacctgtagt gatcggtaat ggtaaaaaag tacaaaaaca atctactgcc aacggtgatc
    73261 agttgatctc tggagaaaaa ctgatcctat atactggtgg tgataagctg atgcctgacg
    73321 taacaggatg gtcaaaagca gatatcatga agctggggaa aatcctcggt attgaagtta
    73381 gttttgatgg agatggttat tgtgtcaaac aagaacttgc tccatatgaa aaaatcactg
    73441 aagacaaact tagttttact ttagaagaat aagaagagaa ataggagaga aaagagcatg
    73501 gaatggacac aagcattgat tcccattgtc agcagttgtg caatgacaat tgccgcaatg
    73561 ccgctattta ttggttattt tcaaatgaaa aagcaaggtc aggcgattcg tgaggaggga
    73621 ccaaagtggc acaatagcaa agccggaact cctacaatgg gcggcttagt gtttctgatc
    73681 ggctctattt tgacaggaat ttgggttgga gcttggcaaa aacagctaac tcctactctt
    73741 ttcatcttgc tttttgtatt agcgttatac ggagtaatcg gctttcttga tgattttata
    73801 aaaatattca agaaaagaaa catgggactg aactcgaagc agaaattgat tggacagatt
    73861 ctagggggaa tcatctttta tcttgtttac cgttctgaag gttatccagg tgtattgaat
    73921 ttcttcggac tagatctacc tttaggaatc atttatggta ttttcgctct cttctggctt
    73981 gttggatttt caaatgcagt aaatctgaca gatggcatcg atggattagt tgctggctta
    74041 ggaagtatct cttttgcagc ttacggtcta attgcttggc atcagcatca gtatgatgta
    74101 ttaatcattt gcttaagtgt tttaggaggt ttactgggtt tcttcgtcta caacagaaaa
    74161 cctgccaaga tttttatggg agatgtcggt tcattagcgt tgggcggact tttagcggca
    74221 atttctataa tgctaaacca agaatggaca ttgcttttga ttggtttgat ctatgttatg
    74281 gaaacagcaa gtgtgatgct tcaagttact tcctttaagc ttacaggaaa acgaattttt
    74341 aaaatgtctc caattcatca tcactttgaa atgtgcggat ggtcggagtg gaagattgat
    74401 acagtttttt ggttgatcag cattgtgact tcgttgatca cattatggat cgtttggtag
    74461 gagatgacgg gaaatgaaaa aaatatcaac atatgaaaat aagaaagtgc ttgtcttagg
    74521 actcgcaaaa agcggagtaa gtgcggccaa attattacat gaattgggcg cacttgtcac
    74581 cgttaatgat ggaaaacctt ttgacgaaaa tccggaagca caagagctgc tttctttagg
    74641 gataaaagta ataacaggta gtcatccaat cgaattgttg gatgaagaat tttcattgat
    74701 ggtcaaaaat ccagggattc cctacagtca tcctttagta gcaaaagccc aagaaatggg
    74761 tattccggta attacggaag ttgagctagc ttacgaagta gcggaatgcc caattattgg
    74821 gattacagga acgaacggaa aaacaacgac aacaacgatg acaggactac tactgaatgc
    74881 aggagcagat caaggaattg cacgtttagc gggtaatatt gggtatccag caagtggtgt
    74941 agcgcaagaa gcaaaatcag aagataaaat tgtgatggaa ttgtctagct tccaactcat
    75001 gggaatcaca gatttccgcc ctcatatagc ggtgattacc aatatctatg aagcacatat
    75061 tgattatcat ggtacaagaa aagaatatgt caaagctaaa tggaaccttc aaaaaaacat
    75121 gacagaaaaa gattatctga tccttaactg gaatcagtca gaactacaag aattagcgca
    75181 aagaacaaaa gcacgcgtac tgcctttctc gacaaaagaa gtgttggaag acggtgtgta
    75241 tgctgacgat tactcaatat actataaaaa agaaaaaata atggaaatat ctgaacttgg
    75301 cgtaccaggt aagcataatg tagaaaatgc actagctgct atttctgtag caaaattgta
    75361 tggtatatca aatgaagcta tacgagaaac gttgcatttt ttccatggcg taccacatcg
    75421 tacgcaatat gtaggagaaa tccaaggcag aaaattttat aatgattcaa aagcaaccaa
    75481 tattttagcg actaaaatgg ctctaagtgg atttgaaacg tctaaggtcg tgttattggc
    75541 tggcggatta gacagaggaa atacatttga tgaattaatt ccttcattaa aaggaatcaa
    75601 agcaatggtc gtatttggcc aaacaaaaga aaagttgatg gatgctggaa aaaaagcagg
    75661 catcgagaca atagtaacag ccgattctgt agaacaagca gttcctttag cattagaaaa
    75721 cagtacagat ggtgatgtag ttttattatc accagcgaat gccagctggg atcaataccc
    75781 aaattttgaa actagaggaa acagattcat ggaagctgtg aaccgattaa aatagaaaaa
    75841 gtagaagcga gcgataaaaa atgaaaattt tggttacagg cggaggaaca gggggacata
    75901 tttatccagc tcttgccttt gtcaattatg taaagactca agaaccgaat gctgaattca
    75961 tgtatgtggg agcaaaacga ggtctagaaa acaaaatcgt ccctgataca ggaatgcctt
    76021 tccatacctt ggagattcaa ggatttaaac gaaaaatttc catgcataac atcaagacgg
    76081 tccgattgtt tcttaagagt atccgtcaag caaaaaaaat cctcagtgat tttcaaccag
    76141 atatcgttat cggtactgga ggatatgttt ccggagcggt agtgtacgct gcagccaaaa
    76201 tgggtattcc aacagttatc catgaacaaa acagtgtacc aggtgttacc aataaatttt
    76261 taagccgata tgtggatcgg atcgctcttt catttaaaga tgcggcttcc tttttttctg
    76321 aaaacaaagc agttcttgta ggtaatcctc gagcacaaga agtagcagat actaaaaaat
    76381 cagatatttt gaaaacattt gatttggatc cagaaaagaa aacagtcttg atttttggcg
    76441 gtagtcaagg agcacttaaa atcaatcaag cagtatcagt ttttttaagt gaatatattc
    76501 cttcttctta ccaagttttg tatgcttcag gagatagata ttatgaagag atcctaaata
    76561 aaataaatcc tgaaatacaa aatattagtg tccagcctta tatcaaaaat atggcagagg
    76621 tcatggcttc cagtgatctg ctggtggggc gtgctggtgc aacttcgatc gcagaattca
    76681 cagctctggg gttaccggca gtgttggttc ccagcccata tgttacgaat gatcatcaga
    76741 ctaaaaatgc aatgagtctt gttcacgcag gagcagcaaa aatgattgca gataatgaac
    76801 taacaggtga atcactaagt cagacagtca acgaaatcat gggtgatgaa gaattgcaaa
    76861 agcaaatgtg ccgagcttct aaagaacaag gaatccctga tgcttcaaaa cgtctttatg
    76921 acttagtaaa acaaataatc aaaaaataaa ggcggtggtc ttagtgagta agaaaaaaga
    76981 accaaaaaaa ttgtctcatc cgtcttctga tgaaaacaat ttgactcctt ggcaaaaggc
    77041 taaccgaaaa tatctggcag aacaagagat gaaaaaccaa gagccaaaaa aagaagacat
    77101 aacattagat acaccccttt cagattctga agaagaaaaa acggttcaag aggaacagca
    77161 agaagaaagc cagtccgaga aaaaaatcga attttttgaa gagatcaagc aaaagaaaaa
    77221 cggtggacca tataacggtt cttttttgaa ccggctgccc aatttaaaaa gtcaacggca
    77281 caaagtacta tttcgtcggt tagctttgat catcactgta ttgggtatac cgctgatttt
    77341 tttgatctat tatgtttctc cttatagcaa attacaagca gttgcagtat caggcaataa
    77401 gacagtcaac agccaagaaa tcatctctga tacaaaattg tcattaggag aaaatgtttg
    77461 gggacagtat tttcatcgat ctacttatat cgatcgcctt aaaaaagcac agccaagaat
    77521 cgaaacagca aatatacatt tcaaagggat gaatgaattc gatttggatg taacagaata
    77581 taaagagatt gctttgatcg caaaaaacaa tcaatactac ccagtgattg aaaatggaac
    77641 agtattagat gaaaaagtag caaatcctac aaaaaatctc cctatcttgg aagagttcaa
    77701 agactctgca aaaatcaaag aattaacaaa gcaatacaat cagttatcat cagaattaca
    77761 aaaagcaatt tctgaaatca agtacacgcc aagagcaagc aataaaaatc tgatccaatt
    77821 gaatatgaat gacggcaatc aagttattgt aaatatcaat aatcttgcga accaaatgaa
    77881 atattattcg caagtagcca aagatatgga tgaaaaaggt gtgatcgaca tggaagttgg
    77941 tatcttctcg catccttacg gtagcactca agaaacggaa gaatcgggcc aatcggaaga
    78001 aacaactgaa agtgaagaaa atgcggatca aacaaacgaa tcaagtgaag aggcagaaac
    78061 gcaaaaagca caaacacctg gatcgtctag cacaaacgga tcatctgatt caaatgattc
    78121 aagtgaaaca agtcaaatag aagataatcc aagtacaact ccgtcaagtt taaatgaaaa
    78181 ttgataaaac aactttaaaa agtaagaatg tactttctaa aaaccagtaa tttatggtag
    78241 aattggaagc agtgaatata tctattaata agtatatttt aaaacggacg ctagttgttc
    78301 gcttgtatat ggaatagtaa gtaggaggga atcccattca tggcaaaaac aggaatgtat
    78361 gtaggccttg atattggaac gacatctgtt aaagttgtcg tggctgaata tatcgacagc
    78421 caaatgaata ttattggagt aggaaacgca aaatcagaag ggattaaccg aggcattatc
    78481 gttgatatcg ataaaacggt ccaagcgata caacgagcag tacgacaagc agaagaaaaa
    78541 gcaggtatcc agatcaaagg cgtaagtgtc ggtttgccag caaacatgct tgaagtagaa
    78601 aattgccagg gaatgattgc agttaacgga gattcaaagg aaatcaccga tgaagatgtc
    78661 agaaatgtgg cttcagctgc ccttgttcgt tcaatacctc ctgaacgtca aatcgtgtca
    78721 atattgccac aagattttac tgtcgatggg ttcgagggaa tcaaagatcc tcgtggaatg
    78781 atcggcgtaa gattagaaat gtacggtctg ttgtttacag gacctaaaac gatcatccat
    78841 aacatacgta aatgcgtgga aaatgccgga ttaatcgtca acgaaatggt gattactcct
    78901 ttagcgctga ctgaatcaat tctttctgac ggagaaaaag attttggtac gatcgtcatt
    78961 gatatgggtg gcggccaaac aacaacggct gttatgcatg ataagcaatt gaagtttacc
    79021 aatcttgacc aagaaggtgg cgaatttgtc actaaagata tctctattgt attaaatacg
    79081 tcatttaaca atgcagaagc gttgaaaata aactacgggg atgcataccc agaacggaca
    79141 tcaccagatg aagaatttcc agtagatgtg attggtcagt cagaaccagt gaaagtcgat
    79201 gaacgctact tgtcagaaat tatttcagct cgaatggaac agattttcaa caaagcaaaa
    79261 gatgcattgg accaaatcga agcattagag ttgccaggtg gcgtcgtatt gacaggcgga
    79321 gctgctagtc ttcctggtgt tgtggacttg gcacaagaaa tcttcggcgt aaatgtaaaa
    79381 ttgtatgttc caaatcacat ggggctgaga aacccggtat ttacaaatgt aataagtatc
    79441 gtggattatt cagcaaactt gagtgaagtg tatcaattgg caaaaattgc tgttacgggt
    79501 gaaacctcag cagctcgtca aatggtagtt gaacaagaaa caacaaacac atatgagagc
    79561 tatgaagcac ctgaagaaac aatctacgat gagccggaac caaaagagtc cggagaaaat
    79621 gtaaaaaata aaatcaaggg tttctttaca aatatttttg attaattcat cctgggagga
    79681 ataagatatg gaattctcaa ttgataataa tatcaatgac ggcgcagtaa tcaaagtcat
    79741 cggagtcggt ggtggcggtg gaaatgctgt aaaccgaatg attgaagaaa acgttaaagg
    79801 tgttgaattc attacagcca acacagacgt gcaagcatta aaaaattcaa aagctgaaac
    79861 agttatccaa ttaggcccca aatatactcg tggtttaggt gctggttctc agccagaagt
    79921 tggacaaaaa gcggcagaag aaagtgaaca atccttacgc gaagctttag acggtgcaga
    79981 tatgattttc atcactgcag gaatgggtgg aggaaccggt acaggtgctg caccaatcgt
    80041 tgcaggtatc gcgaaagaat tgggcgcatt gactgttggt gtcgtgacac gtccattcac
    80101 ttttgaagga ccgaaacgtg gccgttttgc tgcagaaggt atcgctcgtt tgaaagaaaa
    80161 tgtggataca ttattaatta tctcaaataa ccgtttatta gaagttgtag ataaaaagac
    80221 accgatgctt gaagctttcc gtgaagcaga taatgtttta cgacaaggtg tacaaggtat
    80281 ctctgatttg atcacagcgc caggttacgt taaccttgac tttgctgacg tgaaaacagt
    80341 gatggaaaac caaggtacag cattgatggg gatcggtgtg gctagtggag aagatcgcgt
    80401 agtagaagca acgaaaaaag caatctcctc tccattgctt gaaacatcta ttgatggagc
    80461 tgaacaagta cttcttaaca tcacaggcgg tttggatatg acattgttcg aagcacaaga
    80521 tgcttcagat atcgtagcaa atgcagcaac tggcgatgta aatatcattc taggtacatc
    80581 tatcaatgaa gaaatgggcg atgaaatccg tgtgacggtc atcgcaacag gtatcgatga
    80641 atcaaaaaag gaacgcaagt ctagccgacc agctagacaa gcacaaatgc aatcacctgc
    80701 tcaaaagact gtattagata tggatcaagc taaaccaact tcttcagaag aagaaaacag
    80761 ttttggcgat tgggatatca gacgtgaaca aaatgttcgt cctagagttg atgattccaa
    80821 ttttgacaat atcgaaaaaa aagaatttga tacattcaac cgcgaagaaa caaaatctca
    80881 tggcgatgat gaattaaata cgccaccgtt tttccgtcgt aaaaaataaa ggagaggaag
    80941 agcacatgat tgctgacaac ttgcagaaaa tcaagcaaga aattcaggat tcgtgtgctc
    81001 ttgttcatcg tgatccgaaa gaagttacat tagtagctgt aacaaaatct gttgaatccg
    81061 ctcaagctga agaattggtt agagctggcg ttatgaattg tgcagaaaac cgagtagata
    81121 aattattaga aaagaaacaa gatttatttg attttcctga aataaagtgg catttaattg
    81181 gtaatttaca gcgcagaaag gtaaaattga ttgtaaatga aattgattac tttcatgcgt
    81241 tggacagttt aagattagca gaagaaatcc agaagcggtt agagaaagaa ttatcctgtt
    81301 ttattgaagt aaatgtttct ggtgaggaat caaaacatgg aatttcacca gatgagcttt
    81361 taccgtttgt ggaatcttta gctgcctgca caaaaatcaa ggttgttggg ttgatgacta
    81421 tggcgcctaa agaagcttct gatgagcaaa tccataaagt gtttggtacg ctgaaagaat
    81481 tgcgtgatct tgttcaagca aaaggttatg cccatgcacc atgtagtgaa ttaagtatgg
    81541 ggatgagtca agactttcaa attgcgattg aagaaggtgc aacatttatc cgtgtaggaa
    81601 cagcgctatt caaagacgaa taaaggagga acaaaaatgc cgcttttaaa cagaggagca
    81661 attgctaatt ttttcggatt agctgatgag gaagagtacg aataccaaga ttatcctgaa
    81721 acaacaaaaa aggctgtaca gccaactaaa aaaaatacaa cacagaatca atcaactcat
    81781 tctgtatctg ctgcgaatac tcatgaaaaa caaaaaacgg cgacttcacg tcctgtaacg
    81841 catgaaagta ttcagccggc tgaaaagaaa aaagtaattg aactacatca atcacctaca
    81901 aaaactgtag cgaaagaaag aacagcttca agaacctcta cacaacctaa agcaaatctt
    81961 gctggaaaaa tcactgtaat ggaaccaaga gcttattctg aagctatggc gattgctaaa
    82021 cgaatcattg ctggagaagc aaccttagtg aattttcatt tagtagaaga aaaacaagcg
    82081 agaagaatcg ttgattttct aacaggaaca gtttatgcac tagatggaga tatcaaacgt
    82141 gttggagatg agatttttct ttgtacaccg actgggatgg agattgacag ttcaaccgct
    82201 caatcttttg cagatgcgaa tttgtttgat ctgtaagagg aggaactacg atttatacta
    82261 ttgcactatt acttcattta cttcaacggg cagcctattt ctatagcatt atcttgatgg
    82321 tatatgcatt gctctcttgg tttccaggcg ggtatcaatc agggctagga cgattattag
    82381 ctaagatctg cgaaccgtat atcagtttgt tcgatcgtct gcctttgagg attggaccct
    82441 tggatttcac aattttaggg gcaatcctga tcttgaactt agccgtacaa ggtttagcac
    82501 agatcgttat atggataact gtccatttag tttattgatt gaagcaacaa aatctttatt
    82561 ctcagaatag ataggagggg aaaattttga tgcaaatgta taccagcact ttcgggcaga
    82621 tgaacgtaca tttatcgatt cggtaggcga ttggattgaa caggttcaag gacaatatgc
    82681 accctatctg accgaatttc ttgacccaag acaatcctat attttagaaa cactgattcg
    82741 acaaagcagt gatttgcgtt tcatgtttta tggcggatat gaacaggcag aaagaaaaag
    82801 atgtttgatt ttccccgatt attacgaacc tgtagaggaa gattttgaaa cagcattgta
    82861 tgaaatccat tatcctagca aatttgccag cctaagtcat gggaaaatcc ttggaacttt
    82921 agtgggaacg gggatcaaac gctcttactt tggcgatatc atctcggacg gtgatcgctg
    82981 gcaagtattt attgcaaatg aggtggatca ttttgttgct gcacaagtaa caaagattgg
    83041 aaaggttgct gtacgcttgg aaccaatcaa atacacagaa atgattatgc cgaaagatag
    83101 ctggacacaa gaaagaggaa cagttagttc catgcgacta gacagtgtga tctctgagat
    83161 ttataatatt tccagacagc ggtccaagca gctgatcgaa gctggtaaag taaaagtgaa
    83221 ctggacagaa aatacaagac ctgattttgt acttgaacta ttagatatta tttcagtcag
    83281 aggttatggc agagtccaga tccaagaact tgaggggaag acgaaaaaag aaaagtacag
    83341 ggtactgttc ggtgtattac gtaaataaaa taaaaaggtg gtttactcat ggctttaact
    83401 ccattagata tccaaaataa gacattcccg acaaaaatgc ggggatataa tcaagacgaa
    83461 gtagatgatt tcttagattt agttgttcgt gactatgagg aattgactca aagaaatcga
    83521 gaattagaaa aagcagtcaa acattctgaa gaaaaacttg agtacttcaa tgaattaaaa
    83581 gatgctttga accaatcaat catcgtagct caagatacgg ctgataaagt aaaaacaagt
    83641 gcaagcaagg aatccgaagt gatcgttact tctgcacaaa acaaagcaga tgagttagtt
    83701 gcgaacgctg aaaaacgtgc acatcaatta acaactgatg cagaagaaaa agcaagaaaa
    83761 atcttgacag atgcaacaga aaaagcaaga cagcttgcga cagaaactga agatcttaag
    83821 aaaaaaacac gagtattcca tcaacgtatc agcttgatgc ttgagtctca attagaacaa
    83881 gtgaaaagtc cggaatggga tgaaatttta caaccattct ccagctacgt gactgacagc
    83941 catgaagtga tcaaagaagt tttagcaaaa cagcttgaca atgaaaatga tactgatgta
    84001 aactcagaca caggagtcat agaagcacct atgacggcat ttgatacaca ggctattgat
    84061 atctcgatcg tgcctggtga aaatgaaaac gagtagaaca gaaaagcact ttgttattct
    84121 taaagcgagc cgatgagagt ggaagttcgg taagacactg catggtgaaa gatcctctac
    84181 ttgagacctg cttttccgaa attagagtaa gaagcagcgg ctgacctcgt tattggtcac
    84241 agagagtttg cactagatag atgcaactaa atttgggtgg taccacggca tgttcgtccc
    84301 ttgcggctga acatgctttt ttgtgtccgg aaaacaaata aaggagtgta tggttgaatg
    84361 aaaatgaaag aaaccttgca attaggaaaa acaaaattcc ctatgcgtgg taatttgcca
    84421 aaccgagaag gcgagtggca aaaagaatgg gaagaaaatc gaatctacca aaaaagacaa
    84481 gagttgaacg aaggaaaacc tagctttgtt cttcatgatg gacctccata tgccaatggt
    84541 aatatccatt taggacattc attgaataaa atcagtaaag acattatcat tcgttcaaaa
    84601 tcaatgtctg gctatcgtgc accatatgtt ccaggatggg atacacatgg attgccgatt
    84661 gaacaagtat tggctaataa aggcgtgaaa cgtaaagaaa tgtgcatggc agaatacttg
    84721 gaaaaatgtc gtgaatatgc actttcccaa gtagataagc aacgagcaga tttcaaacgc
    84781 ctaggagttg caggagattg ggaacatcct tatgttacat tagatccttc ttatgaagct
    84841 gcacaagtac gcgtatttgg taaaatggct gaaaaaggct atatttacaa aggattgaaa
    84901 ccaatttact ggtctccgtc aagtgagtct tctttagcgg aagctgaaat tgaatataaa
    84961 gatgtaaaat ctccatctat ttttgtagct ttcaatgtca aagacggcaa agatattctt
    85021 gatgaagata cagcttttgt catttggacg acaacaccat ggacattgcc agcaaaccaa
    85081 ggtattgctg taaatccaaa atttacgtat gtattggtgg aagccgatgg ccgtaagttt
    85141 gtcgtagcga aagatttgct agaaacagtc caatcagaaa tcggttggga aaatgtaaca
    85201 gttctaaaag aatttgctgg acaagaaatg gaatatatga ctgcaagtca tccattctat
    85261 gaacgtgaat cacttgtgat tttaggcgat catgttactt tagacgctgg tactggatta
    85321 gtccatactg ctccaggaca tggggaagat gactatatcg ctggtagcaa atataaacta
    85381 tctgtagtgt cacctgtaga ctcaaaagga ttgtttacag aagaagcacc tggatttgaa
    85441 ggtatctttt atgacaaagc aaatcctatg atcactgatc tattaaaaga aaaaggagct
    85501 ttgctgaagc tagatttctt tactcatagt tatcctcacg attggcggac gaaaaagcct
    85561 gtgatctatc gggcaactcc acaatggttt gcatctatcg ataaattccg tcagaatatt
    85621 ttggatgaag tagaaaaagt agagtgggtt atcccatggg gtaaaacacg tttgtataat
    85681 atgatccgtg accgcggcga ttgggtaatt tcccgtcagc gtgcatgggg agtaccactt
    85741 ccgattttct atgctgaaaa tggagaagca atcatcacac cagaaacaat cgaacatgtg
    85801 gcaaaattat ttgctgaaca tggatcaatt atctggtttg aaaaagaagc gaaagaactt
    85861 cttcctgaag gatttaccca cccaggttct cctaatggcg aatttacaaa agaaaaagat
    85921 atcatggatg tctggtttga ttctggttct tctcatgaag ctgttttacg tcagcgacca
    85981 gaattatcat tcccagcaga tatgtattta gaaggatcag accaatatcg tggctggttc
    86041 aactcaagta tcacgactag tgtggctatc aatggtgtag caccatacaa atcagttatt
    86101 tctcaaggat ttgctttaga tggtgaaggc cgtaagatga gtaaatcttt aggaaacatc
    86161 attgcaccag aaaaagtgat caaacaattt ggtgcagata tccttcgctt atgggtatct
    86221 agtgtagatt atgaagcaga tgttcgtgtt tctatggata tcttaagtca agtggcagaa
    86281 gtatatcgta aaatcagaaa tacgatgcgt ttcttattag cgaatacaga agatttcgat
    86341 cctaaaaaag atactgtttc gtacaatgat ttacgttctg tagacaaata tatgaccgtt
    86401 cgattgaacc aagtaatcaa agaaatccga gaagaagggt acgacaaata taacttcatg
    86461 catatttacc gtacagttat gaacttcttg acggttgatc tatcgtcatt ctacctggat
    86521 tttgctaaag atgtagttta tattgaagca gaagatgatt atcaacgtcg ttgcatgcaa
    86581 actgttttct atcaaacagc tgtagctttg acaaaattat tgactcctat cattccgcat
    86641 acagcagaag aaatctggtc attcctgaaa gaagaagagg agtacgttca gttaagcgag
    86701 tttcctggtt atgaagagtt tgcaaaccaa gaagaattga tggatacctg gactgctttc
    86761 atggacttcc gtgataatgt cttaaaagca ttggaagaag caagaaacag caaattgatt
    86821 ggtaaatctt tggaagcaaa actaactgtt tatccaaaac aacaagttcg tgaaatgtta
    86881 aaagctttgg atgcagatat tgcacaatta ttgatcgttt caccagacta ctttgaagtt
    86941 atggcagcag atgaacaagt gccagataac gctatggtat ttgatgatgt tgctattaca
    87001 gtggaaaaag cagacgggga aacttgcgat cgctgtcgcc aagttcgtaa agatgtagga
    87061 gtagatgaaa aacttcctca tctatgcggt cgttgtgcaa aaatcgttga agacttctat
    87121 cctgaagctg tagctgaagg attcgaagaa aagtaaaagg gaaaaggggg tgtgacatgt
    87181 tctttgtcac accccctttt gttatcaatc gtttaatttt ccacgttcac gataccagtt
    87241 atctggttgc cagatccatt cgaatccgtc tttttcaagt agttcgaaag ctgctttagg
    87301 tcccattgat ccagctttgt agttgggaaa atccggttgt gtttggtccc aagcttttcg
    87361 aatgacatcc acgatatgcc aagattgagc gacttcttcc caatgagaga agttcgttcc
    87421 atcaccattc aacgcatcta atagtaattt ttcgtaagct tctgggctat tttcaaccat
    87481 ttctgcacta tttctaaatt ccaatttgat tggttccgtt tcaaatcctt gccctacttc
    87541 tttcccattc aaggataaag aaaatccttc agttggctga atatagatcg ttagaacatt
    87601 aggtggaagt gtcgtatcgt cacatggttc atcgacagat gttttgaata cattaacagg
    87661 aacttgtttg aatacgatgt tgatccgagt ccctttttca gtcaaacgtt tgcctgtacg
    87721 tacatagaaa ggaacacctg accaacggaa gttatcgata gtgaatttgc ctgcgacgaa
    87781 tgtttcagtc tctgattgag catccacttt gtcttcttcg cgatagccct tgaattgtac
    87841 accatccaat tcaccttgag catattgccc gcgaacgaag ttttctaaca cttcttcttc
    87901 gttataaatg cggatagctt tcaatgcttt gatcttttct gtacggattt ctttttcgga
    87961 aaatgctact ggcggttcca ttgcaagtaa tgctacaacc tgtaaaatat gattttgcac
    88021 catgtcttta agtgcgccac tatgatcgta gtagcccccg cgatcttcta caccgaggtt
    88081 ttcgccaaaa gtaatttgga tattgtcgat atagcgattg ttccattgag cctcaaaaat
    88141 attattggca aaacggatag ctgagatgtt ttggatcatt tcttttccta aataatgatc
    88201 aatcctgaaa atatcttgtt ctgggaatac ttctctgatt tgctggttca attcaaaagc
    88261 agactggtaa tcagaaccga acggtttttc tatgatcaaa cgattgaagc cattttttgt
    88321 cataatattt tgtgatttca aatgagaaac gatcgttcca aaaaattgag gtgccatagc
    88381 caaataatag acacggttac cgtttaattg atagtgtcca tctaatcggt cagacaattc
    88441 ttttaatgtc tgataatgcg ttgaatcatt tacatcatgg gattgatagt aaaaatggct
    88501 tgcaaaggta gttgcttctt catctgaagg attcaatgca cggattgttt ctttgacaac
    88561 ttcacggtag tgctcatcgc tccagggacg gcgagcggtt ccgattacag caaaatgctc
    88621 gcctaaattt ccttttctgt aaaggcggaa caaagacggg tacaatttac gctgggctag
    88681 gtcacctgta ccgccgaata tcgtaaacaa tacatttttt tcattagaca tgtggataca
    88741 ctcctttata ttttatcgac aaaaatcgtg ctggccgcct taaagctgac ggggattttc
    88801 tttctatcat tttggatagt gatcggtcct tcataagctg cgatgtcaac gatctcatat
    88861 tcttcgttga tattcaggtc aatagtgact aagtaatcca acaattcttt ttcatcaagc
    88921 actcgtgcga tacgcacttt tgtaccgaca ggataggact ccaatgtttg tcggcgttct
    88981 tcatggacaa cttcctcttt tttaggaatc gatcctccat gaggacaatg ttcaggatga
    89041 ttcaaatagt tttctaaatg atctgcaaga tgttccgaag tcacatgttc caatacttca
    89101 gcatcatcat ggacttcatt ccagctatag tctaaatgtt caactaaaaa aacttcccat
    89161 aagcgatgtt tgcgaatcag actgctagct ttttgcagtc cgcttgcagt caattgtaca
    89221 ccttgataag gggaatgttc tacaagctct tctttcacaa gtttagaaat catttcactg
    89281 acagatgcag ctgagacatc tagactagat acgatttgtt tattgttgac tttcgttgta
    89341 tctccaccta gttctaaaat gattttcaaa taatcttctc gatttggtgt catattcgat
    89401 catcctttca tggtaaaaaa ccatcattag taagtgtaac aaaaaaaacg ttaattgact
    89461 atttttctaa ataaaaaaca aaggattttt agacgttttt acaacaaaaa acgaaagatg
    89521 atagagaaga ctcattcttt cgtttataaa ggatatatat atacattggg agggatctat
    89581 ttttcagata acaacttata tgaagtgtct gcagattcgt cataaagagt ttgttcggga
    89641 taagaaaaga taagataaaa accatactga tcacgtacga ctaaacgata ttcatctatt
    89701 tctaccacag atgtgccaag agactgaccg ttgatcaaaa acgtgtaatc agatgtaatc
    89761 tgtaattgtt gtttttgctg gctatcacta aagaaccatg taccttgata ctttgagagt
    89821 ccttctgact tttccgcttg ttttttcttt ttcaagtaaa taaaacttga gagaacggta
    89881 atgagtgaac caagaaaata gatcattttt tttatcacca aatcactcct ttatgagaaa
    89941 ataatcgcat agacatattc tttcttcttt atttcattgt accgaataaa agctaaaatt
    90001 tctctaagac tttcgactgg tttttttact gtttttctac aaattatgat aggatacata
    90061 gtaggaagaa aggtgacata atttgaagta tcagcaagtg gaaatagctt tttttattga
    90121 ccgcccgaat cggtttatag cacattgtat gaaaaaagac ggagaaataa ttattgcgca
    90181 tgtcaaaaat acaggtagag gaaaagaggt cctgatacca ggcgctgaag tggctattgt
    90241 atttgctcct ggacctaaac ggaaaaccgc ctatgatctg attgctgtaa aaaagaaggg
    90301 tgactggttt aatatcgata gtcaattgcc taatcggctg gcaattgatg gtattttaga
    90361 cggcacgatc cttttaccag gattaactgg tccatttacg gtatacaagc gtgaagtaac
    90421 ttatcttcac tccaaatttg atatttattt agagacggtg gtcggacaaa aagcatttgt
    90481 tgaagtcaaa ggtatgacac ttgaaaataa agcgattggt gcttttcctg atgcgcccac
    90541 actcagagga ttgaaacata taggagaatt aacgtatgcc gcacaagatg gttatgccgc
    90601 ttatgtcttg tttattgctc agttcgaaca tcttcatctg gcaacgatcc atgaagaaat
    90661 gcaaccagca ctagctgata tggttcgaca tgcgcagcaa agcggagttc aaatcctcgc
    90721 ctataattgt caagtatcgg cagatgaggt aactgtcaag caggccattc catttgatgt
    90781 agatatccct tttgaagatc ctatacaata gaaagaaggt aaaataatga tcaacttagg
    90841 tattatcgga acaaattgga ttactcatca atttgtccaa gcagctttaa gcacaaaaaa
    90901 atatgaattg acagcggtat attctagaca tttggaaaaa gcacaggaat ttggttctca
    90961 ttatgaaggg gatatcgaat tcgcaacaga tttgaagact tttttcaata ttactcatat
    91021 ggacaccatt tatattgctt ctccgaatag tcttcacttt agtcaagcta agcaagcaat
    91081 tttggcagga aaaaatgtga tagttgaaaa gccagctttt tcgacgccaa aagaaatgga
    91141 tgaaatcatc catttggcaa atgaacacaa tgtgtatttt tttgaagcag caagaaatat
    91201 ccatgaatca ggttttaaaa agattgccga ttacttacca ttaaaagacg agataatcgg
    91261 tgcaaatttc agctatatga aatattcttc gcgctatgac caagtattag atggagaaga
    91321 accgaatatc ttctcgactc atttctcagg tggtgcattg atggatttag gtgtttacct
    91381 tgtatatgca gctgttgctt ggtttggaat gccaaaagaa gtccattatt tcccaagaaa
    91441 gatttctact ggtgtagatg gtcttggatg ggggatctta cgttatgaga attttgatgt
    91501 atcgatccag ccaggtaaaa tcggtgattc ttatttaccg tcagagattt attttgactc
    91561 aggaacgcta gtgatgaatg gagtcaatgc aattgaacaa gcacaatttc atgatcgcgc
    91621 gcataaagaa atcattgatt tggcggtaac agctgcagaa aatccaatgg cagatgaagc
    91681 agaggatttt gccaatgtca tggctcatcc gaaagatcca gcttggggat tgttgtatga
    91741 agagtggatc gagctagcta gaaatgtgaa ccaagtgatt tatgatttaa gaaaaaatgg
    91801 cggcatcaaa ttcgatgcag ataatgagga agatttttaa tgaacgaatt tgtacaagta
    91861 tttcccgaaa attggcagaa aaaatatgag gataaagggt ttatctcacc ttctcttatt
    91921 caacgagcag tttttcagcc actaagtaat aaacaaagta tagtagcggt ttctcctaca
    91981 ggttcaggta aaacactggc ttatctttgg cctctactac taaacgtgga accaggtgaa
    92041 gcaagtgcgt tggtcatttt tgcttcttct caagaactag ctattcaagt agcagatgtt
    92101 gcacgtgaat ggggaaaaga caaagagttg aaagtccaat ctttggtggg cggagccaac
    92161 gtcaaacgtc agattgaggg tttgaaggaa aaaccagaaa tcttgattgg tacaccagga
    92221 agaatattgg aattgatgaa agcaaaaaaa ataaaagcac atcaagtgaa aacaatggta
    92281 tttgatgaag cagaccagct atttgacgct gggaacagcc aaatcattga tcaaattctt
    92341 catcaagctc caacagagta tcagctagct tttttttcag cgacagcaga tcgttccctt
    92401 gaaagcattg aaaaaatcac tgggaaaacg tttgagacaa ttgacgtgac agcagaggac
    92461 gattctcgaa aaggactgag acattacttt ttacgtgtac cgatacgaaa gaaagatgac
    92521 tacttgagac gtctcacgca tatagaagat tttcatggtc tgattttctt taatcaactg
    92581 actgaattag gcgtaatgga agaaaaattg ctgtatagag gtgtaccggt aggaagtctg
    92641 gcctctgatc agaacaagct actccgaaaa gcagcgctag aacaattcaa aaagaaaatg
    92701 gtcagagcac ttttgaccac agatgtggcg gctagaggtt tagatattac aggtcttcct
    92761 tatgtagtga atgcagaagt tccgttaagt gaagaagctt atttgcatcg ttcgggaaga
    92821 actggtcgaa tgggtaatga aggaatagta attacactcg tacaagataa taatctaaga
    92881 gatttaaaac ggcttttacg tccatcaaat ataacacttg aagagatttt tttatacgga
    92941 ggtaagctgc aaactgagca gccaaccaaa gaagatgcag ctcctgcagt aaataaaaag
    93001 tatgcgaaaa ctttttctgg aaaggagcag aacccagaat ataacaaaga aaaaataaga
    93061 aaaaaagtta aaaataaatc aaaaaaagag agaaataaag gagcaaggcg caaataagcg
    93121 cctttttatg ctatgattat gaaaacggga acaaaattgg ttgccagagg aggggttatg
    93181 tgaaagaacg tcaagaagag atcttgcagc tattgacaga aaaccaacaa ggattgacag
    93241 caagtgatgt agctgagcgc ttaactatcg atcgaagcaa tgcaagccga tatttaagtg
    93301 aactatacaa ggcccatcat attgtaaaaa ccgctggccg accagtcgtt tactctttgc
    93361 caacggaaaa gtcaaaatct gatgaagtgc atgtcgactc ttctacgcaa gtgacttttg
    93421 aaacactcgt tggagaaaat gattctttaa aagtcagcat acagcaggca aaagctgcga
    93481 ttctttatcc accgcggggg ttgcatacga ttatttttgg agaaactggt acagggaagt
    93541 cgatgtttgc tgaatgtatg tatcattttg caatcgattc agagatgctt tctgcagatg
    93601 cgccatttgt ttctttcaat tgtgcggatt atgctcaaaa tccgcaactg ctctttggac
    93661 acatctttgg gatcaaaaaa ggggcttaca caggagcagc tcaagatagt cccggtctaa
    93721 tagccaaagc agacggcggt atattgtttt tagatgagat ccaccgttta ccgccggaag
    93781 ggcaggaaat gctgtttact ttcattgata aaggtattta ccgtccgctt ggagaaagtg
    93841 cacaagtaca tgaagcatct gttcagatca ttggggcgac gactgaatct tcagaaagtt
    93901 tcttatctac gttcaatcga cggattccta tggcaatcac cttgccgaat cttgcttcac
    93961 gttcactgga tgagcgttat gaaatcatct ctctttttat caaacaagaa gcgaatcgtt
    94021 tgaatcaacg tatcgatatc gaaaaagaag cgattcttgc cttcatgctt tatgatgcgg
    94081 aaggaaatat cggtcaagtg aaacgagact tgaaactagt ctgtgcaaaa tcttttttac
    94141 attaccgtac tcatcatgag aaaaagctaa tcattcgaaa agaagaactt tcccttcaag
    94201 tacaaaaagg attactgaaa atcaaggaaa taccggaacg tctcgaccga ttcatggaca
    94261 gcaaaagtca gtatttgaca tttgaaccag gatttgctga tgttgtctgg tcacaagatc
    94321 cagaacgaaa catgcaggtc tataacgata ttgaggaaaa agtcacttct ctttccgaaa
    94381 ccggtgtgga gaacattgat ctagagaccc tcatttcaaa agatgtggat gcttatttcc
    94441 agacatatgt caaagaattg actaaaagtt cgattcccaa agatcttttg ccagatgaaa
    94501 tctggcggtt aaccaatcaa ttgtatgatg tggctgaaaa acggttagac cgtatttata
    94561 atgaaaaagc tcgttttgct tttgctttgc atctgcaaag tacattggat cgtgtgagag
    94621 aaggtcatat gatcgtccac ccagatttga ataacgtacg gaaaaatttt aagagggaat
    94681 tccaggtagc aatcgatctt tcaacgatca ttgaagaaga agaacaagtt gagattcctt
    94741 ttgatgaaat cggctttatt agtatgtttc tttctatcaa tctaggagaa agtgagcctg
    94801 ttaaagaaaa caaagtagat gttctcatat tgatgcacgg cagacagact gcttcaagca
    94861 tgttggagat ggcacaagaa ctattaggaa caaaagtagg gaaagctttt aatatgccac
    94921 tggttatgga agttcaaaca atgtatgaag aagtgttaca atatgtcaaa aactatcaag
    94981 agcatttgac gaatggccta ctcttgttaa cagatatggg atcattaaac accttcagtg
    95041 atttgattta tgaagagact ggcattcgga cgaaagctat cacgatgaca agtaccatga
    95101 ttgtgataga agcgatacgg atggcaagtg ttggaagaag cttagaagat atttatcaaa
    95161 atatccagct ttcctttgaa tcgatcgtac gtgatcagtt caaagcggaa aagtctgaaa
    95221 ctttacagcg taaaaaagcc gtaatcgtga catgctttac tggtgaagga gtagctgcca
    95281 aattatatca gcgtattatg ccagtgatta atcaaaataa ggtagaactg atccagatgc
    95341 agtttttaga acgtgaatca ttcaaaaaac acatcgatgc cttattggaa gaatatgaga
    95401 tcaaagcaat tgcgggaaca gttgatgttg attatcaaaa tatcccattt ttttcagcct
    95461 atgacgtttt tgatgatgaa aaactaaata ttttaaagcg tatagctgat gatgagatcc
    95521 cgacagctaa aattgtcgat tcacttgctg gatcattaag cgatgtcttt tctattgatg
    95581 aactgatccg ttcattgcaa aagacagttc agcagatcca gaccgatttg tcgattatcg
    95641 tggaacctgg agtagatact ggaatcgtta tccatttagc cttcatgtta gacggattga
    95701 tcaaacaaga aagccatcgg caatttgaga aactgtctat tttctcaaaa atttatcgtt
    95761 tggaaatgga tatcgttcgg gcaaacttga tggcactaga acgaaaatat caggtaaaaa
    95821 ttccagaaga tgatatcgca tttcttacac aaatgtttct tgaaaataaa ataaaaaaat
    95881 gaacacacta atcatactgt ttacacacta aacatctgtg tgcgcactat aaaaaagagc
    95941 taaaaagcat gaaaacgcta tttcagctct tttttttatt ggcatgcttc ttgcgttata
    96001 taaaagtgta aaaaaataaa aggagtgtta aaaatggcaa aaaaaacaat catgttagtt
    96061 tgttcagcag ggatgagtac gagtttattg gtcacaaaaa tgcaaaaagc agcagaagaa
    96121 aaagggatgg aagccgatat ttttgcggtc tctgcctcag atgcagataa taatttagaa
    96181 tctaagaatg tcgatgtatt attattaggt cctcaagtac gtttcatgaa agctcaattt
    96241 gaacaaaaac ttgcgcctaa aggtattcca ttagatgtca tcaatatgca agactatggc
    96301 atgatgaacg gagaaaaagt tcttgcacaa gcagaaaatc taatgaaata aatcaagtat
    96361 gcagttgggg gtataacaac gtggaagatc aacaaaatct agaagccatc atggggttaa
    96421 tcatgtttgg cggtaatgca aagagcgatg caatggaagc gattgcggct gctaaaatcg
    96481 gtgattttga gttagcggat aggaaaatcg ctgatgcaga agaatcactt atacaagcgc
    96541 atcatgcaca aacagacatg ttgacacaag aagcacaagg aaatcatatg caagtgacat
    96601 tattgactgt tcacagtcaa gatcacttaa tgacatctat tgcgtttact gatttagcaa
    96661 aagaaatcat cgaactttat cgtcgcgtat acagcgaata aatacaactt cttttttgaa
    96721 cggaagaaga aaaatttttg aacggatttt ttattttgtt ataaggagaa ctctctttat
    96781 ataaaaaact tataggagga agtaaaaaat gaacggacta tcaagttgga tggagaaata
    96841 catcctgccg gttgccgcaa aaatcggagc gcaaaaacat ttagttgctt tgcgtgatgc
    96901 ttttatcggc atactgcccg caacaatggc gggatcagtc gctgtaatga tcaatgcaat
    96961 tattcgtgat ttaccgcaac agtttaacag tacctatgct gacagtttag cagcagacaa
    97021 aggaatcttt gccgtattga atgtcatcat cggaatcaat ggttttgtct ggaacggtac
    97081 actagcaatc gccggattga tctttgcctt ttcttggggg tataatcttg cccgagctta
    97141 tggagtaaat gatctagctg gggggatcgt tggtttagct accttgattc aaggtatttc
    97201 ctttggtcca gcattatcag cagcattaga tgtgaatgtt ccttcaaatg tcgcagatgc
    97261 aatcaatagt tctgagattg gtgcaacgat tgcagatggc acactgtcag ttggattatg
    97321 gggttggcta aaactagatc atttgaatgg aaacgcttat tttacagtta tgatcatggg
    97381 agctatttca gtcattattt tttgtaaatt gatgcttgcc aacattacga tcaaaatgcc
    97441 tgattcagta ccaccagcag tatctaaagc atttgcagcc attttaccag cgacgatcgc
    97501 tttatacgtc attgctatta tcaattatat cgtaggtcgt gtagctgatg ggcaactgat
    97561 cattgatttg gtgcaaaaat atattgcaga accattccta ggattatctc aagggttggg
    97621 agcagtattg atcgtcacag tatttgttca agtattctgg ttctttggta ttcatggatc
    97681 aaatgtattg gcaccagtgc tagagggaat ctggggacaa gcacaactaa tcaatattga
    97741 tattttccaa aaaggttata acggaaaaac tggtacacca gccgtgttag atgcaatcga
    97801 agatggaaaa gcttatatgt gggtccgcgg atcgttcgat gcttttgcat ggtttggcgg
    97861 atcaggcgga acgatcgtct tactgatagc tatcttgatt ttctctaaac gtgcagatta
    97921 tctaacagta gctaaattat ccattgggcc tggtatcttc aatatcaacg aaccagtgat
    97981 gtttggtctg ccaatcgtat tgaatgcaat catgtttatc ccattcttga ttgctccagt
    98041 agtagcaaca gcaattggat ggacagctac ttatcttggc ctagtcgctc cggtatcgca
    98101 gcaagtcaca tgggtagtac cgccgttctt attgtcattc ttagcaacag gagcggattg
    98161 gcgggcaccg atcgtaacat tagtatgtat ggtcgtgact ttcttgattt gggcaccatt
    98221 tgtcatctct gcaaacaaaa tggaccctgc cgcaggcgaa caacaataag atgaacgtga
    98281 ggagcgggac gctttaagtt tcgttcctca taatgaattt tcgaggagga actgaaatga
    98341 ttacaattgc cagtgcaatg caacaagatg aaatcaaaga attgttagaa ggatacaatg
    98401 aagaaggaat gacttttaca tttaaagaaa aacaaggaat caagctgttc tttgaaacaa
    98461 gtgcagaaga tccggaagtt gcggctaaaa aagcaaagga actaatcaaa gcacagcctt
    98521 ggggaacagt cctttatttt caatcaacag ctgtttagaa aatggtttaa aaagatagga
    98581 tggttgagat gataaaaaac ggtttatttt tatgtagtat tggttctttg atcgtgttgt
    98641 atagtttgaa tccacattcg caaaggtatg atttgtatag tatggtcaca ggtctttttc
    98701 taacaggatt aggaatctat ttcttcatga aggggaaaaa aagagaagaa gcaaaggaga
    98761 aaaataacta atggaaatca cgaaaaaatt aaatattcca gcatcgtttt tttacgataa
    98821 agttatggac tctgtccttt tcgatgtacg taaagcgacc ggcaaatcag taactagaaa
    98881 acagctgaaa aatttggagt acgtcaaaca attctcgcaa aataaccggg ctcgcataaa
    98941 aatcgaagaa gtcattgaaa accagtctta taagtttcgt acatcgacaa cgaagaatga
    99001 attcgtcgtt cattatcaaa tcaaaccatt ggacgattcg acctgtgagg ttcattacac
    99061 agaaaaaatg gagtcttatg gcatgcttca aaagttgaat gatatgcttt taggaacaat
    99121 cttgatgtat tttaaacgga gacgtttcaa aaaaatgcta gatatgatcg aagcaacgta
    99181 ttaaatcaat gaaacatggt actatttagt tattgaaaaa tgaagtaagc tagaaggaat
    99241 ttttcatagg aagatagatc tttcctgcga atcctcacta gcttttttgt aggaaggggc
    99301 agcaaaatga caaacaaagt aagagagcta ggtgtatctt cgattgagga aatgtttgat
    99361 ttagccactt atgcatttaa tggggcagat acacctgaaa ggcgagaacg ttttcgtaca
    99421 cttgcggaga attcttggaa ttacggattt tttgatgagg aggatcggct aacaagtcaa
    99481 gtcatggcga ccccttttcc agtgaatttt tacggacaag agtacctaat ggccggcatt
    99541 ggctatgttg cttcttatcc agaagcaagg ggacaaggcg ggatcaatca gatcatggaa
    99601 aagatccttg aagattgccg tgatcgtaaa gtaagcttat cttatctggc gccattctcc
    99661 tatcccttct atcggcgcta tggctatgaa caatcatttg ataaaatcag ttatcaacta
    99721 aacagtcgtg atgtgccgtt tatcaaaaaa aaatctggaa aaataaagcg tatggatttt
    99781 gaagatgcaa aagctgcaat cagaagaatt tatgaacaga tgccggaaaa ccaacgagct
    99841 ggtctgaagc gcgaagactg gtggtatacg tataaattca agcaaaaagg caattaccag
    99901 tttgctttgc attttgatga taatgaggaa gcaacgggat atatcgttta tcagcttgca
    99961 acgtcttcct ttattattgc ggagtgggga tttttggatc atgatgcttt ttgcagcctt
   100021 gttcgcttcg taagttctca caatggtgct tttgaaacat ttgaatattc atgcggctac
   100081 ggcggtaatg atcaaaatta tctattagaa aatccatttg cttctgtaat gatcacacct
   100141 tatatgatgg cgaggatcgt agatatttct ttgtttttgg aaaattatcc atttaaaaag
   100201 agacaagctg catttgccct acaaatagaa gaagatcatt atgcgaaatg gaatgaagga
   100261 atcgtggaag taacaatcga aaatggaaaa aatcatctct caaaagtaga acaaacaaaa
   100321 ctcccaatcg ttcgaggaag tatccaacat atcacgcagc tattattagg gtatcgtaaa
   100381 gtggaagagt tggtattcca agaaagaata caggtgacaa aagaatatga agaaacgtta
   100441 agctcaatct tgccacagca acagccaatt cttaatgact atttttgata aaaaacgggt
   100501 gatgacaata tttttgtcac cacccttttg tttttcattt ggcatttgaa ggaaaatgtt
   100561 aaagtaaatc ctcttttttc gtaaatttga attttttgtt ttcttttgtt ttagggtttt
   100621 cttttttgtg gtcttggatt gcttctttcg taatcttttc ccgatttttt ttagatgaat
   100681 ctaagcgttc tgcgtatact ttttcttttg gcttatcatt cattggaaag gacctcccta
   100741 cattatttca ttctacctct agtttatcat ctgttaagga ctagtggaaa cgttttgcct
   100801 aacttataag ctgatttttt tagcagataa gcttgcaagt aaaaaacaaa caagctacaa
   100861 ttgatttatt gaaattgttc ctgtagctca gtaggataga gcaatcgcct cctaagcgat
   100921 aggtcgctgg ttcaattcca gtcaggaacg tataaataac ccctgtatcc tttgcggtac
   100981 aggggtttgt tttgtatttg tgccaaaaat gtgccaaact agttatcatt tttatataaa
   101041 ctatcgaaca cagtattgat tttttcttta tcctgatctt caagctctcg aacaatatga
   101101 agataagtag acattgtagt ttctaaactg ctatgtccta aacgtttcga tacgctaagt
   101161 atatttactc cttgataaag taaaatagat gcatgtgtat gccttagtcc atgcaaagta
   101221 aattgctttt caatccctaa ttcttttaat tttttgcgca aatatttgct gacggcgttt
   101281 gatgaaacta atccattttt taagttgaaa aaaacaaaat tatgagggtt tttgatttca
   101341 aaattctcga ataattcttt ttgattgatt ttaaactttt ttaagagatc aatcgtatgt
   101401 ttatcaatgg aaattttcct atttgaagtt tcgttttttg tttttcccca ttcatttaat
   101461 ttataattcc atgttttatt aatagtgatg atttgatctt cgaaatcgat atcttcccat
   101521 gttaatccta gaagttcagc aaatcgcatt ccagtggcac cagcaactaa cacgagcata
   101581 ggagaagagt actgcgcaga aaggttttct tctacgagtt tcattaaaga tttaaattga
   101641 tcataatcta aatatttatc ttcttttggc ttcaatgaat cttttccttt gattactgct
   101701 tttctagttg gatcaaacgg aattaatcct tcttctacgg catctttcaa cgatgctcta
   101761 atatggttat taaacttaat aacggtggat tttacatggt ctttcgcata cttatttaaa
   101821 aagcgttgat atccaactct atctaaatca gaaatcaata ccgctggcat atatttcttt
   101881 atgttcatta acgtatcttc gtattttcta taagtgatag gagatactgt tccttcttta
   101941 taaagttgca tccaatcctc aaagtaatct gataataata gatttttgcg atccccttta
   102001 aggcctttgt tcaagttata ttctaattcg ttggcggcat cttttgcttc gccttttgtc
   102061 ttaaatcctg attttcttat cttcgtgtat tttccgtcat cttttttgta tgaaatttcg
   102121 tattgccaac tatttccacg tttgactaat cttgccataa ttgaatctac tctctttctc
   102181 tgatacaata ggcactataa agaagcctac tgtataggtt tgtttttctt agaacacgct
   102241 tgctttggac ggtgggcgtg ttttttattt attatacagt tgctttaaaa atagctgctt
   102301 tttcaaatga tactaaagga gagaagtgga tttctatttc tcccttttgg tttaacgtga
   102361 aatgagcagt tacatccatt tgttttcctg gagcaacaga tcccattgta ttttcgttgg
   102421 catatgtctc tgattttttg tcatctggtc catatacttc cacatctgta cctacaggga
   102481 tatctgaatc gccatcattt tttacggtat aagtaatttt tactacttgt gcaggttggt
   102541 tttcttcaaa ttgatttctt tcatcagtta gttctacact atttagtgta tattcagcat
   102601 caccaacttt tacagtgtca ccaatcttat agaaggtatc gcttttttct tctttagaag
   102661 atgaagcaga ggttgaagat ttagttactt tttcgccacc attatcactt gctttattac
   102721 tatctgatcc accatttaaa gcagaaccaa taattataat taaaattact gctaataccc
   102781 aaaaccatac ttttttgtaa aaaggtttac taactttata catttttccg tcttgaccca
   102841 taactttttt tgccatttaa atattcctca cttcttgtta taatatattt gcgatctcag
   102901 aaatgaggta tgagtccgtg ttgcagcacg ggcttttttt actgtgcata agagtatttt
   102961 ttcttgaaat atgactggca aacattaaaa cattctgttc ttaacttatt attgatagca
   103021 tagaattcca taaaattttc caatttgaac tgagattcat ctgttaattc attctcaata
   103081 aagatattaa gtagaatcat aattgcaatt ttatcagctt cagtttcgaa ttttgaatga
   103141 aaagttgtag agttatcgta cagtactgaa tattcaaaat gtgaagcaat gaaatgaccg
   103201 agctcgtggg ctaaatgaaa agcttcagaa ctatcttcgt gtagtttttc gtttaaaaat
   103261 actattcttg gttttggata ataaaaacct ggttcttcca tttccatata gattaacttt
   103321 aaattatact cactcagcat ttctttcaac tttaaataca tacaacccat cactccaact
   103381 attcattttc ctctaaagct ttagcaattg caatcgcttt acgcattgtc tccttagata
   103441 tttcttttcc atcaaaagaa aaaacagtat cgtcttctga taaatccaca tgtttagggg
   103501 tttctctttc ttctctacct agaaggtagt ctacagagac atcgaaatag tcagcaatcc
   103561 gacttaattc atctgaatta ggagtattat ttttccactt tgctagataa ccgttagaat
   103621 atccaaaatt aatttctaac tgccgtatag atatcttttt cctcttcgcc aagtctttta
   103681 ttatttcata ggtattcatc gataaaacaa cctttctaaa tgtttacaaa aaaagtttag
   103741 aaaaatacgc agaaataatt tgactaattc tgagtaatga gctatactat atcttgtaaa
   103801 cagatttaac aactaaaaag acaacaaaaa acactattga tatgtaaatg cagaccgcca
   103861 agaaagcttt aaaatcaatg tttttatgtc ttatttaatt atgctcttag tatagagtat
   103921 tactcagata ctgtcaattg aatttagaaa aaagttgtta aatttgttta cgaatataaa
   103981 agaaaggaat gagaaatatg aacacaccgc aaatttttaa tttcgaacaa aacgaagttc
   104041 ggacaatttt agtaaatgat gaaccatatt ttgtaggcaa agacgttgcg agtgttttgg
   104101 gttattcaaa cactaaagat gctttgtcgc ggcatgtaga tttggaagat aagatggggt
   104161 cgcgaatcac gacctcaggt caatcaagag agatgacaat catcaacgaa tctggtttgt
   104221 acagtttaat cttaaaatca aaacttccct ctgccaaaaa atttaaacgt tgggtaacaa
   104281 gtgaagtgtt gccagcaatt agaaaacatg gaggttatct aactccagaa aaagtagaag
   104341 aagctttgct taatccagat acaatcattc aattagcaac tcaactaaaa gaagaaagaa
   104401 ctggaagatt aatcgcagaa caaaagattg cagagtacga acctaaaatc tcctatttag
   104461 atagcatatt atcttctaca gattcagtaa caattagtca gattgcagca gattatggga
   104521 tgtctccaca acagatgaat aaattacttc ataaactagg tattcagaaa aaagtcggta
   104581 accaatggtt attgtgcaaa aaacacatga accaaggata cacaaaatct catacaactg
   104641 agatcccgaa agccgatggt ggcactaaaa ttgtaatgaa taccaaatgg acacagaaag
   104701 ggcgtctatt tatctacgaa ttactaaaaa aagaaggata ttaccctcaa atggatttag
   104761 aggaaattgg ttagaaagga gttttagtat gactgacatt gcagaaatca ctcaacgaga
   104821 tagagaaaaa atcaaagaat atgtcgaaag ttcaaagttc ttaacttaca ccatgcttgc
   104881 tgaaagattt ggaattagca aaagctactt atctttaatt ttaaacggta aaaagacttc
   104941 tgcagaagca aacagaatta tagattcgat tatcactatg tacgaattgt aaagggagga
   105001 acagctaatg caatatctag aagcaaaaat cccaattcca gaaggctatg taattatctc
   105061 ccaagtggat tatgaggagt taaaaaaagc tgatgatact ggtagatgga tgacgttgcc
   105121 agaagtgcta gaacggatta acagaaaata tgattggttt acttctagag ttttaaagaa
   105181 cccaagatat agaaatatta tcgatatcga gaaaaacaaa aatggatttg tctattatcc
   105241 agttgaagga agagacacat atctattttt aagaagtaaa acacttgaat ttttagaaac
   105301 aaatttttcg gaaatcttaa ggaggcaagc ggatgggaaa atttaataga gcgctagtgt
   105361 tcagcgcacc gctaatcatc tacgctttag aact
//
LOCUS       DASHHB010000002       100707 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.002, whole genome shotgun sequence.
ACCESSION   DASHHB010000002 DASHHB010000000
VERSION     DASHHB010000002.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 100707)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 100707)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..100707
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.002"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(552..2165)
                     /locus_tag="U2K71_000111"
     CDS             complement(552..2165)
                     /locus_tag="U2K71_000111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM7314235.1"
                     /translation="MKVVVIGGVAGGPSFATRFRRLNEAHEIIIYERGENISYASCAL
                     PYYLGGVITDRDSLIERTPEILKTKNNIDVFTKHEVTAIDPSTKRLTVKDLSTNEEIK
                     TDYDKLIISSGARPDYPDIPGVFEAENGFVLRSVTDADRIKSFLEEKNPQHVVILGAG
                     VMGLELAENFKHRGLDVTLIDQLPQVAFPYDPEIANLVYDKLLKEGLAVHLETRVTEI
                     RDKGREIVLSDGSVLSADMLIFAVGVSPKNEVVKATGIQLSDTGQIIVDDQLQTNLPD
                     IYAIGDIIETTSVVTGQPIQSMLSSAANRQGHMLADILNGTPMRYRGYIGAGVAKIFD
                     HTASYAGMTEHELKASGITNYKTVFITPFDHAYFYPEATRLNLKLIFDADSGRILGGQ
                     AFGEKGVDKRMGELSVAITGNLTVFDLPDLELPYSPPYSTTRDPLNIAGYVAINQMTN
                     IVETIKASDIPENDLKEAFFLDIREPNKAPSGSISATKNIPMNELRDRINEIPKDKKV
                     YITFRRGLNTYTSARILAGLGIKAILIEE"
     gene            complement(2306..3214)
                     /locus_tag="U2K71_000112"
     CDS             complement(2306..3214)
                     /locus_tag="U2K71_000112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7314236.1"
                     /translation="MVLLLTNNQSSNLVKKTLTYSIDTHLHLSAAPYFRLLINESANL
                     YAEFAHKSIELDNPSLLFIDKQQEVQIFSTQKETQTFIIIQFYFDEQLLAHITGQSFS
                     IDQEITSIITFLKNEQFKIDYLKHSKPAISYDQEINSFFILCQQMSIHLLSLLVTYCL
                     QKIQLSSLPRHYQQELALLTIPSNEKVIPKKKHILYSDELVDPIIRYLEEHLSENFLL
                     EQLAQIFFISPASLKKHFRKVNGKSVISYFKELKMEKAKRLVLENELTYTEIADILGF
                     HSIHHFSSAFKKYTGLSPSKYYLSLK"
     gene            3397..4521
                     /locus_tag="U2K71_000113"
     CDS             3397..4521
                     /locus_tag="U2K71_000113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM7314237.1"
                     /translation="MKKRYKLVIALLNITLLFSLSACGKTNEEVTANIADPMIKKATT
                     LEVYVANGKLTNYVENAAKIYNRKTGANIDLKIVNVASGTATVQMITPKLVTHEDMPD
                     IISMADTSAAGVLEKFQDAFYSADEYGFYDKYGDQFYDQKLNILRQQTSNETIIPWAS
                     DFTPALCYYQPKKFAEIGVDFEEISSWEEYIQVAKALKEKTGSFGIALPESGDQELFI
                     NMLAQQNQSLLDEEGNIRLNTKEAKHAAKLIKEMIDSDIVHFYGAQDAEKAFQESAMF
                     VAGGWYATNMSLNFPDASDKWRMAPLIPFSKENPGKGAVSGGSSFYVPKDAKNPQVAQ
                     QFLTFMLTDEECLANALELGVATSNSLAYQTEAAEKKFDY"
     gene            4748..5665
                     /locus_tag="U2K71_000114"
     CDS             4748..5665
                     /locus_tag="U2K71_000114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7314238.1"
                     /translation="MMQSSLPSKTIKNSKKKATDTSHKKSAYGLVTPFVIFFIVTILI
                     PIIISFAFSFMKIGYSFEFVGFDNYLAIFRDPLFIKSYTNVLIIMAGSIPITMLLAIY
                     FAVLLNSAQLKGKGFFRTVYYIPTITSTVAVASVFMTFFNPTGLFNGFLNALGINGVQ
                     WLTNPFWIRISMIITMIWLSVGYNTILFLAGLQGISKEIYESAEIDGASKFRQFFLLT
                     IPMLKPIILMATVLATINGLGNFNIPNIFFGTSNGPENSALVVGVNLYKTSFEMVDFG
                     KASAIAWTMVFVSVILSAIQFKFGGDKND"
     gene            5658..6491
                     /locus_tag="U2K71_000115"
     CDS             5658..6491
                     /locus_tag="U2K71_000115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM7314239.1"
                     /translation="MTKQKIQSRIFTYVIIVFGALIFLMPYLYMIVASTQSNQEILSG
                     GINFKFGDMLLKNWQAIMARYNYPLIIWNSIVIAVISTVLATASSTMAGYALAKYRFM
                     GNKLLFNTVMLSRMVPFFTTLIPLFYIMSRLGLSNTYAGVIIPSIASTTSVFMMRQYS
                     SQFPTALMEAARIDGANEWVIFTKIAVPILSPTIITSGLLIFMGSWNQYLFPLVMLSD
                     KDKYTVPLIIKNMTVSSTGEPINYGAIMLILATSVIPMVLIYVWAQSKFKENDIGAAN
                     K"
     gene            6574..7692
                     /locus_tag="U2K71_000116"
     CDS             6574..7692
                     /locus_tag="U2K71_000116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEM7314240.1"
                     /translation="MNEQTKEWSRKILNVASEKIDAELDRMGNKIPYISYNGKYTDVI
                     KDRGIDWWTNGFWSGILWQLFHKTKREKFKEAAIQQEQLLESALYHFSDVHHDVGFLF
                     LPSAIAHYQQSQNKHSRQLGLHAATVLAGRYNISGNYLVAWNDHKPGWMIIDSMMNLP
                     LLYWASRETGDPRFSKIARQHADTTAKYLVRKDGSTGHIAEFDAESGKYIRLLGGQGY
                     NENSCWSRGNAWALYGFALSYRYTKNPYYLAIAEKIAVNFIQKIEKTGYIPLIDFAVK
                     DSNIDTSAGVCAACGLLELAGYVSSVRSKMYHEAAINILQKTEKHYANWNMDEDGIIG
                     GGAEAYHRELTYNVPLIYSDYFFIEGLLRLEQSELAIW"
     gene            7686..9584
                     /locus_tag="U2K71_000117"
     CDS             7686..9584
                     /locus_tag="U2K71_000117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2264 domain-containing protein"
                     /protein_id="HEM7314241.1"
                     /translation="MVVKRGENTMKHPVPLLRTRQDYFDLFKKINQPLQPFYRASGAQ
                     IDYIHQGVGYGNKIAGMEGFSRVLWGAGPAIDQLDERWLKLILSGIKAGTDPSHPDYW
                     GEIHERDQRMVEMPAMLLALYHHEQLLWKKLDTGEKSQIIHWFSQIFEYECADGNWQF
                     FKIIVGTIIQKFGYPVKESALKEAFDNIENCYLQNGWYRDSSRGRQDYYNPFAFHYYG
                     LIYSVLEPEKPISQIYKNRAIDFSRDYIHFFAEDGANVPFGRSMIYRFAVSSFWSAML
                     WADLLPFKLGEIKGLVNRNIRWWMQKEIFDEKGILNIGYTYPQLTLTEPYNSTLSPLW
                     LNKIFLLLALPEDHEYWTAKEMPMPIREGTKLLSEANLLIQHDNGHTFFLNAGQLGPN
                     FHTLTNEKYLKFAYSSQFGFSIPRANQLKAEMAMDSMLGIQRADTYITTSFKNESIKT
                     YGQFIVRNNVRDIVCKKEVLASTWEPTLQAKIRTWLIPFEGWQIRVHKVELDTEYVLY
                     ETGFAIECSPEKEGIIIEEDKENYRVSEAGFSGIICLSDDTIKRKSTAIMGLPNTNLM
                     TWENTFIPGLEGTFGKGTHWLGTGVVAHQDRDYSLEVWNNRPKIDFDGTTGLTIQNKN
                     NKKRLKLC"
     gene            9578..10801
                     /locus_tag="U2K71_000118"
     CDS             9578..10801
                     /locus_tag="U2K71_000118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEM7314242.1"
                     /translation="MLKAGGEMLDKVMFEEIIHPEKWINECYKELMEKEWNNEIERCR
                     RHTERLIKRISNSQYQFPSECPIKGRYPLRDNIGWTEGFWTGINWLLFEKTGDPLFQQ
                     TAEKADIDFIHRIDCQIITNHHDLGFLYSLSTVADYKITGNRKMKEASIDAAETLLQR
                     FWEKAGVIQAWGDPSDPSQQGRMIMDCCMNLPLLYWASEETGNQKYKKAAQAHIQNAA
                     DYLVREDGSTYHTFYMDVKDGKPRYGSTHQGYSDNSCWARGQAWGIYGFALSYRYLKE
                     QKYFDLSKILANYFMNRLPEDTICYWDLIFDDSSREEKDTSSMVIMVCGLLELIESGL
                     LDKTEKNLYQSAVWKIMRQLKERYATTEEEDGFLTQSVYNKPKNNGVNTPCIWGDYYY
                     LEALVRMTSNWSSYW"
     gene            complement(10887..12821)
                     /locus_tag="U2K71_000119"
     CDS             complement(10887..12821)
                     /locus_tag="U2K71_000119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM7314243.1"
                     /translation="MILLQANQIARHFGAEVLFENIHLEISTGARVALVGRNGAGKST
                     LLKIIAGIEAPDEGKIAKNKTATLGYLAQDTGLDSNETVWNEMLKAFEQVRQMEERMR
                     ELEIFISEGTPDTPAYSSYLKEYDRLQHDFSDRNGYGYENEIRSVLHGFKFDSSFYDQ
                     KIDTLSGGQRTRLALARMLLQHPDILILDEPTNHLDIETLSWLENYLQSYTGALLIVS
                     HDRYFLDKVVNEVYELSRRKMRHYKGNYSKYLDLKAEQLASEWKAYEKQQTEIHKLED
                     FVARNLVRASTTKRAQSRRKTLEKMDRLDRPQGDEKQANFLFSIEKPSGNVVLQIEDA
                     AIGYDNVTLSEPINLDIRRQEAIALVGPNGIGKSTLLKSIIHQIPFIRGKETLGTNVT
                     IGYYDQGQADLHTNKTILAELWDEHPTTPEKEIRSALGAFLFSGEDVEKPIPLLSGGE
                     KARVALAKLAMNKENFLILDEPTNHLDIDNKEVLENALIDYDGTLLFVSHDRYFINRI
                     ATKVVELSENGSKVYLGDYDYYLEKKQEEEEIAELLAQDEPESAPAPTNKSDYYQSKE
                     QQKLIRSLQRKITQVEEEMARIDEWIDQLEQEMVAPENLEDHVKLNELNQDLEAARQD
                     QETKLTEWEELSLELEEYEN"
     gene            13047..13703
                     /locus_tag="U2K71_000120"
     CDS             13047..13703
                     /locus_tag="U2K71_000120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HEM7314244.1"
                     /translation="MKDHIIPKATAKRLPLYYRYLRILNNAGKKKVSSTELSEAVQVD
                     SATIRRDFSYFGELGKRGYGYDVEDLMHFFAKTLNDDQLTNVALIGVGNLGSALLKYK
                     FHQSNSIRISCAFDVNEEIVGRIVDGIPVYPMEDMSEQIKIQQIEVAILTIPAERAQE
                     VVNQLTEAGIKGILNFTAARLNAPSDVLIQNVDLTNELQTLIYFLHSDANAHSENTAE
                     "
     gene            complement(13777..14130)
                     /locus_tag="U2K71_000121"
     CDS             complement(13777..14130)
                     /locus_tag="U2K71_000121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEM7314245.1"
                     /translation="MKQTTNLTEVQDILQQSSVAVIYLSMPNCSVCHAVRPRLEELLT
                     HYDIPALHLDAFETPEVASKFEVLTAPVVLIFHQGKEVFRQARFIDFKKIRYLLDELT
                     AEDDSLSYEEIFRTK"
     gene            14322..15251
                     /locus_tag="U2K71_000122"
     CDS             14322..15251
                     /locus_tag="U2K71_000122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HEM7314246.1"
                     /translation="MFSFIPNSVLQMSTIFLSIVIEALPFVLLGCLISGALQVFLTPE
                     RVKRWLPENRIFSIFTGSILGFFFPSCECGIVPIVHQFVKKGVPVHTAFAFMLTAPII
                     NPIVIFSTFIAFGNSWQMASWRVIGSFFVALVIGVWLAYFQKDAVLKEKITSKVAENH
                     HHHVHEEEQSEKNEPKNQLIHQTGHVLTHSIDEFFDTGRYLIIGGLIAAAMQTYLPTR
                     IMLTLGSTKILAIIIMLLLAFTMSLCSEADAFIGSSLLSLFGTAPVAAFLVFGPMVDI
                     KNLLMMKRYFTGRFILSLIGLIALSVIGFTLFI"
     gene            15262..16098
                     /locus_tag="U2K71_000123"
     CDS             15262..16098
                     /locus_tag="U2K71_000123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03943 family protein"
                     /protein_id="HEM7314247.1"
                     /translation="MLRFLILSGYFELMMYLQVSGKLDQYINVHYRYLAILSMILSAL
                     LALVQLYLWVKNGKAEEKAHEHDHDHGLSKPYQRGIAYVLLALPVIVGTLFPTVSLDT
                     TIVEAKGFNFPVSKESVGDPEMQTQYLKPDTSIYFNKSDYLDQMNELKEKYGDKQTIK
                     ITDENYLEVMELIYNYPSEFIGKKISYEGFVYQTPNDSNTDIFLFRFGIIHCVADSGV
                     FGLLTHMPEGVTVKNDEWYKIEGTIQSDYYQPFKREIPSVVVTNAEKVSAPKNQYVYR
                     SF"
     gene            16346..17122
                     /locus_tag="U2K71_000124"
     CDS             16346..17122
                     /locus_tag="U2K71_000124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7314248.1"
                     /translation="MVTRIFILDDSLVFEKMLVEILEESRILLSPWKFEIVQGLNVIQ
                     FVEFIKNNEIYSSDIFFLDIDLNAYYSGIELGEMLREKNEKCKIVYLTSHPNKAITVI
                     NRKITPSGYLIKEFTYEKNKKSIQSYLLKEKENYPVQHATKKRWTTVRYGNSDHYIHY
                     DEILYLMSMPGYKNKIGLVLKDEEILVNGTIKKYKKSLEYEYLCKDLKAYIINTENIT
                     SISRVNEIVTFANGEQLEMGKRSIDKLKNFMQEKIENESF"
     gene            17298..18041
                     /locus_tag="U2K71_000125"
     CDS             17298..18041
                     /locus_tag="U2K71_000125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314249.1"
                     /translation="MIEFINVSKSYGKKQALKDLSLSIRQGEIFGFLGHNGAGKSTTI
                     KSLVSIIEPSSGTILVDGMKLTENRLSIKQKIGYVPDSPDIFLQLTAGEYWDLISAAY
                     ELDTQKKEKRLAELTALFDMYSHQNETIASFSHGMRQKTILIGTLLPDPDIWVLDEPL
                     QGLDPQAAFDLKEMMKAHAAKGKTVIFSTHVLDTAQQLCDKLAILKKGELIYQGAVSE
                     LLNGSPDETLEKIYLKMAGRQATGDGPYV"
     gene            18034..19614
                     /locus_tag="U2K71_000126"
     CDS             18034..19614
                     /locus_tag="U2K71_000126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HEM7314250.1"
                     /translation="MYKRQLLELIRVNLLYVNPQLTNKAREKGKKGKRLTIYLLSQNL
                     FSGLIFLLIYGFAMFAMNFVKLPGFFTYYVALFGMLGLTQSISVIYNIFFEGNDLQVF
                     LPLPFGQEQVFSAKILVVALTIIPFVFPLLVMFILTGWRADLGILLTIIISILLFSLF
                     LIILFCLASLIVFGLARTAVFKKHRKTVSSILLGSSMLIAFAGIMWMNHQTGTIENEL
                     TDRHPISILLPFYDAASQPLSQQGLLGWGILAITALGFFLAVKQLIVPKLTEQLTDSN
                     SEFKIKRTHKKNQNVHQLLFSYNAQLVKEPNLIMQVLSSSLLTPIIFILAFALGGEIK
                     LTDLDNKLIGVVFLVGIALAFLTVNQTSFISNLISLDQENFLFIRSLPISMNQYLKEK
                     FRFGWILQSFLTGGIALLSGLSFQLPLFFIPSLLFGSLFGCYLLSLRYFARDYRLLLL
                     NWTNINQLFNRGSGSLGLVATMMGSLIISIILIVLYGMLALFYPFWLVNIPVIGLVLI
                     LSALWIVYYQRTFWKKFE"
     gene            complement(19865..20344)
                     /locus_tag="U2K71_000127"
     CDS             complement(19865..20344)
                     /locus_tag="U2K71_000127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEM7314251.1"
                     /translation="MGTIFIQFSFSFLATAAFAVITNVPRRSLICCGLSGAFGWMIYW
                     ICVQIGGSAAFGSLLGSLGVAFISDLFSKRLKMPVTIFNIPGMVPLVPGGLAYQAVRN
                     LVTESYREAAHYAVQAIMIAGAIALGLVLSEVFNHNIRNFKVKRDAIMMKRKKRLFK"
     gene            complement(20360..21112)
                     /locus_tag="U2K71_000128"
     CDS             complement(20360..21112)
                     /locus_tag="U2K71_000128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine/serine exporter family protein"
                     /protein_id="HEM7314252.1"
                     /translation="MTGKELDLLLDTCLLAGKIMMESNAEMYRVEDTMSRIALASGNF
                     RLVSYVTQTGLFIGLDRTSTIRMEQITNRSINLEKVVKVNDLSRKYVASELTLEELYC
                     ELQAIDRDRSFFPVWLQIVSAAVISGTIMVLFGGKMTDLPVTLVIGGLGYAAYLYSLK
                     FFRIKFLSEFLSSLLIGIAAIYSVRFGLGSNQDLIIIGCVMPLVPGVQITNAIRDLLA
                     GHYVSGVSRGTEAMMTATMIGFAIAFIFQLFY"
     gene            21229..21299
                     /locus_tag="U2K71_000129"
     tRNA            21229..21299
                     /locus_tag="U2K71_000129"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:21261..21263,aa:Gly,seq:ccc)
     gene            21660..22385
                     /locus_tag="U2K71_000130"
     CDS             21660..22385
                     /locus_tag="U2K71_000130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA/RNA non-specific endonuclease"
                     /protein_id="HEM7314253.1"
                     /translation="MSKKTTKAIVGIVAAVIAGAFGVSTTQKDSDIFQQITTIFDSTP
                     QASQVADVPEYDGKHQEIEINGNQPEFTKEDLSLSKGTWESYSNIDKLNRVGQANAML
                     GKDLFPTEKREPLYIDPTGWKQKKLSDGQWLYNRSHLIGFQLTGQNNNIKNLMTGTRS
                     LNAPYMLAHENDIAAYIKETNHHVRYRVTPHFEGNELVARGVQLEAESIEDKKIEFNV
                     FIYNVQDGYTINYETGQATKTKN"
     gene            22640..22906
                     /locus_tag="U2K71_000131"
     CDS             22640..22906
                     /locus_tag="U2K71_000131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IreB family regulatory phosphoprotein"
                     /protein_id="HEM7314254.1"
                     /translation="MGFTDETVRFGFDDSRKKEVSETLAIVYRALEEKGYNPINQIVG
                     YLLSGDPSYIPRYRDARNLIRRHERDEIMEVIVKDYLSNHGVNL"
     gene            22903..23334
                     /gene="ruvX"
                     /locus_tag="U2K71_000132"
     CDS             22903..23334
                     /gene="ruvX"
                     /locus_tag="U2K71_000132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RuvX"
                     /protein_id="HEM7314255.1"
                     /translation="MRVMGLDVGSKTVGVAISDPFGWTAQGVEIIRINEAEEEFGFDR
                     LKELVAQYEVDRFVVGLPKNMNNSIGPRAEASMSYGEKIKELFSLPVDYQDERLTTVQ
                     AERMLVEQANTSRAKRKKVIDKLAAVMILQNYLDGSGKQLF"
     gene            23359..23664
                     /locus_tag="U2K71_000133"
     CDS             23359..23664
                     /locus_tag="U2K71_000133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002419177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1292 domain-containing protein"
                     /protein_id="HEM7314256.1"
                     /translation="MTEHNHSHDHEGHEHITLVDDQGNETLYEILLTVDGQEEFGRNY
                     VLLYPAGVSEDEDVELQAYAYIENEDGTEGELEQIETDAEWDMIEEVFNTFMAEEEE"
     gene            complement(23745..24890)
                     /locus_tag="U2K71_000134"
     CDS             complement(23745..24890)
                     /locus_tag="U2K71_000134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM7314257.1"
                     /translation="MATFKQYETKKGKFWLYEAYLGMNKMTGKPDKVRRRGYKTKKEA
                     QLALSRLQVDYDKNGIKKANNETFKEVYDLWLETYKTTVREATFMKTEIKFRKWILPK
                     YGNLRVNEVTVKKAQQIVNSWAETTDQYKVLHSTSKRIFSYAINLGIIETNPLDNVLM
                     PKRTTTKHKEKVKVYSKEQLITLFDYLNSKPETYRNDFDKTLLRFLFYSGVRISEALA
                     LNWSDIDFQENTVTINKTLSQSKNGYKISDPKTDCSAGVLPLDERTISILKKWQINQR
                     KYMLTLGVTDPTMIFCGIYKELITHHAIYARLITICEKAMVPFLGVHVARHTHASMLL
                     DAGATMVEVQTRLRHTKISQTIDCYGHLAKETKEKTVEKLVQHLNID"
     gene            complement(24951..25598)
                     /locus_tag="U2K71_000135"
     CDS             complement(24951..25598)
                     /locus_tag="U2K71_000135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7314258.1"
                     /translation="MNGGENVARKKYPEAVETGQRIRLARIEKGLSMEQLGGKLSPPA
                     SKGAVSNWENGYNLPNNTRLKQLSKVLDVSTTYLLNGSYTLRDMHMMPEIKRNVLEKQ
                     LKKTIEKNNSIIDSNLSNAINNIEEDNLTVSQKIYLTNAIKYLKTGSRIEFPDGSTPI
                     AYLAALLNTLLRYDSLINDSEKNTDEKKEYQEFYENDLLKEFTALVTELNKHYSK"
     gene            25792..26085
                     /locus_tag="U2K71_000136"
     CDS             25792..26085
                     /locus_tag="U2K71_000136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314259.1"
                     /translation="MELKIDKLTLDDPAFKIIDDRVVQQVEQTQGTLLSLLEKKYQFP
                     KYMNKAQAAKYMNVSYNTMMQKYVPNGLKLIIVDGVVRISQDECDRFMEEYQK"
     gene            26129..26440
                     /locus_tag="U2K71_000137"
     CDS             26129..26440
                     /locus_tag="U2K71_000137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314260.1"
                     /translation="MESTQNKAIEKVLSTILTEDTAAELANLEGKALEETFECLYEQM
                     DYQKLLPQEPTVSGVLRGLNDLVQAEFKERLSIEEYQEILYQQVELLASLLGLELEDV
                     E"
     gene            26437..27061
                     /locus_tag="U2K71_000138"
                     /pseudo
     CDS             26437..27061
                     /locus_tag="U2K71_000138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350005.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phage repressor protein"
     gene            27202..27360
                     /locus_tag="U2K71_000139"
     CDS             27202..27360
                     /locus_tag="U2K71_000139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314261.1"
                     /translation="MKRNLIIGSILIGGIFGLLPLIAKIAIFAGLALYLFTAYDEYDY
                     QLRTGGHK"
     gene            27361..27723
                     /locus_tag="U2K71_000140"
     CDS             27361..27723
                     /locus_tag="U2K71_000140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314262.1"
                     /translation="MYKTKLLNQLDSLELEEINQGIAELENNIGKTYFGNSFNEKLTV
                     LYVLKKHAEHKIICREINELKNQILTAWLNITDMQEARVKTFNTWVKYQNQLKGAEFV
                     RDGLKYELEQLKLMEVSE"
     gene            27760..28608
                     /locus_tag="U2K71_000141"
     CDS             27760..28608
                     /locus_tag="U2K71_000141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA primase/polymerase"
                     /protein_id="HEM7314263.1"
                     /translation="MANNYQAATELQKSGWSIYPLSPGTKIPIKGSSGWNDATNDSNQ
                     INEWWTEDATRNIGLMLEDHQMVVVDLDQHSETRNGVENYKKLASMYDPFPSTYTEIT
                     PGKGIHFFFKIPDGVIIQQETGAFADIFGRDDKGKSLSGIDIITKGVPIAPTMIISRS
                     VGRAYEPIENVSLNNIAPAPDWLVNLLLKKPQEIKTQYRPVAKTGTAKKLDMIVQGAG
                     EGSRNDHLTKLCGWLLWHGVDNQTLAELIYTANAYNEPPLKDKEVNQIIRSMIKKDLR
                     GKAHGS"
     gene            28598..30073
                     /locus_tag="U2K71_000142"
     CDS             28598..30073
                     /locus_tag="U2K71_000142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase"
                     /protein_id="HEM7314264.1"
                     /translation="MAHKHKLDSILDFPEASEREDNIIELKTWMSRLRCNKDDQIKSN
                     SVVNAELILTNDSNLAGTIAYNEFSGYIHLLKDSPWINRTAGEWEDSFEDALTAYIEE
                     NYNVVFDDNKIHKAVVNVARKNVFNPVKERIEKVKWDQQPRLETLFIDLLGVDDNLYT
                     REVTKRWIVGSVARIYKPGIKFEIVPVLDGPQGIGKSTVPALLYTDDFFTDSLDSLGE
                     KKDDYMQLQGNVIIELGELSSMNKTKIEQIKNFISAKIDKIRPPYGRNVIAWARQCVF
                     IGTSNDGQYLKDDTGNRRFYPLPCKNKPKMNPFKTKDEYFLQVLAEAKVLFDKGQRIY
                     FSPDEDKEVLEIAKDYQEDAKMENPIKEAITKYLEMEIPYKWEEAPAWARRSYYQNYP
                     DSKCNEKNLQHFYNGATMQQEFYLVDSVLTADILEAVFDKQAKDLLNGRSDAETKKIA
                     LIISSIPGWERKQLPKRNKRRGFYNKSNAYENKNRAGKYKK"
     gene            30339..30746
                     /locus_tag="U2K71_000143"
     CDS             30339..30746
                     /locus_tag="U2K71_000143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3206 domain-containing protein"
                     /protein_id="HEM7314265.1"
                     /translation="MLNIISTNKAPNFQYTDEMDRFLMNTLAFSVGLVTEDYSTFDPE
                     VLKIMEEEPDWLQESVAWCQSLVVGSLVDSGNYDDTGELMDEFNCLLNLYDRARQREL
                     TSNEDNLFLNIHDKFLALLLTDDELITNLLEVE"
     gene            30749..30964
                     /locus_tag="U2K71_000144"
     CDS             30749..30964
                     /locus_tag="U2K71_000144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314266.1"
                     /translation="MHMESIRYFDGEKHIEYSNTQEDVDFISFEEDKITKVNFKDGTC
                     LKIVSPYIEYKSKWEQDDSDERDRFGW"
     gene            30968..31348
                     /locus_tag="U2K71_000145"
     CDS             30968..31348
                     /locus_tag="U2K71_000145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEM7314267.1"
                     /translation="MAVKKQCNHAGCKTLIDYRQKYCGKHKAKQTAIKREERKQSEGK
                     YFQFYQSRTWRKASYLYRLNHPVCEDCLEEGLIRKADVVDHKIELKDDWNRRLDETNF
                     RSLCHAHHNSKTANERQRREKSTL"
     gene            31482..31637
                     /locus_tag="U2K71_000146"
     CDS             31482..31637
                     /locus_tag="U2K71_000146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2292 domain-containing protein"
                     /protein_id="HEM7314268.1"
                     /translation="MKVGDLLKNTIVITDKTGKQRTIELPHFGQVTIQMQNGKIIYID
                     KLDKEKF"
     gene            31706..32179
                     /locus_tag="U2K71_000147"
     CDS             31706..32179
                     /locus_tag="U2K71_000147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage terminase small subunit P27 family"
                     /protein_id="HEM7314269.1"
                     /translation="MGHPKILHDTKGNISSEEKAIRVDAREELFKQQPLINITPPDWM
                     AASARSEWNRIVPTLKKDYPLSEADYGSLVAYCLAFARMKTAEAEIRKSGTFITCENG
                     VKKANPAVRVQSQAMSDLKKQATSLGMTLESRSKLALNKVKNDEPEDPFKELMNS"
     gene            32176..33870
                     /locus_tag="U2K71_000148"
     CDS             32176..33870
                     /locus_tag="U2K71_000148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEM7314270.1"
                     /translation="MNDYIEKVLSGKLIAPKKIIQACERHISDLERSKSDSFPYVFDE
                     EQATKAIKFIELLPSTDGKAIKMLGFQKFILGSLYGWRTKEGNYRRFNRAFTSMSRKN
                     GKTYIASGMAANALIMEQEPAEARQVLFVSNALKQAKLGYDMLSNSLRNVVKSSKFLR
                     PQLKIMNSKIQHLPSNSFAMALASETSTLDGFAPTTAILDEWHEAKTRKTYNVIKSGM
                     TQQKNGLLCVISTAGLDLNVPMYEEYLLLDRVLKGEEQADRYFIAIWELDDPEEIHDQ
                     EKWIKANPIFESEEIKKVMIPTIQDDVNLALKQNNLNSVLVKNFNLWRQASEDSYMIA
                     EDWQATEVEPQDITGKPVYIGVDLSKTDDLTSVSWIVPLDNGELYCDSHSFVATKYGL
                     QDKEKRDGLPYRELEKAGECSITQLESGIVDYDQVFQFIQDLIQENDLECMGICYDPY
                     NANSLISKAEKANYPMLEVRQGTITLNVPTRTFREQVYEGNVIHNKNTILTHAVNNAI
                     LKTDNNGIQINKSKNSNKIDPIAALINAYVFAMDYFTTTEGAKADNEFYTSEEFSF"
     gene            33836..34021
                     /locus_tag="U2K71_000149"
     CDS             33836..34021
                     /locus_tag="U2K71_000149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314271.1"
                     /translation="MNFIQVKNFLSNYIHTVLLLLGLVCVLVAITLLTNVYYGLLALG
                     IVLIGVAVMLNTEQKGG"
     gene            34025..35200
                     /locus_tag="U2K71_000150"
     CDS             34025..35200
                     /locus_tag="U2K71_000150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM7314272.1"
                     /translation="MAFFKARQNTTGDPFLQHVVSIQSDDYTTSFTSVRALRNSDVFA
                     AVRIIASDIASSPIQLVKNNMPQADNELVKLLNEKPNSEMDGWHLKFALAVNMLLNGN
                     SFAEIKRNGEKVEEIHLLPNSSVTVTQLDNGTLSYQIGDKKRRVKSSDILHFKYFTQD
                     GLTGLPPLYALRDELKIQQAGNRTLHNFFTRGVSGSGILKVHKSDLDGSAKNAIREKF
                     EEANGSSSGDNALRTIILDETMDYKTLEINTDVLKLINSNDWNTKQIAKAFGVPIERL
                     GVENEHSSTVQSNLQYIQSTLIHYFNVFVSELDTKLETNIRFNSDQLLETDPETKVKN
                     VLDQVKGSLLTINEGRSKMGLPPMDGGDRLLASLNFTYLDTLEKYQLKEQEGVTPVE"
     gene            35193..36716
                     /locus_tag="U2K71_000151"
     CDS             35193..36716
                     /locus_tag="U2K71_000151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEM7314273.1"
                     /translation="MNNEEEKEKRLTEEAELTADSPKVEKENEKQPTDGKTISGYALK
                     FGQPSKDLGGFVEVITPEALKEVDLSNVFLLYGHDYSKPLASVKAGTLKLNVDDTGLH
                     FEAELTDTTYSNDVYENISKGVVDSMSFGFVLGLDSFDKKEDGTIERSIDKIKALNEI
                     SVVTVPAYDSSNVQVNKRSYESFMSNNQAKQTNNSLESTSKAQKESNNMEKTLIDNEK
                     TELRGYEEYIRSQGEVRDGVTTVNAAAVVPEEVIGEVFDLKRSNYNLAQYATVKTVSN
                     GQGKYPVATNQQAVLATKAELAEIGDIDAEMFTSVDYKVETRAGKIALSNEVVEDSAV
                     NIVQEVKDQLAKLVENTDNKHIMDLLKTFTKKTAATLDDLKQLYNVALDPALNKMVIL
                     NQSGYYHLDTLKDSDGRYILQPDVTAPSGKSLFGMPVVLIADTLFANPKAGTFPMIMG
                     DIAQSIFVARRNQVTTQWEKFDYYSQGLAVIVRNDYKKIDQNASVYIEFTPVVTPKV"
     gene            36772..37056
                     /locus_tag="U2K71_000152"
     CDS             36772..37056
                     /locus_tag="U2K71_000152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage gp6-like head-tail connector protein"
                     /protein_id="HEM7314274.1"
                     /translation="MVILDSIKKSMRIDHTIDDDFIQQLIDTAGEYIKSAIDSSATDK
                     DMDNYQQFDLAVSLLTQHWYLNRQEASSERIPVTVQALVQQMRGAYYANH"
     gene            37043..37378
                     /locus_tag="U2K71_000153"
     CDS             37043..37378
                     /locus_tag="U2K71_000153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEM7314275.1"
                     /translation="MPIIKNVNELTERVKFKKTKRVKDEDGQMVDSEETVFECWANVR
                     SQMLKDVLASVGTILEGTLTFIIRYDQDYELTNDMKVAWKNKSYKIISINEGTAFKDY
                     TTIIAKQIS"
     gene            37673..38299
                     /locus_tag="U2K71_000154"
     CDS             37673..38299
                     /locus_tag="U2K71_000154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314276.1"
                     /translation="MDKAEFTNFYWKYYLHLENEFINTTDFVMLDENNFKTFSIEYQK
                     LLLAIGSECEIIFKELCGFDSSSNKSITDFKAVIKSSELMCLDNGVRVMNSLTLTSLK
                     PFGDGWPEDTPDWWKIYNRVKHGRSSNYKEANLENVLYALASLYLLEEYLHKKVILEG
                     EVDFISPESNLFTLSWERKHSSMQKVTFEKVDSNYVAPLIDFKEQEEI"
     gene            39041..39877
                     /locus_tag="U2K71_000155"
     CDS             39041..39877
                     /locus_tag="U2K71_000155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HEM7314277.1"
                     /translation="MIGLIYFVVIVLANTVGAISGMGGGVLIKPILDLIGAHSVAGIS
                     FYSTVAVFTMSIVSTVRQVSSGKSLNWQIVGWVSSGAVVGGVVGNIVFEVFLQLFENE
                     KHVQLIQIFLTVLTLVFAFFYTKHHQPKFHLTSWTWYLICGGVLGFLASFLGIGGGPV
                     NVSLLMLMFALPIKEATLYSLSTIFFSQLAKLVTIALTSSFMRFDLSMLFYVIPAAVI
                     GGLWGARFSRVLSPKKVTFIFQVIVLVVLLVNCYNAFVILQTLGESRLTQVKITETYC
                     SK"
     gene            39941..40372
                     /locus_tag="U2K71_000156"
     CDS             39941..40372
                     /locus_tag="U2K71_000156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7314278.1"
                     /translation="MLREMRLGDAEALREINAEQLGYDVPLTVTVRQMKKLLKEPEKN
                     FFLVYEDPTSKKAAGYVQAESYESIFSDPMFNIMALAVSKHAEKRGIGKALMTGVEEA
                     KRRGISAIRLNSAEYRVEAHRFYERIGYHSDKMQKRFLKIL"
     gene            complement(40434..41756)
                     /locus_tag="U2K71_000157"
     CDS             complement(40434..41756)
                     /locus_tag="U2K71_000157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxycarboxylate transporter family protein"
                     /protein_id="HEM7314279.1"
                     /translation="MAQKTTTATAVEEKKKEGFWSTKITGVSLPLYLIMVLVLIIAMA
                     LGKLPTNMIGPIAVLVIFGNLFHFIGTKIPIVKSYLGGGSVFAIFASAAIATFGILPE
                     YVVKSTENFVSNMGFLDFYIAALITGSILGMNRNLLMKASVRFIPVALISMVVSFFAV
                     GLVGMLIGNGFANSVLYISLPAMAGGVGAGAVPLSTIYANVLGTDASSIISRLIPATA
                     MTNVLAIIGAALVARAGQSMPKFNGNGKLMEKGDLGDGKPRNVKPSIPQLGVGLMVSL
                     TFFILGQICNYFVPKVHAYAFMIIIVVICKIANLLPEYYEDAAIMFNNLIVKNLTAAV
                     LAGIGIALLNLNVLASALTWQFVVLCLTSVIAISVVSGFVGRLFGLYPIESTITAGLC
                     NNSMGGTGNVAVLSAANRMELIAFAQMGNRLGGAIVLIISGFLMQLLS"
     gene            complement(41910..43550)
                     /locus_tag="U2K71_000158"
     CDS             complement(41910..43550)
                     /locus_tag="U2K71_000158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent malic enzyme"
                     /protein_id="HEM7314280.1"
                     /translation="MLTGLNLLNNPFLNKGTAFTKEERAKYGITGMLPSTVQTLEQQS
                     VQAYGQYLSKQTDLEKRIFLMNLFNTNRTLFYKLMGEHLVEFMPVVYDPVVADSIEQY
                     NEIFLKPQDAAFLSIDEPENIKASLKNAADGRDIRLIVVTDAEGILGMGDWGVNGVDI
                     AIGKLMVYTAAAGINPAQVLPVSIDAGTNNEELLNNPLYLGNRHARVEGETYYEFIDQ
                     FVQSATELFPELLLHWEDFGRGNAATILEKYEDKITTFNDDIQGTGIVVLAGVLGGLN
                     ISGEQLKDQTILTFGAGTAGVGIANILLDEMIRQGVPEEEARQHFYQVDKQGLLFEDT
                     KGLTPGQIPFARKRSEFTNADELTNLEAVVKAIHPTIMIGTSTQPGAFTEEIVKEMAA
                     HTPRPIIMPLSNPTKLAEAKAKDLIEWTDGKALVGTGIPAADVEYNGVTYQIGQANNA
                     LMYPGLGLGLIASTATRVNAEIISQASRALGGIVDVTKPGAAILPPVAKITEFSQTIA
                     ETVAKSVVAQKLNREEITDIKEAVESAKWVPEYKSLED"
     gene            43883..44353
                     /locus_tag="U2K71_000159"
     CDS             43883..44353
                     /locus_tag="U2K71_000159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4809 family protein"
                     /protein_id="HEM7314281.1"
                     /translation="MKEVQLIRKSELSEGGCNACGVVEATSYTLKLGANKAIISELTV
                     GGLVDSLALAEGFIGEDIYEMFSEIRQLKKGENCIEVHHESPNVRFKRGDNEMIFNNH
                     VSDHTELYGIVNQILTELFGLGPYAFKEENGNPKLNEEWQETIEIQRNNPHLFQ"
     gene            44575..45678
                     /locus_tag="U2K71_000160"
     CDS             44575..45678
                     /locus_tag="U2K71_000160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEM7314282.1"
                     /translation="MEKTYQAGTNEGIVDFINMEDLEIAASQVIPAGGYGYISSGAGD
                     LFTYQENERAFNHQLIIPHVLKDVELPDTTTHFDKETLTAPIIMAPVAAHGLAHVKAE
                     KASAKGVADFGTIYTASSYASCTLEEIREAGGEKAPQWFQFYMSKDDGINLDILEVAK
                     RNGAKAIVLTADATVGGNRETDRRNGFTFPLPMPIVQAYQSGVGQTMDAVYKSSKQKL
                     SPKDVEFIAAHSDLPVYVKGVQSEEDVYRSLESGAGGIWVSNHGGRQLDGGPAAFDSL
                     QYVAEAVDKRVPIVFDSGVRRGQHVFKAIASGADLVAIGRPVIYGLSLGGSTGVHQVF
                     DFFKTELEMVMQLAGTQTVEDIKKIKLRENRFI"
     gene            45962..46642
                     /locus_tag="U2K71_000161"
     CDS             45962..46642
                     /locus_tag="U2K71_000161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEM7314283.1"
                     /translation="MKKNMIGTLLLSTLLLGGLATPAFAEGQATSKGDITFTEPTNTV
                     EPLNPTDPSKPVEPADPENPATGQTGPLTLDVVPELPFGTHEIESGTKTYQVDASKND
                     TPYLQVSDRRGVGADGQAQGWNVTVSVSDFVNGSQVLQGAELDFGTSTVKSTSDNEST
                     GPTSQTVTGLSKASAATPIFTAAKDQGLGTWLSVYDPANITLKVPKAAAGTFTADLTW
                     NLVAGPVA"
     gene            46732..47766
                     /locus_tag="U2K71_000162"
     CDS             46732..47766
                     /locus_tag="U2K71_000162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEM7314284.1"
                     /translation="MNQSKRLFVSFFSILSLFFIFPSISQAKDSAGDFGIKPVFPENQ
                     IDKAIGYFDLLVAPEQNQTLEVIISNSSDEERTFEVSVNPAVTSDGGTIDYSQKNPTL
                     DETLPFDVRDVLLIAKKEINVSAHAETTVPIEVKIPAKSFKGRVLAGIHVSPKEEAET
                     ENATEGAQIKNRIAYNLAVVLQESQETIEPDLKLLSGDLDEVNAKPTVQLRFQNPQPR
                     IISNLIFTSKIFYENQLYIENTSNAFLVAPNSNFHLNLDLAGDKAKAGDYRAEIIAKS
                     GDSNEWRFTQNFTIKKEKAQKVNENSVFAVQEQSFPWLYAGLVAAVLVLWLILLFLWV
                     HKKKKKGETT"
     gene            47780..48115
                     /locus_tag="U2K71_000163"
     CDS             47780..48115
                     /locus_tag="U2K71_000163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314285.1"
                     /translation="MKTSILLGGIIASLLFFSWQEPVQADTKSSGTITFYDLSPIPHP
                     SGSVNGASKQQTQLPIYKESNQHGVFPETNEQKIRFLYLIIGIELWLLAWLLLWRRKE
                     KEDEHVATK"
     gene            48099..50579
                     /locus_tag="U2K71_000164"
     CDS             48099..50579
                     /locus_tag="U2K71_000164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314286.1"
                     /translation="MLLQNKFSFFRSFFFTSLFVLSIFIFSPSAEAVGATPPAISYDP
                     YTYENIVPPNGGRSAVNGGKLVQLTGANQLPKLTATYLNDGSNDPWKGGTVRFHWESH
                     VKLTNVLDLVNITAQGFAFVAIINLPEHVSSSDVLKAIDWNNAYLYIAGAPIKVTSGS
                     LLSAGSHTLRFGMGSWDTNTLLTDIIAIITRGEFNVDDIPLQFDVTANVAKMTENGDK
                     YDTSPNKILTKGKLQPSMSKKAVFSVDFYDSNNIIQDRMFNSILAPAAGRFYPKLTGG
                     NLNPGAYAFSASAEINTWNSYISPSDSTGKYNTFADTTEYVDGNNQNLPGSTNSRTLT
                     MGLTEGRFDQLPFSRFDRVVNYFTGKNVTQGAILTHTPASTSGLNQTANIVYSGKDAQ
                     GGLLSPVALKVLEQYKLDGTVLPTNLTADSQWGKLLSSASYQVTANWSATELKKGSIH
                     YRLYSKGTQKLATGWTDQLFQTIQSNNGGVNTATSTLPALASGQYYFDYRLVDDVLTN
                     MYPSFAYKWQSEQAGITTLPQITVANFPSVSADSQLKNLSRQPETGDPLSALSGDTIQ
                     ENVTFTLTKSGDAITDKKITISLPENTTYINGSLKLNGTTIADTGIQQGVSVPADLLS
                     KIGDTIHLTYNYQLNTVDTSLQSVSILTKAAVLSSNITLADGAKLPNPVVQTSAKTIL
                     VPKQELTLVNVPDDFTFGNDLPKPLKTSYYESKGDFSFDVRDTRLPSTSPWQLTGTLT
                     SLFKNDQGQELSGTKLYFNHSGSKQLIQQGQNTLIYESDGTAKGEVLVDFPDTDGLLL
                     EVNSSTNAQPGATYQGMVTWKLTAGPTS"
     gene            50635..52194
                     /locus_tag="U2K71_000165"
     CDS             50635..52194
                     /locus_tag="U2K71_000165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7314287.1"
                     /translation="MRRKLLLLKSEMLEGVDLELFSLLEKNEQRQIKLFQLLLEEKGE
                     VSVNNVLTRLSVDRATLKEDLFYLQANLENIKQLEIVIQEGNILLIRHGNIAAVDVYY
                     HCYLRYSTKYQILLYLLENGSFDRQKLVMELNISSATLSRRVKELNDSLNEFHLKIKS
                     GRLTGPESQIRYFYFQLFWFGRPYLENKKEYSDLSGNTFLDLLQKEFSFAITEEGRLK
                     FEIWLSIMKKRMRCTKKSKPDYSNLPVGLFQTTQFLLDLHTFLTRYFFQYAFPWNQNE
                     TLVFYLFMISNFTLDTHNSHVQEFLISEKYKDDVVAQLNRTFKQSIEEYFSFYVFPEE
                     ISQKIALTVMQNHAHFAYFRGWMNIFGQEGLKQRLTEVEDKQFLVLCERLVRQTFAIV
                     DFTEVDEENARFELFARYASILRTLIKQLELRLQIAYDLPYERLMADIVKEIIQEQLL
                     NTIKYDLVEYEKEKQYDIILTSQLKEYPSQKSAKVFVFTSNEYNYDFPYLNQFLKECY
                     LEKLNLRMNKT"
     gene            52191..53555
                     /locus_tag="U2K71_000166"
     CDS             52191..53555
                     /locus_tag="U2K71_000166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM7314288.1"
                     /translation="MKIVIIGASHAGITAALNLRKLQPKAEVLLIDEDHKDGLGYVSN
                     GINLYLKGKIRSLAEVAHNMRTLQESGAKLITEWRVTELDPDKHQLILASKEGKNETI
                     TYDKLIVATGSSPVTLYKQIEAENVYTYKNLVQSKQVLAALKEAKEVVIFGAGYIGLE
                     LADALRNKGYIIHLVDYMPNVLSRYFDKDMINSFQNQLQTKQINFYPNEFLIDWKKSE
                     EKVVSVQLLSQTIKADMVIFSAQTRPNTTLLKEKVALYEDETVIVNEYLQTSDPDIYA
                     IGDIVPVSFDKNKRHLFLPLVTRAVHMARAVALTLSGQPTAYDLRQKITAAVIIDYFL
                     GTVGLTEDEAPFLEQNTGSCSGEFDLFPQYDEENKTVNAKLIYHPDTLEIIGGQLISQ
                     EFLLSDLNLLADIVKHKTTIPQLAVEDFGFLSEYTSRFHYLNELAFKVLAKRANSNRV
                     DKRL"
     gene            53726..54796
                     /locus_tag="U2K71_000167"
     CDS             53726..54796
                     /locus_tag="U2K71_000167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314289.1"
                     /translation="MFLALNEIMHSKLRYALVAGVMFLIAYLVFFLTGLAYGLAQDNR
                     TAVDKWEADSIVLSKDANSNLGMSMITKKIAEEVEGGKVAYLAQTPGVVTSKDSTEEG
                     KINVSFFGIDKNQFIMPNLVEGKAFDNDDEAVGDISLKEEYGLAIGDTVKLSGSDKTF
                     KLTGFTDHAKFNVSPVLYTTINAYQEIRFEKEDTSENARINAIVVRGKISNLPEDLEQ
                     IKISKFINELPGYNAQVLTFGFMIGFLIVIAAIVIGIFIYVLTMQKINIFGVMKAQGI
                     TGGFIARSVVAQTFILSFVGILLGLLGTVGTSLVLPDAVPFQSNWLFFGVISLLMLVV
                     AVLGALFSVRTIVKIDPLKAIG"
     gene            54811..55479
                     /locus_tag="U2K71_000168"
     CDS             54811..55479
                     /locus_tag="U2K71_000168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314290.1"
                     /translation="MKAIEFKEVEKSFQDGDQVVKALKETTMSIDKGEFVAIIGPSGS
                     GKSTFLTLAGGLQTPSSGSVFINDQEFSNQKESERVKLRFKEIGFILQASNLVPFLTV
                     KKQLLLVDKIKHEHRQKEANELFEQLGVDKLINKYPEELSGGERQRVAIARALYNDPS
                     IILADEPTASLDSEKAREVVDILAKESKDKNKATIMVTHDTRLIDKCDRIFMMEDGVL
                     KEKE"
     gene            55499..56077
                     /locus_tag="U2K71_000169"
     CDS             55499..56077
                     /locus_tag="U2K71_000169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7314291.1"
                     /translation="MVHRYNKDTQQKILKEATDLFMSKGYLGTSTREIAQKVGITQPN
                     LYHYFGDKEKLYTAVLENHLKDVGKALREIVQTSETGFQVTLTKMAQFLIETHLVDLF
                     LMLHDLESNLSKETRDHLFFLWKKNYREPFEEIFSANQSVLRNGISKEIAARHFFLVL
                     APYITRSKRPVEEPLNVAQLIDLYLNGVLGEA"
     gene            56478..57389
                     /locus_tag="U2K71_000170"
     CDS             56478..57389
                     /locus_tag="U2K71_000170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEM7314292.1"
                     /translation="MKKIIGAFVFAIIIIIICFQIDVFGQKKIVGQTVSSTNSTVTKT
                     KESFDDTEQETLIKIGKNDRTLYTDDSLTTPLATINGGELAEFLSETKNAYQIKTNDG
                     YTGYLALEDGTKVTKNIQTKPEELSDAVIVLDPGHGGNDTGALSNDEQTEEKDITLST
                     AIKVKKALEDAGATVYLTHTTDELVQLGDICDYSEEKKADVFISLHADSTEYANEATG
                     ITTYYYYGQEETLAQTIADSFTDLPLDSRGISTGNYQVLRENLQPSILIEMGYMNNDS
                     DLKELVTDSYQQQIAASLTQALTTYFQ"
     gene            complement(57477..58394)
                     /locus_tag="U2K71_000171"
     CDS             complement(57477..58394)
                     /locus_tag="U2K71_000171"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HIII"
                     /protein_id="HEM7314293.1"
                     /translation="MSNVIIKATNDQLSQMKAYYRSYLLNKQIPYTSFAAKKDGTTIT
                     AYTSGKVMFQGTNAAIEADKWGEAQDKQAKPAKTASSLPKNISQLSVLGSDEVGNGSY
                     FGPLTVCAAYVKRDQLAELRKLGVRDSKELKDSQIIQLADILKKTIPYKLLVLSPEKY
                     NEIQPKYNAVRMKVALHNQAIHLLLKKIEPEKPEAILIDQFTPEANFKKYARLEKNQL
                     QEKLYFVTKGEQYHLAVAAASIISRASFLEELHKASAEAGITLPSGAGTKSDQVAAKL
                     LEKGGMPMLEKYAKLHFANTEKALKLLKK"
     gene            58596..59033
                     /gene="zapA"
                     /locus_tag="U2K71_000172"
     CDS             58596..59033
                     /gene="zapA"
                     /locus_tag="U2K71_000172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HEM7314294.1"
                     /translation="MAHEKTRYKAVIANQTYTIIGRETKHHMDIVTKLINEQLAELKQ
                     LSPQMDNEQAAILMAVNALSDQLKKQERILELEEETAELKKKMIKFTELENRVKRIEA
                     IENEAREVLKENGQADHTIKNHVEAQQILNEKRKGQIKQKSSN"
     gene            59092..59640
                     /locus_tag="U2K71_000173"
     CDS             59092..59640
                     /locus_tag="U2K71_000173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HEM7314295.1"
                     /translation="MLSLLILFLLLIAFFSGARRGFALQIIFAIGYVLSFIAAQHFYK
                     PLASHLELYIPYPAVTPTSKLAFFDQVFAFHLDEAFYAGTAFLIILLIGWLLTRFVGV
                     FVHGLTYVPILRQVDWIAGGILSLIMAYVTIFLILQLLAFVPLDVVQNQFGEHSFARF
                     IVERTPFLTNKMYELWVTNILN"
     gene            59755..62115
                     /locus_tag="U2K71_000174"
     CDS             59755..62115
                     /locus_tag="U2K71_000174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEM7314296.1"
                     /translation="MNNRILETLEFEKVKQMVRQFVVTAQGKEELAELVPVSEEQTIT
                     NWLQETEDGLKVQRLRGGIPIPKLENIRPHMKRIEIGADLNGVELAQVSRVLSTTSEL
                     NRFIDDLYDSEIEFARLYMWADQLVTIPVLSRRLKEAIDEDGRVTDDASPELKSIRQN
                     IRRSEQAVREQLDGIVRGKNAKYLSDAIITMRNDRYVIPVKQEYRGVFGGVVHDQSAS
                     GQTLFIEPKQVVDLNNRLRQYQIAERNEIQRILSELSAELVPHRQEIIHNAYVIGKMD
                     LMNAKARFGKEVKAIVPGISEDNHVVLKQARHPLIDQEKVVSNDITIGKDYQAIVITG
                     PNTGGKTITLKTLGLLQLMGQAGLPIPAGEESQIGIFEEVFADIGDEQSIEQSLSTFS
                     SHMTNTVDILSKVNEKSLVLFDELGAGTDPQEGAALAIAILDDLGKKSAYVMATTHYP
                     ELKVYGYNRANTINASMEFDVDTLSPTYRLLIGVPGRSNAFEISSRLGLDTEVIDEAK
                     QLMNDESQDLNEMITDLENRRKMAETEYLEMRHFVSEAQELHDDLKEAYSYFFEEREK
                     EMEKAKKKANEVVSEAEEKAEKIIADIRKMQQQIGQGNVKEHQLIDAKTQLANLHQEE
                     TLKKNKVLKKAKEQKTLKPGDEVLVTTYGQRGTLLRKNGNQWQVEIGILKMNVSEDEL
                     TPVAPQKEPTQRVIHAVRSESSSHVPNQLDLRGKRYEEALSEVDQYLDSAILAGYPQV
                     TIVHGKGTGALRKGITDYLKNHRSVKSFEFAPANQGGNGATIVKFK"
     gene            62202..62516
                     /gene="trxA"
                     /locus_tag="U2K71_000175"
     CDS             62202..62516
                     /gene="trxA"
                     /locus_tag="U2K71_000175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HEM7314297.1"
                     /translation="MSQVITDKTFNEETDKGLVLIDFWATWCGPCRMQAPILDQLEQE
                     YDEEEFRIAKMDVDENPETPQQFGIMSIPTLMLKKDGQVVEKAVGVHSKEQLRQMIDQ
                     YL"
     gene            62698..64476
                     /gene="uvrC"
                     /locus_tag="U2K71_000176"
     CDS             62698..64476
                     /gene="uvrC"
                     /locus_tag="U2K71_000176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HEM7314298.1"
                     /translation="MNERIKNKLALLPDQPGCYLMKDKNGTIIYVGKAKILKNRVRSY
                     FTGSHNTKTERLVSEIEDFEYIVTESNIEALLLEINLIKKNDPKYNIMLKDDKTYPFL
                     KITNEKYPRLVITRKVLKDKALYFGPYPDVGAANETKKILDRLFPLRKCKPSQTKEPC
                     LYYHLGQCLCPYYFDVDPKVYTKIVDEVKNFLNGGHEKIESEIHQKMEKAAENMEFEK
                     AAEYREQIRAIETIMTRQKMTNTDLLDRDVFGYAIDKGWMCVQVFFVRQGKLIERDVS
                     MFPFYNEAEEDFLTYLGQFYQENEHFIPKEVLIPDTIDKPSVEALLATKVLQPKRGEK
                     KKLVELASKNASVALNERFDLIARKQERTIGAVERLGNAMNIPTPIRIESFDNSNIMG
                     TNPVAAMVVFIDGKPARKEYRKYKIKTVNGPDDYASMREVIYRRYSRVIREGLPLPDL
                     ILIDGGKGQVDVAKDVLENQLGIDIPIAGMAKNDKHKTNELLFGTDLAVVPLERNSPE
                     FFLLQRIQDEVHRFAITFHRQLRSKNSFASRLDEIEGLGPKRKKMLLKEFKSLKNITA
                     ATTEELQEIGLPKNVAQNVFDKLHQE"
     gene            64621..65520
                     /locus_tag="U2K71_000177"
     CDS             64621..65520
                     /locus_tag="U2K71_000177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314299.1"
                     /translation="MLEVKDLVKTFGNYTAVDHVSFQIPDGKIMGLIGQNGAGKTTTF
                     RLILDFLTQDQGEVLWNGHHLGEKDYDVIGYLPEERGLYPKVTIQDQLLYFAELRGKS
                     RKEIEPKIDFWMEKFQVKGKKTDKVKSLSKGNQQKVQLIATLIHEPKLIILDEPFSGL
                     DPVNAELLKDGILELKKHGSCVIFSSHNMDNVEKICDHLIMLRNGKMVLNGKVHEIRE
                     SFGRTKLFLESGLSQQEVEEIEGVKKVILHEDNTLEITLEDPEAGKEIFTRATQFGYI
                     PMFNQQPPTLEEIFKLKAGEIDA"
     gene            65513..66775
                     /locus_tag="U2K71_000178"
     CDS             65513..66775
                     /locus_tag="U2K71_000178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314300.1"
                     /translation="MRKFWIITKDVYLKNVKSISFLIMILVPFIMLGIIYVAGNFAQQ
                     NSDVNKIGVLADNEQISQQMSQVKTDDFEFKAVDSEEKAQKELSEEKIDAYLVVSTEN
                     DKINGMLYSENSLGQSTQLLIQQQLTGLQSMLRASQLGISPEEVASLSQQASLTRQKV
                     NFDSKGKMTVGEDNSTVQYVVSYVATIVLFIIILTYAQIIAQEIASEKGTRIMEVILS
                     STTAQKHFYGKLTGVLLVALTQMALYGIIFGIGYSQFKDMEMVKNILDGISLDNLIGP
                     FLWYSLLFMIFGILIFSVLAALCGSLVNKAEDTAKAILPVTYLSLGGYMLGLILGASD
                     PNNIVIRITSYIPFLSSYIMPVRLANETVGMGGALISLLVLVAVTFLLMILSANMYKS
                     NVLVYSEGGLWSSLKQSISIMRNERKKA"
     gene            66936..68834
                     /locus_tag="U2K71_000179"
     CDS             66936..68834
                     /locus_tag="U2K71_000179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-bisphosphatase class III"
                     /protein_id="HEM7314301.1"
                     /translation="MDRYLDLLSEKFPSTEDVITEIINLEAILQLPKGTELFLSDIHG
                     EFPAFDHILRIGSGNLKEKIKDLFSEQMTETDISQFTIFTAYPEYALTTDWYKEQDKS
                     QLIHHLIDLLRFTTVKYTRSKVRKGLPKEYSYIIEELLYIDDRINGKKDYVKKIIEQL
                     VLMKEEEKFLTKLAQTIQKSVIDHLHIVGDIFDRGTQAAKVMDRIMDFSSVDIQWGNH
                     DILWIGAYCGSEACLLTLLRIAARYNYLFELEKEYGLNLRPLFSFAHQTYQKNPVFAP
                     KNSKNLSEREQEELEKIHQALSILQFKSEHQLLDRRPEFKMDDRKLLEKIDYEASTID
                     LEGQLYELKGTCFQTIQPDDPKKFLEEEQKVIDSLMYSFQHSLRMQKHMTFLIEKGGM
                     YLTNNQHVLFHGCIPVDEKGQLLAFELDQSYRGKELLGFFEKQITESAKDLTAKEDLA
                     TDLIWYAWSGPFSPLFGKSKMATFERYFLTEPHTHVEKSNPYYHLRDEEWFSEKILAE
                     FDTKEEGSAIINGHTPVKVKKGESPIKANGKVFVIDGGLSKAYQKTTGIAGYSLLCNS
                     YGFQIVTHYPFLPIEQQFAKKSDQTNVKKVIEQQLPRKLNRDTTKGMELQQQITQLKR
                     LLDYRKDD"
     gene            complement(69164..69883)
                     /locus_tag="U2K71_000180"
     CDS             complement(69164..69883)
                     /locus_tag="U2K71_000180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyl-gamma-aminobutyrate hydrolase
                     family protein"
                     /protein_id="HEM7314302.1"
                     /translation="MKPIIGIAGNQLIRATDTFQGNQVSYTPQGFVDAVLDAQGLPLI
                     LPVMSPDSAPQLIGQIDKLILAGGQDVSPQLYMEDPHPKLTETNIQRDQFEQALILEA
                     LKQRKPIFAVCRGLQLLNVVLEGTLYQDLSLYPKWSVKHEQQPTAPQFTTHEVKIVSD
                     SLLSNLLPDPYFVNSYHHQAVKDLSPLLKAIAFSNDGLIEAVQSKDDMHKILGVQWHP
                     ELTHRVHPSEQRLFDFFVNEF"
     gene            70220..70552
                     /locus_tag="U2K71_000181"
     CDS             70220..70552
                     /locus_tag="U2K71_000181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314303.1"
                     /translation="MKQVLEALEQIRKAEEENEKRAQTLRQQLKIYATEKEQELETIQ
                     TNYRQQLKQLVEEKEQFEIQELEKEKMKLKAEADKVQATLKDQYQKHQSQAIGAIIER
                     VKETYGRH"
     gene            70539..72542
                     /locus_tag="U2K71_000182"
     CDS             70539..72542
                     /locus_tag="U2K71_000182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit I"
                     /protein_id="HEM7314304.1"
                     /translation="MAVTKMEKVTLISDKRNQERILQAVQGIQNVEIRDLYQNRTNNQ
                     WVEKYFPDTTIIDKEAKLSALGNRLKDIQEAIQFIQHHGDSRQKKLHLKRQELSLQDL
                     EANYSEENFINKLKEVLDLKEKWEQLTEERDRLEEEEEWLLHWQQLDIAPSMYASKTT
                     IFEMGTVSAANFESFKEELSKLDDVYMEEIDYTPKQVYLSYVALKKSVAVIEEIAGKY
                     GFNKETYPYEQNPKEKLQETKQQLSIVHEQQKKITTALGQCSGYIQEFEWVEEVTLAV
                     AEREKIKERFIHATYLIVLQGWVDAEEKQNLLHVLNETVSEEDIYVSFETPNSEEIQQ
                     EVPTKLKNHPLVEPFELLTEMYSLPKYEEVDPTPWMTPFYLVFFGMMVADVGYGLLML
                     IGTILAQKLLVLPRGMKRFVKFFEILSIPSIIWGLIYSSFFGQSLPKELFGIRLPFPI
                     LSTTDDVNTILILSVIFGLIQILVGLFVAAKENIRRKDYLSAVSDGFAWQGILIGIVI
                     ALVGAMLIKNTAFVYLGAGLSIIAALCILIVPIIQTPSKVKGAAKGAYNLYGLTGYIG
                     DLVSYTRLMALGISGGSIGAAFNMLVAFMPPAARFSVGILLIIALHALNMFLTLLSAY
                     VHGARLQYVEFFGKFYTGGGRAFDPLKTAEKYVNINHKKKNNK"
     gene            72561..73031
                     /locus_tag="U2K71_000183"
     CDS             72561..73031
                     /locus_tag="U2K71_000183"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit K"
                     /protein_id="HEM7314305.1"
                     /translation="MMDYLISQNGGMVFAVLAMATATIFSGIGSAKGVGMTGEAAAAL
                     TTSQPEKFGQALILQLLPGTQGLYGFVIAFLIFINLGNDMSVVQGLNFLGASLPIAFT
                     GLFSGIAQGKVAAAGIQILAKKPEHATKGIIFAAMVETYAILGFVISFLLVLNA"
     gene            73052..73636
                     /locus_tag="U2K71_000184"
     CDS             73052..73636
                     /locus_tag="U2K71_000184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase V"
                     /protein_id="HEM7314306.1"
                     /translation="MNAIEKIISQMNEAAEQERAALEQEERMKIDQNFEQKRTQVETE
                     HQKQKEKQIELLEKKYRQLRNRQQVEVRQENLNAKQEFLRRLFADAVTEMENWDESEQ
                     IQFIKNALYSLPLTGKVAFIAGEKSAAYLSQTLLDEWNNELPFMMVLSDETVADQAGF
                     LINDQGVQYNFLFSSLVQDIQGTMSFEIANQLFE"
     gene            73651..74637
                     /locus_tag="U2K71_000185"
     CDS             73651..74637
                     /locus_tag="U2K71_000185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATPase subunit"
                     /protein_id="HEM7314307.1"
                     /translation="MEYHELNPLIRGRELELLSNETFERMIQTNSVEALGEILKSTIY
                     SPYIYDGYEYDFEQNLLKEQSRLFRWLKESAPEPEIVWIYTMRYTFHNLKVLTKAEMT
                     NQNLDHLYIDDGFYSLETLKDAIHTQVSNELPESIMDSIREVRDYFEESTILQGIDVI
                     YDRQFLTEQRRLADKLGYQELLTEVIAFIDLTNITTMARGILQKRTYGFMTTVLSSSG
                     SIEKEVFLNFVDKDMEDYTNFLLETDYADFLRPVIREGNIDLVKLEQLKDDYLSSLYQ
                     EAQTQAFGPLPLLAFLNAKEVESKNLRLLVIGKRSHFESETIRKRVRQVYDA"
     gene            74627..74938
                     /locus_tag="U2K71_000186"
     CDS             74627..74938
                     /locus_tag="U2K71_000186"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit F"
                     /protein_id="HEM7314308.1"
                     /translation="MTHKIGVVGDKDSVLPFKLFGFDVRYGTTKQTVRQAIEEMAKNE
                     YGVIYVTEQCAALSPETIERYKEQMIPAIVLIPNHQGSLGIGLEEIQKNVEKAVGQNI
                     L"
     gene            74960..76741
                     /locus_tag="U2K71_000187"
     CDS             74960..76741
                     /locus_tag="U2K71_000187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit A"
                     /protein_id="HEM7314309.1"
                     /translation="MQIGKIIKVSGPLVMAENMSDASIQDMCLVGDLGVIGEIIEMRG
                     DVASIQVYEETSGIGPGEPVRSTGEALSVELGPGIISQMFDGIQRPLDTFMEITQSNF
                     LGRGVQLPALDHEKKWWFEPTVEAGETVSAGDVIGIVEETKVIKHKIMVPNGIKGTIK
                     TIEKGSFTIDETVCVIETENGDKNLTMLQKWPVRRSRPIKEKLNPDAPMITGQRVIDT
                     FFPVTKGGAAAVPGPFGAGKTVVQHQIAKWADVDMVVYVGCGERGNEMTDVLNEFPEL
                     IDPNTGESLMERTVLIANTSNMPVAAREASIYTGITIAEYFRDMGYDVAIMADSTSRW
                     AEALREMSGRLEEMPGDEGYPAYLGSRLAEYYERSGRVIALGSEGREGSITAISAVSP
                     SGGDISEPVTQNTLRVVKVFWGLDSSLAQKRHFPSIDWIQSYSLYSTEVGKYMDHILQ
                     ADWASMVTEGMRILQEEQQLNEIVRLVGIDSLSDNDRLTLEVAKSIREDYLQQNAFDD
                     VDTFTSREKQFKMLKMILTFGEEGRRALSLGAYLSEIMDGTVSIRERIGRSKYIPETE
                     LEKLDQIIGEIKQTMQTIVSEGGMSND"
     gene            76734..78110
                     /locus_tag="U2K71_000188"
     CDS             76734..78110
                     /locus_tag="U2K71_000188"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit B"
                     /protein_id="HEM7314310.1"
                     /translation="MIKEYRTIGEVVGPLMAVEKVAGVKYEELIEVRMQNGEIRRGQV
                     LEVQEDKAMVQIFEGTSGINLRDSSVRFLGHPLELGVSEDMIGRVFDGLGRPKDNGPE
                     ILPEKHLDINGEVINPVARDYPDEFIQTGISAIDHLNTLVRGQKLPVFSGSGLPHKEL
                     AAQIARQATVLDSSDDFAVVFAAIGITFEEAEFFMEDFRQTGAIDRSVMFMNLANDPA
                     IERIATPRMALTAAEYLAYEKGMHVLVIMTDMTNYAEALREISAARREVPGRRGYPGY
                     LYTNLATLFERAGRIRGLKGSVTQIPILTMPEDDKTHPIPDLTGYITEGQIILTRELY
                     KSGIQPPIDVLPSLSRLKDKGTGPGKTREDHAATMNQLFAAYAQGKQAKELAVVLGES
                     ALSDVDKIYAKFAERFEKEYVNQGFYTNRSITETLDLGWELLSMLPRTELKRIKDDML
                     DKYLPEGK"
     gene            78117..78752
                     /locus_tag="U2K71_000189"
     CDS             78117..78752
                     /locus_tag="U2K71_000189"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="V-type ATP synthase subunit D"
                     /protein_id="HEM7314311.1"
                     /translation="MARLNVNPTRMELTRLKKQLTTATRGHKLLKDKQDELMRQFILL
                     IRKNNQLRQEMEESMTEAMSDFVLANASITEPFIEELFVLPAENVDLSIVDKNIMSVK
                     VPVMNFEYDEQLTDTPLEYGYLNSSAELDRSIDRFTTLLPKLLELTEIEKTCQLMASE
                     IEKTRRRVNALEYMTIPQLEETIYYIRMKLEENERAEVTRLIKVKNMGTTD"
     gene            78782..80137
                     /locus_tag="U2K71_000190"
     CDS             78782..80137
                     /locus_tag="U2K71_000190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trk family potassium uptake protein"
                     /protein_id="HEM7314312.1"
                     /translation="MIKQSKQIKKRLPAVQLLALGFFLLILVGGGLLTLPFFSNSGHG
                     TNFIDALFTATSAVCVTGLTTLNTAEHWNSAGQFLIMLLIEIGGLGFMMVPILCFALI
                     KKKVSLSTRIILKEALNLEEVSGVMKLMLYILKLAFVIQLIGTVALSFVFVPEFGWLK
                     GIWYSLFHAISSFCNAGFDLLGDSLANHQQNVYLIMVVSALIISGGLGFIVWRDLLEY
                     HQKKKMTIHSKIALTVTGTLLLGGFVVFFFTERNGATLVEGNMFQRLANTFFMSVTPR
                     TAGYYSVDYLHMSHAGLILTMFLMYIGGTSGSTAGGLKTTTLGVLLIQMRSMLKGRTR
                     AEAFGRTIRQGAVLRALTLFFVTLTLCVVAIMILSVTETIPKTSGIEYIAFEVFSAFG
                     TVGLTMGLTPDLTAIGKLIIIALMYIGRVGVLTVVFSLMTKAQRTQSKYKYPEESVMI
                     G"
     gene            complement(80329..81723)
                     /locus_tag="U2K71_000191"
     CDS             complement(80329..81723)
                     /locus_tag="U2K71_000191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEM7314313.1"
                     /translation="MPINSYDNYPLTWRPDKQKLKRPYYRSLIQQLEQDILKNKLQKN
                     TQLPSQRELADYLDLNFTTVGKAYKYGIEKGLLYTNIGSGTFISPNVFESITISTNEV
                     TEHIIDFGLVSSFEECNKFVVPFIQSTSNNPNLVGLLNYQDPKGTNFQLTTAANWLKT
                     QGVIPSLESLSIVSGVQNGLAITLSALFSPGDRIATDRYTYANFIELAQLYHLEIVPI
                     DFDEQGMRPDILIQECRKKKIHGVFLMPSCNNPIGFQISHKRREQLAEVIEKEGLWVI
                     EDDIHSFLTTYYQHTIIPPFQNLLPNQTIYLAGMTKFICSGLRVAYLVVPVHVQSKIE
                     QAIFNINVKTSGLDVEVITQILHSNEANRILETKLSLTKRANQLFDSLFKVEKPTNRV
                     PYYRILPVDPEIDPKIIENDFLANGIRLYHSRRFTTRTQPDAFVRISLSSNNLEILAS
                     GLSIVKEKIKKYEL"
     gene            81815..82786
                     /locus_tag="U2K71_000192"
     CDS             81815..82786
                     /locus_tag="U2K71_000192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HEM7314314.1"
                     /translation="MKIINFEEVVASFSFEEAIQVMKKCFFDYESGKISQNPRLVEIL
                     PDGKGKNMFAMMPAYLGENRYFGTKVITAFPDNHQYHLPSHLGEIFLFDAQNGLPKAL
                     INANAVTWIRTAAVSALATDYLANPHAETLALIGAGQQALSHLTAISTIRPITTVFVY
                     DILEERRNTFIAKAKKQYPKITFINAISVEDAVRPAEIICTLTSSKEAFLQKEWVRED
                     AHINAVGTFTPDTREISTSLMETSQIYVDDYSAALTESGDLLIAMAEGDLEESCIVGT
                     LGELVSEKKTFYKGESRPTIFDAVGLAIEDLCCAEYIYNKLEKGALK"
     gene            82783..83565
                     /locus_tag="U2K71_000193"
     CDS             82783..83565
                     /locus_tag="U2K71_000193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HEM7314315.1"
                     /translation="MNLDYYVVDAFADEVFKGNPAAVYVLEEWLPEGTMQKIAIENNL
                     SETAFTVKKNQEFELRWFTPDREIDLCGHATLATAFVLFNYYKIPDETIKFSTQSGNL
                     FVTRIKDYYYMDFPSIMPKKVPILSEYEEAIGAKIKEAYLARDLFFVLEDEKTVAKLQ
                     PDFTAIKNFELGIGVIVTAESVQKDFVSRTFFPKLTINEDPVCGSAHSNLIPYWAERM
                     NKTKLSAYQMSSRGGDLECELKEDRVIIGGKAVLFSKGTSFI"
     gene            complement(83734..84105)
                     /locus_tag="U2K71_000194"
     CDS             complement(83734..84105)
                     /locus_tag="U2K71_000194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM7314316.1"
                     /translation="MKMAHTCVRVKDLEASIEFYQKAFGFEESRRRDFPENKFTLVYL
                     TLPGDDYELELTYNYDHGAYDLGDGYGHIAIAADDLEKLHEQHKAAGLEITDLKGLPG
                     TAPSYYFVIDPDGYKIEVIRG"
     gene            84239..84682
                     /locus_tag="U2K71_000195"
     CDS             84239..84682
                     /locus_tag="U2K71_000195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HEM7314317.1"
                     /translation="MDKPIFGEKKQFADYQTRYAAYIVIERDQQIAVIEAPNGAFFLP
                     GGEIEGEETKEEAIVRELIEEMGIVAEISSYLGEADEYFYSNYRATYYYNPGYFFVAD
                     SWKRIGEPTEKTNKIWWVSPQEALVKLKRGSHRWAVERWIEKNKN"
     gene            complement(84725..85534)
                     /gene="yidA"
                     /locus_tag="U2K71_000196"
     CDS             complement(84725..85534)
                     /gene="yidA"
                     /locus_tag="U2K71_000196"
                     /EC_number="3.1.3.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-phosphatase"
                     /protein_id="HEM7314318.1"
                     /translation="MSIKLVAIDIDGTLLNEKREVTKEVKEAIAAAVAKGVSIVLCTG
                     RPLPGVQEQLNELNLFQDNDYVITYNGALVQQTKSGKIIARHGLTHEDFLEIEVMARR
                     VGSHLHSIDDKAIYTPNRDISAYTIHEASLVKMPLKYRTPEEMTPDMNIVKMMMIDEP
                     EILDAAIARLPQTFRDKYTTVKSAPYYFEVLNKEASKGAAVANLAQHLGIDQDEIMAI
                     GDNENDLSMIEYAGLGVAMGNAVSLVKEAANVITSSNDEHGVAEAIKKYVL"
     gene            complement(85553..86920)
                     /locus_tag="U2K71_000197"
     CDS             complement(85553..86920)
                     /locus_tag="U2K71_000197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM7314319.1"
                     /translation="MTTPYKYQMLPMEKVFRDPVHNYIHVQHQVILDLINSKEVQRLR
                     RIKQLGTSSFTFHGAEHSRFTHSLGVYEISRRICDIFSRNFSKEKIGNGGWDDQERLV
                     TLCAALLHDVGHGPYSHTFEHIFHTNHEAITVEIITSPETEVYQILNRVEEGFPEKVA
                     SVIQKTYPNPQVVQMISSQIDADRMDYLLRDAYFTGTEYGTFDLTRILRVIRPYEGGI
                     AFSMSGMHAVEDYIVSRYQMYVQVYFHPVSRGMEVILEHLLHRAHELYQEDASSFELH
                     SQLLLPFLDGSFTLEEYLKLDDGVLGTYFTQWSESADPILNDLAKRFLNRKPLKSATF
                     SGNRDSKLVQELTLLVEKVGYNPVYYTAVNSSYDLPYDFYRPEQGRHRTQIEILRNDG
                     TLIELSQVSQLVAALAGQEQGDERFFFPKEMVDPSLRDHYDLFDETYQEFASHIRNGA
                     LIEIN"
     gene            complement(86904..87734)
                     /locus_tag="U2K71_000198"
     CDS             complement(86904..87734)
                     /locus_tag="U2K71_000198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoate--protein ligase family protein"
                     /protein_id="HEM7314320.1"
                     /translation="MNPAIRLDQHIYQKNDFSPFALTDILTEYSKEHQQIFLHFWQYD
                     CTVILGMKDVRTPFLSDGIQTLKDAGYAPVIRNSGGLGIVSDEGILNISLIFPQSDTK
                     KITIDDAYEQMLLLTKYAFPKAPIQAFEITHSYCPGTYDLSIHGKKFAGIAQRRIKNG
                     ISVMMYLSVNGNQEKRGQVMKRFYQSALKEDFGTNGYPAVDPATMATLEELLDQPLFV
                     KEVKEAFAKAFEKLYSASDLQDSTDWLKKEIQLEQWNNQIQRMEQRNNIKELHYDNPL
                     "
     gene            87897..88334
                     /locus_tag="U2K71_000199"
     CDS             87897..88334
                     /locus_tag="U2K71_000199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1934 domain-containing protein"
                     /protein_id="HEM7314321.1"
                     /translation="MELSQGTPISIKLRTKVKQQGEVQDFYFDLKGQMVTIGDTLYIR
                     YKEIQEETGDEIPVTIKLVPDGHVQLIRAGEMRMRLKFGYKERLETSYRTPYGMIQIA
                     TFTKELHVSLKDRPTAGKVRIDYDLFMGPEKIGEYYLTLDFTA"
     gene            88410..89030
                     /locus_tag="U2K71_000200"
     CDS             88410..89030
                     /locus_tag="U2K71_000200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit delta"
                     /protein_id="HEM7314322.1"
                     /translation="MEISVFEGANKSELSMIEVAHAILEQRGDVMDFSDLANQIQNYL
                     GKSDSEIRDSLAQFYTDLNIDGSFISLGDNRWGLRSWYAIDSIDEEVNHGMDEEDEDT
                     PRRRKRKKVNAFINDDEDAIDYNDDDPEDTDLTEEDDDDLFDDDDDEDEEIAAYNSDL
                     QEIGADDPTDDEEDLPGNIEEDLSIIDEDDDDEDYEEEEFSDSEKE"
     gene            89219..89725
                     /locus_tag="U2K71_000201"
     CDS             89219..89725
                     /locus_tag="U2K71_000201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HEM7314323.1"
                     /translation="MIFTGYKKKRQIEKYRHLLNGLIETDYQKMFRITMNYVHDKEEA
                     LDVMQDSFHKALNYFEKEKEIEHFSAWFYRILIRTALDSWRRQKRTPVDLFEIQDIPQ
                     QLPLETSVAELHMILDKIDSPEKEILILYFFEGFRLKEIATILDMNENTVKTKMYRSL
                     NALRQILE"
     gene            89742..90641
                     /locus_tag="U2K71_000202"
     CDS             89742..90641
                     /locus_tag="U2K71_000202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3298 domain-containing protein"
                     /protein_id="HEM7314324.1"
                     /translation="MYSQKENLKKLKKTYKEQAIPKGLKEEIRQRFIIEEQSFIKAKR
                     RKRRLQGFGVSLAIVMITTSSLLFNSQVRSFAEDLPILGSVIELILGERFTDRSEKID
                     IQVPKINTQNEKENKTIHGLNQKYFREGQADFEKAEKEYGNFETDHYQVLGDYQKIVD
                     DNRFLVIERQITQTAADSHVEKRYDTIDKKNSVQLSLPLLFKDDTYLSVLTKEVKRQM
                     AEQVKEDPSKYYWTEQDIQEGTIEKPTLVTPTRSFYLNKEHQLVLTFSQYEIAPGYMG
                     TPEFVIPKSVTKTILASEDYLDH"
     gene            90728..91612
                     /locus_tag="U2K71_000203"
     CDS             90728..91612
                     /locus_tag="U2K71_000203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEM7314325.1"
                     /translation="MKRNYLVISIIALSVLLFFEITSVASLITEVKGQTHSSEHSSQS
                     FKQSEQSTIKSQSTETANVKEAIQESAEKVQSQSSQSTQDSTKTSRDPAVKSVAISFD
                     DGPGATTTPQLLRILKEKNVHATFFVLGENTAQHPEIVKQTAEAGHEIGNHTYDHQNL
                     ATLSAQSMTEEVTKADTEIKKVTGKTPTFVRPPYGSVTNVGATIIQRPIIEWSVDSED
                     WKTRNPDLILQKIQATVYDGAIILFHDIYPETIRAVPQVIDYLKEQGYRITTVGDLLG
                     HPTAVENYYGRNDHRPVQ"
     gene            complement(91804..91875)
                     /locus_tag="U2K71_000204"
     tRNA            complement(91804..91875)
                     /locus_tag="U2K71_000204"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(91840..91842),aa:Arg,seq:ccg)
     gene            91935..93269
                     /gene="rpoN"
                     /locus_tag="U2K71_000205"
     CDS             91935..93269
                     /gene="rpoN"
                     /locus_tag="U2K71_000205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HEM7314326.1"
                     /translation="MKFEQNFSQKQQQTQKLAMTQQLQQSIQVLQYNSEELLAFVENQ
                     AMENPLVEVVEPEWQPDYIKASSSSYEGEETNYLNQIPDTKGSLFDSLIEQVHLNYRD
                     TFLRKIVLYLVEYIDLNGFLTISLEEAGKEIGATPIQMLDALTLIQQLEPAGVGARSL
                     QECLMLQTERDDYAPELAYIVLEECFEELVERKWKEIAQRFSVDLHAVQQIFDYLQTL
                     SPSPGRIFDRSSELYIIPDVRVLVDDDKNVQVISNRKNQPNIRFQESYFKQMSQQADK
                     ETENYLKERKQEFEWLKKTILQRGDTILRVAQVIVSRQKAFFIDKERPIKPLTLKEVA
                     TEIDVHESTVSRAVNGKYLETDFGVFELKKFFTTRIPTNSTEQTEDLSADTAKKKLQE
                     LVDQEDKNKPLSDQKLVELLKKDEIAISRRTVAKYRDLLGIPSSSKRKRYDN"
     gene            93418..94509
                     /locus_tag="U2K71_000206"
     CDS             93418..94509
                     /locus_tag="U2K71_000206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="central glycolytic genes regulator"
                     /protein_id="HEM7314327.1"
                     /translation="MGRFKSTLDVKCPTKEIAMLDEIKMIESVAPDIIEVLQERYKIL
                     RNIYWMQPIGRRSLSESMGITERVLRTETDLLKNLNLIDTSKSGMTLTTKGEEVYQSL
                     ENFMDQLLGTHQTEQQLAEYFGNQRCIVVSGNSEEQTKVADAFGEALSEALDRLLPEG
                     ENIIAVMGGTTMAVVAEQLSNLENKKRHNLFVPARGGIGEAITVQANSVSARMAAKAN
                     GNHRALYVPEQLSLATYNSLLNEPSIQEVLNLISEANCVIHSIGRALHMAARRKMTEK
                     ELVMLKQANAVAESFGYFFNEKGEVVYKVPRIGIELQDLEKVPIIMAIAGGKSKAKAI
                     RAYMKNAPKQTWLITDEAAANEILKGVTL"
     gene            94550..95551
                     /gene="gap"
                     /locus_tag="U2K71_000207"
     CDS             94550..95551
                     /gene="gap"
                     /locus_tag="U2K71_000207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEM7314328.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIQDVDGIEVVAINDLTDAKMLAHL
                     LKYDTTQGRFNGTVEVHEGSFNVNGKEVKVLANRNPEELPWGELGVDIVLECTGFFTS
                     KSAAEKHLTAGAKRVVISAPGGNDVPTIVYNTNHETLTGKETVISGASCTTNCLAPMA
                     KTLNDKFGVVEGLMTTIHAYTGDQMTLDGPHPKGDFRRARAAAANIVPNSTGAAKAIG
                     LVIPELNGKLDGAAQRVPVPTGSLTELVTVLEKEVTVDEINAAMKEASNESYGYNTDE
                     IVSSDIVGMTYGSLFDATQTKVMTVGDKQLVKTVAWYDNEMSYTAQLVRTLEYFANL"
     gene            95661..96851
                     /locus_tag="U2K71_000208"
     CDS             95661..96851
                     /locus_tag="U2K71_000208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005228679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HEM7314329.1"
                     /translation="MAKKTVKDIDLKDKKVLVRVDFNVPLKDGVITDDTRIKAALPTI
                     NYVLEQGGKAILFSHLGRVKTEEDKEGKSLAPVAKRLGELLGKEVTFVPETRGEQLEE
                     AIRNMNDGDVVVFENTRFEDIDGKKESKNDTELGKYWASLGDVFVNDAFGTAHRAHAS
                     NVGIASTGIPTVAGFLMEKEIKFIGEAVEEPKRPMIAILGGAKVSDKIGVIENLIPKA
                     DKILIGGGMTYTFYEAKGIKIGNSLVEADKVELAKELIEKAGDKLVLPIDNVCAPEFS
                     NDVETQVIEGDIPDGLMALDIGPASVKLFADTLQGAKTVVWNGPMGVFEMSNFAKGTI
                     GVCEAIANLEGATTIIGGGDSAAAAEQLGFADKFTHISTGGGASLELLEGKELPGLAA
                     INDK"
     gene            96955..97710
                     /locus_tag="U2K71_000209"
     CDS             96955..97710
                     /locus_tag="U2K71_000209"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HEM7314330.1"
                     /translation="MRKPIIAGNWKMNKTASEAKEFAEAVKNKIPSNDKVDSVIGSPA
                     LFLQELMTSAEGTELKISAQNCYWENSGAFTGETSPAALADLGVHYVIIGHSERREYF
                     HETDEDINKKAKAIFANGMTPILCCGESLETYEAGKTAEWIEGQITAGLADLSAEQVS
                     NMVIAYEPIWAIGTGKSADAQIADEICGVVRQTVEKLYGKEVSEAVRIQYGGSVKPEN
                     IAEYMAKENVDGALVGGASLQADSFLSLLDAVK"
     gene            97826..99124
                     /gene="eno"
                     /locus_tag="U2K71_000210"
     CDS             97826..99124
                     /gene="eno"
                     /locus_tag="U2K71_000210"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HEM7314331.1"
                     /translation="MSIITDVYAREILDSRGNPTIEVEVYTESGAFGRGMVPSGASTG
                     EYEAVELRDGDKARYGGKGVTKAVDNVNNIIAEAIIGYDVRDQMAIDKAMIALDGTPN
                     KGKLGANAILGVSIAVARAAADYLEVPLYHYLGGFNTKVLPTPMMNIINGGSHADNSI
                     DFQEFMIMPVGAPTFKEALRMGAEVFHALASILKARGLATSVGDEGGFAPNLGSNEEG
                     FEVIIEAIEKAGYVPGKDVVLAMDAASSEFYDKEKGVYVLADSGEGEKTTDEMIKFYE
                     ELVSKYPIISIEDGLDENDWDGFKKLTDVLGDKVQLVGDDLFVTNTQKLSEGIEKGIA
                     NSILIKVNQIGTLTETFEAIEMAKEAGYTAVVSHRSGETEDSTISDIAVATNAGQIKT
                     GSLSRTDRIAKYNQLLRIEDQLGEVAEYKGLKSFYNLKNK"
     gene            99523..100662
                     /locus_tag="U2K71_000211"
     CDS             99523..100662
                     /locus_tag="U2K71_000211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgE/DapE family deacylase"
                     /protein_id="HEM7314332.1"
                     /translation="MKKEEKIAILQEIIRIKSVNGNEGEVAAYLNKLLAKHDITGEIV
                     SYRDGRDNLIARYQKGQSGKVLGLSGHMDVVAAGDESSWTYAPFAAEIHGNRLYGRGA
                     TDMKSGLAAMVIAMIELKESGKPFNGTVKLLATVGEEVGELGGEQLTKAGYVDDLDAL
                     IIGEPTNYSLMYTHMGSINYTVTSHGKEAHSSMPDQGYNAINHLNEFITKANAEMNHL
                     AETIENPVLGKTIHNVTLISGGNQVNSIPSHAQLQGNIRSIPEYPNDKIIALLQSIVN
                     ELNQETDYHLELMIDYNKIPVKADPDSPLIHCIQQQFSQPLPLVGAAATTDAAEFTKA
                     NHSFDFVVFGPGVVTLPHQVDEYVEIDNYLDMIEKYQGIILSYLA"
BASE COUNT        33405 a        17203 c        20783 g        29316 t
ORIGIN      
        1 ttaaataaat aggaacaaaa aaattaaatt atttaataca cacattaggg atcattcatt
       61 gaaggattag aagacaacga ggatggcgaa aaatatgatt acaatagaaa agtcgttcta
      121 aaccaatcag ttcctagtat cacacaacgc aagccagtcg atttggtgag attagaggag
      181 tgaaagcaaa ctttattata taagaaaaaa tattcgaaag ggatgctagt ttaggtacta
      241 gtgctgatag attaatatga tgcctcgtaa tattaggcgt agttactcta gagacatcac
      301 tatggactat gatttgtctc agagtcacaa catgtttttt tatgaaaaga aacagctaaa
      361 ataattaaat tgcgcaatgt ttaaactttc ttaaactata acctaataaa tgtgaagaaa
      421 taatgcacaa tttcttcaca tttatcttct attctatata cataccaacc aacaacccta
      481 tatacttttt catttttact cctcaagtaa aaataactcc tagaccgcgg ctacccaccg
      541 cggtcgtttt tttattcttc aatcaaaatc gctttgatac ccaaacctgc caagattcgg
      601 gcagaagtat aagtattcaa tcctcttctg aaagtaatgt aaactttttt atcttttggg
      661 atttcattga ttcgatctcg taactcattc attggaatat tttttgttgc cgagatactg
      721 ccagaaggtg ctttattagg ttcacgtata tctaaaaaga acgcttcttt caaatcgttt
      781 tcgggtatat cacttgcttt gatcgtttct acgatattcg tcatttgatt gattgcgaca
      841 taaccagcta tattcaacgg gtcacgggta gtagaatacg gtggagagta aggcaactcc
      901 aaatcaggca aatcaaagac tgtcaagttt ccggtgattg ctacagaaag ttctcccatc
      961 cgtttatcga cacctttttc tccaaatgct tgtccaccta aaatacgacc gctatctgca
     1021 tcaaaaatca gctttagatt taatcttgta gcttctggat agaaataggc atggtcaaag
     1081 ggagtgataa aaacagtttt ataatttgtt atgcctgatg cttttaattc atgttctgtc
     1141 attcccgcat aacttgctgt atgatcaaag atttttgcga cacctgcacc aatatatccg
     1201 cgatagcgca taggcgtacc atttaaaata tctgccagca tatgtccttg acgattggcc
     1261 gcactggaaa gcatactttg gatcggctga ccagtcacta cactagtcgt ttcgataata
     1321 tcgccaatcg catagatgtc cggcagattg gtttgtaact ggtcatcaac aatgatctga
     1381 cctgtatctg ataattgtat gccggttgct ttcaccactt cattcttcgg agaaacacca
     1441 acagcaaaaa ttagcatatc agcagaaagg acggagccat ctgataatac tatttctcga
     1501 cctttatcac ggatctcagt aactcttgtt tctaaatgaa cggctaatcc ttctttcagc
     1561 aatttgtcat aaactaaatt agcaatttct ggatcataag ggaaagcgac ttgtggcaat
     1621 tgatcgatta aagtcacgtc taagccgcga tgcttgaaat tctcagctaa ttccagaccc
     1681 ataacacctg caccaagaat gacgacatgt tgtggatttt tttcttcaag gaacgatttg
     1741 attcgatccg catccgtcac actacggagg acaaagccgt tttctgcttc aaaaactccg
     1801 ggaatatccg gataatctgg tctagcacca gaagagatga tcaacttatc gtaatctgtt
     1861 tttatttctt catttgtgga taggtcttta actgttaatc gcttagtaga aggatcgatt
     1921 gctgttactt cgtgtttagt aaagacatcg atgttgtttt ttgttttcaa tatttctggt
     1981 gtacgttcga tcagcgagtc acggtcagtg atcacacctc ctaaataata aggcaaagca
     2041 caacttgcat aagaaatatt ttctccgcgt tcatagataa tgatttcgtg cgcttcattc
     2101 aatcgacgga aacgagtggc aaatgaagga ccgccagcta caccgccaat gacaacaact
     2161 ttcataaaaa catctccttt tctacgttga tcgtactatg ttatggtaaa aaacagcaaa
     2221 taattgtgcc ggaaaattat tgtttatttt gaatagacag aaaatataga gaaataattt
     2281 gtggataaaa cctaaaaatt tttttctatt ttaaggatag ataatacttt gaaggggata
     2341 accctgtata ttttttaaaa gctgaagaaa aatgatggat cgaatgaaat cctaatatat
     2401 ctgcaatttc tgtataggtt aattcatttt ctagcacgag acgttttgct ttttccattt
     2461 ttagttcttt aaaatagcta ataacacttt ttccattcac ttttctaaaa tgttttttta
     2521 agctggctgg actgataaaa aaaatttggg ctaattgttc aagcaaaaaa ttttctgaaa
     2581 gatgttcttc taaatatcgt atgattggat caactaattc atctgaataa agaatatgct
     2641 tttttttagg aatgactttc tcatttgaag gtattgtcaa taaagcaagt tcctgttgat
     2701 aatgccttgg taaggaagac aattgaattt tttgcagaca ataagtaact aataaggaaa
     2761 gcaaatgaat gctcatctgt tggcatagta tgaaaaaaga attgatttcc tgatcatagg
     2821 aaatagctgg cttactatgt tttagataat caattttgaa ctgttcattt ttcagaaaag
     2881 taatgataga tgttatttct tgatctatag aaaaagattg tcccgtaata tgtgctaata
     2941 attgctcatc aaaataaaac tggataatga taaaagtttg tgtttccttt tgagtagaaa
     3001 agatttggac ttcttgctgt ttatcaatga ataaaaggga aggattatct agttctatag
     3061 atttatgtgc aaattcagca tataaattag cgctttcatt tattagtagt cgaaaatagg
     3121 gagcagcaga taaatgaaga tgggtatcta tggaataggt tagtgttttt ttgacaagat
     3181 tggaggattg gttgtttgtt aacaaaagaa ccactttcct ttctcttttt atataaaaag
     3241 ctttctattt ataattaatg actattataa caaaaatgaa aagccaaaac gacaagtaaa
     3301 atattttttt atataaatat ttgtgttact aattccgtta atatacataa gtgaaagcgt
     3361 tttatataag aacttgaaaa gaaagaaggg atagacatga aaaaaagata caaattggtg
     3421 atagctttgc taaacattac cctattattc agcttaagtg catgtggaaa aactaacgaa
     3481 gaagtaacag caaacatcgc agatccaatg ataaaaaaag ccactacatt agaagtatat
     3541 gtcgcaaacg gcaaactgac aaactatgtt gaaaatgctg caaaaatcta taaccgcaaa
     3601 actggagcaa atattgactt aaaaattgtg aatgtagctt cgggaacagc aacagtacaa
     3661 atgatcaccc caaaattggt aacacacgaa gatatgccgg atattatctc catggcagac
     3721 acttcagcag caggtgtgct ggaaaaattt caagatgctt tttatagtgc tgatgaatat
     3781 ggattttatg acaaatatgg cgatcagttt tatgatcaaa aattgaatat tttgcgtcaa
     3841 caaacgagta atgaaacgat tattccttgg gctagtgatt ttaccccagc actttgttat
     3901 tatcaaccaa aaaaatttgc tgaaataggt gtagattttg aagaaatcag ttcttgggaa
     3961 gaatatatcc aggtggcaaa agcattaaaa gaaaaaactg gtagttttgg aattgccttg
     4021 ccagaatctg gggaccaaga attgtttatc aacatgctag cccagcagaa tcaatctttg
     4081 cttgatgaag aaggaaatat ccgtctgaat actaaagaag cgaaacatgc tgcaaagtta
     4141 atcaaagaaa tgatcgactc agatattgta catttctacg gtgcacaaga tgctgagaaa
     4201 gcatttcaag aatctgcaat gtttgttgct ggcggctggt atgctacgaa tatgtctttg
     4261 aattttcctg atgcatctga caaatggcgc atggctccac tgattccttt ttcaaaagaa
     4321 aatccaggaa aaggtgcagt atcaggagga tcaagttttt atgtccctaa agatgcaaaa
     4381 aatccacaag tggcacaaca atttcttaca tttatgctga cagatgaaga atgcttagcc
     4441 aacgcactgg agctgggcgt ggctacatct aactcattgg cctatcaaac agaagcagct
     4501 gagaaaaagt ttgactacta aggaggacaa aaatactatg aactattgaa taaaatcaat
     4561 gaaaatacat caaatactgt tttaccagcc acttattcag atgctacgtc ttatttgact
     4621 accgctgcat ataactattg gcaaacaggt gattttgaaa attcatatct aaaagaagca
     4681 gaaaattttg ctcataagta cggcgtaaaa ttaactggtg attaaaaaat attaggagga
     4741 cttaacgatg atgcaatctt cgttaccaag caagacaata aagaacagca agaaaaaagc
     4801 aacagatacg tctcataaaa aaagtgctta tggattggtc acaccttttg ttattttctt
     4861 tattgtgacg attttgattc ccatcattat ctcgtttgct tttagtttta tgaaaattgg
     4921 ctactcattt gaatttgttg gatttgacaa ttatctagct atcttccgag atccattgtt
     4981 tattaaatct tacacgaatg ttttgattat catggcagga tcaattccaa taacgatgct
     5041 attagctatt tactttgctg ttttattgaa ttcagcacag ttaaaaggaa aaggattttt
     5101 tagaactgtc tattatattc caaccattac atcaacggtg gctgtagctt ccgtattcat
     5161 gacttttttc aatccgactg ggttattcaa tggatttttg aatgcgctag gaattaacgg
     5221 tgtacagtgg ttaacaaatc ctttttggat acgaatatca atgatcatca cgatgatttg
     5281 gcttagtgta ggatataaca ctattttatt tttagctggt cttcaaggga tttctaaaga
     5341 gatatacgaa agtgctgaaa tagatggagc atcgaaattt cggcaattct ttttattaac
     5401 gatccccatg ctaaagccaa tcattttaat ggccacagtt ttagccacta tcaatggctt
     5461 gggtaatttc aatatcccaa atatattctt tgggacttcc aatggaccag aaaattcagc
     5521 tttggtcgta ggagtcaatt tatataaaac aagttttgaa atggttgatt ttggaaaagc
     5581 ctctgctatt gcctggacga tggtgtttgt atctgttata ttatcggcta ttcaatttaa
     5641 attcggaggt gataaaaatg actaaacaaa agatacaaag cagaattttt acgtacgtca
     5701 ttattgtgtt tggtgcctta atatttttga tgccatattt atatatgatt gtagcttcaa
     5761 cgcaaagtaa tcaagaaatt ttaagtggcg gaatcaattt caagtttggg gatatgctat
     5821 taaaaaactg gcaagccatc atggcgagat ataattatcc cttgattatt tggaattcga
     5881 ttgttattgc tgtaatcagt acagttttag ctacagccag ttctacaatg gcgggttatg
     5941 cattagcaaa gtatcgtttt atggggaata aactgttgtt caatacagtt atgctatcta
     6001 ggatggtgcc tttttttacc acattgattc ctttatttta cattatgagt cgtttaggat
     6061 tatcgaatac ttatgcaggt gtgatcatcc cttcgattgc cagtacaact tcagtgttta
     6121 tgatgcgaca atatagcagt caatttccta cagcactgat ggaggctgcc agaatcgatg
     6181 gtgccaatga gtgggtgatt tttacgaaaa tcgctgtacc gattttatca cctacgatta
     6241 ttactagtgg tttacttatc tttatgggaa gttggaatca atatctattt ccattagtca
     6301 tgctttcaga taaagataaa tatacggttc ccttaattat caaaaatatg acggtttcta
     6361 gtacaggtga gccaattaat tatggcgcaa ttatgctaat tttagcaact tcagtgatcc
     6421 caatggtctt gatttatgtg tgggcgcagt ctaaatttaa ggagaatgac attggagctg
     6481 caaataagta aaaaaaataa cgaatgttaa ccatctagtt cgttctagga gcaactccac
     6541 attcataaaa attaaaagaa tggcgggata tccatgaatg aacagacgaa ggaatggtca
     6601 agaaaaattt tgaatgtagc ttctgaaaag atagatgcgg aattagacag aatggggaat
     6661 aaaattccat atatttctta caacggaaaa tatacagacg taataaagga cagaggaatc
     6721 gactggtgga ccaatggttt ttggagtggt attttatggc agctctttca caagacaaaa
     6781 cgagaaaagt tcaaagaggc agcaatacaa caagaacaat tattagaatc tgccctgtac
     6841 catttttcag acgttcatca tgatgtaggt tttttattct taccatcggc tattgcacat
     6901 tatcaacagt ctcaaaacaa acatagtcga caactagggt tacatgcagc tacggtactt
     6961 gctggacgct ataatatttc agggaactat ttagtagcat ggaatgacca taaacctggt
     7021 tggatgatta ttgatagtat gatgaattta ccattactgt attgggcttc gagggaaact
     7081 ggtgatccga ggttctctaa aattgctcgg cagcatgcag atacaacagc taagtatcta
     7141 gtgaggaaag atggatcaac cgggcatatc gcagaatttg atgcagaatc agggaaatat
     7201 attcggttac ttggcgggca aggatacaat gagaattctt gttggagcag aggaaatgca
     7261 tgggctttat atggattcgc gttaagttat cgttacacaa aaaatccgta ctatctagct
     7321 atagcagaaa agatagcagt aaattttatt cagaaaatcg aaaaaactgg ttatattcct
     7381 ctcattgatt ttgctgtaaa agattcgaat attgatacca gtgctggagt atgcgctgca
     7441 tgcggtcttc tggaattagc aggatatgtt tcctctgtac gatcaaaaat gtatcatgaa
     7501 gctgctatca atatactcca aaagacagaa aagcactatg caaattggaa tatggatgaa
     7561 gatgggatca ttggaggcgg agcagaagca taccatagag aactgactta taatgtgcca
     7621 ctgatctatt ctgattattt ttttattgaa ggcttacttc gattagaaca gtcggaatta
     7681 gctatttggt agtaaaaaga ggagaaaata caatgaaaca tccagtacca ctattaagga
     7741 ccagacagga ttactttgac ttatttaaaa agataaatca accactccag ccgttttatc
     7801 gagcaagtgg cgcacaaatt gattatatac atcaaggggt aggatatgga aataaaattg
     7861 caggaatgga aggattctct cgggttttat ggggagcggg gcccgcaatt gaccaattag
     7921 atgaacgatg gttgaaatta attctatcag gaataaaagc tggaacagat ccttcccatc
     7981 ccgattattg gggagaaata catgaaagag accagcgtat ggtagaaatg cccgcaatgt
     8041 tattagcgtt atatcatcat gaacaattat tatggaaaaa attagataca ggagaaaaaa
     8101 gccagattat tcattggttc tcgcaaattt ttgaatatga atgtgcagat ggtaattggc
     8161 aattttttaa gataattgtc ggaacaatta tccaaaaatt tggttatcca gttaaagaat
     8221 cagcactgaa agaagcattt gataatatag aaaattgtta tcttcaaaat ggctggtatc
     8281 gagacagttc aagaggtcgc caagattatt ataatccttt tgcatttcat tattatggtt
     8341 tgatttatag cgtactagaa ccagaaaagc ccattagcca gatatataag aaccgagcta
     8401 ttgatttttc tagagactat attcattttt ttgcggaaga tggtgcaaat gtgccttttg
     8461 gtcgaagcat gatttatcgt tttgcagttt caagcttttg gtcagctatg ttgtgggccg
     8521 atcttttgcc ttttaaatta ggtgaaatca aggggttagt caatcggaat ataagatggt
     8581 ggatgcaaaa agaaatattt gatgaaaaag gtatcctgaa tattggttat acctaccctc
     8641 aattgacatt gactgaaccc tataatagta cgttatctcc attatggctg aataagattt
     8701 ttttattgtt ggcgctacct gaagaccatg agtattggac ggctaaagaa atgcctatgc
     8761 ctattcgtga aggaactaaa cttctttcag aagccaattt gctcatacaa catgataatg
     8821 gacatacatt ttttttgaat gcgggacaat tagggccaaa tttccataca ttgaccaatg
     8881 aaaaatattt aaaatttgct tattctagtc aatttggttt ttcgataccg cgagcgaatc
     8941 aattgaaagc agaaatggca atggattcaa tgctagggat tcagcgagcg gacacatata
     9001 tcacgaccag ttttaagaat gagagcatca aaacctatgg acagttcatc gttcgaaata
     9061 atgtacgaga cattgtatgt aagaaagagg tacttgcctc tacatgggaa cctactttac
     9121 aggcaaaaat cagaacttgg cttattcctt ttgaaggctg gcagattcgc gtacacaaag
     9181 tagaattaga tacagaatat gttctatatg agacagggtt tgctattgaa tgttctcctg
     9241 agaaagaagg aataatcata gaagaagaca aggaaaacta tagggtgagt gaagctggct
     9301 tttcaggtat tatctgttta agtgacgata ctattaaacg aaaatccaca gctattatgg
     9361 gtttgcccaa tacaaacttg atgacatggg aaaacacttt tattcctgga ttagagggta
     9421 catttggaaa aggaacccat tggctcggaa caggagtggt tgctcatcaa gatcgggatt
     9481 atagtttaga agtatggaac aatcgtccca aaattgattt tgatgggaca accgggctaa
     9541 ctatccaaaa taaaaacaat aaaaaaagac taaaattatg ctaaaggctg gtggggaaat
     9601 gcttgataaa gtgatgtttg aagagataat acatccagaa aaatggataa atgaatgtta
     9661 taaagaatta atggaaaaag aatggaataa tgagatcgag cgttgtcgca gacacacgga
     9721 gaggttaatc aaacgtatca gtaacagtca gtatcaattc ccaagcgagt gtcctataaa
     9781 aggacgttat ccattaagag acaatattgg ttggacagaa ggattttgga caggaattaa
     9841 ttggttgtta tttgagaaga cgggtgatcc tttatttcaa caaacagctg aaaaagcaga
     9901 tatagatttt atccacagga ttgactgtca aattatcact aatcatcacg atcttggttt
     9961 tttgtattct ttatcaaccg tagcagatta taaaataacg ggaaatcgaa aaatgaagga
    10021 agcttcaatt gatgctgcag aaacattact tcagcgtttt tgggagaagg caggagttat
    10081 acaagcttgg ggagatccta gtgatccttc acaacaaggt agaatgatca tggattgttg
    10141 catgaatctt ccattattgt attgggcgag tgaagaaact ggaaatcaaa aatataaaaa
    10201 agctgctcag gctcatattc aaaatgcagc agattattta gtaagagaag atggttcaac
    10261 ttatcataca ttttatatgg atgtcaaaga tggaaaacca agatatggaa gtacgcatca
    10321 aggttatagt gataattcct gttgggctag aggccaagca tgggggattt acggttttgc
    10381 attaagttat cgttatttga aagaacaaaa atattttgat ttaagcaaaa tattagccaa
    10441 ttattttatg aatcggttgc cagaagatac catttgttat tgggatttga tctttgatga
    10501 ttctagcaga gaagaaaaag atacatctag tatggtgatc atggtttgtg gattattaga
    10561 attaatagaa tcaggtctat tagacaaaac agaaaagaac ttataccagt ctgctgtttg
    10621 gaaaattatg cgacaattga aagaaagata tgctacaacg gaagaagaag atggcttttt
    10681 aacacagtct gtttataata agcccaaaaa taatggggta aatacacctt gtatttgggg
    10741 tgattactat tatttagaag ctttagtgag aatgactagt aattggtctt cttattggtg
    10801 aaaaaggtaa atatggaatt tttgtttgat aaaataaaaa aagaaaaacc aaaacagcat
    10861 gaatggtttc tgttttggtt ttttcgttaa ttttcatatt cttctaattc caagcttagt
    10921 tcttcccatt ccgtcaattt tgtttcttga tcttgtctag cagcttctaa atcttggttc
    10981 agctcattga gttttacgtg atcttccagg ttttctggcg cgaccatttc ttgttccaac
    11041 tgatcgatcc attcatcgat ccgcgccatt tcttcttcaa cctgagtaat ttttcgttga
    11101 aggctacgga taagcttttg ctgttctttg ctttggtaat aatcagattt gttcgtggga
    11161 gcaggagcag attctggctc atcttgtgcc aacaattcgg cgatttcttc ttcttcctgc
    11221 tttttctcta aataataatc atagtctcct agatagactt tgctgccatt ttcagagagt
    11281 tcgacgactt ttgtcgcgat ccgattgatg aaatatcggt catgggaaac aaaaagcagt
    11341 gtgccgtcat aatcgatcaa tgcattttct aatacttctt tgttgtcaat atctaaatgg
    11401 tttgttggtt catccaaaat caaaaaattt tctttgttca tcgctaactt ggctaaagca
    11461 acccgtgctt tttcaccgcc acttagcaga gggattggtt tttctacatc ttcgccagaa
    11521 aataagaaag cacctaaagc acttcggatc tctttttctg gagtggtagg atgctcatcc
    11581 cacaattctg ctaggatcgt cttgtttgta tgcagatcag cttgaccttg atcgtaatag
    11641 ccgatggtga catttgtccc tagcgtttct tttccacgga taaaagggat ctggtgaatg
    11701 attgatttca gcaacgtcga tttcccgata ccattcggac ctactagagc aatcgcttct
    11761 tgacgacgga tatctagatt gattggttca gataaggtga cattatcata tccgatagcg
    11821 gcatcttcga tttgcaagac tacatttcca gaaggctttt ctatactgaa tagaaagttt
    11881 gcttgttttt catccccttg aggacgatcc agccggtcca ttttttctaa tgtctttctg
    11941 cggctttgcg ctcgttttgt ggtagaggcg cgaacaaggt tccgagcaac gaagtcttct
    12001 aatttatgga tttctgtttg ctgcttttca taagccttcc attcacttgc taactgttct
    12061 gctttcaaat ctagatattt cgaatagtta cctttgtaat ggcgcatttt tcttctgctt
    12121 aattcataga cttcattgac tactttatct aaaaaataac ggtcatggga aacaatcaat
    12181 aatgcaccag tatagctttg cagataattc tcaagccagg aaagtgtctc gatatctaaa
    12241 tggttcgttg gttcatctag aatcaaaatg tcagggtgtt gcaaaagcat gcgagctaaa
    12301 gctaaacgtg tgcgctgtcc gcctgataaa gtatcgattt tctgatcata aaatgaagaa
    12361 tcgaatttaa acccatgcaa tacggaacga atttcatttt cataaccata gccgtttcga
    12421 tctgaaaaat cgtgttgcag ccggtcgtat tcttttaaat aagaactata agcaggtgtg
    12481 tctggcgtac cttcgctaat gaaaatctct agctcgcgca tccgctcttc catttgtcta
    12541 acttgttcaa aagctttcag catctcgttc caaactgttt cattggaatc aagtcctgtg
    12601 tcctgagcga ggtatccgag agtcgctgtt ttatttttag cgatctttcc ttcatcaggt
    12661 gcttcaattc cagcaatgat ctttaataaa gtcgattttc cagcgccatt tctgccaact
    12721 aaagcgacac gagcgcctgt actgatttct aaatgtatgt tttcaaataa gacttctgca
    12781 ccaaaatggc gagcaatctg attggcttgt aataatatca tggaatcctc ttttctactt
    12841 tgtgaattct cctcaaataa aaaactattt ttcaattagt agtctaccat aaattccatt
    12901 atactgcgag atttgcagaa atgataaata aatgaataaa gaataaaatt tcacaatatc
    12961 tgagcaatgg aattgatttt ttcctataaa tactgctatg atggagaaat caaagatgta
    13021 attagacaat acgtggaggt catttcgtga aagatcatat tattccaaag gcgacagcta
    13081 aaagactgcc tttgtattat cgctatttaa ggattttaaa taatgctgga aagaaaaaag
    13141 tgtcttcaac tgaattaagt gaagccgttc aagtagatag tgcaacgatc cgtcgagatt
    13201 tttcttattt tggcgaatta ggaaaacgtg ggtatggtta tgatgtagaa gatcttatgc
    13261 acttttttgc aaagacatta aacgacgatc aattgacaaa tgttgcgctg atcggtgtag
    13321 ggaacttggg aagtgcttta ctaaaataca aattccatca aagtaatagc atacgtatca
    13381 gttgtgcttt tgatgtgaat gaggaaatcg ttgggcgcat cgtagacggt atccccgttt
    13441 atccaatgga agatatgtca gaacaaatca agatccaaca aatcgaagta gcaattttaa
    13501 cgatacccgc tgaaagagca caagaagtag tcaatcagtt aacggaagca ggaatcaaag
    13561 gaatcttgaa ctttaccgct gctcgattga atgctccgtc agatgtattg atccaaaatg
    13621 ttgatttgac gaatgaattg caaaccttga tctatttctt gcattctgac gcaaatgcgc
    13681 attccgaaaa tacagcagaa taaaaataaa aagcgattga acaaatgaag gcaaaacttt
    13741 cacttgttca atcgcttttt tctgtttgga caacgattat tttgttcgga agatttcttc
    13801 ataagaaagg gaatcatctt ctgctgtcag ttcatctagt agataacgga tttttttaaa
    13861 gtcgatgaac cgtgcttgcc gaaagacttc ttttccttga tgaaaaatca ggacaactgg
    13921 cgcagtcagc acttcaaatt tactagctac ttctggtgtt tcaaatgcat ccaaatgaag
    13981 tgccggaata tcataatgtg tgagaagttc ctctagtcgt ggacgaacag cgtgacaaac
    14041 actgcaatta ggcatcgaaa gataaatgac ggcaacggat gactgttgca gtatgtcttg
    14101 aacttcagtc aagttagtgg tttgtttcat ttttattctc ccttttatga tagaagtgat
    14161 atgtatgaga ccttaattta atgggaataa cagaaaaagt caattgaagt gctttggtaa
    14221 aaaataagag ttgtgtaaaa atttacagag caacttgttt tttttgaata aaagcgtata
    14281 tttgcaaagc gaatgaacta gaaataaaaa ggaggaaact catgttttct tttattccaa
    14341 attcggtttt gcagatgagt acgattttct tatcgatcgt tattgaagca ttacctttcg
    14401 tattattagg ctgtcttatc tcaggcgcct tacaagtatt tttgacccca gaacgtgtga
    14461 agagatggct tcctgaaaat cgtatcttct ctatttttac tggttcgatc ttaggatttt
    14521 tctttccttc atgtgaatgc ggcatcgtac cgatcgttca tcaatttgtt aaaaaaggcg
    14581 taccagttca cacagcgttt gcttttatgc tgactgctcc gatcatcaat ccaatcgtga
    14641 ttttttccac ttttatcgca tttggcaatt cttggcagat ggcaagctgg cgggtgatag
    14701 gcagcttttt tgttgccctt gtgattggtg tctggcttgc ctattttcaa aaagacgctg
    14761 tattgaaaga aaaaatcacg tctaaagtag cagaaaatca ccatcatcat gtccatgaag
    14821 aagaacaaag tgagaagaat gaaccaaaaa atcagctgat ccatcaaact gggcatgtgt
    14881 tgacacattc aatcgacgaa ttttttgata caggtcgtta tttgatcatt ggcggattaa
    14941 tcgcagctgc gatgcagact tatttaccaa ctagaattat gctaacgctc ggctcaacaa
    15001 agattttagc cattatcatc atgctgttat tggcctttac gatgtctctt tgctcagaag
    15061 cagatgcgtt tataggatct tctttgttaa gtctgttcgg cacagcacct gtagctgcct
    15121 ttttggtctt tggcccaatg gtcgatatca aaaatctact gatgatgaaa cgatatttta
    15181 caggacgctt tattttatcg ctgatcggac tgattgccct tagcgttatt ggattcacat
    15241 tatttattta ggagggaaaa tatgctgcgg tttttgattt tatctgggta tttcgaattg
    15301 atgatgtact tgcaagtttc tggcaaactc gatcaatata tcaatgtcca ttatcgctac
    15361 ttagctattc tttcaatgat tttatctgct cttttggctt tagttcagct gtatttgtgg
    15421 gtcaaaaacg gaaaagcaga agaaaaggca cacgagcacg atcatgacca tgggttgtca
    15481 aaaccttatc aacgtggaat cgcttatgta ctgctagctt tgccagtaat cgtggggacg
    15541 ttgtttccaa cagttagtct cgacactacg atcgtagaag ccaaaggatt taatttccca
    15601 gtaagtaaag aatcagtagg ggatccggag atgcagacac aatacttaaa gccggatacc
    15661 agtatttatt tcaacaaatc agactactta gaccaaatga atgagttgaa agaaaagtat
    15721 ggggataagc aaactatcaa gatcacagat gaaaactatt tagaagttat ggagctaatt
    15781 tataattacc ctagcgaatt cattggcaaa aaaatcagct atgaaggatt tgtttaccaa
    15841 acaccgaacg atagcaatac agatatcttc ttgttccgat tcggcattat ccattgtgtg
    15901 gcagattcag gagtctttgg tttgttgacg catatgccgg aaggagtaac ggttaaaaat
    15961 gatgaatggt ataaaataga aggaaccatc caatcagact attatcaacc attcaagcgt
    16021 gaaatcccat ctgtagttgt tacaaacgct gagaaagtct ctgctccgaa aaatcagtat
    16081 gtgtatcgtt ctttttaaaa gaacaaaata aataaacata gctcagacag ctgacacgca
    16141 aggacgtcgg ttgtctggct ttttttgcat gatgtattca tttgtttgat atttatttta
    16201 gaagcagcat cctgaaagta tttgatatcg gtttcaaagg tgttggaaaa gattgtgaag
    16261 agaattacaa tttattccta acatgtatgt tgaaaagttg tatgaaatga ttttgatagt
    16321 aaaatatcga gtggaggtac tttttatggt aacgcgtata tttattttgg atgatagttt
    16381 ggtttttgaa aaaatgctag tagaaatttt ggaagaatcc agaattttat taagtccttg
    16441 gaaatttgag atcgttcagg gattgaacgt tatacaattt gttgaattca ttaaaaataa
    16501 tgaaatctat tcctcagata tttttttcct agacatcgat ttgaacgcct attatagcgg
    16561 tatcgaacta ggcgaaatgc ttagagaaaa gaatgaaaag tgtaaaattg tttatctaac
    16621 gtctcatccg aataaagcaa taactgttat taatcgaaag attacgccta gtggttattt
    16681 gataaaagaa ttcacttatg aaaaaaataa aaaaagcatc caaagttact tattgaaaga
    16741 aaaagaaaac tatcctgttc aacatgctac gaaaaaaagg tggacaactg tccgttatgg
    16801 taatagcgat cactatatcc attacgatga aatcctttat ctaatgagca tgccaggtta
    16861 taaaaataaa ataggcctag tattgaaaga tgaagagatt ctcgtaaacg gcacaattaa
    16921 aaaatataaa aaatcactcg aatatgagta tttatgtaaa gatttaaaag cttatatcat
    16981 taatactgaa aacattacat ctattagcag agtaaacgaa atagtaacat ttgcaaatgg
    17041 agaacaacta gagatgggaa agagaagtat tgataaacta aagaatttta tgcaagaaaa
    17101 gatagaaaat gaatcatttt aaagaaaaca aagactcata tcttacctgt tagaagagtg
    17161 ttttcctgta gaagagaaca acccaagtag cagcctagtc gtttctatcc ttttttgtta
    17221 tgcactcttt ttgacattta ctccaccaat aacgaagggt acaataaaaa gtaataacaa
    17281 acaaaaggag tacagaaatg attgaattca tcaatgtaag taaaagttac gggaaaaaac
    17341 aagcattaaa agacttgtct ctctcgataa gacaagggga aatttttgga tttttaggac
    17401 ataatggtgc cggaaaatca actacgatta aaagtttagt tagtattatt gaaccatcat
    17461 cgggaacgat tttagtagat ggaatgaaat tgacggaaaa tcgattgagt atcaaacaaa
    17521 aaatcggtta tgtgcctgat tccccagata tttttttaca attgacggct ggagaatatt
    17581 gggacttgat ttcagctgcc tatgaattag acacacaaaa aaaagaaaag cgtttagcag
    17641 aattgacagc gctttttgat atgtatagtc atcaaaatga aacaatcgca agtttttcac
    17701 atggaatgcg tcaaaaaacg attttgatcg gcacgttgct gccagatcca gatatttggg
    17761 tattggatga acctttgcaa ggacttgatc ctcaagcagc atttgatttg aaagaaatga
    17821 tgaaggctca tgccgcaaaa ggaaaaacag tcattttttc tacacatgta ttggataccg
    17881 cacaacagtt atgcgataaa ctggctattt tgaagaaagg tgaattgatt tatcaggggg
    17941 cagttagtga attattgaat ggctcacctg atgaaacgct agaaaagatt tatttgaaaa
    18001 tggctggaag gcaagcgaca ggagatggac cgtatgtata agcgacaatt attagaatta
    18061 attcgagtta atcttttata tgttaatcca cagctcacga ataaagcaag agagaaagga
    18121 aaaaagggaa agcgattaac tatctatctt ttgtctcaaa atcttttttc agggctgatt
    18181 tttttactga tttacggatt tgctatgttt gctatgaact ttgttaaatt gccaggtttc
    18241 tttacttatt acgtggcact ttttggtatg ttaggtttaa ctcaaagtat atcagtcatt
    18301 tataatatct tttttgaagg aaatgacctg caagtatttt tgcctctgcc ttttggacaa
    18361 gaacaggttt tttcagcaaa gattttggta gttgctttga ccatcattcc ttttgttttt
    18421 ccgcttctag tcatgtttat tttaaccgga tggcgagctg atctagggat tttgcttact
    18481 atcattatct caatcctttt gttttctcta tttttgatta ttcttttttg tctggctagt
    18541 ttgattgttt ttggtttggc tcgaacagca gtgtttaaaa aacatagaaa aacagtttct
    18601 agtatcttgc ttggcagctc gatgctgatc gcatttgctg gaatcatgtg gatgaatcat
    18661 caaacgggga ctattgaaaa tgagttaaca gatcggcatc cgatttcgat attattgcca
    18721 ttctatgatg ctgccagtca gccactttct caacaaggtt tattgggttg gggaatacta
    18781 gctatcacgg ctctgggatt tttccttgct gtaaaacagc tgatagtacc aaagcttaca
    18841 gagcaactga ctgattcaaa cagtgagttc aaaataaaaa gaacccataa aaagaaccaa
    18901 aatgtgcatc agcttttatt tagttacaat gctcaattag taaaagaacc taatttgatt
    18961 atgcaagttc tttcttcttc cttgttaact ccaatcattt ttattcttgc ttttgcccta
    19021 ggaggcgaaa taaaattaac tgatttagat aataagttaa tcggtgttgt tttcctggta
    19081 gggatcgcat tggctttctt aacagttaat cagacttctt ttatcagcaa tcttatttct
    19141 ttggatcaag aaaattttct gtttatccgt tctttgccaa tctctatgaa ccaatattta
    19201 aaagaaaagt tcagattcgg atggatattg cagagttttt taactggtgg gatcgcatta
    19261 ttaagcggac tgtcttttca gctgcctttg ttttttatcc ctagcttgct atttggttct
    19321 ttattcggct gctacttatt gtcactacgt tatttcgcta gagattatcg gttgctgctg
    19381 ctgaactgga ccaatattaa ccaattattt aacagaggtt caggaagttt gggtttagtc
    19441 gcaactatga tgggttcatt gatcatcagt attattctta tcgtcttata cggaatgttg
    19501 gctttgtttt atccattttg gttagtgaat attcctgtga tcggtttagt tctaatactt
    19561 tccgctctat ggatcgttta ttatcaacga acgttttgga aaaaatttga ataaagaaga
    19621 ggggccgtga caaaaatcat gtcgcagccc cttttttaca gatagagggt gttcaaaagt
    19681 cagtttctca gtattaagtc aattttatgt aaaaatgtga gaagctattt ttacaaaact
    19741 ttttttaata ctcataatta cttcttattg ctcctgcggt tacttgtcgc aatgtttgtg
    19801 agatattttg tcatcgctca aggctgaacg tctctttcac aaccttttct ttgttacact
    19861 tcgtttattt aaaaagccgt ttttttcgtt tcatcatgat tgcgtcacgc tttactttga
    19921 aattacggat gttatggtta aagacttctg ataatacaag acctaaagcg attgctccag
    19981 caatcataat tgcttggacc gcataatgag ccgcttctcg ataggattca gtaacgagat
    20041 ttcgaaccgc ttgataagct aaaccaccag ggactagagg aaccatcccg ggaatattga
    20101 aaatcgttac gggcattttc aaacgtttag agaaaagatc gctgatgaat gctactccta
    20161 aagaaccaag caatgaacca aaagcagcag atccgcctat ttgaacacag atccagtaga
    20221 tcatccagcc aaaagcacca gaaagaccac aacaaatcaa tgaacgccta ggtacatttg
    20281 taataacggc aaatgccgca gtcgctaaaa aactaaaaga aaactgaata aatatcgtcc
    20341 ccatacttgt gttcctttcc taataaaaaa gctggaaaat aaaagcaatc gcaaaaccga
    20401 tcatcgtagc agtcatcatc gcttctgttc cgcgagaaac tcctgagaca taatgtccag
    20461 ctagcaaatc acggatagcg ttcgtaattt gaactccagg aactaaaggc atcacacatc
    20521 cgataatgat tagatcctga ttgcttccta agccaaaacg tacgctgtag atagcggcta
    20581 tgccaatcaa taaagaagat aaaaattccg ataaaaattt gattctaaaa aatttcaaac
    20641 tatatagata agctgcgtat cccaatcctc caatgactaa agtcacaggt aaatcggtca
    20701 tctttccgcc aaataacacc ataattgttc cactgatcac tgccgcgcta acgatttgca
    20761 accagacagg aaagaaagaa cggtctcggt cgattgcttg cagttcgcag taaagttctt
    20821 ctaatgttag ctctgaagca acatatttac gtgaaagatc atttactttt actacttttt
    20881 ctaaattaat cgaacggttg gtaatttgtt ccattcggat ggtcgaggta cgatcaagac
    20941 caataaagag accggtttga gtgacatagc tgaccaaacg gaaatttcca gaagctaaag
    21001 caattcgact catcgtatct tctacccgat acatctccgc attgctttcc atcatgattt
    21061 tcccagcgag taaacaagtg tccagcaaca gatctaattc ttttcccgtc atagcatctt
    21121 ctccaataat tgattgatta gttttatttt actacaaaac ataccagaag cgtaaagaat
    21181 aaaaaaactt gaggaaggaa taaaagaggc gtatactgtt tttgtattgc gggtgtagtt
    21241 tagtggtaaa atcccagctt cccaagctga tgtcgcgggt tcgattcccg tcacccgctt
    21301 aaaataggtt gtgaaaaagc tgctctgcaa caagtaataa gaacagccaa gcaaaaacaa
    21361 cttcttatgt ttttaacttg gatggactta ttaccgcaga tgcaagcttt tgcgcaccgt
    21421 ttaccaagcg gttgtgaaat cagtgtattg tcttgagtat gaagaaaatt tttgaataat
    21481 aataaatacc ttacagatag agtggtgact gattagagac gaggaatccg tctctttttt
    21541 gtatttttaa gccatcttaa aacaggtttt attttttgct gtttctattt ctttctagta
    21601 tttatttata ggggaacata tgttaagatt tagggagctg ttagtaaagg agtaaagaaa
    21661 tgagcaaaaa aacaaccaag gcgatcgtgg ggatcgtcgc tgctgtgatt gcaggggcat
    21721 tcggtgtttc caccacacaa aaagattcag atatctttca gcagattaca actatttttg
    21781 attccacacc acaagcttct caagtagcgg atgtgccaga atatgatgga aaacatcaag
    21841 aaatagaaat caatgggaat cagccggaat ttacaaaaga ggatcttagc ctttccaaag
    21901 gaacatggga aagctattca aatattgata aattgaacag agtaggacaa gccaatgcta
    21961 tgcttggaaa agatttgttc cctactgaaa agagagagcc tttgtatatt gacccgacag
    22021 gttggaaaca aaagaaatta agtgacgggc aatggttata taatcgcagc catttaattg
    22081 gtttccagct gactggtcaa aataacaaca ttaaaaactt gatgacaggg acacgttcgt
    22141 tgaatgctcc atacatgctt gctcacgaaa atgatattgc ggcatatatc aaagaaacaa
    22201 atcatcatgt acgctaccgt gtcactcctc attttgaggg gaatgaactg gtagccaggg
    22261 gagtgcaatt ggaagcagaa agcattgaag acaaaaaaat tgaattcaat gtctttatct
    22321 acaatgttca agatggctac acgatcaatt atgagacagg acaagcaaca aaaacaaaaa
    22381 attagtagca gctattcgaa ttttttatga atattatgaa aaaattgtgt aaaaacatac
    22441 attcaaagaa atatagcgta tactttatga aaagagtact tttgataaag ctacagaaaa
    22501 aaggaaaaaa acatacaaat agtgtttctt aatttattcg gaagtttgga atatttgttt
    22561 gtagttttcc ctttcttcga gtaaaataag gttgtagaag gtaagctgga attaccttgc
    22621 gggaaagagg tgtagaatca tgggttttac agatgaaacc gtacgttttg gttttgacga
    22681 tagtcgcaaa aaagaagtga gcgaaacatt agcgattgtt tatcgggctt tggaagaaaa
    22741 aggctacaat ccaatcaatc agattgtcgg ctacttgctt tctggagacc cgtcctacat
    22801 tcctcgttat cgtgatgctc gaaatttgat tcgccgtcac gaacgcgatg agatcatgga
    22861 agtaattgtc aaggactacc tctcaaatca tggagtgaat ttatgagagt aatgggcttg
    22921 gatgtcggtt ctaagacagt aggtgttgct atcagtgacc cctttggctg gacagctcaa
    22981 ggagtagaga tcattcggat caatgaagca gaagaagagt ttggctttga tcggttgaag
    23041 gaattagtag cacaatatga agtagaccgg tttgtagttg gattacctaa aaatatgaac
    23101 aattccatcg gaccaagggc agaagcttcc atgtcatacg gagaaaagat caaagaacta
    23161 ttctccttgc cagttgatta tcaagatgaa cgattaacca ctgttcaggc agaacgtatg
    23221 ctagtagagc aggcaaatac ttcaagagcc aaacgaaaaa aagtcatcga taaattagca
    23281 gctgtgatga tcttacaaaa ttatttagac ggctcaggta agcaattatt ttaataataa
    23341 aagaaaagag gtaatcttat gacagaacat aaccattccc acgaccatga aggacacgaa
    23401 cacatcacat tggtggatga ccaaggaaat gaaacattat atgaaatcct actaacagtt
    23461 gacggacaag aagaatttgg acgtaactat gttcttttat atcctgctgg tgtttcagaa
    23521 gatgaagatg tagaattaca agcttatgca tacatcgaaa acgaagatgg aacagaaggc
    23581 gagttagaac aaatcgaaac agacgcagaa tgggacatga ttgaagaagt attcaataca
    23641 ttcatggcgg aagaagaaga atagactata aagctaggag aaaccttgat atgatagggt
    23701 ttctcctttt tttatttttt actgtatgcc atattgtatg ccatctagtc aatatttaag
    23761 tgttgtacta atttttcaac agtcttttct ttcgtttctt ttgccaagtg accgtaacaa
    23821 tcgatcgtct gactaatttt tgtatgccgt aatctagttt gaacttctac catagtagct
    23881 ccagcgtcca agagcattga agcatgagtg tgtcttgcta catgcacacc gagaaatgga
    23941 accatagctt tttcacaaat agtaatcaat cttgcataaa tagcgtggtg tgtgattagt
    24001 tctttataga taccacaaaa aatcatagtc ggatcagtta caccaagtgt cagcatatat
    24061 ttccgctgat taatctgcca tttttttaat atagaaattg ttctttcatc tagtggaaga
    24121 accccagcac tacaatcagt tttaggatca ctgattttat agccattttt ggattggctt
    24181 agtgttttat tgatggtgac cgtattttct tgaaaatcaa tgtctgacca attcaatgca
    24241 agggcttcac tgatcctaac cccgctataa aagagaaagc gtagtaacgt tttatcaaag
    24301 tcgtttcgat aagtttcagg tttgctgttt aggtaatcaa acaaggtgat cagttgttct
    24361 ttactgtaaa ctttgacctt ttctttatgc ttagtggtgg ttcttttagg cattaatacg
    24421 ttgtctagtg gattagtctc aataatccca agattgatag cgtagctaaa aatacgctta
    24481 gaagttgaat gaagtacctt gtattggtca gtagtttcag cccagctatt tacaatttgt
    24541 tgggcttttt ttaccgtgac ttcattcaca cgaagatttc catattttgg aagaatccac
    24601 tttctaaact tgatttcagt tttcatgaag gtggcttctc ggacagtggt tttatatgtt
    24661 tctagccaca agtcataaac ttctttaaac gtttcgttgt tcgctttttt aataccgttt
    24721 ttatcgtagt ccacttgcaa gcgtgacaag gcaagctggg cttctttttt tgttttgtaa
    24781 cctcgtctac gaaccttatc aggtttgcca gtcattttgt tcattccaag ataggcttca
    24841 tagagccaaa atttaccttt tttagtttca tattgcttga atgttgccat aatagttttc
    24901 cctccatagt gtagcaagcc gtgggggcgg ttggaggagg cagtttatta ttattttgaa
    24961 tagtgtttat ttagctcagt aacaagagct gtgaactctt ttagaagatc attttcataa
    25021 aattcttgat actctttttt ctcatctgta tttttttctg aatcattaat taatgagtca
    25081 tatcgtaaaa gcgtatttag aagagctgcg agataagcaa ttggggtact cccgtcagga
    25141 aattcaattc gagatcctgt tttaagatat tttattgcgt ttgttaaata aatcttctgt
    25201 gagacagtca ggttatcttc ttcgatgtta ttgatagcat tagaaagatt agaatcaatt
    25261 atagagttat ttttttcaat agtttttttc aattgctttt caagcacatt tcgcttaatt
    25321 tcgggcatca tatgcatatc tcttaatgta taagatccgt ttaatagata ggtagtggat
    25381 acatctaata cctttgaaag ttgtttaagc ctggtgttat tgggaagatt atagccattt
    25441 tcccaattac taacggcacc tttactggcc ggtggggata atttacctcc aagttgttcc
    25501 attgagagtc ccttttcaat tctagccagt ctaattcttt gtcctgtctc cactgcttcg
    25561 gggtattttt ttctagccac attttcacca ccattcatat attttctcta ctatatcacg
    25621 atttaaatat gtgtgaaaca taaaagttca aaaaagtatg ttaaaattct tgcaaatgaa
    25681 aaggtatgat ggtatattga gtttataaga ggtatagaaa tgtattcaag agcctcttgc
    25741 atttttttaa aatctttgta tagaaatgta tgctaacgag gggggataaa tatggaatta
    25801 aaaattgaca aactcacgct tgatgatcca gcttttaaaa ttattgacga tcgagttgtt
    25861 caacaagtag agcaaactca aggaacgtta ctttcattat tagaaaaaaa gtaccagttt
    25921 ccaaaatata tgaacaaggc acaagctgct aagtatatga acgtttcata caatactatg
    25981 atgcagaaat atgttcctaa cggtttaaag ttgattatag ttgatggagt cgtaagaatt
    26041 tcacaagatg aatgtgatcg ctttatggaa gaataccaaa aataaacgca agccgtgggg
    26101 gcaaagggat taataggagg aaataataat ggaatcgaca caaaataaag caatcgaaaa
    26161 agtattatca acaattctta cagaagacac agcggcagaa ttagcaaact tggaaggcaa
    26221 agcgctagaa gaaacgtttg aatgtttgta cgaacaaatg gactatcaaa aattgctacc
    26281 acaagaacca acggtaagcg gagtattgcg tggcttgaac gatttagtac aagcagagtt
    26341 taaagaacga ctttctattg aagagtatca agaaattttg tatcagcaag ttgagttgtt
    26401 agcgagcttg ctagggcttg aactggagga cgttgaatga aaactgaaat ttggaacgga
    26461 cacattataa gatttgtcga tattaatgat gaatggtggg cagtggcgaa agatgttgcg
    26521 gaagcgttag gtcttaaaca ggttactaga gccattcact ctttgcctaa agatggggtt
    26581 actactagta aggtcatcga ttcattgggc agaacacaag atgtaaacat catcaacgaa
    26641 aaaaaatatt taccgcatgg cattcaaaag ccgtaaaaaa gaagccgagg catttcaaga
    26701 ctggattttc gacatcatca aagaactacg ccagtccaca ggacttgaag gctttcaagt
    26761 attccgcatg ctagacaaag aacaccaaaa agaagcaatg actaaattaa gccacgctat
    26821 tactgaacca aaaccagtag actacattaa agcgaatgtg atagccaata aagctgtatc
    26881 aactatttat ggtcattcca aaatggtaaa aaagaaagat atgactcctg aaatgttggt
    26941 tgatcgtgaa cctattttag atgagacagt agaattaatg actgttaaag aaaaatatgg
    27001 gttgcagttt tcagtgagtg agaaaattta taatcgttct gctgaattgc agacaactta
    27061 ggagcattaa catgatcgag aacaaagaaa aagccttatc cataacagtc ttggcggaca
    27121 ggataaaggc aacttaattg ggataaaacc cgatattctt tgttccgatt ataccatatt
    27181 aaaaaatcgg agggaaccat tatgaaacga aacttaatta taggaagcat tttaatcggc
    27241 ggaattttcg gcttattacc attaattgca aaaatagcta tctttgcagg attagcgtta
    27301 tatctcttca ctgcttatga cgagtacgac tatcaattaa gaactggagg gcataagtag
    27361 atgtacaaaa caaaactatt aaaccaactc gatagccttg aattagagga aattaatcaa
    27421 gggatcgctg aactagaaaa taatatcggg aaaacctact ttggaaattc attcaatgaa
    27481 aaactaacag ttttgtatgt actcaaaaaa catgcagaac acaaaataat ttgtcgagaa
    27541 atcaacgagc taaaaaatca gattttaacc gcatggttaa acattactga tatgcaagaa
    27601 gcaagggtga aaacatttaa tacatgggta aagtatcaga atcaattaaa aggtgctgaa
    27661 tttgttcggg atgggttgaa atacgaactt gagcagttga agctcatgga ggtgtctgaa
    27721 tgatttatac cagtattttg aaaaaggagg agaggtttat tggcaaataa ttatcaagca
    27781 gcaacggaat tacaaaagtc gggatggtca atttatccct tatctcctgg aactaaaatt
    27841 ccaataaaag gctctagtgg ttggaatgat gcaactaacg attctaatca aattaatgaa
    27901 tggtggacgg aagatgcaac tagaaatatt gggttaatgc ttgaagatca tcaaatggtg
    27961 gtagttgacc tagatcaaca ttctgaaaca cgaaacggcg ttgagaatta taaaaaactt
    28021 gcttcaatgt atgatccatt tccttctact tatacggaaa tcacaccagg taaaggaatc
    28081 catttcttct ttaaaattcc tgatggtgta attatccaac aagaaacagg tgcatttgct
    28141 gatatcttcg ggcgtgatga taaaggtaaa agtttaagcg ggattgatat tatcactaaa
    28201 ggagttccaa tagcgccaac tatgattatt tcaagaagtg ttgggagagc atatgaacct
    28261 attgaaaacg tatctctaaa taatattgca ccagctcccg attggctagt gaacctactg
    28321 ctaaaaaaac cgcaagaaat aaagactcaa taccgacccg tagcaaaaac agggacagca
    28381 aaaaaattag atatgattgt tcaaggcgct ggcgaaggta gtagaaacga tcatttaact
    28441 aagttatgcg gttggttact atggcacggt gtagataatc aaactttagc agaattgatt
    28501 tatacagcaa atgcgtacaa tgaaccaccg ctaaaagata aagaagtgaa tcaaattatt
    28561 cgatcgatga ttaaaaaaga tttgaggggg aaagctcatg gctcataagc ataaattaga
    28621 tagtatttta gactttcccg aagccagcga gcgtgaagac aatatcattg aattgaaaac
    28681 ctggatgtca cgattaagat gcaataaaga cgatcagatt aaaagtaaca gtgttgtgaa
    28741 tgcagaattg attttaacca atgatagcaa tttagctgga acaattgctt ataacgaatt
    28801 tagcggatac attcacctat taaaagattc tccttggatc aatcgtaccg ctggagaatg
    28861 ggaagacagt tttgaggatg ccttaacggc atatattgaa gaaaactaca atgtagtttt
    28921 tgatgacaac aaaatacaca aagcagtagt aaatgtggct agaaagaatg ttttcaaccc
    28981 agtaaaagaa cgtatcgaaa aagttaaatg ggatcaacag cctagactgg aaacactgtt
    29041 tattgacctt ttaggggtag acgacaacct ttacacacga gaagtaacaa aacgttggat
    29101 agtcggatca gtcgcacgta tctataaacc aggaataaag tttgagatcg ttccagtttt
    29161 ggatggaccg caagggattg gtaagagtac cgttccagca ttactttaca cggatgattt
    29221 tttcaccgat tcattagatt cgctaggtga aaagaaagat gattatatgc agcttcaagg
    29281 caatgtaatt attgaacttg gagaattatc ttcaatgaac aaaactaaaa ttgaacagat
    29341 aaaaaacttc atcagtgcca agatcgataa aattagacct ccttatggtc ggaatgtcat
    29401 agcttgggca aggcaatgtg tatttattgg aacgtcgaat gatgggcagt atttgaaaga
    29461 tgatacagga aaccgccgtt tttatccttt gccttgtaag aataagccaa agatgaatcc
    29521 gtttaaaaca aaggatgaat attttcttca agtgttagct gaagcaaagg tgctttttga
    29581 taaaggacaa cgaatctatt ttagtcccga tgaagataaa gaagtcttag aaattgcaaa
    29641 agactatcaa gaggatgcca agatggagaa tccaattaaa gaagcaatca ctaaatattt
    29701 ggaaatggaa atcccgtata aatgggaaga agctccagcg tgggcgagac gttcttatta
    29761 tcagaactat cctgacagta aatgtaatga gaaaaattta cagcatttct ataatggagc
    29821 tactatgcaa caagagtttt atttagttga tagcgtactc accgctgata ttttggaagc
    29881 tgtttttgat aaacaagcaa aggatttatt gaatggtcga tcagatgcag aaactaaaaa
    29941 gattgccttg attattagca gtattcctgg ttgggaacgg aagcaactgc caaaaagaaa
    30001 taaacgacga ggattttaca ataagtcaaa tgcctatgaa aataaaaatc gagctggaaa
    30061 gtacaaaaag taacgggtag taacgggtca gtaacaccta gcccgttact agtttaccct
    30121 tactgcccca agggatacag caatttggta acggggtaac accttatttc cttaaaactt
    30181 ttgaaatagt tagctaatat gctggggaat aatgttactg gattttaccc gttacctgtt
    30241 accaaaacgc tagaaccctt gtccccgttg ggattgagtg gtaacgggta cctgttacgt
    30301 aggtgttacc acgtgttacg aatcaaggag gaaagtaaat gctcaatatt atttcaacaa
    30361 acaaagcacc gaactttcaa tatacagatg aaatggatcg gtttctaatg aacacgctgg
    30421 catttagtgt gggattggta acagaagatt attccacatt tgatccagaa gtattaaaga
    30481 taatggaaga agaacctgat tggctgcagg agtcagtagc gtggtgtcaa tcgctagtag
    30541 tgggatcatt ggtagatagt ggcaactatg atgatacagg tgagctaatg gatgaattta
    30601 attgtctgct taacctatat gatcgagcga gacaacgaga gcttacatcg aatgaggata
    30661 atttgttttt aaacatccat gataagttct tggctttgct actaacagat gatgaattga
    30721 taactaattt attggaggta gaataattat gcatatggaa agtattagat acttcgatgg
    30781 tgagaaacac attgagtatt caaacactca agaagatgta gactttatta gttttgagga
    30841 agataaaatc actaaagtta attttaaaga cggaacgtgc ttgaagatag tttcgcctta
    30901 tattgaatac aaatcaaaat gggaacaaga tgattctgac gaacgagatc gctttggttg
    30961 gtaatctatg gcagttaaaa aacaatgtaa tcatgctgga tgtaaaacat tgattgatta
    31021 taggcagaag tattgtggga agcataaagc aaagcaaacg gcaattaagc gtgaagaaag
    31081 aaagcaaagc gaaggtaaat attttcaatt ctatcaaagt agaacatggc gaaaggcttc
    31141 atacttgtat cggttaaatc atccagtatg tgaggattgc ctagaagaag gcttgataag
    31201 gaaagctgat gttgtagatc acaaaattga actaaaagat gattggaata gacggttaga
    31261 tgaaactaat tttcgttctt tatgccatgc acaccataat tccaagacag ccaatgaaag
    31321 acaaagacga gaaaagagta ccctttgagt taagggggca tagtgtgaaa cttactgaca
    31381 atcgatgcct actcattttg gtacaaataa ccgttgtgaa aaggcataag ggtaattaca
    31441 aataacgatt acacttgtaa tcacaagtga aaaagtatat aatgaaagta ggagatttat
    31501 tgaaaaatac aattgttatt actgataaaa cagggaaaca gcgcaccatt gagttacctc
    31561 attttggtca agtgaccatt caaatgcaga acggaaaaat catttatata gataaattag
    31621 ataaagaaaa attctgatcg aaaaacgaag gaatgtgagc ttaattgctt acgttccttt
    31681 ttcttttgtc tgaaaggagg acaacatggg acaccctaaa atattacacg atacaaaagg
    31741 caatatatca agtgaagaaa aagcaatccg tgtggacgct agagaagaat tgttcaagca
    31801 gcaaccatta ataaatatca cgccccctga ctggatggca gcgagcgcta gaagtgaatg
    31861 gaatcgtatc gtaccaacat taaaaaaaga ttatccattg agtgaagcag attatggttc
    31921 attggtagcc tattgtttag cctttgctcg aatgaaaaca gccgaagccg agataagaaa
    31981 atcaggtacg tttatcacat gcgaaaacgg agtaaagaaa gccaatccag cggttcgggt
    32041 tcaatcacaa gctatgagtg atttgaaaaa acaagccacc tcactaggta tgaccttaga
    32101 atcacgatca aaactagctt tgaacaaggt taaaaatgat gaacccgaag acccgttcaa
    32161 agagttgatg aattcatgaa tgattacatt gagaaagtcc tatcaggtaa gctgattgca
    32221 cccaagaaaa ttatccaagc gtgtgagcgt catataagcg atttggagcg ttctaagtca
    32281 gatagcttcc cttatgtgtt cgatgaagaa caagccacca aagcgattaa attcattgag
    32341 ttgctaccat ctacggacgg taaagcaatc aagatgttag gatttcaaaa attcattcta
    32401 ggcagtcttt atggctggcg tactaaagaa gggaattaca ggcgattcaa tcgagcgttt
    32461 accagtatga gccgtaagaa tgggaaaacg tatatcgcaa gtggcatggc tgccaatgcg
    32521 ttgattatgg aacaagaacc agcagaagca aggcaagtat tgtttgtaag taacgctttg
    32581 aaacaagcta aattgggcta tgatatgctg tctaattcac ttagaaacgt ggtcaagtct
    32641 agtaagtttt taagaccaca actgaaaatt atgaactcta agattcagca cttgccctct
    32701 aattcgttcg ctatggcact ggctagtgaa accagcacgc tagatgggtt tgcaccaaca
    32761 accgcaattc ttgacgagtg gcacgaagca aaaactcgta aaacgtacaa cgtcattaag
    32821 tcaggaatga cccaacaaaa gaacggctta ttgtgtgtta ttagtaccgc tgggcttgat
    32881 ttaaacgttc ctatgtacga ggaatactta ttgttagatc gtgtgctaaa aggcgaagaa
    32941 caagccgaca ggtactttat agcgatatgg gaattggacg atcccgaaga aattcacgat
    33001 caagagaaat ggatcaaagc caatccgatt tttgaaagtg aagaaatcaa aaaagtaatg
    33061 attccaacca ttcaagatga tgtgaacctt gctttgaaac aaaataacct taattctgta
    33121 ttggtgaaaa acttcaattt atggagacaa gcgagtgagg acagctatat gattgctgaa
    33181 gactggcagg caaccgaagt agaaccacaa gatattacag gcaagcccgt ttatatcgga
    33241 gtggatttat ctaaaacaga tgatttaact agcgtttcat ggatcgtacc gctagataac
    33301 ggcgaacttt attgtgattc acatagcttt gtagccacca aatatgggct tcaagacaaa
    33361 gaaaagcgcg acggtttgcc ttatcgagaa ctagaaaaag ctggtgagtg ttccattact
    33421 caattagaaa gtggaatcgt ggactatgac caagtatttc agtttattca agatttgatt
    33481 caagaaaatg atttggaatg tatggggatt tgttatgacc cgtataacgc taattcgctt
    33541 atcagtaaag ctgaaaaagc caactaccca atgttagaag taagacaagg aacgattact
    33601 ctaaacgttc cgacccgaac ttttagagaa caagtttatg aaggcaacgt tattcacaat
    33661 aaaaatacga ttctcaccca tgcagtgaac aacgctattt taaaaacgga taacaacggc
    33721 attcagatta ataaatcaaa gaacagtaac aaaattgatc caatagctgc attaatcaat
    33781 gcctatgtgt ttgcaatgga ttacttcacc acaacggaag gagcgaaagc agacaatgaa
    33841 ttttatacaa gtgaagaatt ttctttctaa ttacattcat accgttcttt tacttcttgg
    33901 attggtgtgt gtgttggttg ctatcacctt actaacaaat gtctactatg gcttgttagc
    33961 gctgggcata gtgcttattg gggtagcggt catgctaaat acagaacaga agggaggtta
    34021 aaagatggca ttttttaaag cgagacaaaa tacaacggga gatcctttcc tacaacatgt
    34081 ggtatcaatc caatcggatg attacaccac cagctttaca agcgttcgtg cattaagaaa
    34141 tagtgatgtg tttgcagccg ttcgaattat tgctagtgat attgcttcaa gtccgattca
    34201 attggtcaaa aacaatatgc cacaagctga taatgaactg gtgaagttac taaacgagaa
    34261 acctaattca gaaatggatg gttggcattt aaagtttgct ttggcggtca atatgttgct
    34321 aaacggtaat agctttgcag agattaaacg caacggtgaa aaggtagaag aaattcactt
    34381 attacctaac tcaagtgtaa cggttactca attagataat ggtacgttgt cttatcagat
    34441 tggtgataaa aaaagacgtg tgaagtctag cgatattttg cacttcaaat atttcaccca
    34501 agatggttta acaggattac caccacttta tgcgttacgt gatgaactga aaattcaaca
    34561 agctggcaac cgcacattgc acaatttctt tactcgtggt gtcagtgggt caggtatttt
    34621 gaaggttcat aagtcagatt tagacggatc agcaaagaac gcaattcgtg aaaagtttga
    34681 agaagccaac ggttcaagta gtggagataa tgctcttaga acgatcattc ttgatgaaac
    34741 aatggactac aaaacattag aaataaacac cgatgttttg aagcttatca attccaatga
    34801 ttggaacaca aaacaaattg ccaaggcgtt tggtgtaccg attgagcgtt taggcgttga
    34861 aaatgaacat tctagcaccg ttcaaagtaa tctccagtat atccaaagta cgctgattca
    34921 ttactttaat gtgtttgtga gtgaattaga tacgaaactt gaaaccaata tccgctttaa
    34981 ttccgatcag ttactagaga ctgatcctga aacaaaagta aaaaatgtat tggatcaggt
    35041 caaagggtca cttctcacga ttaatgaggg gcggtcgaaa atggggctac cccccatgga
    35101 tggtggcgat cgtttactag caagtttgaa ctttacgtat ttagatacgt tggagaaata
    35161 tcaattaaaa gaacaggaag gagttacacc agttgaataa tgaagaagaa aaggaaaaac
    35221 ggctgacaga agaagctgag ctaacagccg attctcccaa agtagagaaa gaaaatgaaa
    35281 aacaaccaac agacggcaaa actatttcag gctatgcgtt gaaattcggg caaccgtcaa
    35341 aagatttagg cggctttgta gaagtgatta cacccgaagc attaaaagag gtggatttat
    35401 caaatgtgtt cttattatat ggacacgatt attcaaagcc actagctagt gtcaaagcag
    35461 gtacgttgaa attgaatgtc gatgatacag gattacattt tgaagcagag ctaaccgaca
    35521 cgacctattc aaatgatgtg tatgagaata tctcaaaagg ggttgttgat tctatgtcct
    35581 ttgggttcgt gcttggatta gattcattcg acaaaaaaga agatggcaca attgaacgat
    35641 caatagataa aatcaaagca cttaatgaaa ttagtgtcgt gaccgttccc gcttatgatt
    35701 catcaaatgt ccaagtaaat aagcgttctt acgaatcgtt tatgagtaac aaccaagcaa
    35761 aacaaacaaa caatagctta gaatccactt ctaaagcaca aaaggagagt aataacatgg
    35821 aaaaaacttt aatcgataac gaaaaaacag aattgcgtgg gtatgaagaa tatatccgtt
    35881 cacaaggcga agtgcgtgat ggagtcacta ctgtaaatgc agcggcagtt gttcccgaag
    35941 aagtaatcgg tgaagtcttt gatttgaaac gttcaaatta taacttagct caatatgcaa
    36001 cagtaaaaac agtatcaaat ggacaaggta aatatccagt agcaactaac caacaagcag
    36061 tgttagcaac aaaagctgaa cttgctgaaa ttggtgatat tgacgctgaa atgtttactt
    36121 cagttgatta taaagtagaa actcgtgctg gtaagattgc cttatcaaat gaggttgtgg
    36181 aagattcagc agtgaatatt gtacaagagg tcaaagatca gttagcgaaa ttggtagaaa
    36241 acactgacaa taagcatatc atggatttat taaaaacatt cactaagaaa acggctgcta
    36301 cgttggacga tttgaaacaa ctatacaatg tggcattaga cccagcatta aataaaatgg
    36361 taattctaaa ccaaagcgga tattaccacc tagatacatt gaaagattca gatggacgtt
    36421 acattttaca acccgatgtg acagcaccta gtggtaaatc attattcggt atgccagtag
    36481 tattgattgc agatacattg tttgccaatc ctaaagctgg aacattccct atgattatgg
    36541 gggatattgc acaatctatc tttgttgctc gtagaaatca agtaacgact caatgggaaa
    36601 aattcgatta ctactcacaa ggacttgcag tgatcgttcg caacgattat aagaaaattg
    36661 atcaaaatgc ttcagtgtat attgagttta cgccagttgt aacaccaaaa gtatagaaaa
    36721 atattgggcg gcggtttatc ccactgcctt tttttcatta ggagagataa aatggtaatt
    36781 ttggatagca taaaaaaaag tatgcggatc gatcacacta ttgatgatga ctttattcaa
    36841 caattgattg atacagcagg cgaatatata aaaagtgcta ttgatagtag cgcaacggat
    36901 aaagatatgg ataattatca gcaatttgat ttggcggtgt cattacttac tcaacattgg
    36961 tatttgaacc gtcaagaagc cagcagcgaa cggataccag taacggtaca agcattagta
    37021 caacaaatga gaggtgctta ttatgccaat cattaagaat gtaaatgaat tgactgagag
    37081 agtcaagttt aaaaagacaa aacgggtaaa agatgaagat gggcagatgg tggatagtga
    37141 agaaactgta tttgaatgtt gggctaatgt gcgttcacaa atgctaaaag acgtgcttgc
    37201 tagtgtaggt accattcttg aaggaacgtt gacgtttatt attcgatacg atcaagatta
    37261 tgaactaaca aacgatatga aagtagcttg gaaaaacaag agttataaaa ttatttcaat
    37321 caatgaagga acggcgttta aagattatac aacgatcata gctaaacaga tttcttagaa
    37381 tgattacact tgtaaacgtt gtgggtattt agtataatta aggtagtaaa tgaagggtta
    37441 gctgcctgaa ttttgcaaga cttagctagt cgaaatctat tgttggactg aaaattgtag
    37501 gtgtggttgc aaacatgctg gactaagaaa gatgatagat gatgaacgtt caattttaat
    37561 acaggcatgc taggactgat tggatggttt tggcaagctg attgtgtaga aatcaaagaa
    37621 cacgtctgtt gtggacgtgt tttttttgtt ataattcaaa agaggtgatt taatggacaa
    37681 agcagagttt acaaattttt attggaagta ttatctacat ttagaaaatg agtttattaa
    37741 taccacagat tttgtgatgc tcgatgagaa taatttcaaa acattctcta ttgaatatca
    37801 aaaactacta ctagcgattg gttcggagtg tgagattatt ttcaaagaac tttgcggatt
    37861 tgatagcagt agcaataaaa gcataactga ttttaaagct gttattaaat ctagcgaatt
    37921 aatgtgtttg gataatggtg ttagggttat gaattcttta acattgactt cactaaaacc
    37981 ttttggagat gggtggcctg aagatacacc agattggtgg aaaatatata acagagtaaa
    38041 acatggtcgt tcttcaaatt ataaggaagc aaatttggaa aatgtgttgt atgctttagc
    38101 aagtctatat ttattagagg aatatttgca taagaaagta atactagaag gtgaagtaga
    38161 ctttataagt ccagagtcaa atctatttac gttatcttgg gaaagaaaac attcgtcgat
    38221 gcaaaaagtg acgtttgaaa aagtggattc aaattatgta gctccactca ttgattttaa
    38281 agaacaagaa gaaatttaaa aacccccacg gcttattgaa gtttttgtat gccatattgt
    38341 atgccatttg gtttaaatcg cttgaaaaat agtgatagat aactctaatt catttagtca
    38401 taaacgctgt tttgataacc tttatagttc tttgatatgg gtttctcatt catggcggaa
    38461 gaagaagaat agaacaaaag aatgaattaa taagatataa aaaaagggat tgtgacaaac
    38521 gtcttgtcac aatccctttt tccaaataaa cgatgttcaa tcagctgccc ctcggcattc
    38581 gctcgaaaaa cggcgaaata tgaagaacta ttttgccatt tgtttctcta ataatcaggt
    38641 caaaacgttg acttcacagt ctcttcctta aacggtgttc tcctgccaac tcctcggtca
    38701 caagtcacaa tattcaacaa acatgagaag ctgttttttg aaattgttcc ttgtgactcg
    38761 gagctgaacg gcaggctcac gacctctttc ttaaacggtg ttcaaaagct tgcatctgcg
    38821 gtaataagtt caaccaaacg aaaattatga ggagctattt ttgtttggct gttcttatta
    38881 cttgatgcag gacagctttt tcacaacctc ttttcgttta gcactaaatt tccttaaaaa
    38941 gtagaaaagc tttcgaagcc tcgtttgttt cttttactgt ataataaaag aaaagagtaa
    39001 caatcgctaa aagatataaa caagagaaag gaaatacaag atgattggtc tgatttattt
    39061 tgtggtgatc gttctggcga acacagttgg cgctatttcc gggatgggtg gaggcgtgtt
    39121 gatcaaaccg attcttgatc ttatcggcgc ccattctgtt gcagggattt ctttttactc
    39181 aacagttgcg gtattcacga tgtccattgt ttcgactgtg cgacaagtat caagcgggaa
    39241 atcactgaat tggcagattg tcggttgggt atctagtgga gctgttgttg gaggagtagt
    39301 cggcaacatt gtattcgaag tttttttaca gttatttgaa aacgagaagc atgtgcagct
    39361 gatccaaatt tttttgacgg tattgacctt ggtttttgcg ttcttctaca ccaagcatca
    39421 tcagccaaaa tttcatttga cgtcatggac ttggtacctt atatgtgggg gggttctggg
    39481 ctttttagct agttttttag gtattggtgg tggaccagtc aatgtttctt tgttgatgct
    39541 gatgtttgca ttgccaataa aagaagcaac actatattct ctatctacga ttttcttttc
    39601 tcaactggca aaattggtaa cgattgcttt aacaagtagc tttatgcgct ttgatttatc
    39661 tatgttgttt tatgttatcc ccgctgctgt tattggaggt ttgtggggag caagatttag
    39721 cagggttttg tctccaaaaa aagtgacctt tatttttcaa gtaattgtcc tcgtggttct
    39781 tttggtcaat tgttacaatg cctttgtcat cttacaaaca ttaggagaat ctagactaac
    39841 gcaagtcaaa atcactgaaa catattgttc taaatgaaaa aaggagtata ctgtgataaa
    39901 aaaattcatt taaaaaaata aaaattggct ggtgatttgg atgttgcggg aaatgcgttt
    39961 aggagatgca gaagcactac gagaaatcaa tgcggaacaa ttaggatatg atgtaccatt
    40021 aaccgttacg gtgagacaaa tgaagaaact gttgaaggag ccagaaaaaa attttttctt
    40081 ggtctatgaa gatcctactt ctaaaaaagc agctggctat gtacaagcgg agtcctatga
    40141 atcgattttc agcgatccga tgttcaatat tatggcactt gctgtgtcta aacatgctga
    40201 aaaaagagga atcggaaagg ccttgatgac tggcgtagaa gaagcgaaaa gacgaggaat
    40261 atcagcaata agacttaatt ctgcagaata cagagtagaa gcacaccgat tttatgaacg
    40321 catcggttat cattccgata aaatgcaaaa aagatttttg aaaatcctgt aaatttttat
    40381 caaaaaatac ctggtcatca agccgatact tgatgatcag gtattttttt tgactaagaa
    40441 agtaattgca tcaagaaacc tgagatgatc aaaacgatgg caccaccaag ccgattgccc
    40501 atttgagcaa aagcaataag ttccatccga ttagctgcag ataagacagc tacatttcct
    40561 gtaccaccca tcgagttgtt gcataagcca gcagtaatag ttgattcgat tggatataaa
    40621 ccaaacaaac gaccgacaaa tcctgaaact actgaaatcg cgataacact tgtcaaacat
    40681 aagacaacaa attgccatgt caaagcagaa gccaatacat ttagattcaa taacgcaata
    40741 ccgataccgg caagaacagc tgctgtcaag tttttgacaa tcaagttgtt gaacatgata
    40801 gctgcatctt cgtaatattc tggtaatagg tttgcgattt tacagataac aacgataatg
    40861 atcatgaatg cgtaagcatg gactttagga acaaaatagt tacagatttg tcctaagatg
    40921 aagaaagtca atgataccat caaaccgaca cctaattgtg gaatggaagg ttttacattt
    40981 ctaggttttc catcgccaag gtcgcctttt tccattaatt taccattacc gttgaatttt
    41041 ggcattgatt gtcccgcacg agcaactaac gctgcaccaa tgatagctag gacatttgtc
    41101 attgctgtcg caggaatcag gcgggaaatg atagaagaag cgtccgtacc taaaacattt
    41161 gcataaatcg ttgataaagg tacagctccg gctccaacac caccagccat tgcgggcaat
    41221 gagatatata aaacggaatt tgcaaaacca tttccgataa gcatacctac gagtcctacg
    41281 gcaaaaaagg agacgaccat tgaaataagt gcaacgggaa taaaacgaac agaagctttc
    41341 atcaatagat tacggttcat accaaggatg ctaccagtaa tcaaggcagc aatataaaag
    41401 tctaaaaagc ccatattgct gacaaagttt tctgtggatt ttactacata ctcaggtaag
    41461 atcccaaatg tagcaatcgc ggcagaggca aagatcgcaa aaacggagcc accacctaaa
    41521 taacttttga cgatagggat ttttgttccg ataaaatgaa ataaattacc gaaaatgacc
    41581 aaaacagcga ttgggccaat catattcgtc ggtaattttc ctaaagccat tgcaatgatc
    41641 aggacaagga ccatgatcaa ataaaggggc aaacttacgc cggtaatttt tgttgaccaa
    41701 aacccttctt ttttcttttc ctcaactgca gttgcggttg ttgttttttg tgccattttt
    41761 acagatcctt tcttattctg tatcgtatga aaaaagccaa agagaagcgg caaaagagca
    41821 gtagttggct ggctgctgtt ttgccgcagt cgcttttttc tttttagacg tcatacaaag
    41881 tttctgtatg ttcttttact ataacaatct taatcttcta aagatttata ttcaggaacc
    41941 catttagcag attcaacagc ttctttgata tccgtgattt cttcacgatt caatttttgt
    42001 gcaacaactg attttgcgac agtttcagca atcgtttgag agaattcagt gatttttgct
    42061 actggaggca agatagctgc tcctggtttt gttacatcaa cgattccgcc taatgctcga
    42121 ctagcttgag aaatgatttc agcatttact cgtgtagctg ttgaagcaat caaaccaagc
    42181 cctaaacctg ggtacatcaa cgcattattt gcttgaccga tttggtaagt tacaccgtta
    42241 tattccacat cagcagcagg gatacctgtt ccaactaaag cttttccgtc cgtccattca
    42301 atcaaatctt ttgcttttgc ttctgccaat tttgttggat tagacaaagg catgatgatt
    42361 ggacgtgggg tatgagccgc catttctttc acgatttctt cagtgaatgc tccaggttgg
    42421 gtagaagtac cgatcatgat cgttggatgg attgctttta caactgcttc taagtttgtc
    42481 aattcatcag cattagtgaa ttctgagcgt ttacgagcga atgggatttg accaggagtc
    42541 aatccttttg tatcttcaaa caataaaccc tgtttatcta cttgatagaa atgttggcgt
    42601 gcttcttctt caggtacacc ttgacggatc atttcatcta aaaggatgtt tgcgatacct
    42661 acaccagcag taccagcacc aaatgtcaag atcgtttgat ctttcaattg ctcacctgat
    42721 atattcaatc cacctaatac accagctagt acaacgattc ctgtgccttg gatatcatca
    42781 ttgaaagtag tgattttatc ttcgtatttt tctaagatcg ttgcagcatt tcctcgtcca
    42841 aaatcttccc aatgtaataa caattctggg aataattcag tagctgattg aacgaattga
    42901 tcaatgaatt cgtagtaagt ttcgccttct acacgagcat gacgattccc taagtataat
    42961 ggattattca ataattcttc gttattagta ccagcgtcga ttgatacagg caagacttgt
    43021 gcaggattga taccagccgc agcagtgtag accatcaatt taccgatagc gatgtcaaca
    43081 ccgttcacac cccagtcacc catacctaag attccttctg catctgtcac aacgatcaaa
    43141 cgaatgtcgc gtccatctgc tgcgtttttc aaacttgctt tgatattttc tggttcgtcg
    43201 attgaaagaa atgcagcatc ttgtggtttc aagaaaattt cgttgtattg ttcgattgaa
    43261 tccgcaacga caggatcata aacgacaggc atgaattcaa ctaaatgttc gcccatcaat
    43321 ttataaaaca atgtccggtt cgtattgaat agattcatta agaagatacg tttttccaaa
    43381 tcggtctgtt tgcttaagta ttgaccgtaa gcttggacag attgttgttc caatgtttgg
    43441 acagtacttg gaagcatacc agtgattccg tattttgccc gttcttcttt tgtaaaagct
    43501 gttcctttat ttagaaaagg attatttaat aagtttagcc ctgtaagcat gaatgattcc
    43561 tcctaagttt taatacaaac gaagtatagg agtcagatga ggatatagtc aaaaagagca
    43621 ggtataataa attttattta tatgagtaat agagtcaaaa gaaatcaaaa tagcggtatt
    43681 tgttcttttt atatcaacaa tccttgcttg attactatgc gtatctctta tatgctaaag
    43741 aaagaaaagt agaagaataa aggatatgtt agatagataa atagaatctc tgaagaataa
    43801 atcacgtgat cgtgacgata aaatacactt acgtcaggtg ataaaaaata cataagtaga
    43861 aaactaagag gaggaacaga aaatgaaaga agttcaattg atcagaaaaa gcgaactatc
    43921 agaaggtggt tgcaatgcat gcggcgtagt agaagcaaca agctatacat taaagcttgg
    43981 tgccaacaaa gcgataatct ctgagttgac agtaggcggt cttgtggaca gtctggcgct
    44041 tgctgaaggt tttattggag aagatattta tgagatgttt tcagaaatcc gtcagttaaa
    44101 aaaaggggaa aactgcatag aggttcatca tgagagcccg aatgtgagat tcaaacgtgg
    44161 tgataatgag atgatcttca ataatcatgt gtccgatcac accgagctat atggaatcgt
    44221 gaatcaaata ctaactgagc tttttggatt gggaccttat gcgttcaagg aagaaaacgg
    44281 taatccaaaa ttaaacgaag aatggcaaga aacgattgaa atacaaagaa acaacccgca
    44341 cttatttcaa taggcatcca cagtatttcg taaaagggac tttgacacga cttttgtcaa
    44401 agtcccatcg ttttttatct atttgtgttc taaacatttc tttcagagta gtaacaactg
    44461 gaatgactga aatgtaaaca gattatttgc ctgtatcaat acggaagagt aatataaagt
    44521 gtgtaaacgc tatcatatta ttcattcaat tttttataga gggaaggcat tgaaatggaa
    44581 aaaacgtatc aagcaggtac aaatgaagga atagtggatt ttatcaatat ggaagattta
    44641 gaaatagccg cttcacaagt gattccagct ggtggttatg gttacatcag cagtggtgct
    44701 ggcgacttat ttacttatca agaaaatgaa cgggcattca atcatcagct gattattcct
    44761 catgtattaa aagatgtaga attacccgat acaactacac attttgacaa agaaacgctg
    44821 actgcaccaa tcatcatggc accagttgct gctcatggct tagctcatgt gaaggcggag
    44881 aaagcttctg caaaaggtgt agcagatttt gggacgatct atacagcaag ttcttacgct
    44941 tcttgtacat tagaagagat aagagaagca ggcggggaga aagcaccgca atggtttcaa
    45001 ttttatatga gcaaagatga tgggatcaat ttggatattc ttgaagtggc caagcgtaat
    45061 ggagcaaaag caatcgtatt gacagcggat gcaacagttg gcggaaaccg tgaaacagac
    45121 cgcagaaatg ggtttacctt cccactgcca atgccgatcg ttcaggctta tcaatcagga
    45181 gtaggccaaa cgatggatgc tgtgtacaaa tcttccaaac agaagcttag tccaaaagat
    45241 gtagaattta ttgctgctca ttctgatttg ccagtctatg tgaaaggtgt ccagtcagaa
    45301 gaagatgtct atcgttcatt agaatctggt gctggcggaa tctgggtatc taatcatggt
    45361 ggccgtcaac tagatggtgg tccagcagcg tttgattcgt tgcaatatgt ggcagaagca
    45421 gtagataaac gtgtaccaat cgtgtttgac agcggtgtac gacgcggaca gcatgttttc
    45481 aaagcaatcg catcaggtgc agatcttgtt gctattggca gacccgttat ttatggattg
    45541 tctttaggag gaagtacagg agtccatcaa gtgtttgatt tctttaaaac agaattggaa
    45601 atggtcatgc aattagctgg aacacaaaca gtagaagata tcaaaaaaat aaaattgcgt
    45661 gagaatcgtt ttatttaaga aacaaatgac gaaaaaaata ggagtgcggc agatgatgcc
    45721 gtactcctat ttttatagtt caaggatgtc actaatagaa taatttgaaa cgaaaaaata
    45781 aaattgaaat attttttcat acttaataaa aaaggaagaa tattcatgta aacgatttta
    45841 ttgttttttt ctcttttata caaaaagggt cataatttca agtaagcgga tgaattcatg
    45901 aggatttttt gcatactgtc tctactatac ttaggtcact aagaaaaagg agtgatttat
    45961 tatgaaaaag aacatgattg ggacattatt actttcaaca cttcttctag gcggcttggc
    46021 aacaccagct tttgctgagg gtcaagcgac ttcaaaagga gatattacct ttactgaacc
    46081 aacaaatacc gtggagcctc ttaaccctac tgatccttca aaaccagtag agccagcaga
    46141 tccggaaaat ccagcaactg gccagacagg tcctttaaca ctggatgtag ttcccgaatt
    46201 gccatttggt acacacgaga ttgaaagcgg aacaaaaacg tatcaagtag acgcttcaaa
    46261 aaacgataca ccttatctcc aagtatcaga tcgtcgaggt gtaggtgcag atggccaagc
    46321 acaagggtgg aacgtgacag tttcggtttc agattttgta aacggttcac aagttttgca
    46381 aggcgctgaa ttagattttg gaacatccac tgttaaaagt acgtcagata acgaatccac
    46441 tggaccaact agtcaaactg taactgggtt gagtaaagca agtgcagcga caccaatatt
    46501 tacagcagca aaagatcaag gtctgggaac atggctttct gtttatgatc cagcaaatat
    46561 cacactgaaa gtgccgaaag cagcagctgg aacattcacg gctgatttga cttggaacct
    46621 cgtggctggc ccagtagcat agagtatgaa ataaattcaa caaactgcgg tgaaaatact
    46681 ataggtgaag gattttttac cgcagttttt gttgaaagga tgtggtggac aatgaatcaa
    46741 agtaaacgac tgtttgtttc gtttttttct attttaagtc tatttttcat ttttccgtca
    46801 atcagccaag caaaggattc agcaggagac tttggaatca aaccggtatt tccagagaac
    46861 caaatagata aagcaatcgg ttattttgat ttattagtgg caccggaaca aaatcagaca
    46921 ctagaagtaa ttatcagcaa ttcttcagat gaagaacgaa catttgaggt ctctgtcaat
    46981 ccggcggtaa cgagtgatgg tggaacgatc gattatagtc aaaaaaaccc aacgttagat
    47041 gagacattgc cgtttgatgt ccgtgacgtt ttgctcattg cgaaaaagga aatcaatgta
    47101 tcagcacatg cagaaacaac tgtacctata gaagtgaaaa tcccagcaaa aagctttaag
    47161 ggacgtgtat tagcgggtat tcatgtttca cctaaagaag aagctgaaac agaaaatgct
    47221 acagaaggag cacaaatcaa aaatcgtatt gcttataatc tcgcagtcgt tttacaagaa
    47281 agtcaagaga cgatagaacc ggatctgaaa cttttatcag gagatttgga cgaggtcaac
    47341 gcaaagccaa ctgttcagct tcgtttccaa aatcctcagc ctaggattat cagcaatttg
    47401 atatttacga gcaagatctt ttatgaaaat cagctttata ttgaaaatac gtctaatgca
    47461 tttttagttg caccaaattc gaattttcac ttgaatcttg atttagctgg tgataaagca
    47521 aaagccggcg attaccgcgc agaaatcata gcaaaatcag gcgacagtaa tgaatggcga
    47581 tttacacaaa actttacgat caaaaaagaa aaagcacaaa aagtaaatga aaattccgtt
    47641 tttgcagtac aagaacaatc tttcccatgg ctttatgcag gattggttgc tgcagttcta
    47701 gtgttatggt tgattttgct gtttttgtgg gtccacaaga aaaagaaaaa aggagaaaca
    47761 acatgatgag gtgatttcgt tgaaaacatc gatcttattg ggagggatta ttgcaagttt
    47821 gctgttcttc agctggcaag agccagtcca agcagatacc aaaagttcag gtacgattac
    47881 attttatgat ctaagtccaa ttccgcatcc aagtggttca gtcaatggcg catcaaaaca
    47941 gcagacacag ctaccgattt ataaagaatc taatcaacat ggcgtattcc cggagacgaa
    48001 tgaacaaaag atccgttttc tttatttgat cattggtata gaattatggc ttcttgcgtg
    48061 gttgttgtta tggagaagaa aggaaaagga ggatgaacat gttgctacaa aataaatttt
    48121 cattttttcg ctccttcttt tttacttcgc tttttgtttt aagtatattt atcttttcac
    48181 cttcggcaga agctgttgga gcaacgcctc ctgccatttc ttatgatcca tacacgtatg
    48241 aaaatatcgt acctccaaat ggtggacgta gtgcagtcaa tggcgggaag cttgtccaac
    48301 taactggggc taaccaattg cctaaattga ctgctactta tttgaacgat ggttcaaatg
    48361 atccttggaa aggtggcacg gtccgttttc attgggaaag ccatgtgaaa ctaacaaatg
    48421 tacttgattt ggtgaatatc actgcacaag gatttgcatt tgtggcgatc atcaatttgc
    48481 cggaacatgt atcaagcagt gatgtattaa aagcaattga ctggaacaac gcctacttgt
    48541 atattgccgg tgcccccatc aaggttacta gcggaagttt attatcagca ggaagccata
    48601 ccttgcgttt tgggatgggg tcgtgggata caaatactct attgacagat attattgcta
    48661 ttattacgcg aggcgaattc aacgtggacg atatcccgct tcaatttgat gttacagcta
    48721 atgtcgcgaa gatgacagaa aacggagata aatatgatac gagtccaaac aaaattttga
    48781 ccaagggtaa attacaacct tctatgtcta aaaaggccgt tttttctgtt gatttttatg
    48841 attcgaataa tattatccaa gatcgtatgt tcaattcgat tttagctcca gctgcaggta
    48901 gattttatcc taaattaact ggcggaaact tgaatccagg agcatatgca ttttctgcaa
    48961 gtgcagagat aaatacgtgg aatagttaca tctcaccaag tgattcaaca ggaaaatata
    49021 atacatttgc tgataccaca gaatacgtgg atggaaataa ccagaacctt ccaggctcta
    49081 ccaatagtcg gacattgacg atggggctga ctgaaggacg ttttgatcag ttgccattta
    49141 gtcgctttga tcgcgtagtc aactatttta caggaaaaaa cgttacacaa ggagcaatac
    49201 tgactcatac tccagcaagc acaagtggat tgaatcagac agccaatatc gtgtacagtg
    49261 ggaaagatgc gcaaggcgga ttgctttctc ctgttgcttt aaaggtattg gagcagtaca
    49321 agctagacgg aacggtttta ccaacaaact taacagccga tagtcagtgg ggaaagctcc
    49381 tttcttctgc atcgtaccaa gtcacggcca attggtcagc aactgaacta aaaaagggtt
    49441 cgatccatta caggctttat tcgaaaggaa cccaaaaatt agcgacagga tggacagatc
    49501 aacttttcca aacgatccaa tcaaataacg ggggtgtaaa tacagctact tctacactac
    49561 ctgcattggc ttcagggcaa tattattttg attatcgctt ggtggacgat gtacttacta
    49621 acatgtatcc gtcttttgcg tacaaatggc agtctgaaca agctggaatc accactcttc
    49681 ctcaaataac agtggctaat tttccatctg tgagtgcaga tagtcagcta aaaaacctta
    49741 gcagacaacc tgagacagga gatcccctgt ctgctctatc aggagatacg attcaagaaa
    49801 atgtgacatt cacgttaaca aaatctgggg atgcgataac cgacaaaaaa atcacgatct
    49861 cacttcctga aaatactact tatatcaatg gttccttaaa attaaatggt acaactatcg
    49921 cagataccgg cattcaacaa ggagtttctg tcccagcaga tctattgagc aaaataggag
    49981 ataccattca tcttacttac aactatcaat taaatactgt agatacttct ttacaatccg
    50041 tcagcatact gacaaaggca gctgtacttt ccagcaatat tacattagca gatggtgcta
    50101 agcttcctaa cccagttgtc caaacatctg ctaaaacaat tcttgtcccc aaacaagaat
    50161 taacattagt taatgtacca gatgatttta catttggtaa cgatctcccc aaaccgttga
    50221 agacaagtta ttatgaatca aaaggcgatt tttcttttga tgtgcgggat actcggcttc
    50281 caagcacgtc tccttggcaa ctgacaggta cattgacttc tttatttaag aatgatcaag
    50341 gtcaagaatt aagcggaaca aaattatact tcaatcattc tggatcaaag caattgattc
    50401 agcaaggtca aaacacgttg atttatgaga gtgatggaac agctaaagga gaggttctcg
    50461 ttgactttcc cgataccgat ggtttactct tagaggtaaa tagcagtacg aatgctcaac
    50521 ctggcgcaac gtatcaaggc atggtcacat ggaaactgac agctgggcca accagctaag
    50581 tgattagaat ttgtatggat ctgtgataac tgtcacagat cctctttttc aaggatgaga
    50641 aggaaacttc tgctgttaaa atcagaaatg cttgaaggag tggacttgga acttttcagt
    50701 ttgcttgaaa aaaatgaaca acggcaaatc aaacttttcc aacttttatt agaggaaaaa
    50761 ggagaggttt ccgttaataa cgtacttacc cgcttatcag ttgatcgggc taccttgaaa
    50821 gaagatctgt tttatttaca agctaacttg gaaaatatca aacaattgga aattgttatc
    50881 caagaaggga atattttatt aatcagacat ggaaatatag cagcagtcga tgtttattat
    50941 cattgttact tgcgctattc tacaaaatat cagatacttc tttatctgtt ggaaaatggc
    51001 tcatttgatc ggcagaaact tgtcatggaa cttaatatca gttcggctac tttgtctcgc
    51061 agagtgaagg aattaaatga ttctttaaat gagtttcatc tgaaaataaa aagcgggcgg
    51121 ctgaccggtc cagaaagtca aattcgttat ttttatttcc aattattttg gtttggacgt
    51181 ccctatcttg aaaacaaaaa agaatacagt gatttatcag gaaacacttt tttagatctg
    51241 ctgcaaaaag agtttagttt tgctattaca gaagaaggac gattgaaatt tgaaatatgg
    51301 ctttccatta tgaagaagcg aatgcgctgt acgaaaaaat ccaaaccaga ttatagcaat
    51361 cttcctgttg gattgtttca aacgacacaa tttttattgg acctccatac atttttgacc
    51421 cgttacttct ttcaatatgc ctttccttgg aatcaaaatg aaacgttggt tttttatctt
    51481 ttcatgatca gtaactttac attagataca cataactcgc atgtccagga gtttttgata
    51541 agcgaaaaat acaaagacga cgtagtagct caattgaatc gaacgtttaa acaatcgata
    51601 gaagaatatt tctcttttta tgtttttcca gaagagattt cgcaaaagat tgcgttgaca
    51661 gtgatgcaaa atcacgctca ttttgcctat tttagaggat ggatgaatat ttttggccaa
    51721 gaaggattaa aacagcggtt aactgaagta gaagataaac aattcctggt tttatgtgaa
    51781 agattggtca gacaaacatt tgctattgtt gattttactg aagtagacga ggaaaatgcc
    51841 cgctttgaat tatttgctcg ctatgcatct attttacgga cgctgatcaa gcagttagaa
    51901 ttacgtctgc aaattgctta tgatcttcct tatgaaaggc taatggcgga tatcgtgaaa
    51961 gaaatcatcc aggaacaatt gctgaatacg atcaaatatg atttagtcga atacgagaaa
    52021 gaaaagcagt atgatatcat tttgactagc caattgaaag agtacccgtc acagaagtcc
    52081 gctaaagttt ttgtattcac tagcaacgaa tataattatg atttccccta tttgaaccaa
    52141 tttttaaaag aatgttattt agaaaaattg aatttaagga tgaataaaac atgaaaatcg
    52201 tgatcattgg tgcttcccat gcaggcatca cagctgccct aaatctaaga aaacttcaac
    52261 cgaaagcaga agttttactg atcgatgaag atcataaaga tggactaggc tatgtttcaa
    52321 atggaatcaa cctttattta aaaggaaaga tacgtagttt agctgaagtt gcccataaca
    52381 tgcggactct tcaagaaagt ggggcaaaac tgatcaccga atggagagta actgaacttg
    52441 atccagacaa acatcagctc atcctcgcat ccaaagaagg aaaaaacgag accataacct
    52501 atgataaatt aatcgtagcg acaggttcct ctcctgtcac cttgtataaa caaattgaag
    52561 ctgaaaatgt ctacacctat aaaaatctag tgcaatctaa gcaagtactt gccgctttaa
    52621 aagaagcaaa agaagtagtg atcttcggtg ctggttacat tggattggaa ttagcagatg
    52681 ctttgagaaa taaaggatac attattcatc tagttgatta tatgccgaat gttttatcaa
    52741 gatatttcga taaggatatg atcaacagtt ttcaaaatca gctgcaaact aagcaaataa
    52801 atttttatcc aaatgaattc ttgatcgatt ggaaaaaatc agaagagaaa gtagtatccg
    52861 tacaacttct gtctcaaaca ataaaggcag atatggtcat tttttctgcc caaactcgtc
    52921 ctaatacgac attgttaaaa gaaaaagtgg ctttgtatga ggatgagact gtgatagtca
    52981 atgaatatct gcagacaagt gatccggaca tctatgcgat tggagatatc gtcccagtca
    53041 gttttgacaa aaataaacgg catctctttt tacctttagt tactcgagct gtacatatgg
    53101 caagagcagt agcgctaact ttgagtggtc agccaaccgc ttacgacctc agacaaaaga
    53161 taacagcagc agtcattata gactactttt taggaactgt agggctgaca gaagatgaag
    53221 cgccattctt ggaacaaaat accggttcgt gttcaggaga atttgatcta tttccacaat
    53281 acgacgaaga aaacaaaaca gtaaatgcaa agctgatcta ccatccggat acattagaaa
    53341 taattggcgg acagctgata agccaagaat tcttattatc agatttgaac ctgcttgcgg
    53401 atatcgtgaa acacaaaact acgattccac agctagcagt tgaggatttt ggatttctgt
    53461 ctgaatacac ctctcgtttt cactacctca atgagttagc ttttaaagta ctagctaaga
    53521 gagcaaatag caatagagta gataaaagac tgtgagaaat atcacggtct tttattgtgt
    53581 ggggatataa taaatattaa tcaatcgaga ataaaaaatt catataatga aatagcggaa
    53641 gaattttttg agcataatat tcgccaaaaa tttatcaagt gataaaattg ttgtataaaa
    53701 tgattatttc aggaggagga gttaaatgtt tttagcatta aatgaaatta tgcattcaaa
    53761 gttacgttat gcgttagttg caggggtgat gtttttgatc gcctacctgg tatttttttt
    53821 gactgggtta gcttatggct tagctcaaga taatcgaaca gcagtcgaca agtgggaggc
    53881 agattcgatt gtgctttcga aagatgcaaa ttcaaatcta gggatgtcca tgattactaa
    53941 gaagatagca gaagaagtcg aaggtgggaa agtggcgtat cttgcccaaa cacctggagt
    54001 cgtgactagt aaagacagta cagaagaagg aaaaatcaat gttagcttct tcggtatcga
    54061 taaaaatcaa ttcatcatgc ccaatcttgt tgaaggaaaa gcttttgata acgatgatga
    54121 ggcagtaggc gatattagtt tgaaagaaga atatggtcta gccataggag atacagttaa
    54181 gttatctggt agtgacaaga cgtttaaact aactggcttt acggatcatg ccaaattcaa
    54241 tgtctcacca gttctctata caaccatcaa tgcttaccaa gaaatccgct tcgaaaaaga
    54301 agatacaagt gaaaatgcac ggattaacgc aatagttgtc agaggtaaaa tcagtaattt
    54361 acctgaagat ttagaacaga tcaaaatttc taaattcatc aatgaattac ctggatataa
    54421 tgcgcaagta ttgacttttg gatttatgat cggatttttg atcgttattg cggctattgt
    54481 gatcgggatt ttcatttatg tactgacgat gcaaaagatt aatatctttg gtgtaatgaa
    54541 agcacaaggg atcacaggag gatttattgc gcgttctgta gtggcacaaa cattcatttt
    54601 atcatttgtt ggtattcttc ttggtctatt aggaacagtg ggaacatccc ttgttctacc
    54661 tgatgcggtt ccattccaaa gcaattggct gttttttggc gtcattagct tgttgatgtt
    54721 agtagtagct gtgttaggcg cactattttc tgttcggaca atcgtaaaaa tcgatccgtt
    54781 gaaggcaatt ggataagaag gaggaagaac atgaaagcaa tagaatttaa agaagtagaa
    54841 aaaagtttcc aagatggcga ccaagtagtt aaagcattga aagaaacaac catgagtatc
    54901 gataaagggg aatttgttgc aatcatcggt ccttctggtt caggtaaaag tacttttttg
    54961 actttggcag gcggattgca gacgcctagc agtggctctg tatttatcaa tgatcaagaa
    55021 ttcagtaatc aaaaagaaag cgaacgcgtt aaactacgtt ttaaagaaat tggctttatt
    55081 ttgcaagctt ccaatctggt tccttttctg acagtaaaaa agcagttatt attagtagat
    55141 aagattaaac atgaacatcg tcaaaaagaa gcaaacgaat tatttgagca gcttggcgtg
    55201 gataagttga tcaacaagta tccagaagaa ctatctggtg gagaacggca aagggtcgca
    55261 atcgctcgtg cattgtacaa tgatccgtcc attattctag cagatgaacc aactgctagt
    55321 ttggattcag aaaaggcacg agaagtagtc gatatcttgg caaaagaatc aaaagacaaa
    55381 aataaagcaa cgatcatggt tactcacgat acacggctga ttgacaaatg cgatagaata
    55441 tttatgatgg aagatggtgt attgaaagaa aaagaataac caaaaagcag gtggaaaaat
    55501 ggtccatcga tacaacaaag atacacagca aaaaattttg aaagaagcga ctgacttgtt
    55561 tatgtccaag ggatatctcg gcacttcaac aagagaaatc gcacaaaaag ttgggatcac
    55621 ccagcccaat ctctatcatt atttcggtga taaagaaaaa ctatatacgg cagttttaga
    55681 aaaccactta aaagatgttg gaaaagcttt gagagaaatc gttcaaacaa gcgaaacggg
    55741 atttcaagta accttgacaa aaatggcgca gtttttgatt gagacacatc tggtcgattt
    55801 gtttctgatg cttcacgatc ttgaaagcaa tctttcaaag gaaacaagag atcatctttt
    55861 ctttctatgg aagaaaaatt atcgtgaacc ttttgaagaa atcttttcag caaaccaatc
    55921 tgtgctaaga aatgggattt caaaagaaat cgcagcaagg cattttttct tagtgttagc
    55981 accttacatc actcggtcga aaagacctgt ggaagaaccg ctaaatgtgg cgcaattgat
    56041 cgatttatat ttgaatggtg tattaggaga agcgtaaagc tgttatatag ggagaaaata
    56101 gttaggtgaa gaagcaagat atgacgaaga tcgttatatc ttgcttcttt atataggtag
    56161 tttttcataa agacaaattt gttattctct catgattatc agggtatatt tttatttatt
    56221 atgtcggtgt tccaggaagt gttttaattt aataacgaag aaattaattg tttgtagaat
    56281 gaaggaaact atttattggg tagatgaatc accacgaata gtcaaaagga gatttcagga
    56341 tatgccaaaa atgaaaaaaa tcattttagc agtaatgtta ttttctacgg ttatatcttt
    56401 tacagcccct tttttgctta cttaagctga ttttaagttt tggcccttag tctggatcta
    56461 cgaggtgaga gtcaagaatg aaaaaaataa ttggagcttt tgtatttgca atcattatca
    56521 ttattatctg ttttcaaata gatgtttttg gtcaaaaaaa gatagttgga caaacggtca
    56581 gcagtacgaa cagtacggtt acaaagacaa aggaatcatt tgatgataca gaacaagaga
    56641 cattaatcaa aatcggaaaa aatgaccgta cactatatac agatgactcg ctaactaccc
    56701 cacttgcaac tataaacggc ggagagctgg cggagtttct ttctgaaaca aaaaatgctt
    56761 atcagatcaa gacaaatgat ggctatactg gatatttagc cctcgaagat ggaaccaaag
    56821 tcacaaagaa tatacaaaca aaaccggaag agctatctga tgcagtgatt gtattagatc
    56881 ctgggcatgg cggaaatgat actggggcat taagcaatga tgaacaaaca gaagaaaaag
    56941 atataacatt aagtacagcc ataaaagtaa aaaaggcgct agaggatgct ggagcaacgg
    57001 tttatttgac ccatactaca gatgaattag tacagcttgg agatatatgc gactacagtg
    57061 aagaaaaaaa ggcagatgtc tttatcagtt tacacgcaga ctctacggaa tatgcgaatg
    57121 aagcaactgg aatcacgaca tattattact atggacaaga ggaaacatta gcacagacaa
    57181 ttgctgactc cttcactgat ctgcctctgg attcaagagg aatctccact gggaactatc
    57241 aagttcttcg agaaaatctt caaccttcta ttttaatcga gatgggttat atgaataatg
    57301 atagtgattt aaaggaatta gtaacagaca gttatcagca gcagattgct gcaagtttga
    57361 cacaagcatt gaccacttat tttcagtaat aaataagccc agccaaacaa aaatagctcc
    57421 tcatattttt cgtttggccg ggcttattat cgcagatgca agcttttgaa caccgtttat
    57481 ttttttagta gcttcaaagc tttttctgta tttgcaaagt gaagtttggc atatttttca
    57541 agcataggca tgccgccttt ctccaatagt tttgccgcta cttggtcaga tttcgttcca
    57601 gctcctgatg gaagggtgat tccagcttca gctgatgctt tgtgtaactc ttccaaaaag
    57661 ctggcacgac tgatgataga agcagcagca acagctaaat gatattgttc tcctttagta
    57721 acgaagtaca gtttttcttg taattgattt ttttctaaac gcgcatattt tttaaaattt
    57781 gcttccggcg taaactgatc gatcaaaatc gcttctggtt tttctggctc gatttttttt
    57841 agcagcaaat ggatcgcttg attgtgcaaa gcgactttca tccgaacggc attgtatttt
    57901 ggttggatct cattgtattt ttctggagat aagacaagca gtttataggg gatggttttt
    57961 tttaagatgt cagcaagctg gatgatttgg ctatctttta actcttttga atctctaaca
    58021 ccaagtttac gtaattctgc aagctgatca cgtttcacat aagcagcaca tactgttagc
    58081 ggaccgaaat agctgccatt tccgacctca tcactaccta aaacggataa ttgactgata
    58141 ttttttggca aagaagaggc tgtttttgct ggcttggctt gtttatcctg tgcctccccc
    58201 catttatcag cttctattgc tgcattcgta ccttgaaaca tcacttttcc ggatgtataa
    58261 gcggtaatgg tcgtcccgtc tttttttgca gcaaagctag tataaggaat ctgtttattt
    58321 aacaaataag agcgatagta tgctttcatt tgactaagtt ggtcgtttgt tgctttgata
    58381 atgacgttgc tcataaaatt ctcctcgttt ttatacataa aactataccg caactcacga
    58441 cttttcgttt tctaagagat tgcgagacat agcatttttt taaagtttat catataatag
    58501 ttaagtatgt gaaaaatatt tgtggagaac ggtgaaagaa ccaacttcat ggtacactaa
    58561 ataaaacttt catcaaatag gaggaaatga ttcgtatggc gcacgaaaaa acacgctaca
    58621 aagcggtgat tgccaaccaa acatatacta ttatcggacg ggaaaccaag catcatatgg
    58681 atatcgtaac gaagctgatc aatgaacagt tagcagaatt gaaacaatta tcaccacaaa
    58741 tggataatga acaagcagca atcttgatgg ctgtcaatgc gctttcggat caattgaaga
    58801 aacaagaaag aatcttggag ctggaagaag aaacagcaga attaaagaaa aaaatgatca
    58861 aatttacaga gcttgaaaat cgtgtgaaac gcattgaagc aatcgagaac gaagctcggg
    58921 aagtattgaa agaaaatggt caagcagatc ataccattaa aaatcatgtg gaagcacaac
    58981 agattttaaa cgagaaacga aaaggacaaa ttaaacaaaa atcttctaat taagatcctt
    59041 ttatcaaaat aagaaaaatt taaaaaaagt tagaagagaa aggaacgtag catgctgagt
    59101 ttgctgattt tatttttatt gctaattgcg tttttttctg gagcacgtcg cgggtttgct
    59161 ttgcaaatca tattcgcgat tggatatgta ttgtcattta ttgctgccca gcatttttac
    59221 aaaccattag caagtcattt ggaattatac attccatacc cagctgtcac gccaacatca
    59281 aagttagcat tttttgatca ggtatttgcc tttcacctag atgaagcttt ttatgcagga
    59341 actgcttttc tgattatttt gctaattggc tggctgctta cccgatttgt gggggttttt
    59401 gtccatggat tgacgtatgt gccgatcttg agacaggtag actggattgc aggaggcatc
    59461 ctgtcactaa tcatggctta cgttactata tttttgattt tgcagttact tgcctttgtt
    59521 ccattagatg tggtccaaaa tcaattcggc gagcatagtt tcgctcgctt tatcgttgag
    59581 agaacgccat ttcttaccaa caagatgtat gagctttggg taacgaatat tttgaattaa
    59641 ggaaaatagt gctggaaaat atttcgtttt ttgtacagag tagattattt aagaaggctg
    59701 cgtagcgtat gtccggcaca gctctttttt catagtgaaa gataaggtga taaaatgaat
    59761 aaccgaatcc ttgaaacatt ggaatttgaa aaagtcaaac agatggtcag acaatttgta
    59821 gtgactgcac aaggaaaaga agaactggca gaattggtac cagtgagcga ggaacaaacg
    59881 atcacaaact ggcttcaaga aactgaggat ggattgaaag tgcaacgtct gcgaggcggg
    59941 atccctatac ctaaacttga aaatatccgt ccccatatga aacggattga gatcggagca
    60001 gacttgaatg gagtggagct tgcacaagtt tccagagtat tgtctacgac aagcgaattg
    60061 aatcgtttca ttgatgatct ttatgacagt gaaatcgaat ttgcacgttt gtatatgtgg
    60121 gcagatcagt tagtcacgat ccctgttttg agccgccgtt taaaggaagc aatcgatgaa
    60181 gacggacgcg tgactgatga cgcttctcct gagttaaaga gcataagaca gaacattcgg
    60241 cgaagtgaac aagctgttcg tgaacagtta gatgggatcg tccgtgggaa aaatgcaaag
    60301 tatttaagtg atgcgatcat caccatgcga aatgaccgct acgttatccc agtcaaacaa
    60361 gaataccgcg gtgtatttgg cggtgtagtc cacgatcaaa gtgcttcagg acagacttta
    60421 tttattgaac caaaacaagt ggtggatttg aataatcgtt tgcgtcaata ccaaatcgct
    60481 gaacgaaatg agattcaacg tattttaagt gaactttctg ctgaacttgt tccccatcgt
    60541 caagaaatca tccacaatgc ttatgtgatt ggtaaaatgg atctgatgaa tgcaaaagca
    60601 cgatttggta aagaagtaaa agctatcgtg ccgggaatca gtgaagacaa tcatgtcgtc
    60661 ttgaaacaag caagacatcc actgatcgat caggaaaaag tagtttctaa tgacatcaca
    60721 atcggaaaag actatcaagc catcgtcatt acaggaccta acacaggtgg gaaaacgatc
    60781 acgttgaaga ctttaggtct gttacaactg atggggcaag caggattacc gattcctgca
    60841 ggtgaagaaa gtcaaatcgg tatttttgag gaagtatttg cggatatagg agacgaacag
    60901 tcgattgagc aaagtctatc gaccttttct tctcatatga cgaacactgt agatatttta
    60961 tcaaaagtca atgaaaaaag tctggtatta tttgacgagc tcggtgcagg aacagatccg
    61021 caagaaggtg ctgctctagc aatcgcaatc ttagatgatc ttggtaaaaa gtctgcttat
    61081 gtgatggcaa caacgcatta tcctgaattg aaagtttatg gatataatcg ggcaaatacg
    61141 attaatgcca gcatggagtt tgatgtggat acattaagtc caacgtatcg tttgctgatt
    61201 ggcgttcctg gacgaagcaa tgcgtttgaa atctctagcc gattaggtct tgatacagaa
    61261 gttattgatg aagcgaagca gttgatgaat gatgagagcc aagatttaaa cgaaatgatc
    61321 acagatttag agaatcgccg gaaaatggca gagaccgaat atctggaaat gcgtcatttt
    61381 gtttctgagg cacaagaact tcacgatgat ttgaaagaag cttacagcta tttctttgaa
    61441 gaacgtgaaa aagaaatgga aaaagcaaag aaaaaagcaa atgaagtcgt gtcagaggcg
    61501 gaagaaaaag cagaaaaaat cattgctgat attcgtaaaa tgcaacagca gattgggcaa
    61561 ggaaatgtga aagagcatca gttgatcgat gcaaagaccc aattagcaaa tctacatcaa
    61621 gaagaaactt tgaagaaaaa taaagtattg aagaaggcga aagaacaaaa aacgttgaaa
    61681 ccgggagacg aagtgttggt tacgacttac ggccaaagag ggacgctttt acgtaaaaac
    61741 gggaatcagt ggcaagtaga aatcggtata ttaaaaatga atgtgtccga agacgagctg
    61801 acgcctgttg ctcctcaaaa agagccgaca caacgagtta tccatgctgt acgatctgaa
    61861 tctagtagtc acgtgccgaa tcaattggac cttcgaggaa aacgatacga agaagcgcta
    61921 agtgaggtag atcaatattt ggattctgcc attctagctg gttatcctca agtgactatt
    61981 gtccacggaa aaggaacagg cgcacttcgc aagggaatta ctgattatct taaaaaccat
    62041 cggagcgtca aaagctttga gtttgcaccg gcaaaccaag gcggaaatgg tgcgacaatt
    62101 gttaaattta agtaaagctt actaaaggta agcagatggt tcgctatcca agtatagaaa
    62161 tgctatacta gtcatagatt ttaaaaatgg aggtctttga tatgtcacaa gtaattacag
    62221 ataagacatt taatgaagaa acagataaag gacttgtatt gattgatttt tgggcaactt
    62281 ggtgtggtcc atgtcgtatg caagcaccga ttttggatca gttggaacaa gaatatgatg
    62341 aagaagaatt cagaattgct aaaatggatg tggatgaaaa tccagaaact cctcaacaat
    62401 tcggtatcat gagcattcca acattaatgt tgaaaaaaga tggtcaagta gtagaaaaag
    62461 ctgttggtgt tcattcaaaa gaacaactac gtcaaatgat cgatcaatat ttataaaaac
    62521 ttacagcaaa aataagctga gggtgggact gaatccagtt ccgtcctcgg tttttttgcg
    62581 gttgagttaa ggttattcaa attaatattt tttgagtata attacgttgt caggctggat
    62641 tgtttttttg acagacatgc tataataact ttaattgaat atgaagggga gtaccagatg
    62701 aacgaacgaa tcaaaaacaa attagcttta cttcctgatc agcccggctg ttacttgatg
    62761 aaagataaaa atggaacgat catttatgtc ggcaaagcca aaatattaaa aaatcgtgtc
    62821 cgctcatatt ttactggtag ccataatacg aaaacggaac ggcttgttag tgaaatcgaa
    62881 gactttgaat acatcgttac agaatcgaat atcgaagcat tgctcttaga gatcaatctg
    62941 ataaagaaaa atgatcctaa atacaacatt atgttaaagg atgataaaac ctatcctttc
    63001 cttaagataa cgaatgaaaa atatccccgt ttggtgatta ctcgtaaggt actaaaagac
    63061 aaagcactgt attttggacc atatcccgat gtcggtgcgg cgaatgagac aaagaagatc
    63121 ctcgatcgat tatttcctct gagaaaatgc aaacctagtc agacgaagga gccttgtctt
    63181 tactatcatt tgggacaatg tctgtgtcct tattatttcg acgtagatcc gaaagtctat
    63241 acgaaaatcg ttgatgaagt gaaaaacttt ttaaacggag gacacgaaaa aatcgagtca
    63301 gagatccatc agaaaatgga aaaagctgct gaaaacatgg agtttgaaaa agctgcagaa
    63361 taccgagagc agatccgtgc cattgagaca atcatgactc gccaaaaaat gacaaatact
    63421 gatttactgg atcgagatgt atttggttat gcgattgata aaggatggat gtgtgtccaa
    63481 gtgtttttcg taagacaagg aaagctaatt gaacgagatg tttcaatgtt tcctttctat
    63541 aacgaagcag aagaagattt cttgacttac ctcggtcagt tttatcagga aaatgagcat
    63601 ttcattccta aagaagtctt gattcctgat acgatcgaca agcccagtgt agaagctttg
    63661 ttagctacga aagtgctgca accaaaacga ggagaaaaga aaaaattagt tgaattggcg
    63721 agtaagaatg cctcagttgc tttgaatgaa cggtttgatc tgattgccag aaaacaggaa
    63781 cggacgatcg gagcagttga acgtttagga aacgcgatga atatcccgac cccaatacgt
    63841 atcgagtctt ttgataactc taatatcatg gggacgaatc ctgtggctgc gatggtcgta
    63901 ttcattgatg gaaaaccggc aagaaaagaa tatcggaaat ataaaataaa aacagtcaac
    63961 ggaccggatg attatgcatc aatgagagaa gtcatctatc gcagatattc gcgagtgatt
    64021 cgcgaagggt tgcctttacc tgatttgatc ttgatcgatg gaggaaaagg acaagtagat
    64081 gtggcaaaag atgtcttaga aaatcaatta ggaatcgata ttcctattgc tggcatggca
    64141 aaaaacgata aacataaaac aaatgagttg ctattcggta cagatttggc tgtagtaccg
    64201 cttgagcgaa attcaccaga gtttttctta ctccagcgaa tccaagatga agtccatcga
    64261 ttcgcaatca ctttccatcg gcagctacgc agtaaaaaca gttttgcttc ccgattagat
    64321 gaaattgaag ggttaggacc aaaacgaaaa aaaatgctgc ttaaagagtt caaatcgttg
    64381 aaaaatatca cagcagccac gacagaagaa ttacaagaaa tcggactgcc gaaaaatgtc
    64441 gcacaaaacg tatttgataa attacatcag gaatgaggaa agggcacttt acaaagaaag
    64501 taaagtgtcc tatcatttta tgtacaacaa aaagagcgtt tacgttttgt tggtaaataa
    64561 taaatcgctt acgtctggtg tctaaggata tatgtgattt cttgaagaag gagtgaagaa
    64621 atgttagaag tcaaggatct tgttaaaact tttgggaact acacagctgt cgatcatgtc
    64681 tctttccaaa tccccgatgg aaaaatcatg gggctaattg gccaaaacgg ggctgggaag
    64741 acaacgactt ttcgtttgat cttggatttt ttgacacaag accaaggaga agtgttatgg
    64801 aatggccatc atcttgggga gaaagattat gatgtcatcg gttatcttcc ggaggaacgg
    64861 ggactttatc cgaaagtaac gattcaagac caactgcttt attttgcaga acttagaggg
    64921 aaatcaagaa aagagattga accaaaaatc gacttttgga tggaaaaatt tcaggtaaaa
    64981 gggaaaaaga cggataaagt aaaatctttg tctaaaggaa atcagcaaaa agtccagctg
    65041 atcgccacgt taatccatga acctaaattg atcattttgg atgagccatt cagcgggtta
    65101 gatccggtaa atgctgaatt gctaaaagat ggaatattgg aattgaaaaa acatggttca
    65161 tgtgtcattt tttctagcca caatatggat aatgtagaaa aaatctgcga tcatttgatc
    65221 atgttgcgaa acggaaaaat ggttttgaat ggaaaagtac atgagattcg tgaatcattt
    65281 ggccgtacga agttattctt ggaatctggt ttaagccaac aggaagtaga agaaatcgaa
    65341 ggcgtgaaaa aagtgatcct tcatgaagac aatacgttag agattacctt agaagatcct
    65401 gaagctggga aagaaatctt tactcgtgcg actcagttcg gctacattcc tatgtttaac
    65461 cagcaaccgc caactttgga agagattttc aaattgaaag caggtgaaat cgatgcgtaa
    65521 gttttggatt attaccaaag atgtctattt aaaaaatgtt aagtcgattt ctttcttgat
    65581 catgatatta gtccctttta tcatgctggg tatcatttat gtagcaggaa actttgctca
    65641 acaaaatagt gatgtcaata aaatcggcgt attggctgat aatgagcaaa tcagtcagca
    65701 aatgagccaa gtcaaaacag atgattttga gttcaaagca gttgattctg aagaaaaagc
    65761 acaaaaagag ctttcggaag aaaaaatcga tgcgtattta gtagtctcaa ctgagaatga
    65821 taagataaat gggatgcttt atagcgaaaa ttcattagga caaagtacac aactactgat
    65881 acaacaacaa ctgactggct tgcaatcgat gcttcgagcg agtcaattag gcatttcacc
    65941 agaagaagta gctagcttga gccaacaagc aagtttgact cgacaaaaag tcaattttga
    66001 ttcaaaagga aaaatgacag tcggagaaga taactcgaca gtccaatacg tcgtaagtta
    66061 tgtagcaacg attgtcttgt tcattattat tttgacgtat gcgcagatca ttgcacaaga
    66121 gatcgcttct gaaaaaggca cgcggatcat ggaagtgatc ctgtccagta cgactgcaca
    66181 aaagcatttt tacggaaaat taacaggtgt tttacttgtt gcattaacgc aaatggcatt
    66241 atacggtatt atttttggta taggatacag tcaattcaaa gatatggaaa tggtcaaaaa
    66301 tatattagac ggtatctcat tggataactt gattggacca ttcttgtggt attctcttct
    66361 attcatgatc tttggcattt tgattttttc tgttttagca gctttatgtg gctctttggt
    66421 caacaaagca gaagatacag caaaagcaat tttgccagtg acttatctat ctctaggcgg
    66481 atacatgctt ggattgatac taggagcttc agatccaaac aatatcgtta ttcggatcac
    66541 ttcgtatatt ccattcttat cttcatatat tatgccggtt cgcttagcaa atgagacagt
    66601 cggtatgggt ggagcattga tttccttgtt ggtacttgta gccgtaacat tcttattgat
    66661 gatcttgtct gcaaacatgt acaaatcgaa tgtactcgtt tatagcgaag gcggattatg
    66721 gagttcattg aaacaatcga tttcaattat gaggaatgaa cgaaaaaaag cataaaacaa
    66781 tcctaaaata cagaaggaag ctggacatct attgttccca gcttcttttt gtatagacct
    66841 attttattta ttttactata ttggatatat attcattgaa aatagtgtat aacaacttta
    66901 taatattgag tataccaatt tttggaggcg ttttaatgga ccgttattta gatttacttt
    66961 cagaaaaatt cccctcaacg gaggatgtca tcacggaaat catcaatctc gaagcaattt
    67021 tgcagcttcc aaaaggaaca gagttatttt tgagtgatat acatggcgaa tttcctgctt
    67081 ttgatcatat tctgcgtatc ggttcaggaa atcttaaaga aaagatcaaa gacctttttt
    67141 ctgaacaaat gacggaaacg gatatcagcc aattcactat ttttacggct tatccagaat
    67201 atgcattaac gactgattgg tacaaagaac aagataaatc gcagctgatc catcatttga
    67261 tcgatctttt acggtttacg acagtcaaat atacccggtc aaaagttcga aaaggattgc
    67321 cgaaagaata tagctatatc attgaggaac tcttgtatat cgatgaccgt atcaatggaa
    67381 aaaaagacta tgtaaaaaaa atcattgaac aacttgtttt gatgaaggaa gaagaaaaat
    67441 ttttaacgaa acttgcacag acgatccaaa aatctgtcat cgatcatttg catatcgttg
    67501 gagatatttt tgaccgagga actcaagcag ccaaagtgat ggatcgaatc atggattttt
    67561 cttctgtaga tatccaatgg ggaaatcatg atattttgtg gattggtgcg tattgcggct
    67621 ctgaggcatg tttactgaca cttttacgga ttgcagcacg ctataattat ttatttgaat
    67681 tagagaaaga atatgggttg aatttacgtc cattgttttc ttttgctcat caaacttatc
    67741 agaagaatcc agtatttgcg cctaaaaaca gtaaaaatct atccgaaaga gagcaagaag
    67801 aattagaaaa aatccatcag gcactttcta tccttcaatt caaatcagaa catcagttat
    67861 tggatcgtcg acctgaattt aaaatggacg atcgtaaatt gctggaaaaa atcgactatg
    67921 aagcatcaac gattgattta gaaggtcagc tctatgagtt gaaaggaact tgttttcaaa
    67981 cgatccagcc agatgatcca aaaaaattct tggaagagga acaaaaagtg attgatagtt
    68041 tgatgtattc attccagcac tctttgagaa tgcaaaaaca tatgacgttt ttgatcgaaa
    68101 aagggggaat gtatctcacg aacaatcagc atgtgctgtt tcatggctgt atacctgtag
    68161 acgagaaggg acagctctta gcatttgaat tagaccaatc ttatcgcgga aaagaactgc
    68221 tgggcttttt tgaaaaacag ataactgaaa gcgcaaaaga tctgactgca aaagaagatt
    68281 tagcaaccga tttgatttgg tatgcgtgga gtgggccatt ttcgccttta ttcggcaaga
    68341 gtaaaatggc gacttttgaa cgttattttc tcacagagcc tcatacgcac gtcgagaaaa
    68401 gcaatcctta ttatcacttg cgagatgaag agtggttcag tgaaaaaatt ttagcagaat
    68461 tcgacacaaa agaagaaggg tcagcaatca ttaatgggca cacgccagta aaagtaaaaa
    68521 aaggggagtc accgatcaaa gcaaatggaa aagtatttgt cattgacgga ggtttatcca
    68581 aagcttatca aaaaacgaca ggaattgccg gttattcact attatgcaat tcttatggtt
    68641 tccaaatcgt gacacattat ccgtttttgc ctattgaaca acaatttgca aaaaaatccg
    68701 accaaacaaa tgtgaaaaaa gtaatcgaac agcaactacc acgcaaactc aatcgtgata
    68761 caacaaaagg tatggaactg cagcagcaga ttacccagct caaaaggctg ttggactatc
    68821 gaaaggacga ttgagaagaa ttccagtgaa cattgttcca tagactggag actgagacaa
    68881 aagtcgtgtc gcagtctctt tttccggata aacggtgttc ttctgccaac tcctcggtca
    68941 caagtcacac tattcaacaa acatgaaaag ctgttttttg aaattgctcc ttgtgactca
    69001 gagttgaacg gcaggctcac gacctcttgg tataaacggt gttcaaaagc cagtttctcg
    69061 gtattaagcc aaatttatgc aaaaatgtga aaagccattt tcacaaaatt tttcttaata
    69121 ctcgaaacta aacagctttt tcacaacccc ctcagaaagt ctattaaaat tcattcacaa
    69181 agaaatcgaa taagcgctgt tcagatggat gtacacggtg ggtaagttcc ggatgccatt
    69241 gaacaccgag gattttatgc atatcatctt tgctttgaac cgcttcaatg agtccatcgt
    69301 tagaaaaagc gatggctttt aataaaggtg aaagatcttt gactgcctga tgatggtaag
    69361 aattcacaaa atatggatcg gggagaaggt tgcttaacaa actgtctgaa acaatcttga
    69421 cttcgtgagt cgtaaattgc ggggcagtgg gctgttgttc atgttttact gaccactttg
    69481 gatataatga aagatcttga taaagtgtac cttccaaaac gacattcaat agttggagtc
    69541 cccggcagac tgcaaaaatt ggttttcttt gtttcaatgc ttccaagatc agtgcctgtt
    69601 caaattggtc tcgttgaata tttgtttccg ttagctttgg atgaggatct tccatgtaaa
    69661 gttgtggact gacatcttga ccacctgcaa gaataagttt gtcgatttgt ccaatcagtt
    69721 gaggagctga atctggagac atgactggca gaataagagg caatccttga gcatctaaaa
    69781 ctgcatccac gaatccttgc ggtgtatagc tcacttgatt tccttggaaa gtatctgttg
    69841 ctcgaatcag ttgatttccg gcaatgccga tgattggttt catttgtatt ccccctaaag
    69901 tagacttatt gtaacaagga tttaaaaaaa attaaactat gtaagacttg cccctatact
    69961 tatctgttct tgctgtgtta ctatctgtaa agagaaattt cgtgtgaaat tcatgacgat
    70021 agttgaaaat attgcttaat ttgttataat aaaaagtaag cagaaataaa aaggttgggc
    70081 gtaagcttca aggatctagt tcttcaaggg gtgagaagga cattttattg aaggggcctg
    70141 cctatactaa gttatcttta gaggtacata ctttcaatga aaaaagaaag catgtacctc
    70201 ttttttaaat cgaaaggaag tgaagcaagt gttagaagct ttggaacaaa tccgaaaagc
    70261 agaagaagaa aatgaaaagc gtgctcaaac actgcggcag cagctgaaga tctatgcaac
    70321 agaaaaagag caggaattgg aaacgatcca aacaaactat cgacaacaac taaaacagtt
    70381 ggtggaagaa aaagaacaat ttgaaataca agaattagaa aaagaaaaaa tgaaattgaa
    70441 agcagaagca gacaaggtgc aagcaacttt gaaagatcaa tatcaaaagc atcagagtca
    70501 agcgatcggt gcgatcattg aaagggtgaa agaaacttat ggccgtcact aagatggaaa
    70561 aagtgaccct catttcagat aaaagaaatc aggagaggat tttacaagca gtacaaggaa
    70621 tccaaaacgt tgaaatccgg gatctgtatc aaaatagaac aaacaaccag tgggtggaga
    70681 agtattttcc agataccacg attattgata aagaagcaaa attaagcgct ttaggtaatc
    70741 ggttgaaaga catccaagaa gctatccaat tcatacaaca ccacggcgat agccgtcaaa
    70801 agaaactcca tttgaaacgt caagaacttt ccttacaaga tttggaagcc aattattccg
    70861 aagaaaattt cataaataaa ttgaaagaag tccttgattt aaaagaaaag tgggaacaac
    70921 taactgaaga acgtgatcgt ttagaagaag aggaagaatg gcttcttcat tggcaacagc
    70981 tggatattgc tccaagtatg tatgcaagta agacgaccat ttttgagatg gggactgtga
    71041 gtgcagcaaa ttttgaaagt ttcaaggaag aactgagtaa gcttgatgac gtttatatgg
    71101 aagaaataga ttatacgcca aaacaagttt atctttcgta tgttgcttta aaaaaatcag
    71161 ttgctgttat agaagaaatt gctgggaaat acgggttcaa taaagaaacg tatccttatg
    71221 aacaaaatcc taaagaaaaa ctgcaagaga ccaagcagca attatccatt gttcacgagc
    71281 agcaaaagaa gataacgaca gcgttaggtc agtgcagtgg ctatatccaa gaatttgaat
    71341 gggtagaaga agtcacactt gccgttgcag aacgagaaaa aataaaagaa cggtttatcc
    71401 atgcgactta tctgatcgtt ttgcaaggtt gggtagatgc agaagaaaaa cagaatttgc
    71461 tgcatgtgtt aaacgaaacc gtatcagaag aagatatata cgtcagtttc gaaacaccaa
    71521 actcagaaga gattcagcag gaagtcccta ctaaattgaa aaaccatccg cttgtcgaac
    71581 cttttgaatt gctgactgaa atgtacagtc tgccaaaata tgaagaagta gatccaacac
    71641 cttggatgac accattttat cttgtcttct tcgggatgat ggtcgcagat gttggttatg
    71701 gtttgctgat gctcattggg acaatactag ctcaaaaact acttgttcta ccacgcggga
    71761 tgaaacgttt cgtaaaattc tttgagattc tctcgatacc atcgatcatc tggggattga
    71821 tctacagttc atttttcgga cagtcattac caaaagaact gtttggcatc cgcttgcctt
    71881 tcccaatcct atctacgacg gatgacgtca atacgatttt gattttatcc gtgattttcg
    71941 gtctgataca gattttggtt ggattatttg tggcagcaaa ggaaaatatc aggagaaaag
    72001 attacttgag tgctgtcagt gatggatttg cctggcaagg gatcctaatt gggatcgtta
    72061 ttgcgttggt tggtgccatg ttgataaaaa atacagcatt tgtttacctt ggcgctgggc
    72121 tatcgatcat tgctgcctta tgtatcttga tcgttccgat tatccaaacg ccgtctaaag
    72181 tcaaaggtgc tgccaaaggt gcgtataatc tttacggact gaccggctat atcggtgatt
    72241 tagtcagtta tacacgtttg atggcattgg gtatttcagg cggaagtatt ggagcggcat
    72301 tcaatatgct agttgctttc atgccgccag ctgctcgatt cagtgtaggg atccttttga
    72361 tcatcgcttt acacgcactg aatatgtttt tgacactgct aagtgcctat gtccatggcg
    72421 cacgattgca gtatgtcgaa ttttttggga agttttatac aggaggcgga cgtgcctttg
    72481 atccgctgaa aacggcggaa aaatacgtca acatcaatca caagaaaaaa aacaataaat
    72541 aattggagga tttttatata atgatggatt acttaattag tcaaaatggt ggaatggtat
    72601 ttgcggtatt agcgatggca acagcgacga ttttttcagg gatcggttca gctaaaggtg
    72661 ttggaatgac aggtgaggca gcagcagcat tgacaacaag tcaacccgaa aaattcgggc
    72721 aggctctgat tctgcaattg cttccaggta cgcaaggttt gtatggattc gttattgcct
    72781 ttttgatttt tatcaactta ggaaatgaca tgtcagttgt acaaggactg aactttctag
    72841 gtgcttctct gccgattgct tttacaggct tgttttcagg tattgcccaa ggaaaagtcg
    72901 cagcggcagg tattcaaatt ttagcgaaaa aacctgaaca tgctacaaaa ggaatcattt
    72961 ttgctgcgat ggttgaaaca tacgcgattt taggtttcgt tatttccttc ctattagtgt
    73021 tgaatgccta agcataaagg aggaaaagca gatgaacgcc attgaaaaaa tcatctctca
    73081 gatgaacgaa gcagcagagc aggaaagagc ggcgttagaa caagaagagc gaatgaagat
    73141 cgatcaaaac tttgaacaaa aacgtacgca agttgaaaca gaacatcaaa aacaaaaaga
    73201 aaaacaaatc gaattactcg aaaaaaaata ccgtcagcta aggaatcgcc agcaagtaga
    73261 agtacgacaa gaaaatttga atgcgaagca ggaattttta agacgattgt ttgcagatgc
    73321 ggtaactgaa atggaaaatt gggatgaatc tgaacaaatc cagtttatca aaaatgcatt
    73381 gtattcatta cctttgacag gaaaagtagc attcattgct ggagaaaaat cagcagccta
    73441 tctttctcag acgttgttgg atgaatggaa caatgaactt ccctttatga tggttttaag
    73501 tgatgaaact gttgctgatc aagcgggttt tctgataaac gatcaaggag ttcagtataa
    73561 ttttcttttc agcagccttg tacaagatat ccaaggaacg atgagttttg agattgccaa
    73621 tcaattattc gaataaggag ggatcagtgt atggaatacc acgaactaaa tcccttgatt
    73681 cgcggaagag agcttgaact tttgtccaat gagacatttg aacggatgat tcaaacaaat
    73741 tctgtcgaag cacttggtga aatattgaaa tccacgattt acagtccata tatttatgac
    73801 ggatatgaat atgacttcga acagaatctt ttaaaggagc agagccgtct atttcgctgg
    73861 ttaaaggaat cagcaccgga gcctgaaatc gtttggatct atacgatgcg ttatactttc
    73921 cataatctga aggttttgac aaaagctgag atgacaaatc aaaatctgga ccatttgtat
    73981 atcgacgatg gattttattc attagaaacc ttgaaggatg ccattcatac gcaagtttca
    74041 aatgaacttc ctgaaagcat tatggacagt atacgagaag tacgtgatta ttttgaagag
    74101 tctaccattc ttcagggaat tgacgtgatt tatgatcgac agtttttgac agagcagcga
    74161 cggcttgccg ataagctagg atatcaagaa ttgctgacag aagtcattgc ttttattgac
    74221 ttgaccaata tcacaacgat ggctcgagga atattgcaga aaagaacata tgggttcatg
    74281 acgacggtac tttccagctc gggaagtatc gaaaaagaag tatttttgaa ctttgtagat
    74341 aaagatatgg aagattacac gaattttcta ttagagacag attatgctga ttttctgcgc
    74401 cctgtcatcc gtgagggaaa tattgatctc gtaaagcttg aacagttgaa agatgattat
    74461 ctaagttcat tgtatcaaga agcacaaacc caggctttcg gaccactgcc actactagcc
    74521 tttttgaacg caaaagaggt agaaagcaaa aaccttcgtt tactggttat cggcaaaaga
    74581 agccatttcg aatcagaaac aatcagaaag cgggtgagac aagtctatga cgcataagat
    74641 cggtgtagtt ggcgacaaag attctgtttt gccattcaaa ttgtttggct ttgatgttcg
    74701 ttatggtaca accaagcaaa ccgttcgtca ggcaattgaa gaaatggcaa agaatgagta
    74761 tggcgtgatt tatgtcacag agcaatgtgc agcattgagc ccagaaacga tcgaacgtta
    74821 caaggaacaa atgatacctg ctatcgtatt gattcccaat catcaaggat cattagggat
    74881 agggctggaa gaaattcaaa aaaacgtaga aaaagcagta ggtcaaaata tcttatagta
    74941 tgaaaaggag atagaaattt tgcaaattgg aaaaatcata aaagtctccg gacctcttgt
    75001 aatggctgag aacatgtctg atgccagtat ccaagacatg tgtctagtgg gagatctagg
    75061 agttatcgga gaaattatcg agatgcgtgg cgatgtggcg tctattcaag tatatgaaga
    75121 gacttctgga attggtccag gcgaaccggt tcgctctact ggtgaagctt tatctgttga
    75181 attaggacca gggatcattt cacaaatgtt tgacggaatc caacggccat tagatacctt
    75241 tatggaaatc acacaaagta atttcttagg gcgcggagtt caattgcccg cattggacca
    75301 tgagaaaaaa tggtggttcg aaccaacagt agaagccgga gaaacggtga gtgctggaga
    75361 tgttatcggg atcgtagaag aaacaaaagt tataaaacat aaaatcatgg tgccaaatgg
    75421 gataaaagga acgatcaaaa caatcgaaaa aggttcattc actattgatg aaacagtatg
    75481 tgtcattgaa accgaaaatg gtgacaaaaa tttgacgatg cttcaaaaat ggccagtaag
    75541 acgtagtcgg ccaatcaaag aaaaattaaa cccggatgca cccatgatca ctggacagcg
    75601 agtgattgat acattcttcc cagtgacaaa aggaggagca gcagctgtac ctggtccttt
    75661 tggtgcaggg aaaacagtcg ttcagcatca aattgcaaaa tgggccgatg tggacatggt
    75721 tgtctatgtg ggatgtggcg agcgtggaaa tgagatgacg gatgttttaa atgaattccc
    75781 tgaattgatt gaccctaata caggagaatc gttgatggaa cgtacggtat tgattgctaa
    75841 tacatcaaac atgccagtag cagctcgaga agcttctatc tatacgggaa tcacaattgc
    75901 ggaatatttc cgtgacatgg gttatgacgt tgctatcatg gcagactcca cttcacgatg
    75961 ggcagaagca cttcgtgaga tgagcggacg tttggaagaa atgcctggtg atgaaggtta
    76021 tccagcttat cttggttcac ggcttgcgga atactatgaa cgatctggaa gagtcattgc
    76081 attaggatca gaaggtcgcg aaggaagtat aaccgcaatc agtgctgtat ccccgtctgg
    76141 aggggatatt tcggaaccag tcacacaaaa tacattgcgg gtcgtaaaag tattctgggg
    76201 tcttgattcc agtttagccc aaaaaagaca ttttccttcg atcgattgga tacaaagcta
    76261 ttctctttat tcaacagaag tagggaagta tatggatcat atccttcaag ctgactgggc
    76321 atccatggtc accgagggca tgcgtatttt gcaggaagaa cagcagctga atgaaatcgt
    76381 gcgtttggta gggattgatt ccctttcaga taatgaccgt ctgacattag aagtggcaaa
    76441 atccattcgt gaagattatt tgcagcaaaa tgcttttgat gatgtcgata cattcacttc
    76501 aagagagaaa caattcaaaa tgttgaagat gatcttgacc tttggtgaag aaggacgccg
    76561 tgcgttgtcg ttaggtgctt atttaagtga gatcatggac ggtaccgtat cgattcgtga
    76621 aaggatcgga cgaagcaaat atatcccgga aacagaatta gaaaaactag atcaaattat
    76681 tggtgaaatc aagcaaacga tgcaaacgat cgtttcagaa ggaggcatga gcaatgatta
    76741 aagaatatcg tacgatcggt gaagtcgtcg gtccattgat ggcggttgaa aaagttgctg
    76801 gtgtcaaata tgaagaatta atcgaagtcc gtatgcaaaa tggcgagatt cgtcgtggac
    76861 aagtattgga agtccaagaa gataaagcaa tggtccaaat attcgaagga acaagtggta
    76921 tcaatttacg tgactcttct gtacgatttt taggccatcc tttagaacta ggtgtatcgg
    76981 aagacatgat cggtcgagta ttcgatggtt tgggacgacc gaaagataac ggaccagaga
    77041 tcctgcctga aaagcatttg gatattaacg gagaagtaat caatccagta gctcgggatt
    77101 atccagatga gtttatccaa acaggtatct ctgcaatcga tcatttgaat acattagttc
    77161 gtgggcaaaa attaccagta ttttctggct ctggtcttcc tcataaagaa ttagctgctc
    77221 agatcgcgcg acaagcaacg gtactcgatt catcagatga tttcgccgta gtctttgctg
    77281 ctattggaat cacttttgaa gaagctgaat ttttcatgga agattttcgt caaacgggcg
    77341 caatcgaccg ttctgtcatg tttatgaatt tagcaaacga ccctgcaatc gaacgtatcg
    77401 ctactcctcg tatggctttg actgctgcag aatatcttgc ttatgaaaag ggaatgcatg
    77461 ttttggttat catgacagac atgaccaact atgcagaggc attgcgagag atttctgcag
    77521 cacggcgaga agttccagga agaagaggat atcctgggta tctttacacg aacttagcta
    77581 ctttattcga acgagcagga agaatccgcg gactaaaagg atcggttact caaattccga
    77641 ttctaacaat gccagaagac gataaaactc atccaatccc tgacttgact ggttatatta
    77701 cagaaggaca gatcatcttg actagagagc tttacaagag tgggattcag ccaccaattg
    77761 atgtactccc gtctctttcg cggttaaaag ataagggaac tggacccgga aagacaagag
    77821 aagaccatgc agctacgatg aatcagcttt ttgcagccta tgcacaagga aaacaggcaa
    77881 aagaattagc cgtagtttta ggtgaatctg ctttatctga tgtagacaag atctatgcga
    77941 agtttgctga gcggtttgaa aaagaatatg tcaatcaagg attctacacg aaccgttcga
    78001 ttactgaaac acttgatcta gggtgggaac tgctatcgat gttgccaaga accgaattaa
    78061 aacgaatcaa agacgacatg ctggacaaat atctgccaga agggaagtga gttctgatgg
    78121 cacgattgaa tgtgaatccg acccgcatgg aattgactcg attgaaaaaa cagttgacaa
    78181 ctgccacaag agggcataaa ttattaaaag ataagcaaga tgaactgatg cgtcaattca
    78241 tcctgttgat tcgaaagaac aaccagctgc gtcaagaaat ggaagagtct atgacagaag
    78301 caatgtctga ttttgtattg gcaaatgctt cgatcacgga acctttcatc gaagaattat
    78361 ttgttttgcc ggcggaaaac gttgatttgt ctatcgtaga caaaaatatc atgagtgtaa
    78421 aagtacctgt gatgaatttt gaatatgatg aacagctgac agacacacct ttggaatacg
    78481 ggtatttgaa ttccagtgcc gaactggatc ggtcaatcga tcggtttact accctgcttc
    78541 caaaactttt ggaactgaca gaaatcgaaa aaacttgtca actgatggcg agtgaaatcg
    78601 aaaagacacg tcggcgagtc aatgcattag aatatatgac gatccctcaa ttagaagaaa
    78661 cgatctatta tattcgtatg aagctggaag aaaacgagcg cgcagaagta acaaggttga
    78721 tcaaagtaaa aaatatgggt accactgact aagtggtatc tttttttaaa agaggaaaac
    78781 gatgattaaa caaagcaaac aaataaagaa acgtttgccg gctgttcagc ttctcgcatt
    78841 aggatttttt ctcctgattt tagttggcgg cggcctactt accctaccat tttttagtaa
    78901 tagtgggcat gggacaaatt ttatcgatgc gcttttcact gcaacttctg ctgtctgtgt
    78961 aacgggattg acaacattga atacagcaga gcattggaac agtgctggtc aatttttgat
    79021 catgcttttg attgagatcg gcggtcttgg ttttatgatg gttccgattc tatgctttgc
    79081 attgataaag aagaaagtca gtctcagcac acgtattata ttaaaagaag cgctgaatct
    79141 agaagaagta tcaggtgtga tgaagctcat gctttacatc ctaaaattgg cctttgtgat
    79201 ccaattgatt ggtacggttg ctttatcttt tgtatttgtg cctgaatttg gctggttgaa
    79261 aggtatctgg tacagtttgt tccatgcaat ctctagtttt tgtaatgcag ggtttgattt
    79321 gttaggagac agtttagcca atcaccagca aaatgtttat ttgatcatgg tggtatctgc
    79381 tttgatcatt tctggtgggt taggcttcat cgtatggcgt gatttactgg aatatcatca
    79441 gaaaaagaag atgacgattc attcaaaaat cgcattaacc gttacaggta cccttttatt
    79501 aggcggattc gttgtgttct ttttcacaga gcgcaatggg gcaacgctag tggaagggaa
    79561 tatgtttcaa cgattggcaa acaccttttt tatgagtgtg acacctagaa cggctggata
    79621 ttattcagtt gattacctcc atatgagcca tgcaggtctg attttaacga tgtttttgat
    79681 gtacatcggc ggaacatctg ggtcgacggc cggcggattg aaaacaacta cgttaggtgt
    79741 gttgctgatc cagatgcgtt cgatgttgaa aggacgtacg agagcggaag catttggccg
    79801 taccatccga caaggcgcag tgttgcgggc acttacttta ttttttgtta cgttgacgtt
    79861 gtgtgtagtc gccatcatga ttttatcggt gactgaaacg atacctaaaa cttctggcat
    79921 cgaatatatt gcttttgagg tcttctctgc gtttggtaca gtaggtctta cgatgggatt
    79981 gacgccagac ttgactgcaa tagggaaatt gatcattata gcgcttatgt acattggacg
    80041 agttggtgta ttgactgtgg tcttctctct gatgacaaaa gcacaaagaa cacaatcaaa
    80101 atacaaatat ccagaagaaa gtgtcatgat tggataattt catcttctga cagaaaccta
    80161 gtgatttagt aaaataagaa aataaaataa tttttcttgc ttcttggata ctttaagtgc
    80221 aaaatgttcg tttatttttt gaggcactac ccgttgttga acctcgggta gtgcctcttt
    80281 tttattgaaa ggaatcaata agatttgatt cctttattta tcaataggtt ataattcata
    80341 tttttttatt ttttctttta cgatacttag tccagaagct aaaatttcaa ggttattaga
    80401 ggatagggaa attcgaacga atgcatctgg ttgcgtacgt gtagtaaagc gtctggaatg
    80461 ataaagtcgg ataccgttag caagaaagtc attctcaatt atcttagggt caatctcagg
    80521 atcaactgga agaattcgat agtaagggac acgattcgtt ggtttttcta ctttgaaaag
    80581 agaatcaaaa agctgattgg ctctttttgt aagacttaat ttagtttcta agatcctatt
    80641 tgcttcattt gaatggagga tttgagtgat gacttccaca tctaatccag aagttttcac
    80701 atttatatta aaaattgctt gttctatctt gctttggaca tgtactggaa ctacaagata
    80761 agcaactcgc aatccagaac aaataaattt tgtcattcca gctaagtaga ttgtttggtt
    80821 aggtaataaa ttttggaaag gaggaatgat cgtatgttga tagtaagttg ttaaaaatga
    80881 atggatatca tcttcaataa cccatagacc ttctttttca atcacttcgg ctaattgctc
    80941 tcgtcttttg tgagagattt gaaaccctat aggattatta caagaaggca taagaaagac
    81001 accatgaatc ttcttttttc tacattcctg aatcaatata tcaggtcgca ttccttgctc
    81061 gtcaaaatca attggaacga tctctaaatg atataactga gccaattcaa tgaaattagc
    81121 gtatgtatat cggtctgttg ctattctatc acctggagag aaaagtgcag aaagtgtgat
    81181 agccaatcca ttttgaacac cggaaacaat agatagagat tctaaggacg gaatgactcc
    81241 ttgagttttt aaccaatttg ctgcagtagt caattgaaaa tttgtgcctt tgggatcttg
    81301 gtaattaagc aaaccaacaa gatttgggtt attactagtt gattgaataa acgggacaac
    81361 gaatttatta cattcttcaa aagaactaac cagaccaaaa tcaatgatat gttcggttac
    81421 ttcattggta gagatggtga tggactcaaa gacattggga gaaataaatg tgccacttcc
    81481 aatattcgta tacagcaaac ctttctcaat tccatattta taagccttgc caacggtagt
    81541 aaaatttaaa tctaaataat cagctagctc tctttgggag ggtaactgag tgtttttctg
    81601 taatttattc tttaaaatat cttgttctaa ttgttgaatc aacgatcgat aataaggacg
    81661 cttcaatttt tgtttgtctg gtcgccaagt taacgggtaa ttgtcataag aattaatagg
    81721 cattttctcc actccttaaa attgtatacc atacaatttt accattgatt gtattgattt
    81781 tagaagttaa tcttttgtga ggagggaatt gtctatgaaa attattaatt ttgaggaagt
    81841 agtagcttct tttagttttg aagaagcgat tcaagtaatg aaaaaatgtt tttttgatta
    81901 tgaaagtggt aaaatcagcc aaaatcctcg attggtggaa attttacctg acggaaaagg
    81961 caaaaatatg tttgcgatga tgcctgctta tttgggggag aacagatatt ttggcacaaa
    82021 agtcattacc gcttttccgg ataatcatca gtatcattta ccttctcatt taggagagat
    82081 tttcttgttt gatgctcaaa acggactacc caaagcattg ataaacgcaa atgcagtcac
    82141 ctggattcga acagctgctg tatctgcgct ggcaacagat tacttagcaa atccgcatgc
    82201 agaaacactt gctttaattg gagctggtca acaagcattg tcgcatttga ctgcgatttc
    82261 taccattcgt cccatcacta cagtgtttgt ttacgatatt ttagaagaaa gacgaaatac
    82321 ttttatcgct aaagctaaaa aacagtatcc taaaataacg tttataaatg caatatcagt
    82381 agaagatgca gtacgtccag cggaaatcat ttgtacgttg acctctagta aagaggcatt
    82441 tcttcaaaaa gagtgggtaa gggaagacgc acatatcaat gctgtgggca catttactcc
    82501 tgatacaaga gagataagta catcgttgat ggaaacttct caaatctatg tagatgatta
    82561 ctcagctgcc ttaacagaaa gcggggacct gcttatagcc atggcagaag gagatttaga
    82621 ggaatcttgt attgttggta cattaggcga attagtttct gagaaaaaaa cattttataa
    82681 aggagaatcg aggccgacga ttttcgacgc agttggtcta gctattgaag atctttgttg
    82741 tgcagaatat atttataaca aattagagaa aggggcttta aaatgaactt agattattac
    82801 gtagttgatg catttgcaga tgaggtgttc aaaggaaatc cagcagcggt ttatgtgcta
    82861 gaagaatggc tgccagaagg tacgatgcaa aaaattgcca ttgaaaacaa tttatctgaa
    82921 acagcattta cagtgaagaa aaaccaagaa tttgagctta gatggtttac gccagatcga
    82981 gaaatcgatc tttgtggtca cgcgacatta gcaacagcat ttgttttatt taactattac
    83041 aaaatacctg atgaaacgat taaattttct actcaaagcg gtaatctatt tgtgacaaga
    83101 attaaagatt attattacat ggattttcct agtattatgc cgaaaaaagt gccgatattg
    83161 tctgaatatg aagaagcgat tggggctaag attaaagaag cctacttagc acgtgattta
    83221 tttttcgtat tagaagatga aaaaacggtg gctaaattgc agccagattt tacagctatc
    83281 aaaaattttg aattaggaat aggagtcatc gtgacagcag aaagtgtgca aaaagacttt
    83341 gtttctagaa ccttctttcc aaaattaaca atcaatgaag atccagtatg tggatcagca
    83401 cattctaatc ttattcctta ttgggcagaa agaatgaata aaacaaaatt gtcagcttat
    83461 caaatgtcat caaggggcgg ggatctagaa tgtgaattaa aagaagatcg tgtgataata
    83521 ggaggaaaag cagttttgtt ttctaaaggt acctcgttta tttgatttct ttcaaatgaa
    83581 ttaaaaataa attggtggag agtggcaacc ttatagctac aatggttgtt aaaaaaagac
    83641 gcaaactttt tcaatttaat gaaaagtttg cgtctcattt taattattgg tgtttgtttt
    83701 ttattcaatc aagagtaaaa tgccccatac taactatcct cggatgactt cgattttata
    83761 accatcaggg tcaatgacaa agtagtaaga aggcgcagtg ccaggtaatc cttttaagtc
    83821 agtaatctca agaccagcag ctttatgttg ctcgtgtaat ttttctagat catcagctgc
    83881 tatagcgata tgaccatagc cgtctcctag atcatacgca ccatgatcat agttgtaagt
    83941 caactctaat tcatagtcat ccccaggaag tgtcaaataa acaagtgtaa atttgttttc
    84001 tggaaaatca cggcggcggc tttcttcaaa accaaatgcc ttttgataaa attcgattga
    84061 tgcttcaaga tctttcactc gaacacatgt gtgagccatt ttcatagatt catctctcct
    84121 tttcctttta ctcaatcata acaaaaagaa gtaaggaaat agttgatttg tgcttgttca
    84181 tgagaaattt ctcatgtaaa atggatagaa aataaaactg ttaaggtgga aactattcat
    84241 ggataaacca atttttggtg aaaaaaagca atttgctgat tatcaaaccc gttatgcagc
    84301 atatattgtc atagaaagag atcagcagat tgcagtaatc gaggctccaa atggtgcttt
    84361 ttttcttcct ggcggggaaa ttgaaggaga agagacgaaa gaagaagcga ttgtccgtga
    84421 gctgatagaa gaaatgggaa ttgtggcaga aatttcttct tatctgggag aagctgatga
    84481 gtatttttac tcgaattatc gtgcaactta ttattataat ccaggatatt tctttgttgc
    84541 tgattcttgg aagcgtatag gagaaccaac tgaaaagacc aataaaattt ggtgggtgag
    84601 ccctcaagaa gcgcttgtga aattgaaacg tggtagccac cgatgggcag tcgagagatg
    84661 gatcgaaaaa aataaaaact gaaagaaagc gagtagatca gctttctctc agttttttta
    84721 tttattacag tacatatttt ttgattgctt cagctacacc gtgctcgtcg tttgatgaag
    84781 tgatgacatt tgctgcttct ttgacaagag aaacggcatt tcccattgct actcctagac
    84841 cagcatattc aatcatggat aagtcgttct cgttatctcc gatagccatg atttcatctt
    84901 gatcaatacc aaggtgctgt gcgagattcg caactgccgc acctttactt gcttctttat
    84961 ttagtacttc gaagtaatag ggcgcacttt tgaccgttgt gtatttatca cggaaagttt
    85021 gcggtaaacg cgcaattgct gcatctaaaa tttctggttc gtcgatcatc atcattttga
    85081 cgatgttcat gtctggtgtc atttcttcag gtgttcgata ttttagcggc atcttgacta
    85141 aacttgcttc gtggatcgta taagcgctga tatctcgatt aggcgtgtag atcgctttgt
    85201 catcgatcga atggagatga ctgccgacac ggcgtgccat cacttcgatc tctaggaaat
    85261 cttcatgtgt cagtccgtga cgggcgatga ttttacctga tttcgtttgt tgtacgagtg
    85321 ccccgttata agtaataacg taatcattat cttgaaaaag gttcaactcg tttaactgtt
    85381 cctggacacc tggtaaggga cggccagtgc ataaaacgat cgaaacacct tttgcaacag
    85441 cagcggcaat cgcttctttt acttcttttg ttacttctct tttctcattt aataaagtac
    85501 catcgatgtc gatagcgact agtttgatag acataatttc ctccaatata ttttaattta
    85561 tttcaatcaa tgcgccatta cggatatggc tggcaaattc ttgataagtt tcgtcaaata
    85621 aatcataatg atcacgcaag gacggatcaa ccatttcttt tggaaagaaa aatcgctcat
    85681 ctccttgttc ttgtcccgct aatgcagcaa caagctgact aacctgtgat aattctatca
    85741 aagttccgtc atttcgcaaa atctcgattt gtgtgcgatg tctgccttgt tctgggcgat
    85801 aaaaatcata aggtagatcg tagctagaat tcacagcagt gtaataaaca ggattatatc
    85861 cgaccttttc tactaacaaa gtgagttctt ggactaattt tgaatcccgg ttgccagaaa
    85921 aagtagcaga tttcagtggt ttacgattca aaaaacgttt agctaaatca tttaaaatag
    85981 gatcagcaga ttcagaccat tgagtaaagt aagtgcccaa gaccccatca tcaagtttga
    86041 ggtattcttc taaagtaaag cttccgtcaa gaaatggcag aagtaattga gaatggagtt
    86101 caaaactgct ggcatcttct tgatacaact cgtgtgcgcg atgaagcaga tgctccaaaa
    86161 ttacttccat accacgggaa acaggatgga agtacacttg cacatacatt tgatatcggc
    86221 taacgatata atcttccact gcatgcatgc cactcatgga aaaagcgatc ccgccttcat
    86281 aaggacgaat cacacgtaat attcgggtaa gatcaaatgt tccatattct gttccagtga
    86341 aataagcatc ccgaagcaaa tagtccattc gatctgcatc gatctgacta gaaatcattt
    86401 ggacaacttg tgggttgggg taagtttttt ggatcacact agctaccttt tcaggaaaac
    86461 cttcttctac tcggttaaga atctgataca cttctgtttc tggagaagtg atgatctcta
    86521 cagtgatggc ttcatgattc gtatggaaga tatgttcaaa tgtatgagag taaggaccat
    86581 gaccgacatc atgcaacagt gccgcacata aagtcaccag ccgttcctga tcatcccagc
    86641 ctccgttgcc tattttttct tttgaaaaat ttcgagaaaa gatatcgcag attcgtcttg
    86701 aaatctcata aacgcctaat gaatgagtaa aacggctatg ttctgctccg tgaaaagtga
    86761 aagaagaagt gcctaattgt ttaatgcgtc gtagacgttg gacttctttc gaattgatca
    86821 aatctaagat tacttgatgc tggacatgga tatagttatg gactggatca cgaaaaactt
    86881 tttccatagg cagcatttga tatttataag gggttgtcat aatgtagctc ctttatgtta
    86941 ttacgttgtt ccattctctg gatttggttg ttccattgct ccagctggat ttcttttttt
    87001 agccagtcgg tagaatcctg taaatcactt gcagaataca gcttttcaaa agcttttgca
    87061 aatgcttctt tgacttcttt tacaaaaagg ggttggtcaa gcagttcttc aagtgttgcc
    87121 attgtagcag gatccactgc aggatagcca tttgtaccaa agtcttcttt taaagcagat
    87181 tgataaaatc gcttcatgac ttgcccgcgt ttttcctgat taccgttgac acttaagtac
    87241 atcataacag aaatcccatt tttgatccga cgctgagcga taccggcaaa ttttttgcca
    87301 tggatgctta aatcataagt tccaggacaa taagaatggg tgatttcgaa tgcttgtatc
    87361 ggtgcctttg gaaaagcgta tttagtcaat aggagcattt gctcgtaagc gtcatctatg
    87421 gttatttttt ttgtgtcact ttgggggaaa atcaaagaaa tattcaaaat tccttcatct
    87481 gatacgatcc ctaatccacc tgaatttctg atcactggag cgtaacctgc atctttcagt
    87541 gtttggatcc cgtcacttag aaaaggagtt cgaacatcct tcatacctaa gatgactgtg
    87601 cagtcatatt gccagaaatg taaaaagatt tgctggtgtt ctttagaata ttccgttaat
    87661 atatctgtta atgcaaaagg ggaaaagtcg tttttttgat aaatgtgctg atctagtcga
    87721 atcgcagggt tcattcaacc ggctcctttg tataaattca tattttctac aatattatac
    87781 tctaaaatca aagagttgtt ttggaaaaaa ggttttctgt aaaaatggac aagttggttg
    87841 acaaaacaaa agaaactagg aacaatgtag ctatgagaag taaaggagaa tgaataatgg
    87901 aattatcaca aggaacaccg atttcaatca aattgcgtac aaaagtaaaa cagcaagggg
    87961 aagtgcaaga tttttatttt gatttgaaag ggcaaatggt gacaattggc gatacgctgt
    88021 acatacgtta taaagagatt caggaggaga cgggcgatga gattcctgta acgatcaaat
    88081 tagttccaga tggtcatgta cagctgatcc gtgcaggaga aatgcggatg cgtttgaaat
    88141 ttggctataa agaacggtta gaaacatcct atcgaacacc atacggtatg atccagattg
    88201 ctacttttac gaaagaactg catgttagtt tgaaagaccg tcccacagca gggaaggttc
    88261 gaatcgatta tgatctgttc atgggaccgg aaaaaatcgg cgaatattat ttgaccttgg
    88321 attttacagc gtaaatataa aagaaaagtt tgattttttg tgtctgattt tgtatgataa
    88381 ggagtagact gtgaaaggac gtgtcctgat tggaaattag tgtatttgaa ggtgcaaaca
    88441 aaagcgaatt atcaatgatc gaagtagcac acgcgatctt agaacaacgt ggcgatgtca
    88501 tggatttttc tgatttagct aaccagatcc aaaactatct tgggaaatca gacagtgaaa
    88561 ttcgtgattc actcgcacaa ttttatactg acttgaatat tgacggcagc tttatctcat
    88621 taggggataa tcgttggggc ttgcgttcat ggtatgctat cgattcgatc gatgaagaag
    88681 taaaccatgg aatggacgaa gaggatgaag atactccacg tcgccgtaaa cgcaagaaag
    88741 tcaacgcctt tatcaatgat gatgaagacg cgatcgatta caatgacgat gacccagaag
    88801 atacagatct gacagaagaa gatgacgatg atctgtttga tgacgatgat gatgaagatg
    88861 aagaaatcgc tgcttataat tctgacttgc aagaaatcgg agcagatgac ccgacagatg
    88921 atgaagaaga cttgccagga aatatcgaag aagatttaag tatcatagac gaagacgatg
    88981 atgatgaaga ttatgaagag gaagaatttt ctgactctga aaaagaataa acattatttt
    89041 cttacgagag gttgtgacag agacgatcag cctatcagaa taaggaggtg tgacacgatt
    89101 tttgtcacac cttctttttt gtatataaaa atcccaagta aatacataaa aaataaaaaa
    89161 attgaaactt ttagcgcacc ttgtacgtca tattaagtga ggcagaaagg agtggagaat
    89221 gatttttact ggatataaga aaaaacggca gatcgaaaaa taccgtcacc tgctgaatgg
    89281 actcatcgaa actgattatc aaaaaatgtt ccgtatcacg atgaattacg tacatgataa
    89341 agaagaggcc ttagatgtga tgcaagatag ttttcataaa gccctgaact attttgaaaa
    89401 agaaaaagag atcgagcatt ttagtgcatg gttttatcgg atattgatcc gcacggcatt
    89461 agatagttgg cgcagacaaa aaaggacgcc tgtggactta tttgaaattc aagatatccc
    89521 ccaacagtta cctttagaaa cttccgttgc agagctccat atgattttag ataaaataga
    89581 cagtccggaa aaagagattc tgatcctcta cttttttgaa ggatttcgtt tgaaagaaat
    89641 tgcgactatc ttggatatga atgaaaacac agttaaaacg aaaatgtacc gctcattgaa
    89701 tgctttacgt caaatcctag aatgaaggag agaagagtgc catgtattcc caaaaagaaa
    89761 atttaaaaaa actaaagaaa acttataaag aacaagcaat tcccaaaggg cttaaagagg
    89821 agatccgcca gcgttttatt atcgaagagc aatcatttat aaaagcaaag cggagaaaga
    89881 gacgtctcca agggttcgga gtatctttgg ctatcgtaat gattacaaca agtagccttc
    89941 tttttaatag tcaagttcga agctttgcag aagatttacc catcttagga tcagtcattg
    90001 aattgatctt aggcgaacgc tttactgatc gatctgaaaa aatcgatatt caggtaccga
    90061 agatcaatac acaaaatgag aaagaaaata aaacaattca tggattaaat caaaaatact
    90121 ttcgcgaagg acaagcagat tttgaaaaag ctgaaaaaga atatggaaat tttgaaacgg
    90181 atcattacca agttttggga gattatcaga aaatcgtaga cgataaccgg tttctcgtga
    90241 ttgaaaggca gatcacccaa acagcagcag atagccatgt tgaaaaaaga tacgacacaa
    90301 tcgacaagaa aaacagtgtc cagctctcac ttccactttt attcaaagat gacacctatc
    90361 tatctgtttt gacaaaagaa gtcaaacggc agatggccga acaagtcaag gaagatccat
    90421 ctaagtacta ttggactgag caagatattc aagaagggac gatagaaaaa ccaacacttg
    90481 ttacgcctac acgaagtttt tatttgaaca aagagcatca attagttttg actttttctc
    90541 aatacgaaat tgctcctggt tatatgggta caccagaatt tgtcattcct aagtccgtga
    90601 caaaaacgat tttggcatcg gaagattatt tggatcatta gacgagaagt agttgcgact
    90661 agcagtttca attttcaagt aagtacaagt aaataataag aagagaaaaa gagaaagaag
    90721 agatatagtg aaaagaaatt atttagttat cagtattatt gctttatccg tgcttttgtt
    90781 tttcgaaatt acttcggtgg cctccttgat caccgaagta aaaggacaaa cacactcttc
    90841 ggagcattcg tcacaatctt tcaaacaatc agaacaatca accattaaaa gccaatcaac
    90901 tgaaacagct aacgtcaaag aagccattca agaatcagcg gaaaaggtac aaagtcagtc
    90961 atcacaatca acacaagaca gtacaaaaac gagccgagat ccggctgtca aaagcgtcgc
    91021 aatcagcttt gatgatggac caggagcgac cacaacaccg cagcttctgc gaatcttaaa
    91081 agaaaaaaac gtgcatgcca cttttttcgt cttaggagaa aatacggcgc agcatcctga
    91141 aatcgtgaag cagacagctg aagcagggca cgagattggc aatcacacat acgatcatca
    91201 gaatctggcc actctttctg cccagtcgat gacggaggaa gtgacgaaag cagacactga
    91261 gatcaaaaaa gtcactggca aaacccccac ttttgttcgt ccgccctatg gttctgtcac
    91321 gaatgtagga gcaacgatca tccagcgacc gatcatcgaa tggtctgttg attcagaaga
    91381 ttggaagaca agaaatcctg atttgatttt gcagaagatc caagcaacag tatacgatgg
    91441 ggcaattatt ttattccatg atatctatcc tgaaacgatt cgtgccgttc ctcaagtgat
    91501 cgattatctg aaagagcaag ggtatcgcat cacgacagtt ggggatttat tagggcatcc
    91561 aacggctgtc gagaactatt atggcagaaa cgatcatcgg ccagttcagt aagtgagaca
    91621 agtatcttta ataaataaca cattttaata agtagttgaa cacataacta ccttacttgc
    91681 aaaggcttaa caatatctat acaggtacaa caaaaaactg tccaccaatt ttctttggcg
    91741 gacagttttc acttaaaaat aaagaagcaa ctattcgtaa attatgctaa aatagcagta
    91801 gaacgcgtcc tgagggaatc gaacccccgt ctcaagaacc ggaatcttac gtgatatcca
    91861 ctacactaag gacgcaagca ctgggataat tgtaactaaa cagataaaaa attacaaggg
    91921 gattggacaa aaaaatgaaa tttgagcaaa atttttcaca aaaacagcag cagacacaga
    91981 aactagccat gacgcaacag ttgcaacagt ccatccaagt tttacaatat aacagtgagg
    92041 agctgttggc attcgttgag aatcaagcaa tggaaaatcc attggtggaa gttgttgagc
    92101 cggaatggca gccagattac ataaaagctt cttcatcttc ttatgaagga gaagagacaa
    92161 attacctaaa tcagattcct gatacaaaag ggtctttatt tgattcattg atcgaacaag
    92221 tccatttgaa ttaccgagat acttttttga ggaaaatcgt tctttatctt gtagaataca
    92281 tcgatttgaa cggatttttg acgattagtt tagaagaagc cggaaaagag attggagcta
    92341 cgcctatcca aatgctcgat gcgttgactc tgattcagca attagaacca gctggagtag
    92401 gtgcacgtag tttgcaagaa tgtctcatgc ttcaaacaga acgagatgat tatgcgccgg
    92461 aattagctta tatcgtgttg gaagaatgtt ttgaggaact ggtggagcga aaatggaaag
    92521 aaatcgcaca acgtttttct gttgatctac atgctgttca gcagatattc gactatttac
    92581 aaacgttgag tccaagccct ggccggattt tcgaccgtag cagcgaattg tacattattc
    92641 ctgatgtccg ggtattagtg gacgacgata aaaatgtaca agttatctca aaccgaaaaa
    92701 atcagccaaa tatacgcttt caagagagtt acttcaaaca aatgtcacaa caagcagata
    92761 aagaaactga aaattattta aaagaacgga aacaggaatt cgaatggttg aagaaaacga
    92821 tcctacaacg tggggataca attttgcggg ttgctcaagt cattgtttcc cggcaaaaag
    92881 ctttctttat cgacaaggaa cgtccaatca agcctcttac gttaaaggaa gttgcaactg
    92941 agatagatgt gcatgaatcc acggtcagtc gtgcagtcaa cgggaaatat ttggaaacag
    93001 acttcggtgt atttgaatta aaaaaatttt tcactacacg aattcctacg aatagtacag
    93061 aacagacaga agacctttca gcagatacag cgaagaaaaa gctgcaggaa ttagtcgatc
    93121 aagaagacaa aaacaagccg ttgtctgacc agaaattggt tgaattgctg aaaaaagatg
    93181 agatagcgat ctcaagacga acagtagcaa aatatcgtga tttattagga atcccgagtt
    93241 catcaaaaag aaaacgttat gataactaat cagtcaaaag actagaaaaa gtatttcact
    93301 ttttctagtc tttttagttg aaaaacgaga gcaaaactgt tagacttaca gtcgtgaggt
    93361 tgagatttcc ctttattgat gtgaaattcg aatagttaat ttcttttttt atcgtatatg
    93421 ggtcgtttta agtctacatt ggacgtaaag tgtccaacta aggagatcgc tatgctggat
    93481 gaaataaaaa tgattgaatc tgttgcacca gatattatcg aagtgttgca agaacgctat
    93541 aaaattttac gaaatatcta ttggatgcag ccgattggac gtcgaagttt atcggaaagt
    93601 atggggatca cagaacgagt attgcgaaca gaaacagatc ttttgaaaaa tctgaatttg
    93661 atcgacacct caaaaagcgg tatgacgctg acaactaagg gcgaagaagt ttatcaaagc
    93721 cttgaaaact tcatggatca gcttttaggt acgcatcaaa ccgaacaaca gttggcagaa
    93781 tattttggta accaacgctg tatcgttgtt tcaggaaaca gcgaagagca gaccaaagtg
    93841 gctgatgcat tcggcgaagc gctgagcgaa gccctagacc gactacttcc agaaggcgag
    93901 aatatcatcg ctgtgatggg aggaactact atggctgtgg tagccgaaca attgtctaat
    93961 ctggaaaata agaaacggca taacctgttt gtccctgcaa gaggcgggat tggtgaagcg
    94021 ataactgtac aggcaaattc tgtcagtgca agaatggctg ctaaagcaaa cggtaaccac
    94081 cgtgcacttt atgttccgga acaattaagt ctggcaactt acaattcttt actcaatgag
    94141 ccatctatcc aagaagtctt aaacttgatt agtgaagcga attgtgttat tcatagtatt
    94201 gggcgtgcat tacacatggc agcacgtcgt aagatgactg aaaaagaatt agtcatgctg
    94261 aaacaagcaa atgctgttgc ggaatctttt ggctactttt tcaatgaaaa aggtgaagtc
    94321 gtttataagg tccctagaat cggcatagaa ttgcaagatt tggaaaaagt cccgatcatc
    94381 atggcaatcg cgggcggaaa atcaaaagct aaagcaattc gagcttatat gaaaaatgca
    94441 ccaaaacaaa cgtggctcat cactgatgaa gctgctgcaa atgagatttt aaaaggggta
    94501 accctttaaa ataaatattt ttttgatttt cataaggagg aaatcttaaa tgacagttaa
    94561 agtaggtatt aatggttttg gacgtattgg acgtctagca ttccgtcgta tccaagatgt
    94621 agatggaatc gaagtagtag caatcaacga cttgacagat gctaaaatgt tggcacactt
    94681 gttaaaatac gacacaactc aaggacgttt caacggaaca gttgaagttc atgaaggttc
    94741 attcaacgta aacggaaaag aagttaaagt attagctaac cgcaacccag aagaattacc
    94801 atggggcgaa ctaggcgtag atatcgttct agaatgtact ggtttcttca cttctaaatc
    94861 agcagctgaa aaacatttaa cagctggtgc taaacgcgtt gttatctctg ctcctggcgg
    94921 aaacgatgtt ccaacaatcg tttataacac aaaccacgaa acattaactg gtaaagaaac
    94981 agttatctca ggtgcttcat gtactactaa ctgtttagct cctatggcta aaacattgaa
    95041 cgacaaattt ggtgttgttg aaggattaat gacaactatc cacgcttaca caggtgacca
    95101 aatgactcta gacggacctc atcctaaagg tgacttccgc cgtgcacgcg ctgctgctgc
    95161 aaacatcgtt cctaactcaa caggtgctgc taaagctatc ggtttagtaa ttcctgaatt
    95221 gaacggtaaa ttagacggag ctgctcaacg tgttcctgta ccaacaggtt cattaacaga
    95281 attagtaaca gttcttgaaa aagaagtaac tgttgacgaa atcaatgcag caatgaaaga
    95341 agcttcaaac gaatcttacg gatacaacac agacgaaatc gtttcttcag atatcgttgg
    95401 tatgacttac ggttcattat ttgatgctac acaaactaaa gtaatgacag ttggcgacaa
    95461 acaattagtt aaaactgttg cttggtacga caacgaaatg tcatacactg cacaattagt
    95521 acgtacttta gaatacttcg ctaacttata agatagacaa acgtacaagt gtaaattata
    95581 gacatgtaca tttaaaagcg gggaagcaat cgcgcttctt ccgctttttt tgataataag
    95641 tttattcagg aggaaataaa atggctaaga aaacagtgaa agatatcgat ttaaaagaca
    95701 aaaaagttct tgtccgtgtt gacttcaacg ttcctttgaa agacggcgtg atcactgacg
    95761 acactcgtat caaagcagca ttgccaacaa tcaactacgt tctagaacaa ggcggaaaag
    95821 caatcctttt ctctcacctt ggacgtgtaa aaacagaaga agataaagaa ggaaaatctt
    95881 tagctccagt tgcaaaacgt ctaggcgaat tattaggtaa agaagtaaca ttcgtacctg
    95941 aaactcgcgg agaacaatta gaagaagcaa tccgcaacat gaatgacggc gacgttgtcg
    96001 tattcgaaaa tacacgattt gaagatattg acggtaaaaa agaaagcaaa aatgatacag
    96061 aactaggtaa atactgggct tcattaggcg atgtatttgt taacgatgct ttcggtacag
    96121 ctcaccgtgc acatgcttct aacgtaggta tcgcttcaac tggtatccca acagttgctg
    96181 gattcttgat ggaaaaagaa atcaagttca tcggtgaagc agtagaagaa ccaaaacgac
    96241 caatgattgc aatccttggt ggcgcaaaag tttctgataa aatcggcgta atcgaaaact
    96301 tgattcctaa agcagataaa atcttgatcg gtggcggaat gacttacaca ttctacgaag
    96361 caaaaggaat caagatcggt aactctctag tagaagctga taaagtggaa ctagcaaaag
    96421 aattaatcga aaaagcaggc gacaaacttg tcttgccaat cgacaatgtc tgtgcaccag
    96481 aattttcaaa tgatgttgaa acacaagtaa tcgaaggaga cattcctgat ggtttgatgg
    96541 ctttagacat cggcccagca tcagtaaaat tatttgctga tacattacaa ggtgcaaaaa
    96601 cagttgtttg gaacggacct atgggtgtat ttgaaatgag taactttgct aaaggtacaa
    96661 tcggtgtttg tgaagcaatc gctaacttag aaggcgcaac aacaatcatc ggtggcggag
    96721 actcagccgc agcagcagaa caattaggat ttgctgacaa attcacacac atctcaacag
    96781 gtggcggcgc aagcttggaa ttgcttgaag gtaaagaatt accaggacta gcagcaatca
    96841 acgataaata aacgaccaag tttgctcaat tctgacgagt aatcgaacaa aaggtgaagg
    96901 gagttttcat gactccctag ccatttttca ataaattaag gagtgtgttc ctcaatgcgt
    96961 aaacctatta tcgcaggtaa ctggaaaatg aacaaaactg catcagaagc aaaagaattt
    97021 gctgaagcag taaaaaacaa aatcccttca aatgacaaag ttgattcagt catcggatct
    97081 cctgcattat tcttacaaga attaatgaca tcagctgaag gtacagaact aaaaatctca
    97141 gcacaaaact gctactggga aaactcaggt gcgttcactg gtgaaacttc accagcagct
    97201 cttgctgatc taggtgttca ttatgtgatc atcggacatt cagaacgtcg tgaatatttc
    97261 catgaaacag acgaagacat caataaaaaa gcgaaagcta tctttgcaaa tggtatgaca
    97321 ccaatcctat gctgtggcga atctttagaa acctatgaag caggcaaaac tgctgaatgg
    97381 atcgaaggac aaattacagc tggtttagct gatctttcag ctgaacaggt gtcaaacatg
    97441 gttattgctt atgaaccaat ctgggcaatc ggtactggta aatcagctga cgcacaaatc
    97501 gctgacgaaa tttgtggagt tgtacgtcaa acagttgaaa aactttatgg aaaagaagta
    97561 tcagaagcag ttcgtatcca atacggcggt tctgtaaaac cagaaaatat tgctgaatac
    97621 atggcaaaag aaaatgttga cggtgcttta gtcggtggcg caagcttgca agcagattca
    97681 ttcttgtcat tgttagacgc tgtaaaataa ttgttgaaaa tttgcgaaaa atgcaagaaa
    97741 tgtttgcact aatgagcaaa attaactaaa atcgtatatg agggaaatcc cttaggaaaa
    97801 aacaaactca aaggagagac aaaacatgtc aattattact gatgtttacg ctcgcgagat
    97861 cttagactca cgtggtaacc caacaatcga agtagaagta tacacagaaa gcggagcttt
    97921 tggccgcgga atggttcctt caggtgcttc aactggtgaa tacgaagcag ttgaattacg
    97981 tgacggagac aaagctcgtt atggcggtaa aggtgtaact aaagctgtcg acaacgtaaa
    98041 caacatcatt gctgaagcaa tcattggcta cgatgttcgt gaccaaatgg ctatcgacaa
    98101 agctatgatc gctttagacg gaactcctaa caaaggtaaa ttaggtgcga acgctatttt
    98161 aggtgtttct atcgctgttg cacgtgcagc tgctgactac ctagaagtac ctttatacca
    98221 ctacctaggc ggattcaaca caaaagtatt gccaacacca atgatgaaca tcatcaacgg
    98281 cggatcacat gctgataact caatcgactt ccaagaattc atgatcatgc ctgttggcgc
    98341 tccaacattc aaagaagctc tacgtatggg tgctgaagta ttccacgcat tagcttcaat
    98401 cttgaaagct cgcggattag caacttctgt tggtgacgaa ggtggatttg ctccaaacct
    98461 tggttcaaac gaagaaggtt ttgaagtaat catcgaagca atcgaaaaag ctggctatgt
    98521 acctggtaaa gacgttgttc ttgctatgga tgctgcttct tcagaattct acgacaaaga
    98581 aaaaggtgtt tacgtactag ctgattcagg cgaaggcgaa aaaacaactg acgaaatgat
    98641 caaattctac gaagaattag tttctaaata tccaatcatc tcaatcgaag acggattaga
    98701 cgaaaacgac tgggacggat tcaagaaatt aactgacgta ttaggcgaca aagttcaatt
    98761 agttggtgac gacttgttcg taacaaacac tcaaaaatta tctgaaggta tcgaaaaagg
    98821 aattgctaac tcaatcctta tcaaagtgaa ccaaatcggt acattaacag aaactttcga
    98881 agctatcgaa atggctaaag aagctggcta cacagcagtt gtatctcacc gttctggtga
    98941 aacagaagat tcaacaatct ctgatatcgc tgttgcaaca aacgctggcc aaatcaaaac
    99001 tggttctcta tcacgtactg accgtattgc taaatacaac caattactac gtatcgaaga
    99061 ccaacttggt gaagttgcag aatacaaagg tttgaaatct ttctacaact taaaaaacaa
    99121 ataattcttt tctaatgaag atacatgatc gactattgaa agatagtcga gtatgatcac
    99181 ttagttaagt aatcgaactc tctacttgct gaaatatcag caggtagaga gtttttttgc
    99241 aactagcttc tgtatatgat gtttacaaag aatggtacaa gaaaagatca tccttgaaaa
    99301 aggaaagcta acaataacta gcatcatata agagctttta aggatagaac agtagaatag
    99361 gaatctatga gcaagtcctc tcgtataaat aaaataggag ggaagtagca gcatattttt
    99421 cataaagtgg ctctctttct tcatttttac tttggttgct cctatttgaa aattcgagca
    99481 cactcaaata caataaaaag aaatggcaag ggagaggatt agatgaaaaa agaagaaaaa
    99541 atcgcgattt tacaagagat catccgaatt aaatcagtga atggaaacga aggtgaagta
    99601 gcagcctatt tgaataagct tttggcaaag catgacataa caggagagat tgtttcttac
    99661 agagatggta gagataattt gattgctcgt taccaaaagg gccaatcagg aaaagtgtta
    99721 ggcctatctg ggcatatgga tgtcgtggcg gctggtgatg aatcgtcttg gacctatgca
    99781 ccctttgcag cagaaatcca tgggaaccgt ttgtacggac gtggtgctac agatatgaag
    99841 agtggcttgg cagcaatggt gattgccatg atcgaactga aagagtcagg aaaaccattt
    99901 aacggaacag tcaaattatt agccactgtc ggagaagaag tgggtgaact aggtggggaa
    99961 cagctcacga aagcaggcta tgtagatgac ttagatgcct tgatcatcgg agagccaacc
   100021 aattacagtt tgatgtatac gcacatggga tcaatcaact atacagtcac ctcgcacgga
   100081 aaagaagcgc atagctctat gccagaccaa ggttataatg caatcaatca cctaaatgaa
   100141 ttcattacga aagcaaatgc tgaaatgaat cacctcgctg aaacaatcga gaatcctgtc
   100201 ctaggaaaaa caatccataa tgttacattg atcagtggcg ggaatcaagt caacagcatc
   100261 ccaagccatg cgcagctcca agggaacatt cgatcgattc cagaataccc aaatgataaa
   100321 atcattgcat tgctgcaatc aatcgtcaat gaattgaatc aagagacaga ctatcatttg
   100381 gaattgatga tcgattacaa caaaatacct gtcaaagctg atccagattc tcctttgatt
   100441 cattgcattc agcaacaatt cagccagcca ttacctttag ttggagcggc agcaacaacg
   100501 gatgcagccg aatttacgaa agctaaccat tccttcgact ttgtcgtctt tgggcctggt
   100561 gtcgttactt tgcctcatca agtagatgag tatgttgaaa tcgataatta tttggatatg
   100621 atcgaaaaat atcaaggaat tattttatcc tacttagcgt aagatttttt taataaatga
   100681 aaaaacagag ccagattagc attattt
//
LOCUS       DASHHB010000003        92788 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.003, whole genome shotgun sequence.
ACCESSION   DASHHB010000003 DASHHB010000000
VERSION     DASHHB010000003.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 92788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 92788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..92788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.003"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(30..1421)
                     /gene="sufB"
                     /locus_tag="U2K71_000212"
     CDS             complement(30..1421)
                     /gene="sufB"
                     /locus_tag="U2K71_000212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HEM7314333.1"
                     /translation="MGVPELEEYKFGFHDDVEPVFSTGEGLTEEVIREMSRIKCEPEW
                     MLEFRLKSLETFNKMPMQTWGPDLSDIDFDAIKYYQKPSDKPARDWEDVPEKIKDTFE
                     KIGIPEAERAYLAGASAQYESEVVYHNMKDEFEKLGIVFTDTDSALKEYPELFKEYFS
                     KLVPPTDNKLAALNSAVWSGGTFIYVPKGVKVDVPLQTYFRINAENTGQFERTLIIVD
                     EGASIHYVEGCTAPTYSSNSLHAAIVEIFTRKDAYCRYTTIQNWSDNVYNLVTKRAKA
                     YEGATVEWIDGNLGAKTTMKYPSVYLDGKGARGTMLSIAFAGANQIQDTGAKMIHNAP
                     NTSSSIVSKSISKDGGEVNYRGQVTFAKNSAGSISHIECDTIIMDEWSKSDTIPFNEI
                     HNSQVSLEHEAKVSKISEEQLYYLMSRGLTESEATEMIVMGFVEPFTKELPMEYAVEL
                     NRLISYEMEGSVG"
     gene            complement(1426..1896)
                     /locus_tag="U2K71_000213"
     CDS             complement(1426..1896)
                     /locus_tag="U2K71_000213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system NifU family Fe-S cluster assembly
                     protein"
                     /protein_id="HEM7314334.1"
                     /translation="MGLSKLDNLYRQVILDHSSHPHHRGSLTDSTSKIELNNPTCGDV
                     IELQVDVKDGIIKNIAFDGSGCSISTASASMMTDAVIGKSIEEAEHLTVDFLQLVQGK
                     EVKDPDQLGDAAMLGGVAKFPARIKCATLAWKALGKAMENDGTAAFHHYHKEEE"
     gene            complement(1883..3118)
                     /locus_tag="U2K71_000214"
     CDS             complement(1883..3118)
                     /locus_tag="U2K71_000214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM7314335.1"
                     /translation="MIDAKKLRNDFPILFQDVNDEPLVYLDNAATTQKPKQVLQALAY
                     YYEHDNANVHRGVHTLAERATHDYEQAREKVRAFIHAKETAEVLFTRGTTTSLNWVAR
                     TFGDRYIKAGDEIVLSYMEHHSNVIPWQQLAKRTGAVLKYIELTEEGFLDMEDAAKKI
                     TDKTKIVSIAHVSNVLGVFNPVQELAELVHENGGVIVVDGAQSAPHMAIDVQALDCDF
                     FAFSGHKMCGPTGIGVLYGKRKWLEEMEPVEFGGEMIDFVELYDSTWKELPWKFEAGT
                     PNIAGAIALGHAIDYLQKLGMDNIHRYEEELAAYVLPKLQAIDGLTVYGPQDPNFRTG
                     VVAFNLDGLHPHDVATALDMEGVAVRAGHHCAQPLLKYLEVSATARASFYFYNTTQDA
                     DRLVEAIMATKEFFQHGTI"
     gene            complement(3115..4401)
                     /gene="sufD"
                     /locus_tag="U2K71_000215"
     CDS             complement(3115..4401)
                     /gene="sufD"
                     /locus_tag="U2K71_000215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HEM7314336.1"
                     /translation="MKEKNWLDYLDAVNDFSLSKGEPDWMRTFRQDALAKADELPLPH
                     IDRVKFHRWSLFDVKETQTISETGTIPAFDAMKDNPVLVQQGSWTIFEQLPVELAEKG
                     VIFTDLFTAMIEYPELVQEYYMKKAVNMNEDQLTALHVAFMNSGIFLYVPKNVVIDEP
                     LESLFIQDGASDEHFFKHVLIVADEHSEFSYLERFQTTKEQVAKSSGNIIVEVIAKAG
                     SKIKYSAVDQLGENITSYMNRRGHILRDASVDWAIGVMNDGHVIADFDSDLAGEGAHA
                     EVKIVAISSGRQIQGIDTRVTNKAPHTIGHILQHGVIREKGTLTFNGIGHILKGAKGA
                     DAQQESRVLMLSDKARGDANPILLIDENEVTAGHAASVGRVDPEEMYYLMSRGLHKEE
                     AERLVIRGFLGSVLTAIPVEQVRKELVEVIEGKLNG"
     gene            complement(4414..5187)
                     /gene="sufC"
                     /locus_tag="U2K71_000216"
     CDS             complement(4414..5187)
                     /gene="sufC"
                     /locus_tag="U2K71_000216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HEM7314337.1"
                     /translation="MSVLEIKNLHVSIEEKEILKGVNLTMKTGEIHAIMGPNGTGKST
                     LSAAIMGNPNYEVTEGEILFDGKNVLDLEVDERARLGLFLAMQYPSEIPGITNAEFMR
                     AAINAKRDENDKISVMAFLKKLDEKMALLNMPEEMAERYLNEGFSGGEKKRNEILQLL
                     MLEPTFAILDEIDSGLDIDALKVVAKGVNEMRGDNFGALIITHYQRLLNYITPDVVHI
                     MMEGRVVMTGNADLAKRLEAEGYAGISKELGIDYKEEEA"
     gene            complement(5384..6232)
                     /locus_tag="U2K71_000217"
     CDS             complement(5384..6232)
                     /locus_tag="U2K71_000217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEM7314338.1"
                     /translation="MKKKIFGFAAALLLTVGLAACGNNSDSKDSANKADDTTLKVGAS
                     PTPHAEILEHVKPLLKDEGIDLEIVKFDDYVLPNQSLEEGDIDANYFQHIPYLNKEIK
                     EKGYDFVNAGAVHIEPMGLYSKKVKDVSELKDGATIITSNSESDWGRIITILQDAGLV
                     KVKDGVDLETATFDDIEENPKNLKFNHTIDPSLLASTYQNDEGDLVAINANFAYGAGL
                     NPLEDAVLLEKDNSPYVNIIATRKGDEDSDKIKKLIEVLHRKEVQEWITDKWGGSVKP
                     VKADAK"
     gene            complement(6256..6942)
                     /locus_tag="U2K71_000218"
     CDS             complement(6256..6942)
                     /locus_tag="U2K71_000218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314339.1"
                     /translation="MDKSFSETYLDFKQINPETMNQAILDTLYMTLISMVLVTIIGLV
                     LGLILYSIGRKNKPYARIIYGIVSIISNIFRSTPFMILMVLIIPFTKAIVGTMLGAQA
                     AIPALVLSAAPFYARLVEIAFREVSPGVLEAADAMGASYWEIIWKVLIPESIPALISG
                     LTVTTISMIGFTAMAGAIGAGGLGGLAWQEGYQRGNLTVTFVATLIILLIVFIVQGIG
                     DFLTKKTDKR"
     gene            complement(6935..7969)
                     /locus_tag="U2K71_000219"
     CDS             complement(6935..7969)
                     /locus_tag="U2K71_000219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7314340.1"
                     /translation="MSLIQLTNVKKTFNGKNGNIQAVNDVSLNVEKGDIYGIVGYSGA
                     GKSTLVRLLNGLELPTSGEVIVNDQDITKLKNKELRTFRKKIGMIFQHFNLLWSRTVL
                     ENIQLPLELAGVPKSKRKERAEELLRLVGLEGRGQAYPSQLSGGQKQRVGIARALAND
                     PEILLCDEATSALDPQTTEEVLDLLLAINKKLNLTIVLITHEMNVIRKICNKVAVMEL
                     GKVVEEGDVLTVFRQPKKEVTKRFVQQDIEPEADSTELLAELIKENPSGRLATLTFSE
                     RNANEPVISQAIRRYNIDVNVVYGKIKQVKEGSFGSLTVLMTGETKELDQAEVFIKEQ
                     GVGIEVIHRG"
     misc_binding    complement(8032..8288)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(8364..8702)
                     /locus_tag="U2K71_000220"
     CDS             complement(8364..8702)
                     /locus_tag="U2K71_000220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein H"
                     /protein_id="HEM7314341.1"
                     /translation="MAGKHLKKKDNLWILFNGKEYCVGLTNEAQEELGDITFANLPKA
                     GQSLKKDEPLIEVEAEKAVNEFASPLTGTVSSVNDKIGEDINILNDSDEMNAWILSFK
                     DVDPKEFDEL"
     gene            complement(8704..9060)
                     /locus_tag="U2K71_000221"
     CDS             complement(8704..9060)
                     /locus_tag="U2K71_000221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase family protein"
                     /protein_id="HEM7314342.1"
                     /translation="MYTFYWYPKCSTCKKAKAWLDQHGVSYQTVDMIKEVPKASTIET
                     WLETSQVPIRRLFNTSGMKYRELGLKDQVGEFTASKASQVLSTDGMLIKRPLILREDQ
                     LVAIGFNEKIYEGELI"
     gene            complement(9066..10253)
                     /locus_tag="U2K71_000222"
     CDS             complement(9066..10253)
                     /locus_tag="U2K71_000222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEM7314343.1"
                     /translation="MEKKLKASNDNRIDYGVILPVFLLCLIGLLSLYVALSHDPNNPA
                     VLKGVGMQVVWYLVGAAAIVVIMHLNSKWIWKLTPYLYGLGLVVMGLLLKFYDQNLAA
                     STGSKNWFRFGAFTFQPAELMKIAYILMMALIVTKHNTQVKERTMKSDFWLIGKLLIV
                     TIPVLALIMAQDDFGTMLVFLAIFGGIFLMSGISWRIIAPVVILAVVVGAGTIFLVTT
                     EGGRDLLYKVGFKSYQFARIDSWLDPFHDTSGMSYQPAQGLLAIGTGGLFGKGFNVSN
                     IYVPVRESDMIFTVIGENFGFIGGAFVIFLYFILIYRMIRVCFDTNNEFYAYIASGLI
                     MMLLFHVFENIGANIGLLPLTGIPLPFISQGGSSILGNMIGIGLILSMRYQNEAPARS
                     SRR"
     gene            complement(10444..11289)
                     /locus_tag="U2K71_000223"
     CDS             complement(10444..11289)
                     /locus_tag="U2K71_000223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM7314344.1"
                     /translation="MFKLLTTFRAVYETRNFSRAAEVLFLSQPAVSNQIKQLERELNI
                     DLFQRNGRKEMLPTKQADLLYERTLVLLEEWEETQQKLLNEQNEEEVCRIVASHTFAV
                     YLLPQLMKHLIKRFPKVHFEIEITNSHESLEQVAKHESDFGFIEKPLETSGVQRYSLM
                     EDQLVIAGDPKSKLWLVREANSGVFHYTQRYFEEMNIQDKKMIVKSNDVIVALLKEGI
                     GRSILSKRSVPENVPMTPLNFHRYFYFIQRTNLVSDQLLEIAEEILRYNESTETSHSE
                     ENLSW"
     gene            11523..12488
                     /locus_tag="U2K71_000224"
     CDS             11523..12488
                     /locus_tag="U2K71_000224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TDT family transporter"
                     /protein_id="HEM7314345.1"
                     /translation="MKNFLKIIPIPICGLILGLASLGNLFKDYHHVALGNLTGGISMI
                     LMIFIFVKLFILFEHTKQTLHDPIIASVSPTFTMSLMVICTYFVSYESIAPIVKFIWL
                     CAVIFQVILVFYFNYHHVIKADLSIEAIYPSWFIIYVGFGVITVTAGNFFPLIGKIFF
                     WISLICYAFLLPIIIKRVFFVRNMAHPTRPLITIVAAPGSLCLTGYLKNFAEPNFYLV
                     VALFALSQVLYFVVLSMLPRLFKLRFYPSYAAFTFPLVISATAIFTTVHYFNSLGIYS
                     SVLDFLKVAECLFASVVVFYVLAHYVRFLIKEHSKHGKFVSKKIA"
     gene            12865..13908
                     /locus_tag="U2K71_000225"
     CDS             12865..13908
                     /locus_tag="U2K71_000225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HEM7314346.1"
                     /translation="MKSLSHPIKSVKDSAVREILPGLLLSFAIAAFSRLLAIYLPQLG
                     GATIAILLGILLGNTFFKQPVLAIGTKFAESKLLEFSVFLLGATVTAQTIAQIGVKGI
                     VFVLLQMSVTIIGAYLIGKKLQFSDTSSLMMAGGNAVCGSSAIASIAPSIHAKEEEKG
                     QIITLVNLLGTVMMLTLPFLGLQLFGGNLIEKSALLGGTLQSVGQVVAGASLLDNSVV
                     EFAMLFKILRIMMLVVVVYLFEKFMHKKQPALFETPTDTQKKRKLPLPWYVLGFVIFC
                     LVNSLFSLPVVIDQSAHFISSWFEITALAAIGLRLDFAKFMKEGTRFLSYGLAVGLLQ
                     TVMALLFIWLIKV"
     gene            complement(13970..14434)
                     /locus_tag="U2K71_000226"
     CDS             complement(13970..14434)
                     /locus_tag="U2K71_000226"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HEM7314347.1"
                     /translation="MKIHTPIGFLWLDADESGLTAVSFQPISESESANQRILTRTQEQ
                     FEEYFAGKRKEFDVPLSIKRGTPFQQNVWKALSTIPYGEIRTYKEVAVAVDSPKAVRA
                     IGQANRTNPLPIVIPCHRVIGQNGKLTGYMGNAIDGVEIKRQLLLLEGYLQN"
     gene            14680..15627
                     /locus_tag="U2K71_000227"
     CDS             14680..15627
                     /locus_tag="U2K71_000227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM7314348.1"
                     /translation="MKVSIVVPCFNEEKTVPLFFDEVEKFKGAHEFEYLFIDDGSKDG
                     TLAAIKKLASAYPYVRFVSFSRNFGKEAALYAGLQASTGDLVTVMDVDLQDPPELLPE
                     MINRITTSDIDCVGTRRSTREGEPAIRSFFAKKFYQLINKISDTEMVDGARDFRLMTR
                     QMVDAVLELTEYNRFSKGLFSWVGFKTEYISYENRERVAGETSWSFWKLFNYSIDGIV
                     NFSDAPLTIASFIGALSCVGSGIAILFIILRALLFGDPTSGWPSMVSIFLFIGGIQLL
                     CLGIIGKYIGKIFLETKQRPVYIIRETEKVKPNELKPHQ"
     gene            complement(15838..17910)
                     /locus_tag="U2K71_000228"
     CDS             complement(15838..17910)
                     /locus_tag="U2K71_000228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317775.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEM7314349.1"
                     /translation="MKQLILAEKPSVAKDLSKVLGANQKHKNYYEGPKVIVTWALGHL
                     LGLKMPEDLNKEWQSWQMDTLPMIPKKLGIKPLPKTGHQLKAIKQLAQRKDVSEAVIA
                     TDAGREGELVARWILEYVHFNKPVKRLWISSQTDKAIKTGFKQLRPAKEYDALYESAL
                     ARAKADWLVGLNVTRALTVKYADNLSAGRVQTPTLALVRDQEKKIEQFRPQTYFTITL
                     QAGKEQAKMLQKNQFALKTQEEAQKLVQLISTSKGTVTEVQEKLKTESAPLPYDLTEI
                     QREANQRYGYSAKKTLSLVQSLYETHKIVTYPRTDSKYLTNDMKSTMKERLQAVSDFA
                     PEVKQYLKNGAIVQQTKVFQDSKVTDHHALLPTENRARYEKLSNEERKIYQMIVSRFL
                     GLFAQPHKVSQTKVTVEFGKERFIFRQNRVVQAGWKGETETETETVKWEKGMRINPDF
                     AIKKELSAPPKPLSEASLLGQMEKYSLGTPATRAEIIEKLIKSELMERTPQGLQVSPK
                     GKQLLVLVNPSLVTPDLTESWEKDLEAIAAGKKQAGTFLNGIEKETKRLVNEIKSSKQ
                     EYQDFSITQKKCPECGANLREKNTRDGKIYVCTNSDCNYRRRKDPKVSNHRCPQCHRK
                     MEIIEGKNGAYFRCKYDGTTEKLLDKKEQKKKMTKHEERRLLKKYAQAEEPEESPLAA
                     ALKAAMKK"
     gene            complement(17843..18220)
                     /locus_tag="U2K71_000229"
     CDS             complement(17843..18220)
                     /locus_tag="U2K71_000229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1033 family protein"
                     /protein_id="HEM7314350.1"
                     /translation="MYQVITMFGDNEPWWFFEDWEEDIEEEETFESFEEARQAYEKQW
                     NEIHQNYEYINAKSNFLSAFWNDGDERWCEECDDDLQQYKGLALLKNHQPITVESRKE
                     FYETTNSSGKTKRCERPKQGAWC"
     gene            complement(18349..18927)
                     /locus_tag="U2K71_000230"
     CDS             complement(18349..18927)
                     /locus_tag="U2K71_000230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7314351.1"
                     /translation="MRGNMEMKKRNLSQEKIIDCFRELAEEMDVQQITFQHLAKRLDI
                     KAPSLYNHFKNIRDVKTALTAKLLQELNDQLRRALVGKSGQEALQIYAETYRSFAFSN
                     QAVYELLISVPHTNEAVLLDGIHETNQIILQIFEAFQLTKEEKVHRSRELRSMIHGYL
                     SLRFLGYFTKEPTIDPEDSYFWMINNFIATLP"
     gene            19059..20351
                     /locus_tag="U2K71_000231"
     CDS             19059..20351
                     /locus_tag="U2K71_000231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEM7314352.1"
                     /translation="MAARIKEKLFRKRETWEQNLFVLWFGTFMAGIGFSLVMPFMSLY
                     IDTLGNYSTSQLNFWSGLTFSSTFLVTTLISPWWGKLADQKGRKLMLLRASLGMAIVI
                     SLMGCVTSVYQLVALRLLQGIFSGYISNATALVATGTPKEKSGQVLGTLATGSVTGTL
                     LGPLLGGISASAFGYRPTFFITGIILFFVFLLSLFFVHEKFVPVKKENMVSAKQIFKD
                     LKYPHVVIGMFVTTMIIQASNNSISPIISLYIRQLLHGHGNVTLISGIIASIPGIATL
                     IAAPRFGRLGDKIGSERILAIGLGFAILVYIPMAFVTNVWQLAALRFLIGISDACLLP
                     AVQALITKYSPHQAAGRIFSYNQSFQATGNVVGPMIGSSVSSAFGYRGVFLSTSLLVL
                     VNFLLVRHNTQEIHQQETAEEKASGVSIASGHSPSHRQ"
     gene            complement(20411..21529)
                     /locus_tag="U2K71_000232"
     CDS             complement(20411..21529)
                     /locus_tag="U2K71_000232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HEM7314353.1"
                     /translation="MKIANFGVEEWLNKWEKKAVYDIAQSSIEALTLDELVGLDGTSV
                     SKFFEKRKNEPLDYGWIEGSDVFKQEVASLYQTVDPENILQTNGATGANLLALYALVE
                     AGDHVVSMLPTYQQLYDIPKSLGAAVDFVHLKEEEDWQFDLEQLEALVKPETKIICLN
                     SANNPTGTLLDRKTLEKIAEIARTVDAYVLIDEVYAPLTDKGEFLSIVDVYEKGIATN
                     SLSKTYSIPGIRIGWTATGPELAEVFRKYRDYTMICGGVLSDDLAVHALKNKEKILAR
                     NKKIITENLVILKQWVANEPKVELVAPNYVSTSFIKLAIEEDDQTFCINLLEETGVLL
                     VPGSAFDLPKHARLGYCCKKETLEKGLFLLSEFLKKQA"
     gene            complement(21543..22964)
                     /gene="arcD"
                     /locus_tag="U2K71_000233"
     CDS             complement(21543..22964)
                     /gene="arcD"
                     /locus_tag="U2K71_000233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine-ornithine antiporter"
                     /protein_id="HEM7314354.1"
                     /translation="MEKKSTGISKWGLVALVVSSSIGSGVFGITSDLASSAAPGPAIL
                     SWIIVGIGILALVLSLNHLGEKRPDLDGGIFGYAEASFGKLGGFISGWGYWLSAWLGN
                     VAFATMLMSAMGEFFPIFKGGQNVPSILFASIFIWGLTLLVNNGVEGASVINTIVTIC
                     KLVPLFLFLIFVFIAFKVNIFTADFWGNVSDNLVNGTGQQGTIWLQIKGCLMVMMWVF
                     VGIEGASVLANRAKKRSEAQQASIIGLLCLLFIYILASILPYGVLSQEELATISQPAM
                     ANILKGIVGNWGAALINIGLIISIIGSWLSWTMLPAETTMLMARDGLLPKGWGKTNKK
                     NAPTYSLVVTAILTNLFLLTFLITDYAYQFAYSLCTAAILICYLLVGIYQVQFSLKEG
                     IKKQVVIGGIAVVFELMGIILAGFSYVLLCSIAYLPGFCFYWIACRETKHQITGKEKV
                     MIGLICVAALLSIGLLAVGWIKI"
     gene            complement(23210..24331)
                     /gene="mnmA"
                     /locus_tag="U2K71_000234"
     CDS             complement(23210..24331)
                     /gene="mnmA"
                     /locus_tag="U2K71_000234"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HEM7314355.1"
                     /translation="MTDNSKTRVVVGMSGGVDSSVTALLLKEQGYDVIGIFMKNWDDT
                     DENGVCTATEDYKDVAKVAAQIGIPYYSVNFEKEYWDRVFEYFLAEYRAGRTPNPDVM
                     CNKEIKFKAFLDYAMDLGADYVATGHYAQVTRDENGVVHMLRGVDNNKDQTYFLSQLS
                     QEQLSKTMFPLGGMEKSEVRAIAERAGLATAKKKDSTGICFIGEKNFKQFLSNYLPAK
                     KGNMVTLDGEVKGQHAGLMYYTIGQRQGLGIGGGGDSQEPWFVVGKDLATNTLYVGQG
                     FHHPALYATSLDASEIHFTTNEPMPKEFKCTAKFRYRQQDVPVTVRLLDDNRAEVVFD
                     EPVRAITPGQAVVFYDGMECLGGGLIDHAYQETKVLQYV"
     gene            24735..25334
                     /locus_tag="U2K71_000235"
     CDS             24735..25334
                     /locus_tag="U2K71_000235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HEM7314356.1"
                     /translation="MNYFISDMHFFHERLLGKNDFAPRLFANVDEMNQQLITSWNAVI
                     KETDHVYHLGDLAMHPQYEKANAEILELVTQLQGTIHLIKGNHDSRAFFRYLEKNDPR
                     LTDGSPKFYFYDVGAIVKFDHQQYYLTHYPMLLGPNDTIRNLHGHIHHYSVPIENDVN
                     VGVDAPERELLKEKLPFGTPLSEQQIKEIYEAKRKYDRS"
     gene            complement(25472..26758)
                     /gene="efmA"
                     /locus_tag="U2K71_000236"
     CDS             complement(25472..26758)
                     /gene="efmA"
                     /locus_tag="U2K71_000236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter EfmA"
                     /protein_id="HEM7314357.1"
                     /translation="MENEQSVVLTNWKRNYLFFLSGQFLSGITSMVVQYAIIWYLTRE
                     TGSATILSFATLLGMIPMVLLSPFVGPLVDRWDKKALLIVTDIIVAIFALILAVVGTI
                     SESFPIWLVFVSLFMRSVAQTFQMPTIQSIMPTIVPSSHITRTNGQLGMVQSANFIIA
                     PALGAALFSVVPVNYLILLDVLGAVFGVGLLIFVKIPKVSPEILEVPLTIFKDAKFGL
                     QQLMDNKGLWYITINGAFVMLLFMPAISLYPLMTLDYFGGSVGQAGAVEVVYAVGMLL
                     GGALISFIGTWKDRMKPIIIAYIIMGLTIGASGLVPNDSQGFLYFLILNAGAGCATPY
                     FNTLLMAMIQQSYESNVLGRVLGNFNSLMNLAGPIGLLFAGPLADRLGVEKMFLFSGI
                     GILLCGIVLFLTSAARNYDKELQKKLVKEHHEQKDE"
     gene            complement(27029..27373)
                     /locus_tag="U2K71_000237"
     CDS             complement(27029..27373)
                     /locus_tag="U2K71_000237"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1831 domain-containing protein"
                     /protein_id="HEM7314358.1"
                     /translation="MAFETTATVLGAPISYRLHPDAKRYTLRDNGFTETNGGNYQLIR
                     PLDATPQSKEGFKLKITVSKDIQTLKMSITTANGLKAVNIFKDPKQKMSQDKFYFLMD
                     GMISRGLFEKVE"
     gene            complement(27510..28655)
                     /locus_tag="U2K71_000238"
     CDS             complement(27510..28655)
                     /locus_tag="U2K71_000238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM7314359.1"
                     /translation="MNEVKTIYLDHAATTPMHPQVIEAMTAIMQDTFGNPSSIHGFGR
                     HAHEKLETARQIIADSLQAKPHEIIFNSGGTEGDNTAILETAFSRQKEGRHLITTAIE
                     HPAVLNTMKYLETKGFDVTYLPVDEKGNLSIEEVKKALREDTILVSVMYGNNEIGNLM
                     PIKEIGELLADHPAYFHTDAVQAYGNQTIHPEELGIDLLSISAHKINGPKGVGFLYKR
                     DGVNIPALLLGGEQEEKRRAGTENLAGIWGMAKAVELLSPEEREARNNKYKGFQQLIL
                     DTLEANEIPYQVNGDLTNKLPHVLNLRLIGEANDLLLMKLDLNGVAISTGSACTAGNI
                     EPSHVLEAMYGEDTPILKESIRISFGLGNTEDDIQYFLDQLVRAVKK"
     gene            complement(28835..29809)
                     /locus_tag="U2K71_000239"
     CDS             complement(28835..29809)
                     /locus_tag="U2K71_000239"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEM7314360.1"
                     /translation="MSNKYQDDTLKIFSLNGNRPLAEKIAKVFGTELGKSVVKQFSDG
                     EISINIEESIRGDHVYIVQSTNAPVNDYYMELLIMIDAMKRASAKTINVVLPYYGYAR
                     QDRTAKPHEPITAKLIANLIEEAGATRVLTLDLHTVQVQGFFDIPVDNLFTMPLFAHY
                     YRQLGLTGDDIVVVSPKNSGVQRARSLSEYLNSTLAIVDHADDASTEGGYVIGDVQGK
                     TCIMVDDILNTGATLARAANVLKENGAKEVYACASHGLLSDHAKSILDNAPIKDICIT
                     DSVFTEENRHPENLTIVTCSELMGEAVKRIHENTPMSPLFRLEEKEFE"
     gene            complement(29889..30764)
                     /locus_tag="U2K71_000240"
     CDS             complement(29889..30764)
                     /locus_tag="U2K71_000240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide dehydrogenase"
                     /protein_id="HEM7314361.1"
                     /translation="MKKRRRSQLNQVVDKPLYFKVDKKIKKLASTQQLQSRKSKLLFL
                     ALVFEDQSYVIIDQSGHPVEYSPAKYTYQEGLSCKKWKLINEEPIELSRWINRKEDIP
                     VLIEEKRSGKELANCWVGLPEERFLRYKKWATPSGYLCGTYAAAVLLAYYQDYRKGWM
                     LPNEIRKKNTADSLVLTKALRSQIQPLGLPTIPFQVSTGISSFLKKNGNHERARATLL
                     GSWQRATKRIREGKPVMIGILKVLGSTYGNHWVTAYAYFETETGERYYKVHDNWGDYH
                     KVIPASWSNGTVSLP"
     gene            30894..32801
                     /locus_tag="U2K71_000241"
     CDS             30894..32801
                     /locus_tag="U2K71_000241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M13 family peptidase"
                     /protein_id="HEM7314362.1"
                     /translation="MNKERLKQDFYEAVNEEWQKTAKIPADKPATGGFQDLVDGIDQL
                     LMGETDDLLAHPEKVSSEKMKHFLAYYQLANDYDRRNELHAQPLQPIIEKVESLQSFD
                     ELNKQFPEWLLDTLPLPFNLDVDADMKNAQTNAFFAYPPSLFLPDKTYYASDHPNGAQ
                     LIGVFFDMAVQLLELAGKEKSQAEAITEQAVQFDKLIAPHVKSAEENADYSKMYNPQS
                     FDTFIKYTDKLDLKKLVTGLIGTTPDKVIVTDPVYFDHLNDILTEAHFPLFKSWMIIK
                     TVRSLSGYLSEEFRQVSGIYSRVLSGTDEAMPQKKAAYYLASGQFDQVVGDYYGKKYF
                     GEAAKKDVEQMVKKMIAVYKKRLEENDWLGDATKEKAVIKLNNLGIQVGYPDKIPALF
                     DQFKTVPADQGGTLLSNTLVFSKLTLKDRFSKWNKPVDRSEWEMSADTVNAYYHPFRN
                     VIVFPAAILQAPFYSLEQSSSANYGGIGAVIAHEISHAFDNNGALFDEYGNLNNWWTE
                     EDLTQFQNKAQAMIEEFDGLPFADGKVNGKLTVSENIADAGGLSCALEAAKTESDLSI
                     EEFFVNWATIWRTKAKKEYQQLLLQIDVHAPAKLRANVQPKNLDEFYETFGITEQDAM
                     YLAPEKRVRIW"
     gene            complement(33342..36620)
                     /locus_tag="U2K71_000242"
     CDS             complement(33342..36620)
                     /locus_tag="U2K71_000242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1565 domain-containing protein"
                     /protein_id="HEM7314363.1"
                     /translation="MLKKRIKYSALMMNLLMLSTPVLISVVEVAANEQQSVNEENAHV
                     NIEGMSTMEEIPRTSEDMDTEEGNDPMMKESENEKTKKQSEEANEEWKGVVFGESTSA
                     SRYAIEPLEDGVRLSSTNNGGKFQSSGSDGMTYYYQAIPKDINFRMRATIEIESWTYT
                     NAQEGFALMVRDAVPKNHLFGQAFYSNSFAILGSRIEYVWDSDRQEVVNTSGSKYTMR
                     LGLGTRAITGISSEDPQAAPAPGSVSVETTPLETSAGFLEKETGSYNIFGNGHGNLFP
                     ATNSITKLDVEMKKTNTGLEAYYYDPETGEEMASDIMYDWEKLYASDESVIYAGFAAA
                     RNMTIKVEGIEVAYSDPATDPPGQERPTRLIDPIYTVTSSNHSGNQDHTMSFRANADG
                     LLTIKNKATGEVLKNAKNLAITANREFDYQTKLMEGTNEFTFSFTPDKNYPPEEYVEM
                     TSYETKEILHTVDYRKPKYSTVYVTPEGSDAGNGSRQNPLSLTQALRYAYAGQTIVMA
                     PGTYSFERGLTIPKGVNGTRENPIQLIAEKNPENKRPVLDFKGTGNGMTLFSHYWGLR
                     GFDITNSAAMQKGLQISGNHNLIEEIHAYRNGNTGIQISGSSNDPFEAWPAHNLVKNC
                     TSFENADPGFEDADGFAAKLTIGEGNVFDGCIAYHNADDGWDLFAKNETGSIGKVIIK
                     NSVAYRNGWVPGIEGEGNGNGFKMGGSSLTGPHELINSLSFENLAKGIDSNSGTDIIV
                     NSSTSFNNGSYNVALYTSSAGNTDYHASGILSFRTENLEMREQIRPLNQNEDQIYHSL
                     NYYWNEREKQSENTLGHTISKDWIESLNTCSKEGQQPVTRYENGSIHVHGLMQIKPGN
                     RQTEKERVDGLPLSVGAIFDGETSPSSEYPEYAIVQEPNQTKPNEQNNPIIIQEANGV
                     AENAEGNIIELKPFTVFVSTFTPNQGTPVFEDMRYFPEKIQIVETQSSAQFSNHTAIF
                     SAPSKGEYTLEVTYRSEIFDGEKWSAHTESVTQQKAITVRETADVPPGNDKPERENPG
                     NESPNGKPNGKPSHPDRTAKESEKNGTQGLPKTGEFNNPLLALAGGILLIGVVVYVMK
                     QRKK"
     gene            complement(36891..37436)
                     /locus_tag="U2K71_000243"
     CDS             complement(36891..37436)
                     /locus_tag="U2K71_000243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM7314364.1"
                     /translation="MNALISIDYTYDFVAEDGKLTTGTAGQVIEKKLTAHTKKFIDQK
                     DFVVFAIDAHDPKDSFHPENRLFPPHNVIGTGGRELYGSLKSLYQDYEQEENVYWIDK
                     RHYSAFSGTDLDIRLRERQITDIYLTGVCTDICVLHTAVDAYNLGYKLHIFKDSVASF
                     DPVGHEWALRHFESTLGAEIL"
     gene            complement(37448..38140)
                     /locus_tag="U2K71_000244"
     CDS             complement(37448..38140)
                     /locus_tag="U2K71_000244"
                     /EC_number="3.2.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5'-methylthioadenosine/adenosylhomocysteine
                     nucleosidase"
                     /protein_id="HEM7314365.1"
                     /translation="MKIGIIGAMEEEVKILRENLSEPLSWERAGALFISGSLGNHEVI
                     VVRSGIGKVLASITTSLLIQQYGVNMVINTGSAGGIGEGLQVGDIVISDKVAYFDADA
                     TGFGYNPGQLPGMPLYYEASTYLRSEMVKAAKATNLNAKEGLIVTGDTFVDSPDKIKE
                     ILTNFPEALACEMEGAAVGQTARQFNIPFLIVRAMSDTADHSATQSFDEFIEDAGKRS
                     AEMVIEFVKHLV"
     gene            complement(38159..38443)
                     /locus_tag="U2K71_000245"
     CDS             complement(38159..38443)
                     /locus_tag="U2K71_000245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314366.1"
                     /translation="MAKGPLITRSELRKRQQAQASESLKKQRKAETAYQQEEKKIASF
                     YRKESKKNKPITKTRISEREKTTKWNSFLMKSLIIVILMLCVVFLAIAFI"
     gene            complement(38456..39019)
                     /locus_tag="U2K71_000246"
     CDS             complement(38456..39019)
                     /locus_tag="U2K71_000246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM7314367.1"
                     /translation="MLNNEQFEEKTISRKEIYSGKIIDVAVDEVRLPDGGTSKRELVF
                     HPGGVGIIAFDEQDRLLLVKQFRKPLEKVILEIPAGKIDPGEGQNPEMTAARELEEET
                     GYRAKSLSHLTSMYLSPGFANEVLHIYHAQGVEKVENPLAQDEDEVLELYHLTLEEAQ
                     QAMKDQLICDAKTIYAIQYWELLTKGK"
     gene            39247..39909
                     /locus_tag="U2K71_000247"
     CDS             39247..39909
                     /locus_tag="U2K71_000247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-bromo-4-chloroindolyl phosphate hydrolysis
                     family protein"
                     /protein_id="HEM7314368.1"
                     /translation="MIKRNWPWLLALVLLFLFVRNNSFSFVLGLLLIGVVLVLWGLFG
                     SRRKKGKQEEIPALTDELETHYEKSGMTANEISFFRQTMNQTKNEINQLQMNMQQTAK
                     LKAIDLRHDPVKAAKALFKELVKEPNRLHEASQFLYTHLPNLVDLTNKYNEINDHEIK
                     NKQTYEKLEESAQIIDQLSALIAQDYQKFVAEDLDDIDVEISVAKQSLKRDNKYEPEQ
                     KD"
     gene            40033..41229
                     /locus_tag="U2K71_000248"
     CDS             40033..41229
                     /locus_tag="U2K71_000248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toxic anion resistance protein"
                     /protein_id="HEM7314369.1"
                     /translation="MDHKPTETKDVTPVNDTLEDLLNNPFSTPTDTLTTSQQAEIDQL
                     QEQQTAARLIDKLPAERQEQAKQLAAKIDANDAQSVISYGSAAQAKLSEFSQSMLNHV
                     QAQDIGPVGDSLTELMYRLQEANPDELRAGEGNIFQRVFGKVKQSIYEVTAKYQKIGA
                     QIDKISVKLDKEKDGLLKDNLMLEQLYQKNKDYFDALNIYIAAGELKMEELQTTIIPE
                     AMKRAEETGDQMDVQIANDYTQFLDRLDKRTHDLRLARQITIQQAPQIRLIQNTNQAL
                     AEKIQASIATAIPLWKNQVVIALTLLRQKDAVTAQRQVSETTNDLLKKNSEMLKISAI
                     ETAKENERGIVDIETLQTTQNDLIETIQETLRIQKEGKEKRRHAEVELGHMEEDLKQK
                     LLDLTQ"
     gene            complement(41330..42706)
                     /locus_tag="U2K71_000249"
     CDS             complement(41330..42706)
                     /locus_tag="U2K71_000249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7314370.1"
                     /translation="MDLFRKKEINLNHTSEMKKELKTSDLIMLGIGAIIGTGIFVVTG
                     VAANQNAGPALSLSFVLAAIVVILSGLSFAEFASRVPVIGGPYAYLYVVFGEFAAWLT
                     GWLLIGEFLLAVSSVASGWSGYMQGFLKSLGAELPQALTGGYNPENGTYIDLIAVLVV
                     IFVTYIVSLEAKKALRLNNAMVYVKFGIIALFIIVGIFFVKPDNWQPFMPFGFSGVLD
                     GAALVFFAFLGFDAVAMAAEEVKNPQKDVPRGIIGSILIATVLYIIVTLILTGIVPYT
                     ELGVNDPVAFAMRYVGHGTVGAVIAVGAILTLLTVTISMMYSLARLLFAVSKDGLLPK
                     FMTEIDQKHRTPKKATYAAGVAAVFFAGFFPLNVLAELTNIMALAYLMLVSLGLLKLR
                     KMFGKPKAGEFKVPFVPLLPIVSILTCIVLMLRLQTVTWIVFGIVMVIGLIIYFGYGY
                     GHSKMNEK"
     gene            complement(42743..43609)
                     /locus_tag="U2K71_000250"
     CDS             complement(42743..43609)
                     /locus_tag="U2K71_000250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose transporter GlcU"
                     /protein_id="HEM7314371.1"
                     /translation="MEILIALVPMLAWGSIGLVSGKIGGDANQQTLGMTIGAFLFSLV
                     VFFVVSPVITPWIFLIGFLSGLAWSVGQNGQFHGMKYMGVSVGLPLSTGFQLILNTIA
                     GAVFFHEWTQTKDYVYGIIALALLVSGAYLTARQDDEGKIDTDNKMLDFGKGFRALIF
                     STIGYGVYTIIVNWANLDAMSIILPQSIGMILGASFFAFRKVKVDQFVWKNMICGLLW
                     GLGNICMLLTVKSLGLAVGFSLSQMGIIISTLGGIFILGERKTKKELIYVIVGCLLVI
                     LGGILLGYMKTV"
     gene            complement(43698..44723)
                     /locus_tag="U2K71_000251"
     CDS             complement(43698..44723)
                     /locus_tag="U2K71_000251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM7314372.1"
                     /translation="MNFFKYLKDQWTLILGWLFFIALTIFVMWLAPNIKVDLSTIAYL
                     ALIEGVFLIFYLLGGYLSKRRWWGKLADIQKTSPLQNYLSGGRTEEEKLVEEYINQLI
                     REHQEVMQSAISNQQDQKDYIDSWVHEIKVPLSASKLLVRSIEFDIDDQKYTLLENEL
                     SKIDEYVEQVLYYARLDSFSRDYLIQEYGLKGIIQPVIRSQANYFIQKNIRYEIVGED
                     HTVLTDAKWIAFIFNQLLSNAIKYTPDHGNIIVSIEKEAQGVSLSVKDSGIGIPAEDL
                     KRIFDKGFTGRNGRLSKTHSTGLGLYLAKNLAEKLGIHLTAESTEGKGTTMTLFFPIL
                     NFYNEKR"
     gene            complement(44741..45415)
                     /locus_tag="U2K71_000252"
     CDS             complement(44741..45415)
                     /locus_tag="U2K71_000252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7314373.1"
                     /translation="MAKIMVVEDEEIIRQLIMEELEKWQFETFGTTDFNQVFSDFERE
                     EPQLVLLDINLPVLDGYYWCQKIREVSKVPIIFISSRNTNMDMIMAMNMGADDFVTKH
                     FQIDVLIAKINALLRRSYNYTELSSEMMSHNGITLNVDNGSMEINGEIIDLSKNEYRL
                     LFILMKHNGKILSREKLLRALWDDERFVDDNTLTVNINRLRRKIEQAGIQGYIETKVG
                     QGYIVP"
     gene            complement(45599..46492)
                     /locus_tag="U2K71_000253"
     CDS             complement(45599..46492)
                     /locus_tag="U2K71_000253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM7314374.1"
                     /translation="MKKKIGNRPQTQISLLLQLFMAVMFLAGAAVFTYPFLADALSNY
                     LDQRRIENYQKQLERDKEEKHEQQLAVHEKKNQTLAHTSVIPGMGQVKDPFEQAVRNV
                     QNPGKEYYEQHMIGAIYIPKINVSLPLFDETNDLLLDRGATVLQGTSFPTGGESTHSV
                     ITAHSGIAEKKLFTDLEEMEQDDRFYLEVYGRMLAYEVVEKIVVLPTKTNTLAIREKQ
                     DLVTLITCTPYTVNTHRLLVTGRRVPFTEEVSSKIEQTKKYHLYRLLILLLGILLVLT
                     LFGYWCYRKFKRQKQRKKNNR"
     gene            complement(46566..48263)
                     /locus_tag="U2K71_000254"
     CDS             complement(46566..48263)
                     /locus_tag="U2K71_000254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM7314375.1"
                     /translation="MNKKMIVTGMSVLLLVTAGIGIFGNGKLVKAEEVAQKPSEVTIT
                     LHKKGFSSVPEERPNSGLVSTDFGEENIPGVDFDLFDVTEVYYDLIRDNPLTPEREDG
                     LNSAEAIEWIQKRHTESWFLKYRLTSIDKQTTNEAGEAVFSTVQVTEEAPSSRDKVYL
                     FLETYSPAHISRIASPMVVMMPVMMPDMVDGVWDGSTWKDTYNTDVHLYPKNEIREAD
                     KQMNVEESDLRQVTIINEAGEQETISYIDLERGKTASYTITAPIPYFIDSVLENGSAV
                     IKNYKITDTPTVGLTYYDQEIEVRAGETILTKGQDYIVEVVSNGFVVTILTEENGVAK
                     VDTLGRLADARGGDLTITYNLKVSTELEADDFHNNTAVIEIGRNDEFDYEEGVEPPEK
                     VTTGGRKFEKYDASSSELLKDARFELWNEDRSEYAIFYKGESPLAVYESGADRIEWAT
                     SGQATEFVADGNGYFEVQGLDYGTYQMKETMAPEGYVLPTGEAAFTEFIISYGSYNEE
                     IQIVGVENPGPERVPNMKRGSLPATGGNGLLAFLLIGISLMIGAYSWYRKSKMKSEV"
     gene            complement(48256..49569)
                     /locus_tag="U2K71_000255"
     CDS             complement(48256..49569)
                     /locus_tag="U2K71_000255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HEM7314376.1"
                     /translation="MKKLLCLMLQLLVLLPLAAVLLPHAMSAEESGEDVVEFVLHKRM
                     IRDIDYNDQFEYHENDGLAISEEAGETADIISQTVPLNGATFAIYDMTDYYHEKKAAE
                     NMTPKEFVQQIAETNRNNIRQLITEERLVQIGTSVTTGKDTVGGLGDGIARIKAPKKN
                     QDRDAVYLIFEESIDPEVGLDVDIDQTAIPIAAILPIYHPTESTEELQEIHIYPKNVG
                     YLRDPYFFKYGRQRGTTEKGKPLEGAVFALYQMIEGGKYYLDMAPANDLKNQWIKPAD
                     NDPLNDKNVSKFISDKEGLVTTGQRFLPSGTYYFEELKGVEGYEMDEASKRIEVIIPE
                     FWVDAKGQPQYVVVNGQEMAELESGKVPVSAYESATPRVYNETVKETTDKPKEPSGPS
                     NPGQSGKWSVFPQTNEARTLISLLGILLLLGAVLLIKKERGEKNE"
     gene            complement(49569..53606)
                     /locus_tag="U2K71_000256"
     CDS             complement(49569..53606)
                     /locus_tag="U2K71_000256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEM7314377.1"
                     /translation="MVVDERIDNQELQENETDTEDSAEIQRSEENEMEKLDEPIVPSG
                     QALQRIQRQLFNGILERLGPTATQDPYEYIYPSGEVENEQHRYPKFTTNDYTKVLGNW
                     TQSNERYQGDKGSPYDGENLIDDSDYTNGGANWRNYDYATNNGSTAGEEDRNVPAQTV
                     QLWGNERNFENSYLDYNGAYIKKWVEPVLPSSTASDLHPEDATTLYNVYLDVIGGEKK
                     EISPIDIVFVLDKSASMSELTAGTNSQTKNAALIEAVNEMSKDLLSDPSLDIRIGMVN
                     FYHNSTAINNHEQISSDIFPLTNDINRLTGSENTALNRTPIGGTPLTLGLKNGYETLY
                     KDNGGENRNPEKILIVVGDGTPTFSYAPIQSSYRTSTNGAWSNWTVMEDKIAEDNDVL
                     FRNFEEFSGNTSNAGFTHPVTYASDFNRPEDEVNVHYRYGEVKEGDNKATHWVGDGSS
                     NNNTNGSPTSQEKSSAINTVAYHHWLKNKYQENPPSIFSIGLGIDGNVSGRQRLDAIG
                     RNVLKNIADLEEDGVTPRYYNANNKNDIVTALEDISSTFKRTIQQATLYDETGFNVSP
                     FGAGRSADIQFYHLDPGENGMKYQEPEIWDEERHGQAPPEVVATPTTYVGRENQAYKF
                     SPISLGEGEMVRIKYQVKLDAGAQDGNFCTVNNMAYIQNLEDYENDITTGRMYFPSSS
                     IRYEHKGRNFQVSKKGQNGEPLAGVEFALYDQNPESGEAEPIETSKTTTDGIAIFETK
                     MPILGDETGAVSDIFWIREIAGPPRYQLVDETYQFQIKKYAANEDEMLGHYELVDEYT
                     SGSRVGGPEGKDPNSYYEGEYHKLSVVQEPNIEGTEYDELYVKLEMRNEFKPIHMNIN
                     KLIEGSNMPINGAEFELYETDGNGLEIGYPVAKGLSGVNEVEEEKGRLTFYSVDEDGK
                     YVIVDGEKVIHPIGEPDSFVYKDSEETTNYAEYKIIESQNPEGFKEPDENDTWILRLY
                     DNAIGTIQLKQTGEENWQYLTYTTDEEDRLWVAFTAYNTFDYREIRVRKLDHEGHTLD
                     GAGFDIRKVGDSSFPLYRAYTGNPLEPEESKRLPGIGYFYSRAVGVEKYVDLNYDQPL
                     HLSIGEYEVSESVAPNGYRLSDQVFQFALNEEGKFIIEDTVIEDETAPLPEGYEIIDG
                     VLQVTLEDELAPIDLELLKIDSMNSRRLEGAEFSIEKQNSAGEYEIIEGGLAPDPNDS
                     SLFNASDLLEGVYRIKEVKSPDSYRKLPGYFILEISYREEPDIENDRVVPGKEAGTLK
                     VEISYYPNEEAETPDLQQELEYELLETNRIRLQLSVGNDPENPLPATGGSGRLFYILL
                     AAFFMALAGAAYGLFVRQSKKEGAR"
     gene            complement(53619..54077)
                     /locus_tag="U2K71_000257"
     CDS             complement(53619..54077)
                     /locus_tag="U2K71_000257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM7314378.1"
                     /translation="MKGLKLKLLQLVTVSLLFGPYFQSVYHMTTLDVAAESTETKEDE
                     VFLDEDYAKIEGKYIEKEETLEWHLAFEKKESANEGRIRLAIDAAAAGIGTVKNVRGT
                     GLSSYDEDGEDLKVETIDGQEWYVGKVYSKEAETGTLIFETEKIPDVNEG"
     gene            complement(54375..54893)
                     /locus_tag="U2K71_000258"
     CDS             complement(54375..54893)
                     /locus_tag="U2K71_000258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEM7314379.1"
                     /translation="MLRLIAKIDDSIINKFAKQRKTDSRKINFLKFISNISNGGFIWL
                     LLAAVLVLIPDTREYGLAILIGSLIYFFVGLLFIKKTTRRKRPYEKNRRLVLYTSKPK
                     TSSFPSGHTALAFLSVAALFELSPVAGILAFLFACLIAYSRIFLLLHYPTDILGGAIL
                     GSVIGILCAAVI"
     gene            complement(54895..55511)
                     /locus_tag="U2K71_000259"
                     /pseudo
     CDS             complement(54895..55511)
                     /locus_tag="U2K71_000259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339120.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(55508..56371)
                     /locus_tag="U2K71_000260"
     CDS             complement(55508..56371)
                     /locus_tag="U2K71_000260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM7314380.1"
                     /translation="MTANDGLFQKDQCESANKKILSYVLMTVIGIILIIPLLWMVFTS
                     LKPMEEIVRYPPTFFPEKIVWENYLDTIAAFPFWRYARNTLFITVLVVIGNVLSNSFI
                     AYGFAKLDFPGKKLMFALVLSTMMIPGFVTMIPQYVLFSKIGWVGTYLPLIVPSFFGN
                     AFNIFLMRQFYLSINNELIEAAEIDGANHLYIWSHLMLPLTKPALITIAINSFNAAWN
                     DFLGPLLYIQDQEKYTLQIGLQVFQNQATTQWNYLMAGATLVLIPTILLFFFAQRYFI
                     EGMDLTGGSKG"
     gene            complement(56358..57269)
                     /locus_tag="U2K71_000261"
     CDS             complement(56358..57269)
                     /locus_tag="U2K71_000261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7314381.1"
                     /translation="MKRLSIRKKKEARWAYLFISPFIIGFLVFMLGPMLFSVIGSFTD
                     YNLTSKMNFIGLANFKRMFVHDDLFWKSLYNTVYFVIFNVPLTTIFSVFLATVLNQKI
                     KGISVFRTAFYLPAVLSGVAVYILWMQLLSPSAGLINTFLGWFHIPGPAWLFDPQWTK
                     PALILMKIWSSGGAMLLYLATLQNVPRSLYESAEIDGAGVWGKFKSITLPLITPIIFY
                     DVITSTIGSFQIFQEAYVMTKNGTGGPANSLLFYNLHMWNKAFVAFDMGYAMAMSMIL
                     FLIVLVLTFVNLKLAPKWVYYSGGDGK"
     gene            complement(57310..58644)
                     /locus_tag="U2K71_000262"
     CDS             complement(57310..58644)
                     /locus_tag="U2K71_000262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM7314382.1"
                     /translation="MKKLLFTGLLASSALFLLGGCGSSNASDASSSAANSSEKTSDGR
                     KNIDFWSFWGSGARKEAIEEIIEDFNQSQNEIHVNYKYQPWGDIWTKSLSAITAGNAP
                     DVIVQDINSVAQRAEAQQATNLSKYVEKDTSKDFYPQLWDTVEYEGDPYAIPFNTDTQ
                     VIFYNKKIFKEAGISEQDLPTTWDELEETARKLDKKEGKEFTRIGFYPLWNLGADVWA
                     LNADNGTSWFDKDEKVKIDTKNKVEALEWIQDWQDYYGKNTINKLEAEFGSGVSDPFI
                     SGLVAMRAQNINYYSSLMENAPDDFDFGVMQLPEKEEGSGHWSWGGGFVLEVPYGAKD
                     PEASYEFIKYLSSPEVQEKFGEKSFDIMASKTANENLVNNDNLTDKGKMIYQMADENF
                     KNTVITPVPLDAPDYTTLVNEQIDQILLGTKSPKEGLADAQKAVENLVKQNQ"
     gene            58852..59682
                     /locus_tag="U2K71_000263"
     CDS             58852..59682
                     /locus_tag="U2K71_000263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese catalase family protein"
                     /protein_id="HEM7314383.1"
                     /translation="MFRHQKELQFEAKPDRPNPLIAKYLQELIGGQYGEMSVAMQYLF
                     QGWSCRGEEKYKDMLMDIATEEIGHVEMLATMVARLLEGAPVEDQEAAMQKDPTIGAI
                     ISGMNPQHAIVAGLGPRPADSVGNPWCGAYIIASGNLLADFRANLNAESQGRLQVARI
                     YEMIDDRGVKDMLSVLLARDSYHQNLWECAVKELEEKEGSILVPSTFPRDKERKDIAY
                     DFYNFSEGDQSSEGSWASGKALDGEGEYQWLKEPKIEGAAPKLEPVTPKMYGTPPFKK
                     "
     gene            complement(59926..60244)
                     /locus_tag="U2K71_000264"
                     /pseudo
     CDS             complement(59926..60244)
                     /locus_tag="U2K71_000264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028215.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(60366..60560)
                     /locus_tag="U2K71_000265"
     CDS             complement(60366..60560)
                     /locus_tag="U2K71_000265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314384.1"
                     /translation="MKTTLVLFYKKHPYFTLLINILLASVIGISVEYLINKDFIGSCF
                     YTALFLGLLEAFSIYKKSKK"
     gene            complement(61065..61250)
                     /locus_tag="U2K71_000266"
     CDS             complement(61065..61250)
                     /locus_tag="U2K71_000266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314385.1"
                     /translation="MSKNSKGFLTILLAFIGYMLVGLLKSYSNELLNFSTFINDTLVP
                     SLFFIVFFAVGYFIIKI"
     gene            complement(62100..62441)
                     /locus_tag="U2K71_000267"
     CDS             complement(62100..62441)
                     /locus_tag="U2K71_000267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEM7314386.1"
                     /translation="MSEERIYIPKKGDIVWIDFDPSVGKEIQKRRPGLVVSRYEFNRK
                     TMFSVICPITSTIKNLPTRYSLPEELDIKGQVIITQLKSLDFKERKLKKIENLPLQDM
                     AKIDQIIQYMF"
     gene            complement(62441..62671)
                     /locus_tag="U2K71_000268"
     CDS             complement(62441..62671)
                     /locus_tag="U2K71_000268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM7314387.1"
                     /translation="MLVTKSRLQGSSVVVTLPSDNGKKPSENQEYIVVYSDDGTITLV
                     PKIEDPFSGGEEAEYYEKDEWEDLTPEGREIL"
     gene            62958..63296
                     /locus_tag="U2K71_000269"
     CDS             62958..63296
                     /locus_tag="U2K71_000269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314388.1"
                     /translation="MPDYKQKAGYSFCSDKFQLGKFTKLAGEEVSTVRIKECPLQIET
                     LVQRISTRQDFAIVEREIVAIFVEEGILHDPTHINVDKWQPLIYKFREYTTANQSLTL
                     NFRFQEFKES"
     gene            complement(63501..63740)
                     /locus_tag="U2K71_000270"
     CDS             complement(63501..63740)
                     /locus_tag="U2K71_000270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEM7314389.1"
                     /translation="MFSNKEKIVYIGGFYRSHQDEKMAESIIMTTEPNRTVAPIHDRM
                     PLVLTEEQIEPWVTDISFARKIITQQMPELVMEKV"
     gene            complement(63716..64063)
                     /locus_tag="U2K71_000271"
     CDS             complement(63716..64063)
                     /locus_tag="U2K71_000271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SOS response-associated peptidase family
                     protein"
                     /protein_id="HEM7314390.1"
                     /translation="MCGRYFFDLESELLQNYYREAQTKQPKQAAELAAGEVFPSNLVV
                     TLRKEEENILTPEIMKWGFTGFKKGQLMINARAETVEEKKTFHQPFLQNRCVFPMSGM
                     KKKTNLCFQIRKK"
     gene            complement(64795..65376)
                     /locus_tag="U2K71_000272"
     CDS             complement(64795..65376)
                     /locus_tag="U2K71_000272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7314391.1"
                     /translation="MKTKEKILEATKDLIYKKGYHETSIRDILAASDTGKGQFYYYFS
                     SKKEVCLAVIHAHMDVWQTELFEDILNAGKQPEEALEEMLDWIFADHETQITYHGCPV
                     GNLIIELSTVDEEYRLLLLELVNQWVKYTKQNILALSEEKLSDEEADRQAKALIAEIQ
                     GSILLLKVTQDLSLLRQNFQLIKERLLTSQKVL"
     gene            complement(65559..66185)
                     /locus_tag="U2K71_000273"
     CDS             complement(65559..66185)
                     /locus_tag="U2K71_000273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM7314392.1"
                     /translation="MFNNKNTAVVLTDPQVEFLKPAGGGFGLTKDILEKYNTIENMIE
                     MVKQAKEKGYKLFISPHYFYPHDHKWQFGGAGEHMMLEQQMFARKSQYESVEEGSGAD
                     FIEELKPYLDENTIISSPHKIFGPESNDLALQLRKNGIDTVILGGMNGNLCSDSHMRE
                     LVESGFNVVVATDAIGAPGQEAMDAALTNFGFIADQVMTTEEVLEQLS"
     gene            complement(66207..67538)
                     /locus_tag="U2K71_000274"
     CDS             complement(66207..67538)
                     /locus_tag="U2K71_000274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-containing oxidoreductase"
                     /protein_id="HEM7314393.1"
                     /translation="MMEKYQNIVIGFGKGGKTLAKALASKGESVLVVEKSTRMYGGTC
                     INIGCIPSKSLIFNGERGIDFTEAVARKEKLTGMLRAKNYHMISDEATGTVMDGTARF
                     LSNHQIEVTNNGEKVIVEGERIFINTGSEPIILPIKGLNTSRYLIDSTQAMDQEQLPE
                     KLVIIGAGYIGLEFASMFNEYGSKVVVLDAHSEFLPREDEDIAQMILEDMTNAGIEFH
                     LGVSVDQVADQESTAAVTFTEDGQEVTIQASKVLAATGRKPSTASLGLENTDVKLTDR
                     GAIAVDEYLRTDADNIWAIGDVKGGLQFTYISLDDYRIIMDQLKGENKRTTNNRKAVP
                     YSVFITPTLSNVGLTEKQAKAQGVNYKLFKYMTSGVPKAQVLEDPKGVFKVLVDPETD
                     LILGASIYAEDSHEVINLISLAMNGKLPYTLLRDQIYSHPTMSEALNDVLK"
     gene            complement(67693..68094)
                     /locus_tag="U2K71_000275"
     CDS             complement(67693..68094)
                     /locus_tag="U2K71_000275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEM7314394.1"
                     /translation="MKKAYETAMINHGGRNGEVEAPNGSMHMKITPPGIHAEGTNPEQ
                     LFAAGYASCFNGAVQHMIKEAGMTAESTVKARVSLYNLDDGGYQIGVVLEVHIDGISE
                     EEAQKIAEDAHEFCPYSKATRGNIDVEVTIV"
     gene            complement(68180..68386)
                     /locus_tag="U2K71_000276"
     CDS             complement(68180..68386)
                     /locus_tag="U2K71_000276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314395.1"
                     /translation="MVTNMVIFLTVLVYGVNFLVYMYLKINHIKDYVEKIAIYFGTNM
                     TLLFTSSIFLFFGKIVEDGILVFE"
     gene            complement(68481..69983)
                     /locus_tag="U2K71_000277"
     CDS             complement(68481..69983)
                     /locus_tag="U2K71_000277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomannomutase/phosphoglucomutase"
                     /protein_id="HEM7314396.1"
                     /translation="MKDMKALQNGSDIRGIALPVEGYEVNLTTDMVKKIGWGLANWIK
                     KKKKSTDPNKKWTIGIGHDSRISGPALKEALIQSLKEQGIHVIDFELATTPAMFMSTQ
                     FEQFSCDAAVMLTASHLPYYYNGIKFFTSEGGAEKEDIAFILSQQQKIKSEQTGSVEK
                     ADLLTPYAQDLVTKIKKGIGTLESKPLNGWKIIVDAGNGAGGFFAEKVLNSLGADSTG
                     SQFLEPDGRFPNHIPNPDNKEAMESIKQAVLANEADLGIIFDTDVDRSAVVSASGTVI
                     NRNNLIAVLSTIVLKEHPGTRIVTNSPTSSHLQRFIEEKGGIQVRYISGYRNVINKAL
                     ELNRLGKDTQLAIETSGHAAFKENYFLDDGAYVIAKILMLLPELKRKGITLDEQIKEL
                     KQPVEVQEIRLKLFSDEPREQGNKILEEMKRLLEEEKGMNFDPENEEGIRYDLEEPYG
                     NGWFLIRLSLHEPLLVLQIENDEFGKNKLALVKLAKLFGHFSDLESLTAE"
     gene            complement(70001..70456)
                     /gene="argR"
                     /locus_tag="U2K71_000278"
     CDS             complement(70001..70456)
                     /gene="argR"
                     /locus_tag="U2K71_000278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine repressor"
                     /protein_id="HEM7314397.1"
                     /translation="MKKKQRQALIRQIITEQPIGTQEELLARLHEAGADVTQATISRD
                     IREMKLVKSQNENKIVRYTLFNQPSVSLNEERLRTAIRREVLRIQSVQFMVIVLTERN
                     GADVVTNWLDEVAYPEVVGTMAGVDTFIIICRSEEEAQRFAEKLEKMRE"
     gene            complement(70554..71171)
                     /locus_tag="U2K71_000279"
     CDS             complement(70554..71171)
                     /locus_tag="U2K71_000279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HEM7314398.1"
                     /translation="MEKIEWRKDKSYSMKKSPQILEVPKKSFFSIRGIGDPNQEDFKR
                     RVACLYAVSYAIRMSPKKNWLIPDYQMYTVYPLEGQWGLQEKYLHEPVMKKEHFSYQL
                     MIRQPDFVTEAIAQEAIQRSSKLPEDLKEQVVFKKMEEGLCAQILHVGPYAEEPATFE
                     KLEAFIAKEGYERASKEHKEIYISDPRKSAPEKMKTILRVKIEKG"
     gene            71392..72765
                     /locus_tag="U2K71_000280"
     CDS             71392..72765
                     /locus_tag="U2K71_000280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7314399.1"
                     /translation="MTKQKQLLLVPAVIATGMMSFAGVLIETAMNVTFPTLIRQFDLT
                     TAQVQWVTTIYLLMISIIVPLSTYLNRNFSLKKLFLASNLLFLAGIMIDFFSPTFMVL
                     LFGRLLQGAATGIALPLMFHIILTFAPLNRRGAMMGVGTLTTAIAPAIGPTYGGIMTS
                     HFTWNHIFLFLVPVLFLSLIIGWFAIPEMAVQKSGYLDWISVLGICLLFSGSLTFLST
                     LGTVSSWIALIIGGIGWFLFYYRSKKVEHPLIRLSILSNKTFRLFLIGFLVYQFLLLG
                     VSFVLPNFLQIVQGSSPFVAGLAMLPGAAVGALLSPFSGKMLDQYGPKKPIFAGLILS
                     LIGWLALFLLIGHASVGVLIACHVFYMIGIGLSYSNMMTTGMNALNEELQGDGNAMFN
                     TLQQFSGAVATSLVAVIINYVQHHTSHNYEVSTTLGSKAALGVLLLLLLVSFARFAYY
                     LFFAKKA"
     gene            72840..73952
                     /locus_tag="U2K71_000281"
     CDS             72840..73952
                     /locus_tag="U2K71_000281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HEM7314400.1"
                     /translation="MEKIAIIGGGIIGMTLASYLDTSKFNVALFDEGTGQATKASAGI
                     ISPWLSKRRNKQWYRLAKDGAAFFPKLIKDFQLDQTIYAQSGTLLLRPEKDLAELAVL
                     AEKRKEEAPEIGNIEWLSPEQTSQALPLLKPAVSLRISGGGRLDGKAYLEHLGQRLAQ
                     KQMEQIQAKVTLHKKSDSWLIKGPFGEYTADRVILTPGPALKPLLQELGYAADIRPQK
                     GQLLAFQTSYKMSQDWPVAMLDGEADFIPFMDGKILLGATHENDQGWDLAPTSEAYKG
                     LTKSTEQFLTDPDQIKNWPYEFRVGTRAYTSDFSPFFGVLPDDPSLLAASGLGSSGLT
                     TGPYIGYLLANFLNTGHWPTEDYQKDISTYITKNPS"
     gene            complement(74024..75661)
                     /locus_tag="U2K71_000282"
     CDS             complement(74024..75661)
                     /locus_tag="U2K71_000282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HEM7314401.1"
                     /translation="MSTKESLELNEITSGNPLLSSIRSIVETTFYGNNVHEVFDRKTA
                     YQLAKGSPGTIITDLTISHAEELDLPADVRTLVFNDGSIVGRTASARRIFEDLDKEQS
                     KYEKILREAVYQSRKRQFYHTKVIVGLSEEFSVQSHLLIPEGFENNLYSYLLNFQPAN
                     SKILETYQKSVSFDEGDLYIFCDPEWTHETFPNGLILFDALHNTAAVLGLRYFGELKK
                     ATLTLAWALGNRNGFIACHGGMKQYASNDQTYTMAVFGLSGSGKSTITLAKHTEGHSV
                     SILHDDAFVISRKNGFATALEPSYFDKTQDYPSDDPAISSFLTCQNVGVTCTSKGEKV
                     LVTGDIRNGNGRTVKSRYATPDRVDHLSEKIDAVFWIMKDDSLPPIIKINDPVLAASF
                     GATLATKRSDAENITTKRQLDELVIEPFANPFRVYPLREDYQAFRELFLKQQTACYVL
                     NTGYFDDKKIKPETTLSIVERIVQQTAEFKSFGPFEDIHYLPIPDFPVSFEDTSYKNL
                     VKQRLDIRLRFIEAQIGKDLLPDEAKESLENLIQKLN"
     gene            complement(75829..76437)
                     /locus_tag="U2K71_000283"
     CDS             complement(75829..76437)
                     /locus_tag="U2K71_000283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM7314402.1"
                     /translation="MNSFKKIVLGTTFAAGATAMFVGTTNAHADEVYTVKSGDSLSKI
                     SQKFAGDNSMIDAIAEKNSIANINRIYVGEQLTIPTSNDSSATTENKTTENTASTTET
                     ATQEHTYVAPVETVEVAPAAPAAATAPTSSSAKEWIAQKESGGSYTATNGRYIGRYQL
                     DASYLNGDYSAANQERVAEQYVTSRYGSWDAAKTFWLANGWY"
     gene            complement(76821..77972)
                     /gene="queG"
                     /locus_tag="U2K71_000284"
     CDS             complement(76821..77972)
                     /gene="queG"
                     /locus_tag="U2K71_000284"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HEM7314403.1"
                     /translation="MTLKEEIIQESKRLGIDKIGFTTAEPFDSLKESLEEQKAAGHTS
                     GFEHPNIDERLYPEMTFDQPKSIISIALAYPTRIHEEVPRDEKRGQFARASWGIDYHD
                     ILRDRLNRLIEFIQTRAEKWQKEEEWRLAPQVDTGELIDVAVAQRAGLGFIGRNGLLI
                     TEEFGSFVYLGEIVTNIVFEPDKPGEFGCGDCMRCVTACPTKALLGDGRMNAQRCLSY
                     QTQTKGMMPEEYRKKMRNVIYGCDICQLVCPYNQGKDFHFHEEMEPKVDEIYPKLKPM
                     LSMSNKDFKQQFGHLAGSWRGKKPLQRNALIALANLGDRTALPEISECLTDVRPVIRG
                     TAAWAIGRLGTKEPEKWLDVLDEILAKETEEVVINELEHAQKLLRRKLK"
     gene            complement(77991..78743)
                     /locus_tag="U2K71_000285"
     CDS             complement(77991..78743)
                     /locus_tag="U2K71_000285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314404.1"
                     /translation="MNFTRKDKYTIKEEVFRLISQQENTDKDQDLQFLEAELAKLFIA
                     NQNETTEPDEELYIGKWLFDEFQPLDENTGIGYFVFLSPVGFTPGVLVKQNDEITVKA
                     WPALFSVIKAEGVIKKIQSAYYKSQFIANSKLMNMFYNRLVEIARPGKIPQTFTYTQQ
                     KNLYEEVLYSRDLSEFSPWEEVRLHELLSTKPVSLNFFNALDNKRKLISTNYWEVFAA
                     RKKWMEEKKIADQQIQFVQLKKEKLYYGLYSR"
     gene            complement(78884..79834)
                     /locus_tag="U2K71_000286"
     CDS             complement(78884..79834)
                     /locus_tag="U2K71_000286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEM7314405.1"
                     /translation="MKTKKSLFLILAVSFLVLAGCGKQASDQADEGSKEKLSVVATNS
                     ILADMAKEVGTDQIDIHSIVPVGTDPHEYEVLPEDIKKASDADVILYNGLNLETGNSW
                     FDNLMETAKKEEGKDYFAVSKNVEPLYLTSGEEHTKADPHAWLDLSNGIKYVEEIARI
                     FSEKDAENATLYKKNAEAYVEKLKELDTQAKESFASIEENKKLLVTSEGAFKYFSRAY
                     DLPAAYIWEINTESQGTPDQMKAIIDQIRAKEVPVLFVETSVDSRSMERVAKETGLKI
                     YDKLFTDSIAKEGEQGDSYYQMMKWNIETIHEGLSQTKES"
     gene            complement(79835..80674)
                     /locus_tag="U2K71_000287"
     CDS             complement(79835..80674)
                     /locus_tag="U2K71_000287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM7314406.1"
                     /translation="MIQSFIDGLMNYQFLQYALVTSMLVGLASGVIGSFIILRGMSLM
                     GDAISHAVLPGVAISYMFGFSYIFGATAFGMLTAAVIGFVTQRSRLKNDTAIGIVFSS
                     FFALGIILISYAQSATDLYHILFGNVLAVRESDLLLTALVSGIVLIFVFFFYKELKIT
                     SFDPTMAKAYGLNTSLIHYLLMFFLTLVAVVSLQTVGTILVIAMLITPAATAYLLTNH
                     LLKMIITAAGIGMLSAVVGVFFSYSYNWPSGATIVLACTAFFILAFLFSPTKGILFQK
                     GSR"
     gene            complement(80671..81417)
                     /locus_tag="U2K71_000288"
     CDS             complement(80671..81417)
                     /locus_tag="U2K71_000288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEM7314407.1"
                     /translation="MERTAIVIQDLSAAYQGKTVLKTINLTINPQKITGIIGPNGAGK
                     STFMKSLLELIPATTGKVTFSGQSVNSVRKKIAYVEQRSELDLSFPIDVLGVVLLGTY
                     PSLRIGQRPGKKEKERARQALKKVGMEEYAKRQISELSGGQLQRVFIARALAQGAEWI
                     FLDEPFVGIDALSERKIFDILQELKNSGKTILIVHHDLHKVEEYFDEVILLNKQLIAS
                     GPVQEVFTSKNLQLAYGEIIRHLVKGGEKK"
     gene            81655..83025
                     /locus_tag="U2K71_000289"
     CDS             81655..83025
                     /locus_tag="U2K71_000289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7314408.1"
                     /translation="MEEEKLTRSLSARHIQMIALGGTIGVGLFMGASSTIRWTGPSVM
                     GAYAIAGFFLYLIMRALGEMLYVDPSTGSFANYASEYIHPVAGYLTAWSNIFQYIVVG
                     ISEVIAVGSYMNYWWPDLPAIIPGIIVVLFLMLANLISVKAFGELEFWFSIIKVITII
                     LMIIAGLGVILFGFGNHGQAVGISNLWKNGGFFTGGVKGFFFALSIVVASYQGIELIG
                     MTAGEAENPKKTIIEAVQSTIGRILIFYIGAIFIIVSIYPWNQLSEIGSPFVQTFSKV
                     GITFAAGLINFVVITAALSGCNSGIFSASRMVYTLAINGKMSKKFLKLSRHGAPFYPV
                     VAISGGILIGLILNYLLPMFINQSENIFVFVYSSSILPGMVPWVVILISEIRFKKKHA
                     DKMHDHPFKMPLSPYTNYVTLAFLAIVLIFMFINPETRISLIIGLVFLLYMTIHYFVR
                     EKRTGK"
     gene            complement(83080..83976)
                     /locus_tag="U2K71_000290"
     CDS             complement(83080..83976)
                     /locus_tag="U2K71_000290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM7314409.1"
                     /translation="MYDENEWGENVRMTRRRQQKQAARKRMIMIGAAVAVFLIIAGGV
                     AGLYFHGKNSSEAKSTTITETTTKKTETKQTSESSTKTKETQTSEEKTANGLPEQLQA
                     IVNDYSKKIEEKTPALIEEYQTEIQGNQEGIAGLSAVANQKARELQAISDEGIRKLRA
                     AYQAAENKDGVDLDTLINQLSANYTNHVAKISDIYLQTSASLQAESTSTQDTTSSDSE
                     TAESVEASEMQARTTQDTTDSSETNTNSATSQAATTVVLEGEGPNQIAERTGVPVETI
                     LSLNGMSMENYFLTPGETLKLN"
     gene            complement(84090..84260)
                     /locus_tag="U2K71_000291"
     CDS             complement(84090..84260)
                     /locus_tag="U2K71_000291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314410.1"
                     /translation="MTNDWLIQQLDELSEKSEYQDRALLYEIKKLMAEIDERTMQLQG
                     EIDGQSWNHLNW"
     gene            complement(84257..84934)
                     /locus_tag="U2K71_000292"
     CDS             complement(84257..84934)
                     /locus_tag="U2K71_000292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM7314411.1"
                     /translation="MRTLIIISHPTIEESLNQQFFKEASAGLPATWHHLESSYPDGKI
                     DIETELQLLAQHDRIIFQFPFYWYSSPAHLKIWQDNVLERAAHVLKGKELGIVLTTGV
                     AEKEYQAGGKEEYTISEFLRPYQRIANKFHMTYLPPFVLAQFMYLSQEKRWEKLIAYQ
                     QYLSLEGKPSLTQRIDWFIQRVQENQKMQEEESEKQAYIVEALTEAKEQIEDLTFTLQ
                     EMKGTSL"
     gene            complement(85025..85930)
                     /locus_tag="U2K71_000293"
     CDS             complement(85025..85930)
                     /locus_tag="U2K71_000293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM7314412.1"
                     /translation="MEQSIFLKKEHQQVEKELYFDFCGFSKTMPFHSFGPAIRNDYIL
                     HVVMEGKGSYHVKNQQFQLKKGDLFLIRPGDSTFYLSDGEDPWMYCWLSFGGSAAEEI
                     IRQSLFREDQYTMVSSDIQKYIDIILECMKDTEETLENELQLTALTYRFLSVLLKDGG
                     KVSGGVQKVHSSLAVETVKYIEAHYAEKLSVEEIAQHLAVNRSHLSRVFKNHMGTSIK
                     EYLIGVRINRGAFLLSLTDESVESIAYQVGFNSLVVFSRMFKKSTGETATNYRKRMKN
                     ENFKNIALDTLKKQLEKQTAVSWTT"
     gene            86105..87559
                     /locus_tag="U2K71_000294"
     CDS             86105..87559
                     /locus_tag="U2K71_000294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7314413.1"
                     /translation="MKSKMIQRVSYALGAFGHDTFYVTLSTYFMVFVTSQMFAGTDKA
                     TNAKMIATVTSLVVGIRLVEIIFDPLIGGIIDNTRTKYGKFKPWLVIGGVVSSVLLVA
                     IFTNFFGLATHNTTLFIILFAIVFILLDSFYSFKDIAFWSMIPALSTETKEREKFATF
                     ARFGSSLGANGTTLVVVPIVTFFTYLVTHKHIEGASGWFWFGVIAAVISGGTAIITAL
                     GTKEVDTAIRQQNEKAGIKDVFKAVAQNDQLMWLALAYIAYAIANAATTAVLFYFFKY
                     VLGQPTEFWIVGLVAALLGLVAVPLFPMLTRVISRRYVYLGGIAMMISSYFFFTIAGS
                     SLPIVIIGLVLFYFPQQLIFLSALMTITDSVEYGQWKNGIRNEAVTLSIRPLLDKIAG
                     ALSNGIVGFVAIASGMTGNATAADITAHGVTTFKAYAFYAPAALMIFSAIIFAWKITL
                     TEKKHAQIVEKLENKLAPADTMEDELHDAVDPSH"
     gene            87575..88606
                     /locus_tag="U2K71_000295"
     CDS             87575..88606
                     /locus_tag="U2K71_000295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEM7314414.1"
                     /translation="MKITEKDFGQGYHLITLENKNKLALSISDLGARIVSLKSNDREL
                     VLGFDTAEEYIEKDPYIGASIGRTAGRIENGRFSLNGKTYQLATDPKTGHNLHGGAPG
                     FELKKWSYVILNGENEASVIFTTTSPDGEHGFPGTMDVEIRYTLTKDNIWRVTSRGTS
                     DQDTLFNPTNHVYFNLTGDASQSIDQHELWLNSEAYAPLRTDSIPIGVKENAAGSAFD
                     FQIPKKLASVFASDLDQKNLVDGIDHPFFLKETGLGKEAARLTSPDKRIQVDIATDAS
                     SVVIFTANFGTETPEMRNRKLAHHGGITFETQTAPGAERFSAFGSIHLKAGSVFETVT
                     EFKIKTRKE"
     gene            88613..89800
                     /locus_tag="U2K71_000296"
     CDS             88613..89800
                     /locus_tag="U2K71_000296"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HEM7314415.1"
                     /translation="MEIKRSLNTKFAETFGPKKTTQYFSPGRINLIGEHTDYNGGHVF
                     PASITYGTQGVAAPRDDNKILVYSTNFDDIGVISFTLDELEYDKKANWANYVKGMVLK
                     LKEAGHSIDHGFELLVEGTIPNGAGLSSSASLELLVGVVLEDLFDLKTDRLELVQTGK
                     KVENEFIGVNSGIMDQFAIGFGELDHAILLDTNTLKYEMVPVKLDGYAIVIMNTNKRR
                     ELADSKYNERRSECEEALARLQKTLPISSLGDLDEEEFESTIDQIGDDTLIRRARHAV
                     SENQRTLKAKAELEAGNLEAFGQLLNDSHHSLRYDYEVTGIELDTLVDAAQKQEGVLG
                     ARMTGAGFGGCAIALVKEENIPAFKNNVYDEYMKVVGYAPEFYVAHIGNGTTKIDPEA
                     VHV"
     gene            89956..91338
                     /locus_tag="U2K71_000297"
     CDS             89956..91338
                     /locus_tag="U2K71_000297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEM7314416.1"
                     /translation="MWANQNPVFVYEKEPSRDILCIDCKSFYASVECVEKDLDPLTTK
                     LVVMSYPSDSTETRGSGLILASSPAAKKAYGITNISRARDLPFPYPSDLYIVAPRMAY
                     YMEKNREINSIYKKYADEANHHVYSVDESFLDVTNSMALFGKTNAYDMAKLIHKDVFE
                     QTGIYTTIGIGDNPLLAKLALDNGSKDGKQMFAEWRYEDVPEKLWKISDITDFWGIGK
                     RTAKRLKQMGIHSVYDLAHADYYHLKSKLGVIGTQLYAHAWGIDRSFLGQEYTPKSKS
                     IGNSQILDRDYTRREEIEIVIKEMADQVGTRLRRSAAKTQVVSLWVGYSLGYLDPNGK
                     TGFHQQMKIEATNASRDLADALLTIFHQHYHYQDIRRVGVNCSDLVYATGLQLNLFED
                     PEKQINEAKVDFVVDTIRKKYGFQSIIHAHSLLQGGRAIARSSLVGGHAGGMSGLEGG
                     KDETDSKKIH"
     gene            91465..91695
                     /locus_tag="U2K71_000298"
     CDS             91465..91695
                     /locus_tag="U2K71_000298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314417.1"
                     /translation="MTLQEIDSILTEAFLSRKAVIIQLNLKDEFGRLLDEVEGHFIGE
                     AYEDYFMIDHQPILWEDVRYIELKETKKWFVT"
     gene            complement(91895..92563)
                     /locus_tag="U2K71_000299"
     CDS             complement(91895..92563)
                     /locus_tag="U2K71_000299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF421 domain-containing protein"
                     /protein_id="HEM7314418.1"
                     /translation="MIFSITMKLVFGLIGLLLVVRLLGKKSMSEITPFDLVYTLVLGG
                     ILEESTYDDNVHVGHVLFAIALWAVMIYGIERIVQKNEKVNRWVKGEPSVLIKDGVIN
                     MTELTNNHIEMEQLRAILRQQECFFLENAKHVILENAGQMSVLKKSDEDKALSILLVD
                     EGQIQHKVLQSNQLTEAWLMENLKKEGYADVKQLIYVEWSEEKGLYILNKEDVMHESY
                     RIDG"
BASE COUNT        26224 a        19567 c        16711 g        30286 t
ORIGIN      
        1 tatcaacgtt tcgagcaatg aaatagttat taacctacag acccttccat ctcataactg
       61 atcaatcgat tgagttcgac tgcatattcc attggaagtt ctttggtgaa tggttcaaca
      121 aagcccatga cgatcatttc agttgcttca gattcggtca ggcctcggct catcagatag
      181 tagagttgtt cttctgagat ttttgagact ttggcttcgt gttctaaaga gacttggctg
      241 ttgtggatct cattgaatgg gatcgtatcg gatttcgacc attcgtccat gatgatcgtg
      301 tcacattcga tatgagagat cgaacccgca ctgtttttgg caaaggttac ttgtcctcga
      361 tagtttactt ctccgccgtc ttttgaaatt gatttagaga cgatagagct agatgtattt
      421 ggtgcattgt ggatcatttt tgcacccgta tcttggattt ggtttgctcc tgcaaaggcg
      481 atggagagca tggtgcctcg tgcacctttt ccatctaagt aaacacttgg gtatttcatc
      541 gttgtttttg cacctaggtt tccgtcgatc cattcaacgg ttgcgccttc gtatgctttt
      601 gctcgtttgg ttactaagtt gtacacattg tctgaccagt tttggatcgt tgtgtaacgg
      661 caatacgcat cttttcttgt aaagatttct acgatggcgg catgcaggct gttacttgaa
      721 taagtaggtg ccgtacaacc ttcgacatag tggatgcttg ctccttcgtc aacaatgatc
      781 agcgtacgtt cgaattgccc cgtgttttcc gcattgatcc ggaagtacgt ttgtaatgga
      841 acatcgactt tgacgccttt tggcacgtaa ataaaggtcc cacctgacca tacggctgaa
      901 ttcagcgctg ctaatttgtt gtcggttggc ggtacaagtt ttgaaaagta ctctttgaat
      961 aattcagggt attctttcaa agcagagtca gtgtcagtaa agacgattcc taacttttcg
     1021 aattcatctt tcatattatg gtacacgact tctgattcat attgtgcgga agcaccggct
     1081 agataagctc gttcagcttc tggtatacca atcttttcaa atgtatcttt gattttttct
     1141 ggtacgtctt cccagtcacg agcgggtttg tcacttggtt tttggtaata tttgatggca
     1201 tcgaaatcga tgtctgaaag gtcggggccc catgtttgca ttggcatttt gttaaatgtt
     1261 tctagagatt tcagacggaa ttcaagcatc cattctggtt cgcatttgat tcttgacatc
     1321 tcacggatca cttcttctgt caagccttct cctgtactga aaaccggttc gacatcatcg
     1381 tgaaacccga atttatattc ttctaattct ggtacgccca tgttctcact cctcttcctt
     1441 gtggtaatga tggaaagctg cggtaccatc gttttccatt gcttttccta gtgctttcca
     1501 ggcaagtgtc gcacatttga tccgagcggg aaatttagct acaccaccaa gcatagcagc
     1561 atcgcctaat tggtcgggat ctttgacttc ttttccttgg acaagctgta gaaagtctac
     1621 agttaaatgt tctgcttctt ctattgattt tccgatgacg gcgtctgtca tcatcgaggc
     1681 acttgccgta ctgatggagc agccactgcc gtcaaaggcg atatttttga tgattccgtc
     1741 ttttacatcg acttgcaatt cgatcacatc tccacatgtg gggttgttca attcgatctt
     1801 gcttgtcgag tcagtcaagc ttccacgatg atgcggatgg gaagaatgat ccaagatcac
     1861 ttgacggtat aaattgtcta atttagatag tcccatgttg gaaaaactcc tttgttgcca
     1921 tgattgcttc aactaaacgg tcggcgtctt gcgttgtgtt gtagaaataa aagctcgcgc
     1981 gagcagtcgc tgatacttca aggtacttca atagcggttg cgcacaatga tgacctgctc
     2041 tgacggctac cccttccatg tctaaagctg tcgcgacatc atggggatgt aacccatcca
     2101 aattgaatgc aacgactcct gtccgaaaat tcgggtcttg tggaccataa acagtcaatc
     2161 catcaattgc ttgtaatttt ggcaacacat aagcggctag ttcttcttca tatcgatgga
     2221 tattgtccat accgagtttt tgcaaataat cgattgcatg gcctaatgcg atagcgccgg
     2281 cgatatttgg tgtccctgct tcgaatttcc aaggtagttc tttccatgta ctgtcgtata
     2341 attcgacaaa atcgatcatt tcgccaccaa attcaacagg ttccatttct tctaaccatt
     2401 ttcgtttacc atataaaaca ccaatacctg ttggtccgca cattttgtgt ccgctgaaag
     2461 caaagaaatc acagtctagt gcttggacat caatggccat gtgcggggca gattgagcac
     2521 catctactac gatgaccccg ccgttttcat ggacaagttc agctaattct tgaacaggat
     2581 tgaagacacc taagacattg gatacatgag caatggagac gattttggtt ttgtccgtaa
     2641 tctttttcgc tgcatcctcc atatccagga agccttcttc tgttagttcg atatatttca
     2701 agacagcacc tgttcgtttt gccaactgct gccacgggat aacattagaa tgatgttcca
     2761 tgtaagagag cactatctcg tctcctgctt tgatatagcg gtcgccgaag gttctggcta
     2821 cccaattcaa gcttgtcgtc gtaccacgag taaacagcac ttctgctgtt tcttttgcat
     2881 ggatgaacgc ccgtactttt tctcttgctt gttcataatc atgggtcgcg cgttctgcta
     2941 acgtatggac accgcgatga acattggcgt tatcgtgttc gtaatagtac gctaatgcct
     3001 ggagtacttg ttttggcttt tgggtcgtcg ctgcattatc cagataaact aaaggttcat
     3061 cattgacgtc ttgaaataag ataggaaaat cattgcgtag ctttttagca tcgatcatcc
     3121 attcaacttc ccttcgatta cttcgactaa ttctttccga acttgttcta cagggattgc
     3181 tgttaagaca gaaccgagga atcctcgaat cactaagcgc tctgcttctt ctttatgcaa
     3241 tcctctactc atcagatagt acatttcttc tggatcaact cgtcctacac tggcagcatg
     3301 tccagcagtt acttcgtttt catcgatcaa aagaatagga ttcgcatcac cacgtgcttt
     3361 atcagaaagc atcaagacac gactttcttg ttgggcatct gcaccttttg cccctttcaa
     3421 gatgtggccg ataccattaa aggtcaatgt tcctttttct cggatcactc cgtgttgcaa
     3481 aatatgcccg atcgtatgag gggctttatt ggttactcgt gtatcgattc cttggatttg
     3541 tctgcccgag ctgattgcca cgattttgac ttctgcgtgt gcaccttctc ctgctaaatc
     3601 tgagtcaaag tcagcgatca catgaccgtc attcatgaca ccaattgccc agtcgacaga
     3661 agcatctcgt aaaatgtgcc cacggcgatt catataagaa gtaatatttt ctcctaattg
     3721 atctacggct gaatatttga tttttgaacc agctttagcg atgacttcca cgatgatatt
     3781 acctgatgat tttgctactt gttctttggt tgtctggaaa cgttccagat aagaaaattc
     3841 actatgttca tctgccacga tcaacacatg cttgaagaag tgctcatcgg atgcgccatc
     3901 ttgaatgaat aaggactcga gtggttcatc gatcacgaca tttttaggca catacaggaa
     3961 aataccgctg ttcataaaag ctacatggag tgcagtcaat tggtcttcgt tcatgtttac
     4021 tgcttttttc atataatatt cttggactag ttctggatat tcgatcattg cagtgaacaa
     4081 gtcggtaaaa atgactcctt tttctgctaa ttccacaggc agttgttcaa aaatcgtcca
     4141 tgaaccttgt tggacaagca caggattgtc tttcattgca tcaaatgccg gaatcgtgcc
     4201 tgtttcactg atggtttgtg tttctttcac atcaaataac gaccagcgat ggaatttcac
     4261 ccgatcgata tgcggcaatg gcagttcatc tgctttcgct agtgcgtctt gacggaaggt
     4321 tctcatccaa tcaggttcgc ctttacttaa agaaaaatca tttaccgcgt caagataatc
     4381 gagccagttt ttttctttca ttcttgctcc cccttacgct tcttcttctt tatagtcgat
     4441 tcctaattct ttactgatac cagcgtagcc ttctgcttcc agacgtttag ctaggtcagc
     4501 attccctgtc atgactactc ggccttccat catgatgtga acgacatcag gtgtgatata
     4561 gttcaataat cgttgatagt gggtgatgat caatgcaccg aaattgtctc cgcgcatttc
     4621 atttactcct ttagcaacga ctttcaaagc atcgatatcc agacctgaat cgatttcgtc
     4681 taaaattgca aatgtaggtt ctaacatcaa caattgcagg atctcattcc gtttcttttc
     4741 tccgccagaa aaaccttcgt tcagataacg ttcagccatt tcttctggca tatttagtaa
     4801 agccattttt tcatctaact ttttcaaaaa tgccataaca gaaattttgt cgttttcatc
     4861 acgtttcgca ttgatcgctg cacgcatgaa ttccgcattc gtgatacctg gtatctcact
     4921 tggatattgc atagctaaaa ataagccaag tcgcgcacgt tcatctactt ccaagtctaa
     4981 aacatttttc ccgtcaaaca aaatttcgcc ttctgttact tcatagtttg gattgcccat
     5041 gatagcggca gataaggtag attttcctgt gccgttaggt cccatgatgg catggatttc
     5101 tccggttttc attgttaggt ttacgccttt aaggatttct ttttcctcga tcgacacatg
     5161 tagatttttt atttctaaga cagacatgca ttataccccc aaattctctt tcgttctcac
     5221 tcattttaga ctaattgaga atgaagcgca atgaccttcc ttactaaaat caaatatctt
     5281 ttatttatta ttaaacaatt ttgatttcta aattagaata ggtataaaaa agatgagaaa
     5341 taagccatta cagacttatt tctcatcttt tttgttccat accttatttt gcatcagcct
     5401 ttactggttt aacagagccg ccccatttgt ctgtgatcca ttcttggact tctttgcggt
     5461 gaagcacttc aatcagtttt ttgattttgt cactgtcttc atcaccttta cgagtcgcaa
     5521 taatattcac gtaaggagag ttgtcctttt caagcaatac ggcatcttct aatggattca
     5581 accctgcgcc gtaagcgaag tttgcgttga ttgcaactag gtcaccttca tcattttggt
     5641 acgtagaagc taaaagtgat gggtcgatcg tatggttgaa cttcaagttc ttagggtttt
     5701 cttcaatatc atcgaatgtt gctgtttcca gatctactcc atctttgact ttgactaagc
     5761 cagcatcttg tagaattgtg atgatgcgtc cccagtctga ttcagaattt gaagtaatga
     5821 ttgttgcacc atcttttaat tcggaaacat ctttgacttt tttagagtac aagcccattg
     5881 gttcgatgtg gacagcaccg gcattgacaa agtcatatcc tttttctttg atttctttgt
     5941 tcaaataagg aatatgctgg aagtaattgg catcaatgtc gccttcttcc aaagattggt
     6001 tcggtaatac atagtcatcg aatttgacga tttcaagatc gattccttca tctttcaata
     6061 atggtttcac atgttccaaa atctctgcgt gcggtgtcgg tgaagcgccg actttcaatg
     6121 tcgtgtcgtc tgctttattt gctgaatctt tgctgtcgct gttgttaccg catgctgcta
     6181 atcctacagt cagcaaaagc gctgctgcaa atccaaaaat tttctttttc atatcctaca
     6241 tccccctata tcctattatc ttttgtctgt tttcttagtt aagaaatcac cgattccttg
     6301 aacgataaat acgattagta aaataattaa agtcgcaacg aatgtcactg ttagattacc
     6361 acgctggtag ccttcctgcc aagcaagacc tccaagtcca cctgcgccga tcgcgccggc
     6421 catcgcagta aatccgatca tcgagatcgt tgtaactgtt aatccagaaa tcaatgcggg
     6481 aatactttca ggtatcaaga ccttccagat gatttcccag taacttgctc ccattgcatc
     6541 tgctgcttcc agtacaccag ggctgacttc acgaaaagca atctctacta gacgggcata
     6601 aaatggtgct gcggataaaa caagagctgg aatggctgct tgcgcaccta gcatagtgcc
     6661 tacgattgct ttagtaaatg gaatgatcaa aaccatcagg atcataaatg gcgttgaccg
     6721 gaaaatatta ctgatgatcg atacgattcc ataaatgatt cgagcatacg gtttgttttt
     6781 gcgtccaata gagtaaagga tcaatcccaa aactaatccg atgatcgtca ctaagaccat
     6841 tgagattaat gtcatatata gtgtatcaag gatagcctga ttcatcgttt ccgggttgat
     6901 ttgtttaaaa tctagatatg tttctgaaaa gcttttatcc acgatggatc acctcaattc
     6961 ctactccttg ttctttgatg aacacttctg cttgatctaa ttctttggtt tcaccagtca
     7021 tcaagacagt caatgaacca aaggaacctt ctttcacttg tttgatcttt ccatacacaa
     7081 cgttgacatc gatgttatag cgtcgaatcg cttgggaaat gactggctcg ttggcatttc
     7141 tttcgctaaa ggtcaaagtt gccaaacggc cgcttggatt ttctttgatg agttcagcta
     7201 atagttctgt cgaatcagct tctggttcga tgtcttgttg tacaaagcgt ttggttactt
     7261 cttttttcgg ttgtctgaag acagtcaata cgtctccttc ttctacgact ttccccaact
     7321 ccatcacagc gactttgttg cagattttgc ggatcacatt catttcatgt gtgatcaaga
     7381 cgatggtcaa attcaatttc ttattgatcg ctaacaataa gtctaatact tcttctgttg
     7441 tttgcgggtc caatgcgcta gtagcctcat cacaaagcag gatctcagga tcatttgcca
     7501 atgctcgtgc gataccgacg cgttgtttct gtccgccgga aagttgtgaa ggatatgctt
     7561 gtccgcgtcc ttctaacccg actaaacgca acagttcttc tgcccgttct ttacgtttgc
     7621 tctttggtac gccagctaat tccagcggca gctggatatt ttcaagtaca gttcttgacc
     7681 ataacaggtt gaaatgctgg aagatcatac cgattttctt tcggaatgta cgtaattctt
     7741 tgttttttaa ttttgtaata tcttgatcgt ttacgatcac ttcgccgctc gtgggaagct
     7801 caagtccatt caggagacgg actaatgtac tttttcctgc gccagaatag ccgacgattc
     7861 cataaatatc ccctttttca acgtttagtg aaacgtcatt gacggcttgg atgtttccgt
     7921 ttttcccatt gaatgtcttt ttgacattcg ttagttggat taatgacatg ccattcacat
     7981 ctttctctta taatttttca aacaaaaaag ccttcgccct cttgccttaa gcaaaaggac
     8041 gaaagcttga acttccgtgt taccaccttg atttaccgtt acttcgcaat aacagcctct
     8101 tccagtactt ggacttagct tctttccgta tactgtcgcg ctgtaacggg cactcccgta
     8161 gtaagtttgc ttacgctcgc ttactctgct ctaagaccat cttccatctc attttcgtta
     8221 cccattttca gcaaacaggg ctctcttcaa acgtccgtga catgtactct tcttttcaaa
     8281 gctttctcgt ttcatatagg attgcttatg attatagcaa ctggaagaat cagtgtcaac
     8341 ttttttcaag tgattttctg attttataat tcgtcaaatt cttttggatc aacatctttg
     8401 aaacttaaga tccacgcatt catttcatct gaatcattca agatattgat atcttcaccg
     8461 attttatcat taacagaaga aactgtaccc gttagcgggc tggcaaattc attgaccgct
     8521 ttttccgctt caacttcgat cagaggttca tcttttttta gcgattgtcc tgcttttggc
     8581 agattggcaa acgtgatgtc tccaagttct tcttgcgctt catttgtcaa accaacacag
     8641 tattcttttc cattaaataa gatccaaagg ttgtcctttt tcttcaaatg cttgcctgcc
     8701 attttatatc agttctcctt cataaatttt ttcattgaat ccaattgcaa caagctggtc
     8761 ctctcgtaag atcagtggtc gcttgatcag cataccatct gtcgataaga cttggctcgc
     8821 ttttgaagcc gtaaactcgc ctacttgatc tttcagtcca agttcccgat atttcatccc
     8881 gcttgtgttg aacaggcgac ggattggtac ttgactcgtt tctagccaag tctcaatcgt
     8941 tgaggccttc ggtacttctt tgatcatatc aacggtttga taactaacgc catgctggtc
     9001 taaccacgct ttggcttttt tgcatgttga acatttcggg taccagtaaa aggtgtacaa
     9061 ttttcttacc tccttgaaga tcttgcaggt gcttcatttt ggtatctcat cgataatatc
     9121 aacccgatac cgatcatgtt ccctaaaatc gaggatcctc cctgactaat gaaaggcaat
     9181 gggatccctg ttaaaggaag taatccgata tttgctccaa tattctcaaa gacatggaac
     9241 agcagcatca tgatcaaacc gcttgcgata taggcgtaaa attcattatt cgtatcgaaa
     9301 cacacccgaa tcatacgata gattaaaata aaatacaaga aaatgacgaa tgcaccaccg
     9361 ataaagccga agttttcacc gataacggta aaaatcatat cggactcacg aacaggcaca
     9421 tagatattgc ttacgttgaa tcctttacca aacaagccac cggtaccaat tgccaaaagt
     9481 ccttgggccg gctgataact cattcctgac gtatcatgga aagggtccag ccatgaatca
     9541 atacgtgcaa actgataaga tttgaatcca actttataaa gcaagtctct tccgccttca
     9601 gtcgtaacca agaagatcgt tcctgctcca acgactactg ccaagattac tacaggtgcg
     9661 atgatgcgcc aagagattcc agacatcagg aaaatcccgc caaagatcgc aagaaagaca
     9721 agcatcgttc caaagtcatc ttgggccatg atcaatgcta aaaccggtat tgtcacgatc
     9781 aagagcttcc caatcagcca aaagtccgat ttcatcgtcc gttcttttac ttgcgtatta
     9841 tgtttagtta cgatcagtgc catcatcaag atgtacgcaa tcttcatcaa ctctgctggc
     9901 tggaaagtaa acgcaccgaa tcgaaaccag tttttagacc ctgttgaagc agctaaattc
     9961 tgatcataga atttgagcaa gagtcccatt accactaacc ccaaaccata aaggtaagga
    10021 gtcagtttcc agatccattt ggaattcaaa tgcatgatga caacgattgc tgccgctcca
    10081 actaaatacc agacaacttg catccctacg cctttcaaga ctgctggatt gttcggatca
    10141 tggctaagtg ctacatataa tgagagcaga ccaatcaaac ataacagaaa gactggtaaa
    10201 atcacgccat aatcaatacg attatcgttc gaagctttta attttttttc catgtttcat
    10261 cccttttctt aaaaaccata ctgctgatta ttatagcggt ccagttagat aatggaaagt
    10321 ctttccatag acatttggga aaaattaact ttccgtttgt gcagttgctt gagcaaatgc
    10381 agtacaaaag cctactgctt atctttagtg tatcatagaa ctttttttga tacatgcaat
    10441 tatctaccag ctgagattct cctcgctgtg agaagtttct gtgctttcat tgtatcgaag
    10501 gatctcttcg gcaatctcta gcagctgatc agaaaccaga ttggttcgtt ggatgaaata
    10561 aaaatatcga tggaaattca acggagtcat tggcacattt tctggaacag agcgcttaga
    10621 caaaatcgag cgaccgattc cttccttcag caacgcaaca attacatcgt tacttttcac
    10681 gatcatcttc ttatcttgga tattcatctc ttcaaaataa cgctgcgtgt aatgaaaaac
    10741 accagagttt gcttctctta caagccacaa tttgcttttt gggtccccag cgatgactag
    10801 ctggtcttcc atcaaagagt atctttgtac gccggaagtc tctaatggct tttcgataaa
    10861 accaaaatcg gattcatgtt tcgctacttg ttctaatgat tcatgagaat tcgtgatttc
    10921 gatctcaaaa tgcactttag gaaacctttt gatcagatgt ttcatcaact gaggcaaaag
    10981 atagacagca aaggtgtggg aagcaacgat ccgacatact tcttcttcat tttgttcatt
    11041 gagcaatttt tgctgagttt cttcccattc ttctaacaaa accagtgtcc gttcatataa
    11101 taagtcggct tgcttagttg gaagcatctc ttttcttccg ttacgttgaa aaagatcgat
    11161 attcaattcc cgctccaact gtttgatttg attcgagact gctggttgag ataaaaacag
    11221 tacttctgct gctctggaaa aatttcttgt ttcatatact gctcgaaaag tcgttaataa
    11281 cttaaacatg gcaccacttc cataactttt tatgataatt actataaaaa ctatttgatt
    11341 taaatataca aaaaaccatc tataatagaa atgtacaaca aaacagttgc tgatccaact
    11401 agtaacttag atgaagacaa tcagctgtct gcttgctcgt aatcatacat tcctattcat
    11461 tttacaatgt attcgttcat cttggtacta aaatctatta aataaaaagg agaatattat
    11521 atatgaagaa ctttttgaaa attattccga tcccgatctg cgggttgatc cttgggttag
    11581 cttcccttgg aaatctgttt aaagactatc accatgttgc tttaggcaat ctaactggcg
    11641 gtatctctat gattcttatg atattcattt ttgttaaatt gtttatctta ttcgaacaca
    11701 cgaaacaaac gttgcatgat ccaatcatcg cctccgtttc accaactttt acgatgtcac
    11761 tcatggttat ttgtacttat tttgtttcat atgaaagtat tgcaccaatt gtcaaattca
    11821 tttggctgtg cgctgttatc ttccaagtga tccttgtctt ttatttcaat tatcaccatg
    11881 ttatcaaagc tgatctttca attgaagcaa tttatccaag ctggtttatc atttacgttg
    11941 gatttggggt catcactgta actgccggga acttcttccc acttattgga aaaattttct
    12001 tttggatttc attgatctgc tatgcgttct tattgccgat catcatcaaa cgtgtctttt
    12061 tcgtaagaaa tatggctcat ccgacacgtc cgctgattac gatcgttgca gcacctggtt
    12121 ctctttgcct aacaggttat cttaaaaact ttgctgaacc taatttttat cttgtcgtag
    12181 cattgttcgc tttatctcaa gtattgtatt tcgttgtgct gtctatgctt ccacgtttgt
    12241 tcaagcttcg tttttatcca agctatgcag cctttacatt cccgcttgtt atctctgcaa
    12301 cagctatatt tactactgtt cattacttca acagtttagg tatctatagt agcgtattgg
    12361 atttcttaaa agttgcggaa tgtctgtttg caagtgtagt tgtcttctac gttcttgctc
    12421 attacgtacg tttcttaatt aaagaacata gcaaacatgg aaaatttgtt tctaaaaaaa
    12481 tcgcttaata atcgattcaa taggttgtga aaaagctgtt ctgcatcaag taataagaac
    12541 ggccaaacaa aaacagctgt tcatgttttt gtactggttg ggcttattac cgcagatgca
    12601 agcttttgaa caccgtttac caagaggtcg taaaagaagt ggtcagcttc aagaaataag
    12661 aaggaattta cgaaaattgc ttctcaaatt tttgtgaaat ttcggcttat ttccgtagaa
    12721 gctacttctg gaacaccgtt tattcgaaac aagaggtcgt acaagacttt tgtcacgacc
    12781 tcttgttctt ttgtcaaaat actggtttag tgctactatg ttcgagtatg cttttagtat
    12841 ttattgacga aaggaaccga tcaaatgaaa tcattgtcac atccaataaa atctgtaaag
    12901 gattcagcgg tccgcgaaat cttacccggt ttactgcttt cttttgccat cgctgccttc
    12961 agcaggcttc ttgctattta tctgccacag ttaggtggag cgactattgc tattttattg
    13021 ggtattttat taggaaatac tttttttaaa cagcctgttc tagcaatcgg cacaaaattt
    13081 gctgaaagca aactattaga attctctgtt tttttactag gtgcaactgt cacagctcaa
    13141 acgattgctc aaattggtgt aaaaggaatt gtttttgtgt tactgcaaat gtcagtcact
    13201 atcatcggtg cttacttgat tggtaaaaaa ctgcaattca gcgatacttc ttctttgatg
    13261 atggctggag gaaacgctgt ttgtggttct tctgctattg cttcaatcgc cccttccatc
    13321 catgcgaaag aggaagaaaa aggacagatc atcacactag tcaatctact gggtactgtg
    13381 atgatgttga ctctcccttt tcttggatta cagttgtttg gtggaaatct cattgaaaaa
    13441 agtgcacttt tgggtggtac actccaatct gttggtcaag tagtcgctgg ggcaagctta
    13501 cttgacaatt ctgttgtcga attcgccatg ctatttaaga ttctacgtat tatgatgctc
    13561 gttgttgttg tctatctgtt tgaaaaattc atgcacaaga aacaaccagc tctgtttgaa
    13621 acgccaactg atacgcagaa aaaacgaaaa ttaccgcttc cttggtatgt attagggttt
    13681 gtgatctttt gtttggtcaa cagcttgttt tctttacctg tagttattga ccaatcggca
    13741 cattttatca gcagctggtt tgagatcact gctttagcgg caattggtct gcgtttagac
    13801 tttgcaaaat tcatgaaaga aggtacacgc ttcttatctt atggattagc tgtcgggctt
    13861 ctccaaacag tgatggcttt actatttatt tggctgatca aagtttagaa tgaacatgaa
    13921 aaaacagttg tgattctcgc atggaacaat gtccgaatca caactgtttt taattttgca
    13981 gatatccttc taacaacaac aattggcgtt tgatttcaac cccatctatt gcgtttccca
    14041 tatatcctgt cagttttcca ttttgcccga tcactcgatg acaaggaata acgattggca
    14101 atggattggt tcgatttgcc tgcccaatcg ctcgcacagc tttcggacta tcaacagcaa
    14161 cggctacttc tttatacgtt cggatttctc cataaggaat ggtcgacaaa gctttccaga
    14221 cattttgctg aaaaggcgta cctctcttta tagataaagg aacatcaaat tcttttcttt
    14281 ttcccgcaaa atattcttca aactgttctt gtgtgcgcgt taaaattcgc tgattcgctg
    14341 attcgctttc tgaaattggc tgaaaagata cagcagtcaa tccggattca tctgcatcta
    14401 accataaaaa gccgataggt gtgtgtattt tcattttgtt tgctcctagt caatagactg
    14461 gaacttcctc ctttttctct atccaaaact acttgccaac taataaaaat tgttcttaaa
    14521 agtttgataa accgaggaaa catggtaaac taaatacgtt agtttacaaa aagaatacct
    14581 gttcttttac gtaaaatcaa attttaaagg tgttatttaa ggtttattaa attttgaatg
    14641 taaaccatat ataaacatac gattgaaagg atagaaacga tgaaagtatc aattgttgtc
    14701 ccatgtttta atgaagaaaa aactgttcct cttttttttg atgaggttga aaaattcaaa
    14761 ggtgcccatg agttcgaata tttgtttatt gacgacggat caaaagacgg tacgttagca
    14821 gcaatcaaga agctagcctc agcttatcct tatgtccgct ttgtctcatt ttccagaaat
    14881 tttgggaaag aagcggctct gtacgctgga ttgcaagctt ctacaggaga tttagtgacc
    14941 gtgatggatg ttgatctgca agatccgccc gaactgcttc ctgagatgat caaccggatc
    15001 actacttctg atatcgattg tgtcggcacc cggcgttcga ctcgtgaagg cgagcctgcg
    15061 atacgcagtt tctttgcgaa aaaattctac cagttgatca ataaaatcag cgatacagaa
    15121 atggtcgatg gcgctcgtga tttccgcttg atgactcgtc aaatggtgga tgccgtccta
    15181 gaattaacag aatacaaccg attttcaaaa ggattattta gctgggtcgg atttaaaaca
    15241 gaatatattt cttatgaaaa ccgcgaacgt gtggctggcg aaacatcttg gtccttctgg
    15301 aaattattca actactcgat tgatggtatc gtgaacttct cggatgcacc gttaacgatt
    15361 gcttcattta tcggcgcttt gtcttgtgtc ggttctggta ttgcaatttt gttcattatt
    15421 ttgcgggcat tgttgtttgg tgatcctaca tcaggatggc cttccatggt ctcgatcttc
    15481 ctgttcattg gtggaatcca attgttatgt cttggaatca tcgggaaata tattgggaaa
    15541 atcttcttag aaacgaaaca gcgtcctgtt tatatcataa gagaaacaga aaaagtgaag
    15601 ccaaatgaat tgaagcctca ccaataatat acgtcaaatg aagtaaacat ttttttaaat
    15661 gaaaaaggac tgtgacaaaa gtcttgtcac agtccttttt tccgaataaa tggtgttcca
    15721 gaagtagctt cttcggaaat aagccgaaat ttcacaaaaa tttgagaagc aattttcgta
    15781 aattccttct tatttcttga agctgaccac ttctatcaca acctctttca tatcttatta
    15841 cttcttcatt gctgctttta gtgctgctgc taatgggctt tcttctggtt cttctgcttg
    15901 tgcatatttt ttcagcaagc gtcgttcttc atgtttggtc attttcttct tctgctcttt
    15961 tttatcaagc aatttttctg tcgttccatc gtatttacaa cggaaataag caccgttttt
    16021 accttcgatg atttccattt tccggtgaca ttgcgggcat cgatgattag agacttttgg
    16081 atcttttctt ctgcggtagt tgcaatctga attggtgcag acatagattt tgccgtcacg
    16141 tgtgtttttc tcgcgcagat tggccccaca ttctgggcat ttcttttggg taatcgagaa
    16201 atcttgatat tcttgtttgc ttgatttgat ctcattcacc aagcgttttg tttctttttc
    16261 gattccattc aagaaggttc ctgcttgttt ctttcctgca gcaatagctt caaggtcttt
    16321 ttcccagctt tctgtcaaat ctggtgttac taaagaggga tttaccaata ctaataattg
    16381 cttaccttta ggagatactt gcagtccttg aggggtacgt tccatcagtt cagatttgat
    16441 tagtttctcg ataatctctg cacgcgtagc tggggttccc agactgtatt tttccatttg
    16501 accaagcaag cttgcttctg acaatggttt aggcggtgca gatagttctt ttttgatggc
    16561 gaaatcggga ttgattctca ttcctttttc ccatttgact gtttccgtct cagtttcagt
    16621 ttcgcctttc catcctgctt ggacgacacg attttgccga aaaatgaatc gttctttacc
    16681 aaattcaacc gtaacttttg tttggctgac tttatggggt tgagcaaaca gtcctaaaaa
    16741 acgagagaca atcatttgat agatttttcg ctcttcatta cttaattttt cataacgtgc
    16801 tctgttttca gttggcaaaa gtgcatggtg atcggtcact ttactgtctt ggaatacttt
    16861 tgtctgttgg acgatggctc cgtttttcaa atactgcttg acctcaggag caaagtcgct
    16921 gactgcttgc aatcgttctt tcatcgtact tttcatgtca tttgtcagat atttgctgtc
    16981 tgtacgcgga taagtaacga tcttatgggt ctcatacaaa ctttgtacaa gacttaatgt
    17041 tttctttgca gaatagccat agcgttgatt ggcttctctt tggatctctg ttaaatcata
    17101 aggcaatggc gcagattccg tttttaattt ttcttggact tctgtgacag ttcctttgct
    17161 tgtactgatc aattgaacaa gtttttgtgc ttcttcttgt gtttttaaag caaattgatt
    17221 tttttgaagc attttggctt gttcttttcc agcttgcaac gtgatcgtga aatacgtttg
    17281 tggacggaac tgctcgattt tcttttcttg gtctctcact aatgctagtg tcggtgtctg
    17341 cacacggcct gctgataaat tatccgcgta cttcacagtt aaggctctgg tcacattcaa
    17401 tcctaccagc cagtccgctt ttgcccgggc aagagcagat tcatacaatg catcatattc
    17461 tttcgcaggt cttagttgct tgaatccagt tttgattgct ttatctgttt gagaagagat
    17521 ccataaccgc ttcactggtt tattgaaatg cacatactct aagatccagc gagcaacaag
    17581 ttccccttct cttccggcat ctgtcgcaat gactgcttct gagacatctt ttcgctgagc
    17641 cagctgcttg attgctttta attgatgtcc tgttttagga agtggtttga ttcctagttt
    17701 tttagggatc attggcaacg tgtccatttg ccagctctgc cattccttat tcagatcttc
    17761 cggcattttc aatcctaata aatgaccaag ggcccaagta acgatcacct tgggtccttc
    17821 ataataattt ttgtgctttt gattagcacc aagcaccttg cttaggtctt tcgcaacgct
    17881 tggtttttcc gctagaatta gttgtttcat aaaattcctt tctgctttct actgtgatgg
    17941 gctgatgatt tttcaacaac gcaagtccct tgtattgctg taaatcatcg tcacactctt
    18001 cacaccagcg ctcatctccg tcattccaaa aggcgctgag aaagttactt tttgcattga
    18061 tatattcata attttgatgg atttcgttcc attgtttttc gtacgcttgt cgtgcttctt
    18121 cgaaagactc gaatgtttct tcttcttcaa tatcttcttc ccagtcttca aaaaaccacc
    18181 aaggctcatt gtccccgaac atggtaatta cttgatacat actcgtgctc cttctttcag
    18241 cttttctaga ctcatcttag tcacttttca agaaagttgc aagaattttc tcttataaaa
    18301 gggtcattta atgaacatct taaaattgtt acaaatgaat ctgattgttt aaggcaaagt
    18361 agcgataaag ttattgatca tccaaaaata gctgtcttct ggatcgattg ttggttcttt
    18421 tgtaaagtat cctaaaaaac gtaaggaaag ataaccgtgg atcatactac gaagttccct
    18481 gcttcgatga actttttctt ctttcgttaa ttgaaaagcc tcaaatatct gcaaaatgat
    18541 ctgattcgtt tcatggattc catctaataa aacagcttca ttggtatgcg gtacactgat
    18601 cagtaactca tacactgctt gatttgaaaa agcaaaggat cgataagttt ctgcatagat
    18661 ctgcagtgct tcctgaccac tttttcccac aagagcccgt cgcagctggt cattaagttc
    18721 ctgcagaagt ttcgctgtta gcgctgtttt gacatcacga atatttttga aatggttata
    18781 cagcgaggga gccttgatat ccaagcgttt ggctaaatgc tgaaaggtta tttgctgaac
    18841 atccatctct tctgctagtt ctcgaaagca gtcgatgatt ttctcttgag acagatttct
    18901 ttttttcatt tccatgtttc ctctcataaa ataaatcctt cactctttca gtttaccacg
    18961 aaaaagaaag agaaaaaaga gactttcatt gataaactac atattgtagt ttataataaa
    19021 gagctgtaga tgaaaatttt cagaaagaag gagtctaatt ggctgcaaga ataaaggaaa
    19081 aattatttag gaaaagagaa acttgggaac aaaacttgtt tgtcttatgg tttggaacat
    19141 ttatggctgg tatcggattt agtttagtca tgccttttat gtcactttac atagatacgt
    19201 taggtaatta tagtacgtca caattgaact tttggagcgg attgactttt tcatctactt
    19261 tcttggtgac tacgcttatc tctccttggt ggggaaaatt agccgaccaa aaaggaagaa
    19321 aattgatgct gttgcgtgct tcattaggaa tggcaatcgt catttctttg atgggttgcg
    19381 tgactagtgt gtatcaatta gtcgcattgc gtctgcttca agggattttt tctggttata
    19441 tcagtaatgc cactgccctt gtcgcaactg ggacgccaaa agaaaaaagt ggtcaagttc
    19501 ttggaacctt agcaactggt tctgttacag gaacattact tggaccgctt cttggcggga
    19561 tctctgcttc agcgtttggc tatcgcccta cctttttcat tacagggatc atcttatttt
    19621 tcgtcttctt gttgagtctg tttttcgtcc atgaaaaatt tgtcccagtg aaaaaagaaa
    19681 acatggtttc tgccaaacag attttcaaag acttgaaata tccacacgtc gtcatcggta
    19741 tgttcgtcac aacgatgatc atccaagcat ccaacaattc cattagtcca atcatcagtc
    19801 tgtacattcg ccaattgctg catggacatg ggaatgttac gttgatcagc ggaatcattg
    19861 cttcgatacc tgggattgcc actttgatag ccgctccaag atttggtcgc ttaggtgaca
    19921 agattgggag tgaaagaata cttgcgattg gtttgggttt tgcgattctg gtttatatcc
    19981 caatggcatt cgtcacgaat gtttggcaat tagctgcctt acgttttttg attggtatct
    20041 ctgatgcttg tctgctccct gctgtccaag cattgatcac aaaatattcg cctcatcaag
    20101 ctgctggacg gatcttcagt tataaccaat ctttccaagc aactggtaac gtagtagggc
    20161 ctatgatcgg ctccagtgtt tccagtgcat ttggttatcg cggcgtcttt ctttcgactt
    20221 ctcttcttgt tttggtcaac ttcttgcttg tacgacacaa tacacaagag atccatcagc
    20281 aagaaactgc agaagaaaaa gcttcaggag tctccatagc ttcagggcac tctccttctc
    20341 atagacaata agcgtaaatg aaaaacactg cttgaacagt tgtcacagac tgtccttgca
    20401 gtgtttttgt ttaggcctgt tttttcaaga actcagataa gaggaataat cccttttcca
    20461 aggtttcttt tttacaacag taacctagtc gggcatgctt cggtaaatca aaggcacttc
    20521 ctggtactaa tagtactcct gtttcttcta ataaattgat acaaaatgtc tggtcatctt
    20581 cttcgatggc taatttaatg aaagaagtag agacgtaatt gggagcgaca agttcaactt
    20641 tcggttcatt ggctacccat tgttttaaaa tcacaaggtt ctctgtaatg atctttttgt
    20701 ttcgcgccaa tattttttct ttattcttca atgcatgtac agcaagatca tctgacagga
    20761 ctccgccgca aatcatcgtg tagtctcgat actttcgaaa aacttctgcc aactcaggtc
    20821 cagtagctgt ccagccaata cgaatacctg gaatggagta ggttttggat agggaattgg
    20881 ttgctatacc tttttcatag acatctacga tcgacaagaa ttcaccttta tccgttaaag
    20941 gcgcatatac ttcatctatc aatacatatg catctactgt acgagcaatt tctgcgattt
    21001 tttctaaagt tttccgatct agcaatgtgc ctgtaggatt gtttgcacta ttcaaacaga
    21061 tgatttttgt ttcaggtttg actaaggctt ctagctgttc taaatcaaac tgccaatctt
    21121 cttcttcctt caaatgaaca aagtcaacag cggcacctaa agatttgggg atgtcatata
    21181 attgctgata agtaggaagc atcgaaacaa catggtctcc tgcttccact aacgcgtata
    21241 gagctaaaag attagcacca gtcgctccat tcgtttgtaa gatgttttcc ggatcgacgg
    21301 tttgataaag agaagcgact tcttgtttga aaacatctga tccttcgatc cagccatagt
    21361 ccaatggttc gtttttgcgt ttttcgaaaa atttacttac gctagtccca tctaagccaa
    21421 cgagttcgtc taatgtcaat gcttcgatcg agctttgtgc aatatcatat accgctttct
    21481 tttcccattt atttagccat tcttctacac cgaaatttgc gattttcatg tttctttccc
    21541 ccttatatct tgatccagcc tacagcaagc aacccgattg aaagcagtgc ggccacacaa
    21601 atcagcccaa tcattacttt ttcttttccc gtaatttgat gtttggtttc acgacaagcg
    21661 atccagtaga agcaaaaacc aggaagatag gcaatactac ataaaaggac ataagaaaat
    21721 cctgctaaaa tgatgcccat caattcaaaa actacagcaa tcccaccgat cactacttgt
    21781 tttttgattc cttcttttaa agaaaactgg acttgataga tcccgacaag tagataacaa
    21841 atcaaaattg ccgctgtaca taaagaatag gcaaattgat aggcataatc agtaattaag
    21901 aaagtcaata agaatagatt agtcaagatt gcagtaacaa ctaacgaata agttggggca
    21961 ttttttttat tcgtttttcc ccaacctttc ggcaacagac cgtctcgagc catcaacatc
    22021 gttgtttctg ctgggagcat cgtccaagac aaccaagaac caatgatcga aataatcaag
    22081 ccgatattga tcagcgctgc tccccaattt cccacgattc cttttaaaat atttgccata
    22141 gcaggttgtg aaatagttgc taactcttct tgagataaga cgccgtatgg caagatcgaa
    22201 gcaaggatat agataaacaa taaacaaagc agaccaatga tcgatgcttg ctgggcttct
    22261 gaacgtttct tcgcgcggtt ggctaaaaca cttgcgcctt cgatccctac aaagacccac
    22321 atcataacca tcaaacaacc tttaatctgc aaccagatcg ttccttgctg ccctgttcca
    22381 ttcactagat tatcagatac gttaccccaa aaatcagcag tgaatatatt cactttaaat
    22441 gcgataaata caaatattaa aaataggaat aatggcacta atttacagat cgtcacgatt
    22501 gtattgatga cgctcgctcc ttcaactcca ttgtttacta ataaagtcag accccaaata
    22561 aaaatacttg caaataaaat agaaggaaca ttttgtccac ctttgaatat cgggaaaaat
    22621 tctcccatgg cgctcatcag catcgttgca aaagcaacat ttcccagcca cgcagacaac
    22681 cagtaacccc aaccactgat gaagccacct aatttaccaa atgaagcttc cgcgtaaccg
    22741 aaaataccac catctagatc tggacgcttt tctcctaaat gattcagcga taataccaaa
    22801 gctaaaatac caatcccaac gattatccat gaaaggatcg ctggccctgg agctgcagaa
    22861 ctagccagat cacttgtgat cccaaacaca ccgcttccga ttgacgaact gacaaccaga
    22921 gcaaccagtc cccatttgct aatacccgtc gatttctttt ccatttccat caccctcact
    22981 tataaaattg aacaaatcac ttgcaaaaga ttcccttcta ttttgcaact gctcttgctt
    23041 aacaaaaagc aactttattc atattattgg atattatgaa ctttttacac aagtatttca
    23101 agtataaaat gaataaaaat taattttatt gcaaaaaaac agctatgcta tccaaaagaa
    23161 aaggtgtttc ttttgttagc catagctgtt ttgattcatt taatatcgct taaacgtatt
    23221 gtaaaacttt tgtttcttga tatgcgtggt cgatcaaacc accacccaga cattccattc
    23281 catcataaaa tacaactgct tgtccaggag tgattgcacg tacaggttcg tcgaatacga
    23341 cttctgcacg attatcatcc aataagcgaa ctgtcacagg aacgtcttgc tgacgataac
    23401 ggaatttcgc tgtacacttg aattcttttg gcattggttc attcgtcgta aagtggattt
    23461 cacttgcatc caagcttgtg gcatacaatg caggatgatg gaacccttgc cccacatata
    23521 atgtgtttgt cgctagatct tttcctacaa caaaccaggg ttcttgcgaa tctcctccgc
    23581 caccgatccc tagtccttga cgttgaccaa tcgtataata catcaagcca gcatgttgtc
    23641 ctttgacttc accatcaaga gtgaccatat ttcctttttt tgctggcaga tagttgctca
    23701 aaaattgttt gaagttcttt tcaccaataa agcagattcc cgttgaatct tttttcttag
    23761 cagtcgcaag gcctgcgcgt tcagcaattg cccgtacttc tgatttttcc atgccgccta
    23821 gagggaacat cgtttttgaa agctgttctt gtgataattg gctaaggaag taagtttgat
    23881 ctttattatt gtctactcca cgaagcatgt ggaccacgcc gttttcatcg cgagtgactt
    23941 gcgcataatg tccagtagct acataatcgg cgccaagatc cattgcataa tccaagaatg
    24001 ctttgaattt gatttctttg ttgcacatca cgtctgggtt aggtgtacgt cctgcgcggt
    24061 attcggctag gaaatattca aatacacgat cccaatattc tttttcaaaa ttcacagagt
    24121 aataaggaat cccgatttga gcagctactt tcgctacatc cttataatct tccgtggccg
    24181 tgcatacacc gttttcgtcc gtgtcatccc aatttttcat gaatattccg atcacgtcgt
    24241 atccttgttc ctttaacaaa agtgctgtta cagatgagtc gacaccgccg ctcatcccta
    24301 cgacaacgcg tgttttgctg ttgtctgtca tgatatcacc atcatttcaa ttagattctg
    24361 aaacatctac gagttgaagg tcgcaacttt tcagtatttt acattatacg tcatatttct
    24421 gaaaatttca tcccttaatt atcaagtcgt aaaaatactg ttctactgga cctattcagt
    24481 acacaaacat gatagtcaac caaaaaatca ctcatctttt gagcattcga tataaaaaat
    24541 tggcatactc ataaaaaggg ccagcctttc ggcaataagg aataatttta taaattgtga
    24601 aaaactattt ttcaaaattg cttcttatcg cttaaagctg cacgtctcat ttacaacctt
    24661 ttctttttag atgttatgag ttgctcagat aagtgatttt gtttactcta agaaaaaagg
    24721 gaggcaaaag atacatgaat tactttatca gtgatatgca ctttttccat gaacgcttat
    24781 taggaaaaaa cgactttgct ccgcggctct ttgcaaatgt ggatgaaatg aaccaacagt
    24841 tgatcactag ctggaatgca gtaataaaag aaaccgacca tgtttatcat ctaggcgacc
    24901 ttgctatgca tccgcaatat gaaaaagcaa atgcagaaat tttggaactg gtcactcaac
    24961 tgcaaggaac aatccacttg atcaaaggga accatgacag tcgcgctttt ttccgttatt
    25021 tagaaaaaaa tgatcctagg cttactgacg gaagtcccaa attttatttt tatgatgtag
    25081 gtgccatcgt taaattcgac catcaacaat attacttgac tcattatccg atgttgctcg
    25141 ggcctaacga cactatccgc aatcttcatg gacatatcca tcattacagc gtacctatcg
    25201 agaacgatgt caatgtaggc gtagacgcac cggaaagaga actcttaaag gaaaaactac
    25261 catttggcac tccgttatct gagcaacaaa tcaaagaaat ctatgaagca aagaggaagt
    25321 acgataggtc gtgaaaaaac tgccctatat caagtattaa acaaataaag ataattactc
    25381 atatttttca tttgagtagg cttattgcca caggaacatt aggatcagaa atggagttgt
    25441 aacaagactt ctgtcacaac tccattccat actattcgtc tttttgctca tggtgttctt
    25501 ttaccagttt tttctgtaat tctttatcat aatttcgggc agctgacgtt aaaaataaaa
    25561 cgattccgca taataaaatc ccgattccgg aaaacaagaa cattttttct acccctaaac
    25621 gatcagctaa tggtcctgca aataataatc caattggacc tgccaggttc atcaaagagt
    25681 tgaagttccc taagacacga cctaaaacat tcgattcata actctgctgg atcattgcca
    25741 tcagcagtgt attgaaatat ggcgttgcac aacctgctcc tgcattcaag ataagaaagt
    25801 acaagaaccc ttgactatcg tttggaacca atccgcttgc accgatcgtc aggcccataa
    25861 tgatgtaggc tataataata ggtttcattc ggtctttcca tgttcctata aaactgatca
    25921 gtgcaccgcc taagagcatt ccgactgcgt agactacttc cactgctccc gcctgtccaa
    25981 cggaaccacc aaagtaatct agcgtcatca atggatacag actgattgct ggcatgaaca
    26041 acagcatcac aaatgcacca ttgatcgtta tgtaccataa ccctttatta tccatcaatt
    26101 gctgcagccc aaattttgca tctttaaaaa tagtaagcgg aacttccaat atttcaggag
    26161 aaacttttgg gatcttcaca aaaatcaata agccgacacc aaaaactgcc cccaatacat
    26221 ctaataaaat aaggtaattg acaggaacga ctgaaaacaa agccgcacct agagctggcg
    26281 caatgatgaa attcgctgat tggaccattc ctaactgtcc attcgtcctt gttatgtgag
    26341 aagatggaac gattgtcggc ataatcgact ggatcgttgg catttggaat gtttgtgcga
    26401 ccgaacgcat aaacaaagac acaaaaacta gccaaatcgg gaaggattct gaaatcgttc
    26461 cgactactgc taaaataagc gcgaaaatcg ctacaataat atccgtgaca atcaaaagag
    26521 cttttttatc ccagcgatcc actagcggtc ctacaaatgg gcttagtaaa accatgggaa
    26581 tcattcctag taaagtagca aaactcaaaa ttgtcgccga accggtttct cttgtcaaat
    26641 accagatgat cgcatattgg acgaccatac tagtgattcc tgacaaaaac tgtcccgata
    26701 aaaagaacaa ataatttctc ttccaattcg ttaaaacgac cgactgttca ttttccataa
    26761 acatccctct tttctttttc attttgtata gcgtttgcac aacaatcatt atacgggatt
    26821 cttttttcaa aaaatacctt tttccctaaa taacaatcgt ttcaatttaa atttctttac
    26881 ttagaaacaa taaatcagca aaatattaaa aataacaaca tgtgttaaat ttaaaaataa
    26941 aaaagacaac taatttgcgt cgtccttaca aagttagtca tctaacaata ggccgtttca
    27001 aaactagttg tctttttata tccacagatc attcgacttt ttcgaataac ccacggctaa
    27061 tcatcccatc catcagaaaa taaaatttat cttggctcat tttctgtttt ggatctttga
    27121 aaatattgac tgctttcaat ccatttgctg tagtgatcga cattttcaat gtttgaatat
    27181 cttttgatac agtgattttt aatttgaatc cttctttgct ttgcggcgtg gcatctaatg
    27241 gtcggatcaa ttgataattg cctccgtttg tttctgtaaa tccattatcg cgcaatgtat
    27301 atctttttgc atcaggatgc aaacgatagc taattggtgc gcccagtacc gttgctgttg
    27361 tttcaaatgc catgattttc acctcgataa aattagtttc tctttattat aaaagtttat
    27421 cctgattttt actaggtttt tcagttaagt tccctaaaaa atgaaggcgt tctctcagac
    27481 atctgttcat ataagaatga cttcactagt tattttttga cagcacgaac taattgatca
    27541 aggaaatact ggatgtcgtc ttcggtattt cccaaaccaa agctgatacg gatggattct
    27601 tttagtatcg gtgtatcttc tccatacatt gcttctaata cgtgtgaagg ctcgatattt
    27661 cctgctgtac aagctgaacc agtagaaata gcgaccccat taagatcaag cttcatcagt
    27721 agcaaatcat ttgcttcgcc aattaagcga agattcaaca catgagggag tttattcgtt
    27781 aaatcaccat ttacttgata agggatttcg tttgcttcta atgtatcaag aatcaattgc
    27841 tggaatcctt tatatttgtt gttacgtgct tcacgttctt caggtgacag cagttcaact
    27901 gcttttgcca taccccagat ccctgctaaa ttttctgtac ctgcacgccg cttttcttcc
    27961 tgctccccgc ctaaaagcaa tgcagggata tttactccgt ctcgtttata taagaaaccg
    28021 acgccttttg gcccattgat tttatgagca gagatactta ataaatcgat tcctaattct
    28081 tctggatgga tcgtttggtt tccgtacgct tgtaccgcat ctgtatggaa ataagctgga
    28141 tgatccgcta aaagttcacc gatttctttg attggcatca aatttccgat ttcattgttc
    28201 ccgtacatca cagagaccaa gatcgtatct tctcgtaacg cttttttgac ctcttcgatc
    28261 gacagattcc ctttttcatc tacaggcaga tatgtaacgt caaacccctt tgtctctaaa
    28321 tatttcatgg tattaagaac agcaggatgt tcgatagctg tagtgattag atgtctgcct
    28381 tctttttgac gcgaaaacgc tgtttctaag atagctgtat tatccccttc tgttccccca
    28441 ctgttaaaga tgatctcgtg aggttttgct tgcagactgt ctgcaatgat ttgtcttgct
    28501 gtttccaatt tttcatgcgc gtggcgacca aatccatgta tgcttgaagg atttccaaac
    28561 gtgtcttgca tgattgccgt catcgcttca attacttgtg gatgcatggg ggtagttgcc
    28621 gcatgatcca gataaatagt tttcacttca ttcatccctt ccaaattatt gctaggcaca
    28681 ttctagcata aaatagcgaa caaaccaaag aagagctcat ttttgtctag aggacgtttc
    28741 atgggcatag aaaaaaggag ctgtgacaaa agtcttgtcg cagctccttg aagctgctca
    28801 cttctgtcac aacttctttc cctctttttt ttgtttattc gaattctttt tcttccagac
    28861 ggaataacgg actcatcggt gtattttcat ggatgcgttt gaccgcttca cccattaatt
    28921 ctgaacatgt aacgattgtt aagttttctg gatgacgatt ttcttctgtg aaaacagaat
    28981 cagtgataca aatatctttg attggtgcat tgtctaaaat acttttcgca tgatcggaca
    29041 ataatccatg tgacgcacag gcatatactt cttttgcgcc gttttctttt aatacatttg
    29101 ctgctcgtgc aagcgttgct cctgtgttca agatatcatc taccatgata catgtctttc
    29161 cttgaacatc accgatcaca taaccaccct cagttgaagc atcatctgca tgatctacga
    29221 tggctaatgt gctgttcaaa tattctgaaa gactacgtgc acgctgaaca ccgctgtttt
    29281 ttggcgaaac aactacgatg tcatctcccg tcagacctaa ttgacggtaa taatgagcaa
    29341 acaacggcat cgtaaataaa ttatctacag gaatatcaaa gaatccttgt acttgaaccg
    29401 tgtgcagatc taaagtcagt actcgagttg cccctgcttc ttcgatcaag ttagcgatca
    29461 atttcgcagt gatcggttca tgaggtttcg ccgtgcggtc ttgcctagca tacccatagt
    29521 aaggcaatac gacattgatc gttttcgcac ttgctcgctt cattgcatcg atcatgatca
    29581 gtagttccat atagtaatca ttgacaggtg cattcgttga ttgaacgata tacacatgat
    29641 cccctcgaat actttcttcg atattgatgg aaatctcgcc atcactaaat tgcttcacga
    29701 cgcttttacc taattctgtt ccgaacactt tcgcgatttt ttcagctaat ggacggttgc
    29761 cattcaaact aaaaatcttc aacgtgtcat cctgatactt attactcatg tagtcctccc
    29821 caaaacttct ctattcttcg atagtttaac atatttttcc aagattttct gttaatttca
    29881 gcaaattctt aaggaagtga aactgttccg ttactccaac tggcaggaat gactttatga
    29941 taatcgcccc agttgtcgtg tactttgtaa taacgttctc ctgtttcagt ttcaaaataa
    30001 gcgtacgctg tcacccaatg attgccatat gtactgccaa gtactttcaa aataccaatc
    30061 atcacaggtt tgccttcacg tatacgtttt gtagctcgct gccatgaacc tagaagagtt
    30121 gctcgtgcac gctcatgatt gccattcttc tttaaaaaac tagaaatccc tgtactaacc
    30181 tgaaaaggaa tcgtaggaag tcctagtggt tggatctggc tacgcaatgc cttcgtcaaa
    30241 accaacgaat cagccgtatt tttcttgcga atctcatttg gaagcatcca ccccttacga
    30301 tagtcttgat aataggcaag gagaactgct gccgcgtaag taccacaaag atagcctgag
    30361 ggggtagccc atttcttata cctcaaaaaa cgttcttctg gcaatcccac ccagcaattt
    30421 gcgagttcct ttcctgacct tttttcttcg atcaaaacgg gaatatcttc ttttctgttg
    30481 atccatctag ataactcgat tggctcttca ttgatcagct tccatttttt acaagaaaga
    30541 ccctcttgat aagtatattt tgctggagaa tattctaccg gatgtcctga ttgatcgata
    30601 atcacataac tttgatcttc aaaaaccaga gctaaaaaaa gcaatttact ttttcttgac
    30661 tgcagctgct gagtgctggc taatttttta atttttttat ctactttgaa atataaaggt
    30721 ttatccacta cttgattcag ttgggatcgc ctcctttttt tcattataag tgaaggattg
    30781 aaaaaaacaa atcagtttga ttgttcgaat tttcaatttt ttatgttcta tttgttaatt
    30841 ccttcatttt gtaagataat gggcacacga aacaaaaaag gagcgatcga tgaatgaaca
    30901 aagaacgatt gaaacaagat ttctatgaag cagtcaatga agaatggcaa aaaacagcaa
    30961 aaatccctgc agataaacca gctacaggcg gtttccaaga tttagttgac gggatcgacc
    31021 aacttttgat gggagaaaca gacgatctgc ttgcccatcc agaaaaagtg tcttcagaaa
    31081 aaatgaaaca tttccttgca tattatcaac tggcaaacga ttatgaccga agaaatgagt
    31141 tgcatgctca accgcttcaa cctataatcg aaaaagttga atcattgcag tctttcgacg
    31201 aattgaataa gcaatttcca gaatggctgt tggatacatt accattgcca tttaatctgg
    31261 atgtagatgc agatatgaag aatgcgcaaa cgaacgcctt ctttgcttat ccgccttctt
    31321 tattccttcc agataagacg tattatgcat cagaccatcc caacggtgca caactaatag
    31381 gcgttttctt tgatatggct gttcagttat tagagcttgc cggcaaagaa aaaagtcagg
    31441 cagaagcaat cacggaacaa gcagtgcaat ttgataaatt gattgctcct catgtaaaaa
    31501 gtgcagaaga aaacgcagat tacagcaaaa tgtataatcc gcaatctttt gatacattta
    31561 ttaaatacac agataaactg gacttgaaaa aacttgtcac gggtttgatt gggacaactc
    31621 cagataaagt gatcgtaact gatccagtgt acttcgatca tttgaatgac atcctgacag
    31681 aagctcattt tccgttattc aaaagctgga tgatcattaa aacagtacgt tctcttagtg
    31741 gttatctatc agaagaattc cgccaagtca gcggaatcta ttctcgcgta ttgtctggaa
    31801 cagacgaagc aatgcctcag aaaaaagcag cgtattacct tgcctctgga caattcgatc
    31861 aagtagttgg cgattactac ggaaaaaaat attttggcga agctgctaaa aaagacgtag
    31921 aacaaatggt gaaaaaaatg atcgccgttt ataaaaaacg tctagaagaa aatgactggc
    31981 ttggggatgc aacaaaagaa aaagccgtca taaaattaaa taacttaggt atccaagtag
    32041 gctaccccga taagatccca gcattattcg accagttcaa aacagtacct gcagatcagg
    32101 gtggtacttt gctttcaaat actcttgttt tcagcaaact cacattaaaa gatcgtttca
    32161 gcaaatggaa caaaccagtg gatcgttcag aatgggaaat gagtgctgac acggtcaatg
    32221 cttattatca tccatttcgg aatgtgatcg tctttccggc agcgatcctt caagcaccat
    32281 tttacagtct agaacaatca agctctgcta attatggtgg aatcggagct gttatcgctc
    32341 atgagatttc ccatgcattt gataataacg gcgctttatt tgacgaatat ggcaacttga
    32401 acaactggtg gacagaagaa gatcttacac aatttcaaaa caaagcacaa gcaatgatcg
    32461 aagaattcga tggtctgcct tttgctgacg gaaaagtaaa cggaaaatta accgtttcgg
    32521 aaaatattgc tgatgcaggc ggtctaagct gtgcattgga agcagcaaaa acggaatcag
    32581 atctttctat cgaggaattc tttgtcaatt gggcaacgat ttggcgtaca aaagcaaaaa
    32641 aagaatacca acaactttta ctgcaaatcg atgtccatgc ccctgctaaa ctccgagcaa
    32701 acgtgcaacc aaaaaacctt gacgaattct atgaaacatt tggtatcacg gaacaagacg
    32761 ctatgtatct agcgccagaa aaaagagtgc gaatctggta agaggttgtg ccagaagtgg
    32821 tcagcttcaa gaaataagaa ggaatttggg aaaatcgctt ctcaaatttt tgtgacattt
    32881 cggcttattt ttcgaagaag ttacttctgg aataccatgt attagaggtt gtgaaaaagc
    32941 tgcttagttt caagtagtaa gaaaaatttt gtgaaaatgg ctttccacat ttttgcataa
    33001 atttgactta ctaccgagaa actggctttt gaacaccgtt tatcgagagg ttgtgaaatc
    33061 agcgtcctga cctgagtatt agagaaaaaa acggaaaaat agttcctcat attttgccgt
    33121 ttgttgagct aataccgagg gtcagctgat tgaacaccgt ttagtggaga gattgtgaaa
    33181 aagctgtcct gcatcaagta ataagaacag ccaatcaaaa gcagcaagga gaacctcatc
    33241 tataaaaaga tagaaggaag tgagacatct attggtctca tttccttcta tttttacttt
    33301 cctcagtcaa ttgttttaca tcataaaagg agaaattcca cttatttttt tcgctgtttc
    33361 atgacataaa caactacacc gattaacaaa attcctccag ctaatgctag caacgggtta
    33421 ttaaattctc ccgtttttgg gagtccttga gtgccatttt tttcactttc ttttgctgtc
    33481 ctatccggat gagaaggttt tccatttggt tttccatttg gtgattcatt tcctggattt
    33541 tctctttctg gtttgtcatt tcccgggggc acatcggcag tttctcgcac agtgatcgct
    33601 ttttgctggg taacagattc tgtatgagca gaccattttt ccccatcaaa tatttccgaa
    33661 cgataagtga cttctagcgt atattctcct tttgaaggtg ctgaaaaaat agcggtatga
    33721 ttagaaaatt gagcacttga ctgtgtttca acgatttgga tcttttctgg gaaataacgc
    33781 atatcttcaa agacaggcgt tccttgattt ggtgtgaaag ttgacacaaa aactgtaaaa
    33841 ggcttcagtt caatgatatt tccttctgca ttctccgcca caccatttgc ttcttgtata
    33901 ataatcggat tattttgttc atttggtttt gtctgatttg gttcttgtac gattgcatac
    33961 tctggatatt ccgaagacgg tgaggtttct ccgtcaaaaa tagcacctac agataatgga
    34021 agaccatcca ctctttcctt ttctgtttga cgattccccg gtttaatctg catcagtcca
    34081 tgaacatgga tacttccatt ttcatagcgc gtaacaggct gctgaccttc tttcgaacat
    34141 gtatttagag actcgatcca atcttttgat atggtatgtc caagagtatt ttctgattgt
    34201 ttctctcgtt cattccaata gtagttcaac gagtggtaga tttgatcttc gttttgattc
    34261 aatgggcgga tctgctctct catctcaaga ttctctgttc ggaaagataa gataccagaa
    34321 gcatggtagt ctgtgtttcc tgctgaagag gtataaagag ccacattata tgaaccgtta
    34381 ttaaaacttg tactgctatt gacgataata tctgttccgc tattggaatc gattcctttc
    34441 gcaagatttt cgaaagacaa actattgatc aattcatgag ggcccgttaa agaagaaccg
    34501 cccattttga atccattacc gttaccttct ccctcaattc ctggtaccca gccgtttcga
    34561 taagcaactg agtttttgat gattactttt ccgatcgaac ctgtttcatt tttagcaaaa
    34621 aggtcccaac cgtcatctgc attatgatac gcgatacaac cgtcaaatac atttccttct
    34681 cctattgtca gtttggccgc aaatccatcg gcatcttcaa atcctggatc ggcattttca
    34741 aaagatgtac agttcttgac taagttatgc gcgggccatg cttcgaaagg atcatttgag
    34801 ctaccgctga tttggattcc tgtatttcca ttccgataag catgaatctc ttcgatcaaa
    34861 ttatgatttc cagaaatctg taatcctttt tgcatagctg cactatttgt aatgtcaaac
    34921 cctcgtaaac cccagtagtg actgaacagt gtcatcccat ttccagttcc tttaaaatca
    34981 agtacaggac gcttgttttc tggatttttc tctgcaatca actgaatcgg gttttctctt
    35041 gtcccgttaa cgccttttgg aatcgtcaat cctctttcga aggaataagt tccaggcgcc
    35101 atgacgattg tctgcccagc atatgcgtat ctcaatgctt gtgtcagaga taatggattt
    35161 tgtctgcttc cgtttcctgc atctgaccct tctggtgtta cataaactgt cgaatattta
    35221 ggtttacggt agtccactgt atgaagaatc tcttttgttt cataactagt catctctacg
    35281 tattcttctg gcggatagtt tttatctggc gtaaaactga aagtaaattc gttcgttcct
    35341 tccatcagtt ttgtctgata atcaaattcc cgattcgcag tgatagccaa gttttttgcg
    35401 ttcttaagca cctctcctgt tgctttattt ttaattgtca ataatccgtc agcatttgct
    35461 ctaaaggaca tagtatgatc ttggtttcct gaatgattac tagaggtcac agtgtaaatc
    35521 ggatcaatca agcgagtagg tctttcttgc ccaggaggat ctgtcgcagg atctgagtaa
    35581 gcaacttcga ttccttctac cttgatcgtc atattccttg ctgcagcaaa tcctgcataa
    35641 ataaccgatt catctgaagc gtataatttt tcccagtcat acatgatgtc agaagccatt
    35701 tcttctcctg tttcaggatc gtaataatat gcctctaaac cagtattcgt ttttttcatc
    35761 tctacgtcca gtttagtaat cgagttcgtg gccggaaaca gattcccatg accgtttccg
    35821 aatatattat aagaacctgt ttctttttcc aagaaccctg ctgaagtctc taaaggtgtt
    35881 gtttccactg agacactccc aggagctggc gcagcttgcg gatcttcaga tgatattcct
    35941 gtgattgccc gtgtccccaa tccaagtcgc atcgtatatt tgctgccgct cgtgttgacc
    36001 acttcttgtc tatccgagtc ccagacatat tcgatccgac tgcctagtat ggcaaatgaa
    36061 tttgaataaa atgcctgccc aaacaaatga ttttttggta ctgcgtctct taccattaaa
    36121 gcaaatcctt cttgggcatt cgtataggtc caagattcta tttcgatcgt cgcccgcatc
    36181 ctaaagttga tgtctttggg gattgcttga taataatagg tcattccatc tgatccgctt
    36241 gactggaatt ttccgccgtt attcgtggaa gagagccgca cgccgtcttc taatggttca
    36301 atggcatacc tactagcact tgtgctttct ccgaaaacaa cccctttcca ctcttcattt
    36361 gcttcttcag attgcttttt tgtcttttca ttttctgatt ctttcatcat aggatcattt
    36421 ccttcttccg tatccatatc ttcactagtt cttggtattt cctccattgt tgacatccct
    36481 tcaatgttca cgtgtgcatt ttcttcgttc acagactgtt gttcgttagc agcaacttca
    36541 acgacagaaa ttaaaaccgg ggtactgagc ataaggagat tcatcattag cgcactgtat
    36601 tttatacgtt tttttaacaa gtgtattcct cccgtaacaa attagaaagc gtttacttaa
    36661 agtattacct aatttatttt tactgtcaat aaaaaacaaa attataataa gtgtatttta
    36721 taaaaagaaa aaatataact taagttgttg ataataaaga gatttattaa cggaaaaaga
    36781 ataagttttt ttaaaataac gtataaaaaa gagcataaaa caaagtcgtt tgttttatgc
    36841 tcttctttta ttattttgtg ctttttattt tatcaaatca ctttgttttt ttataaaatt
    36901 tctgcgccta gtgtggactc aaaatgtctc aatgcccatt catgtccaac gggatcgaaa
    36961 cttgccacgg aatctttgaa aatatggagt ttgtatccta gattatacgc atcgactgct
    37021 gtatgcaaca cacagatatc tgtacatacg ccggtcaaat aaatatctgt gatctggcgt
    37081 tctcgcaaac ggatgtctag atctgttcca ctaaaagcgg aataatgccg tttgtctatc
    37141 caataaacat tttcctcttg ctcatagtct tggtataaac tttttaaaga gccgtacaat
    37201 tcccgtccac ccgttcctat cacattatgc ggaggaaata agcggttctc cggatgaaag
    37261 gaatcttttg gatcatgcgc atcaatggca aaaacgacaa aatccttttg atcaataaac
    37321 ttctttgtat gagcagtcag ctttttttca attacttgtc ctgctgtccc agtagtcagc
    37381 tttccatcct ctgcaacaaa atcatatgtg tagtcaatcg agatcaatgc gttcatctta
    37441 gtcctcctta tacgagatgt ttgacaaatt cgatcaccat ttctgctgat cgtttgcctg
    37501 cgtcttcgat aaattcgtca aagctttgtg tcgccgagtg atctgctgtg tcgctcattg
    37561 cacgtacgat caaaaatggg atattgaact gacgagctgt ttgtccaaca gcggctcctt
    37621 ccatttcgca agctaatgct tcagggaaat tcgttaagat ttctttgatt ttatctggcg
    37681 agtcgacaaa tgtatcacct gtaacgatca atccttcttt tgcgttcaaa ttcgttgctt
    37741 ttgctgcttt aaccatttca ctgcgcaaat atgtgcttgc ttcataatat agaggcattc
    37801 ctggtaattg tcctgggttg tagccaaatc ctgttgcatc tgcatcaaaa taagctactt
    37861 tgtctgatat aacgatgtca cctacttgta agccttcacc gattccgcca gctgaacctg
    37921 tattgatcac catgttcacg ccgtattgtt ggatcaacag acttgtggta atagatgcta
    37981 agacttttcc tataccggaa cgtacgacga tcacttcgtg gttacctaat gaacctgaaa
    38041 taaataaagc gcctgcgcgt tcccatgata atggttcgct taggttctct ctcaaaattt
    38101 ttacttcttc ttccattgcg ccgataatac cgattttcat ctttttaatt ccttctttct
    38161 atataaatgc gattgctaaa aaaaccacac ataacattag gataacaata ataagagatt
    38221 tcatcaaaaa agagttccat ttggtcgtct tttcccgttc gctgatcctt gttttcgtga
    38281 ttggtttatt ctttttgctt tctttacgat aaaagctggc aatctttttt tcttcttgct
    38341 gataagctgt ttctgctttt cgctgttttt ttaatgactc gcttgcttgt gcttgttgtc
    38401 gcttgcgaag ttcgcttcgc gtaataagcg gtcctttggc cataagccga tccctctact
    38461 ttcctttggt caatagttcc cagtattgaa tcgcataaat cgtctttgca tcacaaatca
    38521 actgatcttt catcgcttgt tgcgcctctt caagagttag atgatacaac tccagtactt
    38581 cgtcttcatc ttgtgctaaa ggattctcga ccttctctac cccttgtgca tgataaatat
    38641 gaaggacttc atttgcaaaa cctggtgaaa gatacatcga agtaagatgg ctcagcgact
    38701 tcgcacgata gccagtttct tcttctaatt ctctagctgc tgtcatttcg ggattttgtc
    38761 cttctcctgg atcgattttt ccagctggaa tctccaaaat cactttttct aaaggttttc
    38821 gaaattgttt gacaagaagc agacgatctt gttcgtcaaa agcaatgatc ccgacaccgc
    38881 caggatgaaa taccagttct ctcttcgaag ttcctccatc tggcaaacga acttcgtcta
    38941 cagcaacatc aataattttt ccgctataga tttctttgcg actgattgtt ttttcttcaa
    39001 attgctcatt gttcagcata tcttctcctg ctttctttgt aatcataact gctttttttt
    39061 catttttcaa tttttctcaa aaactcctta ttagtttacc atagataaac cttagatttt
    39121 ctctaaatat agaaaatcct tctttagtcc gatactcttt ctagtagtcc gtatgctaaa
    39181 ataagtatgt aatcgaataa cgaaaagaat tcaatactca acgataaatg acatggaggg
    39241 tttattatga taaaacgaaa ttggccgtgg cttctagcat tggtcttgct atttctcttt
    39301 gtccgaaaca atagtttttc ttttgttcta ggacttttat tgataggtgt agttttagtt
    39361 ttgtggggac tctttggttc gcgcaggaaa aagggaaaac aagaagaaat tcctgcactg
    39421 actgacgaac ttgaaactca ctatgaaaaa agcggtatga cagcgaatga aatttcattt
    39481 tttcgacaaa caatgaatca aacaaaaaat gagatcaatc aattgcaaat gaatatgcag
    39541 cagacagcca aacttaaagc aatcgatctc cgccatgatc cagtcaaagc agcgaaagct
    39601 ttattcaaag aattggtgaa agaaccaaac cgactgcatg aagcaagtca gtttctttat
    39661 acgcatctgc cgaatctagt tgatttgaca aataagtata atgagatcaa tgaccatgag
    39721 atcaaaaaca aacagaccta tgaaaaacta gaagaaagcg cgcagatcat cgaccagctg
    39781 tcagctttaa tcgcacaaga ttatcaaaaa ttcgttgctg aggatctaga tgatatcgac
    39841 gtagagatat ctgttgcgaa acagagctta aagcgtgata ataaatatga accagaacaa
    39901 aaggattaac tcttttgttc ataaaagaag actcggaaca ttcagatgat gtctggctgt
    39961 ttcgagtact ttttctaaag tagagacatt catgtaagaa tcgagaacag attcaacaag
    40021 gaggaatttt ttatggatca taaaccgact gaaacaaaag atgttacacc agttaacgat
    40081 acacttgaag atttattgaa caacccgttt tcaactccaa cagatacgct gaccacttca
    40141 caacaagcgg agattgatca gctccaagag caacagacag ctgctcgttt aattgacaaa
    40201 ctaccggcgg aacgccaaga acaagctaaa cagctagcag caaaaatcga tgctaatgat
    40261 gcacagtctg tcatcagcta cggatcggct gcccaagcaa aattaagtga attctcacaa
    40321 tcgatgttga atcatgtgca agcacaagat attggtcctg taggagattc cttaactgaa
    40381 ctgatgtatc gtctccaaga agccaatcct gacgaactgc gtgccggaga agggaacatc
    40441 ttccagcgtg tcttcgggaa ggtcaaacaa tcgatctatg aagtgacagc aaaatatcaa
    40501 aagattggtg cacaaattga caagatttca gtgaaattag ataaagaaaa agacggtctg
    40561 ctaaaagata atctgatgtt agaacagctt taccagaaaa acaaagacta ctttgatgca
    40621 ctgaatattt atatcgctgc tggagaattg aaaatggaag aacttcaaac aacgatcatt
    40681 ccagaagcga tgaaaagagc agaagaaact ggcgatcaga tggatgttca gattgccaat
    40741 gattacacgc aattcctaga tcgtctggat aaacgtacgc atgatcttcg cttagctaga
    40801 cagatcacga tccagcaagc accacaaatc cgtttgatcc aaaacacaaa tcaggccttg
    40861 gctgaaaaga tccaagcatc tatcgctaca gccattccgc tgtggaaaaa ccaagtcgtg
    40921 atcgcattga ccttgcttcg tcaaaaagat gcagtaactg ctcaacgcca agtctctgaa
    40981 acaacaaacg acttattgaa gaaaaattca gaaatgctga aaatctcagc catcgaaaca
    41041 gcgaaagaaa atgaacgtgg aatcgtggat atcgagaccc ttcaaaccac tcaaaatgat
    41101 ttgatcgaaa caatccaaga gacattgcgt atccaaaaag aaggaaaaga aaaacgtcgt
    41161 catgcagaag tagagcttgg acacatggaa gaagatctga aacaaaaact gttggattta
    41221 acccaataga tcacctttcg cttgttacta aaacaaacgc ccaacaacag tttattacaa
    41281 ctgttgatgg gcgtttttct tatctttctt ctaacttctc cattggagtc tatttctcat
    41341 tcatcttgct gtgtccatag ccatagccaa aataaatgat cagtccaata accattacga
    41401 taccgaacac gatccatgtc acagtttgca gacgaagcat caatacaata caagtcaaga
    41461 tggagacgat tggcaatagc ggaacgaatg gcactttgaa ttctccagct tttggtttgc
    41521 caaacatttt tctcaacttc agtaagccta gactaacaag catcaaatag gcgagcgcca
    41581 tgatattcgt tagttccgct aatacattca atgggaaaaa tccggcaaag aatacagctg
    41641 ccacaccagc ggcataagtc gcttttttcg gtgtacgatg cttttgatcg atttctgtca
    41701 taaacttagg aagtaagcca tccttgctga cagcaaacag taaacgagca agtgaataca
    41761 tcatcgagat cgttaccgtc agtagcgtta aaattgcccc tacagcgatc accgcgccca
    41821 ctgttccatg tcctacatac ctcattgcaa aagccactgg gtcattgact cctagttcag
    41881 tatacggaac gattcctgtc aagatcagcg tcacgatgat gtaaaggaca gtagcaatca
    41941 gtatcgaacc aatgattcct cttggcacat ctttttgcgg gtttttgact tcctcggctg
    42001 ccattgcaac ggcatcaaat cccaagaaag caaaaaacac aagagctgca ccatctagca
    42061 caccggaaaa accaaaaggc atgaatggct gccagttatc aggtttaacg aaaaatattc
    42121 ctacaatgat aaacaacgcg atgataccaa atttgacata taccatggca ttattcaaac
    42181 gtaatgcttt cttggcttca agcgacacga tgtaagtgac gaatatcact acgagcaccg
    42241 caatcaagtc aatgtatgta ccattttccg gattgtaccc gccagttaat gcttgtggaa
    42301 gttcggcccc taaacttttc aaaaaacctt gcatatagcc agaccatccg gaagcaacag
    42361 aggaaaccgc tagtaaaaat tccccgatca acaaccaccc tgtcagccag gcagcaaatt
    42421 caccaaatac aacatacaga taggcatatg gcccaccaat cactggcact cgcgaagcaa
    42481 attctgcgaa acttagtcca gacaaaatga caacgattgc agctaagaca aatgaaagtg
    42541 acagtgctgg accagcattt tgattggccg caacccctgt caccacaaat attcccgtac
    42601 cgatgatcgc accaattcca agcatgatca aatcagacgt ttttagttct tttttcatct
    42661 cactcgtatg attgagattg atctctttct tacgaaacaa atccattgaa gttcctcccc
    42721 attttaggtt ggaaaacagc gattaaactg ttttcatata ccctaaaagg ataccaccta
    42781 aaatgactaa aaggcaacca acaataacat aaatgagctc ttttttcgtt tttctttcgc
    42841 caaggatgaa aatcccacct aaagtagaga taatgatccc catttgtgaa agtgagaagc
    42901 caacagctaa accaagtgat ttgactgtta gaagcatgca gatgtttcct aagccccata
    42961 ataatccaca gatcatgttt ttccacacaa actgatcgac tttaacttta cggaaagcaa
    43021 agaagcttgc gcctaagatc attccgatac tttgaggtaa gataattgac atcgcatcca
    43081 aattcgccca gttgacgatg atggtatata caccataacc aatcgtagag aagatcaatg
    43141 ctctaaaacc cttaccaaaa tcaagcattt tattatctgt atctattttt ccttcgtcat
    43201 cttgtcgggc agtcaaatac gcaccgctta caagaagagc taaagcaata ataccgtata
    43261 cataatcttt cgtttgtgtc cattcatgga agaaaacggc tcccgcaatc gtattcaaaa
    43321 ttaattgaaa accagtagac aatggtagcc ctacagaaac acccatgtac ttcattccat
    43381 ggaactgtcc attttgtcca acactccaag ctaagccgga taaaaatccg atcaagaaaa
    43441 tccaaggggt gattacagga ctaacaacga aaaagacaac taaagaaaac agaaatgcac
    43501 cgatcgtcat tcctagtgtt tgttgattgg catcgccacc aattttacca cttactaaac
    43561 caatactacc ccatgccaac atagggacta atgctattaa aatttccata accaatctcc
    43621 ttttatttca catgataaga aacttatcag cgaaaacgtt aacaacacaa gcactttttt
    43681 tcgtttctaa cgattttcta acgtttttca ttataaaaat ttaagattgg gaaaaataaa
    43741 gtcattgttg tcccttttcc ttcagttgat tcagccgtca aatggatacc taatttttcg
    43801 gccagatttt ttgctaaata aagtcctaat cctgttgaat gtgttttgct aagtcgacca
    43861 tttctcccag taaatccctt atcaaagatc cgtttcaagt cttccgctgg gataccgatc
    43921 cctgaatctt taactgaaag gctgactcct tgcgcttcct tttctatgct cacaatgatg
    43981 tttccgtgat ccggcgtata tttgatggca ttactaagca attggttgaa gataaaagcg
    44041 atccatttag catcggtcaa aacagtatga tcctcaccaa cgatctcgta gcgaatattt
    44101 ttttggataa aataatttgc ctgactgcgt atcacaggct gaatgattcc ttttagtcca
    44161 tattcttgga tgaggtagtc ccgcgagaaa ctgtcgagtc ttgcataata caaaacttgt
    44221 tctacgtatt catcgatttt tgataattca ttttctagta atgtatactt ttgatcgtca
    44281 atatcgaact cgatcgaacg aactagcaat ttagaagcgg aaagtggcac tttgatttca
    44341 tggacccatg agtcaatata atctttttgg tcttgttgat tactgattgc tgattgcatt
    44401 acttcttggt gttcacggat cagctgattg atatattctt ctaccagttt ttcttcttct
    44461 gtcctcccac cactcaaata attttgcaaa ggggaagttt tttgtatgtc ggctaatttt
    44521 ccccaccatc ttctcttgct caagtaacca cccagcagat aaaaaatcag aaagacgccc
    44581 tcaatcaaag ctaaataagc aattgtgctc aaatcaactt tgatgtttgg cgccagccac
    44641 atcacaaaga tcgtcaatgc aataaaaaac agccatccca agatcaatgt ccactgatct
    44701 ttcaaatatt taaaaaaatt catcttaacc gctcatcctt tcagggaacg atatatccct
    44761 gtcctacttt tgtttcgata tatccttgga tacctgcctg ttcgattttt cggcgtaacc
    44821 gattgatgtt gactgtcaat gtattgtcat ctacaaatcg ttcgtcatcc cacagtgctc
    44881 gcagcaattt ttcccgactt aagatctttc cattgtgctt catcaaaata aataatagac
    44941 gatattcatt tttgctcaaa tcaatgatct ctccgttgat ttccatactt ccgttgtcaa
    45001 cattcaacgt aatgccattg tggctcatca tttcactaga taattctgtg tagttgtaag
    45061 aacggcgaag taatgcattg atttttgcaa tcagtacatc gatttggaaa tgttttgtca
    45121 caaaatcatc tgcccccatg ttcatagcca tgatcatatc catattcgta ttcctgctgg
    45181 agataaaaat gatcggtact ttcgatactt cacgtatttt ttgacaccaa taatagccat
    45241 ccaaaacggg aagattaata tctagtaata caagctgtgg ttcttctctt tcaaagtctg
    45301 agaacacttg attgaaatcg gtcgttccaa aggtttcaaa ttgccatttc tcaagttcct
    45361 ccatgatcaa ttgtcgaatg atctcttcgt cttcgactac catgattttt gccataaaaa
    45421 atccctccat ctttcttctt gctactacaa tatcaaagaa gcccttgtga aacaagttgg
    45481 cttacctgga aaatgtcatt ttagcaaacg ttaaaaaaaa ctggaaatat gcgatttaat
    45541 cgcgtttgtc tcttccaagc agcttaaatt tattatgtta ttcttaataa ttggtctatt
    45601 atctgttatt tttctttcgt tgtttttgtc ttttaaattt tcgataacac caataaccga
    45661 aaagcgttag cactagcaat atacctagaa ggagtataag taatcgataa agatggtatt
    45721 tttttgtctg ttctatcttt gaagagactt cttctgtaaa gggaactcgt cttcctgtca
    45781 ctagtaatcg gtgagtgttc acggtgtaag gtgtacaagt gataagtgtg actagatcct
    45841 gcttttctct aattgctaat gtatttgttt ttgttggaag gacaacaatt ttctcaacga
    45901 cttcataagc gagcatccga ccatacactt caagatagaa tcggtcatct tgctccattt
    45961 cttcaagatc cgtaaagagt tttttctcag caataccgct atgggcggta atcacggaat
    46021 gtgtactctc tcctccagtt ggaaaagagg tgccttgcag gactgtcgct cctctatcta
    46081 acaaaagatc attcgtttca tcaaataaag gcaagctaac attgattttc ggaatataga
    46141 tggctccgat catatgttgt tcgtaatatt cttttcctgg attttggaca tttctaaccg
    46201 cttgctcaaa aggatctttg acttgtccca ttcctgggat tactgatgta tgagcaagcg
    46261 tctgattttt cttttcatgc actgctaatt gttgctcatg tttttcttct ttatcgcgtt
    46321 caagctgctt ttgataattc tcgatccttc tttgatccaa ataattgctg agagcatctg
    46381 ctaaaaatgg atatgtaaaa actgcagctc cggcaagaaa catcactgcc ataaataatt
    46441 gtaataaaag actgatttgg gtttgaggtc tattacctat tttctttttc attttcttca
    46501 ccttcttatt taaaaaaact gcagcaacct ttataaaacc gattgctgca gcttattttt
    46561 gttgcttata cttcagactt cattttagat ttacggtacc agctgtacgc tccaatcatc
    46621 aaacttattc cgattagtaa gaatgccaag agcccgttcc caccagtagc aggaagtgac
    46681 ccgcgtttca tattcggtac tctttctggc cctggatttt ctactcctac gatctgaatt
    46741 tcttcattat agcttccata agagatgata aattcagtaa aggctgcctc acctgttgga
    46801 agtacataac cttctggcgc catggtctct ttcatttgat aggttccata atctaaccct
    46861 tgaacctcaa agtatccatt tccatctgcg acaaattctg ttgcttgtcc gcttgttgcc
    46921 cactcaattc tatctgctcc tgattcataa acagctaaag gcgactcccc tttataaaat
    46981 attgcgtatt cagaacggtc ttcattccac aattcgaatc ttgcatcttt aagcaattca
    47041 ctgcttgatg catcatattt ttcaaacttt cttccacctg ttgtgacttt ctctggcggc
    47101 tctactcctt cttcataatc aaattcatca tttcgtccga tttcaataac cgctgtgttg
    47161 ttatggaaat catccgcttc tagttctgtg gaaaccttca aattatacgt gatcgttaaa
    47221 tctcctccac gtgcatctgc taatctacct aaagtatcta ctttggctac tccattttct
    47281 tctgtcaaaa tcgtcacgac aaacccgttg cttactactt ctactatata atcttgtcct
    47341 tttgttaaaa tcgtctcgcc ggctcgcact tctatttcct gatcataata agttagacca
    47401 actgtaggcg tatctgtgat tttgtaattt ttgataacag cgcttccgtt ttcaaggaca
    47461 gaatcgatga aataaggaat aggtgctgtg atcgtgtaag aagccgtttt tcctctttct
    47521 agatcaatat aagaaatcgt ttcttgttct ccagcttcat taataatcgt tacttgtcta
    47581 aggtcgcttt cctctacatt catctgttta tctgcttctc tgatttcgtt tttcggatac
    47641 aaatgcacat ctgtattgta tgtgtctttc catgtagaac catcccacac gccatccacc
    47701 atatcaggca tcatcactgg catcataaca accattggag acgcgattct ggaaatatga
    47761 gctggcgaat aagtttctaa gaaaagatag actttatctc tagaagaagg tgcttcttcc
    47821 gtcacttgga cggtagagaa taccgcttct ccagcttcat tcgttgtttg tttgtcaata
    47881 cttgtcagtc gatattttaa aaaccaactt tctgtgtgcc gtttttgaat ccattcaatc
    47941 gcttcggcag aattaagacc gtcttctcgt tccggtgtta aaggattatc tcgaatcaaa
    48001 tcgtaatata cttccgttac gtcaaacaaa tcaaaatcga ctcctggaat attttcttca
    48061 ccaaaatccg tagaaaccaa tccgctattc ggtcgttctt ccggaacaga tgagaaacct
    48121 tttttgtgta atgtaatcgt cacttcactt ggtttttgtg cgacttcttc ggctttgact
    48181 agttttccgt tcccgaaaat ccctattccc gcagtaacga gtaacagtac actcattccc
    48241 gtgacaatca tttttttatt catttttttc tccccgttct ttttttatta ataaaacagc
    48301 acctaacaat aaaagaattc ctagtaaaga aatcaacgtt cttgcttcat tcgtttgcgg
    48361 gaatacggac cattttcctg attgacctgg attgcttgga ccactcggct ctttcggttt
    48421 gtccgttgtt tcttttacgg tctcgttata aacacgtggt gttgcggatt cataggcaga
    48481 gactggaact ttcccggatt ctaattcagc catctcctgt ccgttaacta ctacatattg
    48541 tggttggcct tttgcatcca cccaaaattc agggatgatg acctcgattc tcttacttgc
    48601 ttcatccatc tcgtaacctt ccacaccttt taattcttca aaataatagg tgccagaagg
    48661 caagaaacgt tgtcctgtcg taacaaggcc ctctttatcc gaaatgaatt tgcttacatt
    48721 tttgtcattc aaaggatcat tgtctgctgg tttgatccat tgatttttca agtcattagc
    48781 aggagccata tctaaataat atttcccccc ttctatcatc tgatatagag caaaaacagc
    48841 accttctaat ggttttcctt tttcagttgt tcctctttga cgtccatact tgaagaagta
    48901 tggatctctt agataaccga catttttagg atagatatga atctcttgca gttcctcggt
    48961 tgattctgtc gggtgataaa ttggcaaaat agcagcaatt gggatagctg tctgatcgat
    49021 atcaacatcc aatccaactt ctggatcaat cgattcttcg aaaatcaaat aaactgcatc
    49081 ccgatcttgg tttttcttag gtgctttgat acgtgcaatc ccgtctccta atccgccaac
    49141 cgtatctttc ccagttgtta ccgaagtccc tatttggaca agcctttctt ctgtgattag
    49201 ctgacggata ttgtttctgt tcgtttcagc gatttgctgg acgaattctt taggagtcat
    49261 gttttcagca gctttttttt catgataata atcggtcatg tcgtaaatgg caaatgtcgc
    49321 tccatttagt ggcaccgtct gtgaaatgat atctgctgtt tcccctgctt cctcagagat
    49381 agccaagcca tcattttcat ggtattcaaa ttgatcgtta taatcgatat ctcgaatcat
    49441 ccgtttatgc aacacgaatt ccacgacatc ttcccctgat tcttctgccg acatcgcatg
    49501 tggaagaagg actgctgcaa gcggtagtaa aacaagtaat tgaagcatca aacacaacag
    49561 ctttttcatt accttgctcc ctccttttta gattgtctga caaacaaacc ataggctgct
    49621 cctgcaagcg ccatgaagaa tgcagcaagc aggatataga ataatcgtcc ggatcctcct
    49681 gtagcaggca acggattttc aggatcgttc ccgacactaa gctgtaatct gattcgattt
    49741 gtctccaaaa gttcatattc taactcttgc tgtagatcag gcgtctctgc ttcttcgttt
    49801 ggataatacg aaatctcaac ttttagtgta cccgcttctt ttcctggaac tacccgatca
    49861 ttttctatgt caggttcttc tcgataacta atttctaaaa taaagtaacc tggaagtttt
    49921 ctatagctgt caggtgactt cacttccttg atccgataga ccccttccaa caagtctgaa
    49981 gcattgaata gggaagaatc gtttggatct ggtgccaagc ctccctcgat gatttcatac
    50041 tctcctgcac tattttgttt ctcaattgaa aattctgcgc cttcaagacg tcggctattc
    50101 atactgtcaa ttttcaaaag ttccaaatcg attggcgcta gttcatcttc taaggttact
    50161 tgtagtacac catcgatgat ttcatagcct tccggcaagg gagcagtttc gtcttcgatg
    50221 accgtatctt caatgataaa cttaccttct tcattcagtg caaactggaa cacttgatca
    50281 gatagccgat acccatttgg tgcgacagat tccgagacct catattcacc aatagacaga
    50341 tgcagaggtt ggtcgtaatt taaatcgaca tacttttcta ctcctacggc acgagaatag
    50401 aagtatccga tcccaggcaa acgtttcgat tcttctggtt ctaacggatt accagtgtat
    50461 gctcgataca gcgggaagga agaatccccc actttcctga tatcgaaacc tgcaccatct
    50521 aatgtatggc cttcatggtc taattttcta actcggatct cccgataatc aaacgtattg
    50581 taagcagtga atgctaccca taaacgatcc tcttcatctg ttgtatacgt gagatattgc
    50641 caattttctt cacctgtttg cttgagttgg atagtcccta tagcattatc gtaaagtcgc
    50701 aagatccatg tgtcattttc atcaggttct ttgaaacctt ctgggttctg gctctcaatg
    50761 attttgtatt ctgcatagtt ggtagtttct tcactatcct tataaacaaa tgaatctggc
    50821 tctccgattg gatgaataac tttctcacca tccacgatta catatttccc gtcttcatct
    50881 actgaataga acgtgagtct ccctttctct tcctctactt cgttcacacc agaaagccct
    50941 ttcgctactg gatatccgat ttccagtccg ttaccatctg tttcataaag ttcaaactct
    51001 gcgccattga ttggcatatt actgccttca attagtttat tgatattcat gtggattggc
    51061 ttgaattcat ttcgcatttc cagtttgaca tacaactcat catactctgt gccttctatg
    51121 ttaggctctt gtacaacaga aagcttgtga tattctcctt cataatagga attcggatct
    51181 ttaccttccg gtccgccgac tctcgaacca gatgtatatt catctacaag ctcgtagtga
    51241 cctaacatct catcttcgtt ggctgcatat tttttgattt ggaattgata cgtctcatct
    51301 actaactgat aacgtggtgg tccagcgatc tctctgatcc aaaatatgtc gctgactgct
    51361 ccagtttcat ctcctaaaat cggcatcttc gtttcaaaga tcgctattcc gtccgtggta
    51421 gttttacttg tttcgattgg ttcggcttcc ccactctctg gattttggtc atataatgca
    51481 aattcgactc cagccaacgg ctcgccattt tgacccttct tgctgacttg gaaattacgg
    51541 cctttatgtt cgtaacgaat ggaagaagat gggaaataca tccgtccagt agtgatatca
    51601 ttctcataat cctccaggtt ctgaatatag gccatattgt tcactgtgca aaaatttccg
    51661 tcttgagctc ccgcatcaag ttttacttgg tacttgatcc tgaccatttc cccttcacct
    51721 aaagaaatcg gagaaaattt ataagcttgg ttttcccgtc cgacatacgt tgtcggagtc
    51781 gccactactt ctggtggggc ttgtccatga cgttcttcat cccatatttc tggctcttgg
    51841 tacttcatgc cgttttctcc tggatctaga tggtaaaact gaatatctgc cgatctgcca
    51901 gctccaaatg gtgagacatt aaaaccagtt tcatcataaa gtgtggcttg ctggatcgtt
    51961 ctcttgaagg tagaagagat atcctccaag gctgtaacaa tgtcattctt gttgttcgca
    52021 ttgtaatatc gcggtgttac gccatcttct tctaaatcag caatattttt caatacattt
    52081 cgtccaatag catctagtcg ttgtctcccg ctaacgtttc catcgattcc taaaccaatg
    52141 gagaagatac ttggcgggtt ctcttgatac ttgtttttca gccaatgatg ataagcaact
    52201 gtgttgatcg cagatgactt ttcttgcgaa gtaggactgc cattcgtatt gttattagaa
    52261 gaaccatcac caacccagtg agtcgcttta ttatctcctt cttttacctc tccataacga
    52321 tagtgtacat ttacttcatc ttctggacgg ttaaagtcag atgcataggt aactggatga
    52381 gtaaaccctg cattagaagt attcccagaa aactcctcaa aatttctgaa caaaacgtca
    52441 ttatcttcag caatcttatc ttccatcaca gtccagttgc tccaagctcc attagtgctc
    52501 gtacgataac ttgactgaat cggtgcataa ctgaaggtcg gtgtcccatc tcccacaacg
    52561 attaggattt tttctggatt tctattttct ccgccattgt ctttatacaa tgtttcatag
    52621 ccatttttaa gccctagtgt aagcggagtt cctccgattg gtgtacgatt taatgctgta
    52681 ttctcacttc ccgtcaaacg attgatatca tttgtcaatg gaaagatgtc cgaactgatt
    52741 tgctcatggt tattgatagc tgtactatta tggtaaaaat tcaccatacc gattcgaata
    52801 tccagactcg ggtcagagag cagatcttta ctcatttcgt ttactgcctc aatcaacgca
    52861 gcatttttag tttgactatt ggtcccagcg gttaattcac tcatactcgc tgacttatct
    52921 agaacaaaaa cgatatcgat tggactgatt tcctttttct cgcccccgat gacatctaga
    52981 tagacattgt aaagggttgt tgcatcttca ggatgtagat cagatgctgt gctgcttggc
    53041 agaacaggtt ctacccattt tttgatgtac gccccgttat aatccaagta actgttttca
    53101 aaattccgtt catttcccca aagctgaacg gtttgagctg gaacgtttct atcttcttcg
    53161 cctgcagttg atccattatt agtagcatag tcgtaatttc gccagttcgc tcccccgttt
    53221 gtataatcac tatcatcgat caagttctct ccgtcatatg gcgagccttt gtctccttga
    53281 tagcgttcat tgctttgtgt ccaatttcct aacacttttg tatagtcatt cgttgtaaac
    53341 ttcgggtagc gatgctgttc gttttcaact tctccgctag gatagatata ttcatagggg
    53401 tcttgcgtag ctgttggccc aagacgctcc aaaataccgt tgaataactg gcgttgaatg
    53461 cgctggagtg cttgaccgct gggtacgatt ggttcgtcca gcttttccat ctcattttct
    53521 tcgctacgtt gaatttcagc ggaatcttct gtatcagttt cattttcttg taattcttgg
    53581 ttatctattc tttcatcaac caccacttga agaggtagtt acccttcgtt cacatcgggg
    53641 atcttttctg tttcaaaaat caatgtgccg gtttctgctt cttttgaata aacttttccg
    53701 acataccatt cttgaccatc gatcgtctcg acttttaggt cttctccatc ttcatcatag
    53761 gaggatagac ctgttccacg aacgtttttt acggtaccaa tcccggcggc tgccgcatcg
    53821 attgctaaac gtattctacc ttcattggca ctttcctttt tttcaaaagc cagatgccat
    53881 tcaagggttt cttctttttc aatatacttc ccttcaattt ttgcatagtc ctcatctaag
    53941 aatacctcat cctcctttgt ttctgttgac tcagcagcta catccagggt cgtcatatga
    54001 tagactgact ggaaatatgg tccaaatagc aaactgacag ttactaactg caaaagcttc
    54061 aattttaacc ctttcacatc cttatactcc tttcaaaaat atttgtcttc tttcacaaca
    54121 atcaacaaaa caatgcccat ctaattataa tttaatttaa ttttaatttt aaatcaaatg
    54181 atatcctttt tagttaaaaa acaccaatta tacaacattt attattaaaa ttaaattata
    54241 aaattttata acgttctcat ttaacaaaac ttaagttatt aagacaaatg aataaacaac
    54301 tacgctttcc tcgaaacaaa aaaataaagc acaaatgcgc atttacgcac tcatgcttta
    54361 ttcgtacaat taattcagat aacggcagcg cataggatgc caataacact tcctaaaata
    54421 gctcctccta aaatgtcagt aggataatgt aaaagtaaaa aaatcctcga ataagcgatc
    54481 aaacaagcaa ataaaaaggc taatattcct gcaactggac ttaattcgaa caacgcagct
    54541 actgataaaa aggctagcgc tgtatgtcct gaaggaaaag aagaagtctt cggtttactt
    54601 gtgtaaagga caagccgacg atttttttca taaggtcttt tccgtcttgt cgtttttttg
    54661 ataaaaagca aaccaacaaa aaaatagatc aaactgccga taagaatggc tagaccatat
    54721 tctctagtat caggtatcaa gactaaaaca gcagcaagga gcaaccaaat aaaaccacca
    54781 ttagagatat tactgataaa tttcaaaaaa ttgatttttc gactatccgt tttccgttgc
    54841 ttggcgaact tgttgataat actatcatcg attttagcaa tcaaacgcaa catgttattc
    54901 ctccttttta agaacctttt cttgcgtacg atggataaaa agcaggactt gaaaataaaa
    54961 caagaaactg aaacctaata aagcaaaata gattggtcct gcaaaccaga atgcgacaag
    55021 aaggatcaac gtgcctgcca aatagaaaag cgcgcttttg aaatcattaa aaaacaagaa
    55081 aaatgcagta atccaagaag cttctttttg ttccagttga agcaagattt tgtaactaaa
    55141 gagaagcgcg cttaatagaa gcaaaaacaa cgtagtatac atcaatgtat agccaattcc
    55201 cccaccccaa tagcggaaaa tctgataatc gatcaataga agaagaaaat aaacacttgc
    55261 taaactatct tgctttccat aaagagagaa gttctcacga aactttttcc agaatagtga
    55321 accgagcttt ttctcttcat ttttttgtaa tgataaatca tatacagaac tgttcgactt
    55381 gcagttccta atgtgaaaac aggcaagacg cacaggaacc atagtagctg taaatggata
    55441 agacgtggaa tccattcaat gatttctaag aaataatcat tggctttttt accaatcgat
    55501 cgattcatca tccttttgaa cctcctgtca aatccatccc ttcaatgaaa taacgttgtg
    55561 caaaaaagaa cagcaaaatc gtcgggatca agacaagtgt tgctcctgcc atcagataat
    55621 tccattgtgt cgttgcttgg ttctgaaaaa cttgcaagcc aatctgtaac gtgtattttt
    55681 cttgatcttg gatatataac agaggaccta agaaatcatt ccaagctgcg ttgaaggaat
    55741 tgatcgcaat ggtaatcaag gcaggtttcg tcaatggaag catcaaatga ctccaaatat
    55801 acaaatgatt cgctccgtcg atttcagcag cttcgatcaa ttcattattg atcgataagt
    55861 aaaattgacg catcagaaat atattgaacg cattgccgaa gaacgatgga acgatcaggg
    55921 gcaaataagt acctacccag ccgatcttgg agaataatac atattgaggg atcatcgtta
    55981 caaaccccgg gatcatcatc gtagaaagaa ccaaagcaaa catcagcttt ttcccaggaa
    56041 aatcaagctt cgcaaatcca taagcaataa aagaatttga aaggacatta ccgatcacta
    56101 ctagcacagt gatgaataat gtatttcttg catagcgcca aaaaggaaag gcggcaatcg
    56161 tatccagata gttttcccat acgatttttt ctgggaaaaa ggtcggagga taacgaacga
    56221 tttcttccat tggtttcaat gaagtaaaca ccatccaaag caatggaatg atcaaaatga
    56281 ttcctattac tgtcatgagg acataactta gtattttttt gtttgccgat tcacactggt
    56341 ctttttgaaa taatccatca tttgccgtca cctccagaat aatagaccca cttaggtgca
    56401 agcttcaaat tgacaaacgt cagaaccaac acgatcagaa acaggatcat tgacatagcc
    56461 atcgcataac ccatatcaaa agctacgaac gccttgttcc acatatgcag attataaaaa
    56521 agcaaagaat ttgcaggtcc gcctgttcca tttttcgtca tgacataagc ttcttgaaaa
    56581 atttggaaag aaccgatcgt agaggtgatc acatcgtaaa aaataatagg agtgattaat
    56641 ggcaaagtaa tacttttgaa ctttccccaa acgcctgctc catcgatttc tgctgattca
    56701 tataaagatc taggaacatt ttgtaatgta gctaagtaaa gtagcattgc tcctccagaa
    56761 ctccatattt tcatcaaaat caacgcaggt ttcgtccatt gtggatcaaa cagccaagct
    56821 ggtccaggga tatggaacca gcccagaaat gtgttgatca atcctgcaga tggagaaagc
    56881 agctgcatcc ataagatgta gactgctact cctgataaga ctgccggtaa atagaaagct
    56941 gtacggaata cactgattcc tttgattttt tgattgagta ctgtggctaa aaacacactg
    57001 aaaatcgtcg tcagcggtac attgaagatg acgaaataaa cggtgttgta taacgatttc
    57061 caaaataaat catcgtgaac gaacattctt ttgaaatttg ctagaccaat gaaattcatc
    57121 ttggatgtca aattataatc ggtgaaacta ccaataactg aaaaaagcat aggccctagc
    57181 ataaatacca agaacccgat aataaaaggc gagataaata aataggccca tcttgcttct
    57241 tttttctttc ggatagataa acgcttcaaa ttttcttgcc tcctctgtac ctagacagtc
    57301 aaaaatggat tattgatttt gcttgactaa gttttctact gctttttgtg catctgccaa
    57361 tccttctttt ggtgattttg ttcctaaaag aatctggtca atctgttcat tcactaatgt
    57421 cgtgtaatcc ggagcatcta aaggaactgg tgtgatcacc gtatttttaa agttttcgtc
    57481 cgccatttga tagatcattt ttcctttatc cgttaggttg tcattattca ctaaattctc
    57541 attggctgtc ttgctcgcca tgatatcaaa acttttttcg ccgaattttt cttgtacttc
    57601 tggagaagaa agatatttga tgaattcata agaggcctca ggatcttttg ctccgtatgg
    57661 tacttccaag acgaatccgc caccccacga ccaatggcct gaaccttctt ctttttctgg
    57721 gagctgcatc acgccaaaat caaaatcatc cggtgcattt tccatcaaac tagagtaata
    57781 attgatattt tgggcacgca tggctactaa tccggaaata aatggatcag atacaccaga
    57841 cccaaattcg gcttctaatt tattgatcgt atttttccca taataatctt gccagtcttg
    57901 gatccactcc aaggcttcca ctttattttt agtatcaatc ttgacttttt catctttgtc
    57961 aaaccaacta gtcccgttat ctgcgtttag cgcccataca tcagccccta aattccataa
    58021 tggataaaaa ccgatgcgtg taaattcttt tccttctttt ttatctagtt tacgtgctgt
    58081 ttcttctaat tcatcccaag ttgtcggtag atcttgttca ctgattccag cttctttaaa
    58141 tatcttttta ttataaaaaa tgacttgtgt atctgtattg aatgggatcg cataaggatc
    58201 accttcatat tctactgtat cccaaagctg cggataaaaa tccttagaag tgtctttttc
    58261 tacatatttg gagagattag ttgcttgctg tgcttctgct ctttgggcaa cggaatttat
    58321 gtcctgtaca ataacatcgg gtgcatttcc agctgttatt gcagataaag atttagtcca
    58381 gatgtcaccc catggctgat acttataatt tacatggatc tcgttttgtg attgattgaa
    58441 atcctcaatg atttcttcga tagcttcttt tcgtgcaccg gatccccaaa aggaccagaa
    58501 atcaatattt tttcttccat cacttgtttt ttcagaagaa tttgctgcac ttgaactggc
    58561 atcgcttgca ttacttgatc cgcatcctcc taataaaaac aatgctgaac ttgccagcaa
    58621 ccctgtaaac aataattttt tcatatttta cctccaaatt ttaatgtaac cgatgccggt
    58681 tttaatataa cgatatcatt ttgttctgaa aataacaatt tataaggtat gattctaaaa
    58741 aaattattaa tgataccaat actttatata attcgcgagc gctttctttg aattttttaa
    58801 tctttcgtag cagaatgaga atgtattaaa aatagattag gaggaatttt gatgtttaga
    58861 caccagaaag aattgcagtt tgaagcaaaa ccagatcgtc cgaatccatt aatcgcgaaa
    58921 tatctgcagg agctgatcgg cggccaatat ggcgagatga gtgtagctat gcagtacctg
    58981 ttccaaggct ggagctgccg aggagaagaa aaatataaag atatgctgat ggacattgca
    59041 acagaagaaa tcggccacgt ggaaatgttg gcgacaatgg ttgctcgttt attagagggt
    59101 gcgcctgttg aagatcaaga agcagcgatg caaaaagatc caacaatcgg tgccattatc
    59161 tctggaatga atccacagca cgcgatcgta gctggcttag gacctcgtcc tgctgacagc
    59221 gtgggcaatc catggtgcgg tgcctatatc attgcgagtg gcaacttgtt agcagacttt
    59281 agagctaatt taaatgccga atcacaagga agacttcaag tagcacgtat ttacgaaatg
    59341 atcgatgatc gtggcgtaaa agatatgctg tctgttctcc ttgcaagaga tagctatcat
    59401 caaaatctat gggaatgtgc agtgaaagaa ttagaagaaa aagaaggttc aatcttagtc
    59461 ccaagtactt tcccgcgtga caaagaacga aaagacattg cttatgactt ctataacttc
    59521 tcagagggag accaaagtag tgaaggttct tgggctagtg gcaaagcact ggatggcgaa
    59581 ggcgaatatc aatggttgaa agaacctaag atcgaaggcg ctgcaccaaa attagaacca
    59641 gtaacaccaa aaatgtatgg gacacctcca ttcaagaaat aaatatctac ccgtagaaac
    59701 acccgttttt tgttccacac gctttgtgag caaaactgac taaagtcatt aatcataacc
    59761 acccttcaaa agggtggttt tctctagagc tataagctca aatcaccggc cagcgcctaa
    59821 agacgctggc tttcatattt tatgttcaag ccatattgcc tttgccagtt ttgcccacct
    59881 cttaaaaggt ttatgtacta ctccttagcc cttaaaagga tcgtcttact ctcgtacact
    59941 cagcttatct aacgcactat catgtttttc ttgttcttga atgtactttt tgatggtcgc
    60001 ttcattttgt cctacttttg tcacatagta tccttccacc caaaagtgtc gatacccaaa
    60061 tttatatttt agatttgcac aatctcttga tgatctcctg aatattggct cgatattgat
    60121 tataaattat ttttcttcta tactttggtg tgaatacaat gtggtattta cacatctact
    60181 ttgtacgggc taaactattt ccctttattg ctccacccgc atagcaagtg gttttgtatt
    60241 tcaatcgctc ggcgcgctca attaggtcta aagacaataa atctaaaaac ccgctgcttc
    60301 actgacagaa acaacgggtt ttcagaaaaa aatgaaatga aaaagatctc ttacggcaat
    60361 ctattttatt tttttgattt tttataaatg gaaaatgctt caagaagtcc aagaaataaa
    60421 gccgtataaa aacaagaacc tatgaaatct ttatttatta gatattcaac agaaatcccg
    60481 ataacactag ccaacaatat atttatcaga agtgtaaaat aaggatgctt tttatagaat
    60541 agaactagtg ttgttttcat agactctcct ttcttcatat ttccaattaa tcacaaattc
    60601 acggcattac tagaaaaatt aaaggggcgc agaagtggaa aaatactctt cgactttccc
    60661 tacaaaccag gccatcttgt gtttaataac tataccaaat ctaaggtacc tctaaatcta
    60721 aattccattt gtggcggtga cctccaattt ttaactcttc tatctcccat ccagtataca
    60781 taaccttgat acttcgacca cttatatatc gaacacacaa atacctatcc attgcaggag
    60841 tactacctaa cttagttgct acttgcatct aaataacagc tgaaaagata ggacttactc
    60901 ttagtaaagt atctgaacta ttaattttta aactatcatt tttatcctca actacatgca
    60961 catctgttgc aacttcactt tctgtctcat ccagataaat gattaaggaa tcgggaattg
    61021 caatatcttc agctgacgct ggaatagttg agctaaatta gtcatcatat ctttattata
    61081 aaataaccaa cagcaaaaaa tacaatgaaa aacaaacttg gaactaatgt atcattaata
    61141 aatgttgaaa aattcagcaa ctcattactg tatgatttta gcagcccaac caacatatac
    61201 cctataaaag ctaataagat ggttaggaat cctttagaat ttttactcat tcatagacac
    61261 ttccttaaat aacttttatt atagaaggta ttgtaaataa taatattgac aaaaagtgac
    61321 tcaaaataaa aaaaacctta cattatactg gaatgaactg tattgatagt gtacctaaaa
    61381 gctctactga cataagatcg tgtaatcggt aaagtaattt tattaattat acgggatgta
    61441 atcaattaga gaataaacta atcataaaag gtacttttac attcataatt gtcgctttga
    61501 gattctactt tggttcacta tctgacccaa ttgcacattt tcgatcatta caagtattgg
    61561 tgtcgttaga acaactaaat aaaaactagc ggtagatttt tcagaatcta tgctaatttc
    61621 tgtatggatg ttccagtgat ggcaaatttc tcttttcatt gtttttcttg tcgcatgata
    61681 tgcctttttg tgttattcag ttaaactata gaaattcttt cctagacaga tcttgatccc
    61741 aaattttacg tttttgagca tcgatatagt ttaaaatgac attactcata tctaaagagt
    61801 ttagattttt taggactaaa actgtcagtt gttctttttc ttctttctgt taaagtattg
    61861 tcgcgacttt tcgtatacgt taagatatgt cgtgaaaaaa caagttttgt gacacaaaaa
    61921 aacgaatgac ttgaggaaat cacctcaatc attcgtttaa aatgctatat attctttagt
    61981 ttaagatacg gaaattaatg ccatcgacca cactagtaga atgaacgaca tttacttggc
    62041 ctttttttaa tttcgttatg tttagcatat aaaaatatta tagagtttac ttacaatgct
    62101 taaaacatat actgtataat ctgatcaatt ttagccatgt cttgcaatgg taagttttct
    62161 atttttttga gttttcgttc cttaaaatca agagatttta attgtgtaat aattacttgt
    62221 ccttttatat ctagctcttc aggtaatgag taacgtgtcg gtaaattctt tatagtggaa
    62281 gtaattggac atatgaccga aaacattgtt ttacggttaa attcataacg agagacgact
    62341 agtcctggtc gtcttttttg aatttctttt cctacggagg gatcaaaatc aatccataca
    62401 atatcacctt ttttaggaat ataaattctt tcttctgaca ctataaaatt tctctccctt
    62461 caggagttaa atcttcccat tcgtctttct cataatactc ggcctcttcc ccaccactaa
    62521 atggatcttc aatttttgga actaacgtaa ttgttccatc atcagaatac acaacgatat
    62581 attcttggtt ttctgaaggt ttctttccgt tatcagaagg taaagttacc actacagaac
    62641 ttccttgtaa tctagatttt gtaacaagca ttatcatcac tcccttaata aaagtatact
    62701 atataacact aagtaacgca aaattcccct ttgtaaacta tctagaaaat tgttttgttt
    62761 aaagtatatc ttattggata taaatagaaa taaattgtag ccagttcatt ttttctttaa
    62821 ctaaaaaaat aatccaagaa acattgaacc tatggatcca aaagctaccc aaatactata
    62881 gctaaccccc cgctaccttc aattttgcag ataaaacgct ttgggagaaa atttcaaaga
    62941 caacaggcca tcctatcatg cccgattata aacaaaaagc tggctattcc ttttgctctg
    63001 acaaatttca attgggaaag ttcacgaaac tagcaggtga agaagtttca actgttcgga
    63061 tcaaagaatg cccgcttcaa atagaaacat tggttcaacg aatttcaact agacaagact
    63121 ttgctattgt agaacgtgag atcgtagcta tttttgtaga agaagggatt ttacatgatc
    63181 caactcatat caatgtggat aaatggcaac ctcttatcta taaattcaga gaatatacaa
    63241 ctgccaacca gtcgttaact ttgaatttta gattccaaga attcaaagag tcataatgat
    63301 aaacatcgtt aggtataaat aaaaaagact cgcagcacga gtaaaaaaca aatagaagct
    63361 atacattaga aaggtcgact gtgaccctat agatgcagta tatgcagctt atgagcattt
    63421 cttctgttct ctttgttttt gggatctgtg ctgaagtctt tttcgttttt tgccgctctt
    63481 tctttgaaaa atcgattatg ttataccttc tccattacta attcaggcat ctgctgagta
    63541 atgatttttc gcgcaaaaga aatatctgtt acccaaggtt cgatttgttc ttcagtcaag
    63601 acaagcggca ttcggtcatg gattggtgcg actgtgcggt taggttcagt agtcattatg
    63661 attgattctg ccattttttc atcctgatgg ctacgataaa aaccgccaat atacactatt
    63721 ttttccttat ttgaaaacat aaatttgtct ttttcttcat tccactcatt gggaaaacac
    63781 aacgattttg caaaaatggt tgatgaaagg tctttttttc ttctactgtc tctgcacggg
    63841 cattaatcat caattgtcct tttttaaatc cggtaaatcc ccacttcatg atttccggag
    63901 tgaggatgtt ttcttcctct tttcttaacg tcacaacaag atttgacgga aaaacttctc
    63961 cagctgctaa ttctgctgcc tgcttcggtt gtttcgtctg ggcttctcga taataattct
    64021 gaaggagctc tgactctaga tcaaaaaaat aacgaccaca catcctaatc cctcccaatc
    64081 tttttttcta tcatacactg caacaagaag ttcgcaccaa caaaacgctt tcaatttttg
    64141 tagaactaaa aaagttgtga caagtgacaa actctccagt gaatggtatt ctcctgccaa
    64201 ctcctcggtc acaagtcaca atattcaaca aacatgagaa gctgcttttt gaaattgttc
    64261 cttgtgactc ggagtgaaac ggcaggctta cgacctctgg gtaaacggtg ttcaaaagcc
    64321 agtttctcgg tattaagcca aatttatgca aaaatgtaga aagctatttt cacaaaattt
    64381 ttcttaatac tcgaaactaa gcgacttttt cacaacctct tccttaaacg gtgttcaaaa
    64441 gcttgcatct gcggtaataa gcccaaccaa acgaaaaata tgagcagcta tttttatttg
    64501 gctgttctta ttacttgatg cagggcagct ttttcacaac ctctccacta aacggtgttc
    64561 aatcagctaa ccttcggtat tagctcaaaa aacggcaaaa tatgaggaac tattttgccg
    64621 tttgtttctc taatactcag gtcaaagcgc tgatttcaca acctctaata cacggtgttc
    64681 cagaagtagc ttcttcgaaa aataagccga aatttcacaa aaatttgaga agcaattttc
    64741 gtaaattcct tcttatttct tgaagctgac cacttctgtc acaacctcta attattaaag
    64801 aaccttttgc gaagttaaca atctttcttt gatcaattga aaattctgcc taagtagtga
    64861 tagatcctgt gtgactttca acaataagat acttccctgg atttcagcaa tcaacgcttt
    64921 ggcttgacgg tctgcttctt catcggacag tttttcttca gaaagtgcca ggatattttg
    64981 tttcgtatac ttgacccatt gattgactaa ttctagtagc aacagacggt attcttcatc
    65041 cactgtactc aactcaataa tcaaattacc tacaggacaa ccatgataag tgatctgtgt
    65101 ctcatgatca gcgaaaatcc agtccaacat ttcttctaat gcttcttctg gctgttttcc
    65161 agcatttaag atgtcttcga acaattctgt ctgccatacg tccatatgcg catggatcac
    65221 cgctagacaa acttcttttt ttgaagaaaa gtaataataa aattgacctt ttcctgtatc
    65281 agaagctgct agaatatctc ggattgacgt ttcatgatag ccttttttat aaataagatc
    65341 tttggtcgct tccaagatct tttcctttgt tttcaatgat attctccttt ttcatttctc
    65401 atagtcctat attatacagt aaattcatca ttttttcttc ttaatcgttt aaaaaagact
    65461 gtgccaaatt atctgacaca gccctcacta taaaactgtt ctcatatagc tagatgttca
    65521 ttgattcaag aactgcgagg acaaaagcaa ttcctatctt atgatagttg ctctaatact
    65581 tcctctgttg tcatcacttg atctgcaata aagccaaagt tcgtcaacgc tgcatccatt
    65641 gcttcttggc caggagcacc aattgcatct gtcgctacca caacattgaa tccactctct
    65701 accaattcac gcatatggga atcgctacac aagtttccgt tcattccacc aagaattact
    65761 gtgtcgattc cattttttct caattggagc gccaaatcat tgctttctgg tccaaagatt
    65821 ttatgaggag agctgatgat cgtattttca tccaaataag gtttcaattc ttcgataaaa
    65881 tcggctcctg aaccttcttc cactgattca tattgacttt ttctagcaaa catctgctgc
    65941 tcaagcatca tatgttctcc tgcaccgcca aattgccatt tatggtcatg tgggtaaaaa
    66001 taatgaggag aaataaagag tttgtatcct ttttcttttg cttgttttac catttcaatc
    66061 atattttcga tcgtattgta tttttctaag atatcttttg tcaaaccaaa gccaccgcct
    66121 gctggtttca agaactcgac ttgcgggtca gtgagaacta ctgctgtatt tttattattg
    66181 aacatggtgg attctccatt cgttatttat tttaatacat catttagagc ttcactcatt
    66241 gtcggatgag aatagatttg atctctcaat aacgtataag gaagttttcc attcatagct
    66301 agcgaaatca agttgatgac ttcatgagaa tcttccgcat agatacttgc ccctagaatc
    66361 aagtcagttt cagggtcgac taaaactttg aatacacctt taggatcttc taatacttgt
    66421 gctttaggga caccgcttgt catatacttg aataatttgt aattgacgcc ttgtgctttg
    66481 gcttgttttt ctgttaaacc tacattggat aaagtcggag tgataaatac actgtaaggc
    66541 acagctttcc ggttattcgt tgttcgttta ttctcgccct tcaactgatc cataatgatt
    66601 cgataatcat ctagagagat ataagtgaac tgcaatccgc ctttgacgtc tccaatcgcc
    66661 caaatgttgt ctgcatctgt acgaaggtat tcatccacag cgattgctcc gcgatctgtt
    66721 agtttaacat ctgtattttc taatccaagt gatgctgtac ttggttttct tccagttgca
    66781 gccaaaactt ttgacgcttg gattgttact tcttggccat cttctgtaaa ggtgacggca
    66841 gctgttgatt cttggtctgc tacttgatct acagatacac ctagatggaa ttcgattccc
    66901 gcatttgtca tgtcttctaa aatcatttgc gcgatatctt catcttcacg tggaagaaat
    66961 tcagaatggg catcaagaac gacaacttta gatccgtatt cattgaacat tgatgcaaat
    67021 tccaagccaa tatatccagc cccgatgatc actaattttt ccggtaattg ttcctgatcc
    67081 atcgcttgcg tagaatcaat caagtagcga cttgtattca atcctttgat tggtaaaatg
    67141 attggttcag aaccagtatt gatgaaaatc cgttcgcctt caacaatcac tttttcacca
    67201 ttattcgtca cttcgatttg atggttagaa agaaaacgtg ctgtaccgtc catcactgtc
    67261 ccagttgcct catcgctgat catatgataa tttttggcgc gaagcatgcc cgtcaatttt
    67321 tctttccgag caacagcctc tgtaaagtcg attcctcttt ctccattgaa aatcaatgat
    67381 ttagaaggaa tacagccgat gttgatacat gttccgccat acatacgtgt tgacttttca
    67441 acgactaaaa ccgattcacc ttttgaagct aacgccttcg ctaaagtctt accgcccttt
    67501 ccaaaaccga tcacaatatt ttgatatttt tccatcattt ttccctcgct tcttttttac
    67561 ctaccgattg gtacaaaaat atgataaccg ataattgtct attctccaaa tcatctgctt
    67621 tatttgtcaa aaaaaaccag acaaaaagcg gtcctttcac gtttttagtc tggttcgatt
    67681 tcctttattc ttctaaacaa tcgttacttc tacatcgata tttcctctag ttgctttgga
    67741 gtaagggcag aattcatgcg catcttctgc aattttttgg gcttcttctt cgctgatgcc
    67801 atcgatatgc acttctaata ctacaccgat ctgatagccg ccatcatcta aattataaag
    67861 agagacacgt gcttttactg tggactctgc tgtcattcct gcttctttga tcatatgttg
    67921 taccgcccca ttgaaacaag aagcataacc tgctgcaaat aattgttctg gatttgttcc
    67981 ttctgcatgg attcctggag gagtgatttt catatgcatc gagccatttg gtgcctcgac
    68041 ttctccattg cgcccgccat ggttgatcat tgctgtttcg tatgcttttt tcattgtact
    68101 gctcctttcg attgaccatt tgctacttgg ctataattat agtgtatgac aagtaaggcg
    68161 ctttctacaa aaattctgct tattcgaata ccagaatgcc gtcttctact atttttccaa
    68221 aaaacaaaaa aatactactc gtgaacaaca gcgtcatatt tgttccaaaa taaatagcaa
    68281 tcttttctac ataatctttt atatgattga ttttaagata catatatacc agaaagttga
    68341 ccccataaac caatactgtt aaaaaaataa ccatattcgt taccattttt tcctcctttt
    68401 gctggtctcc ctaccagcac tcgatttaac cataccactt tttttgccgt ccaagcaact
    68461 agcctttcaa aaagactggt ttattctgct gttaatgatt caagatcaga aaaatgtccg
    68521 aataattttg ctaattttac taacgccaac ttgttctttc cgaattcatc attttctatc
    68581 tgtaatacta gtaacggctc atgtaagctt aaacggatca aaaaccagcc gtttccgtat
    68641 ggctcttcta gatcatatcg aataccttct tcattttccg ggtcgaagtt catccctttt
    68701 tcttcttcca acagtcgctt catttcttct aatatcttgt ttccttgttc gcgtggttca
    68761 tcgctgaaca attttaatcg gatttcttgt acttctacgg gctgtttcaa ctctttgatt
    68821 tgttcatcta atgtgatacc ttttcttttt aattctggca aaagcatcag gatctttgcg
    68881 atgacatatg caccatcatc cagaaaatag ttttccttga atgcagcgtg tccgcttgtt
    68941 tcgatggcta gctgtgtatc tttcccaaga cggttaagtt ctaatgcttt attgattaca
    69001 tttcgatagc cggaaatata acgaacttgg atgcctcctt tttcttcaat gaatctttgt
    69061 agatgagaag aagtagggga attcgtcacg attcttgttc ctggatgctc ttttaatacg
    69121 attgtactta atactgcgat caaattattt cgattgatca ctgttccaga agcactgaca
    69181 actgcagaac gatccacatc tgtatcgaaa atgatcccaa gatctgcttc atttgctaaa
    69241 acagcctgct tgatactttc catcgcttct ttattatcag gatttgggat atgattagga
    69301 aatcggccat ctggttccaa aaattgggaa cctgttgaat ccgcacctaa gctattcaat
    69361 actttttcag caaagaatcc accagcacca tttccggcat caacaatgat tttccaacca
    69421 tttagtggtt tgctttctag tgttccaatc ccctttttaa tttttgtgac taaatcttgc
    69481 gcataaggtg tcaaaaggtc tgctttttct acgcttccag tttgttcgct ttttattttt
    69541 tgctgctgtg ataggataaa agcaatatct tctttttctg ctcctccttc tgaggtaaag
    69601 aattttattc cattataata atagggaaga tggctagcag tcagcataac cgcagcatcg
    69661 cacgagaact gttcaaattg agtggacata aacatagcag gcgtagtagc taattcgaaa
    69721 tcgatgacat gtatcccttg ttctttcaga ctttggatca acgcctcttt cagtgctggt
    69781 ccgcttatcc tactgtcatg accaatccca atcgtccatt ttttatttgg atcggttgat
    69841 ttcttctttt tctttatcca gtttgctagt ccccagccga tcttttttac catgtctgtc
    69901 gtcaagttta cttcatatcc ttcaactggt aatgcgattc ctcgaatatc cgatccattt
    69961 tgcagcgctt tcatatcttt catataaaag tcctcctcat ttattccctc attttttcca
    70021 gcttttctgc aaatcgctgt gcttcttctt ctgagcggca aataataatg aatgtatcta
    70081 caccggccat cgttccgaca acttcagggt aagccacttc atctagccaa ttcgttacca
    70141 catcggcacc atttcgttca gtcaatacga taaccatgaa ctgaacagac tggatacgca
    70201 aaacctcgcg tctaattgct gtacgcaaac gttcttcatt taaagaaaca gatggctggt
    70261 taaataatgt atagcgtact attttgtttt cattttgact ttttactaac ttcatttctc
    70321 gaatgtctcg agaaatcgtt gcttgagtca catcggcgcc tgcttcatgc agccgcgcaa
    70381 gcagctcttc ctgtgtccca ataggttgtt ctgtaatgat ctggcggatc agtgcttgtc
    70441 gctgtttctt tttcatatta gacacctctc aatgttgatt atagcaaaag ataagtaata
    70501 tttcttcata ttttggtatt ttttactttt attttttgtt ttcattcagg ttgctaacct
    70561 ttttctattt taacacgcaa aatcgtcttc attttttcag gtgctgattt tcttggatcg
    70621 ctgatgtaaa tctctttgtg ttctttagaa gctctttcat agccttcttt tgcgatgaat
    70681 gcttctaatt tctcaaacgt tgctggttct tcggcatagg gcccgacgtg taagatttgg
    70741 gcacaaagac cttcttccat ctttttgaat actacttgtt ctttcaagtc ttccggaagt
    70801 ttgctagaac gctgaatggc ttcttgtgct atcgcttctg taacaaagtc aggctgtcgg
    70861 atcatcaatt gataagaaaa atgttctttt ttcatcactg gttcatgcaa atacttttct
    70921 tgcagtcccc attgtccttc cagtggatag acagtataca tctgataatc aggaatcagc
    70981 caattttttt taggcgacat gcgtattgca tagctgactg catatagaca ggctactctt
    71041 cgtttaaagt cttcctgatt cgggtcgccg atccctctga tgctaaagaa tgatttttta
    71101 ggaacctcta gtatttgcgg gctttttttc atactatagc tcttgtcttt acgccattca
    71161 attttttcca tttttcttcc tccgcgaaat tccatttagt tatttattat catacctctt
    71221 tttgagtgac ttggctaatc gtgttgacac tatttttcag tttgtgctag aataagtgaa
    71281 gtaaataaat cgtacagata acacgtttcg gcgtgtcgag agagaggcgg tttctatttt
    71341 tataatagaa atcactctct ttttttacga aattttttag aggtgatgta aatgacaaaa
    71401 caaaaacagc tgcttttagt acctgcagta attgcaacag ggatgatgtc ttttgcgggc
    71461 gtattgatcg agacggccat gaatgtaact ttcccaactc ttatcagaca gtttgattta
    71521 acgactgcac aagtccaatg ggtaacaacg atttatcttt tgatgatttc gatcattgtt
    71581 ccgctgtcta cttatttgaa tcgcaatttt agtctcaaaa aactcttttt agcttcaaat
    71641 ctgcttttct tggcaggtat aatgatcgat ttcttctctc ctaccttcat ggtcctattg
    71701 tttggtcgat tgctccaagg tgcagcgaca ggtatcgctc ttccgttgat gttccatatt
    71761 atcttgacat ttgcccccct caatcgacga ggcgcgatga tgggtgtcgg tactttgaca
    71821 acggccattg ctccagcaat tggccccact tatggaggca ttatgacttc acattttaca
    71881 tggaatcata tcttcctgtt tcttgtgccc gttttgtttc tttctttgat tatcggctgg
    71941 tttgctattc ccgagatggc agtacaaaaa agtggttatt tggattggat aagcgtttta
    72001 ggaatttgtc ttttgtttag tggaagtcta acatttttga gtacgttggg aacagtttcc
    72061 agctggattg ctttaatcat cggcggtatt ggctggttcc ttttttacta tcgaagtaaa
    72121 aaagtggagc atccactgat ccgactgtct attctttcaa ataaaacatt ccgcttgttt
    72181 ttgattggct tcttagttta tcagttttta ttacttggtg tatcttttgt cttgccgaat
    72241 ttccttcaga tcgttcaagg aagtagtcct tttgtcgctg gattagcgat gcttcccggc
    72301 gcagcggttg gtgctttgct ttctcctttt tccgggaaaa tgctggatca atatggtccg
    72361 aagaagccaa tttttgctgg gctgattctt tctttgattg gttggctagc attattccta
    72421 ctcataggac acgcttcagt gggtgtattg attgcttgtc atgtgtttta tatgatcggt
    72481 atcggattat cttacagcaa tatgatgacc accggaatga atgcactgaa tgaagaacta
    72541 caaggagacg ggaacgcaat gttcaatacc ttgcagcagt tttctggcgc tgtggctact
    72601 tcattagtag cggtcattat caattacgtc cagcatcata cttcccataa ctatgaagtg
    72661 tctactacat taggatcaaa agcagcatta ggcgtcttgt tgctgttgct tctcgtaagc
    72721 tttgctcgat tcgcctatta tctgttcttt gctaaaaaag cgtagattcc atatacggca
    72781 ggtgttgcat ctgccgtttt tttgtgtgaa aatggttgtt tgtaaaagga ggctttttta
    72841 tggaaaaaat tgccattatc ggtggcggga tcattggtat gaccctagcc agttacttag
    72901 atacatcaaa attcaatgtt gctttatttg atgaagggac aggacaggca actaaagcta
    72961 gcgctgggat tatctcccct tggttgtcga aacgcagaaa caaacaatgg tatcgtctgg
    73021 ctaaagacgg cgctgctttt ttccctaaac ttataaaaga tttccaactt gatcaaacga
    73081 tttacgcaca atcagggaca cttttgcttc gacctgaaaa agatttagct gaattggctg
    73141 ttcttgccga gaaacgaaaa gaagaagcgc cagaaattgg aaacatcgaa tggctttcac
    73201 ctgaacagac atctcaagca cttccgttat tgaaaccagc agtttcactg cgtatcagtg
    73261 gtggtggtcg tctggatgga aaagcatatt tagaacatct tggtcagcgt ttagcacaaa
    73321 aacaaatgga gcagatccag gcaaaagtca ctttgcataa gaagagcgat tcttggctca
    73381 tcaagggacc gtttggagaa tacacagctg atcgtgtaat tttgacacct ggcccggcac
    73441 ttaagccttt attacaagaa ttaggctatg ctgcagatat acgtccgcaa aaagggcagc
    73501 tcttagcatt tcagacatct tataagatga gtcaagactg gcctgttgct atgcttgacg
    73561 gagaagctga tttcattcct ttcatggatg gaaagatcct tttaggtgct acccatgaaa
    73621 acgatcaagg ctgggatttg gctcctacta gcgaagctta taagggattg actaagagta
    73681 cagaacagtt cttgactgat ccagatcaga taaaaaactg gccatacgag ttccgtgtag
    73741 gtacccgtgc ttatacgtca gatttttccc catttttcgg cgtactgcca gatgatccaa
    73801 gcttactggc tgctagcggt ctaggctctt ctggcttgac cacaggtcct tatatcggct
    73861 atctattagc caacttctta aatactggtc attggcctac ggaagattat caaaaggaca
    73921 tctccaccta tattacgaaa aatccatcat agaaaaacag ctttttcaag taaggatccc
    73981 gagcgatatt gcgcatggat tacttcttga aaaagctgtt attttagttc aatttctgaa
    74041 tcaaattctc caaactttct tttgcttcat ctggcaagag atcttttcct atctgagctt
    74101 caatgaatcg taaacgaata tcaagtcgtt gtttcaccag atttttatag gacgtatcct
    74161 caaaagaaac aggaaaatct ggtataggta aatagtggat gtcttcaaat ggaccaaacg
    74221 acttgaattc tgccgtttgt tgcacgattc tttcaacgat tgacaatgtc gtttcaggtt
    74281 tgattttttt atcatcgaaa tatccagtat tcaggacata gcaggctgtt tgctgtttca
    74341 aaaacaactc tcgaaaagct tgatagtctt ccctaagagg atagacacgg aaaggattag
    74401 caaatggttc aatgactagc tcatctagct gtctttttgt agtgatattt tccgcgtctg
    74461 atcgttttgt tgctaatgtt gcaccaaaag acgctgctaa aactgggtca ttgatcttga
    74521 taattggcgg aagcgaatca tctttcatga tccaaaaaac agcatcgatc ttttcgctaa
    74581 gatgatcgac tcggtcgggt gtcgcataac gtgatttgac tgtccgccca tttccgtttc
    74641 gtatatctcc tgttactagg actttttctc ctttacttgt acatgtcacg cccacattct
    74701 gacaagtaag gaaagaagaa atggccggat catcagacgg atagtcttgt gttttatcaa
    74761 agtaagaagg ttccaaggca gtagcaaaac cattttttcg agaaataacg aaagcatcgt
    74821 catgcaagat cgaaacagag tgaccttccg tatgttttgc taaggtaatc gttgattttc
    74881 cagaaccgga caacccaaat acagccatcg tataggtctg gtcattacta gcatattgtt
    74941 tcatcccacc gtggcaggca ataaagccat tgcgatttcc caatgcccaa gcaagcgtca
    75001 aagtcgcttt ttttaattct ccaaagtaac gtaatcctag cacagccgct gtattatgga
    75061 gagcatcaaa caaaatgagt ccatttggaa atgtttcatg tgtccattcg ggatcacaaa
    75121 agatataaag atctccctca tcaaaggaaa cagacttttg ataagtctct aagatcttcg
    75181 aattcgctgg ctgaaaattc agcaaataag aatacaaatt gttctcaaat ccttctggaa
    75241 tcagcaaatg actttgcaca gaaaattctt cagataggcc aacgattact tttgtatggt
    75301 aaaactggcg cttgcgactt tgatacacag cttctcttaa aattttctcg tatttgcttt
    75361 gttccttgtc taagtcctca aagatccgcc gagcacttgc tgttcttccc acaatgcttc
    75421 catcattgaa tactagtgtc ctaacatcag ctggtaaatc tagttcttca gcatgtgaga
    75481 ttgttaaatc tgtgatgatc gttcctggac tcccttttgc tagttgataa gctgtcttcc
    75541 tatcaaaaac ttcatgaaca ttattaccgt aaaatgtcgt ttctacaatc gatcgaatac
    75601 tggataacag tggatttcca cttgtgattt cgtttaattc gagactctct tttgtactca
    75661 tggctcctcc tcattcccta aacaaaaaaa cgttttcttt attatacatt attcgagttt
    75721 ttatttgtac tgctgcgctg tgggaagcaa acgagttatt tttcacacaa aaaaaccgag
    75781 aacttgaata aacaagtttc tcggtttcat cattttcctt ttatcggctt agtaccagcc
    75841 gtttgctaac cagaatgttt tagcagcatc ccatgaacca taacgagatg ttacatattg
    75901 ttctgctaca cgttcttgat tggcagctga atagtcgccg tttaagtatg aagcatctaa
    75961 ttgataacgg ccgatgtaac gtccgttagt agctgtgtat gaaccgcctg attctttttg
    76021 tgcgatccat tcttttgcag agcttgaagt tggcgctgtt gcagcagctg gggcagcagg
    76081 agctacttca actgtttcaa caggtgcaac gtatgtatgt tcttgcgttg ctgtttcagt
    76141 tgtagatgct gtattttctg ttgttttgtt ttctgttgtt gctgaagaat catttgaagt
    76201 tgggattgtt aattgttcac caacataaat gcggttgatg tttgcgatcg aatttttttc
    76261 agcaattgca tcgatcattg agttgtctcc tgcaaatttt tgtgagatct tagataatga
    76321 atcacctgat tttactgtat aaacttcatc agcgtgagca tttgtagttc ctacaaacat
    76381 tgcagtagca ccagcagcaa aagttgtacc taaaacaatt ttcttaaatg aattcatatt
    76441 aaactccttt ttttctccgt tttttattct ttcgacgcta ttaactttat catttagaaa
    76501 tatttcatag gtaaaagaaa catgacattt aatgacaaaa taacgaatta tttaatattt
    76561 gtaacattta aacaaatatt tttttgatac aaaaccataa aagccttgtt gtataagcaa
    76621 agtaatcgtt tttctatgtt tttttatttt tgaatgaaaa acgcagattc tgtaacattc
    76681 actgcaataa tccataaaaa aaagcaaaaa aatttttgat ttttatcaaa ttctcttaaa
    76741 aaaatgtaaa aaatacaaaa aaaccagtaa ttgttacaac aattactgga caaaaacttt
    76801 gaataatgtt aactattttt ttattttaac tttctccgca ataatttctg ggcatgttcc
    76861 aattcgttta taacgacttc ttctgtttct ttagctaata tttcatctaa tacatcaagc
    76921 catttttccg gttcttttgt tcctagacgt ccaatagccc aagctgctgt tcctctgatg
    76981 acagggcgga catctgttaa acattctgaa atctcaggca atgccgttcg atcacctaga
    77041 ttagctaaag caatcaatgc gtttctctgc aaaggtttct tcccacgcca tgaaccagct
    77101 aaatgcccaa attgctgttt gaagtcttta ttagacatcg aaagcattgg ctttaatttt
    77161 ggatatattt cgtctacttt tggttccatc tcttcatgaa aatgaaaatc ttttccttga
    77221 ttgtaagggc agactaactg gcagatatca catccataaa tgacatttcg cattttttta
    77281 cgatattctt ccggcatcat cccttttgtc tgagtttgat aagacaaaca tcgctgggca
    77341 ttcattcttc cgtcaccaag caatgctttt gtcggacaag cagtcacgca gcgcatacag
    77401 tcaccgcaac caaattctcc tggtttatca ggctcaaata caatattagt aacgatctca
    77461 ccaagataga caaatgaacc gaactcttca gtgatcaaca atccgtttcg tccaataaat
    77521 cctaaaccag ctcgctgtgc tacagcgaca tcgatcagtt ctccagtatc tacttgcgga
    77581 gctagacgcc attcctcttc tttttgccat ttttctgctc ttgtttggat gaattcgatc
    77641 aatcggttca aacggtctct gagtatatca tggtaatcga tcccccaaga agcacgagca
    77701 aactgcccgc gtttttcatc cctaggcact tcttcatgga tcctagtagg atacgcaagt
    77761 gcaatggaaa tgatcgattt cggctgatca aatgtcattt ctggataaag acgctcatcg
    77821 atatttggat gttcaaagcc agaagtatgg ccagctgctt tttgttcttc caacgattct
    77881 tttaatgaat caaaaggttc cgccgtggtg aacccgattt tgtcaattcc taatcgtttg
    77941 ctttcttgta tgatttcttc ttttaatgtc atggtcttcc tcgcttgctg ttaacggctg
    78001 tataacccat aatatagttt ttcttttttc agctggacaa actggatctg ctgatcggca
    78061 atcttctttt cttccatcca tttttttctt gcagcaaata cttcccaata atttgttgaa
    78121 attaattttc ttttgttatc taatgcatta aaaaaattca atgaaactgg ttttgtactt
    78181 aacagttcat ggagccttac ctcttcccat ggcgaaaact cagacagatc gcgggaatac
    78241 aatacttctt catagagatt tttttgctgt gtgtacgtga atgtttgcgg gattttccca
    78301 ggcctagcaa tttctactag acgattataa aacatattca ttaatttgct gttggcaata
    78361 aattgacttt tataataggc cgactggatc tttttgatta ctccttctgc tttgatgact
    78421 gaaaataatg caggccatgc tttgacggtt atttcgtcgt tttgtttaac taatacgcca
    78481 ggtgtgaagc cgactggaga aaggaacaca aaataaccga taccggtatt ttcatctaaa
    78541 ggctgaaatt catcaaacaa ccatttgcct atgtacagct cttcatctgg ttctgttgtc
    78601 tcattttgat ttgctatgaa taatttagct aattctgctt caagaaattg aaggtcttga
    78661 tccttgtcgg tattttcctg ctggctgatc aatcgaaaga cttcttcttt aatcgtatat
    78721 ttatctttac gtgtaaaatt catagccaca ccttccttcc ttactctaac tataacggaa
    78781 tttcttcaaa aaaggtatct gtacgacgaa aaacagcata attttttttc atatctctta
    78841 taaaaaatgc aaggtctgct cctcaagcta gaccttgcac ttttcagctt tcttttgttt
    78901 gtgacaaacc ttcatggatc gtttcaatat tccatttcat catctgatag taagaatctc
    78961 cttgttctcc ttcttttgct atggaatcag tgaacagttt atcatagatt ttcaaacccg
    79021 tttcttttgc tacccgttcc atgcttcttg agtctacact ggtttccacg aataaaaccg
    79081 gtacttcttt tgctcttatc tgatcaatga tcgctttcat ttgatcaggc gtaccttgac
    79141 tttctgtatt gatctcccat atataagctg ctggcaaatc atatgctcgt gaaaaatact
    79201 tgaatgcccc ttcacttgtt actaataatt ttttgttctc ttcgatagaa gcaaaacttt
    79261 ccttcgcttg tgtatctaat tcttttagtt tttccacata tgcttctgca ttttttttat
    79321 agagtgtcgc attttctgca tctttttcag agaatatacg tgcgatttcc tccacatatt
    79381 ttattccgtt agataggtct agccaagcat ggggatctgc ttttgtatgt tcttcaccgc
    79441 tagttaaata aagaggttct acatttttgc taactgcaaa ataatctttt ccttcttctt
    79501 ttttagccgt ttccatcaag ttatcgaacc agctgttacc tgtttcaaga ttcaaaccgt
    79561 tgtataaaat aacatctgca tcgcttgcct ttttgatgtc ttctggtagt acttcatatt
    79621 catgcggatc tgttccgact ggtacgatac tgtggatatc tatttgatct gttccgacct
    79681 cttttgccat gtccgccaag atcgaattgg tagccacgac tgacaacttt tctttacttc
    79741 cttcatccgc ttggtcagaa gcctgctttc cgcaaccggc taataccaag aaactaactg
    79801 ccaagattaa aaagagtgat ttttttgttt tcatctatct gcttcctttc tgaaataaaa
    79861 ttccttttgt tggagaaaat aaaaaagcaa ggataaaaaa tgcggtacat gctaacacga
    79921 tcgtagctcc tgatggccaa ttgtaactat aactgaaaaa cacaccgaca actgcactta
    79981 gcataccgat tcctgcagct gtaatgatca ttttcagtaa atggttcgtt agcaagtaag
    80041 ccgtggcggc tggtgtgatc aacatggcaa tcaccaagat tgttcctact gtctgtaaac
    80101 tgactacagc cactaatgtc aaaaagaaca tcaaaagata atggatcagc gatgtgttca
    80161 gcccataagc ttttgccatt gtcggatcga atgatgtgat cttcaattct ttataaaaga
    80221 aaaagacaaa tatcaagacg attccactaa cgagtgctgt cagcaacaaa tcgctttccc
    80281 taacagctaa gacattccca aataaaatat gatacaaatc tgtcgcactc tgggcatatg
    80341 aaatcaagat aatccctaat gcaaaaaagg agctaaagac aattccaatc gctgtatcat
    80401 ttttcaatcg gctccgctga gtcacgaaac caatcactgc tgctgttagc atcccaaaag
    80461 cagtggctcc aaagatgtaa ctaaaaccaa acatataaga aatggctaca cctggaagaa
    80521 ctgcgtgaga gatagcgtct cccatcaaag acatccctcg taaaataata aaagaaccga
    80581 tgactccaga agcaagtcct acaagcatgg aagtgaccaa agcgtattgc aggaattgat
    80641 aattcattaa tccatcaata aaggattgga tcattttttc tctcctcctt ttactaaatg
    80701 tctgatgatc tcaccatagg ccaattgaag gttcttcgat gtaaagactt cttgtactgg
    80761 accggaagcg atcagctgtt tatttaaaag aataacctca tcgaaatatt cttctacttt
    80821 atgaagatca tgatggacga tcaaaatcgt ttttcctgaa ttcttcaatt cctgcaagat
    80881 gtcaaagatc tttcgttcac ttaacgcatc aatccctacg aatggttcat ctaaaaagat
    80941 ccattctgct ccttgggcta gagctcttgc aataaaaact ctctgaagct gtccacccga
    81001 tagttcgctg atctgtcttt tggcatattc ttccatccct acttttttca aagcttgtct
    81061 tgcacgttct ttttcttttt tcccaggtct ttgtccaatt cgtaaagatg gatatgttcc
    81121 taaaagtaca acgcctaata catcaattgg aaaggaaaga tccaattcac ttcgctgttc
    81181 cacgtaggct atttttttac ggactgagtt taccgactgt ccagaaaagg tgactttacc
    81241 agtagtagct ggtatcaact ctagcaaaga tttcataaat gttgattttc ctgcaccgtt
    81301 tggtccgata ataccggtaa ttttttgtgg atttatcgtc aagttgatag ttttcaaaac
    81361 agttttccct tgataagcgg ctgaaaggtc ttgaattaca atggccgtac gttccaaatc
    81421 ttacactcct ttttaggttt acctaacttt ttatttaaat atagtttaac taaaatggaa
    81481 agcattttca agtagaatga tgaaaaagtt tttttcgaat tttcctcgca ttttatgaaa
    81541 attttctgta gaatcacatc aagagttcaa taaggctaac ctaataatga aaaacatgtg
    81601 ttgaaacgtt tgattatttt ttcttgttga ctcaacaacg aaaggagttt ttgtatggaa
    81661 gaagaaaagt taactcgaag tcttagtgcc cgtcatatcc aaatgatcgc actaggtgga
    81721 acgatcggtg tcggtctgtt tatgggtgcc tcctcaacca tacgttggac agggccctca
    81781 gtgatgggag cctatgcgat tgcaggattc tttttgtatt tgattatgcg tgctttaggc
    81841 gaaatgcttt acgttgatcc ttcgactggt tcttttgcaa attacgcgag cgagtacatc
    81901 catcctgtgg ctggctactt gaccgcttgg agtaatattt ttcaatatat tgtcgtagga
    81961 ataagtgaag tgattgctgt aggatcttat atgaattatt ggtggcctga tttgccggca
    82021 attattcccg gaatcattgt cgtcttgttt cttatgctgg caaatcttat ttctgtcaag
    82081 gcgtttggcg aattagaatt ttggttttcg atcatcaaag tcataacgat cattttgatg
    82141 atcattgcag gtcttggggt aatcttattt ggatttggta atcatggtca ggcagtaggg
    82201 atttcaaatc tttggaaaaa tggtggattc ttcactggag gcgtcaaagg atttttcttt
    82261 gcattatcga tcgttgttgc ttcctatcaa ggaatcgaac tgatcggaat gactgctggt
    82321 gaagcagaga atcctaaaaa gacgatcatt gaagctgtcc agtccactat cggtcgaatc
    82381 ttgatttttt atattggtgc tatttttatc attgtgagta tctatccgtg gaatcaattg
    82441 agtgagattg gttcaccttt tgtacaaact ttttctaaag tcggcattac atttgctgca
    82501 ggactgatta attttgtcgt gattacagcg gcattatcag gttgcaattc agggattttc
    82561 agcgcaagtc gtatggtcta tacacttgcc ataaatggaa agatgtctaa aaaatttctg
    82621 aaactatctc gtcacggtgc gcctttttat cctgtcgtag caatctcagg tggaatctta
    82681 attggtctga tcttaaatta tcttctacca atgttcatca accagtcaga aaatattttc
    82741 gttttcgtat acagctcaag tattttgcct ggtatggttc cttgggtagt gattttgatc
    82801 agcgagattc gcttcaaaaa gaagcacgca gataaaatgc atgatcatcc ttttaaaatg
    82861 ccactatctc cttatacgaa ttatgtgaca cttgcttttt tagctatcgt attgattttc
    82921 atgtttatca atcctgaaac gagaatctca ttgattattg gactcgtttt cttgctttac
    82981 atgacgatcc attactttgt acgcgaaaaa agaaccggaa aataaaaaaa gaacgaagct
    83041 tcaccttagt ggaagcttcg ttctttttta ttgcttttat caattcaatt ttagcgtctc
    83101 accgggagtc aggaaataat tctccatgct catcccattc agcgacaaga tcgtttctac
    83161 tggtacgcca gttcgttcgg ctatttgatt tggaccttct ccttccaaaa ctacggttgt
    83221 cgcagcctgt gaagtcgcgc tgttggtatt agtttcagag gaatctgttg tgtcttgtgt
    83281 tgttcgagct tgcatttcag aagcttctac cgactctgct gtctcagaat cagaagatgt
    83341 tgtgtcttgt gtgcttgttg actctgcttg aagagaggcg ctagtctgca aataaatatc
    83401 ggaaatctta gcgacatgat tcgtataatt agccgataat tgattgatca aagtatctaa
    83461 gtctacaccg tctttatttt ctgctgcctg atacgctgca cgtaattttc tgatcccttc
    83521 gtcggagatt gcttgaagct cacgtgcttt ttggttagca actgctgata aaccagctat
    83581 tccctcttga tttccctgaa tttctgtctg gtattcttca atcaatgcag gcgtcttttc
    83641 ttcgatcttt ttcgaataat catttacgat tgcttgaagt tgctctggca aaccatttgc
    83701 tgttttttct tcagaagttt gtgtctcttt tgttttagtt gatgactcac ttgtttgttt
    83761 cgtctctgtt ttctttgtcg ttgtttccgt tatggtcgta cttttcgctt ctgacgaatt
    83821 ttttccatga aaatatagac ccgcaactcc tcctgctatg atcaaaaaca cggcaactgc
    83881 tgccccaatc atgatcatcc gcttacgagc tgcctgtttt tgctgtcttc ttcgggtcat
    83941 ccgaacattt tctccccact cattttcatc atacattttt ttcaccacca atttttatgt
    84001 ataatagcgt tatcaaacgc ttcatattct tattgtagca aataattgtc gctgtgcaca
    84061 tttttatctt tgttatttat aagaaaattt caccagttga gatgattcca agattgtccg
    84121 tcgatttctc cttgcagctg cattgttcgt tcgtctatct cagccatcag tttctttatt
    84181 tcatataaga gcgcacggtc ttgatattct gatttttcgc ttaattcatc taattgttgg
    84241 atcagccaat catttgtcat aatgaagttc ctttcatttc ttgcagtgta aaggtcaagt
    84301 cttcgatctg ctcttttgcc tcagtcagtg cttctacgat atatgcttgt ttttcagatt
    84361 cttcttcctg cattttctga ttttcctgta ctctttgaat aaaccagtca atccgctgag
    84421 tcagcgatgg tttgccttcc aatgagagat actgctgata agcaattagc ttctcccaac
    84481 gtttttcctg ggataaatac ataaattgtg ctaaaacgaa tggcggaaga taggtcatat
    84541 ggaatttatt tgcgattcgc tgatacggcc tcaaaaattc ggaaatcgta tattcttctt
    84601 ttcctccagc ttggtattct ttttccgcca cacctgttgt caatacgatt cctagttctt
    84661 tcccttttaa tacatgtgct gctcgttcca agacattatc ttgccaaatt tttaaatgag
    84721 caggagaact ataccagtaa aacggaaact ggaaaatgat ccgatcatgc tgggccaaga
    84781 gctgtagttc agtctctata tctatttttc cgtctggata acttgattcc aaatgatgcc
    84841 aagtagctgg taaaccagca gaagcttctt tgaaaaattg ctgatttaaa gattcttcga
    84901 tcgttggatg gctaatgatg ataagtgtac gcatcccttt tcctcctttt tagtcttcgc
    84961 tttacagtgt agcataaccg ccacaagttt cttttgccga tgcttgtcat tttaaccaga
    85021 ctaatcaggt tgtccaagaa actgcggttt gtttttccaa ctgttttttc aaggtatcca
    85081 aagcaatatt cttaaaattt tcgtttttca tacgtttacg atagttcgta gccgtttctc
    85141 cggtactctt tttgaacatt cggctaaata cgactagact attgaaccct acttgatagg
    85201 caatactttc cacggattca tccgtcagag aaagaagaaa cgcccctcga ttgatccgta
    85261 ccccgatcaa atattccttg atactggtac ccatatgatt tttgaatact cgcgacaaat
    85321 ggctccgatt gaccgctaaa tgctgggcga tttcctcaac agacaatttt tcagcataat
    85381 gtgcctcgat gtatttgact gtttcaacag ctaaagagga atgaaccttt tgtacaccac
    85441 cgctgacctt tccgccatct tttaaaagga ctgataaaaa acggtaggtt aatgctgtta
    85501 actgtaattc attttccaag gtttcttccg tatctttcat gcattctaaa ataatgtcga
    85561 tatatttttg tatgtcagaa gagaccatcg tgtattggtc ttctctgaaa agcgactgcc
    85621 gaatgatttc ttctgcagca ctcccaccaa atgaaagcca gcaatacatc cacggatctt
    85681 ctccatctga taagtaaaac gtcgagtctc ctggacgaat caagaacaga tcaccttttt
    85741 tcaattggaa ttgttgattt ttcacatgat aagatccttt gccttccatc acgacatgca
    85801 ggatgtaatc attgcgaata gcaggaccaa aagaatgaaa cggcatcgtt ttagagaaac
    85861 cacaaaaatc aaaatacaat tctttttcta cttgttgatg ttcctttttt aaaaaaatcg
    85921 attgctccac gtctatccct ccttactcct ttagtataat gcaacaaaaa gttaaataaa
    85981 agtcacagaa atatattaaa aaggaagcga tttcttgtta agtttaagtc attaaaagat
    86041 ctttgttatt cgttgtaacc ggtttactta gtattatata ttgaacagga gaggaataca
    86101 gattatgaaa agcaaaatga tccaacgtgt gtcctacgct ttaggcgctt ttggacacga
    86161 cacattctat gttacattaa gcacttattt tatggtattc gtgacaagcc agatgttcgc
    86221 aggaacagat aaggcaacga atgcaaaaat gattgctaca gttacaagct tagttgtagg
    86281 tatccgctta gttgaaatta tttttgatcc tttgattggt ggcatcatcg ataatactcg
    86341 tacaaaatac ggtaaattca aaccatggtt agttatcggc ggcgttgtta gttccgtact
    86401 cttggtagca atctttacca atttctttgg cttagctact cataatacga ctttatttat
    86461 catattattt gcaatcgttt tcatcttatt ggatagtttt tattcattta aggatatcgc
    86521 tttttggtca atgattccag ctttgagtac ggaaacaaag gaacgtgaaa aatttgctac
    86581 gtttgctcgt ttcggctctt ctctaggtgc aaatggtact actctagtcg ttgtccctat
    86641 cgttactttc tttacctatc ttgttactca taaacatatt gaaggcgctt caggctggtt
    86701 ctggttcggt gtgattgctg ctgttatttc aggcggtact gcgattatca ctgccttggg
    86761 cacaaaagaa gtcgatactg cgatccgtca gcaaaatgaa aaagctggga tcaaagatgt
    86821 gttcaaagca gttgctcaaa atgatcagtt gatgtggctt gcccttgctt atatcgctta
    86881 tgcgattgcc aatgcagcaa caactgccgt attattctac ttcttcaaat atgtacttgg
    86941 ccaaccaact gaattttgga tcgttggtct agttgctgcc ttacttggtt tagtagctgt
    87001 tccactattc ccaatgctta cacgcgtcat ctctcgtcgc tacgtttacc ttggagggat
    87061 cgcaatgatg atttcttctt actttttctt tacgatcgca ggttcaagtt tgccaatcgt
    87121 tatcatcggg ctagtattgt tctacttccc tcaacaattg attttcttat ccgctttgat
    87181 gacgatcaca gactctgttg aatatggtca atggaaaaat ggtatccgta acgaagctgt
    87241 tactctatca attcgccctt tactagataa aatcgctggt gctttgtcta acgggatcgt
    87301 agggtttgtc gctatcgctt ctgggatgac tggtaatgct actgctgctg atatcactgc
    87361 tcatggcgta acgactttca aagcttatgc attctatgct cctgctgctt tgatgatttt
    87421 ctccgctatt atttttgctt ggaagattac attgacagag aaaaaacatg cacaaatcgt
    87481 tgagaaattg gaaaacaaat tggctccagc tgatacaatg gaagatgaac ttcacgatgc
    87541 agtagatcct tcccattaag aaaggatctt gaaaatgaag attacagaaa aagattttgg
    87601 ccaaggttat cacttgataa ccttggagaa caaaaacaag ctagcacttt ctatctctga
    87661 tttaggtgct agaatcgtca gtttaaaatc aaatgatcga gaattagttt tgggatttga
    87721 caccgctgaa gaatatatcg aaaaagatcc gtatatcggt gcttctatcg gtcgcaccgc
    87781 agggagaatc gaaaatggcc gcttttctct caatggaaaa acttaccaat tagccacaga
    87841 tccaaaaact ggacataatc tgcatggtgg tgctcctgga tttgaattaa aaaaatggtc
    87901 ttatgtcatt ttgaatggcg aaaatgaagc ttctgtcatt tttactacta caagtcccga
    87961 cggagaacat ggttttccag gtacaatgga tgtggaaatc cgctatactt tgacaaaaga
    88021 taatatctgg cgagtgacct ctcgcggcac cagtgaccaa gatactttat tcaatccgac
    88081 aaatcatgtt tacttcaact tgacgggaga tgcttctcag tcaatcgatc aacatgagct
    88141 ttggctcaat agcgaagctt atgctccttt gcgaacagat agtattccta taggggtaaa
    88201 agaaaatgct gcaggaagtg ctttcgattt tcaaatacct aaaaaattag cgtccgtttt
    88261 tgctagtgat ttggatcaaa aaaatcttgt tgacggaatc gatcatccat ttttcttaaa
    88321 agaaaccggc ttgggtaaag aagccgctcg cttgactagc cccgataaaa gaatccaagt
    88381 agatattgct acagatgctt ctagtgtggt tattttcact gcaaatttcg gcacagaaac
    88441 accagaaatg cgcaaccgaa aactggcaca tcacggtggc atcacatttg agactcaaac
    88501 agctccaggt gcagagcgct tctcagcatt tggttcgatt catttaaaag ccggatcagt
    88561 atttgaaaca gtaacagaat tcaaaatcaa aacaagaaag gaataactca tcatggaaat
    88621 caaaagaagc ttaaacacaa aatttgccga aacattcgga cctaaaaaaa ctacacaata
    88681 cttctcacca ggacgcatca atttgattgg tgaacacaca gattacaatg gaggccatgt
    88741 cttccccgct tccatcactt atggaacgca aggtgtagca gcacctcgag acgataataa
    88801 aatattagtt tattccacaa actttgacga tatcggtgtc attagtttta ctttagatga
    88861 gctagaatat gataaaaaag caaactgggc aaattacgta aaaggaatgg tccttaaatt
    88921 aaaagaagct ggacactcaa tcgatcatgg gtttgagtta ttagtggaag gaacaatccc
    88981 taatggtgca ggactttcca gtagcgcttc attagaatta ttagtaggtg ttgtattaga
    89041 agatctgttt gatttaaaaa cagatcgctt ggaactagtc caaacaggta aaaaagtaga
    89101 aaatgaattc atcggtgtca attcaggtat catggatcag tttgccatcg gcttcggtga
    89161 gctagatcat gcaatcttat tagatacaaa tacgctgaaa tacgaaatgg ttccagtgaa
    89221 actagatggt tatgcaatcg tcatcatgaa tacgaataag cggcgggaat tggcagattc
    89281 aaaatacaat gaacgccgta gcgaatgcga agaagccttg gcacgtttgc aaaaaacttt
    89341 gcctatctct tctttaggag acttagatga agaagaattt gaatcaacta ttgaccaaat
    89401 cggcgatgat acattgatac gcagagctcg ccatgccgtt tctgaaaatc agcggacttt
    89461 gaaggcaaaa gctgaattag aagctgggaa cttagaagca tttggtcaac tgttaaatga
    89521 ttctcaccac tctcttcgct atgattatga agtaactggg atcgaattag atacactagt
    89581 agatgctgct caaaaacaag aaggtgtctt aggtgcacga atgacaggag ctggatttgg
    89641 tggctgtgcg attgctcttg tcaaagaaga aaatatacct gccttcaaaa acaatgtcta
    89701 tgatgaatac atgaaagttg tcggctatgc tccggaattt tatgttgcac acattggaaa
    89761 tgggacaaca aagatcgatc cagaagctgt tcatgtttga taaaaaaatc aacgatctca
    89821 gctaaaaaca aatagataaa aataacctct cagcaatttc caaatgtgag ccttttgctc
    89881 gtatttgaaa attgctgttt tttattgaca cggaaagaga acgtgtgttc taattgtgtt
    89941 gaagaggtga ttgttatgtg ggcgaatcaa aatccggtat ttgtttatga aaaagaacct
    90001 tcaagagata ttttatgtat tgactgcaaa tctttttacg cctccgtaga gtgcgttgaa
    90061 aaggatcttg atccccttac aactaaatta gtagtcatgt cctatccgtc cgattcgaca
    90121 gaaacacggg gtagcggatt aatcttggct tccagtcctg cagcaaaaaa agcttacggc
    90181 atcaccaata tcagccgtgc cagagatctt cccttcccct atccaagtga tttatatatt
    90241 gttgccccaa ggatggccta ttatatggaa aagaacagag aaatcaattc tatctataaa
    90301 aaatatgcag acgaagcgaa ccatcatgtc tattccgtcg atgaaagctt ccttgatgta
    90361 acaaatagca tggcactctt tggtaaaaca aatgcgtacg acatggctaa actgatccat
    90421 aaagatgtgt ttgagcagac cggaatctac acgactatag gaattggcga taacccgcta
    90481 cttgctaaac tagccttaga caatggctca aaagacggaa agcaaatgtt tgctgaatgg
    90541 cgttatgaag atgtaccaga aaagttatgg aaaatctcag atatcactga cttttgggga
    90601 atcggcaaac gaacagcaaa acgtctgaaa caaatgggta tacattctgt ttatgattta
    90661 gctcacgcgg attattatca cttgaaaagc aagctagggg tcatcggtac tcagctttat
    90721 gcccatgctt ggggcatcga tcgaagtttt ttgggacaag aatatacacc aaaatcaaaa
    90781 agtatcggaa atagtcaaat cctagataga gattatacaa gacgagaaga aatcgagatc
    90841 gttatcaaag aaatggccga ccaagtcggc actcgcttga gacgatctgc agcaaaaaca
    90901 caagtagtca gcttatgggt aggatactct ttaggctatc ttgatccaaa tggcaagacc
    90961 gggtttcacc agcaaatgaa aatcgaagcg acaaatgcta gcagagatct agcagatgct
    91021 ttattgacca ttttccacca gcattaccac taccaggata ttcggcgtgt aggcgtgaac
    91081 tgttccgacc tcgtatacgc gacaggttta cagcttaacc tttttgaaga tcctgaaaag
    91141 cagataaatg aagcgaaagt tgattttgtt gtcgatacga ttagaaaaaa atacggcttt
    91201 caatcaatca tccatgcgca tagtctcttg caaggaggac gcgccatcgc acgtagttct
    91261 ctcgttggcg ggcatgcagg tggtatgagt ggattagaag gtggaaaaga tgagacggac
    91321 agcaaaaaaa ttcattgact ataacgagta tcgagatcgg ccatttggat taaaatgggg
    91381 gactgctttt gcgatggaag aactagtcaa cggtatcaaa aaaatgaaga agaagcacgc
    91441 aaacagcctc ttcctaaaca acaaatgaca ttgcaagaaa tcgatagtat actgacagaa
    91501 gcctttttat ctcgtaaagc agtaatcatt cagctcaatt taaaagatga atttggaaga
    91561 ctcttggatg aggtcgaagg gcacttcatt ggtgaagcct atgaagatta ctttatgatt
    91621 gaccatcagc cgatattgtg ggaagacgta cggtatattg agttaaagga aacaaaaaaa
    91681 tggtttgtga catgagaggt tgtgacagaa gtgatcaact tcaaaaaata agaaaggatt
    91741 tttcgaaaat tgcttcttaa atttttgtgg aattttggct tatttttcga agaagcaact
    91801 tctggaacaa cgtatattag atatcagagg ttgtgaaaaa gtcgcttagt tttgagtaac
    91861 aagccaaggc gctgatttca caacctctgt ctatctatcc atctattcgg taagattcat
    91921 gcatgacgtc ttctttattt agtatgtaga gccctttttc ttctgaccat tcaacataaa
    91981 tcagctgttt tacatctgca tagccttctt tcttcaagtt ctccataagc catgcttcag
    92041 tcaattgatt ggactgaagc actttatgct gaatttgccc ttcatctaca agcaggatcg
    92101 ataaagcttt gtcttcatct gattttttca atacactcat ttgaccggca ttctctagga
    92161 taacatgttt ggcattttca agaaaaaagc attcttgctg tcttaagatt gctcgcaact
    92221 gctccatctc gatatggtta tttgtcaact ctgtcatgtt gatgacgccg tctttgatta
    92281 gaacagatgg ctcgcctttg acccaacgat tgaccttttc atttttttgg acgatacgtt
    92341 cgataccata aatcattact gcccaaagcg caatggcaaa taggacatgc cctacatgga
    92401 cattgtcatc ataggttgat tcttctaaga tcccacctaa aacaagcgta tagactaaat
    92461 caaacggtgt gatttctgac atggatttct tcccaagcaa acgtaccact aataataaac
    92521 cgatcaaccc gaacaccagt ttcattgtaa ttgaaaaaat cattttttct ccccctaact
    92581 atctgttaat taaattatat atgctttctc acgaacacgc atggaatata caaggaagaa
    92641 atgtatcctt ttactaaaaa aaggactgtg acactgtagt gacccccaaa agttagactt
    92701 ttttggagtg gagaaatcca ctcctttttt ttatgctcta aaatgatatt tttacacaac
    92761 cgttacggtt gttttacgaa attggacg
//
LOCUS       DASHHB010000004        78717 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.004, whole genome shotgun sequence.
ACCESSION   DASHHB010000004 DASHHB010000000
VERSION     DASHHB010000004.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78717)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78717)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78717
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.004"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(1014..1304)
                     /locus_tag="U2K71_000300"
     CDS             complement(1014..1304)
                     /locus_tag="U2K71_000300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314419.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
     gene            1706..1942
                     /locus_tag="U2K71_000301"
     CDS             1706..1942
                     /locus_tag="U2K71_000301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314420.1"
                     /translation="MQFLEPKNKNAKSVDWEISEQVRVIVKQYAEYAERTESEAVDEF
                     LLNILDDKKFIEWIANKRSNKRIVEKMGIKDRVG"
     gene            1949..3901
                     /locus_tag="U2K71_000302"
     CDS             1949..3901
                     /locus_tag="U2K71_000302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEM7314421.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="U2K71_000303"
     CDS             complement(3973..4470)
                     /gene="dfrG"
                     /locus_tag="U2K71_000303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEM7314422.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(4797..5489)
                     /locus_tag="U2K71_000304"
     CDS             complement(4797..5489)
                     /locus_tag="U2K71_000304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEM7314423.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            5884..6507
                     /locus_tag="U2K71_000305"
     CDS             5884..6507
                     /locus_tag="U2K71_000305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM7314424.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEQP
                     VVQETVQTEKQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTATNGRYI
                     GRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            complement(6591..7313)
                     /locus_tag="U2K71_000306"
     CDS             complement(6591..7313)
                     /locus_tag="U2K71_000306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEM7314425.1"
                     /translation="MLSDAHCHLDGSQSLALLQQEHTILTIINCDSPEEWKENRQLAA
                     SKTQALSYGIHPWKADSYTFEQVEPFLKKARIIGEIGLDNIWTNVPMTTQKKVFERQL
                     AFAAINEKPVVLHTKGCEKEILNYIQAYPNHYLIHWYSSLDYQQDYIDLGCYFSIGLD
                     LKKNPAVWQLAKAVPIDHLLIETDGLHAAEWALGGIFSEKDYVSLLEEQLKTIAEIKE
                     LSVEEVKKNTYQNLERFIRLGK"
     gene            complement(7303..8040)
                     /locus_tag="U2K71_000307"
     CDS             complement(7303..8040)
                     /locus_tag="U2K71_000307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314426.1"
                     /translation="MSIQISHLNVVIENLKILEDIHFTIPKDTFTSIVAPSGAGKSTL
                     LKTLTGVQPMTSGTIKVDDQKVTGLHTAFSYMPQEDMLLPWLSVYQNVTLYQKINHLT
                     INEEQVRDYLDIFGLNGYEHFLPEQLSGGMKQRTALLRTIMNPSSYLLLDEPFGALDA
                     MTRGQMQDWMLKLPKKAKRTTLLVTHDIEEAIYLSDRILVLSARPAHVIAEIQVPEKR
                     RSREWLLQQSELKQTIYQLLAGELDVK"
     gene            complement(8052..9044)
                     /locus_tag="U2K71_000308"
     CDS             complement(8052..9044)
                     /locus_tag="U2K71_000308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM7314427.1"
                     /translation="MKKLWLLLPLLLLLSACGTAKETSSKQEIKDLKKVTLVLDYVPN
                     TNHTGIYLAKEKGYYKEAGLNVQIIEPGDNSTSIGLVGADKAQFGVSYQEDVTYAHAD
                     GQNIPVKAIATVIKHNTSGFATLSDSNIHSPKDFEGKTYAGWQSPSEEAVLKAVMEKD
                     GGDFSKLTMVGSNGEGPESLGKSSDIQWYFEGWDMIKAKEAGIELNYIPLKELDERLD
                     YYTPVIITNDQLIKSDPELVQSFMDATKKGYQEAIKDPNDSAKLLQKYAKENDRTFLE
                     ESQAFLSKNYTDDPENWGLMEEKVWRNYTAFMQENGLIKQDVPSQELFTNQFIK"
     gene            complement(9059..9802)
                     /locus_tag="U2K71_000309"
     CDS             complement(9059..9802)
                     /locus_tag="U2K71_000309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314428.1"
                     /translation="MKKNLYPAVSMAFILIIWQIVVIVWKIPAFILPGPAAVFSALAA
                     DAQVLLSHSVTTLNEAVLGLVIASVLSFITALAMDYWKFLEMSFYPLLVISQMLPIMV
                     LGPLLTLWFGFGMLPKVILVVLISYFPIVVSFSDTLRKVSKEQIIFLKTMGADTGKIY
                     RIYKIPAGLSGFFSGLKVAATYCVSGAVVGEWLSAQAGLGYYMIRVKNSYQIDKVFAA
                     IICVILLSLLLNGACSLLKKGYYRMLYIF"
     regulatory      complement(9876..9980)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(10043..11365)
                     /locus_tag="U2K71_000310"
     CDS             complement(10043..11365)
                     /locus_tag="U2K71_000310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM7314429.1"
                     /translation="MKTYDAIIIGSGVSGLSAAYGLKEAGKTVLVVEEDLWGGTCPNR
                     GCDPKKVLLSAVEARNRVKQLSGKGFNEIPTANWEELQKFKRTFTDPVPESRKKQLAE
                     AEIDHLSGTARFLDDSSIEVNEEVFHADYLVLATGQRPTILPVEGKEYLKTSADFLSL
                     PVLPKEIIFIGGGYIAFELATIANAAGSKVTIVHHNQRPLKEFEASLVEEAVHQMEAS
                     GIQFAFGVETQKIISEGTRYRLVGKETELVADMIFCATGRQPNTESLALEQANIVFDK
                     HGIAVNDYLQTSNPKIFACGDIVSRKTPKLTPVATFEGNYVAKRITDATSEPIKYPII
                     PTIVYASPKLAEVGVTKSHASSSDQVVEMDLTSWFTYHRVNEPVAKAELTFDQQNYLI
                     GAAVISEQADELIDDLTLVINQKLTKKELDSYIMGYPTLASDLSYLLK"
     gene            complement(11352..12317)
                     /locus_tag="U2K71_000311"
     CDS             complement(11352..12317)
                     /locus_tag="U2K71_000311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314430.1"
                     /translation="MTKLIEFKHVQKKYDGKYVIDDLNLAIKKGEIFVLVGPSGSGKT
                     TTLKMINGLSKPSAGDIYFKGKSLNEYNLQKMRWNMGYVLQQIALFPTMTVKQNIEVI
                     PEMLGWEKQKRADRVDELLQKVGLSPDIYRDRMPRELSGGEQQRIGIIRAIAASPDVI
                     LMDEPFSALDPISRNSLQELVLSLHEELGTTIVFVTHNMEEAIKLGDRIAFMKDGEII
                     QCDTPEQLLMNPKNDYVRHFFDEPKQTKEWRVEDLVVNGYFLNEIPENARQQVCFDTP
                     MKEVYSLLSVYPSIVIVEKQRSIGSLTSKEIFAFLSREEEGNENI"
     gene            complement(12314..13852)
                     /locus_tag="U2K71_000312"
     CDS             complement(12314..13852)
                     /locus_tag="U2K71_000312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate-binding
                     protein"
                     /protein_id="HEM7314431.1"
                     /translation="MNNFIQTLSERKGELLEAAFQHLSISLISLLIAALIAIPLAIWA
                     ANHKKMAEVLLQITGVLQTIPSLALLGLLIPFVGIGTVPALIALVIYALLPIFQNTYI
                     GLAEIDPSIEEAAVAFGMSRMRRLLKVELPIALPVIISGIRTALVLIIGTATLAALIG
                     AGGLGTFILLGIDRNTPVLTLIGAISSALLAIIFSGLIRWLQHKKPRYSIITLAAVVC
                     LFGGVSLYQSALFKNETITIAGKLGAEPEILIQMYKELIEDNTKTEVELKPNFGKTSF
                     LFNALDQGQIDIYPEFTGTVLESLVKVPEAMKSADLTKEKTYDEAEELLNKQFDMTLL
                     KPMAYQNTYALAVKRSFANEHQLKKISDLAAISDQIKAGFTLEFIDRSDGYKGIQEAY
                     GLNFSSVQSMEPSLRYQAINNGDVNIVDAYSTDSQLKEYDLVTLEDDRGLFPAYQGAP
                     LMKKDFAKEHPEIVKALNKLSGKITEDQMIDMNYQVNVEKKKPTEVARIFLKNEGLVK
                     EEDQ"
     gene            complement(14268..14555)
                     /locus_tag="U2K71_000313"
     CDS             complement(14268..14555)
                     /locus_tag="U2K71_000313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314432.1"
                     /translation="MRAADLLTILTKSPKKTEYQGVFLAQEQKLVPVTMVQINKANQF
                     IFLFEHGKAPLAMKDIFLFLMKNREKAVYYQSKEELIPLYGVKEIENKVVI"
     gene            14689..15357
                     /locus_tag="U2K71_000314"
     CDS             14689..15357
                     /locus_tag="U2K71_000314"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM7314433.1"
                     /translation="MKKLNLFTELCGIILGAGIYGLAVTGINIPSKLADGGVTGIALL
                     LHNLFGFAPSVTSLLFNLPLLLISLFLFGKHAFLRTIVGTFALVFFLHLWESLHIHFA
                     VDSLLINSLMTGILSGIGCGLVFRFGGSTGGTDIIYQAIEKYFHIKIGKSLFLITFGI
                     LVVSLLYLDLTHFLYTLLSCSILSYTLNKVKYLEIGSVLPQLEAKQTTETFEPLDPLE
                     QLEH"
     gene            complement(15648..16388)
                     /locus_tag="U2K71_000315"
     CDS             complement(15648..16388)
                     /locus_tag="U2K71_000315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314434.1"
                     /translation="MKEIRRKHFRFPAYDDELGVKLTNDKKRVLFDGQEDLLTEQNQS
                     PFEQMKEFHFTNTNTEKIRRKRRATNGLKSAPRKENLEKHKGKLPDYTGHYQSEITST
                     GRKKLFGDSSRSVRQSETRTESKHTTRNASVDTKVRERSYFVPKYVPASIIPDPRESK
                     ISEDHLIESMKKTDDSYLLFDTEPTPYQEKRNGEPSVRTFNRTKTLEETKQSEEKKSK
                     KSHGILERSLQGLIDDQGNSLGENSYFK"
     gene            complement(16518..17465)
                     /locus_tag="U2K71_000316"
     CDS             complement(16518..17465)
                     /locus_tag="U2K71_000316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7314435.1"
                     /translation="MPTLADVAKRANVSKMTVSRVINHPEQVTDELKELVFSAMAELD
                     YRPNIAAKALVTNRSQIIKLFILEEIDTTEPYYMNLLMGIAKRIGKAHYSLQLVTNDA
                     FDVGSCDGYIITGVRKKDFDWIDRLEKPVVLFGENSEGYDYVDSDNEYGTAKATQYAL
                     ERDYQTIIYIGIEVDEPFEISRETGYLSVMKEVDLPSKILRFPNHSTEVERYMTENWA
                     DFTDKTCFVCSSDRLALGAVRGIINAGGHLPEEFGVIGFDGVFLDQISSPKLTTIKQD
                     IIRMGEVCGEMLLKKIEEKGASQGYRRFFPELVIRETTN"
     gene            complement(17594..18358)
                     /locus_tag="U2K71_000317"
     CDS             complement(17594..18358)
                     /locus_tag="U2K71_000317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEM7314436.1"
                     /translation="MNPIILKRSVHHIPVLEVVLKELREERIPVVIYYHGWQTSKELV
                     LTQGRKIAQQGIRVILPDAMNHGERKQPVSSIPSYTFWDSIYGNLFEFDTLIKHLEKR
                     GLLHDQLAVGGVSMGGMTTCALLAKHPHIVAGVCLMGSPAPLEYGKEIVRRASEYNYV
                     VPKDYFDLISWVKSYDLSLQPEKLAGRPLFFWHGQADEKIPYIQTERFVEKNRHKSYG
                     KEIVFRSSKTQRHMVQIPLMEEASTFLAKNLIKKYK"
     gene            complement(18362..18751)
                     /locus_tag="U2K71_000318"
     CDS             complement(18362..18751)
                     /locus_tag="U2K71_000318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314437.1"
                     /translation="MKTVNRVFCASHVLSLLLSIYLGVYSIQQPIEVENIVFQMSLTD
                     GLILSVFFFLILFIGNIFGSVASFLNFSIYPLLSLALGVVGLCSLCLFPEPFSPAFIL
                     FGTLNLFQTTVGAWLLWRSGNLMKIGE"
     gene            18886..19428
                     /locus_tag="U2K71_000319"
     CDS             18886..19428
                     /locus_tag="U2K71_000319"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2179 domain-containing protein"
                     /protein_id="HEM7314438.1"
                     /translation="MIDPKMLGMIFLINFAYITLNTLRFMLTMKGYRLIAPLVSMVEI
                     TIYIVGLSLVLDRLDNPLNLFVYALGYAVGISVGILIEDKLALGYIMVTTILPANSAE
                     DKSLPGILREHGYGVTQTSAMGLEGERLVLEILSPRKSERELYRLIKEIEERAFIISY
                     EPKYISGGFWTKKVRKRQGK"
     gene            19576..20208
                     /locus_tag="U2K71_000320"
     CDS             19576..20208
                     /locus_tag="U2K71_000320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314439.1"
                     /translation="MEIQRRTTNFLHLGSFFATLTILFFLCYSYLKTSHAPSSALGWL
                     LLDHSDITEAEENPFFLILSSLCGLLFSVYFGAYFFHRHPGTLNEASSVLIAFKTFSF
                     LAILFYSFNHNTLFFFIMNGLVVILSIFTYFIFSLIFSQLKCPLFFRLVPVTCFIYSL
                     FSFFVLALMPLTNPSTIQVAGNLLDMLFIVSLGVFMWQRKNKQNKKHFEA"
     gene            complement(20342..20467)
                     /locus_tag="U2K71_000321"
     CDS             complement(20342..20467)
                     /locus_tag="U2K71_000321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7314440.1"
                     /translation="MQKNGEKCGMTKEVVIRKVRFLNNQYYDSVKYGILWEELAD"
     gene            complement(20601..21494)
                     /locus_tag="U2K71_000322"
     CDS             complement(20601..21494)
                     /locus_tag="U2K71_000322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1803 domain-containing protein"
                     /protein_id="HEM7314441.1"
                     /translation="MNYYSKNKENFYQKLTDDPLFSSLTDYLYEHREQETILRELKKE
                     FSQNKFSHFLDLLIDAGLIKREERRYHLNFPIFDPKDYLQQATSAAETIAEQLKRLSV
                     DEQKLAMGEVIWAYCFEDERKEAYFYGVRNSRETELLRTTAGNEKYRFITLSSIEHFP
                     LTLANYFFVQKNQLPVTKAFKELAELIGDVNESYFFDQIEVIVDRIRKNKYKNRRPSI
                     FHQSLLVTNTIKEEESFTLELPIVEKNNFEIELPTLDPSLTMEEMAFLKRQIFSELSK
                     KFIPHAFSYIKEYGTVLVSKT"
     gene            complement(21676..22602)
                     /locus_tag="U2K71_000323"
     CDS             complement(21676..22602)
                     /locus_tag="U2K71_000323"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese-dependent inorganic pyrophosphatase"
                     /protein_id="HEM7314442.1"
                     /translation="MSKILVFGHQNPDTDAIGAAIAFSHLQNELGKETEAVALGTPNE
                     ETQYALDHFQLSAPRVVESAKAENADVMLVDHNEFQQSISDISEVNILAVVDHHRIAN
                     FETANPLYYRAEPVGCTSTIILKLYKENQVTIPKEIAGIMLSAIISDTLLFKSPTCTP
                     EDEAAAKELAAIAEVDLNTYGLEMLKAGTNLSDKSAAVLLDLDAKSFPMGSSNVRIAQ
                     VNTVDLNEVMDRQAELEAAMEDEMKANGYDLFVLVVTNILDSDSELLVAGKGKEKVEE
                     AFQTTLTNNRALLKGVVSRKKQVVPQLTAVFS"
     gene            complement(22687..23448)
                     /gene="pflA"
                     /locus_tag="U2K71_000324"
     CDS             complement(22687..23448)
                     /gene="pflA"
                     /locus_tag="U2K71_000324"
                     /EC_number="1.97.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate formate lyase-activating protein"
                     /protein_id="HEM7314443.1"
                     /translation="MEEKTIGYVHSIETFGSVDGPGLRFVVFMQGCRMRCQFCHNPDT
                     WNIGGGKEYTADELLDKAERFRPYWGDKGGITVSGGEPLLQIDFLIELFKKAKERKMH
                     TTLDTCGKPFTYEEPFFSRFQELMKYTDLLLFDIKHIDNEEHKKLTHWDNENILEMAQ
                     YLSKINKPVWIRHVLVPERSDYDEYLIRLDNFIQTLSNVDRVEILPYHTMGKYKWETL
                     GLKYPLEGIEPPTKERVENAKRLLHTEEYTKYLTR"
     gene            complement(23692..25923)
                     /gene="pflB"
                     /locus_tag="U2K71_000325"
     CDS             complement(23692..25923)
                     /gene="pflB"
                     /locus_tag="U2K71_000325"
                     /EC_number="2.3.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate C-acetyltransferase"
                     /protein_id="HEM7314444.1"
                     /translation="MEQWNGFKGTKWKEGVDTRDFIQNNYTEYRGDDSFLEGPTEATE
                     KLWDELQKLNDIQFERNGVYDMDTEIVSTITSHDAGYLDKDLEKIVGLQTDKPLKQAF
                     MPFGGINMANNALKSNGYTPNDELTHIFTDWRKTHNQGVFDAYTAEMRAARKNKIITG
                     LPDAYGRGRIIGDYRRVALYGIDYLMEQKKKDHDMTGYHTMSEEVIRLREEITEQYRA
                     LAQMKEMAAKYGYDISKPAANAKEAVQWLYFGYLAAIKSQNGAAMSIGRISAFLDIYI
                     QRDLDNGVITEKEAQELIDHLVMKLRMVKFARTPEYNQLFSGFPIWATLSIAGMGLDG
                     RSLVTKNDFRLLHTLTNMGPSPEPNLTVLYSEHLPEGFRTYASRIAIESSSIQFENDN
                     QLREQWGSDDCAIACCVSGTVVGKDMQFFGARANLAKTLLYAINGGVDEVTGAQVGPE
                     YRPITSEILDFEEVKHNFMNMMDWLAELYVNTLNIIHYMHDKYSYEAAQLALMDSDLQ
                     RTFATGIAGISHAADSLSAIKYAKVKAIRNEKGIAVDFEVDGDFPKYGNDDDRADELV
                     SWILEYFMGQIKRHKTYRNSKPTTSLLTITSNVVYGKNTGNTPDGREAGKPLAPGANP
                     SYNAEQSGLLASLNSTAKMNYGCALDGISNTQTINPSGLGKEQDTRIENLRNVLDGYF
                     SKGAYHLNVNVFSNDLLRDAMENPMKYPNLTIRVSGYAVKFRDLTREQQLDVLMRTSH
                     DRM"
     gene            complement(26200..28650)
                     /gene="parC"
                     /locus_tag="U2K71_000326"
     CDS             complement(26200..28650)
                     /gene="parC"
                     /locus_tag="U2K71_000326"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit A"
                     /protein_id="HEM7314445.1"
                     /translation="MENRQEVQELTLEEVMGDRFGRYSKYIIQERALPDIRDGLKPVQ
                     RRILFAMNKDGNTYDKGFRKSAKSVGNIMGNYHPHGDISIYEAMVRMSQDWKLREVLI
                     EMHGNNGSMDGDPPAAMRYTEARLSKLSGEMLADIEKETVDLVWNFDDTEKEPTVLPA
                     RYPNLLVNGSTGISAGYATEIPTHNLAEVIDGTIYMIDHPQASLEKLMEYIPGPDFPT
                     GGILQGKDEIKKAYETGRGKVILRSKTKIEAMKGNKQQIVITEIPYEVNKATLVKKMD
                     EIRLNKKIDGIAEVRDESDRTGLQIVVELKKDANAQGILNYLFKNTELQINYNFNMVA
                     IDHMTPHQVGLKDILRSYIEHRKQVITKRSQFDLAKAQKRQHIVEGLMKALSILDEVI
                     ATIRESKDKKDAKKNLVDVFQFTEEQAEAIVTLQLYRLTNTDITELQKESESLIAQIT
                     ELNKILSNDKELFSVMKKELREVKKNYSSARLTTIEDEIEEIKIDTQVLVAQEDVIVS
                     VTREGYVKRTSLRSYSASKPEEIGMREGDYLLYSGELSTLDHVLLITNKANVIYRPVH
                     ELPDLKWKDAGEHISQTISNLSVDESILAVFPYQKIQAEKTFVFISKNGLIKQTRMTE
                     FEPWRTYKSRPLSCMKLKAEEDELIAVYLKEDQPGLDVFLVTHQGMGLRYPLTDVPVV
                     GAKAAGVKSMNLKDGDRIINGLLVLAEGDTPIVIVTQRGAVKRMLAQEISQTSRAKRG
                     VTVMRELKKNPHRIIYMSEGRSKEITLMNQKGQQLTIDPTDFPIGDRTSNGSFAMDEK
                     KGGEVIEVIDAPEIFIES"
     gene            complement(28662..30707)
                     /gene="parE"
                     /locus_tag="U2K71_000327"
     CDS             complement(28662..30707)
                     /gene="parE"
                     /locus_tag="U2K71_000327"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HEM7314446.1"
                     /translation="MAKKVNNEYNDSSIQVLEGLEAVRKRPGMYIGSTDSRGLHHLVY
                     EIVDNAVDEALSGYGSEIDVTIHEDNSITVADSGRGMPVGMHASGIPTVEVIFTVLHA
                     GGKFGQGGYKTSGGLHGVGASVVNALSKWLIVTIVRDGVEYQQKFKNGGKPDGTLKKI
                     GKTKKANGTTVHFLPDDTIFSTTKFSYEILAERLRESAFLLKGVKISLSDLRGEEPVK
                     EIFHYEEGIKEFVDYLNEEKDTLTPVVYFSGEKEGIEVEVAYQYNDGYSENVLSFVNN
                     VRTKDGGTHEAGMKAAMTKSYNEYARKVGLLKERDKNLEGSDFREGLAAVLSIRVPEN
                     LLQFEGQTKEKLGTPVARTVVDNVISEQMGFYLQENSEMSQMLVRKAIKAREAREAAR
                     KAREESRNGKKRKKGESLLSGKLTPAQSRNPKKNELYLVEGDSAGGSAKQGRDRKFQA
                     ILPLRGKVINTEKAKMQDILKNEEINTMIYTIGAGVGPEFSIEDCNYDKVIIMTDADT
                     DGAHIQVLLLTFFYRYMKPLIEAGKVYIALPPLYKVSKGQGKKQVIEYAWTDDELAAM
                     IKKVGKGYMLQRYKGLGEMNAEQLWETTMDPTSRTLIRVRIDDAAQAERRVTTLMGDK
                     VEPRRKWIENHVQFTLEEDGSILDKKEDTEISPSVSNDLLDEERADKNENNQLFEVE"
     gene            complement(30873..31307)
                     /locus_tag="U2K71_000328"
     CDS             complement(30873..31307)
                     /locus_tag="U2K71_000328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HEM7314447.1"
                     /translation="MSVKNPTQETIFNYLEEAKNIAVVGLSNKPERTSYMVAQVLQDN
                     GYKIIPVNPLLAGEEILGEKVYANLTEIDDPIDIVDVFRRSEFLPEIAGDFLKTDAKV
                     FWAQLGIENKEAAEMLRKAGRTDIVMDRCIKIELGKLKENQK"
     gene            31434..32072
                     /gene="plsY"
                     /locus_tag="U2K71_000329"
     CDS             31434..32072
                     /gene="plsY"
                     /locus_tag="U2K71_000329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HEM7314448.1"
                     /translation="MKLILLLILAYLLGSIPSGVWIGKLFFKKDIRQHGSGNTGTTNT
                     FRVLGKTAGIVVLLMDILKGTLATCLPMIFHLSGINPLWFGVCAILGHTFPIFAGFKG
                     GKAVATSAGMLLGFNPVFFVYSCIIFIVSLFCTSMVSLSSMISAVLITLSTIVLPYVA
                     PVILAKPNWLLTIIAFLVSSFIFYRHKDNIKRIRNHTESRIPFGLNSSKKKS"
     gene            complement(32204..33079)
                     /locus_tag="U2K71_000330"
     CDS             complement(32204..33079)
                     /locus_tag="U2K71_000330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HEM7314449.1"
                     /translation="MTVIIQNDQLIAEIAEHGAELISLKSKEHGLEYIWQGDPAYWGR
                     HAPVLFPFVGRLKDDQYVYQGKTYQMGQHGFARDMDFEVIEHGQEMASFILRSTPETL
                     EKYPFDFELVISYELGGDGITVHYQVANTGEKEMFFSIGGHPAFNVPLEEGLSFEDFY
                     LAFSPLKSRLRLPLKGPFIDMDQKTLGQTNTNLALNRELFEQDALVFETRGLNAYAIR
                     SEKSSHSVTLSYKDMPYVGIWSPYPKEAPFVCIEPWCGIADTVNSSGNLMEKVGINQL
                     GAHEIFKTKYSITVK"
     gene            complement(33161..33952)
                     /gene="codY"
                     /locus_tag="U2K71_000331"
     CDS             complement(33161..33952)
                     /gene="codY"
                     /locus_tag="U2K71_000331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-sensing pleiotropic transcriptional
                     regulator CodY"
                     /protein_id="HEM7314450.1"
                     /translation="MTDLLTKTRKINTLLQQKNTFDMQADLPYDKMAVTLGGILDSNT
                     YIIGSDGVLLGYNERHDVNNERIKTMFVEKQFPESYTDTVAQLMKTEANIPITSDITA
                     FPVESREKYPFGLTTVVPIFGAGERLGTIILSRMEQSFADDDLVLAEYGATVVGMQIL
                     YQKSRSIEADVRSATAVQMAINTLSYSELKAVQAIFEALDGDEGRLTASNIADSIGIT
                     RSVIVNALRKLESAGIIESRSLGMKGTYLKVLNSRFKDELAKHSY"
     gene            complement(33970..35370)
                     /gene="hslU"
                     /locus_tag="U2K71_000332"
     CDS             complement(33970..35370)
                     /gene="hslU"
                     /locus_tag="U2K71_000332"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002409948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HEM7314451.1"
                     /translation="MYELKQTPRQIVSELDEYIVGQHAAKKSVAVALRNRYRRLQLDE
                     KMQQDITPKNMLMIGPTGVGKTEIARRLAKIVNAPFVKVEATKFTEVGYVGRDVESMV
                     RDLVENAIQIVEKEQYSRVYTSAKKNAEKRLVKILVPGIKKEQKQSTNQFEQMMNLFN
                     ASQQNTAEPQEEVTEDIRINRRTVLEQLQKGLLDSREVTIEVEDQKKPAPMMNNGLEQ
                     MGIDLNETLGALTPKKKVERTVTVKEALELLIKEESAKLVKDADIHSEAIKLAEESGI
                     IFIDEIDKITSKSQQSGEVSREGVQRDILPIVEGSQVNTKYGIIQTDHVLFIASGAFH
                     LSKPSDLIPELQGRFPIRVELDDLTAEDFVKILTEPNNALVKQYVALIGTENVSVTFT
                     KEAIERIAQIAFDVNRDTDNIGARRLHTILEKLLEDLLFEAPDMQMGEITITEAYVDD
                     KLGSIAANEDLSRYIL"
     gene            complement(35384..35932)
                     /gene="hslV"
                     /locus_tag="U2K71_000333"
     CDS             complement(35384..35932)
                     /gene="hslV"
                     /locus_tag="U2K71_000333"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HslU--HslV peptidase proteolytic subunit"
                     /protein_id="HEM7314452.1"
                     /translation="MVESPFHSTTICAVEKDGKFAMAGDGQVTMGESVVMKGTAKKVR
                     RIYNGEVVVGFAGSVADAFTLEEKFEGKLNEYNGNLQRAAVELAQEWRTQQSMQKLEA
                     MLIVMNDKEMLLVSGTGEVITPDDGILAIGSGGNFALAAARAMKNYAQNDMSAKEIAE
                     NALNIAADICIFTNHNIIVEEL"
     gene            complement(35969..36874)
                     /gene="xerC"
                     /locus_tag="U2K71_000334"
     CDS             complement(35969..36874)
                     /gene="xerC"
                     /locus_tag="U2K71_000334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HEM7314453.1"
                     /translation="MTEATWEQKFLRYLIVERGYSEKTKEAYEEDILHFKHFLEESGD
                     ADLLKAEYFTVRVYLSALADHRPSYSRNSISRKIASLRSFYQYLLKEEVIKENPFSYV
                     HLKKKNLRLPRFFYENEMQALFDSVAGDSPLDLRNRALLEVLYGSGIRLSECSSLSVS
                     DIDFDSEVMLIHGKGNKERYAPLGSFAQDALQEYFREGRQVLMTKYHEEHDIVFVNHH
                     GKPITPTGIEYVLNQMIKKSSLDSDIHPHMLRHTFATHLLNNGADLRTVQELLGHANL
                     STTQIYAHVTKESLQKNYRSFHPRA"
     gene            complement(37072..38448)
                     /gene="trmFO"
                     /locus_tag="U2K71_000335"
     CDS             complement(37072..38448)
                     /gene="trmFO"
                     /locus_tag="U2K71_000335"
                     /EC_number="2.1.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FADH(2)-oxidizing
                     methylenetetrahydrofolate--tRNA-(uracil(54)-C(5))-
                     methyltransferase TrmFO"
                     /protein_id="HEM7314454.1"
                     /translation="MEITKKTSKNNRKEVLSLKMVNVVGAGLAGSEAAWQIAQAGVPV
                     RLYEMRPVKSTEAHHTSNFAELVCSNSLRGNSLANAVGLLKEEMRRLNSVVITSADET
                     AVPAGGALAVDRDSFSETITRKVKEHPLVTVINEEITEIPEGITVVATGPLTSHPLAE
                     SIKAFNGSDGFYFYDAAAPIVDKATIDMDKVYLKSRYDKGEAAYLNCPMTEEEFKAFH
                     EALINAEVAELKSFEKEKYFEGCMPIEVMAQRGFKTMLFGPMKPVGLEDPKTGKRPYA
                     VIQLRQDNAAASLYNIVGFQTHLKWGEQKRVFRMIPGLENAEFVRYGVMHRNSFMNSP
                     ELLKPTYQSTKREDLFFAGQMTGVEGYVESAASGLLAGINAARLAKEMEPVEFPQETA
                     IGSMAYYITHAEGKHFQPMNANYGLFPELPERIRDKKARYEAIAQRALKANEAIKTEL
                     EKTAIPNE"
     gene            complement(38417..40495)
                     /gene="topA"
                     /locus_tag="U2K71_000336"
     CDS             complement(38417..40495)
                     /gene="topA"
                     /locus_tag="U2K71_000336"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002380330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HEM7314455.1"
                     /translation="MAYKYLVIVESPAKAKTIEKYLGRNYKVMASVGHIRDLPKSKMG
                     IDFENNYEPHYISIRGKGDVIKSLKAAAKKAQKVYLASDPDREGEAIAWHLAYLLGLD
                     LKEKNRVVFNEITKDAVKAAFKEPRTIDVDLVDAQQARRTLDRIVGYSISPILWRKVK
                     KGLSAGRVQSVALKIIIDREKEIREFVPEEYWSIDGNFKKARKKFKANFWGIDGKKKK
                     LPDAQSVKEVTARIDGTDFEVKKVEKKERKRNPANPFTTSSLQQEAARKLNFRTRKTM
                     MVAQQLYEGITLGKQGTVGLITYMRTDSTRIADTAKAEAAAFIEDTYGKEFSRHDHRK
                     IKNAQGAQDAHEAVRPTSVLRTPDELKQYLDKDQLKLYTLIWSRFVASQMTPAILDTM
                     KVTLEQNGVIFIANGSKIKFKGFMQVYVEGRDDGKEEKENILPELEEGDVVQSVDIEP
                     KQHFTQPPARFSEATLIRALEENGVGRPSTYAPTLDTIQRRYYVKLTQKRFEPTELGE
                     IVNSLICEFFPQIVDIHFTAEMEGDLDKIEEGTEAWVKVVDRFYKPFEKELTNAEEKI
                     EKIQIKDEPAGFDCDVCGHPMVIKLGKYGKFYACSNFPDCRNTKPIVKEIGVTCPVCH
                     EGQVIERKSKKNRLFYGCSRFPECEFTSWDKPVGRNCPKCDGYLVEKKVKGGKQVVCV
                     NGDYEEDIQK"
     gene            complement(40617..41474)
                     /gene="dprA"
                     /locus_tag="U2K71_000337"
     CDS             complement(40617..41474)
                     /gene="dprA"
                     /locus_tag="U2K71_000337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-protecting protein DprA"
                     /protein_id="HEM7314456.1"
                     /translation="MYQIEENLLKLAYTKGISSQGKWALANWMMQYPYKEIGFEDIIK
                     IGKITKHRELFRKSWQEIHANWGKIQSKQSFITCFDPKYPPQLLHLTYPPIVLFYNGD
                     LSLLSCNMLSVVGGRHTSALAKKTVYYLLKPVIEAGYVIVSGCAKGIDTYGHQAAISH
                     GGRTIAVIGTGIEQVYPAENRDLQEVIANDHLLLSEYPPQSGPKKHCFPMRNRIIAAL
                     SKGTCVFEAKKKSGSLITAQQALDLGRTVFSVPGNILTEHSTGCHHLIQDGAVCTVSG
                     EDILAEFKE"
     gene            complement(41530..42297)
                     /locus_tag="U2K71_000338"
     CDS             complement(41530..42297)
                     /locus_tag="U2K71_000338"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HEM7314457.1"
                     /translation="MTESIQSIKERLKSIQRIDDPTLESLRNDPRKGVQQALASFERR
                     LKKEALIKEKYEEMFCFEKQGYAQGHQWIAGIDEVGRGPLAGPVVAAAVILPKNEIIL
                     GLNDSKQLSEKKRELLYQQVQEKALAIGIGIVDQQTIDDVNIYEASKLAMIQAVEKLS
                     IVPSYLLIDAMTLDLPIQQEKIIKGDARSVSIAAASIIAKVYRDQLMKEYDVLYPGYG
                     FSHNAGYGTKEHLEGLERQGITPIHRKSFAPVKKYLQ"
     gene            complement(42290..43150)
                     /gene="ylqF"
                     /locus_tag="U2K71_000339"
     CDS             complement(42290..43150)
                     /gene="ylqF"
                     /locus_tag="U2K71_000339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YlqF"
                     /protein_id="HEM7314458.1"
                     /translation="MTIQWFPGHMAKARREVSEKMKYVDIVFELVDARLPLSSRNPML
                     DQIIQQKPRLVLLNKADLADPQQTKLWQEYFRKQGHLALAITAQESKGIKGLVPAAKE
                     ALKEKRARDAARGIKPRAIRAMVLGIPNVGKSTLMNRLVGKKIAQTGNKPGVTKGQQW
                     LRLGNELELLDTPGILWPKFEDEEIGKKLALTGAIKDQLVHLDDLAIYGLDFFARYYS
                     GRISDRYGLSIEQEEKLAPELLMDITAKRGYKEDYDRGSELVVHDIRQGKLGRYTLDR
                     CEEMEQKSDD"
     gene            43410..43808
                     /locus_tag="U2K71_000340"
     CDS             43410..43808
                     /locus_tag="U2K71_000340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM7314459.1"
                     /translation="MNSMVFVNFPVADVNRSVAFYEKLGFKKNEEFSTEEAGSMVWDD
                     TFWIMLLSHDFYKKFIKDKEIADNKKTSGALISFSVENADEVKKFAQIAKENGGTFHE
                     VDMGMPEEEMFSLEVQDLDGNTLEPVWMKM"
     gene            44087..44587
                     /locus_tag="U2K71_000341"
     CDS             44087..44587
                     /locus_tag="U2K71_000341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEM7314460.1"
                     /translation="MNARLLSTSALLLALDVILYKIAIGPAYFQITFGFLSMALMGYL
                     YGPIWAGLLEMLSNTLNFTIFGSGTFQAAFLITAFLGGFINGLFLYHKKINLPSVIAA
                     QLLIMIPVSLFINSWLMSVLFGADFKVIFLGRFIRNMIQIPIQIMVVYSFIKTLEARK
                     VFKRAV"
     gene            complement(44670..45221)
                     /gene="lepB"
                     /locus_tag="U2K71_000342"
     CDS             complement(44670..45221)
                     /gene="lepB"
                     /locus_tag="U2K71_000342"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM7314461.1"
                     /translation="MTKKQRYINRFWLVFKYLLVSVFLAFMLRGFLFIPVPVEGNSME
                     NVLKQGDMVVMEKFSEIRRFDIVVFQLADGTIYIKRVIGLPGENVSYQNDQLKINGKV
                     VKEPYLTKNLKSDHANASYTTDFTLQELTGQSKLPEDNYFVLGDNRRVSKDSRSFGTI
                     NKTDILGKARFVYYPLDEIKWIQ"
     gene            complement(45373..45600)
                     /locus_tag="U2K71_000343"
     CDS             complement(45373..45600)
                     /locus_tag="U2K71_000343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YozE family protein"
                     /protein_id="HEM7314462.1"
                     /translation="MNRSFFHYLMTLRGGGTHDALSVFATEAGKDIQFPKHSSSYEEI
                     SNYLELNVDYLPSMDIFDEAWEKYLENNHDI"
     gene            complement(45597..46115)
                     /gene="msrA"
                     /locus_tag="U2K71_000344"
     CDS             complement(45597..46115)
                     /gene="msrA"
                     /locus_tag="U2K71_000344"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEM7314463.1"
                     /translation="MERAIFAGGCFWCMIQPFDTLPGIHTVMSGYTGGHVPDPTYEQV
                     KSQTTGHTEAVEIIYDPTLISYEQLVELYWQQTDPTDAFGQFEDRGDNYRPVIFYLTE
                     EQRIIAEKSKKQLAESGRFEQPIVTKIEPAQPFYLAEEDHQEYYKKHPENFKRNHARR
                     AAFIEENWRNEG"
     gene            complement(46135..46848)
                     /locus_tag="U2K71_000345"
     CDS             complement(46135..46848)
                     /locus_tag="U2K71_000345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpmS family protein"
                     /protein_id="HEM7314464.1"
                     /translation="MKTRSDVSSNKTKKTNYWKFAFLILLGVVLGSTVFLGSRIFANR
                     EPELPEIPALTERQGDPVLTINSNKEKVNQIISFFLSEYQKDSDIEYKFYLENEALLN
                     GTFEVLGFPIDFYLYFDPYVMENGNVQLKARSLSIGTLSLPIKDVMNMIKRNYKLPDW
                     IEVNTDDLTIMLRLDQFRMQNGMYIKADKIDLVNDDIRFSLYLPKDTSDASNNSNTNK
                     TSTSSTESKTKENKKSSDE"
     gene            complement(46851..47747)
                     /locus_tag="U2K71_000346"
     CDS             complement(46851..47747)
                     /locus_tag="U2K71_000346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEM7314465.1"
                     /translation="MKLTKRHLILVLFPLLIAIIIYAGLAASIPKAGPLMKPVTTVRE
                     DPNKKEKLHYIAIGDSLTEGVGDQTKQGGFVPLVANDLKDRYNLTAVETENYGVNGER
                     SDQILKRVKTKEEIRNNIKTADFITLTVGGNDLMKVIQNNLFGLTVNSFKKPLKNYQE
                     NVTELLEEFRSLNKTAPIYILGIYNPFYLNFPEITDMQTIVNNWNKGTETVTKKMPNC
                     YFIPINDLLYQGLNQEVGVTEEGTDQTTSTTENLNGIKNNVLYEEDHFHPNNLGYQLM
                     ANAVRDQLIETQNQWLEKEDDQ"
     gene            complement(47905..48747)
                     /locus_tag="U2K71_000347"
     CDS             complement(47905..48747)
                     /locus_tag="U2K71_000347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM7314466.1"
                     /translation="MTNVKIVTDSSCTMEKNVRDALGIRVMPLSIMIDGTVYPDDDQL
                     DGERFMEMMAKAKNLPKTSQPPIGEFISMYDELGEDGSEVISIHMTKGLSGTVEAARQ
                     ASNLTKTKVTVIDSDTTDQGLSFQVIRAAELAKEGKTVEKILEEVKKVQDNTKLYIGI
                     STLDNLVKGGRISRTTGLLSSVLNIRVVMNFAHSELIPVTKGRGKKTFDKWFNNLKNE
                     LKELPNVRQIGISHADAKELAQEFKEGLQELFPDMHIPVLHTNPVIATHTGKDAFAIM
                     YYVD"
     gene            48879..49532
                     /locus_tag="U2K71_000348"
     CDS             48879..49532
                     /locus_tag="U2K71_000348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin III family protein"
                     /protein_id="HEM7314467.1"
                     /translation="MEKTKFNRTYEILNEVFNAVTHGIGTGLSIAGLVVLLVKGAHLH
                     SSLHIVSYAIYGSMMILLFLTSTLFHSLIFTKAKKVFQVFDHSSIFLLIAGSYTPFCL
                     LSIRGWLGWTLFVLIWLFAILGIIYKALTLHKRERVSKVSTIIYIFMGWLCVVAAVPL
                     YHSLGMTGVILMVAGGLSYTIGAFFYSLNSIRYMHVVWHLFVMLGAGFMYFSVLFFT"
     gene            complement(49595..50122)
                     /locus_tag="U2K71_000349"
     CDS             complement(49595..50122)
                     /locus_tag="U2K71_000349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEM7314468.1"
                     /translation="MLAAIWAQDENGLIGKEDRLPWRLPNDLKFFKQMTEANTLVMGR
                     KTFEGMGSRPLPNRQTIVLTRDSSYKAEGVHVMHSVEDVLALEKETDGIFFIAGGSAV
                     YQEFLPFCTILYRTVIHHVFDGDAYFPPVDWSDWSLINTSQGEMDEKNRYPHQFETYQ
                     RNENPVKSGTGELTI"
     gene            complement(50142..51089)
                     /locus_tag="U2K71_000350"
     CDS             complement(50142..51089)
                     /locus_tag="U2K71_000350"
                     /EC_number="2.1.1.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidylate synthase"
                     /protein_id="HEM7314469.1"
                     /translation="MEQAYLDLGRKLLEEGHRKEDRTGTGTLSLFGYQMRFDLQKGFP
                     LLTTKRVPFGLIKSELLWFLKGDTNIRYLLQNKNHIWDEWAFERYIKSPDYNGPDMTD
                     FGHRCLTDPEFNEEYKKQSKLFCEKILTDDSFAETYGDLGHIYGYQWRHWETKDGGFI
                     DQIKQVIEAIKKTPDSRRLIVSAWNPEDVPSMALPPCHTMFQFYVQEGRLSCQLYQRS
                     GDVFLGVPFNIASYALLTHLIAHETGLAVGEFVHTLGDAHLYINHIEQMKEQLSREIR
                     AFPTLVLNQEKQSVFDFEMEDIQLEGYDPHPAIKAPIAV"
     gene            complement(51101..52987)
                     /locus_tag="U2K71_000351"
     CDS             complement(51101..52987)
                     /locus_tag="U2K71_000351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM7314470.1"
                     /translation="MLKELKVTDLKKTYGEKDLFDQISFLVHEKDRIGLIGTNGTGKT
                     SLLNIIAGIDSSDGDRQTVFYPTDYRIGYLSQAAEFSDELTVLQAVFQGNSPLIQTVR
                     AYEEALIELGENGEDPDVQKRYAKAEEQMNKEDAWTTDTNAKIILQKLGISELDKKIS
                     TLSGGQKKRVSLAQVLIDEPDLLLLDEPTNHLDYEAIEWLENYLKQYRGALLMVTHDR
                     YFLDRVANRIFELSFGKLYEYKGNYEAYVLEKAERDRVEVEQEEKRKRLYKQELAWMR
                     AGVQARGTKQQARINRFEDLKENLYQVNQEDDLELNLATQRLGKKVLEIKDGSYRIND
                     QTLLEHLDLLIQSRERLGITGKNGAGKSTLLNILAGRIPLDSGTMSIGETVHLAYYTQ
                     ENEEMAPDKRMIAYLQEAAEEAKTADGSQIGVAELLERFLFPRFMHGTLIGKLSGGEK
                     RRLFLLKLLIQQPNVLLLDEPTNDLDIATLTILEDYFRSFPGAVITVSHDRYFLDKVA
                     DKLLVFQGNGKQELYYGNMSSYLLKQKETQQPAEKAKPKIQSKEPAEKKKLSYMEQKE
                     WETIEDEIAELEEKISLLQEEMNHQGDNFTRLQELQNDVSETEAQLEEKMARWEYLSE
                     WVED"
     gene            complement(53147..55225)
                     /locus_tag="U2K71_000352"
     CDS             complement(53147..55225)
                     /locus_tag="U2K71_000352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HEM7314471.1"
                     /translation="MEFAELVIVFAVTITLSNIASRILPMIPAPLIQIFLGVVLGLTE
                     WGQSIDFEPELFLVMIIAPLLFREGEKADISSILKNFGTILFLAFGGVILTLVGVGAT
                     LSFLLPSVPLAACFAFGAALGPTDAVAVSSLSGRVNIPKKAMHILEGEGLLNDASGVT
                     AFQFALGALITGSFSAVNAGMSLVVSSIGGALIGFLLVWFKQKIIHLIEKASAQDVTG
                     YLLIELLLPFLAYVLAEAFDVSGIIAAVAAGILQASGFRKISVFDAELSSLSHSTWTT
                     IAFTLNALVFIFLGIELTQVFSPVWGDGLYPNGLLLAIIVLISVMLFVIRFISISLFY
                     VFKDGSKKFKKQLNEILILTFGGVKGTVSLATIFILPFSINNMMFYQRSLLLFLTAGV
                     ILVTLVIGIIVLPMLTETEEAKSTDLNALMILEEVVEVLRKEIKEIDRNTKEFLATEA
                     VIENYQERIRDLYLEDLTDDEKQEVQEIQALILSIERDGLDESYRSGKLSSNGYRFYS
                     RFLSRFEHSITSQILSFIGFWFIVVRRLMRIVLHPKMFWQRRYADRQTFISDEDIQEI
                     RETYQKNTQLIIESLDNLTDIYDETMVQFFIQQRKLEGIKMNSGNLISSWMIQQDSLF
                     TKKMLRGYYLERKIIDEYEVAEKITTFSANSYRRNINLLESYTMNKPSDNFSFRFAFR
                     AKSKKTSPIK"
     gene            complement(55238..55513)
                     /locus_tag="U2K71_000353"
     CDS             complement(55238..55513)
                     /locus_tag="U2K71_000353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314472.1"
                     /translation="MRESFEQQKKLLHDRYGALSMDDRRQILCKLRKRNILMYRQLER
                     LKHDLLRLESKRVQCELEGNQTQVEVVETKILKKKEQFLKMLTQNKK"
     gene            complement(55875..56330)
                     /locus_tag="U2K71_000354"
     CDS             complement(55875..56330)
                     /locus_tag="U2K71_000354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314473.1"
                     /translation="MSKEMTALKFYFRNGETWTINRRHIGDLWIKQITTSFGRINGSE
                     FVEIHPCAGFKIEIFHEGDAVATHDINLGGLEMGMFNRALKYEDIERMEILYRNGTPD
                     LVYFPYLDKGTEGLDNQYQSTKISEKTGNLYIVINPDQRVEDVYGEFFE"
     gene            complement(56484..57692)
                     /locus_tag="U2K71_000355"
     CDS             complement(56484..57692)
                     /locus_tag="U2K71_000355"
                     /EC_number="2.7.7.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HEM7314474.1"
                     /translation="MKLKQLPLEYQKAIPVLKKLENAGYEAYFVGGSVRDILLNQPIH
                     DVDIATSAFPEEIKQLFPKTIDIGIEHGTVLVLENDEQYEITTFRTESTYQDYRRPDH
                     VEFVRSLAEDLKRRDFTINAFALKEDGEIIDLFDGLQDLEEQILRAVGNPHERFHEDA
                     LRMMRGLRFVSQLGFKLEEETFYAIKENHELLGKISVERINVEFIKMLLGNERAAGIH
                     AFIETECYICCPGLKEEGEALLRFAELPSVPLKEESQAWALLISQLDLKENEIRSFLK
                     SWKCSNQMIKDVQALVQGLRQRETGALEPFELYFLGKKHALQVEELMIYFDETPQMDA
                     VGESYDRLPIKSLSELAVDGKILLEETGKKPGKWVSEALQLAEKAVVEQRIKNDKKQV
                     LDFLSKEKLI"
     gene            complement(57804..58136)
                     /locus_tag="U2K71_000356"
     CDS             complement(57804..58136)
                     /locus_tag="U2K71_000356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEM7314475.1"
                     /translation="MDKKRSLYSMQQEVDEYIQQFKVGYFSPLAQMARLTEEVGELAR
                     EVNHTYGEKSKKASEPVNSVAEELGDVLFVTMIMANSLNIDLTEVFEKNMEKFNQRDH
                     NRFERKDE"
     gene            58284..59159
                     /locus_tag="U2K71_000357"
     CDS             58284..59159
                     /locus_tag="U2K71_000357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM7314476.1"
                     /translation="MVKRKTVKEVLFIIIGTSIYAFGLVYLNIANHLAEGGVSGITLI
                     LRALFGIDPAYTTLLINIPLILLGGKILGKRSLAYTVLGTLSLSFFLWIWQRVPLEIN
                     LQHDLLIVSLLAGLVAGVGSGIVYRMGGTTGGSDIIARILEQNYGITMGRSLLAFDII
                     VLLASLTYIDLKRMMYTLIVSYVFSRVIDSILDGGYSAKGLLVVSNKSEEIAPLLMTG
                     LQRGVTFLSGEGAFSGNSKKIIYMVVSSSELTEAKRIVHEIDEKAFLSIINVHEVEGQ
                     GFTYLKPQTRLLKKL"
     gene            complement(59241..59822)
                     /locus_tag="U2K71_000358"
     CDS             complement(59241..59822)
                     /locus_tag="U2K71_000358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YpiB family protein"
                     /protein_id="HEM7314477.1"
                     /translation="MLIDVREKKNFLTWMVNHVSFSRREVFWILNYLANHEAILSNVH
                     FVEGANFADRGLQISDLSVEGEPMKLFLQGKEFTDTDQIFHEIRLNWKKPLYVECIFE
                     NAWQTREYLSILEDNPFSSWDSSVSEEDTELIERYFQEKEEEAKLKMLYAQIDQALED
                     GDRDAFLKLSDEVNRKILLQTQKKSNRVSEHNN"
     gene            complement(59828..61087)
                     /locus_tag="U2K71_000359"
     CDS             complement(59828..61087)
                     /locus_tag="U2K71_000359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEM7314478.1"
                     /translation="MNTNSEKMLQALSDEDLSQAQIFLEKALKEDPADILAELGEELL
                     AIGFLIEAKQIFEQLINQYPENDGLNIPLAEIAIEDNEIDLAFEYLEKVDKQSDYYPQ
                     SLLAIADLYQVIGIPEVSEAKLKEAAGLLPDEPLIQFALAELYFSVDRFKEAAVVYET
                     LLASGINEISGISMQERLGQSLSMQGEFEAAIVPLEEALKEERTDDRLFQLAFTNLQL
                     KENQQAINYLEELREVNPHYQSLYLYLGQALQEEEMIEEAQTVLEEGIKENPYQVELY
                     HLASENAFRLHDRQRAEELLLKALEVGDKQDETLLTLSNLYLDDERYEDVIQVINQME
                     ETANPYAEWNLAHAYNELEDFTVAAVHYEQAYHELSHEPDFLKEYAFFLREEGQLKRA
                     QELLTYYLTLEPGDMEALSLLDDLTER"
     gene            complement(61089..62135)
                     /locus_tag="U2K71_000360"
     CDS             complement(61089..62135)
                     /locus_tag="U2K71_000360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314479.1"
                     /translation="MELDYQVEDALTKVFYGETLVSQLRELSIDQPILFLTNQRYYDL
                     FADKINPLFANQANNDWYICANTQCNHLTELKNLLDFVKRYPENQSMFIIGFGNEGIM
                     DLAGFFQKHTTLDSSLWMIPVSIRSLSRALTPQRTILQLPNRVVLQTINLPERIVYDQ
                     TISERQVDGKQIDFLTFICCGMICDHRFLQNLYKNYPNKKQLTTRPFAGMIEEMIYFY
                     QLEAENLSNYGKLFEQAFYLTENGHLLSASMKKFLGMMFHFVWNLQAYPFDFQLKNFF
                     VWLDLLGYPIHWLEQISKMEYLEKVLFLAEKSKKILVLEKIGIIDGYQHPNEKELLRT
                     MTVFQQIVSEIRGN"
     gene            complement(62370..62645)
                     /locus_tag="U2K71_000361"
     CDS             complement(62370..62645)
                     /locus_tag="U2K71_000361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007208657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HEM7314480.1"
                     /translation="MANKAELIEKVASATDLTKKDATAAVDAVFSTIQDALANGEKVQ
                     LIGFGNFEVRERAARKGRNPQTGEEIEIPASKVPAFKPGKALKDAVK"
     gene            complement(62888..64198)
                     /gene="der"
                     /locus_tag="U2K71_000362"
     CDS             complement(62888..64198)
                     /gene="der"
                     /locus_tag="U2K71_000362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HEM7314481.1"
                     /translation="MANPTIAIVGRPNVGKSTIFNRIAGERISIVEDTPGVTRDRIYA
                     KGEWLGREFSVIDTGGIDLGDEPFMDQIKHQAEIAIEEADVIICVVSGREGVTDADEM
                     VAKILYRSNKPVILAVNKVDNPEMRNDIYEFYSLGLGDPYPVSGSHGLGIGDILDEAV
                     KYFSEETEEEDDDTIKFSLIGRPNVGKSSLINAILGEERVIVSDIEGTTRDAIDTHFV
                     SESGQKFLMIDTAGMRKRGKVYENTEKYSVMRAMRAIDRSDIVLMVLNAEEGIREQDK
                     RVAGYAHEAGRGIIIVVNKWDLVKKETNTMRDFEQEIRDEFRYLDYAPIVFVSAVTKQ
                     RLERLPEMIEQVSMNQNLRISSAVLNDIIMDAVAINPTPTDKGKRLKIFYATQVAVKP
                     PTFVVFVNEEELMHFSYERFLENQIRKAFTFEGTSIRIIPRRRK"
     gene            complement(64380..65609)
                     /gene="rpsA"
                     /locus_tag="U2K71_000363"
     CDS             complement(64380..65609)
                     /gene="rpsA"
                     /locus_tag="U2K71_000363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S1"
                     /protein_id="HEM7314482.1"
                     /translation="MTEFDQNQENNQSMEDAMKSVQEVKVGDVVKGEVLVIEDKQVIV
                     GIEGAGVEGVVPAKELSTLPVEDISEVVKVGDVLDLVVISTIGKDKENGSYLLSKRRL
                     DAKKVWEDIERDFQEGKVIEAPVTNVVKGGLVVDVGVRGFVPASMVEDHFVADFSDYK
                     GKTLSFKIIEIEPSENRLILSHKAVVENEREEKKKEILSTIHEGDVLSGRVARLTDFG
                     AFVDLGGIDGLVHVSEISHSHVAKPSDVLAVNDDVNVKVLSINPEQERVSLSIKDTQP
                     GPWSEIDTKAPVGTVLDGTVKRLTSFGAFVEVFPGVEGLVHISQISHKHIATPHEVLH
                     EGDEVKVKVLEIHPEEHRIALSIKALEEKPASEEEPKNVESYELPEENTGFTMGDILG
                     DALKEEVEEKATDDSEK"
     gene            complement(65744..66424)
                     /locus_tag="U2K71_000364"
     CDS             complement(65744..66424)
                     /locus_tag="U2K71_000364"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HEM7314483.1"
                     /translation="MTQISIAIDGPASSGKSTVAKILAKELNYIYTDTGAMYRAVTYL
                     AITNNVSFSDEEGLVSLIKKYPISFSQQEDGQHVFIASKDVTLEIRQPDVTKAVSEVS
                     AHGKVREELVAVQRKIGERGGVVMDGRDIGTAVLPNAEVKIFLVASVSERAERRFKEN
                     QEKGIPTDLETLTKEIQERDHYDSTRDVSPLKQAEDAVRIDTTGKSIPEVVAAIKAVV
                     LKKGYLLF"
     gene            complement(66473..67108)
                     /locus_tag="U2K71_000365"
     CDS             complement(66473..67108)
                     /locus_tag="U2K71_000365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM7314484.1"
                     /translation="MSRKDRHQSTENNETQEPWEQPIYDTDDEVSSRSSQRQQKKGNT
                     LFLSLFLILLVLCIAIPAGAYFWIRNGSSNNATAATSSETSTSVVESSTSTTKEESTE
                     TSTVESSTIAEESTVSQDTGVTSESSIAAETTPTSSSEATYQAGDSSSNAAETTPSSS
                     AAAGSTTTVQAGEGPKQVAERAGITTDQLFELNGLDPNNFMLYPGQELRIK"
     gene            complement(67174..68577)
                     /locus_tag="U2K71_000366"
     CDS             complement(67174..68577)
                     /locus_tag="U2K71_000366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecQ"
                     /protein_id="HEM7314485.1"
                     /translation="MLINELKKYFGFDSFRPGQEEIIQALLDGEDTLAILPTGTGKSL
                     CYQLTGYLMEGLVIIVSPLLSLMEDQVTQLQKRGEKRVAAFNSLLSQSERRYVLRHLS
                     QYKFLFLSPEMLTNPSLLEHLKKQNIALYVVDEAHCVSQWGVDFRPEYQQLGKIRKLL
                     GNPVTLALTATATDLVARDIRHVLFDRPPKEIRQSVNRQNISLFVRKTQQKEQELEHF
                     MERAQGAAIIYCATKKEVERLYHLFRERFTVGYYHGGLDAAQRRQLQQQFVKNQLQFL
                     IATNAFGMGIDKSDIRYVVHYDLPDSLENYVQEIGRAGRDQEESAAILFYQSGDERIH
                     YFFNQLSREQRQSFELYLEYATEQAPFDELQKKWMELIQQSEKPENWVERLKRQEKEK
                     EFRLQQMLRYINEENCRRKFILAYFGEKLSEKPQNCCDIDGAKAELLPKRKLFSKTEQ
                     LHWGTILLNLFKKNMEH"
     gene            complement(68564..69595)
                     /locus_tag="U2K71_000367"
     CDS             complement(68564..69595)
                     /locus_tag="U2K71_000367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7314486.1"
                     /translation="MIVFILSLFSSGHKLRISSLYQLLVGKRTTSVLIYGFTHELLFI
                     HNSFPELKQDKFYQILQKIAQQGWIEINENEAKLTPAGADMLSEHQIEHTGLRFDRYG
                     RTGETSWRLIKFAVQVISNLATGIQDYLPAETSPFYTFQLKKWLSESQLPREILIDTA
                     YESLVQLFSEIPEEAADFLANQLSGNERTGLLPYQLAKNNDESAVYLQQSRCIHLLLA
                     QIEKRPDSLWHALIDSLLQQNFNQSMMITKQMFMNGQTIDQIMAIRHLKRGTVTDHLI
                     EWALFFDDFPYEWILSAETIERLEPNKDSVREWRFSEWNVDGQLDYGEFRLYQIYLLR
                     KEAIQNVNK"
     gene            69626..69847
                     /locus_tag="U2K71_000368"
     CDS             69626..69847
                     /locus_tag="U2K71_000368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin"
                     /protein_id="HEM7314487.1"
                     /translation="MSLLCKLVPERCIACGLCQIEAPDIFDYDEEGIVLFAQEPEAHQ
                     QFVMPEEETYVKKAYQKCPVRAILLENKI"
     gene            complement(70012..70749)
                     /locus_tag="U2K71_000369"
     CDS             complement(70012..70749)
                     /locus_tag="U2K71_000369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase"
                     /protein_id="HEM7314488.1"
                     /translation="MKIFRRILIGLVIILGLLGGAGYYYIQQNTYEPSSEAQVLVKEG
                     KNKEDYLFFEGEKKNHGKSPLLIFYPGALVEPESYSIWAQDLAKNGFSVAIVKMPLDL
                     AVMGGNKADKVKEDFPNSDYVIGGHSLGGVMASRYVNKNHSDKHLKGVFFMASYPDKK
                     GTLTESAVPVLSITATKDKVLNQEAYQEAKQYLPERTEYVSINGGNHGGFGSYGEQKG
                     DGEAEISNADQQTVIVNTLENWLKKLK"
     gene            complement(70902..72242)
                     /locus_tag="U2K71_000370"
     CDS             complement(70902..72242)
                     /locus_tag="U2K71_000370"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HEM7314489.1"
                     /translation="MKKFVTEQHHEQALESLSELIRIPSVLDEADSGQGHPFGKKVIE
                     ALDKVLEISEKLGFRTFKDPEGYYGYSEIGSGDELFGILCHMDVVPAGDENNWETKPF
                     DPTIKDGWLVGRGSQDDKGPSIAAMYAVKALIDAGVEFKTRIRFIFGTDEENLWRCLE
                     KYNEKEEGITQGFAPDAEFPLIYAEKGLLQAYLTGPGTNEFSVKAGGALNVVPDAAPY
                     SGEKLSEVKEALKKHEFDFEEQGEGIVVLGKSIHAKDAAQGVNAISRLAIALSEVFDF
                     GPINFLGKLVQENATGEAVVGKTEDEQSGELTMNFASLEITPEQTKIGVDMRIPVTFK
                     KDDLVAKLTKTAEKYGLTYEEFDFLDSLYVPLDSELVKNLLGTYRDITGDMTEPFVSG
                     GATFARTMNQCVAFGAMFPDTPDFMHQANERWELSSMYKAMEIYAEAVYRLCAK"
     gene            complement(72479..74002)
                     /locus_tag="U2K71_000371"
     CDS             complement(72479..74002)
                     /locus_tag="U2K71_000371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfcC family protein"
                     /protein_id="HEM7314490.1"
                     /translation="MEQTKKKKFQMPSAYTVLFIIIAVIAVLTWFIPAGHYSVDDAGN
                     IVAGSYERVQQNPQGLWDIFMAPINGMLGVPAGELTKETPAAIPISFFILVVGGFLGI
                     INKTGALDAGIASVVKKFKGKEKMLIPVLMLLFALGGSTYGMAEETIPFYALLIPVMM
                     AVGFDTVVAVAIVLVGSQVGCLASTVNPFATGVASQAVGISMGEGIGLRFLMFVILLA
                     ISIVYVYRYASKIEKDPTKSLVYNQRKEDMKHFDIEAIGRQEVITKKQKHVNVLFFIT
                     FGIMIISLIPWTTLNANFTIFESLTNWLTNLPVLGTVLGKSMLPLGDWYFPEITMLFL
                     VMSIVVAITYGMKESEFVSEFLAGASDLIGVAIVVAVARGIQVVMNNGLITDTILHWG
                     EQGLSSLSSSVFIIITFIFYIPMSFLIPSTSGLAAATMGIMGPMGEFAGVGKDLVITA
                     YQSASGLVNLITPTSAIVMGAVAIARFDVSVWWKFTGKLIAILFVAICVILGVAAMF"
     gene            complement(74380..74991)
                     /locus_tag="U2K71_000372"
     CDS             complement(74380..74991)
                     /locus_tag="U2K71_000372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM7314491.1"
                     /translation="MKNTRVQKMVAVAMFAAIALVLQYFAFPIMPAFSFLKIDFSDIS
                     VLISMFLFGPAAGVATAFLRSVLHLLTTGFSPDNLVGDTASFLSTTIFTFPIYYFFSV
                     KKQHKGRNKFLGVITGTIALTIFMSIANYFVITPLYLMFLGLNATQMLGMSLANYVLI
                     GIVPFNLIKGFIVSAVFLVLHAQLLPWLSRKQHQFEQRHTAVK"
     regulatory      complement(75114..75239)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00050"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="FMN riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="flavin mononucleotide"
                     /db_xref="RFAM:RF00050"
     gene            complement(75321..76037)
                     /locus_tag="U2K71_000373"
     CDS             complement(75321..76037)
                     /locus_tag="U2K71_000373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878594.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEM7314492.1"
                     /translation="MERLQKVIAHAGITSRRKAEEFILAGRVKVNGQVVRELGIKVGK
                     HDTVEVDEVPIYQEEYGYYLLYKPKGVISAVADDKGRKVVTDLLPMVKERIYPVGRLD
                     YDTSGILLLTNDGDFAQRLTHPKHEVDKVYVAKVKGIATKSMLAPLTKGIKIEGRKTS
                     PAKFQILSVDPKTNHSIVELTIHEGRNHQVKNMLQAVGLPVQKLKREKYGDLTLQGLR
                     PGDYRELNKKEVSSLLNHSK"
     gene            complement(76043..76612)
                     /gene="scpB"
                     /locus_tag="U2K71_000374"
     CDS             complement(76043..76612)
                     /gene="scpB"
                     /locus_tag="U2K71_000374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HEM7314493.1"
                     /translation="MTKLSELEALLFIVGEEGISNEELSGLLNLSEAEVTSLVKELKE
                     SYTQNSHSALHIFETESHYVLTTKKELAPLLKRYAQGTSNTLSQAAVETLAIIAYKQP
                     ITRAEIEQIRGVQVSGAVQRLAAHKLIEEKGRVEGPGRPILYGTTGYFLDYFGLKSLE
                     ELPDTQEMEISLEEDVSPDLFFDGMNKGE"
     gene            complement(76596..77393)
                     /locus_tag="U2K71_000375"
     CDS             complement(76596..77393)
                     /locus_tag="U2K71_000375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="segregation/condensation protein A"
                     /protein_id="HEM7314494.1"
                     /translation="MASKEKEERVNELTEINIKLDVFEGPLDLLLHLIQQMEIDIYDI
                     PIATITEQYMNYIHTMKTLELAVAGEYLVMAATLMAIKSKTLLPVQEFVTDDEELWEE
                     DPREELVTQLLEYRKYKYAAERLTEKAEERSLYYTKEPMNIDGLADAEKPLKRGQVKK
                     IDLIRAMQKILERKKFSQKVEKTITPDDTTIEERMVFIEERIKRNAHGCTFDSFFESP
                     SKSEVVTTFMALLELMKKGQIIAQQKENYESIMLYPAVGDTEHDEIK"
     gene            complement(77363..77734)
                     /locus_tag="U2K71_000376"
     CDS             complement(77363..77734)
                     /locus_tag="U2K71_000376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEM7314495.1"
                     /translation="MLTHYRKENRKIAMGLLSFHKKLTGKTSLLKEIDTYESEDDYEL
                     FFFTPEGSTNIQGIIGIYWATNDQLIIHDISLNPSYRGENMGFQMLDELQEKYPDVSI
                     RATEITEPYIGKWLAKKKKKE"
     gene            complement(77753..78640)
                     /gene="xerD"
                     /locus_tag="U2K71_000377"
     CDS             complement(77753..78640)
                     /gene="xerD"
                     /locus_tag="U2K71_000377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific tyrosine recombinase XerD"
                     /protein_id="HEM7314496.1"
                     /translation="MKEQINDYLHYLNIERGLSANTRQSYERDLEQYLHFLQEQKIAS
                     WQDVDRFLVIRFLEKLHEEKKATATVTRMISSLRRFHQFLRQERITDHDPMQHIDTPK
                     KAQKLPSTLTLTEVEKLIEAPDTTKTLGIRDRAILEVMYATGMRVSELIGLKLGDLHL
                     SLGLVQTVGKGDKERIIPLGDYAIQWLERYLDEARPLLVKDASEMHVFVNNHGKGLSR
                     QGIWKNLKQLVREAGIYKEVTPHTLRHSFATHLLENGADLRTVQELLGHADISTTQIY
                     THITKKRMTDVYKQHFPRA"
BASE COUNT        22047 a        16376 c        13361 g        26933 t
ORIGIN      
        1 atacttgcaa agtaatacta caattcctaa cataatcgta gaatttaata atttccatag
       61 cataaagtgt ttagaaaaga acattaagaa tagttctata ataattcttg aactccatgt
      121 cttataatgt tctatcgaag cacccaaata gccaagttca tctaatctac ttgcataaac
      181 tacgtcatcc gctctcatag tcggcatata catattaaag agtaaaaaga taccccaaat
      241 ccctattagt aacaagatca gttctttgtt attcgttatt tttctattat gtttgttcat
      301 ttttatactc cttcttatat ttcctaatag agtataacat agctttaagc attacttaaa
      361 gataacttaa aagttatctt ttaaatactt aattgttaaa taccttattt gatgcaaaag
      421 aaatctacga tagtcaacaa aaaaattttc attatatcca gaaaaatttt tcttagttaa
      481 ggacctatgg attgcagtaa tgcagaattc gtcgaataaa ctgccaaaga catacaatca
      541 tattgctttt aaaattgacg aacaagatat agattcgttt gtctcaaaaa tccaaatgtt
      601 aggtttaact gtcgaaccag gacgttctcg tgtgaaaggc gaggcttctt caatttactt
      661 ttacgactat gataatcacc tcttcgagtt acataccggt actctccagg aacgtttaaa
      721 atcctacaat tccactacct gagactagta atgactagtc tctttttatg gcctctgatc
      781 cccccattga gcgaatacta atagggcttc gaattgatct gttcaatctt taagcaatat
      841 ccctcaaatg agtttggaaa gtttagtgat tcatcacaaa tacttaaata catgtagtta
      901 tatatagatt gaggttcttg tgagcgtatg ctcgtgtggg ttcaaatccc atcgtctgca
      961 tccatctttc caaaacaagc ggctaggaca tttttgtctc agccgcttgt tttctagttt
     1021 attctttcat cttgtcggac cttttttctt gggtatagac ttttctcata ttctaccaca
     1081 attttgggtt tccctaacac atgataggat ctcacaacaa attgctttct attattataa
     1141 taacctgtaa cgactaactt catcccttcg tctacttcag ctaggaagtt caaactatgg
     1201 gaagcaatca aacaattgac cccatttaaa ctgaaacgga tcaacggtct ggcactaact
     1261 tgcaaagtac ggatcttatc tactgttcca tattttttct ccataacctt cacctcaata
     1321 ccattatacg aacatacatt ccctttgtaa agaagaaact ctgttttatc tttttgactt
     1381 tttgtctatt acctttctta aaatcatatt ggcacttcct gcgaagatga aggacatgta
     1441 ctaaaaaaaa gaggcagcag tatagattta agtagttcag ttttggagta caaaaagtga
     1501 aaatgtaaac ctaaatgagt atcaaattgc aactgaattt gatactcgtt ttttattgtt
     1561 aaatcaacgt ttgttgttaa tttacaccac aaaatgtaag ttaaaatgta tataaaaatg
     1621 cgaacgtgaa ataattattg taggaatgca aattggtgtg ttaaaatggt tacaatttac
     1681 taagcaaaaa ggggtgcatt ttcctatgca atttcttgag ccaaaaaata agaacgctaa
     1741 gtctgtggat tgggaaatat cagagcaagt aagggtgata gttaagcaat acgctgaata
     1801 tgcagagcgt actgaaagcg aagcagtgga tgaattctta ttgaacattt tagacgataa
     1861 gaagtttatt gaatggattg ccaacaagcg aagtaataag cggattgttg aaaaaatggg
     1921 tatcaaagat agagtgggtt gatagacaat gaccaagctt aaaagattag ccacgaagga
     1981 agatgaaatt gtagatgtta aaattccgat ttcaaacgaa gaattaaaag atagggctaa
     2041 acaatatgat ttattaactc caaaacgatt tgcaacaagg tataacaaaa tgttgttctt
     2101 gccaacgtct tttaaatgga acggttctga atatcccatt caatataatt actgcatcaa
     2161 tccgttttgt tgtaattttg ggaaagaaca gcacaaattc aaagatgtaa aagggaaacc
     2221 aagtcgatat aaaatgactg gtagttccaa agataaaggt cataaaggta tgtactgcaa
     2281 tgacaatcct attggcagag gtgtatcgca aaactgtaca gtaacccctt tatctaattg
     2341 gtctgttgtt gaagaaatta aacgccttat agaaatcaat agcatccaag atgttgagcc
     2401 agattatcaa tttcataaag aaggctgctc ggaagaagaa tcaaccccat tcaatgagcc
     2461 gaagcaattt tacaaaaggg ggaaaagtag gggcaaatct caacgctatc aatgtaaggc
     2521 gtgtaagaag ttcactaatg ttctaccaaa aagagaagaa accactactt atcaccaaca
     2581 aaagaacacc attcttccaa tgttcgctaa gatggttgtt ggaagggttt cagtcagtcg
     2641 tacttgtgat attttaggaa ttggagttgg aacctactat cataagctgg agtggctgta
     2701 taggcgttgt ttggagtttt tagaacgata tgagacacaa ccccttcaaa caaagaaatt
     2761 taacgagatg tggcttaata cggacaagat gcactactac ctaaataacg tccgtaagaa
     2821 aggacaaggt tcaaagaaat atacggggtt tgaagacctt aatatgcaaa cgtatatagt
     2881 tgtatctgct gaggtcttat caagatatgt gttccgttct gatgttgcct atgattggaa
     2941 tatttcaatg gatgaattaa atgaagatac aagaaaattc aaagaagacc atctcaatac
     3001 ttttagtcgt aaaaatgata ggttagattg gtcttattat ccacaagaac catccgctaa
     3061 cgatagcgaa aataggaatg cttatcttca cgaattaggg aaaattacta ataggagtag
     3121 atttgtcgat ggtttaaatg ttgatgctcc ttatactaca actgctcact attggttaat
     3181 taaacaaatg gttaatgccg acgaatggcg aatgattagt gatgatgact tctctattag
     3241 aaacgccttt tatagagtat tcacgaaaga attaaggttg agcgatgcac atcattttat
     3301 ttgccaagtt aacaagacga aaagcagaaa acaatgctta aaagaattcg gacaagcaaa
     3361 agcagaactg ctcgattggg gagatatcag aggattcaaa acaaagtttc tacggacttt
     3421 ggcatctcat tatctaactg aattactaac aagtcatcaa ttccacgaag aggctattag
     3481 taaagatgga gaaagatatc gcaaatatgc cgataaccct atcaaacatc cattagccac
     3541 taaggataaa ggattttatt cagttgattg tagaacggat ttatctgctc tggaaccaaa
     3601 cgaaatcgca aagatgctat taaatgttaa tgaccattcg accaatagct ttattcaaca
     3661 gataagacgt tatatatcat ctcttgaaag accattaaca acagctcgtg gtgataagaa
     3721 gagctatatt tacgctaact tcaatccgaa gtatgctcaa tttgcgatta caattctaag
     3781 aacttactac aacttttgtc gcccttttaa atcagcggat aaaaaggttt tgacacctgc
     3841 tcaaagatta gggattacag ataaacaatt tgattggaaa gatataattt atttcaaatg
     3901 acaaaaagag cttggagtaa tcccaagctc ttgtgcttta tctttactgt tcttcttcaa
     3961 ttaacgcacc cgttaactca ataagttttt tctttcatat acatgaaaat agtagttata
     4021 cggattttta tcattcttta tccctttttg ggcaaatacc tcattccatt cctcataatt
     4081 cacttctgga aaaaaagtat ctccttcgaa ttcatgatgt atttttgtga tgtacatttt
     4141 ctcaacataa gggaaaaaca aattataaat ctgttctcct ccgaaaataa aaatttcttc
     4201 ttcgttttta cataactcaa aaacatcttc tattgaatga acaatttcac aaccattaaa
     4261 ggtaaacccc ttatctctcg tcagaataat atttcttctg tcaggtaagg ctcttccgat
     4321 tgattcaagg ttcttcctac ctaatattat cggatgtccc tttgtagtat ttttaacata
     4381 ttcccagtcc ttgggaatcc tccaaggaat gtcattctct ttgccaatca ctctattctt
     4441 atccatcgca gcaatcaaag aaactttcat ttataacaca gcctttttaa ctaatttacc
     4501 atctaatttc ttatttctat caaaaaacct tttctcatta tcctgcccgt tagttgaata
     4561 agcaattttt tctataagtt atattatcat aataccacaa aatcacaaat tttcattcaa
     4621 ttatcatttt caattgcatt ttataaatca ttttgtatgt aatttggtat gtaagttaaa
     4681 atgaaaaaag tagtccattt tgaagtacat tttgtcattt tgtactccaa aactgaacta
     4741 cttagtatag attactgttg cttctttact aatttaggtt tacttgttaa gaaagattat
     4801 ttacgcaaat gttcttcaac aatttgctga gatttatcag ctgtatcttc cacttcatct
     4861 tttgcttttt ctaacttttg aatatattgt ttcaagcttg agttttcagc agttgcttcg
     4921 tctaatttct tttgtgtatc tgttaactga gtttctacat ttgttttttc ttgtgtcagt
     4981 gttgcgattt gttgattcaa accatttaca gtattttgta attctgaaag ttttgcttgt
     5041 gcttcttgta gtcttgctgt ccaatctgca ttcagatgat tgatttcatc atttttttgg
     5101 ttgatctcat ctagtttgac attgatttca ttttgtttgc tttgtagttg tgcatccttg
     5161 ctgactaatt gactttctaa gttttgtttt tctgctgccg attgattttt ttggttttct
     5221 aattcattta cttttgtttg gtattcttgg acatctgcct ttgaatcttg caatgtctgt
     5281 tctttttgtg ctagtcgagc ggtaagatct gtaatcgctt gctctttatc tgtcaatgta
     5341 tgatcaagct gtgtcagatt ctcattgatt gtatcaatat ctgtctctcc accccacaaa
     5401 ataatttcat ttgcgtaccg cgtgcctgtg tagccaatta gacatagaaa tgcaacgaca
     5461 atcccaccag taataaattt ctttttcatt tgcatagctc cttaatccaa tacttttaca
     5521 tgctaaaagt aagtgatctc aaacacaatg tcaagcgttt ttaaattaca tatgtaattt
     5581 taatataaat actatcaaat actcatttaa tcctcctttt ttatctattt aaaaaatctt
     5641 ttttatagag aaaaatgcag gttaatactt tcttaaataa atatttatgg tgaacaaaaa
     5701 caaaaaagca agttttatta aacgtaaccc ttttcatttc atagattact gtatttatta
     5761 caaaaaacgc ggatttgtca taaattgtca tgcttctttt acctatgaaa tattttcacg
     5821 tgttaaagta ttcctcgttg aacacaacaa ataaaacttt ttaacgaata aaggagattt
     5881 ttcatgacat cacttaaaac tttacttttt ggaacaactt tggctgccgg cgctgcattc
     5941 ttcatgggaa cgactgctca tgcggacgaa gcttataccg ttcaatcagg tgatactcta
     6001 tcaacgattt ctcaaaaata cgttggtgat aactccttga tcaatgccat tgcagaatca
     6061 aattcaattt ctgatattaa cctgatttac tcaggacaac aattaacaat cccaacagaa
     6121 ggctctgcac aagcagctgc tgaacctcaa gcagccgttc aagaagcacc agtccaagct
     6181 gaacaacctg ttgtacaaga aacggttcaa acggaaaaac aagcagcgcc tgttgctgaa
     6241 acacaacctg ctcctgctgt aacagaaact gctgctactc cagcaagcac aagctctgca
     6301 aaagaatgga tcgcacaaaa agaatcaagc ggttcttaca cagcaacgaa cggacgttac
     6361 atcggacgtt accaattaga ttcttcttac ttaaatggtg actactctgc tgcaaaccaa
     6421 gaaagagtag cagaacaata tgttacttca cgttatggtt cttgggaagc ggcgaaagca
     6481 ttctgggaag caaacggttg gtactaagat tcatttatta atcaaacagt ttacttttat
     6541 acaaagaaag ccaaaaagag gtgcgacact agttgtgtca catctctttt ttatttacct
     6601 aatctaatga aacgttccaa gttttgataa gtattttttt taacttcttc aactgatagc
     6661 tctttgatct cagcgattgt ctttaactgt tcttcaagca gtgagacata atctttttct
     6721 gaaaaaatcc cccctaaagc ccactctgct gcatgtagtc catccgtttc aataagtaag
     6781 tggtcgattg ggactgcttt agctagttgc cagactgctg gattcttttt caaatcaaga
     6841 ccaatgctga aataacagcc taaatcaata tagtcttgtt gatagtccaa agaagaatac
     6901 caatggatca aatagtgatt agggtaagct tggatataat ttaagatctc tttttcgcat
     6961 ccttttgtgt gcagtacaac aggtttctca tttatggctg caaaagcaag ctgtcgctca
     7021 aatacttttt tttgtgtagt catcggtaca ttcgtccaaa tattatctaa ccctatttca
     7081 ccgatgatcc ttgctttttt caaaaaaggc tcaacttgtt caaacgtata gctatctgct
     7141 ttccaaggat ggataccgta acttaaggct tgagtcttac ttgctgcaag ctgtctgttt
     7201 tccttccatt cttctggcga atcacaattg atgatcgtca aaatcgtgtg ttcctgctga
     7261 aggagtgcta aactctgact cccatctaaa tgacaatgag catcacttaa catctaattc
     7321 acctgccaat aactgataaa tcgtttgctt caactctgat tgctgcagta gccattcacg
     7381 tgaccgtcgt ttttcaggca cttgaatctc agcaatcaca tgtgccggac gggcggaaag
     7441 cacaagaatc ctgtctgata aataaattgc ttcttcgata tcatgcgtca caagtaaagt
     7501 agtccgcttt gcttttttag gcagcttcaa catccagtct tgcatctgcc ctctggtcat
     7561 agcatctaac gctccaaatg gttcatcaag aagcaagtaa gaagaaggat tcatgattgt
     7621 tcgtaacaag gccgttcgct gcttcatccc gccagataat tgttccggca aaaaatgttc
     7681 atagccgttt aacccaaaga tatccagata atcccttact tgttcttcat tgattgttag
     7741 atgattgatt ttttgatata acgtaacgtt ttgatagacg ctcagccaag gaagtaacat
     7801 atcttcttgc ggcatataac taaatgctgt atggagacca gttacttttt gatcgtctac
     7861 tttgatcgtt ccagaagtca taggctggac tcctgttaaa gttttcagca atgttgactt
     7921 accggctccg cttggcgcga caatcgatgt aaaggtatct ttagggatag tgaaatggat
     7981 gtcttctaat attttcaggt tttcaatgac tacattcaag tggctgattt ggattgacat
     8041 atctatcact cctattttat aaactgatta gtgaatagtt cttgcgaagg tacatcttgc
     8101 ttgatcaatc cgttttcctg cataaaagct gtataattgc gccaaacttt ttcttccatc
     8161 aatccccaat tctcaggatc atccgtataa ttttttgaca agaatgcttg tgattcttct
     8221 aaaaacgtcc gatcattttc ttttgcatat ttttgcaaca gtttagcact gtcatttgga
     8281 tccttaatag cttcctgata gccttttttc gtggcatcca taaaagactg taccaattct
     8341 ggatcacttt tgatcagttg atcattcgta ataattaccg gagtatagta atctaaccgt
     8401 tcatctaatt ctttcaatgg gatatagttt agttcaatac ccgcttcttt tgctttgatc
     8461 atatcccaac cttcaaagta ccattggata tctgaggatt ttcctaagct ttctggccct
     8521 tctccattgc ttcctaccat tgtcagtttt gaaaagtccc cgccgtcttt ttccatcaca
     8581 gcttttaaga cagcttcttc acttggactt tgccaacctg catatgtttt tccttcaaaa
     8641 tcttttggtg aatggatgtt actgtctgat agggtggcga atccagaagt attatgtttg
     8701 atcactgtag caattgcttt gactggtata ttctgtccat ctgcgtgagc ataagtgaca
     8761 tcttcttgat aacttacacc aaattgtgct ttgtccgcac ctactagacc aatacttgtc
     8821 gaattatctc ctggttcgat gatttggaca tttaaaccag cctctttata atagcctttt
     8881 tcttttgcta ggtagatgcc agtatgatta gtattcggta cataatccaa aacaagtgtc
     8941 acttttttca aatcctttat ttcttgtttg ctacttgttt cttttgcagt gccacaagcg
     9001 gataaaagca acagtagcgg taataaaagc cataattttt tcatttgatc cccttctctt
     9061 aaaaaatata aagcatacga taataccctt ttttcaataa tgaacaagcg ccattcagca
     9121 ataaactcaa tagaatgaca caaatgatcg cagcaaaaac tttatcgatc tggtagctgt
     9181 tttttacgcg tatcatgtaa taccctaagc ctgcttgagc gcttagccat tctccaacga
     9241 ctgctccgct gacacaataa gtagcagcca cttttaaacc ggaaaagaag ccggataaac
     9301 ccgctggtat tttatatatt cgataaatct tccctgtatc tgcccccatc gttttcaaaa
     9361 agatgatttg ctcttttgat actttcctca atgtatccga aaaagaaact acgatcggaa
     9421 aatagcttat caagacaaca aggatcactt ttggaagcat accgaaacca aaccataggg
     9481 taagtaatgg ccctaacacc atgattggca acatttgtga aatcactagt aacggataaa
     9541 aagacatttc taaaaacttc caataatcca ttgccaaggc agtaataaaa gaaagaacgc
     9601 tagctattac caaaccaagc actgcttcat ttaatgtcgt gacagaatgg gaaagtaata
     9661 cttgtgcatc tgccgctaat gcagaaaata cagcagcagg tccgggtaaa ataaaggcag
     9721 gtattttcca aactatgaca acaatctgcc aaataattaa aatgaatgcc atactgactg
     9781 ctggataaag atttttcttc attttgttac ctcctttctt ctaatatgaa taacaaaaaa
     9841 cgcttgaaat gggcacgaca tagtaacccg cttcaagcgt ctgctatgtt tctcccttca
     9901 ccagtattaa ctgacaggtt caatgggtct aagttcacaa cttactctca tgtcgtctac
     9961 gacatcccca gaaacaggta ttcatttttt aaagtgtagc acaagaagga aaaagcaaca
    10021 agcgctttag ttatcatgct gattatttca ataaatagga gagatcactt gccaatgtcg
    10081 gataccccat gatatagcta tccaactctt ttttagtcaa tttttgattg atgacgagtg
    10141 tcaaatcatc gatcaattcg tctgcttgct cgctaatcac tgcagcacca atcaaataat
    10201 tttgctggtc aaaggtcaat tcagccttag caactggttc attgacacga tgataggtga
    10261 accaactggt aagatccatc tctaccactt gatctgacga agaggcgtga ctttttgtaa
    10321 ctcctacttc agcaagttta ggactggcgt agacgatcgt aggtataatc ggatatttga
    10381 taggttcgct tgttgcatcc gttatccgct tagctacata atttccttca aaagtggcga
    10441 ctggtgtcaa ttttggggtt ttccttgaaa caatatctcc acacgcaaaa atttttggat
    10501 tgctcgtttg cagataatca ttgactgcaa ttccgtgttt atcaaagacg atattcgctt
    10561 gctcaagtgc taaagattct gtattcggct gtctcccagt cgcacaaaaa atcatatctg
    10621 ctactaattc cgtctctttt ccgactaaac gatagcgagt accttctgaa atgatctttt
    10681 gggtttcaac accaaaagca aactgaatgc cagatgcttc catttgatgg acggcttcct
    10741 ctaccaaaga cgcctcaaat tcttttaatg gccgttgatt atgatggaca atggttactt
    10801 tgctccctgc agcattggca atcgtagcta gctcaaaagc aatataacca ccaccgataa
    10861 aaatgatttc tttaggcagt acaggcaatg ataaaaagtc tgcactagtt ttcaagtatt
    10921 cctttccctc cactggtaag atcgtaggtc tttgcccggt tgccaaaact aagtaatccg
    10981 catggaagac ttcttcgttg acttcaatag atgaatcatc caaaaaacga gcggttcctg
    11041 aaagatgatc gatttcagct tctgctaatt gtttttttcg gctttctggg actggatccg
    11101 taaatgttcg tttgaatttt tgaagttctt cccagttggc agttgggatc tcgttaaaac
    11161 cttttccact caattgtttt acccgattac gtgcctctac tgcacttaac aagacttttt
    11221 taggatcaca tccacggtta ggacaagtcc cgccccatag atcttcttcg accactagta
    11281 ctgttttacc tgcttctttc aatccataag cagcacttaa gccgctcaca cctgaaccga
    11341 tgatgattgc atcatatgtt ttcatttcct tcctcctctc gacttaaaaa agcaaagatt
    11401 tctttacttg tcagcgaccc aatacttctt tgcttttcta ctatcacaat gcttggatac
    11461 accgaaagaa gcgaatatac ttctttcata ggcgtatcaa agcaaacttg ttgacgagca
    11521 ttttctggta tttcatttaa gaaatatcca ttgacgacta aatcttctac tcgccactct
    11581 ttcgtctgtt tcggttcatc aaagaaatgc cgaacgtagt cgtttttagg attcatcaat
    11641 aactgctctg gtgtgtcaca ttggataatt tctccatcct tcataaatgc aatacgatca
    11701 cctagcttga ttgcttcttc catattgtgc gtaacgaaaa cgatggtcgt gcccaattct
    11761 tcgtgtaagg acaaaaccaa ttcttgtaat gagtttcttg aaatcggatc taatgcgcta
    11821 aatggctcgt ccatcaaaat cacatctggg cttgcagcaa tagctcggat gatcccgatc
    11881 cgttgctgtt ctcctccaga caactctcta ggcatacgat ctcgataaat atcaggagat
    11941 aagccaactt tttgaagaag ttcatctaca cggtctgctc ttttctgctt ttcccaacct
    12001 aacatttcag ggatgacttc aatattttgc ttgactgtca tggtcgggaa caaagcgatt
    12061 tgctgcagca catatcccat attccaccgc attttctgca gattatattc atttaacgat
    12121 tttcctttga agtagatgtc tcctgctgat ggtttagata atccattgat cattttcaaa
    12181 gtagttgttt ttccactacc ggaaggacca accaaaacaa agatttctcc ttttttgatt
    12241 gccagattta ggtcatcgat gacatacttt ccatcatact ttttttgcac atgtttgaac
    12301 tcgattaatt tagtcactga tcttcctcct ttactaatcc ttcatttttc aggaagattc
    12361 gtgctacttc tgtcggcttt ttcttttcga cattgacttg gtaattcata tcaatcattt
    12421 gatcctctgt tatttttccg cttaatttat tcagtgcttt aacgatttca ggatgttctt
    12481 tagcaaaatc ttttttcatc agcggtgcgc cttgataagc tgggaataac cctcgatcat
    12541 cttctagagt cactagatcg tactccttga gctggctatc tgtggaatag gcatccacaa
    12601 tattgacatc tccattattg attgcttggt aacgcaaact tggctccatg ctttgtacac
    12661 ttgaaaaatt caagccatat gcttcttgga ttcctttata tccatctgaa cgatcaataa
    12721 attccagtgt gaatcccgct ttgatctgat cagagatagc tgccaaatcg gaaatctttt
    12781 taagttgatg ttcgttcgca aaagatctct tgactgccaa ggcatacgta ttttggtaag
    12841 ccattggttt taataatgtc atatcgaatt gcttattcaa taattcttct gcttcgtcat
    12901 atgttttttc ttttgtcaaa tcagcacttt tcattgcttc aggtaccttc accaagcttt
    12961 ctaggacagt acctgtgaac tcaggataga tatcgatttg accttgatcc agtgcattga
    13021 acaaaaaact cgtcttgcca aaattcggtt ttaattcaac ttctgttttt gtgttgtctt
    13081 caatcaattc tttatacatt tggatcagga tttcaggttc cgctcctaat tttccagcaa
    13141 tcgtgatggt ttcattttta aacaaggcag actgatataa agaaacgccg ccaaataaac
    13201 acaccactgc tgctaaagta atgatggaat atcgaggttt cttatgctgc aaccagcgaa
    13261 tcagtccgct gaatataatt gcaagcaagg cagaactgat cgcaccaatc aatgtcaaaa
    13321 caggtgtatt tcggtcaata cctaacaaaa taaatgtccc taatccacct gctccgatca
    13381 atgctgccaa agtagccgta ccgatgatta aaactaaggc agttcggata cctgagataa
    13441 tgacaggtaa tgcgatggga agctctactt tcagcaagcg tctcattctg gacataccaa
    13501 atgctaccgc tgcttcttca atagacgggt caatttcagc tagaccaata tacgtgtttt
    13561 ggaaaatcgg aagcaaagcg taaataacaa gtgcaatcaa tgctggaaca gttccgatcc
    13621 ctacaaatgg gataagtaaa cctaataagg caagtgaagg aatcgtttgg agcacacccg
    13681 tgatctgcaa taagacttct gccatttttt tatgattggc tgcccaaatc gctaaaggaa
    13741 tagcaatcag tgctgcaatc agcaaagaaa tgagagaaat ggacagatgt tgaaatgcag
    13801 cttccagcaa ctcgcctttt ctttcactta atgtttgtat aaaattattc atgcgacctt
    13861 cctttctgtt attctttaag tataaacatt tctttccttt ttcccaacta acttgtcttg
    13921 ccttaacctt acagcacgaa caaaaaaaag aaaaaaatac ctctaaatat gctgacaaaa
    13981 aaagaatcca agcaacaaag tcggatcaac tttttggttc tcgttttttc actacgaata
    14041 ccataaaaat ctatacgaaa ttgtcacttg gaatcagcta tatctttttt acacacacaa
    14101 cttttatttt aataaaaaga aacagccaat aaagtagaaa tctcgtgggg gagaacacct
    14161 ttattggcct gttcaacgaa tattatactg tatcatgaca cttttttgcc aaaaatgtaa
    14221 aaaatggttg ttttgacatc ataaacgtct tgttttctta tttttactta tatcaccact
    14281 ttattttcta tctctttcac tccataaaga ggaatgagtt cttcttttga ctgataataa
    14341 acagctttct cacggttctt catcaaaaaa agaaagatgt ctttcatagc aagtggtgct
    14401 ttgccgtgtt caaataaaaa gataaattga tttgctttat ttatctggac cattgtcaca
    14461 ggcaccagtt tttgttcttg agccaaaaaa actccttgat actctgtttt tttgggtgat
    14521 tttgtaagaa ttgtcaataa gtctgctgct cgcatttttc cactccttat ttgataaata
    14581 aaaagaaaca gccaaaaata attttttttc attttcctct tgaagtaaga gtataagtca
    14641 gtataataaa gaagttgctg aaaattttca aaagaaaaga ggctagaaat gaaaaaatta
    14701 aatttattta cagaattgtg cggaatcatt ttaggcgcag gaatctacgg attagctgtg
    14761 acaggaatca atattccatc taagctggca gatggtgggg ttactggtat tgctttgctt
    14821 ttgcataatc tttttggatt cgctccttct gtcacttcac tgctttttaa tttaccttta
    14881 ttgcttatct cattgtttct ttttggaaaa cacgcttttc tccggacgat cgtcggaaca
    14941 tttgcgctag ttttcttttt acatttatgg gagagccttc atatacattt tgctgtagat
    15001 agtctactga tcaatagttt gatgacaggt attctttctg gcattggttg cggtctcgtc
    15061 tttcgattcg gcggaagcac aggcggaaca gatatcattt atcaagccat tgaaaaatat
    15121 tttcatatta agattggcaa aagtcttttc ttgattactt ttggtatttt agttgtctcc
    15181 ttgctttatt tggatctcac acattttctg tatacattac ttagctgttc tattctatct
    15241 tatacattga ataaagtgaa gtatctggaa attggatctg ttttgcccca attggaagca
    15301 aaacagacta cagaaacatt tgaaccactt gatcctcttg aacagttaga gcactgattc
    15361 ttatttttca ctactttatt taatttacag aaatatcatt atgtaaacta aaatgatatt
    15421 attcagtaaa atcttcttta taaaaaaata aactattttt gcaaacgatc tgcgaaaata
    15481 gtttgttttt ttattttgcc attattttct tacttattgg gagaaaaaat tcgcttaaac
    15541 tcaaaaaaat ttgaggatac gatataaact ttcggataga gtataaaata aggaaacgcc
    15601 gcagcgtttc ctttccaact gtagccgaat ataaatttgt atatcactta tttaaaatag
    15661 ctattttctc ccaaagagtt tccttgatca tcgatcaatc cttgtaatga gcgttctaag
    15721 ataccatggc tttttttcga ttttttttct tctgactgct ttgtttcctc taacgttttt
    15781 gttcgattga atgttcgtac agacggctca ccatttcttt tttcttgata cggcgtcggt
    15841 tctgtatcga acaacagata agagtcatcc gtttttttca tcgattcgat taaatgatcc
    15901 tcagaaattt tgctttctct agggtctggg ataatcgatg cgggtacata tttaggaaca
    15961 aaataagaac gctcccgcac cttcgtgtca acagatgcgt ttcgtgtagt atgcttggac
    16021 tctgtccttg tttcactttg tctgacagaa cgagaggaat caccaaaaag tttttttcgt
    16081 cctgtcgatg tgatttctga ttggtaatgg ccggtataat ccggtaattt tcctttatgc
    16141 ttttccaaat tttccttacg cggagctgac ttcaatccat ttgttgcacg acgcttgcga
    16201 cggattttct cggtgttcgt gttcgtgaaa tgaaattctt tcatctgttc aaacggggac
    16261 tgattctgtt cagtcaaaag atcttcttgt ccgtcaaata atactcgctt cttgtcgttg
    16321 gttaatttta cgcctaactc atcatcgtac gcaggaaaac ggaaatgttt tcgtctgatt
    16381 tctttcattc aaaccatcct tctcaaaaat ggaacttttc tcaataatag cacagattca
    16441 ttgttttcag tagcaatgcg ctgattattt taagaaaata gcgatgaatt tcataaatat
    16501 atatatagta ataagactta attcgttgtc tctctgataa ctagttcagg aaaaaaacga
    16561 cgataacctt gactcgcccc tttttcttct atttttttta acaacatttc tccacatact
    16621 tcacccatac gtataatatc ttgcttgatc gttgtcaatt taggtgaact gatttggtct
    16681 aaaaataccc catcaaatcc aatcacacca aattcttcgg gcaagtgtcc tccagcattt
    16741 ataatccctc ttactgctcc tagtgctagc cgatctgaac tacatacaaa acaagtttta
    16801 tctgtaaaat ctgcccagtt ttctgtcata tatctttcta cttctgtcga atgatttgga
    16861 aaccgcaaga ttttactagg aagatcaact tccttcatca cagataaata accagtttcc
    16921 cttgaaattt caaaaggctc gtccacttcg ataccgatat aaataatcgt ttgataatct
    16981 ctttctaagg catattgcgt tgcttttgct gtaccatatt cattatcgct gtctacataa
    17041 tcatatcctt cagaattttc cccaaacaag acaactggtt tttccaaacg gtcgatccaa
    17101 tcgaagtctt tttttctgac acctgtgatg atgtatccat cacaagaacc gacgtcgaag
    17161 gcatcatttg taactaattg gagagaataa tgcgctttac cgatccgttt agcgattccc
    17221 atcagcaaat tcatgtaata gggctcagtt gtgtcgattt cttccagtat aaataatttg
    17281 atgatctgcg accgattcgt gacaagtgct tttgcagcaa tatttggacg gtaatccagt
    17341 tcagccattg ctgagaaaac taactctttc aattcgtcag tcacctgttc cggatgattg
    17401 atgactcgag agaccgtcat ttttgaaaca ttggcacgtt ttgcaacatc tgctaaagtg
    17461 ggcatgctct cttcctttct aacatgttgt cattcttact atcaattata ccaaattttc
    17521 ctaacagagt ttattatagc ccaaaataaa aaacggaaaa tcctctatcg tcatgagtga
    17581 ttttccgttt caattattta tactttttta tcaaattttt tgctaaaaaa gtggaagctt
    17641 cttccattag tggtatttgt accatatgtc gctgtgtttt tgaggaacga aaaacaattt
    17701 cttttccgta agacttatgc cgattttttt ctacaaatcg ttccgtttgt atataaggga
    17761 ttttttcgtc agcttgtcca tgccaaaaga acagaggacg tccagctaac ttttccggct
    17821 gcaatgacaa atcatagctc ttaacccatg aaatcaaatc aaaatagtct tttggtacga
    17881 cataattata ttccgatgcg cgccttacga tttcttttcc atattccaaa ggtgcagggg
    17941 aacccatcaa acacacgccc gctactatat gaggatgttt agccaacaag gcgcatgttg
    18001 tcattccccc catcgaaacc ccgccaactg caagctgatc atgtagaaga cctctttttt
    18061 ctaaatgctt gataagtgtg tcaaattcaa acagatttcc gtagatacta tcccaaaatg
    18121 tataggatgg aatagaagaa accggctgtt tgcgctcccc atgattcatc gcatctggca
    18181 ggatcacacg aatgccctgc tgcgcaattt ttcttccttg cgtcaaaact aactccttac
    18241 ttgtctgcca tccgtgataa tagatgacaa cgggaatacg ttcctctctt aattctttca
    18301 gaacaacttc taacactgga atatgatgga cagaacgttt caatataatt ggattcaatt
    18361 gttactcacc tattttcatt aaatttcctg atcgccatag caaccatgct ccaacagttg
    18421 tctggaacag gttcagtgta ccaaataata taaatgcagg actgaatggc tcgggaaata
    18481 agcacagaga acaaagtccc accactccta atgctagcga aaggagtgga taaatagaaa
    18541 aattcaaaaa actggcaacg ctaccgaaaa tattaccaat aaacagtatc aaaaagaaaa
    18601 aaacgcttaa aattaaacca tctgtcagcg acatctgaaa aacaatgttc tctacctcaa
    18661 ttggctgttg aatcgaatag acacctaagt atatactgag aaggagactt agcacgtggc
    18721 tggcacaaaa cacacgattg accgttttca ttttaatcac ctaacctatc tttataattc
    18781 tattataatc attttatcat ttttttgtat gtataaagtt tcaatttttc ttcacttacg
    18841 attttttttg atattgttgt tatataaatg aaaggagttt tacaaatgat tgatccaaaa
    18901 atgttgggga tgatcttcct cattaatttt gcttatatca ctctgaacac gttacgtttc
    18961 atgttgacaa tgaaaggcta tcgactgatt gctccacttg tcagtatggt cgagatcacg
    19021 atttatatcg tcggcttatc ccttgtttta gatcgtttag ataatccact gaatttattt
    19081 gtctatgcgt taggttacgc agtaggtatc agtgtcggaa ttttgattga agacaaattg
    19141 gcattaggat acatcatggt cacaacgatt ttgccagcaa atagcgcaga agataaaagt
    19201 ttgccgggta tactgagaga acacggttat ggtgtgacac agacttctgc aatggggctg
    19261 gaaggtgagc ggctagttct cgaaatactt tctcctagaa aaagcgaacg tgaactgtat
    19321 cgcctgatca aagaaatcga agagcgtgct tttatcattt cttatgagcc aaaatatatt
    19381 tcaggtggtt tctggactaa aaaagtgaga aaacgacaag gaaaataact cacaaactta
    19441 aaaactgtct aatatctaaa ttaaatttta aatgttagac agtttttatt tattattttg
    19501 attaaaaaac atcgacatat actcatcaat ttgttattat actttatata cttatcaatt
    19561 taatttggag atattatgga aattcaacgt agaaccacta attttttaca tttaggctct
    19621 ttttttgcaa ctttgacgat cttatttttt ctatgttatt cataccttaa gacctcacat
    19681 gcgccttcta gtgcattagg ctggttgctt ttggatcatt ctgatatcac agaagccgaa
    19741 gaaaatccgt tttttttgat tttgtcttct ctttgcggct tactgttttc tgtttatttc
    19801 ggtgcctatt tttttcatcg acatcctggt acgttaaacg aagcttcaag cgtattgatt
    19861 gcatttaaaa cgttttcttt tttagcaatt cttttttatt cgtttaatca caatacgctt
    19921 ttcttcttta tcatgaatgg tttagtcgtg attttatcca tttttactta ttttattttt
    19981 tcactcattt ttagccaatt gaaatgtccg ctcttctttc gattagttcc tgtcacctgt
    20041 tttatttaca gcttgttttc cttttttgtc cttgctttaa tgccactgac caacccttcg
    20101 acgatccaag tcgcaggaaa tctgttggat atgctcttca tcgtcagtct aggtgtattt
    20161 atgtggcaaa gaaaaaacaa acaaaataaa aagcattttg aagcctgact attacgactt
    20221 cataaatgct tttttagttt taaacacttc aatctaaaag aataacccaa gattggataa
    20281 attattaatg actcgccatt cgtttcttag tgattctgga ttatcaaatg gacttcctaa
    20341 tctaatcagc tagttcttcc cataaaatgc catacttgac tgaatcataa tattgattat
    20401 ttaaaaatct aacttttctt atcactactt cctttgtcat tccacatttc tcaccatttt
    20461 tttgcattcc tttgtttcca gaccatgtag tgaatctaat aggtggtaga taggcaaaat
    20521 actcaaataa ttcttttaac cataaaggaa gagaaaggct cctcctgcaa gagtcagtat
    20581 aatttttgct tactgctctc ttaggtttta ctcactaaaa ctgttccata ttctttgata
    20641 taagaaaatg catgcggtat aaatttcttg ctcagctcag aaaagatttg tcgtttaaga
    20701 aaggccattt cctccattgt caatgaagga tccaatgttg gtaattcaat ctcaaaattg
    20761 tttttttcga caataggtaa ttctaatgtg aatgattctt cttcctttat agtattcgtc
    20821 acaagtagag actgatggaa aatactaggc cggcgatttt tatatttatt cttacgtatc
    20881 ctgtcaacga taacttctat ttgatcaaaa aagtacgatt catttacgtc tccaataagt
    20941 tcggctaatt ctttgaatgc cttcgttaca ggaagctgat tcttctgtac aaaaaagtaa
    21001 ttagccaaag taagcgggaa gtgttctata gaggaaagtg tgataaaacg gtacttctca
    21061 tttcctgcag ttgtcctcag caattctgtc tctcggctgt ttcttactcc ataaaaatac
    21121 gcttctttcc gttcatcttc gaaacaatac gcccaaataa cttctcccat cgctaatttt
    21181 tgttcatcaa cagagagtct cttcaattgt tcagctatgg tttcagcagc cgaagtggct
    21241 tgttgcaagt aatctttagg atcaaatatt ggaaagttca aatgatatct tcgttcttcc
    21301 ctcttgatta atcctgcatc gatcaataaa tcaagaaaat gggaaaattt attttgcgaa
    21361 aattcttttt tcaattctct tagaatcgtt tcttgttctc gatgttcata tagataatcg
    21421 gtcaatgaag aaaaaagcgg atcatcggtc agtttttgat aaaagttttc cttatttttt
    21481 gaataatagt tcatatatgt tctcttttct atccgtttta ttttacttaa aaggctttta
    21541 cgtaaaaagg aggaagaaca agagtcataa ttgactgtta ttctcctcca tttcttcggc
    21601 ctttagtatg ttctatgttt ctttttatat caatcgctta tccattcatt ttgaatgctt
    21661 catactacca tttgattagc taaatacagc cgtcaattga ggtacgactt gttttttacg
    21721 tgaaacgaca cctttaagca aagcacggtt gttggtcaaa gttgtttgga aagcttcttc
    21781 tactttttct tttcctttac cagcaacaag taattctgaa tcactgtcta agatgtttgt
    21841 gacaactaat acgaacagat cgtagccatt tgctttcatt tcatcttcca ttgctgcttc
    21901 tagctctgct tgtcgatcca tcacttcatt aaggtccact gtattgactt gagcgatacg
    21961 aacgttggaa ctgcccattg ggaaactttt tgcatccaaa tcaagaagaa ccgcagctga
    22021 tttatcactt agatttgttc ctgctttcaa catttcaagt ccataagtgt ttagatcgac
    22081 ttcagcgatt gctgcaagtt cttttgcagc tgcttcatct tcaggcgtac atgttggtga
    22141 tttgaacaac aatgtatctg aaatgatagc tgagagcatg attcctgcga tttctttagg
    22201 aattgttact tggttttctt tatatagttt taaaataatc gtacttgtac atccaactgg
    22261 ttctgcacga tagtacaatg gattagctgt ttcaaagttt gcaatacgat ggtggtctac
    22321 tacagctaaa atatttactt cgctgatatc tgaaatactt tgttggaatt cattatgatc
    22381 cacaagcatg acatctgcat tttctgcttt agctgactct actactcgtg gtgcagatag
    22441 ctgaaagtgg tctaatgcgt attgtgtttc ttcattaggt gtacctagag caactgcttc
    22501 tgtttcttta cctaattcat tttgcaagtg tgaaaacgca atggctgccc cgattgcatc
    22561 tgtatctgga ttttggtgac caaaaactaa aatttttgac atgtcattcg ctcctttgtg
    22621 atttctccta tttaataata gcaaaaaaac ggaaagaagc aatacttatc ttctttccga
    22681 tgcgacctat cgcgttaaat attttgtata ctcttctgta tgaagcaatc gtttagcatt
    22741 ttcgacacgt tcttttgtag gtggttcgat cccttctaat ggatatttca aaccaagtgt
    22801 ttcccacttg tattttccca tcgtatgata aggaagaatc tctactctgt ctacatttga
    22861 caaggtttga ataaagttgt ctagtcggat caaatattca tcataatcac ttcgctcagg
    22921 aactagtaca tggcgaatcc aaaccggttt gttgattttt gacaaatatt gagccatttc
    22981 taaaatattt tcattgtccc aatgcgttag ttttttatgc tcttcattat cgatgtgttt
    23041 gatgtcaaat aacagtaaat ctgtatactt catcaattct tgaaaacgtg aaaagaaagg
    23101 ttcttcatac gtaaaaggtt ttccgcatgt atccaatgta gtatgcatct tccgctcttt
    23161 tgctttttta aatagttcga tcaaaaaatc gatctgcaat agaggttctc caccactaac
    23221 agtaatacca cctttatctc cccagtaagg tctgaagcgt tctgctttat ctaaaagttc
    23281 atctgctgtg tattctttcc ctccgccaat attccaagta tctggattgt gacaaaattg
    23341 gcaacgcatg cggcatcctt gcataaacac gacaaagcgc agtccaggtc cgtctactga
    23401 accgaaagtt tcgatagaat gaacatagcc gattgttttt tcttccattt aagttcacat
    23461 cttttctttg atttcatttt aagtacaagt tagctgtcga agcgaagctt tctgaacaaa
    23521 atttttcgca attgccgctt cgttatgtgt aaaatttaca gggacggaac atacaagtcc
    23581 tgcccctgta aatcgtttct ttcaaataag catactgatt attcgatttt gatgcgatgg
    23641 ctattcaacg tcctttcgac gctaagtcga aaaacttttt tcacgaactt tttacattct
    23701 gtcgtgtgaa gttctcatca agacatctaa ttgttgttct cttgtcaagt cacggaattt
    23761 aactgcataa ccagataccc ggatcgtcaa gttcggatat ttcattggat tttccattgc
    23821 atcacgcaat aaatcattac tgaatacatt gacattcaaa tgataagctc cttttgagaa
    23881 gtatccatct aagacattac gcaagttttc gattcttgta tcttgttctt ttccaagacc
    23941 tgaaggattg attgtttggg tatttgaaat tccatccaat gcacagccgt aattcatttt
    24001 agctgttgag ttcaggctag ctaataaccc actttgttcc gcattgtaac ttggatttgc
    24061 tcctggcgca agtggttttc cagcttcacg gccatcagga gtatttcctg tatttttacc
    24121 gtaaacaaca tttgaagtaa tcgtcaataa tgaagttgtt ggttttgagt tgcggtaagt
    24181 tttatgacgt ttgatttgtc ccatgaagta ttccaagatc catgaaacta attcatctgc
    24241 acggtcatca tcatttccgt atttagggaa gtcgccatcc acttcaaaat caactgctat
    24301 tcctttttca ttacggattg cttttacttt tgcatatttg atcgctgaca agctgtctgc
    24361 cgcatgagag atcccagcaa tacctgtagc aaatgtgcgt tgcaagtcag aatccatcaa
    24421 agccagctga gctgcttcat aagaatattt atcatgcata taatgaatga tgttcaacgt
    24481 attgacatat agttctgcta accaatccat catattcatg aagttatgtt tcacttcttc
    24541 aaagtctagt atttctgatg tgattggacg atattctggt ccaacttggg cgccagtcac
    24601 ttcatcgact ccgccattga tggcatataa tagtgtttta gccaagttag cacgagcgcc
    24661 aaagaattgc atgtcttttc cgacaactgt tcctgataca cagcaagcaa tcgcacaatc
    24721 atcagaaccc cattgttcac gaagctgatt atcattttcg aattgaatcg aagaactttc
    24781 gatagcgatt ctgctagcat atgttctgaa tccttctgga aggtgttcag aatacaaaac
    24841 agtcaagttt ggttctggtg aaggtcccat atttgttaat gtatgaagca gacggaaatc
    24901 attctttgta actaatgaac gaccatctaa tcccatacct gcaatggata aagtagccca
    24961 gatagggaat cctgagaata attggttata ttcaggtgta cgcgcgaatt tgaccatacg
    25021 taatttcatt actaagtggt cgatcaattc ttgtgcttct ttttctgtga ttactccatt
    25081 atccaaatca cgttggatat agatatcaag gaatgctgag atacgtccga ttgacatcgc
    25141 agcaccattt tgtgatttga ttgctgctaa atatccgaag tatagccatt ggacagcttc
    25201 ttttgcattt gcagctggtt tagaaatatc atagccatat ttcgcagcca tttctttcat
    25261 ttgcgctaat gcacgatatt gttcagtgat ttcttcacgt aaacgaatca cttcttcact
    25321 cattgtatga taaccagtca tatcatgatc ttttttcttt tgttccatca aatagtcgat
    25381 tccatacaat gccacacgac gatagtcacc gatgatacgt ccgcgaccgt aagcgtctgg
    25441 aagccctgta atgattttgt ttttacgagc agcacgcatt tcagcagtat aagcgtcaaa
    25501 tacaccttga ttatgtgttt tgcgccagtc agtgaaaata tgtgtcaatt catcgtttgg
    25561 tgtatagcca ttgcttttta gcgcattgtt agccatattg attccgccga acggcatgaa
    25621 tgcttgtttc aaaggtttgt ctgtctgtag accgacgatt ttttccaaat ctttatccaa
    25681 atagcctgca tcgtgtgaag taatagttga tacgatttct gtgtccatat catagacacc
    25741 gttacgctcg aattggatat catttaactt ttgcaattca tcccataatt tttctgttgc
    25801 ttctgttggg ccttccaaaa agctgtcatc accgcgatat tcagtgtagt tattttgaat
    25861 aaagtctcta gtgtctacgc cttctttcca tttggtacct ttaaatccat tccattgctc
    25921 cattgtatgg acctcctaaa tttctgtttt atgtaacagt ttttacgtga ttagtataac
    25981 acagcaccct atctttgcaa gggttttctt gttttttatt taacaatttt tatgttttat
    26041 ttacatatat ttaaatcaac gttgacatac caacatttaa gcattgtgaa cactttcatc
    26101 tttttttgca aacgtataag gatttattaa aaatgaatga aaaagagacg ccacctgtat
    26161 taatacagat ggcgtctctt cattaaaata ctacaacagt tacgactcga tgaaaatctc
    26221 aggagcatcg ataacttcga tgacttctcc accttttttc tcatccatcg caaatgatcc
    26281 attcgatgtt cgatcaccta tagggaaatc agttgggtca atcgttaact gctgcccttt
    26341 ttgattcatc aaggttattt cttttgatct tccttccgac atatagataa tacggtgcgg
    26401 attttttttc agctcacgca tgaccgtcac tccccgcttc gcacgtgaag tttggctgat
    26461 ttcttgagca agcatgcgtt taacagctcc tcgctgagta acgattacga tcggcgtatc
    26521 accttctgct aacacaagca gcccgttgat aatcctatct ccatctttca aattcataga
    26581 cttcactcca gctgcctttg ctccaacaac gggaacatct gttaaaggat agcgcaaacc
    26641 cattccttga tgcgttacta agaaaacatc taagcctggc tgatcttctt tcagatacac
    26701 agcaatcagt tcatcttctt ccgcctttag cttcatacag ctgagcggac ggctcttata
    26761 ggtgcgccat ggttcgaact ctgtcattcg tgtttgcttg atcaatccat tcttgctgat
    26821 gaatacaaat gttttctctg cttgaatttt ttgatatggg aaaacagcta aaatcgattc
    26881 gtctacagac agattggaga ttgtttgaga aatgtgctca ccagcatctt tccatttcaa
    26941 atcgggtagt tcatggacag ggcggtagat cacatttgct ttatttgtga tcagaagaac
    27001 atggtccaat gtactcaatt cacctgagta tagcaagtag tcgccttctc gcatcccgat
    27061 ttcttctggt ttggaagcac tataagaacg aaggcttgtt cgcttcacat agccttctcg
    27121 cgtcacactg acaattacgt cttcttgtgc cactaagact tgcgtatcaa ttttgatttc
    27181 ttcgatttcg tcttcgattg tcgttagacg agcggaagaa taattttttt tcacttctcg
    27241 caattctttt ttcataacag agaaaagctc tttgtcattt gaaaggattt tattcaactc
    27301 tgtgatttgc gcaatcaagc tttcggattc cttttgaagc tctgtgatat ccgtgttcgt
    27361 caaacggtat aattgcaatg tgacaattgc ttctgcttgt tcttctgtaa attgaaagac
    27421 gtcgactaaa ttctttttgg cgtctttttt gtctttactt tcacgaatcg ttgcgattac
    27481 ttcatccaga atcgataaag ctttcatcaa tccttctacg atatgctgtc ttttttgagc
    27541 tttggctaaa tcgaattgac tacgttttgt tatgacttgc tttcgatgct cgatataact
    27601 tcttaaaatg tctttcagtc caacttgatg cggagtcata tggtcgatag cgaccatatt
    27661 gaaattgtaa ttgatctgta gctcagtgtt tttgaacaaa taattcaaaa ttccttgagc
    27721 attggcgtct tttttcaatt cgacgacaat ttgcagacct gttctatcac tttcatctcg
    27781 tacttcagct attccatcga ttttcttatt caaacggatc tcatccattt ttttcaccaa
    27841 ggtcgcttta tttacttcat atgggatttc tgtgatcacg atttgctgtt tattaccctt
    27901 cattgcctct atctttgttt tcgaacgaag aataactttt cctcgtcctg tttcataagc
    27961 ttttttgatt tcatcttttc cttgcaagat ccctcctgtt ggaaagtcag gtccaggaat
    28021 atattccatc aatttttcaa ggctcgcttg tggatgatcg atcatataga ttgtgccatc
    28081 gatcacttca gctaagttat gagtcgggat ttctgttgca tatccagcag atatacctgt
    28141 cgatccattg acgagaaggt ttgggtatcg agctggaagg actgttggtt ctttttctgt
    28201 atcatcaaaa ttccacacga gatcgactgt ttctttctcg atatctgcta gcatctctcc
    28261 acttaacttg gataatctgg cttcagtata acgcatcgca gcaggtggat caccgtccat
    28321 actcccgtta ttcccgtgca tttcgatcag tacttcccgc aacttccagt cttggctcat
    28381 gcggaccatc gcttcataaa tactgatgtc accatgagga tgataattcc ccatgatatt
    28441 tccgacagat tttgctgatt tacggaatcc tttgtcatac gtattgccat ctttgttcat
    28501 ggcaaataat atccggcgtt gcactggctt caatccatca cggatatcag gcagtgcacg
    28561 ttcttggata atatatttgg aataacgtcc gaagcggtca cccatcactt cttctaaagt
    28621 taattcttgt acttcttgtc gattttccat caggtgacct cctattctac ttcaaataac
    28681 tgattgtttt catttttatc tgctcgttct tcatctaata aatcattaga aactgaagga
    28741 gagatttcag tatcctcttt tttatctaag atactgccat cttcttcgag cgtaaattgc
    28801 acgtgatttt cgatccattt tcttcgaggt tctactttgt cacccatcag tgtggttact
    28861 cgacgttcag cttgcgcagc atcatcgata cggacgcgga tcaacgtacg ggaagtcgga
    28921 tccatcgttg tttcccacaa ttgttccgca ttcatctctc caagtccttt atatcgctgc
    28981 agcatatatc ctttgccgac ttttttaatc attgctgcca attcgtcatc tgtccacgca
    29041 tattcaatga cttgcttctt tccttgtcct ttggatactt tatataaagg cggtaacgcg
    29101 atatatactt tcccagcttc aatcagcggt ttcatataac gataaaagaa agtaagcagt
    29161 aatacttgaa tatgggcgcc gtctgtatcc gcatcggtca tgatgattac tttgtcgtaa
    29221 ttacagtctt cgatagaaaa ctcaggaccg actcctgcac caatcgtata gatcatcgta
    29281 ttgatttctt catttttcaa gatatcttgc attttagctt tttcggtatt gatgactttt
    29341 cctcgtaaag gtagaattgc ttggaacttt ctgtctcttc cttgtttagc agatccgcca
    29401 gcagaatccc cttcgactaa atacaattca tttttcttag ggtttcggga ttgtgcgggt
    29461 gtcaattttc ctgaaagaag agattcgcct tttttacgct tcttcccatt tcggctttct
    29521 tctctggctt tacgtgctgc ttctctcgct tctctggctt tgattgcttt acggaccaac
    29581 atctggctca tttcgctatt ttcctgcaaa taaaagccca tttgttcact gatgacgtta
    29641 tctactacgg tacgagcaac tggcgtacca agtttttctt ttgtttgccc ttcaaattgc
    29701 agaagatttt caggtacacg gatagaaagt acagcagcta acccttctct gaaatcacta
    29761 ccttccagat tcttgtcccg ttctttcagc aaaccaactt ttctcgcata ttcattataa
    29821 gatttcgtca tcgccgcttt cataccagct tcatgggttc ccccatcttt tgtccgaacg
    29881 ttattgacaa aagataaaac attttctgaa taaccgtcat tgtattgata agcgacctct
    29941 acttcgatac cttctttttc tcctgaaaaa taaacaactg gtgtcaatgt atctttttct
    30001 tcattcagat aatcaacaaa ttctttgatt ccttcttcgt aatgaaaaat ttccttgact
    30061 ggttcttctc cacgaagatc agaaagagag attttaacgc cttttagaag aaaggcagat
    30121 tctcttaacc gttcggctaa aatttcatat gaaaatttcg ttgtactgaa tattgtatca
    30181 tctggtaaaa aatggacagt cgtcccgttt gctttttttg tttttcctat ctttttcaaa
    30241 gtaccgtcag gtttaccgcc gtttttaaat ttctgctgat actctacgcc atctctaacg
    30301 atcgttacaa ttagccattt cgataaggca tttaccacac tggctcctac accgtgcagt
    30361 ccaccagaag ttttgtaccc tccttgacca aactttccgc cagcatgcag cacagtaaaa
    30421 atcacttcaa ctgttggtat tccggaagca tgcattccta caggcatccc acgtccgcta
    30481 tctgcaacag ttatactatt gtcttcatgg atggtcacat caatctcact gccataacct
    30541 gataatgctt cgtcaactgc attatcgacg atttcataga ctaaatgatg caagcctcgg
    30601 ctgtcagtgg atccaatgta catccctggt ctttttctaa ctgcctctaa tccttccaat
    30661 acttggatgg acgagtcatt gtattcgttg tttacttttt ttgccaaggc aaacgctcct
    30721 tcaataatac tttgcggcaa aaccgcacca cgattatcat actagaaaag gtcgtgaaaa
    30781 aaaagagggt agacgagaaa tcaattttaa ttatctccta accgcttttt cgacattccc
    30841 ctctatcgat catctcttct ttgtggtcat ttttattttt ggttttcttt caacttacca
    30901 agttcaatct taatacaacg atccattacg atatctgtac gtcctgcttt tcttagcatc
    30961 tcagctgctt ctttgttttc aatccctaat tgtgcccaga acactttagc atcagttttc
    31021 aaaaaatcac ctgctatttc aggtaaaaat tcactccgtc taaatacatc tacgatatcg
    31081 attggatcat cgatttctgt caaattcgca tagactttct cgcctagaat ttcttcacca
    31141 gccaataatg gatttactgg tatgatttta tatccattgt cttgcaatac ctgtgcaacc
    31201 atgtaacttg ttcgttctgg tttattgctc aagcctacga cggcaatatt ttttgcttct
    31261 tccaaatagt tgaaaatcgt ttcttgtgtt ggatttttta cagacatttc tgccacccct
    31321 tgcttttctt tttattccat ctattatact tgtatctttc attttttcaa ttgttcctgt
    31381 tacctattca tgctaaaata aaggatagtt ttcaacttga aagagggtaa aaaatgaaat
    31441 tgatcttatt acttatttta gcatatcttt taggttctat tccttccggt gtctggattg
    31501 gcaaattgtt tttcaaaaaa gatatccgac aacacgggag cggtaatact ggaacgacca
    31561 atacatttcg tgtattaggc aaaacagccg ggattgttgt cctgcttatg gatattttaa
    31621 aaggtacatt ggctacttgt ttgccgatga tcttccacct ttctgggatt aaccctcttt
    31681 ggtttggggt atgcgccatt cttggccata catttccgat ttttgctggg ttcaaaggag
    31741 gaaaagctgt tgcgacaagt gcaggaatgc ttttaggatt taatcctgta ttttttgttt
    31801 attcttgtat catctttatc gttagtttat tttgtacaag tatggtgagt ttgagcagta
    31861 tgatcagtgc agtattgatc actttatcta cgatcgtcct tccttatgta gctcctgtga
    31921 ttttggctaa gcccaattgg ttactgacga tcattgcctt tttagtttct tcctttatct
    31981 tttatcggca taaagacaat atcaagcgta tcagaaacca tactgaaagt cgaatcccgt
    32041 ttggtttgaa tagcagcaag aaaaaatcct gatacttttt cattccaaag agataaaacg
    32101 ggagtgtgac aaatcgatgt cacactccct tattacttac aggatagctt ttttacacac
    32161 cttggtaaac ggtgttccct gctaacttct cggtcacaac ctcttacttc actgtgatgg
    32221 aatattttgt cttgaagatt tcatgggcac ctagttgatt gatccctact ttttccatca
    32281 agtttccact actgttcacc gtgtcagcga ttccgcacca cggttcgata caaacgaaag
    32341 gcgcttcttt tggataagga gaccagatcc ctacataagg catatcttta tagcttaaag
    32401 ttacgctatg ggaagatttt tcacttcgaa tggcgtacgc atttagaccc cttgtttcaa
    32461 aaaccaaggc atcttgctca aaaagttcac gattcaaagc cagattcgta tttgtctgac
    32521 ctaacgtctt ttgatccata tcgatgaatg ggcctttcaa tggtaggcgt aatcttgatt
    32581 tcaatggaga aaatgccaga tagaaatctt caaatgacaa tccttcttca agaggtacat
    32641 tgaaagcagg atgtccaccg atagaaaaga acatttcttt ttcaccggta tttgccactt
    32701 gataatgaac cgtgatgccg tctcctccta attcatagct gatcactaat tcaaaatcaa
    32761 atggatactt ttctaatgtc tccggcgtac tccttaagat gaaactagcc atttcttgtc
    32821 catgttcaat gacttcaaaa tccatatccc gagcaaatcc gtgttgcccc atttgatatg
    32881 ttttcccttg ataaacatac tgatcatctt ttaaacgtcc aacaaaaggg aataaaaccg
    32941 gtgcatgtct gccccaatac gctggatcac cttgccagat atactctaaa ccatgttctt
    33001 ttgatttcag actgatcaat tccgcaccgt gttcagcgat ttcggcgatc agctggtcat
    33061 tttgaataat aacagtcatt gccattcctc caacttcgac ttacttttta aaatgggaag
    33121 gctggaacaa acatttctat ctgccccagt ccccttactt ctaataactg tgttttgcta
    33181 actcgtcttt aaaacgggaa ttcaatactt ttaaataagt tcccttcatg cctaacgaac
    33241 gagattcaat gattccagct gattccaatt ttcgtaaagc attgacgatc acagaacgag
    33301 tgatgccgat actatcggca atattagaag cagtcagacg accttcatct ccatccaatg
    33361 cttcaaagat agcttgtaca gccttcaatt cgctataaga taatgtgttg attgccattt
    33421 ggacagccgt tgcactccgt acatctgctt cgatgcttcg agacttctga taaaggatct
    33481 gcataccgac aaccgtagct ccatattctg ctaagactag atcgtcatct gcaaacgatt
    33541 gttccatacg agataagata atcgtaccca aacgttcgcc agctccaaag atcggaacga
    33601 cagtcgtcag accaaatgga tatttctctc ttgactctac cggaaatgca gtaatatcac
    33661 tagtaattgg gatattagct tcagttttca tcaactgtgc cactgtatca gtataagatt
    33721 caggaaattg tttctcaaca aacattgttt tgattcgttc attgttcaca tcgtgacgtt
    33781 cattataacc tagtaatact ccgtcacttc ctatgatata ggtgttgctg tcaagaatac
    33841 cacctagtgt gacagccatt ttatcgtaag gcaaatctgc ttgcatgtca aaagtattct
    33901 tttgctgcaa aagtgtgttt atcttgcggg ttttcgttag caagtctgtc attgtcgtcc
    33961 tccgattttt tataaaatat agcgacttaa atcttcattt gccgcaattg agccaagttt
    34021 gtcatccaca tatgcttctg tgatcgttat ttctcccatt tgcatatctg gtgcttcaaa
    34081 taatagatct tctagtagtt tttccaaaat ggtatgcagt ctgcgtgcgc cgatattatc
    34141 tgtgtcacgg ttgacatcga aagcgatttg agcgatccgt tcaattgctt cttttgtaaa
    34201 cgtaactgac acattttctg taccgatcaa cgcgacgtat tgttttacta aagcattatt
    34261 tggttcggtc agaatcttca caaaatcttc agctgtcaaa tcatctaatt ctacccgtat
    34321 cgggaagcgt ccttgaagtt ctggaattag atcacttggt ttagacaaat ggaaagctcc
    34381 tgaagcaatg aataggacgt gatctgtttg gatgatcccg tattttgtgt ttacttgtga
    34441 accttcgaca attggcaata tgtctctttg gacgccttct ctggatactt ctccagattg
    34501 ctgtgattta gaagtgatct tatcgatttc atcgataaaa ataattccac tttcttccgc
    34561 taatttgatt gcctcactat ggatatcagc atccttgact aattttgctg attcttcctt
    34621 gatcaataac tctaaagctt ctttgactgt taccgtccgt tccacttttt tcttcggtgt
    34681 caaagcacct agagtttcat ttaggtcgat ccccatttgc tccaatccat tgttcatcat
    34741 tggtgcaggt ttcttttggt cttcaacttc aatggttact tcacgactat ccagtaaacc
    34801 cttttgtagt tgttccaaga ctgtacggcg attgattcga atatcttctg tcacttcttc
    34861 ttgcggttca gcggtatttt gctgactagc attaaaaaga ttcatcattt gttcaaattg
    34921 attagtggat tgtttctgtt cttttttgat accaggtacc aagattttta ccaaacgttt
    34981 ttctgcattt tttttagcac ttgtgtatac acggctgtac tgctcttttt ctacgatttg
    35041 gatcgcattt tctactaaat cccgaaccat tgattctaca tctcttccga cataaccgac
    35101 ttcagtaaat tttgttgctt cgactttgac aaatggtgca ttgacgattt ttgccaaacg
    35161 tcgtgcgatt tctgttttac cgacccctgt aggtccgatc atcagcatat tttttggtgt
    35221 aatatcttgt tgcatttttt catctaactg caaacggcga tagcggttac gcaaagcaac
    35281 ggcaacagat ttttttgctg cgtgctgtcc tacgatatat tcatctaatt cacttacgat
    35341 ttgtcttggt gtttgtttta attcatacat atctactcac ctcttacagt tcttctacga
    35401 taatattatg attagtaaag atacagatat ctgcggcaat atttaacgca ttttctgcaa
    35461 tttctttcgc actcatgtcg ttttgagcat agtttttcat tgctcgggct gcagccaacg
    35521 caaaattccc tcctgatccg atagctaaga taccatcatc tggtgtgata acttctcctg
    35581 tacctgaaac aagaagcatt tctttatcat tcattacgat cagcatcgct tcaagttttt
    35641 gcatagattg ctgtgtccgc cattcttgag ctaattcgac agctgcgcgt tgtagattcc
    35701 cgttatattc gtttagtttg ccttcaaatt tttcttctaa agtaaacgca tctgcaacac
    35761 ttccggcgaa acctacaaca acttctccat tgtagatacg tcgtactttc tttgcagtgc
    35821 ctttcatgac aacagattca cccattgtta cttggccatc tccagccata gcaaatttcc
    35881 cgtctttttc gacggcacag atagtggttg aatgaaatgg tgattcgacc atgtttgttt
    35941 cctcctaaat ttgttctttt ccttattttt acgctcttgg atggaaagaa cgatagtttt
    36001 tttgtaaact ttcttttgtt acatgggcat agatctgggt agtcgataaa ttggcatgtc
    36061 ctagcaattc ttggactgtc cgcaaatctg ccccattatt taataaatga gtagcaaatg
    36121 tatggcgcag catgtgagga tggatatcac tatccaaact gctttttttg atcatttgat
    36181 tcaagacata ttcaattcca gtaggcgtga ttggttttcc atgatgattc acaaaaacga
    36241 tatcatgctc ttcgtgatat tttgtcatca atacctggcg gccttccctg aaatattctt
    36301 gcagagcatc ttgagcaaaa gaacctagag gagcatatct ctctttgttc cctttaccat
    36361 ggatcaacat gacctctgaa tcaaaatcaa tgtctgatac cgacaaactg ctacactcac
    36421 ttaaacggat gcctgacccg taaaggactt ccagtaaagc gcggttcctc aaatccaaag
    36481 gactatctcc tgctacgctg tcaaaaagtg cttgcatttc attttcataa aaaaaacgtg
    36541 gcagtctcaa atttttcttt ttgagatgaa cgtaagaaaa tggattttct tttatcactt
    36601 cttctttgag aagatactga taaaacgaac gtaggctggc tatcttgcga ctgatagaat
    36661 ttctgctata gctaggacga tgatcagcta atgcactcaa atagacacga accgtaaaat
    36721 attccgcctt cagcagatca gcgtctccag attcctccaa aaaatgtttg aaatggagaa
    36781 tgtcttcttc atatgcttct ttcgtttttt ctgaatatcc tctttcgaca atcaaataac
    36841 gcaaaaattt ttgttcccac gtggcttctg tcattttctc accttccttt tgtcccagag
    36901 aataatttag cacagcgttt ataaaaagac aaataaattg tcagaattta accaatattt
    36961 ctaagtttgt tcacaattgg cgcaattcct cacattttgt ttgagaaatt cttaagaaaa
    37021 acgcaagaaa aagagcgaga ctatccgtct caccctcttc atggagtttc cttactcatt
    37081 tgggattgca gttttttcta attctgtttt tattgcctca ttagctttta aagctcgttg
    37141 tgcaattgct tcatatcttg cttttttgtc tcgaatgcgc tctggcagtt caggaaacag
    37201 accatagttg gcattcatcg gttggaaatg ttttccttcc gcatgagtaa tgtagtaagc
    37261 catacttcca atagctgttt cttgtggaaa ctcgactggt tccatttctt ttgctaagcg
    37321 tgcggcgttg attccagcaa gtagtccgct cgctgcactt tcaacatagc cttctacacc
    37381 agtcatctga cctgcaaaga acagatcttc gcgtttcgtt gattggtaag tcggtttcag
    37441 caattctggc gaattcataa agctgtttcg gtgcatcaca ccatagcgaa cgaattcagc
    37501 attttctaac ccaggaatca tccggaaaac acgtttttgc tcgccccatt tcaagtgagt
    37561 ctgaaagccc acgatgttgt ataaagaagc tgcagcattg tcttgacgca gctggataac
    37621 agcataagga cgtttgccag ttttaggatc ttccaatcca actggtttca taggtccgaa
    37681 aagcatcgtt ttgaatcctc gctgtgccat tacttcaatt ggcatacatc cttcaaaata
    37741 tttttctttc tcaaaagatt taagttctgc cacttcggca ttgattagtg cctcatggaa
    37801 agctttgaat tcctcttctg tcatcggaca gttcaagtaa gctgcttctc ctttgtcata
    37861 gcgggacttc aaatatactt tatccatatc gatcgtagct ttgtctacga ttggtgcagc
    37921 tgcatcatag aaataaaaac catctgaacc attgaatgct ttgatgcttt ctgctaatgg
    37981 atgagaagtc aaaggtcctg ttgctactac ggtgattcct tccggtattt cagtaatttc
    38041 ttcgtttatc actgttacta atggatgctc tttgactttt cgggtaattg tttcagaaaa
    38101 agaatcgcgg tcaacagcta aggcaccacc agctggcact gctgtttcat ctgccgaagt
    38161 gattactacc gaattcaatc ggcgcatttc ttctttcaat aaccctactg cattagccaa
    38221 actgtttcct cttaatgaat tggaacatac tagttcagca aaattacttg tgtgatgcgc
    38281 ctcagtcgat ttgactggac gcatttcata taaacggaca gggactcctg cttgtgcgat
    38341 ctgccaagca gcttcacttc cggcaagccc tgctccaaca acatttacca ttttcaatga
    38401 aagaacctct tttctattat ttttggatgt cttcttcgta atctccattt acacagacta
    38461 cttgtttacc gcctttgact ttcttctcga ccagatatcc gtcacatttt ggacaatttc
    38521 tgcccactgg tttatcccaa gaagtgaatt cacattctgg gaaacgggaa caaccataga
    38581 atagtctgtt tttcttagat tttcgttcaa tgacttgtcc ttcatgacat actgggcaag
    38641 tcacaccgat ttcttttacg attggtttcg tattccgaca atcaggaaaa ttactgcaag
    38701 cataaaactt cccatatttc cctaatttta tcaccatcgg atgtccacag acatcacaat
    38761 cgaatcctgc tggttcatct ttgatttgaa ttttttcgat tttttcttcc gcattcgtca
    38821 gttctttttc aaacggttta tagaaacgat caaccacttt gacccacgct tccgtgcctt
    38881 cttcaatttt atccaaatct ccttccattt ctgctgtgaa atggatatcg acgatttgag
    38941 ggaagaattc acagattaat gaattgacaa tttctcctag ttcagtcggc tcaaatcttt
    39001 tttgtgtcaa ttttacatag tagcgtcttt ggatcgtatc aagtgttggt gcataagtgg
    39061 aaggtcgccc taccccgttt tcttctagtg cacgaatcaa cgtagcctca ctaaaacgtg
    39121 ctggtggttg agtaaaatgt tgttttggtt caatatcaac agattggact acatcgcctt
    39181 cttcaagttc tggaagaata ttttctttct cttctttgcc gtcatctcta ccttccacat
    39241 acacttgcat gaatcctttg aatttgattt ttgaaccgtt cgcaatgaag atgacaccat
    39301 tttgttctaa tgtcactttc attgtatcta aaatagctgg tgtcatctga ctagccacaa
    39361 aacgtgacca gatcaatgta taaagtttca attgatcttt atctaaatat tgtttgagtt
    39421 catctggtgt acgaagcaca ctcgttggac gcacagcttc atgggcatct tgtgcgcctt
    39481 gagcattttt gatctttcgg tgatcatgtc tagaaaattc ttttccatac gtatcttcaa
    39541 taaatgctgc ggcctctgct tttgctgtat cggcaatccg ggtagaatca gtacgcatgt
    39601 atgtaatcaa gccgactgtt ccttgtttac ccaaagtgat tccttcatat aattgctgtg
    39661 ctaccatcat ggtttttctt gttcgaaagt ttaacttacg ggcagcttct tgttgcaggc
    39721 tacttgtagt gaaaggattc gcagggtttc ttttgcgctc tttcttttct acttttttaa
    39781 cttcgaagtc tgttccatca attctagcag tgacttcttt tacactttgt gcatctggta
    39841 atttcttttt cttaccgtcg attccccaga aatttgcttt gaatttcttg cgagcttttt
    39901 taaaattacc gtcgatgctc caatattctt ctggaacaaa ttctcggatc tctttttcac
    39961 ggtcaataat aatttttaaa gcgacagatt ggacacgtcc tgcacttaac cctttcttga
    40021 ccttacgcca gagaatagga ctgatcgaat aaccaacgat tctgtctaag gtacgacgag
    40081 cttgctgtgc atctactaaa tctacatcga tcgttcttgg ttccttaaaa gctgctttga
    40141 ctgcgtcttt tgtgatttca ttgaagacca cccgattttt ttctttcaga tccaacccaa
    40201 gtaggtacgc taaatgccaa gcaatcgctt ctccttctct atccgggtca cttgccaagt
    40261 aaactttttg agcttttttt gctgcggctt tcaggctttt gatgacatcg ccttttccgc
    40321 gtatagaaat ataatggggt tcataattgt tttcaaaatc gatacccatt ttacttttcg
    40381 gtaaatctct tatatggcca acactggcca ttactttata gtttcgtcca agatatttct
    40441 caattgtctt ggcttttgca ggtgattcta caatcactaa atatttatac gccattactg
    40501 aatggactcc tttccattgt cgtgttccta ctattaaaag aggattcata caaatcgtag
    40561 ctaatcatat ccattcagaa aacgtttgtc aagaaaagca aatgaaaagt atggtgttat
    40621 tctttaaatt ctgccaagat atcttcaccc gaaaccgtgc aaactgcacc gtcttggatc
    40681 aaatgatgac atccggtaga atgttctgtt aaaatatttc ctggaacaga aaaaactgtt
    40741 cttccaagat ccaatgcttg ctgtgctgtt atcagagagc cgcttttttt cttggcttca
    40801 aagacacatg ttcctttaga caaagctgcg ataatccgat ttctcattgg aaaacaatgc
    40861 tttttcgggc cggattgagg aggatattca ctcaaaagaa gatgatcatt tgcaatcacc
    40921 tcttgcaagt cacgattttc agcaggatag acttgctcta tccctgttcc aatgactgcg
    40981 attgtccgtc caccatgaga gattgctgct tggtgaccgt atgtatctat ccctttcgcg
    41041 cagccactga cgatcacata tccagcttct atcactggtt ttagtaaata ataaactgtt
    41101 tttttggcta aagcagaagt gtgtcttcca ccaacaacgc ttaacatgtt acaagaaagt
    41161 aaagatagat caccattata aaacagtaca attggcggat aggtcaagtg aagcagctga
    41221 gggggatatt tgggatcaaa gcaagtgata aacgactgtt tcgattggat tttgccccag
    41281 ttagcgtgaa tttcctgcca acttttgcga aacagctccc ggtgtttggt tattttacct
    41341 atcttaatga tgtcttcaaa tccgatttct ttataaggat actgcatcat ccagttagca
    41401 agtgcccatt ttccttgact actgattcct tttgtataag caagtttcaa taaattttct
    41461 tctatttgat acatcataaa accgcctttc gtctctcttt ctaataagat acgtaaaagc
    41521 ggctgttttt tattgtaaat atttttttac aggtgcaaaa cttttacgat ggattggcgt
    41581 aattccttgt cgttcaagtc cttctaagtg ttcctttgtt ccataacctg cattatgcga
    41641 gaaaccatat ccaggataaa gcacgtcata ttctttcatc aattggtctc gatatacttt
    41701 agcaatgata cttgcagcag caatcgacac agatcgtgca tcccctttga ttattttttc
    41761 ctgctgaata ggcaaatcca aggtcatcgc atcgatcaac aaatagcttg gtacaatgga
    41821 caatttttct actgcttgga tcatcgctaa tttacttgct tcataaatat tcacatcatc
    41881 gattgtctgc tgatcaacga tcccgatccc gattgccagg gctttttctt gaacttgttg
    41941 ataaagaagt tctcgctttt tctctgataa ttgtttggag tcattcagcc ctaatatgat
    42001 ctcatttttc ggcaagataa ctgcagctgc cacgacaggt ccagctaatg gtcctcttcc
    42061 tacttcatca atgccggcaa tccattggtg accttgtgcg tacccttgct tttcaaagca
    42121 aaacatttct tcatattttt ccttaattaa tgcttccttt ttcaatcgtc gttcaaaaga
    42181 agccagtgct tgctgcacgc ctttgcgtgg atcatttctt aatgattcta gtgtaggatc
    42241 atctattctc tgtatgcttt tcaatcgttc tttgatactt tggatggatt cagtcatcac
    42301 ttttttgctc catttcttcg caacgatcca gtgtgtatcg tccaagtttt ccttgacgaa
    42361 tatcatgcac gactaattcg ctgccacgat catagtcttc tttataccca cgttttgctg
    42421 taatatccat cagtaattcc ggtgccaatt tttcttcttg ctcgattgat aaaccataac
    42481 gatcgctgat tcttcctgaa taatatcggg caaaaaagtc taatccgtaa atcgctaaat
    42541 catctaaatg gacaagctgg tctttgatcg cgcctgtcag cgctagtttt ttgccgattt
    42601 cttcatcttc aaatttgggc cataaaatcc ctggtgtgtc taacaattcc aactcattac
    42661 ctaaacgaag ccactgctga ccttttgtta cgccaggctt atttcctgtc tgtgcaattt
    42721 tcttgcctac tagtcgattc atcaacgtag atttcccaac atttggaata cctaagacca
    42781 ttgcccgaat agcgcgaggc ttgatccccc gagcagcatc tcttgcccgt ttttctttca
    42841 atgcttcttt agccgcaggt actagacctt taattccttt actttcttgt gccgtgatag
    42901 ctaatgcaag atggccttgt ttacgaaaat attcttgcca cagctttgtc tgttgtggat
    42961 ctgccagatc cgctttattc aataaaacaa ggcgaggttt ttgctgaatg atctgatcta
    43021 gcattggatt acgagaagat aaaggtaatc gtgcgtctac caattcaaat acgatgtcca
    43081 catatttcat tttttcagaa acttctcgtc gggcttttgc catatgtccc ggaaaccatt
    43141 ggatggtcat tgttctctcc attcctttca taccggaata ttccccggct ttacttgttt
    43201 ttttcttttt caatataaat tcactttttt attataaaga aattccatct attagttaag
    43261 tgaaagtgaa ccagtcctca ttctatcaca tcttcacttt tttgatgatg caaattttta
    43321 ttttggtttc tatatatgaa tctaaatttt tgacagcgtt tcctaaataa gagatgatag
    43381 acgaacaaaa cagaaaggag acgaaacaaa tgaatagtat ggtttttgtt aattttcctg
    43441 ttgctgatgt caatcgttcc gttgcttttt atgaaaagct gggttttaaa aaaaatgaag
    43501 aattttctac tgaagaagct ggttcaatgg tatgggatga cactttttgg ataatgcttc
    43561 tctctcatga tttttataaa aaatttataa aagataaaga aatagctgat aataaaaaaa
    43621 caagtggtgc tttgatttcg tttagtgttg aaaatgcgga tgaagtaaaa aaatttgctc
    43681 aaattgcaaa agaaaatggt ggtactttcc acgaagtaga tatgggaatg cctgaagaag
    43741 aaatgttttc attagaagtc caagatctag acggaaatac attagaaccc gtatggatga
    43801 agatgtaagc agataactaa tctgacgttt tttataacaa aaaaacaaag gcggatgata
    43861 cgataagtat tctccgcctc tatttttttt attcgtacaa tctctttttt acagtttata
    43921 aaagcagtct tctcaccctt tttttaattg ttagatgcca gtgtatgtaa atcaccaatg
    43981 tgtcatgaca cattggtgat tttatggtat ccttttatta ttaactgaca aaggagagaa
    44041 tattttgaac actttgattg caaaagacgt ttttagattg cccgaaatga atgcaagatt
    44101 gttgtcgacc tctgctcttt tattagccct tgatgtcatc ttatacaaga tagcgattgg
    44161 tcctgcttat tttcaaatca cattcggatt tttaagtatg gcgcttatgg gttatctata
    44221 tggaccgatc tgggctggtt tgcttgaaat gttgtcgaac actttaaatt tcacgatatt
    44281 tggttcagga acgtttcaag ctgcttttct tatcacggct ttccttggtg gatttattaa
    44341 tggcttattt ttatatcata aaaagataaa cctgccttcc gtcatcgctg cccaattatt
    44401 gattatgatc ccagtcagcc tttttatcaa ttcttggctg atgtctgttt tgtttggtgc
    44461 agatttcaaa gtaatttttc taggtagatt catcaggaat atgatccaaa tccctattca
    44521 aataatggtt gtttattcat ttatcaaaac tttagaggct agaaaagtgt ttaaacgagc
    44581 tgtttgattt tacgtactaa ggtagtaaac tgattttgga ttaaacaata aagcgtgcag
    44641 tctcaacaga ctgcacgctt tattgtttct cactggatcc attttatttc atcaagtgga
    44701 taatagacaa atcttgcttt acctagaata tcagttttat tgatcgtgcc aaacgaacga
    44761 ctatcttttg aaactcttcg attatctcca agtacaaaat aattatcttc cggaagcttg
    44821 gattgtcccg ttagctcttg tagtgtaaag tccgtcgtat aggatgcatt tgcatgatct
    44881 gatttcaggt ttttagttag ataaggttcc ttaactactt tcccattgat cttcaattga
    44941 tcattttggt agctgacatt ttctcctgga agaccgatta ctcgtttgat ataaatcgtg
    45001 ccatctgcta attgaaaaac aacaatatca aatcgtctga tctccgaaaa tttttccatc
    45061 acaaccatat caccttgctt caacacgttt tccattgaat tgccttccac tggtaccggg
    45121 atgaataaaa atccccgcaa catgaacgca aggaatacag aaacaagtaa gtatttgaat
    45181 acaagccaga agcgattaat atagcgttgt ttttttgtca atctctatca atccatccct
    45241 ctttttttca ttctttcttt attataagaa aaatatcaaa aaaatgcgac tagatagctg
    45301 aaataaaaat aattctttca attaggctat tggacaaaaa acttttttga tcataaagta
    45361 caaaactatc tcttatatgt catgattatt ttctaaatat ttttcccatg cttcgtcaaa
    45421 gatatccatg gacggcaaat agtcgacatt cagttccaga taattagaaa tttcttcgta
    45481 gctagagctg tgcttaggaa attggatgtc tttaccggct tctgtagcga aaacagataa
    45541 agcatcatgt gtgccaccgc ctcgcagtgt cattaaataa tgaaaaaagc ttcgattcac
    45601 ccttcgttcc tccaattttc ttcgataaag gcagcccggc gtgcatggtt tcttttaaaa
    45661 ttttcaggat gctttttata atactcttga tggtcttctt ctgctagata aaatggttgg
    45721 gctggttcga tttttgtaac gattggctgt tcgaaacgtc cactttcagc taactgtttc
    45781 ttactttttt cagcaatgat ccgctgttct tctgtcaaat aaaagatcac cgggcgataa
    45841 ttgtcaccgc gatcctcgaa ttgtccgaaa gcgtcagttg gatcagtttg ctgccagtaa
    45901 agttctacta attgttcata tgaaatcaat gttggatcat agataatctc cactgcttct
    45961 gtatgtccag tcgtttggct ttttacttgt tcataagtcg gatctggtac atgccctcca
    46021 gtatatcctg acatcactgt atggatacca ggaagcgtgt cgaaaggctg gatcatacac
    46081 cagaagcagc ctcctgcaaa tattgctcgt tccattatga ttccccctct aatactactc
    46141 atccgatgat tttttatttt cttttgtctt gctttcagta ctgctggtgc tagttttgtt
    46201 ggtatttgag ttgtttgaag catcagaggt atcttttggc aaatatagac taaaccgaat
    46261 gtcatcattt actaagtcga ttttatctgc tttaatatac atcccgtttt gcattcggaa
    46321 ttgatccaac ctaagcatga tagtcagatc atctgtattg acttcgatcc aatctggtaa
    46381 tttatagttt ctcttgatca tattcatcac atctttgatt ggcaaactca atgttccgat
    46441 agaaagactt ctagctttta gttggacatt tccgttttcc atcacatacg gatcaaaata
    46501 taaatagaaa tcgataggaa aacctagtac ttcgaaagta ccattgagta gtgcttcatt
    46561 ttccaaataa aatttatatt caatatcact atctttttga tactcactaa ggaaaaagct
    46621 gatgatttga ttgacttttt ctttatttga attgatcgtt aagacaggat ctccttgtct
    46681 ttccgtaaga gctggtattt ctgggagttc tggttcccga ttagcaaaaa tacggctgcc
    46741 taaaaatacg gtcgaaccaa gtactacccc aagaaggata aggaaggcga atttccagta
    46801 atttgttttc tttgttttgt ttgaagatac gtcagatctt gttttcatgt ttattgatca
    46861 tcctccttct ctaaccattg gttttgtgtc tcgatcagct gatctctcac tgcatttgcc
    46921 atcaattgat aacctaaatt atttggatgg aaatgatctt cttcatagag cacattattt
    46981 ttgatgccat ttagattttc tgtcgtgcta gttgtttgat ctgttccttc ttctgtgaca
    47041 cctacttcct gattcaatcc ttgataaagc aaatcattga ttgggataaa gtagcagttc
    47101 ggcatttttt ttgtcaccgt ttctgttcct ttgttccaat tgttaacaat tgtctgcatg
    47161 tcagtgatct caggaaagtt caaataaaat ggattgtaga tgcctaaaat gtaaataggt
    47221 gcggttttat tgagggatcg aaattcttcc aatagttctg tgacattttc ctgatagttt
    47281 ttcagtggct ttttaaagga attgaccgtt aacccgaata aattattttg gataactttc
    47341 atcaaatcat taccgccaac agtcagtgtg ataaaatcag ctgttttgat attgtttcga
    47401 atttcttctt ttgtttttac tcgctttaag atctggtcac ttcgttcacc atttacacca
    47461 tagttttctg tttcaaccgc ggtcaaatta taacggtctt ttagatcatt tgcgacaagg
    47521 ggaacgaatc ctccttgctt ggtttgatct cctactcctt cagtcagcga atctccgata
    47581 gctatgtagt gaagtttttc ttttttattt ggatcttctc tcacagtagt tactggtttc
    47641 attagtgggc cagctttcgg tatagaagca gctaaacctg catagatgat gatcgcaatt
    47701 aataatggga acaaaacgag gatcaaatgt cgtttcgtta atttcatttt ttcacctctt
    47761 gtattcgatt ggttctaatg ttatcaaaaa ttttatcaaa aaggtagtct ttatgaaaaa
    47821 agaaaacaat tattttccta attttataaa gaaaaagaga ataagctgca agcctttcag
    47881 gctgagggct tatcctcttt tcgactaatc tacataatac atgattgcaa acgcgtcttt
    47941 accagtatgc gtagcgataa ccggattcgt atgcagtaca gggatatgca tgtccgggaa
    48001 taattcttgc aaaccctctt tgaattcttg cgccaattct tttgcatccg catgtgaaat
    48061 accgatttgt cgcacatttg gcaattcttt cagttcattt ttcaaattat taaaccattt
    48121 atcgaacgtt ttctttcctc gccccttagt tacagggatt aattcagaat gggcgaaatt
    48181 catcaccacg cgtatgttca aaacactaga cagcaaacca gttgttcggc tgattcttcc
    48241 accttttacc aagttatcta gtgtggatat tccgatgtat aattttgtat tgtcttgtac
    48301 ttttttgact tcttcaagga ttttctctac tgttttacct tcttttgcta attcagctgc
    48361 tcgaattacc tgaaaagata atccttggtc agtggtatca ctgtcgataa ccgttacttt
    48421 cgtttttgtt agattactgg cttgtcttgc tgcttctact gttccactca agccttttgt
    48481 catatggatc gaaatcactt cactgccgtc ttctcctagc tcatcataca tcgagatgaa
    48541 ttctccaatt ggtggttggc ttgttttggg cagatttttt gctttcgcca tcatttccat
    48601 aaatctttcg ccgtctagtt ggtcatcatc gggatatact gttccgtcga tcatgatgga
    48661 tagcggcatg acacgaatgc ctaaagcatc gcgtacattt ttttccatcg tacatgaaga
    48721 atctgttacg atttttacat ttgtcattat tttctctctc tctttcttta acaagatgcc
    48781 ttattcatgt taaactacat gtaagacaat ttctattttt agtataacag acaaataatg
    48841 atttttcatt aattacacct agtaaaggag ccgttttcat ggaaaaaacg aaatttaatc
    48901 gaacgtatga aatattaaat gaagtgttta atgcagtcac ccatggaatt gggactggat
    48961 tgagtatcgc gggactagtg gttcttttag taaaaggtgc ccacttgcat tcctccctcc
    49021 atattgtctc ttatgctatc tatggatcga tgatgattct tttattttta acgtctactt
    49081 tgtttcattc attgattttt acaaaagcaa aaaaggtgtt ccaagtattc gatcatagtt
    49141 cgatttttct tttgattgca ggaagttata ctcctttttg tttgctgagt atccgaggat
    49201 ggctaggttg gactttattt gtattaatct ggttgtttgc gattcttggc attatatata
    49261 aagcgttgac gttgcataaa cgggagagag tatcaaaagt atctacgatc atctatattt
    49321 ttatggggtg gctctgtgtc gtggctgctg ttcctcttta tcactctctt ggaatgactg
    49381 gtgttatttt gatggtagca ggcgggcttt cttacacgat aggcgcattc ttttatagct
    49441 tgaattctat ccgctatatg catgttgttt ggcatttgtt tgttatgtta ggtgcaggtt
    49501 ttatgtactt ctctgtttta ttctttactt aataaaaaaa ataaagctat ggatgggaca
    49561 gctctaaata acagctagtc tatccatagc ttttttatat cgtcaattcg cctgtcccgc
    49621 tttttacagg attctcatta cgctgatacg tttcaaattg atgcggataa cgattttttt
    49681 catccatttc tccttgacta gtattgatca gtgaccaatc agaccagtca acaggtggaa
    49741 aataagcatc tccatcgaat acatgatgga tgactgtacg gtataatatc gtacaaaatg
    49801 gcagaaattc ttgataaaca gctgaccctc cagcgatgaa aaagataccg tctgtttctt
    49861 tttccagagc aagcacgtct tccacactat gcatgacatg cacgccttct gctttatagg
    49921 aagaatctct cgtcaaaaca atcgtctgac gattcggcaa cggacggcta cccatccctt
    49981 cgaatgtttt tcgtcccatc actagtgtat ttgcttctgt catttgtttg aagaatttca
    50041 gatcatttgg caaacgccaa ggaagacggt cttcttttcc aatcaatccg ttttcatcct
    50101 gtgcccaaat tgcagctaac attttctcat cctttctgat tctaaacagc gataggagct
    50161 ttgatcgccg gatgtgggtc atatccttct aattggatat cttccatctc aaaatcaaat
    50221 actgactgct tttcctgatt caaaactaaa gtcggaaatg cgcgtatttc tcggctcaat
    50281 tgttctttca tttgttcgat atgattgata taaagatgag catctcctaa tgtgtgaaca
    50341 aattcgccaa ccgctaaacc tgtttcatgt gcaattaggt gagtcagcaa cgcataactt
    50401 gcaatgttga acggaacgcc tagaaacaca tccccactgc gttgatacaa ttggcagctt
    50461 agccgtccct cttgaacata aaattgaaac atcgtatgac aaggaggcaa agccatgcta
    50521 ggaacatctt ccggattcca tgcagaaacg atcaatcttc tagaatcagg agtctttttg
    50581 attgcttcga tcacttgttt gatttgatcg ataaagccac cgtcttttgt ttcccagtga
    50641 cgccattgat agccgtagat gtgtccaaga tcgccgtatg tttcagcaaa ggaatcatcg
    50701 gttaggatct tctcgcaaaa aagcttgctc tgctttttat attcttcatt gaattcagga
    50761 tctgtcaaac atctatggcc aaaatcagtc atatctggtc cattatagtc tggacttttg
    50821 atgtagcgtt caaatgccca ttcatcccaa atatggtttt tattttgtaa tagatagcgg
    50881 atattcgtat ctccttttaa aaaccaaagc aattcgcttt tgatcaatcc aaaaggtact
    50941 cgtttcgttg ttaacaaagg aaaccctttt tgcagatcaa accgcatttg gtatccgaat
    51001 aagcttaatg tgcctgtgcc tgttctgtct tctttacgat gcccttcttc caacaatttt
    51061 cttcctaagt caagatacgc ttgttccatc ctttttcctc ctaatcttct acccattcac
    51121 tcaagtattc ccaccgtgcc attttttctt caagttgtgc ttctgtttca gatacgtcgt
    51181 tttgcaattc ttgtaatcga gtgaaattat ctccttgatg attcatctct tcttggagca
    51241 atgagatttt ttcttcaagt tctgcaatct catcttcaat cgtttcccat tctttttgtt
    51301 ccatgtaaga taatttcttc ttttcagcag gttccttgct ctgtattttt ggtttcgctt
    51361 tttcagcagg ctgctgggtc tctttttgct tcagtaaata actggacatg tttccgtaat
    51421 aaagttcttg ttttccgttt ccttggaata ctaataactt atctgctacc ttgtccaaga
    51481 aataacggtc atgggaaaca gtgatcacag cacctggaaa acttctaaaa taatcttcca
    51541 aaatcgtcaa tgttgctatg tccaaatcat tggtcggttc atccaataat aaaacattag
    51601 gttgctgaat cagcagtttc aacaaaaata accggcgttt ttctccgcct gaaagtttgc
    51661 ctatcagtgt cccatgcata aatcttggaa ataaaaatcg ttcaagtaat tctgctactc
    51721 cgatttgact gccatctgct gtttttgctt cttcagcagc ttcttgtaag taagcaatca
    51781 tccgtttatc tggtgccatt tcttcatttt cttgtgtata atacgcaaga tgaaccgttt
    51841 caccgataga cattgtaccg ctatccaggg gaattctgcc agctagaata ttaagcaatg
    51901 tcgattttcc cgcaccattt ttaccagtaa tacctagtct ttcccgagat tgaatcaata
    51961 aatctaaatg ttctagcagt gtttggtcat ttatacggta actgccatct ttgatttcca
    52021 gtactttttt ccctaaacgt tgtgtcgcta ggttcagctc taaatcatct tcttggttta
    52081 cttggtacaa gttctccttt agatcttcga aacggttgat ccgtgcttgc tgcttggtgc
    52141 cacgtgcctg tacgccagcc ctcatccaag ctaattcctg tttataaagc cgcttgcgct
    52201 tttcttcttg ttctacttct acacgatcac gttctgcttt ttctaagacg taagcttcat
    52261 aatttccttt atactcgtat aattttccaa atgaaagttc gaagattcgg tttgctactc
    52321 tatctagaaa ataacgatcg tgggtaacca tcaatagtgc gccacggtat tgtttcaaat
    52381 agttttccaa ccactcgatc gcttcataat ctaaatggtt tgttggttcg tctagtaata
    52441 gtaaatcggg ttcatcaatc aacacttgcg ccagactaac tcgttttttc tgaccacctg
    52501 ataaagtact gattttttta tctaattcac tgattcctaa tttttgcaaa ataatctttg
    52561 cgttggtatc tgtagtccaa gcatcttctt tattcatttg ttcttctgct ttagcataac
    52621 gtttttgcac atcagggtct tctccgtttt cccctagttc gatcaatgct tcctcatatg
    52681 ctcgaacagt ttggatcaag gggctgtttc cttgaaagac tgcttggaga acagttaatt
    52741 catcggaaaa ttcagctgct tgagaaagat aaccaatacg gtaatcagtt ggataaaaca
    52801 ctgtttgccg atcaccgtcg ctgctatcga taccggcaat aatatttaag agacttgttt
    52861 tacccgtccc gttcgtccca atcaacccga tacggtcttt ttcgtggacc aaaaaagaaa
    52921 tctggtcaaa caaatctttt tcaccataag tttttttgag gtcagttact tttaattctt
    52981 tcaacatgtt ccctccttat cttggatatt gtatcataaa aatagtgaac ttcaggaaaa
    53041 tttttttggt atttgttctt tttgtgttct caaaaaaaga acaggaccga ttcgatcctg
    53101 ttctgatatg ttttgttttt tataaaactt gaaaattttt acctttttat tttattggac
    53161 tcgttttttt tgactttgct cgaaacgcaa atcggaaaga aaagttatca gatggtttgt
    53221 tcatcgtata agattctaac aaattgatat tcctgcgata gctgttcgca gaaaacgtcg
    53281 tgattttttc agcaacttcg tattcatcaa tgatttttct ttcaagataa taacctctta
    53341 gcattttttt cgtaaacaga ctatcttgct ggatcatcca cgaagaaatc aaattccccg
    53401 agttcatttt tattccttca agtttgcgtt gctggataaa aaattgaacc atcgtttcat
    53461 cataaatatc agtcaagtta tctaaacttt caataatcaa ctgagtattt ttttgatatg
    53521 tctctctgat ttcttggata tcttcatcac taataaatgt ctgacgatca gcatatcttc
    53581 tttgccaaaa cattttcgga tggagaacga ttcgcattaa cctacggaca acaatgaacc
    53641 aaaagccgat aaaagacaaa atctggcttg tgatagaatg ttcgaatcga gaaagaaaac
    53701 gggagtaaaa acgataacca ttagaagaaa gcttgcccga acgataactt tcatccaatc
    53761 catcccgttc gatcgacaaa atcagtgctt gtatttcttg tacttcttgc ttttcatcat
    53821 cggttaaatc ttccaagtac agatcacgta tccgttcttg ataattttct atcactgctt
    53881 cagtggctaa aaattctttt gtattacgat caatttcctt tatttctttt cgcaaaactt
    53941 caacgacctc ttctaaaatc atcaaagcat tcaaatccgt gcttttcgct tcctctgttt
    54001 ccgtcagcat aggcaagaca atgatcccta tgaccaaagt cactaagatg acacccgccg
    54061 tcagaaacaa aagcaaagac cgctggtaaa acatcatatt attgattgaa aatggcaaaa
    54121 taaaaatcgt agccaaactg actgttccct ttacaccgcc aaatgttaag atcaagatct
    54181 cgttcaactg ttttttgaac tttttactgc catctttgaa tacataaaaa agactgatag
    54241 aaatgaatct gatcacaaaa agcatgacag agattaatac gataatcgct aataaaagac
    54301 cgtttggata taaaccgtct ccccatactg gcgagaacac ttgcgtcaat tcgattccta
    54361 aaaatataaa tactaatgca ttcagtgtaa aagcaatagt tgtccatgta ctgtgggaca
    54421 agctcgacaa ttctgcatca aacacagaga ttttcctaaa accagaagct tggagaatac
    54481 ctgctgccac tgcagcaatg attccagata catcaaatgc ctctgccaat acataggcta
    54541 gaaaaggaag caaaagttcg atcagcagat accctgtgac atcttgtgct gatgcttttt
    54601 cgatcaaatg aattattttt tgtttgaacc agacaagcaa aaagccgatc aaagctcccc
    54661 caatactaga aactactaga ctcatccccg cgtttactgc tgaaaaagat ccggtgatca
    54721 gcgcaccaag agcaaactgg aaggcagtaa caccagatgc atcattcaaa agaccttctc
    54781 cttccaaaat atgcatcgcc tttttaggga tattcactcg tccagaaaga gaactgactg
    54841 ctaccgcatc tgtcggacct aacgctgctc caaaagcaaa gcaggcagca agtgggacac
    54901 ttggcaacag gaatgataac gtcgcaccta ccccgacaag ggtcaaaatg actcctccaa
    54961 atgccaagaa caaaattgtc ccgaaatttt tgagaatcga agaaatatct gctttttctc
    55021 cttctctaaa taacaatggt gcaataatca taactaagaa caattcaggt tcaaaatcga
    55081 ttgattgtcc ccactctgtc agacctaata ccactccaag gaaaatttgg atcagtggag
    55141 ctggaatcat tggcaaaatg cggctagcta tgttcgataa ggtgatcgtt actgcaaata
    55201 caataactaa ctcagcaaat tccatcgtcc atccccttta ttttttattt tgtgtaagca
    55261 tttttaaaaa ttgttctttt ttctttaaaa ttttggtttc gactacttca acctgtgttt
    55321 ggttcccctc taattcacac tgtacacgtt ttgactccaa acgaagcaga tcatgtttca
    55381 gacgttccaa ctgacgatac atcaatatat ttcttttacg caacttacaa agtatttgac
    55441 ggcggtcatc catcgaaagg gcgccatagc gatcatgcaa cagttttttt tgttgttcaa
    55501 aactctctct cattttccag atctcctata cacaaacttc catcattata gcaaagttct
    55561 ttttggatga aaaatatttc attgtttcat cgtttattgc tagttagaaa tttccataaa
    55621 tttttcaacg cctcattcta ttccaaatag tatcagatat caagtaaaac ttaagaaaga
    55681 taaaagtttc tgacaaaagg atgtttactt tcccattcct gcctttcttg ttcttttatc
    55741 acaacaaaaa aatggggctg cgacaaaaat cttgtcacag tcccttattc tgaataaacg
    55801 gtgtggaaga gacagcctat cagtagtaat aagacacatc tctttcacag cctcattttt
    55861 gcgtaaaaat cgatttattc gaaaaattcc ccgtatacat cttctacacg ttgatctgga
    55921 ttgatcacga tatacaagtt acctgttttt tcgctgattt ttgttgattg atattgattg
    55981 tctaatcctt ctgttccttt atctagatat gggaagtaca caagatcagg tgtgccattt
    56041 cggtaaagga tttccattcg ctcgatatct tcgtatttca atgcgcgatt aaacatgccc
    56101 atttccagtc cgcctaggtt gatatcatgt gtcgcaactg cgtccccttc atggaagatt
    56161 tcgattttaa atccagcaca cggatggatt tcaacaaatt cactgccatt gattcgtcca
    56221 aaacttgttg tgatttgttt gatccaaaga tcgccgatat gacggcggtt gatcgtccag
    56281 gtttctccat ttctaaaata aaattttagc gctgtcattt ctttactcat taaaaagact
    56341 cctttatgat gtaatttatt cttgtactac ctcagtttag cacaaattta tgtaagtggt
    56401 cacatcttat gttttttctt ctaatgacgt atctgcccac tttaagtagc caacaaagct
    56461 gagcagatac agtctagtgt tttttaaatc aatttttctt tcgataaaaa atccaaaacc
    56521 tgttttttat cattttttat tctttgttct acgacggctt tttcagctaa ttgcaaagct
    56581 tcactaaccc acttaccagg tttttttcca gtttcttcta gcaagatttt accatctact
    56641 gctaattcgc ttaatgactt gataggtaaa cgatcatagg attctcctac ggcatccatc
    56701 tgaggtgttt cgtcaaaata gatcatcaat tcttctactt gcagtgcatg ttttttgcct
    56761 aagaagtaca attcgaatgg ttccaaagct ccagtttctc gttgtcgtag tccttggaca
    56821 agtgcttgaa cgtctttaat catttggtta gaacacttcc acgatttcaa aaaggaacga
    56881 atctcatttt cttttagatc cagttggctg atcaataatg cccatgcttg gctctcttct
    56941 ttcaatggga cggatggtaa ttcagcaaaa cgtaaaagtg cttcgccttc ttctttcaaa
    57001 ccgggacagc aaatgtagca ctctgtttca ataaatgcat ggattcccgc ggcccgttca
    57061 tttcccaaga gcatcttgat gaattccaca tttatacgtt ctacagatat tttacctaat
    57121 agctcgtggt tttctttgat ggcgtagaag gtttcttcct ctaatttgaa ccctagttga
    57181 ctgacaaaac gcaacccgcg catcattcgt aaggcatctt catggaaccg ctcatgagga
    57241 ttgcctacag ctcgcaagat ctgttcttct agatcttgca gaccatcgaa caaatcgatg
    57301 atttctccat cttctttcaa agcaaacgca ttgatcgtaa aatctcttct tttcaaatct
    57361 tctgccaacg aacgaacgaa ttcgacatga tctggtctac ggtagtcctg ataagttgat
    57421 tccgtccgaa atgtggtgat ctcatattgt tcatcatttt ctaataccaa cacagttcca
    57481 tgttcgatcc caatatctat cgtttttgga aaaagctgtt tgatttcttc tggaaatgcg
    57541 cttgttgcga tatccacatc atggattggc tggttcagca agatatcacg aacacttcca
    57601 cctacaaaat aagcttcata tcctgcattc tctaattttt tcagaactgg aattgccttt
    57661 tggtattcca gaggtaattg ttttaatttc atttttccaa cctttcctgc gtgtttcttg
    57721 tcctgagata aagactcagt tgcatggtga ctgtttgaca agtccgtttc agagtcttca
    57781 aggccaggac cacttcatct tgtttactcg tccttacgct caaagcgatt atggtctctt
    57841 tgattgaatt tttccatgtt tttttcaaaa acttcagtta agtcgatatt caatgaatta
    57901 gccatgatca tcgtaacgaa taaaacatca ccaagttctt ctgctaccga gttaactggt
    57961 tcagaagcct ttttgctttt ttctccataa gtgtggttga cttctcttgc caattcacct
    58021 acttcttcag ttaatcgagc catctgagct aatggagaga aataccccac cttgaattgc
    58081 tggatatatt catccacttc ctgctgcata ctgtataaag aacgcttttt atccatcttt
    58141 ttccctccaa aactatctta ccaaaacttt aacgcaaatc ctagataaga gcaattgaga
    58201 aaatcgaatt tccatgctac atttaggaag gaatttctaa atggaaattc tgtgaaaata
    58261 cataattgaa agctggaatt catatggtca aacgaaaaac agtaaaagaa gttcttttta
    58321 tcattatagg aacaagtatt tatgcatttg gtcttgttta cttgaacatc gctaatcatt
    58381 tagcagaagg gggagtctca gggatcacct tgatcttaag agctttattt ggtattgatc
    58441 cagcttatac gactttatta atcaacatcc cattgatttt actaggagga aagatcctcg
    58501 gtaaacgttc cttggcctat actgttctag gtacactttc tttgtcattc tttttatgga
    58561 tctggcaaag ggttccacta gaaatcaatt tacagcatga tctactgatc gtttctctac
    58621 ttgcgggttt agttgcaggt gtaggaagcg ggatcgtcta ccgcatgggt ggaacaacag
    58681 gcggcagtga tattatcgct cggattttag aacaaaatta cggcatcact atggggcgtt
    58741 ccctattggc atttgatatc atcgtcttac ttgcttcatt aacctatatc gacttgaaac
    58801 gtatgatgta tacgttgatt gtttcgtatg ttttcagtcg tgtgattgat tcgatcttag
    58861 atggcggata ttctgcaaaa ggtctacttg tcgtctccaa caaaagcgaa gagattgcac
    58921 cactactgat gaccggtctg caaagaggtg tgactttcct ttctggagaa ggcgcattct
    58981 ctggtaattc aaaaaaaatc atctatatgg tcgttagctc tagtgaatta actgaagcta
    59041 agaggatcgt ccacgagatt gacgaaaaag ctttcctttc aatcatcaat gtgcatgaag
    59101 tggaagggca aggctttact tatttgaagc cacagacaag attactgaaa aaactataag
    59161 aaaaaagaca aagataggct atgaggaaag attccacatc cagctgacct ttgtcttttt
    59221 tagtactata ctatttttaa ctaattgtta tgttcactta cccggttact tttcttttgt
    59281 gtctgcaaaa ggatctttcg gttaacttca tcagataatt tcaaaaaagc atccctatct
    59341 ccatcttcta gtgcttgatc gatttgagca taaagcattt ttagttttgc ctcttcttct
    59401 ttttcttgaa aatatcgttc aattaattca gtatcttctt cactgactga agaatcccaa
    59461 gaagaaaatg gattgtcttc taagatagat agatattctc ttgtctgcca agcattttcg
    59521 aatatacatt ccacatataa aggctttttc caattcaaac ggatttcatg aaaaatctgg
    59581 tctgtgtccg taaattcttt tccctgcaaa aacaatttca tgggttcccc ttcaacagat
    59641 agatcactga tctgcagtcc tcgatccgca aaattcgccc cttcaacaaa gtggacattg
    59701 cttaaaatcg cttcatgatt ggcaagataa ttcaagatcc aaaaaacttc tcttcggcta
    59761 aaagatacat gattgaccat ccacgttaaa aaattcttct tttcccgtac atcaattagc
    59821 atttttctca cctctctgtt aggtcatcta ataatgacag tgcctccata tctcctggtt
    59881 caagtgttaa ataataagtc aataattctt gcgctctttt cagctgccct tcttctctta
    59941 agaaaaaggc atactctttc aagaaatctg gttcatgtga tagttcatga taagcctgtt
    60001 catagtgtac agctgcaacg gtaaagtctt ctaattcatt ataagcatgg gctaggttcc
    60061 attctgcata aggattagca gtttcttcca tctgattgat cacttgtatc acatcttcat
    60121 atctttcatc atccaagtaa agattactta aagtaagtaa tgtttcatct tgtttgtctc
    60181 ctacttctaa agctttcagt aaaagttctt ctgctcgctg tctatcatgc agacggaaag
    60241 cattttccga ggccaggtga taaagttcga cttggtaagg attttctttg attccttctt
    60301 ctaacactgt ttgtgcttct tcaatcattt cttcttcttg aagagcttgt cctagatata
    60361 gatataacga ttgataatgc ggattaactt ctcgaagttc ttccagatag ttgattgcct
    60421 gctgattttc tttcaattga agattcgtaa aagctagctg gaacagtcga tcatctgtcc
    60481 tttcttcttt cagtgcttct tctaaaggta cgatagctgc ttcgaattct ccttgcatac
    60541 ttaaggattg tcctaaacgt tcctgcatcg agattcctga aatttcattt atcccgcttg
    60601 ccaacaatgt ttcataaacc actgctgcct ctttgaaacg atcaactgaa aaatacaatt
    60661 cagctagcgc aaactggatc aatggttcat caggaagcaa cccagctgct tctttcaatt
    60721 ttgcttcact cacttcaggg attccgataa cttggtacaa atcagctatc gccaataagc
    60781 tttgcggata gtaatcactt tgcttatcca ctttttccag gtattcaaaa gctaagtcga
    60841 tctcattatc ttcgattgca atttctgcta acgggatgtt cagtccgtca ttttctgggt
    60901 attggttgat cagttgttca aaaatctgtt ttgcttcgat caaaaagcca atagccagca
    60961 attcttcgcc tagttcagct aatatatctg ctgggtcttc ttttaatgct ttttctaaaa
    61021 agatttgtgc ttgtgacaag tcttcatctg ataaagcttg caacattttt tcgctatttg
    61081 tattcatttc aattccctct tatctcactg acgatttgtt gaaataccgt cattgttctc
    61141 aataattctt tttcgtttgg atgttggtat ccgtctatta tacctatttt ttctaaaaca
    61201 agtatttttt tgcttttttc tgccaaaaaa agaacttttt ccaaatactc catctttgaa
    61261 atctgttcca gccagtggat tggatagcca agcaagtcca gccacacaaa aaagtttttt
    61321 agttggaaat caaaaggata tgcttggaga ttccacacga aatgaaacat cattcctaaa
    61381 aattttttca tactagcaga aagtagatgg ccattttcgg tcagataaaa agcttgttca
    61441 aacagtttac cataatttga caggttttct gcctctaatt gataaaaata gatcatttct
    61501 tcgatcatcc cggcaaacgg ccgagtcgtt aattgctttt tatttggata gtttttatac
    61561 agattttgca aaaaacgatg atcacaaatc attccgcaac agataaaagt caaaaagtca
    61621 atctgctttc cgtccacctg ccgctcgctg attgtttgat cataaacgat gcgttctggc
    61681 agattgatgg tttgcagcac tacacgattt ggtagctgca aaatcgtccg ctgcggtgtt
    61741 aatgctcggc ttagagaacg gatcgataca gggatcatcc atagtgatga atcaagggtt
    61801 gtatgttttt gaaaaaaacc tgccagatcc atgatccctt catttccaaa tccgatgata
    61861 aacatggatt gattttcggg atagcgtttg acgaaatcaa gcaggttctt taattctgtt
    61921 aaatgattgc attgagtgtt ggcacaaata taccagtcat tgtttgcttg atttgcaaat
    61981 aacgggttga tcttatctgc aaacaaatca taatagcgct gattcgttaa aaataagatt
    62041 ggctgatcaa tggaaagttc tcttagctga ctgactagcg tttctccgta aaacactttc
    62101 gtcaacgcat cttctacttg ataatcaagt tccatacgca tccctcctct tcttattttt
    62161 attttaccat tagtaagcaa attctagaaa agataagagc aaagctcacc tcataacttg
    62221 caaataaata aaaattaaat aaaatacatg tgttttctat gtatgacata cggattcgga
    62281 atctctgtct cttatttatt ccttttctgc taggaaataa aaagaaaagc acttgtagcc
    62341 ctagagccac aagcgctttt tcaactgtct tatttaacag cgtcttttaa tgctttacct
    62401 ggtttgaatg caggtacttt gctagctgga atttcgattt cttcaccagt ttgtgggtta
    62461 cgacctttgc gagctgcgcg ttcgcgaact tcaaagttac caaaaccgat taattgaact
    62521 ttttcgccgt tagctaaagc atcttgaata gttgaaaata cagcatctac tgctgcagtt
    62581 gcatcttttt tagttaaatc agtagctgaa gcaacttttt cgattaattc tgctttgttt
    62641 gccatggata gattcacctc ctccacaaga gatatgatag aaatatcatt tagttaaaac
    62701 tttctgagtg gtccagaagt ttaaaaagaa atattgatta acttcaatat tctgttatca
    62761 cgatatcata tgaaccgctt aatagcaagg tttttaccaa ttttcacatg ctttttttag
    62821 tcatagaaac gtaacaaacg aaactgtctt tttcttttga aaaaaaaacg tcgatatggt
    62881 aaaatgccta ttttctccta cgagggataa ttcggatgga cgtcccttca aaagtaaatg
    62941 cttttctgat ttgattttct aagaaacgtt cataagaaaa atgcatcaat tcctcttcat
    63001 tgacaaaaac gacaaatgta ggtggtttga cagcgacttg tgtagcataa aaaatcttca
    63061 ggcgttttcc tttatctgtt ggtgttggat tgattgctac agcatccata atgatatcat
    63121 tcaacacagc cgaagagata cgaagattct ggttcatact tacttgttcg atcatttcag
    63181 gcagacgttc caaccgttgt ttagtcactg cagaaacaaa aacgattgga gcgtaatcaa
    63241 gataccggaa ttcatcacga atctcttgtt cgaaatcacg cattgtattg gtttctttct
    63301 ttacaagatc ccatttattc acgacgataa taattccacg accagcttca tgggcgtaac
    63361 ccgctactcg cttgtcttgt tcccgaatcc cttcttcagc attcagtacc atcaaaacga
    63421 tatcagaacg gtcaatggca cgcattgcac gcatcacgct gtatttttca gtattttcgt
    63481 atacttttcc gcgtttacgc ataccagctg tatctatcat taagaatttc tgaccacttt
    63541 cagaaacgaa gtgggtgtcg attgcgtctc gggtcgttcc ttcaatatcc gaaacaatta
    63601 cacgttcttc tcctaaaatc gcattgatca aagaggattt accaacattt gggcgaccaa
    63661 tcaaactaaa cttaatcgtg tcgtcgtcct cttcttcagt ctcttcagag aaatacttca
    63721 ctgcttcatc taaaatatca ccaatcccta gtccatggct tcctgaaaca ggataaggat
    63781 cgcctagccc taaggaataa aattcataga tatcgtttct catttctgga ttatctactt
    63841 tattgactgc taaaatcact ggtttattac ttctgtataa aattttcgca accatctcat
    63901 ctgcgtctgt cacgccttcg cgtccgctga ctacacaaat gattacatct gcttcttcaa
    63961 tcgcgatttc tgcttgatgc ttgatctgat ccataaaagg ttcatcgcca agatcaattc
    64021 cgcctgtgtc gatcacgctg aattctcgac ctaaccattc accttttgca taaatacgat
    64081 cacgagttac tccaggagta tcttcgacaa tcgagatacg ctcaccagcg atgcggttaa
    64141 aaatcgtaga cttaccgacg ttcgggcggc caacgattgc aattgttgga tttgccatat
    64201 tatttcctcc tcacaagttt ccaatctcac ttagtttaac gaatgcattg ccaactttca
    64261 agcatagatt gtcaattaaa cgaagtattt ttattcctta ttgaaatact aatcgctttt
    64321 caaaagaaaa actgagacaa aagcagctgc tttcatctca gtcacagata caaaatcaat
    64381 tatttttcag aatcatcggt tgctttttct tctacttctt ctttcaacgc atcacctaaa
    64441 atgtctccca tagtaaatcc agtattttct tctggtaatt cataagactc aacatttttt
    64501 ggttcttctt ctgaagctgg tttttcttct aaagctttga tactcaatgc aatacgatgt
    64561 tcttctggat ggatctctaa tactttcact ttcacttcgt caccttcatg aagcacttca
    64621 tgtggtgtcg caatatgttt atgtgaaatt tgagagatat ggactagccc ttctactcca
    64681 gggaacactt caacaaaggc accgaagctt gtcaaacgtt taactgttcc gtctaatact
    64741 gtgccaactg gagcttttgt atcgatttct gaccaaggtc caggctgtgt gtctttgatt
    64801 gacaaggaaa cacgttcttg ttcaggattg atagacaaca ctttgacatt tacatcatca
    64861 ttgacagcca atacatcact tggttttgct acatgactat gtgaaatctc tgagacatgg
    64921 actagaccgt caattccgcc taggtcgaca aatgcgccaa aatcagtaag acgtgcaaca
    64981 cgtccactga gaacatctcc ctcatggata gttgaaagaa tttctttttt cttttcttct
    65041 ctttcgtttt ccactaccgc tttatgcgat aagatcaaac gattttctga tggttcgatt
    65101 tcgatgattt taaagctcaa tgttttgcct ttatagtctg aaaagtctgc aacaaaatga
    65161 tcttccacca ttgatgcagg gacaaaacca cgtacaccga cgtcaacgac taaaccgcct
    65221 ttgacaacat ttgtaacagg tgcttcgatg accttgccct cttggaaatc tctttcgata
    65281 tcttcccaaa cttttttagc atctaaacgg cgttttgaaa gtagatagct gccgttttct
    65341 ttatctttgc cgatcgtact gataacgact aaatctaata catcaccgac ttttactact
    65401 tcgctgatat cttctaccgg caaagttgat aattcttttg ccggaacgac tccttcaact
    65461 ccagcccctt cgattccgac aatcacttgt ttgtcttcaa tgactaaaac ttcgccctta
    65521 acaacatcgc caacttttac ttcttgtacg cttttcattg cgtcttccat tgactggttg
    65581 ttctcttgat tttgatcaaa ttctgtcata acacttgtcc tcctaaccaa cattctgccg
    65641 taagtacggt actgttttta gtttaattga ttattcaaaa aaaataaagc gatttcgttc
    65701 gttttccttg aaaaaacaat tgtttttcat tttttttctt aatttaaaaa agaagataac
    65761 cttttttcaa aacaacagct tttattgctg caacgacttc tggaatcgat ttgccagtcg
    65821 tatcgatacg aacagcatcc tctgcttgct tcagcggaga gacgtccctt gttgaatcat
    65881 aatggtctct ttcctgtatc tctttcgtca gtgtttcaag atcagtcgga attccttttt
    65941 cttggttctc tttgaaacgt ctttctgcac gctcacttac gcttgctact aaaaagattt
    66001 tcacttctgc attcggtaat acagctgtcc cgatgtctct tccgtccatg acaacgccac
    66061 cgcgttcccc tatttttcgt tggacagcaa ctaattcttc tctgactttt ccatgtgcag
    66121 agacttcaga aacagctttt gtgacatctg gttgacggat ttctaaagtc acatcttttg
    66181 atgctatgaa tacatgctgt ccatcttctt gttgcgaaaa agagatagga tactttttga
    66241 tcagcgaaac tagcccttct tcatctgaaa aagatacatt gttcgtaata gctaaataag
    66301 ttactgcgcg atacatggca ccggtatctg tataaatata attcaattct tttgctaata
    66361 tcttggcaac ggtacttttc ccagatgaag caggcccgtc gattgcaata ctgatttgtg
    66421 tcatgatata actccttttt ctcagaaaaa aagccgcaaa gcgcggctat tattatttaa
    66481 ttcttaattc ttgaccaggg taaagcataa agttgtttgg atctaagcca ttcaattcaa
    66541 acaattgatc agtggtgatc cctgcacgct cagccacttg ttttggacct tcgcctgctt
    66601 gaacagtcgt tgttgaaccg gctgcagctg atgaagaagg tgttgtttct gctgcattgc
    66661 tgcttgaatc ccctgcttga taagttgcct cacttgagga agtcggtgtt gtctctgctg
    66721 caatactgct ttctgacgtt acacctgtat cttgagacac agtagattct tccgcgattg
    66781 tactagattc tactgttgaa gtctcagttg actcctcttt tgttgtactt gtagaacttt
    66841 ctactactga tgtcgaagtt tcagaagaag ttgctgctgt tgcattgttg cttgatccat
    66901 ttctgatcca gaaataagca cctgctggaa tagcgataca taagaccagt aaaatcaaaa
    66961 atagcgaaag aaataatgta tttcctttct tttgttgtct ctgagaactt cttgatgaaa
    67021 cttcatcatc tgtatcgtag attggttgtt cccatggttc ttgtgtttcg ttattttctg
    67081 tagattgatg tctatctttt ctgctcactg ttttacctcc taaactctat cgtgtgtatt
    67141 ataccatagg aaaaagtgtc ttgtcctaaa aaatcaatgt tccatgtttt ttttaaataa
    67201 atttagtaaa atcgttcccc aatgaagttg ttcagtctta gaaaaaagct tcctctttgg
    67261 cagaagttct gcttttgctc catcaatatc acaacaattc tgcggctttt cggagagttt
    67321 ttcgccaaaa taagctaaga tgaattttct gcggcagttt tcttcattaa tatagcgaag
    67381 catctgctgc aggcggaatt ctttttcttt ttcctgacgt ttgagccgtt ccacccaatt
    67441 ttctggtttt tcagactgct ggatcagctc catccatttt ttttgcagct catcaaaggg
    67501 agcttgctct gtggcatatt ccaaataaag ctcaaaactt tgtcgctgtt ctctagaaag
    67561 ttgattaaag aaataatgga tacgttcatc cccactttga taaaaaagaa ttgctgcact
    67621 ttcttcctga tctctgcctg ctcgaccgat ttcttgtaca tagttttcta gactgtctgg
    67681 caaatcataa tggaccacat aacggatatc agatttatcg atccccatac caaatgcatt
    67741 cgtagcaatg agaaactgca gctgattttt tacaaactgc tgttgaagtt gtcttctttg
    67801 agctgcatca agaccaccgt gatagtatcc aactgtaaaa cgttccctga ataaatgata
    67861 caatcgttcg acttcttttt ttgttgcaca ataaatgatt gccgctcctt gtgctctttc
    67921 catgaagtgt tcaagttctt gttctttttg ctgcgttttc cgaacaaaaa gcgagatatt
    67981 ttgccgattg actgattgtc tgatctcttt tggtggtcga tcaaatagta catgacgtat
    68041 atctcttgct actaaatctg ttgctgtcgc cgttagtgcc agtgtgaccg ggtttcctaa
    68101 aagctttcgg atttttccta actgttgata ttctggacgg aaatcaactc cccattgaga
    68161 cacgcaatga gcctcatcta ctacatataa cgcaatattt tgttttttca gatgctctaa
    68221 aagagaagga ttagtcaaca tttcgggact caaaaacaaa aatttatatt gcgatagatg
    68281 acggaggacg tatcttcttt cagattgcga aagtaaacta ttgaaagcag ctactcgttt
    68341 ctcccctcgt ttttgtaatt gcgtcacttg atcttccatt agagaaagca gcggtgacac
    68401 gatgatgacc agaccttcca ttaaataccc cgtcaattga tagcaaaggc tttttcccgt
    68461 tcctgtaggc aaaatagcga gagtatcttc tccatcaagc aacgcctgaa taatttcttc
    68521 ttggccggga cgaaatgaat caaatccaaa atattttttt aattcattta ttaacattct
    68581 gtatcgcttc ctttctcaat agatagattt gatacaaacg aaattctcca taatctaatt
    68641 gtccatctac attccattcg ctaaagcgcc actctcgcac ggaatcctta tttggttcca
    68701 aacgttcgat tgtttctgca gagagaatcc actcgtatgg aaaatcatca aaaaacaagg
    68761 cccactcaat aagatgatct gttactgttc ctctttttaa atgacggatt gccatgatct
    68821 ggtcaattgt ctgaccattc ataaacatct gcttggtgat catcatgctt tgattgaaat
    68881 tttgctgtaa taatgaatcg atcaaagcgt gccaaagcga atctggccgt ttttcgattt
    68941 gtgctaacaa aaggtggata catctgcttt gttgcagata aaccgctgac tcatcattgt
    69001 tttttgccag ctgataagga agcagccctg ttcgctcatt tccagataat tggttagcta
    69061 aaaaatctgc tgcttcttct ggtatctccg aaaacagctg caccaaactc tcatacgcag
    69121 tatcaatcaa aatttcacgt ggcaattggc tttcactaag ccattttttt agttggaaag
    69181 tataaaaagg acttgtctct gctggaagat agtcttgaat acccgttgcc agattagaaa
    69241 ttacttgaac agcaaattta atgagccgcc aactagtttc ccctgttctg ccatagcggt
    69301 caaaacgcaa accagtatgt tctatttgat gttcggacaa catatcagcc cctgcaggcg
    69361 ttagctttgc ttcattttca tttatttcga tccaaccttg ctgcgcaatt ttttgcaaaa
    69421 tttgataaaa cttatcctgc ttcaattctg gaaaactgtt atggataaac aaaagttcgt
    69481 gggtaaatcc ataaataagt actgaagtcg ttcgttttcc cacaaggagt tggtaaagag
    69541 aactaatccg cagcttgtgt cctgatgaaa atagcgataa aataaaaaca atcatattat
    69601 tctccttaag ttaggatggt tagaaatgtc actactatgt aaacttgtac ctgaacgctg
    69661 tattgcatgc ggtctttgtc aaatcgaagc gccagatatt ttcgattatg atgaagaagg
    69721 aatcgtttta tttgcccaag aaccagaggc tcatcaacag ttcgtgatgc cggaagaaga
    69781 aacatatgta aaaaaagcgt atcaaaaatg tccagttcga gcgattttat tagaaaataa
    69841 aatataaata taaacgaaac tttaaaaacg tacactaagt tctctaaact tgaaaataag
    69901 ccgggatctg tttctcttgt tccgtgaaat cccgacttat tttgaaagat tcatttgcta
    69961 tctatagtta attgactagt tttttcacgg accctatagg acccatttat attatttcaa
    70021 ctttttcagc cagttttcca atgtattgac gatcacagtt tgctggtctg cgttagaaat
    70081 ttctgcctct ccatctcctt tttgctcacc atagctgcca aaacctccat gatttccgcc
    70141 attgattgat acatactctg ttctttccgg taaatactgc ttagcttctt gataagcttc
    70201 ctgattcaat actttatctt ttgtcgccgt gatcgataaa acaggaacag ctgattctgt
    70261 caatgttcct tttttgtcgg gataacttgc catgaagaat acccctttta aatgtttgtc
    70321 tgaatgattc ttattgacat aacgacttgc cattacgcca cctaatgaat ggccaccgat
    70381 cacataatcg gaatttggaa aatcttcttt gaccttatcc gctttgtttc cgcccatcac
    70441 tgctagatca agtggcattt tgacaatagc gactgaaaag ccgttttttg ctaaatcttg
    70501 cgcccaaata ctataacttt ctggctctac gagagcacca ggataaaaaa taagtaatgg
    70561 agactttcca tgattctttt tctctccttc aaaaaataag taatcttctt tgttttttcc
    70621 ttcttttacc aacacttgcg cttctgacga tggttcgtac gtattttgct gtatatagta
    70681 ataaccggct ccaccgagta atcctaaaat gatgactaag ccaattagta ttcgtctaaa
    70741 tattttcact ttctcccacc ttttctatct gagtgacccc tttttcttaa tcaagtatga
    70801 ttatacctta aaaaaataag agagaaatgc aaactttcca gcatcaaata aaaaaagacg
    70861 gaatatccat ttgaatatcc gccctttttg tatgtttatt tttatttagc acataaacga
    70921 tacacagctt ctgcataaat ttccattgct ttatacatac tacttaattc ccatcgttca
    70981 ttcgcctggt gcataaagtc aggagtatca gggaacatcg caccgaatgc cacacactgg
    71041 ttcatcgtac gagcaaaagt agcgccgcct gatacaaacg gttcagtcat gtctccagta
    71101 atatcgcgat atgtccccaa tagatttttc actaactcac tgtctagtgg cacgtataat
    71161 gaatcaagga agtcaaattc ttcataagtc aacccatatt tttctgctgt tttagttaat
    71221 ttagctacta aatcatcttt cttgaatgta acagggattc tcatatctac accgattttt
    71281 gtttgttctg gtgtgatttc caaacttgca aagttcatcg ttagctcgcc ggattgttca
    71341 tcttctgttt tgcccacgac agcttcacca gttgcatttt cttgaactaa ttttcctaag
    71401 aaattgattg ggccaaaatc aaatacttct gaaagagcaa ttgccagacg agaaatagca
    71461 ttcactcctt gtgctgcatc tttcgcgtgg atacttttac caagaacgac gatgccttca
    71521 ccttgttctt cgaaatcgaa ttcgtgtttt ttcaatgctt cttttacttc agaaagcttt
    71581 tctccagagt aaggagctgc atcaggaaca acattcaacg ctcctccagc ttttacagaa
    71641 aactcgttcg ttcctggtcc tgtcaagtaa gcttgcaata agcctttttc tgcataaatt
    71701 aaaggaaatt cagcatctgg cgcaaatcct tgcgtaattc cttcttcttt ttcgttatat
    71761 ttttctaaac aacgccataa attttcttca tctgtaccaa agataaaacg aatacgtgtc
    71821 ttgaattcca cgccagcatc aatcaaagct ttgacggcat acattgccgc aatagaagga
    71881 cctttgtcat cttgggatcc gcgaccaact aaccagccgt cttttatagt tggatcgaat
    71941 ggttttgttt cccaattatt ttcgtcccct gctggaacaa cgtccatatg acacaagata
    72001 ccgaataatt catctccaga gccaatctct gaataaccgt aatagccttc tggatcttta
    72061 aatgttctaa atcctagctt ttcactgatt tccaatactt tatccaaagc ttcaatcact
    72121 tttttaccga agggatgtcc ttgtcctgaa tctgcttcat ctagtacgga aggaattcgg
    72181 atcaattcgc tcaatgattc taatgcttgt tcatgatgtt gttctgtcac aaattttttc
    72241 atgatattat cctcctaata tgttaaaaag ggtcatgtca aattcctccg acacaacctc
    72301 ttcttctcaa ataatgacgt tttaagcctg ctccatcagc aagcagaatg ttcttaacaa
    72361 atgatgaaag aaaaaacact cactgaatgg agctattgac ttatccacgt gcttatcgat
    72421 agccgtttat cggtttcttc attcttatga tagaccttgt acctgtattt gtctaggttt
    72481 agaacattgc tgctacacct aggatcacac agatggctac aaataatatc gcaattaatt
    72541 taccggtaaa tttccaccac actgaaacat cgaaacgagc aattgctaca gcacccatta
    72601 cgatagcaga tgttggtgta attaagttta ctaaaccaga tgcagattga taagcagtga
    72661 ttactaaatc cttaccgacg ccggcaaatt cacccatagg tcccatgatt cccattgttg
    72721 cagcagctaa gccggaagtt gaaggaatca agaatgacat agggatatag aaaataaatg
    72781 taataatgat gaatacactt gaagataatg aacttaatcc ttgttcgccc caatgtaaga
    72841 tggtgtctgt gattaatcca ttgttcataa ccacttggat accacgagca acagctacta
    72901 cgattgctac accgatcaaa tcagaagcac cagctaaaaa ttcagagaca aattcagact
    72961 ctttcatgcc ataagtgatt gctactacga tggacattac taagaataac atagtaattt
    73021 ctgggaagta ccagtcacct aatggaagca tgcttttacc tagaaccgta cctagtaccg
    73081 gaaggtttgt taaccagttt gtcaatgatt caaaaatcgt gaaattcgca ttcaaggttg
    73141 tccaaggaat caaactgatg atcatgatac caaaagtaat aaagaacaat acattaacat
    73201 gtttttgttt tttcgtgatt acttcttgac gaccgattgc ttcaatatcg aaatgcttca
    73261 tatcttcttt acgttggttg tatactaatg attttgttgg atctttttcg atctttgatg
    73321 cataacgata cacatataca atagaaatag ctaacaaaat aacgaacatc aagaatcgaa
    73381 gaccaatacc ttcacccatc gaaatcccaa cagcttgtga agctacgcct gttgcaaaag
    73441 gatttaccgt tgaagctaga cagcctactt gagaccctac taatacgatt gctacagcta
    73501 ctactgtatc aaagcctaca gccatcatga ctgggatcag taaagcatag aatggaatcg
    73561 tttcttctgc cattccgtaa gtagagccgc ctaatgcaaa taacagcatc aatactggaa
    73621 taagcatttt ttcttttcct ttgaatttct tcacaactga agcaatcccg gcatctagtg
    73681 cccctgtttt gttgataata cctaaaaatc caccaacaac caaaatgaag aaagagattg
    73741 ggattgcagc aggtgtttct ttagttagct cccccgctgg tacacctagc atcccattga
    73801 ttggcgccat gaaaatatcc cacagccctt gaggattttg ttgtactcgt tcatatgatc
    73861 cagcgacgat gttgcctgca tcgtctacac tatagtgccc agctggtata aaccaagtaa
    73921 gtacagcgat tactgcaata ataataaata agaccgtata cgctgaaggc atttgaaatt
    73981 ttttcttttt tgtttgttcc atcttcttct tcctttcttt tccgttagag acaacaattt
    74041 tggctctaat gttattttat caaaaagagt atcagaacaa aaataataac acgcattttg
    74101 taaaactttt gttcaaaaag ttcgtttttc cctattccct cctctttttt caggttttta
    74161 aagctttctt ctttttcatt gtagcgtttc ctttttttta taaacaaaca ttttcgtctt
    74221 ttaaaaggag aagaatttca catttgcaaa gaattgattg atttttatgc ataaatcaaa
    74281 aaaatactca aaacaaacaa ataaaacgtt tacattattg catgtcttgt atttttttcc
    74341 aaaaaaaaag accaagaaat ttcttgatct ttttgatatt tatttgactg ctgtatgacg
    74401 ttgttcgaac tgatgttgtt ttcgacttaa ccatggaaga agttgcgcat gcagcactaa
    74461 aaagacagca ctcacgatga atcctttgat caagttgaaa ggaacgatcc caatcagtac
    74521 atagtttgct aaagacatgc ccagcatctg tgttgcgttc agccctaaaa acattaagta
    74581 aagaggcgta ataacgaaat aatttgcgat actcataaaa atagttagag cgatggttcc
    74641 tgtaatgaca cctaaaaatt tgtttcttcc tttatgctgt ttctttacac taaagaaata
    74701 ataaatcggg aatgtaaaga tcgttgttga taagaaacta gcagtgtctc ctaccaaatt
    74761 atccggagaa aaacctgtag tcagcagatg taatactgaa cgcaagaacg ctgttgcaac
    74821 tccggccgct ggaccgaata agaacatgct gatcaatacc gagatatcac taaaatcgat
    74881 cttcaaaaaa ctaaaagccg gcatgatcgg aaaagcaaaa tactgtaaaa ctaatgcgat
    74941 cgcagcaaac atcgctactg caaccatttt ctgtacacgg gtgttcttca tttgaaatcc
    75001 tcctgttagg actcctcttc agcgaacttt ttgacgtttc tctccaaatg aaaaaactct
    75061 attttccagg gaaaatagag ttagagttat ttgatcaagc gtcgtcaaat aagctctatc
    75121 ttctttatcc agactatact gtcggtatcg gaatttcacc gattcagcta tttttttgca
    75181 aaataggtcg tggactataa ccaccggtcg ggaatttcac cctgcccctg aagacgaacc
    75241 ttatataatt tttgtttaca tattcattat acatatctat tcaaaaaacg caacatctaa
    75301 cacttataaa aagtaagtgt tcattttgag tggttcagca aactgctgac ttcttttttg
    75361 ttcagttcac ggtaatcccc aggacgtagg ccttgtaaag tcagatctcc gtatttttca
    75421 cgttttagtt tttgcacagg aagaccaact gcttgaagca tatttttcac ttgatgatta
    75481 cgtccttcgt ggatcgtcaa ttccacgata ctgtggtttg tcttaggatc cactgataaa
    75541 atttggaatt tagctggaga tgtttttcgt ccttcgatct ttattccttt tgtcaaaggt
    75601 gcaagcatcg attttgtagc gatccctttt acctttgcaa cgtaaacttt atccacttca
    75661 tgttttggat gtgtcaggcg ctgagcaaaa tctccatcat ttgtcagtag caagatcccc
    75721 gaagtatcgt aatccagtcg acctacagga tagatacgtt cttttaccat cggcaaaaga
    75781 tccgtaacaa cttttcttcc tttgtcatct gcaacagcag aaataactcc tttaggtttg
    75841 tacaataaat agtaaccgta ttcttcttga taaattggga cttcatccac ttccacagtg
    75901 tcatgtttcc caacttttat ccctaattcc cttacaactt gtccgtttac ttttacacgt
    75961 cctgcaagga tgaattcttc tgcttttcga cgagaagtga tgccagcgtg ggcaattact
    76021 ttctgcaatc tctccattac aattattctc ctttattcat tccatcaaag aatagatctg
    76081 gtgacacgtc ttcttcaagt gaaatttcca tttcttgtgt atcaggcagt tcttctaatg
    76141 atttcaaacc aaaataatct aagaagtagc cagttgttcc atataatatc ggccgacctg
    76201 gtccttctac tctacctttt tcttcaatca gtttatgggc agctagacgc tgtaccgctc
    76261 ctgaaacctg aactcctcga atctgctcga tttcagctct tgtgattggc tgtttataag
    76321 cgatgattgc taaggtttcg acggcggctt gagataatgt gtttgatgtc ccttgcgcat
    76381 atcgcttaag taagggagcc agctcttttt tagtcgttaa tacataatgg ctttccgttt
    76441 caaaaatatg caaagctgag tgcgagtttt gagtgtaaga ttcttttaat tctttcacta
    76501 aacttgtgac ttctgcttcg ctgagattta acagtccgct taattcttca ttactgatcc
    76561 cttcctcgcc aacaataaaa agaagggctt caagttcact taatttcgtc atgttcggta
    76621 tctcctactg ctggataaag cataattgat tcatagtttt ctttttgttg cgcaatgatc
    76681 tgcccttttt tcatcaattc tagcaaagcc ataaaagtag tgacaacttc acttttagaa
    76741 ggtgactcaa aaaaagaatc gaaagtacag ccatgcgcat ttctttttat acgctcttcg
    76801 ataaatacca ttcgttcttc gatcgttgtg tcatcgggcg tgatcgtttt ttccactttt
    76861 tgagaaaatt tcttacgttc taagattttc tgcatggcac ggatcaaatc gatttttttg
    76921 acttgtcctc gcttcaacgg cttttcagca tccgctaacc catcgatgtt cataggttct
    76981 tttgtgtaat aaagacttcg ttcctctgct ttttctgtca gtctttcagc tgcatattta
    77041 tatttacggt attctaaaag ctgagtgacc aattcttctc gaggatcttc ttcccataat
    77101 tcttcatcgt ctgtgacgaa ttcttggaca ggaagcaatg tcttgctttt gattgccatc
    77161 aatgtagcag ccatcactaa gtattctcct gcaacagcta gctcgagtgt tttcattgta
    77221 tggatataat tcatatattg ctccgtgatc gtagcgatcg gtatatcata tatatcgatt
    77281 tccatttgct gaatcagatg aagcagcaga tcgagaggac cctcgaatac atctaattta
    77341 atattgatct ccgtcaactc attcactctt tcttcttttt ctttgctagc cattttccaa
    77401 tgtaaggctc tgtgatttca gttgcacgga tggagacatc cgggtacttt tcctgtaatt
    77461 catctaacat ttgaaagccc atattctcac cgcgataaga cggattcaaa gaaatgtcat
    77521 ggatgatcag ctgatcgttc gttgcccagt aaataccaat aatcccttga atgttggttg
    77581 aaccttctgg cgtaaagaaa aaaagttcat aatcgtcttc agattcgtac gtatcaatct
    77641 ccttcaacaa agaagtttta cctgtcagtt tcttatggaa actcaatagt cccatagcaa
    77701 ttttacggtt ttctttccgg taatgtgtta acatcttctc accttcctat gactaagccc
    77761 ttggaaaatg ctgcttataa acatccgtca ttcttttctt cgtgatatgt gtataaatct
    77821 gagtcgtaga gatatccgcg tgacctaaca attcctgtac tgtccgcaaa tcagctccat
    77881 tttctagcag atgtgtggca aagctatgcc gcagcgtatg tggtgttact tctttgtaaa
    77941 tcccagcctc tcgtactaac tgcttcagat ttttccagat tccttgccga gaaagacctt
    78001 tcccatgatt attgacaaat acatgcattt cagatgcgtc ctttacaagt aatggacgtg
    78061 cttcgtctaa gtagcgttcc agccattgga tcgcataatc acctaaagga atgatccgct
    78121 ctttatctcc ttttccaact gtctgtacca gtccaagaga aagatgaaga tctcccaatt
    78181 tcaatccaat caattcactg acgcgcatcc cagtagcata catgacttcc aagattgcac
    78241 gatctcttat ccctaacgtt tttgtcgtat caggtgcttc aatcagcttt tctacttctg
    78301 tcagagtcaa tgtacttggt aatttctgtg ctttcttcgg cgtatcgata tgttgcatcg
    78361 gatcatgatc agtgatacgc tcttgccgta aaaactgatg gaatcttctg agacttgaga
    78421 tcatccgagt aacagttgct gtcgcttttt tttcttcatg caacttctcc agaaaacgga
    78481 tgactaaaaa gcgatcgaca tcctgccatg aagcaatttt ctgctcctgt agaaaatgca
    78541 agtactgttc caaatcacgt tcatagcttt gtcgtgtatt ggcggacaat ccccgttcaa
    78601 tattcaagta gtggagataa tcgttgattt gctctttcat aataatcccc ctcatgcctc
    78661 tctatcatat cagataccgc ttcattctga tagtcttttt ccataatctg agcaaag
//
LOCUS       DASHHB010000005        78084 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.005, whole genome shotgun sequence.
ACCESSION   DASHHB010000005 DASHHB010000000
VERSION     DASHHB010000005.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 78084)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 78084)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..78084
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.005"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..2327)
                     /gene="esp"
                     /locus_tag="U2K71_000378"
     CDS             complement(<1..2327)
                     /gene="esp"
                     /locus_tag="U2K71_000378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="repetitive surface protein Esp"
                     /protein_id="HEM7314497.1"
                     /translation="MVSKNNKRVFLEKTKKRVLKYSIKKLSVGVASVLIGVGLVLGTT
                     ELVKAQDEISPSSPLETAISSVQVGDKVASGNTFQENPGYTKNYNFSDLQFSPQELTG
                     DTLKGNTIGFEVYGKHNIAASTKNWEIRLQLDERLAKYVEKIQVDPKKGIGSSRRTFV
                     RINDSLGRPTNIWKVNYIRASDGLFAGAETTDTQTAPNGVITFEKSLDEIFKEIGIDN
                     LKTDRLMYRIYLVSHQDDDKIVPGIDSTGYFLTDSDDFYNSLDVSENNPDQFKHGSVS
                     AKYEKPNTQTKDGSGSTGANGAIILDHKLTKNYNFSYSSSAKGTPWYANYKIDERLVP
                     YVAGIQMHMVQADKVTYDVSFESGTKVADLAIERREGHENYGIGSITNNDLTKLIDFA
                     NASPRPVVIRYVLQLTKPLDEILEDMKATAQVEENKPFGEDFIFDSWLSDTNKKLIQN
                     TYGTGYYYLQDIDGDGNPDDKEESGDTNPYIGKPELEEVYDVDTTVKGKVFIHELAGT
                     GHKAQLVDKEGTVLAEKTIAPNEKDGAPISDTVEFEFTGVDSSKLIAKDELKIQIVSP
                     GFDKPEEGSTVIKESPKAVDKQTVVVGFKPDAKESIRNNKNLPEDAEYSWKTEPDTSN
                     VTDSTKGIVTVKIGNRTFDVDVEFAVKASQAMENDATYVPITTTPETTVQSGKPTFDK
                     PDVPLAKDAFSISDVYNKYFGNASVDANTGIVTFTPAKGVGESEPITGTIPIKIVYQD
                     GSVGTTDLAVTVSKDIYENPGENIPAGYHKVTFTAG"
     gene            complement(2945..3379)
                     /locus_tag="U2K71_000379"
     CDS             complement(2945..3379)
                     /locus_tag="U2K71_000379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314498.1"
                     /translation="MRLYHLRSLEELRKLDFNKKFFWEISDLSCFVESERLSNGNNSV
                     LNEYDKVVINKVKSLCEEARESISHINGSNFKQTLFNLIKLDAKISSYLFFLIHDEFM
                     DYQKLDQLIERESWEDYYVGLTFSEKLNNYKLIDYKYLSESN"
     gene            complement(3396..4601)
                     /locus_tag="U2K71_000380"
     CDS             complement(3396..4601)
                     /locus_tag="U2K71_000380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YSIRK-targeted surface antigen transcriptional
                     regulator"
                     /protein_id="HEM7314499.1"
                     /translation="MFSDKEVLLKSLHVCLKLPIVVFNSEFSIIKEYRSDRTTYFFYD
                     YKKLLTDNIKKTKDFHFFTGDFNEMFLLYMFKTRYYLFGPFRANNIDKVFFKLKMNNL
                     NVAMADRERLYNSLQNLTLYSLGDIRDILILVHYFFTGKIEDLFHEPLIEYTENLSKT
                     IEQIKIDNLLSQNYDPEIYLFLYENKILEYVKNGDIRNLENMVFNLSNGIIPSVSGDT
                     IRSEKNYSIIVFEKLAQTSITLGMDIIEAYQSRDALIQENELAVSLPEVLKVRDSGIV
                     YYTKEIGKTKIEHLSPLISSVVQFIGLNIYKRITVKEIANYFSVSETKLRESFKNEMH
                     ITIYNYISKRKISTAKIMLKSNHTISEVSLGLGFSDSSHFSRVFKKYAGVSPKQYQLG
                     LVDNFNNIS"
     gene            5295..5681
                     /locus_tag="U2K71_000381"
     CDS             5295..5681
                     /locus_tag="U2K71_000381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM7314500.1"
                     /translation="MSDNDERVNAQLIRFFEKVILNTANTYYKKQEEIQKHEQLDQLP
                     PYLFTKDTIKFKQPINVLNVTLLVEDIKLAKILPLLKEPEQIFLIEKFIFDKTDKEIG
                     KVLGITRQGATNLKHRLYKKIQKMLV"
     gene            5775..5965
                     /locus_tag="U2K71_000382"
                     /pseudo
     CDS             5775..5965
                     /locus_tag="U2K71_000382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304825.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            6479..6811
                     /locus_tag="U2K71_000383"
     CDS             6479..6811
                     /locus_tag="U2K71_000383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM7314501.1"
                     /translation="MRKTYDRKFKLKISQDILEKKVTTKKIAEEYNISRPTISRWVSE
                     YRRYGKNAFAGQGKRLPDKADFYILEQENKRLKEEDECSTNTKIDTDLQGSLKNFTMK
                     ESKSINVL"
     gene            7107..7262
                     /locus_tag="U2K71_000384"
     CDS             7107..7262
                     /locus_tag="U2K71_000384"
                     /inference="COORDINATES: protein motif:HMM:NF012870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase family protein"
                     /protein_id="HEM7314502.1"
                     /translation="MPTVIHSDRGSQHRSFIYQELLNDHHITHSVSQTGTPVDNAVIE
                     AFHRSIK"
     gene            7538..8878
                     /locus_tag="U2K71_000385"
                     /pseudo
     CDS             7538..8878
                     /locus_tag="U2K71_000385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303667.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(9119..10735)
                     /locus_tag="U2K71_000386"
     CDS             complement(9119..10735)
                     /locus_tag="U2K71_000386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002406986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEM7314503.1"
                     /translation="MKKIPITILTGYLGSGKTILLNHILYGDHGKEIAVIVNDIGEVN
                     VDGVLVDKKGFKRTGEKLVSMPNGCICCTLREDLIVELDQLVQFSDLNQIVIEASGIS
                     KPIPIIQAIILAKNPLDIDLTQRLEIGAIIIVVDVKRFWDNFETGEKIQERKAEGNVV
                     EEEQDIRGLLIDQFECCNILLLNNSDLVSTKTIYKINGLIAKLQSNAKIIRTIKGEVD
                     LSKILNTNLFNIDETSYSVGWFKELELGYQNHIPETEEYGISSFIYRNRLLLDAQKFA
                     HLTDKNFPGNITRAKGFLWFAQHSDFSTIFETSGSYKSIESLNYWVASLPKIECQMII
                     QKNPELKEAWHPLFGERINKLVFIGMKMDQQCIKEQLEGCLTDSLNADNFRKISPFPW
                     KKSNMFSQISLMKFLRKAGVKIGDVIIIGILIITSFIPLFVFSIEKENETTEDAIKYA
                     IVRIDGKEMDRFDLDKINHKLVNYHPAKGQYNIVEIKDGRIRVKEDNSPDQIAVRTGW
                     ISKPGQTSICLPHKLVISIEKKESTDYYIY"
     gene            10853..11071
                     /locus_tag="U2K71_000387"
     CDS             10853..11071
                     /locus_tag="U2K71_000387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7314504.1"
                     /translation="MSQYRHLTILERERIFLLHEEGFSISIIGNDIQHSPFTILHELR
                     RNKKKTIHLPKRKKIMLIEEKNADVITF"
     gene            11266..11727
                     /locus_tag="U2K71_000388"
     CDS             11266..11727
                     /locus_tag="U2K71_000388"
                     /inference="COORDINATES: protein motif:HMM:NF033563.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM7314505.1"
                     /translation="MHHRGKTRHTKNHVESRGKICLTHSIHGRPKSADDRSDFGHWKA
                     DIVAGKTGGTCLVTLTDRKSCFLLAGKKVPRKKAAYVSEKMIELLSSIPNEAVKTITP
                     DRGKEFSNHFAVTEALNNMPFYFPDPHALGNNEQIKIRIDSFENTCQNRKR"
     gene            complement(11920..12159)
                     /locus_tag="U2K71_000389"
     CDS             complement(11920..12159)
                     /locus_tag="U2K71_000389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7314506.1"
                     /translation="MVRKVLLSMDSKNIDAYRRYRFAKQMRLNGSTLADIAATLHVSI
                     DTVHRVLKRDPDCCFIKVNTGRSRRLAKRHNSLAN"
     gene            complement(12183..13706)
                     /locus_tag="U2K71_000390"
     CDS             complement(12183..13706)
                     /locus_tag="U2K71_000390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEM7314507.1"
                     /translation="MKKISVGLIGIAALGLLGACSSTNDAKVSNDKDGKLEIVTTFYP
                     MYDFTKNIVGDEANVDLMVPAGSEPHDYEPSAKDMAKAHDADVFVYHNENMESWVPKA
                     KESWKKAGPNVVEGTKDMILLPGSEEEDHDHGEEDHHHELDPHTWVSPKMAIKEVSNI
                     KDQLVKLYPKKAKVFETNAEKYLTKLKRLDADYTTSLKEAKQKSFVTQHAAFGYLALD
                     YGLKQVPIAGLSPEEEPSSGRLAELKEYVKKNKINYIYFEKNANDKIAKTLANEAGIK
                     LEVLNPLESLTKEQMDNGEDYVSVMEDNLKALEKTTMVAGEEVVPEKEAKDEKTVANG
                     YFKDADVKDRELSDYTGEWQSVYPLLKDGILDEVFDYKAKINKDMTAAEYKDYYTTGY
                     KTDIDTINIKDNTIDFVVNGEHHQYTYKYVGYKILNYEKGNRGVRFNFETDDAGAGRF
                     KYIQFSDHGIAPSKAEHFHIFFGGESQEKLYNELHNWPTFYPASLSKHEIAQEMMAH"
     gene            complement(13724..13846)
                     /locus_tag="U2K71_000391"
     CDS             complement(13724..13846)
                     /locus_tag="U2K71_000391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEM7314508.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKEHKRNKMKKLA"
     gene            complement(13876..14859)
                     /locus_tag="U2K71_000392"
     CDS             complement(13876..14859)
                     /locus_tag="U2K71_000392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HEM7314509.1"
                     /translation="MGIPVTIVSGFLGAGKTTLINQALQVSPYPREEIIIIENEFGEV
                     GIDHKLLVHSEERVLQLNNGCMCCSLRSDLLAILISLLETIEEHQQPISQIIIETTGI
                     ADPQPIIQTLLTAPQLRGRVYIDSLLTVIDSDSFELIEREPQALKQLVMADRIFVSRK
                     QNLNLSALKKLQREIKSINPLSDIRMFTHSETIQKKDFYNLKKFNHPLVFESDSSENH
                     YNGKYYHQHHQVHEFKSILLTSDQDIKKDFLLQWIDWLILTNQGSLYRFKGLIALQEQ
                     DFIVAMQEVNEQVNFQYTTRQKDQTTIILIGKMLDEERIKQSFDKLLAESK"
     gene            complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="U2K71_000393"
     CDS             complement(14883..15032)
                     /gene="rpmG"
                     /locus_tag="U2K71_000393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM7314510.1"
                     /translation="MRQNIILECVETGERLYLTSKNKRNNPERLELKKYSPKLRRRAI
                     FKEVK"
     gene            complement(15053..15430)
                     /locus_tag="U2K71_000394"
     CDS             complement(15053..15430)
                     /locus_tag="U2K71_000394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314511.1"
                     /translation="MEIIYPPLVEEGLKYYLETTQQSLDKCTFYRSMVERGIITETGL
                     PTQQAIENGLVKDYYEDQGLSFDDFLRIYPIFEEYDEELFQCIDGYWEIPVDMKENLV
                     SQLESGELTFEDAQQIQAYLEDR"
     gene            complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="U2K71_000395"
     CDS             complement(15462..15641)
                     /gene="rpmF"
                     /locus_tag="U2K71_000395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018345355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEM7314512.1"
                     /translation="MAVPARKTSKAKKRLRRTHQTVVKPEISFDEANGDYRRSHHVSL
                     KGYYNGKKVIKGASK"
     gene            complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="U2K71_000396"
     CDS             complement(15656..15925)
                     /gene="rpsN"
                     /locus_tag="U2K71_000396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEM7314513.1"
                     /translation="MAKKSKIAKAKKQMAMIEKYADKRQELKAAGDRTALAKLPRDSN
                     PNRLRLRDQTDGRPRGYMRKFGMSRIKFRELAHQGLIPGVKKASW"
     gene            complement(16448..18214)
                     /locus_tag="U2K71_000397"
     CDS             complement(16448..18214)
                     /locus_tag="U2K71_000397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314514.1"
                     /translation="MQMIGGLTMKSFYKNKFALSDQGAEALTKASISSFFVYCINMVP
                     AFIIMMLIDELVLENTKPRWLYFAVSFVTLLFMYWLLDREYENLYNSTYKESAHLRVQ
                     IADDLSNLPLSYFSKHNLSDLSQTIMSDVEGIEHAMSHAIPKAGGMALFFPFISVMLL
                     VGNVKMGLAVILPTLFSFVLILLSKKSQTKANTKYYDTLRENSEEFQETIELQQEINS
                     FNLSKKVQDRLFKKMEESERIHLKVELSTFSVMALSSIFSYVSLAVVILVGVHLLLTG
                     EVTILYVVGYLLAAIKIKDSFDSMKEAVLEIFYLAPKIQRIRAMKETSIQEGSDSPLK
                     SFDIELRDVSFSYDNNTPILDHISFTAKQGEVTALVGASGSGKTSILKLVSRLYDYDE
                     GCILIDGYDIKRVSPASLFSKIAIVFQEVTLFNTSILENIRIGNSQASDEEVKKAARL
                     ANCEDFIEKLPDGYHTLVGENGSSLSGGERQRLSIARAFLKNAPILILDEITASLDAE
                     NEKRIQESLNRLIQDKTVLIISHRLKSIEKVNKIVVMDQGKLVDQGTHNELYRRSEIY
                     KNLIKKTKLSEKFVYEKEAQSR"
     gene            complement(18174..19928)
                     /locus_tag="U2K71_000398"
     CDS             complement(18174..19928)
                     /locus_tag="U2K71_000398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016398247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314515.1"
                     /translation="MKVYKKLFSYVPKMKYYGWLATLFSDFSVVLTVYGYYSIYKFLQ
                     SLIIVGDEYLAKSYAVQTVAWLTLGALFYIFSGLFSHILGFRLETNLRKKGISGLTEA
                     SFRFFDLNSSGYIRKTIDDNAAKTHMAIAHLIPDSAQAIVTPVLSVALGFFINFKIGL
                     VLIAMLVIGVFLLKKMTENTNFIQKYQESLDVLSSETVEYIRGIQVIKIFGAKVTSFK
                     ALNKAINDYAKFAYKYSLSGKTPFVLFQWLFFGIVCILIVPITFFITSLTTPKILAVD
                     LLMLFFLSGIIFVSFMRIMYVSMHLFEARYAVETLDSLYLEMKQDQLKYGNETQIKNC
                     DIAFENVSFSYKEECVLENFNLVLEQKRIYALVGKSGSGKSTIAKLISGFYNVDKGQI
                     KIGGKPLNHYSKDTIINTIAFVFQDTKLFNQSIYDNVSIANPQAPASKVFDALRLAGC
                     ESILEKFPEREHTLIGSKGVYLSGGEKQRIAIARAILKDAKIVIMDEASASIDPDNEY
                     ELQKAFQHLIKEKTVIMIAHRLSSIKSVDEILVLSEGKIVERGNHNELMAKHQVYKQL
                     VTNYENANDWRLDNEKFL"
     gene            complement(20038..20607)
                     /locus_tag="U2K71_000399"
     CDS             complement(20038..20607)
                     /locus_tag="U2K71_000399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MptD family putative ECF transporter S
                     component"
                     /protein_id="HEM7314516.1"
                     /translation="MKTKDYIFIGLATVISLVIYFFSIMVSSIGGAFGHSISPGIFGL
                     LSGIIFVYISYNYPRKGIFTIYTIVLLLFFTLMGGAYLPWFISSISMAILADIILSVF
                     GYDRAIPQVASWALMQLGSAAGQWIPIWFFTDRFRQDWIDKGQSAATMDAMIHYAVGI
                     WGIISVLVVASLSMIGVLIGRKVLKKYKK"
     gene            complement(20625..22040)
                     /locus_tag="U2K71_000400"
     CDS             complement(20625..22040)
                     /locus_tag="U2K71_000400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM7314517.1"
                     /translation="MFEFNNVSFSYKDVKEQALVDLNFSIKKGSLTLITGSSGSGKST
                     LLKLMNGLIPRLYDGKLVGEILFENNSLQSLTQESISKNIGYVSQDPRGQFFTTNTTS
                     ELVFSMENFGFTKEHMDKRINELAELLDLSNILDKNIFSLSSGERQKISIGCSLSLNS
                     KLIILDEPSSNLDFKATKKLADLLCDLKNKGFTIVIAEHRFYYLKELIDQVLFVKNRT
                     VKTTTIQLLKTENNNALRKFDIFNSSIEVPYIEKVSADVLKVEEITYENILKDINFDV
                     KVGDVIGLVGKNGVGKTTLLRILMKIIRPTKGKIIENKKNLSSPFLVMQEMDYQFFTE
                     SVRSELALGNEIVSKDQQEKILKKMELYKMKDQLPFDLSGGEKQRLLVSIASLSKTNL
                     FLFDEPTSGLDYINMERIADLIKDLKEKGAVVIATHDPEFLYKTCNRIIYLKNGGIKK
                     DIKLRVEDSTLIEQIFNDITK"
     gene            complement(22051..22719)
                     /locus_tag="U2K71_000401"
     CDS             complement(22051..22719)
                     /locus_tag="U2K71_000401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEM7314518.1"
                     /translation="MSKINPLTLITLNIFFPVIIFLGKGNIFQVGCFIIATVLVLMFR
                     KIKTCIYFILIYSVLFLVMKIIDAIKIEFFTIFFATSLYIVFRMLPVVMISWLLVSVY
                     TSNELLSSLEKIHLPKKIMLSITVVIRFFPTYRSEIRMIKESLKMRNIRLSFFQPLQY
                     LEFLLVPVLIRATMIAEEMTANAITKGIESPVKRSSYYKTNMSLLDYIILFSVTIFFL
                     SILI"
     gene            complement(22849..23433)
                     /locus_tag="U2K71_000402"
     CDS             complement(22849..23433)
                     /locus_tag="U2K71_000402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7314519.1"
                     /translation="MESGLSHFEKEGFQQTKIRQLCLTAGVTTGAFYKHFTSKEALFE
                     EIIQPHINALINMYNEEFTKYLLNLENIDIEKSWRENFNELDPFINYIFENKRIFDLI
                     LFQSAGTKYQDIVEKITSFVTKKTVCCLDKLKTIGMINQNLVFNEEEIHFYIYSFYAT
                     FYDILKHSYSKEKTMLLTRRLYQFSVPGWIELLS"
     gene            complement(23764..23997)
                     /locus_tag="U2K71_000403"
     CDS             complement(23764..23997)
                     /locus_tag="U2K71_000403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002382893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-dependent repressor"
                     /protein_id="HEM7314520.1"
                     /translation="MVQIKTTTILIYLLAIQELSKRKKKLKNNDLAKVLSVSPASVSE
                     MLAKLRYEDYLDIGFQLTDKGKKFIDDYKKGFK"
     gene            complement(24012..24962)
                     /locus_tag="U2K71_000404"
     CDS             complement(24012..24962)
                     /locus_tag="U2K71_000404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter substrate-binding protein"
                     /protein_id="HEM7314521.1"
                     /translation="MMRKWKAVLGSLGILIALFIFGACSINSKDKDKVASNEKLKVVV
                     TNSILADITENIAKDKIDLHSIVPIGKDPHEYEPLPEDVQKTSKADLIFYNGVNLETG
                     GNAWFTKLVKNANKEENKDYFAASDGIDVIYLEGQSEKGKEDPHAWLNLENGIIYAKN
                     IEKQLAEKDPDNKKFYKENLDKYIEKLDSLDKEAKSKFASIPNDKKMIVTSEGCFKYF
                     SKAYNVPSAYIWEINTEEEGTPDQIKHLVEKLRTTKVPSLFVESSVDDRPMKTVSKDT
                     NIPIYSTIFTDSIAEKGQDGDSYYAMMKWNLDKIAEGLSK"
     gene            complement(24959..25801)
                     /locus_tag="U2K71_000405"
     CDS             complement(24959..25801)
                     /locus_tag="U2K71_000405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM7314522.1"
                     /translation="MLQNFINGLYDFHFLQNALITSVVIGIVSGTVGCFIILRGMSLM
                     GDAISHAVLPGVAISYILGINFFIGAIVFGLLASIIITFIKSNSVIKGDTAIGITFSS
                     FLALGVILIGVANSSTDLFHILFGNILAVQDIDKWITIGVSVLVLVIVVLFFKELLIT
                     SFDPLMAKAIGMNVSFYHYLLMVLLTLVAVTAMQSVGTILIVAMLITPAATAYLYANS
                     LKTMIFISATVGASSSLLGLFIGYSFNIAAGSSIVLTAAFLFVISFLISPKQKFLRKK
                     ERSL"
     gene            complement(25803..26522)
                     /locus_tag="U2K71_000406"
     CDS             complement(25803..26522)
                     /locus_tag="U2K71_000406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEM7314523.1"
                     /translation="MLEVKKLNVCYNDFLALNEISLKIQEGALTGIIGPNGAGKSTLL
                     KAMLNIIPHQGEVSINNEDINKKLVKIAYVEQKADIDFTFPIKVKECVSMGTYAGMKV
                     FQRIKNAEWQRVSKALEKVDMGKYSNHQIGELSGGQFQRVLLARCLAQNADFIFLDEP
                     FVGIDLVSERIIMDTLKKLKHQGKTVLIVHHDLSKVKKYFDNIILLNRNLIAHGSVES
                     VFNEENLKKAYGDTIFIGEGE"
     gene            27214..27372
                     /locus_tag="U2K71_000407"
     CDS             27214..27372
                     /locus_tag="U2K71_000407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7314524.1"
                     /translation="MNKKQTCAFLGIDNNTLDKWIRIYNFPVIRVGGVVRFDVEDIKD
                     FMNTFKIT"
     gene            27444..28670
                     /locus_tag="U2K71_000408"
     CDS             27444..28670
                     /locus_tag="U2K71_000408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM7314525.1"
                     /translation="MPTKLSNGKYKTNLRYPKRFKEITGIASEKYQKTFPNRQLAIKA
                     ENDMKKKIEKVLREENANSLELKGKITFKKFYESKWLPRYELGQTIRSNRPPSDITIS
                     NTKDIFRLHILPMFGEYAMNYLNINTEIISDELTKKSKEYANIKIIKGYVRSMFDIAE
                     ILNYIEFNRTTKIIQSITAPKKNALEEKRIQEGKQALSSKELTNWIEAVNDDFNNHLL
                     TFHDYTLFMITLYLGDRKSETYALQWKYIDFEKQTVRLKHTLDKYQRKKFTKGRKDTV
                     IQVPEVVMTLLSEWKSVQADQLLKLKIKQTLDQYLFTYTKPSGEVNCPVHADYLNYRI
                     NSIKRRHPDLVHLSPHKLRHTYATIARQGGADMNQISNALTHSDISTTKIYVNTPDIV
                     DKAVFEAFQRGLNKCD"
     gene            complement(28758..29150)
                     /gene="rpsI"
                     /locus_tag="U2K71_000409"
     CDS             complement(28758..29150)
                     /gene="rpsI"
                     /locus_tag="U2K71_000409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002942223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEM7314526.1"
                     /translation="MAQVQYIGTGRRKNAVARVRLVPGTGKITVNKKDVEEYIPHADL
                     REVINQPFAVTETKGAYDVFVNVNGGGYAGQSGAIRHGIARALLEVDPDFRLALKRAG
                     LLTRDARMVERKKPGLKKARKASQFSKR"
     gene            complement(29166..29609)
                     /gene="rplM"
                     /locus_tag="U2K71_000410"
     CDS             complement(29166..29609)
                     /gene="rplM"
                     /locus_tag="U2K71_000410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HEM7314527.1"
                     /translation="MRTTYMAKPGEVERKWYVVDATDVPLGRLSTVVASVLRGKNKPT
                     FTPHLDTGDFVIVINADKVKLTGKKATDKIYYRHSNYPGGLKSITAGELRAKNSRRLI
                     ETSVKGMLPKNTLGRKQFTKLNVYGGAEHPHAAQQPEVLDITNLI"
     gene            complement(30128..30595)
                     /locus_tag="U2K71_000411"
     CDS             complement(30128..30595)
                     /locus_tag="U2K71_000411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HEM7314528.1"
                     /translation="MKFQQTKEVLNQLVADLSQMSVVVHQTHWYMRGPGFLTLHPMMD
                     EFMDDLNAQLDEVSERLITLDGSPYSTLREFADNTKIPDEIGRWDRTIPERLETLVAG
                     YRYLADLYQKGIEVSGEEGDDPTQDIFIEFKTATEKRIWMIQAHLGKAPEIDA"
     gene            complement(30715..32253)
                     /locus_tag="U2K71_000412"
     CDS             complement(30715..32253)
                     /locus_tag="U2K71_000412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HEM7314529.1"
                     /translation="MNVSIGLDIGTTQTKAVAFTDEAQEAASAYFRYPLIQETQGMAE
                     QDQELIFQGVCTVIKEVIKQLPSDTIIQFVSFSSAMHGLILLDEEKTPLSRMITWADN
                     RAADEAEKLKYTVLGKEFYRQTGTPIHPMTPLNKIKWLQNSQPELFKKTAYFVGIKDY
                     IFYRLFGELISDYGTASGTGYFDIHEFQWAEEVLDYLNIRIEQLPQVFPSTYQVTGLP
                     SKTAAELGLPNDISFVLGGADGPLSNLGLGAFGDTIAALTVGTSGALRFITTQPQFHP
                     EMETFCYVLDQKHWVVGGATSNGAGIFDWAGHTFLQEVVQSAKEKGENPYDALLSAIA
                     FVPAGANGLIFHPYLLGERAPLWDADAFGSFIGLQRQHTEKEMMKAILEGICLNLYRI
                     LKGVCQENQLTEIRATGGFAQSPIFRQMMADVLGYPLVFPTVTEASALGAVILGWRSI
                     GKIQSISEAQELIELKEQVGVQETDHKKYQKIYPLFVSTQKHLAQTYQQFAQLREELA
                     DSEK"
     gene            32333..33019
                     /locus_tag="U2K71_000413"
     CDS             32333..33019
                     /locus_tag="U2K71_000413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HEM7314530.1"
                     /translation="MLLYFIIGCCIGSFLCLTAQRIPLGHSIIYPRSHCVKCCRSLSW
                     YELIPILSIIVQRFRCRYCRCRLPFYYLLAEALCGGLFAWFFTFSSARNFSTFIWMLS
                     ALLFSLMDLFYFMVHSHTLFCSWAILWIFWLQAGVFQWQSVLLTFIVGGVCLRYGQSY
                     LGAGDTLLMLSWSGGLSLEELLQILFLASLFGISVFSVYFICHKKKLEKLPFIPFLSS
                     ALLIVLHLRY"
     gene            complement(33220..33747)
                     /gene="msrA"
                     /locus_tag="U2K71_000414"
     CDS             complement(33220..33747)
                     /gene="msrA"
                     /locus_tag="U2K71_000414"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HEM7314531.1"
                     /translation="MTKKAVFAGGCFWCMVKPFEEQPGIISVTSGYTGGHVPNPTYEQ
                     VTTGTTGHTEAVEIEYDPEVISYDQLVEIYWQQTDPTDALGQFADRGDSYRPVIYYND
                     EEERNIAERSKQALQDSGRFDKPIVTKIEPRSEFYPAEDYHQDYYKKNKLHYNLYREG
                     SGRAGFLRRHWGVKK"
     gene            complement(33831..34508)
                     /gene="pgmB"
                     /locus_tag="U2K71_000415"
     CDS             complement(33831..34508)
                     /gene="pgmB"
                     /locus_tag="U2K71_000415"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEM7314532.1"
                     /translation="MKIKAALFDLDGVLVDTARYHYEAWLVLANQLSIPFTEKENEQL
                     KGISRTESLERLLSFGKMEQKFSEKEKSAFAEQKNNLYLQAIQKMDETSVLPGAIAVL
                     EYLKKTNIKIGLGSASKNARLILEKTNLTSYFDVLIDGTQVSKAKPDPEVFLKGAQQL
                     NVPPNACLVIEDSEAGCQAALAGNMHVLGIGENINLPSAEYVIPDLTVFDQVRSFWHL
                     SEAVSRI"
     gene            complement(34505..35635)
                     /locus_tag="U2K71_000416"
     CDS             complement(34505..35635)
                     /locus_tag="U2K71_000416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEM7314533.1"
                     /translation="MRILIAPDSFKESLTALEAAQAIQSGFQEVFVQAEYDLLPIGDG
                     GEGTVSSLADSLRLKRQTAVVTNAFGKNKEIQYASDGQTAIFEMAEIVGLKDVPLNQQ
                     QPLRLSTRGVGELIRQLLQQGVRHIIIGVGGSSTNDGGIGMAEGLGYAFYDQDNQKVE
                     ALGENLGKIATVDKTNVPIELIKAKITVITDVTNLLCGKEGASFVFGPQKGLKENELA
                     VADQAMHDFYMRFAPQVIEASGSSAGGGMGAGLLAFASGKIVSGIDYVLDLLEFDKRV
                     QQADLVIVGEGRLDGQSLKGKAPVGIARRVPEGVPVIAICGSVGKGSEKAADVGISAV
                     FPILPAAVPLQEALQQATDNLHRTAKNIASLLQVVHFQGGNR"
     gene            complement(35635..37818)
                     /locus_tag="U2K71_000417"
     CDS             complement(35635..37818)
                     /locus_tag="U2K71_000417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEM7314534.1"
                     /translation="MKVEEKQFDLEYINKYASQMTIGNGFFGIRGSQEEDYRNQVRGM
                     FAAGVYNRPLGAESAELVNLPDVIRWEIKIDGEHFSLETMKVLAYERALDLEMGELKR
                     YALIETAQGKKVEVITCRVADQNNLHQAALKVVIKPLTEDCQVDVKTGIDGQQTNFGT
                     QQLVEKELRVFGDDLISASYQTTESKIDIGIAAKFNKAGVFQAKNRRITASFSEKIRK
                     NTSFTLEKIIVLSTSLQQTDPFKKSIAQAQKSVSYTEILEQSMSYWTDFWHQHRITIQ
                     GDSSFDQLAMDFAAYHLQIMTPRNDPSVSVGAKGLTGEGYKGHVFWDTEIFILPFFLY
                     QFPEVAKNLLLYRYDRLEGARQKARDYHYQGALFPWESAHTGQEETPKFAALNIKTGT
                     RQEVASAAAEHHISADLAYAVQEYVQATEDQSFLETKGKTLIHETAAFWLSRAVKVDD
                     HLEIHRVIGPDEYTEYIDNNAYTNYLAHYNVALAVKYQAGDPLFLKECQNFLDKLYLP
                     VPNKEQLIPQDDTYLSKPVIDLTTYKERPGSQSILLDYARSEVNELQVSKQADLVMLL
                     YLLPEYFSQPIVSKNLHYYENKTIHDSSLSKAIHAIEACRIQEMEWAYHLFQEACLID
                     LGPDPHSSDDGIHAASLGALWLAVIFGFSGITVTNGTLSISPRLPSAWEKISFPFIWK
                     NHRLLFVLTKKQIVIKKETKETLPIFVNGDQYQLEKELILERRGK"
     gene            complement(37815..38840)
                     /locus_tag="U2K71_000418"
     CDS             complement(37815..38840)
                     /locus_tag="U2K71_000418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM7314535.1"
                     /translation="MKKVAIIGAGQVAEKVHAAYYQKRSDVLELAAVVDPVSQRAEDF
                     CRRTGFSHAYTSVTQMLNEISPDIVSVCTPNRFHFEHVLAALEANASVMCEKPPAMTA
                     DQARKMAETADKNGQILAYGFQHRFSDEATMIKNHLDVLGDIYYVEANALRRSGVPGW
                     GTFIDKELQGGGPLIDYGIHMLDTAMYITGFPKVKSVRAYSFQKIGTKKNSGKFGKWD
                     PSKFTVEDAIFGTLELENGKIIQLNTSFALNIREDKLNVAFCGDQAGANLYPGELFED
                     VDGKLVSLQKTTPVNDPHELSMAHFVNRALGDQTQMIADGWQGYVIQNLVENLYRSAE
                     TGEVITL"
     gene            complement(38885..39712)
                     /locus_tag="U2K71_000419"
     CDS             complement(38885..39712)
                     /locus_tag="U2K71_000419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEM7314536.1"
                     /translation="MKFGTQDQAFFPRDILEKFRFIKDCGFEEYEIDGKLLIENIDTV
                     KQAIKETGIPVVTACNGYDGWIGDFIEERRLNGVRQIAEILRALSEVGGKGIVIPAAW
                     GMFTYRLPPMVSPRSKEGDYKAVSESLRYLDKVAEETGTVIYLEPLNRYQDHMINLLA
                     DARHYIDSNQLKHVQIIADFYHMNIEEDDISKALTTQKEYVGHIHLADNHRYQPGSGA
                     IDFTRHFQTLLQNEYEGYMVYECRLRGENEVKIYQESLQYLKNCLETARHLNDLEKE"
     gene            complement(39709..40776)
                     /locus_tag="U2K71_000420"
     CDS             complement(39709..40776)
                     /locus_tag="U2K71_000420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HEM7314537.1"
                     /translation="MKKLVALEPRIAGFKDYEDREIEKNEVRIRVIFATPKPGTEIVD
                     FRGLSPFIDEEFSEEWRLFLPREEGSQRGVVFGEFQLGNMIVGTIEETGSLVTEYQKG
                     ELVCTYGPIRETLITNGVDNYKLRKLDRLDQWKNAVCYDPAQFALGGIREAHVRAGDA
                     VAIFGLGAIGQIAIQLAKKAGATTIVGIDPIAQRRELAEKYGADYTLDPMKADVGFRL
                     KELTDKRGVDSVIETSGSAAALQASLRGIGYGGIISYVAFAKPFPAGFNLGREAHFNN
                     AKIVFARASSEPNPDYPRWNRKRIEDTCWELLMNGYLDCEEIIDPVVPFAESAEAYMR
                     YVDQHPEESIKMGVQVRGGEQ"
     gene            complement(40802..41638)
                     /locus_tag="U2K71_000421"
     CDS             complement(40802..41638)
                     /locus_tag="U2K71_000421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM7314538.1"
                     /translation="MKNKKSRKILFYATILLLLAVNLFPFLIMLMTSFKDSQEAIATT
                     QTIFPKEWTFEHYQDIFNAEIFPFIMYFKNSLVISLIASVTAVVIGISGAYALSKLRF
                     KARSKINASFYTVYMFSGILIVVPLFKIISGLGLYDTNTALIITMVVQTLPTAIFMLK
                     SYFDTIPTDIEEAAMIDGLSRIQIIIRIIIPLAISGIISVFVYCFMVSWNDYLFASIF
                     LSTSEKFTLPIGLNTLFSTPDYIWGRMMAASLVTALPVIIMYALSERFIKGDLVEGGV
                     KG"
     gene            complement(41635..42504)
                     /locus_tag="U2K71_000422"
     CDS             complement(41635..42504)
                     /locus_tag="U2K71_000422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7314539.1"
                     /translation="MNRPRKHSDLKLAMGLLAPSMLLLAVLVLIPMLSNIQISFFDMP
                     LNPNSDAVFIGLSNYLEILSDSEFYRSLGLTVAYTFLVVAGSTALGLAVAILLNRKFR
                     FRKTVRSLILLSYVTPSISLIFAWKYMFNNSYGIVNDLLVDRLQLFNEAPLWFDQPVA
                     SFFMVVLFAIWRYYPYAFISFLAILQTVDKTLYEAAEMDGANAWQKFKIVTLPAIKPV
                     LATVIVLRSIWMFYMFTDVYLLTNKVNILGVYLYQTAFAFNDLGKAAAISILLFIIIA
                     AVVLLSRKKVDLK"
     gene            complement(42574..43857)
                     /locus_tag="U2K71_000423"
     CDS             complement(42574..43857)
                     /locus_tag="U2K71_000423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEM7314540.1"
                     /translation="MKKIIVTTCLLATGFLLTACGESQSSNESQQTTIQFMHSSVEQE
                     RLSVINELVADFEKENPDIKIKQVPVEESAFNTKIVTLARSGKLPEVMEVSQDFAKVM
                     DKDELIDQTAVQNVIEEAGEDNYYDGAKNLVRSEDGKSYIAAPISGWVQGIWYDKEKL
                     SEAGFSEPENWDDILKIAQHFTDKENKQYGIAMPTAEGTMSEQAFSQFALSNGANVLD
                     DEGEVTIDTEKMREALSFYQNLSQYTMPGSNDVTEIKDAFMNGTAPMAIYSTYILPSV
                     YEAGNSENIGFAIPTQKDQAVYGTVSGLTISSGLDEKQKEAAEAFTAFLSQPKNMEKW
                     VLMSPGGAQPVNKQVVELDGYKENEVIKSFGELPSEIASAFDEVQVFGLVGEKNFTKM
                     GDITSSGVIGKAVNQVTVGNEDVDQALADAQKNTK"
     gene            complement(43871..45556)
                     /locus_tag="U2K71_000424"
     CDS             complement(43871..45556)
                     /locus_tag="U2K71_000424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphorylase"
                     /protein_id="HEM7314541.1"
                     /translation="MYTKLLQAIYRDSDLSEKTASMIEQKVSVYQQKKGQAGKLHWDE
                     EDIFLITYGDQFFEEQQPTLRTFKKFYQKYLADTFRVVHFLPFFPYTSDDGFSVVNYQ
                     AINDRLGTWEDIEEMRSETRLMFDFVCNHMSAESTWFQEYLNLNDTYKDFFIDLPLDT
                     DTSSVTRPRTSPLLTTFIDKNGKEKHVWTTFSEDQVDLNFKNPEVLLKMIDILLFYLE
                     KGASFIRLDAVGFLWKELGTSCIHLPETHKIIQLFRAIADEAAPGTVLITETNVPHKD
                     NISYFGNGENEAQMVYQFSLPPLTLQAIRSGNTSKLKEWLKTIDFASKKTTFFNFLAS
                     HDGIGLNPVRGILPEKTILELVEQLKKEGARVNYKKNPDGTTSPYEINATYLDALSSP
                     TDTTEWRSKRFLAAHSFLVTLPGVPAIYIQSVLGSRNDYKGVEKTGENRKINRQKFLL
                     ADIEKELQTDPLRTAVYGELTQMLSIRKKEKAFHPNSLIYLLDSADSFISFIREAKEE
                     KVLVIHNFSNQSAGYLLPKGDYLDLFSGQIIHCSQERKLIFQPYEYMWLKIIK"
     gene            45831..46856
                     /locus_tag="U2K71_000425"
     CDS             45831..46856
                     /locus_tag="U2K71_000425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7314542.1"
                     /translation="MKPTIYDIAKKANVSKSTVSRVLNNQPNISDKAKASVLQAIKEL
                     NYEPSKIARGLSGNGFDAILALSHRTSHTTYGNPFFSDVIQSISAVSEQNNFDLILQT
                     AKTPEEELEKCAKKIQEKMIRGIIILSSTMDETLLFELDQYNIPIVVIGKVGNDYTNI
                     YSVDTDNFQDSYDLVETLIKNGHRHIGCLHAPTEVYVSNDRLNGYRQCLFDHHLDIRN
                     EWIIDGGFRLEDSMAAMNDLMKLRERPTAIFSTDALKSLSIYKVAEQSGKQIPDDLSI
                     AGFSDPLLSSLFSPQLTRIDIPTNQLGIKATELLFQLIHHQYPEEKRIILSTEITLTD
                     SIQQQEM"
     gene            complement(47451..51125)
                     /gene="rpoC"
                     /locus_tag="U2K71_000426"
     CDS             complement(47451..51125)
                     /gene="rpoC"
                     /locus_tag="U2K71_000426"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HEM7314543.1"
                     /translation="MKWREPLLIDVNKFESMQIGLASPEKIRSWSYGEVKKPETINYR
                     TLKPEREGLFCERIFGPTKDWECACGKYKRIRYKGIVCDRCGVEVTRSKVRRERMGHI
                     ELAAPVSHIWYFKGIPSRMGLVLDMSPRALEEIIYFASYVVIEPGNTNLEKKQLLTER
                     EYREKREQYGQEFQAAMGAEAIKQLLDNVDLDAEAAELKEELKSAQGQKRTRAIRRLD
                     ILEAFRASGNQPSWMVMDVIPVIPPDLRPMVQLEGGRFATSDLNDLYRRVINRNNRLK
                     RLLDLNAPGIIVQNEKRMLQEAVDALIDNGRRGRPVTGPGNRPLKSLSHMLKGKQGRF
                     RQNLLGKRVDYSGRSVIVVGPFLKMYQCGLPKEMAIELFKPFVMRELVQRELASNIKN
                     AKRKIERQEDEVWDVLEDVIKEHPVLLNRAPTLHRLGIQAFEPVLVQGRAIRLHPLVC
                     EAYNADFDGDQMAVHVPLNEEAQAEARMLMLAAQNILNPKDGKPVVTPSQDMVLGNYY
                     LTMEEEGREGEGMVFRDQNEAVIAWRNGYVHLHSRIGVNPNSLGEKPFTEWQKERTMI
                     TTVGKIIFNEIMPPEFPYLNEPTDFNLTVQTPDKYFVEAGTDIPAHIKEQELVLPFKK
                     KNLGNIIAEVFKRFKVKETSKMLDRMKDLGYKHSTHAGITVGIADISVLNEKQEIIEN
                     AHKQVETITKQFRRGLITDDERYERVIAVWNAAKDSIQQKLMEGLEAKNPIFMMSDSG
                     ARGNISNFTQLAGMRGLMAAPNGRIMELPIISNFREGLSVLEMFISTHGARKGMTDTA
                     LKTADSGYLTRRLVDVAQDVIIREEDCGTDRGLEIQAIREGNEIIESLEDRLVGRYTR
                     KEVRHPETNELIIAGNQLISEDIAKQIVDAGVETVTIRSVFTCNTKHGVCKHCYGRNL
                     ATGSDVEVGEAVGTIAAQSIGEPGTQLTMRTFHTGGVAGDDITQGLPRVQEIFEARNP
                     KGQAVITEVTGDVIDISEDPATRTKEVTIKGKTDTRTYTVPYTARMKVAEGDMIHRGA
                     PLTEGSIDPKQLLQVRDVLSVENYLLREVQRVYRMQGVEIGDKHIEVMVRQMLRKVRV
                     MDPGDTDILPGTLLDIADFKDQNYNTLVSGGVPATSRPVLLGITKASLETNSFLSAAS
                     FQETTRVLTDAAIRGKKDPLLGLKENVIIGKIIPAGTGMQRYRDMEPKEVGVASENVY
                     SISDIEAQMAAEDAMNNQTK"
     gene            complement(51183..54800)
                     /gene="rpoB"
                     /locus_tag="U2K71_000427"
     CDS             complement(51183..54800)
                     /gene="rpoB"
                     /locus_tag="U2K71_000427"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEM7314544.1"
                     /translation="MAGHVVKYGKHRERRSFARISEVLELPNLIEIQTDSYQWFLDEG
                     LREMFEDILPIDDFNGNLSLEFVDYELKEPKYTVAEARAHDANYSAPLHVTLRLTNRE
                     TGEIKAQEVFFGDFPLMTEQGTFIINGAERVIVSQLVRSPGVYFHGKVDKNGKEGFGS
                     TVIPNRGAWLEMETDAKDISYVRIDRTRKIPLTVLVRALGFGSDDTIFEIFGDSETLR
                     NTVEKDLHKNASDSRTEEGLKDVYERLRPGEPKTADSSRNLLNARFFDPKRYDLANVG
                     RYKVNKKLDLKTRLLNLTLAETLVDPETGEIIVEKGTVLTHQVMETLAPFIDNGLNSV
                     TYYPSEDGVVTDPMTVQVIKVFSPRDPEREVNVIGNGYPEAAVKTVRPADIIASMSYF
                     LNLMEGIGNVDDIDHLGNRRIRSVGELLQNQFRIGLARMERVVRERMSIQDTETLTPQ
                     QLINIRPVVASIKEFFGSSQLSQFMDQTNPLGELTHKRRLFALGPGGLTRDRAGYEVR
                     DVHYSHYGRMCPIETPEGPNIGLINSLSSYAKVNKFGFIETPYRRVDRETGRVTDQID
                     YLTADIEDHYIVAQANSPLNEDGTFAQDVVMARAQSENLEVSIDKVDYMDVSPKQVVA
                     VATACIPFLENDDSNRALMGANMQRQAVPLINPQAPWVGTGMEYKSAHDSGAALLCKH
                     DGVVEYVDASEIRVRRDNGALDKYDVTKFRRSNSGTSYNQRPIVHLGEKVEKGDTLAD
                     GPSMEQGEMALGQNVLVGFMTWEGYNYEDAIIMSRRLVKDDVYTSIHIEEYESEARDT
                     KLGPEEITREIPNVGEDALKDLDEMGIIRIGAEVQDGDLLVGKVTPKGVTELSAEERL
                     LHAIFGEKAREVRDTSLRVPHGGGGIVHDVKIFTREAGDELSPGVNMLVRVYIVQKRK
                     IHEGDKMAGRHGNKGVVSRIMPEEDMPFLPDGTPIDIMLNPLGVPSRMNIGQVLELHL
                     GMAARQLGIHVATPVFDGASDEDVWETVREAGMASDAKTVLYDGRTGEPFDGRVSVGV
                     MYMIKLAHMVDDKLHARSIGPYSLVTQQPLGGKAQFGGQRFGEMEVWALEAYGAAYTL
                     QEILTYKSDDVVGRVKTYEAIVKGEPIPKPGVPESFRVLVKELQSLGLDMRVLDIEET
                     EIELRDMDDEDDDLITVDALTKFAEQQTAKELEKKAAEQVEDEKDDVIQNFETAEDNL
                     D"
     gene            55528..56496
                     /locus_tag="U2K71_000428"
     CDS             55528..56496
                     /locus_tag="U2K71_000428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM7314545.1"
                     /translation="MKKNIIIGLGAALLLALGGCAQKDNSAEASDGQKTIGVLQLVQH
                     NSLDAAYKGFKEGLAEEGYKEGDNLTIDYQNAQNNQDNLKSMSEKLVKEDPDLLLGIA
                     TPAAQSLLNETTTIPMTVTAVTDLEQAHLVTSNEKPGGNVTGTTDMVPIEKQIGLLLN
                     IVPDAKTIGIMYNNGEANSKIQADLAEKELKKAGVKVKVLTANSTNDVQQVTKSLAKD
                     VDGIYIPTDNTFASAASVVGEVAKETKTPVVAGAVDQVKDGGLATVGIDYESLGKQTG
                     IMAAKILDGKNPSELPVETANDLELYVNKDMAKALGINPDSIKEAE"
     gene            56514..57419
                     /locus_tag="U2K71_000429"
     CDS             56514..57419
                     /locus_tag="U2K71_000429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314546.1"
                     /translation="MTNLLSLFLSSASQGVLWALLAIGVYVTFRILDIADLTAEGSFP
                     LGAGIAAVSITNGYHPLTACLLGFLGSAAAGLVSGLLHTKLKIPALLAGIITMTGLYS
                     VTSRVMGAPNISLLGQPTIFHWVESFGITKDNAVLIVGLLFALIVVCLLVLFLRTETG
                     LAIRATGDNLAMSEANGINTDNMKIIGYMISNGCIALSGSLLAQNNGFADLNSGIGTI
                     VIGLASIIIAEVLLRNQPLFLRLLTVVLGAVIYRFILALVFELNVEPSDSKLASAFVL
                     VICLSFPQIQQKFHLPQKKKGGSVK"
     gene            57416..58222
                     /locus_tag="U2K71_000430"
     CDS             57416..58222
                     /locus_tag="U2K71_000430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7314547.1"
                     /translation="MNDVLTLENLHHTFEKGTINENHVLKGINLSLDPGDFVTIIGGN
                     GAGKSTLLNSIAGTFSVDQGQILLNGKNITKKSVVERSKSISRVFQDPKLGTAVRLTV
                     EENLALAMKRGKKRGFFRGVKPQDRTFFKDQLVRLNLGLENRLSSEIGLLSGGQRQAI
                     TLLMATLQRPELILLDEHTAALDPATSKTVMELTEKLITEQNLTAFMVTHDMEDAIRY
                     GSRLIMLHQGRIVVDIQQEEKQQLTVPQLLALFKQNSGTALKDDQVLLSS"
     gene            58358..59347
                     /locus_tag="U2K71_000431"
     CDS             58358..59347
                     /locus_tag="U2K71_000431"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HEM7314548.1"
                     /translation="MGKELDAMTTKDKVLQLLKKTPDFLSGEKLAQQLEVSRTSIWKA
                     IKELEKAGYRFEHGPTGYRYLPSDVLDAKEIQQELRSFIPDLKVQTMMTSESTMKDAK
                     LAAAQGDSVSKLFVADTQEQARGRFGRPFFAEPGRGIYMSLLLHPDKRFDELPQYTII
                     AASACVKAIESVTGRSASIKWVNDIYMDGKKVCGILSEAVSDMESGRISHIIIGIGLN
                     FSVPQNHFPADLQEKAISVFPDGQASVTRNQLISKIWQELFTYLSHPEKALDFYKEKS
                     FVLGKTVSFNQKGTTYVGLAKEIGDHGELIVQTNEQKMSLSSGEISLTSIRTQ"
     gene            complement(59664..60188)
                     /locus_tag="U2K71_000432"
     CDS             complement(59664..60188)
                     /locus_tag="U2K71_000432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF664 domain-containing protein"
                     /protein_id="HEM7314549.1"
                     /translation="MDYLDMLCESVDRAYDRFERLFEGVSIEEANRFPVEDRAPQIKS
                     MTWLAWHTARELDFQIADLAGTSPIWHSQDWESRFPMTIADNEQDWKHSLEQAKQIQA
                     NQIEDLLGYLRGAADFAISYLRRLEPSELDEVIDENWTPPVTRGMRLVSIIDDAAMHS
                     GQVIYARRLLGLRD"
     gene            60475..62115
                     /gene="scm"
                     /locus_tag="U2K71_000433"
     CDS             60475..62115
                     /gene="scm"
                     /locus_tag="U2K71_000433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Scm"
                     /protein_id="HEM7314550.1"
                     /translation="MKKRASLLVLVALLFNIFSYGVAVSAETYIPKDPGNIYNDLTGN
                     YGLLGIASQFHVFAKNKTTVNAHTDGNIATHELDAKNNFGTELHEGLLNQEINYIQKT
                     DSFANSSGIPTGSTRMNKFVVGSNVDVALQDGHPTVNGSRLDHLTPDDFYKDRAGHEY
                     IDFEQEFAKLNVAANDLATITPAKTYTAADFPDMNNRTIDLTDLSDTGFLLVNIDAEV
                     LTMNTPLQIINPNDQVVVFNVINSSSALNVQSPIKYNDRSNHETEDFSDANISWNFGN
                     EMTDLTISAPFQGTILAPNATIRITQNQDGTIIGTNVILDAATNRWDPNEIFISDNGT
                     DTTDTSDSTDTSTTSDSSDSSTSSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSTDT
                     SASSDSTDTTSTTSDSTDTSASSDSTDTTSTTSDSSDSSTTTSDSTDTSASSDSTDTS
                     STTSDSSDSSTSSDSTDTSSTTSDTRHASVSFSKTSSDNRTNFNLTNKTSNDNGNKSK
                     RALPKTGSQSNNWITLAGVILLVIGLRITFMSYKKSKR"
     gene            complement(62270..63256)
                     /locus_tag="U2K71_000434"
     CDS             complement(62270..63256)
                     /locus_tag="U2K71_000434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HEM7314551.1"
                     /translation="MKLHPMWENYPALSKELTETLKRMEETVRLKNKPVEQAIKETIH
                     AGGKLLRPAYQLLFSQFGPEQDRDKAIALAASIEMLHTATLIHDDIVDEADLRRGQPN
                     IRSQFGNVVSVYAGDYLFVCCFKLLSDYSTSLKSLQLNSRSMEKVLNGELGQMDDRYN
                     YELSVKEYLENISGKTAELFQLSCSVGAFESGTSERFAKKAGDIGLSIGMAFQIIDDI
                     LDYTKESQEIGKPVLEDMRQGVYSLPLIYSLQKNKPKLLPYLEKKAAMTEEDVDAVRK
                     IVEHTGGVEEARKLAASYTEKALKEIKKLPATSLRTKENLFSLTQLILDRKD"
     gene            complement(63279..63845)
                     /locus_tag="U2K71_000435"
     CDS             complement(63279..63845)
                     /locus_tag="U2K71_000435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gx transporter family protein"
                     /protein_id="HEM7314552.1"
                     /translation="MSKLQKMIYISLLVAQGVIIGLLENMIPYPFSFAPGAKLGLANL
                     ITIVAIFTMKKQDSFLLLWLRLFLTTLLGGTISTFLYSMSGALLSYFGMLLVKQLGPK
                     RVSIIGISAAGGFMHNVGQLLMASFIAQSWTVMLYLPVLSVLGILSGIAIGIAANYLL
                     RHVQTLQRFQKDYEQGTKQKWSPMFTKK"
     gene            complement(63859..64275)
                     /locus_tag="U2K71_000436"
     CDS             complement(63859..64275)
                     /locus_tag="U2K71_000436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NusG domain II-containing protein"
                     /protein_id="HEM7314553.1"
                     /translation="MSLKEFAEKSLLKPWDVFIILLLTVLSFLPVMIFGLQQNTATAE
                     KEAVLRVDGTEIKTFPLMAGEESNTYTYKDEHGDYNLIEIDGDRVRIKEADCDDQICV
                     RRGWATKNGETIVCLPHKLVIEVRSTDGGDEDSLIY"
     gene            complement(64732..66666)
                     /locus_tag="U2K71_000437"
     CDS             complement(64732..66666)
                     /locus_tag="U2K71_000437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM7314554.1"
                     /translation="MAKQNIVVVGAGYAGVSATKYLAKKLKKEDVAITLIDRHSYHTM
                     MTELHEVAGGRVEPEAIQYDLQHLFARQKNVQLVTDTVIGIDKERKIVKTKLGSYPFD
                     QLILGMGGEPNDFGTPGVKAHGFTLWSFEDSVRIREHIEKTVAKAALEPDDELRKAML
                     TFVVCGSGFTGIEMIGELIDWKNRLAKDYKLDPNEITLMVVEAMPTILNMLDRNDAAK
                     AERYLRKHNVELLLNAPIVEVAADHIKLKDGRNIPTHTLIWTAGVKGTSDAADFGLES
                     ARGQRLIANEYMQAKGYEDKNIYIIGDLVFYEEFPGTPTPQIVQAAEQTGHTAAANIV
                     ASIKGGEKHKFKGNYQGFMVSIGAKWGVANLFDKIHLSGFLAIVMKHIVNLKYFFDIR
                     SGYYMFQYLMHEFFHIKDDRNVTRGHSSRYGNVLWSVPLRIFYGLVWFVEASKKIIGN
                     GEVFKPSTWFGEGSWFTGKSVFPWTWEYNVTDVASGASAAGDATTAASGAGASAGEAA
                     TQAAHFGLSYAYGEEPMAVIEKSPDWFTKIMQVIMPNNDVAMFFQKFMVFFEIALALA
                     LIAGLFTWLCSATTIVLVITFCLSGMFYWVNIWFIFVAFALMNGSGRAIGLDRWVIPW
                     LQRTLGNWWYGRPSSQYGKK"
     gene            66947..67903
                     /locus_tag="U2K71_000438"
     CDS             66947..67903
                     /locus_tag="U2K71_000438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding protein"
                     /protein_id="HEM7314555.1"
                     /translation="MEWWGIKMKKKRFVTGFAVLAFSALVLGACGADDNGSSNSSSES
                     STAQSSTAKSTTESSAKVVAGGDLQDGTYKLEEKNYSNGYRAVFEMVVKDGKITESKY
                     DNVNENGESKTKDAEYNKNMEAKSGTNPEKYIPELNDQFLKAQSASGVEVVTGATHSS
                     ESFQNYAQQLIQAAQAGNTDTIEIDNGADLKDGTYKLEEKNYSNGYRVQFEMTVAGGK
                     VTESNFDYIDKDGKSKQDDTEYNENMKAKSGTEPKTYIPTLNDEFVKAMGEEDGSPAN
                     VEVVTGATHSSHSFIMYAQQLVNAAEKGDTQTIEVDNIVTEK"
     gene            67993..69096
                     /locus_tag="U2K71_000439"
     CDS             67993..69096
                     /locus_tag="U2K71_000439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEM7314556.1"
                     /translation="MRKKLLFSLITLGISFMVLTGCSNSANKTTTESSAATKILKDPY
                     SDEQFLLGTYVRIRVYDEGKESALKPAFDRVKELGDKITINQKGSEIDEVNEQAGIKP
                     VKVSDDVYTLVKRAYEYSQDSQGGFDMAIGAITQLWRIGFDDARKPSQEEIDQALKLV
                     DYHKIELNDKEKTVYLKEKGMIIDLGAIAKGYITDEVVKVLKKQGVTTAIVDLGGNVY
                     VLGHSPRGENQDWTVGIQDPNMARGSVLGSIKERNKTLVTSGIYERYLEVDGKKYHHL
                     FDPKTGYPFDNDIASVTIITDKSIDGDGLSTAVFSMGVKKGLEYVESELNNGTEAIFV
                     TKDDKVYVTDPIKDTFKLSEDSHYTMGDRSELK"
     gene            69109..70062
                     /gene="menA"
                     /locus_tag="U2K71_000440"
     CDS             69109..70062
                     /gene="menA"
                     /locus_tag="U2K71_000440"
                     /EC_number="2.5.1.74"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1,4-dihydroxy-2-naphthoate
                     polyprenyltransferase"
                     /protein_id="HEM7314557.1"
                     /translation="MTLKIFLEVVEIRTKVASVFPFAVGVLFSIAFFHEIHWLNTFLF
                     FIGMLVFDLATTAINNYMDYEKAHSEVYKYEENVIGRRNLSPVLIRNMILAMIAFVFV
                     IGVILTINTGWLLLLMGFICCFIGVFYTFGPIPLSRMPLGEIFSGVTMGLGIFAMVVY
                     VNTFGSDVYDLTVNFSSGQFALVGNLWAIAAIVLASLPLVFTIANIMLANNLRDLDRD
                     IENHRYTLVFYIGRPAGTVLFQLLMYACYFVILMGLLSGIYEWPILVTFLTLPIVKKH
                     LDQFKQTLPQPISFSYSIKNMVLFNASYVLGLLLTIIMQAI"
     gene            complement(70876..72222)
                     /gene="gor"
                     /locus_tag="U2K71_000441"
     CDS             complement(70876..72222)
                     /gene="gor"
                     /locus_tag="U2K71_000441"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HEM7314558.1"
                     /translation="MQIYDYIVIGGGSGGIASANRAGMHGAKVLLIEANELGGTCVNV
                     GCVPKKVMWQASTILETIERDASSYGIQADLKNVDFAGLVERREKYIDFLHGAYQRGL
                     DSNHVEAIKGYATFIDSQTIEVNGETYRAPKILIATGGRASKMTIPGGEYAIDSNGFF
                     ELTELPKRAIVLGAGYIAAELSGVFRGLGSEVMWAYRKERPLRTFDKMLSDNLIQMYQ
                     EAGIKTYAHHIAKEITKNNDEYTVLFENGETLTGDCVLFAGGRVPNTEKLGLENTNVE
                     LDKKGFIKVDKYQNTTDEHIFAVGDVIGKLDLTPVAIAAGRRLSERLFNGKKDSYLDY
                     KLVPTVVFTHPPIATIGLTEEEALEKYGENELKVYRSRFTPMYFALNDYRQKCEMKLI
                     CVGKEERIVGLHAIGVGVDEMLQGFAVAIKMGATKEDFDNTVAIHPTGAEEFVTMR"
     gene            complement(72356..73609)
                     /locus_tag="U2K71_000442"
     CDS             complement(72356..73609)
                     /locus_tag="U2K71_000442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEM7314559.1"
                     /translation="MMKEMTVKRKLKRPEILAPAGTLEKLKTAIHYGADAVYIGGNAY
                     GLRSRAGNFTYEEMAEGVAFAKEHDAKVYVAANMVTHEGNEDGAGEFFRSLRDVGISA
                     VIVSDPALIEICATEAPGLPIHLSTQASATNFETLEFWKEEGLERVVLAREVSMEEVA
                     EIRKNTDTEIEAFIHGAMCISYSGRCTLSNHMSMRDANRGGCSQSCRWKYGLFDMPFG
                     QERNSLTDTGEVEEEFSMSAVDMSMIEHIPELIENGVDSFKIEGRMKSIHYVSTVSNV
                     YKAAVDSYMEDPENYVCKQEWIDELWKVAQRELATGFYYNTPTENEQLFGERRKIPVY
                     KFVGEVLSYDEATQIATIRQRNLFSVGDEIEFYGPGFSHFSQTVEVMHNEEGESIDRA
                     PNPMMILTMPVAQPVKPGDMIRKRK"
     gene            complement(73615..74538)
                     /locus_tag="U2K71_000443"
     CDS             complement(73615..74538)
                     /locus_tag="U2K71_000443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="U32 family peptidase"
                     /protein_id="HEM7314560.1"
                     /translation="MIELITTVETIEQAEQLLAAGADTLYFGEEQFGLRLPASFTREE
                     QRQLVELAHKYGKKANIAVNGIIHPEKMKNVPEYLAFLKEINVDVITVGDTGIIYTMK
                     KNPELRIPYIYDAETLVTSARQINFWAKKGAVGAVLAREVPFEEMKAMEEKLDIPVET
                     LVYGATCIHQSKRPLLQNYYNYTKQDEQKDRERGLFISEPKKEETHYSIYEDSHGTHI
                     FASNDLNLSNELNELYQHHYCTWKLDGLFTPGEKFVNITRLFAEAKKQILEGSWDQEK
                     AEKMTSEISKLHPENRELDTGFFYIDPSAIK"
     gene            complement(74759..77251)
                     /locus_tag="U2K71_000444"
     CDS             complement(74759..77251)
                     /locus_tag="U2K71_000444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HEM7314561.1"
                     /translation="MEELFTERAQAVLEIAQEEAKRLKHQSVSSEHVLLALVVEQNGI
                     AGKVLREMDLNETEIYEEIEHLIGYGGIRSYPKGVFLPYSPRMKQVFALASSEVKKTG
                     SQKVGTEHILMSLLKDESIMATRIMINLGISLSKARQVLKQKMGLAGDSAGKGMNRRR
                     NVNVQDKRQTPQGTPTLDSLARDLTKLAKENKLDPVVGRSREVKRLIQILSRRTKNNP
                     VLVGEPGVGKTAIAEGLAQKIILGEVPEDMQEKRLMMLDMGSLVAGTKYRGEFEDRMK
                     KVIDEIYNDGQVILFIDELHTLIGAGGAEGAIDASNILKPALARGELQTIGATTLDEY
                     QKYIEKDSALERRFARIQVDEPTPEEAEVILQGLRTRYEEHHGVEITDEAVRAAVNLS
                     VRYITSRQLPDKAIDLIDESAAKVRLDQTDHLTKSTVIKLEIDELVQEKEAAIQKQDF
                     ENAAQLRRQEKALRKKLQKVSAIEAKQEQGYSDRVTEEDVATVVSEWTGVPLQQLEKK
                     ESERLLELEGLLHERVVGQDEAVKAVSRAIRRARSGLKDPDRPIGSFMFLGPTGVGKT
                     ELAKALSEVMFGSEDALIRVDMSEFMEKYSTSRLIGSPPGYVGYDEGGQLTEKIRQKP
                     YSVILLDEVEKAHPDVFNLLLQVLDDGHLTDSKGRKVDFRNTIMIMTSNIGATQIREE
                     KNVGFNVQDVTKDHKAMQKRILEELKKAFRPEFLNRIDETVVFHSLSKDEIHTIVQIM
                     SKAIIKRLKEQDIQVKITPSAIDVIGKAGFDPEYGARPIRRALQKEIEDRLSEALLGG
                     EIRLGDHVTVGASKGKITLNVRGPKKETVGSL"
     gene            complement(77258..77725)
                     /locus_tag="U2K71_000445"
     CDS             complement(77258..77725)
                     /locus_tag="U2K71_000445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CtsR family transcriptional regulator"
                     /protein_id="HEM7314562.1"
                     /translation="MAHQNTSDLIESYIKKILENSEMIEIRRIEMADLFNCVPSQINY
                     VINTRFTIQRGYLVESKRGGGGYIRIAKVRISDKKQLLEQINQLFDDTISEKNAFAII
                     QKLYEDQIITKKEGNLMLSAIAKNTLNFNEYEDHTRARILRAFLERLSYEDGK"
     gene            complement(77861..77934)
                     /locus_tag="U2K71_000446"
     tRNA            complement(77861..77934)
                     /locus_tag="U2K71_000446"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(77898..77900),aa:Arg,seq:acg)
     gene            complement(77992..78065)
                     /locus_tag="U2K71_000447"
     tRNA            complement(77992..78065)
                     /locus_tag="U2K71_000447"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(78029..78031),aa:Pro,seq:tgg)
BASE COUNT        23561 a        15823 c        13240 g        25460 t
ORIGIN      
        1 cctgcggtga aggttacttt gtggtagcct gcaggaatgt tttctcctgg attttcataa
       61 atatctttac ttacagttac tgctaaatcg gtcgtgccta cagaaccatc ttggtaaaca
      121 attttaatag gaattgttcc agtaatcggc tccgattctc ctacaccttt agctggagtg
      181 aatgtaacaa taccagtgtt tgcatcaaca cttgcattac cgaagtactt attatagaca
      241 tctgaaattg aaaaggcatc tttagctaaa ggaacatctg gtttatcaaa tgtaggtttt
      301 ccactttgaa ctgtcgtttc tggagttgtt gttataggta cgtatgttgc atcattttcc
      361 atagcttgag aagcttttac agcaaactct acatccacgt cgaaagttcg atttccgatc
      421 ttaacagtta caataccttt cgtactatca gtaacgttag aagtatcagg ctctgttttc
      481 catgaatact ctgcatcttc aggtaagttt ttattattcc gaattgattc tttagcatct
      541 ggtttaaatc caactaccac ggtttgttta tcaaccgctt ttggtgattc cttaataacg
      601 gttgaacctt cttctggttt atcaaaacct ggagaaacga tttggatttt taattcatct
      661 ttcgcgatta atttacttga atctacaccc gtaaattcaa attctacagt atctgaaatt
      721 ggagccccat ctttttcatt tggagcgata gttttttctg ctaatacagt accttcttta
      781 tcaacaagtt gggctttgtg acctgttccc gctaactcgt ggatgaatac tttcccctta
      841 actgttgtgt caacatcata tacttcttct aattcaggtt tcccgatata tggattcgtg
      901 tctccgctct cttctttatc gtcagggttt ccatcaccat caatatcttg caaataataa
      961 taacctgttc cataagtgtt ctggattaat tttttattcg tatccgacaa ccaagaatca
     1021 aagatgaaat cttcaccaaa tggtttattt tcttcaactt gtgctgttgc tttcatatct
     1081 tctaagattt catctaatgg cttagtcaat tgtaaaacat atcgaataac gactggacgt
     1141 ggacttgcat tagcaaaatc gataagttta gttaaatcat tattagttat tgaacctatg
     1201 ccataatttt catgaccttc ccgtcgctcg attgctaaat cagctacttt tgttccggat
     1261 tcaaacgaaa catcatatgt tactttgtca gcctgaacca tgtgcatctg tatacccgct
     1321 acatatggaa ctaatcgttc atcaattttg taattagcat accaaggcgt gccctttgcg
     1381 gaagatgaat atgagaaatt ataatttttc gttaacttat gatctaagat tatagctcca
     1441 ttagcacctg tggatcctga accatctttt gtttgagtat taggtttctc atattttgca
     1501 cttactgatc catgtttaaa ctggtcagga ttattttcag atacatctaa gctattatag
     1561 aaatcatctg aatctgttaa aaagtagccc gtactatcaa tacccggtac aattttatca
     1621 tcgtcttgat gacttaccaa gtagattcga tacattaaac gatcggtctt gagattgtct
     1681 atgcctatct ctttaaaaat ttcatctagg ctcttttcaa acgtaattac cccattagga
     1741 gcggtttgcg tatcggttgt ttctgcccca gcaaataatc catcacttgc acgaatataa
     1801 ttgaccttcc aaatatttgt aggtctaccc agcgaatcat taattcttac aaaggtccgt
     1861 ctagaagatc caattccttt ctttggatcg acctgaattt tttctacata tttagctaat
     1921 cgttcatcta attgaagacg gatttcccag tttttagttg atgctgcaat attatgtttt
     1981 ccgtaaactt caaatccaat tgtattgcct tttagtgtgt ctccagttag ttcttgagga
     2041 ctaaattgta aatccgaaaa gttataattt ttcgtataac caggattctc ctgaaaagta
     2101 ttccccgaag ctactttgtc acctacttgt acggagctta tcgctgtttc aagtgggctg
     2161 cttggagaaa tttcatcttg tgctttaact agttcagttg ttcccaagac aagaccaaca
     2221 ccaatcaaaa cagatgcaac cccaacactt aattttttga tactgtactt caatactcgt
     2281 ttctttgttt tttctagaaa tactctctta ttattcttgc taaccattat tcaaatcaac
     2341 tcccttaata attactttcc tagtaagaat aaaagataaa tctgatacgt attattcaca
     2401 gaataggaat cttactaacc ttatgttaac taatttactt agagaatttc atgaacatta
     2461 caacaaaaaa aagtacattt cttcatgttt ttttcggata tttgtgcttt ttaaccattc
     2521 agcagctgaa aacatataaa aagatatgta tgtattcttt ctgaaattaa gttcatttct
     2581 atgcgatgaa gcatgttttc aaaatattac cctttcttag attaaacttc agagtattca
     2641 aataacggaa taaggagcct tatcccgcat tactgaatga gactgtgaca taagtaaaaa
     2701 ggtgacttca aattccttct tcggaatttg aaatcacctt ctttataatt ataaagtatc
     2761 ggtttcacag catttctgaa tgattcctag aatcttaaac gaatgattgg agttatcaac
     2821 attttttcac tcgtttttag cgaaacaaaa aatgacattt gtcacagcct cattaaatta
     2881 ttgattcttt ttctttataa ttgaattgtc catatttaaa gtgtctagta ctctacatga
     2941 ataattaatt gctttcactc aaatacttat aatctattaa cttgtagttg tttaactttt
     3001 ctgaaaaagt tagacccacg taataatctt cccagctttc tctttcaatt aactgatcca
     3061 atttttgata atccataaat tcatcatgta tcaaaaagaa taaataactt gatattttag
     3121 catctagctt aatcagatta aataatgttt gtttaaaatt gctaccatta atatgactga
     3181 tagattctct agcttcttca cataaacttt ttactttatt aattactact ttgtcatatt
     3241 catttaacac actattatta ccattactta gacgctcaga ttctacgaaa caactcaaat
     3301 cagaaatttc ccaaaaaaat tttttgttaa aatccaattt cctcaactcc tccaaactac
     3361 gtaaatgata tagtctcata ataaaataaa acctctcagc taatgttgtt gaaattgtcc
     3421 actaaaccta attgatattg cttaggagat acacctgcgt actttttgaa tactctcgaa
     3481 aagtgtgaag aatctgaaaa tcctaaacct aaagaaactt cactgatagt atggtttgat
     3541 ttcaacatta ttttagccgt agaaattttt cgcttagaga tataattata aatcgtaata
     3601 tgcatttcat tcttaaagct ttctcttaat ttagtttcac ttacagaaaa ataattagct
     3661 atttccttta cagtaattct tttatagata tttaagccga tgaattgaac gacagaagaa
     3721 attaagggag aaagatgctc tatcttagtt tttccgattt ccttagtata ataaacaatc
     3781 ccagaatccc tcacttttaa gacctcaggt aacgatacag ctaattcgtt ttcttgtatc
     3841 aaagcatctc tactttgata cgcttctatt atatccattc ctaatgttat agacgtttgt
     3901 gctaactttt caaaaacaat gatagaataa tttttttcag accgaatcgt atctccacta
     3961 acacttggaa ttattccatt actcagatta aaaaccatgt tttctaaatt tcgaatatct
     4021 ccatttttaa catattccag tattttattt tcataaagaa acagatatat ttctggatcg
     4081 taattttgtg ataacagatt atctattttg atttgctcga tagttttact tagattttct
     4141 gtatattcaa ttaacggttc atggaataga tcttctattt ttccagtaaa aaaataatga
     4201 actaaaatca gaatatctcg aatgtctcct aaagaataca aagtcaaatt ttgcaatgaa
     4261 ttatagagtc gttctctatc agccattgca acattcaaat tgttcatttt tagtttaaaa
     4321 aaaactttat caatattatt tgctctaaat ggaccaaata agtaatatct ggttttaaac
     4381 atgtatagaa gaaacatctc attaaaatca ccagtaaaga aatggaaatc tttagttttt
     4441 ttaatattat cagttagtaa ctttttataa tcataaaaaa aataagtagt tctatctgag
     4501 cgatactctt ttataatact gaattcggaa ttaaaaacca cgataggtaa ctttaaacaa
     4561 acatgaagag actttaataa gacttcttta tctgaaaaca cttttgtttc tctctttcct
     4621 ttctacatca ttaaatagta ttttatacgt tatctaaaat actattcctc tgtcaaaata
     4681 agctaactta cttcattata aaatatccac ttatcgcttt catgtatatt ccaccgtaaa
     4741 aagtacattt tatcaccgag aactcatcat ttttcatcat gatgaatact atttacatat
     4801 atctagttca aagacaataa ttattatgat ccgttattat ctaagcacta tacagataat
     4861 aggaattttt cgtttctaaa tattccaatt tgggaatcca ataaaaattt tcgttcaaat
     4921 acgtgtctat tcgaactctt ttctagttgt taattatgaa gggggaagaa ataaaaaata
     4981 caatctatac acgaataaga atattatcca aaaggaggtt ttagaaaaga tttattatac
     5041 gtaaaaagct caagacattt tcttagccat aaggacatgt ttgatattta agaagatata
     5101 tgaattttct tttctaagta taaagataac tatccattat caactaaaaa aacatttttg
     5161 aaaataaaca tcagcagtcc tatcaatgca tgtgagctta tagaagttat tcgcagcgca
     5221 gcataattgc attagcttct tttcgtgttt tttgcctctt ttccctttaa atcttaatgg
     5281 aggaggtgtg caatatgtca gataacgacg aacgcgtaaa tgcccagttg attcgattct
     5341 ttgaaaaggt cattttgaac accgctaata cttattataa aaaacaagaa gaaattcaaa
     5401 aacatgaaca acttgatcaa ctcccaccat atttgtttac taaagatact ataaaattta
     5461 aacaaccgat taatgtttta aatgtgactc tccttgttga agatataaaa ttagcaaaaa
     5521 tccttccttt gttgaaagaa cctgaacaaa tttttcttat cgaaaaattt atctttgata
     5581 aaactgataa agaaattgga aaggttctgg ggattacaag acagggtgcg acaaatttaa
     5641 aacatcgact ttacaaaaaa attcaaaaaa tgctggttta atatctgcat aataactaat
     5701 tttgtatgct tctttgctcg ttctcctcct tcccaaagaa atagattata agtccatggg
     5761 aagaaggtag atttatgaat tctttaaaag aagatttcat tttagcaaag gctggaaatg
     5821 aagaagctgt tgaggcaatt ctcaaaagat ttagctcttt gattcataag caatcctgag
     5881 aactggaaaa tatgatcaag attgttatca agaatgtatg cttgcaatat atctagctat
     5941 ctcgaaattc gaaattaaag aataactcaa ctaagggagt ctgtgacata agtaaaaaga
     6001 tagtttcaaa tttcgaacaa taaatttgaa actatcttct ttataattat aaagtatcga
     6061 ttttacagcg ttctataatg attcatagaa tctaaaacga atgattttaa ttatcaacgt
     6121 tttttaattc gtttttagct taaaaataac ttttgtcaca gtctcactcc taaaattact
     6181 ttatttacgt atgaagtggg caggaggtcg aatccagaac tgggctatgg tacttcaacg
     6241 aggacttctc ctctagaatt gaaaagcatg cgatttacct tccgtaacga cggtagattt
     6301 attcaactat gtgtccaatc aaaatttgtc tgatttcaaa aatagtcaag agaattttgg
     6361 ttgttaaaaa ctcttatcta tcaacttcca cagtttagtt gacaaaccct gatactaagt
     6421 tgggctatga tacacttttc ttgactatca gttacgttac cggaaggaat gaactctatt
     6481 gagaaaaaca tatgatcgta aatttaaact aaagatttca caggatatcc ttgagaaaaa
     6541 agtaactacc aagaaaattg ctgaagaata caatatctct cgtccaacaa tttcccgatg
     6601 ggtttcagaa tatcgtcgat acgggaaaaa tgcttttgct ggacaaggaa aaagattacc
     6661 tgataaagca gatttctaca ttcttgaaca ggaaaataaa cgactaaaag aggaagacga
     6721 gtgttctacg aacacaaaga tcgatacgga tctccaggga tcactcaaaa actttacgat
     6781 gaaggaatcg aaatcaataa acgtgttgta gctgttctta tgcgtgaaat gaatttatgc
     6841 acaagaggat ttcatcgacg caaatcttct catggtagag aaaaaacaat caaagaaatg
     6901 gtaaaaaaat gttgaatcga taatttgaac agaaccaaat tgatgctgtt tgggtgatgg
     6961 atattactta cattccatgt aatgacggtc gcctctattt gtccacctat attgacttag
     7021 caactcgtat tctacgttac tatatggtag attcacacat gaaaaaagag attgtcgtcc
     7081 gtccattaca aatttattaa ggagaattgc ctacggtaat tcattccgat agaggtagcc
     7141 agcatcggtc ttttatttat caagagctgt tgaacgacca ccacatcaca cattcagtga
     7201 gccaaactgg gactcctgtt gacaatgcag taattgaagc gtttcatcga tcaatcaaat
     7261 gagaattaat cgactccaac aaatataaaa gtaaaataga gatgaaggtg ttaatccaag
     7321 attatttaga agactactac ccaaacaaaa gaatccatac aaaatttatg atgactcctt
     7381 gacaatttga agcgaagaaa ataagggatc tataatatct agtgttggtg acgccttctg
     7441 ttgccaatac tttttctttt ttctaataaa cgcaaacaga caccttaaaa tcctttagaa
     7501 taaagtcgac gaaaaccatt caaaggagtg tttcaagatg tcctattcag agtttaccaa
     7561 agagttacta gatattctag acctaaatct tatctttcat gaagatgcct ttcgcaaaga
     7621 gcgaatcaat gacgaaacat gttttgtttt tgatggcaca ttgacctatc aaccggagga
     7681 atgctttcat cgccactatc aaaatgaaca aacgatcatt aaatagggct ggaaaaaagt
     7741 atccatctta ttgaatgatg tcagtaacta caagaccatt ctgcggatta acaaacagcg
     7801 cttcaaatgt aaacactgtg gaaagacttt tttagcagaa gattcagtta gtgatcgtcg
     7861 ctgctccatt gctcgcagag tcaaacaagc cattcttgag ctattaagcg aacctctttc
     7921 tatgtctttg atcgcacgta tgaagcacat ttctccgacc actgtcattc gaatacttcg
     7981 cagccttcgg cctaaaacca tttctttgag ccagctgtta ccagaagtgg tgtgtttcga
     8041 tgaattcaaa tcagtgaaaa atgtctcggg agccatgagc tttgtcatga tggacggcaa
     8101 gacccatcaa ctgattgata ttgtggaaaa tcggcaactc aatcctttac gcgactattt
     8161 cttgcgctac cctataaaag tccgtgaaca agtgcgtcta gtggtttcag atttctatac
     8221 accctatcgc acattggtga aagagtgttt tccgaatgcc cgaatcgtcg ccgatcgctt
     8281 tcatattagc caacacattg gtcgggcttt taccaaccac cgaattcaag taatgaagac
     8341 ctttaaaaaa ggggatcgtc ggtcgaagca cttaaagaaa tactggaaac tacttcaaaa
     8401 gaatgcgtgg gaattgaaag gtcagcatcg ctactggcgt ccgtcatttc gagatcatct
     8461 gacagaagca gagatagtcg atcggctatt atcttatgat gattccttaa agcgaggcta
     8521 cgaagtctac caggattttc tttccgttat cagaagacaa gatgttcctg aattcgtcgc
     8581 gctcctaaaa gaagactaca aggagttgcc agagcactac caaccagtgt tcacgacctt
     8641 caagaaatac caaacagaaa tcaaacgagc gttgcgcgtt ccctattcca acggccctat
     8701 agaatgtttg aataaccata tcaaagtact aaaacgaatt gcctatggct atagaaactt
     8761 tcaaaactac cgagaacgaa tctttttata tcgaggaaaa tattttaaga aaactaagaa
     8821 tactacccaa ctgactaaag ccagaacaac aacacggcta gacaagatag ctgtctagcc
     8881 gtgtgagtat ctcgattatt ctattgaatt tcttcaccaa cactcattga cgaagagcca
     8941 aaataagtaa tctaaccgac tgttaactaa accattgcag gtcccgttaa agatacttgt
     9001 gcctgttgtc accgcttctt tttcttgtcc acaagcgctt aacacactta ataaagcccc
     9061 tgctattatt aataataata tctttttctt attaaaaacc tcctcatcta tttccagatt
     9121 agtaaatgta gtaatctgtg ctttcttttt tctcaatact aataactaac ttatggggta
     9181 aacaaatact cgtttgaccg ggttttgaga tccatccagt tctaaccgcg atttgatcag
     9241 gactattatc ttctttaact cgaattcttc catctttaat ctcaacaata ttatattgtc
     9301 ccttcgcagg atgataattc acgagtttat gattgatctt atccaaatcg aagcgatcca
     9361 tttccttgcc atcaattcga acgatggcat atttgatagc atcttcagtt gtttcattct
     9421 ccttttctat agagaataca aataagggaa taaaagaggt aataatcaaa atgccaataa
     9481 ttatgacatc gcctatcttg acaccagctt ttcttaaaaa tttcattaac gatatctggc
     9541 taaacatatt agactttttc catggaaatg gtgaaatttt tcggaaatta tctgcattaa
     9601 gagaatcagt taaacaaccc tcaagttgtt ccttaataca ttgttgatcc atcttcatac
     9661 caataaaaac tagtttatta atgcgttcac caaacaacgg atgccaggct tccttcaatt
     9721 cgggattttt ttgtataatc atttgacact caattttagg taaactagct acccaataat
     9781 ttaatgattc aatactttta tagctacccg atgtttcaaa gatcgtcgaa aaatcactat
     9841 gttgggcaaa ccaaagaaac ccttttgctc ttgtaatatt gccaggaaaa tttttatccg
     9901 taagatgggc aaatttttgt gcgtcaagga gtaaccgatt tcgatagata aaacttgaga
     9961 taccatattc ttctgtttca ggaatatgat tttgatagcc aagctctaac tctttgaacc
    10021 agcccactga ataggaagtc tcatctatat taaaaagatt tgtatttaga attttcgata
    10081 agtctacttc acctttaatt gtcctaatta ttttggcatt ggactgtaac ttagcaatta
    10141 gaccgttaat tttgtaaatc gtcttggttg acactaagtc actattattt aaaagtaata
    10201 tgttacaaca ctcgaattga tcaattaaca agccacgaat atcctgttct tcttcaacta
    10261 cattaccttc agcttttctt tcctgaatct tttcacccgt ttcgaaatta tcccaaaaac
    10321 gttttacatc cactactata ataatggcac caatctctaa tcgttgggtc aaatcaatgt
    10381 ctaaagggtt tttagctaat ataattgctt gaataattgg aattggtttg ctgattcctg
    10441 aagcttcaat aacaatttga ttcaagtcag aaaattgcac taattgatct agttcaacaa
    10501 tcaaatcttc gcgtaatgta caacaaatgc atccattcgg catcgaaaca agtttttctc
    10561 cagttctttt aaaacctttc ttgtcgacta ataccccatc aacatttact tcccctatgt
    10621 catttacaat gacagcaatt tcctttccgt gatcaccgta cagaatatga tttagaagta
    10681 tcgtttttcc tgaaccaaga tagccagtta agattgtaat aggtattttt ttcataggga
    10741 acctctttct ataccttgaa tttacacttt aagtgcaaca cataaaaatg agtcataatc
    10801 gatatccaac tagtataagt ttgtcgagaa cactcctaga aggaaaataa ttatgagcca
    10861 gtatcgtcat cttactatac tcgaacgaga aagaatcttt ctgcttcacg aagaaggatt
    10921 ttctattagt ataattggca acgacattca gcatagcccg tttactattt tacatgaact
    10981 gagaagaaac aagaaaaaaa ctattcacct tccaaagcgg aaaaaaatta tgcttatcga
    11041 agaaaaaaat gcggacgtta tcacattcta aatgatccgt gagtttggtc aattgttcgt
    11101 catttatttg tcgattttca gtggtcgcca gaggaaatct cgaatcgttt gaagcatgaa
    11161 catatggact tttcaattag ttttatcacg atctaacgtg caatttgcaa tgatcttttc
    11221 gatacagaac ccctctcaca cggcaataaa ggcctgatcc gttcattgca ccatcgagga
    11281 aaaacaagac atacaaaaaa tcatgtcgag tctcgtggaa agatttgcct tactcattcg
    11341 atccatggcc gtccaaaatc cgcagatgat agaagtgatt ttggtcattg gaaagccgat
    11401 attgtagctg gcaaaactgg tggtacttgt ttagtgactt tgacagacag aaaatcatgc
    11461 ttccttttag caggaaagaa ggtccccaga aagaaagccg cttatgtgag cgagaaaatg
    11521 attgaacttt taagcagtat tccaaatgag gcggtcaaaa cgatcactcc agatagagga
    11581 aaagaattct ccaatcattt cgcagttaca gaagcattga ataatatgcc cttttatttt
    11641 ccagatcctc atgcccttgg caacaatgaa caaattaaaa tacgaataga ctcattcgag
    11701 aatacttgcc aaaatcgaaa gagatagcta cggtagataa ctcaacgata aatcagatga
    11761 ttgaaaaact gaataacaga cctagaaagt gtttagattg gagaacacct tatgaagtct
    11821 tctttgataa agtgttgcgc ttgatttgac aattcaagac ataataaaaa gcagatcaaa
    11881 tccaaataag ggaaaaatga tctactctgg tattatcaat taattggcca agctattatg
    11941 ccgttttgct aaacgtctac ttcttccagt attgaccttg ataaaacaac aatcaggatc
    12001 ccgttttaat actctatgaa ctgtgtcaat actaacatgt aaagttgcag caatatcagc
    12061 taatgtactc ccatttaatc tcatttgctt tgcaaatcga taacgccgat aagcatcaat
    12121 attcttggaa tccatagaca ataatacttt tcttaccatg tatttcctcc aaaaattgaa
    12181 ttttaatgag ccatcatttc ttgagcaatt tcatgtttag acaatgaagc tggatagaaa
    12241 gtaggccaat tatgcaattc attatagagt ttttcttgac tttctccacc aaagaaaata
    12301 tggaaatgct ctgccttact tggtgcaatt ccatgatcac taaattgaat atatttaaaa
    12361 cgaccagcac ctgcatcatc agtttcaaaa ttaaaacgaa caccacggtt tcctttttca
    12421 taattcaaaa ttttgtatcc aacatatttg tatgtgtatt gatgatgttc accgttgact
    12481 acaaaatcaa tggtgttgtc tttaatgttg atagtatcaa tatctgtctt atagccagta
    12541 gtataataat ctttgtactc ggcagctgtc atgtctttat tgatttttgc tttgtaatcg
    12601 aaaacttcat ccaaaattcc atcttttaac aacggataaa cagattgcca ttcgcctgta
    12661 taatcactca attcgcgatc cttcacatcg gcatctttaa agtacccatt agcaactgtc
    12721 ttttcatcct tcgcttcttt ttctggaact acctcttcac cagcaaccat agttgttttt
    12781 tctaacgctt ttagattgtc ttccattact gaaacataat cttcaccatt atccatttgt
    12841 tcttttgtaa gactttcaag aggattcagt acttccaatt ttatcccagc ttcgttggcc
    12901 aaagtttttg caattttatc attagcattc ttttcaaagt aaatataatt gatcttattt
    12961 tttttaacat actcttttaa ttcagctaat cgaccagaag atggctcttc ttcaggactt
    13021 agtccagcaa taggaacttg tttcaatcca taatccaaag ctaaatatcc aaaggctgcg
    13081 tgttgcgtca caaaactttt ttgtttcgct tctttaagac tagtggtata gtcagcatcc
    13141 aaccgtttca gtttagtcaa atacttttca gcatttgtct caaatacttt tgcttttttc
    13201 ggatacaatt taactaactg atccttgata tttgaaactt ccttaattgc cattttgggt
    13261 gaaacccacg tatgaggatc tagttcatga tggtgatctt cttctccgtg atcatgatct
    13321 tcttcttcac tacctggcaa taaaatcatg tcttttgttc cttctactac attaggccca
    13381 gctttcttcc aactttcttt agcttttggt acccatgatt ccatgttttc gttatgatat
    13441 acaaaaacat cagcatcatg agcttttgcc atatccttag ctgagggctc atagtcatga
    13501 ggttctgatc ccgctggaac cattaaatcc acgttcgctt catcgccgac gatgtttttg
    13561 gtgaaatcat acattggata gaatgttgtt acaatttcta attttccatc tttatcattc
    13621 gatacttttg catcattagt gctggagcaa gctcccaata atcctagagc tgctatccca
    13681 ataagtccta cactgatttt cttcattctt tttccttcct taattatgct aatttcttca
    13741 ttttatttcg tttatgttct ttgataattt ttaatgccct ttttctagtt ttaatattcg
    13801 ggcttttaag tcgtcgatat gcactagata tatccgtttt ctccattaga aattaccccc
    13861 aatcactact tatcattatt tgctttccgc cagtaattta tcaaaactct gtttgatcct
    13921 ttcttcatcc aacatttttc cgattagaat aatagtggtt tggtcttttt gtcgagtggt
    13981 gtattgaaaa ttaacctgtt cgtttacctc ttgcatggca actataaaat cctgctcttg
    14041 taaagcaatc aatcctttaa aacgatacag gctcccttgg ttggttaaaa tcagccagtc
    14101 aatccattgg agtaaaaaat ctttttttat gtcttgatct gaagttaaga gaattgattt
    14161 aaactcatgt acttgatggt gttgatgata gtatttgcca ttatagtgat tttctgatga
    14221 atcactttca aaaactaagg gatgattaaa ttttttcaaa ttataaaaat cctttttttg
    14281 aatcgtttca ctatgtgtga acattcgaat atcggataaa gggttaatac ttttgatttc
    14341 tctctgtaat ttttttagtg cagataaatt caagttttgt tttctagaca caaaaatgcg
    14401 atctgccatg acaagttgct taagggcttg tggttctctt tcaataagtt caaaactgtc
    14461 actatcaata acagtcaata aactatcgat atatactctt cctcttagtt gaggcgctgt
    14521 taaaagtgtt tgaatgattg gttggggatc tgctatacct gtagtttcaa tgatgatttg
    14581 tgaaattggt tgttgatgtt cttctattgt ttccagtaaa gaaatcaata ttgctaataa
    14641 atcactgcga agactgcaac acatacatcc attatttagt tgtaacaccc gttcctcgct
    14701 atgaactaat aatttgtgat caatcccaac ttcaccaaac tcattttcaa ttataataat
    14761 ttcttccctg gggtagggtg agacttgcaa ggcttgattg attagcgttg tttttccagc
    14821 ccctaaaaac ccagaaacga ttgtaactgg tatgcccatt ttgtcacctt tttaccactt
    14881 agctatttca cttctttaaa aattgcccgt ctgcgcaatt ttggcgaata cttttttaat
    14941 tctaaccgtt cagggttatt ccgtttattt ttgctggtga gatacagacg ttctccagtt
    15001 tcgacacatt ccaaaataat attttgacgc ataaatcttc ctccttcaat acttatcgat
    15061 cttcaagata tgcttgaatt tgttgtgcat cttcaaaagt taactcgcca gattccaatt
    15121 gtgaaactaa attttctttc atatcaacag gtatctccca ataaccatcg atacattgaa
    15181 ataactcctc gtcatactct tcaaaaattg gatagattct taaaaaatca tcaaatgata
    15241 agccttggtc ctcatagtaa tctttgacta atccattttc aattgcctgt tgggtcggta
    15301 aacccgtttc agtaataatt cctctctcta ccatcgaacg ataaaaagtg catttatcta
    15361 gcgattgctg ggtcgtttca agataatact ttaatccttc ttcaactaaa ggtgggtaaa
    15421 taatttccat tttgattccc tcctaaaagt tatatattaa actattttga agctccttta
    15481 attactttct ttccattata gtaccctttt aaagatacat gatggctacg tctataatca
    15541 ccattcgctt cgtcaaatga aatttctggt tttactacag tttgatgagt tcttcttaac
    15601 cgtttcttcg ccttagatgt ttttcttgct ggtactgcca tgatatttcc tccttttacc
    15661 aacttgcttt ttttactcca ggaatcaacc cttgatgggc aagttctcta aacttaatac
    15721 gggacatacc gaactttcgc atataccctc ttggacgacc atccgtctga tcacgaagcc
    15781 tcagacgatt cggatttgaa tctcttggca atttagcgag agcggtgcga tctcctgcgg
    15841 cttttaattc ctgacgttta tctgcatact tttcaatcat cgccatttgt ttctttgctt
    15901 tagcaatttt cgactttttt gccatatacc tttatactcc ttttattttg atttagacac
    15961 ctcaaatcgt aatcgttacg ttttgacact gatactttac caacaagagt tgctcttgtc
    16021 aacaataaat aacatgaaat aaaactcata tgaatagata actatttgaa aacgaattta
    16081 tcggtacata gagataatca aatcatttaa aagaatatgc agtttaggct ctgaaaaata
    16141 agaattcact tttattagtc caatcaaaca caaataccgt tttaagtagc ttttcctcta
    16201 cttaaaacgg caatatgaaa gaaactataa ctttttattc aatcttttat gtttaactgg
    16261 tttatcgcca attttttaaa actttgatta ttccttatat tataagaata gacattaagt
    16321 gaactaattc aatcatactg attctttcgt gtattcaaaa ttagatgctg tttaataaaa
    16381 ttattctttt tgaaaaagaa ttataactat atcccaaaaa ttgtaggtta atttaaagcc
    16441 tatttcatca tcgtgattga gcttcttttt catagacgaa tttctcagat agtttagttt
    16501 tctttatgag atttttgtat atttctgacc ttcgatacaa ctcattatga gtaccttgat
    16561 caactagttt tccttgatcc atcactacaa ttttatttac cttttcaata gacttcaagc
    16621 gatgcgatat aattagtact gtcttatctt gtattaaacg atttaaactt tcctgaatac
    16681 gtttttcatt ttccgcatct aaactggcag taatttcatc taaaattaaa attggcgcat
    16741 ttttcaaaaa agcccgtgct atggataggc gttgtcgctc tcctccagaa agtgaacttc
    16801 cgttttctcc gaccaatgta tgataaccgt caggtagttt ttcaataaag tcttcgcagt
    16861 tagctaaacg agctgctttt ttcacttcct catctgacgc ttgactattt ccaatacgta
    16921 tattttccaa aatacttgta ttaaacaaag ttacctcttg aaaaacaata gcgattttgc
    16981 taaacaaaga agctggagat acacgtttaa tatcataccc atcaattaaa atacagcctt
    17041 catcataatc atacagtcta gacactaatt tcaagatact agttttcccg gaaccactag
    17101 cacctactaa cgcagtcact tctccttgtt tagccgtaaa agagatatga tctaaaattg
    17161 gggtattgtt atcatatgag aaagagacat ctcgcaactc aatgtcaaaa gatttcagag
    17221 gactatctga tccctcttgg atactggttt ccttcatagc tctaattcgc tgtattttag
    17281 gagctaaata aaatatttca agcactgctt ctttcataga atcgaaggaa tcctttattt
    17341 ttattgcggc tagtaagtaa ccaacgacgt agagtatagt cacttcaccc gttaacagta
    17401 agtgaacacc tactagaatg actactgcta aactaacata tgagaaaata gaggataagg
    17461 ccatgactga aaaagtactt aattctacct ttaaatgaat cctttcactc tcttccattt
    17521 ttttgaaaag tctgtcttga acttttttag atagattaaa gctattaatc tcttgctgca
    17581 attctatagt ttcttgaaat tcttccgagt tttctctcaa agtatcgtaa tatttagtat
    17641 tggctttcgt ttgggatttc tttgataaca agattaagac aaaactaaat aacgttggca
    17701 aaataacagc taatcccatt ttgacattac caaccaaaag catcactgaa ataaaaggga
    17761 aaaacagagc cataccgccg gctttaggta ttgcatgact catcgcatgc tcaatacctt
    17821 caacgtcaga catgatagtt tgagataaat ctgataaatt atgttttgaa aaataggata
    17881 atggtaaatt tgacaaatcg tctgcaattt gcactcttaa atgcgcactt tctttataag
    17941 tactattata taagttttca tactccctat ctaacaacca atacatgaaa agcaaagtaa
    18001 caaacgatac ggcaaaataa agccaacggg gctttgtatt ttctaaaact aattcgtcta
    18061 tcagcatcat tataatgaat gctggtacca tattaatgca gtatacaaaa aaactagaaa
    18121 tagaagcttt agttaaagct tctgcgccct gatcacttaa agcaaatttg tttttataaa
    18181 aacttttcat tgtcaagcct ccaatcattt gcattctcat aattagtgac caattgttta
    18241 tacacttgat gtttagccat taattcatta tgatttccac gttctactat tttcccttct
    18301 gacaagacaa gaatttcatc tacagattta atgctggata atcgatgagc tatcataata
    18361 actgtttttt cttttatcag atgttgaaaa gccttttgta gttcatactc attatctgga
    18421 tcaatagatg cactggcttc atccataata acaatctttg catcttttaa aattgctctg
    18481 gcaatagcta tacgttgttt ttctcctcct gacagataaa ctcctttact accaattaag
    18541 gtatgctctc tttcgggaaa tttttctaaa atcgattcgc aaccggctaa tcttagcgcg
    18601 tcaaatactt tacttgctgg cgcttgagga tttgctatag aaacgttatc gtaaatactc
    18661 tgattgaata gtttcgtatc ttgaaagaca aaagcaattg tgttaataat tgtgtctttt
    18721 gaatagtgat ttaaaggctt accaccaatt ttaatctgtc ccttatccac attgtagaaa
    18781 cctgaaatta gtttagctat cgtactcttt ccgcttcctg attttcctac taatgcataa
    18841 attctttttt gttctaaaac taagttgaaa ttttctaaaa cacactcttc tttgtatgaa
    18901 aagctgacat tttcaaatgc aatgtcacaa tttttaatct gtgtctcgtt cccatatttt
    18961 agttggtctt gcttcatttc taaataaagg gagtctagtg tttcgactgc atatcttgct
    19021 tcaaacaagt gcatacttac atacattatt ctcatgaaag aaacaaaaat aattccactt
    19081 aaaaaaaaca acatcaacaa gtcaacagct aatatcttgg gagtggtaag agaagttata
    19141 aaaaaggtta taggaactat caaaatgcaa actatgccaa aaaatagcca ttgaaaaaga
    19201 acaaaaggtg ttttcccact caaagaatac ttatatgcaa acttagcata atcgttaatt
    19261 gctttattca aggctttaaa tgatgttact ttagcaccaa atatctttat aacttgaatt
    19321 ccgcggatat attctaccgt ttcagagctt aacacatcta acgattcttg atatttttga
    19381 ataaaatttg tattctctgt cattttcttt aacaagaata caccaattac taacatggcg
    19441 attagaacca atccaatttt aaagttaatg aaaaagccta aggcaactga taaaacaggt
    19501 gttactatgg cttgagcact atctggaatc aagtgagcaa ttgccatatg tgttttagcc
    19561 gcgttgtcat caattgtctt tcgaatatat cccgaagaat ttaaatcaaa aaaacgaaaa
    19621 cttgcttctg ttaaaccgct aattcccttt tttctcaaat tagtctctaa gcgaaaccca
    19681 aggatatgag aaaataagcc agaaaaaata taaaataaag ctcctaaagt taaccaagca
    19741 acagtttgaa ccgcataact cttagctaaa tattcatctc ctacaataat tagagactga
    19801 agaaatttgt aaatactgta atatccatag actgttaata cgacagaaaa atcagaaaat
    19861 aaagtggcta accatccgta gtatttcatt ttagggacat aactaaataa ttttttgtat
    19921 actttcattt tttcctcccc cgcattcaat ttcttctctt attcgtttac aactgttaat
    19981 gctttccgta atagcgtaaa ttagagatgt acaaacagat aaaaggtaaa ctacgttcta
    20041 ttttttgtat ttttttaaga cttttcttcc aatcaataca ccaatcatag ataaacttgc
    20101 tacaactaac actgaaatga ttccccatat acctaccgca tagtggatca ttgcatccat
    20161 tgttgcagca gattgtcctt tatctatcca atcttgacga aaacgatctg taaagaacca
    20221 tataggaatc cactgtccgg cagcactgcc taattgcatt agagcccaag acgcaacttg
    20281 aggaattgct cgatcatatc caaatacaga tagaataata tcagctaaga tcgccatact
    20341 aatcgaactg ataaaccaag gtaagtacgc cccgcccatt aacgtaaaaa ataacaataa
    20401 aacaattgtg taaatagtaa aaattccttt tctagggtag ttatagctaa tataaacaaa
    20461 tatgattcca gataataatc caaaaatccc gggggatatg ctgtgaccaa acgccccacc
    20521 aatactacta accattattg aaaaaaagta aattactagg gaaataactg tagctaaacc
    20581 aataaaaata taatcttttg ttttcatata tattctcctc aattctattt cgttatgtca
    20641 ttaaaaatct gttctatcaa agttgaatct tctactctaa gttttatatc tttttttatt
    20701 ccaccatttt ttaaataaat aattctattg catgttttat aaagaaattc cggatcatga
    20761 gtagctataa ctactgcacc cttttctttt aagtctttaa taaggtcagc aatcctttcc
    20821 atattaatat aatccaaccc acttgtaggt tcatcaaata gaaataaatt agtttttgac
    20881 aatgaagcta tagaaaccaa taatctttgt ttttctccac cggataaatc aaacggtaat
    20941 tgatctttca ttttataaag ttccattttt tttaaaattt tttcttgctg atctttacta
    21001 actatttcat tccccagggc taactcgctc ctcactgatt ctgtgaaaaa ttggtaatcc
    21061 atttcttgca ttactaaaaa cggcgatgac aaattttttt tattttcgat tatctttcct
    21121 ttggtagggc gaataatctt catgagtatt cttagtaagg ttgttttccc cacaccgttt
    21181 ttccctacca aaccaataac atctccaact ttgacatcaa aatttatgtc ttttagaata
    21241 ttttcataag taatttcttc aacttttaat acatctgcac ttaccttttc gatataggga
    21301 acttcaatcg aactattaaa aatgtcaaat ttccttaatg cgttattgtt ttcagttttt
    21361 aacaactgaa tagttgtagt ttttacagtt ctatttttaa caaatagaac ttgatcaatt
    21421 aattctttca aatagtaaaa acgatgttca gctataacaa tagtaaatcc tttatttttt
    21481 aaatcacaaa gtaagtcagc aagtttctta gtcgctttga agtccaaatt tgaagacggt
    21541 tcatccaata taattaattt agagtttagc gataaactac atccaataga aattttctgc
    21601 cgttcaccac ttgatagcga aaaaatgttt ttatctaata tatttgaaag atctaataat
    21661 tcagctaatt cattgattct tttatccata tgctcttttg taaatccaaa gttttccatc
    21721 gaaaatacta actcactcgt tgtatttgtt gtaaagaatt gtcctctagg atcttgactt
    21781 acgtagccaa tattttttga tatactttct tgtgttaagg attgtagcga gttattttca
    21841 aataaaattt ccccaaccaa ttttccgtca taaagtcttg gaataagtcc attcattaac
    21901 tttaatagtg tggatttccc actcccagaa gaaccagtaa tcaatgtaag actgcctttt
    21961 tttattgaaa aattaaggtc aacaagcgct tgttccttaa catccttata tgaaaaagaa
    22021 acattgttaa attcaaacaa ttctttcacc tcaaatcaaa atacttaaga aaaaaatagt
    22081 aacggaaaac aggatgatat agtctaataa gctcatgttt gttttatagt aagaacttct
    22141 tttcacagga gattctatcc cctttgttat tgcattagcg gtcatttctt cagcaatcat
    22201 agttgcacgt ataagaacgg gaactaacag aaattccaaa tattgcagag gctgaaaaaa
    22261 agacaagcga atgtttctca tttttaaaga ttctttaatc attcttattt cagaacgata
    22321 agtcggaaag aatctgatca ctactgtaat acttaacatt atttttttcg gtaaatgtat
    22381 tttttctaaa gaggataata attcattgct agtatagaca ctaaccaaca accatgagat
    22441 cattacgacc ggcaacattc gaaatacaat atataaggat gtcgcaaaaa atatagtaaa
    22501 aaactctatc ttgatagcat caataatttt cattactaaa aacagaactg aataaatcaa
    22561 gatgaaataa atacatgtct ttattttacg aaacatcaat actaaaactg tagcaataat
    22621 gaaacacccc acttgaaata tatttccctt tcctaaaaaa ataatgaccg gaaaaaaaat
    22681 atttaaagta atcaaggtta acggattgat ttttgacact ctttcacctc caaaaaataa
    22741 gatcgcactg ttatcttaag ctgataaaaa tgcaccgtta ttcagataac agtgcgatct
    22801 tatcaaaaag cataactgag aacaattctc aatgcaagct ttttttaatt aactcagtaa
    22861 ttcaatccac ccaggaacag aaaattgata caatcttctg gttaatagca tagtcttttc
    22921 ttttgaatag ctatgcttca atatatcata gaatgtagcg tagaatgagt aaatgtagaa
    22981 gtggatttct tcttcattaa agactaaatt ttgattgatc attccgatag tctttaactt
    23041 atctagacaa cagactgtct ttttcgtcac aaatgatgta attttttcaa caatatcttg
    23101 atatttggtt ccagctgatt ggaataaaat caaatcgaat atacgtttat tctcaaaaat
    23161 ataattaata aatggatcta attcattgaa gttctctctc caacttttct ctatatctat
    23221 attttctaag tttaagaggt atttggtaaa ctcttcatta tacatattaa taagcgcatt
    23281 aatatgcggc tgaataattt cttcaaataa cgcttcctta gaagtaaaat gtttataaaa
    23341 agcacccgta gttacgcctg cggtcaggca cagttgcctt atcttagttt gctgaaatcc
    23401 ttctttttca aaatggctta gtccactttc tattaacttt tggtgagtta taatataatc
    23461 tctggtttct atgggtactt cctcctaata agatgaatta actttattat cttacacagg
    23521 acttgaagtt tcaatcgtta acgtttatta ctgaactcat tccttagagc taaactaatg
    23581 agaaagagca gaataactga tacaaaaagc aatcctacaa gaattaatag actcatctaa
    23641 atcattcacc ttttctaaaa taatccgaga aagatttaaa aaaattatat ccagctatta
    23701 ggataagtcc tatcataatc actccaatag ccattttctt atcctcccta aaaataaagt
    23761 cttttactta aaaccttttt tgtaatcgtc aataaatttt ttccctttat cagttaattg
    23821 aaatcctata tctaaatagt cctcatatct aagtttagcc agcatctcac ttacagatgc
    23881 tggactaaca cttagaactt ttgctaaatc attatttttt aatttctttt ttctttttga
    23941 taattcctga attgctagta aataaattag tatagtagtc gttttaattt gtaccatacc
    24001 taccctccaa attatttcga aaggccttca gcaattttat ccaggttcca tttcatcatc
    24061 gcatagtaac tatcaccatc ttgtcctttt tctgcaattg aatcagtaaa aatcgttgaa
    24121 tagataggaa tattggtatc ttttgatact gttttcattg gtctatcatc cacactgctt
    24181 tctacgaata aggagggaac ttttgttgtg cgtaattttt caactaagtg ttttatttga
    24241 tctggtgttc cttcttcttc agtgttgatt tcccaaatgt aagcagaagg cacattatac
    24301 gctttcgaga aatatttaaa gcatccttca cttgtaacaa tcattttttt atcattcgga
    24361 attgaagcaa atttagattt agcttctttg tctagagaat ccaatttttc aatatactta
    24421 tctagatttt ctttatagaa ttttttatta tcaggatctt tttccgctaa ctgtttttca
    24481 atatttttag cgtaaataat accgttttct aaatttaacc aagcatgagg atcttccttc
    24541 cctttctcac tctgaccctc taagtaaata acatctatgc catcacttgc tgcaaaatag
    24601 tctttgtttt cctctttgtt cgcattttta actaattttg taaaccaagc atttcctcca
    24661 gtttccaagt taacaccgtt ataaaaaatc aaatctgctt ttgaagtttt ttgaacatct
    24721 tcaggcaaag gttcatattc gtggggatct ttcccaatag gtacgatact gtgtaaatca
    24781 attttatctt ttgctatatt ttcggtaata tctgctaaaa tcgaattagt aactactact
    24841 tttaattttt cgttcgaagc cactttatct ttgtctttac tatttattga acatgccccg
    24901 aatataaaaa gagcaatcaa aatccccaga cttcccagta ctgctttcca ttttctcatc
    24961 ataacgatct ctccttcttt cttaaaaatt tttgttttgg tgagattaga aaactaatta
    25021 caaataagaa tgccgctgtt aaaacaatac ttgatcctgc tgctatgtta aaactataac
    25081 caataaacaa tccaagcaat gatgaacttg ctccgactgt tgcagaaata aatatcatag
    25141 ttttcaagct atttgcatat aaatatgctg tagctgctgg tgtaattaac attgcaacaa
    25201 ttaatatagt tcccacactt tgcatagctg ttactgctac taaagttaat agaaccatca
    25261 atagataatg ataaaaactg acattcatcc ctattgcttt tgccattaat ggatcaaaag
    25321 aagtgattaa taattcttta aaaaatagaa ctactataac caacactaaa accgaaacgc
    25381 caattgtaat ccacttatca atatcttgta cagctaaaat atttccaaat aaaatatgaa
    25441 ataaatccgt tgagctattt gcaactccta taaggataac tcctagtgct aaaaaagaac
    25501 tgaaagtaat accaattgct gtatctcctt ttattacact attgcttttt ataaaagtaa
    25561 taatgataga agctaataga ccaaatacaa tcgcaccaat aaaaaaatta atacctaaaa
    25621 tataagaaat tgctactcct ggcaaaacag catgagaaat agcatcaccc ataagagaca
    25681 ttcctcttaa aataataaaa caacctactg ttccagaaac aatccctata actacagatg
    25741 taattagagc attctgtaaa aagtgaaagt catacagacc attaataaaa ttttgaagca
    25801 tattattccc cctctccaat aaatatagta tctccatagg ctttttttaa attttcttca
    25861 ttaaatacag attctacact tccgtgagca attagatttc gattcaaaag aataatatta
    25921 tcaaaatact tctttacctt gcttaaatca tgatgcacaa ttagcactgt ttttccctga
    25981 tgtttcaatt tttttaacgt atccataatt attcgttcac taactaagtc tatccctaca
    26041 aatggttcat ctaaaaatat gaaatccgca ttttgagcaa gacatcttgc taaaagtaca
    26101 cgttgaaatt gtcctccaga taattctcct atttgatgat tagaatattt tcccatatcg
    26161 accttttcca aagcctttga aactctttgc cattctgcat ttttaattct ttgaaaaact
    26221 ttcattccgg catatgttcc catagataca cattctttta cttttattgg aaaagtaaaa
    26281 tcaatatcag ctttttgctc tacatatgca atcttcacca gttttttatt gatatcttca
    26341 ttattaatgg agacctctcc ctgatgaggg ataatattta acattgcttt aagcaatgtg
    26401 gattttccag caccatttgg accaataata cctgttaaag caccttcttg tatttttaaa
    26461 gaaatctcgt tcaatgctaa aaaatcgtta taacaaacat taagcttttt cacttctaac
    26521 ataactttct ccttctaaat gataatgatt atcacgatca tatggtatac aaaaattata
    26581 ttttagtcaa ccctaaattt tatagtttag atttttttct gttgctgaag attagaatga
    26641 caaaactatt tgaaactacg cgtatagtaa gtagctttca aaatatataa aaaaggagcg
    26701 ttctcaaatg agaacatgct cctaaaacta ctttattggc gtatggaaaa attatacttg
    26761 aagtggatag cgggttgaat aagttgataa gcatcaaaac atttataaaa attattcaag
    26821 actatgtctg ttccatatac aaatatgaaa atcagtaaac tccatttaac ttttttcgta
    26881 gtgtgtcttt ttatacatgt ttatcgttat taatagtgaa taggaaaaaa actatttttg
    26941 atcattgaca attaaataca atttttgaat aacgatccct gctattcgag ctagaaactc
    27001 aagtacgcca tgacctgatt agcgacaaat acttagagat ttctttttgt tctgggaaaa
    27061 caaaatgcca tgacaatagc agcaaataat gatacttccg taaggctcgc cgtaaactgg
    27121 ggagaggtga aattcctatg tgcttaccca aagcaattca aaaatttcat aactactatt
    27181 ttaggagatg attttataga atccagagtc tacctgaata aaaaacaaac ctgtgcattt
    27241 ctaggaatcg ataataatac tctagataag tggattcgta tatataattt cccagtaata
    27301 agagttggtg gtgtagttcg ctttgacgta gaagatatta aagactttat gaatacattt
    27361 aaaataactt gaaattttct tctcgccgca tatactaaaa ttagtgaaat atactgcctt
    27421 acttttttta aggagtgagg attttgccta caaagctttc aaatgggaaa tataaaacta
    27481 atctgagata tccaaaaagg tttaaagaaa taacaggaat tgcatcagaa aagtatcaaa
    27541 aaacatttcc aaataggcaa ttggcgatta aagccgaaaa tgatatgaag aagaaaatag
    27601 aaaaagtttt gagagaggag aatgcaaact cattagaatt aaaaggaaaa ataacgttta
    27661 aaaaatttta cgaatctaaa tggcttccta gatatgaatt aggtcaaaca attagatcaa
    27721 acaggcctcc tagtgatata accattagta atacaaaaga tatatttcgg ttgcatattt
    27781 tacctatgtt tggtgaatac gcaatgaatt atctaaatat caatactgag ataatatcag
    27841 atgaattaac caaaaaatcg aaagaatatg caaatataaa aatcataaaa ggatatgttc
    27901 gttccatgtt cgatattgca gaaatactaa attatatcga atttaatcga actactaaaa
    27961 ttattcaaag tatcactgca cctaaaaaaa atgctttaga agaaaaaaga atacaagaag
    28021 gtaagcaagc actatcatca aaagaactta ctaattggat agaagcagta aatgatgatt
    28081 ttaataatca tttacttact tttcatgatt acacactttt tatgattacc ctgtatcttg
    28141 gtgatagaaa aagtgagact tacgctttgc aatggaaata tattgatttt gaaaaacaaa
    28201 cggtccgtct taaacatact ttagataaat atcaaagaaa aaagtttaca aaaggtcgaa
    28261 aagataccgt cattcaagta ccagaagttg taatgacatt gctaagtgaa tggaaatcgg
    28321 tacaagctga tcaattgttg aaattaaaaa ttaagcaaac gttagatcag tatctcttta
    28381 cttatactaa accatctgga gaagtaaatt gtcctgttca tgccgattac ctaaactatc
    28441 gaatcaattc aattaaaaga aggcatcctg acttagttca tttatctcca cacaaactcc
    28501 gtcatactta tgcaactata gctagacaag gtggagcaga tatgaatcaa atttcaaatg
    28561 ctttaacaca ctcagatatt tctacaacaa agatatatgt gaacacacca gatatagttg
    28621 ataaggctgt ttttgaagct tttcagagag gattgaataa gtgtgattga tatgtgttct
    28681 taaatgtgtt cttaaaaaca aaagaagcac tttccaaata attgaaaagt gccagaaacg
    28741 ttgatatata aaggatatta acgttttgag aattgtgaag ctttacgtgc tttcttaaga
    28801 cctggttttt tacgttcgac catacgtgcg tcacgagtaa gtaagccagc acgtttaaga
    28861 gctaaacgga agtcaggatc aacttctagc aatgcacgag cgataccatg acggatagct
    28921 cctgattgtc cagcgtatcc tccgccgttt acgttaacaa atacgtcata tgcgcctttt
    28981 gtttcagtaa cagcgaaagg ttgattgatt acttcacgca agtcagcatg tggaatgtat
    29041 tcttcaacat cttttttatt tacagtaatt ttgccagttc ctggtactag acgtacgcga
    29101 gcaactgcgt ttttacgacg gcctgtgccg atatattgaa cttgtgccaa tgaaattccc
    29161 tcctattaga ttaagtttgt gatgtctaaa acttctggtt gttgtgctgc atgtggatgt
    29221 tctgctccac cgtatacgtt caattttgta aattgtttgc ggcctaaagt gtttttagga
    29281 agcatacctt ttacagaagt ttcgatcaaa cgacgagaat ttttagcacg caattcgcca
    29341 gcagtgattg atttcaatcc tcctgggtag ttgctgtgac ggtaataaat tttgtcagtc
    29401 gcttttttac ctgttaattt cactttgtct gcattgatga cgatgacgaa atcgccagta
    29461 tccaaatgag gtgtgaaagt tggtttattt ttaccacgta gtacagatgc aacaactgtt
    29521 gaaagacgtc ccaatggaac atcagttgcg tctactacat accatttacg ttctacttcg
    29581 cctggtttgg ccatatatgt tgtacgcacg ttattttcct ccaatttaag ttctgatgtt
    29641 tgacatacac ttcgagtttc cggggctctt tgtggggcaa acaataccat ttaacatatt
    29701 acccgctaat ctagtcaatg tcaacgtttt tctgcttgat tgaaaaattt atatgaaact
    29761 ttacttcttt tctttctttt atataaaagg gtcgtttcct gtcaaaatga aaaacaaaaa
    29821 gaggctgtga caaaagtctt gtcacaaccc cttattttga ataaacggtg ttctcctgcc
    29881 aactcctcgg tcacaagtca caatatccat aaaacataag aagctgtttt ttaaaattgt
    29941 ttcttgtgac tcggagctga acggcaggct tacaacctct tggtaaacgg tgttcaatca
    30001 gctgaccctc ggtattagct caaaaaacgg caaaatatga ggaactattt tgacgtttgt
    30061 ttctctaata ctcaggtcaa aacgctgatt tcacaacctc tttttcggtc aagttcaaca
    30121 acagttttta cgcgtcgatc tctggcgctt tgcccaaatg agcttggatc atccagatac
    30181 gtttttctgt ggctgttttg aattcgataa agatatcttg tgttggatcg tcgccttctt
    30241 ctccagaaac ttcgattcct ttttggtaaa gatctgctag ataacgatag cctgcaacaa
    30301 gtgtttccaa acgttctgga atagtacgat cccagcggcc aatttcatca gggatttttg
    30361 tgttatctgc aaattcacgc aatgtagaat aaggtgaacc gtctaatgtg atcaagcgtt
    30421 cggatacttc atctaactga gcgttcaaat catccatgaa ctcatccatc attgggtgca
    30481 atgtaaggaa acctggtcct ctcatgtacc aatgtgtttg gtgaacaaca acagacattt
    30541 ggcttagatc tgcaactaat tgatttaata cttctttggt ttgttggaat ttcatctttc
    30601 ttgcctccta acattttctt acacttaaaa taatagtaga ctttcaatga aaagtacaac
    30661 aaaatcattt acctgagaac gattttcagt tcgctttttt atccgtaaag ccaatcattt
    30721 ttcagaatca gctaattctt ctcgcagttg agcaaattgc tgataagtct gtgctaaatg
    30781 cttttgtgtc gaaacaaata atggatagat tttctgatat tttttatgat cagtttcttg
    30841 tactccaact tgctctttca attctatcaa ttcctgtgct tcactgatac tttggatctt
    30901 tccgatgctt ctccagccta aaatcacagc tcctaatgcg gatgcttctg tgacagtagg
    30961 gaaaacaagc gggtaaccca gtacatcagc catcatctga cgaaaaatcg gagattgagc
    31021 aaacccgcct gtagcacgga tctctgtgag ctgattttcc tggcaaactc cttttaaaat
    31081 ccgataaagg ttgagacaga tcccttctag aatcgccttc atcatttctt tttctgtatg
    31141 ttgccgctgt aaaccgataa aactgccaaa cgcatctgcg tcccaaagcg gtgctcgctc
    31201 accaagtaaa tagggatgaa aaatcagacc attggcaccc gctggcacaa aagcgatagc
    31261 cgaaagcagt gcatcatatg gattttctcc tttttcttta gcagattgaa cgacttcctg
    31321 taaaaatgta tgccctgccc aatcaaagat gccagcacca ttgctagtcg ctcctcccac
    31381 tacccaatgt ttctgatcca gaacataaca aaacgtctcc atttctggat ggaactgagg
    31441 ctgagtagta ataaaacgaa gtgctccgct cgttcctacg gtcaaagcgg cgatcgtatc
    31501 accaaaagca cctaacccta gattagaaag cggcccgtct gcccctccca aaacaaatga
    31561 tatatcattt ggcaaaccta gttctgctgc tgtttttgat ggtagacctg ttacttgata
    31621 ggtggaagga aagacttgcg gcaattgctc tattcgaatg ttcaagtagt caagcacttc
    31681 ttctgcccat tggaattcat ggatatcaaa atatccagtt cctgaagctg ttccgtagtc
    31741 gctgatcagt tcgccaaaca atctataaaa gatatagtct ttgataccga caaaataagc
    31801 agtttttttg aataattcag gttggctgtt ttgcagccac ttgattttgt tcaatggtgt
    31861 cattggatga atcggcgtcc cagtctgccg ataaaattct ttgccaagca cggtatattt
    31921 cagtttttct gcttcgtccg ctgctcggtt atctgcccaa gtgatcattc gagataacgg
    31981 tgttttctct tcatccaaca atatcaggcc atgcatcgca gaggagaagg agacaaattg
    32041 aatgattgtg tctgaaggca actgtttaat tacttctttt ataacggtgc agacaccttg
    32101 aaagatcaat tcctgatctt gttctgccat cccttgtgtt tcttggatca aagggtagcg
    32161 aaaataagcg gatgctgctt cttgggcttc atctgtgaaa gcaactgctt tggtttgtgt
    32221 ggttccgata tctaatccga ttgaaacgtt cattttttca cctcaaattt tatgtatgta
    32281 caaaaattta gtttaagctt gcgtatcttg gttttgaaaa gggggagatg gcgtgttact
    32341 ttattttatc attggttgct gtatcggatc atttttatgt ttaaccgccc agcggattcc
    32401 gttaggtcac tctattattt atcctagatc tcattgtgtc aaatgctgtc gttcgctttc
    32461 ttggtatgaa ttgattccga ttctctcgat catcgtccaa cgatttcgct gccgctattg
    32521 caggtgtcgg ttgcccttct actatttgtt ggcagaagcc ttatgtggtg gtctttttgc
    32581 ctggttcttt actttttctt ccgcaagaaa tttttcgacg ttcatatgga tgctttcggc
    32641 tttgctattt tcgttgatgg atttatttta cttcatggtc cattctcaca cgctattctg
    32701 ttcttgggca attttatgga ttttttggtt gcaggccggc gtcttccaat ggcaaagtgt
    32761 gctgttaacg tttatcgtag gcggtgtgtg ccttcgctat ggtcaatcct atctcggagc
    32821 tggcgatact ctgttgatgc tttcttggag tggtggcttg tctctagaag agttgcttca
    32881 gatcttgttt cttgctagtc tgtttggaat atccgttttt tctgtttact ttatctgtca
    32941 taaaaaaaag ttggaaaagc tgcctttcat tccttttttg agcagtgcgt tgctgattgt
    33001 ccttcatctt aggtattgaa aaaaggttgt gacaaacgac ttgtcacaac ctctgatata
    33061 tagtgttctc ttaccaaatc cttgtgactt ggagctaggc gacaggttca caacctctcc
    33121 actaaatggt gttcaatcag ctgaccctca gtattagctc aaaatgctgg ttttacaacc
    33181 tctgatatac ggtgttccat aagtagcttt ttcggcttct tatttcttga cgccccaatg
    33241 tcgacgaagg aaacctgcac gtccagaacc ttctcgataa agattgtaat ggagcttgtt
    33301 tttcttgtaa taatcctgat ggtaatcttc tgcaggataa aattcgcttc ttggttcgat
    33361 ttttgtcacg attggcttat cgaatcgccc gctgtcttgc aatgcttgtt ttgaacgctc
    33421 tgcaatattt cgttcttctt catcattata gtagatcact ggacgataag aatcaccgcg
    33481 atcagcgaac tgtcctaatg cgtcagtcgg atcggtttgc tgccaataga tttccaccaa
    33541 ctgatcataa gaaataactt ctggatcata ttcgatctct actgcttctg tatgccctgt
    33601 tgtgcctgtt gttacttgtt cataagttgg atttggtaca tgcccgccag tataaccgga
    33661 tgtcacagaa atgatccctg gctgctcttc aaatggtttg accatacacc aaaagcatcc
    33721 gcctgcgaaa actgcttttt ttgtcatata aaatccccct tttttctcat cataccttga
    33781 tttccaaaag gaggataacg ttttgcttgt gacagaaaaa acggaaacta ttatatacgt
    33841 gatacggctt ctgataaatg ccaaaacgaa cgtacttgat caaaaacggt taaatcagga
    33901 atcacatatt cagcagaagg aagatttatg ttttcaccta ttcccaatac atgcatgtta
    33961 ccagccaaag ctgcttgaca accagcttct gaatcttcaa tgaccaagca tgcatttggc
    34021 ggtacattca attgctgtgc tcctttgaga aaaacttctg gatcaggttt tgcttttgat
    34081 acttgcgtgc cgtcaataag gacatcaaaa taacttgtca aattcgtttt ttctaaaatg
    34141 aggcgtgcat ttttgcttgc tgatcccaat ccgatcttga tgtttgtttt tttcaagtac
    34201 tcaagaacag ctattgcacc aggcaaaacg gatgtttcat ccattttttg aatcgcttga
    34261 agatatagat tgtttttttg ctcagcaaag gctgatttct ctttttctga gaatttctgt
    34321 tccatttttc caaatgacag caatcgctct agtgactcgg ttctggatat tcctttcagt
    34381 tgctcgtttt ctttctcagt gaatggaatg gacagctggt tagctaaaac cagccacgct
    34441 tcgtaatgat aacgagctgt gtctacaaga acgccatcta aatcgaatag tgctgctttg
    34501 attttcatcg attacctcct tgaaaatgaa cgacttgaag taaagatgcg atgtttttag
    34561 ctgtgcgatg aagattatca gtggcttgct gtaaagcttc ttgtaaaggc acagctgcag
    34621 gaaggatcgg aaaaacggcc gaaatgccaa catctgctgc tttttcgctt cctttaccaa
    34681 cacttccgca aatagctatc acaggaacgc cctctggtac tcttctagca atcccaactg
    34741 gcgcttttcc ttttagactt tgtccatcta gacgtccttc gcctacgatt acgaggtctg
    34801 cttgctgtac ccgtttatcg aactccagta aatccaatac gtagtcgatg ccagagacta
    34861 ttttccctga agcaaatgct aacagtcctg cacccattcc accaccagca ctactgcccg
    34921 atgcctcgat tacttgaggg gcaaatctca tataaaaatc atgcatggct tggtcagcca
    34981 ctgctaattc attttctttc aagccttttt gtggtccgaa aacaaaagaa gcgccttctt
    35041 ttccgcataa aagattagta acatcagtaa tgacagtgat tttggcttta atcagctcaa
    35101 taggcacgtt cgttttatca actgtagcaa ttttacctag attttctccc agtgcttcta
    35161 ctttttgatt atcttgatcg taaaaagcat aacctagacc ttctgccatg ccgatgccgc
    35221 catcgttggt actacttccg ccaacgccga tgatgatgtg tcgaacacct tgttgcaaca
    35281 attgtcgtat cagttctcct actcctcgcg tggataacct aagtggctgt tgttggttta
    35341 gtgggacatc tttcaatccg acgatttctg ccatttcaaa aatcgcagtt tgaccgtctg
    35401 aagcgtactg gatttctttg tttttgccaa atgcattagt aacgactgct gtttgccgct
    35461 tcaaccttaa actgtctgct aatgatgaaa ccgttccttc tcctccatcg cctataggta
    35521 gcagatcata ttcagcctga acaaaaactt cttgaaaacc tgattgaatg gcttgtgctg
    35581 cctctaaggc tgtcagactt tctttgaaag aatctggcgc aatcagaata cgcattattt
    35641 tcccctccgt tccaagatca gttctttttc cagctggtat tggtctccat tgacaaaaat
    35701 aggtaatgtt tctttcgtct ctttttttat tactatttgt ttttttgtca aaacaaatag
    35761 caacctgtga tttttccaaa taaaaggaaa agaaattttt tcccatgctg aaggaagtct
    35821 tggagaaata gaaagagttc cattcgttac tgtaatacct gaaaaaccaa atataactgc
    35881 tagccatagt gctcctaatg aagcagcatg aattccgtca tctgatgaat gcggatctgg
    35941 tcctaaatca atcaggcacg cttcttggaa taaatgatac gcccattcca tttcttggat
    36001 acggcaagcc tctatggcgt gaatcgcttt actcaacgat gagtcgtgga ttgttttgtt
    36061 ttcatagtaa tgcaaattct tagaaacgat tggttgagaa aaatactcag gtaataagta
    36121 taacagcatg acaagatctg cttgcttgct gacttggagt tcatttactt cgctacgggc
    36181 ataatcaagt aaaatagact ggctgccagg acgttctttg taagtggtca aatcaataac
    36241 tggcttactg agatacgtgt catcttgcgg aatcagctgt tctttgttag gcactggcaa
    36301 atataactta tctaaaaaat tttgacattc tttcaaaaaa agcggatcgc cagcttgata
    36361 tttaacagct aaggccacat tataatgcgc cagatagttc gtatatgcat tattgtcgat
    36421 atattctgtg tattcatctg gaccaatgac tctgtgtatc tctaaatgat catcgacttt
    36481 tacagctctg ctcagccaaa aagctgctgt ttcatgaatg agcgtttttc cctttgtttc
    36541 taaaaacgat tgatcctctg ttgcttggac atactcttga acggcataag caaggtctgc
    36601 actgatatga tgttctgcgg cagctgaagc aacttcttgt cttgttccgg ttttgatatt
    36661 caatgcagca aatttgggtg tctcttcctg accagtgtgt gcgctttccc atgggaaaag
    36721 tgcaccttga tagtgataat ctcttgcttt ttgccgtgcg ccttctaatc gatcataacg
    36781 gtacagcagc aaattctttg ctacttcagg aaactgatat aaaaagaaag gcaggataaa
    36841 aatttccgta tcccagaaaa catgcccttt ataaccttcg ccagttagtc ccttcgcccc
    36901 tacactaaca cttggatcat tgcggggagt cataatctgt aaatggtaag cagcgaagtc
    36961 catcgctaac tgatcgaaag aagaatcgcc ttggatcgtt atccgatgct gatgccaaaa
    37021 atctgtccaa taagacatcg actgctctaa aatttctgta taactaacgc ttttctgggc
    37081 ttgggcaata cttttcttaa acgggtctgt ttgctgaaga gaggtagaga gcacgatgat
    37141 cttctccagc gtaaaggacg tattttttct gatcttttct gaaaaggaag cggtgatccg
    37201 gcgatttttt gcttggaata cacctgcttt attgaattta gctgcaatcc caatatcaat
    37261 tttactttct gttgtctggt aggatgcact gatcaaatcg tcaccaaata ctcgcagttc
    37321 tttttcaacc aactgctgtg taccaaaatt ggtttgctgt ccatcgatac ccgttttcac
    37381 atctacttgg cagtcttcgg tcaacggctt gatgaccact ttcagggctg cctgatggag
    37441 attattttga tcagcaacac gacaagtaat gacttccact ttttttcctt gagcagtttc
    37501 aatgagcgca tagcgtttaa gctctcccat ttctaaatct agtgcccgct cgtatgcaag
    37561 aactttcatc gtctcaagag aaaaatgttc accgtcgatt tttatttccc atctgatgac
    37621 atctgggaga ttcactaact cggctgattc tgctcctaaa ggtcggttat atacaccagc
    37681 tgcaaacatt ccccttactt gattccgata gtcttcctcc tggcttcctc tgataccaaa
    37741 aaatccattt ccaatggtca tctgactcgc atacttattg atatactcta aatcaaactg
    37801 tttttcttct actttcataa ggtaataact tctcctgttt ctgccgaacg atataaattt
    37861 tccaccagat tctggatgac atatccttgc cagccatctg caatcatctg agtctggtca
    37921 ccgagcgccc gattcacaaa atgagccata cttagttcat gtgggtcatt aactggcgtg
    37981 gtcttttgta aggaaaccag ctttccatct acatcttcga atagttcgcc tggatacaga
    38041 ttagcacctg cctgatcgcc acaaaaagcc acattcagtt tgtcttctct gatattcaaa
    38101 gcaaacgaag tattcagttg gatgatcttg ccgttttcta attccagtgt gccaaaaatt
    38161 gcatcttcta ctgtgaattt gcttggatcc catttcccga atttccctga attttttttc
    38221 gtaccaattt tttggaagct gtacgcccga acgcttttta ctttaggaaa tccagtaata
    38281 tacatcgctg tatcaagcat gtgaatgcca taatcgatca aaggcccgcc tccttgaagt
    38341 tctttgtcga taaacgttcc ccagcctgga acaccagacc gacgaagcgc attcgcttct
    38401 acgtaataaa tatccccgag aacatcaaga tgattcttga tcattgttgc ttcgtcagaa
    38461 aaacgatgct ggaatccata agccaaaatc tgcccgtttt tatcggctgt ctctgccatc
    38521 tttcttgctt gatcagctgt catagctggt ggtttctcgc acatcactga agcattagct
    38581 tcgagggcag ccaatacatg ttcgaagtga aagcgattgg gtgtacagac actcacgata
    38641 tcgggagaaa tctcattcag catttgtgta actgatgtat acgcatgaga aaatcctgta
    38701 cgtcggcaaa agtcttctgc tcgctggctg actggatcaa ccacagcggc caattctaag
    38761 acatctgatc ttttttgata ataagcagca tgtacttttt ctgctacttg tccagcgcct
    38821 atgattgcta ctttcttcat ttatttctcc ctcctttgct tatttactta ttgttattct
    38881 ttctctattc tttctctaaa tcatttaaat gacgcgctgt ttccagacag tttttcaagt
    38941 attgcaatga ttcctgataa attttcactt cattttctcc tcgcaaacga cattcatata
    39001 ccatataacc ttcatactca ttttgaagta gcgtttgaaa atggcgagta aaatcaattg
    39061 cgccagatcc tggttggtag cgatgattat ctgctaaatg gatatgaccc acatactctt
    39121 tttgcgtagt caatgctttt gaaatatcat cttcttcaat attcatatga taaaaatcag
    39181 cgatgatctg cacatgtttc aattgattag agtcaatgta atgacgcgca tccgctaaca
    39241 aattgatcat atggtcttgg tatcggttca aaggctccaa ataaataacc gtacctgttt
    39301 cttcggctac tttatctaaa taacgaagtg attcgctgac tgctttatag tctccttctt
    39361 tgcttcgggg tgaaaccatc ggcggtaatc gataagtgaa cattccccaa gctgctggga
    39421 taacgattcc tttgcctcca acctcgctta gtgcacgcaa aatctcagca atttgacgta
    39481 caccattaag acgccgttct tcgataaagt caccgatcca cccatcgtag ccgttacaag
    39541 ctgtcactac tgggatccct gtttctttga ttgcctgttt gactgtgtcg atattttcta
    39601 ttaacaactt accgtcgatt tcgtattctt caaatccaca gtctttgata aatctaaatt
    39661 tttctaaaat gtctcttggg aaaaatgctt gatcttgcgt accgaatttc attgttctcc
    39721 tcctcggact tgaacgccca ttttgatact ttcttctggg tgctggtcta cataacgcat
    39781 gtaagcttct gcgctttctg caaatggcac aactggatca atgatttctt cacaatccaa
    39841 gtaaccgttc atcaataatt cccaacatgt gtcctcgatt cgtttacggt tccatcttgg
    39901 gtaatctggg tttggttcac tagatgctcg agcaaagacg atttttgcat tattgaaatg
    39961 tgcttcgcga ccaagattaa acccagctgg aaaaggttta gcaaaggcaa cataagaaat
    40021 gatcccgcca tagccgatcc ctcttaaaga agcttgtaat gcggcagcac tgccactcgt
    40081 ttcaataact gaatctactc cacgtttatc tgtcaattcc tttagacgaa aaccgacatc
    40141 tgctttcata ggatctagcg tgtaatcggc accgtatttt tctgctaatt ctctacgctg
    40201 cgctatggga tcgatcccaa cgatggtagt agcacctgct tttttagcta attgaatcgc
    40261 aatttgcccg atagcgccta aaccgaagat tgctacagca tcacctgctc gaacatgtgc
    40321 ttcccggata ccacctaaag cgaattgagc aggatcgtaa catactgcat ttttccattg
    40381 atccaaccga tcaagtttac gtagtttata attatcaaca ccgtttgtaa tcagcgtttc
    40441 acgaatgggt ccataagtgc agactaactc tcctttttga tactctgtca ccaaactgcc
    40501 cgtttcttcg atcgtaccga caatcatatt tcctaattga aattcgccaa ataccacacc
    40561 gcgttggctt ccttcttctc ttggcaagaa taggcgccat tcttctgaaa attcttcatc
    40621 gataaatgga cttaatcctc taaagtcgac aatttctgtt cctggttttg gagtagcgaa
    40681 aattactcgg atacgtactt cgttcttttc gatctcacgg tcttcgtaat ctttgaatcc
    40741 ggctattctt ggttctaatg caactaattt tttcatcttt tctttcctct ttcttttctt
    40801 tttatccttt tacgccgcct tcgactaaat cacctttaat aaatcgttca gataacgcat
    40861 acataatgat gactggtaaa gcggtcacca atgaagcggc catcattcgt ccccaaatgt
    40921 agtctggtgt actgaataat gtgttcaaac cgattggtaa agtaaatttt tcggaggtcg
    40981 acaggaaaat cgaagcaaat aaataatcat tccacgaaac cataaagcaa taaacgaaga
    41041 cagaaatgat tccagaaata gccaatggta taatgatccg aataatgatc tggatccggc
    41101 taagaccatc gatcattgct gcttcttcaa tatcagtcgg aatcgtgtca aaataacttt
    41161 ttagcatgaa tatcgcagta ggaagtgttt gaacgaccat cgtgataatc agtgctgtat
    41221 tggtatcata caaacctaga ccggaaatga ttttaaataa tggaacaacg atcaaaatac
    41281 cggaaaacat ataaacggtg taaaagctag cattgatttt cgagcgagct ttgaagcgta
    41341 gttttgacaa agcgtatgcc ccgctgatcc caatcactac tgcagttacg gaggctatca
    41401 aggagatcac taaactattt ttgaaataca tgatgaatgg aaaaatctca gcattgaaaa
    41461 tatcctgata atgttcaaaa gtccattctt tcggaaaaat agtttgtgtc gttgcaattg
    41521 cttcttgtga atccttgaat gatgtcatca gcatgatcaa aaatggaaac aaattgacag
    41581 ctaatagcaa caaaatcgtc gcgtaaaata agattttcct gctcttttta tttttcattt
    41641 caagtccacc ttctttcgtg agagcaagac gaccgctgca ataatgatga acaatagaat
    41701 cgatatagct gctgcttttc caagatcatt gaacgcaaag gctgtctgat acaaataaac
    41761 gcctaagata ttgactttat tcgtcagtag atagacatct gtaaacatat agaacatcca
    41821 gatagatcgt aaaacaatca cagttgctag tactggtttg attgccggca gtgtcacgat
    41881 cttgaacttc tgccaagcat ttgcaccgtc catttcagct gcttcataaa gcgttttgtc
    41941 gactgtttgc aaaatagcca aaaatgaaat aaacgcatac ggatagtatc tccagatagc
    42001 aaacaaaacg accataaaga agctagcaac tggctggtcg aaccacagcg gtgcttcgtt
    42061 gaaaagctgc aacctatcta ctaacagatc attgacgatt ccataactat tattgaacat
    42121 atatttccaa gcaaaaatca aagaaataga tggcgtaaca taacttaata aaatcagtga
    42181 acgcaccgtt ttcctaaatc tgaacttgcg gttaagcaaa atagctacgg ctaatcctaa
    42241 agctgtactg cctgctacaa ctaagaatgt gtaagcaact gttaaaccta aagaacgata
    42301 aaattcacta tcacttaaaa tttcaagata attgcttaat ccgataaaaa cggcgtctga
    42361 gttaggattc agcggcatat cgaaaaaact gatctgaata ttggaaagca ttggaatcag
    42421 cactaaaaca gctaataaaa gcatgcttgg tgctaaaagt cccatggcta attttaagtc
    42481 agaatgtttc cttggacgat tcattgaaac ctccccctga taatctgcca atagctatct
    42541 acttttttcg aacaccattg gcagaacttc ttgttatttc gtattttttt gtgcgtcagc
    42601 taaagcttga tcgacatctt cgtttccgac agttacttgg ttcactgctt ttccgatcac
    42661 acctgagctt gtgatatctc ccattttcgt gaaattcttt tccccgacta gtccaaagac
    42721 ttgtacttca tcaaaagcac tagcaatctc agatggcagc tcgccaaaag acttgatgac
    42781 ttcattttct ttgtaaccat ccagttcgac tacttgttta ttcactggtt gagcaccacc
    42841 aggagacatc aagacccact tttccatgtt tttcggttga gataagaaag ctgtgaatgc
    42901 ttcagcagcc tctttttgtt tctcgtctag accagaagaa atcgtcaatc cagatactgt
    42961 gccatagaca gcttgatctt tttgtgttgg gatagcaaaa ccgatatttt ctgagttccc
    43021 cgcttcataa acagaaggca aaatgtaagt tgaatagatt gccattggtg ctgttccatt
    43081 catgaaagca tctttgattt ctgtcacatc attagagcct ggcatcgtgt attgggataa
    43141 gttttgataa aacgacaatg cttctctcat cttttccgta tcgatcgtta cctcgccttc
    43201 atcatccaaa acatttgctc catttgacaa agcaaattga gagaacgctt gctcactcat
    43261 tgttccttca gcagtaggca tagcaattcc atattgttta ttctctttat ctgtgaaatg
    43321 ttgagcaatt tttaaaatat catcccaatt ttctggttct gaaaaaccag cttctgataa
    43381 tttttctttg tcataccaaa taccttgaac ccagccgcta attggtgcag caatatagct
    43441 ttttccatct tctgaacgaa caaggttttt ggctccgtca taataattat cttctccagc
    43501 ttcctcgata acgttttgga ctgccgtctg atcaatcagt tcatctttat ccatcacttt
    43561 agcaaaatcc tgactgactt ccattacttc tggtaattta ccggaacgag ctaacgtaac
    43621 gatttttgta ttgaacgctg attcttctac cggcacttgc ttgattttga tgtctggatt
    43681 ttctttttca aaatcagcaa ccagttcatt gatcacactt aatcgttcct gttcgacaga
    43741 agagtgcatg aattgaatcg ttgtctgttg tgattcgtta gagctttgac tttcaccaca
    43801 agcagtaaga agaaatccag tagctagcag acaggtagta acaataattt ttttcatttt
    43861 tttccacctc ttattttatt attttcagcc acatatattc atacggctgg aatatcaact
    43921 ttcgttcttg actgcaatgg ataatttgtc cagaaaacag gtctaggtaa tctccttttg
    43981 gtaaaagata acctgctgat tgattggaaa aattatggat aactaagact ttctcttcct
    44041 tcgcttcgcg aataaaactg ataaaagaat ctgctgagtc cagtaagtaa atcaaactat
    44101 tcggatggaa ggctttttct ttttttcgaa tagatagcat ctgggtcaat tcaccatata
    44161 ctgctgttct taatggatct gtctgcaact ctttctcaat atctgctaac aaaaattttt
    44221 gccgattgat ttttcgattc tctcctgttt tttctacgcc tttgtaatca tttcgacttc
    44281 ccagcacact ttgaatataa attgccggca cgcctggtaa cgttaccaaa aatgaatgag
    44341 cagctaaaaa gcgtttgctt ctccattccg tcgtatctgt tggagaactt aacgcatcta
    44401 gatacgtagc gtttatctca tatggactgg tcgttccatc tgggtttttc ttgtagttta
    44461 ctcgggcacc ctctttcttc agctgttcca ctaattcaag gatcgttttt tccggcagaa
    44521 tacctcgtac cggattcaat ccgataccgt catgggaggc taaaaaatta aaaaaagttg
    44581 tttttttcga tgcgaaatcg atggttttta accattcttt caacttactt gtattacccg
    44641 aacgaatcgc ttgaagggtc aaaggtggta gcgaaaactg atagaccatc tgtgcttcat
    44701 tctcgccatt cccaaaataa ctgatattat ctttgtgtgg cacatttgtc tcagtgatca
    44761 ataccgttcc tggagctgct tcatcagcga ttgcacggaa taattggatg attttatgcg
    44821 tttctggcag gtgtatacaa gaagttccta gttctttcca taaaaatccc acagcgtcca
    44881 agcgaataaa tgaagcgcct ttttctaagt aaaacaaaag aatatcgatc attttcaata
    44941 aaacttctgg atttttaaaa ttcaagtcta cttgatcttc gctaaaagtc gtccatacgt
    45001 gtttttcttt cccgtttttg tcaatgaacg ttgtaagtaa cgggctggtc cttggtctag
    45061 tcacgctgct tgtatctgta tccagtggca aatcaataaa aaaatctttg tacgtatcat
    45121 tcaagtttaa atattcctga aaccaagtac tttcggcaga catatgattg cagacaaagt
    45181 cgaacattaa ccgtgtttcg cttctcattt cttctatatc ttcccatgtg cccaaacggt
    45241 cgttgattgc ctgataattt acaacggaga aaccatcatc agatgtatat gggaaaaatg
    45301 gcaagaaatg tacaactcta aatgtatctg ccaaatactt ttgataaaat ttcttaaatg
    45361 tacgcaaagt tggttgctgc tcttcaaaaa attgatcccc ataagtaatc aagaaaatat
    45421 cttcttcgtc ccaatgcagt ttgcctgcct ggcctttctt ctgctgataa acactgactt
    45481 tttgttcaat catagaggcg gttttctcag acaaatctga gtcgcgataa atcgcttgta
    45541 aaagttttgt atacatcgga ttcccctttc ttttttggga gcgttcccaa cgatttgatc
    45601 atattatgga aaacgcttac tgtcaatagc atttttttaa ataattttga tatacacaaa
    45661 aatacaaata agaaagctta tgtatcaata attaatagct tcttaaaaaa atatggtctc
    45721 tcatgatcat gcaagctatt cctaaatttt caaaatcata gacaaaaaag aatgtctatt
    45781 gtactatgga attattctaa aacgctccca aaaaggaggt gcatataaaa ttgaaaccta
    45841 ctatttatga cattgcgaaa aaagctaatg tctcaaaatc aactgtctcc agagtattaa
    45901 ataaccagcc aaatatctct gataaagcaa aagcaagtgt acttcaagca atcaaagaat
    45961 tgaattatga accgagcaaa attgcaagag gactgtcagg aaatggtttt gacgcgattc
    46021 tagcattatc tcatcgtact tctcatacga cctatggcaa tccttttttt tcagatgtta
    46081 ttcaatccat ttctgccgtt tctgaacaaa ataacttcga cttgattcta caaacggcaa
    46141 aaacaccaga agaagagcta gaaaaatgcg cgaaaaagat ccaagaaaaa atgatccgag
    46201 gaattattat tttaagctct acgatggatg aaacacttct ttttgaattg gatcaataca
    46261 atattcctat tgtggtaatc ggaaaagtcg gaaacgacta cacgaacatc tactcagtgg
    46321 acacggataa tttccaggat agttacgatt tggtggaaac actgatcaag aatggacacc
    46381 gtcatatcgg ctgtctccat gctccgacag aagtttatgt atccaatgat cgtctcaatg
    46441 gttaccgtca atgcttattt gatcaccacc tagatatccg taatgaatgg attatagacg
    46501 gcggctttcg cttagaagat agcatggcag caatgaatga tttaatgaag ctacgagaaa
    46561 gaccaactgc gattttttct accgatgcat taaaatcatt gagtatttac aaagtggcag
    46621 aacaatctgg aaagcagata cctgatgatc tttcgatcgc aggattcagt gatcccttat
    46681 tgtcctccct attttcgcca cagttgactc ggatagacat tccgacaaat cagttaggaa
    46741 tcaaagccac agaactttta ttccaactaa tccaccacca ataccctgaa gaaaaaagaa
    46801 tcattctttc aaccgagatt actctcacag attcgatcca acaacaagaa atgtagaaca
    46861 gaattataaa agaacagaaa cactgaggat aagtcagtta ctctgttctt ttttcaatca
    46921 ctcatccctc gttaccccct cccggtattt tagcgaattt ttcttatttt ccatctgttt
    46981 tagatagctg tggataacta taatgtttgt gttaaactat tttttccaca gggaagaaaa
    47041 aactattttt aaccacgttg tgtataagtt tttatagttc gtttttttaa acacatttac
    47101 ccttatttca atccatgtta tttggggata cttatctcta aaacataaat agaataacag
    47161 acatgtctac tggttttgtt ttattcgagg aattattcaa taatagctag caaaatatta
    47221 aaaataaccg ctctatcttc ccatcaattt attttgcatt caaaaaatga tctatcttta
    47281 cttttgttga ttgacttctc ttggaagatg atcgttccga taccttaatt ggatacgaac
    47341 cgatgttgtt aagatccttt cccacttttc ttctagttag acaaattatt ttagacaaac
    47401 caaaaaagag gttgtgacaa taatttgtca caacctcttt tttaaaatat ctattttgtt
    47461 tgattgttca ttgcgtcttc tgcagccatt tgcgcttcaa tatctgaaat actgtagaca
    47521 ttttcgcttg ctacaccgac ttcttttggt tccatgtcac gataacgttg cataccagta
    47581 ccagctggga tgatcttacc tataataaca ttttctttca aaccaagaag aggatctttc
    47641 ttaccgcgga tcgcagcatc cgtcaagaca cgagttgttt cttggaatga tgcagcagat
    47701 aagaaactat ttgtttctaa tgatgctttt gtgataccta gtaagacagg tcgagaagtt
    47761 gcaggaacgc ctcctgagac tagcgtattg tagttttgat ctttaaagtc tgcgatgtct
    47821 aataatgtac ctggtaagat atctgtatca cctggatcca ttacacgaac tttacgcagc
    47881 atttgacgaa ccattacttc gatatgcttg tcgccgattt ctaccccttg catacggtaa
    47941 acacgttgta cttcacgaag caagtagttt tcaacagata atacatcgcg aacttgcaac
    48001 aattgttttg gatcgattga tccttctgtc aatggtgcac cacggtggat catatcgcct
    48061 tctgctactt tcatacgtgc tgtataagga acagtatacg tacgtgtatc tgtttttcct
    48121 ttgattgtta cttctttggt acgtgttgca ggatcttctg agatatcgat gacatcccct
    48181 gttacttctg tgatcactgc ttgccctttc ggattacgtg cttcaaagat ttcttggaca
    48241 cgaggaagac cttgagtgat atcgtctccg gcaaccccac ctgtatggaa tgtacgcatc
    48301 gtcaactgag taccaggttc accgattgat tgagcggcga tcgtaccaac tgcttctccg
    48361 acttcaacgt cagagccagt tgcaaggttg cgtccgtaac aatgtttgca gacaccatgt
    48421 tttgtattac atgtgaagac agaacggatt gtcactgttt cgacacctgc atcaacgatt
    48481 tgttttgcga tgtcttcgga aatcaattgg tttcctgcaa tgatcaattc atttgtttca
    48541 ggatgacgta cttctttacg ggtgtaacgt cccactaaac gatcttctaa tgattcgatg
    48601 atttcatttc cctcgcggat ggcttggatt tcaagacctc ggtcagtacc gcaatcttct
    48661 tcacggatga ttacatcttg ggccacgtca accagacgac gagtcaagta acctgaatcg
    48721 gcagtcttca aggctgtatc ggtcatccct ttacgagccc cgtgagttga gataaacatt
    48781 tccaagacgg atagtccttc acggaagttc gaaatgattg gcaattccat gattcgacca
    48841 ttaggagcgg ccatcaaacc acgcattccg gcaagctgtg taaagttgga aatgttacca
    48901 cgagctccag aatcagacat catgaagatt ggatttttcg cttccaaacc ttccatcagt
    48961 ttttgttgga tgctgtcttt tgcagcattc caaacagcaa tgacacgttc ataacgttcg
    49021 tcatctgtga tcagaccacg acggaattgt tttgtaatag tttcaacttg tttatgggca
    49081 ttttcaatga tttcttgttt ttcattcagt acactgatgt cagcgatccc tactgtgata
    49141 ccggcatgtg tcgaatgttt gtaccctaag tctttcatac ggtcaagcat cttagaagtt
    49201 tcttttactt tgaatcgttt gaagacttct gcgatgatgt ttcctaagtt tttcttcttg
    49261 aacggtaata ctaattcttg ctctttgata tgtgcaggaa tatcagtacc agcttcaaca
    49321 aagtatttgt ccggtgtttg gacggtcaag ttgaagtctg ttggttcatt cagataaggg
    49381 aattcaggag gcatgatctc gttgaagatg atcttaccaa ctgttgtgat catcgtacgt
    49441 tctttttgcc attcagtaaa tggtttttca cctaatgagt tagggttcac accgatacgt
    49501 gaatgtaaat gaacatatcc gttgcgccat gcgataacgg cttcgttttg atctctgaag
    49561 accattcctt cgccttcgcg tccttcttct tccattgtca agtagtagtt acctagaacc
    49621 atatcttgag aaggagtaac aactggttta ccatctttag ggttcaagat gttttgagca
    49681 gctaacatca acatacgtgc ttctgcttgt gcttcttcat tcaatggaac gtggacagcc
    49741 atttggtctc catcgaaatc ggcattataa gcttcacata caagtggatg caaacggatt
    49801 gcgcgtcctt gtactaaaac tggttcgaat gcttggatac caagtcggtg aagcgtaggt
    49861 gcccggttca gcaatactgg gtgttccttg attacgtctt ctaaaacatc ccatacttcg
    49921 tcttcttgac gttcgatttt acgtttcgca tttttgatgt ttgaagctag ttcgcgttga
    49981 acaagctcac gcatcacgaa tggtttgaat aattcaatgg ccatttcttt tggaagtcca
    50041 cattggtaca tcttcaagaa aggtccaaca acgataacgg aacgacctga gtagtcgaca
    50101 cgtttaccta gcaagttttg acggaaacga ccttgtttcc ctttcaacat atgagaaaga
    50161 gatttcaatg ggcggttacc tggtccagta actggacgtc cgcgacgacc gttatcgatc
    50221 aatgcatcta ctgcttcttg cagcatacgt ttttcatttt gaacgatgat tccaggtgca
    50281 ttcaagtcta acaaacgttt caaacggtta ttacggttga ttacacgacg gtaaagatca
    50341 ttcaagtcac ttgttgcaaa acgtccacct tctaattgaa ccattgggcg taaatctggt
    50401 ggaataacag ggataacgtc cataaccatc cagcttggtt gatttcctga agcgcggaat
    50461 gcttctaaaa tatccaaacg gcggattgca cgtgtacgtt tttgtccttg tgcagatttc
    50521 aattcttctt tcaattcggc tgcttctgca tctagatcaa cattatcaag taattgttta
    50581 atcgcttctg cacccatagc agcttggaat tcttgtccat attgttcacg tttttcgcga
    50641 tattcgcgtt ctgtcaacaa ttgttttttc tctaaattcg tatttcctgg ttcgatgact
    50701 acatatgaag caaaataaat gatttcttcc aaagcgcgtg ggctcatgtc taatacaaga
    50761 cccatacgtg aaggaatccc tttaaagtac cagatatgtg aaacaggagc tgctaattca
    50821 atatgtccca tgcgttcacg gcgaactttc gaacgagtaa cttccactcc gcatcgatca
    50881 caaacgattc ctttataacg gatacgtttg tattttccgc aggcacattc ccagtctttt
    50941 gtaggaccaa aaatccgctc acagaacaac ccttcgcgtt caggttttaa ggtacgatag
    51001 ttaatcgtct caggtttttt tacttcaccg taagaccagc ttctgatttt ttcgggagaa
    51061 gccaaaccta tttgcatact ttcgaattta tttacatcga tcaaaagggg ttccctccat
    51121 ttcatttaat ggcaaaccgc aagacggctg atcttcgacc gccttgcagc tcatagaccg
    51181 gattagtcta aattatcttc tgcggtttca aagttttgga tgacatcgtc tttttcatct
    51241 tccacttgtt cagcagcttt cttttctaat tctttggcag tttgctgttc ggcaaatttt
    51301 gtcaaagcat caacagtgat caaatcgtcg tcttcatcgt ccatgtcgcg aagttcgatc
    51361 tctgtttctt cgatatccaa gacacgcatg tccagaccta gtgattgcaa ttctttcacc
    51421 aatacgcgga atgattccgg tacacctggt tttggaattg gttcaccctt gacgattgct
    51481 tcgtatgttt tcacacgtcc gactacgtca tctgatttgt atgtcaagat ttcttgtaaa
    51541 gtataagccg caccgtaagc ttccagtgcc catacttcca tttcaccaaa acgttgtcca
    51601 ccaaattgag ctttacctcc caatggttgt tgtgtaacca atgagtaagg tccgattgaa
    51661 cgtgcatgca atttgtcatc gaccatgtga gcaagtttga tcatgtacat cacaccgaca
    51721 gacacacgtc catcaaatgg ttcacctgta cgtccgtcgt acagaacagt tttcgcatcg
    51781 ctggccatac cggcttcacg aactgtttcc catacgtctt catcacttgc accatcaaat
    51841 actggtgttg ctacgtggat acctaactga cgtgcagcca tacccaagtg taattccaat
    51901 acttgcccga tgttcatacg tgaaggcacc cctaaaggat tcagcatgat atcgattggt
    51961 gttccatcag gtaggaatgg catatcttct tccggcataa tacgggatac aaccccttta
    52021 tttccgtgac gaccggccat tttatcccct tcatggattt tacgtttttg aacgatataa
    52081 acacgaacta acatgtttac gcctggtgac aattcatctc cagcttcacg agtaaagatt
    52141 ttcacatcgt gaacgatacc gccgccgcca tgtggtacac gtaatgatgt atcgcggact
    52201 tcacgagctt tttcgccaaa gatcgcatgc aatagacgtt cttctgcaga taattctgtt
    52261 accccttttg gcgtgacttt ccctacaagc aaatcgccat cttggacttc tgcaccaata
    52321 cggatgatac ccatttcgtc taaatctttt aatgcgtctt ccccgacatt agggatttcg
    52381 cgtgtgattt cttcaggtcc taattttgta tcgcgggctt ctgattcata ttcttcaata
    52441 tggatagaag tgtatacgtc atcttttacc aaacgacggc tcatgatgat cgcatcttca
    52501 tagttgtacc cttcccaagt catgaaacca actaaaacgt tttggcctag agccatttcg
    52561 ccttgttcca tagatggtcc gtctgccaga gtatcccctt tttcgacttt ctcacccaaa
    52621 tgaacgattg gacgttggtt gtagcttgtt ccggagtttg aacgacggaa ttttgttaca
    52681 tcgtatttat ctaatgcacc gttgtcgcgg cgtacgcgga tttcagaagc atcaacgtat
    52741 tcaacgacac catcatgttt acataacaaa gcagctcctg agtcatgcgc tgatttgtat
    52801 tccataccag taccaaccca tggtgcttga ggattgatca acggcactgc ttgacgctgc
    52861 atgttggcac ccatcaaggc acggttggag tcatcgtttt ccaagaaagg aatacatgct
    52921 gtcgcaacgg caactacttg tttaggtgat acgtccatgt agtctacttt atcaatagat
    52981 acttctaagt tttcactttg agcacgagcc ataacaacgt cttgagcaaa tgttccatct
    53041 tcattcaatg ggctgtttgc ttgggctacg atgtaatggt cttcgatatc agctgtcaag
    53101 tagtcgattt ggtcagtaac acgacctgtt tcacggtcta cacgacgata aggtgtttcg
    53161 ataaatccga atttatttac tttcgcataa ctggacaaac tattgatcaa cccgatgttt
    53221 gggccttcag gagtttcgat cggacacata cggccatagt gggagtagtg aacgtcacga
    53281 acttcataac cggcgcggtc acgagtcaat ccgccaggtc ctaaggcgaa aagacgacgt
    53341 ttgtgtgtca attcgccaag agggtttgtt tgatccatga actgtgacaa ctgagaagaa
    53401 ccaaagaatt ctttgatact tgccacaact ggacggatat tgatcaattg ttgaggtgtc
    53461 aatgtttcag tatcttggat cgacattctt tcacgaacca cacgttccat acgtgctagc
    53521 ccgatacgga attggttttg taaaagttca ccgactgaac ggatacgacg atttcctaag
    53581 tggtcgatat catcgacatt gccgatacct tccatcaagt tcaagaaata gctcattgaa
    53641 gcaatgatgt cggctggacg aaccgttttt acagctgctt ctggatagcc gtttccgatc
    53701 acgtttactt cacgttcagg gtctcttggt gaaaatactt tgatgacttg gacagtcatt
    53761 ggatctgtga ccacaccgtc ttctgatgga tagtaagtca ctgaatttag gccattatca
    53821 atgaaaggag ctagtgtttc catgacttgg tgagtcagaa ctgttccttt ttcaacgatg
    53881 atctcgcctg tttctggatc aactaatgtt tcagctaatg tcagattcaa tagacgtgtt
    53941 ttcaaatcta attttttgtt tactttgtaa cgaccaacgt ttgctaagtc gtaacgtttt
    54001 ggatcgaaga aacgagcatt cagcaagtta cgtgagctgt ctgctgtttt tggttcgcct
    54061 ggacgcaaac gttcgtaaac gtctttcaaa ccttcttctg ttcttgagtc gcttgcgttt
    54121 ttatgcaagt ctttttcgac tgtattgcgt aatgtttcgc tgtcgccgaa gatttcaaag
    54181 atcgtatcat cggaaccaaa tcctaacgca cgaactaata cggttaaagg aatcttacgt
    54241 gtacggtcga tacgtacata agagatgtct tttgcatcag tttccatttc tagccaagct
    54301 ccacggttag ggataacagt agaaccgaat ccttcttttc cgtttttgtc gacttttcca
    54361 tggaaataga cacctggtga acggactaac tgagaaacga ttactcgctc tgccccgttg
    54421 atgatgaatg ttccttgttc tgtcattaat gggaaatcac cgaagaatac ttcttgtgct
    54481 ttgatttcgc cagtctcgcg gtttgtcaaa cgcaatgtta catgtaatgg tgcagagtag
    54541 tttgcatcat gcgcacgagc ttctgctact gtatactttg gttctttcaa ttcataatct
    54601 acaaattcca aagatagatt gccattgaag tcatcgattg gcaaaatgtc ttcaaacatt
    54661 tctctaagtc cctcatctaa gaaccattgg taagagtctg tttgaatttc gatcaaattt
    54721 ggtaattcca acacttcact gattcttgcg aaacttctac gttcgcgatg ttttccgtat
    54781 tttactacgt gtccagccaa gctcttcacc cctctaaaat gtcattcaaa aagactaccc
    54841 gatcaattgt tacaataata tttcaaatat tatttttagg taacaacaga cattttttgc
    54901 ggcttttagg caaaaaaaaa ccaaaaatag aacattctct tgaaacctta cttcgtgagc
    54961 ttctactttt gtttccttat attaaagccg aaacggacaa tatttcgttt ctgccttttc
    55021 ttcatcagat agtttttgca acttcatata ctacacttat tttctataaa agtcaacact
    55081 tcctggcgtt tcttctgctt tttcgcaaca ttttcttaat gttttcaatt ttatgttttc
    55141 aattatctga caattcttaa ttgacagacc tttttatctc ggttatactt tagaaaattc
    55201 attttgtgga ggaacttcta tgcgaaccaa catcaaccaa atcgcaccaa cacaactgaa
    55261 taacttttac tttagctact ttagataggt ttgtattcat catgatggat acaaacgtta
    55321 aacgacagtt tgtatctatg atggctaaag aactttgtat tgcagcaaaa agccacgtag
    55381 acaaaatgct aagtggctga gcagtgaaaa tacaaagtaa aagtatcaag cattctactt
    55441 tttcaattta ttttccgact tttcaatcca tttggacatt ttgtctgaat ggattttttg
    55501 tttcccaaaa ttaaggagga aaaagaaatg aaaaaaaata taatcatcgg attaggcgct
    55561 gccctgctac tagctttagg aggttgcgca caaaaagaca attccgcaga ggcatcagat
    55621 ggacaaaaaa caattggcgt attgcaactt gtacaacata actcgttaga tgctgcctat
    55681 aaaggattca aagaaggatt agcagaagaa gggtataaag aaggcgataa tctaacaatc
    55741 gattatcaaa atgcccaaaa caatcaagac aacctgaaaa gtatgagtga gaaactcgtc
    55801 aaagaagatc ctgatttgct acttggaatc gccactccag cagcacaatc tttattaaat
    55861 gaaacaacca ctatcccgat gactgtcaca gcggtcacag acttggaaca agcccattta
    55921 gtcacatcca atgaaaaacc tggcggcaat gtgactggca cgacagatat ggtgccaatc
    55981 gaaaaacaaa tcggcttact gttaaatatc gtgccagacg ccaaaacaat cggcatcatg
    56041 tataacaacg gtgaagcaaa ctctaagatc caagctgatt tggcagaaaa agaattgaaa
    56101 aaagcgggcg tcaaagtcaa agtactgaca gcaaattcca caaatgatgt ccaacaagtg
    56161 acgaaaagtc tagctaaaga tgtagatgga atctatattc ctactgataa cacgttcgca
    56221 agtgcggcaa gtgttgtcgg tgaagtcgca aaagaaacaa aaacacctgt cgttgctggc
    56281 gcagttgatc aagtcaaaga cggcggtcta gcaacagtag gaatcgacta cgaaagcttg
    56341 ggcaaacaaa cagggatcat ggcagcaaaa atcttagacg gcaagaatcc aagtgaactt
    56401 cctgtcgaaa cagcgaatga tttggaactg tatgtcaata aagacatggc caaagcatta
    56461 ggcatcaatc cagacagcat caaagaagct gaataaaaca caggaggatt ctcatgacaa
    56521 atttgctctc tcttttctta tcatctgcat cgcaaggggt cttatgggcc ctacttgcga
    56581 ttggtgtata cgtcactttc cgtatcttgg acattgccga cttgactgct gaaggaagct
    56641 ttcctttagg tgcagggatc gcggcagtaa gtattacaaa tggctatcat ccattgacag
    56701 cttgtcttct aggcttttta gggagtgcgg cagcagggct tgtttccgga ctgcttcaca
    56761 caaaattgaa aattcccgcc cttcttgcag ggatcatcac catgactggt ttatattctg
    56821 tcacatcccg tgtgatgggt gcgccgaaca tctctttatt gggtcagcca acgatctttc
    56881 attgggtcga gtcattcggc ataacaaaag acaacgctgt actgatcgtc ggtctgcttt
    56941 ttgctttgat cgttgtatgt ttacttgttt tgttcttacg tacagaaaca ggacttgcta
    57001 ttcgtgcaac tggcgataat ctggcaatga gcgaagcaaa cgggatcaac acagataata
    57061 tgaagatcat cggttatatg atttctaatg gatgcattgc tctttctggc tccttgcttg
    57121 cgcaaaacaa cggctttgct gatttaaatt ctggtattgg aacgatcgta attggacttg
    57181 cttctattat catcgcagaa gttttgttac gtaaccagcc actatttctc cgtctcttga
    57241 ctgtcgtttt aggtgcggtc atttatcgtt ttattttagc gttagtcttc gaactgaatg
    57301 ttgaaccaag tgattcgaaa cttgcttctg ctttcgtgct agtgatctgt ctttctttcc
    57361 cgcagatcca gcaaaaattc catttacctc aaaaaaagaa aggcggtagt gtcaaatgaa
    57421 tgacgtctta acattggaaa atctccatca tacctttgaa aaaggaacga tcaacgaaaa
    57481 tcatgtcctg aaaggcatta atctttcctt agatcccggc gacttcgtga cgatcatcgg
    57541 tggaaatggg gctggtaaat ctactttgct gaacagcatc gccggtacat tttctgtcga
    57601 tcagggtcaa attttattga atggaaaaaa tatcacgaaa aaatctgttg tcgaacgttc
    57661 gaaatccatc agtcgcgtat tccaagatcc taaactaggg acagccgttc gtttaacggt
    57721 agaagaaaat cttgctcttg ctatgaaacg aggaaaaaaa cgcggcttct tccgtggtgt
    57781 caaaccacaa gaccgaacat ttttcaaaga ccagctggtt cggctaaatt tggggttgga
    57841 aaatcgttta tcctcagaaa tcggtttgct gtctggagga caacgtcaag ctattacgtt
    57901 attgatggcc acattacagc gtccagaact gatcttatta gatgaacaca ctgcagcgct
    57961 tgaccctgct acttccaaaa cggtgatgga attgacagaa aagctgatca ctgagcagaa
    58021 tctcactgct tttatggtca ctcatgacat ggaagacgcg atccgctatg ggagtcgact
    58081 gatcatgctg catcaaggaa ggatcgtcgt agatatccag caagaggaaa aacagcagct
    58141 cacagtgccc caattattag cgttgttcaa acaaaacagc ggcacggcat taaaagacga
    58201 tcaagtactt ttatcttcat aatcagaaag aggatgggac aaaaaatgaa cgcccaccct
    58261 cttctttttt ctctttcctt ctttcgtttt tgctttatgt atcaaccgtt gtttcttcga
    58321 aaactatttt gttctctcct tcttcctgat agaatggatg ggaaaggagc ttgatgccat
    58381 gacaacgaaa gacaaggtac ttcaattatt gaaaaagaca ccagattttc tttccggaga
    58441 aaagttggca cagcaactgg aagtttcccg gacaagtatt tggaaagcca tcaaagaatt
    58501 ggaaaaggca ggctatcgtt ttgagcacgg gcctacaggg tatcgttacc tgccctctga
    58561 cgtactagat gcaaaagaaa tccagcaaga gctacggtcg tttattcctg atttgaaagt
    58621 acaaacgatg atgacttctg aatctacgat gaaagatgcc aaactggcag cagctcaagg
    58681 agattctgtt tcgaagttat ttgttgctga tacacaagaa caagctcgtg gacgttttgg
    58741 acggcctttt ttcgctgaac ctggacgagg aatctacatg agtctcttgc tccatccaga
    58801 caaacggttt gatgaattgc ctcaatatac gatcatcgca gccagtgcct gtgtgaaagc
    58861 cattgaatct gtaaccggtc gttctgcttc gatcaaatgg gtcaatgata tctacatgga
    58921 tggaaaaaaa gtctgtggga ttttatctga agcagtcagt gatatggaaa gcggcaggat
    58981 cagtcatatc atcattggga tcgggcttaa tttttctgtt cctcaaaatc attttccagc
    59041 tgatctccaa gaaaaagcca tctctgtctt tcccgacgga caagctagtg tcacaaggaa
    59101 tcagctaatc agcaaaatct ggcaagaatt gttcacttat ctttctcacc cagaaaaagc
    59161 gttggacttc tataaagaaa aatcattcgt attgggaaaa accgtgtcgt tcaatcaaaa
    59221 aggaacgact tatgtcggtc ttgcaaaaga aatcggtgat catggcgaac tgatcgtcca
    59281 aacaaatgaa cagaagatga gtttgtcttc tggcgaaatc agtctcacat ctatccgtac
    59341 gcaataatta tcagatttca caaaaggaca cctgatctca tacagtgtcc ttttttctga
    59401 attctcttca aaagactctc cttattctat cagctgacag tttctctatc ctctagaaaa
    59461 aggtattcta aaaatatcta taaagcatgg tacactttct ttaaatgaat caatgcaaaa
    59521 aacaagagag tcatcagttt acacgatcaa aagagatagc aggccgcttc ctttttcaat
    59581 cttatagata gaccatgggt aacgataaaa acaaaaaaga acttgcgact tattttgccg
    59641 taagttcttt tttagctagt gccttaatct cttaatccca acaaacgtct cgcatagatc
    59701 acctgaccag agtgcatagc agcatcatca ataatagaaa cgagacgcat tcctcgtgtg
    59761 actggtggtg tccagttctc gtcaattact tcatctagct cggaaggttc cagccttctt
    59821 aagtaagaaa tagcaaaatc agcagctcct cgcaagtatc ctagcagatc ttctatttga
    59881 ttagcttgga tctgctttgc ttgttccaga ctatgcttcc aatcttgttc gttatctgct
    59941 atcgtcatcg gaaaccggct ttcccagtct tgactatgcc aaattggaga agttcctgct
    60001 aaatcagcaa tctggaaatc taattctcga gcggtatgcc aagccagcca agtcattgat
    60061 ttgatctgcg gggctctatc ttccactgga aaacgattgg cctcctcaat agagacgcct
    60121 tcaaacaaac gttcaaaccg atcgtatgcc cgatccacac tctcgcatag catatctaaa
    60181 taatccattt attttcctcc tttttattta tcctacttac tttttcctaa ttctaggata
    60241 cctacttata tgttactaca cacccttatt ttttacactt taacagtcac catcatcaat
    60301 agtttggaga ttgaaagaaa aagatagtgt cgaactatca attgaacaag aagcaacaat
    60361 atgaacgttt cattaatatt ctttaaataa acaatgattt ttcaaacgaa cagtttaaga
    60421 ctaggagaaa aagagtataa taggaaaatg tttgaaagga aaggaagggg aaaaatgaaa
    60481 aaaagggcaa gtttactagt cctagttgct ttgttgttca atatcttttc gtatggtgtg
    60541 gccgtttcag cggaaacata cattcctaaa gaccctggga atatttataa tgatctaact
    60601 ggtaactatg gcttgttagg aattgctagt caattccacg tatttgctaa aaataagaca
    60661 actgtgaatg cacatacaga cggaaatata gcaacacatg aacttgatgc aaaaaataat
    60721 tttggtacag agcttcatga aggtttattg aaccaagaaa taaattatat acaaaaaact
    60781 gattctttcg ctaattcttc aggcatacct acaggtagca caagaatgaa taaatttgtc
    60841 gttggcagta acgtggatgt agcacttcaa gatggtcatc ctacagtcaa tggatccagg
    60901 cttgatcact taactccaga tgatttttat aaagatcgtg ctgggcatga atatattgat
    60961 ttcgagcagg aatttgctaa attgaatgta gcagcaaatg atcttgcaac gatcactcca
    61021 gctaaaacat atactgctgc agattttcca gacatgaaca atcggacaat cgatttaact
    61081 gatttatcag ataccgggtt tttattggta aatatcgatg cagaagtgtt aacgatgaat
    61141 acgccacttc aaatcattaa tccaaatgat caagtagttg tatttaatgt catcaacagt
    61201 tcttctgcat tgaacgtaca gtcaccaatc aaatacaatg atcgttcaaa tcatgaaaca
    61261 gaagattttt cggatgcgaa tatctcttgg aattttggaa atgagatgac tgatttgaca
    61321 atcagtgctc cttttcaagg aacaatcctt gcgcctaatg ctactatccg cattactcaa
    61381 aaccaagacg gaacgattat aggaacaaat gtgattttag atgcagctac caatcgttgg
    61441 gatcctaacg aaatatttat cagcgacaat ggtacagata cgacagacac aagcgacagt
    61501 acagatacat ctaccacaag tgatagctca gattcatcaa cttcaagcga cagtacagat
    61561 actacctcta ccacaagtga tagcacggat acatcagctt caagcgacag tacagatact
    61621 acctctacca caagtgatag cacggacaca tcagcttcaa gtgacagtac agatactacc
    61681 tctaccacaa gtgatagcac ggacacgtca gcttcaagtg acagtacaga tactacctct
    61741 accacaagtg atagctcaga ttcatctacc actacaagtg atagcacgga cacgtcagct
    61801 tcaagtgaca gtacagatac atcctctacc acaagtgata gctcagattc atcaacttca
    61861 agcgatagta cagacacgtc gtctaccaca agcgatacac gtcatgcaag tgtctccttc
    61921 tcaaaaacaa gttcggataa caggacgaac tttaacttaa ctaataaaac aagcaatgac
    61981 aatggaaaca aatcaaaaag agcacttcct aaaactggaa gtcaatcgaa taattggata
    62041 acactcgcag gcgttatatt acttgtaatc ggtttaagaa tcacttttat gagttataag
    62101 aaatcgaaaa ggtaaaaaaa tattcaagat aaaaaagagc aacgtcgcta acggacgttg
    62161 ctctttattt ttgcttttta tcataaatac aagaactaca attcaatttg tcaaaaaaca
    62221 cgtagatttc cttctgagaa atttatttac gtatcgaacc tttccctatt cagtctttgc
    62281 gatccaaaat cagttgggta agggaaaaga gattctcttt tgttcgaaga gaggtggcag
    62341 gtaatttctt tatttccttc aaagcctttt ctgtgtaaga ggccgctaac ttcctagctt
    62401 cttccacgcc tcctgtatgt tcaacgattt tgcgtacggc gtcgacatct tcttctgtca
    62461 ttgccgcttt cttctctaaa taaggtagta gctttggttt gtttttttgc aaagagtaga
    62521 tcaacggtaa agaatagacc ccttgacgca tgtcttctag tacaggtttg ccgatttcct
    62581 ggctctcttt tgtataatct aaaatatcat cgataatctg aaaggccatc ccgatagaaa
    62641 gtccgatatc tcctgctttt tttgcgaagc gctcgcttgt tccactttca aaagcgccaa
    62701 cagaacaact taattggaac agttcagctg tcttacctga aatattttcc aaatattctt
    62761 ttacagaaag ttcataatta tagcgatcat ccatctgccc taattcgcca ttcaatactt
    62821 tttccatact gcgagaattc aattggagac ttttcaagga agtggagtaa tcagacaata
    62881 gtttaaaaca gcagacaaat aaatagtctc ctgcatagac agaaacgaca ttaccaaact
    62941 gactacggat attaggctga ccacgtcgca gatccgcttc atcaacgata tcatcatgga
    63001 tcagtgtggc cgtatggagc atttcgatag aagctgccaa agcgatggct ttgtctcgat
    63061 cttgctctgg tccaaattgt gagaacaata gctgataggc aggacgcaac agctttccgc
    63121 cggcatggat cgtttctttg attgcttgtt cgacaggttt gtttttcagg cggacagttt
    63181 cttccatccg ttttagtgtt tctgtcaatt ctttacttaa agcaggatag ttttcccaca
    63241 ttggatgtag cttcataaaa aaactccttg tgtgacgatt attttttggt aaacattggg
    63301 ctccattttt gtttggttcc ctgctcataa tctttttgga atcgttgaag tgtctgcaca
    63361 tgtcgtaaaa gataattcgc tgcgatcccg attgcaatcc ctgataaaat ccctaaaact
    63421 gaaaggactg gcaagtacaa catgactgtc catgattgtg caatgaaaga agccatcaaa
    63481 agctgtccga cattatgcat gaaaccgccg gccgcactaa ttccgatgat actgacacgt
    63541 tttggtccta attgcttgac caataacatt ccaaaataac taagcaaagc accactcata
    63601 ctataaagga atgtggaaat cgttccgcct agcaaagtag tcaggaatag tcgtagccaa
    63661 agtagtaaga agctgtcttg ttttttcatg gtaaaaatgg caacgatcgt gattagattg
    63721 gctaagccta gttttgcacc aggcgcaaaa gaaaaaggat aagggatcat attttccaat
    63781 aaaccaatga tcactccttg tgcaacaagt agcgaaatat aaatcatttt ttgtaattta
    63841 ctcatggtgt gtaaaggtct aataaatcag actgtcttcg tcacctccat ctgttgaacg
    63901 gacttcgatc actaatttat gtggcaaaca gacgatggtt tcgccatttt tcgttgccca
    63961 gcctcgacgt acgcagatct ggtcatcgca atctgcttct tttatccgta ctcgatcgcc
    64021 atctatctcg atcaagttat aatctccgtg ttcatctttg taagtatatg tgttgctctc
    64081 ttctccagcc atcaaaggga atgttttgat ttctgtgcca tccacacgca gtaccgcttc
    64141 tttttctgct gttgcggtgt tttgctgaag accaaaaatc atgactggta aaaaagaaag
    64201 aacagtcaaa agcaaaataa taaaaacatc ccaaggtttg agcagacttt tttcagcaaa
    64261 ttctttaagg ctcatttttg ttctcctttt tccagattaa ctcacccaat tttagcataa
    64321 aaacaagagg ttgtgacacc tgtgtcttgc tttgagtaat aagaacgaag aatagaaaac
    64381 agctttccat gttttctaat tctttgggct tattaccgag aagcagcagg tggaacaccg
    64441 tttataacaa gaggttgtga cagaagtggg caacttcaag aaataagaag gaatttacga
    64501 aaattacttt tcaagttttt gtgaaatttc ggcttatttt tcgaagaagc tacttctgga
    64561 acaccgttta taacaagagg ttgtaacaga ggttgtgaca cctgtgtctt gctcatggag
    64621 gcttatctcg ggcatcttat tcctttaatt ttacataaaa aggaggctgt gacggtttgt
    64681 ctcagcccct ttctattgaa atcttgcatt caaataacgt tctttatcta tttatttctt
    64741 gccgtactgg gaacttggtc taccgtacca ccaattacct agtgtacgtt gtagccatgg
    64801 aattacccag cgatccaacc caatcgcacg tccggaaccg ttcatcaatg cgaaagcaac
    64861 aaaaatgaac caaatgttta cccagtaaaa catgcctgat aagcagaacg tgatcaccaa
    64921 tacaatcgtt gtcgcactgc atagccatgt gaataatcca gcaatcaatg ctaatgccaa
    64981 ggcaatttca aagaatacca tgaatttttg gaagaacatt gctacatcat tgtttggcat
    65041 gatcacttgc atgatctttg tgaaccagtc tggtgacttc tcaatcacag ccattggttc
    65101 ttcaccgtat gcataactca aaccaaaatg tgcagcttga gtcgctgctt ctccggcact
    65161 tgcccctgct ccgctggcag ctgtggttgc atcaccagca gcagaagcac cactggcaac
    65221 atctgttaca ttgtattccc atgtccaagg gaacacactt tttccagtga accatgatcc
    65281 ttcaccaaac caagtacttg gtttgaacac ttcaccattc ccaatgattt ttttggaagc
    65341 ttcaacgaac caaactaaac catagaaaat acgaagtggc acactccata agacattgcc
    65401 gtaacgagaa gaatgtcctc gagtcacgtt gcgatcatct ttgatatgga aaaactcatg
    65461 cattaaatat tggaacatat aataaccaga acgaatatca aagaagtatt tcaaattcac
    65521 gatatgtttc attacgatag ctaaaaatcc gcttaaatgg atcttatcaa ataggttagc
    65581 taccccccat ttggcaccaa tagaaaccat aaatccttga tagtttcctt taaatttatg
    65641 cttttctccg cctttgatcg aagccacgat attcgctgcg gctgtgtgtc ctgtttgttc
    65701 agcagcttga acgatctgcg gtgtcggtgt tcctgggaat tcttcataaa agactaaatc
    65761 accaatgata tagatgtttt tatcttcgta gccttttgcc tgcatatatt cattggcgat
    65821 caaccgctgt ccgcgtgctg attctaatcc aaagtcagca gcgtcagaag ttcctttgac
    65881 accagctgtc caaatcagtg tgtgagtcgg aatgttgcgt ccgtctttca atttgatatg
    65941 atcagcagcc acttctacga ttggtgcatt caataataat tccacgttgt gcttacgcaa
    66001 atagcgttca gcttttgcag catcatttcg atccaacata ttcaagatcg tcggcattgc
    66061 ctctacgacc atcagtgtga tttcgtttgg atctaattta taatctttgg ctaggcgatt
    66121 tttccagtcg atcagttccc caatcatttc aataccggta aatccagaac cacagacaac
    66181 gaaagtcagc atggctttac gcagttcatc atctggttcc aatgcagctt ttgctactgt
    66241 cttctcaata tgttcccgaa tccttacaga atcttcaaat gaccaaaggg tgaatccgtg
    66301 tgctttgaca ccaggtgtac caaagtcatt cggctctccg cccattccaa ggatcagctg
    66361 atcaaaagga taggaaccta atttggtctt gacgattttt cgttctttat cgataccgat
    66421 tacagtgtct gtgacaagtt gtacattctt ttgacgagca aacaaatgct gcagatcgta
    66481 ttggattgct tctggttcta cacgtccgcc agctacttca tgcaactcgg tcatcatagt
    66541 atggtacgaa tgacgatcga tcagagtaat tgctacatct tcttttttca gtttctttgc
    66601 caagtatttt gtagctgaaa caccggcata tcctgctcct acaacgacaa tattttgctt
    66661 tgccattgat catccgctcc ttatagacta tctatataat gaatatatga taattctttt
    66721 agtttgatac ttattacaca atctccatat attatataga tgttctgttc ttttgtctaa
    66781 ctccttttgg atttttttca aattttaaaa taaagcgttt tttcacgaaa tttgctgaaa
    66841 gaaaacgttt gaaatattac atgggttatt tatcaaaaat agttgactta taggctaaaa
    66901 agattagaat gtataatgaa tcacaaagaa aaattgtgga ctagaaatgg aatggtgggg
    66961 tattaaaatg aagaaaaaac gttttgtaac tggatttgcc gtattagcat tttcagctct
    67021 agtgttaggc gcttgcggcg cagatgacaa cggaagctca aactcttctt ctgaatcatc
    67081 tacagcacaa tcttcgactg caaaaagcac aacagaatca tctgcaaaag ttgtagctgg
    67141 cggagatttg caagacggca catataaatt agaagaaaaa aattattcaa atggttaccg
    67201 tgcagtcttt gaaatggttg taaaagacgg caaaatcact gaatcaaaat atgataacgt
    67261 caatgaaaac ggcgaatcaa aaacaaaaga tgcagaatac aacaaaaaca tggaagcaaa
    67321 atctggcaca aacccagaaa aatacattcc tgagttgaat gaccaattct taaaagcaca
    67381 aagcgcaagc ggtgtggaag tcgtaactgg agctactcac tcatctgaaa gcttccaaaa
    67441 ctacgcacaa caattgatcc aagctgctca agctggtaac acagatacaa tcgaaatcga
    67501 caatggtgct gatttgaaag acggcactta caagcttgaa gaaaagaact actctaacgg
    67561 ctaccgtgtg caattcgaaa tgacagttgc tggcggcaaa gtaactgaat caaactttga
    67621 ttatatcgac aaagacggta aatcaaaaca agatgataca gaatataatg aaaatatgaa
    67681 agcaaaaagc ggtacagaac caaaaactta catccctact ctgaacgacg aattcgtcaa
    67741 agcaatgggc gaagaagatg gctcacctgc taatgtagaa gttgttacag gtgctacaca
    67801 cagctcacat tcattcatca tgtacgcaca acaattagtg aatgcagctg aaaaaggcga
    67861 cactcaaaca atcgaagtag ataatatcgt aacagaaaaa taatcacata atcatactaa
    67921 taaaagttgg agggtgggac gatagccaga ttaggcagtc gtctcacctt tcaaaatgaa
    67981 gagaggaaaa agatgcggaa aaaactacta ttttccttaa tcacactggg catctctttt
    68041 atggtgctaa ctggctgcag caactcagca aataaaacaa ccactgaaag ttcagcagct
    68101 accaagatat tgaaagatcc atattctgat gaacagttct tgttagggac ttacgttcgt
    68161 atccgtgtgt atgatgaagg caaagaatca gcattgaaac ctgccttcga tcgagtaaaa
    68221 gaacttggtg ataaaatcac gatcaatcaa aaaggttctg agatcgatga agtgaatgag
    68281 caagctggga tcaaaccagt aaaagtttct gatgacgtct acacgttagt caagcgggcc
    68341 tacgaatata gccaagattc tcaaggcgga ttcgatatgg cgattggtgc aatcacacaa
    68401 ttatggcgaa tcggttttga tgacgccaga aaaccttccc aagaagaaat cgatcaggca
    68461 ttgaaattag tggattatca taagatcgaa ctaaatgaca aagaaaaaac tgtttatcta
    68521 aaagaaaaag gaatgatcat cgatttaggt gcaatcgcca aaggttacat tacggacgaa
    68581 gtagtgaaag tcttgaaaaa acaaggagtg accactgcca tcgttgactt gggcggtaat
    68641 gtctatgtct taggccacag cccaagaggc gaaaaccaag actggacagt cggcatccaa
    68701 gatccgaaca tggctcgcgg atctgtttta ggttctatta aagaacggaa caaaacatta
    68761 gttacctctg gaatttacga gcgttattta gaagtagatg gcaaaaaata ccatcatttg
    68821 tttgatccaa aaacgggtta tccgttcgac aatgatatcg caagtgtcac gatcataaca
    68881 gataaatcaa ttgatggtga cgggttgtct acagcagtct tttcaatggg tgtgaaaaaa
    68941 ggactggaat acgtcgaaag cgaattgaat aacgggacgg aagcgatctt cgtgacaaaa
    69001 gacgataaag tatatgtgac cgatccaatc aaagacacct tcaaattatc ggaggactcc
    69061 cattacacga tgggtgaccg ttctgaacta aaataaggag tacgaaccat gacactaaaa
    69121 atctttttag aagtagttga aatacgaaca aaggtggcta gtgtatttcc ttttgcggta
    69181 ggggtattat tctctatcgc tttttttcat gagatacatt ggctaaacac ttttttattt
    69241 tttattggaa tgctagtttt tgatttagcg actaccgcta tcaataatta catggattat
    69301 gaaaaggctc attctgaagt atataagtat gaagaaaatg tcattggccg cagaaatcta
    69361 tctcctgtat tgattcgaaa catgatttta gctatgattg catttgtttt tgttatcggc
    69421 gttattctaa cgatcaatac gggttggctt ttacttttga tgggatttat ctgttgtttt
    69481 attggagtat tctatacatt cggaccgatt cctttatctc gaatgccttt aggggaaatt
    69541 ttcagtgggg tgactatggg ccttggaatt tttgccatgg tcgtttatgt caatacattc
    69601 ggttcagatg tctatgatct gacagtcaac ttttcatctg gacaatttgc tttggtagga
    69661 aatctttggg ctatagcagc aatcgtatta gcttccttac ccttggtgtt tacgattgcc
    69721 aatatcatgt tggctaataa tttgcgggat cttgaccgtg atattgaaaa tcatcgctat
    69781 acactagttt tttatatcgg acgtcctgcg ggaacagtct tattccagct attgatgtat
    69841 gcttgctatt tcgtgatttt aatggggttg ttgagcggta tctatgaatg gccgatcttg
    69901 gtcaccttct tgaccttgcc aattgtgaag aagcatttgg atcaattcaa gcaaacgtta
    69961 ccccagccta tcagtttcag ctattccatc aaaaatatgg tcttattcaa tgccagttat
    70021 gtattggggc tattactaac aatcattatg caagccattt aacagcatga agaggttgtg
    70081 aaaaaactgc cctgcatcaa gtaataagaa cagccaaata aaaatagctc cttatatttt
    70141 tcgtttggtt gggcttatta ccgcagatgc aagcttttga acaccgttta tcaggaggtt
    70201 gtgaaaaagt cgcttagttt cgagtattaa gaaaaatttt gtgaaaatgg ctttccacat
    70261 ttttgcataa atttggctta ataccgagaa actggctttt gaacaccgtt tatcagaggt
    70321 tgtaaaatca gtgttttgtc ctgagtatta gagaaacaaa tggaaaaata gttcctcata
    70381 ttttgccgtt ttttgagcta ataccgaagg acagctgatt gaacactatt tgttcggaaa
    70441 aagtgactaa ctatgacaca atttttttca cagtcccttt tttcacttga tttgattcgt
    70501 accagcgaac ttgacgagaa ctttcttggt ttggtaacta tatggtattc ttttaacatc
    70561 ctcgtttgct tcttctaatt tatttataca aagtcacact tttttaaaca tctgttcttc
    70621 gatacatttc tatttctttt aaacatcgat accaatattt tgaacttaag agtgcatacc
    70681 aaatggattc atttactctt cgtcagttac gaagataatc aaaatctcca acttccctac
    70741 ttatttcgtt ttgagaatta tcgaagcaat cttacataaa taactaaaaa acaaaaaacc
    70801 agaaaatcag tatactgatt ttctggtttt acatggtttg ttcaataaaa agctgttgga
    70861 actgttaata ttaaattatc gcatcgttac gaattcttcc gctcctgttg gatggatggc
    70921 tactgtattg tcaaagtctt ctttagttgc acccatcttg atagccacgg caaaaccttg
    70981 cagcatctca tcgacaccaa caccaatagc atgtaagccg acgattcttt cttcttttcc
    71041 gacacagatc aatttcattt cgcatttttg gcgataatca tttaatgcaa agtacattgg
    71101 ggtaaagcgt gaacgataaa ctttcaattc attttcgcca tatttttcca atgcttcttc
    71161 ttctgtcaaa ccaattgttg caattggtgg atgcgtaaag acgactgtcg gaacaagttt
    71221 atagtcaaga taagaatctt tctttccatt aaataaacgt tctgataaac gtctaccagc
    71281 tgcgattgca acaggagtca gatctaattt accgatcaca tcaccaacag caaaaatatg
    71341 ttcatccgtt gtattttggt atttatctac tttgataaac ccttttttgt ccaattccac
    71401 atttgtattt tccagaccta atttttcggt attcggtaca cgtccaccgg caaaaagtac
    71461 acaatcacca gttaaggttt caccattttc aaatagcacc gtatattcgt cattgttttt
    71521 agtaatttct ttagcaatat gatgtgcgta agtttttatt ccagcttctt ggtacatttg
    71581 gatcaaattg tccgaaagca ttttatcaaa cgttctaagt ggtcgttctt ttcgatatgc
    71641 ccacatgact tcactaccta aaccacgaaa tacacctgaa agttcagccg caatgtatcc
    71701 agcacctaaa acgattgctc tcttaggtaa ctctgttaat tcaaaaaatc catttgaatc
    71761 aattgcgtat tctccaccag gaatggtcat tttacttgca cggcctccag tagcgatcaa
    71821 gattttagga gcacgatagg tttcaccgtt tacttcgatc gtttgactat cgataaaagt
    71881 agcgtaacct tttatagcct cgacatgatt gctatctaat cctctttgat aagcaccgtg
    71941 tagaaaatca atatattttt ctcgtctttc tactagaccg gcgaaatcaa cattttttaa
    72001 atctgcttgg ataccgtaac ttgaggcatc tcgttcaatt gtttccaaaa tcgtacttgc
    72061 ttgccacata acttttttag gtacacaacc gacgttgaca catgttccgc ccaattcatt
    72121 cgcttcaatc agcaaaactt ttgcgccatg catccctgct ctgtttgctg aagcaatacc
    72181 gccagagccg ccaccaatta cgatgtaatc atatatttgc attttgttcc tccttattga
    72241 agagatcaat caaaaccttt atttaacgat cctttttcta cagaaaacaa gtgtaacact
    72301 tttcttgaat tttgtatgcg aagatgctta aaacagggat atatcagctt tttccttatt
    72361 tacgttttcg gatcatatct ccaggtttca ccggctgtgc gactggcata gttaaaatca
    72421 tcattggatt tggtgctcgg tcaatggatt cgccttcttc attatgcata acttcaacgg
    72481 tttgactgaa atgactgaat cctggaccat aaaattcgat ttcatctcct acagaaaaca
    72541 agtttctctg acgtatcgta gcaatctgtg ttgcctcatc ataagataat acttcaccga
    72601 caaatttata tactgggatt ttacgacgtt caccgaataa ttgttcgttt tcagttggtg
    72661 tattgtagta aaaacctgta gctaattctc gttgtgctac tttccataac tcgtcgatcc
    72721 attcttgctt gcagacatag ttttcaggat cttccatata actgtctaca gccgctttat
    72781 agacatttga caccgtagat acataatgga tcgatttcat tcttccttcg atcttgaagc
    72841 tgtctacgcc gttttcaatc aattctggaa tatgttcgat cattgacata tccactgcac
    72901 tcatcgagaa ttcttcttcc acttcgcctg tatcagttaa actgtttcgt tcttgaccaa
    72961 aaggcatatc gaacaatcca tacttccagc gacaagattg agaacatccg ccacggtttg
    73021 catcccgcat cgacatatgg ttggacaatg tgcatcgacc agaataagag atacacatgg
    73081 caccatggat aaatgcttcg atttcggtat ccgtattttt acgaatctct gccacttcct
    73141 ccattgaaac ttcacgagct aaaacgacac gttccaaccc ttcttctttc cagaattcca
    73201 atgtctcaaa attagttgct gaagcttgag tggataaatg gatcggcaga cctggggctt
    73261 ctgtcgcaca aatttcgatc aaagctggat cagaaacaat cactgcagaa ataccgacat
    73321 cacgcaaact gcggaaaaat tcccctgctc catcttcatt tccttcatgg gtgaccatat
    73381 tagcagcaac gtataccttc gcatcatgtt cttttgcaaa agcaactcct tcagccattt
    73441 cttcataagt aaaattacct gcacggcttc ttaaaccata tgcattcccg ccaatatata
    73501 ctgcatctgc tccataatgg atcgcggttt ttaacttttc cagtgttcct gctggcgcta
    73561 ggatttctgg tcgtttcaat tttcttttta ctgtcatttc tttcatcata ctccttattt
    73621 gatagcagaa ggatcaatat aaaagaatcc tgtatctaat tcacggtttt caggatgcaa
    73681 tttactgatt tcgctagtca ttttttccgc tttttcttga tcccagctac cttctaaaat
    73741 ttgttttttt gcttcggcga atagtcgagt gatgtttacg aatttttcgc ctggagtaaa
    73801 taatccgtcc aatttccatg tacagtaatg atgctgataa agttcattca attcattcga
    73861 gagattcaga tcattgctgg caaaaatatg tgtgccatga ctatcttcat aaatagaata
    73921 atgtgtctct tcttttttag gttccgagat aaaaagacct ctttcacgat ctttttgttc
    73981 gtcttgtttc gtatagttat agtaattctg tagcagtgga cgtttggatt gatggatgca
    74041 tgtagcacca tacacaaggg tttctacagg aatatcgagt ttttcttcca tcgccttcat
    74101 ctcttcaaaa ggaacttcac gagcaagtac tgctccaact gcgccttttt tcgcccagaa
    74161 attgatttga cgtgcgctcg ttactaatgt ttcagcatca taaatatacg gtatacgcaa
    74221 ttcaggattt tttttcatcg tataaataat acctgtatca ccaactgtga tcacatctac
    74281 gtttatttct tttaaaaaag ccaaatattc aggtacattt ttcatttttt caggatgaat
    74341 gatcccattt acagcaatat tcgctttttt accatacttg tgtgctaatt ctaccaattg
    74401 tcgctgttct tctcgtgtaa atgaagcagg cagacgcaat ccaaattgct cttcaccgaa
    74461 atatagtgta tctgcacctg cagcaagcaa ctgttctgct tgttcaatag tttctactgt
    74521 ggttatgagt tcgatcataa ttttctcctc ctacaacttc ataatagtag catagctcta
    74581 aaaactttcc aatacttctt ctatttaaag tcggattttt gaaaattatt tcacaaaaaa
    74641 tcaaaataaa gttcgatata ttctgtccaa atataaaaag agattgtgac aaaaatcttg
    74701 tcacaacctc ttaatcaagt cttactatta taaaaattca gctacacagg ccaagtaact
    74761 atagggaacc tactgtttcc tttttaggtc cgcgaacatt caatgtgatc ttaccttttg
    74821 aagcacctac tgttacgtgg tcacctaaac gaatttctcc gccaagcaaa gcctcgctca
    74881 atcgatcttc aatttctttt tgaagtgcgc gacggattgg acgtgcacca tattcaggat
    74941 caaatccagc tttcccgatc acatcgattg cagatggtgt gattttcact tgaatatctt
    75001 gttctttcaa tcgtttgata atcgctttgc tcatgatttg gacgattgta tggatttcat
    75061 ctttactcaa tgaatggaag acgacagtct catcgattcg attcaagaat tcaggtcgga
    75121 aagctttctt taactcttct aagatacgtt tttgcatggc tttgtgatct ttagtaacat
    75181 cttggacatt gaatccgaca tttttttctt ctcgaatttg tgtcgctccg atatttgaag
    75241 tcataatcat gattgtattt ctaaagtcca cttttctacc tttagaatct gttaaatgac
    75301 catcatccaa tacttgcaga agtaaattga aaacgtctgg atgtgctttc tccacttcat
    75361 ctaagagaat cactgaatat ggtttttgac gaattttttc tgtcaactgt ccaccttcat
    75421 cataccctac gtatcctgga ggagacccga ttaatcggct tgtactgtat ttttccataa
    75481 attctgacat atcaacacgg atcagggcat cttcacttcc aaacatgact tctgaaagtg
    75541 ctttggccaa ttctgttttt ccgacaccag ttggtccaag gaacatgaat gaaccaattg
    75601 gacgatcagg atctttcaat ccgcttcgtg cacggcggat cgcacgtgaa actgctttga
    75661 cagcctcatc ttgaccaacg acacgttcat gcagaagtcc ttccagttct aacaagcgtt
    75721 cgctttcttt tttctccaac tgctgtagag ggacaccagt ccactccgat acaactgtag
    75781 caacatcttc ttctgttaca cgatcagaat aaccttgctc ctgttttgct tcaattgcag
    75841 aaactttttg cagcttttta cgtagtgctt tttcttgtct gcgtaattga gcagcatttt
    75901 caaagtcttg tttttggatc gctgcttcct tttcttgtac tagttcatcg atttctagtt
    75961 tgatcacagt tgattttgtt aagtgatcgg tttgatccaa acggactttc gctgctgatt
    76021 catcgatcaa atcaattgct ttatcgggta gttgacgaga agtgatataa cgaacagaaa
    76081 gattgactgc tgctcgtaca gcttcatctg tgatctccac tccatggtgc tcttcatatc
    76141 ttgtacgtaa tccttgaaga atcacttctg cttcttcagg agttggctca tctacttgaa
    76201 tgcgagcaaa acgtcgctct aatgcagaat ctttttcgat atatttttga tattcatcta
    76261 gggtcgttgc tccgatcgtt tgtaattctc ctcgagctaa cgccggtttc aagatattcg
    76321 aagcatcaat tgctccttct gctccacctg caccaattaa ggtatgcaat tcatcaatga
    76381 ataagattac ttgaccatca ttatagattt cgtcgatgac ctttttcatt cgatcttcaa
    76441 attcaccgcg atatttcgta ccagcaacca aagatcccat atcaagcatc atcaggcgct
    76501 tttcttgcat gtcttctggc acttcaccta aaatgatttt ttgagctaac ccttcagcaa
    76561 tggctgtttt gcctacacca ggttcaccca ccagtacagg gttattcttt gtccgtcggc
    76621 taagaatctg gataagccgt ttcacttctc tcgaacgacc aacaactggg tccagcttgt
    76681 tttcttttgc taatttcgtc aaatcacgtg ctaatgaatc tagcgtaggt gttccttgcg
    76741 gtgtttgccg tttatcttgt acatttacat ttctacgacg attcattcct tttccagcag
    76801 aatcaccagc tagccccatt ttttgtttca gaacttgacg ggcttttgaa aggctgatac
    76861 ctaagttgat catgattcga gtagccatga tactttcatc ttttaataag ctcatcaaaa
    76921 tatgttctgt tccaactttt tgagaaccag ttttcttcac ttcgctgcta gctaaagcaa
    76981 aaacttgttt cattcttggt gaatatggaa gaaagactcc tttagggtaa gaacggatac
    77041 caccataacc aatcagatgt tcaatttctt cataaatttc cgtttcattc aaatccattt
    77101 cacgtaatac ttttccagca attccatttt gctcgaccac taaggctagt aaaacatgtt
    77161 ctgaactaac tgattgatgt ttcaaccgtt ttgcttcttc ttgtgcaatt tctaatactg
    77221 cttgggctct ttcagtaaaa agttcttcca tctaatctca cttcccatct tcataactta
    77281 aacgttccag aaatgctcgg agaatccgag cacgtgtatg atcttcatat tcattgaaat
    77341 tcaatgtgtt ttttgcaatt gcactcaaca tcaagtttcc ttcttttttt gtaattattt
    77401 ggtcttcata aagtttttga ataatagcaa aagcattttt ctcactgatt gtatcatcaa
    77461 ataactgatt tatctgctca agtaattgtt ttttatctga aatccttact tttgcaattc
    77521 gtatataacc accaccacca cgtttgcttt ctactagata accacgttgg atggtaaaac
    77581 gggtattaat cacatagttg atttgtgaag gaacacagtt aaagaggtct gccatttcaa
    77641 tccttcgaat ctcgatcatt tcactatttt ccagaatttt ctttatatat gattcaatta
    77701 aatccgaggt attctgatgt gccatcttat tcattccctt tgactaatat tgaccttaat
    77761 tataacgaat ttgataatag aaagaaagaa gaacgctttt tgttaaataa atcctttaga
    77821 atcaacaaaa aaaaatacgg aatatactat tccgtatcga cgcgcccaag aggagtcgaa
    77881 cccctaacct tttgatccgt agtcaaacac tctatccagt tgagctatgg gcgcatattc
    77941 aatttaaaaa aattataaat catttgaaaa caaaaagcaa gataaaaaaa atcgggaaga
    78001 caggattcga acctgcgacc ccttggtccc aaaccaagtg ctctaccaag ctgagctact
    78061 tcccgtttaa aaaataaaaa agac
//
LOCUS       DASHHB010000006        73509 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.006, whole genome shotgun sequence.
ACCESSION   DASHHB010000006 DASHHB010000000
VERSION     DASHHB010000006.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 73509)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 73509)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..73509
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.006"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(26..1006)
                     /gene="pta"
                     /locus_tag="U2K71_000448"
     CDS             complement(26..1006)
                     /gene="pta"
                     /locus_tag="U2K71_000448"
                     /EC_number="2.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acetyltransferase"
                     /protein_id="HEM7314563.1"
                     /translation="MELFDSLRFKIVRRGIKIVFPEATDARILGAAARLKAEELVEPI
                     LVGNEEEIRNAAHARGIKTSSFTIISPDNYDKWDEMVDTFVERRNGKASKEQAEKILK
                     DVNYFGTMLTYMGIADGMVSGAIHSTGDTVRPALQIIKTKPGISRTSGAMIMVRGRDQ
                     EKYVFSDCAINVNPTAQELAEIAVDSAKTAELFDIEPKVAMMSFSTKGSAKSPEVDKV
                     VEATKIAKKLAPQYMIDGELQFDASYVPSVAQLKAPDSEVAGQATVFVFPELQSGNIG
                     YKIAQRFGNFEAIGPILQGLNKPVSDLSRGANEEDVYKLSIITAAQTLID"
     gene            complement(1159..1839)
                     /locus_tag="U2K71_000449"
     CDS             complement(1159..1839)
                     /locus_tag="U2K71_000449"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HEM7314564.1"
                     /translation="MKEIIHNSWQEVLSSEFSKDYYLHLREFLKKEYASQKIHPDMYH
                     IYEALELTPYEEVKVVILGQDPYHGENQAHGLSFSVQPGVKIPPSLRNIYKELYDDLG
                     IAPVQHGNLVSWAKQGVLLLNTVLTVREGQAYSHRGKGWERLTDTIIEKLNEREKPIV
                     FILWGKPAQEKIKMIDKSRHIIITSPHPSPLSASRGFFGSKPFSKTNDALLALGEEPI
                     DWQLPETV"
     gene            1965..2834
                     /locus_tag="U2K71_000450"
     CDS             1965..2834
                     /locus_tag="U2K71_000450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM7314565.1"
                     /translation="MIKLIASDMDGTLLDAHMSVSPENAEAIRFANDAGVEFMVATGR
                     NYQEARAALDEVGIDCAMITLNGAQVFDKEGNSLFTVSIPNNQAISVLDILDANGIYY
                     EVATNDGLYSENQAKRIESFASMVATHLPHLTYKMAIAMASAKLELLHITYVDSIRAI
                     LEEKNLEVLKIICFHTEGPAVLGPVGKQISLLGDLAVTSSGQNNIEVNHKNAQKGIAV
                     AHVAHERGISLEEVMTIGDNFNDVSMLQVAGVSFAMGNAEIEVKDYAKYTTDTNLESG
                     VGKAILRAIAEDL"
     gene            2860..3375
                     /locus_tag="U2K71_000451"
     CDS             2860..3375
                     /locus_tag="U2K71_000451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314566.1"
                     /translation="MSEFFIQEKQLGRATRTIVKNEKGHSLFLMVGRWGTKGDALSLY
                     AMNGDLVAHIKQISLTFGTRFELYKEFEKVGTMQKIFNWPGDFYYIRRLHWTVQGDIY
                     NHQYQIHHFNQQIMKMDKATLFSGDYYVLDVSNDEDAPVCICIAAVLDYWLYRKDKSR
                     DKPVSYRLNLS"
     gene            complement(3422..4015)
                     /locus_tag="U2K71_000452"
     CDS             complement(3422..4015)
                     /locus_tag="U2K71_000452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7314567.1"
                     /translation="MLEWRKGEETMIRFARKEDAPEIAPLILVILKDMELPFLKQTGE
                     QKTLEILEEAITDPNYRYSYSRGIVEEIDGKVAGVAYGYTDEEESIIDQPLVPILEKY
                     QLDASTRLFIDKETFPNEWYLDSISVSEDFRGQGIGSRLLEALPKLAKKANRSVIGLS
                     VDEKNPKAKKLYERHDFKVVGQRMISGHLYDHMQKNI"
     gene            complement(4040..4219)
                     /locus_tag="U2K71_000453"
     CDS             complement(4040..4219)
                     /locus_tag="U2K71_000453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314568.1"
                     /translation="MEKMKCPNCGKKFAYEEVNNVVEHNDKEMPVVCPYCRTEAARIV
                     THGYFITEKIEDFLK"
     gene            complement(4296..4805)
                     /locus_tag="U2K71_000454"
     CDS             complement(4296..4805)
                     /locus_tag="U2K71_000454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314569.1"
                     /translation="MKKMMFGVAALIVIGITGMTGLLNAAFHMDDISDQEYRISLKKA
                     AAIVKNETKEHNISAIHFAVEKDLENPTNATYEYQFFCEGQVIAVNPSTGKTTVRNDT
                     EKAAEHLFDIEKTEEFKQPQAAMKEAVSYYGKKAKVKEWTLLVKDERPYYKVRLIDGE
                     QDRQVWISA"
     gene            complement(4901..5280)
                     /locus_tag="U2K71_000455"
                     /pseudo
     CDS             complement(4901..5280)
                     /locus_tag="U2K71_000455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017644319.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
     gene            5610..6359
                     /locus_tag="U2K71_000456"
     CDS             5610..6359
                     /locus_tag="U2K71_000456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinamide mononucleotide transporter"
                     /protein_id="HEM7314570.1"
                     /translation="MKSYFQFLAHQLKGWPQQNYYLFFFSLGCQVMTLVNSPITWLSV
                     ITFIGTTLGVLCILSINAAKSVNGILGILSAICFIIVGFSAKNYLSIGEQIAYMITLD
                     IPVLLSKEWNHNMASKIRKFTGKSWFIAIISTFLVYLVSGFLIGRYTDDPRPWVDAIA
                     FSISLTAGIISFLRYNNQYYWWLASGLAQMVLWYISFTHGSASLAMFVNSSVYLLNDV
                     LAFTVSPWYNQKERERMQKMEEEYYNAQQHA"
     gene            complement(6543..7820)
                     /locus_tag="U2K71_000457"
     CDS             complement(6543..7820)
                     /locus_tag="U2K71_000457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314571.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
     gene            complement(7899..8543)
                     /gene="pcp"
                     /locus_tag="U2K71_000458"
     CDS             complement(7899..8543)
                     /gene="pcp"
                     /locus_tag="U2K71_000458"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEM7314572.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            complement(8742..9686)
                     /locus_tag="U2K71_000459"
     CDS             complement(8742..9686)
                     /locus_tag="U2K71_000459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEM7314573.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            complement(9688..10374)
                     /locus_tag="U2K71_000460"
     CDS             complement(9688..10374)
                     /locus_tag="U2K71_000460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEM7314574.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            10848..11168
                     /locus_tag="U2K71_000461"
     CDS             10848..11168
                     /locus_tag="U2K71_000461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEM7314575.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            11322..12227
                     /locus_tag="U2K71_000462"
     CDS             11322..12227
                     /locus_tag="U2K71_000462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7314576.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            12224..12997
                     /locus_tag="U2K71_000463"
     CDS             12224..12997
                     /locus_tag="U2K71_000463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM7314577.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(13178..14602)
                     /locus_tag="U2K71_000464"
     CDS             complement(13178..14602)
                     /locus_tag="U2K71_000464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7314578.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            14813..15259
                     /gene="nrdI"
                     /locus_tag="U2K71_000465"
     CDS             14813..15259
                     /gene="nrdI"
                     /locus_tag="U2K71_000465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEM7314579.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            complement(15324..17306)
                     /locus_tag="U2K71_000466"
     CDS             complement(15324..17306)
                     /locus_tag="U2K71_000466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM7314580.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(17320..18216)
                     /gene="murQ"
                     /locus_tag="U2K71_000467"
     CDS             complement(17320..18216)
                     /gene="murQ"
                     /locus_tag="U2K71_000467"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEM7314581.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            complement(18219..19280)
                     /locus_tag="U2K71_000468"
     CDS             complement(18219..19280)
                     /locus_tag="U2K71_000468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEM7314582.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            complement(19491..20057)
                     /locus_tag="U2K71_000469"
     CDS             complement(19491..20057)
                     /locus_tag="U2K71_000469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM7314583.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            complement(20085..21587)
                     /gene="eat(A)"
                     /locus_tag="U2K71_000470"
     CDS             complement(20085..21587)
                     /gene="eat(A)"
                     /locus_tag="U2K71_000470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEM7314584.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            complement(21845..22966)
                     /locus_tag="U2K71_000471"
     CDS             complement(21845..22966)
                     /locus_tag="U2K71_000471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEM7314585.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            23092..24033
                     /locus_tag="U2K71_000472"
     CDS             23092..24033
                     /locus_tag="U2K71_000472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEM7314586.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            complement(24183..25079)
                     /locus_tag="U2K71_000473"
     CDS             complement(24183..25079)
                     /locus_tag="U2K71_000473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEM7314587.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            25348..26688
                     /locus_tag="U2K71_000474"
     CDS             25348..26688
                     /locus_tag="U2K71_000474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7314588.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            26906..27349
                     /locus_tag="U2K71_000475"
     CDS             26906..27349
                     /locus_tag="U2K71_000475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEM7314589.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(27853..29271)
                     /gene="nhaC"
                     /locus_tag="U2K71_000476"
     CDS             complement(27853..29271)
                     /gene="nhaC"
                     /locus_tag="U2K71_000476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEM7314590.1"
                     /translation="MGKKGTPSFLEATLVLMVIVLGIAIGVVGLKVSPNITILAAIGM
                     VMLYAVIKRYPTDWLHEGIINGIKPGIIPIFIFILVGSLIAVWIQAGIIPTLMVIGFK
                     LISVQWFVPSVFVVCAIVGSAVGSAFTVMSTIGIAFFGIGTTLGLNPALVVGAIVSGA
                     VFGDKMSPLSESTNLAAAIVDADLFKHIKHMMWSTVPAFVVSFFLFMILGHTSKAASL
                     SSIREVTDVLEANFTISIWSLIPLFLMLLCAWKKVPAILTILLNIAVAVGMIFIQNPQ
                     TSLTKLAGVIESGFVSNTGNQQIDSLLSRGGIESMMPTVSLIILTLSLGGLLIEFGLI
                     STVMERVSRKMTNTPNLIFTTLMTSIGVNIFIGEQFLSVILPGNAFKETYEKAGFDPT
                     VLGRTLEDGGTVINYLVPWGIAGSFVASTFGIPTLTYLPFVFFSLLSPVFSMVSAFTG
                     WGISKKFVKPQEAVSKTAQLEQ"
     gene            complement(29674..30897)
                     /locus_tag="U2K71_000477"
     CDS             complement(29674..30897)
                     /locus_tag="U2K71_000477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallopeptidase"
                     /protein_id="HEM7314591.1"
                     /translation="MTTTISTNQLKDLIQYRLPEYQALALDIHEHPEVSNYEFYSSAA
                     LIKQLEKERFWVEKEVAGHRTAFDARYISNKPGPTIAFLAEFDALPGIGHACGHNLFG
                     TYSVLAASIVKQFIDETGGEIRVYGTPGEEGGENGSAKGSFVREGFFEDVDVALCVHP
                     AFRYGKTTESLANDPVDIKFYGVASHAAAAPEKGVNALEALIQVFNGINALRLQLPKD
                     VNIHGIITDGGVAANVIPDYAAGRFYLRAGNRQTLDKVYEKVENIVRGAAISTGTTFE
                     FGLFQNAVDDVIVTPSFDDLFFQHAKEAGVPDEEIETEQRTSLGSSDVGNVSQVIPTI
                     QPTVAISETYIAGHTEEFKAAAKSDKGLASIAIAAELLANTALDLLKEPKLLERIKQE
                     HQEIVARKKQEGATF"
     gene            complement(30928..31767)
                     /locus_tag="U2K71_000478"
     CDS             complement(30928..31767)
                     /locus_tag="U2K71_000478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MetQ/NlpA family ABC transporter
                     substrate-binding protein"
                     /protein_id="HEM7314592.1"
                     /translation="MKKIIRFVVITFVAIIWLAGCSQGKADTTDKKETIVKLGIIGED
                     TDVWDNVKDRLKKENINLEYVKFTDYNQPNVALADGSIDLNSFQHQFFLDNYNKEHGT
                     DLVSIGNTVNAPLGIYSNTLKDVADIKDGAKVAIPNDVTNGGRALLLLQTAGLIKVDK
                     AKGQAPTVSDITENRKNLDISELDASQTARALSDVDISVINSGVAVDAGFNPASDAIF
                     LEPVDDHARPYVNIIVARKEDENNKTYQKIVDAYQTEDTVKVIEKTSKGSSVPAWETF
                     GRK"
     gene            complement(31784..32464)
                     /locus_tag="U2K71_000479"
     CDS             complement(31784..32464)
                     /locus_tag="U2K71_000479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314593.1"
                     /translation="MTDFFKTYLPNVYLIPDEFVEATKQTLYMSFWTAFIGGMIGIIL
                     GVTLVVTRPNGLLANRLLFEILDKLINIIRSIPFIILLSLLALTTRFLVGTTIGAKAA
                     LVPLIFGVVPFFARQIENALLEVDPGVIEAAEAMGTSPIGIILRVYLLEGLPGIIRVS
                     ALTIINVIGLTTMAGAVGAGGLGNLAISRGYNRFQTDVTIVATLLILILVFLSQFISN
                     QLIKKITH"
     gene            complement(32466..33539)
                     /locus_tag="U2K71_000480"
     CDS             complement(32466..33539)
                     /locus_tag="U2K71_000480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7314594.1"
                     /translation="MIELRDVSVCFKQKEKEIQAVDSVDLTIEKGDVYGIVGYSGAGK
                     STLVRVMNLLQKPTAGEVIVNQTNLSQLSPKELRKERKAIGMIFQHFNLMESRTIFDN
                     VDFSLKYSGKSKQERRQKVNELLELVGLEEKASAYPKQLSGGQKQRVAIARALANEPK
                     VLLCDEATSALDPKTTLQILALLKKLNRQLGLTIVLITHEMQVVKEICNKVAVMEDGR
                     IVEKGSSIQIFSNPEEELTKDFIRTATHLDQALETIIAHSAFADQIANKWLVELSYIG
                     NQTNEPLIAHLYSKYQVTANILYGNVELLQETPLGSLIVTLAGETKQRKKALDYLIKS
                     GVKINILQKNDLDSRIKVIEGGR"
     misc_binding    complement(33595..33833)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     misc_binding    complement(33876..34109)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(34186..34857)
                     /locus_tag="U2K71_000481"
     CDS             complement(34186..34857)
                     /locus_tag="U2K71_000481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM7314595.1"
                     /translation="MKGAIFDLDGLLVDTEKTYRDGWLWGFQQFDIHIPKEIVDAWGG
                     KNWKQSFDILVKAAGSPEKVQEVRAKREEYFYQQLTSGAIHLKPYAKEILADLKERHM
                     KLGIATTTVTKRATDILDHFKLADYLDTFTFGDEVSENKPSPIPYLTALERTGLEASS
                     AFAVEDSLVGATAASRAGLGVVLIPDSSFVRNYTVEEKEKLKLLAEGKDLRTVIEMLN
                     KKTEK"
     gene            complement(34944..37622)
                     /gene="mgtA"
                     /locus_tag="U2K71_000482"
     CDS             complement(34944..37622)
                     /gene="mgtA"
                     /locus_tag="U2K71_000482"
                     /EC_number="7.2.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium-translocating P-type ATPase"
                     /protein_id="HEM7314596.1"
                     /translation="MSFGHKGEIEMEKKKDTQKIYAAFAGKTKQELFELFHASANGLT
                     EEQIERSRENYGENAISYGKKTPFIVEILKAYITPFTLVLIALGIISFITEYVLAAPG
                     DKDLFGVAIIFTMVIVSGTMTLIQSVRSNQAAEKLKSLVKVTAAVKRNGKYTEIPMEE
                     IVCGDLVRLSAGDMIPADMRLLSAKDLFISQAALTGESYPVEKHAKVCEDDQQSETSY
                     ENLVFMGSNVISGSAEGIIVSVGNETLFGHVAETLSEKPIQSSFELGIHKTSMLLIKF
                     MSLMAPAVIVINGLTKGDWLEAFLFGLSVAVGLTPEMLPMIVTTNLVKGASVMAKKGT
                     VIKNLNAIQNFGAIDVLCTDKTGTLTQDKIILEYHMDCEGNEDDRVLRHAYLNSYYQT
                     GLKNLLDVAIIDEATETLDTDKINYQKVDEIPFDFERRRMSVVVADPAGKTQMITKGA
                     IEEMLNISKFIDLGGTVTPLTKERKEAVLAKVQSLNEDGLRVIGVAQKTNPSVVGEFS
                     VKDESDMVLIGYLAFLDPPKETTKQALKALKEHGVAVKVLTGDNELVTRSVCRQVGLE
                     INELVTGEKISEMSDHELAQVAEDHEVFVKLNPQQKARLTTALRKNGHTVGFLGDGIN
                     DAPAMKVADIGISVDTAVDIAKESADVILLEKDLTILERGLLSGRETFGNIMKYIKAT
                     ASSNFGNMFSVLIASTFLPFLPMLPLQLLFLNLIYDVSCISLPWDRMDKEYLDEPKKW
                     NASSIGEFMVYFGPTSSIFDITTYLLMYFVICPAVVGGSFHTLDSSQRVIFIALFHAG
                     WFVESLWTQTLVLHALRTPKVPFIQSNATFAMFVITTLGIIVGSVLPFTSFGAELGLM
                     PLPGNYWLWLFATVIAYLALVTAVKKIYIRRFQELL"
     gene            38281..38718
                     /gene="ndk"
                     /locus_tag="U2K71_000483"
     CDS             38281..38718
                     /gene="ndk"
                     /locus_tag="U2K71_000483"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HEM7314597.1"
                     /translation="MERTLVIIKPDGVRRHLVGSIIQRFEAKGLAIAEMKFETMTPEL
                     AKEHYQHLTERSFFDELIDYMTSGPVVYLVLVGEEVIDIVRKMVGATKAADAVPGTIR
                     GDYALPGTENIIHASDSRDAAVKEIARFFPPFDINKPSEKAAK"
     gene            complement(38740..39606)
                     /locus_tag="U2K71_000484"
     CDS             complement(38740..39606)
                     /locus_tag="U2K71_000484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM7314598.1"
                     /translation="MYDGELIKKLRVNKKLTQSQLAEGICFKTSLVGIETNSVKKMSF
                     VTLRSFLERMNMTLAEYEWMRNQLDEPIKLKKKRRMLDRVQEDSFDPYKEISNNRRHF
                     KKTEDLYYLVLNLHMVEKTEVSETEFEFLRYECRIIEEYLMQIPDYGQFELSILADFP
                     WIFSDRFIENNYLKIKKRMRRLAQLNNHEQYLFTFLMNLTSFYIENGRLKKARSVNED
                     MKRSLSHKERSTVIYETLMADYYQRLISAALGEEKTKDYQEFFTVLEYMLGKNQRRSL
                     EKKLLAIAEPSN"
     gene            complement(39766..41859)
                     /locus_tag="U2K71_000485"
     CDS             complement(39766..41859)
                     /locus_tag="U2K71_000485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM7314599.1"
                     /translation="MTQLKKLCQNRLAIVLTAVFFFWLKTIIAYYADFSLGVEGTIQY
                     FILWINPIATTLLFFGLSLYIKKPKPALAILLIIDILNTLLLYLNIIFYREFTDFITV
                     KSVLGFSKVSQGLSGSSFSLMKPHDVIYWLDIAVFIGLLVWLKVKKIPIKSNPVGKPM
                     AIAVTCLSGLIFSGNLALSEANRPQLLQRTFDRSYIVKYLGIDAYTIYDGIKTGMTSS
                     VRAHASSNGIDEVLDYTKKHYAEPNPETFGIAKGKNVIVLHLESFQQFLINMKVDGQE
                     VTPFLNSIFQNQATISFDNFFHEVGQGKTSDAENMLETGTFGLPQGSLFTELGSDNVF
                     QAAPAILGQKQGYTSAVFHGNVASFWNRDHVYKNLGYDNFFDRSYFDESDETLGYGIL
                     DKDLFRESAQYLEHLQQPFYTKFLSVTNHTPYYTDDKNFDFPSLNTGNSTVDNYVRTA
                     HYLDQSLEQFFTYLKKSGIYQNSIFVIYGDHFGISNTDNKDLASALGKDPDTWDEFDN
                     AQMQRVPLMIHMPGYTKGTVNHEYGGEIDVLPTLLHLLGIDDKDYLHFGTDLLSSQHD
                     QVVAFRNGNFVTPKYTVLGGKAYNNQTGEIIDTKAAGIDEEISKDQEKVKTALSLSDK
                     LNQENLLRFYVPNGFETIDPKKYDYKNEAERNEAIEKQKGEHSTSVFSKNGNKTTTNL
                     YPVTRTDVEQQKNNQ"
     gene            complement(42608..43231)
                     /locus_tag="U2K71_000486"
     CDS             complement(42608..43231)
                     /locus_tag="U2K71_000486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314600.1"
                     /translation="MRKKRPFLLFWLFGFFLLLILSAAFSGKMTADLAQKETEKIVAS
                     KESVDNFKKEFSTYWWAEYKITANYEKGELDIYLPKGKEENQFINIDDVLSAISLRQY
                     QFKDKHLTVLLLTHDGDKVARLKNGKVITAYSEKVDKKVVADRLAEWKKTNEQVKKTT
                     VSSQKNESTGTSPSDSGSGQTITGSEENQASIITNESSQGQYQSYGQ"
     gene            complement(43390..44787)
                     /locus_tag="U2K71_000487"
     CDS             complement(43390..44787)
                     /locus_tag="U2K71_000487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM7314601.1"
                     /translation="MKKYQFPANFWWGSAASGPQTEGRISGDGKGENIFDYWYKKEPQ
                     KFFDQVGPEKTSQVYTKYQEDVQLMKQTGHNSFRTSIQWSRLIPDESGTINEEAVRFY
                     HRYFDELIENGIEPFVNLYHFDMPMYLQEKGGWLNRETVEAYEKYAKSCFELFGSKVK
                     KWFTHNEPIVPVEAGYLYRWHYPEESDMKKAIQVGYHEALASALAIKSYHEMEQGGVI
                     GIILNLTPSYPRDESNPEDVKAAQIADAFFNRSFLDPAVKGEFPSELIEIVKELDLMP
                     VIHAEDLTVIKENTVDLLGINYYQPRRVKAKETPIDTKNGPMPEDYFDNYEMPGRKMN
                     PYRGWEIYEQGIYDILINVRDHYGNIDCFISENGMGVENEARFIKEDGMIHDDYRIEF
                     VQSHLKYVHQAIQEGANCLGYHMWTCMDNWSWTNAYKNRYGFISVDLAQDGKRTIKKS
                     GHWFKDVSDKNGFEA"
     gene            complement(44819..45790)
                     /locus_tag="U2K71_000488"
     CDS             complement(44819..45790)
                     /locus_tag="U2K71_000488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7314602.1"
                     /translation="MASIRDVAKMAGVSVGTVSRYLNGQQLKEKNMKKIAEAISALDY
                     KENIIAKGLKNNRSFSIGLLINGISSRFGSEVVSGIERVAEENGYSLLLSGFADQPEK
                     IEQKIEYLMKHVIDGLIVFLSEEEWSGFEMLTKMSIPVIALNCPEGPAGIDTILVNDR
                     ESVAKVIAHHGEQGHKKIGFIAATQTDYVARERLAGAKEAVQNDPSIQLEVFTGDYSR
                     KSGYYGAKALLEKGATAIFVSNYNMSIGAIECFNQEGVRIGKDIAFSHYDYLDKNDLS
                     ILPKITITPPTDKIGERAAEQLLKRIKKEEEENEKVIVMENIIHGIN"
     gene            46164..46589
                     /locus_tag="U2K71_000489"
     CDS             46164..46589
                     /locus_tag="U2K71_000489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314603.1"
                     /translation="MKKLIVLFILSAGLLTLTGCDLFGGSDQSSDETTDSSVLKAEKA
                     DTTNSVYTLKVRKDMDLSAGQLTFTDKKMEWTRTYQTQGTDSSESSEEKTVLTDIKIT
                     TKDDEYIISGKENKKETTVIFTKIGNYRLKDEDGNIYSL"
     gene            complement(47077..47778)
                     /locus_tag="U2K71_000490"
     CDS             complement(47077..47778)
                     /locus_tag="U2K71_000490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEM7314604.1"
                     /translation="MSVILISGTIGAGKSSLTDMLAKEIDSKPFYENVEDNEVLPLFY
                     SNPEQYAFLLQIFFLNKRFLAMKNALVNDDNVLDRSIYEDSLLFHLNADLGRVTDIEV
                     QQYDSLLDTMLNELDDVAPKKRPDLMVHIKVSLDTMLERIKKRGRDYEQLESDETLYT
                     YYETLNTRYNQWYEDFDVCPKIQVDGDKFDFVENPEDAKKVIRQIQEKLAELEGKPAG
                     KYFTDNQHAKGQELG"
     gene            complement(47794..48234)
                     /locus_tag="U2K71_000491"
     CDS             complement(47794..48234)
                     /locus_tag="U2K71_000491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEM7314605.1"
                     /translation="MSQIYLAGPFFSEEQIDRVSRIEKALEENKTVTSFYSPRHHQES
                     NYELFSAGWAQEVYEKDMEELTNAEFVVAILDFEHQTIDPGTAYELGVATMLKKPMII
                     VQEETVPTNLMITQSLHTYLKSDQAVREYDFETLPVETYVGEYL"
     gene            complement(48234..48809)
                     /locus_tag="U2K71_000492"
     CDS             complement(48234..48809)
                     /locus_tag="U2K71_000492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HEM7314606.1"
                     /translation="MKLTVFCGSRFGNKESYKFIAQTLGKYMAQEEIELVYGGSYSGI
                     MGVISKTVLENNGKVTGIYPNGLFETELPGKDVTTFIPTETIDERKVLLFEKGDAVLV
                     FPGGLGTLEEFSQLLSWIAIGLTPDKPIGILNIGGYYSGLQQLLETFAKEDFMDKKWL
                     DQVIFSNNPLELVNELRAVSIKNQLLLKEAK"
     gene            complement(49657..50136)
                     /locus_tag="U2K71_000493"
                     /pseudo
     CDS             complement(49657..50136)
                     /locus_tag="U2K71_000493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339057.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
     gene            complement(50295..50996)
                     /locus_tag="U2K71_000494"
     CDS             complement(50295..50996)
                     /locus_tag="U2K71_000494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM7314607.1"
                     /translation="MKRTRVLYLEVADKIKEDIFSGKYPVGSMLPTESELEELFQVSK
                     ITVRKAIELLASDEYVAKKSGRGTTVLSNRPYNRLSKAASFTQILENSRYEVRKETLD
                     LQLVHIKKEDPLFSFFGEQAVCFKRLYYLNNQPYIYFVHYLPQEMMQFSREEFGKESL
                     YRLLTKKGYTIDSFSDDFSAVFLTEEEQGILKTDAQIGIKRIRKSSTQIGEMIEYSEA
                     VYHTELHPYHIDYET"
     gene            complement(51104..52513)
                     /locus_tag="U2K71_000495"
     CDS             complement(51104..52513)
                     /locus_tag="U2K71_000495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HEM7314608.1"
                     /translation="MEQLLPNQLEGAYDRDGKGLSVADAMPGGKQRFAIIGSEEFDWT
                     IDQEKYIYPNHRGIDHYDRFKEDLALFAEMGFKCYRFSIAWTRIFPNGDEGTPNEAGL
                     EFYDQLIDECLKYDIEPVITISHYEMPLHLAKEYGGWKNRKLIDFYERFAQTVLERYS
                     SKVKYWMTFNEINSAFHFPALSQGLVKSNGAGEYQNIFQAWHNQFVASSKAVKIGHEL
                     RSDIQIGCMIIYATTYSIDANPVNQAATMIQNQEFNFFCTDVQVRGEYPAYTARTHKK
                     YGVDPEKLEQTEKDFQLLKEYPVDYIGFSYYMSTAINETDPQAATSEGNLLGGVKNPF
                     LEASEWGWQIDPEGLRIALNELYNRYQKPLFIVENGLGAIDHMEEDGTIIDDYRIDYL
                     RRHIEAMANAVADGVDLMGYTPWGCIDLVSASTGEMSKRYGFIYVDLDDEGNGTLNRS
                     KKKSFDWYKEVIASNGVNL"
     gene            complement(52561..54426)
                     /locus_tag="U2K71_000496"
     CDS             complement(52561..54426)
                     /locus_tag="U2K71_000496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM7314609.1"
                     /translation="MDNQAVGRRVWEAVGGQKNVKSLVHCATRLRFRLKDESLADTQK
                     LKEDPDVIQVVQSGGQYQVVIGSNVADVYQSIVDEEGLAVSEDGKEESKNILNRLIDI
                     ISSIFTPFLGAMAAAGVLKGFLSLATVMGWLTADSGTYQILFAAADGVFTFLPVMLAF
                     TAAKKFKANQFLAVAIAMALVYPAITAVAGAGEAISFFGIPVILSPSGYTSSVIPIIL
                     AVWVQSKLEPFVKKVIPQFLQMILVPLVVLVVMVPLTFLALGPIGTVAGNALGGLFNS
                     IYGFSPIVAGLIMGSLWQVFVMFGMHWGFVPIMFLNIEQYGFDVLMPMLLPAILAQGG
                     AALAVALRTKDTKLRALGISSTVTSLFGITEPTVYGVTLPLKKPFIAACISGGIGGAI
                     IGFSGVKAFSSSLVSLLTIPTFINTVDGVESNVTVAVIATGIAFVLAFVGTLILGFDE
                     QTQDNQLENKHANAGEPITSARHTLKSPLTGKVLPLSEVPDQVFSSGVMGKGIAIDPE
                     VGELVAPADGEITTIFPTGHAVGITTTDGAEILIHIGMDTVELNGNGFEILVKQGDLV
                     KAGDLLIRFDIEAIRAAGYSVITPVVITNTDAFADILELDQKEIIANEDVLAIVK"
     gene            complement(54547..55397)
                     /locus_tag="U2K71_000497"
                     /pseudo
     CDS             complement(54547..55397)
                     /locus_tag="U2K71_000497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338530.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
     gene            complement(55707..56420)
                     /locus_tag="U2K71_000498"
     CDS             complement(55707..56420)
                     /locus_tag="U2K71_000498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEM7314610.1"
                     /translation="MKKKGKAKRWLVNILLFLLLLVGLALIFNEQIKDYFVRETGDKY
                     AIANVTKEDLKKNNDKDVSFDFDAVEPMTTEGVMRSQMRGTDLPVIASIAVPSVSINL
                     PVFKGLDNTSLLYGAGTLSPDQEMGKGNYALASHRATNPELLFTPLENLEMGAKIYLT
                     DLENVYTYKTFFKEKVAPTDTQLLNEVEGKEIVTLITCGDMDAVTRLVVQGELESVTS
                     IKDATDDMRSAFNLETKTF"
     gene            complement(56488..56814)
                     /locus_tag="U2K71_000499"
     CDS             complement(56488..56814)
                     /locus_tag="U2K71_000499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide pyrophosphohydrolase"
                     /protein_id="HEM7314611.1"
                     /translation="MNSMEKVNQFRDERNWRQFHNEKDLAISISLEASELLELFQWKT
                     PEEVKETQLERIKEELADVLIYSYMMADNLHLDLDEIIEEKLIKNNLKYPVEKSAGVR
                     KKYTEL"
     gene            complement(56937..57164)
                     /locus_tag="U2K71_000500"
     CDS             complement(56937..57164)
                     /locus_tag="U2K71_000500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003246265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEM7314612.1"
                     /translation="MLTQQLRELEQDESIHRKAYNQVPPKVEYSLTEEGKSLREILVA
                     LSVWEEERIEKQKASREEDTLLHTHGGYLNY"
     gene            complement(57486..58739)
                     /locus_tag="U2K71_000501"
     CDS             complement(57486..58739)
                     /locus_tag="U2K71_000501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEM7314613.1"
                     /translation="MVATVTLYLTFTLILDLGIYLFFSKQLLVKNSFGCIALMVVTLL
                     LPIPKVSFPYLVGLLLMFLFYFTTKNLFISCLISNLFFAITGLSFFLVFDLMDYLGKR
                     HLGTASFFALLIAFIFYLLVKSIDKQTRFLNLLFTYPTEHYFGGICILLSWGVIYLVL
                     GSVHFRSSNYLLLSLLSIVISFFSLFFSIMLISFQVKERQHQESLQLYQINEEYYHHL
                     EEFKHDYKSLLFSLKITLQENKKETLEFLEILEDHSAAIFEKPQIQQLTNIVSPAVRG
                     VFLKFLEKAESEKIPIQVVIPVEVKTIPIDLVVFIRCLSIFISNAFDHRVADQSPITI
                     NLLEEKEGSRFSIANKANPTNLTLSEMVKRGKTSKKNGGLGLYSAKKMLDVYENAELK
                     IEFSKQNHYFFVEMYLASRKSKSAY"
     gene            58800..59540
                     /locus_tag="U2K71_000502"
     CDS             58800..59540
                     /locus_tag="U2K71_000502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7314614.1"
                     /translation="MKKYFIIEDNQFHSEWIIRELVNHHIENIVQLTSISQINQLIQL
                     GTIDNHDVFFIDIDLKQSLTGIDVAEQIRKKNAHSNIVFFTAYTNFAVEIINRKILPI
                     GYIRKDRNLSEQIRQVLEQINWKEQQIVNVDKIFIDQKGGSLVLYPREILYIESIKGL
                     KNQVMVKTTTEDKLVNTSLKQLKTELEKYAYFLLLKSYCINLSQLQKIDSAHYVLEFF
                     NGDSLLVGRKIFEKTKERFHDFQKTASS"
     gene            complement(59626..60384)
                     /locus_tag="U2K71_000503"
     CDS             complement(59626..60384)
                     /locus_tag="U2K71_000503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEM7314615.1"
                     /translation="MKKTIALSTVLAGSLLLCTQSVFAENKSSATTEGTISVTKPDAA
                     NPEDVVPVPPVDPNDPDTPFTPDEPNQGTSGLLTIDQAPDLDFGTIKLGTAKTVYAAL
                     QSGTDSTGAQTQVQNFVQVTDKSGNYAGWKLSVKRTEFIGTTSDTNKLTGSKVKFKNA
                     VIRNSATNSSGMPSTVSGTGAEGIEIPADQAVDLVTAGANEGIGTWIYSLGENVEQGR
                     QSVELFVPVSNYAVDNYVSTFTWSLTDAPTEEEA"
     gene            complement(60381..61409)
                     /locus_tag="U2K71_000504"
                     /pseudo
     CDS             complement(60381..61409)
                     /locus_tag="U2K71_000504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628085.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
     gene            complement(61429..64596)
                     /locus_tag="U2K71_000505"
     CDS             complement(61429..64596)
                     /locus_tag="U2K71_000505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314616.1"
                     /translation="MNQCVKKLIASILFVFPLLMVTSVFADVEKTNESTQSENQSFDK
                     TTFSEESITSETTESQSEASTNKRQEENKTSETSEVAEQTSDSSEAALARAVSADGYS
                     EAATTEELAQLLADESVTKIRLIQPLTLDRELEIKRDIVIDFGGFAHNFGTHHIYINE
                     IPQSIEFQNFKGTAAHPGGLTPNIDGNAIIIAYMTDFWGSRYHFTGEIKFTGTLDLYD
                     GSKLGLIYAPRATVTLDGVSGVLDVQPVKEGLNAAPGKAYFCRSYQLNVINGSQLYGP
                     YLGKFYGFLDSGDETGSGKTAPGIHILSGSKVSLDYDRADGVSDEGEAVDTLPGNVMF
                     EVSGSGSEFSVNTKINITNDNNRGIIQMRGSGSKVTVSNGGKITINTATTGGFRLQGD
                     SSQIHVSSGGQISVKMAGDDDQPGNNGMRFVGKGLSLIVEGAGSVIDIDKQSGRSSAV
                     RFENGTQKLTVTNGGSLRVRNTGDGKSYVNRLNQGNQAIQFYDASGFLESPGSADFTV
                     TGEKSNIDIRADSGATVDTTGDIDLNFTAGEKTYLVMVGKTGEDKTGIISGDVLNVDL
                     ESPTYFDFQNKRIGSETNGIGGWVFQGNGDSSLKIAHSEIALWRKTGATFDNFEKDPD
                     YFSDMTDLSINGTDLGNFVSSPSAGLTNEFAQTGAGMKNYNRISANNQLPMIESLRVP
                     TNADKKIYGHVVVPEGVEAKPRDAWTGEVEVTLRITYADTTKSSVEITALTQGQTDEN
                     EGAGYNPWGEGEQGGLFEVQVPNDERLKTEDQVQVVAAKKVRGGQEVTELDSAKTTVD
                     VIPPEPANLSTTLINAASRTITGTGEAGALVSLKKNQAVLSETTIDDTGEFVLSIPEN
                     QLFAGDVLEIVLNDQAGEATAKGVQNKPSTNDEIGNHNPSDTPVVYHDAPPFAPATKI
                     VVEGGLSFLAPSKLDFGQIKATGLTQEAFGQMDIQEKLWIYDQRETKNPWILTIKLSQ
                     PFTNDAGFSMQDVLYFKKGEEYQLVNEEELEIVRGQNSTNVQADYSEYLNNGRAFKLV
                     LTKENQIAGNYSAEITWTLADAPTE"
     gene            complement(64820..65704)
                     /locus_tag="U2K71_000506"
     CDS             complement(64820..65704)
                     /locus_tag="U2K71_000506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEM7314617.1"
                     /translation="MKKVLLVVNPSSGGEQAKEFEQLAIAKLESVFDEVVVLHTKKAG
                     DAKNFTREAATEGYHSVFVMGGDGTVNEGISGIAEQEHRPNFGFFPLGTVNDLARALG
                     IPLEPEEAINHFSIESVKPLDIGKINDDYFMNVVAIGSIPESINDVDAEKKTKFGKLA
                     YFMSGIKQLASTQSYSFHVEVDGKKEEIESSTLLIGLTNSVGGFETLLPNAKVDDGKL
                     HLVYLKDSSLIDTLKTLPDLLKGVDESTKNLAYLTCEQLVVSLADEEELTTNVDGDEG
                     DKLPVTIKVLPSHLQVYC"
     gene            complement(65799..66236)
                     /locus_tag="U2K71_000507"
     CDS             complement(65799..66236)
                     /locus_tag="U2K71_000507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314618.1"
                     /translation="MEWFCKFLLFLEVIHVYGFVFLTNKKAGSTMDHLYIKYIHQFSV
                     LVLGTLIIAGIVAFIIQGRDILTVHQEFFSYAFGIPLIAIRSDNKKWKKRYYILLLFL
                     CFYLPPKIFTLLGIQCLSFSFISYIIVLIFLSFIVWMFYLVKK"
     gene            complement(66453..67364)
                     /locus_tag="U2K71_000508"
     CDS             complement(66453..67364)
                     /locus_tag="U2K71_000508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEM7314619.1"
                     /translation="MKKRFLALAIVLGTGLLSGCTNAGEKTAVSYKGGTISEQEVMDS
                     LKKMQGADSAVQQLIVYQVFEDKYGDDVSTKEIDSQYDQTKKQLGDSFDSQLKSAGYT
                     EQTFKDSIKQSLAFQEGLKKHIKLTDEDLKTAWESFHPEVEAQIIQVASEDDAKDVKK
                     AADKGDDFSKLAKDKSTDTTTKEDGGKVKFDSTTTTVPAEVKEAAFKLKDGQVSDVIT
                     STNASTYTTEYYVVKMVKNQNKGNDMDKYKKELKEIATDTKLSDSTFQNKVIGEVLKD
                     ANVKIKDKDFENVLSTFTSDSSTASSK"
     gene            complement(67821..68249)
                     /locus_tag="U2K71_000509"
     CDS             complement(67821..68249)
                     /locus_tag="U2K71_000509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314620.1"
                     /translation="MKKAVIICALGIGFVGGNAMSSEVEANSVHGEIYYKSLKPGEIH
                     FDAKGNIIKSNLPVDREISRTAINHSSGRWVYYSDTTCTRKTGHSNHYSRVYNRHGAK
                     AKVGNSIRSANATKGVWAYASATGNRTDTFSCWYNPTGWY"
     gene            complement(68277..68915)
                     /locus_tag="U2K71_000510"
     CDS             complement(68277..68915)
                     /locus_tag="U2K71_000510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314621.1"
                     /translation="MEPLIVIKGAKKIIRNKTILHLDRFTADAGDLVTVVGESGAGKS
                     TLLNILGLNDQFSSGQFYLFDQETKQLTGREKRLIKRNTISFLFQDFGLIEYETIDFN
                     LDIGLKFSNLNVSDKRTAKYEALKKVNINKKLSTPIYTLSGGEKQRIACARVLLKNSK
                     IILADEPTGSLDKRNKDKVVEILSQQASQGTLVIVVTHDPYLTKIGNKKIML"
     gene            complement(68919..70901)
                     /locus_tag="U2K71_000511"
     CDS             complement(68919..70901)
                     /locus_tag="U2K71_000511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1430 domain-containing protein"
                     /protein_id="HEM7314622.1"
                     /translation="MSGFALVSINKQVLYRTNEFFHQTDTYTPIRLPQHFIGQEEYEQ
                     LIMVFENVSSETELSYMKKTVNESNEVDTSWNFKIQPKLEMTLYYQGDVKSSHEKVPY
                     SNTSFRVTSIEESIKIKDFEGEFFLMTREKEAYERFISLFTDRFNQAFHTDYATNIFE
                     DFENSYSYSVISNTDVYNYRVYLTIGLILLFLLLSSFFLLMNKEISLLLNNDFSLHQV
                     LWCLLGKKKIIITFIIAGIIGGFIFMYYKEQFLYLFQMYVFLFSGIYGISLISLLLVE
                     KIASLREAFNYIQYILFTFGLLFSLLLTVANVELATIVFSSSQLAVSHEPPKEIAGED
                     YHVFYPVTIGKNHVDFIYSNRFASAADQELYETLNKNGSILVNVSDYLNEENEQFGRG
                     IKINLNYLKKFPLIDVSGRLIQITEKETHPVILIPERYRHTDLKNDLAQITEYYQEIS
                     EKEPKFLFIKNNQPIYTFIPSIPWIEHYPVVEILTLKNSMYLERNILSGEIYPPLKIK
                     IGSLSKERLFELIEESQLRDNLQLSFPYTQTEEIAVKVLSRSFHYLIQIFLLTFFSFF
                     LLSYVMLCIYFVYNCRKIAIFRSSGYSLFETYKDFFMMNLIKWGTTSVIFLFLIEREP
                     KYLFNIFFFSFIDFILSVVFILSTEKKSQLLLMNGG"
     gene            71081..71746
                     /locus_tag="U2K71_000512"
     CDS             71081..71746
                     /locus_tag="U2K71_000512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7314623.1"
                     /translation="MNTGEKIRTYRRNCKLTQKELGEKIGVSDKTISSWENSRTMPDL
                     EMLSLLHHTLGLPIDFPSIESATESIEPTLPDEPLVNKKSYSSKQPFSLKKTLSFFLL
                     VFCLVYLFLINYQYKNETIDRFNPFLKPQIGYTTLPVGYVDNSIERWVCNDIFGHGTW
                     LSFHAGEVEKEKNHAVIKHKGKHVMNVRLLTWEAIPSDYQEILSQDYDDALKRSPQEP
                     DRH"
     gene            73059..>73509
                     /locus_tag="U2K71_000513"
     CDS             73059..>73509
                     /locus_tag="U2K71_000513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM7314624.1"
                     /translation="MQGVIAMMSKNNTNGRNQFAMLTIDDLVPQDHLVRKIDAALDFE
                     FIYPIVEATYSDLGRPSIDPVILIKLVFIQYLFGIRSMRQTIKEVDTNVAYRWFLGYS
                     FEEKIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNLY"
BASE COUNT        21369 a        15229 c        12597 g        24314 t
ORIGIN      
        1 tttttggcta gttcgcgtgt atttgttaat caatcaatgt ttgtgctgct gtgataatcg
       61 ataatttata tacatcttct tcatttgctc cacgagacaa atcagaaacc ggtttgttca
      121 agccttgtag aattggtccg attgcttcaa agttaccgaa acgctgagca attttatagc
      181 cgatatttcc tgactgcaat tctggaaata caaagactgt tgcttgacca gctacttctg
      241 aatctggcgc tttcagttgt gcaactgaag gaacataaga agcatcaaat tgcaattcgc
      301 cgtcgatcat gtattgtggc gctagttttt tagcgatttt agttgcttca actactttgt
      361 ctacttctgg cgatttcgct gagccttttg tcgagaagct catcattgct acttttggtt
      421 cgatatcgaa caattctgct gtttttgcag aatctacggc gatttcagcc aattcttgcg
      481 cagttggatt cacgttgatc gcacaatccg aaaagacata tttttcttga tctcttccac
      541 gtaccatgat catcgctccg cttgtccgac tgatgccagg ctttgttttg atgatctgaa
      601 gtgctgggcg aacagtgtcg ccagtcgaat ggatcgctcc agagaccatt ccgtctgcaa
      661 tccccatata cgtaagcatc gtaccaaagt aattcacatc ttttaaaatc ttttctgctt
      721 gttctttgga tgcctttcca tttctacgtt caacaaaagt atccaccatt tcatcccact
      781 tatcgtaatt atcagggctg atgatcgtaa agctagatgt tttgattcct ctggcatgcg
      841 ctgcatttct tatttcttct tcgttcccta ctaaaatggg ttccacaagt tcttctgctt
      901 tcaaccgggc agctgcgcct aaaatacgag catcagtagc ttccgggaaa acgattttga
      961 tcccacggcg aacaatttta aatcttaaac tatcaaacaa ttccacaaga tgacctccag
     1021 acgtttttat atgtattatc atacactatt ccagaaaaaa agaacagttt attttatctg
     1081 tatcagtcaa aaatgctgga aaaattaagt ttttcacata caaagcgaac tgcactataa
     1141 caggaaagcg ctgttttatt acactgtttc aggcagttgc caatcaatcg gttcttctcc
     1201 taacgcaagg agcgcatcat tcgttttcga gaagggtttg gaaccaaaaa agccacgact
     1261 tgcagataac ggactgggat gtggagatgt tatgatgata tggcgactct tatcgatcat
     1321 tttgattttt tcttgtgccg gttttcccca caagataaac acgattggtt tttcccgttc
     1381 atttaatttt tcaatgatcg tgtctgttaa tctttcccaa cctttcccgc gatgagaata
     1441 cgcttgccct tcccgaacgg taagaacggt gttgagtaaa aggactccct gtttagccca
     1501 tgacactaga tttccatgtt gaacaggtgc gattccaaga tcatcataaa gctctttata
     1561 gatatttctt aacgatggcg ggatctttac gccaggttgc acagaaaaag ataagccatg
     1621 agcttgattt tctccatgat aaggatcttg tcctaaaatc accactttaa cttcttcata
     1681 aggagtcaat tccaatgctt catagatatg atacatatct ggatggatct tttgggacgc
     1741 atattctttt tttaaaaatt ctcttagatg cagataataa tcttttgaaa attctgagga
     1801 caaaacttct tgccaactgt tatgaatgat ttctttcaaa aactccacct cctaataaaa
     1861 agaataactc acattttctt caatcacaag ggacaatctt caccctcagc acttgattaa
     1921 tatggtaaac taggaacaag aagcagaaag gaacgataat cattatgatc aaattaattg
     1981 catcagatat ggacggcaca ttattagatg cccatatgag tgtttctcct gaaaatgccg
     2041 aggcgatacg ttttgcaaat gatgccggtg ttgagttcat ggtcgctact ggtcgaaact
     2101 atcaagaagc tcgcgcagct ttagatgagg taggaatcga ttgtgcgatg atcactttga
     2161 acggggcaca ggtatttgat aaagaaggaa attcattgtt taccgtgtcg attccaaata
     2221 atcaagcaat ctctgtttta gatattctag atgctaacgg tatttactat gaagtagcaa
     2281 ccaacgatgg tctttattct gaaaatcaag cgaaacgaat tgaaagtttt gcttccatgg
     2341 tggctaccca tttacctcac cttacgtata aaatggcgat tgccatggcc tcagctaagc
     2401 tggagttatt gcacatcaca tacgtggaca gtattcgtgc tattttggaa gaaaagaatc
     2461 tggaagtttt aaaaattatt tgttttcata cagaagggcc agctgtatta ggtccagttg
     2521 gaaagcagat ttctctttta ggcgatctag ccgttacttc ctctggtcaa aacaatatcg
     2581 aagtgaacca taaaaacgct caaaaaggaa ttgctgttgc ccatgtcgct cacgagcgtg
     2641 ggatttcttt agaagaagtc atgacgattg gcgataactt caatgatgtc agcatgctgc
     2701 aagtagcagg agtcagtttc gcaatgggaa atgcagaaat cgaagtaaaa gattatgcca
     2761 aatatacaac ggacacaaac cttgaatctg gcgtaggaaa agccatattg cgcgcgattg
     2821 cagaagactt gtagttttat ctagaaaaga gggatataga tgtcagaatt tttcattcag
     2881 gaaaagcagc ttggacgtgc aactcggaca attgtcaaaa acgaaaaagg acattccctg
     2941 tttttgatgg ttggtcgctg gggaacaaag ggcgatgcat tgtctcttta tgccatgaat
     3001 ggcgatctcg ttgctcacat caagcagata agcctgacat tcggtacgcg ttttgagctg
     3061 tataaagagt tcgaaaaggt gggaaccatg cagaaaatat tcaattggcc cggtgacttt
     3121 tattatattc gtagacttca ctggacagtc caaggtgata tctataatca tcaatatcaa
     3181 atccatcatt tcaatcagca aatcatgaaa atggataaag caacactttt ttcaggagat
     3241 tactatgtat tggatgtctc aaatgatgaa gatgctccag tttgtatttg tattgctgcg
     3301 gttttagatt actggctcta ccgtaaagac aaaagtcgcg ataagcctgt ttcttaccga
     3361 cttaacttaa gttaaaaaga tgtacctgaa atgcggaaat acgcatttca ggtacatctt
     3421 tttagatatt cttttgcata tgatcataaa gatgaccact gatcatccgc tgaccgacaa
     3481 ctttgaagtc atggcgttcg tataattttt tagcctttgg atttttttca tccacactta
     3541 gaccgatgac ggagcgatta gctttcttcg ccaatttagg taatgcttct aataatcgcg
     3601 aaccgatgcc ttgtcctcta aaatcttctg atacactgat agagtccaaa taccattcgt
     3661 tgggaaacgt ttctttatca atgaacaaac gtgtagacgc atctagctga tatttctcta
     3721 agataggtac cagtggctga tcgatgattg attcttcttc atctgtataa ccgtaagcaa
     3781 ctcctgcaac tttaccgtct atttcttcta cgatacctcg tgaataactg taacgataat
     3841 ttggatcagt gattgcttct tctaaaatct ctaaagtctt ttgttcccct gtctgcttca
     3901 aaaatggcag ttccatatct tttagaatca ctaagatcaa tggggcgatt tctggagcat
     3961 cttcttttct cgcaaaacga atcatagttt cctctccttt tctccattct aacatgcaga
     4021 aaaagaaatc gcaaaatcgt tattttaaaa aatcttcaat cttttcagta atgaaataac
     4081 catgagtaac gattcttgcc gcttcagttc ggcaataagg gcagacgact ggcatttctt
     4141 tatcattatg ttcgactaca ttattgactt cttcgtatgc aaattttttt ccgcaattag
     4201 ggcatttcat tttttccatg ttatctcaac ttcctttcat ctttactctc ttagcttact
     4261 cttatattgt aaaaaagaga agcaatatcc ttcttctatg cagatatcca tacctgacgg
     4321 tcctgttctc catctattaa tcggacttta taataaggac gttcatcttt gactagaagt
     4381 gtccactctt tcacttttgc ttttttcccg tagtagctga ctgcttcctt catcgctgct
     4441 tgcggttgtt tgaactcttc cgttttttct atatcaaaaa gatgttctgc ggccttttcc
     4501 gtgtcatttc gtacggttgt tttcccagtg gatggattga ctgctatgac ttgtccttca
     4561 caaaaaaact ggtattcata tgttgcattc gtaggattct ccaaatcttt ttcaactgca
     4621 aaatgaatag cagatatgtt atgttctttt gtttcgtttt ttacgattgc tgctgctttt
     4681 ttcaatgaga tccgatattc ctggtcacta atatcatcca tgtgaaaagc ggcattgagc
     4741 agtcccgtca taccggtaat ccctattacg atgagggcag caacaccaaa catcattttt
     4801 ttcattcttc attctcctgt ttcttttttt taaagaatat caagaaatga acgaatgaaa
     4861 aatcgaatga aacgaaaaaa aagtgacaat tttgttatct ttattcgtgc agttctaatg
     4921 gcaatccgtc tggatcaaag aaaaacgtca tttttttcct gtaaaggtat cttctctaat
     4981 cggttctgat tcgatcccta aggactgcaa ttctgcaatt gtttcttcaa tatcgtctac
     5041 tttgaaagca agatgacgca agccacaagc ttctggatag tttggtcgtt gtggcgcatt
     5101 agcaattcca aaaatctcca attcagcact tcctagtttc agatctaatt tatagtcatc
     5161 tcgttctttg cggtaatttt ctctgattac ttcaaatcct aatttattaa cataaaaatc
     5221 acgtgatttc tgataatctg aaacaatgat ggctatatga tggatcgttg taaggttcat
     5281 atgagcactc ctattcttcg tttaagctcg gatctatatt aatctccagt tccattgtaa
     5341 atgtgtcaga ttgaaaaaaa tagctgtttt tcctttttat aagaaaaaag gcaaaaaaaa
     5401 cagaaagtag gtcctccaag acactcttcc tgttcccaat ataataaatg aaagtaagaa
     5461 attatatttt caagaaaaca aacagaaaat tatcataaca caaattattt ataattgaaa
     5521 gactagtttt ttttcaagaa aagcatacaa tagattgtgt tagtaaatac agaacacact
     5581 acatttagtg tcttaaggag tcctcataca tgaagtcata ttttcagttt ttagctcatc
     5641 agctaaaagg ctggccgcaa caaaattatt atcttttctt tttcagtctt ggctgccagg
     5701 tcatgactct tgtcaatagt cccatcactt ggctgtccgt tattacattt atcggtacca
     5761 cgcttggtgt attgtgtatt ttatccatca atgcggcaaa atcagtcaac ggtattttag
     5821 gtattttgtc tgcgatttgt tttattatcg ttgggttttc agcaaagaac tatttaagta
     5881 tcggtgaaca gattgcttat atgatcacat tggatatccc cgtattgtta agtaaagaat
     5941 ggaatcataa tatggcttca aaaatccgaa aattcactgg aaaaagctgg tttatcgcaa
     6001 tcatcagtac tttccttgtc tatcttgttt caggattcct gattggtcgt tatacagatg
     6061 atcctcggcc gtgggtagat gccattgctt tttctattag cttgactgct ggtatcatca
     6121 gtttcttgcg ttacaacaac caatattact ggtggttagc ttcagggctt gctcaaatgg
     6181 tcctttggta tatttcattt acgcatggat ctgcaagttt ggcgatgttc gtgaacagct
     6241 ctgtttactt gctgaatgat gtccttgcat ttaccgtttc accttggtat aaccaaaaag
     6301 aacgtgaacg tatgcaaaaa atggaagaag aatactacaa cgcacaacaa cacgcataaa
     6361 aaaactgagc gtgggacaaa agtaaagaac acttttgtct cacgctctaa acacgtataa
     6421 acggcggaat cagaagcaac tccttcggaa ataagctgaa attcacaaaa atttgaaaag
     6481 caattttcgt gaatttcttc ttatttcttg gagttaaacg cttctgtccc agcctctatt
     6541 ttttatagta attccttcag atactttctt gcctcatcat tgaaacgcag tgattcttct
     6601 aacttctgat aatattccaa cgcttgtttc tgagcttcag taaaacgata atgttttcct
     6661 ttccatttgt cgattgtctc ttgcacattt ccagattgga tatctttcac tactagttgc
     6721 catttttctt ttgagactgt acggtctttc ttatcagcag attgtatagc ttctcgttct
     6781 ttacgcaata cttttaaaaa accatttctg actatccgag gaatgctgtc gaagaaccct
     6841 tctgtaaaac catagcgaca atattgcttc caaataaata gatagatttt tggttggatg
     6901 aatcgttggt ttttcgcaaa atattccatc tgagcagtga cttctggtgt tcgttgacgt
     6961 tttgtctttc tcaacacgga aataaatgtt tcaaaataag cattaggatg tactggcact
     7021 tcttcatata cgatggattg ccaatcttta cgtaaaaatg cgcgatagtt gatcaagaat
     7081 tcttctacat cctttactaa ttcttctgca aagtcgtcaa atagttcttt ttttatctgt
     7141 cgcttttttg actgcccatt gatgtgttca cttattttct tgatttcatt ttccaatgct
     7201 tgataatccg ctatgtaaat cggcatttct acttggcgaa attctgcgag ccgctgagct
     7261 aattttatat ccggtaaata tggctgtttc atccgacgat tttggtacaa gacaaaacag
     7321 tatttttgcc atagtccttc aggaaaatcg atattcttct gcttaagcca taataattcg
     7381 tctaaggcca gatcaacttt tgtcattccc tgatggatcg aagctgccac tgtcggagag
     7441 acatgcaggt gatcggcaaa gtggttgatg cctagtttta ggatctttcc ggtttttggc
     7501 gatttaacga tgtattgcac ttttaattct ctaccacact cacaatacaa tttttgctgg
     7561 cttcgttttg ttcgaaaaaa cggattgatc ttgaagtcag aaaacaccca atctgttgca
     7621 gctaaataat gttcttccat aaaacgagag ctgattagat atcggcggtg cttatcgact
     7681 agttcccgct gttttttcgt taattttgca tagatctccc aacgttgact gcttgtgtat
     7741 tgtttagaaa ttttgatggg atagcttttg ctgatttctt ttggaaacca ttcttcaaag
     7801 acggattgcc attcttccac ggctgtttct ttcctttcta attacaaaaa gctggctact
     7861 aacataacgt tagcaaccag ccagtgtttc ttgctaaatt agtgagttgt tccggcattt
     7921 ccggaaatat cttctttatt ttctactgct gcttcaactg cataagtcaa agaagaagca
     7981 atgtcttcta aagacataga aggcgtaccc actggacgat cgatcacttg atcgggtgag
     8041 aatggcacgt ggatgaaccc acctcgaatt gccgggaatt gtgtttcgat caaatgaagc
     8101 aaacgataca tgatatcatt gcagacaaat gttcctgctg tataagagat gtacgctggt
     8161 agaccatgat cttgtacatt ttttgtcatt gctttgattg gcaatgaggt aaagtaagcg
     8221 gctggaccgt tttcttctaa agccgtatcg aatggctgat ttccttcatt atcagggatt
     8281 cttgcttctg ctagattgat tgcgacacgt tcgaacgaga ctgctgaccg cccacctgct
     8341 tgtccgacac agatcacgac atctggttgg tgctgttgga tggcttcttt gactacttga
     8401 ctgctttttt cgaatacggt cggtacttcc actttgatga tttcagcacc gctgatttca
     8461 tctggcattt tttttactgc ttcataggct ggattcactt tgtccccacc aaatggatca
     8521 aaacctgtta ctagtacttt catctgttgt tcctccgtcc gattgattta acatatggtt
     8581 atcttttatc ataccatttt ccttgaacag cgtaaataaa ggaaacaata gtttaaataa
     8641 cataacagat caaacttaat atattcttat aaaaaaatag tgttccacta caatgttaga
     8701 agaggttgcg acaattattc tgtcacaacc cctctcttct attatttaaa tagaatcata
     8761 taagcaattt ggaaaactag cataaataac gcaataaaca actggttttt gatcactccg
     8821 tatttgtctt tcatctccag catcgctaca ggcacgatat tgaagttagc agccatcggt
     8881 gtcagcaggg tcccacaaaa accgcaagtc aatgcaacca ttcctattaa cgctggattc
     8941 gctccatgac taaatacgaa tggtgcaccg atcccaaccg tcatcacggt gatggccgca
     9001 aatgcattcc ccatgattac cgtaaatatc accatgccaa gagcgtaaat gatgatgccg
     9061 atgttcacat ttccttctgg aacgatcgta cctactgcat tggaaatcac tgtcccaact
     9121 ccagcgcttg taaataccgc acctagagaa gctaaaagca ttggcaacat gcttaacggc
     9181 cctactgtgc ccagcatatc tgctgcatca tctaaaaatg tacttggtcg attatctcga
     9241 gaaaagaaca tcaagattag catggctgca aaaacaccga cgcccacgcc gactaaagca
     9301 cctaaattgg taaatagagc aaacaagatc gcaaagacac cgatactcaa agctggaata
     9361 aaaattttca ttcctagttt gtctgacatt ttttccatat attcttttga tggaagccgg
     9421 ctttctcctt tgctgacttt cttagcaatt gctggtatgg tcatcgcaaa gatcaagcca
     9481 ccgttcacga ttgccggaag ccatcttcct cctgcaataa ctacccctaa gaccgtccag
     9541 aaaaaggctg acccataacg atgaggattg gttttatctg ttaaatttct tactccagtg
     9601 taaatcaata cgagtcccat cacgatataa aggatctcca gtaatttatc gcctaataaa
     9661 atatcaggat ttgtaaaaaa gtccatctta ttgccccttt cctggataat atttcttcgc
     9721 taatttcttt tctttaagat agaagtagac acttgccaca actaaagccg tgatagccac
     9781 tggaacttcc actaaagcaa gctgtcccaa tgttacgtca tatcctagat ctttcagtgt
     9841 tgattggact agcaacgccc ctgctcctcc gatgaatagt acttggccaa agaaccagca
     9901 gacattttcc atagctgaag ccataccttt tagttcttct tcatgcttag gatggacttc
     9961 taaccctttt gattcgatgg tccccaatgc catcggtaaa atgattggac gaacaaatcc
    10021 agcaactcca ccaaaactca cgttaaaagc cgcaaagatc attcgaaaga tcccataaat
    10081 atctacaatc ataccagcag atactttctt gaatcgcttg atcaaggaag ccgctgattc
    10141 tttcagacca tttctttcaa gcgttcctgt tgccaacata atgatgataa aaatcgccat
    10201 cccgcgattg tttacaaaac ttgtacctaa aagttctaac attccttgaa tgccaagacc
    10261 gccagtcaaa gctgtaacaa ctaaagcaac cattacgatc aatatcgagt ctaatttaaa
    10321 tgcaaacccg agaataacaa tcaagacgcc taaaaggcta agataatttt ccatatattt
    10381 tcctccataa aatgacaata tttttttaat tataaagcaa aacacataaa aaatatagtt
    10441 gttttctcac gaaatagaca ctatttttaa atatttttcg ttattttttt atcacaaaaa
    10501 ttatcttact tattgtaagg taaagctttt tttaatttat tgcccgataa tatatagaaa
    10561 taaggtctag ttactgtttt ttttaaatga ttaataaata acaacttctt tctatgaaat
    10621 tgcaaagcat tcatcttctt ttccgctaac gatatatccg tcagtgcttg gtacacttta
    10681 ctaaaaagta tgagattgcc gatatctgga taggtgtgct atactacaga aaaagatata
    10741 caatctgcca ccgggtagga taaagaggat gatcattcca ttaggtcttt gaagggatga
    10801 ttttcctctt tttgtgtatt tttttatagt taaggaggaa tgtaagtatg gcatggatgg
    10861 aattaatcat agcaggaatt ttggaagtat tctggtcaac gatgatgaaa tggagtgatg
    10921 gtttttctaa aatcaattat tctttttaca cagtgattgg catgattgct agtttttact
    10981 ttttgtcaaa ggcaatcaaa agtttgccga tgagtctctc ttatcctatc tggacaggga
    11041 ttggcgcagt tggttcggtc ataattggtg tggtcttgtt tcatgataag ctgaatcttt
    11101 ccacttggtt ctttgtgggg atgctactca tcagtatcat tggcatcaag attactagcg
    11161 gccactagac acttggaata agtactgatc agaaaaaata agccttttta tttaatattc
    11221 aattttttta tgaaaaaata agaaataagt ccaatgacct agcgacttct ccactcatca
    11281 ggtgtatgct tgtactatct atagaaaagg agtgaatatt tttgaaaagt ctttcggtcc
    11341 aagaattatc caagaacttt ggtcacaaac aagccctcag cgatgtcagt tttagcttat
    11401 cccctggcaa aatcgttgga ttagtaggtc ctaatggcgc tggaaaaaca acgatcatga
    11461 aagctatttt aggactgatc cattactcag atggaacaat ctgtattggt gaaaatgaga
    11521 tttctccctc tagtcataaa gggttggaac aagtaggagc attgatcgaa tacccgggta
    11581 tctatccatt cttaacaggc tatgatcatc tgaagttatt ctctgaaacc aacgatgtat
    11641 ctgctatcga cactatcgtg aatcagctca agatggaaaa gtacattcac aaaaaagcga
    11701 aatcctactc gttgggaatg aaacaaaaat tgggaattgc tcttgcctta ctgaaccatc
    11761 cgtctatcgt tattttagat gagccgatga acggtttgga tccacaagca acaagagatg
    11821 tgcgaaaaat cattaccgac ttggctactc agggcacctc tttcttgatc tccagtcact
    11881 tactcagcga gcttgaaaaa atggtcgatg agctgatcct gatcaatcaa ggaaagatca
    11941 tcaaacaatg tacaatggct gaattgaacc aaacagcgca agatttcatc gttatctcta
    12001 caacaaatga tctagctgct cgaacgatct tgacagaagc tggatatact ttggaagatc
    12061 ctgataaaat caagctttta aaagaaaacg aagaattatt aggaaccgtg atccagctat
    12121 tgtctgcaca aaatatccat gtgacagacg tccagcatct gacgaatgat ttggaatctt
    12181 ctctattgga aattctggat tcagtaaagg gggaagaaga aaaatgacat tgcttgtaaa
    12241 acaagaatta tttaaattga tcaagaaaaa gtcaactgct gttttatctg ttctattagt
    12301 tgttttgctc ataggaacag cacttctagc caaaaaatac acgacgatca ttgatccagt
    12361 tgaaatgact gctcagcttt ttagtgccac atcttggatt gtctttatca tgattgcagc
    12421 tgcaagcacg atcatttcga tggaagcaca atatggtaca ttgaaaaatc tcctttatcg
    12481 aaagtattct cgaggagaaa tattagtaag caaatggatc acattagtta tttattctgt
    12541 ttatttatac cttttagcta ttattgtcac tgtgttgatg aaactgattc ttttcccttc
    12601 gatttccttc acggagaagg tttctactgg tcaaacactg atccaaagct tgttcactta
    12661 tactttgggt agttatatcg gtttatggct gattttaagt ttagtgttga tgattgcttg
    12721 ctttatcaat agttccggcg catccatctc agcaggaatc gtcttttatt ttgcttcgtc
    12781 aatcatatct ggtatcctta ttgcactcat ccaaaaatgg gagtggatca aatggaatcc
    12841 gatcagtatg ttgaatctgc aaaaccaaat tggaaacgaa gagatcatga agcaattgac
    12901 tcatctttct actaatcaaa tgctattcgg taatttagct tacatcgttt tgttcttagc
    12961 attaggatat ctcgtattta agcgaaaaaa tgtatgatga ctcatcaaaa aagaggctgt
    13021 gaaagagacg ttcagcctta ttgccgattg gctgtctctt aaacaccgtt tattctgaaa
    13081 aaggagctgt gacaacattt gtgtcacagc tcctttccta gtatgttttt gaataaacga
    13141 caggctcaca acctctcttt atacttcttt tatacttcta tacgtgaagc atcccagtgg
    13201 caaataaaac cattgcaatc actccgacca tcgtcacacc tataagtaaa atcttatcta
    13261 agtggatatg ttgtacaggc tcgttttgct ccttacgtgc tttgaggtaa aaataaatgc
    13321 ctggcaagaa aagaacaaga acaagcatta cttcattaaa ggctacgact agcatagccc
    13381 atacttgaaa ggcacttgct aaaagcccta ctaaaaattg gacccattct ttccgctgat
    13441 aagaaagttt catttggtac aatcctaaga aaagccacgt gaagaaaata gctgctgtac
    13501 caagtgtgta agcaaagtta taagcactgt cagtgaataa taatgaaagt aaaaagacat
    13561 tcgtacataa cgtcgtcaat acgatagcat aagttggtgc cccatttttg ttgagttttc
    13621 cccatgcttt aggcaaaagt ccgtcttcag ccatgtttcc cattgtttca atcggtaaga
    13681 tcgtccatga gagccaaaca ccaactaaag aaatgatcaa accgccgttg atcacgactg
    13741 ctccccattt accaaccatc atttcaaaca catagcccat agcaggctgc gccttgatcg
    13801 tttgcagctg attcatcgtc attgtcccat atggaagcat ggaaaccaag acgtaaataa
    13861 taatcaagcc gaatatccct agaatcgttg ctttcccaac atctgattgt ttttttgctc
    13921 gtgaacctac gacagaggct ccttcaatac caacaaatac ccaaagcatc accatgatgc
    13981 aactcaatac ttgactggaa aacgggatcg gctgtccatt cgcattcaat ataatgtcgc
    14041 tagaaaagtt atcaatgaat tgttggatcg aaaacgtgct gatagctact acaataaata
    14101 aaaataatgg cactaattta cagaatgtga cgatagcatt gacgaaacta gcattttcga
    14161 ctccccgatt cactagccat gcatatcccc ataaaaagac tgtactcatc acgatcgatg
    14221 ccagattttg cccaccttca aatactggga agaaatatcc taatgcgctc atcaacaatg
    14281 ttgcaaaagc tacgttgcct aaccagcatg tgagccagta accccaaccg ctgataaaac
    14341 cagcgaaacg gccaaaacct tcttctgcgt aactaaagat cccgcctttt aaatctggac
    14401 gttttttatt gatattattg atcgacaagg agagcatcaa cattgctgtt cctacgagta
    14461 cccaactgaa aatagctggg ccgccagccg atccggagat gtccgtcata ataccaaata
    14521 cgccacctcc aatcgctgaa ccaattacta aggcagcaag tggaattaaa ccaattcctt
    14581 ttttctgttg ctctgtatcc atgtaagcat cctccaaaat aaaaccgctt ttatcaaaaa
    14641 gtataacaaa ccttttttat ttttacttaa ttaataaaaa ctttaataga aatataattc
    14701 taccggcttt tttctcatta tacaagcaaa gcgcctagtc cctttccaaa ttacaccacc
    14761 aaaaaactta aaaaagatgc tatactaaaa ggaaagaatt ggaggatgat ccatgaacat
    14821 tttatatata tcgatttcag gaaatacgcg tgcctttgcc aaacatctag cagaatatgc
    14881 tgaaaaaatg catgcagaag atcctgtcaa tcctgaagtt actttaaaag aaatccatga
    14941 aaatagtgat tttacgaaag aaacagaacc attttttact ttcgttccta cttatttaga
    15001 cggcggaaat ggtttagata acggtgatac agaaatcttg accgaaacga tgcgtgaata
    15061 tctggaacat gaagacaatc ataaactttg tcttggtgta gtcggtagcg gaaataaaaa
    15121 cttcaataat cagtattgtc taacagccaa acaatatgca caaacatttg gctttccttt
    15181 cttggcagat tatgaattac ggggaacacc atctgatgtg acacgaattt atcagatttt
    15241 agcagaaaac aataaataat aaaaaagaag aagctgaaaa atagatttca gtttcttctt
    15301 tcgtttctat tgtttctatc atattaagct ttgacatcca tcacaggtgc ttttgctttt
    15361 gctttacctg ttttttccaa gacaaagttt tttacacgat ccatattggt taccaagacg
    15421 atcatggatg tttctttgcc tgctgctttg atcgcttcta agtcagcttc tgctacaagc
    15481 gtatcaggtg tcacttgctg cccttctgtt actttcacat caaatggcgt accgcctaag
    15541 tcaactgtgt tgattcccat atgaagcaag atttctaaac catttggcgt tgtgatccca
    15601 atagcgtgtt tcgttgggaa gacagagctg actaccccag taactggaga atagatatgt
    15661 ccgttttctg gttctacaaa gtagccttca cccatcatct tttgtgcaaa aactggatca
    15721 cttgctttat ctaaagaaac aactgttccg tcagcaactg atgagaggat agtcacactt
    15781 tcagctgaag tttgaggtac tactttttca ggcacttcat ttactgtggc tgtttcagtc
    15841 gctaattctt ctttttctaa acggtcttta ggaataccga agaagaatgt agcaacgaag
    15901 ccaccagcat aagctgccaa taatccaagg atgtagccta accatttacc gtcagagatc
    15961 aatgggatca aagcggctcc tgaaggtcca atagcgatag ccccgacatt tccgatcatt
    16021 ccgatcactg ccccgccgat acctccaccg atacaagcag taataaatgg gcgtcctaga
    16081 ggtaatgtta ctccgtaaat caatggttcg ccgattccta ggatcccgac tggtaaagct
    16141 cctttgatca tttctgttaa ttctttatct tttttgcagc gaatccataa tgccaatgct
    16201 gcacccactt gtcctgcacc agccatcgca agaattggaa gcaatagcgt acgaccagtt
    16261 tcatcaatca tttgcacatg gattggagtc aaaatttgat gcagtccgaa cataaccatc
    16321 ggcaagaata atgtgcctag aacgaagccg gcaaatgctc caccaacgcc aagtacccag
    16381 ttgatcccac cgaccatgcc atcagaaatg aaacctgcta aaggcatgat gaagaaaatt
    16441 tcaatcagac cgatggcaat caatgaaaga gttggtgtaa cgatgatatc taccgcatca
    16501 ggtactactt tatgcagttt tttctcaacg attgacaata gccaaacagc gaagatgaca
    16561 cctaggatcc caccttgtcc agctgcaaga gcttcaccag taaataagtt tttcagtggt
    16621 gcttccggat tcatccctgt cagcatcaca acggccccga taactccacc aagagttggt
    16681 gttgcaccaa aaacttgtgc agcattgata cctgtataga taactaggta gctaaacatc
    16741 ccgtttttca agatgttcat cacatcgata tattgttgcc atgttgctgc atcaagtgtt
    16801 ccggctgtga ctaagtttgc taaaactgcg gcaatccctg ccacgatccc agtaccgaca
    16861 aatgcaggaa tcattggcac aaagatatta gaaatgtctt ttaataccga tttgatttta
    16921 gatggttttt gtttcgattt ttgtgccgct ttcatttctt gtgcccgttc gtttacacgg
    16981 tcttttccag atgctgatga atttgctgta gctgtactta cagaagaaag tttttctcct
    17041 aatttcacgc ctgccaaatc aaccatttgc tgtgctactt tgttcacttt cccaggtccg
    17101 atgatgactt gaagctgttc atcttctact acaccaagca ctcctggaat cgcttttaat
    17161 tcagagacat ttacaagatc atcattgatg atcttcatac gcacacgagt catgcagtgg
    17221 acaatgcttt ctacattgcc catgccgcct gcccctttat aaatttgttt tgccagttct
    17281 tccaaagtca gttcgttttt tgccatggtc ttttccccct cataaggtcc cttttacaaa
    17341 tccgtttgct tcagctaatt ttgcttgagc ttcctcttta tcactatttg tcaaaatcat
    17401 gacgatagcc agcttaacat tttgatctgc ttcttcaaag tacttttcag ctgtttcgta
    17461 atcagcttct gtcgcttgca tgatgattcg ttttgagcgt tcgattagtt tttcatttgt
    17521 tggtttgaca tcgaccatca agttattgaa tactttacct ataccaatca tactgatagt
    17581 agaaatcata ttcaagataa gtttttgtgc tgttcctgac ttcaatcgag tagagcctgt
    17641 caaaaattct ggacctgcat cgacttcgat tgggaaatcc gcgtgtttac tgatttcggc
    17701 ttgtttattg caagaaatcg ttcctgtgac agcaccgatc gattttgcgt aatctaatcc
    17761 accgatcaca tatggtgtac gaccgctagc tgcgatacca ataactatat cattttttgt
    17821 caaattcagc ttgattagat cttcttgccc tagctcataa gaatcttcgg ccccttcgac
    17881 agctacagtc attgcttctt ctccaccagc aattagacct atgaccattt ccggttctac
    17941 accgaatgtc ggtacgcatt cagcagcatc taacacacct agtcttccac ttgttccggc
    18001 tcccatataa atcagacgtc cacctttttt aaaggactca atcgtgttag caatcactgg
    18061 ttcgatactt gtcagttctt tttcaaccgc agcggccact ttctgatctt ctttgttcat
    18121 tagttgaacg gcttgagaaa tactcatctc atctaatcca aaagtttctt ggtttctgcg
    18181 ttccgttgtt aggttctcta aattgatttt gctcattttt atcttccttc ttttcttata
    18241 aattgatagt tcataccagc tttgatttgg ttgatcaatg gtagctcttc tttgataact
    18301 ttagctacta cattcacttt ttcatccgct ggcaaatcat atttggttaa ttggatctca
    18361 cccatatagc gcaaatactt ttcgttatct aaagtaatag accctttcat acgcgtagct
    18421 gtatttcttg ctgggatagg cggaatctct cgaaaacgtg catctacgct tcgtatgaca
    18481 tctcttgcat cgtcttgccg attcgtatgc ttccctaata tatattcgta aaattcttca
    18541 tctacaggtt ccgtatggag caagactgtc tgttcttttt ggaaagaagc aaattgctcc
    18601 tgaacgtttt ctgacagacc tgcatctccg atgtaaacaa tatccgtgtt acttgctaat
    18661 agatctagag cgcctgcaag aggatggatc cctcgatgtt cttccaaagt tggcaatcct
    18721 tgatatagtg ggcctctcaa ctctgtatct cctggtacaa aaccttggac aaggagacct
    18781 tgaattttga gccattgatt ttttttctga taccattctt tgtccagccc tgtttcggga
    18841 cgtggataat aattatgcca agcttcaatc tgtgagaaat cagcttctgc ttctttcaat
    18901 tcatcgatat ctttaggcgt gatcgtgcta gcattcaaac tgattttcaa ttgatgtgac
    18961 cagtcggcaa tctgctgatt ggaaatatga taatccattc ttaaacctgt tacaccaatt
    19021 gctttcaaag gcgttagatc ttctaatgaa aaacccgctc gttccaatgc ctctcctgaa
    19081 atatcgacca tcaattctag tttattcttt ttcgcttcgc ttcctagttc gatcaatcgc
    19141 ttttgatatg ccgaaacatc atcttctggt atatgcatgg aagtgaaaat accgacaaag
    19201 ccattggatg ccatttggcg gatatactgt ttcttctctt ctgacaaaga atcgttcata
    19261 aatattgaaa agccaaacat attatccctc tttttctgta tccgtcttca tttctatgac
    19321 tagagtatat atctaaagaa acaaaatttc aatactttta ttaaaataat taaatttcat
    19381 ttctaaaggt tgagtttata ttatcaaaag aattgtcata acctaaaaaa ggcactgcta
    19441 agcaaaagca gtgccttgaa atattttatt taaataacac aaaaacataa ttactcataa
    19501 gaaaccaatt ctgtattatc taccatgaat gtaaagaacc cctcattagg catcggatga
    19561 aaataagcat tgatcactct tatcaaggca ttatggcaaa ctaacagata gacattttct
    19621 tcgtcttcga tgcattcttt caataaagga acgatccgtg catacacatc taagactgac
    19681 tccccatttg ggaatcgtac agcgaacgct aaacgtgctt tttgaaagtt ctcatctttg
    19741 cttggcagtc catcataatc cccaaaatcc atttctacca atcgttcatc aacggtcagc
    19801 ggcaagctga gacgatcagc aactgcttgt gctgtatctc ttgctcgttg aagaggggaa
    19861 tggatgattt tcgtgattgg aacaggcaaa tctactacct tttctgctag tttctgtgcc
    19921 tgcatctgtc ctttttcagt cagcggcaca tctgcccgtc cgcaaactaa tccttgtgcg
    19981 ttccaagttg tttctccgtg tctcgttaca taaagcatgt gattctctcc ttcgttggca
    20041 aagttcccct taaccatacc ttgttgttct tgagattaca agggttagat tctctcaaga
    20101 gaaataatct cagtagatac tttatccagg aaggtttgat catgttccac tagtaacatg
    20161 gcaggtttca cgttcaagat cagttgttct aattgttctt gattgaagac atccaaataa
    20221 ttcaatggtt catcccatgt atatagttca gctggctgtg ataaagattt agccaattcc
    20281 acttttttcc gttggcccat actcatctgc tcgatcttgt tatgaaaaac atctctttcc
    20341 atcccaagct ttcggaggtt gttcaaaaat gcttggtagt ctacttggtt tttctctgca
    20401 aattccgcca acgttcctcg attgtcttca taattttggc ttgcataact aatgctcaga
    20461 tgttttggct ggtatttttc tcctataacc ttgccgttga atgcccccag aagataatgg
    20521 ataatggatg actttcctgc accgttagga cctgaaatcg ccacccgctg atgaggctcg
    20581 attgtaaaat gaattggctc gaataacaga ttttcatacc ctaattgaag atcttctacg
    20641 cttaaaagtc gcttatggtg agacgcttgg ctattcatcg tcaaagaatc gatatattcg
    20701 atatctttta atagtttttc tttctcgctg atctgggtct ccatccgatt cacgattgct
    20761 ttggatcgtt tcatcgtccg tgcagcatca gcacccactg ctcctttatt cactcgtcta
    20821 gattcagtat cgatgaatcc gacttgtttc tttgttttat ctccttctcg ggaacgagac
    20881 cattcggctt tttcagctgc tgttttcttt agcctactga cttctttttt caatttttca
    20941 ttttgagcca tttcaaattc atcacgaagt tttttctgtt cttcatagat agagaaattc
    21001 ccttgataaa gttccagttg acttttttcg attgctaaaa catggtccac tacttcatcg
    21061 ataaatcccc ggtcatggct gaccacgatg aagccttgtt ttttcttttt caaataagcc
    21121 gctacttgtt ttctaccaga gatatccaaa tgattcgttg gttcatcgat taacgggaaa
    21181 tgagtgtcat ccacaaataa aagtgccagt aggacttttg tcttctctcc ccccgatagt
    21241 gtgctgaatt ctctccataa gatttcagga tctgtttgca tcaattggag ctctctttcg
    21301 atttcccata tctcaaaatc cgtaatatca tttaacacgt aataggttaa acgttcttta
    21361 tcttttgtct gttgcgggaa ataggcaaat tcttgctgat ggattacttg cccttgataa
    21421 ggtagtttgt tttgtagaat attcagtaaa gttgtctttc ctcgaccgtt tcgtccgata
    21481 agtcctagtt tccattgtgt gtcaaaattt agatttgctt gttcaaataa taatgtgcct
    21541 tggctgtcgt agccgaatgt cagatttttt atttcgattt tagacatttt ccatcacctc
    21601 gtaaaaaaag accagtccta ttttcagaac tggctaaaga ggatgcaagg gttggataca
    21661 cacaaaaaga cccatacatc gaaaaaacca agccaatccc gaaaatctgc acactgcttc
    21721 cttgtctcca caaggtacag tctgaatgca gctttcatca atcgacttaa tttttcaatg
    21781 cacggatccc tcactttgtc ctatatttga aattacgata gcataaagaa aatgattttg
    21841 tcaactactt cgggaggatg gtgaaaaatc tgctaagtgt ctgtcctaag atttttgttt
    21901 tttctaatgt ttgcctgtgt tccattgctt cttctaaacc tattggaccc aaatgaatac
    21961 ttaagacaat tgggagaaaa tcagaaagtc cttctagctc ttggtctcta cttccgcaaa
    22021 tagcgattat tggttttcga tatttttttg caagcactgc tacaccgtat ggaacttttc
    22081 cgtggatcgt ttgctgatcg atccgccctt ctcctgtaat gatgacatct gcttgttcga
    22141 tctttttttc caatccagtg atttggctga ctaaatcgaa accagaaacc atcttcgctc
    22201 ccaacaaaaa aagggctcca ccaataccgc ctgctgctcc acttcctgga atatcatcta
    22261 aatccacctc ccagaaatct tttatttgct gagcaatctt tcctgcttgt ttatctaaga
    22321 aagctagcgt cgtatctatt cctccttttt gtcttccaaa ttgccaagca gctccttgtt
    22381 gacctgtata tggattggta acatctgcag cgataatcaa ttttacttta gaaacaagaa
    22441 cgtttcttcg atcaaaatga agttttttag tagataataa aggatttcct tctgtgtacc
    22501 cttctaaaga agcacccaag gattggagta atcctagacc accatcgcta cacccgcttc
    22561 ctcctaaagt gacaataatt tgttctactt cttgaagcag tgcatcttta ataagttctc
    22621 ctaaggcata actggaagca agtcggatag tttgatccgt tggtttatca agtagatcta
    22681 accccaatgc ttgtgcagat tcgatgactg cggtgttttt tccttgccaa gttgtcaata
    22741 aatagctgat ttttcgctga cggaataaga gatccatccc atcaatggta cgccatgttc
    22801 ccctgagcgc gtaatagatt gcttctgcag tattttctcc gccatctgca atcggcacat
    22861 tgatttttct tcctttacga tcttcccagc catttagaac tgcctgattt agttcaaatg
    22921 aagaagcact tcctttaaat gaatcaattg ccgccaaaat atccatctgt atccctcact
    22981 ttcttcttaa gtaagacact ttttttcatt tttttcaaac aaaacaatgc tattttcttc
    23041 catttttcta tatactaaaa gggaactcat tgaataaagg agagaaaaaa catgaaaatc
    23101 gcaatagctg gggccggagc aatgggcagc cgaatcggat tgatgctaca tcaaagcgga
    23161 aatgaggtcc tcttgatcga tcgttggcct gctcacattg aagccattcg tacaaatggg
    23221 ttgattgctg actttaacgg taaagaagtg gtcgcaaaac tgccaatcta ttcaccagaa
    23281 gaaatcatcg aaagcaacga acacgtggac ttgatcgtag cattaacaaa agccaatcaa
    23341 ttagatgata tgttttgttc gatccaatcc attattactg acaatactta tgttttgtgt
    23401 ttactaaatg gattaggtca tgaagatgta ctggaaaaat atgtgccaaa gaaaaatatc
    23461 ttatttggga tcacgatgtg gacagctggt ctagctggtc ccggaaaagt cacattgtta
    23521 ggtgatggag aaatcgaact agagaacttg gaaccagaag gcgaagcatt tacaaaaaaa
    23581 gttgtagaag tctttcaaga agcaaattta aatccgatct atagccataa cgttcgttat
    23641 tccatttggc gaaaagcttg tgtaaatgga acattgaatg gtttatgcac gattttggat
    23701 tgtaatattg ctgaactagg cgcgcaaaaa gcggcagaga gcatggttcg aacaatcgta
    23761 tctgaatttg cttctatcgc agcaaaagaa ggaatcatac tagatcaaga agaagtgtac
    23821 caacatattg cttctactta tgatcctgac aacatcgggt tgcattaccc atctatgtac
    23881 caagacctga tcaaaaacca tcgtttgact gaaatcgact acatcaatgg tgccatttgg
    23941 cgaaaaggac aaaaatatga tattgctaca ccatattgtg cctttttgac ccaattagtc
    24001 catgcaaaag aaggaatcct tggcgcagaa taaaaggctc tctgatgaga atcagctgaa
    24061 tcagcagctg gttctctttt tttaagccca ataaaagatc aaaaagaaaa cacaactctc
    24121 atgatgccat aagaattgtg cttgtttgtt tcagtacata atctcttcaa aatgtaggtt
    24181 ttttatttgt tttcaacaga ggagaggtca tattcgtatt gctcataaat agtggaatcg
    24241 ttagtgataa atcgttttgc ccaattttct gcatcataaa attccaatag ctctgatagg
    24301 attggcttaa atggtgtatg cagaaattcc ggtaattcat ctaaatcacg ttctcgaagc
    24361 aaaaaaatat tttcaggacg ataaaaatga taatgagcaa tcgcttgatt atccgtcact
    24421 agtttctttt gaaaatacat ggattcaaat gtccgcaaag tcactccttg ctgaccctcc
    24481 cgcaacagtt ccagaatccc gcgcgattcc cctgtttttt tcaggtactc ttcataagta
    24541 agatatccag gttgttcatt ccctcgatgt ggaagaatga aatagttggt cgtaacatct
    24601 ctttttataa attcctgctt atagctttct gctcgttctt ttctcccatt attcgttgca
    24661 ccaaaaaata gatccgtctt tggttcgcta gaagaaaaat tcatatgttt cgaataaaat
    24721 gaactattat gtttcagccc aagttctttt gcttccaaaa aatcaaaatg ataaatctca
    24781 tcgattgccg gttcatcttg tgcctgcttc agcatcgcct cgtgttctgc tttgaagtga
    24841 ttccaaaaaa ataacacaat ttttgcattg gtaaatgggc gcacgcgtcg agccatctcc
    24901 atatcaaatc cataatctaa aaaaacgatc agctgataat ttgccaactg ttgtctagct
    24961 gatttaatga attccgactg tagtaaatct tttccggacc aaacatctaa agagcggatg
    25021 gaaggcaaaa aataaagtga tttatttttt gcaactagca gtacgtcatt tttgttcatc
    25081 ttttccgcct cactttctct gtcacttttt cctatgatag cctgtttaaa tagtaaaaag
    25141 ccagtaaaac gtttcctata tttgtatgaa aatttaacat ttgtatgaaa atatgatgaa
    25201 ataaatcatt cttctattaa aattcgccta aaagttttct gaatagttgt aattgtctga
    25261 aaatttgtta tcattagtca ttgttaaata ttgctattta ttcttaatat aaattttgat
    25321 tgtgaattta aaagaaagag gtcatttatg gaagataaaa aattgaaaaa acaacttagc
    25381 tccagacata tcaccatgtt agctttaggt ggagcaatcg gtgcaggtct atttaaagga
    25441 agtggcgaag cgattgggat cgccggccct tctgtattga ttgctttttt gattggtggg
    25501 ctgattttat atatcgtgat gaaaggtctg ggaaaaatcg ttttaagcgg tggcgacacg
    25561 catcacggat tatctggttt gatacgccct tatctaggtg ctcattctgc tgattttact
    25621 gattgggtct actggtctat gtggattatc aatatcatcg ctgaagctgt tgcagctgct
    25681 tcctttttgc agttgtggtt cccgcacatt ccagcctggg tatttgtttt tatgttggct
    25741 gttttaacta cgatcatcaa tctttattcg gtacggttat tcgcagaaac agaatattgg
    25801 ctggcttttg ctaaaatcag tgtcattatc ttactgatca tttttgctac ttatcttgtc
    25861 ggtcagcaaa tgttgggcac aggcgttttc cctacccttc agcaattgac tgaccatggt
    25921 gggtttactc ctcatggtat gaagggaatc atcagttctt tgcttgtagt tatctattct
    25981 tacggcggtt ctgaactgat tgctatcacg gtaagcgaag cagatgatcc taaacgtgcg
    26041 attcctaaag caatcaaagg tgtcatggga agaatcattt ccttttacat cattcctttg
    26101 tttttgcttt tgattctttt tccttggaat actttagcag gcacgaatgt cagtcctttt
    26161 gtgatggtat ttgaaaaaat gaatatccca tttgcagcgg atatcgtcaa ttttgtgatt
    26221 gtccttgctt tgttctcatc tatcaattcg ggtgtgtatg catcttcaag aattttgttc
    26281 ttccgtttga aggatcgaaa aggttcctct agaaaactag ctgttttaaa caagcatcaa
    26341 gtaccgcaac gtgctgtttt attttgtacc agtgttcttt atcttggtgt agctctttct
    26401 tattttgtcg gtgacaaatt atttggctat ctggcaggtt ccctttcgta tacagtatta
    26461 ttgatctgga tcattatcag tgcggcatcc tttgtactgg cactaagaaa aggaacatta
    26521 tgggataaag gaatcagctt ttttgcatta gctgtcttag gattgatttt ccttggtatc
    26581 ctgttcacga actccattgg ggtaactgta ttgactggcc tgctttacct gttcatttat
    26641 ttcagttatc aaaaaaagaa tgatgcgttc gtattgacga atgaatgaaa tcgatagaaa
    26701 attttatttt atttatgtgg aatagatgga gctattgaag aaaggagttg tgacaaaagt
    26761 cttgttgcaa ctcctttttc cgaataaaca gtctcatgac ctcttggtaa acggtgttca
    26821 aaaaccctct gtgcaaaata attgattgtt ctccatgctc atgacatgat agaagaaagg
    26881 aatgaaaaaa aggagtttgt tttaaatgaa aaagtattgg aagtttttgt tgatgatcgg
    26941 tgtttcaacg gtagttatgt ttggtatgat gtacttgaat gtctatgcgc tcgatcatat
    27001 cttcttcagt cgtacacgag tattcatggc gttgatgatg ggtgcagtca tggcagtcat
    27061 catgctgttg ttcatgtgga aaatgtatga aaacaaaaag ataaatgtta tgatcttagc
    27121 cgctagtctt cttgtttttg ccggttcctt gtttatggta cgaagccaaa ctactgtcaa
    27181 cgacacagct tggatgaagg ccatgattcc tcatcattct atcgctatcc tggcaagtaa
    27241 acgagctgat ttaaaagatc ctgaagtaaa aaaattagcg gaagaaatca ttcaggcaca
    27301 agaagaagag atcgacaaaa tgaaaaaaat gatcaaagaa atggaatgac cattatccgt
    27361 ccaaataaaa aaagaggatg ttccagaagt tatcagcttc aagaaataag aagaagctac
    27421 ttctggaacg ccgtgaataa ggggttgtga aaaagctgcc ctgcatcaag taataagaac
    27481 agccaaacaa aaatagcttc tcctattttt cgtttggtta ggcttattac cgcagatgca
    27541 agcttttgaa caccgtttat taagaggttg taaaatcagc gttctgacct gagtattaga
    27601 gagacaaatg gaaaaatagt tcctcatatt ttgccatttt ttgagctaat accgagggtc
    27661 agctgattga acaccgttta tcgagaggtt gtgaaaaagt cgcttagttt cgcgtattaa
    27721 gaaaaatttt gtgaaatggc tttccacatt tttgtataaa tttggcttaa taccgagaaa
    27781 ctggcttttg aataccgttt attcgtaaaa gggattgtga caaaagtctt gtcacaatcc
    27841 ctttttatgt ttttattgtt ctaattgcgc tgtcttgctt actgcttctt gcggtttcac
    27901 aaattttttt gagattcccc aacctgtgaa ggcactgacc atcgaaaaca caggagaaag
    27961 caaactgaaa aatacgaatg gcaagtaagt gagtgtagga ataccaaatg tacttgctac
    28021 gaaacttccg gcaattcccc aaggcactaa gtagttgatt actgttccac catcttctag
    28081 cgttctacct aatacagttg gatcaaatcc tgctttttca taagtttctt taaatgcatt
    28141 accaggcaga ataacagata ggaattgttc accaataaaa atatttactc cgatacttgt
    28201 catcaatgtt gtaaaaatca aatttggtgt gttcgtcatc tttcttgata ctcgttccat
    28261 cacggttgag atcaagccaa attcgatcaa caatccacct aatgacaaag ttagaataat
    28321 caatgacaca gtaggcatca tactttcgat tcctccgcgg ctaagcagtg aatcgatttg
    28381 ttggtttcca gtatttgaaa caaaccctga ttcgatgacc ccagccaact tcgttaagga
    28441 agtttgtggg ttttgaataa aaatcattcc tactgctaca gcgatattca acaaaatcgt
    28501 taaaattgcc ggcacctttt tccatgcaca cagtaacatt aaaaataaag gaatcagtga
    28561 ccagattgaa atagtaaagt ttgcttctag aacatcagtc acttcacgaa tagatgaaag
    28621 acttgcggct ttacttgtgt gtcctaaaat catgaataaa aagaaagaaa caacaaatgc
    28681 cgggacagtt gaccacatca tatgcttgat gtgtttgaac aaatcggcat caacaatagc
    28741 tgctgctaga tttgttgatt cggataatgg agacatctta tctccaaaaa cggcacctga
    28801 aacaatagct ccaacaacca gcgctggatt taagcctagt gttgtcccaa taccgaaaaa
    28861 tgcaatccca atcgttgaca taacagtaaa ggcactccct acagcgctac ctacgattgc
    28921 acacacaaca aatacagaag ggacaaacca ctggacactg atcaatttga acccaatgac
    28981 cattagagtt ggaatgatcc ctgcttgtat ccatacagca atcaatgaac cgactaaaat
    29041 aaaaataaag attgggatga ttcctggttt gatgccattg atgattcctt cgtgcagcca
    29101 atctgtcggg tatcttttta tcactgcata caacatgacc attccgatcg ctgctaagat
    29161 cgtgatatta ggtgagacct ttaatccgac aacgccaatc gcaatgccta aaacaataac
    29221 cattaataca agtgttgctt ctaaaaaact tggtgttcct ttttttccca tgataatctc
    29281 tcctttttta gttgttctta tgttctaaaa aagttaagat atcggattac tccgccgcaa
    29341 ttcatccgcg attcctcctt tataaaaata aaaagacctg ttttaaacta aggacgaatg
    29401 actccgcggt accaccttaa ttaaaaacag atctgttttt cactttcaaa cttttattca
    29461 aatgtatgga ctttccatgt aattcgtcaa ttaggcttga tcggttcaca gcgaccaccg
    29521 actttctgaa caaaacacct aaccgctact gcgtgaaaag ttgacttgta cgaagtttac
    29581 cttccataag atcatttgtc aatgaaaaag aaaatatttt ttcatttttc tgaaaattat
    29641 ttttataact ttcacacaca agtttatttt ttactagaaa gtagcgccct cttgtttttt
    29701 tcttgctact atttcttgat gttcttgctt gattctttct aatagctttg gctctttcaa
    29761 cagatcaaga gctgtattgg cgagtagttc tgctgcaatc gcaatagaag caaggccttt
    29821 gtcactttta gcagcagctt tgaattcttc tgtgtgtccg gcaatatatg tctcagagat
    29881 tgctaccgta ggctggattg ttggtatgac ttggctgaca tttcctacat cagaagaacc
    29941 gagacttgtt ctttgttccg tttctatttc ttcatctgga acacctgctt cttttgcatg
    30001 ctggaagaat aagtcatcaa aagaaggagt aacgatgaca tcatcaactg cattttggaa
    30061 caatccaaac tcaaaagtcg tgcccgtact tatcgcagca cctcgtacga tattttccac
    30121 tttttcatat actttatcta atgtttgccg atttcctgca cgtaaataga aacgtccagc
    30181 agcgtaatct ggaatgacgt tggcagctac tcctccgtct gtaatgatcc catggatatt
    30241 gacatctttt ggcaattgca agcgtagagc gttgattcca ttaaacactt ggatcaatgc
    30301 ttccaaagca tttactcctt tttcaggtgc tgctgctgca tgggaagcga caccgtaaaa
    30361 tttgatgtcg actggatcat tcgctaaaga ttctgtcgtt ttcccataac gaaaagctgg
    30421 atggacgcat aacgctacat cgacatcctc aaaaaaacct tctcgaacaa agctgccttt
    30481 agctgatccg ttctcgcccc cttcttcccc tggggtgcca tatacacgga tttctccacc
    30541 agtttcatca atgaattgct tcactatgct ggcggctaat acggagtaag taccaaacaa
    30601 attatgcccg catgcatgac caatacctgg taaagcatcg aattctgcca aaaaggcaat
    30661 tgtgggaccg ggtttattgg aaatataacg tgcatcaaag gctgttcggt gtccagctac
    30721 ttctttttct acccaaaacc tttctttttc taattgttta atcaacgctg ctgaggaata
    30781 aaactcatag ttacttactt caggatgttc gtggatatct agagcaagtg cttgatattc
    30841 tggtaaacga tattggatta gatccttcaa ttggtttgtt gagattgttg ttgtcataaa
    30901 aaattcctcc tatgctagtc atttttctta ttttctcccg aatgtttccc atgcaggcac
    30961 gcttgatcct tttgatgttt tctcgattac tttgactgtg tcttctgttt gataagcgtc
    31021 tactattttt tgatacgttt tattgttttc atcttctttt cttgcaacga tgatattcac
    31081 ataaggacgt gcatggtcat ccactggttc taagaagatg gcatcagaag caggattaaa
    31141 tccagcatct acagctacac cgctattgat cactgaaatg tctacatctg acaaagcacg
    31201 tgctgtttgg gaagcatcta attcagaaat gtctagattt tttctattct cggtaatatc
    31261 agaaacagtg ggcgcttgtc cttttgcctt atccactttg atcaatccag cagtttgcaa
    31321 taacagaaga gctctgccgc catttgtaac atcattaggg attgctactt ttgctccgtc
    31381 tttgatatca gctacatcct tcaaagtgtt agaataaatt cccagcggtg cattgacagt
    31441 atttccgatc gatactaaat cagttccgtg ttctttattg taattgtcta aaaagaattg
    31501 atgttggaaa gaattcaaat caatcgatcc atctgctaaa gccacatttg gttgattgta
    31561 atctgtaaat ttcacgtatt ccaaattaat gttctccttt ttcaaacggt ctttcacgtt
    31621 gtcccataca tctgtatctt ctccaataat ccctagtttt acgatagttt cttttttgtc
    31681 agttgtatct gcttttcctt ggctacaccc tgccaaccaa ataattgcta caaatgtaat
    31741 gactacaaaa cgaataattt ttttcatttt ttctcctcca ctttcaatgt gtaatttttt
    31801 taatcagttg attactgata aattgactta aaaaaactaa gattagaatc aataatgtag
    31861 ccacaatcgt tacatctgtt tggaatcgat tataccctct cgatatagca agattcccta
    31921 atccgcctgc acctactgct ccagccatgg tagtcagtcc gatcacgttg atgatagtca
    31981 acgccgagac ccgaatgata ccaggcaaac cttctaacaa atacacacgt aaaatgatac
    32041 cgattggact ggttcccatc gcttctgccg cttcgatcac cccaggatca acttctagca
    32101 gtgcgttctc gatttgacga gcaaaaaacg gaacaactcc aaaaatcaat ggcactaaag
    32161 ctgctttcgc accaatcgtc gttccaacta agaaacgagt agtcaatgct aaaagagaaa
    32221 gcaagataat gaaagggatg gaacgaatga tattgatcag cttatcaagt atttcaaata
    32281 gcagacggtt agctagtaat ccattagggc gtgtcactac taacgtcaca cctaagatga
    32341 ttccaatcat cccgccaatg aatgctgtcc aaaaagacat atatagtgtt tgcttcgtcg
    32401 cttcgacaaa ttcatccgga atcaaataaa cattgggcaa atatgtttta aagaaatcag
    32461 tcattttatc ttcctccttc gatcactttg atacgactat ctaaatcgtt tttttgtaaa
    32521 atatttattt ttacaccaga tttgatcaaa taatccaatg cttttttccg ctgtttggtt
    32581 tctccagcta aagtaacgat caaactccct aacggcgttt cttgtaataa ctctacattg
    32641 ccatataaaa tattcgctgt tacttgatat ttgctgtaca gatgagcgat tagaggttcg
    32701 ttcgtctgat ttccaatata acttaattcc accagccatt tattggcgat ctgatcggca
    32761 aaagcagaat gggcaatgat cgtttctaat gcttggtcaa gatgagtagc tgttcgaata
    32821 aaatctttgg tcaattcttc ttcaggattg ctgaagatct ggatgctgct acctttttca
    32881 acgattcttc catcttccat aaccgctacc ttgttgcaga tttctttgac aacttgcatc
    32941 tcatgagtga tcaggacgat tgttaaacct aactggcgat tcaatttttt taacaatgca
    33001 aggatctgaa gggtcgtttt cggatcaagt gcactggttg cttcatcaca taataatacc
    33061 ttaggttcat tcgccaaagc tcgtgcgatt gctactcgtt gtttttgtcc ccccgacagt
    33121 tgtttaggat aagcagatgc tttttcttcc aaaccgacaa gttccagtag ttcattcact
    33181 ttttgacgac gttcctgttt cgattttcca gaatacttta aagaaaaatc aacgttgtca
    33241 aaaatagttc ggctttccat tagattgaaa tgctgaaaga tcataccgat ggcctttctt
    33301 tcttttcgca attctttggg agagagctga ctcagattcg tctgattgac aatgacttct
    33361 cctgctgttg gtttctgcag caaattcatc acacgaacta acgtactttt ccctgcacct
    33421 gagtaaccga cgattccata aacatcgcct ttttcaatcg ttaaatctac agaatctact
    33481 gcctgtatct ctttttcttt ttgtttgaaa catacagaaa catctcgcaa ttcaatcatt
    33541 aaaattcctc cttatctaaa aaaagcgtcc tcaagaaaat gaaaatgtca ttttcttgag
    33601 gacgcttttt tgcgtgttac cacctcatgt tcaagctgtg ttcacacaca actcctcttc
    33661 aagtacgatc atactctagc aagatatcgg tcgcagccgt catcgcctta catctatgct
    33721 cgggatgaat tgctcgatgg ccattttcca atagtcgttc cttgcttctt ctcagctacc
    33781 gaagctctct gtcaaaggtt cttctatcgt acttaccatt tcaatgcaaa taatattcaa
    33841 aaaaaaagtc cttttactaa cataaactat gtcagtaaaa ggacgacgta tcgcggtacc
    33901 accttttttc ggaagtttcc ttccctcaag cccaacatct caaaaagaga gattggcgtt
    33961 cgttttaacg gtcgaacagt tccgaatttg cctacttatc gacaaattat tctccaaggc
    34021 cattttcagc ttttcttact tgactcttct caccagccag agcctctctg caaagtttcc
    34081 aaacttactt tccttttcaa cgaaatcgat attttgttgt tgggattaat ctacatgagg
    34141 aaaactgaaa agtcaagtac gaaattcaaa ttattttttc tatatttatt tttctgtttt
    34201 tttatttaac atttcaataa cagttcgaag atcttttcct tccgcaagaa gtttcaattt
    34261 ttccttttct tcgacggtat aatttcggac aaaactggaa tcaggtatca aaacaacgcc
    34321 taatcctgct cgtgaagcag ctgttgctcc tactaaagaa tcttccactg caaaggctga
    34381 actagcttct aatcctgttc gttccaaagc tgtcagataa ggaatcggtg aaggcttgtt
    34441 ctccgacact tcgtctccga atgtaaacgt gtccaagtaa tcagctaatt taaagtgatc
    34501 caagatatct gttgctcttt tcgtaacagt ggtcgtagca atgcctagtt tcatatgacg
    34561 ttcttttaag tctgccaata tttcttttgc atatggtttt aagtggatgg ctccgcttgt
    34621 cagctgttga taaaaatatt cttcacgctt cgctcggact tcctgtactt tttcagggct
    34681 acctgctgct ttcactaaaa tatcaaaact ttgtttccag ttttttccgc cccaagcgtc
    34741 aacgatttct tttgggatat ggatatcaaa ttgctgaaat ccccaaagcc agccatcacg
    34801 atacgttttt tctgtgtcaa ctaacaaacc atctaaatca aatattgcac ctttcatcta
    34861 tacattaatc ctttccaaaa caatacacat agtttagcac gactggtcca aaaagaacag
    34921 aagcaaagaa ccagccttct cgtttataag agttcttgga atcgacggat atagattttc
    34981 ttcacagctg tcacaagcgc cagataagcg atgactgtag caaataacca taaccagtaa
    35041 ttgccaggaa gtggcatcaa gcctaattca gcaccaaaac ttgtaaaagg aaggacagaa
    35101 ccgacaataa ttcctaatgt agtgataaca aacatggcaa atgttgcgtt gctttggata
    35161 aatggtactt tcggtgtacg cagtgcatgg agcactaagg tttgtgtcca tagtgattcg
    35221 acaaaccatc ctgcatggaa taatgcgatg aaaatcactc gctgtgaact atccaatgta
    35281 tggaaacttc cgcccactac agcaggacaa ataacaaaat acatcaacaa gtaagtagta
    35341 atatcaaata tcgaacttgt tggaccaaag taaaccatga attccccaat actagatgca
    35401 ttccattttt ttggctcgtc caaatattct ttatccatcc gatcccatgg caaggaaatg
    35461 caggaaacat catagatcag attcaaaaac aaaagttgta aaggcagcat tggtaaaaat
    35521 ggcaggaacg tacttgcaat cagcacggaa aacatattgc caaaattaga acttgctgta
    35581 gccttgatat atttcatgat attcccaaaa gtttctcgtc cagaaagaag gcctctttcc
    35641 aaaatcgtca aatctttttc taaaaggatc acgtctgcag attctttcgc aatatccaca
    35701 gctgtatcta cagaaatacc gatatcagcc actttcattg caggagcgtc attgatacca
    35761 tctcctaaaa atccgacagt atgaccattt ttacgtaaag ctgtcgtcaa acgtgctttt
    35821 tgctgaggat tcaattttac gaatacttca tggtcttcag ctacttgtgc taattcatga
    35881 tcactcattt ccgagatttt ctcaccggta accaattcat tgatctctag acctacttga
    35941 cggcacactg aacgtgtaac taattcgttg tccccagtca gaacttttac agctacacca
    36001 tgttctttca gagctttcaa cgcttgtttc gttgtttctt ttggtggatc aaggaaagca
    36061 agataaccaa tcaaaaccat gtctgactca tctttgactg aaaattctcc aacgacgctt
    36121 ggattggtct tttgtgcgac accaatcact cgtaagccat cttcattaag tgactgtact
    36181 tttgcaagta cggcttcttt tcgttctttt gttaatggtg taacagtacc gccaagatca
    36241 ataaatttag aaatgttcag catttcttca atcgcgcctt ttgtgatcat ttgtgtttta
    36301 ccagctggat cagcaaccac aacactcata cgacgacgtt caaaatcaaa aggaatttca
    36361 tccacttttt gataattgat cttatctgta tctaaagttt ctgttgcttc atcaatgata
    36421 gccacatcta acaaattttt cagccctgtt tgatagtagc tatttagata cgcatggcgc
    36481 aaaacacgat catcttcatt tccttcacaa tccatatgat attcaagaat gattttatct
    36541 tgtgtcaatg tccccgtttt atccgtacat aacacatcga ttgcaccaaa gttctggatg
    36601 gcattcaaat ttttgatcac tgttcctttt ttggccatca cacttgctcc tttgaccaaa
    36661 ttcgtcgtaa cgatcattgg aagcatttca ggtgttagac caactgctac agatagtcca
    36721 aaaagaaaag cttctagcca atcccctttt gtaagcccat tgatgacgat aactgctgga
    36781 gccatcaatg acataaattt gatcagtagc atggatgttt tatggatgcc taattcaaag
    36841 cttgattgaa ttggtttttc agatagcgtt tctgctacat gaccaaatag tgtttcattt
    36901 ccaacagaaa caatgattcc ttccgcactt ccactgatga cattacttcc cataaagaca
    36961 agattttcgt aacttgtttc gctttgctga tcgtcttcac atactttcgc atgcttttct
    37021 actggatagc tttcccctgt caatgctgct tgggaaatga ataaatcctt tgcggacaat
    37081 aaacgcatat ccgctggtat catatcccct gctgaaagcc gaacaagatc tccacagacg
    37141 atttcttcca taggaatctc tgtgtacttt ccattacgtt ttacagctgc cgttactttc
    37201 acaagtgatt tcagtttttc agccgcttga ttggaacgta cggactgaat caacgtcatt
    37261 gtcccgctga cgataaccat agtgaagata atggctacac cgaaaagatc tttatctcca
    37321 ggagctgcta atacatattc cgtgatgaat gaaataattc ctaatgcgat caacactaat
    37381 gtaaatggcg tgatgtatgc tttcaatatt tctacaatga atggtgtttt tttgccatat
    37441 gaaatcgcat tttcaccgta attttctctt gaacgttcga tttgttcttc tgtcaatcca
    37501 tttgctgatg catggaatag ttcaaataat tcttgtttcg tttttccagc aaaagctgca
    37561 tagatttttt gtgtatcttt ctttttttcc atctcaatct ctcctttgtg tccaaaagac
    37621 agctgttctc gctgcttttg gtctttagct attcatcgga tcgatagaga aaaaactgaa
    37681 aggggctgag aacagataga taatagccct ggcctttcct ctagatcctt gtttttcgaa
    37741 tgactgtcat ccatttgttc tcatctcctt tactaagtaa ttttaattca tgacttccaa
    37801 tcatttttcg aatagttatt tttccaatta gaccacctcc tttttatttt tcaccgatgg
    37861 ggatttgcgg caacaaaaaa acctctcgcc aaatactcag cgaaaggttt gaaaaagcat
    37921 attaaaaaaa gcttctcaga tctcatcgtt gagttttgac actgtacaac gtaaaaagca
    37981 gatacctgct tttagctacc ttatgaaaag ccttatctcg acaattcctg ttctacccat
    38041 tggcgtctct cgacgttttt gggcagtagc ctatgtttgt ataggagcct cacctaacaa
    38101 atcttattcg gttttccttc gttaatataa caactaaaaa agaggatgtc aaatcaattt
    38161 tccatgtttt tcatactttt tttaaaatac acacgttata ttcctgtctc acgcggtttt
    38221 atttgtctca ttttccacct cccgctataa tagaaatata gtaggaaacg aggtgctgtt
    38281 atggaaagaa cacttgttat tatcaagcca gacggcgtga gacgccattt agtcggaagc
    38341 atcatccaac gttttgaagc aaagggactg gccattgctg aaatgaagtt tgaaactatg
    38401 actcctgaat tagcaaaaga acattatcag cacttgactg agcgcagttt ttttgatgaa
    38461 ctgatcgatt acatgacttc cggtcctgtc gtttatcttg tgctggtcgg cgaggaagtg
    38521 atcgatatcg tccgtaagat ggttggagca acaaaagctg ccgatgctgt tccaggtacg
    38581 atccgaggag actacgcgct gccggggact gaaaacatca ttcacgcttc tgattcccgt
    38641 gatgctgcgg taaaagagat cgcccgcttt ttcccgccat tcgatataaa caaaccgtct
    38701 gaaaaagcgg cgaagtgata caaattctaa actgctggtc tagttgctgg gttcagcaat
    38761 ggccagcagt tttttctcta aggacctccg ttgattcttt cctaacatat attcgagcac
    38821 tgtgaagaat tcttggtagt ctttcgtctt ttcttcccct aaagcagcag aaatcaatct
    38881 ttgataataa tctgccatca atgtttcata gatcactgta cttctttctt tatgtgataa
    38941 gctccgtttc atatcttcat tcacactacg tgcctttttc aaccgaccat tttctatata
    39001 aaacgaagtc aggttcatta aaaaagtaaa gagatactgc tcatgattgt tcaactgtgc
    39061 taatcgtcgc attctttttt tgattttcaa ataattgttc tcaatgaacc gatcactgaa
    39121 tatccaagga aaatcagcta gaatagatag ttcaaattga ccatagtctg gtatctgcat
    39181 caagtattct tcaatgattc ggcattcata acgtaaaaat tcaaattctg tctcagatac
    39241 ttctgttttt tcgaccatgt gaagattcaa aaccaaataa tacagatctt ctgttttctt
    39301 aaaatgccta cggttattgc tgatctcttt ataaggatca aagctgtctt cttgtacccg
    39361 atctagcatt cgtctttttt tctttaactt tattggttca tccagctggt ttcgcatcca
    39421 ttcatattct gcaagtgtca tgttcattcg ctctaaaaat gatctcaatg ttacaaatga
    39481 catttttttc actgaatttg tctcaatccc gactagagaa gtcttaaaac agattccttc
    39541 cgctaattgg gactgtgtca acttcttgtt gactcgaagt ttcttgatca attcaccatc
    39601 atacataata gttctccttt ttaatttaaa tttttccaca ttcaataaat agatacgtta
    39661 aaaaggaaaa atacacgaaa aaaataaaaa aatgtttttt tcataaaaaa taccaacctt
    39721 caagcattag aatgttgtct aactactgaa ggttggtttg attgtttatt gattattttt
    39781 ttgctgttca acgtctgttc ttgttactgg atataagttg gttgttgttt tattaccatt
    39841 ttttgaaaag acacttgtcg aatgttcgcc tttttgcttt tcgattgctt cattacgttc
    39901 cgcttcattt ttatagtcat attttttcgg atcaatcgtc tcaaatccgt ttggcacata
    39961 aaaccgcaag agattttcct gattcagctt atctgaaaga cttaacgcag tcttgacttt
    40021 ttcctgatct ttggagattt cctcatcgat tcctgctgct ttcgtatcga tgatttctcc
    40081 tgtctgatta ttgtaagctt ttcctcctag gacggtgtat ttgggtgtca caaagttccc
    40141 atttcgaaaa gcaactactt gatcatgctg agaagataat aagtctgtac caaaatgaag
    40201 ataatcttta tcatcgattc ccaatagatg tagtaatgtc ggcaaaacat cgatttctcc
    40261 accgtattca tgattgactg tccctttagt ataaccaggc atatggatca tcaatggcac
    40321 tcgttgcatc tgtgcgttgt caaattcatc ccatgtatct ggatctttac caagagcgct
    40381 tgctaaatct ttattatctg tattcgagat cccaaaatga tccccgtaaa tcacaaagat
    40441 tgaattttgg taaataccag attttttcaa atacgtaaaa aattgttcca atgattgatc
    40501 cagataatgt gccgttcgaa cataattatc tactgtgcta ttgccagtat tcaaagatgg
    40561 aaagtcgaaa ttcttgtcat ccgtataata aggcgtgtgg ttggttacgg aaagaaattt
    40621 cgtgtaaaac ggctgttgta gatgctcaag atattgggca gattctctga ataaatcttt
    40681 atctaatatc ccataaccta acgtttcgtc ggattcatca aaataagatc gatcaaagaa
    40741 attgtcataa cctaagtttt tatatacatg atctctattc caaaaactag ctacattccc
    40801 atggaaaaca gcgcttgtat agccttgctt ttgtccaagg atcgctggtg cggcttgaaa
    40861 gacattgtct gacccaagtt ctgtgaataa tgagccttga ggtaaaccaa atgtgccagt
    40921 ttccaacata ttttcagcat cacttgtctt tccctgtcct acttcatgaa agaaattgtc
    40981 aaagctgatc gttgcttgat tctggaaaat actatttaaa aacggtgtga cttcttgtcc
    41041 gtctaccttc atattaatca aaaactgctg gaaactttct aaatggagaa cgatcacatt
    41101 tttcccttta gcgatcccaa atgtttcggg atttggctct gcataatgtt tctttgtata
    41161 atctaaaact tcatcaatcc cattactgct tgcatgcgcg cgaacactgc tagtcattcc
    41221 tgttttgatt ccgtcataaa tagtatacgc atcgattcct agatatttga caatatatga
    41281 acggtcaaat gttcgctgca gcaattgtgg acggttggct tcgctcagcg ctagatttcc
    41341 tgaaaaaata agccctgaaa gacaggtaac agcgatagcc atcggtttgc cgacagggtt
    41401 gcttttgata ggtattttct tgaccttcaa ccaaaccagt agcccaatga acacagcgat
    41461 atccagccag tagatgacat catggggctt catcaaagaa aaggagcttc ctgacaatcc
    41521 ttgcgacact ttcgaaaagc ctaatacaga tttgactgta atgaaatctg tgaattcccg
    41581 ataaaaaata atattcaagt acaacaaaag cgtatttaaa atatcaatga tcaaaagaat
    41641 agctaacgct ggctttggct ttttaatata caaagaaagg ccaaaaaaca ataaggtagt
    41701 cgcaattggg ttgatccaca agataaagta ctggatcgta ccttccacac ctaatgaaaa
    41761 atctgcatag taggcaatga tcgtcttcag ccagaaaaag aaaacagctg tcaaaacgat
    41821 tgctaaacga ttttggcaca attttttaag ttgtgtcacg tatatcactt ctctctattc
    41881 acattttttc ttattttgct cctgaaaaat gaaaatttcc taaaaaagtt gttatcttaa
    41941 gattttttta ttctttctaa agattattcc gtccactttt cacagcataa aaccgctgcg
    42001 atgtaagcga ttaaactaat gaagagataa aagtcttgtg gtgaccgctt cactccacgg
    42061 tattcaaaag ccagtttctc ggtattcgta caaaaaaaga ggctgtgaca aaattcttgt
    42121 cgcagctcct tatttcttga tgcaggacag ctttttcaca acttcttcct taaacggtgt
    42181 tctcctgcca actcctcggc cacaagtcac aatatttata aaacatgaga agctgttttt
    42241 tgaaattgtt ccttgtggct cagagttaaa cggcaggctc aagacctctt gatacacggt
    42301 gttcaatcag ctgaccctcg gtattagatt atgtcaggac gctaatttta caacctcttg
    42361 gtaaacggtg ttcaaaagct tgcatctgcg gtaataagcc caaccaaacg aaaaatataa
    42421 ggagctattt tttgaaattg ctccttgtgg ctcagagtta aacggcaggc tcaagacctc
    42481 ttgatacacg gtgttcaatc agctgacctt cggtattagc tcaaaaaacg gcaaaatatg
    42541 aggaactatt ttgccgtttg tttctctaat actcaggtca aaacgctgat ttcacaacct
    42601 ctgtttttca ttgtccatag gattgatatt gtccttgtga tgactcattc gtaataattg
    42661 aggcttgatt ttcttcactt cccgttatcg tttgtccact gccagaatct gaaggacttg
    42721 ttcctgtcga ctcgtttttt tgagaagaga cagttgtttt cttgacttgt tcatttgtct
    42781 ttttccattc cgctaaacga tcggcgacca cttttttgtc tactttttca gaataagcgg
    42841 tgatcacttt tccatttttc aaacgggcta ctttgtcgcc atcatgagtc aaaagcaaaa
    42901 cggtcaaatg cttgtcttta aattgatatt gtctgagaga aatcgcacta agtacgtcat
    42961 cgatattaat aaattgattt tcttcttttc cttttggcaa ataaatgtcc aattcgcctt
    43021 tttcgtaatt ggcagttatt ttatactccg cccaccaata agttgagaat tcttttttga
    43081 aattatctac tgattctttg cttgctacga ttttttctgt ttctttctga gcaagatctg
    43141 ctgtcatttt tcctgaaaat gctgcggata aaataagcag taagaaaaaa ccgaataacc
    43201 aaaagagaag aaacggtctt tttttcctca tgctttcctc cactcctatt taattttgac
    43261 tattctatta tatacaaaaa atgaaataga aagacaaaaa agttgaaaaa gatcgtaaat
    43321 aattgaactt ctttttcaca aaatggtaag aatggagaat ttcacgcatt tgctctcaac
    43381 tattctttct taagcctcaa atccgttttt atctgatacg tctttgaacc aatgacccga
    43441 ttttttgatt gttctttttc catcttgtgc taagtctact gaaataaagc catatcgatt
    43501 cttatatgca ttggtccacg accagttatc catacatgtc cacatatgat agcctaaaca
    43561 attggcacct tcttgaatgg cttgatggac atatttcaga tgagattgga caaattcgat
    43621 ccgataatca tcgtggatca tgccatcttc tttgataaat cgtgcttcgt tttctacacc
    43681 cataccgttt tcagaaataa agcagtcgat atttccataa tgatctcgta cattgatcaa
    43741 aatatcatag attccttgtt catagatctc ccatccgcga taaggattca ttttacgacc
    43801 aggcatttca taattgtcga aataatcctc aggcattgga ccgtttttag tatcgattgg
    43861 tgtttctttt gcttttaccc ggcgtggctg gtaataattg atacctaaaa gatctactgt
    43921 attttctttg ataaccgtca aatcctctgc atggatcaca ggcatgagat caagttcttt
    43981 tactatctca atcaattcac ttggaaactc gccttttaca gctggatcaa ggaatgaacg
    44041 attgaagaac gcatctgcta tttgcgctgc tttaacatct tcagggtttg attcgtctct
    44101 tggatagctt ggtgtcagat tcaagatgat accaataaca ccaccttgct ccatttcatg
    44161 gtaagatttg atggctaacg ctgaagccaa cgcttcatga tagccaactt ggatcgcttt
    44221 tttcatatct gactcttctg gataatgcca tcgatataaa taacctgctt cgactggaac
    44281 gattggttca ttatgagtaa accatttttt tactttcgaa ccaaacaatt caaaacaact
    44341 tttggcatat ttttcatagg cttcaaccgt ttctcggttc aaccatccgc ctttttcttg
    44401 cagatacatc ggcatatcga aatgatacag attcacaaag ggttcgatac cgttttcaat
    44461 caactcatca aaataacgat gatagaaacg aacggcttct tcgttgattg ttccagattc
    44521 atcaggtatc aaacggctcc attggataga tgttcgaaat gaattatgcc cagtctgttt
    44581 catcaactgt acatcctctt gatacttcgt ataaacttgc gaggtttttt caggtcctac
    44641 ttgatcaaag aacttttgcg gttctttttt gtaccaataa tcaaaaatgt tctctccttt
    44701 tccatcgcct gaaatccttc cttctgtttg tgggccactt gctgcagaac cccaccaaaa
    44761 atttgctgga aattgatatt ttttcatcaa agcacctctt tttctttttt agttcgtttt
    44821 agttgattcc atgtatgata ttttccatca caatcacttt ttcattttct tcctcttctt
    44881 ttttgattcg ttttaacagc tgttctgctg ctcgttcccc gattttatct gttggtggcg
    44941 tgatcgtgat tttcggaagg atactcagat cgtttttatc taaataatca tagtgactga
    45001 acgcaatatc cttcccgatt ctgacacctt cttgattgaa acattcgatg gcaccaatcg
    45061 acatattgta attgctgaca aaaatagcag ttgccccttt ttctaataat gcttttgccc
    45121 cgtaatagcc gctttttcta gaatagtctc ctgtaaagac ctctaattgg atcgatggat
    45181 cattttggac cgcttctttg gctcctgcta aacgttctct tgctacgtag tctgtttgag
    45241 tggcagcaat aaatccgatt tttttatgtc cttgttcacc atgatgagcg atcacttttg
    45301 ctacactttc tcgatcattc actaatattg tatcaatacc tgctggtcct tctggacaat
    45361 tcaatgcgat aactggaata ctcatttttg tgagcatttc aaatccagac cactcttctt
    45421 cggataagaa gacaatcagc ccatcgatca catgcttcat caggtattca attttttgtt
    45481 caattttttc tggctgatcg gcaaacccgc ttaataataa gctataccca ttttcttcag
    45541 ctactctttc gatccctgag acaacttccg acccaaaacg gctggaaatc ccgtttatca
    45601 aaagtccaat agagaagctt cgattattct tcaacccttt agcaatgatg ttttctttat
    45661 agtccaacgc tgaaatcgct tctgctattt ttttcatgtt cttttccttc agttgctgac
    45721 cattcaagta acgggaaact gttcccactg aaacaccagc catcttagcc acatcacgga
    45781 tcgatgccat acattctcac tcctgccagt tacatttgat ttctctttct tgttgttcga
    45841 ttatgatgac cgcattcttt gcatagtgtt gcttgtcatc aatatccata taactaaagt
    45901 cttcagtaga gcaacttcct ctcactatta attatactta aacaagtatt tgaaccggtt
    45961 catttttaat atagcatttt tttaaaagat tgcaacactt ttttaaaacg ctttctgaaa
    46021 gtggcggatt ttttgccttt ttaaaatttt attctaaaat ttactctatt tgtgggtgtt
    46081 ttgtgcgttt ttctcttcca tccgtttgaa gttttacatt ccaatggtat aatttcttta
    46141 tcctatcaaa tgggggaagt attttgaaga aattaatcgt actatttatt ttgtctgcgg
    46201 gacttttgac attgactggc tgtgatttgt ttggcggctc tgaccaatcc tcggacgaaa
    46261 caaccgattc ttctgtctta aaagcggaaa aagcagatac caccaacagt gtatatacgc
    46321 taaaagtacg taaagatatg gatttatccg cgggacagtt gacatttaca gataaaaaaa
    46381 tggaatggac acgcacgtat cagacacaag gaactgattc ttctgaatca tctgaagaaa
    46441 aaacagtgct gacagatatc aagatcacta ccaaagatga cgaatatatt atttcaggaa
    46501 aagaaaataa aaaagaaaca acagtcatat ttacaaaaat cggtaattac cgtttgaagg
    46561 atgaggatgg caacatctac agtctatagt gccgtctgtt ggatcttctt ttgtttctcc
    46621 ccttcttttc tgctatagta aagaagcaag atgattgtaa aggagacgaa atggatgatc
    46681 gatagtcctg attattgtac atgtaataac aacaaaaata tccatacaga atttaatgac
    46741 caaggaagct tgagcatatg cgacaactgt cacaaactag tcaaagattc atttaaaaca
    46801 tttgaatctt atcctgttca aaattatgta aaaagtagaa gaagataaac aaatccaagc
    46861 aaaactcact atggctgatt tttatgaact tcaaactcag cttcttcctt tgatcaagct
    46921 tcatcagtaa aacaaacagt ggatcatttc agtttacaaa tcaaagagta aagatttatt
    46981 tatttcaaat atttgaatat aaaataacca aacgatagaa aggcttattc tacatcacgt
    47041 agtaattttt ctttcccgtc gtttggtttt ttatttttag cctaattctt gaccttttgc
    47101 atgctggtta tccgtaaaat attttccagc aggctttcct tccaattcag ctaatttttc
    47161 ttggatctga cggatcacct tttttgcatc ttctggattt tctacaaaat cgaatttatc
    47221 cccatccact tgtattttag gacatacatc aaaatcttca taccattgat tatagcgggt
    47281 gttcaacgtt tcataatagg tatataacgt ctcatcgctt tctagctgtt catagtcacg
    47341 cccacgtttt ttgatacgtt ctagcatcgt gtccaaagaa actttgatat ggaccatcaa
    47401 atcaggccgt ttttttggag caacatcatc taactcgttt agcatcgtat ctaataagct
    47461 atcgtactgc tgtacttcga tatccgttac ccgacctaaa tcagcgttta gatggaacaa
    47521 caggctatct tcatagatgg atcggtctag cacattgtca tcgttcacta aagcattttt
    47581 cattgctaaa aatcgcttgt tcaaaaagaa gatttgcaat aaaaaagcgt actgctcagg
    47641 atttgaataa aagagcggca atacttcgtt atcttcgaca ttttcataaa atggtttaga
    47701 atcaatctct tttgcaagca tatccgtcaa actgctctta cctgcaccaa tcgttcctga
    47761 aatcaaaatc actgacatac aacccttcct cttctataag tactcgccta cgtaagtttc
    47821 aacaggcaat gtttcaaaat catattcacg tacagcttgg tctgatttta aatacgtatg
    47881 gagactttgt gtaatcatca agttcgtagg taccgtctct tcttgtacaa tgatcatcgg
    47941 ttttttcaac atagttgcca cacctaattc gtaagccgtt cctggatcga ttgtttgatg
    48001 ctcaaagtca aggatagcaa caacaaattc tgcatttgtc agttcttcca tatctttttc
    48061 gtatacttcc tgcgcccatc cagcgctgaa tagttcataa ttgctttctt ggtgatgacg
    48121 tggagagtag aaacttgtga ctgttttgtt ttcctccaac gctttttcga tacgacttac
    48181 tcgatcgatt tgctcctctg aaaaaaatgg tcctgctaaa tatatttgac tcattatttt
    48241 gcctccttca ataataactg atttttaata gatactgctc gtaattcatt gaccagttcc
    48301 aatggattgt tggaaaagat tacttgatcc aaccattttt tatccataaa atcctctttt
    48361 gcaaaagttt ctaataattg ttgcaagccg ctgtaataac cgccaatatt caatattccg
    48421 attggcttgt cgggcgtcag accgattgct atccaagaaa gaagttgtga gaattcctcc
    48481 aatgtaccta gacctccagg gaaaacgagg acagcatctc ccttttcaaa taataatacc
    48541 tttctttcat cgatcgtttc tgtaggaata aatgtagtaa catcttttcc aggcaattca
    48601 gtttcaaaaa gcccattcgg atagatacct gtgacttttc cgttattttc cagtactgtt
    48661 ttactaatca ctcccataat cccggagtat gaaccaccgt aaaccagttc gatttcttct
    48721 tgtgccatgt attttcctaa cgtttgcgca ataaatttgt aactttcttt attaccaaaa
    48781 cgggaaccgc aaaaaacagt aagtttcata aaaaaacact ccctaactca aaagtctatc
    48841 ttcattctac acgtgttttt ttaaaataaa agactagatt ttgtagtttt ttagtggtat
    48901 ataggataga cgccactata tatagtattt agaaaaaaat catttttcag ccattttata
    48961 gataaaacaa aaataaataa cgtgttttca tcgatatgaa atgacttact tatagaagat
    49021 actgggattt cgtcctatat gccaataacg tgtattattt taaacaacaa atacttgttt
    49081 cttttattaa cgaaaaaatc attttgttct cgtgttatgt ttcactgata aatgatgttt
    49141 ctctaaataa aaaagccgac gaactaaacg atcaaaattt gatcattttt gttcatcagc
    49201 ttctcctaaa aatctttttg ttaacgataa atagctggat ttgtaaaaat atcacttgaa
    49261 tcgtcactgc tggaagaaaa ttcagtgata accgcacctg ttgcgcctgc ctggaactag
    49321 tgtttgatat tgggctcgaa cgtgtactga tctctagcat gaagaataat ttcttttttt
    49381 actgtataat aactttcttt cctttttgga atagtacagg aaagtttttt tgtttccttt
    49441 ccttcgacat aaggtatacc agaccctttc gacaacgaaa ggttctcatt tttccttcat
    49501 cgtcatttca aggagggtgc cgatgctctg gacagtttcg atcgctccta aaccaaaatc
    49561 tgtatattcg attctttcta actctgattc tttcatacca ttcctgtctc aaggtacatc
    49621 tattgtactt ttttcttgta atccattctg cctctcctaa ctaattgtca aatcatccca
    49681 agatatgtct tcttcctctt tttcttcttg gatgatttct gcattcggat ctactggttt
    49741 tttcaataat aaaatggtta ttcctgtgac aaaagaacca gctaacaatg caccaatgaa
    49801 tgcccatggt ctggtcatcg tagcgactgc aaacattccc gatgccggaa tacggctatc
    49861 tgcgccccaa aacatgctta atccgccacc tactgtccca ccgactcccg ttgctatgac
    49921 attgcgtgcc aagtcgttca atacgatagg gaaacatccc tctgtgatct gacagatccc
    49981 cataggaaaa gcggtcttca atgtatcgat ttcttgacga ttgagtatat ttttacgaat
    50041 gattttccct aagaaataag cgatcgtcaa gccgaatggg gttaccatcg aagctaaaat
    50101 caatacagtg attggttcat agattccttc tgaaaatcgt gccacacccc cttatccaga
    50161 ataaatggta atccagaaac agccgtgtat ctgcggtaat aagcctaacc aaacgaaaaa
    50221 tatgagaagc tatttttgtt tggctgttct tattacttga tgcagggcag ctttttcaca
    50281 acctcttttt ttatttatgt ttcataatca atatggtaag gatgcagctc cgtatggtag
    50341 accgcctccg aatattcaat catttcccca atttgcgtag acgatttccg tatccgtttg
    50401 atgccgatct gtgcgtctgt tttcagaatt ccttgttctt cttctgttaa aaatactgcc
    50461 gagaaatcat cagaaaatga gtcaatagta tatccttttt tcgtcagcaa acgatacaaa
    50521 gattctttgc caaattcttc tctgctgaat tgcatcattt cttgtggaag ataatgaaca
    50581 aaataaatat aaggctgatt gttcaaataa tataagcgct taaaacaaac agcttgctca
    50641 ccgaaaaagg aaaacaaagg atcttccttc tttatatgga ccaattgcaa atccagcgtt
    50701 tctttccgga cttcatatcg ggagttctcc aagatttgcg taaagcttgc cgctttggat
    50761 aatcgattgt aaggacgatt gcttagaacc gtcgtcccac gaccactttt ctttgcgaca
    50821 tattcatctg atgccagcag ttcgattgcc tttcggacag tgattttgct gacttgaaac
    50881 agttcttcta attcactttc tgtcggcaac atcgaaccga ctggatattt tcctgagaaa
    50941 atatcttctt taattttatc tgcgacttcc aagtatagta cacgtgtcct tttcataacg
    51001 tcaccctttg ctttactatt actaaaagaa tagcacataa aaaataaaaa aagaacacaa
    51061 atcacatgtg agaagtgtgt tctttttcat tcagctgctt taattacaag tttacgccat
    51121 ttgaggcaat tacttcttta taccaatcaa aagatttttt ctttgatcga ttcagtgtcc
    51181 catttccttc atcgtctaga tccacataga taaagccgta acgtttactc atttctcctg
    51241 ttgatgcact gacaagatcg atacatcccc aaggcgtata gcccatcaaa tctacaccgt
    51301 ctgctactgc attagccatc gcttcgatat gacgacgtaa ataatcgatc cgataatcat
    51361 caataatcgt gccatcctct tccatatgat caatggctcc aagaccgttt tcaacaatga
    51421 ataatggttt ctggtaacga ttgtataatt cattcaatgc gattctcagc ccttctggat
    51481 cgatctgcca gccccattca cttgcctcaa gaaacggatt ttttactcct cccagcaagt
    51541 tcccttctga agttgctgct tgtggatcag tctcattgat tgccgtagac atataataac
    51601 taaatccgat gtagtccact ggatattctt ttagcaactg gaagtctttt tccgtttgct
    51661 ctaatttttc cggatcgact ccatattttt tatgggtacg cgctgtgtat gcagggtatt
    51721 ctccacgtac ttggacatcc gtgcagaaaa aattgaattc ttggttttgg atcattgttg
    51781 ctgcctgatt gactggattg gcatcgatgc tgtaagttgt tgcatagata atcatgcagc
    51841 cgatttggat atccgaacgc aattcgtggc cgatttttac ggctttgcta cttgcaacga
    51901 attgattatg ccatgcttgg aaaatatttt ggtactctcc tgctccattg ctttttacta
    51961 atccttgact taaagctgga aagtgaaagg ccgaattgat ttcattgaaa gtcatccaat
    52021 atttgacttt actgctgtat ctctctaata ctgtctgagc aaatcgttca tagaagtcga
    52081 tcagttttcg atttttccag ccaccgtatt cttttgccaa atgaagaggc atttcgtaat
    52141 gagaaatcgt gatcacaggt tcaatatcat attttaagca ctcatcaatc agctgatcat
    52201 aaaattctaa tcccgcttcg ttaggagtac cttcgtctcc atttggaaaa attcgtgtcc
    52261 aggcaatcga aaaacgataa catttgaatc ccatttccgc aaacaaggca agatcttctt
    52321 tgaaacgatc gtaatgatcg attcctcgat gattcgggta aatatatttt tcttgatcaa
    52381 tcgtccagtc aaattcttca ctgccgataa tcgcaaagcg ctgcttacct ccaggcatag
    52441 catctgcaac ggatagtcct tttccgtctc gatcataagc cccttctaat tgatttggca
    52501 gcagttgctc caccccataa aaatttttct gaaaaacgtg attgcatcct gatttcctcc
    52561 ttattttaca attgccagaa catcttcgtt tgcaataatc tctttttggt ctaattcaag
    52621 tatatcggca aatgcatcag tattcgtaat gacaactgga gtgattacgc tatagccagc
    52681 tgctctaatt gcttcgatgt caaaacgaat caataaatct cctgctttga cgagatcccc
    52741 ttgtttgact aagatctcaa agccattccc atttaattct actgtatcca tcccgatatg
    52801 aatcaaaatc tccgctccgt cagtcgttgt gatccctaca gcatgtccag ttggaaaaat
    52861 agtcgtgatc tctccgtctg caggtgcaac tagttctcct acttctggat caatagcgat
    52921 accttttccc atcacgccag aagaaaaaac ttggtctggt acttcactca atggcagtac
    52981 tttacccgtt aaaggacttt ttaaggtatg ccgtgctgaa gtaatcggtt ctcctgcatt
    53041 cgcatgtttg ttttctaatt gattgtcttg tgtttgttcg tcaaatccta aaatcaatgt
    53101 acctacgaat gcaagaacaa aagcaattcc tgtagctatt actgctactg tcacatttga
    53161 ctctacacca tccaccgtat taatgaaagt cggaatcgtc aataagctga ccaaactgct
    53221 ggagaaagct tttactccgg aaaatccgat aatagctcca ccaatacctc cagaaataca
    53281 agcggcaatg aatggtttct tcaaaggtaa tgtcacacca tacacagttg gttcagtgat
    53341 tccaaataac gaagtcactg tagaagaaat ccctaatgct cgaagtttcg tatcttttgt
    53401 tcgcaatgct acagctaaag cagccccacc ttgtgccaag atggctggaa gaagcattgg
    53461 catcaataca tcaaacccat attgttcgat attcaaaaac atgatcggta cgaatcccca
    53521 atgcatgccg aacatcacga atacttgcca taaacttccc atgattagac ctgctacgat
    53581 tggactaaat ccataaatcg agttgaagag accgcctaaa gcattaccgg caaccgtccc
    53641 gattggtcct aaagcaagaa atgttaatgg aaccatcact actaaaacga caagcggaac
    53701 taaaatcatt tgcagaaatt gcggaatcac tttttttacg aatggctcta atttactttg
    53761 aacccatacg gctagaatga ttggtatgac agaagaagta taaccagatg gtgaaagaat
    53821 cactgggatc ccaaagaaac tgatagcttc tcccgcacca gcaactgctg tgatagctgg
    53881 atacacaagt gccatagcaa ttgctactgc taaaaactgg ttggctttga actttttagc
    53941 tgctgtaaaa gcaagcatga caggtaagaa ggtaaagaca ccatctgctg cagcaaacaa
    54001 tatttgataa gtaccgctat cagctgtcag ccagcccatc accgttgcca atgataagaa
    54061 tcctttcaat accccggcgg ctgccatcgc ccctagaaaa ggtgtaaata tactagagat
    54121 aatatcgatc agacgattca aaatattttt ggactcttct tttccatcct ccgaaactgc
    54181 aagaccttcc tcatccacga tcgattgata aacatctgct acattactgc caatcactac
    54241 ttgatactga ccgccacttt gtacgacttg gatcacgtcc gggtcttctt ttaacttttg
    54301 cgtatctgct aaagattcat cttttaatct gaatcgcaac cgcgtagcac aatgtactaa
    54361 acttttgaca ttcttttgtc ctcctaccgc ttcccataca cgacgaccaa ctgcttgatt
    54421 atccattgtt ttttccttct ttctttttta tttaaaaaat aaaaacccag attgaaaaaa
    54481 acagtcacaa acattcgtga acctattttt tcaatccagg ttttgcctgc catacagtaa
    54541 caatccttat gattggatgc tttttttctc acttactact ctttgaatat gaatcgttaa
    54601 ataaatctgc tctgcgatcc ccatttcctg tttttgcgtt tgtcgaagat actcattgat
    54661 ccgttcgaca caatgaaacg cttttggata ctttccttga actaatgcgt agagaaattc
    54721 atcttgctcg tcagatcctt cccctttgag ataacgctgg gcaaaaaact gcagatgagt
    54781 aatgatacgc tgataattca aagattcttc atcgaacacg atgccgaaga atttactgat
    54841 gatactcagt atatctctta ccattgtggt agcggtcatc gtcacttgca tgtcttcgtt
    54901 tccttgttgg ctattcacaa aatgaaaggc aataaaaccg gcttcatcct cagaaagttc
    54961 cacacccgtc ttttgtttca cgaacaaaag agcctctttc gctgcttgat attctttcgg
    55021 ataaaatttt tttgtttcaa ataataatgg attaggcagt tggatatttt cttttgctcg
    55081 tgtgatcgca taatgaagat gatcggtcaa tgtcagataa atattaggcg aaaattcaac
    55141 ttttagttta tcttcagcca gttcgactac ttgtttgacc cattcgatct cctctgccgg
    55201 aatatcagcc atcagctgct ggaattggtt tttagcaaaa gaatctctta aacaaattct
    55261 ttttggaccg cttctggcga aatctcgtcc ccaatctttt tttgaaaagc tagtcctttt
    55321 cccatacaaa cgacttcttc cccgaatcca tttttcgaaa tgacgacatt gttattcaaa
    55381 attttatcaa ttttcatttt gcttcgccct ctcaagaaaa ataaaaaaac ctagaattcg
    55441 cctactgttt cttagctgaa ttctggtttt gcctgccgta cagtaacaat ccatttctta
    55501 ttcaaaatca tcataccatc gaatttttat ttatgcaagc cttttcatat aaaaaataaa
    55561 aaaactggaa cacaagtcta tctagaaaac aaccaaatca gagatgcacc tctttgcgtt
    55621 tctctttttt tcagcaaatt ggtttttcct tatcgattca tatcctttgg ctgtttttaa
    55681 tctgtaacga cttatgtccc agtatcctaa aatgtttttg tctctagatt gaaagcagat
    55741 ctcatatcat ctgtcgcatc ttttatcgat gtaacacttt ccagttctcc ttgtacaact
    55801 agtcgcgtga cggcgtccat gtcgccacaa gtaattagtg tgacaatttc ttttccctcg
    55861 acttcattca gtaattgggt atctgtagga gcaacttttt ctttaaaaaa tgttttgtat
    55921 gtatacacat tttctaaatc tgtcaaatag atcttggcac ccatctctaa attttctagc
    55981 ggtgtaaaca gcaattcagg gttcgttgca cgatggcttg ctaaggcata atttcctttt
    56041 cccatttctt gatctggaga taatgttcct gctccgtaaa gtagtgacgt attatccaag
    56101 cctttgaaaa ccggtaaatt gattgaaact gaagggactg cgatactagc aatcactggt
    56161 agatctgttc ctctcatttg cgacctcatg accccttctg tcgtcattgg ttctaccgca
    56221 tcaaagtcaa aactgacatc tttatcgtta ttctttttca gatcttcttt tgttacattc
    56281 gctatagcat atttatctcc tgtctctctg acaaaataat ccttgatctg ttcattgaaa
    56341 atcaaagcta gtcctactaa taacaagagg aacaagagga tattgactag ccatcttttt
    56401 gcttttcctt tttttttcat tttctctccc tccagactca ttttactgtt tttcttttgc
    56461 tctttccaac agccgaatga cacatttcta caactctgtg tatttttttc gtacacctgc
    56521 gcttttttct acaggatatt tcaaattatt tttgatcaat ttttcttcaa tgatctcatc
    56581 tagatccagg tgaaggttgt ctgccatcat gtatgaatag atcaatacat ctgccagttc
    56641 ttctttgatt cgttctaatt gtgtttcttt tacttcttcc ggcgttttcc attgaaaaag
    56701 ttctaacaat tcagatgctt ccaaagaaat agagatagcc aaatcttttt cgttatgaaa
    56761 ttgtcgccaa ttacgttcat ctctaaactg gttgactttt tccatactat tcatttgctt
    56821 gccttcttcc aatcacttct tacttgatta atcataccat ttttttacat aaaaaataga
    56881 tgatgctttt aaaaaaactc ctgttttctt ttatttaaaa taaatgtcat agaaaatcaa
    56941 taatttaggt agccaccatg tgtatgcagt agcgtgtctt cttcccttga ggctttttgt
    57001 ttttcgatac gttcttcttc ccaaacagac aacgctacta gaatctctct taatgatttt
    57061 ccttcttcgg tgagcgagta ctctacttta ggcgggactt ggttataggc ttttcgatgg
    57121 atactttcat cttgttctaa ctctcttaac tgctgggtca gcattttttt tgtgatcgta
    57181 gggatttgct tgcttctcca cgcctaaaat aagcgatatt gctcttccct ttatctggtg
    57241 taagcaaagc agttagcatt ttgacgatcg tgggttccgc ttttcacttc ttatctactt
    57301 agtatcatga agtatacttt cgtactaata ctaagtagcg gaagtgtcaa ctactttgtt
    57361 taactacata gcaaaagaaa cagaagggtg acaacggtca tcacgcttct gtttcttttg
    57421 aatatgctgt gttgttgtct ctatctccgt gtagactttc ctcaaactta tcctttgaca
    57481 gtaagtcagt aggctgattt gctctttcta gatgccagat acatttctac aaaaaaatag
    57541 tgattctgtt ttgaaaattc gatttttagt tctgcatttt cgtaaacatc caacattttt
    57601 ttcgcactgt atagacctaa cccgccattt tttttagagg tttttcctct tttgaccatc
    57661 tccgatagcg tcaaattcgt tggatttgcc ttgttcgcta tactaaaacg tgatccctct
    57721 ttttcttcca gcagattgat cgtaatcgga ctctgatcgg ctacacgatg atcgaatgcg
    57781 ttagaaataa aaatagataa acagcgaata aaaacaacca gatcaatcgg gattgttttc
    57841 acttcgacag gaataactac ttggatcggg attttctccg attctgcttt ttctagaaat
    57901 tttaaaaaga cacccctgac agctggggat acaatatttg ttagctgttg tatttgcggt
    57961 ttttcaaaaa tagctgcaga atgatcttct aatatttcta aaaattccaa cgtttctttt
    58021 ttattttcct gaagagtaat ttttaatgaa aacagtagac ttttataatc atgtttgaat
    58081 tcctctagat gatggtaata ttcttcattt atttgataca gttgaagcga ttcctgatgt
    58141 tgtctttctt ttacttggaa actgatcaac ataatggaaa aaaacaaaga aaaaaaactg
    58201 atgactatcg acaaaagaga tagaagcaga taattagatg agcggaagtg aacacttccc
    58261 aataccaagt aaatcacgcc ccagcttaat aaaatacaaa tacctccaaa atagtgttca
    58321 gttggataag taaaaagcaa gttgagaaat ctcgtctgtt tgtctatact ttttactaat
    58381 agatagaaaa taaatgcgat aagtagtgca aaaaaacttg ctgttccaag atgtctcttt
    58441 cctaaataat ccatcagatc aaaaaccaga aaaaagctga gtccagtgat agcaaaaaaa
    58501 agattactga tcaaacagct gataaacagg tttttcgtag taaaataaaa aagaaacatc
    58561 aataataagc caactaggta aggaaagctc actttaggga taggaagtaa aagtgtcacc
    58621 accatcaaag ctatacaacc aaagctgttt tttactagca gttgtttcga aaagaataaa
    58681 tagataccta aatctaaaat caaagtaaag gtcaaataaa gtgtcactgt tgcgaccatc
    58741 ttattcctcc attgttttgt ttcaaaatgt acacatattt ttttaatagg agcctgcata
    58801 tgaaaaagta ttttatcata gaagataacc agtttcacag cgaatggatc atcagagaat
    58861 tggtcaatca tcatatcgag aatatcgtgc agctcacttc tatctctcaa atcaatcagt
    58921 tgatccagtt gggcactatt gataatcacg atgttttctt tatcgatatc gacttgaagc
    58981 aatcactcac aggtattgat gtcgccgaac aaatacgtaa aaaaaatgca catagtaata
    59041 ttgttttttt caccgcttat acgaattttg cagtagagat catcaatcgg aaaatccttc
    59101 cgattggata tatcagaaaa gacaggaatt tatctgaaca aattcgtcaa gtgctagaac
    59161 agatcaattg gaaagaacag cagatcgtaa atgtcgataa aatctttatt gatcaaaaag
    59221 gtggaagtct cgtcctgtat cctcgggaaa ttttatatat tgaaagtatc aaaggtttga
    59281 aaaaccaagt tatggtcaaa acaactacag aagataaatt agtgaatact tctttaaaac
    59341 aattgaagac agagttagaa aagtatgctt actttttact tttaaaatct tactgtatca
    59401 acttatcgca attgcagaaa attgattctg ctcattatgt gttagaattt ttcaacggtg
    59461 actccttact cgttggtcga aagatctttg aaaagacaaa ggaacgtttc catgacttcc
    59521 aaaaaactgc ttcgagttga tgaataagag ctgagacgta agcatagcag atttcaggga
    59581 gtgggccttg aatcggctcc actccctgaa atcgttgata tgattttacg cttcctcttc
    59641 tgtaggagca tctgttaacg accatgtgaa tgttgaaacg tagttgtcta cagcataatt
    59701 ggatacagga acaaataatt ccactgattg tctcccttgt tccacattct ctcctaatga
    59761 ataaatccat gtgccaatcc cttcatttgc tcctgcagtt actagatcta ctgcctgatc
    59821 tgctgggatt tcgattcctt cagcgccagt tccagaaacg gtagatggca ttccactgct
    59881 attagttgcg ctatttcgaa tcacggcgtt tttgaatttc acctttgaac cagttagttt
    59941 attcgtatca gatgttgtac caataaattc tgttcgtttg actgataatt tccaaccggc
    60001 ataatttccg cttttatccg ttacttgtac gaaattctgt acttgtgttt gtgctccagt
    60061 actgtccgtt ccgctttgta atgcagcata tactgttttt gctgtaccta acttgattgt
    60121 tccaaaatca agatctggag cttggtcaat cgtcagtaat ccggatgttc cctgatttgg
    60181 ttcgtcaggt gtaaaaggag tatcaggatc atttggatca acaggaggaa ctggaacaac
    60241 gtcttcagga tttgcagcat ctggtttggt cacagaaatc gttccttctg tagtggctga
    60301 gctcttattc tcagcaaaaa cactttgtgt acataataat agacttccag ctagaacggt
    60361 acttaatgca attgtttttt tcatttcttt ttccttcttt cttttaaaat aaataatcca
    60421 gcaaataagc caataagaat gactccaaca agtatcagcc atcgccagtc aaatgtttct
    60481 ggctgatttt ttaaatcaat atccttttca ttcaattggt ccgctgcatc atcagttatt
    60541 tcaaaatcgg tatggagatt ccagcgtttt tctttccaag cacccttcac aaccgcttga
    60601 taatgtcccg gagctaattt ttttccttct aatgggacaa gtaaatcaaa atttgtattg
    60661 ggtgctacat ctattttttc tttttttatt gaaaggtaag gctcttttgt ttcttttttg
    60721 tagatttcaa ccgatgtagt caagccgaac atataagaag catttggatt ttgaagattc
    60781 gccttaatca catttcttcc atcgctttga tccgcataag ctttttttaa tttcaaatct
    60841 ggggaagact ctttttcgct atttcaaatc aagattgctt tcacttgaga aaactgattg
    60901 acgacaccaa ctgtactttt gttagaaacg ttctcttttt tttcttgaaa cgtgatccct
    60961 cctgcgacca caccagccag ctctttttct ggcgctttca gtgaaagtgt ctcagtgacc
    61021 gcactttgag gaggaacagt tatttctccc actgtttttg ctaagttttt catatcataa
    61081 cttaatgttt catcaaattt actttttgat tctccatatt cgatcacacc gtttgaattc
    61141 gtcgtagcgg agtggatcaa tgtctcaact gttactgatt tatctgttcg gttaaagacc
    61201 gtgtagggga tttcctgtgt tttcccaggc tccatcctta catttatata tgttttgttc
    61261 ttatcaatct gattagccgg taattcaaaa tctaccgcaa aattgaattc ggaggcatgg
    61321 acaggtgtct gccaaaggat gaatccaagg agaaggatca taagtaaaga gcagttgttt
    61381 cgatgacttt tattccatat tcgattcatc aatcatcctc cttgatcttt attctgtcgg
    61441 cgcatctgct aatgtccatg ttatctctgc tgagtaatta ccagctatct gattttcttt
    61501 ggttaagaca agtttaaacg cccgaccgtt atttaaatat tctgaataat ctgcttgcac
    61561 attcgttgaa ttttgtcctc tgacaatttc cagttcttct tcattcacta attgatactc
    61621 ctctcctttc ttgaaatata ggacatcctg catagaaaat cctgcatcat ttgtgaaagg
    61681 ctgagataat tttatcgtta agatccacgg atttttcgtt tctcgctgat cataaatcca
    61741 taatttttcc tgtatatcca tttggccaaa tgcttcttgt gtcaaaccag ttgctttgat
    61801 ctgaccaaaa tcgagtttgg aaggggcaag aaatgagaga ccaccttcta ctacgatctt
    61861 cgtagcaggt gcaaaaggcg gggcatcatg ataaacaact ggcgtatcac ttggattgtg
    61921 attcccaatt tcatcgttgg tacttggctt gttctgcaca cccttagcag tagcttctcc
    61981 tgcttggtcg tttaatacaa tttctaatac atctcctgca aaaagttgat tttctgggat
    62041 agaaagaaca aattcccctg tatcatcaat agttgtttct gacaaaacag cttgattttt
    62101 tttcaatgaa accaaggctc ctgcttcacc tgtacctgtg atcgtgcgag aagctgcatt
    62161 gatcaatgtg gtacttaaat tagcaggttc aggaggaatc acatcgactg tcgttttggc
    62221 agagtccagt tctgtcactt cttggccacc tcttactttt ttagcagcaa caacttgcac
    62281 ttgatcctcc gtttttaatc tctcatcatt gggtacttga acttcgaaca agccgccttg
    62341 ttcgccttct ccccatgggt tatatcctgc cccttcattt tcatctgttt gtccttgcgt
    62401 gagagcagta atttctacag atgatttcgt cgtatctgcg taagtaatac gtaaagtcac
    62461 ttctacttcg cctgtccaag catctcttgg ctttgcctcg acaccttcgg gaacaacgac
    62521 atgcccatag atttttttat ctgcattcgt tggcactctt aaggattcaa tcatcgggag
    62581 ctgattattg gcactgattc gattataatt tttcattccg gcacctgtct gagcaaattc
    62641 atttgttaaa cctgcactag ggcttgatac gaaattgcct aagtcggtcc cattgatcga
    62701 taagtctgtc atatcagaaa aataatccgg gtctttttca aagttatcga atgttgcgcc
    62761 tgtttttcgc cataaagcga tttctgaatg ggcgatcttt aaagaagaat ctccatttcc
    62821 ttggaatacc cagccgccta tgccattggt ctcgcttcca atccgtttgt tttggaaatc
    62881 aaaataggta ggagattcta agtccacatt caacacatcc ccactgatga tgccggtctt
    62941 atcttcccct gttttcccga ccatgactaa ataagttttt tcacctgccg taaaatttaa
    63001 atcaatatct ccagttgtat ctacagttgc tccggaatct gcgcgtatat cgatatttga
    63061 tttttctcca gtgactgtaa aatcagctga acctggactt tctagaaaac cagaagcgtc
    63121 ataaaattgg atcgcttgat ttccttgatt gagtctgttc acgtaagatt tgccatcacc
    63181 tgtatttctt accctcaatg agcctccatt ggttactgtc agcttttgcg taccattctc
    63241 aaaacgaact gcgctggaac gcccactctg cttatcgatg tcgataactg atccggctcc
    63301 ttctacgatc aaagaaaggc cttttcctac aaaacgcatc ccattgttcc cgggttgatc
    63361 gtcatctcca gccattttga ccgagatttg tccaccactt gatacatgga tttgactaga
    63421 atctccttgc aagcgaaatc cgcccgtagt tgctgtattg atggtgattt tcccaccatt
    63481 cgatacagtg actttcgatc ctgaacctct catttggatg attccgcgat tgttgtcatt
    63541 ggtaatattg atctttgtat ttaccgaaaa ttcgcttccc gaaccactga cttcaaacat
    63601 gacattacca ggtagcgtat cgactgcctc tccttcatcg ctaacgccat ctgcccgatc
    63661 ataatcaaga gagacctttg aaccgcttaa gatatggatt cctggcgctg tttttccact
    63721 accagtctca tctcctgaat ctagaaaacc gtagaatttc cctagatacg gtccataaag
    63781 ttgactacca ttgatcacat tcaattgata gcttctacaa aaataggctt tacccggagc
    63841 ggcattcagt ccttccttca ctggttggac atccaacaca ccggaaacac catctaatgt
    63901 gacagttgca cgaggagcgt aaatcagtcc taatttggac ccatcataca aatcaagtgt
    63961 cccggtaaat tttatttctc ccgtaaaatg atatctgctt ccccaaaaat cggtcatata
    64021 agcaatgata atggcatttc catcgatatt tggtgtcaat ccacctggat gtgcagctgt
    64081 tcctttaaaa ttctggaact cgatcgactg aggtatctca ttgatataaa tatggtgtgt
    64141 gccgaaattg tgagcgaatc caccaaaatc aataacgata tctcgtttga tctctaattc
    64201 cctgtctaaa gtcaaaggct gaatcagtcg gatcttagtt actgattcgt ctgcgagaag
    64261 ctgcgccagt tcttctgttg tggcagcttc gctgtaccca tccgctgaaa cagctcttgc
    64321 cagtgccgct tcactgctat cagacgtttg ttcagctact tctgaggtct cacttgtctt
    64381 attttcttct tgccttttat tagtagaagc ctcactttga gattctgtag tttcagaagt
    64441 aatcgattct tctgaaaaag tagtcttgtc aaaactttga ttctctgatt gcgttgattc
    64501 gtttgttttc tctacatcgg caaaaacgga tgtcaccatc aagagaggga agacaaacaa
    64561 aatcgacgca atcagctttt ttacacattg attcattctc caccatcctc tctgacgttt
    64621 tgacaatttt attttatcgt tttaatctat ttccaattgt actttcatat ttaaaatatg
    64681 tatttttatg gaaaaatttt tctattaggt gtaaatttct tttttgtaca aaaaaaaggc
    64741 tgtgacaaaa gtcttgtcac agtcccttat cccgaacaag cggtgttctc ccgccaactc
    64801 ctcggtcaca acctccattt taacaataaa cctgtaaatg tgagggcaat actttgattg
    64861 tcacaggtaa tttatcccct tcatctccat caacattcgt cgtcaattct tcctcatcag
    64921 ctaatgacac aactaattgc tcacaagtaa ggtaggctag atttttcgta gattcatcta
    64981 ctccttttaa taggtctggc aatgttttaa gcgtatcgat caaagaagaa tcttttaaat
    65041 aaaccaaatg gagcttacca tcatccactt ttgcatttgg aagtaatgtt tcaaaaccgc
    65101 cgactgagtt agttagtcct atcagcaagg tactgctctc aatttcttcc ttctttccat
    65161 caacttctac gtgaaacgag taggattgtg tggaagctaa ctgtttgatt cctgacatga
    65221 aataagctag ttttccaaat ttcgtcttct tctccgcatc tacatcattg attgattcag
    65281 ggatagagcc aattgcgacg acattcataa aatagtcgtc atttattttt ccaatatcca
    65341 atggcttgac tgattcaatc gaaaaatgat tgatcgcttc ctcaggttct agcggtatcc
    65401 ctaatgctcg agccaagtca ttcaccgtac ccaatgggaa aaatccgaaa tttggccgat
    65461 gttcttgttc agcgattcca ctgattcctt cgttgactgt tccatctccg cccatgacaa
    65521 acacactatg atacccttcc gtagccgctt cgcgagtaaa gttttttgca tcccctgctt
    65581 tttttgtatg caagacgacc acttcatcaa acaccgattc caattttgct atcgctaatt
    65641 gctcaaattc ctttgcttgt tcaccgcctg aactggggtt taccactaat aaaacttttt
    65701 tcatcacttt ctttcccttc tgattgattt ctttaatata acgatagcat aaagaaaaaa
    65761 attaggctgt gatgaagtac tgttcatgaa acattcattt attttttgac aagataaaac
    65821 atccatacta tgaaactgag gaaaattaaa acaatgatat aggaaataaa agaaaatgat
    65881 aggcattgta tacctaataa agtaaaaatc ttaggtggca gataaaaaca caaaaatagc
    65941 aagagaatgt aatagcgttt cttccacttt ttgttatcag accttatagc gatcagagga
    66001 ataccaaatg cataactgaa aaactcttga tgtactgtta atatgtcacg gccttgaata
    66061 ataaatgcga caatccccgc tataattagt gtgcctaaca ccaaaacgga aaattgatga
    66121 atatatttta tatacaaatg atccattgta cttcctgcct ttttatttgt gagaaaaaca
    66181 aatccgtata cgtgaataac ttctagaaat aaaagaaatt tgcaaaacca ctccatttat
    66241 ctacttctac gtgcattttt tgatattgtt ttctttattt aagattatac agaaaaatat
    66301 ctagggtcgc tggattctaa gtaaaaaaaa tttttaaaat aaaaagaact aaatcaagta
    66361 gtaattaaac tactgttgat ttagttctaa taaaaagatt tagatgaaca gttttattga
    66421 tttaaatagt tcatattttt tatttatacc gcttatttac tacttgctgt actagaatct
    66481 gaagtgaatg tactcaaaac attttcaaag tctttgtctt tgatttttac attagcgtct
    66541 ttcaatactt cgccaattac tttattctgg aaggtactgt cacttaattt tgtgtctgtt
    66601 gcaatttctt tcaattcttt tttgtattta tccatgtcgt tgcctttgtt ttggtttttc
    66661 accattttaa ccacataata ttccgtagta taagttgaag cattggtaga agtgatcaca
    66721 tcagaaactt gaccatcttt caacttaaag gctgcttctt tgacttctgc cggaaccgtt
    66781 gtagtagtgg aatcaaattt cacttttccg ccatcttctt ttgtcgtagt atcagttgac
    66841 ttgtctttgg ctaatttaga gaaatcgtct cccttatcag cagctttctt cacatctttt
    66901 gcatcatctt cacttgctac ttggataatt tgagcttcca cttctggatg gaaagattcc
    66961 caagccgttt ttaaatcttc atcggtcaat ttgatatgtt tctttagacc ttcttgaaag
    67021 gctagacttt gtttgatact gtctttgaat gtttgttctg tgtaacctgc agatttcaat
    67081 tgtgaatcaa atgaatcacc caattgtttc tttgtttgat catattgaga atcgatttct
    67141 ttggttgata cgtcatctcc atatttatct tcaaatactt gataaacgat caactgttgc
    67201 acagcggaat ctgctccttg catttttttc aagctatcca ttacttcttg ttcgctgatt
    67261 gtaccgcctt tataactaac ggctgttttt tcaccggcat tcgtacagcc tgacaataag
    67321 ccagtgccta gtacgatggc aagtgctaaa aatctttttt tcatttatca attctcctac
    67381 ttttcgtttt ttgcgaattg ctaaaaacaa aggtgttcaa gatgaaaatc atcaagaaca
    67441 cctttactaa tctgctcgat acgtcctgaa tagatataag gctacacaat ccagaaagtt
    67501 cgctcagcag aataaatgtc aagtagcaca tcacacctta atatactact tcttttgtta
    67561 ccgtttttca agtctatctt gcgttccgta tttaacgtaa ctaattctta gaacaaatgt
    67621 tatcgatttt gtttttcttc ttccgctgct gggatattca aatcagacac ttttatgttc
    67681 gattttccag acattttttt gttgttttta ttatgatata tttttataca actaaaaatg
    67741 aatatattaa aactaccaaa aataactttt aacgctgttt cattatatgt tttttctatt
    67801 ttatataaac ttttggatac ttaataccaa ccggttggat tgtaccaaca tgagaatgtg
    67861 tcagttctat ttcctgtcgc acttgcatag gcccatactc cttttgttgc atttgctgat
    67921 cgaatagagt ttccaacttt cgcttttgca ccgtgacgat tatagacacg actatagtgg
    67981 ttagaatgac ctgtttttct cgtacaagtt gtatcactat aataaaccca tcttccagag
    68041 ctatgattaa ttgctgttct agaaatttcc cggtctactg gtaaatttga cttaataata
    68101 ttacctttag catcaaaatg aatctctcct ggctttaaag atttgtaata gatttcaccg
    68161 tgaactgaat ttgcttccac ttcagatgac atagcgtttc ctccaacaaa acctatccct
    68221 agtgcacaaa taataactgc ttttttcata aaacgacatc ctttcctttt tgatttttaa
    68281 agcatgatct tcttgtttcc tattttggtc agatatggat catgcgtgac tacgataact
    68341 aacgtacctt gggaagcttg ttgcgataag atttcaacta ctttatcctt atttctttta
    68401 tccaatgacc cagtcggttc atcggctaaa atgattttgc tattcttcaa taagactctc
    68461 gcacaggcga ttctttgctt ctcaccacca gataatgtgt agataggcgt acttagcttc
    68521 ttgtttatat ttactttttt caatgcctca tactttgctg ttcgcttatc tgaaacattt
    68581 aaattcgaaa acttcaatcc aatatctaaa ttaaaatcga ttgtttcata ttcaatcaac
    68641 ccaaaatctt gaaaaagaaa agatatggta tttcttttta tcaagcgctt ctctctccca
    68701 gttaattgtt tcgtctcttg atcgaacaaa tagaattgtc ctgaactgaa ctgatcattc
    68761 aatcctaata tattcaataa cgtactttta cctgctccac tttctccaac aacagtcaca
    68821 agatccccag catccgcagt gaaacgatcc aaatgaagaa tcgttttgtt tcgaatgatt
    68881 tttttcgcac cttttatcac tatcaacggc tccaattgtt aacctccatt catcagcaat
    68941 aactgacttt tcttttctgt agacaaaata aaaacaactg aaagaataaa atcaatgaag
    69001 ctaaagaaaa aaatattaaa aagatattta ggctctcttt ctataagaaa aaggaagatt
    69061 acacttgtcg ttccccattt gatgaggttc atcatgaaga aatctttata cgtttcaaag
    69121 agcgaataac cacttgaacg aaagatagcg attttccgac aattgtagac aaaatatatg
    69181 cataacatga catagctcaa caagaagaaa gaaaagaaag tgagtaaaaa tatctgtatc
    69241 aagtaatgaa atgatctgct taacactttg acagctatct cttcagtctg agtatacgga
    69301 aatgataact gcaagttatc acgtaactga ctttcttcaa tcagctcaaa taacctttct
    69361 ttcgacaaag agcctatctt gatctttaac ggtggataga tttctccgct gagaatattt
    69421 ctttccaaat acatactgtt tttcaatgtc agtatttcaa caaccggata atgttcgatc
    69481 caggggatag aaggaataaa tgtataaatg ggttgattgt tttttataaa tagaaatttc
    69541 ggttcttttt cactgatttc ttgataatat tctgttattt gcgccaggtc attttttaaa
    69601 tctgtatgtc tatatcgttc agggatcaat ataacagggt gcgtttcttt ttcggttatt
    69661 tggatcaacc ttccagagac atcaatcaaa ggaaatttct taagataatt cagatttatt
    69721 tttatccctc tgccaaactg ttcattttct tcattcaagt aatctgatac attgactaat
    69781 atacttccgt ttttattcaa agtttcatat aattcctgat ctgctgctga tgcaaaacga
    69841 ttggaataaa taaaatccac gtggtttttc ccgatggtta ctggataaaa tacatgatag
    69901 tcctcaccag cgatttcttt tggtggttca tgggaaacag ctaactgtga agaagaaaaa
    69961 acgatagttg ctaattcgac attggcaacg gtcaaaagaa gagaaaacaa caatccaaat
    70021 gtaaaaagaa tgtattggat atagttgaac gcttctctta acgaagcgat tttttcaact
    70081 aataaaagag aaatcaaaga gataccatat attccgctga acaagaaaac gtacatctgg
    70141 aacaaataaa gaaattgctc cttataatac ataaaaataa aaccaccaat gattcctgca
    70201 attatgaatg taataatgat tttctttttc cccaataaac accacaatac ttgatgtaat
    70261 gaaaagtcat tattcaacaa taaactgatt tctttattca tcaataagaa aaaggatgat
    70321 aacaaaagga ataaaagaat caatccaata gttaaataca ctcgataatt atatacgtct
    70381 gtatttgaaa taaccgaata cgaatagcta ttttcaaagt cttcaaaaat attagtggca
    70441 taatcagtgt gaaatgcttg attgaatcga tcagtgaata gtgaaatgaa ccgttcatag
    70501 gcttcttttt ctctcgtcat caagaaaaat tcaccttcaa aatcttttat tttgatcgat
    70561 tcttcaatgg acgttacgcg aaaggaggta ttagaatagg gtaccttctc gtgagaagat
    70621 ttgacatccc cttgataata caatgtcatt tcaagctttg gctgtatctt gaagttccaa
    70681 gatgtatcta cttcattgga ttcatttact gtttttttca tataagaaag ctcagtttct
    70741 gatgagacat tttcaaatac catgattagc tgctcatatt cttcttgccc aataaaatgt
    70801 tgtggcaatc gaatcggagt ataagtatct gtttgatgga aaaattcatt tgtccgatag
    70861 agtacctgtt tatttatact aaccaaggca aaaccactca tgataccggt cactatgatt
    70921 gtgagtaaaa ctgtgaactt ttttttgatt atcaccactc tcccttactt aaatgattct
    70981 ttgataaaat cataccgttt ttcatcaaaa aaacgtatct atcaagtgta taggtcatgt
    71041 agaataaatc tatatactgt agaatcgaaa ggagaagaag atgaacaccg gagaaaaaat
    71101 acgaacttac cgcagaaatt gtaagcttac gcaaaaagaa ttaggcgaaa aaattggtgt
    71161 ttccgataaa acgatttcta gttgggaaaa ttctcgtaca atgccagacc ttgaaatgct
    71221 gtcactttta catcatactt taggtctgcc gattgatttt ccttctattg aatcagctac
    71281 cgaatcaata gaaccaacat tgccagatga acctcttgtt aacaagaaat cttattcatc
    71341 aaaacaaccc ttctcactca aaaagacact atctttcttt ctgctcgtct tttgtttggt
    71401 ctatcttttt cttatcaatt atcaatacaa aaatgaaacg atcgatcgat tcaatccttt
    71461 tttgaaacca caaatcggct ataccacact ccctgtagga tacgtggaca attccatcga
    71521 acgttgggta tgtaatgata tctttggcca tggtacatgg ctgtcttttc acgctggaga
    71581 ggtagaaaaa gagaaaaacc atgctgtaat caaacacaaa ggaaaacacg tcatgaacgt
    71641 tcgcctgctc acttgggaag ccattccctc agattaccaa gaaatcctca gccaagacta
    71701 tgacgacgcc ttaaaaagaa gccctcagga gccagatagg cattaagtag gagatgaacg
    71761 tagagataga aaaaatcaat gcacatgatc ttatccaaaa aacaggggta caaattaatt
    71821 gaaaaagcct tgagatgaaa gttcctaaat aaaaaagttc agaatgatag gtatttctgg
    71881 cttatagtat aatatttacg ccacagcctc ccaatgattc tttttggaat cataatcaag
    71941 gatggacatt cacaaaaata ttttgctcat tcgatccgaa tagattaacg accaatctag
    72001 taagtaatat agattggtac gtagtcaagc tgcacataat ttagatcaat tttagttctt
    72061 ttttatattt ctatgatctg ctcatttact tttatatgat ggcttacagt aacataaata
    72121 aatatgataa tcgctggtag tttatccaag agtactcaat aaaatacaaa atggattgtt
    72181 caagcgccta tcataatgat aaaatgaaac aaataaacga tacaaggagg aagagacttt
    72241 gagtaatggc agaacaattt tttcattgta aaaattgttc agttgttaat ctatgggatt
    72301 tcggtagcat gggcattttt ttatagagga atcacaccaa cctggccgtt ttatctcttg
    72361 ttagtgggaa tattagtagg aaattttgcc cctcttcact atcgtacgaa cttttttcca
    72421 actgattctg gcctttttct taaaacaaag acgcgaaaat tagaaaacat tattgaaatg
    72481 acaattacct gtataatcat tttcttagtt atattcctaa acgtcttcta ataagaatct
    72541 cttaaaatta gcttgttaca gttagttacc tagtttatat aacacagtta tcttatcttc
    72601 cttatcaaca tcttttgcca actctttgtc ttaaattcga aacatataag cagagctacg
    72661 aacaccaaga ctgtttcggc tctgtttttt gcatcaaatt cactcttata tagctgataa
    72721 tctcggctct ttaaaagaaa ttattttcgc ttcattttat tataaaaagg ctaggaacct
    72781 tgatgttatc aggttcctag cctttattta agtattaccc cacagaccct tccatataga
    72841 tttttaaatt acacactagt ttaaactaaa acaaacaagg aaacacttac catttaatca
    72901 taaaattagc tcatatgaat atattagaat gtaaaataga ctggtcaaaa tctggtcaaa
    72961 aaagaggact aatacaggta gtcctcagac tgtagacaaa gtccatttta tggatgatat
    73021 ctacagtctt ttcttttata atagatttat gaaaacgaat gcaaggagtg atagctatga
    73081 tgtctaaaaa caatacgaat ggtagaaatc aatttgcgat gcttacgatt gatgatctag
    73141 ttcctcaaga tcatctcgtt cgaaagattg atgccgcgct tgattttgaa tttatttatc
    73201 caatcgtcga agccacttac tccgatttag gtcgcccaag tatcgatcca gtgattctga
    73261 tcaaattggt gtttattcag tatttattcg gtatccgttc catgcgacaa accattaagg
    73321 aagtcgatac caatgtcgct taccgctggt tcttaggcta ctccttcgag gagaaaatcc
    73381 cccatttctc aacttttgga aagaattatg ttcgccgatt tagagaaacg acggtgtttg
    73441 aggatatttt tgcctatatt ttagaacaag cagttaaagc aggttttgta acagaggata
    73501 atttgtatc
//
LOCUS       DASHHB010000007        71194 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.007, whole genome shotgun sequence.
ACCESSION   DASHHB010000007 DASHHB010000000
VERSION     DASHHB010000007.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 71194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 71194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..71194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.007"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(40..1053)
                     /locus_tag="U2K71_000514"
     CDS             complement(40..1053)
                     /locus_tag="U2K71_000514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM7314625.1"
                     /translation="MKKKILKFIGWFFGIILGIAFAIFLAFQLSPRPGAFLINHMFSN
                     TVQITDKKTFDQAEKNVAKKTDLTYESKYKENTYDIYYPKNSKGPVPVLFWVHGGGFV
                     GGDKSGVKEFATKLVSDANIAVVAMNYELAPDSEYPNQVLQVNELIKDLLAEEKDDKL
                     LDMEQLFFGGDSAGSQIALQYAAIQTNDAYAKQMKIKQLLPKDSLKGTISYCGPVDLK
                     LTAQQHSDDRFMKFFVKTVAWSLIGTKDWKTSEQLKEASVADHVSKEFPPTYITDGNA
                     YSFQEQGQALENRLTELKVPVQSLFYTDTKKEITHEYQFDYTLKESQNCYQQTLDFVN
                     KYK"
     gene            complement(1131..1319)
                     /locus_tag="U2K71_000515"
     CDS             complement(1131..1319)
                     /locus_tag="U2K71_000515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HEM7314626.1"
                     /translation="MEEHQQVIDRIYEATRYLPLDRLCLSPQCGFASTEEENVLFEEQ
                     WKKFLLSKALQKKFGENH"
     gene            complement(1743..1871)
                     /locus_tag="U2K71_000516"
     CDS             complement(1743..1871)
                     /locus_tag="U2K71_000516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HEM7314627.1"
                     /translation="MDQKEKMKELIKKKQGGGRSKQMETPKNDRKNMRKGPKIFNK"
     gene            complement(1874..3247)
                     /gene="rlmD"
                     /locus_tag="U2K71_000517"
     CDS             complement(1874..3247)
                     /gene="rlmD"
                     /locus_tag="U2K71_000517"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEM7314628.1"
                     /translation="MTEQQIVKKNERYTVEIVDLSYEGMGVAKIDGYPLFIENALPGE
                     EVEILVVKAGNKFGYGKVETFITQSPDRQPVENNALLRIGIAPLAHLKYEQQLVFKQK
                     QVEHVMAKIAKMPEVPVLPTVGMAEPFGYRNKAQIPVRRIDGQLATGFYKKNSHDLVP
                     IEDFYIQDPVIDQAIKVVRDILQRFQVRGYNEEKNEGQLRHIIIRRGHYSHEMMVVLV
                     TRKEKFFKSKEIAEAIHEELPEVVSVIQNINEEKTNVILGDKEKVLYGRDYIEDQLLG
                     KTYRISAKSFYQVNTQQAEYLYQTAIEFAELDKKDIVIDAYSGIGTIGLSLADRVDKV
                     YGMEVIPEAIEDAQFNALNNKIENVHYEVGKAEKVMKKWQEKGIKPSVIVVDPPRKGL
                     DHHFIESAIEMSPERIVYISCNPATFARDAKLFAEAGYAVKKVQPVDLFPQTHHIELV
                     ASFVKGE"
     gene            complement(3358..4413)
                     /locus_tag="U2K71_000518"
     CDS             complement(3358..4413)
                     /locus_tag="U2K71_000518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEM7314629.1"
                     /translation="MKKARVIYNPTSGKELLKKNLADILQALEEAGFEASAYATTPEP
                     DSAKNEACRVAELGFDLVVAAGGDGTINEVVNGIAPLEHRPKMAIIPAGTTNDYARAL
                     KIPRDNIKAAAEVIKKHQTVKMDIGLSDNSYFINIAAGGYLTELTYEVPSELKSIFGY
                     LAYLAKGAEMLPRVKPIKMRLKYDEGEYIGNASMFFLGLTNSVGGFEQIAPDAKLDDG
                     KFSLIIVKTANIFEILHIAALMLNGGRHVDDPRIIYTKTSSLFVETEDDINRPVMINL
                     DGEYGGEAPMHFQNLHQHIEFFANTDQIPDEAITGTDEEELEVVSKEFVKEVERLTDE
                     DIDGDGKIADETPNQKN"
     gene            complement(4423..5853)
                     /gene="gatB"
                     /locus_tag="U2K71_000519"
     CDS             complement(4423..5853)
                     /gene="gatB"
                     /locus_tag="U2K71_000519"
                     /EC_number="6.3.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HEM7314630.1"
                     /translation="MNFETVIGLEVHVELKTNSKIFSPAPAHFGAEPNSNTNVIDWGY
                     PGVLPVMNKAALEFGMKAALALNCEISKDTHFDRKNYFYPDNPKAYQISQFDQPIGHD
                     GWIEIEVEGKKKKIRIERVHLEEDAGKNMHGIGGYSYVDLNRQGTPLIEIVSEADMRS
                     PEEAYAYLEALRSIIQFTEVSDVKMEEGSMRCDANISLRPYGQEEFGTKAELKNLNSL
                     SFVKKGLAFEEKRQAKVLLSGGEIQQETRRFDETTNKTVLMRVKEGSSDYRYFPEPDI
                     PKFVIDDEWIQKVKASLPEMPASRRERYIREFGLPEYDAMVLTLTKEMSDFFEAVLEN
                     GADAKQASNWLMGEVSAYLNSEKLELAETKLTPENLAGMIRLIADGTISSKIAKKVFR
                     ELITNGGEAQEVVEKNGWVQLSDPNKLLPIINEILDNNQQSVDDFKNGKDRAVGFLVG
                     QIMKATKGQANPGVVNKLLKDELAKR"
     gene            complement(5850..7322)
                     /gene="gatA"
                     /locus_tag="U2K71_000520"
     CDS             complement(5850..7322)
                     /gene="gatA"
                     /locus_tag="U2K71_000520"
                     /EC_number="6.3.5.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HEM7314631.1"
                     /translation="MTELHNRSLEELHHMLVSKEITVQDLTKATFERIKETEPEVEAF
                     ITLNEEKALEQAKALDEKGIDESNVLAGIPIGIKDNIVTKDILTTAASKILYNFEPIY
                     DATVMDKIYQADMIPVGKLNMDEFAMGGSTETSYFKKTKNAWDPTKVPGGSSGGSAAA
                     VAAGQIPVSLGSDTGGSIRQPAAFNGIVGMKPTYGRVSRFGLIAFASSLDQIGPMTRT
                     VKDNALALTAISGYDEKDGTSSGVSVPDFAEGLTGDIKGMKIALPKEYLGEGVAPGVK
                     EAVLKAAETFRSLGAVVEEVSLPHSKYGVAVYYIIASSEASSNLQRFDGIRYGYRSEN
                     VKSLDDVYVNSRSEGFGEEVKRRIMLGTFSLSAGYYDAYFKKAGQVRTLIKRDFEKVF
                     ADYDLIIGPSSPTVAFGIGENINDPITMYMSDILTIPVNLAGLPGMSVPAGLSEGLPV
                     GLQIIGNYFDEKTMYQAAYAFEQATEFHKQQPAILGGKDE"
     gene            complement(7322..7627)
                     /gene="gatC"
                     /locus_tag="U2K71_000521"
     CDS             complement(7322..7627)
                     /gene="gatC"
                     /locus_tag="U2K71_000521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatC"
                     /protein_id="HEM7314632.1"
                     /translation="MAISEEQVKHVAKLAKLAFADEELASFTDQLGKIIDMVEMLEEV
                     DTEGVPFTSNVVETVNVMRQDRAQAGWNRDELLKNVPETEDGFIKVPAIIDNGEAGA"
     gene            complement(7733..9769)
                     /gene="ligA"
                     /locus_tag="U2K71_000522"
     CDS             complement(7733..9769)
                     /gene="ligA"
                     /locus_tag="U2K71_000522"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HEM7314633.1"
                     /translation="MEHEMTIDEAAKRAEELRTRLNQWSREYYVEDKPTVEDYVYDKE
                     YAELVAIEEQYPDLITSDSPTQRVGGKVLEGFEKVTHDIPLYSLNDVFSKEELIAFDQ
                     RVQKAVGRVVDYCCELKIDGLSVSLRYEDGNFVRGATRGDGTVGENITENLKTVRSVP
                     IKLKEPMNIEVRGECFMPKRSFVQLNQDREAEGKDIFANPRNAAAGSLRQLDSKITAK
                     RNLDTFLYTVADFGPMQAKTQYDALEELEKIGFHTNREKRLCHSIDEVWSYIEEYHDK
                     RVDLPYEIDGIVIKVNEFSLQDQLGFTVKAPRWAAAYKFPPEEVETLIENIEWTVGRT
                     GVVTPTAIMTPVRVAGTTVSRASLHNGDYIKLKDIRLKDTVLIYKAGDIIPEVSQVVL
                     DKRPKDSEEYQLPTHCPVCGSELVHLDEEVALRCINPKCPAQMKEGLNHFVSRNAMNI
                     DGLGPRVLEQMYDKKLVADVADLYKLTEEELLTLDKIKEKSANNILTAIDNSKDNSVE
                     RLIFGLGIRHVGAKAAKILAEHFGDLETLSKSDYESIIALDTIGDIIADSVVTYFSNE
                     EVHELMNELKQAGVNFEYKGLRNAQLQEVESPFKEKTVVLTGKLTRFTREEAKETIEN
                     LGGKVTGSVSKKTDIVVAGEDAGSKLTKAQELGIEVWTEDQMADALAKSRSVEE"
     gene            complement(9786..12020)
                     /gene="pcrA"
                     /locus_tag="U2K71_000523"
     CDS             complement(9786..12020)
                     /gene="pcrA"
                     /locus_tag="U2K71_000523"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase PcrA"
                     /protein_id="HEM7314634.1"
                     /translation="MSSKAELLNGMNPRQKEAVLHTDGPLLLMAGAGSGKTRVLTHRI
                     AYLIEEKEVNPWNILAITFTNKAAKEMKERVNAILASGGEDVWVSTFHSMCVRILRRD
                     VDFIGYNRNFTIIDSSEQLTLMKRILKELNIDPKKYDPRSILGTISQAKNSLQTPQDF
                     AKMQGSYYEEIAAKCYAAYQKELQYNQCMDFDDLIMNTIRLFEEHPDSLTYYQNKFHY
                     IHVDEYQDTNHAQYTLVNLLAGRFRNLCVVGDADQSIYGWRGADMQNILDFEKDYPDA
                     AVILLEQNYRSTKNILSAANQVIENNSNRKPKNLWTENKEGNKITYYRADNERDETRF
                     IVDRMQEEIRSNHRNYGDFAILYRTNAQSRVMEETLLKANIPYKMVGGHKFYDRKEIK
                     DILAYLNVLANPQDSISFERIVNSPKRGIGPGSIEKLRSFASLHEWPLLEAAQNVDLA
                     NIGGKAGQQLGAFGEMIQEVTQMIPYLTVTELTKEVLDRSGYLEDLKIQNTLEAQARI
                     ENLEEFLTVTQEFDKQFEQQNEEDADAPEEKLTVFLNDLALVSDIDNLEEDASQVTLM
                     TLHAAKGLEFPVVFLIGLEEGVFPLSRALMEESELEEERRLAYVGITRAEEALYLTNA
                     FSRTLYGRTQYNRPSRFVEEIDQELLEIEGMRPTPKKTPVFAKKTAYSYKQPETAVVP
                     SKSATGGENNSWKPGDKVKHKKWGLGTVVRVSGTSKDLELDVAFPSQGVKRLLAAFAP
                     IEKA"
     gene            12175..13734
                     /locus_tag="U2K71_000524"
     CDS             12175..13734
                     /locus_tag="U2K71_000524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClC family H(+)/Cl(-) exchange transporter"
                     /protein_id="HEM7314635.1"
                     /translation="MEKKGKIKKLDKTQIYFILKGIFVGLLTGIVVSLFRLSIEKLSD
                     LVRSIYEMSREQPIYLIGLAICCILAAFFVGYLVKNEPDIKGSGIPQVEGQLRGELSM
                     NWFSVLWKKFIGGVLSVGAGLFLGREGPSIQLGASIGQGAGQLFRSPSSEEKILISSG
                     ASSGLAAAFNAPIAGLLFVLEEIHHSFSPLVWLTSFASAITANFVSLYFFGLRPVLYL
                     GDLPSLPLKYYGSLVILGVLLGILGFIYQKVLLALPKFYNRLPLPAYFHGFVPFLLIL
                     PIGYLWPSTLGGGNQIVLAFGHASLPIIMIIGLFVLRFVFSMISYGGGLPGGIFLPIL
                     TLGALIGSFYGNIMVDFGMDPIYIKDFVVIAMAGYFTAIGKAPLTAIILVTEMVGSLN
                     HLMPLGLAALVAYIVNDLLGGNPIYESLLERLLGNTQTASLKGARTTFDFPVTAESSL
                     DGLMVRDFNWPKNMLLISIRRGNQEILTHGDTIMNVGDILVILTDEANLPRIKQEIEA
                     KSSITNLKQSF"
     gene            complement(13845..15776)
                     /locus_tag="U2K71_000525"
     CDS             complement(13845..15776)
                     /locus_tag="U2K71_000525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM7314636.1"
                     /translation="MNIKDLMVKEAMIMDLQATDKKGAIDEMVQKMFDAGRISDIETY
                     KEGILAREAQTSTGLGDGIAMPHAKNSAVKEATVLFAKSQKGVDYEALDGQPTYLFFM
                     IAAPEGANDTHLQALAALSRLLIDADFVGKLKEANTPEEVQDLFQTAEEQKEAEEKAE
                     QETQAAEASAHPERKFVVAVTACPTGIAHTYMAEDALKKKAKEMGVEIKVETNGSEGI
                     KNRLTAEDIARAEGVIVAADKKVEMNRFDGKELINRPVSDGIRKPEELINLALSGTAP
                     VFHGDGKESSSEEGNADGTIGQRIYKDLMNGVSHMLPFVIGGGIAIALSFMIDQFIGV
                     PHDQLSNLGNYNQAASWFNQIGQAAFGFMLPVLAGFIASSIGDRPGLIVGFAAGALAN
                     TGGAGFLGALIGGFLAGYVIVFLRKLFKNLPKSLDGIKTILFYPVFGLLITGFLMLLV
                     NVPMKAINDALNGFLTSMSGSNAALLGALLAGMMAADLGGPINKAAYVFGTATLATTV
                     ATGGSVVMASVMAGGMVPPLAIFVATRLFKNKFSKTDQDAGLTNIVMGLSFVTEGSIP
                     FAAADPIRAIPSFIIGSALTGGLVGAFGIKLLAPHGGIFVVFLLSHPVMYLVFIAIGA
                     VVSGVIYGLLRKAPAAAEA"
     gene            complement(15794..16708)
                     /gene="pfkB"
                     /locus_tag="U2K71_000526"
     CDS             complement(15794..16708)
                     /gene="pfkB"
                     /locus_tag="U2K71_000526"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEM7314637.1"
                     /translation="MIYTVTLNPSIDYIVRVDGLKLGDLNRMKEDFKLPGGKGINVSR
                     ILKRIDSQSTALGFLGGFTGEFISKWLEKEQIQSLFTTVKEDTRINIKLKSDTETEIN
                     GLGPSISDEEIDELKQALGTVGQGDIVVLSGSAPASLRKGFYQELIDIIREKGADFVI
                     DTTGDDLKNALVKKPLLIKPNNHELAELYNTTFQSVEEIIPYGKKLLAEGAQNVLISM
                     AGDGALLFTEQGTYKTNVLVRPLKNSVGAGDSMIAGFIGAYSRTKDPIEAFKWGVACG
                     SATAFSDDLAARELIDELLPEVEITKID"
     gene            complement(16705..17457)
                     /locus_tag="U2K71_000527"
     CDS             complement(16705..17457)
                     /locus_tag="U2K71_000527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM7314638.1"
                     /translation="MLTEERRQKILDLLEQQKIVKSQDLVNLFNASESTIRRDLQELE
                     NSGFLERVHGGAKKEQLLNFEQNMSEKSLKNVHEKQQIAKSAAETINDEDVIYLDAGS
                     TTLEMIPFLQGKQITVVTNSVQHAANLVDLELATIILGGTIKLATNAVLGSNAIEQLK
                     NFHFNKAFMGMNGAHLEQGFTTPDPEEAAVKRLAMKNSEETFVLIDHTKFNQLSFTSV
                     APLEAATIITERCPLEFVNEFREKTTIKEAIQ"
     gene            complement(17616..17825)
                     /locus_tag="U2K71_000528"
     CDS             complement(17616..17825)
                     /locus_tag="U2K71_000528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314639.1"
                     /translation="MDKQLYYRIMVPASLLSVCLFFVPNFPIRSVIGFLVMFGGMGLY
                     YYLLHKERKPASVYMKKTRSENKKR"
     gene            complement(17836..19164)
                     /locus_tag="U2K71_000529"
     CDS             complement(17836..19164)
                     /locus_tag="U2K71_000529"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314640.1"
                     /translation="MIGEDKLLMTIQLTDLNEEEKKQEISDWAILTRLLIQPTFIRSF
                     TQQADPYDLRKLQEKIMLASVRDESWLVVGNDENECSFRLEDNQLLIKNVLSISVFKE
                     NQFLIRDFIQKKMIAHGVFAYMRAYSEFIYHNTKQISQRLLFEKKDEIEHLPKMKQQN
                     GEVVVDCNQFPGYDLFYQGLCFTSCWEMYYSRYYHQIIPKPIFLDIQQVEKVKELENE
                     VICIQLYRDPFNWQKPNNQMFQSYFRDQLGFDHLAWDNGVGLLKPPFVEYIYTDHTIQ
                     SVQYQNAQMQPVPKKNASFFVTKSYDIQQGDYKERRVRGTLNAQAYFPWVDENRSRMM
                     CYKVIDPTVALDNGIEAYCYYIREYLEVEVTDEKYQEYLLSLRIYVPSESLSELPLKE
                     IKHRLSDVTFKRIKKKRRSIQFDVKKGEQHLRVQFLDYRELEQLITLQKI"
     gene            19300..19455
                     /locus_tag="U2K71_000530"
     CDS             19300..19455
                     /locus_tag="U2K71_000530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314641.1"
                     /translation="MGLKFERTDNLDKLFESFAVDPEKTVAPKEEIDRFLKPQPQNEK
                     KKDKKQK"
     gene            complement(19529..20116)
                     /locus_tag="U2K71_000531"
     CDS             complement(19529..20116)
                     /locus_tag="U2K71_000531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihA family ribosome biogenesis GTP-binding
                     protein"
                     /protein_id="HEM7314642.1"
                     /translation="MKVHQAEIIISAVAPKQYPDTQLPEIALAGRSNVGKSSFINTLI
                     DRKNLARTSGKPGKTQTLNFYLIENALHFVDVPGYGYAKVSKTERAKWGKMIETYLTT
                     RDQLRAVVSLVDLRHKPSQEDIQMYQFLKYYDIPVIVVATKADKIPRGKWNKHESEIK
                     KALDFDRNDTFILFSSVTKQGKEEAWAAIEQAIES"
     gene            complement(20199..21449)
                     /gene="clpX"
                     /locus_tag="U2K71_000532"
     CDS             complement(20199..21449)
                     /gene="clpX"
                     /locus_tag="U2K71_000532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpX"
                     /protein_id="HEM7314643.1"
                     /translation="MYDNPSSNETVRCSFCGKTQEEVKKIVAGPGVYICNECIDLCKE
                     IIDEEFYEEAVREFTEVPKPLEILEVLNNYVIGQDRAKRSLAVAVYNHYKRVNQQAQE
                     NTDDVELQKSNICLIGPTGSGKTFLAQTLAKTLNVPFAIADATSLTEAGYVGEDVENI
                     LLKLLQSADYNVERAEKGIIYIDEIDKIARKSENVSITRDVSGEGVQQALLKILEGTV
                     ASVPPQGGRKHPHQEFIQIDTTNILFIVGGAFDGIETIVKNRMGEKTIGFGTQNQKLS
                     EDESVMQHIIPEDLLKFGLIPEFIGRLPVMAALEKLTTDDLVRILTEPKNALVKQYQK
                     LLSLDNTELEFEPEALRAIAKKAIERNTGARGLRSIIEEIMMDVMFDIPSNENIEKVI
                     ITKEAAELSGEPTVIYKDSEKKAG"
     gene            complement(21696..22985)
                     /locus_tag="U2K71_000533"
     CDS             complement(21696..22985)
                     /locus_tag="U2K71_000533"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HEM7314644.1"
                     /translation="MTATFEKKGTNDGVLKFTIAQDEIQKGLTTAFNKVKKSLNVPGF
                     RKGKVSRQVFNRMYGEEALYEDALNAVLPEAYEAAVKEAGIDPVSQPKIDVESMEKGQ
                     DWVITAEVTVKPEVKLGQYKDLVVEKQDREVTDADVDARIKREQEAQAELVIKEDAAA
                     ENGDTVVIDFEGFVDGEAFDGGKGENYSLELGSGSFIPGFEDQLVGHKSGESLDVNVT
                     FPEDYQAEDLAGKEAVFKVTIHEVKAKEVPELDDEFAKDVDDEVSTLDELKEKYRKEL
                     VETKEKAADDAKDEAAIRMAVENAEIVELPHVMVHDEVHRSMDEFLNNMQRQGISPEM
                     YYQLTGSTEEDLHKQFEGEAEMRTRTNLVIEAIAAAENLQATEEEINAEIQELSETYN
                     MPIEQIKRVLTEDMLEHDVTMKKAIELITGTATEAKE"
     gene            complement(23139..24071)
                     /locus_tag="U2K71_000534"
     CDS             complement(23139..24071)
                     /locus_tag="U2K71_000534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314645.1"
                     /translation="MKPIDFPEKYENLMRVAQQALANQQYQQAKELFQRAYELKESFE
                     ANSLLVFCLYELDEKKEALKQALLHEKQYLQNEEFAEFYFDLLISTQDFLYARKLIAS
                     TDFYEAFEQRVIEKIQFAEELSGQMERQKIKALHNKSEELPSLEPARQLASIDEIEQL
                     PYHEFIQTASKLIVSPEVHILARAKLLETLRQLNECHPVFYLTIEEKLVKVIPKDLPK
                     PQQQASYQQLCVFSDRYGNEDALLSSVLNEEFTLQSAIVYPVYDTYIEDPKRWFQLTV
                     EACTGKTMYEGTEDEKQDFLKKREKILQQMIFFH"
     gene            complement(24228..24887)
                     /locus_tag="U2K71_000535"
     CDS             complement(24228..24887)
                     /locus_tag="U2K71_000535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEM7314646.1"
                     /translation="MSELSNENKEQEKDKQQQPAVGPDSNFSQKVLSSFEREPLTPEE
                     IKKKQRKWKKYTDTRNEPPKANDFPKYFYAGFFIRMFAFLVDLLCIGAITRLTLGIAK
                     NLGWLNFSDSYLSIYGFTALLIYLAYFILLTKFNHGQTIGKMIFGIRVISFDEEELSW
                     STVLVREGACRFILKFPLLMLGYLPAIFNQKKQHLGDFFTNTSVVTINLIKAFNQEVN
                     S"
     gene            complement(24890..25915)
                     /gene="sppA"
                     /locus_tag="U2K71_000536"
     CDS             complement(24890..25915)
                     /gene="sppA"
                     /locus_tag="U2K71_000536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HEM7314647.1"
                     /translation="MNKRRWAAVLIAAGLFVVSLVSASLTKPQENDQIEGLNSWLYGD
                     EELASNVLEEGDSTQKIVKLSVDGTIAAGGSSGLFASEGYNHKNFMEQLKAIEEDPTV
                     KGIFLEVNSPGGGVYESAEIAKKLDTIRKEHDIPMYVSMKNMAASGGYYISAQADKIF
                     ATEETVTGSIGVIMSGLNYSGLLEKLGVEDTTVKSGALKDMGSGTRPETKEEEAVLQA
                     YIDSAYSRFVAIVSEGRNKSEEEVKKIADGRIYDGVQAKEVGLVDELGFPEDALKAMR
                     KEQQLEDAELVEYSSTPSGFASTWLGSKLAEFQGLKPSETSQILSVLENLGTAESPKA
                     MYYYGGE"
     gene            complement(25928..26218)
                     /locus_tag="U2K71_000537"
     CDS             complement(25928..26218)
                     /locus_tag="U2K71_000537"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314648.1"
                     /translation="MDSKIEIMTLGMLKKQLSKFEASAGVSDDTKIFLDTGWDSIQEI
                     APDALEVAEAREFTVEDELTKESFSGYAREEKAERFDTSEQSETVIVIKNLY"
     gene            26628..28433
                     /gene="glmS"
                     /locus_tag="U2K71_000538"
     CDS             26628..28433
                     /gene="glmS"
                     /locus_tag="U2K71_000538"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HEM7314649.1"
                     /translation="MCGIVGMIGLKNVTPGLIDGLEKLEYRGYDSAGIFVSDGTTDYL
                     VKAQGRIQNLKNKINIDTTGNIGIGHTRWATHGQPSEENAHPHTSQSGRFVLVHNGVI
                     ENFEELKIAYLANDHFIGETDTEIIAHLIETFAKDTTTQEAFLKALRVIKGSYAFALI
                     DRTAPDVIYVAKNKSPLLIGLGDGFNVIASDAMAMLAHTKEFVEIEDEEMVTVTSEKV
                     IIQNFAGDIVERSSFEAQVDSSDIEKGTYPFYMLKEIDEQPIIMRRIAQKYVTEDNRV
                     SLDQKLVDTLSDSDRIYIVACGTSYHAGLAGKQTLEKLTQIPVEVHLASEFGYNTPLL
                     SKKPFFIFLSQSGETADSRQVLVKINRLGYPSLTITNVAGSTLSREASFTLLLHAGPE
                     IAVASTKAYTAQIAVLTLLAKAIGDKKEFETSLVFDITHELSLVANAMESVIAQKDYL
                     EELAEVYLSDTRNAFYIGRGADYNVSLEAALKLKEISYIQAEGFAAGELKHGTIALIE
                     EGTPVIGIISEEVTGAHTRGNLKEVESRGAKTLVIVSEGLEKESDQLVLPTVHPYLTT
                     LTTVVPTQLLAYYATLLRGFDVDKPRNLAKSVTVE"
     gene            complement(28505..29860)
                     /locus_tag="U2K71_000539"
     CDS             complement(28505..29860)
                     /locus_tag="U2K71_000539"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010739004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HEM7314650.1"
                     /translation="MGKYFGTDGVRGEANKELTPELAFKLGRCGGYVLSQHEEEGKRP
                     RVLVGRDTRISGQMLEEALISGLLSVGIEVFQLGVISTPGVAYLTRLQKASAGVMISA
                     SHNPAQDNGIKFFGADGFKLVDDQELEIEALLDAEVDELPRPSAEGLGTVEEFPEGLL
                     KYSQFLVQTINGDLSGLTICVDAANGATSTSVNRLFADLETDFYTMGTNPDGLNINDG
                     VGSTHPERLAEMVVEKGADAGLAFDGDGDRIIAVDELGNIVDGDKIMFICAKYLAEKN
                     RLKKDTIVTTVMSNLGFHKAVEGIGLKDVVTQVGDRYVVEEMRKNDYNFGGEQSGHMI
                     FLDFNTTGDGMLSGIQLLNIMKQTGKKLSELAAEVTIYPQKLVNIRVSDKNGAMDVPA
                     IKKVIEEAEAEMGSEGRILVRPSGTEPLLRVMAEAPTDEKVNYYVDKIAAVVKEEIGL
                     D"
     gene            complement(29884..31053)
                     /locus_tag="U2K71_000540"
     CDS             complement(29884..31053)
                     /locus_tag="U2K71_000540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314651.1"
                     /translation="MISKEKRTSILYSFIALLFSIALYFNANGQSVPNTLSGNEAYNQ
                     TVTGVPIKISYDSKRYYIHGYENAVTVKLSSANRVQLNAEANEDTRMFRVTADITKLG
                     EGTHEVPLKIQNLSSAVTAKIEPSTITVTVEKKVIKDFDVETLIPSTFTPSGYELDDT
                     SVSPKKVSITTGDKTLAEIKRVVADVDASMVTDDGINSEVPIQALNAAGEVLSIVSDP
                     VQVTVRADSIKPSKSVRLYGIQQGTPAAGVESYDFSFSKLEAEVSGSSDLLASIGDSI
                     AVPINVSGINHRTTRKIEIPVEEGLSIKPESVSVEITPVLQTSSSSSASTSSRTQENT
                     STSRSISSADSGTEPSTTTISSSSESGTSSSTTSTEETSETSESTQMSRTNSSEN"
     gene            complement(31050..31937)
                     /locus_tag="U2K71_000541"
     CDS             complement(31050..31937)
                     /locus_tag="U2K71_000541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00159 family protein"
                     /protein_id="HEM7314652.1"
                     /translation="MSFQIGELFNLDYWQQMFSGDLFSLNFLVNILDILVVWYLVYKL
                     IQLLRGTKAIQLLKGVAIFIVIRIVAEIIGLHTLSWLMNQVITYGVIAAIVIFQPEIR
                     RGLEHLGRSSLFKQSKSEQQEDEKMILSFDKAIQYMSKRKIGALITIERHTGLDEYIE
                     TGIALDADITGELLINIFIPNTPLHDGAVIVKEGKIAVASAYLPLSESMLIPKEFGTR
                     HRAAVGISEVSDAITIVVSEETGDVSITLDNELMAGLSQQEYLAILRRELIAEEKTKN
                     KKNVIQSFLDGMTKGGDRK"
     gene            complement(32126..35806)
                     /gene="nifJ"
                     /locus_tag="U2K71_000542"
     CDS             complement(32126..35806)
                     /gene="nifJ"
                     /locus_tag="U2K71_000542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HEM7314653.1"
                     /translation="MRKMKTMDGNTAAAYISYAFTEIAAIYPITPSSTMAELVDQWSA
                     AGKENVFGQPVKVVEMQSEAGAAGVVHGSLKTGALTTTYTASQGLLLMIPNMYKIAGE
                     LLPSVFHVASRAVTTNALNIFGDHGDVMAARQTGFAMLAESSVQEVMDLAPVAHLASI
                     EGEVPFLNFFDGFRTSHEIQKIEVLEYEELAPLINQEKLASFRKRGMNPNHPTVSGTN
                     QNPDIHFQQRETINPYYDKLPEIVQKYMKEINRLRGTSYDLVTYYGADDAEEVIVSMG
                     SVAQTIEQTIDYLNANGRKTGFLNIHLYRPFPVETFVQKLPHTVKTIAVLDRSKEPGA
                     GGEPLLLDVQSALYDSELRPAVIGGRYGLGSKDVTPDQISAVFDELKKDPSIRKKRFT
                     IGIVDDVTYQSLEPKESLDLTEPQTFQAKFWGFGSDGTVGANKSAIKIIGDHTDKYAQ
                     GYFYYDSKKSGGLTVSHLRFGDTPIRSAYLVEHADLVACHTPAYLHSYDLVKGLKPGG
                     IFLLNTLWSDEQLETHLPLKLKRYLAENNIRFYTINAMRLAQEVGLGRRINTAMETAF
                     FKLADIIPFDEVLPLLKEEALKSYGHKSMAIVEKNIQAIDRTTELLHQVAIPDHWRTL
                     EMPEKINKRNTGYIQEILEPVNAQEGNQLSVGTLVANGMTTGEMPLGTASMEKRGVAL
                     EVPEWISDRCTMCNECAFVCPHAAIRPFLADEEEMEEAPEGFIVREMRGADGVKYRIQ
                     VSVEDCTGCGLCVEACPAKEKALVMKPYEEQKEQAVNWAFAMTLRQKANPVKGKNTVL
                     STQFNQPLLEFSGACSGCGETPYVKLLTQMFGDRMMIANATGCSSIWGGASPASPYTT
                     NECGQGPAWSNSLLEDNAEFGYGMLLAAQTRREALASSIKEVLPKVSEELRELMEDWL
                     VHMFESEGTQQRAAKLQAAIESERSIVPELEDIYKRKDLFVKNSQWMIGGDGWAYDIG
                     FGGIDHVLASGADVNILVLDNEVYSNTGGQTSKATPASAIAKFSASGKYASKKDLGMM
                     AMTYGNVYVAQIASGANQMQTIKAFEEAEKYPGPSLIIAYTPCITHGLLGGMKESLKE
                     AKEAVSSGYWSLYRYNPAMVEKNKNPMTLDYKKPDFSAMPDFMRKQVRFSSLENANPN
                     LAKALFEKTIQDAKTRFYNYAALTGELEKIKKRLEPEESIDVPVKTRKEKTTDLEAEE
                     RRAARAERRKKMEQATKEKKEK"
     gene            36004..36525
                     /locus_tag="U2K71_000543"
     CDS             36004..36525
                     /locus_tag="U2K71_000543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEM7314654.1"
                     /translation="MDISVIDATKTNTQKGILYGSSNAPKKMVEFINLSCPYCRQWFE
                     ESYELLEEAVQSGQLQRVIKLFDKEKESLQRGNVMHRYLTISDGQKAIKEIKQIFDTQ
                     DEWKHLSLQEVADFAVDKLKLTEQKDEQLSQAVINEAEQAHIRFVPTVILGKEIFDES
                     ISIEELKELIQTK"
     gene            complement(37224..37789)
                     /locus_tag="U2K71_000544"
                     /pseudo
     CDS             complement(37224..37789)
                     /locus_tag="U2K71_000544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294436.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(37968..38624)
                     /locus_tag="U2K71_000545"
     CDS             complement(37968..38624)
                     /locus_tag="U2K71_000545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314655.1"
                     /translation="MRFFTTFTIIMIAVLFIFLDIAKRNTAFLLYRVLLRAGLITFIS
                     IVGFFLFTVIVFIWRTPAPPLPEITYGEFPFRLEYELNEELHVIEDTLIVEFDGFGMN
                     EGIGRYRRWTSRLASGEDLVLLLEVSDNKQIFYFPGPANYYMGDRLNGYNHTFPSASF
                     IERERGITRRDILHDKELLEQFGPLDQNTINEEELLNQYNIRLVNWEISEPIVNNFGD
                     "
     gene            complement(39192..40127)
                     /gene="whiA"
                     /locus_tag="U2K71_000546"
     CDS             complement(39192..40127)
                     /gene="whiA"
                     /locus_tag="U2K71_000546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein WhiA"
                     /protein_id="HEM7314656.1"
                     /translation="MSFAADVKKELTGLEVHREHAKAELAALLRMNGSLGIVNQQFIL
                     NVQTENAAIARRIYSLLKDHYQVRSELLVRKKMKLKKNNVYIVRLKQGTKDVLMDLDI
                     MDGMMFNGHVSDEIMGNSQKMRSYLRGAFMASGSVNNPETSRYHLEIFSIYEQHNQDI
                     CDMLNYYGLHARTLERRNGYISYLKGAEKIADFLTLIGATNSMLRFEDVRIVRDMRNS
                     VNRLVNCETANLNKTIDAASKQIENIELIEAKVGLHALPEKLQEIAELRLQHPEVSLK
                     ELGEMIPSGAISKSGINHRIRKINEFAEKLREEAV"
     gene            complement(40161..41159)
                     /locus_tag="U2K71_000547"
     CDS             complement(40161..41159)
                     /locus_tag="U2K71_000547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YvcK family protein"
                     /protein_id="HEM7314657.1"
                     /translation="MKMKTYRIRKPKIVVVGGGTGLPVILKSLRNQGADITAVVTVAD
                     DGGSSGAIRESIAMAPPGDLRNVLVALSDMPQFYEDIFQYRFKKEDQFLANHTIGNLI
                     IAAVSEMRGSTYEAIQLLAKMMHVDGHVYPSSETPLTLHAVFKDGTTAVGESKIAIDR
                     KTIDRVYVCNTNDKSEAKAARKVVSAIMDADMVVLGPGSLFTSILPNLVIPEIGEAML
                     KTTAETVYICNIMTQKGETEHFTDADHIRVLHKHLNQPFIDTVLVNTEKVPEDYMDPE
                     IYDEYLVQVAHDFNGLRNEGCRVISTDFLELKDGGVFHDGDKVVEELFRLVFGSKY"
     gene            complement(41156..42040)
                     /gene="rapZ"
                     /locus_tag="U2K71_000548"
     CDS             complement(41156..42040)
                     /gene="rapZ"
                     /locus_tag="U2K71_000548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNase adapter RapZ"
                     /protein_id="HEM7314658.1"
                     /translation="MTDNLQLVIITGMSGAGKTVAIQSFEDMGYFCIDNMPPRLIPKF
                     WELIKESGKVTKMALGVDLRSRSFFEEIQNMLIEIENTNFIDTSILFLDASDEELVAR
                     YKETRRAHPMAMDGLITEGIRKERAILDDLKAQASVIIDTTTLTPRQLREKINESFKS
                     KGDQGFRIEFVSFGFKYGLPIDSDIVMDVRFLPNPHYIPELRPLTGKDQPVYDYVMSF
                     DETQSFYHKFLDLLETVLPGYIKEGKSSLTVAIGCTGGQHRSVALTERLANALGKNYK
                     VNVTHRDKDKRKETVNRS"
     gene            complement(42175..42906)
                     /locus_tag="U2K71_000549"
     CDS             complement(42175..42906)
                     /locus_tag="U2K71_000549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate racemase"
                     /protein_id="HEM7314659.1"
                     /translation="MENFFSILGGMGTMATESFVRLINHRTKATKDQEYLNYVLFNHA
                     TVPDRTAYILDRSEENPMPFLLDDIEKQNLLQPNFIVLTCNTAHYFFEELQAATDIPI
                     LHMPREAANELVRQHTTGRVAILGTEGSMKAGIYEREVKNLGFETVIPDTVLQEKINY
                     LIYHEIKESDYLNQELYYEILEEAVERLNCEKVILGCTELSLMHEFAEDNHYPVIDAQ
                     SILADRTIERALAERSKALDTASEK"
     gene            complement(42910..44175)
                     /locus_tag="U2K71_000550"
     CDS             complement(42910..44175)
                     /locus_tag="U2K71_000550"
                     /EC_number="6.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-aspartate ligase"
                     /protein_id="HEM7314660.1"
                     /translation="MMNSIENEEFIPILLGSDMNVYGMARSFNEAYGKICQAYASDQL
                     APTRYSKIVNVEVIPGFDKDPVFIETMLRLAKERYSDKSKKYLLIACGDGYAELISQH
                     KQELSEYFICPYIDYSLFERLINKVSFYEVCEEYDLPYPKTLIVREEMLVNGHLEQEL
                     PFEFPVALKPANSVEYLSVQFEGRKKAFILETREEFDLILGRIYEAGYKSEMIVQDFI
                     PGDDSNMRVLNAYVDEDHQVRMMCLGHPLLEDPTPASIGNYVVIMPDYNEKIYQTIKA
                     FLEKIEYTGFANFDMKYDPRDGEYKLFEINLRQGRSSFFVTLNGLNLARFVTEDRVFN
                     KPFVETTYGTNQSDKARLWMGVPKKIFLEYARENEDKKLAEQMIKENRYGTTVFYEKD
                     RSIKRWLLMKYMFHNYIPRFKKYFHVKEG"
     gene            complement(44438..47254)
                     /gene="uvrA"
                     /locus_tag="U2K71_000551"
     CDS             complement(44438..47254)
                     /gene="uvrA"
                     /locus_tag="U2K71_000551"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HEM7314661.1"
                     /translation="MANDKITIHGARAHNLKNIDVTIPRDKFVVVTGLSGSGKSSLAF
                     DTLYAEGQRRYVESLSAYARQFLGQMDKPDVDSIDGLSPAISIDQKTTSKNPRSTVGT
                     VTEINDYLRLLFARVGHPICPNDHIEITSQSVDQMADQVMALPERTKIQVLAPIVVKK
                     KGQHKKIFERIQKEGYVRVRVDGEIYDLSEVPELEKNKKHDIAIVIDRIIVKEGIRSR
                     LFDSLEAALRLADGYALVDVIGEEEMLFSEHYACPYCGFTVGELEPRLFSFNAPFGAC
                     PDCDGLGVKLEVDQDLVIPDRTKTLSQGAIVPWNPISSQYYPQMLAQACESFGIDLDT
                     PFEELPEEHQDIILNGTDDLFHFHYENDFGGVRDVETTFEGVLKNIERRYHDTNSDFT
                     REQMRLYMTELTCQTCKGYRLNPQALSVQINGTNIGQTNELSIQKAVDFFSNLTLSEQ
                     EKVIAKPILKEVNDRLSFLENVGLDYLTLSRASGTLSGGEAQRIRLATQIGSNLSGVL
                     YILDEPSIGLHQRDNDRLIGSLKKMRDLGNTLVVVEHDEDTMWAADYLIDVGPGAGEQ
                     GGQIVAAGTPEEVANDENSLTGKYLSGKKSIPVPKERRKGNGKAIKITGASENNLKNI
                     SVEFPLGEFVAVTGVSGSGKSTLVNSILKKSLAQKLNKNSAKPGKFKTISGYESIEKI
                     IDIDQSPIGRTPRSNPATYTSVFDDIRGLFAQTNEAKMRGYKKGRFSFNVKGGRCEAC
                     RGDGIIKIEMHFLPDVYVPCEVCHGKRYNSETLEVHYKGKSIADILEMTVEDAVEFFK
                     HIPKIHRKLQTIVDVGLGYVTMGQPATTLSGGEAQRMKLASELHKISNGKNFYILDEP
                     TTGLHSDDIARLLHVLQRLVDAGNTVLVIEHNLDVIKTADYIIDLGPEGGEGGGTILA
                     TGTPEEIINVKESYTGHYLKKIMV"
     gene            complement(47264..49258)
                     /gene="uvrB"
                     /locus_tag="U2K71_000552"
     CDS             complement(47264..49258)
                     /gene="uvrB"
                     /locus_tag="U2K71_000552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HEM7314662.1"
                     /translation="MIERDTSRHFELVSKYQPAGDQPEAINQLVDGVVGGKKAQILLG
                     ATGTGKTYTISNLIEKVNKPTLIIAHNKTLAGQLYGEFKEFFPNNAVEYFVSYYDYYQ
                     PEAYVPSSDTYIEKDSSVNDEIDKLRHSATSSLLERNDVIVIASVSCIFGLGSPFEYQ
                     KQVVSIRQGAELDRNQLIRDLVSIQFERNDIDFQRGRFRVRGDVVEIFPASRDERALR
                     VEFFGDEVERIREVNALTGEVLGETEHVAIFPATHFVTNDEHMEHAVANIKAELEQRL
                     TVLRNENKLLEAQRLEQRTNYDIEMMLEMGYTSGIENYSRHMDGRKEGEPPYTLLDFF
                     PEDFLIVADESHVTMPQVRGMYNGDRARKQMLVDYGFRLPSALDNRPLRLEEFEKHVN
                     QIIYVSATPGPYEHEQTDTVIQQIIRPTGLLDPVIEVRPIMGQIDDLVGEINERVEKD
                     QRVFVTTLTKKMAEDLTDYFKELGIKVKYLHSDIKTLERTEIIRDLRLGEFDVLVGIN
                     LLREGLDVPEVSLVAILDADKEGFLRSERSLVQTIGRAARNEEGKVIMYADKVTDSMR
                     LAMDETSRRRTIQQKYNEEHGIVPKTIIKEIRDLISITKESEDDTKEAVQVSYEEMTK
                     EEKDTLLMKLEKEMKNAAKALDFETAANVRDMILELKASK"
     gene            49517..51019
                     /locus_tag="U2K71_000553"
     CDS             49517..51019
                     /locus_tag="U2K71_000553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein/permease"
                     /protein_id="HEM7314663.1"
                     /translation="MKRKHLFHSFVFLMTGLVTLAFGHVAQADNTTTAESSTSETMQK
                     IEPKKDVYVIASDSAFAPFEFQNAKGEYEGIDVKLMENIAKLQGFNIRMDYRGFSAAL
                     QALESGQADGMIAGMTVTDERKKSYDFSDSYFDSGIQLAVKKGDTSIKSYEDLKGKTV
                     GAKVGTESAEFLSDHESEYGYSIKYFDAADQLYDGLKVGAIDAMMDDYPVIGYAIKQG
                     QELETPIKKESGGKYAFAVKKGQNPELLNMFNEGLKELKRTGEYDKIIKEYVADGSET
                     SSESSTADESTFLGLIQNNYQALLKGLWRTILLTLISFALAIVIGVIFGLFSVAPIRA
                     LRTIASIYVDIIRGIPMMVLAFFIFFGLPGIVGFTIPDFLAGVITLTLNASAYIAEIV
                     RGGINAVPVGQMEASRSLGLSYNRTMQKIILPQAIRIMIPSFVNQFVISLKDTTIISA
                     IGVVELLQTGKIIVARTTQSTYVYLIIAIMYLILITALTKLAKVLEKKVK"
     gene            51021..51758
                     /locus_tag="U2K71_000554"
     CDS             51021..51758
                     /locus_tag="U2K71_000554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010760672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM7314664.1"
                     /translation="MAEKILVEHLVKKYGDNTVLNDINVSINEGDVVCIIGPSGSGKS
                     TFLRCLNRLEEPSDGEIIIDGAHLMDKSTDINLVRQHIGMVFQHFNLFPHLSILENII
                     LAPMDLKKESREEAEKKAIKLLQTVGLEDKKDMYPEMLSGGQKQRVAIARALAMNPDI
                     MLFDEPTSALDPEMVGDVLNVMKKLAEQGMTMVIVTHEMGFAKEVANRVMFIDDGNFL
                     EDGTPQQIFENPQNERTKDFLNKVLNI"
     gene            complement(52126..53292)
                     /gene="dnaJ"
                     /locus_tag="U2K71_000555"
     CDS             complement(52126..53292)
                     /gene="dnaJ"
                     /locus_tag="U2K71_000555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HEM7314665.1"
                     /translation="MATKRDYYEVLGLSKGASEDEIKKAYRKLSKKYHPDINKEPDAE
                     EKFKEVSEAYEILSDPQKRAAYDQYGHAGTDPNYGAGGAGGGFGGFGGFSGGGFGGFE
                     DIFDSFFGGGGRSVDPNAPRQGADLQYTVDLSFEEAIFGVEKEIKYNREEICHTCGGN
                     GAKPGTQPTTCHKCHGAGTINVERQTPLGRVMSRQTCDVCHGTGKEIKEPCPTCHGSG
                     HEKKAHSVKVNVPAGVEEGQQMRLAGQGEAGENGGPFGDLYVVFRVEESDIFDREGSE
                     IYYELPLSFVQAALGDEVQVPTVHGDVKLKIPAGTQTGTNFRLRGKGAPKLRGGATGD
                     QHVKVKLITPKNLNEQQREALRAFAEAGGIKVEEQQEDGFFDKFKDAFGGKKRK"
     gene            complement(53443..55272)
                     /gene="dnaK"
                     /locus_tag="U2K71_000556"
     CDS             complement(53443..55272)
                     /gene="dnaK"
                     /locus_tag="U2K71_000556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016635026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HEM7314666.1"
                     /translation="MSKIIGIDLGTTNSAVAVLEGNEAKIIANPEGNRTTPSVVSFKN
                     GEIQVGEVAKRQAVTNPNTISSIKRHIGEAGYKVDVEGKSYTPQEISAMILQYIKGFA
                     EDYLGEKVEKAVITVPAYFNDAQRQATKDAGKIAGLEVERIVNEPTAAALAYGLDKTD
                     RDEKILVFDLGGGTFDVSILELGDGVFDVLSTAGDNHLGGDDFDNKIIDYMVAEFKKE
                     NSIDLSQDKMALQRLKDAAEKAKKDLSGVSSTQISLPFITAGESGPLHLEMTLTRAKF
                     DELTADLVERTKVPVRQALKDAGLSQSEIDEVILVGGSTRIPAVVEAVRKETGKEPNK
                     SVNPDEVVAMGAAIQGGVITGDVKDVVLLDVTPLSLGIETMGGVFTKLIDRNTTIPTS
                     KSQVFSTAADNQPAVDIHVLQGERPMAADNKTLGRFQLTDIPAAPRGIPQIEVTFDID
                     KNGIVNVSAKDLGTQKEQKITIKSSSGLTDEEIERMVKDAEANAEADKARKEEVDLRN
                     DVDALLFSVDKTLKELEGKVDEEEVKKAETARDELKAAVEANNIEEMKTKRDALNEIV
                     QNLTVKLYEQAAQQQAQENPEAAQGGADDVVDADFEEVDGDDK"
     gene            complement(55323..55886)
                     /gene="grpE"
                     /locus_tag="U2K71_000557"
     CDS             complement(55323..55886)
                     /gene="grpE"
                     /locus_tag="U2K71_000557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HEM7314667.1"
                     /translation="MKENQEELDKEMTDAQPEPEIDVEAAEDSGISEAEAEEFETAKL
                     KAELEEMEDKYLRARAEIANMANRGKNEREQLQKYRSQDLAKKLLPSIDNLERALATE
                     VSDDQGAGLKKGVEMVLESLRNALEEEGIEKIPAKGEAFDPNLHQAVQTVPATEDTPA
                     DTIVEVLQEGYKLHDRVLRPTMVIVAQ"
     gene            complement(55980..57023)
                     /gene="hrcA"
                     /locus_tag="U2K71_000558"
     CDS             complement(55980..57023)
                     /gene="hrcA"
                     /locus_tag="U2K71_000558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HEM7314668.1"
                     /translation="MLTKRQNDILRLIIQHYTSSGVPVGSKTLMSEGVKASSATIRND
                     MKALEDEGLLLKTHSSSGRIPSALGYRYYVDHLLKPARVAEDDLLLIRQSLGREFHEI
                     NEIIKQSAEILSELTSYTTFSLGPEIKDRRLTGFRIVPLNNRQIIAIVVTDMGNVESQ
                     VFTLPEYMGSQELEKMVQIVNDRLVGDPLMTVYHKLRTEIPMILHKYFQTTEGMSQLF
                     DTVLGHVFEDKVYVSGQMNLLDYEPRQDLDQFKSMFSFMKDSDGLTQMITPMDSNIHI
                     KIGAELGNDLLQNMSMIQASYEIDGHGRGTIALLGPTSMPYSRMFGLVDVYRRELAAK
                     LADYYRSLDLSNS"
     gene            complement(57257..58423)
                     /locus_tag="U2K71_000559"
     CDS             complement(57257..58423)
                     /locus_tag="U2K71_000559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxygen-independent coproporphyrinogen III
                     oxidase"
                     /protein_id="HEM7314669.1"
                     /translation="MIETINPAENKVSAYIHIPFCEHICYYCDFNKVFLEGQPVDEYV
                     EMLLNEMKIMLESHPTANLETLYVGGGTPTSLSAKQLDRLLSGAREILPFQNGNEFTV
                     EANPGDLTREKLQVMKNYGVNRLSMGVQTFDNRLLKKIGRKHTAEDVYQTMQFLEAEN
                     FTNVSIDLIYALPGQTLEGYLDTLEKALALDLPHYSLYSLILENKTMFMNWVRQGRLE
                     LPDQETETRMFEETIEAMEKHGRHQYEISNFGLSGHESQHNLMYWNNAHYFGFGAGAS
                     GYLGDIRYRNKGPIQHYLRPLRANERPVLEEEKLTRKNQIEEEMFLGLRKKTGITKKH
                     FYDRYQKTIESVYGDVLRELEQEGLLVNEEDRVYLTPRGTFLGNNVFERFLFEK"
     gene            complement(58439..58666)
                     /locus_tag="U2K71_000560"
     CDS             complement(58439..58666)
                     /locus_tag="U2K71_000560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine deaminase"
                     /protein_id="HEM7314670.1"
                     /translation="MKIAFHIFADFDLMPKVSFAITVYREAVKAPCSSDDNKQAFLEL
                     GFLDSKYAAYFNELVLERDFKKVENRHDFST"
     gene            59150..59740
                     /locus_tag="U2K71_000561"
     CDS             59150..59740
                     /locus_tag="U2K71_000561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HEM7314671.1"
                     /translation="MKTAITFLLEEYADWEGAYITSTLNQNEKWQNKTASIQSEVTSI
                     GGLKTKIDYLIEQIPEKVDLFIMIGGNSWDIKNHHLYQIIDKLLKTDVPVAAICGAVD
                     YLAQSGLLQGYKHTGNAQFPWLESENYQNKEDFIEQQAVKDRNLITANGTAPIEFTDL
                     ILQAVHFETEEIREKTIRLYKLGFYDYYKKYGDPFS"
     gene            59930..60643
                     /locus_tag="U2K71_000562"
     CDS             59930..60643
                     /locus_tag="U2K71_000562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314672.1"
                     /translation="MKKLGFILLLVSLFLVGCTSPSKENSDPSATTDKSSIRQTSSAE
                     KSSTQKSASTETNTSSTSSKVSEPNTSSTSSSTVETNISSSTIDTTSSQSSQSIDPLA
                     GYSDLQIEYARVWLAIRGNTFRDTSGDFELHVIHYPAGTPIAPYDAGSAVYPKDVVML
                     TGKYSYQGVLVYSSNHDGTITSYPVPSHWQMPADQTSYPAFIRETTQEIIDNASVVAI
                     PTGNPNDVKQLIDVTVIDN"
     gene            complement(60758..61768)
                     /locus_tag="U2K71_000563"
     CDS             complement(60758..61768)
                     /locus_tag="U2K71_000563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LD-carboxypeptidase"
                     /protein_id="HEM7314673.1"
                     /translation="MISYPLLERNATIGVTAPSSGVSEEHHSLLESALKRMEEKSFQV
                     VTGKTLWTQDKEKSGPARKRADEFNQMMQDENIQMIFPTWGGELLIEILDFVDFDALQ
                     PKWIVGYSDISTLLLAVTLKTGIATAHGTNLIDLRGEYLDETTAMWESVLKTREGESI
                     LQQSSEKYQKVWPHEHSSSSVFHLTEPTNWQSVSGDAVRMKGRLLGGCIDTIRHLIGT
                     PYGDVQSFKRKYNDGKPILWFLENCELSSVDLRRSLVQMKLAGWFDGSSGIMFGRSAA
                     EDLLDYKMKDVYEDLSKELKLPILYDIDCGHVPPQLTLINGAYAEVAVEDRKATVLQT
                     FI"
     gene            complement(61805..62287)
                     /locus_tag="U2K71_000564"
     CDS             complement(61805..62287)
                     /locus_tag="U2K71_000564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA editing protein"
                     /protein_id="HEM7314674.1"
                     /translation="MIDLAKKFLHENIYFELYHHRAIYTNEDALVIKKEQGFTGTETK
                     SLFLKDKKDNNYVYLTFTTKQTDFKALGKIVGKRLSIVSSERMEEQTGQKQGAVSPFG
                     YEKQIPIIIDEELLSNEKLVFAPGRPDQTMVIQTVDLNKIIQLLEMPIFLMPANEKNN
                     "
     gene            62437..62805
                     /locus_tag="U2K71_000565"
     CDS             62437..62805
                     /locus_tag="U2K71_000565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1801 domain-containing protein"
                     /protein_id="HEM7314675.1"
                     /translation="MEKFQPIINQIKEKEHQQKIKDLFAKITETYPQLDTTVKWNQPM
                     FTDHGTFIIAFSFAKNHFSIAPEKAAIRALEKNIQEAGYVYTDNVIKVPWKSVINWEL
                     IEQLISFNIEDKKGHDKFWR"
     gene            63270..63722
                     /locus_tag="U2K71_000566"
     CDS             63270..63722
                     /locus_tag="U2K71_000566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7314676.1"
                     /translation="MKDFYSVNELAEQLGVTTRSIRNYLHEGKLKGTKVGGQWKFSER
                     NLFEFLYGDQAEEAAKEMQRFMLNAPITMRFNLQYRDFTAINQFREQLVQYHNDVYAN
                     KKDRLLQYDLYKDNHAEILIGGNFNYVVNFSQWINEKLLIQTDISLVS"
     gene            complement(63846..64697)
                     /locus_tag="U2K71_000567"
     CDS             complement(63846..64697)
                     /locus_tag="U2K71_000567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transporter"
                     /protein_id="HEM7314677.1"
                     /translation="MGLLIALIPAVGWGIQPLILKKIGGRPTNEILGTGIGAVLVGLL
                     VQLFASPAGISVTTFLISLLSGAFWVIGQTGQYTSFNLIGVSKTMPISTALQLVGTSL
                     IGVFAFGEWSGSMGKIIGAAAIILLVFGSALTAISDDPGKKGGITKGISLLASTSIGY
                     WVYSALPKLVDASGLSIFFPQMIGVFLGAVIYVIFRKPTAFSEDKSWKATIVGAIFSV
                     SALAYIFSANENGVATAYIITQLNVVISTLGGMYILHENKTPKEKKLTIIGLLLIVVG
                     SMVTVFI"
     gene            complement(64710..65495)
                     /locus_tag="U2K71_000568"
     CDS             complement(64710..65495)
                     /locus_tag="U2K71_000568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM7314678.1"
                     /translation="MYKELENKVAVVTGGSKGIGTAIAQRFGKEKMNVVVNYNSDKEG
                     AEKAVKAIEEAGGKAVSVQADVSSEEGIQRLLDTALENFGTLDVWVNNAGMENQHPTH
                     ELSLEDWERVLKVNLTGVFIGTKAALNYFTENNKKGNIINMSSVHEQIPWPTFAHYAA
                     SKGGVKLFTETVAMEYAPKGIRINSIGPGAIRTPINAAKFDDPEQKELLESMIPLGRV
                     GEPKEIAATAAWLASDESSYVTGITLFADGGMTLYPSFQGGKG"
     gene            complement(65847..66788)
                     /gene="ribF"
                     /locus_tag="U2K71_000569"
     CDS             complement(65847..66788)
                     /gene="ribF"
                     /locus_tag="U2K71_000569"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="riboflavin biosynthesis protein RibF"
                     /protein_id="HEM7314679.1"
                     /translation="MNIIKIRHPYQSEQIPEDDVVLVLGFFDGVHIGHQKVIKTGMEI
                     AQKEGLKLALMTFNQHPSIVFKKIDPSSVKYLTSLKQKEEKMAALGIDYLYEVDFTSS
                     FAHLAPQEFVDQYIVGLHAKYAVSGFDYTYGPKDIADVAHFPEYAKGRFKIVTVSKEE
                     EQGEKISSTRIRGLLDSGQVKEAAKLLGCTYEVEGVVVHGDARGRLLGFPTANVKVKS
                     TVHLPKEGVYVSEIKIGETWYPAMGSIGHNDTFGEGRELTVELYILDFNQDIYGEHVA
                     VRWNQFLRDQVKFSSVEELIEQLKEDEQATAEYFSQQ"
     gene            complement(66792..67715)
                     /gene="truB"
                     /locus_tag="U2K71_000570"
     CDS             complement(66792..67715)
                     /gene="truB"
                     /locus_tag="U2K71_000570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HEM7314680.1"
                     /translation="MDGLLPLWKERGMTSHDCVFKLRKILHTKKIGHGGTLDPDVDGV
                     LPICIGKATKVIEYLTDSGKIYVGEITLGFSTTTEDRSGERVETKPVDMSLSEKKIDE
                     VMASFIGEITQIPPMYSAVKVNGKRLYEYARNNETVERPVRKAQIYRFIRTSELTRDE
                     VTGTISWRFEVECGKGTYVRTLAVDTGKKLGYPAHMSDLTRTASAGMSQKDALTLEEV
                     AQAVKNGTIDDCLLPIEKGVERFKRVDITDEVWQKVKNGMRLDYTVFALPEMPSEEIA
                     LFYKGKVVSIYQPNPKEKNKLKPSKVLRNEE"
     gene            complement(68083..70445)
                     /locus_tag="U2K71_000571"
                     /pseudo
     CDS             complement(68083..70445)
                     /locus_tag="U2K71_000571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625383.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoketolase family protein"
     gene            70893..71066
                     /locus_tag="U2K71_000572"
     CDS             70893..71066
                     /locus_tag="U2K71_000572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7314681.1"
                     /translation="MIFLLTVNGPTNSNFYGAGSTDLLVTWLYLTVTKIDYNLASVIG
                     ILIFIYMQKFIYY"
BASE COUNT        19783 a        15314 c        12375 g        23722 t
ORIGIN      
        1 ttcttcatac tcaaaacgaa acggcttttt cacaacctct tacttatact tattcacaaa
       61 atccaatgtc tgctggtaac aattttgcga ttcttttagc gtgtaatcaa attggtattc
      121 atgggtaatc tctttttttg tatctgtata aaacaaggat tgcacgggta cctttaattc
      181 tgtcaaccga ttttctaatg cttgaccttg ctcctggaat gagtatgcat ttccgtcagt
      241 tatatacgtt ggcggaaatt ccttagaaac atggtcagcc acactagctt ctttcaattg
      301 ctcactcgtt ttccaatctt ttgttccaat caacgaccac gcaacggttt tgacgaaaaa
      361 cttcataaat cgatcatccg aatgctgctg tgctgttagc ttcaaatcaa caggtccgca
      421 ataagaaatg gttcctttca gactatcttt tggaagtagc tgtttgattt tcatttgttt
      481 agcatacgca tcattcgttt gtatagctgc atattgcaat gcgatttgtg aacctgcact
      541 atctccacca aaaaataatt gttccatgtc tagcaatttg tcgtcttttt cttcagctaa
      601 taaatctttg atcagttcat tcacttgaag aacttgatta gggtattctg aatcaggcgc
      661 taattcataa ttcatcgcaa ccactgcgat gttggcgtct gaaactaatt ttgtcgcaaa
      721 ttctttgaca cccgatttat ctccgccgac gaatcctcca ccatgtaccc aaaatagaac
      781 aggaacaggc cccttgctgt tttttggata gtagatgtca taagtattct ctttatactt
      841 ggactcatac gtcaaatctg tttttttcgc cacgtttttt tctgcttgat cgaacgtttt
      901 tttatcagtg atttgaacag tatttgaaaa catatggttg atcaagaatg ctccaggtct
      961 gggggaaagc tggaaggcaa gaaagattgc aaacgcgatc ccaagaatga ttccgaagaa
     1021 ccatccgata aatttaagaa ttttcttttt cataaggctc cctcagtagt tagtattaag
     1081 cattctgatt ataccatagt gttatttcct atcaagctct cctagatgat ttaatgattt
     1141 tccccaaact tcttctgcaa tgcttttgac aaaagaaatt ttttccattg ttcttcaaac
     1201 aaaacatttt cttcttctgt tgaagcaaat ccacattgag gacttaagca caaacgatcc
     1261 aaaggcagat atcgtgtcgc ttcgtagatc cggtcaatca cttgctggtg ctcttccatt
     1321 tcgccatttt tcgttacagt caaaatctaa gacaactaat ttatcttcag agactttctc
     1381 taaaagaaca aaactcccag cacgttcact atcgtattct aaataatagg cacggacatt
     1441 ttcttgagta aaataatggg tctgctacgt cgtcatcccc cctgatgccg ccaaaatcga
     1501 ataataattt ccgcgtcaaa catgtatatt tagtgttgaa tcatttgaga atatgaaaat
     1561 aaaaatcttt ttgatcaatc agttaatcaa attgaaataa cgaaaaatct gagacagaat
     1621 gacgttcatt ctgacccaga tttgattttg tacaacccat cagtccttat tgacaaagag
     1681 ccactaaaaa gactaagaaa caataacaaa agttaacact tcttagtctt tctttagcac
     1741 gtctatttat taaagatttt agggcctttt ctcatgtttt tacgatcatt cttaggtgtt
     1801 tccatttgtt tggatcggcc tccaccttgt ttctttttga tcaattcttt cattttttct
     1861 ttttggtcca tttttattct cctttaacga atgaagcgac taattcgata tgatgggttt
     1921 gcgggaaaag atctacaggc tgaactttct tcacggcata acctgcttct gcaaataact
     1981 tagcatctct tgcaaaagtt gccggattac atgagatata gacgattcgt tctggtgaca
     2041 tttcgattgc tgattcaata aaatgatgat ctaatccttt tcgtggaggg tcaactacga
     2101 tcacagatgg tttgatccct ttttcttgcc attttttcat aactttttct gcttttccga
     2161 cttcataatg aacattttcg attttattat ttaaagcatt gaactgtgca tcttcaattg
     2221 cttctggaat aacttccatc ccatacacct tatccactct gtctgctaaa gacaagccaa
     2281 tcgtacctat tcctgaataa gcgtcgatca caatgtcttt tttatctaac tcggcaaact
     2341 cgatggctgt ttggtacaga tactctgctt gttgagtatt tacttgataa aatgactttg
     2401 ctgagattcg ataggttttt cctaatagct gatcttcgat atagtctctg ccatataaaa
     2461 ctttttcttt gtctcctaaa atcacatttg ttttttcttc attgatattt tggatgaccg
     2521 aaaccacttc tggcaattct tcatgaatag cttcagcaat ttccttggat ttaaagaatt
     2581 tttctttacg agtaactaaa acgaccatca tttcatggga ataatgacca cgacggataa
     2641 tgatgtgacg aagctgtcct tcattttttt cttcattata tcctcgtact tggaaccgtt
     2701 gcaagatatc tctgactact ttgattgctt gatcgatgac aggatcttgg atataaaaat
     2761 cttcgatagg gactagatca tggctattct ttttgtaaaa accggtggct aactgtccat
     2821 cgatacggcg aacagggatc tgtgctttat tacggtagcc gaatggttct gccattccga
     2881 ccgttggaag tacaggaact tctggcatct tagcaatctt cgccattaca tgctctactt
     2941 gtttttgttt gaagacaagc tgttgttcat acttcaaatg ggctaaaggt gcgataccta
     3001 tgcgaagtaa tgcattgttt tctacaggct gacgatcagg tgattgtgta ataaatgttt
     3061 cgacttttcc atatccaaac ttattgcctg cttttactac tagtatttct acttcttctc
     3121 cagggagtgc attttcgata aataatggat aaccatcgat ttttgctacc cccattcctt
     3181 cataactcaa gtcgacgatt tcgactgtat atcgttcgtt tttctttact atttgctgtt
     3241 ctgtcatatt ttgctccttt aagcctattt ttcaatccta ctgaactcta ctataaatca
     3301 gctaattaca caactaaaac cggcactgac cttactacgt tcagcaccgg cttttgatta
     3361 attcttttga ttaggtgttt cgtctgctat ttttccgtca ccgtcgatat cttcatccgt
     3421 taaacgttcg acttctttta cgaattcttt gctgacaacc tctaattctt cttcatctgt
     3481 acctgtgatt gcttcatcag gaatctgatc ggtattggca aagaactcaa tgtgttgatg
     3541 aagattttgg aaatgcatcg gtgcttcccc gccgtattca ccatctaagt tgatcattac
     3601 tggtcgattg atatcatctt ctgtttcaac gaacaaactg cttgttttgg tatagattat
     3661 tcttggatca tcgacatgtc gaccgccatt cagcatgagt gctgcaatat gcaagatctc
     3721 aaagatattt gctgtcttca caatgattaa agaaaacttc ccatcatcca atttggcatc
     3781 tggcgcgatc tgttcgaatc ctccaacaga gttcgtcagc cctaggaaaa acatcgaagc
     3841 atttccgata tattcccctt catcatactt caagcgcatt ttgattggct ttactcgagg
     3901 gagcatctct gctcctttcg caagataagc aagataacca aaaatactct ttagttctga
     3961 tggaacttca taagtcaatt ctgtcaaata gccacctgca gcgatattga tgaagtaact
     4021 gttatcagaa aggccgatgt ccatttttac ggtttggtgt ttttttatca cttctgctgc
     4081 tgctttaata ttatctctag gaatttttaa tgctcgagca tagtcgttcg ttgtaccagc
     4141 tgggatgatg gccattttag gacggtgttc aagcggagcg attccattaa ccacttcgtt
     4201 gattgtgcca tcgccaccag ctgccactac tagatcaaaa cctagttctg cgactctaca
     4261 tgcttcattc ttggctgaat ctggttctgg tgtagttgca taggcgctgg cttcaaatcc
     4321 tgcttcttct aatgcctgta ggatatccgc caaatttttt ttcaataatt cttttcccga
     4381 ggtggggtta taaattacgc gtgctttttt cattttcagc ctctatcgtt tcgctaattc
     4441 atctttaagt agcttattca cgacaccagg atttgcttgt cctttagttg ctttcatgat
     4501 ttgaccgact aggaagccaa ccgcacgatc ttttccattt ttgaaatcat cgacagattg
     4561 ttggttgtta tccaaaattt cattaatgat tggcagtagt ttgttcggat cagataattg
     4621 cacccaacca tttttttcaa ctacttcttg ggcttctccg ccatttgtga tcaattcacg
     4681 gaagactttt ttggcgattt ttgagctgat cgttccatcg gcgatcaaac gaatcattcc
     4741 tgctaagttc tcaggtgtca attttgtctc agctaattcc agtttctcac tgttcaagta
     4801 agcagaaact tcacccatca accagttaga tgcctgcttg gcatctgcac cattttccaa
     4861 aacagcttca aaaaaatcag acatttcttt tgtaagtgtc aataccattg cgtcatactc
     4921 tggaagtcca aactcacgga tatagcgttc gcgacgagaa gctggcatct caggcaaact
     4981 agcttttact ttttgaatcc attcgtcatc gatcacgaat ttcgggatat caggctctgg
     5041 gaagtaacga taatcactag acccttcttt gacacgcatc aaaactgttt tattcgttgt
     5101 ttcgtcaaaa cggcgtgttt cttgttggat ttctcctcct gaaagaagaa cttttgcttg
     5161 acgtttttct tcaaaagcta aacctttttt cacgaaagat aaagagttca agtttttcaa
     5221 ttctgctttc gtaccgaact cttcttgtcc ataagggcga agagaaatat tggcatcaca
     5281 gcgcatagag ccttcttcca ttttcacatc gctgacttct gtaaattgga taattgatcg
     5341 caatgcttct agataagcgt atgcttcttc tggtgaacgc atatcagctt cagaaacgat
     5401 ttcgatcaaa ggcgtgcctt gtcggttcaa atcaacgtaa gaataaccgc caataccatg
     5461 catgtttttt ccagcatctt cttctaaatg gacacgttcg atccggattt ttttcttttt
     5521 accttctact tcgatttcga tccatccgtc atgaccgatt ggttgatcaa attgggaaat
     5581 ttgatatgct tttggattat ctgggtaaaa gtaatttttc cggtcaaaat gagtatcttt
     5641 agagatttca cagtttaatg ccaaagctgc tttcatcccg aattccagcg cagctttatt
     5701 cattactggc agcacgcctg gataacccca gtcgatcacg tttgtattgc tgtttggttc
     5761 tgcaccaaaa tgagctggcg ctggagagaa aatttttgag ttggttttta actcgacgtg
     5821 gacttctagt ccaatgactg tttcaaaatt cattcgtctt tccctcctaa aatagctggt
     5881 tgttgtttat ggaattctgt tgcttgttca aacgcatatg ccgcttgata catcgttttt
     5941 tcatcaaaat aattaccgat gatctgcaat ccaactggta atccttctga taaacctgcc
     6001 ggtacagaca tacctggaag tccagccaag ttgacaggga tcgttaagat gtcactcatg
     6061 tacattgtga ttggatcgtt aatgttctca ccgattccga atgcaactgt tggtgaagat
     6121 ggcccgatga tcaagtcata atctgcaaag actttttcaa aatctcgttt gatcaatgta
     6181 cggacttgtc ccgctttttt gaagtaagcg tcataataac cagcacttaa ggagaatgta
     6241 cctaacatga tacggcgttt tacttcttca ccgaatcctt ctgaacgtga gttgacatat
     6301 acatcatcta atgatttcac attttctgag cgataaccgt agcggatacc atcgaatcgt
     6361 tgcaagttgg aacttgcttc tgatgaggca atgatataat aaacagccac accgtatttt
     6421 gaatgaggca agctgacttc ttccacaaca gcacctaatg aacggaatgt ttctgctgct
     6481 ttcaatacag cttcttttac gccaggggcc acaccttcgc ctaagtattc tttaggtaaa
     6541 gcaatcttca ttcctttgat gtcgcctgtc agtccctctg caaaatcagg aactgaaacg
     6601 cctgaagaag taccgtcttt ttcgtcataa ccgctgatag ctgtcaatgc taatgcatta
     6661 tctttcaccg tacgggtcat tggtccgatt tggtcaagag atgaagcaaa ggcaatcaaa
     6721 ccaaaacgtg agacacggcc ataagtaggt ttcatcccta cgatgccgtt aaaagctgct
     6781 ggttggcgga tacttccccc agtatcacta cctaaagaaa ctggaatttg tccagctgca
     6841 actgctgctg cagaaccacc agaagagcca ccaggtactt ttgttgggtc ccaagcattt
     6901 tttgttttct tgaaataaga agtttctgta cttccaccca tagcaaattc atccatgttt
     6961 agcttaccaa cagggatcat atctgcttga tagattttat ccatgaccgt tgcatcataa
     7021 attggttcaa aattgtaaag gatcttagat gcagcggttg tcaagatatc ttttgttacg
     7081 atattatcct tgataccaat tggaatacca gcaaggacgt tgctttcatc aatccctttt
     7141 tcatctaaag cttttgcctg ttccaatgct ttttcttcgt tcaatgtaat gaatgcttcg
     7201 acctcaggct ctgtttcttt gattcgttca aatgttgctt ttgtcaaatc ttgtacagtg
     7261 atttctttcg atacaagcat atgatgcagt tcttctagag aacgattgtg taattctgtc
     7321 attatgcgcc agcctcccca ttatcgatga ttgcgggtac tttgataaag ccgtcttctg
     7381 tttccggcac atttttcaat aattcatcgc ggttccatcc agcttgcgcc cgatcttgtc
     7441 gcatcacgtt tactgtttca accacattcg acgtaaatgg aacaccttct gtatctactt
     7501 cttccagcat ttcaaccata tcgatgattt tacctaattg gtcggtaaaa cttgcaagtt
     7561 cttcatctgc gaacgcaagt tttgctaatt tggcaacgtg tttcacttgt tcttcactaa
     7621 tcgccatact accccttctt tctaaacgag ttaaaaactt tgctctctat tttaacacaa
     7681 gagtgccgtg tctgaaattg tttattgaaa tttgtctgaa aaaactcatt tttcattctt
     7741 ctactgagcg acttttagcc aatgcatctg ccatttgatc ttctgtccaa acttcgattc
     7801 ctaattcttg tgctttggtc agcttgctgc cagcatcttc tcctgctacc acgatatccg
     7861 tttttttcga tacgctacca gtcactttcc cgccaagatt ttcgatcgtc tcttttgctt
     7921 cttcacgagt aaatcgtgtt agtttaccag taaggactac tgttttttct ttaaaaggag
     7981 actcgacttc ttgcaattgg gcatttcgaa gccctttgta ttcgaaattg acgcctgctt
     8041 gttttaattc attcatcaat tcatggactt cttcgttact gaaataagtg actacactgt
     8101 cggcaatgat atcaccgatc gtatcaagcg caataatcga ctcatagtca cttttactca
     8161 atgtttctag atcaccgaaa tgttctgcta aaatttttgc tgctttcgct ccgacatgtc
     8221 ggattcctaa cccgaaaatc aaccgctcga ccgaattatc tttgctgttg tcgattgctg
     8281 tcaaaatatt atttgctgat ttttctttta ttttgtccaa cgtcaataac tcttcttctg
     8341 tcaatttgta aagatcagca acatctgcta ctagtttttt atcatacatc tgttctagta
     8401 cgcgcggccc caacccatcg atattcatcg catttcttga aacaaagtgg ttcaaacctt
     8461 ctttcatttg agcagggcat ttaggattga tgcaccgtaa ggctacttct tcatccaaat
     8521 ggacaagttc acttccacaa actggacaat gtgtaggcaa ttggtattct tcgctgtctt
     8581 taggtcgttt atctaacacg acttgcgaaa cttctgggat gatgtctccc gctttataaa
     8641 tcagaactgt atctttcaag cggatatcct tcaacttaat ataatcaccg ttatgcaggc
     8701 tggcacggct tactgtcgtt cctgctactc ggactggtgt catgatagct gttggcgtta
     8761 ctacccctgt tcgaccgact gtccattcga tgttttcgat tagcgtttca acttcttctg
     8821 gcggaaactt gtaagctgct gcccatctcg gtgctttgac agtaaaacca agttgatctt
     8881 gtagcgagaa ttcattcact ttgatgacga ttccatcgat ttcatagggc aaatccacac
     8941 gtttgtcatg atattcttca atgtatgacc acacttcatc aatcgaatga catagacgtt
     9001 tttcccgatt cgtgtggaat ccaatttttt ctagctcttc tagcgcatca tactgggttt
     9061 ttgcttgcat aggaccgaaa tcagctactg tgtataaaaa agtgtccaga tttcttttcg
     9121 ctgtgatttt agaatctaac tgacgcaaag aaccagctgc ggcgttccga ggattcgcaa
     9181 agatatcttt tccctctgct tctctgtctt ggttcaattg gacaaatgac cgttttggca
     9241 tgaaacattc gcctcttact tcgatgttca ttggttcttt taatttgatt gggaccgacc
     9301 ggacagtttt taggttttca gtgatgtttt ctcctacagt tccatccccg cgagtcgcac
     9361 ctctcacgaa atttccatct tcataacgca aagaaacgga aagtccatct atttttagtt
     9421 cacagcaata gtcaactaca cgtcccactg ctttttgtac acgttgatca aaagctatca
     9481 gctcttcttt gctgaaaacg tcgttcaaac tgtaaagcgg aatatcatga gtgacttttt
     9541 caaaaccttc taatactttt ccgcctactc gttgagtggg agaatcagac gtgatcaaat
     9601 caggatattg ttcctcaatt gctactaatt ctgcgtattc tttatcataa acatagtctt
     9661 ccactgttgg tttatcttct acgtagtatt cgcgagacca ttggttcagt cttgtacgaa
     9721 gttcttctgc tcgtttagca gcttcgtcaa tcgtcatttc atgttccatc atttaccctc
     9781 cactactaag ctttttcaat tggcgcaaag gcagccaaaa gacgttttac tccttgactt
     9841 gggaaagcca catctagttc tagatccttc gacgtcccgc ttactcggac gactgttcct
     9901 agaccccatt ttttgtgttt gactttatct ccaggtttcc agctgttatt ttcccctcca
     9961 gtagcacttt tagagggaac aaccgctgtc tctggctgtt tatagctata tgctgttttt
    10021 ttagcgaata caggtgtttt tttaggcgta ggacgcatgc cttcgatttc cagtaactcc
    10081 tgatcgattt cttctacaaa acgagaaggt cggttatatt gcgtccgtcc atataatgtt
    10141 ctggagaaag catttgttaa atacaacgct tcttctgctc gcgtgatccc cacatatgcg
    10201 agacggcgtt cttcttccag ttcgctttct tccatcaatg ctcgagataa aggaaaaacc
    10261 ccctcttcta gaccaatcag gaatacaact gggaattcta atcctttggc tgcatgcaaa
    10321 gtcatcaatg tcacttggga agcatcttct tctaaattgt caatatcgga aacaagtgcc
    10381 agatcattta agaagaccgt tagtttttct tctggtgcat cagcgtcttc ttcattttgc
    10441 tgttcgaatt gtttgtcgaa ttcttgtgtg accgttaaaa attcctccaa gttttcgatg
    10501 cgtgcttgtg cttctaatgt attttggatt tttaagtctt ctagatatcc gctacgatcc
    10561 aaaacctctt tcgtcaattc tgtcactgtc aagtatggga tcatctgagt gacttcttgg
    10621 atcatctctc caaatgcacc taattgctgt cctgcttttc caccgatatt tgccagatcc
    10681 acattttgcg ctgcttcaag cagtggccat tcatgaagag aagcaaaact gcgtagtttt
    10741 tcgatagatc ctggtccgat gcctcgtttg ggcgaattaa cgatacgctc aaaactgatt
    10801 gaatcttgag gatttgccag cacattcaaa tacgctaaaa tatctttgat ttctttacga
    10861 tcgtagaact tgtgccctcc aaccatttta taaggaatat ttgctttcaa cagagtttct
    10921 tccattacac gagattgtgc gttcgtccgg tacaagatgg caaagtcgcc gtaattccga
    10981 tgattgcttc gaatctcttc ttgcatccga tctacgataa aacgggtttc gtctcgttca
    11041 ttatctgcac ggtagtaagt gatcttgttt ccttctttgt tttctgtcca caagtttttt
    11101 ggttttcgat tgctattgtt ttcaatgact tggtttgctg cgctaaggat gtttttcgtg
    11161 gagcgatagt tttgttctag caaaattact gcagcatctg gataatcttt ttcaaaatcc
    11221 aagatgttct gcatatctgc accgcgccag ccgtaaatac tttgatctgc atctcccacg
    11281 acgcacaggt tcctaaatct acctgccaat aaattgacta gcgtatactg tgcgtgattt
    11341 gtatcttggt actcatctac atgaatataa tggaatttgt tttgataata tgtgagtgaa
    11401 tcaggatgtt cttcaaataa tcgaatcgta ttcatgatca ggtcgtcaaa gtccatacat
    11461 tgattatatt gaagctcttt ttggtaagca gcataacatt ttgccgcaat ctcttcgtaa
    11521 taactgcctt gcatttttgc aaaatcctga ggggtctgca ggctgttttt tgcttgagag
    11581 attgttccta atatacttcg aggatcatat tttttcggat caatgttcag ttcttttagt
    11641 atccgtttca tcaaggtcag ctgttcagaa gaatcgatga ttgtaaagtt ccggttatac
    11701 ccgataaaat caacatctct tcttaaaata cgcacacaca tcgagtgaaa agtcgatacc
    11761 cagacatctt cgcctccaga tgcgagaatc gcattgacac gttctttcat ttcttttgca
    11821 gccttattgg taaaagtaat cgctaggatg ttccaaggat tgacttcttt ttcctcaatc
    11881 aaataggcaa tgcggtgtgt cagcactctc gtctttccac ttcctgcacc tgccattagc
    11941 agtaatggtc cgtcagtgtg aagaacagct tctttctgac gggggttcat cccgtttaat
    12001 aattcagctt tggatgacat taacaacatc ctttctttta tcaatcttgt taattatagc
    12061 attttcttgg ctttcattaa actagctagt tcaaaatcat ttgtttcttt ttatggattt
    12121 ttccttgtca attcgttgat ttcgcgctaa attaaaagaa agcgaggcgt ttgaatggaa
    12181 aaaaaaggaa aaatcaagaa attagataag actcagatat attttattct aaaaggaata
    12241 tttgttggac tgctgacagg aatcgtcgtt agcctgtttc gattatctat cgaaaagcta
    12301 agcgatttag ttcgaagtat ctatgaaatg agtcgggaac agccgattta cttaattggc
    12361 ctagctattt gctgcatcct tgcagcattt ttcgtgggtt atctggtcaa aaatgaaccg
    12421 gatatcaagg gaagcgggat tccacaagta gagggacagc ttcgcgggga attatcaatg
    12481 aactggttct ctgttttatg gaaaaagttc attggcggtg tactttcagt tggtgcgggc
    12541 ctttttctag gtagagaagg accttctata cagcttggcg catccattgg acaaggagct
    12601 ggacaattgt ttcggagtcc atcttcagaa gaaaagattt taatttctag cggtgcaagt
    12661 agcggacttg cagcagcatt caatgctcct atcgcaggat tactctttgt tttagaagaa
    12721 atccatcact ccttttctcc acttgtttgg ctgacatcat ttgcttcagc aatcactgct
    12781 aacttcgtct ctctttactt tttcggcttg agacctgttt tatatctagg tgaccttcct
    12841 tctttgccgt tgaaatatta tggttcctta gtgatcttag gagtacttct aggcatctta
    12901 ggttttattt atcagaaggt cttgctggct ttgccaaaat tttacaaccg attgccttta
    12961 ccagcttatt ttcacgggtt cgttcctttc ttgctgattt tgccaattgg ttatttatgg
    13021 cctagtactc taggcggagg caatcaaatc gttttagctt ttggacatgc ttccttgcca
    13081 atcattatga ttatcggttt gtttgttctt cgattcgttt tttccatgat ttcttacggt
    13141 ggtggtcttc caggggggat tttccttccg atattgacat tgggggcttt aatcggtagc
    13201 ttttatggaa acattatggt tgatttcggg atggatccaa tctacatcaa ggattttgtc
    13261 gttattgcca tggctggtta tttcacagcg attggaaaag caccgctgac agcaattatt
    13321 ttagtgacag aaatggttgg ctctcttaat catctgatgc ctttaggatt ggctgctctt
    13381 gttgcttata tcgtcaatga ccttctcggc ggtaatccaa tttacgaatc attattggaa
    13441 cgtctacttg gaaatactca gaccgcctca ctaaaaggtg caaggacaac ttttgatttt
    13501 ccagtaacgg cagagagttc tttagacgga ttaatggtac gagacttcaa ttggcctaaa
    13561 aatatgctgc taatctctat acgcagagga aatcaggaaa tcttgactca cggtgatacg
    13621 atcatgaatg taggagatat tttggttatc ttgactgatg aagcaaactt gccaagaatc
    13681 aagcaggaaa tcgaggctaa atcttctatc acaaatttga agcaatcatt ttaacgaaca
    13741 tggaaaaact attttacaac aactattcat ctacactact tgattctata tgattctata
    13801 aaaaaagaag agactgggaa aaaccagcct cttctttttt atttctatgc ttctgctgct
    13861 gcaggagctt ttctcaataa accataaatc acaccagata cgactgctcc gatagcaata
    13921 aacaccaaat acatgaccgg gtgactcagt aagaatacaa cgaaaatccc gccatgagga
    13981 gccagtaatt tgatcccgaa agctccgact aatccgccag ttaaagcaga accaataata
    14041 aaacttggaa tggcacgaat cgggtcagct gctgcaaatg gaatcgatcc ttccgtgaca
    14101 aacgatagtc ccatcacgat gttcgtcaaa ccagcatctt gatctgtttt gctgaattta
    14161 ttcttaaaca aacgtgtagc aacgaaaatt gccaatggag gtaccattcc tccagccata
    14221 acagaagcca tcacgacact tccgccagtt gcaacagtcg tagctaaagt tgctgtacca
    14281 aacacataag cggctttgtt gattggaccg cctaaatctg ccgccatcat tccagctaat
    14341 aatgcaccaa gtaaagcagc attcgaacca ctcatacttg tcaagaaacc gttcaatgca
    14401 tcattgattg ctttcatagg gacattgacc agcaacatca agaaaccagt aatcagtaag
    14461 ccaaacactg gataaaagag gatcgtttta attccatcca atgattttgg taagttcttg
    14521 aataatttac gtaaaaatac gatgacataa ccagctaaga atccaccaat cagtgcacct
    14581 aagaatcctg caccgcctgt attagctaat gcaccagcag caaatccaac gatcaaacca
    14641 gggcggtcac cgatacttga agcaataaat cctgctaata ccggcagcat gaaaccaaat
    14701 gcagcttgtc cgatttggtt gaaccagcta gcagcttgat tgtaattccc tagatttgat
    14761 aattgatcat gtggcacgcc aataaattgg tcgatcatga aagaaagcgc aatagcgatt
    14821 cctccaccaa taacgaaagg taacatgtgt gatacgccgt tcatcaaatc tttgtaaatt
    14881 ctttgtccga tagtaccatc cgcatttcct tcttctgaac tgctttcttt tccatcacca
    14941 tggaatactg gtgcagtacc actaagggct agattgatca gctcttctgg tttacggata
    15001 ccatcactga caggacgatt gatcagttct tttccgtcaa agcgattcat ctctactttt
    15061 ttgtctgctg caacgattac accttctgca cgggcgatgt cttcagcagt caatcggttt
    15121 ttgattcctt ctgaaccgtt cgtttcgact ttgatctcca cacccatttc tttcgctttt
    15181 ttcttcagag catcttctgc catgtaagtg tgagcgatcc ctgttggaca agctgtaacg
    15241 gcaacaacga attttctttc tggatgagca gaagcttctg cagcttgtgt ttcttgttct
    15301 gctttttctt ctgcttcttt ttgctcttca gctgtttgga acaagtcttg gacttcttct
    15361 ggtgtatttg cttcttttaa ttttcctacg aaatctgcat caatcagcaa tcgagatagt
    15421 gctgctaatg cttgcaaatg tgtgtcgttt gcgccttctg gagctgcgat catgaaaaac
    15481 agataagttg gctgtccgtc taaggcttca taatctactc ctttttggct ttttgcgaat
    15541 aaaactgttg cttctttaac tgcactgttt ttcgcgtgag gcatggcgat tccatcacca
    15601 agacctgtag aagtttgtgc ttcgcgggct agaattcctt ctttatatgt ttcgatatcg
    15661 gaaatccggc cggcatcaaa cattttttgg accatttcat cgattgcgcc ttttttatca
    15721 gtcgcttgca aatccatgat catcgcttct ttgaccatta aatccttgat attcatgatt
    15781 atcctccttg aaactaatca atttttgtga tttcaacttc tggcagcaac tcatcaatca
    15841 attcgcgagc tgccaagtca tcagaaaaag ctgttgcgct tccgcaagca actccccatt
    15901 taaaggcttc gattggatct ttcgtacggc tgtaagcccc aataaagccg gcaatcattg
    15961 aatcccctgc acctactgaa tttttcaatg ggcgaactaa tacattcgtt ttataagttc
    16021 cttgttccgt gaagagcaaa gcaccgtctc cagccattga gatcagcaca ttctgtgccc
    16081 cttcagctag caatttttta ccatacggaa tgatttcttc tacagattgg aaggttgtat
    16141 tgtaaagctc agctaattcg tgattgtttg gtttgatcaa caatggtttc ttaacaagtg
    16201 cattttttag gtcatcacct gttgtatcaa tcacgaaatc tgcacctttt tctcgaatga
    16261 tatcaatcag ttcttgataa aatccttttc gcaggctagc cggcgcgctt cctgacaata
    16321 cgacgatatc tccttgtcct actgttccta atgcttgctt caactcatcg atttcttcat
    16381 ctgaaataga tgggcccaaa ccgttaattt cagtttcagt atctgatttt agtttgatat
    16441 tgattcttgt atcttctttt acggttgtga acaaagactg gatttgttct ttttcaagcc
    16501 acttcgaaat aaattcgcca gtgaaccctc ctaaaaaccc tagtgctgta gattgagaat
    16561 cgatccgttt caaaatacgg gaaacattga tcccttttcc tccaggtaac ttgaagtctt
    16621 ccttcattcg gttcaaatcg cctagtttta acccatctac gcgtacgata taatcaattg
    16681 atggattaag tgttactgtg taaatcattg tattgcctcc ttaattgttg tcttttcacg
    16741 gaattcatta acgaattcta atggacaacg ctcggttatg atcgtggctg cttccaaagg
    16801 tgcaacagag gtgaaggaca actggttgaa tttggtatga tcaatcaata caaatgtctc
    16861 ttcggaattt ttcatggcta aacgtttaac agctgcctct tctgggtccg gagtcgtgaa
    16921 tccttgttca agatgggcac cgttcattcc cataaatgct ttattaaaat gaaaattttt
    16981 tagttgttct atcgcattag atcctaaaac agcattagtt gccagcttta tcgttccacc
    17041 taaaataatg gtagcaagct ccaaatcaac taaatttgcc gcatgttgaa cggaatttgt
    17101 cactactgtg atctgttttc cttgcagaaa aggaatcatt tctaacgtag tagagccagc
    17161 gtctaaataa atgacatctt cgtcattaat ggtttcagct gccgatttgg cgatttgctg
    17221 tttttcgtga acgtttttga gtgatttttc actcatattc tgctcaaaat tcaataattg
    17281 ctctttctta gctccgccat gaactctttc tagaaaccca ctattctcca gctcttgtaa
    17341 atcacgtctt atcgttgatt cagaagcatt aaaaaggttt acaagatctt gtgatttcac
    17401 gattttctgc tgttcaagca agtctaggat tttttgtcta cgttcctctg taagcatttt
    17461 cacgatcacc tcaatacaaa taatagcata ttttttttct actcgcaata taaatcagtc
    17521 aaaaacgttc ataaaaatta taaatcagtc aaaaaaaaat aaaaaaccag tcaaaaagac
    17581 tggtttttta tttttttgtc tatcatcaag ccgttttatc gttttttatt ttcgcttctt
    17641 gtttttttca tataaacaga agcaggtttt ctttccttat gaagaagata ataataaagt
    17701 cccatcccgc caaacatgac caaaaaaccg atgactgatc gaataggaaa attaggaaca
    17761 aaaaagagac agacggataa taatgatgcc ggaaccatta ttcgataata gagttgttta
    17821 tccattttct tcacctcata ttttttgtaa agtaatcaat tgttctaatt ctcggtaatc
    17881 taaaaattgc acacgcagat gctgctcacc tttcttgaca tcgaattgga tacttcgccg
    17941 ctttttcttt attcgtttga aggtaacatc agagagtcta tgtttgattt cctttaacgg
    18001 taattctgac agggattctg aaggaacata gattcttaag gataaaagat actcttgata
    18061 tttttcatct gttacctcta cttctagata ttccctaata taataacaat aagcttcgat
    18121 tccattatcc aaagcaacag tcggatcgat aactttatag cacatcatgc gcgagcgatt
    18181 ctcgtcaacc caaggaaaat acgcctgagc atttagtgtt ccgcgaacac gcctttcttt
    18241 ataatctcct tgttgaatat cataactttt tgttacaaaa aatgaagcat tctttttagg
    18301 aacaggttgc atttgtgcat tctgatactg gacagattgg atcgtatggt cagtgtaaat
    18361 gtattcaacg aatggcggtt tcaataaacc gacaccattg tcccaagcaa gatgatcaaa
    18421 cccaagctgg tccctaaaat aggattgaaa catttgattg tttggctttt gccagttaaa
    18481 ggggtctcga taaagctgga tacatatgac ttcgttttct aattccttca ctttttctac
    18541 ttgttggatg tcgagaaaaa tcggtttagg gatgatttga tgataatagc gactataata
    18601 catttcccaa cacgaagtga aacataaacc ttggtaaaaa agatcgtatc ctggaaattg
    18661 gttgcaatcg actactactt ccccgttttg ttgtttcatt ttaggtaaat gttcgatttc
    18721 atcctttttc tcaaacaata gccgttgaga gatctgtttt gtattgtgat agataaactc
    18781 gctataagct cgcatatagg cgaacacccc atgagcaatc atttttttct gaatgaagtc
    18841 tcgaatcaaa aattgatttt ctttgaaaac agagatagac aatacatttt ttatcaataa
    18901 ttgattatct tccaagcgaa aactacattc gttctcgtca tttcctacaa ctaaccaact
    18961 ttcgtctctt accgatgcta gcattatttt ttcttgaagc tttcgaagat catacggatc
    19021 cgcttgttgt gtgaaagagc ggataaatgt tggctggatc aacagacgtg tcaaaatagc
    19081 ccaatcgctt atttcctgtt ttttttcttc ttcatttaag tctgtgagct ggatagtcat
    19141 caataattta tcttcgccaa tcattttctc acctctttaa aatcttttta taatttaatt
    19201 ataaccaacg ataaagaaaa actaaaactt tatgtcttag aaaccaattt ggacgattac
    19261 atgatagact aagtaaaaag aaacaaagag gtgtttacaa tggggttgaa atttgaacgt
    19321 acagataatt tagataaact tttcgaatca tttgctgtcg atccggaaaa aacagttgca
    19381 ccaaaagaag agatcgatcg atttctaaaa ccacagccac aaaatgaaaa gaaaaaagac
    19441 aagaaacaaa agtaagttaa gctcaaaaaa agacttacca gccaaacttt tatgaaagag
    19501 tttgactggt aagtcttttt tattcttttt atgattcaat tgcttgttcg attgctgccc
    19561 acgcttcttc ttttccctgt tttgtaacgg aagaaaagag aataaacgtg tcattgcgat
    19621 caaaatccaa cgcttttttg atttcgctct catgcttgtt ccatttacca cgagggatct
    19681 tatctgcctt agtggcaaca acaattacag ggatatcata atacttcaaa aattgataca
    19741 tctggatatc ttcttgagaa ggcttatgac gcaaatctac taaagaaacg accgcacgta
    19801 gctggtctct agtcgttaaa tatgtctcga tcatctttcc ccattttgct cgctcagttt
    19861 tggatacttt tgcataccca tatcccggaa catcgacaaa atgtaatgcg ttctcgatca
    19921 aataaaaatt caatgtctgg gtctttcctg gcttaccgga agtacgggct aaattctttc
    19981 gatcaatcaa tgtgttgata aaagaagatt tccctacatt tgatcgtcct gccagagcta
    20041 tttctggcaa ttgtgtatct ggatattgtt ttggtgccac tgcacttatg ataatttcag
    20101 cttgatggac tttcatggtc tctctcctta acaaggtgat gaataaagca agcggctgtc
    20161 gcaaaagcca aaatgacttt tacggcagcc tcttttcatc aacccgcttt tttctctgaa
    20221 tctttataga ttactgttgg ctcaccagac aattcagctg cttcttttgt gataatcact
    20281 ttttcgatgt tttcatttga agggatatca aacatcacat ccatcatgat ttcttcaatg
    20341 atggaacgaa gtcctcgagc accggtattg cgttcgattg cttttttcgc aatggctctc
    20401 aacgcttcag gttcgaattc aagttcggta ttatctagag aaagaagttt ttgatattgt
    20461 ttgactagcg catttttcgg ctcagtcaag atccgtacca gatcatctgt tgtcaatttc
    20521 tctaaagctg ccataacagg taatcgtccg ataaactcag ggatcaatcc gaattttagt
    20581 aaatcttcag gaatgatatg ctgcatgaca ctttcgtctt cagaaagttt ttggttttgt
    20641 gtaccaaatc caatcgtctt ttcgcccata cgattcttca caatcgtttc gatgccatca
    20701 aatgcaccac ctacgatgaa caatatgttc gtcgtatcaa tctgaataaa ttcttgatgt
    20761 ggatgtttac gtcctccctg aggtggcacg cttgcaacag ttccttctag aattttcagc
    20821 aaggcttgtt ggacaccttc gccggaaacg tcacgagtaa tagatacatt ttcgctttta
    20881 cgcgcaattt tatcgatctc atcaatatag atgatccctt tttctgcacg ttccacattg
    20941 taatcagcag attgaagcaa tttaagcaaa atgttttcta catcttcccc cacataacca
    21001 gcttctgtca aacttgtcgc atctgcgatt gcaaaaggta cattcaatgt ttttgctaat
    21061 gtttgtgcta aaaatgtttt accagaccct gtaggtccga tcaagcagat attgcttttt
    21121 tgcagttcaa catcatctgt gttttcttgt gcttgctgat tgacacgttt atagtgattg
    21181 tatacggcaa cagctaacga gcgttttgcg cgatcttgtc caatcacgta gttattcaat
    21241 acttcaagaa tttctagtgg tttagggact tcagtgaatt cgcgtacagc ttcttcataa
    21301 aattcttcat caatgatttc tttacatagg tcgatacatt cattacagat gtagacccct
    21361 ggaccagcta cgatcttttt cacttcttct tgggttttcc cgcaaaaaga acagcgtacg
    21421 gtctcattgc tacttggatt gtcatacatg gtcttcaccc cttaacttgc aaaaagtatg
    21481 caagaaacta tcaaagactt tacagtcttt tactatcata acatagttta gtaaaaagaa
    21541 aaagtgttta acaaaggatc aactgaccta tacctttgca ttttttactc ttaacaaaaa
    21601 ataaggaact gtgacaaaat cgtgtcacag tcccttattt cgaataaaca attttctaga
    21661 agtagcttct tcagaaatga tttattactt gatttctatt ctttcgcttc tgtcgctgta
    21721 cctgtgatca attcgattgc ttttttcatt gtcacatcat gttccagcat atcttctgtc
    21781 aacacgcgtt tgatttgttc gattggcatg ttgtatgttt ctgataattc ttggatttct
    21841 gcattgattt cttcttctgt tgcttgaaga ttttcagcag ctgcaattgc ttcgatcaca
    21901 agatttgttc ttgtgcgcat ttctgcttca ccttcgaatt gtttatgcaa gtcttcttct
    21961 gtagaaccag ttaattgata gtacatttca ggagagatac cttgacgttg catgttgttc
    22021 aagaattcgt ccattgaacg atgtacttca tcatgaacca tcacgtgtgg caattcaacg
    22081 atttctgcgt tttcaacagc catacggatc gctgcttcat cttttgcatc gtccgctgct
    22141 ttttcttttg tttctactaa ttctttgcga tatttttctt tcaattcgtc taatgttgat
    22201 acttcgtcat caacatcttt tgcgaattcg tcatctaatt caggcacttc ttttgctttt
    22261 acttcatgga ttgtcacttt gaagactgct tcttttccag caagatcttc tgcttgataa
    22321 tcttctggga aagttacatt tacatctaga ctttcacctg atttatgacc aactaattga
    22381 tcttcaaaac ctggaatgaa tgagcctgaa cctaattcaa gtgagtaatt ttcgcctttt
    22441 ccgccatcaa acgcttcgcc atctacaaag ccttcgaagt cgattactac tgtatcgcca
    22501 ttttcagctg cagcatcttc tttgataact aattcagctt gtgcttcttg ttcacgtttg
    22561 atacgtgcat ccacgtcagc atctgttact tcacggtctt gtttttcaac aactagatct
    22621 ttatattgtc ctaattttac ttctggtttc actgttactt cagcagtgat tacccagtct
    22681 tgaccttttt ccatgctttc aacatcgatt tttggttgag aaacaggatc gatgccagct
    22741 tctttgacag cagcttcata agcttctggt aatacagcat tcaatgcatc ttcatataaa
    22801 gcttcttcac cgtacatacg gttgaatact tgacgagaaa ctttcccttt acggaatcca
    22861 ggtacgttca agcttttttt cactttatta aacgcagttg ttaatccttt ttggatttcg
    22921 tcttgcgcaa tcgtaaattt taaaacacca tcattcgtgc cttttttttc aaatgttgca
    22981 gtcatttaat tccctccgag tttagaaatt cattttatac atcaaaaacg tttcatgcat
    23041 acctttaaat agtacactaa ccctttcaaa ttgtaaacat attgaatgga tagaatcctt
    23101 gtttttcata acatttttat ctttttcact gataaaatct aatggaagaa gatcatttgc
    23161 tgcaaaattt tttctctttt ttttaaaaaa tcttgttttt catcttctgt accttcatac
    23221 atcgtttttc ctgtacacgc ctctacagtc agttgaaacc agcgcttcgg gtcttcgatg
    23281 tatgtatcat agacaggata aacaattgca gattgcaacg tgaattcttc atttaacacg
    23341 gaactaagca gcgcatcttc attaccgtaa cgatctgaaa atacacataa ttgctggtaa
    23401 cttgcctgct gctgcggctt tggcagatct tttggtatga ctttgactaa cttttcttca
    23461 atcgtcaaat agaaaacagg atggcattcg ttcaattgcc taagtgtctc cagtaatttt
    23521 gcccttgcta aaatatggac ctctggcgat acaataagtt tactcgctgt ttggataaac
    23581 tcatgataag gaagctgttc gatctcatca atagacgcaa gctgacgtgc aggttccaac
    23641 gaaggaagtt cctccgattt attgtggagt gccttgatct tctgcctctc catttgtccg
    23701 gataattctt ctgcaaattg gattttttca atgacccgct gctcgaaagc ttcataaaaa
    23761 tctgtacttg ctatcaattt tctagcatat aaaaaatctt gtgtactgat taacaagtca
    23821 aaataaaatt cagcaaactc ctcattttga agatactgtt tttcatgcaa gagtgcttgc
    23881 ttcaatgctt cttttttctc gtccaactcg taaagacaaa aaacaagcag gctattagcc
    23941 tcaaagcttt ctttcaactc gtaagctcgt tgaaataatt cttttgcctg ctgatattgc
    24001 tggtttgcta gtgcttgttg tgctactcgc attaaattct catatttttc tggaaaatca
    24061 atcggcttca cgcagtgtcc tcctcttgct catgttcagc tcatgttcaa tagtagcaaa
    24121 acaaaaaaag catgacaatt aaaaataact gaagcaccct ctaaaagcta gattttagat
    24181 ctgactttta gaaggtgctt tcaactgtta ctgcttttta ttgctcatca gctgttgact
    24241 tcctggttga atgctttgat cagattgatc gtaacaacac ttgtattagt gaaaaaatcg
    24301 cctagatgtt gctttttctg attaaagatt gctggaagat accctaacat cagcaaagga
    24361 aattttagaa tgaaccggca agcaccttct ctaactaaaa cggttgacca gctaagttct
    24421 tcttcatcaa agctgatgac tcgaatacca aagatcattt tcccaatcgt ttgaccatga
    24481 ttgaatttcg tcaataaaat aaaatacgct agataaataa gtaaggctgt gaatccgtag
    24541 atactcagat aagaatctga aaaattaagc caacctagat ttttggcaat accaagtgtt
    24601 aaacgcgtaa tagctccgat acacaaaaga tcgaccaaga aagcaaacat tcggataaaa
    24661 aaacctgcat agaaatactt aggaaaatca tttgcttttg gcggttcatt tcttgtgtcc
    24721 gtatactttt tccatttacg ttgtttcttt ttgatttctt cgggcgtcaa aggctctctt
    24781 tcaaaagagc tcagtacctt ttgggaaaag ttggagtcag gtcctacagc aggctgctgt
    24841 tgcttatctt tttcttgttc cttattttca tttgaaagct ctgacatgct tactcacctc
    24901 cgtaataata cattgctttt ggtgattccg ctgtccctag attctcaaga acggataaga
    24961 tctgactagt ctcagaaggc ttcaatccct ggaattctgc tagtttactt cctaaccatg
    25021 tactagcaaa cccagatggc gtggaagaat attctactag ttccgcatct tcaagctgtt
    25081 gttcctttcg catagctttt aaagcatctt caggaaatcc caattcatcg actaaaccta
    25141 cttcttttgc ttggacacca tcataaatcc ttccatcagc aatttttttg acttcttctt
    25201 cagatttatt ccgtccctcg ctgacgattg ctacaaaacg actatatgcg ctgtcgatat
    25261 atgcttgaag caccgcttct tcttcttttg tctctggtcg cgttcctgaa cccatatctt
    25321 tcaacgctcc gcttttaact gttgtatctt ccacacccag tttttctaga agtcctgaat
    25381 aattcaatcc tgacatgatc acaccaattg agccggtcac tgtttcttct gttgcaaaga
    25441 ttttatctgc ttgagcagaa atatagtagc caccgctagc tgccatgttt ttcatgctga
    25501 catacattgg gatatcatgt tctttacgaa tcgtatccaa tttcttagcg atttccgcac
    25561 tttcgtagac accgccacca ggagaattta cttctaaaaa gatacctttg actgttggat
    25621 cttcttcaat cgccttcagt tgttccataa aatttttatg attgtaacct tcagaagcaa
    25681 ataaacctga agaccctccc gcagcaatcg ttccatctac tgataatttt acgatttttt
    25741 gcgtactgtc tccctcttcc aacacatttg aagctagttc ttcatcccca tataaccaac
    25801 tgttcaatcc ctcaatttga tcgttttcct gaggcttagt caaactagct gaaactaacg
    25861 aaaccacaaa taatcctgca gcaatcagaa ctgctgccca acgtctctta ttcattcttt
    25921 ctcctttcta atacaagttt tttatgacga tcacagtttc tgactgctca gaagtgtcga
    25981 accgctctgc tttttcttcc cgcgcatacc cggaaaacga ctcttttgtc aattcatctt
    26041 ctaccgtaaa ctcacgagct tcagcaactt ccaaggcatc aggtgcgatt tcttggatgc
    26101 tatcccagcc agtatctaaa aatattttcg tatcatctga tacaccagct gatgcttcga
    26161 atttggacaa ttgttttttt aacattccca gtgtcatgat ctcaattttt gaatccattt
    26221 ttactcctcc tctatccgat tactttctat tgtaccgaac tcctgaaaaa gatgatagat
    26281 aaaaacccat tattttttga cataaaaaga gcatttgaca aagcatttga tatataccaa
    26341 tttatttttt attctttttt tgttgacaag aacattcgca aaaggttatg attttaaatg
    26401 tttccagata aaaaacaaaa gaggaggata gttatacctt ttaggatagc gccaggtctg
    26461 tgtaaacaga tgacgaggag agggtttatc gaaagattcg gcggatggcc ctagggactg
    26521 cactctacag aaaatccaca aaaaatatct gcaaagataa aacaaaagga ttttcctgca
    26581 gctggaagca ttcatacgaa ttcattatag aaagtaggat gttttttatg tgcggaattg
    26641 ttggaatgat tggattaaaa aatgtaacac caggattgat tgacggtcta gaaaaattag
    26701 aataccgtgg ttatgattca gctggtatct ttgtctcaga tggcacaact gattatctgg
    26761 tcaaagcaca aggcagaatt caaaatttga aaaacaaaat aaatattgat actacaggaa
    26821 atataggtat tggtcataca cgctgggcaa ctcacgggca accttctgaa gaaaacgccc
    26881 atccacatac ttcccaaagt ggacgcttcg tgctcgttca caatggcgta atcgaaaact
    26941 ttgaagaact taaaattgct tatttagcaa atgaccactt tattggtgag accgatactg
    27001 agattatcgc tcatctaatt gaaactttcg cgaaggatac tacaacgcaa gaggcgtttt
    27061 taaaagcttt gcgggtcatc aaaggttctt acgctttcgc tttgattgat cggacagcac
    27121 ctgatgttat ttacgtcgct aaaaataaaa gtccactatt aatcggactc ggcgatggat
    27181 tcaatgtgat tgccagcgat gcaatggcta tgcttgctca tacgaaggaa ttcgttgaga
    27241 ttgaagatga agagatggtt accgtcacat cagaaaaagt tattattcaa aattttgcag
    27301 gtgacatcgt agaacgttct tcattcgaag cacaagtaga ttcttcagat atcgagaaag
    27361 gtacctatcc tttttatatg ctgaaagaaa tcgacgaaca gcctattatc atgcgtcgta
    27421 tcgctcaaaa atacgtaaca gaagacaatc gtgtctctct tgatcaaaag ctggtcgata
    27481 cattgtctga tagcgatcga atctatatcg ttgcttgcgg aactagttac catgcggggt
    27541 tagcaggtaa acagactctg gaaaaattga cacagatccc tgttgaggtt catttagcca
    27601 gtgaatttgg ctataacaca cctcttcttt caaaaaaacc attctttatt ttcttgagtc
    27661 aaagtggcga aacagcagat agccgccaag ttctggtaaa aatcaaccgt ctcggctacc
    27721 cttcacttac gattacaaat gttgctggtt ctactctttc gcgggaagca tcctttacac
    27781 tgctattgca tgctggaccc gaaatcgcag tagcatcaac gaaagcttat actgcacaaa
    27841 tagctgtact aactttatta gccaaagcta tcggtgataa aaaagaattt gaaacttctc
    27901 ttgtattcga tatcactcat gaattgagtt tagttgctaa tgcgatggaa tcggtcatcg
    27961 cccaaaaaga ttatttagaa gagttggcag aagtatatct cagcgataca agaaatgcct
    28021 tttacattgg acgtggcgct gactacaacg tttctcttga agctgctttg aaattgaaag
    28081 aaatcagtta tatccaggca gaaggttttg ccgctggcga attgaaacat ggaacgattg
    28141 ctttgatcga agaaggaaca ccagtgattg gaatcatcag tgaagaagtg actggtgcgc
    28201 atactcgagg aaacctgaaa gaagtcgaaa gccgtggcgc taaaacacta gttattgtct
    28261 cagagggatt agaaaaggaa agtgaccagc tggtgctacc aactgtccac ccttatctaa
    28321 ctactctaac cacagtcgta ccgactcaat tacttgcata ttacgcaacg ctattacggg
    28381 gattcgacgt tgacaaacca cgtaatttag cgaaatcagt gacagtagaa taataaaaga
    28441 acaaggacca gtgtcagaca tctgacactg gtccttgttc ttttattttt tatgaaaata
    28501 tctcttaatc cagtccgatt tcttctttga ctactgccgc gattttatcg acatagtaat
    28561 taactttctc atcagtaggc gcttctgcca tcactcgaag taatggttct gtaccggaag
    28621 gacgaactag gattcttcct tctgatccca tttcagcttc tgcctcttca atcacttttt
    28681 tgattgccgg aacatccatc gcaccgtttt tatcacttac tcggatattc actaattttt
    28741 gcggatagat tgttacctct gctgcaagct ctgataattt cttacctgtc tgtttcatga
    28801 tattcagtaa ctgaattcct gaaagcatcc catctccagt tgtgttaaaa tctaagaaaa
    28861 tcatgtgccc agactgctcg ccgccaaagt tataatcgtt tttgcgcatt tcttctacaa
    28921 cgtaacgatc accaacttgt gtaacgacat ctttcaaacc gattccttcc accgctttgt
    28981 ggaatcctaa gttactcatg acagtcgtaa cgatcgtatc ttttttcaat cgattttttt
    29041 ctgccaagta tttagcacag ataaacatga ttttatcacc gtcgacaatg tttccaagtt
    29101 catccaccgc aataatacga tcgccatcgc catcgaaagc tagtccagca tctgctcctt
    29161 tttcaacgac catctcagct aaacgttctg gatgggtaga gcctacgcca tcattgatat
    29221 ttaacccgtc tggatttgtg cccatggtat aaaaatctgt ttcaagatcg gcaaacaaac
    29281 gattaacact tgttgaagtt gctccgtttg ctgcatcgac acaaatggtc aaaccggaaa
    29341 ggtcaccgtt gatcgtttgt acaaggaatt gagaatattt caataagcct tctggaaatt
    29401 cttctacagt tcccaatcct tcagctgatg gacgaggcaa ttcatccact tcagcatcta
    29461 ataatgcttc aatttctagc tcttggtcat ctactagttt aaaaccatct gcaccgaaaa
    29521 atttgattcc attatcttgt gctggattat gagaagcaga aatcatgacc cctgcgcttg
    29581 ctttttgcaa acgtgtcaga taagcaacac ctggtgtaga gatcacacct agctggaaca
    29641 cttcgatacc caccgataaa agtcctgaaa tcagcgcttc ttctaacatt tgacctgaga
    29701 ttcttgtatc ccgccctact aacacgcgtg ggcgttttcc ttcttcttca tgctggctta
    29761 ggacataacc tccgcaccgt cccagcttaa atgcaagttc tggcgtaagt tccttgttgg
    29821 cttcaccgcg tacgccgtct gttccaaaat atttacccat agttgtaaaa ttactccttt
    29881 aacttaattt tctgaggaat ttgttctgct catttgtgtt gattcgcttg tttctgatgt
    29941 ttcttctgta cttgtggtcg agctgcttgt cccagattcc gaactggaag aaatggttgt
    30001 tgtcgatggt tccgttcctg aatcagcaga tgaaatactt ctactcgtac ttgtattttc
    30061 ttgtgtccga ctgctcgtcg aagcagagct actgcttgac gtttgcaaca cgggtgtaat
    30121 ctccacacta actgattcgg gcttgatact taacccctct tctactggta tttcaatttt
    30181 acgagttgtt cgatgattga tacctgagac attgatcggt acagcaatac tgtcaccgat
    30241 cgatgcaagc aaatcgctag agccgcttac ttctgcttcc agttttgaaa aactgaaatc
    30301 atagctttct accccggcag ctggtgttcc ttgctgtatc ccataaagtc gtaccgactt
    30361 gcttggcttg atcgaatcag ctctcactgt tacttgcact ggatcagaga cgatacttaa
    30421 tacttcccct gctgcattca gcgcctgtat tgggacctca ctattaatcc catcatctgt
    30481 gaccatcgaa gcgtccacat cagctactac tcgtttgatt tctgccaatg ttttgtcacc
    30541 tgtagtgata gagacttttt taggactaac ggaagtatca tcaagttcat atccagatgg
    30601 tgtgaacgtt gaaggaatca acgtttccac atcgaaatct tttattacct ttttctcaac
    30661 cgttactgta atagtcgatg gttcgatctt tgctgtgaca gcactgctca aattttgaat
    30721 ttttagcgga acctcatgtg ttccttcgcc cagcttagtg atatctgctg tgacgcggaa
    30781 catccgtgta tcttcgtttg cctctgcatt caactgaact cggtttgcac tggaaagttt
    30841 gacagtcact gcattttcat atccatggat atagtagcgc tttgaatcat aagaaatctt
    30901 gattggcacc ccagtaactg tttgattata cgcttcattt ccagaaagcg tatttggaac
    30961 ggactggcca tttgcattga agtacagcgc aatactgaac agcaatgcaa taaaactgta
    31021 aagaatgctt gtccgtttct cttttgagat catttgcgat cccctccctt agtcataccg
    31081 tctaggaaag attgaatcac gtttttcttg ttttttgttt tctcttcagc aatcagttct
    31141 ctacgcaaaa ttgccaaata ttcttgctgg gataagccag ccatcagttc attatctaat
    31201 gtaatgctga catccccagt ttcttcagag acaacgatgg taatcgcgtc gctgacttcg
    31261 cttataccga cagctgcccg gtgtcgagtc ccgaattctt ttggaatcaa catactttct
    31321 gatagaggaa gataggcgct tgctactgcg atctttcctt cttttacaat aactgcaccg
    31381 tcatgcagcg gtgtattagg aataaagata ttgatcagca gctctccagt gatatctgca
    31441 tctaatgcaa ttcctgtctc aatatactca tctaatccag tatgccgttc gatagtaatt
    31501 agcgcaccga tttttcgctt ggacatatat tggatggctt tgtcaaaaga caagatcatc
    31561 ttctcatctt cctgctgttc gctttttgat tgtttgaaaa gcgaactgcg tccaagatgt
    31621 tccagtcctc tccggatttc cggctgaaaa atcacgatag cagctatcac cccgtacgta
    31681 atgacttggt tcatcagcca agacaaggtg tgcagaccaa tgatttccgc tacgattcgg
    31741 ataacaataa aaatagctac acctttcaga agctgaatcg cttttgtgcc tcgcagcagt
    31801 tggatcagtt tgtatacaag ataccagaca accagaatat caagtatgtt caccaaaaaa
    31861 ttcagagaga acaagtcacc ggaaaacatt tgctgccaat agtccaaatt aaaaagttcc
    31921 ccgatttgaa atgacataaa aagcctctct tctcttttga gatttcctct ctagtatatc
    31981 atatgtttta attttagagg ctgatctgct cgtatttttc ttgagaaaaa cgcataattt
    32041 tcaggaaaat tgcaagtatg cgttttattt ttctctgcaa taaatatttt taaacttcag
    32101 agaattattc attatatttt ttctgttatt tttccttttt ttcttttgta gcttgttcca
    32161 tttttttacg tcgttctgct ctggcagctc ttctttcttc tgcttcaagg tccgtcgttt
    32221 tttcttttct agtcttaacg ggtacatcga tagattcctc tggttcaagt cgtttcttga
    32281 ttttttctaa ttctcccgtc agtgcagcat agttgtaaaa acgtgtttta gcgtcttgga
    32341 tagtcttttc aaataaagct tttgcaagat ttggatttgc gttctctagc gaggaaaagc
    32401 gaacttgttt cctcatgaaa tcaggcatag cagagaaatc aggttttttg tagtccaaag
    32461 tcattgggtt cttgtttttt tcgaccattg cagggttata tcgatataat gaccaatagc
    32521 cagaagaaac agcttctttt gcttctttaa gcgattcttt cattccacca agcaaaccat
    32581 gtgtgataca aggcgtatag gcaatgatta aagaaggtcc aggatacttt tctgcttctt
    32641 caaaagcctt tatcgtctgc atttgattgg cacctgaagc aatttgcgcc acataaacat
    32701 ttccatatgt catcgccatc attcctagat ctttctttga agcatacttt ccactagccg
    32761 aaaatttagc gattgccgaa gctggtgttg cttttgaggt ttgaccgcca gtattggaat
    32821 agacttcgtt atccaaaaca agaatattca cgtctgctcc gctggcaagt acatgatcga
    32881 tcccgccaaa accaatgtcg taagcccatc cgtctccacc aatcatccat tggctgtttt
    32941 ttacgaatag atcctttctc ttgtatatat cctccagttc agggacaatt gatcgttcgc
    33001 tttcgatcgc agcttgcagt tttgctgctc tttgctgcgt tccttcgctc tcaaacatgt
    33061 gtaccaacca gtcttccatc aattctcgta gttcttctga tacttttggc aatacttctt
    33121 tgattgaact tgccaaagcc tcgcgacgag tctgtgccgc tagaagcata ccataaccaa
    33181 attcagcatt atcttccaat aaagaatttg accatgcagg cccttgacca cattcattgg
    33241 tcgtataagg agaagcagga gaagcaccgc cccaaataga agaacagcct gtcgcattcg
    33301 cgatcatcat tcgatcaccg aacatttgtg tcaataattt tacataaggt gtttcgccac
    33361 agcctgagca agcgcccgag aattcaagaa gcggttgatt gaattgtgta gacaagacag
    33421 tattctttcc tttgactgga tttgcttttt gtcttaatgt cattgcaaaa gcccaattca
    33481 cagcttgttc tttctgttct tcataaggct tcatcactaa agctttttct tttgcgggac
    33541 aagcctctac acacagacca caacctgtac agtcttccac tgaaacttgg atacggtatt
    33601 ttacgccgtc tgcgcctcgc atttctctca cgataaatcc ttctggcgct tcttccattt
    33661 cctcttcatc tgctaaaaat ggacggatgg cagcatgagg acaaacaaag gcacattcat
    33721 tacacatcgt acagcgatca ctaatccact caggtacttc taaagcgacg ccccgctttt
    33781 ccatagaagc cgttcctaaa ggcatttcgc cagttgtcat tccatttgct acaagtgtac
    33841 caacagagag ttgatttcct tcctgtgcat tcactggttc aagtatttct tgtatataac
    33901 cagtgttcct tttattgatt ttttcaggca tctccaacgt tctccaatgg tctgggatcg
    33961 cgacttgatg aagcagttcg gtcgtccgat cgattgcttg tatgtttttt tctacgatag
    34021 ccattgattt atgtccatag cttttcaggg cttcttcttt tagcagcgga agcacctcat
    34081 caaaaggaat gatgtcagct aatttaaaaa aagccgtttc cattgccgta ttgatccggc
    34141 gtcccaaacc gacttcttgg gccaatctca tggcattgat cgtatagaat ctaatgttat
    34201 tttcagccaa gtaacgtttt aattttaagg gcagatgcgt ttctaattgt tcgtcgctcc
    34261 aaagagtatt caataaaaaa atgccacctg gcttcaatcc tttgaccaag tcatagctat
    34321 gcaggtaagc tggtgtgtga caggcaacaa ggtccgcatg ctcgacaaga tacgctgaac
    34381 gtatcggcgt atctccaaaa cgaagatgag agacagtcag accgccagat tttttcgaat
    34441 cataataaaa atagccttgc gcatatttat cggtgtgatc tccaataatc ttgatagctg
    34501 atttatttgc tccaactgtt ccgtcagatc caaatcccca aaacttggcc tggaatgttt
    34561 gcggttctgt caaatccaga ctttcctttg gttccaaaga ttgataagtc acatcatcca
    34621 cgatccctat ggtaaaccgt tttttgcgta tgcttgggtc ttttttcaat tcatcaaaaa
    34681 ctgctgagat ttggtcggga gtcacatctt tagaccccaa tccgtagcga ccaccaataa
    34741 cagccgggcg aagttcagaa tcatataacg cactttgaac gtctaataag agaggttctc
    34801 cacctgcccc tggttcttta cttcgatcaa gaacagcaat tgtttttact gtatgaggca
    34861 acttttgcac gaatgtttcc actggaaaag gacgataaag atggatattc aaaaaacctg
    34921 ttttccgtcc gtttgcattc aagtaatcaa tcgtttgttc aatcgtctgt gcaactgatc
    34981 ccatagaaac aatgacttct tccgcatcgt ctgctccgta ataagtcacc aagtcataag
    35041 aagttcctcg taatcggttg atctctttca tatatttttg aacgatttca ggtaatttat
    35101 cataataggg gttgatcgtt tcccgttgtt gaaaatggat gtctggattc tggttcgtac
    35161 cactaacagt cggatgattg ggattcatcc ctcgtttacg aaatgaagct aatttctcct
    35221 gattgatcag aggagctaat tcttcatatt ccaaaacttc gattttttga atctcgtgac
    35281 tcgtacggaa tccatcaaag aaattcaaaa aaggtacttc tccttcaata gaagcaagat
    35341 gggcaacagg tgctaaatcc atcacttctt gtacactgct ttcagctaac atagcaaatc
    35401 ctgtttgtct tgcagccatc acgtctccgt gatctccgaa aatattcaac gcatttgtcg
    35461 tcactgcccg actagcaaca tggaaaacac ttggcagtag ttccccagca atcttgtaca
    35521 tattcggtat catcaaaagc agcccttgag aagcggtata tgtagttgtc agtgcaccag
    35581 ttttcaatga tccatggacc acaccagctg ctcctgcttc ggattgcatt tctactactt
    35641 ttactggttg accgaataca ttttcttttc ctgctgctga ccattgatct actaattcag
    35701 ccatggtcga actgggtgtt attggatata ttgcagcaat ctcggtaaat gcataagaaa
    35761 tatatgcggc tgccgtattg ccatccattg ttttcatttt tcgcatgatt catcatcctt
    35821 gttcatattc tctcttatta tctgttccat tataacgtaa aacattccat tttttctttt
    35881 atttataaaa atatttcaca tatgtctatt tgaattggtc atctaatttt tagacaaaca
    35941 gtctaaatcg ttgccttttt ttgtcagaca tgtataaact ataagcgaaa gaggtgtttt
    36001 aacatggata tatcagtgat tgatgcaacg aaaacaaata cacaaaaagg aatcctttat
    36061 ggttcatcga atgctcctaa aaaaatggtc gaattcatca acctatcttg tccgtattgc
    36121 cgacaatggt ttgaagaatc ttatgagcta ttagaagaag cagtccaaag cgggcaattg
    36181 cagcgagtga tcaaattgtt cgataaagaa aaagaaagct tgcagcgagg aaacgtgatg
    36241 caccggtatc tcacaatcag cgatggtcaa aaagcgatca aagagatcaa acaaatattc
    36301 gatactcaag acgagtggaa acatctttct ctccaagaag tagcggattt tgcagtagat
    36361 aaactaaagc tgacagaaca aaaagacgaa cagctgagcc aagctgtcat caacgaagct
    36421 gagcaagcac atattcgatt tgttccgact gtcatcttgg gtaaagaaat ctttgatgaa
    36481 tctatttcaa tagaggaatt aaaagaattg atccaaacaa aataaaaaaa taaggagcgt
    36541 aggacaaaag taaagaacac ttttgtctca cgctctaaac acctataaac ggcggaagca
    36601 gaagcaactc cttcttattt cttggagtta aacgcttctg tcccagcctc cttattccga
    36661 ataaatggtg tccaaaagct tgcatctgcc agattatctc atttcttatg acaagctgag
    36721 caaattatct ccaccaaata atttattcat atttttgact tgcgcatact aactaattct
    36781 taaattttca tacaaaattt ctcaaattcc cttaacgagt aatatttatg tacggtgatt
    36841 cacactagta tcccaatcac tttttaagtt atcccgattt caattctact tcaaaaatag
    36901 ttgcttttat gtatgctgtt agttgattag atggtcgttt gttgagctag gtcgtttatt
    36961 ttaaacaagt tgtttcttta gcttatacct atctcgtcta ccaaacgcaa gatggcaaac
    37021 acaaatgcat acgcatctgt atttgccact ttgattcgta taaagctaac aactcctaac
    37081 tcctctcatt taatagttgc tctctttttt attcagatac atttttgctg atcacagcct
    37141 agtatttcaa tggttttgaa cactaaacca aattgttcca ttacaacatt cactggctga
    37201 tgaaatggat taatagattt cgattattca aagcgatttt ctattggtgg attgtgttcc
    37261 caactaacaa gacggattcc aaagtcatta tataactctt caagagttaa ccctttcctg
    37321 ttcccaatag atgtatctct cccacgctcg atataaaata tggagtttcg aatgccaaga
    37381 cctggcaaat ctttccttgt tacagttaca ctgctactaa cttccccata taaaatccag
    37441 ctcctccaaa atgataatat atttttcgac catcatctaa ctcatgtaac aataattgtt
    37501 gtgccctctt gtcactcgcc aaatgcaaac gccacgtgcg ataccatgcc atgctacctg
    37561 catctgcaga aaatccatca aataatgcaa caattgtatc ttctatcaca atcctttccc
    37621 catctacttc atattctaat ctaaaatcaa actcccctcg ggtaatcgca ggtcttggtg
    37681 gcttaggacc caatattatt ctacttaaaa ccgcattcgt tcccagatga aacacagcta
    37741 taacaacaat aatgattaat attttgtttc tatttgataa atttctcatt attcgcaaat
    37801 cacctctaca ttctcacata ctagttcgta atactccttt aatatcaaaa tatctactga
    37861 tcgtaataat attcgctatg aattcctact actcgtataa gaaaggccaa atcatcagct
    37921 tttacttgct gatgatttgg cttgtttcta tttatcgtaa ttgttttcta atcaccaaaa
    37981 ttgttaacaa tcggttcaga tatttcccaa ttcactagcc ttatattata ttgatttaac
    38041 aattcttctt catttattgt attttgatca agcggaccaa attgttctaa taattctttg
    38101 tcatgtaaaa tatcccttct agtaatgcct ctttcccttt caatgaaaga agcactagga
    38161 aaagtatgat tataaccatt caacctatct cccatgtaat aattagcagg tcctggaaaa
    38221 taaaatattt gcttattatc actaacttct aacagcaaca ctaaatcttc tccgctggct
    38281 aaacgacttg tccatctacg atatctccct atgccttcgt tcattccaaa tccatcaaat
    38341 tcaactatta acgtgtcttc tatcacatgt aattcttcat tcaattcata ctcaagtcta
    38401 aaaggaaatt ctccatatgt aatctctggc aatggtggtg ctggagttct ccaaataaat
    38461 acaataactg tgaatagaaa aaatccaacg atcgatataa atgttatgag acctgctcga
    38521 agtaaaaccc gatataataa aaatgctgta tttcgctttg ctatatctag aaaaataaaa
    38581 agtactgcaa tcataatgat agtaaaagtt gtaaaaaaac gcatttttgc cctcccttta
    38641 taaacatcga ttctcaaatt ctaaaccaaa tcttggtagt tcaagttcag gtataacgta
    38701 ttccaaccat acatctcaat tactatttat gaacttatga ttatcactga ataaatcact
    38761 ccatcccctt gttaaaactt ctacattaat tacacctggt tgttttcgac tagctaatcc
    38821 aatgaactta tcaaccatcc ttatccacca accacgagaa tcttgaccag aagtaagaag
    38881 agcagttctc aaggatgatc gagcagttcc ttccagtttt ttaattaatc aggccagtta
    38941 tagataaata cttgatctta tctatatcag gacttctata ttaagcaaaa atttatgtac
    39001 aaaactaaaa tcttcattca gttgtctccc cgctttttat acgattgtat taaaaacaat
    39061 agaagccttt ccaaattatg ttactccagt aaagatggaa ttatagtttg acggaagaaa
    39121 agcaagtata acttttaaaa aaaactgata cattcgcatc tcctgcttca tgtatcagtt
    39181 ttttctttgg attaaactgc ttcttcacgc agtttttccg caaattcatt tattttgcga
    39241 attcggtgat taattcccga ttttgatatc gcacctgagg gaatcatttc ccctaattct
    39301 ttcaagctga cctctggatg ttgaagccga agttctgcga tttcctgaag tttttcagga
    39361 agggcatgta gaccgacctt tgcttcgatc aattcgatgt tttcgatttg tttagaagcc
    39421 gcatcaatcg tcttgttcag atttgctgtc tcacaattga ctagacgatt gaccgaattt
    39481 ctcatatccc gtacgattcg aacatcttca aatcggagca ttgagttggt tgcaccaatc
    39541 aatgttaaga aatcagcaat tttttctgct ccttttaaat aagaaatata gccattgcgc
    39601 cgttcaagcg ttcgagcatg tagaccgtaa taatttaaca tatcgcaaat gtcttggtta
    39661 tgctgttcat aaatagaaaa tatttccaaa tgataacggc tcgtttccgg attattgact
    39721 gaacctgagg ccataaaagc accacgtaag taagaacgca ttttttgcga atttcccata
    39781 atctcgtccg aaacgtgtcc attgaacatc ataccatcca tgatatcaag atccatcaac
    39841 acatcttttg tgccttgctt caaccggacg atatagacat tgttcttttt taatttcatt
    39901 tttttacgaa cgagtagctc acttcgtacc tgataatgat ctttcaaaag agaatagata
    39961 cgtctggcaa ttgcagcatt ttctgtttgg acgttcaaaa taaactgctg gttcacaata
    40021 cctaaagagc cgttcattcg cagcaaagca gctaattccg ctttcgcatg ttctcgatga
    40081 acttccaaac cagttagttc tttcttcaca tctgcagcaa atgacatcct attctcctcc
    40141 tttctccaac aataaattgc ttaatatttt gaaccgaaaa ctaaacggaa caattcttct
    40201 actactttgt ctccatcgtg gaagacacca ccatctttca attccaaaaa gtcggtggaa
    40261 atcactcgac atccttcatt tctcaatccg ttgaagtcgt gtgcgacttg tactagatac
    40321 tcatcataga tctctgggtc catataatct tcaggcactt tttccgtatt gaccaatact
    40381 gtatcgataa aaggctgatt caaatgcttg tgtaaaacac gtatatgatc ggcatctgtg
    40441 aaatgttccg tttccccttt ttgggtcatg atattacaga tatagacggt ttcagcagtt
    40501 gtcttgagca ttgcttcgcc gatttctggt atcactaaat ttggaaggat actggtgaat
    40561 aaacttccgg gtcccaaaac gaccatgtcg gcgtccataa ttgctgaaac gacttttcga
    40621 gccgctttgg cttcactttt atcatttgtg ttgcagacat acacacggtc aatggtttta
    40681 cggtcgatcg cgattttaga ttctcctact gcagtcgttc cgtctttaaa gacagcatgc
    40741 aaggtcaaag gagtttctga agaaggataa acatgtccat ctacatgcat catttttgct
    40801 aataattgaa tcgcctcata agtgcttccc cgcatctcgg aaactgcggc aatgatcaga
    40861 ttaccgatcg tatgattggc taaaaactga tcttcctttt tgaaacggta ttggaaaata
    40921 tcttcataga attgtggcat atccgataac gccactaaga catttctcaa gtcaccagga
    40981 ggagccatag cgatcgattc tctgattgca ccgctgcttc ccccatcatc tgcaacagtc
    41041 actactgctg tgatatctgc tccttgattt cgcaaacttt tcaggataac tggcagacca
    41101 gtgccaccgc cgaccactac gatctttggt tttcttatcc ggtaggtttt cattttcacg
    41161 accggttcac cgtctctttc cttttatcct tgtcccgatg tgtcacattg actttatagt
    41221 ttttccctaa agcatttgcc aaacgttcag tcaatgccac agaacggtgt tgaccgcctg
    41281 tacagccgat tgcaactgtc aagctgcttt ttccttcttt aatgtatcca ggcaatacag
    41341 tttccagaag atccaaaaat ttatggtaga atgattgcgt ttcatcaaag gacatcacat
    41401 aatcatagac tggctgatct tttccggtca atggtcttag ttccgggata taatgagggt
    41461 tgggcaaaaa acgcacatcc atcacaatat ccgaatcgat cggcaacccg tatttaaaac
    41521 caaatgatac aaattcgatc cgaaatcctt ggtctccctt tgatttgaat gactcattga
    41581 ttttttcacg taattgtcgt ggtgtcaatg tcgtcgtatc aatgatcacg gaagcttggg
    41641 ctttcaagtc gtctagtatc gcccgttctt tacgtatccc ttcagtgatc aaaccgtcca
    41701 tcgccattgg atgtgctcgt cttgtttctt tgtagcgagc cactagttct tcgtctgaag
    41761 catctaaaaa taagatagaa gtgtcgatga agttcgtatt ttcgatttcg atcagcatgt
    41821 tttggatttc ttcaaagaag gaacgagaac gtaaatccac tcctaaagcc atttttgtga
    41881 ctttgcctga ttctttgatt aattcccaga attttggaat caatcgaggc ggcatattat
    41941 cgatacaaaa atagcccata tcttcaaaac tttggatagc tactgttttc cctgcaccgc
    42001 tcatacctgt tatgatgact aattgcaaat tatctgtcat aattacgaac tcctctcaca
    42061 ttgtgcatag taaaattata tcattccggg cgtatcatta ccagttttct ttttctttta
    42121 caaaaagatt tggaccaaat atcttttagg atatttgccc caaatccaat ttttttactt
    42181 ttccgatgct gtatccaatg ccttgcttct ttcagctagc gctcgttcaa tcgtccgatc
    42241 tgctaaaata gattgggcat cgatcactgg ataatggtta tcttcagcaa attcatgcat
    42301 caatgacaat tctgtgcaac ctaagataac tttttcacag tttaggcgct ctaccgcttc
    42361 ttctaagatt tcgtaatata gctcttgatt caaataatcc gattccttga tctcatgata
    42421 gatcagataa ttgatttttt cttgaagaac agtatcagga atcactgttt cgaatccaag
    42481 atttttgact tcgcgttcat agattccagc tttcatactt ccttctgttc ctagaatcgc
    42541 tactctccct gtcgtatgct gacggacaag ttcatttgct gcttctcttg gcatatgaag
    42601 gataggaata tccgttgccg cttgtaattc ttcaaaaaag tagtgtgcgg tgttacaagt
    42661 caacacaatg aagttcggct gcaataaatt ttgtttctca atatcatcca gcagaaacgg
    42721 cattggattt tcttctgatc gatctaaaat ataagctgta cgatcaggaa ctgttgcatg
    42781 attgaataag acataattca aatattcttg atcttttgta gctttcgttc ggtggttgat
    42841 caagcgaaca aaactttctg tcgccatcgt gcccattccg cctaaaatac tgaaaaaatt
    42901 ctccatgaac tagccttctt tcacatgaaa atactttttg aatcttggaa tatagttatg
    42961 gaacatatat ttcatcaaca accagcgctt gattgaccgg tctttttcat aaaacacagt
    43021 agtaccgtaa cgattttctt tgatcatttg ctcagctagt tttttgtctt cattttcccg
    43081 agcatattct aaaaagattt ttttgggcac acccatccat aatcttgctt tatctgattg
    43141 attcgttcca taggtcgttt caacaaaggg tttgttaaac actcgatctt ctgtgacgaa
    43201 acgagccaga ttcaagccgt tcaatgtcac aaagaaactg ctacgacctt gtctcaaatt
    43261 gatttcaaat aatttatatt ctccatctct aggatcatac ttcatatcga aattggcaaa
    43321 gcctgtatat tcgatttttt ccaaaaatgc cttaatggtt tgatagattt tttcgttata
    43381 atctggcatg atcacaacgt aattaccaat cgatgctggt gtaggatctt ctaacagtgg
    43441 atgaccaaga cacatcatcc gaacttgatg atcttcatct acatacgcat ttagcacacg
    43501 catattacta tcatcacccg ggataaaatc ttgtacaatc atttcacttt tataaccggc
    43561 ttcgtatatc cggcctaaaa ttaaatcgaa ctcttctcta gtctctaaaa taaaagcttt
    43621 ttttcttcct tcaaattgga cagaaagata ctccacactg tttgcaggtt tcaaagcaac
    43681 tgggaattca aaaggcaatt cctgttccaa atgtccattt actaacattt cttctctgac
    43741 gatcaatgtt ttaggatacg gaagatcata ttcttcacaa acttcataaa agcttacttt
    43801 attgatcagt ctttcaaata aagaataatc aatatatgga cagataaaat attctgacag
    43861 ctcttgttta tgttgggaga tcaattctgc atatccatca ccacaagcaa tcaaaagata
    43921 ttttttactc ttatcagaat atctttcttt tgccaaacga agcatcgtct cgataaacac
    43981 agggtcttta tcaaatcctg gaataacttc tacattgaca attttactgt agcgggtagg
    44041 ggctaactga tccgaagcat atgcttggca gattttgccg tatgcttcat taaaagaacg
    44101 tgccatacca taaacgttca tgtcgcttcc taataatata gggataaatt cttcattttc
    44161 aatactgttc atcattatct ctcacttact cataatctct tgtttttctt ttccaactac
    44221 acaaggaata tcataccata aagaaaaaag caatcaaagg aaactcctcc gattgctcaa
    44281 ttcttttttg aacggattga acttatagga agtttttgtc tataccaatc agtgtagaaa
    44341 gaactaccat gtaatgattc tagaacactg atattaataa aacaagcaaa aattcatttt
    44401 tttatgtttt tttaactatc tcagctatct atcctcttta taccattatt tttttcaaat
    44461 agtgacctgt ataactttct tttacgttaa tgatttcttc tggtgttcca gtcgcaagga
    44521 tcgttcctcc accttctcca ccttctggtc ctaaatcaat gatataatct gctgttttga
    44581 ttacatctag attgtgttca atcactaaaa ctgtgttacc agcatctact aatctttgta
    44641 atacatgcaa caagcgggcg atgtcatcgc tatgaagtcc tgtcgttggt tcatctagta
    44701 tatagaaatt ctttccatta gagattttgt gcaattcact ggcaagtttc atccgttgtg
    44761 cctcaccacc ggacaatgtc gttgctggtt gccccatcgt cacataacct aagccaacat
    44821 caacaatcgt ttgcagtttg cgatgaatct ttggaatgtg cttgaagaat tctacagcat
    44881 cttctactgt catttccaaa atatcagcaa tgctttttcc tttgtaatgc acttctaatg
    44941 tttcagagtt atatcgtttg ccatgacata cttcacaagg aacatagaca tcaggcaaaa
    45001 agtgcatttc gatcttaata attccatccc cgcgacaagc ttcacaacga ccgcctttta
    45061 cgttgaaact aaaacgccct ttcttataac cccgcatttt tgcctcgttc gtttgagcaa
    45121 ataacccacg gatatcatca aatacacttg tataagtcgc tggattactt ctcggcgtcc
    45181 ggccgattgg gctttgatcg atatcgatga tcttttcgat actttcgtag ccggaaattg
    45241 tcttgaattt acctggctta gcagaattct tatttaattt ttgcgctaac gatttcttta
    45301 agatactatt gaccaatgta ctctttccag aaccagacac acctgtgact gctacaaatt
    45361 ctcctaatgg aaattcaaca ctaatatttt tcagattgtt ttctgaagca ccagtgatct
    45421 tgattgcttt cccattacct ttacggcgtt cttttggtac agggattgat tttttccctg
    45481 aaagatattt ccctgtcaaa gaattttcat cattagctac ttcttcagga gtgcctgcag
    45541 ccacgatttg tccgccttgt tctccggccc caggtccgac gtcaatcaga taatctgctg
    45601 cccacatcgt atcttcatca tgttcgacta caacgagggt gttgccaaga tcacgcattt
    45661 tctttaatga gccaatcagt cgatcgttgt cgcgttgatg caatccgatc gatggttcat
    45721 ctaaaatata caaaactcca gaaagattcg atccaatttg tgtagccaat ctgattcgct
    45781 gcgcttctcc cccagataat gttccggaag cgcgactcaa agttagatag tctagtccca
    45841 cattttccaa gaatgaaagt ctgtcattta cttcttttaa aatcggtttg gcaattactt
    45901 tctcttgttc agataacgtt aagtttgaga agaaatctac tgctttttga atagacaact
    45961 catttgtttg cccgatattc gttccattga tttgaacaga tagagcttgg ggattcaatc
    46021 gataaccttt acatgtctga caagttaatt ctgtcatata aagacgcatt tgctcacgcg
    46081 tgaaatcact gttcgtatca tgataacgac gctcgatatt cttcaatacc ccttcgaaag
    46141 tcgtttctac atcgcgaacg ccaccaaaat cattttcata atggaaatgg aaaagatcat
    46201 ctgtcccatt caagatgata tcctgatgct cttccggtaa ttcttcgaat ggtgtatcta
    46261 aatcgatacc gaaactttca caagcctgcg ctaacatttg aggataatac tgagagctga
    46321 ttggattcca tggaacgata gcaccttgac taagtgtctt cgttctatct ggaatcacta
    46381 aatcttgatc gacttctaat ttgacaccta aaccatcaca gtctggacaa gctccaaaag
    46441 gcgcattgaa tgagaacagt cttggttcta gttcgccaac agtaaatccg caataaggac
    46501 aggcatagtg ttcgctaaac aacatttctt cttcaccgat cacgtcaacc agagcatagc
    46561 cgtctgccaa tcgcagagcc gcctctaaag aatcaaatag acgagaacga atcccttctt
    46621 tcacaatgat ccggtcgatg acgatcgcga tatcatgttt tttgtttttc tccagctcag
    46681 gcacttcact taaatcatag atctcaccat cgactcgaac gcggacgtat ccctcttttt
    46741 ggattcgttc gaatattttt ttatgttgtc cttttttctt taccacgatc ggtgccaaca
    46801 cttggatctt tgtgcgctca ggcaaagcca ttacttgatc tgccatttga tctactgatt
    46861 gactggtaat ctcgatatga tcgttgggac aaatcggatg tcctacacgt gcaaacaaca
    46921 agcgtaaata gtcattgatt tccgtcacag tacctactgt cgaccgagga tttttacttg
    46981 ttgttttctg atcaatagaa atcgccggac tcaatccgtc gatgctatct acgtccggct
    47041 tatccatttg tcctaaaaac tgacgagcat aagcagaaag gctctctacg tatctccgct
    47101 gcccttcagc gtacagcgta tcaaatgcta gggaactttt tccagaaccg gacaaacctg
    47161 taacgaccac gaatttatct cgtggaatcg ttacatctat atttttcaag ttatgggcac
    47221 gtgcaccgtg aatcgtaatt ttatcgttag ccatgttgtc ctcctacttt gaagctttta
    47281 attctaaaat catatctcgc acattagcgg ctgtttcaaa atccaatgct ttcgccgcat
    47341 ttttcatttc tttttctaat ttcatcaata acgtatcttt ttcttctttc gtcatttctt
    47401 catacgagac ttgtaccgct tcttttgtat catcttccga ttctttcgta atagaaatca
    47461 aatctcggat ttctttaatg atcgtttttg gaacgatccc atgttcttca ttgtacttct
    47521 gctgaatcgt acggcgtctt gatgtttcat ccatcgctag acgcatagaa tctgttactt
    47581 tgtctgcgta catgattact ttaccttctt cattccgagc agctcgtcca atcgtttgga
    47641 ctaaggaacg ttcactacgt aaaaagcctt ctttatccgc atccaagata gcaacaaggg
    47701 aaacttcagg tacgtctaat ccttcacgaa gcaaattgat tccaactaac acatcaaatt
    47761 cacctaatcg aagatctcga atgatttctg ttcgttccag cgttttgata tcactatgca
    47821 aatatttgac tttgatccct aattctttga aataatctgt gaggtcttct gccatttttt
    47881 tcgtcaatgt cgttacaaac acccgctggt ctttttcgac tcgttcattt atttcgccta
    47941 ctagatcatc gatttgtccc atgattggac gaacttcgat cactgggtca agcagccctg
    48001 tcggacggat gatttgttgg ataacggtat ctgtttgttc atgttcatat ggtcctggcg
    48061 tcgcagagac ataaatgatc tgattgacat gtttttcaaa ctcttctaac cgaagtggcc
    48121 gattgtctaa agcagatggc aaacggaaac cgtaatctac tagcatctgt tttctcgctc
    48181 ggtcaccatt atacattcct ctgacttgag gcatggttac gtgcgattcg tccgccacga
    48241 tcaaaaaatc ttcaggaaag aaatccaata gcgtataagg cggttcacct tctttacgtc
    48301 cgtccatatg ccgagaataa ttttcaattc ccgacgtgta gcccatttct aacatcattt
    48361 caatatcgta attggttcgc tgttctaaac gttgagcttc cagaagtttg ttttcatttc
    48421 gcaagacagt taaacgctgt tctaattccg ctttgatatt cgctacggca tgctccatat
    48481 gttcatcatt cgtcacaaag tgagtcgccg ggaagattgc cacgtgttcc gtttctccta
    48541 aaacttcacc tgtcagtgca ttgacttctc ggatacgttc gacctcatct ccaaaaaact
    48601 ccacacgtaa ggcacgttca tctcttgaag ccggaaaaat ctccacgaca tcaccgcgca
    48661 ctcgaaaacg accgcgctga aaatctatat catttctttc gaattggata ctgactaagt
    48721 cacgaatcaa ctgattgcga tctagttctg ctccctgtcg aatcgaaact acttgttttt
    48781 ggtactcaaa aggcgaacct aatccaaaaa tacatgaaac ggatgcaatg acgatcacat
    48841 catttctttc taataatgaa ctagtagccg aatgtcgtaa tttgtcgatc tcatcattta
    48901 cacttgaatc tttttcgata tacgtatcgc ttgaaggcac ataagcttca ggctgataat
    48961 aatcataata agaaacaaaa tattcaacag cattgttagg gaaaaattcc ttgaattccc
    49021 cgtacagctg accagccaat gttttgttat gggcaataat tagtgttggt ttattcactt
    49081 tctcaataag attggaaatc gtatatgttt tccctgttcc agtcgcacct aataagattt
    49141 gtgctttttt cccgccgact acgccatcca ctaattggtt aatcgcttca ggctgatcac
    49201 cagccggttg gtatttcgag acaagctcaa agtgtcggga ggtatctctc tcaatcattc
    49261 ttcgtcctcc ttttcattca ttccttttca ttttacgacc aataaaaaga aaaggaaagc
    49321 aaaggcattc cttactaaaa aattatacca caccgaacat actttcgcca cttttttatc
    49381 ttattatgca tgttacgttt tataacataa aatgttagaa actcgcattt ttttcatttt
    49441 ttcgagctta cagtcttgtc tgattcagca cttttcgcct ataataataa aatattggaa
    49501 atttaggagg ttgattatga aaagaaagca tttatttcac tcatttgttt tcttaatgac
    49561 aggacttgtg acacttgcgt ttggacatgt tgcacaagct gacaacacta caacggcaga
    49621 gtcaagcaca agcgagacaa tgcaaaaaat agagcccaaa aaagacgtat acgtaattgc
    49681 cagtgactct gcttttgctc catttgaatt tcaaaacgca aagggcgagt acgaaggcat
    49741 cgatgtaaaa ttgatggaaa acatcgccaa actgcaagga tttaacatcc gaatggacta
    49801 ccgtggtttc tctgctgctt tgcaagcttt ggaatcagga caagcagacg gaatgattgc
    49861 cggaatgact gtaacagatg aacgaaagaa aagctatgat ttttctgatt cttactttga
    49921 tagcgggatc caattagctg tcaaaaaagg agatacttcg atcaagtctt atgaagattt
    49981 gaaaggaaaa acagtcggag caaaagttgg taccgaaagt gctgaatttt taagcgacca
    50041 tgaatctgaa tatggctatt ctatcaaata ttttgatgct gctgatcaat tgtacgacgg
    50101 gttaaaagtt ggcgcaatcg atgcaatgat ggatgattat ccggtaatcg gttatgcgat
    50161 caaacaagga caagaattgg aaacaccgat caaaaaagaa tctggcggaa aatatgcatt
    50221 tgccgttaaa aaaggacaaa atcctgagct tcttaatatg ttcaatgaag gattgaaaga
    50281 attaaaaaga acaggcgaat acgataagat tattaaagaa tatgtagcgg atggttcaga
    50341 aacttccagt gagtcttcca ctgctgatga atcaacattt cttggactta tccaaaacaa
    50401 ctatcaagca ttgttgaaag gactttggag aacaattctc ctaactttga tctcctttgc
    50461 tttggctata gtaatcggtg tgatttttgg attattcagt gtagcaccaa tacgcgctct
    50521 tcgcacaatt gctagtattt atgtcgatat catccgagga attccaatga tggttcttgc
    50581 gttctttatt ttctttgggt tgcctggtat cgttggattt acgattcctg acttccttgc
    50641 tggtgttatc accttgactc ttaacgcaag tgcttatatc gcagagattg ttcgaggcgg
    50701 gatcaacgcc gttcctgtcg gacaaatgga agcttccaga agtttaggtc tttcttataa
    50761 tcggacaatg caaaaaatca ttttgccgca agcgatacgc atcatgatcc catcattcgt
    50821 caatcagttc gttatctcat taaaagacac aacgatcatt tccgcaatcg gtgtagtcga
    50881 attgctgcaa actggtaaga tcattgttgc acgtacaaca caaagtacgt atgtttactt
    50941 gatcattgcg atcatgtact tgattttgat cacagcattg acaaaattag caaaagtttt
    51001 agaaaagaag gtgaagtaag atggcagaaa aaatcttagt tgagcattta gtcaaaaaat
    51061 acggcgataa cacagtatta aacgacatca atgtgtccat caacgaagga gatgtcgtct
    51121 gtatcattgg cccttcaggt tcaggaaaaa gtacattttt gcgttgcttg aatcgtttag
    51181 aagaaccttc tgacggtgaa attattattg acggcgccca cttgatggat aaaagcacag
    51241 atatcaactt ggtgagacaa catatcggaa tggtattcca gcactttaac ctattccctc
    51301 atctttctat tttggaaaac atcattttag caccgatgga cctgaagaaa gaatctagag
    51361 aagaagcaga gaaaaaagca atcaaattgc tgcagacagt tggtctagaa gataaaaaag
    51421 atatgtatcc tgaaatgctt tccggtggac aaaagcagcg tgtggctatt gctcgtgctc
    51481 tagcgatgaa ccctgacatc atgctttttg atgagcctac atctgcactc gaccccgaaa
    51541 tggtcggtga tgtcttgaac gtcatgaaaa agttagcaga acaaggtatg acgatggtta
    51601 tcgttacgca cgaaatgggc ttcgctaaag aagtggccaa tcgtgtcatg ttcattgacg
    51661 acggaaactt tttagaagac ggaacgcctc aacaaatctt tgaaaatccg caaaatgaac
    51721 ggacaaaaga tttcttgaat aaagtattaa atatttaata aaaaccaaaa atgcccggtc
    51781 gcttcatgac gaccgggcat ttttagtttc ttgttttttc acttaactgt accagccgca
    51841 tcgcttgttc tcctgcacaa acaagcaatc gctctacctg ttccatcgca taagaaagta
    51901 ccatcggttc attgacgata tcaagtacat agtcgatacc accttcagaa actatgatga
    51961 ttatcttatt ctaaagctgg cttttttgtt ggtacaaaca tgacaactac aaaaaaacca
    52021 ggcgaacgta tgattgttcg tctggttttt aattattaat gacttagcgt ttttactaga
    52081 aatagaaaat ttgggcaagt ctactttata tttttactag aaaaactact tccgcttttt
    52141 cccgccaaaa gcatctttaa acttatcgaa gaaaccgtct tcttgctgtt cttcaacttt
    52201 gatgccgcca gcttcagcga atgcacgtaa tgcttctctt tgttgttcat ttagattttt
    52261 cggtgtgatc agttttactt tgacatgttg gtcgcctgtt gcaccaccac gtaacttcgg
    52321 agcgcctttt ccacgtaaac ggaagttcgt acctgtctgt gttccagcag ggatcttcaa
    52381 tttcacatct ccatggacag ttggtacttg gacttcatcc cccaaagctg cttgtacaaa
    52441 acttaaaggc aattcatagt aaatttcaga accttcacga tcaaagatat cactttcttc
    52501 tacacgaaaa accacataca aatcaccaaa tggtccgccg ttttctccag cttctccttg
    52561 accagctaaa cgcatttgtt gtccttcttc tacaccagct ggtacattca ctttaactga
    52621 atgtgctttc ttctcatgtc ctgagccatg acaggttgga caaggttctt tgatttcttt
    52681 ccctgtgcca tgacaaacat cacaagtttg acggctcatc actcgtccaa gtggcgtttg
    52741 gcgttctaca ttgatcgtac ctgcaccatg acatttatga caagtagttg gttgtgtgcc
    52801 tggttttgca ccgtttccac cacaagtatg acagatttct tcccgattat acttgatttc
    52861 tttttctaca ccaaaaatcg cttcttcaaa agacaagtcg accgtatatt gcaaatcagc
    52921 accttgacga ggcgcatttg gatcgactga ccgaccgcct cctccaaaga aagagtcgaa
    52981 gatatcttca aaaccaccga agccgccacc agagaagccg ccgaatccac caaatccgcc
    53041 accagctcca ccagcaccgt aatttggatc tgtgcctgca tgcccgtatt ggtcgtatgc
    53101 agcgcgtttt tgcgggtcac ttaaaatctc atatgcttct gatacttctt tgaatttttc
    53161 ttccgcatca ggttctttat tgatgtcagg atggtatttt ttcgaaagct tacgataagc
    53221 tttctttatt tcatcttctg atgctccttt tgagagccct aagacttcat aataatcacg
    53281 ttttgttgcc ataggctttc ctcctattgg ttctcatcat tttcacgtcg cttgttatca
    53341 tatcatattt tgtttgaaac aaaaaccgat acttcaaact atttttcaaa agaaaaagcc
    53401 aaagtagcaa ctttggcctt ctctcgaaat tcagtgaact gattatttat cgtcgccatc
    53461 gacttcttca aaatcagcat caacgacatc atctgctccg ccttgagctg cttctggatt
    53521 ttcttgagct tgttgttgtg cagcttgttc gtatagttta acagtcaagt tttgaacgat
    53581 ttcgttcaat gcatcgcgtt tagttttcat ttcctcgatg ttgtttgctt caacagcggc
    53641 ttttaattcg tcacgagctg tttctgcttt tttcacttct tcttcgtcca ctttgccttc
    53701 taattctttc aatgttttgt ccacagagaa tagcaatgcg tctacatcat tacgtaggtc
    53761 aacttcctct ttacgagctt tatctgcttc agcgtttgct tctgcatctt tcaccatacg
    53821 ttcgatttct tcgtctgtta gacctgaaga agatttgatc gtgatttttt gttctttttg
    53881 tgtacctaga tctttcgcag atacatttac aatcccgttt ttatcaatat cgaatgttac
    53941 ttcgatttgt ggaataccac gaggagctgc tggaatatct gtcaattgga atcttcctag
    54001 agttttgttg tcagcagcca ttgggcgttc accttgtaat acatggatgt ctactgctgg
    54061 ttgattatct gccgcagttg agaatacttg tgatttactt gttggaatcg ttgtattgcg
    54121 gtcaatcaat ttagtgaaca ctccacccat tgtttcgata cctaatgaca atggtgtaac
    54181 gtcaagtaaa acaacatctt tcacatcacc agtgatcacg ccaccttgga tagcagcacc
    54241 catagcaact acttcatcag ggttaactga tttgtttggt tcttttcctg tttctttacg
    54301 tactgcttct acaactgcag ggatacgagt agatccaccg actaaaataa cttcgtcgat
    54361 ttctgattgt gacagacctg catcttttaa cgcttgacgt actggtactt ttgtacgttc
    54421 tactaagtca gctgttaatt catcaaattt tgcacgtgtc aatgtcattt ccaaatgcaa
    54481 tggtccagat tctcctgctg tgataaatgg caagctgatt tgtgtgcttg atacgccaga
    54541 taaatctttt ttcgcttttt cagcagcgtc tttcaagcgt tgtaaagcca ttttgtcttg
    54601 agacaagtca atgctgtttt cttttttgaa ttctgctacc atgtagtcga tgattttgtt
    54661 atcaaagtcg tccccaccta gatggttgtc gccagctgtt gacaatacat caaagacacc
    54721 gtctcctagt tcaaggatag atacgtcaaa agtaccgcca ccaaggtcga atactaagat
    54781 tttttcgtcg cgatctgttt tatctaaacc ataggcaaga gcagctgcag ttggttcatt
    54841 gacgatacgt tctacttcta gaccagcgat cttaccagca tcttttgttg cttgacgttg
    54901 tgcatcatta aagtaagctg gcacagtgat aactgctttt tcgacttttt cacctaaata
    54961 gtcttctgcg aatcctttga tatattgaag gatcattgct gagatttctt gtggtgtata
    55021 tgatttacct tctacatcta ctttgtaacc tgcttcaccg atatggcgtt tgattgatga
    55081 aatcgtattt gggtttgtca cagcttgacg ttttgcaact tcccctactt ggatttctcc
    55141 atttttgaat gatacaacag aaggtgttgt acggtttcct tctgggttag cgatgatttt
    55201 tgcttcgttt ccttccaata ctgctactgc agagttagtc gtacctaagt caataccgat
    55261 aattttactc ataattaacg tctccttctt taattgaaag tgtaagtttt atatccgcac
    55321 aattattgtg caacaatgac catggttggt cttaacacgc gatcatgtaa tttgtagcct
    55381 tcttgtagta cttctacaat cgtatcggct ggtgtatctt cagtcgctgg aacagtttgt
    55441 actgcttgat gaagattagg atcaaaggct tctcctttag cagggatttt ttcaattcct
    55501 tcttcttcca gtgcgttacg caaactttcc aagaccattt ccacgccttt tttcaaacct
    55561 gccccttgat catctgacac ttctgtggct aatgcacgtt ccaggttgtc aattgatgga
    55621 agtaattttt tggctaaatc ttgtgaacgg tatttttgta attgctcacg ctcatttttt
    55681 cctcgatttg ccatatttgc gatttcagca cgagcccgca agtatttatc ttccatttct
    55741 tctaactcag cttttaattt cgctgtttcg aattcttccg cttcagcttc tgaaataccc
    55801 gaatcttctg cagcttcgac atcgatttcc ggttctggct gagcatctgt catttcttta
    55861 tctagttctt cttgattttc tttcacgata cagtcttcct ttcattattc agtcagctgt
    55921 ttgattgctc ttactaagaa agagagaacg atcagctgca aatagcttcg ttattcacat
    55981 tacgagtttg acaagtcaag cgaacggtag taatcagcaa gtttagctgc tagttctctt
    56041 cgataaacat cgactagacc gaacatccga gaataaggca tactagtagg ccctaacaag
    56101 gctattgtac ctcttccatg accatctatc tcgtaacttg cttgaatcat actcatattc
    56161 tgcaataaat cattgcctaa ttcagcacca atcttgatat gaatattgct atccatcgga
    56221 gtgatcattt gtgtaagtcc gtctgagtct ttcataaaag aaaacatcga tttgaactga
    56281 tcaagatctt gacggggctc ataatccaag agattcattt gtccactgac ataaacctta
    56341 tcttcaaaaa catgaccaag aacggtgtca aataactgag acatcccttc tgtcgtttgg
    56401 aaatatttat gaagaatcat cggaatctcc gtacgaagtt tatggtacac cgtcatcaga
    56461 ggatctccga ctagacggtc gtttacaatc tgtaccattt tttctagttc ctgacttccc
    56521 atatattcgg gaagagtgaa cacttggctt tctacattcc ccatatctgt tacgacgata
    56581 gcaataatct gtcgattatt caagggtacg atacgaaagc cagtcagtcg gcggtctttt
    56641 atttcaggtc ccaatgaaaa agtcgtatag cttgtcagct cagataatat ctcagcagat
    56701 tgcttaataa tctcattgat ttcatgaaat tctctgccta gagactgacg aatcagcagc
    56761 aaatcatctt ctgctacgcg agcaggtttt aacaaatgat ccacgtaata acgatagcct
    56821 aaagcagatg gaatccgccc agaagaagag tgagtcttca acagcaatcc ttcatcttct
    56881 aatgctttca tatcattgcg aatcgttgct gagctggcct tgactccctc agacatcaat
    56941 gttttggaac caacgggcac gccactactc gtatagtgtt ggatgatcag acgtaaaatg
    57001 tcgttttgtc gctttgttaa catcttcctc acctctcatt agcactcata tattctaagt
    57061 gctaatccac ttataaatat agcaagaagc ccttctcctg tcaagagaaa gactccattt
    57121 tttagcactc taaacacaag agtgctaatt ctaaataatt gatgtaaatc cagttatatc
    57181 aacgtttcaa tagtccttta ttgtattttt gaactttttt tatttaaatt cagataaaaa
    57241 acaaatattc tttaaactat ttttcaaaca aaaagcgctc aaacacattg ttccccaaaa
    57301 aggtccctct tggtgtcaaa taaacacggt cttcttcatt aacaagtaac ccttcttgct
    57361 ctaattccct tagcacatca ccgtacaccg attcaattgt tttttgatag cgatcataga
    57421 aatgtttttt agtaatccct gttttttttc ttagtcctaa gaacatttct tcttctattt
    57481 gattttttcg tgtcaatttt tcttcttcca ataccgggcg ttcatttgct cgtaatgggc
    57541 gtaaatagtg ttggattggt cctttgttgc ggtaacgaat atctccaaga taaccgctag
    57601 caccagctcc aaacccaaaa tagtgggcat tgttccagta catgaggtta tgttgagatt
    57661 catgtccgga gagcccaaag ttacttattt catattggtg acgtccatgc ttttccattg
    57721 cttcaatcgt ctcttcaaac attctggttt ccgtttcctg atcaggcagt tcgagtcttc
    57781 cttgtcgtac ccagttcatg aacattgttt tgttttcgag gatcaatgag tacaaagaat
    57841 agtgaggcag atccaaagcc aaagcttttt ccaaagtatc cagatatcct tctagtgttt
    57901 gtcccggtaa tgcataaatc aaatcgatgc ttacattggt aaaattttct gcttctaaaa
    57961 attgcatagt ttgatacaca tcttctgcgg tgtgtttacg tccaattttt ttcaataagc
    58021 gattatcaaa tgtctgcact cccatggata aacgattgac gccatagttt ttcattactt
    58081 gaagtttttc acgggtcaaa tcacctggat tcgcttctac agtaaactca ttaccatttt
    58141 gaaaaggcag gatctctcta gcacctgata acagtcgatc aagttgttta gcagataaag
    58201 aagttggtgt cccgccccct acgtaaagtg tttctaaatt cgctgtcgga tgactttcta
    58261 gcattatttt catttcattc agcagcattt ccacatactc atctactggc tgcccttcta
    58321 aaaaaacttt attaaaatca cagtaatagc agatatgctc gcaaaatggg atatgaatgt
    58381 acgcagaaac cttgttttct gcgggattta ttgtttctat cattgaaatt ctttctcctt
    58441 acgtcgaaaa gtcgtgcctg ttttcgacct tcttaaaatc gcgttcaagc accaactcat
    58501 tgaaataagc cgcatattta gaatcaagga aacctagctc taaaaatgct tgcttattgt
    58561 catcggagct acacggtgct tttacagcct ctcgataaac ggtaatcgca aagctgacct
    58621 tcggcattaa gtcaaaatct gcaaaaatat gaaaagctat tttcacagat tcctccttaa
    58681 gtgctcagac cacaacgctt tgcttacaac ctctcatata cgcatactaa acgtatttgg
    58741 cgaacaaatc cactaaaaaa tccaagaatc gacttcttgg atgatttttt ctttcattaa
    58801 actggttacc tagttatata tctctagggt tgttttcatg tctgagtgcc cgatatgatt
    58861 tatttttcgt ttctataatt actttttgcc ttccagtttc ttttaatatt gtactcaagc
    58921 aagtgggaat tggttctttt gtcatccatg tatgcaaaat gttataatgc tggtaaacaa
    58981 aaatgattac ctgtcatttt tatttagttc gtatttaatt aagttgacta tctgtatact
    59041 ctgtctaaaa cttaagaatt gttgttttcg ctgtcgctac ccaattttaa tgaatgtctg
    59101 taggttaaaa ctgttgtcct tgttatagat agtaaaggag agcttgaaaa tgaaaactgc
    59161 gattacgttt ctattagagg aatatgccga ttgggaaggt gcttatatta ctagcacact
    59221 aaaccaaaat gagaaatggc aaaacaaaac tgcttctatc caatcagagg tcacttccat
    59281 tggaggatta aaaacaaaaa tcgattactt aatcgaacaa atacctgaaa aagttgacct
    59341 ttttattatg atcggtggca attcatggga cataaaaaat catcatttat atcaaataat
    59401 cgataaattg ttaaaaacag acgtaccagt agctgctatt tgtggggcag ttgattattt
    59461 agctcagagc ggattattgc aaggttacaa acacaccgga aatgcgcaat ttccttggtt
    59521 agaatcggaa aattatcaga ataaagaaga ttttatagaa caacaagctg tgaaagatcg
    59581 taatttgatt actgcgaatg gaacagctcc tatagaattc acagacttga ttcttcaagc
    59641 agtccatttt gaaacagaag aaataagaga aaaaacgatt cgtctataca aacttggatt
    59701 ctatgattac tataaaaagt acggagatcc tttctcctaa tcattctctt caattaaata
    59761 ctccttatca atagtgtgac aagtaatttt actgatgatt atttgtcaca ctttttctga
    59821 catttatcaa agaagataca ctctatttat attcatagac aaggttttac ggattcaaaa
    59881 attcaggatt atgctatact ggtattaaaa aaataatgag gtattttata tgaaaaagtt
    59941 aggttttatt ttattgttgg tctctttatt ccttgtagga tgtacgtctc ctagtaaaga
    60001 aaatagtgat ccatctgcga ccacagacaa aagttcaatc agacaaacca gttcagctga
    60061 aaaatcaagt actcagaaat cagcttctac tgaaacgaat acatcttcaa caagcagtaa
    60121 agtctcagag cccaatactt cttctacatc tagtagtaca gttgagacaa atatttcttc
    60181 ttcaacaata gacacaacct cctcacagtc ttctcagtca atagatccgt tagcaggata
    60241 ttctgatcta caaattgagt acgcacgtgt ttggctagct attagaggta atacttttag
    60301 agatacatcg ggtgatttcg aattacatgt tattcattat cctgcaggaa cacctatcgc
    60361 cccttatgat gcagggagtg cagtctatcc gaaagatgtg gttatgctca ctgggaaata
    60421 cagttaccag ggagttttag tgtattcaag taaccatgac gggacaatca ctagttatcc
    60481 agttccttca cattggcaaa tgcctgcaga tcaaacatct tatccagcgt tcattagaga
    60541 aacaactcaa gaaatcattg ataatgcatc tgtagtagct attcctacag gaaatcccaa
    60601 tgacgtgaaa cagttaatcg atgtgacagt tatagataat tagactttct aatatgttat
    60661 acaaaaagaa gctgacagca ttttgtcagc ttctttcact ttcttttcta tttagattta
    60721 ttggtctttg atagactcca aggcaggaaa aaatttttta aataaacgtt tgcaaaacag
    60781 tcgcttttct atcttctacc gctacttctg cataggcacc attaatcaat gtcagttgtg
    60841 gcggtacatg accacaatca atatcataca agatcggtag ttttaattcc tttgacagat
    60901 cttcgtatac atccttcatt ttataatcta acaaatcctc tgccgcactt ctaccgaaca
    60961 tgatccccga gctgccatca aaccagcctg ccagtttcat ctgaacaagt gatcttctta
    61021 aatcaactga cgatagctca cagttttcta gaaaccacaa gatcggcttt ccatcattat
    61081 actttctttt aaaagattgg acatctccat aaggagtacc gattaaatgc ctgatcgtat
    61141 caatacaacc acctaataat cgtcccttca tccttactgc atctccgctg actgattgcc
    61201 aatttgttgg ttcagtgaga tggaaaacag aagatgatga atgctcatgg ggccacactt
    61261 tttgatactt ttcagaagat tgctgtagaa tcgattctcc ttctcttgtt ttcagtacac
    61321 tttcccacat tgctgtcgtt tcatccaagt actctcctct taaatcaata aggttagttc
    61381 catgtgcagt ggctatccct gtttttaatg tgacagcaag tagcaacgta ctaatatctg
    61441 aataacctac tatccatttt ggttgtagag catcaaaatc gacaaaatcg agaatctcaa
    61501 tcaataattc tcctccccaa gtcggaaaga tcatttggat attttcatct tgcatcattt
    61561 gattgaattc atctgctcgt tttcttgctg gacctgattt ttccttatct tgcgtccaaa
    61621 gcgtctttcc agtaactact tggaaagatt tttcttccat tctctttaaa gctgattcga
    61681 gtaacgaatg gtgttcctct gatacgcctg aggatggtgc agtaacccca atcgttgcat
    61741 ttctttctaa taatggataa cttatcatgt tgcttcacct ccaagtatgc actattttac
    61801 ttggttaatt gtttttttcg tttgccggca tcaaaaatat cggcatctca agtagctgga
    61861 tgattttgtt taagtcaacc gtttggatga ccatcgtttg atctggtcta cctggtgcaa
    61921 aaacaagttt ttcatttgat aagagctctt catcaataat aatcggaatc tgtttttcat
    61981 aaccaaacgg agaaacagcg ccctgctttt gtcccgtttg ttcttccatg cgttctgatg
    62041 aaacaataga aagcctttta ccgacgatct tccctaatgc tttgaaatcc gtttgtttcg
    62101 tagtaaaagt cagatatacg tagttattat cttttttatc ttttaagaat aagcttttgg
    62161 tctctgtacc tgtaaatcct tgttctttct ttattaccaa agcatcttca tttgtataaa
    62221 tggctcgatg atgatacaac tcaaaatata tattttcgtg taagaatttc tttgccaagt
    62281 caatcatttt tatgtctctt ttctatttta ttatttgaga catcttatca tataaatata
    62341 gcggttacaa cattacttgt gttttgttcg ttttttatac ataactattg tttttctggt
    62401 aaaataaatc aaaccactta atggaggcgt tttagaatgg aaaaatttca gccgatcatc
    62461 aatcaaatca aagaaaaaga acatcaacaa aagatcaaag acctttttgc taagataaca
    62521 gaaacgtatc cccagctaga cacaactgta aaatggaacc aacctatgtt tactgaccat
    62581 ggtaccttta ttattgcatt tagttttgct aaaaatcatt tttctatcgc accggaaaaa
    62641 gcagcgattc gtgcccttga aaaaaatatc caagaagcag gctacgtata tacggataac
    62701 gtcatcaaag ttccttggaa aagtgtaatc aactgggaat tgattgaaca attgatttca
    62761 tttaatattg aagataaaaa aggtcatgat aaattttggc gataaagctt tatacgaata
    62821 aaccttttac tggcgaggaa actattcatc accttataca ggttttgttc aatcctatcc
    62881 tttttatcat ccctggttaa acacttaatt gacaaagtat ctgctatttt tataaggagt
    62941 ctttcttgtg ctgaaaaaaa tcactgattg aatggctttg aattttctac ttagtaaaat
    63001 tattttttca gcaattttcc tgcaaaactt ttgaataagg aaaaaatacg agaaaatatc
    63061 agtgatcttt cataatactg cccattattt tcaggtatat ttaaatataa tatcacttta
    63121 ggagagaacg aatttcttta gagaaataaa agttagaatt ttctgacttg atttttacaa
    63181 tgaccttaat taaaatcgtt ctttctacat ttcgtgatta agcaaatatc aactaagtca
    63241 gtagaaccat tcacatagga gggtgaacat tgaaagattt ttactcagta aatgaattag
    63301 ccgaacaatt aggtgttact acacgttcta tcagaaatta tcttcatgaa ggaaaactaa
    63361 aaggaacaaa ggttggcggg caatggaaat tttctgaacg taatcttttt gagttcctat
    63421 acggagatca agcagaggaa gcagcaaaag aaatgcagcg attcatgcta aatgcaccaa
    63481 tcacgatgcg tttcaacctt caatatcgtg attttacagc aatcaatcaa ttccgagaac
    63541 aattagtcca gtaccataac gatgtttacg caaataaaaa agaccgtctt ttacaatacg
    63601 atctttataa agacaatcat gcggagatac taatcggtgg taacttcaat tatgtcgtaa
    63661 actttagtca atggatcaat gaaaaattat tgatccagac agatatttcc cttgtatctt
    63721 aaacaattaa aaagcgctag aaattccaga gatgttggaa attctagcgc tattttttta
    63781 taagtactta ctgtttggtt caaacaaata tttttttaat aaatgaaatt ttaccatctt
    63841 tatcctcaaa taaagaccgt gaccatgctt cctactacga tcagcaataa tccgataatc
    63901 gttaattttt tttcttttgg tgtcttattt tcatgcagga tgtacatacc gcctaatgta
    63961 gagataacta cgtttagctg tgtaatgata tatgcagtag caaccccatt ttcatttgct
    64021 gaaaaaatat aagccaatgc tgacacacta aagatggctc caacgatcgt tgctttccaa
    64081 ctcttgtctt cagaaaatgc ggttggttta cggaaaatca cataaatcac tgcccctaaa
    64141 aagacaccga tcatttgcgg aaagaaaatg gaaagtccgc ttgcatcaac tagtttaggc
    64201 aatgcactgt atacccaata accaatactt gttgatgcta atagactaat tccttttgtg
    64261 ataccgcctt tttttccagg atcatcactg attgccgtta aagcagaacc aaatacaaga
    64321 agaatgattg ctgctgctcc aatgattttc cccatacttc cagaccattc tccaaaagcg
    64381 aagacaccaa tcaacgaggt tccgactaac tgtaatgcag tcgaaattgg catcgtttta
    64441 gaaacaccaa tcaagttgaa agatgtatac tgtcctgttt gtccaataac ccaaaatgca
    64501 ccagaaagta aactaatcaa aaatgtagtc actgaaatac cagctggaga agcgaataat
    64561 tgaacgagta agccgacaag caccgctcca ataccagttc ctaatatctc atttgttggt
    64621 ctgccaccaa tctttttcaa aatcagtggc tggatccccc agccaactgc cgggatcaat
    64681 gcaatcaata aacccatttt tattcaccct tatccttttc caccttggaa agaaggatat
    64741 aaagtcatcc caccatctgc aaatagtgta atacctgtaa cataactgga ttcgtctgaa
    64801 gccaaccatg cagctgtcgc cgctatttct tttggttcac ctacgcgtcc tagtgggatc
    64861 atactttcca agagttcttt ttgttcagga tcatcaaatt ttgccgcatt aataggtgta
    64921 cggatagcac cagggcctat actattgatt ctgattccct tcggtgcata ctccattgct
    64981 accgtttcgg taaataattt taccccgcct tttgaagcag cgtaatgagc aaacgttggc
    65041 catgggatct gttcatgaac agaagacata ttgatgatat ttcctttttt attattctct
    65101 gtaaagtaat ttaaagccgc tttcgttccg ataaaaacgc cagtcaaatt gacttttaac
    65161 actctttccc aatcttcaag gctaagttca tgggttggat gctgattttc catcccagca
    65221 ttattaaccc aaacgtccag tgtcccaaaa ttttctaaag ccgtatcaag taaacgctgt
    65281 attccttctt cagaactgac atctgcttgt acagagactg cttttccgcc tgcctcttcg
    65341 attgctttga ctgctttttc tgctccttct ttatccgagt tatagttgac aactacattc
    65401 attttttctt tcccgaatcg ttgagcaatc gctgttccaa tcccctttga gccaccagtt
    65461 actactgcga ctttgttttc taattccttg tacatagtac ccactccttt ttcataccta
    65521 aacctgttat tacggataac ttttatttcc gccttaaagt gagtgtacac caagatatat
    65581 agtctaaagc aaatgatacg ctctagcaat cgttttcatg aattgctagt gtccttttat
    65641 ttctataaag aaagaggttg tgacagaagt ggtcagcttc aagaaatttc ggcttatttc
    65701 cgaagaagct acttctgaaa aaccatttat ttggaaaaaa aggctgtgac aagacttttg
    65761 tcacagcccc tttcctgggc ttatttatca cccattttat tgtaattgct agttatacat
    65821 aaacaagaga ttttatgcat aatgttttat tgctgagaaa aatattcagc tgttgcttgt
    65881 tcatcctcct tgagttgttc aatcaattcc tctacactag aaaatttcac ttgatctctt
    65941 aaaaattgat tccagcgtac cgcgacatgt tcaccataga tatcttgatt aaagtccaaa
    66001 atatacaact ctacagtcag ctcccgtcct tcaccgaatg tatcattatg tccaattgaa
    66061 cccatggctg gataccatgt ttccccaatt tttatttccg acacataaac accttcttta
    66121 ggcaaatgaa ccgtgctctt tactttgaca tttgctgtag ggaatcctag tagtctgcct
    66181 cgtgcatcgc catggacaac taccccttct acctcatatg tgcaacctaa tagttttgct
    66241 gcttctttta cttgtccgct gtctagtaat ccacgaatac gggtagagct gattttttca
    66301 ccttgttctt cttctttaga aactgtgacg attttgaatc ttcctttggc atattcagga
    66361 aaatgagcga catctgcaat gtcttttggt ccatacgtat aatcaaaacc ggaaacagca
    66421 tattttgcat gtaaccctac gatgtattga tcaacaaatt cctgcggtgc taaatgagca
    66481 aaagaagaag taaaatcaac ttcatataaa taatctatac ccaaagcggc cattttttct
    66541 tctttttgct taaggctagt caaatatttc acgctgctag gatcgatctt tttaaagaca
    66601 atagatggat gctgattgaa agtcatcaat gctaatttca atccttcttt ttgtgcaatt
    66661 tccatacctg tctttatgac tttctggtgt ccgatatgca cgccgtcgaa aaatcccagc
    66721 actaaaacga catcgtcttc tggaatttgt tctgattggt aaggatgtct gatcttgatg
    66781 atattcattc gttattcctc atttctcaat actttactag gttttaattt gtttttttct
    66841 ttcgggtttg gctgataaat actgacaact tttcctttat aaaacaaagc aatttcttct
    66901 gacggcattt ctgggagcgc aaatacagta taatcaagtc tcataccgtt ttttactttc
    66961 tgccatactt catcagtaat atctactcgt ttgaaccgtt ccactccttt ttctattggc
    67021 agcaaacaat catcgattgt cccatttttc acagcttgag ctacttcttc taatgtcaga
    67081 gcatccttct gggacatacc ggcacttgct gttcgggtca gatcagacat gtgcgcaggg
    67141 taaccaagtt tcttgcctgt atctacagct aatgtgcgga cataggttcc tttcccgcat
    67201 tctacttcga agcgccaaga aatggttcct gtcacttcgt ctcttgtcaa ttcacttgtc
    67261 cggataaaac ggtaaatctg agcctttcta acaggacgct ccactgtttc attattgcgg
    67321 gcatattcat ataaccgctt accgttgact tttaccgctg agtacattgg tggtatctgg
    67381 gtgatctcac caataaatga agccatcact tcatctattt ttttttcaga taaagacata
    67441 tctactggtt ttgtttctac ccgttctccg gatcgatctt ccgtcgttgt actgaaacct
    67501 agagttatct cgcctacata gattttccca gaatctgtca aatattcgat aacttttgtg
    67561 gcttttccaa tacaaatggg caacacaccg tctacgtctg ggtcaagcgt acccccatgt
    67621 ccgatttttt tcgtatgtaa gatttttctt aatttgaata cgcaatcatg acttgtcatt
    67681 ccgcgttctt tccataaagg taataacccg tccatgtctt ttctccattt catttttgat
    67741 ttaactgaat tattataaca cattgcatca attactgacg aatttgacat cataatgaac
    67801 ggtttttatt tttcggcaat aaaaacgtaa caaaattcat gctcacgtaa atagaaatca
    67861 tgttatgctt ttttaaagga gggaatagaa tggaagattt tttctttgca ccatttgatt
    67921 ggatttatgg gtggcttaca ggtaaatagt ttggttttca cttcccaaaa acgaaaaaaa
    67981 agactggcca ttcttccagt cttttttccg ttttttttat ttattttttc agtttcttaa
    68041 tcaatgtaaa aaattctatt ttgcgggatc gattttatca ttttattggt caagattttt
    68101 ccatttccaa tccaatactt ctggcagatc aataccattt tcacgaatat agttttgatg
    68161 ttcttgtaat ttctgatcca tttttttcgc aaaatcttct gcttgacttc ctttgatttt
    68221 taaagctgcg tcttttgcca aatgataacg atcaagttca tttactacac gcatgtcaaa
    68281 tggtgtcgtg atatctccat tttctttgta gccatgaatc attaaatgat ggttatgacg
    68341 atcaaagaaa atcgtgcgga ccaatgtttc ataaccgtgg aaagcaaata agatcggttt
    68401 gtcttttgta aaatattgat caaattcttc atcgctcaac ccacgcggat cttgacttgg
    68461 atgacgtaat ttcaataaat ctacgacatt gatgaaccga atttttagtt ttgggaattc
    68521 ttccagcagc aaagttacag ctgctaatgc ttctaagtta gattccgttc ctgcagcagc
    68581 aatgaccaat tcaggttcct cctcttttgc tgtcgaagcc caatcaatga ttttcaagcc
    68641 ttcattcact aagacggctg cttcttctgc tgagtaaaat tgagcacgcg gatgtttgga
    68701 agagatgatc aaattgattt tttctgacga gcggaatgct ttatccatga ctgcgagcaa
    68761 tgtatttgca tccgcaggca gatattcacg gatgaactcc gccttctttt ctgccaaatg
    68821 tgtcaatatt cctggatctt ggtggctata tccattgtga tcttgctgga agactgttga
    68881 gcttgcaatg agatttagcg aaggataatc atttcgccaa gaaaggtcat gtgatttccg
    68941 catccatttg aagtgctgag tcaacataga gtcaaccact cgcaagaatg attcgtaact
    69001 tgcaaaaaac catgccgtcc agtcagaacg tacccttcta agaacccttc atcttggtgt
    69061 tccgataatt gactgtcgat gacgcgacca gatggcgaaa gccattcatc attttctggt
    69121 tcgatttttt ccatccattg gcgatttgtc gttttgaaaa cttgattcaa tcggtttgat
    69181 tttgtttcat ctggtccaaa tatacggaaa ttttctggat ttttctcaat gatctcgcga
    69241 acgaatttac caagttcggt catatcttct gctttaacgg agcctggttt ttcaaattgc
    69301 aaagtatatt ttttccaatc tggcataata agcggtcttg gatcgattcc accattggtg
    69361 ataggatttt ttgccattct ttggtcacca gtaggcgcga tttctctgat ttcttctaaa
    69421 atacgtcctt gtgcatcaaa taatttttct ggttcatagg atttcagcca acggagtaaa
    69481 gcatctgcat gatccatatg ttcttgatct acaggaatcg gtacttggtg tgctcggaag
    69541 gttccttcaa ttggttcccc gtcccattct tttggaccgg tccatccttt tggtgtgcgt
    69601 acgatcaata ctggccattt tggcatcacg acatctgttg ctgtcttcag gcgagcttct
    69661 ttttggatgg atttaatttt ctcgattgct tgatccattt tttcagccat aacaggattt
    69721 aatttttctg aatcatttcc ttcaataaag aaaggttccc agccaagtcc gttaaagtaa
    69781 tttgctaatt ctccatctga tttacgtgcc aagatcgttg ggttggctat ttttgcacca
    69841 tttaaatgaa ggatcggtaa aacagctcca tctatcactg gattgataaa gacatttgag
    69901 aaccagcttc cagctaaagg accggtttct gcttcaccat caccaatcac gacagcagag
    69961 attacttcag gattgtctaa tacagctccg actgcatgtg aaagagagta tccaagctct
    70021 ccgccttcat ggatagaacc tggtgtttct ggtgctgcat gagaagcaat gcctccagga
    70081 aatgaaaatc gtttgaataa tttttgcatt cctgtctcat cttccgttac ttctggatag
    70141 atctctgtgt aacttccatc taaataagca ttcgatacca tgacttgtcc gccgtgtcct
    70201 ggcccttcaa tataaaacat gttcaaatca tatttattga tgactcggtt caaatggaca
    70261 taaataaaat tttggccagg aatcgttccc caatgaccga ttggatgttt tttgacatct
    70321 tcgggtttca gtgtacgtct cagtaagggg ttatctttaa gataaagttg cccaacagaa
    70381 agataattag cagcacgcca ccaagccgtc attttatcaa agtattcttt actagaataa
    70441 tccatacata acactccttt tcattattac tcttattcta gcgcagatac tcccaattga
    70501 aacctaataa gcacttctgt tcatttgttt ggattttata tctagccaaa tgaacaggta
    70561 taaaaaacca cgaaattttt cctttcgctg acgtttttta cattgatcca aaaaacttgt
    70621 cgttatcaca atggctgttc cgcagttcat ttcgctcctc atcatgcata atttgctgaa
    70681 tgacgctggg ccagttaaca cattgctgga aaactggggc tggatcgagc aattcctttc
    70741 ctgattgatc cgttttatgt aaaagtctct gttatctttg tcaatatgtg aattggtatc
    70801 ccagctacta tgctaatttc tacagggatt atccaaaatt tggatcgtga ccaaatcgaa
    70861 gcggcgtaat cggaaatatc aataacttca acgtgatttt tcttttaact gtcaacggac
    70921 cgacaaactc aaacttttat ggtgcaggtt ccactgatct tctagttact tggctatacc
    70981 tgactgtcac taaaatagat tacaatctag cttccgttat cggtatcttg atctttatct
    71041 atatgcaaaa atttatatac tactagaaaa aagaaagtcg aattttttac ttttcttttt
    71101 ctcttttttg gttagctgac acgccgcaag actaaagtca tggaactctt aggtaatacg
    71161 tcttaatgaa aacaaaaaaa tcccgcaacc cctg
//
LOCUS       DASHHB010000008        65527 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.008, whole genome shotgun sequence.
ACCESSION   DASHHB010000008 DASHHB010000000
VERSION     DASHHB010000008.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65527)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65527)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65527
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.008"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            209..1072
                     /locus_tag="U2K71_000573"
     CDS             209..1072
                     /locus_tag="U2K71_000573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM7314682.1"
                     /translation="MYQIVTDSCCDLPYHVLAKEKVDFLSMNLQLNDQELVDDLGKTF
                     NYDRYMNQLKNGAMPTTSQINVGRYLEFFRSYAEKQIPVLYVAFSSGLSGSYSSALQA
                     VELLKEEYEDPEIYVFDTKAASIGEGLLVLEAVRLKKEGKSIEETLEWLESNYLKVHS
                     WVTVDDLKYLERGGRISKASATLGSLLSVKPIITVDKEGRLTNVDKVRGRNKSIQTIV
                     NETVKTIVAPLDQIVYVAYAGDLESAEKAKRLLEEQIKMKDVKLYPLGPTIASHTGYG
                     CIAIFSMGISR"
     gene            complement(1201..2823)
                     /locus_tag="U2K71_000574"
     CDS             complement(1201..2823)
                     /locus_tag="U2K71_000574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7314683.1"
                     /translation="MITVNDVSLHFSDRKLFDDVNIKFTPGNCYGLIGANGAGKSTFL
                     KVLSGDLQPSTGSVTMGPDERMAVLKQNHYDYEDYTVLETVIMGHKRLYEVLKEKDAI
                     YMKEDFTDEDGIRAAELEGEFAELNGWEAEPEAAVLLQGLNIPEELHHQQMSELTGGQ
                     KVKVLLAQTLFGKPDVLLLDEPTNGLDIQSINWLEEFLINFENTVIVVSHDRHFLNKV
                     CTHMADLDFGKIKLYVGNYDFWLESSQLAAKLQANANAKKEEQIKELQEFIARFSANA
                     SKSKQATSRKKMLEKITLDDIQPSSRRYPFVGFKPEREIGNDLLQVENVSVTIDGKKI
                     LDDISFNLSKEDKVAFVAENDITTTTLFKVIMGEITPDTGTVRWGVTTSNSYLPKDTT
                     KEFDTDLTILDWLRQYASKEEDDNTFLRSFLGRMLFSGEEVLKPVNVLSGGEKVRCML
                     SKMMLSKANVLVLDDPTNHLDLESITALNDGLMAFTGSILFASHDHQFIQTLANRIIA
                     VSDKGVIDRADTTYDEFLENADIQKQLNELWA"
     gene            3072..3593
                     /locus_tag="U2K71_000575"
     CDS             3072..3593
                     /locus_tag="U2K71_000575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEM7314684.1"
                     /translation="MDEKRTIDWGSLILGILFVLVSLLSFQDPVGNLVAIVVVFAIFA
                     FIKGIFELFVRNRLKELTGYKGKMPLIIGIIDILVGVFFLFNIGAGVAALPFVFAVWF
                     IADSVLALFTADLARGVSEGYYWFTIIVNILGILLGIFLLFNPISSALTLSFLVGFYF
                     MLFGITHIVYAFR"
     gene            complement(3779..5509)
                     /gene="spxB"
                     /locus_tag="U2K71_000576"
     CDS             complement(3779..5509)
                     /gene="spxB"
                     /locus_tag="U2K71_000576"
                     /EC_number="1.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate oxidase"
                     /protein_id="HEM7314685.1"
                     /translation="MSKINAGIAMVKVLESWGIDHIYGIPGGSFNSIMDALYQEKTEI
                     NYIQVRHEEVGALAAAADAKLTGKVGAVFGSAGPGATHLINGLYDAQMDHVPLVAFLG
                     QVASTSMNYNSFQELNENPIFADVSVYNRTVMTPQSLPHVVDEAIKAAYEHKGVAIVT
                     IPVDLGFEEIDEQPFSTAHTHKTSVILPEEKDLLAALPYLEKAKKPVLYVGQGTRNGF
                     PQIKEFSEHFSVPVVASVLAKGIIPDDYENFLGFAGRVATKPGNEALAEADLILFVGS
                     DFPFGRAFFNPTAEFIQIDIDASKFGRRHETSLSILGDANTALQRLVELGDARPADSW
                     YLANQENKEKWVNWLKSFEDREEEPIRPEAVYKEINRIAEDDAIFVTDVGNTTIHSIR
                     LLNMNGKQKHTTSGWFATMGNGVPGGIAAQLSYPEKQVFTLSGDGGFAMVMQDIITQV
                     KYQLPIINVVFSNDSFGFIEAEQEDTEQKKFGVFLEDADFGKVGEALGANSFTVTEYS
                     QLRFAFDAAAKSDRPVVIDVKINNKRPLPVEDLHLDPRKYSEEEIQAFKEKYEVHDLP
                     VLNELYEKRL"
     gene            5674..6492
                     /locus_tag="U2K71_000577"
     CDS             5674..6492
                     /locus_tag="U2K71_000577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ZIP family metal transporter"
                     /protein_id="HEM7314686.1"
                     /translation="MVEWLLRLEPWQQALAGTAFTYFMTALGAGLVFFFKEIKKEVLN
                     LMLGFASGVMIAASFWSLLDPAITKAEENGDIAWLVVSIGFGLGGVFLYMADKTLPHM
                     HFGPQHEAEGLPTHLKRTILLVFSITLHNIPEGLAVGVAFGAAATADNPTAAILAAVS
                     VALGIGIQNFPEGAAVSIPLRQEGLSRKKAFVYGQASGIVEPIAGVIGALLVTKVELL
                     LPYALAFAAGAMIYVVVEELIPEAQQTLSSKHHFAVFGVMSGFIIMMILDVALG"
     gene            complement(6595..6792)
                     /locus_tag="U2K71_000578"
     CDS             complement(6595..6792)
                     /locus_tag="U2K71_000578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314687.1"
                     /translation="MRQKNMFLNIFMVLLVLIAASFVISLFVSVLSGLLWFAVKVLIP
                     IALIIWLVRWIKGNTHRKNYY"
     gene            6961..7539
                     /locus_tag="U2K71_000579"
     CDS             6961..7539
                     /locus_tag="U2K71_000579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEM7314688.1"
                     /translation="MPKNRLEAFTDAVIAIIMTILVLELHEPNGDTWEALAALGYRFF
                     IYIVSFIILAIYWNNHHHLFQVVHKINGRVLWANSFFVFALSLFPFATSWISEYINSL
                     APEMTYGFVTLTANIAYYILAKELVKANKGHEKALKAMLNNYKKSYLSIGLNVLGIVL
                     GYLVAPVAVLIANIVILAAWIIPSRTIESQYR"
     gene            7634..7771
                     /locus_tag="U2K71_000580"
     CDS             7634..7771
                     /locus_tag="U2K71_000580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314689.1"
                     /translation="MDQKRIHVGLITSFVTMLIYLDQGHLTWTVVGVVGFLTLFTAIK
                     K"
     gene            complement(8584..9087)
                     /locus_tag="U2K71_000581"
     CDS             complement(8584..9087)
                     /locus_tag="U2K71_000581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEM7314690.1"
                     /translation="MRYVSKFLLGIYLFFLLWLILFKLSFNLPQFLTYSYSNVNLVPF
                     STFSFENTTVLRETTYNLIVFFPFGVLLNVNFKRLSFSKKLGIIFLVSFLAELIQFLF
                     GIGVADITDLITNTTGGLIGLWAYQLLNKHLSTNKLDRLAIILGYILFLFLIVAFLFL
                     TRTVRLQ"
     gene            9446..10471
                     /locus_tag="U2K71_000582"
     CDS             9446..10471
                     /locus_tag="U2K71_000582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM7314691.1"
                     /translation="MFKTYFRFNKIQDNPFRIMNLMLLIFIVLWLGYINHDFLISSFS
                     SLGIFTFFYYQNIPMKYLLKRMFLIGCGLLVAFVLGILSTYVLWLEPFAVALVAFSSR
                     FVLRLFHISKPGGLFFAMLSAMGTSMQLPIAQLPIVSLYFFMGVVFALIAAVITKLLD
                     SRPEQTIEKATLKERFHEEPLVIIDSVFYSAALFLSVYVSHGLNLHNPYWLTLSCASI
                     LLAENLDAMKHRQVQYLIGSMGGLCVSAFLSFVPFTQLQTIFLITFLYGIAQFLVARN
                     YAVANIFLNPMALMLSTLIRGAYLISLIEYRFLGIVIGSFIGLGVAWVMTVGLQHYLT
                     VVRQKLE"
     gene            10794..11672
                     /locus_tag="U2K71_000583"
     CDS             10794..11672
                     /locus_tag="U2K71_000583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5596 domain-containing protein"
                     /protein_id="HEM7314692.1"
                     /translation="MRFKDMKRGNLMKKISNEIQILAETLTLPKVVIEQMIVQAKNIL
                     DEVFEQYSHELMDPNKAKATAKKIDKVYEEEPFAALAIYLYAASQSWKKIYQPLKIPR
                     TIYLATMNAFTRFIHEHFQVTGSYRFDRGFWIWHYTSGLIFRIGELEYEMTYFPKGYT
                     DLRLEGKQCLSLHIPSDADLSQNKIRDSYESAIRFFELYFPDYHYQVMYTDTWLLSPN
                     LTKWLKKESKICLFAADYRLLSVDEQDDSGVPWIFGRVDAQIHDYPESTSLQRQAKEQ
                     LLAGEHIGSGLGILTS"
     gene            12148..12264
                     /locus_tag="U2K71_000584"
     CDS             12148..12264
                     /locus_tag="U2K71_000584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative metal homeostasis protein"
                     /protein_id="HEM7314693.1"
                     /translation="MEKTDISSAYRRLKSPNIKTRKRALKIIKDVKRNSGKR"
     gene            13192..13530
                     /locus_tag="U2K71_000585"
     CDS             13192..13530
                     /locus_tag="U2K71_000585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314694.1"
                     /translation="MTQPVSSEEHDNLFRATEEFKKYQENIVPETPLASEPITDIDGN
                     QIGWYIQYEVMVVNNDSIVKNTVGLELTPIEKVSSLLTYIYTDESDETEIILTDYSKI
                     ATDKKYILWM"
     gene            13624..13854
                     /locus_tag="U2K71_000586"
     CDS             13624..13854
                     /locus_tag="U2K71_000586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314695.1"
                     /translation="MLVEKGKENIYYVNVAKVREDENEWKEFKSRYSINSTPTFTVYR
                     EGSIEKTVFWTKESGMSLAEVEEFLDYVSMQQ"
     gene            14254..14625
                     /locus_tag="U2K71_000587"
     CDS             14254..14625
                     /locus_tag="U2K71_000587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cro/Cl family transcriptional regulator"
                     /protein_id="HEM7314696.1"
                     /translation="MRDYVTDKTQEVINQSQSLNELSDKAKEEAVAIISSGISDYIDM
                     SGEINYGDKDKIVRMIKAFIKTFDVKADLKFNDTYLVGKLINILGTEKDTETFISSLS
                     MLFTDKHFSTQLGGEELVEIF"
     gene            complement(14899..15591)
                     /locus_tag="U2K71_000588"
     CDS             complement(14899..15591)
                     /locus_tag="U2K71_000588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM7314697.1"
                     /translation="MKGIIFDFNGTMFLDSHLHEAAWLHMIHDHTKDGLSDEDILKNI
                     HGRTNTEILKHFISEHLTASEIAVLSEEKESYYRMLCLQNENELQLTKGLEATLDQLT
                     DQQIPLTIATATVKENVAFYFDIFDLARWFDWDKIVYDDGSFPGKPQPDIFLKAADKL
                     SLAPADCLVIEDAYSGLLAAKRAGIGTIIAIDPFGKNKTVFEQAQLNKDGTIKDFTGF
                     WTEHLSATSVNS"
     gene            complement(15594..16379)
                     /locus_tag="U2K71_000589"
     CDS             complement(15594..16379)
                     /locus_tag="U2K71_000589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM7314698.1"
                     /translation="MLKKERLLTIVEMVNKKGILTVNEIINQLDVSDMTVRRDLDELE
                     KSGKLLRVHGGAQSLSYTLDQELSHTEKATLQIQEKKEIAATAASLIHEGETIFLGPG
                     TTIELLATHLLNKKIRIITNNYPVFDILAQADGPEILLIGGDFRKNTGAFVGPITNDN
                     LRRLKFTKAFISSNGVHNEAISTYSTEEGEAQQIALNNSRKKYLLVDNKKFNRDDFYV
                     FYNLHDFDLLITDRQISREVQEHYAQYVDLQIADSTENTTIGA"
     gene            16616..18016
                     /gene="lacG"
                     /locus_tag="U2K71_000590"
     CDS             16616..18016
                     /gene="lacG"
                     /locus_tag="U2K71_000590"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEM7314699.1"
                     /translation="MLKLPEDFIFGGATAAYQVEGATKEGGKGAVAWDDFLEEQGRFS
                     PDPASDFYHQYAKDIELCERFGVNGLRLSIAWSRIFPDGAGKPNPEGIAFYHRVFEEC
                     KKRNVTPFVTLHHFDTPKRLFDHGDFLNRETIEAFVSYAIFCFHEFKEVKVWSTFNEI
                     YPVATNQYLLGVFPPGIKYDFTKIVACLHNMMVAHARVVNYFKENELPGEIGVVHSLE
                     TKYAATDAPEDKHAAFLDDALSIRFLLDATYLGYYSTETLTALDEICEANQASYHFPE
                     EDFVELKKASTRNDYLGINHYQCHFVKAYDGENAIHHNGTGEKGTSVYKVKGIGERIY
                     KEGIPRTDWDWLIYPEGLYDLLLRIKSDYPHYNKIYITENGMGYKDQFEDGIIMDQPR
                     IDYLRVYLESLSKAITAGVNVKGYFLWSLMDLFSWTNGYNKRYGLFYVDFETQKRYPK
                     ESAYWYKLVSETKTII"
     gene            18041..18358
                     /locus_tag="U2K71_000591"
     CDS             18041..18358
                     /locus_tag="U2K71_000591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010749967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM7314700.1"
                     /translation="MNKEELQMLGFEIVAYSGDARSTLLKLLKEVRQGNFEHVDSALK
                     EADENLTLAHNSQTKILAEEASGKEMEMGFIFIHGQDHLMTTLLLRDLIQDFIVLYRQ
                     NQG"
     gene            18371..20044
                     /locus_tag="U2K71_000592"
     CDS             18371..20044
                     /locus_tag="U2K71_000592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEM7314701.1"
                     /translation="MDGIIKQIEKGKPFFEKISRNSYLGAVRDGFLTAMPAILFSSIF
                     ILIAAVPEIFGYQWPESVSTWLWKVYNYSMGVVGLLVSATTARCLADSMNRRLPKNKV
                     INTTSVMLASVVSFLLLSVSPIEGGFSTEYMGTKGLLASFVAAFLTVNLYKFCVLKDI
                     TIKMPKEVPGTISQTFRDIFPFSFSVFAAVLIDLGIRQIFQISFAEAIITVLQPLFTA
                     ADGYLGIAIIWGAMALFWFVGVHGPSIVEPAIAAIIYANVETNLQLFNSGEQASKVLT
                     VGLGNFVGTMGGTGATLVVPFIFLLFAKSKQLKAVGKASFIPVSFAVNEPLLFAAPMI
                     LNPYFFIPFLLTPVVNVCLFKFFVDVLGMNSFMYVLPWATPAPIGLVLGTGMGILAFV
                     LAAVLIVVDFLIYLPFCKAYDAVLVEKEAQKAEVIATEEGASADVREEVPVAASQVIS
                     DANKEINVLVLCAGAGTSAMLANALKKGASESKAPISAMAGAYGSHYEMLQDFDMVVL
                     APQVNSYYDDIKEDTDRLEIKLVATKGAEYIGLTRDPEKAVQFILNQFQ"
     gene            20058..21689
                     /locus_tag="U2K71_000593"
     CDS             20058..21689
                     /locus_tag="U2K71_000593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HEM7314702.1"
                     /translation="MKMGIWLRKAVLFAAVLGALGAVSKTVEASASEFIRGADISILS
                     DMEKSGAKYYEDDISKDALEILKNNGVNYVRLRLWQDPYNSNGESYGAGTNDLETTID
                     LAKRAKNLGLKVLLDFHYSDFWVDPGKQNLPKAWQGLTFEEMNTALYDYTKNVLSEMK
                     QLDVYPDMVQIGNELNSGMLWPYGKSWGEGGGEFDRLAAFLKSGIQAVRDTQPKTTPV
                     MLHLADGGDTGAFTWWFDEITSRGVSFDLIGVSYYPYWHGSMNELQTNMNAISQRYGK
                     EVIVVETAYGHTTANADTMPNAFGEAEAAAGGYEPTPTGQAEYLLDLADRIQAVPNNR
                     GAGFFYWEPLWYNGNVSWATQAGMNYLSVQSTMGNEWDNQAMFDFAGNALPSLRAFKQ
                     AGAQINLVKNASFESDGPTTSPSNWQTWFQQGTAKGTIKTEAGTFFGDYKLTFWQDQA
                     YEASAYQNVSGISKGTYTLSAYVMSGGEFDTNQLYIKNYGGAELNTAVVKTSEWTKIT
                     IKDIPITTGVCEIGVYSKASGNAWTNVDFVSLVKQ"
     gene            complement(22180..23745)
                     /gene="guaA"
                     /locus_tag="U2K71_000594"
     CDS             complement(22180..23745)
                     /gene="guaA"
                     /locus_tag="U2K71_000594"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HEM7314703.1"
                     /translation="MTNVADMTNVEKIIVLDYGSQYNQLITRRIRDLGVFSELLSHRI
                     TAEEVKAMNPKGIILSGGPNSVYDENAFGIDPEIFNLGIPVLGICYGMQLITYKLGGS
                     VEPAKNREYGKALLEVTSDQAVLFKETPKQQTVWMSHGDLVTKVPEGFETVGTSKDCP
                     IASIQDTTRNFYGIQFHAEVRHSEYGIELLRHFAFDVCHCEGNWTMDNFIDMQVEKIR
                     EQVGDKKVLLGLSGGVDSSVVGVLLQKAIGDQLTSIFVDHGLLRKGEAEQVMESLGGK
                     FGLNIIKVNARDRFLDKLKGVSDPEQKRKIIGNEFVYVFDDEATKLAGEEGVSFLAQG
                     TLYTDVIESGTETAQTIKSHHNVGGLPEDMQFELIEPLNTLFKDEVRELGTQLGMPDS
                     IVWRQPFPGPGLGIRVLGEITEDKLEIVRESDAILREEIAAAGLDRDIWQYFTVLPGI
                     RSVGVMGDGRTYDYTVGIRAVTSIDGMTADFARIPWEVLQKISVRIVNEVAHVNRIVY
                     DITSKPPATVEWE"
     gene            complement(23804..24727)
                     /locus_tag="U2K71_000595"
     CDS             complement(23804..24727)
                     /locus_tag="U2K71_000595"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HEM7314704.1"
                     /translation="MNDPMNYYRVAREEWREFYRNGQAPLTQDELDNIKSLNDRISMQ
                     DVRDIYVPLAHLIYLYMKEFESLFLSKGLFLHKYVEVPPFIIGIAGSVAVGKSTTARL
                     LQTILSRTFKRRNVQLITTDGFLYPNRILKERGIMDRKGFPESYDMEMLIDFLNRVKS
                     GQEEIKVPVYSHDVYDIIPDKYELIQQPDILIVEGINTLQLPANQQIYVSDFFDFSVY
                     VDADPVLIEKWYLDRFGALLDTAFQNPENYYYRFAIGDREEAFKMAKDVWKNVNLKNL
                     QEYILPTRGRADIILHKTENHIIDSIFMRKY"
     gene            24923..25528
                     /locus_tag="U2K71_000596"
     CDS             24923..25528
                     /locus_tag="U2K71_000596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM7314705.1"
                     /translation="MTNHYYSERPDTAHDFEQWAFELKGRTFQFVTDSGVFSRETVDY
                     GSRVLIDAFDWEELPDEGKILDVGCGYGPIGLALAFATQRFVEMVDINERAVSLAQGN
                     AKRNQIEHVDIHQSNIYEAVHEETYAAIVSNPPIRAGKKVVHEILTGAYPRLKKGGTL
                     TIVIQKKQGAPSAQKKMEETFGNAEIVTKDKGYYIIKSVKE"
     gene            25836..26852
                     /locus_tag="U2K71_000597"
     CDS             25836..26852
                     /locus_tag="U2K71_000597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEM7314706.1"
                     /translation="MKLTIRDIAEMAGVSVTTVSQILNNKGSRFSDKTRKKVLSIVNE
                     HHYKPDYFASNVINRHSKTIGMIVPDVTDFFFSKLIEGVESYLNPLGYVIMLCNSRHS
                     QENEIKYLQELSHRSVDGILLATPNILPEKYALDSGFYQKMPIILIDRGLNRRDNGRL
                     IVKEYEGAYQAVSLFIENGHTKIGMLKETTGYYQLEERFNGYRHALKDHGLPFNGKFV
                     EHGELTVQGGYTASKKLLKHKDITAIFCGNDAMAIGCYQAIDELGKKIPEDISVIGFD
                     GLKLSEYMIPQLTTVQQPSFDIGFYAARFLIDTIEFPQRKVPNKVFETKLIIRESVKV
                     LTKG"
     gene            26993..28294
                     /locus_tag="U2K71_000598"
     CDS             26993..28294
                     /locus_tag="U2K71_000598"
                     /EC_number="2.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine-nucleoside phosphorylase"
                     /protein_id="HEM7314707.1"
                     /translation="MRMVDLIEKKRDGNELSKEEIEYIVTNYTNGKIPDYQVSALLMA
                     IFYQDMTNEEITNLTLAIANSGDVIDLSSLEGIKVDKHSTGGVGDTTTLILAPLVASV
                     GVTVAKMSGRGLGYTGGTLDKLEAIPGFQIELSDEEFVRIVNESKVAVIGQSGNLAPA
                     DKKLYALRDVTATVDSLPLIASSIMSKKIAAGADAIVLDVTTGDGAFMKNIEDARRLA
                     KTMTSIGKLANRETVAVISDMSEPLGEAIGNSLEVVEAIETLQGNGPEDLVEMCYALG
                     SQMVVLAGKAKTTDEARTLLQEALESGKALAKFKEMIQNQGGDPAIVEHPERILTARY
                     TMELPAKQSGVVSKIVANELGIAAMMLGAGRKTKEEDIDHAVGLKLHKKIGDTVTKGE
                     SLLTIYSNDEEITSVIELLYKNIEIGESAMKPTLIHDIITE"
     gene            28340..29002
                     /gene="deoC"
                     /locus_tag="U2K71_000599"
     CDS             28340..29002
                     /gene="deoC"
                     /locus_tag="U2K71_000599"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HEM7314708.1"
                     /translation="MELNRMIDHTILKADASKEAVMQIIEEAKKYHFYSVCINPAWVA
                     LAKEQLQGEPVAVCTVIGFPLGANTPETKAFETTDAINNGADEVDMVINIGALKSKDD
                     QQVQKDIEAVVEAAKDRALVKVIIETSLLDREEIIRACEIAKAAGADFVKTSTGFSTG
                     GAKVEDVRLMRETVGPEMGVKASGGIHNEEEAMAMIEAGATRIGTSAGVAIVSGSTGE
                     GY"
     gene            29023..29421
                     /locus_tag="U2K71_000600"
     CDS             29023..29421
                     /locus_tag="U2K71_000600"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HEM7314709.1"
                     /translation="MAELKQEWINTAIEALDKAYVPYSHFPVGACLVTESGKIYQGIN
                     IENASFGLTNCAERTAFFKAVSEGEKTFTHLVVAGHTPDPISPCGACRQVMAEFCSPD
                     MPVTLVGDNGVTKATTVRALLPYAFTEKDL"
     gene            29487..30569
                     /locus_tag="U2K71_000601"
     CDS             29487..30569
                     /locus_tag="U2K71_000601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM7314710.1"
                     /translation="MKKAKLFGLGAVALAAGLFLGACGNNGSTDSSGGKSSSDTTTAA
                     LITDTGGVDDRSFNQSAWEGLEKWGKDQGLSRGNDGFQYFQSSNESDYIPNIDQALNA
                     GFKTIFGIGYKLKPAIQEQATNNTGTNFVIIDDVIDGLDNVVSATFKDNEASYLAGVA
                     AAYTTETNVVGFIGGVKGEVIDRFDAGFKAGVDAGAKELGKEIKVLNQYAGDFSAPDK
                     GRSIAQGMYAQNADIIFHASGGTGNGVFQEAKSLNESGDKKVWVIGVDRDQSDEGEYT
                     LNGEKKNFTLTSTLKAVGTVVEDLAQKSADGKFPGGEHTVYGLKEDGVGLTEGQLSDE
                     AKKAVDEAKEKIISGDVKVPETPEEN"
     gene            30781..32343
                     /locus_tag="U2K71_000602"
     CDS             30781..32343
                     /locus_tag="U2K71_000602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314711.1"
                     /translation="MVQENIVIEMQNITKEFGTFKANDNINLQVKAGEIHALLGENGA
                     GKSTLMNVLSGLLEPTSGKILMRGKEVKITSPTKANQLGIGMVHQHFMLVDAFTVTEN
                     IVLGSEPSRAGMLDHKKARKEIQKVSEQYGLSVNPDAYVRDISVGMEQRVEILKTLYR
                     GADVLIFDEPTAVLTPQEIDELIVIMKELVKEGKSIILITHKLDEIKAVADRCTVIRR
                     GKGIGTVNVKDVTSQQLADMMVGRTVSFKTMKKEAKPQEVVLSIENLVVKENRGLEAV
                     KNLNLEVRAGEVLGIAGIDGNGQSELIQALTGLRKAESGHIKLKGEDITNKKPRKITE
                     HGVGHVPEDRHKYGLVLDMTLSENIALQTYHQKPYSKNGMLNYSVMNEHARELIEEYD
                     VRTTNELVPAKALSGGNQQKAIIAREVDRDPDLLIVANPTRGLDVGAIEFIHKRLIEQ
                     RDKYKAVLLISFELEEILNVSDRIAVIHEGEIVGIVDPKETSENELGLLMAGYTLEEA
                     RKELEKGAGAHE"
     gene            32336..33469
                     /locus_tag="U2K71_000603"
     CDS             32336..33469
                     /locus_tag="U2K71_000603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314712.1"
                     /translation="MNDRSEKFRNLLVPVLSVVLGLILGAILMAAFGFQPIQGYSSML
                     DAALGSQRGIGETLRQATPLIFTALGFSVANSAGFFNIGLSGQALCGWIVSVWVALAF
                     PDLPKVVLLPLCVILGALAGAMAAAVPGLLRAFFGTSEVIVTIMMNYILLYVSTFALH
                     EVMPESYRSSLDSSNMISQNASLRLPWLTQAFGGSRVNSGLFLAIIALIFVWLLMKKT
                     TLGFEIRSVGLNPFASEYAGMSSKRTIVLSMVISGALAGLGGVVEGLGTYQNFFVQTT
                     SLSIGFDGMAVSLLGSGSAIGILLSAILFSILKIGGLGMQTGAGVPFEIVNVSIALII
                     FFVGINFLIRFVMAKVFQGKKQEEIVATIESKPTNQNHEGGEL"
     gene            33469..34425
                     /locus_tag="U2K71_000604"
     CDS             33469..34425
                     /locus_tag="U2K71_000604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314713.1"
                     /translation="MSTIELIALILTSTLVYSTPLILTSLGGTFSERSGIVNVGLEGI
                     MVMGAFSSIVFNLTMSPSLGSMTPWIAILVGGVVGILFSLIHAVATVSLRADHIISGT
                     VINLMAPALGVFLIKAWYGKGQTDNITQNLGYFSFPGLADIPVIGPIFFKNTFLPAYC
                     AILIAVLAWVIIFKTKFGLRLRSVGENPQAADTLGINVYAMRYSGVLISGLLGGMGGA
                     VFAQSISGNFSAGTIVGQGFISMAAMIFGKWNPLGAMGASLFFGFAQSLSIIGAQLPV
                     ISSIPPVLLQIAPYLLTIIVLVVFLGKASGPKANGKNYIKSK"
     gene            34524..35690
                     /gene="deoB"
                     /locus_tag="U2K71_000605"
     CDS             34524..35690
                     /gene="deoB"
                     /locus_tag="U2K71_000605"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HEM7314714.1"
                     /translation="MFKRVHLIVMDSVGIGEAPDADKFGDVGSDTLGHIAKEAGLNVP
                     HLEQLGLGTIRPLEGVESVKDHQGYATKLEEVSVGKDTMTGHWEIMGLNIKTPFRVFP
                     NGFPDDLIKQIEEFSGRKVVCNKPYSGTAVIDDYGEHQMKTGDLIVYTSADPVLQIAA
                     HEEIIPLEELYRICQFVRDITKDDPYMIGRIIARPYVGEPGDFTRTSNRHDYALNPFG
                     HTVLDSLKEAGKDVIAVGKINDIFNGQGITESVRTKSNMDGVDQLLKVMKNDFSGISF
                     TNLVDFDALYGHRRDVVGYAHAIEEFDLRIPELLEAMEEDDLLMITADHGNDPTFTGT
                     DHTREYVPLLVYSKKMKEQGALPQGYYSDISATIAENFSVSSTENGESFLKLLK"
     gene            35714..36532
                     /locus_tag="U2K71_000606"
     CDS             35714..36532
                     /locus_tag="U2K71_000606"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM7314715.1"
                     /translation="MTKLSEMLQETTQFILDKGVKEIDFGLILGSGLGELADEIEDAV
                     MISYDEIPFFPVSTVVGHAGKLVYGSLAGKKVLAMQGRFHYYEGHSMQTVTYPVRVMA
                     GLGAHSVIVTNACGGVNESFVPGDLMLITDHINFTGENPLIGPNEDAMGPRFPDMSQA
                     YTPDYQATAKAAAEKLGLDLKEGVYMGYSGPTYETPAEIRMSRTIGADAVGMSTVPEV
                     IVAAHSGLKVLGISCITNLAAGMQANLSHEEVVETTQRVKQSFKALVKETLTLL"
     gene            36549..37256
                     /gene="deoD"
                     /locus_tag="U2K71_000607"
     CDS             36549..37256
                     /gene="deoD"
                     /locus_tag="U2K71_000607"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HEM7314716.1"
                     /translation="MSVHIEAKEGEIADKILLPGDPLRAKYIAETFLEDPVCYNQVRG
                     MLGFTGKYKGERISVQGTGMGMPSATIYAHELIQSYGVKKLIRVGTCGALSKDVHVRD
                     LVLAQGAATSSSMIEKNFQAFHFPPISDFNLLLKAYEIAKEKGYTVHVGNVLSEDSFY
                     KDDLTETFQLAELGVLGVEMEAAALYYLGAKYHVQTLSLMTVSDHLITGEETTAAERQ
                     STFNEMIEVGLETAIAE"
     gene            complement(37554..38240)
                     /gene="gpmA"
                     /locus_tag="U2K71_000608"
     CDS             complement(37554..38240)
                     /gene="gpmA"
                     /locus_tag="U2K71_000608"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEM7314717.1"
                     /translation="MPKLVFSRHGLSEWNALNQFTGWADVNLAPEGIEEAKEGGRKIK
                     EAGIEFDVAYTSVLTRAIKTCNLILEYSDQLWVPQIKSWRLNERHYGKLQGLNKKETA
                     EKYGDEQVHIWRRSYDTLPPLMEETDEGSAANDRRYAMLDKRDIPGGENLKVTLERAL
                     PFWQDEIAPALLDNKTVLVAAHGNSLRALAKHIEGISDEDIMDLEIPTGQPLVYELND
                     DLTVAKKYYL"
     gene            complement(38412..38993)
                     /locus_tag="U2K71_000609"
     CDS             complement(38412..38993)
                     /locus_tag="U2K71_000609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7314718.1"
                     /translation="MKIIGYARTTINDVDLTTQVDQLSDFGCQEIFHETYEVEQNDST
                     AVHLESVIADMQQGDALVICQLHRLGKSTRQLTELTQLFKDRGLHLISLDEGIDTREM
                     GKIYFQLMENLANMECALIKERTLVGLDEARKKGKIGGRPKIDAKTIKKIRRLYYEKK
                     ETIQFISSKCGVSVGTCYKYINLPEKEIEQLCI"
     gene            complement(39126..39809)
                     /gene="rpiA"
                     /locus_tag="U2K71_000610"
     CDS             complement(39126..39809)
                     /gene="rpiA"
                     /locus_tag="U2K71_000610"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HEM7314719.1"
                     /translation="MNLKQLAGIEAAKFVKDGMIVGLGTGSTAYYMVEEIGRRMREEG
                     LRITGVTTSNATKEQAEKLGIPLKSIDEVPVVDLTIDGADEISADFQGIKGGGAALLF
                     EKIVATYSKETIWIVDSSKLVDKLGKFPLPVEVIPYGSQQLLHIFEEKRFQPVLRTDE
                     NGEVLTTDGGHYIIDLHLEVIEQPESLATYLDELVGVVEHGLFLNMVSKVVVGSEKGV
                     EILDAIRRK"
     gene            40128..40541
                     /gene="rpsL"
                     /locus_tag="U2K71_000611"
     CDS             40128..40541
                     /gene="rpsL"
                     /locus_tag="U2K71_000611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HEM7314720.1"
                     /translation="MPTINQLVRKPRKSKVEKSNSPALNKGYNSFKKTQTNVNSPQKR
                     GVATRVGTMTPKKPNSALRKYARVRLSNLIEVTAYIPGIGHNLQEHSVVLLRGGRVKD
                     LPGVRYHIVRGALDTAGVNDRKQSRSKYGTKKPKA"
     gene            40625..41095
                     /gene="rpsG"
                     /locus_tag="U2K71_000612"
     CDS             40625..41095
                     /gene="rpsG"
                     /locus_tag="U2K71_000612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HEM7314721.1"
                     /translation="MPRKGPVAKRDVLPDPIYNSKLVTRLINRVMVDGKRGIAANIIY
                     NAFDLIKESTGNDPLEVFEQAMKNVQPVLEVKARRVGGSNYQVPVEVRPERRVTLGLR
                     WVVNYARLRGEHTMEERLAKEIMDAANNTGASVKKREDTHKMADANRAFAHYRW"
     gene            41189..43276
                     /gene="fusA"
                     /locus_tag="U2K71_000613"
     CDS             41189..43276
                     /gene="fusA"
                     /locus_tag="U2K71_000613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HEM7314722.1"
                     /translation="MTREFSLQNTRNIGIMAHIDAGKTTATERILYYTGKIHKIGETH
                     EGASQMDWMEQEQERGITITSAATTAQWKGHRINIIDTPGHVDFTVEVERSLRVLDGA
                     VTVLDAQSGVEPQTETVWRQATTYGVPRIVFANKMDKIGADFLYSVSTLHDRLQANAH
                     PIQLPIGAEDDFTGIIDLVTMKAEMYTNDLGTEIEETEIPEEYRELAEEWREKLVEAV
                     AETDEELTLKYLEGEEITEAELKEGIRRATVNVEFYPVLCGSAFKNKGVQLLLDAVLD
                     YLPSPLDIPAIKGIDPKTDEEVERPADDSAPFSALAFKVMTDPFVGRLTFFRVYSGVL
                     QSGSYVQNATKGKRERVGRILQMHANSRSEISEVYAGDIAAAVGLKDTTTGDTLCDEK
                     SLVILESMEFPEPVIQVAIEPKSKADQDKMGVALQKLSEEDPTFRAETNVETGETIIA
                     GMGELHLDIIVDRMRREFKVEANVGAPQVSYRETFRAGTQAEGKFVRQSGGKGQYGHV
                     WIEFTPNEEGAGFEFENAIVGGVVPREYIPAVETGLKDAMENGVLAGYPLVDIKAKLY
                     DGSYHDVDSNETAFRVAASMALRAAAKKANPVILEPIMAVEVVIPEDYLGDVMGHVTA
                     RRGRVEGMEARAGGQQVVRAMVPLAEMFGYATTLRSATQGRGTFTMTFDHYEDVPKSV
                     QEEIIKKNGGKAE"
     gene            43448..44635
                     /gene="tuf"
                     /locus_tag="U2K71_000614"
     CDS             43448..44635
                     /gene="tuf"
                     /locus_tag="U2K71_000614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEM7314723.1"
                     /translation="MAKEKFDRSKPHVNIGTIGHVDHGKTTLTAAITTVLSKKNGGQA
                     MAYDQIDGAPEERERGITISTAHVEYETDTRHYAHVDCPGHADYVKNMITGAAQMDGA
                     ILVVSAADGPMPQTREHILLSRQVGVPYIVVFLNKVDMVDDEELLELVEMEVRDLLTE
                     YEFPGDDVPVVAGSALKALEGDASYEEKILELMAAVDEYIPTPERDNDKPFMMPVEDV
                     FSITGRGTVATGRVERGQVRVGDEVEVVGIAEETSKTTVTGVEMFRKLLDYAEAGDNI
                     GALLRGVAREDIQRGQVLAKPGTITPHTKFSAEVYVLTKEEGGRHTPFFTNYRPQFYF
                     RTTDVTGVVELPEGTEMVMPGDNVTMEVELIHPIAIENGTKFSIREGGRTVGAGVVTE
                     IKA"
     gene            45042..45350
                     /gene="rpsJ"
                     /locus_tag="U2K71_000615"
     CDS             45042..45350
                     /gene="rpsJ"
                     /locus_tag="U2K71_000615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003046044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S10"
                     /protein_id="HEM7314724.1"
                     /translation="MAKQKIRIRLKAYEHRILDQSADKIVETAKRTGASVSGPIPLPT
                     ERSLYTIIRATHKYKDSREQFEMRTHKRLIDIVNPTPKTVDALMKLDLPSGVNIEIKL
                     "
     gene            45381..46010
                     /gene="rplC"
                     /locus_tag="U2K71_000616"
     CDS             45381..46010
                     /gene="rplC"
                     /locus_tag="U2K71_000616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HEM7314725.1"
                     /translation="MTKGILGKKVGMTQIFTESGELIPVTVVEATPNVVLQVKTMETD
                     GYEAIQVGYQDMREVLSNKPAKGHVAKANTAPKRFIREFKNVELGEYEVGKEITVDVF
                     QAGDIIDVTGTTKGKGFQGVIKRHGQSRGPMAHGSRYHRRPGSMGPVAPNRVFKNKRL
                     AGRMGGNRVTIQNLEVVRVDAERNVILIKGNVPGAKKSLITIKSAVKAK"
     gene            46038..46661
                     /gene="rplD"
                     /locus_tag="U2K71_000617"
     CDS             46038..46661
                     /gene="rplD"
                     /locus_tag="U2K71_000617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HEM7314726.1"
                     /translation="MPNVALFKQDGSQNGEITLNEEIFGIEPNESVVYDAIVMQRASL
                     RQGTHAVKNRSAVRGGGRKPWRQKGTGRARQGSIRSPQWRGGGVVFGPTPRSYSYKLP
                     KKVRRLAMKSVLSEKVAENNLVAIEGLNFDAPKTKEFKQVLANLSIDSKVLVVLEKGN
                     DFAALSARNLPNVSVVTSDNVSVLDVVSNTKVLATQTALTQIEEVLA"
     gene            46661..46951
                     /gene="rplW"
                     /locus_tag="U2K71_000618"
     CDS             46661..46951
                     /gene="rplW"
                     /locus_tag="U2K71_000618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HEM7314727.1"
                     /translation="MNLLDVIKRPVITEKSMLAMDDKKYTFEVDTRANKTLVKQAVEA
                     AFDVKVKNVNIVNVRAKFKRMGKYAGYTKKRRKAIVTLTEDSKEIQLFEAAE"
     gene            46990..47823
                     /gene="rplB"
                     /locus_tag="U2K71_000619"
     CDS             46990..47823
                     /gene="rplB"
                     /locus_tag="U2K71_000619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HEM7314728.1"
                     /translation="MAIKKYKPTSNGRRNMTGSDFAEITTSTPEKSLLQPLKNHAGRN
                     NNGRITVRHQGGGHKRQYRVIDFKRNKDNVVATVQTIEYDPNRSANIALVHYEDGVKA
                     YILAPKGLEVGMKLVSGPEADIKVGNALPLENIPVGTVIHNIEMKPGKGGQLIRSAGT
                     SAQVLGKEGKYVLIRLNSGEVRMILAACRATIGSVGNEQHELINIGKAGRSRWMRKRP
                     TVRGSVMNPNDHPHGGGEGKAPIGRKAPVSPWGQPALGYKTRNKKAKSDKLIVRRRKN
                     K"
     gene            47866..48144
                     /gene="rpsS"
                     /locus_tag="U2K71_000620"
     CDS             47866..48144
                     /gene="rpsS"
                     /locus_tag="U2K71_000620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000533765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HEM7314729.1"
                     /translation="MGRSLKKGPFVDEHLMKKVEAQAGVEKKKVIKTWSRRSTVFPSF
                     VGYTIAVYDGRKHVPVYIQEDMVGHKLGEFAPTRTYRGHAADDKKTKR"
     gene            48167..48514
                     /gene="rplV"
                     /locus_tag="U2K71_000621"
     CDS             48167..48514
                     /gene="rplV"
                     /locus_tag="U2K71_000621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003701308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HEM7314730.1"
                     /translation="MAEQITSAKATAKTVRTSPRKARLVIDLIRGKSVADAISILKFT
                     PNKSAGIIEKVLMSAVANAENNFDLDVENLVVSEAFVNEGPTMKRFRPRAKGSASPIN
                     KRTSHITVVVSEK"
     gene            48528..49184
                     /gene="rpsC"
                     /locus_tag="U2K71_000622"
     CDS             48528..49184
                     /gene="rpsC"
                     /locus_tag="U2K71_000622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000529931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HEM7314731.1"
                     /translation="MGQKVHPIGMRVGIIRDWDAKWYAEKEYAEFLHEDLRIRKFIST
                     KLADAAVSTVEIERATGRVNISIHTAKPGMVIGKGGSEVESLRKELNKLTGKRIHINI
                     VEIKKPDLDAKLVGEGIARQLENRVAFRRAQKQAIQRSMRAGAQGIKTQVSGRLNGAD
                     IARSEGYSEGTVPLHTLRADIDYAWEEADTTYGKLGVKVWIYRGEILPTKKNTEKGGK
                     "
     gene            49187..49621
                     /gene="rplP"
                     /locus_tag="U2K71_000623"
     CDS             49187..49621
                     /gene="rplP"
                     /locus_tag="U2K71_000623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HEM7314732.1"
                     /translation="MLVPKRVKHRREFRGKMRGEAKGGKEVAFGEYGLQAVDSHWITN
                     RQIEAARIAMTRYMKRGGKVWIKIFPHKSYTAKAIGVRMGSGKGAPEGWVAPVKRGKI
                     MFEIAGVPEEVAREALRLASHKLPMKTKIVKREEMGGESNEG"
     gene            49611..49799
                     /gene="rpmC"
                     /locus_tag="U2K71_000624"
     CDS             49611..49799
                     /gene="rpmC"
                     /locus_tag="U2K71_000624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HEM7314733.1"
                     /translation="MKVKEIRELTTAEMLDQEKQLKEELFNLRFQLATGQLENTARIK
                     EVRKSIARIKTVLREQAK"
     gene            49824..50090
                     /gene="rpsQ"
                     /locus_tag="U2K71_000625"
     CDS             49824..50090
                     /gene="rpsQ"
                     /locus_tag="U2K71_000625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HEM7314734.1"
                     /translation="MTEGRNQRKVYQGRVVSDKMDKTITVAVETKKNHPIYGKRMNYS
                     KKYKVHDENNTAKVGDIVRIMETRPLSATKRFRLLEVVEEAVII"
     gene            50148..50516
                     /gene="rplN"
                     /locus_tag="U2K71_000626"
     CDS             50148..50516
                     /gene="rplN"
                     /locus_tag="U2K71_000626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012727667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HEM7314735.1"
                     /translation="MIQAESRLKVADNSGAREILTIKVLGGSGRKTANIGDVIVATVK
                     QATPGGVVKKGDVVKAVIVRTKSGARRTDGSYIKFDENAAVIIRDDKSPRGTRIFGPV
                     ARELRENNFMKIVSLAPEVL"
     gene            50553..50861
                     /gene="rplX"
                     /locus_tag="U2K71_000627"
     CDS             50553..50861
                     /gene="rplX"
                     /locus_tag="U2K71_000627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HEM7314736.1"
                     /translation="MFVKKGDKVKVITGKDKNKEGVVLAAFPKKDKVIVEGVNVVKKH
                     QKPSQAAPQGGIVEMEAPIHVSNVMVVDSTGVAGRVGYKEVDGKKVRVSKKTGEVLDK
                     "
     gene            50889..51428
                     /gene="rplE"
                     /locus_tag="U2K71_000628"
     CDS             50889..51428
                     /gene="rplE"
                     /locus_tag="U2K71_000628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_466143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HEM7314737.1"
                     /translation="MNRLKEKYLKEVTPSLMEKFNYSSVMQTPKVEKIVINMGVGDAV
                     SNAKNLDKAVEELTLITGQKPMITKAKKSIAGFRLREGMPIGAKVTLRGERMYEFLDK
                     LVSVSLPRVRDFHGVSKKAFDGRGNYTLGIKEQLIFPEVDYDLVDKVRGMDIVIVTTA
                     NSDEESRELLTQLGMPFQK"
     gene            51447..51632
                     /locus_tag="U2K71_000629"
     CDS             51447..51632
                     /locus_tag="U2K71_000629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011245027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HEM7314738.1"
                     /translation="MAKKSMIAKNKRPAKFSTQAYTRCERCGRPHSVYRKFHLCRICF
                     RELAYKGQIPGVKKASW"
     gene            51668..52066
                     /gene="rpsH"
                     /locus_tag="U2K71_000630"
     CDS             51668..52066
                     /gene="rpsH"
                     /locus_tag="U2K71_000630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010742763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HEM7314739.1"
                     /translation="MVMTDPIADFLTRIRNANMVKHESLEVPASKIKRDIAEILKREG
                     FVRDVEYIEDDKQGVIRVFLKFGKNGERVITNLKRISKPGLRAYVKADEVPKVLNGLG
                     IAIISTSEGVVTDKEAREKNIGGEVIAYVW"
     gene            52098..52634
                     /gene="rplF"
                     /locus_tag="U2K71_000631"
     CDS             52098..52634
                     /gene="rplF"
                     /locus_tag="U2K71_000631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HEM7314740.1"
                     /translation="MSRIGNKVVVIPEGVTVTQDGNNVTVKGPKGELTRTFSADIKMN
                     IEGNEVTFTRPNDSKEMKTIHGTTRANFNNMVVGVSEGFQKALELIGVGYRAQLQGSK
                     LVLNVGYSHPVEITPPAGVTVEVPANTQVIVKGANKEEVGELAANIRGTRPPEPYKGK
                     GIRYVGEYVRRKEGKTGK"
     gene            52795..53151
                     /gene="rplR"
                     /locus_tag="U2K71_000632"
     CDS             52795..53151
                     /gene="rplR"
                     /locus_tag="U2K71_000632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000628816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HEM7314741.1"
                     /translation="MITKPDKNKTRQKRHRRVRNNISGTAERPRLNVFRSNKNIYAQI
                     IDDVAGVTLASASTLDKEISGGTKTEAAQAVGKLVAERAAEKGIKVVVFDRGGYLYHG
                     RVQALAEAARENGLEF"
     gene            53172..53672
                     /gene="rpsE"
                     /locus_tag="U2K71_000633"
     CDS             53172..53672
                     /gene="rpsE"
                     /locus_tag="U2K71_000633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HEM7314742.1"
                     /translation="MVYIDPKHLELEDRVVAINRVTKVVKGGRRLRFAALVVVGDKNG
                     HVGFGTGKAQEVPEAIRKAIEDAKKNLVEVPMVGSTIPHEVIGAFSGGRILMKPAVEG
                     SGVAAGGPVRAVLELAGVADITSKSLGSNTPINVVRATVEGLKQLKRAEEVAELRGKS
                     VEELIG"
     gene            53687..53866
                     /gene="rpmD"
                     /locus_tag="U2K71_000634"
     CDS             53687..53866
                     /gene="rpmD"
                     /locus_tag="U2K71_000634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HEM7314743.1"
                     /translation="MAELKVTLKRSIIGRPQNQRDTVKALGLTKINSSVVKPSNEAIK
                     GMINTVSHLVDVEEI"
     gene            53910..54350
                     /gene="rplO"
                     /locus_tag="U2K71_000635"
     CDS             53910..54350
                     /gene="rplO"
                     /locus_tag="U2K71_000635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005592004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HEM7314744.1"
                     /translation="MKLHELKPAEGSRQVRNRVGRGTSSGNGKTAGRGQKGQKARSGG
                     GVRLGFEGGQTPLFRRLPKRGFTNVNRKDYAVINLDVLNRFEDGAEVTPVALVEAGIV
                     KNEKAGIKVLANGELNKKLTVKAAKFSKAAQEAIEAAGGSIEVI"
     gene            54350..55645
                     /gene="secY"
                     /locus_tag="U2K71_000636"
     CDS             54350..55645
                     /gene="secY"
                     /locus_tag="U2K71_000636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HEM7314745.1"
                     /translation="MFRLLKDAFKVKDIRSKILFTVFVLFVFRLGAHITVPGVNAASL
                     QNLSSLPFFNMLNLVSGSAMQNFSIFSMGVSPYITASIVIQLLQMDIVPRFVEWSKQG
                     EVGRKKLNQATRYLTLVLAFIQSVGITAGFNQYAQLGFVNNPNVATYVTIGLILTAGT
                     MFVTWLGEQITEKGIGNGVSMIIFAGIISRLPESIREIYEDYFINIDPSDIWKSAIFV
                     AILLVAVLLIVTFVTFFQQAERKIPIQYTKRVAGAPTSSYLPLKVNAAGVIPVIFASS
                     FIATPNAVLQALSGRFGSENWYKVMMEIFNYNTAPGATIYTILIVAFTFFYAFVQVNP
                     EKLAENLQKQGSYIPSVRPGKGTEEYVSRLLMRLSTVGAVFLGLVALLPIIAQMVWDL
                     PQSIGLGGTSLLIVIGVALETAKQLEGLMLKRKYVGFIE"
     gene            55707..56354
                     /locus_tag="U2K71_000637"
     CDS             55707..56354
                     /locus_tag="U2K71_000637"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HEM7314746.1"
                     /translation="MNLILMGLPGAGKGTQAEKIIDAYGIPHISTGDMFRAAMQNETA
                     LGLEAKSYMDKGALVPDEVTNGIVKERLAEPDTEKGFLLDGFPRTLDQAKALDAMLKD
                     LNKKIDAVIDIHVGEEILVERLAGRFICSNCGATYHKIFNPTKVEDTCDRCGGHEFYQ
                     REDDKPETVKNRLAINIKNSEPILAYYKEQGLLNTIDGAREIDAVFADVQKIIEK"
     gene            56545..56763
                     /gene="infA"
                     /locus_tag="U2K71_000638"
     CDS             56545..56763
                     /gene="infA"
                     /locus_tag="U2K71_000638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-1"
                     /protein_id="HEM7314747.1"
                     /translation="MAKEDMIEVEGTVVETLPNAMFKVELENGHQVLATVSGKIRMHY
                     IRILPGDKVTVELSPYDLNRGRITYRFK"
     gene            56796..56912
                     /gene="rpmJ"
                     /locus_tag="U2K71_000639"
     CDS             56796..56912
                     /gene="rpmJ"
                     /locus_tag="U2K71_000639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HEM7314748.1"
                     /translation="MKVRPSVKPMCEHCKVIRRKGRVMVICPANPKHKQRQG"
     gene            56930..57295
                     /gene="rpsM"
                     /locus_tag="U2K71_000640"
     CDS             56930..57295
                     /gene="rpsM"
                     /locus_tag="U2K71_000640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008809894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HEM7314749.1"
                     /translation="MARIAGVDIPRDKRVVVSLTYIYGIGNTTAKKILADAGVSEDVR
                     VRELTNEQTDAIRAEVDKLKVEGDLRREVNLNIKRLMEIGSYRGIRHRRGLPVRGQNT
                     KNNARTRKGPARAIAGKKK"
     gene            57323..57712
                     /gene="rpsK"
                     /locus_tag="U2K71_000641"
     CDS             57323..57712
                     /gene="rpsK"
                     /locus_tag="U2K71_000641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HEM7314750.1"
                     /translation="MAAKKVNRKRRVKKNIEAGIAHIHSTFNNTIIMITDTHGNALAW
                     SSAGSLGFKGSKKSTPFAAQMAAETATKAAMEHGLKTVEVTVKGPGSGREAAIRSLQA
                     TGLEVTAIRDVTPVPHNGARPPKRRRV"
     gene            57792..58730
                     /locus_tag="U2K71_000642"
     CDS             57792..58730
                     /locus_tag="U2K71_000642"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010774253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HEM7314751.1"
                     /translation="MIEFEKPRIAKIDEEKDYGKFIVEPLERGYGTTLGNSLRRILLS
                     SLPGAAITNIQIDGVLHEFSTIKGVREDVTQIILNIKGLALKMYGQEEKTLEIDITGP
                     ATVTAGDIIVDSDVEILNKDMYICSVSEGATFHARLTVKPGRGYVQADENKKEDMPIG
                     VLPVDSIYTPVRRVNYQVENTRVGHREDFDKLTMEIWTDGSIEPLEAMSLSAKIMTEH
                     LDIFVNLTDEAKNAEIMVEKEETQKEKMLEMTIEELDLSVRSYNCLKRAGINTVQELT
                     NKSEPEMIKVRNLGRKSLEEVKAKLHDLGLGLRKED"
     gene            58758..59138
                     /gene="rplQ"
                     /locus_tag="U2K71_000643"
     CDS             58758..59138
                     /gene="rplQ"
                     /locus_tag="U2K71_000643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013773093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HEM7314752.1"
                     /translation="MSYRKLGRTSSQRKAMLRDLTTDLLINERIVTTEARAKEVRSTA
                     EKMITLGKRGDLHARRQAAAFVRNEVASVREENEDIVIESALQKLFNDIAPRYAERQG
                     GYTRILKTEPRRGDGAPMVVLELV"
     gene            59410..60249
                     /locus_tag="U2K71_000644"
     CDS             59410..60249
                     /locus_tag="U2K71_000644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM7314753.1"
                     /translation="MEPIIELEKINYKYQPDDLRPALKDVSFTIDKGEWIAIIGHNGS
                     GKSTLAKTINGLLLPESGIVKVGNQILDEENIWTIRQMVGMVFQNPDNQFVGSTVEDD
                     VAFGLENQGIPREEMLVRVKDALEKVRMAEFASREPARLSGGQKQRVAIAGVVALRPD
                     IIILDEATSMLDPEGREEVISTIKKIKEESQLTVISITHDIDEAANANRILVMRQGEL
                     VREGTPKEIFSAGPELIDLGLDLPFPEKLKSALKERGVDVPSEYMTEERMVDWLWTSV
                     LNK"
     gene            60225..61094
                     /locus_tag="U2K71_000645"
     CDS             60225..61094
                     /locus_tag="U2K71_000645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM7314754.1"
                     /translation="MDIRFEQVDFTYQPNTPFEQRALFDINMTIKENSYTALVGHTGS
                     GKSTLLQHLNALVKPTSGTVHIGERDIQPDTDNKNLKPIRKKVGIVFQFPEAQLFEET
                     VAKDIAFGPKNFGVSEEEALVLAKETLEQVGLDESYLERSPFELSGGQMRRVAIAGVL
                     AMRPEVLVLDEPTAGLDPQGRKEMMEMFWRLHKEQQITIVLVTHLMDDVANFADYVYV
                     LEKGRIVNSGEPQEVFQNIEWLKEKQLGVPTATEFAEELMAKGMNFATLPLTAEELAD
                     AIVHYAGGNAHDE"
     gene            61084..61881
                     /locus_tag="U2K71_000646"
     CDS             61084..61881
                     /locus_tag="U2K71_000646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     protein EcfT"
                     /protein_id="HEM7314755.1"
                     /translation="MMNKLIFGRYMPGDSLIHRLDPRAKLLASFYFIGIIFLANNWET
                     YLLLAFFTLLSVFLSKVDLKFFIRGIRPLIWLILFTVALQVLFTSGGETYWSWGVFHI
                     TEFGVMNGLFIFCRFVLIIFMSTLLTLTTPPLELSDAIEYLLRPLKAVRFPVHEISLM
                     LSIALRFVPTLMDETEKIMNAQRARGVDFGEGNLVQKMKAVIPLLIPLFVSSFNRAED
                     LATAMEARGYKGGEGRTKYRVLHWHNRDTMVIIAFVLLTVLLIFLRG"
     gene            62084..62830
                     /gene="truA"
                     /locus_tag="U2K71_000647"
     CDS             62084..62830
                     /gene="truA"
                     /locus_tag="U2K71_000647"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HEM7314756.1"
                     /translation="MVRYKAIIAYDGTNFNGFQKQPNGRTVQEEVEKTLQKMANGKEI
                     TVFGSGRTDAGVHAIGQVIHFDYPEERPLERMRFALDTQSPEDIAVRQVEIVSEDFHA
                     RYLVKEKTYQFRVDIGKPRSPFRRHYASYFPYPLDLEKIQRALPDLLGTHDFTSFCAS
                     GSSIEDKVRTIYEAKMEVNETKDELLFTFRGNGFLYKMIRILVGTLLKIGNGRLPEDS
                     IPDIIAKKDRNAAGPTAHPEGLYLYEVKYE"
     gene            63495..63732
                     /locus_tag="U2K71_000648"
                     /pseudo
     CDS             63495..63732
                     /locus_tag="U2K71_000648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299509.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(63876..64652)
                     /locus_tag="U2K71_000649"
     CDS             complement(63876..64652)
                     /locus_tag="U2K71_000649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM7314757.1"
                     /translation="MNIHHIRNATAILDYGGKKFLIDPMLAEKGTLPPFISKGPFPSP
                     RKDQMNPLVDLPMPVEDIINGIDAVIVTHLHLDHWDDIAKEVLPKEIKLFVQDENDAN
                     EIRLYGFKNVEVLREDTVFESIQLVKTKGKHGRGEILNYVGNVCGIVFKHSTEKTLYF
                     TGDTVWYEAVKQTIDQYNPEIIVANAGDNHFLVGESLIMGKDDLYELHKAIPDSTIIA
                     VHMEAVNHWGLSREELKSFNKEKGISEKVLVPEDGDNIFI"
     gene            64775..65182
                     /locus_tag="U2K71_000650"
     CDS             64775..65182
                     /locus_tag="U2K71_000650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HEM7314758.1"
                     /translation="MLDQTDINIIEELSKNSRITMKELGEKVHLTGPAVSARVTKLEE
                     SGVIENYTVKLNMEKLGFSIHAFLIIITQNLNHKPYLSFIKNHEKFLIRNYKISGGGC
                     YLLECRFTSKKSMNSFLEQLNEYANYKLSIVID"
BASE COUNT        20849 a        11661 c        13808 g        19209 t
ORIGIN      
        1 gcttctacgg aaataagccg aaatttcaca aaaatttgag aagcaatttt cgtaaattcc
       61 ttcttatttc ttgaagctga ccacttctgt cacaacctct tattttgatt agaacttgcg
      121 aaaataaacc ggttaaagtg tgtctagcgc aagagatttg ctataataga cgataagggt
      181 aaaatgtaac gagaaggggt tgaaaaatgt gtatcaaatt gtaacagatt cttgttgtga
      241 tctgccatac catgtattag caaaagaaaa agttgatttt ttatcaatga atctccaatt
      301 gaatgatcaa gagttagtag atgacctcgg aaagacattc aattatgacc gctacatgaa
      361 tcagctgaaa aatggtgcga tgcctacgac atcacaaatc aatgttggcc gatatttaga
      421 atttttccgt tcttatgcgg aaaaacagat tcctgtttta tatgtggctt tttccagtgg
      481 attaagcggc tcttacagta gtgcgcttca agcggttgaa ttgctgaagg aagaatatga
      541 agaccctgag atctatgttt ttgatacaaa agctgcaagt attggggaag ggcttttagt
      601 attggaagct gtaagattga aaaaagaagg taaaagcatc gaagaaaccc tagagtggct
      661 ggaaagtaat tatttgaaag tccattcatg ggtaacagta gacgatctga aatatctaga
      721 acgaggcggt aggatttcca aagcaagtgc tactcttggc agtctattga gtgtaaagcc
      781 tattattacg gtggataaag aaggaaggct gacgaatgtt gacaaagttc gaggaagaaa
      841 caagtcgatc cagacaatcg tcaatgagac ggtaaaaaca atcgttgcac cacttgatca
      901 aatcgtttat gtggcttacg ctggggatct agagagcgcg gagaaggcaa aacggctttt
      961 ggaagagcag ataaagatga aagatgtgaa gctttatccc ttaggaccaa cgatcgccag
     1021 tcacacaggc tatggctgta tcgcgatttt ttcgatggga atcagtcgat aaaagcagta
     1081 actcattctg taaaaaagcc cagaaattga ttgtaacaaa aacagcgact cttaaaagat
     1141 agatgagcat cctatctctt agaggtcgct gtttttttat tttttaagct aaataggcca
     1201 ttatgcccat aactcgttca attgtttttg aatatctgca ttttccaaaa attcatcata
     1261 tgtcgtatca gctcgatcga tcacaccttt atccgaaacg gcaatgatgc ggtttgccaa
     1321 tgtttggata aattgatggt catgtgacgc aaataagatt gagccagtaa atgccatcaa
     1381 gccgtcattc aatgctgtga ttgattctaa gtctaaatgg ttcgttggat catctaatac
     1441 aagtacgtta gctttcgaaa gcatcatttt tgaaagcatg caacggactt tctctccacc
     1501 agataaaaca ttcactggtt tcaatacttc ttcaccagaa aacaacattc ttcctaagaa
     1561 gcttcttaag aaggtgttat catcttcttc tttacttgca tattgacgca accagtctaa
     1621 aatcgttaga tcagtatcga attctttcgt tgtatccttt ggtaaataag aattacttgt
     1681 tgtcacaccc caacgtactg ttccagtatc tggcgtaatt tcacccatga taactttgaa
     1741 tagcgttgtt gttgttatat cattttctgc gacgaaagcg accttgtctt ctttgctcaa
     1801 attaaaagat atatcatcta aaattttctt tccatcaatc gttacactta cattttctac
     1861 ctgcaataag tcgtttccaa tctcacgttc aggtttgaat ccaacgaatg gataacgtct
     1921 agaagaaggt tggatatcgt ctaaagtaat tttttcaagc atttttttac gtgaagttgc
     1981 ttgttttgat tttgatgcat tcgcactaaa gcgggcaata aattcttgga gttctttgat
     2041 ttgttcttct tttttggcat ttgcgtttgc ttgaagttta gccgctagtt gactagattc
     2101 caaccaaaaa tcataatttc ctacataaag tttgatttta ccaaaatcta gatccgccat
     2161 atgtgtacat actttgttca aaaagtgacg gtcatgggat acaacgatca cagtattttc
     2221 aaaattgatc aaaaattctt ccagccaatt gatcgattgg atatccagac cgtttgttgg
     2281 ttcgtctagc aaaagaacat caggtttacc aaatagtgtt tgtgctaata acacttttac
     2341 cttttgtcca ccagttaatt cactcatttg ctgatggtgc aattcttcag ggatgttcag
     2401 accttgcagt aaaacagctg cttctggttc agcttcccat ccattaagct cagcaaattc
     2461 tccttctaat tcagctgctc gaataccatc ttcgtcagtg aaatcctctt tcatgtaaat
     2521 cgcatctttt tctttcagca cttcatacag acgcttgtgt cccatgatga ctgtttcaag
     2581 tacagtatag tcttcatagt cgtaatggtt ctgtttcaat acggccatcc gttcatctgg
     2641 tcccatagta acggaacctg tggaaggctg cagatcccct gataacactt taaggaacgt
     2701 tgatttacct gcaccatttg caccaatcaa gccatagcag tttccggggg taaatttaat
     2761 attcacatca tcaaatagtt tgcggtctga aaagtgcaaa ctcacatcat ttacagtaat
     2821 caattttttt cctcactttt atcattatta gttttgacaa gtcttataga attatatagg
     2881 ggaaaggtaa aagattcaag gcaatcgaac attctttgtg tttcacgtga aacagaatga
     2941 aaaaaataaa aataaaagcg gagcgaaaat agctactata ggatttatta gtttgcaaca
     3001 gcataatttt tgacgttatt tataagaggg agtactataa aactgtaaaa gaaatgaaag
     3061 aaggattgaa aatggatgaa aaaagaacca tcgactgggg ctcattgatt ttgggaattt
     3121 tatttgtact tgtctcattg ttatcatttc aagaccctgt cggaaattta gttgcaattg
     3181 tagtagtctt tgcgattttt gcctttatta aagggatatt tgaactgttt gtacggaaca
     3241 gattaaaaga gctaacagga tataaaggaa agatgccctt aataatcggg atcatcgata
     3301 ttctagttgg cgtctttttc ttattcaata ttggtgcagg agtcgcagca ttgccttttg
     3361 tttttgcagt atggtttatt gctgattcag ttttagcgtt atttactgct gacttggcaa
     3421 gaggagtaag tgaaggatat tattggttta cgatcatcgt caatatttta ggaattttat
     3481 taggaatttt cttattgttt aatccaattt cttctgcgtt gacattaagc tttttagttg
     3541 gattctattt tatgttattt ggtatcacac atatcgtcta tgcatttaga taagtaaaat
     3601 caaacaaata aaaaagccgg cttccgatta cagttttact gttgtaattg gaagccggct
     3661 tttttatcat ggacaaatat gcacgaggtg atttttaggt gtcgagacaa atttatgcaa
     3721 aaatgtgaga agctatatac acaaatttta tcttaatact cgaagcatag cgcagttgtc
     3781 acaacctctt ttcatataat tcattcaata ctggcaaatc atggacttca tatttttctt
     3841 tgaatgcttg gatttcttct tcagaatatt ttcttggatc taaatgcaga tcttctactg
     3901 gcaatgggcg cttgttattg atcttcacat cgattactac aggacgatct gattttgcag
     3961 ctgcatcaaa ggcaaatcga agttgcgagt attctgtgac tgtaaagcta tttgccccga
     4021 gcgcttctcc aactttaccg aaatctgcat cttctaaaaa tacaccaaat ttcttttgtt
     4081 ccgtatcttc ttgttctgct tcgatgaaac caaaagaatc attcgaaaag acaacattaa
     4141 taattggaag ctgatatttg acttgtgtaa tgatatcttg catgaccata gcaaacccgc
     4201 catcaccact taaggtaaat acttgttttt ctgggtagct tagttgtgcg gcaattcctc
     4261 ctggcacacc attacccata gttgcgaacc atccagatgt cgtatgtttt tgtttaccgt
     4321 tcatattcaa taaacgaata gaatggatag tcgtgttacc gacatctgtg acgaaaatcg
     4381 catcgtcttc agcaatacga ttgatttctt tatagacagc ctctggacga atcggttctt
     4441 cctctcgatc ttcaaaagat ttcaaccaat ttacccattt ttctttattt tcttgatttg
     4501 ctaggtacca gctatcagca gggcgagcat cccctaactc gacaagtctt tgcaaagctg
     4561 tgtttgcatc accgaggatc gacaaagatg tttcatggcg tcgtccaaat tttgacgcat
     4621 cgatatcgat ttggataaat tcagcagttg gattaaaaaa tgcacgtcca aatgggaagt
     4681 cgctaccgac aaataagatc agatcggctt ctgccaatgc ttcatttccg ggtttagtag
     4741 cgacacgtcc agcaaaacca agaaagtttt cataatcatc aggaataatt ccttttgcta
     4801 gaacggaagc gacaacagga actgaaaaat gttctgagaa ttctttgatt tgcggaaatc
     4861 cgtttcgcgt tccttgacca acataaagaa ctggtttttt tgctttttca agataaggca
     4921 aagcagctag caaatctttt tcttctggta atatcacact tgttttgtga gtgtgagcgg
     4981 tggagaaagg ttgttcgtca atttcttcaa aaccaagatc aacagggata gtcacgatag
     5041 ccacgccttt atgctcatag gcggctttaa tggcttcatc tactacatgc ggaagacttt
     5101 gtggtgtcat gactgtacga ttgtatacgc taacatctgc gaaaatagga ttttcattta
     5161 gttcctggaa agagttatag ttcatggatg tagaagcaac ttgtcctaag aacgctacta
     5221 gaggcacatg gtccatctgt gcatcataca aaccattgat caaatgtgtc gcaccaggtc
     5281 ctgcagagcc gaagacagcg ccgactttcc ccgttaattt tgcatctgca gcggctgcga
     5341 gtgcgccgac ttcttcatga cgaacttgaa tgtagttgat ttcggttttt tcttgatata
     5401 gcgcatccat aattgaattg aaagatcctc cgggaatgcc gtaaatatgg tcgatacccc
     5461 agctttcaag aacttttacc attgcgattc ctgcattgat ttttgacatg gtgtttcact
     5521 cctttagcaa tgatgataca taaatttttt gtatctagag tatagaactt tttatcgaaa
     5581 atcccaacta attcgtcttt tcacaaaaat gtagtcatgt ttttgggaat atgctagagt
     5641 agtacataga tagattcaag agaggggaat ggcatggttg aatggctatt gagacttgaa
     5701 ccttggcagc aagcacttgc tgggacagct tttacttatt ttatgacagc tcttggtgcc
     5761 gggttggttt ttttctttaa agaaatcaaa aaagaagtgc taaacttgat gttaggattt
     5821 gcttctggtg tcatgattgc tgcaagtttt tggtctttgt tagatccggc tattacaaaa
     5881 gcagaagaaa atggagatat tgcttggcta gttgtaagta ttgggtttgg tttaggcggt
     5941 gtgtttttat atatggcaga taaaacactg ccccatatgc attttggacc acaacatgaa
     6001 gcagaaggat tgcctaccca tttaaaacgg acgatcttgc ttgttttttc tattacattg
     6061 cataacattc ctgaaggttt agccgtgggg gtagcattcg gtgcagcagc aactgcggat
     6121 aatccgacag ccgctatatt agccgctgtt tcggtggcgc taggtattgg tatccaaaac
     6181 ttcccagaag gagcagctgt atcaatcccg ttgcgacaag aaggtctgag tcgtaaaaag
     6241 gcctttgttt acggacaagc ttctggaatc gttgaaccaa ttgctggagt gattggagcg
     6301 ctactagtaa cgaaagtgga gctgctgctt ccttatgcac ttgcttttgc cgcaggagca
     6361 atgatttatg tcgtggtgga agaattgatc cctgaagcac agcagacatt gtccagcaaa
     6421 catcattttg cagtttttgg cgtcatgagt ggatttatca ttatgatgat tttggatgtg
     6481 gcattaggat aaataaaaaa aatatatatg atatatcaaa tagggccgcg aacttatatc
     6541 gcgaccctat ttttttagta agttatatca taatgaagtg aatggtatca tacattaata
     6601 atagtttttt ctatgtgtat ttcctttgat ccagcgaaca agccagatga taagtgcgat
     6661 tgggattaga actttgaccg caaaccataa aagcccgctt aggacactga caaaaagact
     6721 gatgacgaag ctagctgcaa ttagcactaa aagaaccatg aagatattta agaacatatt
     6781 tttttgacgc atacaaaacc ctcctcttgt tcattcttta ctatagaaaa agtataacag
     6841 ggattgttta aaaacacctc caactgaaga ctgattttta cttcatctac ttaaatgatc
     6901 aaacaattgc tttttctctg gtttatcatc taaaatagag cggaaaagga ggagaatatc
     6961 atgcctaaaa atcgtcttga agcatttaca gatgctgtaa tcgcgattat catgactatt
     7021 ttagttttag aacttcatga acctaacgga gacacgtggg aggcgctcgc tgcattaggt
     7081 taccgatttt ttatctatat tgtcagtttt atcattttag cgatttattg gaacaatcat
     7141 catcatttat ttcaagttgt acataagata aatggacggg tcttatgggc gaacagtttc
     7201 tttgtttttg cgctttcgct ttttccgttt gcaacttcat ggatcagtga gtacatcaat
     7261 agcctggctc cagaaatgac ttatggtttt gttaccttga ccgctaatat tgcgtattat
     7321 atcttggcaa aagaacttgt caaagcaaat aaaggccacg agaaagcatt gaaggcgatg
     7381 ttgaataatt acaagaaatc gtatctatcg attggtttga atgtgttagg aattgtctta
     7441 gggtatttag tagcgccagt agccgtgctg atcgcaaaca tcgtgattct agctgcttgg
     7501 atcatcccaa gtcgtaccat cgaatcacaa tatcggtaaa atgatccgtg tttaaaattt
     7561 aagagaaaaa tatagaaaaa ataaataaac gatcaaatat ccgagtgata ctagtattca
     7621 aagagggatg cagatggatc aaaaaagaat tcatgtagga ctgattacta gttttgtcac
     7681 gatgcttatt tatttggatc aaggtcattt gacttggaca gtagttggtg tcgtaggatt
     7741 tttgacatta ttcacagcaa taaaaaaata aacatcttta tgtaatctga tgttctatct
     7801 atttaagaaa agcatgttta cagcagtatg attctgttgt aaacgtgttt ttatttgtgg
     7861 aaaatttaat ttttagaaaa gtgtatcgaa aaatagatag ttagtttttc cacagtggat
     7921 aacttcgtaa gagataaaaa taaaaaatta gtcaaagaag ggcgtataga tttcttttga
     7981 taggagatat acaacagaaa tgaataaata ttaacgtgtt ttcaaacagt ataaaacggt
     8041 gtggtttttg gaataaaaaa ataacatatc tcattttaca cttttgttat tttatactta
     8101 aaaaataacg acacagaaaa taccgtgtct cagatttctc tactacttct aaaaactact
     8161 gtttttttcc tttgaaatgc cttcaaaaag taggatcagg cagaatatac tggcagaatc
     8221 aaggagaagg gagcctaaat aaggagaaaa accatagaaa atataaagta attacttata
     8281 aaacaagctt tttatagaaa aataatatac ctgagtaggg tataaattaa gacggaattc
     8341 ctttctagat tgagatattg actaggaaga atgttaagat tttaagcctt taagctaaaa
     8401 aaaaaccctt tttgttaaat aaggggaata tagcctgtca gtttcaaact gcaagtcgtc
     8461 ctgctgcaat aatatagtag attagagatg aaaatagatt tttttaaacc aatgactgaa
     8521 taaaaagacc gtcatagata cacattggaa tgtgtctatt acggtctttt tttacgaacg
     8581 attttattga agacggacag ttcgggtcaa aaataaaaaa gctacgatca gaaataagaa
     8641 taaaatgtat cccaaaataa tggctagacg gtctaactta tttgtcgata gatgtttgtt
     8701 tagaagttga taggcccata aacctatcag tcctcctgtt gtattagtta tcaaatcagt
     8761 gatgtccgct acgccaatac cgaataggaa ttgaatcaat tctgccaaga agcttacaag
     8821 gaaaataatc cctaattttt tagaaaaaga aagtctcttg aaattgacat tcaaaagcac
     8881 cccaaaagga aaaaagacaa tcaagttata agtggtctca cgtaataccg tcgtattttc
     8941 aaaagaaaaa gtagaaaaag ggacgagatt gacattgctg taagaataag tgagaaattg
     9001 tggaagatta aatgatagct tgaataaaat caaccataat agaaagaata aataaatccc
     9061 taataaaaat tttgatacat accgcataga catctcctcc atttaaaaag tataccatcg
     9121 aaaaccgctg aaaaatcata aaaaagtatg aagtttaagg accaacgtag ttatcgtacg
     9181 aaaattagaa gctttttttt gttgggaatc cggtttcttt aacaaagcta agatagcgag
     9241 ttgcttggaa aagttgctat cttttttctg tcaaaacgac tcggaatgtt cgttttttgt
     9301 tctaatttct ccaagttgtt tactttttga tactaagttt atgaaaaatc aagaaatagt
     9361 tgttatacta ggtaatgctg acaaacggga ttggcatttc atcttggttc agcttttttt
     9421 ccaattacta ggaaggaggg atagattgtt caagacttat tttcgtttca ataaaataca
     9481 agataatcct tttcgtatca tgaatctgat gctacttatc tttattgtat tatggttagg
     9541 ttacataaat catgattttt tgatttcaag tttttcatca ttagggatct ttactttttt
     9601 ctattatcaa aacataccga tgaaatattt gctgaaacgt atgtttctga tcggttgcgg
     9661 actgttggtt gctttcgttt tggggatctt atcgacttat gttttatggc ttgaaccatt
     9721 tgccgtagct ttggttgctt tttccagcag attcgtgttg cgattattcc atatttctaa
     9781 accaggtggc ttgttttttg cgatgctttc tgccatggga acgagcatgc agctgccaat
     9841 cgctcaatta ccgatcgtct cactttattt ttttatgggt gtcgtttttg ccctgattgc
     9901 ggcagtgatt acaaaattac tggattcacg gcctgagcag acaattgaaa aagcaacttt
     9961 aaaagaaaga tttcacgaag agcctttagt gatcattgat tctgttttct acagcgctgc
    10021 cttatttctt tccgtttatg tcagtcacgg cttgaatctg cataatcctt attggttgac
    10081 cttgtcatgt gcatcgattt tgctagcaga aaacttggat gcgatgaagc atcgacaagt
    10141 ccaatatctg attggatcga tgggtgggtt atgtgtgtcg gcattcttgt catttgtgcc
    10201 gtttacgcag ttgcagacca tttttctgat cacttttcta tatggaatcg cacaatttct
    10261 agttgctaga aattatgcag ttgctaatat ttttcttaat ccgatggcgt tgatgctatc
    10321 tacacttatt cgtggagcat acttgatttc tttgatcgaa tatcgttttt tagggatcgt
    10381 cataggaagt ttcatcggtt taggtgttgc gtgggtaatg acggttggtt tgcaacatta
    10441 tttaacggtt gttcgtcaaa aattagaata gaaggacaaa tatatgtgcc aaaagaggtt
    10501 atgacaagat ttttgtcaca acctctaatt caaggtgttc aaaagccaga ttctcagcat
    10561 taagtcaaat tcacacaaaa atgtggaaag gaactttcaa aaaaatttta tatttattct
    10621 atcaaaaaat tttgtttatg atggtaactg aataaacttt cctgagatgg tcaatgcttt
    10681 ccttgaaagt cttacagata ttctttcttc atagaaagtt taagttttat agttagtgta
    10741 caataaaagt atgtttatac catatggtgt ccggataatc agtcatttaa acaatgaggt
    10801 tcaaagatat gaaaagggga aatttgatga agaaaataag caatgaaatc caaattctgg
    10861 cagaaacttt aactcttccc aaagtcgtta tagagcaaat gattgtacaa gcaaaaaaca
    10921 tccttgatga ggtgtttgag caatatagtc atgaactaat ggatcctaat aaagcaaagg
    10981 cgactgcaaa gaaaatagac aaagtctatg aagaggaacc ttttgcagca ttagctattt
    11041 atctatatgc agcttctcaa agttggaaaa aaatttatca accattgaag attccaagaa
    11101 ctatctattt ggcaacaatg aatgctttta ctagatttat tcatgaacat ttccaagtaa
    11161 caggaagcta tcgctttgac cgtggttttt ggatttggca ttacactagt ggcttgatct
    11221 ttcggattgg tgaattggag tacgaaatga catattttcc gaaagggtac acggatctaa
    11281 gattagaagg aaagcagtgc ctttctcttc atattccttc cgatgcagat ttatctcaga
    11341 ataagataag agatagttac gaaagtgcca ttcgtttttt tgaactttat tttcctgatt
    11401 accattacca agtgatgtac acagatactt ggttactatc acccaatctt actaaatggt
    11461 tgaaaaaaga gtcaaaaatc tgtctttttg cagccgatta tcgattatta tcagttgatg
    11521 aacaggacga tagtggagtc ccttggatct ttggacgagt agatgcccaa atacatgatt
    11581 atccagaaag tacgagttta cagcgacaag caaaagagca attgcttgca ggcgagcaca
    11641 ttggctctgg cttaggtatt cttacttcat aagtttagta cttcgaaata gtttaaaaga
    11701 gcgtgagaca aaagtaaaga acacttttgt ctcacgctct aaacacgtat aaacggcgga
    11761 agcagaagca actccttcgg aaataagctg aaattcacaa aaatttgaaa agcaattttc
    11821 gtgaatttct tcttatttct tggagttaaa cgcttctgtc ccagcctctt ttttagtgtt
    11881 ctatcgaaca cacagtacgt tcaataggta aactagctat aacaatagat attaaagtta
    11941 acggacaaga gggccttttt taggtagcta aaacgtaata atgaaaattt atttccagag
    12001 ataggagtca atcagctagt ctgatcgact gttttaacaa gttgactttc attttaactt
    12061 ttgataccat ttaaaacaca aatcgtaatt gtttcgattt gtgtttttta gcatgactac
    12121 aatcaaagaa gaaagtgggt cgtccatatg gaaaaaacag atatctcaag tgcataccgc
    12181 cgcttgaaaa gcccaaatat caaaacacga aaacgtgcgc tgaagatcat caaagatgtg
    12241 aaacggaata gtgggaaaag gtaagaagaa ggaaacttaa ttaaaaaaat agccgaaaaa
    12301 cgtttttcgg ctattttcaa taatcggttt ttgtgtaagg tttccgaaag aactgctgtt
    12361 gattttccat ttttaacata tgataaattg gtagaatctt tcttttcaaa ttgataccat
    12421 tccctaattc cagttatata gttaactaca gaaccacaaa gcacgtacgt ttataaaagt
    12481 attatctgta gttatttttc aattcccttt caacctctta ttttgaccag tagtattgat
    12541 cactttacct tttaacacct ttgttcgttt ttgaaacatt tttagtcaag aatggaaacg
    12601 taactacata atcaaaggtt ttgcgaaata tttcatgaaa tatttcatgt agaagcgagc
    12661 aatacggttg caaataacca ttatcgttta ataagcagtt acgaaagctc ccaatatctc
    12721 taacgttcag ctcactccat ttcttttctt caaaaaaatc agtgattcgt atctctttct
    12781 atttgtcgaa aaagttaact ttaaaaagta acgccgaaaa aatatgcaaa aaaataaaaa
    12841 agcagttact attgctagaa taacatcaag gaataataag ttttggtttt cagataatag
    12901 tcgataagtt gtgtattgga attttagata cagtaataaa gtatttttca tgaaattttg
    12961 tgagaatcac tattacaagt atctttaata aaattaatgg ttaaggagga acttaaagta
    13021 gaatgtaaat gaatagttgt agtcagtcga ttgtgatact agactcaaag ttgcaggaat
    13081 aagtttttca atttgtgatt ggaagataaa aaatgaaaaa gaagggcttt tttttatact
    13141 ttgttgattg tagtgtttat tttccctctg tttagccaag taacattttc tatgactcaa
    13201 ccagtttcaa gtgaggaaca tgataacctt tttcgtgcta cagaagagtt taagaaatat
    13261 caggaaaata ttgttccgga gactccatta gctagtgagc caattactga tattgatgga
    13321 aaccaaatag gatggtatat acagtacgaa gttatggtgg taaataatga ctcaatagta
    13381 aaaaatacag taggcttaga gctaacaccg attgaaaagg tgagttcatt gttaacgtat
    13441 atttatactg atgaaagtga cgagactgag atcattttaa cggattactc aaaaattgct
    13501 actgataaaa agtatatatt gtggatgtag acactagtga aatactttat gatatagaaa
    13561 ttgatgaaaa tttactagat gtagcacaag aaatcgatca tcaaaagcag gaagctattc
    13621 gatatgctgg tagaaaaggg aaaagaaaat atttattacg tgaatgtagc aaaagttaga
    13681 gaagatgaaa atgagtggaa agaattcaag agtcgctact caattaactc gactccgaca
    13741 tttacagttt acagagaagg atcgatagaa aaaactgttt tttggactaa agaaagtgga
    13801 atgtctttag ctgaagtaga agagtttttg gattatgtta gtatgcaaca gtaagggagt
    13861 tcttattatg aaaaattttg acacatatta tgttattgtt ttttcgatat ctttgctgtt
    13921 aatattacta actttaatgc agtataaaga gtatccagag tattttattt taacttttgt
    13981 atatttagta agtagctaca cacttctata taaattcaga agttcttcta gagtttctaa
    14041 agtttgtttc ttaattagct tattgattct ataatatttt tgatttatat aggaaattaa
    14101 aaatattata gaatcaacac tcagagaacg ggttggtttt taatagattt aataagtact
    14161 gataatgaaa tttctcctgg ttgggaaaac gaagttggtt accctgtaga agaatttttt
    14221 caagattact ttgcggagta tgccgatagg ataatgagag attacgtaac agataaaact
    14281 caagaagtca tcaatcaatc ccagtcatta aatgaattat ctgataaggc gaaagaagaa
    14341 gcagttgcca ttatttcttc agggattagt gactatattg atatgagtgg tgaaatcaat
    14401 tatggcgata aagacaaaat tgtacgcatg ataaaagctt ttatcaaaac ttttgatgtg
    14461 aaggcagatc ttaagtttaa tgatacttac ctagtaggaa aattaattaa tatccttggg
    14521 acagagaaag atacggaaac gtttatttcc tctttgtcta tgttgtttac agataagcat
    14581 ttttcaactc agcttggtgg agaggaacta gtggaaatct tttaaccatt acgcccagca
    14641 ttaataaaag tatatcaaaa aagtactcct gatgattttt aagattggtt tgagaaataa
    14701 tgtgaaaaca ctatttttaa tgttaattat agatagagat gaacgcgtca tatggttaaa
    14761 agaatgaaaa agtcaaagat acactgctat atttcactaa ggatatagca gtgtatcttt
    14821 catgtcgttt cactggcagt tttttataaa aggaggtgtg catttcagtt gcttttcttt
    14881 ggtagttagt tgaatcgttt aagaattaac ggatgtggca gacaggtgct ctgtccaaaa
    14941 accagtaaaa tctttgatgg ttccatcttt atttagctgt gcctgttcaa atactgtttt
    15001 gtttttaccg aatggatcaa ttgcaataat tgttccgatc cccgcacgtt tggcagcaag
    15061 taatcctgag taagcatctt cgataacaag acaatcagcc ggagctaagc tcagtttgtc
    15121 tgccgcttta agaaaaatat ccggttgcgg tttcccaggg aaagagccgt cgtcatagac
    15181 aatcttgtcc cagtcaaacc agcgggctaa atcgaaaata tcaaaataaa aagcaacatt
    15241 ctctttgaca gtagccgtgg caatcgttaa tggaatctgt tggtcagtca gctggtcaag
    15301 agtcgcttca agcccttttg tcaactgaag ctcgttttca ttttgcaagc aaagcatacg
    15361 ataatagctt tctttttctt ctgacaatac ggcgatctca gaggcggtca aatgctctga
    15421 aatgaaatgt ttcaaaatct ccgtattcgt ccgaccatga atatttttta aaatatcctc
    15481 gtctgataaa ccatcttttg tatgatcatg tatcatatgc agccaagcag cttcatgcag
    15541 atgagagtcc aaaaacattg ttccgttgaa atcaaatata atccctttca tagttacgct
    15601 cctatagtgg tattttcggt tgaatcagca atttgcagat cgacatattg tgcatagtgc
    15661 tcttgaacct cccgcgagat ctggcgatcc gtaatgagca gatcgaaatc atgcaaatta
    15721 taaaacacat aaaaatcatc tcgattgaat tttttattat caacgagtag atatttctta
    15781 cgggaattat ttagagcgat ctgctgtgct tctccttctt cggtactgta agtagagatc
    15841 gcttcgttgt gaacgccatt tgaactgata aatgcttttg taaactttag tcttcttaaa
    15901 ttgtcgttgg tgatcggtcc gacaaatgcg ccggtatttt tccggaaatc accgccaatc
    15961 agtagaattt ctggtccatc tgcttgagca agaatatcaa aaactggata attatttgta
    16021 atgatccgaa ttttcttgtt cagtaaatgc gtcgccaata gttcgattgt tgtacctggt
    16081 cctaagaaaa tcgtttctcc ctcgtgaatc aaggaggctg ctgtggcagc aatttctttt
    16141 ttttcttgaa tctgcagagt ggctttttca gtgtgagaaa gttcttgatc taaagtgtag
    16201 gaaagacttt gagcccctcc atggacacgc agcagcttgc ccgatttttc taattcatct
    16261 aaatctctgc gaacggtcat atcagaaaca tctaattgat tgatgatttc attaacggta
    16321 aggattcctt ttttattgac catttctacg atagtaagca atctttcttt tttcaacatt
    16381 gtcttcacct ctaatttaag gataatctat catataaacg gacaacagtc aatctaattc
    16441 gaacaaaagt aaacatttag ggtggttttt atgtttgtta caatagattt tattgctgtt
    16501 ttctggtttt tatgattatt tgtgtttgtt tttgttgaca aatgttttgg atgcgtttac
    16561 aatgcaatca gttagaaaga gcgaaaactg attatagatt tgaggagaga tgtacatgct
    16621 taaattacca gaagatttta tttttggcgg agccacagca gcctatcagg ttgaaggagc
    16681 cactaaggaa ggtggcaaag gcgctgttgc ttgggatgac tttttagagg aacaagggcg
    16741 attcagtccc gatccggcaa gtgattttta tcatcaatat gcaaaggaca ttgaattgtg
    16801 tgaacgtttt ggtgtaaatg gtctaagatt atccattgct tggagccgca tttttcctga
    16861 tggagctggg aaaccaaatc cagaaggaat cgctttttat catcgtgtgt tcgaagaatg
    16921 taagaaaaga aacgtaacgc cgtttgtgac gttgcatcat tttgataccc ctaaacgttt
    16981 gtttgaccat ggagattttt tgaatcgaga aacaatagaa gcatttgttt cctatgccat
    17041 tttttgtttt catgaattca aagaagtcaa ggtgtggagt acctttaatg aaatctatcc
    17101 agttgcaacc aatcaatatt tgttaggtgt tttcccacca ggaatcaaat atgattttac
    17161 aaagatcgtc gcctgtcttc ataatatgat ggttgctcat gcccgtgtag tgaactattt
    17221 caaagaaaac gaacttccgg gtgaaattgg tgtagttcac tcattagaga cgaaatatgc
    17281 ggcaacagac gcgccggaag ataagcatgc tgccttttta gacgatgcat tgtcgatccg
    17341 tttcttatta gatgctacct atttaggcta ttattcaact gaaaccttga ctgctcttga
    17401 tgagatatgt gaagcaaatc aggccagcta tcattttcct gaagaagatt tcgttgaatt
    17461 gaaaaaagca tccacgcgta atgactactt agggatcaat cactaccaat gccattttgt
    17521 caaagcttac gatggagaaa atgcaatcca tcataacggt acgggtgaaa aagggacctc
    17581 tgtttataaa gtgaagggaa tcggagaacg tatttataaa gaaggaattc ctcgaactga
    17641 ttgggactgg ctgatttatc cagaaggtct atatgacttg ttgctgcgaa ttaaaagcga
    17701 ttacccacat tacaacaaaa tctatatcac tgagaacggg atggggtata aagatcagtt
    17761 tgaagatggc attattatgg atcagccgag aatcgactat ttgagagtct atttggaatc
    17821 cttaagcaag gcgataacag ccggggtaaa tgtgaagggg tattttttgt ggtcgcttat
    17881 ggatcttttc tcatggacaa atgggtataa caaaaggtat ggcttgtttt atgttgattt
    17941 tgaaacccag aagcgttacc caaaagaatc agcttactgg tacaagttgg tcagtgaaac
    18001 caaaacgatt atttaataag tgataatgga ggaagaaaag atgaataaag aggaattaca
    18061 aatgcttggt tttgagatcg ttgcatattc aggcgacgct cgcagtacat tattgaaatt
    18121 attgaaagaa gtaagacaag gaaactttga acatgttgat tcggcgttga aagaagccga
    18181 cgaaaattta acccttgcac ataattcgca aacaaaaatt ttagcggaag aagcatctgg
    18241 gaaagagatg gagatgggat tcatttttat ccatgggcag gatcatttaa tgaccacttt
    18301 attgctgaga gatctgattc aagactttat tgtactgtac agacagaatc aaggatagga
    18361 gaagaacgct atggatggaa tcattaaaca aatcgaaaaa gggaaaccat tttttgaaaa
    18421 aatctcacgt aatagttatt taggagccgt tcgtgacgga tttttaacag cgatgccagc
    18481 tattttattt tcaagcattt tcattttgat tgctgcggtt cctgagattt ttggttacca
    18541 atggcctgaa tctgtctcca catggctatg gaaagtctat aattattcga tgggtgttgt
    18601 agggttactc gtttcagcaa ccactgctcg ctgcttggct gattcgatga atcgtcgctt
    18661 accgaaaaac aaagtaatca acaccacctc tgtgatgctg gcctctgtgg taagtttcct
    18721 gttgttaagc gtctctccga ttgaaggtgg attttcaacg gaatatatgg ggacaaaggg
    18781 actactggct tcctttgtcg ctgcattttt aacagttaat ttatataagt tttgtgtgct
    18841 gaaagatatt accattaaaa tgcctaaaga agttcctgga acgatttcgc aaaccttccg
    18901 agatattttt cctttctctt tttcagtatt tgcggccgtt ttaattgatt taggcattcg
    18961 acaaatcttc caaatctcat ttgcagaagc aatcattacg gtgctgcagc cgctatttac
    19021 agcagctgac gggtacttag gaatcgctat tatctgggga gcgatggcgc tgttttggtt
    19081 tgtcggtgtt catggaccgt cgatcgtgga gccggcaatt gcggcaatta tctatgccaa
    19141 tgtggaaacc aatctgcaat tgttcaattc cggtgaacaa gcttctaaag tactgacggt
    19201 gggtttaggg aattttgtag gaactatggg aggaactggg gcaacattgg ttgttccttt
    19261 tatcttccta ctctttgcta agtctaagca gttgaaggca gttgggaaag cttcctttat
    19321 tccagtaagt ttcgctgtca acgaaccatt gctatttgcg gcacccatga ttttgaaccc
    19381 ttattttttt attccatttt tgttaacacc tgttgtaaac gtttgcctgt tcaagttttt
    19441 tgtcgatgta ttgggcatga acagctttat gtacgttttg ccttgggcaa ctccagcacc
    19501 gatcggtttg gttctaggaa cgggcatggg tatcttagct tttgttctag cagcggttct
    19561 aatcgtcgtt gacttcctga tctatctacc attttgcaaa gcatacgatg ctgtcttagt
    19621 agagaaagaa gcacaaaaag cagaggtgat cgcaaccgag gaaggtgctt ctgccgatgt
    19681 acgtgaggaa gtacctgtgg cagcgagtca agttatcagc gatgcaaata aagaaatcaa
    19741 tgtgttggta ctttgtgctg gcgcagggac aagtgcgatg ttagctaatg ctttaaaaaa
    19801 aggtgcatct gaatcgaaag caccgatcag tgcaatggcg ggtgcttatg gttctcatta
    19861 cgaaatgctg caggattttg atatggtggt cttagcaccg caagtcaatt cttactatga
    19921 tgatatcaaa gaagacaccg atcgcttaga aatcaagctg gttgcgacaa aaggagcaga
    19981 atatatcgga ttaacgagag atcctgaaaa agctgttcag tttattctga accaatttca
    20041 ataaaggagg agccataatg aaaatgggaa tttggttgcg taaagctgtt ttatttgcag
    20101 ctgtgttagg ggctttgggg gctgtttcaa aaactgttga agcatctgcc agcgaattta
    20161 ttcgcggagc agatatttcg atcctgtcag atatggaaaa atctggtgca aaatattatg
    20221 aagacgatat ttcaaaagat gccttagaga ttttaaaaaa taatggtgtc aactatgttc
    20281 gactgcgttt gtggcaggat ccttataata gtaatggaga aagttatgga gcagggacaa
    20341 atgatctgga aacgacgatt gaccttgcaa aacgagcaaa aaatctggga cttaaggtgc
    20401 tgctggattt tcactacagt gatttttggg ttgatcctgg gaaacagaat cttcctaaag
    20461 catggcaagg tctgaccttt gaagaaatga atacggcatt gtacgactat acaaagaacg
    20521 ttctgtcgga aatgaagcag ctggacgttt atcctgatat ggttcagatc ggcaatgaac
    20581 tgaatagcgg aatgctctgg ccttatggca agtcttgggg agaaggcggg ggagaatttg
    20641 atcgattggc tgcttttttg aagtctggta tccaagctgt tcgagacact caacctaaaa
    20701 caactccagt aatgctccac ttagctgatg gtggcgatac aggagcattt acttggtggt
    20761 ttgatgaaat cacgagtcga ggtgtgtcat ttgatttaat tggagtgtcg tattatccgt
    20821 attggcacgg ttcaatgaat gaacttcaaa ccaatatgaa tgcgatcagc caacggtatg
    20881 gtaaagaagt gatcgtagtt gaaacagctt atgggcatac aacagcgaat gccgatacta
    20941 tgccaaatgc ttttggtgaa gccgaagcag ctgcgggggg atatgaacca acaccgacag
    21001 gacaagcaga gtatttgctg gatcttgccg atcggattca ggcagtaccg aataatcgcg
    21061 gggcgggctt cttctattgg gaacctcttt ggtacaacgg aaatgtgagt tgggcaacac
    21121 aagcaggtat gaattatctc agtgttcaat ccactatggg aaacgaatgg gacaaccaag
    21181 cgatgtttga ttttgctgga aacgccttgc caagcttgcg tgcgtttaaa caagcagggg
    21241 cgcaaataaa tctagttaaa aatgcaagtt tcgaaagcga tggaccaacg accagtcctt
    21301 ccaattggca aacctggttt caacaaggaa ccgctaaggg tacgatcaaa acggaagcgg
    21361 gcactttttt tggggactac aaactaactt tttggcagga tcaagcatac gaggcatcag
    21421 cttatcaaaa tgttagtggc ataagcaaag gaacttacac attgagtgcc tatgtaatgt
    21481 ctgggggaga gtttgatact aatcagttat atatcaaaaa ttatggtgga gcggaactaa
    21541 atactgcggt tgttaaaaca agtgaatgga caaaaattac aatcaaagac attccaatta
    21601 caacaggtgt ttgtgaaatt ggggtttata gcaaggcttc aggaaatgct tggacgaacg
    21661 ttgattttgt ttcattggtg aaacaataga ctgcggatcg tttcgattca agatcggagg
    21721 ttcccttcag ccacatcgtt agcgtagaag gcaggaatcg aacctgcacc tcgcctttgc
    21781 caaaaataac cagaaacgat ttccagtcat ttttaccaaa acgagtaaag caccagactt
    21841 catctacact ttgtattatg ctaaagagag ttcaacgatt tcgtaagaag caatgaaaaa
    21901 attgcaaaaa gttgtgcaga taacataaag tttgtttaag aaagggtaac cttagaattc
    21961 cgtcgtgaat atgatttgag cacttagatg gatagaaaaa agttctcgga aaacagttcg
    22021 tttttcgaga acttcttttt atgataagaa tgaaacaaat taacttatat tatagtctct
    22081 tttggtaggg aaccatctta tttttaatga aaatcaagca ttttagctat tgaagaatgt
    22141 tgatttatag ggattccccc tccatattat gttggctctt tattcccact ccactgttgc
    22201 tggtggttta gaagtgatgt catacacgat acggttgacg tgggctactt cgtttacgat
    22261 acgtacagag attttttgca agacttccca tgggatacgg gcaaagtcag cggtcattcc
    22321 gtcgattgaa gttacagcac ggataccgac tgtgtagtcg taagtacgtc cgtcacccat
    22381 aacaccgaca gagcggatcc ctggaagaac agtgaaatat tgccagatat cacggtcaag
    22441 tccagcagct gcgatttctt cacgcaagat agcatcagat tcgcgtacga tttctagctt
    22501 gtcttcagtg atttcgccaa gaacacgaat accaagacca ggaccaggga atggctgacg
    22561 ccaaacgatt gaatcaggca tgcctaactg agtccctaat tcacggactt cgtctttgaa
    22621 taatgtgttt aatggttcga tcaattcaaa ttgcatatct tctggcagtc cacctacatt
    22681 atggtgagat ttgatcgtct gtgctgtttc tgtaccagat tcgataacgt cagtatacaa
    22741 cgttccttgt gctaggaaag agacgccttc ttcaccagca agttttgttg cttcgtcatc
    22801 aaaaacatat acgaattcat tcccaatgat tttacgtttt tgttctgggt cagatacacc
    22861 tttcaacttg tctaagaagc gatctcttgc attgactttg atgatgttca agccgaattt
    22921 tccgcctaga ctttccatta cttgttccgc ttcgcctttg cgtagcagac cgtggtcaac
    22981 aaagatagat gtcaattggt caccgatcgc tttttgtaaa agaaccccaa cgacactaga
    23041 atcaacgcca cctgaaagac caagcaagac ttttttgtca ccaacttgtt cacggatttt
    23101 ttctacttgc atatcgataa agttatccat tgtccagtta ccttcacagt gacaaacatc
    23161 aaaggcaaag tggcgcagca attcgattcc gtattcggaa tgacgtactt ctgcatggaa
    23221 ttggatacca taaaaattac gtgttgtgtc ttggatagaa gcaatcggac aatctttgct
    23281 tgttccgact gtttcaaagc cttcaggaac tttagttacc aagtcaccat ggctcatcca
    23341 tactgtttgt tgttttggtg tttctttaaa caagacagct tgatcgcttg ttacttctaa
    23401 caaggctttc ccatattcgc ggtttttagc aggctctacg gaaccgccaa gtttgtacgt
    23461 gatcaattgc ataccatagc aaatccctaa tacaggaata cctaaattga agatctcagg
    23521 atcgatccca aatgcattct catcatatac actgtttggt ccgccagaaa gaatgattcc
    23581 ctttggattc atcgctttta cttcttctgc agtaatgcgg tggctcaata attcggaaaa
    23641 gactccaaga tcacgaatac gtcttgtgat caattggtta tactgactac cgtagtctaa
    23701 tacgataatc ttttcaacgt tcgtcatatc ggcaacgttt gtcaaattta ttacccctat
    23761 ccctttaaat tttatctcaa aatagtcaaa ctattttaaa atgctaatat tttcgcatga
    23821 aaatcgaatc gatgatatga ttttcagttt tatggagaat tatatcggct cttcctcgtg
    23881 taggcaagat atattcctgt aaatttttca aattcacatt tttccataca tcttttgcca
    23941 ttttaaaggc ttcttcacga tctccgatag caaaacgata ataataattc tctggatttt
    24001 ggaaagccgt atccagtaac gcaccaaaac gatccaaata ccatttttcg atcagcacgg
    24061 gatcggcatc gacatagaca gaaaaatcaa aaaagtcgct gacgtagatt tgctgattcg
    24121 caggtaattg aagcgtattg atcccctcaa cgatcaagat atcaggctgc tggatcaatt
    24181 cgtacttgtc tggaatgata tcatacacat catgtgagta aacaggtact tttatctctt
    24241 cctgaccaga ttttacacgg ttcaagaaat cgatcagcat ctccatatca taactttcag
    24301 gaaatccttt acggtccatg attccacgct cttttaatat acgatttgga taaagaaatc
    24361 cgtcagtcgt gatcagctga acgtttcttc gtttgaaggt acgagacaaa atcgtctgca
    24421 gcagtctggc tgttgtactt tttccaacgg ctacacttcc ggcgatcccg ataataaaag
    24481 gcggtacttc tacgtattta tgaagaaata aacctttact caagaacaaa gattcaaatt
    24541 ctttcatata aagataaatc agatgagcaa gaggtacata gatatctcga acatcctgca
    24601 tggaaatgcg gtcattcaaa cttttgatat tatctagttc atcttgtgtc aaaggtgctt
    24661 gcccattacg ataaaattct cgccattctt ctcttgccac acggtagtag ttcattggat
    24721 cattcatatt agcctcctgc gttctcccat caatggaact attttaacac aaaataaacg
    24781 caaaccccac cacttaagag agattgccga tctttttgta taatctttat aatagaaaga
    24841 aacggagtct tattcttgac ttgcgcacgg atacataaaa ggatagactg ttgtcatgaa
    24901 agaaatgaga aggatggaag acatgacaaa tcattactat tctgaacgcc cagatacggc
    24961 gcacgatttt gaacaatggg cttttgagtt aaaaggcaga acctttcaat ttgtaacaga
    25021 ttctggtgta ttttcacgtg aaacagtgga ttatggttca cgtgtcctga ttgatgcttt
    25081 cgattgggaa gagttacctg atgaaggaaa gatcttagat gtgggatgcg ggtatggacc
    25141 gattgggtta gctttggcat ttgcgacaca gcggtttgtt gaaatggtcg acattaatga
    25201 acgagcggta agtctagcgc aaggaaacgc aaagcgtaac caaatcgaac atgttgatat
    25261 ccatcaatcg aatatctatg aagcagttca tgaagaaacc tatgcagcta tcgtaagcaa
    25321 tccgccgatc cgtgctggaa aaaaagttgt acatgaaatt ttgacaggcg catatcctcg
    25381 gttgaagaag ggcggtacac tgacaattgt gatccagaag aaacaaggag cgccgagtgc
    25441 gcaaaagaaa atggaagaaa catttggtaa tgcagaaatc gtcacaaaag ataaaggata
    25501 ttatatcatc aaaagtgtga aagaataact agctgaaaaa tagtggagcg acgaaagagt
    25561 ggaactgggc gtgcatgtac gggtacgtat cagttctttt tttgttgaga agtttttaaa
    25621 aacaaagaac tattttttct tcaaaaagaa atagtaggaa cagagtcgtt gttttttacg
    25681 ataaaataaa taagatacag aacttgtata gaaggctgcc aagtcggtag ataaaggagg
    25741 tttttatcaa aaaacatact ttcctttttt ctctattctt ttcttggggg ttttggtaca
    25801 ctgataggga atttagatag aagggagcca ataggatgaa gctgaccatc agagatatcg
    25861 cagaaatggc aggcgtatca gtaacaactg tctcacagat tttgaataac aaaggaagca
    25921 gatttagtga taagacaaga aaaaaagtgt tgtcgatcgt gaatgaacat cattataagc
    25981 ccgattattt cgcttcaaat gtgatcaatc gacattcaaa gaccatcgga atgatcgtcc
    26041 ctgatgtcac tgatttcttc ttctccaaac tgatcgaagg tgtagagagc tatttgaatc
    26101 ctttgggcta tgtgatcatg ttatgcaatt ctcgtcatag ccaagaaaat gagatcaagt
    26161 atttacaaga gctttcccac cgctcagtgg acggaatctt gctggccaca ccaaatattt
    26221 tacctgagaa atatgcatta gacagcggtt tttatcaaaa aatgccaatc attttaatcg
    26281 atcgcggatt gaatcgtcgg gataacggac ggcttatcgt aaaggaatat gagggcgcct
    26341 atcaagctgt ttcgctgttt attgaaaatg gccatacgaa gatcggcatg ctgaaggaaa
    26401 ccactggata ctatcaatta gaagaacgtt tcaacggata tcgtcatgcg ctgaaagatc
    26461 atggcctgcc ttttaatggg aaatttgttg aacatggaga gttgactgtc caaggtggat
    26521 atactgcatc aaaaaaatta ctcaaacata aagatatcac ggctattttt tgcgggaatg
    26581 atgccatggc gatcggatgc tatcaggcga tcgatgaatt gggcaaaaaa atcccagaag
    26641 atatctcagt cataggattc gatggattaa agctgtcaga atacatgatt ccgcaattga
    26701 cgacagtgca gcagccaagt tttgatattg gattttatgc tgcacgtttc ctgatcgata
    26761 cgatcgaatt tcctcaaaga aaagttccga acaaagtgtt cgaaacaaaa ttaattattc
    26821 gtgagagcgt aaaagtattg acaaagggct aactcggcgg tatattgctt atgtaagcat
    26881 ttacaaaggc tagttagtgc tttttttatt tttagtaaac cagtttacta aagtggttta
    26941 cctttcctaa aaataatgta taataccgaa tgaagacaag gaggatcagg caatgcgaat
    27001 ggtggatctg attgaaaaaa agagggatgg aaacgagctg tcgaaagagg aaatcgagta
    27061 catcgtcacg aactatacga atggaaagat tccagattat caagtcagtg cattattgat
    27121 ggcaattttt tatcaagaca tgacaaatga agaaatcacg aacttaacat tggcgattgc
    27181 taattctggt gatgtcatcg atttgagttc tcttgaagga ataaaggtag ataagcattc
    27241 gacaggtggc gttggtgaca cgaccacatt gattttagcc cccctagtag ctagtgtcgg
    27301 agtgacagtg gctaaaatgt ctggacgcgg tctaggttac acaggtggta cactagacaa
    27361 gctagaagca attcccggat ttcagatcga gctttcggat gaagaatttg tacgcattgt
    27421 taatgaaagt aaagtagcgg taattggtca atcaggaaac ttggcaccgg cggacaaaaa
    27481 attgtatgcc ttgcgtgacg tcacagcgac cgttgattct cttccgctga ttgccagttc
    27541 gattatgagt aaaaaaatcg cggcaggagc agatgcaatc gttttggatg tgaccactgg
    27601 agacggtgct ttcatgaaga acatcgaaga tgcaagacgt ctggcaaaga ccatgacgag
    27661 cattgggaaa ctggctaacc gagaaacagt ggcggttatc tcagatatgt cagaacctct
    27721 tggcgaagct atcggaaata gtctagaagt tgtcgaggca atcgaaacat tacaaggaaa
    27781 tggtccagaa gatctagtag aaatgtgcta cgccttagga agccaaatgg ttgttcttgc
    27841 tggcaaagct aagacaaccg atgaagcaag aacgttgctt caagaagctc tggaatcagg
    27901 taaagcgtta gcaaaattca aagaaatgat ccaaaatcaa gggggagacc cagcgatcgt
    27961 ggaacatcca gaacgaattt tgactgcacg ctacacaatg gagcttcctg ctaaacaaag
    28021 cggagttgtg tcaaaaatcg tggcaaatga attaggaatt gctgcaatga tgctaggtgc
    28081 tgggcgtaaa accaaagaag aagatattga tcacgctgtt ggattgaaac tgcataaaaa
    28141 aattggtgac actgtgacaa aaggtgagtc actattgacg atctatagta acgacgaaga
    28201 aatcacttct gtcattgaat tattatataa aaatattgaa attggtgaat cggcaatgaa
    28261 gccaacgttg attcatgaca tcatcacaga ataaaggaaa aaagcggact ttaccgtttt
    28321 aaagaaaagg ggaaaagaaa tggaactaaa ccgaatgatc gaccatacta ttttgaaagc
    28381 agacgcatct aaagaagctg tgatgcaaat tatcgaagaa gcaaaaaaat accattttta
    28441 ttctgtctgc atcaatccag catgggttgc gctagcgaaa gaacaattac aaggtgagcc
    28501 agtagccgta tgtacagtaa tcgggtttcc tctaggagca aacacacccg agacaaaagc
    28561 atttgaaaca acagatgcca tcaataacgg tgcagatgaa gtcgatatgg tcatcaatat
    28621 cggtgctttg aaatcaaagg atgatcaaca agtccaaaaa gatatcgaag cagttgttga
    28681 agcagcaaaa gatcgtgcgc ttgtcaaagt tattattgaa acatcgttat tagatagaga
    28741 agaaatcatc cgagcttgcg aaatagcaaa agctgcaggc gctgattttg tcaaaacatc
    28801 aactggtttc tcaacaggcg gagcaaaggt agaggatgtc cgtttgatgc gtgaaactgt
    28861 aggaccagaa atgggtgtca aagcttccgg cggtatccat aacgaagaag aagcaatggc
    28921 aatgatcgaa gctggtgcta caagaatcgg aacaagcgca ggagtagcaa tcgtatctgg
    28981 ttcaactggt gaaggctatt aattttttca aaggagaagc tcatggcaga actcaaacaa
    29041 gaatggatca acacagctat tgaagcgtta gataaagcct atgttcctta ttcccatttt
    29101 ccggtaggcg cttgtctcgt aacggaatca ggcaagattt atcaaggcat caacattgag
    29161 aatgcatcat ttggattaac gaattgtgcc gagcgcacag cttttttcaa agcagtatct
    29221 gaaggagaaa aaacatttac gcatctagtc gtagcggggc acacccccga tcctatctct
    29281 ccttgtggtg catgtcgaca agtaatggca gaattctgtt cccctgatat gccggttacg
    29341 ctagtaggag acaatggtgt gacgaaggca acgaccgttc gtgcactgct gccttatgca
    29401 tttactgaaa aagatctata actcatgttc attcagtcag ctgactgttg aaaaataaaa
    29461 caattatttt aggaggcttt acagaaatga aaaaagcaaa attatttggt ttaggtgctg
    29521 tcgcactagc agcagggtta tttttaggtg catgcggcaa caacggctct actgattcat
    29581 ccggtggcaa aagctcatca gacacaacaa ctgcagcatt gatcacagac acaggtggtg
    29641 tagacgaccg ttcattcaac cagtcagctt gggaaggact agaaaaatgg gggaaagatc
    29701 aaggactttc acgaggaaat gatggcttcc aatatttcca atcttcaaat gaatcagatt
    29761 acatcccaaa tattgaccaa gcattgaatg ctggattcaa aacgattttt ggtattggtt
    29821 ataaactaaa accagcgatc caagaacaag caacaaacaa tacaggtacg aactttgtta
    29881 ttatcgatga tgtcatcgat ggattagata acgttgtatc tgcaactttc aaagacaacg
    29941 aagcttctta tcttgcaggt gttgcagcag cttatacgac tgaaacaaac gtagtcggct
    30001 ttatcggcgg tgtaaaaggt gaagtaatcg atcgttttga tgctggattc aaagccggtg
    30061 ttgatgcagg tgcgaaagaa ttaggaaaag aaatcaaagt attgaatcaa tatgctggtg
    30121 acttctctgc tccagataaa ggacgttcaa ttgctcaagg tatgtatgca caaaacgcag
    30181 atatcatctt ccatgcttca ggcggtacag gtaacggtgt attccaagaa gcaaaatcat
    30241 tgaacgaatc aggcgacaaa aaagtttggg ttatcggtgt tgaccgcgac caatcagacg
    30301 aaggcgaata cacattaaac ggcgaaaaga aaaacttcac attgacatct acactgaaag
    30361 cagttggaac agtagtagaa gatttggcac aaaaatcagc ggacggtaaa ttccctggcg
    30421 gcgaacacac tgtttatgga ttgaaagaag acggtgttgg tctaacagaa ggacaacttt
    30481 ctgacgaagc gaaaaaagca gttgatgaag caaaagaaaa aatcatttca ggtgacgtaa
    30541 aagttccaga aactcctgaa gaaaactaaa accatttcta tagccttgtt agacacatgt
    30601 ttgacaaggc tatggtttga ttagaacaag aattgttcat ttatccaagc tgccttgtga
    30661 cagtttggat aagggaacaa ctccctgaag taatgtttgt ttcaaaagaa gaacgctcac
    30721 taagcgtgaa tcttagtcaa tcgttgccct agatagaaat cgttaggatg tgaaaaaaag
    30781 gtggttcaag aaaatattgt catagaaatg cagaatatta ccaaagaatt tggaacattc
    30841 aaggcaaatg acaacatcaa tttacaagta aaggcaggag aaattcatgc gttgcttgga
    30901 gaaaacggtg ctggcaaatc tacattgatg aacgtgcttt ccggattatt agagccgaca
    30961 tcagggaaaa ttttgatgcg tgggaaagaa gtaaagatca caagtccgac caaagccaac
    31021 caattaggga ttgggatggt ccatcagcac tttatgcttg ttgatgcctt tactgtaaca
    31081 gaaaacatcg tgttgggaag cgagcctagt cgcgcaggga tgcttgacca taaaaaagca
    31141 cgaaaagaga tccaaaaagt ttctgaacag tatggattgt cagtcaaccc agatgcttac
    31201 gttcgtgata tttcagttgg aatggaacaa cgggtagaaa ttttaaaaac actttaccga
    31261 ggagcagatg tactgatttt tgatgagccg acagctgtat tgacccctca agaaattgat
    31321 gaattaatcg tgatcatgaa ggaattagtc aaagaaggca agtcgatcat tttgattacg
    31381 cataagttag atgaaatcaa agcagtagct gaccgctgta cagttatccg ccgtggaaaa
    31441 ggaatcggta cagtcaacgt taaagacgta acctcacaac aattagccga tatgatggtt
    31501 ggaagaacgg tttcattcaa aacgatgaaa aaagaagcga agcctcaaga agtcgttttg
    31561 tctattgaaa atctagtggt gaaagaaaat cgtggattag aagccgtgaa aaacttgaac
    31621 ttagaggttc gtgctggcga agtacttggt atcgctggaa tcgatggaaa cgggcagtcg
    31681 gagttgatcc aagctttgac tggtttgcga aaggcagaaa gcggacatat caagctaaaa
    31741 ggggaagaca tcactaataa aaaaccccga aaaatcactg aacatggtgt aggacacgtg
    31801 ccagaagacc gtcataaata cgggttagtc ctagatatga cattatctga aaatatcgca
    31861 ctacaaacct atcatcaaaa accttacagt aaaaacggca tgctgaatta ttcagtaatg
    31921 aatgaacatg ccagagaatt gatcgaagaa tatgatgttc gaacaacgaa tgaacttgtt
    31981 cccgcaaaag ctttatcagg cggaaatcag caaaaagcaa ttatcgctcg tgaagtcgac
    32041 cgagatcctg atctgttgat tgttgcaaat ccaactcgtg gactggatgt aggtgcgatc
    32101 gaatttattc ataaacgtct gatcgaacaa agggacaaat acaaagcagt gttactgatc
    32161 agtttcgaat tagaagaaat tttaaatgtt tcggatcgta ttgctgttat ccatgaagga
    32221 gaaattgtcg gtatcgttga tccgaaagaa acatctgaaa atgaattagg tctattgatg
    32281 gcgggctata ccttagaaga agcacgaaaa gagttagaaa agggggctgg cgcacatgaa
    32341 tgatcgcagc gaaaaattcc gtaatttatt agtgcctgta ctttcagttg ttttaggatt
    32401 gatcttaggc gcgatattga tggctgcttt tggttttcag cccatccaag gttattcttc
    32461 tatgctagat gcagcccttg gttcgcaaag agggataggt gaaacgttaa ggcaagcaac
    32521 gccgttgatt tttacagcat taggtttttc tgtagctaac tcagctgggt ttttcaatat
    32581 tgggctttct ggacaagcac tttgcgggtg gatcgtcagt gtctgggtgg cacttgcgtt
    32641 tccagatctg ccaaaagttg ttttgcttcc gttatgcgtg atcttaggtg ccttagctgg
    32701 tgcgatggca gcggctgttc caggtttact tcgagcattt tttggaacaa gtgaagtgat
    32761 cgtaacaatc atgatgaact atatcttatt atacgtaagc acatttgctt tacatgaagt
    32821 gatgcctgaa agctatcgct caagtttgga ctcttccaat atgatcagtc aaaatgcctc
    32881 actgcggtta ccatggttaa cacaagcttt tggcggctct cgcgtaaact caggattatt
    32941 tttagcaatc atcgcgctta ttttcgtttg gctgctgatg aagaaaacga ctttaggatt
    33001 tgaaatccgt tctgttggat tgaatccttt tgcttctgag tatgcaggaa tgagtagtaa
    33061 acgtacgatc gttttgtcga tggtcatttc tggcgctttg gcagggcttg gcggtgtggt
    33121 tgaaggatta ggaacgtatc aaaacttctt tgttcaaacg acttcgctat ctattggatt
    33181 tgacggaatg gctgtttcct tgcttggttc tggatcagca atcggtattc ttttatcagc
    33241 gatcttgttc agtattttga agatcggtgg acttggcatg cagacaggag caggtgttcc
    33301 gtttgagatc gtcaatgtat cgattgcttt gattatcttc tttgtcggga tcaacttcct
    33361 gattcgtttc gtcatggcta aagtcttcca agggaaaaaa caagaagaga tcgttgcgac
    33421 aatagaatcg aaaccaacga atcaaaacca tgaagggggc gaactataat gtcgactatc
    33481 gaattaattg ccttgatcct tacttctaca ctggtttatt caacaccgct aattctaacg
    33541 tcactaggcg gaactttttc tgaacgaagc gggatcgtaa acgtagggct agagggaatc
    33601 atggtcatgg gtgcttttag ttccatcgtt ttcaacttga cgatgagtcc atcacttggt
    33661 tctatgacac catggatcgc tattttagtg ggtggcgtcg taggtatcct attttctctg
    33721 attcacgcag tagcaacagt cagtttgcgt gctgaccata ttatcagcgg gacagtaatt
    33781 aatctgatgg cacctgcttt aggtgttttc ttgatcaaag catggtatgg taaaggtcaa
    33841 actgataaca tcacgcagaa tctggggtat ttttctttcc caggattagc agatattccg
    33901 gtcattggac caattttctt taaaaataca ttcttacctg cttattgtgc tattttgatt
    33961 gctgtcctag cttgggtgat catcttcaag acgaaattcg gcttgcgcct ccgttcagtt
    34021 ggtgaaaatc ctcaagctgc cgacacatta ggtatcaatg tatatgctat gcgttattca
    34081 ggcgtactga tttctggctt gttaggcggt atgggcggtg ctgtttttgc tcagtcgatt
    34141 tccggtaact tttcagctgg tacgatcgtt ggacaagggt tcatctccat ggctgctatg
    34201 atctttggta agtggaatcc tttaggggca atgggtgcct cattgttctt tggttttgct
    34261 caaagcttga gtatcattgg cgcacaatta ccagttattt cttcgattcc gccagtattg
    34321 ctgcaaattg ctccatattt gctgacaatc attgtgctgg ttgttttctt agggaaagct
    34381 tctggtccaa aagcaaacgg gaagaactat atcaaatcaa aataatcttt tagcgaataa
    34441 aaagggtggg ttacgggaag tgtttttcgt ctcgcccttt ttgttttaaa ggcaataaaa
    34501 ataaaaagaa agaaggaata atcatgttta aacgcgtaca tctgatcgtc atggattcag
    34561 taggaattgg agaagcacca gatgccgata agttcggtga tgttggttct gatacattag
    34621 gacacattgc gaaagaagca ggattgaacg ttccacattt agagcagctt ggtttaggaa
    34681 cgatccgtcc tcttgaaggt gtggaatcag tgaaagatca tcaaggctat gcgacgaaat
    34741 tagaagaagt ttctgtaggg aaagatacga tgacaggcca ctgggaaatc atgggattga
    34801 atatcaaaac tccatttcgt gtgtttccta atggattccc tgatgatctg atcaaacaaa
    34861 tcgaagaatt ttctggccgt aaagtcgtat gtaacaaacc ttatagcgga acagctgtta
    34921 ttgacgatta cggtgaacac caaatgaaaa caggcgattt aattgtatat acttctgccg
    34981 atcctgtcct tcagatcgcg gcacatgaag aaatcattcc tttagaagaa ttatatcgga
    35041 tttgccaatt cgttcgtgat ataacaaaag atgatccgta tatgattggc cgaatcattg
    35101 cacgtccgta cgtgggtgaa ccaggagact tcacacgaac aagcaatcgc catgattatg
    35161 cgttgaatcc attcggacac acagtccttg attctttgaa agaagcagga aaggacgtga
    35221 ttgctgttgg aaagatcaac gatattttca acggacaagg aatcactgaa tctgtccgca
    35281 cgaaaagcaa tatggatggc gttgaccagc tgctgaaagt tatgaaaaac gatttttcag
    35341 gaatcagctt taccaattta gtagattttg atgcattata cggtcatcgt cgggatgtag
    35401 tcggctatgc gcatgcgatt gaagaatttg acttacgaat tccagaattg cttgaagcga
    35461 tggaagaaga cgacttgcta atgattacag ccgatcacgg aaacgatccg acgtttactg
    35521 gtaccgatca tacgcgagaa tatgtaccgt tattggttta cagtaaaaaa atgaaagaac
    35581 aaggggcatt gcctcaagga tattattcag atatttcagc aacgattgct gagaacttta
    35641 gtgtttcttc gactgaaaat ggcgaaagct tcttgaaatt attaaaataa tctttttaaa
    35701 taggagaaag caaatgacca agttaagcga gatgttacaa gaaacgaccc agtttatcct
    35761 tgataaagga gtaaaagaaa ttgattttgg tttgattcta ggttcaggat taggtgaatt
    35821 agcagatgaa atcgaagatg ctgtaatgat ttcttatgat gagatcccgt tctttcctgt
    35881 ttcgacagta gttgggcatg caggaaagct agtttatggg tctctggcag gcaagaaagt
    35941 gttagccatg caagggagat tccactacta tgaaggtcat tccatgcaaa cagtgaccta
    36001 tccagtacgt gtgatggccg gactaggggc ccattcagtc attgtgacta atgcatgtgg
    36061 tggtgtcaac gaatcatttg taccaggaga tttgatgctg attaccgatc atatcaattt
    36121 tactggtgaa aatcctttga tcggtccgaa tgaagatgca atgggcccta gatttccaga
    36181 tatgagtcaa gcttatacgc ctgactatca agcaacagca aaagcagcag cagaaaaact
    36241 aggtttggat cttaaagaag gcgtatatat gggctattct ggaccaactt atgaaacgcc
    36301 tgcggagatc cgaatgtctc ggacaatagg agccgatgca gtagggatgt cgactgttcc
    36361 agaagtcatc gtagcagctc acagcggatt aaaagtgtta ggaatctctt gtattaccaa
    36421 cttagcagca ggtatgcaag cgaatctgag ccatgaagaa gttgtagaaa caactcagag
    36481 agtcaaacaa tcttttaaag cattggttaa agaaacattg acattattgt aataggagga
    36541 acaataaaat gagtgtgcat attgaagcga aagaaggcga gatcgccgat aagattttac
    36601 ttccgggaga tcctttgcga gctaaatata ttgcggagac ttttttggaa gatccagttt
    36661 gttacaatca ggtacgcggt atgttaggct ttaccggcaa atataaagga gaacgtattt
    36721 ctgttcaagg aacaggaatg ggaatgccat cagccaccat ctatgcccat gaattgatcc
    36781 agtcgtatgg tgtaaagaaa ttgattcgag taggtacatg cggtgcattg tcaaaagacg
    36841 tccatgttcg tgatttagta ctagcacaag gtgcagcgac cagttcttcg atgatcgaaa
    36901 aaaatttcca agcctttcat tttcctccaa tcagtgattt caatctatta ctgaaagcat
    36961 atgagatagc taaagaaaag ggctatacgg ttcatgtagg aaatgttctt tcggaagatt
    37021 ctttctacaa agatgatttg acagagacgt tccaattagc tgaacttggc gttttaggag
    37081 tggaaatgga agcagcggct ctttattact taggcgcgaa ataccatgtg cagacattaa
    37141 gtttgatgac cgttagcgac catttgatca ccggtgaaga aacaacagct gcagaacgtc
    37201 aatctacgtt caatgaaatg atcgaagtag gattggaaac agcgatagca gaataagaag
    37261 gaaatgaaaa agctgccctg cattaagtaa taagaacatc caagcaaaaa tagctcctca
    37321 tgtttttgac ttggctggac ttattattgc aaatgtaagc ttttgaacac cgtttaccaa
    37381 gaggttgtga atctgccgtt taactctgag tcacaaggaa caatttcaaa aaacagcttt
    37441 tcatgtttga tgaatattgt gacttgtgac cgagaagttg gcaggagaaa accgtttata
    37501 cgaaaaaagg gactgtgaca aatcgtgtca cagtcccttt tttatagtgt tttttatagg
    37561 tagtattttt tagcaactgt taagtcatca tttaattcat aaacaagtgg ttgacctgtt
    37621 ggaatttcaa gatccatgat gtcttcgtca gagatacctt cgatatgttt agctagggcg
    37681 cgtaatgagt taccatgtgc agctactaaa acagttttgt tgtctagtaa tgctggagca
    37741 atttcatctt gccagaatgg caaagcgcgt tctaatgtca ctttcaagtt ttctccacca
    37801 ggaatatcgc gtttgtctaa cattgcgtaa cgacggtcat ttgctgcaga accttcgtca
    37861 gtttcttcca ttaatggagg aagagtatcg taagaacgac gccagatgtg cacttgttcg
    37921 tcgccgtatt tttcagctgt ttcttttttg ttcaaacctt gtaatttacc atagtgacgt
    37981 tcgtttaagc gccatgattt gatttgtggt acccaaagtt gatctgaata ttctaagatc
    38041 aagttacatg ttttgattgc acgagttaaa acagaagtgt aagcaacatc aaattcgatt
    38101 ccagcttctt tgattttgcg tccgccttct tttgcttctt cgattccttc tggtgctaga
    38161 tttacatcag cccaaccagt aaattggttc aatgcattcc attcactaag accgtgacga
    38221 gaaaaaacta attttggcat gtttagctct ccttttaaga taggtttata tttctgaaat
    38281 tttcagttct gttttattgt acaatttttt ggaagagatt tccatcgaaa aattagataa
    38341 gattctatga gaaaacaagt agagaatata aaaaaacgaa cagtgcccac gcaactgttc
    38401 gtttttaggt attagatgca taattgctcg atttcttttt caggtaaatt gatatattta
    38461 taacatgttc caactgaaac accgcatttt gaagaaataa attggatcgt ttcttttttt
    38521 tcataataga gacgtcggat ttttttgatc gtttttgcat cgatttttgg tcttccgccg
    38581 atttttcctt ttttacgagc ttcatctaat ccaactaatg tacgttcttt gatcaatgcg
    38641 cactccatgt ttgctaggtt ttccattaat tggaaataga tttttcccat ttcgcgcgta
    38701 tcgattcctt catcaagact aatcaagtgc agcccgcgat ctttgaataa ttgcgtgagt
    38761 tctgttaatt gccgcgtaga tttgcctaat cgatgaagtt ggcaaatgac tagtgcatcg
    38821 ccttgttgca tgtcagcgat cactgactcc aaatgaacag cagtagaatc attttgttcc
    38881 acttcatatg tctcatggaa aatttcttga caaccaaagt cagataattg atcaacctga
    38941 gttgttaaat caacgtcatt tatggttgtg cgtgcataac caataatttt catggtgatt
    39001 cctttctagc agtgattttc taactggata taacagtaaa aatgtagcat aaagtacgtc
    39061 aaaaagatag ttttttcgca aattttccgc tgtctttttt tattggatga tcaagcagtt
    39121 tttctttatt tacgacggat tgcgtcaaga atctcaacgc ctttttctga gccgacaact
    39181 actttagaaa ccatgtttag gaaaagccca tgttctacta ccccaacaag ttcatctaaa
    39241 taggtagcta agctttctgg ctgttcgatg acttctagat gcagatcaat gatgtaatga
    39301 ccaccatctg tggttaagac ttctccgttt tcgtctgtac gcaagacggg ttggaatctt
    39361 ttttcttcaa aaatatgaag tagctgttgg gagccataag ggatcacttc aactgggagt
    39421 gggaacttcc caagtttatc gactaattta gaagaatcaa cgatccaaat cgtttctttt
    39481 gagtacgtag cgactatttt ctcgaaaagc aaagcagcgc cgccgccttt gatcccttga
    39541 aaatcagcac tgatttcatc agcaccgtct attgtcaaat cgactacggg gacttcatca
    39601 atactcttta acggtattcc taatttttcc gcttgttctt ttgtcgcatt tgaagtagta
    39661 actcctgtga tacgtaaacc ttcttcacgc atacggcgac caatctcttc taccatataa
    39721 taggcggttg aaccggtccc gagacctacg atcatcccgt cctttacgaa tttcgcggct
    39781 tcaatgccag ctaattgttt taaattcatc tttgcgtcca ccttttttta gtttgagcat
    39841 cgtgctttcc tcactatttt agcgtgattt acggtaaaaa gataccattc ttttttattc
    39901 caataaagct ttttcaaaaa aaattgcatc tcttgttgac atgttttttc attcgtgata
    39961 ttctaacaaa ggtgctttat ctacaggttt ctgtgaagaa acgtgctgac tgtttatcaa
    40021 agcatatttg ataaggatgc agacattgca tttatttttt atcacaaaaa aagaaccacc
    40081 tggatgtgtg gaactagacg caaatcgtgg gaaggaggaa acaaggaatg cctacaatta
    40141 accaattagt acgtaaacct cgtaaatcaa aggtggaaaa atctaattca cctgctttga
    40201 acaaaggata taacagcttt aagaaaactc aaactaacgt aaactcacct caaaaacgtg
    40261 gggtagcaac tcgtgtgggt acaatgacac ctaaaaaacc gaactcagct ttacgtaaat
    40321 atgcacgtgt tcgtttgtca aacttaattg aagttactgc ttatatccca gggattggtc
    40381 ataacttaca agaacacagt gttgtattat tacgcggtgg tcgtgtaaaa gacttaccag
    40441 gggtacgtta ccatatcgtt cgcggtgctt tagatactgc tggtgttaac gaccgtaaac
    40501 aaagccgttc taaatacgga actaaaaaac ctaaagctta attttaatta ttaaacaaat
    40561 gaaacttgct tctactatat agaagtaatt actatgaaca atatttcgga aggaggagtt
    40621 acggatgcca cgtaaaggtc ctgttgcaaa acgtgatgtt ttaccagatc ctatttataa
    40681 ctcaaaatta gtaactcgct tgatcaaccg tgtaatggtt gacggaaaac gcgggattgc
    40741 agctaacatt atctataatg cctttgactt gatcaaagaa tctacaggta acgatccatt
    40801 agaagtgttt gaacaagcaa tgaaaaacgt tcaacctgtt cttgaagtta aagcacgtcg
    40861 tgttgggggt tcaaactacc aagtaccagt tgaagttcgc cctgaacgcc gcgttactct
    40921 aggtcttcgt tgggtagtaa actacgctcg cttgcgcggt gaacacacaa tggaagaacg
    40981 cttagcaaaa gaaatcatgg atgctgcaaa caacacaggt gcttctgtta aaaaacgtga
    41041 agacactcat aaaatggctg atgccaaccg tgcgtttgct cactatcgct ggtaagattc
    41101 tctctgtttg gtcggctttt ataagtcgac ctcagatagt gtccttattt ttagcaacta
    41161 gatagtaaag agaggagaaa caaggagaat gaccagagaa ttttctttac aaaacactcg
    41221 taatatcggt atcatggccc acatcgatgc tggtaaaacg actgctactg aacgtatctt
    41281 gtactatact ggtaaaatcc ataaaattgg tgaaactcat gaaggagctt cacaaatgga
    41341 ctggatggaa caagaacaag aacgtggtat tacgatcact tctgctgcta ctacagcaca
    41401 atggaagggt caccgaatca acatcatcga cacaccagga cacgtagact tcacagttga
    41461 agttgaacgt tcattgcgtg tacttgatgg tgctgtaact gttcttgatg cacaatcagg
    41521 tgttgaacct caaactgaaa cagtttggcg tcaagcaaca acttatggtg tacctcgtat
    41581 cgtatttgca aacaaaatgg acaagatcgg tgctgacttc ttatattcag tatcaacttt
    41641 acatgaccgt ttgcaagcaa acgctcaccc aatccaatta ccaattggtg cggaagacga
    41701 cttcacagga attatcgacc ttgtaacaat gaaggctgaa atgtatacaa acgatcttgg
    41761 aacagagatc gaagaaacag aaattcctga agaataccgc gaattagctg aagaatggcg
    41821 cgaaaaattg gttgaagctg tagctgaaac tgatgaagaa cttactttga aatacttaga
    41881 aggtgaagaa atcactgaag ctgagttaaa agaaggtatc cgccgcgcaa cagttaacgt
    41941 agaattctac ccagtactat gtggttcagc atttaagaac aaaggggttc aattactatt
    42001 ggacgctgtt cttgattact tgccatcacc gttagatatc ccagctatca aagggattga
    42061 ccctaaaact gatgaagaag tagaacgtcc tgcagacgac agcgctcctt tctcagcttt
    42121 agcatttaaa gttatgactg acccattcgt aggtcgttta actttcttcc gtgtgtactc
    42181 tggtgtttta caatcaggtt catacgtaca aaacgcaaca aaaggcaaac gtgaacgtgt
    42241 tggtcgtatc ctacagatgc atgccaactc tcgttcagaa atctctgaag tttacgctgg
    42301 agatatcgct gctgctgttg gtttgaaaga cacaactact ggggacacac tatgtgatga
    42361 aaagagtcta gttattcttg aatccatgga attcccagaa ccagttatcc aagtcgctat
    42421 tgaacctaaa tcaaaagctg accaagataa aatgggtgtg gctttacaaa aactatctga
    42481 agaagatcct actttccgtg ctgaaacaaa cgtggaaaca ggcgaaacaa tcatcgctgg
    42541 tatgggtgaa cttcacttag atatcatcgt tgaccgtatg cgtcgtgaat tcaaagttga
    42601 agctaacgta ggtgcgcctc aagtatctta ccgtgaaact ttccgtgctg gcactcaagc
    42661 cgaaggtaag tttgtacgtc agtctggtgg taaaggtcaa tacggtcacg tatggattga
    42721 atttacacca aatgaagaag gtgctggttt cgaatttgaa aatgcgatcg tcggtggggt
    42781 tgttcctcgt gaatacattc cagctgttga aactggtttg aaagatgcta tggaaaatgg
    42841 tgtcttagct ggctatccat tagtagatat caaagctaaa ctttacgatg gttcatacca
    42901 tgatgtcgat tcaaatgaaa ctgccttccg tgttgctgct tctatggcat tacgtgcagc
    42961 agctaagaaa gctaatccag taatccttga accaatcatg gctgtagaag ttgttatccc
    43021 tgaagattat ttaggtgatg ttatgggtca cgtaactgct cgtcgtggac gtgttgaagg
    43081 tatggaagct cgcgctggcg gccaacaagt tgttcgtgcg atggttccac ttgctgaaat
    43141 gtttggttat gctacaactc ttcgttctgc tactcaaggt cgtggtacat ttacaatgac
    43201 atttgaccac tacgaagatg taccaaaatc agtacaagaa gaaatcatta agaaaaacgg
    43261 cggaaaagct gaataatttc ttaaaaccgg atagtcctgc aaagagatta tccaaagaga
    43321 ctatattttt aatatagttt cgtgtaaaat aattatcgat gatatgggca tgtggtgtac
    43381 cttgtgtaca tcatatacct atcagacaaa taaaaaatat attgtatttt taggaggaca
    43441 ttttaaaatg gcaaaagaaa aatttgaccg ttctaaacca cacgttaaca ttggtactat
    43501 cggacacgtt gaccatggta aaactacatt aacagctgca atcacaactg tactatctaa
    43561 gaaaaacggc ggccaagcta tggcttacga tcaaatcgat ggtgctccag aagaacgcga
    43621 acgcggaatc acaatctcaa cagctcacgt tgagtatgaa acagatacac gccactacgc
    43681 tcacgttgac tgcccaggac acgcggatta cgttaaaaac atgatcactg gtgctgctca
    43741 aatggacgga gctatcttgg tagtttctgc tgctgacggc ccaatgcctc aaactcgtga
    43801 acacatccta ttgtctcgtc aagttggtgt tccttacatc gttgtattct tgaacaaagt
    43861 agacatggtt gatgacgaag aattactaga attagttgaa atggaagttc gtgacctatt
    43921 aacagaatac gaattccctg gtgacgatgt tcctgtagtt gctggatcag ctttgaaagc
    43981 tctagaaggc gacgcttcat acgaagaaaa aattcttgaa ttaatggctg cagttgacga
    44041 atacatccca actccagaac gtgacaacga caaaccattc atgatgccag ttgaagacgt
    44101 gttctcaatt actggacgtg gtactgttgc tacaggtcgt gttgaacgtg gacaagttcg
    44161 cgttggtgac gaagttgaag ttgttggtat tgctgaagaa acttcaaaaa caacagttac
    44221 tggtgttgaa atgttccgta aattgttaga ctacgctgaa gctggagaca acattggtgc
    44281 tttactacgt ggtgttgcac gtgaagacat ccaacgtgga caagttttag ctaaaccagg
    44341 tacaatcaca cctcatacaa aattctctgc agaagtatac gtgttgacaa aagaagaagg
    44401 tggacgtcat actccattct tcactaacta ccgtccacaa ttctacttcc gtacaactga
    44461 cgtaacaggt gttgttgaat taccagaagg aactgaaatg gttatgcctg gcgacaacgt
    44521 aacaatggaa gttgaattaa tccacccaat cgctatcgaa aacggtacta aattctcaat
    44581 ccgtgaaggc ggacgtacag ttggtgccgg tgtcgttaca gaaatcaaag cttaattcac
    44641 ttaagtttta aagtaaagca acatttattc ggacgtaatc gctcggaacg aaggtcttag
    44701 gatctttgtt ccgagctttt ttatttattg caggatcagg aaaaaagaca ggagtattat
    44761 aaatagaaga aacacgaaat aaaagagtca gtattttcta aatgaaactt tatgaaaaca
    44821 aaatgttttt tacaagaaaa agccttgctt tgtgagttta tatatattat aatgacaaag
    44881 gtgcgtttac tttagtgaat tgcacagacg taatcagaat gatttcgtcg gctaagaaac
    44941 gagaggttgc gacacgcccg gacgctttgc cacgaacgag cgtgacggaa attttcgtgg
    45001 agctatgtct acttttcaaa ataggcgaag gagggaacaa gatggcaaaa caaaaaattc
    45061 gtatccgttt aaaagcgtat gaacaccgta ttttagatca atcagcggat aaaatcgtgg
    45121 aaactgcaaa aagaactgga gctagcgtgt ctggtccaat tccattacca actgaacgca
    45181 gtctttacac aatcatccgt gcgactcata aatacaaaga ttcacgcgaa caattcgaaa
    45241 tgcgtacaca caaacgtctt atcgacattg tgaacccaac accaaaaact gttgatgctt
    45301 tgatgaagct agacttacca tctggtgtaa acatcgaaat caaattataa aactaaaaca
    45361 aaaataatgg aggtgtactc atgaccaaag gaatcttagg gaaaaaagtg ggaatgacac
    45421 aaatcttcac tgaatctggt gaattaattc cagtaactgt tgtagaagct actccaaacg
    45481 tagtactaca agtgaaaaca atggaaacgg atggctacga agctattcaa gttggttacc
    45541 aagatatgcg tgaagtttta tcaaacaaac ctgcgaaagg tcatgttgca aaagcaaaca
    45601 cggctcctaa gcgcttcatt cgtgaattca aaaatgttga gctaggagaa tatgaagtag
    45661 gaaaagaaat tacagtagat gttttccaag caggagacat tattgatgta acaggtacta
    45721 cgaaaggtaa aggattccaa ggggttatca aacgccacgg acaaagccgc ggaccaatgg
    45781 ctcacggttc tcgttaccat cgtcgtcctg ggtcaatggg tccagttgca cctaaccgtg
    45841 tatttaaaaa caaacgtctt gctggccgta tgggtggcaa ccgcgtaact atccaaaact
    45901 tggaagttgt acgtgtcgat gcagaaagaa atgttatctt gatcaaaggt aatgttcctg
    45961 gagctaaaaa atctttaatt actatcaaat cagctgtgaa agctaaataa ttgtaggaag
    46021 aaaggaggaa ctaaggaatg ccgaatgtag cattattcaa acaagatgga agccaaaacg
    46081 gtgaaatcac acttaacgaa gaaatcttcg gaattgaacc taatgaaagt gttgtttacg
    46141 atgcaatcgt aatgcaacgt gcgtcattaa gacaaggaac acacgcggtt aaaaaccgta
    46201 gcgctgttcg cggcggcgga cgtaaaccat ggcgtcaaaa aggaacaggt cgtgcacgtc
    46261 aaggatctat ccgctcacca caatggcgtg gaggtggcgt agtctttgga ccaacaccac
    46321 gttcttacag ctacaaactt cctaaaaaag ttcgtcgttt agcaatgaaa tctgtattgt
    46381 cagaaaaagt tgctgaaaac aacttggtag caatcgaagg cttgaacttc gacgcaccaa
    46441 aaacaaaaga attcaaacaa gttcttgcta acttatctat cgactctaaa gtattggttg
    46501 ttctagaaaa aggtaacgac tttgctgctt tatcagcacg taacttacca aacgtttctg
    46561 tagtaacttc tgataatgtg agtgtgctag acgttgtatc aaatacaaaa gtgttagcaa
    46621 cacaaactgc tcttactcaa attgaggagg tgcttgcata atgaacttac tagacgtaat
    46681 caaacgccca gtgatcactg aaaaatcaat gcttgccatg gatgacaaga aatacacttt
    46741 tgaagtggac actcgcgcaa acaaaacttt agtaaaacaa gcggtagaag ctgcttttga
    46801 cgttaaagtc aaaaacgtga acatcgtgaa cgtacgtgcg aaattcaaac gtatgggcaa
    46861 atatgcagga tatacaaaaa aacgtcgcaa agcgatcgtt acattgactg aagattcaaa
    46921 agaaatccaa ttattcgaag ctgctgaata agcactaata gataactcat taaattagga
    46981 gggaaaaacg tggcgattaa aaagtacaaa cctacctcaa atggtcgtcg taacatgaca
    47041 ggttctgatt ttgctgaaat cacaacatca acacctgaaa aatcattgtt acaaccattg
    47101 aaaaaccatg ctggacgtaa caacaacggc cgcatcactg tacgtcatca aggtggcgga
    47161 cacaaacgcc aatatcgcgt gatcgacttc aaacgtaaca aagataacgt tgttgcaact
    47221 gttcaaacga tcgaatatga tccaaaccgt tcagctaaca tcgcgttagt acactatgaa
    47281 gatggagtga aagcttacat cttagcacct aaaggactag aagtgggtat gaaacttgtt
    47341 tcaggtccag aagcagatat caaagtcgga aatgctcttc cattagaaaa cattccagtt
    47401 ggtactgtta tccacaacat tgaaatgaaa cctggtaaag gtggacaatt gatccgttct
    47461 gctggtacaa gtgctcaagt acttggtaaa gaaggcaaat acgtattgat ccgcttgaac
    47521 tctggtgaag ttcgtatgat cttagctgca tgccgtgcta caatcggttc tgtaggtaac
    47581 gaacaacacg aattaatcaa catcggtaaa gctggacgct ctcgttggat gcgtaaacgc
    47641 ccaactgtac gtggtagcgt aatgaaccct aatgatcacc cacacggtgg tggtgaaggt
    47701 aaagctccta tcggacgtaa agcaccagtt tctccttggg gtcaaccagc tcttggttac
    47761 aaaactcgta acaaaaaagc taaatcagac aaacttattg ttcgtcgtcg caaaaataaa
    47821 taattacttt gaccatgtgt cgcacatttt gagaggaggt tcaccatggg tcgtagttta
    47881 aagaaaggac cttttgtcga tgagcattta atgaaaaaag ttgaagctca agcaggggtt
    47941 gaaaagaaaa aagttatcaa aacttggtca cgccgttcaa cagttttccc aagttttgtt
    48001 ggatatacca tcgcagttta tgatggacgt aaacatgtac cagtttacat ccaagaagac
    48061 atggtaggac acaaattagg tgaatttgca cctacaagaa cttatcgtgg acatgctgct
    48121 gacgataaga aaacgaaacg ctaatttgag gggaggataa atccaaatgg cagaacaaat
    48181 cacatcagct aaagcaactg caaaaacagt tcgcacttca cctcgaaaag cccgtttagt
    48241 aattgacctt atcagaggta aaagtgtcgc agatgcaatt tcaatcttga aattcacacc
    48301 aaacaaatct gctggaatca ttgaaaaagt attgatgtca gcagttgcga atgcagaaaa
    48361 taactttgac ttagatgttg aaaacttggt agtaagcgaa gcttttgtta acgaaggacc
    48421 tacaatgaaa cgtttccgtc ctcgtgcaaa aggatcagct tctccaatca acaaacgtac
    48481 aagtcatatc acggtagtcg tatcagaaaa ataaggaggg ataatcagtg ggtcaaaaag
    48541 tacatccaat tggaatgcgt gtaggcatca tccgcgactg ggatgctaaa tggtatgctg
    48601 aaaaagagta tgctgagttc ttacacgaag atttaagaat ccgtaaattt atttcaacta
    48661 aacttgctga tgctgctgta tcaacagttg aaatcgaacg cgcaacaggt cgcgtaaaca
    48721 tttcaattca cacagctaaa ccaggtatgg ttattggtaa aggcggttct gaagtcgaaa
    48781 gcctaagaaa agaattaaac aaattaactg gtaaaagaat ccatatcaat atcgtggaaa
    48841 tcaaaaaacc agatctagat gcaaaattag taggtgaagg aatcgcacgt caattagaaa
    48901 accgtgttgc tttccgtcgc gctcaaaaac aagccatcca acgctcaatg cgtgctggcg
    48961 ctcaagggat caaaactcaa gtatctggtc gtctaaacgg agcagatatc gctcgttcag
    49021 aaggatactc agaaggaact gttcctcttc atactttacg tgcggacatt gattacgcat
    49081 gggaagaagc agatacaaca tacggaaaac taggagttaa agtgtggatc tatcgtggag
    49141 aaattcttcc aactaaaaaa aacactgaga aaggagggaa ataatcatgt tagtacctaa
    49201 acgtgtaaaa caccgtcgtg aattccgcgg aaaaatgcgc ggggaagcta aaggcggaaa
    49261 agaagtagca ttcggtgaat acggtttgca agctgttgat tcacattgga tcacaaaccg
    49321 ccaaatcgaa gctgctcgta tcgcaatgac tcgttacatg aaacgtggtg ggaaagtatg
    49381 gattaaaatt ttccctcaca aatcttatac tgccaaagca attggggtac gtatgggttc
    49441 tggtaaaggg gcacctgaag gatgggttgc accagtaaaa cgtggtaaaa tcatgtttga
    49501 aatcgcaggc gttcctgaag aagtagctcg tgaagcgtta cgtctagctt ctcacaaatt
    49561 accaatgaaa actaagatcg taaaacgtga ggaaatgggt ggtgaatcga atgaaggtta
    49621 aagaaatcag agaattaacc actgccgaaa tgcttgatca agaaaaacaa ttaaaagaag
    49681 aattgtttaa tcttagattc caattagcaa caggtcaatt agaaaacact gcacgtatta
    49741 aagaagtacg taaatcgatt gcacgcatca aaactgtgtt gcgtgaacaa gctaagtaat
    49801 tatggaagga ggccattaag cgtatgactg aaggaagaaa tcaacgcaaa gtttatcaag
    49861 gtcgcgtggt ttcagacaaa atggataaaa caattaccgt tgctgtcgaa acaaagaaaa
    49921 accaccctat ctatggcaaa cgcatgaact attctaagaa atacaaagtg catgatgaaa
    49981 acaacacagc aaaagttggc gacatcgtga gaattatgga aactcgtcca ttatcagcta
    50041 caaaacgttt ccgtttgttg gaagtagtcg aagaagcagt tattatctaa taacacgaga
    50101 ttttcctata tagattgaat gcaaaatctg aaaggaggat acacaacgtg atccaagcag
    50161 aaagtcgttt aaaagtcgct gataactcag gcgcacgtga aattttgacg atcaaagtcc
    50221 ttggtggatc tggtcgtaaa actgctaaca tcggtgatgt gatcgttgct acggttaaac
    50281 aagcaacgcc aggtggggtt gtcaaaaaag gtgacgtcgt taaagccgtt atcgttcgaa
    50341 caaaatcagg agctcgtcgt acagacggtt catatattaa atttgatgaa aatgctgcag
    50401 tgattattcg tgatgataaa agccctcgtg gaacacgtat ctttgggcca gttgcacgtg
    50461 aactacgtga aaacaacttc atgaagatcg tttcactagc accagaagtt ttataatctt
    50521 gagacactta tcaaaggagg tgcgaaacag ttatgtttgt taaaaaaggc gataaagtta
    50581 aagttatcac tggaaaagac aaaaacaaag aaggcgttgt actagcagcg ttcccgaaaa
    50641 aagataaagt tattgtcgaa ggtgtaaatg tcgttaaaaa acaccaaaaa ccttctcaag
    50701 ctgctcctca aggtggaatt gttgagatgg aagcgccaat tcatgtttct aacgtgatgg
    50761 tggttgactc tactggtgta gctggccgag ttggctacaa agaagtagac ggcaaaaaag
    50821 tccgtgtttc taaaaaaacc ggtgaagttt tagataaata aaagtttaag gaaggagggt
    50881 cttactgaat gaaccgccta aaagaaaaat atcttaaaga agtaactcca tcattgatgg
    50941 aaaaatttaa ctatagctct gttatgcaaa cacctaaagt tgaaaaaatc gtcatcaaca
    51001 tgggtgttgg tgatgcggta tcaaacgcta aaaacttaga taaagcagtt gaagaattaa
    51061 ctttgatcac tggtcaaaaa ccaatgatta caaaagctaa aaaatcaatc gctggattcc
    51121 gtttacgtga aggaatgcca attggtgcaa aagttacatt gcgcggagaa agaatgtacg
    51181 aattcttaga caaattggtt tctgtttcac taccacgtgt acgtgacttc catggtgtaa
    51241 gtaaaaaagc ctttgacggt cgtggaaact atactttagg tattaaagaa caattgatct
    51301 tcccagaagt agattatgat ttagtagata aagtacgtgg tatggacatc gttattgtaa
    51361 caacagcaaa ctctgatgaa gaatctcgtg agttgttgac acaattaggc atgccattcc
    51421 aaaaataatt aaaggaggcg aactacgtgg ctaaaaaatc aatgattgct aaaaacaaac
    51481 gtcctgcaaa attttcaaca caagcttata ctcgttgcga acgttgcgga cgtccacatt
    51541 cagtttatcg taagttccat ctttgccgta tttgcttccg cgaacttgcc tataaaggtc
    51601 aaattcccgg cgtgaagaaa gctagctggt aaaaagtaaa ttcgcataag aaggaggtaa
    51661 aagatcaatg gtcatgacag atccaattgc agattttcta actcgtatcc gtaacgcaaa
    51721 catggttaaa catgagtctt tagaagtacc tgcatcaaaa attaaacgtg acatcgctga
    51781 aatcttgaaa cgtgaaggtt tcgttcgtga tgttgaatac atcgaagatg acaaacaagg
    51841 cgtgatccgt gttttcctaa aatttggtaa aaacggcgaa cgtgttatca caaacttaaa
    51901 acgtatctct aaaccaggtt tgcgtgctta tgtcaaagct gacgaagtac caaaagtatt
    51961 gaacggttta ggtatcgcaa tcatttcgac ttctgaaggt gttgtcactg ataaagaagc
    52021 tcgtgaaaaa aatatcggcg gcgaagttat cgcttacgta tggtaatcaa gtagaatcta
    52081 caaggaggtg tctttaagtg agccgtatcg gaaataaagt cgttgttatc cctgaaggag
    52141 taacagttac tcaagacgga aacaacgtta cagttaaagg accaaaaggt gaattgactc
    52201 gtactttttc agcagatatc aaaatgaata tcgaaggaaa cgaagtaact ttcactcgtc
    52261 caaacgacag taaagaaatg aaaacaattc atggaacaac tcgtgcgaac ttcaacaaca
    52321 tggttgtagg tgtcagcgaa ggtttccaaa aagcgttaga actaatcggg gttgggtacc
    52381 gtgcacaatt acaaggatca aaacttgtat tgaacgttgg ttactcacat ccagtagaaa
    52441 tcacaccacc agctggagta actgttgaag taccagctaa cacacaagta attgttaaag
    52501 gtgcgaacaa agaagaagtt ggcgaattag ctgcaaacat tcgtggaact cgtcctccag
    52561 aaccttataa aggtaaaggt attcgttatg ttggcgaata tgtacgccgt aaagaaggta
    52621 aaactggtaa ataatcgttt agaaaaattg catagaagcc agcagcagga aaaaggcatt
    52681 tggctgaagt ttaccttcaa aaaaatacat ttttacttgt gccgcagctt ctatttttcg
    52741 aaacgtattt actgcttgaa atagtagtac aataaaaata aagaggtgac aattgtgatt
    52801 acaaaaccag ataagaacaa aacacgtcaa aagagacatc gtcgtgtacg taacaatatc
    52861 tctggtactg ctgagcgccc acgcttgaac gtttttcgtt ctaacaaaaa catctacgct
    52921 caaattattg atgacgtagc gggtgtaacg ctagcaagtg cctctacctt ggataaagaa
    52981 atttcaggtg gaacaaaaac agaagcagca caagctgtcg gtaaactagt tgctgaacgt
    53041 gcagctgaaa aaggcattaa agtagtagtc tttgaccgtg gtggatacct ttaccatggc
    53101 cgtgtgcaag ctttagctga agctgctcgc gaaaatggac tagaatttta gaagaaggag
    53161 gaataccatt catggtttat atcgatccaa aacatttgga attagaagac cgcgttgttg
    53221 caattaaccg tgtaacaaaa gttgttaaag gtggacgtcg tctacgtttt gctgctttag
    53281 ttgttgtcgg tgacaaaaac ggacacgtag ggtttggtac aggtaaagct caagaagttc
    53341 ctgaagcaat ccgtaaagca attgaagatg cgaagaaaaa cttagttgaa gtacctatgg
    53401 ttggttcaac aatcccacac gaagtaatcg gtgcttttag tggtggtaga atcctaatga
    53461 aacctgctgt agaaggttct ggggttgctg ctggtggacc agttcgtgcc gtattagaat
    53521 tagctggggt agcagatatc acttctaaat ctttaggctc aaatacacca atcaacgttg
    53581 ttcgcgcaac agttgaaggt ttgaaacaat tgaaacgtgc agaagaagtt gcagaacttc
    53641 gcggcaaatc agttgaagaa ttaatcggat aaggaggaca acaataatgg ctgaattaaa
    53701 agttacttta aaacgcagta ttatcggacg tcctcaaaac caacgcgata cagttaaagc
    53761 gttaggtctt acaaagatta acagttcagt ggttaagcct tctaatgaag caattaaagg
    53821 catgatcaat actgtttctc acctagtgga cgttgaagaa atttaattta gaaaaatcat
    53881 aactagaaca ttaaggaggt gccaaaccta tgaaacttca tgaattaaaa cctgctgaag
    53941 gttcacgcca agtacgtaac cgtgttggtc gtggtacttc atctggtaac ggaaaaactg
    54001 ccggacgcgg acaaaaagga caaaaagctc gttcaggcgg cggtgtgcgt ttaggatttg
    54061 aagggggaca aacaccattg ttccgtcgtt taccaaaacg aggattcaca aacgtgaacc
    54121 gtaaagacta tgcagtcatt aatcttgatg tattgaaccg ctttgaagac ggagctgaag
    54181 taactcctgt tgctttagtt gaagctggaa tcgtgaaaaa cgaaaaagct ggaatcaaag
    54241 tattagctaa tggggaacta aacaagaaat taactgtgaa agcagcaaaa ttctctaaag
    54301 cagctcaaga agcaattgaa gcggctggtg gatcaatcga ggtgatctaa tgtttagact
    54361 attaaaagat gcttttaaag taaaagacat tagatcgaaa atcctattta ctgtatttgt
    54421 gttgtttgta tttcgcctag gtgcacatat tactgtacca ggcgtaaatg ctgcttcatt
    54481 gcaaaacctt tcttctttgc catttttcaa catgttgaac ttggtaagtg gtagtgcgat
    54541 gcaaaacttt tccattttct caatgggggt ttcaccatac attactgctt ctatcgtcat
    54601 tcagcttctt caaatggaca tcgtgcctcg atttgttgaa tggtcgaaac aaggggaagt
    54661 tggtcgtaaa aaactgaatc aagcgactcg atatctgaca ttagtattgg catttatcca
    54721 atctgtcggt atcactgctg gattcaatca atacgctcaa ttaggtttcg tgaataatcc
    54781 taacgtagca acttacgtaa cgatcggttt gattctaaca gccggtacga tgtttgttac
    54841 ttggctagga gaacagatca cagaaaaagg tatcggaaat ggcgtatcaa tgatcatttt
    54901 tgcaggtatc atttctagat tgcctgaaag tatcagagaa atttatgaag attactttat
    54961 aaatattgat ccttcagaca tatggaaatc tgctattttt gtggccattt tactagtagc
    55021 ggtcttgttg atcgtgacat tcgtaacatt tttccaacaa gctgaacgca agatcccgat
    55081 ccaatatacg aaacgcgtag ctggcgcgcc tacaagcagc tatctgccgt tgaaagtaaa
    55141 tgcagctgga gttattccag ttatctttgc tagttcgttt attgctacgc caaatgctgt
    55201 tttacaagca ttgagcggta gattcggttc tgaaaactgg tacaaagtga tgatggagat
    55261 ctttaactat aatacagcac ctggtgctac gatttatacg attctgatcg ttgccttcac
    55321 tttcttctac gcctttgttc aagtaaaccc tgagaaatta gcggaaaact tgcaaaagca
    55381 aggaagttat ataccaagcg tacgccctgg taaggggact gaagagtatg tctcaagatt
    55441 actgatgcgt ttaagtacag ttggtgcagt cttccttggt ttagtggcat tgcttccaat
    55501 cattgctcaa atggtttggg atcttccaca atcaatcgga ttaggcggta caagcttact
    55561 gatcgttatc ggtgtagctt tagaaactgc gaaacagtta gaaggcttaa tgctgaaacg
    55621 taaatatgtt ggattcattg agtaagaaag cgtgttgagg tttcccctca acacacgttc
    55681 tcacacattt attaggaggg aaaacaatga acctcatttt aatggggctg ccaggcgcag
    55741 gtaaaggaac acaagctgaa aaaatcattg atgcttatgg tattccgcac atctcaacag
    55801 gagatatgtt ccgcgcagcg atgcaaaacg aaacagctct tggcctggaa gcaaagtctt
    55861 atatggataa aggtgcatta gttcctgatg aagtaacaaa tggaatcgtg aaagagcgac
    55921 tagctgaacc agatacagag aaaggcttct tattagatgg ctttccacgt acgctagatc
    55981 aagcaaaagc tctggacgca atgctaaaag atttgaataa aaaaattgat gctgtcatcg
    56041 atatccatgt tggtgaagaa atcttggtcg aacgtctagc cggccgattt atctgcagca
    56101 attgtggagc aacttaccat aaaattttca accctacaaa agttgaagac acatgtgatc
    56161 gttgcggtgg gcatgaattc tatcaaagag aagatgataa acctgagacg gttaaaaatc
    56221 gtctggctat caacatcaaa aatagtgaac caatcttggc ttattataaa gaacaaggtt
    56281 tgctaaacac tattgatggt gctcgtgaaa tcgatgctgt atttgcagat gtacaaaaaa
    56341 tcattgaaaa atagcacgat tgcccgtagc cgacttgcat tatgcagtta ctcttgtcaa
    56401 gttcaataaa ttatgataga atatgtcagt ccgacttcta gagaatcctc tagatgatcc
    56461 attttgagaa ttttgaggtg ggaactgcag tttccgccat tttatcgtgt gaaaatgcga
    56521 aacaagtaca aggaggtact gtgcgtggca aaagaagata tgattgaagt cgaaggtaca
    56581 gtcgtcgaaa ctttgccgaa tgcaatgttt aaagttgaac tagaaaacgg acaccaagtt
    56641 cttgctactg tttcaggtaa aattcgtatg cattacatcc gtatcttacc tggtgacaaa
    56701 gtaacggttg aattgtctcc atacgatctt aaccgtggcc gtattactta tcgctttaaa
    56761 taattgtact ccgtaaaact acaggaggta ttatcatgaa agtaagacca tcagtaaaac
    56821 caatgtgcga acattgtaaa gtaattcgtc gtaaaggacg cgttatggtg atttgcccag
    56881 caaatccaaa acataaacaa cgtcaaggat aattggaggt gctataaata tggctcgtat
    56941 tgcaggagta gatattcctc gtgataaacg tgtagtagtt tcacttactt atatctatgg
    57001 tattggtaac actactgcta aaaagatctt agctgatgct ggtgtatctg aagatgttcg
    57061 cgttcgtgaa ttaacgaatg aacaaacaga tgctatccgt gcagaagtag ataaattaaa
    57121 agttgaaggg gatcttcgtc gtgaagtgaa cttgaacatc aaacgtttga tggaaatcgg
    57181 ttcttaccgt ggaatccgtc atcgccgtgg attgcctgtt cgtggacaaa acacgaaaaa
    57241 caatgcacgt actcgtaaag gcccagctcg cgctatcgct ggcaagaaaa aataattctt
    57301 aagtgaagga ggttaaaact tcatggcagc aaaaaaagtg aatcgtaaac gccgtgtgaa
    57361 gaagaatata gaagcaggta ttgcacatat ccattctaca ttcaataaca ctatcatcat
    57421 gatcactgat actcacggaa atgcattagc gtggtcatca gctggttcat taggtttcaa
    57481 aggaagcaaa aaatcaactc cttttgccgc tcaaatggca gcagaaactg ctacaaaagc
    57541 agcaatggaa cacggattaa agactgttga agtaacagtt aaaggacctg gttctggacg
    57601 tgaagcagca attcgttcat tacaagctac aggtttagaa gtgactgcaa ttcgtgacgt
    57661 gactccagtt cctcataatg gtgcccgccc tccaaaacgc cgtcgtgttt aatgagtgcc
    57721 gctcattttg agttttatca gtaacgtcat tgaacaagac acttttcgtt ttgaaagggg
    57781 taaagataag aatgattgag ttcgaaaaac caagaatcgc aaaaattgat gaagaaaaag
    57841 attatggcaa gttcatcgtt gaacctcttg aaagagggta tgggactact ttagggaatt
    57901 ccctacgtcg tattttatta tcttctttgc caggtgctgc catcactaat attcaaatcg
    57961 atggtgtact gcatgaattc tctaccatca aaggtgttag agaagacgtc actcaaatca
    58021 tcttgaacat taaaggctta gcgctgaaaa tgtatggcca agaagaaaaa acccttgaaa
    58081 tcgatattac cggacctgct acagtaactg ctggcgatat tatcgttgat agtgatgtag
    58141 aaattcttaa caaagatatg tacatctgta gtgtctctga aggagctacg ttccatgctc
    58201 gcttaacagt gaaacctgga cgtggttatg ttcaagcaga tgaaaataaa aaagaagata
    58261 tgccaattgg cgttcttcca gtcgattcaa tctacacacc tgttcgtcgt gtgaactatc
    58321 aagttgagaa cacacgtgtt ggacaccgtg aagattttga taaattgacg atggagattt
    58381 ggacagatgg ttcaattgaa ccgttggaag caatgagttt gtctgcgaaa atcatgactg
    58441 aacatttgga tatttttgtg aacttaactg atgaagcaaa aaatgctgaa atcatggtcg
    58501 aaaaagaaga aacacaaaaa gaaaaaatgt tggaaatgac gatcgaagag ttagacttgt
    58561 ctgttcgctc ttacaattgt ttgaaacgtg caggaatcaa tactgtacaa gaacttacaa
    58621 ataaatctga gcctgaaatg atcaaagtcc gtaacttagg acgtaaatca ttagaagaag
    58681 ttaaagcaaa attgcacgac ttaggtttag gtttacgtaa agaagattaa aaccttttac
    58741 gaaggaggga aaccaacgtg agttatcgta aactaggacg cacatctagc caacgtaaag
    58801 cgatgttgcg tgatttaaca actgatttat taatcaacga acgtattgtg actactgaag
    58861 ctcgtgcgaa agaagttcgc tcaactgcag aaaaaatgat cactttaggt aaacgcggag
    58921 atttgcatgc acgtcgtcaa gctgcggctt tcgtacgtaa tgaagttgcc agcgtgcgtg
    58981 aagaaaatga ggacatcgtc attgaatcag ctttacaaaa actgttcaac gatattgcac
    59041 ctcgttacgc agaacgtcaa ggcggataca cacgcatttt gaagacagaa ccaagacgcg
    59101 gtgatggtgc accaatggtt gtcttagaac ttgtctaaaa tcacactaat aacatcactt
    59161 tatagatgat tcttttagag tgttatgatg ttggaagata tttttccgag tctagctcag
    59221 cgctgctcct caagcgaata tcgcttgggg agtaagcact catcataaag tgttttttta
    59281 taccttcaat tacctgtcat gacagatatg tttgaaggca taaaaaaaca ttttaagcga
    59341 gaaataattg aaaattttgt ctttttttga tagtgttagt aggaacttta ggatgaagaa
    59401 ggagtaatca tggagccaat tattgaatta gaaaagataa attataaata ccagccagat
    59461 gatcttcgcc ccgcattgaa agatgtctcc tttacaattg ataaaggtga gtggatcgct
    59521 attattggac acaatggttc aggaaaatcg acacttgcca aaacgatcaa tggattgctt
    59581 ctgccagaat cagggatcgt gaaagtggga aatcaaatat tggatgaaga aaatatttgg
    59641 acgatccgac agatggttgg aatggttttt caaaatccag acaatcagtt tgttggttcg
    59701 acagtagaag atgacgttgc ttttggtctt gaaaatcaag ggattcccag agaagaaatg
    59761 ctagtgcgtg tgaaagatgc acttgagaaa gttagaatgg ctgagtttgc ttcacgcgag
    59821 cctgctcgtt tatcaggagg gcaaaaacaa cgtgttgcca ttgccggcgt ggttgcctta
    59881 cgacctgata tcattatttt agatgaagca accagtatgc tagatccaga aggtcgtgaa
    59941 gaagtgatct ctacgatcaa gaagatcaaa gaagaaagtc aattgaccgt tatatctatc
    60001 actcatgata ttgatgaagc agctaatgcc aatcggatac tagtgatgag acaaggcgaa
    60061 cttgtccgtg aaggtacacc aaaagaaatt ttttctgctg gtcctgaact gatcgactta
    60121 ggtctagatc taccatttcc tgaaaaactg aagagcgcac tgaaagaacg tggggttgat
    60181 gtgcccagtg aatatatgac agaagaaagg atggtggatt ggttatggac atccgttttg
    60241 aacaagtaga cttcacttat cagccgaata caccatttga acaaagagcc ttatttgaca
    60301 tcaatatgac gatcaaagaa aacagttata ctgcattagt agggcatacc ggaagcggga
    60361 aatcaacctt acttcagcat ttgaatgcgc ttgttaagcc aacaagcggt acagtccata
    60421 ttggagaacg agatattcag ccggatacgg ataataagaa tttaaagccc atccgaaaaa
    60481 aagttggcat cgtctttcag tttccagaag cacagctatt cgaagaaacg gtagcaaaag
    60541 acattgcttt cggtcctaaa aactttggtg tcagcgaaga agaagcacta gtcctagcaa
    60601 aagaaacatt agaacaagtt gggctggatg aaagctattt ggaacgttcg ccatttgaac
    60661 tttcaggagg gcaaatgcgt agagtagcaa tcgctggtgt gcttgccatg aggccggaag
    60721 tacttgtatt ggatgaacca acggcaggac ttgatcccca gggtagaaaa gaaatgatgg
    60781 agatgttttg gcgtcttcat aaggaacagc agatcactat tgtattagtt acgcatttaa
    60841 tggatgatgt agccaatttt gcagattatg tctatgtgtt ggaaaaggga cgaatcgtta
    60901 atagcgggga gccgcaagag gtctttcaaa acattgaatg gctaaaagaa aaacaactag
    60961 gcgtacctac tgccaccgag tttgcagaag aattaatggc taaagggatg aactttgcta
    61021 ccttgccttt gacagctgaa gaattagcag acgctatcgt ccattatgct ggaggaaatg
    61081 cccatgatga ataaattgat atttggtcgt tatatgcctg gagattcctt gatccatcga
    61141 ttggatcccc gagctaaatt attagccagt ttttatttta tcggaatcat ttttttagca
    61201 aataattggg aaacgtacct tttgttggca tttttcacat tactttctgt cttcttgtca
    61261 aaagtagatt tgaaattttt tatccgaggc attcgcccat tgatctggct aatcctcttc
    61321 actgtagctt tgcaggtctt gtttacaagt ggcggagaaa cttattggag ttggggtgtt
    61381 ttccatatca ctgaatttgg tgtgatgaac ggcctcttta ttttctgtcg attcgtgttg
    61441 atcattttta tgtcgactct actcacgctt actacaccgc ctttggaatt atcggatgcg
    61501 attgagtact tgcttcgtcc attgaaagcc gtacggttcc cagtccacga aatttcattg
    61561 atgctgtcga ttgctcttcg ttttgtgcct acattgatgg acgaaacaga aaagatcatg
    61621 aacgctcagc gcgctcgcgg tgttgatttt ggtgaaggaa acctagttca gaaaatgaaa
    61681 gcagtcattc ctttgctgat tcctttattt gtcagcagct tcaaccgtgc ggaagatctt
    61741 gcgactgcta tggaagcaag aggctacaaa ggtggggaag gaagaacgaa atatcgtgta
    61801 ttgcattggc ataatagaga cacgatggtt atcattgctt ttgtattatt aactgtgcta
    61861 ctgatctttt tgagaggata aacgttcagt gggccgctat ctattctttt cttgctgatc
    61921 tgtttgcaag aaaggatgcg agcagtgtat gacaagaaat attagggtct gtggcagact
    61981 aaggtcacag gcctctttct tttatctgaa aagaaagaac gaaaaaattt tataaaaaaa
    62041 tcattattcc agataacctt ttaggaacaa aggagagaaa aacatggtac gttacaaagc
    62101 aatcattgca tacgacggaa caaattttaa tggattccaa aaacagccaa atgggcgtac
    62161 tgttcaagaa gaagtagaaa aaacattaca aaaaatggca aatggaaaag aaatcacggt
    62221 ctttggttcg ggaaggacag atgccggagt acatgcgatt gggcaagtaa tccattttga
    62281 ttatccagag gagcgtccgt tagaacgaat gcgtttcgcg ctagataccc agtcaccaga
    62341 ggatattgct gtaagacaag tagaaattgt ttcagaagac ttccacgcaa gatatttggt
    62401 caaagaaaag acgtatcaat ttcgagtgga tatagggaaa ccacgcagtc cttttcgacg
    62461 ccattatgca agttattttc cttaccctct tgatttggaa aaaattcagc gggcgcttcc
    62521 tgatttatta gggactcacg atttcacctc tttttgtgca tcaggaagct cgatagagga
    62581 taaagtacgt acgatctacg aggctaagat ggaggtaaat gaaacaaaag acgaattgtt
    62641 gtttacattt aggggaaatg gcttcttgta taagatgata cggatcttag taggaacgct
    62701 tttgaaaatt ggcaatggcc gattgcctga agatagtatc ccagatatta ttgcgaaaaa
    62761 agatcgtaat gcagcaggac ctacagcaca tcctgaagga ctttatttgt atgaagtgaa
    62821 atatgaataa gaggttgtga cagaagtggt cagcttcaag aaataagaag gaatttacga
    62881 aaattacttc tcaaaatttt gcaaaatttc ggcttatttt cgaaggagct acttctggaa
    62941 caccgtgaat cagaggttgt gaaaaagctg ccctgcatca agtaataaga acaaccaaac
    63001 aaaaatagct cgtcatattt ttcgtttggt tgggcttatt accgcagatg caagcttttg
    63061 aacaccgttt aaggaagagg ttgtgaaatc agcgttttga cctgagtatt agagaaacaa
    63121 acggaaaaat agttcctcat attttgccgt ttgttgagct aataccgaag gtcagctgat
    63181 tgaacaccgt ttaagaaaga ggtcgtgagc ctgtcattta actctgagtc acaagtaaca
    63241 atttcaaaaa acagcttctc atctttgatg aatatcgtga cttgtggacg aggcgttgat
    63301 aagtgaatca tctttttgtt acctattttt tctgtaacca aaaaacgatg gaacgttgtt
    63361 ttgattgccc ttaatgagga cagtcggaac aggtttgtca tttttatatt taaatggcac
    63421 aaatttttct aattatttgt catttagcgc tgaaaatttt tgctatttat ctaaaaaagt
    63481 gataactact taagatgaag cagaatattt acgccatacg gcatacaacc gcgaaaaata
    63541 taaaaaaaag aaaacgattg aacgcgtttt tgcagtcgca aaggaaaagc atggaatgcg
    63601 ctacatcaag tatcgagact tggaaaacaa tcaggtgtat acgctgattg tttttgctgc
    63661 aatgaatctg aaaaagttgt ccacttggca gttggaacat atgtcttcat atcttttatt
    63721 atacaaaaat agcaaaagca ccagtctaca ctcttagtaa agaggaaact gatccttttg
    63781 tctacattct taaagtactt aaactaagta ctttatttta ctctttagga ttgatacagt
    63841 agcatctatc cctttattaa taatctttta tttttttaaa taaagatgtt gtctccatct
    63901 tcaggcacta aaactttttc ggaaattcct ttttctttat tgaaagactt taattcttct
    63961 cttgacagtc cccaatggtt tacagcttcc atatgaacgg caataattgt ggaatctggt
    64021 attgctttat gcaattcata taaatcgtct ttccccataa taagagattc tccaactaaa
    64081 aaatgattat ctccagcatt tgctactatg atttctggat tatactgatc aatagtttgt
    64141 ttaacagctt cataccaaac tgtatctcct gtaaagtata gtgtcttctc agtcgaatgt
    64201 ttgaaaacaa taccgcaaac attacctaca taattcaaga tttctcctct gccatgtttg
    64261 ccttttgttt taaccaattg tatagattca aaaactgtgt cttctcgtag tacctccaca
    64321 tttttaaaac catatagtcg aatttcattc gcatcatttt catcttgcac aaataatttt
    64381 atttctttag gtaggacctc tttcgctata tcgtcccaat gatctagatg taaatgtgtt
    64441 acaataacag catcaatacc gttgataata tcttcgactg gcattggcaa atcaactaga
    64501 ggattcattt gatcttttct tggtgatgga aaaggacctt tcgaaataaa tggtggaagt
    64561 gttccttttt ccgctaacat tggatcaatt aaaaattttt tacctccata gtctaagata
    64621 gctgttgcat tacgaatgtg atgtatattc ataaacgttg tctccttttt gttgatatga
    64681 aatattttta gtataattca attccataca ataaataaac cgaacaaaaa aagtctttta
    64741 ctatttttac ttaatttatg aaaggttgtt taccatgtta gaccaaacag atataaatat
    64801 cattgaggaa ctatcaaaaa atagtcgaat cacaatgaaa gaacttggag aaaaagtaca
    64861 tttgactgga cccgctgtct cggcgagagt gacaaaacta gaggaaagtg gagttattga
    64921 aaattataca gtaaaattaa atatggaaaa gctagggttt tcgattcacg cttttttaat
    64981 tataattacg caaaatttaa atcataaacc ctatttatca tttattaaaa atcatgaaaa
    65041 attcttgatt agaaattata agattagtgg agggggctgt tatcttttgg aatgtagatt
    65101 tacttcaaaa aaatcaatga acagcttttt ggaacagctt aatgaatatg cgaattataa
    65161 attatctatt gttatagatt aataataaaa ctagcagatg gtaaaaatac taatatgcga
    65221 gacacacatt caaccataac tatataactt atcaatttaa aactaaactg atggaaacaa
    65281 gtaaggcaaa aacatttata ttttgcccta cttatttttt taattaaatt cattgatgat
    65341 tcatatttag aaaattctat gaaggccaaa tgagtatcct gtcacttttt tagataaata
    65401 gcaaaaattt tcagcgttaa atgacaaata attagaaata tttgtgccat ttaaatataa
    65461 aaatgacacc aagtatatca tgaacgcaca aaaagaccaa cagggtatac cctgttggtc
    65521 taaataa
//
LOCUS       DASHHB010000009        65328 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.009, whole genome shotgun sequence.
ACCESSION   DASHHB010000009 DASHHB010000000
VERSION     DASHHB010000009.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 65328)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 65328)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..65328
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.009"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(513..2009)
                     /gene="lysS"
                     /locus_tag="U2K71_000651"
     CDS             complement(513..2009)
                     /gene="lysS"
                     /locus_tag="U2K71_000651"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HEM7314759.1"
                     /translation="MTQEQQHQVEDLNDQMLVRRQKMEQLREDGIDPFGKRFERTHNS
                     EELHELFDPRTKEELAEMGLTASVAGRMMTRRGKGKAGFAHLQDREGQIQIYVRKDQV
                     GDEAYEVFKHADLGDFFGVTGQIMKTDTGEVSIKASEITILSKALRPLPDKYHGLTNI
                     EQRYRQRYLDLISNRESFDRFMKRSQIISEIRRYLDGNGYIEVETPVLHNEAGGAAAR
                     PFITHHNALDIDLYLRIALELHLKRLIVGGMEKVYEIGRVFRNEGIDTTHNPEFTMLE
                     AYTAYTDFNDVMNLTEGIIRNAAEKVLGTAKITYDGQAVDLESDFKRIHMVDAIKEQT
                     GVDFWQEMTLEEALALAEKHNVEVTEAMGVGHVINEFFETFVEETLTQPTFVYGHPVE
                     VSPLAKKNPEDPRFTDRFELFIVGREFANAFTELNDPIDQRERFEAQEKERELGNDEA
                     HGVDEDFLEALEYGMPPTGGLGIGIDRLVMLLTDAQSIRDVLLFPTMR"
     gene            complement(2120..3124)
                     /gene="dusB"
                     /locus_tag="U2K71_000652"
     CDS             complement(2120..3124)
                     /gene="dusB"
                     /locus_tag="U2K71_000652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HEM7314760.1"
                     /translation="MEHTWKIGNVEIPNRVVVAPMAGITNSAFRVTVKEFGAGLVVCE
                     MISDQGIHFRNKKTLEMLHIEETEHPLSLQIFGGNKDSLVEAAQFVEENTAADIIDIN
                     MGCPVNKVIKAEAGAKWLLDPEKVYEMVQAVSSAVNIPVTIKMRIGWDEEHVFAVENA
                     LAAQSAGASAIAMHGRTRVQMYEGKADWEVLKEVKKHLTIPFMGNGDVKTPEDAKWML
                     DYVGADGVMIGRAALGNPWMIHRTQHYLETGELIPEPTPAEKIATAKLHLQRLADLKG
                     EKVACREFRTHAAYYLKGIPRAAKVKVAINQTEKQQEINDLLDQFVEKAEKVLLKQTL
                     "
     gene            complement(3125..4012)
                     /gene="hslO"
                     /locus_tag="U2K71_000653"
     CDS             complement(3125..4012)
                     /gene="hslO"
                     /locus_tag="U2K71_000653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp33 family molecular chaperone HslO"
                     /protein_id="HEM7314761.1"
                     /translation="MKDYLVKALAHDGFVRAYAVQATNTVAEAQRRHDTWHTSSAALG
                     RTLVGSLLLGATLKGEDKLTVRIQGDGPATGIVVDANGKGDVKGYIKNPHLSLPLNSE
                     GKIDVRGAVGTQGMFTVTKDLGLKEPFSGQTPIVSGEIAEDFTYYLAISEQIPSAVGL
                     SVLVNTDDSIKTAGGFMIQIMPGADEETISKIEEQLKTTPRISTLLDEGQTPEEILKG
                     LLGTEELNILEKMPVQFKCDCSKEKFAEAIIALGAEEIQAMIDEDHGAEAVCSFCNNK
                     YKYSEEELDGLKKEALGGI"
     gene            complement(4229..6340)
                     /gene="ftsH"
                     /locus_tag="U2K71_000654"
     CDS             complement(4229..6340)
                     /gene="ftsH"
                     /locus_tag="U2K71_000654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HEM7314762.1"
                     /translation="MNKKNNGMMKNALYYVLVILAMVMVVYFIFGNNGQRSSDIEYSK
                     FTEQLQAGKIKEFEVQPANGVYKITGEYKEQQTVANSGGLAILGSTDTTTTHFTTIIL
                     PNDTTLSQVTELAKENKVATTVKEESSSGIWVSLLLSFLPIVIIIFFFYMMMGQQGGG
                     GGGGGRVMNFGKSKAKEADKKANRVRFSDVAGAEEEKQELVEVVEFLKDPRRFVELGA
                     RIPAGVLLEGPPGTGKTLLAKAVAGEAGVPFYSISGSDFVEMFVGVGASRVRDLFETA
                     KKNAPAIIFIDEIDAVGRQRGAGMGGGHDEREQTLNQLLVEMDGFDGNEGVIVIAATN
                     RSDVLDPALLRPGRFDRQILVGRPDVKGREAILRVHARNKPMADDVDLKVVAQQTPGF
                     AGADLENVLNEAALVAARRNKKKIDASDIDEAEDRVIAGPAKKDRVISKKEREMVAYH
                     EAGHTIVGLVLSRARVVHKVTIIPRGRAGGYMIALPKEDQFLMTKEDMFEQIVGLLGG
                     RTAEEIIFNVQSTGASNDFEQATALARSMVTEYGMSDRLGPVQYEGNHQVFVGRDYGQ
                     TKAYSEQVAFEIDQEVRKILMEAHDKAREIIEAHRAQHKLIAEKLLEFETLDAKAIKS
                     LFEHGVMPEGTESNDYPSEKEAKTFEEAKRALEEKDAEKQAEEKRDFEEAKHELNEEN
                     KAEHDSDSQQSEDNHSDDQYK"
     gene            complement(6430..6975)
                     /gene="hpt"
                     /locus_tag="U2K71_000655"
     CDS             complement(6430..6975)
                     /gene="hpt"
                     /locus_tag="U2K71_000655"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HEM7314763.1"
                     /translation="MLEKDIERILISQEEIQVRCKELGKELTEIYQNTNPLVVGVLKG
                     AVPFMADIVRSIDTYLELDFMDVSSYGNATVSSGEVKIVKDLDTNVEGRDLLIVEDII
                     DSGRTLAYLVDLFKYRKAKSVKIVTLLDKPEGRVVNIEADYVGFNVPNEFVVGYGLDY
                     AEAYRNLPYIGVLKPSVYQSN"
     gene            complement(6975..8420)
                     /gene="tilS"
                     /locus_tag="U2K71_000656"
     CDS             complement(6975..8420)
                     /gene="tilS"
                     /locus_tag="U2K71_000656"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HEM7314764.1"
                     /translation="MNVEKGGKKAPLLQQSFIRHGNEQGFWQQGSRILAAVSGGVDSM
                     VLLHLLLQVQRQIGFSLGIVHINHQLREESAEEAAYLRSYCEEKNLPLYVSIWEDPAK
                     SAIETEARNFRYQEFTRVMEEGQYDTLMTAHHGDDQIETVLMKIVRGGQLNTFSGIKE
                     VQPFATGRLIRPLLSFSKEDLYTYAAESQLVYFEDQTNQLLDIQRNRLRHLVVPQLKQ
                     ENTQALRHFQQFSQQIQWADQVIQKYMGQLIEKEVEQWEEQFRFSAEMIEEMTDAERY
                     YFFYSFFDKAFSKTGIILKEQQMESILDQWQQKKSQWQLSIGNNWILKREYHFVVLSK
                     KEKQATSQTEDQRLLLIPEQGIYLNETEWIGLVKPENAGDIKATDSWSLFSHDIWIPA
                     DVQLYVEKRKAGDRIKLTENLTKKVSRYFIDQKVPNSQRQHAWIITDAKRNIYSLIPF
                     AFSYLSIREETDKIHYILLYKYQKEAIGRRT"
     gene            complement(8540..9010)
                     /locus_tag="U2K71_000657"
     CDS             complement(8540..9010)
                     /locus_tag="U2K71_000657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein S1"
                     /protein_id="HEM7314765.1"
                     /translation="MSIEVGAKLQGKVSGITNFGAFIDLGAGKTGLVHISEVSNGYVK
                     DIHDVLSVGDEVTVKVISVNDDGKIGLSIRKAQEQPAESGEESRPKKYQQKDPRDNRN
                     RPAQKKSFSRSQPSAQKEDFDSLMSSFLKDSDDRLSSLKRNTEGKRGGRGGRRS"
     gene            complement(9056..9481)
                     /locus_tag="U2K71_000658"
     CDS             complement(9056..9481)
                     /locus_tag="U2K71_000658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HEM7314766.1"
                     /translation="MTKKSNKIAALDTEYAKKKYAEFQQQQRQLIFRRRRLAAIFVGA
                     LVIFAIIGVQIFHDMQRMHQLNELRVEANTEMKEVNADVDQLHQDVSLLKDDEYVAKL
                     ARSKFFYSKDGEKVYPILESNTDSSTNETDQSTSSTTAK"
     gene            complement(9548..9817)
                     /locus_tag="U2K71_000659"
     CDS             complement(9548..9817)
                     /locus_tag="U2K71_000659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding S4 domain-containing protein"
                     /protein_id="HEM7314767.1"
                     /translation="MRLDKFLKISRIIKRRSVAKEVADKGRIQINGKLAKSSSDVKVG
                     DILKVQFGNKTLEVKITELHESTKKEDAQKMYEIISENRTETLEK"
     gene            complement(9828..11423)
                     /locus_tag="U2K71_000660"
     CDS             complement(9828..11423)
                     /locus_tag="U2K71_000660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM7314768.1"
                     /translation="MDHKEMRRVMQGAFVLTIASFIAKVLSALYRIPLQNLVGDEGFY
                     VYQQVYPIYGIAMTLALSGLPQFISKYVAERKNPFERKQALQNLYPLVFWTGVFLWLF
                     VFAFSSWIAWMMGDIQLKPLIQVVSFTFLLMPGLSFYRGNFQGRFLMEPTAVSQVVEQ
                     FVRVFVIVCSAFAFRKLGWTVYQTGTAAMSGAVFGGLCAYGILHYYEQKIHGGALSFR
                     RFPLFKQPSKPLIRRFLVEGGLVSIYSGLLIFFQLIDSFFVKNALEVYGLSEYGAKIA
                     KGVYDRGQPLVQLGLVIATALSATFLPALTRHLTNRIYRQFLQTAKIYLRLTTALALA
                     ASLGLALLLPYINYALFKDYAGNAALVLFVFSIAFTAVIQAYQSIAQSKNSFRPSLKG
                     AGWGLLVKGLTTSFLTGLLGTAGASLSTLLGLGVTLWYFVRIETKELNAFWKERHFGR
                     KLMFSLLCMTVVLLLYYGVLNIVFGPVTHRSTAFFVSVIGVGIGASVFIYAVIRLRLF
                     TVREWLMLPFGKKILRLNKKIKK"
     gene            complement(11438..14959)
                     /gene="mfd"
                     /locus_tag="U2K71_000661"
     CDS             complement(11438..14959)
                     /gene="mfd"
                     /locus_tag="U2K71_000661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HEM7314769.1"
                     /translation="MDIIQLVGKDPQVKEWVSNLEEKMPRQLITGLAGSAKTLLFARA
                     FKKTNKNMIILMPNLYYANQLAEDLQHVIPQDQIHLFPVDEVLSAEMAFSSPEARGER
                     VATLNALQEKKAGIYLLPVASLHKRLPNKKTWLQAQLNWHIGDEIDVENLPRQLTLMG
                     YVREEMVAKPGEFSIRGSIIDIYPLTTTYPVRVELFDVEIDSMRYFEAETQRSIEKTE
                     EIWVSPTSEQVYGPEDLEHGIEQMQKMLEKRLAATTEEADREFLQDYFGQLTSSWQAG
                     VPTEEAKFYSDLLYQESVTILDYFPSNSLLVVDDYQRIMETNREIEREAAEWQTQKIS
                     ELRVFSEQTFSADIQKIVQKEKFTTTFFSLFQKGMGNLRFQAIHNFQYRTMQQFFGQM
                     PLLKTEMDRWRKQKQTVVIFIPTKDRIRKAEQMLRDEDILVVETEKNALIRGQVQLVE
                     GALQAGFELPQEKIVAITEKEIFQKTTKKQTRRQTVTNAERLKSYNELKAGDYVVHAN
                     HGIGKYIGMETLEVDGVHQDYMTILYQNDDKLFIPVTQLNLIQKYVASEAKAPRINKL
                     GGSEWTKTKRKVSSKIEDIADDLIKLYAARESEKGYAFGPDDAYQKEFENAFPYSETD
                     DQLRSAAEIKRDMEKEKPMDRLLVGDVGYGKTEVALRAAFKAIKESKQVAFLVPTTIL
                     AQQHYETMLERFEGFPVNIGLLSRFRTRKQQKETIEQLRTGQVDIVVGTHRILSKDIE
                     FSDLGLLIIDEEQRFGVKHKERLKQLRAQVDVLTLTATPIPRTLHMSMLGVRDLSVIE
                     TPPANRYPIQTYVMEKNPGAIREAIHREMGRGGQVFYLYNRVETIEQKVEEIQELVPE
                     ARIGYAHGQMTEAQLENTLFEFIEGQYDVLVTTTIIETGVDIPNANTLFVENADYMGL
                     STLYQLRGRVGRSSRVAYAYFMYEQQKILNEVSEKRLQAIKDFTELGSGFKIAMRDLS
                     IRGAGNLLGAQQHGFIDAVGFDMYSQMLSEAVSRKQGKNSQVEKTTVEIDLGVDAYLP
                     ETYVADQRQKIEIYKRIRELDSQEMLDELEDDLLDRFGEYPEEVAHLLTIGQIKMDGD
                     RALLETIRKQEAHIVFTLSKVGTKRYSVEQLFEALTATKLKASLGVEKEQMVIRLTIP
                     NQMEEAVWLQEIQKFVKALREQKYLSA"
     gene            complement(14976..15536)
                     /locus_tag="U2K71_000662"
     CDS             complement(14976..15536)
                     /locus_tag="U2K71_000662"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HEM7314770.1"
                     /translation="MKMIVGLGNPGTKYQYTKHNIGFMVVDKIAREHQATFKKNPFEA
                     EVAEFFHNGEKILLVKPQTFMNESGRAVGPLMTYFGIYPEELVVIYDDLDLAVGKIRL
                     RQKGSAGGHNGIKSIISHLNTNVFDRIKVGIGRPEGKKTVVQHVLSPFSKENQPLIEE
                     SMCQSVKAVEYLIEGHSFVDAMNRFN"
     gene            15889..16863
                     /locus_tag="U2K71_000663"
     CDS             15889..16863
                     /locus_tag="U2K71_000663"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEM7314771.1"
                     /translation="MTAAAEKKDHQKVILVGDGAVGSSYAFALVTQNIAQEVGIVDIN
                     TAKTEGDAIDLSHALAFTSPKKIYSATYADAHDADLVVITAGAPQKPGETRLDLVNKN
                     LKINRDVVTQIVESGFNGIFLVAANPVDILTYSTWKFSGFPKERVIGSGTSLDSARFR
                     QALAELVDVDARNVHAYILGEHGDSEFPVWSHANVAGLQIYEWVKNNPEIDEEAMVNL
                     FFGVRDAAYTIIEKKGATFYGIAVALARITRAILDDENAVLPLSVFMNGEYGLNDIYI
                     GAPAVINRQGIQKVIEIPLSDSEQDRMAASAKQLKEILDEAFAKLDAE"
     gene            17033..18433
                     /locus_tag="U2K71_000664"
     CDS             17033..18433
                     /locus_tag="U2K71_000664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase/peptidoglycan binding
                     protein"
                     /protein_id="HEM7314772.1"
                     /translation="MTRSSHRKKSKHVALLSVLGVLLVLISFYTYRSTYYTKHFLPNT
                     EINGINVSNLTVEKANEKLKEAYSDKKLAIKEDGKVWQEVPKSELGYKDDFTSELSPI
                     LNEQNGWTWGMTYVSAAEKQEIDPVSQNHQKLDTTVQTLTTKLTDLNKDRTPTTDATI
                     EKAGDSFVIKPEVNGNTIDVDAAVKQLKSAVNSGKDTIELTEFKEKPKVTSEDSSLKE
                     QLASMNAIANVKATYSINGETFQIPSSDIMSWLTYNDGKVDLDTEQVRQYVTDLGTKY
                     NTSTNDTKFKSTKRGEVTVPVGTYSWTIQTDSETEALKKAILAGQDFTRSPIVQGGTT
                     ADHPLIEDTYIEVDLENQHMWYYKDGKVALETDIVSGKPTTPTPAGVFYVWNKEEDAT
                     LKGTNDDGTPYESPVNYWMPIDWTGVGIHDSDWQPEYGGDLWKTRGSHGCINTPPSVM
                     KELFGMVEKGTPVLVF"
     gene            complement(18511..19233)
                     /locus_tag="U2K71_000665"
     CDS             complement(18511..19233)
                     /locus_tag="U2K71_000665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7314773.1"
                     /translation="MSGHSKWHNIQGRKNAQDAKRGKVFQKLSREIYMAAKAGGPDPS
                     MNPALRLVVDKAKAANMPNDNVERAIKKATSAVDETNYDEITYEGYGPGGVGILVHAL
                     TDNRNRTATNVRVAFTRNGGSLGETGSVSYLFDRKGYIAIERDGLTVDEETMFEDVLE
                     AGAEDLQTSPEVFEIYTAPEDFAAVRDELEKEFTLAQAELTMIPQTTIDLNEEQKEQL
                     ERLVDKLEDDDDVSEVFTAAEM"
     gene            complement(19312..19818)
                     /locus_tag="U2K71_000666"
     CDS             complement(19312..19818)
                     /locus_tag="U2K71_000666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HEM7314774.1"
                     /translation="MNSSKKQMKNGNIYLVIALIVVIILFYSSSQTYGQQSQVSRLES
                     WLPNQPFKDTLAGIRFNYGGSMVSINHLGYAKFLEFFLRKGAHFGTYFILGGSLFLGV
                     FPKLKIWWLTAILAWLSATGYAGLDEFHQMMTGGRTPMFQDVMLDSIGALTAVILCVL
                     FAAFKKRL"
     gene            19941..21740
                     /locus_tag="U2K71_000667"
     CDS             19941..21740
                     /locus_tag="U2K71_000667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HEM7314775.1"
                     /translation="MSYSLNWNLDSIFSGGSHSDALNQRMKQLEDQMNEYYHRVTKWS
                     PSSDNAEQLNAILQLQETITNGFTQCSSYITALLSANVNDSDAKVLSGKLYAMLPRLQ
                     SAETVLSKKFAEISDNDWNQMLSQSSFETIAFRLNEIRRDGSQLLSEAEENIINTLSL
                     DGLNAWSSHYDTIVASISIPFEQDGQVVELSAGQAFNKMMGDPDPKVRETLFAAWEEA
                     WKEKAPLFTDTLNHLDGFRLSDYELHGVTDFLQKPLEYNRLKKETLSVMWDTIQKNKQ
                     PLVNYLTRKANLFGKEKMEWQDQDAPIILGDLKEKTFSFDEAAAFIIENFNKFSPKMA
                     KFAQSAFEKSWIEAEDRPGKRPGGYCTELPETKESRIFMTYSNSVNEVATLAHELGHA
                     FHSSTMWDLPSLNREYAMNVAETASTFAELIVADATLKVAKTKEEKINLLDTKLQNAL
                     AMFMNIHSRFIFENRFYEARQEGLVSEDKITEMMVEAQKEGYQGALATYHPYFWAAKL
                     HFFIDDVPFYNFPYTFGYLFSLGIYAYANQKGANFEEEYIHLLRDTASMTTEDLAKKH
                     LNVDLTQPDFWQAGIDMVTEDIQTFMELTEEFI"
     gene            complement(21808..23382)
                     /locus_tag="U2K71_000668"
     CDS             complement(21808..23382)
                     /locus_tag="U2K71_000668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEM7314776.1"
                     /translation="MKKSLISAVMVCSMTLTAVASPIAAAADDFDSQIQQQDQKIADL
                     KNQQADAQSQIDALESQVSEINTQAQDLLAKQDTLRQESAQLVKDIADLQERIEKRED
                     TIQKQAREAQVSNTSSNYIDAVLNADSLADAIGRVQAMTTMVKANNDLMEQQKQDKKA
                     VEDKKAENDAKLKELAENQAALESQKGDLLSKQADLNVLKTSLAAEQATAEDKKADLN
                     RQKAEAEAEQARIREQQRLAEQARQQAAQEKAEKEAREQAEAEAQATQASSTAQSSAT
                     EESSATQSSMTEESSSATQSSATEESTTPESSTEESTAPESSATEESTTAPESSATEE
                     STTVPESSATEESTTVPESSTTEESTTPAPTTPSTDQSVDTGNGTGSSTPAPTPTPTP
                     EQPKPVTPAPAPSGSVNGAAIVAEAYKYIGTPYVWGGKDPSGFDCSGFTRYVYMQVTG
                     RDIGGWTVPQESAGTKISVSQAKAGDLLFWGSQGGTYHVAIALGGGQYIHAPQPGESV
                     KVGSVQWFAPDFAVSM"
     gene            complement(23637..24146)
                     /gene="mreD"
                     /locus_tag="U2K71_000669"
     CDS             complement(23637..24146)
                     /gene="mreD"
                     /locus_tag="U2K71_000669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HEM7314777.1"
                     /translation="MLKKETMKYYLPIVLFFLMLIDGHLTRMLGEWSKGTYMSNAHFL
                     ILALLCCSMAFEKRYLLITTIVLGAIYDAYYIGVIGIYAVALPLIVWLMYVMKDVIHV
                     NIFTEFFSMIIFVTGYELFTMVVQLIFKLAVVNNTYFITRFLGPTLLLNMIIFVLFIF
                     PFKKLFSDE"
     gene            complement(24150..25004)
                     /gene="mreC"
                     /locus_tag="U2K71_000670"
     CDS             complement(24150..25004)
                     /gene="mreC"
                     /locus_tag="U2K71_000670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HEM7314778.1"
                     /translation="MKKFNPNKNIIITLVIVIIVVTILSVTAAQRANEGKPNFFQSIV
                     NDSVAMVDKTISAPVRWVRNGVDSVHNLFSTYAENERLKEKIDSYDDVSQQNKNYERE
                     IEALKKELELNQTLTSYEKVTANVITRSPDSWQDMLIVDKGSKDGIEANMAVMSQKGL
                     IGRVIEVNTASSKIELLSSSNESSNHFPVRVSSANGEAFGLLKNYDEKLHALVVTQLT
                     GDTDIKEGDVVQTSGLGGNSPANLPIGTVIKTKPDSYGLDREVYVKPYAEMYDVSVVT
                     IVQRLAED"
     gene            complement(25159..25797)
                     /locus_tag="U2K71_000671"
     CDS             complement(25159..25797)
                     /locus_tag="U2K71_000671"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HEM7314779.1"
                     /translation="MKILLAAGGNPQNWPQSLLSKYDAYVGIDRGALYLLQAGYPVDL
                     AVGDFDSLSNEEQKEVFAKAKEYTRSPAEKDDTDTQLGILKTFERYPQAEVTLIGATG
                     GRLDHLLANLWLGMEPRFKPFAHQLIIRDCQNYLSYYLPGEHIVRQIPDMKYLAYCCL
                     TPVSNLTLKGSKYVLENKEVEYPTSYASNEFLSDTAEFSFSDGMIAVIQSKD"
     gene            complement(25798..26448)
                     /locus_tag="U2K71_000672"
     CDS             complement(25798..26448)
                     /locus_tag="U2K71_000672"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HEM7314780.1"
                     /translation="MKIAPSILSADFANLQRDIELVEKGGADYIHVDVMDGRFVPNIT
                     LGPNIVQAIRPVTKLPLDVHLMIVDPENYIPAFAKAGADIITVHVEATPHIHRALQMT
                     KSLGVKAGVVINPGTPVSMIKHVLPLADQVLVMTVNPGFGGQSFIEETVEKITELADL
                     RRENNWTYAIEVDGGIVPETAKICKDAGADVFVAGSYIYGAENPVGQIERLKEALD"
     gene            complement(26461..27360)
                     /gene="rsgA"
                     /locus_tag="U2K71_000673"
     CDS             complement(26461..27360)
                     /gene="rsgA"
                     /locus_tag="U2K71_000673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HEM7314781.1"
                     /translation="MALIKGQIRKAISGFYYVYANGETYQTRGRGNFRNRKITPLVGD
                     QVIFESENQTDGYLLEILPRKNQLVRPPVTNVDLGIIVTSIVEPAFSYNLLDRFLVTL
                     EYESIEPIIFLTKTDLSDDPEKIEEIKQTYTKIGYPVIVPNHTGDTTELIRYFPERLS
                     VFMGQSGAGKSTLLNQISPELNLATGEISDALGRGRHTTRHVELIPIYGGLVADTPGF
                     SSIDFLTIEAGELPRQFPEFVEASVHCKFRECMHAKEPGCEVKHRVETGGIAQTRYDN
                     YLQFLQEIEKRKPMYAKKEGKRK"
     gene            complement(27525..29594)
                     /gene="pknB"
                     /locus_tag="U2K71_000674"
     CDS             complement(27525..29594)
                     /gene="pknB"
                     /locus_tag="U2K71_000674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stk1 family PASTA domain-containing Ser/Thr
                     kinase"
                     /protein_id="HEM7314782.1"
                     /translation="MIELGKKLNGRYQITGNIGSGGMANVFLAHDLILDRDVAVKVLR
                     FDFQNDQTAIRRFQREALAATELVHPNIVSVYDVGEEDNMQYLVMEYVKGMDLKRYIQ
                     THYPVPYETAVNIMQQILSAISLAHNHQIIHRDLKPQNVLIDNEGVVKITDFGIAIAL
                     SETSITQTNTMLGSVHYLSPEQARGSMATKQSDIYALGIILYEMLTGSVPFDGESAVT
                     IALKHFQDDLPSIKALDPNVPQALENVILRATAKEPADRYKSAEEMSDDLSTVLSPAR
                     ANEEKWQPHVMDNETKVITPLTEDAPMPDSFKSMPLPKDKPENETLPEEEEVLPDPKK
                     KGQKKWWLILLAILAVLGIGTAVFFASGGRGEVVVPDVSDLSEASARETLAKAKLEVA
                     TKTEEIADDKIEEGNVVKTDPAAGTTVKQKREVTLYISSGTKKIKLDDYSGKSYEDAE
                     ASLKELGFSADLIQKEEEFSSEVESGMIISQSPSEDTEVDPKTDTITFTVSKGPETVT
                     LSSHVGENYETVRNWLVTQGFNINNISATYDYSDTVPSGSIISQDPASGEVVAEETYI
                     NFVVSQGTEPKLNDISGYTKSEAQSYLASVGAEYIGHETSEYSNTVDKDKVIRTVPGA
                     GTTITKGMVVNVIYSKGPDPASSSSEKDSSDSSSSSSSSSSTSNSSKEESSSSTSSSA
                     STSESSK"
     gene            complement(29591..30331)
                     /locus_tag="U2K71_000675"
     CDS             complement(29591..30331)
                     /locus_tag="U2K71_000675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Stp1/IreP family PP2C-type Ser/Thr phosphatase"
                     /protein_id="HEM7314783.1"
                     /translation="MQIEYQSDVGRRRNTNQDYASVFTSRSGLKLAVLADGMGGHRAG
                     DIASQMAVTNLGEAWEKQEAVDDEKIAQWFIQSIQEENMLIYQRGQEQPEYNGMGTTI
                     VAAALSDDWFTIANVGDSRAYLIREDKIMQLTEDHSLVNELVKSGEISEEMAVNHPRK
                     NILTRSVGMPGTVEVDVSTHLWQLKDRLLLCSDGLTNMLSEEEIGTIINQEGPLSHKV
                     TELIDRANEAGGADNITVLLIEYKEDSV"
     gene            complement(30344..31702)
                     /gene="rsmB"
                     /locus_tag="U2K71_000676"
     CDS             complement(30344..31702)
                     /gene="rsmB"
                     /locus_tag="U2K71_000676"
                     /EC_number="2.1.1.176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(967)-C(5))-methyltransferase
                     RsmB"
                     /protein_id="HEM7314784.1"
                     /translation="MKKIPKKMKKSVRYMALLTLERVNKGGAYSNLLLNEMIKQSELN
                     QKDVGLFTELIYGTISHQRLLEFYVAPLIAKAKKVDDWVKMLLYLSVYQLEFLDKVPA
                     HAILNEAVEIAKVKGNPGTGKFVNGVLRNYQRQGAPDLQGIADPIERLSVKISLPLWM
                     TKRLVDQIGYEETEKLGLSLYQPSHASARVDTRRLSRDQAITVLQEEEITAEKSQVSP
                     YGIVAKKGHLASSSLFHDGVMTIQDESSMLVAPSMQIEPEHHVLDACAAPGGKTTHIA
                     SFLDPSCGGEVVALDVHEHKVKLIEDNAARLGVSSAVYAQKMDAREVSKQFEAEQFDR
                     ILVDAPCSGLGLLRRKPDIRYKKQPDELANLPKIQLEILESCAPTLKSSGILTYSTCT
                     ILKEENQDVVSAFLERHPDFERIDVLTNPMLQSSIEEKMLTLYPHQFYTDGFFICCLR
                     KK"
     gene            complement(31702..32640)
                     /locus_tag="U2K71_000677"
     CDS             complement(31702..32640)
                     /locus_tag="U2K71_000677"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HEM7314785.1"
                     /translation="MTKLVFMGTPAFSVPILEGLIESGYEIQAVVTQPDRPVGRKRVI
                     TPTPVKKAALKHGIRVLQPEKISGSPEMEEIIELVPDVIVTAAFGQFLPEKLLQVPKL
                     GAINVHASLLPKYRGGAPVHYAIMNGEKETGVTIMEMIKKMDAGGIYAQESIPITKQD
                     DVGTMFEKLSLLGRKLLLETLPNILDGQKPVPQDESEATFSPNITREQEAIDWHKTAE
                     EIDNQVRGMRPWPIAFTTYEGIRWKLLSVEVLAETTDKAPGTIIRKDKKNLWIACGKQ
                     TVLAINELQPAGKGKQAVHDFLNGSGQQVQIGQQVK"
     gene            complement(32633..33124)
                     /gene="def"
                     /locus_tag="U2K71_000678"
     CDS             complement(32633..33124)
                     /gene="def"
                     /locus_tag="U2K71_000678"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEM7314786.1"
                     /translation="MRYPILIHPNDKLKRTAQPIDVITDETIALLDNLYETMIANDGI
                     GIAAPQVGQNKRIAVIEVDEGEKFELINPEIIEAKGESLDVEGCLSIPHVYGTVKRAD
                     EVTVRYYDRDGEEIEVTAFGYLARAFQHEIDHLDGILFIEKMIQQIPEEELEEYMEEH
                     LDD"
     gene            complement(33139..35547)
                     /gene="priA"
                     /locus_tag="U2K71_000679"
     CDS             complement(33139..35547)
                     /gene="priA"
                     /locus_tag="U2K71_000679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein N'"
                     /protein_id="HEM7314787.1"
                     /translation="MTLTAEVIVDVPTMQTDQPFTYLVPSEMETALQVGMRVEVPFGN
                     GNRHVQGFVMAIQKSEESANPSLKAVIRLLDLAPVVNEELLSLADYMKKITYAFKITC
                     LQTMLPSVMKAEYDKLIYPLADTPEVQVLFDQREVISWKEAEEEGSLSQLIRWRQEQL
                     VDIKYEVHTRNKVKTIRLVRSLLTEKQIEEEWAKLRQNAKKQKELLLCLSELSQEEPI
                     AYFKNKGISTAVLNQGKEKGWLEFIESERYRDPYKDRVFDQTTALELNAEQKNAVEQI
                     ITAGQQQKDQVFLLEGITGSGKTEVYLQAIADVLSENKTAIMLVPEIALTPQMVERFK
                     GRFGESVAVLHSGLSQGEKYDEWRKIEREEAQVVVGARSAIFAPLKNIGLIIIDEEHE
                     SSYKQDETPRYHARDLAIWRSKYHHCPIVLGSATPSLESRARAQKGVYQLLQLNHRAK
                     AAAQLPAIEIVDMREEFQNHRTSTFSANLQEKIQNRLDKKEQTVLLLNRRGYSSFVMC
                     RDCGFVLPCPNCDISLTLHMDTRSMRCHYCGHEEPIPNRCPNCGGNKIRYYGTGTQKV
                     EEELRELYPQARILRMDVDTTRRKGAHEQILQKFGAKEADILLGTQMIAKGLDFPEVT
                     LVGVLNADTSLNLPDFRSSEHTFQLLTQVSGRAGRAEKAGEVVIQTFNPQHYAIELAK
                     KQNYEQFYQQEMHVRHRGGYPPYYFTVKITASHPEEQVAAKKIFQIANQLKEVLSPQS
                     LLLGPTPSMILRVKNRYYYQLIIKYKHEPNLPHVLDEILNGSQKEQRQGLFVAIDNEP
                     LNFI"
     gene            complement(35800..36105)
                     /locus_tag="U2K71_000680"
     CDS             complement(35800..36105)
                     /locus_tag="U2K71_000680"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEM7314788.1"
                     /translation="MMLKPSIDSLLDRVNSKYSLVILASKRAHELDASAQPTLDSFDS
                     VKSVGQALEEIDAGNVVNDPHPELKRERLKMEEEERQAQKEREQHELESRIREEQKM"
     gene            complement(36110..36724)
                     /gene="gmk"
                     /locus_tag="U2K71_000681"
     CDS             complement(36110..36724)
                     /gene="gmk"
                     /locus_tag="U2K71_000681"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010766465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HEM7314789.1"
                     /translation="MSERGLLIVLSGPSGVGKGTVRKAIFESEDNDFQYSISMTTRKM
                     REGEVEGVDYFFRSKEEFESMIEAGEMLEYAEYVGNYYGTPLSYVNKTLDEGKDVFLE
                     IEVQGAKQVKEKVPDGVFIFLTPPDLAELKSRIVGRGTDADEVIEKRMKVAKEEIEMM
                     ALYDYAVVNDEVPLAVERIKNIIASEHFRVDRVIGKYIKMLKEM"
     gene            complement(37211..37495)
                     /locus_tag="U2K71_000682"
     CDS             complement(37211..37495)
                     /locus_tag="U2K71_000682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314790.1"
                     /translation="MKYYDVTFHELSGKTVVKRDIPSEKNGFDVWKDACADYNENELF
                     ILINDGAYVTMNRKFIVRIDTEEVEDPTEKARSRKDEIMGVVNTLSNMGF"
     gene            complement(37513..38403)
                     /locus_tag="U2K71_000683"
     CDS             complement(37513..38403)
                     /locus_tag="U2K71_000683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YicC family protein"
                     /protein_id="HEM7314791.1"
                     /translation="MKSMTGFGKAIRETKAYQLDIEIKSVNQRFLDVQIRSPKILNFV
                     ENDFRQLIKQQLSRGRIEVFVNLAYIGKNEKQIVINWELIDSLITQLTDGIQQRYGSQ
                     NQVSISRMAERFVLDENFVTVEEKPEESMEELAQLALDTLKSALDSIEASREKEGQAL
                     ALVLKKNTEEFKEVLKKLDSFVEIYEKEFQIRYQKKLEEFLGAKVDEQRLLTELAILL
                     ERGDIHEELDRLAIHVQKLDELIEAQCPVGRELDFLIQEMNREINTIGSKSNAIEIKN
                     QVVQSKTILEKIREQVQNIE"
     gene            complement(38423..38641)
                     /locus_tag="U2K71_000684"
     CDS             complement(38423..38641)
                     /locus_tag="U2K71_000684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdbC family protein"
                     /protein_id="HEM7314792.1"
                     /translation="MAQEFSYEIVEEIAILSENNKGWRKELNLVSWNGRPPKFDLRDW
                     APDHEKMGKGLTLTNEEFEQLQKAIENM"
     gene            complement(38611..40311)
                     /locus_tag="U2K71_000685"
     CDS             complement(38611..40311)
                     /locus_tag="U2K71_000685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HEM7314793.1"
                     /translation="MEIKKVYFSQKMSLYSPIQQAKQPLPFFQSHQESRDAFWRSAKK
                     QCAFKAVSKKYTVYFTIAKSRGDVIFNNLLIEGETFEWKKFFRYMEACARFFIKENCC
                     FLFQSPLSEEWLRIFHKNGYQGTTQLNKKLVYHTALVLGGGGAHGAYQIGVWQALKEH
                     DINFEIITGTSVGALNGALILQGDMKKAVNLWKKLSTRQVLALPEMAAVEDLRERFYR
                     ERRQMTKTALIEGGVSSAPLEKMVKDNLDLSLLKHNPKIRLYTVSTKLPELAEVVTDI
                     QQTKTEEIPDWILASASFYPAMAYRKIGKNKYADGGYRNKIPIDIAINKGATEAFVVD
                     VQGPGPAKKIRMPDIFIHWKCQTLWTLGSFLLFDSQRNQLNLQLGYLEMKKRLGYYYG
                     NWYTFHSVKPARTYWRGFLSYLTKEIQLELSFFKSIKFWQKLRNLYKNRVVPETCGLA
                     MLELLAKKHFLLPNEIYQVDMMIQMICQQDIKSMPDDLLAQIGQLSTEEWRRYRKYQQ
                     KIQNERTKTVYFDYLLQGKRKDRLQHELLKQPVDTLLILYLYYLKEEQPWHKNFHMKS
                     "
     gene            40578..41351
                     /locus_tag="U2K71_000686"
     CDS             40578..41351
                     /locus_tag="U2K71_000686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314794.1"
                     /translation="MTESVYVHIDTTSNAVLTKGLTATDFANSIVHFPQNLLLLDPSA
                     SAGEYESHTGLKVIRGTENIQRFFSSSRNRRGFDELKWIDFTDLTMLKELTPLEISEL
                     LYFGHMKTHLHSPFFYKLQNNFVYFDLNDQLNRIYYRYLDEFYRIFASKLSSILSDKI
                     NVKKSFFKKETPVEKISVELLKEMRNLMQEGIIFSFDQVGLIDGKYRIPIYVIEDYIW
                     KLKGTRYREEEIIATLIYSVTDRHWQLVEDADLSYLPLK"
     gene            complement(41432..42001)
                     /gene="thiT"
                     /locus_tag="U2K71_000687"
     CDS             complement(41432..42001)
                     /gene="thiT"
                     /locus_tag="U2K71_000687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupled thiamine transporter ThiT"
                     /protein_id="HEM7314795.1"
                     /translation="MGKNRVWVEGTIVAALAMVLSLIPIQIGSSFSISLGQIPLTLFA
                     LRRGTKAGMLAGFIWGILHFPLGQVYYLSVVQVLIEYPIAYTFAGAAGLMASNVQKGY
                     VDDNTKQIRKSIVFGALIGALSRYFWHFIAGVVFWGAYALWGMNPWLFSFVMNGASGL
                     STAIVTIIVLLGIQQTTPSIFVPDKRQEL"
     regulatory      complement(42067..42169)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(42175..43146)
                     /locus_tag="U2K71_000688"
     CDS             complement(42175..43146)
                     /locus_tag="U2K71_000688"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016618611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HEM7314796.1"
                     /translation="MSKHYFDPRLKIFALNSNRPLAEKIAAAVGVELGKSSVTQFSDG
                     EIQVNIEESIRGSHVYVIQSTSSPVNDNLMELLIMIDALKRASAKTINVVMPYYGYAR
                     QDRKARAREPITAKLVANMIQKAGATRMLTLDLHAVQIQGFFDIPVDHLMGAPLIANY
                     FLEKGIKGDDVVVVSPDHGGVTRARKLAEFLKSPIAIIDKRRPKANVAEVMNIIGQVK
                     GKTCVLIDDMIDTAGTITLAANALKEAGATSVYASCTHPVLSGPALQRIEDSAIERLV
                     VTDSIYLPEDRKIDKIDEVSVGGLMGDAIKRIHENKPVSPLFETKNK"
     gene            complement(43240..44613)
                     /gene="glmU"
                     /locus_tag="U2K71_000689"
     CDS             complement(43240..44613)
                     /gene="glmU"
                     /locus_tag="U2K71_000689"
                     /EC_number="2.3.1.157"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HEM7314797.1"
                     /translation="MDARYAIILAAGKGTRMKSKLYKVLHPVSGKPMVEHIINRVSET
                     KPDEVITIVGHGAEQVKAQLGERSKYALQAEQLGTGHAVLQAASFLEGKKGTTLVISG
                     DTPLLTTETLNNLFEYHQGKNASATILTAQAENPTGYGRIIRDHIGIVEKIVEQKDAT
                     PEEALVQEINTGTYCFDNEALFDALSKVGTNNAQGEYYLTDIIEILKEEGHTVAAYQT
                     DDFEESMGVNDRIALAKANEIMRKRINQMHMVNGVSFVDSATTYIDAGVEIGPDTLIE
                     AGVQIQGNTVIGSDCVIGSHSKIVDSRIEDHVVIENSVIESSHVKKHADVGPYAHLRP
                     KAEIGENVHIGNFVEVKNAQIGKGTKVGHLTYVGDATLGEEINVGCGVVFVNYDGKNK
                     HHTTVGDHSFIGSSTNIIGPVEVAKNSSIAAGSTITDNIPEYALAIARARQVNKEGYA
                     KKLPYLN"
     gene            complement(45116..45934)
                     /gene="purR"
                     /locus_tag="U2K71_000690"
     CDS             complement(45116..45934)
                     /gene="purR"
                     /locus_tag="U2K71_000690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pur operon repressor"
                     /protein_id="HEM7314798.1"
                     /translation="MKVRRSERLVDMTQYLLNHPHELISLTSFAERYESAKSSISEDL
                     GIIKKTFKERGYGTLETIPGAAGGVLFIPEISYEEAKKYIESLAERLSEQDRLLPGGY
                     VYLSDLLGEPELLRQVGRIIASKYLGEKIDAVMTVATKGVPIAQAVSYYLNAPFVIVR
                     RDSKITEGSTVSVNYVSGSSERIEKMELSKRSLKRGSRVLVVDDFMKGGGTVNGMKSL
                     IEEFESELVGITVFAESKFNGHRAIDDYTSLLYVKDVDTQTKNITVVPGNYFSE"
     gene            46140..47099
                     /locus_tag="U2K71_000691"
     CDS             46140..47099
                     /locus_tag="U2K71_000691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM7314799.1"
                     /translation="MTYNHLTPTELVMIEAYFNQSQPVSKVANLLQRSRQTIYKVYRF
                     LKSGGSAMSYYKQYKKNKRNCGRHPIVLPDEQQEYIQKKVVQGWAPDVLIGRAEFPIA
                     CSVRTLYRMFKDKTFDTHDLPMKGKRKPNGHKEKRGKQGFRRSIRDRKTDYTQFDHEF
                     GHLEGDTIVGGKHKSAVITLVERLSKVIITLKPEGRQAKDIENRLNQWFRCVPKNLFK
                     SITFDCGKEFSNWKKISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNG
                     VSETFIQSVASKRNHIPRKSLHYRTPMEVFLSYVTKDDLSNLI"
     gene            complement(47167..47991)
                     /locus_tag="U2K71_000692"
     CDS             complement(47167..47991)
                     /locus_tag="U2K71_000692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HEM7314800.1"
                     /translation="MALLSYEFMRRAFLAAIFIAGIAPMLGVFLVIRRQSLMADTLSH
                     VSLAGVALGFFLNLNPTLTTMLVVVLAAVILEYLRTIYRTYSEISIAILMAGGLALAL
                     VLMNLSGGNSATSIQSYLFGSIVTITWEQVIFLGILFVAIGILFFLFKRPMYVLTFDE
                     DTAHVDGLPVHLMSMMFNVITGVAIAVMIPIAGALLISAIMVLPAAIGMRLGKSFNVV
                     IAISVCVGFLGMLSGLTSSFYLDTPPGATITLVFIALFLIVNIIKRILVAFRRKNN"
     gene            complement(47964..48644)
                     /locus_tag="U2K71_000693"
     CDS             complement(47964..48644)
                     /locus_tag="U2K71_000693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HEM7314801.1"
                     /translation="MRYIEISDLTFYYDDEPVLEDVSYHVDAGEFVILTGENGAAKST
                     LVKASLGLLKPSKGMIKIAEKNQEGKKLSIGYIPQQVASFNAGFPSTVIELVRSGRYP
                     RGRWFKRLSEKDHQHVQRALEAVGMWDMRNRRIGELSGGQKQRISLARIFATDPDLFV
                     LDEPTTGMDEESRNEFYRLLRHSAHVHGKSVLMITHDHEDIKQYADRQIRLVRKEDSQ
                     WRCFHMNS"
     gene            complement(48667..49605)
                     /locus_tag="U2K71_000694"
     CDS             complement(48667..49605)
                     /locus_tag="U2K71_000694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HEM7314802.1"
                     /translation="MKKYLLTATVMIGALLFAACGNTNKEADKKEDLTIVTTFYPMYD
                     FTKEIVGDEGNVKLLIPAGTEPHDFEPSAKERAEISDADVFVYNSSDMEFFVDSLKDS
                     VDSKQTLMIEAAKGIDRLESQEADEHEESEEGHGHSHEYDPHVWLDPVLAIKEVRTIA
                     EELGEKYPDKKETFTKNADAYIKKLEALDQKYSDELKDATNRTFVTQHAAFAYLANQY
                     DLKQVAISGVSPDQEPTPSRLAELKEFVKKNNIKVIYFEENASSKVAETLSNETGVKL
                     EVLNPLESLTNEQIKAGENYISVMEKNLKALKESIN"
     gene            complement(49850..50713)
                     /locus_tag="U2K71_000695"
     CDS             complement(49850..50713)
                     /locus_tag="U2K71_000695"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HEM7314803.1"
                     /translation="MGERMELIEKAPAKINLGLDVLYRRQDGYHELEMVMSSVDLADH
                     LFFEEITEDKIIVETNKAFLPVDERNNVYQAALLLKNKYNIKTGVKIFVKKNIPVAAG
                     LGGGSTDCAAALRGINQLWNLGCSLEELAEIGLEVGTDVPYCVYGSTAFVGGKGEEIQ
                     VLPSMPQCWVVLVKPRMSVSTRTVFREVDTQAIQHQDIHGLAEAIREQDYQQMIKKMG
                     NSLEDITGKRHPVIHQIKERMIKYGADAALMSGSGPTVFALCQHYSRAQRVYNGLKGF
                     CEEVYLVRTLQ"
     gene            complement(50855..53428)
                     /gene="mprF"
                     /locus_tag="U2K71_000696"
     CDS             complement(50855..53428)
                     /gene="mprF"
                     /locus_tag="U2K71_000696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEM7314804.1"
                     /translation="MKNKRYLMLQWIKEHSLILKLIFFGSVLVFVANQVANIANGMSW
                     QDIWQRMGQQSRMTILLMILAGLLGVTPMLLYDWVTIRVLEKQGKPKMERKEFLLAAW
                     VTNTINNLAGFGGVVGASLRASFYGKQTNRKMVLATVSKVALFMLTGLSVWSFLTFID
                     VFFIQSESIFRSYWIWLFGGSFLAPVIFFFAYLKRKTLFSEFYPKGLLGLLLASFGQW
                     TGALAVFLIIGKLMRIDISMLSIYPMFMIATLIGMLTMVPGGMGTFDVLMILGMSQLS
                     VRQDVAVVWLLYYRLFYYVLPFMTGLLLFITHTGVKINRFLDNIPRLFFQKVAHIVLV
                     SAVYFAGIMMVLLSTITNLSAISRLFQVLLPFSFNFLDQTVNLLVGFLLLGLARALSV
                     KVKKAFLPTIALLSFGILNTITRTLSWQLILVYVLILLAVWLSRKEFYRTKFVYSWGA
                     IIFDASLFGFLFIVYAIAGYHSGRFLVGRITGNHFILFPSDDVWFSGLIGLGISLLAL
                     VALYQYLAEDTTKLGSEYEKDRFDRLLETYGGTATSHQLRLSGYSYFYYQEDDQDQVV
                     FGYQIKGNKCFVLGDPVGNPKMMRAATNQFMKQADCLGYQLAFYKISESYVVMLHELG
                     FHFTKVGEAGIVDLATEDLSRLSQQLEYQRLMKEGYHFTWYDQLPEELHQAVNEVSQK
                     WLQGAREKYFSVGRFDKSYVLSSGVGIVRKDQKVVGFITAQPMTKKQAGYDLLRVLPK
                     ESEELADYLLANLFIVYEKMGYSEANLGLAPLANVGETDFSFFQEKVMHIVYNYGNFF
                     YSFQSAYEKKLKYVDHWEGRYFAYRKGSNFGFATMQLLSLIGKEKGNVPSLAEEVLTE
                     L"
     gene            53569..54375
                     /gene="proC"
                     /locus_tag="U2K71_000697"
     CDS             53569..54375
                     /gene="proC"
                     /locus_tag="U2K71_000697"
                     /EC_number="1.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrroline-5-carboxylate reductase"
                     /protein_id="HEM7314805.1"
                     /translation="MKVGFIGTGNMAQAIIDGLLKKGTVSKEEVFVSSGHFENAQKFA
                     AETGVHACKSNQEVASQSDIVLLAVKPKIIEKVLEELREYSQKVLFISIASGKSLIDL
                     ETALEDPQASIVRVMPNVNVSVGAGVSAICKNEAATEEQLQQATMIFEAIGSVYPLAE
                     SDFSTFIGLAGSSPAFVYMLIDAMGRTGVLNGLSKQTATEIAAKAIMGSCQKFLSSDK
                     NPWELVDQVSSPGGTTVAGVVALEEAGFIPAIIKGINTTIEKDLEMLNKK"
     gene            complement(54454..56010)
                     /gene="rny"
                     /locus_tag="U2K71_000698"
     CDS             complement(54454..56010)
                     /gene="rny"
                     /locus_tag="U2K71_000698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HEM7314806.1"
                     /translation="MVFNILLAIIGLIVGLGLGFMVAKSRHEKEIAGAQSSAAGIIDS
                     AKKEAETLKKEALLEAKEENQKYRSEVESELRESKLELKSQENRLIQREQTLNRKDDS
                     LEKRENSLESKEEALSSKQQLIEEREKDVEKMIEDQQKELEKIAGLSKEDARDVIMKS
                     TEEELNHELTLMVKESEQRAKEEADRKAKNLLSLAIQRCAADQVSETTVSVVTLPNDE
                     MKGRIIGREGRNIRTLETLTGIDLIIDDTPEAVVLSGFDPIRREIARMTLEKLIQDGR
                     IHPARIEEMVEKSRKEMDERIREYGEEAAFEVGAHTLHPDLIKIIGRLRFRTSYGQNV
                     LKHSVEVAKLAGILAAELGEDIQLAKRAGLLHDIGKALDHEIEGSHVEIGAELAAKYK
                     ENPVVINAIASHHGDVEATSVISVLVAAADALSAARPGARSESLENYIRRLRSLENIS
                     NSFAGVESSYAVQAGREVRVMVKPEEISDLDAVRLVRDIRKRIEEELDYPGHIKVIVI
                     RETRATDYAK"
     gene            complement(56421..57470)
                     /gene="recA"
                     /locus_tag="U2K71_000699"
     CDS             complement(56421..57470)
                     /gene="recA"
                     /locus_tag="U2K71_000699"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HEM7314807.1"
                     /translation="MADDRKAALDAALKKIEKSYGKGSIMKLGEKIDQQISTIPSGSL
                     ALDVALGVGGYPRGRIIEVYGPESSGKTTVALHAIAEVQKNGGTAAFIDAEHALDPQY
                     AQKLGVNIDELLLSQPDTGEQGLEIADALVSSGAVDIVVVDSVAALVPRAEIDGEMGD
                     SHVGLQARLMSQALRKLSGSINKTKTIAIFINQIREKVGVMFGNPEITPGGRALKFYA
                     TIRLEVRRAEQLKQGTDIVGNRTKIKVVKNKVAPPFKIAEVDVMYGLGISQEGELLDM
                     AVEKDIVDKSGAWYSYKEDRIGQGRENAKIYMANHPEMMAEVSALVRAAYGIGEEVAV
                     PEDEKGQEELPLVEE"
     gene            complement(57579..58820)
                     /locus_tag="U2K71_000700"
     CDS             complement(57579..58820)
                     /locus_tag="U2K71_000700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence/damage-inducible protein A"
                     /protein_id="HEM7314808.1"
                     /translation="MKAEIIAVGTELLLGQVVNTNATFLSEQLADLGIEVYYQTVVGD
                     NQQRLEELIALAETRSELILLCGGLGPTEDDLTKEATAAHLGKSLIQNTEGYKKLLAY
                     FETTHRKMTKNNLQQSQIIEGGVPLPNRTGLALGTFYQTDTHAYILLPGPPNELKPMF
                     VEQVRPLLEERFPSEEKLISKVLRFYGIGESRLVTELKDLIETQINPTIAPYAKPNEV
                     TLRLTVKTNDVQAGNQALLALEEKIQERVGEYFYGYGDDNSLAKVVVELLKENKQTVT
                     AAESLTAGAFQAALGDIAGVSEVFPGGFVTYSLQTKAGFLEIDPELLAEYGTVSKECV
                     EQMAIQAREIARTDYSVAFTGVAGPDTLEGQSAGTVWIAISSKDGVVSEKYHFARDRA
                     AIRYNAVMQGFDLLRKQLLRK"
     gene            complement(59060..59638)
                     /gene="pgsA"
                     /locus_tag="U2K71_000701"
     CDS             complement(59060..59638)
                     /gene="pgsA"
                     /locus_tag="U2K71_000701"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HEM7314809.1"
                     /translation="MNLPNKLTVLRICMIPIFILVVGLPLDWGQFNVGDTTLAVTQLV
                     GAVIFALASITDWLDGKIARARGLVTNFGKFADPLADKMLVMTAFIMLVDQGKAPGWV
                     VAIIVCRELAVTGLRLLLVEHGEVMAAAWPGKVKTATQMVAIILLFINNIPFSLIGIP
                     VDQIMLYACLIFTIYSGVDYFIKNSGVFKGSM"
     gene            complement(59847..60770)
                     /locus_tag="U2K71_000702"
     CDS             complement(59847..60770)
                     /locus_tag="U2K71_000702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7314810.1"
                     /translation="MLRRIKQTGEDERVAFETIGEKLRQARLNKKISLDELQQITKIQ
                     KRYLEAIDNDDFDQLPGKFYVRAFIRQYAEAVGEDGDHLVDVFDGKSKLTGSVPERPQ
                     PETVSTSRKAMHQEESHPSRFWTSLPVILLGLVALAIVVVVGYMTWQDRQADPIIGDT
                     ASSLKVEGSVEESSSSEQSTQTKESSTVESTTKSSEEKKMAIATGEDSGSAIAVNITD
                     ATKPVTLEFTAVNRVWLGVLVDNAYVYQGTLAANETQSTVLPETATNATITIGAASNA
                     TIKANGESVPVNPGENNQSPKNVNLTLQYAE"
     gene            complement(61509..62795)
                     /locus_tag="U2K71_000703"
     CDS             complement(61509..62795)
                     /locus_tag="U2K71_000703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEM7314811.1"
                     /translation="MNKTEYEQINETLYHEVLPNGLTVYLLPKNDYHKTYGLFSTNYG
                     SIDNEFIPYGEKEKVKVPDGIAHFLEHKLFEKEDGDVFQLFGKQGASANAFTSFTKTS
                     YLFSTTDQVEKNLTTLIDFVQAPYFTEETVNKEKGIIGQEIQMYEDDPNWRMFFGILN
                     NLYPTHPLHIDIAGTVESIDKITAQDLYTCYRTFYQPSNMVLFVVGKMEPEKLMKLIR
                     ENQEAKNFPPKQEIVRYFPENTKEIIKQSALEAAITRDKFVLGIKGLDTLPQEGTELL
                     RYKTAINLLFQMILGNTSRNYLAMYNQGIIDDSFGFEFSLDREFHFADFSGDTDEPEK
                     AAEKVKEIILGFADDPEVSETNLDLLKKKMLGQYFQSLNSIEYIANQFTQSLFGDRTL
                     FDLPEIIDSIQMKDVLAAGEAFISEEAFSEFYMRPK"
     gene            complement(62788..64056)
                     /locus_tag="U2K71_000704"
     CDS             complement(62788..64056)
                     /locus_tag="U2K71_000704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HEM7314812.1"
                     /translation="MVYQLAEGVNLHVLPTKQYKTIRIFIRFTARLQQEVITKRSLLS
                     SMLETNSLNYSDQTKLSAQLAELYGASFGLSVRKKGNLHWLNAGISFVNGEYVNDPNL
                     FSQAVDFLKEVLFYPNIKNHQFDQLTFDLEKNNLRLYLESLKEDKQTFASYALQELYF
                     ENSPEQKIPGLGVVEDLDKITASSLASYYQEMMANDQIDIFVVGDVDPDKAAEAVGQL
                     PFESRETVYPDLFYTQPQVNIVKERQVREPIVQAKLNLAYQTNVYYDEPERFALMVFN
                     GLFGGFPHSKLFMNVREKESLAYYASSSVDTFRGFMSVQTGIDEKNRNQVLHLIHEQL
                     ESLRNGEITELELSQTKAMLRNQYLLSLDSPQAAIEASFLDSWLPETKLSDEEWLKRM
                     ESVTIKEIQQVAEQIELQAIFFLAGGNANE"
     gene            64308..64769
                     /gene="mscL"
                     /locus_tag="U2K71_000705"
     CDS             64308..64769
                     /gene="mscL"
                     /locus_tag="U2K71_000705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HEM7314813.1"
                     /translation="MIKEFKEFIMRGDVLDLAVGVVIGSAFTSIVNQVVQGLITPLIG
                     WFVAMITGTSDLEGALSILDWSPTKGVTFAFGNVISAIITFLITGFVLFLVVKAANRA
                     KNLRSKEEQVEVEETPTAEDYLSDIRDLLAKQVAEDNTHPVEQTNLSDTSK"
     gene            complement(64828..65274)
                     /locus_tag="U2K71_000706"
     CDS             complement(64828..65274)
                     /locus_tag="U2K71_000706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7314814.1"
                     /translation="MEYQVKKFEELSTAEFYAIVKERIAVFVVEQQCPYQEVDDIDGE
                     AWHTFFQDTDGKILAYTRVYEEAESIHFGRVLVHQDNRKDGLGRKIVQETINMIQKNF
                     PEKPIVIGAQEYLQAFYESFGFKAISDVYLEDDIPHIDMKKEQVAS"
BASE COUNT        18128 a        14122 c        11688 g        21390 t
ORIGIN      
        1 tttttggctg ccgtagcaac ttttatatca tatcagagag acaagcgatt gtcaactctt
       61 ttttaaaagt ttttttcgat ttcgtttttc gaaatctttc ggcaactcat ttatcataac
      121 ttatcgtttg ttatttgtca agaacttttt tgaagttttt ttcgatagaa tcaagcttcg
      181 caagtggttc ttttttaacg actcagttat cttatcatgt cgttagtaac atgtcaataa
      241 cttttttgtt atgtttttga ataaatattt tcttctgtct tttggacaag taataatata
      301 ccaactaaat ttagcttggt caacgaattt tttgtatttt ttcaagtttt tttcaataaa
      361 cgattattca taacttttct ttctttatcg ttcgttttcg tcttctttaa tgtctagaat
      421 agtcggcatt agcttttgaa ctttcgttat tatttattcc tttctacaca gcaaaagctg
      481 cctaaggaat tccttagaca gcttttattc gattatctca ttgttgggaa tagcaaaaca
      541 tcccgaattg attgtgcatc agttagcaac attactaatc tgtcgatccc gatgcctaga
      601 ccaccagttg gcggcatacc gtattctaag gcttccaaga agtcttcgtc tacaccgtga
      661 gcttcgtcat ttcctagttc acgttctttt tcttgcgctt cgaatctttc tctttgatca
      721 attggatcgt tcagttctgt aaaggcatta gcaaattcgc gccccactat gaatagttcg
      781 aatcgatctg tgaatcgagg atcttctgga tttttcttcg ctaaaggcga aacttcgact
      841 ggatgtccgt atacaaacgt cggttgtgtc aatgtttctt caacaaatgt ttcaaagaat
      901 tcattgataa catgtccgac acccatagct tcggttactt ctacattgtg tttttcggct
      961 aaagctaatg cttcttccaa tgtcatttct tgccagaagt ctacaccagt ttgttctttg
     1021 attgcatcta ccatatggat gcgtttaaag tcactttcta agtcgacagc ttgtccatca
     1081 tatgtgattt ttgctgttcc tagtactttt tcagctgcgt tacggataat accttctgtc
     1141 agattcatta catcattgaa atcagtatat gctgtgtatg cttctaacat cgtaaattct
     1201 ggattatgtg tagtatcaat tccttcatta cggaaaacgc ggccgatttc ataaactttt
     1261 tccattccgc caacaattag tcgttttaaa tgaagttcta gcgcaatacg caaatataaa
     1321 tcaatgtcca gtgcattatg atgcgtgata aatgggcgtg cggcagctcc acctgcctca
     1381 ttgtgcaata ctggtgtttc tacttcaata tagccattgc catctaaata gcgacggatt
     1441 tcgctgataa tttggcttcg tttcataaat cggtcaaagc tttcgcgatt actgattaaa
     1501 tccaagtagc gttgtctgta gcgttgttca atattcgtta agccatggta tttatctggc
     1561 agtggacgaa gtgcttttga caaaatcgtg atttctgaag ctttgataga aacttcgcct
     1621 gtgtctgttt tcatgatttg tccagttaca ccaaagaaat ctccaaggtc tgcatgtttg
     1681 aatacttcat aggcctcatc acctacttgg tcttttctta cataaatttg aatttggcct
     1741 tcacgatctt gtagatgcgc aaatccagct ttcccttttc ctcggcgagt catcatacga
     1801 ccagctacac tagcggtcaa tcccatttct gctagttctt cttttgttct tggatcaaat
     1861 agttcatgca attcttctga gttgtgcgtg cgttcaaatc gttttccaaa tggatcgatt
     1921 ccatcttcac gcaattgttc cattttttga cgacgtacaa gcatttgatc atttaaatct
     1981 tctacttgat gttgttgttc ctgtgtcaca aaagttcctc cattctcatg ttaatctctt
     2041 tcctataatg ccaatgaaac acaaaaaaag caagccgaac ttggaatttc ggcttgaaaa
     2101 ttcacgaaaa acttagaaat cacaacgttt gtttcaatag tactttttcc gctttttcta
     2161 caaattgatc cagcaggtca ttgatttctt gctgtttttc tgtctgattg attgctactt
     2221 tcactttcgc tgctcgagga atccctttta aataataagc cgcatgtgtt ctgaattctc
     2281 tacaagctac tttttctcct ttgagatctg ctaagcgttg taaatgaagt ttagctgtag
     2341 caattttttc ggctggtgtt ggttcaggaa tcagttcccc tgtttcgaga taatgctgtg
     2401 tgcggtgaat catccatgga ttgcccagcg ctgcacgacc aatcatcaca ccgtctgcac
     2461 cgacatagtc aagcatccat tttgcatctt ctggcgtttt gacatccccg ttccccataa
     2521 atggaatcgt caaatgtttt ttcacttctt tcaatacttc ccagtccgct tttccttcat
     2581 acatctgaac ccgtgtacgt ccatgcatag cgatggcact tgctccagct gattgtgctg
     2641 ctaatgcatt ttctacagca aagacgtgct cttcatccca accaatacgc atcttgattg
     2701 ttactgggat atttactgct gaagaaacag cttggaccat ttcgtatact ttctcaggat
     2761 ctaaaagcca ttttgctcct gcttctgctt tgattacttt atttactgga cagcccatat
     2821 tgatatctat tatatcggca gctgtgtttt cttcgacaaa ttgagcagct tcaactagac
     2881 tatctttatt tccgccaaat atctgtaaac ttaatggatg ttctgtttct tcaatgtgga
     2941 gcatctctaa agtttttttg tttctgaaat gaataccttg gtcactgatc atttcacata
     3001 cgactaagcc cgcaccaaat tctttgacag tgactcgaaa tgctgaattt gttattccag
     3061 ccatcggtgc aacaacgaca cgattaggga tttccacatt gccaattttc catgtgtgtt
     3121 ccacttaaat ccctcctaag gcttctttct tcaatccatc taattcttct tcgctatact
     3181 tgtatttgtt attacagaaa gaacaaaccg cttctgcacc gtgatcttcg tctatcattg
     3241 cttggatttc ttctgcacct aacgcgataa ttgcttctgc aaatttttct tttgaacagt
     3301 cacatttgaa ttgaacaggc attttttcca aaatgttcaa ttcttctgta ccaagcaatc
     3361 ctttcaatat ttcttctggt gtctgtcctt catctaagag ggtagagatt cttggtgtcg
     3421 tttttaattg ttcttcgatc ttgctgattg tttcttcatc ggcacctggc atgatttgga
     3481 tcatgaatcc tcccgccgtc ttgatgctgt cgtcagtatt gaccaataca ctcagtccaa
     3541 cagctgaagg gatttgttct gagatagcaa gatagtaagt aaaatcttca gcgatttctc
     3601 cagaaacaat cggcgtttgc ccagaaaaag gttcttttaa tcctaagtct tttgtgacag
     3661 taaacattcc ttgtgttcca actgctcccc gcacatcgat tttaccttca ctatttagag
     3721 gaagactgag atgcggattt ttgatgtaac ctttcacatc tccctttcca tttgcatcta
     3781 ctacaattcc tgttgctgga ccatcgcctt ggattcgaac agttagtttg tcttctcctt
     3841 tgagcgtcgc accaagtagc agactaccga ccaatgtccg tcccaaagca gctgaggagg
     3901 tgtgccaagt atcatgacgt ctttgtgcct ccgctacggt atttgttgct tgaacggcgt
     3961 atgctcggac gaatccgtcg tgagcaagtg ccttaaccaa ataatctttc atttgttatg
     4021 tactcctttt tccgttttta aactcatagt gaatcatact agcaactact gcttttttca
     4081 actttggaat gcttctttta aaaataattc agaatcatct agaaaacagg acatcaccct
     4141 atttacaact taaataaaga aatctcccga aaaccggttt ttgttccggt tttcgggagt
     4201 catatcagat caattgacct atattttttt atttgtattg atcgtctgaa tgattatctt
     4261 ctgattgttg agaatctgaa tcatgttctg ctttattttc ctcattcagt tcatgttttg
     4321 cttcttcaaa atcacgtttt tcttccgctt gtttttcagc gtctttttct tccaaagcac
     4381 gttttgcttc ttcaaaagtt ttcgcttctt tttcgcttgg ataatcattt gactcagtac
     4441 cttcaggcat cacaccgtgt tcaaacaatg acttgattgc tttggcatcc aatgtttcga
     4501 attccaaaag tttttcagca atcagtttat gctgtgcacg atgagcttcg atgatttctc
     4561 gagctttatc atgagcttcc atcaagattt ttcgtacttc ttgatcaatc tcgaaagcga
     4621 cttgttccga gtaagctttt gtttgaccat agtcgcggcc aacaaacact tgatggtttc
     4681 cttcatattg aacaggacct aaacgatcac tcattccata ttctgttacc atgcttcgtg
     4741 ctagagctgt cgcttgctca aagtcattgg aagcaccagt tgattggaca ttgaagatga
     4801 tttcttcagc tgtacgtcca ccaagtaagc ctacgatttg ttcaaacata tcttctttcg
     4861 tcatcaagaa ctgatcttct tttggtaaag cgatcatata tccgccggcg cgtccgcgag
     4921 ggatgatcgt tactttgtgg acgacccgag cgcgactcaa tactaatccg acaatcgtat
     4981 gaccagcttc gtgataagca accatttcgc gttctttttt gctgattacg cgatcttttt
     5041 tagctggacc tgcaatcacg cgatcttccg cttcatcgat atcagaagca tcgattttct
     5101 ttttatttct tctagcagct actaacgctg cttcattcaa gacattttct aaatctgctc
     5161 cggcaaaacc tggtgtctgt tgagccacaa ctttcaagtc aacgtcatct gccatcggtt
     5221 tattccgtgc atggactcgc aagattgctt cacgaccttt tacatctgga cgtccaacta
     5281 aaatctggcg gtcaaaacgg cctggacgta gtaacgctgg gtctagtaca tcagaacggt
     5341 tggttgcagc aatgacgatt actccttcat ttccgtcgaa gccatccatt tctacaagca
     5401 attggttcaa tgtttgttcg cgttcatcat gtccgccgcc cataccggca ccacgttgac
     5461 gacctaccgc atcaatttca tcaatgaaaa tgatagctgg tgcgtttttc ttcgctgttt
     5521 caaacaaatc acgaacacga cttgctccaa caccgacaaa catttctacg aaatcagaac
     5581 ccgagatcga gtaaaacggt actccagctt ctccggcaac agcttttgct aataaggttt
     5641 tacctgttcc tggaggtcct tctaaaagta cacctgccgg gatacgggca cctaattcaa
     5701 caaatcggcg aggatctttc agaaattcaa caacttcaac tagttcttgt ttttcttctt
     5761 ccgcacccgc tacatcagaa aaacgtacgc gattagcttt tttgtctgct tctttggctt
     5821 ttgatttacc aaagttcatg acacggccgc caccgccacc gccgccgcct tgttgtccca
     5881 tcatcatgta aaagaaaaag atgatgatta cgattggcaa gaagctaagc aatagggaaa
     5941 cccatatacc gctgcttgat tcttctttta ctgttgtagc aactttgttt tcttttgcca
     6001 gttctgttac ttggctcaat gttgtatcat ttggcaaaat gattgttgtg aaatgggtag
     6061 tagtcgtatc tgttgaacct aagatagcta acccaccgct gttagcgacg gtctgctgtt
     6121 ctttgtattc accagtgatc ttatatacac catttgcagg ctgcacttca aattctttga
     6181 tcttgcctgc ttgtagttgt tctgtgaatt ttgagtactc gatatctgac gatcgctggc
     6241 cgttattacc aaaaatgaaa taaacgacca ttaccatcgc taagattacg aggacataat
     6301 acaaggcatt tttcatcatg ccgttgttct ttttgttcat agttgccctc cctaagcgtc
     6361 aatttaattt aacagttttt tatcaatcct gaccattcta ttgtaccata atttaattga
     6421 gatacagcat taatttgatt gataaacact aggtttcaat acaccgatgt acggaaggtt
     6481 acgatatgct tctgcataat ccaagccata accgaccaca aattcatttg gtacgttgaa
     6541 gccaacataa tctgcttcga tatttactac tcttccttca ggtttgtcaa gtagtgtaac
     6601 aattttgact gattttgctt tgcggtattt gaaaagatcc actaaataag caagtgttct
     6661 tccgctgtca atgatatctt caacgatcaa cagatcgcga ccttctacat tcgtatctaa
     6721 gtctttgacg atttttactt cccctgacga gactgttgca ttcccgtagc ttgaaacatc
     6781 catgaaatcc aattccaaat acgtatcgat agaacgaacg atatctgcca tgaatggtac
     6841 cgctcctttt aagacaccta ctactagggg attggtgttt tggtaaattt ctgttaactc
     6901 tttgcctagt tctttgcaac gaacttgtat ctcttcttgt gagatcaaaa ttctctcaat
     6961 atccttttcc aacatcaggt tctccttcca attgcttctt tttgatattt gtaaagaagt
     7021 atgtagtgta ttttatcagt ttcttcacga atactcaaat atgaaaaggc aaaaggtatc
     7081 aagctatata tatttctttt ggcatctgta atgatccaag catgctgcct ttgtgaatta
     7141 ggtactttct gatcgataaa atatctagat actttttttg tgagattctc tgttaattta
     7201 atgcggtctc ctgcttttct tttttcgaca tacagttgca catcagcagg aatccaaatg
     7261 tcatgagaaa atagagacca tgaatctgtt gctttgatgt cccctgcatt ttcaggtttg
     7321 accagaccaa tccattctgt ttcattcaaa tatattcctt gttctggaat cagcaacaag
     7381 cgctggtctt ctgtctggct agttgcttgt ttttcttttt tagagagtac aacaaaatga
     7441 tactctcttt tcagtatcca attattgcca atactgagtt gccattggct ttttttctgt
     7501 tgccactggt ctaaaatcga ctccatttgt tgttctttca aaataattcc tgtttttgag
     7561 aatgctttgt cgaaaaagga gtaaaaaaaa tagtatcgtt cagcatctgt catttcttca
     7621 atcatttctg cagaaaatcg aaattgttcc tcccactgtt cgacttcctt ttcgatcaac
     7681 tgtcccatat atttttgaat gacttggtct gcccattgga tctgttgcga aaattgctgg
     7741 aaatgtctca acgcctgtgt attttcttgt ttcaattgag gaactaccaa atgacgaagc
     7801 cgatttctct ggatatctaa caattggttt gtctgatcct caaagtaaac aagctgcgat
     7861 tccgctgcat aagtatatag gtcttctttg ctaaaactca gcaacggacg aatcagtctg
     7921 cccgtagcaa atggttgtac ttctttgatt ccagaaaaag tatttaactg tcccccacga
     7981 acgattttca ttaacaccgt ctcgatttga tcatctccgt gatgagcagt catcaacgtg
     8041 tcatattgtc cttcttccat cacgcgagtg aattcttgat aacgaaagtt acgagcttct
     8101 gtttctatgg cggatttggc tggatcttcc caaatactta catataaagg gagattcttc
     8161 tcttcacaat atgagcgaag ataggcagct tcttctgctg attcttctct taattggtga
     8221 ttgatgtgga cgatacctaa agaaaaaccg atttggcgtt gcacttgtaa aagtaaatgg
     8281 agcaaaacca tcgaatcaac accacctgat accgctgcta aaattctcga tccttgttgc
     8341 cagaatcctt gttcattccc gtgccgaata aatgactgct gtaatagcgg tgcttttttc
     8401 ccgccttttt caacgttcat cctttccaca ccctttcttc ctaaaaaaaa gagagcggga
     8461 aaacgtctaa aacgacaatt aacctgccct ctgttgatac tatgacccaa atagtcgcac
     8521 tatcattatt tgcggctttt tagctgcgac gtccgccacg gccaccgcgc tttccttctg
     8581 tgtttcgctt caatgaagat aagcggtcat cgctatcttt caaaaatgaa ctcatcaatg
     8641 aatcgaagtc ttctttttga gctgaaggtt gactgcgaga aaaagatttt ttctgtgctg
     8701 gacgatttcg gttgtcacgg ggatcttttt gttgatattt tttaggacgg ctttcttctc
     8761 cagattcagc aggttgttcc tgcgctttcc ggatagatag tccgattttt ccatcatcat
     8821 ttactgaaat aactttaact gttacttcat ctcctacact taaaacatca tggatgtctt
     8881 ttacatagcc atttgacact tcgctaatat gaactagacc cgtttttcca gcacctagat
     8941 caataaacgc accaaaattt gtgattcctg atacttttcc ttgcagtttt gctcctacct
     9001 cgattgacat aaaaaaaatg ttcctcctaa ttatttccct tttttatatt cctgcttatt
     9061 ttgcagttgt agaactagtt gattgatctg tttcatttgt cgagctgtct gtatttgatt
     9121 ctaatatagg ataaactttt tcaccgtctt ttgagtaaaa gaatttgctt ctggcaagtt
     9181 tagctacata ctcatcatct ttcagcaatg aaacatcttg gtgcagctga tcaacgtctg
     9241 cgttgacttc tttcatttca gtatttgctt cgactctcaa ttcgttcaat tgatgcatcc
     9301 tttgcatgtc atggaaaatc tgcacaccta ttatcgcgaa aatgaccaaa gcaccaacaa
     9361 aaattgcagc cagtctgcgt cttcgaaaaa tcagttgacg ttgttgttgc tgaaattcag
     9421 catacttttt ctttgcatat tccgtatcta gcgcagcgat tttattgctt tttttcgtca
     9481 cttggattcg ctccaatctt tttgattgtc tttttcatta taccaatatc gtgaaagctt
     9541 gtctaatcta tttctctagt gtttctgtgc gattttcaga aataatttcg tacatttttt
     9601 gtgcatcttc ttttttggtt gattcatgta attccgtaat tttcacttcc aacgttttgt
     9661 tgccaaactg gactttcaaa atatcgccta ctttgacatc actggaagat ttcgccaatt
     9721 taccgttgat ttgaattctt cctttatcag ctacttcttt tgctactgat cgtcttttga
     9781 tgattcggga aatttttaaa aatttatcta atcgcatatc ttattcctca ctttttaatt
     9841 tttttattta atcgcaggat tttcttacca aaaggcaaca ttagccattc tcgtactgta
     9901 aacagacgaa gacgaatcac tgcataaatg aacacagaag ccccgatccc aactcctatc
     9961 acgctgacaa aaaaagcagt ggaacgatga gttacaggac caaatactat attcaataca
    10021 ccataataca gcagcagtac tactgtcata caaagaaggc tgaacatcaa cttccgtcca
    10081 aaatgccgtt ctttccaaaa agcatttagc tcttttgttt caattcgaac aaaataccaa
    10141 agcgtcacac ctaatcctaa aagggtagac aggcttgcac ctgctgttcc taataaaccg
    10201 gtcagaaaag aagtagtcaa tccttttacc aatagtcccc atcctgcgcc ttttaaagaa
    10261 gggcgaaacg agttcttgct ttgagcaata ctttgataag cttggatgac tgcagtgaaa
    10321 gcaatggaaa aaacaaataa tacaagcgct gcatttcccg cataatcttt aaacaacgca
    10381 taattgatat aagggagcag caaggctaat cccaaagacg cagctaatgc taaagcggtc
    10441 gtcaatctaa gatagatttt ggctgtctgt aaaaactgac gatagatccg gttggtcaaa
    10501 tgacgagtca acgcaggcag gaatgtcgca cttaatgcag tggcaatcac taaacctaat
    10561 tgtactaaag gctgacctcg atcatagacc ccttttgcta tcttcgcacc gtattctgac
    10621 agtccgtaaa cttccaaagc attttttaca aagaaagagt cgatcagctg gaaaaagata
    10681 agcaatccac tataaatcga aaccagtccg ccttctacca gaaaacgccg gatcaagggc
    10741 tttgacggct gtttgaacaa tggaaaccgt cggaagctta acgctcctcc atgaatcttt
    10801 tgttcatagt agtgaaggat cccatatgcg cataaaccgc caaaaacagc tccactcatt
    10861 gctgccgtcc ctgtttggta caccgtccat ccaagctttc taaaagcaaa cgcactacag
    10921 acaatcacaa aaacacgaac gaactgctca actacttgag aaacagcagt tggttccatc
    10981 aagaatcttc cttggaaatt ccctcgataa aaagatagac caggcatcag caaaaaggta
    11041 aaggaaacta cttggatcag cggtttcaat tgtatgtctc ccatcatcca agcaatccag
    11101 ctgctaaatg caaagacaaa caaccacaaa aagacacctg tccaaaatac taagggataa
    11161 agattttgca aagcttgctt tcgctcgaaa ggattctttc tttctgcaac gtactttgat
    11221 atgaactgcg gaagaccgga taaagctaaa gtcattgcta tcccataaat tggataaact
    11281 tgctgataaa cataaaaacc ttcgtcgccg actaagtttt ggaggggaat tcggtaaagg
    11341 gcacttaata cttttgctat gaaagaagca atcgtcaata caaatgctcc ttgcatgacg
    11401 cgccgcattt ctttatgatc caaatcattg ctccttccta cgctgacaga tatttttgct
    11461 cacgcaacgc tttgacaaac ttttggattt cctgaagcca tactgcttct tccatttggt
    11521 tggggatcgt caagcgtatc accatctgtt ctttttcaac gcctagactg gcttttagtt
    11581 ttgtcgctgt cagtgcttcg aataactgtt ccacactata gcgttttgtt ccaactttac
    11641 ttagagtaaa gactatatgc gcctcctgtt tccttatcgt ttctaggagc gctcgatcgc
    11701 catccatttt gatctgccca attgtcaaga gatgtgctac ttcttctgga tattctccaa
    11761 aacggtcgag cagatcatct tctagttcat ctagcatttc ttgcgaatca agttcacgaa
    11821 ttcgtttata gatctcgatt ttctgccgtt gatctgccac gtatgtttca ggtagatacg
    11881 catcgacacc gaggtcgatc tcaacagtgg tcttctccac ttgactattc tttccttgtt
    11941 tacgagaaac agcttcgctt agcatttgag aatacatatc gaaaccgact gcatcaataa
    12001 atccatgctg ctgagcacct aagagattac ctgcaccgcg aatggaaaga tcccgcatcg
    12061 caatcttgaa tccagatccc agttctgtaa aatctttgat tgcctgaaga cgtttttcac
    12121 tgacttcgtt aaggatcttc tgctgctcat acataaagta agcatatgcg acacggctgc
    12181 ttcttcctac ccgccctcgc aattggtata acgtggataa tcccatataa tcagcgttct
    12241 caacgaacaa cgtatttgcg ttcgggatat ctactcctgt ttcaatgatg gttgtagtga
    12301 caagaacatc atattgacct tcaataaatt caaaaagcgt attttctaat tgcgcctctg
    12361 tcatttgccc atgagcataa ccgatccgtg cttccggaac gagttcttgg atttcttcta
    12421 ctttttgttc gatcgtttct acccgattat ataaatagaa tacttgtcca cctcgtccca
    12481 tttctcgatg gattgcttcg cgaatagccc caggattttt ttccataaca tacgtttgga
    12541 tcggataacg attcgcaggc ggtgtttcga ttactgaaag atcccgcacg cctaacatcg
    12601 acatatgcaa agttctcgga atcggtgttg cagtcagagt caaaacatcc acttgtgcac
    12661 gtaactgttt caatcgttcc ttatgtttta caccaaaacg ttgttcttcg tcaatgatca
    12721 gtagacccaa atcgctaaat tcaatatctt ttgacaaaat tcggtgggtt cctacaacaa
    12781 tatcaacttg acctgtacgc agttgttcga tggtttcttt ttgttgcttt cttgttctga
    12841 aacgacttaa tagcccgata tttacgggga aaccttcaaa tctttccagc atcgtttcat
    12901 agtgctgctg tgccaagatc gttgttggaa ctaaaaaggc tacttgtttg ctttccttta
    12961 tcgctttaaa ggccgctcga agcgctactt ctgttttccc ataaccaaca tcacctacga
    13021 gtagtcgatc cattggtttt tctttttcca tatcccgttt gatctccgct gcacttctca
    13081 actggtcatc agtttcagag taggggaagg cattttcgaa ttctttttgg taggcgtcat
    13141 caggtccaaa agcatatcct ttttctgact ctcttgcggc atataatttg attagatcgt
    13201 cagcgatatc ctcgattttt gaagagactt ttcgcttggt tttcgtccat tcacttccgc
    13261 ctagcttatt gatgcgaggc gctttggctt cagaggcgac atatttttgg atcagattca
    13321 gttgagtaac aggaataaac agtttgtcgt cgttttgata caaaatagtc atatagtctt
    13381 ggtgaacgcc atctacttct agcgtttcca tcccaatgta tttcccgata ccatgattgg
    13441 catggacaac gtaatctcct gcttttaact cattatagct tttcaatcgc tctgcgtttg
    13501 ttactgtttg acgtctcgtt tgttttttcg tcgttttttg gaaaatttct ttttctgtga
    13561 tagctactat tttttcttga ggaagttcaa aaccagcctg caaagctcct tcaactaatt
    13621 gtacctgtcc tctgatcaat gcattttttt ctgtttctac tactaatatg tcttcatctc
    13681 gaagcatttg ttctgctttg cgtattctgt cctttgtcgg aataaagatc acaacagtct
    13741 gtttttgctt gcgccaacgg tccatttctg ttttcagcaa tggcatttgt ccgaagaact
    13801 gttgcatcgt ccgatattga aaattatgga ttgcttggaa acgaagattt cccatccctt
    13861 tttggaagag tgagaaaaag gttgtggtga acttttcctt ctgcacgatt ttttgtatat
    13921 ctgctgaaaa tgtctgctca gaaaacaccc gcaattcgct gattttttga gtctgccatt
    13981 ctgccgcttc cctttcgatt tcccgatttg tttccatgat tcgctgataa tcatcaacga
    14041 ctagtaaact atttgaaggg aaataatcca agatcgtcac agattcttgg taaagaagat
    14101 ctgagtagaa ttttgcttct tctgtcggga cacctgcttg ccagcttgaa gtcagctgac
    14161 cgaagtaatc ttgcaaaaac tcgcggtctg cttcttctgt ggtcgcagca agccgttttt
    14221 ctagcatttt ttgcatttgc tctattccat gttctagatc ctctggtccg tagacttgtt
    14281 cactcgttgg ggaaacccag atttcttccg ttttttcgat tgagcgttgt gtttctgctt
    14341 caaaataccg catagaatct atctctacat caaatagttc tacacgaaca ggataggttg
    14401 tcgttaacgg gtaaatatcg ataatgcttc ctcgaatact gaattctccc ggttttgcga
    14461 ccatctcttc tcgtacatag cccatcaaag tcaattggcg aggaagattt tccacgtcta
    14521 tctcatcacc aatatgccaa ttcaattgcg cctgtagcca agttttctta ttcggcagtc
    14581 gtttgtgcaa agatgcgact ggcaacaaat agatacctgc ttttttttct tgaagtgcgt
    14641 taagcgttgc aactctctcg cctcgcgctt ctggagaaga aaaggccatt tcagcagaaa
    14701 gcacttcatc cactggaaat aaatggatct gatcttgcgg aatcacatgt tgcagatctt
    14761 ctgctaactg attcgcataa tacaaatttg gcatcagaat aatcatgttt ttatttgttt
    14821 tcttaaacgc tcgagcaaat agaagtgttt tcgctgagcc cgctaatcct gtgattagtt
    14881 gtctaggcat tttttcttct aaattgctga cccattcttt tacttgcgga tcttttccga
    14941 ctaattgaat aatatccatt ccttttcctc cttcctcaat tgaatcgatt catcgcatcc
    15001 acgaacgaat gcccttctat gagatattcg actgctttaa cagattgaca catgctttct
    15061 tctatcaaag gctgattttc tttgctgaaa ggactaagca catgctggac tactgttttt
    15121 ttcccttctg gcctgccgat tcccaccttg attcgatcaa agacattcgt attcaaatgg
    15181 gaaataatac ttttgatacc gttatgcccg ccggcacttc ccttttgacg gagccggatt
    15241 tttcccactg ccagatccaa atcatcgtag attacgacta attcctctgg ataaatccca
    15301 aaataagtca ttagtgggcc gactgcacga ccagattcat tcatgaatgt ttgtggtttg
    15361 actaaaagga tcttttctcc attatgaaaa aattcggcaa cttctgcttc gaacggattt
    15421 tttttgaaag tagcttgatg ctcgcgtgca attttatcca cgaccataaa gccgatattg
    15481 tgttttgtat attggtattt ggtacctggg tttcctaacc cgacgatcat tttcattttt
    15541 gttttccacc taattctaaa aagcagcaca aaaggctgga ctgctaatta gcggtccagt
    15601 ccatcgagct tttttctttt ttacacttgt caatgcatag tataacacaa aacaaggcgt
    15661 ttcggctaaa agagaatcgt taataaatga taaagaaatt aaaaatttct tcaaagttta
    15721 atgaaataat tgatatataa aaatagtgaa ataacgcaca tgtaagcttt tacatataat
    15781 acaaccttgt tatctgttat attttatagc caaatagcgt gacatggggc ttgagaaatg
    15841 ttagcataag catgggaaaa aacaaatcaa tggaaggatt gatactaaat gactgcagct
    15901 gcagaaaaaa aagatcatca aaaagttatt cttgttggag acggcgccgt aggttctagc
    15961 tatgcctttg ctctagttac tcaaaatatc gctcaagaag taggtattgt ggatatcaat
    16021 actgctaaaa ctgaaggaga cgcaattgac ttgtctcacg ctttggcatt tacttcacct
    16081 aaaaaaatct attctgctac ttacgcagac gcacatgatg ctgacttagt tgttattaca
    16141 gctggcgcac cacaaaaacc aggtgaaact cgtttagact tagtaaacaa aaacctaaaa
    16201 atcaaccgcg atgttgtcac acaaatcgtt gaatcaggat ttaacggtat ctttttagtt
    16261 gccgctaacc cagtagatat tttgacttac tcaacttgga aattctcagg tttccctaaa
    16321 gaacgcgtaa tcggttcagg tacctctctt gactcagctc gtttccgtca agcacttgcg
    16381 gaattagttg acgttgatgc acgtaacgtc catgcttaca tcttaggtga acacggagat
    16441 tcagaattcc cagtttggtc acatgcaaat gttgccggct tgcaaatcta tgaatgggta
    16501 aaaaacaacc cagaaatcga tgaagaagct atggttaacc tattctttgg cgtacgtgac
    16561 gctgcttaca caattatcga gaaaaaaggc gctactttct atggtatcgc agtagcatta
    16621 gcacgtatca ctcgtgccat cttagatgat gaaaatgctg ttcttccttt atcagtattc
    16681 atgaatggcg aatatggatt gaacgacatc tacattggtg cacctgctgt tatcaaccgt
    16741 caaggtatcc aaaaagtaat cgaaattcct ttaagtgaca gcgagcaaga ccgtatggct
    16801 gcttctgcta agcaattaaa agaaattcta gatgaagctt ttgctaaact agacgctgaa
    16861 taatctttga acatgacgga aaacatctat tcggtgtttt ccgttttttt attttatcac
    16921 ccttatggtt tctaaatttt ttcataaagt agagataacc ttcgtctttt cctctaaatt
    16981 atgctatcat gagaccgttc caccctaaac ttttacttaa ggagttgtcg atatgacaag
    17041 atcttcgcat cgaaaaaaat cgaaacatgt tgctttgctt tcagttcttg gtgttctctt
    17101 agtgctgatc agcttttata cctatcgcag tacatactac acaaagcatt ttttgccgaa
    17161 tacagaaata aatggaataa atgtcagcaa tctaacagtc gaaaaagcaa atgaaaaatt
    17221 aaaagaagct tattctgata aaaaactggc tattaaagaa gatggaaaag tctggcagga
    17281 agtaccaaaa tccgagctcg gctacaaaga tgatttcact tcggagcttt ctccgatcct
    17341 aaatgaacaa aacggatgga cttggggaat gacttatgtc tcagcggcag aaaaacaaga
    17401 aatagatcct gtcagtcaaa accaccaaaa actagacact accgttcaga cattgacaac
    17461 taagctgact gatttgaaca aagaccggac acctactact gatgcaacga tcgaaaaagc
    17521 aggtgactct ttcgttatca agcctgaagt caatggaaat acgatcgacg tcgatgctgc
    17581 ggtcaaacaa ttaaaaagtg cggtaaactc tggtaaagat acaattgaat tgactgaatt
    17641 caaagaaaaa ccaaaagtaa catctgaaga cagctcactg aaagaacaac tcgcatccat
    17701 gaatgctatc gcaaatgtaa aagctactta cagtataaac ggtgagactt tccagatccc
    17761 atcaagtgat attatgagtt ggctgaccta caatgatgga aaagtcgatt tagatacaga
    17821 acaagtacgt caatatgtaa cagatttagg cacaaagtat aacacaagta cgaatgacac
    17881 aaaatttaaa agtacaaaaa gaggagaagt tacggtccca gttggcactt acagttggac
    17941 gatccaaaca gacagtgaga cagaagcatt gaaaaaagcg attctggcag gccaagattt
    18001 tactcgttca cccattgtgc aaggtggcac aaccgctgat catccattga tcgaagatac
    18061 gtatatcgaa gttgatctgg agaaccagca tatgtggtat tacaaagacg gaaaagtggc
    18121 ccttgaaaca gatatcgttt ccggaaagcc aactacacct acacctgctg gagtcttcta
    18181 tgtttggaat aaagaagaag atgcaacttt aaaaggaaca aatgacgatg gaacacctta
    18241 cgagagtccg gtaaactact ggatgccgat tgactggaca ggtgttggta tccacgattc
    18301 agactggcaa cctgaatacg gtggagactt atggaaaacc cgcggttcac acggctgcat
    18361 caacacccca ccaagtgtaa tgaaggaatt attcgggatg gtcgaaaaag gaacgcctgt
    18421 attggtcttc taacaaaaat aatatataaa aagaaagcac ccgtacgatt ggtacaggtg
    18481 ctttcttttt attatctaac aatgtgctac ttacatttca gcagcagtaa agacttctga
    18541 aacatcatca tcgtcttcta gcttatctac tagacgttcc aattgctctt tttgttcctc
    18601 atttaaatca atcgtcgttt gtggaatcat cgtcaattct gcttgcgcca aagtgaactc
    18661 tttttccaac tcatctcgta cagcagcaaa atcttctggt gcagtataaa tctcgaatac
    18721 ttcaggagaa gtttgcagat cttctgcccc cgcctctaat acatcctcaa acatcgtctc
    18781 ttcatcaaca gtcaacccat cgcgttcaat agcgatatat cctttgcggt caaacaagta
    18841 actgactgaa cccgtttctc ccagtgatcc accgtttcga gtaaaagcta ctcggacatt
    18901 tgtcgctgtc cgattacggt tatctgtcaa tgcatggact aagattccta ctccacccgg
    18961 accatatcct tcatacgtga tttcatcgta attcgtttca tctacggcac tcgtagcctt
    19021 tttaatcgcc cgttccacat tgtcgttagg catatttgca gcttttgctt tgtccacaac
    19081 aagccgcaaa gctggattca tagatggatc aggaccgcct gctttagctg ccatataaat
    19141 ttcacgagaa agtttctgaa agactttccc tcgttttgca tcttgggcat tttttcttcc
    19201 ttggatatta tgccatttac tatgtcctga catgattttt cctcccttgt tcttttgaga
    19261 atacacttct attctagtac tatttcacaa aaaaacaaag ccacagccaa tctataaacg
    19321 cttcttgaat gccgcaaaaa gcacacataa tatcacagca gtcagagcac cgatcgaatc
    19381 caacatgaca tcttggaaca taggtgttcg tccaccggtc atcatctgat ggaattcgtc
    19441 caaacctgcg taccctgttg ctgaaagcca agcaaggatc gctgtcagcc accaaatttt
    19501 tagcttgggg aaaacaccta aaaacaagct gcctcccaaa ataaaatagg taccgaaatg
    19561 tgcgcccttc cttaaaaaga actctaaaaa tttggcgtag cccagatggt tgatactcac
    19621 catacttcca ccataattaa aacgtatacc cgctaatgtg tctttaaatg gctgattagg
    19681 cagccagctt tctaatcgcg aaacttgtga ttgctgtccg taagtttgag aagaactgta
    19741 aaataagatt ataacgacta tcagtgcgat tactaaataa atatttccgt ttttcatttg
    19801 tttttttgaa gaattcataa gtcacctctg tattttatct tagacttttt ttgaagaaac
    19861 aacaatcatt ttcatagaga cttcttccat aaaaaagtgc tatacttaat tttgtattac
    19921 aatatgaagg gagttttttc atgagttatt cattaaattg gaatctagat tctatttttt
    19981 ccggaggcag tcattctgat gctttgaacc aacggatgaa gcaattggaa gaccaaatga
    20041 acgagtatta tcaccgcgta acaaaatggt cacctagttc tgacaatgca gagcagttaa
    20101 acgctattct tcaattgcaa gaaacgatta cgaatggttt tacccaatgc agcagctata
    20161 ttactgcatt gctttctgcc aatgtcaatg attcagatgc aaaggtctta tcaggcaagc
    20221 tttacgcaat gctgcctcgt ctgcaatcag ccgaaactgt attgtcaaag aaatttgcag
    20281 aaatatctga caatgattgg aatcagatgc tttctcaatc ttcgtttgaa acaattgctt
    20341 ttcgtttaaa cgagattcgt cgtgatggct cacaattatt atcagaagct gaagaaaaca
    20401 ttataaatac gctttcttta gatggtctaa atgcttggag cagtcattat gatacgattg
    20461 tagccagcat ttcgattccc ttcgaacaag atggacaagt agtcgaatta tctgctggac
    20521 aagccttcaa taagatgatg ggcgatccag atccaaaagt cagagaaaca cttttcgcag
    20581 catgggaaga agcctggaaa gaaaaggcac ccctcttcac ggatactttg aatcatttag
    20641 atggttttag actttctgac tatgaacttc atggtgtcac agattttcta caaaaaccat
    20701 tagagtacaa tcggttgaag aaagaaacat tgtctgtcat gtgggataca atccagaaaa
    20761 acaaacaacc ccttgttaat tacttgacta gaaaagctaa tcttttcggt aaagaaaaaa
    20821 tggaatggca agatcaagat gcacctatta ttttaggcga tttgaaagaa aaaaccttta
    20881 gttttgacga agcagcagca tttattatcg aaaactttaa caaattcagt ccaaaaatgg
    20941 ctaaatttgc ccaatctgct tttgaaaaga gctggatcga agcagaagat cgtcctggga
    21001 aacgtcctgg tggttattgt actgagttac cagaaacaaa agaatcaagg atctttatga
    21061 cctacagtaa ttctgttaat gaggttgcga cacttgctca tgaactagga catgctttcc
    21121 atagcagcac gatgtgggat ttaccttctt taaatcgcga atatgccatg aatgtcgcag
    21181 aaacagcaag tacattcgca gaactgatcg tagccgatgc tacattaaaa gtcgcaaaaa
    21241 caaaagaaga aaaaatcaat ttgctagata caaaactgca aaatgcttta gctatgttca
    21301 tgaatatcca ttcccgattc atttttgaaa atcgattcta tgaagcacgg caagaaggat
    21361 tagtctcaga agataagatc acagagatga tggttgaggc gcaaaaagaa ggctatcaag
    21421 gagctttagc tacttaccat ccatatttct gggctgcaaa attgcatttc tttattgatg
    21481 acgtaccgtt ttataacttc ccatacacat ttggctacct ctttagtttg ggcatctatg
    21541 cttacgccaa ccaaaaggga gcaaattttg aagaagaata tatccattta ctacgcgata
    21601 ctgcctcaat gacgacggaa gatcttgcga agaaacattt gaatgtcgat cttacccaac
    21661 cagatttttg gcaagcaggt atcgacatgg tgactgaaga tatccaaacg ttcatggaat
    21721 tgacagaaga atttatctga tttctatttt atcacaataa aaaaagagtt agtccagaat
    21781 gggctaactc tttttgatta atcattctta catgctgaca gcaaagtcag gggcaaacca
    21841 ttgaactgaa ccaactttta cgctttcgcc tggttgagga gcatggatat attgtcctcc
    21901 gcctaaagca atcgctacgt ggtaagttcc gccttgtgac ccccagaata acaagtctcc
    21961 agcttttgct tgagaaactg aaatttttgt tccagctgat tcttgaggaa ctgtccaacc
    22021 accgatgtca cgaccagtta cttgcatgta aacatagcgt gtgaaccctg agcagtcaaa
    22081 gccacttgga tctttaccgc cccaaacata tggagtacca atatatttgt aagcttctgc
    22141 tacgattgct gcaccattca cacttccaga aggagctgga gcaggtgtta caggttttgg
    22201 ttgttcaggt gttggtgttg gcgttggagc cggagtacta cttcctgtgc cgtttcctgt
    22261 atcaacactt tgatctgttg atggtgttgt aggcgcaggt gttgttgatt cttctgttgt
    22321 tgaagattca ggcactgttg ttgattcttc tgttgctgaa gattcaggca ctgttgttga
    22381 ttcttctgtt gctgaagatt caggcgctgt tgttgattct tctgttgctg aagattcagg
    22441 cgctgttgat tcttctgttg aagattcagg tgttgttgat tcttctgttg ctgaagattg
    22501 tgtagctgat gatgattctt cagtcattga agattgtgta gctgatgatt cttctgttgc
    22561 tgagctttgt gcagtagatg aagcttgagt agcttgtgct tcagcctctg cttgttcgcg
    22621 tgcttctttt tcagcttttt cttgtgcagc ttgttgacga gcttgttctg ctagacgttg
    22681 ttgttcacga atacgagctt gctctgcttc agcttctgct ttttgacggt tcaagtcagc
    22741 tttcttgtct tcagcagttg cttgttcagc agctaatgaa gttttcagta cgttcaaatc
    22801 agcttgttta gataaaagat cgcctttttg agattccaaa gcagcttggt tttcagctaa
    22861 ttcttttagt ttagcatcgt tttcagcttt tttgtcttct acagcttttt tatcttgttt
    22921 ttgttgttcc atcaagtcgt tgtttgcttt taccatcgtt gtcattgctt gaacgcgtcc
    22981 aatagcatct gctaatgaat cagcattcaa tacagcatcg atgtagtttg agcttgtgtt
    23041 gcttacttgt gcttcgcgtg cttgtttttg gattgtatct tcacgttttt caatacgttc
    23101 ttgtaaatct gcaatgtctt ttactaattg tgcagattct tgacgcaatg tatcttgttt
    23161 tgctaatagg tcttgtgctt gtgtgttgat ttcagaaact tggctttcta aggcatcgat
    23221 ttgtgattgt gcatctgctt gttgattttt caagtctgca atcttttgat cttgctgttg
    23281 tatctgagaa tcaaaatcgt ccgccgcggc agcgatcggt gatgctacgg cagtcaaggt
    23341 cattgaacat accattactg ctgatattaa actctttttc actcttcatt cctccgactg
    23401 gcttaattta ataaatatct aaatatattt aagtctttct taacgatata aaaattgtac
    23461 catagcagtg tgtcaacgat ataatagtta tgttacaaaa atgtttcaaa acaactttta
    23521 aaaatgacaa aactgttcgt ttcagaaaat gatcatgaaa aagccgcgtt aagtctagca
    23581 tttcttaaga taacaacgat tctttttcgc tttcgaccta aaaataaaaa aagaaattat
    23641 tcatcactga ataatttctt aaagggaaaa atgaataata caaatataat catgttcaac
    23701 agcagtgtag gtcctaaaaa ccttgtaata aaatacgtgt tatttactac tgctaattta
    23761 aaaatcaact ggaccaccat cgtaaacaat tcataacccg tgacaaagat gatcatactg
    23821 aaaaattcag taaagatgtt gacatggata acgtctttca ttacatacat caaccataca
    23881 attaaaggga gagctactgc atagataccg ataacgccaa tatagtaagc gtcatagata
    23941 gccccgagaa caatcgtggt aatcagtaaa taacgttttt caaacgccat actgcaacat
    24001 aataatgcca atatcagaaa gtgggcattt gacatatagg tgcctttcga ccactcccct
    24061 agcattcttg ttaaatgacc atctatcaac atcaaaaaga acaaaacgat tggcagatag
    24121 tacttcattg tttctttctt caacatggac taatcctccg ctagccgttg gacaatcgtc
    24181 acaactgaca cgtcatacat ttctgcataa ggtttcacat aaacttcccg atccagccca
    24241 taactatctg gtttcgtttt aataaccgta ccgatcggca agttagctgg agaattccct
    24301 ccaagaccgg atgtctggac aacatcccct tctttgatat ccgtatcacc agttaattgg
    24361 gtcaccacta aggcatggag cttttcatca tagtttttaa gcaaaccaaa cgcttcgcca
    24421 ttagccgaag atacccgtac tggaaaatga ttggagcttt cattagagga tgacagtaat
    24481 tcgattttag acgaagccgt attgacctcg atcactcggc caatcaatcc tttttgcgac
    24541 atgactgcca tatttgcttc gataccgtct ttagaaccct tgtctacgat cagcatgtcc
    24601 tgccaactgt caggcgaacg agtaatgaca tttgccgtta ctttttcgta actagtcagc
    24661 gtctgattca actcaagttc ttttttcaac gcttcgattt ctctctcgta attcttgttt
    24721 tgctgtgaaa cgtcatcata gctatcgatt ttttctttta gccgctcatt ttcggcataa
    24781 gttgaaaaaa gattatggac agaatctact ccatttcgta cccaccgtac tggggcagag
    24841 attgttttat caaccattgc aacactgtca ttaacgatgg attggaagaa attgggcttt
    24901 ccttcattcg ctcgctgcgc agcggtcaca cttagtatcg tcaccacgat gataacaata
    24961 accaaggtaa taataatatt tttattagga ttgaacttct tcacaaagac acctcgaatt
    25021 aattagactc ttgtgttcaa ttttatcact aaatgccgtg aaacaaaagg taagttgctc
    25081 ctcttaagct tttttttagc aaataataga ttctgtcatt tttagcctga aagacaaatt
    25141 tttttatttg tttatggatc aatctttgct ttgaataact gcaatcatac catctgaaaa
    25201 agaaaattct gcggtatctg aaagaaattc gttacttgca taagatgtcg gatattctac
    25261 ttctttattt tccaatacat acttgctgcc ttttaatgta agattcgaga cgggagtcaa
    25321 gcagcaataa gctaaatact tcatatctgg tatctgtctt actatatgct ctccaggcaa
    25381 ataataagac aaataatttt gacaatctct gataataagc tgatgggcaa agggtttgaa
    25441 gcgaggttcc atacctaacc agagattggc taaaagatga tccaatcttc cgcccgtagc
    25501 tccaatcaaa gtgacttccg cttgcgggta acgttcaaat gtttttaaga tacctaactg
    25561 tgtatctgta tcgtcttttt ctgcagggga acgggtatac tctttggctt ttgcaaatac
    25621 ttccttctgc tcttcgttcg acaatgaatc aaaatcaccc actgccagat ctactgggta
    25681 gcctgcttga agcaggtaca gcgcaccacg gtctatcccg acataggcat cgtatttgga
    25741 cagcagagac tgcggccagt tttgcggatt acctccagca gctaataaaa ttttcattta
    25801 gtctaatgcc tcttttaatc gttcgatttg tccgactgga ttttcggctc catagatata
    25861 tgatcctgca acgaagacat ccgcccctgc atctttacag atttttgctg tttctggaac
    25921 gattccgcca tctacttcga ttgcataagt ccaattgttc tctctacgta gatcagcgag
    25981 ttccgtaatt ttttctactg tttcttcaat aaagctttgt ccgccgaacc caggattgac
    26041 agtcatgact agtacttgat cagccaaagg caaaacatgt ttgatcatgg agactggtgt
    26101 tcctggattg ataactactc cagctttaac tcctaagctt tttgtcattt gaagtgcccg
    26161 atgaatatga ggagttgctt cgacgtggac agtaatgata tctgctccag cttttgcaaa
    26221 tgctggaatg tagttttctg gatcaacgat catcagatgg acgtctaacg gtaatttagt
    26281 aacaggacgg attgcctgaa caatatttgg ccctaatgtg atatttggga caaatcggcc
    26341 atccattaca tctacgtgga tgtaatctgc gcctcctttt tccactaact caatgtctct
    26401 ttgtaaattg gcaaaatctg cactcaaaat ggatggtgct atcttcattc ttgtttcctc
    26461 ctattttcgt ttaccttctt tttttgcata cattggtttt cttttttcga tttcttgtaa
    26521 gaattgcaaa taattgtcgt aacgtgtctg tgcgatacca cctgtttcta ctctatgttt
    26581 tacttcgcag ccaggttctt tagcatgcat gcactctcta aatttgcaat ggacagaagc
    26641 ttctacaaat tcaggaaatt gtctcggcag ttctcctgct tcaattgtca aaaagtcaat
    26701 cgagctaaat cctggtgtat ctgctactaa cccgccatag ataggaatca gttccacatg
    26761 acgtgttgta tgccgtcctc ttccaagtgc atcagaaatc tctcctgttg ctaaattcag
    26821 ttccggtgat atttgattca gtagcgtcga ttttcctgct ccagactgac ccataaagac
    26881 cgatagccgt tctgggaagt aacggatcag ttctgtcgta tcacccgtat gattgggtac
    26941 aatcaccgga tagcctattt tagtataggt ttgcttgatc tcttcgattt tttctgggtc
    27001 gtctgacaag tcggtctttg tcaaaaaaat aatcggttcg atcgactcgt attccaaggt
    27061 aactagaaag cggtcaagta gattataaga aaatgcaggc tctacgatac ttgtgacaat
    27121 gattccaaga tcaacatttg tgactggtgg gcgaactaat tgattttttc tcggcaagat
    27181 ttccagaaga tagccgtctg tttgattttc actctcaaag atcacctgat ctccgacaag
    27241 cggcgtgatt ttccgattcc ggaaatttcc tcttcctctt gtttgatagg tctctccgtt
    27301 tgcgtataca tagtaaaaac cgctgattgc ttttctgatt tgtcctttga taagtgccac
    27361 ctccgttttt gtttatttta ccatacgaag aggaaagtag gtgttattcc atgcaaactt
    27421 ctcaaagtat atggcttgaa aattgatcaa aagagataga aacacaaaaa agagaatgca
    27481 acaaatcaat gtcgcattcc cttctgatat attttattca gcacttattt tgaactttct
    27541 gaggtgctag ctgatgaact tgtggagctg gaagattctt cttttgaaga gttgcttgtg
    27601 ctactggagc tacttgagct gctagaactg tctgaagagt ctttttcaga actagaagac
    27661 gctggatctg gtcctttaga gtaaattacg ttcacaacca ttcctttggt aattgtcgtt
    27721 cctgcccctg gtactgtacg aatcacttta tctttatcca ccgtgtttga atattcggaa
    27781 gtctcatgcc cgatatattc tgcaccgaca ctagctaaat aactttgtgc ttcgcttttt
    27841 gtatagccgg agatatcatt caatttaggc tctgttcctt ggcttacgac aaaattgatg
    27901 taagtttctt ctgctacaac ttcacccgag gctggatcct gactgatgat cgaacctgat
    27961 ggtacagtgt cgctatagtc ataagtagca ctaatattat tgatattgaa tccttgtgta
    28021 acaagccaat tacgaacagt ttcgtagttt tctcctacat gactactcaa agtcactgtt
    28081 tctggtcctt tgctgactgt gaaagtaatc gtgtctgttt taggatcgac ttctgtatct
    28141 tctgatggag attgcgaaat gatcattccg ctttctacct ctgaactgaa ttcctcttct
    28201 ttttgaatca agtcagcaga aaaccctaat tctttcaaac ttgcttcggc atcttcataa
    28261 cttttaccac tgtaatcatc taatttgatt tttttcgtgc cgctactaat atacagagtg
    28321 acttcgcgtt tttgcttgac tgtcgttccc gctgccgggt ctgtcttcac tacattacct
    28381 tcttcgattt tgtcatctgc aatttcttct gtttttgttg ctacttctaa tttcgctttt
    28441 gcgagtgttt ctctagcaga agcttcagaa agatcagaaa catcaggaac aacaacttct
    28501 cctcgcccgc cagaagcaaa gaatacagct gttccgattc ctaaaacagc aagaatagct
    28561 aataggatca gccaccattt tttctgtcct ttctttttag gatcaggcag aacttcttcc
    28621 tcctctggta aggtttcgtt ttctggcttg tcttttggca gaggcataga tttgaatgaa
    28681 tcaggcattg gtgcatcttc cgtaaggggt gtgataactt ttgtttcatt atccatcaca
    28741 tgaggctgcc atttttcttc gtttgcacga gctggtgata atactgtaga aagatcatca
    28801 gacatttcct cggctgactt ataacggtct gcaggttctt tagccgttgc ccgtaaaatc
    28861 acgttttcca gtgcttgtgg cacatttgga tcaagtgctt ttatagatgg aagatcatct
    28921 tgaaaatgtt ttaaagcaat tgttacagca gattcaccat caaacggtac gctacccgtc
    28981 aacatctcat aaaggatgat ccctaaagca taaatatctg actgcttcgt tgccatactt
    29041 cctctggcct gctcagggga cagataatga acagatccta gcatcgtatt cgtctgcgtg
    29101 atcgatgttt cagataaagc gatagcaatc ccgaaatccg tgatttttac aacaccttca
    29161 ttatcgatca gtacgttttg gggtttcaaa tctcggtgga tgatttgatg gttatgagct
    29221 aaagaaatag cagaaaggat ctgctgcata atatttactg ctgtttcata aggaacagga
    29281 taatgtgttt ggatatatcg tttcaagtcc attcctttga cgtattccat caccagatac
    29341 tgcatgttgt cttcttcacc aacatcgtaa acactaacaa tgtttggatg aacgagttct
    29401 gttgctgcta aagcttctct ctgaaatcgt cgaatcgcag tctggtcatt ttgaaagtca
    29461 aatcgtaaga ctttaactgc tacatcacga tccaagatta aatcgtgcgc caaaaataca
    29521 tttgccatac caccgctacc gatatttcca gtgatttgat aacggccatt taattttttg
    29581 cccaactcga tcatacactg tcctccttat attcgataag aagaacggtg atattatccg
    29641 ctccgcctgc ttcattggca cgatcaatca gctcagttac tttatgagac aatgggcctt
    29701 cttgattgat gatcgttcct atttcttctt cagacagcat gttcgtcaag ccatctgaac
    29761 ataacagcag tcgatctttt agttgccaaa gatgagtaga aacatctact tcgactgttc
    29821 caggcatgcc gacagaacgt gttaaaatat ttttccttgg atggttcact gccatttctt
    29881 cgctgatttc tcctgatttg actagttcat tgactaaaga atgatcctcc gtcaattgca
    29941 tgattttatc ttcacgaatc aaataagctc tgctatcccc aacattggca atcgtgaacc
    30001 agtcatctga cagagcggcc gctacgattg ttgttcccat accgttatat tccggctgtt
    30061 cttgtccacg ctgataaatc agcatatttt cttcttgaat cgattgaatg aaccattggg
    30121 cgattttttc atcgtcaaca gcttcttgtt tttcccaagc ttcgcctaga ttcgttaccg
    30181 ccatctgact ggcaatgtct ccggcacgat gaccacccat cccgtctgct aatacagcaa
    30241 gcttgagacc tgaacggctg gtaaatacac tggcatagtc ttgatttgtg ttacgtcgtc
    30301 ttcctacatc tgattgatat tcaatctgca ttttttcacc tcattacttt ttgcgcaaac
    30361 aacaaatgaa aaatccatct gtgtaaaact gatgcggata caaagtcaac attttctctt
    30421 cgattgatga ttgtagcata ggattggtca aaacatcaat cctctcaaaa tcagggtgtc
    30481 tttccaagaa agcagaaaca acgtcttggt tttcttcttt taagatcgta caagtgctat
    30541 aagtcagaat accggaagac tttaaagttg gtgcgcagct ttctaaaatt tccaattgga
    30601 tttttggtaa attcgctaat tcatccggct gttttttata acgaatatct ggttttcgcc
    30661 gaagcagccc aagacctgaa caaggcgcgt ctactaaaat ccgatcaaac tgctctgctt
    30721 cgaactgttt actgacttct ctagcatcca tcttttgagc gtagacagca gaagagacac
    30781 ctagacgtgc tgcattatct tcaatgagtt tgactttgtg ttcgtgtaca tctaaagcga
    30841 ccacttctcc accgcatgac ggatctaaaa aagaagcaat atgcgtcgtc ttacctccgg
    30901 gtgctgcaca ggcatctaag acatgatgtt caggctcgat ttgcatagac ggcgctacaa
    30961 gcatcgaact ttcatcttgg atcgtcatga cgccatcgtg gaacaatgag ctagaagcta
    31021 aatgcccttt tttcgctact ataccgtatg gactgacttg gcttttttca gctgtgatct
    31081 cttcttcttg caaaacagtg atggcctgat ctctggaaag tcggcgtgta tctactcggg
    31141 cactagcatg gctaggctga tatagcgata aaccgagctt ttcagtttct tcgtatccga
    31201 tttgatccac cagtcgtttc gtcatccata gaggcaggct gattttgaca gaaagtcttt
    31261 cgataggatc agcaatgcct tgcagatctg gtgctccttg acgctgataa ttcctcaaga
    31321 caccatttac aaatttccct gtcccaggat ttccttttac ttttgcgatt tcgaccgcct
    31381 cgtttaaaat agcatgtgca gggactttat ctaaaaattc taattggtat acagaaagat
    31441 aaagaagcat cttgacccag tcatccactt tttttgcttt agcgatcaat ggtgcaacgt
    31501 aaaattccaa tagtcgctga tggctaatcg taccatagat caattctgta aataaaccca
    31561 catctttttg atttagctcg ctttgtttga tcatctcatt caacaaaaga ttcgagtagg
    31621 cgccaccctt gttcactcgc tctaatgtca gtaatgccat atatcgtact gattttttca
    31681 tttttttagg gatttttttc attatttcac ctgctgacca atctgaactt gctgaccgct
    31741 tccgttaagg aaatcatgta cggcctgttt tcctttacca gctggctgta attcattgat
    31801 ggctaagacg gtctgtttac cgcaagcaat ccatagattt tttttgtctt tccggatgat
    31861 cgtaccaggt gctttgtctg tagtttctgc caatacttca accgataata atttccagcg
    31921 tatgccctca taggtcgtga atgcaattgg ccaaggacgc attccgcgta cttggttgtc
    31981 aatttcttct gcagttttat gccaatcgat tgcttcttgt tcccgtgtga tgtttggcga
    32041 aaaagttgct tctgactcgt cttgaggtac tggtttttgt ccatccaaaa tattgggcaa
    32101 tgtttctagc aatagctttc ttcctaaaag gctaagcttt tcaaacatcg tccctacatc
    32161 atcttgttta gtgattggta tgctttcttg cgcatagatt ccgcctgcat ccattttttt
    32221 gatcatttcc atgatcgtta caccagtttc tttctcccca ttcatgatgg cataatgaac
    32281 aggcgcacct ccccgatatt taggaagtaa agaagcatgg acattgattg cccctaattt
    32341 tggtacttgc aataactttt caggcaagaa ttggccaaat gccgcagtga cgatcacatc
    32401 aggcaccaat tcaatgattt cttccatttc tggcgaaccg gagattttct ctggctgcag
    32461 cacacggata ccgtgcttca gagccgcttt ttttaccggt gtcggcgtaa ttactcgttt
    32521 ccgcccaact ggacggtcgg gctgtgttac cactgcctgg atctcgtaac ctgattctat
    32581 cagcccttca agaatcggca cagaaaaagc aggtgtgccc ataaatacta gcttagtcat
    32641 caagatgttc ctccatatat tcttcaagtt cttcttccgg gatctgctgt atcattttct
    32701 caatgaacaa gataccgtct aagtgatcga tttcatgctg gaaggcacgg gctaaatagc
    32761 caaaagccgt cacttctatc tcttctccgt ctcgatcata ataacgcacg gtcacttcat
    32821 ctgctcgctt gaccgttcca tagacatgcg gaatactgag acaaccttct acgtcaaggc
    32881 tttcaccttt tgcttcgatg atttctgggt taatcagttc aaatttctcg ccttcatcta
    32941 cttcgatcac agcaattctc ttgttttgtc ctacttgagg agcagcgatc cctattccgt
    33001 cattcgcaat cattgtctca tataaattgt ctaataaagc gattgtctcg tccgtgatta
    33061 catcgattgg ctgagcagtt ctttttaatt tatcattagg gtggatcaat ataggatagc
    33121 gcataatttg gccctttctt aaataaagtt taatggttcg ttgtcaattg ccacaaaaag
    33181 accttgtcgc tgttctttct ggcttccatt caaaatttca tccaaaacat gaggaagatt
    33241 cggttcatgt ttatatttga tgattagttg ataataatat cggtttttca ctctcaaaat
    33301 catgctgggc gttggcccta atagcaagct ttgagggctt aacacttctt ttagctgatt
    33361 ggcaatctga aagattttct ttgccgctac ttgttcttca ggatggctgg cggttatttt
    33421 cacagtaaag taataaggag gatagcctcc acgatgccgt acatgcattt cctgctgata
    33481 aaactgctca taattttgtt ttttagccaa ttctatggcg taatgttgag gattgaatgt
    33541 ttggatcacg acttcgcctg ctttttccgc tcgccctgct cgtcctgaaa cttgggtaag
    33601 caattggaat gtatgctcac tagaacgaaa atccggtaaa ttcagggagg tatcagcatt
    33661 caacacccca actaatgtca cttctggaaa gtccaaacct ttagcaatca tttgtgtccc
    33721 taataatata tctgcttctt ttgcaccgaa tttttgtaaa atctgttcgt gtgccccttt
    33781 tcttcgagtg gtatctacat ccatacgcaa aatcctagct tgcggataaa gttctcggag
    33841 ttcctcttct actttttgag tacctgttcc atagtagcgg attttattgc cgccacaatt
    33901 aggacagcga ttgggaatag gttcttcatg tccgcaataa tggcagcgca ttgaacgtgt
    33961 atccatatgc agtgtaagag agatatcgca attaggacat ggaagaacaa atccacagtc
    34021 cctacacatc acaaaagacg aatatcctcg acgattgagc agtaggacag tttgttcttt
    34081 tttgtctaat cgattttgta ttttttcctg caaattagca gaaaaagtag aggtccgatg
    34141 attctgaaac tcttcacgca tatctacgat ctcaatagct ggaagctgtg cagctgcttt
    34201 ggctcggtgg tttaactgca ataactgata aacgcctttt tgagctcgtg ctcttgattc
    34261 taaagagggc gtcgcgcttc ctagaactat tgggcaatga tgatatttac tgcgccaaat
    34321 tgctagatct ctcgcatgat aacgcggtgt ctcatcttgt ttgtaacttg attcatgttc
    34381 ttcatcgata atgatcagcc cgatattttt cagcggggca aaaatagcag atcgtgcgcc
    34441 tactactact tgcgcttctt cccgctcaat cttgcgccat tcatcatatt tttcaccttg
    34501 ggaaagtccg ctatgtaaga ccgcaactga ttctccgaag cgtcctttga atcgctcgac
    34561 catttgcgga gtcaatgcga tttcaggcac taacataata gccgttttat tttctgacaa
    34621 aacatcagcg attgcctgaa gatatacttc tgttttcccg ctgccagtga ttccctccag
    34681 caaaaagacc tgatcttttt gctgctgacc tgctgtgatg atttgttcga cagcattttt
    34741 ttgttctgca ttcagttcca atgccgtcgt ttggtcaaat actcggtctt tataagggtc
    34801 gcgataacgt tctgattcta taaactccag ccagcccttt tcttttcctt gattcaagac
    34861 agcagtactg attcctttat ttttgaaata agcaattggt tcttcttggc tcagttcaga
    34921 taaacacaaa agaagttctt tttgtttctt ggcattttgc cgtaattttg cccattcttc
    34981 ttcgatttgc ttttcagtta acaaagaacg gaccaaacga atcgttttta ctttatttcg
    35041 tgtatggact tcatatttga tatctactaa ttgctcttgg cgccagcgaa tcaattgaga
    35101 aaggctgcct tcttcttctg cttccttcca ggaaatcact tctcgctgat caaacaatac
    35161 ctgaacttct ggtgtgtctg ccaaaggata gatcaattta tcatattctg ctttcatcac
    35221 actcggaagc atcgtttgta gacaagtgat tttgaatgcg taagtaattt ttttcatata
    35281 atcagctaat gaaagtaatt cttcattgac caccggagca aggtctagca aacgaataac
    35341 tgccttcaaa gatggattag ctgattcttc tgatttttgg atagccataa caaatccttg
    35401 gacatgacga ttgccattac caaaagggac ttctactcgc atcccgactt gcaaagctgt
    35461 ttccatttca gagggaacca aataagtgaa tggctgatcg gtctgcatag taggtacgtc
    35521 cacaatgact tcagctgtta atgtcatggt tcgtttcctc ctttttttgt cttttcttat
    35581 caagcgacaa aaagttcctt gcctatttta ctagagatcg atggatttgt cttatcttct
    35641 gccaaatcct aacaaaaaat taacagcaga taagaaaaaa aagaactgtg tcaaaaagtt
    35701 cagacacagc cttttctacc agacaataag tggcgaatgt ttgaaaagga aacagctggg
    35761 tttgcttttc tttttagatg ttcgctctat ttttctttat tacatttttt gctcttcacg
    35821 gatacgtgat tccaattcgt gttgttcacg ctctttttgt gcttgacgtt cctcttcttc
    35881 catcttcaaa cgttcacgtt ttaattcagg atgtggatca ttgactacat ttcctgcatc
    35941 gatttcttct aaagcttgac cgacgctttt tactgaatca aaagaatcta gagtaggctg
    36001 tgcacttgca tccaattcat gcgcgcgttt acttgctaaa atcactagag aatattttga
    36061 atttacacga tctaatagtg agtcaattga aggttttaac atcataagac tacatctcct
    36121 ttaacatttt aatatattta ccgattacgc gatctactcg aaaatgttca ctggcaataa
    36181 tgtttttgat tcgttcaact gctagcggca cttcatcatt tacaacagca tagtcgtaaa
    36241 gagccatcat ttcgatttct tcttttgcaa ctttcattcg tttttcgatt acttcatctg
    36301 catctgttcc tcggcctacg atccgtgatt ttaattccgc taaatctggc ggagtcaaaa
    36361 agatgaatac accatcgggc actttttctt tgacttgttt tgctccttgg acttcaatct
    36421 ccaagaaaac atcttttcct tcatccaatg tcttgttgac atatgacaaa ggagttccat
    36481 aataattccc gacatattct gcgtattcca gcatctctcc agcttcgatc attgattcaa
    36541 actcttcttt gcttcggaag aaataatcaa cgccttcgac ctccccttca cgcattttac
    36601 gggtagtcat tgagatcgaa tactgaaaat cattatcctc actctcaaaa attgcttttc
    36661 gtactgtgcc ttttcctaca ccggatggac cagacaacac gattaataat ccacgctctg
    36721 acatgacgac gtcctttcaa ctagtctttt tgtacttatc gtttattttg cacttttttt
    36781 ctgcaacttt caaggttatc tttaattata gctagaaatt ggtaaaaaag ctaccctgta
    36841 ccaagaggtc gtaaaaaagc tgtcctgcat caagtaataa gaacagccaa acataaacag
    36901 cttctcatgt ttttcgtttg gttggggtta ttatcgcaga tgcaagcttt tgaacgccgt
    36961 gtataagagg tcgtgaagaa gccgattagt ttcgagtatg aagaaaaatt ttgtgaaaat
    37021 agtttctcac atttttgcat aaatttggct taataccgag aaaatggttt ttgaacatca
    37081 cttattcaaa ataaaaaaag attgtgacac gaattttgtc acaaccttta aatgtattcc
    37141 tagagcaaag aagctaagcg aagaaatcca attctgttta tttattgtat ttctttgttc
    37201 agcatccttg ctaaaagccc atattggaca aagtattcac tacacccatg atctcatctt
    37261 ttcgagaacg tgctttttct gtcggatctt ctacctcttc tgtgtcaatt cgaacaataa
    37321 atttccgatt catcgtcacg taagcaccgt cgttgatcaa aataaacagt tcgttctcat
    37381 tatagtctgc acaggcatct ttccatacgt caaagccgtt tttttcagaa gggatatccc
    37441 gtttaaccac agtttttcca cttaattcgt ggaacgttac gtcataatat ttcattatct
    37501 ctacctcttt cgtcattcaa tattttgtac ttgttctctg attttttcta agatcgtctt
    37561 agactggacg acttgatttt taatttcgat tgcatttgat ttggaaccga tcgtgttgat
    37621 ttcccgattc atttcttgga tcaggaaatc taattcccga cctacaggac attgtgcttc
    37681 gataagttca tctagttttt gtacatggat cgccagacga tccagttcct cgtgaatatc
    37741 tccgcgttcc aacaagatgg ctagctccgt caacaaccgt tgctcatcga ctttcgctcc
    37801 aagaaattcc tccagctttt tttgatacct tatttgaaat tccttttcat agatttctac
    37861 aaaactatcg agttttttta gtacttcttt gaattcttca gtattctttt tcaacactaa
    37921 agctaaagct tgaccttctt tttctcgact cgcctcaata gaatcaagtg cgcttttcaa
    37981 tgtatctaga gctaactgtg ccaattcttc catgctctct tccggttttt cttctaccgt
    38041 tacgaagttt tcgtccagca cgaagcgttc cgccatccga ctaattgaca cttgattttg
    38101 actaccgtat cgttgttgaa ttccgtctgt caattgagtg ataagtgaat caatcaattc
    38161 ccagttgatg acgatctgtt tttcgttttt gccaatataa gctagattca caaatacttc
    38221 gattcgaccg cggcttaact gctgtttaat aagctgacga aaatcgtttt caacaaagtt
    38281 caggatttta ggacttcgta tttgaacatc tagaaaacgc tggttgacac tttttatttc
    38341 aatatctaac tgatacgctt ttgtttcgcg gatggctttg ccaaaaccag tcatactttt
    38401 cattcgacag ggtccttcct cattacatat tttctattgc tttttgcagt tgttcaaact
    38461 cttcgttcgt caatgtcagg cctttcccca ttttttcatg gtctggcgcc caatctcgaa
    38521 gatcgaattt tggcgggcga ccattccagc tgactaggtt cagttcttta cgccacccct
    38581 tgttgttttc tgacaatata gcgatttctt ctacgatttc atatgaaaat tcttgtgcca
    38641 tggttgttcc tccttgagat aatataaata taggatcagt aatgtgtcaa ctggttgctt
    38701 aagcaattca tgctgcaaac gatccttgcg ttttccttgt agcagataat caaaataaac
    38761 agttttcgtg cgctcatttt gtattttttg ctggtatttt cgataccttc gccattcttc
    38821 agtacttaat tgaccaattt gtgccaataa atcatccggc atacttttta tatcttgttg
    38881 gcagatcatt tgaatcatca tgtctacttg atagatttca ttgggcagta aaaaatgttt
    38941 ttttgctaaa agttctagca tcgccaatcc gcatgtctct ggtacgaccc tgttcttata
    39001 taaattacgc agtttttgcc aaaattttat gcttttgaaa aaagataatt ctaactgaat
    39061 ttcttttgtt aagtaagaca aaaatcctcg ccagtatgtt cttgcaggtt taacagaatg
    39121 aaatgtatac cagttcccat agtaatagcc taatcgtttt ttcatttcca gatagcctaa
    39181 ttgtaaattt aattggttcc tttggctatc aaaaagcaga aagctgccta gtgtccacaa
    39241 tgtttgacat ttccaatgga taaaaatgtc tggcatacga atttttttcg ctgggcctgg
    39301 cccttgcaca tctacgacaa atgcctctgt tgctcctttg ttaatcgcaa tatcaatagg
    39361 tattttgttg cgatacccac cgtctgcata tttatttttt ccgatctttc gataagccat
    39421 agcgggataa aatgaagcag aggcgagtat ccaatctggt atttcttctg tttttgtctg
    39481 ttggatatca gtcactactt ccgctagttc tggcagttta gtagacaccg tatataaacg
    39541 tattttggga ttatgtttga gtaaggacaa atccaaattg tctttgacca tcttttccaa
    39601 tggagcagaa gaaaccccgc cttcaattag tgctgttttc gtcatttggc gtctttctcg
    39661 atagaagcgt tcacgaaggt cttcaactgc agccatttct ggtaacgcaa gcacttgtct
    39721 tgtcgacagc tttttccata aattcacagc ttttttcata tctccttgta aaatcaatgc
    39781 gccgttcaac gcacctaccg aggtacctgt gataatctca aagttgatat catgttcctt
    39841 cagcgcctgc cacacaccaa tctgataagc accgtgcgct cctcctccac ctaaaacaag
    39901 tgctgtatga tagaccagct ttttatttaa ttgtgtggtt ccttgatagc cgtttttatg
    39961 aaaaatacgc aaccattctt ctgatagagg agattgaaat aaaaaacaac agttttcttt
    40021 aataaaaaaa cgagcgcaag cttccatata ccgaaaaaat tttttccatt caaaagtttc
    40081 accttcaatc aacagattat taaaaataac atctcctcta cttttagcaa tagtaaaata
    40141 gacggtatat tttttcgata ctgctttaaa agcacattgc ttttttgcag aacgccaaaa
    40201 agcgtctcta ctttcttgat gactttgaaa aaatggaagg ggttgttttg cttgctggat
    40261 aggcgaatac agcgacattt tctgtgaaaa gtaaactttt tttatctcca tcgcgctctc
    40321 ctttttcttc aagtatacca gtacgtcata aaaaggtcat gagtctcgta ttcgactttg
    40381 tagattccat aagaaaacaa ttcataaaac ctcctttaaa taaaaaaata caaaaatcac
    40441 aaaaattaat tgaaaatgag agtgttttca atgaaaacgt gattttgtaa tggaatttta
    40501 aaataaaaga aacaagcttt tttaatgcaa atgttgtaaa ataaaagaga ttaagaggaa
    40561 ataagaggag gaacatgatg actgagtcag tttatgtaca tattgatacc acaagtaatg
    40621 ctgttttaac aaaagggctg actgctactg actttgcaaa cagtatcgtg cattttcctc
    40681 aaaacttgct cttgcttgat ccatctgctt ctgctggaga atatgaatcc catacagggc
    40741 tgaaagtaat ccgtggtaca gaaaatattc agcgtttctt ttcctcttct cgtaacaggc
    40801 gtggattcga tgaattgaag tggatcgact ttactgattt aacgatgctg aaagaattga
    40861 cacctttaga aatttcagaa ttattatatt ttggtcatat gaaaacacat cttcattcgc
    40921 cgttttttta taaactacag aataattttg tatacttcga tcttaatgat cagctgaatc
    40981 ggatttatta tcgctattta gatgaatttt ataggatttt tgctagtaaa ttaagttcga
    41041 tcttgtcaga taagatcaat gtaaaaaaat cttttttcaa aaaagaaaca cctgttgaaa
    41101 aaatcagtgt ggaattattg aaagaaatgc gtaatttgat gcaggaaggg atcatttttt
    41161 cttttgatca agttggtttg atcgatggta aatatcgtat cccaatctat gtgattgaag
    41221 attatatttg gaaattaaaa ggcacccggt atcgagaaga ggaaatcatc gctactttaa
    41281 tctactctgt caccgatcga cactggcaat tggtagaaga tgccgatctt tcttatctgc
    41341 cattgaagta gatctatttc ttatcaaaaa ataaaaagga aacaagaaac taggacatga
    41401 ttctgtctca agtttcttgc ttctttttta tttacagctc ctgtcgttta tctggcacaa
    41461 aaatcgaagg agtcgtttgt tggatcccta ataaaacgat gatcgttaca atagctgtac
    41521 tcaacccgct ggcaccattc attacgaaag aaaagagcca aggattcatc ccccatagtg
    41581 catacgctcc ccagaaaacc actcctgcaa taaaatgcca aaaatatctg gaaagtgcgc
    41641 caatcaatgc accaaacacg atgctttttc taatttgttt cgtattatcg tctacatagc
    41701 ctttttggac atttgaagcc atgagccctg ctgcgcctgc aaaagtatag gcaatcgggt
    41761 actcaatcaa cacttgaacg acacttaaat aataaacttg tcccaacgga aaatgtagga
    41821 tgccccaaat gaaacctgcc agcatccctg cctttgtccc tcttcttaac gcaaaaagag
    41881 tcaatggaat ctgccctaat gaaatagaaa aactgctgcc gatctggatc ggaatcaacg
    41941 acaacaccat cgctaacgct gcaacaatcg ttccttctac ccaaactcta ttttttccca
    42001 taaaaaaact tcctcctttg aataaaaaag tcacaaagga ggaagcagct ttcgaaaata
    42061 gtcaaacagc tgtttattca caattcctac gctcgcataa ccgatacagg tacgaagggt
    42121 ttagaaatag attcaatctc agcccaattg gactcccctt tgtgagttta tttattattt
    42181 attttttgtt tcaaacaatg gactaactgg tttgttttca tggatacgtt tgattgcatc
    42241 tcccattagt ccgccaacac ttacttcatc gattttgtcg attttacgat cttctggaag
    42301 atagatcgag tctgtaacaa ctagacgctc gatagctgaa tcttctattc tttgtaaagc
    42361 agggccggat aatactggat gtgtacatga agcatacaca ctagttgcac cagcttcttt
    42421 caaagcattg gcagccaatg tgatcgtacc tgctgtatcg atcatatcat cgatcagtac
    42481 acaagttttg cctttgactt gtccaatgat attcattact tctgcaacat ttgcttttgg
    42541 acggcgtttg tctatgatcg caatcggaga tttcaaaaat tccgctaatt tacgtgcccg
    42601 tgtgacacca ccatggtctg gtgaaacaac gacaacatca tcgcctttga ttcctttttc
    42661 taagaaataa ttggcaataa gtggcgcacc catcaaatga tcgactggaa tatcaaagaa
    42721 accttggatt tggactgcat gaaggtccaa agtcagcatt cttgttgcac cagctttttg
    42781 aatcatgttt gctactaatt tcgctgtgat tggttcccgt gcacgtgctt tacggtcttg
    42841 acgcgcatag ccataataag gcatgactac gttgattgtt tttgcacttg ctcgtttcag
    42901 tgcgtcaatc atgatcagca actccatcaa gttatcattg actgggctgc tcgttgattg
    42961 aataacatag acatgcgatc cacgaatact ttcttcaatg tttacttgaa tttctccatc
    43021 actaaattga gtaacagatg attttcctaa ctcgacgccg actgctgctg caattttctc
    43081 tgctaatggg cgattggaat ttaacgcaaa aatttttaat cttgggtcaa agtaatgttt
    43141 tgacatgggg acctccactt tctttttcac aactctagaa ttctacctaa taaatagtag
    43201 tcatattttt taaataaatc aagtgatttt gcaaaagtct tagtttagat aagggagttt
    43261 ttttgcatat ccttctttat tgacttgtct tgctctcgca attgccagcg catattctgg
    43321 aatattgtct gtaattgttg acccggcagc aatcgaactg tttttagcta cctcgacagg
    43381 tccgatgata ttcgtgctag aaccaataaa gctgtggtcg ccgacagtcg tatgatgttt
    43441 gttctttcca tcatagttga cgaagacaac accacatccg acattgattt cttctcccag
    43501 cgtagcatca ccaacataag tcaagtgacc gactttagtt cctttgccga tttgagcatt
    43561 tttcacttcc acaaagtttc cgatatggac gttttcgcca atttcagctt ttggtcgtaa
    43621 atgtgcataa ggtcccacat ctgcatgttt cttgacatgg ctgctttcaa tgactgagtt
    43681 ttcaatgacg acgtgatcct caatcctact gtctacgatt tttgaatgtg acccgatgac
    43741 acaatcagaa ccaatgactg tatttccttg gatttgtaca cctgcttcga tcaatgtgtc
    43801 tggaccgatt tctacgcctg catcgatgta tgtcgttgct gaatcgacaa agcttacgcc
    43861 attgaccata tgcatctggt taattcgctt tctcatgatc tcatttgctt tagctaacgc
    43921 gatccgatca ttgactccca ttgattcttc aaaatcatcc gtttgataag cagcaactgt
    43981 atggccttct tcttttaaga tttcgatgat atctgttaga tagtattcac cttgagcatt
    44041 gtttgttcca actttgctta acgcatcgaa caaagcttcg ttgtcaaaac agtaagttcc
    44101 tgtattgatt tcttgtacaa gagcttcttc tggtgttgca tctttttgtt ccacgatttt
    44161 ttctacgatc ccaatatggt cacggatgat tcgtccatat cctgttgggt tttctgcttg
    44221 tgccgtcaag atcgttgcac ttgcattttt tccttggtga tattcaaaaa gattgttcag
    44281 cgtttcagta gtcaataatg gtgtatcgcc actgataact aacgttgttc ctttttttcc
    44341 ttccaagaaa gaagcagctt gcaaaacagc atgtcctgtt cccaattgtt ctgcttgtaa
    44401 cgcatattta ctgcgttctc ctagttgtgc tttaacttgt tctgctccgt gtccaacgat
    44461 cgtaatcact tcatctggtt tcgtttcact gacccgatta atgatatgtt ctaccatagg
    44521 tttaccagaa acgggatgta aaactttata taattttgac ttcatgcggg tacctttccc
    44581 tgccgccaaa ataattgcat aacgtgcgtc caactgcgtc actcctaaat atagtttgct
    44641 gatttcagtt tatcccagta tacccatttt ttcaacaaaa caagtgattt tcttttatcc
    44701 cagcgttttc tggataaaaa aatagataga ccataacggg cctatctatt tggtaaccgg
    44761 tgctctcctg ccaactcctc ggtcacaagt cacactactc ataaaacatg aaaagctgtt
    44821 ttttgaaatc gtttcttgtg acttagagtt aaacggtagg cttacaacct cttcattaaa
    44881 cgtttttcaa tcagctgacc ttcggtatta gctcaataaa cggcaaaata tgaggagcaa
    44941 tttttccgtt tattgagcta atactcagga caggacgctg atttcacaac ctctagataa
    45001 acggtgttca aaagccagtt tctcggtatt aagccaaatt tatgaaaaaa tgtggaaagc
    45061 cattttcaca aaatttttct tactactcga aactaagcgg ctttttcaca acctcttatt
    45121 ctgaaaaata atttcctggc acaaccgtaa tatttttcgt ttgagtatct acatctttga
    45181 cataaagcag tgacgtataa tcatcgatgg cgcgatgacc gttgaatttt gattcggcaa
    45241 atacagtgat gccaacaagt tctgattcga attcttcaat caagctcttc attccgttga
    45301 ctgttccgcc gcctttcatg aaatcatcca ctacaaggac acgtgaacca cgtttcaaac
    45361 ttctcttaga cagttccatc ttttcaattc tttcagaaga tccagacaca tagttgacac
    45421 ttactgttga tccttctgtt atttttgagt cccggcgtac aataacgaat ggcgcattca
    45481 gataatagga cacagcttgg gcaataggta cgccttttgt ggctacagtc attacagcat
    45541 cgattttttc accaaggtat ttagaagcaa tgattcttcc aacttgacgc agcagttcag
    45601 gttctcctaa caaatcagag agataaacat atcctcctgg aagcagacgg tcttgttctg
    45661 atagacgttc tgctagagat tcaatatatt ttttcgcctc ttcatatgaa atttcaggga
    45721 tgaacaatac accacctgca gcacccggga ttgtttccaa ggttccatac cctcgttctt
    45781 taaatgtttt cttgatgatt cccaaatctt cactgataga agatttagca gattcataac
    45841 gttcggcaaa gcttgttaag gaaatcaatt catgcgggtg attcaataaa tactgtgtca
    45901 tgtcaactag acgttcactg cgacgtactt tcacctattc caactccttt taagaaattt
    45961 tatcgtttta cattatataa gtttttcttc aaaaattcga gcattttatg aaaaaataag
    46021 aaaatagttc ggtatttatt aaaatcgccg attgtaaaat taagctagac aactaaaaaa
    46081 agtcatttgt gctacactca aaatagttcc gacgaaagaa ttataaggag tgagtacaaa
    46141 tgacctataa ccatcttaca ccaactgaac tcgtaatgat agaagcttat ttcaatcaat
    46201 cacaacctgt ttcaaaagta gccaatctgt tacaacgttc tcgacaaact atttataagg
    46261 tctatcgttt tcttaaatct ggtggaagcg caatgagcta ctataaacaa tataagaaaa
    46321 acaaacgtaa ctgcggaaga catccgattg ttttacctga tgagcaacag gaatacattc
    46381 aaaaaaaggt tgttcaaggg tgggctcctg atgtccttat tggtcgagca gagtttccta
    46441 ttgcttgttc ggtacgcaca ctgtatcgaa tgtttaaaga taaaaccttt gacactcacg
    46501 atttaccaat gaagggaaaa agaaaaccaa acggtcataa agaaaaaaga ggaaaacaag
    46561 ggtttcgccg gtcaatccga gacagaaaga cagactatac ccagtttgat catgaatttg
    46621 gtcacctgga aggtgacact atcgtgggtg gaaaacataa aagcgcggtc atcacattag
    46681 ttgaacggct atctaaagtc atcattacct tgaaacccga aggcagacag gctaaagaca
    46741 ttgaaaatag gctgaatcaa tggtttcgtt gtgtcccaaa aaacttattt aaatccatca
    46801 catttgattg tggaaaagaa ttctcaaatt ggaaaaagat tagtaatacc aacgatattt
    46861 caatttattt tgcggatcca ggaacacctt ctcaacgagg attgaatgaa cattcaaatg
    46921 gcttattacg taaagatggt ttgtataaag ctatggattt taatggtgta tctgaaacct
    46981 ttatccaatc ggtggcatcg aaacgaaatc atattcctag aaaatcatta cactatcgga
    47041 caccaatgga agtatttttg agttacgtca caaaagatga tttgtctaac ttaatttgac
    47101 aaacgaaaaa taaaaagaag cctataaaaa atttttcgtt tttataaggc ttcttttctt
    47161 tataatttag ttattttttc ttctgaaggc aaccaatatt cgtttgatga tatttacaat
    47221 caggaacaaa gcaataaaga ctaatgtgat tgttgctcct ggaggtgtat ctagataaaa
    47281 cgagctagtt agtcctgaca gcatccctag aaatcctaca caaacactga tagcaatcac
    47341 gacattgaag cttttgccta atcgcatacc gattgctgcg ggaagaacca tgattgcgga
    47401 tatcaaaagt gcaccggcaa tcgggatcat cacagcaata gccacaccag taatcacatt
    47461 gaacatcatc gacatcaaat gaacgggtag cccatccacg tgggcggtat cttcatcaaa
    47521 agtcagaaca tacatcggcc gtttgaataa aaagaacaat ataccgatgg caacgaataa
    47581 gattcctaaa aagatgactt gctcccatgt aatggtcacg atcgaaccaa acagatagga
    47641 ttggatactt gttgctgaat tccctccgct caaattcatc aatactaacg ctagcgccaa
    47701 accaccagcc attaaaatgg caatggagat ttctgaatag gttcggtaga tcgttcgcag
    47761 atactccaat atcacagctg ctaatacaac gactagcatg gtcgtaagtg taggattaag
    47821 gttcaaaaag aatcctaacg ctacccctgc taaagagaca tgagaaagcg tatcagccat
    47881 cagcgattgt cttctgatca ctaaaaacac acctagcatc ggggcgattc cggcaatgaa
    47941 gatagcggca agaaacgctc ttctcatgaa ttcatatgaa agcaacgcca ttgagaatcc
    48001 tcctttctca ccagtcggat ctgtctgtcc gcatactgtt tgatatcttc atgatcatgg
    48061 gtgatcatta gaactgattt cccatggaca tgcgcactat gtcggagaag acgataaaat
    48121 tcatttctgg attcttcatc catacctgtt gttggttcat caagaacaaa taagtcaggg
    48181 tcagtagcaa aaatacgcgc taaactgatt cgctgctttt gtccgccaga caattcacct
    48241 attcgccgat ttcgcatatc ccacatccct acagcttcta aagcacgctg tacatgttga
    48301 tgatcttttt cagagagacg cttgaaccag cggcctcttg gatatcttcc tgaacggact
    48361 aattcgatca ccgtactagg aaaacctgca ttgaaagatg caacttgttg aggaatataa
    48421 ccgatactta atttttttcc ttcttgattt ttctcagcaa tctttatcat tccttttgaa
    48481 ggtttcagta atcctaacga tgctttcacc aaggttgact tggctgctcc attttctcca
    48541 gttaaaatga caaattcacc cgcatctaca tgataagaga catcttctag tacaggttca
    48601 tcatcatagt aaaaggtcaa atcagatatt tcgatataac gcaccttaca acctccattc
    48661 taaaacttaa ttgatacttt cttttaaagc tttcagattt ttttccatga cagaaatata
    48721 attttcgcct gctttgattt gttcattagt caagctttct aaaggattca acacttctag
    48781 cttcactcct gtttcattcg acaatgtctc agctaccttc gaagaggcat tttcttcaaa
    48841 ataaatcact ttgatgttgt ttttcttcac aaattctttt agctcagcta agcgacttgg
    48901 tgtgggttcc tgatctggtg aaacgcccga aatcgctact tgttttaagt cgtattgatt
    48961 cgctaaataa gcaaaagcag catgctgagt gacaaatgtg cggtttgttg catcttttag
    49021 ctcatcggag tatttttgat ccagtgcttc aagctttttg atataagcat cggcattctt
    49081 cgtaaaagtt tcctttttat ctggatattt ttcgcctaat tcttcagcaa tcgttctgac
    49141 ttcttttata gctaataccg gatcgagcca aacatgtgga tcgtattcat gtgaatgacc
    49201 atgcccttct tctgattctt catgttcgtc tgcttcttgc gattctaaac ggtcgattcc
    49261 tttagctgct tcgatcatca aggtctgttt gctatccact gaatccttta acgaatccac
    49321 aaaaaattcc atgtctgaac tattatacac gaaaacatct gcatcggaaa tttctgctct
    49381 ttcttttgca cttggttcga aatcatgcgg ctccgttcca gcagggatca aaagtttcac
    49441 gtttccttca tcccctacga tctcttttgt aaaatcatac atcggataaa aagtcgtaac
    49501 gatcgtcaaa tcctcttttt tgtctgcttc cttgttcgta tttccacatg ctgcaaacag
    49561 taaagcacct atcattaccg tagctgtcaa aagatatttt ttcatttata tgctcctttc
    49621 aatgaacaaa tcggaatttt acgatttatt aacgtgaact aatgtatcaa aataaaaaaa
    49681 cacagtcaat agaaatccat caatttctga tttaagcatg tatattcttg attaaatcgt
    49741 aaaatggatg agttgctgct catatctagc catttcacta caataaaaaa ctgaagaata
    49801 ccattttagg attcctcagt ttttcatcac ctattttact tcttatattt tattgcaggg
    49861 ttcgtactaa ataaacttcc tcacaaaaac cttttaaccc attatatact ctctgcgctc
    49921 ttgaataatg ttgacacaag gcaaaaacgg ttggtccgct tccactcatc aatgcagcat
    49981 ctgctccgta cttgatcata cgctctttga tttgatggat gactggatgc cgctttcccg
    50041 ttatatcttc taaactgttt cccatttttt tgatcatctg ctgataatct tgctctcgga
    50101 tggcttcagc aagtccgtgg atgtcttgat gttggatggc ttgcgtatct acttctctaa
    50161 acactgtacg tgtagagaca ctcattcttg gtttgaccaa cacaacccag cactgtggca
    50221 tagaaggtaa tacttggatt tcttcccctt tgccaccgac aaatgccgta cttccgtaga
    50281 cacagtaagg aacatctgta ccgacttcca aaccgatttc tgccagttct tctaacgaac
    50341 agcccagatt ccataattgg ttgatccctc ttagagcagc tgcacagtct gtacttcctc
    50401 cgcctaatcc agctgctact ggaatattct tcttaacaaa gatcttgaca ccggttttga
    50461 tattgtattt attttttagc aataaagcag cttgataaac attgttccgt tcatctaccg
    50521 gtaaaaatgc tttatttgtt tctacaatga ttttgtcttc tgttatttct tcaaaaaata
    50581 gatgatctgc caagtctacg ctcgacatca ccatttccaa ctcatgatac ccatcttgcc
    50641 gtctatataa tacatccagt cctaaattta tcttggctgg cgctttttca atcaattcca
    50701 tcctctcacc cattcgaaaa gtctaagtcg caagtattct atcataggga aaaataaaag
    50761 tatagcttat gtctgcctag tggttgattt tgtcatcgtt tcaacagctt tcacagaatt
    50821 ttctcttcat aatccaaaca agttctagtg attcttataa ttctgtgagc acttcttctg
    50881 ccaaactcgg aacatttcct ttttcttttc ctatcagact gagtagctgc atggtagcaa
    50941 atccgaaatt acttcctttc ctataagcaa agtaacgtcc ttcccaatga tcgacatatt
    51001 ttaatttctt ttcataagca gactggaagg aatagaaaaa attaccataa ttatatacga
    51061 tatgcatgac tttttcttga aagaaagaaa aatctgtttc tccaacatta gctaatggag
    51121 ctaagcctag attggcttcc gagtagccca ttttctcgta tacaataaac agattggcta
    51181 acagatagtc agctaattcc tcagattctt taggtaagac ccgcaacaaa tcatatcccg
    51241 cctgcttctt cgtcatcggc tgagcggtga tgaagccaac gactttttga tctttacgga
    51301 caataccgac accgctagat aatacatatg atttatcaaa tcgtcccaca gagaagtatt
    51361 tttctcgtgc accttgaagc catttttgtg aaacttcatt tacagcttga tgtaattctt
    51421 ctggcagttg atcgtaccaa gtaaaatggt atccttcttt catcaaacgt tgatattcaa
    51481 gttgctgact cagccgagac aagtcttctg ttgctagatc aacaatccct gcttcaccta
    51541 ctttcgtgaa gtgaaaaccc aattcatgaa gcatcactac gtagctttct gatatcttat
    51601 aaaacgcaag ctgatagcct aaacaatctg cttgtttcat aaactgattc gttgctgccc
    51661 gcatcatttt aggatttcca actggatctc ctaaaacaaa gcatttgttt cctttgatct
    51721 gataaccaaa aacgacttga tcttggtcat cttcttggta ataaaaataa ctgtaacctg
    51781 acaagcgaag ctgatggctt gtagctgtgc ctccgtatgt ttccaataaa cggtcaaaac
    51841 ggtctttttc gtactcactg ccaagcttcg tagtatcttc agctaaatat tgataaagcg
    51901 ccactaaagc tagcaatgag atgcccaaac caataagccc tgagaaccac acatcatctg
    51961 aaggaaacaa aataaaatgg tttcctgtta ttcgaccgac taaaaagcgt ccgctgtgat
    52021 agcctgcaat tgcgtaaacg ataaacaaaa agccgaacaa gctagcatca aagattatag
    52081 ctccccaaga ataaacgaat tttgttcgat aaaattcttt tctggataac cacacagcta
    52141 acagaatcaa gacatatacc aatatcaact gccaagataa cgtacgtgtg attgtattta
    52201 aaataccaaa agatagtaaa gcaatcgtag gcagaaatgc ctttttgact tttactgaaa
    52261 gtgctctagc aaggccgagt aataaaaagc caaccaatag attcactgtc tgatctaaaa
    52321 aattgaatga gaacggcaat aatacttgaa atagccgact gattgcagac agattagtga
    52381 ttgttgatag aagtaccatc atgattcccg caaaatagac agcacttacg agcacgatat
    52441 gcgcaacctt ttggaaaaac agtcgcggaa tgttatctaa aaatcgattg attttgactc
    52501 ctgtatgtgt gatgaacaat agaagtcccg tcataaaagg tagaacataa taaaacaacc
    52561 gataatataa tagccaaact actgctacat cttgccgaac agacaattga ctcattccta
    52621 agatcatcaa aacgtcaaaa gtccccattc cccctgggac catcgtcagc attcctatca
    52681 atgtagcaat catgaacata ggatagatcg aaagcatcga aatatctatt ctcatcaact
    52741 tgccgatgat caaaaatacc gcaagggctc ccgtccattg tccaaaggat gccagtagca
    52801 gaccaagcaa gccttttgga taaaactcag agaacaaggt ctttctcttt agataagcaa
    52861 aaaagaaaat gactggtgct aaaaaactac cgccgaaaag ccagatccaa tagcttcgaa
    52921 aaatactttc tgactgaata aaaaagacat caataaatgt aagaaacgac catacagaca
    52981 aaccagtcag cataaatagt gcgacttttg atacagtggc taaaaccatt tttctatttg
    53041 tctgctttcc ataaaaacta gctcgcaggc ttgctcctac cacaccacca aaaccggcga
    53101 gattgttgat cgtgtttgtt acccacgcag caagaagaaa ctcttttctt tccatttttg
    53161 gttttccttg tttttctaag actctgatcg tcacccaatc atacaacaac atcggagtga
    53221 cacccagcag tccagccaga atcatgagca ggatcgtcat gcggctttgt tgtcccatcc
    53281 tttgccaaat atcttgccaa ctcatgccat tagcaatatt tgcgacttga tttgccacaa
    53341 agactaacac ggagccgaaa aaaatcagtt ttaaaatcag actatgttcc tttatccatt
    53401 gcagcatcaa gtatcgttta tttttcacat tttcccattc ctaacttgta tacttttgtg
    53461 tcatacccca atgttatctt atttatttta atttttccac tttagacctg ttctttgctt
    53521 gcgatgatag aaaaagaaag atatcctaat gctaaaggag aggataacat gaaagtagga
    53581 tttatcggca cagggaatat ggcacaagct attattgatg gtttattaaa aaaaggaaca
    53641 gtctcgaaag aagaggtgtt cgtttccagt ggacattttg aaaatgcaca aaaatttgct
    53701 gctgaaactg gcgtccatgc atgcaaaagt aatcaggaag ttgcatcaca atcagatatc
    53761 gttcttttag ccgtgaaacc aaaaatcatt gaaaaagtat tggaagaact aagagaatat
    53821 agccagaaag tcttattcat ttctattgct tcaggaaaat cattaataga tttagaaaca
    53881 gcgcttgaag atcctcaggc ctccatcgta cgtgtgatgc cgaatgtcaa tgtcagcgta
    53941 ggagcaggcg tttctgcgat ttgcaaaaat gaagctgcca cggaggaaca gcttcagcaa
    54001 gctacgatga tttttgaagc aatcggcagc gtctatcctt tagcagaatc agatttcagt
    54061 acattcattg gccttgctgg cagctcaccc gcattcgttt acatgctgat cgatgcgatg
    54121 ggacggactg gtgtactgaa tggtttgtcc aaacagacag ccactgagat tgctgcaaaa
    54181 gcaattatgg gttcttgtca aaaattcctt tcaagtgata aaaatccatg ggaacttgta
    54241 gaccaagttt cttctcctgg cggtacaact gttgcaggtg tagttgcctt agaagaagca
    54301 ggattcatcc cagcaatcat caaaggaatc aatacgacga tcgagaaaga tcttgagatg
    54361 ttgaataaaa aataatgata gcaatgtttt ttcgtgcctt acaaaaaaag aagagctgtg
    54421 gcttttgccg cggctcttct tttgtatttt ttattattta gcataatcag ttgcacgagt
    54481 ttcccgaata acgattactt tgatgtgtcc aggataatcc agttcttctt cgattcgttt
    54541 acgaatatca cgtactaaac gaacagcgtc taaatccgag atttcttctg gcttgaccat
    54601 tacccgaact tcgcgtccag cttgaactgc gtaacttgat tctacaccgg cgaagctgtt
    54661 cgaaatattt tccagacttc ttaaacgacg gatgtagttt tctaatgatt cacttcgcgc
    54721 accaggtctt gctgctgaga gcgcatctgc tgcagctaca aggacagaga taacagaagt
    54781 agcttccaca tctccatgat gagaagcgat cgcgttgatt actactgggt tttctttgta
    54841 cttggcagct aattccgcac cgatctcaac gtgagatcct tcaatctcat ggtcaagtgc
    54901 tttcccaata tcatggagca acccggcacg ttttgctaat tggatatctt cgccaagctc
    54961 tgctgctaag ataccagcta attttgcaac ctcgaccgaa tgcttcaaga cgttttgtcc
    55021 ataactagtt ctgaatcgca gacgtccgat gatcttgatt agatctggat gaagtgtatg
    55081 agcaccgact tcaaacgctg cctcttcccc gtattcgcga atacgttcat ccatttcttt
    55141 acgagatttc tcgaccattt cttcgatacg cgctggatgg attcgtccat cttgtatcag
    55201 tttttccaag gtcatccgtg cgatttctct tcggattgga tcgaatccgg aaagaacaac
    55261 tgcttccggt gtatcatcga tgattaaatc gataccagtc agcgtctcaa gcgttcgaat
    55321 attacgacct tctcgtccaa tgatacgacc tttcatttca tcattcggaa gggtaacgac
    55381 tgaaacagtc gtttctgaaa cttgatctgc tgcgcagcgt tgaatcgcta atgataataa
    55441 gtttttcgct ttgcgatctg cttcttcttt ggcacgctgt tcagattctt ttaccattag
    55501 agttaactca tgattcagct cttcttcagt cgatttcatg atgacatctc gtgcgtcctc
    55561 ttttgataaa ccagcaattt tttctagttc tttttgttga tcttcaatca ttttttcaac
    55621 gtctttttct cgctcttcaa ttaattgctg ttttgaacta agtgcttctt ctttactttc
    55681 aagtgagttc tcgcgttttt ctaacgagtc atctttacga ttcagcgttt gttcgcgttg
    55741 aatcaaacga ttctcttgag attttaattc taacttactt tctctcagct cactttcaac
    55801 ttcagaacgg tatttctgat tttcttcctt agcttctaac aatgcttctt tttttagagt
    55861 ttctgcttct ttttttgcac tatcgatgat tccagcagct gatgattgtg caccagctat
    55921 ttctttctcg tgacgtgact ttgcgaccat aaaccctaaa ccaagaccga caattaaacc
    55981 gatgatagcg aggagaatgt tgaataccat aaaatccacc tccatactat ctttttcatt
    56041 tttatcatct atacagcttc tatgataaat tattctaaac tcgtcaatat gcatcttcgc
    56101 atatatgtgt acaaaaaaca tgcacaactt tattctaatg tttgtacccc gtagtgtcaa
    56161 ctttaaataa acaaagaaac ggctttctac ttattcatta aacgatggtt tttaaaataa
    56221 atgacactag tttcatatag gtaaaccttt aaattgagaa acttttctat taaattattc
    56281 tttgtagatg aacagatatt tttattttat gtacccactc gtataattaa ataactcacg
    56341 atttctttat tcaataaaaa aacggcaggt caaggagagt acctactctt cagaccagcc
    56401 gtttttgttt atcataattt ttattcttct actaaaggca attcttcttg tcctttttca
    56461 tcttctggga cagcgacttc ttcaccaata ccataagctg ctctaactag agcagaaact
    56521 tctgccatca tttcaggatg attagccata tagattttag cgttttcccg tccttgacca
    56581 atccgatctt ctttataaga ataccaagca ccgcttttat caacaatgtc tttttctact
    56641 gccatgtcta gaagttctcc ttcttgtgag atccctaaac catacatgac atctacttca
    56701 gcgattttga aaggaggtgc tactttgttt tttacgactt tgatctttgt acggttacca
    56761 acaatatctg taccttgttt caattgttct gcacgacgta cttccaaacg gatcgttgcg
    56821 tagaatttca atgcgcgtcc tccaggagtg atttctggat tcccaaacat cacgcctact
    56881 ttttcacgga tttggttgat gaaaatagcg attgtttttg ttttgttgat cgaaccagag
    56941 agtttacgca atgcttgaga catcaaacgt gcttgtaacc cgacatgtga gtcacccatt
    57001 tcgccgtcga tttctgctcg tggaactaaa gcagcaactg aatcgactac tacgatatct
    57061 acagccccac ttgatactaa agcatcagcg atctctagac cttgttctcc tgtgtcaggc
    57121 tgtgaaagaa gtagttcatc gatattcaca cctaattttt gtgcatattg cggatctaac
    57181 gcatgctcag catcaatgaa agcggccgtt ccgccatttt tttgtacttc tgcaatagcg
    57241 tgtagtgcaa ctgttgtttt acctgaactt tcaggtccat atacttcgat gatacgtcca
    57301 cgggggtaac cgccgacacc taaagcgaca tctaacgcaa gagagccact tggaattgta
    57361 gagatttgtt ggtcgatttt ttcgcccaat ttcataatag agcctttacc atagctcttt
    57421 tcaatttttt tcagtgcagc atctaaggct gctttacgat catctgccaa tacgataatc
    57481 ctccttatat ttacaaatca gagcaacatt tcattatcct tatcatatct tttttagtga
    57541 aaaaaagcaa gaaaaaaccg aacaaatatt cgcatttatc attttcttaa aagctgtttt
    57601 ctcaaaagat caaatccttg cataaccgca ttatatctga tagcagcacg atctctggca
    57661 aagtgatatt tttccgaaac cacgccgtct ttagatgaaa tagcgatcca tactgttcct
    57721 gcagattgac cttctaacgt gtccggtcca gccactccgg tgaaagctac actgtaatct
    57781 gttcttgcta tctcacgtgc ttgaatggcc atctgctcca cacattcttt gctgactgtt
    57841 ccatattctg ctaatagctc cggatcaatc tccagaaaac cagcttttgt ttggagcgag
    57901 taggtaacga atccgcctgg aaatacttca gatacaccag caatgtcgcc taatgcggct
    57961 tggaaggcgc cggctgtcaa actttccgct gctgttacag tctgcttgtt ttctttcagt
    58021 agttccacta cgacttttgc tagcgaatta tcatcgccgt atccataaaa atattcccct
    58081 actcgttctt ggattttttc ttctaatgct aaaagagctt gattccctgc ctgaacatca
    58141 tttgttttca ctgtcagtcg taatgtcact tcatttggtt tggcatatgg tgcgatcgtt
    58201 ggattgatct gtgtctcaat cagatctttt agctcagtga ccagtcgcga ttcaccaatc
    58261 ccataaaagc gcaaaacttt agaaattagt ttttcttcag aaggaaaacg ttcctctagt
    58321 aaaggacgta cttgttccac aaacattggt ttcaactcat taggcggacc gggaagaagg
    58381 atatacgcat gtgtatctgt ttgataaaaa gtccctaaag caagccctgt acgatttggt
    58441 agaggaaccc cgccttcgat aatctgggac tgttgcagat tgtttttggt catttttcga
    58501 tgggttgttt caaaatatgc caataatttt ttgtatcctt ccgtattttg tatgagagac
    58561 ttaccgaggt gagccgcagt tgcttctttt gtcaggtcat cttctgtcgg tcctaatccg
    58621 ccacataaga gtatgagttc acttcttgtt tcagctagag caatcaactc ttctaaacgc
    58681 tgctgattat ctcctacaac tgtttgataa taaacttcga tacctagatc agctagctgt
    58741 tctgacaaga aagtggcatt cgtattcacg acttgtccta gtaaaagttc tgttccgaca
    58801 gctattattt ctgctttcat tacttcccgc tcccttatat agtatacgct gtatttttat
    58861 atttttttat ttaaaaagag ggcaaggagc tgtgacaaaa atcttgtcac agctccttgt
    58921 tccaaataaa cagtgttctc ctgccaaaac ctcggtcata agtcacaata tacataaaac
    58981 atgaaaagct gttttttgaa attatccctt gtgactcaga gttagacggc aggctcacaa
    59041 cctctccact aaacgatact tacattgatc ctttgaacac accgctgttt ttgatgaagt
    59101 aatccacacc tgaatagata gtaaagatca gacaagcata aagcatgatt tgatctactg
    59161 gaataccgat caggctgaat gggatattat tgataaacag caagataata gcaaccattt
    59221 gtgtagccgt cttgactttt cccggccaag ctgcagccat gacttctcca tgttcgacaa
    59281 gtagtaaacg caagcctgtg acggctaatt cacgacagac aatgatcgct actacccaac
    59341 caggggcctt tccttggtca accaacatga taaaagcagt cataactagc attttatctg
    59401 ccaacgggtc cgcaaacttg ccaaaattag tgacaaggcc tcgcgcacgg gcaatttttc
    59461 catctaacca gtctgtgata ctagctaaag caaaaataac tgcacctact agctgtgtga
    59521 cagccagtgt ggtgtctcct acgttaaatt gtccccaatc taaaggtaaa cctacaacca
    59581 aaatgaaaat cgggatcata caaattctaa gtacagttaa tttatttggt aaattcacac
    59641 tatcgactcc tttcttccct attctacgtg actatcagcg aagtttccac cgatattatt
    59701 tacttttcca aatgaattta ttcaaaggag tatttttatt gaaattgatc tttttgaatt
    59761 aacgaagaag gaccgcgaca cagatttgtc acgatccttc ttttttatac ctacaagtaa
    59821 acagatgcgt aaactaattt atacgatcat tctgcgtatt gaagagtaag gttcacattt
    59881 tttggactct gattgttttc ccctggatta actggtacgg attctccatt cgctttgatc
    59941 gttgcattgc ttgctgcacc aatcgtgatg gtcgcatttg ttgctgtttc cggtaacacg
    60001 gtactctgtg tttcatttgc tgccagtgtc ccttgataaa cataggcatt gtctactagc
    60061 acacctaacc atacacgatt gacagcagta aattcaaggg ttacaggctt ggtagcgtct
    60121 gtgatattta ctgcgatggc actgccagaa tcttctcctg tagctattgc cattttcttt
    60181 tcttcacttg attttgttgt tgattctact gtagaagact ctttggtctg cgtgctttgc
    60241 tctgaggaag aggactcctc gactgatcct tctactttaa gcgaagaagc tgtgtctccg
    60301 attattggat cagcttgacg atcttgccaa gtcatatagc ccacgacaac gacaatcgcc
    60361 aaagcaacca atcctaataa aatgacagga agacttgtcc aaaagcgact tggatgcgat
    60421 tcttcctgat gcattgcttt tctagaagta cttactgttt caggctgcgg acgttcaggg
    60481 accgatcctg tcaatttgct tttcccatca aaaacatcga ctaaatggtc tccatcttct
    60541 cctacagctt cagcgtattg ccgaataaat gcccgcacgt aaaatttacc aggcaattgg
    60601 tcaaaatcat cattgtcgat tgcttccaaa taacgttttt gaatttttgt aatctgctgt
    60661 aattcatcta aagatatttt tttattcagg cgtgcttgcc gtaatttttc tccgatggtt
    60721 tcaaaagcca ctcgttcatc ttctccagtc tgtttgattc tccgaagcat ctgctgttgc
    60781 aacgaaagac gttatgtttg ggtgctgtga caaaagtcat gtcacagctc cttttttccg
    60841 aataaacatt gttctcctgc caactcctcg gtcacaagtc acaatatcca ataaatataa
    60901 gaagctgttt tttgaacttg ttccttgtga ctcggagtta aacggcaggc tcacgacctc
    60961 tttcttaaac ggtgttcaat cagctgttcc tcggtattag ctcaaaaacc ggcaaaatat
    61021 aaggagatat ttttccgtat gtttctctaa tactcaggtc aaaacgctga tttcacaacc
    61081 tcttccttaa acggtgttca aaagcttgca tctgcggtaa taagtccaac caagttaaaa
    61141 acattaagaa gctgttttta cttggctgtt cttattactt gatgcagggc agctttttca
    61201 caacctcttg gtacacggtg ttccagaagt agcttcttcg gcaataagcc gaaatatcac
    61261 aaaaatttga gaagcaattt tcgtaaattc cttcttattt cttgaagctg accacttctg
    61321 tcacaacctc tttgattact tcgtttgatt ttattttaat ataagttgat taaaagagag
    61381 catgatttgt tttattttct cactaatttt tccaacacaa ctaagaatac cataaaagct
    61441 gattttggag gaggttccta ctacaagtct aaaacgaaat ttaagacttt tcctcataaa
    61501 atactggttt attttggtct catgtaaaac tcgctgaagg cctcttcact tataaatgct
    61561 tccccagctg ccagtacgtc tttcatttgg atcgaatcga tgatttcagg aagatcaaat
    61621 aaagttcgat cgccaaataa actttgcgta aattgattgg caatatattc gatcgaattt
    61681 aatgattgga aatattgtcc aagcattttc ttcttcagca aatcgagatt tgtttctgac
    61741 acttccggat catcagcgaa tccaagaatg atttctttga ctttttctgc tgctttctct
    61801 ggttcgtccg tatctccgct gaagtccgca aagtgaaatt cacgatccaa gctgaattca
    61861 aaaccaaagc tgtcatcgat aattccttga ttgtacatcg ctagatagtt tcgagaagta
    61921 ttgcctaaaa tcatttggaa caacagattg attgctgttt tatagcgtaa cagttctgtt
    61981 ccttcttgcg gcaatgtgtc taatccttta ataccaagta caaatttatc tcgtgtaatg
    62041 gctgcttcca atgcactttg tttgatgatt tcttttgtgt tttcagggaa ataacgtaca
    62101 atttcttgct ttggagggaa attttttgct tcttggtttt cccgaataag tttcatcaat
    62161 ttctcaggct ccatcttacc aactacaaat aaaaccatat tgcttggctg ataaaacgtc
    62221 cgatagcacg tatacaagtc ttgagctgtg attttgtcga tactttcaac cgtgcccgca
    62281 atatcgatat gcaatgggtg tgtaggatac aagttattta gtatgccaaa aaacatccgc
    62341 cagttagggt cgtcttcata catttggatt tcctgaccga tgattccttt ttccttgttt
    62401 acagtttctt ctgtgaagta aggtgcttgt acaaaatcga tcagagtcgt cagattcttt
    62461 tctacctgat ccgtcgtaga aaacagatag ctggttttcg taaaacttgt gaaagcattg
    62521 gcggaagctc cttgtttccc aaataattga aagacatcac catcttcttt ttcaaacaac
    62581 ttatgttcta aaaaatgggc aattccatct ggaactttca ccttttcttt ttcaccatag
    62641 ggaataaatt cgttatcgat cgaaccataa ttcgtactga acaaaccata tgtcttgtga
    62701 tagtcatttt taggcaacaa atacacggtc aaaccgtttg gcagtacttc atgataaagt
    62761 gtttcattga tttgctcata ttctgtttta ttcatttgca tttcctcctg ctaaaaagaa
    62821 gattgcctgc aattcgattt gctcagctac ttgttgtatc tctttgatcg ttacgctctc
    62881 catacgtttc aaccattctt catcagataa ttttgtttcc ggcagccagc tgtccaaaaa
    62941 agaagcttcg attgccgctt gcggagagtc tagagaaagc agatattggt ttctcagcat
    63001 tgcttttgtc tgagacagtt ccaactctgt tatttcacca tttcgcaaac tttctaattg
    63061 ctcgtgaatc agatgaagta cttgatttcg gttcttttca tcgataccgg tttggacact
    63121 catgaatcct cggaacgtat ccacgctact agaggcataa taagctagac tttctttctc
    63181 tctgacattc atgaaaagtt tagagtgagg aaaaccgcca aataaaccgt taaagaccat
    63241 tagcgcaaaa cgttctggtt catcgtaata cacatttgtc tgataagcta ggttcagctt
    63301 tgcttgaacg attggctcac gtacttgtcg ttccttcacg atattcactt gaggttgggt
    63361 ataaaacaga tctggataaa ctgtttcccg cgactcaaat ggtaattgcc caactgcttc
    63421 agctgctttg tccggatcaa catcgccgac aacaaaaatg tcgatttgat catttgccat
    63481 catttcttga tagtaggacg ctagcgaact agcagtgatc ttgtccaaat cttcgactac
    63541 gcccaaccct gggatctttt gttccgggct gttttcaaaa taaagttctt gcaaggcata
    63601 ggaagcaaaa gtttgtttat cttccttcaa actttccaaa tatagacgca gattgttctt
    63661 ttccaagtca aacgtcaact gatcaaactg atgattttta atatttggat aaaataaaac
    63721 ctcttttaaa aaatctaccg cttgagaaaa aaggtttgga tcattgacat attcaccatt
    63781 cacaaaactg atccctgcgt ttagccaatg taaatttcct ttttttctaa ctgataatcc
    63841 aaagcttgcg ccataaagct ctgcaagttg cgcacttaac tttgtctgat ccgaataatt
    63901 caaactattt gtttccaaca tgctgctgag aagtgaacgt ttcgtgatga cttcttgttg
    63961 cagacgcgca gtaaaacgga taaaaatccg gatcgttttg tattgttttg ttggtaagac
    64021 atgaagattc acgccttctg ccaattgata caccatattt atatgctcct tttcacagtt
    64081 gattgcccgc ttactctttt aatgggtcaa cctgcctttt ttacttattc ctctgcttga
    64141 aatttataag ttcaggcaat tcgccgtttt ttctaaatga tgttacactt tatagtatag
    64201 catagatgtt ttttatcaaa agaaacagac ggctaaaagc gtatattgtt tttttatttt
    64261 ttatttttcg atatactaaa gacaaataaa agaagggagg gtcattcatg atcaaagaat
    64321 tcaaagagtt catcatgcgt ggagatgtct tggatcttgc tgtcggcgtt gttattggta
    64381 gtgcgtttac aagcatcgta aaccaagtgg tgcaaggctt gatcacacca ctaataggtt
    64441 ggtttgtcgc aatgatcaca ggtacttctg atttagaagg agcactttca attcttgact
    64501 ggtcgccgac aaaaggcgtc acatttgcgt ttggtaacgt tattagtgct atcattacat
    64561 ttctgatcac tggttttgtc ttattcttag ttgtcaaagc ggcaaaccgt gcgaagaatt
    64621 tacgctccaa agaagagcaa gtggaagtgg aagaaactcc tactgcagaa gattatctta
    64681 gtgacatacg cgatcttctt gccaaacaag tggcagaaga caatacacat ccagtagagc
    64741 aaacaaatct ttccgatact tctaaataat aaagaatcta aaaaaaagaa agccgatttt
    64801 cagtgtcggc tttctttttt tagattctta acttgctact tgttcctttt tcatgtcgat
    64861 atgtggaata tcatcttcca aatacacatc tgagattgcc ttgaatccaa acgactcata
    64921 aaaagcttgt aggtattctt gtgcgccgat cacgattggt ttttctggaa aatttttctg
    64981 gatcatgtta atcgtttctt gaacaatctt tcttcctaaa ccatctttac ggttgtcctg
    65041 atgaacaagg acacgtccaa aatggatact ctccgcttct tcatatactc gcgtgtaggc
    65101 aaggatcttc ccatcagtat cttgaaaaaa cgtatgccaa gcttctccat caatgtcatc
    65161 aacttcttga taaggacact gttgttccac aacaaaaact gcgattcgtt ctttgacgat
    65221 cgcgtaaaac tctgctgttg acaattcttc aaatttcttt acctgatatt ccactaagca
    65281 aacgccccct gcttttttat tcttagcata ccgattcgca cacaaaaa
//
LOCUS       DASHHB010000010        63856 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.010, whole genome shotgun sequence.
ACCESSION   DASHHB010000010 DASHHB010000000
VERSION     DASHHB010000010.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 63856)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 63856)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..63856
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.010"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(43..288)
                     /locus_tag="U2K71_000707"
     CDS             complement(43..288)
                     /locus_tag="U2K71_000707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Veg family protein"
                     /protein_id="HEM7314815.1"
                     /translation="MPTTLASIKKDLEGRIGSPIMLVAQTGRKRQTERRGVLTETYPS
                     VFVVDLDPEENSFERVSYSYSDVLTRTVEIEFLTEAV"
     gene            complement(403..981)
                     /locus_tag="U2K71_000708"
     CDS             complement(403..981)
                     /locus_tag="U2K71_000708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM7314816.1"
                     /translation="MSEELLRAARNGDEEAFAALYQKYFPLLLRLKNTYYLRNYVSED
                     WEQEARIALYQALRTYEKSKGVSFGSYYKSVISNQIYSLLRKQNALKRRDQKDEVSID
                     QKIETEGPDFLAEITLQEPLAIQHLLLKEAIENCEIQFSKIEAKIFHYYMQGVDFVDM
                     AECLAMDHKQVMSAYGRVKLKIKKHLKFYNEE"
     gene            complement(1058..1588)
                     /locus_tag="U2K71_000709"
     CDS             complement(1058..1588)
                     /locus_tag="U2K71_000709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HEM7314817.1"
                     /translation="MKKQLLIVDGYNMIGSWPELVQLKKQDKMADAREALLHRLSNYA
                     KYEGMEVIVVFDAQLVPGIQQNYKKYQLDVVFTKEDETADSYIERIAGQLNDRLTQVT
                     VATSDLAEQWMIFSQGALRTSAYELYKTIKKTEKTIAQENKDMHFIDFRRNSPWNEEQ
                     LKKLSQKLDDLMRKNK"
     gene            complement(1592..2452)
                     /gene="rlmB"
                     /locus_tag="U2K71_000710"
     CDS             complement(1592..2452)
                     /gene="rlmB"
                     /locus_tag="U2K71_000710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HEM7314818.1"
                     /translation="MGKERTNKHQSKRGFEDKKQNRYSNKKQTGRNQSVSDQNEEALE
                     DNFVFGHHAAVEALQQGRGNKLFLQEDSRGEKVEELKEIAREQAVPVKWVPKQKLETL
                     SDHGVHQGVVLAITPYEYLSLDQLIERTKEKKEDPFFLILDSIEDPHNFGSILRTADA
                     SGVDGVIIPKHRAVGITPIVTKTSTGAVEHVPVARVTNLVQAVQQLKETGFWVFGTDM
                     TGTNFQKWNAKGSIALVIGNEGKGMSPGLKKEMDELLTIPMTGHVQSLNASVAAGLLM
                     YQAFSQRQGE"
     gene            complement(2445..2840)
                     /locus_tag="U2K71_000711"
     CDS             complement(2445..2840)
                     /locus_tag="U2K71_000711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mini-ribonuclease 3"
                     /protein_id="HEM7314819.1"
                     /translation="MRDYTQLNGLALAYVGDAIYEIYVRDHLIDQGETKPNKLHRAAT
                     NYVSANAQAFLIQQMLEEHLLTEEEELYYRRGRNAKSHTSAKNADVTTYRVATGFEAL
                     MGYLHLTKQTERMEELIRWCIQKVGEKNG"
     gene            complement(2837..4249)
                     /locus_tag="U2K71_000712"
     CDS             complement(2837..4249)
                     /locus_tag="U2K71_000712"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HEM7314820.1"
                     /translation="MIKIYNTLTRKKEEFIPIQKGHVGMYVCGPTVYNYIHIGNARST
                     IAFDTIRRYFEYRGYKVDFVSNFTDVDDKIIRTANELGITAPEVADRFIRAFEEDTKA
                     LNVEPATLHPRVMDHMPDILAFIQVLVEKGYAYESSGDVYYRTRKFENYGKLSDQSID
                     ELEVGASQRTGEEQQQKEDPLDFALWKQAKPGEISWDSPWGKGRPGWHIECSVMATKH
                     LGETIDIHGGGQDLEFPHHENEIAQSEAKTGKKFANYWMHNGYVTIGEDDEKMSKSLG
                     NFITVHEMIKNVDPQVLRFFMATTQYRRPIRYSETTMKEAATNLQRLKNTFDNLKFRQ
                     ETASENLADDADKIALLENLEQRFIEEMDDDFNAANGITVVYELAKWLNTYLDQPKVS
                     AAVLAQGEALFSQWLSIFGILFTAEELLDEEIEQLIEERNQARSNRDFVRSDEIRDLL
                     KEKGILLEDTPQGTRWRREA"
     gene            complement(4246..4782)
                     /gene="cysE"
                     /locus_tag="U2K71_000713"
     CDS             complement(4246..4782)
                     /gene="cysE"
                     /locus_tag="U2K71_000713"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HEM7314821.1"
                     /translation="MSWFTRAIDAAKRNDPAARSTAEIVLTYPGFHALFWHRFSHFLY
                     QHRLFLLAKINAQFWRFLTGVEIHPGATIGTGVFIDHGMGIVIGETAEIEDDVILFHG
                     VTLGGTGKETGKRHPTVKQGAMLSANAQILGPVTIGKNAKIGAGAVVLKDVPDDATAV
                     GVPAKVVRIKGEKVRRSE"
     misc_binding    complement(4868..5107)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(5104..6564)
                     /locus_tag="U2K71_000714"
     CDS             complement(5104..6564)
                     /locus_tag="U2K71_000714"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HEM7314822.1"
                     /translation="MTKVRVRYAPSPTGHLHIGNARTALFNYLFARHNDGDFIIRIED
                     TDQKRNIEDGEKSQLENLAWLGMNWDESPENPGEYGPYRQSERKEIYQPLIDQLLASN
                     RAYKCYCTEEELEAEREAQRARGEMPHYAGTCANLTPEEQAEKEAQGLESVVRFRVPR
                     NTEYAFTDMVKGAISFESDNIGGDFVIQKRDGMPTYNFAVAVDDHLMKITHVLRGDDH
                     IANTPKQLMIYEAFGWTPPTFGHMTLIINSETGKKLSKRDESILQFIEQYRELGYLPD
                     AMFNFIALLGWSPVGEDEIFEHQELIKMFDPKRLSKSPAAFDAKKLQWVNNHYIKAMD
                     LDAFTDMCLPYLIADGRVAENPDEKTIEWVKKIVSLYQPQMSYAAEIVDLSTLFFNEH
                     PVLNEAAKEVLAGETVPTVLHAFKAQLEQMEVVDAPTVKAAIKAVQKETGVKGKNLFM
                     PIRVAVSGQMHGPELPETIELLGREKSLAHLNQVLN"
     gene            complement(6734..7891)
                     /locus_tag="U2K71_000715"
     CDS             complement(6734..7891)
                     /locus_tag="U2K71_000715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN/TRAM domain-containing protein"
                     /protein_id="HEM7314823.1"
                     /translation="MQKRVITLLMIIVGASLGISFLPSAWQAIGQQSNGWLNNTFTNS
                     LLGALIFFLLSLLLAKHISAIIKQVEQKLSEVSLTYLLFGAIGAIIGLTLGVVISTPL
                     YNMEIPFVNSVLPILLMIILGYLGLRMGTTRIEEWKKVFGSRSKKIEQETASQVESQL
                     LERKSGENFHKYKILDTSVIIDGRIYDITKTGFLEGTLMIPNFVLYELQYIADSGDSL
                     KRVRGRRGLDILNALQKEEGISVEMYDGDFEDISEVDSKLIKLAKLLDGVIVTNDYNL
                     NKVSEFQNVPVLNINALANAVKPVVIPGETMNVLVVKAGTERQQGVAYLDDGTMVVVE
                     DGQHYMNERIEVVVTSALQTAAGRMIFAKPAHAGRGIDNKKETEKKTKQSK"
     gene            complement(7925..9295)
                     /gene="radA"
                     /locus_tag="U2K71_000716"
     CDS             complement(7925..9295)
                     /gene="radA"
                     /locus_tag="U2K71_000716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HEM7314824.1"
                     /translation="MAKKSKVQFVCQNCGYVSPKFLGRCPNCGKWNTMVEEIEQDTTD
                     RRTRTSLTGEKAKPTKIADVVPKKEPRIKTKLEELNRVLGGGVVPGSMVLIGGDPGIG
                     KSTLLLQVSQQLAAIGGKVLYVSGEESAEQIKLRAERLGSINTEFYLYAETDMNEISR
                     AIEHISPDYVIIDSIQTMTQPDITSVAGSVSQVRETTAELLKTAKTNGIAIFIVGHVT
                     KEGSIAGPRMLEHMVDTVLYFEGEQHHSFRILRAVKNRFGSTNEIGIFEMHEHGLEEV
                     ANPSQIFLEERLDGATGSAIVVAMEGTRPILVEIQALVTPTMFGNAKRTTTGLDFNRV
                     SLIMAVLEKRAGLLLQNQDAYLKAAGGVKLNEPAIDLAIAVSIASSYKEKGTQPTECF
                     IGEIGLTGEIRRVSHIEQRVKEVQKLGFTKVYLPKNNLGNWEAPKGIEIVGVATLAET
                     LKRVFR"
     gene            complement(9358..9885)
                     /locus_tag="U2K71_000717"
     CDS             complement(9358..9885)
                     /locus_tag="U2K71_000717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dUTP diphosphatase"
                     /protein_id="HEM7314825.1"
                     /translation="MRKRGFEVISKYKDQNINLPQRATHHAAGYDFEAAEEVVIPSIW
                     KAGWKKGLETVADGLKKDVSENEKQLKPTLVPTGIKAYMGEDEFLQLANRSSNPLKRF
                     LVLTNGVGVIDSDYYNNEANEGHIMFQFTNFGFKDVVIKKGERIGQGIFLPFLKADQD
                     QTTAQRQGGFGSSSK"
     gene            10115..10921
                     /gene="proB"
                     /locus_tag="U2K71_000718"
     CDS             10115..10921
                     /gene="proB"
                     /locus_tag="U2K71_000718"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HEM7314826.1"
                     /translation="MRKEIKNAKRIIVKVGTSTLIYPNGNINLAAIDQLAFVLSDLRN
                     RGKEVILVSSGAVGVGMHKLHLSERPTLIPEQQAAAAVGQAELMNIYNQRFSVYNQQT
                     AQILVTRDVVDYPESRKNVRNTLEQLLIMDTIPIVNENDTVAVDELDHLTKFGDNDQL
                     SAIMAKLVAADLLIVLSDIDGFYSDNPTTNPEAQMYRYIDKIDEKILAQAGGAGSTFG
                     TGGMQSKLKAASRIFEANSAMILANGKNPAIIFDILAGKEVGTLFKEDLK"
     gene            10918..12165
                     /locus_tag="U2K71_000719"
     CDS             10918..12165
                     /locus_tag="U2K71_000719"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HEM7314827.1"
                     /translation="MTDLMQLGQQAKDSAQQLALMPTNRKNELLVQMAQTIKKNQQAI
                     IEANHKDLEKAVENNISETMQDRLQLTPERINAMATEIEKIASLDDPIGKVDEMWTNT
                     DGLRIGKKRVPLGVIGIIYESRPNVTTDAASLAFKSGNAVILRGGKEAFFSNQLLVQL
                     LQQVLVSAGESPYAIQFVDDTSHETAGQLMRLTEYLDVLIPRGGAKLIQRVKEQATVP
                     IIETGTGNNHVYIDKEAQLSMAVNIIVNAKASRPSVCNAAETLLIHSEIAPFFLPAIE
                     KELVEHGVSLRADARALEYLETAALAEDTDWDTEYLDYILAVKVVDSLDEAIAHINRH
                     NTKHSETIVTDSYAASQRFLNEVDAAAVYVNASTRFTDGSVFGFGAEIGISTQKLHAR
                     GPMGLNELTSTKYIIYGDGQIRE"
     gene            12241..12585
                     /locus_tag="U2K71_000720"
     CDS             12241..12585
                     /locus_tag="U2K71_000720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314828.1"
                     /translation="MFEILMIFFIYLISLNIAAFLGVGILSLFFQFKKRSIGSQREKW
                     SQYFDKIGQKGLVTRLHISYMVALCLLATINYYSFFDHSIAYTITLLIAGIFHLSYKY
                     QLNKNHLNRTFR"
     gene            complement(12702..13028)
                     /locus_tag="U2K71_000721"
     CDS             complement(12702..13028)
                     /locus_tag="U2K71_000721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314829.1"
                     /translation="MLEEYFQEVGLTLVELPIEFSDDRLIYRGYIKELPIINAEGETR
                     KAMQQKIAGMYQVYREQLLAESAEEEKEKTMNLSMDELLRYYDGETFDGFSLLNEEET
                     DLPGSW"
     gene            complement(13643..14845)
                     /locus_tag="U2K71_000722"
     CDS             complement(13643..14845)
                     /locus_tag="U2K71_000722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7314830.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
     gene            14962..15477
                     /locus_tag="U2K71_000723"
     CDS             14962..15477
                     /locus_tag="U2K71_000723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM7314831.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            complement(15676..16122)
                     /locus_tag="U2K71_000724"
     CDS             complement(15676..16122)
                     /locus_tag="U2K71_000724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEM7314832.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(16169..17647)
                     /gene="msr(C)"
                     /locus_tag="U2K71_000725"
     CDS             complement(16169..17647)
                     /gene="msr(C)"
                     /locus_tag="U2K71_000725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEM7314833.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            complement(18001..18630)
                     /locus_tag="U2K71_000726"
     CDS             complement(18001..18630)
                     /locus_tag="U2K71_000726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEM7314834.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            complement(18614..19078)
                     /locus_tag="U2K71_000727"
     CDS             complement(18614..19078)
                     /locus_tag="U2K71_000727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM7314835.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            complement(19565..20833)
                     /locus_tag="U2K71_000728"
     CDS             complement(19565..20833)
                     /locus_tag="U2K71_000728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEM7314836.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            complement(21064..21801)
                     /locus_tag="U2K71_000729"
     CDS             complement(21064..21801)
                     /locus_tag="U2K71_000729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM7314837.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            complement(21868..22437)
                     /locus_tag="U2K71_000730"
     CDS             complement(21868..22437)
                     /locus_tag="U2K71_000730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEM7314838.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            22565..23437
                     /locus_tag="U2K71_000731"
     CDS             22565..23437
                     /locus_tag="U2K71_000731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM7314839.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            23779..24651
                     /locus_tag="U2K71_000732"
     CDS             23779..24651
                     /locus_tag="U2K71_000732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM7314840.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            24648..26606
                     /locus_tag="U2K71_000733"
     CDS             24648..26606
                     /locus_tag="U2K71_000733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM7314841.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(26938..27789)
                     /locus_tag="U2K71_000734"
     CDS             complement(26938..27789)
                     /locus_tag="U2K71_000734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM7314842.1"
                     /translation="MHYLVTGATGGFGGYALEFLKKMVPQNEIYVLVRSEEKGEALKE
                     AGFNIRIGDYDDLDSMKEALKGIDRLLFVSGVPGNRQAEHKNVVKAAQAAGVSYIAYT
                     SFADADHSISVLAPDHQFTEKMIKDSGISHTFLRNNWYVENELPIIGQALKTGQFVYA
                     AGNGKTGWALKREYAEAAAKVLVESDSPEILELSGNLTDYEELAKALERATGKKLEII
                     EASDAAFVENLKEAGFPQEAADMFLAIQHDIKKDQLAISSDDLEKVLGKPLTSLEAAL
                     KEILDQE"
     gene            27912..28379
                     /locus_tag="U2K71_000735"
     CDS             27912..28379
                     /locus_tag="U2K71_000735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HEM7314843.1"
                     /translation="MKYSLQFSDAIHVLAYIEMFQDTRSLSSEMIASSIETNAANVRK
                     IMSQLKKSGLIFTKTGKPQPSLAKSPDQISLLDVYRSIEGNTQLIQVDPKTNPNCVVG
                     ANIQEALEESYARLQKKAEEEMAKITLDSLIHKIAVLEKEKRPENTGLVERFL"
     gene            complement(28642..29124)
                     /locus_tag="U2K71_000736"
     CDS             complement(28642..29124)
                     /locus_tag="U2K71_000736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="effector binding domain-containing protein"
                     /protein_id="HEM7314844.1"
                     /translation="MAKYRIEEKPDFTVTGIGVELKSSNTDFVGLAAEKQAFEQKVIG
                     NGTIDALKQAAENHYFFTVNEPYNGKMMYYVGVQTSQDVPNTTRIIPFPKGTYLVVIA
                     LAESPEELRNDLTNTAFGHVLREVEDYAYVGGPNAVVEMGVTNGKYMGEIWIPVVNKP
                     "
     gene            complement(29224..30087)
                     /locus_tag="U2K71_000737"
     CDS             complement(29224..30087)
                     /locus_tag="U2K71_000737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HEM7314845.1"
                     /translation="MLKDLNQLIDYIEEHLTEGLSYKIIAENTGISEYQLKRIFTFIA
                     GMSLMDYIKNRRLALANRDLLDGSRVTETAFRYGYETVEGFSRAFRDWSGYLPSEISR
                     YGIQKSFPRLTFFIDVRGGKSMEFRIEKKEQFYVAGVAKKVPVKFEGVNQEIQKLAQT
                     ITETQREEMHKLGDLYPNQVINVSYGFDGARLEEKGSLMHMIGFATTKENPYADLEML
                     AIEEHTWAIFPNKGPFPQTLQDTWGKIYAEWLPSSDYELVEAPEISFTKWGEDFSNVY
                     SEIWIAVKAKK"
     gene            30685..31560
                     /locus_tag="U2K71_000738"
     CDS             30685..31560
                     /locus_tag="U2K71_000738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314846.1"
                     /translation="MNNWLEYFPENVLERGYSYHLHGFVRHLNYTSKYLSATVSGTED
                     YKVVITWDEKTNMTCDCLYAIEGKKCKHMAAVLFAYEERPIKKSNYSLSELSSLVSSA
                     SSSLVRELLTEILIEHPQFIERFKVKMPFHAINYSDKLTTIIHKYDHIIKKNKNRKTA
                     KFIMEMRKFIQEAVESLIQQNAYLPAFELINEVIATLETFYWEPEDERTLLLIEDCYY
                     LWKELLAEAPHAEKRQMFSWFVCQVDHTDASYSKRYSIKILKEDFREKEFSNQKKKID
                     KKTKETVKKDDFNEK"
     gene            complement(31705..31857)
                     /locus_tag="U2K71_000739"
                     /pseudo
     CDS             complement(31705..31857)
                     /locus_tag="U2K71_000739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874852.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="xylulose kinase"
     gene            complement(32125..33456)
                     /locus_tag="U2K71_000740"
     CDS             complement(32125..33456)
                     /locus_tag="U2K71_000740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7314847.1"
                     /translation="MGKSSRKDIVFEKFGIVATKLGNQIHLRTLRDAFATFMPFMMLA
                     GFVTLINSVILEPTGFMGEIVDTSTLTTLQQIGTSIANGTLSITTLLVVAAVSYHMCA
                     SRNYTNHIAAVLVAISTFVVLTPMQMMFTPENADKAVEVTGVIPISYTGASGMFVGIF
                     VGLAATELFIKLSNNKKLQIQLSGNIPPAVLKSFNVLIPIIITITSFALLSFIIIQLF
                     NMDINKLITTIVTEPLSKITTGLPGFLLITSIANLFFGFGIHQAVISGSLLDPFLIQN
                     MQENMAAYANKEQIPHIINMAFKDTFAVMGGSGNTIALLIAIFIFSRRKDYKDFAKLS
                     VTPAIFNISEPIIFGLPIVFNISLIIPFVLAPIFSLTVAYFATAVGLINHVVVQIPWT
                     TPPVISGFLATAGDWRAAVLQILIIAASVFIYLPFLRIDEHVTTKLSENET"
     gene            complement(33605..34318)
                     /locus_tag="U2K71_000741"
     CDS             complement(33605..34318)
                     /locus_tag="U2K71_000741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016611336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM7314848.1"
                     /translation="MKKIAKYMEIYLDIKKKIVNGEYKIGEKLPTGDELAVIYHTSKL
                     TVKKGVDLLVSEGVLRSRSGYGTEVVRTPIDNSRVLGPNEGLFSVVGEEHVDSEIHTF
                     SIELPSKKVADKLSISVKDYVYNIVRSRFIDCQPYSIEQTFMPLAVIPGLEPKHLKKS
                     VYAYITNELGLEIKTSHIRIKGDTANEFDAKILKIDPGEFMIEIDKVVALSSGKPFEY
                     SVSRHLYQDFVFEAVFVEK"
     gene            34493..35917
                     /locus_tag="U2K71_000742"
     CDS             34493..35917
                     /locus_tag="U2K71_000742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM7314849.1"
                     /translation="MTTMKKLTFPDDFWWGSAWSAEQAEGKGETGKAETIWDRWFKEQ
                     PYRFYQRIDSSITTDHIHQYKEDIELMKKTGHNSFRVSISWARMFPNGGTGKVNPKAI
                     SFYRKLFSEMNNNGIKVFANLYHFDMPATLQDKGGWESREVVDAYVHYAETCFKEFGD
                     LVCHWFTFNEPLGPILGTYLEDFHYPNLIDFKRGAQAAFFTILAHAKAIQVFKELALP
                     SKIGVILNLSPTFPRSQNPADVKAAEIADLFYTRSFLDPMVKGNFSKKLILLLQEYNQ
                     LPEDYTKEDLVCIYENTAQILGLNYYEPRRVKSRSSMVDTSSPFLPEWFFEPHIMPGR
                     RMNEYRGWEIYEKGIYDLCMDIKNHYGNIEAFISENGMGVADEERFMDDTGQIIDDYR
                     IEYIKDHLAYLWKAINEGCNIKGYHLWTFIDCWSWINAYKNRYGLVSLDLTTQKRTIK
                     KSGEFYKQLSDTNGFRYDVDLLVE"
     gene            36216..36923
                     /locus_tag="U2K71_000743"
     CDS             36216..36923
                     /locus_tag="U2K71_000743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEM7314850.1"
                     /translation="MDEKLDIKTAIKDTLPTVFGYIGIGLAFGIVGKAAGFHPLVVTL
                     MSLLIYAGSAQFITVSMLASHSPLLSIVLSTFLVNSRMILMSMTIAPYFKKNRLLQNL
                     LIGTLLTDESFALGMNKLNYTGQKLNFRWMNTANWISYLTWVGSSLVGALLGNFITDP
                     KKFGLDFAIVAMFIGLLYLQVISDRNTSKRLQLILIGLTLILVYVGLIFIPSNLVILV
                     VTLIGCGLGVWIKHAFF"
     gene            36910..37233
                     /locus_tag="U2K71_000744"
     CDS             36910..37233
                     /locus_tag="U2K71_000744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEM7314851.1"
                     /translation="MPSFEFTLLTILGCTLATWLSRILPFVLLKKFDLPQPLLEYLSF
                     VPIVIMSALWFSSLFTQNIGHLPQINLENALASVPTLLAAILSKSLLVIVLVGILSLS
                     LIRLL"
     gene            complement(37322..38359)
                     /locus_tag="U2K71_000745"
     CDS             complement(37322..38359)
                     /locus_tag="U2K71_000745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM7314852.1"
                     /translation="MSEKELMKVVGFYEGLSIDDPKSFIDEKEQIPVPSSRDLLVKVN
                     AVSVNPVDTKLRQDNGIRNALRILGFDGVGKVVAVGDEVQKFSVGDRVFYAGTTTRAG
                     SNQEYQLVDERIVALAPKNLSDEEAAALPLTSLTAYELLFEKFGLTPEENANRGKKIL
                     VINGAGGVGSILNQLAHWAGLEVYATASPKNFEWLKKTGVDYPIDYHHDLSKSFRELG
                     IDQVEYVAVLFDITRYFDQIKHLVRPFGHVGTIVGIKEPLDIGVWKNQSISFDWEYMF
                     AKTDYNYEIETQGEALAHIAALANEGIIRSTLSKVYSDGMNAKNLKQATKDVETGHIL
                     GKVVVSGPFNG"
     gene            complement(38680..39663)
                     /locus_tag="U2K71_000746"
     CDS             complement(38680..39663)
                     /locus_tag="U2K71_000746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA-dihydrouridine synthase"
                     /protein_id="HEM7314853.1"
                     /translation="MTTNFWKELPKPFFVLAPMEDVTDVVFRHVVKHAAAPDVFFTEF
                     TNSDSFCHPDGKDSVRGRLTFTEDEQPMVAHIWGDKPEFFREMSIAMAEMGFQGIDIN
                     MGCPVPNVAERGKGSGLILRPEVAAQLIEAAKAGGLPVSVKTRIGYTEMSEMEEWISH
                     LLHQDIANLSVHLRTRKEMSKVDAHWEVIPKIIALRDQIAPQTLITINGDIPDRQTGE
                     ELAEKYGVDGIMIGRGIFKNPYAFEKEPREHTEKELIGLLRLQLDLQDHYSEIIPRSI
                     TGLHRFFKIYIKGFPGANDLRVQLMNTKSTDEVREILDTFEKEHPTLFSEQ"
     gene            complement(40078..40848)
                     /locus_tag="U2K71_000747"
     CDS             complement(40078..40848)
                     /locus_tag="U2K71_000747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314854.1"
                     /translation="MFNLYFTALKSLAIKETNRYLRIWVQTLVPPVITTSLYFVIFGK
                     MIGGRIGDMGGFSYMEFIVPGLIMMSAITSSYANVSSSFFSQKFQKNIEEILVAPVPT
                     HVIIWGFVIGGVGRSILVGSLVTIISLFFVPLHVYSWFMVIITLLMTAILFSLAGLLN
                     GIFAQSYDDVSIVPTFVLQPLTYLGGVFYAISMLPPFWQAVSKVNPIVYMIFGFRYGF
                     LGTIDVPVATSMIVLVLFIVVLYAVTWYLINKGRGLRS"
     gene            complement(40841..41776)
                     /locus_tag="U2K71_000748"
     CDS             complement(40841..41776)
                     /locus_tag="U2K71_000748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314855.1"
                     /translation="MANALEINNLKKVYGSGVEALRGIDLSVKEGDFYALLGPNGAGK
                     STTIGIITSLVNKTSGKVNVFGYDLDTDLVRAKQQIGLVPQEFNFNPFETVQQIVVNQ
                     AGYYGVSRKEAMKRSEKYLKQSNLWEKRNVRARMLSGGMKRRLMIARALMHEPRLLIL
                     DEPTAGVDIELRREMWEFLRELNENGTTIILTTHYLEEAEMLCRNIGIIQSGELIENT
                     SMKELLSKLQFETFIFDLAPYETKPVIEGYACTFEDEQTIAVEVERNQGVNGIFDQLT
                     RQGIKVLSMRNKSNRLEELFLKITEENNHTEEKNV"
     gene            complement(42092..43741)
                     /locus_tag="U2K71_000749"
     CDS             complement(42092..43741)
                     /locus_tag="U2K71_000749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEM7314856.1"
                     /translation="MGQREFIVLIIIAAVILLVALDIFSRLKLKETVRSKWEKIPYQP
                     RFDKEESLKEAWLTEKKFRSWDSEIDDLTWYDLDMFEVFEGINSTYSSVGSEALYQRL
                     RSFDFGEDQQLEKLIAFYQENPQLREKIQYQFARLGKKDHNFAKQYLADGKSKKIGRV
                     GLFIFLGLLPLVGLGLLLLGQVLGIYLAIGGLVFNTVYYLVKKQTLETELNSMSYLVS
                     TISAANQLAKLPTPLQEELKQQTKPLRDIPKLGFSFRMKSGSEAELIFEYVNIMFMLP
                     FISYHFVLTKLEKHSTEAIKLWELLGELEVAAAILNYRTYMPITCRPEFTEGGVTAKD
                     IYHPLLKEAVVNPVNWQKNTLVTGSNASGKSTYVKSIAISCILAQTIQTAIAEQFTLQ
                     PGHVLTSMAIEDDLFEGDSYFVSEIKSIKRLLDQVASKERCYCFVDEILKGTNTIERI
                     ASSASVVRWLAEYPSLAFVATHDIELTEILKNYCENVHFEEQVTEGKGISFDFKLKEG
                     PSRTRNAIALLKVLDYPETLVETAKTEAALFDEKRQWHVLG"
     gene            complement(43858..46461)
                     /locus_tag="U2K71_000750"
     CDS             complement(43858..46461)
                     /locus_tag="U2K71_000750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEM7314857.1"
                     /translation="MVNAHIIQHTHWDREWYFTNEDAIVLSDQVFTEVLDELERNPKV
                     NFCLDGQTSIVDEYLEINPQKLKVIQRLMKTGQLFVGPWYAQTDALLVDAESILRNLM
                     IGISDTILKYGDPMMVGYLPDTFGFNANLPTLLHQVEIDNFMCWRGLNFETMVPSPYF
                     IWKGLGDKFVYAMNFPFGYMTGMMTLDAIKDIPEFAKEKLDEAVEMLHMQGKNKDILV
                     PSGVDQKSMILNFDQVVEKLNKYSKFHNIISDYPSYVEVIRNKKELPEYKGELRQPVY
                     SRVHRSIGSVRTKMKIENFKLEQKILKRVEPLMLIAERNGIHISKGLLRRLWKKILQN
                     QAHDSIGGCVSDNVAEDIFHRIKEANEIADGIENLIVKRMADALKLKSNEVLVFNTTT
                     ENYSGSKIVHVVADSKKIHFKGSPYNIIEKEVYFPERKNAYRMIATGFEYFDEPAYYE
                     LDIKINVNVPALGYTVVEFEKNNEKLETAIEIDKTEISNNKYKLSFKDGQLNLIVGDR
                     QYLDFVHLIDSANDGDTYDYSPLEGDTELSLKLETAKVYKDSLQETLVVYGKAQLPKD
                     LKDRLSEKPEMEEISYEISFSLGESQIVEGTLKIHNTVYSHRMRLAINVLDKSEKSIA
                     QIQNGFIENTPDVIPPNWSETMVEKPVNLEIFDKSVSVAENDHYFTFYADGLKEYERI
                     ENQLIITLFSTTGELGKPNLAWRPGRASGDTTNEGHVMMETPLAQEIGEYKVTFGFNV
                     EEGRFNEFKVAKQAEKRLEQSISYQKQKLNVFIHRLDNKIWPLQNELDVPRKFSLIEL
                     PEDLIVSSIYPSYFHEQGFVLRLANPTEQEKIVPEAILSLGTVVNALENKQELTTIPP
                     YDYLSILINER"
     gene            complement(46473..47522)
                     /locus_tag="U2K71_000751"
     CDS             complement(46473..47522)
                     /locus_tag="U2K71_000751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIC"
                     /protein_id="HEM7314858.1"
                     /translation="MEFKKQGREILNHFQSGVSYMIPLVVAAGLLTSIAVIFGGTGVW
                     DQTDTFWGVLRMIGQTGLQFIVPMISAYIAYSIADRPGLAPAFITGMMCQNLGMGFIG
                     GMVAGLACGYLANALKKVKVPMQVISLKSIMLIPFVTTLVVGIILWYVLATPIQAMTT
                     GLTNWLEGMSGANAALMSAILGGMMAFDMGGPINKIAYAFGVASFSSGSFGPSTAMLL
                     GIAIPPFGMFLATILNKKLYDESERDNAKTAMIMGLVGITEGTIPFAVKDPLRVIPSI
                     VVGTAVACGLNGFFGVTQETMLSTFMAIPFVTNIPLYLVSIVIGGVVTALMVNALKSI
                     KYKKEQQVNSVEEGK"
     gene            complement(47548..47862)
                     /locus_tag="U2K71_000752"
     CDS             complement(47548..47862)
                     /locus_tag="U2K71_000752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEM7314859.1"
                     /translation="MTKIVAVTACIAGIAHTYMAKANLEKFAKKKGLDIKVETQGAMG
                     IENKLSISDVSEADVVIFAVDTNVSERGRFDGKPILTVGTSEVVKDGEETIDEAIALI
                     NK"
     gene            complement(47878..48324)
                     /locus_tag="U2K71_000753"
     CDS             complement(47878..48324)
                     /locus_tag="U2K71_000753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM7314860.1"
                     /translation="MISEKININNIVIDLNASDKKSVVEKLSKLLETNGFVSDHKQFQ
                     KDVFDRENHMTTGIGKNIAIPHGKSLAVIESTVAVAKLKRSVEWGALDDQPVDLVFLL
                     AIKDTDKGTNHLRILAELSGRLMDDEFVKRIKDSRNKKELVKALSF"
     gene            complement(48325..50247)
                     /locus_tag="U2K71_000754"
     CDS             complement(48325..50247)
                     /locus_tag="U2K71_000754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antiterminator"
                     /protein_id="HEM7314861.1"
                     /translation="MGIEFSERQMKILYFLIENEHTTYKELSEQLYVSTKTISKDILI
                     LKEIIQKMPDVQMLVQPKKGVSLIGKKETLRNLLSSHKINIADSPEDRLTYVFAKLLN
                     TNDSLKIQNLADELYVSRSTVESTMKDVRKRLNKHGMSLVSDRNGVRIKADEKSKRRL
                     MSEVVNYYWGGFTASNSKTDLLDLNINFIGEANNFLNKDILKKVAELLNRYMNKTQLK
                     FTDYEFQTLAIHLAIAIERIETSNFLSRSNNSEEEISKNTLILIKMIEKAFSIIVPID
                     EQAYVDIHITSIEKNAMNHDLDQTKLSYLDMGVELQELVRIKLNEFYPDEELINNLVL
                     HLNAAVKRLKLGVNIYNPYTDKIKYSFKRSFMISVDLLEEIEERFCIHFNEDEIAYVT
                     LHIQSLLDRYKPDKTKVILVCSSGYGTSKLLEQRINNGFADMVEIEDVLSINELQDRK
                     VTDELVISTIPIENTNFPVIVVSPLMLGADVRKVKQYLQLNEQTEDAFLNLLDKRFCF
                     FTHQIKDNQVNVLNIITKKLVKENYAKEGILEGAIKREKLSSTAMNDFAMPHIEVDFI
                     KKPVICVYVNPEGIDWDGTLVHSVFFFALNEKVKPELNKIYETFNEIASNHELLANLK
                     TSSSFEKLVYILKEGL"
     gene            complement(50459..51355)
                     /locus_tag="U2K71_000755"
     CDS             complement(50459..51355)
                     /locus_tag="U2K71_000755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM7314862.1"
                     /translation="MIKWLLLLVVIFMGSFIFFLQREIKRLTKKIATLSERQEYGGRL
                     SVEFREKNLMDLVDALNTWLDENEQKGQAFQEMEENIRLSIVGLSHDLRTPLTAINGY
                     VQLLEQVEDTEKREKYLTIIKQSVARLLSMTDQFYDLARIETKQKEMDLHTLSFSKEV
                     EEVFFSFYEQFSEKNIEVAFAEHSINTTVLADPLMLLRVLQNIIQNLLRYADGQAKVT
                     FSKEDPFIRLTVANPIKPGTQTSVEKVFQRFYTENTSRTNTESSGIGLYLSKKLVEGM
                     GGEMTARLDGGIFSISVKLRRV"
     gene            complement(51348..52028)
                     /locus_tag="U2K71_000756"
     CDS             complement(51348..52028)
                     /locus_tag="U2K71_000756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7314863.1"
                     /translation="MVLLETILIIEDDEAVHSLLEEVLEQRYKLLDAYSGTEGKLLLS
                     TYPVDLILLDLMLPGLSGEALLAEIRQTSNVPIIVLSAKSDQRDKVSLLAAGADDYVT
                     KPFDIEELLLRIGIQLRHQTSVQVKDLSQRIYYKEILLDKESRDVNVNGVAVHLTGRE
                     FDLLKLFLEHPKKVFSRANLYETVWQEPYFDSEKTVNMHISNLRGKLAKAHAVYIHTV
                     WGVGFRFD"
     gene            complement(52029..52811)
                     /locus_tag="U2K71_000757"
     CDS             complement(52029..52811)
                     /locus_tag="U2K71_000757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314864.1"
                     /translation="MFNLLTAEKIKVWKSRKMWVSLGLMVVLPLFYSWNEWYMHTKYG
                     NELNQATDTVINGATGILMVEKMAAWILLAFAAFACFYIGEEFQNGTIRNTLSLGRNR
                     MTYYVSKLLVTLLITTLGVVLITGLAMLAYTLAFGFGEVEGIKNYGNYVLKVFPVLLL
                     LTLGTLSVPVALTFITRSTSVSLLLSFLYIMGTAFIPGVFAKIKGLEFLTEWFTETWL
                     MYTDFAQQATYSQAPKMILVSLVTIVVSSALGMFIFQKSDIK"
     gene            complement(52804..53721)
                     /locus_tag="U2K71_000758"
     CDS             complement(52804..53721)
                     /locus_tag="U2K71_000758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7314865.1"
                     /translation="MDFGNETVLKATGITKKYGAAKALDKVSIEIKRGMIYGLIGENG
                     AGKSTFMRTIMGLISIDEGSIELFGTTDLQAARRRMGQSIETPALYPELTARDNLRIQ
                     AANGGVSDREIEDLLKMMRLENTGKKKAKNFSLGMRQRLAIANALITNPEFLILDEPT
                     NGMDPAGMAEMREIIQRLVKERGITVLLSSHLLDELSQIATHYGILHEGHLIKELSKE
                     ELAQESRQFIKIDTSATEQAVTVLDSLGYRDYFVQSSRVIQLFEGIDQVAAINQALVE
                     AKVPVDGIHLVGQKLEDYFLQLTGGNPHV"
     gene            complement(53885..54208)
                     /locus_tag="U2K71_000759"
     CDS             complement(53885..54208)
                     /locus_tag="U2K71_000759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314866.1"
                     /translation="MFSFAFYMVWLQREVPTHIQGRLMSTITTITMVASPLGLALFGA
                     LFDWKTTQPRLRDSLIFLGGSLAIVILITGLVKLLKLDLKEAKIFSEEEYRETTLVEK
                     KESGN"
     gene            54996..58223
                     /gene="fss3"
                     /locus_tag="U2K71_000760"
     CDS             54996..58223
                     /gene="fss3"
                     /locus_tag="U2K71_000760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEM7314867.1"
                     /translation="MYKKQKKKLCVLTILSLAVQAIVGAIAPTITFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGFKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSDKAKLVSVLWKKVGTDADTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     EAKINQAIDDYQKKPTFHNSTAKTVLGQSTTVTDTNNLNLSEFDEVVENAANIDYRVN
                     GNQLIITPNANSKESGVLTLKKSAGTGTPVAYKKAGQQTLMAGAIDKPNTYTIKIDVE
                     TEGSLKIKKVDKESGDVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGTKVTIT
                     EKSVPAPYTIDTTPMTTTIKAGETIYVTSKNAREKGQIILDKSGVETGSDLWNDNYSL
                     AGNTFAIRKDSPTGEIVQEMTTDENGHAETPKEIANALELGTYYVTETKASHGFVNTF
                     KPVKVELKYANQTVAPVTSNVKGQNQEVTGETTLTKEDKDTGDKAQGKAVFEGAEYTL
                     FTAKDGKAVKWSEAFKPELAKGTKGSDETVTLTLDEKNQVAVKHLAINEYYCQETKAP
                     EGYSLDETKYPVSIKKVDDSEKNAVITRNVTAKEQVIRFGFDFFKFAGSAAGTTETGF
                     NDLTFKVSPLEGTNEITGAEDEATTAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGF
                     QKITPLEIRSTFKENKEDFVKSEYVFTITEQDQKQPIKTVTVPYEKLTNKAFSVSLNR
                     LMLYDLPEEEDSLTSLATWKDGNKELTSLDSTELVDKLSYNLHEIKEDWYVVAQAIDV
                     DATKAAQEKDEKAKPVVIAETSATLANKEKTGTWKIQHKLTAEQVLNKTIVLFNYVYE
                     NKEAFEAGDKPVAKDVSLNNQAQTVSCTVEHHVSIQTKAHLENGSQTFTHGDVVDMFD
                     DVSITHDVLDGSKEAFETILYALLPDGTNKEIWKSGKIDYEVNDKEFTKTVLAKKVDT
                     GKYPEGTKFTFAEINYDKDGNINGKHNEDLKEKSQTLTPKEVPTILSTPKQPETPTVP
                     SGSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
     gene            58336..58650
                     /locus_tag="U2K71_000761"
     CDS             58336..58650
                     /locus_tag="U2K71_000761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM7314868.1"
                     /translation="MELKFVVPDMAETFGKLSYAGEGEIITEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            58663..59037
                     /locus_tag="U2K71_000762"
     CDS             58663..59037
                     /locus_tag="U2K71_000762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM7314869.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTIGNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIVDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPNQGNPTNQNERKK"
     gene            59047..59382
                     /locus_tag="U2K71_000763"
     CDS             59047..59382
                     /locus_tag="U2K71_000763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="damage-inducible protein J"
                     /protein_id="HEM7314870.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLVKALTSDEKMFLIIHQGLL
                     GENSDSEEDSDVLDWLGEEESFFIVVEVNENLCRQAESILEEIGVEMPDAIEGFLNQL
                     VETKQLPSS"
     gene            59465..60805
                     /locus_tag="U2K71_000764"
     CDS             59465..60805
                     /locus_tag="U2K71_000764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEM7314871.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQEFRQFDI
                     SKLSLSTIEIVWSIKDLVCLLGSLAFSGAMILLYIHFFLDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTRETISYFPKICYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDMIANRIGIDEVVAENGTLRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFILDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMARSKAMKEMSNYKTGENYAYLGLPPNFLIFDEYVAYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVEKNFFMKHIKGRGYVDTGGSVISEFYTPLVPKGYDFLDSISKLEKMND"
     gene            60883..61557
                     /locus_tag="U2K71_000765"
     CDS             60883..61557
                     /locus_tag="U2K71_000765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM7314872.1"
                     /translation="MESNDSYLEYAKANKDKFISYIIKGKSPEVGRDAVFMAGSPGAG
                     KSEVAIGLAENYDNHVIIDADYFRTQFPDYNGKNSSLFQKASSWLVEQAFRYVLDHGY
                     SFILDATFAILSAEKNISRALKKHFRVTIFYVYQDPKIAWKFTQEREIAEGRHVPKKA
                     FINAFFKSRENIAKVKSKHPDVLLHIIIKDYTNNISEVHYATDNLELFLPLGYSRKDL
                     EVQLHD"
     gene            61550..61714
                     /locus_tag="U2K71_000766"
     CDS             61550..61714
                     /locus_tag="U2K71_000766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314873.1"
                     /translation="MTEKEQVTKIVKKYNKSIADLSENATAKEFKTVIKYVADQANEK
                     QRKLVGLNKK"
     gene            61790..62932
                     /gene="dcm"
                     /locus_tag="U2K71_000767"
                     /pseudo
     CDS             61790..62932
                     /gene="dcm"
                     /locus_tag="U2K71_000767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613853.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA (cytosine-5-)-methyltransferase"
     gene            62922..63212
                     /locus_tag="U2K71_000768"
     CDS             62922..63212
                     /locus_tag="U2K71_000768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314874.1"
                     /translation="MVYDELIGEIYWVIGKIQSDPELEEQLRRLKFDIRKNGVKVAGD
                     PYLMNEETDARIEINQVIAEFERIADQAKEPDIRQYLLEMKAELEVNGITDE"
     gene            63471..>63856
                     /locus_tag="U2K71_000769"
     CDS             63471..>63856
                     /locus_tag="U2K71_000769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7314875.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATACGLSRPYLNQIENGGV
                     TASIKTMRKIFDQLESFNPDLPLTLLFDYVRIRFPTTDARKIIQEILHLKFEYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGV"
BASE COUNT        19110 a        12908 c        11526 g        20312 t
ORIGIN      
        1 aaaaagcaaa cgaaccttac gaatctcagg ctcgtttgct ttttaaactg cttcagttaa
       61 aaattcgatc tcaactgtcc gagtaagtac atctgaatag ctgtaagata ctcgttcaaa
      121 agaattttct tcaggatcca aatcaacaac gaatactgaa ggataggttt ctgttaaaac
      181 gccgcggcgt tctgtttgac gctttcttcc tgtctgagca accaacatga ttgggctgcc
      241 gatacgacct tctaaatctt tcttgattga agctaaagtt gttggcatac agttcacctc
      301 tactatcaac tataccacat atttttaaaa ttttcaagtt taccataaaa aaaaaagaaa
      361 tgcaataaaa ataaataaga agctcattaa gatttttcta aattattcct cattataaaa
      421 tttcaagtgt ttttttattt tgagttttac tctcccgtaa gcgctcatta cttgcttgtg
      481 atccattgct aaacattcag ccatgtctac aaaatcaacg ccttgcatat aataatgaaa
      541 aattttagct tctatttttg aaaactggat ttcgcaattt tcgattgctt cttttaataa
      601 aagatgctga attgctaaag gttcttgcaa tgtgatctct gctaaaaagt ctggtccttc
      661 tgtctctatt ttttggtcga ttgagacttc atctttttga tcacgacgct tcagcgcgtt
      721 ctgcttcctc aacagagaat agatttgatt gcttataacg cttttataat aacttccaaa
      781 gctaacacct ttgctttttt cgtaagttct caatgcctga tataaagcga tccttgcttc
      841 ttgttcccaa tcctcagaaa cataatttct taaataatag gtgtttttca aacgtagtaa
      901 aagtggaaaa tatttttgat acagggcagc aaatgcttct tcgtcaccat tacgtgcagc
      961 gcgcaataac tcctcagaca taaaataatt cctccataaa ttcgtttatg gaggaatctt
     1021 atcatcaatg attcatcgaa ctgattagaa cgatatgtta tttatttttt ctcattaaat
     1081 cgtccaattt ttgggacaat ttttttaact gctcttcatt ccatggagaa ttacgtcgga
     1141 aatcaataaa atgcatatct ttattttctt gggctattgt tttttccgtt ttttttatcg
     1201 tcttatataa ttcataagca gaagttcgta atgctccttg ggaaaagatc atccactgtt
     1261 ctgctaaatc gcttgtcgcc actgtcactt gtgtcaaacg gtcattcagc tgaccagcga
     1321 ttctctcgat ataactgtca gccgtttcgt cttctttcgt aaaaacaaca tccaactggt
     1381 atttcttata attttgttgg atgcccggaa ctagttgggc atcgaagaca acgatgactt
     1441 ccattccttc atatttcgca taatttgata aacgatgtaa aagagcttct cttgcatccg
     1501 ccattttgtc ctgcttttta agctggacaa gttccggcca cgaaccaatc atgttgtagc
     1561 cgtctacaat caacagctgt tttttcatgg tctactcccc ctgacgttgg gaaaaagcct
     1621 gatacataag caaaccagca gcaacactgg catttaagct ctggacgtgc ccggtcatcg
     1681 ggatcgtcag caattcatcc atttcttttt tcaatcctgg actcattcct ttgccttcat
     1741 taccaatgac taaagcaatt gatcctttgg cattccattt ttggaagttt gtgccggtca
     1801 tatctgtgcc aaatacccaa aatcctgttt ctttgagctg ttgcacagct tgtaccaaat
     1861 tcgtcacgcg tgcaacaggg acatgttcga ctgccccagt ggaagtcttt gttacgatag
     1921 gtgtgattcc aactgctcgg tgttttggta tgatcacacc atcgacgcca cttgcgtctg
     1981 ctgtccgtaa aatcgaccca aaattgtgtg gatcttcgat gctatccaaa atcaaaaaga
     2041 aggggtcttc ttttttctct tttgttcgtt caatcagttg gtcaagggat aaatattcat
     2101 aaggtgtaat cgcaagaacg actccttgat gcacgccgtg atcgctcaaa gtttctaatt
     2161 tttgtttagg aacccatttg actggcacag cttgttcacg tgcgatttct ttcagttctt
     2221 ctactttttc tcctcgtgaa tcttcttgaa gaaagagttt attccctcgc ccttgttgta
     2281 gcgcttctac tgcggcatgg tgcccaaaga cgaaattatc ttctaatgct tcttcatttt
     2341 gatcagaaac tgactgattt cgtccagttt gctttttatt tgaatatcta ttttgttttt
     2401 tgtcttcaaa tccacgtttc gattgatgtt tgtttgttct ttctttaccc atttttttca
     2461 ccaactttct gaatacacca gcggatcaat tcttccatcc gctcggtttg ttttgtcaaa
     2521 tgcagatagc ccatgagcgc ttcgaaccct gttgcgaccc gatacgtcgt aacatcagca
     2581 ttttttgcgc tcgtatggct tttagcattt cttcctctgc gataataaag ttcttcttct
     2641 tctgtcaaaa gatgttcttc cagcatttgc tgaatcaaaa aggcttgtgc attcgcagaa
     2701 acataattcg ttgccgcacg atgcagctta ttgggttttg tttctccttg atcaatcagg
     2761 tggtcacgaa catagatttc gtaaatcgca tctccaacat aagccaaggc taaaccgttc
     2821 aattgtgtgt aatctctcat gcttctcttc tccatcgggt tccttgcggt gtatcttcta
     2881 ataagatccc tttttctttc aacaaatcac gaatttcgtc gcttcggaca aaatcacgat
     2941 tgcttcttgc ttgatttcgt tcttcgatca attgttcaat ctcttcatct aataactctt
     3001 ctgctgtaaa taaaataccg aagatcgaaa gccattgcga gaataaagct tctccttgtg
     3061 ccaagacagc ggcagaaact ttcggctggt ctagataagt gttcagccac ttagcaagct
     3121 catagaccac tgtgatccca tttgctgcat taaaatcatc gtccatttct tcgatgaatc
     3181 gctgttctaa attttctaac aaagcgattt tatccgcatc atctgccaaa ttctctgagg
     3241 cagtttcttg gcggaatttc aaattgtcaa acgtattctt cagtcgttgt aaattggtgg
     3301 ctgcttcttt catcgtcgtt tcactgtagc ggatcggacg acggtattgt gtggtcgcca
     3361 tgaagaaacg caagacttgt ggatcaacgt ttttgatcat ttcatgtaca gtgatgaaat
     3421 tccctagaga tttactcatt ttttcatcgt cttcgccaat cgttacatag ccattgtgca
     3481 tccagtaatt agcgaatttt ttccctgttt tcgcttcact ttgtgcgatt tcattttcat
     3541 gatgaggaaa ttctaaatct tgcccgccac catggatatc gatcgtttct cctaaatgtt
     3601 ttgttgccat aacggaacat tcaatatgcc aaccgggacg tcctttgccc catggtgaat
     3661 cccaagaaat ctcgccaggt tttgcttgtt tccataaagc gaaatctaat gggtcttctt
     3721 tttgctgctg ttcttcccct gtacgctgac ttgctccgac ttccagttca tcgatggatt
     3781 ggtcacttaa tttcccataa ttttcaaatt tacgggtacg gtaatacaca tcacctgaag
     3841 attcataagc atagcctttt tcaaccaaca cttgaataaa tgctaaaata tctggcatat
     3901 gatccatgac tcttggatgc agtgtagcag gttcgacatt caacgctttc gtatcttctt
     3961 caaatgctcg gatgaaacga tcagcaactt ctggtgctgt aatacctaat tcgtttgctg
     4021 tacggatgat tttatcgtct acatccgtga aattcgatac gaaatcgact ttatagcctc
     4081 gatattcaaa atagcgtcga attgtatcga aagcaatggt gctacgagca tttccgatat
     4141 ggatataatt atacacagtc ggtccgcaga catacatacc gacatgtccc ttttgtatag
     4201 ggatgaattc ttcttttttt ctggtcaatg tattgtagat ttttatcatt ctgatctcct
     4261 cactttttct cctttgattc gaacgacttt tgctggtact cctaccgctg tagcgtcgtc
     4321 tggaacatct ttcaacacaa cggcacctgc accgattttg gcgttttttc cgatcgttac
     4381 cggtcctaat atctgcgcat tcgcagagag catggctcct tgtttaacag ttgggtgtct
     4441 tttacccgtt tcttttcctg tgccgcctaa tgttacgcca tggaataaaa tcacatcgtc
     4501 ctcgatttct gcggtttcgc caatgacgat tcccattcca tggtcgatga acacgccagt
     4561 tccaatcgtt gctcctggat ggatctcaac accagttagg aaacgccaga attgagcatt
     4621 gatcttcgcc aataaaaaga gccggtgctg atataaaaaa tgagagaaac gatgccaaaa
     4681 caaggcatga aaaccagggt atgtcaaaac gatctcagct gtcgaacgtg cagccggatc
     4741 gtttctcttt gctgcatcaa tcgctcgggt aaaccaactc atgactttcc actcctttcg
     4801 taagaatttt atgtaggaaa tcatcttctt ataaagaaaa gaagcgcctt ttaaataagt
     4861 ttccttatca aaagacgctt tccgcgtggt tccacttttc ttcataatca atgattatct
     4921 ctgacagcta acgcctgtta tacgttcttg gctactttgt ttcctataaa acagtttttc
     4981 ctgttttctg tgtttcgcca agaccactcc cagaggcatt tcggcaacca ttcattgata
     5041 ccttgcacca cccggtatct ctcttgtaat gatcagctgc ttactttttt ctgttcatcg
     5101 tgtttagttc agtacttgat ttaaatgtgc taatgatttt tcacgaccaa gcaattcgat
     5161 cgtttcaggc aattctggac catgcatttg tcctgataca gctacacgga ttggcatgaa
     5221 aagatttttt ccttttacgc cagtttcttt ttgaacagct ttgatggcag ctttgacagt
     5281 tggagcatct actacttcca tttgttctag ctgtgctttg aatgcgtgga gaacagtagg
     5341 aactgtttca ccagcaagta cttcttttgc tgcttcattc aatacaggat gttcattgaa
     5401 aaatagcgta gacagatcaa cgatttctgc agcatagctc atttgaggtt gatacaggct
     5461 aacgatcttc ttcacccatt cgattgtctt ttcatctggg ttttctgcta cacgtccatc
     5521 agcgattaga taaggcagac acatgtcagt aaatgcatct agatccattg ctttgatgta
     5581 atggttgttt acccattgca gttttttcgc atcgaaagct gcgggtgatt tacttaggcg
     5641 ttttggatcg aacattttga tcaattcttg gtgttcgaag atttcgtctt cgcccacagg
     5701 tgaccagccc aataatgcaa taaagttgaa cattgcatct ggtagatagc ctaattcgcg
     5761 atattgttcg ataaattgca agattgattc gtcacgtttg cttagttttt tacctgtttc
     5821 actgttgatg atcaatgtca tatgtccgaa ggttggtggt gtccagccaa atgcttcata
     5881 gatcatcagt tgttttggtg tattggcaat atggtcatct ccacgtaaaa cgtgagtgat
     5941 tttcatcaaa tgatcatcga ccgcaacagc aaaattataa gttggcatac catcgcgttt
     6001 ttggatgaca aagtcgccac cgatattatc ggattcgaac gaaatagcgc ctttcaccat
     6061 atctgtaaat gcgtattctg tattgcgagg cacacggaaa cgtacaacag attctagacc
     6121 ttgtgcttct ttttctgctt gttcttcagg tgtcaagttc gcacatgttc ctgcgtaatg
     6181 aggcatttct cctcttgcgc gttgtgcttc ccgttctgct tctaattctt cttctgtgca
     6241 atagcatttg tatgctcgat tgctagcaag cagctgatca atcagcggtt gatagatttc
     6301 tttacgttca gattgacggt aagggccgta ttctccagga ttttcaggag attcatccca
     6361 attcattccc aaccaagcaa ggttttccaa ctggcttttt tcgccatctt cgatattacg
     6421 tttttggtca gtatcctcaa tacgaataat aaagtctcca tcattgtggc gggcaaacaa
     6481 ataattgaat aatgcagttc ttgcatttcc aatatgcagg tgcccagttg gacttggtgc
     6541 atagcgtacg cgtactttcg tcattttctt cctcttcttt caaactagac tttgactaac
     6601 agcaaaaatt ccgtcagtta cggcttctca aatatcattt atttaaagcc actcagcaca
     6661 aattttacct tttctaaggt aaatagtaaa gccaactggt tgaatttcct taccagttgg
     6721 ctgaatttct tttttatttt gattgtttag tctttttctc tgtctctttt ttattgtcga
     6781 ttcctcgtcc tgcatgtgca ggtttagcaa agatcatgcg tccagcagct gtttgaaggg
     6841 cacttgttac aacgacttcg atacgttcat tcatgtaatg ctgtccatct tctacgacaa
     6901 ccatcgtgcc gtcatcaagg taggctactc cttgttggcg ttctgtcccg gcttttacta
     6961 ctagaacatt catcgtttca ccagggatga cgactggttt tacggcattt gccaacgcat
     7021 tgatattaag aacaggtaca ttttggaatt cagagacttt attcaagtta tagtcattgg
     7081 ttacaatgac accatctaaa agcttagcca atttgatcaa tttgctgtct acttcactga
     7141 tatcttcaaa gtctccatcg tacatttcga cagatatacc ctcttctttt tgcaaggcat
     7201 ttaggatatc taatccgcga cgaccacgca cacgtttcaa actatcccct gagtccgcga
     7261 tgtattgtag ttcgtaaaga acaaaattag ggatcatcaa ggttccttct aagaatccag
     7321 tctttgtaat atcataaatc cgaccgtcaa tgatcacgct tgtatccaag attttatatt
     7381 tatggaaatt ttctcctgat tttcgttcta acagttgact ctcaacttga ctagctgttt
     7441 cttgctcgat ttttttgctt cgtgaaccaa aaactttctt ccattcttcg attcgtgtcg
     7501 ttcccattct caatcccaga tagcctaaga tgatcatcag taaaatcggc aatacactat
     7561 tgacgaatgg aatttccata ttgtaaagag gggtcgagat cacaacacct aaagtcaatc
     7621 cgatgatcgc accgatcgca ccgaagagaa gataagtcaa gctgacctca cttagttttt
     7681 gttctacttg tttgataata gctgagatat gtttggccaa cagcaaagat aataaaaaga
     7741 aaataagtgc gcctaacagg ctgttggtaa atgtattatt cagccatccg tttgactgtt
     7801 gaccgattgc ctgccaagct gagggtaaaa atgaaatacc caagcttgcg ccgacaatga
     7861 tcatcagcaa agtaatgaca cgtttttgca tcgtttttcc tccgtttcga caatcattta
     7921 ttagttagcg gaacacacgc ttcaacgttt cagccaaagt tgctactcca actatctcga
     7981 ttccttttgg cgcttcccag ttacctaagt tgtttttagg taggtatact tttgtgaagc
     8041 cgagtttttg gacttctttg actcgttgtt caatatgact gacacgtctg atttctccag
     8101 tcagtccaat ttcaccgatg aaacattccg ttggctgtgt tcctttttct ttatagcttg
     8161 aagcaatact gacagcgata gctaaatcaa tcgctggttc atttaatttt accccacctg
     8221 cagcttttaa gtaagcatct tgattctgaa gaagcaaccc agctcttttt tccagcactg
     8281 ccatgatcag agatacccga ttgaaatcaa gacctgttgt tgttcgtttg gcatttccga
     8341 acatagtagg tgtgactaat gcttggatct ctaccaaaat tggacgtgtc ccttccattg
     8401 ccacaacgat agcagagcca gttgcaccgt ctaggcgttc ttctaagaaa atctgtgaag
     8461 gattagcgac ttcttccaat ccatgctcat gcatttcaaa aatcccaatc tcattcgttg
     8521 agccaaatcg gttttttact gctcgcaaaa tccgaaaact atgatgttgt tcaccttcaa
     8581 aatataacac cgtatccacc atatgttcta acattcttgg gccggcgatc gagccttctt
     8641 tggtaacatg accgacgata aaaatggcta tcccatttgt tttagcggtt ttgagtagtt
     8701 ctgcagtggt ttctcttact tggctgacac ttcctgccac acttgtgatg tctggctgcg
     8761 tcatcgtttg gatggaatca atgataacat aatcagggga gatatgttcg atggcacgac
     8821 tgatttcgtt catatctgtt tcagcgtata gataaaattc tgtgttgata cttccgaggc
     8881 gttccgcgcg taattttatc tgctctgcgc tttcttcgcc ggatacatat aagacttttc
     8941 cgcctatggc tgctaattgc tgagaaactt gtaacagcaa agtggatttc ccgatacctg
     9001 gatcgccacc aatcaagacc atagaccctg gcaccacacc tccgcctaac acacggttca
     9061 gttcttctaa ttttgttttg atccgaggtt cttttttagg gacgacgtct gcgatttttg
     9121 ttggttttgc tttttctcct gtcaaacttg tccgagttct gcggtccgtt gtatcttgtt
     9181 cgatttcttc gaccatcgta ttccattttc cgcaatttgg gcagcgtccc aagaacttag
     9241 gcgacacata cccgcaattt tggcaaacaa attgtacttt tgattttttt gccattgtac
     9301 cgcctccttt tatcctaatc atgagacatt aaatcaagct taagtatctt taacgtttta
     9361 tttactggaa gaaccaaatc cgccttgacg ttgagcagtc gtttgatctt gatctgcttt
     9421 taaaaacggc aaaaagatcc cttggccgat acgctcgcct tttttgatca ctacatcctt
     9481 gaaaccaaag ttggtgaatt ggaacatgat atggccttca tttgcctcat tattgtaata
     9541 atcactgtca atcacaccta ctccgtttgt caacactaaa aaccgcttca acggattgct
     9601 tgaacggtta gcaagctgca aaaattcatc ttctcccatg taagctttga ttccagtagg
     9661 cacgagggtt ggtttcaatt gtttttcatt ttctgataca tcttttttca atccatctgc
     9721 gactgtttcc aacccctttt tccagcctgc tttccagata cttggaatca cgacctcttc
     9781 tgctgcttca aaatcatagc cagcagcatg atgtgtagcg cgctgcggta gattgatatt
     9841 ttgatcttta tatttagaaa taacttcaaa tcctcttttt ctcaccggtt ttcctcccta
     9901 aataaactac tcctattcta acatatccga ggctgtaaaa gactttcttt acaaattctt
     9961 caagaaattc tgctatctta aagtaaacca ttaattcgtt ctttttccaa acgactgcaa
    10021 gttgtacaaa aaaactattt tttacatgac tttactcgtc aaaacaagta gaatagaacg
    10081 aaattaatga gtggatattt gattgaggtg tcacatgcgt aaagaaatta aaaatgcaaa
    10141 acgaattatt gtcaaagtag gaacaagtac attgatttat cccaatggaa atatcaatct
    10201 tgcggcaatc gatcagttag cttttgtcct ttctgatctt agaaatcggg gaaaggaagt
    10261 gatcttagtc tcatcaggag ctgtaggcgt tggtatgcat aaattacatt tgtcagaacg
    10321 tccgactttg attcctgaac agcaagctgc ggcggctgtt ggtcaagccg aactgatgaa
    10381 tatctataat caaagattca gtgtatataa tcaacagacg gcacagatcc tcgtgacaag
    10441 agacgtggta gattatcctg aaagtcgtaa aaatgtgagg aatacattgg aacagctttt
    10501 gattatggac acgatcccta tcgtgaacga aaacgatact gtagcagtgg atgaattaga
    10561 tcatctgaca aaatttggcg ataatgatca gctgtcagcc atcatggcaa aacttgtcgc
    10621 ggcagacctt ttgatcgtcc tttctgatat cgacgggttt tattcagata atccgactac
    10681 aaatcccgag gctcagatgt atcgctatat cgataaaatc gatgaaaaga ttctggcaca
    10741 agcaggtggt gctggtagca catttggaac aggcggtatg cagagtaaat taaaagccgc
    10801 ttctaggata tttgaagcaa acagcgcaat gattttagca aatggaaaaa atccggcaat
    10861 tatttttgat attcttgcag gaaaagaagt aggtacttta tttaaggagg acttgaaatg
    10921 actgacttga tgcaattagg acaacaggca aaagatagcg cgcaacaact agcacttatg
    10981 cctacaaacc gcaaaaatga gctacttgtt caaatggcac aaaccatcaa aaagaatcaa
    11041 caagcgatca ttgaagcaaa tcataaagac ttagaaaaag cagtcgaaaa caatatcagt
    11101 gaaacgatgc aagaccgctt gcagctgaca cctgagcgaa tcaatgccat ggctacagag
    11161 atcgaaaaaa ttgcttcgtt agacgatcct attggaaaag tagatgagat gtggacgaac
    11221 accgacggat tgagaatcgg caaaaagcgc gtgccactag gcgtgatcgg catcatttac
    11281 gaatctcgtc caaatgtcac gacagatgca gctagtctag cattcaaatc agggaatgcc
    11341 gttatccttc gcggtggcaa agaagcattc ttctcgaatc aattattagt ccaattattg
    11401 cagcaagtac tggtcagtgc tggcgaatct ccttatgcca tccagtttgt cgatgatact
    11461 tctcatgaaa ctgcaggaca gttgatgcga ttgacagaat acttagatgt gctgattcct
    11521 cgaggcggag caaaattaat ccaacgagtc aaagaacagg caacggtacc tatcattgaa
    11581 acaggtacag gaaataatca cgtatatatc gataaagaag cacagttatc tatggcagtg
    11641 aacatcatcg tcaatgcaaa agcttcccgt ccatctgtct gtaacgcggc agaaacattg
    11701 ctgatccatt ctgagatcgc accttttttc ttgccagcta ttgaaaaaga actggtagaa
    11761 cacggggtct ctttacgtgc ggatgctcgt gcgttagaat atctagagac ggctgcttta
    11821 gcagaagaca cagattggga tactgaatat ctagattata tccttgctgt caaagtggtc
    11881 gattctttgg atgaagcaat tgcacatatc aatcggcata atactaaaca ttcagaaacg
    11941 atcgtgacag atagttatgc ggctagtcaa cgtttcttaa atgaagtgga cgcggcggct
    12001 gtctatgtca atgcctccac tcgttttaca gacggatcgg tatttggttt tggtgcagag
    12061 attggtatct ctactcaaaa gttgcatgcc cgtggtccaa tggggttaaa tgaattaact
    12121 tccacaaaat atatcattta tggcgatgga caaatccgcg aataatgttt cgtgtgaaac
    12181 attattcccg ccttttgcct tttttctgta aaggttctat ccaactagaa aggtgtcaat
    12241 atgtttgaaa ttttaatgat tttttttatt taccttattt ctctgaatat cgctgctttt
    12301 ctgggagtcg gtattttatc ccttttcttt caattcaaaa aacgaagcat agggagtcaa
    12361 cgggaaaaat ggtcgcagta ttttgacaaa atcgggcaaa aagggctggt tacacgcttg
    12421 cacattagtt acatggttgc attatgctta ttagcgacaa tcaattacta ttcttttttc
    12481 gatcattcaa tcgcttatac gattacgcta ctgattgctg ggatttttca cttatcttat
    12541 aaatatcagc tgaataaaaa ccacttgaat cggactttcc gctaaaaaat atccgtacga
    12601 taagtagagt gccttgaaga tcggcaaaaa actgccggac tttttagagg ctgtgacaaa
    12661 gattttgtca cagcctcttt ttccatataa acggcgttcg cctaccagct gcctggtaaa
    12721 tctgtttcct cttcgttaag aagcgaaaat ccgtcaaatg tttcgccatc ataataacga
    12781 agcagctcat ccatggacaa gttcatcgtt ttttcttttt cttcttccgc tgattcagcc
    12841 agtagctgtt cccgatacac ctgatacata ccagctattt tttgctgcat cgcttttcta
    12901 gtttcacctt ccgcattgat tattggcaat tctttgatat agccacgata aatcaaccga
    12961 tcatctgaaa attcaatggg taattcaacg agtgtcaacc cgacttcctg aaaatattct
    13021 tccagcatct attatccccc cattcttttt cttaaatgaa ttttagctta cctactgaaa
    13081 attttccagt ttcattattt tgagaaaaaa agggaatgtg acaaaaatcg tgtcacatcc
    13141 cctttttccg aataaatggt gttcttctgc caactcctca atcataagtc acaatattca
    13201 acaaacatga gaaactgttt tttgaaattg ttccttgtga ttcagagtta aacgacagtc
    13261 tcacaacctc ttggtaaacg gcgttcaatc agctgaccct cggtattagc tcaaaaaacg
    13321 gtaaaatatg agaagctatt tttccgtttt gtttctctaa tacttaggat aaaacgctga
    13381 ttttacaacc tctgttaaac ggtgttcaat cagctgtcct tcggtattag ctcaaaaaac
    13441 ggcaaaatat gaggaactat ttttccgttt gtttctctaa tactcaggac aaaacgctga
    13501 ttttacaacc tcctgataaa cggtgttcaa aagcttgcat ctgcggtaat aagcccaacc
    13561 aaacgaaaaa tatgagaagc tatttttgtt tggctgttct tatgacttga tgcaggacag
    13621 ctttttcaca acctctgatt tcttattctt cttcatttgt agcaaaataa ttacggtgat
    13681 aaatgataca acatacactg ataagaacaa tggcacctgc aaaataaaac gcatagcttt
    13741 caccaatatg ttcaccaatc acacctgcaa taaatggtgc taaagctcca ccgaaaaagc
    13801 ggacaaaact atagctggac gaagcaacgt ttcgttgcag acctggtatt tccatagcaa
    13861 tcgtcgtcaa cagtgtattg ataattcctt ggaaaaatcc tgctaacaca atgctgatcg
    13921 gtacaaggac tggcatagaa gttccagctc ctaataacag caaacaaata atgaataaga
    13981 caaatgctgc taaaattgtc cgataagtcg taaacttccg ctcaagagcc ggtgcaacga
    14041 aaatcgaagc aatagccaga agcacacccc agccaaaaaa tacaaaaccg acttctaatt
    14101 cagaatagcc aatcaataaa aatggcgcat acgcaagtaa agtaaagaat ccgaaattat
    14161 ataacaatgc gattagtcca actgaacgaa gtttatgatt gcttaacgct ttgacacctt
    14221 cccaaaggcc tacttttctc tttggtttct cagtttcagg taaaagtacc gctagggcta
    14281 taaaaccgca aaacatcaaa aatgctaccc caaaaaatgg ataacgccat gaaccactgc
    14341 ccaagacccc accgactaga gggccaacag ccattccgca gccaagtgct gcttcataca
    14401 tcatgattgc tttttctgtg ttaccaacta aaatactgac gatcgttgct aaagcagtag
    14461 agacaaataa agcattcccg acaccccagc cggcacgtaa cccaatcagc attccgatat
    14521 tcccagataa tccgccaaga ccagcgaata caacgatgat aaacaatcct gtcatcaatg
    14581 tttttttcaa accgatccga ctagataaga aacctgtaaa tagcatcacc acaccagtga
    14641 ctaacatgta actagtaaat aacagggtcg tttcagctgg cgtagcgttt aactgtgcag
    14701 caatcgtttt taagattggg tctactagac ccactcccat aaaagcgatc atacaggtag
    14761 ctgctacaac tagcgctgaa cttttcccct ttgtttcttc atttgtttgt tcttgtatat
    14821 ctatttgatt ctccaatatt tccatccctt tctctctatt agcatcatac atgatttatt
    14881 ttacatgtca atatgcacat gtaaaacgtg gaaactttac tgtgatataa taaagaaaat
    14941 aagacgagag gtaggatttt tatgaatact ttagcctata tattattatg cgtgctttta
    15001 tccaaaccac gttctgggta tgaattgaag cagctgatca caattttttg ggaagcccat
    15061 cacagtcaga tctatacaac gcttgccaag ttagaccagc agggttatgt gaccgttttg
    15121 gacaaagacg agcactctca aaaaaagatc taccacttga ctgatgaagg acgtgctctt
    15181 gtagaggaat ggatcaaaga ggaaactccc gctcctacgc aaaaagatga atttctagca
    15241 aaaatctatg ctttttcgac gctcgataaa acaacagcta ctggactgct attcactaga
    15301 gaacaacagc tgaacaaaat catgcaaaaa aatcaaaaaa aattgaatga cctctcgcta
    15361 gacaagaaac aagaatttgg acgttatgtt gtgatccagc gcagaattat gctttgtcag
    15421 caggaactcc aatggtgtca gcaagtaaga gaacaaatgc agcagttttt tgagtaaggg
    15481 ataaacagaa cctttctaga aatatctatt gaagagcaaa ttatcttcaa taaaagagca
    15541 atacagttgg aaaagaaaaa gcgatccgaa cgacaggcca aaacctatcg ttcggatcac
    15601 tatttttcta gaactgcatt caagagacta tgacaaaaag catgccaagg ccttttttca
    15661 aataacagtg ttccatcatt cattactttg aattgctttt agcacacttc ttgaaagcgg
    15721 gccaacgatg atcaactgca acggtaaagc ggcaatcaca ttcattttcc aagcattcat
    15781 ataagcagag aataattgat caggtattcc ttgttccatg atcacaccga ataaagacat
    15841 acatgtcacc attcctaaaa ccatcaagca tgaaatagtt aagatcattt ggaccttgct
    15901 ttctggattt actggtagtt taaaggcaat ttttttcgcc agcacaccga tgatgaagac
    15961 atccaaaatg aaagccacca cgaatccagg cactagacca atcaacaaac ttgataaatg
    16021 aagatcttga tgcagccata aattatacgc gctcatcccc aagaccatca aaaaacacat
    16081 cattgttgta aaaattattc cttctttttt atttgtaggc aatctgtacc actcctttaa
    16141 atttgtcacg aaaatcatcg tagcacattt aaaaatttct cgtaagtact ttttttattt
    16201 tctgcccttt tatttcataa acttcatcag ccgtacgctc gacaaagtaa gaatcatgag
    16261 aagtaaacaa aatcgttccc ggatatattt gcattagctt ctctaaagct tcgatcgttg
    16321 ccatatcaat aaaattagtc ggttcatcta acagcaacac attacttggc ttagtaaaaa
    16381 gcaaagcaat cgtcagacgg gtcgcttctc ccccacttaa tgtacataac ggtttcgtca
    16441 aagtttcctg agcaaaccct aagtgattca gcaaactacg gacaagcgat tctgaaaact
    16501 ctgtttgtct catgacaaaa cgaatgatgg attcttcaga tgtcatttga taaccctttt
    16561 gctgatatac ttgaaagctt actttcggag agagaaggat tccttctcct tgtttgcgga
    16621 tatgttctaa taaggttgtc ttacctgaac cattttcacc gacaatcgcg atttttttac
    16681 ctaaagaaaa ctgaaaatca ccatttacca gtaatgttct cccacttctt tccaattgga
    16741 cattttgtcc catgatggga tagcggctgt ggagttcaag agattttggt actggaaaac
    16801 ggatttgttt catctgctgc ggcttggttg tttctcctat tttttggagt cttctctcta
    16861 atgttttcgc attcttttgg atggcttttt gtacactgtc tttttgttta gaggaagaca
    16921 accgatccag tctgatttgt tgctttttct ttttactgga cactttttcc aacttttccg
    16981 cttgtttttt cttttcttgg atcgattttt ccaatcgttt tttctctttc atgatcgact
    17041 ctgcttctcg tagctgagtc tttttctcca attccttttg agtgagatag gcactataat
    17101 tccccgaaaa ctctcggatt tctccgtctt ttacttccca gattttcgat gctagctcat
    17161 ctagaaaata gcgatcatga ctaacaaaaa ccagcgtacc ataataataa cgaagctctt
    17221 caatcagtcg atcaatactt tttctatcta gatgggttgt tggctcgtct agaagcatcc
    17281 cttgttcata agaagaaaga agacgtatca actcgatctt tcgttcttcc cctccgctcc
    17341 atttccgctc tgaagtttct tccagtctta actggaaatg actggctaat tccaaattta
    17401 ggtcctttgc ttcagcgaga taggttaatt gaggcaggta gttgaaggtg atctctgttt
    17461 ggattttccc atgatcggga aaaagttcac cggctattag tttgagcaaa gtcgatttac
    17521 cgacaccatt ttctcctatg atccctatac gatcttgctg atagacacga agagaatcaa
    17581 tagatagttc tagctggttg ccaaagctga cttgtagatt tgttatattt actgctaaat
    17641 tttccatatg ttttacactc ctatttcatg taaaacaaca attatcgata ctaaaaaaga
    17701 gcagaaaaat ctgtttgcaa aaaaactgca tcacatcttt tcctactctg aacgaaataa
    17761 ctatgactaa atcaccaaat ctgtcatagc tttcacctat tctactgtcg ttcgaaaaaa
    17821 tggacatact ccatccatga aattgttgtt tatacatgtc ttttgaattt atttagttgt
    17881 tattcaaaag ttcgaaacgt aatttcatcg atgcagtcat ttttccgacc tcctttttta
    17941 ttaggttacc acacacgatt caaaaaaagc aatagctact gaatagtaac tgaaaacctt
    18001 ttattcgaac atagtgatct tttctggtgc taaacgaaca gactcgtaac cttcttctac
    18061 cgcttttcct attgataaaa taataactgg aacataacgg tctttatcca aaccaaatgc
    18121 ctctgccaac tgatctgctt cgaatccacc aattggattt gtttcatagc catgatcacg
    18181 cgcaactaac atgaattgca tagccgctaa gctagaatcg actttgacta catcgttcat
    18241 ttcttctctt gaaaaactct tgtaataagg aatgatcgca gctaattgtt gatctctgac
    18301 ttcttttggc atcttccctg attcgtaagc ttggttgtag atttcttcgc catattcgta
    18361 acattgcata tcgccaaaaa tcatgaccat tgcagaagaa gtatcatttt gtctcgtatt
    18421 gaaacggatc aaaggacgca agatttcttt tgcttcctcg ctttcagcga ccacaaagcg
    18481 ccatggtttc atattgacag aagaaggtgc agtcgtcgct tctttgatca tttccagcat
    18541 ttcttcgtgt gggattttgt atgtttcatc atatacgcga acggatcttc taccagtcat
    18601 aattgttgaa aaatcatttt tttgttccat tattattaac ctctttcgtt tctgttgtta
    18661 tattgcaaag ttgggtattc aatttatcta aaagtgttag aaattgtttc atttctacat
    18721 cgctgaaagc cggggatagc acggaacctg tatctttatg gtccatttca caatgcttga
    18781 ttttcttctg ggctttttct gttagcgcta catacacttc ccggttgttc tgaggatttc
    18841 tctgacgcga aacatagcca tttgcttcca ataccttcag atgacgagta actgctcctt
    18901 gatcaatctg caacttttct tgcaaactgc tttgtagcaa tggattttct tctttcagtc
    18961 tcatcatgat ctcgtatcta gtaagactta taccgatatt ttcttcaaag atctggacga
    19021 ttttttgatc ggctaatttg atttgatata aaaggtgact aatctcttct aaagacataa
    19081 tttcctcctt tctaataatt gactcgtcaa cgattgactt gtcaataata gaataaaaaa
    19141 agaaaaaagt caaagaatca gctgtgtaat aaaaaaaacg ctctcaatag aaaagtatag
    19201 agaaaagaaa ttttttgtag aatgaacgga aaaaatgata aagaaagcac aacgctaaaa
    19261 ccaagattat tgattcttat caaactatag aagatagctc ttctgcagta gataatttta
    19321 caccgccaaa aatttccgaa atcgcctttt gcaagaatcc cgtcgaatat tagaaattgg
    19381 atcagtatgc ttaaacagta ataaaaaagt tatgacaaga tgttgtcaca accttttttg
    19441 agtaaacggt gttcaaaaac ttgcatctgc ggtaataagt tcaaccaaac aaaaaacatg
    19501 aaaatctgtt tttgtttggc tgttcttact acttgatgca gggcagcttt ttcactacct
    19561 ctttttaata accataaaat cctattcctt catatctcca gcccatcggt gttagccaat
    19621 ctctttcctt agcactcaag gtatagtgat gggtaccagt tttttcattt ggattaaata
    19681 gtcgataaat tggcttggaa ccaccagaat aaaaacttag accttcataa cgccaaccga
    19741 tttttgttaa agagttgatc tcagattgtg ccattgtgta atgatggtct ccactgtaag
    19801 gattgtataa acggtagaca ggttgtccgc tgtttggtgc ttgccaagca atcccttcat
    19861 attcccagcc tacagttggc agataatttt tttctccggc attcaatgta tataaatgtt
    19921 ctcctgtatg acggttatac agacgataca tcggagaagt tgaagttggt gctgtatcat
    19981 actgtgtcag attataggtt tcgattacat tgttcaatgt agaaccataa ttaggcgcag
    20041 tggcataacg acctgttagc caagccgtag catctttgta agagttcgta ttgcttttcc
    20101 atgcaccgga atagtagtaa acgcctggct gaaaagaagt cgtcttcaat accgttacgt
    20161 tatcttgcaa tgattctttg tacgaaggat attttctaaa agcggcattc accgtaatcc
    20221 attgcccatt cacatattcc gaagtaggca tcgtaacaga ttggccatta tagcttcctt
    20281 taatcccaaa tagattgtaa ttaggggcag aagccaaagt actgttcccc cagccacttt
    20341 caacgatggc ttgagcaatc atgactgatg catataagtc attggcactt gccaaacttt
    20401 gtgcagttga ggcaatacta tcaatgaacg cttgttgaga attagcagat gagcgagcat
    20461 aaaaagcata gccagtatag ttttcgaatt cagacaaatc tgcttgatgg gtatgtgtca
    20521 atgtttgccc atctgtcaat tggttcgtga tttcttctgc attctctgga ttgttctgag
    20581 cttctgctga tgtactgtct tcggtttgtg tttgttctgt tgtttcagtt gttggttctt
    20641 cggttgaaat tgtattttct gagacagtag ctgactgcaa agtactttct gtatcgtttt
    20701 caattcctgt ttctgaagaa ctcgatgtct gagttgcgac agtttctgtc gattcttcag
    20761 ctagcactgt tgtgttggca actgctagat ttcctaaaag aataacgcta aataataaat
    20821 tctttgcttt catctttttt ctcctttgaa ttatgaaact tttcctaaat aacagtgtaa
    20881 ctaaaaaagt aaaagcaagt caatgtccat atttaaataa catttgtttc tttttaataa
    20941 atttgctttt tctgcataaa aagacgtaaa tgaagaatat tcttctccat tcacgcctta
    21001 aattgatggt cgtttgaata actatttaac aagtaattct ctctggatat tatccgttat
    21061 ggattatact tcctgtgtcg tcggacggat aatcatgttg ctgacagaca tccggtctgg
    21121 cgtttctatc gcaaatgcta cagcagaagc tatatcttca gatgttaatc cccattcttt
    21181 ttgcgcttca tgaagttcaa gagcagcctt tttgtcgctg atggtttgat ataattcagt
    21241 ttgaacagct cctggagaga tgatcgtaga tttgatgtta ttttgccgtt gctcttgacg
    21301 taatccttcc atgattgcac ggacagcaaa ttttgttccg caataaacgg cggaatcagg
    21361 ataaacaaca tgtcccgcca cagaatcagt tgaaatgata tgcccagatt tttgtttttc
    21421 catgattgga agtacggcgg aaattccatt taacacaccc atgatgttga tatcaagcat
    21481 attttgccat tcatcacggt gaccttcaac taatggagcg gttggcatga ttcctgcatt
    21541 attgtacaat acatcgattc gaccgaactt ttccatcgct aaatcgataa ctgcttggac
    21601 ttgtgcaaaa tctcttacat ctgctgtttg aatatatagc tcagcttctg gtaaagattc
    21661 ttttattgct tttaggcgat cttctcggcg tgcagctata actagtttag cccctttttc
    21721 tgctaatagc ttcgttgttg cttcaccgat tccgcttgaa gcgcccatga tgacaatcac
    21781 tttttctgat aaatcattca ttctattctc ctcctgtttt gaaaaatctc ttgcttatac
    21841 tcactatctc ttatttcatc ttactgttca aataatccgt tcgacaggtt tgacgatttt
    21901 ggcagggatt cctgcaacga tggtattgtc tgggacatct ttcgtaacag ttgcattcgc
    21961 tgcaacaatg gcattctcgc caatcgttac gcctggcaga atagttgctc ctgctccgat
    22021 ccaagcattt ttcttgatgt ggattggctt tactgacaag cctcttcgtt cttttggatc
    22081 ggttagatgg ttcaccgtga tgaggttgac acgaggacca atcaatactg aatcttctaa
    22141 acagatgcca cctaagtcaa cgaacatcac ttgacgattg ataaagacat ttttccctat
    22201 actaatatgt ttgccgaaat ctgtataaaa aggcagcaga acagtcaccg tatcatctac
    22261 tttgtcacta atgatatttt ctagccgttt gcggatatct gctggttcat ggtacccagt
    22321 attcagcgct gctaaaggct cttcgttttc cgcttggatc tgatggatca cttcaaagag
    22381 cggatccgtt actcgaatct ctttattgac gatttgtttt tcgatgaggc ttgtcatttt
    22441 gctccctcct tttatcctta ctttataacg gacggcttat gatgtctaat acttataaaa
    22501 catacttaac tattcctttt aactatactt agtggtatgc ttttttaaaa acaggaggga
    22561 aaaaatggat attcgtatac tgacgtattt tttagcagta gctagagaaa aaaatatcag
    22621 ccgagcagca aaatctctgc atatcacgca acctactctc tcaaaacaac tgaaagattt
    22681 agaagaagaa cttggtgtga cacttttcac tcgaggctcc cgagagatac agttgacgga
    22741 ttctggtcga tatctttatt caaaaggaaa agaaatcttg tcgttggtag aaaaaacggc
    22801 gaacaattta acaaatgacg caatcatctc tggagaaatc tacattggcg gaggcgaaac
    22861 taaagcaatc tctttcattt ctgatagcct tcattcgttg atcaaggcgc atcctgatat
    22921 ccagtttcat ttatatagcg ggaatgcaga tgatatcata gacaaactgg atagcggatt
    22981 acttgatttt ggactcgtaa tcgaaccgac aaacaaacaa aaataccagt catttcgact
    23041 gccagcccaa gatcgttggg gacttttgat acatcggaat catcctttag caaagcaaac
    23101 agtcatccat ccgcaggaac tgaaaactgt tcctttgatc atttcccggc aaacgtcagt
    23161 ggacagtcaa ctagctgaat ggctaggttt cagcatagat cagttatcga tcgtcggaac
    23221 atataactta ttgtacaatg cttctttgat ggtcgataac gggacttttg gtgcattagc
    23281 tctagatggg atcatcaata cttatgggac ggaaaaagta ttcatcccac tcgatccgcc
    23341 attacaagct tcactgaatc tcatttggaa aaaagaccag cctctttctc ctgctgctcg
    23401 taaattttta gatgtcctaa cggagaaata cagttaagta gagttttttt ccttcgcttt
    23461 cagcttttta acaacgaata agttaagcaa aagatccaat gattaaataa cataagtttc
    23521 ttaatttgtt ccttcctttt atttctgata attccagtaa attactataa ttataaaaaa
    23581 agaattgaca aaacataact gtctatataa aattgattac gttaacaata attcgtttca
    23641 tgtgactaga tcatactagg catgaaaaat ctctgaggaa catggtttcc ctaggaggtt
    23701 tttcgtgtct atttttgttt ggccgcaatc aactggtcta gcaaacgtga aaaccaaatc
    23761 ttcaaaaagg gtgcgcgcat gaaaatcaaa aagatcttta atcaaaatgc agtattggta
    23821 gatgacggtg gacaggaaaa agtagctatt ggaaaaggga ttggatttga cagaaaaaga
    23881 aatgatttga tttttgatcg tgatgtcgaa cggatatttg tcatggaacc agaaggtcaa
    23941 atcaagttgc aaaatttact taatcagatt gatgaacgct ttttatttgc agcagaaacg
    24001 atcattgatc atgcggaaac tgttttgatg gaaaagttga atgaacatgt attgatcgct
    24061 ttgaccgacc acattgcttt ttctgcggag aacattaata acggtatcgt catccgcaac
    24121 aaacttttaa gagaaatcga agtgctttac agtgatgaat tcagtattgc tcaatgggca
    24181 gtagagtatc tgacaaaaga gcttgggatt ccatatactt atgacgaagc aggatatatc
    24241 gcaatccata ttcacagcgg acgaagcggt cggaacaata accatcgcag tatccgtgaa
    24301 gtgacgattg tatctgacat catccattta gtggaatcag aattggaaac agatatccat
    24361 tcagaaccat tttcactgaa ttattcaaga ctggtgaatc atttacgact gcttctacaa
    24421 cgaacccatg cgcaacaata tgctgtttta gacacggaaa tcgtagaaat ggtcaaacga
    24481 aaatacccag aaagctataa gatttctaaa aaaatccgtg tgttgctgac caaagaatac
    24541 cagctcgcga tcacgaaaga agaactgggt tacttggcca tccatatcga gcgtttaaga
    24601 agcgcaatcg tacaaacaaa tgttaacaat catgaggagg aaaaaaattg aaagcatatc
    24661 tacaacgaat gggacgttcc cttcaacttc cagttgcggt cttacctgct gctgctttac
    24721 tagtagggat cggaaactgg tgggcatctt atagtaacga cattgtcgct cattttttac
    24781 aagctggcgg taacgcagtt ttaggtcaat tacctttgct gtttgccgtt gggttagcac
    24841 tagggatgtc agtggacaaa tcaggtgctg ctgccttggc aggtttggtc gcatttgaag
    24901 ttccagtaaa tgttttaaaa actgattcag tagctacttt attgaatatc aaaccagaat
    24961 cagttgatcc atcttttgct cagatcagta acgtatttat tggtattatt gccggattga
    25021 ttgcggcagc tctttataat cgtttccacg aagcaaaatt accgatggct ttatctttct
    25081 ttagcggcaa gcgtctagtg ccaatcttgg ctgcctttgt catgttgctg atcagtgcag
    25141 tgctactgct cgtatggcct ccggtttact ctgctttagt ttcttttgct aaattcattt
    25201 tgagtttagg ctggggtggt gcaggtcttt atggcttctt caaccgttta ctgatcccta
    25261 caggattgca tcaagcaatg aactccgtgt tctggtttga cgtagcagga atcaatgata
    25321 ttggtaaatt tttggcttct gaaggaacaa aaggcgtaac aggaatgtat caagctggat
    25381 ttttcccaat catgatgttc gggcttccag caggagcata tgctatttac agaaatgcac
    25441 gtccagaaaa caaagcaaaa acagcttcct tgatgttagc agcagctttt gcttcattct
    25501 ttactggtgt aacagaacca cttgaattct catttatgtt cgttgcttgg ccattatatg
    25561 tcttgcatgc aattttcact gggatttctt tagcagtgag tgcattcttc cattggacag
    25621 caggatttgc ctttagtgcc ggatttgtcg attacttcct atctttgaaa aatccgattg
    25681 cgaatcagcc attgatgttg atcgtccaag gattagtaac agcagtgatt tactactttg
    25741 gctttaactt tgctatcaaa aaattccatc tgatgactcc tggccgcgag gaagcagatc
    25801 tttcagatga cgatactgca acgactaatt cagacaataa atatgcggca caagcaagca
    25861 aaatctacgc tgcattaggc ggagccgaca atgtaacttc gatcgataac tgtactacac
    25921 gccttcgttt gcaagtcaaa gatacaggcc tgatcgatca aaataaaatc aaagccactg
    25981 gcgtacctgg tatgaagatc atcgacggaa aaaatgcgca agtcatcgta ggtacagaag
    26041 tacaattcgt tgcagatgag atggcaaaac tacatggtgg tgctgcaaca cgacctgcac
    26101 aaacaaacac cgtggttaaa acagaaacat ttgccggtgg aacgatcgaa caagatattt
    26161 acgctatcgc aaatgggaaa ctgattccaa tcactgaagt ttcagatgat gtattcgcgg
    26221 aaaaaatgat gggtgacggc tatgctgtat taccagaaaa tggagaaatc ttctctccaa
    26281 tcgctggtac gatcacaaac attttcccaa cgaaacatgc tgtcggaatc caaacagatg
    26341 ctggtatcga agtcttgctt catatgggca tcaacactgt tgacttaaaa ggcgaaccat
    26401 tcactttata tgtaaaagaa ggtcaaaaag tcgcaagagg tcaattgatt gctttagttg
    26461 atcttgccgc tattcaatct gcaggcaaaa acacggatat ggtcgttgta ttcacaaatg
    26521 gcgacaaagt ccaaagctta gaaatcgaac ccgcacgtga tgtaaaagca aatgataaaa
    26581 tcggctctgt ttccaacaaa gcctgagcca tcgtcttccc cagacttttt tatcgataaa
    26641 caaacaggag ctgagacaaa aatcttgtct cagctccttt ttccgaataa aaacggcgtt
    26701 ctcctgccaa ctccgcggta ataagtccag ccaaacgtaa catatgaaga gctatttttt
    26761 atttgactct tcttattact tgatgcagaa cagctttttt acaacctctg ataaacggtg
    26821 ttcaaaaacc agtttatcgg tatgaagtca aatttatgca aaaatgtgga aagccatttt
    26881 cacaaaattt ttcttcatac tcaaaactaa tcggcttttt cacaacctct ttatacatta
    26941 ttcctgatcc aatatttctt ttaatgctgc ttcaagagaa gttaatggtt ttcccaatac
    27001 tttttctaaa tcatcagagg aaatagctaa ctgatccttt ttaatatcat gttggatcgc
    27061 taaaaacatg tcagctgctt cttgagggaa tccggcttct tttagatttt cgacaaacgc
    27121 tgcatcactt gcttcaataa tttctaactt tttacctgtc gcacgctcta atgccttggc
    27181 taattcttca tagtcagtta gattgccgga taattctaag atttctggag agtcactttc
    27241 tactagtact tttgctgcag cttctgcata ttcacgtttc aatgcccaac ctgtttttcc
    27301 atttcctgca gcataaacga attgaccagt ttttaaagcc tgcccaatga taggtaattc
    27361 attctctaca taccaattat tccgtaaaaa tgtatgggaa atacctgaat ccttaatcat
    27421 tttttcagtg aattggtgat ctggcgctaa tacactgatc gagtgatcgg catctgcaaa
    27481 acttgtataa gcgatataag ataccccagc tgcttgtgct gccttcacta cattcttatg
    27541 ttcggcttga cgatttcctg gaacgccaga gacaaaaagc aatcgatcta ttcctttcaa
    27601 cgcttctttc attgagtcta aatcatcata atcaccaata cgtatattaa atccagcttc
    27661 tttcagtgct tctccttttt cttcactacg tactagtaca tagatttcat tttgtggaac
    27721 catctttttt aagaattcca gcgcataacc accaaaacct ccagttgctc ctgttactaa
    27781 ataatgcata tttgccactc cctattcttt ttatttgtta agatttacag ctgtacattt
    27841 ataaattaca cctttatcat aaagagtgtg gtaagataag tcaagcaaaa aaaatgaaag
    27901 gatgacaact gatgaagtat tctctgcaat ttagtgatgc aatccatgta cttgcctata
    27961 ttgaaatgtt tcaagatacc cgatcacttt ctagtgagat gattgccagt agtatcgaaa
    28021 caaatgcagc aaatgtacgt aaaatcatga gtcagctcaa aaaatccggt ttgatcttta
    28081 caaaaaccgg aaaaccgcag ccttcccttg ccaaaagtcc agatcagatt tctttactag
    28141 atgtctatcg cagcatcgaa ggtaatacac agcttatcca agtcgatcca aaaacaaatc
    28201 caaattgcgt cgttggtgcg aatatccaag aagctttaga agaaagctac gctcggctgc
    28261 agaaaaaggc agaagaagaa atggctaaga tcacacttga tagtctgata cataaaatag
    28321 ccgttttgga aaaagaaaaa agaccagaaa acacaggcct cgtagagaga tttttataat
    28381 tctctctact gtgcctgcat ttttctgatc ggaatcgtct acataaattc ttttattctc
    28441 cgggggacaa aagccttgtc atgccccttt ttcctctcta atgctcaggt caaagcgctg
    28501 aagtcatgac gtcttgataa acggtgttca agtagctgac cctcggcatt agttctgaaa
    28561 aatgcaaaat atggaaagct atttcccatt tttcctctct aatgctcagg tcaaagcgct
    28621 actttcacga cctctatatt atcatggttt attgactacc ggtatccaga tttctcccat
    28681 atatttcccg ttggtaactc ccatttcgac tacagcattt ggacccccaa cataggcgta
    28741 atcttctact tctcttaaaa catgcccaaa ggccgtattc gtcaaatcat ttcttaattc
    28801 ttcaggcgat tctgctaaag cgattacaac aagatacgtt ccttttggga atgggatgat
    28861 tcgggtagtg tttggaacat cctgactcgt ttgaacaccg acatagtaca tcattttccc
    28921 gttatagggt tcgttgactg taaagaaata atgattttca gcagcttgtt tcaatgcatc
    28981 tattgttcca ttaccgataa ctttttgctc aaatgcttgt ttctctgcag ccaaaccgac
    29041 aaaatccgta ttgctactct tcaattccac accaataccc gtcaccgtaa aatctggttt
    29101 ttcttcgatc ctatactttg ccatttctcc caccctttct attatattaa gttcatctta
    29161 cataaaatta aagggaaatg acacgattac gctctttttt ctagggatat ttttattttt
    29221 taactatttt tttgctttca ctgcaatcca aatctcactg taaacattac tgaaatcctc
    29281 tccccatttt gtaaaagaga tctcgggagc ttcgactaac tcataatctg aagaaggaag
    29341 ccattctgca tagattttcc cccaagtatc ttgtaaagtt tggggaaatg gccctttatt
    29401 aggaaaaatg gcccaagtgt gttcttctat agcaagcatt tctaaatcag cgtacggatt
    29461 ttcttttgtc gtagcaaagc caatcatgtg catcaaactg cccttttctt ccaagcgggc
    29521 tccatcaaat ccataagaaa cgttgattac ttgatttgga tataaatctc ctaacttatg
    29581 catctcttcc ctttgagtct ccgtgatcgt ctgcgctaac ttttgtattt cttgattgac
    29641 tccttcaaat ttgacaggaa cttttttcgc cacacctgcc acataaaact gttctttctt
    29701 ttcgatacga aattccatcg attttcctcc tctgacatca ataaaaaatg tcaagcgtgg
    29761 aaaagatttt tgtatgccgt atctgctgat ttctgaaggc agatatcctg accaatcacg
    29821 aaaagctctt gaaaaacctt cgactgtttc atatccatat ctaaacgccg tttctgtcac
    29881 tcttgatcca tctaggagat cgcgattagc caaagccaat ctacggtttt tgatatagtc
    29941 cattagcgac atccctgcaa taaacgtaaa gatgcgtttt aattgatatt cagagattcc
    30001 tgtattttcg gctattattt tataggaaag tccttctgtc aaatgttctt cgatgtaatc
    30061 gatcaattgg ttcagatctt taagcatatt tatcttcctc cttgcttgcc tttttatcat
    30121 attatcaaag aaagtcaatt acttgatgaa tcaggaacca atttagtcag atgtagaaaa
    30181 tgttgtatgg atctaaagaa aattctcaag cagcgtttcg taacggataa ttggtattat
    30241 ctgcctatct atgttttacc tttagaaaaa taaattttgg ggaagacaca ttctaagtaa
    30301 acaaagcggt aaaaatatct tttattccct tagttaatcc gcttactacc tactgtacaa
    30361 agcaaattgt tgttcaaact gctgatcaga acgctgcttg gatttttctt tgcgatacat
    30421 catacctctt ttatcaattg gaacaattat ttatttctaa caaaatcact tcatttttgt
    30481 cagataaata aaaaacaaaa aaagttccat ctttaaaaca cgctattctt ttactttttc
    30541 tcacattctc taagaaaaga aaaggagaat gaatttttga caattcgagc aaaagtgatt
    30601 atcaaatatg taaaatcagc tacttttttc ttaaacgttt ctttatacta aatataaata
    30661 agtgtttcat gaaaggagaa ctagatgaat aattggcttg agtattttcc tgaaaacgtt
    30721 ttggaacgcg gttacagcta tcatctgcac ggttttgtcc gtcatttgaa ctatacaagc
    30781 aagtacttgt ccgcaacagt ttctggcacg gaagattata aggtagtgat cacttgggat
    30841 gaaaagacca acatgacttg tgattgtcta tatgccatag aaggaaaaaa atgcaaacac
    30901 atggctgctg tcttattcgc ttacgaagaa cgtccaataa aaaaatccaa ctactcactt
    30961 tccgaactct cgtcccttgt ttcctccgct agttcctctc ttgtacggga gcttttaaca
    31021 gagattctta tagaacatcc gcaatttatc gaacgcttca aagttaagat gccatttcat
    31081 gcaataaatt attcagataa attaacaact atcatccata aatacgatca tattatcaag
    31141 aaaaacaaaa acaggaaaac agcgaagttc atcatggaga tgcgaaagtt catccaagaa
    31201 gcagttgaat cgctcattca gcaaaatgcc tatcttcccg cattcgaatt gatcaatgaa
    31261 gtgattgcta ctctcgaaac attctattgg gagcctgagg atgaacgaac ccttcttctg
    31321 atagaggact gttattattt atggaaagaa ttattagcag aagcacctca tgcagaaaaa
    31381 aggcaaatgt tctcttggtt cgtctgccaa gtcgatcata ctgacgcctc ttattcaaaa
    31441 cgctactcca taaaaatcct gaaagaagac tttcgggaaa aagaattttc taaccaaaag
    31501 aaaaaaatag ataaaaaaac taaagaaacg gtaaagaaag acgatttcaa tgaaaaatag
    31561 caagtaaacg ataacaacca tcctaaatcc acaagaaata cagcagacct tgctttaaaa
    31621 acacgaatca tgcaaaggat gaagtaaaga agacttaacg cgtacccaat attagtcatt
    31681 tgactattag gtacgcgttt tgttttataa gtcatgacaa atttccttcg ttgctgggta
    31741 gattttccgg cattgttgat agattttatg atacttttct acattttgta agagaggttc
    31801 cactgcatct gtatattgga taaaattctg acaacaactt tcaggtgtgt cgaaccactc
    31861 taatctttaa agataaatat taaaataacg attaataata ttaacagtct ttatttttca
    31921 ccaccaaaaa cctatctttg catatactaa atagacgtat atacacagat aggtttgtct
    31981 tgtgaaaaat aatgattatc cttaccaaaa aatgtttgtt tttctacatg ttcgtactta
    32041 tcaaaaaata tgatccttat tgaaattact ggctatttat attccaacga cttcttgtgt
    32101 taatttttgt tatctcatag taaattatgt ttcattttca cttaatttag ttgtcacgtg
    32161 ttcgtcaatt cgtaaaaacg gtaaatatat aaacacactt gcggctataa ttaatatttg
    32221 taaaacagct gctcgccagt ctccggctgt tgctaaaaat cccgatataa ctggtggcgt
    32281 agtccaagga atttgcacta ctacatgatt aattaatccc acagcagttg caaaatatgc
    32341 tactgtcaaa gaaaaaatcg gtgcaagcac aaagggaata atcagactga tattaaatac
    32401 aataggcaat ccaaaaataa taggttcgct tatattgaat atagcgggag ttaccgataa
    32461 tttagcaaag tccttataat cttttctacg actgaagata aatattgcaa tcaataaagc
    32521 aatcgtattt cccgatccac ccattaccgc aaaggtatct ttaaaggcca tattgataat
    32581 atgtggaatt tgctctttgt ttgcataagc tgccatattt tcttgcatat tctgaatcaa
    32641 aaacggatct aacaatgaac ctgatattac tgcttgatgg ataccaaatc caaaaaatag
    32701 attagcaata gatgtaatta acaaaaatcc tggtaaacca gttgtaattt tacttaatgg
    32761 ctctgtaact atcgtggtaa ttagcttatt tatatccata ttaaacaatt gaatgattat
    32821 aaatgacaat aaagcaaacg aggtaatcgt aataatgatt gggattaata cattaaaaga
    32881 ctttaaaaca gcgggaggaa tatttccgga tagctgaatc tgtagtttct tattattaga
    32941 aagtttaata aataattctg tagccgctaa tccaacaaat atacctacaa acattcctga
    33001 agctcctgta taggatatag gtatcacacc cgtcacttca acagctttat ccgcattttc
    33061 aggagtaaac atcatctgca taggtgttaa tacaacaaat gtcgaaatag caactaatac
    33121 cgctgcaata tgatttgtat aatttctgct tgcacacata tgataagata ctgctgctac
    33181 aactaataat gtagttatgc ttaatgtacc attggcaatt gaagtaccaa tttgttgtaa
    33241 cgtggtcaat gtactagtat cgacaatttc ccccatgaat cctgttggtt ctaagatcac
    33301 tgaattaata agtgtcacaa atcctgctaa catcataaat ggcataaatg tagcaaacgc
    33361 atctcgcaaa gttcttaaat ggatctgatt ccccaactta gtcgcaacta taccaaattt
    33421 ttcaaataca atgtccttcc tgctactttt gcccatattt tttctccttt atgcttattg
    33481 taatcgatta ctttttccac ccaaataata catcacttat caaaaaaagt aaatactttt
    33541 aattaaatat atatatcttt tttagtataa aaaaataagc tctgaaaaaa taattttccc
    33601 gagcttattt ttcaacaaaa acagcttcaa aaacaaagtc ttgatataaa tgtctcgata
    33661 ccgaatactc gaaaggttta ccagacgata gagcaacaac tttgtctatc tcaatcataa
    33721 attcacctgg atctattttt aatatttttg cgtcaaattc attcgcagta tctcctttga
    33781 tacgtatgtg cgaagtcttt atttccagac ccagttcatt tgtgatataa gcatatacag
    33841 atttctttaa atgttttggt tccaagcctg gtatgactgc taatggcatg aatgtttgct
    33901 caatcgagta tggttgacaa tcaataaaac ggcttcgtac aatattatat acatagtctt
    33961 ttacactgat acttaattta tcagctactt ttttcgatgg aagttcaatt gagaaagtat
    34021 ggatttctga atctacgtgt tcttcaccaa ccacgctaaa caaaccttcg tttggaccaa
    34081 gaactcgtga gttatcaatt ggtgttctga cgacttcagt cccataccca cttctacttc
    34141 gcaaaactcc ttcggaaaca agtaagtcta cgcctttctt tactgttaac ttgcttgtat
    34201 gataaataac tgccaattca tctcctgtag gaagtttctc accaatttta tactctccat
    34261 ttacaatttt tttctttata tctaagtaaa tttccatata cttcgcaatt tttttcaatg
    34321 gtgaccctcc aatcaaaaac atatcttcat ctatacttta acataaataa accgataaaa
    34381 tccacatcaa aattatacac acaataaaaa agatatatat atttaaacaa aactatttac
    34441 tttaagttgt aagcggtgtt atcattaagt ggaaacagga ggtaaaaaaa ttatgacaac
    34501 tatgaaaaag ctaacttttc cagatgattt ttggtggggt tccgcttggt cagctgagca
    34561 agcagaagga aaaggagaaa caggaaaagc agagactatc tgggatcgct ggtttaaaga
    34621 acaaccttac cggttttatc aacgaatcga ttcctctatt acaaccgatc acatccatca
    34681 atataaagaa gatatagaac taatgaaaaa aactgggcat aactcctttc gcgtttctat
    34741 ttcttgggca cgaatgtttc caaatggcgg taccggaaaa gtaaatccca aagccatttc
    34801 tttctatcga aaattatttt ccgaaatgaa taataacgga atcaaagtat ttgccaactt
    34861 atatcatttt gacatgcctg ctactcttca agataaagga ggatgggaat cacgtgaagt
    34921 agttgatgct tatgttcatt acgctgaaac ttgttttaaa gaatttggtg acttagtttg
    34981 tcattggttt accttcaatg aaccacttgg gcctatttta ggtacttatc ttgaagattt
    35041 tcattatcca aacctcattg actttaaacg cggagcgcaa gcagcattct tcactatact
    35101 tgcacacgca aaagcaatac aagtatttaa agaactagct cttcctagta aaataggcgt
    35161 tattctaaat ttgagtccta cttttcctcg tagtcaaaat cctgctgatg tgaaagcagc
    35221 agaaatcgct gatttatttt atacgcggag ctttttagat cctatggtta aagggaattt
    35281 ttctaaaaag cttatcctgt tattacaaga atataatcag cttcctgaag attatacgaa
    35341 agaagatctt gtatgtatct acgaaaatac cgctcaaatt cttggactaa actattacga
    35401 gccacgtcgc gtcaaatctc gttcttctat ggtggataca tctagtccat ttttaccaga
    35461 gtggtttttc gaaccacata tcatgcctgg acgacgaatg aacgaatacc ggggatggga
    35521 aatttacgaa aaaggaattt atgatttgtg catggatatc aagaaccatt atggaaacat
    35581 cgaagcattt atctcggaga acggaatggg cgttgcggat gaagaacgtt tcatggatga
    35641 tacagggcaa ataattgatg attatcggat tgagtatatc aaagatcatc ttgcttactt
    35701 atggaaggca ataaatgaag gatgtaacat taaggggtat catctttgga catttattga
    35761 ttgctggtca tggatcaacg cctataaaaa ccgatatggg ctggtttcac tcgaccttac
    35821 tactcaaaaa agaacaatta aaaaaagtgg cgaattttac aagcaattaa gtgatacaaa
    35881 tggatttaga tacgatgtag acttgttagt tgaataatat tctaaaattc cacttgaagt
    35941 gaacacatta gaaaacaaga ataatgagaa ataccaataa caaatacttt ttcttcatac
    36001 ttcatttata cttgtcagta taagagactg tgacaaatgt agtgatcctc agaagttagg
    36061 tctggaaatc taacttgaga agattttttt aaactacgtc cagtcttttt tatttgttta
    36121 aaattgaagt tgacaagtct tcattaaaaa tttagaatat tcacaagttt gacgacaatt
    36181 atgaaaattt atgtcgtgct aaaggagttt tgttattgga tgaaaagtta gatataaaaa
    36241 ctgcgatcaa agacacgtta cctaccgttt ttggttatat cggtattgga cttgcatttg
    36301 gtatcgttgg gaaagctgcc gggtttcatc cgctggtcgt cacgttgatg tccctgctga
    36361 tctatgctgg ttctgcccaa tttatcacgg tcagcatgct tgctagccat agtccactgc
    36421 tctccatcgt tctctcgacc tttctagtca attcccgaat gattctgatg agtatgacga
    36481 ttgctcctta tttcaagaaa aatcggctgc ttcaaaatct gctgattggc acattgctga
    36541 cagatgaaag tttcgcactg ggaatgaata aattgaacta tactggacaa aagttaaact
    36601 ttcgttggat gaatacagct aattggattt cttatttgac gtgggtgggt tcttctcttg
    36661 ttggcgcact tttagggaat tttattactg atccgaaaaa gtttggtttg gatttcgcca
    36721 tcgtcgctat gtttatcggg ttactttatc ttcaagtcat ttctgataga aacacctcta
    36781 aacgattaca gttgatcctg attggtctca ctttgattct ggtatatgtc ggactgattt
    36841 ttatcccaag taacttagtg attttagttg taacactgat tgggtgcgga ttgggggtgt
    36901 ggatcaaaca tgccttcttt tgagtttaca ttgctgacta ttttaggttg tactttagca
    36961 acttggcttt ctaggatcct accatttgtc ttactgaaaa aattcgattt gccgcaaccg
    37021 ttgcttgagt atttaagttt tgtcccgatc gtcattatgt ctgctttatg gttcagcagt
    37081 ctatttaccc aaaatatcgg acatctgccg cagatcaatc tcgaaaatgc tttggcttca
    37141 gttcctacac ttttagctgc tattctttca aaaagcttat tggtgatcgt cttggtagga
    37201 attctttccc tttcgttgat tcgcttatta taaataaaaa aacaagagct tatggcaaat
    37261 tactgtcatg cgctcttgtt tttatcaaag tcaaataaga aaaaaatcgg cgtattcttg
    37321 cttatccgtt gaacggacca ctaacaacga cttttcctaa tatatgtcct gtttctacgt
    37381 ctttggttgc ttgtttcaag ttcttcgcat tcatcccatc tgaatagacc ttgctcaatg
    37441 tactgcggat aatcccttca tttgctaaag cagcaatgtg cgctaaagct tctccttgcg
    37501 tttcgatttc atagttatag tctgttttag cgaacatata ctcccagtcg aagctgatag
    37561 attggttttt ccaaacacct atatctaacg gctctttgat ccctacgatc gttcccacat
    37621 gtccaaatgg tcggacaagg tgtttgattt gatcaaagta gcgggtaata tcgaataata
    37681 cagcgacata ctctacttga tcgattccta attcacgaaa acttttactt aagtcgtgat
    37741 gataatcgat tggatagtct acacctgttt ttttcagcca ttcaaaattc ttcggacttg
    37801 cagtcgcgta gacttctagg ccagcccaat gcgctagttg attcaaaatc gaaccgacgc
    37861 ccccagctcc gttgatcact aagatttttt tcccgcggtt ggcattttct tctggagtca
    37921 gaccaaattt ttcaaataac aattcataag cggtcaatga ggtcaacggc aaggctgcgg
    37981 cttcttcatc tgaaagattt tttggtgcaa gagcgacaat acgttcgtca accagttggt
    38041 attcttgatt gcttcctgct cttgtcgttg tacctgcgta aaaaacgcga tcgccgacag
    38101 aaaatttttg cacttcatca cctacagcaa caactttccc gactccatca aaccctaaga
    38161 tacgtaaagc atttcttatc ccgttatctt ggcgaagttt cgtatcgaca ggattaacag
    38221 atactgcgtt gacttttacc agaagatccc gtgaactggg tacaggtatc tgttcttttt
    38281 catcaataaa gctttttgga tcatcgatcg acaacccttc ataaaaaccg actaccttca
    38341 ttaattcctt ttctgacata cgaattcctc cttagcttca tcaagttgga gcatccatac
    38401 agttttcatt tcaatatata tagtatgcta tttgttccac ttttattcat cataattatt
    38461 ctcttactta aagtaaacta tttgcacttt tttggttaaa aaagaggttg cgacaagact
    38521 tttgtcacaa cctcttttaa acggtgttca atcaactgac cctcggtatt cgctcaaaaa
    38581 acgacaaaat atgaggagct atttttgttt ggctgttctt attacttgat gcaggacagc
    38641 tttttcacga cctctaattc acggtgttca agcaagaaat cattgttcac tgaaaagcgt
    38701 tggatgttct ttttcaaagg tgtctaagat ctctcgtact tcatcagtag atttcgtatt
    38761 catcaactga actcgcaagt catttgctcc tggaaatcct ttgatataga ttttaaagaa
    38821 acgatgtagt cctgtgatgg aacgcgggat gatttctgaa taatgatctt gcagatctag
    38881 ttgcagacga agtagtccga tcagttcttt ttctgtatgt tccctcggtt ctttttcaaa
    38941 tgcataagga ttcttgaaaa tcccccggcc aatcatgatg ccatctacgc catatttttc
    39001 ggccaattct tctcctgtct ggcgatctgg gatatctccg ttgatcgtga tcagtgtttg
    39061 cggagcgatt tggtcacgta aagcaatgat tttagggatg acttcccaat gtgcatctac
    39121 tttactcatc tctttacgcg tacgcaaatg gacagaaaga ttggctatat cttgatgaag
    39181 taggtgactg atccattctt ccatctcaga catttccgta tagccgatac gcgtcttgac
    39241 gctgacaggc aaaccgccag ctttagccgc ttcaatcaat tgtgccgcta cttctgggcg
    39301 aaggatcaag ccactgcctt ttccacgttc tgctacatta gggactgggc agcccatgtt
    39361 gatatcgatt ccttggaagc ccatttctgc catagcaatg ctcatttctc taaaaaattc
    39421 tggcttgtct ccccaaatat gggcaaccat cggctgttca tcttctgtaa aggtcaatct
    39481 tcctcgtaca ctgtcttttc catctggatg acagaaactg tctgagtttg taaactcagt
    39541 aaagaagaca tccggtgctg ctgcatgctt tacgacatgg cgaaatacaa catctgttac
    39601 atcttccatt ggtgctaaaa caaaaaaagg ctttggcagc tctttccaaa aattagtagt
    39661 catttatttg ttactctcca ttctatcaag cagtgaagtc gcaatctttc tcaaaaacat
    39721 tgtcattata ctaggaaaca aaaaaagaag caaagtgaac agcttaaaag atgtttctga
    39781 ttgagcaaaa aaagactgtg acaaaaatct tatcacaatt ccttttccgg aataaatggt
    39841 gttcaaaagc ttgcatctgc ggtaataagt ccaaccaaac gaaaaatagg aggagctatt
    39901 tttgtttggc tgttcttatt acttgatgca gaacagcttt ttcacaacct ctccactaaa
    39961 cggtgttcaa tcagctgacc ctcggtatta gctcaacaaa cgacaaaata tgaggaacta
    40021 ttttcccgtt tgtttctcta atactcaggt caaaacgctg atttcacaac ctcttttcta
    40081 acttctcaat cctcttcctt tattgatcaa ataccaagta acggcgtaca aaacaacgat
    40141 gaatagcaca agtacgatca tcgatgttgc tactggcaca tcgattgttc caaggaatcc
    40201 gtaacggaaa ccgaagatca tataaacgat cggattgact ttcgaaacag cttgccaaaa
    40261 tggcggcaac atagagatgg catagaaaac gccacctaga taagttaacg gctgcaagac
    40321 aaatgtaggc acgatcgata catcgtcata agactgagca aaaatcccgt tgagcagtcc
    40381 tgctaacgaa aacaagattg ccgtcatcaa taatgtaatg atgaccatga accatgaata
    40441 cacatgaagt ggtacaaaga acaaggaaat gatcgttacg agagagccga cgagaatact
    40501 gcgacccact ccgccaatca caaagcccca aatgatcaca tgcgtaggaa caggcgctac
    40561 caagatttct tcgatgtttt tctggaattt ttgagagaag aaggacgaag aaacgttggc
    40621 atacgaacta gtgatagcag acatcataat caaaccaggc acgatgaatt ccatatatga
    40681 aaaaccaccc atgtcaccga tccgaccacc gatcattttt ccgaaaataa caaagtacaa
    40741 ggatgtcgtg atcacaggcg gaaccagtgt ttggacccag atacgcaaat agcggttcgt
    40801 ttccttgata gctaaacttt ttaatgcagt aaaataaaga ttaaacattt ttctcctccg
    40861 tatgattatt ttcttctgtg attttcaaaa acagttcttc taaacgattc gacttattcc
    40921 tcatagaaag aactttgatt ccttgtcttg tcagctgatc gaagattccg ttcacccctt
    40981 gatttcgttc aacttcgaca gcgattgtct gttcatcttc aaatgtacaa gcgtatcctt
    41041 caatcacagg tttggtctca tatggcgcta aatcaaagat aaatgtttca aattgcagtt
    41101 tggatagaag ctctttcata ctagtattct cgatcagttc acctgattgg atgatcccga
    41161 tgttgcgaca aagcatttca gcttcttcta aataatgcgt cgtcaaaatg atcgttgtac
    41221 cattttcgtt caattctcgg agaaactccc acatctcgcg tctcaattca atatcgactc
    41281 ctgctgttgg ttcatccaag atcagcaaac gtggttcatg catcaatgcc ctagcaatca
    41341 tcagtcttcg cttcattccc ccggaaagca tacgtgcacg gacatttctc ttttcccaaa
    41401 gattggactg cttcaaatat ttttcgctgc gtttcatcgc ttctttgcga gagacaccgt
    41461 agtagcctgc ttgattcacg acgatttgct ggaccgtttc aaagggattg aaattgaact
    41521 cttgcgggac taatccaatc tgttgctttg ctcggactag atccgtatcc agatcataac
    41581 cgaagacatt cacttttcca gatgttttat tgactaaaga agtaatgatt ccgatcgttg
    41641 tagacttccc agctccattt ggtcctaata gcgcatagaa atctccttct ttcacggaaa
    41701 gatcgattcc tcgcaatgct tcgaccccac ttccataaac ctttttcaaa ttatttattt
    41761 caagcgcatt tgccatttat ttcctatctc cttatagaca tgattcttta ttgtatcatg
    41821 gaatacagca gatgaaaaaa caacttggtt ccagcgaaaa atcaggttgc tgggctcagg
    41881 aaaatctcct aatgaaagac atcaatcgga cgaagtcaga caaccttttc agaaaaaaca
    41941 aatcgaagat aaaggattac tattcatatg tgcaacaaaa ggagcgtgag acaaaagtaa
    42001 agaacacttt tgtctcacgc tctaaacacg tataaacggc ggaagcaaaa gccaagattt
    42061 ttgatccaat aaatactttc gatcacttcg cctatcccaa tacatgccac tgccgctttt
    42121 catcaaataa cgcggcttcc gtttttgcgg tttctactaa agtctctggg taatccaaaa
    42181 ctttcagcaa ggcaatcgca tttcgggtcc ttgaaggtcc ttcttttaat ttgaaatcaa
    42241 aactgattcc ttttccttct gttacttgtt cttcaaagtg gacattctca caataattct
    42301 ttaaaatctc tgtcaattcg atatcatgtg tggcgacaaa tgctaaagat ggatactccg
    42361 caagccaccg aacgactgaa gcggaagaag caatccgttc gatcgtattt gtccctttta
    42421 aaatctcatc tacgaagcag tagcagcgtt ctttggaagc cacttggtcc aacaaacgtt
    42481 tgatcgattt gatttctgaa acaaaataac tgtcgccttc aaataaatca tcttcgattg
    42541 ccatggaagt caacacatgt ccaggttgca gggtaaactg ttctgcaatc gctgtttgga
    42601 tcgtctgtgc tagaatacag ctgatggcta tacttttgac atacgtagat tttcctgatg
    42661 cattggaacc cgtgactaat gtattcttct gccagttgac cggattcact accgcttctt
    42721 ttaacaatgg atgatagata tctttcgctg tcacaccgcc ctcagtaaac tctggtcggc
    42781 aagtgatggg catataagta cggtaattca atattgcagc ggctacttct aattctccca
    42841 gcaattccca cagcttgatg gcttcagttg aatgcttttc taatttagtg agaacgaaat
    42901 gataggagat aaatggcagc atgaacatga tgttgacata ttcaaaaatc aactctgctt
    42961 cactcccact cttcatacga aatgaaaaac ctaatttagg gatgtctctt aatggtttag
    43021 tttgctgttt cagctcttct tgaagtggtg tcggcagttt tgctagctga ttggcggctg
    43081 aaatcgttga aaccaaataa ctcatgctat tcagctctgt ttctagtgtt tgtttcttca
    43141 ctagataata gaccgtattg aatactaacc caccgattgc caaatagatt cctagtactt
    43201 gtcctaaaag aagcaaaccc aaacccacca gcggaagcaa gccaagaaaa ataaataaac
    43261 ctacgcgtcc aatcttttta ctttttccat ccgctagata ttgttttgca aaattatgat
    43321 ctttttttcc taaacgagcg aattgatatt ggattttttc tctcagttga ggattttcct
    43381 gataaaaagc aatcagtttt tctaactgct gatcttcacc aaaatcaaag ctgcgtaaac
    43441 gttgatacaa cgcttctgaa cctacactag aataggtact gttgattcct tcgaatactt
    43501 cgaacatatc caggtcatac caagtaagat catcgatttc gctatcccat gaacggaact
    43561 tcttctccgt gagccatgct tccttcaagc tttcttcttt atcaaatctc ggttgataag
    43621 gaattttttc ccatttgcta cgaactgttt cctttaattt caaacgtgaa aaaatgtcta
    43681 aagcaactaa taaaataaca gctgcaataa taattaaaac gataaattct ctttgaccca
    43741 tatccctctc cgttccttta tttgttagct ttagtatagc actgttgcgc tatttgaaaa
    43801 gagggtaaaa aaacaccttt caaaataaaa ggtgtttttt tcttgtatat tacatattta
    43861 tctctcattt atcaaaattg acaaataatc atagggagga attgttgtta attcttgttt
    43921 attctccagt gcattgacaa ctgtcccaag ggaaaggata gcctcaggta ctattttttc
    43981 ttgttctgtt ggattagcta agcgaagaac aaatccttgt tcatgaaaat aagaaggata
    44041 aatcgatgat acaattaagt cttcaggtaa ttcaatcaat gaaaatttac gaggaacatc
    44101 aagttcattt tgtaatggcc aaatcttgtt atctaacctg tgaataaata cattcagttt
    44161 ctgcttttga taagaaatag attgttctaa ccgtttttct gcctgttttg ccactttgaa
    44221 ctcattaaat cttccttctt ctacattaaa tccaaaagtg actttatact cacctatctc
    44281 ttgagctagc ggtgtttcca tcatgacatg cccttcattt gtcgtatctc ctgaagctcg
    44341 cccaggtcgc caagcaagat taggttttcc aagttcccct gttgttgaaa ataaggttat
    44401 gattaattga ttttctatac gttcatactc ttttaaacca tctgcataaa acgtaaaata
    44461 atgatcattt tctgccacag agacactctt atcgaatatt tctaaattaa caggtttctc
    44521 taccatagtt tctgaccaat tcggaggaat gacatcaggg gtattttcaa taaatccgtt
    44581 ttgaatttgt gcaattgatt tttcagattt atctaaaaca ttaattgcta agcgcatacg
    44641 atgcgagtaa acagtattat gaattttgag tgtgccttca acgatttgac tttctcccag
    44701 actgaacgat atttcataag atatttcttc catttcgggt ttttcagata aacgatcttt
    44761 taaatcttta ggaagttgag cttttccgta aacaactaat gtttcttgta aagaatcttt
    44821 atatacttta gctgtttcta attttagaga tagttctgta tctccttcta atggtgaata
    44881 atcatacgta tcgccatcat ttgctgaatc gatcaaatga acgaaatcaa gatattgtct
    44941 atctccaact atcaaattca attgcccatc tttaaaagaa agtttatatt tattgttact
    45001 tatctcagtt ttgtctatct cgattgctgt ttctaatttt tcattatttt tctcaaattc
    45061 aacaacagtg tatcctagtg ctggcacatt tacatttatt tttatgtcaa gttcataata
    45121 agctggttca tcaaaatatt cgaaacctgt agcaatcatc cgataagcat ttttccgttc
    45181 tggaaaataa acttcttttt caatgatatt atagggacta cctttgaagt gtattttctt
    45241 tgaatcagct actacatgca cgattttcga tccactataa ttctcagtcg ttgtgttgaa
    45301 aacaaggact tcattcgatt ttagctttaa tgcatcagcc atccgtttta caataaggtt
    45361 ttcgattccg tcagctatct cattagcttc tttgattcga tggaaaatat cttccgcaac
    45421 attatctgaa acacagccac caatactatc atgagcctgg ttttgcaaaa tctttttcca
    45481 taaccgacga agtaggcctt tactgatatg gataccattt ctttctgcaa tcagcatgag
    45541 cggctcaact cgttttagaa ttttttgttc caatttaaaa ttttctattt tcatttttgt
    45601 tcttactgag ccaatagaac gatgaacacg agagtaaacc ggctgacgaa gttcaccttt
    45661 atattcaggt aattcttttt tgtttctgat aacttctaca taagaaggat aatctgaaat
    45721 aatattgtgg aatttactgt atttattcaa cttctctacg acttgatcaa aatttaaaat
    45781 cattgacttt tgatctacac ccgaaggtac taaaatatct ttgttttttc cttgcatatg
    45841 aagcatttca actgcttcat ccaacttttc ttttgcaaat tcaggaatat ctttaattgc
    45901 gtctaaggtc atcattccag tcatataacc aaatggaaaa ttcatggcat aaacaaattt
    45961 atctcccaat cctttccaga taaaataagg agatggaacc atcgtttcaa agtttaatcc
    46021 tcgccaacac atgaaattat cgatttctac ttgatgcaaa agtgtaggca aattcgcatt
    46081 gaacccaaac gtgtcaggca aataccctac catcattgga tctccatatt taaggatcgt
    46141 atcactgatt ccaatcatta agtttcttaa aattgattca gcatccacta ataaggcatc
    46201 tgtttgtgca taccacggtc ccacgaatag ttgtcctgtt ttcatcagtc gttggataac
    46261 cttaagcttt tgcggattga tctccaaata ctcatcgacg attgaagttt gtccatctaa
    46321 acaaaagttt actttagggt tcctttccaa ttcatctaga acttcagtaa agacctgatc
    46381 agacaatacg atggcatctt catttgtaaa ataccattct ctgtcccagt gcgtgtgttg
    46441 aattatatgt gcatttacca tatcagattc tcctactttc cttcttctac tgaatttact
    46501 tgttgttctt ttttatactt gatggatttt agtgcattga ccattaacgc tgtaaccact
    46561 cccccaataa caattgatac taaatataaa ggtatattcg taacaaaagg aattgccata
    46621 aaggttgaaa gcattgtttc ttgagtaaca ccaaaaaatc catttagacc acatgctact
    46681 gcagtgccca ccacgatgct tggtattact cgtaaaggat ctttcacggc aaacgggatc
    46741 gtcccttcag taatccccac aaggcccata atcatagctg tctttgcatt atctcgttcc
    46801 gattcatcat aaagtttttt gtttaaaatt gttgctaaaa acattccaaa tggcggaata
    46861 gcaattccta ataacatggc tgtactaggt ccaaagcttc cgcttgaaaa agaagcaacc
    46921 ccaaaagcat atgcgatttt attgataggt cctcccatat caaacgccat cattcctcct
    46981 aaaatagccg acatcaaagc agcatttgct cctgacatgc cttctagcca attagtcagt
    47041 cctgttgtca ttgcttgaat aggcgtagct agtacatacc ataatatgat tccaacaact
    47101 aatgtagtaa caaaaggaat cagcataatt gattttagac taataacttg catcggtact
    47161 tttacttttt tcaatgcatt agctaaatag ccacaagcta atccagcaac cattcctcca
    47221 ataaatccca taccaagatt ttgacacatc atcccagtga taaaagctgg tgctagaccg
    47281 ggacggtcag caattgaata tgcgatatag gctgaaatca tcggtacgat aaactgtaat
    47341 cccgtctgac caatcattcg caggactccc cagaatgtat ccgtctggtc ccaaacacct
    47401 gtgccaccaa aaatgactgc aatagaagtt agtaaccctg ctgctacgac aagcgggatc
    47461 atataggaaa ccccactttg gaaatgattt agaatttctc ttccttgttt cttgaattcc
    47521 attagtaact cccccacgat atatttttta tttgttaatt aatgcaattg cttcatcaat
    47581 cgtctcttca ccatctttta ctacctctga ggtccctact gttaaaatcg gtttaccgtc
    47641 aaagcgacct cgttctgaaa catttgtatc tactgcaaat atgacaacat ctgcctcgga
    47701 aacatccgaa attgaaagtt tgttttctat ccccatagcc ccctgtgttt ctactttaat
    47761 atctaaccct ttcttcttcg caaacttttc taaattagct tttgccatat aagtatgggc
    47821 aatccctgcg atacatgctg ttactgctac tatttttgtc atatgaaatt cctcctttta
    47881 aaatgataga gcttttacca attctttttt atttctcgaa tcttttattc ttttaacaaa
    47941 ctcgtcatcc atcaatcttc cagataattc tgctaaaata cgtaaatgat tagtaccttt
    48001 atcagtgtct tttattgcta acaaaaagac gagatcaact ggttgatcat ctaatgcgcc
    48061 ccattcaaca gaccgcttta acttggctac agccactgtt gattcaatta ctgctaggct
    48121 tttgccatga ggaatggcga tatttttccc aattcctgtg gtcatatggt tttctctgtc
    48181 aaatacgtct ttttgaaatt gtttgtggtc agacacaaac ccattagtct ctaacaattt
    48241 cgataatttt tcgactacac tttttttatc agatgcattt aaatctatca ctatattatt
    48301 tatattgatt ttttcactga tcatttataa gccttctttc aatatataaa ctaatttttc
    48361 aaacgacgaa ctcgttttta aatttgctaa taactcatga ttcgaagcga tttcattgaa
    48421 tgtttcataa attttattca attcaggttt tactttttca ttaagtgcaa aaaagaaaac
    48481 agaatgaaca agtgtcccat cccaatcaat tccttctgga ttaacgtaga cacagattac
    48541 tggctttttt ataaaatcta cttcgatatg cggcatagca aaatcattca tcgctgttga
    48601 agatagtttt tctcttttga ttgctccctc tagtatccct tctttcgcgt aattttcttt
    48661 taccaatttt ttggtaatta tatttaaaac gttcacttga ttatccttta tttggtgggt
    48721 aaaaaagcaa aatcgtttat ctaataaatt tagaaaagca tcttctgttt gttcatttaa
    48781 ctgtagatac tgttttactt ttctgacatc tgcccctaac atcaaaggac taacaacaat
    48841 aacaggaaaa tttgtatttt caatcggaat cgtgcttata acaagttcat cagtgacttt
    48901 tctatcttgt agttcattga tgcttagtac atcttctatc tcaaccatat ctgcaaaacc
    48961 gttatttatt ctttgctcta ataattttga tgttccatat ccgctactac aaactaatat
    49021 cactttcgtt ttgtcaggtt tgtaacgatc caaaagtgat tgaatatgga gtgttacgta
    49081 agcgatttca tcttcattaa aatgtataca gaatctctct tcgatttcct ctaataaatc
    49141 tacacttatc ataaatgacc ttttaaaaga atatttgatt ttgtccgtat aagggttata
    49201 gatatttact cccaatttga gtcttttaac tgctgcattg agatgtaata ctagattatt
    49261 gatcaattct tcatcaggat aaaattcatt taatttaatt cttactaact cttgtagctc
    49321 aacacccata tctaaatagg aaagcttcgt ttgatctaag tcgtgattca tagcattttt
    49381 ttcaatcgaa gtaatgtgaa tgtcaacata agcttgttca tctattggca caataattga
    49441 aaatgctttt tcaatcattt tgattaaaat aagtgtattc tttgagattt cttcttcaga
    49501 attattacta cgtgataaga aattgcttgt ttcaatcctt tctatcgcaa tagcaaggtg
    49561 gatagctaaa gtttgaaatt cataatctgt aaatttcaat tgtgtcttat tcatgtatcg
    49621 attaagcaat tcggctactt ttttcaatat gtccttattt aagaaattat tcgcttcacc
    49681 aatgaaattg atattcaagt ctaagagatc tgttttactg ttagatgctg taaagccacc
    49741 ccaataatag ttcacaactt ctgacatcaa ccttctttta cttttttcat cagctttgat
    49801 gcgcacgcca tttcgatctg aaactaagga catcccatgc ttgtttagtc tttttctaac
    49861 atctttcatt gtgctttcta ccgtcgacct gctcacgtat aattcgtcag caagattttg
    49921 aatctttaga gaatcattag tattcaataa tttagcaaac acataagtta gacggtcctc
    49981 cggagagtct gcgatattga ttttatgact ggataataaa tttcgaagtg tttctttttt
    50041 tccaataagc gaaacgcctt ttttaggttg aaccaacatt tgtacatctg gcattttttg
    50101 gattatttct ttcaaaatta aaatatcttt gctaattgtt ttcgtagata catataattg
    50161 ttctgataat tctttgtaag ttgtgtgctc attttcaatc aaaaagtata gtattttcat
    50221 ttgccgttcg ctaaattcga tgcccattat cttcacctcc tgaatacagc ttaagtgtac
    50281 aattattatc tctattttca tacagaagaa agttcttgat taatgtagaa gtttctaata
    50341 ttcttgatta tttcaaatca ataaaaaagc ctgcaatatt tatatcacag gcttttttct
    50401 ctattaaata tatataagat aatacatttt tctactagtt ctcactaatc ttgtataatt
    50461 aaaccctccg caacttgacg gagatggaga agatgccccc gtctaatcta gcggtcatct
    50521 ctcctcccat tccttccacc aatttttttg acaggtaaag cccgattccg ctagattccg
    50581 tatttgtgcg gctggtattt tcggtataaa aacgctggaa gactttttca acagaggttt
    50641 gcgttcctgg tttgatagga ttggccactg ttaatcgaat gaatggatcc tcttttgaaa
    50701 atgtcacctt tgcttgtcca tctgcataac gcaataaatt ctgtatgata ttttgcagca
    50761 ctcgcagcaa catgagcgga tctgctaata cagttgtatt tatcgagtgt tcagcgaacg
    50821 ctacctcgat atttttctca gaaaattgtt cataaaaaga gaaaaatact tcttccacct
    50881 ctttagaaaa agaaagcgta tggagatcca tttctttttg cttcgtctct attcgagcaa
    50941 gatcataaaa ctgatccgtc atactgagta atcttgcaac agattgtttg atgatcgtta
    51001 aatacttctc ccgtttctcc gtatcctcta cctgttccag taattgtaca taaccgttaa
    51061 tagcagtcaa aggagttctg agatcatgag aaaggccgac gatcgatagt ctgatgtttt
    51121 cttccatttc ttgaaatgct tgtccttttt gttcattttc atccaaccat gtattcagtg
    51181 catctactag gtccattaga ttcttttctc gaaattcgac tgaaagacga ccaccatatt
    51241 cttgtcgttc agaaagagtg gcgattttct tagttaatct cttgatttcc ctttgcagga
    51301 agaaaataaa acttcccata aagataacaa cgagaagcaa cagccattta atcaaatcta
    51361 aaccccactc cccacaccgt atgaatatac acagcatgtg cctttgctag cttccctcga
    51421 agattggaaa tatgcatatt cactgttttc tcactatcaa aatatggttc ttgccaaact
    51481 gtttcataaa gatttgctcg cgaaaatact tttttcggat gttctaaaaa aagcttcagc
    51541 agatcgaatt ctcttcctgt caaatggaca gccacaccat tcacatttac atccctcgac
    51601 tctttgtcca gtaagatttc cttgtaatag atgcgttgcg acagatcttt tacttggaca
    51661 gaagtctgat gacgaagttg gatgccgatt cgtaacaaaa gttcttcgat atcaaatggt
    51721 ttcgtcacat agtcatccgc tcctgctgcc aacaaggaaa ctttatctct ttgatcgctt
    51781 tttgctgaaa gaacgatgat cggaacattc gatgtctgtc ggatttctgc taacaatgct
    51841 tcacctgaaa gccctggaag catgagatct agaaggatca aatcgacagg atatgtgctt
    51901 aacaataatt ttccttccgt acctgaataa gcatcaagaa gtttgtatct ctgttcaaga
    51961 acctcctcca gtaaggaatg aacagcttca tcgtcttcga taatcaaaat agtttccagc
    52021 aggaccacct acttaatgtc agatttttgg aaaataaaca ttcctagagc tgatgatacc
    52081 acgattgtta ctagactgac cagaatcatt ttcggtgctt gcgaataagt tgcttgttgc
    52141 gcaaagtccg tatacatgag ccacgtttcc gtaaaccact ccgttagaaa ttccaagcct
    52201 ttgatttttg cgaaaacacc gggaatgaag gccgttccca tgatataaag aaaagaaaga
    52261 agcaaactga cactggtact tctagtgatg aatgtcaaag ccactggaac agataatgtc
    52321 cctaatgtca gcagaagcaa tacaggaaat acttttagaa cataatttcc atagtttttg
    52381 attccctcaa cttcaccaaa tccgaaagca agtgtgtaag caagcatggc tagtcccgtg
    52441 atcaaaacca cgccaagtgt tgtgatcaaa agcgtaacga gaagtttcga cacataataa
    52501 gtcattcgat tccgtcctaa agataacgta ttgcgaatcg taccgttttg aaattcttcc
    52561 ccaatataaa agcacgcaaa tgctgcgaaa gccagaagaa tccatgcagc cattttttct
    52621 accattaaaa tccctgttgc accgttgatg actgtatccg ttgcttggtt taattcattg
    52681 ccgtattttg tatgcatata ccattcgttc caagaataaa aaagcggaag tacgaccatc
    52741 agtcctagtg agacccacat tttacggctc ttccatactt ttattttttc agcagttaat
    52801 agattaaaca tgtggattgc ctccagttaa ttgtaagaaa taatcttcca gtttttgccc
    52861 aactaggtga ataccatcca caggaacttt tgcttctacc aatgcttgat tgatggctgc
    52921 tacttggtca attccttcaa aaagctggat aacacggcta gattgtacaa aataatccct
    52981 ataaccaaga gagtcgagaa ccgttactgc ttgttccgtc gccgaagtgt caatttttat
    53041 aaattgacga gattcttgtg ccaattcttc tttcgataat tctttgatca agtgtccttc
    53101 atgcaaaata ccatagtgag tggcgatctg tgacaattca tctagcaagt ggctagaaag
    53161 caagacggtg atgccccgtt cttttaccaa acgttggatg atttctcgca tttctgccat
    53221 ccctgctgga tccatcccat tcgtcggttc atccaaaatc aaaaattctg gattcgtgat
    53281 cagtgcattt gcaatagcta aacgctggcg catacctaaa gagaaattct ttgctttctt
    53341 tttccctgta ttttccaatc gcatcatttt cagcaggtct tcgatttctc tatccgacac
    53401 accgccgttc gctgcttgga tacgtaggtt atctcttgca gttaattctg gataaagagc
    53461 aggggtttca atcgattgac ccatacgtcg tcttgctgcc tgcaaatccg tcgttccaaa
    53521 taattcgatc gaaccttcat caatcgagat caaacccata atagttctca taaaggtaga
    53581 cttacccgct ccattttccc caatcaatcc atagatcatt ccacgtttga tttcgattga
    53641 tactttatct aatgctttgg cagcaccgta ttttttggtt ataccagttg ctttcaagac
    53701 cgtttcattt ccaaaatcca tacttatcct tctttcttat tgatgagtga agtatagttg
    53761 acgggtataa agaaagcagc cagaaatgta taaagaaagt gtaaattttc caattgaatg
    53821 tcaaaaaaaa caatcggaca atacgtgtct ttcctattat caggcagttt gttatacgct
    53881 tgtatcagtt tcctgattct ttcttttcga ccaaagtcgt ttctctgtat tcctcttcac
    53941 tgaaaatttt tgcttctttc agatcaagct tgagtaattt taccagaccg gttataagga
    54001 taactatagc aagactccct cccagaaaaa ttagactgtc tcgcagacgt ggttgcgttg
    54061 tcttccaatc aaagagcgca ccaaacagtg ctaatccaag tggactggcc accatggtaa
    54121 tcgttgttat cgttgacata agtcgtcctt gtatatgcgt tggaacttct cgctgaagcc
    54181 agaccatata aaaagcaaaa gaaaacatta atccttaaaa acggacttat gaaaaataat
    54241 acatgccgtt tgggatttac aattagctac caaataagag gttatgacaa aagacttttc
    54301 acaatccctt cttccgaata aacggtgttc tcctgccaac tcctcggtca caagtcacaa
    54361 tatttatcaa acatgagaag caatttttgt aaattccttc ttatttcttg aagctgacca
    54421 cttctgtcac aacctcagaa gcggataaat aatattgttt gatgatactt tatctttttc
    54481 ccgtttgttt atagatcgaa tgatattata gaccaagaat cctacctatt aacatagcta
    54541 aaaacacaaa aaaggcactt tccaaattgt ggaaagtgcc agaaaagtta ggattaaggt
    54601 aactgcgctc agcattgctt cgcccagtac gattctcgta actcgtgaac tcgaacgata
    54661 atcgcaatta acgttttgag aactgtgaag ctttacgggc tttcttaaga cctgtttttt
    54721 tcttcgtatc attgaataag tttgaaaaac cttgatttat aaaggttttc ccgtaaatat
    54781 attttattat atttgattaa atttgaaatg tttgtgttct aaaatgtgtt ctaatttaaa
    54841 ggcagtatat ttcgtatttt agattaaatt tttaggctca ttcattctca aatccattct
    54901 attcagtcaa taaaaatttt aaaaagactt tataaacaaa gatgttcaag cgagcatctt
    54961 atttttttgt gcttaatttg aaaggagaac atttcatgta taaaaaacaa aaaaagaaat
    55021 tatgtgtatt aactattttg agtttggctg ttcaagcaat tgttggagca attgctccta
    55081 cgattacttt tgctgatgaa attacccatc cacaaacggt aaccgtagaa ctagatttag
    55141 cacatcaata tgctgttgaa ggaactttta gtgatggtcg tccaatgtca gaagtcactg
    55201 ttccgcatta tgcagtgtac aacggcttta aacaagatat tttctgtatt gaaccaggag
    55261 tcccgattta caatgagttc actcctggct atgagaaaaa cccgttacca gatatgtccg
    55321 ataaagccaa attagtttcc gttttatgga aaaaagtagg tacagatgca gatactcata
    55381 ttgtggcaca gaagatgatc tggcaagaag ttaatgggta tacccttcac tcaataaaac
    55441 gatcagatgg tagtgccgta aatatcgcag ccattgaggc aaaaataaat caagccattg
    55501 acgattatca gaagaaacca accttccata atagtacggc taaaacagta ttagggcaat
    55561 cgactactgt gacggatacg aataatctga atttgtcaga gttcgatgag gttgtggaaa
    55621 atgccgcaaa cattgattat cgtgtgaatg gtaatcagtt gataatcacc ccaaatgcca
    55681 attcaaaaga aagtggtgtg ctgacgctta agaaatctgc tggtaccgga acacctgtcg
    55741 cctataaaaa ggctggccaa cagactttaa tggccggggc gattgataaa cctaatacct
    55801 acacaatcaa aattgatgtg gaaacggagg gttctttaaa gatcaaaaaa gtcgataaag
    55861 aatcgggtga tgttgtacca ggaacggttt tccatttaga ctttggaaaa actttacctg
    55921 caaaagacgt gactactgac aaagaaggca ttgctacatt ggatgagatt cctcatggta
    55981 caaaagtaac cattaccgaa aaatcagtac cggcgcctta tacgattgat accaccccca
    56041 tgactaccac cattaaagca ggcgaaacca tttacgtgac ttcaaaaaac gcacgggaaa
    56101 aagggcaaat cattctggat aaaagcggag ttgaaacagg gagtgatctt tggaatgaca
    56161 attattcctt agctggtaat acgtttgcca ttcgcaaaga cagtccaacc ggtgaaatcg
    56221 ttcaagaaat gaccacggat gaaaacggtc atgcggaaac accaaaagag attgctaatg
    56281 cgttggaatt gggaacctat tatgtgaccg aaactaaagc tagtcatggg ttcgtgaata
    56341 cgttcaaacc tgtaaaagtc gaattgaagt atgccaatca aaccgtagcc cctgttacca
    56401 gtaacgtaaa agggcaaaat caagaagtca ctggcgaaac cactttgacg aaagaagaca
    56461 aagatactgg ggataaagca caaggtaagg cagtctttga gggtgctgaa tacaccttgt
    56521 ttactgctaa ggatggaaaa gctgttaaat ggagtgaggc ttttaaacca gagttagcga
    56581 agggaacgaa aggttctgat gaaacggtca ctttgacttt agacgaaaag aaccaagttg
    56641 ccgttaaaca tttagctatc aacgagtatt attgccaaga aacaaaagcg cccgaaggat
    56701 atagccttga tgaaacaaag tatcctgtat ccatcaaaaa ggtggatgat agcgaaaaaa
    56761 atgccgtgat tacccgaaat gttacggcaa aagaacaagt gattcgcttt ggctttgatt
    56821 tctttaaatt tgctggatca gctgcgggca ctaccgaaac tggcttcaat gatctgacct
    56881 ttaaagtgtc gccattggaa gggaccaatg aaatcacagg tgccgaagat gaagcgacca
    56941 cagcttataa cgagcaatta gggtttgatg gttatggcaa gtttgaaaat cttccttatg
    57001 gggattattt acttgaagaa gtagaagccc cagaaggttt tcaaaagatt acgccattag
    57061 aaatccgttc tacttttaag gaaaacaaag aagactttgt gaagagtgag tatgtcttta
    57121 ccattaccga acaagaccaa aaacaaccaa ttaaaacggt gaccgttcct tacgagaagc
    57181 tgacgaacaa agcattttct gttagtttga accgtttaat gctctatgat ttgcctgagg
    57241 aagaagatag tttaacttct cttgcgactt ggaaagacgg aaacaaagaa ttaacttctc
    57301 tggattctac cgagctagtt gataaattga gttacaatct acatgaaatc aaagaagact
    57361 ggtatgtggt agcgcaagcc attgatgtgg atgcgacaaa agctgcccaa gaaaaagatg
    57421 aaaaagccaa accagtggtg attgcagaaa caagcgcaac gttggcgaac aaagagaaaa
    57481 caggaacttg gaaaattcag cataaattaa ccgccgaaca agttttaaat aaaactattg
    57541 tcttattcaa ctatgtgtat gaaaataagg aagcttttga agcaggcgat aagccagtag
    57601 cgaaggatgt tagcttgaat aaccaagccc aaactgtcag ttgtacagtg gaacaccatg
    57661 tttctatcca aacaaaggcc cacctagaaa acggttctca gacctttact catggtgatg
    57721 tggtggatat gtttgatgat gtgtcgatta ctcatgatgt actggatggc tcaaaagaag
    57781 ctttcgaaac aattctgtat gctttattac cggatggtac aaacaaagaa atttggaaat
    57841 ctggcaagat tgattatgaa gtaaatgata aagaattcac caaaactgtg cttgcgaaaa
    57901 aagtagatac cggaaaatat ccagaaggaa ccaagtttac ttttgcggaa atcaattacg
    57961 ataaagatgg gaatatcaat ggaaaacaca atgaagattt gaaagaaaaa tctcaaacct
    58021 taacaccaaa agaagttcca accatactaa gtacgccgaa acaaccggaa acaccaactg
    58081 ttccaagtgg ctctcaagaa tctagtccca cagtgaagac cttccctcaa accggggaga
    58141 aaaattccaa tgttttactg ttcattggtt ttactttgat ttttgcgacg gcgggctatt
    58201 atttctggaa tcgccggaac taaggtgatg agatgataca agtaaatcct aagaaccaac
    58261 gtgcgcgacc gcctcctaaa aaagtagtcg cgctattttt gtatccaaaa ctaacaatga
    58321 aaagaggaaa agaagatgga attgaaattt gtcgtgccag atatggctga aacctttgga
    58381 aagttaagtt atgccggtga aggagaaatt ataacagaag gatatggacg gaatactacg
    58441 gtgattggtc gcagttatca tttgtattcc agtaagcaac gggccgatga tattgaggtg
    58501 gtagtagctg cggaagccgg tgaaaaggat tttgatcaag accaaccgct aaaagccgtg
    58561 aatccccatt tggttgccaa aggctatgag attgagaatc gtgggtttac tgattacgtg
    58621 ttgtatgttg atgatttagt gaaagcatag gaggaaaaga agatgagatt aacagaaggt
    58681 attgtcgtag attcaggttt aacatttggg aagttgcgtt tttccgcatt acgccgagaa
    58741 gtacggaagc aaaatgagga tggaacaatt ggtaatgagg taaaagaacg aacctataat
    58801 ttaaaatcct ctgcacaagg gcgaatgatt caagttagta tcccagctaa tgtaccctta
    58861 cgcgagtttg cttatgatgc agaggtagag ttggtcaatc caatcgtgga taccgttgcc
    58921 aattatgttt ttcgagaagg aactaccgtt aattggttca ttaaggcgga tgatttagtc
    58981 ttgaagcggc agccgaatca aggaaatcct acgaaccaaa atgaaagaaa gaaataggtg
    59041 aagattatgg aaattaatgt tgaacatatt ttagattgtc tcgatcaata tggaaaaggc
    59101 gagctgactg aggaacaact cgttaaagca ttaacctctg atgaaaaaat gtttctgatt
    59161 atacatcaag gtttacttgg agaaaatagc gacagtgaag aagactctga tgtactagac
    59221 tggttaggag aagaagagtc tttctttata gttgtcgaag taaatgaaaa cttatgtcgg
    59281 caggcagaat ccattttgga agaaattggt gtggagatgc cggatgctat tgaaggcttt
    59341 ttaaatcaat tggttgaaac gaaacaactc ccctcctcgt agcagtaaat gactagcata
    59401 actttttgaa attgcctaat gaaatcctat tgggcttttt cgattggagg tggaaaccat
    59461 caaaatgtac aaaggacatc gcatacgcgc aggagatcag cacttggttt atcactttgt
    59521 tcttggctgg ctactcgctc tgttcatcgg ttggatgagc gtcttttatt ttcaagagtt
    59581 tagacaattt gatatttcta agctatcact ttctactatt gaaatcgttt ggtctataaa
    59641 agatttagta tgtctgcttg gaagtctcgc tttttcagga gctatgatat tgctttatat
    59701 tcactttttt cttgatcatt ggcgaagctt gtggcatcga caaaagctag ctcggatgat
    59761 tctagagaac cattggtatg aagtgaaaca aactcaaagt gaaggctttt tcaaagattt
    59821 gaatagtagc cgaaccagag agactatcag ttacttcccc aaaatctgtt atcgaatgaa
    59881 agatggatta ctgtctattc gcgttcaaat ttcacttggg aaataccaag atcagctttt
    59941 aaaattggaa aagaagttag aaagcggact gtattgtgag ttagtagaaa aggaactcaa
    60001 agactcctat gtggagtata ctttactgta tgatatgatc gccaatcgaa ttgggataga
    60061 cgaagtagtg gcagaaaatg ggactctgcg attgatgaaa aatcaagtat gggcttatga
    60121 ttctttaccc catatgttaa tcgctggtgg cacgggtgga gggaagacct atttcctcct
    60181 caccattatc gaagcactat tgaagtcaga tgcagaattg tttatcctcg atcccaaaaa
    60241 tgcagattta gctgatttag gtacggtgat gcctcatgtt tattcacaaa aggaagaaat
    60301 ttctgcttgt gtggaagatt tctatgaacg tatgatggct cgtagcaagg caatgaaaga
    60361 aatgtctaac tacaaaacag gagagaatta tgcctatctt ggacttccac ccaacttttt
    60421 aatctttgat gaatacgtgg cttatatgga gatgttaacg acgaaagaaa gtgcggtgat
    60481 tttgaataag ctcaaacaaa tcgtgatgtt gggtcgtcaa tctggtttct ttctgatttt
    60541 ggcttgccaa aggccggatg cgaaatattt aggagacgga atccgcgatc aattcaactt
    60601 tcgcgtggct ttgggtcgta tgagtgaact cggatattcc atgatgtttg gggaggtcga
    60661 aaaaaacttc tttatgaagc acatcaaagg tcgaggttat gtggatacag gtggcagtgt
    60721 gattagtgag ttttatacgc cacttgtacc aaaagggtat gatttcttag attcaattag
    60781 taaattagaa aagatgaatg attgagtgat cttcaatact gactttatac ttcaagtaac
    60841 gtataatact tttatatagt aggaagaaag gggataatta atatggaaag taatgattct
    60901 tatttagagt atgcaaaagc aaataaggat aagtttattt cttatattat caaagggaaa
    60961 tcccccgaag ttggacgaga cgcagttttt atggcaggga gtcctggggc tggaaaatca
    61021 gaggttgcca ttggattagc tgagaattat gataaccatg tgattattga tgcggattac
    61081 tttcgcaccc agtttcctga ttataatgga aaaaactcta gtctctttca aaaagcatct
    61141 tcttggttgg tagagcaggc atttagatat gtcttagatc atggctattc ttttattctg
    61201 gatgcaactt ttgcaatatt aagtgctgaa aaaaatatct cgcgggcgtt gaaaaaacat
    61261 tttcgagtga cgattttcta tgtttatcaa gaccctaaaa tcgcttggaa atttacacaa
    61321 gaacgtgaaa ttgcagaggg acgtcatgtg ccgaaaaagg catttattaa cgcttttttc
    61381 aaatcaagag agaacattgc aaaagtaaag agtaaacatc cagatgtatt gcttcatatc
    61441 ataataaaag attatacgaa taatatttcc gaagttcatt atgctacgga taatctagaa
    61501 ttatttttac cactagggta tagtaggaaa gatttggagg ttcaattaca tgactgagaa
    61561 agaacaagta acgaaaattg taaagaaata caataagagc attgcggatt tatcggaaaa
    61621 tgctactgca aaagagttca aaacggtgat taaatatgta gccgatcagg ccaatgaaaa
    61681 acaacggaaa ttagttggat taaataaaaa atagtaggac aaattaataa tgtatgagcc
    61741 aagagcaaaa tgttcttggc ttttttcttt gcaatgaatg aggtaatgta tgagattttt
    61801 agatttattc gccggtattg gtggctttcg tttaggtatg gaacaggcga gccatcattg
    61861 tatcggtttt tgtgaaattg ataaatttgc tagacgtagt tataaagcaa ttcatgatac
    61921 aagtaaggag gtggaaatgc atgacatcac aagtgtatca gatgagttta ttcaatccct
    61981 cggaccagtg gacatccttt gcggcggatt tccgtgccaa gctttttcaa ttgcgggaaa
    62041 gcggcaaaga ttttccgata ctcgaggtac tttattcttt gaaatcgctc ggttcaccac
    62101 tcttctccga cctaagcttt tattccttga gaacgtccga ggattgctca atcacgaagg
    62161 aggggctacg ttcgagacga tcctccgaac gctggatgga ttggggtatg acgtggagtg
    62221 gtaggtgctt aactcaaagg cctacattcc ccaaagccgg gagcggatat tccttatcgg
    62281 acattctcga gacgcatgta ccgaacaagt atttcctatc attggaccgt cttcaacatc
    62341 tgatcaaaac atcagaaatt tggtaaatat caatccttcc aatcgaggaa tgggtggaca
    62401 agtctatggt tcagatggcg tagcgccaac cttaacaggt gatgaaggaa tcaaaatcgc
    62461 actgccagca agggatggaa tctctgtagc aggactatta ccgggaaagt ttgaacaagg
    62521 taatcgagtt tatgaagtta ctggaaccgc ccccaccttg tcaacgaaac aaggtggtac
    62581 aaaaatcatg attcgaaaaa atgaaacaga atatcaagag gtgaagccag aagacagtgt
    62641 gaatctggct tttcctaatt ccacaagtcg ccgaggtcgt ctgggaaaac agagtgttca
    62701 taccttacta acaggagacc aacaagcgat cgtcacggat cagtatcaaa ttcggaagct
    62761 aacacctaga gagtgttggc gacttcaggg ctttcccgat tgggcttttg atcgagattc
    62821 tcaagtcaat tcagatagtc agttgtataa gcaagccgga aactctgtaa cagttccggt
    62881 catttttgat attgccagaa gattaaaaga gttggatgta catggtttat gatgagttga
    62941 ttggagaaat ttattgggtg attggaaaga ttcagtcaga cccagaatta gaagaacaat
    63001 tgcgacggtt aaaatttgat attcgcaaga atggtgtcaa agtcgctggc gatccatatt
    63061 taatgaatga ggagacggat gctcgaattg aaataaatca agtaattgca gaatttgagc
    63121 ggattgcgga ccaagcaaaa gaaccagata ttcgtcaata cttgttagaa atgaaagcag
    63181 agttggaagt aaatggtatt acagatgagt aacctaaacc aacacaactg actgttaggc
    63241 gctgaccgcg gaggattcgc gaagcagaaa tcctccgtgg tgctgtgcct gttcatggac
    63301 ggcgttagcc gaccatgatg tctcaccccc gtatctaaca ggggggtaca aaaacacaaa
    63361 aaataagtaa gcaaccatta agaatgcttg ttgtatcaaa gatagtaagt aatttgtata
    63421 ggtgttaact catttgaaaa agttaacacc ttgaatttgg aggtgagaac ttggcacaac
    63481 ggaatttaga ttatcggcta ttaaaagacc gacgcaacga atacggcgtt tcacaaaata
    63541 aattggctac ggcttgtgga cttagtcggc cgtacttaaa tcagatagaa aatggtggcg
    63601 taaccgcatc tataaaaacc atgaggaaaa tttttgacca actggaaagc tttaatcccg
    63661 atttgccttt gacgttactg tttgactatg taaggattcg ctttcccaca acggatgcac
    63721 gaaaaattat tcaagaaatt ctccatttga aatttgagta tatgctccat gaagattatg
    63781 ccttttactc ctatcaagaa cagtatgtca tgggtgatat tgtggtgatg ttgtctcatg
    63841 aagaagataa aggtgt
//
LOCUS       DASHHB010000011        59263 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.011, whole genome shotgun sequence.
ACCESSION   DASHHB010000011 DASHHB010000000
VERSION     DASHHB010000011.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 59263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 59263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..59263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.011"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(451..1761)
                     /locus_tag="U2K71_000770"
     CDS             complement(451..1761)
                     /locus_tag="U2K71_000770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEM7314876.1"
                     /translation="MRTKKGIINALNFKTNDTQQDDTIILQKAIDQGAKEHLPVFIPK
                     GIYLVGALFLKDKSHLIFEEGAVLKGRTDIEAFPEIDTRVAGVEMKWPAAILNVLSAK
                     DILIEGKGIIDGQGDHWWELYWGKDQKSGTRAEYDQKGLRWIADYAIKRPRACLLYHA
                     EHVVVRDLIFQKSGFWNLQITYSNDVLVEKVIIRNNDGPSTDGIDIDSSTNVRVYECD
                     LACGDDCIAIKSGRDGNGARVNRKSSRIEVARCKIRSGYGVTIGSEVSAGVSDVYIHD
                     IDFFQSDCGIRMKSSKERGGVIENIRVENLNMIDVQFPFSWIMDWHNEYNRKNSNDLA
                     QMPESWQAVAEEIPESKQMTKVKDIHIKNVQACLSPGYSLPSRAFDLVAFPEKPIEDV
                     CFSHCQITAKEFGRIEAVQNLCFKSCDLSIETGNTAANNTFDNR"
     gene            complement(1773..3191)
                     /locus_tag="U2K71_000771"
     CDS             complement(1773..3191)
                     /locus_tag="U2K71_000771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7314877.1"
                     /translation="MLSQNAVTANTIKKEKKEFGISDKIGYMFGDLGNCFILGLVNSF
                     LMIYYTNVLGVSGAIVGTLFLISRILDAFADVMVGRLCDVSELTETGRFIPWIRKMKY
                     PFCLVTVILFLPFVQHLPMTVKIAYIFVSYIVYGIFLSTINIPYGAMAAAISSNPNDR
                     TSLSTFRSIGSAIGGSTTGFFIPILMYTHNKSGQQVVSGNHFFWISIGCAAIAFVAYM
                     LTCKLTTERVRVVKNEKVSASQLVKGLLANRALIILVIVDIFIVINQILAGTNMTYLF
                     NDYFHNKQAMSVALLFTYGTVVVLAPFATKLTKLFGKKEASVFALLASSCMYLLMYFL
                     HITNAWVYLILLFIATLGSGLFNLMVWAFITDVIDYHQYKTGLREDGTVYGVNSFARK
                     VGQACAGAVGGFMLALIGYQSSTTGGAIQSVLVQERIYTMANLLPAFCLFVSAIILLV
                     GYPLNRSKTVFIGEELKKINER"
     gene            complement(3431..4999)
                     /locus_tag="U2K71_000772"
     CDS             complement(3431..4999)
                     /locus_tag="U2K71_000772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEM7314878.1"
                     /translation="MQTIKVKNPILRGFNPDPNILRVKERYYIIVSSFEWLPGIRVYE
                     SKNLVNWSHTTDILTTQVSLQGNPTNGSIWAPQISYADGLFYLVYTDVKSAGRPFKDV
                     HNYLITASEITGPWSEPIYLNSNGFDPSLFHDLETGKKWLVNELWDYRLDTPNKSSGI
                     VLQEYDAEKKKLDGPIYKIFAGTELAKTEAPHLYFRNGYYYLMTAEGGTGRGHSVTVC
                     RAKNITGPYELDPGFPMLTASDKPASTLQCTGHGSLVQTPSGRWYMTYLCTRPLNGAA
                     ILGRETAIQEVYWTSDDWLRLKDSQTTPMEEIMIETNEEVQQEKDHQFMDTFEGELKK
                     NWNARRILPNADWCDTKCRPGFLRLISGESLQSTFEHHLLAIRQTDFTFDAETAFEYM
                     PNNFNQMAGLVLYLNESNYIYCYVTWNEDKGKCLRMIESENGVAQIEDWTIPLTEKKT
                     FLKVTVKKETAQFHLKDVSTEEWKKVGPQKNMCILSGGFTGNFIGIAVHDLNKKRGSY
                     ADFEFFKYDGKDHL"
     gene            complement(5003..5413)
                     /locus_tag="U2K71_000773"
     CDS             complement(5003..5413)
                     /locus_tag="U2K71_000773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4064 domain-containing protein"
                     /protein_id="HEM7314879.1"
                     /translation="MKRKWELLLGMVGGSLSLIFFGGLAVTLSNMSASEFKKSYQSLA
                     VDHSTLSLENTFGLLQDMTGLFAVVLFISLAFLAVALFLTAKGKYLTTATGLYFITGF
                     ILLIGTQFIAFPFAFFYFAAGAFSLYRVRMRKGA"
     gene            complement(5410..6333)
                     /locus_tag="U2K71_000774"
     CDS             complement(5410..6333)
                     /locus_tag="U2K71_000774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pectin esterase"
                     /protein_id="HEM7314880.1"
                     /translation="MRMKDKLIIGKNAIDTPTDFETIQEGIDYLAGLPDSHPKVLIVK
                     EGRYDEYIESRLSNFKLVGAGTVVISGNRFAKQEYLDGSQLGTFRSATFFLEGSNVVL
                     ENLHIENTAGPGEKVGQAVALFNSGDRVSLKNCRLSGYQDTLCTGPLPDLQKDGTPFC
                     TPASANPEYCRQTYEYCWIEGTVDFIFGGADAVFHQCHIHSLASKNPGYITAASTPKN
                     QKTGYRFESCTLTAEPDTRNVYLGRPWRPYANVLFEACKIGGHVHPTGWDNWDDKKNE
                     QTVKFSERNNNYETSIKRPEWIHLEKQGVQK"
     gene            complement(6330..7208)
                     /locus_tag="U2K71_000775"
     CDS             complement(6330..7208)
                     /locus_tag="U2K71_000775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HEM7314881.1"
                     /translation="MQLNDFLGAPSSRHTPSGKRLYREKQNGFMLEYYQLYLNELETV
                     PALYAYPEKEGPFPPVLYLHSHGGDFSVGKSELIHGAPYLASPSFAEVLTGMGYAVWS
                     IDAWGFEERGGISESELFKQFLLTGKTLWGMRIYDVISLIDYLETREDTDTQRLATIG
                     LSMGGLLSWWVTALDSRVKVCIDLAAQVDIEILLLHRRLDHHGFYYYVPNLLTAYTTL
                     AIQEKIAPRPRLSLVGKNDTMCLDEGVLKLRKGLDKKYQSMGSPENFSSFSLTGGHME
                     TQEMRNIWKQFIREHL"
     gene            complement(7195..8532)
                     /locus_tag="U2K71_000776"
     CDS             complement(7195..8532)
                     /locus_tag="U2K71_000776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 28 protein"
                     /protein_id="HEM7314882.1"
                     /translation="MYDILTFGASIDELNTEAIQQAIDAAASDGGGTVVVPAGEFLTG
                     ALFLKSNVELHLSAGAVLKFSDDPKDYPVVHSRWEGVHRKVYASCIYAQNVENISVTG
                     FGTLDGNGKKWWHTFRNEPDNLAYPRPKLMSFHNCHRITVKDIKLIQSPSWTINPILC
                     SNATFDNLTILNPADSPNTDGIDPESCKNVRISNCHIDVGDDCIAIKAGTEDTYERIA
                     CENITITNCTMVHGHGGVVLGSEMSGSIRNITISNCIFQETDRGIRLKSRRGRGGIVE
                     DIRVSNIVMDNVMCPFILNLYYFCGPRGKEPYVWEKKAYPIDERTPAFRRIHFSNITA
                     RNVHASAGFIYGLAEQFIQEITFDNIDVSMAQDAVPGVPAMMTGIEEMSRQGFYIGFA
                     GKVSFNQMSIENHEGPAFLLENTKEISIEKFQSNQSENMDELVVRKNFAYAVE"
     gene            complement(8535..9638)
                     /locus_tag="U2K71_000777"
     CDS             complement(8535..9638)
                     /locus_tag="U2K71_000777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 88 protein"
                     /protein_id="HEM7314883.1"
                     /translation="MENWSKKLAKSIMERTPRLYEEKWYKGKWSYDYGVVLKGFQLLW
                     ERTQEKIYFDFIKDNIDYFVQEDGTIRGYSVEEYNIDHVNTGKLFFLLYKETGEEKYK
                     KAAELLSQQLANHPRTSEGAFWHKEIYPYQIWLDGLYMGSPFYAEFIQNFSEEKDYQD
                     VLRQFEISYKHLFDPKTNLLIHAWDEKKVQPWADPKTGLSHHFWSRSIGWYLMAAADT
                     YELLHEETDCSLLKEILEKTLAALLPYQEENSGTWYQVTTETKRKGNYLEASGSSMFV
                     YAMAKGIRLGLLNKEEWLPQVKKSHQGLLDEFVLETKEGWLNLNKNCQVAGLGGADQR
                     DGSYAYYISEPIICNDQKGVGAFLQALIEVEAL"
     gene            complement(9712..11220)
                     /locus_tag="U2K71_000778"
     CDS             complement(9712..11220)
                     /locus_tag="U2K71_000778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM7314884.1"
                     /translation="MKKATLVKLGLSFAGILALAACGSSSSNSSSETEDGKAKITWLN
                     ILHHASPPTDTIVNLIEEKTDTELTFNWIPDASKEERLTTALASDELGDIVTLQIAML
                     KNSSVRNALKSGVFWDVSEYLKDYKNLSQISEDRIEAASIDGALYGVPIQKDYARAGL
                     VIRKDWLDNLGLDVPTTMDELYEVAKQFTENDPDGNGVDDTVGFGDRAPSDIRYTSFK
                     LFTSYFGAPNGWAVDDNGKFTPEFDTEEYMKALNFSRDLFENGYLAQDFAVTQKTDQQ
                     EQFAQGRTGIYTGMVDIKNLKTMGQGIQDDMELVPVNKISDGEGGGYHVWSEGNGIGG
                     LLAFPKSQVKDEAQLKKLLQFVDDLLEEDVYKLMTGGIEGTHYELNDDGSIEFIDMDL
                     WQQEVQPLSSSRPSEVTFSFKDTDPEKEFSNQLIKENEEFAVMDPSVPLDSPTNNEVG
                     SEIEKIVIDATVQYIMGQLDEDGFKKAVEDWKAQGGDRITQEYEEAYKAAAQ"
     gene            complement(11186..11878)
                     /locus_tag="U2K71_000779"
     CDS             complement(11186..11878)
                     /locus_tag="U2K71_000779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEM7314885.1"
                     /translation="MLQSLDKINGRLNRILQLVYLNFLWGGLVIAGLGVFTFGPASYA
                     MVAVIRKWLRSKETFPLTKTYFTYFKENYRETLLMSWIYGAIGIVLLVDLSFIHNWYL
                     RIALYIAAFIYVLSALYIFPIMAHYDWKGYFFKMKMAFLFGFSHLHYSLVLILSMVGI
                     YLMIIQTMPGVLTFIGGSLFFSAVTWTANSIFCHLEWKNSYTEKTEENKNIIKTVKEK
                     IYEKSNARKIRS"
     gene            complement(11900..12874)
                     /locus_tag="U2K71_000780"
     CDS             complement(11900..12874)
                     /locus_tag="U2K71_000780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7314886.1"
                     /translation="MESVKKQEPFQLTLSKKEEKLRRRKKIIAGIKTNKFLYVMILPG
                     VLYFLIFRYLPMGGLVIAFQDYQPFLGIMGSPWVGFKHFIRLFTEPTFFTLLRNTLIL
                     FGLNIFVFFPMPIILSLLLNEVTNKRLKNGIQTIIYIPHFMSWVIIVSITYVFLNVDG
                     GVLNEFLATMGFEKISFLTSPEWTRTVYIGQVIWKELGWSTIIYLSAITAVDTQLYEA
                     AEMDGAGRLRKTWHVTLPAIRPVIITLLILKIGQTLDLGFEHMYLLLNSLNRSVAEIF
                     DTYIYTAGLKNGQLSFSTAMGLFKGVVGLFMVILANKIAKKLGEDGVY"
     gene            13174..14046
                     /locus_tag="U2K71_000781"
     CDS             13174..14046
                     /locus_tag="U2K71_000781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM7314887.1"
                     /translation="MKKKVSIGERSFTLFNNTFLILLALICIVPFLNIIATSFASTQE
                     VVAKKFILFPTTFSLDAYRYILSTPTIFRALAVSIGVTGVGTIVSMCATSLMAYGLSR
                     KYLFGRGFVNFLVVFSMLFSGGMIPTFLVVRSLGLVNSYWAMILPVAVNAMNMIIMRN
                     FFQALPDSLEESAKMDGCTDFGVFFKIMLPLALPSIATISLFYAVTYWNTYMTAILYI
                     NDSSKWPIQILLRQIVIVSSGMQAESSAVDVIPPAQTVKMAVIVIATVPMLIVYPFVQ
                     KYFVKGALVGSVKG"
     gene            complement(14504..16702)
                     /locus_tag="U2K71_000782"
     CDS             complement(14504..16702)
                     /locus_tag="U2K71_000782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7314888.1"
                     /translation="MKKSTVFYKIFLPMFLLGAVLVIGFSLFIYQNTYESIESSYLTD
                     KKNLLRQVKTNVEWKIRTIEYSFSTYGSTKNFSDIFKHPLTYTDYSTYSEIRKELNFI
                     ETIVMDDNNYDLVSLAGKWGVINGSLSQLTEEEVEKYQQKYINNKNNLFWQKKNKGIE
                     MVISLPMFEKEKYALGIATIEKHTIEQVVGESGEDILSIENEVGNLFTTQTVKNKEQP
                     TVLTDVDLDYNQLKVIREKDKTYIVLKSDYNNWIYRLEVDSSAIGAMIRNLRIGLVAV
                     SLTLVGLIGLLSYLFSDRFARPISQIQERLNLKSKGFSGKELDLVAQSVSRIIGEKEA
                     LSAHLVTQKPQLETLFVLSLFRNRVERRELDQRLQQFGYSSQNDCYYTALVQIDLLED
                     SHGGERDLLLLAINNMITEIVPKENRMLPIVLNEEMQATIYRMDKDDAEASKKVMEYC
                     RLIQKRVKEYLNLTISIGISNRFETLNESKQSVDRAKEALYYRVNTGPSSIIFYQEIV
                     PAAHEKTLIRYPIEEQNRLFEAIRSGNQEVKNLVHELIDALFAQNKNPLSREVVSVRL
                     INELVQLGQLLGVDSKNFDNMKQIYVKALNNYHPKELEKLVLEQLVLPITECSQAITD
                     HEFKNLSENIMHIVYTEYDRDLSLDVIADRLHYNPNYLSNVFKKETGEKFGDFVQNHR
                     LEVAKKWLKETNLSVKEIAERLQYRNPQNFIRFFKKKESITPGEYRKKYL"
     gene            16934..17926
                     /locus_tag="U2K71_000783"
     CDS             16934..17926
                     /locus_tag="U2K71_000783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7314889.1"
                     /translation="MVTIKDIAKAAGVSHTTVSRALNNSSLIKNETKQKIRQLAKEMN
                     YSPNYSAKGLVNQKKYLIGLFFSSLHKGTSSNFLADSIAGIHSVLDTNYSLSVESIDV
                     VDLTEINLQRYDGLIVMSQSDDDQPFIDYLKEKEFPFVVVNRHINDPAVINVTADDAA
                     GVMQAVDYGIGCGHTKIAYIGGKKNFRSTKEREKGVLKSLKKASIPVNPAYFLTGDYS
                     LESGLQNMEYLLSLSDLPTLVFCGNDDIAIGALRAVHLAGLRVPQNISLIGFDDSPVV
                     SYLNPPLTTVRKPLKEMCQEGTKLLLDLIEGRTIEQSKIELSTLLILRESINYL"
     gene            17986..19407
                     /locus_tag="U2K71_000784"
     CDS             17986..19407
                     /locus_tag="U2K71_000784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate reductase"
                     /protein_id="HEM7314890.1"
                     /translation="MKKLTKELVKKNEAPVKVLQFGEGNFMRGFIDWQIQQLNNQQLF
                     KGNVAIIQPLEQGLGNMMNQQDNLYTVILQGLSNGEIVDTSEIITVVEQMINPYDEWD
                     AYLSLAENDDLAYIVSNTTEAGIQFVETDNLTDTPPQSFPGKLTAFLYRRFKLNKAGF
                     TIIPCELIDRNGERLKEIILRYAEKWDLEPAFVEWLEKENVFCCSLVDRIVPGYPRED
                     AGVFNQRHNYEDQLMVKAEPFMLWVIEGPKELENQLPFKQAGLNVIITDDMTPYRERK
                     VHLLNGPHTAMVPLALLAELETVEEVMKDPLFSAYVDQLFNLELIPMLSLPKDELAIY
                     ADQIKERFLNPFAHHKLEAISLNSVSKFSTRLLPVFKKYIEEQNQVPPLITVSLAALL
                     LMYRGDQVKPHDDEKTISEFTDAWSDNGTAIPRLLQNAALWGEDLSQIPNVTDTVQEI
                     AAKIDQKGVRSVLQEMKGTMVHA"
     gene            19400..20899
                     /locus_tag="U2K71_000785"
     CDS             19400..20899
                     /locus_tag="U2K71_000785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="altronate dehydratase"
                     /protein_id="HEM7314891.1"
                     /translation="MLETLQPTMKLNRRDSVVVALTPIPKQTQLTIDQQTITTLEDIP
                     QGHKIALGDLKAGDNVIKYGYPIGHVTTDVTAGQWLHTHNVKTNLSGELDYRYEKDVH
                     PSHYLFENRTFQGYLRKNGKVGIRNDLFLVPTVGCVNGIAELIVKQFKEKHPDLGQFD
                     HITILKHPYGCSQLGKDHQNTREILADAVNHPNAGGVLVFGLGCENNTVPEFKKILGE
                     YDEERVKFLVAQDVYDEIEQGVALLEELLTAAENDQRISVPLSKLNVGLKCGGSDGLS
                     GITANPLLGAFSDYLIAQGGSTILTEVPEMFGAEQVLMARAENEEVFDSIVDLINDFK
                     QYFLSYGEPVYENPSPGNKAGGITTLEDKSLGCTQKSGSSPVVDVLQYGEKIRKPGLS
                     LLQAPGNDLVAASALASSDCQLVLFTTGRGTPFGSYVPTVKVSTNTTLFERKGHWMDF
                     NAGVLLEQPMEVILESFVQKIIAVASGEETKNEQNDVREIAIFKNGVTL"
     gene            20938..22332
                     /gene="uxaC"
                     /locus_tag="U2K71_000786"
     CDS             20938..22332
                     /gene="uxaC"
                     /locus_tag="U2K71_000786"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HEM7314892.1"
                     /translation="MFLSDDFLLKDDWAKKLYHSYAKNMPIIDYHCHLDPKEIYENNN
                     FSNLTEAWLGGDHYKWRLMRACGVPEEKITGNASDFEKFLAWCQTVPKIIGNPLYSWS
                     HLELKRFFGIDLLINEENAEKIWTQANEILAAPDFRRREIAKKSNVEVICTTDDPADD
                     LHYHQLLAHEEPDLKVLPSFRPDKALNIEKEGFNAWVTLLEEAADQTITTYQELIEVL
                     GQRIEYFHQHGCRVSDHALDILRYKEADETVLEEIFQKARANKLLSSDEIDAYRTETL
                     IHLTHFYHSHNWTMQLHIHAYRNCNTQMLEKLGPDTGYDGMNDLSLTIPLQKLLNRAE
                     ETDQLPKTILYSLNPNDYPAILALMGCFQKEQNGKLQLGSGWWYNDTRAGMRDQLTQF
                     ADGSALGNFVGMLTDSRSFLSYTRHEYFRRVLCEFLGEMVMRGEAPEDEQLLGSLVQA
                     ISYTNAKNYFGFFA"
     gene            22491..23174
                     /locus_tag="U2K71_000787"
     CDS             22491..23174
                     /locus_tag="U2K71_000787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhamnogalacturonan acetylesterase"
                     /protein_id="HEM7314893.1"
                     /translation="MPTIHIAGDSTAAEKDENKRPETGWGEKISAYFSPEIQINNQAK
                     NGRSTKSFIQEGRLARLASQFQPNDFLFVQFGHNDQKIQEERGTLPFGSYLKNLQIFF
                     ECAKKANVQMVLLTSVTRRDYLENGTLNPDILGDYPKAMRAFAEKHHIPLLDVFSRSQ
                     ELFQTFSKEETKKFYLHLIPDTCKNYPEGLKDNTHFSPEGADKVARIIVELIKESRLP
                     LANYLQKGV"
     gene            23176..24012
                     /locus_tag="U2K71_000788"
     CDS             23176..24012
                     /locus_tag="U2K71_000788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HEM7314894.1"
                     /translation="MFPLNLGIRAHDLDVRNREEIVQKVSQLHLSHIQFAPQKAFPEQ
                     LKLTDMTLGSAAYFGEYFQKNNIELSILGCYINLSSKNQEVRAQALATFKQQIALCSA
                     YQAKMIATETGSVSVGYTKENFTEEAYQIARNSIIELVAHAENFGVTVAVEAGINHPI
                     YNYQLAKRLIDEVASPNLKIILDCANLMHKENHADQEKVIRGALDNLHGYITALHLKD
                     YIMENGKIRMVPVGKGCLDFRPVLHYIKYERPLMYATLEATPEIYVPEAIEHLQFIYQ
                     DC"
     gene            24023..25777
                     /locus_tag="U2K71_000789"
     CDS             24023..25777
                     /locus_tag="U2K71_000789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314895.1"
                     /translation="MEERIQKSYQKYHANAFQTLLHLYQGNYWRFAMSGLFFIIKHLP
                     SWLMPIAIANVINAASSKDSSQLQAIYFNAFLMACLIGQNILTNYFHVKYHSTSIRQV
                     EAGLRATLIKKIQELSIPYQKELQSGRIQSKIIRDVEAIQTLSSQVFVSGLNIAVNLF
                     VALGITLYKSPVVFMFFLLTVPLAAFVTLFFRKRIRQSNSDFRQEMEETSAKVIEVIE
                     LAPIARAHGLEKHETTRLNKHFTQIYEKGFHLDILQSVFGSVSWASFQIFQLICLIFT
                     GILALQGRIQPGDVVMYQTYFTTIVNSVSGMITLIPTISKGMESITSIGELLIAGDVE
                     TYHGKKKIQHLDGNFRFEDVSFLYPDSLKPIIDHFSLEVKAGETIAFVGPSGSGKSTL
                     LNLLIGFIQPTSGKIFLDDQPLDELDMRSVRNYLAVVPQTTLLFSASIKENITYGLKN
                     VSKERLDEVIEAAQLSSLIDQLPDGLDTLVGEHGNKLSGGQKQRISIARALIRQPKII
                     LLDEATSALDNQSEKKIQQALNYLTETPTTFIVAHRLSTIKEADKIVVIDEGKIVEIG
                     TYEKLLAQKGYFYRLKNE"
     gene            complement(26053..26394)
                     /locus_tag="U2K71_000790"
     CDS             complement(26053..26394)
                     /locus_tag="U2K71_000790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEM7314896.1"
                     /translation="MSNIYDSANQIEREIRELPEFKALKEAYEKVQANEEAHKLFKDF
                     QAMQIELQEKQMSGQEFSDEDAMKAQEMAVKIQSEEVINDLMQKEQGFSTIINDLNRI
                     IMTPVRDLYSE"
     gene            complement(26462..28633)
                     /locus_tag="U2K71_000791"
     CDS             complement(26462..28633)
                     /locus_tag="U2K71_000791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEM7314897.1"
                     /translation="MDFQSILEKIKAALISFWRWIRPYLGQFHRWRKRIWKKYHVNKL
                     ILLIGLICVLVTSIYLFILAKQANVETLKSGLSQSTVIYDKKNEEAGTLYAQKGTYVE
                     LNAISPYIQDAVISTEDRNFYEHHGFDIKGIARAAVRMVLSGGTSGGGGSTITQQLAK
                     NAYLTLDQTFDRKAKELFLAIEIEKKYSKEEILTMYLNNAYFGNGVWGVQDASRKYFG
                     VDATNVTLSEAATLAGMLKGPGIYNPIDHPKNANDRKNTVLSVMEENGKITKEQEQAE
                     SQTDISAYLNDTYDNSDSGYRYPYYFDAVIDEAVNEYGLDEEDVMNKGYKIYTALDQD
                     YQKQLEATYQNSALFPANASDGAMVQSASVALDPNTGGVQALVGRRGDHVFRGFSFAT
                     QMRRSPGSTIKPLSVYTPALEAGYKPSSILKDEPQSYYDAHNFDGTYQGEVPMYQAVA
                     QSLNLPTVWLLHEIGLQKGYDKAEEFGLPLAKSDKYYGLALGGLEKGVSPLIMAGAYS
                     AFANDGQMYTPHLITKIVDSTGAVIVDNTNKKPKQVISKETADEMTSMLLGTFSNGTA
                     AAANPAGYTIAGKTGTTETNFDATKANDQWMIGYTPDVVISTWMGYEESSKLHYLEGT
                     SGNVVGKVFKSAAEGILPYTSKTGFSVADAYATGGQVVAADQVGNTGNSNGESGNWQD
                     NLNQYGEQAKEGLKNFGDMVKEGVQGIGEAAKDLWRKYQGE"
     gene            28802..29701
                     /locus_tag="U2K71_000792"
     CDS             28802..29701
                     /locus_tag="U2K71_000792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM7314898.1"
                     /translation="MVQLVCDCTERSTLVKITITLPMNHPETTIRDLLEKEWLVPRKV
                     RHFLRIRKNVWINQQPALFHHEVKGNDQITLLFEETDYNYQEVQLGDAKHIRVLYEDE
                     HLIIVDKPAGMKTHPNEPTENSTLLNHLAAYLDTKQQRPYVVHRLDKETSGAVLFAKN
                     PFVLPILGRMLEQKMIYRRYQAIVWGEIAKDMTITNKIGRDRHDRRKRVVDDKNGQSA
                     LTHVTVNKVLSEQTQVYCVLETGRTHQIRVHLAHKGHPIVGDPLYQNRTAERLMLHAL
                     ELHMIHPFTQEKIVGKALPGLWE"
     gene            complement(29772..30629)
                     /locus_tag="U2K71_000793"
     CDS             complement(29772..30629)
                     /locus_tag="U2K71_000793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM7314899.1"
                     /translation="MYGGIEAGGTKFVCAVSDGGEILEKLSIPTTTPEETLDQVFEFF
                     DRYELKGIGIGSFGPIGIDKTKDNYGYVLATPKKGWAQFDFLGTIKERYNIPAAWTTD
                     VNAAAYGELKQGAAQGKNSCIYLTVGTGIGAGVVINEEIFSGIAHPEMGHIWVKRHPD
                     DHYEGTCPYHKDCLEGLAAGPSIEARTGIKGQDLPQDHPVWAIQAYYIAQALINYTLT
                     LAPEKIILGGGVMNQDHLLQKIRQQFVELMGGYMETPQVSEYIVRWGLPNESGIIGSL
                     LLAEHEHQK"
     gene            complement(30614..33316)
                     /locus_tag="U2K71_000794"
     CDS             complement(30614..33316)
                     /locus_tag="U2K71_000794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-mannosidase"
                     /protein_id="HEM7314900.1"
                     /translation="MKKKVYIISHSHWDREWYMAYEQHHMRLIELIDDLLELFEVDPS
                     FNSFHLDGQTIILDDYLQVRPEKRQAIQQAINEGKLRIGPFYILQDDFLISSESNVRN
                     MLIGMEESRKWGTPVMLGYFPDTFGNMGQTPQLMKQAGISAAAFGRGVKPIGFDNQVL
                     EAENYSSQYSEMWWKGPDQTAIFGLLFANWYSNGNEIPVEKEAALAFWKQKLADAEQY
                     ASTNHLLMMNGVDHQPVQKDISKAIHLANELFPDYEFIHSNFTDYLEAVQKDVPEDLG
                     SVEGELTSQETDGWYTLANTASARVYLKQWNTKVQRQLENITEPLATMAYEVSGNYPH
                     DQLDYAWKTLMQNHPHDSICGCSVDSVHREMIPRFEKADEVGKYLAQDSLEQLTAAID
                     TTGFPKDSFPFVIVNTAGMDKTGEAEITIELERKRFAEGIPEQLYQELENLPKRKYHV
                     ETKSGATIPAILSEETVQFGYDLPKDRFRVPYMARMIKVTLPLENMPAFSWETFALVE
                     GEAKAEEKETMIHQSGRIIENGPLHLTIEKNGTITMEDRKNKRKLNNLHIFEDIGDIG
                     NEYIFKQPFCDQPILSSNKENSEVKVLVDTPEIAKISILQEMEIPVSADERLEKEQQM
                     VVEFRYRKAERSKEKRILQIKTIMTIRKDSKKIDFETTLDNQMKDHRLRVLFPTKLHV
                     EHHEADSIFEVVKRPNHVSKSWENPTNPQHQQAFVNIHDEEYGVTVGNFGLNEYEVTE
                     DGQIAVTLLRSVGELGDWGYFPTPEAQCLGEHRFNYSIELHGPEEKFSTYLHAYAAQI
                     PFSTQQIKHHEGTLISKQQYLTIKSETFAITALKRSKFSDKVVVRGFNMSSHLEKLEI
                     TKDNGKTVILNLLEEPTKQAVVPIIQPYEIRTIGFEEENECMAE"
     gene            complement(33329..34618)
                     /locus_tag="U2K71_000795"
     CDS             complement(33329..34618)
                     /locus_tag="U2K71_000795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 125 protein"
                     /protein_id="HEM7314901.1"
                     /translation="MAYKDIPKSVQKFMEDITQKCGDIHADWAKNFTAAFANTLLTTV
                     KKQNDGTTFLLTGDIPAMWLRDSTAQVRPYLVIAKEDEELADMISGLVKKQFFYINID
                     PYANAFNEEANGAGHQTDHTKMNDWIWERKYEIDSLCYPIQLAYLLYKNTGKTDQFNQ
                     SFIEGIKKILDVFKTEQDHEHSPYTFVRDTTRKEDTLINDGRGAKTAFTGMTWSGFRP
                     SDDTCRYGYLVPSNMFAVVVLGYVEEIFQTILDDKEIVQQTQKLKKEIQEGIQNHGRT
                     VNQKGQTIYAYEVDGMGNATVMDDSNVPNLIAAPYLGYCSEQEEDYLQTRRTLLSKEN
                     PYFYEGKYAKGIGSSHTPEKYVWPIALAMEGMTTSDKSEKERILNLLVATDAGTHLMH
                     EGFDVNNPENYTREWFSWANMMFCELVMDYFDIRVEK"
     gene            34768..35814
                     /locus_tag="U2K71_000796"
     CDS             34768..35814
                     /locus_tag="U2K71_000796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7314902.1"
                     /translation="MNQPLYQTIFNDLQTAILTGELPVDSQLPTEKELSRTYKVSRIT
                     SKRALTELEQLGLIYRVRGKGSFVKSASPEQILASPTKTRRILFLLPYVADLSVGDFS
                     KGLNPVMQDKQIDVMMTTLDFLQNKTAKELINEFDGLIYYALDTEQHLDLLFELSLKE
                     FPVVILDKKIYELPFPTVLSDNFQGGALAASYLIEQGHSKIAYLFGTQTHPQSVRQRY
                     LGYLEALDKAGLTFHTSLDDKSATLDELLSYITKYEVTAFVCENDVVAIQTMNLLRQH
                     GYHIPEDFSIIGFDDIQAAALVDPPLTTIAQDFEQLGKLAGDALVTWLETKDRPSDSK
                     FPVSFIKRQSTKEK"
     gene            35816..37969
                     /locus_tag="U2K71_000797"
     CDS             35816..37969
                     /locus_tag="U2K71_000797"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GH92 family glycosyl hydrolase"
                     /protein_id="HEM7314903.1"
                     /translation="MNIGQIDTRHATANQPNFSNGNCQPYTGVPFGMNYFAPQTTDQN
                     GSWWFHPDDRVFQGYRLTHQPSPWMGDFSHMLFTPINGKLQENTLFHAQSSYRPEESI
                     FCPTHLSICQLRYGICSTLIPSMYGGILSIDYSKNDSGLLLSFPGRHQLFVIDPYTVE
                     GKITHFAGSEDPDFSFYFILRFEYPLTIDELAVSNKNSDSIPLYFEQTKKQTIRFGTS
                     FISSEQAAYNLELEQNQSKETYLAHSQEQWQSYFDRIQLTHHDQQQLRTFYHNLYRVF
                     LFPQTFYEIDPSGQKIHYDTTSRSIKPGVLYTNNGFWDTYKTVYPLYSLIAVEKYEEM
                     LEGFLNSYRESGYLPKWLSPDERGLMPGTLIDAVIADAAVKDIRRDLMPEFLTAMEKG
                     ATVQSKHPNYGRQGTEDYLTYGYVPNHYHESVNHTLDYAYSDFCISQVAKVLEEEEMT
                     KKYQQQSLNYQNIFDYESGFMRSKDKDGKFREPFSANRWGQDYAEGSAWQSSFAVYHD
                     FSGLIGKYGGSERYKEKLIELCNQPPTFDPGGYGFEIHEMSEMAAIEFGQLAISNQPS
                     FHYPYLFSYIGQPEMAQPLLKQLMTQTFDDSPMGYPGDEDNGSMAAWYIFNSLGFYPV
                     TPGTGEYVIGMPLIDKAVLLLSNGKELTIEATPNRPQQQFIHKIKRMDKEYTKLYFTH
                     QDLLKGGTISYQLGIIPHPKKYQAKDLPFSLSNYN"
     gene            complement(38442..39893)
                     /locus_tag="U2K71_000798"
     CDS             complement(38442..39893)
                     /locus_tag="U2K71_000798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7314904.1"
                     /translation="MSVYQVLLVDDEYMILNGLKKIIDWQSLGFQIVATAENAKEGLA
                     VLEQRQIDLVVTDVTMPEINGLEFIEAAQKERHNFEFMILSGYQEFDYLKGGMQLGAV
                     NYLMKPVNKFELVESLKKIKTRLDQQNEQKNQQEIYQELLFSQWLNEELDEISEEEII
                     GQISEKQHRIVLIQLARTHDTLINRWLQERQQIFYYQRNYGDRVLITLLLEADEVDGF
                     CCFVQENFPDQEWLISIGEETDSLDKIPETFQQAKDNLQLHQFYGDKDEHVFYAVQAE
                     TKDQTIDFSSFRRVLQNKQLEVAQKMITDFFEQFQLAAMMPEDIRYSSFLLFMEIQRE
                     LIDLEDEEYLQGIEKIHQAKTVQELQQLLLSFIKKHQRQKKYSDNVEKVIVILHQHYQ
                     EPLTLKEVSENLHLNVMYLGQLFKKETKKSFSAYLNHLRMEKAKQLLLHSNQNINEIA
                     SEIGYNNTTYFSKLFKKIVGRSPKEYRENSGES"
     gene            complement(39890..41614)
                     /locus_tag="U2K71_000799"
     CDS             complement(39890..41614)
                     /locus_tag="U2K71_000799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM7314905.1"
                     /translation="MNKFRILFSKGTLLNRLLRKYAVIMIGVTMTATVIFSIHTWEQN
                     QKQAENMTSDAVQSTSRMLNDKTTLSRIIKNQLVGNSEKIENVTTYLTKPIDQYLMYV
                     YEQQNSTDELVSFPNQIKDLYANYEELSAIYIVLNQLPEYYESSRENKGGEIKKGTPK
                     LLDAFYIKLPITQGGAESGSIFIGFQKKELDMILENLTSFNGLSLYMISGTTNRLYTF
                     HDNKIAKETFERQEMIITESLKKNSQLPIDDLEKNNFVQHQELSDDYRILAVLDKKSV
                     KIETGKNLAPLIIGIIILDNILLIFLYKTFKRYSQQVSIVMQAMDQTAAGNLETRIDI
                     TNTEYELKELSIGINEMLDSINQFVEDIYKLEIKQQDAHMRALQAQINPHFLYNTLEY
                     IRMYAISEGSEELADVVYAFSALLRNNTNQEKTITLKEELDFCEKYVYLYQMRYPNRV
                     AYHFMIDPDLEKIEVPKFVIQPLVENYFKHGIDFTRFDNALSVKVLQEGKRVRIIIKD
                     NGKGMTEKRLKQIEEKLSHPKVELHGSIGLQNVNERLRASFGSSYYMSLENNETGGLT
                     VSITFKEG"
     gene            complement(41607..42221)
                     /locus_tag="U2K71_000800"
     CDS             complement(41607..42221)
                     /locus_tag="U2K71_000800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF624 domain-containing protein"
                     /protein_id="HEM7314906.1"
                     /translation="MLGKVLEELFIRIWVIIKLTLYFWIYTFAGGIIFGLGAAWKTVN
                     ELFYLYGFEYKEITIKRGWNIYKRNFLRGNLLFSLFLSGTALLSYNLYLSVQIKGLIF
                     LAIDFILLFALLCLFATYQYSMILDSEYTISVPNLLKLSFISVFSSFSSFLKIIIGSG
                     IILGVTWQFKGLILFGVIGLLTVWNGTMTTHWREELDKQLESYE"
     gene            complement(42566..44023)
                     /locus_tag="U2K71_000801"
     CDS             complement(42566..44023)
                     /locus_tag="U2K71_000801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM7314907.1"
                     /translation="MKLWKKLAFTGVSALLLGTLAACGGSGSKDQAEASNSDTLQMYQ
                     IGDKPDNFDQLMEVANKRIEKEIGVKVNINYIGWGDYEKKMNVIISSGENYDIAFANN
                     YVSNAQKGAFADLTELAPKYAEKAYNDLDEAYIKGNLVNGKLYAFPVNGNVFAQQVLT
                     FNKALLDKYDLSIDGIQSYADAEKVLKEFHEKEPNTAAFAIGQGFKVQGNFDYPLGNT
                     LPFAIDLDGDDSKIINQYDNETYIGLLRTMHSWYKQGLIPSDAATSNKDYPLEGNTWL
                     MRGETQGPYDYGDTILTNAAKQELVSKAITVPLKSTAQAQMANFVVSNTSKNKEKSVE
                     LLGLLNSDPELLNGLVWGIEGEAWEKVDGSDDKIKLLDGYQPNTHMSAWNTGNNKILY
                     TQESITDDMIAKRDQSIADAETSPILGFSFNTDSVKTELSNISNVMNQYLDGLNTGTV
                     DPDETLPKLKDALDKAGYDKVLKEMQKQYDEFRQE"
     gene            complement(44064..44984)
                     /locus_tag="U2K71_000802"
     CDS             complement(44064..44984)
                     /locus_tag="U2K71_000802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM7314908.1"
                     /translation="MKKKKVERVQVRSFNKTANFFSNIMIALFAISCILPFIFVIAIS
                     FTDESILSTHGYSFWPEKFSIFGYTYLFDQMQDKIFQALFVTVLVTVLGTLINSTATS
                     LYAYAISRSNFPFRRFFTVFCLITMLFSPGMVANYLVMTNLLQLKDTIWALILPMAVS
                     PFNIIVMRTFFKRSVSDSIIESARIDGASELRIFVQIVLPLAVPGIATISLFAALGYW
                     NDWFNALLYIQDDKLVPLQYLLMKIQSNIQYLTQNSGAGSQLTGGLASVPGESARMAI
                     VVISTLPIAISYPFFQKHFVKGLTIGGVKE"
     gene            complement(44996..45943)
                     /locus_tag="U2K71_000803"
     CDS             complement(44996..45943)
                     /locus_tag="U2K71_000803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7314909.1"
                     /translation="MKKRKGFNHFFMNLWRYKALVLMAVPGMLWMIFFFYIPVMANVV
                     AFKNFHISADGFLASLRESPWVGWENFKFLFSSDQAFLITKNTILYNVAFILLNLLIS
                     VVFAIIMSELRNKRLVKVYQTMSLLPYFLSWVIISYFVYAFLSPDKGIFNQWITGHGG
                     EAVNWYNEPKYWPFILVFIGTWKGIGYNSIIYFASVMGIDPTYYEAAMVDGASKWQQI
                     KHVTIPQLIPLMTILTILAVGNIFRADFGLFYNIPRNSGALYDVTQVLDTYIYNGLTS
                     TGDFGMTAAAGLYQSVVGFVLLMITNTIARRFDSDSALF"
     gene            complement(46422..47141)
                     /locus_tag="U2K71_000804"
     CDS             complement(46422..47141)
                     /locus_tag="U2K71_000804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA pseudouridine synthase"
                     /protein_id="HEM7314910.1"
                     /translation="MRLDKFLADMGLGSRKEVKGLIKKGTIEVNGQVVKSDKAQVNEF
                     EDAVTYLGEIITYQKDFYYILHKPAGVISATQDNHDQTVIDLLEDQDYREDLFPVGRL
                     DKDTEGLLILTNDGVFAHQLLSPKKHVEKEYLAEVSGIMTEEDIRLFAEGLIIDGDEQ
                     TLPAKLQIDQVDQETETSIIRLILHEGKFHQVKRMVKAVGKEVLYLKRIRMGKFWLPK
                     DLNKGQYRPMTKEELEQVREK"
     gene            complement(47190..48836)
                     /locus_tag="U2K71_000805"
     CDS             complement(47190..48836)
                     /locus_tag="U2K71_000805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM7314911.1"
                     /translation="MDNQRKPALDQLTPQEKMARGSAWMTASNIISRLLGAVYIIPWY
                     AWMGENAKAANGLFNMGYNIYALFLLISTAGIPAAIAKQTARYNSLNEYGTSRRLFIR
                     ALQMMGGLGLLFALFMYIASPWLAHASGGGEELIPTMRSLSIAVLVFPCMSVIRGYLQ
                     GNQEMMPYALSQIVEQVARIFYMLLATFIIMKVMDGDYVTAVTQSTFAAFIGMLASIL
                     VLLYFLKKHQAYTSAFIHYSENKINIATKELLLDTVKEAIPFIIVGSGVTIFKLVDQF
                     TFMKIMSASTEYSNAQLLDLFSIFSANPDKLTMVVIALATSIASTGLPLITEAVTLKD
                     RVGLAKLTSGNLQLFSFFMFPATFGVMLLAYPLNTLFYTPDSLGSNVLIQASFVGLFL
                     GLYMLVSNMLQGMFENKAAINYLVIGFLVKLLLQYPAIRLFEVYGPLLATMIGFAVSC
                     TLILKRIHEVARFKPSFVWRRTLLIFILTLIMLLASWITKMIFGAFLNEDSKFQSLLL
                     ILVVAGIGALVYGYLALKIRLAERLLGPGIARLRHKLKIK"
     gene            49015..49605
                     /locus_tag="U2K71_000806"
     CDS             49015..49605
                     /locus_tag="U2K71_000806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM7314912.1"
                     /translation="MTQEIKKLPVIFLGLFFITVGLNWFLLPHDIASAGVGAIGHLVE
                     TAFSIQRSVTVWSINLMMLLLAAVLLGKSVFFKTVAGSLLFPVVLEIVPKVEMISSHF
                     FSLISGSLLFSLGVYTLYTVGASNGGITIPPIIFHKFFRLPMPQGLLLTNSLIVFLNY
                     YVFGLLETLFVLLSITVSSLFMKILIRLSPVSRKFE"
     gene            49697..51202
                     /locus_tag="U2K71_000807"
     CDS             49697..51202
                     /locus_tag="U2K71_000807"
                     /EC_number="6.3.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--L-
                     lysine ligase"
                     /protein_id="HEM7314913.1"
                     /translation="MSFSLEDIRHLLLKENLLKEFVSTRGWHLDVPEKAEFLQLSYDS
                     RKADASTLFFCKGMNFKEEYLDSAIEQGIQYYVSEQPYENSAVGIIVTDIRKAMALLA
                     MAFYDYPQNKLKVIGFTGTKGKTTAAYFTKAILDHTTNKKTALLSTMNTTLDGKTYFK
                     SHLTTPESLDLYRMMSEAVENGMTHLVMEVSSQAYKTQRVYGLTLDVGIFLNISPDHI
                     SPIEHPTFDDYFYCKRQLILNSKTVVLNHESDYFDLLRETADLFQIPTITYGRSEESN
                     YQVIRSDKGTHGFTLSSHEDRLAICDTAYDILLAGGFNQENAASAIIAAALTGASSQD
                     AQDGLKEARVPGRMDQLIQSNGAHVYVDYAHNYLSLKTLLKFAKNEHPDGRVIVVLGS
                     PGNKAISRRHDFGKVLSETADIAFLTADDPAFEDPKKIAEEINEAITNPDLIVHYEMD
                     RPEAIRRALAESAPQDSVVIAGKGVDPYQKINGVDEPYEGDYAIVKRLIEE"
     gene            complement(51286..52638)
                     /gene="celB"
                     /locus_tag="U2K71_000808"
     CDS             complement(51286..52638)
                     /gene="celB"
                     /locus_tag="U2K71_000808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEM7314914.1"
                     /translation="MFDFLQKYLMGPMGKIAQLKIVRAVMAAGMASIPFTIVGSMFLV
                     LNILPLPFPFLEGFFNATFFKVSDLYMIVNTMTMGILSIYFAIVFAYELTSIERDEQK
                     LNVNPLTGALLSVFAFFMCIPELIMTDGKISLIASINETETVVNGIRMGSFVERLGTS
                     GIFTAIIMSIIAVELYCMCVKRNWVIKMPDVVPPGVSRSFTALIPTFVIAFVVMIING
                     LLVALGTDIFKMIAIPFGFVTQLTNTWLGVMVIYFLIHALWIVGIHGANIITSFLTPI
                     VLANMAANAQGANYPLAGEFNNSYVTVGGSGATLGLIIFIAFLAKSDQLKVLGKASLV
                     PGLFNINEPIIFGMPIVYNPYLAVPFFLAPMASATLAYFAIRLEIVKPMLAQMPWPSP
                     VGIGAFVGSGGDWKAAVLAVICAIIAFIIWFPFIKFYDNKLLNEEREKAAELASEKLK
                     "
     gene            complement(52638..53156)
                     /locus_tag="U2K71_000809"
     CDS             complement(52638..53156)
                     /locus_tag="U2K71_000809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314915.1"
                     /translation="MQDTVKRKIELEKKQLSLKNMYFNRYLIVRYLTAFFFFMNMQWM
                     ILLLSAKSLGSSLPMILILAILPAVGEQVKLYRKHQTNVPWTKWYFLFQSVFNLLVIP
                     VLFTSGFTFLYPFMANNNGGQLFVFILVVSGIFASAFIQYRLKKISLNQDQQYIRIKQ
                     YEKALYLGKENN"
     gene            complement(53172..53498)
                     /locus_tag="U2K71_000810"
     CDS             complement(53172..53498)
                     /locus_tag="U2K71_000810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIA"
                     /protein_id="HEM7314916.1"
                     /translation="MAEKINSETLQVTSFDIIFHSGNARTLIHEAFRSMRKEMFEEVE
                     EKLRQANEEILHAHQSQTQLLKEYASGTKIEMEIIMVHAQDHLMTTMTLLEVAQEMNY
                     LYQKNK"
     gene            complement(53511..55490)
                     /locus_tag="U2K71_000811"
     CDS             complement(53511..55490)
                     /locus_tag="U2K71_000811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM7314917.1"
                     /translation="MKQKEKLLLRYLIDHKKEYVTSQRLASELLLSDRTIRNYLQRIK
                     ELVEKNGGKIIAKPGYGYQLHILQRLTFDLFLSRQEIVPGYAREPQGFYESEDRQKYI
                     LNQLLLEDAILFMDDLAEELYISRSSLTKDMQEIKERLIPYSLKIVSKHGQGTWIEGE
                     ERNRRHFIMDTFFGNHYGNSLKEYLGQSRFFPDISFEELVIIILDETREAKLKVSDFI
                     IQNLTLHLALGIKRLREGFEIKELGIEQEVSKRVEYQVAQRIVQRIEVIAGVSFPEEE
                     IAYLTLHLMAKSNHKENQNDQELSKELAKVLKRLSQEFHFSLVEDLQLFNGLLDHLSP
                     MMIRLSRGIRIENPLTEEIKKENPKVFDAVKRHFSNMPALKNYTINEDEWAYLALHLM
                     AALEKERAAHKLHALIICATGYGSAQLLKNRVVSEFGKNITVVSVKGYYEINETTLKG
                     IDLIISSIDLSTMFFKIPVLHVSIFLNEDDVRKIRKVVGESIPYHSSDRPIPAFSLQQ
                     KKQIYTEQLSEKFFKTYTYEPKKEEILHDLLQKLSINEEEGYIREMRKQIQQRENMGQ
                     IIFSDTVAVPHPAVPVGIETKVAVALIPSGMQWDQYQEIHFVFLVSPSYVENEGITVV
                     TKAIVRLVDQLKVQQEILAEPIFENFSSKFIKLIE"
     gene            complement(55511..55825)
                     /locus_tag="U2K71_000812"
     CDS             complement(55511..55825)
                     /locus_tag="U2K71_000812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIB"
                     /protein_id="HEM7314918.1"
                     /translation="MKKALIICAAGMSSSMMASKTTDFFKQKREEVLVDAVSATEGDN
                     MIKNSDFDLFLISPQTTMFLDKFKKLGESVGKPVVSIPFQAYVPIPTGIQKLAEVIEE
                     NI"
     gene            55993..56286
                     /locus_tag="U2K71_000813"
     CDS             55993..56286
                     /locus_tag="U2K71_000813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HEM7314919.1"
                     /translation="MSITVNILYTSTNGQARKFAEEMVERNIVKAIRAEEGNEKYDYY
                     FPLEDPESLLLIDRWKDEEAIEKHHKSEMMAQINELRKKYKLKMKVERFTDLP"
     gene            complement(56364..57476)
                     /locus_tag="U2K71_000814"
     CDS             complement(56364..57476)
                     /locus_tag="U2K71_000814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM7314920.1"
                     /translation="MTVKNKSKKKGRQQVDFYQSLQLDPFILKQKIREAASKKEKCMY
                     ICSLFLRSLFIVLFAICFIIIITTLFESKHKPYAVVLFCMLMSIRFVDFGYKISHSII
                     GLAIVMFSLLVAPYVQLIKWSVMGMLIHFILLSSILMATASDPKMGNASLYGFSYLFI
                     VYSLPKDSLNKNFFTQTSSLLFLFFCWFSVLLYRKHREKNKDKSLFKEIFLKGMYSQE
                     KIWMLIYAFGISLLIVAGEYVPFQRLMWAGFAFSSIVSSYGLMSIGFKERAVDRIIGS
                     LIGCVLFIGISQFIPFNWVGILGGLALGVCSTYRYKTVFNSFGALAITASLFGVPGAV
                     TIRIFENILGVCLGIMYIGVTEILIRKIREKHGLNH"
     gene            57863..58042
                     /locus_tag="U2K71_000815"
     CDS             57863..58042
                     /locus_tag="U2K71_000815"
                     /inference="COORDINATES: protein motif:HMM:NF026041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314921.1"
                     /translation="MPMVIRLKKQRYTCKNCRSHWNAQSYFIRPRHSISNHVRHKITS
                     LLTEKVSLFFISKSC"
     gene            58236..58406
                     /locus_tag="U2K71_000816"
     CDS             58236..58406
                     /locus_tag="U2K71_000816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016615560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM7314922.1"
                     /translation="MPRLTNYFLSCTNPKEIEFLVTDMNATYFQLTKHVLPNAKLVID
                     RLLGFPTPFKEA"
     gene            complement(58830..>59263)
                     /locus_tag="U2K71_000817"
     CDS             complement(58830..>59263)
                     /locus_tag="U2K71_000817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM7314923.1"
                     /translation="GVVLTKIDDQSGEVLQGAVFELQDANGTVLQSGLTTGADGKLAI
                     DGLAPGAYQLVETQAPTGYELDATPVTFKIEKEQEVAIFLTKENRKVADSSDIRGNNP
                     TSSGNKHLPKTGETIISQFILSILGVLLVFISIKFRKKRNI"
BASE COUNT        17755 a        12079 c        10353 g        19076 t
ORIGIN      
        1 ttgtcttgaa ttgaatcaag acgccctaca catttggttt gtcttacttt gttcagtttt
       61 caaaggtcta cttcatcgag agtgtttccc gattcatttt tggctgccgt agcaactttt
      121 atatcatatc agagagacaa acgattgtca actctttttt aaaagttttt ttcgatttcg
      181 tttttcgaaa tctttcagca actcatttat cataacttat cgtttgttat ttgtcaagaa
      241 cttttttgaa gtttttttcg atagaatcaa gcttcgcaag tggttctttt taacgactta
      301 gttatcttat cacgtctaaa aacatgtgtc aactcttttt tggaattttc ataaaatgtt
      361 tcaatgaagc gcgacaacgt tgtttaatat atcattaaaa aaaataaagg tcaagctaaa
      421 atattattta agcttgacct tattataatt tcaacgattg tcaaaagtat tgtttgcagc
      481 tgtgttgcca gtttctatag ataaatcaca gcttttgaaa cataagtttt gtactgcttc
      541 gattcgcccg aattcttttg cagttatttg gcaatgagaa aagcagacat cttctatagg
      601 cttttcagga aatgctacta aatcaaaagc tcgggaagga agtgagtaac ctggagaaag
      661 gcatgcctgt acgtttttga tgtgtatgtc tttgactttc gtcatttgct ttgattcagg
      721 tatttcttca gccactgctt gccaactttc tggcatttga gcaaggtcat tgctattttt
      781 tctattgtat tcattatgcc agtccattat ccaagaaaaa ggaaattgta catcaatcat
      841 attcagattt tctaccctga tattttcgat cactccccct ctttcttttg aagatttcat
      901 tcgaatacca cagtctgact gaaaaaaatc gatatcgtga atatatacgt cactaactcc
      961 agcactaact tcgctaccaa tagttacacc gtatcctgaa cggattttac atctagcaac
     1021 ctcgattctt gatgattttc gattgactct tgcaccattg ccatctctgc cagacttaat
     1081 agcaatacaa tcatcaccac aggccaaatc acattcatag acacgcacat ttgttgaaga
     1141 atcaatatca atcccatctg tacttggtcc atcattgttg cggatgatta ctttttctac
     1201 taatacatcg ttagaataag tgatttgcaa gttccaaaaa cctgatttct gaaaaatcaa
     1261 atcacgaaca acaacatgtt ctgcatgata cagtaaacaa gcgcgtggac gtttaatagc
     1321 ataatccgca atccaacgaa gtcccttttg atcgtattca gctcttgtcc cgcttttttg
     1381 atcttttccc caatacagct cccaccaatg gtctccttga ccgtcgatta ttccttttcc
     1441 ttctataaga atatctttcg ccgagagaac attcaaaatc gctgctggcc atttcatttc
     1501 tacgccggct actcgtgtat cgatttctgg aaatgcttcg atatctgttc tccccttgag
     1561 gactgcacct tcttcaaaga taagatgact cttatctttt aaaaaaaggg ctccgactaa
     1621 ataaatgcct tttggaataa atacaggtaa gtgttctttc gcaccttgat cgattgcttt
     1681 ttgcagtatt attgtatcat cttgttgagt gtcgtttgtt ttaaagttca aagcattgat
     1741 aatccctttt tttgtccgca atacgtttct ccctatcttt cattgatttt tttcaattct
     1801 tcaccaataa atacagtttt tgaacgattc agtgggtaac ctactagcaa aatgattgcg
     1861 gatacaaata agcagaatgc tggtaacaag tttgccatcg tataaatcct ttcttgtact
     1921 aaaactgatt ggatggctcc acctgttgta gacgattggt aaccaatgag tgccagcata
     1981 aatccgccta ccgctcctgc acatgcttgt cctacttttc tcgcaaaaga atttactcca
     2041 tatactgttc catcttctct taagcctgtc ttatattgat gataatcaat cacgtcagta
     2101 atgaatgccc agaccatcaa attgaataaa ccagatccta aagtagcgat aaataaaaga
     2161 atcagatata cccatgcatt tgttatatga aggaaataca tcagcaagta catacatgaa
     2221 gaagcaagta acgcaaatac gcttgcttcc tttttaccaa ataattttgt cagctttgta
     2281 gcaaatggtg ctaaaactac tactgttccg taagtaaata aaagagcaac agacattgct
     2341 tgtttattat ggaagtaatc attgaataaa taagtcatat tcgtcccagc taaaatctga
     2401 ttgatcacaa tgaaaatgtc aacaatgact aaaataatca gtgctcgatt agctaacaaa
     2461 ccttttacca actgagaagc agatactttt tcgtttttga caactcttac tctttcagta
     2521 gtcaatttac aagttaacat atatgcgaca aatgctattg cagcacatcc gattgaaatc
     2581 caaaagaaat gattccctga aacgacttgt tgtccactct tgttatgggt atacattaaa
     2641 atcggaatga agaatcctgt agttgatccg ccaatcgcag aaccaatgct tcggaacgtg
     2701 gacaacgagg tgcggtcatt aggatttgag ctaattgctg ctgccattgc tccatacggg
     2761 atattgattg ttgagagaaa aatcccataa acaatgtatg aaacaaaaat ataagcaatc
     2821 ttcaccgtca ttggaagatg ttggacgaat ggcaaaaaca aaatgacagt cactaagcaa
     2881 aatggatact tcatttttct gatccaagga ataaatcggc cagtttctgt taactctgag
     2941 acatcacata atcttccaac catcacatca gcaaacgcat ccaaaattct cgaaatcaag
     3001 aataacgtac ctacgattgc ccctgaaacg ccaagaacat tcgtataata aatcatcaag
     3061 aaactattga ctaaacctaa aataaaacag ttacctagat caccgaacat gtagcctatc
     3121 ttatcggaaa tcccaaattc ctttttttct ttttttattg tatttgctgt aacagcattt
     3181 tgtgaaagca tttgaattcc tcctacctct tctagtaaac gttttcaaaa ttataactct
     3241 ttttttcttg tttttatatt cacaatgttg gctttttatt gcattatctt gacagataaa
     3301 aaacaaaaaa taatctaaaa acattgatgc ataagattga taactttttt tacataacaa
     3361 tatatacaaa caaaaaaatt cactaactag tgaaaataag ttagtgaacc tcatttatta
     3421 aagaattaaa ttacagatga tcttttccat catacttgaa aaactcaaaa tcagcataac
     3481 ttcctctttt tttatttaga tcatgcactg ctatcccgat aaaatttcca gtaaaccctc
     3541 ctgagagtat acacatgttt ttttgcggac ctactttctt ccattcttca gttgatacgt
     3601 ctttcaaatg aaactgtgct gtctctttct ttacagtgac tttcaaaaaa gtcttctttt
     3661 ctgtgagtgg aatcgtccaa tcttctattt gtgctactcc gttttctgat tctatcatac
     3721 gtaggcattt tcccttgtcc tcattccaag taacataaca ataaatataa ttggattcat
     3781 ttaagtacag taccagtcca gccatctgat taaaattatt aggcatgtat tcaaatgctg
     3841 tctccgcatc aaaagtaaaa tccgtttgcc gaatagctaa caaatgatgt tcgaaggtag
     3901 attgaagaga ttcaccggaa atcagacgta aaaaacctgg tctacatttt gtatcacacc
     3961 aatcagcatt tggcaaaatg cgtctggcat tccagttctt ctttagctct ccttcaaatg
     4021 tatccataaa ctgatgatct ttttcttgtt gtacctcttc atttgtctca atcattattt
     4081 cttccatcgg agttgtctgg ctatctttaa gtcttaacca atcgtcggac gtccaataca
     4141 cttcttgaat agcggtttct cttcccaaaa tagccgcacc gtttaatggt ctggtacaaa
     4201 gatatgtcat gtaccatctt cctgacgggg tttgtactaa gctcccatgt cctgtacatt
     4261 gcaatgtaga agcaggtttg tcactagctg tcagcatagg aaaaccagga tcaagctcgt
     4321 aaggaccagt aatattcttt gctcggcaaa ctgtaactga atgtcctctt cctgtaccgc
     4381 cttctgccgt catcaaataa taatacccgt tacgaaaata caaatgtggt gcctccgttt
     4441 tcgctaattc tgtacctgca aatattttat agatgggacc atcaagcttc ttcttctctg
     4501 catcatattc ttgtaatacg ataccgctgg atttattcgg tgtgtccaga cgataatccc
     4561 atagttcatt gacaagccat ttttttcccg tttcaagatc atggaacaaa gaaggatcaa
     4621 agccgttact atttaaataa attggttcgg accaaggacc tgtgatctca gatgcagtga
     4681 tcaaataatt gtggacatct ttaaacggac ggcctgcact tttgacatct gtgtaaacaa
     4741 gataaaacaa tccatcagca taactgattt gtggcgccca tatacttcca tttgtaggat
     4801 ttccttgcaa agatacttga gtggtcaaaa tatccgttgt atgtgaccag ttgactaaat
     4861 ttttcgactc atatactcgg attcctggaa gccattcaaa agaggagaca ataatgtaat
     4921 accgctcttt cactcttaat atattaggat ctggattgaa tcctcttaaa ataggatttt
     4981 tcactttgat agtctgcatg attcaggctc cttttctcat tcttactcga tataaactga
     5041 atgccccagc tgcgaaataa aaaaatgcaa aaggaaacgc aataaactga gtgccaatca
     5101 acaatatgaa tcctgttata aaatacaaac cagtggcagt tgttaagtat tttcctttag
     5161 cagtcaaaaa caatgctact gctaaaaaag caagtgaaat aaacaacaca acggcaaaca
     5221 gaccagtcat gtcctgtaat aatccaaatg tattttctaa agacaaagtt gaatgatcta
     5281 cagcaagcga ctgataactt tttttaaact ctgacgcaga catattactt aaagtaactg
     5341 ctaacccgcc aaagaaaatc aaagatagac tgccaccgac cattcctagc aatagttccc
     5401 attttctttt catttctgaa ccccctgctt ttctaaatgt atccattcag gtcttttgat
     5461 cgatgtttca taattgttat tacgttcaga gaatttgaca gtctgttcat ttttcttgtc
     5521 atcccaatta tcccaaccag ttgggtgaac atgcccacct atcttacacg cttcaaataa
     5581 gacatttgca tacggacgcc acggtcttcc taaatagacg tttctagtat caggttctgc
     5641 agtcaaggta cagctttcaa aacgataccc tgttttttga tttttcggcg tagatgctgc
     5701 tgtgatgtat ccaggatttt tactggctaa gctatggata tgacactgat gaaaaacggc
     5761 atctgcccca ccaaatatga aatccaccgt tccttcaatc cagcaatatt cataggtctg
     5821 acgacaatac tcaggattgg cagaagccgg tgtacaaaaa ggagtaccat ctttttgcaa
     5881 atcaggcaac ggacccgtgc ataaggtatc ctgataacca gatagtcgac agttttttaa
     5941 agatacgcga tctcctgaat tgaataaagc aacagcctgt cctacctttt ctcctggacc
     6001 tgctgtattt tcgatgtgaa gattttctaa tacgacattc gatccttcta agaaaaaagt
     6061 agcacttcgg aaagtcccta actggctacc gtcaagatac tcctgttttg caaaacgatt
     6121 accggagata actactgttc ctgctcccac aagtttaaaa ttggataatc tgctttctat
     6181 atattcatca tatcttcctt cttttactat caagaccttt ggatggctat ccggcaggcc
     6241 tgccaaataa tcgattcctt cttggatagt ttcaaaatcc gtcggggtat ctatagcatt
     6301 ctttccaatt atcagtttgt ctttcatcct cataaatgct cccttatgaa ttgtttccaa
     6361 atatttctca tttcttgtgt ttccatatga ccgcctgtta aagaaaaaga agaaaagttt
     6421 tctggactac ccattgattg atactttttg tccagccctt ttctcagctt cagtacgcct
     6481 tcgtccaagc acatcgtatc gttttttcca actaagctaa gacgagggcg cggagcgatt
     6541 ttttcttgga ttgctaacgt tgtataagct gtcaatagat ttggcacata gtaataaaac
     6601 ccatgatgat ctaggcggcg atgtagcaaa agtatttcga tgtccacctg tgccgctaga
     6661 tcaatacaaa ctttgacacg agaatccaat gctgtcaccc accagcttaa taagcctccc
     6721 atagaaagac caatcgttgc gagccgttga gtatctgtgt cttcccttgt ctccaaataa
     6781 tcgatcaagc tgatcacatc ataaatacgc attccccata acgtttttcc tgttaataaa
     6841 aattgtttga acaactcgct ttcagaaata cctccgcgtt cttcaaatcc ccaagcatca
     6901 atcgaccaaa cagcatatcc cattcctgtc agtacttctg caaaagatgg agaagctaag
     6961 taaggagcac catggatcaa ttcacttttc ccgactgaaa aatctccgcc gtgagaatgc
     7021 aggtaaagga ccggcggaaa cggtccttct ttttctggat aagcatagag ggctggtact
     7081 gtttccaatt catttaagta caattgatag tattcaagca taaaaccgtt ttgtttttct
     7141 cggtataatc gttttccact aggggtatgt ctgctagatg gagcacctaa aaaatcattc
     7201 aactgcataa gcaaagtttt tacgaacaac cagttcatcc atattttctg attgatttga
     7261 ttggaatttc tcaatactga tttctttagt attttctaat agaaatgctg gtccctcgtg
     7321 attttcaatc gacatctggt taaaggaaac cttacccgca aagccaatat aaaatccttg
     7381 tctgctcatt tcctctattc ctgtcatcat agcaggaaca ccgggaactg catcttgggc
     7441 catcgagaca tcgatattgt caaatgtgat ttcttgtatg aattgttccg ctaatccata
     7501 aataaaacct gcagaagcat ggacattcct tgccgtgata ttcgaaaaat ggatccttct
     7561 gaatgctggt gtccgttcat ctatcggata agcctttttt tcccatacat acggttcttt
     7621 ccctcgcgga ccacagaaat aataaagatt cagtataaat ggacacatca cattatccat
     7681 gactatattg gatactcgaa tatcttcaac gatcccgcct cgacctcgtc tagatttcaa
     7741 tcgtattcct cgatcagttt cttggaagat acaatttgaa atggtaatat tacgaatact
     7801 accactcatt tcacttccta atacgacacc gccatgtcca tggaccatcg tgcaatttgt
     7861 gatcgtgata ttttcgcaag cgatcctttc gtacgtatct tcagtccctg ccttgattgc
     7921 aatacagtca tctcccacgt caatatggca attactaata cgcacatttt tgcatgattc
     7981 tggatcaatt ccgtctgtat ttggtgaatc cgctgggttc aagatcgtca agttatcaaa
     8041 cgttgcatta ctgcaaagaa tcgggttgat cgtccaactt ggtgactgga tcaatttaat
     8101 atctttcaca gtaattcgat gacagttatg gaaactcatc aatttaggac gaggataagc
     8161 gagattatct ggttcatttc taaaagtatg ccaccatttt tttccatttc catctaatgt
     8221 accgaaacca gtgacagaga tgttctctac attttgagca tagatacagg aagcataaac
     8281 ttttcgatgg actccttccc aacgtgaatg aacaacagga taatcttttg gatcgtcaga
     8341 gaatttcaga accgctcctg cgcttaaatg aagttcgaca ttgcttttta aaaataaagc
     8401 tcctgttaga aattcacctg ctggaacaac tacggtcccg cctccgtcac ttgctgcagc
     8461 atcaatggct tgttgtattg cttcagtatt caattcatca attgatgcgc caaatgtcaa
     8521 aatatcatac atatttataa tgcctccact tcaatcaatg cttgcaagaa tgcaccaacg
     8581 cctttttgat cattacaaat aattggttca ctgatgtagt aagcatatga accatctcgc
     8641 tgatctgctc cgcctaaccc tgctacttga cagtttttat tcaagttcag ccagccttct
     8701 tttgtttcca gaacaaattc atcaagtaat ccttgatgtg atttcttcac ttgtggaagc
     8761 cattcttctt tgttcagcag tcccaaacgt attcctttag ccattgcgta aacaaacata
     8821 ctactgccgg aagcttccag ataatttcct ttccgttttg tttctgttgt tacttgatac
     8881 catgtcccgc tgttttcttc ttgatatgga agtaatgcag ccagtgtctt ttctagaatt
     8941 tctttcagca aagaacaatc tgtttcttca tgaagtaatt cataggtatc tgctgcagcc
     9001 atcaaatacc agccaatcga tcggctccaa aaatgatggg atagacctgt ttttggatca
     9061 gcccatggtt ggaccttctt ttcatcccat gcatgaatca gcaaatttgt ttttggatcg
     9121 aatagatgtt tataactgat ttcaaactgt ctcaaaacat cttggtaatc tttttcttca
     9181 ctaaaattct ggataaattc agcataaaaa ggagaaccca tatataagcc atccaaccaa
     9241 atttgataag gataaatttc tttatgccaa aaagctcctt cggatgtgcg tggatggttt
     9301 gccaactgct gggaaaggag ttctgctgct tttttatatt tttcttcgcc tgtttctttg
     9361 tacaataaaa agaaaagctt tcctgtattt acatggtcga tattgtattc ttcaacgctg
     9421 tagccacgga tcgttccatc ttcttgcacg aaatagtcga tattgtcttt tatgaaatca
     9481 aaataaattt tttcttgggt tcgttcccaa agtaattgga accctttaag tacgacgcca
     9541 tagtcataag accattttcc tttataccac ttttcttcat ataagcgagg tgttctttcc
     9601 atgatacttt ttgctaattt ctttgaccag ttttccatta tttgctctcc tttaaaaatt
     9661 aaaataggat ttgggacaaa taccaagaag tatttgtccc aatcgtctat attactgtgc
     9721 cgctgctttg tatgcttcct cgtactcttg tgtgattcga tcgcctcctt gtgctttcca
     9781 gtcttctaca gcttttttga atccatcttc gtcaagctgt cccatgatgt attgcaccgt
     9841 agcatcaatg acgatttttt caatttctga tcctacttca ttgttcgtcg gtgaatccaa
     9901 tggcacagaa ggatccatta cagcaaattc ttcattttct ttgatcaatt gattagagaa
     9961 ttctttttca gggtctgtat ctttgaaaga gaacgtcact tcactaggac gagagcttga
    10021 taacggttgc acctcttgtt gccaaagatc catatcgata aattcgatag aaccatcatc
    10081 attcaattca taatgcgttc cttcgatacc gccagtcatc aatttataca catcttcttc
    10141 taaaagatca tcaacaaatt gaagtaattt cttcaattgt gcttcgtctt ttacttgaga
    10201 tttgggaaat gctagcaagc cgccaatacc atttccttct gaccaaacat gataaccgcc
    10261 gccttcacca tctgagatct tgtttacagg cactaattcc atatcatctt gaataccttg
    10321 acccatcgtc ttcagattct tgatatccac cattccagtg tagattccag ttcttccttg
    10381 tgcaaattgt tcttgctgat cagtcttttg ggtaactgca aagtcttgtg ccaaatagcc
    10441 attctcaaat agatctcttg aaaaattcaa agctttcata tactcttctg tatcaaattc
    10501 tggagtgaat ttgccattat cgtctacagc ccagccattt ggtgctccaa aataagaagt
    10561 gaaaagttta aagctggtat aacgaatatc actaggcgca cgatcgccaa aaccaactgt
    10621 gtcgtccaca ccatttccat caggatcgtt ttctgtaaat tgtttagcta cttcataaag
    10681 ctcatccatg gtagttggaa catctagtcc tagattatcc aaccagtctt ttcgaatgac
    10741 taagccagca cgagcgtagt ctttttgtat cggtacgccg tacaaggcac cgtcgatact
    10801 cgcagcttcg attctatctt cagaaatttg acttaaattt ttatagtctt ttaaatattc
    10861 tgatacatcc cagaatacgc ctgattttaa cgcattgcgg acagaagagt ttttcaacat
    10921 cgcaatttga agtgtaacga tatctcctag ttcatcagaa gccaatgcag ttgttaatct
    10981 ttcttctttt gaagcatcag gaatccagtt gaatgtcagt tccgtatcgg ttttttcttc
    11041 gatcaagttc acaatcgtgt ctgtaggcgg tgaagcatga tgcaaaatat ttaaccaagt
    11101 gattttagct tttccatctt ctgtctctga agaactgtta cttgaactgc tgccgcatgc
    11161 ggcaagtgct agaattcctg caaaactaag acctaatttt acgagcgttg cttttttcat
    11221 aaattttctc cttcactgtt ttaataatat ttttgttttc ttctgttttt tctgtatatg
    11281 agttcttcca ttccaaatgg caaaatatag aatttgctgt ccacgtgact gcggaaaaga
    11341 agagactgcc accgataaag gttaaaacac ctggcattgt ttggatgatc atcagataaa
    11401 tccctaccat acttaatatc aacactaatg agtaatgaag atgtgagaat ccgaataaaa
    11461 atgccatttt cattttgaaa aaatatcctt tccaatcata atgagccatg attgggaaaa
    11521 tatagagtgc tgagagcaca tatatgaacg cagcaatata caaagcgatc ctaagatacc
    11581 aattgtgtat gaaagaaaga tccacaagta aaacaatccc gatggcgcca tatatccagc
    11641 tcatcagcaa ggtttcacga tagttttctt taaaatatgt gaagtacgtt ttcgtcaaag
    11701 gaaacgtttc ttttgagcgc agccattttc taatcactgc aaccatcgca tacgatgcag
    11761 gaccgaaagt aaaaactccc aaacctgcga tgactaaacc gccccacaga aaattcaaat
    11821 aaaccaactg taaaatacga tttagccttc cgtttatttt atctaatgac tgcagcatat
    11881 ctttccctct ctttctatct tagtaaacgc cgtcttctcc tagctttttg gcaatcttat
    11941 tagctaaaat taccataaac aatcctacga cccctttaaa caatcccatt gctgtactga
    12001 aacttaactg tccattcttc aatccagctg tataaatata ggtgtcgaaa atttctgcaa
    12061 cgcttcgatt caatgaattc aataacaagt acatatgctc gaaccctaag tctaatgtct
    12121 gaccgatttt tagaatcagc aaggtaatga ttactggacg aattgccggc aatgtaacat
    12181 gccatgtttt gcgtaagcgt cctgctccat ccatttctgc agcttcatac aactgtgtat
    12241 cgactgcagt tatcgctgaa aggtagatga tcgtagacca gcccaactct ttccagatca
    12301 cttgtccgat atatactgtg cgtgtccatt caggggatgt tagaaaactg attttttcaa
    12361 atcccattgt tgcaaggaat tcattcaata cccctccgtc aacattcaaa aatacataag
    12421 taatcgaaac aatgattacc caagacatga agtgagggat gtaaatgatc gtttgaatcc
    12481 cgtttttcaa tcgtttgttc gttacttcat ttaatagtaa agacaaaatg attggcatcg
    12541 gaaaaaacac gaagatattt aatccgaaca agatcaacgt atttcttaat aatgtaaaga
    12601 atgtcggttc tgtaaacaaa cgaatgaaat gtttgaaacc gacccaagga cttcccatta
    12661 ttcctaaaaa tggttgataa tcttgaaaag caataacgag tccccccata ggaagatacc
    12721 taaaaatcaa gaaataaagg actcccggca gaatcatcac atataaaaat ttattcgttt
    12781 tgattcctgc aattattttt tttcgtcttc tgagcttttc ttcttttttc gatagcgtca
    12841 gttgaaatgg ttcttgtttc tttacacttt ccataagccc ctccttagtt ttgtgaatgt
    12901 ttgctgctta tgacttcatg atagataact atctgaatta cgtatataat catttttacc
    12961 gtaagtaaaa acgcttacat agcaatgatt tttgtgctat acaaaacgat aaagtgtatt
    13021 ttttcacaaa taaactacgc atagcaaacc ttctattttt ttctatctga cacttagcat
    13081 aggaaatgat tatatacgta atctttcacc gatgataaat tgaacatata gaaacagaaa
    13141 catttaattt attttgagga ggagcctatc tatatgaaaa agaaagtctc tataggagaa
    13201 agaagtttta cacttttcaa caatacgttt ctaattttgc tagctttgat ctgtatcgtt
    13261 ccttttttaa atatcatcgc tacatctttt gcttctacac aagaagtcgt cgcgaaaaag
    13321 tttatccttt ttcctactac cttttcttta gacgcgtatc gctacattct ttctacgccg
    13381 accattttta gagcacttgc tgtttcgatt ggtgttacag gtgtcggaac gatcgtcagt
    13441 atgtgtgcaa cttcactgat ggcctacggt ctttccagaa agtatttgtt tggtcgaggt
    13501 ttcgtcaact ttcttgttgt attttctatg ttgttcagcg gggggatgat tccaacgttc
    13561 ttagttgttc gttctctagg gctggtcaat tcttactggg ccatgatcct gcctgtggct
    13621 gttaatgcaa tgaatatgat cattatgaga aactttttcc aagccttgcc ggatagtttg
    13681 gaagaatcag caaaaatgga tggttgcaca gattttggtg tatttttcaa aattatgctg
    13741 ccattagcgt taccgtctat tgctactatc tcgctttttt atgctgttac ttactggaac
    13801 acttatatga ctgctatttt gtatatcaac gattcatcta aatggccgat acagattctt
    13861 ttacgccaaa tcgtcattgt atcaagtggg atgcaagcag aaagtagtgc cgttgatgtg
    13921 attccaccag cacaaacagt taaaatggct gttatcgtta ttgcaacagt gcctatgctg
    13981 attgtttatc cttttgttca gaaatatttt gtcaaaggag cactcgtagg ttctgtcaaa
    14041 gggtgattat gtactgaacg caaaatggta gttgtgtcag aagtggtcag cttcaagaaa
    14101 taagaaggca tttacgaaaa ttgcttctta aatttttgtg aaatttcggc ttatttttcg
    14161 aagaatctac tcctggaaca ccgtgtatca gaggttgtga aagagacgtt cagccttgag
    14221 cgatgataaa aactctcaca aacattgcga cgagtaaccc acaggagcaa taagaagcga
    14281 ttttgaaaaa tagcttccca tatttcacaa aattatgcct tattgtcgat agcctgtctc
    14341 ttgaacgcca tttattcgta aaaaagaact gtgacaagac ttttgtcaca gttctttttg
    14401 cgtgcttagc cttgaaattt aactatccat aaatacaaat actagggtta ttctaacttc
    14461 ttttattgct tgaatcagtc cacaagaaaa acagcctctt gattcacaaa tatttttttc
    14521 gatattctcc cggcgtaatc gattccttct ttttaaagaa acgaataaaa ttttgcggat
    14581 ttcgatattg caatctttct gcaatctctt ttacggagag gttagtctct ttcaaccatt
    14641 tttttgctac ctctaaacga tgattttgaa caaaatcacc aaatttttct cctgtttcct
    14701 ttttaaatac attacttaaa tagttagggt tataatgcaa cctatcagcg atcacatcta
    14761 aagacagatc acgatcatat tctgtgtaga cgatatgcat aatattctcc gacaaatttt
    14821 taaattcatg atcagtgatt gcttgagagc attctgtgat tggcagaact aattgttcta
    14881 aaacaagttt ttctagttct ttgggatgat aattgttcag tgcttttaca taaatttgtt
    14941 tcatgttatc aaagtttttt gaatctactc ccaataactg tcctaattga acgagttcat
    15001 tgatcaagcg gaccgaaact acttcccgac tcaaaggatt tttattttgt gcaaacaatg
    15061 catcaatcaa ttcatgtact aaattcttca cttcctgatt tccagaacga atggcttcaa
    15121 acaatcgatt ttgttcttct atcggatatc taataagtgt tttttcatga gcagcaggaa
    15181 cgatttcctg ataaaaaata attgaacttg gaccagtatt tacacgataa tacagggctt
    15241 cttttgcccg atcaaccgat tgcttgcttt cattcagcgt ttcgaaccgg ttactgatgc
    15301 ctatactgat agttaaattc aagtattctt ttacacgttt ttgaatgagt cggcaatact
    15361 ccatgacttt tttgcttgct tctgcatcat ctttatccat tcgataaata gtggcttgca
    15421 tttcttcatt caaaacgatc ggcagcattc gattttcctt tggcacaatt tccgtgatca
    15481 tgttgttgat cgctaaaagt agcaaatcac gctccccacc gtgactatct tctaacagat
    15541 cgatttgaac caaagctgta taatagcagt cattttgaga actataacca aattgctgca
    15601 accgctgatc tagttctcgc ctttctactc gatttctaaa taaactaagt acaaatagcg
    15661 tttccagctg tggcttttgg gtaactaagt gtgcacttaa cgcttctttt tctcctatga
    15721 tccgactgac tgattgagcg accaaatcta gttctttccc actgaatcct tttgatttaa
    15781 gattcagacg ttcctgtatt tgagaaatcg gacgggcaaa tctgtccgaa aataagtagg
    15841 aaagaagacc aatgagtcct accagtgtta aagacactgc aactagcccg attctcagat
    15901 tgcggatcat tgcgccaatt gcactagagt caacttctaa tctgtaaatc caattattat
    15961 aatctgattt cagtactata tacgttttat ctttttctct tatcactttc agctgattgt
    16021 aatctagatc cacgtccgtt aatacagttg gttgctcttt attttttact gtctgagtag
    16081 taaaaaggtt gccaacttcg ttttcaatag aaagtatgtc ttctccactt tcaccaacaa
    16141 cttgttctat tgtatgtttc tctatcgtag caattcccaa tgcatatttt tctttttcaa
    16201 acattggcaa agaaatcacc atttcgattc ctttgttttt cttttgccaa aataaattat
    16261 ttttgttatt tatgtacttc tgttgatact tttcaacttc ctcttcggtc agttgactca
    16321 acgatccatt gattactccc catttaccag ccaaactgac taaatcataa ttattatcat
    16381 ccataacaat cgtttctata aaatttagtt ctttcctgat ttcagaataa gtggagtaat
    16441 ctgtatatgt cagcggatgc ttgaatatgt ccgagaaatt tttggtcgaa ccatacgtag
    16501 aaaacgaata ttcaatcgtt ctaattttcc attcaacgtt agttttaact tgcctgagca
    16561 aattcttttt gtctgttaaa taactcgatt cgatcgattc ataggtattc tggtaaatga
    16621 ataaactaaa tcctatgaca agtacggctc ctaataaaaa catcggtaaa aatattttat
    16681 aaaatactgt acttttcttc attttcttcc tctcctcctt ttaaaataaa aggctttcat
    16741 ttttctgtaa aattgatact catctaatta aatgagcatt ccccgctttc aagaatagca
    16801 taattaaaaa taaaaatcca agtaattttt aacgtgtgca atcttttcat atcaaagatc
    16861 tattgactta tcgtagttat cgtaatatga tgaatgcgtt aacaaaaagt gaaaaagtaa
    16921 ggaaaatgat ggaatggtaa cgatcaaaga tatcgctaaa gcagctggcg tttctcatac
    16981 gacagtttct cgtgctttga ataacagcag tttaataaaa aatgaaacaa aacaaaaaat
    17041 acgacaacta gctaaggaaa tgaattattc gccaaactat agtgcaaaag gtttagtgaa
    17101 tcaaaaaaaa tatctaatcg gactattttt ctcaagtttg cataaaggaa cttcatcaaa
    17161 tttcctagca gattccattg caggcatcca ttcagtatta gacacaaatt acagtttatc
    17221 tgttgaaagt atcgatgttg ttgatttaac agaaatcaac ctgcaacgct acgatggact
    17281 gatcgttatg agtcaaagtg atgatgatca gcctttcatc gactatctca aagaaaaaga
    17341 atttcctttc gttgttgtca atcgtcacat caatgatcca gcggttatca acgtcactgc
    17401 cgatgatgcg gccggtgtta tgcaggcagt agattacggt attggatgcg gacatacgaa
    17461 gatcgcttat attggcggaa agaaaaattt tcgttcgaca aaagaacggg aaaaaggggt
    17521 attgaaaagt ctaaaaaaag cgagcatccc tgtcaatcca gcctattttt taacaggaga
    17581 ttacagctta gaaagcggcc tccaaaacat ggaataccta ttatcacttt ctgacctgcc
    17641 tactctggtt ttttgcggca acgacgacat agcgattggt gcattgagag ctgtacattt
    17701 agctggcctc cgtgttcccc agaacatttc tctgatcggg ttcgatgatt ctccagtcgt
    17761 ctcctatctc aatccgccgc ttaccaccgt acgtaagcct ttgaaagaaa tgtgtcaaga
    17821 aggaacaaag cttttactag atttgatcga aggcaggacg atcgaacaat ctaaaattga
    17881 gctctccacc ttgttgatcc ttcgtgagtc gatcaattac ttgtaaacta caagtaattt
    17941 tatataaaaa tgttaacgtg tgcataacac agaaaggaag tttatatgaa aaaactgaca
    18001 aaagaattag tgaaaaaaaa cgaagcacct gtaaaagttc ttcagtttgg tgaaggcaat
    18061 tttatgcgtg gatttattga ttggcagatt cagcagctga ataaccaaca attgtttaaa
    18121 ggtaatgttg caatcattca acctttagaa caaggtcttg gaaatatgat gaaccagcaa
    18181 gataatttat atacggtcat tttacaagga ctcagcaatg gcgaaatcgt tgatacttcc
    18241 gagattatca ccgtagtaga acaaatgatc aatccttatg atgaatggga tgcttatcta
    18301 tctctagcag aaaatgacga cttggcctat atcgtctcca atacaacaga ggcaggaatc
    18361 caattcgtgg agactgataa tttaacagat actcccccac agagcttccc gggcaaactg
    18421 acagcctttt tataccgacg tttcaaatta aacaaagctg gctttaccat cattccttgc
    18481 gaactgattg acagaaacgg cgaacgtcta aaagaaatca ttttacgcta cgcagaaaaa
    18541 tgggatttag aacctgcttt tgttgagtgg ttagaaaaag aaaatgtttt ctgttgtagt
    18601 ttagttgatc gcatcgtccc aggttatcct cgcgaagatg caggtgtttt caatcaacgg
    18661 cataactatg aagatcaatt gatggtcaaa gcagaaccat tcatgctttg ggtgatcgaa
    18721 gggcctaaag aattggagaa tcaactacca ttcaaacagg ctggcttgaa tgtcatcatc
    18781 acagatgata tgactcctta tcgtgaacgt aaggtgcatt tgcttaatgg accgcataca
    18841 gcgatggtcc ctcttgcttt gttggcggaa ttggaaactg tagaagaagt aatgaaagat
    18901 cctttattct ccgcatatgt tgatcagtta ttcaatctag aattgattcc tatgctttct
    18961 ttacctaaag atgaattagc catttatgct gaccaaatca aagaacgttt cttgaatcct
    19021 tttgcccatc acaaattaga agcgatttca ttaaatagtg tttcaaagtt cagcacccgt
    19081 ctgcttccag tgttcaagaa gtatatcgaa gaacaaaatc aggtaccacc gttaatcact
    19141 gtttcactag ctgcgctact tcttatgtat cgaggcgatc aagtaaagcc acatgatgat
    19201 gaaaagacga taagcgaatt tactgatgct tggtctgata atggaacagc tattcctcga
    19261 ttattgcaaa acgctgcgct ttggggagaa gatttgtctc aaataccgaa tgtgacagat
    19321 acagttcaag aaatagctgc aaagatcgat caaaaaggtg tccggtcagt attacaagaa
    19381 atgaaaggaa cgatggtcca tgcttgaaac cctgcaacca acaatgaaat taaatcgacg
    19441 agacagtgtg gtcgtcgcat taacccctat tccaaaacaa acacaactga cgattgatca
    19501 gcaaacgatc accactcttg aagatatccc gcaaggtcat aagattgcat tgggtgattt
    19561 gaaagctgga gacaacgtga tcaaatatgg ctatccaatc ggtcatgtga cgacagacgt
    19621 aactgccggt caatggctgc acactcataa tgtaaaaacc aatctttctg gtgaattgga
    19681 ttatcgatat gaaaaagatg tccatccaag tcattacctt tttgagaacc gaacatttca
    19741 aggttacttg cgaaaaaatg gaaaagtagg gattcgtaat gatttgtttt tagtacctac
    19801 cgttgggtgt gtgaatggca ttgctgaact gatcgtcaaa cagttcaagg aaaaacatcc
    19861 agatctggga cagtttgacc atattacgat tttaaagcat ccttatggct gttcgcagct
    19921 tggaaaagat catcaaaata ctcgagaaat tttagcggac gcagtaaatc atcccaatgc
    19981 cggaggagtt ttagtatttg gattaggttg tgagaacaat acagttcctg aattcaaaaa
    20041 aattttaggt gaatatgatg aagaacgagt gaaatttcta gtggctcaag atgtttacga
    20101 tgagatcgaa caaggtgtgg ctctattaga agagctttta acagcggcgg aaaatgacca
    20161 gcgcatatca gtcccgctct ctaagttgaa tgtcggtttg aaatgcggcg gttctgatgg
    20221 tctatcggga ataactgcta atcctttact tggtgcattt tctgactact tgattgctca
    20281 aggaggcagc acgatcttaa cagaagtgcc agaaatgttt ggcgcagaac aagtattgat
    20341 ggcacgagcg gaaaatgaag aagtttttga ctcaatcgtt gacttgatca atgacttcaa
    20401 acagtatttc ttgtcttacg gggaaccagt ttacgaaaac ccatctcctg gtaataaagc
    20461 aggtgggatc actacattag aagataagtc tttaggctgc acgcaaaaat caggaagctc
    20521 tcctgtcgtt gatgtgctac aatatggcga aaaaatcaga aaaccagggc ttagtttatt
    20581 gcaagctccc gggaatgatc ttgtagctgc ctctgctctt gcttcatccg attgccagtt
    20641 ggtcttgttt accacaggaa gagggactcc gtttggctca tatgtcccaa cggtcaaggt
    20701 atctacgaat acaactcttt ttgaacgcaa aggtcattgg atggatttta atgcaggcgt
    20761 cttattagaa caaccaatgg aagtaatctt agaatcgttt gttcaaaaaa tcatcgctgt
    20821 agctagtgga gaagaaacga aaaatgaaca aaatgacgtt cgagaaattg ccatttttaa
    20881 aaacggagta acactttgat tttcataaaa cagtataaaa agaaagaagg aagatttatg
    20941 tttttatctg atgatttttt attaaaagac gattgggcga aaaagcttta ccattcctac
    21001 gcaaaaaaca tgcccattat tgattatcac tgtcacttag atccaaaaga aatttacgaa
    21061 aacaacaatt tcagcaatct gacagaagct tggttaggag gcgatcatta caaatggcgc
    21121 ttgatgcgag cttgcggcgt gcctgaagaa aaaatcacag gaaatgcttc tgattttgaa
    21181 aaatttttag cttggtgtca aaccgtacca aaaattatcg gaaatccgct ttacagttgg
    21241 tcacatcttg aattgaagcg tttcttcggt attgacttgt tgataaatga agaaaatgca
    21301 gagaaaattt ggacacaagc aaatgaaatt ttggcagctc ctgattttcg ccgtcgggaa
    21361 atcgcaaaaa aatcgaatgt agaagtgatt tgtacaacag atgatcccgc agacgacttg
    21421 cactatcatc agttgcttgc tcatgaagaa ccagatttga aggttctacc ttcattccgt
    21481 ccagataaag cactcaatat tgaaaaagaa ggatttaatg catgggtcac actactggaa
    21541 gaagcagctg accaaacgat caccacctat caagaactga tagaagtctt agggcagcgg
    21601 atagaatact tccatcaaca tggatgtcgt gtttccgacc acgctttaga catcttacgc
    21661 tataaagaag cggatgaaac agtattagaa gagatttttc aaaaagcacg agctaacaaa
    21721 ctattatctt cagatgaaat cgatgcttat cgtacagaaa cactgataca tttgacccac
    21781 ttctatcatt ctcataattg gacgatgcag ctgcacatcc atgcttatcg taactgtaat
    21841 acacaaatgt tggaaaaatt agggcccgat accggctatg atggaatgaa tgatctttct
    21901 ttgactattc ctctgcaaaa attattgaac cgtgcagaag aaacggatca gttgccaaag
    21961 acgatccttt attcattgaa tccgaatgac tatcctgcga ttttagcttt gatgggctgt
    22021 ttccaaaaag aacagaatgg aaaactgcag ttaggttctg gatggtggta caacgatact
    22081 cgagctggta tgcgcgatca attgacacaa tttgctgatg gtagtgcttt ggggaatttt
    22141 gtcggcatgc tgacagattc aagaagtttc ctgtcctaca ctagacatga atatttccgt
    22201 cgtgttcttt gtgaattttt aggcgaaatg gtcatgcgag gagaagcacc tgaagatgaa
    22261 caacttttag gaagtctagt tcaagcgatt agttatacaa atgctaaaaa ttactttggc
    22321 ttttttgcct aattgattaa tagtaacagt aacgaatcaa aaacaggata gtgaaataac
    22381 ggaatccgtt tatccacgtt ttagagaaga agacggacca aatttgctcc gtcttcttct
    22441 cttgctgttc tgcttcattg cctataaacc tttatgaaag aagagataac atgcctacta
    22501 tccatattgc cggtgattct actgcggcag aaaaagatga aaacaagcgt cccgagacag
    22561 gttggggaga aaaaatctct gcttattttt cacccgagat ccagatcaat aaccaagcaa
    22621 agaatggacg cagcaccaaa tcatttatcc aggaaggaag gttagcccgt ctagcttcac
    22681 agtttcagcc gaatgatttc ttatttgtac aatttggaca taacgaccag aaaatccaag
    22741 aagaaagagg tacgcttccc ttcggtagct atttaaaaaa tctgcagata ttttttgaat
    22801 gcgcaaaaaa agcgaacgtc caaatggtct tactgacatc tgtgacgcga cgagattact
    22861 tagaaaacgg aacattgaat ccagatatac ttggagacta tccaaaagcg atgcgtgcat
    22921 ttgctgagaa acaccatatt cctcttttag atgtgttctc aagatcacaa gaattgttcc
    22981 agaccttctc aaaagaagag acaaaaaaat tctacctgca tcttatacct gatacttgta
    23041 aaaattaccc agaaggacta aaagacaata cccactttag tccagaaggc gctgataaag
    23101 ttgcccgaat aattgttgaa ttgataaaag aatcccgtct gcctcttgct aattatttac
    23161 aaaaaggagt ttgagatgtt tccattaaat ttaggcatcc gtgcccacga tttagatgtt
    23221 cgaaatcgtg aagaaatcgt tcaaaaagta tcacagcttc acttgtccca tatccaattt
    23281 gcaccacaaa aagcatttcc tgaacaactt aaattaactg acatgacact tgggagtgct
    23341 gcctattttg gtgaatactt ccaaaaaaac aatatcgaac tttccatttt aggatgctat
    23401 atcaacctat ccagcaaaaa tcaagaagtt cgtgcacaag cattggcaac tttcaaacag
    23461 caaattgctc tgtgctctgc ctatcaagcc aagatgattg ctacggaaac cggtagtgtc
    23521 tctgtcggtt acacaaaaga aaattttact gaagaagctt atcagatcgc cagaaattct
    23581 atcattgaat tagtcgctca tgcagaaaat tttggcgtaa ctgttgctgt tgaagcaggg
    23641 atcaatcatc caatctataa ctatcagtta gctaaacgtt taatcgatga agtagcttca
    23701 ccaaatttaa aaatcatttt agattgtgcg aatttgatgc ataaagaaaa tcatgctgat
    23761 caggaaaaag tgatccgagg tgccttggac aatttacatg gttatattac agcgcttcat
    23821 ctcaaagact atatcatgga aaatggaaag atccgcatgg tacctgttgg caagggctgt
    23881 ttagatttcc gcccggtact gcattatatc aaatatgagc gtcctctaat gtacgccact
    23941 cttgaagcta ctcctgagat ttatgttcct gaagcaatag aacacttgca gtttatttac
    24001 caagattgtt aaggagaaga ttatggagga acgaatacag aaaagttatc aaaaatatca
    24061 tgcaaatgct tttcagacat tgctgcatct ttatcaggga aattattggc gttttgccat
    24121 gtcaggactc ttttttatta tcaaacatct tccatcttgg ttaatgccga tcgctattgc
    24181 caatgtaatc aatgcagctt catcgaaaga ttcttctcag ttgcaggcca tttatttcaa
    24241 cgcttttttg atggcttgtc tgattggtca aaatatattg acgaattatt ttcatgtaaa
    24301 ataccatagc acttcgatcc gtcaagtaga agctggacta agagcgaccc ttatcaagaa
    24361 aatccaagaa ctatctattc cttatcaaaa agaattgcaa tctggacgaa tccagtcaaa
    24421 gatcatccgt gatgtagaag ctatccaaac gttgtcctct caagtatttg tcagcggatt
    24481 aaatatcgct gtcaatttat tcgtagcttt aggtatcacg ctttataaaa gtcctgttgt
    24541 tttcatgttt ttcttattga ctgttcctct cgccgcattc gttactctct ttttccgtaa
    24601 aaggatcaga cagtccaact cagacttcag gcaagaaatg gaagagactt ctgcaaaagt
    24661 gatcgaagtg atcgaattag caccgattgc tcgagctcat gggttagaaa aacacgaaac
    24721 aactcgctta aataagcatt ttactcaaat ctatgaaaaa ggattccact tagatatcct
    24781 gcaatctgtt ttcggatcag tcagctgggc tagttttcag atatttcagc tgatttgttt
    24841 gattttcact gggatattag ctttacaagg acgcatccaa cccggcgatg tcgtgatgta
    24901 tcagacgtat ttcacgacga tcgtcaattc cgtttctgga atgatcacgc tgattcctac
    24961 gatttcaaaa ggcatggaat ctatcacttc tattggcgaa ctgttgattg ctggcgatgt
    25021 agaaacttat cacggtaaga aaaaaatcca acatttggat ggaaattttc gttttgagga
    25081 tgtttctttt ctttatccgg actcactcaa accgattatc gatcattttt ctctagaggt
    25141 gaaagctgga gaaacaattg cttttgttgg gccatctggc tctggaaaat ctacactcct
    25201 taacttattg attggattta tccagccgac ttcaggaaaa atttttctag atgatcagcc
    25261 tttggatgaa ctggatatgc gaagtgtgcg aaattattta gccgttgttc ctcaaaccac
    25321 tttgttgttc tccgcttcga tcaaagagaa catcacttat ggcttgaaaa acgtatcaaa
    25381 agaacgttta gatgaagtca tcgaagctgc gcaactttct tcactgattg accagcttcc
    25441 tgatggtctg gataccttag taggcgaaca tggcaacaag ctgtctggtg gccaaaagca
    25501 acgtatctcc attgcacgag cgttgatacg tcagccaaaa atcattttgc tagatgaagc
    25561 aacctcagct ttagataacc agtcagagaa aaaaatccag caagccctga attacctgac
    25621 agaaacaccc actactttta ttgtggctca tcggctttca acgatcaaag aggcagataa
    25681 aatcgttgtg atcgatgaag gaaaaatcgt tgaaataggt acttacgaaa aactgttagc
    25741 tcaaaaaggc tatttctacc gcttgaaaaa tgaatgacaa aaaataggag ctatgacaag
    25801 acttttgtcg tagctcctat ttctgaataa acggtgttct cctgccaact cctcggtcac
    25861 aagtcacaat agtcatcaaa catgagaagc tgttttttga aattgttcct tgtaactcag
    25921 agttaaacgg taggctcacg acctcttggt aaatggtgtt caagagacag cctatcggca
    25981 ataagaagca attttgtcat cgctcaaggc cgaacgtctc tttcacaccc tctgttgttt
    26041 cataaaatag ttttattcgc tgtaaaggtc gcgaactggt gtcatgatga tacggttcaa
    26101 gtcattgatg attgtactga agccttgctc tttttgcatc aagtcattga ttacttcttc
    26161 tgattggatt ttcacagcca tttcttgtgc tttcattgca tcttcgtcgc tgaactcttg
    26221 tccactcatt tgtttttctt gtaattcgat ttgcatcgct tggaagtctt tgaacagttt
    26281 gtgtgcttct tcgtttgctt gtactttctc gtacgcttct ttcaacgctt tgaattctgg
    26341 taattcacgg atctcacgtt cgatttgatt cgcgctatcg tagatgtttg acatgttatg
    26401 ccctcttttc ttttagtagt tactctctat tgtaacatgg ggttattcgt tttcaaaggg
    26461 gctactctcc ttgatatttc cgccagaggt ctttagccgc ttcaccaatt ccctgaacgc
    26521 cttctttcac catatcgccg aagtttttca gcccttcttt tgcttgttca ccatattgat
    26581 tcaagttatc ttgccaattt ccagactctc cattagaatt tcctgtgttt ccaacttgat
    26641 ctgcagctac cacttgtcct cctgtggcat aggcatctgc tacactaaat cctgtttttg
    26701 aagtatacgg aaggatgcct tctgccgctg atttgaagac tttcccgaca acatttccac
    26761 ttgtcccttc caaatagtgc agcttgctgg attcttcgta acccatccat gtcgaaatca
    26821 ctacgtctgg cgtgtaaccg atcatccatt ggtcatttgc ttttgtggca tcgaaattag
    26881 tttctgtggt tcctgttttc cctgctatcg tataacctgc aggattcgca gcagccgctg
    26941 ttccatttga aaatgtacca agaagcatgc tggtcatttc atcggctgtt tcctttgata
    27001 tgacctgttt tggttttttg tttgtgttat cgacgatcac cgctccagtc gaatcaacga
    27061 ttttcgtgat aagatgcggc gtatacatct gaccatcatt ggcaaatgca ctgtaagcac
    27121 ccgccatgat caatggagat actccttttt ccagtccacc aagggctaag ccataatact
    27181 tatcagattt tgctaaaggc aggccgaatt cttctgcttt atcatatcct ttttgtaaac
    27241 cgatctcatg caaaagccat acagtaggca aattcaaact ttgtgcaaca gcttgataca
    27301 tcggtacttc tccttggtat gtcccgtcaa aattatgcgc atcgtaataa ctttgcggtt
    27361 cgtctttcaa gatactggaa ggcttgtatc cagcttccag tgctggtgta tatacgctta
    27421 atggtttgat cgttgatcct ggtgaacgac gcatctgtgt agcaaagctg aatcctctaa
    27481 agacatgatc tcctcttcgt cctactagtg cttgtacacc acctgtattc ggatctaaag
    27541 ctactgaagc agattggacc atggcaccat ctgaagcatt tgctgggaat aatgcactgt
    27601 tttgataagt agcttctaat tgcttttgat aatcctgatc taaagctgta tagattttat
    27661 agcctttatt catgacatct tcttcatcaa gaccatactc gttaactgct tcatcgatca
    27721 ctgcatcaaa ataataagga taacgataac cagaatctga attatcgtat gtgtcattaa
    27781 gatacgcgct gatatctgtc tggctttctg cttgttcttg ttcttttgtg atttttccgt
    27841 tttcttccat tacacttagt actgtgtttt tccgatcatt cgcatttttt gggtgatcga
    27901 tcggattata aataccagga cctttcagca ttcctgccaa agtagcagcc tcagacagcg
    27961 taacattcgt tgcatctact ccaaaatact ttctggaagc gtcttggaca ccccataccc
    28021 cgttgccaaa ataggcattg ttaagataca tggtcaagat ttcttctttg ctgtacttct
    28081 tttcgatctc gattgccaaa aataattcct tcgcttttcg atcaaacgtt tggtctaatg
    28141 tcagatacgc atttttggca agctgctgag tgatcgtact tcctccgccc cctgatgttc
    28201 cgccagacaa gaccattcgg actgctgcac gggcaatccc tttgatatca aacccgtgat
    28261 gctcgtaaaa attcctatct tctgtcgaga ttactgcatc ttggatatag ggagaaatcg
    28321 cgtttaattc tacatatgtt cctttttggg cgtacagtgt gccagcttct tcgtttttct
    28381 tatcataaat gactgtcgat tggcttaatc ctgattttaa cgtttcgaca tttgcttgtt
    28441 ttgctaaaat aaagaggtag atacttgtca ctaagacaca gatcaaacca atcagcaaaa
    28501 tcaatttgtt cacatgatat tttttccaga tgcgctttcg ccatcgatgg aattgtccga
    28561 gatatggtct gatccaacgc caaaaactga tcaaagcagc ttttatcttt tctaaaatgc
    28621 tttgaaaatc catttgcttc ttctcctgtc tatttgattc aatcgtctta ttttaccata
    28681 gtgaaagagt gttttcttcc tgcttaagtt gtagttcaat atgttttttt acgttagtgc
    28741 aagtagagta acataagaat gaacagaaaa atgtaagttt tttcttaaat atgttttaaa
    28801 aatggtacaa ttagtctgcg attgcactga aaggagtaca cttgtgaaaa tcacgatcac
    28861 tttacctatg aaccatcctg aaacaacaat tcgcgacttg cttgaaaagg aatggttagt
    28921 tcctagaaag gttcgacact ttttgcgtat acgtaaaaac gtttggatca atcaacaacc
    28981 tgctttgttc catcatgaag taaagggcaa tgaccagatc acattgttat tcgaagaaac
    29041 agattataat tatcaagaag ttcaattagg agatgcgaag cacatccgtg ttttatatga
    29101 agatgagcat ttgattatag tggataagcc tgcaggaatg aagactcatc caaatgaacc
    29161 gacagaaaac agtaccttac tcaatcattt agctgcttat ctcgacacta aacagcaacg
    29221 accttacgtt gtccaccgct tggacaaaga aacaagcggt gccgttttat ttgcaaaaaa
    29281 tccatttgtc ttgccgatac ttggaagaat gttggaacaa aaaatgatct atcggagata
    29341 tcaagccatc gtatggggag aaattgccaa agatatgacc atcaccaata aaattggacg
    29401 agatcgccat gatcggagaa aaagggtcgt agatgataaa aatggtcaga gcgccttgac
    29461 acatgtcact gtcaacaaag tactttctga acaaacacaa gtctattgtg ttttggaaac
    29521 cggacgtaca caccagatca gagttcattt agctcataag ggacatccga tcgttggaga
    29581 ccctctctac caaaatagaa cagctgaacg actgatgctc catgcattag aactgcacat
    29641 gatccatcca tttactcaag aaaaaatagt cggtaaagct ttacctggat tatgggaata
    29701 gaaataaaaa agaacagagt cttatcagca gataatgata agactctgtt ctttttgtgt
    29761 aatttgtctg cttacttttg atgttcgtgt tctgctaaca gcaaactacc gataatccca
    29821 ctttcattcg gcagtcccca acgaacgata tattcactta cttgaggtgt ttccatataa
    29881 ccacccatca attcaacaaa ttgctgacgg attttttgaa ggagatgatc ctgattcatt
    29941 accccaccgc ctaaaataat cttttcaggt gctaacgtca acgtatagtt gatcagtgct
    30001 tgagcaatat aataagcctg aatagcccaa acaggatgat cttgcggcag atcttgccct
    30061 ttgatccctg tgcgtgcctc aatagacggt cctgctgcta atccttctaa acaatcttta
    30121 tgataaggac aagtaccttc ataatggtca tctggatggc gtttcaccca aatatgtccc
    30181 atttcaggat gtgcgatccc tgaaaaaatt tcttcattga tcacgacacc tgcaccgatt
    30241 cctgttccca cggtaaggta aatacaacta ttttttcctt gtgctgctcc ttgcttcaat
    30301 tccccatatg cagcagcatt gacatccgtt gtccaagcag caggaatatt gtagcgttcc
    30361 ttgattgtgc caagaaaatc gaattgtgcc cacccttttt tgggagttgc caaaacgtat
    30421 ccgtaattat cttttgtttt gtcgatacca atcgggccga atgagccaat cccgattccc
    30481 ttcaattcat aacgatcaaa aaattcaaat acctgatcca atgtttcttc tggtgttgtg
    30541 gtagggatac ttaatttttc taaaatttct ccaccatctg aaaccgcaca cacaaatttt
    30601 gttccgcctg cttctattcc gccatacatt cgttttcctc ctcgaatccg attgtccgaa
    30661 tctcataagg ttggattatt gggacaaccg cttgcttcgt tggttcttca agcagattca
    30721 atatgacagt ttttccattg tctttagtta tttccaattt ttctaaatga ctagacatat
    30781 tgaaacctct tactactact ttatcactaa acttgctgcg tttcaaagct gtaattgcaa
    30841 acgtttcact ttttatcgtt aaatattgtt gttttgaaat caatgttcct tcatgatgtt
    30901 tgatttgttg agtggaaaaa ggaatctgtg ctgcgtatgc atgcaaataa gtagagaatt
    30961 tttcttctgg tccatgtagt tcaatgctgt aattgaaccg atgctcacct agacactgtg
    31021 cttctggtgt tggaaaatat ccccaatcgc ctagttctcc cacactgcga agtagcgtca
    31081 ctgcgatttg tccgtcttct gttacttcat attcattcaa accaaagttt ccgactgtta
    31141 caccgtactc ttcatcatga atattgacaa atgcttgctg atgttgcgga ttcgtggggt
    31201 tttcccaact tttagacaca tggtttgggc gcttcaccac ttcaaaaata ctatctgctt
    31261 cgtgatgttc cacatgtaac tttgtaggaa acaagacacg taagcgatga tctttcatct
    31321 gattgtcgag agtcgtttca aagtcgattt ttttactatc ttttctgatt gtcatgattg
    31381 tttttatttg aaggattcgt ttttctttgg atcgttctgc ttttcgataa cgaaattcca
    31441 caaccatctg ctgttctttt tctaaacgct cgtctgcaga aacagggatt tccatttcct
    31501 gtagaatact tatttttgct atttccggtg tatctactaa tacttttact tcagagtttt
    31561 ctttgttact ggataggatt ggctgatcac agaaaggttg tttaaagata tactcatttc
    31621 ctatatcgcc gatatcttca aaaatatgca gattattcaa ctttcgtttg tttttgcgat
    31681 cttccattgt aatggtacca tttttttcaa tcgtcaaatg tagagggccg ttttcgatga
    31741 ttcgtccact ctgatgaatc attgtttcct tttcttctgc tttcgcttcc ccttctacaa
    31801 gcgcgaatgt ttcccaagaa aatgctggca tgttttctaa tggtaaagtc actttgatca
    31861 ttcttgccat atatggcaca cggaaacggt ctttcggcaa atcataacca aattggacag
    31921 tttcttcaga taaaatagct ggtatagtgg ccccactttt tgtttctaca tgatacttac
    31981 gtttaggcaa attttctaac tcttgatata gttgttcagg tattccttca gcgaatcttt
    32041 ttctttctaa ttcgatggtt atttctgcct caccagtttt atccattcct gcagtattta
    32101 caatgacaaa tggaaagctg tcttttggga atccggtcgt atcgattgct gcggtcagct
    32161 gttctaagga atcttgtgca agatactttc caacttcatc tgctttttca aaacgaggta
    32221 tcatttcccg atggacagaa tcgactgaac aaccgcaaat actgtcgtga ggatgatttt
    32281 gcattaatgt cttccaagca taatctaatt gatcatgggg atagtttcca ctaacttcat
    32341 aagccatcgt cgcaagtggt tctgtaatat tttctagctg tcgctggacc ttggtattcc
    32401 attgttttaa gtaaacccga gcagatgctg tattggctaa agtataccag ccatccgttt
    32461 cttgacttgt caattctcct tctactgagc ctaagtcttc tggcacatct ttttgaactg
    32521 cttccagata gtcagtaaaa ttagaatgaa taaactcgta atctggaaac agttcatttg
    32581 cgagatggat tgcttttgat atatcttttt gtaccggttg atgatcaaca ccattcatca
    32641 tcaataagtg gttcgtagag gcatattgtt ctgcatccgc taatttttgc ttccaaaagg
    32701 caagagctgc ttctttttct actggaatct cattcccgtt gctataccaa ttggcaaata
    32761 gcagaccaaa gattgctgtt tgatcaggtc ctttccacca catctctgag tattgggaag
    32821 aatagttttc tgcttccaaa acttgattat caaagccaat tggttttaca ccacggccaa
    32881 acgctgctgc tgaaatacca gcttgtttca ttaattgagg cgtctgtccc atatttccaa
    32941 atgtatcggg aaaatagcct aacatgacag gggttcccca tttacggctt tcttccatgc
    33001 caatcagcat atttctcacg ttcgattccg agctgataag aaaatcatct tgcaagatat
    33061 aaaatggtcc aattctcaat tttccttcat tgattgcttg ttggattgcc tgtctttttt
    33121 ctggccttac ttgaagatag tcatctaaaa taatcgtttg cccatccaaa tgaaaactgt
    33181 tgaaggacgg atcaacttca aatagctcta gtagatcgtc tatcaattca atcagtcgca
    33241 tgtgatgctg ttcgtaagcc atgtaccatt cacgatccca gtggctatga gaaatgatat
    33301 atactttctt tttcatttac gatacctcct acttttctac tctaatatca aagtaatcca
    33361 tgactaattc gcaaaacatc atatttgccc aagaaaacca ttctctcgta taattttcag
    33421 gattgttcac atcgaatcct tcgtgcataa ggtgtgttcc tgcgtcagtt gctacaagta
    33481 aattcaatat ccgctctttt tcagatttgt ctgacgttgt cattccttcc atagctaatg
    33541 caatcggcca aacgtatttt tctggcgtat gggaggatcc aattcctttt gcgtactttc
    33601 cttcatagaa atatggattt tctttactca acaatgttct tcttgtttgc agataatctt
    33661 cctcttgttc agaacaatat cccaagtaag gtgcggcgat cagattgggc acattactgt
    33721 catccatgac tgtggcgttt cccattccat ctacttcata tgcataaatc gtttgccctt
    33781 tttgattaac cgttcgtccg tgattctgaa tcccctcttg gatctctttc ttcaatttct
    33841 gtgtttgctg tacaatttct ttatcatcga gtatcgtttg aaaaatttct tccacataac
    33901 ctaacacgac caccgcaaac atattggatg gtaccagata tccataacgg caagtatcat
    33961 cgctaggacg gaaaccagac catgtcattc ctgtaaaagc tgtttttgca cctcgcccgt
    34021 cattgatcag cgtatcttct tttcttgtcg tgtctcgtac aaatgtataa ggagagtgtt
    34081 catgatcttg ctctgttttg aacacgtcca agattttttt gataccttca ataaaagatt
    34141 gattgaattg atcagttttt cctgtatttt tgtaaagaag ataggccaac tgtattgggt
    34201 agcataaaga atcaatttca tattttcttt cccagatcca atcgttcatt tttgtatgat
    34261 ctgtttggtg gccagcaccg ttagcttctt cgttgaaggc attcgcatat ggatcgatat
    34321 tgatataaaa aaattgcttt ttgactaacc cgctgatcat gtcagctaat tcctcatctt
    34381 cttttgcaat cactaaataa ggacgaactt gtgcagtaga gtctcttaac cacatcgctg
    34441 gaatgtctcc cgtcaataaa aaggtggttc cgtcattttg ctttttgact gttgtcaaca
    34501 gagtattggc aaatgctgct gtaaaattct ttgcccagtc cgcatggata tccccacatt
    34561 tttgtgtgat atcttccata aatttctgca ccgattttgg tatatcttta taagccacta
    34621 ataaaacctt ctttctattt gatatatctt taaagattat acttcataca gccaattatg
    34681 tctacgcttt caaaaaatat ctttttattt actttttcat ccattatggt attatttgaa
    34741 tatgatatat cattaagagg tgtttatatg aatcaaccat tgtaccagac aatatttaat
    34801 gatttgcaaa cggctatttt aactggtgaa ttacctgtag acagccaact accaactgaa
    34861 aaagaacttt cacgtaccta taaagtaagc agaatcactt ccaaacgtgc attgactgaa
    34921 ttagagcaat taggacttat ttatcgcgtt cgcgggaaag gcagtttcgt taaatccgct
    34981 tctccggaac agattcttgc ttctcctaca aaaacacgac ggattctgtt tcttcttcca
    35041 tatgtcgctg atttgtctgt tggtgatttt agcaagggat tgaaccctgt gatgcaagat
    35101 aaacagattg atgtcatgat gacgacgctt gactttctac aaaataaaac agcaaaagag
    35161 ctaatcaatg aatttgacgg attgatttat tatgctcttg atacagaaca acatcttgac
    35221 ttactttttg agctttcatt gaaagaattc cctgtcgtca tattagataa aaaaatctat
    35281 gagttacctt ttcctaccgt tctttccgat aatttccaag gcggtgcgct tgctgcctct
    35341 tatttgatcg aacagggaca ttcaaaaatc gcttatttgt ttggtacgca gactcatcca
    35401 caatccgttc gacagcgtta ccttggttac cttgaagcat tggacaaagc tggtttgact
    35461 ttccacactt cactggatga caaaagcgcg acattagatg aattgctttc ttatatcact
    35521 aagtatgaag taactgcttt tgtttgtgaa aacgatgttg tcgctatcca gaccatgaat
    35581 ctcttgagac agcatggtta tcatatccct gaagattttt caattatcgg atttgatgat
    35641 atccaagcag cagctcttgt tgacccgcca ctgacaacca tcgcacaaga ttttgaacag
    35701 ttaggtaagc ttgcgggaga tgctttggtc acttggttag agacaaaaga tcgaccatcc
    35761 gatagtaaat ttccagtttc attcatcaaa agacaatcaa caaaggagaa ataaaatgaa
    35821 tatcggtcaa atagatacta gacatgcaac agcgaatcag ccaaatttct ctaatgggaa
    35881 ctgccagcct tatactggcg ttccttttgg gatgaattat tttgcaccgc aaactacgga
    35941 tcaaaacggc agctggtggt ttcatcctga tgaccgcgtt tttcaaggct accgtcttac
    36001 ccatcaacca agtccttgga tgggtgattt cagtcatatg ctgtttacgc cgatcaatgg
    36061 aaaactacag gagaacaccc ttttccatgc tcaaagttct taccgcccag aagaaagtat
    36121 attttgcccg actcatctgt cgatctgtca attacgttat ggcatctgtt ctacattgat
    36181 acctagcatg tatggcggaa tcttatctat tgattacagc aaaaatgaca gcggtttact
    36241 tttatccttt ccgggacgcc atcagctctt tgttatagat ccttacacag tggaaggaaa
    36301 aatcacacat tttgctggtt cagaagatcc tgatttttct ttttatttta tattgagatt
    36361 tgaatatcct ttaacaattg atgagttagc tgtttccaac aaaaatagcg actcgatccc
    36421 gttgtatttt gaacagacaa aaaaacaaac tatccgattt gggacatctt ttatcagctc
    36481 ggaacaagca gcttataacc tggaactaga acaaaaccaa agcaaagaga cttaccttgc
    36541 tcacagtcaa gaacaatggc agtcctattt tgatcgtata cagctcacac accatgatca
    36601 acaacaattg cggacgtttt atcataattt atatcgggtg tttttatttc cacaaacatt
    36661 ttatgaaatt gatcctagtg ggcaaaaaat ccactatgat acgactagtc gatctataaa
    36721 acctggtgtt ctctatacaa ataatgggtt ttgggatacg tataaaacgg tttatcctct
    36781 ttattctttg attgctgtag aaaaatacga agaaatgctg gaaggttttt tgaacagcta
    36841 tagagaaagt ggctatttgc caaaatggct gtctcctgat gaacgtggac ttatgcctgg
    36901 gacattgatc gatgcggtca ttgcagatgc tgctgtaaaa gatatccgac gagacttgat
    36961 gcctgaattt ctaacggcaa tggaaaaagg tgctacggtt caaagcaaac atccaaatta
    37021 tggacgccaa ggaacagaag attacttgac atatggctat gttcctaatc actaccacga
    37081 atcagtcaat cacacattag actatgctta tagcgatttt tgtattagcc aagttgccaa
    37141 ggtactagaa gaagaggaaa tgacgaagaa atatcagcag caatcattga actatcaaaa
    37201 tatctttgac tatgagtcag gatttatgcg ttcaaaagat aaagatggca agttcagaga
    37261 accattttca gctaatcgtt ggggacaaga ttatgcagaa ggaagtgctt ggcaaagcag
    37321 ctttgctgtt tatcacgatt tttcaggact gatcggaaaa tatggtggca gtgagagata
    37381 taaagaaaaa ctcattgagc tgtgcaatca gcccccaacg tttgacccag gaggatatgg
    37441 ttttgagatc cacgaaatga gtgagatggc cgcgatagaa tttggacaat tagctatttc
    37501 aaaccagcca agttttcact atccttactt gttcagttat ataggacaac ctgaaatggc
    37561 acagccactg cttaaacagt tgatgactca gaccttcgac gattctccaa tgggttaccc
    37621 tggagatgag gacaatggaa gtatggccgc ttggtatatc ttcaatagtc tgggatttta
    37681 tcctgtcacg cctggaaccg gtgaatatgt tatcggtatg ccattaattg acaaggctgt
    37741 tttgcttttg tcaaatggaa aggaattgac gattgaagca acgcctaatc ggccgcagca
    37801 acaatttatc cataaaatca aacggatgga caaagaatat acgaaattat attttaccca
    37861 tcaagatttg ctgaaaggcg gcacgatctc ttaccaatta gggatcatcc ctcaccctaa
    37921 gaaatatcaa gcaaaagatt tgcctttttc cttatcaaac tataattaac aagaggttgt
    37981 gaaaaagctg tcctgcatca agtaataaga acagccaagc aaaaatagat cctcatattt
    38041 ttcgtttggt tggtcttatt accgcagatg caagcttttg aacaccgttt attgagaggt
    38101 tgtgaaatca gcgttctgac ctgagtatta gagagacaaa tggcaaaata gttcctcata
    38161 ttttgccatt ttttgagcta ataccgaggg tcagctgatt gaacaccgtt tagtggagag
    38221 gtcgtgaaaa agctgtttag tttcgagtat taagaaaaat tttgtgaaaa tggctttcca
    38281 catttttgca taaatttggc ttaataccga gaaactggct tttgaactcc gtttatcgag
    38341 aggtcatgaa aaagctgcct tgcctcaagt aataagaaca gcccaaaatt agatttctag
    38401 acaatttttt ataagttatt tcattacatt ggctttttct tttatgattc tccactgttt
    38461 tctcggtact ctttagggga tctgccgacg attttcttga ataatttaga gaaatacgtg
    38521 gtgttgttgt agccaatctc acttgctatc tcattgatgt tttgattact gtgcaaaagc
    38581 agttgtttcg ctttttccat acgtaaatgg ttcaaataag ctgaaaaact ttttttcgtt
    38641 tcttttttga acagctgtcc tagatacatg acatttaaat gcaggttttc tgacacttct
    38701 ttgagtgtaa gtggttcttg ataatgctgg tgcaggatta cgattacttt ttccacattg
    38761 tcactgtact ttttctgtct ctgatgcttt ttgatgaatg acagaagtag ctgctgaagt
    38821 tcttgcaccg tttttgcctg atggattttt tcgattcctt gtaaatattc ctcatcttcc
    38881 agatcaatca attcccgttg aatctccata aacagtaaaa aactggaata tcgtatatct
    38941 tccggcatca tggctgctaa ctgaaactgt tcaaaaaaat cagtgatcat tttttgagcg
    39001 acttctaatt gtttattttg cagcacgcgt ctgaaacttg aaaaatcgat ggtttgatct
    39061 tttgtttctg cttgtactgc gtaaaaaaca tgctcgtctt tatctccata aaattgatgc
    39121 aactgtagat tgtctttcgc ttgttggaat gtttcaggga ttttgtccaa agaatctgtc
    39181 tcttccccga tactgatcag ccattcctga tcaggaaagt tttcttgaac aaaacaacag
    39241 aagccatcaa cttcatctgc ctccaacaac aacgtgatca atacacggtc tccgtaattt
    39301 cgttgatagt aaaagatttg ttgccgttct tgcaaccatc tattgataag agtatcatga
    39361 gttcttgcca gttgaatcaa tactatccta tgctgcttct cacttatttg tcctattatt
    39421 tcttcttcac ttatttcatc caattcttca ttcaaccatt gactgaataa cagttcttga
    39481 tagatttctt gttgattttt ttgttcattt tgctgatcta accgtgtttt gattttcttg
    39541 aggctttcaa ccaattcgaa cttattcacg ggtttcatca aataattcac ggcgcctaat
    39601 tgcatacctc ctttcaaata atcgaattct tggtatcctg ataaaatcat aaattcaaaa
    39661 ttatgacgtt ctttttgagc cgcttcaata aattccagtc catttatttc tggcattgtc
    39721 acatccgtta ctactaaatc aatctgtcgc tgttcaagta cagctagtcc ttctttggca
    39781 ttctctgctg ttgctactat ttgaaagcct aaactttgcc aatcaattat cttttttaac
    39841 ccattcaaaa tcatgtattc atcatctacc aataacactt gatacacgct catccctcct
    39901 tgaatgtaat agaaacggtc agtccacctg tctcattatt ctcaagcgac atataataac
    39961 tgctgccgaa gctggcacgt aaacgttcat tcacgttttg cagaccgata gaaccgtgaa
    40021 gttccacttt aggatgagac agtttttctt caatctgttt tagccgtttt tcagtcatcc
    40081 cttttccatt atctttgatg atgatcctta cccgtttgcc ttcctgcaag actttgacac
    40141 ttagcgcatt atcgaatcga gtaaaatcaa taccgtgttt aaaataattt tcaactaatg
    40201 gctggatcac aaatttaggt acttcgatct tttccaaatc aggatcgatc ataaaatgat
    40261 aggctactcg attaggataa cgcatttgat acaaatagac atatttttcg caaaagtcca
    40321 gttcttcttt taaagtgatc gttttctctt gattcgtatt gtttcgaagc aaagcggaaa
    40381 acgcatacac aacatctgct aattcttcac ttccttcgct gattgcatac attcggatat
    40441 attctaatgt attatataaa aagtgtggat tgatctgtgc ttgaagcgcc cgcatatgtg
    40501 cgtcttgctg tttgatttct aatttgtaga tgtcttctac aaactgattg atgctatcca
    40561 acatttcgtt gataccgatt gaaagttcct tcagttcata ttccgtgttt gttatgtcga
    40621 tccgcgtctc taaattccct gctgctgttt gatccatggc ctgcatgacg atagatactt
    40681 gctgtgaata tcgtttgaat gttttataaa gaaagataag cagtatattg tctaaaataa
    40741 tgataccaat gatcagcggg gcaagatttt ttcccgtttc gatttttaca ctttttttat
    40801 ctagtacagc taatatacga taatcatcag acagttcttg gtgttgaaca aaattgtttt
    40861 tttccagatc gtcaatcggc aattgtgaat ttttcttcag actctctgtg atgatcattt
    40921 cttgtctttc aaacgtttct ttagctattt tattatcatg gaatgtgtat agtcgatttg
    40981 tcgttccaga aatcatgtat aaagagagtc cattgaatga cgttagattt tctaaaatca
    41041 tatctagttc tttcttttga aaaccaataa aaatacttcc tgactcagct cctccttgtg
    41101 tgataggcaa cttgatataa aaggcgtcta gtaattttgg tgtccccttt tttatttccc
    41161 cacctttgtt ttctctagaa gattcataat actccggtag ttgattcaac acgatatata
    41221 tcgcacttag ttcttcataa tttgcgtaga gatctttgat ttgatttggg aaagaaacta
    41281 attcatcggt actgttttgc tgttcgtata catacatcag atactgatct atgggttttg
    41341 ttaagtaggt cgtcacattt tcgatctttt cggaattccc tacaagctgg tttttgatga
    41401 tcctactcaa ggttgtttta tcattcagca ttctgcttgt gctttgaacg gcatcacttg
    41461 tcatgttttc tgcctgtttt tgattttgtt cccaagtatg gatactaaaa ataacggtag
    41521 cagtcatcgt tacaccgatc atgataaccg cgtatttccg caacagacga tttagtaagg
    41581 ttccttttga aaatagaatc cgaaacttat tcataacttt ccaactgttt atctaattct
    41641 tcacgccaat gtgttgtcat cgtaccattc caaaccgtca aaagaccaat gacaccaaac
    41701 aggatcaatc ctttgaattg ccaagtcacg cctaaaatta taccgctacc aataattatt
    41761 tttagaaaag acgaaaagct ggaaaaaacc gaaataaaac ttagctttag cagattagga
    41821 acagagatcg tatattcact atctaaaatc atactgtatt gataagtagc aaataggcag
    41881 agcaaggcaa aaagtaaaat aaaatcaata gctaaaaaaa tgagcccttt gatttgaaca
    41941 gataaatata aattgtaact caataaagca gtaccagata aaaataaact aaatagcaga
    42001 ttgcctctta aaaaatttct tttatatata ttccagcctc tttttattgt gatttcttta
    42061 tactcgaatc cgtacaaata aaatagttca ttgactgttt tccatgccgc acctaatcca
    42121 aagatgatcc cgccggcaaa tgtatatatc cagaaataca aggttagttt tataatgacc
    42181 cagatccgga taaataactc ttccaaaacc ttccctaaca tatcttctcc tccaaacatt
    42241 aagtttatac tttctcatta tacaaaaaaa ggttgggcaa tcagcacctt gacctgatta
    42301 ttagagaaac aaacggaaaa atagttcctc atattttgtc gttttgtgag cgaataccga
    42361 gggttagctg attgaacacc gtttaccaag agtttgtgaa atagccgctt agtttcgagt
    42421 agtaagaaaa attttgtgaa tatggctttc cacattttta catatattta gcttaatacc
    42481 gagaaactgg cttttgaaca ccgtttattc ggaacaagag gttgtgacac gtcttttgtc
    42541 acaacccctt tgtctttatt cattattatt cttgtcggaa ctcatcatat tgtttttgca
    42601 tttctttcaa taccttatca tagccagcct tgtccaaagc gtctttcaat ttcggtaatg
    42661 tttcatctgg atcaacagtc cctgtattca atccatctaa atattgattc atgacattag
    42721 aaatattact taattctgtt ttgacactgt cagtgttgaa gctgaatcct aaaatcggtg
    42781 aagtttctgc atcagcaatc gattgatcac gtttggcaat catgtcatcc gtaatagact
    42841 cttgtgtata tagaatcttg ttgtttcctg tattccaagc agacatatgt gtatttggct
    42901 gataaccatc tagcaatttg attttatcat ctgaaccatc gactttttcc catgcttctc
    42961 cctcgatccc ccaaacaagc ccattcaata attctggatc actgttaagc agccctagaa
    43021 gttctactga tttttcttta ttttttgaag tatttgatac gacaaaattt gccatttgtg
    43081 cttgagctgt agattttagt ggaacagtaa ttgcttttga gacaagttct tgttttgctg
    43141 catttgtcaa aattgtatcg ccataatcat acggtccttg tgtttctcca cgcattagcc
    43201 aagtatttcc ttctagtgga taatccttat tgctcgttgc agcgtcactt ggaatgagtc
    43261 cttgtttgta ccagctatgc attgtacgta acagtccgat atacgtttca ttatcgtatt
    43321 ggttgatgat tttcgaatca tctccatcca gatcaatagc aaatggtaac gtattcccta
    43381 aaggataatc aaagttccct tggactttga agccttgtcc aatggcaaaa gctgccgtgt
    43441 ttggttcttt ttcatggaat tccttcaaga ctttttctgc atcagcgtaa gattggattc
    43501 catcaattga aagatcgtat ttatctagta aagctttgtt aaaagtaagt acttgttgag
    43561 caaagacatt tccattgact gggaaagcat ataattttcc attgaccaga tttcctttaa
    43621 tatatgcttc atctaaatca ttataagctt tttcagcgta cttaggcgct aattcagtca
    43681 aatcagcaaa ggctcctttt tgggcattgg aaacatagtt gttcgcaaag gcgatatcat
    43741 aattttcccc ggaagaaatg atgacgttca ttttcttttc gtaatcgccc cagccaatgt
    43801 aattgatatt gacttttaca ccgatctctt tttcgattcg tttgtttgct acttccatca
    43861 attgatcgaa attatctggt ttatctccga tttgatacat ctgcaaagtg tccgaattgc
    43921 tagcttctgc ctggtcttta gaaccagaac ctccgcatgc tgctaatgtt cccaacaaca
    43981 acgctgaaac acctgtaaag gctagtttct tccataactt catttttctt cctcctaatt
    44041 ttatgattta tatttttgtt tctttattct tttactccgc cgattgttag acctttgaca
    44101 aaatgcttct ggaaaaatgg atagctgatc gcaataggca acgtcgagat gactacgatc
    44161 gccattctgg cagattctcc tggaactgag gctaatccgc ctgtcaattg gcttcctgct
    44221 cctgaatttt gtgtcagata ttgaatatta ctttggattt tcatcaaaag atactgtagt
    44281 ggaactagct tatcatcttg gatgtatagt aaagcattga accagtcatt ccagtaaccc
    44341 aatgcggcaa ataaactgat tgtcgcaata cctggaacag ctaacggtaa aacgatctga
    44401 acaaatattc gcagttcgct agctccatcg atccgtgccg attcaataat cgaatcagag
    44461 accgaacgtt taaagaatgt gcgcatcaca ataatattga agggactgac agccattggc
    44521 aagatcaatg cccaaatcgt atcttttaac tgaagaagat ttgtcataac aagataattg
    44581 gccaccatcc ctggtgagaa caacatcgta atcaaacaaa aaacggtaaa aaaacgacgg
    44641 aatggaaagt ttgaccgtga aatcgcataa gcatataaag aagtagccgt gctattgatc
    44701 agtgtaccta aaacagtaac aagtacagtg acgaaaagcg cttggaaaat tttatcctgc
    44761 atctggtcga acaagtacgt ataaccaaat atactgaact tttctggcca aaaactgtac
    44821 ccgtgagtcg acaatatgga ctcatcggta aaagagatgg cgataacaaa tataaatggc
    44881 aaaatacagg aaatagcaaa gagtgcaatc attatattag agaaaaaatt agccgtctta
    44941 ttgaaagagc ggacttggac tctctccact ttcttttttt tcattttttt cctccttaga
    45001 ataatgcaga atcgctatca aaacgtcggg cgatcgtatt tgtgatcatc aacaagacaa
    45061 agccgacaac tgattggtaa agtcctgcag cagccgtcat accaaaatct cctgtggatg
    45121 tcaggccatt gtagatatac gtatccaaaa cttgagtcac atcatataat gcaccggaat
    45181 tcctaggaat gttataaaac aggccaaaat cagctcggaa aatgtttcct acggctaaaa
    45241 tcgttaaaat cgtcattagt ggaataagtt gtggtatcgt cacatgtttg atctgctgcc
    45301 atttgctagc tccatcaacc atagcagctt cataataagt cggatcgatt cccataacag
    45361 atgcaaagta aataatgctg ttataaccaa tccctttcca tgtaccaatg aacaccaaga
    45421 taaacggcca atatttcggt tcattatacc aattgacagc ttctcctcca tgtccagtga
    45481 tccactgatt gaatattcct ttgtcaggac ttaaaaaagc gtaaacaaag taactgataa
    45541 tgacccaaga tagaaaataa ggtagtaagg acatcgtttg ataaacttta acgagtcgtt
    45601 tatttctcaa ctcgctcata atgattgcaa atacgactga gatcagcaga ttcaaaagga
    45661 taaatgctac attatataaa attgtatttt tagtgatgag aaatgcttga tccgaagaaa
    45721 ataaaaattt gaaattttcc caacccaccc aaggactttc cctcaagcta gccaagaacc
    45781 catctgctga aatatgaaaa tttttgaatg ctacgacatt cgccattaca ggtatgtaaa
    45841 agaaaaaaat catccaaagc atacccggta cagccatcag gacaagtgct ttataacgcc
    45901 atagattcat aaaaaaatga ttgaacccct ttcgtttttt catctaaaaa cccctttcga
    45961 ttcattcgca acgtaattat agcgctttca ataatgagaa taaataaaca aagtataggg
    46021 aaaatgaaac aaagtatagg agaggttgtg aaatcagcgt tttggcctga gtattagaga
    46081 aaaaaacggg aaaatagttc ctcatatttt gccgtttttt gagctaatac cgagggtcag
    46141 ctgattgaac accgtttatc agaggttgtg aattcagcgt tttggcctga gtattaggga
    46201 gatcgtgtcc ttactatcta actctgagtc acaaggaaca atggggactg taacaaaagt
    46261 cttgtcacag tcccttattc cgaacaaacg gtgttccaga agtagcttct acggaaataa
    46321 gccgaaattt cacaaaaaag ccatccgaaa gtcagacgga caagtctgct tttcagatgg
    46381 cgctacaact actctgctta ttcttttagc tatccattca attatttttc tctcacttgt
    46441 tctagctctt ctttggtcat gggtcgatat tgccctttat tcaaatcttt aggcagccaa
    46501 aattttccca tacggattct ttttaaatat aagacttctt ttcctactgc tttgaccatt
    46561 cgcttaactt ggtgaaactt tccttcatgc aaaatcagac ggataattga agtttctgtc
    46621 tcttgatcta cttgatcaat ttggagtttt gccggcaatg tctgctcatc gccatcaata
    46681 atcagtcctt ctgcaaataa acggatatct tcttctgtca ttataccgct aacctcagct
    46741 agatattctt tctccacatg ttttttaggt gatagtaact gatgggcaaa caccccgtca
    46801 ttcgttaaaa tcaagaggcc ctctgtgtct ttatctaatc ggccgactgg aaaaagatct
    46861 tcccgataat cctggtcttc caataaatcg attacggttt gatcatgatt atcctgagta
    46921 gcagaaatga ctcctgcagg tttatgcaaa atgtagtaaa aatctttttg atacgtaatg
    46981 atttctccta aataagtaac cgcatcttcg aattcattta cttgcgcttt atcgcttttc
    47041 accacttgtc cattgacttc gatcgttcct tttttgatta atcctttgac ttctttgcgg
    47101 ctgccaagtc ccatatctgc taaaaactta tctaaacgca tgcgtttccc ccatttcaaa
    47161 agagcatcaa tctagctctt ctgattcgct tattttattt ttaatttatg tcgtaaacgc
    47221 gctattcctg gtcctagtag tctttctgcc aagcggattt tcaaagctaa ataaccatac
    47281 actaaagccc ctatacctgc tactacaaga atcaacaata gagactggaa tttgctatct
    47341 tcgtttaaaa aagcaccaaa aatcatcttt gtgatccacg aagccaaaag cataatcaag
    47401 gtaagaataa agatcagcaa tgtcctgcgc cagacaaaac ttggtttaaa gcgggctact
    47461 tcatggattc tctttaagat caaggtacaa gacacagcaa atccgatcat tgtcgccaat
    47521 aaagggccgt atacttcaaa taaacgaatg gctgggtatt gaagaaggag tttcactaga
    47581 aacccaataa ccaaataatt aattgccgcc ttgttttcaa acattccttg cagcatgttg
    47641 gaaactagca tatatagtcc taggaagagt cctacaaaac ttgcttggat cagtacatta
    47701 cttcctagag aatccggtgt ataaaataat gtatttagcg gataggcaag taacataaca
    47761 ccaaatgtcg ccggaaacat aaaaaatgaa aacaactgta aatttccgct tgtcaatttt
    47821 gccagaccta ctcgatcttt caacgtaaca gcttctgtaa tcaacggtaa cccagtagac
    47881 gcgatcgatg ttgccaatgc aataactacc atcgtcaact tatctggatt ggcactaaaa
    47941 atcgaaaaaa gatccagtaa ctgtgcattg gaatactcgg tggatgcaga catgatcttc
    48001 ataaaagtga actgatcgac taatttgaag atcgttaccc ctgaacccac aataataaac
    48061 ggaatcgctt ctttaacggt atctaacaac aattccttcg ttgcaatatt tattttgttt
    48121 tcgctgtagt gaataaacgc cgatgtatac gcttgatgtt tcttcaaaaa atacaaaagg
    48181 acaagaatac ttgctagcat accgataaat gccgcgaaag ttgattgcgt gaccgccgtc
    48241 acatagtcac catccatgac tttcatgatg atgaatgtag ccaatagcat ataaaaaata
    48301 cgtgccacct gttctacaat ctgcgacagc gcataaggca tcatttcttg attgccttgc
    48361 aaataccctc gtatcacact catacaagga aaaaccaata cagcgatact caaagaacgc
    48421 atcgttggaa tcagttcttc tccgccacca gaagcatgag ctagccaagg agaggcgatg
    48481 tacatgaaca aagcaaacaa caggcctaac ccgcccatca tctgtaatgc acggataaat
    48541 aatcgtcgtg aagtgccata ttcattcaat gaattatagc gtgcagtttg ttttgcaatc
    48601 gcagcaggta ttcctgctgt agagatcagt agaaacaatg catatatatt gtatcccata
    48661 ttgaataatc cattggcagc cttagcattt tcgcccatcc aagcatacca gggaatgata
    48721 tagacagcgc ctagcaatct tgaaatgata ttactggcgg tcatccaagc agagcctcgt
    48781 gccatttttt cttgcggtgt taattgatct aatgctggtt ttctttgatt atccatcggt
    48841 cagattccaa ctttctacaa aacattacta tcattttaat tggtaaaaca gaaactggca
    48901 atcgatttta tataactttt ataagaacaa ttgttttttt gaattcggtt tcgttttttt
    48961 ggtaaagtaa agaaatatac agccttttat ggctaacatt tataggagat atcaatgaca
    49021 caagaaataa aaaaactacc cgttatattt ttaggtcttt tctttatcac agttggattg
    49081 aattggttct tgctccctca cgatatagct tccgccggag tgggcgccat cggacattta
    49141 gttgaaacgg ccttttctat ccaacgctca gttactgttt ggagcatcaa tcttatgatg
    49201 ctgcttctag ccgctgtcct attaggaaaa tcagtatttt ttaagactgt agcgggaagt
    49261 ctgctttttc ctgttgtttt agagattgtt ccaaaagtgg aaatgatctc ctcacatttc
    49321 ttttcactga tttcaggaag tcttttgttc agtcttgggg tttacacatt gtacacagtc
    49381 ggggcctcaa atggcggtat aacgataccg ccgattattt tccacaaatt ttttcggctt
    49441 ccaatgccgc aaggactttt acttaccaat agtcttatcg tctttttgaa ttattatgtt
    49501 tttggattgt tggagacact attcgttctc ctatctatta cagtcagttc attgtttatg
    49561 aaaatactca tccgcttgtc ccctgtgtca agaaaatttg aataaagctg gattttgtca
    49621 tagcggaaac aaacgatata ttaaaaagag tgtgctataa taaaccaaga aattgaataa
    49681 agcgagggat caatcaatga gcttctcttt agaagacatc cgtcatttac tattaaaaga
    49741 aaatctatta aaagaatttg tttcaacaag aggctggcat cttgatgtac cagaaaaagc
    49801 tgaatttctt cagttaagtt atgattcaag aaaagctgat gcttctactt tgtttttctg
    49861 taaaggaatg aatttcaaag aagaatattt ggattcagca atcgaacagg gtattcaata
    49921 ttatgtatca gaacaaccat acgaaaattc agcagtagga atcatcgtaa cggatatccg
    49981 taaagcaatg gcgcttcttg ctatggcttt ttacgactat ccgcaaaata aattgaaagt
    50041 gatcggattc actggaacaa aaggtaaaac aactgctgcg tactttacaa aagcaatctt
    50101 ggatcacaca acaaataaaa aaacagcatt gctttctact atgaatacaa cgttagatgg
    50161 caaaacatat ttcaaatccc atttgacaac gcctgagtca ttagatttat atcgtatgat
    50221 gtcagaagca gttgaaaatg gtatgactca tctagtgatg gaagtttctt cacaagctta
    50281 caaaacacaa cgcgtatacg gcttgacttt agatgtcgga atcttcttga atatctcgcc
    50341 tgaccatatc agtccgatcg aacatccgac atttgatgac tatttctatt gcaagcgaca
    50401 attgatcttg aattcaaaaa cagttgtttt aaatcatgaa agtgactatt ttgatttact
    50461 tagggaaaca gccgatctat tccagattcc aacgatcact tatggtcgtt cggaagaaag
    50521 caattatcaa gtgatccgtt cagataaagg tactcacggt tttactttat cttctcatga
    50581 agatcgctta gctatctgcg atactgctta tgatattttg ctggctggag gattcaacca
    50641 agaaaatgca gccagtgcga tcattgccgc agcactaaca ggtgcctcca gtcaagatgc
    50701 acaagacgga ttaaaagaag ctcgtgtacc aggccgaatg gatcaattga tccaatccaa
    50761 tggagcgcat gtctatgtgg actatgcaca taattatcta agcttgaaaa cattgctgaa
    50821 atttgccaaa aatgaacatc cagatggccg tgtaattgtt gtattaggca gcccaggaaa
    50881 caaagcaatc tcccgtcgac atgattttgg aaaagtgcta tcagaaacag ctgacatcgc
    50941 ctttttgacg gcagatgacc cagcatttga agatccgaaa aaaatcgcgg aagaaatcaa
    51001 tgaagcaatt actaatccag atttgatcgt tcattatgaa atggatcgtc ctgaagcgat
    51061 tcgacgcgct ttagcagaaa gcgctccgca agattcagtc gttattgcgg gaaaaggcgt
    51121 ggatccctat caaaaaatca atggtgtaga tgaaccttat gaaggggatt atgcaatcgt
    51181 gaagcggttg attgaagaat aatggtattt atatactagt ggtttcccat ttctaagaaa
    51241 aaaagggact gtgatttttt gtcacagttc cttttttctt agaaatcatt ttaacttttc
    51301 actagctaat tctgctgcct tttctctttc ttcatttaat aatttattat catagaattt
    51361 gataaatggg aaccagataa tgaatgcgat gattgcacaa atgactgcta acacagcagc
    51421 tttccaatca ccaccactac caacaaatgc tccgatcccg actggtgaag gccacggcat
    51481 ctgtgcaagc atcggtttga cgatttccag ccgaatggca aaataagcga gtgtagcaga
    51541 agccatcgga gctaagaaaa acggaaccgc taaataggga ttgtagacga ttggcatccc
    51601 gaaaatgatt ggttcattga tattgaataa tcctggtaca agtgaggctt ttcctaacac
    51661 tttcagctga tcagatttcg cgaggaatgc aataaagatg atcaatccta aagtcgcacc
    51721 tgatccacca acggttacat aagaattgtt gaattctcct gccaacggat aatttgcgcc
    51781 ttgtgcgtta gctgccatat tagctaatac gatcggggtc aagaaagaag tgatgatatt
    51841 cgctccatgg atccctacga tccacaatgc atggatcaag aaataaatca ccatgacacc
    51901 aagccatgta ttcgtcaact gcgtaacaaa cccaaatggg atagcaatca ttttgaagat
    51961 atccgttcct agtgcaacta ataatccatt gatgatcatc accacaaacg caatgacaaa
    52021 tgttgggatc agagcagtga aagaacgaga aactcctggc ggaacaacat ctggcatttt
    52081 tatgacccaa tttctcttga cacacataca atacaattcg accgcaataa tcgacataat
    52141 aatagctgta aagattcctg aagttcctaa acgttcgaca aaagagccca tcctaatacc
    52201 atttactaca gtttctgtct cattgataga agcaatcaag ctgattttac catcagtcat
    52261 gatcaattca gggatacaca taaagaatgc aaaaacagat agtaatgccc ccgtgagcgg
    52321 attaacattt aatttttgtt catctcgttc aattgacgtt aattcataag caaacacaat
    52381 ggcaaagtaa atagataaaa tccccatcgt cattgtattg acgatcatgt acaagtcact
    52441 aactttgaaa aaagttgcat taaaaaatcc ttctaaaaat gggaacggta atggcaaaat
    52501 atttaaaacc aaaaacatcg agcctacaat cgtgaaagga atagaagcca tccccgctgc
    52561 catgacagcc cgaacgattt taagttgcgc aattttcccc ataggtccca tcaggtattt
    52621 ctgcaaaaaa tcaaacatta gttattctcc tttcctaaat ataacgcttt ttcatattgt
    52681 ttaatacgga tatactgttg gtcttggttg agtgaaattt tctttaaacg atactgtata
    52741 aatgcactgg caaaaatacc gcttacaaca agaatgaaaa caaataactg tcctccattg
    52801 ttatttgcca taaaaggata caagaatgta aatcctgagg taaacaacac tggaatcact
    52861 aataaattaa agacactctg gaacaaaaaa taccactttg tccatggaac gttcgtttgg
    52921 tgttttcgat aaagctttac ttgttctcct actgcaggca aaatagctaa aatcagaatc
    52981 atcggtaaac tacttcctaa acttttagct gatagaagca gaatcatcca ttgcatgttc
    53041 atgaaaaaga aaaaggcagt caggtaacgt acgattagat aacgattgaa atacatgttt
    53101 ttcaaactaa gttgcttttt ttctaattcg attttccttt ttactgtatc ctgcacaatg
    53161 atccttcctt tctatttatt tttttgatac aaataattca tttcctgtgc aacttccaat
    53221 aatgtcatcg tcgtcatcag atgatcttgt gcatggacca tgataatttc catttctatt
    53281 tttgtcccgc tggcatattc tttcagaagt tgagtttgtg actgatgcgc atgaagaatc
    53341 tcttcatttg cttgtcgcaa cttctcttct acttcttcaa acatctcttt acgcatcgag
    53401 cgaaatgctt catgtatcag tgtccgtgca tttccgctat ggaaaatgat atcaaaagaa
    53461 gtcacttgta gtgtttcact gtttattttt tcagccatct gttttcctcc ttattcgatc
    53521 agtttgataa atttactgct gaaattctca aaaataggtt cagctaagat ttcctgctgt
    53581 acttttagct ggtcaactaa cctaacgatt gctttagtca cgaccgtaat tccttcattt
    53641 tcgacataag aaggcgaaac taaaaaaaca aaatgaattt cttgatattg atcccactgc
    53701 atcccagaag gaatcaatgc aacggctacc tttgtttcga ttccaactgg aacagcggga
    53761 tgtgggactg ccactgtatc actgaatatg atttgtccca tattttcacg ttgctggatc
    53821 tgctttcgca tttctcttat atatccttct tcttcattta tgctcagctt ttgcagcaaa
    53881 tcatgaagaa tttcttcctt tttcggttcg tacgtataag tcttgaaaaa tttttcagac
    53941 aactgttcgg tgtaaatctg ctttttttgc tgtagagaaa atgctggtat cggtctatct
    54001 gaggaatgat agggtataga ttctccaact acctttcgta ttttacgaac atcatcttca
    54061 ttcaaaaaaa tactgacatg taaaactgga attttgaaaa acattgtcga aagatcaata
    54121 gaagaaataa tcaggtcgat tccttttaaa gttgtttcat tgatctcata atagcctttt
    54181 acacttacca ctgtaatatt tttaccgaat tcactgacta cgcgattctt taatagttgg
    54241 gcactgccgt aacccgttgc acagataatc aatgcatgta gtttatgcgc agctctttct
    54301 ttttccaatg ccgccatgag atgcaaagcc aaataggccc attcatcttc attgatggta
    54361 tagtttttca aagccggcat gttgctgaaa tgccttttta ctgcgtcaaa gactttggga
    54421 ttttctttct ttatctcctc agtcaatggg ttctcaatgc gaatacctcg gctcaatcga
    54481 atcatcatcg gtgacaaatg atcgagtagt ccattgaaaa gctggagatc ctctactaat
    54541 gaaaaatgaa attcctgaga caatcttttc agaactttcg ctaattcctt tgatagttcc
    54601 tgatcatttt gattttcttt gtgattcgac ttagccatga gatgtaatgt caaataagca
    54661 atttcttctt cgggaaacga tacacctgcg attacctcaa tacgttgaac gatccgctgc
    54721 gccacttgat actctactcg cttagaaact tcttgttcga tgcctaattc cttgatttca
    54781 aacccttctc ggagccgttt gatccccaaa gccaagtgta aagtcagatt ctgtatgata
    54841 aaatccgaaa cttttaactt agcttctctt gtttcatcga gaatgataat gactagctct
    54901 tcaaaactga tgtctgggaa aaaacggctt tgccctaaat attctttcaa tgaatttcca
    54961 taatggtttc caaaaaaagt atccatgata aaatgccgtc gatttctttc ttccccttcg
    55021 atccatgtcc cttgaccatg cttagaaacg atttttagtg aatatggaat caatcgctcc
    55081 ttgatttctt gcatgtcctt tgtcaaactg gaacgactga tataaagctc ttctgccaaa
    55141 tcatccataa aaagaatggc atcttccaaa agcaattggt tcaatatata cttttgtcga
    55201 tcttcggact cataaaatcc ttgcggttct ctagcatatc caggtactat ttcttgccga
    55261 ctcaaaaaca agtcaaatgt cagtctttgt aaaatatgca gttggtagcc ataacctggt
    55321 ttagcaatga tcttacctcc gtttttttct actaactctt tgatccgctg caagtaatta
    55381 cggattgtcc gatcagataa taacaattca gaagctaaac gttgactggt gacatactcc
    55441 ttcttgtgat caatcaaata tctcaacagc aatttttcct tctgtttcat tatcatccac
    55501 tcctattcca tcatatgttc tcttcgatca cctctgctaa cttctggatg cctgtaggga
    55561 ttgggacata cgcttggaaa gggatactaa caacaggctt tccaacagat tctcctaatt
    55621 ttttgaattt atccaaaaac atcgtcgttt gcgggctgat cagaaataaa tcaaaatcac
    55681 tattcttaat catattatcg ccttctgtag ctgaaactgc atctacgagt acctcttctc
    55741 ttttttgctt aaagaaatcc gttgtctttg aggccatcat cgaggatgac attcctgccg
    55801 cacaaataat caatgctttt ttcataaatc aatcactcct tttattttta taaaccaagc
    55861 atatcttgta agcgttttct attcaaaata tatatttccg tagcattgcg gaaaaaatgt
    55921 gtatcatatc ttgaataata atttctggca ccgtgatata ctagtgatag aattttttca
    55981 ggaggggatt tagtgagtat cacagtgaat attttgtata caagtacaaa tggacaagcc
    56041 agaaaatttg cagaagaaat ggtcgaaaga aacattgtca aagcgattcg tgcagaagaa
    56101 ggaaacgaga aatatgacta ttactttcca ctagaagatc cagaatctct tttactgatc
    56161 gaccgttgga aagatgaaga agcaatcgaa aagcatcaca aatccgaaat gatggctcaa
    56221 ataaacgaac tccgtaaaaa gtataaatta aaaatgaaag tcgaacgttt cactgattta
    56281 ccgtaaaaag atacacatgg aatgatagca atttatcatc catgtgtatc ttcacttttt
    56341 ccttattttt tattcgatct catctaatga ttcaaaccgt gtttttctct tatttttcgg
    56401 ataagtattt cagtcacccc aatatacatg attcctaaac ataccccaag gatattctca
    56461 aaaatgcgga tcgttactgc tcctggaacc ccgaataaac tggcagtgat tgccaaagca
    56521 ccaaaagaat taaaaactgt tttatatcta taggtagaac aaacacctaa agctagacca
    56581 cctaatatac cgacccagtt aaaaggaata aattggctga taccaataaa taaaacacat
    56641 ccaatcaatg agccaatgat tcggtctact gctcgttctt tgaaaccaat cgacatcaat
    56701 ccatagctag atacaataga agaaaatgca aaccctgccc acatcaatcg ctgaaaaggg
    56761 acatactctc ctgcaacgat caaaagacta atgccaaatg catagatgag catccaaatt
    56821 ttttcctgcg aatacatgcc tttcaaaaaa atctctttaa acaagctttt gtctttgttc
    56881 ttttctcgat gttttctgta aaggagaact gaaaaccaac aaaaaaacaa aaataataag
    56941 ctacttgttt gtgtaaagaa attcttgttc aacgaatctt taggtaacga ataaacaata
    57001 aagagatatg aaaagccgta caaagaagca ttccccattt tgggatcact agcggttgcc
    57061 attaatatgc tgcttagtaa aataaaatga atcaacatgc ccataactga ccatttgatc
    57121 agctggacat acggtgctac taatagtgag aacatgacga ttgctaaacc tatgatcgaa
    57181 tgggaaatct tataaccaaa gtcaacaaaa cgaatactca tcaacataca gaataaaacc
    57241 acagcgtatg gtttatgctt cgattcaaat aatgttgtaa ttataatgat aaaacaaatg
    57301 gcaaacaata cgatgaataa tgatcgtaaa aacaatgaac aaatatacat gcacttttct
    57361 tttttagaag ctgcttctcg tattttttgt tttaaaataa acggatctag ttgtagagac
    57421 tgataaaaat ccacctgttg tcgtcctttc tttttacttt tattttttac agtcatctat
    57481 gacaggttgt aaatactatg gaacatcata tggcgttgtc attttcttgt caactatttt
    57541 tcctattcat gtttttataa aaaaacatat cgttttcttg tatgattaaa tcaccacaaa
    57601 ataaacaact gagagaacga tatgctttat gaatggttct atcaaaaaaa ttctgagatc
    57661 aatagacaaa aatttgatga ttacagaggt ctcttacgaa accttacaga agaaaaaaac
    57721 attgatcgtt gctgcagttc tcttccctgc tccatgtgtc tgtagaaatt gtggttctgc
    57781 tgtggtagat ggaaatagga aattaatggt ggtgaaaaac aagaagaagg aaaccattgt
    57841 ccactttgaa taatataatc atatgccaat ggttatacgc ctaaaaaagc agcgctatac
    57901 ctgtaaaaac tgtcgaagcc attggaacgc tcaaagttat tttatccgtc caaggcactc
    57961 gatttcaaat catgtcagac ataaaatcac ttctttactg actgaaaaag tgtcgttatt
    58021 ttttatttcg aaaagttgtt aggtatcttt gacaaccgtt attcgcacat tgaaagagtt
    58081 gagaagctat ttaccaaagt cttccaaaag aattctgtct tgagtgttga gggttgtgaa
    58141 tttcgttctc acgtttccgt tgaggataaa ctgagtttta tttccgcgga tggcgagaca
    58201 gaaaaattaa tcgagaaaat ttacctacac aaaaaattcc tcgattaaca aactatttcc
    58261 ttagctgcac taatcctaaa gaaatagaat tcttagttac agacatgaac gccacctact
    58321 ttcagctcac caaacatgtt ctaccaaatg cgaaattggt gattgatcga ttacttggtt
    58381 ttcccacgcc tttcaaggag gcgtaacctt tattcacgag ttggttgaag ctttccgaga
    58441 caaaagaccc gacttgtttt tctccttatt gacaaagagc cattaaaact agagaaaaaa
    58501 ttacaactga tcgctgatta tttgtagaaa gaaaatcaaa gtattaccca ataaacttga
    58561 gtcattaggc aaaaaacact gcgaaactca agaatgattt tcgcagtgtt tttgtatatt
    58621 ttttattaac tagtattact acaaaggttt atttttcact tctcaacgca gcacaaaatt
    58681 gtctttactt atttactggt tgggttcaac cttctctact ccgatactaa tatttgtttg
    58741 tatacccctt ctaaaatcgg tagtttccat tcaattgatg ggtactagtt gattttaaaa
    58801 actatgctta ttcaattttt tattttagct catatatttc gtttctttct aaattttata
    58861 ctgatgaaaa caagtagcac acccaatatt gacaatataa actggctaat gatggtttct
    58921 ccagtttttg gaagatgctt atttccactg cttgttggat tgtttcctct tatatctgag
    58981 ctatcagcaa cttttctatt ctcttttgtt aaaaagatcg ccacttcttg ttctttttcg
    59041 atcttgaaag ttactggtgt cgcatccaat tcataccccg ttggcgcttg agtttctacc
    59101 agctgatatg ctcctggtgc taagccatct atcgccagtt ttccatctgc tcctgtggtt
    59161 agacctgact gcagaacggt tccattggca tcttgtaact caaaaacagc tccttgcagg
    59221 acttctccgc tttggtcatc gatctttgtc aacacgacac cgc
//
LOCUS       DASHHB010000012        51530 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.012, whole genome shotgun sequence.
ACCESSION   DASHHB010000012 DASHHB010000000
VERSION     DASHHB010000012.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 51530)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 51530)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..51530
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.012"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            153..1211
                     /locus_tag="U2K71_000818"
     CDS             153..1211
                     /locus_tag="U2K71_000818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AEC family transporter"
                     /protein_id="HEM7314924.1"
                     /translation="MNLFDIFRSTLTDMSIVSAITSTVFIILLGFVCRKKGIFSESVG
                     KILSKVVLTVALPALAFRAFMQDINTELLMQGINVLIWGLVIYIILILVSKPFFLSYK
                     GDKQTTLRVLTIFGSTTFFGTPIVSAIYGPVGVMYSSIFNIGYRIFLYSYGYIKMSGL
                     KMEAKNIKAMFVNPIIIATFAGLFIWLFQGYLPQVSVTGAEGEISQVAFLRIDQTAPW
                     LFQPMDYLAGLSSPLAWLAIGATLGEVSFKKAASEKTSWYYSLVKVVFVPALNIVILA
                     VLTATNILPVNFEALATIVIMMATPAATVAAAYAISFDREALLASNASLLSTIFAVVL
                     TPVWIVVLQMIHGIGLFS"
     gene            1215..2249
                     /locus_tag="U2K71_000819"
     CDS             1215..2249
                     /locus_tag="U2K71_000819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate dehydrogenase"
                     /protein_id="HEM7314925.1"
                     /translation="MEEESMRIACYGVRPNEIDFFEKLNIYHYDLSLYEELLTHDNIV
                     SAKDHDAVLLRGNCIADETNLAKMQEYGIRYVFTRTVGVNHIDLQAAADFGMTVARVP
                     SYSPNAIAELSLTFAMMLLRNTAYTTIRTSFKDFRVDEQMFSREIRNCTVGIIGTGRI
                     GLTEAVLFKGLGAKVIGYYIHQTEAAKKVLTFHSLEELLAKSDIVSIHVPHIPGENDQ
                     MINEGFLAQMKKGSILINTARGELQDNEAILRALKSNHLAGFATDVFANETEVFFRSF
                     KPWETIPDPAIQQLVEMYPRVLVTPHVGSNTDEALSNMISTSFENFREIIETGKTKNE
                     VSLPKARQLR"
     gene            complement(2351..3067)
                     /locus_tag="U2K71_000820"
     CDS             complement(2351..3067)
                     /locus_tag="U2K71_000820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEM7314926.1"
                     /translation="MMTNVLIIEDDPMVAELNRTFVESIPNMNVIESFRNVEEGKKYF
                     RQKNADLLLLDVYMPGESGIHFLSWLRQQGNDIPVILITAADDIETIKNVIRYGALDY
                     LIKPFTLQRFQLAIEKYLAFSQTIQTTEKADQKLIDQVFNHTTPIHEIPSFIDLPKGL
                     SKLTLKKISAFILKEDTIFSTKELAQKSSLSRISVKKYLNFLVEQDILNERIKYLEIG
                     RPLTCYELTKNGRKKLTDYC"
     gene            complement(3036..3821)
                     /locus_tag="U2K71_000821"
     CDS             complement(3036..3821)
                     /locus_tag="U2K71_000821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spo0B domain-containing protein"
                     /protein_id="HEM7314927.1"
                     /translation="MYLNGIETISSVTPLFSNDRFLGAVATLRNRSEVMKLMEELGGT
                     KQYINSMRAQTHEFMNKLHVISGLIDLKKYEEVTHFIQQLNQNYKKEIGQVSSLIKVP
                     AIAGFLLGKINEAKEQSVEMLLESDSFIPKMEMDDSIHSLLQVLGNLLDNAKEAVLQK
                     HERNGQVRIHLNYDTEAEVFIVTVCDDGVGISEEISEKIFTLGFSTKGINRGFGLNLI
                     HSIVKRHGGFLEFSKQTNGGTIVYLELPKKEDQNDDKCPDNRR"
     gene            complement(3812..4561)
                     /locus_tag="U2K71_000822"
     CDS             complement(3812..4561)
                     /locus_tag="U2K71_000822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEM7314928.1"
                     /translation="MNRSSVSDKISSIAKIVANDKLIQKKLQTGDPTHAIQGITNEIM
                     IASNLDFIVVMDPEGIRYSHADPSYIGLPFSSHEDVKKVIGGQEFFSEKEGMLGFGFR
                     YFVPIYYDQEIIGAVCVGLTNNTIDNQVKDGQKMIVFSLIIGLSVGIIGAVFLAQKMK
                     KVLLGLEPQEIAEQLSEKTIIENEVDEGILAISSDKTILLINTPAKNIFYQTEQLLDF
                     EVGKKSVIIYMTFCLKRRLSIKRPFGLNPCI"
     gene            4859..5383
                     /locus_tag="U2K71_000823"
     CDS             4859..5383
                     /locus_tag="U2K71_000823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HEM7314929.1"
                     /translation="MIGGIALETIYWYTLLICAGLAVLLIIFGDVFDFDGPLDPMLII
                     PWLTFTSLFGFLGERLTDQSSLFLFIASGGISIVLVFLLNFYILMPMKHAESTMSSSE
                     KTLEGQVAVVITPIPVRGMGEIQLKSVTGSISRPACFYSPQEQTAERGTKVLIIEIKE
                     RICYVMPYESMIKA"
     gene            5399..6898
                     /locus_tag="U2K71_000824"
     CDS             5399..6898
                     /locus_tag="U2K71_000824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flotillin family protein"
                     /protein_id="HEM7314930.1"
                     /translation="MEIPVVFIAIAIAALILLMLLIVFVAKYQTAKPDEALIISGSYL
                     GSKNVHIDESNNKIKIVRGGGAFVLPVFQRSNRISLLSSKLDVSTPEVYTEQGVPVMC
                     DGTSIIKIGSSVEEIATAAEQFLGKTREELENEAREVLEGHLRSILGSMTVEEIYQNR
                     DKFSQSVQEVASVDLAKMGLIIVSFTIKEVRDKNGYLDSLGKPRIAQVKRDADIAEAE
                     ALKETRIKKAQAEKESQAAELQRQTEIAESLKEKELKLATYKQEQDVAKAKADQAYNL
                     ESARAQQQVIEQEMQIKIVERQKQIELEEKEITRREKQYDSEVKKKADADRYAKEQEA
                     QAQKVKEVTEAEAERFRVEALAEAEANKTRLAGQAEAEAALAKGKAEAEAKQKIANAF
                     KEYGEAAVLSMVIDMLPQLMREAAQPLGNIEKISVVDTGSSSGETSGANRVTNYATNL
                     LSSTQETLKATTGLDLKEIVENLSTRGTTSQVNMYEAPKEAEQNSMKEE"
     gene            complement(6986..7588)
                     /locus_tag="U2K71_000825"
     CDS             complement(6986..7588)
                     /locus_tag="U2K71_000825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM7314931.1"
                     /translation="MQFGRFRLGMRTIKSALAVFLCILFFHLTQRGIPMIAALSAVFS
                     LRQDLTTTVSFGKSRIIGNTLGGTLAIIYFFVKSYFHNDFLVELILLPILVIIVIVVS
                     DGINNNSGIISAIATLLLIALSIPQGESALYAIQRVLDTFIGTFIAIGINFFLRPPET
                     EKKEELSEDLVELQKKERLLRTNLQEIEAKIQKQKKNSED"
     gene            7692..8648
                     /locus_tag="U2K71_000826"
     CDS             7692..8648
                     /locus_tag="U2K71_000826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic acid exporter family protein"
                     /protein_id="HEM7314932.1"
                     /translation="MRIGMRTVKTVISVFFSMVTAGSLSLLYWPAAGIIALLSVGNTK
                     RSTLRTGMNRIAAFILATATALFSFFAVGYTIFGFSLFLFLFIPLASRFKLTEGIVVN
                     SVLVTHYLVEENMSWQLIGNEAGLMAIGLVFALLANVYMPDRTKQLKENQIQIEKEFR
                     NILRQMAEFLLSENKEDVQIKCEHLLTFIRESQEDAREHQENYWLRQPLYYETYFSMR
                     RAQANVIKDMLENLERIQQPAYYGKHIYGLLIYTAETFSESNDGRQILTRIEEVYVLY
                     RQMPLPTSRPEFEDRAELFQFLQSFKSFIEIKAEFSQQKIQK"
     gene            complement(8953..9909)
                     /locus_tag="U2K71_000827"
     CDS             complement(8953..9909)
                     /locus_tag="U2K71_000827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM7314933.1"
                     /translation="MKKYQWGIVGLGTIAHEFAESFNQETSELTAVASRTSEKAENFA
                     HRYNIPKAYGSYQEMLDDAEIDIVYIAVPNRQHIDHILAALEAGKHVLCEKAITMNKK
                     ELADAMRLAEEKNLILAEAMTIFNMPLYQQLRSIMDTGKLGALKMIQAPFGSYKEPDP
                     KNRFFNPELAGGALLDIGTYAVSFARFFLSSQPEVVASTMVPFETGVDEQSVTILRNK
                     ENELAAVSLTFQAKMPKVGIVAFENGYVTIADYPRADRAEILFTDGTKEFIESGNTSE
                     AMNYEIKNMVHMIEGDLPNRSLFLTKDVIEILDQMQVLWKNM"
     gene            10083..10643
                     /locus_tag="U2K71_000828"
     CDS             10083..10643
                     /locus_tag="U2K71_000828"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314934.1"
                     /translation="MHQVYIDLIMSVLLEQFETEKNFLDEYLLISPEQWQKWKDGEKN
                     LPSEAMQKVKNLFTDYEWMLTQKILRQTIIFPEKRNVAVAEYKQIKTRIAQKWLSSKA
                     AKVELIPLKNNQQAGRYLDLKVSIQYDEWGYDDILNFRLPADVQKQIEGEKIELLDWV
                     NENLEETYVHEANGEREEQRKDFSSE"
     gene            10636..11460
                     /locus_tag="U2K71_000829"
     CDS             10636..11460
                     /locus_tag="U2K71_000829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M15 family metallopeptidase"
                     /protein_id="HEM7314935.1"
                     /translation="MNKFLGFGAVVIMIAAMGYVIFSDDDSHVQAGNQAVERTSTKTT
                     ASKEKNSQTTSSKVKDKETKDRTSKQLPDVKTDDWKLVLVGPDHKLEKEIDESTQLAS
                     LSNGYLIDKRIAPEYEAFAKAAEAAGFPLVMVSAYRSVSSQQQVFEQNVQDVMSRQGV
                     SEEEATKITKETITEPGYSEHHTGLAVDVVDQNWYNSYPSTVLDASYGDQPGAKWIAD
                     NAAQYGFIVRYPKGHEDITKITYEPWHLRYVGKENAEYITKHQLTLEEYLNELNEK"
     gene            11470..12087
                     /locus_tag="U2K71_000830"
     CDS             11470..12087
                     /locus_tag="U2K71_000830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEM7314936.1"
                     /translation="MAKKKWKKQKRKNIRWPAVVAGVAVILLAFVFSLKNLSSPFTDN
                     QQENEAQSHQQFIERLVPHAQELQDGYGVLPSIILGQAILESNWGKSTLASKYNNLFG
                     IKAYGDQKKVSLETKEFVNEEWITIQGDFKVYDSWEQSMDDHTQLFVQGVDWNPALYE
                     KVITATNYQEAAQALQDAGYATDPGYAQKIIQVIETYQLNQYDRR"
     gene            12105..12788
                     /locus_tag="U2K71_000831"
     CDS             12105..12788
                     /locus_tag="U2K71_000831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEM7314937.1"
                     /translation="MAEQFTPEIMTELRKDIVRVPEIILEASGIKIFGKLIRSIIFTT
                     DIAIIRNTNANAVIAVYPFTPHPAITKSIIEAADIPVFSGVGGGLTQGARSSYMSMFA
                     EAQGSLGVVLNGPTPLETVKEVCRVVDIPVISTVTSKYTEIDEKLAAGISIINISAGK
                     ETAEAVRHFRKKYPELPIIATGGPTEESIAETIQAGANAITYTPPSSGELFSRKMEKY
                     RETEKKNHE"
     gene            13471..14754
                     /locus_tag="U2K71_000832"
     CDS             13471..14754
                     /locus_tag="U2K71_000832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM7314938.1"
                     /translation="MKKRMRIILALSVMLFFLTACGQEDDKKITLNMAWWGSQVRHDA
                     TVKVIELYEEKNPHVTIDYEFYDYEGYYTKLGTLAASDDIWDIFQLNNQFPQYINQIE
                     PLDEYVEQGIIDISNADESYLATTTYDGQLVGLSNGVNSFAIAYNKNIFEQLNIPEPA
                     SNWTWDEFEQISYQITEELGIYAISRFEDFIAGCIIQIPQVEKGLNFYNREDLSNSLG
                     FENPDYLTDFFQLRKNLVDKGAHPEPGGGAASTTDLSFQAVRDREAAMVFLSSNQLTE
                     ILSGAPEDMEIQLINPPRRKADGESGIPLRSSQMLSMAKSSAHKEEAAKFIDFFQNSE
                     EANEILKGERGVPIMSNIREQLARADDATLQATFDYLEMISDMDNGEVNVFDSALNPE
                     IQDQYNLLVEKVIYEEVTAEEAAESLYEFASNILG"
     gene            14770..15657
                     /locus_tag="U2K71_000833"
     CDS             14770..15657
                     /locus_tag="U2K71_000833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7314939.1"
                     /translation="MKKEKLKRFLDQDNVVGYVFASPFILGFLLITVIPMILSLYYSF
                     TDYRLGNPVSWIGLDNYIKMLSDARFLNSIRVTFLYVFASVPVKLIFGLLIAYLLTRN
                     IKGTTFFRSLYYVPSLIGGSISIALVWRELFSKNGLVNIFLERLGAPALSWFSDQKLV
                     MIPLVLMSAWQFGSAMIIFAAGIKEIPGSYYEAAEIDGANKWQTFYKITLPLLSPIIL
                     YNLVMQTIAAFMTFTQAFIITQGGPNDATNFYALYVYNQAFSFNSMGYASAISWIMLL
                     IMGAVTFLIFKSSKKWVVV"
     gene            15674..16510
                     /locus_tag="U2K71_000834"
     CDS             15674..16510
                     /locus_tag="U2K71_000834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM7314940.1"
                     /translation="MNNTVTKWFQYGFILLLGLVMIYPVLWMIAGSFKTDQEIMSGSL
                     NLIPETFQWENYAKGWAGFAGISFFTFFRNSFVISAVSTIGTVLSSTCIAYALSRIDF
                     KGKRFWFVVMLVTMMIPAQVILIPQFIIYNRLNLVGTYVPLILPHFFGQAFFIYQIMQ
                     FMVSIPKELDESAIIDGCSKYSVFTKIIFPLLKPSIITTIIIQFYWKWDDFMGPLIYL
                     NKPRSYTVSIAIKLFADAGSSTTYASMFAMSTLSLLPVFLIFLFFNKYLVQGISTSGL
                     KG"
     gene            16513..17136
                     /locus_tag="U2K71_000835"
     CDS             16513..17136
                     /locus_tag="U2K71_000835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314941.1"
                     /translation="MKKPIEEMFLYKLNYYVCRIFLTSLISNALFALVLGCLFTLGLV
                     LSARVVYLIYFLMMYPSYYLSVHYLAEKKVCWRKGKIKRMCFIITLFQLFVIIFAVDW
                     YLLVGKIGAWYLIPFLLCLLLFVSHSFFSTLIDICKEDKADSLVYLIKRNIWLLSKNV
                     KGFLWYLCLLYGLFWCIQHDFLLTALLYPGLPFVTVFCLKEKENDFK"
     gene            17133..18425
                     /locus_tag="U2K71_000836"
     CDS             17133..18425
                     /locus_tag="U2K71_000836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM7314942.1"
                     /translation="MKKKYVQVGLGGRARFFYEAIASTYHQTCELVGFCDVSQTRMDY
                     ANRILVNKYGQKKVKTCMPSQFEQLLDDVRPAEVIITSVDRTHHDYIIRAMKKGYDVI
                     CEKPLTIDEEKAQEIITVQKQTGRSLRVTFNYRYAPYHTKIKELIESDVIGDVFSIHF
                     EWLLNTQHGADYFRRWHRNKHNSGGLLVHKSTHHFDLVNFWLNSRPQKVFAFGGLNFY
                     GMHNAEKRGVEIFYSRCYGSELAKADPFAIDLSANEELREMYLEAEKDSGYIRDRSVF
                     SDDISIEDTLGVTVKYETGAILTYSLNAYMPWEGFNVSFNGSKGRIEMKVIEKSYINA
                     GGDKKDEGAAVLQSIHVYPMFGEPYEVIVEQEAGGHGGGDPRLLDDLFGEEKEEDPWN
                     RAATHVDGILSILTGIAANHSIASGKAVVIDELVSLKE"
     regulatory      18784..18881
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00167"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="purine riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="guanine and/or adenine"
                     /db_xref="RFAM:RF00167"
     gene            18997..19578
                     /locus_tag="U2K71_000837"
     CDS             18997..19578
                     /locus_tag="U2K71_000837"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HEM7314943.1"
                     /translation="MKELVERIQKDGRILGEGVLKVDSFVTHQVDPVLMEQMGKRFAE
                     VFKEQGITKVVTIEASGIAPALFAARELDVPMIFARKAKSLTMDEELLTASVYSFTKQ
                     VTSTISISRKFLSSEDKVLIIDDFLANGQAAKGLIELCQQAGAQVEGIGIVIEKSFQD
                     GRQLLEEMGLRVVSLARIASLTNGQVEFLEEDA"
     gene            19581..20888
                     /locus_tag="U2K71_000838"
     CDS             19581..20888
                     /locus_tag="U2K71_000838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine permease"
                     /protein_id="HEM7314944.1"
                     /translation="MEKETQNGKAAVLGLQHLLAMYAGAVAVPLLIGTGLGFNQEQMT
                     YLISIDIFMCGLATLLQLTVNRFFGIGLPVVLGCAIQAVAPLILIGTNEGVGAIYGSI
                     IASGIFVVLVSGVFSKIKKLFPPIVTGTVITVIGLTLIPVAIEKMGGGNATAADFGDK
                     TNLLLAFVTILLIVGVQMLAKGFIRSIAVLIGLVGGSILAAFMGMIDLSAIGDAPLFH
                     VPQPFYFGKPTFDVWSILLMIIISMVSMVESTGVYFALGDITGKNVGEEELKKGYRAE
                     GLAVILGGIFNTFPYTGFSQNVGLVQLSGIKTRKPIYFSAFFLIVLGLFPKIGALAQI
                     IPEPVLGGGMLVMFGMVAVQGIRMLLEVDFSNDKNLLIVAVSVGFGLGFNVTPTLFSQ
                     MPQTLQMFTGNGIVMSSITAIILNLIFNGLKKDEEILEEAFKK"
     gene            21304..21792
                     /gene="purE"
                     /locus_tag="U2K71_000839"
     CDS             21304..21792
                     /gene="purE"
                     /locus_tag="U2K71_000839"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HEM7314945.1"
                     /translation="MTAKISVIMGSTSDWETMKEACQILDEFGVSYEKKVVSAHRTPD
                     LMFEYAQNARKKGIKIIIAGAGGAAHLPGMVASKTTLPVIGVPVQSRTLNGLDSLLSI
                     VQMPGGVPVATTAIGKAGAINAGLLAIQMLSMYDLELEAKLAERRSMLAKTVIESSDQ
                     LG"
     gene            21785..22909
                     /gene="purK"
                     /locus_tag="U2K71_000840"
     CDS             21785..22909
                     /gene="purK"
                     /locus_tag="U2K71_000840"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HEM7314946.1"
                     /translation="MVKPLLPGSTIGIVGGGQLGRMMAISAKEMGFKVGVLDPVSDCP
                     AAQVADWHIESVYDDTFALKELARRTDVITYEFENVSVEALNAILPMSFIPQGTDLLA
                     ITQDRLLEKSFLETNNIVIAPYATIVSPTDIQDAIDGIGYPCVLKTTRGGYDGKGQYV
                     LKSRADLAPAMDLLREGTCVLEAWIPFEKEISIMVAGNGQGDFTTFPVVENIHHNNIL
                     HETIAPAAIDQDVIEEAERIARVIAEAVSLSGVLGVEMFLTKTGGLYVNELAPRPHNS
                     GHYSIEACSMSQFDAHIRGICGWPLGEKVRLISNAVMVNVLGEQLLESYRLIENKPDW
                     QFHFYGKKQAKKGRKMGHITILTEDIQQTLEEIKQTNIWD"
     gene            22922..24217
                     /gene="purB"
                     /locus_tag="U2K71_000841"
     CDS             22922..24217
                     /gene="purB"
                     /locus_tag="U2K71_000841"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HEM7314947.1"
                     /translation="MLERYTRPEMGNIWTEENKYRAWLEVEILADEAWAELGEIPQED
                     VKKIREHASFDIDRILEIEKLTRHDVVAFTRAVSETLGEERKWVHYGLTSTDVVDTAY
                     GYLIKQANDILREDLKRFTEIIGEKAKEHKYTVMMGRTHGVHAEPTTFGLKLATWYSE
                     MKRNIERFEHAAKGVEAGKISGAVGTFANIPPFIEEYVCKQLGIRPQEISTQVLPRDL
                     HAEYFSAMALIATSIERFATEIRGLQKSETREVEEFFAKGQKGSSAMPHKRNPIGSEN
                     MAGLARVVRGHMVTAYENVTLWHERDISHSSAERIILPDTTILLNYMLNRFGNIVKNL
                     TVFPENMKRNMSATFGLIYSQRVMLKLIDKGMTREEAYDLVQPKTAYAWDNQVQFRPL
                     LEEDPTITSVLSKEEIDDAFDYHHHLKNVDVIFKRVGLE"
     gene            complement(24339..25727)
                     /gene="ltrA"
                     /locus_tag="U2K71_000842"
     CDS             complement(24339..25727)
                     /gene="ltrA"
                     /locus_tag="U2K71_000842"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM7314948.1"
                     /translation="MRRPQKTRKLGYHQRASVEHEGYDEVRSTSCGEQRKQDGVENLF
                     GRILDRNNLNQAYLQVVRNKGAAGADGMTVDQLLPYLKEHREELLSNLRQGNYKPQPV
                     LRVEIPKPNGGVRKLGIPTVIDRMLQQAINQVLQPLFEPEFSDNSYGFRPNRSAHDAV
                     KKAKSYYEQGYRYVVDIDMKAYFDTVNHDKLMYFIERKVKDKQVLKLIRQYLMSGVME
                     NGLITTTEEGTPQGGNLSPLLSNIYLNEFDQVLENLGHKFVRYADDCNIYVRSKRAGQ
                     RVMKKSIQFLEEQLKLTVNREKSAVGSPLKRKFLGFCILPTKKGIKIRPHAKAKQRVK
                     SKLKQLTKRNRGRSIQLILKEIKQLMTGWINYYGIGEMKEFMRELNGWLKRRIRQYIW
                     KQWKNPRTKRKNLIRLGIDKAKAYEWSNTRKGIWSVSKSHILHRSLTDKELARQGYED
                     ISLKYQFVHSTY"
     gene            complement(26296..26673)
                     /locus_tag="U2K71_000843"
     CDS             complement(26296..26673)
                     /locus_tag="U2K71_000843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314949.1"
                     /translation="MEDAIKQGQLFLSELTAEERSCLYNDLLSIKDVYLCNKDTSFYT
                     FRKIRRATKRCAECRKRTFLYQTEHKGELSISQIHEQKRFKNHYRIQILFNGQEMEYA
                     FLENLLQHLHNQHFSYNSQAIYA"
     gene            26929..27657
                     /locus_tag="U2K71_000844"
     CDS             26929..27657
                     /locus_tag="U2K71_000844"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HEM7314950.1"
                     /translation="MEKKELLYEGKAKKIYLTSDENLIWIEYLDQATALNGARKDQIS
                     GKGELNNQITSAIFRYLTQRGIKNHFIEQLSKTEQLAKKVQIIPLEVVIRNIAAGSFS
                     KRLGVPEGKEFIKPIIEFYYKDDQLDDPFINEEHIYFLQLAKPEEISELKAQALAINE
                     ILTELFAKVGLVLVDFKLEFGRSNGQILLADEVTPDTCRLWDERTNEHMDKDVYRRDL
                     GDLIPVYQEVYNRLSELTEEKERV"
     gene            27657..27911
                     /gene="purS"
                     /locus_tag="U2K71_000845"
     CDS             27657..27911
                     /gene="purS"
                     /locus_tag="U2K71_000845"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HEM7314951.1"
                     /translation="MYFVKVYVTYKDSVLDPQGEAVKKAVHRMGYETIEDIRIGKYFE
                     IQLADAEEPIDTVIETICDKLLANVNMETYRYEIKKMEEV"
     gene            27913..28584
                     /gene="purQ"
                     /locus_tag="U2K71_000846"
     CDS             27913..28584
                     /gene="purQ"
                     /locus_tag="U2K71_000846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HEM7314952.1"
                     /translation="MKFAVIVFPGSNCDADMLWAVREIMGADAEFVRHDESSLEGFDG
                     VLLPGGFSYGDYLRCGAIARFSTIMEEVIRFAEEGKPVFGTCNGFQILTEAGLLPGVL
                     RRNESLHFICKTVPLKVNPKTAFTSEYEADEIIYLPVAHGEGNYFCDEETLQELKKNN
                     QIAFTYNDNINGSVENIAGITNKAGNVLGMMPHPERAVEDLLGSEDGKRFFASILKNF
                     GKVTV"
     gene            28585..30807
                     /gene="purL"
                     /locus_tag="U2K71_000847"
     CDS             28585..30807
                     /gene="purL"
                     /locus_tag="U2K71_000847"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HEM7314953.1"
                     /translation="MKTLKEPTAEEIKKKRIYAEWGLTDEEYRMIEEDILGRMPNYTE
                     TGLFSVMWSEHCSYKNSKPVLRKFPTSGPHVLQGPGEGAGIVDIGDGLAVVFKAESHN
                     HPSAVEPYEGAATGVGGIIRDIFSMGARPIALLDSLRFGELDNPRTKYLLEEVVAGIG
                     GYGNCIGIPTVGGEIAFDPCYEGNPLVNAMCVGLIEHKHIQKGQAAGVGNSIMYVGAK
                     TGRDGIHGATFASEEFNQEEEQQRSAVQVGDPFMEKLLLEACLELILHHSDSLIGIQD
                     MGAAGLVSSSAEMASKAGSGLILTLDEVPQRETGMTPYEMMLSESQERMLICVKSGEE
                     EKIQELFQKYDLDAVTIGKVTDDGQYRLYHQGEEVANLPVDALAEDAPVYHKEMKEPA
                     RIVEFQQLAPYQPVIEEPGQVLLDLLQQPTIASKRSIYETYDSQVQTNTVVRPGSDAA
                     VMRVRGTNKALAMTTDCNARYLYLDPKIGGQIAVAEAARNIIASGGKPLAITDCLNYG
                     SPDKPEVFWELSTSADGIAAACETLGTPVISGNVSLYNETDGQAIYPTPMIGMVGLIE
                     DHKHITTQEFKKSGDLIYILGKTFADFDGSELQKMQLGRIEGVIRNFDLSIEKRNQEL
                     VLTAIQNGLIESAHDCSEGGLAIALAESAFKHQLGISVQFELSSAQLFAETQSRFVLT
                     VAPENKTRFEEMMGDAAVLAGKVTDEAIIEISATDGQIKIETAVARKCWEDAIVCLMK
                     "
     gene            30792..32231
                     /locus_tag="U2K71_000848"
     CDS             30792..32231
                     /locus_tag="U2K71_000848"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735680.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HEM7314954.1"
                     /translation="MSYEVKSLNEECGIFGVWGHPDAASVAYFGLHSLQHRGQEGAGI
                     VSNDRGKLKGHRDLGLLSEVFKDQRKLQQLTGEAAIGHVRYATAGNGSVDNIQPFLFK
                     FHDQSIGLAHNGNLINARSLRKELEENGAIFHSNSDTEILMHLIRRSEETDFLNKLKS
                     ALNEVKGGFAYLLMTETAMIAALDPNGFRPLAIGQMKNGAYVIASETCALEVIGARFI
                     RDVQPGEIVIINDEGIQIDCFTKDTQLSICSMEYIYFARPDSNIAGINVHTARKNMGR
                     NLAKESPVKADMVVGVPNSSLSAASGYAEASGIPYEIGLVKNQYIARTFIQPTPELRE
                     QGVRMKLSAVRGVVEGKKVIMVDDSIVRGTTSRRIVHLLKEAGAKEVHVRIASPPLKY
                     PCFYGIDIQTRKELIAANHTIDEIKECIGADSLGFLSEEGLIESIGLEKDAPYSGLCM
                     AYFNGDYPTPLYDYEEKYQESLKEKVSFF"
     gene            32254..33306
                     /locus_tag="U2K71_000849"
     CDS             32254..33306
                     /locus_tag="U2K71_000849"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HEM7314955.1"
                     /translation="MNSMANAYAKAGVDVEAGYEVVERIKKHVKKTERLGMMGAIGGF
                     GGCFDLSVFQLKEPVLVSGTDGVGTKLAVAIEADKHDTIGIDCVAMCVNDILAQGAEP
                     LYFLDYLATGKNDPLRLENVVKGVADGCVQGNMALIGGETAEMPGMYSGNDYDLAGFA
                     VGIAEKSQLLTGETINPGDVLLGIASSGIHSNGYSLVRKIFFEQQQFSLSSKLPELKK
                     TLGEELLEPTKIYVSSLLPLIKEKKIKGAAHITGGGFVENIPRMLPESMAAQIKLGSW
                     PILPIFGCLQKYGEIPEMEMYEIFNMGIGMVLAVQESKVKEIVHEIEQAGEKVYEIGK
                     VIAKEKHRVVFIGEER"
     gene            33303..33881
                     /gene="purN"
                     /locus_tag="U2K71_000850"
     CDS             33303..33881
                     /gene="purN"
                     /locus_tag="U2K71_000850"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HEM7314956.1"
                     /translation="MRIAVFASGNGSNFQALADYLSKKGLESSIDWLFCDQPEAYVLK
                     RATALSVPADCFSPKEFDSKKEYEEAILHKLKEKKIDLIVLAGYMRIIGPVLLKNYDK
                     RIINIHPSLLPAFPGLHGIRDAFEAGVKETGVTIHYIDQGVDTGPIIRQEKVRIEQED
                     TFDSLEEKIHRVEHRIYPEVISEIIENGGYLK"
     gene            33878..35413
                     /gene="purH"
                     /locus_tag="U2K71_000851"
     CDS             33878..35413
                     /gene="purH"
                     /locus_tag="U2K71_000851"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HEM7314957.1"
                     /translation="MTRALISVSDKTGIVAFAQGLIEQGVEIISTGGTKHALDKAGIK
                     TLSIEEVTGFPEMMDGRVKTLHPKIHGGLLGRRDLPSHMEAMETENIQPIDFVCVNLY
                     PFKETIMKPDVEIAEAIENIDIGGPSMLRSAAKNHEFVTSVVDPSDYEEVLSELRQYG
                     HTSLSFRQKLAAKVFRHTAAYDALIAQYLTDWVEEKEPEKLTLTYERKQTLRYGENSH
                     QAAAFYQSALLEKYSIAAAKQLHGKELSYNNIRDADAALRIMKEYDEPTVVALKHMNP
                     CGIGTASTILEAWEAAYEADPVSIFGGIIVLNREVDLPTAEQMHKLFLEIIIAPSYTQ
                     EALELLQKKKNIRLLTVDFAQEENQNREETVSVLGGLLVQDQDLAIENEEEWKVVTKR
                     KPTPAEAKALAFAWRAVKHVKSNAIVLANERQTVGIGAGQMNRVGSVKIAIEQAQEKM
                     EGAVLASDAYFPMDDSVEYAAKHGIKAIIQPGGSIKDQDSIDMANKYDLAMVFTDVRH
                     FRH"
     gene            35429..36703
                     /gene="purD"
                     /locus_tag="U2K71_000852"
     CDS             35429..36703
                     /gene="purD"
                     /locus_tag="U2K71_000852"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HEM7314958.1"
                     /translation="MNILVIGAGGREHAISKKLLASPKVDTVYCAPGNPGMTKDGINC
                     VAISERDHSALIHFVKKEAIDWSIVGPEVPLLNGIVDDFMAEGLKIFGPTKAAALIEG
                     SKSFAKQLMDTYQIPTAKSRSFECYEKAAAYIKENGAPIVVKADGLAAGKGVVVATTV
                     KEALDAAEQMLNQHRFGDSSKKIVVEEFLAGEEFSLLAFVRNTKVYPMVISQDHKRAF
                     DNDKGPNTGGMGAYSPVPQISKKMIEKAVEEVLKPAALGMKELGRSFTGVLYAGLIAT
                     KEGPKVIEFNARFGDPETQVVLSRLKSDFAQVIDDLLENRTVELKWQQEGYNVGVVVA
                     AAGYPEEYETGMVLPDFLEEELSVYYAGVSSNNGKLVGAGGRLYLVEAYGETIKEAQK
                     KIYTALMDKTTDGTFYRTDIGEKAANSELIVK"
     gene            36825..37739
                     /locus_tag="U2K71_000853"
     CDS             36825..37739
                     /locus_tag="U2K71_000853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEM7314959.1"
                     /translation="MKKIQLGTSDQKAAAVILGCMRINNAEDPVKVIETAYENGIDFF
                     DHADIYGNGECEEIFADALAKTSIKREDLFIQTKCGIVPGVMFDFSKKHIIESVEKSL
                     TRLKMDYVDALLLHRPDTLVEPEEVAEAFDELERSGKVRYFGVSNQKPMQIELLKKYV
                     RQPLVANQLQFGLKHTGMIDQGIHVNMTDEASIDHDGSVLDYSRLHDMTIQAWSPYQY
                     GFFEGVFIGNEKFPELNQTLEELAAKYQTTPTGLASAWILRHPANMQVIAGTMNVGRI
                     KEIAQASEINMSREDWYELYRAAGNILP"
     gene            complement(37872..39437)
                     /locus_tag="U2K71_000854"
     CDS             complement(37872..39437)
                     /locus_tag="U2K71_000854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEM7314960.1"
                     /translation="MYKVFIIEDEYLIRNNLREQITVLGSYYPITYVGEAGDGEMALS
                     SIIDLQPDIILTDIQMPFMDGLTFSKEARKLLPWTRLIFISGFDDFDYMKGAIQVQAD
                     DYLLKPIKSSELKQAIEKAVTALDLQQKETHTSSDLVFELKKNHFLNGLFKGSLTISE
                     TLKLAESFNRSLAGKKMTVLLAANQVSTDFEDYAHLSDYLKFLFRENQQVIFSAVSSR
                     FIKFLIVDSLEENVIETSYQVAQTLVHELEKGQKNNFSVAIGPVAERISELPASFEIT
                     QMILETASSLKEQIISYEDLIQEAKLPVDSVFYFDLSLDLSSLSKNEVSKYIEHLTKP
                     QGTPTRTRLYRLFVLTKLLQHVCLKEKKIPQEMVHCTSTSQRLAISADDSLYRSTVYM
                     FISFLTALPSQPSRNKYQPILNHALDFIKENFTDPDMSLTWVAQQVALSPSHFSTIFS
                     QSFNQTFIDYLTEQRIELAKKLLQQSDYRISDIAFEIGYNDPNYFSYLFKKKQGISPK
                     EYRTQLFLKNKNN"
     gene            complement(39430..40881)
                     /locus_tag="U2K71_000855"
     CDS             complement(39430..40881)
                     /locus_tag="U2K71_000855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM7314961.1"
                     /translation="MTKNLSIKQIFAQANKAMLLFAVIPLLLSILLYSQQLLIYQQTI
                     ANIQKADAIALKVDQKVLESMWDLISGQVTVDEFNEENILEELRNDIREIKNNTNTLK
                     EISTLDVSLRTIDTLEDYQNELINNILAKDFMDTNEEIINQVDSVTQLLSDILQDFVR
                     VEIDLASKKNTTLVRSLILLTIVELGVLSTIFFYAKKNRRFLERKVQEPINQLIEMSE
                     QLSSGNLGFRLTLPETLELQTLTASMNKMAEDLNQSLRENALKQYHLAQSEVRVLQAQ
                     ITPHFIYNSLDAIISLIEQDCFEEALTMTHALSGFFRTSLSKGQDWIPLEKEISHIND
                     YLVILKIRYGHMLDYKIDIPETLFPVSILKMILQPLIENAVYHGTKYVRHKGFIRITT
                     CKIDEMIQFKVIDNGIGIPAEKLAEIRKELAKGIDSDFSIGYGLYNVNKRLLLYYGND
                     QKPITIDSKYREGTTVTLMIPLSPKTIGGNKHV"
     gene            complement(40895..41911)
                     /locus_tag="U2K71_000856"
     CDS             complement(40895..41911)
                     /locus_tag="U2K71_000856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM7314962.1"
                     /translation="MKRVLFFLALVVIAYQMMGCTPNTKEAPDPLVVGFSQSGTESSW
                     RKKHTESIITALEKEDYQVLYRNGYMNQERQIQDIRTFIAYKVDMIIFTPLQESGWDS
                     VLKEAKRAGIPVILVDRHIQTNDPELFVTHIGPSFKAEGNRAGLFVSNHFTNSKKQEI
                     RILELAGSENSTPTKLRSEGFLESINRKPTLKKDGTIDHKPPMTIVHRIVGDFIRMKG
                     KDQMKRYLETNDLSEIDVLYSHSDEMTHGALAAIKETSIKPGEDLIIVTIDGQEDMIE
                     KLRSGIVNCVVECKPDVGWYVANTVTRYFGNIQTGKTLPKDIFISETVFSQQNIANIP
                     TRNY"
     gene            42131..43219
                     /locus_tag="U2K71_000857"
     CDS             42131..43219
                     /locus_tag="U2K71_000857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HEM7314963.1"
                     /translation="MKNKFRKIVGTIAILTASILLSACGNSGSADDSTGYVGIAMPTK
                     SAERWIADGNNMVSELEKLGYKTDLQYGEDKVENQVAQIENMITKGVDTLVIASIDGS
                     ALTDVLAKAKEADIKVIAYDRLLMNSENVDYYATFDNFGVGVSQAAYIEEHLGLKEGK
                     GPFTIELFGGSPDDNNALINYNGVMSVLQPYMDNGQLVVPSGQTSFSQIATLRWDGST
                     AQARMDNLLSANYTDQTLDAVLSPYDPISLGIISSLKGVGYGSESKPLPVITGQDATV
                     AGVKSIIAGEQTQTIFKDTRILAKNTIEMIKAISDGEEVPVNDTETYDNGVKTVPTYL
                     ANTVSVDKDNYQAELIDTDYYKESDLKN"
     gene            43273..44799
                     /locus_tag="U2K71_000858"
     CDS             43273..44799
                     /locus_tag="U2K71_000858"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HEM7314964.1"
                     /translation="MADCILKMADITKKFSGIRALDCVNLMIERGEIHALCGENGAGK
                     STLMNVLSGLYPYGSYDGEIFYDGKLCQFKDLKDSEEKGIVIIHQELALSPYLSIKEN
                     IFLGNEQAERGVIDWELTEKKTYELLNKVGLSIDPNLLVSQIGVGQQQLVEIAKALSK
                     SVRLLILDEPTAALNEEESANLLRLIRELKEQGVTSIIISHKLNEIVDIADRITILRD
                     GQTIETLENEMISEEHIIRGMVGRDLVDRYPERKPDIGEVYFKVEDWTVYHPLDHERI
                     INDHINLHLKKGEIVGIAGLMGAGRTEFAMSLFGHSYGSKISGRILKEGAEISVKTVP
                     SAIANGLAYVSEDRKSLGLNLLMDIRENVTLSSLDKISQKGVLDKEKEVTEAENFRKK
                     MRIKTNSIHQIVSSLSGGNQQKVVLSKWLMTQPDILFLDEPTRGIDVGAKYEIYTIIE
                     EMAANGKAVCLISSELPEILGMCDRIYTMNEGRITGEVSREDADQEVLMKLMTKEEVG
                     "
     gene            44801..46006
                     /locus_tag="U2K71_000859"
     CDS             44801..46006
                     /locus_tag="U2K71_000859"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7314965.1"
                     /translation="MEQAKNLTDKKEKKLNLKSRAADLFGRYSMVIILAGLLIAFQLM
                     TDGIFWRPLNITNLVLQNSHILVLAAGMLLVVLLGHVDLSVGSVMAFVGAIAGMMMVN
                     RQMNPWMTVIACLAVGGLIGAWQGFWVAYAGIPAFIVTLAGLLMFRGLTQVVLGGQSL
                     APFPAVFQKMSTGYLPDLSEGSSLHFLTLILGMIITIVLVVGQWRTRNRRKNHLFEVE
                     SPTAFLVKAVFTAVVVLGITYIFASYQGYPIILIILGLIVAAYGFLTNKTVAGRQIYA
                     TGGNRKAAELSGIKTKRITFWVFVNMGVMAALAGLVLAARLNAATPQAGTSMELDAMA
                     AVYFGGASTSGGIGTIMGAIVGGLVMGVLNNGMSILGVGVDWQQAIKGLILLLAVVLD
                     LYNKKKRTT"
     gene            46263..47240
                     /gene="guaC"
                     /locus_tag="U2K71_000860"
     CDS             46263..47240
                     /gene="guaC"
                     /locus_tag="U2K71_000860"
                     /EC_number="1.7.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GMP reductase"
                     /protein_id="HEM7314966.1"
                     /translation="MKVFDYEDIQLIPNKCIVNSRSECDTTVTLGKHTFKMPVVPANM
                     QTIIDETIAEFLAENGYFYIMHRFDEAARIPFIKKMKKRGLITSISVGVKKEEYSFIE
                     KLAEESLNPDYITIDIAHGHANSVIDMIQHIKKYLPETFVIAGNVGTPEAVRELENAG
                     ADATKVGIGPGKVCITKIKTGFGTGGWQLAALRWCAKAARKPIIADGGIRTHGDIAKS
                     VRFGATMVMIGSLFAGHEESPGETKVENGIVYKEYFGSASEFQKGEKRNVEGKKIWIQ
                     HKGSLKDTLVEMQQDLQSSISYAGGRDLEAIRKVDYVIVKNSIFNGDTI"
     gene            47504..48928
                     /locus_tag="U2K71_000861"
     CDS             47504..48928
                     /locus_tag="U2K71_000861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HEM7314967.1"
                     /translation="MWEKVEKKQGPIYRQIMEQIMREIENGRINPGSQLESERKLGQL
                     FGVNRSTVVHALEELRELGVLTSKRGSGWFVNQTEWGKFAVPRIDWRQMISPRYEQTD
                     WYEQKIREAKQIQKTSFLDLYASEMPDELLPNFEFPSYSLEEILAEEKEMDLLGYTPL
                     RNKIKRYLESKFELAFSPNQLLVTGGGQQAIFLILQTILSAGEGIAVESPSFFYRLPL
                     FKASGTRLFGIPMDEEGIDLEKLKESIHKNKLKAVLINPNFQNPTGKVMSQKRREKLV
                     ELCRQYRLPIIEDDVFTDLSFLNQEEVLPIRTIDPDNVLYVGSLSRVLGKTTKIGWVI
                     GPETFISQLAQAQEMMEFSMNILTQITVANVFNEAIDLKMQDVRKKLKEKSRLISAWG
                     DSQSFFKLHSILGGYYAWITWDGKKLDPELAEKVVDMGLGIAPSLLFGQDINGIRINY
                     SRLDENQIPLFYSKMKILEKWLTE"
     gene            49389..50498
                     /gene="pdhA"
                     /locus_tag="U2K71_000862"
     CDS             49389..50498
                     /gene="pdhA"
                     /locus_tag="U2K71_000862"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate dehydrogenase (acetyl-transferring) E1
                     component subunit alpha"
                     /protein_id="HEM7314968.1"
                     /translation="MANKKAPIDFQALLDEVNADFPVYQALDQDGNVVNKDLVPDLSD
                     DELVELMTHMVWSRVLDQRSTALNRQGRLGFFAPTAGQEASQLASAYAFDKEDVLLPG
                     YRDVPQLVKHGLPLSQAFLWSRGHAAGNSYPEELKALPPQIIIGAQYVQAAGVALGLK
                     KRNKKNVVFTYTGDGGSSQGDFYEAINFAGAYHANAVFYIQNNGYAISTPREKQTAAK
                     TLAQKAVAAGIPGIQVDGMDPLAVYTVSKMAREWAISGNGPVLIETLTYRYGPHTLSG
                     DDPTRYRTKDTDDDWQKKDPLIRFRKYLTEKGLWSEEKEEQVIEATKEEIKAAIAEAD
                     KVPKQKVSDFLKNMFEVSPQSIKEQIAIYEAKESK"
     gene            50501..51478
                     /locus_tag="U2K71_000863"
     CDS             50501..51478
                     /locus_tag="U2K71_000863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HEM7314969.1"
                     /translation="MAQKTMIQAITDALALELENDENVVVFGEDVGKNGGVFRATEGL
                     QEKFGEDRVFDTPLAESGIAGLSFGLALEGFRPVPEIQFFGFIFEAMDEVVAQMARTR
                     YRMSGTRNLPITIRSPFGGGVHTPELHSDNLEGLIAQSPGIRVVIPSNPYDAKGLLIS
                     SIRSNDPVVFLEHMKLYRSFREEVPDEAYEVPLDKAAVTREGTDVSIITYGAMVREAI
                     KAADNLAKENISVEIIDLRTVAPLDVETIIQSVEKTGRVVVVQEAQRQAGVAAQVVSE
                     ISERAILSLEAPIGRVSAPDTVFPFGQAENVWLPNAKDIEDKVKEIAEF"
BASE COUNT        16261 a         9000 c        11275 g        14994 t
ORIGIN      
        1 aaaaatcact ttacataagt ttcaaaagtt tctttaattt cctattgttc aataagtaac
       61 aatgtaaaga taaaattaaa aatgttaata cgtattagca aaataaaagc tttttttatt
      121 gtgaataaat gaacataatt aggaggagaa agatgaactt atttgacatt tttcgatcga
      181 ctttgacaga tatgtcgatt gtcagtgcaa tcacttcaac agtttttatt atattattag
      241 ggtttgtttg tagaaaaaag gggatttttt ctgaatctgt tggaaaaatt ctatcgaaag
      301 tcgtcttaac agtggctttg ccagcactgg catttcgagc tttcatgcaa gatatcaata
      361 cagaattact gatgcaagga atcaatgtct tgatttgggg attagttatt tatattattc
      421 ttattctagt ttcaaaacct tttttcttaa gctataaagg ggataagcaa acaacgttgc
      481 gtgtcctgac gatctttggt tctacgacct ttttcggaac gcctatcgtc agtgcgattt
      541 atggtcctgt cggcgtgatg tattcctcaa ttttcaatat tggctaccga atctttttgt
      601 attcttatgg atacataaaa atgagtggct tgaagatgga agctaaaaat atcaaagcga
      661 tgttcgtcaa tccaatcatt atcgctactt ttgccggcct tttcatttgg ctgttccaag
      721 ggtatctgcc acaagtgtca gttacaggtg cagaaggaga gataagccaa gtagcgtttt
      781 tgcgaattga ccagactgca ccgtggctat ttcagccgat ggattattta gctggtttaa
      841 gttcaccatt ggcgtggcta gcaatcggcg caacattagg tgaagtaagc ttcaaaaaag
      901 ctgcatctga aaaaacttca tggtattaca gtctagtcaa agtggtattt gtacctgcat
      961 tgaatatcgt gattctagca gttttgacgg caacgaatat tctaccagtc aattttgaag
     1021 cactggcaac aatcgttatc atgatggcta caccagctgc aacagtggca gcagcctatg
     1081 ctatcagttt tgatagggag gcacttttag cctccaatgc atccttactg tcaactattt
     1141 ttgctgtggt actgacacct gtatggatcg ttgtattaca aatgattcat ggcataggtt
     1201 tattttcata agaaatggag gaagaaagta tgagaatcgc atgttacggt gttcgtccga
     1261 acgagataga tttttttgaa aaattgaata tttaccatta tgatctcagt ctttatgaag
     1321 aactgttgac acatgacaat atcgtgtcag caaaggacca tgatgctgta ttgttgaggg
     1381 gaaattgtat agcagatgag accaatcttg caaaaatgca ggaatatggg attcgttatg
     1441 tttttacacg gactgtggga gtcaatcata tcgatctaca agcggcagct gattttggta
     1501 tgacagtagc ccgtgtacca tcgtattcgc caaatgcgat agcagagtta tctttgactt
     1561 ttgccatgat gcttttgaga aatactgcgt atacgacgat ccgaacatct ttcaaagatt
     1621 ttcgagtgga tgagcaaatg tttagtcgtg aaatcagaaa ttgcacagta gggatcatag
     1681 gaacaggcag gatagggttg acggaagctg ttttgtttaa gggactagga gccaaagtga
     1741 tcggctacta tattcatcag acagaagctg ctaaaaaagt attgacgttc cactcgcttg
     1801 aagagttatt agccaaaagc gatattgtca gtattcatgt acctcatatc ccaggtgaaa
     1861 acgatcaaat gatcaatgaa ggatttttag ctcaaatgaa aaaaggtagc atcttgatta
     1921 atacagcccg tggagagctt caagataatg aagctatttt aagagctttg aaaagcaatc
     1981 atttagctgg ttttgcaacc gatgtttttg caaatgaaac agaggttttt ttccgatcct
     2041 tcaagccatg ggagacgatt ccagaccctg ctatccagca attagtagaa atgtacccta
     2101 gagttcttgt aaccccgcat gtaggttcaa atacagatga ggcattatca aatatgattt
     2161 cgacaagttt cgagaacttt cgtgaaataa tagaaactgg aaaaacgaaa aatgaggtat
     2221 ctcttcctaa agcccgtcaa ttaagataag aaaacatgga tattcgtcaa taaaatgttt
     2281 aaaaatagtc caagtaaaca gttgttactt ctgtatactt ggactatttt gtatgggata
     2341 attcgatgac tcaacagtaa tctgtgagct ttttccgccc attctttgtc aattcataac
     2401 aggttagcgg ccggccgatt tccaaatatt tgattctttc gttcaatata tcttgttcta
     2461 ctaaaaaatt caaatatttc ttgacggata tacgtgacag tgagcttttt tgagccagtt
     2521 cttttgttga aaaaatagta tcttctttta gaataaatgc acttattttt tttaaagtca
     2581 gttttgacag acctttaggc aaatcgatga atgatggtat ttcgtgaatg ggagtggtgt
     2641 ggttgaatac ttgatcgatc aatttctgat cggctttttc agtcgtttgg attgtctgag
     2701 agaaagcaag atacttttcg atcgccagct gaaaacgttg caaagtgaac ggtttgatca
     2761 aataatctaa ggcaccgtag cggatcacat tctttatggt ttcgatatca tctgcagcag
     2821 tgatcaaaat cactgggata tcatttcctt gctgtctaag ccaagataaa aaatggatac
     2881 cactttcgcc aggcatgtat acatcaagca gcagcagatc tgcgtttttt tgtctgaaat
     2941 atttttttcc ttcttctaca tttctgaaac tttcgattac gttcatatta gggatgcttt
     3001 ccacaaaagt acgattcaat tctgcaacca ttggatcatc ttcgattatc aggacatttg
     3061 tcatcatttt gatcttcctt ttttggtaat tctagataga cgattgtacc gccatttgtt
     3121 tgtttggaaa attctaaaaa tccaccgtgg cgtttgacga ttgaatggat gagattcaat
     3181 ccaaacccac gattgattcc cttcgtcgaa aaacctagcg taaatatttt ttctgagatt
     3241 tcttctgata tcccgacacc gtcatcgcag acggtcacga tgaatacttc agcttctgtg
     3301 tcatagttta aatgaatccg gacctgtccg tttctttcat gtttttgtag cacggcttct
     3361 tttgcattat caagtaagtt ccctaacact tgcaaaagtg aatggatgga gtcatccatc
     3421 tccattttag ggataaaaga atcgctttct aacagcattt caacagattg ttctttggct
     3481 tcgtttattt tgcctaataa aaaaccggca attgcaggta ctttgatcag acttgagact
     3541 tggccaatct cttttttata attttgattt agctgctgga taaaatgggt gacttcttca
     3601 tacttcttca aatcaatcaa accagaaatg acatgaagtt tattcatgaa ttcatgggtt
     3661 tgagctctca tggagtttat atattgtttc gtccccccaa gctcctccat taatttcatt
     3721 acttctgatc gatttcgtaa agttgcgact gctcctaaaa aacggtcatt gctaaaaaga
     3781 ggtgtaacgc tagaaatagt ttctatccca tttaaataca tggattgagt ccgaacggtc
     3841 gtttgatgct caatcgtctc tttaaacaaa acgtcatata gattatcact gatttttttc
     3901 ctacttcgaa atccaatagc tgctctgttt ggtagaaaat gttttttgca ggtgtattga
     3961 tcaagaggat agttttatca gaagagatgg ctaagattcc ttcatctact tcattttcga
     4021 taatcgtttt ttcacttaac tgttctgcta tttcttgtgg ttcaagtcca agcagtactt
     4081 ttttcatttt ttgggcaaga aaaacggcgc ctatgatgcc tacgctaagt ccaataatca
     4141 atgaaaaaac aatcattttt tgtccatctt tgacttgatt gtcaatcgtg ttgtttgtta
     4201 agccaacaca aactgcacca ataatctctt gatcataata aatagggacg aaatagcgga
     4261 agccgaaccc taacattcct tctttttcag agaaaaattc ttgtcctcca atcacttttt
     4321 tcacatcttc atgacttgag aaaggaagac cgatgtaact tgggtctgcg tgagaatatc
     4381 gaatgccttc agggtccatg acaacgataa aatctaaatt cgatgcaatc atgatttcat
     4441 ttgttattcc ttgaatcgca tgtgtcgggt caccagtctg cagctttttt tggatgagtt
     4501 tatcattggc aacgatttta gcaatgctag agattttatc tgacacacta gatcggttca
     4561 tcgaaagaat aaaattcctg atcaatatgg cagaaacaat caaagaaacg aatgtggaga
     4621 aacaaacgac aaggatgatc gtgttctgga gctttaatgg ttttttctta aaaaaaggaa
     4681 acataggcta cctcgaaaga atttattgtg aaaattttaa catgaaaaca aaaatttaac
     4741 aaattgtcaa cgataaacac aaccttccta taaaaaacac cactgagtcg gtaaaagaag
     4801 ctatacagaa ttttcaaaca ttggtatgat agacgtagaa atggaaggag gcgtgtatat
     4861 gataggggga atcgcacttg agacgattta ttggtataca ttgctgattt gtgcaggatt
     4921 ggcagtccta ttgattattt ttggagatgt attcgatttt gatggcccgc ttgatccgat
     4981 gcttattatc ccttggttaa ctttcacaag cttatttggt tttctaggtg agcgtttaac
     5041 agatcaatca tctttatttt tatttattgc tagtggaggc atatcgatag tgttggtatt
     5101 tttgcttaat ttctatatat tgatgccgat gaaacatgcc gaatcaacga tgtcgtcttc
     5161 tgaaaaaact ctggaaggac aagtagctgt tgtgatcaca ccgatccctg tacgaggtat
     5221 gggagaaatt cagttgaaaa gtgttaccgg ttccattagt cgtccagctt gtttttacag
     5281 cccccaagaa cagacagctg agcggggaac gaaagtattg atcattgaga tcaaggaaag
     5341 aatctgttat gtcatgcctt atgaaagcat gataaaagct taaaggagcg tgtgttaaat
     5401 ggaaatacca gttgttttta tcgcgatcgc aatcgcagct ttgattttac tgatgttgct
     5461 gatcgtcttt gtagcgaagt accagacagc aaaaccggat gaagcgttga tcatcagtgg
     5521 aagttattta ggaagcaaaa atgtgcacat tgatgaaagc aataacaaaa tcaaaattgt
     5581 gcgtggaggt ggcgcattcg tcttgccagt ttttcaacgt tcgaatcgga tcagtctatt
     5641 atcgagtaaa ttggacgtct ccacccctga agtgtatacg gaacaaggcg taccggtgat
     5701 gtgtgatggt acttcgatca tcaaaatcgg ttcatctgta gaagagattg ctacagcagc
     5761 agaacagttt ttaggcaaga caagagaaga actggaaaat gaagcaagag aagtattaga
     5821 aggacacttg cgctcgattc ttggttctat gactgtagaa gaaatctatc aaaatcgcga
     5881 taagttcagt caaagcgtac aagaagtcgc ttctgtagac cttgcaaaaa tggggttgat
     5941 catcgtttct ttcactatca aagaggtcag agacaaaaac ggttatcttg actctttagg
     6001 gaaaccaaga atcgcccaag tcaaaagaga tgcagatata gcagaagcgg aagctctaaa
     6061 agaaacgcgt atcaaaaaag ctcaagcaga aaaagaatca caagcagctg aattacagcg
     6121 acaaacagag atcgcagaat cattaaaaga aaaagaactg aaattagcaa cctacaaaca
     6181 agaacaagat gttgctaaag caaaagcaga ccaagcctac aatttagaaa gtgcccgagc
     6241 acaacaacaa gtaatcgaac aggaaatgca gattaaaatc gtagaacgtc aaaaacaaat
     6301 cgaactggaa gaaaaagaaa tcacacgtcg tgaaaagcaa tatgattcgg aagtgaagaa
     6361 aaaagcagat gccgatcggt acgctaaaga acaagaagca caagctcaaa aagtcaaaga
     6421 agtgactgaa gccgaagcag aacgattccg tgtggaagca ctggcagaag cggaagccaa
     6481 taagacacgt ttagctggac aagcagaagc cgaagcagca ctagcaaaag gaaaagcaga
     6541 agccgaagca aaacaaaaga tcgccaatgc cttcaaggaa tatggcgaag cagctgtctt
     6601 gagtatggtt atcgatatgc taccgcaatt gatgcgcgaa gcagcacagc cgctaggaaa
     6661 tatcgaaaaa atctctgttg tcgatacagg aagcagttca ggagaaacca gtggagcgaa
     6721 ccgagtgaca aattatgcca caaatttgct ttcttctact caagaaacac taaaagcgac
     6781 aacaggactt gacttgaagg aaatcgtaga gaatttgtcg acacgtggga ctactagcca
     6841 agtaaatatg tatgaagcgc caaaagaggc agaacaaaac tcgatgaagg aagaataaag
     6901 tagtacactt caccgttcaa tcaaaaatgg aggaagactt tttaaagttc ttctctccat
     6961 ttttttattt tattgcaaaa gaaaattagt cctcgctatt tttcttttgt ttttggatct
     7021 tagcttctat ctcttgtaaa tttgttctta gcaatcgttc tttcttttgg agttcgacca
     7081 aatcttctga tagttcttcc ttcttttctg tttcaggagg acgaagaaag aaattgatac
     7141 cgattgcaat gaatgtccct ataaaagtat ctagtacacg ttggatagca tacaaagcgg
     7201 attcgccttg agggatgctc aacgcaatca gcaacaacgt agctattgcg gaaatgatac
     7261 cggaattatt gttaatacca tctgacacga cgatcacaat aataactaga ataggaagca
     7321 agatcaattc taccaaaaaa tcattgtgaa aatagctttt gacaaaaaaa taaatgatag
     7381 ctaatgtacc acctaatgta ttgccgatga tcctagactt tccaaaagaa acggttgtag
     7441 tcaagtcctg acgcaaagaa aaaacagcag ataatgcagc aatcatcggg attcctcgtt
     7501 gagtcaaatg aaaaaacaag atacataaaa aaacagctaa ggcacttttg atcgtacgca
     7561 tacctagtcg aaaacggcca aattgcatag agattctcct ttttggtctt tttactatca
     7621 tacctaaact tttgtctgat agctagtttt tcattattca agtataatta aaataaaaaa
     7681 gggggaagta aatgcgtata ggaatgcgaa cagtcaaaac agtgatcagt gtcttctttt
     7741 cgatggtcac agcgggtagc ttatcgctgt tgtattggcc agctgcaggg atcattgcat
     7801 tattaagtgt aggaaatacc aagcgctcta cattgcgtac gggcatgaat cgaatcgcag
     7861 cctttatatt agctacggca acagcactgt tcagtttttt tgctgttggg tatactattt
     7921 ttggcttcag tcttttttta ttcctgttca tcccgcttgc ctctcgcttc aaattaaccg
     7981 aagggatagt agtaaattcg gttttggtca ctcattattt agtggaggaa aacatgtctt
     8041 ggcagctgat cggcaatgaa gcgggattaa tggcgattgg attagtattt gctttgcttg
     8101 caaatgttta catgccagat cgtacgaagc agctgaagga aaaccaaata cagatcgaaa
     8161 aagaatttcg aaatattttg agacaaatgg cagaatttct tttatcagaa aataaagaag
     8221 atgtgcagat caaatgcgaa catttactga cattcattcg cgaaagccaa gaagatgcca
     8281 gagaacatca agaaaactac tggctcagac agccgctata ctacgaaact tacttctcta
     8341 tgagaagagc acaggccaat gtgattaaag acatgctgga aaatctagag agaatccagc
     8401 aaccagctta ttatggaaaa catatctatg gtttattgat ttatactgca gaaacatttt
     8461 cagagtcaaa tgatggcaga caaattttaa cacgtattga agaagtttat gtattgtatc
     8521 ggcaaatgcc tctgccgact agtcgccctg aattcgagga tcgagcagaa ttgttccagt
     8581 ttttgcagtc ttttaaaagt tttattgaaa tcaaggcaga attctcccaa caaaaaatcc
     8641 aaaaatagag gttgtgaatt cagctaatat tcaggtcaaa aagctgattt cacaacctct
     8701 tctttaaacg gtgttctcct gtcaactcct cggtcacaag tcacaatatt caacaaacat
     8761 gagaagctgt tttttgaaat tgctccttgt gactcggagc tgaacggtag gctcacaacc
     8821 tctttcttaa acggtgttca aaagctttcc tctgcggtaa taagtccagc caaacgaaaa
     8881 ataggaggag ctatttttgt ttggctgttc ttattacttg atgcagaaca gctttttcac
     8941 aaccttttaa aattacatat ttttccatag aacttgcatt tgatccaaga tttcgatgac
     9001 atcttttgtc aagaagagag agcgattggg caagtcacct tcaatcatgt gtaccatatt
     9061 ctttatttcg taattcattg cttcagatgt attcccgctt tcgatgaatt cctttgtacc
     9121 atctgtaaat aagatctccg cacgatcagc acgcgggtaa tcagcaatcg tcacataacc
     9181 gttctcaaag gcaacgatac cgacttttgg catttttgcc tgaaaagtca agctgacagc
     9241 agcaagttca ttttctttgt tacgcaagat agtgacggat tgttcgtcca cgcctgtttc
     9301 aaaaggaacc attgtagagg caactacttc aggttgagag ctcaaaaaga aacgagcgaa
     9361 agatacagca tacgtaccga tatccaacag agctcctcca gctaattctg gattgaagaa
     9421 ccggttttta gggtctggtt ctttgtaact gccgaaaggt gcttggatca tttttaaagc
     9481 ccccagtttt cctgtatcca ttattgagcg taactgctga taaagtggca tgttgaagat
     9541 tgtcatcgct tctgctaaga tcaaattctt ttcttctgct aagcgcatag cgtcagccag
     9601 ttctttttta ttcatcgtga tggctttttc acatagaacg tgtttgcctg cttccaatgc
     9661 tgccaagata tggtcgatat gctgtctatt tggtacagca atgtacacga tgtcgatttc
     9721 agcatcgtct aacatttctt gatagcttcc gtaagctttt ggaatattgt aacggtgagc
     9781 gaagttttcc gctttttctg aagttctgga agcaactgct gttagttcgc tcgtctcttg
     9841 attgaagctt tcggcaaact catgtgcgat ggtaccaaga ccaacgattc cccattgata
     9901 ttttttcatc tgaaaacgct ccttcatagt tggattgagt ttcttgtctg tctattatta
     9961 taacaaaaat cacgaagaat aaagaggaga ggacttgaaa cattaaaaaa aatgaaagca
    10021 gcgatttaag aggtgctttt tgatcaaaac ttaagtagaa tagttaaaag gaggggataa
    10081 acgtgcatca agtatatatt gatcttatta tgagtgtatt attagaacag tttgaaactg
    10141 aaaaaaattt tttagatgaa tacttgctga tcagtccaga acaatggcaa aaatggaaag
    10201 atggcgagaa aaatctgcca tctgaagcga tgcagaaagt aaaaaatctt ttcactgatt
    10261 atgaatggat gctaacgcaa aagattttgc gtcaaacgat tatttttccc gaaaaacgaa
    10321 acgttgccgt tgcagagtac aagcaaatca aaacacgcat cgcgcaaaaa tggcttagca
    10381 gcaaagcagc aaaagtcgag ctgatccctt tgaaaaataa tcagcaggct ggtagatacc
    10441 ttgacttgaa agtaagtatt caatatgatg aatggggcta tgatgatatt ttgaattttc
    10501 gtctgccagc agatgttcaa aaacagatcg aaggagaaaa aatcgaactt ctcgattggg
    10561 tgaatgaaaa tctggaagaa acttacgttc atgaggcgaa tggggaacgt gaagaacaac
    10621 gaaaggattt ttcaagtgaa taaattttta ggatttggag cagttgtgat tatgatcgca
    10681 gcaatgggct atgttatttt tagcgatgat gattcgcatg ttcaagccgg caatcaagca
    10741 gtcgaacgaa caagcaccaa aacaactgct agtaaagaaa agaacagtca aacaaccagc
    10801 agcaaggtaa aggacaaaga aacaaaagat cgtacgtcca aacaattgcc agatgttaaa
    10861 acagacgatt ggaagctagt tttggttggt ccagatcaca aattagaaaa agaaattgat
    10921 gagtctactc aattggcatc attaagtaac ggatacttga tcgacaaacg cattgctcca
    10981 gaatatgaag catttgccaa ggctgctgaa gcagctggat ttccattagt gatggtctca
    11041 gcttaccggt ctgtatcatc ccaacaacaa gttttcgaac aaaatgtcca agatgtgatg
    11101 agtcgtcaag gtgtatcaga agaggaggcg acaaagatta ctaaagaaac gattacggag
    11161 ccaggataca gcgaacacca cacaggtctg gcagtcgatg tcgtcgatca aaactggtac
    11221 aacagttatc cttctactgt attggatgca agttacggag atcagcctgg agctaaatgg
    11281 atcgcagata atgctgcgca gtatggattt atcgttcgtt atccaaaagg acacgaagat
    11341 ataactaaga tcacatatga accttggcac ttacgttatg tcggtaaaga aaacgctgag
    11401 tatatcacca aacaccaatt gacgctagaa gaatatctga atgaattaaa tgaaaagtag
    11461 gaattgatca tggcgaaaaa aaagtggaaa aagcaaaagc gcaagaatat ccgttggcca
    11521 gcagtggttg ccggtgtagc agtgatactc ttagcctttg ttttttcctt gaagaatctg
    11581 tcttctcctt ttactgataa tcagcaagaa aatgaagcgc aatcccatca gcaatttatt
    11641 gaacggcttg ttccgcatgc ccaagaacta caagatggtt atggagtgct tcctagtatc
    11701 attttaggac aagctatatt agaatcaaat tggggaaaaa gtaccttggc tagcaaatac
    11761 aataatcttt tcgggatcaa ggcttatggg gatcagaaga aagtaagcct ggaaacaaag
    11821 gaatttgtta acgaagaatg gataactatc caaggagact tcaaagtgta tgattcatgg
    11881 gaacagtcca tggatgacca tacacaactg ttcgtccaag gagtagattg gaatcctgcg
    11941 ctgtatgaaa aagtgattac ggccaccaac tatcaggaag ccgcacaagc attgcaagat
    12001 gcaggttatg cgacagaccc cggttatgcg caaaaaatca tccaggtcat cgagacgtat
    12061 caattgaatc agtatgaccg gagatagcaa aggagaagtg gctgatggca gaacaattta
    12121 cgccagaaat catgacggaa ttgagaaaag atatcgtaag agtaccggaa atcattttag
    12181 aagcaagcgg gatcaagata tttggaaaat tgatccgttc gatcatcttt actacggata
    12241 ttgcgatcat acgtaataca aatgcgaatg cagtaattgc tgtgtatcct tttacacctc
    12301 atccagcgat tactaaaagc attattgagg cagctgacat tccagttttc tccggtgtag
    12361 gtggtggttt gacacaaggt gctcgttctt cctacatgag catgttcgca gaagcgcaag
    12421 gttcgttagg ggtcgttttg aacggtccta cgcctttaga gacagtaaaa gaagtttgtc
    12481 gagtagttga tattccagtt atcagtacgg taacgtcgaa atatacagaa atagatgaaa
    12541 agttagcagc tggtatttcg attatcaata tcagtgcagg taaagaaact gcagaagcag
    12601 tacgccattt tcgtaaaaaa tatcctgaac ttcctatcat tgcgacaggt ggtccaacgg
    12661 aagaaagtat tgctgagacc attcaagcag gagcaaatgc catcacatat acgccaccaa
    12721 gtagcggaga gctttttagt cgtaaaatgg aaaaatatcg ggaaactgag aaaaagaatc
    12781 atgaataaca ggtgagaatc aaaagacgag gaagagcaga tagtggctct tcctcgtctt
    12841 ttgtactcca gtactatttg ttcagaattc aaaaaaaaga gaaatttttt tgttttttta
    12901 cctgtaatgt acataagaag agttaaaacc ttttgttatt attagcattc taacaaaaaa
    12961 atgtcttgat ttattgaagc gctttctata gaatgaaaaa cgaataaaaa ctaaaaaagg
    13021 cacccgacaa tttagtagca gctaaattgt cggggcttga aaagtcagcg ggaactgacc
    13081 aatcaagttg ccatagtcaa tgcttgtgca tcgacgtgta tattgtacct aatttcatac
    13141 agaattacta gttatttttc tgttttttag ggaagagcgc acacgaagaa gcatgcttat
    13201 ttagctatgt caaggatatt gggcagttgt ctgatatcct tttttctttg cgtaattatc
    13261 ctcccatcat tgaagcaaga agtgttttta gcgatgaaag aagaatcagc actatctaat
    13321 ttttcttgaa agtattgagt tgttcatcct aggcagaacg ataaggcaag tcttggtttt
    13381 tattcccctt gaaaaagatc gtttaataaa tatgttctgt ataaaaatac agtatattga
    13441 aaaataacaa tggaagtaga ggtgcagaga atgaagaaaa gaatgagaat catcttagct
    13501 ttaagtgtta tgttattctt cttgacggct tgtgggcaag aagatgacaa aaaaatcacg
    13561 ctgaacatgg cttggtgggg ttcgcaagtc cgacacgatg caacagtaaa agtaatcgag
    13621 ctttatgaag aaaaaaatcc ccatgtgacg atcgattatg aattttatga ttatgaagga
    13681 tattacacaa aattaggtac actagcagca tcagatgata tctgggacat tttccaactg
    13741 aacaatcaat ttcctcaata catcaatcag attgaaccat tagatgaata tgtggagcaa
    13801 gggataattg atatttctaa tgcagatgaa tcctatttag caacgacgac ttacgacggg
    13861 caattagttg gactgtctaa cggggtgaat tcgtttgcga ttgcttacaa taaaaatata
    13921 tttgaacaat taaatatccc tgaaccagct tcaaattgga catgggatga atttgaacaa
    13981 atcagctatc aaatcactga agaattagga atctatgcta tttcaagatt cgaagatttt
    14041 atagctggct gtattattca aataccacaa gttgagaaag gattgaattt ttataatcgg
    14101 gaagatctat ctaacagctt gggatttgaa aatccagact atctaacaga ttttttccag
    14161 ctgcgtaaaa atctggtaga caaaggggcc catccagaac caggtggtgg agctgcaagt
    14221 acgacggatc tgtcttttca ggcggttagg gatagagaag cggcaatggt gtttctatca
    14281 agtaaccagt tgacagaaat attgtcaggg gctcctgaag atatggagat tcagttgatt
    14341 aatccacctc gcagaaaagc agatggagaa tctggtatcc ctttacgttc ttcgcaaatg
    14401 ctgagtatgg caaaaagctc agcccataaa gaagaagctg ctaagtttat tgatttcttt
    14461 caaaacagtg aagaagccaa tgaaatttta aaaggagaac gtggtgtacc aatcatgtcg
    14521 aatattcgag aacagctcgc tcgtgcagat gatgcaactt tgcaagcgac atttgattat
    14581 ttagaaatga tttccgatat ggacaacggc gaagtgaatg tctttgatag tgctttgaat
    14641 ccggaaattc aagaccaata caatctttta gttgaaaaag tcatttatga agaagtaaca
    14701 gcagaagaag cagcagagag tctttatgag tttgcgtcaa acattctagg atagcagcag
    14761 gaggaaggca tgaaaaaaga aaaactaaaa cggttcttgg atcaagacaa tgtggtcggt
    14821 tacgtatttg cttctccatt catcctagga tttctattga taacagtgat accgatgatc
    14881 ttgtcacttt attattcgtt caccgattat cgtttgggaa atccagtctc atggatcggc
    14941 ttggataatt atataaagat gctgagtgat gcccgttttt tgaactccat ccgagtcact
    15001 tttctctatg tatttgcatc tgtacctgtg aaactgatct ttggtctatt gattgcttat
    15061 cttttaacga ggaatatcaa aggaacgact ttctttcgtt ctttgtatta tgtgcctagt
    15121 ttgatcggtg gcagtatttc tatcgctcta gtttggcggg agttgttttc taaaaacggg
    15181 ttagtgaata ttttcctgga acgattaggc gcacctgcgc tttcctggtt tagcgatcaa
    15241 aaactggtca tgatccctct tgttttgatg tctgcttggc aattcggttc ggcaatgatc
    15301 atctttgctg caggtatcaa agagatccca ggctcgtatt atgaagcggc agagatcgat
    15361 ggagcgaaca aatggcagac gttttacaag atcacgttac ctcttctttc gccgatcatt
    15421 ttgtataact tggttatgca gacaatcgcg gcgtttatga cttttacgca ggcatttatc
    15481 attacccaag gtggtccgaa tgatgccacg aacttttatg ctctttatgt gtataaccaa
    15541 gctttttctt ttaattctat ggggtatgct agtgctattt cgtggatcat gctgttgatt
    15601 atgggagctg tcacctttct gattttcaaa tcatcaaaaa aatgggtcgt tgtgtgaatg
    15661 agaggaggaa gaaatgaata atacagtaac gaaatggttt caatacggtt ttattttgct
    15721 attaggtcta gtcatgattt atccggtctt atggatgatc gcaggttctt ttaaaacgga
    15781 tcaagagatt atgagtggtt cgctgaattt gattccagag acttttcaat gggaaaatta
    15841 cgcaaaagga tgggcggggt ttgctggaat ttcatttttt actttcttta ggaattcgtt
    15901 tgtcatctct gcagtttcga cgatagggac agttctgagc tcgacttgta ttgcttacgc
    15961 attatcgaga atcgatttca aaggaaaaag attctggttc gtcgtcatgt tagtaacgat
    16021 gatgattcct gcacaagtga ttttgattcc gcagtttatt atttacaatc ggctgaattt
    16081 ggttgggacg tatgtgccgc tgattttacc acattttttt ggacaagcat ttttcattta
    16141 ccagatcatg caattcatgg tcagcatccc caaagagtta gatgaatcag cgattattga
    16201 cggatgcagc aagtatagtg tctttaccaa aattattttt cctttgctga aaccttcgat
    16261 catcacgacg atcatcatcc agttttattg gaaatgggac gacttcatgg gaccattgat
    16321 ttatttaaac aagccgagat cttacacggt atcgattgcc atcaaattat ttgcagacgc
    16381 tggatctagt acgacttacg cgagtatgtt tgcgatgtct acgttgtcgc tgctaccggt
    16441 ttttctgatt ttcttattct tcaataaata tcttgtccag gggatcagta caagcggtct
    16501 gaagggctga atatgaaaaa gccaatagaa gagatgtttc tttacaagct caactactat
    16561 gtctgtcgga tttttttaac cagtcttatc agtaatgctc ttttcgcttt agttctagga
    16621 tgtttgttca ctttaggatt agtgctgtct gctcgtgttg tctatctgat ttattttttg
    16681 atgatgtatc cttcttatta tctatctgtc cattacttgg cagaaaaaaa ggtatgttgg
    16741 aggaaaggaa aaatcaaacg gatgtgtttc atcattacgt tgtttcagct gtttgtcatt
    16801 atttttgcag ttgattggta tttattagtg ggaaagattg gagcttggta tctgattcct
    16861 tttctattgt gtctgttgct ttttgtatcc catagttttt ttagtacgct aattgatatc
    16921 tgcaaagaag ataaagcaga tagtcttgtt tatttgatca aaaggaatat ctggctgttg
    16981 tcaaaaaacg tcaaggggtt tctgtggtat ttatgccttt tgtatggact gttctggtgt
    17041 atacagcatg attttttact cactgcatta ctgtatccag ggctgccatt tgtcacagta
    17101 ttttgcttaa aagaaaagga gaatgacttt aaatgaagaa aaaatatgta caagtcggac
    17161 ttggtgggag agcgcgtttt ttttatgagg caattgcctc tacgtatcat caaacttgtg
    17221 aactagtcgg tttttgtgat gtatctcaaa caaggatgga ttatgcaaat cggatacttg
    17281 tgaacaagta tggacagaaa aaagtaaaga catgtatgcc atcacaattt gagcaactac
    17341 tggatgacgt acggccggct gaagtcatta ttacgtcagt agatcgaacg catcatgatt
    17401 atatcattcg tgcaatgaaa aaaggatatg atgttatttg cgaaaaacct ctgactatcg
    17461 atgaggaaaa agctcaagaa ataataacgg tccaaaaaca gacgggaaga agcctgcgag
    17521 tcacttttaa ttatcgttat gcaccgtatc acacgaagat aaaggaactg atagaaagtg
    17581 atgtgattgg tgatgtgttt tctatccact tcgaatggct gttaaatacg caacacgggg
    17641 cagattattt cagaagatgg catcgaaata agcacaatag cggcgggctt cttgttcata
    17701 aatctaccca tcattttgat ctagtgaatt tttggttgaa cagccgtccc caaaaggtat
    17761 ttgcattcgg tggattaaac ttttatggga tgcataatgc ggagaaacgc ggagtagaaa
    17821 tattttacag tcgatgctat ggaagcgaac tagctaaagc tgatccattt gctatagacc
    17881 tgtcagccaa tgaggaattg cgagaaatgt atttagaagc agaaaaagac agtggatata
    17941 ttcgtgaccg cagtgtattt tccgatgata tctctattga agatacacta ggcgtaacgg
    18001 tcaaatatga aactggtgcg attttgacgt attcgctaaa cgcgtatatg ccgtgggaag
    18061 gattcaatgt ttctttcaat ggcagcaaag gacggatcga aatgaaagtg atcgaaaagt
    18121 cttacatcaa tgcaggtgga gataaaaagg atgaaggagc agccgttctt cagtccatcc
    18181 atgtctatcc gatgttcggt gagccatatg aggtaatcgt cgaacaagaa gcgggcggac
    18241 atggcggagg agatccgcgt ttattagatg atctatttgg tgaagaaaaa gaagaagatc
    18301 cgtggaatcg ggcagcaact cacgtagatg gtattttgtc gattttgaca ggaatcgcag
    18361 caaatcattc tattgcttca ggaaaagcag tagtgattga cgagcttgtc agcttaaaag
    18421 aataagaata gatttacgga aattacttcc cgtagtttat aaaatcttgc cttattgccg
    18481 ataggctgtc ttttgaacac tatttattcg gaaaaagggg ctatgacatg ctttttgtca
    18541 tgccttcttt ttatgtgggc tgctttttta gataggagaa aataaaaata actatttaaa
    18601 cccgaacaaa aaggaataat gcattaaaaa tcattagatt ttacaaaaaa ataagtaaac
    18661 cacgttgtca gtttcagaaa gtagtgttag actgttttca atatttgaaa caacattttt
    18721 tattcaaata tggcaaactg ccttcacgtg caaaacagtt gcaagaaaat gaatttacac
    18781 tattattagt tttacatacc tacttatata tcgtcataat atggatgaca gtttctagcc
    18841 agtaccgtaa atgctggact ataagtaaaa gattggctat ttcacccttc ttttcttatg
    18901 ataaagtgga gggtgttttt ctttaacaga aacatagtag gtttttgcac aaaagcggtt
    18961 atttcggtat taataaatga taaaggagca aataaagtga aagaattagt tgaacgtatt
    19021 caaaaagacg gacgtatatt aggcgagggc gtattaaaag tcgacagctt cgtcactcac
    19081 caagtagacc ctgtattgat ggaacaaatg ggaaaacggt ttgcagaagt atttaaagaa
    19141 caaggaatca cgaaagttgt gaccattgaa gcttctggga tcgcaccagc tttatttgct
    19201 gcaagagaat tagatgttcc aatgatcttc gctagaaaag caaaaagttt gacgatggat
    19261 gaagaattat tgacagcttc tgtttattct tttacgaaac aagtgacaag tacgatttct
    19321 atttctcgta aatttttatc ttcagaagac aaagtattga ttatcgacga tttcttggca
    19381 aacggacaag cggcaaaagg attgatcgaa ttatgccagc aagctggagc gcaagtagaa
    19441 ggaatcggga tcgtcatcga aaagtctttc caagatggac gtcagttact tgaagaaatg
    19501 ggattgagag tcgtttctct tgcgcgtatt gcttccttga cgaatggaca agttgagttt
    19561 cttgaggagg acgcataatc gtggaaaaag aaacacaaaa tggaaaagct gcggttttag
    19621 ggctgcagca tctgctagcg atgtatgcag gagcagtagc ggtgcctctt ttgatcggta
    19681 caggactagg ctttaatcaa gaacagatga cttatttgat ttcaattgat atctttatgt
    19741 gtggattagc gacattattg caattgacag ttaaccgatt tttcggaatt ggcttgcctg
    19801 tcgttttagg atgtgcgatc caagcagtag ctccactgat cttgatcgga accaatgaag
    19861 gagtaggagc tatctacgga tctattatcg cttctgggat ctttgttgtg ttagtttctg
    19921 gtgtcttttc taaaattaaa aaactattcc ctcctatagt aacaggaacg gttatcaccg
    19981 ttattggttt gactttgatt cctgtagcaa tcgagaaaat gggtggaggt aatgcaacag
    20041 cagctgattt tggtgacaaa acaaatctgc tacttgcatt tgtgacgatt ctcctaattg
    20101 taggcgtgca aatgctggca aaagggttca ttcgttcaat cgcggtattg attggtttag
    20161 tcggaggaag catcttagct gctttcatgg gaatgattga tctgagcgcg attggagatg
    20221 cgccgttgtt ccatgttcca caaccctttt attttggtaa accaactttc gacgtatggt
    20281 cgattttact aatgatcatc atttccatgg tcagcatggt agaatcaaca ggtgtatatt
    20341 ttgcacttgg tgacattact gggaaaaatg ttggagaaga agaattgaaa aagggatatc
    20401 gtgcagaagg acttgccgtt atcttaggcg gtattttcaa tacattccct tatactggct
    20461 tttctcaaaa tgtcggattg gttcaacttt cgggaatcaa aacaagaaaa cccatctatt
    20521 tctctgcatt cttcttgatt gttttaggat tattccctaa aatcggtgcg ttagcacaaa
    20581 tcatccctga gccggtactt ggtggaggaa tgttagtcat gttcgggatg gtggcagtcc
    20641 aaggaatccg tatgctgttg gaagttgatt tcagcaatga taaaaatcta ttgattgttg
    20701 ctgtttctgt tggatttggg ctgggtttca atgttacgcc gacattattc agtcaaatgc
    20761 cgcaaacatt gcaaatgttc actggaaatg gcatcgtaat gagtagcatt acggctatca
    20821 tattgaattt gatctttaat ggattgaaaa aagatgaaga aatattggaa gaagccttca
    20881 agaagtaata gagcaaaaga aaggttgtga aatcagcgtt ttgacctgat tgttagagaa
    20941 acaaacggaa aaatagttcc tcgtattttg ccgtttgttt gagctaatac cgagggtcag
    21001 ctgattgaac accgttgaag gaagaggtcg tgagcctgcc gttcatctcc gagtcacaag
    21061 gaacaatttc aaaaaaaagc ttctcatgtt tgttgaatat tgttccttgt gaccgaggag
    21121 ttggcaggag aacaccgttt atccataaaa aggaatgtga cctaactttt gtcacattcc
    21181 ttttttttat ctttaataaa cacgaacaat aaaatgtttt tctttgttaa cattcgaaat
    21241 ttctttgaca tcaaaaaaag tgattgttaa aatatgaatg tagaaaaact gggaggaaaa
    21301 acaatgacag caaaaatctc tgtgattatg gggagtactt cagattggga aacaatgaaa
    21361 gaagcatgtc aaatattgga tgaattcggt gtgtcttatg aaaaaaaggt cgtttctgct
    21421 catcgaaccc cagatttgat gtttgaatat gcacaaaacg ctcgaaaaaa aggaatcaaa
    21481 atcattattg caggagcagg gggagccgcg catctgccgg gtatggtcgc atcaaaaaca
    21541 actttgccgg ttatcggtgt accggtccaa tcacgaacat taaatggttt ggattcgctt
    21601 ctttcgattg tccaaatgcc tggaggagta cctgtagcta ctacagcaat cggaaaagcg
    21661 ggagcaatta atgctggact acttgcgata cagatgcttt ccatgtatga tttagaattg
    21721 gaggctaaat tggctgaaag acgttcaatg ttggctaaaa ctgttataga aagtagtgat
    21781 caacttggtt aaacctttgt tgcctggttc aacgatcggg atcgtcggtg gcggtcaatt
    21841 aggtcgtatg atggcgatta gtgcaaaaga aatgggcttt aaagtaggtg tgctggatcc
    21901 tgtgagcgat tgtccagccg cacaagttgc agattggcac attgaaagtg tctatgatga
    21961 cacctttgcg ctgaaagagc ttgccagaag aacagatgtc attacctatg aattcgaaaa
    22021 cgtcagtgta gaggcgttaa atgcgatttt acccatgtca tttattcctc aagggacaga
    22081 cttgcttgcg attacgcaag accgcctgtt agaaaaatct tttttggaaa cgaataacat
    22141 cgtgattgct ccttatgcaa cgatcgtcag tccgacggat atccaagatg cgattgacgg
    22201 gatcggctat ccttgtgttt tgaaaaccac tcgaggcggc tatgatggca aggggcagta
    22261 tgttttgaaa agtcgggcgg atctagctcc tgccatggac cttttaagag aaggaacctg
    22321 tgttttagaa gcatggatcc cgtttgaaaa agagatttcg atcatggtgg caggaaatgg
    22381 tcaaggagat ttcacgactt ttccggtcgt ggaaaacatc caccacaaca atattcttca
    22441 tgaaacgatt gctccagccg cgattgatca ggatgtgatc gaggaggctg agcgtattgc
    22501 ccgtgtgatt gcagaagcag tgagcctttc aggtgtttta ggggtagaaa tgtttttaac
    22561 aaaaacaggc gggctatatg tgaatgaatt agctcctcgt cctcacaatt ctggacatta
    22621 ttctattgaa gcttgttcta tgagtcagtt cgatgcccat attcgaggta tttgtggttg
    22681 gccattagga gaaaaagtac gtttgataag caatgcagta atggtcaatg ttttgggaga
    22741 acaactgctg gaaagctatc gcttgattga gaataagcca gactggcaat tccatttcta
    22801 tggaaaaaaa caagcaaaaa aaggtcgcaa gatgggacat atcactatct tgacagaaga
    22861 catccagcaa acgctggaag aaatcaaaca aacgaatatt tgggattaga ggagaaaaaa
    22921 catgttagaa cgttatacac gccctgaaat ggggaatatc tggacggaag agaacaaata
    22981 ccgtgcgtgg ctcgaagtcg agattttagc agatgaagct tgggcagaac ttggtgaaat
    23041 cccgcaagaa gatgtgaaaa aaattcgtga gcatgcatca tttgatatcg atcggatctt
    23101 ggaaatcgaa aaattaacac gccatgatgt cgttgcgttt acacgagcgg tttctgaaac
    23161 attgggagaa gaaagaaaat gggtccatta tggtctaaca agtacggatg tcgtagatac
    23221 tgcttatggc tatctcatca agcaagcaaa tgatatttta cgggaagact tgaaacgttt
    23281 tacggaaatc attggcgaaa aagcaaaaga acacaaatat acagtgatga tgggtcggac
    23341 acacggtgtc catgctgaac caactacttt tggattgaaa cttgccacat ggtattcaga
    23401 aatgaaacgg aacatcgaac gatttgaaca tgcggcaaaa ggagtagaag caggaaaaat
    23461 ctcaggagca gtgggaacgt tcgcaaatat cccgccattt attgaagagt atgtatgtaa
    23521 acaattgggg atccgtccac aagaaatctc tactcaagtt ctccctcggg atctacatgc
    23581 tgaatatttc tcagcgatgg cgcttattgc tacaagtatt gaacgttttg caacagagat
    23641 acgcggattg caaaaatccg aaacacgtga agtagaagaa ttttttgcga aaggtcaaaa
    23701 aggttcttct gctatgccgc acaaaaggaa tccgatcggt tcagaaaata tggctggtct
    23761 agccagagtg gtgcgcggac acatggtcac tgcctatgaa aatgtcacat tatggcatga
    23821 aagagatatt tctcattctt cagcagaacg aatcatcttg cctgatacca cgattttatt
    23881 gaattacatg ttaaatcgtt ttggcaatat cgttaaaaat ctaacggtct ttcctgaaaa
    23941 catgaagcgg aatatgtctg caacatttgg tttgatttat agccagcgcg ttatgctgaa
    24001 attgatcgat aagggaatga caagagaaga agcttatgat cttgttcagc caaaaactgc
    24061 gtacgcttgg gacaaccaag tgcagttccg cccgctgtta gaagaagatc caaccatcac
    24121 atcggtatta tctaaagaag aaatagacga tgcctttgat tatcatcatc atttgaaaaa
    24181 cgttgacgtg atattcaaac gagtaggttt ggaataaaga agagtgagtt gcaaaattca
    24241 attaaaatag aatagcaaaa ataatcgagt agaggggaaa gccaactgac cttccgcccc
    24301 tctcacacca ccgtacgtac ggacccgtat acggcggttc aataagttga gtgtacgaac
    24361 tggtacttca aggagatgtc ctcatatcct tggcgggcca gttctttgtc tgttaatgat
    24421 cgatgaagaa tatgactttt ggagactgac caaatacctt ttcttgtatt tgaccattca
    24481 tacgctttcg ctttatcgat ccctaaacga atcagatttt ttcttttggt tcttggattc
    24541 ttccattgtt tccaaatata ttgtctgatt cttcgcttca accaaccgtt taactccctc
    24601 atgaattctt tcatctcacc aattccatag tagttgatcc aaccagtcat tagttgcttg
    24661 atttctttta aaataagttg gatacttctt ccacggtttc ttttagtgag ttgtttgagc
    24721 ttgcttttca cacgctgttt tgcttttgcg tgagggcgaa ttttaatacc tttctttgtt
    24781 ggcagaatgc agaatccaag aaactttcgt ttcaatggac ttcctaccgc actcttttct
    24841 cgatttaccg tcaactttag ttgttcttcg agaaattgaa tggatttctt catcacacgc
    24901 tgtcctgctc gtttgcttct aacatagata ttgcagtcat cggcgtatcg aacaaattta
    24961 tgtcctagat tttctagcac ttgatcaaat tcgttcaggt agatgttact taacagggga
    25021 gagagattgc ctccttgggg cgttccttct tcagttgttg tgatgagtcc attttccatt
    25081 acaccactca ttaagtattg acggatcagt tttaggactt gtttgtcctt tactttccgc
    25141 tcaatgaaat acatcagctt atcgtgattt acggtgtcaa agtaggcttt catatcaatg
    25201 tccaccacat atcggtaacc ttgttcataa taagattttg ctttcttcac tgcgtcatgt
    25261 gcgctgcgat ttgggcgaaa cccataacta ttatctgaga attctggttc aaatagcggt
    25321 tgtaacactt gattgattgc ctgttgaagc atacgatcaa taaccgtggg aattcctagt
    25381 tttcttacac cgccgtttgg tttcggtatt tctactcgca aaacaggctg aggtttatag
    25441 ttcccttggc gtaaattgga tagtaattcc tcgcggtgct ctttcaagta aggaagcagt
    25501 tgatcgacgg tcatcccatc tgcaccagcc gcccctttat ttctgactac ttgaagatac
    25561 gcttgattga gattattccg atctagaatt ctcccaaaca gattttctac accatcttgt
    25621 ttcctctgtt caccgcaaga agtgctgcgc acttcatcat acccttcgtg ttccacacta
    25681 gctctttgat gatagccaag ttttcttgtt ttctgcggtc gtcgcatctt ctcacacctc
    25741 ctaaattatt caagattctt attgttcagt ccttcgtaac ggtcagtgtc gttactacta
    25801 tgacctcggc tgacttctca tgattcacca tcatgtcgcc atgatcgttg tgctggtttg
    25861 ttttcattca acgcccacaa caccctcatg agatctcccc cggtaagagt gaaaactttc
    25921 ctcccatgta actgctagat ttactgtata ggtttcgggc agtattggac tttgtcttgt
    25981 gatgaagact tatccgacct aattcagcct tgtatctagt ttctgttcgt cagttcagga
    26041 gtttgcgtcc gacttccttc agccacaacc tcacgattgc cgccttgtct ttcgctaaca
    26101 gttcctactg ccaagcctgt agtggacttt caccaccaag ttttcaccca tgcagggcgc
    26161 acaccaaaaa agaagcagat ccgttagcgg ttctgcttct tttagtttat tatattttct
    26221 tatcaataat aggaacctga ttttaaaagc ggctacctct ttaccgtgcc tctttacata
    26281 caaaaaatag aggaattaag cgtaaatggc ttggctatta taactaaaat gctggttatg
    26341 gagatgttgc agcaagtttt cgagaaaagc gtattccatc tcttgtccat tgaataagat
    26401 ctggatacga taatgatttt tgaaacgctt ctgttcatga atctgactga ttgaaagttc
    26461 gcctttgtgt tctgtctgat aaagaaaagt ccttttacgg cattcagcac agcgcttggt
    26521 tgctcggcgg atttttcgga atgtataaaa actagtatct ttattgcata aataaacgtc
    26581 tttgatcgac agcaaatcat tatatagaca ggaacgttct tcagctgtta attctgataa
    26641 aaaaagctga ccttgtttta ttgcatcttc catcggcaag tgcctcctcg aacccattat
    26701 ccaaattttt agttaattct aatataccat cattaagtat aaatgtaaac gcttgttgat
    26761 ggtataagat tttcgtttct gataaaacac gaacattaaa agaatctaat gccttaaatg
    26821 tacatatttt tgttgattta taaaaagatg attgctaaaa tttaaagtga aaaaacgaac
    26881 aaaaaacgat gtatttgctt ttaatgtaaa taaaaaggag agacgtacat ggagaagaaa
    26941 gaacttttgt atgaaggaaa agccaaaaaa atatatctta cttctgatga gaacttgatt
    27001 tggatagaat atttagatca agcaactgcg ttgaatggcg cgaggaaaga ccagataagt
    27061 ggaaaaggag aattgaataa tcaaatcaca agtgcaattt tccgttatct gactcaaaga
    27121 ggaataaaaa atcattttat tgaacaattg tctaaaaccg aacaattagc aaaaaaagta
    27181 caaattatcc ctctggaagt ggttatccga aatattgcag caggaagttt ctcaaaacgt
    27241 ctaggtgttc ccgaaggtaa agaattcatc aaaccaatca ttgaattcta ctataaggat
    27301 gaccagttgg atgatccatt tatcaatgaa gagcatatct atttcttaca acttgcaaag
    27361 ccggaagaaa tcagtgagct gaaagcccaa gcgttggcta ttaatgaaat tttgaccgaa
    27421 ttatttgcta aggtcggttt ggttttagta gatttcaagc tagaatttgg ccggtcgaat
    27481 ggacaaattt tgcttgcgga tgaagtaaca ccggatactt gccgattatg ggatgaacgt
    27541 acgaatgaac atatggacaa agacgtttac cgcagagatc taggtgattt gatcccagtc
    27601 tatcaagaag tatataaccg tctatcggaa ctaactgaag aaaaggagcg agtatgatgt
    27661 atttcgtaaa agtatatgta acctataaag attcagtgtt agatcctcaa ggggaggcag
    27721 taaaaaaagc agtccatcgt atgggatatg aaacaatcga agatatccga ataggaaaat
    27781 actttgagat acaacttgct gatgcagaag aaccaatcga tacagtgatt gaaacgattt
    27841 gtgacaagct gcttgcaaat gtaaatatgg aaacataccg atatgaaatc aaaaaaatgg
    27901 aggaagtata acatgaaatt tgcggtcatc gtttttccag gttcaaattg tgacgcagat
    27961 atgttatggg cagttcgcga gatcatgggc gctgatgccg aatttgtacg gcatgatgag
    28021 tcctctttag aaggatttga tggggtttta ctgccgggag gattctccta tggtgactat
    28081 ttgagatgcg gtgcgattgc tcggttttct acaatcatgg aagaagtgat tcgttttgct
    28141 gaagaaggga aaccagtatt cggtacatgc aatggattcc agatattgac agaagcagga
    28201 cttttgccag gtgtactgag acgaaatgag tctttgcatt ttatttgcaa aactgtacca
    28261 ttgaaagtca accccaagac ggcctttact tcagagtatg aagccgacga aataatctat
    28321 cttccagttg ctcatgggga aggaaattat ttctgtgacg aagaaacact tcaagaacta
    28381 aaaaaaaata atcagattgc ctttacttat aatgacaaca tcaacggcag cgtggaaaac
    28441 attgcaggga ttacgaacaa agcagggaat gtgttaggaa tgatgcctca tccagaacgt
    28501 gcagtagaag acttgctagg atcagaagat gggaaacggt tctttgcttc cattttaaaa
    28561 aacttcggaa aggtgaccgt atagatgaaa acattgaaag aaccgacagc tgaagagatc
    28621 aagaaaaaaa gaatctatgc cgaatgggga ttgactgatg aagaatatcg aatgatcgaa
    28681 gaagatatct taggaagaat gcctaattat acggaaactg gactgttttc tgttatgtgg
    28741 agcgaacatt gttcctataa aaattctaaa ccggttctac gcaaatttcc tactagtgga
    28801 cctcatgtgt tgcaaggccc tggagaaggc gcaggcatcg tcgacatcgg tgatggacta
    28861 gcagttgtct tcaaagcaga aagtcacaat catccttcag ccgtggagcc ttatgaagga
    28921 gctgcgacag gagttggcgg aatcatcaga gatattttca gcatgggggc tcgtccaatt
    28981 gcgttgctag attcattgag gttcggcgaa ttagataatc caagaacgaa atatttacta
    29041 gaagaagtag ttgcaggtat cggcggatac ggcaattgta tcggcatacc gactgtaggc
    29101 ggcgaaattg catttgatcc ttgttatgaa ggaaacccat tagtcaatgc aatgtgcgta
    29161 ggtttgattg aacacaaaca tatccagaaa gggcaagccg ctggcgtcgg taattcgatc
    29221 atgtatgtag gtgcaaaaac aggtcgcgac ggtatccacg gagcaacttt tgcctcagaa
    29281 gaatttaatc aagaagaaga acagcaacgt tctgctgtac aagtaggcga tccttttatg
    29341 gaaaagctgc tgcttgaagc gtgtttggaa ttaatcttgc atcattcgga ttctctaata
    29401 ggtatacagg atatgggagc agcaggactt gtttcatcta gtgcggaaat ggcttcaaaa
    29461 gctggctcag gacttatatt gacattagat gaagtcccac aaagagaaac tgggatgaca
    29521 ccttatgaaa tgatgttatc ggaatcacaa gaacggatgt tgatttgtgt taaatcagga
    29581 gaggaagaaa aaatccaaga actatttcag aagtatgatc tagatgcagt cactatcggt
    29641 aaagtaacag acgacgggca atatcggcta tatcatcaag gagaagaagt agcaaatctg
    29701 ccagtcgatg cattagctga agatgcacca gtttatcata aagaaatgaa agaaccagct
    29761 cgaatcgtag aatttcagca gttagctcct tatcagccag ttatcgaaga acctggacaa
    29821 gtattgcttg atctgctgca gcaaccgacg attgcttcaa aacgttcaat ctacgaaact
    29881 tatgattcac aagtacaaac caatacagtt gtccgtccag gaagcgatgc tgctgtgatg
    29941 agggttagag ggacgaataa agcgttagct atgaccacag actgcaacgc gcgctatctg
    30001 tatttggatc caaaaatcgg aggacaaata gcagtggcag aagctgctag aaatattatt
    30061 gctagcggtg gtaagccctt ggcgatcacc gactgcctga actatggttc tcctgataaa
    30121 ccagaagttt tttgggaatt atcgacttca gctgacggaa ttgctgctgc gtgtgaaacg
    30181 ttaggaactc ctgttatttc aggaaatgtc tctttatata atgaaacgga tggtcaagca
    30241 atctatccaa caccaatgat tggtatggtc ggattgattg aggatcataa acatatcact
    30301 actcaggaat tcaaaaaatc aggggatcta atctatatat taggtaagac gtttgcagat
    30361 tttgatggca gtgaattaca aaagatgcag cttggaagaa tcgaaggtgt gatccgaaac
    30421 tttgatctat ccattgaaaa gagaaaccaa gagttagtat tgacagccat ccaaaacgga
    30481 ttgattgaaa gtgctcatga ctgttcagaa ggtggcttgg caattgcact ggcagaatca
    30541 gctttcaaac atcagctagg gatatctgta cagtttgaat tgtcctctgc tcaattattt
    30601 gcagaaactc aatcaagatt tgtgttgaca gtcgcaccgg aaaataagac acgattcgaa
    30661 gaaatgatgg gagatgctgc tgttttagca ggtaaggtga cggatgaagc gataatcgaa
    30721 atttcagcaa ctgacggtca gatcaaaata gaaactgcag tagcaagaaa gtgctgggag
    30781 gatgcaatcg tatgtcttat gaagtgaaga gtttaaatga agaatgcggg atcttcggtg
    30841 tttggggtca tccagacgcc gctagtgtgg catacttcgg gctccatagt cttcagcatc
    30901 gaggacaaga aggtgcaggt atcgtgtcca atgatcgagg aaagcttaag gggcatcgag
    30961 atttggggct gctttctgag gtattcaaag atcaaagaaa attgcagcag ttaacaggag
    31021 aagctgcaat cggacatgta cgttatgcaa cagcaggcaa tggaagtgtg gacaatattc
    31081 agccttttct attcaaattt catgatcagt cgatcggctt agcgcataat ggtaacttga
    31141 tcaatgctcg ttcccttaga aaagagttag aggaaaatgg ggcgatcttc cattctaact
    31201 ccgatacgga gatattgatg catttgatcc gacgaagtga agaaacagat tttttaaata
    31261 aattaaaaag tgctttgaat gaagtcaaag gtggctttgc ctatttatta atgacagaaa
    31321 cggcaatgat tgccgctttg gacccaaatg gttttcgtcc attagcaatc ggacaaatga
    31381 aaaacggagc atacgtgatt gcctcggaaa cttgtgcatt agaagttatt ggggcacgtt
    31441 ttataagaga tgtccagcct ggtgagatag tgatcatcaa tgacgaagga atccagattg
    31501 attgctttac gaaggacacg cagctttcta tttgttcgat ggaatatatc tattttgcca
    31561 gaccagattc aaacatcgca gggatcaatg tgcatactgc acggaaaaat atgggacgga
    31621 atttagcgaa agaatcgcca gtaaaagcag atatggtcgt tggcgtacct aattcttctt
    31681 tgtcagccgc aagcggttat gcggaagcta gtggaatccc ttatgaaatc ggattagtaa
    31741 aaaatcaata cattgcccga acatttatcc agccaacgcc tgagctaaga gagcaaggtg
    31801 ttcgaatgaa attatctgct gtacgaggag tagtggaagg taaaaaggtg attatggtcg
    31861 atgattcaat tgttcggggt acgacaagca gacggatcgt ccatcttttg aaagaagcag
    31921 gggcaaaaga agtccatgtg aggattgctt ctcctccttt gaaatatcca tgtttttacg
    31981 ggattgatat tcaaacgcgt aaagaactca ttgcggcaaa tcacacgatt gatgaaatca
    32041 aagaatgtat cggtgcagat tctttaggtt ttttaagtga agagggtctg atcgaatcga
    32101 tcggtctgga aaaagatgct ccgtattcag gattgtgtat ggcttatttc aacggagatt
    32161 atccgacgcc gttatatgac tatgaagaaa aatatcagga atcactaaaa gaaaaagtat
    32221 cgttctttta aaagcagacc aagaagggag tatatgaatt caatggcaaa tgcatacgca
    32281 aaagctggcg tagatgtcga agctggatat gaagtagtag aacggatcaa gaagcatgtc
    32341 aaaaaaacgg aacgtttagg tatgatgggc gctataggag gattcggcgg ttgttttgat
    32401 ttatcggtct ttcaattaaa agagccagtc cttgtctcgg ggaccgatgg cgtggggacg
    32461 aaattagcag tggcaatcga agcagacaaa cacgatacaa tcgggatcga ctgcgtagcg
    32521 atgtgtgtca atgatatctt agcacaaggc gcagagccgt tatatttctt agactactta
    32581 gcaacgggga aaaatgatcc gttacgtttg gaaaatgtag taaaaggtgt agcagatgga
    32641 tgtgttcaag gaaatatggc gctgattggc ggagaaacag ctgaaatgcc tggtatgtac
    32701 tcaggtaatg attatgatct agccggattt gctgtaggga tagctgaaaa atctcaatta
    32761 ttgacaggag aaacgatcaa cccaggagat gtgttgcttg gaatagcatc tagcgggatt
    32821 cattcaaatg ggtactctct tgttcgcaaa attttctttg agcagcagca gttttcttta
    32881 tcatcaaaac tgccagaatt gaaaaaaaca cttggcgaag aattgcttga gccaacgaaa
    32941 atctatgttt cttccttgct tccgctgatc aaagaaaaga aaataaaagg agctgcacat
    33001 atcactggtg gagggtttgt agaaaatatc ccgcgaatgt taccagaatc tatggctgca
    33061 caaatcaaac ttggctcatg gccgattctt ccaatattcg gctgtttaca aaaatatggt
    33121 gaaattcctg agatggagat gtatgaaatc ttcaatatgg gcatcggtat ggtcctggca
    33181 gtccaagaat cgaaagtaaa agaaatagta cacgaaattg agcaagctgg cgaaaaagtt
    33241 tatgaaatcg ggaaagtgat cgccaaagaa aaacatcgtg tggtgtttat aggagaagaa
    33301 cgatgagaat cgctgttttt gcttctggaa acggaagcaa ctttcaagcg ctagccgatt
    33361 atctatcgaa aaaaggattg gaatcttcga ttgactggtt gttttgtgat cagcctgaag
    33421 catatgtttt gaaacgtgca actgcgttga gtgtaccggc agattgtttt tcacctaaag
    33481 aatttgattc aaaaaaagag tatgaagaag caattttgca taaattgaaa gaaaaaaaga
    33541 tcgatttgat cgtattagct ggatatatgc gaatcattgg accggtattg ctgaaaaatt
    33601 atgataaaag aatcatcaat atccatccgt ctcttttacc tgccttccct ggtttgcatg
    33661 gcatacgtga tgcctttgaa gctggagtaa aagaaacggg tgtcacaatt cattatatcg
    33721 accaaggtgt agatactgga ccaatcatcc ggcaggaaaa agtgagaatt gaacaggagg
    33781 acacgttcga ttctttagaa gaaaaaatcc atagagtaga gcatcgaatc tatccagaag
    33841 tgattagtga aattattgaa aatggaggct atctgaaatg acaagagcat tgatcagtgt
    33901 atcagataaa actgggattg tagcattcgc ccaaggtttg attgaacaag gagtagaaat
    33961 catttcaaca ggaggaacaa agcatgcgtt ggataaagct gggatcaaaa cgctttcgat
    34021 cgaagaagtg acagggtttc ctgaaatgat ggatggccgt gtcaaaacac ttcatcctaa
    34081 aatccatggt ggtcttttag gaagacgcga tctccctagc catatggagg caatggaaac
    34141 ggaaaatatc caaccaatcg actttgtttg tgtcaattta tatcctttca aagaaacgat
    34201 tatgaaacca gatgtcgaaa tagcagaagc tattgaaaac attgatatcg gtggtcctag
    34261 tatgctccgt agtgcagcaa aaaaccatga atttgtcacc tctgttgttg atccatctga
    34321 ctatgaagaa gtactttcag aattacgtca gtatgggcat acttcacttt ctttcagaca
    34381 aaaactagcg gcaaaagtct ttcgtcatac tgcggcatat gatgcactga tcgctcaata
    34441 cttaacggat tgggtggaag aaaaagaacc ggaaaaattg acacttacgt atgaacgaaa
    34501 acaaacgctt cgttacggtg aaaatagcca tcaagccgct gccttttatc agtccgctct
    34561 tctggaaaaa tattcgatcg cagcagcaaa acaactccat gggaaagaac tttcttataa
    34621 taatatccga gatgcagatg ctgcgctacg gatcatgaag gaatatgacg agccaacggt
    34681 agtcgccttg aaacacatga atccttgtgg tattgggaca gcatctacga ttttagaagc
    34741 gtgggaagca gcgtatgaag cagatcctgt ttcaatcttt ggtgggatca tcgttttgaa
    34801 tcgagaagta gatctgccaa cagcagaaca gatgcataag ctgtttctag aaatcatcat
    34861 tgccccttct tatacacaag aagctttgga attactgcaa aaaaagaaaa acatccgatt
    34921 actgacagta gactttgcgc aggaagaaaa tcaaaatcga gaagaaacgg tctcagtctt
    34981 aggtggactt cttgtacaag atcaagatct ggcaatagaa aatgaagaag agtggaaagt
    35041 cgtgacaaaa cgtaagccga ctccagcaga agcaaaagca ttagcgtttg cttggagagc
    35101 tgtcaaacat gtgaaatcta atgcgatcgt actggcaaac gaacgtcaga cagtaggaat
    35161 cggagccggg cagatgaatc gagtcggttc agtaaaaatt gcgattgaac aagcgcaaga
    35221 gaaaatggaa ggtgcagttt tagctagcga tgcttatttt ccaatggatg attcggttga
    35281 atatgcagcc aaacatggta taaaagcaat cattcagcca ggaggaagca tcaaagacca
    35341 agattctatt gatatggcga ataaatacga tctagcaatg gtctttactg atgttcggca
    35401 ttttagacat tagagaggag tcggataaat gaacattttg gtgatcggtg caggtggtcg
    35461 tgaacatgca ataagtaaaa aacttttagc cagtccgaag gtggatacgg tgtattgtgc
    35521 gccaggaaat ccggggatga caaaagatgg tatcaattgt gtcgctatca gtgagcgaga
    35581 tcattcagcg ttaatccatt ttgttaagaa agaggctatt gattggagta tcgttggtcc
    35641 tgaagttcct ttgttaaatg ggatcgtcga tgattttatg gcggaaggat tgaaaatctt
    35701 cggtccgaca aaagcagcag ctctgatcga aggttcgaaa agttttgcta aacaattaat
    35761 ggatacttat caaatcccga cagctaaatc cagaagtttt gaatgttacg aaaaagcagc
    35821 agcgtatatc aaagaaaatg gtgcgccaat cgtagtgaaa gcggacggct tagcagctgg
    35881 gaaaggtgta gtcgtggcta ccacagtgaa ggaagcgctg gatgcggcag aacaaatgtt
    35941 gaaccagcat cgatttgggg atagtagcaa aaaaatcgtc gtagaagagt ttttggcagg
    36001 agaagaattc tcccttttgg cgtttgtccg taataccaag gtgtatccga tggtcatttc
    36061 gcaagatcat aagcgggcat ttgataacga taaagggcca aatacaggcg gaatgggtgc
    36121 ttattcacca gtaccgcaaa tttcaaaaaa gatgattgaa aaagctgtgg aagaagtttt
    36181 aaaaccagcg gctttgggaa tgaaagaact aggacgctct ttcacgggtg ttttatatgc
    36241 gggtttgatt gctacgaagg aaggaccaaa agtgatcgaa ttcaatgcgc gtttcggtga
    36301 tcctgaaacg caagtcgttc tttctcggtt gaagagtgac tttgcgcaag taatcgatga
    36361 tttattagag aatcgtacag tagagttaaa gtggcagcaa gaaggataca atgtaggcgt
    36421 tgtggttgca gctgccggtt accccgaaga atatgagaca ggaatggttc taccagattt
    36481 tctggaagaa gagctttctg tttactatgc tggagtttcc tcaaataacg gcaaactagt
    36541 tggagcagga ggacgtcttt atttagtaga agcatacggt gagacgatca aagaagcaca
    36601 gaaaaaaatt tacaccgcgt taatggataa aacgacagat ggaacatttt atcgtacaga
    36661 tattggagaa aaagctgcca atagcgaact gatcgtgaag taaatcagct gcttccattt
    36721 ataaaaaata caacaaacat aatcggaagc gccgatttta gtgaagcctt ttcataaaga
    36781 gtgtggtaca cttttgttgt gtatataaat aaggaggagt tcagatgaaa aaaattcaat
    36841 taggtacttc tgatcagaaa gcagctgcag tgatcttagg ctgtatgcgt atcaacaatg
    36901 cggaagatcc agtaaaagtg attgagacag cttatgaaaa cggcattgat ttttttgacc
    36961 atgcagatat ttatggaaac ggtgaatgtg aagaaatctt tgcagatgct ctagcaaaaa
    37021 catcaatcaa acgagaagat ctttttattc aaacaaaatg tggaatcgtt ccaggtgtga
    37081 tgtttgattt ttctaaaaaa catatcattg aatctgtaga aaaaagtttg acccgtctga
    37141 aaatggatta tgtggatgct ttacttcttc atcgtccaga tacattggta gaaccagagg
    37201 aagtagctga agcgtttgat gaattggaac ggtcaggaaa agttcgttat tttggcgtca
    37261 gcaatcaaaa accaatgcaa atcgaattgc tgaaaaaata tgttcgtcaa ccattagtag
    37321 ctaatcagct gcaatttggc ttgaaacata ctggaatgat cgatcaaggg attcatgtca
    37381 atatgacaga tgaagcaagt atcgatcatg acggtagtgt acttgattat tcccgattgc
    37441 atgatatgac gatccaagca tggtcaccat accagtatgg attttttgaa ggtgtattta
    37501 tcgggaacga aaaattccct gaattaaacc aaacattaga agaactagca gctaaatatc
    37561 aaacgacacc aactggcttg gcttctgctt ggattttacg ccatccagcc aatatgcaag
    37621 tgatcgccgg tacgatgaac gtagggcgaa tcaaggaaat cgctcaagct tcagaaatta
    37681 atatgtctcg ggaagattgg tacgaattgt accgtgctgc tggaaacatt ttaccttaaa
    37741 gattgtcata agcaggtcaa aagggctata aattaaaaag acagaataac ttgaaagctg
    37801 ttttcagctt cgagatattc tgtctttttt tattgcaaat ggaaagagta tcattgtacg
    37861 gatttcgttt attagttatt tttgttttta aggaaaagct gtgtccgata ttcttttgga
    37921 gaaatccctt gttttttctt aaataaataa ctgaaatagt tagggtcatt ataaccaatt
    37981 tcaaaagcaa tatctgaaat acgatagtct gattgctgca ataatttttt tgctaattcg
    38041 attctttgtt ctgtcagata atcgatgaat gtttggttaa acgattgaga aaaaatcgta
    38101 ctgaaatgag agggacttaa tgcaacttgt tgagcgaccc aagttaagga catatctgga
    38161 tcggtaaaat tttctttgat aaaatccaat gcatggttca aaattggctg atatttgttc
    38221 cttgaaggct gtgagggcag agcagttaag aaagaaatga acatataaac tgtcgaacgg
    38281 taaagcgagt cgtcggcgct gattgctaag cgttgactgg tggatgtgca atgaaccatt
    38341 tcttgcggga ttttcttttc tttcaggcag acatgctgga gtagcttagt caagacaaat
    38401 aaacgatata atcgtgttcg agttggtgtg ccttgtggtt tcgtcaagtg ttcgatgtac
    38461 ttagatactt cgtttttcga taatgaagac aaatcaagtg agaggtcaaa gtaaaagaca
    38521 gaatcaactg gaagtttcgc ttcttgaatc aaatcctcat agctgataat ttgttcttta
    38581 agggaactgg cggtttcaag tatcatctgc gtgatctcaa aagatgcagg taattcgctg
    38641 attctttctg cgacaggacc aatggcgaca gaaaaattat ttttttgtcc cttttctaat
    38701 tcatggacca acgtttgggc tacctgataa ctggtctcga tcacattttc ttcaagagaa
    38761 tcaactatta aaaatttgat gaatcgagag gaaacagcag agaagatcac ttgttgattc
    38821 tccctaaata aaaacttcaa ataatcactg agatgagcgt aatcttcaaa gtctgtactg
    38881 acttggtttg ctgctaataa tacggtcatt tttttccctg ctaaagagcg attgaaagat
    38941 tcagcaagtt tcaatgtttc actgatcgta agtgagcctt tgaacaaacc gttcaagaaa
    39001 tgattttttt tcaattcgaa aacaagatca gaagaagtgt gcgtttcttt ttgctgcaaa
    39061 tctaaagcag ttactgcttt ttctattgct tgtttcaatt cgctactttt aatcggttta
    39121 agcagataat catctgcctg tacctggatt gctcctttca tataatcgaa atcgtcgaat
    39181 ccactaataa atatgagacg agtccaagga agtagtttcc ttgcttcttt tgaaaaagtc
    39241 aacccgtcca tgaaaggcat ttggatatca gtcagaatga tgtcgggctg taagtcgata
    39301 attgaagaca gtgccatctc accatctcca gcttcgccga cataagtgat tggataataa
    39361 gaaccaagga cagttatttg ttcccgcaag ttattgcgaa tgaggtattc gtcttcgatg
    39421 ataaaaactt tatacatgtt tgtttcctcc aattgttttg ggagatagag ggatcatcag
    39481 tgtgacagtt gttccttctc ggtatttgct gtcaatcgtg ataggttttt gatcatttcc
    39541 gtaataaaga aggagacgct tgtttacgtt gtaaagtccg tagccgatac tgaaatctga
    39601 atcgatccct ttggctaact ctttacgtat ttctgccagt ttttctgcag ggatgccaat
    39661 cccgttgtct ataactttga attggatcat ctcatcaatt ttgcatgtgg taatacgaat
    39721 aaatcctttg tgacggacgt acttcgttcc atggtataca gcattttcaa tcaatggctg
    39781 caagatcatt tttaaaatag aaacaggaaa aagtgtttct ggtatatcga tcttatagtc
    39841 aagcatatgg ccgtatctta tttttaagat gactagataa tcattgatat ggctaatttc
    39901 tttttcaaga ggaatccagt cttgtccttt gcttaaagac gtacgaaaaa agccggaaag
    39961 tgcatgggtc atagtcagag cttcttcaaa gcagtcttgt tcgatcaaag aaataatggc
    40021 atccaaactg ttataaataa aatggggagt aatctgagct tgcaatacac gaacttcact
    40081 ttgtgctaaa tggtattgtt tcaaagcgtt ctcccgtaaa gactggttta gatcttccgc
    40141 cattttattc atgctagcgg taagtgtttg taattccagt gtttccggca gggttaaacg
    40201 aaagccaagg ttccctgaag aaagttgttc tgacatctcg atcagctgat tgatcggttc
    40261 ttggaccttt ctctctaaaa agcgacgatt ctttttcgca tagaaaaaaa tggtactaag
    40321 aactccaagt tccactattg ttaatagaat gagcgagcgg acaagtgtcg tatttttttt
    40381 gcttgctaag tcgatctcca cacgaacaaa atcttgtaag atatctgaca gcagttgagt
    40441 gacagaatct acctgattga tgatctcttc attggtatcc ataaaatctt ttgccaagat
    40501 attattgatc agctcgtttt gataatcttc cagtgtatcg atcgtacgta gcgatacatc
    40561 taacgtacta atttctttaa gtgtatttgt attatttttg atctctcgaa tatcattcct
    40621 aagttcttct aggatatttt cctcattgaa ctcatctacg gttacttggc cagaaatcaa
    40681 gtcccacata ctttccaaaa ctttttgatc aaccttcaaa gcaattgcat ctgctttttg
    40741 gatatttgcg atcgtctgtt gataaatcaa aagctgttgc gaatataaca gtatgctcag
    40801 tagcaaaggg ataacagcaa atagaagcat ggctttgttt gcttgggcaa aaatctgttt
    40861 tatgcttaaa ttttttgtca tgatacttct ccagtcaata atttctggtt gggatgttag
    40921 ctatattctg ctgggaaaag acagtctcgg aaataaaaat atctttaggt aatgtttttc
    40981 ctgtttgaat atttccaaaa taacgagtaa cggtattggc tacataccag ccgacatcag
    41041 gtttacactc gactacacaa ttgacgattc ctgagcgtag cttctcgatc atatcttctt
    41101 gtccatcaat agttacaatg atcaaatctt ctccaggttt gattgatgtt tcttttatcg
    41161 cagctaaagc accgtgagtc atttcatcac tatggctgta taatacatcg atttcggata
    41221 aatcatttgt ttctaaatat cgtttcatct ggtcttttcc tttcatccga ataaaatcac
    41281 cgacaattcg gtgaacaata gtcatcggag gtttatggtc gatggttcca tcttttttta
    41341 gggttggttt acgattgatt gattccaaaa atccttcgga gcgtagcttg gtcggtgtgg
    41401 agttctctga accagcaagt tctaggattc tgatttcctg tttctttgaa tttgtgaagt
    41461 gattgctgac aaataaaccc gcccgatttc cttctgcttt gaaactgggg ccaatgtggg
    41521 tgacgaaaag ctcgggatca ttcgtttgaa tatgtctgtc gacaagtatg acagggatcc
    41581 ctgctcgctt tgcttctttt aatacagagt cccacccgga ttcttgcaaa ggagtaaaaa
    41641 tgatcatatc cactttatag gcaatgaacg tccgtatatc ttggatttgc cgttcctgat
    41701 tcatgtagcc atttcggtag agaacttggt aatcttcttt ttcaagcgca gtgataattg
    41761 attcagtatg ctttttccgc cagctactct ccgttcctga ttgcgaaaaa ccaacgacta
    41821 gtgggtcagg tgcttccttt gtattaggtg tacaccccat catttggtaa gcgattacaa
    41881 caagggctaa aaaaaataaa acacgtttca ctttaaatca ctccatctgg atttgttagt
    41941 actatcttat cagataaaag ggaaaatcaa agtcaaaagg aatcaatcga aaaaaatcca
    42001 agaaacttaa aaaaatccaa gacaaaaaaa gtacacctaa aaaatttaaa gtaaatccca
    42061 taaaaaaatc aatgttaacg ctttctttat tagctatact cggtgtatca attgaaatgg
    42121 gggataaaaa atgaaaaaca aatttagaaa gattgttggg actatcgcta ttttgaccgc
    42181 aagcattcta ttaagtgcgt gtggcaattc aggatctgca gatgatagta cggggtatgt
    42241 aggaatcgct atgccgacca aatcagcaga aagatggatc gctgatggga acaacatggt
    42301 ctcggaatta gaaaaactgg gttataaaac agatcttcaa tatggagaag ataaagtaga
    42361 aaatcaagta gcacaaatag aaaatatgat tacaaaaggg gtagatacac ttgtgattgc
    42421 ttctatcgat ggttcggctt taactgatgt gttagctaaa gcaaaagaag cagatatcaa
    42481 ggtgatcgcc tatgatcgtt tactgatgaa ttcagaaaat gtcgattatt acgcgacttt
    42541 tgataatttt ggagtaggtg tatcgcaagc agcttatatt gaagagcact taggattgaa
    42601 agaaggaaaa ggaccattca cgattgaatt atttggtggc tcgcctgacg acaacaacgc
    42661 actaatcaat tacaacggag tgatgtctgt tttacaacca tatatggaca acggtcagtt
    42721 ggtcgtgcca tctggacaga cgagtttcag tcaaatcgct acgcttcgct gggatggttc
    42781 tacggcgcaa gcaaggatgg ataatctgtt aagcgcaaat tatactgacc aaacattgga
    42841 tgctgttttg tcgccatatg atccaataag tttaggaatc atttcgtcat taaaaggcgt
    42901 ggggtatggg agtgaaagta aacctttacc tgtgattact ggacaagatg cgacagttgc
    42961 tggcgttaaa tcgatcattg ccggggaaca aacacaaacg atctttaaag acacacgtat
    43021 cttagcaaaa aatacgattg aaatgatcaa agcaatcagt gatggagaag aggttccggt
    43081 caatgatact gaaacttatg ataatggggt gaaaactgtc cctacgtatt tagcaaatac
    43141 agtatctgtc gataaagata attatcaagc agagctgatc gacaccgatt attataaaga
    43201 atccgatctg aaaaattaag aggaaccaaa caaggaacta aagagatcta agtcagagag
    43261 aggataagaa ttatggcgga ttgtatttta aaaatggcag atatcaccaa aaaattttct
    43321 ggaatccgag cgttagattg cgtgaatctg atgattgaac gtggggaaat ccatgcattg
    43381 tgcggagaaa acggagcggg aaaatcaaca ttgatgaatg tcttgagcgg cttgtatcca
    43441 tatggctcct atgacgggga aatcttttat gatggaaagc tgtgtcaatt taaagatttg
    43501 aaagatagtg aagaaaaagg catcgtcatc attcatcaag aattagcact aagtccttat
    43561 ctttcaataa aagagaatat ttttctcgga aatgaacaag cagaaagagg cgtgattgat
    43621 tgggaactga cagaaaagaa aacatatgaa ctgctgaata aagtcggttt gtctatcgac
    43681 ccgaatcttc tagtatccca gattggcgta gggcaacagc aactagttga aatagctaaa
    43741 gcattatcaa aatccgtccg tctgttgatt ttggatgagc ctaccgctgc attgaatgaa
    43801 gaagaaagcg cgaatttgct gagattgatc cgagagttga aagagcaagg tgtaacctcc
    43861 atcattattt cacataagct gaatgaaatc gtggatatcg cagatcgaat caccatttta
    43921 cgggatggac agacgattga gacactagaa aatgaaatga tcagcgaaga gcatattatt
    43981 cgcggaatgg tgggcagaga tttagttgat cgttatcctg aaagaaaacc ggatattgga
    44041 gaagtttatt ttaaagtaga agactggacg gtctatcacc ctcttgatca cgagcgaatc
    44101 ataaacgacc acatcaactt gcatttgaaa aaaggagaga ttgtaggaat agctggactg
    44161 atgggagcgg gcagaactga attcgctatg agtctatttg gacattctta tggaagcaag
    44221 atatccggac gtatattaaa ggaaggggca gagatatcag ttaagaccgt tccttcagcc
    44281 attgcaaacg gactcgctta tgtctcggag gaccgaaaat ctttaggact gaatctgctg
    44341 atggatattc gggaaaacgt gacgctgagc agtttggata aaataagtca aaaaggagta
    44401 ttagataaag aaaaagaagt aactgaggca gaaaatttcc ggaaaaagat gcgtataaaa
    44461 acaaactcca tccatcaaat tgtcagtagt cttagtggtg ggaatcaaca aaaagtcgtt
    44521 ttatccaagt ggctgatgac acaacccgat attctttttt tggatgagcc aacccgtggg
    44581 atagatgttg gggcaaaata tgagatctac acgattatcg aagaaatggc agcaaatggc
    44641 aaagctgtct gtcttatctc ttctgaactt cctgaaatat tagggatgtg tgatcgaatc
    44701 tatacgatga acgaaggacg tattacagga gaagtctcgc gagaagatgc agatcaggaa
    44761 gtcttgatga aattaatgac gaaagaagag gtgggttaag ttggaacagg caaaaaatct
    44821 tacggataaa aaagagaaaa aactaaattt gaaaagtaga gcagctgatc tttttggtcg
    44881 ctacagcatg gtgatcattt tagcgggact acttattgcg tttcaattga tgacggatgg
    44941 tattttctgg cgtccattga atataactaa ccttgttctg caaaatagcc atatattagt
    45001 actagctgca gggatgttat tagttgtgct cttaggtcat gtggatttgt cggtagggtc
    45061 agtgatggcg tttgttggtg caattgcggg catgatgatg gtcaatcgcc aaatgaaccc
    45121 gtggatgaca gttatcgcat gtcttgcagt aggcgggctg atcggcgcat ggcaaggttt
    45181 ttgggtagct tatgcaggga taccggcgtt tatcgtgact ttagcaggat tgctgatgtt
    45241 tcgtggactg acacaagttg ttctaggagg ccaatctcta gcaccttttc cagctgtttt
    45301 ccaaaaaatg tctacgggtt atttaccaga cttaagcgaa ggaagcagcc ttcacttctt
    45361 gacattgatt ttaggaatga tcatcacgat cgtgttagtt gttggtcaat ggcgaacaag
    45421 aaatcgtcgg aaaaatcatt tatttgaagt ggaatctcca acagctttct tggtaaaggc
    45481 ggtatttaca gcagtcgttg tactaggaat aacttatatc tttgcctctt atcaagggta
    45541 tccgattatt ttgatcattc ttggtttgat cgttgcagca tatgggtttt tgacaaataa
    45601 aacagtagca ggtcgacaga tttatgcgac gggcggaaat cgaaaagcgg ctgaactatc
    45661 agggatcaaa acaaagcgaa tcacgttctg ggtattcgtc aacatgggag tcatggcagc
    45721 tttagctgga ttagttttag ctgctcgatt gaatgcggca acacctcaag caggtacatc
    45781 gatggaactg gatgcaatgg cggcagtata ttttggtggt gcttcgactt ccggcggcat
    45841 tggaacaatc atgggtgcga ttgtcggtgg cttggtcatg ggtgtattga acaatggcat
    45901 gtcgatttta ggcgtcgggg tagactggca gcaggcaatc aaaggattga tcttgctttt
    45961 agccgttgtg ctagacttgt ataataagaa aaaacgaaca acgtaggaga acgccggtta
    46021 ttcagaaaaa agactgtgac aagacatttg tcacagtctt tttttcgcta aaagcgaaca
    46081 ttcattgttt cgtttgtata gactaaaaat aatatatgtt tttttaccat tcgtataaat
    46141 tctctttttg ctcttgattt ttttgcttaa ttgagttaaa ttacataggg cgctaaaaaa
    46201 agccttgatt aaattaataa tgttcgtgtt ttgctggatg tgaacatcag gaggataatg
    46261 taatgaaagt atttgattac gaagatattc aattaattcc taataaatgt atcgtaaata
    46321 gccgttctga atgtgataca acggtcacat taggtaaaca tacattcaaa atgccagtag
    46381 tgcctgccaa catgcagacg attattgatg aaacaatcgc agaattctta gcggaaaatg
    46441 gttatttcta tatcatgcat cggtttgatg aagcagctcg aattccgttt atcaaaaaaa
    46501 tgaaaaagcg tggattgatt acctcgatca gtgttggcgt caagaaagag gaatattcct
    46561 ttatagagaa gttggcagaa gaatcgctaa atcctgatta tatcactatc gacatcgcgc
    46621 atggacatgc taactcagtt attgatatga ttcagcatat taaaaaatat ttgcctgaaa
    46681 cttttgtgat tgccggtaat gttggtacac cggaagccgt ccgtgaattg gaaaatgcgg
    46741 gcgcagatgc aacaaaagtc gggattggtc caggaaaagt atgtattaca aaaatcaaga
    46801 caggctttgg gactggaggc tggcaactgg cagctcttag atggtgtgcg aaagcagctc
    46861 gcaagccgat catcgctgac ggtgggatca gaacacatgg cgatattgca aaatctgtcc
    46921 gttttggtgc gacaatggtc atgattggtt cattgtttgc cggtcacgag gaatctcctg
    46981 gcgaaacaaa agtcgaaaat ggaatcgtgt ataaagaata ctttggttct gcttcagaat
    47041 tccaaaaagg cgaaaaacga aatgttgaag gaaagaaaat ctggatccaa cacaaaggat
    47101 ctttgaaaga tacactagtg gaaatgcaac aagaccttca atcatctatt tcttatgctg
    47161 gaggaagaga tctggaagcc attcgaaaag ttgactatgt catcgtgaaa aattcgattt
    47221 ttaatggaga cacaatctaa aaaataaata aaatattagg atgttgtgaa aaagttgtcc
    47281 tgcattaagt aataagaaca gccaaacaaa aatagttctt tatatgtttt gtttggctgg
    47341 aattattatc gcagatacaa gcttttgaac atcattattc agaataaggg actgtgataa
    47401 gatttttgtc acagtccctt atgttttgca aaaaaagttt ttcttttttc tcgtaaattc
    47461 ggtaaaatca aaagaaaaaa tagacaatag aaaagagaag cttatgtggg aaaaagtaga
    47521 gaaaaaacaa ggaccgattt atcgtcaaat tatggaacag attatgagag aaatcgaaaa
    47581 cggaaggatc aatcctggaa gtcaacttga atcagaacga aaactagggc aactttttgg
    47641 tgtgaatcgt tccaccgttg tccatgcatt ggaagagctt agggagcttg gggtactaac
    47701 gagcaaacgt ggcagcggat ggtttgttaa tcagactgaa tgggggaaat tcgctgttcc
    47761 aagaatcgat tggcggcaaa tgatctctcc tcgttatgag cagacggact ggtatgagca
    47821 aaaaataaga gaagcaaagc agatccaaaa aacatcgttt cttgatttat acgctagcga
    47881 gatgccagat gagcttttgc cgaattttga gtttccttct tattctcttg aagaaatctt
    47941 agcggaagaa aaagaaatgg atcttttagg ctatactccg ttacgtaaca aaatcaaacg
    48001 gtatttggaa agtaaatttg aattggcatt ttcccccaac cagcttcttg tgactggtgg
    48061 cggtcagcaa gcaatctttt tgattttaca aacgattctt tcagctggag aggggatagc
    48121 tgtagagtca ccttcttttt tttaccgatt acctcttttc aaagcttcag gaacgcgttt
    48181 atttgggata ccgatggatg aagaagggat cgacttagaa aaactgaaag aatcgatcca
    48241 taaaaataaa ttgaaagcag tgcttatcaa cccgaatttt caaaacccta caggaaaagt
    48301 gatgagtcaa aaacgacgag aaaagctagt agaattgtgt cgacagtatc ggcttccaat
    48361 tattgaagac gatgtattta cggatctttc atttctaaac caagaagaag tccttcctat
    48421 acgaacgatt gatccggata atgtgttata cgtcggttcg ttatctaggg ttttaggaaa
    48481 aacaacgaaa atcggttggg tgataggacc tgagactttt atctcgcaac tagctcaagc
    48541 gcaagaaatg atggagtttt cgatgaatat ccttacacaa atcacagtgg ctaatgtatt
    48601 taatgaagca attgatttga aaatgcaaga tgtaagaaaa aagttgaaag aaaaaagccg
    48661 actgataagc gcttggggag attcacaatc gtttttcaag cttcattcaa ttttaggtgg
    48721 ttactatgcc tggattactt gggatgggaa aaaactagac cccgaactag cagaaaaagt
    48781 agtagatatg ggtcttggaa ttgcaccaag tctacttttt gggcaagata taaatggaat
    48841 cagaatcaat tacagtaggc tggatgaaaa ccaaataccg ttgttttatt caaagatgaa
    48901 aattctagaa aagtggttaa cagaataatt tcacaaagta aatagattaa agaatataac
    48961 agcttggatt taaatttcaa gctgtttctt ttttttcgga aaaagaatac gatataaaga
    49021 aactaagaaa aaaccattat ttgtacgtcc gttcacgatg tgtaaataaa ataaatgttt
    49081 gcaacaaaac tgtataacac caacgttttt aaatagaaac agatggttgt aagtttgatt
    49141 agaaaataga aaaaatgcag atgaaatata aaacgctttc cttattgtga ttttattcac
    49201 taatgctgtt aatatgcgga ttggcataat ttcctttcat cgtgaaaatt tgtgctttta
    49261 gcttgtattt ctcataaatc catgataatt tattaatgaa gacggttaaa tagtgataca
    49321 gttttattca ctgtttcaat cgcagaatga agagattttc ttatagatga gagaggaatg
    49381 tgtgacaaat ggcaaacaaa aaagcaccga tcgacttcca agcactgcta gatgaagtca
    49441 atgcagattt cccagtatat caagcacttg atcaagatgg aaacgtagtt aacaaagatt
    49501 tagtacctga tttatccgat gatgaactcg ttgaattaat gactcatatg gtatggtctc
    49561 gtgtattaga ccaacgttca acagcgctga accgccaagg acgcctaggc ttttttgcac
    49621 caactgctgg acaagaggca agtcagctgg ctagtgctta cgcatttgat aaagaagatg
    49681 ttcttcttcc aggttatcgg gatgtacctc aactagtaaa acatggttta cctttatctc
    49741 aagctttttt atggtctcga ggacatgctg caggtaattc ttatccggaa gaattgaaag
    49801 ctctaccacc acaaattatc atcggtgcac aatatgtaca agcagccggt gttgcattag
    49861 gtttgaagaa acgaaacaag aaaaatgtcg tcttcactta tactggtgat ggcggttcat
    49921 cacaaggtga tttttacgaa gcaatcaatt tcgctggcgc ttaccatgca aatgccgtat
    49981 tttatatcca aaacaacggg tatgcgatct ctacacctcg tgaaaaacaa actgcagcaa
    50041 aaacacttgc acaaaaagct gtcgcagctg ggattcctgg tattcaagtc gatggaatgg
    50101 atccgcttgc agtatatact gtatctaaaa tggcacggga atgggcgatc agtggaaatg
    50161 gaccagtcct aatcgaaaca ctgacttatc gttatggacc acatacacta tctggtgacg
    50221 atccaacccg ctaccgtaca aaagacacag atgacgattg gcagaaaaaa gatccattga
    50281 tccgcttccg taaatatttg acggaaaaag gactttggtc tgaagaaaaa gaagaacaag
    50341 tcatcgaagc aactaaagaa gaaatcaaag cagctattgc tgaagccgac aaagtaccaa
    50401 aacaaaaggt ttcagatttc ttgaaaaaca tgttcgaagt atctccacaa tcaatcaaag
    50461 aacaaattgc aatttatgaa gcgaaggagt cgaaataatc atggcacaaa aaacaatgat
    50521 ccaagcaatc acagatgcct tagcacttga actagaaaat gacgagaatg tcgttgtttt
    50581 tggtgaagat gttgggaaaa acggaggagt tttccgggca actgaaggat tgcaggaaaa
    50641 gttcggagaa gaccgagtat tcgatactcc tttagctgaa tctggaatcg caggattgag
    50701 tttcggtctt gcgttagaag gatttcgtcc agttcctgaa atccaattct ttggttttat
    50761 tttcgaagca atggacgaag tagtagctca aatggctcgt acaagatatc gaatgagcgg
    50821 cactagaaat ctacccatca caattcgttc gccatttggt ggcggtgttc atacaccaga
    50881 attgcattcg gataatttag aaggtttgat cgctcagtct cctggtattc gagtagtgat
    50941 tccttccaac ccttatgatg caaaaggact attgatttct tctattagaa gtaacgatcc
    51001 ggttgttttc ctagaacaca tgaaactata tcgctcattc cgagaagaag tgccggatga
    51061 agcttacgaa gttcctttgg ataaagcagc agttactcgt gagggaacag atgtatctat
    51121 tatcacatac ggagcaatgg tgcgtgaagc aatcaaagca gcagacaatc ttgcaaaaga
    51181 aaatatatct gttgaaatca ttgatcttcg tacagttgcg ccattagatg tagaaacgat
    51241 cattcaatcc gtggaaaaaa ctggtcgcgt agttgttgtc caagaagcac aacgacaagc
    51301 gggcgttgcc gcgcaagtag tttctgagat ctcagaacgc gctatccttt cattagaagc
    51361 cccaatcgga cgagtttctg cacctgatac agtcttccca tttggacaag cagaaaatgt
    51421 ctggttgcca aatgcaaaag atatcgaaga caaagtaaaa gaaatcgcgg aattttaaga
    51481 aaagaaggga cgaaacaaag tcatggctta tcaatttaaa ttgccggata
//
LOCUS       DASHHB010000013        49996 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.013, whole genome shotgun sequence.
ACCESSION   DASHHB010000013 DASHHB010000000
VERSION     DASHHB010000013.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 49996)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 49996)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..49996
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.013"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1008)
                     /locus_tag="U2K71_000864"
     CDS             complement(<1..1008)
                     /locus_tag="U2K71_000864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM7314970.1"
                     /translation="MKKKSIILAATSALAVLTLAACSGDTNKDIATMKGGTITVSDFY
                     DEAKLESSNQSLVQRMIIYKVFNNKYGDKVTDKQVDAEYDKQAKSLGDTFESQLEAAG
                     YTKDTYKEYIRNNLAFEAGLKAHVDITDDDLKTAWKSFHPEVEAQIIKLSSEDEAKDV
                     KKSADDGDDFSKLAKDKSTDTETKEDGGKVKFDSTTTTIPAEVKEAAFKLKDGEISDV
                     ITTTNPTSYATEYYVVKMVKNQNKGNDMDKYKDQLKDIATETKLSDNAFTTKVIGEEL
                     KDANVKIKDDAFENVLSAFTTTSSSTKDSSETTASTKSSDTKSTDSTKESSTEETTDS
                     SK"
     gene            complement(1188..1532)
                     /locus_tag="U2K71_000865"
     CDS             complement(1188..1532)
                     /locus_tag="U2K71_000865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEM7314971.1"
                     /translation="MIKNFFKGLLFGAAAGTVGGLLLAPRSGNETRKKLIEELEEATT
                     LTNDLNNSLNHFKSALIETKETAESVIPAFQEAIKKDIEDFKFQAEPRITQINEQVEK
                     LKADLPEYPEQI"
     gene            complement(1525..1959)
                     /locus_tag="U2K71_000866"
     CDS             complement(1525..1959)
                     /locus_tag="U2K71_000866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HEM7314972.1"
                     /translation="MENCIFCKIINQEIPSYKIYEDDKVYAFLDISQATKGHTLVVPK
                     RHVADIFEYDPELAGEVFSRVPKIAQALEKAFPEMKGLNILNNNRELAYQSVFHSHIH
                     LVPRYSKEDDFSIHFVNHQDSYGSEELKAIQETIVKQVSCDD"
     gene            2097..2834
                     /locus_tag="U2K71_000867"
     CDS             2097..2834
                     /locus_tag="U2K71_000867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7314973.1"
                     /translation="MSLIIDHVTGGYGHLPVLKDISFDVQPGEIVGLIGLNGAGKSTT
                     IKHIIGLLEASKGTIEINHLSLKKNTEEYRKQIGYIPETPSLYEELTLREHIEVTAMA
                     YDIPMDIAMKRAEKLLETFRLSNRLDWFPANFSKGMKQKVMILCAFLIEPSLYIIDEP
                     FLGLDPLAINALLELMVEMKKEGAGILMSTHILATAEKYCDRFVVLHNGEIRADGSLE
                     MLRKEFQLPDSSLDEIYIALTKEQAHE"
     gene            2827..4038
                     /locus_tag="U2K71_000868"
     CDS             2827..4038
                     /locus_tag="U2K71_000868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7314974.1"
                     /translation="MSSFFQKRLARHQKKMMKYMRYVLNDHFVLVCLFLIGGVGLYYS
                     NWLKTLQPPFAIGGLIISVFWMLCLFVGKMATFAEAADIVFLLPKEKEMQTYLERAFR
                     YSCIFPLIFLILACGLAMPLVVVSTEQSFSMFFVYLIILWSLKFSHLQIKRAELFRLD
                     QQVIRYWKLGWFFSSLIFLFLSFYWLIWAGIAGALVQASLYYWFLWKQQEKGLDWEKM
                     VHVEEHRLHQIYQFINLFTDVPEITAKIKRRRYLDPILTHIKRESKNTYLYLFARRLA
                     RGSDFSGLYFRLVIIGGLLIAGVVDFYFSLIIGFLFLYLIGFQLLPLANQFRYMVLVQ
                     LYPIHKNQKKQAIQYLLTWVLIIASVIFGLIAAVVLDGQQRWVPLAAYLIVTMLFVKL
                     YVPTRLKKMSD"
     gene            4242..5024
                     /locus_tag="U2K71_000869"
     CDS             4242..5024
                     /locus_tag="U2K71_000869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase family protein"
                     /protein_id="HEM7314975.1"
                     /translation="MMEFQLDQEWRLRPIKGDTGKTYIGMRDEDKVFIKRNTTPMLAA
                     LSREGITPKLVWTKRTGNGDTLTAQEWLEGRLLTPNEIGQRNDVIDVLYHLHHSNSLK
                     TMLTRIGGRVMTPEKMLQKYEKELPEELQKNQFLQIVHRFLREQIPSIDKENLSVVHG
                     DVNHRNWLVCKNYLYLVDWDSIMFADPAVDIATILGNYVPLSSWNQWLISYGIRPTDE
                     MLEKFHWYAAMNLLQEITRYCKREDHRRMNEEILQLKRIFSG"
     gene            5094..5756
                     /gene="trmB"
                     /locus_tag="U2K71_000870"
     CDS             5094..5756
                     /gene="trmB"
                     /locus_tag="U2K71_000870"
                     /EC_number="2.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(46)-N7)-methyltransferase TrmB"
                     /protein_id="HEM7314976.1"
                     /translation="MRVRNRPGAAEMLAAHPNFVISDPTLWKGKWNELFENDHPIHIE
                     IGMGKGQFITGMAKAHPEINYIGVEMQVSVVSIALDKLIEQPLPNLKLLHVDGSALTE
                     YFADSEVDQIYLNFSDPWPKKRHEKRRLTYKTFLAVDEQILRPNGEIHFKTDNQGLFE
                     YSLASFSQYGMILKQVWLDLHQSQFEGNIMTEYEEKFSSKGQRIYRVEAQFQDKTKKK
                     SL"
     gene            complement(5854..7608)
                     /gene="ade"
                     /locus_tag="U2K71_000871"
     CDS             complement(5854..7608)
                     /gene="ade"
                     /locus_tag="U2K71_000871"
                     /EC_number="3.5.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase"
                     /protein_id="HEM7314977.1"
                     /translation="METTLRKYIEVASKKKPADLVIKHAKIVNVFTKEIMEKDVAICE
                     GMIVGIGEYEGKSVHNANGQYLVPGFIDGHVHIESSLLSPSEFSKVSLLHGVTTVVTD
                     PHEIGNIAGSTGLEFMIEDARQTLMNIFFMLPSCVPVTPFETNGANLNAKKLAPFLQK
                     PEVLGLAEVMNYQAVANNETDILEKIQLMHQHKKKIDGHAAGIGMEELNVYPAAGIRT
                     DHEATTAKEAKERLDLGMYLMVREGTVAKDLASLLPAITPENSRRCFFVTDDKLINDL
                     VKEGSIDHIVRQAIALGIDPLQAIQMATLNAAECFDLKTLGAIAPGYQADFFLTDDLT
                     KLPIHEVFYKGTLVVQSGQLQDHLFADHSNSYTCCLPKLNAQPLNPHSLELLLSSSQA
                     HVIEIIPNSLVTKDLVEKVDRQGERFIPSIEKDQLKIAVIERHHQTGNIGLGIVKGFQ
                     LKQGAIATTIAHDSHNLVVVGTNDEDMLYAANLLIQKGGGMTVVNNQQEIACVSLPIG
                     GIMTQEPFSIVYEQLNELITKAHQLGAAKTFDPFLTLSFLTLSVIPELKITDKGLFSF
                     SRFQLIPPCGKTDEINTF"
     gene            7740..8669
                     /gene="tadA"
                     /locus_tag="U2K71_000872"
     CDS             7740..8669
                     /gene="tadA"
                     /locus_tag="U2K71_000872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly complex ATPase component
                     TadA"
                     /protein_id="HEM7314978.1"
                     /translation="MEIKRLSDQMISESFAKHAQDVYILPASEVFYIYFRYGKKRTFW
                     KKVSSADGQQLISRFKYLGQMDIGEKRKAQLGAFTYSLEKQEVRLRLSSVGDYLQRES
                     LVIRLLYGTFETPHRCFSSRDFATILQKTVRRGLYLFSGPVGSGKTSLMYRLALEEKL
                     QVITIEDPVEIEEPFFLQLQINPKIAQTYDTLLRLALRHRPDLLIIGEIRDESTAHAA
                     VRAALTGHRVFATVHARNLEGTLVRIHELTKKKEELSECLAGVIYQELLEDANHHPSV
                     LWSYQFMEEESVKKSWEESYEEAQENIWQSELF"
     gene            8626..9675
                     /locus_tag="U2K71_000873"
     CDS             8626..9675
                     /locus_tag="U2K71_000873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein ComGB"
                     /protein_id="HEM7314979.1"
                     /translation="MKKLRKIFGSQNYSKGLTRSQQNQFAQTMGELLLSGFSLQESLY
                     LLLTIRSFPPVILANIQHQLKEGQAFSEVLSQIGYGAEQLVQIELAERHGNLPTTLLE
                     IAKQFRLVEGFRNDLKKLLAYPMLLLTFLLGILFSMRLFVLPQLIGSGMVMQEHWGIQ
                     LIQVFHWYVLLFILAVTLLALLVRYRLMKLSPVDQSEWISRQLLIGPIYSLYQSAYLS
                     LEIGKLFHEGFELRQIIVCLKETKQESLIQHLASKLIQGLEAGMSLAEQFQNYRFLSE
                     EFSKIVLQGEAKGSLGKELIFYSEWTRKQLFKRLHHWMHFIQPIIFLGVAALILLVYA
                     AVLLPVYGSIEEVLP"
     gene            9672..9950
                     /locus_tag="U2K71_000874"
     CDS             9672..9950
                     /locus_tag="U2K71_000874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin-type N-terminal cleavage/methylation
                     domain-containing protein"
                     /protein_id="HEM7314980.1"
                     /translation="MKKRKISYYSGFTLIEMLIVLLIISVLVLLFVPNLSRYRNHVDQ
                     ESREAIIQLVDTQKELYALQNNGRVPTVEELLNEGYIKREHAEIYQRP"
     gene            9925..10395
                     /locus_tag="U2K71_000875"
     CDS             9925..10395
                     /locus_tag="U2K71_000875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system protein"
                     /protein_id="HEM7314981.1"
                     /translation="MQKSISGLEGKVLASGYTLFESLLLILLLTTILSFPVLAFSAWK
                     NELAVYQFFGQFEKRIYSTQKIAIVNQIQTGFYWNSEENQIIFHVPNPDKINWEVLDI
                     PDEITLKRHASITFAARTGNESSLKAYQFYWEEKERTITYQFQIGSGRYTKRIE"
     gene            10412..10714
                     /locus_tag="U2K71_000876"
     CDS             10412..10714
                     /locus_tag="U2K71_000876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314982.1"
                     /translation="MESLTALMIASVCVYLVSFGQLFLLQAEKKSGEELLFIRMIYED
                     IKTQRMYHKRPERREEMIPYDIQIVDNNGVIEKVFVNMEKEAISIEKTSNSSIYAD"
     gene            10731..11102
                     /locus_tag="U2K71_000877"
     CDS             10731..11102
                     /locus_tag="U2K71_000877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComGF family competence protein"
                     /protein_id="HEM7314983.1"
                     /translation="MILSSLLLTFNLLIQQTIQVSEFLQRKDQKEWLIFLAQLEEELK
                     SSHNVSVKNQQLHYTIEDKQYIIERYQAMIRKRRTSGGHQPMLTSITELQLLEKEHTI
                     SFYVHFENGEDGYAIWTKNDQ"
     gene            11074..11433
                     /locus_tag="U2K71_000878"
     CDS             11074..11433
                     /locus_tag="U2K71_000878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314984.1"
                     /translation="MPYGQKTISRKCYKGSILFSVLLLFLLCTFLLMAALESYRLSMD
                     LNIRIKNYYIAKIMKQMALSEQSTLAESSEGVFYYTTGSVTYQWVQQSLFLEVEVSPF
                     IFRFTEEVKNDTDTSTE"
     gene            11605..12612
                     /locus_tag="U2K71_000879"
     CDS             11605..12612
                     /locus_tag="U2K71_000879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM7314985.1"
                     /translation="MLPEKMEQGFNQNLEAIQLLQNALGTSFLEAYVENAENLIDDYQ
                     VRVVDGVPTKETTQRITALYEELKKLSFEPEEWRRLSQLLLLKGSQTEHLQANHQLTP
                     DSIGFLFVFLIEQLYTNKKAPVKILDIAAGMGNLLLTVLLNLSNAGYQTEGIGVDIDD
                     TLLAVAASTSDLTQANVQYFHQDGLQELLIDPVDFAISDLPIGYYPNDEKAKEFLTST
                     EEGHSYAHHLLLEQSMKYVKPDGFGLFLMPSGFLETDQSEEIKKWFKEEGYLQGMIQL
                     PDELFRNKQSQKSILILQKKGPQAQQVKEVLLVKLASLKEPEKVTEFFNEFKNWKSSA
                     L"
     gene            12637..13821
                     /locus_tag="U2K71_000880"
     CDS             12637..13821
                     /locus_tag="U2K71_000880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate kinase"
                     /protein_id="HEM7314986.1"
                     /translation="MSKTIAINAGSSSLKWQLYQMPEETVVAKGIVERIGLQDSIFTI
                     KYGDNQKFEQIMDIENHEVAVKMLLDQLIELQILSSYDEITGVGHRVVHGGETYGDSV
                     VIDEKVMDRIAELAEFAPLHNPANLMGIKAFKKILPDILSVAVFDTSFHSTMPKQNYL
                     YSLPVSYYEDFKVRKYGFHGTSHRYVSERAAKLLNKPIEELKIITCHLGNGVSITAVD
                     GGKSMDTSMGFTPLAGVTMGTRSGDIDPAVLPYLMEKLEIDIDEMINVLNKKSGLLGL
                     TGLSSDMRDLEKNYEKEHIRLAYDIFVDRIRKYIGGYVTTMNGVDAIVFTAGIGENDG
                     HVRSEIIKGMSWFGCEIDPALNELRGEEVDISTKESKVRVLVIPTDEELMIARDVERL
                     RG"
     gene            complement(14258..15841)
                     /locus_tag="U2K71_000881"
     CDS             complement(14258..15841)
                     /locus_tag="U2K71_000881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM7314987.1"
                     /translation="MIIQAISIKKTIGGNPLFEDLSCQIEAGEKIALVGINGTGKSTF
                     LQILTGREGVDSGVISRKKGLKIGTVEQELTVSEATVNHYLLHSAAEIQDLKQQMSRY
                     EALMTEPDTNLEKCLARYGELQHRFEELGGYVLEDRIAAILQGLGIPHRQEARLTELS
                     GGERVKVALAKILAADADLLLLDEPTNHLDLNSIRWLENYLQNTKKSVLVVSHDRQFL
                     DQVTTKTWELEEGALIEYPGNYSRFRVLKEARLAELTKNYELQQKEVQRLKVMIRRFR
                     QWAHEGDNESFFKKAKELERRLAKLTLVKPPPPPKNRLQSLSNGGKSGKEVFIIQNLH
                     QQYADQVLFKDSSFAVYRGDHLAIIGDNGAGKSTLLKLLLGEEKPYGGTIKLGSSLQI
                     GYLPQQLQFADPDSRLLAYAITLTGNEEVARRQLAKSGFYQSDVGKRIKDLSGGEKIR
                     LALLKLFLEKINVLILDEPTNHLDSYAREEIEEMLQDYQGTLLAVSHDRYFLQQHFQE
                     ALVIDQEQISRQPLGQLID"
     gene            complement(16419..16562)
                     /locus_tag="U2K71_000882"
     CDS             complement(16419..16562)
                     /locus_tag="U2K71_000882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="portal protein"
                     /protein_id="HEM7314988.1"
                     /translation="MEVSDESDTKKYEVSMADTDFIAKLKFYFGFGYREKASATFGKS
                     FKN"
     gene            complement(16785..17264)
                     /locus_tag="U2K71_000883"
     CDS             complement(16785..17264)
                     /locus_tag="U2K71_000883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM7314989.1"
                     /translation="MLQQYKKIMVAVDGSDEAELAFKKAVNVAIRNNGELLLAHVIDT
                     RSFQTVSSFDGMLAEQATEMAKQTLADYESNAKKAGLNNVTSVVEYGSPKQIIAREIP
                     EDNQVDLIMLGATGLNAVERLFIGSVSEYVIRNAACDVLVVRTDLENQLPAKDHTKA"
     gene            complement(17337..17621)
                     /locus_tag="U2K71_000884"
     CDS             complement(17337..17621)
                     /locus_tag="U2K71_000884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7314990.1"
                     /translation="MIRILMIIATLVLLFVSYYLFNKQDIFFVLINKNDKNQGFLQFY
                     GAAYAVLGVMGILTAFFNQRFIALVFLLIVIIVSATFSIRFAKKIAEPKQ"
     gene            complement(17699..17893)
                     /gene="ssrS"
                     /locus_tag="U2K71_000885"
     ncRNA           complement(17699..17893)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="U2K71_000885"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(17965..19251)
                     /locus_tag="U2K71_000886"
     CDS             complement(17965..19251)
                     /locus_tag="U2K71_000886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication-associated recombination protein A"
                     /protein_id="HEM7314991.1"
                     /translation="MQQPLAYRMRPRTIDEVVGQQHLVGEGKIIRRMVDARMLSSMIL
                     YGPPGTGKTSIASAIAGSTNYAFRMLNAATDSKKDLQVVAEEAKMSGTVILLLDEVHR
                     LDKTKQDFLLPHLESGRIILIGATTENPYITINPAIRSRTQIFEVKPLTEQDIQLAVE
                     HALRDKERGLGQQAIQLDEDALLHLSRATNGDLRSALNGLELATLSTPSDKEGRIHLT
                     LSIIEECVQRKALTHDKNGDAHYDVISAFQKSIRGSDVDAALHYLARLVEAGDLASIC
                     RRLMVIGYEDIGLGNPAAAARTVNAVLAAEKLGLPEARIPLADVVVDLCLSPKSNSAY
                     MALDAALADIREGKAGDVPDHLRDSHYKGAKSLNRGVGYQYPHHFDQAWVNQQYLPDK
                     LKNAQYYQPKDTGKYEQALGQQYYRIKEWKKHPPKN"
     gene            19389..20060
                     /locus_tag="U2K71_000887"
     CDS             19389..20060
                     /locus_tag="U2K71_000887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HEM7314992.1"
                     /translation="MTDKQKIDEFFWNKTTPEVARDLLGMYLEHDTPEGRLAGYIVDA
                     EAYLGPEDEAAHSYGLRRTPRVRAMYEKPGTIYLYTMHTHRILNIITQPEGIPQGVMI
                     RAIEPAAMIDQMSKNRGGKTGPDISNGPGKLVEALAIPQELYGQSIADSSLRLVFEKK
                     KTPKKINALPRIGIPNKGVWTEKPLRFVVSGNPYISLQRKNQIEKDWGWRKENEKEGS
                     INIFR"
     gene            20026..20514
                     /locus_tag="U2K71_000888"
     CDS             20026..20514
                     /locus_tag="U2K71_000888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3013 family protein"
                     /protein_id="HEM7314993.1"
                     /translation="MKKKDLLTYLDEIIEKQVTDYDVALDWDTKNHTIEIVVRLFAEN
                     KAQFEIDDAEGIVSQEPIIEFEDGVLLFNPQKSVFDERNYLAVIPYEGKKGLAKSVAD
                     SLVEYLNEVLAQGQSDLLDFLDEDDDEAVFELHWDNQQLAELVEVKQQNEADTYLPYP
                     SY"
     gene            20527..21474
                     /gene="prmA"
                     /locus_tag="U2K71_000889"
     CDS             20527..21474
                     /gene="prmA"
                     /locus_tag="U2K71_000889"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HEM7314994.1"
                     /translation="MKWNEVKVETESEAVEAVANILMEAGASGVAIEDSLDVEHFQSD
                     QYGEILDKNDFTSLKEGALVMAYFPETTFLPEILPFIKDRITQLPEFGLAIGKNKVTV
                     SEVEESNWATAWKKYYHPVRITRMLTVAPSWENYQTEDPLEKIITLDPGMAFGTGTHP
                     TTSLTLTALETVLRGGETLLDVGTGSGVLSIAAKYLGAKDVYAFDLDEVAVRSAKENM
                     DMNEIAKDVHVSANDLLTGIEIESDVIVANILADIILLMIPDAWRLLKQTGTLIVSGI
                     IEEKKQMVLDAMLEQGFIVDQIFQQKDWYAIILKKPEVE"
     gene            21475..22239
                     /locus_tag="U2K71_000890"
     CDS             21475..22239
                     /locus_tag="U2K71_000890"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HEM7314995.1"
                     /translation="MQRYFLKESYESKDYFVLEGENYHHIVRVMRMEPEDEVFLAFND
                     QIAIVAEITEITEEAVYLKEIKKEENQKELPSSITIASGYPKGEKLDWIVQKSTELGA
                     QAFLGFPASASVVKWDAKKRMKRQQRLEKIAQEAAEQSHRQIVPSVELLETKQDLFER
                     LDDYDVVLVAYEESAKQGEKSNAAKILSETKPDSRILAVFGPEGGLSRDEVDYFVEHK
                     GILCGLGPRILRTETAPLYLLSAASYQWELMNPSFS"
     gene            22472..24685
                     /locus_tag="U2K71_000891"
     CDS             22472..24685
                     /locus_tag="U2K71_000891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HEM7314996.1"
                     /translation="MTKEEILTGPGVIKLVSQYMGPEHVAFVEKACEYATAAHDGQFR
                     KSGEPYIIHPIQVAGILADLKMDPHTVATGFLHDVVEDTEITLEDLREEFGDDVAMLV
                     DGVTKLGKIKYKSHEEQLAENHRKMLLAMAQDLRVIMVKLADRLHNMRTLKHLREDKQ
                     RRIAQETLEIYAPLAHRLGISRIKWELEDTALRYLNPKQYYRIVHLMQTKREEREKYV
                     SGTVEDIRIATEELGIFAEIYGRPKHIYSIYRKMKDQKKQFNEIYDLLAIRVIVDSIK
                     DCYAVLGAIHTKWKPMPGRFKDYIAMPKANMYQSLHTTVIGPAGNPVEIQIRTQEMHE
                     IAEFGVAAHWAYKEGKNEKVEPDGMTKQLSWFHEILELQDESYDASEFMEGVKGDIFS
                     DKVYVFTPKGDVTELPKGSGPLDFAYSIHTDIGNKTTGAKVNGKMVQLDYKLKNGDII
                     EIMTSPNSFGPSRDWLKLVATSKARNKIKRFFKAQDREENVIKGHESVVKCITDLGFT
                     PKDILTKNKLQEALDRFNYQTEDDLYAAVGYGEVSPLTMANRLTEKERKEQKIEQQKQ
                     EAEEIMNQPKKEPDKMKVRHEGGVVIQGVENLLIRISRCCNPIPGDDIVGYITKGRGI
                     SIHRRDCPNVQPDKPNVAERLIEVEWEDTSNTRKEYDADLEIYGYNRSGLLNDVLQTV
                     NALTKNLNSVEARTNKDKMATIHLTVGIQNLSHLKSIVDKIKAVPDVYSVRRTNG"
     gene            24696..25142
                     /locus_tag="U2K71_000892"
     CDS             24696..25142
                     /locus_tag="U2K71_000892"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-tyrosyl-tRNA(Tyr) deacylase"
                     /protein_id="HEM7314997.1"
                     /translation="MRAVVQRVSKAAVSVDQQEIGKIGYGLLVLLGIHETDTQKDVDY
                     LVKKIAQMRIFEDEQGKMNISVEDVGGQILSISQFTLFADTKKGNRPSFVEAARPETA
                     IPLYESFNEGIRSRGITVATGQFGADMSVSLVNDGPVTIIIDSKNK"
     gene            25313..26572
                     /locus_tag="U2K71_000893"
     CDS             25313..26572
                     /locus_tag="U2K71_000893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HEM7314998.1"
                     /translation="MKEVPVVDIKITDLKKLYQKGYNMLEELRHEADAPVVKAKIFNK
                     EAITIYGSSAAKVFYDPRNFKRKGAMPKLVLKTLFGQGGVQTLDGAAHHHRKNIFMDL
                     MTPERMEDYHRILDKNLTQALEAQHGQFELFDLSKMVFFTSICEWAGINLSAISKDEV
                     EKLAEYQISMISGTFTSPIDHIKGVENRKKSEKWAQGLIEEARQNPVAGKENVALYAF
                     ANATDLDGQLLPLEVAAVELLNIIRPTVALTVWAALMGHALFSRPDLYQQLKNDFSTL
                     QDPFIQEMRRYYPFFPMLPAISLKEVEVDGYRIPEGSWVILDLYGTDHDERTVEAPDS
                     FMIKRYVGKAKDISYKEEYEMIAQGGGNFRQMHRCAGEWITLHSLRVFSDQLVNKFEF
                     SVPEQDWTIPFNQFPTYPNSRALLYKN"
     gene            complement(26661..27608)
                     /locus_tag="U2K71_000894"
     CDS             complement(26661..27608)
                     /locus_tag="U2K71_000894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEM7314999.1"
                     /translation="MAEIRIGHSQVYAEQLGLGANAVGGHNLFDGLEDETGKQVVRTA
                     LNSGINLIDTAYAYGNGRSEELIGEVLKEKEYDRSRVVIATKAAHVPNKGRTFDNSPE
                     FLKQSVEDALKRLQTDYIDIFYIHFPDESTPKNESVATLHELKEAGKIRAVGVSNFTL
                     EQLKEANADGYVDVVEDKYSLIHRQAEKELFPYLEKNKISFVPYFPLASGLLTGKYEL
                     GEEKQFGEGDPRKRNPDFQGERFREILTAVDVLRPIAKRYQATPAQLVLAWYMKNPRV
                     SVVIPGAKRPEQVSDNVQALDLHLSNEDYQTIDEAFRGF"
     gene            28002..29294
                     /locus_tag="U2K71_000895"
     CDS             28002..29294
                     /locus_tag="U2K71_000895"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HEM7315000.1"
                     /translation="MSFQRPKGTNDILPGESEKWQFVEETARLLFRDYQYNEMRTPIF
                     EHYEVISRSVGDTTDIVSKEMYDFYDKGERHVTLRPEGTAPIVRSFVEHKLFGPEYAK
                     PFKTFYMGPMFRYERPQKGRLRQFHQIGVEAFGSENPATDVESMVMALDFFKQLGITQ
                     IRLVINSLGDKETRQAYRQALIDYLLPFESELSEDSKRRLHENPLRVLDSKDKRDQKF
                     VAEAPSILDYLSEPAQKHFDTVVSMLDALAVPYEIDSNMVRGLDYYTHTIFEIMSDAP
                     KMGAQATICAGGRYDNLVEELGGPSTPGFGFAMGIERLLLTMEAEEVVIPAFNELDAY
                     VVALGDETNIEALKVVQAIRNFGFSADRDFMNRKAKAQFKTADKANAKLVLVIGEDEL
                     ASQTINVKAMASRKEKSFPLEEIYEHFDEVYDEMTLFE"
     gene            29309..31078
                     /gene="aspS"
                     /locus_tag="U2K71_000896"
     CDS             29309..31078
                     /gene="aspS"
                     /locus_tag="U2K71_000896"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HEM7315001.1"
                     /translation="MAQRTIYCGLVSEQQLEQTVTLKGWVQKRRDLGGVIFIDLRDRE
                     GIVQIVFNPEINKEAWEIADKCRSEYVIEVTGEVRRRGENAVNPKMATGEFEVIASEI
                     VILNQAKTPPFQIEDDQAISDEIRLKYRYLDLRRPKMTKNIKLRNATTKAIRHYLDDN
                     DFLDIETPYLGKSTPEGARDYLVPSRVHAGHFYALPQSPQLFKQLLMNAGFDRYYQIV
                     RCFRDEDLRGDRQPEFTQVDIETTFLTAEEIQTYTEGLIAKVMKEVRGIEVTLPFPRM
                     TYDEAMARYGSDKPDTRFDMELIDLSDTVKEVEFKVFQMALENGGVVKALNAKGAADR
                     YSRKDMDQLGQYVGQFGAKGLAWLKVEEDGLKGPIAKFMGEATEAIIKATDAKPGDLL
                     MFGADKSEIVAAALGAIRTRLGKELGLIDESKFNFLWVTDWPQFEFSEEEGRYVSAHH
                     PFTMPKEEDIPLLAEDPAKVYAEAYDIVLNGYELGGGSIRIHTRELQEKVLETLGFTK
                     EQMEEQFGFLLTALDYGFPPHGGIALGLDRFAMLLAGEDNIREVIAFPKNGKAADVMS
                     DAPSMVSPLQLFELNIDVTNIEE"
     gene            31187..32062
                     /locus_tag="U2K71_000897"
     CDS             31187..32062
                     /locus_tag="U2K71_000897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM7315002.1"
                     /translation="MKKTFENWAYHDYTTKLSVSIVYALIASVALNFFYQPGHIYSSG
                     ITGLAQILTTLSKSLIGFEVPVSTTLYLLNIPLFVLAWLKIGKKFTIFTFITVTLTSL
                     CIHILPETVLSTDPIICALFGGAVMGLGIGLALRNGLSSGGLDIVSITIRKKTGKSIG
                     SISIYFNALIVFVAGILFGWQYMFYSTLSIFVSGKVTDVVYTKQKKMQVMIVTKHPEK
                     VVNEIQNCMRRGITIINEAEGAYKHDKQTVLMTIVTRYELPLLREAMKASDPQAFVSI
                     AENVQILGRFYEDGI"
     gene            32328..34211
                     /locus_tag="U2K71_000898"
     CDS             32328..34211
                     /locus_tag="U2K71_000898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEM7315003.1"
                     /translation="MEKQKRLKNSRYITGFDGLRSLAVVGVIFYHLLPTSMKGGYLGV
                     PIFFVVSGYLITDLLRQEWEQNGKINIWQFYVRRMKRLYPALVFMLVTASAYITLFQR
                     GLLNNLRGVVVSSLLYVNNWWQINNGLSYFDRFANESPFTHIWSLAVEGQNYLVWPIL
                     FVLLMVFVRKRKWIFYTVLGTSFLSAILMMILYTPGGDPTRVYYGTDTRLFSIWLGSA
                     LAFVWPSTRLKKNIPKQAKNILNIAGGISLLALILCFFFLDDHYNFIYYGGMLLVSLL
                     CVVLVAVTAHPGASLNRWLTNPVFTWIGKRSYGIYLYQFPVMIFYEAKVTDIADHVLL
                     HTLVEITLILIISELSYRYVERPLARFDYGRTFQVMKGWFSKPIISKSKPWVVPGTLL
                     VAIALVGFVTAPKNAVTADQKQLQAKIAESKKLAEETRNNSENTTSSVDQAILDKYEL
                     SKAQGNKAQKLELTAFGDSVMLDAATDLQELYPKVVVDGDVGRQLYASVPYIEKLKEQ
                     DLLKDTVLIGLGTNGSFSEAQFDSVMDAIGDRKVYWVNVRVPTKRWQNDVNAMLEQMT
                     KKYDNMTLIDWYSYSNDHSDWFYDDQVHPNPEGMTHYIHLVSEALLGGSSSTNSSETN
                     VTE"
     gene            complement(34286..34570)
                     /locus_tag="U2K71_000899"
     CDS             complement(34286..34570)
                     /locus_tag="U2K71_000899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEM7315004.1"
                     /translation="MANEHYFYVLFCKDQTFYGGYTTDLSRRLEEHNNGTGAKYTRLQ
                     KRRPVKMIHAEVFSSRSEATKAEAAFKKLTRKQKERYLQTHSTVLLPESI"
     gene            complement(34560..35294)
                     /locus_tag="U2K71_000900"
     CDS             complement(34560..35294)
                     /locus_tag="U2K71_000900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA1(Val) (adenine(37)-N6)-methyltransferase"
                     /protein_id="HEM7315005.1"
                     /translation="MLHEDERIDQLYAEDIKIIQSSKVFSFSLDAVLLAHFSRVPKKG
                     KIVDLCAGNGAVGLFLSKRTQAAIDAIELQPRLADMARRSIALNHLNEQMTVHTIDLK
                     DSLSVVRHNSCDLVVCNPPYFKGLPTNKTNPNKHLAIARHEIHTTLEEVIDISSKLLK
                     TNGRFTMVHRPDRFLEIIQVMEDYRIAPKRIQFVYPKPGKEANILLVEGIKEGKLDGF
                     KVAPPLITYNMDGEYTAEIREMLYGK"
     gene            35423..36046
                     /locus_tag="U2K71_000901"
     CDS             35423..36046
                     /locus_tag="U2K71_000901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-acyl-sn-glycerol-3-phosphate acyltransferase"
                     /protein_id="HEM7315006.1"
                     /translation="MFYRFMRGVVRVLLALVNGNARYENKEVLPQDENYILVAPHRTW
                     WDPLYLAVAAAPKEFSFMAKEELFKNPVLRFILTRSNAFPVKRENPGPSVIKTPVKIL
                     KSTNLSLIMFPSGTRHATELKGGMALISRMSKAKIVPAVYQGPLTLGDLFKRQRVTVR
                     FGEPIDLSDIKKTDKEGMAEIERRTQAAFDKLDKEVNPDFKYEVKKK"
     gene            36238..37359
                     /locus_tag="U2K71_000902"
     CDS             36238..37359
                     /locus_tag="U2K71_000902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HEM7315007.1"
                     /translation="MRFLHTADWHIGKKLQGYDLIEDQRHVLSSILDIAKEEKVEAIV
                     IAGDLYDRSVPAVDAVELYNELMVDWNLKEKIPIFAISGNHDSSTRLSAGTPWFSYSN
                     YFLRTQLAEAFEPVEFKNVQFFFLPYFEPIAARLYFEDDNIRTIKQAVAKVIDKMQEL
                     FDSEKKQVLISHFFVMGSVKSDSETKIEVGGLDSIPGELLTPFDYVALGHLHNQAALR
                     QDNARYSGSPLKFSLSEINQKKGVWIVDLTTGLELNFREIEPLHDIEEITASFKELLD
                     PAFYQSVNRENYIYIYLTDRTIIPNMMNQLRQIYPRILGIERANGRAEQTKRKRKVLE
                     MKDPQKLAEAFFSEMTEEQLTANQEKWLGETLQEIMEME"
     gene            37366..40494
                     /locus_tag="U2K71_000903"
     CDS             37366..40494
                     /locus_tag="U2K71_000903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HEM7315008.1"
                     /translation="MKPKKLQLKNFGPFINETVDFSRLTEAPLFLISGKTGAGKTTIF
                     DGITFALFGETSGRLRSGKEMRSLFATPDEETSVTFSFEHQQMSYEIERKPEQVLAKR
                     KGNGTKKQAAKVTLTIFDGKGKEIKQITKRTEADQLIRELMNLDAKQFSQIVLLPQGE
                     FRNFLVSSSSDKETVLRHLFGTQFFQQFNERLKEKAKQQQQKLDHLEQELHLLQKRFV
                     LVKEEEPAAGFEAVLAQWTNHQAILEKQITEEKEALTELQEKQKQLENSYYRFESLKK
                     SYEEKTILINKQKELAAQKDEIEEKKRWIGYYEFTQRLVEPIQRLQEIKVEKIELSQK
                     SSEQTKKLAMTTQNYEEWQKNEANRVRRQTKIKEAAQKLQNKQMMVPIVESLRSKKQD
                     AEQLTISLDKEELNAEKLYQNLAIYQERTRILQENLLDQEKLQEDRLAFGQLKYLDEK
                     QQEIEKQKAKDTGSLAQIREELKKATEEITKNSQLLKEQTEQAEQLKSQWAKQQIARL
                     QLFLRPGEPCLVCGSTEHPIQQLKHEQPNKEDILKVEQQLAEAESAVERTQQIIAKSE
                     AHENNLQQQLSDLEQKIMATNIQLAEQEEHLQQALKKAFPEITEQKTKTTLVVIEQSL
                     DRRKSKMANARIELEEIRQQAEKLEQQLAVYKQQQTEKNNQLQQLQGEIKSLEQQLQD
                     TDSNCLEEEIRRLEKQLEEDSNQLAVEQKAGVQLLQDLHTLRTQLDLQHEQIEKIDEK
                     IEKEQERIAKKLESQSYFSTIEEICAFAQQQAQYKENQRTVTSYHEEQLVTADRLRQL
                     ENIQLTETFPDTEEISQQLEEIRRMIEIKQEKIYGLQEQKISNQKIYDECLSIYHTSQ
                     TKLDELTQLQQLSQTFNGENPKKTSLERYVLQVYLQEVLQVANDHLQRLTKNRYQFEL
                     ADTIGSYRGKTGLEINIYDDQAGMSRSAHTLSGGESFIAALSLALALADVIQTQAGGV
                     AIDALFIDEGFGSLDEEALEMAMEALETIESEGRMIGIISHVRELKERVLQQIRIDTE
                     GSGQSKIRYQLG"
     gene            40513..43707
                     /locus_tag="U2K71_000904"
     CDS             40513..43707
                     /locus_tag="U2K71_000904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SNF2 helicase associated domain-containing
                     protein"
                     /protein_id="HEM7315009.1"
                     /translation="MKWSIPEKVIARGRQYLQEGRVLSVVPDQEENVWHAEVLGSELY
                     MVDLDGTAKEIDYCQCPYWEEHKYCKHTVAVELYLRSKNLSRIMKKDQPMLEPVKATS
                     EGEIIAKGFSRLKTQNNPQAVQPLIIEYQVETIETNQYHPELSILALYLRIGSLASPK
                     KTYIVKNIYDFLQAYTEKETYTVNKQYQFRLDKRAFQKEDQKILTHLAGSAQTHQLLG
                     TNGLQVKGKLDKRYLLLPVEQARFLLEQMNQTTRLHLQINEQNDQGIQFSDQNKPLSF
                     KVEKFGEDYLLQALNDFDFFFMHYQWGVIQGTFYSLTKYQQTVYQTWKQLIRRLEKPE
                     VVFKKKELPSLFKEIIPLLSEIGDVAIAPAVEEEVADIPVEFIFFFRKAKGKIQARVD
                     FVYGEVIYSTDPKHEVSTEQAFQVLRDTVEEKRVLDLFRMYRYQKNETGYERPLPSGE
                     ELYAFFRTELSVFRQFGEVRLGRKLRELYLDAHKHQPKIEVTETGSWLDIRFDVTGIE
                     EQEIDHVLQSLLRNDAFYTLENGQVLSFDSEEFQQTSQVLQQLRESIRTEEGTIHVPK
                     NQGLIIQNQLEKSNATFSESFQTMVQDLIHPERYQAQLPKGLNATMRDYQKQGFRWLK
                     MLGHYQFGGILADEMGLGKTLQTIAFLLSEKEERKSFSALIVAPASLIYNWQAEVRKF
                     APSLSIQVINGNKKEREELLAKDTDIRVTSYASLRQDLANYQSQKIDYLILDEAQMVK
                     NSSTKTAQALRELAVPQRFALSGTPIENNLEELWSLFATIMPGFFPTKTKFRELAAEE
                     IAQMIRPFILRRDKQTVLKDLPEKTEMNLYSALTEEQKTVYLAYLRQMREEITSMDSE
                     AFKKNRIGILAGLTRLRQICCDPRLFIEDYQGGSGKLEQVKDLLVAAKENKRRVLLFS
                     QFTGMLTILQEELAELGISTFYLRGSTKPQDRLSMVDAFNAGEKDVFLISLKAGGTGL
                     NLTGADTVILYDLWWNPAIEEQAAGRAHRIGQKNVVEVWRMISEGTIEERMDSLQQEK
                     RELFQKVIQGNEEQLTKLTEEDIRMILSVGEIEE"
     gene            complement(43853..44560)
                     /locus_tag="U2K71_000905"
     CDS             complement(43853..44560)
                     /locus_tag="U2K71_000905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM7315010.1"
                     /translation="MIKSVVFDVDDTMYDQQQPFRNAVKRVVPLVSDADMHPLYIRFR
                     HHSDENFPKVMAGDWTLEYMRAHRISQSLKDLDYPHITEEDGLLFQKIYEEELDNICL
                     HEEVKKTLDFLKEKNVPLGIITNGPTDHQTKKLKQLQLNNWIPSRNMLISQATGFQKP
                     EKEIFQLAEKEFHMLPEETLYVGDNYDNDVLGAKSANWQALWFNHRERKIEQTPICDI
                     EISSFDQLLETMKTIFV"
     gene            45325..45723
                     /gene="spxA"
                     /locus_tag="U2K71_000906"
     CDS             45325..45723
                     /gene="spxA"
                     /locus_tag="U2K71_000906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEM7315011.1"
                     /translation="MLTLYTSPSCTSCRKARAWLQEHQIPFVERNIFSEPLNSSELKA
                     ILQMTEDGTEEIISTRSKVFQKLNMDLDDLPLQELLELVQNNPGLLRRPIMIDDKRLQ
                     VGFNEDEIRRFLPRDVRQLELRQAQLMAGL"
     gene            46055..46708
                     /locus_tag="U2K71_000907"
     CDS             46055..46708
                     /locus_tag="U2K71_000907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adaptor protein MecA"
                     /protein_id="HEM7315012.1"
                     /translation="MEMEHINENTIRVLIGHEDLEERGVSFLDLLGNHKEIENFFYSI
                     LEEVDIEEEFRGSEAVTFQVLPKGDGLELFISKNLPDEEISQSEDSTVDSEDVSDFLR
                     QQIIGSDPEEELSGSALEEQERQATFAFEDFEQVIQLATDVELESAWTDLYLFEERYY
                     LVVHFWMENLNQADVENQIARILEFAEKSDRTPETLNEYGQCLMARNAIERTKFYFV"
     gene            46934..47932
                     /locus_tag="U2K71_000908"
     CDS             46934..47932
                     /locus_tag="U2K71_000908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="competence protein CoiA"
                     /protein_id="HEM7315013.1"
                     /translation="MLLAMNEYNKCCFAKHAEKGKKYYCRGCRKRVILKKGKKKCAHF
                     AHQKSDNCSVFSERESKEHLQLKECFMDWLGQSVEPVFLEAYLPRLRQRPDILLANLA
                     IEIQCSRLSHQRFIERTQNYLNNSYQVWWILGNSFLGQSQFSLIEKSCCYYNRKRGVH
                     CWKADLKKQKLYLYHHITETVSGHISFCSSCWTFSSRDLKEIFTSNEIKMNQMKKIEK
                     VSEDGKKWLTRQLIHKQKNTVSIQEQCYLRHKHLLHLSSWIYQKSRFFFYLQEQVFLY
                     RMLYEEALENQKVPDFNSWLCQIKEHKREWLFPMIDEEMVYQQFFNECIHLSSLKK"
     gene            48108..49916
                     /gene="pepF"
                     /locus_tag="U2K71_000909"
     CDS             48108..49916
                     /gene="pepF"
                     /locus_tag="U2K71_000909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligoendopeptidase F"
                     /protein_id="HEM7315014.1"
                     /translation="MEVKQLPKREELPENLTWDLTKIFSSDQEFDEKYLELSEELKQS
                     EKHKGTLDQGASQFLNAIEFVLRVYRQTEVIYVYAHLKNDQDTGNTDYQALYARASSL
                     FSKVSEAVSWFEPEILQLSDDQIWQYFKEEPKLEVYRHYIQQIVDNRAHVLSAEQESL
                     LAGAGEIFDASSDTFAVLNNADLVFPTIEGENGEIVQLSHGVYGQLLESTDRRVREAA
                     FKGLYSVYEQFRNTFASTLGTHIKGHNFKAKVRNYSSAREASLSNNHIPESVYDTLVD
                     VVNKHLPLLHRYMELRKRLLEVEKLHMYDLYTPVLGEAPITFTYEEAKEKALEALKPM
                     GEEYMAIVEKAFSERWIDVVENKGKRSGAYSSGSYDTNPYILLNWHDTLDQLFTLVHE
                     MGHSVHSYFTRSNQPYVYGDYSIFLAEIASTTNENILTEYLLETEKDPRVRAYVLNHY
                     LDGFKGTVFRQTQFAEFEHFMHTEDEKGVPLTSEYLSDSYGKLNAKYYGPAVEEDPEI
                     KFEWSRIPHFYYNYYVFQYSTGFSAASALAKKILNQEPEALENYLAYLKAGNSDYPVE
                     VMKKAGVDMTQAAYIEDAMSMFEQRLNELEELIDRL"
BASE COUNT        16175 a         8741 c        10454 g        14626 t
ORIGIN      
        1 ttttgatgaa tcagttgttt cttcagttga agattctttt gttgaatcag ttgattttgt
       61 gtcagaactc tttgttgaag ctgtagtttc acttgagtct ttcgtacttg atgaagttgt
      121 tgtgaatgca ctcaacacat tttcaaaggc atcatctttg atttttacat ttgcatcttt
      181 taattcttca ccgattactt tagttgtgaa tgcattgtca cttagtttag tttcagtagc
      241 gatatctttt agttgatctt tgtatttatc catgtcattg cctttatttt ggtttttcac
      301 cattttgaca acgtagtatt ctgtcgcata agaagtagga tttgttgtag taataacatc
      361 ggagatctca ccgtctttca atttgaaagc tgcttctttt acttctgcag gaatagttgt
      421 tgtcgttgag tcgaatttaa ctttaccgcc atcttctttt gtctctgtat cagttgattt
      481 gtctttggct aatttagaga aatcatctcc atcatccgct gattttttca catcttttgc
      541 ttcatcttca cttgataatt tgatgatttg tgcttctact tctggatgga aagatttcca
      601 agcagttttc aaatcgtcat ctgtaatatc tacgtgagct ttcaagccag cttcaaaagc
      661 taaattgtta cgaatatatt ctttataagt atctttcgta tatccagcag cttctaattg
      721 tgattcaaaa gtatcaccta atgatttcgc ttgtttgtcg tattctgcat ccacttgctt
      781 atcggttact ttatcgccat atttgttatt gaatacttta taaatgatca tccgttgtac
      841 taaggattgg ttcgatgatt caagttttgc ttcatcatag aaatcagata cagtgatcgt
      901 tccgcctttc atcgttgcga tatctttatt agtatcgcct gaacaagctg ctagagttaa
      961 aacagccaac gcactagtag ctgccaaaat aattgatttt tttttcatta aaatttgcac
     1021 tcctgtaatt agttattatt tttatatatt aactatacca tatcccattt cctttaaaaa
     1081 cgttgtcgtg gtagtttcaa acaaaattca aaatttcatc acattcctag gaaatacgca
     1141 aacttttctc acttatatag ttaaatctgt tgagaaaata atcattatta tatttgttca
     1201 ggatactctg gcagatcagc ttttagtttt tctacttgtt cattgatttg tgtgatacgt
     1261 ggttcggctt ggaatttaaa atcttcgata tcttttttga ttgcttcttg gaaagcagga
     1321 atcacagatt cagctgtttc ttttgtttcg atcaatgcgc ttttaaaatg attcaggctg
     1381 ttatttaaat catttgtcag agttgttgct tcttccaatt cttcgatcag tttctttctt
     1441 gtttcattcc cgctcctggg cgccaataac agacctccga cagtgccagc agcagctcca
     1501 aataacagtc ctttgaaaaa gtttttaatc atcgcaggac acctgcttta cgattgtttc
     1561 ttgaatagct tttagttctt ccgatccata actatcttga tgattgacaa agtgaataga
     1621 aaaatcatcc tctttgctgt agcgagggac taaatggata tgtgaatgaa aaacagattg
     1681 ataggctagt tcacggttgt tattcaagat attaagacct ttcatttctg gaaaagcttt
     1741 ttccaatgct tgagcaatct taggcacacg cgaaaaaacc tctcctgcaa gttcgggatc
     1801 atattcaaaa atatctgcta catgacgttt aggcacaact aatgtgtgtc ctttcgttgc
     1861 ttgagagatg tctaaaaaag catagacctt gtcgtcttca tatattttat agcttgggat
     1921 ttcttgattg atgattttgc aaaaaataca gttttccata taaaacatcc tttctataaa
     1981 gaataaatac ttcagcttaa ctttaccata agttaaagct attgtaagga aaaaaacaaa
     2041 ataccgtcat tttcttcgtg gtataataaa taaaaataaa tggcggagga aaaagaatga
     2101 gtttaattat cgatcatgtc acaggggggt acggacatct ccctgtgtta aaagatatca
     2161 gtttcgatgt acagcctgga gagatagtag ggttgattgg actgaacgga gcagggaaaa
     2221 gtacgaccat caagcacatt atcggtctgc tggaagcttc aaaaggaacg atagaaatca
     2281 atcatctatc cttaaagaaa aatacggaag aatatcgcaa acaaattggg tatattccag
     2341 agactccttc tttatatgaa gaattgacat tgagagaaca tatcgaagtc actgcgatgg
     2401 cctacgatat acctatggat atagcaatga aaagagcaga aaagttgttg gagacttttc
     2461 ggttatctaa tcgtcttgac tggttcccag ctaatttttc aaagggaatg aaacagaaag
     2521 tgatgatctt atgcgccttt ttgatcgagc cttctttata tatcatcgac gaaccatttt
     2581 taggcttgga tccactagct atcaatgcat tgcttgaatt gatggtagaa atgaaaaaag
     2641 aaggagccgg tattttgatg tctacgcata ttttagctac tgcagaaaaa tattgtgatc
     2701 gttttgtggt cttacataac ggagagatac gagcagacgg ttctcttgag atgttgagaa
     2761 aagaatttca gctgccggat tcttctttgg acgaaatcta tattgcttta acaaaggagc
     2821 aggcacatga gtagtttttt tcaaaaacgt ttagcaagac atcaaaagaa aatgatgaaa
     2881 tatatgcgtt atgtattgaa cgatcatttt gttttagttt gtcttttctt gattggcggt
     2941 gtgggattat attattctaa ttggttgaaa accctgcagc caccgtttgc aattggaggg
     3001 ctcataatct ctgtattttg gatgctctgt ttattcgtgg gtaagatggc tacttttgca
     3061 gaggcagcag atattgtttt tttacttcct aaagaaaaag aaatgcaaac ctatttggaa
     3121 cgagcttttc gctattcttg catttttccg ttgatctttt tgatattggc atgcggattg
     3181 gccatgccat tagtggtggt tagtacggag caatcctttt cgatgttttt cgtctatctg
     3241 atcatattat ggagtttgaa attcagtcat ctgcaaatca agagagcaga attgtttcga
     3301 ctagatcaac aagttattcg ttattggaaa ttaggctggt ttttttcttc tctgatcttt
     3361 ttgtttctat cattttattg gctaatatgg gcaggaatag caggtgcatt agtacaggca
     3421 agtctatatt actggttttt gtggaagcaa caagaaaaag gattggattg ggagaaaatg
     3481 gtgcatgtag aagaacatag gttgcatcag atctatcaat ttatcaattt attcacagat
     3541 gttcctgaaa ttacggcaaa gatcaaaaga agacgctatt tggatcctat attgactcat
     3601 attaaaagag aatcaaaaaa cacttatctt tacttgtttg caagaagatt ggcaagaggt
     3661 tctgatttta gtggtcttta tttccgatta gtaattatag gtgggttatt gattgccgga
     3721 gtagtcgact tttattttag tctgattatc ggtttcttat ttttatattt gatcggtttc
     3781 caactgttac ctttagcgaa ccagttccgc tatatggttc tcgttcagct ttatccaatc
     3841 cataagaatc agaagaagca ggcgattcag tatctactca catgggtatt aattatcgcg
     3901 tcggttattt ttggtttgat tgctgcagtc gtattagatg ggcagcagcg atgggttccg
     3961 ctagcggctt atttgattgt tactatgctc tttgttaaac tatatgtacc aactcgtttg
     4021 aagaagatgt cagactgaaa acaaagctgt gtagggtaaa aatgagaatg aaaaatagag
     4081 cgttgacagt aagaatgctg ttcgttctat tttttttgag aaaagctggt tttttgaatg
     4141 gcaattaaat gaaaaagaac gtatatgtgc ttgacgtgta gtgttcaggc gactagaata
     4201 ataagtactg caaaacaaag agtaagagga cgggagaatg catgatggag tttcagttag
     4261 accaagagtg gcggttacgt cccatcaaag gcgacacagg aaaaacatac ataggcatgc
     4321 gtgatgagga caaagtcttt attaaacgaa acacgactcc gatgttggct gctctttcaa
     4381 gggaaggaat tacaccaaaa cttgtttgga caaaacgaac ggggaatggc gatactttga
     4441 cagcacaaga gtggctggaa ggtcgcttgc tgactcctaa tgagattggt caaagaaacg
     4501 acgtgattga tgttttgtac catttacatc attcaaattc tcttaaaacc atgctgaccc
     4561 gtatcggtgg tcgtgtcatg acgccggaga agatgctgca aaaatatgaa aaagaattac
     4621 cggaagaact ccagaaaaat caatttctgc aaattgtcca tcgttttctt cgtgagcaga
     4681 tcccgtcaat agataaggaa aacttatcag tagtacacgg agatgtaaac catcgtaatt
     4741 ggttagtatg taaaaattat ttatatctag tcgattggga ttcaatcatg tttgcagacc
     4801 ctgcggtgga tattgcaacg attttaggaa attatgtgcc actgtctagc tggaatcagt
     4861 ggctgatcag ttatggcata cgcccgactg acgaaatgct tgaaaaattt cattggtatg
     4921 cggccatgaa tttgctccaa gaaatcacca gatattgcaa acgtgaagat catcgacgga
     4981 tgaacgaaga aattttacaa ttaaaacgaa ttttcagtgg ctgaggtttg gcccaaactc
     5041 tgcggagatt tggccagacc tttttaatta gaggaaagga atggagataa ataatgcgtg
     5101 tccgaaatcg tccgggagca gcggaaatgt tagcggctca cccgaatttt gtaattagtg
     5161 atccaacatt gtggaaggga aaatggaatg aattgtttga aaacgatcat ccaatccaca
     5221 ttgagattgg tatggggaaa ggccagttca tcactggaat ggcaaaagcc catcctgaaa
     5281 tcaattatat tggagtagaa atgcaggtca gtgttgtttc gattgcacta gacaagttga
     5341 tcgaacaacc gcttcctaac ctgaaacttc tacatgtgga tggatcagcg ttgacagagt
     5401 atttcgctga cagcgaagta gatcaaatct atctgaattt ttccgatcca tggccaaaaa
     5461 aacgtcatga aaaacggcga ttgacatata agacttttct tgctgtagat gaacagattc
     5521 ttcgtccaaa tggagaaatc catttcaaaa cagataatca aggattgttt gagtattctc
     5581 tggcaagttt ttctcaatat ggcatgatcc tcaaacaggt atggttagat ttacatcaaa
     5641 gtcaatttga aggcaatatt atgacggagt atgaagaaaa attttcttca aaaggtcaac
     5701 gtatttatcg agtagaagct caatttcaag ataagaccaa aaagaaaagt ctgtgaaact
     5761 aactttctgc tttcataaaa acattaatat ccataaataa atcccagaaa atgaacatta
     5821 cagatgtaat gtattttctg ggatttattt gatttaaaat gtatttattt catcggtttt
     5881 tccacaaggt ggaatcaatt ggaagcgaga aaatgaaaat aatcctttgt cggtgatctt
     5941 caattcgggt atgacagata atgttaagaa agaaagtgtc aaaaaaggat caaaggtttt
     6001 agctgctcct agttgatgtg ctttagttat taattcattc aactgttcat agacgatcga
     6061 aaaaggttcc tgtgtcatga ttccgcctat aggaagagaa acacaagcta tttcctgctg
     6121 attgttgaca acggtcatcc cgccaccttt ttgaatcaaa agattagcag catacagcat
     6181 atcctcgtcg tttgtcccaa cgacaactag attatgcgag tcatgggcaa tcgttgtcgc
     6241 tatcgctcct tgtttcagct gaaatccttt cactatgcct aatccaatgt ttcccgtttg
     6301 atggtgacgc tcgatcaccg caatctttaa ttggtctttt tcgattgaag gaatgaatcg
     6361 ttcgccttgt cggtccactt tttctaccaa atctttagtc actagactat tcgggataat
     6421 ttcgatcaca tgggcttgtg aagaagaaag aagcagctct aacgaatgtg gatttaatgg
     6481 ttgagcgttt agttttggca gacaacacgt atatgaattt gaatgatcag caaataaatg
     6541 gtcttggagt tgcccagatt gaacaactaa tgttccctta tagaaaactt catgaattgg
     6601 cagcttggta agatcgtctg ttaagaagaa atcagcttga tacccagggg caatcgcacc
     6661 tagtgttttc aaatcaaaac attctgctgc attcaatgtt gccatctgaa tggcttgtaa
     6721 tggatcgatt ccaagtgcaa tagcttgacg gacgatatga tcgattgatc cttcttttac
     6781 taaatcattg atcagtttat catcggtgac aaagaagcat cttcttgaat tttcaggtgt
     6841 gatcgccggt agtagcgatg ccagatcttt tgcaacggtc ccttctcgaa ccattaaata
     6901 cataccaaga tccaaacgtt ctttagcttc ttttgcagtt gtagcttcat gatccgtccg
     6961 aatacctgct gcaggataaa catttaactc ttccatgccg ataccagctg catgcccgtc
     7021 aatttttttc ttatgctggt gcatcaattg gatcttttct aggatgtctg tttcattatt
     7081 tgctactgct tgatagttca tgacttcagc taagcctaaa acttcgggtt tttgtaaaaa
     7141 aggggctaat ttttttgcat ttaaatttgc accattggtc tcaaaagggg ttacaggtac
     7201 acaagaaggt agcataaaaa agatattcat gagtgtttgt cttgcatctt ctatcataaa
     7261 ctcaagacct gtactcccag caatgtttcc tatttcatgg ggatctgtca ctacagtagt
     7321 aactccatga agcaaggata cttttgaaaa ttcgcttggt gataaaagtg agctttcgat
     7381 atgcacatgg ccatcgatga atcctggcac tagatattgc ccattagcat tatgtacgct
     7441 ttttccttcg tattcaccga ttcctacgat cattccttcg cagattgcta catctttttc
     7501 catgatttct ttcgtaaaga cgttgacgat cttagcatgc ttgatcacta ggtcagcagg
     7561 ctttttttta cttgctactt cgatatattt ccttaatgtt gtttccaatg gagtgcctcc
     7621 caagataaat ttttataagt ataacataaa ttcggttttt gccaatattt ttttgtaaat
     7681 tcattttatt tgaaacaaaa agatcaaaaa gacgtatgtt ttttgtggag gggagaataa
     7741 tggaaataaa acgattatcg gatcaaatga taagtgaaag ttttgccaaa catgcccaag
     7801 acgtatatat attgccagct tcagaagtat tttacatcta ttttcgctat ggaaaaaaac
     7861 gaacattctg gaagaaagtc tcttcggcag atggacagca actgatctca agatttaagt
     7921 atttgggaca gatggatatc ggagaaaaaa gaaaagctca gttaggtgca tttacttatt
     7981 ctttagaaaa acaggaagtt cgtctgcggt tgtcttctgt aggagattac cttcaacgag
     8041 agagtttagt gattcggcta ttatacggta cttttgaaac accccatcgt tgctttagtt
     8101 ctcgagattt tgcaactatt ttacagaaaa ccgtcagacg tggattatac cttttcagcg
     8161 gaccagtcgg atcaggcaag acttctctaa tgtatcggtt ggctttggaa gaaaaattgc
     8221 aagtgattac tatcgaagat cctgtagaga tagaagaacc gttttttctg cagctacaga
     8281 tcaatccgaa aatagcacag acttatgata cacttttgag actcgcatta cggcatcgtc
     8341 cagacttgct aatcattggc gagataagag atgaatcaac tgcacatgct gcggtaagag
     8401 cagcactaac agggcaccgg gtttttgcaa ccgtacatgc acgcaatctt gaaggtacat
     8461 tggtcagaat ccatgaactg acaaagaaaa aagaagagct atctgaatgt ttagctggtg
     8521 tcatttatca agaacttttg gaagatgcca atcaccatcc ttctgtttta tggagttatc
     8581 aatttatgga ggaagaatca gtaaaaaaat catgggagga aagttatgaa gaagctcagg
     8641 aaaatatttg gcagtcagaa ctattctaaa ggattaacaa gaagccagca aaaccagttt
     8701 gcacaaacca tgggagaact gcttttatct ggttttagtc tgcaagaatc cctttattta
     8761 ttactcacga tccgttcgtt tccacctgtt attttggcaa atatccaaca tcagctaaag
     8821 gaagggcaag cgtttagtga ggtactttcg caaataggat atggagcaga gcaactggta
     8881 cagatcgagt tagctgaacg tcatgggaat ctgccgacaa ctttgttgga aattgccaaa
     8941 cagtttcggc ttgttgaagg atttcgcaat gatttgaaaa agctgcttgc atatccgatg
     9001 ctattgctca cctttttgct aggtatcttg tttagtatgc ggttgttcgt tttgcctcag
     9061 cttattggtt caggaatggt catgcaagag cattggggta ttcagctgat acaagtcttt
     9121 cattggtatg ttctactatt catcctagcg gttactctat tggctttact agtaaggtat
     9181 cgtctgatga agttgtctcc tgttgatcag agtgaatgga taagcagaca attattgatt
     9241 ggaccgatat attccttgta tcaatcggct tatttgtcac tagagattgg aaagcttttt
     9301 catgaaggtt tcgaattaag gcaaatcata gtctgcttaa aggaaacgaa acaagaaagt
     9361 ctgattcagc atcttgcctc taagttgatt caaggattgg aggcaggtat gtcattggct
     9421 gagcagttcc agaattatcg ttttctatca gaagaattca gtaaaattgt tttacaggga
     9481 gaggcaaagg gaagtttagg taaagaactg attttttaca gcgaatggac aagaaaacag
     9541 cttttcaaac gtcttcatca ctggatgcat tttattcagc caatcatttt tttgggcgta
     9601 gcggctttga ttttattagt ttatgccgct gttttattac ctgtttacgg aagcatagag
     9661 gaggttttgc catgaaaaag agaaaaatat cttattatag cggttttaca ttgatcgaaa
     9721 tgctaatcgt cttactgatc atcagtgtat tagtgttgtt gttcgtccct aatttgtcgc
     9781 gttataggaa tcatgtagac caagaaagtc gtgaagcgat catccagttg gtagacacac
     9841 aaaaagagct ctatgcgctt caaaataatg gaagagttcc tactgtggag gaattgttga
     9901 atgaaggcta tatcaaacga gaacatgcag aaatctatca gcggccttga aggtaaggtt
     9961 ttagcatcag gatatacgtt gtttgaatca ctcttactga ttttattact tacaacgatc
    10021 ctttcttttc cagttctcgc attttctgca tggaagaatg aattggcagt ctatcaattt
    10081 ttcggccaat ttgaaaaacg aatctattct acgcaaaaaa tcgctattgt gaatcagatc
    10141 caaacaggct tttattggaa tagtgaagag aatcaaatca tttttcatgt tccgaatcca
    10201 gataaaataa attgggaagt attagatatt cctgatgaga ttaccttgaa acgccatgct
    10261 tctattactt ttgctgctag aaccgggaat gaaagttcac taaaagctta tcaattttat
    10321 tgggaagaaa aagagcggac gattacgtat caattccaga tagggagtgg tcgctataca
    10381 aaaagaattg aataaaacag gatttgtttt gttggagtcc cttacagcgc ttatgatcgc
    10441 ttctgtctgt gtctacttag tgagctttgg ccagctgttt ttgcttcagg cagaaaagaa
    10501 aagtggggag gaactgctat ttattcgaat gatttatgaa gatatcaaga cgcagcggat
    10561 gtatcacaaa agaccagagc gaagggaaga aatgatccca tacgatatcc aaattgtcga
    10621 taataatgga gtaatcgaaa aagtgtttgt caatatggag aaagaagcga tcagtattga
    10681 aaaaacaagt aattccagca tttacgctga ttgaatgtct aattggactg atgatattga
    10741 gcagtctatt gctcacattc aatcttttga ttcagcaaac catccaagta agtgaatttt
    10801 tgcagcgtaa agatcaaaaa gagtggctaa tatttttagc acaattagaa gaagaactca
    10861 agagcagtca caatgtctct gtgaaaaatc agcaactgca ctacacgatc gaggataaac
    10921 agtatatcat cgaaagatat caggcaatga tacgtaaaag aagaacttcc ggcggacatc
    10981 aacctatgtt aacaagtatc actgagcttc aattattgga aaaagaacac acaatttctt
    11041 tttatgtaca ctttgaaaat ggagaagatg gatatgccat atggacaaaa aacgatcagt
    11101 agaaaatgtt ataagggctc tattttattt tctgtcttgc ttttatttct tttatgtacg
    11161 tttttattga tggcagcatt agaaagctac cgtttgtcaa tggatcttaa tatacgaatc
    11221 aaaaattatt atatagcaaa aataatgaag cagatggctt tatcggaaca gtctacgctt
    11281 gccgaaagtt cggaaggagt tttttactac acaactggat ctgtaacata tcagtgggta
    11341 caacagtccc tattcttaga ggtggaagtc tcaccgttta tctttcggtt cacagaagaa
    11401 gtaaaaaatg atacagatac tagtacagaa tgatggaatg ttcgtgaaaa cgccctcttt
    11461 tctgttataa cactgttttt aataaaaaaa tgtttgccgt ttcacaaata aaaaacagtg
    11521 aaaacgattg aaattttgtc aattgttcga tagaattaaa caggtgaaaa aattgttata
    11581 atatatctag aaatgaggtg caccatgtta ccagaaaaaa tggaacaagg gtttaatcaa
    11641 aacctggaag ctattcagct tttacagaat gcattgggaa cttccttttt agaagcctat
    11701 gtcgaaaatg cagaaaattt aatagatgat tatcaagtac gtgtggtgga tggagtgccg
    11761 acaaaagaga caacgcaacg gatcacagct ctttatgaag agttgaagaa actttctttc
    11821 gagccagaag aatggcgcag actgtctcaa ttacttttat tgaagggcag ccagacagaa
    11881 catttgcagg cgaaccatca actcactcct gatagtatcg gttttttatt tgtttttctt
    11941 attgaacaac tatacacaaa taaaaaagca ccggttaaga ttctggatat tgcggcagga
    12001 atgggcaact tgttattaac agttctttta aacctatcga atgcggggta ccaaacagaa
    12061 ggaattggtg tggatattga tgatacattg ttggctgtcg cagcttctac aagtgatttg
    12121 acacaagcaa atgttcaata ctttcatcaa gacgggttgc aggaattatt aatagatcct
    12181 gtcgattttg caatcagcga tttgccaatc ggttattatc cgaatgacga aaaagcaaaa
    12241 gagtttttaa caagtaccga agaaggccat agttatgcac atcatttatt gctagagcaa
    12301 tcaatgaaat atgtgaagcc ggatggtttt ggtttgtttt tgatgccgag tggattttta
    12361 gaaacggatc aaagtgaaga aatcaaaaaa tggtttaaag aagaaggtta tcttcaagga
    12421 atgattcaat tgccagatga attgttccgt aataaacaat cgcaaaaaag tattttgatc
    12481 ttacagaaaa aaggaccaca agcacaacaa gtaaaagaag tcttgcttgt gaaacttgct
    12541 tcattaaaag aaccagagaa ggtgacggaa tttttcaacg agtttaaaaa ctggaaatca
    12601 tctgctctgt aatcattcaa cgagaaggag agtcatatgt ctaaaacaat tgcaatcaat
    12661 gctgggagtt caagtttgaa atggcaatta tatcaaatgc cagaagaaac cgtagttgcg
    12721 aaagggatcg tcgaacgtat cggtttacaa gattctattt ttaccatcaa gtacggcgat
    12781 aatcaaaaat tcgaacaaat catggatatc gaaaaccacg aagtagcagt aaaaatgctg
    12841 ttagatcagt tgattgaact gcaaatttta agctcttatg atgaaatcac cggagtaggg
    12901 caccgtgttg ttcacggagg agaaacttat ggggattcag tagtgatcga tgagaaagta
    12961 atggaccgca ttgctgaatt agcagaattt gctcctttgc ataatcctgc aaacttaatg
    13021 gggatcaaag cgtttaaaaa gattttacct gacattttga gtgtggctgt attcgataca
    13081 tctttccact caacaatgcc aaagcaaaat tatttatata gcttaccggt gtcttattat
    13141 gaagatttca aagtaagaaa atatggcttc catggcacaa gtcatcgtta tgtgtcggaa
    13201 cgtgcggcaa aattattgaa caaaccgatc gaagaactaa aaatcatcac ttgccactta
    13261 ggaaatggtg tatcgatcac agcagtcgat ggaggtaaat ccatggatac ttctatggga
    13321 ttcacaccat tagctggggt gacaatgggt acacgttcag gtgatatcga tccagctgtt
    13381 ctcccatatt taatggaaaa attagagatt gatattgatg aaatgatcaa tgttttgaac
    13441 aagaaatctg gtctgctagg cctaacagga ttgtctagtg atatgcgtga tcttgaaaag
    13501 aactatgaaa aagaacatat tcgtttagct tatgacattt tcgtggaccg catccgaaaa
    13561 tacatcggcg gttatgtgac aacgatgaat ggtgttgatg caattgtctt tactgctggt
    13621 atcggcgaga atgacggaca tgtacgtagt gaaatcatca aaggtatgtc ttggtttggt
    13681 tgtgaaatcg atccagcgtt gaatgaattg cgtggtgaag aagtagatat ctctacaaaa
    13741 gaatcaaaag taagagtatt agtgatccca acagatgagg aactaatgat tgcccgtgat
    13801 gtggagcgtc tgcgcggcta agccaaagca ttcgttagga taaaagatga aacgcatgca
    13861 agagggaatt cttgcatgtg ttttttttgc ggagaagacc gcaaaatggt aatttaactt
    13921 caatttccct tcttttttac aggatcttct tagggaattt tgcttggaaa agtggtcaaa
    13981 agggaaaaga ctggcatttt atttattgat tttcaggaaa atcccaatat tttattttca
    14041 ttcatcttta taagggacat tacatatgta aaaagaaaaa acatttttag atcagcctcg
    14101 tgggctgtcg actttattct tcgctttatg tttagctaga aaaccgatcc gaaacattca
    14161 ttgatcgaat aataaaaact tgattttcca cctgtacttt gcctgaatcg tgaaagcaca
    14221 ggtatgaaaa tcaagttttt tgttgcgaaa aaacgtccta gtcaatcagt tggccgagcg
    14281 gctggcggct gatctgctcc tgatcaataa cgagtgcttc ttggaagtgt tgctgcaaaa
    14341 agtagcggtc atggctgact gctaggagcg ttccttgata gtcttgcagc atttcttcaa
    14401 tttcttctcg agcatagctg tcgaggtgat tggttggttc atccaaaatc aggacgttga
    14461 ttttttctaa aaagagtttc agcaacgcca aacggatttt ttcaccaccg gataaatctt
    14521 tgatccgttt acccacatcg ctttgataaa agccactttt ggctaattgc cgtcgagcga
    14581 cttcttcgtt tccagtcaat gtgatggcgt aagccagcaa acggctatcg ggatcggcaa
    14641 actgcagctg ctgcggcaaa taaccgattt gcaagctact acctaatttg atggtgccac
    14701 cgtaaggctt ttcctcaccg agtaataatt tgagtaaggt tgattttcct gcaccattgt
    14761 cgccgatgat ggctaaatga tctccacgat agactgcaaa actgctgtct ttgaatagta
    14821 cttgatcagc gtactgctgg tgcaagtttt gaatgatgaa aacttctttg ccagattttc
    14881 caccattaga caagctttgc agccgatttt tcgggggagg tggcggtttg acgagtgtca
    14941 gtttcgccag gcggcgttcc agttctttgg cttttttgaa gaaggactca ttgtctcctt
    15001 catgtgccca ctgacgaaaa cgacgaatca tgactttcaa ccgctggact tctttttgct
    15061 gcagttcgta attttttgtc agctcagcca agcgagcttc tttcaagact cgaaaacggc
    15121 tgtagtttcc gggatactca atcaatgcac cttcttccag ttcccatgtt tttgtggtga
    15181 cttgatcgag aaattgccga tcatgagaaa cgaccagaac cgattttttt gtattctgta
    15241 aatagttttc caaccagcga atactgttaa gatctaaatg attggtcggt tcatcgagta
    15301 aaagtaaatc ggcatcggct gctaaaattt tggctaaagc gactttaact cgttcgcctc
    15361 cgctaagttc tgttaaccgt gcttcctgac ggtgtggaat ccctaagcct tgcaagatcg
    15421 ctgcaatccg gtcttctaat acgtaaccgc ctagttcttc aaagcgatgc tgcagctcac
    15481 cgtaacgggc cagacacttc tctaagtttg tatctggttc tgtcatcaat gcttcgtagc
    15541 gactcatttg ctgtttgagg tcctgtatct cagcggcaga atggagcaag tagtggttga
    15601 cggtcgcttc ggaaactgta agttcttgtt cgaccgtccc gatctttaag ccttttttgc
    15661 gactgataac accgctgtcg acaccttctc tgccggtcaa gatttgtaaa aaggttgatt
    15721 tgccagttcc gttgataccg actaaggcaa ttttttcacc agcttcaatt tgacaggata
    15781 aatcctcaaa taatggattc ccgccgattg tttttttaat agaaattgct tgtatgatca
    15841 tgatctctct ccttgagacc actcaaaaag gccatggtta tccatggcct gtgttattta
    15901 aactcagatt atcatgagaa aaataccttg agatggccat tgattttacg tgaattcaac
    15961 tgttttggac aaacttgtcc cgtcaacagc aactgtaaaa ccaattgcat ggctaaatgc
    16021 gatatggtat cgttggtaat tttcattact tgttatttta gtcatgcgca tcatagtatt
    16081 ttccctcctt cgtgtagttg tcttttccag tgtacctgaa agtgtcagaa agttcaacta
    16141 gcgctgacat ttacttaata tttatcgatg tgtaattctg tcagagagct ttttttatct
    16201 gactgagggt aatgtctcgt ggaccacatc attgtattct gagctaaacc agcgttcagg
    16261 cgaaatcata tggtgtttga taaaaaactt tgtctacatg acttcctttt gccatttgct
    16321 taataccgtt gcattaaaca gctcttctct ttggaaatct caccaacatc gcgttcagga
    16381 gtatcgttgt ggttgcaatg gcaagcggta gcctttcatc aattcttgaa tgatttaccg
    16441 aatgtagcac ttgccttctc gcggtacccg aacccgaaat aaaattttag tttggcgata
    16501 aaatcggtat ccgccatcga tacttcatat tttttcgtat cagattcatc ggaaacttcc
    16561 acattgacga accaataagc tttggcatgc ttaggtcgtt ttaaaccaca ccagggcgac
    16621 taatcgccct ggtgttcact tttttttatt ttacttgaac tcattgactg ttttcccttt
    16681 attttaaaca ttataaagac cgatgagtgc ttgccgcgtc ttttgttttt atcacggttt
    16741 tttgtgcaaa aaaacctgcc gatagttggc aggtcgtcta cgtgttaagc tttggtgtgg
    16801 tctttagctg gcaattggtt ttctaaatca gttcggacaa caagcacatc gcaagctgcg
    16861 tttctaataa catattcaga aacagaacca ataaataaac gttctacagc attcaatcct
    16921 gtggctccta acataatcaa atctacttga ttatcttctg ggatttctct ggcaataatt
    16981 tgttttggtg agccatattc tacgacagag gttacattat tcaagccagc tttcttcgca
    17041 ttgctttcat aatctgccaa tgtttgcttt gccatttcag tagcttgttc tgcaagcatt
    17101 ccatcaaagg atgaaacggt ttggaaagaa cgtgtgtcaa tgacatgtgc taatagaagt
    17161 tctccgttat ttcggatagc tacattgacc gcctttttaa aagctaattc agcttcatca
    17221 gaaccatcta cggcaaccat gatttttttg tattgttgta acataagcat tcactcctca
    17281 agtcaatcgt attgtactct tttattgtag aacactttag ggaaaaagag aattatttac
    17341 tgctttggtt cagcaatttt ttttgcaaat cggatactga aggtggcaga aacaataatt
    17401 acgatcagta aaaagaccaa ggcaataaag cgttgattga aaaaagctgt taaaataccc
    17461 attacgccta aaacagcata tgcagctccg taaaattgta ggaatccttg atttttatca
    17521 tttttgttga tcaatacaaa aaagatatcc tgtttattaa ataaataata gctaacgaat
    17581 aataatacta aagtggcgat aatcattaaa atcctaatca tgaacaaatc ctccaaaata
    17641 agcaacaaat agaaaaagca atttgccaag agcaaattgc tttattcttt aaaagatggt
    17701 aatctccgtt aatgccgttg tttgtcctgt agtattgatc ccgcgaattc aagcaggtgg
    17761 gtcccatggt cgtagcttcg aactaccaaa tgataaggca tgttaccagc agtgacacaa
    17821 gacgggtccc aagatatcaa taaaaatgtt cggtcaacac acaaagatga caattcgaac
    17881 atcacagatt tcccacctct atagtagcat atgaatttaa aaagagcaat gagagagctc
    17941 gcagaaacag aaccgttttc ttgtctagtt ttttggagga tgctttttcc actcttttat
    18001 acggtaatac tgttgaccca gagcctgttc atatttccct gtatcttttg gctgataata
    18061 ctgagcattc ttcagtttat ctggtaaata ttgctgattg acccaagcct gatcaaaatg
    18121 atgaggatac tgataaccca cgccacggtt gagtgatttc gcacctttgt aatggctatc
    18181 tctcaaatga tcaggaacat ctccagcttt gccctctcga atatcagcaa gtgctgcatc
    18241 taatgccatg taggcagaat tggattttgg cgacaagcaa agatcgacga cgacgtctgc
    18301 tagtgggata cgtgcctcag gcaatcccaa tttttcagca gctaatacag cgttcactgt
    18361 acgagccgca gcagcaggat tacctaaacc tatatcttca taaccgataa ccatcagacg
    18421 ccggcaaata cttgccagat cacctgcctc aactaaacgg gcgagataat gaagtgcagc
    18481 atccacatca cttccacgaa tcgatttttg aaaagccgaa ataacgtcat aatgagcatc
    18541 tccatttttg tcatgggtaa gagcttttct ttggacacat tcttcaatga tcgacagtgt
    18601 aagatgaatc cttccttctt tatctgaagg agtcgatagt gtagccagtt ccagcccatt
    18661 caatgcactc cgcaaatcgc cgttcgttgc acgcgaaagg tgaagaagag catcttcgtc
    18721 taactggatt gcttgttgtc caaggccgcg ttctttatcc cttaaagcat gttcgactgc
    18781 caactggatg tcttgttctg ttaatggttt gacttcaaag atctgtgtac ggctgcggat
    18841 cgctggattg atggtaatat aaggattctc agtagtcgct ccgatcaaga tgattcggcc
    18901 actttctaaa tgaggaagca gaaaatcctg ttttgtttta tctaatcggt gtacttcatc
    18961 caataagagg atcactgtac cactcatttt tgcttcttcc gctacaactt gtagatcctt
    19021 ttttgaatct gtagcggcgt ttagcatgcg aaatgcataa ttagtagagc cggcgatcgc
    19081 acttgcgata ctcgtttttc cagtaccagg aggtccatat agaatcatgg aagagagcat
    19141 gcgagcatcg accatgcggc gtatgatttt tccttccccg actagatgct gctgaccaac
    19201 gacttcatcg atcgttcgag gtctcatgcg ataagccaat ggctgttgca taggattttc
    19261 ctccttcttg tttttacgtc ccttaattat atcatattta gcaagatgcc tagaaagaac
    19321 agccttctgt tgttttgttt tcatagctta tccaactaat attgtgtatg ataaagtagg
    19381 tgagaaaaat gacggataaa caaaagatag atgaattttt ttggaataaa acgacgccag
    19441 aagtggcacg ggatttattg gggatgtatt tggagcatga cacaccagaa ggaagattag
    19501 ctggatatat tgtagacgcg gaagcatacc taggtccaga agacgaggcc gcccatagct
    19561 atggtttaag aagaacaccg cgtgtgcggg caatgtatga aaaaccaggt acgatctacc
    19621 tttacacgat gcatactcat cggattttga acatcattac tcagccagaa gggattcctc
    19681 aaggagtaat gatacgtgca atcgaacccg ccgcaatgat tgatcaaatg agtaaaaatc
    19741 gtggcggaaa aactggtcca gatatcagca acggtccggg aaaattagtg gaagcattag
    19801 caattcctca agaactttat ggtcagtcta tagcagatag ttcgttacgc ttagtttttg
    19861 agaaaaaaaa gacaccaaaa aagatcaatg ctttgcctag aatcggtatc ccaaacaaag
    19921 gtgtatggac agaaaagccg ttgcgttttg ttgtgtcggg aaatccttat atctctttac
    19981 aaagaaaaaa ccaaatcgag aaagattggg gttggaggaa agaaaatgaa aaagaaggat
    20041 ctattaacat atttagatga aataattgaa aaacaagtga cagactatga tgtagcttta
    20101 gattgggata caaaaaatca tacgatcgag atcgttgttc gcttgtttgc agaaaataaa
    20161 gcacaatttg agattgatga tgcagaaggg atcgtttcgc aagaaccaat tatcgaattt
    20221 gaagacggcg tacttctttt taatcctcag aaatctgttt ttgatgaacg caattatcta
    20281 gcagtaatcc cttatgaagg aaaaaaaggg ttggcaaaat cagtagctga ttctttagtg
    20341 gaatatctaa atgaagtatt agctcaaggg caaagcgatt tgcttgattt tttggatgaa
    20401 gacgatgacg aagctgtatt cgagcttcac tgggataatc agcaactagc agaattagta
    20461 gaagtaaaac agcaaaatga agcagatact tatttaccgt acccaagtta ttaaggagtg
    20521 acagatatga aatggaatga agtaaaagta gaaactgaaa gtgaagcagt cgaagcagtt
    20581 gctaatattt tgatggaggc aggggcgagt ggtgtcgcga ttgaagattc tttagatgtt
    20641 gaacattttc agtcggatca atatggtgag attttagata aaaatgattt tacatcgctg
    20701 aaagaaggcg cactagtcat ggcttatttt ccggaaacga ctttcttacc ggaaatcttg
    20761 ccttttatca aagatcgtat cacacaatta cctgagtttg gtctagctat tggaaaaaat
    20821 aaagtaacag ttagtgaagt agaggaaagc aactgggcaa cagcctggaa gaaatactat
    20881 catccggtgc gtattacgcg aatgctgacg gttgcgccaa gctgggaaaa ctaccagaca
    20941 gaagatcctc tagaaaaaat tatcacacta gatcctggta tggcatttgg cacaggcact
    21001 catcccacga cttccttgac attgacagca ctagaaacag tcttgcgtgg tggtgaaaca
    21061 ttgctagatg tgggaactgg gtcgggcgtg ctaagtattg cagcaaaata tcttggtgca
    21121 aaagatgtct atgcctttga tttagacgaa gtagccgttc gttcggcgaa agaaaatatg
    21181 gatatgaatg aaatagccaa agatgtccat gtatcagcta acgatctttt gacaggaatc
    21241 gagattgaaa gtgatgtaat cgttgcgaac attttagcag atatcatctt attgatgatc
    21301 ccagatgctt ggcgtttact gaagcaaaca ggcacgctga tcgtttcagg aatcattgaa
    21361 gagaagaagc aaatggttct ggatgccatg ttagaacaag ggtttatcgt ggatcaaatt
    21421 ttccagcaaa aagactggta tgcaattatt ttgaaaaaac cagaggtgga atagatgcag
    21481 cggtattttt taaaagaatc atatgaatca aaagactatt ttgttttaga aggggaaaac
    21541 tatcaccaca ttgtacgggt tatgaggatg gaacccgagg atgaagtttt tttggcattc
    21601 aatgatcaaa tagccattgt tgcagaaatc acggagatta ctgaagaggc tgtttatctg
    21661 aaagaaatta aaaaagaaga aaatcaaaag gaactgccaa gttcgattac catagcgagt
    21721 ggctatccta aaggggaaaa gctggattgg atcgttcaga aatccactga attaggagcg
    21781 caagcttttc ttggtttccc ggcatcagcc tctgtcgtta aatgggatgc aaaaaaacgt
    21841 atgaaacgac aacaacggct tgaaaagatt gctcaagagg cagcggaaca atcccatcgg
    21901 cagatcgtgc catcagtaga gttattggaa acgaaacaag atttatttga gcggctagat
    21961 gattatgacg tggtcttggt tgcttatgag gaatcagcaa agcaaggcga aaaatcaaat
    22021 gcagccaaaa ttcttagtga aacaaaacca gatagtcgaa tcttagctgt ctttggacca
    22081 gaaggtggat tatcccgcga tgaagtcgat tattttgtag aacacaaagg gatactttgt
    22141 ggattaggac caagaatcct tcgtacagaa acagcacctc tttatttatt aagtgctgct
    22201 agctatcaat gggaattaat gaatccttct ttcagctaac gttcttcatt aataaaagag
    22261 tatcgacaga aagaattgaa aaaatagtca acaatttgta taatagaaac aattatttat
    22321 acaatggata cgaaaaatcg tttgaaaaag cttttaaaaa gcgaacattt ccgtaaaaaa
    22381 acgtataact gcacggattt tgggacaatt atcaaacaga tatttgtccc gattcccgtt
    22441 ttaccgaaaa aagaaagggg gatcgattcc tgtgacaaaa gaagaaattt taacgggacc
    22501 cggtgtcata aagctcgtta gtcaatacat gggtcccgaa catgtagcat ttgttgagaa
    22561 agcatgcgaa tacgcgactg ctgcacatga cggacagttt agaaaatcag gcgaacccta
    22621 cattattcat cctatccaag tcgcaggtat attagcagat ttaaaaatgg atccccatac
    22681 agtggctaca ggcttcttac atgatgttgt tgaagataca gaaatcactt tagaagatct
    22741 gagagaagaa tttggcgatg acgttgctat gttagtggac ggcgtaacca aattagggaa
    22801 aataaaatat aaatcccacg aagagcagct agcagaaaac caccgaaaga tgctgctagc
    22861 aatggctcaa gatttacgag ttatcatggt caaattagct gatagactac ataacatgcg
    22921 tacgttgaag cacctgcgag aagataagca aagaaggatc gctcaggaaa ctttagaaat
    22981 ctatgcgcct cttgcacatc gtctagggat cagccggatc aaatgggaat tagaagatac
    23041 agcacttcgt tatctaaatc caaaacagta ttaccgtatc gtccacttga tgcagacgaa
    23101 gagagaagaa cgtgaaaaat acgtaagtgg tactgttgaa gatattcgaa tagcgacaga
    23161 agagctgggg atttttgcag aaatctatgg acggccaaaa cacatttatt caatctatcg
    23221 taaaatgaaa gatcagaaaa agcagttcaa cgaaatttat gacttgctag ctattcgagt
    23281 gatcgtagat tcaatcaagg attgttatgc tgtattagga gcaatccaca caaaatggaa
    23341 accaatgccc ggcagattta aggattatat tgccatgcca aaagctaata tgtaccaatc
    23401 tttgcatact accgtgatcg gtccggcagg aaatccggta gaaattcaaa tccgaacaca
    23461 ggaaatgcat gaaatcgctg aattcggggt tgctgcacac tgggcctata aagaaggaaa
    23521 aaatgaaaaa gtagaaccag atggtatgac gaaacaatta agctggttcc atgagatact
    23581 cgaacttcaa gacgaaagct atgatgcttc tgaatttatg gaaggcgtaa aaggagatat
    23641 ctttagtgat aaagtctacg tcttcacacc aaaaggagac gttactgagt taccaaaagg
    23701 atccggacca ttggactttg catacagtat ccatacagat atcggtaaca aaaccactgg
    23761 tgcaaaagta aatggcaaaa tggtgcagct tgattacaaa ttgaaaaacg gagatatcat
    23821 tgagatcatg acttctccaa attcatttgg cccaagtcgc gactggttga aattagttgc
    23881 tactagcaaa gcaagaaata agatcaaacg tttcttcaaa gcccaagatc gagaagaaaa
    23941 tgtgatcaaa ggccacgaat ccgtggtcaa atgtattaca gatctaggat ttacgcctaa
    24001 agatattttg acgaagaaca aactgcaaga agcactcgat cgttttaatt atcaaacaga
    24061 agatgatctc tatgcagctg tagggtatgg agaagttagc cccttgacga tggccaatcg
    24121 tctgactgaa aaagaacgta aagaacaaaa aatcgagcag caaaagcaag aagcagaaga
    24181 aatcatgaat cagccgaaaa aagaacctga caaaatgaaa gtacgtcatg aaggtggcgt
    24241 tgtcattcaa ggggtagaaa acttactaat tcgtatcagt cgctgctgta atccgattcc
    24301 tggtgatgat atcgttggtt atatcactaa aggcagaggg atatccattc atcgtcgaga
    24361 ttgtccgaat gttcagcctg acaaaccaaa tgtagcagaa cgtttgattg aagtcgaatg
    24421 ggaagataca tcgaatacac gaaaagagta tgatgcagat ttggaaattt acggctataa
    24481 tcgttcaggc ttattgaatg atgtacttca aacagtcaat gcgctaacga aaaatctcaa
    24541 cagcgttgaa gcacggacga ataaagataa aatggcgacg atccatttga cggttggtat
    24601 ccagaattta tcccacctaa agagtatcgt ggataaaatc aaagcagtac ctgatgtcta
    24661 cagtgtacgc cggacgaatg gataggagga cagatatgag agcagttgtt caacgggtaa
    24721 gtaaagctgc tgtgtcagtc gatcagcaag aaattggaaa aattggctac ggcctacttg
    24781 ttttattagg cattcatgaa accgatacgc aaaaagatgt ggactatctg gtaaaaaaaa
    24841 ttgcacaaat gcgtattttt gaagatgaac aaggaaaaat gaatattagt gtagaagatg
    24901 tgggaggaca aattttaagt atctctcaat tcacactgtt tgcggatacg aaaaaaggaa
    24961 atcgaccaag ctttgtggaa gccgcacgac cagaaacagc tattccttta tacgaatcat
    25021 tcaatgaagg aatcagaagt cgcggcatca cagtcgctac cggacaattc ggagcagata
    25081 tgtcggtttc tctcgtcaat gatggacctg taacgatcat tattgattct aaaaacaaat
    25141 aaagtaatct ggttttcact agtaaaaact atgaaacacg tcgcttaagt tagactgata
    25201 aactctaact taagtggctt ttttgtctgc aaaaagcgct tcttttgtca gcagtcggaa
    25261 aaaaagttaa gcttaaatta tacaagttat tttttatagg aggttgctga agatgaaaga
    25321 agtaccagta gtagatatca aaatcacaga tttaaaaaaa ttgtatcaaa aaggatacaa
    25381 catgttggag gagttacgcc atgaagcgga cgctcccgtc gtcaaagcaa agattttcaa
    25441 taaagaagct atcacgatct acggatcatc agcagcaaaa gtattttatg atccaaggaa
    25501 tttcaaacga aaaggtgcta tgccaaaatt agtattgaag acactttttg gtcaaggtgg
    25561 agtacagact ttggatggag ctgctcatca ccatcggaaa aatattttta tggatctaat
    25621 gacaccggaa agaatggaag attatcatcg tatcttagat aagaatctga cacaagcact
    25681 tgaagcacag catggtcaat ttgaactatt tgatttaagt aagatggttt tctttacatc
    25741 tatttgcgag tgggcaggta tcaatttatc agcgatttct aaagatgagg tcgaaaaatt
    25801 agcagaatat caaatctcta tgatcagcgg aacgtttact tctccgattg atcacataaa
    25861 aggtgtcgaa aatcgcaaaa aatcagaaaa atgggcgcaa ggcttgattg aagaagctcg
    25921 acaaaatcct gtggctggaa aagaaaatgt ggcattatat gcttttgcca atgccacaga
    25981 tttagatggt cagcttttac ctctggaagt ggcagctgtt gaattattga acatcatccg
    26041 tccaacggtt gctctaactg tgtgggcggc attgatggga catgcacttt tcagtagacc
    26101 agatttgtat cagcaattaa aaaatgactt ttcgacgtta caagatccgt ttattcaaga
    26161 aatgagacgc tattatcctt tcttcccaat gctacctgcg atttcgctga aagaagtaga
    26221 agtagatggt tatcgtatac cagaaggcag ttgggttatt ttagatttat atggaacaga
    26281 ccatgatgaa cgaacagttg aagcgccaga ttcctttatg atcaaacggt atgtagggaa
    26341 agcaaaagat atttcctata aagaagaata tgagatgatt gcacaaggcg ggggaaattt
    26401 tcgacaaatg catcgctgtg caggagaatg gatcactctt catagtctac gtgtgttttc
    26461 cgatcagcta gtaaataagt ttgagttctc tgtacctgaa caagattgga cgattccatt
    26521 caatcagttt ccgacttatc cgaatagccg agcgctactt tacaagaatt agattagcat
    26581 aaacttatcg aaaaaaagga agatcaaaca gtgctgatct tccttttttc atattactat
    26641 aaattgataa ggtcaattga tcaaaatcca cgaaatgctt catcaatggt ttgataatct
    26701 tcattggata aatgcaaatc taaagcttgg acattgtcag atacttgctc tggacgctta
    26761 gctccaggta taactacaga cactcgtgga ttcttcatgt accaagctaa aacaagttgc
    26821 gcaggagtgg cttggtatct tttagcaatg gggcgaagta catcgacagc ggttaaaatt
    26881 tctctgaaac gttctccttg gaaatctgga tttctttttc gaggatctcc ttcaccaaat
    26941 tgtttttcct caccaagttc atattttcct gtcagcaaac cggaagcaag aggaaaatag
    27001 ggaacaaaac tgattttgtt tttttcaaga taagggaata attctttttc agcctgccga
    27061 tggatcaaac tgtatttatc ttctactaca tcgacatatc catctgcatt cgcttctttc
    27121 aattgttcta acgtgaagtt tgacacaccg actgctcgga tttttcctgc ttcttttaat
    27181 tcgtgcaatg tagcaactga ttcatttttt ggtgtgcttt catccggaaa gtggatataa
    27241 aaaatgtcga tgtaatcagt ttgcagacgt tttagtgcat cttccaccga ttgtttgaga
    27301 aactcaggag aattatcaaa tgttcgccct ttatttggta catgagcagc ttttgtagcg
    27361 atgacgacac gtgagcgatc atattctttt tccttcagta cctcgccaat cagttcttcg
    27421 gaacggccgt ttccgtaagc ataagcggta tcgatcaaat tgatcccgct attcaaagct
    27481 gtacgaacta cttgttttcc tgtctcatct tccaaaccgt caaaaagatt atgtcctcca
    27541 acggcatttg ctcctaatcc aagctgttct gcgtagactt gtgaatgtcc gattcttatt
    27601 tctgccatag ctgattcctc ctttttgtct agtctaaaac aaaataccaa aaagctgaac
    27661 taaaacgttt ttgtgagggg aaatagtcag atatctcttg actttgagac tggtttttag
    27721 taggttataa gtaatatcga atgtcaatga aagagcgagt aagtttttga aactttacaa
    27781 gagaggatca tcgaggctga aaatgatcta aagtttcatt agcctaaaga cactctggag
    27841 atggacgagt gaaaagccgt cccgcgtacg agcgttattc gtaagaggaa agagaaatct
    27901 ttcaaaatta ggtggtaccg tgtatgaatt tcattcaccc ttgttcatga tgaacaaggg
    27961 tgattttttt atttacatag ctaagaaagg gagagaaaac tatgagtttc caaagaccaa
    28021 aaggaacaaa tgatattttg ccaggcgaat cagaaaaatg gcagtttgtc gaagaaacag
    28081 cacgtttatt attcagagat tatcagtata atgaaatgcg tacaccgatt tttgaacact
    28141 atgaagtgat atcccgaagt gtaggggata caacggatat cgtttcaaaa gagatgtatg
    28201 atttttatga caaaggggaa cgccatgtga cgcttcgacc agaaggtaca gcaccaatcg
    28261 ttcgttcttt tgttgagcat aaattgttcg gcccagagta tgcgaaaccg ttcaaaactt
    28321 tttatatggg accaatgttt cgctacgaac gccctcaaaa aggacggtta cgtcaattcc
    28381 atcaaatcgg agtagaagca tttggatcag aaaaccctgc aactgatgtg gagagcatgg
    28441 tgatggcact tgatttcttc aaacagttgg ggatcacgca aatccggcta gtcatcaatt
    28501 cattaggtga taaagaaacg cgtcaagcct atcgtcaagc attgatcgac tatcttttac
    28561 cttttgaatc agagctgagt gaagactcta aacgccgcct tcatgaaaat ccattacgcg
    28621 tattggacag taaagacaaa cgagatcaaa aatttgtggc agaagctcca tctatcttag
    28681 attatctaag cgagcctgct caaaaacatt ttgataccgt tgtttccatg ctggatgcat
    28741 tggctgttcc ttatgaaatc gacagtaata tggttcgcgg actagattat tacacacata
    28801 cgatttttga aatcatgagt gacgcaccaa aaatgggggc acaagcaacg atttgcgcag
    28861 gcggacgtta tgacaatcta gtggaagaac taggtggtcc ttcaacacct ggcttcggat
    28921 ttgcaatggg tatcgagcga ttactcttga ctatggaggc agaggaagta gttatccctg
    28981 catttaatga gttggatgct tacgtggtcg cactcggaga cgaaacaaat atcgaagcat
    29041 tgaaagtggt ccaagcgatt cgaaactttg gtttttcagc tgaccgcgac ttcatgaacc
    29101 gtaaagcaaa agcgcaattt aagacagcag ataaagcgaa tgccaaatta gtattggtga
    29161 tcggtgaaga tgagcttgct agtcaaacga ttaatgtcaa agcaatggca agccgaaaag
    29221 aaaaaagttt tccattagaa gagatatatg aacattttga tgaagtctat gatgaaatga
    29281 ctttattcga gtaggaggaa aaaaataaat ggcacaaaga acgatctatt gcggattagt
    29341 ttccgaacaa caattagaac aaacagttac attaaaagga tgggtacaaa aaagacgaga
    29401 ccttggggga gtaatcttta tcgacctacg tgatcgtgaa ggaatcgttc aaatcgtctt
    29461 taatcctgaa atcaacaaag aagcatggga aatcgcggat aaatgtcgca gtgaatacgt
    29521 gatcgaagtg actggggaag ttagaagacg cggagaaaat gcagtaaatc ctaaaatggc
    29581 cactggtgaa ttcgaagtga ttgcaagtga aatcgtgatt ttgaatcaag caaaaacccc
    29641 gccatttcaa attgaagatg atcaagcaat cagtgacgaa atacgtttga aatatcgtta
    29701 tttggatctg cgtcgtccaa aaatgactaa aaacatcaaa ttaagaaatg cgacaacaaa
    29761 agcgattcgt cattatttag atgataatga ctttctcgat atcgaaacac cttacctagg
    29821 gaaatctaca ccagaaggcg caagagacta cctagttcct tcacgtgtcc atgctggaca
    29881 tttctatgcg ttgccgcaat ctcctcaatt gtttaaacaa ttgctgatga atgctggatt
    29941 cgaccgttat tatcaaatcg ttcgttgttt ccgcgatgaa gatttacgtg gagaccgtca
    30001 gcctgaattt acgcaagtag atattgaaac aacgttctta acagcagaag aaatccaaac
    30061 ttacacagag ggattgattg cgaaggtaat gaaagaagtt cgcggtatcg aagtcacatt
    30121 gccatttcct cgtatgacct atgatgaagc aatggctcgc tacggcagcg acaaaccgga
    30181 tacacgtttt gatatggaat tgatcgatct aagcgataca gtcaaagaag tagaattcaa
    30241 agtcttccaa atggctcttg aaaacggcgg tgtcgtgaaa gcattgaatg ctaaaggcgc
    30301 tgcagatcgc tactcacgaa aagacatgga tcagttaggt caatatgtag gacaattcgg
    30361 agctaagggg cttgcatggc tgaaagtaga agaagacgga ttgaaaggtc caattgctaa
    30421 attcatggga gaagcaacag aagcaatcat taaagcaaca gatgcaaaac caggtgactt
    30481 gctgatgttt ggcgcagata aaagtgaaat cgtcgctgct gcattaggtg ctatccggac
    30541 acgtttagga aaagaattag gactaattga tgaatcgaaa ttcaatttct tatgggtaac
    30601 tgattggcca caatttgaat tttcagaaga agaaggtcga tatgtctctg ctcaccatcc
    30661 attcacgatg ccaaaagaag aagatatccc gttattggct gaagaccctg caaaagtcta
    30721 tgcagaagct tatgatatcg tcttgaacgg ttatgaacta ggcggaggtt ctatccgtat
    30781 tcatactcgt gagcttcaag aaaaagtact agaaactcta ggctttacaa aagaacaaat
    30841 ggaagaacaa tttggtttct tgttaacagc tttagattac ggattccctc cacatggcgg
    30901 tattgcatta ggattggacc gttttgcaat gttattagcc ggagaagata atattcgtga
    30961 agtcattgcc tttcctaaaa acggaaaagc agcagatgtg atgagtgatg caccaagtat
    31021 ggtttcgcct ttacaattgt tcgaattgaa tattgatgta acgaatatag aagaatagcc
    31081 gataaaaagg taaaaaggat ttgtcgaata cgcttctttt gacaaccttg atttaattga
    31141 aaaacacttt ataatgaagc cataacatat aaagcggggc ttttccatga aaaaaacatt
    31201 cgaaaattgg gcttatcatg attacacgac caagctttcg gtatctatcg tttatgcgtt
    31261 gatcgcatca gtcgcattga atttctttta tcagccagga catatttatt caagtggtat
    31321 tactggtcta gctcagattt tgacgacttt gtcaaaatca ctgataggat tcgaagtacc
    31381 ggtatcaaca actttgtatt tactgaatat acctttgttt gtattggcgt ggttaaaaat
    31441 cgggaagaag tttacaattt tcacttttat cacagtcact ttgacctccc tttgtatcca
    31501 tattttaccg gaaacagtcc tatctaccga tccaatcatc tgtgcgttgt ttgggggggc
    31561 tgtgatggga ctaggtattg ggctagcgtt gagaaacggc ctatcatcgg gcggtctaga
    31621 tatcgtgagt atcacgattc gtaaaaaaac aggaaagtcg attggttcta tttcgatcta
    31681 cttcaatgca ttgatcgttt ttgttgccgg aattctattt ggctggcaat atatgtttta
    31741 cagtacgttg tctattttcg tcagtggaaa agtaactgat gtcgtttata ctaaacaaaa
    31801 gaagatgcag gtaatgatcg taacgaaaca tccagaaaaa gtagtaaatg aaatccaaaa
    31861 ctgtatgcgt agagggatca cgatcatcaa tgaagcagaa ggtgcttata agcatgataa
    31921 gcagacagta ttgatgacga tcgttacacg atacgaactg cctctactta gagaagcaat
    31981 gaaagcttct gatccacaag cttttgtaag tattgcagaa aacgtccaga tacttggtcg
    32041 tttctatgag gatggcattt agatcgagtt tgacaaaaat tcatgaaata ctgctgtaga
    32101 cagacgttcg tttgtctgca gtttttttct accatagtag aatcagcaaa gaaaaactgc
    32161 ttttttattt gaaattttct taaagttgaa aaaaagcatg tttatttttt taaatttatg
    32221 ggatgataga aaaaaatagt agagaaagga cacgattcta cttgctattt tcacttaata
    32281 cccgtatatt acgaatgaaa aaagtttatg aaaagttgga atattgaatg gaaaaacaaa
    32341 aaaggttaaa aaacagccgc tacatcactg ggtttgatgg gctccgttca ttagctgtgg
    32401 tgggtgttat cttctatcac cttctgccaa ctagtatgaa aggcggctat ttgggcgttc
    32461 cgattttttt cgtcgtatct ggttatttga tcacggatct gctgcggcag gaatgggaac
    32521 aaaatggaaa aatcaatatt tggcaatttt atgtcagaag aatgaagaga ctctatccgg
    32581 cacttgtttt tatgcttgtg acagcttcgg cttatatcac tttgtttcag cgagggctgt
    32641 taaacaatct tcgaggagtt gtggtcagca gcttactgta tgtcaacaac tggtggcaaa
    32701 tcaataacgg tttatcttat ttcgatcgat ttgctaatga gtcaccgttt acgcatatct
    32761 ggtctttagc agtagaaggt caaaactatt tggtttggcc gatcttgttt gtattgttga
    32821 tggtatttgt tcgtaaaaga aaatggatct tttatactgt gttaggtaca tccttcctct
    32881 ccgccatctt gatgatgatc ttgtatacac caggaggcga tccaacccgt gtttattacg
    32941 gtaccgacac tcgtctgttt tctatatggt tgggaagtgc gcttgcgttt gtttggccaa
    33001 gtacgcgttt aaagaaaaac atcccgaaac aagcgaaaaa tattctgaat atcgctggag
    33061 gaatctcgct tttggcactg attctttgct tcttcttttt agatgatcat tacaatttca
    33121 tctattacgg aggaatgctc ctagtgagtc tgttatgcgt agtgctagtt gcagtgacag
    33181 cacaccccgg agcaagtttg aatcgatggc tgacaaatcc agtatttact tggattggca
    33241 aacgaagcta tggcatttat ctgtatcagt ttccagttat gattttttat gaagcaaagg
    33301 tgactgatat tgccgatcat gttttgttgc atactttagt ggaaatcaca ttgattctaa
    33361 tcatcagtga actttcttat cgatatgtgg agcgtccgct agcacgtttt gattatggtc
    33421 gtacattcca agtgatgaaa ggatggttca gcaaaccgat catcagtaag agcaaaccat
    33481 gggttgttcc aggcacatta cttgtagcaa ttgcactcgt tggtttcgtt acggcaccga
    33541 aaaacgctgt aacagccgat caaaaacaac tgcaggcaaa aatcgctgaa agtaaaaaat
    33601 tagcagaaga aaccagaaac aattcagaaa acaccactag ttcggttgac caagcgatcc
    33661 ttgataagta cgagttgtcc aaggcacaag ggaataaggc acaaaaatta gaactaacgg
    33721 catttggtga ttcagttatg ttggatgctg caacggatct acaagaactt tatccaaaag
    33781 tagtggtaga tggagatgtc ggacgacaat tatatgccag tgtaccgtac attgaaaaat
    33841 taaaggaaca agatttgttg aaagacactg ttctgatcgg actaggaacg aatggctcat
    33901 tctcggaagc tcagtttgat agtgtgatgg atgcaatcgg tgatcgaaaa gtatactggg
    33961 tcaatgtccg agtaccgacg aagcgctggc aaaatgacgt gaatgcgatg cttgaacaga
    34021 tgacgaaaaa atacgacaat atgacgctga ttgattggta cagctacagt aatgaccata
    34081 gtgactggtt ttatgatgat caagtccatc caaatcctga agggatgaca cattacattc
    34141 acttagtcag cgaagccttg ctaggaggaa gttcaagtac gaacagcagt gagacaaacg
    34201 tgacagaatg atagctacaa aaaacttcag gcaaaaacat ttgtttttgc ctgaagtttt
    34261 tttatattta ccgtgttttt aacattcata tagattcagg aagcaaaacg gttgagtgtg
    34321 tctgtaaata acgttctttt tgttttcggg tgagtttttt gaatgcagct tctgcttttg
    34381 ttgcttcgct tcttgaagaa aatacttctg catggatcat cttaacaggt cttctttttt
    34441 gaagacgagt atatttagca ccagtaccat tgttatgttc ctctaatcgt ctagaaagat
    34501 cggttgtata tccaccataa aatgtctgat ctttacagaa taatacgtaa aaataatgtt
    34561 catttgccat agagcatctc acgaatctct gcagtgtatt ctccatccat attgtaagtg
    34621 atcaatggtg gagcgacttt gaatccgtct aatttgcctt ctttgattcc ttcaactaac
    34681 aaaatatttg cctcttttcc aggttttggg taaacaaatt ggatcctttt aggtgcaatt
    34741 cgatagtctt ccatgacctg aatgatttct aagaaccgat caggacgatg gaccatagta
    34801 aagcgtccat ttgtttttag tagcttactt gaaatatcga tcacttcttc taacgtcgta
    34861 tgaatctcat gacgggcgat tgccaggtgt ttattagggt tcgttttatt tgttggcagc
    34921 cctttgaaat aaggtggatt acagacgact aagtcacaag aattatgtcg aacgacagat
    34981 aagctgtctt tcagatcgat ggtgtggaca gtcatttgtt catttaaatg gttaagcgcg
    35041 atactacgtc ttgccatgtc tgctagacga ggttgaagtt cgatagcgtc gatagccgcc
    35101 tgggttcgtt tacttaaaaa aagtccaact gcgccatttc ctgcacaaag gtcaacaatc
    35161 ttcccttttt taggtacacg ggaaaagtga gcaagcagca cagcatctaa agaaaaagaa
    35221 aacactttgg aactttggat aatcttgata tcttctgcgt ataattggtc gattcgttcg
    35281 tcttcgtgaa gcatggattc cctccaaaat gaaaaaaata gtcgtctgta ttatactatt
    35341 tttattaaaa agaaccaagg tggcttgtca aacctctatt ttttatgcat actatgacca
    35401 taagaaggga aaggaagaga ctatgtttta tcgatttatg cgcggggtag ttcgagtact
    35461 attagcctta gttaatggaa atgcccgcta tgagaataaa gaagttttac cgcaagatga
    35521 gaattacata ctagttgctc cccatcgtac ttggtgggac ccattatatc tggcggtagc
    35581 tgcagcaccc aaggaattca gctttatggc aaaagaagaa ctgttcaaaa atccagttct
    35641 gcgatttata ttaactcgat ccaatgcttt tccagttaaa agagaaaatc caggaccaag
    35701 cgtcattaaa acacctgtaa agattttgaa atcgacaaat ttgagtttga tcatgtttcc
    35761 cagtgggaca agacatgcta cagaacttaa aggcggaatg gcattgattt ccagaatgtc
    35821 taaagcgaaa atcgtgccag ctgtatatca aggtccgttg acacttgggg atctgttcaa
    35881 gcgtcagaga gtgacagtgc gtttcggcga accaatcgac ttatcagata tcaagaaaac
    35941 ggataaagaa ggtatggcgg agatcgaacg acgaacacaa gcagcttttg acaaattgga
    36001 caaagaagtg aaccctgatt tcaaatatga agtcaaaaaa aaataaaatg ataagtttat
    36061 gcgtgggcgg tcttttgaat ctactgtcct gttcagttca gactttttca atgaaaacga
    36121 gggagagacg tcaagtctgc cctctttttg cgttccaata tgtaaaatag aagacaggtg
    36181 tcatgtaatg gatcaaagtg ttataataat tagaaggaca ttttttgagg tgattgaatg
    36241 cgttttttac atacagccga ttggcatatc ggaaaaaaac tacaaggtta tgacttgatt
    36301 gaagaccagc gtcatgtact atcttctata ctagatatcg cgaaagaaga aaaagtagag
    36361 gcaatcgtga ttgccggaga cttatatgat aggagtgtcc cagctgttga tgccgtagaa
    36421 ttatacaatg aattgatggt cgattggaat ttaaaggaaa agatcccgat ttttgctata
    36481 tcaggaaatc acgatagcag tacgagactt tcagcgggta cgccatggtt cagttattcg
    36541 aactattttt tgcgaacaca gctagcagaa gcatttgaac cagtcgagtt taaaaacgta
    36601 caatttttct ttcttcctta ctttgaacca attgcagcga gattatattt tgaagatgac
    36661 aatatccgaa cgatcaagca agcggtagcc aaggtgatag ataaaatgca ggagctattt
    36721 gattcagaaa aaaaacaagt attgattagc catttttttg taatgggaag tgtaaaatct
    36781 gattcggaaa cgaaaatcga agtaggcggg ttggattcga tccctggtga actacttaca
    36841 ccgtttgatt atgtagcact aggacatttg cataatcaag ctgctttgag acaggataat
    36901 gctcgctata gcggttcccc attgaaattc tctttatctg agatcaacca aaaaaaaggc
    36961 gtatggatcg ttgacctgac aacaggcctg gagttgaatt ttagggagat cgaacccctg
    37021 catgatattg aagaaataac agcgagtttc aaagaattgc tggatcctgc cttttatcag
    37081 tccgtcaatc gggagaatta catctatatc tatctcactg atagaacgat catcccgaat
    37141 atgatgaacc agcttcggca aatatatcct cgtattttgg ggattgaacg ggcaaacggt
    37201 cgagcagaac aaacaaagcg caaacgaaaa gtactagaaa tgaaagatcc tcaaaaatta
    37261 gcagaagcat tttttagtga aatgacggaa gagcagctaa ctgcaaacca agaaaaatgg
    37321 ctcggagaaa cgttgcaaga aatcatggaa atggaatgat caagaatgaa accgaaaaaa
    37381 ttacaactta aaaattttgg tccttttatc aatgagaccg tagatttttc acgtttgaca
    37441 gaagcaccgc tttttttgat cagcgggaag acaggagctg gcaaaacgac gatttttgat
    37501 gggatcacct tcgcactttt tggtgaaact tccggaagac ttcgctctgg aaaagagatg
    37561 cgttctttgt ttgctacacc agacgaagaa acaagcgtga ctttttcttt tgaacatcag
    37621 cagatgagct atgaaatcga acgaaaacca gaacaagttt tagcaaaacg aaaaggaaac
    37681 ggaacaaaaa aacaggcagc caaagtaacg ttgaccattt ttgatgggaa aggaaaagaa
    37741 ataaaacaaa tcacaaaacg tacagaagca gatcagttga tcagagaact gatgaatctc
    37801 gatgcgaaac agttttctca aatcgtttta ctaccccaag gagaattccg taattttttg
    37861 gtttcatcaa gcagtgataa agagactgtt ttacgtcatt tattcggtac tcaatttttc
    37921 caacagttca atgagcgatt aaaagaaaaa gcaaaacaac agcaacaaaa attggatcat
    37981 ttagagcaag agctgcatct tctgcaaaaa aggtttgttt tagtcaaaga agaggaacca
    38041 gcagcagggt ttgaagccgt tttagcccaa tggacaaacc atcaggcaat actcgaaaaa
    38101 caaatcacgg aagaaaaaga agctctaacg gaacttcagg aaaaacaaaa gcaactagaa
    38161 aattcctact atcgttttga aagcttgaaa aaaagttatg aagaaaaaac gatattaata
    38221 aacaagcaaa aggaattagc tgctcaaaaa gacgaaatag aggaaaagaa acgatggatt
    38281 gggtattatg aatttacgca acgattagtc gaaccaatcc agcgcctaca agaaataaaa
    38341 gttgaaaaaa tagaactgtc acaaaaaagt agtgagcaaa caaagaaact agcgatgact
    38401 acgcagaatt atgaagagtg gcaaaaaaat gaagcaaacc gcgtacgccg acaaacaaaa
    38461 atcaaagaag ctgctcaaaa actgcaaaac aaacagatga tggtgccaat tgtcgaatcc
    38521 ttacgtagta aaaaacaaga cgcagaacaa ttaacaattt cattggataa agaagaattg
    38581 aatgccgaga agctgtatca aaatctcgct atctatcagg aaagaactcg aattcttcaa
    38641 gaaaatttac tggatcagga aaaactgcaa gaagatcgtc ttgcttttgg acagttaaaa
    38701 tatctagatg aaaagcagca agaaatagaa aaacaaaaag caaaagacac tgggtcgctc
    38761 gcacagataa gagaagaact caaaaaagca actgaagaaa taacaaaaaa tagtcagcta
    38821 ttgaaagaac agacagaaca agcagaacag ctgaaaagcc aatgggcaaa gcagcaaatt
    38881 gctcgtcttc agcttttttt gcgtccaggg gagccatgtc ttgtttgtgg atcaacggaa
    38941 caccccatac aacaattaaa acacgaacag ccaaataagg aagatatctt aaaagtagaa
    39001 caacaactgg cagaagctga gtctgctgta gaaagaactc agcaaatcat tgccaaaagt
    39061 gaagcacatg aaaataatct tcaacaacaa ttatcagatt tagaacaaaa aatcatggca
    39121 acgaatatac aattggctga acaagaagaa cacttgcaac aggctttgaa aaaggcattt
    39181 cctgagataa cagaacaaaa aacaaaaaca actcttgtag taatcgaaca atctttagat
    39241 agacgaaaaa gcaagatggc caatgcaaga atagagctgg aagaaataag acaacaagca
    39301 gaaaagctcg aacaacaatt agctgtctac aaacagcagc agacagaaaa aaataatcag
    39361 ctccagcaac tgcaaggaga aatcaaaagt ttagaacaac aattgcaaga tacggatagt
    39421 aactgtttag aagaggagat acgacgactt gaaaaacagt tagaagaaga ctcgaatcaa
    39481 ctagctgttg agcagaaagc aggagtgcaa ttgttgcaag atcttcatac gcttcggaca
    39541 cagcttgatc ttcaacatga acaaatagaa aaaatagatg aaaaaataga aaaggaacaa
    39601 gaaagaatag ctaaaaagtt agaaagccag tcgtattttt caacaattga agaaatctgt
    39661 gcatttgcac aacagcaagc acaatataaa gaaaaccaac gtacagttac ttcttatcat
    39721 gaagagcaat tagtgacagc tgaccggctg cgtcaattag aaaatatcca gctaactgag
    39781 acattcccag atacagaaga gatcagccaa cagttagaag aaataagacg tatgatcgaa
    39841 ataaagcaag aaaagatata cggtttacaa gaacaaaaaa ttagtaatca aaaaatctac
    39901 gatgaatgtc tttcgattta tcacacaagc cagacgaaat tagatgagct tacgcaatta
    39961 cagcagcttt cgcaaacatt caacggagaa aatcctaaga agactagttt ggaacggtat
    40021 gtgcttcagg tttatcttca agaagtgtta caagtagcaa atgatcatct ccaacgttta
    40081 acgaaaaatc gttatcaatt tgagctagcg gataccattg gcagttatcg agggaaaacc
    40141 ggattagaaa tcaatatcta tgatgatcag gcaggtatgt ctcgaagtgc gcataccctg
    40201 tctggcggtg agagttttat tgcagcactt tctttagcat tagctcttgc cgatgtgatc
    40261 cagacacaag ctggtggtgt agcaatcgat gcattgttta ttgatgaagg atttgggtca
    40321 ttagatgaag aagctttgga aatggcaatg gaagctttag aaacgatcga aagtgaagga
    40381 agaatgatcg gcatcattag tcatgtaaga gaattgaaag aacgagtatt acaacaaata
    40441 agaattgata cagaaggtag cggacaaagc aaaatacgct atcaacttgg ataaaaaggg
    40501 agattggtaa ctatgaaatg gagtattcca gaaaaagtca ttgcacgtgg acgtcaatac
    40561 ttgcaagaag gccgagtgtt atcggttgtc cctgaccaag aagaaaacgt gtggcatgca
    40621 gaagtcttgg gcagtgaatt gtatatggtt gatttggatg gtacagcaaa agagatagat
    40681 tactgccaat gtccctattg ggaggaacat aaatattgta agcatacagt tgctgtggaa
    40741 ctttatttac ggtcgaaaaa tctatctcgt atcatgaaaa aagaccagcc aatgctagaa
    40801 ccggtaaaag caacaagtga gggagaaatc attgcaaaag gtttttctcg tctaaaaaca
    40861 caaaacaatc ctcaagcggt gcaacctttg attattgaat accaagtaga aacgattgag
    40921 actaatcagt atcacccaga gctttctatt ttggcacttt acttgcgtat cggttcattg
    40981 gcatcaccga aaaaaacata tatcgtaaaa aacatctatg attttttaca agcttacaca
    41041 gaaaaagaaa cttacactgt taataaacag taccagtttc gtctagataa acgggcgttt
    41101 caaaaagaag atcaaaaaat actcacgcat cttgcaggaa gtgctcagac gcatcaatta
    41161 ttaggtacga acggcttaca agtcaaagga aaactagata aaagatatct gcttctccca
    41221 gtagaacaag cccgcttcct gttagagcag atgaatcaga ctacacgttt gcatctgcag
    41281 ataaatgaac aaaacgatca aggaatacaa ttttccgatc aaaacaaacc tttatcattc
    41341 aaagtggaaa aatttggaga agattatttg ctgcaagcat tgaatgattt tgattttttc
    41401 tttatgcatt atcagtgggg agttatccaa gggacatttt attctctgac gaaatatcaa
    41461 caaacggttt atcaaacttg gaaacaattg attcgccgat tggagaaacc agaagtagtg
    41521 ttcaagaaaa aagaattacc gtctttgttt aaagagatta tccctctgct gtcagagatc
    41581 ggcgacgtag ctattgcccc tgcagtagaa gaggaagtag cagatatccc cgtcgaattt
    41641 attttctttt tccgaaaagc aaaaggaaaa atccaagctc gagtcgattt cgtctatggc
    41701 gaagtcatct attcgactga cccgaagcat gaggtctcga cagaacaggc ttttcaggtg
    41761 ttacgtgaca cagtagaaga gaaacgagta cttgatctat tcagaatgta tcgctaccag
    41821 aaaaatgaaa ctggttatga acgtccattg ccaagcgggg aagaactata tgcctttttc
    41881 cgcacagaac tatctgtctt ccgtcagttt ggtgaagtca gacttggaag aaaattgcga
    41941 gagttgtatt tagatgcgca taaacatcaa ccgaaaatcg aagtaaccga gaccggttca
    42001 tggctcgata ttcgattcga tgtgacaggg atagaggagc aagaaatcga tcatgtcctg
    42061 caaagcttgc tgagaaatga tgctttttat acattagaaa atggtcaggt tctttctttt
    42121 gattcagaag aatttcaaca aacaagccaa gtgttgcaac aattacggga atcgatacgt
    42181 acagaagaag gaacgatcca tgtacctaaa aatcaaggat tgatcattca aaatcaattg
    42241 gaaaaaagca atgctacttt tagtgaatcg ttccaaacga tggtacaaga tctcattcat
    42301 ccagaaagat accaggcaca gcttcccaaa ggattgaatg caacgatgag agattatcaa
    42361 aagcaaggtt ttcgatggtt gaagatgtta ggtcattatc agtttggcgg tattttagcc
    42421 gatgaaatgg gacttgggaa aacactacag acaattgcct ttttactttc ggaaaaagaa
    42481 gaacggaaaa gcttctctgc tttgatcgta gcgcctgcga gcttaattta caactggcag
    42541 gcagaggtga gaaaattcgc cccttcttta agtatccaag taatcaatgg gaataaaaaa
    42601 gaacgtgaag aattactggc taaagacaca gatatccgag tgacttctta tgccagctta
    42661 agacaggatc tagctaatta tcagtcgcaa aaaattgatt atttgattct tgatgaggca
    42721 caaatggtca aaaatagcag tacgaaaact gcacaagcat tgagagaatt agctgtacct
    42781 cagcggtttg cactgagtgg gacaccaatc gagaataatt tagaagaatt gtggtcattg
    42841 tttgcaacga ttatgccagg atttttcccg actaaaacaa aattcaggga acttgccgca
    42901 gaagaaattg cgcaaatgat tcgaccgttt attttacggc gagataaaca aactgtgttg
    42961 aaagacttgc cggaaaaaac agagatgaat ctatattctg cacttacaga agaacaaaaa
    43021 actgtttatt tagcttactt gcgtcaaatg cgtgaagaaa tcacatcaat ggattctgaa
    43081 gcattcaaga aaaaccggat cgggatactt gctggtctga caagactgcg acaaatttgc
    43141 tgcgatcctc gtctgtttat tgaagattac caaggcggtt ccggcaagct cgaacaagta
    43201 aaagatctac tggtagctgc caaagagaac aaacgaagag ttttgctgtt ttctcaattt
    43261 acagggatgc tgactatctt gcaagaagag ttagccgagc tagggatctc gacattttat
    43321 ttaagaggca gtacgaaacc acaagatcgg ttaagtatgg tagacgcatt taatgctggc
    43381 gaaaaagatg tttttctgat ttccttaaag gctgggggaa ctggattgaa tctcacggga
    43441 gcagatacgg tcatcttgta tgacctttgg tggaatcctg ccattgaaga acaagctgct
    43501 ggacgtgcac atcggatcgg acaaaaaaat gtggtagaag tgtggcgtat gatctctgaa
    43561 ggaacgattg aggaacgcat ggattcactc cagcaagaaa aacgcgaact gttccaaaaa
    43621 gtgatccaag gtaatgaaga acagttgacg aaactgactg aagaagacat ccgcatgatt
    43681 ttaagtgtag gagaaatcga ggagtaagag actcgaaaaa gcgttcagta atgggtaaga
    43741 aagcctttct aaagaagcga ttgtttattt gatttaaaaa atagcaagga ggctgggatt
    43801 ccattttatc ccagcttcct tgctatcatt tttttatttc tatttttaaa ggttatacaa
    43861 agattgtctt cattgtctct aacagctgat caaaagaaga aatctcgata tcgcaaattg
    43921 gagtctgttc aattttgcgt tctcgatggt tgaaccaaag cgcttgccag ttagcacttt
    43981 ttgcacctag aacatcattg tcgtaattat cgccaacgta taaggtctct tctggaagca
    44041 tatgaaattc tttctcagct agctgaaaaa tctctttttc tggtttttgg aaacctgttg
    44101 cttgagaaat gagcatattg cgggaaggaa tccaattgtt caattgaagt tgcttcagtt
    44161 tttttgtctg atggtcagtc ggcccatttg tgataattcc taatgggaca ttcttttcct
    44221 tcaagaaatc gagggttttt ttgacctcct catgcaagca gatattatca agttcttcct
    44281 cgtaaatttt ttgaaataag aggccatctt cttccgtaat atgagggtag tctaaatctt
    44341 ttagtgattg gctgatccga tgtgctcgca tatattctag tgtccaatct cctgccatta
    44401 cttttgggaa gttttcatca ctatgatggc ggaaccggat atatagtgga tgcatatctg
    44461 catctgaaac aagcggaacg actcttttga ccgcgtttcg aaatggctgc tgctgatcat
    44521 acatcgtatc atcaacatca aacacaactg atttaatcaa aattttacac gcctttcggt
    44581 tttcattgat tttttcacaa atccttgact attgtaacag aaaaaattgt ctaaaaaagc
    44641 tgataaataa aggtttttct tactatattt ctatttcctt gacagaataa ggtttttata
    44701 tttcagtttt tttcactaaa aatcgtgaaa cacgtcaata agtttgatga acaaattaat
    44761 cattatggta gaagtcgatt gatttatttt aatgcaatct ctgtggaatt ttcgaaagat
    44821 ttattttttt aatacagtct caatataact attcaaacat aaaataacgt tatttgattt
    44881 atttgtttat agtaaatttt atttagtaat cattgcttta aaatactacg cttatataac
    44941 agagaaaata agcgttataa taaagaacag tattatttaa aatgacagaa aacattacta
    45001 ttaaagaaat aaaaaacata aaaaatctta agaaatcggt ttcatttagc ttttgagata
    45061 aaataatagt acagttttat acgctgtttc atatgtttag aaaatttgaa ttagatttga
    45121 aaactctaat ttttaaatga taaaaaaagc gagaatgctg gttttactgt atattaacct
    45181 tagataactt tcattttttt aaaaaaatta tgaaaaaaag gtagctattt aaaagttgat
    45241 tatgtataat agaaacagat gggagattat tatgatcttc tgttacaaaa aagtgattct
    45301 tatcacgaaa aggagtggtg cacgttgtta acattatata cttccccaag ttgtacttcc
    45361 tgcagaaagg ctcgtgcgtg gttgcaagaa catcagattc catttgttga acgtaatatt
    45421 ttttcagaac cattgaacag ttctgaatta aaagcgattt tacaaatgac agaagacgga
    45481 acagaagaaa tcatctcgac acgttccaaa gtattccaaa aactgaacat ggatttagat
    45541 gatctgccgt tacaagaatt attggaactt gtgcaaaaca atcctggatt gcttcgccgt
    45601 ccaatcatga ttgatgataa acgtctgcaa gttggtttta atgaagatga aattcgtcgg
    45661 ttcctaccgc gcgatgtacg acaattagaa ttacgtcaag cacaattgat ggctggatta
    45721 tagaaggata atacgaaact tggaaaagac tattacaaag atagtttttc caagtttttt
    45781 tgtttttaca aaaagtattc ttgattagat gtataaaaag gtagtagaag atgatgattg
    45841 cacgaaaaat agagagggaa aattagctga aaagtcggct atatagtatt cgtctgccaa
    45901 aaatgttata gttaaataag atagcttgaa aggttgaaaa atccgctggt tttattaaat
    45961 tcgcttaaca gcgagatttt ttttctttac atttaagctg atttcgctac aatgaatcta
    46021 tattaaacat tgtggcaaag tgaggtgtag caaaatggaa atggaacata ttaatgaaaa
    46081 tacgatccgt gtactaattg gtcatgaaga tttagaggaa cggggcgttt catttttgga
    46141 tttacttggc aatcacaaag aaatcgaaaa tttcttttat agtattttgg aagaagttga
    46201 tatcgaagag gagttccgag gaagcgaagc tgtcactttc caagttttgc caaaaggaga
    46261 cggattagaa ctgtttatca gtaaaaatct tccggatgaa gaaatttctc aatcagaaga
    46321 ttctactgtt gattcagaag atgtgagtga ctttcttcgt cagcagatta ttggcagcga
    46381 tccggaggaa gaattatctg gaagcgccct agaagaacag gaaagacaag caacttttgc
    46441 ttttgaagat tttgaacaag tgatccaact tgcaacagat gtcgaactag agtctgcttg
    46501 gactgatttg tatttattcg aagagcgcta ttacctagtc gttcacttct ggatggaaaa
    46561 tctcaaccaa gcggatgtag agaatcagat tgctcgaatt ttggagtttg ctgaaaagag
    46621 cgatcgtaca ccagaaacat tgaatgaata tggacaatgt ctgatggcgc gtaatgctat
    46681 cgagcgaacg aaattttatt ttgtataaat cagcattata gtaagagact gtgacaaaaa
    46741 ttgtgtcgca gtcttttttt cgtataaatg tgttcaaaag cttgcatctg cggtaacaag
    46801 cccaaccaaa cgaaaaatat tgggaactat ttttgtttag ctgttcttat tacttgatgc
    46861 agaatagctt tttcacgccc tcttttttcg tgtaaataaa ttcttagacg taaataagtt
    46921 cagagggaga aaaatgctgc ttgcaatgaa tgaatataat aagtgctgtt ttgctaaaca
    46981 tgcagaaaaa ggaaagaaat attattgtcg tggctgtcga aaaagagtga ttttgaaaaa
    47041 gggaaagaaa aaatgtgctc attttgccca tcaaaaatca gataactgtt cggtctttag
    47101 tgaaagagaa tcaaaagagc acttacagtt aaaggaatgc ttcatggatt ggctgggaca
    47161 gtcagtagaa ccagtatttt tggaagccta tttgccgagg ttgcggcaaa ggccggatat
    47221 tttgttggcg aatctagcaa tagaaatcca atgttctaga ctgtctcacc agagatttat
    47281 tgaaagaacc caaaattatt tgaataacag ttaccaagta tggtggatac ttggtaattc
    47341 ttttcttgga caatcgcaat tctcattgat agaaaaaagt tgctgctact ataatagaaa
    47401 aagaggagtg cattgctgga aagctgatct caagaaacaa aagctttatt tgtatcatca
    47461 tattacagag actgtatcag ggcatatttc cttttgcagt agctgctgga ctttttctag
    47521 tagggacctt aaagaaatat tcactagtaa cgaaattaaa atgaaccaaa tgaaaaaaat
    47581 agaaaaagtg agtgaagatg ggaagaaatg gctgacacgc caacttattc acaagcaaaa
    47641 gaatacagta tccatacaag aacaatgcta cttgcgtcat aaacatcttt tgcatctatc
    47701 atcgtggatc tatcaaaaaa gtcggttttt cttttattta caagaacagg tttttcttta
    47761 tcgaatgttg tacgaagaag ccttggaaaa ccagaaagtt cctgatttca actcatggct
    47821 ttgccaaata aaagaacata aaagggaatg gctttttcct atgattgatg aagagatggt
    47881 ctatcagcag ttttttaacg aatgcataca cttgtcttct ttgaaaaaat agtaagtata
    47941 aaaataggct aggaatcggt tttctatgta aaacaagatt tagttatgct aaaattgatg
    48001 gaagcagaca catgaatgag tagaaaagtt agatgctgtg acatgtggat gttagactat
    48061 gatctatttt gatagataga gctaagggaa gaaaaggagt ggatttcatg gaagtaaagc
    48121 agttgccaaa acgagaagaa ttgcctgaaa atttaacttg ggacttgacc aagatctttt
    48181 caagcgacca agagtttgat gagaaatatt tggaattatc agaagagtta aaacaatctg
    48241 aaaaacacaa aggaacactt gatcaaggcg cttctcaatt tttaaatgcg attgaattcg
    48301 tattgagggt ttatcgccaa actgaagtca tttatgtata tgcgcacctt aaaaacgatc
    48361 aagacactgg aaatacagat taccaagcgc tttatgcaag agcaagcagt ctgttttcga
    48421 aagttagtga agccgtttcc tggtttgaac cagaaatatt gcaattgtca gatgaccaga
    48481 tttggcaata tttcaaagaa gaaccaaaat tggaagtcta tcgccattat atccagcaaa
    48541 tagtagataa tcgagcccat gtcttatctg ctgagcagga atctcttctt gctggagcag
    48601 gtgaaatctt tgatgcttca agtgatacat ttgctgtttt gaataatgca gatctagttt
    48661 ttccaacgat tgaaggagaa aatggtgaaa tagtccaatt atctcatggc gtgtatggtc
    48721 agttgctaga aagcacggat cgaagggtgc gcgaagcagc atttaagggg ttgtacagtg
    48781 tttacgaaca atttagaaat acatttgctt ctactttagg cacacatata aaaggacata
    48841 attttaaagc gaaagtccgt aattacagct ctgccagaga agcgtctttg agcaataatc
    48901 atattcctga aagtgtatac gatactttgg tagacgtggt aaacaagcat ttgcctttgt
    48961 tacatcgata catggaatta cggaaacgtt tattagaagt ggaaaaactg cacatgtatg
    49021 atctttatac accggtctta ggggaagctc caattacctt tacgtacgaa gaagcaaaag
    49081 aaaaagcttt agaagcactg aaaccaatgg gtgaagaata catggccatc gtagaaaaag
    49141 cattctctga acgttggatc gatgttgtcg aaaataaagg gaaacgaagc ggtgcttatt
    49201 cttcgggaag ctatgacaca aatccatata ttttattgaa ttggcatgat acgctggatc
    49261 agctatttac gcttgtccac gaaatgggac atagtgttca tagttatttc actcgttcga
    49321 accagcctta tgtgtacggc gactactcca tctttttagc agaaattgct tcgacaacga
    49381 atgaaaatat cctaacggag tatttattgg aaacagaaaa agaccctcgt gtacgggctt
    49441 atgtactcaa ccattacttg gatgggttta aaggaacagt tttccgccag acacaattcg
    49501 ctgaattcga gcatttcatg catacggaag atgaaaaagg tgtgccattg actagtgaat
    49561 acctaagtga tagttatggt aaattgaatg caaaatatta tggtccagca gtcgaagaag
    49621 acccagaaat taaattcgaa tggtcacgga taccgcattt ttattataat tactatgttt
    49681 tccaatactc gactggcttt tctgctgctt cagcactggc gaagaaaata ctaaaccagg
    49741 aaccagaggc actagagaac tacttggctt acctaaaagc aggtaacagc gattatcctg
    49801 tggaagtaat gaaaaaagca ggagttgata tgacacaggc tgcatatatt gaagatgcaa
    49861 tgtcgatgtt tgaacaacgt ctaaatgaat tagaagaatt gatcgatcgt ttataaggct
    49921 ttctattttt gattttctcg aatagaaacg aaaaaaatac gtcggctctc ttgtaaggga
    49981 gtcgacgtat tttttt
//
LOCUS       DASHHB010000014        48756 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.014, whole genome shotgun sequence.
ACCESSION   DASHHB010000014 DASHHB010000000
VERSION     DASHHB010000014.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.014"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            23..646
                     /locus_tag="U2K71_000910"
     CDS             23..646
                     /locus_tag="U2K71_000910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315015.1"
                     /translation="MFVIWEVVKSRVQEMNEHFSVSFAENPSKYFRRLVFLLILSLLL
                     TVAMFLLPEKIREEQVNSLVYSSIPTSKLKPFDYSKADQKIKQSRAISVMFSKPNGKT
                     YQDMLDVFNDPELMEELNRPIFYYPIVYDVHELEQKYNIRTDEVTFIFFDGGKEANRI
                     TAGKGGITDFDKELIPELNRLPLANIKQLEEELDRTGVTSQNSEIAN"
     gene            1096..2145
                     /locus_tag="U2K71_000911"
     CDS             1096..2145
                     /locus_tag="U2K71_000911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM7315016.1"
                     /translation="MKNKRLITVVVIILVYLAGAFIYGKASGEKESTSTNQTKKTTVV
                     GVLQYVSHPALDEIYRGIKDGLKEEGLKEGENLEIKFQNGQADQSKLATMSQQLVQSD
                     PDALIGIATPAAQSLANTTSSIPVVLGAVTDPVGAGLVASLEKPGGNVTGVSDQPPVT
                     SQIKLASDLLPNAKKVGILYASSEDNSKYQVAQAEEAAKKEGLESVKYAVPSTNEIAQ
                     TVQVMSRQVDFIYVPLDNTIANAMQTVVKEANKANIPVIPSVDTMVEQGGLATIGINQ
                     YQLGLQSGKMAAELASGKEKPETTPVYTFDQGDTVINQSQADRLGIIVPQSMKEKAKI
                     ITDDSQQETSKEDDK"
     gene            2142..3023
                     /locus_tag="U2K71_000912"
     CDS             2142..3023
                     /locus_tag="U2K71_000912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315017.1"
                     /translation="MIVSAISQGMLWAILGLGIFMTYRILDFPDMTTEGSFPLGAAVC
                     VTAITHGVAPIVATLLGVGAGMIAGLVTGLLYTKGKIPVILAGILVMSGLNSVILFVM
                     RTPNLSLLNHPILQDSFHRFGLPDYFDTVLLGFISLGILIAVLLFFFNTDLGQGYIAT
                     GDNETMARSLGIKTDRMKILGLTLSNGVIALSGALIAQNDGYADVNKGIGVIVIGLAS
                     IIIGEVIFQELTLAERLIAIVVGSIIYQLLILVVIKLGFDTTYLKLFSAIILAICLMI
                     PQLKKALNLKTGFEKEA"
     gene            3026..3796
                     /locus_tag="U2K71_000913"
     CDS             3026..3796
                     /locus_tag="U2K71_000913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7315018.1"
                     /translation="MTDILTIKNGRKVVNNGMNEEKVLLDEINLTIREGDFITVLGGN
                     GAGKSTLFNTIAGTLQLSQGSISFKDQLITKDSEEKRARFLSRVFQDPKMGTAPRMTV
                     AENLLLAQKRGQKRKFRLRNLKNQKEDFFRLCQEVGNGLEQHLDTPAGELSGGQRQAL
                     SLLMATITAPELLLLDEHTAALDPKTAKALMRLTNQRINEQKLTCLMITHRMEDALKY
                     GNRLIVLQKGKIVQDISEKEKQHLTMGDLLQFFEEVVD"
     gene            4075..4929
                     /gene="aroE"
                     /locus_tag="U2K71_000914"
     CDS             4075..4929
                     /gene="aroE"
                     /locus_tag="U2K71_000914"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HEM7315019.1"
                     /translation="MISGQTKLAGLFADPASHSLSPLMHNTAFEANNIDAVYLAFQVN
                     PMNLKQAVESIRTFDMLGVNLSMPNKTAVIPYLDEVSQEAQLIGAVNTIVHEKGRLIG
                     YNTDGAGFMQSVQEAGISIKKKKMTLLGAGGAAKAVTVQAALDGAEEIVVYKRNNQTF
                     AQVKETFEQLSYRTSCKIKVRDFADTHQLRKDLKESCLLVNATDIGMGKKINQSPLLD
                     ISMMHENLAVFDLIYAPRETRLLKEAKKVGAKGYNGLGMLIYQGAIAFELWTKEKMPI
                     HVIEKLFD"
     gene            5107..6270
                     /locus_tag="U2K71_000915"
     CDS             5107..6270
                     /locus_tag="U2K71_000915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM7315020.1"
                     /translation="MSRMDRYKDIHKKAKPLKRDTGSIFARRERKRTRMENEEDATRI
                     YEREQHSFEHLNQYEQQIYRDAPRAEEQQKNKKFFNRQKKNKPLKPRRKRSWKKIILS
                     ILLVLILYSGISFFIGKYVAEHDTSLEAATSETFHGQKATNGAHNILILGSDTRGEDA
                     GRADTIMVLQLDGPSHKPKLISFMRDSFVTIPGVGQNKINSAYAYGGADLVRQTLVEN
                     FGIDCQYYAKVDFKSFEKVIDALFMNGVKIDAEKDLNLDGVDIKKGVQKMDGHVLLQY
                     ARFRMDEQGDFGRVRRQQQVMNAIFSQLKNPLNLIRAPYAAGKALGYTSTDVTAWFLT
                     KNLFSIARGAGGIDRISVPVDGSWNYGQSDYAGSILEIDNQMNQSAITDFLNK"
     gene            6387..6797
                     /locus_tag="U2K71_000916"
     CDS             6387..6797
                     /locus_tag="U2K71_000916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM7315021.1"
                     /translation="MDIYLNFKNESREAIAFYEKVFQTEASGITTYGEMPGIEQMNLP
                     EEAKNLITNARLNIYDTLVMFADTPEGIGPELVVGNNVSLVIQTRNEEETKRLFESLA
                     EGGKIEMPLDAVPWAQLYGMVTDRFGINWQINQE"
     gene            6971..9199
                     /gene="topB"
                     /locus_tag="U2K71_000917"
     CDS             6971..9199
                     /gene="topB"
                     /locus_tag="U2K71_000917"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEM7315022.1"
                     /translation="MKTVILAEKPSQAKAYAETFQQQKRHEGYYEISDPLFAGEVTIT
                     YGFGHLVDMVAPGAYEERWAKWSLENLPIFPETFRYEVPMDKRAQFSIVKRELQSADT
                     IIIATDGDREGEAIAWSIIRQANAFSKEKNYLRLWINSLEKEAIYEGFKNLQPGKNYF
                     PKYKEAQARQNADWLIGMNGSPLYSLLLQRKGIDGSFSLGRVQTPTLYMIYQLQEEIK
                     HFKKETYFEGEGLITTNKGQFSGKIVPKKAFKTQEELTKEIEKLGAHLGKQNGRILEV
                     TKKEKRLHSPRLFSLSSLQTKMNQLMKASAKATLDAAQGLYESKFLSYPRTDSFYITE
                     NEHQYLVKHLQNYKKFLGIEEVQTTEIKAKKRYVDAKKVQEHHAIIPTKTIPTPARFA
                     KLSKLQQAIYLQVLRTTVAMFAADYIYEETTVMTGVQQLKLKSIGKIPLQQGWQEILR
                     IKSKKKEAQTLPPIVENEEAEVELKSMEKETQPPKPYTEGTLITAMKTAGKTLDNDEA
                     QEILKEVEGIGTEATRANIIENLKQKHYIEVNKNEITVTPKGITLCKAVANEPLLTSA
                     EMTARWEGYLRKIGKQEGTPSVFLENIKKFILHLLAEVPNQIDKVDFSDEISGTRQIK
                     AMEKKNDQLGRCPKCKKGIVMLYPKIATCLNPECDFKLWPTVAKKKLTKTNLRDLLSK
                     GKTSKVVKGFTGKKGKFDAVLVLKEDMTIGFSFPWIENSGTKAVDGNKETSRKNNENE
                     VE"
     gene            9370..9654
                     /locus_tag="U2K71_000918"
     CDS             9370..9654
                     /locus_tag="U2K71_000918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315023.1"
                     /translation="MGAITIVAVYQAFLKYPDYGTKLLSLTHNQTAYSPTLILSTLTA
                     GAKSATYFFHKTTSMLKRKKQRKQLLVVVAAMIIIYFLPVITQVFHQYFG"
     gene            9814..10050
                     /locus_tag="U2K71_000919"
     CDS             9814..10050
                     /locus_tag="U2K71_000919"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM7315024.1"
                     /translation="MKKMKKYKIRKSGNSDVTTIPPEVKEFMGVQTGDAISYVFQSDG
                     SVRMIKAQEEPDIDSLVDSIMNQYEDALKDLVDL"
     gene            10047..10445
                     /locus_tag="U2K71_000920"
     CDS             10047..10445
                     /locus_tag="U2K71_000920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system death-on-curing
                     family toxin"
                     /protein_id="HEM7315025.1"
                     /translation="MRYLSTKEIIKINAKVILRHSPGEMIGVKDANALDMAVKQPSQA
                     VFNQELYPEIYEKAAILAINLAKKHPFHNGNKRTALVAMLLFLQLNNCPVAFSRQEAV
                     DFIIMITTSSLDFDSLKSKITSYLRQTAIK"
     gene            complement(11112..12056)
                     /locus_tag="U2K71_000921"
     CDS             complement(11112..12056)
                     /locus_tag="U2K71_000921"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HEM7315026.1"
                     /translation="MKKTSRKVVIVGTGFVGTSIAYAMINQGISNELVLIDVNQEKAE
                     GEALDLLDGMAWGDENVAVWSGGYEECKDADIVVITAGINQKPGQSRLDLVKTNASIM
                     RQIVKEIMGSGFDGIIVVASNPVDILTYIAWNESGLPTSRVIGTGTTLDTTRFRKEIA
                     LKLKVDPRSVHGYILGEHGDSEVAAWSHTTVGGKPVFEIVEKDHRIAQDELDVIADKV
                     RNAAYEIIDRKKATYYGIGMSTARIVKAILNNEQAVLPVSAYLTGEYDEKDIFTGVPS
                     IVDENGVREVVELSINEEEKAMFSKSTSALREVLNTVL"
     gene            complement(12352..13296)
                     /locus_tag="U2K71_000922"
     CDS             complement(12352..13296)
                     /locus_tag="U2K71_000922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315027.1"
                     /translation="MRNEESIETLQREQGMNINDLNLAYHLQTPPKIHRGDLFQQGNI
                     HISKHRRYAEVPEHTHDFVEINYMYSGTCLQIINGERIEMEESDVVIIDKEATQRIEY
                     TGQEDILINILLKEETLSTEILNHLASTNNLLSSFLINASKEKGEHTQFIHFRGGKTS
                     RLTHLIENMLLIYFDTYSHKIRSLNLYTSLLLIELTHLLEQQSFSSADGCAENEMLRV
                     LDYIDKHFTSCTLKETAAHFGYNPVYLSNKLKMYFDASFQELVLKKKFDFALELIQET
                     SFSLEEIANRIGYKQTTSLFKLFKKYSTCTPNEWRNKH"
     gene            13517..14731
                     /locus_tag="U2K71_000923"
     CDS             13517..14731
                     /locus_tag="U2K71_000923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7315028.1"
                     /translation="MLMNKKMMKFSLLSVSLILTSAGAVTSAIPSMSATYATRSLSAV
                     ELLTTAPSFMVTIFVLISSFIARKIGQKQTVIIGLVIASICGILPLFSQSYSIVLASR
                     AGLGIGFGLINSLAVSMIAAFYHGDERASMIGFQSAFQGFGAALMTFVAGQLVQFGWQ
                     YTFLVYAISLPILVLFVLFVPTPETTELQSHGTAPTRQVIKTNPKMISYGIFLLLVIV
                     VYNAVSIKLATVLTTYSLGNETNAANILTIMQLASMAAGVCFGWLQTKAHRYMLTLSL
                     LLMGIGFVLIATVINLYVIGFGAILTGISFSLFIPYLFNQVSIQAPKYAESFNTSILL
                     VGANLGTFIAPYGLLFLSFASLGSKTLAVFVNGSILLLISALISLLVVHRTNRKTVIR
                     VDEEIKEQLTTK"
     gene            14753..16768
                     /locus_tag="U2K71_000924"
     CDS             14753..16768
                     /locus_tag="U2K71_000924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 78 glycoside hydrolase catalytic domain"
                     /protein_id="HEM7315029.1"
                     /translation="MTNEKHKDRWLWYPGDFEIRHGLLQNFQREERGFDWPAYWYMDD
                     CHRNVKFKRYYFLDQPSMFKVTIQGVGYVEINGQKHPCGKWLTCPAGKAKIRIFVGHT
                     SGLPAMFIEGDEVKSDIGWTASNFIEEYPAGWSPLYVDIAKDPNQIYYQKECVHPVTE
                     TEIGGGILFDFGRAVNGCVELISLDDHIESITLCYGESDVEAMDIDYCYYKQIGMTRK
                     MKARKRAFRYLFIPEVSTGMVQLQVFHEFLSLSKQAAFSSDSSLLNQIWSVSEEAFKL
                     CSDLFFIDGIKRDRWIWSGDAYQSFMINQYLFFDEEINKRTILALRGQNEIKQHMNTI
                     VDYSILWLIGIENHYMMTKDYEFLRIVYPKMQSLMRYLMEQTNELGFIYGRAKDWIFI
                     DWSEMDKEGTLAAEQVLLLKAYHSMITCGDILAETYGENENYHTHLYQEKYQLLKQNL
                     FDYFWDEELGAFIDCYESGRRNVTRHANIFAVLFDFANKDQQQSILKNVLLNDTITQI
                     TTPYFKFYEQDALCKLGQTNIVYQTILDYWGGMLSHDAVTFWEEYDPTQTGNEKYSMY
                     GDPYGKSLCHAWGASPIYLLGRYFIGLYPTKPGYEEFMIQPNLDSFETLNCTLPIKDG
                     KISLEKTKDKIVITSDRTGGTVKMNGKSYPLLAKQPLVLTSYSKINA"
     gene            complement(16851..18224)
                     /locus_tag="U2K71_000925"
     CDS             complement(16851..18224)
                     /locus_tag="U2K71_000925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEM7315030.1"
                     /translation="MDKLLNLFVSIVSQPSILVSLIALLGLVLQKKKFSEVIQGTIKT
                     FVGFLVLIGGAGLISNSLTPFASMFQTALHTQGVVPSNEAVVAIALQQYGTPTALIML
                     VGMIVNILLARFTRYKFIFLTGQAMLYVSCLTAVILVSAGFTANLWMILLGGVFEGTL
                     LTITPALCQPFMKNITGNNQVALGHTGNIGYASSGWIGKFFGNKDRSTEDIKIPKSFS
                     FLRDSTVSIMLLMSIVYVALALLAGPGFVERELSNGDNAVIFALIQAGTFTAGFVIVL
                     QGVRMVLAEIVPAFQGIAKKLVPDSKPALDVPIVFPYAPNAVLIGFFVSFFVGTLSMF
                     AMIGLQTTVIIPGVVGHFFCGAASGVFGNSTGGRRGAVLGSAFNSLLISWLPLFILPV
                     LGDLKIASSTFADTDYLVPGLVLGKLGEYGQIALTVGIIAFFLLVVLSSFLFAGKRTN
                     IVSKEQN"
     gene            complement(18250..18534)
                     /locus_tag="U2K71_000926"
     CDS             complement(18250..18534)
                     /locus_tag="U2K71_000926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7315031.1"
                     /translation="MKIFAVCQSGLGTSFMVEMNIKQALADLGADVDEFEVSHTDVGS
                     VSSDMADYFFIEHSLKDTLTSIPDEKLVPLQSIIDSEEVKEKVTKILSKE"
     gene            complement(18536..20551)
                     /locus_tag="U2K71_000927"
     CDS             complement(18536..20551)
                     /locus_tag="U2K71_000927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM7315032.1"
                     /translation="MNYKISYLLKTPREISLEKAAEIIEVNTSEAIKQLTPFAEYVDD
                     TRIRIKKFRAIDWAEKFLDITRFEAVYTEQERRDLLYLMVYSTATSVSVFHFQDFLQV
                     SKGTVLSDIKKIRNELEVEDCQLSYTRKTGFRLLGSEAILRREAKNRTARLMQSLAGR
                     FGLTYLTATLSIDYYAKVRDLLDKTISLSDLRFAPNRMDELAYYLSLTTIRIGKTSME
                     LVKDAELLASLAVTDISRQVLTGLGYSEHQNSEIIFFSLCLASIVEGDVQEPALDFLL
                     ECSSEVIHRMEFLMAVEFASFRELLMNVFYHLVPAYFRISYSFYLPNVMIDQIKHQYA
                     SIYEMTRKALLPLERRIGKSIPEEEIGFFTILFGGEIRKADAEERNRKIRAVIVCPSG
                     ISSSLILKSELQQLFPMIFFTETNSADRLHEVDETSYDIIFSTVPLTLTDKKKSLYVL
                     HPIMTDLEKNQLINQVQRDWLIPGFSMPSAQELLSALLPYIELKEGVDEEKLYQVLNR
                     KMNKLLEKKEDQRPMLSELLTEEMIQLSDQPKNWQEAITFAAQPLLSENKIEESYIKA
                     MIERVEQYGAFIHIGDHIALPHARPEDGVNEVGMSLLKLQQPVDLLDNPAHPISLFIC
                     LAAIDNEAHLRALANLTKLLSNKQNLQDLLHASTKEEILAIIRKGDE"
     gene            complement(20730..21035)
                     /locus_tag="U2K71_000928"
     CDS             complement(20730..21035)
                     /locus_tag="U2K71_000928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA processing protein"
                     /protein_id="HEM7315033.1"
                     /translation="MTNYDGEKHKRLRGSMAPKTEEEVALAGTNSGETEDKEESLAGT
                     NSGETENKETALSGSNSVEEETKPNEPNHFEKDIEKAVEDVKEKFEELTSKNKHDHH"
     gene            complement(21124..22155)
                     /locus_tag="U2K71_000929"
     CDS             complement(21124..22155)
                     /locus_tag="U2K71_000929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM7315034.1"
                     /translation="MLTIAYLGNGKSTNRYHLPFSRKLTNKINIKKIFSPSEPVWKTF
                     EDIEYTDQLSDLWDDPEIQLVVITTPSQFHYEYGKMALEHGKNVLVEKPFAETSKEAE
                     ELFALAKEKGLFIQCYQNRRFDSDFLTVQKVIESGKLGDILEVEMHYDYFRPEVPENT
                     HEFSTANSYLYGHACHTVDQVLSYFGEPDDISYDVRQLLGKGRMNDYFDLDFFYGNMK
                     ISIKSSYFRIKERPSFVVYGKKGMFVKQTKDRQEEHLKMFYMPDHEDFGIDLPEHYGV
                     LTYIDEQGSYHEEKVISEVGDYSRVYEGIYETLVNGAEKVIKDEETILQMKILEEGIK
                     SIQESEGKI"
     gene            22362..23237
                     /locus_tag="U2K71_000930"
     CDS             22362..23237
                     /locus_tag="U2K71_000930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM7315035.1"
                     /translation="MLIEEKLTKQELFTTTEKRIVDYIRRNIEAAVYMTIEELAKATY
                     TSHSAIIRLCKKMGFSGFKEFRFELGREVHQLIAQFNQTIDPNFPFSSEDSTQQIAKK
                     MADLSIQSIKKAQLQIEHEDLDQIGKVLNKAQRIFLFAKGDSQITARKFQNKMVKLNK
                     FLIMAEEYSDSSWNAANLTSEDCAIFISYSGRIHHYERIMTYLKHVEAPTLLITGNQH
                     SEMAKQADMCLVISQEEYDFAKVATFSSQIAFDYVLNTLFSVIYSQNFEENIRNLKNK
                     QELMQKGLLKEYRKT"
     gene            complement(23389..24231)
                     /locus_tag="U2K71_000931"
     CDS             complement(23389..24231)
                     /locus_tag="U2K71_000931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM7315036.1"
                     /translation="MKPKKRKLLDLLMLFLLLSGIGILAYPFVSDALNNYLDQQIISH
                     YQQQAVKENEEVMAKIQENMTKKNQQLAKEGGNPGADPFTKKKEPAKKDRTYFEKHTI
                     GILTIPEINVHLPIFDETNTVLLEKGASLLEGTSYPTGGENTHAVISSHRGLPQAKLF
                     TDLPKLTLGDSFYIEINGRTLAYQVDQIKTVEPTDTKDLRIEKGQDFVTLLTCTPYMV
                     NSHRLLVRGHRVPYHAKEVQKEVQKVSENKRFFLYGLLAACILIFLLLIYLFRQSFKI
                     RNRS"
     gene            complement(24323..26200)
                     /locus_tag="U2K71_000932"
     CDS             complement(24323..26200)
                     /locus_tag="U2K71_000932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM7315037.1"
                     /translation="MKNHKKINVMLGVLFLILPLLTNSFGAKKVFAEETAAQVILHKK
                     KMTDLPDPLIQNSGKEMSEFDQYQGLADISFSVYNVTQEFYAQRDKGASVDAAKQAVQ
                     SLTPGTPVASGTTDADGNVTLSLPKKQNGKDAVYTIKEEPKDGVSAAANMVLAFPVYE
                     MIKQADGSYKYGTEELDTIHLYPKNTVGNDGTLKVTKIGTAENEALNGAEFIISKEEG
                     TPSVKKYIQSVTDGLYTWTTDQTKAKHFITGHSYDIGNNDFAEASIEKGQLIVNHLEV
                     GKYNLEEVKAPDNAEMIEKQTITPFEILANSQTPVEKTIKNDTSKVDKTTPQLNGKDV
                     AIGEKIQYEISVNIPLGIADKEGTQNKYTTFKLIDTHDAALTFDNDSSGTYAYALYDG
                     NKEIDPVNYSVTEQTDGFTVSVDPNYIPSLTPGGTLKFVYYMHLNEKADPTKGFSNQA
                     NVDNGHTNDQTPPSVDVVTGGKRFVKVDGDVTSDQTLAGAEFVVRDQDSDTAKYLSID
                     PSTKAVSWVSAKESATVFTTTSNGLIDVTGLKYGTYYLEETKAPEKYVPLTNRVAFTI
                     DEQSYVTAGQLISPEKIPNKHKGTLPSTGGKGIYVYIGAGVVLLLIAGLYFARRKHSQ
                     I"
     gene            complement(26197..27618)
                     /locus_tag="U2K71_000933"
     CDS             complement(26197..27618)
                     /locus_tag="U2K71_000933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM7315038.1"
                     /translation="MKKLGWLSMCLFLLLFKPAFTQVATETETEMVQITLHKLLFPNG
                     QLPKNHPNDGQEKALLQTYRGLNGVTFQVYDVTDSFYHLREKGKTVEEAQAEIAKNGA
                     SSGMFTAEATTTTLNNEDGIASFSLAAKDQEKRDKAYLFIESKVPEVVKEKAENMVVV
                     LPVHGQNNQKLSTIHLYPKNEENDYPDPPFEKVLEEPRNDFTIGEKITYSLHTTIPVN
                     ILDYQKFELSDSADEALTFLPNSLTISSNGEKLTEGFVIHKKPHGFDVLFSIPSLEKY
                     AGKKLTISYQMQLSSTAQANKEINNNGTLDFGFGVSTKKVSVYTGSKQFVKIETNKPD
                     KRLAGAVFLIKNKAGNYLQQTANGYKWTKNESDALHLISDKNGAFSISGLKTGSYRLK
                     EIEAPSGYILSETEIPFTISTFLSEDKEADSILKVVNKKENSRPFLPKTNETKNTLLG
                     VVGMVFASFAIWLFIKKRTGVKK"
     gene            complement(27621..31010)
                     /locus_tag="U2K71_000934"
     CDS             complement(27621..31010)
                     /locus_tag="U2K71_000934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HEM7315039.1"
                     /translation="MTTTGKKLKVIFMLIILSLSNFVPLSAIADTTDDPTVLETISAE
                     VISDQSGKKALNIKLNANNTSAEKIEKEIGLVENYLSDVERKEGDGYAYQVNSGKITL
                     EISSNTKQTIDLSFPIDPALYHSQANKLIVDNKEYDIIDETENKKDTDVSVPKPDEIE
                     EESSKENENSVSPFTLPTLSLPAVSVPSNQTIPTEYTTDDQGTYPKASWQPTGNTNVL
                     DHQGNKNGTNQWDGINSWNGDPNDRTHSYIEYGGTGNQADYAIRKYAKETSTPGLFDV
                     YLNARGNVQKDITPLDLVLVVDWSGSMNDNNRIGEVKIGVDRFVDTLADSGITDKINM
                     GYVGYSSEGYSYSNGAVQMGSFDSVKNQVKSITPSRTNGGTFTQKALRDAGSMLSVPN
                     GHKKVIVLLTDGVPTFSYKVQRVHAQSSSNYYGTQFSNTQDRPGNTSLISRIYDAPDQ
                     NNLSRRIDSTFIATIGEAMALKERGIEIHGLGIQLQSDPAAGLSKAEVESRMRQMVSS
                     DEKGDLYYESADHATDISEYLAKKAVQISATVSNGQINDPIAEPFIYQPGTLSVKSVG
                     TSPTTVTPSISIEGNTIKSNQIYLGKDQEIQIHYQVRIQTENEDFHPNFWYQMNGRTT
                     FQPNIDTNELAEFGIPSAKAPGVSLHIKKLWEEFDNNLADRPDQVTFEIQREHTTNAA
                     AWKNGYIRIIKPAKDTTNTWERADIDKLSANSGESYQEILSLPQYNNQGQAFSYQTIK
                     ELPVPGYDSQQIDAMTWKNTKQFTPLNLKITKNSSTGEKDLIGAVFKLTGDSIDTLLT
                     DHGDGTYSLPENVKLQKEMTYTLTETKAPEGHGLSKKTTWEIKIASDGTVTIDGKTVT
                     TSDDTIQLTIENPFVEVPVAVRKYAMQGTDKEINLKGAAFSLQKKEANGTYQPIDSQT
                     TNEKGLASFDSLTPGKYRVVETAGPAGYDTSPGNYEFQIDKYGKIIYTGKNTEMTNNV
                     WTLTHQNRLKAFDLTVHKKEDNGQTLKGAKFRLQGPEMDLESPKDGQETDTFLFENLK
                     PGTYTLTETFTPEGYQGLKEPVTIVIHEDGSIQVDGQDHESVLSPGAKNNQISLDITN
                     QAKVPLPETGGIGRLGIYLVGMIGCAFSIWYLFLKKERGGS"
     gene            31267..32658
                     /locus_tag="U2K71_000935"
     CDS             31267..32658
                     /locus_tag="U2K71_000935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315040.1"
                     /translation="MEKIIRILSISDKWMTKKELAKRIGSSESTFVRYIEEIKIRWGT
                     DVTIQSSQKLGYRLEHLNVPAYLNVLMEMSQSSTNSRLLNEIIVNPGKTIEYYCEKIS
                     ISRSSFTRKLKQCNQILESYLLKVIVDQGFQLTSLKSELQLRIFVTFFFLSYYGRRTL
                     PFPIEKKKIKEIMVKNNCQMNLFQEESPYEQSFFIMYFVVHLMRESQGTPFEWKAGDS
                     YVQLINIDKKDFLILKNLLGKLKYSSYIKVVNYFVNTIFSEFSEKEKNEISEQIRKNM
                     SYCSFSSADCKEKNEEYVVQLLTQIYFFSRIFPYDTTHLTIRATYFAQQMQHRQIRLY
                     AGFRAELQFYSTLLNQNLIYYLSSYIFWMLIGNPEIHHYTSDKKILIISDLSLRHSQY
                     IEEYISDILAVHKIHSETTAITEDQLSKYNLLEYDLIVTNQPILNAHVPHILIDDSVS
                     FANEEELIRLFEL"
     gene            complement(32731..33378)
                     /locus_tag="U2K71_000936"
     CDS             complement(32731..33378)
                     /locus_tag="U2K71_000936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEM7315041.1"
                     /translation="MTMKGYPRIIRDKGEMWMPKETFFNLSNAKKQRIDDLLLEIFCS
                     QHISQVKVSEIVDKMGMSRGAFYKYFQDLEDAYLYTIKSSSHIVHQDILKFISENKQH
                     FFLGIEKYLEWCCDLDHESIYWQRLYLLTQRNDQQTTKRRELSADSPMNEKWLELLRI
                     NHFSIQSSEEAVSFLYFSMTLVMNTLTDLIANNWTKEELLKDFRYKSRWIAEGIQ"
     gene            complement(33386..34072)
                     /locus_tag="U2K71_000937"
     CDS             complement(33386..34072)
                     /locus_tag="U2K71_000937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002366355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315042.1"
                     /translation="MKKILTMKKVTKTFGSGHTKVSALKAIDFYVKEGEFVSIIGPSG
                     SGKSTFLTISGGLQTPTSGEIVINGHSLTELPEKKRADLRFKEIGFILQSSNLIPFLT
                     VKEQFELVDQVTSKKEDSTKIDSLLQSLDIQSLKNSYPKDLSGGERQRVAIARALYNE
                     PSLILADEPTASLDTDHAYEVVKLLVKEAHEKQKATIMVTHDVRMIQWSDRVYRMEDG
                     YLTEEEKKKE"
     gene            complement(34078..35151)
                     /locus_tag="U2K71_000938"
     CDS             complement(34078..35151)
                     /locus_tag="U2K71_000938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315043.1"
                     /translation="MFLAIKEIRYSKLRYGLIVGIMFLIAYVVFMLSGLANGLSEEFK
                     KAIDDWDAQEIVLSEDANQVFAASQLTRGDLKYIEGGEKAPIGLYSGAIKGKNKENVT
                     VFGTTKEAFLLPKLTKGHEFKTKNEIIISQNLADSGYKIGDTIKIGSYDDPLTIVGIF
                     PETYYTVSPVIYASLDTWTALKYGQQPFASEEEQPINGIVIKDSAKISKEKASKGLQL
                     LTIGTFIESIPGYSAQNMTLNAMIYFLFLVVAAVVGIFMYVMTLQKTAIFGVMKAQGI
                     KNFFIAKSLVAQSFIVGVVGVLLALLFAYLTSLILPSAMPFAVFWSQWLLYSGILIIV
                     AMLGGLFSIRTITKVDPITAIGG"
     gene            35373..36047
                     /locus_tag="U2K71_000939"
     CDS             35373..36047
                     /locus_tag="U2K71_000939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7315044.1"
                     /translation="MIKLLVVEDDKVLSNNIKEILAAIGEITQVYDGSEGLFELESGV
                     YDLAILDLMMPEMNGYEVLAKIRKEKIQTPVLILTAKDSLEDKIEGFERGADDYLTKP
                     FYREELIMRVKALLKRSLGLFDENVLKYKDISCDLKSNEVLYKEQVLPIQGKEFELLV
                     YFLQNKGIILTKEQIFDRIWGFDSDTTITVIEVYMSNLRKHLRSTGLDKEIKTLRNVG
                     YILQRV"
     gene            36052..37392
                     /locus_tag="U2K71_000940"
     CDS             36052..37392
                     /locus_tag="U2K71_000940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM7315045.1"
                     /translation="MNGDITRKQRSRFFATNVAAFAFIFFLLGFITLQILNQSAYRET
                     DLSLEKINFDSRTVQLEIQRYQQGDPFLNPTQTEQNRTNMEQPLMEPSNDRFNTQVIL
                     WSKNGEILNKAAIGGRFNQIKELKMDTKNLNTIQNIELNEDSGSSALSFRSITKKAET
                     NQDGVAYIQILENTNQIKNSLATFRTIMILCMIVFWILSIGISYYLSSLSMRPIILSW
                     RRQKEFVENASHELRTPLTIIQNSLEHLFTKPNHTIIEESESIAQALSETRRLTGLTS
                     DLLTIARNDSDQQLLSKQMINTQEYIEQLVKPFQEMAIIDGKEFILHNDATLEVPIDE
                     KKIHQVLVILLDNALKYTNPEDKIILDSSVNHKEWLIHVKNTGPSISEEDQARIFDRF
                     YREDQSRAKETGGYGLGLAIAKQVIEQHHGQISVSNYLPQGVDFKVQLPLKPKK"
     gene            complement(37541..38527)
                     /locus_tag="U2K71_000941"
     CDS             complement(37541..38527)
                     /locus_tag="U2K71_000941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2382 domain-containing protein"
                     /protein_id="HEM7315046.1"
                     /translation="MEEKNLTVIGSYSTREEALSVIERLRNEGYERDDIVIYTTDEAA
                     SRLGFDGLSGIDVETDENRMDGEEDRSLWEKIKDTFSFDTYDSETATPENDPLYQYRG
                     DISDGKFVITVKGYRQPETTEDTMDTQDTVSPASTMGTGLETGTGTDPMDVGGTTGFQ
                     NTTDPLNMTPGTDPMDVPGETDTMGTSKTEDIDRKPNLDDDTIQLKEEKLDVNTHDVT
                     TGEVDIHKHVVNDTETVEVPVKREEIVIERKPVTDQSSQGTDEKLEDDTITIPIKEEQ
                     VDVSKHTVIREEVGIHKEEHEDVEKVTEDVSREELDIDTSGDVHIEDRNKKS"
     gene            39251..40282
                     /locus_tag="U2K71_000942"
     CDS             39251..40282
                     /locus_tag="U2K71_000942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM7315047.1"
                     /translation="MDSKTRKQLQEKLKQRTHLLPSVFQQFLEENRSSLSLGSRYEYA
                     KDFHLFTDYLRKVFSEEVDDQLIIHLEKQDYQNFLAKIYRHTRSFQTTANQETEQLFE
                     NSYYGISRKQYSLNHFLRFLYQKGLIEEEISLLGTSLPETTKAAPRYIDAELFKDFEH
                     LFIPIEGFQTSREASFEEKNRPRNLAITAILLYCGLKIHEVVELKRKDVSLTKQILNL
                     NRKENRLNKVPIPKEAMPFLENYFKLTAQNTDENSFVFRSSHDQQISPRTIRQILSKI
                     TVYKNVSPELCRKTYRYYAELYLDDADAVNYLCGNRYLSSHSFQDIWEKMVDFSYHEL
                     AAFVRVSSI"
     gene            40391..41923
                     /locus_tag="U2K71_000943"
     CDS             40391..41923
                     /locus_tag="U2K71_000943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HEM7315048.1"
                     /translation="MMETITYSPLNLFTNFQEAAANFSETPIIFDEPLSAFPELGLET
                     TYKKCCESVLKRAYQLAHLGVKSGDKVIIYKSSAFDTYLLAVSVSYLGAVPVMTSYHL
                     PTTTMEVFIDRLEDPFILFDDETKKRVREISNGTKSKQIPILYLMEQPALSVSQSFLD
                     KNEISYMTHTSGTTGIPKLICHSAHSMGWRTKWQKTVFTKISEKKLIAFHISPVHSRF
                     NIGISSLMSMGFPMMPLANAQSSKVVHMLEAHRPIALETHPNNFVQWRFTAKEHPEAF
                     SGIRYYHSTFDAINNQTMKTFLRTSMAQDAVFLQVYGQSECGPMILKAHTLESLKTSD
                     ARDMGVGLEDMTSARITDSVGNVLPENTDGHIQLLSKGRALTYYKEDARFQENVYGDW
                     WDSGDYGFMDKHGHLFLKDRQVDLIETINSTLAIEDFLLDSLDFLAEVVIVRDKNHSP
                     QPIIALAPDKEMDWNRWWEQVHELPRLNVPIIRDFDAIPRTATMKVQRLQIEKELKSQ
                     KS"
     gene            42062..42874
                     /locus_tag="U2K71_000944"
     CDS             42062..42874
                     /locus_tag="U2K71_000944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylpyrimidine/phosphomethylpyrimidine
                     kinase"
                     /protein_id="HEM7315049.1"
                     /translation="MKRILTIGGSDPFAGGGIQTDLKTFENFGLFGLSALTSIGALDI
                     NERFMLESISLELLKRQLVSIDKMTSLDGIKIGLLNSSEAIMVVRDFIKTKQNIPIIL
                     DPVLAFKETEAVINQLYISQLIEELFPLVTMITPNLAEAALLSGQALPSSLTEMIHLA
                     KELTASGTKNIVIKGGTRMAGDEAVDLLYSIDHYEVFSHKKIDTTTVNGAGCAFSSAI
                     TANLVLGADFTKAVAESKKFVAHCISNGVLMNDHTGSVWSGGIQKGGMRNEN"
     gene            42864..43349
                     /locus_tag="U2K71_000945"
     CDS             42864..43349
                     /locus_tag="U2K71_000945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM7315050.1"
                     /translation="MKTKQLTIYAMLIALTVALSLTILIPVPATNGFVTLCEAGIYTA
                     AFLYGPTGGLVVGASSGLLIDLISGYPQWAVFSLVIHGLQGYVAGSFSKSSTRMWGLG
                     LLLGSLVMIIGYGIAGWILYDWPAGVASIPGNIFQNLIGMGLAFPITASLQRIQARKQ
                     Y"
     gene            43372..43917
                     /locus_tag="U2K71_000946"
     CDS             43372..43917
                     /locus_tag="U2K71_000946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01440 family protein"
                     /protein_id="HEM7315051.1"
                     /translation="MKLSEKELKDQLTEIVNDILAEAHLKKGDLFVLGCSTSEVVGGH
                     IGKNSSAEVGQWIIRTLKELLDPKEIALAVQGCEHLNRALVVERTVAEAKNFEIVSVV
                     PALHAGGACSVAAFDQFNDPVEVEHVVGQAGIDIGDTSIGMHIKHVQVPVRPRLKTLG
                     QAHVTALRSRPKYIGGPRANY"
     gene            complement(43990..45633)
                     /locus_tag="U2K71_000947"
     CDS             complement(43990..45633)
                     /locus_tag="U2K71_000947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEM7315052.1"
                     /translation="MIQEQKWWKEAVGYQIYPASFKDSNGDGQGDINGIREKLNYLKD
                     LGIGFIWINPIYQSPFVDNGYDVSDYQDIEERFGTMEDFDLLLKEAHELGIKVIMDLV
                     INHSSDQHQWFEESKKSKESPYRDYYIWVDGVDGKEPNNWTSIFGGSAWEYSHETGQY
                     YLHVFAKEQPDLNWESEKLKEELFNMIRWWLDKGIDGFRLDAISHVKKDEYSVKATEN
                     PFSPFQNVSGIEEHLTDLKHVFEEYDIMTVGEASGVTAEEGPQWVGKDGYFDMIFEFD
                     HIHIWQQEKEGQLDVLKLKHALSAWQTSLDGIGWNALYMENHDVPRAVSVFGDTRPDF
                     WAMSAKAIAMMYFFLQGTPFIYQGQEIGMTNMPFESIDQVDAVDSKRLYKRLLAEGKT
                     REEALDIIRETTRDNSRTPMQWTSEQYAGFSTHEPWLITNPNTKTINVEQQEYEPESV
                     LQFYKNMIRIRQTNKGLIYGSYKEYLHEHPQLYVYERYLEDEQYLIMVNLTESLADYE
                     LPKEADQSWTLLLSNSSSGEFEAKGILAPYEARLYKTNK"
     gene            complement(45762..47990)
                     /locus_tag="U2K71_000948"
     CDS             complement(45762..47990)
                     /locus_tag="U2K71_000948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM7315053.1"
                     /translation="MENFSLTENRTFSRLEKQMIWKKPKSHRTSAEEWRIASEIKGNW
                     QDPEMKISNVLLEGDAGSGKTQLAKALSADLQLPYTKVTCFADMDKSDVFGALLPVVD
                     SNKEQDQELLEAIYQTDTLEAVLTLIQNHYGIDPFSAKEKLAELVQRIEADYPDAIHY
                     SYYPSEIVRAIEKGYLLEIQEPTVIRDASVLVALNSALEPNGMLNLPTGIIRRHPDCV
                     IVITTNRNYQGNRPLNESLRDRMQHAEKMDLPALEVMAERAMAKTMIQKQELLLKMAE
                     IIRLLDETAKANAIKGVAGMRSYFYWANTYKQGQDLLQSIYPKVLYKLSTDSDELAIL
                     EQALSDSGLLLELQDILRKEKWGHPQSEHTKERTISAEEANERNITENAEEILRLDEK
                     KSLSAEEPNEGEEKQEETIEKNEIPPQMQKERSSDIEGKNQKEQQTGDDSQEMSASDM
                     EAHDKEIKKQLNKEARQIMKETIHEKEGLIVHRPAFQGKTPEARELHMQVMPIVESLS
                     RQILELLDNEQTETYQKGKYEGQRFNASKVAYGDLRNFDKKNPPHEQPSLAVAVRIDE
                     SGSMIRDDRIEAAKKAAIAIAEFSKKVDIPLLIYGDTADRSSREKTSLFSYKEFEDGF
                     QWLNEKLVTMKPRQNNRDGAALRLAAEKLNRQSATTKLLLNISDGQPKALPDYTGKKA
                     KEDIQSVLKEYERQGILFVSAAIGQDKEEIKSIYGESRFVDITDLNEFPKQMIQLIAR
                     YL"
     gene            complement(48023..48502)
                     /locus_tag="U2K71_000949"
     CDS             complement(48023..48502)
                     /locus_tag="U2K71_000949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315054.1"
                     /translation="MEIPTNLKHKPVIVVEDYDKVDGRNALNMDAKGLSLGLAQWNDR
                     GKVDISGKVWRHTGEKWSRQSEELPITRILDLAILAAQGSLYFQDAYRYEKFYDPENP
                     VVDIIGLQGNRMTVEVDTKNPKIDEDIILYYDTLQKDGELIGERFRILKRLLDELGY"
BASE COUNT        14834 a         8981 c         9174 g        15767 t
ORIGIN      
        1 aggagaaaaa gtagaaaaga aggtgtttgt tatttgggaa gtagttaaat cgagagtaca
       61 agaaatgaat gaacatttct cagtgtcatt cgcagaaaat ccaagtaagt attttcgacg
      121 tcttgttttt ctgctcatct tatcattgct gttaacagtt gcaatgtttt tattgccgga
      181 aaaaatccga gaagagcaag taaatagtct cgtttattcg tccataccaa ccagcaaatt
      241 gaaacctttt gattacagca aagctgatca gaaaatcaaa caaagccggg cgatatcggt
      301 gatgttcagc aaaccgaacg ggaaaaccta tcaggacatg cttgatgttt ttaatgatcc
      361 tgagcttatg gaagaattga atcgaccgat cttttattat ccgatcgttt atgatgtaca
      421 tgaactggaa caaaaatata acatacgaac ggatgaagtg acatttattt tctttgatgg
      481 aggaaaagaa gcaaatcgga taactgcggg taagggagga ataactgatt ttgataagga
      541 gttgatccct gaactgaatc gacttccgtt agccaatatc aaacaattag aagaagaatt
      601 ggatagaacg ggagtaactt cccaaaattc cgagattgct aattgagaca ttggcgcgat
      661 gaaacggtgg gtctatatag cgctattttt aattatcaga acaagtgatg tttgatcttc
      721 aaaataacaa ataaaaaatt aaaaaatata gttgacgaaa ttcaaaaatt cgattagact
      781 taaatcaatt cttaaggaaa gggtgacctg taatgaactt atcaaaacgt atttcagcag
      841 aaaacaaaaa aaattggcgc agctggcgta gataagttgt attaatggtg agactttccc
      901 atagatacaa ctttttagag tgcatttcta aagcttgtat ctatgctaga catgaacatt
      961 gactgccgca tagattagaa tctatgcggt tttttgtgta aagacaatga ccgctttggc
     1021 cattgtcttt ttttgtagac tggatacttg cctgattata ataaataaaa agattgactt
     1081 tgcaggagga aaaaaatgaa aaacaaacga ttaataacag ttgtagtaat tattttagtt
     1141 tatttagcag gtgcatttat ttatggaaaa gcctcaggcg aaaaagaaag tacttcgacg
     1201 aaccagacaa aaaaaacaac agttgttggg gtattgcaat acgtgagcca tccggcatta
     1261 gatgagattt atcgaggaat caaagatggt ctgaaagaag aaggattaaa agaaggagaa
     1321 aatcttgaga tcaaatttca aaatggacaa gccgatcaga gcaagctagc cacaatgagt
     1381 caacagctag tacaatctga tccagatgcc ttgattggaa tcgcaacacc tgccgctcaa
     1441 tcattagcaa acacgacttc atcgatccca gttgtgttag gtgctgtaac ggatcctgtg
     1501 ggggctggat tagtcgcttc tttagagaaa cctggtggta atgtaacagg tgtttctgat
     1561 cagccgcctg ttacttcaca aatcaagctt gcttctgatt tgttgccaaa tgcaaaaaaa
     1621 gtcggaatct tgtatgcttc ttcagaggat aattcaaaat accaagtagc acaagcagaa
     1681 gaagctgcaa aaaaagaagg gttagaatca gtaaaatatg cggtgccttc cactaatgag
     1741 attgcacaaa cggttcaagt gatgagccga caagtcgatt ttatctatgt tccattagat
     1801 aataccattg ccaatgcgat gcaaacagtg gtaaaagaag caaacaaagc aaatatcccc
     1861 gtcattccat ctgtggatac aatggtggaa caaggtggac tagcaacaat cgggatcaat
     1921 caatatcaat tagggcttca aagcggtaag atggcggcag aacttgcgag tggaaaagaa
     1981 aaaccggaaa cgacgcctgt ttatacattt gatcaagggg atacagtgat caatcaatct
     2041 caagctgatc gcttaggaat cattgttccc caatcgatga aggaaaaagc caaaatcatt
     2101 acagatgact cgcagcaaga aacatctaag gaggatgaca aatgatcgta tcagctattt
     2161 cacaaggcat gctttgggca atattaggat tagggatttt catgacttat cggatcttag
     2221 actttcctga tatgacgaca gaagggtcct tcccattagg tgctgctgtc tgtgtgacag
     2281 ctattactca tggggtagcg ccgattgtag ctacattatt aggcgtaggt gctggcatga
     2341 tagcaggact cgttacgggg ttactttata caaaaggaaa gataccggtt attttagcag
     2401 ggattttagt gatgtcagga ttgaactcag ttattttgtt tgtgatgcga acaccaaatc
     2461 tttctttatt gaatcatccg attctacaag acagcttcca tcgctttggg ttacctgatt
     2521 actttgacac agtccttctt ggcttcatct ctttgggcat cttgattgct gtactgctat
     2581 tcttcttcaa tacagatttg ggtcaaggct atattgcgac aggagacaac gaaacgatgg
     2641 ctcggtcgtt agggataaaa accgatcgta tgaaaatttt aggtttaact ttgtcgaacg
     2701 gtgtcattgc attatccggt gcgctgattg cccagaacga cggatatgca gatgtaaata
     2761 aagggattgg cgtaatcgtc atcggtcttg cttccatcat catcggtgaa gtgatcttcc
     2821 aagaattgac gctagcagaa agattgattg ctattgttgt cggcagtatc atttatcagc
     2881 tattgatttt agtagtaatc aaactagggt ttgacacgac ttatctgaag ttgttctccg
     2941 ctatcatctt agctatttgt ctgatgatcc cgcaattgaa gaaagcactg aacttaaaaa
     3001 cgggttttga gaaggaggca tagtaatgac tgatatattg acaatcaaaa atggcagaaa
     3061 agtcgtaaac aatggaatga atgaagagaa agtactattg gatgaaatta accttacaat
     3121 cagagaaggg gacttcatca ctgtccttgg cgggaatggt gctggaaaaa gcacactgtt
     3181 caatacgatc gctggtacgc ttcaactcag tcaaggatcg atctctttta aagatcagct
     3241 gatcacaaaa gattcagaag aaaaaagagc acgattcctt tctcgagttt tccaagaccc
     3301 caagatggga acggctcctc ggatgacagt agcagaaaat ttgctacttg cacaaaaaag
     3361 aggtcaaaaa cggaaattcc gtttacgtaa cttgaaaaat caaaaagaag attttttcag
     3421 actatgtcaa gaagtcggta atggcctaga gcaacacttg gatactccag cgggtgaatt
     3481 atctggtggt caacgccaag cattaagctt gctgatggcg acgatcacag ctcctgagtt
     3541 actattatta gatgaacata ctgcagcttt agatcctaaa acagctaaag cattaatgcg
     3601 attaacaaat cagcggatca atgaacaaaa attgacttgt ctcatgatca cgcatcgaat
     3661 ggaagacgcc ttgaaatacg gaaatcgtct gatcgtactc caaaaaggga aaatcgttca
     3721 agatatttct gaaaaagaaa agcaacattt gacaatgggc gaccttttgc agttctttga
     3781 agaagtagtg gattgatttc gacagaaaaa ttcctttcag tagaaggact tgaaaactca
     3841 ttgacatctt cctttgattc atcctatact taaaacaata aacaattaaa catatttggg
     3901 tgttttgtag gatcacgtag tcaatgattt taacaattaa atagaatatt ctttgctctg
     3961 acaagtaaac gatgagaaaa gaatttgcta ctttgccacc tagacgtttc gtactttttc
     4021 gtatcaaacg ctaagtggct ttttttattg aagtaagaac aaaggaggaa taagatgatt
     4081 tctggacaaa ccaaattagc tgggctcttt gctgatccgg cttctcacag tttatcccca
     4141 ttgatgcaca atactgcttt tgaagcaaac aacatcgatg cggtgtattt agctttccaa
     4201 gtaaatccaa tgaatttgaa gcaagcagtc gaaagtattc gaacttttga tatgttaggt
     4261 gtaaatcttt cgatgccgaa taaaacagcg gttattcctt atctggatga ggtaagccag
     4321 gaagcgcaat tgatcggagc agtgaatacg atcgtgcacg aaaagggtcg tttgattggc
     4381 tataataccg atggtgcggg cttcatgcaa tccgtgcaag aagctgggat ttcgatcaaa
     4441 aagaaaaaaa tgactctact gggagctgga ggtgcagcaa aggcagtcac tgttcaagct
     4501 gcgttagacg gcgctgagga aatcgtagtt tataaacgaa acaaccaaac attcgctcaa
     4561 gtaaaagaaa cattcgaaca actcagctat cgaacttctt gtaagataaa agttcgtgac
     4621 tttgccgata cgcatcaatt aagaaaagac ttgaaagaaa gctgtcttct agtcaatgca
     4681 acagatatcg ggatgggaaa gaaaatcaat cagtcaccgc tcttagatat aagcatgatg
     4741 catgagaatc tagcggtttt tgatttgatt tatgcaccta gagaaacacg tttattaaaa
     4801 gaagcaaaaa aagtaggagc aaaaggatac aatggacttg gcatgttgat ctaccaagga
     4861 gcaatcgcgt ttgaattatg gacaaaggaa aagatgccga tccatgtgat tgaaaagctg
     4921 tttgattaaa aaaggataat agataaagat cttttattct cgcataaacg gtctcaagaa
     4981 aagtaatcct ttatgtatcg aagcatttat ttcgttattt tttattaaat tatcaaaaaa
     5041 tgataggctt ttgtcgattg gttatcgtat cctttattta tcagtaagaa acgaggagga
     5101 accacaatga gtcggatgga tcgctataag gacatccata agaaggcgaa acctctgaag
     5161 cgggatacgg ggagtatttt tgctagaaga gaacgaaaac gaacgcgtat ggaaaatgag
     5221 gaagatgcta cccgtattta tgaaagagag cagcattcat ttgaacattt gaaccaatac
     5281 gagcaacaga tatacagaga tgcaccaaga gcagaagaac aacaaaaaaa taaaaaattt
     5341 ttcaatcggc aaaagaagaa caaaccgtta aaacctcgaa gaaagcgatc gtggaaaaaa
     5401 atcattctat cgattttgct tgttttgatc ctttattcag gtatctcatt ttttattggt
     5461 aaatatgtag ctgaacatga cacaagctta gaggcagcga cttcagaaac ctttcatggg
     5521 caaaaagcga cgaatggtgc gcataatatt ttgatccttg gaagtgatac acgcggagaa
     5581 gatgccgggc gagccgatac gatcatggtc ttacagttgg atggtccttc ccataagccg
     5641 aaactgattt cttttatgcg tgacagcttc gttacgatcc caggggtagg acaaaataag
     5701 atcaattcag cttatgcata cggaggagcc gatctagtgc gtcaaacttt agtagaaaat
     5761 tttggaatcg attgtcaata ctatgcgaaa gtcgatttca aatcgtttga aaaagtgatc
     5821 gatgccttat tcatgaacgg agtgaagatc gatgcagaaa aagatctgaa tctggatggt
     5881 gtagatatta aaaaaggggt ccagaagatg gatggacatg ttcttttgca gtacgcacgt
     5941 ttccgaatgg atgaacaagg agactttggc cgtgtcagac gccaacagca agtaatgaac
     6001 gctattttca gccaattgaa gaatccattg aatctgatcc gtgcacctta tgcagctggt
     6061 aaagctttag gatatacatc cacagatgtc actgcttggt tcttgacgaa aaacctcttt
     6121 tcgattgcac gaggagctgg tggcattgat cgaataagtg tgccagtaga cggttcgtgg
     6181 aattatgggc aaagtgatta tgctggcagt attcttgaaa ttgataatca aatgaatcaa
     6241 tctgctatta cagatttttt gaataaatga tcataaaaaa gatcaagaag aagctgcgca
     6301 aggcttttgt catgaatcaa tcaaacgatt cactcgtaaa aaacgctttc ttatggtaga
     6361 tttaaccaag agaggagctg tttacattgg atatctatct gaacttcaaa aatgagtcgc
     6421 gtgaagccat cgctttttat gaaaaagttt ttcagactga agcatcaggt atcaccacat
     6481 acggtgaaat gcctggaata gaacaaatga acttgccaga agaagcaaaa aatctaatca
     6541 cgaatgcacg gctgaacatt tatgatacat tagttatgtt tgcagatacg ccagaaggaa
     6601 ttggtcctga attagttgtg ggaaataatg tttctttagt catccagaca agaaatgaag
     6661 aagaaacgaa gcgcctattt gaaagcttag ctgagggtgg taagattgag atgccactgg
     6721 atgctgttcc gtgggctcag ttgtatggga tggtaactga tcgatttggc atcaactggc
     6781 agattaacca agagtgacag ctaaagatga atattttctg gaaacatcaa gcagcaaacg
     6841 gatcatcgtt tgtttgcttg atgtttttgt gtctatttcg actatacggt atcttttttt
     6901 tattgccaga tgcgaaaata tgttccataa tagagaagtc cgcaaaaaat taagtaacgg
     6961 aggtcagaag atgaagacgg tgatactagc tgaaaaacct tcacaagcaa aagcctatgc
     7021 cgaaaccttc caacagcaaa agcggcatga agggtattat gaaatcagcg atccattgtt
     7081 tgctggtgaa gtaaccatca cttatggttt tggacatttg gtagatatgg ttgctccagg
     7141 agcttatgaa gagcgttggg caaaatggtc tttagaaaat ttgcctattt tcccagaaac
     7201 cttccgttac gaagttccaa tggacaaacg tgcacaattt tcgatcgtaa aacgagaatt
     7261 gcagtcagcc gataccatca ttattgcaac agatggtgat cgagaaggtg aagccatcgc
     7321 gtggtcgatt attcgacaag caaacgcatt ttctaaggaa aagaactatt tacgtttatg
     7381 gatcaattct cttgaaaaag aagcaatcta tgaaggtttt aagaatctcc aacctggaaa
     7441 gaactatttt ccaaaatata aagaagcgca agcaaggcag aacgctgatt ggctgattgg
     7501 aatgaatgga agtccattat atagcttgct acttcaacga aaagggattg atggcagttt
     7561 ttctcttggt cgtgtacaaa caccgacact ctatatgatt tatcagctgc aagaagaaat
     7621 caaacacttc aaaaaagaaa cgtactttga aggagaaggt ttaattacga caaacaaagg
     7681 gcagttttct ggaaaaatcg taccaaaaaa agcatttaaa acccaagaag agttaacgaa
     7741 ggaaatagaa aaacttggag cgcatttagg taagcaaaac ggacggattc ttgaagttac
     7801 gaaaaaggaa aagaggttgc atagtccacg tttattttcg ctatctagtt tgcagacaaa
     7861 gatgaatcag ctaatgaaag caagtgccaa agctacactt gatgctgctc aaggattgta
     7921 cgaaagcaag tttctaagct atccaagaac ggattccttt tacattacag aaaatgagca
     7981 tcagtatctg gttaaacatc tgcaaaatta taaaaaattt ttaggaatag aagaagtaca
     8041 gacaactgag atcaaagcaa agaaacgata tgtcgatgcg aaaaaagttc aagaacatca
     8101 tgcaattatt ccgacaaaga cgattccgac tcctgcacgc tttgcgaagc tttccaaatt
     8161 acagcaagca atctatttgc aagttttgcg cactactgtg gctatgtttg ctgcagatta
     8221 tatctacgaa gagacgaccg taatgacggg cgtacagcaa ttaaagctaa aaagcatcgg
     8281 aaagatccca ttacagcaag gttggcagga aatcttacgc atcaagtcaa aaaagaaaga
     8341 agctcagact cttcctccta tcgtagaaaa tgaagaagca gaagtagaac tgaaaagtat
     8401 ggagaaagaa acacaacctc caaaacccta taccgaagga acgttgatca cagcaatgaa
     8461 aactgcggga aaaacgctgg ataatgatga agcgcaagaa attctcaaag aagtagaagg
     8521 aatagggaca gaagctacac gagccaatat cattgaaaat cttaagcaga agcattacat
     8581 cgaagtcaat aaaaacgaaa taactgttac gccaaaagga atcactcttt gtaaggcagt
     8641 agccaatgaa ccattactga cgagcgcaga aatgactgcg cgctgggaag ggtacttgag
     8701 aaaaattggt aaacaagagg ggacaccatc tgttttttta gagaatatca aaaaattcat
     8761 cctacattta ttggcagaag ttcctaatca aatcgacaag gtagactttt cagatgagat
     8821 tagcgggaca cgtcagataa aagcgatgga gaagaaaaac gatcaattag gaagatgtcc
     8881 aaaatgtaaa aagggaatag tcatgcttta tcctaaaatc gctacatgct tgaatccgga
     8941 atgcgacttc aaattgtggc caacagtagc taaaaagaag ctgactaaaa cgaatctcag
     9001 agatttgctt tcaaaaggaa agacaagtaa agttgtcaaa ggttttactg gtaaaaaagg
     9061 gaaattcgat gcagttttag tattaaaaga agatatgaca atcggttttt cttttccatg
     9121 gatcgaaaac agtggaacaa aagcagttga tggaaacaaa gaaacatcaa gaaaaaataa
     9181 tgaaaacgaa gtagagtaaa tgaaaacaat gaaatagcgg aatatttatt ccgaaaagtg
     9241 agaatataaa taccaataaa actaaaaaat atcagtgaat caagcaaaaa cgttggggta
     9301 ggctgatttt atatagatag caaggagttt ttttatttga aaaaagtgca ttcattttta
     9361 aattatctta tgggagcaat cacgattgtg gctgtgtacc aagcatttct aaagtatcct
     9421 gattacggga cgaagttgtt gtcattgacg cacaatcaaa cagcatattc accaacgttg
     9481 attttatcga ctttgactgc aggagctaaa agcgctactt atttttttca taaaacgaca
     9541 agcatgctaa agcgaaagaa acagcgaaaa caattgttgg tggtggtagc ggctatgatc
     9601 attatttatt ttctaccagt tattacacaa gtatttcatc aatattttgg ttaagaatac
     9661 gagagtttgt gacaaaagtc atgtcacaaa cttttttctt tgtcattttt tcgtaagaaa
     9721 aagcaaaata aaagtatata cattgattaa aagtatatac ttggatataa tgtacttact
     9781 atagaatgag aatggtatag agggtggcga aatatgaaaa aaatgaaaaa gtataaaatt
     9841 agaaaatctg gcaatagcga tgtaacaact atcccaccag aagtaaaaga attcatgggt
     9901 gtgcagacag gtgacgcaat cagttatgtt tttcaatctg atggttctgt acgaatgatc
     9961 aaagcacaag aagaacctga tattgattca ttagttgatt cgatcatgaa tcaatatgaa
    10021 gacgcgttaa aagatttggt tgatttatga ggtatctttc gactaaagaa attatcaaaa
    10081 tcaacgcgaa agttatttta cgtcactcgc caggcgaaat gatcggtgta aaagatgcaa
    10141 atgcattaga tatggccgtc aaacagcctt cacaagcagt attcaatcaa gaactctatc
    10201 ctgaaatcta tgaaaaagcg gcgattttag caattaattt agctaaaaaa catccttttc
    10261 ataatggcaa taaacgaact gctcttgttg ccatgttgtt gtttcttcaa ttgaataatt
    10321 gtccagttgc tttttctcgt caagaagctg ttgattttat cataatgatt accactagtt
    10381 cattggattt cgattcacta aagtccaaga taaccagtta tttacgacaa accgcaataa
    10441 aatgaacgaa ttaaaaactt tatttctcaa ttagatcagc atgtcattaa ggaatatgct
    10501 gattttttta tgtttaatca tacaaaatag ttttttcata aaaatatgga aaatatggat
    10561 taattttgaa ataattgatt taaaaggaat acaaaagagt gaatagtagg aaatagtggt
    10621 ttagaactag tgggctgatt ttttatatat tttagtttac ataatatata ttatacaaag
    10681 tagatttaaa aaatgataaa cctctctcga acggtttttt atcaaaaatc gtcatttttc
    10741 catcaaaatc tgcttatgca aaattataaa aaaattttgc agtttttctt actttttttg
    10801 tcgtttaatt gaatgataaa aaccagtttc tttattcagt taatcaaata tgaacgaatt
    10861 agatatgaat cattgtattt gccgttgcgg aaggattttt taattaacca cagataaaca
    10921 ttgataacga ttcgcttatt gtaatcaaca gattaaattt catatgttca tatgtacgga
    10981 tacattttcg gaccgattaa ttttaaccat tctatttatt tgcttaccta ttagatatca
    11041 ataaaaaact gcttaacagc tatttcagct gttaagcagt tttaggattt ttagttcata
    11101 ttcattgttt cttataatac agtgttcaat acttctctta aagcacttgt tgatttgctg
    11161 aacattgctt tttcttcttc gttgattgat aattcaacaa cttcacgaac accattttcg
    11221 tcaacgattg atggcacacc tgtaaagata tctttttcgt cgtattcacc agttaaataa
    11281 gcagatacag gcaatacagc ttgttcgtta ttcaagatag cttttacgat acgtgcagta
    11341 ctcataccaa taccatagta agttgctttt ttacgatcaa tgatctcata agcagcatta
    11401 cggactttat cagcaatcac atctaattca tcttgggcaa tacgatggtc tttttctaca
    11461 atttcaaaca ctggtttacc accgacagtt gtatgtgacc atgcagcaac ttctgaatca
    11521 ccatgttcac ctaagatata gccgtggacg ctgcgtgggt caacttttaa tttcaatgcg
    11581 atttctttac ggaaacgagt tgtatccaat gttgttccag taccgataac acgtgatgtt
    11641 ggcagaccag attcgttcca agcgatatat gtcaaaatat ctactgggtt tgaagcaaca
    11701 acgataatac cgtcgaatcc tgatcccatg atttctttta cgatttggcg catgattgac
    11761 gcatttgttt taactaaatc taaacgagat tgaccaggtt tttggttgat accagctgtg
    11821 ataacaacga tatcagcatc tttacattct tcatagccac ctgaccatac agccacattt
    11881 tcatcgcccc aagccatacc atctaaaagg tccaatgctt ccccttctgc tttttcttga
    11941 ttcacatcga tcaatactaa ttcattagag attccttggt tgatcattgc ataagcgata
    12001 cttgttccaa caaaacctgt accgacgatt actactttac gacttgtttt tttcatgaat
    12061 aaacagctcc ttttcataac tatttgttac tttcatgaaa agtatagtct tgtttattca
    12121 ttttatcaac atgttaatta taatagttgt tattataatt tttgatggaa acgaattcct
    12181 gaatacgcat aacagataaa tgacaaatgt ttacacctat atcccctgat aatacaacca
    12241 aaagtttaga actgaaaata aatccaaaaa aattcgctgc aacgatataa taagacagca
    12301 atcgcaccta aaacaatttt ccttcatatg ctttagtgaa agattttctt cttaatgttt
    12361 atttctccat tcattgggag tacatgttga atacttcttg aataatttga ataaagaagt
    12421 cgtttgttta tatccgatac gattagcgat ttcttccaac gaaaaactcg tttcttgaat
    12481 cagttccaac gcaaagtcga attttttctt tagaaccagt tcttgaaagg aagcatcaaa
    12541 atacattttc aatttattac taagatagac cggattgtag ccaaaatgag ctgctgtttc
    12601 ttttaacgta caagaagtaa aatgcttatc gatataatct aatactctaa gcatctcatt
    12661 ttctgcgcat ccatctgctg atgaaaagct ttgttgttct aataaatgag tcaattcgat
    12721 cagtaatagt gatgtataga gatttaacga acggatctta tgactgtatg tatcaaaata
    12781 aatcaacagc atattttcaa tcaaatgcgt caaacgactt gtttttccgc cgcgaaaatg
    12841 aatgaattgt gtatgttccc ctttttcttt tgatgcattt atcaaaaaag aagaaagtag
    12901 attatttgtg cttgccaaat gattcaatat ctctgtagat aaggtttctt cttttaataa
    12961 gatgttgatt aaaatatcct cttgccctgt gtattcgatt ctttgagtag cttctttatc
    13021 aatgatcacc acgtcacttt cttccatctc tatcctttct ccattaataa tttgtaaaca
    13081 tgtaccgcta tacatataat tgatttcaac aaaatcatgt gtatgctcgg gaacttccgc
    13141 atatcgacgg tgtttactga tatgaatatt tccctgttga aatagatccc ctcggtggat
    13201 ttttggcggt gtctgaagat ggtaagctaa gtttaaatca ttgatattca ttccttgttc
    13261 acgctgcaga gtttcgattg attcttcgtt acgcaaaaaa aatgtctaat tcagctagtt
    13321 ccaattgact ccctcctttt tgtaagcggt ttctttcgct tattagttca cgctgtactg
    13381 ctattatatg caaagctatt tttggtttca taaagacttt ctatggttgg gacatgaact
    13441 tcctgtgatt ccgctattga gagaataacc acttcatact atatttttga taagcgctta
    13501 caaaaatgaa aggaacatgc ttatgaataa aaagatgatg aaattttctc tgctgtcagt
    13561 ctctcttatt ttgacttctg ctggtgcagt cacgtctgca attcccagta tgtcagctac
    13621 ttatgccacg cgttctttat ctgctgttga attattaacg actgctccct cttttatggt
    13681 cactattttt gttttgatta gtagttttat tgctaggaaa atcggacaaa aacaaactgt
    13741 gatcatcgga ttagtgattg catcgatttg tggtatcctc cccctcttct cacaaagtta
    13801 cagtattgtc ttagcttcaa gagctggact aggtattggc tttggattga tcaattcttt
    13861 ggcagtcagc atgattgctg ccttttatca cggagatgaa cgagcctcta tgattggttt
    13921 tcaaagtgca tttcaaggat ttggtgcagc tttaatgaca tttgttgcag gacagctagt
    13981 acagttcggt tggcaatata cctttttagt ctacgccatc agcttaccta ttcttgtctt
    14041 gtttgttttg tttgttccta cacctgaaac aactgaactt caatcgcatg gcacagcccc
    14101 aacaagacaa gtaatcaaaa cgaatcctaa aatgattagt tatggtattt ttctcttgct
    14161 ggtcatcgtg gtatataatg ctgtatcgat taaattagcc acagtcctga cgacttattc
    14221 tctaggaaac gaaactaatg cagcgaatat cttaacaatc atgcaattgg caagtatggc
    14281 tgccggagtt tgttttggct ggcttcagac aaaagcacat cgctatatgc ttacactttc
    14341 tttactactg atgggaatcg gctttgtatt gattgccact gtgataaacc tgtatgtgat
    14401 tgggtttggt gcgatcctaa caggaatttc tttttcgtta ttcattcctt acttattcaa
    14461 ccaagtaagt atccaagcgc ccaagtacgc ggagagtttc aatacttcaa tcttattggt
    14521 tggcgctaat ttaggtactt tcattgcacc atatggtttg ctctttctct cttttgcttc
    14581 tcttgggagt aaaacacttg ctgttttcgt caatggcagt atcttgctgt tgattagcgc
    14641 gcttattagc cttctagtag tacacaggac gaatagaaag acagtgataa gagtagatga
    14701 agaaataaaa gaacaattga caacaaaata aatttgatgg aggtcgtgta aaatgacaaa
    14761 tgaaaaacat aaagatagat ggctttggta tcctggagac tttgagatcc gccatggact
    14821 gctacaaaat ttccaacggg aagaacgagg ttttgactgg ccagcgtact ggtacatgga
    14881 tgattgccat cggaacgtca agtttaagag atattacttt cttgatcaac cttccatgtt
    14941 taaagtgacg atccaaggag ttgggtatgt agaaattaat ggacaaaaac atccatgtgg
    15001 aaaatggctg acttgtccag ctggtaaagc aaaaattcgt atctttgtag gacatacatc
    15061 agggctacca gcgatgttta tcgagggaga cgaagtaaaa tcagatattg gctggacagc
    15121 aagtaatttt attgaagaat atccagctgg ctggagtccg ctttatgtcg atatcgcaaa
    15181 agatccaaat caaatctatt atcaaaaaga atgcgtccat ccagtcactg aaacggagat
    15241 tggcggagga atcttattcg attttgggcg agcagtgaat ggctgtgtag aactaatatc
    15301 tctggatgac catatcgaat ctatcactct atgttatgga gagtccgatg tagaagctat
    15361 ggatatcgat tattgctact ataagcaaat aggaatgaca agaaaaatga aagctcgtaa
    15421 acgcgctttt cgctatttgt ttataccaga ggtttctact ggaatggttc aacttcaagt
    15481 ttttcatgaa tttttatctc tttcgaaaca agctgctttt tcaagcgatt cttctttact
    15541 gaatcagatc tggtcagtat cagaagaagc cttcaagtta tgtagtgatt tgttttttat
    15601 cgacggtatc aaacgagatc gctggatttg gtctggagat gcgtatcaat cattcatgat
    15661 caatcaatat cttttttttg acgaggaaat caataaacga acgatattag cattaagagg
    15721 acaaaatgaa atcaagcagc atatgaatac tatcgttgac tattcgatct tgtggttgat
    15781 tggtatcgaa aaccactata tgatgacgaa agactatgaa tttttacgca tcgtctaccc
    15841 taagatgcaa tcattaatga gatacttgat ggagcaaacg aatgaattag ggtttattta
    15901 tgggagagca aaagattgga tatttattga ttggtctgaa atggataaag aaggaacgct
    15961 tgctgcagaa caagtcttat tactgaaagc ctatcattcc atgatcactt gcggagatat
    16021 cttagctgaa acatatggag agaacgaaaa ttatcatact catctctatc aagaaaaata
    16081 tcaactattg aaacaaaacc tatttgacta cttttgggat gaagaacttg gcgcttttat
    16141 cgattgctat gaatctggga gaagaaatgt gacgcgtcat gcgaatattt tcgctgtttt
    16201 attcgatttt gctaacaaag accaacagca atcgatctta aagaatgtct tattgaatga
    16261 cactatcaca cagattacta ctccatattt caaattctat gaacaagatg ctttatgcaa
    16321 actcggacag acgaatatcg tctaccaaac gattcttgat tattggggag ggatgttatc
    16381 tcatgatgcg gtaactttct gggaagaata cgaccctact caaacaggaa atgaaaagta
    16441 cagtatgtat ggagatcctt atggaaaaag cttatgccat gcatggggag caagcccaat
    16501 ctatctttta ggacgctatt ttatcggttt gtatccaaca aagccaggtt atgaagaatt
    16561 tatgatacaa cctaatctag attcatttga aacactgaat tgtacgctac ctatcaaaga
    16621 tggaaagatt tcgcttgaaa aaacaaaaga taagatcgtg attacttctg atagaacagg
    16681 cggaacagtg aaaatgaatg gtaaaagtta tcctcttcta gcaaaacagc cacttgtctt
    16741 aacaagctat tcaaaaataa atgcataaaa caaaaaactg cttgcgacat atcagaagac
    16801 tttaaatctt cttctgatat tgtccaagca atttttattt tgaagtttgc ttaattctgt
    16861 tctttagaaa caatgtttgt tctttttccg gcaaaaagaa agctagataa tacgacaagt
    16921 aaaaagaaag cgatgattcc aactgtcaag gctatttgac cgtattctcc tagtttccca
    16981 agtacaagtc ctggtactag ataatcagta tcggcaaatg tagaagaagc gattttaaga
    17041 tctcctaata ctggaaggat aaataatggc agccaactaa tcaataagct attgaaagca
    17101 gaacctaata ccgcccctct tctaccgcca gtcgagttgc cgaagacacc agaagcagct
    17161 ccgcagaaga aatgtcctac taccccagga atgataactg tagtttgcaa gcctatcata
    17221 gcaaacatgg atagtgttcc tacaaagaag ctaacaaaaa agccaattaa tacagcatta
    17281 ggtgcatatg gaaaaacgat aggaacatct aatgctggtt tagaatcggg tacgagtttt
    17341 ttagcaatcc cttggaatgc tggaacgatt tctgccaaca ccattcgaac cccttgaagg
    17401 acgataacaa acccagctgt gaatgttcca gcttggatca gtgcaaagat tacagcgttg
    17461 tctccattgc ttagttctct ttcaacaaat ccaggaccag ctaacaaagc aagtgcgaca
    17521 tatacgatcg acatcaagag catgatcgat acagtagaat ctcgcaaaaa gctgaaactt
    17581 ttaggaatct ttatgtcctc tgtagaacgg tctttgtttc caaaaaactt tccgatccat
    17641 ccactagaag cataaccgat atttccggta tgccctaacg caacttgatt gtttccagtg
    17701 atgttcttca taaacggttg acaaagagca ggcgtaatag ttaatagtgt tccttcgaag
    17761 accccgccta ataagatcat ccacaaattt gcagtaaacc ccgcgcttac taaaatgact
    17821 gctgttagac aagaaacata aagcattgct tgacctgtaa gaaaaatgaa tttatatctt
    17881 gtgaatctag caagtaaaat attcacgatc atccctacta gcatgatcaa agcagtaggt
    17941 gtaccgtatt gttgcaaagc aatcgccact actgcttcat tgctaggaac tactccttga
    18001 gtatgcagag ctgtttggaa catcgaagca aaaggggtta gtgaattact gattaaacca
    18061 gcaccgccga tcagtactag gaatccgaca aatgttttga tagttccttg gatcacttct
    18121 gaaaatttct tcttctgaag aacgagacca agcagtgcaa tcaaagaaac gaggatggag
    18181 ggctgactga cgattgacac aaacaaattc aacaatttat ccattgatag ttcctccctt
    18241 gaatcatttt tattcttttg acaagatctt cgttactttt tcttttactt cttcgctgtc
    18301 aatgatcgat tgcaaaggta cgagcttttc atcaggtata gaagttagcg tatctttcaa
    18361 agaatgctca atgaagaaat aatcagccat atcgctagat acacttccaa catccgtatg
    18421 acttacttca aactcgtcca catctgctcc aagatctgca agtgcttgtt tgatgttcat
    18481 ctctaccata aaacttgttc ctaatccact ttgacatact gcaaaaattt tcatatcatt
    18541 cgtctccttt tcttatgatc gctaatattt cttcttttgt tgatgcgtgt aataaatctt
    18601 gaagattttg tttattagaa agtagctttg ttaaatttgc caaagcccga agatgtgcct
    18661 cattatcgat agcagctaaa cagatgaaca aactaatggg atgggctgga ttatcaagca
    18721 gatccacagg ttgttgaagt ttcagcaaag acatccctac ttcgtttacg ccatcttctg
    18781 gacgagcatg aggtaatgcg atgtgatcgc caatatggat aaacgcacca tactgctcca
    18841 cgcgctcgat catagctttg atataacttt cttctatttt attttctgat aatagcggct
    18901 gtgcggcaaa tgtgattgct tcttgccagt tttttggttg atccgataat tggatcattt
    18961 cttctgttaa taattcagat agcataggac gttgatcctc cttcttttct aaaagtttgt
    19021 tcattttacg atttaatact tgatagagct tttcttcgtc tacgccttct tttaattcga
    19081 tataaggcag tagcgccgac aacaattctt gggcagaagg cattgaaaat cctggtatca
    19141 gccaatctct ttgtacttga ttgatcagct gatttttttc caagtcagtc atgattggat
    19201 gcagaacata gagagacttt ttcttatcag taagtgtgag aggtacagta gaaaaaataa
    19261 tatcgtaaga agtttcgtca acctcgtgta agcggtcagc tgaattagtc tctgtaaaaa
    19321 agatcatcgg gaataattgc tgaagttcac tttttaagat caatgaagag ctaatgccac
    19381 ttggacagac gatcacggct cttatttttc tatttctttc ttctgcgtcc gcttttctga
    19441 tctctccgcc aaatagtata gtgaaaaaac caatctcttc ttcagggatg ctcttcccta
    19501 ttcgtctttc taacggaagt aatgcttttc ttgtcatttc gtagatggaa gcatattgat
    19561 gcttaatttg atcgatcatc acattaggta aataaaaaga ataggaaata cgaaaatagg
    19621 cagggaccaa gtgataaaag acattcatca ataattcacg aaacgaagca aattctacgg
    19681 ccatcaggaa ttccatgcga tggatgactt ctgaagaaca ttccagcaaa aaatcaagtg
    19741 caggctcctg gacatctcct tcaacgatcg atgctaagca aagagagaaa aatataatct
    19801 ccgaattttg atgttcggaa tagccaaggc cagtcaatac ttgtcgacta atgtcagtga
    19861 ctgcaagaga agccaacagt tcagcatctt ttaccagttc catggatgtt tttccaattc
    19921 gaatggttgt caaactcaaa taataagcta attcatccat tcgatttggt gcgaatcgaa
    19981 gatctgatag tgaaatcgtt ttatctagca aatctctaac tttggcgtaa taatcaatgg
    20041 aaagggttgc ggttaaatac gtgagcccaa atcttccagc aagtgactgc atcaaacgag
    20101 cagtgcgatt ttttgcttct cttcttaata ttgcttctga ccctagcagc ctaaaaccag
    20161 ttttcctagt atacgacagc tgacaatctt ccacttctaa ttcatttctt atctttttga
    20221 tatctgacag gactgtccct ttagaaacct gaagaaaatc ttgaaaatga aaaacagaaa
    20281 cagacgttgc agtcgaataa accatcaaat aaagaagatc tcttcgctct tgttcggtat
    20341 acacagcttc aaatcgagta atatccaaaa atttttctgc ccagtcgatt gctcggaatt
    20401 tctttatacg gatacgggta tcatccacat attcggcaaa aggagttaat tgtttgatcg
    20461 cctcgcttgt atttacttcg atgatctctg ctgctttttc tagactgatt tctcttggtg
    20521 ttttcaggag ataactaatt ttgtagttca cagcgctgtt cacctcctac ttatattcta
    20581 gtatggtagc gcttttaaat cgaggggtgt ttggtacaac ttacagttgg atttttttta
    20641 cgactgtacc gaacgatttc tatcgaatgg atacagaaaa aaagcaggtg accaaatgtc
    20701 tcctgctttt atgaattgat ttcgtccgat caatgatgat catgtttatt ttttgaggtc
    20761 agttcttcaa atttttcttt gacatcttca accgcttttt cgatatcttt ttcaaagtga
    20821 ttgggctcat taggctttgt ttcctcttct acagaatttg aaccagataa agctgtttct
    20881 ttattttctg tttcaccaga gtttgtgcca gctaatgatt cttctttgtc ttctgtttca
    20941 ccagaattag ttcctgctaa agccacttct tcttctgttt taggggccat tgaacctctt
    21001 aatcgtttgt gtttttcacc gtcataattt gtcatgttgg ttcctcctag tagttctata
    21061 ctttttcatc gtacaataag acagtttatg cgtcaaaaaa caaacccgat tcttacaaat
    21121 atgtcatatt tttccttctg attcttgaat gcttttgatt ccttcttcca aaattttcat
    21181 ttgtaagatt gtttcttcat ctttgatgac tttttctgcg ccattaacta atgtctcgta
    21241 aatcccttcg tagacacggc tgtaatcacc aacctcggaa attacttttt cttcatgata
    21301 actcccttgt tcatctatat aggtcaatac accataatgt tcgggcaaat cgataccaaa
    21361 gtcttcatga tcaggcatgt aaaacatttt cagatgttct tcttgtcgat cttttgtttg
    21421 tttcacgaac atcccttttt ttccgtaaac gacgaaactt ggacgttctt tgatgcggaa
    21481 ataactggat tttatcgaaa ttttcatatt tccatagaaa aaatccagat cgaagtaatc
    21541 attcattcgt cctttcccta aaagttgacg tacatcatag ctgatatcat ctggttcgcc
    21601 gaaataagaa agaacttgat ctactgtatg acaagcatgt ccatataaat agctgttcgc
    21661 agtagaaaat tcatgcgtat tttctggtac ttccggtctg aaataatcat aatgcatttc
    21721 tacttctaaa atatccccta attttccgct ttcgattact ttttggactg tcaagaagtc
    21781 agaatcgaac cgtctgtttt ggtaacattg gataaataga cctttttctt ttgctaatgc
    21841 aaataactct tctgcttcct ttgacgtttc tgcaaaaggt ttctctacta gtacattctt
    21901 accatgttcg agtgccattt ttccatattc ataatgaaat tgactaggtg tagtgatgac
    21961 aactaattgg atctcaggat catcccaaag atcagacagc tgatcagtat attcgatgtc
    22021 ttcgaatgtt ttccacactg gttcagatgg ggaaaaaatt tttttgatat tgattttgtt
    22081 cgtaagtttt cttgaaaaag gcaaatgata gcgatttgta ctctttccat tgccaagata
    22141 agcgattgta agcatgagtg gtgcctcctt ttattgatgt tttcagtata tcctagaaaa
    22201 gcgcagaaag tatttataat agctattctg gtaaaaaagt tcagaaaaac acttgttatc
    22261 attcggtttg gtgaaattaa tgttcaaagt aaggataaaa atcaaaaaga tagtagcgga
    22321 accaagtgtt tatcagtaag atagaatttg gaggtcagat catgcttatt gaagaaaaac
    22381 taacaaaaca agagttgttt accacgacag agaaacggat tgtcgattac atccgtagga
    22441 atatcgaagc tgcagtctac atgacgattg aagaattagc aaaagctacg tatacttccc
    22501 attcagctat cattcgttta tgcaaaaaaa tgggattcag cggatttaaa gaattccgtt
    22561 tcgagctggg gcgggaagtt catcaattga ttgctcagtt caatcaaacg attgatccta
    22621 attttccttt ttcaagtgaa gatagcactc agcaaattgc aaagaaaatg gcggatttat
    22681 cgatccagtc aataaaaaaa gcacaattgc agattgaaca tgaagattta gatcaaatcg
    22741 gtaaagtatt gaacaaggca cagcgaatct ttctttttgc aaaaggtgat tctcaaatca
    22801 cggcaaggaa attccagaat aaaatggtca agttgaataa gtttttgatc atggctgaag
    22861 aatacagtga ttcttcttgg aatgcagcca atttgactag cgaggattgc gccattttta
    22921 ttagctacag cggtcgcatc catcattatg aacgaataat gacctatttg aagcatgtag
    22981 aggcccctac cttgctgatt accggaaatc agcattcaga aatggccaaa caggctgata
    23041 tgtgtcttgt tatatcgcag gaggaatatg attttgctaa agtagcgact ttctcctctc
    23101 aaattgcctt cgattacgta ttaaatacgt tattctcagt gatatattct caaaattttg
    23161 aagagaatat ccgcaacttg aaaaacaaac aagaattgat gcaaaaaggc cttcttaaag
    23221 aatatcggaa aacttaagaa aagatcctca tttagtaata agaaagatag tgtttgatag
    23281 gctctctttg atatcatcat aaaaacaggc tgaagttttc tttttcaaac ctcagcctgt
    23341 ttgtttacgg aacgattcga atagatatat tatagaaatg aggaatagct aacttctgtt
    23401 tcttattttg aacgattgac gaaaaagata tataagaagg aggaaaatca gtatacatgc
    23461 agctaacaat ccatacagga aaaatcgttt gttctcggat actttctgta cttctttttg
    23521 aacttctttt gcatgataag gaacccggtg ccctctgaca agtaagcgat gactgttgac
    23581 catataaggt gtacaagtta ggagtgtcac gaaatcctgc cctttttcga ttctcagatc
    23641 ttttgtatca gtcggttcta ccgtcttgat ctgatcgact tgataagcga gtgtccgtcc
    23701 attgatttcg atatagaaag aatcacctaa agtcagtttc ggtaagtctg tgaacaactt
    23761 tgcttgaggc agtcctcgat ggctggaaat aacggcatgt gtattttctc cccccgtagg
    23821 ataagacgtt ccttccaata atgaggcacc tttttctagc agcacagtat tcgtttcatc
    23881 aaaaattggc aaatggacat tgatctcagg gatcgtcaaa ataccgatcg tatgtttctc
    23941 aaaataggtt ctatcctttt tcgcaggttc ttttttctta gtaaagggat ctgcacctgg
    24001 atttccacct tcttttgcta attgctggtt tttctttgtc atgttttctt ggattttagc
    24061 catgacttct tcattttctt tgaccgcttg ttgctgataa tgagagatga tctgctgatc
    24121 cagataatta ttcagcgcgt cgctaacaaa tggataagct agtatcccta ttcctgagag
    24181 aagcaagaaa agcatcagca gatctagaag tttccttttc tttggtttca tttgtgttct
    24241 ccttgtgatg actgtttgat aataaaagaa aaaggagcat ttgcgtaatc cgctgctcct
    24301 ttttccaatt cttgcaaaaa gactaaatct gactgtgctt gcgtctagca aagtacagtc
    24361 cagcaatcag tagaaggact actcctgcac cgatatacac atagattccc ttaccgcctg
    24421 ttgaaggaag tgtacctttg tgtttatttg gtattttttc aggagaaatc aactgtcctg
    24481 ctgttacata agattgttca tcgatagtaa atgctacacg gtttgttaat ggaacatatt
    24541 tttctggcgc tttcgtttct tccagatagt acgtgccata ttttagacct gtcacatcga
    24601 ttaaaccgtt acttgtggtt gtaaaaaccg ttgctgattc tttcgccgat acccagctga
    24661 cggcttttgt ggatgggtcg atcgataaat atttcgctgt gtcactatct tgatcacgaa
    24721 cgacgaattc tgctccagca agtgtttggt ctgatgtaac gtcaccatct actttaacaa
    24781 atcgtttgcc cccagtaacg acatcgactg acggtggtgt ttgatcattt gtatgcccgt
    24841 tatcgacatt tgcttggtta gaaaatcctt tggttggatc tgctttttcg ttcaaatgca
    24901 tatagtaaac gaatttcaat gtaccgccag gagttaatga aggaatataa ttcggatcaa
    24961 ctgaaaccgt gaatccgtct gtttgctcag tgacagaata atttactggg tcgatttctt
    25021 tatttccatc atataaggca taagcatacg ttcctgaaga atcattatca aatgttaaag
    25081 cagcgtcatg agtatcgata agtttgaatg ttgtgtactt gttttgcgtt ccttctttat
    25141 cagcgatacc taatgggata ttgacagaaa tctcatattg aattttttca ccgattgcga
    25201 catcttttcc attcaattga ggtgttgttt tatcaacttt agacgtatca tttttgatgg
    25261 tcttttctac tggtgtttgg ctatttgcca ggatctcaaa aggcgtgatt gtttgctttt
    25321 caatcatttc cgcattatca ggagctttta cttcttctaa attatatttt ccaacttcta
    25381 aatgattaac gatcaactgg cctttttcaa tagatgcctc ggcaaagtca ttgttgccga
    25441 tgtcataaga atgaccagta atgaaatgtt tggctttggt ttgatcagtt gtccaagtgt
    25501 acaatccatc tgtgacactt tggatgtatt ttttgacgct tggtgttcct tcttctttag
    25561 aaataataaa ttctgctcca tttagtgctt cgttttcggc agtaccgatt tttgtaactt
    25621 tcaacgttcc atcattaccg actgtatttt tagggtagag atggatagta tctagttctt
    25681 ctgtcccgta tttataagag ccatctgctt gtttgatcat ctcatataca gggaaagcta
    25741 aaaccatgtt tgcggcagct gacactccgt cttttggttc ttctttgatc gtgtagactg
    25801 catctttccc attttgtttt ttaggtaaag ataaagtgac atttccatca gcatctgtcg
    25861 ttcctgaagc aactggtgta ccaggagtca aagactggac tgcttgtttt gctgcatcca
    25921 cggacgctcc tttatctcgt tgcgcataaa attcttgagt gacgttataa actgaaaatg
    25981 aaatatcggc taatccttgg tattgatcga attcgctcat ttctttcccg ctgttttgga
    26041 ttaaaggatc gggtaaatca gtcattttct ttttatgaag gatgacttga gctgctgtct
    26101 cctctgcaaa cacttttttt gcgccgaagc tgtttgtgag taatggtaaa ataaggaaaa
    26161 ggactcctaa cataacgttt atttttttat gatttttcat tttttcactc ctgttctttt
    26221 tttgataaac aaccagattg caaagcttgc gaataccata ccaacaacgc ctaaaagtgt
    26281 atttttcgtt tcgtttgttt ttggaagaaa tggacggcta ttttcttttt tattgactac
    26341 tttcaatata ctgtccgcct ctttatcctc agaaagaaaa gttgaaatgg taaacggaat
    26401 ttctgtttca cttaaaatat aaccagaagg tgcttcgatc tcttttaatc gataacttcc
    26461 tgttttcaac ccggaaattg aaaaagcgcc atttttatcg gaaatcaggt gaagcgcatc
    26521 tgattcgttc tttgtccact tgtatccgtt ggctgtttgc tggaggtaat ttcctgcttt
    26581 gtttttaata aggaatactg cgccagctaa tcgtttatct ggtttatttg tctcgatttt
    26641 gacaaattgc ttactccctg tatatacaga gactttcttt gtactgacac caaaaccaaa
    26701 atccagtgtt ccgttgttgt tgatttcctt gttcgcctgt gctgtactgc ttagctgcat
    26761 ctgataagaa atggtcagtt tttttccagc atatttttcc aacgaaggga tcgaaaataa
    26821 aacatcaaat ccgtgaggtt tcttgtgtat gacaaagcct tctgtcagct tttctccatt
    26881 cgatgaaatc gttaaactat taggtaaaaa cgttaatgct tcatccgcac tatctgacaa
    26941 ttcgaacttt tgatagtcaa ggatatttac aggaattgtc gtatgcaagg aataagtgat
    27001 tttttcacca atcgtaaaat catttctagg ctcttctaat accttctcaa aaggtggatc
    27061 agggtagtcg ttttcttcat ttttaggata caaatggata gttgaaagtt tttgattgtt
    27121 ttgtccatgt acaggaagaa caactaccat attctctgcc ttttctttga cgacttctgg
    27181 tactttggat tcaatgaaaa gatacgcttt atctcttttt tcttgatctt tagcggccag
    27241 agaaaaagaa gcgataccat cttcgttgtt aagagttgta gttgttgctt ctgcggtaaa
    27301 cataccggaa gacgcaccgt tttttgcgat ctctgcttgt gcttcttcta ccgttttgcc
    27361 cttttcccgt agatggtaaa aagaatctgt gacatcataa acttggaatg tgacaccatt
    27421 taatcctcga tacgtttgta ataaagcttt ttcttgtccg tcatttggat gatttttcgg
    27481 cagttgcccg tttgggaaaa gcaatttgtg taaagtaatc tgaaccattt ctgtttctgt
    27541 ttctgttgct acctgagtaa aagctggttt aaatagtaac aagaagagac acatactaag
    27601 ccaaccaagt tttttcatat ttagctgccc cctctttctt ttttcaaaaa aagataccaa
    27661 atagaaaacg cacaaccaat catccctact agatagattc ctaaacggcc aattcctccc
    27721 gtttcaggta atggtacttt tgcctgattc gtgatgtcta aagaaatctg gttgtttttg
    27781 gctcctggtg acagaacaga ttcatgatct tgtccatcca cttgaattga cccatcttcg
    27841 tgtataacta tagtaactgg ctcttttaga ccttggtatc cttctggtgt aaaagtttcg
    27901 gtcagcgtat aagttccagg ttttaaattt tcgaatagaa aggtatctgt ttcttgtcca
    27961 tcttttggcg attctaagtc catttctggt ccctgcagtc tgaattttgc tccttttaat
    28021 gtctgtccgt tgtcttcttt tttgtgtacc gttagatcaa acgcttttag tcgattttga
    28081 tgagtgagcg tccatacatt atttgtcatc tcggtatttt ttcccgtgta aatgattttt
    28141 ccatatttat cgatttggaa ttcataattt cccggcgaag tatcatatcc ggcaggacca
    28201 gctgtttcaa cgactcgata tttaccaggt gtgagtgaat caaaactggc aagacctttt
    28261 tcattcgttg tttggctgtc aattggctga taagtaccat ttgcttcttt tttctgtagg
    28321 gaaaatgctg ctcctttaag atttatctct ttgtccgtcc cttgcatcgc atacttacgt
    28381 actgctacag gaacttcaac aaaaggattt tcaatagtca actggatcgt atcatcggaa
    28441 gtagtgactg tttttccatc aatggttacc gtaccatcag aagcgatctt gatttcccaa
    28501 gtagtctttt tgcttaatcc atgcccttct ggagcttttg tttctgtcag cgtataggtc
    28561 atttcttttt gcaatttgac attttctgga agagaatagg ttccgtcgcc atgatctgtt
    28621 agtaaagtat caatagaatc tcctgttaat ttgaaaacag cgccaataag atccttttca
    28681 cctgtagagg aatttttcgt tattttcaag tttaacggtg tgaattgttt agtatttttc
    28741 catgtcattg catctatttg ttgagaatcg tatcctggta caggtaattc tttgattgtt
    28801 tggtaactga atgcttgacc ttgattattg tattgaggta gtgataatat ctcttgataa
    28861 ctttctccgc tatttgcaga taatttgtca atgtctgcac gttcccacgt atttgttgta
    28921 tctttagctg gtttaatgat tcgaatatat ccgtttttcc aagctgcagc atttgtcgta
    28981 tgttcccgtt gaatctcaaa agtaacttga tctggacgat cagctagatt gttgtcaaat
    29041 tcttcccata actttttgat gtgaagactg actccgggag ctttagcaga tggtataccg
    29101 aattcagcta attcattggt atcaatgttt ggctggaaag ttgtcctgcc gttcatttga
    29161 taccagaaat ttggatggaa gtcctcattt tctgtttgga ttctcacttg gtaatggatt
    29221 tggatttctt ggtcttttcc taaatagatc tgattgctct tgatggtatt tccttctatg
    29281 gaaatagatg gagtgaccgt tgtaggactt gtccccacac tcttgactga aagtgtacca
    29341 ggctgataaa tgaatggttc tgcgattgga tcatttattt gtccattgct tacagttgct
    29401 gagatctgta cagctttttt ggctaggtat tcagagatat ctgttgcatg atcagctgat
    29461 tcatagtaaa gatcgccttt ttcatctgat gaaaccattt gtcgcatacg agactctact
    29521 tctgcttttg agagaccagc tgccggatcg ctttgaagtt ggatgccaag accatgtatt
    29581 tcgattcctc gttctttgag tgccatcgct tctccgatgg ttgcgataaa cgtactgtcg
    29641 attcttctgg atagattgtt ttggtcaggt gcatcataga ttcttgagat tagagaagta
    29701 tttcccggcc gatcttgcgt attagaaaac tgagttccgt aataattgct gcttgattgt
    29761 gcgtgtaccc gctgtacttt ataggaaaat gttggtacac catccgtcag caaaacgatc
    29821 acttttttat gtccatttgg aacggatagc atgcttcctg catctcttag tgctttttgt
    29881 gtaaaagtac caccatttgt ccgtgaaggt gtaatggatt ttacttgatt tttcactgaa
    29941 tcaaatgaac ccatctgtac tgcaccgtta ctgtagctat atccttcgct tgagtagccg
    30001 acatatccca tattgatttt gtctgtgata ccgctatctg ctaaagtatc gacaaaacga
    30061 tcgacaccaa tctttacttc accgatccga ttattgtcgt tcatacttcc tgaccagtct
    30121 acgaccaata cgagatcaag aggcgtgata tctttttgta catttccacg agcattcaaa
    30181 taaacatcaa acaatccggg tgtacttgtt tcctttgcat actttcgtat cgcatagtct
    30241 gcttgattac cggttcctcc atattcgata tacgaatggg tccgatcatt aggatctcca
    30301 ttccaagaat ttataccatc ccattgattt gttccgtttt tattgccttg atgatcaaga
    30361 acatttgtat ttcctgtagg ttgccaactg gctttaggat aagtgccctg atcatctgtt
    30421 gtatattctg taggaatcgt ttgattagat ggcacactca cagctggcaa ggataatgta
    30481 ggcaatgtaa atgggctgac agaattttcg ttttcttttg atgattcttc ttctatttcg
    30541 tctggctttg gtactgacac atctgtatct ttcttatttt ctgtctcatc aataatgtca
    30601 tattctttat tatcgacgat cagcttgttt gcctggctgt ggtaaagtgc tggatcgatt
    30661 ggaaaactca gatcgatagt ttgtttagtg tttgatgaga tttccaacgt aattttcccg
    30721 ctatttacct gataagcata gccatctcct tcttttcttt ccacatcact taagtaattt
    30781 tcgactagac caatttcttt ttctatcttt tcagcactgg tgttattcgc atttagcttg
    30841 atgttcagtg ctttttttcc agactgatcc gaaatgactt cagctgaaat tgtttctaaa
    30901 actgttggat catctgtagt gtctgctatt gcagataatg gcacaaagtt tgataaactc
    30961 aatattatca gcatgaaaat aactttcagt ttcttccctg tggtcgtcat ttttcccctc
    31021 ctttttcttt ttttctgttt tgacgtgaat agtgaaaatt attaaaataa acaggcgtta
    31081 cataatttta tttattttaa tatgataatt attatacgaa gggcgctaaa gggaatcaca
    31141 ccaattttca taccaattgg tttcatttat gaagttgatt ccttcgatta atgagcgttt
    31201 cattgaaaga aaagggttga gggagaaaaa catatgatca ggttgttgga attaaactat
    31261 agaaagatgg aaaaaatcat tcgaattctg tccatttctg acaagtggat gactaaaaaa
    31321 gaacttgcca aacgaattgg tagttcagaa tctacatttg taaggtatat cgaagagatc
    31381 aaaatccgct ggggaacgga tgtaacgatt caatcttcac aaaaattggg gtatcgtctt
    31441 gaacatttga atgtaccagc ttatttaaat gttctgatgg aaatgtcgca aagttctaca
    31501 aacagtcgtt tattaaatga gattattgtc aatccgggta aaacgattga gtattattgt
    31561 gaaaaaattt ccatcagccg gtcttctttt acacgtaagt tgaaacaatg caatcaaatt
    31621 ttagaaagct atttattaaa agtgatcgtt gaccaaggtt ttcagttaac tagccttaaa
    31681 agtgagctcc agttgcgtat ctttgttacc tttttctttt taagttacta cggaagacgc
    31741 acgttgccat ttccaatcga aaaaaagaag atcaaagaaa ttatggtcaa gaataattgt
    31801 cagatgaatt tgtttcagga agaaagccca tatgagcaat ctttctttat tatgtatttt
    31861 gttgtgcatc tcatgagaga atcccaagga actccatttg aatggaaagc tggagactct
    31921 tatgtacagt tgataaatat cgataaaaaa gacttcttaa tcttgaaaaa tctcttagga
    31981 aaattgaagt acagcagcta tataaaagta gtgaactatt ttgtcaatac gattttttca
    32041 gaattttcgg agaaagaaaa aaatgaaatc agtgagcaga tacgaaaaaa tatgtcctac
    32101 tgttcgtttt catccgctga ttgcaaagaa aaaaacgaag aatatgttgt tcaattatta
    32161 acgcaaattt attttttctc ccgtattttc ccatacgata cgactcattt gacgatccgt
    32221 gcaacttact ttgcacagca gatgcaacac cgccagatac gtctatatgc tggatttcgt
    32281 gcagaacttc aattttattc cactctactg aatcagaatc tgatttatta tttatctagc
    32341 tatatctttt ggatgcttat cggtaatcct gaaattcacc attatacttc tgataaaaaa
    32401 atactgatta tcagcgactt aagtcttcga catagtcaat atattgaaga atatatcagt
    32461 gatattcttg ctgtgcataa aattcactct gagacaactg ctattactga agatcagctg
    32521 tcgaaataca atcttctcga gtatgatctg atcgttacga atcaacccat tttgaatgcc
    32581 catgttcctc atatattgat tgatgatagt gtgtcattcg caaatgaaga agaactcatc
    32641 cgcttattcg agttatgaat aatcaaaaaa cagctatgta taaaccaccc tgataaaaat
    32701 ttcaggacaa atacacatag ctgtttcttt ttattgtatt ccttcagcaa tccaacgaga
    32761 tttatagcga aagtctttta acaactcttc ttttgtccaa ttatttgcaa tgagatccgt
    32821 caaagtgttc attacgaggg tcatggaaaa gtataggaaa ctaactgctt cttccgacga
    32881 ttgaatggag aaatgattga ttcttaataa ttccagccat ttttcgttca ttggagaatc
    32941 agccgatagt tctctacgtt tggttgtctg ctgatcattt cgctgggtta acaagtataa
    33001 cctttgccaa tagatacttt cgtgatctaa gtcgcagcac cactctaaat atttttcgat
    33061 ccccaaaaag aaatgttgtt tgttttcgct aatgaatttt aagatatctt gatggactat
    33121 gtgagaactg cttttgatcg tatacaaata agcatcttcc aaatcttgaa aatatttata
    33181 aaaagcaccg cgcgacatcc ccatcttgtc aacgatttct gagactttga cttggctgat
    33241 gtgctgtgag caaaatatct ccagtaatag gtcatcgatt ctctgtttct ttgcgttcga
    33301 taaattgaaa aaagtttctt ttggcatcca catctctcct ttatctctga ttatacgtgg
    33361 atagcccttc attgtcatga aggattcatt ccttcttttt ctcttcttct gtcaagtacc
    33421 catcttccat tcgataaacg cgatcactcc attgaatcat acggacatca tgagtaacca
    33481 taatagtcgc tttttgtttc tcatgagctt ctttaactag taatttgacg acttcatatg
    33541 catgatctgt atccaaacta gctgttggtt cgtctgctaa gatcaagctt ggctcattat
    33601 ataaagcgcg agcaattgcc actctctgtc tttctccacc agaaaggtct ttaggataac
    33661 tatttttaag tgactggata tctaaagatt gcagcaagga atcgattttt gttgaatctt
    33721 ctttttttga agtaacttgg tcaactagtt caaattgttc ctttaccgtt aaaaaaggaa
    33781 tcaaattaga gctttggaga ataaatccta tttctttaaa gcgtaaatct gctcgttttt
    33841 tttctggaag ctcagttagt gagtgaccgt tgatcactat ttctccggaa gtcggtgttt
    33901 gcaatcctcc tgaaatggta aggaaagtac ttttacctga accagaagga ccaatgatgc
    33961 tgacgaattc tccttccttt acataaaaat caattgcttt caaagctgag acctttgtat
    34021 gaccagaacc aaatgttttg gtaacttttt tcatcgttaa tatttttttc atttttatta
    34081 acctcctatt gctgtaattg ggtctacttt ggttatcgta cgtatcgaaa acaatccgcc
    34141 tagcattgcg acaatgatca aaatcccact atatagcaac cattgtgacc aaaaaacagc
    34201 aaaaggcatc gcgcttggta aaatcagact agtcaaataa gcaaacaaaa gagcaagcag
    34261 aacgcctacc accccaacaa taaaggactg tgctactaaa gatttagcga tgaagaaatt
    34321 tttgatccct tgtgctttca ttactccaaa aatagcagtc ttctgaagtg tcatcacgta
    34381 catgaaaata ccgaccacgg cagcaaccac taagaacaaa aagtatatca tcgcatttaa
    34441 ggtcatattt tgggcagaat agccaggaat actttcaatg aatgtcccaa tagttaataa
    34501 ctgtagaccc ttcgatgctt tttctttcga aattttagct gaatccttta ttacgatccc
    34561 attgatcggc tgttcttctt cagaagcaaa cggctgttgt ccatacttca aagcagtcca
    34621 cgtatctagg gacgcataga tgacgggaga aactgtgtaa taagtttcag gaaagatacc
    34681 gacaatcgtc aaaggatcgt cgtaactgcc aattttgatc gtatctccaa ttttatagcc
    34741 tgaatcagcc agattttgag agatgatgat ttcattcttt gttttaaatt catgcccttt
    34801 cgtgagtttt ggtaataaaa atgcttcttt cgttgtgccg aaaactgtaa cattttcttt
    34861 gttttttcct ttgattgctc cgctgtacaa tccgattggt gctttctctc ctccttcaat
    34921 gtatttcaga tctcctcttg ttaattgtga agctgcaaat acttgatttg catcctccga
    34981 caaaacaatc tcttgtgcgt cccaatcatc gattgctttt ttaaactctt ctgaaagtcc
    35041 attcgctagt cctgacaaca tgaatacgac atacgcaatc aagaacataa taccgacgat
    35101 caagccatat cgtaatttag aatagcgaat ttctttgata gctagaaaca tttgtttttc
    35161 ctccctatga tagattgata gtgacaaaat gtcacttgtt gaatagtgta actctaaagt
    35221 gacaatttgt cacttgtgtt gtttttttct tatcaataaa ttgtctaaga catctttcga
    35281 taaaacctta acatgagtca taaaaaattt atttaagcct tatttatgct ttccatgata
    35341 aactaaagtg accaaatcaa tgaggtggga aaatgattaa actattagta gtagaagacg
    35401 ataaagtatt atccaataat ataaaagaaa tactcgctgc aatcggcgag ataacacaag
    35461 tttatgatgg tagtgaagga ctgttcgagt tggaaagtgg agtttatgat cttgcaatct
    35521 tggatttgat gatgccggaa atgaatggat atgaagtgtt agcaaaaata cggaaagaaa
    35581 aaatacagac tccagtattg attttgactg caaaagatag tttggaagat aagattgaag
    35641 gatttgaacg aggtgcagat gattatctaa caaaaccttt ctatcgagaa gaactgatca
    35701 tgcgcgtgaa agcattattg aaacgctcgc taggtctctt tgatgaaaat gttctgaagt
    35761 ataaggatat cagttgtgat ttaaaaagca atgaagtctt gtataaagaa caagttttgc
    35821 ccatacaagg aaaagagttt gaattgctcg tttatttttt acaaaataag ggaatcattt
    35881 tgacaaaaga gcagatattt gaccggatct ggggatttga ttctgatacg acgattacag
    35941 ttattgaagt ttatatgagc aacttacgaa aacatctgcg aagtactgga ttggataaag
    36001 aaatcaaaac cttacgtaat gtcggttata tactgcaaag ggtgtgatgc aatgaatgga
    36061 gatattacac gaaagcaacg tagtcgcttt tttgcgacaa atgtagcagc atttgctttt
    36121 atcttttttc ttttgggatt cattacttta cagatattaa accaatctgc atacagagaa
    36181 acagatctgt cattagagaa aataaatttt gattctcgaa cagtacaatt agagatacaa
    36241 agatatcagc aaggcgatcc ctttttaaac ccgactcaaa cggaacaaaa cagaacaaac
    36301 atggaacagc cattaatgga accatcaaat gaccgtttta acactcaagt gatcttgtgg
    36361 tctaaaaatg gtgagatatt gaataaagca gctattggtg ggcgtttcaa ccaaatcaaa
    36421 gaattaaaaa tggatacgaa aaatctgaat accatccaaa atattgaatt aaatgaagac
    36481 agtggctcat ctgccctctc tttccgttcg attacgaaaa aagcggagac gaatcaagat
    36541 ggcgttgcct acatccaaat tttagaaaat acaaatcaaa tcaagaattc attagcgact
    36601 tttcgcacga ttatgatttt gtgtatgatt gttttttgga ttctctctat tggtatcagt
    36661 tattacttgt ctagtctaag catgcgccct attattcttt cttggagaag acaaaaagaa
    36721 tttgttgaga atgcttctca tgaattgcgc acaccgttaa cgattattca aaatagtttg
    36781 gaacatcttt ttaccaagcc taatcatact attattgaag aatccgaaag catcgctcaa
    36841 gcattgtcag agacgaggcg cctcactgga ttgacttcgg atttgctgac aatcgctcgg
    36901 aatgattctg atcagcagct gctttcaaag caaatgatca atacacaaga atatatcgaa
    36961 caacttgtta aaccatttca agagatggcc atcatagatg gaaaagagtt tattctccat
    37021 aacgatgcaa cattagaagt accaattgat gaaaaaaaaa tccatcaagt tttggttatt
    37081 ttgctggata atgcattaaa atatacgaat ccagaagata agatcatttt ggattcatct
    37141 gttaaccata aagaatggct gattcacgta aagaatactg gcccttctat cagtgaagaa
    37201 gatcaagcaa ggatttttga tcgattttat cgggaagatc aatctcgggc gaaagagact
    37261 ggcggatacg ggcttggctt ggcaattgca aaacaggtaa tcgaacagca tcatggacag
    37321 atttctgtat ccaattatct tccacaagga gtcgatttta aagtccaatt gccgttgaag
    37381 cctaaaaaat aatgcttatc cctaaaaaag ggctgtgaca aaaatcttgt cacagccctt
    37441 ttttgttgcg aataaacggt gttccagaag tagcttcttc ggaaataagc cgaaatttct
    37501 ttaagctgac cacttctgtc acaacctctt tagcttacgc ttacgatttt ttattgcggt
    37561 cttcaatgtg tacatcaccg cttgtgtcaa tatccaattc ttctcggcta acatcttctg
    37621 tcactttttc aacatcttca tgttcttctt tatgaatccc aacttcttca cgaataactg
    37681 tatgtttgct tacatctact tgctcttctt tgattgggat agtaatcgtg tcatcttcca
    37741 atttttcgtc tgttccctgt gaagattgat ctgttactgg tttgcgttcg atgacgattt
    37801 cttcacgttt gaccggtact tctaccgttt cagtatcgtt gaccacatgt ttgtggatgt
    37861 cgacttctcc agttgtcaca tcatgtgtat ttacatctaa tttttcttct tttagttgaa
    37921 ttgtatcatc atctagattt ggtttgcgat cgatgtcttc agtcttgctt gtacccattg
    37981 tatcagtctc gccaggtacg tccatcggat cagttcctgg tgtcatattc aatggatccg
    38041 ttgtattttg aaagcctgtc gtgccaccta cgtccattgg atctgttcca gtgccagttt
    38101 ctaatccagt ccccattgtg cttgctgggc taacagtatc ttgtgtatcc attgtatctt
    38161 ctgttgtttc tggctgacga tagcctttta ctgtgatgac aaattttcca tcagaaatat
    38221 ctcctctata ttgatataaa ggatcatttt ctggtgtagc cgtctcagaa tcataggtat
    38281 caaaagaaaa agtatctttt attttttccc ataaagaacg gtcttcttca ccatccatcc
    38341 gattttcatc tgtttcaaca tctataccag acaatccgtc aaatcctagt ctagatgctg
    38401 cttcatcagt agtataaata acgatatcat ctcgttcata cccttcgtta cgcaaccgtt
    38461 caattactga taatgcttct tccctcgtac tatagcttcc gatgaccgtt aaatttttct
    38521 cttccatgta aattcctcct agcaaataga tttatgattg gtatattgtt acttataaat
    38581 ttattctata ggagacgatt gttttctaca aatatttaga ttggaatttg attaaaacaa
    38641 tttaattgaa atttgaaaac atttatgtta atgaaagtca tattctaata aaatgtcatt
    38701 tgtttctata tgaattttta tgaaaaattt catataattt ccactcgttg tgatggttta
    38761 atttatccaa tccatcaata agcaaacaac ttatataaaa aaggattaat tctcaatata
    38821 attgctccta ccatagaata aatcttcatg taaagtaatg aagtaccaac tatttctaat
    38881 acttctttaa gaattatccc aacaatatcg ttagacaaac cattattgaa taaatggaga
    38941 gccaatcgat tctgaaaaaa tgttttctaa tttttcggaa ataaaagaaa gagcctcctg
    39001 ccattagaac gaaccaaatc catttcattg ctcatgaagt aaccacatag ctaaaaagag
    39061 gataaatcaa ttaaaagatc aaaagaatga ctgcactaat aaaataacta aaaatgtatc
    39121 ttcttacttc atatctttta gctagtactt agttaaaaat ttgcttccta agatcaagac
    39181 aaactgtcta tcttcatttg atgaaatgcg gtaaaatata gaaaatgaac actattggag
    39241 ggaatttgta atggacagca aaactagaaa acaactgcaa gaaaagttaa agcaacgtac
    39301 acacttgctc ccttccgtct ttcaacagtt tttagaagaa aatcgttctt ccctttcatt
    39361 aggcagtcgc tatgaatacg caaaagattt tcatctgttt actgattatt tgagaaaagt
    39421 attttctgaa gaagtggatg atcagctgat tatccattta gaaaaacaag attatcagaa
    39481 tttcttagct aaaatctatc gacatactcg ttcgttccaa actacagcga accaagaaac
    39541 ggagcaattg tttgaaaata gctattacgg gatttcaagg aagcaatatt cattaaatca
    39601 ttttttacga tttttgtatc aaaaaggact gattgaagaa gagatttctc ttttgggtac
    39661 aagtttaccg gaaacaacca aagcagctcc gcgatatatc gatgcagaac ttttcaaaga
    39721 ttttgagcat ttgtttatcc cgattgaagg ctttcaaaca agcagagaag ctagctttga
    39781 agaaaagaat agaccgagga accttgcaat tactgccatt cttctgtatt gcggattaaa
    39841 gatccatgaa gtagtagaac ttaaacgtaa agatgtatct ttgaccaagc agattcttaa
    39901 tctgaataga aaagaaaaca gactgaataa agtaccaata ccaaaagaag cgatgccatt
    39961 tctggagaac tattttaagt taacagcaca aaatacggac gaaaattctt ttgtttttcg
    40021 ttcctcacat gatcagcaaa tttctcctcg gactattcgc caaattttga gcaaaattac
    40081 agtatataaa aacgtatcgc cagaactatg cagaaaaaca tatcgttatt atgcagaact
    40141 atatttagat gatgctgacg cggtaaatta tttatgtggt aatcgatacc tctcatccca
    40201 ttcttttcaa gatatctggg aaaagatggt tgatttttct tatcacgaat tagcggcatt
    40261 cgttcgggtt tcttccatat aaaacaaatg tatgatcttt ttttcacaat aaactatgtt
    40321 attatataaa ggattatgtt acgattatct tgaatttgta tattacttaa caaaagataa
    40381 tggagtgtga atgatggaga ccataacgta ttcccctttg aacttattta cgaattttca
    40441 agaagctgca gctaactttt ccgagacccc gattatcttt gatgagccac tttcagcttt
    40501 cccagaatta ggacttgaaa caacttacaa aaaatgctgc gaaagtgtgt tgaaacgggc
    40561 ttatcaattg gctcatcttg gagtgaaatc cggagacaaa gttattattt ataagagttc
    40621 cgcttttgac acataccttc tagctgtttc tgtgtcttac cttggtgcag tgcctgtcat
    40681 gacttcttat catctaccaa caacaacaat ggaagtattt atcgatcgtt tagaagaccc
    40741 tttcatttta tttgatgatg agacaaaaaa gcgagtgaga gaaatttcaa atggaacaaa
    40801 atcaaaacag attcctattc tttatttaat ggaacaacct gctttgtctg tttctcaatc
    40861 atttttagac aaaaatgaaa tttcttatat gacccatact tctggtacaa ctggtatccc
    40921 taaacttatt tgtcattctg ctcattctat gggatggcgg acgaaatggc aaaaaacagt
    40981 tttcacaaaa atttcggaaa agaaattaat cgcttttcac atctctccag tgcattccag
    41041 attcaatatt ggtatctctt ctttgatgtc catgggattt cctatgatgc ctttagcaaa
    41101 cgcacaaagt tcaaaagttg ttcatatgtt ggaagctcac cggccaattg cactagagac
    41161 ccatccgaat aactttgttc agtggcgttt tactgctaag gagcatccag aagctttttc
    41221 gggtatccga tactatcact ccacttttga tgctataaac aaccagacaa tgaagacatt
    41281 tttgcgaacg tcgatggcac aggatgcggt atttcttcaa gtatatggcc aaagtgaatg
    41341 cggaccaatg attcttaagg ctcatacact agagagtttg aaaacaagtg atgcaagaga
    41401 tatgggcgtt ggactggaag acatgacaag cgctcggatt acagattcag ttgggaatgt
    41461 cctacctgaa aacacagatg gtcatataca attactgtct aaaggacggg ctttgactta
    41521 ttacaaggaa gatgcacggt tccaggaaaa tgtttatggc gattggtggg atagcggaga
    41581 ttacggtttt atggataagc atggccacct atttttgaaa gatcggcaag tggatttgat
    41641 cgaaacgatc aatagtacat tagctattga agacttttta ttagattcgt tagatttttt
    41701 ggcagaagta gtgattgtaa gggataaaaa tcattctcca caaccgatta ttgctttagc
    41761 tcctgataaa gaaatggatt ggaatcgatg gtgggagcaa gtccatgaac ttcctcgctt
    41821 gaacgtccct atcatccgtg attttgatgc aatcccgcga acagctacaa tgaaagtgca
    41881 acggcttcaa atcgagaaag aattaaaatc acaaaagtcc taactattta aggaaagaaa
    41941 gatccatttt ggtctttctt ttctttttgt ttttttaaat tggatgagat aaaaatatgc
    42001 caattggctg tttttctttt ttgaaaattt cgttatactc ttattcatgg aggaaaatgt
    42061 tatgaaaaga atcttgacaa ttggcggttc tgatccattt gctggtggag gaatccaaac
    42121 agatttaaaa acttttgaga attttggtct attcggacta agcgccttga cttctatagg
    42181 ggcactagat attaatgagc gatttatgct ggaatctata tctttggaat tgcttaaacg
    42241 acagttagtt tcgattgata aaatgacttc attagatggt atcaaaattg ggttattgaa
    42301 ttcaagtgaa gcaatcatgg ttgttcgaga cttcataaaa acaaaacaaa atatcccaat
    42361 cattttagac cctgttttgg cattcaaaga aactgaagct gtgatcaatc aattgtatat
    42421 ttcgcagctc atcgaagaac tttttccact tgttacgatg attacgccta atttggcaga
    42481 agctgcttta ctttctggtc aagctcttcc ctcttcgtta acagaaatga tccatctcgc
    42541 taaagaactc actgcttcag gaacaaaaaa cattgtgatc aaaggcggca caaggatggc
    42601 tggagatgaa gctgtggatc ttctctattc aattgatcat tatgaggtat tttctcataa
    42661 aaaaatcgac actacaacag taaacggggc cggttgtgcc ttttcttctg caataacagc
    42721 gaatcttgtg ttaggggcag attttaccaa agcagtagca gaaagtaaaa aatttgttgc
    42781 ccattgtatt tctaatggcg tgctgatgaa cgatcatacc ggaagtgtct ggtctggcgg
    42841 tattcaaaaa ggaggaatga gaaatgaaaa ctaagcaatt aacgatctat gctatgctga
    42901 ttgcgttgac agttgccctt tcattaacaa ttttgatccc tgtaccggct acgaatggat
    42961 ttgtgacatt atgcgaggcc gggatctata ccgctgcttt tctttatggt ccaacgggtg
    43021 gattagttgt tggtgcttcc agtggtctat tgatcgattt gatttcagga tatccgcagt
    43081 gggcagtttt ttcattagtg atccacgggt tacaaggata tgttgctggt tctttcagca
    43141 aatcttctac tcgtatgtgg ggattagggc ttttattagg aagcttagtt atgatcatcg
    43201 gctatggtat cgccggttgg attttatatg attggccggc tggagtggct tctataccag
    43261 gaaacatctt ccaaaatctg attggaatgg gacttgcctt tcctataact gcaagtcttc
    43321 aacgtattca agcacggaag caatattaat tgaaaaagaa ggttttagaa aatgaagttg
    43381 tcagaaaaag aattaaaaga ccaattgact gaaatagtta atgacatatt ggctgaggca
    43441 cacttgaaaa aaggtgatct ttttgtttta ggatgcagca caagcgaagt cgttggagga
    43501 catattggaa aaaattccag tgcggaagtt ggtcaatgga tcatccgtac gttaaaagag
    43561 ttgttggatc ctaaagaaat tgcgctagct gtccaaggtt gtgaacattt gaaccgagca
    43621 ttagtagtag aacgaacagt ggcagaagca aaaaatttcg aaattgtctc tgttgttcct
    43681 gcactacatg ctggaggtgc ttgttctgtt gctgcttttg accagttcaa tgatcctgtc
    43741 gaagtggaac acgtagttgg acaagccggt atcgatatcg gtgacacatc aatcggtatg
    43801 catatcaagc atgtccaagt acctgtcaga cctcgtttga aaacccttgg acaagctcac
    43861 gtgacagccc ttcgctctcg tcctaaatat atcggtggtc ctagagcgaa ttattgaaat
    43921 gagtaattga cagagcaatc tgaaaaactt cctcgcaaag tccaactaat gaactttaac
    43981 gaggaagttt tacttgtttg ttttatataa ccgagcttca taaggtgcta gtatgccttt
    44041 agcttcaaat tctccgctag aggaatttga caagagtaat gtccaggact gatctgcttc
    44101 ttttggaagt tcatagtcag caagtgattc tgttaagttt accataataa gatattgttc
    44161 atcttctaaa tatctttcat agacataaag ttgtggatgt tcatgaagat attctttata
    44221 agaaccgtat atcagacctt tattggtttg tctaatgcgg atcatgttct tatagaattg
    44281 caggaccgac tctggttcat attcttgttg ttctacattg atcgttttgg tatttggatt
    44341 agtgatcagc caaggttcat gtgtagaaaa accagcatat tgttccgaag tccattgcat
    44401 gggtgtcctg ctgttgtctc tcgttgtttc acgaatgata tccaatgctt cttctcttgt
    44461 cttaccttcc gcaagcagcc ttttataaag tcttttcgaa tctactgcat caacttgatc
    44521 gatcgattca aagggcatat tcgtcattcc gatttcctgt ccttgataaa taaatggcgt
    44581 accttgcaaa aagaaataca tcatagcgat tgcttttgca gacatagccc aaaaatcggg
    44641 acgagtatct ccaaacactg aaacggcacg aggaacatca tggttttcca tatataaagc
    44701 gttccatccg ataccatcca atgaagtttg ccaagcgctt aatgcatgtt tcaatttaag
    44761 gacatctaat tgcccttctt tttcttgctg ccagatatgg atatgatcaa attcgaatat
    44821 catatcgaaa taaccgtctt tccctaccca ttgtggtcct tcttctgcag tgacaccact
    44881 tgcttctccg acagtcatga tatcgtactc ttcaaaaaca tgtttcaaat cggtcaaatg
    44941 ttcttcgatt cctgagacat tttgaaaggg tgagaaaggg ttctctgtgg cttttactga
    45001 atattcatct ttttttacat gagaaatcgc atctaaacga aatccatcta tccctttatc
    45061 caaccaccat cggatcatat tgaatagttc ttctttcaat ttctcgcttt cccaatttaa
    45121 gtctggttgt tcttttgcaa aaacatgcag ataatactgt ccagtttcat gagaatactc
    45181 ccaagcagaa ccgccgaaaa tcgatgtcca attattcggt tctttgccat ctaccccgtc
    45241 gacccaaatg taataatcgc gatacgggct ttctttagat tttttcgatt cttcaaacca
    45301 ttgatgctga tccgaactat ggttgatgac aagatccata atcactttga tccctaattc
    45361 atgtgcttct tttagcaata aatcaaaatc ttccatcgtt ccgaatctct cttctatatc
    45421 ttgatagtca cttacgtcat aaccattatc gacaaatgga gattgataga tcggattgat
    45481 ccagataaag ccgataccaa gatctttcaa ataattaagt ttttcacgga tcccattgat
    45541 gtctccttga ccatcgccat tgctatcttt gaaactagca ggatagattt gatagcctac
    45601 tgcttctttc caccattttt gttcttgtat catatcgttt cactccttta acggtccctc
    45661 ttgattataa ccgattctat tttagaagta tccttgttac cggtaactag aaacgaaaag
    45721 ctcaacgaat ctgctaagtg cgttcgttga gctttatttt atcatagata gcgggcaatc
    45781 agttgaatca tttgcttggg aaattcattc aagtcagtaa tatctacaaa acgactttca
    45841 ccgtagatcg acttgatttc ttctttgtct tggccaatag ctgcggagac aaataggatt
    45901 ccttgacgtt catattcttt caaaacagac tggatatctt cttttgcttt tttgcctgta
    45961 taatccggca acgcttttgg ctgaccgtca ctaatattaa gcaacagttt ggttgttgcg
    46021 gattgtctgt ttaatttttc tgctgctaaa cgaagtgcag caccatcacg attgttttgc
    46081 cttggtttca tagtgactaa tttttcattt aaccattgaa atccgtcttc aaattcttta
    46141 taagaaaaca aagaagtctt ttctcttgac gaacggtctg ctgtatctcc gtaaatcaac
    46201 aatgggatat cgactttctt agaaaattcg gcaatggcaa tcgctgcttt ttttgccgct
    46261 tcgatccgat catcccgtat cattgaccca gattcatcga tccgtacagc taccgctaaa
    46321 gaaggttgct catgtggcgg gttctttttg tcaaaatttc tcaaatcacc atatgctact
    46381 ttgcttgcat tgaaccgctg accttcgtat tttccttttt ggtacgtctc tgtttgttca
    46441 ttatccagta actctaaaat ttgccggcta agtgattcta ctattggcat tacttgcatg
    46501 tgtagctcac gtgcttccgg cgtttttcct tggaaagctg gtcggtgaac gatgagacct
    46561 tctttttcat gaatcgtttc cttcatgatt tgtcgcgctt ctttattcag ttgcttcttg
    46621 atttccttat catgtgcttc catgtcagaa gcagacattt cttgagaatc atctcctgtt
    46681 tgttgttctt tttgattttt tccttcgata tcgctactgc gttctttttg catttgagga
    46741 ggaatctcgt ttttttctat tgtttcttct tgtttctctt ctccctcatt tggttcttca
    46801 gcagacaacg atttcttttc atctaagcgc agaatttctt cagcattttc agtgatgttt
    46861 cgttcatttg cttcttcggc gctaatggtt ctttcttttg tatgctcaga ttgaggatga
    46921 ccccattttt cttttctcaa tatgtcctga agttccagta gcaatccact gtcagacaaa
    46981 gcttgttcaa gaattgccaa ttcatctgaa tcagtggata atttatacaa aacttttgga
    47041 taaatcgact ggagtaaatc ttgtccttgc ttataggtat ttgcccaata aaaataagaa
    47101 cgcattcctg ctacaccttt gatggcgtta gcttttgcag tttcatccaa caaacggata
    47161 atctctgcca tctttaagag aagttcttgt ttttggatca tagttttcgc catcgcacgt
    47221 tctgccatga cttctaaggc aggaagatcc attttttccg catgttgcat ccgatctctt
    47281 aaagattcat ttaatgggcg atttccttgg taatttcgat ttgtagtgat cacaatgacg
    47341 caatctggat ggcgacgaat gattcctgtc ggtaaattca acatcccatt tggttcgagc
    47401 gctgagttaa gtgccacaag aacagaggca tctcggatga cagtcggctc ttgaatctct
    47461 aataaatagc ctttttcaat cgctcgaacg atttcagaag ggtaatagct gtaatggatt
    47521 gcatctggat aatcagcttc gatcctttga actagctctg ctaatttttc tttagcagaa
    47581 aaaggatcaa tgccgtaatg gttctggatc agtgtcagta cagcttcaag tgtatctgtt
    47641 tggtaaatag cttctagtaa ttcttggtct tgttccttat tagaatctac tacaggtaat
    47701 aaggctccga aaacatctga tttgtccata tctgcaaaac aggtcacttt tgtataggga
    47761 agttgcagat cagcagatag tgctttggct aattgtgtct tccccgaccc ggcatctcct
    47821 tccaaaagta cgttactgat tttcatttca ggatcttgcc aattcccttt gatttcggag
    47881 gcgatccgcc attcttctgc acttgtccga tggcttttcg gtttcttcca gatcatttgc
    47941 ttttctagcc gtgaaaaggt tcggttttct gttagtgaga aattctccat gcttctcttc
    48001 cccttttgat catctctgtc tattaatatc ctagctcatc taaaagacgt tttaaaatac
    48061 ggaatcgttc cccaatcagt tctccgtctt tttgtagtgt atcgtaatac aaaatgatgt
    48121 cttcatcgat ctttggattt ttggtatcta cttctaccgt cattcggttc ccttgtaatc
    48181 caatgatgtc tacgactgga ttttctggat cataaaattt ttcataacga taagcatctt
    48241 ggaagtataa acttccttgc gcagctaaga tcgctaaatc taaaatacgt gtgattggta
    48301 attcttccga ctgtcgtgac catttttctc cagtatgtcg ccataccttg cctgagatat
    48361 cgacttttcc acgatcattc cactgagcaa ggcccagtga aagccctttt gcatccatat
    48421 ttaatgcatt tcttccatca actttatcat agtcttctac aacgattact ggtttatgtt
    48481 ttaaatttgt tggtatttcc attattttaa ctcctcatcc ttttattttt agtaaataag
    48541 taaattagta gtttactcat ttactaaact atatgcgaca ttatagagga ttgaagtgag
    48601 gattgcaaag aaaaagagct tcattgatag aaataaaaac accatctcgt tcttttacac
    48661 gctcattagt aacatgtaga aagtccaaga tgatgttgga atattttggc tctatacttt
    48721 ttgatgttgg tgaacttcac caacatcttt tttgta
//
LOCUS       DASHHB010000015        48553 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.015, whole genome shotgun sequence.
ACCESSION   DASHHB010000015 DASHHB010000000
VERSION     DASHHB010000015.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48553)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48553)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48553
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.015"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(13..378)
                     /locus_tag="U2K71_000950"
     CDS             complement(13..378)
                     /locus_tag="U2K71_000950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315055.1"
                     /translation="MKKVTREEVASILVKDKYFSKGNYWLKIWQTLVALLGWMIVVLP
                     FIWVFFPLTRPERAKDFSLYAFLSEKKMLYFLIGFFVLIFFSFLITSFVLTRWNNRNL
                     YKKVNKSFEYAVCIIKRDK"
     gene            complement(553..2688)
                     /locus_tag="U2K71_000951"
     CDS             complement(553..2688)
                     /locus_tag="U2K71_000951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM7315056.1"
                     /translation="MVNTQKQLEEAYLKNVYQKLIDKKNELRESMDTAKIEGNQSLKE
                     MAGELRLNFDSYLDNLDTFSMIEMKNREIDQMNIKQKNAENQLAKIERLLKSPYFGKV
                     VVDFLDNEPKESFYIGTANFTDDADENLVYDWRSPIAELFYNNMIGPSSYTVRQNKID
                     TVIKERRQFLLERDRLVKFFDTSVAIQDDVLLEVLGQDETNEMKAITATIQSEQNTII
                     RDVKSQNILVNGVAGSGKTSAIMQRIAYLLYLYRDTMTSDDLLILSPNHRFIDYISNV
                     LPSLGERNPLNLTIIQLVSQLSAEEIEGEEAYFKRITGENVSEQTERLRSKKFIDNLK
                     QSDPLFLDHPNFIRGLTKNGKTVLSKKTIEKIYEKVPAHPKLIDRLQATKKALMSEWK
                     NHLLKQAKSPAVQNQVLSLTEDRQLELFGKLISDDSEQSIVAYARKLLQKKYRKITRQ
                     IEEMAWVDEHQLFERIYEKRYGSAYAWQPTRTVDEAVIILAIRHLLVEKVNVPAFRYL
                     LIDEVQDYTLAQLGLLIELFPKTHFTLVGDENQAIFNSSTTFADIMRCFDDYHLPIHR
                     YDLRNSYRSSGAITELFKTYAVDQEKIDIVSIRPQGKEPEYRSFRSSEELLKILAEIL
                     VSLKGEKAAVITQTEEETQTLQESIKKNSYGLENCQIIPLSLAKGLEFDHVILYPFEN
                     DGDEQRRRRQMYTAISRGMKSIVVLERAT"
     gene            complement(2771..4426)
                     /locus_tag="U2K71_000952"
     CDS             complement(2771..4426)
                     /locus_tag="U2K71_000952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM7315057.1"
                     /translation="MKIVIVGGVAGGMSAATRLRRLMEDAEIVVFEKGPYVSFANCGL
                     PYYLSGEISERENLLVQTPESLSARFCLDVRPNHEVTAIFPENKTVEVVHEGQKHIEQ
                     YDALVLSPGAKPVVPSIPGITEADNVFSIRNVPDIDKVMHALEKQPKRAVIVGAGFIG
                     LEMAENLKRRGLEVMVIEQAPHILPTLDEEMAAFIEKELSHQGVEVITSHAVAGFEDH
                     GKRLRLDDGRTIPADLVILSIGVRPDNQLAVTAGIELGIRGGILVDERYQTNIPDIYA
                     VGDAIVVKQQITGKDALISLASPANRQGRQVADTISGISRRNQGGIGTAIIRTFGMTA
                     ASTGLSERTAKENELSFEVIHVSGKDHASYYPEATDILLKLIFHPETGEIYGAQGVGA
                     KGVDKRIDILATAIKGHLTIFDLPELELTYAPPFGSAKDPVNMLGYAAMNIAEGLSET
                     VQWHELPTELAKGKILLDVRTAEELEKGKFKEAKHIPLNELRDRLNELDSQQEYIVSC
                     HSGLRSYLAERILKQSGYHVKNLDGAFSLYQTVRPEELIYPNK"
     gene            complement(4428..4742)
                     /locus_tag="U2K71_000953"
     CDS             complement(4428..4742)
                     /locus_tag="U2K71_000953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEM7315058.1"
                     /translation="MNLLFQSIKSITVPELKEKILENPALLDVRTPAEYRGGHIKGAK
                     NVPLQSINRYDGDKEKTVYVICQSGMRSKQAAKELKKSGYDVVNVRGGMNQWFDRTVG
                     GK"
     gene            4912..5172
                     /locus_tag="U2K71_000954"
     CDS             4912..5172
                     /locus_tag="U2K71_000954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sensitive transcriptional regulator"
                     /protein_id="HEM7315059.1"
                     /translation="MEIDNKNILNRLKRTEGQIRGIQKMIEDEKECMDVITQLSAVRS
                     SIDRVMGMIVADNLKNCFEKPDSNPEEQAAKLEQAIKMIIKK"
     gene            5186..5500
                     /locus_tag="U2K71_000955"
     CDS             5186..5500
                     /locus_tag="U2K71_000955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HEM7315060.1"
                     /translation="MKVVFHIDELEKWSETGKNVKNLIKASPETTIVVSVNGIAITGY
                     LDSANAEFLDLQGVVFHACANAMRANHISESSLPEQVIVVPAGVLDLVELQSQGYAYI
                     KP"
     gene            complement(5985..6539)
                     /locus_tag="U2K71_000956"
     CDS             complement(5985..6539)
                     /locus_tag="U2K71_000956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HEM7315061.1"
                     /translation="MKLSTDWRKEIQTIPNLLSIFRIFLLPIYLYFVLRQSFYIAGAV
                     IVVSGLSDYLDGVIARRYNQVTDLGKVLDPFADKLTQLFLILSMAWYRPWLWLLFGLF
                     LIKEGFMFVAGLIGLSKNIKLSGAKWYGKVATAVIYVGMILLLLFPELPTLWVRVIFA
                     VITYGLLQSFVLYAVEYRKMFQRK"
     gene            complement(6665..8593)
                     /locus_tag="U2K71_000957"
     CDS             complement(6665..8593)
                     /locus_tag="U2K71_000957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease MutS2"
                     /protein_id="HEM7315062.1"
                     /translation="MDNEMISQLQFDRIKKEIQARAIGNYSKKRISDMTVSTNLQTVL
                     DRQEETREARLILESSQHVPFMGLPRIDALTEQVKKGLILQPTDLIEYADFLRSSRMI
                     TKFFDKNQYQTPLLFAYSKHLPDLINVEELIDQKIKNNKVSDDASRNLRKVRKQLQLI
                     EKEIQSKLLKFLRHPKNKEMIQEAMIVQKGEYYTIPIKASYKNKIDGTIIDESNKRTT
                     VFIEPTVISKLNEHYQLLKAEEISEEYQVLAALTGAIAENEEVIDLLIETITVLDIIF
                     ARAKFSREINGSTPRINKSEHIIIKQGRHPFLPEHAVPLDVEIGKDYRGLIITGANAG
                     GKTVVLKTVGLLTLMAMFGMQVPAKEGTELAVFDEIFVDVGDHQNLENALSTFSGHMQ
                     NIAAILKKIKRNTLVLLDEIGSGTEPNEGAALAIAIMESMYEQGALIIATTHYGEIKK
                     FAGDHEDFVPAAMAFDREALRPKYQLRVGETGESQALWIAHKMDMSMKLIHQAERYLA
                     EKAYRTQKKEFLPKTAVTNRKENKKERQLFAKGDRIFVNEYQKEALVYEDIGEDTIDV
                     YLDKKIIHVPRQRVRLVRSAEDLYPTGYDLDSLFIDYKTRKRQRDLERGSKKAHKVLV
                     KEMRKRQEERRVNDENSK"
     gene            complement(8899..9615)
                     /locus_tag="U2K71_000958"
     CDS             complement(8899..9615)
                     /locus_tag="U2K71_000958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEM7315063.1"
                     /translation="MNSDMTQIMGEFIGTLILVLLGDGVCAAVNLNKSKAQASGWIVI
                     AFGWGLAVTMAVYISGFMGPAHLNPAVSLAMAMTGAISWNLVVPFIIAQVLGAFAGAI
                     LVWLSYLPHWNATKDESAILGTFATGPAIRNYPANVITELIGTFVLVLGLLAFGQNEF
                     APGTNVFAVGGLILAIGLSLGGPTGYAINPARDFGPRLAHAVLPIANKGTSDWAYSWV
                     PIAGPMIGAIIAVGVYSLMV"
     gene            complement(9616..11439)
                     /gene="glpO"
                     /locus_tag="U2K71_000959"
     CDS             complement(9616..11439)
                     /gene="glpO"
                     /locus_tag="U2K71_000959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glycerol-3-phosphate oxidase"
                     /protein_id="HEM7315064.1"
                     /translation="MFSNKTRQDSIQKMQQEELDLLIIGGGITGAGVAVQSAASGIKT
                     GLIEMQDFAEGTSSRSTKLVHGGIRYLKTFDVEVVADTVGERAVVQSIAPHIPKPDPM
                     LLPIYEGEGATTFNMFSVKVAMDLYDKLANVTGTKCENYTLTPEEVLEREPFLKKEGL
                     KGAGVYLDFRNNDARLVIDNIKKAAEDGAYLVSKMKAVGFLYDGDQIVGVKARDLLTD
                     EVIEIKAKLVINTSGPWVDKVRNLNFTRPVSPKMRPTKGIHLVVDAKKLPVPQPTYFD
                     TGKQDGRMVFAIPRENKTYFGTTDTDYQGDFTDPKVTQEDVDYLLDVINHRYPEANIT
                     LNDIEASWAGLRPLLIGNSGSDYNGGDNGSISDKSFNKVVDTVSEYKENKVSRAEVED
                     VLNHLENSRDEKAPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKE
                     EYGIETKEIDSKKYQISGGNFDPTKLEETVTELAKEGVAAGLEEEDATYIADFYGTNA
                     RRIFELAKEMAPYPGLSLAESARLRYGLEEEMVLAPGDYLIRRTNHLLFERDQLDEIK
                     QPVIDAIAGYFEWTEEEKAQQTKRLEALIAESDLRELKGEK"
     gene            complement(11453..12949)
                     /gene="glpK"
                     /locus_tag="U2K71_000960"
     CDS             complement(11453..12949)
                     /gene="glpK"
                     /locus_tag="U2K71_000960"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HEM7315065.1"
                     /translation="MTESTYIMAIDQGTTSSRAIIFDKKGRHIGSSQKEFTQYFPQES
                     WVEHDANEIWNSVQSVIAGAFIESGIKPNQIAGIGITNQRETTVIWEKDTGRPIYHAV
                     VWQSRQSAGIADNLKNEGYQEFFHEKTGLVIDAYFSATKIRWILDHVEGAQERAEKGE
                     LMFGTIDSWLVWKLTDGQAHVTDYSNASRTMLFNIHQLDWDQEILDLLNIPRVMLPKV
                     TSNSEVYGYTQGYHFYGSEAPISGMAGDQQAALFGQMAFEPGMVKNTYGTGSFIVMNT
                     GEKPQLSKNNLLTTIGYGINGKVYYALEGSIFVAGSSIQWLRDGLQMLQKASDSEDAA
                     KRSTSEDEVYVVPAFVGLGAPYWDQAARGAMFGLTRGTTKEDIIKATLQSIAYQVRDI
                     IDTMQDDTGIKIPVLKVDGGAANNEYLMQFQTDILNVPIQRAENLETTALGAAFLAGL
                     AVGYWKDTDEIREFYEAGKLFEVQMEEERREKLYNGWKKAVKATQAFE"
     gene            complement(13085..14545)
                     /locus_tag="U2K71_000961"
     CDS             complement(13085..14545)
                     /locus_tag="U2K71_000961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315066.1"
                     /translation="MKIEALLERKEQLQILILRNLVLQGGTASINGLREHVQLSKASF
                     DQYLEDIELIGRMMEKKVEVQRNEYQALLVLDEDVSLEKILLFLLQESLKFKMLVYLL
                     EHQQVSIVRLATAFNISESSVFRKIKELNQLLEEFGLQIKNGQLYGEELQIRYFYYEL
                     FQYIPEDQRPLFLQNTPEKRPFILGLDRVLETTFTASAEAQIACWLGITKKRLLNEKS
                     TYATLKEKKLLYQSDRLYQAIDPIIVMHLSRTAAELNVYETMMFYSFFVSFSIVDEEI
                     FYQYDLTRSKKLPTAVLDTYIRETMLWHYRPRRLKIKEEKAVGYQISQINNEWFFFVG
                     KIEVYERDRLLEQQQKMLGHSLTQLLAKLQETAVQQLPRKRAEDSELSYLMIQYANVL
                     LMIDFYIAKPVVIGIDLESLPIYRIAFQQYLIRELRGIGGIEIGSYEEGKEYDLVITF
                     CQRNKKQCEYYLSEFASPYDIIRLKRRIETLKKEKN"
     gene            complement(14535..15830)
                     /locus_tag="U2K71_000962"
     CDS             complement(14535..15830)
                     /locus_tag="U2K71_000962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM7315067.1"
                     /translation="MNIVIVGGSFAGIHAAIYLRQLMPTSEIYLIEKQSKLGWIPSGF
                     NLILKGKVTSEKQLSWITKEELTDRYRIHVYTEKTVIGLKEKKVVLAGGKKLDFDRLI
                     LATGSNQKFRNISAESTLIHPVKKIQNVTALQQKIMNAKKIAIIGAGQAGIEIAEGLA
                     SRKKQIHLYESRKSILFRYLDPEMTEPLVKEMKNQGLSLFLEEQVVSLTENETAIVET
                     EKRKENYDLVLLANHSRPDNQMWEEQLTLNDDGTIWVDDYLQTSQKDVYAIGDAIQVT
                     FRPTNEKMYVSLVNNAVRTARNVSKTISGSQTKDQGTYRPVGNQWFGYFLGSVGLTEE
                     ESIFYPQKIETGYVDYKLTAINQSKVKLKFLFDSEKKILGAQLQSKKEMFHLLDQLTI
                     AVKEGWTFEQLEEHELFFQPEYRVPDMVWSKVANTAYED"
     gene            complement(16162..16839)
                     /locus_tag="U2K71_000963"
     CDS             complement(16162..16839)
                     /locus_tag="U2K71_000963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HEM7315068.1"
                     /translation="MKVLIVLTNVEKYEKIERPTGLWLSELTHFYDVLVKNGIDADFV
                     SPKGGYVPLEPQSVLNMDEIDWAYYEDEDFRNRALGNTLRPDDVDANDYAAVYYAGGH
                     GTMWDFPEAIDLGKVASQIYRNEGLVSAVCHGVVGLLPVTDESGKSILAGRTVTGFSN
                     EEEEANGTTNEVPFLAEDALKEKGANYQSGAAFSEVVQIDGRLLTGQNPQSAHQLGEA
                     VVEALKN"
     gene            complement(16869..17960)
                     /locus_tag="U2K71_000964"
     CDS             complement(16869..17960)
                     /locus_tag="U2K71_000964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-dependent flavin oxidoreductase"
                     /protein_id="HEM7315069.1"
                     /translation="MSQFDQSITLPSGVQLKNRLMLSPMTTQLSFFNGVITEDEITYY
                     KQRSKGLGAVITGAANVQDIGKGWPGELSIAHDEMIPRLSELAKAIQGEGAKAIIQIF
                     HGGRMTSRATLSGEQPVSASAVAAKRPDAETPRAMSEEEIVETIQAFGKATRRAIESG
                     FDGIELHGANTYLIQQFFSPHSNRREDQWGGSLENRYRFIEELLTTVFSAVEQYAKKP
                     FIVGYRFSPEEYETPGIRFEDTIWLLERLRESKLDYLHVSLNMYDRIARSDKYNDKSI
                     LEVVHNTIQGKIPLVGVGGIRNREDVEQVLKYAELAAIGQQMIVDPDWAEKLLENRED
                     EFVTKPFEEAYKELYLPSPLYNFLKARYQ"
     gene            complement(18061..18495)
                     /locus_tag="U2K71_000965"
     CDS             complement(18061..18495)
                     /locus_tag="U2K71_000965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM7315070.1"
                     /translation="MEKPIEKALDECLFFSVKKLDRMLNKLADEAFRRTGLAPTYGFI
                     LLILKEKDGIPQKDIAQMLYSAPSTIARFVEKLEYKGYVKTVSEGRLSLVYLTEQGRQ
                     FAKEVDGSWEELHQSYKAVLGNEASKELAQTMNDATDKLQKK"
     gene            complement(19079..20536)
                     /locus_tag="U2K71_000966"
     CDS             complement(19079..20536)
                     /locus_tag="U2K71_000966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001336277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM7315071.1"
                     /translation="MKKFPEGFLWGGATAANQCEGAWDVDEKGLSVSDVYTFDSDLPK
                     EKWTDQWHMMTHQQVKAAQDPNSKYYYPKRHGNDFYHHFKEDIRLFAEMGFKCYRMSI
                     AWTRIFPHGDEETPNEAGLFFYDQVFDECLKYGIEPVVSLSHYEMPLYLVTEYGGWPN
                     RKLIQFYVRFAETVFKRYKNKVKYWMTFNEINCVKHHPFVSVGVIEENHPHLEQAKYQ
                     GAHHQFIASALATKACHEIIKGSQVGCMISYQLLVPYSCDPDDIQKTIEQQRTSLFFS
                     DVQARGYYPAYTQRMFEEKGVNLKIEVGDEEILAAYPVDFVSFSYYMSSAVSAHPENL
                     EGVQGNLITGGIKNPYLPSSEWGWQIDPKGLRIALNQLYDRYQKPLFIAENGLGAIDK
                     VEEDGTVQDDYRIDYLKQHIEQMKEAIEDGVDLFGYTSWGCIDIVSASTSQMSKRYGY
                     IYVDQDDEGNGTKKRLKKKSFDWYRQVIQSNGEIL"
     gene            complement(20533..22428)
                     /locus_tag="U2K71_000967"
     CDS             complement(20533..22428)
                     /locus_tag="U2K71_000967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM7315072.1"
                     /translation="MEKPFSKNYTYLVRRIATALINKGEVKMDYSQLAKDIVRFVGGE
                     ENVSNVYHCATRLRFTLKDNKKADKEKVEQLEGVITVVEAGGMFQVVVGNAVNEVYDV
                     LSKQMKLEDDASSGKRGTEKKGILNSFIDMIAAVFAPTLGVLAGSGLIKGVLALCTSL
                     NLLTTESGTYIILNAAADAFFYFLPIFLAYTAAKKFNTDRFIAMVIAAALVYPTIVSA
                     YSDSITLRFLGMPVILARYTSTVIPAILAVWVLSYIEPKIRKSLHESIRNLLTPFICI
                     IVMVPITLLVVGPIADYASQLIAAGYLAVYNFSPVLSGAVIGGFWQVLVIFGLHWGLV
                     PVMTNNLSFYGRDTLGPACMTAVAAQAGAVLGVFLKTKNKKVKSLSLSAFITALFGIT
                     EPAVYGVTLKYKRPFYIACICGAIFGGVAGAAGAGALAVATRSILSFPIYIGEGFVWL
                     VASYFLAMISSCMLTFLFGYKDEIEEESSKDIVLSTPAAGEIIDLSEVNDPTFASGSL
                     GEGFAIIPTDGKIYSPVNGEVSTVFPTKHAIGVVSEEGAEILIHIGIDTVNLNGKYFQ
                     SAVSDGKKVRKGDLLMEVDLQELIKEGYDPTTMVIVTNSSRFKNIITSHKKNNQTRKL
                     ASLGGSI"
     gene            complement(22486..24261)
                     /locus_tag="U2K71_000968"
     CDS             complement(22486..24261)
                     /locus_tag="U2K71_000968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEM7315073.1"
                     /translation="MFITKRRCKQMDLNKRQLQILVVLRNKNHWITSEELAEALGTNK
                     KTIQTEIKNMLAIYEKKIIIQSNKRSGYFLESIESETKQQIIQEVTKHKIYSSMDFRA
                     STIVTYLLFQKGYVSMQKIADIFFLSKTAVSLQIKTILRWIERNPKLELEVSSTKGLK
                     IIAEENSKRIFLSLVGTETVIQHARLPQQISTIFSNIMPTVQKTLKEVLISYNYIVSG
                     EDFIRFSRYLVLTILRREELLEKTNRYTLFIPMVETLTKKLSSELPYSFSNQEKQAIE
                     NRLLELNYLMINDEKNKEIEKNLRAFEQTIIRFLDLPVSLLFHETEFLLTHIKQMKTR
                     MDAGHNTMNHFVQKIISRYPLEIYLIRRFFPIHFQLRPNLAESAYLVLYLAEALNPFR
                     KQGEILIVSNQPISILNTLKKQIQQSMNMWIKECRIEPVYLFKNQSNKITEYDAILTT
                     EQELIFEWDDLIYLPSVLDEFESSEIYQILREKLTIVEEKKKTKLLDHYYKEENQIKI
                     HKKIDSIQQLISPSSDLVMLPLNAETLLCCLIKSQVETKITEYQLEIPLIHQQRKIKR
                     VFFVSFDYKRNDILVFFQAVSDLLG"
     gene            complement(24311..25807)
                     /locus_tag="U2K71_000969"
     CDS             complement(24311..25807)
                     /locus_tag="U2K71_000969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM7315074.1"
                     /translation="MDQEIFPENFLWGGAVAANQCEGAWLEDGKLPNVTDTLIGIMNQ
                     HPSIQWNEEKKIWEIALDESLHYLSHEAIDFYHRFEEDIRLLKELGLKAFRTSISWAR
                     IFPRGDEQKPNEAGLVFYDRLINTLNRYDIEPVITLSHYETPLALVGEYGGWQNRKLI
                     DFFEFYAQTVFERYQGKVKYWMTFNEINNAFRMPYAAAGLVTFPARDKLEPIATLTKK
                     EIYQACHHMFLANARATQLLKKIDPNAKMGIMCSFSALATYAYDCDPENVFGSLQFKR
                     NSWFFSDVMCRGHYPAYIYRIWKEEDCAPVMLDGDEQYLQANTVDYIAFSYYRSAVYK
                     KEAEMRVDTGGANGFDNPFLKEKSPEPWSWPIDPLGFRYVMNELTDRYELPLFIVENG
                     IGLDEAPDELNRIHDPKRCEYLKIHLQEIAEAIKDGCQIIGYLWWGPIDIVSAGTGEM
                     RKRYGFIYVDRQNDGSGSFRRLKKDSFEYYKQIIQSNGKNLEYRSFFK"
     gene            complement(26001..27623)
                     /locus_tag="U2K71_000970"
     CDS             complement(26001..27623)
                     /locus_tag="U2K71_000970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000415199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glucosidase"
                     /protein_id="HEM7315075.1"
                     /translation="MEQKWWHHSVVYQIYPRSFQDSNQDGIGDLRGIISRLDYLAYLG
                     IDAIWLSPVYESPNDDNGYDISNYQAIMAEFGTMEEMEELIEEAKKRNIRIIMDLVVN
                     HTSDEHPWFIEARKNKENPYRDYYIWRDPVNGKEPNRLRSIFSGSAWQLDEKTGQYFL
                     HLYSKRQPDLNWKNKQVRQEIYQMMNFWIDKGIGGFRMDVIDMIGKEPDKEITSNGPK
                     LHEYLQEMNQATFGDKNLMTVGETWGATTEIAKLYSNPKRNELSMVFQFEHIGLDQQE
                     GKDKWDLKPLSISELKKVLSKWQTSLGDEGWNSLFWNNHDLPRIVSRWGNDDVYRKES
                     AKMFAILLHMMKGTPYIYQGEELGMTNCPIDDIAEAKDIETINMYNERISSGFTKEEI
                     LESINAKGRDNARTPMQWNAREHAGFTTGIPWLRVNPNYKEINAEEALADQDSVFYMY
                     KKLIDLRRKHDIIVWGDYELVEKTPEHVFAYSRIYEDQQWLVICNMSSEKQTFTVPRE
                     ALRVIVSNYPLKQLPDGKVDLRPYEAFVLEVK"
     gene            complement(28073..29386)
                     /locus_tag="U2K71_000971"
     CDS             complement(28073..29386)
                     /locus_tag="U2K71_000971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEM7315076.1"
                     /translation="MKEKILSYFEIEKLNTTVKREVLAGFTTFISMAYILFVNPTVLG
                     ASGMDEGAVFTATALASAIGCILMGVLARYPIGTAPALGINAFFAYSVCLGMGIPWET
                     ALAGVFVASLIFILITIFKLREMIIDAIPADLKYAISGGIGLFIAFLGLSEGGIIVSN
                     ESTLVGLGSLNVGSTWLTIFGLVVTAILLVRRVPGGIFIGMAATTILGLVTGLIPMPS
                     EIVAAAPSLKPTFLVALKHVGDINSLQMWVVVLTFLLVTFFDTAGTLVGLANQAGFMK
                     DNKMPRVGKALASDSTAMLAGSLLGTSPVGAFVESSAGIAVGGRSGITAITTGIFFLL
                     GLFFSPLLSVVTSQVTAPALIMVGVLMAQSLRQIEWGNLEIAIPSFLILIGMPLTYSI
                     SDGIALGFIFYPITMLAAKRGKEVSPIMYGLFFVFMGFMWILNVK"
     gene            complement(29631..29855)
                     /locus_tag="U2K71_000972"
     CDS             complement(29631..29855)
                     /locus_tag="U2K71_000972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315077.1"
                     /translation="MDLMGRLEGYLKYRRAKHSEKLRAKNLKSGKKKTKGWGKMVDTG
                     FGGLNNLNPVDTKEFIKEEQKRNGKIKSHY"
     gene            complement(29875..30297)
                     /locus_tag="U2K71_000973"
     CDS             complement(29875..30297)
                     /locus_tag="U2K71_000973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEM7315078.1"
                     /translation="MESVYRQERKSNRKKGQTMIQKTRVMLYVNDVEMICRFFVEKIG
                     AEISETIELPEEFKSIVLSISKELELGVFPKVFVQKFSPEVLGPPPSLVFFTDEFETI
                     YENMEEPGEITDNNGVLTFNFSDPEGNYFVIGKAESSD"
     gene            complement(30439..31149)
                     /locus_tag="U2K71_000974"
     CDS             complement(30439..31149)
                     /locus_tag="U2K71_000974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315079.1"
                     /translation="MKKVIVDTNFWEVFPEATIEILSVNGIDNHVTKESEETYHQLLN
                     EAAKEARNYLTEETFSQNEVIAQWRQAFTTFKTKKGARSSIEALLKRANQEREFFPIN
                     PLVDIYNSISLSFGVPCGGEDIEKIAGNLHLGKAQGDESFLPLGADSDAPALPEEIIY
                     YDEQGAICRCLNWREAQRTMLTEETQAAVLVIESINAEQAARGAQAIEALKEKIDQTF
                     GVTSVRHTLTKDESEAEL"
     gene            complement(31167..31663)
                     /locus_tag="U2K71_000975"
                     /pseudo
     CDS             complement(31167..31663)
                     /locus_tag="U2K71_000975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321138.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            complement(31700..32152)
                     /locus_tag="U2K71_000976"
     CDS             complement(31700..32152)
                     /locus_tag="U2K71_000976"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM7315080.1"
                     /translation="MAEILRDIGMIARALDSISNIEFKEVDLTRGQYLYLVRICENPG
                     IIQEKLAEMIKVDRTTTARAIKKLESNGMIERLEDKENKKIKKLYPTKKGAEIYPFII
                     RENNYSNAVALNGLSDEEAKQLEYLLNKVCKNVSEDWNFVKKGNKRIY"
     gene            complement(32287..32892)
                     /locus_tag="U2K71_000977"
     CDS             complement(32287..32892)
                     /locus_tag="U2K71_000977"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM7315081.1"
                     /translation="MNKKLAQYKRLLALSQAGLYYGKDVFDKERFEEIRTISLELISE
                     IGKETFEEIKALTTIGEGHPTPKVDVRAYIKKDGKVLLIEDKRTKEWSLPGGFAEIGL
                     SPEENVRKEVYEETGLTVETTQLRAVFDTNKQKDIPQLFQYYKLVFACAIHGEEAKFI
                     ENNETSNMGFFSIEELPKLSEKRTTKKQLLILENKNQIYCD"
     gene            complement(33342..33962)
                     /locus_tag="U2K71_000978"
     CDS             complement(33342..33962)
                     /locus_tag="U2K71_000978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM7315082.1"
                     /translation="MKKYFILLAGSPGTGKTYLMNKLRERFPDMYAITLDEIKEYYAE
                     SIGFNNLEERAEQERTKVYPFYYKALDLYMEAGKKVIVSEYPFSDKQKARLQELADRY
                     GYQVITIRLTADFEVLWQRRYKRDRESDRHLSYIMDHYHYGDVLEDRTKGTNHITKEA
                     FKKIIADRKYDKFQLGTLYEFDVTDYTQVDYTPLLDKLEKEINDNK"
     gene            complement(33964..34833)
                     /locus_tag="U2K71_000979"
     CDS             complement(33964..34833)
                     /locus_tag="U2K71_000979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase family
                     protein"
                     /protein_id="HEM7315083.1"
                     /translation="MYTTLKEVTKTAEELNFTVGAFNTHNLEMLPAMLGAAKEMGSPI
                     IIQTSVDTAKYIGMHVLVNVMTAIAEEEMVDAVLHLDHARDFDDIKEAIDSGYTSVMY
                     DGSHLPFKENILKTKAVVEYAHAHGVSVEGELGTIGGTEEGIHVAENDKVYTRPEDAV
                     KFVEATGVDALAIAIGTNHGQFKSKTEVNIPLLKEIDAVVDVPLVIHGGTGVKEEDYP
                     ELINNGIRKFNVGTELLVNWTKVAKETFSETEINKSLRHNVIPANEAVKEIVKHKIGL
                     FMNLESPINLGNK"
     gene            complement(34856..35263)
                     /locus_tag="U2K71_000980"
     CDS             complement(34856..35263)
                     /locus_tag="U2K71_000980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HEM7315084.1"
                     /translation="MFGFFKKKEKEPKKVLAKEEKALSPEATEKIQSEITQLKQEIST
                     TQDKHKLAKLYEQVGLKFSELYVNDQAIQYLEKSLENKQTIGDGYKKLMSLYNQKRAD
                     AARAGDDQGIDYYMGKMDEMRQIAKQVTIKGNK"
     gene            complement(35282..36571)
                     /locus_tag="U2K71_000981"
     CDS             complement(35282..36571)
                     /locus_tag="U2K71_000981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7315085.1"
                     /translation="MGVINFIIENILTQASITIALIAMLGLLLQKKSAGQVISGTLKT
                     LLGFQVLSAGSSIIVGSLTYFGKIFTEGFHMQGIIPSIESINGQAMNDLGLGRDIALT
                     FLAIFVFNIILARFTKWKYIFLTGQAILWMATMTTVFGYFAGLRGIVLILVGGFIGAC
                     FAIAMPAVAQPIIRKITGSNDIALGHFCTIGYLFEAGVAKLFGEKGENKKSIEDIKLP
                     THFEFLQDTYLSVMVVMVPLYIITVLFAGEPFASELSGDQNYIMFAFLQAIQFVVGVY
                     VLLAGVRLLLGEIVPAFRGIAMKLVPDAIPALDCPVFFPYSPNAVILGFITTTIGTII
                     AMFTLPMFGLAMILPGMLTNFFAGGTAGIFGNAVGGRRGAIIGGIAHGFFITLLPALL
                     VTIFNSMGFINATATDVDTVAAALLYAWILSPVLKAF"
     gene            complement(36598..36900)
                     /locus_tag="U2K71_000982"
     CDS             complement(36598..36900)
                     /locus_tag="U2K71_000982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7315086.1"
                     /translation="MNKLNILFVCGAGLGSSFAAQMSAEDVLNKLGVEAKLDHCDISS
                     AVSMKPDVIITAENFRSQFEKFAVDPKTAMIYLKNIVSKNEIEEKITPVLQDKGVI"
     gene            complement(36925..39018)
                     /locus_tag="U2K71_000983"
     CDS             complement(36925..39018)
                     /locus_tag="U2K71_000983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM7315087.1"
                     /translation="MKTRTIEILHRIMTAQYPVKAKHLASEFNVSQRTIRQEVLEANI
                     WLSSKKLPEIRTIRNKGFLLKLEKEKQARLETEIKDVKDGFLNRRERIFDLVLAVAYS
                     RIPIHLNKKEEEFQISKSTLDEDMRRLRATLVSYGIEIVSYGKQGLVYKGSERSIRTM
                     IYDFINQNLGMTDFFMNQDAQLTPAQKIFLHYFPLEEIKKIQEIYSNKLLKQEDDIYK
                     KQILLFTLIWIQRIRKHEFISAINWKNTEIKDSSFSAFIDHMIEEFQLDNVPQVERNY
                     LRFTIETFNARDISNSIEWVQAQLLTIQLIQFVENETHVPFHLKEEALFENLYKHMAA
                     LIVRIKNHIQVMNPLKENIRTNYFPIYRAVTHFVPTIEEVIGGKILEDEIAFLVIHFS
                     TIASVIKRDLNYVYKSVVVCNHGMATGNLLAENLKEKFPQIEITAVLSSKEIELVDKL
                     DVNLVFSTFQLNYRNKPLLVVDPILTETNILVIEDFLKRNEELQRLEPNSHESTELFT
                     QIIDVMKESGGRVDRIIYGKLEKLFETNNLEINKREIQPMLKDILTDSNIMIKEKATT
                     WEESIELAARPLVKENIVEERYVEAMIRAVNDYGPYIVIGKHMALAHARPEDGVNKLG
                     VSVATIEQPIDFGNPEMDPVKIIFCLAAVDSYSHLNIMKELIELINNEEKLDRLIECT
                     NIEDFKQLLFSVVEK"
     gene            complement(39507..40235)
                     /locus_tag="U2K71_000984"
     CDS             complement(39507..40235)
                     /locus_tag="U2K71_000984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM44-like domain-containing protein"
                     /protein_id="HEM7315088.1"
                     /translation="MGLCLFALPTQGFAVAGGHGGGTSSGRSSGRGSFGGRSYGGSYF
                     GSRSYGRDSYGYYGPSTGPSSYEQTGFLALAVLSVGGVSAFSIVKNKRQKKRLKSEIL
                     SRMSGTAKEKKAQFLEIQKIFMKIQSAWEEETPEKARSCYTDKLFAEHQKVLQKNKAD
                     GVRNHTKKITIQEVGNYRQIHENSFAIRIDFRCCDYSVDRKDGRLISGSKTQRQYFSQ
                     IWYFDFSEKEKRWQADFIQPIMLD"
     gene            complement(40381..43002)
                     /locus_tag="U2K71_000985"
                     /pseudo
     CDS             complement(40381..43002)
                     /locus_tag="U2K71_000985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298916.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IC family P-type ATPase"
     gene            complement(43116..44447)
                     /locus_tag="U2K71_000986"
     CDS             complement(43116..44447)
                     /locus_tag="U2K71_000986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7315089.1"
                     /translation="MEKPAMKRTLTNRNIQLIALGGAIGTGLFLGSAKAIASAGPAVI
                     LVYLICGLAIYGLMRVMGDLFLANSTFNSISDFVDYYLGKKWAFFVNWTYWLCWLGAG
                     LAELTAIGLYIHFWFVHVPALISGGSALMILMAVNLVAVKWFGELESFFSIIKILGIF
                     LFILLGIGLSVSDFHTQSIGIKANFGQLNELVPFGFAGMIASFPMVLFSFAGIEMIGL
                     TVGETSDPQKNLKNAINCLPWRILFFYIGTILSILFVVPWHCLDLKESPLVTMLQVIH
                     CQSGAAIVNLMILVASLSSANSAVYSTSRILYQTAKEKSLPHWFCALSKKGVPLHGIL
                     ITGMLFFVGLCLHFFVADSRLFFQLLAGMTTSCFLFVWSSILCAHVQFIKEKKRNTTQ
                     RYKDLALLVFFTSIAFSLLFESTTRFIPFLLVIWFVFLNFVYKRISNRNIS"
     gene            complement(44413..45891)
                     /locus_tag="U2K71_000987"
     CDS             complement(44413..45891)
                     /locus_tag="U2K71_000987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7315090.1"
                     /translation="MKDTGSSKQITTMTFIGMTCALVASVRNIPDVAATSWTMIFYML
                     VAVLLYAFPISLISGEFAGMFPQKGGPELWVSNALGKKWGFVVSWLLWVQMFPGMVMV
                     ASALAPLFGNIIDNVPLGLNSKFTLVVILVVYWIITFLNLKFDMAKIGGKVGVWLGLY
                     VPLTMMLLLGFAAWIKVGIVPTGTLGSFSWDKLIPDTTTASSFVYFAPIMFIFTGIEM
                     SSVYITRLKNPVKTYIRGVFAALIFIFFVNILNALVVANVVPKGQMELNNIAQSISIY
                     CQILGLPHLIVNLFSLLVFIGVAVQLSAWASGPAKTVTESARKGAYPPKFNFWKTNQF
                     DVSKSVILTQSVIISIFALFYLLIPGVNQAFLMLVNSTTVIYCIVYVIMGIAVLRLRY
                     THAKLNRPFRIGKKEKKSNLGVWTVVIVLFAAIGFSVGLTMKAGTWINLIAVTAISVI
                     LFVVPLCIEKIKKPSWEQEVAMLLEKEKGELDGKTGNETDSN"
     gene            complement(46196..48031)
                     /gene="tdc"
                     /locus_tag="U2K71_000988"
     CDS             complement(46196..48031)
                     /gene="tdc"
                     /locus_tag="U2K71_000988"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEM7315091.1"
                     /translation="MKDMDIKAVFIGDKAENGPVYKMLLNKMVDEHLGWRENYIPSDM
                     PAISEGDKLTPDYLATRDHMIEVLDEVSQRLRAGSIPWHSAGRYWGQMNAETLMPALL
                     AYNYAMLWNPNNVALESSMATSQMEAEVGQDFASLFNMTDGWGHIAADGSIANLEGLW
                     YARCIKSIPLAVKEVLPEQVKKMSEWELLNLSVEEILEMTESFTDEEMDEVKAASSRS
                     GKNIQRLGKWLVPQTKHYSWMKALDICGVGLDQMVAIPVQEDYRMDINALEKTIRELA
                     EQKIPILGVVAVVGTTEEGQVDSVDKIVQLRERLKDEGIYFYLHVDAAYGGYARSLFL
                     NEAGEFVPYASLAEFFEEHHVFHHCVTIDKEVYEGFRAISEADSVTIDPHKMGYVPYA
                     AGGIVIKHKNMRNIISYFAPYVFEKSVKAPDMLGAYILEGSKAGATAAAVWTAHRVLP
                     LNVTGYGQLIGASIEAAQRFREFLDHLTFTVKGKTIEVYPLNHPDFNMVNWVFKEQGC
                     TDLNAINELNEKMFDRSSYMDGDVYGERFITSHTTFTQEDYGDSPIRFVERMGLTKEE
                     WKKEQKITLLRAAIMTPYLNDDRIFNFYTKEIAKAMEKKLNEIIQ"
     gene            48257..>48553
                     /locus_tag="U2K71_000989"
     CDS             48257..>48553
                     /locus_tag="U2K71_000989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315092.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
BASE COUNT        13920 a        10428 c         8111 g        16094 t
ORIGIN      
        1 taaatggctt ttttatttgt ctcgcttaat tatacaaacg gcgtattcaa aagatttatt
       61 tacttttttg tatagatttc gattgttcca cctagtcaat acaaatgagg taatcaggaa
      121 actgaaaaaa atcaatacaa aaaagccgat taagaaatac agcattttct tttctgataa
      181 aaaagcgtac aaggaaaaat ccttggctct ttctggtctc gttaatggga aaaagaccca
      241 tataaaagga agaacaacga tcatccatcc taaaagagcc actaacgttt gccaaatctt
      301 taaccagtaa ttaccttttg aaaaatactt atctttcacc aaaattgatg caacttcttc
      361 tcttgttact tttttcatat cctcacctca cgtttttttg ttacgacgta ggagtttacc
      421 atacatttca tttcttttca cataaagtgt tcctaaattt ttcctaagaa aaagaacaaa
      481 acatttctca tcagcaaatg aaaaatattt tgttcttttt atcagttgtt ctttttattt
      541 tttatctctt aattatgttg ctctttccaa tacgacaatg cttttcattc ctctagatat
      601 cgccgtgtac atttgtctgc gtcttctttg ctcatctcca tcgttttcaa atggatataa
      661 gattacatga tcaaattcca gtccttttgc tagactcaaa ggaatgattt ggcagttttc
      721 taatccatat gaattttttt taatcgattc ttgtagcgtt tgggtttcct cttctgtttg
      781 ggtgataact gccgcttttt ctccttttag agaaaccaaa atctcagcta gtattttcaa
      841 caattcttca gaggatcgaa aagagcggta ttcgggttct tttccttgcg gtcggatcga
      901 tacgatgtcg atcttctctt gatccacagc ataggttttg aaaagctctg tgattgctcc
      961 gctagaacgg taactatttc taagatcata ccgatggatc ggcagatggt agtcatcgaa
     1021 acaacgcata atatcagcaa aagtagtgga agaattaaag attgcctgat tctcgtcacc
     1081 tactaaagta aagtgagttt ttggaaataa ttcgatcaat agacctaatt gtgccagggt
     1141 ataatcttgt acttcatcaa tcaatagata gcgaaatgct ggtacattta ccttttcaac
     1201 aagtaaatga cgaatagcta atataatgac tgcttcatca acagtacgtg tgggttgcca
     1261 agcataagct gagccatatc gtttttcata aattctttca aacaactgat gctcatccac
     1321 ccatgccatt tcttcgattt gtctggttat ttttcgatat tttttttgta aaagtttcct
     1381 tgcataagct acaatacttt gttccgaatc atcagaaatc aatttaccga ataattccaa
     1441 ctgcctatct tctgtcaacg agagtacctg attctgcaca gcaggacttt ttgcttgttt
     1501 caaaagatga ttcttccatt cgctcatcag tgcctttttc gttgcttgaa gcctatcgat
     1561 caatttagga tgagcaggaa ctttctcata tattttttcg atcgtttttt tagaaaggac
     1621 tgtttttcca tttttagtaa gtccacggat aaaattcgga tgatctaaaa acaaaggatc
     1681 tgattgtttt agattatcga taaacttttt gctacgcaaa cgttctgtct gctccgatac
     1741 attttctccc gtgatacgtt tgaaataagc ctcttcacct tctatttcct ctgctgaaag
     1801 ctgtgaaacc agctgtatga tggttagatt cagtggattt ctttcaccta atgatggtag
     1861 gacattcgaa atatagtcga taaaccgatg attaggtgat agaatcagca agtcatcgga
     1921 tgtcatcgta tctcgatata aatagagcag atacgcaata cgctgcatga tagcagaggt
     1981 tttaccactt cctgctacgc catttaccaa gatattttga cttttcacat cccgaatgat
     2041 tgtgttttgt tccgattgga tcgtagcagt gatagccttc atttcattgg tctcatcttg
     2101 gcctaagact tctaataata cgtcatcttg aatagctaca gacgtatcaa aaaacttgac
     2161 caaacgatct ctttccaaaa gaaattgtcg tctttcttta ataaccgtat cgattttatt
     2221 ttgacgaacc gtataagaag atggaccaat catgttattg taaaaaagtt cggcaattgg
     2281 tgaacgccaa tcataaacaa gattttcatc tgcatcatct gtgaaatttg ctgtaccgat
     2341 ataaaaggat tcttttggct cattatccag aaagtctaca acgacttttc caaagtaggg
     2401 acttttcaat aaacgttcga tctttgctag ctgattttct gcgtttttct gtttgatatt
     2461 catctgatcg atttctctat ttttcatttc aatcatcgaa aaagtatcca ggttatctaa
     2521 gtaactgtca aaattcaatc gaagctcgcc agccatttct ttcaacgact ggtttccttc
     2581 tatttttgcc gtatccatcg attcccgcag ttcgtttttt ttatcaatta gcttttgata
     2641 cacgttcttc agatatgctt cttctaactg tttttgtgtg ttgaccatac acattctcct
     2701 ctttttttaa agtggtggaa ccgattagtt tacctctttt aagagtcgaa tgcaagcttt
     2761 ttaacgagta tcatttgtta ggatatatca gttcttcagg tcggacagtt tgatataaag
     2821 aaaatgcacc atcaaggttt tttacgtggt agccagactg cttcaagatt ctttccgcta
     2881 gatagctacg taatccgcta tgacagctga cgatatattc ttgttggctg tctaattcat
     2941 ttaatcggtc ccgaagttca ttcaaaggga tatgtttggc ttccttgaat ttgccctttt
     3001 ccaattcttc tgctgttcgc acatctaata aaatttttcc ttttgctagt tctgtcggca
     3061 attcatgcca ttgtacggtt tcactaagcc cttctgcaat gttcattgct gcatatccta
     3121 gcatgtttac tggatctttg gctgagccaa atggcggtgc atacgtcaat tctaattccg
     3181 gtaaatcgaa gatcgtcaaa tgccctttga ttgctgttgc taaaatatcg atccgtttat
     3241 ccacaccttt tgccccaaca ccttgtgcac cataaatctc gccagtctct ggatggaaga
     3301 tcaacttcag taaaatatct gttgcttctg gataatagct tgcatgatct tttcctgata
     3361 catgaatgac ttcaaaagac agttcgtttt ctttggctgt tctttcactt aaaccggtgg
     3421 atgcggcagt cattccaaac gttcgtataa ttgctgttcc aataccgcct tgatttcttc
     3481 gagaaattcc ggaaatcgtg tccgcaactt gtctaccttg acgattggct ggtgaagcaa
     3541 gagaaataag tgcatctttt ccagtgattt gctgttttac aacgatagca tcccccaccg
     3601 cataaatatc aggaatattc gtttgataac gttcgtctac taggataccc ccgcgtatgc
     3661 ctaattcgat tccagcggtc actgctagct ggttatcagg acgaacacca atggataaaa
     3721 taactaaatc agcagggatg gtacgcccat catccagtcg caatcgtttc ccgtggtctt
     3781 caaatccagc gacagcatga gaagtaatca cttctactcc ttgatgagac aattcttttt
     3841 ctataaaagc tgccatttct tcatccagtg ttggaagaat atgtggtgct tgttcgatca
     3901 ccatgacttc taaacctctt ctttttaggt tttctgccat ttccaatccg atgaatcctg
     3961 caccaacgat cacggcacgc tttggctgtt tttctaatgc atgcatcact ttatcgatat
     4021 ctggtacatt tctaatagaa aaaacattgt cggcttctgt tatccctgga atcgatggaa
     4081 caactggttt tgcaccagga gataaaacca atgcatcgta ctgttcaatg tgtttttgac
     4141 cctcatgtac gacttctacc gttttgtttt cgggaaagat ggctgtcact tcatgatttg
     4201 gacgcacatc taaacaaaaa cgagcggata atgattctgg cgtttggaca agaagatttt
     4261 cccgttcact gatttctccc gaaagataat aaggcaagcc gcaatttgca aaagaaacat
     4321 acggtccttt ttcaaaaaca acgatttctg catcttccat taatcggcga agccgtgtag
     4381 cagcagacat tccacctgct acacctccga caataacgat tttcatctta tttccctcct
     4441 actgttcgat caaaccattg attcatgcca ccacgtacat tcactacatc ataaccagat
     4501 ttctttaatt ctttagctgc ttgtttactc ctcatccctg attgacaaat cacatagacg
     4561 gtcttttctt tgtctccatc atatctgttt atactttgta atggaacatt tttagctcct
     4621 tttatatgcc cgccccggta ttctgcaggt gttctaacat caagtaacgc tggattttcc
     4681 aaaatctttt cctttaattc tgggactgtt atcgatttta ttgattgaaa tagcaaattc
     4741 attttctgca cctcatttac atttttataa atacctcatg gggtatatta aacaggagct
     4801 ttattaatat gtcaaccaga atgactcttc atttttttca ctacttttta tatttgcata
     4861 aaataccgat aaggggtatt atctaattat aaaaatagag gagaatgtgt tatggagatt
     4921 gataataaaa atatcctgaa ccgtttgaaa cgtacagagg gacaaattcg tggtatccaa
     4981 aaaatgatcg aagacgaaaa agaatgtatg gatgtcatca ctcaattaag cgctgttcgt
     5041 tctagtattg atcgcgtaat gggcatgatc gttgcagata atctgaaaaa ttgctttgag
     5101 aaacctgata gtaatccaga agaacaagcg gctaaactag aacaagcgat caagatgatt
     5161 attaaaaagt aaaggaatga tttcaatgaa agtcgttttc catattgatg aattagaaaa
     5221 atggtcagaa acagggaaaa acgtaaaaaa cctgataaaa gcttctccag aaacgacgat
     5281 tgtcgtatct gtaaatggca tcgcgattac cggttatcta gactctgcca atgcagaatt
     5341 cttagatttg caaggagtcg tttttcacgc ttgcgccaat gctatgcgtg ctaatcatat
     5401 ttcagaaagt tctttacctg aacaagtaat cgttgtacca gcaggtgtcc ttgatttagt
     5461 tgaactgcaa tcccaaggtt atgcttatat caagccttag tcagtagagg ttgcgacaga
     5521 agtgatcagc ttcaagaaat ttcggcttat ttttcgaaga agctacttct ggaaaaccgt
     5581 gaatcaaagg ttgtgaaaac gctgtcctgc atcaagtaat aagaacagcc aaacaaaaat
     5641 agcttctcat atttttcgtt tggttgggct tattaccgca gatgcaagct tttgaacacc
     5701 gtttaaggaa gaggttgtga aatcagcggt ttgacctaag tattagagaa aaaaacggga
     5761 aaatagctcc tcatattttg ccgttttttg agctaatacc gaaggtcagc tgattgaaca
     5821 ccgtttacca agaggttgtg agcctaccat ttaactccga atcacaagaa ataatttcaa
     5881 aaacaacttc tcctgttttt ggtttggttg gcttattatc gagaagtagc tactgggagt
     5941 ctatttatca aaaaaaggaa tgcgacatag gtcacacccc ttttttattt tctttgaaac
     6001 atctttcggt actcgacagc atacaagaca aaagattgta aaagaccgta agtgatgacg
     6061 gcaaaaatga ctctcaccca cagcgttggt aattctggga aaagcagcaa aaggatcatg
     6121 ccaacataga tgacagcagt cgctactttg ccataccact tcgctccact taatttgata
     6181 tttttagata gcccgattaa tccagctaca aacataaagc cttcttttat caagaataga
     6241 ccaaacaaca gccaaagcca cggtcgatac catgccatag acaaaatcag aaaaagttgt
     6301 gtcaatttat ctgcaaacgg atcaagcacc ttccctaagt ctgttacctg attatatctc
     6361 cgagcaatga ccccgtctag ataatcagac aaacctgaga caacaatgac cgctcctgct
     6421 atataaaatg actgtctcag cacaaaataa agataaatcg gcagcaaaaa aatacggaat
     6481 atcgataaaa gattcggaat cgtttgtatt tctttacgcc agtctgtgct taatttcatt
     6541 ttatcgccct acctcctcag cactcttttc tttttatttt agcttacacg gggaataaaa
     6601 ccaatgattt cactgttttt atttttcttt acagccactg atattatgag ctaatttgag
     6661 ataatcactt gctgttttca tcgtttactc ttctttcttc ctgtcgtttg cgcatttctt
     6721 ttactagtac tttatgagct ttttttgatc ctcgctctag gtcacgctgc cgtttacgtg
     6781 ttttataatc gataaacaga ctgtctaaat cgtaacctgt cgggtacaga tcttctgctg
     6841 aacgcaccag acgaacacgc tgacgaggca cgtggattat ttttttgtct aaatatacat
     6901 caatcgtatc ttcacctata tcttcataaa ctaacgcttc tttttggtat tcattgacaa
     6961 atatccgatc accttttgca aagagctggc gttctttttt attctccttc ctatttgtaa
     7021 ctgctgtctt aggaagaaat tcttttttct gtgtccgata ggctttttcc gccaagtaac
     7081 gctctgcttg atggatcaat ttcatcgaca tatccatttt atgagcaatc cacaaagctt
     7141 ggctttctcc tgtttctcca acccgcaact gatatttagg acgaagtgct tcccgatcga
     7201 aagccatagc agccggcaca aaatcttcat gatctccggc gaatttcttg atttctccat
     7261 aatgggtcgt ggcaatgatc aatgcccctt gctcatacat agattccatg attgcgatcg
     7321 ctagtgcagc cccttcattt ggctctgttc cactgccaat ctcatctaaa agtaccaacg
     7381 tattcctttt tatctttttc aaaatagcag cgatgttttg catatgacca gaaaatgtac
     7441 tcaacgcatt ttctaaattt tggtggtcac cgacatcgac gaaaatctca tcaaagacag
     7501 caagctctgt cccttctttt gccggaactt gcataccgaa catcgccatc aaagtcaata
     7561 aaccgactgt cttcaacacg actgttttgc ctccggcatt tgctccggtg ataatcaagc
     7621 ctcgatagtc tttaccgatt tcaacatcca atggtaccgc atgctcaggt aaaaatggat
     7681 gtctgccttg cttgataatg atatgttccg atttattgat tctaggtgtg cttccattga
     7741 tttctcggct aaattttgcc cgagcaaaga tgatatctaa caccgtgatc gtttcgatca
     7801 ataaatcgat cacttcttcg ttttctgcga tagcaccagt caatgccgcc aggacttgat
     7861 attcttcact gatttcttca gcttttaaca actgatagtg ttcattcaat ttagaaatca
     7921 cagttggttc aataaaaacc gtcgttcttt tatttgattc atcgataatc gttccatcta
     7981 ttttattttt atagctggct ttgataggaa tcgtatagta ttcgcctttt tgaacaatca
     8041 ttgcttcttg gatcatttct ttgtttttcg gatgacgcaa gaatttcagt aattttgact
     8101 ggatttcttt ttcgattagt tgtaattgtt tgcgtacttt acgcaaatta cgtgacgcgt
     8161 cgtcactgac tttattattt tttattttct ggtcaatcaa ctcttctaca tttatcaaat
     8221 caggaagatg tttgctataa gcaaacaaca agggtgtctg gtattgattc ttatcaaaaa
     8281 actttgtgat catccgactg cttctaagaa aatccgcata ttcgatcaaa tcggttggct
     8341 gcagaatcaa cccttttttg acttgttctg tcagtgcatc gatcctcggc aaccccataa
     8401 atggaacatg ctgactgctt tctaagatca agcgtgcttc tcttgtttct tcttgacgat
     8461 ccaagacagt ttgtaggttt gtcgagaccg tcatatcgct gattcgtttt ttgctatagt
     8521 ttccaattgc tcttgcttgg atttcctttt tgatccgatc aaattgtaat tgactaatca
     8581 tttcgttgtc catattctct ccttttcttg aagagatacc tgtcccaaaa taaataaggg
     8641 cctagagata aataaaagga tagaacacag cttaaaaaag tcatgtccta tccttattag
     8701 aatacaaaga tattgttttc ctgacaggta ccgtttcata agcacaccaa ataaactttg
     8761 tttattggct attcgactta ctgattagat accagctaca ctcacaaaac ataccccatt
     8821 cttctaggtc ttatttattt tccacttaac tatagcaaga cacattttgg ttgtcaaact
     8881 ttagtatgtt ttatagattt acaccattaa tgaatagaca cctaccgcga taattgctcc
     8941 gatcattgga cctgcgattg gtacccatga atatgcccaa tctgaagttc ctttattagc
     9001 tattggcaat accgcatgtg ctaatcgagg accaaagtcc cgagcagggt tgattgcata
     9061 tcctgttggc cctcctaatg aaagaccgat ggctaagatc aatccaccta cagcgaatac
     9121 gttcgttcca ggtgcaaatt cgttttgtcc aaaagctagc aacccaagaa ctaatacaaa
     9181 tgtaccaatt aattctgtga tcacatttgc tggataattc cgtatagcag gacctgttgc
     9241 aaacgttcct aaaatcgcgc tttcatcttt cgttgcattc caatgtggca aataagaaag
     9301 ccaaacaaga atggcccctg caaaagctcc taaaacttgc gcaatgatga aaggaacgac
     9361 aagattccaa ctaattgccc ctgtcatcgc catagctaga gagactgcag gattcaaatg
     9421 agctgggccc atgaatccag aaatatagac agccattgtt acagcgagtc cccaaccaaa
     9481 tgcgatgaca atccagccag aagcctgtgc tttacttttg tttaaattca cagccgcaca
     9541 cacaccatct ccaagcaaaa ctaggatcag tgtcccaata aactccccca tgatttgagt
     9601 catatcactg ttcatttatt tctccccctt tagttcccgc agatctgatt ctgcaatcaa
     9661 tgcttctaaa cgtttagtct gttgcgcctt ctcctcttct gtccattcaa aatatccagc
     9721 aattgcatcg atcacaggtt gcttgatctc atcaagctga tctcgttcaa acaacagatg
     9781 atttgtacga cgaatgagat aatcgcctgg ggccaaaacc atttcttctt ctaaaccata
     9841 acgtaaccga gctgactcag cgagactcaa accaggataa ggtgccattt cttttgctaa
     9901 ttcaaagata cgtcgggcat tagtcccgta aaaatcagcg atataagtag catcttcttc
     9961 ctctaaaccg gctgctactc cttcttttgc taattctgtc actgtttctt ctaatttagt
    10021 tggatcgaag tttccacctg aaatctgata ttttttagag tcgatttctt tcgtctctat
    10081 tccgtattct tcttttaata attggcgtat caatcgcaaa gctccttctg ccatcttacg
    10141 gtaatcggtg attttcccac cagataaagt cagcaaacca tctggttctc tttctaaaga
    10201 actacctctg gaaatcgtag aaggtgcttt ttcatcacgg ctgttttcca aatggttcaa
    10261 cacgtcttct acttcagcac gagaaacttt gttttcctta tattcactta ctgtatcgac
    10321 aactttattg aagctcttgt ctgaaatcga tccattatct ccaccattgt aatcagaacc
    10381 agaattacca atcaacagtg gacgaagtcc tgcccagctt gcttcgatat cattcaatgt
    10441 gatatttgct tctggatagc gatgattgat cacatccaat agataatcta catcttcttg
    10501 tgtgactttc ggatccgtaa aatctccttg gtaatccgta tctgtcgtac caaagtaagt
    10561 cttgttttct cttggaatag caaaaaccat ccgcccatct tgctttcctg tatcgaagta
    10621 tgtgggttgc ggtacaggca gttttttcgc atctacaact aaatggatcc ctttagttgg
    10681 acgcatttta ggagagactg gacgcgtaaa attcaagttt cgtactttat ctacccaagg
    10741 accactcgta ttgatcacta attttgcttt gatttcgatc acttcatctg tcagcaggtc
    10801 acgagctttg acgccaacga tttgatcgcc atcatataaa aagccaaccg ctttcatttt
    10861 acttactaga taagccccat cttcggcagc ctttttgata ttatcaatca ctaaacgggc
    10921 atcattgttg cggaaatcta gatagacacc cgcgcctttt agcccttctt ttttcaaaaa
    10981 cggttctctt tccaatactt cttctggtgt cagcgtataa ttctcacatt tggttcctgt
    11041 cacatttgcc agtttgtcat aaaggtccat tgctactttg accgagaaca tattgaaggt
    11101 tgttgctcct tcgccctcat agattggcaa aagcattgga tctggttttg ggatgtgtgg
    11161 ggcaatactt tgtacgactg cacgttcacc aactgtgtca gctactactt ccacatcaaa
    11221 tgtcttcaga taacgaatac cgccatgaac aagtttggtc gagcgagagg aagtcccttc
    11281 tgcaaaatcc tgcatttcga tcaatcctgt tttgattcct gatgctgctg actggactgc
    11341 cacaccggca ccagtgattc cgccaccgat gatcaacaga tccaattctt cttgctgcat
    11401 tttttgaatg ctatcttgtc ttgtcttgtt tgaaaacata tggatttcct ccttattcaa
    11461 atgcttgggt agcttttact gcttttttcc aaccgttgta aagtttttct cggcgctctt
    11521 cctccatttg aacttcaaat agcttgccag cttcataaaa ttcgcggatc tcgtctgtat
    11581 ctttccaata accgacagct aatcctgcta aaaatgctgc ccctagcgcc gttgtttcca
    11641 agttttctgc tcgttggatc ggtacattca agatatctgt ttggaattgc atcagatatt
    11701 cattgtttgc tgcaccgcca tctactttca atacaggaat cttgattcca gtgtcatctt
    11761 gcatcgtatc gatgatatct ctcacttgat aagcaatcga ctgtaaagta gctttaatga
    11821 tatcttcttt tgtcgtacca cgtgtcaagc caaacatagc ccctcgtgct gcctgatccc
    11881 aataaggagc acctagccca acgaatgcag gaacaacata aacctcatct tcgcttgtag
    11941 agcgtttcgc tgcgtcttct gaatcgctag ctttttgcaa catttgaagt ccatctctca
    12001 gccattgaat cgaagaacca gccacaaaaa tacttccttc taaagcatag taaacctttc
    12061 catttatgcc gtaaccaatt gtcgtcaata aattattttt ggataattgc ggtttttcac
    12121 cggtattcat gacaataaat gaacctgtac cataagtatt cttgaccatg cctggttcga
    12181 atgccatttg tccgaataat gcggcttgct ggtctcctgc catccctgaa attggtgctt
    12241 cgcttccata aaaatgataa ccttgcgtat aaccataaac ttctgagtta gaagtgactt
    12301 ttggtaacat cactcgagga atatttagta aatctaaaat ctcctgatcc caatctagct
    12361 gatggatatt gaacaacatc gtccgactgg cattcgagta atcagtaaca tgtgcttgtc
    12421 catcggtcag tttccatact aaccaagaat cgatcgttcc aaacatcaat tcgccttttt
    12481 cagctcgttc ttgcgcgcct tctacatgat caagaatcca gcgtatcttc gttgctgaga
    12541 aataggcatc gatcactaac cctgtttttt cgtggaaaaa ttcttgatag ccttcatttt
    12601 ttaagttatc tgcaattcca gctgactgtc tggattgcca aacaactgca tgatagattg
    12661 gacgtcctgt atctttttcc catattactg ttgtttcacg ctggttcgtt atgccaatcc
    12721 cagcaatctg atttggtttg atccctgatt cgatgaacgc tccggcaata actgactgta
    12781 cagaattcca gatttcattt gcatcatgtt ccacccaact ttcttgcggg aaatattgcg
    12841 tgaactcttt ttgagaactg ccgatgtgtc ttcctttctt gtcaaagata attgctcgtg
    12901 agctagttgt accttggtca atcgccatga tatacgttga ttctgtcatt gtgattccct
    12961 ccattatatg caaacgcttt ctttaactac tcttatcata ctactgtttt aaaaaaagaa
    13021 actgtcaaaa ttttattctt tttttctttt tttgacagtt ttttattatg aagctatttt
    13081 ttaatcaatt cttttctttt ttcagcgtct ctattcttct tttcaatcga ataatgtcgt
    13141 aaggtgacgc aaattctgac agataatatt cacactgttt tttgtttctc tggcaaaatg
    13201 tgatcaccaa atcatattct ttaccttctt cataacttcc aatctcaata ccgccgattc
    13261 ccctcagttc tctgatcaga tattgttgaa aagcaatacg ataaatcggc agactttcta
    13321 aatcgattcc aatcactact ggttttgcaa tataaaaatc gatcatcaaa agaacattgg
    13381 catactggat catcaggtaa ctaagctcac tatcttcagc tcttttcctc ggcaattgct
    13441 gcaccgctgt ttcctgtaat ttcgccagta actgtgttaa ggaatgcccg agcattttct
    13501 gctgctgttc caaaagccga tcacgctcat agacttcgat ctttccaaca aagaaaaacc
    13561 attcattgtt gatctgggaa atttggtagc ctactgcctt ttcttctttg attttcaaac
    13621 gacgaggacg ataatgccat aacatcgttt cccgaatgta agtatctaaa acagcggttg
    13681 gtaacttttt gctccgtgtc aaatcatact gataaaatat ttcttcgtct actatcgaaa
    13741 aagaaacaaa aaagctgtaa aacatcattg tttcgtatac attcagttct gctgctgttc
    13801 gactaagatg catcacgatg attggatcga ttgcttgata aagccggtcc gactgataga
    13861 gtaatttctt ttctttcaaa gttgcgtacg tacttttttc attcaatagt ctttttttcg
    13921 taatcccgag ccagcaagca atctgtgctt ctgctgatgc cgtaaacgtt gtttctaata
    13981 cacggtccaa tcctagaatg aacggtcttt tttctggtgt attttgcaaa aaaagcggtc
    14041 gctgatcttc cgggatatat tggaatagtt cataataaaa atatcgtatc tgtagctctt
    14101 ctccgtataa ttgaccgttt ttgatttgta atccaaattc ctctaagagc tggttcaatt
    14161 ctttgatctt tcgaaaaaca gaagactcac tgatattaaa cgctgtcgct aatcgaacga
    14221 tggatacttg ctgatgttct aataaataga cgagcatctt aaacttcaag ctttcctgca
    14281 ataaaaacaa tagaattttt tccaaactta catcttcgtc caacacaagc agcgcctgat
    14341 actcatttcg ctgtacttct actttttttt ccatcattcg gccaatcaat tcaatatctt
    14401 ccaaatattg atcaaacgag gcttttgaca gttggacatg ctctctcaaa ccattgatag
    14461 aggctgttcc cccttgcaga acaaggtttc gcaaaatcag gatctgtaac tgctcttttc
    14521 tttctaataa tgcttcaatc ttcataggca gtattcgcca cctttgacca caccatatct
    14581 ggcacacggt attctggctg gaaaaatagc tcatgttctt ctaactgttc aaacgtccat
    14641 ccttctttta ctgcaattgt cagttggtca agaaggtgga acatctcttt tttgctttgg
    14701 agctgtgctc caagaatctt tttttcagaa tcaaatagaa atttgagttt aactttgctt
    14761 tgatttattg ctgtaagctt gtagtctacg tatcctgttt caattttttg gggataaaaa
    14821 atgctctctt cttctgtcaa acctacgctt cctaagaaat agccaaacca ctgattgccg
    14881 actggacgat aggttccctg atcctttgtc tgtgagccac taattgtctt gctgacattt
    14941 cgtgctgtac ggacagcgtt atttactaga gaaacataca ttttttcatt tgttggacga
    15001 aaagttactt ggatcgcatc gccaatcgca taaacgtctt tttgagacgt ttgcaagtag
    15061 tcatctaccc agatcgttcc atcatcattc aatgttaatt gttcttccca catctgattg
    15121 tcggggcgag aatgattagc cagcaaaaca agatcatagt tctcctttct tttttccgtc
    15181 tctacaatag cggtttcatt ttcggtcaaa gacacaactt gttcttctaa aaaaagtgaa
    15241 agcccttgat ttttcatttc ctttactaac ggttctgtca tttcaggatc tagataacga
    15301 aaaagaattg actttcggct ttcgtaaaga tggatttgtt tttttcttga tgccaaccct
    15361 tcagctattt cgattcctgc ttgtcctgca ccaataattg caattttctt tgcattcatg
    15421 atcttttgct gaagagccgt tacgttttgt atctttttaa ctgggtgaat aagcgttgat
    15481 tctgcagaaa tatttcgaaa cttttgattt gaaccagtcg ctagaatcaa acgatcaaaa
    15541 tccaattttt ttcctcctgc taaaacgact tttttttctt tcaatcctat tactgtcttt
    15601 tctgtgtaaa catggattcg atagcggtca gtcagttctt ctttcgtgat ccaagaaagt
    15661 tgtttttcac tggtaacctt tccttttaat atcaaattga acccgctggg aatccatcct
    15721 aacttcgatt gcttttcaat caaatatatt tcacttgttg gcatcaattg gcgaagataa
    15781 atagccgcat gaatccctgc aaacgacccg ccgacaatta caatgttcat ctgcattcct
    15841 ctttcttata aggtgttcta ctgctaactt ctcaatcaga aaacacagta ttcataactc
    15901 gctttcaacc ttatgataca tagcgatgca gcagaatcaa ccctctccat cataccgtgt
    15961 tctaggtctg cttctcaata atgtactagt ggacctaatt gaagagtatc atttctgaag
    16021 agatgtttca aaaaatgtac tcttataaaa gaaaaaaact cgccttttac gtacctctgt
    16081 ccaaatagga aacagaaaag aacgaaaaga cgagttttag cgtgtgtaat gtaatagtta
    16141 gaatagacac atcatttatt tttaattctt taatgcttca acaactgctt cgcctaattg
    16201 atgagcagat tgaggatttt gccctgtaag aagccgccca tcaatttgaa ccacttctga
    16261 aaaagctgct ccagactgat agtttgctcc tttttctttc aaagcatctt ctgctaagaa
    16321 tggtacttca ttcgttgtac catttgcttc ctcttcttca ttactgaacc cagtcactgt
    16381 acgtccagct aaaatagatt ttccagactc atctgtaact ggcaacaatc ctacaacacc
    16441 gtgacaaaca gctgagacca gcccttcatt tcgatagatt tgtgaagcga ctttcccaag
    16501 atctattgct tctggaaaat cccacattgt tccgtggcca ccagcataat aaaccgctgc
    16561 ataatcattt gcatccacat catcaggacg taaggtattt cccaaggccc gatttctgaa
    16621 atcttcatct tcataatagg cccaatcgat ttcatccata ttcaaaacac tttgcggttc
    16681 taaaggtaca tatccgcctt taggactgac aaaatctgca tcgatcccat tttttactaa
    16741 tacatcataa aaatgagtca gttcacttaa ccacaaacct gtaggacgct cgatcttttc
    16801 gtacttttct acatttgtta atacgatcaa tactttcatt ttcccatcct ctttctataa
    16861 aaattgcgtt attgataacg tgctttcaag aaattatata atgggcttgg caaataaagc
    16921 tctttatatg cttcttcaaa aggtttcgtt acaaattcat cctcacggtt ttccaataat
    16981 ttctctgccc agtcaggatc gacgatcatt tgctgtccaa ttgctgcaag ttctgcatat
    17041 ttcaatacct gctctacatc ttcgcgattg cggatcccac cgacaccaac taaagggatc
    17101 tttccttgaa tcgtgttatg gaccacttca agaatcgatt tatcgttgta tttatcagaa
    17161 cgagctattc gatcatacat atttaatgaa acatgcagat aatctagttt tgactcacgt
    17221 aatcgttcaa gaagccagat cgtgtcttca aaacggatac ctggtgtttc atattcttct
    17281 ggtgagaaac gataccctac gatgaatggt tttttagcat attgttcaac cgctgagaaa
    17341 accgttgtta ataattcttc aataaaacga taacgatttt ccaagctacc gccccattga
    17401 tcctctcgtc tattcgaatg cggagagaaa aattgttgga tcaaatacgt atttgctcca
    17461 tgcaactcga ttccatcaaa tcctgattca atcgctctac gtgtggcttt gccaaatgct
    17521 tggattgttt caacgatttc ttcttcactc attgctcgtg gtgtttccgc atcaggacgt
    17581 tttgctgcaa ctgcacttgc agatacaggc tgttctccag ataaagttgc tcgacttgtc
    17641 attcgtccac cgtggaaaat ctgaataatc gcttttgcgc cttctccttg gattgctttc
    17701 gctagctcac ttaatcgagg aatcatttca tcatgagcga tgctgagttc acctggccag
    17761 ccttttccga tatcttggac atttgcagca ccagtaataa cagcaccaag acctttagaa
    17821 cgttgtttat aataagtgat ctcatcttcg gtgatcactc cattgaaaaa gctgagttgt
    17881 gtcgtcatag gcgacagcat caatcggttc ttcaattgaa ctcctgaagg taaagtaatt
    17941 gattgatcaa attgtgacat tgtcattcct cccataattc gctctgatta acctatcgag
    18001 aatcatctat cattaaacca ctcgattagt tgtacatgca actaatttgc ttaaaaaatt
    18061 ttattttttt tgtaacttat ctgttgcatc attcattgtc tgggctaatt ctttacttgc
    18121 ttcatttccc aaaactgctt tgtatgactg atgaagttct tcccagctac catcgacttc
    18181 cttagcaaat tggcgtcctt gttctgtaag ataaactaat gacaagcgac cttcgctaac
    18241 tgttttgacg tatcctttat attctaattt ttcaacaaaa cgagcaatcg ttgatggggc
    18301 ggaatagagc atttgtgcaa tatctttttg tggtattcca tctttttctt ttaatatcaa
    18361 taaaataaaa ccataagttg gtgccagacc tgtccttcta aacgcttcat cagctaattt
    18421 attcaacatg cgatctagct ttttcacgct aaagaaaaga cattcatcta acgctttttc
    18481 aattggcttt tccatttcat cccctccatt ggcgtacata tcatagtatg atttttattt
    18541 tttgtcaaaa aacacggaat aatgtccgcc tcctctgtct agatttgaaa aaaagaagta
    18601 gccatcttca aagaatttcg gcttgttttc gacaaaactc cttctggaac accgtgaatc
    18661 agaggttgtg aaaaagtcgt ttagttttga gtatgaagaa aaattttgtg aaaatggctt
    18721 tccacatttt tgcataaatt tggcttaata ccgaaaaact ggcttttgaa caccgtttat
    18781 atcagaggtt gtgaaaaagc cgtcctgcat caagtaataa gaacagccaa acaaaaatag
    18841 ttcctcatat tttgtcgttt tttgagctaa tgccgatggt cagctgattg tacaccgttt
    18901 attgagaggt cgtgagcctg ccgttgaact ccgagtcaca aggagcaatt tcaaaaaaca
    18961 gcttctcatg ttttatgact atggtgactt gtgatcgagg agttggcagg agaatactgt
    19021 ttattcgtaa agaaggggct gtgacaagac ttttgtcaca gtcccttatt tccttttatt
    19081 ataggatttc gccatttgat tggatgactt gtctgtacca atcaaatgat tttttcttta
    19141 accgtttctt tgtgccattc ccttcgtcat cttgatcaac atagatatat ccataacgtt
    19201 ttgacatttg tgaagtagaa gcgctgacga tatcaatgca gccccacgac gtataaccaa
    19261 ataaatctac tccatcttcg atagcttcct tcatctgctc aatatgctgc ttcaaatagt
    19321 caatgcggta atcgtcttga acggttccat cttcttctac tttgtctata gctccgagtc
    19381 cgttttcagc tatgaacagt ggtttttgat acctatcata taattgattc aaagcaattc
    19441 tcaaaccctt aggatcaatc tgccagcccc attcacttga tggaagatac ggatttttaa
    19501 ttcctccagt tattaagttt ccttggaccc cttccaaatt ttctggatga gcactgactg
    19561 cactagacat gtagtagcta aaagaaacaa aatcaacagg gtaggctgct aagatttctt
    19621 catcacctac ttctattttt aagttgacac ctttttcttc aaacattcgt tgcgtatacg
    19681 ctggataata tcctcttgct tgaacatcac taaagaacaa agaagttctt tgctgctcaa
    19741 ttgttttttg aatatcatcc ggatcacaag aatacggaac aagaagctga tagctgatca
    19801 tacaaccgac ttgtgaacct ttgataattt catgacaagc ttttgtagct aaagcacttg
    19861 ctataaactg atggtgtgca ccttggtatt tcgcttgttc caaatgggga tggttttctt
    19921 caattactcc tacgctaaca aaagggtgat gttttacaca gttgatctca ttgaaagtca
    19981 tccaatattt tactttattt ttatagcgtt tgaatactgt ttcagcgaaa cgaacgtaaa
    20041 actgaataag ctttcgatta ggccatccac catattctgt aaccaaatat aacggcattt
    20101 cataatggga aagagaaacg actggctcaa tcccatattt tagacattcg tcaaacactt
    20161 gatcataaaa aaataatccc gcttcattgg gtgtttcttc atcaccatga ggaaaaattc
    20221 ttgtccatgc gatagacatc cgatagcatt taaaacccat ttcagcaaat aaacggatgt
    20281 cttctttaaa gtgatgatag aaatcatttc cgtgtctttt aggataataa tacttgctgt
    20341 tgggatcctg cgccgctttt acttgctgat gcgtcatcat atgccattga tctgtccatt
    20401 tttctttagg taaatcgctg tcgaatgtat acacatcaga cacagataat cctttttcat
    20461 ctacatccca tgcaccttca cattgattgg ccgctgtcgc tccgccccat aaaaatcctt
    20521 ctggaaattt tttcatatcg atccccctaa acttgctagt tttcttgttt gattattttt
    20581 cttatgacta gtaatgatat ttttgaatct actactattt gtaacaataa ccattgtggt
    20641 cggatcatat ccctctttga ttaattcttg caaatctact tccatcaata aatcgccttt
    20701 acgtactttt tttccatcag atactgcact ttgaaagtat ttgccattta aattcactgt
    20761 atcaataccg atatggatca gtatctctgc tccttcttca ctgactaccc caattgcatg
    20821 tttcgttgga aaaactgttg aaacttcccc gtttacagga ctgtagattt ttccgtccgt
    20881 tggaatgatc gcaaaccctt ctcctaaaga tccactagca aatgttggat cgtttacttc
    20941 agataaatca attatttcac ctgctgcagg agtacttaac actatatcct ttgaggattc
    21001 ttcttcgatt tcatccttgt agccgaaaag aaaagttagc atacaggaac tgatcatcgc
    21061 taagaaataa ctagcaacta gccaaacaaa tccttcacca atataaatcg ggaaagacaa
    21121 aatacttctt gtagcaactg ccaaagcacc ggctcctgcg gcaccagcta ctccaccaaa
    21181 tatagctcca catatacaag caatgtaaaa tggtcgttta tatttaagag tcacaccata
    21241 aacagcaggt tcagtgattc caaataatgc tgtgataaat gcagataaag ataaactttt
    21301 tactttctta tttttcgttt tcaaaaatac cccaagtact gctcctgctt gcgcagctac
    21361 ggcagtcata catgctggac ctagcgtatc acgaccgtag aaagaaagat tgttggtcat
    21421 tactggtaca agtccccaat gtaacccaaa aatcactaac acttgccaaa atccgccgat
    21481 aacagcacca gataaaacag ggctaaagtt ataaacagct aaatatccag ctgcaatcag
    21541 ctgacttgca taatctgcaa ttggacctac aactaataaa gtaattggga ccatcacgat
    21601 gatacaaatg aatggtgtca ataaatttcg aatactttcg tgcaagcttt tgcgaatctt
    21661 aggctcaata taagaaagaa cccatacagc taaaattgct ggaataaccg tacttgtata
    21721 tcttgctaga atcacgggca tccctagaaa tcgcaaggtt atgctatcac tatatgcact
    21781 gacaatcgta ggataaacga gagctgctgc aataaccata gcgatgaaac gatctgtatt
    21841 gaactttttt gcagcagtat atgccaaaaa tataggcaag aaatagaaaa atgcatcagc
    21901 tgctgcattc aaaatgatat aagttcctga ttcagtagtt aataaattca aactcgtaca
    21961 taaggcgagt actcctttga tcaaaccact tcctgccaag acgcctaaag tcggggcaaa
    22021 aacagcggcg atcatgtcta taaaagagtt taaaatgcct tttttctcag ttcctctctt
    22081 tccactagat gcatcatctt ctagtttcat ttgtttggat aatacgtcat acacttcatt
    22141 tactgcattt cctactacga cttgaaacat tcctccagct tcgactacag tgatcacacc
    22201 ttctagttgc tctaccttct ccttatctgc cttcttatta tcttttaggg taaagcgtaa
    22261 ccttgttgca caatggtaaa cattgctgac attttcttcg cctccgacaa atcggacaat
    22321 atctttcgct aattgtgaat agtccatttt tacttccccc ttgttaatta atgccgtagc
    22381 aatccttctt acaagataag tatagttctt agaaaacggt ttctccattc aattagtacc
    22441 ccttctaata gcgaacgaaa ctgtacaata gtgtgtatgg tttacttagc cgagcaaatc
    22501 actcactgct tgaaagaaaa caagtatatc gtttctctta tagtcaaagg agacaaaaaa
    22561 aacacgcttg atcttccttt gctgatgtat aagtggtatc tcaagctgat actctgtgat
    22621 ttttgtttct acttggcttt tgataagaca gcacaacaaa gtctcggcat ttaacggaag
    22681 cataactagg tcagaactag gagagattag ttgctgaatg gaatctatct ttttatggat
    22741 ttttatttga ttttcttctt tgtagtaatg atcaagcagt tttgttttct ttttttcttc
    22801 aactatggtc agcttttcac gcaaaatctg ataaatctcc gaagattcaa actcatctag
    22861 aacactaggt agataaatca aatcatccca ctcaaaaata agctcttgct cagtagtcaa
    22921 aattgcatca tattccgtta ttttgtttga ttgatttttg aaaagataca ctggttctat
    22981 ccggcattct ttgatccaca tgttcatact ttgttgaatc tgttttttta gtgtattcaa
    23041 aatactaatt ggctggttac taacaatcag tatctcccct tgttttcgaa aaggattcaa
    23101 cgcttcggca agatacaaaa ccaaataagc gctttcagct aaattgggac gtaattgaaa
    23161 atgtattggg aagaaacgcc gaattaaata aatttctaaa ggataacggg agatgatctt
    23221 ttgtacaaaa tgattcatgg tattatgtcc ggcatccatc ctcgttttca tttgtttgat
    23281 atgggttagc aaaaattctg tttcatggaa caataacgaa acaggaagat ctaaaaaacg
    23341 aataatcgtt tgctcaaatg ctcttagatt cttctctatt tctttgtttt tttcatcatt
    23401 aatcatcaaa taatttaact ctaatagtcg attttcaatt gcttgttttt cttgattgga
    23461 aaaagaatag ggtagttctg atgataactt ttttgtgagg gtctctacca tcggaataaa
    23521 taatgtatac ctgtttgttt tttctaaaag ttcttctctt cgaagaatgg tcaaaacgag
    23581 ataccgagaa aatcgtatga agtcctctcc tgaaacaata taattatatg atataagaac
    23641 ctcttttaat gtcttttgga cagtcggcat gatattggaa aaaattgtac tgatttgttg
    23701 aggcaacctt gcgtgttgga tcaccgtttc tgttccaaca agagataaga aaattctttt
    23761 tgaattttct tcagcaatga tttttagtcc ttttgtcgac gaaacttcca gttctaattt
    23821 aggattgcgc tcgatccaac gcaaaatcgt ttttatttgt aatgatacgg ctgtttttga
    23881 aagaaagaaa atgtctgcta tcttttgcat actgacatac cctttttgaa acaataagta
    23941 cgtcacaata gttgaagctc ggaaatccat gctggaatag attttatgtt tcgtcacctc
    24001 ttgtatgatt tgctgtttag tttcagattc aattgattct aaaaaataac ctgaacgttt
    24061 atttgactgg ataatgattt tcttctcgta gatagcaagc atatttttga tttcagtttg
    24121 gatcgttttc ttgttggtac ctaaagcttc agctaattct tcgcttgtta tccaatgatt
    24181 cttatttctt aatactacaa gaatttggag ttgtcgttta tttaagtcca tttgcttaca
    24241 cctcctcttt gttataaaca tacaggaaaa acctctcaaa tgacaatagt cacttgcaga
    24301 ggtttaaaaa tcatttgaag aaagatctat attcaaggtt tttaccatta gactggataa
    24361 tttgtttata atattcaaaa ctatcttttt tcaatctcct aaagcttccg ctcccatcat
    24421 tttgtctatc cacataaata aagccatatc gcttcctcat ttcccctgtt cctgccgata
    24481 cgatatcgat tggtccccac cataagtaac caatgatttg gcaaccatct tttattgcct
    24541 cagcaatttc ctgaagatgt atttttagat actcacaacg ttttggatcg tggattcgat
    24601 tcaattcatc tggtgcttca tctagtccaa tgccattttc aactatgaat aatggtaatt
    24661 cataacggtc tgtcaattca ttcatgacat aacgaaaccc tagaggatca attggccaac
    24721 tccaaggttc gggagatttt tccttcaaaa agggattatc gaaaccattt gctccgccag
    24781 tatctacgcg catttctgct tcttttttat agacagcgct acgataataa ctaaatgcaa
    24841 tataatctac cgtgttagct tgcaagtact gttcgtctcc atctaacatt actggtgcac
    24901 agtcttcttc cttccatatc ctgtaaatat aagctggata atgccctcgg cacatgacat
    24961 cggagaaaaa ccatgaattg cgtttaaatt gcagtgaacc aaatacattc tctggatcac
    25021 agtcatatgc ataagtcgct aatgcagaaa agctacacat aatacccatc ttagcatttg
    25081 gatcgatttt ctttaataat tgagtagcac gggcgttcgc tagaaacata tgatgacagg
    25141 cttgataaat ctcttttttt gttagtgtag caatgggttc aagtttatct ctagcaggga
    25201 atgtaaccaa tccagcagct gcataaggca tacgaaaagc attattgatc tcattaaagg
    25261 tcatccaata tttcactttc ccttggtagc gttcaaagac ggtctgcgca taaaattcaa
    25321 aaaagtcgat taattttcga ttttgccacc ccccatactc tcctaccaat gctaaggggg
    25381 tttcataatg actaagagta attactggct cgatatcata tcgatttaat gtattgatta
    25441 gtctgtcata aaatacgaga ccagcctcat ttggcttctg ttcgtctcct cttggaaata
    25501 ttcgtgccca tgagatgctt gttcgaaaag cttttaaacc taactccttc agtaaacgaa
    25561 tatcctcttc aaatcgatga taaaaatcaa tcgcttcatg agaaagataa tggagtgatt
    25621 cgtctaaagc aatctcccaa atcttttttt cttcattcca ctgaatacta ggatgctggt
    25681 tcatgatccc gataagcgta tctgtcacat taggtaattt gccgtcttcc aaccatgcac
    25741 cttcacattg attagcagct actgctccgc cccataaaaa attttctgga aatatttcct
    25801 gatccataac tatctctcct cgctatcaac aatttcttac ttgaagtata gctcgattca
    25861 tcaaaacaag atattctaac tatttcggca gaagagaaga aaaaaattga atgctactct
    25921 tcatttttaa tatttatata taacaaatac ataagacgat ccccgaaaga aaacactcgt
    25981 taaccatcta agcaaacgat ttattttact tccaacacaa atgcttcata aggtcgtaaa
    26041 tcaactttgc cgtctggtaa ctgtttcaga ggataatttg acacaataac ccttaaagct
    26101 tcacgtggaa cagtgaacgt ctgcttttca ctggacatat tacaaataac gagccattgc
    26161 tggtcttcat atatgcgaga ataggcaaag acatgctcag gcgtcttttc cactaattca
    26221 tagtctcccc agacgatgat gtcatgtttt ctacgcaaat cgatcaattt cttgtacata
    26281 taaaaaacag aatcttgatc tgctaacgct tcttctgcat tgatttcttt ataatttgga
    26341 ttgactcgaa gccatggaat tccagtagta aacccagcat gttcacgggc gttccattgc
    26401 ataggcgtcc tagcattgtc tcttcctttc gcattgattg attccaagat ttcctctttt
    26461 gtaaatccag aagaaatcct ttcgttatac atattgattg tctcaatatc ttttgcttcc
    26521 gcgatatcat cgattgggca attcgtcatg cctaactctt ctccttgata gatataaggg
    26581 gtgcctttca tcatatggag caagatagca aacatcttgg cactttcttt tcgatacaca
    26641 tcgtcatttc cccaacgaga aacaattcgc ggaagatcgt gattattcca aaataaacta
    26701 ttccatcctt catcacctaa agatgtttgc catttggata atactttttt cagttcagag
    26761 atactcaagg gtttcaaatc ccatttgtct ttgccttctt gctgatctaa tcctatgtgt
    26821 tcgaattgga agaccatgga taattcattt cttttaggat tggaataaag tttagcgatt
    26881 tcggtagttg ctccccatgt ttcgccaaca gtcatcagat ttttgtcacc aaaagttgct
    26941 tgattcattt cctgaagata ctcatgcagt ttaggtccat tacttgtgat ttctttatcc
    27001 ggttctttgc caatcatatc gatcacgtcc atacgaaagc caccaatacc tttatcgatc
    27061 cagaaattca tcatttgata aatctcttgg cgaacctgtt tatttttcca attcaaatca
    27121 ggttgacgct tgctatacaa gtgcagaaaa tattgccccg ttttttcatc caactgccaa
    27181 gcagaaccac tgaatattga gcgcaatcta ttaggctctt ttccattgac cggatcacgc
    27241 caaatataat agtcccgata aggattttct ttatttttac gggcctcaat gaaccacgga
    27301 tgttcatcag aagtatgatt caccaccaga tccatgatga tacggatatt tcttttcttt
    27361 gcttcttcaa tcagttcttc catttcttcc atcgtaccga attctgccat gattgcttga
    27421 taattactga tatcataccc attgtcatca ttcggagact cgtatactgg agaaagccaa
    27481 attgcatcaa tacctaaata cgccagataa tccaacctgg aaataatccc ccgcaaatct
    27541 ccaataccat cttgatttga atcttgaaaa cttcttggat agatttgata aacaactgaa
    27601 tggtgccacc atttttgttc catgattttc ctcctataaa tggatgtttc tattttttgc
    27661 ttaatcattt attttagtct ctctcactaa aaattgtaac gctttcaaat agctattgca
    27721 ccttgttatc ggtaacagaa taaaacaaaa aaacagctga ggaaattccc tcaactgttt
    27781 tttgaaagga ttgatttatt cagggaagaa tgctgatttc acaacttctt tataaacggt
    27841 gttcactcag ctgtccctcg gtattagctc aaaaaacggc aaaatataag aaactatttt
    27901 tttcgttttt ttctctaata ctcaggccag aacactgatt tcacaacctc tttataaacg
    27961 gtgttcactc agctgtccct tggtattagc tcaaaaaaag gcaaaatatg aggaactatt
    28021 ttttcgtttt tttctctaat actcaggcca gaacactgat ttcacaacct ctttatttca
    28081 cattcaatat ccacatgaat cccatgaata caaagaacag accatacatg atgggagata
    28141 cttcttttcc tcttttagct gctaacatgg taattggata gaagataaaa cctaatgcaa
    28201 tcccatctga aatactataa gtcaaaggca tgccgatcaa gatcaagaat gaagggatcg
    28261 caatctctaa gtttccccat tcgatttgtc gcaaagattg tgccatcaat actcctacca
    28321 taatcaatgc tggtgcagtt acttgtgaag taacaactga tagtaatgga gaaaagaaca
    28381 agcctaacaa gaagaaaatc cctgtagtaa tagctgtgat acctgaacgt cctccaacgg
    28441 cgataccagc tgatgattct acaaatgcac caacaggaga agtacctaat aaggaacccg
    28501 caagcatagc tgttgaatca gaagctaaag ctttccctac acgaggcatc ttgttgtctt
    28561 tcatgaaacc tgcttggttt gctaatccaa ctaaagttcc tgctgtatca aagaaagtca
    28621 caagcaagaa tgtcagcact acgacccaca tttgaagaga attgatgtcc cctacatgtt
    28681 ttaatgcaac taaaaatgtt ggtttcaaac ttggtgctgc ggcaacaatt tcagaaggca
    28741 ttggaatcaa acctgtgacc agacctaaaa tcgttgtagc tgccatcccg atgaagatgc
    28801 cacctggtac acgccgaaca agcaagatag cagttacgac taatccaaaa atcgtcaacc
    28861 aagtagaacc aacgttgagt gatcctaatc ctactaatgt agattcattc gaaacgatga
    28921 tcccaccttc gctcagacca aggaaagcaa taaatagacc gataccgcca gaaatcgcat
    28981 atttcaaatc tgctgggatc gcatcaatga tcatttcccg cagcttgaag attgtgatca
    29041 aaataaaaat caaagaagca acgaatacgc cggctaatgc tgtttcccat ggaatcccca
    29101 ttcctaaaca aacagaataa gcaaaaaaag cattgatccc aagagcaggt gccgttccaa
    29161 ttggataacg ggctaatacc cccattaaaa tacagccgat cgcactggct aatgcagtcg
    29221 cagtaaacac tgctccttca tccattccag aagcacctaa tacagttgga tttacaaata
    29281 agatataagc cattgagata aatgttgtaa atcctgccag tacctcacgt tttaccgtgg
    29341 tgttcaattt ttctatttcg aagtaggaaa gtattttttc cttcatcttc catcccccta
    29401 aatccgaaca ttataaataa agtccgttaa aatttcgatt tattaataaa acaacgtgtt
    29461 ttatcatata ctaaaacaaa acataagtaa acaaaaaatt cggattttaa gcaaaatccg
    29521 aacgttccat ctgattaaat ccctttatct caaagttcat gaacattcac tttggattac
    29581 aagaatttct ttattgtgtt aataaagttt cttcattcta gtctcaaaaa tcaatagtgt
    29641 gatttgattt taccatttcg tttctgttct tctttgatga attcttttgt atcgacagga
    29701 ttcagattat ttagtccgcc aaaacctgta tcgaccattt ttccccagcc ttttgtcttc
    29761 ttttttcctg atttgagatt ctttgctcga agcttctcac tatgttttgc tcgccgatac
    29821 ttcaaataac cttctaatct tcccattaga tccactgttt ctcctccttt ttattcaatc
    29881 agacgactca gcttttccta taacaaaata attgccttca gggtccgaaa aattgaaagt
    29941 caagacgcca ttgttgtctg tgatttctcc tggttcttcc atgttctcgt agatagtttc
    30001 aaattcatcc gtaaaaaata ctaaagaagg cggtggtcct aatacctctg gtgaaaactt
    30061 ctgcacaaat acttttggga atacgcctaa ttctaattct tttgagatgc tgagtacaat
    30121 actcttaaat tcttctggca attcgattgt ttcagaaatt tctgcaccaa ttttttctac
    30181 aaagaatcgg caaatcatct ctacgtcatt gacataaagc atcacacgtg ttttttgtat
    30241 catcgtttga cccttctttc tatttgattt tctttcctgt ctataaactg attccattat
    30301 atcacgaaag cgcaaacacc ttgtgtttga tactattcaa gaaaaataaa gcaatagttt
    30361 ctaggattgc tttcctccac aaactttttt tacaaaaaaa aaacggcaat gaatgccgct
    30421 cttcttattt cgattctttt ataattctgc ttctgattca tctttggtta gtgtgtgtct
    30481 aacactagtt acgccaaatg tttgatcgat tttttccttc aacgcttcga ttgcctgtgc
    30541 gccacgtgcc gcttgttcag cattgatgga ttcaatgacc agcaccgctg cttgtgtttc
    30601 ttccgtcagc atcgttcttt gggcttcacg ccagttgaga cagcgacaga tcgctccttg
    30661 ttcatcatag tagatgattt cttccggaag tgccggagca tcggaatctg cacctaaagg
    30721 caggaatgat tcgtcacctt gggcttttcc taaatgcaga tttcctgcga tcttttcgat
    30781 atcttctccg ccgcaaggta cgccaaaaga caaggaaata ctattataga tatctacaag
    30841 cggattgatt gggaaaaatt cccgttcttg atttgcacgt ttcagtaaag cttcaataga
    30901 agaacgtgca ccttttttag tcttgaatgt ggtaaatgcc tgacgccact gagcaatgac
    30961 ttcgttttgg ctgaatgttt cttctgtaag gtaatttctt gcttcttttg ctgcctcatt
    31021 caataattga tgatacgttt cttcactttc ttttgttacg tggttatcga taccatttac
    31081 ggataagatt tcgatggttg cttcaggaaa tacttcccag aaattggtat ccactattac
    31141 ttttttcatc tgttcacacc tcttgattag aataattgct tgatgaatag atagtctgtt
    31201 tggggatcat ctcccatata aaaaacatgt tcgctgtatt tgataaatcc ttttttacga
    31261 taaaaagcga gcgcaacatc gttgtgttcc caaacaccga gccaaatggc tgttttttgc
    31321 ttttcttttg ctttttcaat agctgtatct agtaaaaatg taccgatacc ttgatgttta
    31381 aatttttttc gtacatagat tcgctcgatt tctaaaaaat gatcagccat ttcctcagat
    31441 tgactcgttc ctgtatttag tttcaggtaa cccgcaggct cttgatcgat actcactagg
    31501 aaaaaatctg tttctttatc ttttagctct tctgtaagtt tttctagtgc ataagcttgt
    31561 gtcaagtaat cagctaaatg ctccggtaat tatcttttcc aaatgtatct gtaaatgttt
    31621 cgatactaat ttgttgtagt aagtctacat ctgaaggcgt catttttcga atttcgtatt
    31681 tattcacggc ttcttccttt caatagatcc ttttattgcc ttttttgaca aaattccaat
    31741 cttcgctaac attcttgcat actttattta aaagatattc caattgtttt gcttcttcgt
    31801 cagaaagacc atttaatgcg actgcattgg agtagttgtt ttctcgaatg atgaaaggat
    31861 agatttctgc tcctttttta gtcggataga gtttcttgat ttttttattt tctttatctt
    31921 ccaaacgttc gatcatccca ttggattcta atttcttgat tgctcgggca gtcgttgtac
    31981 gatctacttt gatcatttct gctagttttt cttggatgat tccaggattt tcgcaaatac
    32041 gtactaaata aagatactgt cctcttgtta aatcgacttc tttgaattcg atattgctaa
    32101 tagagtctag cgctcgggca atcattccga tgtctcgaag gatttcagcc atttcatccc
    32161 acctttattt tgtttgtaag actagtttac ctctttttgt tgcaaatgca actattttta
    32221 ataaaaaaat caagttcttt tttaataact agtaaagtat attcttttct tcttttacga
    32281 aaaagctcaa tcacaataga tctggttttt gttttctaaa ataagtaact gtttcttcgt
    32341 tgttcgcttt tctgatagct ttggcaactc ttctatacta aaaaatccca tgttggaagt
    32401 ttcattattc tcaataaact tggcctcttc accgtggatc gcacaagcaa aaacaagttt
    32461 ataatactga aaaagctgtg gaatatcttt ttgtttattc gtatcaaaaa ctgctcgcaa
    32521 ctgagtagtt tctactgtta atcctgtttc ttcatagact tctttacgca cattttcttc
    32581 aggagacaat cctatttcag caaatccacc aggaagtgac cattctttcg tccgtttatc
    32641 ttcgattagt aggaccttgc catctttttt gatataggca cgaacatcta cttttggtgt
    32701 tggatgtcct tctccaatcg ttgtgagcgc ttttatttct tcgaaagttt cttttcctat
    32761 ttcactgatc agttccaatg aaatcgtccg gatctcctca aaacgctctt tatcaaatac
    32821 atcttttcca taatatagac cagcttgaga aagtgcgagt agtcttttgt attgagccaa
    32881 cttcttgttc attctcttcc cttctttttt agcaaatgta tgactatgta atcaagtata
    32941 gaagactggt ttaattacgg caaataaata taatgaaatc tgcaataaat aaaagaatgt
    33001 taaaaaactc gagaacaaaa gacattcgac ttctctgggg ctgtgaataa atggcgttca
    33061 aaagcttgca tctgcggtaa taagccccat caaacgaaaa atatgacgag ctattcttgt
    33121 ctggctgttc ttattaattg aggcaggaca gctttttcac aacctcccca ctaaacggtg
    33181 ttctcctacc aactcctcgg tcacaagtca caatattcat caaacatgag aagctgtttc
    33241 tttgaaattg ctccttgtga ctcggagctg aacgacaggc tcacgacctc tcggtaaacg
    33301 gtgttcaaaa gccaattttt cgatattaag ccatttcttt gttacttatt gtcattgatt
    33361 tctttttcta atttatccaa tagtggagtg taatccactt gagtatagtc tgttacatca
    33421 aattcatata gtgttcctaa ctggaatttg tcatattttc ggtcggcgat gatctttttg
    33481 aatgcttctt tcgtaatatg attggtccct ttcgttcgat cttccaatac gtcaccatag
    33541 tgataatgat ccatgatata gctcaaatga cgatctgatt cacgatcacg tttgtaccga
    33601 cgttgccata acacttcaaa gtctgctgtt aggcgaatcg tgatcacctg atatccgtat
    33661 ctatctgcta attcttggag tcgtgctttt tgcttatcac taaaaggata ctcggacaca
    33721 atcactttct ttcctgcttc catatacaaa tcaagtgctt tgtaatagaa aggataaact
    33781 ttcgtccttt cttgttccgc ccgttcttcc aaattgttaa acccgatgga ttctgcatag
    33841 tattccttga tttcatcgag cgtgatggca tacatatctg gaaatcgttc ccgtagttta
    33901 ttcataagat atgtttttcc tgtaccagga ctaccagcca gaagaatgaa atactttttc
    33961 atctcattta tttcctaagt taattggact ttccaagttc ataaacagtc caatcttatg
    34021 cttgacaatt tcttttactg cttcatttgc tggaatcaca ttatggcgaa gagacttgtt
    34081 gatttctgtt tcactaaagg tttctttagc tactttcgtc cagttgacta aaagctccgt
    34141 tccaacattg aatttgcgga tcccgttatt gatcaattca ggataatctt cttctttcac
    34201 acctgttcct ccatggataa ctagggggac atcaactact gcatcgattt ctttcagcaa
    34261 gggaatgttt acttctgttt ttgatttgaa ttgtccatga ttcgtaccga tagcaatagc
    34321 taatgcatcc acgcctgtag cttctacaaa tttcactgcg tcttccggtc ttgtatatac
    34381 cttgtcattt tcagcgacat ggattccttc ttctgtaccg ccgattgttc caagttctcc
    34441 ttctacggaa acaccgtgcg catgggcata ttctactact gcttttgtct tcaggatatt
    34501 ttctttgaaa ggcagatggg acccatcata catgacagaa gtatatccgc tgtcgattgc
    34561 ttctttgata tcatcaaagt ctcgggcgtg gtctaagtgc aagactgcat caaccatttc
    34621 ttcttctgca atagctgtca ttacattgac taacacatgc atgccgatat atttagcggt
    34681 atcgacactt gtttgaataa tgatcggcga acccatttct tttgctgcgc ctagcatagc
    34741 aggaagcatt tctaggttat gcgtattaaa agctccaact gtaaaattca actcttctgc
    34801 tgtttttgtt acttctttca atgttgtata catcttttat tgccacctta tctttttatt
    34861 tgtttccttt gatcgttact tgttttgcaa tttgtcgcat ctcatccatt ttgcccatgt
    34921 agtaatcgat cccttgatca tcaccagcac gagcagcatc tgcacgcttc tggttataga
    34981 ggctcatcag ttttttatac ccatctccga tcgtctgttt attttccaaa ctcttttcta
    35041 aatattggat ggcttgatcg ttcacataca attcagaaaa tttaagtcca acttgttcat
    35101 aaagtttcgc taatttatgc ttatcttggg tagtggaaat ctcttgtttc aattgtgtaa
    35161 tctctgattg aatcttttcg gtcgcttctg gcgatagggc tttttcttct tttgccagaa
    35221 cttttttcgg ttctttttct tttttcttaa aaaatccaaa catcattttc cctactttca
    35281 attaaaatgc tttcaaaact gggcttaaga tccaggcata taagagtgct gcggctactg
    35341 tatctacatc ggttgctgta gcattgatga atcccatcga attaaagatc gttactagca
    35401 aagcgggcaa taatgtaata aagaaaccat gagcaatacc gccaatgatc gctcctcggc
    35461 gtccgccaac tgcgttgcca aatatccctg ctgtccctcc ggcaaagaaa ttggttagca
    35521 taccaggtaa aatcatcgct aagccgaaca tgggcaaagt aaacatggca ataatcgtac
    35581 caatcgttgt cgtaatgaaa cctaaaatga cagcatttgg gctataagga aagaaaactg
    35641 gacaatctaa ggcagggatc gcatcaggaa ctaatttcat agctatccct cgaaatgccg
    35701 gcacgatttc acctaacagt agacgtacac cagctagcaa tacatacaca cccactacga
    35761 attggatggc ttgtaaaaag gcaaacatga tatagttttg atctcctgat aattcagaag
    35821 cgaatggttc gcctgcaaat aaaacagtga tgatatatag cgggaccatc acgaccatga
    35881 cagacaaata agtatcttgc agaaattcga aatgtgtcgg caacttgata tcttcgatcg
    35941 actttttatt ttcgcctttc tcaccaaaaa gcttagcaac accggcttca aataagtacc
    36001 cgatcgtaca aaaatgacct aaagcaatat catttgatcc agtgatttta cgaatgatcg
    36061 gttgcgctac tgccggcata gcaatggcga aacaagctcc gataaaacca ccgaccaaga
    36121 tcaatacgat ccctcgcaga ccggcaaaat agccaaagac tgtcgtcatc gttgccatcc
    36181 ataaaattgc ttgaccagtt aaaaaaatgt acttccattt tgtgaaacga gccaaaataa
    36241 tattgaatac aaaaatggct aagaacgtca gtgcaatatc tctacctaaa cccaaatcat
    36301 tcattgcttg accattgata gattcaatag aagggatgat cccttgcata tgaaatcctt
    36361 cagtaaagat tttcccgaaa taagttaaac ttccaacgat gatgctcgaa ccggcactca
    36421 acacttgaaa tcctaggagt gtctttaacg tgccagagat gacttgtcct gctgatttct
    36481 tctgcagtag tagacccaac atagcaatca atgcaatggt gatcgatgcc tgagtcaaaa
    36541 tattttcgat gataaaattg ataacgccca tgtttttacc ctcttttctt tttgtattta
    36601 gataactccc ttatcttgca gaactggggt gattttttct tcaatttcat tttttgaaac
    36661 aatatttttc aaatagatca tcgccgtttt aggatcaact gcgaactttt cgaactgcga
    36721 acgaaaattt tctgctgtga tgatcacatc tggtttcatg gaaactgcgg aagaaatatc
    36781 acaatggtcc agtttggctt ctactcctaa tttgttcaaa acatcctcag cagacatctg
    36841 tgctgcaaaa ctacttccta aacctgcgcc gcatacgaat aaaatgttca atttgttcat
    36901 ctttgttctc ccccgtatca tttattattt ctcaactact gaaaaaagca gttgtttgaa
    36961 atcttcgata tttgtgcatt caatcaatcg atccaacttc tcttcgttgt tgatcaattc
    37021 aattagttct ttcatgatgt tcagatggga gtaggagtct actgctgcca aacaaaaaat
    37081 aatctttact ggatccattt caggattacc gaaatcgatt ggttgctcaa tcgtagctac
    37141 gctgactcct aatttattga ccccatcttc tggtcgggca tgggctagtg ccatgtgttt
    37201 gccaatcacg atatacggtc cgtaatcatt cactgcacga atcatcgctt cgacatagcg
    37261 ctcttcgaca atgttttctt taaccaatgg tctagcagct agttcaatcg attcctccca
    37321 agtagtggct ttttctttga tcatgatatt gctgtccgtc aaaatatctt ttaacatagg
    37381 ctggatctcc cttttgttga tttctaaatt gtttgtttcg aataattttt ctaacttgcc
    37441 atagatgatt cgatctactc ggccaccact ttctttcatg acatctatta tttgagtgaa
    37501 taactccgtc gattcatgac tgttaggctc taaccgctgt agttcttcat ttcttttcaa
    37561 aaagtcttct ataaccaaga tattcgtctc agttaaaatc ggatcgacaa ctaaaagcgg
    37621 cttatttcga taattcaatt gaaaggtgct gaaaaccaga ttgacatcca atttatctac
    37681 taattcgatt tcttttgaac ttaaaactgc agtaatctcg atctgaggaa acttttcttt
    37741 cagattctct gctaacagat ttccagtagc catcccatgg ttacagacaa cgactgattt
    37801 ataaacataa ttcaagtctc gcttgattac actggcgatc gtcgaaaagt ggatgacaag
    37861 aaaagctatc tcgtcctcca gtatcttgcc accaattact tcttcgatcg taggaacaaa
    37921 atgagtgact gcgcggtaga ttgggaaata atttgttcgg atgttttctt tcaatggatt
    37981 catgacttgg atatgattct tgattcttac aatcagtgca gccatgtgct tatagagatt
    38041 ttcgaataag gcttcttcct ttagatggaa aggtacgtgt gtctcgttct ctacaaattg
    38101 gatcaattgg atcgttagta attgagcttg tacccactcg attgaattgg agatatctct
    38161 agcattgaac gtctcaatag tgaaacgaag ataatttctt tctacttgcg ggacattgtc
    38221 caattgaaat tcctcgatca tgtggtcaat aaatgcagaa aagctactgt cttttatttc
    38281 tgtatttttc caattaatgg cagagataaa ttcatgtttc ctgattcgct ggatccagat
    38341 caatgtaaaa agcaggattt gttttttata aatatcatct tcttgtttca acaacttatt
    38401 ggaatagatc tcctgtattt ttttgatttc ctccaaagga aaataatgga gaaagatttt
    38461 ttgagccggt gttaattgtg catcttgatt cataaagaag tcggtcattc ccagattctg
    38521 attgataaaa tcatagatca tcgtacggat agatcgttct gaacctttat aaaccagtcc
    38581 ttgctttcca taactgacga tctctatccc ataactgacc agtgttgctc gtaaccgtct
    38641 catatcttca tctaatgtac ttttagagat ttgaaattct tcttcttttt tgtttagatg
    38701 gatcgggatt cggctgtaag caacggctaa aactagatca aaaatacgtt cgcgccgatt
    38761 caaaaagccg tctttcacat cttttatttc agtttctaat cgagcttgct tttccttttc
    38821 cagcttcaac aaaaaacctt tgttgcgaat tgtacggatc tccggaagct ttttactgct
    38881 caaccagata ttcgcttcaa gaacttcctg acgaatcgtc cgctgactga cattgaactc
    38941 acttgctaga tgttttgctt ttactggata ttgggctgtc atgattctgt gaagtatttc
    39001 aattgttctt gttttcatag aaatgatctc cttctgattt caagtatatg gttccgctta
    39061 cataataaaa agtagatatt tctgccactt gaagtggaaa gaaagtaagc aaagcaaaca
    39121 aaaaataagc catatgcttt ttcatctcct caacgaaaaa gacatacgcc tattattaca
    39181 aaaattttgc ccatctatac aaaaaagagg ttgtgaaaaa gctgtcctgc atcaagtaat
    39241 aagaacaact aaactaaaat agtgcttcct attttttgtt tggttgttct tattatcgca
    39301 gatgcaagct tttaaacacc atttagtgga gaagctgtga cagagatgtt cagccttgag
    39361 cgataagaag cgattttgaa aaatcgcttc ccatatttta caaaatcgtg ccttattgcc
    39421 gataggctat ctctggaaca ccgtttattc agaaaaagga gctgcgacaa ggcttttgtc
    39481 acaactccgt tcataaatat ttcctgtcaa tccagcataa tcggttgaat aaaatcggct
    39541 tgccatcttt tttctttctc agaaaaatca aaataccata tctgagaaaa atactgtcgt
    39601 tgtgtcttac ttcctgagat caatcgccca tcttttcgat cgactgaata atcacagcat
    39661 ctgaaatcaa ttcggatggc aaaactattt tcgtggatct gtcgataatt tcctacttcc
    39721 tgaatagtta tttttttagt atgatttctt acaccgtctg ctttgttttt ttgcagaact
    39781 ttctgatgtt cggcaaacag cttgtctgta taacagcttc tggctttttc aggagtttct
    39841 tcttcccaag cggattggat cttcatgaat atcttttgga tttccaaaaa ctgtgctttc
    39901 ttttctttag ccgttcctga catgcgggac aatatctccg atttcagcct tttcttttgt
    39961 cttttattct tgacgataga aaaagcagaa acccctccta ctgacaaaac agctaatgca
    40021 agaaaccccg tttgctcata actagaagga cctgtacttg gaccataata cccataagaa
    40081 tctctgccgt aagaacgact gccaaaataa ctgcccccat atgaacgacc gccaaatgaa
    40141 cctctgcccg aacttcgacc gctagatgta cctccaccat gaccacccgc gacagcgaat
    40201 ccttgggtcg gcagagcaaa caaacatagg cccactaaca aaatgattgc taaataattg
    40261 attttttcat tgtcttcctc gttatctttt attacaaaaa actattttta tcatagcctt
    40321 gctaaaagga agagtcaata accagatgca taaactgttt ttttagcaat tttgtcttat
    40381 ttagtcattg agtaacatcg gacagatgta tgtgtcggca gtccataatt cctctatctt
    40441 cagcatgaac aagcttgcag acaagagatc tgcgcttcct ccaggagaga tttggttttc
    40501 ctgacaaaag tgcaacagtg ctgtaaatgg acgatttggt aaagcacttt gactagcagc
    40561 atgagcaagt ttttgcattt ccactaacgt tttctgatca ctgcgatgca atacgcatgt
    40621 gtcatctagc gaactcatca aaactaacaa catatgcatt tgtttctgta catatgaatc
    40681 attatctagc tccttagctg cttggattgc aagcctgata tgcggatacc ctaatatcgc
    40741 ttcttcatat gcaccattta cgccatattt ttgcttcacc ctatgaccat gagtggtttt
    40801 tttatgacga tattttgtat caggaaaagc agcaagcttg ctgacatcat caagtatttt
    40861 cgataaagaa aaaggctgtt ccatactgat tttttgtgca atgacactga tcaatagccc
    40921 cattgcccaa atcgctcctt tatgggtatt gatgccattt gtagcaatca gcattgcctt
    40981 ttccgctttt cgcccaatct cagcaattct ttctcttaac tcctgatcaa tagcataatg
    41041 ccatgctatt tgagccattt cttcaaaaaa aggagttagc gtcttagcag acttatccaa
    41101 aatgcgccga ccttctttta cggcttttgc gatcgtatgg aaattatcat ctcctaaaac
    41161 catatcagct gcctgtttgc ttacttcact acctttgatt cccatagcga ttcctatatc
    41221 ggctcttttc aaagcaggtg catcatttac gccgtctccg gtcattccga cgatttctcc
    41281 atttttctgc aaagctttca cgattcgcag tttgtgttct ggtgtcgttc gagcaaagac
    41341 gtcgactttt ggtacttgtc gaatcaactc ttcttcagac ataagatcca aatcgatccc
    41401 ttctaaaact ttagcagtat gttttaagcc aatttgttcc gcgatcgctt ttgctgtatc
    41461 tttgtgatcc cccgtaatca ttttcactga gattcctgct tgttggcatt cctttacagc
    41521 tttgactgca ctttcttttg gcggatcaat caacccagca atccctgcaa aagttaattg
    41581 ggttaaagac tcatgcgtga gttcctgttt cgaatctact tttttgtaag caaatcctaa
    41641 aacacgctga ccttttttcg caaacgctgt ggctgtttga ccccatgctt cttattctga
    41701 agaagaaaga gttgatttag ccaataatac ttctggcgca cctttgacgt aaatcacccc
    41761 tcctttttcc tccgcatgca tcgttgccat atatttgtat tcagagctga acggaatttt
    41821 tgcttctatt tttttcaaag gcagatcggc tgtttcggtg tggcgaatca gtgcaagttc
    41881 tgtcggattg ccacttaaat tttctgtttt ctgttcttct ttcgtcttta attcctgaca
    41941 atttttcata ataaacagta cgcgagtttc ttcctttgct gctacatcca caactgtcat
    42001 ttcatttttt gtcaatgtcc ctgtcttatc tgaacagatc actgtcatag aacctaatgt
    42061 ttctacagaa ggcagtcctt tgatgatcgc cttttctttt gccatttcat gtactccgac
    42121 agataggatc atggtcaaca cagctggcaa tccttcgggt accattgaca ccaccaaagc
    42181 gatcatagct gaaagaagga tatgccaatc catgccatag cggaatgttg taaaaacaac
    42241 taagaacaag atcaaacaaa gaatgccttg aaaaatttgt ttgttcaatc ggttgatctt
    42301 cttgatcaga ggcgtcgttt gttgttcgac cgattgcagt gcttgattga ttttaccgat
    42361 ttctgtcgtg tttcctgttt ctacaacgac accgactgca gaaccagatt gtacaagcgt
    42421 tcctgaaaaa gccatatttt tttggtcacc gattgattct tctgctgcca aaacggaatg
    42481 atttttttca gatgcttctg attctcccgt caaaatcgat tcttctacta gtagatcatg
    42541 tacttcgatc aaccgaacat ctgctggaac gacgtctccg gcacgtaaga caatgacatc
    42601 tccttttact agttctttcg cagggatttt ttcttgcatc ccgttcgata gtactatcgc
    42661 ttcttgtccc atcagctgct tcaaaccatt caacgattct tctgcttttc tttcttgcca
    42721 ataactaacg agtccgttga cgatcactac gaataaaata atacttcctt cgatgtattc
    42781 gctcgaagca aatttcaaaa aagaggcaat gatcaacacg atcatcagca gatcagtaaa
    42841 gtgttttgct atttttttcc atcgttttgt ctgctgtctt gcttctatct cattatatcc
    42901 ttgatcagtc aggcgtttct gccgttcttc ttgcgacaag ccttctgttg atgtttgata
    42961 gtttttcaat acatcttcta ccgtcaatct gtaccagttc atcctatccc ctcctgttta
    43021 attgatactg ttattatacg cagggaagtt ttttcaggcg tatcgattat ttcagggggg
    43081 tctgaaataa ttcgttttgc agaatgtatc tgggcctaac tgatatttct attgcttatt
    43141 cttttatata caaaatttaa gaatacaaac cagataacca ataaaaaagg aataaagcgt
    43201 gtcgtgcttt cgaataacaa gctgaaagca attgaagtaa aaaacaccaa caaagcaaga
    43261 tctttatatc tttgtgtagt gttccgtttc ttttctttaa taaattgaac atgtgcacac
    43321 aagatcgaag accacacaaa tagaaaacaa ctcgttgtca tgcctgccaa aagctgaaaa
    43381 aacaatcgac tatcagctac aaaaaaatgt agacaaagac cgacaaaaaa taacatccct
    43441 gtaattaaaa taccgtgtaa aggtacgcct ttcttagaaa gcgcacaaaa ccaatggggc
    43501 aaacttttct cttttgctgt ttgatataaa atcctgctag tactgtagac tgcactattt
    43561 gctgaggaca aagaagcaac caaaatcatt agattgacaa ttgctgctcc gctttggcaa
    43621 tggatcactt gcagcatagt cactaaagga ctttctttta aatctaaaca atgccatgga
    43681 acaacaaata aaatcgacaa aatcgttcct atatagaaaa agaggatacg ccaaggcaga
    43741 cagttgattg catttttcaa attcttttgt gggtcactcg tctcaccaac tgttaatccg
    43801 atcatttcaa ttccagcaaa actgaataaa accataggaa atgaagcaat cattccagca
    43861 aagccaaacg ggactaattc attgagctgt ccaaaatttg ctttgattcc aatagactgg
    43921 gtatgaaaat ctgagacact caatcctata cccaataaga taaaaaggaa aatccccaag
    43981 atcttgatga ttgagaaaaa agattccaat tcaccaaacc acttgactgc aacaaggttg
    44041 actgccatta agatcattag tgcgctacct ccgcttatca aagctggtac atgtacaaac
    44101 cagaaatgga tatacaatcc tattgctgtt aattccgcaa gtccagctcc aagccagcaa
    44161 agccaataag tccaattgac aaaaaatgcc cactttttgc ctaaataata gtcaacgaaa
    44221 tcactgatag agttaaatgt actatttgct aaaaataaat cacccatgac ccgcatcaaa
    44281 ccataaatag ccaaaccgca tatcaaataa acgagaataa cagctggccc tgctgatgca
    44341 attgcctttg ctgaacctaa aaataatcct gttccaattg cgccacctaa tgcgattaac
    44401 tggatatttc gattagttag agtccgtttc attgccggtt tttccatcaa gttcaccttt
    44461 ttctttttca agcagcatgg cgacttcctg ttcccaagaa ggtttcttta ttttttcaat
    44521 acagagtggt actacaaaca aaatgacact gatggctgtg actgcaatta gattgatcca
    44581 tgtcccagct ttcatcgtca gtccgacaga aaatccgatt gccgcaaata aaacaataac
    44641 gacagtccaa actccaagat tgctcttttt ctcttttttt ccaattctaa atggtcgatt
    44701 caactttgca tgtgtataac gcaatctcaa aactgcgatc cccatgatga catagacgat
    44761 acagtagata acagtagttg aatttacgag catcaaaaat gcttgattga cgccaggaat
    44821 cagcaaataa aaaagtgcaa agattgagat aatgacagac tgagtcaaaa taacggattt
    44881 ggatacgtca aattgattcg tcttccaaaa attgaatttt ggtggatacg caccttttct
    44941 agcactttct gtcactgtct ttgctggtcc tgaggcccaa gcagataatt ggactgctac
    45001 accaatgaag acaagtaaac taaacagatt cacaataaga tgtggcaaac caagaatttg
    45061 gcaataaatc gaaatagatt gcgcgatatt attcaattcc atttgtcctt ttggtacgac
    45121 atttgctaca actaaagcat tcaaaatatt gacgaaaaat ataaaaatta acgcggcaaa
    45181 tactccgcga atatacgttt taactggatt tttcaaccga gtgatataca cagaggacat
    45241 ttcgatacca gtaaaaataa acatgatcgg cgcaaaataa acaaatgagg atgcagtcgt
    45301 agtatctggt attaatttgt cccatgaaaa actgcctaat gttccggttg gtacgatccc
    45361 aactttgatc cacgcagcaa accctaataa gagcatcatc gtcaaaggca cgtacaatcc
    45421 taaccaaaca ccgacttttc cgccgatttt tgccatatca aatttcaaat tcaaaaaagt
    45481 gatgatccaa taaacgacta aaatgacaac taatgtaaat ttgctgttca aaccaagcgg
    45541 cacattatcg ataatattac caaacaacgg agcaagtgct gaagcgacca tcaccatccc
    45601 aggaaacatt tggacccata acagccatga gacaacgaac ccccattttt ttcctagagc
    45661 attcgatacc caaagttctg gaccaccttt ttggggaaac attccggcaa attcaccaga
    45721 gatcaatgag attggaaaag cgtacaataa tactgcaacc agcatgtaaa aaatcatcgt
    45781 ccagctcgtt gctgctacat ctggaatatt tcgtacactt gctacaaggg cgcaggtcat
    45841 tccgataaag gtcatggtcg tgatttgctt tgatgaacca gtatctttca tctttattcc
    45901 ttctttcaat agcgacatat gtttttgttt tcttttattt ttgttcaaga acagaaatga
    45961 caaggtttca tcgattcagt acaaaaccca gtatcaccga aacatccatt gaacgatctt
    46021 agtgaactgg gtttgataca tgaaacgaac gatccaactt atagaacgtt ttataaaaaa
    46081 gaatatcttg gcgaggaata tatgaaaaac agggggaaat acaattgttt tgatgaagca
    46141 aataattgtc tgttctccat atgtctcttg ttattctttc acacaaaaat cagttctatt
    46201 ggatgatttc gtttagtttc ttttccattg ctttggcgat ttcttttgta taaaaattga
    46261 aaattcgatc atcattcaaa taaggagtca tgatagccgc acgtagcaga gtgattttct
    46321 gttctttttt ccattcttct ttcgttagac ccattctttc aacaaaacga atcggagaat
    46381 caccgtaatc ttcttgtgta aatgtagtat gagaagtaat aaaccgttca ccataaacat
    46441 cgccatccat ataagaagac cgatcgaaca ttttttcatt taattcattg atagcgttca
    46501 aatctgtaca gccttgttct ttgaataccc aattgaccat attaaaatca ggatgattca
    46561 atggatagac ttcgattgtt tttcctttaa cagtaaaagt cagatgatcg agaaattctc
    46621 tgaatctttg agctgcctcg attgaagccc caatcaattg accatatccg gtcacattca
    46681 atggcaatac acgatgtgct gtccagactg cggcggcggt tgctccagct tttgatcctt
    46741 ccaaaatata agctcctaac atatctggag cctttactga tttttcaaac acatatggcg
    46801 cgaaataaga aatgatattt ctcatatttt tatgtttgat tacgattcct ccagcagcat
    46861 aagggacata gcccatctta tgaggatcta tcgtgacaga atccgcttct gaaatagctc
    46921 tgaacccttc atatacctct ttgtcaatcg ttacgcagtg gtggaaaaca tgatgttctt
    46981 caaagaattc agctaaggaa gcatacggca caaattctcc tgcttcgttt agaaacagtg
    47041 aacgcgcata gccgccatat gcggcatcaa catgtaaata gaaatagatc ccttcatctt
    47101 ttaacctttc tcttaattgg acgattttat ctacactgtc tacttgacct tcttctgtcg
    47161 ttcctacgac agcaaccaca cctagaattg ggattttttg ttcagctaat tcacgaattg
    47221 ttttttccaa agcattaata tccattcgat aatcctcttg aactgggata gcaaccatct
    47281 gatctaagcc tactccgcaa atatctaacg ccttcatcca agaatagtgt tttgtttgag
    47341 gcaccagcca tttccctaat ctttggatat tttttccgct acgggaagaa gctgctttga
    47401 cctcatccat ttcttcgtcc gtaaagcttt cggtcatttc caaaatttct tcaacagata
    47461 aattcaaaag ttcccactca gacatttttt tcacttgttc aggcagcact tccttgacag
    47521 ctaaaggaat cgatttgatg cagcgggcgt accataatcc ttcaagatta gcaatagaac
    47581 catctgctgc aatgtgtccc catccgtctg tcatattgaa taatgaagca aaatcctgtc
    47641 ctacttctgc ttccatttgc gaagttgcca tagaagattc caatgcaaca ttatttgggt
    47701 tccacagcat cgcatagttg taagctaata aagcaggcat caatgtttca gcattcattt
    47761 gtccccagta acggcctgct gaatgccatg ggatcgatcc tgcgcgtaat cgctgactga
    47821 cttcatccaa gacctctatc atatggtcgc gggtggctag ataatctgga gtgagcttat
    47881 ctccttcgct aattgcaggc atatctgaag gaatataatt ttctctccat ccaagatgtt
    47941 catctaccat cttgttcaac aacattttat aaactggtcc attttcagct ttgtcgccta
    48001 taaagacggc cttgatatcc atatctttca tttttcttcc ctccatattt tgttggtcaa
    48061 tgtctcgcag atacatgtgt ttatctgtaa cacctcttca ctatagcgcc aaaaatgaga
    48121 aatttttttt ataacatcaa atccacgtag atttttacaa cgcgaattgt aaaattaagt
    48181 tagaaaaata aaaaggcatt tatggtacac tcaaattgta tttccgacga aagaaaacaa
    48241 aggagtgtaa ccataaatga cctacaaaca tcttaccata gacgaactga caatgataga
    48301 atcatattat cttcaacata ataagccggt tgaaatcgct aaccgaatgg gacgtgctat
    48361 acaaactatt tataatgtag tcaataagtt caagcaaggc aagactgctc ttgattattg
    48421 gcaccagtat aaagaaaata agaaaaaatg tggtagaaaa gtcattcaat tacctgctca
    48481 tgaagtagat tacattaaag agaaagtcac tcttggttgg acgcctgacg tcattatcgg
    48541 gcgaaaagaa agg
//
LOCUS       DASHHB010000016        48481 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.016, whole genome shotgun sequence.
ACCESSION   DASHHB010000016 DASHHB010000000
VERSION     DASHHB010000016.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 48481)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 48481)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..48481
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.016"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(29..376)
                     /gene="rbfA"
                     /locus_tag="U2K71_000990"
     CDS             complement(29..376)
                     /gene="rbfA"
                     /locus_tag="U2K71_000990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HEM7315093.1"
                     /translation="MANYRDRRVGQEILKEVNDILRKKVRDPRVENVTITDVKVTGDL
                     QQATIYYSILSDLASDKKTAQDGLNKASGLIRRELGHNLSIYKTPELFFELDESVVYG
                     NHIDELLRDLNKD"
     gene            complement(403..2709)
                     /gene="infB"
                     /locus_tag="U2K71_000991"
     CDS             complement(403..2709)
                     /gene="infB"
                     /locus_tag="U2K71_000991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HEM7315094.1"
                     /translation="MGNKRIYELAKELKKSSKDVVEKAQQLGIDVKNHMGAITSNDEK
                     KLQQAFKNPSTKQQAAQQASRKPANQQPNDQRKKETTNNQQKNKNNRSYQDRGQGSGQ
                     VNQGKKPTTNQNNTGNTSSQNRQGTSQGGNQNRTNNNNKPNNNNRGKFNNNNRNRYNK
                     KGKKGKQQQSTKPAVPPRKFRELPDVLEYTEGMNVADIAKKIHREPAEIIKKLFMLGV
                     MVNQNQALDKDTIELLATDYGMEPQEKVQVDIADIDKFFEAEEVDPDKLVSRPPVVTI
                     MGHVDHGKTTLLDTLRHSRVTSGEAGGITQHIGAYQIDIDGKPITFLDTPGHAAFTSM
                     RARGASITDITILVVAADDGVMPQTVEAINHAKAAGVPIIVAVNKIDKPGANPQHVMQ
                     ELSEYELIPEAWGGETIFVEISAKFGQNIEELLEMILLVAEVEDLKADPTQRAIGTVI
                     EARLDKGKGPVSTLLVQEGTLHVGDPIVVGNTYGRVRVMTNDLGRRDKEAGPATPVEI
                     TGLNDVPQAGDRFVVFEDEKTARAAGEERAKRALLENRAATSRVTLDNLFESLKEGEL
                     KEVNVIIKADVQGSAEALAASLKKIDVEGVRVKIVHSAVGAINESDVTLAAASNAIII
                     GFNVRPTPQAKVQADTEEVDIRLHRIIYKAIEEIETAMKGMLDPEFEEKITGQMIVRE
                     TFKVSKVGTIAGAYVTEGYIRRDSGVRVIRDGIVIYEGQLASLKRFKDDVKEVKMGYE
                     CGAMIEKFNDIKVDDVIEGFIMEEVKND"
     gene            complement(2722..3033)
                     /locus_tag="U2K71_000992"
     CDS             complement(2722..3033)
                     /locus_tag="U2K71_000992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxQ-related RNA-binding protein"
                     /protein_id="HEM7315095.1"
                     /translation="MNTVNRQKAMNLIGLAMRAGKLITGEELTIAEIRKQKAKLVFVA
                     TDASENTRKKIKDKSSYYEVPCFELFSEAEITQMIGKPRKVFGIMDAGFAKKTKELIE
                     G"
     gene            complement(3030..3323)
                     /locus_tag="U2K71_000993"
     CDS             complement(3030..3323)
                     /locus_tag="U2K71_000993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlxR family protein"
                     /protein_id="HEM7315096.1"
                     /translation="MKQRKIPLRKSVVSGEMKPKKEMVRITRSKEGEVSIDPTGKMPG
                     RGAYVSLEPEEVQQAWDKKILDRVLETTLTDEFYQELLDYVTHQKARKELFGK"
     gene            complement(3345..4520)
                     /gene="nusA"
                     /locus_tag="U2K71_000994"
     CDS             complement(3345..4520)
                     /gene="nusA"
                     /locus_tag="U2K71_000994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusA"
                     /protein_id="HEM7315097.1"
                     /translation="MSKEMLNALDALEAEKGISKEIVIDALEAALVSAYKRHYGQAQN
                     VEVEFDQKKGKIHVYAVKEVTEEVMDSQLEVSLKDALLINPAYEIGDTIRFEVTPKDF
                     GRIAAQTAKQVILQRVREAERTIIYNEFSAYEKDIMQGIVERQDKRYIYVNLGKIEAV
                     LSKQDQMPNEFYQPHDRIKVYVSRVENTSKGPQVFVSRSHPDLLRRLFEQEVPEVYDG
                     IVEIVSIAREAGDRSKVAVRSNDPNIDAVGTCVGPKGQRVQAIVNELKGENMDIVEWN
                     EDPAVFIANALNPAQVMDVIFDETNPKACTVVVPDYQLSLAIGKRGQNARLAAKLTNH
                     KIDIKSESDMTEFYENQEQQKETEELHDEAIVQSDLTDDEYETIAFNNETVEEKPEA"
     gene            complement(4543..5016)
                     /gene="rimP"
                     /locus_tag="U2K71_000995"
     CDS             complement(4543..5016)
                     /gene="rimP"
                     /locus_tag="U2K71_000995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HEM7315098.1"
                     /translation="MSSVVETVTEMVTPILDEQNFELVEVEFVKEGKSWFLRVFIDKE
                     GGIDIEECAFVSEKLSEKLDAADPDPIPQAYFLEVSSPGAERPLKKESDYEKALGDYV
                     HVSLYQPVDGEKQYEGFLQSFDAEQLTLKIRIKTREKELTFDRKNIAKARLAIQF"
     gene            complement(5161..9519)
                     /locus_tag="U2K71_000996"
     CDS             complement(5161..9519)
                     /locus_tag="U2K71_000996"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PolC-type DNA polymerase III"
                     /protein_id="HEM7315099.1"
                     /translation="MSEKRDLFEKLLEQIQLEEAEKNHPLLTAGEMDSVVVHRKSRLW
                     EFTLHFSKILPIMLYRSLTQHLTIAFKDIAQVKLNILADDQTFDEQLLQDYWAQALEN
                     QQCDTPLAQQVLKTQVPVIKDRKVILPIDSTGAISYLKQQYLPLVEELFVSYGFPKFR
                     IEPEVDEQQAERVLKLFEERKQEQAEAFMKQAAESLVVHEQKKKERKEQSPALEGPIH
                     IQLGRNIPADEPTTPMISIVEEERRVTLEGYVFDKEVRELRSKRKILTLKITDYTSSF
                     IVKKFSNGEKDEQVFDAISVGSWLKVRGSIQEDTFVRDLVMNAQDIIEVKHTPRKDYA
                     PEGEKRVELHVHSNMSTMDATNSISDLVAQAGKWGHRAIAITDHGGAQAFPEAHSAGK
                     KAGVKILYGVEANVVDDGVPIAYNDAHEALSEATYVVFDVETTGLSAVYDTIIELAAV
                     KMYKGNVIESFDEFIDPGHPLSRTTVDLTGITDGMVRGSKSEEEVLRMFLEFSKDTIL
                     VAHNAAFDMGFLNTSYARYGIPEAANPVIDTLELARYLYPQFKRFGLGVLSKKFGVSL
                     EQHHRAIYDAEATGHLAWIFVKEAMDNHNMLYHDQLNEHIGEGDSYKRARPFHVTILA
                     KNQAGLKDLFKLISMSNVEYFERVPRIPRSQLKKMRENLLIGSACDKGEIFEAMMQKG
                     VEEARNRAKFYDYIEVMPKAVYAPLIEQELVKNEHDLEEIIQNLVEIGKSLDKIVVAT
                     GNVHYLNEEDAIYRKILINSMGGANPLNRHSLPDVHFRTTDEMLTAFHFLGEETAKEI
                     VVENTNKIADICEEVIPVKDELYTPKIPGSEDEISELSYTKAKQMYGDPLPEIIQKRL
                     KKELNSINGNGFSVIYLIAQKLVHKSNEDGYLVGSRGSVGSSFVATMTGITEVNPLAP
                     HYYCPECQYSEFFEDGTYGSGFDMPEKQCPKCGARLNKDGHDIPFETFLGFHGDKVPD
                     IDLNFSGDYQAEAHNYTKVLFGEDYVYRAGTIGTVADKTAYGYVKGYERDNNLQFRSA
                     EVDRLAKGATGVKRTTGQHPGGIIVIPDYMDVYDFTPIQYPADDQNSEWKTTHFDFHS
                     IHDNVLKLDILGHDDPTVIRMLQDLSGIDPQTIPTDDPEVMRIFAGPEVLGVSQEQIY
                     SKTGTLGIPEFGTRFVRGMLEETHPTTFAELLQISGLSHGTDVWLGNAEELIRRGDAT
                     LAEVIGCRDDIMVYLIHAGLDSGMAFKIMETVRKGQWNKIPDELRETYLSAMKENNVP
                     DWYIDSCSKIKYMFPKAHAAAYVLMALRVAYFKVYFPILYYCAYFSVRADDFDLVSMC
                     KGKDAVKQAMKEITDKGLDASVKEKNQLTVLELANEMLERGFKFGMIDLYKSDAVNFV
                     IEGDTLIAPFRAVPSLGTNVAKQIVEARKDGPFLSKEDLATRGKVSKTLIEYMNDNGV
                     LKDLPDENQLSLFDML"
     gene            complement(9731..11440)
                     /locus_tag="U2K71_000997"
     CDS             complement(9731..11440)
                     /locus_tag="U2K71_000997"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HEM7315100.1"
                     /translation="MRQSKMLIPTLREVPNDAEVLSHQILLRAGYIRQVSAGIYSYLP
                     LANRVLEKLKRIMREEFEKIGAVEMLMPAILPAELWEESGRYETYGPNLYRFKDRNDR
                     KMILGPTHEETFTDLMRNEINSYKKLPLNLYQIQPKYRDEKRPRFGLLRGREFIMKDA
                     YSFHATEESLDETYKDYEKAYTEVFKRCGLEFRSIIGDGGAMGGKDSKEFMAISEIGE
                     DTICYSTESDYAANLEMATSYYVSKKSHETQLELEKVATPNVKSIDEVADFFSVEPQK
                     IIKSVLFIADEQPVLVLVRGDHEVNDVKLKNFLSADFLEEATEEEAQKYLGAEFGSVG
                     PVGVSDDVKVYADRYVQDLANAITGANETGFHYKNVNPERDFTVLSYEDLRFVQEGDP
                     SPDNNGVLAFTRGIEIGHIFKIGTRYSESMGATVLDENGREKFVIMGCYGIGVSRLLS
                     AIVEQHSDEQGINWPKGIAPFDLHIVQMNLKDEYQTNLTNELEEAMTKAGYEVLVDDR
                     NERAGVKFADSDLIGCPIRITVGKKAVDGIVEVKIKKTGEMVEVRKEELVNTLPILLN
                     QGE"
     gene            complement(11508..12776)
                     /gene="rseP"
                     /locus_tag="U2K71_000998"
     CDS             complement(11508..12776)
                     /gene="rseP"
                     /locus_tag="U2K71_000998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIP metalloprotease RseP"
                     /protein_id="HEM7315101.1"
                     /translation="MKTILTFIIVFGILVIVHEFGHFFFAKRSGILVREFAIGMGPKI
                     YGHQAKDGTTYTLRLLPIGGYVRMAGNGDDETEMAPGMPLSLLLNSDGIVEKINLSKK
                     IQLTNAIPMELSRYDLEDELTITGYVNGDETEVVTYPVDHDATIIENDGTEIRIAPKD
                     VQFQSAKLWQRMLTNFAGPMNNFILAILLFIILAFMQGGVQVTNTNRVGEIMPNGAAA
                     EAGLKENDEVVSVDGKEIHSWNDLTTVITKNPDKTLDFKIEREGQVQSVDVTPKSVES
                     NGEKVGQLGIKAPMNTGFMDKIIGGTRQAFSGSLEIFKALGSLFTGFSLDKLGGPVMM
                     YQLSSEAANQGVTTVISLMALLSMNLGIVNLLPIPALDGGKLVLNIFEGIRGKPLSQE
                     KEGILTLAGFGFLMLLMVLVTWNDIQRFFF"
     gene            complement(12937..13737)
                     /locus_tag="U2K71_000999"
     CDS             complement(12937..13737)
                     /locus_tag="U2K71_000999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HEM7315102.1"
                     /translation="MKQRVVTAAVALVLFIPIIWIGGIAVELTAAILAVIGVYELFRM
                     KGLTLLSFEGVLSAVGAVLLVLPKERWFFFLPENVSTFMLFYLIVMILLGTSVISKNT
                     YTIDEAGFPVLVSLYVGIGFQNFVNARTESLAVLIFGLFIVWATDIGAYMVGRRYGQH
                     KLSPDISPNKTIEGALGGIGSAVVVAFLYLLLYPAKELFNHGMFVMLLMTIIFSAVGQ
                     FGDLVESAIKRHYDVKDSGNILPGHGGILDRFDSLLFVFPIMHLFGII"
     gene            complement(13734..14546)
                     /locus_tag="U2K71_001000"
     CDS             complement(13734..14546)
                     /locus_tag="U2K71_001000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HEM7315103.1"
                     /translation="MLRFFPQKNKYIQEKSEFQFNTDGPIPQHIAIIMDGNGRWAQNR
                     RLPRVAGHKEGMETVKKVTKKASRLGVKVLTLYAFSTENWKRPKDEVSFLMQLPVDFF
                     DTFVPELIKENVKVHVMGYENVLPEHTQDAVRRAIEQTKNNTGMVLNFALNYGSRAEI
                     VTAVQEIAEEVAKGEIHAEEIDDELIAKHLMTGFLPKELQDPELMIRTSGEERISNFL
                     LWQIAYSELYFTKALWPDFDGAHLEEAIASYQNRDRRFGGVKKTEDEKGDQS"
     gene            complement(14741..15298)
                     /gene="frr"
                     /locus_tag="U2K71_001001"
     CDS             complement(14741..15298)
                     /gene="frr"
                     /locus_tag="U2K71_001001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HEM7315104.1"
                     /translation="MADAIMTEAKEKMKKAAQNLQRELGQIRAGRANASLLDRITVNY
                     YGAPTPLNQMASIQIPEARVLMITPFDKSILQDVEKAIMASDIGISPTNDGNVIRLVI
                     PQLTEERRKELAKDVKKEAENAKIAIRNIRRDAIDEYKKQQKNGDITEDDLRGLEKDV
                     QTLTDNSIKEVDKIAADKEKELLEV"
     gene            complement(15301..16023)
                     /locus_tag="U2K71_001002"
     CDS             complement(15301..16023)
                     /locus_tag="U2K71_001002"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HEM7315105.1"
                     /translation="MVTPKYQRVVLKLSGEALAGDAGFGIKPPVIKEIIQEIKEVHEL
                     GVEMAIVVGGGNIWRGQIGAQMGMERAQADYMGMLATVMNALALQDTLENVGVPTRVQ
                     TSIEMRQIAEPYIRRKAERHLEKGRIVIFAGGTGNPYFSTDTTAALRAAEIGADVILM
                     AKNNVDGVYSADPKVDANAVKFEELTHLEVIAKGLQVMDSTASSLSMDNDIPLLVFNL
                     NEHGNIRRAILGENIGTTVRGK"
     gene            complement(16159..17040)
                     /locus_tag="U2K71_001003"
     CDS             complement(16159..17040)
                     /locus_tag="U2K71_001003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HEM7315106.1"
                     /translation="MADVTAKMVKELREMTGVGMMDAKKALVEVEGDMEKAVDLLREK
                     GMAKAAKKNDRIAAEGLASVAVKGNTAAIVEVNSETDFVSKNEMFQDLVKEIAELVAE
                     NKPADMEAAMKIKTDKGTIESDLIEATQVIGEKISFRRFEVVEKDDNAAFGGYLHMGG
                     RIAVLTVLDGTTDETVARDVAMHVAAINPRYVNESQIPEAELEHEKTVLTEQALNEGK
                     PANIVEKMVEGRLKKFKAEIALVDQPFVKDPDMTVEKYVASKGATVKTFVRFEVGEGI
                     EKREDNFVEEVMSQVKK"
     gene            complement(17139..17921)
                     /gene="rpsB"
                     /locus_tag="U2K71_001004"
     CDS             complement(17139..17921)
                     /gene="rpsB"
                     /locus_tag="U2K71_001004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HEM7315107.1"
                     /translation="MAVISMKQLLEAGVHFGHQTRRWNPKMKKYIFTERNGIYIIDLQ
                     KTVKLVDAAYDYMKSVAEEGGVALFVGTKKQAQEAIKEEATRAGQYFVNHRWLGGTLT
                     NWDTIQKRISRLKQINAMEEDGTFEVLPKKEVAGLNKERERLEKFLGGIADMPRIPDV
                     MYIVDPRKERIAVQEAHKLNIPIVAMVDTNCDPDEIDVVIPSNDDAIRAVKLITSKMA
                     DAFIEGNQGEDQVVEEDFTEENTATSIEEIVDVVEGDNSSAE"
     gene            18280..18759
                     /locus_tag="U2K71_001005"
     CDS             18280..18759
                     /locus_tag="U2K71_001005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArgR family transcriptional regulator"
                     /protein_id="HEM7315108.1"
                     /translation="MRKAQRHLLIKQVIEEFPIRTQEELLNKLGHYGVTATQATISRD
                     IRDLKIVKAPDENGVSRFVLFREKGTPETRTEEEDRLVQMIEDIVLKVERVHFLTIVN
                     TLPDNAHLFASVLDEIKPPSIVSTIAGFDTIMVISENEEDAKRVEEFLHHPTEVSLF"
     gene            18976..20067
                     /gene="phnW"
                     /locus_tag="U2K71_001006"
     CDS             18976..20067
                     /gene="phnW"
                     /locus_tag="U2K71_001006"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HEM7315109.1"
                     /translation="MYKLLTSGPLTTSETVKNAMLIDHCTWDQEYKEITQWIRKKLLD
                     LAQVSEGIYTTVLMQGSSSFGVESVLSSVIRPEDTVLICSNGAYRQRMINMCECLKLN
                     YTVYEVSEHQILQAHVITQLIEENPSISALAMVHSETTSGILNNLEEIAAVTQERDLL
                     FIVDAMSSFGGIPICVAELGIDFLISSSNKCIQGVPGFSFVLANKAELEKRKNVSRSL
                     SLDLYDQYRVMENENGKWRFTSPTHTVLAFQQALKELEAEGGIETRYSRYLANNHVLR
                     QKMTEMGFQPFIQEYQGPFITSFLYPKDWRFDFNHFYQYLKQNGFVIYPGKISDHECF
                     RIGNIGEIYPEDILELTAIIKDYKENVND"
     gene            20060..20188
                     /locus_tag="U2K71_001007"
     CDS             20060..20188
                     /locus_tag="U2K71_001007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEM7315110.1"
                     /translation="MIKTVIFDWAGTTVDFGCMAPVHAFRNAFLEKGIQLTDKEIR"
     gene            20192..20737
                     /locus_tag="U2K71_001008"
     CDS             20192..20737
                     /locus_tag="U2K71_001008"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HEM7315111.1"
                     /translation="MGKLKWDHIQQLLSLPSVKKQWIAIYGQLPQEKEVEELYQQFEH
                     YLFKDLVKHSTLKPDTLETIERLRNAGINIGSTNGFNAPMMTIVAETAKKERYSPDFM
                     ATFEDVEGYGRPFPYMIHKNMQYFKNKSVDEVIKVGDTVSDIQEGKNAGVLTVGVIEG
                     SSLMGISYDEYQKLEMGKKIV"
     gene            complement(21191..22882)
                     /locus_tag="U2K71_001009"
     CDS             complement(21191..22882)
                     /locus_tag="U2K71_001009"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HEM7315112.1"
                     /translation="MNNKDLVAKAVYDAVKDDLTLEQVEQLLENPKSAEHGDVAFPAF
                     SLAKVYRKAPQQIAADLAEKIDSANFEKIEVVGPYLNFFMNKELISKKVLQTVVKEKE
                     HYGDSNIGNQGTVPIDMSSPNIAKPISMGHLRSTVIGNSIGFIMEKIGYQPIRINHLG
                     DWGTQFGKLIVAYKKWGTEEAVKAEPINELLRLYVQFHEVAETEPELNEEARAWFKRL
                     EEGDKEAIQLWQWFRDESMKEFNKIYDLLEVRFDSLNGEAFYNDKMDEIVKLLEEKHL
                     LNEDKGAEIVDLSAYDLNPALIKKSDGATLYITRDLAAALYRKRTYDFKQSLYVVGNE
                     QSYHFKQLKAVLKEMGFDWSDDMHHIPFGLITQGGKKLSTRKGKIVLLEEVLNEAIQS
                     AKEQISEKNPDLENKDAVAKQVGVGAVIFHDLKNDRLNTFDFNLEEVVRFEGETGPYV
                     QYTHARAVSLLEKAGFVPSETADYALNDDTSWEVVKLVQKYPETVLSAGEKYEPSVIA
                     KHAIKLAQAFNKYYAHTKILADDEQKEARLALVYAVTVLLKEDLRLLGLHAPDKM"
     gene            complement(23302..24249)
                     /gene="arcC"
                     /locus_tag="U2K71_001010"
     CDS             complement(23302..24249)
                     /gene="arcC"
                     /locus_tag="U2K71_001010"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HEM7315113.1"
                     /translation="MAKRKVVVALGGNAILSTDASAKAQQEALMETAKYLVKFIEQGD
                     ELIISHGNGPQVGNLLIQQQAADSEKTPAMPLDTCVAMTEGSIGYWLQNAMGEVLKEK
                     GIDKDVVSLVTQVIVDENDPSFKNPSKPVGPFYTEEEAKEQMNADSTVTFKEDAGRGW
                     RKVVASPKPISIKEARVIETLVDQGVITVSVGGGGIPVVETATGLEGREAVIDKDFAS
                     EKLAEIIDADLLIVLTGVDNVYVNYQKPDQKKLETVTVSEMKQYIDEKQFAPGSMLPK
                     VEAAIAFVEAKPNAKAIITSLENIENLLASEEGTIIVAD"
     gene            complement(24364..25383)
                     /gene="argF"
                     /locus_tag="U2K71_001011"
     CDS             complement(24364..25383)
                     /gene="argF"
                     /locus_tag="U2K71_001011"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003073325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HEM7315114.1"
                     /translation="MKESVFQGRSLLAEKDFTKEELQYLIDFSEHLKDLKKRGIPHHY
                     LEGKNIALLFEKTSTRTRSAFTTAAIDLGAHPEYLGANDIQLGKKESTEDTAKVLGRM
                     FDGIEFRGFSQKMVEELAEFSGVPVWNGLTDEWHPTQMIADFLTIQENFGTVEGITVA
                     YCGDGRNNMANSLLVTGAILGANMRIVAPKELQPEEEIVKMAEGFAEKSGAQLMITDD
                     VDKGVDGADVLYSDVWVSMGEEDKFEERIKLLKPYQINMEMVEKTHNTDRLIFLHCLP
                     AFHDTNTVYGEQMKERFGITEMEVTDEVFRSKYARQFDQAENRMHSIKAIMAATLGNL
                     FIPRV"
     gene            complement(25474..26703)
                     /gene="arcA"
                     /locus_tag="U2K71_001012"
     CDS             complement(25474..26703)
                     /gene="arcA"
                     /locus_tag="U2K71_001012"
                     /EC_number="3.5.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase"
                     /protein_id="HEM7315115.1"
                     /translation="MDKPIHVFSEIGKLKTVLLKRPGQEVENLTPDIMDRLLFDDIPY
                     LPIAQEEHDNFAKTLQNEGVETLYLEKLAAEAIDAGNVKEQFLNKMLDESHIASNAVR
                     EGLHEFLLSMETQEMVDKIMAGVRTKDIEVRSSSLYDLSADDDYPFYMDPMPNLYFTR
                     DPSASMGNGMTVNKMTFEARRRESMFTEYILKHHPRFANKGIEVWLDRENPDHIEGGD
                     ELILSDKVVAVGISQRTNAKALEKLARHLFAKNSGFEKVLAIKIPNNRAMMHLDTVFT
                     MVDHDKFTIHPAIQSKDGKMDVFTIEPDGDDIKITHSDNLHETMKAALGLDDLVLIPT
                     GNGDEIVAPREQWNDGSNTLAIAPGVVVTYNRNYVSNELLRSYGIKVLEINSSELSRG
                     RGGPRCMSQPLVREDLK"
     gene            27164..27865
                     /locus_tag="U2K71_001013"
     CDS             27164..27865
                     /locus_tag="U2K71_001013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM7315116.1"
                     /translation="MRVNKIDFHFSKLRYQPDFEHFSDEEFQQIKEHTVLRSYKKGQI
                     LFDDGDPRQRLYILTKGLVRLERYDESATYYYYDYVNTNNLFPMSGMFGEETYTYTAQ
                     AMTDIETFYLPVSYYEKLARKNPEQLIYMIQKISKVVEIHELRIQIGLTSSAFDRVKQ
                     NLFILKEELGVPTKDGHITIPYPITLKELAVNSGTTRETAGQVIKQLRDSGLLDYDKK
                     RFTFCKTATTPAMRE"
     gene            complement(28474..29490)
                     /gene="tsaD"
                     /locus_tag="U2K71_001014"
     CDS             complement(28474..29490)
                     /gene="tsaD"
                     /locus_tag="U2K71_001014"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HEM7315117.1"
                     /translation="MNEELILAIESSCDETSVAVVRNGTEILSNIVASQINSHKRFGG
                     VVPEVASRHHVEQITLCLEDALVEAGVSAEDLSAVAVTYGPGLVGSLLIGISAAKAFA
                     WAHQLPLIPVNHMAGHIYAARLVKPFQFPLMALLVSGGHTELVYMQEDGSYEIIGETR
                     DDAAGEAYDKVGRVLGLSYPSGKEIDQLAHQGKDNYHFPRAMIHEDNYDFSFSGLKSA
                     FINLVHNAQQRGEDLDKNDLAASFQASVIDVLITKTLRACQNYPVKQLIIAGGVAANQ
                     GLREGLQDALAAKLPEVELVIPPLRLCGDNAAMIGAAAHVEMQKRNFASYQLNADPSL
                     VLSE"
     gene            complement(29487..29951)
                     /gene="rimI"
                     /locus_tag="U2K71_001015"
     CDS             complement(29487..29951)
                     /gene="rimI"
                     /locus_tag="U2K71_001015"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEM7315118.1"
                     /translation="MICRKKDFDEAYLAECVWMISENAYPHGSPWTKMQFLADIQQPH
                     SDYLLLAEDNQICGFIGYSKVIDEVEITNIAVAVSEQGKGHARHLLQLLITEQIKDSL
                     HVFLEVRLSNEAARKLYESEKFRILGKRKSYYRNPTEDAIIMSRKLKTETMK"
     gene            complement(29958..30500)
                     /gene="rimI"
                     /locus_tag="U2K71_001016"
     CDS             complement(29958..30500)
                     /gene="rimI"
                     /locus_tag="U2K71_001016"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HEM7315119.1"
                     /translation="MLKKFRAYARSFFQDKMIYTEKTVEQIDRRYVLRELTTEDIKEL
                     LDVEREVYNGELPWTKSAFISELKSPLPHLYLGIFDNGRMIGFIGSRAFGLDCHITNI
                     AVRPAYQRKRIGSLLIDEIENFAIMNRCETMSLEVRMSNQDAQRLYRKLGFQATTIKK
                     RYYTETKEDALDMIKLLENE"
     gene            complement(30484..31206)
                     /gene="tsaB"
                     /locus_tag="U2K71_001017"
     CDS             complement(30484..31206)
                     /gene="tsaB"
                     /locus_tag="U2K71_001017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HEM7315120.1"
                     /translation="MITLGIDTANQTLAIGVVEDEQILGQIQTNIKRNHSVTLMPAID
                     QLFADLQISPKDIDRIAVSDGPGSYTGLRIGVTTAKTIAYTLDKELVGVSSLKTIAAN
                     CIGVDNIIVPMFDARRKNVYAGAYRWKENRLETVLEDVHISASDLFTQLQKLNEAIYF
                     VGSDCHKFFSEIKEILPSAMINTVPAWDIPSGVTVAQLGALVEPVAEKQAFLPRYLKR
                     VEAEEKWLETHTPGDESYVEKI"
     gene            complement(31397..32377)
                     /locus_tag="U2K71_001018"
     CDS             complement(31397..32377)
                     /locus_tag="U2K71_001018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7315121.1"
                     /translation="MATIKDIAQLAGVSPATVSRVLNYDPDLSVGLATKQKIFEAAER
                     LNYTKHRKNAANTKQTLRLIQWYDDTEELEDLYYLSIRLGIEKKAEEANVTLLKETWD
                     SISDLPADGTIALGKFDEAQLLEMKGSQNALLLVDSDGTSQGIDSLVVDFKSSVQKVI
                     EHFVSHKAQHIAMLAGTEYTKKNHQRLKDPRIMAFQQVLAEKGYDEGPIIEADFSVES
                     GYQAVKQYLKTNPHVPDALFAANDALAIGALKAIQEKGLVVPEDISVMGFNDVSVAKY
                     VTPSLSTVKVYTEWMGELAVETILQLSNSKAPVSRKIIVETELIIRESTK"
     gene            complement(32401..33885)
                     /gene="galT"
                     /locus_tag="U2K71_001019"
     CDS             complement(32401..33885)
                     /gene="galT"
                     /locus_tag="U2K71_001019"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HEM7315122.1"
                     /translation="MSISQTICDFATLAIEAGGWMEMDRLYLQNRLLGMVGEDALESI
                     EVRPVKEESVDLLDQLVAKARENGVLSELPAEQEIFEAQLMDFLTPPPSVVNAFFAQH
                     YSKSPEEATEYFFNLSKKNDYIKTRAIAKNIKFPAESEYGELEITINLSKPEKDPKQI
                     AAEREAVKVDYPTCMLCMENEGYRGRLNYPARTNHRIIRMNLDGESWGFQYSPYAYYN
                     EHSIILSEQHRPMKITKETFARLLRITEVLPHYFVGSNADLPIVGGSILSHDHYQAGR
                     HEFPMAKAPIEKLIKLEDYPQVIAGVVKWPMSVIRLQSTEKNLLIEAANSVLEKWKAY
                     SDETVSIRAFSADGTPHHTITPIARRRDNYFELDLVLRDNNVSEEHPDGIFHPHKDVQ
                     HIKKENIGLIEVMGLAVLPPRLKPELVEVEHFLLGEENQMAEYHRPWAEQLKKEHPDL
                     TKDTVTDVVQKSVGQVFARVLEDAGVFKRDEKGQAAFERFTAIL"
     gene            complement(33897..34886)
                     /gene="galE"
                     /locus_tag="U2K71_001020"
     CDS             complement(33897..34886)
                     /gene="galE"
                     /locus_tag="U2K71_001020"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288452.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HEM7315123.1"
                     /translation="MTILVLGGAGYIGSHAVDQLVQKGYQVAVVDNLLTGHKQAVHPD
                     AHFYEGDIRDKEFLRSVFEKEPIEGVIHFAASSLVGESVEKPLMYFNNNVYGMQILLE
                     VMHEFNVNKIVFSSTAATYGEPKESPITEDTPANPKNPYGESKLMMEKMMKWCDQAYG
                     MRYVALRYFNVAGAKADASIGEDHTPETHLVPIILQVALGQRKALAVYGDDYDTPDGT
                     CIRDYVQVEDLIAAHILALEYLKEGNESNFFNLGSSKGYSVKEMLEAAREVTGKEIPA
                     EIAPRRAGDPSRLVASSEKAREILGWKPEYTDIKAIIKTAWDWHVSHPNGYEE"
     gene            35134..35301
                     /locus_tag="U2K71_001021"
     CDS             35134..35301
                     /locus_tag="U2K71_001021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3042 family protein"
                     /protein_id="HEM7315124.1"
                     /translation="MKKFVSGILVGSLATAAAVAGLVASVKKTVIDPIDEKEAMIEEN
                     RKKAMRKRVSR"
     gene            complement(35363..37174)
                     /locus_tag="U2K71_001022"
     CDS             complement(35363..37174)
                     /locus_tag="U2K71_001022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD/NAD(P)-binding protein"
                     /protein_id="HEM7315125.1"
                     /translation="MKIGIIGAGPRGLSVAERILHNNQDRHSLELFLFDPNGPGGRVW
                     RLNQPPELLMNSVSQQVTLFTDETLSSGGLVTQGPNLFQWSKGPATDYIEQTVTKNKQ
                     LIIEEINRLEKDQQSTRCLYGLYQRWFFSKLQEEFPASIQFVYSLVKKVSKRKDGYLL
                     QTDQQSYSIDQLVLASGHWENDETEEERVWVDYAKKNVLLYQLPSNPADVDLSKIPPK
                     ENVFLRGLGLSFFDYVGLFTVSRGGRFENEKGKLVYHPSGKEPIVYSGSRRGLPYYPR
                     GRNQKQGGAMAWPRLLTKENLEQWHQSGFLTGETFFDYLKKDVELFYYKKIIEEYHLP
                     IPKKNFEHDFLSLSSEECLGKHPALIPYRWDWSYLETPLAYQNEPFSEASRIFIEGQI
                     KEAEKGNCTGAFASTFDALKDWRDPIRQAIEWHVFSAKEYKEILWGWFTHLNSFLTIG
                     PPIIRTKELAALIDAGIFHLIEPPFDIQQKHNRYVISLQDKEISSRYLIEARLPQTSF
                     SRSSNPVIKNLKENKLVRPFSFSDNTGSYHSEAIDVERGTNRVIDASGEIQSDFYCYG
                     IPVEGVDWLTAAVARPYTDPWNLRQADKIAQLILTDK"
     gene            complement(37171..37536)
                     /locus_tag="U2K71_001023"
     CDS             complement(37171..37536)
                     /locus_tag="U2K71_001023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HEM7315126.1"
                     /translation="MIKLKRVYLDAEKEDGYRILVDRFWPRGISKEKAQLDLWLKEIA
                     PSNEARKTFDHVPERFASFAKKYQQELIFEEEKKQAIFKLSQLMKEHPVITFVYGAKN
                     EHQNHAVLLKKFIEMEACK"
     gene            complement(37699..38094)
                     /locus_tag="U2K71_001024"
     CDS             complement(37699..38094)
                     /locus_tag="U2K71_001024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEM7315127.1"
                     /translation="MVLWVINIILLLIVLAIILWEVYLRVMAKRSATTLTEEEFREGM
                     RKAQVIDVREKDVFDAGHILGARNIPYTVLKDSLGSIRKDQPVYIYDQKKSLSIRTAN
                     KLRKAGYQEIYLLKGGYDGWSGKIKSKKV"
     gene            complement(38112..39074)
                     /locus_tag="U2K71_001025"
     CDS             complement(38112..39074)
                     /locus_tag="U2K71_001025"
                     /EC_number="2.7.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family glucokinase"
                     /protein_id="HEM7315128.1"
                     /translation="MDKKIIGIDLGGTTAKFAILTPEGEIQQKWSIDTNILDDGKHIV
                     PEIIESINHRLNLYNMKAEDFIGIGMGTPGSVDSEAGTVIGAYNLNWTEVQFVKKLIE
                     AGTGIKFAIDNDANVAALGERWKGAGENDPDVVFVTLGTGVGGGIVAGGNLIHGVSGA
                     GGEIGHITVDPEGFECTCGKRGCLETVSSATGVVRLGRFLAEEYAGDSKLKAMLDNGE
                     EVTSKDIFEMAQEDDPFALMVVDRVCFYLGLACGNLGNTLNPSSIVLGGGVSAAGEFL
                     RSRVEKYFKEFTFPQVRESTKIKLAELGNEAGVIGAASLALKFA"
     gene            complement(39074..39286)
                     /locus_tag="U2K71_001026"
     CDS             complement(39074..39286)
                     /locus_tag="U2K71_001026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgQ family protein"
                     /protein_id="HEM7315129.1"
                     /translation="MESLYDVQQLFKKFGIFIYVGARIYDIELMTIELRNLYDSRLID
                     HDTYMTAWRILKREHRIEESRKKGNL"
     gene            complement(39307..40005)
                     /locus_tag="U2K71_001027"
     CDS             complement(39307..40005)
                     /locus_tag="U2K71_001027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HEM7315130.1"
                     /translation="MNYQQQIKLRAWLRRPLWTYAFLGIQTAVFLMMELFPRLEIPYY
                     AGMYGPYLVHFNEWWRLVTPIFIHFGLMHFVMNSLILYFMGQQIEAIYGHWRFFLIYM
                     FSGIMGNTASFAFNEANVLSGGASTSIFGLFGALFILGFHFKHNPAIQQLVRHFLLFI
                     VLTFVFGLLDTSVDVWGHVGGLIGGLVLGNVLGLPKQQTSYSIHQRILSTLVFVFLFV
                     ICILLGLKKYGLLV"
     gene            complement(40025..40567)
                     /locus_tag="U2K71_001028"
     CDS             complement(40025..40567)
                     /locus_tag="U2K71_001028"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HEM7315131.1"
                     /translation="MDQLKVRLRRKGIEKLKNLAKSGKKKEKEQRIYKQLFASEYWKS
                     AKVIGTTLSNEFELDTQPVIQRAEKEQKKIVVPKTLPKRQMAFYEMDEQSVLVRSSFG
                     VLEPQSEKLYLPEMIDLLIVPGIIYNRAGYRIGFGGGYYDRYLNNFHKKTCSLVFREQ
                     LNEQWQPESFDQKIQQLFID"
     gene            complement(40699..41703)
                     /locus_tag="U2K71_001029"
     CDS             complement(40699..41703)
                     /locus_tag="U2K71_001029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEM7315132.1"
                     /translation="MKKIYWTFYFLLFVLVILSIGSLMIGTPFIYLDQLFATFIGKES
                     SSLALIVREFRVPRLLISLLAGACLGVSGYILQGVTRNELADSSILGINAGAGFLVML
                     YLGFFSHYSLPFLLLVVAFLGGVLAACCVYLAAYDRNGFLGMNRILLSGIAVNAGLSA
                     LTLLCTIQLSKENYGFVNAWLAGSIWGASWPYVWALLPWAVILIPLGGLYAQRIVLLS
                     FGQERAQGLGLNVAKSQKILLLLAVALASGSVAVTGSLSFVGLLAPHAAKLVVRKENN
                     WTFVLSGLFGSIFLLSADMVARVILPSGEVPTGILIALFGAPYFLYALFIRQKHALNS
                     "
     gene            complement(41700..42689)
                     /locus_tag="U2K71_001030"
     CDS             complement(41700..42689)
                     /locus_tag="U2K71_001030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HEM7315133.1"
                     /translation="MRTMKKNRLLLLVCFVLLIIFFVALSNGAATVEKADFWQALFDF
                     DRSKQSHQIIRNIRLPRVAGAFLVGSCFALSGALMQGVTRNPLADSGLLGINAGASLA
                     MALSFAFWPKASDFSVFLMSLAGSFAVTLLVFGFLQFSKMGMNPLQLILAGVAISSFF
                     TAISQALTQFFNLQQDLAFWFVGGAANITWKQLAVLFPLYILAVLVSFSLGKAISLIH
                     LSETHALALGSHPKRTRLIVFCLVAVLAAIAVSLVGPVSFIGLLVPPVVRGMVGTDYR
                     YVLPVSFLVGGGLVMLADFTARMINPPFETPFGLVIALIGVPFLLIQVRREQR"
     gene            complement(42686..43492)
                     /locus_tag="U2K71_001031"
     CDS             complement(42686..43492)
                     /locus_tag="U2K71_001031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315134.1"
                     /translation="MTRLRAADIAAGYENKKIIENMSVTIPEGKITSLIGPNGSGKST
                     LLKSFTRILPVKSGKIIVNNSDISTYSSKELAKKIALLAQTSEHPTGMTVEEVVSYGR
                     YPYQKLFSGMDTQDHEAIEWALDATGLAGFRMRDIATLSGGQTQRVWIAMALAQETDI
                     LILDEPTTFLDPAHQLEILQLLTEINQMKQTTILMSIHDINHASRFSDYLLAMKNGKL
                     ITEGTPEEVITKECMKKIFEIDATIVTLPRTNKPVFLTYDLLPKEQEDQL"
     gene            43658..44614
                     /locus_tag="U2K71_001032"
     CDS             43658..44614
                     /locus_tag="U2K71_001032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM7315135.1"
                     /translation="MKLKTKVFTLSSILLLAGVLSACSPSSDKETATTANSTTSSNST
                     HTVTDTLGHKVEIPNAPKRIIGSYLEDYLIALGEKPIAQWTVGSGSIQDYLQDDLKDV
                     PTISYDLPYEKVLSFEPDLLLISSSATVEGGKYEQYSKIAPTYVVKNGTDVTWEEQLK
                     DVGKALAKEKEAEKIISDYQKSIKKTREELADKIENKTAAVLWVTNNSAFMVSKNRSS
                     GRIVYDDLEFGVPDLVEEVSKKATSDWSAVSSEKLAELDADYIILVNSDKDAAMFNEP
                     SWKNLKAVTDNHVLQFGPESSWLYNGPIASQEMVNDIKEKLN"
     gene            complement(44691..45209)
                     /locus_tag="U2K71_001033"
     CDS             complement(44691..45209)
                     /locus_tag="U2K71_001033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEM7315136.1"
                     /translation="MFISMWAQDKNGLIGKNGLLPWRLPNDMRFFREHTMDRILVMGR
                     KTYEGMGDLSLPYRHIIVLTTQPNFRTKENAEVMHSIDELLSYSQTISEDIYVSGGSR
                     IFQELLPYTGRIWRTLIDSTFEGDTYIGNIDFSDFALIEEYEGITDQENLYAHRFQKW
                     ERVKNRVASKEE"
     gene            complement(45297..45446)
                     /gene="rpmG"
                     /locus_tag="U2K71_001034"
     CDS             complement(45297..45446)
                     /gene="rpmG"
                     /locus_tag="U2K71_001034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM7315137.1"
                     /translation="MRVNITLECTSCKERNYLTSKNKRNNPDRLEKQKYCPRERKVTL
                     HRETK"
     gene            45674..46120
                     /locus_tag="U2K71_001035"
     CDS             45674..46120
                     /locus_tag="U2K71_001035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEM7315138.1"
                     /translation="MPWNMKDFPASMKNLDKLTRKKAIDIANALLDEGYPDNRAIPIA
                     IDQAKKWYEDASESEKRTFEKEKNPSKTDEHDTNPRAGKLLDSDVIVEYEEEQWIVKS
                     KGAKKASNHFDTKKEAIEKGKQVAQNKESTLFIYKKDGTKEKEIDY"
     gene            complement(46315..48210)
                     /gene="asnB"
                     /locus_tag="U2K71_001036"
     CDS             complement(46315..48210)
                     /gene="asnB"
                     /locus_tag="U2K71_001036"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HEM7315139.1"
                     /translation="MCGIVGFVNDKDNKKTIINTMMDRIVHRGPNSSGEYIDKHVALG
                     FRRLSIIDLEGGTQPIYNEDRTKIIIFNGEIYNYQPLREELIAAGHVFQTHADTEVLL
                     HGYEEWGTELLQKIRGMFAFAIWDNEKNELFGARDHFGIKPYYYAEMNGTFMFGSEIK
                     SFLPHPDFNKELNKEALKPYMTFQYSPLNGETFFKDVYRLPEGHYYTYKDGKIDIQQY
                     WDADFETKETHSRQEWIEKIDETVQASIEAHTVSDVEVGSFLSSGVDSSYVTSVLKPD
                     HSFSIGFDDKTYNEAIEARKLTELLDLDNTAAVIDGDMSFKAFPLIQYHLDEPDSNPS
                     CVPLYFLANLASQSVRVVQSGEGADELFAGYQTYGFHTNSKFIRVIAQGLKKLPKGTR
                     YNLGRKIGKMKNFHGRIHLYESLAPAKEYFIGHARVFEESEASEVLTPKYQTAPSVDE
                     IMTVHYEKTEGIKDEVNKMQYVDLHQWMPKDILLKADKLSMASSLEVRVPLLDIEVMK
                     LAQQIPSKFLLNQNNTKDIFRQAANKHLPEEWSNRVKLGFPVPIKAWLKEEHGYEQVK
                     ALFEADFAKEFFDQEKIMKLLDDHHEGRKEEQRKIWTIFSFLTWYKVYFVDETIPQAE
                     AIDYVTV"
BASE COUNT        13668 a        10349 c         8501 g        15963 t
ORIGIN      
        1 atttcacagg atttgcggga gtttttgatt agtctttgtt caagtcgcga agcaattcat
       61 cgatatggtt gccatagaca actgattcat caagttcaaa gaacaattca ggtgtcttat
      121 aaatacttag attgtgccct aactctcgac gaatcaatcc tgatgcttta ttcaatccat
      181 cttgtgctgt tttcttatct gatgctaagt cagataaaat gctgtaatag atcgttgctt
      241 gctgtaagtc ccccgtaact ttcacatctg tgatcgttac gttctccaca cgagggtcac
      301 ggaccttttt gcgcaaaata tcattgactt cttttaagat ttcttgaccg actcggcggt
      361 cacggtagtt agccataatg attggcctcc ttttatcagt ttttagtcat ttttgacttc
      421 ttccatgata aatccttcaa ttacgtcatc tactttgatg tcattgaatt tttcaatcat
      481 tgcaccacat tcataaccca ttttgacttc tttcacgtca tctttgaatc gtttcagact
      541 tgcaagttgg ccttcataga ttacgattcc gtcacggatt acacggacac cgctatctct
      601 acgaatatat ccttctgtta cataggcacc tgcaatcgta ccaactttag aaactttgaa
      661 ggtttcacga acgatcattt gaccggtgat tttttcttca aattcagggt ctagcatccc
      721 tttcatcgca gtttcgattt cttcgatggc tttatagatg atacggtgaa gacgaatatc
      781 gacttcttca gtatcagctt gcacttttgc ctgcggtgta ggacgaacgt tgaaaccgat
      841 gataatagcg ttgctggctg ctgctaaggt tacatcactt tcattgattg ctccgacagc
      901 tgaatggacg atttttacac ggacaccttc cacatcaatc ttcttcaatg atgcagccag
      961 tgcttctgct gaaccttgta catctgcttt gatgattacg ttaacttctt tcaattcgcc
     1021 ttctttcagg ctttcaaata agttatccaa agtcacacgg cttgtggcag cacggttttc
     1081 taataatgca cgtttcgcgc gttcttcacc cgctgcacgt gctgtctttt cgtcttcgaa
     1141 gactacgaaa cggtcacctg cttgaggtac atcattcaag ccagtgattt ctactggcgt
     1201 tgcaggtcct gcttctttat cgcgacggcc aagatcgttt gtcattacac ggacacgtcc
     1261 gtaagtatta ccgacaacga ttgggtcacc aacatgaagt gttccttctt ggacaagtaa
     1321 ggttgacaca ggacctttac ctttatcaag acgtgcttcg atcactgttc cgattgcgcg
     1381 ttgtgttgga tctgctttta aatcttctac ttctgcgact aacaggatca tttccagcaa
     1441 ttcttcgatg ttttggccga atttcgctga gatctcaaca aagatcgttt ctccacccca
     1501 tgcttcagga attaactcat actcacttaa ttcttgcata acatgttgag ggtttgcacc
     1561 tggtttatca attttattta cagcgacgat gattggaacg cctgcagctt tggcatggtt
     1621 gattgcttca actgtttgtg gcatgacgcc atcatctgca gcaacaacta agatcgtgat
     1681 atctgtgata cttgctccac gtgcacgcat acttgtaaag gccgcatgtc ctggtgtatc
     1741 caagaaagtg attggtttac catcaatatc gatctggtag gcaccgatat gctgtgtgat
     1801 tccgccggct tcgccgcttg ttacacgtga atggcgtaat gtatccaata aagttgtttt
     1861 cccatggtct acgtgtccca taatcgttac gactggcgga cgtgagacta atttatctgg
     1921 atcgacttct tctgcctcga agaatttatc gatatcagca atgtcaactt ggactttttc
     1981 ttgtggttcc atgccatagt ccgtcgctaa cagttcaatc gtatctttat ccagtgcttg
     2041 gttttgattg accatgacac caagcataaa taatttttta atgatttctg ctggctcacg
     2101 atgaattttt ttcgcaatat ctgctacgtt cataccttca gtatactcta atacatctgg
     2161 caattcacgg aatttgcgtg gtggtacagc tggtttagtt gattgttgct gtttgccttt
     2221 tttacctttt ttattataac gattacggtt attattatta aatttgccgc gattgttatt
     2281 gtttggtttg ttgttattgt tagtacggtt ttgattgccg ccttggctcg ttccttgacg
     2341 attttggctg ctggtatttc ccgtattatt ttggtttgtt gttggttttt ttccttgatt
     2401 cacttgaccg ctcccttgac cgcgatcttg ataactgcgg ttatttttat ttttttgttg
     2461 attgttcgtt gtttcttttt tacgttgatc gttcggctgt tgattggctg gttttctgct
     2521 tgcttgttgt gcagcttgtt gtttcgtgct tgggtttttg aacgcttgtt gcaatttctt
     2581 ctcatcattg ctagtaatcg cacccatatg gtttttcaca tcgatgccaa gttgttgtgc
     2641 tttttccacg acgtctttac tagatttttt gagttctttt gctaattcat agattctttt
     2701 gttgcccatg caatcacctt cctaaccttc aattagctcc ttggtcttct tcgcaaatcc
     2761 ggcatccatt attccgaata ccttgcgagg ttttccgatc atctgcgtga tttctgcttc
     2821 agaaaatagc tcaaagcaag gaacttcata atatgaactc ttatctttta tcttttttct
     2881 tgtattctcg cttgcatcgg ttgccacaaa caccaatttt gctttttgtt tgcggatttc
     2941 agcaatggtc agttcttcac ccgtgattag ttttcccgcg cgcatcgcta gaccgatgag
     3001 attcatggct ttttgccgat taactgtatt cattttccaa ataactcttt acgtgctttt
     3061 tggtgagtga catagtcaag aagttcttga taaaactcat ctgttaatgt tgtttcgagt
     3121 actcgatcga gaattttttt atcccaagct tgttgtactt cttccggctc aagtgagaca
     3181 tacgctcctc gtccaggcat ttttccagtt ggatcgatcg atacttcccc ttcttttgaa
     3241 cgggtgattc ggaccatctc ttttttaggt ttcatttcac ctgaaaccac ggatttgcgt
     3301 aaaggaattt tcctttgttt catctctttg cctccttact aaagttaagc ttctggtttt
     3361 tcttcgaccg tttcattgtt gaatgcaatt gtttcatatt catcgtctgt taaatctgac
     3421 tgaacgattg cttcatcatg cagttcttct gtttcttttt gttgttcttg attttcataa
     3481 aattccgtca tgtcagattc tgatttgata tcgattttat ggttagtcaa tttagcagct
     3541 aaacgtgcgt tttgtccgcg tttaccgatt gctagcgata attggtagtc tggaacgaca
     3601 actgtgcagg cctttggatt tgtctcatcg aagatcacat ccatgacttg tgcaggattc
     3661 aatgcatttg caatgaatac agctggatct tcattccatt cgacgatatc catgttttcg
     3721 ccttttaatt cattgacgat tgcttgaact cgttgtccct ttggcccgac acatgtaccg
     3781 actgcatcga tatttggatc atttgagcga actgccactt ttgaacgatc gccagcttca
     3841 cgggcgatgc tgacaatttc aacgattcca tcatagactt ctggtacttc ttgctcaaat
     3901 aagcggcgca ataggtctgg atggctgcgg ctaacaaata cttgcggacc tttagacgta
     3961 ttttctactc gggaaacata aactttgatt cgatcatgtg gttgataaaa ttcatttggc
     4021 atttgatctt gtttagaaag aactgcctca attttaccaa gattgacata aatataacgt
     4081 ttatcctggc gttcgacgat tccttgcatg atgtcttttt cataagcact gaattcgtta
     4141 taaataatcg ttctttctgc ttcacgtaca cgctgtaaaa taacttgttt agccgtttgc
     4201 gcagcgatcc gcccaaaatc ttttggtgtt acttcaaaac ggatcgtatc accgatctca
     4261 tatgctggat tgattaaaag tgcatctttc aatgagactt ccaactgtga atccatcact
     4321 tcttccgtta cttcttttac cgcatagaca tggatctttc cttttttctg atcaaattcc
     4381 acttcgacgt tttgtgcttg gccatagtga cgtttgtatg cagaaaccaa tgctgcttct
     4441 aaagcatcga tcacgatttc ttttgagatc cctttttcag cttctaaagc gtctaacgca
     4501 tttaacattt ctttactcat tttttctgat tcctctccgt gattaaaatt ggatagctaa
     4561 gcgagctttt gcaatatttt ttcggtcaaa agtcaattct ttttctcgtg tttttatgcg
     4621 tattttcaat gtaagctgtt cagcatcaaa tgattgaaga aatccttcat attgtttttc
     4681 gccatctact ggttggtata aggagacatg gacataatca ccaagtgcct tttcatagtc
     4741 gctttctttt ttcaatggcc gctctgctcc cggggaggat acctccagga aataagcttg
     4801 aggaatcgga tctggatctg ctgcatcaag tttttcactc agtttctcac tgacaaaggc
     4861 gcattcttcg atatcgatac cgccttcttt gtcaataaat actcgtaaga accagctttt
     4921 tccttctttt acaaattcga cttcgacaag ttcaaaattt tgttcatcta aaatcggtgt
     4981 caccatttct gtgactgttt caacgacgct actcaaaagt ttcgcctcct taaaaaatta
     5041 agtgcgtaat tccattaaaa agagtgagcg aaaaatccgc tcactcacag ttagtatcat
     5101 tacctataag aactatacca tactctgctt aaaattacaa gcagacagga aaacagatgc
     5161 ttatagcata tcgaataacg ataattgatt ttcatctggt agatctttca atacgccatt
     5221 gtcgttcata tattcgatca atgtttttga aactttaccg cgggtggcaa gatcttcttt
     5281 ggataaaaat ggaccgtctt ttcttgcttc aacgatttgt tttgccacgt tggttcccaa
     5341 acttgggaca gctctaaatg gtgcgatcaa cgtatcacct tcaataacaa agttaacggc
     5401 atccgattta tacaaatcaa tcattccaaa tttgaatcct cgttccagca tttcattggc
     5461 taactccagc actgtcagtt gattcttttc ttttacggaa gcatctaatc ctttgtccgt
     5521 gatttctttc attgcttgtt tcacagcatc ttttcctttg cacatggaga ctagatcaaa
     5581 gtcgtctgca cgtacagaaa agtatgcaca gtaatacaaa atagggaaat aaaccttgaa
     5641 ataagcgaca cgaagagcca tcaatacgta agctgcagca tgggctttcg gaaacatata
     5701 tttgattttt gaacaagaat cgatatacca gtcgggcaca ttattttctt tcatcgccga
     5761 tagataggtt tctcgcaatt catcagggat cttgttccat tgtcctttac gaacagtttc
     5821 catgatttta aatgccatcc cgctatctaa tccagcatgg atcaaataaa ccataatatc
     5881 atcacgacag ccgattactt ccgccagtgt cgcatctcct cgtcgaatca attcttccgc
     5941 atttcctaac catacgtctg taccatgaga tagtccggaa atttgcagta gttctgcaaa
     6001 agttgtagga tgcgtttctt ctagcatccc tcgaacaaaa cgagtgccga attctggaat
     6061 acctaatgtc cctgttttgg aataaatttg ttcctgactt actccaagta cttcaggacc
     6121 ggcaaatatc cgcatcactt ccgggtcatc cgtagggatc gtttgtggat cgatccctga
     6181 taaatcttga agcatccgaa taacagttgg gtcatcatgc cctagaatat ctaatttcaa
     6241 tacattatcg tgaatcgagt gaaaatcaaa atgagtcgtt ttccattccg aattttgatc
     6301 gtctgctgga tattggatcg gcgtaaaatc atagacatcc atataatcgg gaataacgat
     6361 gatcccccct ggatgctggc cggttgtccg tttgactcca gtagcgcctt ttgctaatct
     6421 gtcgacttct gcacttcgaa actgcagatt attgtccctt tcgtatcctt ttacatagcc
     6481 atatgctgtt ttgtcggcga ctgtaccaat cgttccggca cggtaaacat agtcttcgcc
     6541 aaacaatact ttcgtatagt tatgggcttc tgcttgataa tcacctgaga agttcaaatc
     6601 aatatcgggc actttgtctc catggaatcc taaaaatgtc tcaaatggaa tatcatgacc
     6661 gtctttgttt aagcgtgcac cacattttgg acattgtttt tctggcatgt caaatccaga
     6721 accatacgta ccatcttcaa aaaactcgga atattggcac tctggacaat aataatgagg
     6781 ggctaacgga ttaacttcag tgattcctgt catcgtcgca acaaagctag agccaacaga
     6841 gccacgtgaa cctactagat agccatcttc attactttta tgcactagtt tttgggcgat
     6901 cagataaata accgagaagc cattaccatt aatagaattc aactcttttt ttaatcgttt
     6961 ttgaatgatc tcaggtaacg gatctccgta catttgtttt gcttttgtgt aactcaattc
     7021 tgatatttca tcttctgaac ctggaatctt cggtgtataa agttcatctt tgactggaat
     7081 cacttcttcg cagatatctg cgattttatt cgtattttct actacgatct ctttcgctgt
     7141 ttcttcccct aaaaagtgaa atgcagttag catctcatcg gttgttcgga aatggacatc
     7201 cggcaaacta tgacgattca acggattcgc tccgcccata gaattgatca aaatttttcg
     7261 gtaaatggca tcttcttcgt tcagataatg tacattacct gttgcaacaa cgattttatc
     7321 caaacttttt ccgatttcta ccaaattttg gataatttct tccaaatcat gttcattttt
     7381 taccaattct tgctcgatca aaggcgcata gaccgctttt ggcataactt caatataatc
     7441 gtagaatttt gctcggtttc ttgcctcttc tacccctttt tgcatcatgg cttcgaatat
     7501 ttctccctta tcacaagctg agccgatcaa tagattttca cgcatctttt tcaattggga
     7561 gcgtggtatt cgtgggacgc gttcgaagta ttcgacattc gacattgaaa tcagtttgaa
     7621 caaatctttc agtcctgcct gattttttgc taaaatcgtt acatggaaag gtcgagccct
     7681 tttgtaagaa tctccttctc cgatatgctc atttagctga tcatgataaa gcatgttatg
     7741 attgtccatt gcctctttca caaagatcca tgctaagtga ccagttgctt cagcatcata
     7801 gattgctcgg tgatgctgtt ccaagctgac accaaatttt ttagataaca cacctaatcc
     7861 gaaacgtttg aattgaggat acagatatct agctaattcc aacgtatcaa tgactggatt
     7921 ggccgcttca ggaataccat accttgcgta gctagtattc aagaacccca tatcaaaagc
     7981 cgcgttgtgg gctaccaaaa ttgtatcttt tgaaaattca aggaacatcc gcaatacttc
     8041 ttcttccgac tttgatcctc gaaccattcc atccgtgata ccagtcagat ctaccgttgt
     8101 tcgtgacaaa ggatgtcctg gatcgataaa ttcatcaaag ctttcgatca catttccttt
     8161 atacattttg actgcggcta gctcgatgat cgtgtcatat actgcagaca atcctgtcgt
     8221 ttccacgtca aatacgacat atgtggcttc actcaatgct tcatgtgcat cattataagc
     8281 aattgggaca ccatcatcta cgacattcgc ttccacgccg tataagattt tcacacctgc
     8341 tttttttcca gcactatgtg cttccggaaa ggcttgcgca ccaccgtggt ctgtaatggc
     8401 gatagctcgg tgtccccatt tccctgcttg tgccacaaga tcagagatgc tgtttgttgc
     8461 atccatcgta ctcatattgc tatgaacatg aagctctact cgtttttccc cttcaggagc
     8521 ataatctttg cgaggtgtat gtttgacttc tatgatatct tgcgcattca tgaccaaatc
     8581 acggacaaac gtatcttctt gaatacttcc tcgtaccttt aaccagctac cgacactgat
     8641 cgcatcaaat acttgttcat ctttttcgcc gttcgagaat tttttgacga taaatgatga
     8701 agtataatct gtgattttca aagtcaagat cttgcgtttt gaacgtagtt ctctgacttc
     8761 tttgtcgaat acgtacccct caagtgtgac tcttcgttct tcttcaacga tactgatcat
     8821 tggtgtcgtt ggttcatcgg ctggaatatt tcgtcctagc tggatgtgga tgggtccttc
     8881 aagagctggc gattgctctt ttctttcctt tttcttttgt tcgtggacaa ctagactttc
     8941 agctgcttgc ttcataaagg cttctgcctg ttcttgtttg cgttcttcaa aaagtttcaa
     9001 tactcgttcc gcttgctgct catccacttc tggttcgata cgaaattttg ggaatccgta
     9061 actgacgaac aactcttcta ctaatggcaa gtattgttgc ttcaaataag aaatagctcc
     9121 tgtactgtcg attggcaaga tgactttacg atcttttatc acaggtacct gcgttttcaa
     9181 tacttgctgt gctagtggtg tatcgcattg ctgattttcc aaagcttggg cccagtaatc
     9241 ttgcaagagc tgttcatcga atgtctggtc atctgctaag atattcagtt tgacttgtgc
     9301 aatatcttta aaagcgatag tcagatgctg agtcaatgac cgatatagca taataggcaa
     9361 aattttggaa aaatgcagcg taaattccca taaacgcgat ttacgatgga ccactacact
     9421 atccatttca ccagcagtaa gcagtggatg atttttttct gcttcctcta gttggatttg
     9481 ttccaacagt ttttcaaata aatctcgttt ttctgacaaa gatatacgcc ctttctgatg
     9541 agaaacaatg caattgatcg catcttatcc tcttcttttt cagtaccttt tagtatactc
     9601 gttttttctg agttttacat taaaaaattg gaactgcgat tcaaatcgca gttccaattt
     9661 taaaagcgaa tcatcagatt gtcaaaaaca gctgattcag ctatttttat tttctgtgtt
     9721 ttaacgctta ttattcgcct tggttcaaga ggattggtaa agtattcacc aattcttctt
     9781 tacgtacttc aaccatttcg ccagttttct tgattttcac ttctacgatt ccatccactg
     9841 cttttttacc gacagtgatt ctgattggac agccaatcaa atcagaatcc gcgaatttca
     9901 ctcctgcgcg ttcatttctg tcatcaacca gtacttcata gccagctttt gtcatcgctt
     9961 cttctagttc attcgtcagg tttgtttggt attcgtcttt tagattcatt tggacgatat
    10021 gcaaatcaaa tggcgcaatt ccttttggcc agttgattcc ttgttcatct gaatgttgtt
    10081 ctacgattgc tgataataaa cgacttacac cgataccata acagcccata atcacaaatt
    10141 tttctcgtcc attttcgtct agtacagttg ctcccataga ttcactgtaa cgagtgccga
    10201 ttttgaagat atgtccgatt tcgattcctc gagtaaaggc aagaacacca ttgttgtctg
    10261 gtgaaggatc tccttcttgt acaaaacgaa gatcttcata agaaagtaca gtgaaatcgc
    10321 gttctggatt cacattttta tagtggaatc ctgtttcgtt cgctccagta atggcattcg
    10381 ctaaatcttg gacataacgg tctgcatata cttttacgtc atctgaaaca ccgactggtc
    10441 cgacagaacc aaactcagca cctagatatt tttgtgcttc ttcttctgtt gcttcttcta
    10501 aaaagtcagc agataaaaag tttttcagtt ttacgtcatt gacttcatgg tctccgcgta
    10561 ccaaaacaag gacaggttgt tcatccgcaa tgaacaatac agacttgatg attttttgag
    10621 gttcaacaga aaagaaatct gctacttcgt caattgattt tacgttaggt gtagctactt
    10681 tctccaattc aagctgtgtt tcatgcgatt tttttgatac atagtagctt gtagccatct
    10741 ccaagtttgc agcataatca ctttcagtcg aataacagat cgtatcctcc ccgatttctg
    10801 agatcgccat gaattctttt gagtctttcc cgcccattgc gccaccgtct ccgataatcg
    10861 agcgaaattc taagccacaa cgcttgaata cttcggtgta ggctttttcg taatctttat
    10921 atgtctcgtc caaactttct tccgttgcat ggaaagaata cgcatctttc atgataaact
    10981 ctcttccgcg aagcaaaccg aagcgaggtc gtttttcgtc acgatattta ggttggattt
    11041 gataaagatt taaaggtaat ttcttatatg aattgatttc attacgcatc aaatcagtaa
    11101 aagtttcttc gtgagttggt ccaaggatca tttttcgatc attgcggtct ttgaaacgat
    11161 acaagtttgg tccatatgtt tcgtaacgcc cggattcttc ccaaagttct gctggcaaga
    11221 tggcaggcat cagcatttct accgctccga ttttttcaaa ttcttcccgc ataatgcgtt
    11281 tcagtttttc caatacacga tttgctaatg gcagatatga ataaataccc gctgatactt
    11341 ggcggatata tccagctctt agcaagattt gatggcttaa tacttctgca tcatttggaa
    11401 cttcgcgtaa tgttggaatc aacatttttg attgtctcat tctatatact cctttatttc
    11461 ctcaaaatag ttatcgtttg ttattatacc ctatttgtca actttttcta gaaaaagaat
    11521 cgttgaatat cgttccatgt aactagcacc atcaacaaca ttaaaaatcc aaagccagct
    11581 aatgtcaata ttccttcttt ttcttgactt aatggtttcc cgcgaatccc ttcaaaaata
    11641 ttcaagacta atttcccgcc atctaaggca ggaatcggaa gcagattgac gattccgaga
    11701 ttcattgaaa gaagcgccat caaactaata acagttgtta caccttgatt tgctgcttct
    11761 gaagacaatt ggtacatcat gacaggtccg cctaatttat ctaaactgaa acctgtaaat
    11821 agtgatccta aggctttgaa aatttccaat gaaccgctga aagcttgacg cgtccctcca
    11881 atgatcttat ccataaaacc tgtattcatt ggggctttta taccaagttg gccaactttt
    11941 tcgccatttg actctacaga tttcggtgtc acgtcgacag attgcacctg accttctcgt
    12001 tcgatcttaa agtctaatgt tttatccgga tttttcgtaa tgactgttgt cagatcattc
    12061 catgaatgga tttcttttcc gtccacactg actacctcat cattttcttt taagcctgct
    12121 tctgccgcag ctccattagg catgatctct cctacgcgat tagtgttcgt cacttgaaca
    12181 ccaccttgca taaatgccag aatgatgaat aacaggattg ccaaaataaa gttattcatg
    12241 ggtccagcaa agttggtcag catacgctgc cacaatttag ccgattggaa ctgtacgtct
    12301 tttggcgcaa ttctgatttc tgttccgtcg ttttcaataa tcgtcgcatc gtgatcaaca
    12361 gggtaggtta ctacttctgt ttcgtctcca ttcacataac cagtgatagt cagctcatct
    12421 tctaaatcat aacggctcaa ttccatcggg atagcatttg tcaattggat tttcttactt
    12481 aaatttatct tctctacgat gccgtcagaa tttagtaaaa gagaaagagg cattcctggt
    12541 gccatttctg tttcatcatc cccattgcct gccattcgaa cataaccgcc aataggtaat
    12601 aggcgaagcg tatatgttgt cccgtctttt gcttgatgcc cataaatttt cggacccata
    12661 ccgatagcaa attcgcgaac gaggattcct gatcgtttcg caaagaagaa atgaccaaac
    12721 tcatgaacaa tcactaatat accaaaaacg atgataaatg tcagaatcgt tttcatagct
    12781 tttttctcct ttacttgttt tttgtgtttt ttataaaaca acgataaaac actataattt
    12841 taccatagta taccaaagac accaatagta tcaatataat gaaaaataaa aacctgcgtc
    12901 ttttatgaaa ttcacaaaga aacgcaggta tacttactaa ataatgccaa ataaatgcat
    12961 gataggaaat acaaacagca aactgtcgaa acggtctaag attccaccat ggcctggtaa
    13021 gatatttccc gagtctttca catcataatg acgtttgatt gcagattcga ccaaatcacc
    13081 aaattgtccg actgctgaaa aaataatcgt catcagaagc atgacaaaca taccatgatt
    13141 aaatagttct tttgctgggt atagcaataa ataaagaaat gcgactacaa cagcactacc
    13201 aataccaccg agcgcacctt caatcgtttt gttgggcgaa atatcaggac ttaacttatg
    13261 ctgaccataa cgtcgaccaa ccatatatgc tccgatatca gttgcccaaa caataaatag
    13321 accaaatatc aaaacagcca agctttctgt tcgtgcattg acaaaattct ggaaacctat
    13381 tcccacatac aagctgacaa gcacaggaaa acctgcttca tcaatcgtat acgtattttt
    13441 agaaataact gatgttccta aaagaatcat aactataaga taaaataaca taaatgtact
    13501 tacattctct ggaagaaaga aaaaccatct ttcttttggc aaaacaagta acactgcacc
    13561 aacggcggat aaaactcctt caaaacttaa gagtgtcaat cctttcatcc gaaacagctc
    13621 gtaaactcct attactgcta agattgctgc tgtaagctca accgctatgc ctccgatcca
    13681 tataatcgga ataaacaaga ctagcgccac ggctgctgtg accacacgtt gtttcatgac
    13741 tgatcccctt tctcatcttc tgtttttttt acaccgccga atcgacggtc tcgattttga
    13801 taagaggcga ttgcttcttc aagatgcgca ccatcaaaat caggccaaag cgcttttgta
    13861 aaatacagct cactatatgc gatctgccat aacagaaaat tactaattcg ctcttctcca
    13921 cttgtacgga tcatcagctc aggatcttgt aactctttag gcaagaaacc tgtcattaaa
    13981 tgcttagcaa tcaattcatc atcgatttct tctgcgtgta tttctccttt tgctacttct
    14041 tcggcaattt cttggacagc agtcacgatc tctgctctgc ttccataatt caaagcaaaa
    14101 ttcaatacca ttccagtatt gttttttgtt tgctcaattg cacgtcttac agcatcttgc
    14161 gtatgttctg gcaatacgtt ttcataaccc atgacatgaa ctttgacatt ttctttaatc
    14221 aattctggta cgaacgtatc aaagaagtcg acaggtagtt gcatgagaaa acttacttca
    14281 tcttttggtc gcttccagtt ttcagtcgaa aaggcataaa gcgtcaacac tttcactcca
    14341 agccttgatg ctttttttgt gactttcttt actgtttcca tgccttcttt atggccagca
    14401 actcgtggca aacgacggtt ttgcgcccaa cgaccattgc cgtccatgat gatggcaatg
    14461 tgctgaggaa ttggtccatc ggtattaaat tgaaattcgc ttttttcttg aatatatttg
    14521 tttttttgcg gaaaaaaacg taacattctt tttcctccta tacttaaaag attcccacta
    14581 tcttaagtat tatagcaata tctaactgtt tttcctatga aatcattgtt tttttattta
    14641 aaaactgaat aattttttct ttttttaatt tttaaataaa agaaatataa ataaaaacag
    14701 aggaagcgga atgatccgcc cctctgtttt tatgatttta ttaaacttcc aataattctt
    14761 tttctttatc agcagcgatc ttatctactt ctttgatgct gttatctgtc aatgtttgta
    14821 cgtctttttc taatccgcgc aaatcatctt cggtaatatc accatttttt tgttgttttt
    14881 tatattcatc gatcgcatca cgacggatgt tacgaatcgc gatttttgca ttttctgctt
    14941 cttttttaac atctttagct aattcttttc tgcgttcttc tgtcagttgc gggataacta
    15001 agcgaatgac atttccatca ttggttggac tgattccgat atcacttgcc ataatagctt
    15061 tttcaacatc ttgaagaatg ctcttgtcaa aaggtgtgat cattaaaaca cgtgcttctg
    15121 gaatttggat tgatgccatt tggttcaatg gtgttggcgc accgtagtag ttcacagtaa
    15181 tacggtccaa aaggctagcg ttcgcacggc ctgcgcggat ttgcccaagt tcacgttgaa
    15241 gattttgtgc tgcttttttc attttttctt ttgcttctgt cataattgca tctgccattt
    15301 ttatttcccc cttacagttg ttccgatatt ttcgcctaag attgcacggc gaatatttcc
    15361 gtgttcattt aaattgaaga caagcagtgg aatatcatta tccatgctta gtgaacttgc
    15421 agttgaatcc atgacttgta atcctttggc aataacttcc agatgagtca gttcttcaaa
    15481 ttttactgca tttgcatcta ctttcggatc agcagaataa acaccgtcta cattgttttt
    15541 agccatcagg ataacatctg cgccgatttc tgccgcgcgt aatgctgctg ttgtgtctgt
    15601 cgagaagtac gggttgccgg taccgcctgc aaagataacg atccgtcctt tttctaaatg
    15661 acgttctgct ttacggcgaa tatatggctc tgcgatttga cgcatttcaa tcgaagtttg
    15721 cacgcgtgtc ggtacaccca cgttttccaa cgtatcctgt aaagctaagg cattcataac
    15781 tgtagctaac atacccatgt aatcagcttg agcacgttcc atgcccattt gtgctccgat
    15841 ttgtccgcgc cagatatttc cgccgcccac aacgattgcc atttcaacgc ccagctcgtg
    15901 aacttctttg atttcttgga tgatttcttt gattactggt ggtttgattc caaatcctgc
    15961 gtccccagct aaagcttctc cactcaattt taagactaca cgctgatatt tcggtgtgac
    16021 catgttcatt cctccaatat tttctattgc cattttacca tacttagatg atttcgacac
    16081 tctaagactg gtttttcttg ttttgcttaa aaaaagggag cgcacaaacc gtgcgttccc
    16141 gtctgttatt caacagaatt attttttcac ttggctcata acttcttcta caaagttatc
    16201 ttcgcgtttt tcgatacctt cgccaacttc gaaacgtacg aatgttttaa cagttgcgcc
    16261 ttttgatgca acgtattttt caacagtcat atcaggatct ttaacgaatg gttggtccac
    16321 caaagcgatt tctgctttga atttcttcaa gcgtccttct accatttttt caacgatatt
    16381 cgctggtttg ccttcgttca atgcttgttc tgtaagaaca gttttttcat gttctaattc
    16441 agcttctgga atttgtgatt cgtttacata acgaggattg atagctgcta cgtgcatagc
    16501 tacgtctctt gcaactgttt cgtcagttgt tccatctaga acagtcaata cggcaatacg
    16561 tccgcccata tgcaagtatc caccaaatgc agcattgtcg tctttttcta caacttcaaa
    16621 gcgacggaag ctgatttttt ctccgataac ttgagttgct tcgatcaagt cagattcaat
    16681 tgtaccttta tctgttttga ttttcattgc agcttccatg tctgctggtt tgttttcagc
    16741 aacaagttct gcaatttctt ttactaaatc ttggaacatt tcgtttttag aaacaaagtc
    16801 tgtttctgag tttacttcaa cgattgctgc agtgttacct ttcacagcaa ctgaagccaa
    16861 tccttcagct gcaatgcggt catttttctt cgcagccttt gccattcctt tttcacgtaa
    16921 aagatcaact gctttttcca tgtcgccttc tacttcaact aatgcttttt tcgcatccat
    16981 cattcctacg ccagtcattt cgcgtagttc ttttaccatt ttagctgtaa cgtctgccat
    17041 gtttaactcc tcctgtgtgt ttttcttttt aaaaaaagct gcttcaaagg agagtgaggc
    17101 ttgtcgccca gcctttgaaa cagctccatc attcaaaatt attctgctga agaattgtcg
    17161 ccttcaacaa catctacgat ttcttcgatt gatgttgctg tgttttcttc tgtgaaatct
    17221 tcttcaacta cttgatcttc accttggttt ccttcgatga aagcatcagc catttttgaa
    17281 gtaatcaatt taacggcacg aattgcatcg tcgtttgaag ggattactac gtcgatttca
    17341 tctggatcgc agtttgtatc aaccatagct acgattggga tatttaattt gtgtgcttct
    17401 tgaacagcaa tacgttcttt acgtgggtca acgatgtaca ttacatctgg gattcttggc
    17461 atatcagcga taccgcctaa gaatttttca agacgttcac gttctttgtt caaaccagca
    17521 acttcttttt taggtagaac ttcaaaagtt ccatcttctt ccattgcatt gatttgtttc
    17581 aaacggctga tacgtttttg gatagtatcc cagtttgtca atgttccgcc taaccaacga
    17641 tggttaacga agtattgtcc tgcacgagtt gcttcttctt tgattgcttc ttgtgcttgt
    17701 ttttttgtac ctacgaataa tgctacgccg ccttcttcag caacgctctt catgtaatca
    17761 taagctgcat ctactaattt aactgttttt tgcaagtcaa tgatgtagat tccgtttctt
    17821 tctgtgaaga tatatttctt catttttgga ttccagcgac gagtttggtg accaaagtgt
    17881 acgccggctt ctagtaattg tttcatagaa attactgcca tgtttgtttc ctccgatttg
    17941 gttttatttt tcctcttctt atcttctacc ttgccggcaa actttttcaa gcacctaccg
    18001 acaatccaat aagaatgtgg atttgctgat gcataaagca ccctcagcta ttatacagac
    18061 aaaagatccg tatcgcaaga gcaaatatac ttttctgctc aacattcaat agtttacctg
    18121 aaagataaaa agaattcaag aaaaacagta gatttctctt tgtttcttta ttatcctttt
    18181 tccataaaat aattttaggt ataaaagacg aatattattg ggcaatcctt cttttttttg
    18241 ttatgataca tgtgaaatct ttcgtaaagg agaaagaaaa tgcgcaaagc tcaaagacat
    18301 ttgctgatta aacaagtcat tgaagaattc ccgatccgta cgcaagaaga actattaaac
    18361 aaacttgggc attatggcgt taccgccacg caggcaacga tttctcgaga tatccgtgat
    18421 ctaaaaatcg tgaaggcacc tgatgaaaat ggcgtttccc gcttcgtatt gtttcgcgaa
    18481 aaaggaactc ctgagactag gactgaggaa gaggatcggc tggttcaaat gattgaagac
    18541 attgtattga aagtcgaacg agtccacttt ttgacgatcg tcaatacctt gccagacaat
    18601 gctcatttat ttgcatctgt tttagatgag atcaaacctc cctctattgt cagtacaatt
    18661 gctggattcg atacgatcat ggtcatctcg gaaaatgagg aggacgcaaa acgagtagaa
    18721 gaatttcttc accatccaac tgaagtatca cttttttaat cagagcacgt attcctaata
    18781 tggaatgcgt gtttttttat tttcaatatt ttccttattg aataaacctt tacactcttt
    18841 acaaaaatac atcatttttt attcaaattt ttcaacacct tgattgaatc acaataatca
    18901 tcatttatat tggatgttaa caagagcagc ttcttgctct ttgtgtattt caaaaaagaa
    18961 atgaaaggaa atcatatgta taaattatta acttctggac cattgacgac ttctgaaaca
    19021 gtgaaaaatg ctatgctgat cgatcattgt acttgggatc aagaatacaa agaaattaca
    19081 caatggataa gaaaaaaatt attggatctt gctcaagtat cggaaggaat atacacaacg
    19141 gtcttaatgc aaggtagcag ctccttcggg gtagaatcgg tattaagttc tgtgatcagg
    19201 ccagaagata ctgtactgat ttgttcaaac ggcgcatata gacaacgaat gatcaacatg
    19261 tgcgaatgtc tgaaactgaa ctataccgtt tatgaggtat cggaacatca aatcctccaa
    19321 gctcatgtga ttacacagct gattgaagag aatccgtcta tctccgcact agcaatggtc
    19381 catagcgaga ctacttccgg tattttgaat aatttggaag agattgctgc tgtcacacaa
    19441 gaaagagatc ttttgtttat agtggacgcg atgtcaagct ttggtgggat accgatttgt
    19501 gtagccgaac tagggattga ttttttgatt tccagctcaa acaaatgtat tcaaggtgtt
    19561 ccaggctttt cttttgtact tgctaataaa gcggaactgg aaaaacgaaa aaatgttagc
    19621 cgaagtttgt cacttgatct atatgaccag taccgcgtga tggaaaacga gaatggcaaa
    19681 tggcgtttca cttcccctac ccatacggtt ttggcttttc aacaagcgct gaaggaacta
    19741 gaggcagaag gaggaatcga aactcgatat tcacgttatc tcgccaataa tcatgtgcta
    19801 cgacaaaaaa tgacagagat ggggttccag cctttcattc aagaatatca ggggccattc
    19861 atcacctctt ttctttaccc taaggactgg cgatttgatt ttaatcattt ttatcagtat
    19921 ttaaagcaaa atggatttgt gatctatccc gggaaaatca gcgatcatga atgttttcgt
    19981 atcggaaata ttggcgagat ctatccggaa gatattttgg aactgacagc aattatcaaa
    20041 gactataagg agaacgttaa tgattaaaac tgtaattttt gattgggcag gaacaactgt
    20101 ggactttggc tgtatggctc ctgtccacgc ttttagaaat gcttttctag aaaaaggaat
    20161 ccaactgact gacaaagaaa tccgctaacc gatgggaaaa ttaaaatggg atcatatcca
    20221 acagcttctt tccctccctt ctgtcaaaaa acaatggata gctatttatg gacaactacc
    20281 tcaggaaaaa gaggtggagg aattgtatca acaatttgaa cattatctgt ttaaagattt
    20341 ggtcaagcat tctaccttga aacccgacac attggaaacg atcgaacggt tgagaaacgc
    20401 tgggatcaat attggttcca cgaatggatt taatgctcct atgatgacaa tcgtagcaga
    20461 aaccgcaaaa aaagaaaggt attcaccaga cttcatggca acttttgaag acgtcgaagg
    20521 gtatggtcga ccgtttcctt atatgatcca taaaaacatg cagtacttca aaaacaagtc
    20581 ggttgatgaa gtaatcaaag tcggagatac ggtctcagat atccaggaag ggaaaaatgc
    20641 cggagtgttg acagtaggtg taattgaagg tagttcgcta atggggattt cttatgatga
    20701 gtaccaaaaa ttagagatgg ggaaaaagat cgtttaaaac agtctgttgc tcttagattc
    20761 caacaagccg gcgctgatat ttgtgtaaac aatttaaaag agttaacaga atacattttg
    20821 tacgctgaca tgaatccaga atgactttta tacaaaataa gaggttgtga aaaagctgtc
    20881 ctgtatcaag taataaaaac agccaagcaa aaatagctcc tcatattttt cgtttggtta
    20941 ggcttattac cgcagatgca agcttttgaa caccgtttat tcgaaaaaaa aaagggagct
    21001 gtgacacgac ttttgtcaca gtccccttat ttgcaaacaa acagtgttta agagacaacc
    21061 tctccactaa acggtgttca ttcagctgac tctcggtatt tgctcaaaaa acgataaaat
    21121 ttgaaaagct attttctctt ttgtttctct aatactcgga tcagaacgct gatttcacat
    21181 tctctttttc ttacatctta tctggtgcat gaagtcctag caaacgaagg tcttctttca
    21241 ataatacagt cactgcataa actagagcta agcgtgcttc tttttgttca tcatcagcta
    21301 aaatcttcgt atgagcatag tatttgttga atgcttgtgc taatttgatt gcatgtttcg
    21361 caatcactga cggttcgtat ttttccccag cagacaagac cgtttcaggg tatttttgaa
    21421 ctaatttaac cacttcccag ctggtatcgt cattcaatgc atagtctgct gtttctgaag
    21481 ggacaaatcc tgctttttcc aacaggctga ctgcacgagc atgtgtatat tgcacataag
    21541 gtccagtttc tccttcaaat cgaacaactt cttccaggtt aaagtcaaat gtattcagtc
    21601 gatcgttttt caaatcgtgg aaaatgactg ccccaacccc aacttgttta gctacagcat
    21661 ctttgttttc caaatcaggg tttttctcgc tgatttgttc tttagcagat tggattgctt
    21721 catttaatac ttcttctaat aagacgatct ttcctttacg agttgataat tttttaccac
    21781 cttgcgtgat caaaccaaat ggaatatggt gcatgtcgtc agaccagtca aagcccattt
    21841 ccttcaagac agctttcaac tgtttaaaat gatagctttg ttcattccct actacatata
    21901 gagattgttt gaagtcgtaa gtacgtttac ggtacaatgc agcagctaag tcacgtgtaa
    21961 tgtatagtgt cgcaccgtct gacttcttaa tcaacgctgg attcaggtca taagcagata
    22021 ggtcaacgat ctctgcacct ttatcctcgt tcagcaaatg tttttcttct aaaagtttga
    22081 cgatttcatc catcttgtca ttgtagaaag cttcaccgtt caacgaatcg aaacgtactt
    22141 ccagcagatc atagatttta ttgaattctt tcatcgattc atcacggaac cattgccata
    22201 attgaatagc ctctttatcg ccttcttcca gacgtttgaa ccaagcacga gcttcttcat
    22261 ttaattctgg ttctgtttca gctacttcat ggaattggac gtataaacga agcaattcat
    22321 tgattggttc tgctttaaca gcttcttctg taccccattt tttgtaagcg acgatcagtt
    22381 ttccaaactg tgttccccag tcacctaaat ggttgatgcg gattggctga taaccgattt
    22441 tttccataat gaaaccgatc gaattaccga taactgtcga acgcaaatgt cccattgaga
    22501 ttggtttggc aatattagga gaagacatgt cgattggtac tgttccttga ttcccgatgt
    22561 tgctatcccc ataatgttct ttttctttca ctactgtttg caaaactttt ttgctgataa
    22621 gttctttatt catgaaaaag ttcaagtaag gtccgacaac ttcgattttt tcaaagttgg
    22681 cagaatcgat tttttctgct aaatctgcag cgatttgctg tggcgctttt cggtaaactt
    22741 ttgctaatga aaaggctgga aatgcaacat ctccgtgttc agctgatttt ggattttcta
    22801 gtaattgctc gacttgttcc agtgttaaat catctttgac agcgtcatag acagcttttg
    22861 cgacaagatc tttattattc atctttattc ctcctaaagt gttcccttga ttgttaaaca
    22921 aaaaaaatct ctagcgacgg catacttagt atgccatcac tagagacgag atttcccgcg
    22981 gtaccactcc aattgtccta aaaaaggacc agctccatct gatatcggta gacgaccgtc
    23041 tgtttaaaac agaattctcc aagtgcgctt cgttgtatct tcttcattcg gcttccacca
    23101 cttccgaatt cgctagccaa gaatgataca agtactctct tgttcaacaa atcttagtct
    23161 tattaaactt tcttgaatta tatcagaaaa aaaaacgaaa ggctagtcgt ttcataaaaa
    23221 aacaatccag atggcctaaa ggagaagaaa taccacctgg atagctataa atcatttcgt
    23281 aaatacgaaa gacttaacaa attaatcagc tacgataatc gttccttctt cagaagctaa
    23341 aagattctcg atattttcta aagaagtgat gattgcctta gcgtttggtt ttgcttcaac
    23401 aaatgcaata gctgcttcta ctttcggaag catgcttcct ggagcgaatt gcttttcatc
    23461 aatgtattgt ttcatttctg aaacagtaac agtttctaat ttcttttgat ctggtttttg
    23521 gtaattgaca tacacattgt ctactcctgt caatacgatc agcaaatccg catcgatgat
    23581 ttcggctaat ttttctgaag caaaatcttt atcgattacg gcttctcgtc cttctaatcc
    23641 agtagctgtt tctacaacag gaatcccacc gccaccaaca gagactgtga taactccttg
    23701 atcgaccagc gtttcgatga cacgtgcttc tttaatcgag atgggtttag gggaagcgac
    23761 tacttttctc catccacgtc cagcatcttc tttgaatgtc acagtggagt cagcattcat
    23821 ttgttctttg gcttcttctt ccgtgtagaa tggaccgact ggtttacttg gatttttgaa
    23881 tgaaggatca ttttcatcca caataacttg agtgactaaa gaaaccacat ctttatcgat
    23941 ccctttttcc ttcagtactt cgcccatggc gttttgtaac cagtaaccga tagatccttc
    24001 tgtcattgca acacatgtat ctaaaggcat tgccggtgtt ttctcagaat cagcagcttg
    24061 ctgctgaatc aataggttac cgacttgcgg accatttccg tgggagatga tcaattcatc
    24121 tccttgttcg ataaatttca ctaaatactt tgctgtttcc attaacgctt cttgttgggc
    24181 ttttgcactt gcatccgtag acaagatggc attgccccct aaagcgacaa ctacttttcg
    24241 tttggccatt tcattcgtcc tttcttcagt tctaccgaat tttctagata acaaaacgag
    24301 ccagcgtttt gtgattttcc ctcaatgctg actcgttttt tcgtgagatg aatgtggtta
    24361 tgcttaaacg cgagggataa acaagttacc taatgttgct gccatgatcg ctttgattga
    24421 gtgcatacga ttttctgctt ggtcaaattg acgagcatat ttgctgcgga agacttcatc
    24481 tgttacttcc atttcagtga tgccaaagcg ttctttcatt tgttcaccgt aaacagtgtt
    24541 tgtatcatgg aaagctggca agcagtgcaa gaagatcaaa cgatctgtgt tgtgtgtttt
    24601 ttctaccatt tccatattga tttggtaagg tttcaacaat ttgatacgtt cttcgaattt
    24661 atcttcttca cccattgata cccatacatc agaataaagg acgtctgctc cgtctacacc
    24721 tttatcaaca tcgtcggtaa tcatcaattg tgcaccggat ttttcagcaa agccttctgc
    24781 cattttgacg atttcttctt caggttgtaa ttcttttggt gcaacgatac gcatattcgc
    24841 tcctaaaatt gcacctgtga ctaataaaga gttagccata ttgtttcgac catctccgca
    24901 gtaagcaaca gtgatacctt ctactgttcc aaagttttct tggatagtta aaaagtcagc
    24961 gatcatttgt gttggatgcc attcatcagt caagccattc caaactggaa caccagagaa
    25021 ttctgctagt tcctctacca ttttttgact gaatccacgg aattcgattc cgtcaaacat
    25081 acgtcctagt actttagctg tatcttctgt ggattctttt ttacctaatt ggatatcatt
    25141 tgcacctaga tattcaggat gtgcacctag atcgatcgct gcagtagtaa atgcagaacg
    25201 agtacgtgta gatgtttttt caaataaaag cgcaatattt tttccttcta gatagtgatg
    25261 tgggatacca cgttttttca agtctttcaa gtgttctgaa aaatcgatca aatattgtaa
    25321 ttcttctttt gtaaagtctt tttctgctaa taagcttctt ccttggaata ctgattcttt
    25381 catattaaac attctccttt agattgtttt tatttaagat taaagaggaa atcccgacgg
    25441 cagctgacag taagactgcc gtcaggaaac aaattacttc aagtcttcac ggacaagtgg
    25501 ttggctcatg cagcgtggac caccacgacc tcgtgacaat tcacttgaat tgatttctaa
    25561 cactttgatg ccatagctac gaagcaattc gttcgataca taatttcggt tataagtgac
    25621 tacaacacct ggagcgattg ccaatgtgtt tgatccatca ttccattgtt ctcgaggtgc
    25681 aacgatctca tctccattac cagttgggat cagtacaaga tcatctaatc caagagcagc
    25741 tttcatcgtt tcgtgtaagt tatctgaatg agtaatcttg atatcatcac catctggttc
    25801 gattgtaaag acatccatct taccgtcttt actttgaatt gctggatgga tagtgaattt
    25861 atcgtgatct accattgtaa atacggtatc caaatgcatc attgcacggt tgttcggaat
    25921 tttgattgcc aacacttttt caaatcctga attttttgcg aataggtggc gtgctaattt
    25981 ttctaaagct ttcgcattag ttcgttgaga aatcccaaca gctactactt tatcgcttaa
    26041 gatcaattcg tctccgcctt cgatatgatc tggattttca cgatccaacc atacttcgat
    26101 tcctttattt gcaaatcttg gatgatgttt caaaatatat tctgtaaaca tcgattcacg
    26161 acgacgtgct tcaaaagtca tcttattgac tgtcatccca tttcccattg atgcggaagg
    26221 gtcgcgtgta aagtacaagt ttggcattgg gtccatgtaa aatggatagt catcgtctgc
    26281 tgacagatca tatagactgc tagaacgtac ttcgatatct tttgtacgaa ccccagccat
    26341 gattttatct accatttctt gcgtttccat tgatagaagg aactcatgaa gaccctctct
    26401 tacagcattc gatgcaatat gagattcatc caacatttta ttcaagaatt gttctttgac
    26461 attgcctgcg tcaatcgctt ctgccgctaa tttttccaag taaagagtct cgacgccttc
    26521 attttgcaat gtcttcgcaa agttgtcatg ttcttcttga gcaatcggaa gatatggaat
    26581 atcatcgaac aacagacgat ccataatatc tggtgtcaaa ttttccactt cttgtccagg
    26641 ccgtttcagt aaaactgtct ttaatttccc tatttcagag aaaacgtgaa taggtttatc
    26701 catgttgtgt tgcctccttc tttttgatta catgtatagg atatcctcct tttgtaatca
    26761 ctttcataat tttgtctcat tcaaaatgag acattcctca cattaatatt cgataattaa
    26821 ataattaaac gtttttctat ttttaagcgt aatttattca ttttttattc tgtatacact
    26881 ttcaaaaaaa tatttattca ttgaaaaaat ttttttgaat aaattaaaaa aataaattat
    26941 attatcgttg ttagcgttta ctatatgtat tattgttgtt ttttatacac aaatcattcg
    27001 cttcaaaaca aattattcaa taccattgta tattttttac ataataacaa attatatttg
    27061 taaagtatta aaacttgttt ctaatgttgg tatgttaaaa ctttacaaat tctttaaaaa
    27121 aagtgacaat aaatatagtg aacaataagg agaggacttt ttaatgcgtg ttaataaaat
    27181 cgattttcac ttttcaaaac tcagatatca acctgatttt gaacatttct cggatgaaga
    27241 gttccaacag atcaaggaac atactgttct tcgttcttat aaaaaaggac agatcctttt
    27301 cgacgatggt gatcccagac aacggttgta tatcttgacc aaaggactcg ttcgtttaga
    27361 acgttatgat gaaagtgcaa cttattacta ctatgactat gtgaatacaa ataacctttt
    27421 tcctatgtca ggaatgtttg gagaagagac ctacacttat acagctcaag cgatgactga
    27481 tattgaaaca ttctaccttc ctgtttccta ctatgaaaaa ttagcacgaa aaaatcccga
    27541 acagttgatc tatatgatac aaaaaatttc taaagtagtt gaaatacatg aattgcgtat
    27601 ccaaatcgga ttgacctcta gcgcctttga ccgggtaaaa caaaatttat tcattttgaa
    27661 agaagaattg ggcgtaccaa caaaagatgg tcatatcacc attccttatc cgatcacctt
    27721 aaaagaatta gcagtgaata gcggcacgac acgtgaaact gctggacaag taatcaaaca
    27781 actacgtgat tccgggctgt tagattatga caaaaagcgt tttactttct gtaagacagc
    27841 tactactcca gctatgagag aataacgcga acaaaaataa gcagtgaccc tgataaatat
    27901 cagggtcact gcttattttc ctccctaaaa cttctgaaag atccggcagt tttagatcac
    27961 tggacaagac tcaaagattt tctatgtgtg tttcagttat gtaatattca ctgtttgcaa
    28021 aaaatactgt tagattaact agatgaagcg tcttgtcctc atccgatcta gattattcga
    28081 tattcctcga caataaacga ttactcagtg agggcaatca gaggaacgga taatgataaa
    28141 taagctgtaa aaaatagcaa aataacaata atgattaaaa gtatgtaaaa aatagggctt
    28201 tgacaaaaac gtgtcacagc cctatttcga ttaaacggtg tgtacctgtc aactcttcgg
    28261 tcacaagtca taatgttcat aaacatgagg agctgttttt tgaaattgtt ccttgtgaat
    28321 cagagttaaa cgacaggctt acgaactctt gattaaacgg cgttcaaaag cttgcatacg
    28381 cggtaataag aacagccagg tcaaaaacat gagaagccgt ttttcctggg ctgttcttat
    28441 tacttgatgc agggcagctt tttcacaacc tctttattca gataaaacca agcttggatc
    28501 agcattcaac tgatagctag caaaatttct tttttgcatt tccacatgtg ctgctgctcc
    28561 aatcattgca gcattatctc cacaaagtcg aagaggggga atcactagtt ctacctcagg
    28621 gagtttagct gccaaagcat cctgcaatcc ttcacgcaac ccttgattgg ccgctacacc
    28681 tccagctata atcagttgct tgactggata attttgacaa gctcgaagtg ttttagtaat
    28741 caatacatcg atcacacttg cttgaaagct agctgctaaa tcatttttgt ctagatcttc
    28801 tccacgctgt tgtgcattat gcaccaaatt gataaatgca ctttttaatc cgctaaaact
    28861 gaaatcataa ttatcttcat ggatcatcgc tcgcggaaaa tggtaattat cttttccctg
    28921 atgagctagc tgatcgattt cttttccgct tgggtaagaa agacctaata ctcttccaac
    28981 tttgtcataa gcttctcctg ctgcgtcatc tcttgtctcc ccaatgattt catatgatcc
    29041 atcttcttgc atatagacta attctgtatg tcctccgcta acaagaagtg ccatcaatgg
    29101 aaactggaat ggtttaacca gacgggctgc atagatatgc cctgccatat ggtttacagg
    29161 aataagcggc aattggtgtg cccaagcaaa agccttagct gcagaaatac caatcagcaa
    29221 cgagccgaca agtcctggtc cataagtgac agcgactgcc gataaatctt ctgccgacac
    29281 accagcttct accagtgcat cttccaagca taaggtgatt tgttctacgt gatggcggct
    29341 agctacttca ggtactacgc caccaaaacg tttatgactg tttatttgag aagcaacgat
    29401 gttagataag atctctgttc catttctaac gaccgctaca ctcgtttcat cacaacttga
    29461 ttcaatcgcc agaatcaatt cttcattcat ttcatcgtct ccgtttttaa ctttctgctc
    29521 ataatgatcg catcttccgt cggattgcga taatatgatt tccgtttacc taatatacga
    29581 aacttttctg attcataaag ttttcttgct gcttcattag atagacgtac ttctaaaaaa
    29641 acatgcagcg aatcttttat ttgttccgta atcagtaatt gtaacaaatg ccgtgcatgc
    29701 ccttttcctt gttctgaaac tgcaacagca atatttgtga tctcgacttc atctattact
    29761 ttcgagtatc caataaagcc acagatctgg ttatcttcag ctaaaagcag ataatcagag
    29821 tgaggctgtt ggatgtctgc caaaaactgc attttcgtcc aaggtgaacc atgtggataa
    29881 gcattttcgc taatcatcca tacgcattct gccaagtacg cttcatcaaa atcttttttt
    29941 ctgcaaatca actaatctca ctcattttct aaaagtttta tcatatccaa cgcatcttct
    30001 tttgtttcag tgtaatatct ttttttgatc gttgttgctt gaaaccccaa tttccgataa
    30061 agtctttgtg catcttgatt actcatacgc acttctaatg acatcgtctc acagcgattc
    30121 attatagcaa agttctcgat ttcgtcaata agcagcgaac cgattcgttt acgctgatat
    30181 gccggtcgaa cggcaatatt cgtgatatgg caatctaaac cgaacgctct tgaaccgata
    30241 aaaccaatca ttcttccatt atcaaagata cctagatata gatgaggaag cggtgacttc
    30301 aattctgata taaaggcact ttttgtccat ggcaattcgc cattatagac ttctcgttcg
    30361 acatccagca gctctttgat atcttctgta gtcaattctc ttagtacgta acgacgatcg
    30421 atttgttcga ctgttttttc tgtataaatc attttatctt gaaaaaagct tctggcatac
    30481 gctctaaatt ttttcaacat aactttcgtc tccaggtgtg tgcgtctcca accatttttc
    30541 ttctgcttct actcgtttta aatagcgagg aagaaaagct tgtttctctg caactggttc
    30601 aactaaagca cctaattgag ccacagttac accacttgga atatcccatg ctggaactgt
    30661 attaatcatc gcactaggca atatttcctt tatttcagag aagaatttgt gacaatcact
    30721 cccgacgaaa taaatagctt cattcagctt ttgtagttgt gtgaacaaat cagaagcaga
    30781 aatatgtaca tcttcaagta cagtttctaa tctattttct ttccagcggt atgccccagc
    30841 gtatacattt tttcttcgag catcaaacat gggcacaata atattatcca caccgatgca
    30901 attagccgcg atggttttca gactagatac tcctactagc tctttatcta atgtataagc
    30961 aatcgttttt gctgtcgtta cacctatacg aagccctgta taagaaccgg gaccatcaga
    31021 aaccgcgatt cggtcaatgt cttttggact tatttgaaga tctgcaaaca attggtcgat
    31081 tgcaggcatc agtgttacac tgtggttgcg tttgatattt gtttgtattt gtccaagaat
    31141 ttgttcgtct tctaccacac cgatcgccaa agtttggttt gcagtatcta ttcctaatgt
    31201 aatcatatat ttgatccttt ctgctatgac attctcgcct tccattgtag catacttttt
    31261 tattctgagg atgaaaatac caaaggcaaa actagtacaa taatacatga ttatctgctg
    31321 gcgggcaaat tttgcccctt cctgaaacac aaaaaagctg cggtatcact ccattttccc
    31381 acagcttttc catcttttat ttcgttgatt ctcgaataat caactctgtt tccacaataa
    31441 tctttcttga aaccggtgct ttggaattac ttaattgaag aatcgtctcc actgctagct
    31501 ctcccatcca ttcggtataa acttttacag tagaaagcga gggcgttaca tatttcgcta
    31561 cactgacatc attgaagccc atcacagaaa tatcttctgg taccactaaa cctttttctt
    31621 gaatcgcttt cagtgcgcca atcgctaatg catcattagc tgcaaaaagc gcatcaggaa
    31681 catgcggatt cgtttttaaa tattgcttca ctgcttgata tcctgactcg acagaaaaat
    31741 ctgcttcgat aattggacct tcgtcgtagc ctttttctgc caaaacttgt tgaaatgcca
    31801 taattctggg atcttttagc cgctgatggt ttttcttcgt atattctgtt ccagcaagca
    31861 tagcgatatg ttgcgcttta tgggagacaa agtgttcaat gactttttga acacttgact
    31921 taaagtcgac aactagagag tcgattcctt gagaagtgcc gtcagaatct accaaaagca
    31981 aggcattttg ggatcctttc atttctaata actgagcttc gtcgaatttt cctagtgcga
    32041 tcgttccatc tgccggaaga tcactaatgg aatcccacgt ttctttcaat aaagtaacgt
    32101 ttgcttcttc tgcctttttt tcgattccta ggcgaataga taaataataa agatcttcaa
    32161 gctcttccgt atcgtcgtac cactggatca ggcgaagcgt ttgcttagta tttgctgcat
    32221 ttttacgatg tttcgtataa tttaagcgtt ctgctgcttc aaaaattttt tgctttgtgg
    32281 ctaagccaac ggaaagatca ggatcatagt tgagaactct tgaaacagta gcaggagaaa
    32341 ctcctgccaa ctgcgcaatg tctttaattg ttgccatttg tctctcctcc ttttttacgt
    32401 ttataaaatc gcagtaaatc gttcaaacgc tgcctgtcct ttttcatctc gcttgaatac
    32461 tccagcgtct tccagtacac gtgcaaaaac ctgacctaca gatttttgta cgacatcggt
    32521 gaccgtatct ttcgtcaaat caggatgttc tttttttaat tgttctgccc acggacgatg
    32581 atattcagcc atctggtttt cttctcctaa taagaaatgc tctacttcta ctagttctgg
    32641 tttcaaacga ggtggtaaaa cagcaagccc cattacttcg atcaacccaa tgttctcctt
    32701 tttgatatgt tggacatctt tatgtggatg aaaaatccca tcaggatgtt cttctgaaac
    32761 attgttgtct cgaaggacaa ggtccaactc aaagtaattg tcccttcttc gtgcaattgg
    32821 cgtaatcgta tggtgtggtg taccatctgc tgaaaaagct ctgatagata ctgtttcatc
    32881 cgaatatgct ttccattttt ccaatactga gttagctgct tcaataagca agtttttctc
    32941 tgttgattgc aaacggatca cagacatcgg ccatttcacg actcctgcga tgacttgcgg
    33001 ataatcttct agtttgataa gtttttctat cggtgctttt gccataggaa attcatgacg
    33061 tcctgcctga taatgatcat gagaaagaat cgatcctcca acaattggta agtcagcatt
    33121 cgaaccaaca aagtaatgag gcaagacttc agtaatccga agcagtctag caaatgtttc
    33181 ctttgtgatt ttcatcgggc ggtgttgttc tgataaaatg atcgaatgtt cattgtaata
    33241 agcataaggg gaatattgga atccccagct ttcaccgtct agattcattc gaataatccg
    33301 gtgattggtg cgggctggat agttcaagcg acctctatat ccttcgtttt ccatgcaaag
    33361 catgcaggta ggatagtcta cttttaccgc ctcgcgttct gctgcgattt gttttggatc
    33421 tttttctggc ttagataaat tgattgtgat ttccagttca ccatactccg actctgccgg
    33481 aaatttgatg ttttttgcga tggctcttgt tttgatgtag tcatttttct tacttaaatt
    33541 aaagaaatac tcagttgctt cctctgggct tttcgaatag tgctgtgcaa agaatgcatt
    33601 gacgactgaa ggtggcggtg tgagaaaatc cattaattgc gcttcaaaaa tctcttgttc
    33661 tgctggcaat tctgaaagaa caccattttc tcttgcctta gcgacaagct gatccaataa
    33721 gtctacagat tcttccttaa ctggtcttac ttcaatgctt tctagagcat cttctcctac
    33781 catccctaaa agacgattct gtaaatataa gcggtccatt tccatccatc cgccagcttc
    33841 gatcgcaagt gtggcaaaat cacaaatcgt ttgactgatt gacatatctc attcccctat
    33901 tcttcataac catttggatg gctgacatgc caatcccatg ctgttttgat aatcgctttg
    33961 atatctgtgt attcaggttt ccagcccaga atctctctcg ctttttcact tgaagcgact
    34021 aatcggctag gatctccagc tcttcttggt gctatttctg ctggaatttc ttttcctgtc
    34081 acttctctag ctgcttctag catttccttg actgagtatc ctttgctgct gcctaaattg
    34141 aagaagttgc tttcatttcc ttctttcaag tattccaaag ctaaaatatg tgcagcaatc
    34201 aaatcttcta cttgaacata atctctgata catgttccat ctggtgtatc ataatcgtct
    34261 ccataaacag ccaaggcttt tctttgacca agagctactt gtaaaatgat tgggacaaga
    34321 tgcgtttctg gtgtatgatc ttctccgata gatgcatctg cttttgctcc ggctacattg
    34381 aagtagcgca aagcgacata gcgcatgcca tatgcttggt cacaccactt catcattttt
    34441 tccatcatca atttactttc accatacgga tttttaggat tagctggtgt atcttctgtg
    34501 attggagact ctttcggctc tccataagtt gctgcagtag aagagaatac gattttattc
    34561 acgttgaact cgtgcatgac ttcaagtaag atttgcatac cgtatacgtt gttgttgaaa
    34621 tacatcaatg gtttttcgac agattctcct actaaagagc ttgcagcaaa atggattact
    34681 ccttcaatcg gttccttttc aaatacggaa cgtagaaatt ctttatcccg gatgtctcct
    34741 tcataaaaat gagcatctgg atgtactgct tgtttgtgac ctgtgagcaa attatctacc
    34801 acagctactt gatacccctt ttgaactagc tggtctacag cgtgcgaacc gatataaccg
    34861 gctccgccta gtactaaaat tgtcataaaa aacactcctt tgattagtaa aattgtgttt
    34921 gttgattcta tataatttaa aaaacgaagt ttacttttat ggtgaaaaca aacaaagtaa
    34981 aatttcgtaa attcatatgg taaatttata ataaaacatt tagtaaacat tttcaagggt
    35041 tttttgcaaa ggtttacaaa caaacgtaaa actagccttt ttttctcttt tgttccataa
    35101 tagatataac aaataataaa tgaggtggat tcaatgaaaa aatttgtatc tggaatatta
    35161 gtaggaagtc tggcaacagc cgctgccgtt gctggtttgg ttgcttctgt gaaaaaaaca
    35221 gtgatcgatc caattgacga aaaagaagca atgatcgaag aaaaccgtaa aaaagcaatg
    35281 cgtaaacgcg tttctcgtta ataattattt aaaaatacgc atgaagactt tctaaaaaac
    35341 agataagtca tcatgcgtat ttttatttgt cagtcaatat aagttgtgct attttatctg
    35401 cctgtctcag attccatgga tctgtataag gtctagctac tgctgcagtt agccaatcca
    35461 caccttcaac aggtattcca tagcaataaa agtcagactg gatttcccct gaagcatcaa
    35521 tcacgcgatt cgtacctcgc tcaacatcta tagcttctga atgataactt cctgtattat
    35581 ctgaaaatga aaaagggcga acaagtttgt tttccttcaa attttttatg acaggattac
    35641 ttgaacgaga aaagcttgtt tgtggcaatc gagcttcaat caaatagcga ctgctgattt
    35701 ctttatcttg tagagaaata acatagcggt tgtgcttttg ctgaatatca aatggcggtt
    35761 cgatcaaatg aaaaataccc gcatctatca gtgctgctaa ttctttcgtc cgaatgattg
    35821 gcggaccgat cgttaaaaag ctatttagat gagtaaacca tccccatagt atttctttgt
    35881 attccttagc agaaaaaacg tgccattcaa tagcctgccg gattggatca cgccagtctt
    35941 ttaatgcatc aaatgttgaa gcaaatgctc ctgtgcaatt gcctttttcc gcttctttta
    36001 tctgcccttc aataaatatt cggctagctt ctgaaaaggg ctcattctga taggcaagag
    36061 gagtttctaa atacgaccaa tcccatctat aagggattag tgctggatgc tttccgagac
    36121 actcctctga agagagggag agaaaatcat gctcaaaatt tttctttgga atcggcaagt
    36181 gatattcttc gatgattttc ttgtaataaa acaactcaac gtcttttttc agatagtcaa
    36241 aaaatgtttc gccagtcaaa aatcccgact gatgccattg ctccaggttt tcttttgtta
    36301 gtaaccgtgg ccatgccatt gctccgcctt gcttttgatt tcttcctcga gggtaataag
    36361 ggagccccct tcttgatcca ctgtatacaa tcggttcctt gcctgatgga tgatagacca
    36421 gttttccctt ttcgttctcg aagcgccctc ctcttgatac tgtaaacaat ccgacataat
    36481 caaaaaaact taagcctaga cctcttaaaa agacattttc tttaggcggt atcttcgaaa
    36541 gatctacatc tgcaggatta gaaggaagct gatacagtaa aacatttttc tttgcgtagt
    36601 ctacccacac tctttcttct tctgtctcat cattttccca gtgaccgctt gctaaaacaa
    36661 gctgatcgat agaatagctt tgctgatctg tctgtaaaag ataaccatct tttcttttgc
    36721 ttactttttt tactaaagaa taaacaaact gaatagaagc tggaaattct tcttgcaact
    36781 ttgagaaaaa ccagcgttga tagagtccgt ataaacatcg agtagattgc tgatcttttt
    36841 ccagacgatt gatttcttct ataattagtt gcttattttt ggttaccgtc tgttcgatat
    36901 aatctgtagc aggtccttta ctccattgaa acaagttggg tccctgtgtt accaatcctc
    36961 cactggacaa tgtttcatct gtaaaaagcg tcacttgttg agaaacagaa ttcattaata
    37021 actccggtgg ctgattcaac cgccaaactc gacctccagg accattggga tcgaaaagaa
    37081 aaagttcaag agaatgtctg tcctgattat tatggagaat cctttctgct acacttaatc
    37141 ctctaggtcc ggcaccaatg atccctattt tcatttgcat gcctccattt cgataaattt
    37201 tttcaataaa acagcatggt tctgatgttc attttttgca ccataaacaa aagtaatgac
    37261 tggatgttct ttcataagtt gactgagttt aaaaatagct tgcttctttt cttcttcgaa
    37321 aatcaattct tgctggtatt tctttgcaaa tgaagcaaat cgttctggta catggtcaaa
    37381 agttttgcgt gcttcattac taggtgcaat ttctttcaac cacagatcta actgcgcttt
    37441 ttcttttgaa attcctcgtg gccagaatct atccacgagt attctatagc catcctcttt
    37501 ttcagcatca aggtaaacac gttttaattt gatcatagtt gtttccttct ttctaccttt
    37561 tcgcttagct tatatatgta gataatagca caaaataaaa aaaatgaacg gcacaactat
    37621 ccattgcgcc gttcatgtga tcttggtcaa aatctaatga ttgattcggt ttttaaagct
    37681 gtcatacaat catctgtttt atactttttt acttttgatt tttccagacc atccgtcata
    37741 tccaccctta agtaaataaa tctcttgata acctgcttta cgtaatttat ttgctgtacg
    37801 gatgctcaaa cttttctttt gatcgtaaat atagacaggc tggtcttttc gaatagatcc
    37861 taatgaatct ttcaaaacag tgtaagggat gtttcttgca cctaaaatat gcccagcatc
    37921 aaacacatct ttttcgcgaa catcgattac ttgtgctttt ctcattcctt ctcgaaattc
    37981 ttcttctgtc aaagtcgttg ctgaacgttt ggccattact cgtagataga cttcccaaag
    38041 gatgattgca agcacaatca atagcaaaat gatattaatt acccataaaa ccatgtaatg
    38101 cctccgtttt tttaagcaaa tttcaaggct aatgaggcag caccaatgac accagcttca
    38161 ttaccaagct ctgctaattt gatttttgtt gattctctta cttgcgggaa agtaaattct
    38221 ttaaaatatt tctctacacg gctacgtaaa aattctcctg ctgccgatac tcctccacct
    38281 aacacgatac ttgacggatt taatgtattc cccaagtttc cgcaagctaa acctaagtag
    38341 aagcatacgc gatcaacaac catcaatgca aacggatcgt cttcttgagc catttcaaag
    38401 atatctttac ttgttacttc ttctccgttg tctagcattg ctttcagttt tgaatcccct
    38461 gcgtattctt ctgccaagaa acgacctagg cgtaccacac ctgtagcgct agatacagtt
    38521 tctaaacatc cgcgtttacc gcaagtacat tcgaaccctt caggatccac agtgatatgt
    38581 ccgatttctc ctccagctcc gctcacgccg tgaatcaaat ttccgccagc aacgatacct
    38641 ccgccaacac ctgttcctag agtaacgaat acaacgtctg gatcgttttc tccagcacct
    38701 ttccatcgtt cacctaaagc agcaacattc gcgtcattat cgatagcgaa tttgattcct
    38761 gttcctgcct cgatcagttt tttcacaaat tgaacttctg tccagtttaa gttatacgca
    38821 ccaataaccg ttccagcttc gctgtccacg cttcctgggg tacccatgcc gattccgata
    38881 aagtcttcgg ctttcatatt atataaattc aaacgatgat tgattgattc aatgatttca
    38941 ggaacgatat gcttaccatc atccaaaata ttggtatcga tgctccattt ttgctgaatc
    39001 tctccttctg gtgttaaaat tgcaaatttt gctgttgttc cacctaaatc aatcccaata
    39061 atcttcttgt ccattacaaa tttcctttct ttcggctttc ttcaattcgg tgctctcttt
    39121 ttaatatgcg ccacgctgtc atgtatgtat catgatcaat caaacggcta tcatacagat
    39181 tacgcaattc gatagtcatt aattcaatgt catagatgcg tgcacccaca tagataaaga
    39241 tgccaaattt tttgaacagc tgctgtacat catataagct ctccatcttt aagcacttcc
    39301 ttcatattat acaagtaaac catatttttt taaacccagt aaaatacata ttacaaaaag
    39361 aaatacaaaa actagtgtag aaagaatcct ttggtgtatc gaatagcttg tctgttgttt
    39421 cggtagaccc aaaacattcc ctaacactaa tccgccaatt agcccaccga catgtcccca
    39481 aacatctaca gaggtatcca gaagaccaaa aacaaatgtc aacacaataa ataaaagaaa
    39541 atgtctgact agctgctgga tcgctgggtt atgtttaaaa tgaaacccta agataaacaa
    39601 cgcaccgaac aaaccgaata tcgaagtact tgcaccacca gacagaacat tcgcttcgtt
    39661 aaaagcaaag cttgctgtat ttcccattat cccgctgaac atataaatca agaaaaaacg
    39721 ccaatggcca tagattgcct cgatttgctg ccccataaaa tacagtatca gtgagttcat
    39781 tacgaaatgc atcagtccaa aatggatgaa gattggtgtc actaaacgcc accattcatt
    39841 gaagtgaaca agataaggac catacattcc ggcataataa gggatctcaa gtcgcggaaa
    39901 taattccatc atgaggaaga ccgctgtctg tatccctaaa aaagcatagg tccataacgg
    39961 tcgtcttaac catgcacgca attttatttg ctgttgataa ttcatgaaaa cctcccattt
    40021 cattttagtc aataaacaat tgttggatct tctgatcaaa gctctccggt tgccattgtt
    40081 cattcaattg ttcacgaaaa acaaggctac atgttttttt atgaaaatta tttaaatacc
    40141 ggtcgtaata accaccgcca aaaccgattc gataaccggc tctattgtag ataatacctg
    40201 gtacaatgag gagatcgatc atctccggca aatagagctt ctctgattgt ggctcaagaa
    40261 caccaaaact cgaacgtaca agaacggact gttcatccat ttcataaaac gccatctgac
    40321 gtttcggcaa agttttaggt acaactattt tcttttgttc tttttctgct cgttggataa
    40381 ccggctgtgt atccagttca aattcattcg ataaagtcgt ccctatcact tttgctgact
    40441 tccagtactc gctggcgaat agctgtttat agattcgctg ttctttttcc tttttcttgc
    40501 cacttttggc cagatttttc agcttttcga ttcctttccg gcgtaatctt actttcaatt
    40561 ggtccaaaaa ctcacccttc cttctctcat aaaatggtat aggaaaaaaa gaaaaatagc
    40621 aatcgaactt tcaaataaat gcataaatag aataaaaaga agacctagaa atgattggcc
    40681 ttcctttttt aatgaatttc aactgttcag cgcgtgtttt tgtctgataa aaagtgcata
    40741 cagaaaatat ggcgcaccaa aaagggcaat caaaattcct gtaggtactt ccccacttgg
    40801 tagaatcact ctagcaacca tatctgcact aagcagaaaa atggaaccga acaaaccgct
    40861 cagaacaaag gtccagttat tttctttacg tacgactagc ttcgcagcat gaggagctag
    40921 tagtcctaca aaagataaac tacctgtcac cgctacacta ccgcttgcca aagcaacagc
    40981 taacaacaaa aggatttttt gacttttagc cacgttaagg cctaatcctt gcgcgcgttc
    41041 ttgaccaaat gatagcaata caatcctctg tgcataaagt cctcctagcg gaattaagat
    41101 aaccgcccat ggaagcaatg cccaaacata aggccagctc gcaccccaga ttgatcccgc
    41161 taaccatgca ttcacaaatc cataattttc cttagataat tggatcgtgc atagcaatgt
    41221 taatgcagaa aggccggcat taacggcaat accggacaat aagattcgat tcatacccaa
    41281 aaagccattt cgatcatagg ccgcaagata gacacagcaa gcagctaaaa caccacctag
    41341 aaaggctaca actagcaata gaaacggcag cgaatagtgc gaaaagaaac ctaaataaag
    41401 catcactaaa aatcctgctc cggcattaat gcccaatata ctagaatctg ccaattcatt
    41461 tcttgttact ccttgcaaga tataaccaga aacgccaaga caagctcctg ctaataaact
    41521 gatcagtaat cgaggaactc gaaattctct aacaatcagc gccaaagaag atgattcttt
    41581 ccctatgaac gtggcaaaaa gctgatccag gtaaataaaa ggcgtaccaa tcattaaact
    41641 acctatagac aggatgacaa gtacaaaaag caagaaatag aacgtccaat atattttttt
    41701 caccgttgtt ccctccttac ttgtatcagt aaaaacggaa cgccaatcaa tgcaatgact
    41761 aatccaaaag gtgtttcaaa agggggattg atcatcctcg ctgtaaaatc cgccaacata
    41821 accaatccac cgccaactaa aaaagagaca ggtagaacat aacgatagtc cgttccaacc
    41881 attccgcgaa caactggcgg aaccaacagc ccaataaatg aaacaggtcc gactaacgaa
    41941 acggcaatgg ctgctaacac tgcaactaga cagaaaacaa tcaaccgcgt tcgtttagga
    42001 tgacttccaa gagctaatgc atgtgtttca ctcaaatgaa tcaaagatat tgcttttcct
    42061 aatgaaaaag aaaccaaaac agcaagtata tataaaggaa acaatactgc taattgtttc
    42121 catgtgatat tggcagctcc gcctacaaac caaaaagcaa gatcctgctg cagattaaag
    42181 aattgggtaa gtgcttggct gatagcagta aaaaaagaac taatagctac gccagctaaa
    42241 atcagctgta aaggattcat gcccattttc gaaaactgca aaaaaccaaa tacgagcaaa
    42301 gttacagcaa aagaacctgc caatgacatt agaaacacag aaaaatcaga agctttaggc
    42361 caaaaagcaa aactaagggc cattgccaac gaagcacccg cgttgattcc tagtaagcct
    42421 gaatctgcta aaggatttct tgtcactcct tgcatcaatg caccgctcaa tgcaaaacaa
    42481 ctccctacaa gaaatgctcc cgccacacga ggcaaacgaa tatttcgtat gatttggtga
    42541 ctttgttttg atctatcaaa atcaaacaac gcctgccaaa aatctgcttt ttccacagtc
    42601 gctgcgccat ttgaaagagc aacaaaaaaa ataatcaaaa gaacaaaaca gaccaaaagc
    42661 aggagtctat ttttcttcat agtcctcata attgatcctc ctgctctttt ggcaacaaat
    42721 cataggttag aaaaactggt ttatttgttc ttggaagcgt gacaatcgtt gcatcaatct
    42781 caaatatttt tttcatacac tcttttgtga tcacttcttc aggtgtacct tctgtgatca
    42841 acttaccgtt tttcattgct aataaataat ctgaaaaacg tgaagcatga ttaatgtcat
    42901 gaatcgacat caagatcgtt gtttgtttca tttgattgat ttcagtcaat aactgtagga
    42961 tctctaattg gtgtgccgga tctaaaaatg ttgtcggttc atccaagatc aagatatctg
    43021 tttcctgagc aagcgccata gcgatccaga cacgctgggt ttgtccacca gacagcgttg
    43081 cgatatcccg catacgaaat ccagctagac ctgtagcatc caacgcccac tcaattgctt
    43141 catgatcttg cgtgtccatg ccactgaaca gtttttgata agggtatcgt ccgtaagata
    43201 ctacttcttc tactgtcata cctgtagggt gttcactagt ttgtgctaat aatgcaattt
    43261 tttttgcaag ttcttttgaa ctgtatgtag agatatcaga gttattgaca atgatctttc
    43321 ctgatttcac aggtagtata cgggtaaaac ttttcaataa agtagatttt ccacttccat
    43381 ttggcccaat caaactagtg attttccctt caggaatcgt aacagacatg ttttcaatga
    43441 tttttttgtt ttcgtagccg gctgcaatgt cagctgctct taatctcgtc atttcaccca
    43501 tcctttcgct gaaaatcatt ctcaattatg acgctgtttc aacatttcgt caagcgctac
    43561 tttcatatct atagccaaat gaagcggttg acagcgttcg aaatatcagg aataataacc
    43621 aatgagaaag attctcaatt atgttaggag gaaagaaatg aaattaaaaa caaaagtatt
    43681 cacattatct agcatattgc tgctggctgg agtactgagc gcttgctccc cctcttcaga
    43741 taaagaaaca gctacaactg ccaattcgac gacttcatcc aatagtactc atacagtcac
    43801 agatacactt ggacataaag tagagattcc aaacgcgccc aaacggatca tcggcagtta
    43861 cttagaagac tatctgattg cactagggga aaaacctatc gcccagtgga ctgttggcag
    43921 tggttccatt caagattatc tgcaagatga tttaaaggat gtcccaacga tttcttatga
    43981 ccttccttat gagaaagtgt taagctttga gcctgatttg ctgttgattt cttcttccgc
    44041 tactgtcgaa ggcgggaaat acgaacaata tagcaaaatc gcgccaactt atgttgtaaa
    44101 aaacggaaca gatgtcacat gggaagaaca actaaaagat gtcggcaaag ctttggcaaa
    44161 agaaaaagag gcagaaaaaa tcatttccga ttatcaaaaa tccatcaaga aaactcgaga
    44221 agaactagca gataaaatcg agaacaagac agctgcagtg ttgtgggtga ccaacaactc
    44281 agcctttatg gtcagcaaaa atcggtcaag tggaagaatc gtatacgacg atctggaatt
    44341 tggtgttcct gatctagtgg aagaggtctc caaaaaagcc acttctgact ggtctgctgt
    44401 ttcctctgaa aagctagctg aattagatgc tgactacatc atccttgtga acagtgacaa
    44461 agatgctgcc atgttcaatg aaccaagctg gaagaatcta aaagcagtga cagacaatca
    44521 tgtactgcaa ttcggacctg aatctagttg gctatataac ggacctattg ccagtcagga
    44581 aatggtcaat gatatcaaag aaaaactaaa ttgattttaa ctaaaaaaag catcttcacg
    44641 attagtcgtg agatgctttt tttcttatta acaaactaga cgcatagatt ttattcttct
    44701 tttgaagcaa ccctattttt tactctttcc catttttgaa aacgatgggc atatagattt
    44761 tcttgatccg ttatcccctc atactcctct ataagggcga agtctgaaaa atcaatattc
    44821 ccaatatacg tatcgccttc aaacgtgcta tcgatcaatg ttcgccagat ccgaccagtg
    44881 taaggaagaa gttcttgaaa aatccgacta ccacccgaaa catagatatc ttctgagatc
    44941 gtttgactat acgacaaaag ttcatcgatc gaatgcatta cttccgcatt ttctttcgtg
    45001 cgaaaatttg gttgcgttgt caacacgatg atatggcgat aaggaagtga aagatccccc
    45061 attccttcat aggtctttct tcccatcact aaaattcgat ccattgtatg ttcgcgaaag
    45121 aaacgcatat catttggcaa tcgccatggt aacaaaccat ttttgccgat cagaccattt
    45181 ttatcttgtg cccacatgga aataaacata ttatccgctc cttatgattt tagatgtttt
    45241 gagataaaaa aagtctcaat tctttgacca gcaaggaatt gagaacttta aaaacattat
    45301 tttgtttcac ggtgcaaagt aactttgcgt tcgcgtgggc aatatttttg tttttccaaa
    45361 cgatcaggat tgttacgttt gtttttgctt gttagatagt tacgttcttt acaagaagta
    45421 cactctaaag tgatgtttac gcgcatgttt ttccctccca tttttacaag aatcctctaa
    45481 gatgactctc agacttgatt atcttaccat gattttttct agattgctag tgtttttcac
    45541 gattttgtta agtaaaacta cttgacgata aatacaacgg ttttacttct caaacaaaat
    45601 ctttttcttt ttgtataaat acccttacac ttgtttatat agattatgtt ttttaataaa
    45661 gggggatact aatatgccat ggaacatgaa ggactttcct gcatcaatga aaaacttaga
    45721 taaattgaca agaaaaaaag caatcgacat cgcaaatgca ttattagatg aagggtaccc
    45781 tgataaccgt gcgatcccaa ttgccattga tcaagcaaaa aaatggtatg aagatgctag
    45841 tgaatcagaa aaacgaactt tcgaaaaaga aaaaaatcca agcaagacgg atgaacacga
    45901 tacgaatcct cgtgctggaa aactgcttga ttcagatgtg atcgtagaat atgaggaaga
    45961 acaatggatc gtgaaatcta aaggggcaaa aaaggcaagc aaccattttg atacaaaaaa
    46021 agaagcaatc gaaaaaggaa agcaagtagc acaaaacaaa gaatcaactt tgttcattta
    46081 taaaaaagat gggacaaaag aaaaagaaat cgattattga acatatgtaa aggggttgtg
    46141 acgaaaatcg tgtcacaacc cccttatgtt cgaaacaatg gtgttcctct gccaactccg
    46201 aaccataagt cataatatct ataaaacggg aagagctgtt ttttgaaatt gtttcttatc
    46261 actcagtgtt aaacggtagg agaaaacctc ttttttattg atatttgatt ttcatcaaac
    46321 agtcacatag tcgattgctt ctgcttgcgg aatcgtttca tctacaaagt agactttata
    46381 ccatgtcaag aaactgaaga ttgtccagat ttttctttgc tcttctttac gtccttcgtg
    46441 atggtcatct agcaacttca tgattttttc ttgatcaaaa aattcttttg caaaatctgc
    46501 ttcgaataat gctttgactt gttcgtagcc gtgttcttct ttcaaccaag ctttgattgg
    46561 cacagggaat cctaatttca cacgattcga ccattcttcc ggaagatgtt tgttcgcagc
    46621 ttgtctgaaa atatcttttg tgttgttttg attcaataaa aacttacttg gtatttgttg
    46681 agccaatttc atgacttcga tatccaataa aggtactcgg acttccaaag aacttgccat
    46741 agaaagttta tctgccttta acaagatatc ttttggcatc cattgatgaa gatcgacata
    46801 ttgcatctta ttgacttcat ctttgattcc ttctgttttc tcgtaatgaa cagtcatgat
    46861 ttcatccaca gatggtgctg tttgatattt tggtgttaac acttctgatg cttctgattc
    46921 ttcgaacact cgagcatgac cgataaaata ttcttttgca ggagcaagtg attcatataa
    46981 atggatccgt ccatggaagt ttttcatttt tccgatttta cgtcctaaat tatagcgtgt
    47041 tccttttggt aattttttca atccttgagc aatcacacgg atgaattttg aattcgtatg
    47101 gaatccatac gtttggtatc ccgcaaacag ttcatccgcc ccttctccag attggactac
    47161 ccgcacactt tgtgaagcaa gatttgctaa aaagtacaaa ggtacgcaag aaggatttga
    47221 atcaggttca tctaagtgat actggatcaa agggaatgct ttgaacgaca tgtctccgtc
    47281 gatcactgct gctgtattat ctaagtccaa tagttcggtt agttttctgg cttcaatcgc
    47341 ttcattatag gttttatcat caaacccgat tgagaaagaa tgatctggtt tcaatacaga
    47401 ggttacataa ctggaatcaa ccccgctgga taagaaagaa ccaacttcta catcactgac
    47461 cgtatgtgct tcaatagaag cttgaacggt ttcgtcgatt ttttcgatcc attcttgtcg
    47521 ggaatgcgtt tcttttgttt caaaatctgc atcccaatat tgctgaatgt ctatttttcc
    47581 atctttatac gtataataat ggccttcagg caaacgatag acatctttga agaatgtttc
    47641 gccgtttaat ggagaatatt ggaaagtcat ataaggtttc aacgcttctt tgttcaattc
    47701 tttgttaaaa tcaggatgag gcagaaaact tttgatttct gaaccaaaca taaatgttcc
    47761 gttcatttct gcataataat atggtttgat cccaaagtgg tctcttgcac cgaataactc
    47821 attcttttca ttatcccaaa tagcaaaagc aaacattccg cggatctttt gcaataattc
    47881 tgttccccat tcttcgtaac catgcaaaag gacttctgta tctgcatgtg tttgaaaaac
    47941 gtggccagct gcaatcaatt cttctctcaa aggctggtaa ttatagattt cgccattaaa
    48001 aataatgatc tttgtccgat cttcattgta aataggctga gtaccgccct ctaagtcaat
    48061 aatacttaat cttctgaatc ctaaagcaac atgtttatcg atatattctc ctgaactatt
    48121 aggtccacgg tgaacgattc gatccatcat tgtattaata attgtttttt tgttgtcctt
    48181 gtcgttaaca aaacctacaa taccgcacat ttaactttca catcctaata taaaattact
    48241 gtttatttca ttcatatgca tcttatcata gaataaatag cggacgaata ttttctctct
    48301 tacaatttcg tcacgtgaaa acaaaagatg aaagtttctg tttttacaga aaataaagtc
    48361 ccaatatcta atttaccatg ttttcttttt tgaggctacc ttattctctt cgtgttaaaa
    48421 aaatattagt ggatgctatt tccaaaaaaa ccagcaaaaa aagacacgac ctaaagatcg
    48481 t
//
LOCUS       DASHHB010000017        47374 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.017, whole genome shotgun sequence.
ACCESSION   DASHHB010000017 DASHHB010000000
VERSION     DASHHB010000017.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 47374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 47374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..47374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.017"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(29..1261)
                     /locus_tag="U2K71_001037"
     CDS             complement(29..1261)
                     /locus_tag="U2K71_001037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEM7315140.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
     gene            1516..2085
                     /locus_tag="U2K71_001038"
     CDS             1516..2085
                     /locus_tag="U2K71_001038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7315141.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            complement(2263..2703)
                     /locus_tag="U2K71_001039"
     CDS             complement(2263..2703)
                     /locus_tag="U2K71_001039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEM7315142.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            2861..3625
                     /gene="map"
                     /locus_tag="U2K71_001040"
     CDS             2861..3625
                     /gene="map"
                     /locus_tag="U2K71_001040"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEM7315143.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            3657..4580
                     /locus_tag="U2K71_001041"
     CDS             3657..4580
                     /locus_tag="U2K71_001041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEM7315144.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(4805..5044)
                     /locus_tag="U2K71_001042"
     CDS             complement(4805..5044)
                     /locus_tag="U2K71_001042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315145.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(5049..5468)
                     /locus_tag="U2K71_001043"
     CDS             complement(5049..5468)
                     /locus_tag="U2K71_001043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM7315146.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            complement(5695..6933)
                     /locus_tag="U2K71_001044"
     CDS             complement(5695..6933)
                     /locus_tag="U2K71_001044"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7315147.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            complement(7040..7675)
                     /locus_tag="U2K71_001045"
     CDS             complement(7040..7675)
                     /locus_tag="U2K71_001045"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7315148.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            complement(8017..8652)
                     /locus_tag="U2K71_001046"
     CDS             complement(8017..8652)
                     /locus_tag="U2K71_001046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315149.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            9164..10306
                     /locus_tag="U2K71_001047"
     CDS             9164..10306
                     /locus_tag="U2K71_001047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEM7315150.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            10299..10505
                     /locus_tag="U2K71_001048"
     CDS             10299..10505
                     /locus_tag="U2K71_001048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEM7315151.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            10584..11777
                     /locus_tag="U2K71_001049"
     CDS             10584..11777
                     /locus_tag="U2K71_001049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM7315152.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            11982..13121
                     /locus_tag="U2K71_001050"
     CDS             11982..13121
                     /locus_tag="U2K71_001050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEM7315153.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            13114..13914
                     /locus_tag="U2K71_001051"
     CDS             13114..13914
                     /locus_tag="U2K71_001051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM7315154.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            13914..14741
                     /locus_tag="U2K71_001052"
     CDS             13914..14741
                     /locus_tag="U2K71_001052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM7315155.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            14719..15453
                     /locus_tag="U2K71_001053"
     CDS             14719..15453
                     /locus_tag="U2K71_001053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM7315156.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            15553..16419
                     /gene="rfbA"
                     /locus_tag="U2K71_001054"
     CDS             15553..16419
                     /gene="rfbA"
                     /locus_tag="U2K71_001054"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEM7315157.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            16433..17005
                     /gene="rfbC"
                     /locus_tag="U2K71_001055"
     CDS             16433..17005
                     /gene="rfbC"
                     /locus_tag="U2K71_001055"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEM7315158.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            17027..18055
                     /gene="rfbB"
                     /locus_tag="U2K71_001056"
     CDS             17027..18055
                     /gene="rfbB"
                     /locus_tag="U2K71_001056"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEM7315159.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            18153..19004
                     /gene="rfbD"
                     /locus_tag="U2K71_001057"
     CDS             18153..19004
                     /gene="rfbD"
                     /locus_tag="U2K71_001057"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEM7315160.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            19038..21071
                     /locus_tag="U2K71_001058"
     CDS             19038..21071
                     /locus_tag="U2K71_001058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315161.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            21115..22395
                     /locus_tag="U2K71_001059"
     CDS             21115..22395
                     /locus_tag="U2K71_001059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEM7315162.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            22605..23411
                     /locus_tag="U2K71_001060"
     CDS             22605..23411
                     /locus_tag="U2K71_001060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315163.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            23423..24643
                     /locus_tag="U2K71_001061"
     CDS             23423..24643
                     /locus_tag="U2K71_001061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315164.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            24633..26219
                     /locus_tag="U2K71_001062"
     CDS             24633..26219
                     /locus_tag="U2K71_001062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM7315165.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            26258..28396
                     /locus_tag="U2K71_001063"
     CDS             26258..28396
                     /locus_tag="U2K71_001063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM7315166.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(28764..29747)
                     /locus_tag="U2K71_001064"
     CDS             complement(28764..29747)
                     /locus_tag="U2K71_001064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HEM7315167.1"
                     /translation="MNKQELHDFIGFRQPNICQIVAYKDGVEVYTDEWNGYKKDDTCH
                     IMSATKSIVSLLIGIALEQGLIKSIDDKVLDYFPEYKVKRGEKTIYAITIKHLLTMKA
                     PYKCKGDPWTKVCSSEDWTKTSLDLLGGRKGITGEFNYQTVCLHILTGLLYQTSKLTT
                     VDFANKYLFDPIGIPNHVNYYAETAEEHKQFTMDKSPKTNVWFCDPLNIGTAGYGLCL
                     SASDLAKIGVLCLNKGMYKGKQIVPPMWINEMFKPRTSALEDLQNMRYGYLWWVLDSE
                     NEIYSAIGNSGNVLYIDAKNNLVISITSYFKPMIIDRVAFVEEYLKPFILK"
     gene            30125..31516
                     /locus_tag="U2K71_001065"
     CDS             30125..31516
                     /locus_tag="U2K71_001065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEM7315168.1"
                     /translation="MNKNGEWSAARRIFIILMDVIVYNAAIYSSFLLKFRGEIPLRNL
                     ETFQHSAIIISVIFIALNILLGAYVFYNRMISDIVFVTVIGQVLMTLGIMVVTFAGRW
                     FAFPRLVILLSFVLGTLFLSIYRILVYKLYLRVSRDKKVVIIGLEKDVAPAIQNFGSK
                     KNNKHKVEAVILDNYYANIKNMIKDIDIVYLASHIEETEKIKIYQLVTKNEKKLFLNT
                     TFENLTLINPNIMNFEDESIIEASGFRIQPEYELFKRLFDIIVSLVLLVIASPFMLFT
                     AILVKVTSPGPIIYKQVRITKNQKEFSIYKFRSMTATAEAKSGPVLAKSNDARVTPVG
                     KFIRAVRFDELPQIFNVLKGDMSIVGPRPERPFFVDQFNAENPYYYLRHNVRAGITGY
                     AQVYGKYVSDYNSKLRFDLLYIKKYSLMLDLKILLQTIKILFDKMSSQGLEEDENVIS
                     LAEIAFPNEKIFR"
     gene            31532..32572
                     /locus_tag="U2K71_001066"
     CDS             31532..32572
                     /locus_tag="U2K71_001066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM7315169.1"
                     /translation="MNNPKFSIIVPVYNAEATIGRTIDILKELDYEEYEVVLVNDGST
                     DQTQEIIQELIENDERFELITTTNQGPGLARNEGIRQSKGEYLLFFDADDTPVKTILS
                     DYDHLLEKSPKADLVVSSFVFRTMDEGRIVSEKENLVTECHYLTHEEFIKDMYQLMNE
                     QLMYVVWNKCYRSDILKNNQILFKTYNSCEDRIFNLHYYEYCHQVIMNPKVEYIYEFE
                     GGKGITNQYRPNKFETFKEFYSLANTVTDDINKDGMAALLLKGTTSVIFSIYSTSART
                     SKEKKDEAKQILSDPVIREAKQIAKTDSSAKKITKLLYNMPAPLFLSAVKVGSFVEVK
                     MPGLMAALKRVY"
     gene            32591..33676
                     /locus_tag="U2K71_001067"
     CDS             32591..33676
                     /locus_tag="U2K71_001067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEM7315170.1"
                     /translation="MKRILHFQGRMGLGGAESFMMNLYRKIDRTNYQFDFLIYEDYAD
                     VQDYHSEIERLGGRIFVVPNPKKNIFKYLIEVNKLLKKESFSIVHNQVYFGGGINLWL
                     AKKNGIRQRIAHSHATEDGKSQNIVMNVLRKFLTKLLLQNATDYLAVSQEAGESLFQN
                     HPFEIVHNGIDLELYSKNSEAKVNKRKELDISMSTFVVGNIGRLEKQKNQSYLLEIFL
                     EILKMNSDSYLILVGEGSLRNKLKAKAVELDIEKRVKFLGERNDIPELLSVMDVFVMP
                     SLYEGLPMVGLEAQAAKLKLVLADTISKDTKLTNNVDFVSLDETPQEWAKKVIKEPYA
                     NKITQELKSYDVSYTTEQMESIYSRDH"
     gene            33709..34671
                     /locus_tag="U2K71_001068"
     CDS             33709..34671
                     /locus_tag="U2K71_001068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEM7315171.1"
                     /translation="MKICYLILAYNDIGNLKRLVNRLNENAVFIIHIDKKTDIEPFIE
                     AFKSYDNVEFLCNKNRVKIYWGGFSIVQAEINLVKRALQNEKYLKYVLLSGADYPIKD
                     NEYIYNYFKKNSSVEFIRGIDLDQIKHKEFYYKHIDVYQKHDYPRINRNNTTAFKIFR
                     AIINRCLRMIKLPPKIRHHKFDLYHGSQWWALSKECLTELIQMYEQNQQDYLNFKIGM
                     FAPDEKFFHTLFFNSSFKNKNVIGGPDLPLELKNIEETTLQTSKLANIHIIDPSMNKW
                     FDERDIDQIKESEKLFVRKVKSDYSDKLLNRIDKEILKVGNIDE"
     gene            34664..36091
                     /locus_tag="U2K71_001069"
     CDS             34664..36091
                     /locus_tag="U2K71_001069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HEM7315172.1"
                     /translation="MNRIVPVLEKSLLSRDAFRVINLIICLLYFMPSTFNVTNIPMKI
                     AFAWGGIIVLYEVFIKKNFFKMRFSWLLIGAMISFILTIIINRENFLIPSIYNFGYLL
                     ITLIVIFPADFSQSEEEKKKKMVYFNNVFIIIIFLAALISIYQFIFLISYHVPTGTPG
                     LLARQGFIEHRLFGVYTSPNVGAMFGYTSIILSLISVLISKITKTIRVFYMMNFIVQI
                     FYITLSSSRGAQVVICSFLVLFLLLFANSKFRKSIREVLPVKIKWMVSILLVLLVYFS
                     FGTNYAKDFLATIPVSIDRKVEVIESNESDKTPLPEKEVILQHSSDNSEISAGRFTIW
                     KAALKVIRPTLLFGYGETDFYGNSDAKFLKKVDLDTTDVNELKRAHGNMHNGFLQVLV
                     SSGIIAFICIYAFYVLNIFSLLKTFFRSKVNYAFLGIVLIFILSIFIDEMVEAHVLFN
                     KRDVISIVFWYYLGSISYLYLKKEN"
     gene            36093..37163
                     /locus_tag="U2K71_001070"
     CDS             36093..37163
                     /locus_tag="U2K71_001070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315173.1"
                     /translation="MKTAFIAWTPLHLINILNVYQNFYRESKGDLFIYSEFENARRYS
                     ERIKELGLFNDVVFVNHRKIGSSFKRKINLLTNHNQFFSDDLSVYDEIFIQGENYFTK
                     ILFSNVYKKNPDVKLNYIEDGLGTYLKKSIFSTSKNVQRVFKILNKNSIFKKKIDDYF
                     VYCPELLLYDVKNIKKIPKIDENSKLMKNIQSIFGIEEVFVGDKCIFFDQPLLSDGYG
                     IDETDIFAKVNSIFSGKKETLIKLHPRARTNRYGEKNILNSSLPFEVLLLENDFSSTL
                     LISPISTISFSPSMMFEKTINSIMLAKLILNEYTALSEDKKETLTNIVLFCEHYNRLS
                     EEKILLPENWAELRKLVEYGII"
     gene            37150..38571
                     /locus_tag="U2K71_001071"
     CDS             37150..38571
                     /locus_tag="U2K71_001071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEM7315174.1"
                     /translation="MGLFNTLSKSKLVKSSFWYTIGNFFIKGISFITIPIFVNITTQA
                     EYGLINNFTSFVSIFSILVGLSLNSAINNASFDYKDDINEFMSSVLSLATLSLIVFLL
                     IGNIYFLFSNAFFDMNQLVFNLMLLQSFASFVISYIQSYFTINFNHFKYLILSFISTF
                     LNIAISVALMQTLYFDDKYVGRVVGGTLSFVLIGLVIYLRIMIKGAHFFDKKQWRYAL
                     KISLPLIPHSLANIVLAQFDRIMINSYLGPAAGGIYSYISNIGIILSVIWMSTNNAWV
                     PWFYGEMSKNNEKKVKKVSNYYLVVFTLITMIVMVVSIDLAKFMAPKDYYSGLSLVVP
                     ITLGYFFQFLYSLPVNAEFYEKKTSFIAIGTVASAIVNILLNMIFIPRLGIIAAGYTT
                     VFTYFLLFIFHYNIAKKITNKQLFDTKKICIIIIFVSIFSLILHILINQMLIRYVLTS
                     LLIISLVIYFLKIKNRIIDDYNS"
     gene            38584..39591
                     /locus_tag="U2K71_001072"
     CDS             38584..39591
                     /locus_tag="U2K71_001072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM7315175.1"
                     /translation="MFKGKTILVTGGTGSIGSEIVRQLLKYEPKAIRIYSRGEEKQFY
                     MQQELSNHTNLRFLIGDIRDSQRIDYACRDVDIIFHAAAMKHVPASEYNPMEAVKTNV
                     MGTQNVIDAAIKNNVKHFVGISTDKVVSPTNTMGATKLLSEKLIIAANQYKGKTETIF
                     SCVRFGNVMGSSGSVIPLFSKQIKNNKDITLTDVDMTRFMMSIPDAARLVLSAAMNSK
                     FGETYVLKMPAINILTLAQSLIKIYNDTHDEKYRGEIKVVGIRPGEKLYEELMTTDEI
                     ERSYENDKMYIIPPHYHATDELYKDYEKIKITAYSSDATELMSGQEFIRIAKENNVVT
                     W"
     gene            39603..40607
                     /gene="neuB"
                     /locus_tag="U2K71_001073"
     CDS             39603..40607
                     /gene="neuB"
                     /locus_tag="U2K71_001073"
                     /EC_number="2.5.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase"
                     /protein_id="HEM7315176.1"
                     /translation="MKSEVYVIAEIGVNHNGSLDLALESIDKAKECGANAVKFQTFKT
                     NKLVSKKAPMAKYQKDNLKEDSSQFEMLKKLELSQSDFIRIKDYCIQNKIDFLSTPFD
                     EESADFLKQIGVQGFKIGSGDLTNLPFLRKIDMYGLPVLLSTGMADIHEVSEAVDCFI
                     DSPVTILHCTSNYPASSEDINLLAINTLKEMFGIPVGYSDHSLGYDVGICAVAMGAKV
                     IEKHFTLDKELPGPDHKASLDPQEFADFVKHIRNTEIFLGDGVKRAMPSELSTKEVSR
                     KSLVVTENLNVGDILTEKSLTIKRPGNGIAPKFLYQYIGKKVKRPISADSVLLEEDVE
                     "
     gene            40604..41752
                     /gene="neuC"
                     /locus_tag="U2K71_001074"
     CDS             40604..41752
                     /gene="neuC"
                     /locus_tag="U2K71_001074"
                     /EC_number="3.2.1.183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (hydrolyzing)"
                     /protein_id="HEM7315177.1"
                     /translation="MKKILIVSGTRADYGIYKPVAENLLQDNWDVSFVVSGMHLSHKY
                     GFTKELILADGFRISGEVQSLFLENNKGNMARSVALEINGFTNIFEQTSPDFVLLLGD
                     RGEMLAAAIACLYLGIKTAHIHGGEVSGTVDESIRHAITKIASVHFAATEKSKERLIK
                     MGEDSWRVFPVGAPRIDTILNNRLPSFRDIKEKYHLKFEKKKYVLQVFHPVVTELENI
                     EKQILALINSMEQQSQPIICILPNSDAGSEIIRKMYQKYLKTDIIYITNLEPEEYLTI
                     LKECKFMIGNSSSGIIEAASFKIPVINLGTRQNGREQSKNIINANLNENEIETALNKI
                     QDPQFIKSLKNVKNIYGDGNSSRRITKTLNQILNDGEDIKWIQKQIAY"
     gene            41725..42402
                     /locus_tag="U2K71_001075"
     CDS             41725..42402
                     /locus_tag="U2K71_001075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HEM7315178.1"
                     /translation="MDSKTNSLLKYTSDPYIIIGAGGHAKVIIETLRLENKLIYGFFD
                     DKQNKMQDLKYLGKINELNSWADSKKKIYKFIVAIGDCVVRKKIIKKLNMPSEQYGIA
                     VHPSAIVAKEISIGYGTVIFANAVINPDAVIGEHAIINTGSIVEHDCRINNYVHLSPG
                     VCLAGGVHVGVGTQIGIGSQCIQMKKIGSWCMIGAGSTIVKDIPSHSLAYGNPAKIKK
                     EGINFEF"
     gene            42392..43534
                     /locus_tag="U2K71_001076"
     CDS             42392..43534
                     /locus_tag="U2K71_001076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HEM7315179.1"
                     /translation="MNSRILLSSPHMGGTEEKYVAKAFKDNWIAPLGENVDKFEESIT
                     KYTGSKGGASALSSGTAAIHLALRLLNVGKNDIVFAPSFTFIATVNPILYQGAVPVFI
                     DSEPNTWNMSPVALESALEEAERNNQLPKAIIVVNLYGQMADYERILAIANQFNVPVI
                     EDAAESLGSTYKGISSGLFGTIGIYSFNGNKIITTSGGGALVSSDKKYVEHSRFLATQ
                     ARDQAPYYQHSEIGYNYRLSNISAGIGRGQMEVLNDRVAKRREIFNRYKEAFSGIDRI
                     EMMPEVDGMVPNRWLSTLTINDYNSIDQVVNVINKLNEKNIETRALWKPLHLQPVFQG
                     VKMYYHDNNLSVSEKLFNSGLCLPSGSNMTDSEQEFVIDEFKKIFK"
     gene            43547..44524
                     /locus_tag="U2K71_001077"
     CDS             43547..44524
                     /locus_tag="U2K71_001077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM7315180.1"
                     /translation="MNIGIIGLGSMGKRRLRLIQEHFNDILLCGVDTNEARRKEVEKE
                     FGIPTYNSLTEAKNLFEINSVFVCTSPITHEFIIKEALNLDCNIFTEINLLNEYYDEI
                     IEVAKKKDRLLYLSSTFLKRKEIQYIKSKVTHNKKISYQYHVGQYLPDWHPWEDYRNF
                     FVSNKKTNGCREIFAIELPWIIDTFGKVKSFTVMTQKLSNLDIDYSDSFTLIIEHETG
                     IVGTLSVDVVSRVAKRNLTIIGEKILIEWEGNPDSLFSWNEETSSMTDIQLYDEVMHD
                     NNYSHTIIEDAYLEEIKEFFTLLEKKMEPQYTFEKDKYIINLINDIEVN"
     gene            44524..45432
                     /locus_tag="U2K71_001078"
     CDS             44524..45432
                     /locus_tag="U2K71_001078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydrogenase"
                     /protein_id="HEM7315181.1"
                     /translation="MMKVIFFGLGSIGKRHLKNLISYGKKNSINFDITAYSSSGKKEF
                     EEITYIQNRDDLDTHYDIAFITNPTFKHLETLNVIVEKVDYIFLEKPAFSESIDLTQY
                     NYDYKNIYVAAPLRYKNIMEVVRNSIQSKKVYSVRAICSTYLPDWRKGTDYRNNYSAF
                     KSMGGGVELDCIHELDYIINLFGFPKSVKKQFGKYSNLEINSNDLATYILRYDDKIIE
                     IHLDYFGRVPTRKFQLFTLDECINVDLLNNKISFEENIVKEMPESANDMYEKELKYFF
                     ENVVTGKENWNDLYHANEVLKIAEEK"
     gene            45433..46185
                     /locus_tag="U2K71_001079"
     CDS             45433..46185
                     /locus_tag="U2K71_001079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEM7315182.1"
                     /translation="MEILFTICGRAGSKGFKNKNLKYLAGKPLVHFALAMIDIYQQKH
                     PEHNCTTCLNTDSNELVELTTKLNPSVKIVERKPELATDTVGKIEVIRDSFLQLERED
                     NIFDLIIDLDLTSPLREVSNLEELIDEHLKNLDKDVYFSVVSARRNPYFNMVKRIENE
                     VTIVNKSRYTSRQQTPEVFEMNASMYSFKPEFLKKSNYIFDGQCGIIKMRDYLILDID
                     SEEDFKWLEFLFPKFLEDSSELQDIYNYLYRR"
     gene            46190..47137
                     /locus_tag="U2K71_001080"
     CDS             46190..47137
                     /locus_tag="U2K71_001080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HEM7315183.1"
                     /translation="MSCYLITGGAGFIGSTLANHLGEEHVVIVVDDLSMGKVENLDMT
                     KNITFIEGDVAEKSLMEEIMKANKFDYIFHLAAVASVADSVARPVETHRVNFESVLMI
                     LELIRKYQPDLKRIVFSSSAAVYGDEPTLPKKEESVIRPLTPYAVDKFAAEQYVLDYC
                     HLYDVPGSAVRFFNVYGPNQNPNSPYSGVISILVDRYKKQIVGEATSFTLFGDGSQSR
                     DFVYIDDVIQALLLVANEEAALGKQFNVGTGKATTLLELIHAINEILGVELTLEYKEE
                     RSGDIHDSLADITKIQSIGYQPKYGILSGMTNYLKTEIN"
BASE COUNT        15836 a         7485 c         9104 g        14949 t
ORIGIN      
        1 tattttttgc atgtgtgcga aagtatactc aagcccaatc gccatttcgg aaaatcggta
       61 ctgtagagcc atcttcacgg attccgtcga tatccatctt gtcagatcct accatgaaat
      121 ctacgtgggt ttgacttcta ttcaatccag cttctgctaa ttcttcatcg gacatttctg
      181 taccgccttt cacactgaag gcataagcag aacctagtgc taagtgattc gatgcgtttt
      241 catcaaataa tgtattgaaa aagatgatgc cagattgaga aataggtgaa gggtcaggta
      301 caagtgcgac ttctcctaaa cgacgcgcac cttcatctgt atcaagcagt tttgctaaga
      361 catcttctcc ttgttcggca gaaaaatcaa caacttttcc atctttaaaa gtaaatttca
      421 tacccgagat gatggtgcca gcataactca gtggctttgt acttgaaatg taaccatcta
      481 ctcgatgact gtcaggtgct gtgaacactt cttccgtcgg catatttgcc atgaattttt
      541 ctccacgagc attatagctt ccagcgcctt cccaaagatg gtttttaggc aatccgataa
      601 tgatatctgt accgggagca gtatagtgaa gggcagaaaa ttgttctcga ttcaattctt
      661 ctgctttttt tgctaatttt tcatcatgct tcttccatgc taagacaggg tcttcttcat
      721 agacacgagt cgttttgaaa atctgatccc ataatgcctc aacttgtttt tcttctggca
      781 gttctgggaa gacttttgct gcccattgtt taccagccgc agccacgaca gtccaactga
      841 ctttatttgc ttgagttgcc ttgcgtaaat tcattagtgc tttgccattc gctgattgat
      901 aagacgctac tcgatggcta tctacaccag caaatgcatc gggatcggca gaaacaacgc
      961 tgatcctgct tgctcctttt tcaagccatt catccgcttg atcgatttta gattgtggta
     1021 cattctcaat acgatccgtt gcagcatgta aaagaaattc tctttggatc tggtcgtcag
     1081 tccattgaac gataacctca gcggctccta gtttataagc ttcttgtgtg atcaatcgag
     1141 caaggggtgc ctgttccacg ctgatctgta aaacaactgt gtgtcctttt tctgtggcta
     1201 cgccagtttc tgcaattaag cgtgcatatt tttttagaag ttcattaaaa tcagataaca
     1261 taataaaaat tcctcctcac cattttttct ggtaactatt agcatgatac catttaatta
     1321 tgattcaaac aatgaggctt taaataaaaa aaggaggtta tttttttaag gttaagtttc
     1381 atgatatatg ttactacttg ctatttcagc ttgtgaagaa aatcaaaaac taattttgca
     1441 tatcaaaaaa aacatgttac aatgaaatta atcaatggtg gaagcgttat aaaaaaatta
     1501 aggagattaa aaaatatggc aagaaaaaag acgattacta gagaacagat tttggcagct
     1561 gcttatgaag tagctgccac cgatggattt acacgattta ctgcgagaaa catcgcgaac
     1621 aaaatgaatt gttccacgca accgatttat cttgagttta aaaatatgga tgacttgaga
     1681 caagctcttt ttgagcagat ctatgatcat ttacaatatg aagtgttcac tgttgagcat
     1741 acagggaata cgattatcga tcttgctttt aattatatcc attttgcaag aagagagaag
     1801 aagttgtatc gttcactgta cttagaagaa tatggtggag gaaaagagat gcagaatttc
     1861 tcttatactt actttgtgaa tacagtgaaa cgcgatccag cttatgtgaa tctttcagaa
     1921 gagcagatcg aatctttgta taatgggaca tggatcatag ctactgggat cgcagctttg
     1981 atgtcttcaa gcatcatcca tccaactgat gaacagattg aaaagatgat ccaagacagt
     2041 atagatgcta tcttgaaatt caaagaacca atcgaaatcg attgaatata ctcaaaagtt
     2101 ccacaaatca gtaggtaaca agagatgtgt caaaataagg aaaagttcgt ttttcttaca
     2161 gcgtatgcat ttttaactcc cgaagaactc ttccatgaat gcaatgaata aaagagccat
     2221 acagattgaa taaatctgga tggctctttt attgcaagta tattaaattt ttgcgactag
     2281 ttctttagcg aaagcttcta aattttcgat atcttcttct tcagcattca gatccacttt
     2341 aacattttcg gctccttttg atgcgcctgt ttttgcaaaa gcagcatcga aatcatccac
     2401 cgatttgcag aattcatcgt aaaaagtatc accagatccg cagacaccgt aaatttttcc
     2461 caatagatcc aattcttgca agtcttcata aaagtctacg atctcatcag gcagatcgcc
     2521 atcatcgtat gtataagtag ctactacgca aatatctgcc tcctcgaatt catctgcatc
     2581 tacttgggta cattcgttga tttcgacttc gatatccaga ttttcaaatg cttctgcaac
     2641 aatatcggcg atttcttcag tgtttcctgt catgctagca tatacgattt ttgctaaagc
     2701 catgatgtgc ctcctcgttt ataattattt aacaattatg aattatacca aaacatcatt
     2761 gaaaagaaaa taaaacctag agacacctgg ggaatgtcat ttggctaaat gtatgccgct
     2821 atgttaaaat gagagggatt tgtaaaaagg agacgaaaat atgattactt taaaatcagc
     2881 acgtgaaatt gaaatgatgg cagaatctgg agctttatta gcagatgtac ataaaaatct
     2941 gcgtgatttt ataaaaccag gaatcacaag ttgggatatt gaggtatttg tcagaaacta
     3001 tatcgagagt catggcggaa ttgctgcgca aattggcttt gaagggtatg aatatgcgac
     3061 ttgtatcagt ataaatgatg aaatttgtca tggattccct cgtaaaaagc ctttgaaaaa
     3121 cggtgatttg gtaaaagtcg atatgtgtat cgatttgaaa ggcggcattt ctgattcttg
     3181 ttgggcctat gtagttggag aatctacacc tgagatcgac cgattgatgg aagtaacgaa
     3241 gaaagctttg tacatcggta ttgagcaagc acaagtaggg aatcggattg gtgacatcgg
     3301 tcatgctatc caaacttatg tagaaggcga aaatctagcg atcgttcgtg attttattgg
     3361 tcatggcgtt ggtcctacga ttcatgaaga accagcagtt cctcattacg gagaagctgg
     3421 aaaagggttg cggttgaaag aaggcatggt tatcacgatc gaacctatgg ttaataccgg
     3481 aacttggaaa atgaaaatgg atcctaacgg ctggactgcg tatacaagag acggcggact
     3541 ttcttgccaa tatgaacata cattggctat tactaaagat ggtccaagaa ttttgacatc
     3601 acaaggagaa gaaggcactt attgatctat ccagtcaata ggtacgggag aggtacatga
     3661 aagtttggaa caaaataaaa caaaatgaaa atctgatgcg ctttattgag acgactcaaa
     3721 agcgcatcac tgattcagaa atgagtacga cctctgtcgt cgttgcttat tatttactct
     3781 tgtctttatt tccattgatt attgcttttg gtaatatttt accgtttctc cacatcgatc
     3841 aagagactgt actgacctat ttacgagaag tgattcctga gaccgtttac cagtttatcg
     3901 gtcctgcaat caaagacctt ttgacacaaa gttcaggagg cttgttatcc gtttctgcat
     3961 tagcggcttt atggtctgca agtcaaagta tcaatgcttt gcaaattgcg atgaataaag
     4021 cgtatggcgt agaaaataga aaaaattttt tgatcgtccg tttcttctca ctaattgtca
     4081 ttgtcttgtt catgattgct attagtgggg taactatcgt tcttgggttg ggacagctga
     4141 ttttggatgc tattcagcca atcgtacaaa tacccgtgaa ctttatcgat cagttccaaa
     4201 ctttgaagtg gccaatcaca ttggtagctt tatttgtcat catgttcttg atttatttaa
     4261 ttgtccctaa tgctcagttg aagttgaaga cgatcattcc gggcacgatc ttcgctactg
     4321 tcggctggat gctgttatca caagtgttta gtatctatgc taggtatttt gcaacgagga
     4381 tcagcggtta tcagatcata ggaagtttta ttgtactcat gctttggctt aattttgctg
     4441 ctacgatcat cattttgggc ggaatcatca atgcagttgt tcaagaatac gtgacgggtc
     4501 atcaaatcaa aaaacgtcga gatccaagcc gaaaatggtt caataaattg aaaactaaat
     4561 tttctcataa aaaaaagtag aaacaagagt tcttattgta aatttatatc agtaatttat
     4621 ttgaaatttt atatctaatt ttcaattatc cgcataaaca ctatacattc aagcattttg
     4681 aagtatagaa aaacactcat cttaccacga atttaccacg attgaatgag ccttgatatg
     4741 accgtaaaac tactaaggga gatagcacga aactgtgtat ctcccgattt ttttatacag
     4801 ttgattactt cactccgatt tcaaatttca atattgcttt catcatgtct gtatgaattt
     4861 ctccttttag ttcttcatca acctgcataa tgattttacc actttcagag tggtaaaaag
     4921 gacgcaagca taatttagaa atataaccgt cataaaactt caaaatctct ttaactgcca
     4981 tttcatctcc gcttgacgct ttgcaaatca attcaaaaga gggatagcca taatctttac
     5041 tcatttcttt acttcctttc gctcaaaagc atttttaact tccttagtcc tctagttctt
     5101 ctactgcaaa tagcagaacg tgatacatgg tacagttctg aaatttctct gtcgttcatt
     5161 ccttgaaaat aacgtaataa aatcgcacta cgctgttttt ctgacagtct atgtaaaact
     5221 gtaccaagtg tttcatcaga tacataaatt gtaaagtgca agactttaaa tgatacacaa
     5281 tcataaaaac aggtatcaat cgttccacgt tcaacttgtt tcgtttcggc tatttcacaa
     5341 aacaatactt cacgtttaga atgtcttgca agctgtctgt ttcgactttt cactttgttc
     5401 ttaatgacaa tcagcattaa agaattgaac tgtaatctga cgatatgctc gaaagaagat
     5461 gtcctcataa tctcaccccc tttctgctaa tgctgaaata tataaacgga ataccgttta
     5521 taccctttag aaatacagaa aaatggtaag aaaaaagccc acacaaaaca tatatttgtg
     5581 cgagccttaa atactgcaat attactcttt cttttataac actatccttt tagcagattt
     5641 caaattttct tttatatatt gtttttagcc taacatacta tttaacccct tgtattatat
     5701 ctctaacttt tgaaaaattc tttttgaata tattgaaatc atgattgaag caatagaagc
     5761 tacaaacata acaacccatg tttcttgtat aaatacatcg aataaaatac cgtaaaccat
     5821 ttgtccaata ggttgaccgc acatagcaac tgctaccagc gtagccatta tctttcctac
     5881 taattgcggt ggtgtttgtg tctgaacaag agtgtaaatt tgaacaacaa acatagtaga
     5941 tacacccata gccacaaagc acatcactgt aactacccag taggaaacta ttgcaggcaa
     6001 ttctaaaaat agcgaaattc ccataagtaa aacagaaaca gaacaaataa gtaacaaggt
     6061 ataaaggttt tttagtttta actttgaagc aacaagagct gttaaaatac caccacataa
     6121 accacctaat gataatgctg attgtgaaaa accatatagt gtatctggca tttttaaaac
     6181 ttcagaaatt aagataggca gaccaacaat taaaacagca cttaaaacca aattgaaaat
     6241 gcaaaccaaa aagattacgg atataaaaat gggtttctcc tttttcaaaa aggtataact
     6301 atcttttaaa tcgtttttag caactgcaat tacacctact tcacgaggtc ttttttggtg
     6361 tggaatatgg ataaaaattt ccataatggc tgaaaaagta aaacatataa tactaacaat
     6421 taaaatggga ttgataccat aaaattgaaa aactactccg cttatagctg gtccgataaa
     6481 gtttgctaaa gtatttactt gatttattat agcattaccg acggtaagtt tatctgatga
     6541 taccaataga ggaatacttg cttgtaccgc tggttgatat gcaccagaaa taccataaag
     6601 taacatcaaa gcaacaataa acaaaggtat gataggaatt tttcccaatg acaaataaaa
     6661 tactaaaatt atgattgctg ttacaaaatc tagtatgacc ataatgtttc ttttatttgt
     6721 cctgtcagca ataataccac ctaccagcga caaaataacc ataggtaaaa aagagcaggc
     6781 tgtagcaata ccaaacaaag ctgatgaacc agtttctttt aacaaatagt acggtaatgc
     6841 aaatcgtagg atagcgtttc caaataaaga aattatctgt cctataacaa caagtataaa
     6901 atcacgatta aaaagttttt tttctgcttg cataaaatct ctccttttta tttaatcaaa
     6961 cagaccgtcg gtttatataa tggtttaaat aaactgctct tttatagagc agtctatcgt
     7021 atagggataa caaatacagt tatttacaaa actgtataaa tgcagaaatt gtttcttcgt
     7081 ctaataatgt gtctatgcct attccattta aaagggcatt gaatgtattg caacgttttt
     7141 ccataccctc tttatccgtc atttcaacta atgggttgag ccatacatta cttaatacca
     7201 tgataacttc tgcaatttca tgtgaatttt caatctccaa agacttatcc ttgatacctt
     7261 gttctaaaac gggttcaaca aattcaggta ctacaatttc atacatttgt tctatttgca
     7321 tagcaagaaa acgaggattg tttaatagtt gaggagaaac tgtcaataaa acgctttggt
     7381 tagaattgaa taccgctgtc ttaaatattt ctcttaactt ttctaaaccg tttaaactat
     7441 cgttatctct aattttagca aatattactg cattttcatt tccaattctt tgaaaaattg
     7501 cttttaaaat atcttccttt gaatcgaaat gatgataaat agcaccttta gaaagttttg
     7561 tttcattgat aatatcttgc aatgatgtgt tatcatatcc tttttcacaa aacatttttg
     7621 tagaaacatc taaaatgagt tttactgttt cttctggata tttatttcgt gccataactt
     7681 acctccaata caaaccgttg gtctgattaa agtatatctt agtattttat gcttgtcaat
     7741 ataaagactt gcaattttat aaaaatgcag aatgcaagcc tttttcattt attctacaat
     7801 cttccagttg ctttccttat gcggtgtcaa ttcatactgt gatacctgcg ttgcctttgc
     7861 ctgatttacc acaaatcaga ttttatccct ttcttcctct tgattgccca caacagaaaa
     7921 gaaaaagccc tgtcaagagc tttgccacca ttggtggtgc tttgcactct tgatcgggct
     7981 ttttgtttgc tgtatcttct cgcaagagga aaaaagttac tcgtcctcat catcaaaatt
     8041 gttattcctt aatacttctc gtaacagttc catatcttct tttgagttcg ggtttatcat
     8101 tttcacgact tgataaggtg tcttatactt atgtccctct atgctttccc caaacatatc
     8161 catactgtat aaatcatcat agcggtctaa taatttttga aacttcaaat gctggataaa
     8221 ttctttgatt gggtaaaggt cggacgcttc aatgcttttc ccgttgatat agtctgtaat
     8281 atattcccgt aacttttcta actcatattc cagccattct tcctcgctgt caaagaagtt
     8341 atcatagtgt ccatgtttca gttctgcatt tagacgttct tcggctctta aaaactgaca
     8401 gacttccttt tcagattgat tgacatactt ccattctccc gacaacaatt cattgggcgt
     8461 aacgtccagc acttccatta tcttttccag cgtttcatag gcaggatagt tcaaccctct
     8521 ttcaatcttg gaaaggctct gcatattgat accgattttg tccgcaagtt cctgttgttt
     8581 cattccccta aattttctta tggtctgtat attatttcct aaatggctta ttttcattgc
     8641 ataaccctcc atatctcgtt atatcttcgc tattataagt ctatcatgct taatatgatt
     8701 tgtaaaggca aaaaaagata ttgacaaata agcatataag aattactatg ttcttgtaat
     8761 cgaataagca caatagaatg aaatataaaa acattcaaaa agaaatacct atctgattaa
     8821 atccgtagat atgaatatcg gttgaaatgg aacttgttag aaaaggcaaa acagaacttg
     8881 ctgacaaagt atttctgaca cggtggcgga agcctctcag gcttaccgcc atgacacctt
     8941 gtgtcaaggg gaagaaatgc tttgtcggct ggcgagcctg cgagccttgt ctgtgccccc
     9001 gttatctaac aggggggcac tatttcacaa aagacaagtc aaaaaccata cacccccaaa
     9061 cctgtgagta gtaatgagag gttttaagaa gatagtacat atcacacttt ttgaaaggtg
     9121 gtttacaggt atttcaaggc tattgtggga ttggaggaat acaatgaaca atttagaatt
     9181 tgcacttgaa atgaagaaca aacgtcttgc cagcggtctt tcacaaggag aacttgcaag
     9241 tcttacccat gtatcaagat atagtatcaa ccgctttgag aacggaaaag ctaacgccag
     9301 caaggaaaca cagaacatta tcctgcgttg tctgaattac tatatctgcg ataagccttt
     9361 ttatctgctc gttgattatc tgtctgtccg ttttccaacg actgacgcat tggaagttat
     9421 ccgaaaggtg cttggcatga aagcagatta ttttatccat tatgactatg ggtattatgg
     9481 ctataaggag cattatgctt acggggaaat caaggtcatg gcttctgatg atgaacacat
     9541 gggcgtattt ttagaattaa aaggggcagg ctcacgcaac atggaatatg tcttacaggc
     9601 acagaacagg gactggtatt cattcttaaa ccgctgtctt gactgtggcg gtgttatccg
     9661 acgttttgac ttggcaatca atgatatgtg cggtctgctg gatattcctg tgctatctga
     9721 aaaatacaga aatggtggtg cggaatgtcg ctgtaaaaac tatgaaaatg tacaaggtgg
     9781 aaaattaagc ggaaaaaacc gtaatttagc aagtaccctt tatatcggct caaaggcaag
     9841 cacaaaatat ttctgcctgt atgaaaaaca aaaggaacag gcaacgaaaa agaaacatac
     9901 cgatattatc aaccgttttg aaattcgtct gcgtgacaaa aaggcggtac aggcagttga
     9961 agaactgtta ttgacgtata accctcatgg gctggtgttt tatctcatta ccgattttgt
    10021 ggaatttcct gactatccat tgtgggagat attcatttcc catgacagtt taccttttga
    10081 aatgaaccct gtacctgtca atatggaacg cactctgcaa tggctggaaa gacaggtcat
    10141 gccgtcaatc gtgatgatag aggaaatcga caggctgaca ggttcaaact acatgaaaat
    10201 gattgatgaa tgtacccacc tttctgaaaa acaggaaatg cttgtggaac agatgtgtac
    10261 ggacatatca gacgtaatcg aaagtgaggg ggtgttttat gagtaataca caggatattc
    10321 ccgtatggga aaaatacacc cttaccattg aagaagcgtc aaagtatttc cgcattggag
    10381 aaaacaagtt aagacgattg gcagaggaaa acaaggacgc tggctggctc attatgaatg
    10441 gcaaccgcat acagattaaa cgccgacagt ttgaaaaggt tattgataaa ttggacgcaa
    10501 tctaatgcaa atgagccttg tatgtgttat gatgaacaca agtcatatca aggctctttc
    10561 caacaaggaa aggagcagac accatgaaag aaaaaagacg ggataacaaa ggtcgtatcc
    10621 tgcatactgg agagagccaa cgaacagacg gaaaatactt atataaatat gtagacgcat
    10681 tgggaaacac aaaatatgtg tatgcttgga gattgacacc cacagacccg acaccaaagg
    10741 gaaaacggga aaaaccctca cttcgagaac tggaacagca gataagacgg gatattgagg
    10801 acggtatcga cagcacaggc aagaaaatga cgctttgcca actctatgcc aaacagaacg
    10861 cacagaggac aaatgtgaag aaaagcacac agaaacaacg ggaacaactc atgcggttat
    10921 taaaagagga caagttaggt gctaggagca ttgatacgat aaaaccctct gacgctaaag
    10981 aatgggcgtt acgcatgaaa gacaaaggct tttcctacaa taccattaac aaccataaac
    11041 gctcgttaaa agcgtcattc tacatagcca tacaagatga ttatgtgagg aaaaaccctt
    11101 ttgatttcaa gctaagtgaa gtcatagaaa atgataccaa agagaaagtc gcattgacag
    11161 aggaacagga gcaagcctta ttgtcattta ttaagacgga caacgtgtat cacaagcatt
    11221 atgatgatgt gctgatactg ttaaagacag gacttcgtat ctcggaactg tgcggactga
    11281 cagtagccga cattgatttc aagaatgaag ttgtgattat cgaccaccag ttactaaaga
    11341 gtaaggaaca gggctattat attgaaacgc ctaagacgaa aagcgggata aggcaagtgc
    11401 cattaagcaa agaaacgata caggcatttc aacgggttat caacagccga ccaaagataa
    11461 agtcgataga ggtagacgga cacagcggtt ttgtgtttat caatccgaaa ggtaatccaa
    11521 aagttgccat tgattataac gccttattta tccgtatggt aaagaaatac aacaagcacc
    11581 acaaggacaa tcccttgcca catattacac cgcatacgct acgccataca ttctgcacaa
    11641 ggctggcaag caagaacatg aacccgaaag atttacagta tatcatgggg cattcaaaca
    11701 tcagtatcac aatgaactgg tacgctcatg cgtccataga taccgcaaaa tcagaggttc
    11761 agcgtctaat cgcatagaag tatttaccac gattttaacc acgattgaca gggaaaatat
    11821 aagaagatag acctagatat gcgaggttta ccacaaaagc aaaatgcccg tagagccgat
    11881 aaaataaggc tttgcggaca tttgagaaga tataaaaaga tagtcaaaaa gacatatata
    11941 atttataata attatgactc ttaattaaag taggtgccca tatgcttgtt tcttttagta
    12001 tggttataag attttttctg acaattttat tatcactgat attaacacct gtattcaaaa
    12061 tcatttcagt acaaacaggt atggtcgaca aaccaaatga aagaagaata aataaagttc
    12121 caatgccttc agcgggcgga ctgcctatct ttatttcatt tgttatatcg actttattct
    12181 tattccgtaa aattatccct caatcgtata ttgtaccgat cttgattgct tctttagtaa
    12241 tcatagtcac aggagtgctc gatgataaat atgagctgac acctaaacaa aaaagtatag
    12301 ggatcttgat tgcttctctt attatttatt ttgtagcaga tatacgtatt gattccttca
    12361 cgcttccttt tattggatat atccaactag gctggcttag ttttcccgta acgatttttt
    12421 ggatcttcgg tatcacgaat gcggtcaatc tgattgacgg tttggatggg ctggcggcag
    12481 ggatttcatt gatcggttta attacgatag gaatcattgg atattttttc ctccatgctt
    12541 ctacagtgta tattccagtt gttattttct gtcttgtagc aagtattatc ggtttttttc
    12601 cttacaattt ttatccggca aagatttatt taggcgatac aggagctcta ttcctagggt
    12661 ttatgatggc cgtcctttca ctacaagggc tgaagaacgt gacttttgtt tcatcgattt
    12721 ctttgctgat cgtcatggga gtgccggtaa cagacacgtt ttttgctatc attaggagaa
    12781 aagcaaaccg tgtttctttt tctacagcag ataaaaaaca tcttcatcac cgactaatca
    12841 gtttaggctt tactcataaa ggtgctgtcc tgacgattta tgcgatggct ttgatgtttt
    12901 ctttcacagc tatggtcatg aattatactg gaagaatcgg aacgatcata ttgattattg
    12961 ccatgttgtt tgctgcaatt ttattatttg aattgattgg tctgatcaat gagaaacatc
    13021 agtttatttt aaaaacattg cgatttttag ggaataaaga gtaccgtacg caagtaaaac
    13081 agaaatattt gaagaaatgg aaccgtcata tagatgaata acaaattaaa ccagccgatc
    13141 cttattagta ttgtgacggc aaacagtgat aaaatttttc ggacactaga taattttatt
    13201 tcaactgtaa aagatgaaac agcagtaaaa atccgaattt ttgacaacaa ttcacttccc
    13261 gagtacaaaa aaagactaac agagtatagg aaatatccct acatcgatat ctattttcat
    13321 gatgaaaatt ccggatttgg ttatggacat aatcacaatc tgatagctag cgattctcgc
    13381 tatggcatta tttgtaaccc agatatttta gtggatgaag agacagttac agcattggtc
    13441 ttgttattga aagaacatcc ggaatgtgcg atgttagcac ctaagattct taatgaagat
    13501 ggaacaactc agcacctcat ccgtgagcag ctggctgttt ttgattacgt tttgcgcttt
    13561 gtgcctttca aatgggtcaa aaaaaagttt gacaaacgac tggctgtgtt tgaatgccga
    13621 ggattgccta ctgatgctaa tagttatgta cggatgatct caggtagttt tatggtagct
    13681 gatattgata aatttaaaga gataaagggg tttgatgagc gtttcttcat gtactttgaa
    13741 gacaacgact tgtgtttgag ttttgaaaaa gcaggttaca agttattgta cacacccttt
    13801 tatgcagtta tccattttta cgggaagggt gcgcatcgaa atgcaaaact gtttcgcgtg
    13861 tttttgactt ctatgatgaa atttttcaat aagtgggggt ggcgtttttt ctgatgaaac
    13921 cagaaatatt agtagtcgtt gtgctttatc agcaactatt cagtcaatct ccagcttatg
    13981 gaccattaag caaagcactt agcgaaaagc aaattcagct tattatttac gataatagtc
    14041 ctcaaaggca gcaaaatgag ctgtttgaaa aagaaaatat cgtttatcat catgatccgc
    14101 aaaatcctgg tttagcggtg gcttataatt ttgcgttgtc tcaagcagat gaaggaacac
    14161 gttgtctcgt cactctggat caggatacga ggctagtcga cgattatttt gaactattgc
    14221 gtaaagtaac ctttacagat gaatgtgttg ctgcagttcc gatgattttt tctgggagcc
    14281 gacaagtttc tcccctatat tcagatcgtt atatcaatcg tacagctcaa gcagtagaag
    14341 ttgaaggccc aacagctgaa cgaatcatgg caatcaattc aggtgttgcc tggtctgtca
    14401 aatttctaaa aaagattggc gggtttaatc ctgctttttc tttagatttt ttggatcatt
    14461 ggctgttttg gaaagtggat cagttggaaa aaactgtgga gatccttcct gtacgaatgg
    14521 aacatgatct ttcggtttta gactatgaaa aagtttcaag taatcgatac cgttctattt
    14581 tacaagcaga aaatcgattt tatcatgaat atgatcaaag gcatttatcc agtcaccgac
    14641 gacaattatt gttaagaaca gctaaacagt tcttgaccgt caaaaaccgg catatctggc
    14701 ggatgacttt gcgctcatat gttaagaatt ggaaggtgtg aattcgtgcg ttcggtttgt
    14761 atagctacct ttaatggagg cagatattta agagagcagt tagatagtat tttatctcag
    14821 ctttcagcag aagatgaagt agtcatttct gatgatgggt cagtagatga tacatgggat
    14881 atattaactg aatacgcaga aaaagataag cgaatccagc ttttttcggg gcctcgtcaa
    14941 ggattgatcg ctaattttgg ttatgcgatc cagcagacga aaggagatct aatctttttg
    15001 gcagaccaag atgatgtttg gttggagaca aaagtgaaaa agatcactga ctattttgag
    15061 cagaatccgg atcagctagt agtagtttct gatttaatca ttgtggacag cgagctgcaa
    15121 gagatccatg attcgtattt ttcgtttcga aattccaaag aaggatggct taataatgtg
    15181 ataagaagtc actatattgg tgctggtatg gctttccggt cttctttaaa acccgatatt
    15241 ttgccgatcc cgtcagaagt gccgatgcat gatatgtgga ttggtttgct gggaggaaag
    15301 aagactgggt tcctaagaga accgttaact ctgtaccgaa gacatgattt aaacgcgagt
    15361 gagattgaaa cacattctaa attacggcaa aaaatttctt ggcgtgttca tctgattacg
    15421 gcgttgatca aacgaaaatg gtttaaacgt tgataaaaga tgtttaaatc cttaatattt
    15481 ccatgacaaa tataacaatt gatgagataa taacaaagtg aaatttgaaa tcaaaaagga
    15541 gagcagatat ccatgaaggg aatcattctt gctgggggca gcggaacacg tctgtaccct
    15601 ttaacaaagg caacatcgaa acaattgatg ccgatttatg acaaaccaat gatttattat
    15661 ccaatgtcta ctctgatgtt ggcgggaatc aaagaaattc tgattatttc aacgccacaa
    15721 gatacaccac gtttcaagga attatttgga aacggaaatg atttaggact gcagatcgaa
    15781 tatgcagtac aagaaagtcc agatggattg gcacaagcgt ttatcattgg agaagaattt
    15841 attggagatg atagtgtctg cttggtatta ggggacaata tttactatgg tggcggtctg
    15901 tctaaaatgt tgcaacgagc agcttcaaaa gatacaggcg cgacagtatt tggctatcat
    15961 gtcaatgatc cagaacgctt tggtgtagta gaatttgatg agaatatgca agctttgtcg
    16021 attgaagaaa agcctgctca gccaaaatca aattacgctg taactggcct ttatttttat
    16081 gacaatgagg taatttctat cgctaaaggt atcaaacctt cagaacgtgg agaactagag
    16141 attacggatg tcaataaagc atatttggat aaaggaaaac tatccgtaga acttatggga
    16201 cgcggatttg cttggcttga tacaggaaca catgaatcat tgcttgaagc atctaccttt
    16261 atcgaaacaa ttgaaaaacg tcagaactta aaagtggctt gtttggaaga aattgcttac
    16321 cgtatgggct acatcgataa ggaacaattg ctggcccttg cacagccatt gaaaaaaaat
    16381 caatacggtc aatatttatt gaatttggcc gctgaataag gagtccttac agatgaaagt
    16441 aacagataca aaattaacag atgtcaaaat cgtagaaatg gacgtctttg gcgatcatcg
    16501 cggtttcttc acagaaagtt attcaaaagc aaaatttgca gaacatggat tgaaatatga
    16561 ctttgttcaa gacaaccatt cactatcagc tgaagctggt gtccttcggg ggcttcattt
    16621 ccaaaaagga gaagctgcac agacaaaatt gattcgggta gtcactggag cagttttaga
    16681 tgtagtagtg gatatccgca aaggtagccc gacttatggt caatgggacg gatttatctt
    16741 atctgaacat aatcatcgac agttgcttgt acctaaagga tatgcccatg gattcgttac
    16801 tttgacacca aatgtgaact tcctttataa gtgtgataat tactataatg cagaagcaga
    16861 tgggggcatc gcatttgatg atccagattt agcgattgat tggccaattg atccatcaag
    16921 agcgattatg tcggaaaaag atcaccgtca tccgacatta aaagaatttg aagcagaaaa
    16981 tccatttgtt tacggagaaa tctaagaatt aaggagaaat tttcttatga aaaacatcat
    17041 tgttaccgga ggtgccggat tcatcggctc taatttcgtt cattacgtgg taaacaatca
    17101 tccagaagtg catgtcactg ttttggataa actgacttat gcaggaaaca aagagaactt
    17161 ggctggactt cctagcgatc gcgtagaatt ggtcgtagga gatgttgcag atgcagaatt
    17221 agtggaccgt ctagtaaaag aagcggacgc agttgttcat tacgcggcag aatcccataa
    17281 cgataattca ttaaaagatc ctttcccatt tgtacaaacg aatatcattg gtacgtatac
    17341 acttttagaa gcttgccgca agtatgatgt acgctatcat cacgtatcta ctgacgaggt
    17401 ttacggcgat cttccattac gtgaagacct gccaggacat ggggaaggcg aaggagaaaa
    17461 attcactgcg gaaacaccat ataatccttc aagtccatat tcttctacaa aagctggttc
    17521 agatctgtta gtaaaagcat gggtgcgttc ttttggctta cgtgcaacca tttcaaactg
    17581 ttcaaacaac tatggtcctt atcagcatat tgaaaaattt attcctcgcc aagtaacgaa
    17641 tatcttaagc ggtattcgtc caaaattata tggcgaagga aagaatgttc gtgactggat
    17701 ccatacgaat gatcattctt cagcagtgtg gctgattttg acgaaaggca gaattggcga
    17761 aacctatctg atcggtgcag acggtgaaga agataataag accgtgatgg aattgatctt
    17821 agaaatgatg ggtcaaccaa aagatgcgta cgaccatgta aacgaccgtg caggacacga
    17881 cttgcgttat gcaatcgatt cgacaaaatt acgggaagag ttaggatgga agcctgagtt
    17941 tacgaacttc cgtgatgggt tggctgacac gatcaaatgg tacgaagaac atgaagactg
    18001 gtggaaaaaa gaaaaagagg ccgttgaagc agcatatgcc aaaaatggac aataatacag
    18061 agaaaatgtc actgtattaa tggaaaaaca catgtgaggc aggcgctcgc ctgtctcttt
    18121 tttaaaataa aaatcaagga gcggataatc atatgttttt attaactgga ggaaatgggc
    18181 aactaggtac agaattgcgc catttattag atgaaaaaaa tctgcaatat gtttcaacag
    18241 atgcaaaaga cttggatatc acagatgccg agaaaacaat ggcttacatc acagaattaa
    18301 aacctgatgt tattttccat tgtgcagctt acacagcagt agataaagca gaagaagaag
    18361 caaaagaatt agatgagaag atcaatgttg atggtacaag aaatgtagca cttgcagcaa
    18421 aagctgtgaa tgctcaattc gtctatatca gtacagacta tgttttcgat ggaaaaaaca
    18481 aaaatgtaga atacgaagta gacgaccaaa cgaatccact aaatgaatat ggtcgtacga
    18541 aattattagg cgaaaaagca gtagaagaga ttttagaaga ctattatatc atacgaactt
    18601 cttgggtttt cgggatctat ggacacaatt tcgtcttcac gatgcaacgc ctagcggaaa
    18661 cccgcgacaa actgactgta gtaaatgacc agtttgggcg accaacttgg acacgtactt
    18721 tggcagaatt tatggtatat gtcatcgaag aaaaagcacc atttggtatt tatcatttgt
    18781 cgaacgaaaa tagctgttct tggtatgaat tcgcaaaaga aatattgaaa gatacagatg
    18841 tagaagttgc acctgttacg tcagaagaat ttccgcaaaa agcaacacgt ccgcagtatt
    18901 cagtcatgag cctgaaaaag acagaagagt taggatttgt cataccaact tggcaagaag
    18961 cacttgcgca aatgctggaa gctgccaaaa aaatgaataa gtaatttcct tttatcaaat
    19021 agaatttgga gcatgatatg gaaaaacaaa caagaagtgc acgacgaaaa gaaaagccaa
    19081 aagcgaacca ggcagattcg aatttggtgc ttgacctgtt tctaaaactc cgctatctta
    19141 ttggcgtttt agtcatcgtg gtcatcgtta cttttaattt aaacggaagt tcacttggtg
    19201 tttgggataa atatgtgtcg caaagagacg atgggaaaaa aactgatgtg atttttggac
    19261 agaatcggga agtgcggtca gacgaatggc tggtgcagac acctttttac ctctcacaag
    19321 ctgaagagga ctttccgttg gtgaacgagg attattcatt aaacggacaa aacatgatca
    19381 ttgcgtataa ttctcctgta aaggatatta cggttatcgg aaaaccattc aactggggat
    19441 tctttttcct cggaagagat cgaggacttt ccttttattg ggcgatgaaa ttagtcgtca
    19501 tggtcttgct tggattcgaa gtactgatga tattaacgaa gagaaaaaaa gctctatcgc
    19561 taatcggtgc atttgcgctg acattttctc cagcagtcca gtggtggttc atgcaacatg
    19621 tgggcgattt gattttcttt actttgggac tgatgactgc tttttatcat tatttctaca
    19681 atcatgataa aaaatggcta cgaacgttga tgatgctttt ggtggttatt ttcggtattg
    19741 ggtttatttt agtgatttac ccagcccatc aagtgatgct ggcgtacttg ctgatttttt
    19801 attttgttgg cctgcttatt tactatagca agaaagtttc atgggatctg ttggatgtag
    19861 tgttgattgg aggggcagtt ttatttattg gcggagtcat ggttcatttc tggctaacgt
    19921 caaaagacgc attgttagct tctttgaata ccttatatcc aggaaaacgg gtaagtactg
    19981 gaggaaagtg gacgattggc gaattttttt ctttccttac aaattggaaa attccttttg
    20041 aagatatcaa ctattcgaat aactctgaag tggccctgtt ctatcacttt tttccaactg
    20101 tattcctagc aagtccattt gtgttatttg gcaaaaaaga tagcgaacaa aagttactgg
    20161 gacgtatttt gatgattttt tgtctgtttt ctatcttttg gattaccgtt gggttgccaa
    20221 aatggctagc agaaatcact ctgctgtctt atgttcctcc attaagagcc atccttactt
    20281 ttagttttgc ggcttgttta ttatcgatct ggtttatcgc ttatatttgg agagaagatg
    20341 ttattcctat ttggtataaa ttcattttgt tggcagtaaa tgcatggtct tatttctatg
    20401 cgattacgca ttcgaaaatg gataattatt tttcggattt tgaaaccatc gcagttgctg
    20461 tgttgggaac agtgatttta gcttttgcat tatttggctg gagacgcttg ttttacctcg
    20521 cttttgccgc cttagttatc gtatctggct ttttcgtcaa tccagtcgta gaaggtacag
    20581 gagcgatttt tgaaaaaaca ctggcaaaag aaatccaaaa aatcgatcaa caagatcctg
    20641 gtaaagtttg gttgtctgaa gatgaattat ataacttcac cccaacattg ggagtgaaat
    20701 ccttcaatac agtacgcttt tatccagata tagctgcatg gaagaaaatc gatccgaaag
    20761 aagaatacga gaagtattat aatcggtatg ctcatacaag agcttttatc gctaaggaag
    20821 atacaaagtt cattttagat cgtccagata tgttttcggt cacgttgaat tttgaagacg
    20881 cagaaaaatt agggatcgac tatgtgatca gtaaacgacc attaactgat tacaaccaat
    20941 tatatgaagc gcagttcacg cagctatatg gaccggataa agacggttat atgattttta
    21001 aagtagaata tccggattat ccagatgtac cacaaacaca aactcaggaa aatcagctag
    21061 tcgtacagta ataggctagc tgagaataat cctgaaatgt tagtagaaag gagagtggag
    21121 ataattcaag caatcgtaca aaaatttgtc aacaaaatga gtgtcggttt cttgcttttg
    21181 ttactgacgc tagtcttttt tgtttggaca gcagggttag gttttccagc aactttgttt
    21241 atatataaca atagttcatt acttatcgct tttagtgtgg ctattttgct cattttaaat
    21301 gtttataaag caaaaggcgt cgattttctt tatctaggtg ttgcgcttgc ccttttcatt
    21361 ttcttttctt actttcggga attaagaaat gagtctttat ttggagactt actgtttcct
    21421 ttgattattt tattttgttt agtaattaaa tggatcgagt ggacaaaatg ggatcgcggt
    21481 atttatctga ttgtcttttc ggtcttcctc ggaatcacgt tatatcgtgt atttacagag
    21541 atccaagtac cagaaggaca aagcatctgg gctccaggca attcgctttc tgatatttgg
    21601 atcaatacga atataatcgg aagttctttg atgactcttg ctttattgat cagtggattt
    21661 gccagttctt ttgaacgatg gtatgtacga atcatcggtg cagtagcagt tgttgcagct
    21721 tttctcggga tttgggtctg ccaatctaga ggtgcattag tcgcgttgat tgtttttgcg
    21781 atattggatc ttttacctaa atcgttgatg cgtgtcattc gtgcgccatt tattgcttac
    21841 acggttacga ttcttttagc tttgccgatt tcttatctgg cggctgtctc tgaaaaagtc
    21901 aatttgttta ccggtcgaga agatatttgg cataaattct atcagacgct aggagaaaaa
    21961 tctgaacaaa ttttactagg aatgaaaaca tttatcttcc aacgaggaaa ccaattttta
    22021 ggaaaccaca atagttataa ttctattttg aatatctatg gattgatagg atttgggatt
    22081 gctgctttgc tgcttatcct ttttattggt cgcttaacgt tgaaagcaga tttatccaac
    22141 gggcagatga cttttatctg ggcctttttt gcggtgatga tgcagtcatt tatggaggat
    22201 acattgactt catacccatg ggttccgatt atttatatcc tgttggctat ggccgttcat
    22261 cgatatgatg aaccagaagt tgcggaggaa ttctatgtta tggaagaggc atatgacgat
    22321 atgacttatg atgatacatc ttatgaggaa caaccagata gcgctcattc ttccaggatc
    22381 caacgctatc attgataggc acaaaatacg agagaggctg tgacaaccgt cgcagccttt
    22441 tttggtttat tgcaattgtc tagcatcttc tgtaaaatag aaacgttaga gaaaatcttg
    22501 tatcatagaa aattaagaaa gaaataagaa cgcagctcgg taccaaaaat atggtaaaat
    22561 ggcacttgac tgtaattcac tgaaaatcgc ttggaggaac acaaatgttt aaagatttgt
    22621 atttatttat gaaggacatt tatcagaaca gaaaattatt gctacagttt tcgatcaatg
    22681 acttcaaatc aagatatgca ggatcgtttt taggaatctt atgggcgttt atcaatccgc
    22741 tatttactgt attgatttat tggctggtat ttggtgttgg actgaaagca caaatgatcg
    22801 atgcaaaata cccatttatc gtatatttag ttaccgggat gattccctgg ttcttcttct
    22861 cagatgcatt tgcatcaact acggttgttt ttcgggaata tacttattta gtaaagaaag
    22921 tcgtattcaa tattcgtatc ttgcccacga cgaaaatcct atccaatctg tacacccact
    22981 tattcttcat tctgattggc atagctgtcg cgcttataaa tggtatatat ccttcagcaa
    23041 tgtctttaca gctgatttat tatttatttt gcatgtgtgc tttcttgaca ggattgactt
    23101 ggctgacggc atcgatccaa ccatttttac ctgatatcat gcaattaatt acgatcgtgc
    23161 tgcagttgat catgtggaca ttaccgatat tatggagccc taatcagttt gattcaggtc
    23221 ttgtaagcat attgaaatta aatccgctgt actatgttgt tcaaggttat cgtgagtcat
    23281 ttttaagtga agcttggttc tgggagcatg gaacatatac tttgtatttc tgggttttca
    23341 cgttagtaat gttcgtaatc ggctctgcag tgttccgtag attgaaacca catttttcag
    23401 atgtgctgta aggagaatag ctatgacaga atatgcaatt agactaaaag atatcacaaa
    23461 atcttataat atgtacgcaa aaccttcgga tcgatttcgt gaagccctca atccttttaa
    23521 gaaatcctat catgatgtat tttatgcgtt gaaaaacgtg aatgtagaaa taaaaaaagg
    23581 tgagatgatc ggtttcgtgg gagaaaacgg atcgggaaaa tctacaatgt tgaaaatcat
    23641 tacgggagta ctgacaccaa catccggcac gatggaaatc gaaggaaaaa tctcagcttt
    23701 gctagaacta ggatcaggat tcaatcctga gtattctggc tatgaaaata tctatttaaa
    23761 tggaatggtt ctaggatttt caagagaaga agtcgatgaa atggtcgatg atatcattga
    23821 atttgctgac atcggagacc atatttatca acctgtaaaa acttattcaa gcgggatgtt
    23881 cgttcgtcta gcttttgctg tagcaatcaa tgtgaatccc gatattttga ttgtggatga
    23941 agcattggca gtaggcgatc ttgaatttca actaaaatgt atggaaaaat ttacagaaat
    24001 caagaactcg ggaaagacga ttttattcgt ttctcatgat atcagttcga ttcgtcgttt
    24061 ttgtgatcgt tcctattggt tgaaaaacgg agaagtcgtc gaatatggcg atacgatgga
    24121 agtaacgacg aattatgaaa actttttaaa gaaaaaatca gttaaaacag tggatcggaa
    24181 taaaaaccaa gtagaacggt tcgccgctcc agatatcgtc gacgtgacga aagcggaatt
    24241 gttggataaa gacaaaaatc cggtggatat gctggaacaa ggagaaaaac tatacgttaa
    24301 agtaacttat gaagtaaaag atgatacaat caaaaatccg gttttaggaa ttgcgctacg
    24361 aacagtcgac aatttttatg tatgcggact taatactcta ctagatggta tcaagattcc
    24421 ttggaaaaaa ggagagaata tcttttatct ggagtatgaa aagttaggtt tgttagctgg
    24481 tgaatattat tttgacgttg cggtctttga agaaaatgca acggttcctt tggtatataa
    24541 gacaaagtac atgaacttgt ttgtcagcgg ttcttatatc ggagaaggca tcgtggtctt
    24601 agaccataaa tgggaagaag ggactcatag caatgaaata tgattttgaa atggacctag
    24661 atgaacaaag cagtgtggga aaaattgccg cacagatcaa accaggaagt aaagttttag
    24721 agtttggtcc cggaaacggc cgattaacga aacatttaat cggagcaaaa caatgtgaag
    24781 tgagcattgt tgagttagat aaggaattgt tcgattttgt cagcgaattt gctcaagatg
    24841 gattttatgg tgatatcgaa agttttgagt gggcaaacta ttatgctgga cagacatttg
    24901 attacgtctt gtttgcggat gttttagaac atttggtaga tcctggaaaa acattgaaga
    24961 aagtcagaga atttttgaat gaagaaggag agatcctgat tacattccct aacttggcgc
    25021 ataattctgt catgatcgat ttgttcaata accaattgcc gtgggcttct tatggtctcc
    25081 tagatgaaac ccataattct ttttatactc atgatggctt ccaaaaagtt tttgaaaaag
    25141 cgggattatt cattaatatt gaagattatt tgtacttagc tgtaggtgat acagaattaa
    25201 aatcaactta tgaagaattg ccggaagctg tacgctatga tttcaaaatg cgaccatttg
    25261 gtgaagtcta ccaatacttt ttctcattga tgaaacatcc agtagcacaa agttccattg
    25321 cagagccaca aaattctaat tatgtaaaag tgttagaagt aacccagcaa acgaaacaag
    25381 atgaaacaat ccaacaaata ccattcaata atttcacagg ggaaaatgag acactaagtt
    25441 tccccgtatc agaaacaaca gaaagaatgg tgtttcgctt cgcgcaacaa cctagcttta
    25501 tcgaattcag cgcagaagct gccggagaaa aactaacatt tatcgattcg aatgctgtag
    25561 tcaaaacagt aaatgattgt tatttgtttg atgggaaaga attaccagag ttcgttctaa
    25621 cggatatttc tgggaaagaa gtgactatcc attgtcacta tcgctttatc ggggaattga
    25681 caccaacaat gaaagaattg ttggaaacga ttcgtcccat ggctgaagtc acaaagcagc
    25741 tttctgagaa aaatgacgaa ttagaaagag aaaatcatca tcttcttgag gaaaatacaa
    25801 gactagacca tacgttaaaa acaaccacaa atcgctattg cacattgttg gaaagtgatg
    25861 aatggacaat aaaacataga ctaggacgac gtaagaaaga aacttcgaaa aaaatccaag
    25921 aaaaagaact ttctatatgt attgacgaga aaatctggga tgcagaaaca aaaatattaa
    25981 aaatcatcgg atggggaatc gctaattcgg atcgccagcc cttgtcctat aagttatcag
    26041 ctgaccaatc gccatttttc gaagcaattc ctgtttcacg agaagaagtc aatcaagcag
    26101 aacagctcgc aaaaggaaca gaagcgggat ttgagttacg aatcctttgc gagcaagaac
    26161 gttcattctt agtggaagca gtagctcaaa atggccaatc atggatcatt gaaatgtaaa
    26221 taatgattaa cttataaaga taaggagtaa acgaatcgtg caaaaaaaag aaattgccgt
    26281 atttatcgat caaatcactc gtgaacgggc cactggcgat ttgcttattg tgggctgggc
    26341 aattgatgaa gtgaccaaag aaatccctac gataaaggta gaaaaagaaa atgtgattgc
    26401 tgaagcaaca catgtcgtaa gattggatat caatcattta tataatcttg atgttaagac
    26461 ccaaagcggt tttaaggtac gtttatcagg gaaaatgaga ggaaaagcca tcttagactt
    26521 ccaaacagcg agacatcaaa atggcatagc tgtgaaatta aacggtaaat acccttatga
    26581 tgacggcatt gaatcttcat gggaaagaaa aaaaagatta ctgaaaaaag ggataaatta
    26641 tgcaaggact catggcgtaa aaaaagcgat ccgtcgggtc aaattggaat tgaagccagg
    26701 atcgatcgac tatgcagagt ggatcagtcg tcatgagaaa ccagatttga aagagcaaag
    26761 gaaagaagca caaggatttg cgtaccgtcc attgatttcc atacttatgc ctgtctataa
    26821 cgttgaaatc aaatggctcg aaaaatgcat tgattcggta ctggatcaaa cgtatgatca
    26881 ttgggaacta tgtatcagtg atgatgcttc tactgatcca gctatccgaa agtgtctgga
    26941 atcctatcaa gcaaaagatg atagaatcaa agtcgttttc cgaaaagaaa atggacacat
    27001 cagtctggca actaattctg cattggaaat agcagaagga gaatttatag cactcttaga
    27061 taatgatgat gaattaccgc catttgcatt atatgaagtg gcaaaagtgc taaacgtaca
    27121 tccagaactg gatgtgatct acagtgacga agataaaatc gatgcagatg gcaatcgctt
    27181 tgatccgcat ttcaaagcag actggtcgcc tgatacattg atgggaaata attatatctc
    27241 tcacttaggc gtatatcgaa caagcattgt taaagaactt ggcggtttcc gtaaaggcta
    27301 tgaaggttct caagattatg atttggtcct tcgtgtgaca gaacaaatcc ctgcagatca
    27361 tatttaccac attgaccgag tcttgtatca ttggcggacg atcccaggat caactgcaag
    27421 taatggcgaa gccaaaagtt atatctatga ttccggagtg aaagcattga cagatgccct
    27481 gtctagaaga aacatcaaag gaagcgtacg tcctggcaga atttctggat tttatgagat
    27541 tgcttatgac gtcttgcaag aggatttggt cagtgtgatc attccaacga agaatggcta
    27601 tgaagatcta aaaacttgtg tggattcgat catcgaaaaa acgtcttatt caaattatga
    27661 aatcattatt gcggataacg gaagtacaga tccaaaaatg caagaattat ttgcagaata
    27721 caagcatcag ctgaaagacc gctttatcgt agaattgatt gacattccat tcaattattc
    27781 tcgaattaac aatttagcag cagaaaaagc aaacggcaaa tactttttat tcttgaataa
    27841 cgatacagaa gtgattgagc cagattggat gacggtgatg gtttcttatg cacagtttga
    27901 tcgaatcggc tgtgtaggag caaaactgtt ttatccagat gatacaacgc agcatgcagg
    27961 agtattatta ggaatcggtg gtgttgcagg gcatgcattg aataactatg atcgaacaca
    28021 ttgtggttat ttcggccgat tggtcattga cgtcaattac ttagcagtca ctgcagcttg
    28081 tatgatggtc aaggcagctg acttcaatgc tgttaacggg tttgatgaaa cattagaagt
    28141 agcattcaac gatgttgact tgtgcttgaa agtttatgaa ctgggtcgtt acaatgtcta
    28201 tgcacatcaa gcagaattgt accatttcga atctaaatca agaggttatg aagatactcc
    28261 agaaaaacaa agacgttttg ctggtgaaat caaaaaaatg caagataaat ggccagcata
    28321 tattgcgcat gatccatttt acaatgataa tttaacaaaa gaaggaatcg gcgacttttc
    28381 gttgagacca gattaaacta taataaaaag ctgttatgtt gtgattcgct cgcacataac
    28441 ggctttttat agttttttgt caaaaaaagg cagaaggtgc cgactcaaaa aaataagtcg
    28501 aggttgggac agaagtgttt aactccgaga aataagaaga aattcacgaa aattgctttt
    28561 caaatttttg tgaatttcgg cttatttaag aagaagttgc ttctgctccc accgtttatt
    28621 cgtttttaaa gaatgagaca aaagtgtttc ttacttttgt cccacgctct tattcatgct
    28681 cagagcgcca atagatacaa aatcatagcc aaagtttcac gaaccctagc tgtcaaagaa
    28741 tcgatatttt attttaatat aggctatttt aaaataaatg gtttaaggta ttcctctaca
    28801 aaagccactc tatcaattat catgggttta aaataagaag tgatggagat aaccagattg
    28861 ttcttagcgt caatataaag aacatttcca ctattaccaa ttgctgaata tatttcgttc
    28921 tcagaatcta atacccacca taaatagcca tatctcatat tttgtaagtc ttctaaagca
    28981 ctggttctag gtttgaacat ctcgtttatc cacatgggtg gtacgatttg ttttccttta
    29041 tacatccctt tatttaggca taatacacca atttttgcca gatcacttgc cgataaacac
    29101 aaaccatatc ctgccgtccc gatatttagt ggatcacaaa accagacatt ggttttagga
    29161 gacttatcca ttgtaaactg cttatgttct tcagctgttt cagcataata attgacatgg
    29221 tttggtatcc ctataggatc aaatagatac ttattggcaa aatcaacagt tgttagctta
    29281 cttgtctgat acaacaagcc agttaatatg tgtaaacaga cagtttgata gttgaattcg
    29341 ccggttatac cttttcttcc tcccaataaa tctagtgatg tttttgtcca atcttcactt
    29401 gaacagactt ttgtccatgg atcgccttta catttgtagg gtgctttcat tgttagcaag
    29461 tgcttgattg taatagcgta aatagttttc tccccgcgtt tcactttata ctctggaaaa
    29521 taatctaata ctttatcatc gatacttttg attaatcctt gctctaaagc aatgccaatc
    29581 agcaaagata caatactttt cgttgctgac atgatatgac aagtatcgtc ttttttataa
    29641 ccattccatt catctgtata aacttctacc ccatccttat atgcgacaat ttgacatata
    29701 tttggttgtc gaaaaccaat aaaatcatgc aactcttgtt tattcataag atattacctc
    29761 actttcttct ataagcataa tgtcattaaa aatcgatttc aattttttcg attcttcacg
    29821 taatacaggt attcttatgg actagacagc gctttgggaa gatggattct ctgaaaaata
    29881 taaaggttca ctttatgagt ggataaaaaa agcagcaaat acttcgactt ctttaattta
    29941 ccaacattag tgaaaatcga ttcatctgga gatttgtaat ttagagtcct tgtcttgatt
    30001 agaggtgtat aaagcttttt attagacatt tttaaattcc attacaattt gcttataaac
    30061 taggttttta aaggtgacta tggtaagata atagagatat tattttgtga ttaggagtgt
    30121 atatatgaat aaaaatggag aatggagtgc tgcacgacgg atattcatta tattaatgga
    30181 tgttattgta tacaatgcag caatatatag tagtttctta ctgaagtttc gtggagagat
    30241 cccattacga aatttagaaa ctttccaaca ctcagcaatt attatttcag tgatttttat
    30301 tgcgttgaac attttgttgg gtgcatatgt tttttataat cgaatgatca gtgacatcgt
    30361 gtttgtcaca gtcatcggtc aggtattgat gacactaggg atcatggtag tgacttttgc
    30421 gggccgctgg tttgcttttc cacgtcttgt tatcttactt tcatttgttc tcggtacgct
    30481 tttcttaagt atctatcgta ttctcgtata taaactttat ttacgcgtaa gtcgagataa
    30541 aaaggtcgtc attattgggt tagaaaagga tgtcgcacca gcaatccaaa attttggttc
    30601 aaagaaaaac aacaaacata aagtcgaagc agtcattctt gataactatt atgcaaacat
    30661 caaaaatatg attaaagaca tcgatattgt ctatcttgct tctcatatcg aagaaactga
    30721 aaaaatcaaa atctatcaat tagttactaa aaatgaaaaa aaattgtttt tgaatacaac
    30781 atttgaaaac ttgacattga tcaatccaaa tattatgaat ttcgaagatg aaagtattat
    30841 tgaagcatct ggatttagaa tccaacctga atatgaacta ttcaaacgtt tgtttgatat
    30901 catcgtttca cttgttttat tagttattgc ttcaccattt atgttattca cagcaatttt
    30961 agtcaaagtg acatcaccgg gaccaatcat ctataaacaa gtgcggatca caaaaaatca
    31021 aaaggaattt tcaatctata aattccgctc gatgaccgcc acagccgaag caaaatctgg
    31081 accagtttta gctaagagca atgatgcacg agtaacgcct gtaggtaaat ttattcgagc
    31141 tgtccgcttt gatgaattac cacaaatctt caatgtgctg aaaggcgata tgtcgatcgt
    31201 aggaccacgt cctgaacgtc ctttcttcgt cgatcagttc aatgcagaaa acccttatta
    31261 ctacttacgg cataatgttc gtgctggtat cactgggtat gcacaagtct atggaaaata
    31321 tgtctctgat tacaacagta aattacgttt tgatttactt tatatcaaaa aatattcatt
    31381 gatgcttgat ttaaagatct tgctgcaaac catcaagatc ctatttgata aaatgtcctc
    31441 tcaaggactt gaagaagacg aaaatgtgat ctcattggca gaaattgctt ttccgaatga
    31501 aaaaatcttt cgctagttgg gaggcgtaaa aatgaacaat ccgaaattca gcataatcgt
    31561 accagtatac aatgcagaag caactattgg tcgaacgatt gatattctaa aagaattgga
    31621 ttatgaagag tacgaagtcg ttttagtaaa tgatggatca acagatcaaa cacaggaaat
    31681 cattcaagaa ctgattgaaa atgatgaacg ttttgaattg attactacga ccaatcaagg
    31741 gcccggatta gcaagaaacg aaggaataag acagtcaaaa ggagaatatc tcttattctt
    31801 cgatgcagat gacacgccag taaaaacgat cttgtcagat tatgatcatt tgctagaaaa
    31861 gagtccaaaa gcggatcttg ttgttagttc gtttgttttt cgtacaatgg acgagggaag
    31921 aatcgtttcc gaaaaagaaa acttagttac agaatgtcac tatctaactc acgaagagtt
    31981 tataaaagac atgtatcagt taatgaatga acagttgatg tatgttgtat ggaacaaatg
    32041 ctatcgtagc gatattctga aaaataacca aattctattt aaaacttata atagttgtga
    32101 ggatcgaata tttaacttac attattatga atactgtcat caagtgatca tgaacccaaa
    32161 agtagaatat atttatgaat ttgaaggtgg gaaggggatc actaatcaat accgacctaa
    32221 caagttcgaa acatttaaag aattctattc attagcaaat actgtaacag acgatattaa
    32281 taaagacggt atggcggcgc ttcttttaaa agggacaact tcagttattt tttctattta
    32341 tagtacctct gcaagaactt caaaagagaa gaaagacgaa gcaaagcaga tattgagtga
    32401 tccagtgatc cgagaagcca agcaaattgc taagacggat tcttcagcaa aaaaaataac
    32461 aaagttgctt tacaatatgc cagctccact atttttatct gctgtaaaag ttggaagctt
    32521 cgttgaagtg aaaatgccag ggttaatggc tgctttgaag cgagtatatt agttaatgga
    32581 ggaaaatata atgaaaagaa ttttgcattt tcagggacga atgggtttag gtggagcaga
    32641 gtctttcatg atgaatttat atcgtaaaat tgatcgtaca aactatcaat ttgatttttt
    32701 gatttatgaa gattatgcag atgttcaaga ctatcattca gagattgaaa gattaggagg
    32761 ccgtattttt gttgtaccta atcctaagaa aaatattttt aaatatttaa tagaagtgaa
    32821 taagttacta aaaaaagaat ctttttctat agttcacaat caggtttatt ttggcggggg
    32881 aattaattta tggttagcca aaaagaatgg aatcagacag agaattgctc atagccatgc
    32941 aactgaagat ggaaaatctc aaaatattgt aatgaatgta ttgcgtaaat ttttaaccaa
    33001 attgttgtta caaaatgcaa cagactacct tgccgttagt caagaagctg gtgaaagtct
    33061 gtttcaaaac cacccctttg agatagttca taatggtatt gatttagagc tttatagtaa
    33121 aaattcagaa gctaaggtta ataagagaaa agaattggat atctcaatga gtacttttgt
    33181 tgtgggaaac attggacgat tagagaaaca aaaaaatcaa tcttatttgt tagagatttt
    33241 tttagaaata ctaaaaatga attctgattc ttacttgatt ttagtaggtg aagggtcact
    33301 tagaaataaa ctgaaagcca aagcagtaga attggatata gagaaaagag taaaattttt
    33361 aggagaaaga aacgatattc ccgagttatt atctgtgatg gatgtttttg taatgccttc
    33421 tctttatgaa ggtctgccaa tggttggatt agaagcacaa gcagcaaaat taaaattagt
    33481 cttagcagat actatttcca aagatacaaa gttaacaaat aatgtggatt ttgtatcatt
    33541 agatgaaaca ccacaagaat gggcaaaaaa agtaataaaa gagccgtatg ctaataaaat
    33601 aacacaagaa ctaaagagtt atgatgtttc gtacactact gaacaaatgg agagtatata
    33661 tagtagagat cattaaaaat gttcaagata ttttaggagt gattattttt gaaaatatgt
    33721 tatttgatat tggcatacaa tgatattggc aacttaaaga gattggtcaa tcgattgaac
    33781 gaaaatgcag tttttattat tcatatagat aaaaaaactg atatagaacc atttattgaa
    33841 gcatttaaat catatgataa tgttgaattt ttatgtaata aaaaccgtgt taaaatttat
    33901 tggggtggtt tttccattgt acaagcagaa atcaatttag taaagagagc attacaaaat
    33961 gagaaatatt taaaatatgt attattatct ggtgctgatt atccaatcaa agataatgag
    34021 tatatttata attattttaa aaagaatagt tcagtggagt ttattcgtgg aatagatcta
    34081 gatcaaatta aacataaaga attttattat aaacatatag atgtatatca aaaacatgat
    34141 tatcctagaa ttaataggaa taacacaact gcatttaaaa tttttagagc tataattaat
    34201 cgttgtctga ggatgattaa acttccccca aaaattagac accataaatt tgatttgtat
    34261 catggttctc aatggtgggc tttgtcgaaa gaatgcctaa cagaattaat acaaatgtat
    34321 gaacaaaatc aacaagacta cctgaatttt aaaataggta tgtttgcccc agatgaaaaa
    34381 ttttttcata cacttttttt taattcctcg ttcaaaaata aaaatgttat tggaggacca
    34441 gatttaccac ttgaattgaa aaatattgaa gaaactactt tacaaacttc gaaattagca
    34501 aatattcata ttattgatcc ttccatgaac aaatggtttg atgaaaggga tatagatcaa
    34561 ataaaagaaa gtgaaaaatt atttgtaaga aaagttaaat cggattactc tgataaacta
    34621 ttaaatagaa ttgataaaga aattttgaaa gttggaaata tagatgaata gaatagtacc
    34681 tgttttagag aagagtttat taagtcgaga tgcgtttcga gtaataaatt taatcatttg
    34741 tctgctttat tttatgccta gtacttttaa tgtaactaat atcccgatga aaatagcttt
    34801 tgcttggggt ggaattatag ttttgtatga ggtttttata aagaaaaatt tttttaaaat
    34861 gcgtttttct tggctactga ttggagcaat gattagtttt atcttaacta taatcattaa
    34921 cagagaaaat tttttaattc catctattta taattttgga tatttgctga ttactttgat
    34981 agtaattttt cctgcagact tttcacaaag tgaagaggaa aaaaagaaaa aaatggtgta
    35041 ttttaataat gttttcatta tcataatttt tttagctgcg ctaatatcta tctatcaatt
    35101 tatttttttg atttcttatc atgtcccaac aggaacgcca ggattacttg caagacaagg
    35161 ttttattgaa catcggttat ttggtgtcta tacaagtcca aatgttggag ctatgtttgg
    35221 atatacttct attattttaa gtttaattag tgtattaatt tcaaaaataa caaaaacgat
    35281 tcgtgttttt tatatgatga attttatcgt acaaatattt tatattacac tttcttcgtc
    35341 tagaggtgcc caggtagtaa tttgttcttt tttagtatta tttcttttgt tgtttgccaa
    35401 ttcaaaattt agaaagagca ttagagaagt actacctgta aaaatcaaat ggatggtaag
    35461 catattatta gttctattgg tatatttttc ttttggtact aattatgcta aggatttttt
    35521 agcaacaatt cctgtatcga ttgatagaaa agtagaagtt atagagtcaa atgaatcaga
    35581 taaaactcca cttccagaaa aagaagtaat attacagcat tctagtgata actccgaaat
    35641 ttcagctggt cgttttacga tttggaaagc tgcgcttaaa gttataagac caactttgtt
    35701 atttggttat ggagagaccg atttctatgg gaatagcgat gcaaaatttt tgaaaaaagt
    35761 agatcttgat actacagatg tgaatgaact aaaaagagca catggaaaca tgcataatgg
    35821 atttcttcaa gtacttgtat caagtggtat aattgctttt atatgtatct atgctttcta
    35881 tgtgttaaat atctttagtt tactcaaaac attttttaga agtaaagtga attatgcgtt
    35941 tcttggtatt gttttaattt ttatactatc tatttttata gatgaaatgg tggaggctca
    36001 tgtacttttc aataagcgcg atgtcatttc aattgttttc tggtattatt tgggaagcat
    36061 ttcttacttg tatttaaaaa aggagaatta atatgaagac agctttcatt gcttggactc
    36121 ctttacattt gattaatata ttaaatgttt atcaaaattt ttaccgagaa tcaaaaggag
    36181 atttatttat ttattctgaa tttgaaaacg ctagaagata tagtgagcgt ataaaagaat
    36241 tagggctatt taatgatgtt gtttttgtga atcaccggaa aatcggttct tcctttaaac
    36301 gtaaaattaa cttgttaact aaccataatc aatttttctc ggatgattta tccgtttatg
    36361 atgaaatttt tatacagggg gaaaattatt tcacaaaaat acttttctcc aatgtttaca
    36421 aaaaaaatcc agatgtaaaa ctaaattata tagaagatgg tttgggtact tatttgaaga
    36481 agagtatatt ttcaacaagt aaaaacgttc aaagagtttt taaaatatta aataaaaata
    36541 gcatcttcaa aaaaaaaatt gacgattatt ttgtctattg tccggaattg ttactttatg
    36601 atgtaaaaaa tataaaaaaa attcccaaaa tagatgaaaa cagtaaattg atgaaaaaca
    36661 tacaatcaat ctttgggata gaagaagttt ttgtcggaga taaatgtatc ttttttgatc
    36721 aacctttatt atctgatggt tatggcatag atgaaacaga tattttcgct aaagtaaata
    36781 gtatattttc tggaaaaaaa gaaactttaa ttaagcttca tcctagagca aggacaaatc
    36841 gttatggtga aaaaaacata ttgaattcca gtttaccgtt tgaagtcttg ctacttgaaa
    36901 atgacttctc ttcaacactt ttgattagtc ctatatctac aatttctttt tcaccatcta
    36961 tgatgtttga aaagacgatc aactctatta tgttggctaa attaatttta aatgaatata
    37021 cagcattatc tgaagataaa aaagaaacat tgacaaatat tgttcttttc tgtgaacatt
    37081 ataaccgtct atcagaagaa aaaattttac tcccagaaaa ctgggcagaa ttaagaaagt
    37141 tggtagagta tgggattatt taacacactt tccaagtcaa aattagttaa gtccagtttc
    37201 tggtatacta tagggaattt ttttattaag ggaatttcat tcataactat tcctattttt
    37261 gtaaatatta ctactcaagc agaatatggt ttgattaata atttcacttc atttgtatca
    37321 attttttcga ttcttgttgg gctaagccta aattcggcaa ttaataatgc aagttttgac
    37381 tataaagatg acataaatga atttatgtca tcggtacttt ctctagctac actttcgtta
    37441 attgtgttct tattaatagg gaacatatat tttttgtttt caaatgcttt ttttgatatg
    37501 aaccaacttg tatttaattt gatgttatta caaagttttg ctagttttgt aattagttat
    37561 atccagtctt attttacgat taactttaac cattttaaat atttgatttt atcttttata
    37621 tctacttttt tgaatattgc aatctctgta gctttgatgc agacattata ttttgatgat
    37681 aaatatgttg gcagagtagt cggcggaaca ctgtcatttg tgttgatagg tttagtaatt
    37741 tatttaagaa taatgataaa gggtgctcat ttttttgata aaaaacagtg gagatacgct
    37801 ttaaaaatct ctttgccgct aatcccacat tccttagcaa atatagtttt agcacaattc
    37861 gatagaatta tgattaattc ttatttaggt cctgctgcgg ggggaatata ttcctacatt
    37921 agtaacatag gtattattct aagcgtaata tggatgtcta ctaataacgc ctgggttcca
    37981 tggttctatg gtgagatgag taaaaataat gaaaaaaaag tgaagaaagt tagtaactat
    38041 tatttagtag tctttacttt gattacgatg attgtaatgg tagtttcgat tgatttagca
    38101 aaatttatgg ctccgaaaga ctattattct gggttatctt tagtggttcc gataacctta
    38161 ggctatttct ttcagttttt atatagttta ccagttaatg ctgaatttta tgagaaaaaa
    38221 acatcgttta ttgcgattgg tacagtagca agtgctatag ttaatatact attaaacatg
    38281 attttcatac ctaggttagg aataattgct gctggataca caaccgtatt tacgtatttt
    38341 ttacttttta tttttcatta taatatcgct aaaaaaatta cgaataaaca attatttgac
    38401 acaaaaaaaa tatgtatcat aataattttt gtatcaattt tttctttgat acttcatatt
    38461 ttaatcaatc aaatgttgat tcgttacgtt ttgacatcat tattgataat tagtttagtt
    38521 atatattttt taaagattaa gaatcgaatt atagatgact ataattcata ggaggagata
    38581 taagtgttta aaggaaaaac aatattagta actggcggaa ctggttccat tggaagtgaa
    38641 attgttcgtc agcttttaaa atatgaacca aaagctatca gaatttatag tagaggtgaa
    38701 gaaaaacaat tttatatgca acaagagctc tcaaatcaca ctaatttgag atttttaatt
    38761 ggtgatatca gagactctca aaggattgat tatgcatgtc gtgatgtgga tattattttt
    38821 catgctgccg caatgaaaca cgttccagca agtgaatata atcctatgga agctgtaaaa
    38881 acaaatgtga tgggaactca aaatgtaata gatgcagcga tcaaaaataa tgtaaaacat
    38941 tttgtaggta tcagtacaga taaagtagtg agtcctacta acaccatggg tgcgacaaag
    39001 ttattgagtg aaaaattaat aatagctgcc aatcagtata aaggaaaaac agaaacaata
    39061 ttttcttgtg taaggtttgg aaatgtcatg gggtcaagtg gatctgttat tcctcttttt
    39121 agtaaacaaa taaaaaacaa taaagatatt actttgacag atgttgatat gacacgattt
    39181 atgatgtcaa taccggatgc tgcaagatta gttctgtctg ctgctatgaa ttcaaaattt
    39241 ggagaaacat atgtcttaaa aatgccggca atcaatattt taactttagc tcaatcatta
    39301 ataaaaatat ataatgatac acatgatgaa aaatatagag gtgaaataaa agttgtaggt
    39361 atcaggccag gagaaaaatt atatgaagag ctaatgacta cagacgaaat tgaacgctca
    39421 tatgaaaatg ataaaatgta tatcattcca ccacactatc atgctactga cgaactgtac
    39481 aaagattatg aaaaaattaa aatcactgca tactcttcag atgcaacaga attaatgagt
    39541 ggacaggaat ttattagaat cgcaaaagag aataatgtcg tgacttggta aggagtttta
    39601 tgatgaaatc agaagtatat gtaattgcag aaattggtgt aaaccataat ggaagtctag
    39661 atttagcttt agaaagtatt gataaagcta aagaatgtgg agctaacgcg gttaaatttc
    39721 aaacatttaa aacgaataag ctagtttcca aaaaggctcc aatggcaaaa tatcagaagg
    39781 ataatcttaa agaggacagt tctcaatttg aaatgctaaa aaaattagaa ttatctcaat
    39841 ctgattttat tagaataaaa gattattgta tacaaaataa aattgatttt ttgtctactc
    39901 cttttgatga agaaagtgca gactttctaa aacaaattgg tgtacaagga tttaaaattg
    39961 gctcaggaga tttaacaaat ttaccttttc ttagaaaaat agatatgtat gggttgccag
    40021 ttttattatc tacaggtatg gctgatattc atgaggtcag tgaggctgta gattgtttca
    40081 tagatagtcc agttactatt ttacattgta cttcaaatta tccggctagt tctgaagata
    40141 ttaatttatt ggcgattaac acattaaaag aaatgttcgg gatacccgtt gggtactccg
    40201 atcattcatt gggatatgat gttggtattt gtgctgttgc gatgggtgct aaggtaattg
    40261 agaaacattt taccttggat aaggaactac ctggtccaga tcataaagct tctttagatc
    40321 ctcaggaatt tgcagatttt gtaaagcata ttagaaacac tgaaatattt ttaggtgatg
    40381 gtgttaagcg agcaatgcct tctgaactta gtacaaaaga agtatcaaga aaaagcctag
    40441 tggtcactga gaatttaaat gtgggagata ttcttacaga gaaatctctt acaataaaac
    40501 gtcctggaaa tggaatagct cctaagtttt tatatcaata tattggcaaa aaagttaaaa
    40561 gaccaataag tgcagatagt gttctgcttg aggaagatgt tgaatgaaaa aaatacttat
    40621 agtaagtgga acacgagctg attatggaat atataaacct gttgctgaga atttactaca
    40681 agataattgg gatgtatcat ttgttgtttc tggaatgcat ttatcccata aatatggatt
    40741 tactaaagaa ttgatcttag ctgacggatt tagaattagt ggagaagttc aaagtttgtt
    40801 tttagagaac aataagggaa acatggctcg gtctgtggca ttagagataa atggtttcac
    40861 aaatatattt gaacaaacta gccctgattt tgtattatta ctaggggata gaggagaaat
    40921 gttggcagct gcgatagctt gtttgtatct tggaatcaaa acagcacata ttcacggagg
    40981 cgaagtgtct ggtactgtag atgaatcaat aagacatgcc ataacgaaaa tagcatctgt
    41041 tcattttgct gctacggaaa aaagcaaaga gcgtttaata aaaatggggg aagattcttg
    41101 gagagttttt cccgtcggag ctcctagaat agatactatt ttaaataata gattacctag
    41161 ttttagagat ataaaagaaa aatatcatct aaaatttgaa aagaaaaaat acgttttgca
    41221 agtatttcat ccagttgtaa ccgaactaga aaatatagaa aaacaaattt tagctttgat
    41281 caattcaatg gaacaacaat ctcaaccaat tatttgcatt ttacctaata gtgatgctgg
    41341 tagcgaaatt atcagaaaaa tgtaccagaa atatttaaaa acggatatta tttatattac
    41401 gaatttagag ccggaggaat acctgactat attaaaagag tgcaaattta tgatcggaaa
    41461 ttcttcttct gggataattg aagctgcatc attcaaaata ccagtaataa atttaggaac
    41521 cagacaaaat ggacgagaac aatctaaaaa tattataaat gcaaatctaa atgaaaatga
    41581 aattgaaact gctttgaata aaatacaaga tcctcagttt ataaaaagct tgaaaaatgt
    41641 taagaacatc tacggtgacg gcaacagttc tagaagaata acaaaaacat tgaatcaaat
    41701 attgaatgat ggggaagata ttaagtggat tcaaaaacaa atagcttatt aaaatatact
    41761 agtgatccat acattattat tggggctggc ggacacgcaa aagtcattat tgaaacctta
    41821 agattagaaa ataagttaat atatggattt tttgatgata agcaaaataa aatgcaagat
    41881 ctgaaatatc ttgggaaaat aaacgaattg aatagctggg cggatagcaa aaaaaaaatc
    41941 tataaattta ttgtagctat tggtgactgt gtagtaagaa aaaagattat aaaaaaattg
    42001 aatatgccaa gtgagcaata tggaatagct gttcatccaa gtgctatagt ggcaaaagaa
    42061 atttcaattg gttatggaac tgttattttt gcgaatgctg taattaaccc agatgctgta
    42121 attggagagc atgctataat aaacacaggt agcattgttg agcatgattg tagaattaat
    42181 aactatgttc atttatctcc aggtgtatgc ttagcgggtg gagtacatgt aggtgtagga
    42241 actcaaatag gaattggttc tcaatgtatc cagatgaaga aaattggatc ttggtgtatg
    42301 ataggcgcag ggagcactat tgttaaggat attccctcac attccttagc ttatggcaat
    42361 ccagctaaaa ttaagaaaga aggaattaat tttgaattct agaattttat tatcaagtcc
    42421 tcatatggga ggaacagaag aaaaatatgt tgctaaggct tttaaagata attggatagc
    42481 gccattaggt gaaaatgtgg ataaatttga agaaagtatt acaaaatata ctggttctaa
    42541 gggcggagct tctgcgttaa gttcaggtac agctgccata catcttgcct taagactgct
    42601 taatgttgga aaaaacgata ttgtctttgc tccttcattt acttttatag ctacagtaaa
    42661 tcctatttta tatcaagggg ctgtaccagt gttcattgac tcagaaccta atacatggaa
    42721 tatgagccct gttgcactag agagcgcatt agaagaagct gaaagaaaca accaattacc
    42781 taaggcaatc atcgtagtga atttatatgg tcaaatggct gattatgaac gaattttagc
    42841 tattgcaaac caatttaatg ttccagtgat agaagatgca gcggaatcgt taggttctac
    42901 ttataaggga atttcgtcag gcttatttgg gacaattggg atctattcat ttaacggtaa
    42961 taagatcatc acaacctcag gtggaggagc tctagtgtct agtgataaaa aatatgttga
    43021 gcattcaaga tttttagcaa cacaagcaag agatcaagca ccgtattatc aacatagcga
    43081 aataggttat aattaccgtc taagcaatat ttctgccggt atcggacgtg gacaaatgga
    43141 agtattgaat gatagagtag ctaaacgaag agaaattttc aatcgatata aggaagcttt
    43201 ttctggaatt gatagaattg aaatgatgcc agaagtggat ggcatggtgc caaatcgttg
    43261 gttgtctact ttaacgatca atgattataa ttctattgat caagtagtta acgtaataaa
    43321 taaactaaat gaaaaaaaca ttgaaacaag agctttgtgg aaaccactac atttgcaacc
    43381 agtatttcaa ggggtaaaaa tgtattacca tgataataat cttagtgtgt cagaaaagtt
    43441 atttaatagc ggcttatgct taccttctgg aagtaatatg acagatagtg aacaagaatt
    43501 tgttattgat gagtttaaaa aaatattcaa gtaggtgtga tattttatga acataggaat
    43561 tattggttta ggttccatgg ggaaaagaag acttagactc attcaagaac actttaatga
    43621 tattttacta tgtggtgttg atactaatga ggcgagacga aaggaagttg aaaaggaatt
    43681 tggaatacca acttataata gtctaacaga ggcaaaaaat ttgtttgaaa taaattcagt
    43741 ttttgtttgt acatccccta taacccatga atttattata aaagaggctt taaatttaga
    43801 ttgtaatatc tttacggaaa tcaatttatt aaatgaatat tatgatgaaa taatcgaagt
    43861 agctaaaaag aaagatagat tactttatct atcttctaca tttttgaaga gaaaagaaat
    43921 tcaatatatt aagtctaaag tgacgcataa taaaaaaatt tcttatcaat accatgtagg
    43981 acagtattta cccgactggc atccttggga agattatcgc aatttttttg tttcaaacaa
    44041 aaaaactaat gggtgtagag aaatattcgc tattgaatta ccttggatta tagatacatt
    44101 tgggaaagtt aaatcgttta ctgtaatgac ccagaaatta tccaatcttg atattgatta
    44161 ttcggacagt ttcactctta ttattgagca tgaaactggt atagttggaa ctttaagtgt
    44221 agatgttgtt tcaagagtag ctaaaagaaa tttaactata attggtgaaa agattttaat
    44281 agaatgggaa ggtaacccgg attctctatt ttcatggaat gaagaaactt ctagtatgac
    44341 agatattcaa ttgtacgatg aggttatgca tgataataat tattcacata ctattattga
    44401 agatgcatat ttagaggaga ttaaagaatt tttcacgctt ttggaaaaga aaatggaacc
    44461 ccaatatact tttgaaaaag ataagtatat tattaactta attaacgaca ttgaggtaaa
    44521 ttaatgatga aagtaatttt ttttgggtta gggtcaattg gaaagagaca tctaaaaaat
    44581 ctaataagct atggaaaaaa gaattctatt aattttgata taacagctta tagtagcagt
    44641 ggaaaaaaag agtttgaaga aataacgtat attcaaaata gagacgattt ggatacacat
    44701 tatgatatag cttttattac aaatccgact tttaagcatc tggaaacact aaatgtaatt
    44761 gttgaaaaag ttgattacat atttttagaa aaacctgctt tttctgagag tattgatctt
    44821 actcaatata attatgatta caaaaatatt tatgttgctg ctccactgag gtataaaaat
    44881 atcatggaag ttgttcgtaa tagtattcaa agtaaaaaag tatattctgt aagagctatt
    44941 tgttctactt atcttcctga ttggagaaaa ggtactgatt atagaaataa ttattctgcc
    45001 tttaaatcta tggggggagg agtagaatta gactgtatac atgaattgga ttatattata
    45061 aacctatttg gtttccctaa atcagtaaaa aaacagtttg gtaaatattc gaatcttgaa
    45121 attaacagta atgatttagc aacatatata ttgagatacg atgataaaat tatagaaatt
    45181 catttagatt attttggaag agttcctaca agaaaatttc aattgtttac tttagatgaa
    45241 tgtatcaatg ttgacttatt aaataataaa ataagttttg aagaaaacat tgttaaagaa
    45301 atgcctgaat ctgctaatga tatgtatgag aaggagttga aatatttctt tgaaaacgta
    45361 gtaacaggca aagaaaactg gaatgattta tatcatgcaa acgaagtatt gaaaatagct
    45421 gaggagaaat aaatggaaat tttatttact atttgtggta gagctgggtc taaggggttt
    45481 aaaaataaaa atttgaaata tttggcagga aagcccctag tccatttcgc tttagcaatg
    45541 atagatatct atcaacaaaa acatcctgaa cataattgca cgacttgttt gaatacggac
    45601 agtaatgagt tagtagaatt gacgactaag ttaaatccgt ctgtgaaaat tgtagaacgt
    45661 aaacctgaat tagctacaga tactgtaggg aaaattgaag tgatacgtga ttcattctta
    45721 caactagaaa gagaagataa tatatttgat ttaattatag atttagattt gacttcacct
    45781 ttacgtgaag tttctaatct tgaagaatta atagatgaac atctaaaaaa tttagataaa
    45841 gacgtctact tttcagttgt ttctgcaaga cgtaatccct attttaatat ggtaaaaaga
    45901 attgaaaatg aagttactat agttaataaa tcaagataca catctaggca acagacacct
    45961 gaagtttttg aaatgaatgc ctcaatgtat tcttttaaac ctgaatttct aaaaaaaagt
    46021 aattatattt ttgatgggca atgtgggata ataaaaatga gagattatct aattttagat
    46081 attgatagtg aagaagattt taaatggtta gaatttttgt ttcctaaatt tttagaagat
    46141 tcttcggagc ttcaagatat ctataattat ttatatcgga ggtaaaagga tgagctgtta
    46201 tcttatcacc ggtggggctg gcttcattgg ttctacctta gccaatcatt taggagaaga
    46261 acatgtggta atcgtcgtcg atgacttatc aatgggaaaa gtagaaaatc ttgatatgac
    46321 gaaaaatatc acttttattg agggagatgt cgctgagaaa tccttgatgg aagagatcat
    46381 gaaagctaat aaatttgatt atatctttca tttagcagca gttgctagtg tggcagattc
    46441 agttgcccga ccagtagaaa cacatcgtgt gaattttgaa agtgtattaa tgattttaga
    46501 attgatccgc aagtatcaac ctgatttaaa acggatcgtg tttagttctt cagcggctgt
    46561 ttatggggat gaaccgactt taccaaaaaa agaagagtcc gtgattcgcc cattgacgcc
    46621 ttatgcggtt gataaattcg ctgcagaaca atatgtgcta gattattgtc atttatatga
    46681 tgtacccggt agcgcagttc gttttttcaa tgtctatggt ccaaatcaaa atccgaattc
    46741 tccatacagt ggtgtgattt ctattctcgt agatcgctat aagaaacaaa tcgtaggaga
    46801 agcaacttca tttacattat ttggtgatgg aagccagtct agagactttg tttatataga
    46861 tgatgtaatc caagcattac ttttagttgc gaatgaggaa gctgcactgg ggaaacaatt
    46921 caatgttgga acgggtaaag caaccacact tttggaattg attcatgcaa tcaatgaaat
    46981 tctcggtgta gaattgacac ttgaatacaa agaagagcgt tcgggtgata tccatgactc
    47041 tctagcagat attactaaga tccagtcaat tggctatcaa ccgaaatatg gtattttaag
    47101 tggaatgact aattatttga aaactgaaat caattaaaac atactaagat aagattagag
    47161 ggtaaattca ggctctttgt caataaggac tgatgagttt tgtaaaatca aatctgggat
    47221 agaagggctc tttgtcaaat gatgttggta aagagaaatc ttagaataat ttggacagaa
    47281 cagaaagaag aaaacctctt tctgttctgt ttatttggtt aaaaatgttt tgccatgacg
    47341 aatcataatt cgtagtttga aattgtaaaa gttg
//
LOCUS       DASHHB010000018        45883 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.018, whole genome shotgun sequence.
ACCESSION   DASHHB010000018 DASHHB010000000
VERSION     DASHHB010000018.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 45883)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 45883)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..45883
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.018"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            9..731
                     /locus_tag="U2K71_001081"
     CDS             9..731
                     /locus_tag="U2K71_001081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM7315184.1"
                     /translation="MLLLTLVVGYFSIEFIHGFSSAKQSSTVKKVEPKSIPTTLNVAL
                     IGSDARSKDENGRSDSLLVAQYNQKTQQAKLISIMRDSYVDIPGYGMNKINAAYSYGG
                     VDLLNQTLKENFKFEAPYYASITFQDFIDCVNELFPGGVKIDAEKSLDLDGVYINKGE
                     QVMDGNTLLQYARFREDKEGDFGRIRRQQQVIKAISQQLKDVTSIFKLPKAVGKLLGS
                     IQTNLPESVLLDCGMDFLKDIC"
     gene            789..2828
                     /locus_tag="U2K71_001082"
     CDS             789..2828
                     /locus_tag="U2K71_001082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HEM7315185.1"
                     /translation="MKRSDKHGKNRTGAYIAGAVILIAAAGGGYFYYQHYQETQAVEA
                     GEKTVEQFVQALNKGDYNKAAEMTSKKAANKSALSEKEILDKYQNIYGAADVKGLQIS
                     NLKVDKKDDSTYSFSYKAKMNTSLGELKDLSYKGTLDRNDGQTTINWQPNLVFPEMEG
                     NDKVSLTTQEAARGNIIDRNGEPLATTGKLKQLGVVPSKLGDGGEKTANIKAIASSFD
                     LTEDAINQAISQSWVQPDYFVPLKIIDGATPELPAGATIQEVDGRYYPLGEAAAQLIG
                     YVGDITAEDIDKNPELSSNGKIGRSGLEMAFDKDLRGTTGGKLSITDADGVEKKVLIE
                     HEVQNGKDIKLTIDAKAQKTAFDSLGGKAGSTVATTPKTGDLLALASSPSYDPNKMTN
                     GISQEDYKAYEENPEHPFISRFATGYAPGSTFKIITAAIGLDNGTIDPNEVLTINGLK
                     WQKDSSWGSFQTTRVSSDVSQVDLKTALIYSDNIYAAQETLKMGEKKFRTGLDKFIFG
                     EDLDLPISMNPAQISNEDSFNSDILLADTGYGQGELLINPIQQAAMYSVFTNNGTLVY
                     PKLIADKETKDKKNVISETAVQTIVPDLREVVQDVNGTAHSLSALGIPLAAKTGTAEI
                     KEKQDVKGQENSFLFAFNLDNQGYMMVSMLENKEDDDSATKRASELLQYLNQNYQ"
     gene            complement(2937..4088)
                     /locus_tag="U2K71_001083"
     CDS             complement(2937..4088)
                     /locus_tag="U2K71_001083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HEM7315186.1"
                     /translation="MEFIGILCLILISTTIGSHISRRLGIPAVIGQLLVGVLLGQAGL
                     NWVKPDILVHDFSEIGVILLMFLAGLESDLSLLKKYFRPGMFVALLGIIFPVVFGWLT
                     GKAFQVSGNEAIFFGIILAATSVSISVEVLKELNVVNTKEGSTILGASVVDDILVVLV
                     LSFSLSFLTGEASSNASLPLVLIEQLIYFVFIFLLVKWIAPFLLSFAEKVYANSSIII
                     VSLVICLGMSYVADLVGLSSVIGAFFAGIAVSQTKVKEEVYHNVEALGYAVFIPVFFV
                     SVGLEVDFSDFSEQLLFISILTLVAILTKLLGGYFGAQIAGFSQHSSLMVGAGMISRG
                     EMALIILQIGQQSQLIEPQYYSPLVIVILLSTLISPLVLKYFTKKVYEV"
     gene            complement(4242..4772)
                     /locus_tag="U2K71_001084"
     CDS             complement(4242..4772)
                     /locus_tag="U2K71_001084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HEM7315187.1"
                     /translation="MFETIKQNIQKYALLRAAVYIISGIAIVLNPSAVFHFIGYLITA
                     YLVLLGLLNLFEAYRFKRKTGSWSIGLFSGLFFLILALIVYAFAPAIVSILPILLGLA
                     VIINSLFQLFFSMNTKAKSWSVYSSILLLIGGFVLLFNPFKSLMLLFQIFGFILIFMG
                     IQEIINFIRIRKMTIR"
     gene            complement(4871..5221)
                     /locus_tag="U2K71_001085"
     CDS             complement(4871..5221)
                     /locus_tag="U2K71_001085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2200 domain-containing protein"
                     /protein_id="HEM7315188.1"
                     /translation="MAQNRIFTTSFASVYPMYVAKAERKDRIKEEVDEIICWLTGYSQ
                     TELEYQLQKQVTFEQFFAEAPVPNPSRSLITGVVCGVRVEDIEDNLMKEIRYLDKMID
                     ELAKGKKMEKILRS"
     gene            complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="U2K71_001086"
     CDS             complement(5378..6907)
                     /gene="ahpF"
                     /locus_tag="U2K71_001086"
                     /EC_number="1.8.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkyl hydroperoxide reductase subunit F"
                     /protein_id="HEM7315189.1"
                     /translation="MLDKETKQNLEQYLALIESPIVFSVSLDNSENSQKLAEFTKEIA
                     EMSPKISWEKRDSMRTPSFSINPVGKESGIVFAGIPLGHEFSSFLLAMLQISGRAPKI
                     SESLSLKIKKITQTLHFDSYVSLTCHNCPDVVQALNILAVLNPNITHTMIEGGMFQKE
                     IDDKKIMAVPTVFLNGEEFSSGRMTIEQIVEKITGPLIEEELFEKEPYDVLVIGGGPA
                     ASSAAIYAARKGIRTGLVADSFGGQVVETLGIENMIGTSYTEGPKLMQQVESHVRSYP
                     IDIMMNQQAVSLNKEQHFINIGLANGISLKAKTAIIATGAHWRSINVPGEKEFKNKGI
                     AYCPHCDGPLFKGKEIAVIGGGNSGVEAAIDLAGLAKHVYVLEFLPELKADQILQDKL
                     VSLANVTVIKHAATKEISGTNQVESLSYIDRRTMEMHTLAVSGVFILVGLLPNTDWLD
                     GTIEMNPRNEIVTEKNGATNIPGVFAAGDCTDSPYKQIIVSMGSGATAALGAFDYLMR
                     T"
     gene            complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="U2K71_001087"
     CDS             complement(6917..7480)
                     /gene="ahpC"
                     /locus_tag="U2K71_001087"
                     /EC_number="1.11.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HEM7315190.1"
                     /translation="MSLIGKKIEEFNAHAYHQGEFLEVSEKDLMGNWSILCFYPADFT
                     FVCPTELEDLQEQYSTLKSLGVEVFSCSTDTHFTHKAWHDTSDAIGKIEYVMIGDPSH
                     QISRIFDVLDEEQGLAQRGTFIIDPDGIVQAMEINADGIGRDASALIDKIRAAQYVRT
                     HPGEVCPAKWKESGETLKPSFDLVGKI"
     gene            complement(7624..8286)
                     /locus_tag="U2K71_001088"
     CDS             complement(7624..8286)
                     /locus_tag="U2K71_001088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM7315191.1"
                     /translation="MKSSLQSQAYQAIRKQIIYADLEPGRKISEKALEEHLAIGRTPI
                     REALIQLRQQELVYTIPQSGTYVSKINLVSAENARFVREQLEEQIMIECCSKLDTQTR
                     IVLETILEEQEKAVKINDERAFFRADNLFHEACFEIAGRKEVWNWLDDHNTHLERFRW
                     LRVTIQGLPWETIMEQHYDLLKALVDKKPEEARFQTGLHLHMMLKEQETVTQRYPEYF
                     QQ"
     gene            8470..10116
                     /locus_tag="U2K71_001089"
     CDS             8470..10116
                     /locus_tag="U2K71_001089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HEM7315192.1"
                     /translation="MIDLNNWRNFIPELSQKEVEIPHFDRASLRENGQKNPVWIHFGG
                     GNLYRGFHAEIAQRLADLGELSTGVIVCETFDEEIIERAYQDFDDLILEVVMPSSGEF
                     VKRILAATADSLYCHPDCSEHFDKMIRHFENPSLQLVTFAITEKGYRLKDTNNKYIGP
                     IAVDIKNGPKKPSHTMSILAALLYKRFLSGAWPIALVSTDNFSQNGSQLRTSLLTIAK
                     EWQKEGYVQNTFIQYLSNEEIVAFPWTMIDRITPNPSLSVSKMLAKEGFSNMSIFQTA
                     KGNNIAPFVNTEDIHYLIMEDHFPNGRPKFEKAGVFLTDRATVDKADTMKVTTCLNPL
                     HTSLAIMGCLLGYRSIAEEMKDPDLSLLVSEIGYNEGLPVVESPGIIDPKIFLEEVIT
                     VRFSNPNIPDMPQRIATDTSQKVAIRYGETIKKYMEIEVKDPQTLVFIPLTLAAWLRY
                     LIGIDDQGKPFIPSPDPLLTDFQHKLSDIQLGTQDTSHIHEKMAPILSNKEIFGVDLY
                     QAELGRKIEEMFIKMVAGPNAVRKTLQDYLQEHGGNLSWK"
     gene            10107..11189
                     /gene="uxuA"
                     /locus_tag="U2K71_001090"
     CDS             10107..11189
                     /gene="uxuA"
                     /locus_tag="U2K71_001090"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEM7315193.1"
                     /translation="MEMTFRWYGSQEEKITLDEIRQIPAVKGIVGTLMDIPVGEVWPR
                     ERIKELKEEIESRGLTLKVIESVNVHDAIKIGLPSREQYIENYKQTIRNLAEYGIEVI
                     CYNFMPVFDWVKSDLDYRLEDGSSTLAFISDDIPDDPREIVARIEHSSNEFELPGWEP
                     ERLAHIKSLLEAYASVDEEKLRENFAYFLQSIIPTCEEVGVKMAVHPDDPPYSIFGLP
                     RIVKNREDLDWLCRIVDSPYNGITLCTGSIAEDPENDVYGILAEFCQRDRIPFAHVRN
                     IKFLANRDFYEAPHKSEYGSLDMYRILKTMYDNGFNEYIRPDHGRMIWNETGRPGYGL
                     YDRALGAVYLNGLWEAIEKGNKSKDN"
     gene            complement(11297..12850)
                     /locus_tag="U2K71_001091"
     CDS             complement(11297..12850)
                     /locus_tag="U2K71_001091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM7315194.1"
                     /translation="MQTQEAIKTFLLKKKSNTKLDIFLFLSEHRFFITTQYLADHFHM
                     SESNFLLYIKELEQDFERLNLTELHIDKQKPFLKLNFEGIDPAYCYYRLFGRYCNESV
                     SYQILTSLFSCQTNSIISLSQQTNYSASYLYTKMKKINAFLALYGLSISFSNNGRKSF
                     SGKEVQIQYAALDIYWNIFSSTATEFYDEDPQVVAFMMNTFFKKEVLDRLNSGMIDKL
                     YFLLKICKENFPATSVKDVQKEFTEYEYIDFFIDPSVDILRPNLPICREQRILVNILA
                     RLSITKIESEETSLEQYKLLKKANVPHVLYSREMVESFCTVFDLFIPEKEKILYSLAF
                     ARNKLYNGFLNFNQPNTPLPTFMLYQEHSVLKEVQTAIEQFYMDFREQNQHLLPVILE
                     KENVQWMVEDLVHLYDRYKKQPRIVIGVNYTRDFYISKDLIVKIEQIFSNESIKIQND
                     FMENCNIVISDCPLKQLPSHIKKIYILKGIITPEDWKKVIMRISEYIFELKEEALMDQ
                     SKKENQVTS"
     gene            12998..14286
                     /locus_tag="U2K71_001092"
                     /pseudo
     CDS             12998..14286
                     /locus_tag="U2K71_001092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318369.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
     gene            complement(14412..15491)
                     /locus_tag="U2K71_001093"
     CDS             complement(14412..15491)
                     /locus_tag="U2K71_001093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM7315195.1"
                     /translation="MNNVFIFCYLEVVDLTVYQRFLANEKIRRLTVLLFVVFVLFLVR
                     SMITTILLTFIFTYLMIHLVQIIQRFVRIPTGVLAIMIYTLIVYLIYLAFTKYIPMLI
                     HQTFDMIQSVINFYEHQPKDADPVLQYIHTYIERNDFFEQLQNGASIALGYLQDIGKM
                     AVAFAMSFILSFFFMIEKKKTIVFSRLFLKSEFSWFFQDIYYFADKFVNTFGLVLEAQ
                     FVVALLNTLLTTIALAVFGFHQLLSLAIMIFILSLIPVAGVIISCIPLSFIAYSQGGI
                     RDVVYILITILIVHLIESYVLNPKLMSSKTELPIFYTFVVLLVSERFFGVWGLIVGIP
                     IFTFFLDVLKVKKIPNHLPKETDIP"
     gene            complement(15594..16034)
                     /locus_tag="U2K71_001094"
     CDS             complement(15594..16034)
                     /locus_tag="U2K71_001094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM7315196.1"
                     /translation="MEDLYLVNQVCFPLYAASKEIIRHYTPLLKPLNLTYTQYLVMLV
                     LWEKKKTSVKELGNDLCLDSGTLTPLLKKLAEKNYINRQRETGDERIVNVTLTAQGAA
                     LQKQAEGIPQKIKSYLPLEQEELDTLQHLTRKMLDNFHQDQKKG"
     gene            complement(16091..16501)
                     /locus_tag="U2K71_001095"
     CDS             complement(16091..16501)
                     /locus_tag="U2K71_001095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="organic hydroperoxide resistance protein"
                     /protein_id="HEM7315197.1"
                     /translation="MKKMYSTKMINTGGRSGEVHNPDRTFELNIAAPGKRVENATNPE
                     QLFAAGFSACFNSALELIKQQKNISGASTVSAQVSLYSESETSFVLGVEIEGSIEGLS
                     LEETQELLEAAHKVCPYSKATAGNIEVTLKAVSE"
     gene            complement(16681..17115)
                     /locus_tag="U2K71_001096"
     CDS             complement(16681..17115)
                     /locus_tag="U2K71_001096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM7315198.1"
                     /translation="MHKGTEIDFVLNDSLKALELYQTIFDEIQLIEATDYPRGNNEVI
                     FTLYGTRFHMLDENTSLGLTAPTAEHPNTIWFNIIVSDIEDTHNKALRNGCVEIQEMT
                     EMMDFGVTNSMFRDPFGYLWMLHQVHREVIFEERKQLFEKYM"
     gene            complement(17215..18063)
                     /locus_tag="U2K71_001097"
     CDS             complement(17215..18063)
                     /locus_tag="U2K71_001097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM7315199.1"
                     /translation="MKETRQFIWTYIKIIFALFILGVAINMFLGPHHIAAGGVSGLGI
                     LLEAALGFDRAIVILVLNIVMLVLALLFLGKKPFFKVLFGSLVFPLIIAIVPETMITS
                     DRLLSVIFGSAIFALGVAILYKNNSSSGGTTIPPLIFKKYFHLNTSIGLFFTDAFVVS
                     LNLFIFGIEEFLYAILSIAITSIVMTYIETGINRKKSVMVMSEHYLEDIRLRLSAEIG
                     RGLTLLEAKGGYNRKSKEVLLIVVTDHEFSRMKPLIEEIDPSAFVIVSSVAEVMGRGF
                     TYHPIE"
     gene            18452..20218
                     /locus_tag="U2K71_001098"
     CDS             18452..20218
                     /locus_tag="U2K71_001098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-glycosidase"
                     /protein_id="HEM7315200.1"
                     /translation="MDTAAIYHRPESEYAFLYTNTRFRIRLRTRKNDIKKVYVLCGDP
                     YTITTEKWYQKQRPMKKCLSTNVHDYWEIEVTSETSRLQYAFHVVGEDNTDCFYGDQG
                     IFPYRENVITEPNFYFRIPYFHQIDRFTIPEWVKETVWYQIFPERFANGDKANDPEHT
                     LPWGSKDPDREDFFGGDLQGVMDHLDYLTDLGINGIYFCPIFKATSNHKYDTIDYYEV
                     DPAFGDKKLLKNLIDEAHKRGIRIMFDAVFNHMGVHSPQWQDVLKNGEKSIYKDWFHI
                     RFFPVDSYQMTDLPETAENIPYDTFAFTPFMPKLNTANPEVQKYLLDIATYWIKEFDI
                     DGWRLDVANEVDHHFWKKFREAVTEIKPDIYILGEIWHSSQAWLQGDEFHAVMNYAFT
                     DSIKDYFAKKKITASQMVSGMNHQQMLYRDQVNEGTFNLLDSHDTARILTLCQGNKEL
                     MKSVMAFMFLQKGSPCIYYGTEIGMTGEDDPDCRKCMIWEKEEQDLELFGFIKELVSL
                     RKQLSKIISEGSTQWLIVNDREDKLYFTRKLEGQIIYVYFNQSKEPWVVEQENEVILS
                     QNCQLLEDGKAEIRQYGFLIMC"
     gene            20320..21579
                     /locus_tag="U2K71_001099"
     CDS             20320..21579
                     /locus_tag="U2K71_001099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM7315201.1"
                     /translation="MKKRSVKQLGLGLISLGILGGLAACGNGNSSAEGEADEGKTLTV
                     SVDSGYKDYINEIKNTFEKENDVKIKLVEKDMFDQLESLALDGPAGKGPDVMMAAYDR
                     IGALGQQGHLAEVKLGNEAAYDETDKAQVTYDGKIYGEPAVIETLVLYYNKDLVDTAP
                     STFKDLENLSKDSRFAFESEAGKNTGFLAKWTDFYYSYGLIAGYGGYVFGDDGTNPSD
                     IGLNNAGAVEGISYATDWFKNVWPKGMQDIKSAGDFASQQFMSNKTAAIIDGPWQAQT
                     YKENNINYGVAKIPTLNNGQPYQPFGGGKGWVVSNYAKNKDLSQKWLDYVTNQENQEK
                     FFEMVNEIPANQQARETAKAKNDELTTAVIEQYETAQAMPNIPEMGEVWTGAENLMFD
                     AASGSKTPKESADEAVKTISEAIEQKY"
     gene            21726..23027
                     /locus_tag="U2K71_001100"
     CDS             21726..23027
                     /locus_tag="U2K71_001100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7315202.1"
                     /translation="MQQDKTSNINEKKITLCSLIPGMGQFVNGQAFKGIVFFAIFVLF
                     IGQMLLGGFHALVGLVTLGSIPMEDHSLFILIEGTLQFIITIIFLIFYGLNLLDAYRV
                     AKMRQEGKKINRTMKEVIHNIGDEGFPYLLTLPAYLLMIFTIIFPVLVTLFTAFTNYD
                     FNHIPPASLIDWVGAKNFFSIFFLSSYRNTFFAVFSWTVIWTLCATTLQITLGILTAV
                     VAHQKFIKFKRIFGIIFLLPWAVPAFITIMSFSNIFNDSVGAINTQVIPFINQLPFVD
                     ISAISWKTDPNWTKVAIILVQGWLGFPYIYVMVTGILQSISEDLYEAAKIDGANAWQR
                     FSNITLPAVFLVAAPTFITQYTGNFNNFSMIYLFNNGGPGSVGGGAGSTDILISWIYK
                     LTTGTSPQYSMASAITLIISVIVICVSLLVFKKTNAFNMGD"
     gene            23030..23881
                     /locus_tag="U2K71_001101"
     CDS             23030..23881
                     /locus_tag="U2K71_001101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7315203.1"
                     /translation="MKKFHSSARFQKRLSHFFTYFFLLILSVIIIYPLLITASTAFKS
                     GNIAAFSLDLSSDWTLGNFTRLFSETLYGTWYKNTLIISVLTMIVQVTVVTLAGYTYS
                     RYRFIGRKSSLMFFLVIQMVPTMAALTAFYVMAFLLEALDKYWFLTMIYIGGGIPMNT
                     WLMKGYFDTVPIDLDESAKLDGAGHFRIFFQIVLPLVKPMLAVQALWAFMGPFGDFML
                     AKFLLRTPENQTVAVGLQTFISDVTNQRVSLFAAGAILIALPICLLFFFLQKNFVTGL
                     TSGGTKG"
     gene            23894..24709
                     /locus_tag="U2K71_001102"
     CDS             23894..24709
                     /locus_tag="U2K71_001102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltodextrose utilization protein MalA"
                     /protein_id="HEM7315204.1"
                     /translation="MQTFPLNYFSALRTPTQVFAGRKLLSWPKFFLIFVFLVSLMVMP
                     VTLFYANQIQAIPLEQFLSVHSLIDEQGTQKFSELELSETGLQASQQTIAVTPEILVG
                     VSLSEKQQSDHGTFIDFEKEQWVIQQKDKSGIRRYTMNYSPSFQPDSVRTPEDFQRFL
                     EREFYASNRPTIILSYSLSLGLVLFVMTSLILFGASFFLWMTRKSQLSSIHTFKESAN
                     LMLNVMGIGSMAAAVVGFIHFDFILMLSVQTTITVLLLLWVFVKTRFKDKRVE"
     gene            24860..26026
                     /locus_tag="U2K71_001103"
     CDS             24860..26026
                     /locus_tag="U2K71_001103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM7315205.1"
                     /translation="MLEKTLYNQLFSRSFSLPVEVNYWDGTTKQYGDTDTPPQIKINI
                     HEEIPMKEITSNASLALGEAYMDHRIEIEGSIQALINDAYQQANSFLRGKDYLKWLPK
                     KEKHTKQKNKEDIHAHYDLGNDFYKLWLDPTLTYSCAYFNTPEDTLEQAQINKVHHIL
                     DKLFIKEGETLLDIGCGWGTLMFTATKEYNVKATGITLSEEQYDLITKRIKEEHLEDK
                     CRVLLMDYRELKGETFDHITSVGMFEHVGSENLEGYFKVVKDLLKPKGTALIHGISRQ
                     QGGATNAWINKYIFPGGYVPGVTELVDNITKNDLQLIDLESLRRDYQLTLEHWTKNFH
                     QVQDQVTKEMGDSFYRMWDLYLQACAASFESSNIDVIQYLLVQPGNNDIPMHRA"
     gene            26256..26903
                     /locus_tag="U2K71_001104"
     CDS             26256..26903
                     /locus_tag="U2K71_001104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEM7315206.1"
                     /translation="MKKTVIYTSLFAALFLAACQSNTNQGTSGNTSSSSEQRSSQVSD
                     SSTKASTSSSSQTKSSSEASTQSDIKVSAARAIELFQDKYPDTAITSLELDSDWGSYF
                     YKIEGVDDQKEYEARIDAQNEKMETENPETLDRDEQNGQKKKEDGLDVDKLITIEEAA
                     KIAVDKVGSGTATDWDLDKDLGTTYWEVKVKDGNQSTSVKINSQTGEVLSTEQDD"
     gene            26987..27931
                     /locus_tag="U2K71_001105"
     CDS             26987..27931
                     /locus_tag="U2K71_001105"
                     /EC_number="1.3.98.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate oxidase"
                     /protein_id="HEM7315207.1"
                     /translation="MSLETTFANHIFANPLMNASGVHCMTTQELDELAHSEAGAFITK
                     SCTINERKGNPEPRYFDVPLGSINSMGLPNLGFSYYLEYALAYEKVQENQNQPLFFSI
                     AGMSVQENLEMLEKIEKSGFNGITELNLSCPNVPGKPQLAYDFEATYETLKEVFSIFS
                     KPLGIKLPPYFDFAHFDQMADILNQFPLTYVNAINSVGNGLYIDTEQEAVVIKPKEGF
                     GGIGGEYIKPTALANVRAFYTRLKPEIQIIGTGGIRTGQDAFEHLLCGASMLQIGTEL
                     HKEGPEIFSRIIKELTQIMSEKGYTSIDEFKGKLRTIS"
     gene            28066..29517
                     /locus_tag="U2K71_001106"
     CDS             28066..29517
                     /locus_tag="U2K71_001106"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HEM7315208.1"
                     /translation="MNDATELAKELKEKEFSFQELVTDIQKKIKKQNHELNAFVALEP
                     FESNSHELLIPQSEKGPLAGIPFPLKMLGQEKKGWLATSGSRLFETHRASHTSNYVRQ
                     AEAIGLVPFGQTNAPEFGFKNITDPVIYGPARNPWNLECTPGGSSGGAAAAVSSGIVP
                     LAGASDGGGSIRIPASFCGLIGLKPSRGTMPVGPYAWRGWQGAAIDFGLTVSMRDTEA
                     LFYGMRSIHSGAPYQVSPVEWQTHPRKKRLKIAFCVDSPIHSQISEEAVLAVKKAVDF
                     LGKEGHDLTEIPYPLDGRKLITSYYQMNGAETAAMIRSIETGIQRAVRKEELEPISWT
                     LFQYGQKLSAATYIDSLHVWDEAAIVMENLHQEYDLFLSPTTAFTAPKISEDLQSDAI
                     RAQMEHISDCSEVEGLDVVSSMFEESLKITPYTQLANLTGQPAISLPTHLTKENMPLG
                     IQFMAARGREDLLFQIGYLFEANHQFHLPNPWK"
     gene            29538..30374
                     /locus_tag="U2K71_001107"
     CDS             29538..30374
                     /locus_tag="U2K71_001107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEM7315209.1"
                     /translation="MKKKRLFALITSAFFILSACGTAAANQEDNSWSNVEKKKELVVA
                     TSGTLFPASYYNDQNELVGYDVDLAKEVAKRLNVKISFKEYNVDGQITSVSKRESDFA
                     ANDFGITKEREKKFSLSETFKYSFDSMIVRKKDDSGIHSLEDLKGKKAAGEPNTSYMR
                     LAEKHGAKLVTYDNATNDQYLSDVANGRTDVILNDYYLQKMAVSALPDIPVKILENVY
                     FNENESGFLLKEGNDALKKKIDGVLKEMKEDGTTKKISEKYFQTDVSVKPKETMAESV
                     SE"
     gene            30374..31033
                     /locus_tag="U2K71_001108"
     CDS             30374..31033
                     /locus_tag="U2K71_001108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM7315210.1"
                     /translation="MYFPLIDFSLMWTSLPFVLQGLVYTLGIGFVSFLLGNLVGLLLT
                     VLGLLDWLPLNVFIRFYLSFFRGIPALVLLFLLYFGLPYQLSALTASVICFTITSSAF
                     IGEIYRGSLAGVSSGQWDAGLASGFSFFGVLKYIVLPQAFRISVPALSNVAMDLVKGT
                     SLAATITVPEIFQKAKIIGGRTFDYFSLYILVALIYWVICIGIEKVQQYLEKRSMHQS
                     I"
     gene            31159..31848
                     /locus_tag="U2K71_001109"
     CDS             31159..31848
                     /locus_tag="U2K71_001109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315211.1"
                     /translation="MKTPLTIEIESSLYQYCIEQGGLVVEEVTMPDDQGIVDTLSCLT
                     KFDGSREWRCYELKVTKADFRSTAKLSFVGHYNYFVLPSKLYEEVKTEIPTGIGVLVY
                     RPYTVEEEMPASGTFLIAKKAAKKELAVPEEALTNRFMASLFREVRKAKQMERGVKYF
                     PTDQLYKELKRRSENQDPFSNERYYERFLEEIASQAINELQEEVIALQHDYEYLRQQQ
                     QIRRLPTEPLE"
     gene            31854..32810
                     /locus_tag="U2K71_001110"
     CDS             31854..32810
                     /locus_tag="U2K71_001110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sce7725 family protein"
                     /protein_id="HEM7315212.1"
                     /translation="MYYPYLRGKQFDLLALKEALSRGLLSNKIQPVIEPVRDSATLKN
                     VIELFQKKHHPIAVIQNPQVGQFKLFDQHVHSWTVKENSSVVPAQILTPENLSEVLDS
                     PPAFLVFDGQHYPKDTEVWKQLAGVDSKFLIPDTSRFRIWLPENKIVIRDSFQTRKHV
                     ESYADKTDDFFSDDYLFFHADGYIGFSDFTIEGSRYFDKGFPSRALAIHLTYIDAYGN
                     IRVKHFVSDSNDSAKDQALKFLEAGDKMKKWIMRHHHQLLITSGMIELLALYQQQKFP
                     GLGVLKKWSLMHHLELISHLLDHPKDWLKYYSKVQELYEVIQ"
     gene            32854..33366
                     /locus_tag="U2K71_001111"
     CDS             32854..33366
                     /locus_tag="U2K71_001111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7315213.1"
                     /translation="MYIRQTRKEEVEEVVEIINAAADFIASQGSPQWQNGQKPTKETI
                     LNDIERKESYVLIVEDQIAGTAALVSSVDPVYTKIDGSWKESTGPYLSIHRVALAEST
                     RGKKLAKPFLEHLLTIAASKNIQDIRIDTYPANEPMEKTIYSAGFTYQGMIEFPFEHG
                     ERKAYQIIIN"
     gene            complement(33622..34170)
                     /locus_tag="U2K71_001112"
     CDS             complement(33622..34170)
                     /locus_tag="U2K71_001112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM7315214.1"
                     /translation="MNYCYVFIGPSGSGKTSLAEAIFQPEQKIITYTNRSPRPGEKDQ
                     EDYYFVSDNTFNQMIKGNEFAEWDQYAQHKYGSSKAEIKKKLTVGNCYAVLTANGFWH
                     LYEYFGTVVRPIFITISKETLQSRLKKRGDSSEQISARLAVYVEDLAQLKRLKAIPSL
                     LVVSNDGAFQEAADKIKKGLLI"
     gene            complement(34259..35440)
                     /locus_tag="U2K71_001113"
     CDS             complement(34259..35440)
                     /locus_tag="U2K71_001113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NupC/NupG family nucleoside CNT transporter"
                     /protein_id="HEM7315215.1"
                     /translation="MSYLLGIIGLFVTLGLAFLISSNKKAIKLKPLIVMFVLQLVLGF
                     VLLRTTFGTAVVSVLAKMFDHLLAFAGQGVDFVFAGVANKGSAPFFLSVLMPIVFISA
                     IIGILRYIKILPLFMKAVGLGLSKINGMGKLESYNGVASAILGQSEVFISIKKELPFL
                     SEKRLFTMSVSAMSTVSMSIVGSYMALIDSKYVITALVLNLFGGYILASIVNPYVLEE
                     KEDELIIEENKEQTFFQMLGEYILDGFHVAITVAAMLIGFVALIAMINAIFHGIFGIT
                     FQEILGYIFAPLAFISGIPWKEAVDAGSIMATKLVTNEFVAMTELANGNFHFTERTTA
                     IISVFLVSFANFSSIGIISGAMKGLNEEKGNLVAKHGLKILLTATLVSFLSAIVTGLL
                     V"
     gene            complement(35676..37184)
                     /locus_tag="U2K71_001114"
     CDS             complement(35676..37184)
                     /locus_tag="U2K71_001114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1846 domain-containing protein"
                     /protein_id="HEM7315216.1"
                     /translation="MKKIGFDSEKYIEEQSAYILERVNHYDKLYLEFGGKLVDDKHAK
                     RVLPGFEEDAKIKLLQKLKDQAEILICVYAGDIERNKIRGDYGITYDMDILRLIDELR
                     GYGLLINSVVITRYSGQPATKVFINKLERRNIKVYKHSEIEDYPVNVEKIVSEDGFGK
                     NEYIETTKPIVVVTAPGPGSGKLATSLNQLYHESQKGHAAGYSKFETFPVWNVPLKHP
                     LNIAYEAATVDLKDVNMIDSFHFDAYNKVAVNYNRDVETFPVIKRIIEKITGKESVYQ
                     SPTDMGVNRVGFGIVDDTVVQEASKQEIIRRYFQTECDFKKGLIDEEAVNRIKLIMEE
                     VGLRPEDRNVVVPAHDYADKIQAQSPTKDPSAVIAIELPDEVIITGRTSSLMDASAAV
                     ILNAVKHLAHIADDIPLLSPLVLETIQGLKSKTLHSSLDALSINEVLIALSISAVTNP
                     IAQVAYDKLAELEGAQAHSTVMVNKNDEQTLKKLGIDITCAPVYPSENLYYQ"
     gene            37343..37534
                     /locus_tag="U2K71_001115"
     CDS             37343..37534
                     /locus_tag="U2K71_001115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315217.1"
                     /translation="MKKRQKKKNAYKHYIRSIFTGYERMLEDPELEQLTFTYLNEETQ
                     LTRDEHQRIHFTTRDLPSK"
     gene            37737..38654
                     /locus_tag="U2K71_001116"
     CDS             37737..38654
                     /locus_tag="U2K71_001116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="viral A-type inclusion protein"
                     /protein_id="HEM7315218.1"
                     /translation="MKEPDTNANYEPDELEETNADQDTGQESSSFYDIKEKASMNLSQ
                     LLEDLQAFEKAVREEDIPEIYRLYNGRLNEELKRSSNENHEIDQLLMRKIHDRFLQEF
                     PFMEQVENISPTMSYYKIGTYYHDRPTVGIDASLPEIFVLPKIDEEWEKYSQPESTDY
                     DKKINELDAKVITAQTEIERLDEQIKEINRQMTDLNDNKGFLNRKKIEEEIQELEKKK
                     QVLSNEKLGWLPYIETPETIQQQKEALKQEARADQLRAAIVEKEQRQIRRYFGSKEGF
                     GQAIHEFLMNYLGNENPNNQSNEGGSEYE"
     gene            38647..39720
                     /locus_tag="U2K71_001117"
     CDS             38647..39720
                     /locus_tag="U2K71_001117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315219.1"
                     /translation="MNEHDEAEWTKNEEQTEDHEETNTSLEEQTSNPLDDTEEIPMLE
                     ETKIHEKEKVTPTNTNTAENTHAYEITENPGYLEGKEQDHQDIVSLIETAEAELADLR
                     MRKDLIESDFPQLLESYDRFILNEEPISTIGKTSLIEYFTYELLKPLNDIGLERSNSA
                     YDTWKTRHFSINYTLNGQNELVFSFVLPTIDQRYQVESMSLLNVSPETMEINVQDDRV
                     LSLIRHWSVDRVFSAGQITIFNHKLNQLLAHARTLGFTVNQTLLDNTKPLRLNLQSEF
                     ELTNEVLDDIFIVAMKNPSYDMEKINETTYQVRLDKGQSLTITTNEKDQTVLSISSGD
                     YPRSVIDFFINYEFLVPLMVRKS"
     gene            39868..40386
                     /locus_tag="U2K71_001118"
     CDS             39868..40386
                     /locus_tag="U2K71_001118"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315220.1"
                     /translation="MSTNKIAQLSLLSAACVAGRIAFQFIPNFQPMTAIFLFIVLYLS
                     LKDALIVMSLSIAISSFYFGVGPWVIGQWISYTVVLSLFSIVCLRKTVQRNLWLKGVC
                     FFLAGMVYGIGMTVFDTLLYRLPQPWIYYLQGVSFDLMHSIGNVVFFLVFLPVVKRFY
                     NHSGGKNEKNNL"
     gene            40367..40759
                     /locus_tag="U2K71_001119"
     CDS             40367..40759
                     /locus_tag="U2K71_001119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4430 domain-containing protein"
                     /protein_id="HEM7315221.1"
                     /translation="MKKIIYSLLAMSVSILILTSCSTTYTQSASNQKEEVKTSQKATI
                     TLQENGQNFSEKEVVFKKGDDLLSILKRNFEVKEDNGFITSLEGHSQDEKNQLYWMFT
                     VDGKSATTGAKEIKLNDGQSVIFNLSKL"
     gene            40835..41770
                     /locus_tag="U2K71_001120"
     CDS             40835..41770
                     /locus_tag="U2K71_001120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HEM7315222.1"
                     /translation="MENFKKSGMFSVIAALGANILVAISKFVGYAISGSAAMLNESIH
                     SIVDCSNQILLLFGDKQANKGQSELHQFGEGRAKYFFSTIVAMMLFFGGGALGVMEAF
                     EKLTHPSHEVGNPIIVIAILLFGLVIEGSSLRVAMKEIKELNTEKLSTMRFLRESRHS
                     EILIIFTEDLCAVIGLVLALAGTILTYATGIAAFDAISGLLIGFLLMGAAIFLAKEFY
                     SLIIGESVTASDLSKIKLAFERPEISRLIDIKTVHLSPTEILVAAKADIVGNKENEAY
                     SVINDIEKNMRQSLPDKKMYIYIETDKYDPNYSRK"
     gene            41903..42682
                     /locus_tag="U2K71_001121"
     CDS             41903..42682
                     /locus_tag="U2K71_001121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HEM7315223.1"
                     /translation="MNKKKSAPWLQALHAVMPLCISYIPVGLACGVLLQKVGFDPLLS
                     GLLSILVFSGGAQFLVASMLTTQASFATTLLMVFFLELRYTLLGASLAGFMKKEKRTF
                     LAVFSQSLNDENYAVNYLKFSTDPSWNKRKALYVNWFSMTAWAGSNMIGNFFGSVIRV
                     DADLVHFALTAMFIFMFIMQMKSALLIFTGLFSGVLGVVFMVLFQNTFGLIAATVIAS
                     FAGFMLEKAFKKEKAKKLRDRGKDVNEPLFNFPEQEANHHD"
     gene            42675..42992
                     /locus_tag="U2K71_001122"
     CDS             42675..42992
                     /locus_tag="U2K71_001122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HEM7315224.1"
                     /translation="MTKLYLLLLVACLFIVAYIPRLFPMLYFTHRKVPSWFSDWMKYV
                     PVALFAALAFKDVFITHEHLDIAWNIKIAAMILVAGVAYKTRSMALSVLTGLASVFLL
                     SML"
     gene            43236..44846
                     /locus_tag="U2K71_001123"
     CDS             43236..44846
                     /locus_tag="U2K71_001123"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HEM7315225.1"
                     /translation="MNKKLMQGTFWLTFANLLCKVLGVVYLIPWLSMMGNNQDGMLAT
                     TLYNVGYLPYGLFLMLGTVGFPNAIAKKVAVATKEGDNAACRLIFRSTINIMFVIGIA
                     SAALMYLFAPLLAGISPIANVNNGILAIRSLCPSLIAIPILSAMRGYFQGKNDLRPYG
                     TSLIIEQAVRVIVILAGTYYLRVLTDGTILEAVLISTVASFFGGLAAIIHMYIVGRRK
                     DFFELKDFLISSRYFERENRSASVSIIRETLPFIFVGSVITIVQMIDQVTMKPLLHFF
                     RPEIADQQLEFLFSRASVNPNKLTLILISMVGTVAISSLPILSTMKKKDRIQIEKTVG
                     DSFSIALLILLPSLTGMSLLAGPLYTLFFGYDPESVGYFQMALLASLFFSLFTILSTM
                     LQSLNHHRFAIRLTVEAIILKVIFQVIGLGLVGGYGMSLSNGLAFGIVFVRGYHFMCK
                     EYRIAPLAKISNFFLKTFRSTLIMLAVCFAVFFLLNQRLSMESKSYAMIYCVAVGSIG
                     GLVFLFSQFGKNGFRMLKNVKHHSRSKE"
     gene            45003..45851
                     /locus_tag="U2K71_001124"
     CDS             45003..45851
                     /locus_tag="U2K71_001124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM7315226.1"
                     /translation="MKLAVVTDSSAYLPERIKNHPDLFVIPIPLIMDGKIYNEGIDIE
                     ADEYYGLLKNSKDFPTTSQPVVGEVLGLYEELKEKGYDTILSIHLSSGISGFVNTLFA
                     MKDDISGVTVIPYDSKITSMPMGHMVEAALDLNDEGQSLDEILAHIDRIRDNTYAYLI
                     VDDLNNLVRGGRLTNGAALIGSLLKIKPILTFSEGKIVLFEKIRSSKKAFARAEEIIG
                     RRNNEIQPPVKLYVIHANNLEVAIAEKEKLQKKYPNAEIEIGHFGPVIGTHLGEKAIG
                     IAISAQ"
BASE COUNT        15235 a         8277 c         8822 g        13549 t
ORIGIN      
        1 tatctatttt gttactgctt acattagtag ttggctattt ttcgattgag tttatccatg
       61 gattttcgtc tgcaaaacag agttcaactg tgaaaaaggt agagccgaaa agtatcccta
      121 ccacactaaa tgtggctttg attggttcag atgctcgttc gaaagacgaa aacggtcgct
      181 cggattcact tctggttgca caatacaacc agaaaacaca acaagcaaaa ctgatctcta
      241 tcatgagaga ttcatatgtc gatattccag gttacggaat gaataagata aatgcagctt
      301 actcttatgg gggagttgat ttattaaatc aaacattaaa ggaaaatttc aaatttgaag
      361 ctccctatta tgcaagtatc acatttcaag attttatcga ttgcgttaat gaactgtttc
      421 ctggtggagt caagatcgat gcagaaaaat ctctagattt agatggcgta tatataaaca
      481 aaggagagca agtgatggat ggtaatactt tactccagta tgctcgattt cgggaagaca
      541 aagaaggaga cttcgggagg atcagaaggc agcagcaagt gatcaaagct atctctcagc
      601 agctgaaaga cgtaacttca atattcaagc tacccaaagc ggttgggaaa ttacttggta
      661 gtatccaaac gaaccttcca gaaagtgtgc ttctggattg tggtatggat ttcctcaaag
      721 acatatgtta gaataaacag gtataaatag tgaaataaag gaatgacaag caagagaagg
      781 aggaaaaaat gaaaagaagt gacaagcacg gcaaaaatcg aacaggcgct tatattgccg
      841 gcgcagtgat cttaatagca gctgcgggtg gcggttattt ttactaccag cactatcaag
      901 aaacccaagc agtagaagct ggagaaaaga cagttgagca atttgtccaa gctttgaaca
      961 agggagatta taacaaagct gcagaaatga catcaaaaaa ggcggcaaat aaaagtgcat
     1021 tatctgaaaa agagatctta gataaatacc agaatatata cggtgctgct gatgtcaaag
     1081 gacttcagat atcgaatcta aaagtagata aaaaagacga ttctacttac agtttttcat
     1141 ataaagcaaa gatgaatacc tcattaggtg aattgaaaga tctttcttat aaaggaacat
     1201 tagacagaaa tgatggtcaa accacgatca actggcaacc caacttggtt tttccagaaa
     1261 tggaaggaaa tgacaaagta agcctgacca cacaagaagc agcaagaggg aatattatag
     1321 atcgaaatgg tgaaccatta gcaacaacag gcaaactaaa acaattagga gtcgttccaa
     1381 gtaaacttgg agatggaggc gaaaaaacag ccaatatcaa agcgattgct tcctccttcg
     1441 acttaacaga agatgctatc aatcaggcga tttcacaaag ctgggtacaa cccgattact
     1501 ttgtcccatt gaaaatcatt gatggagcaa cgccagaact tccagctgga gctaccattc
     1561 aagaagtaga cggcagatat tatcctttgg gtgaagcagc tgctcagctg attggttacg
     1621 tgggagatat cacagcagaa gatattgata agaatccaga attaagcagt aatggaaaaa
     1681 tcggtcgctc aggtttggag atggcttttg ataaggatct tcgtgggaca acaggtggga
     1741 aattaagcat tacagatgca gacggtgtag agaaaaaagt tctgatcgaa catgaagttc
     1801 aaaatggcaa agatatcaaa ttgacaatcg atgcgaaggc acaaaaaaca gcttttgaca
     1861 gtctaggagg aaaagctgga tcaactgttg cgacaacgcc aaaaaccggt gatcttcttg
     1921 cgcttgctag ctctccaagc tatgatccaa acaaaatgac aaacgggatc tcacaagaag
     1981 attacaaagc ttatgaagaa aatcctgaac atccattcat cagccgattt gcgacaggtt
     2041 atgctcctgg atctacgttt aaaataatta cagcagcaat cggtctcgac aacggcacta
     2101 tcgatccaaa tgaagtgttg acgatcaacg ggcttaaatg gcaaaaagac agttcttggg
     2161 gatcttttca aacaacgcgt gtcagtagtg atgtatcaca agtagactta aaaactgctt
     2221 tgatctattc cgataatata tatgcggcac aagaaacgtt gaaaatgggt gagaaaaaat
     2281 ttcgtacagg cttagataaa ttcatttttg gtgaagacct tgatttgcca attagtatga
     2341 atccagcaca aatttctaat gaagatagct ttaactcaga tatcttgcta gctgatactg
     2401 gatatggaca gggcgaactt ctaattaatc ctatccagca agcagcaatg tattctgttt
     2461 ttaccaacaa tggcacactt gtctatccta aattgattgc agataaagag acaaaagata
     2521 agaagaatgt catcagcgaa acagcagtac aaacgatcgt gccagatctg agagaagttg
     2581 tgcaagatgt aaatggtaca gcacattctc tttctgcttt agggattccg ttggcagcga
     2641 aaactggtac agcggaaatc aaagaaaaac aggatgtaaa ggggcaagag aacagtttct
     2701 tgtttgcttt caatcttgat aaccaaggtt atatgatggt cagcatgttg gaaaataaag
     2761 aagatgatga ttcagcaacc aaacgagcat cggaactatt gcaatacctc aaccaaaatt
     2821 atcaataaaa aattgctttt taagtacagt aaaaggactg tggcgttatc gtcacagtcc
     2881 ttttttcaaa taaacggttt tcacgatgtc tctttcacaa cttctttttg atttactcac
     2941 acttcataaa ctttttttgt aaaatatttg agaacaagtg gagaaataag tgtacttaat
     3001 aaaatcacga taactaaagg tgaatagtac tgtggttcga tcaattgact ttgctgtcca
     3061 atttgtaaaa tgatcaaagc catttctcca cgcgaaatca taccagcacc gaccatcaat
     3121 gaactatgtt gagaaaaacc ggcaatctga gcaccaaaat aacctccaag caatttggtc
     3181 aaaatcgcta caagcgtcaa tatagaaata aagagtagtt gttcactaaa gtcagaaaaa
     3241 tcaacttcta atcctacact gacaaaaaat actggtataa atactgcata tcctaaagcc
     3301 tctacattgt ggtatacttc ttctttaacc tttgtctgac tgacagctat cccagcaaaa
     3361 aaagcaccaa tgacagagct aagcccaaca agatccgcta cgtaagacat tcctaaacaa
     3421 ataactagcg atacgatgat aatagaagaa tttgcgtaca ctttttctgc aaatgaaagt
     3481 aggaaaggcg caatccattt gactaataag aaaataaaaa caaaataaat tagctgttca
     3541 atcaaaacaa gcggtaaaga agcattgcta gaagcttccc ccgttagaaa agataggctg
     3601 aaacttagaa ctaaaacgac taaaatgtca tctaccacag aagcacccaa aattgttgat
     3661 ccttcttttg tattcactac gtttagttct tttaatactt ctactgaaat actgacagaa
     3721 gttgccgcaa gaataatccc aaagaaaatc gcttcatttc ccgaaacttg gaatgctttg
     3781 cctgtcaacc agccaaaaac aaccgggaag atgatcccaa gcaaagcaac gaacatacca
     3841 ggtcggaaat attttttcaa aagagataaa tcactttcca gaccagctaa aaacattaat
     3901 aagatcacac caatctccga aaagtcatgg actagaatat caggtttgac ccaattcaat
     3961 ccagcttggc ctagcaaaac accaacaagt aattgtccaa tcactgctgg gatacctaat
     4021 ctccgcgaga tatgtgagcc aatcgttgtt gaaatcaata tcaggcataa aataccaata
     4081 aattccattc tcatcattcc tttgtataaa atatagcccg aacttagcat aacatacatt
     4141 attgaaaaaa taggaaaata tactcacaaa aaaaccaaca atccaaaaga tttttttgga
     4201 ttgttggttt ttatacggtc tattttaata actaaaaatg attaacgtat agtcattttt
     4261 cttattctga tgaaattgat aatctcctgg atgcccatga aaatcaaaat aaagccgaat
     4321 atttggaata gaagcatcaa acttttgaat ggattgaaca ataagacgaa tccgccaatc
     4381 aacaacagaa tactactata aacactccaa cttttagctt ttgtattcat gctgaagaac
     4441 aactggaaca agctgttgat gataacagct aatccaagaa gaatcggcag gatagaaacg
     4501 attgcaggtg caaaagcata gacgatcaga gctaagatca ggaaaaaaag accactgaac
     4561 agaccgatac tccaagaacc tgttttcctt ttaaaacggt aagcctcaaa aagattcaac
     4621 aacccaagca aaacaagata ggcggtgatc agataaccga taaaatgaaa aacagcagaa
     4681 gggtttagca caatcgcaat cccagaaata atataaacag cagctcttaa taaggcgtat
     4741 ttttgaatat tttgtttaat cgtctcaaac atataagaaa acctcctttg acttatttta
     4801 acgtgaacta gtcattttac caaattaaat gttacaaaat gagcgaagga gaagaatatc
     4861 tctggaaaaa tcaacttctg aggatctttt ccattttttt gccttttgct agctcatcga
     4921 tcattttatc taaataacga atttctttca tcaagttatc ctcgatgtct tctactcgta
     4981 caccacaaac aacacctgta atcaaggaac gtgaaggatt ggggacaggg gcttccgcaa
     5041 aaaattgttc gaaagtcact tgtttttgta gttgatattc tagttctgtt tgggaataac
     5101 ccgtcagcca acaaatgatt tcatctactt cctcttttat acgatctttt ctctccgctt
     5161 ttgctacata cataggataa acgcttgcaa aacttgtcgt aaaaatacga ttttgcgcca
     5221 taatcagtca cctttctttt tttcaaatta tttactactt atagtatata agaaaaagag
     5281 aagaaaaagc gattattcaa aaaaacaaaa aggattgtga caaaattctt gtcaccatcc
     5341 ttttttccgc tctctgactt tttacaatct tcatagctca agtgcgcatc agataatcga
     5401 atgctcctaa agctgcagta gctcctgaac ccattgaaac aatgatttgt ttgtatggac
     5461 tatctgtaca atcaccagct gcgaaaacgc cggggatatt cgttgcgcca tttttctcgg
     5521 tcactatttc atttcttgga ttcatttcga tcgttccatc tagccaatcc gtattaggga
     5581 gtaagccgac tagaatgaat actcctgaaa cagctagtgt atgcatctcc atcgtacggc
     5641 gatcgatata ggagagagat tctacctgat tcgtaccgct gatttctttc gtcgctgcat
     5701 gtttgataac agtgacattc gcaagagaga ctagtttatc ctgtaagatt tgatctgctt
     5761 ttaattctgg taagaattct agaacatata catgttttgc taacccagcc aaatcgattg
     5821 ctgcttcaac accagaatta ccaccaccga taacagcaat ctcttttcct ttaaataaag
     5881 ggccgtcaca gtgtgggcaa taagcaattc ctttattttt aaactctttt tcgcctggta
     5941 cattgataga acgccagtgc gcacctgtcg cgatgatggc ggtttttgct ttaagtgata
     6001 taccgttagc taagccaata ttaataaaat gttgctcttt atttaaggaa acggcctgtt
     6061 gattcatcat aatgtcgata gggtaactac gcacatgact ttctacttgc tgcatcaatt
     6121 ttggaccttc tgtgtaagag gtaccaatca tattttcaat ccctagtgtt tcaacgactt
     6181 gtccgccgaa agaatcagcg acaagtcctg tacggattcc tttacgtgct gcatagatag
     6241 ctgcactact agctgctggt ccaccgccaa ttactaatac atcataaggt tctttttcaa
     6301 acaactcttc ttcaatcaaa gggccagtga ttttttcaac aatttgttcg atcgtcatgc
     6361 gtccgctact aaattcttct ccatttaaaa acacagttgg cactgccatg atttttttat
     6421 catcgatttc tttttggaac atcccacctt caatcatcgt atgagtaata tttggattta
     6481 atacagctaa tatattcaat gcctgtacaa cgtcggggca attatgacaa gtcaagctaa
     6541 cataactgtc aaaatgcaat gtttgtgtta tttttttgat tttaagactc aaagattcac
     6601 tgattttcgg tgcccgtcca ctaatttgta acatagccag taaaaaagag gaaaattcat
     6661 gaccgagggg gataccagca aaaacgattc cactttcttt gccaactgga ttgatagaaa
     6721 aactaggtgt gcgcatcgaa tctctttttt cccaagagat tttaggagac atttctgcta
     6781 tttccttcgt aaattcagct agtttttgac tattttcact attatccaaa ctaacagaaa
     6841 aaacaatagg agattcaatc agtgctaaat attgttcgag attttgttta gtttctttgt
     6901 ctaacatatc caccacctaa atctttccaa caagatcaaa acttggtttc aatgtttcgc
     6961 cactttcttt ccattttgcc ggacaaactt cacctgggtg ggtacgtaca tattgagcag
     7021 cgcgaatctt atcaatcagt gcactagcgt cacgaccgat tccatctgca ttgatttcca
     7081 tagcttgaac gattccgtct ggatcaataa taaatgttcc tcgctgagct aatccttgct
     7141 cttcatccaa aacatcaaaa atacgagaaa tctgatgaga cggatcaccg atcatcacgt
     7201 attcgatttt accgatagca tctgatgtat catgccatgc tttgtgagta aagtgggtat
     7261 cggtagagca agaaaagact tcaacaccta aagatttcaa tgtactgtat tgttcttgta
     7321 agtcttctaa ttcagtagga caaacaaaag taaaatcagc tggatagaag caaagaatac
     7381 tccagttccc cataagatct ttttcactga cttctaaaaa ttctccttga tgataagcat
     7441 gtgcattaaa ttcttcgatt tttttcccaa tcaatgacat agtaaaacct ccaataataa
     7501 ttatttattt ataattattg taatttagtt ggagttgcaa agtcaatgga tattttgtta
     7561 tttaataaag attactcaaa aacgaaggtt aattataaaa atagaagagg tcgcaagaaa
     7621 atgctactgc tggaaatact cgggataacg ttgagtaaca gtttcctgtt ctttcagcat
     7681 catgtgtaag tgcaatcccg tttgaaatcg agcttcttct ggttttttat caactaaagc
     7741 cttgagtaaa tcataatgtt gttccatgat cgtttcccat ggaagcccct gaatggtcac
     7801 tcgtaaccag cggaaacgtt ctaaatgggt attatggtca tctaaccagt tccaaacttc
     7861 ttttcttcct gcaatttcga aacaagcttc atgaaataaa ttatctgcac ggaaaaaagc
     7921 gcgttcgtca tttattttga ctgctttttc ttgttcttct aaaatcgttt ctagcactat
     7981 tcgtgtttgt gtgtccaatt tggaacaaca ttcaatcatg atttgctctt ccaactgttc
     8041 tcggacaaag cgagcatttt ctgcagaaac aagattaatt ttggaaacat acgtaccact
     8101 ttgaggtatc gtatacacga gttcttgctg acgtagttga atcaatgctt cgcgaatggg
     8161 agtccggccg atagcaagat gttcctccag tgccttttca gatattttcc ttccaggttc
     8221 taagtcagca tagataattt gtttacgaat cgcttgataa gcttgtgatt gcaagctgct
     8281 tttcatcttt attcctccaa aatcatttct atgaacgtag tataacagaa gaggtctgaa
     8341 aaacaatctg gtatgtcaca ttaactagta aatttgtata aatagtaaaa ttttggttga
     8401 catagcgatt tttattgtat aaaattcact tgaatacaaa aactaatatg ttagtttagg
     8461 aggggaaaaa tgattgattt aaacaactgg aggaacttta tccctgaatt atctcaaaaa
     8521 gaagtagaaa tacctcattt tgaccgtgca tcattaagag aaaatggaca aaaaaatcct
     8581 gtctggatcc atttcggtgg cggaaatctt tatcgaggat ttcatgcaga aatcgctcaa
     8641 cgtttagcgg atctaggcga attgtccact ggtgtcattg tttgtgaaac attcgatgaa
     8701 gaaataattg aaagggctta ccaagatttt gatgatttga ttcttgaagt ggtcatgcca
     8761 agcagtggag agttcgtcaa acgtatatta gctgcgacag ctgacagtct gtattgtcat
     8821 ccagattgtt ctgaacactt tgataagatg attcgacact ttgaaaatcc tagtttacaa
     8881 ttagttactt ttgctattac tgaaaaaggt tatcgattga aagatacaaa taataaatat
     8941 ataggaccga ttgcagtcga tataaaaaat ggtccaaaga agccatcaca tacaatgagt
     9001 attcttgctg cgctacttta taaacgtttt ttatctggag cttggccgat tgcacttgtc
     9061 tctacagata atttttcaca aaacggtagt caattacgta cttctttgct aacgatcgca
     9121 aaagaatggc aaaaggaagg ttatgtacaa aatacattta tccaatactt atcgaatgaa
     9181 gaaattgttg cgtttccatg gacgatgatt gatcgtatca ccccgaatcc ttccttgtca
     9241 gttagcaaaa tgttggcaaa agagggcttt tcaaatatgt cgattttcca aaccgcaaaa
     9301 ggaaataata ttgctccatt tgtcaataca gaggacatcc attatttaat catggaagat
     9361 cattttccca atggacgtcc gaaattcgaa aaagctgggg tattcttgac tgatcgtgca
     9421 acggtagata aagcagatac gatgaaggtg acgacttgcc ttaatccttt gcatacgtct
     9481 ttagctatta tgggttgttt actggggtat aggagcatag ctgaggagat gaaagatcca
     9541 gatttatcgc tgctagtttc tgaaatcggg tacaatgaag gcctcccagt tgtggagtct
     9601 ccaggtatca ttgatccaaa gatctttctg gaagaagtaa ttactgtgcg cttttctaat
     9661 ccaaatattc ctgatatgcc tcagcgaatc gccacggata cctctcaaaa ggtggcgatt
     9721 cgttatggtg agacgattaa aaaatatatg gaaatagaag ttaaagaccc gcagacactt
     9781 gtattcatac cattgactct cgctgcttgg cttcgatacc ttattggcat tgatgatcaa
     9841 ggaaaaccat tcatcccgag tccagaccca ctattgacag actttcagca taagctttcc
     9901 gatattcaac tgggtacgca agatacatca catattcatg agaaaatggc tcctattttg
     9961 tcaaataaag aaatttttgg tgtagatctt taccaagcag agcttggcag aaaaatagaa
    10021 gaaatgttca tcaagatggt agcaggaccg aatgccgtac gaaaaacatt acaggattac
    10081 ttacaagaac atggaggaaa tttatcatgg aaatgacttt tagatggtac ggatcacagg
    10141 aagaaaagat taccttagat gaaatcagac agattcctgc ggtcaaaggg attgtaggca
    10201 cattgatgga tataccagta ggagaggtat ggcccaggga gaggatcaaa gaactaaaag
    10261 aagaaattga gtctcgtggg ttaacactaa aagtaattga aagcgtcaat gtgcatgatg
    10321 ccattaaaat tggattacct tcccgtgagc agtatatcga aaactataag cagacaatcc
    10381 gaaacttggc agaatatgga atagaagtca tctgctataa ttttatgcct gtatttgact
    10441 gggtgaaatc tgaccttgac taccgcttag aagacggctc atccacgtta gcttttatat
    10501 ctgatgatat tccagatgat ccaagagaaa tcgtcgcgcg catcgaacat tccagcaatg
    10561 aatttgaatt gccgggatgg gaacctgagc gcttagctca tatcaaatcc ttgcttgaag
    10621 cctatgcatc agtagacgaa gaaaaacttc gagaaaattt tgcctatttt ctccaatcaa
    10681 tcatcccaac atgtgaagag gtaggggtca aaatggctgt acatccagat gatccgccat
    10741 actcgatttt tggattacct cgaatcgtaa agaatcgaga agatttagat tggttgtgcc
    10801 gcatagtaga cagcccttat aacggaatca ctctttgtac agggagtatt gcagaagatc
    10861 cagaaaatga tgtatatggg attttagcag aattttgtca aagagatcga atcccatttg
    10921 cacatgttcg aaatattaaa tttttagcta atcgagattt ctatgaggca ccgcataaat
    10981 ccgaatacgg ttcattagac atgtaccgca tactaaaaac aatgtatgac aacggattta
    11041 acgaatacat ccgtcctgat catggaagaa tgatctggaa tgagacagga agaccaggat
    11101 acggcttata tgatcgagca ttaggtgctg tttatctaaa tggattatgg gaagcaatcg
    11161 aaaaaggaaa caaaagtaaa gataattagt gaaaaaaact gtaattttat tatgtaaata
    11221 agaaggatgt tcaaattctt gatacataaa tccggaggtt gttaataagc attcggattt
    11281 atgtatctta tttggtctag gaagtaactt gattttcttt tttcgattga tccatgagtg
    11341 cttcttcttt taattcaaag atatattctg agatcctcat aatcactttt ttccagtctt
    11401 ctggtgtgat gattcctttt aaaatataga tttttttgat atgagaaggc aattgtttca
    11461 aagggcagtc gctgataacg atgttgcagt tctccataaa atcattttgt attttgatcg
    11521 attcatttga aaaaatttgt tctatcttaa cgatcagatc tttactgata taaaaatcac
    11581 gcgtatagtt cacaccaatc acaattcgcg gctgtttttt gtagcgatca tacaaatgta
    11641 ctaggtcttc aaccatccat tgcacatttt ccttttctaa aataactggt aacaagtgtt
    11701 gattttgttc tctgaaatcc atataaaatt gttcaatggc ggtttggact tctttcaaca
    11761 cgctatgttc ttgataaagc atgaaagtag gaaggggcgt atttggttga ttgaagttaa
    11821 gaaatccatt ataaagttta tttcgtgcga atgccaatga gtaaagaatc ttttcttttt
    11881 caggtataaa caaatcaaat acagtacaaa aggactccac catttctctt gaataaagaa
    11941 catgaggaac gtttgctttt tttaaaagtt tgtattgctc caagcttgtt tcttccgatt
    12001 cgattttcgt gatagaaagt cgagctaaaa tattcactaa gatgcgttgt tcgcgacaaa
    12061 ttggcagatt aggtcgcaaa atatctacag agggatcaat aaaaaagtca atatattcat
    12121 actctgtgaa ttctttttgt acgtctttca cagaagttgc aggaaaattc tctttacata
    12181 tttttaatag aaaatataat ttatctatca taccgctatt tagtcggtca agtacttctt
    12241 ttttaaaaaa agtattcatc ataaaagcca ctacttgcgg atcttcatca taaaattctg
    12301 tagcagtact tgaaaagatg ttccaataaa tatctaatgc tgcatattga atttgtacct
    12361 cttttccaga aaaagacttt ctgccattat tagaaaagga gatagacaaa ccgtacaatg
    12421 ctaaaaatgc attaatcttt ttcattttcg tatacaaata agaggcacta tagtttgttt
    12481 gctgacttaa actgataatc gaatttgtct gacagctgaa caaagaagtc aagatctgat
    12541 aactgactga ttcgttgcag tatcttccaa atagacgata atagcagtaa gccggatcaa
    12601 ttccttcaaa attcaacttt aaaaaaggtt tttgcttatc aatatgcagt tctgttagat
    12661 tgagacgctc aaagtcttgt tctaattctt tgatgtatag taaaaaattt gattcagaca
    12721 tatggaaatg atctgctaaa tattgtgtag taataaagaa acgatgttca gataggaaaa
    12781 gaaaaatatc taattttgta ttgctttttt tcttcaagag aaacgtttta atcgcttctt
    12841 gagtttgcat gaaagaggct cctttttact taaattgtta aaacttgaaa atattactgt
    12901 ttttttcata aatttagaca aaaaaacagt aaacatatag gtagaatctc tactatctta
    12961 tttcttgcat acaaaaaaat caaggagtga gagagttatg agatattcaa ccatcttaat
    13021 atcaacattt gcagccttaa gcttatcgct tactataaat cagacagtcc acacagagga
    13081 gatagaaaca gccaaatgga cagaaaccaa gtgggccaac gtcaatcgaa ttacctttca
    13141 ggatgaattt tcatccttca attcaggagt cgcaaaatta agaaaaggac aaacagcgac
    13201 catcaaagca aaaattgact attcaggaga cttagcacct atactacaat cagctgttac
    13261 atttgagaat gttgatcccg ctttgtcgat tggctatgac gagaacactt tgactatcca
    13321 acacaagcaa gcgatattta ccttcaccgt tacattaaat caggacttgt taaaaccagc
    13381 gctttttact gtcaaggtat cagatggact accgaacgat catcatcatt taacgccgta
    13441 tagtcagcgg cagacaattg agcaagattc agctatcgac tcaggaggag atcttgttga
    13501 agaaccaaca gataaaccag aaaatgaaaa taagccagaa gtaccgccta cagaaaatcc
    13561 agacggagaa cagaaaccgg aaatagaacc tggagaagaa cccgatacag aaactcaacc
    13621 agagccagac aatgaatcaa aacctgaaat aacccctgga gaaaaaccag atgtagaccc
    13681 agaagaaaaa ccagatgtga ctccagaacc agatacggat tctggaaacc aaacagtacc
    13741 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagcccc
    13801 agaagaaaaa ccagatgtaa ctccagaacc agatacggat tctggaaacc aaacagtacc
    13861 ggaaacaaat ccagacacag ataatgaaac tgaaaatcca gaaaaaccag aggtagatcc
    13921 agaagaaaaa ccagatgtga ctccagaacc agatacggac gcaagagacc aaggaatacc
    13981 agaaaaaata aacaaaaaaa caattcaaga ggatggaaaa aaagagagta aaaaatcaaa
    14041 tctagcaatt ctaaaaataa atgaagagca gctgaataaa aaatcacgaa tatttgatag
    14101 cgcccaatca gctgaaacct taaaaagtag caaggataca acctttgctg tcctgaaacg
    14161 aaaaataaac agctaccaaa atccggagag tcacaaaaca aagttatact gtggagcgga
    14221 atcattctat taagtatagc aacaatgctt tctgcaaaaa gatttaaaca aaacagatct
    14281 ctctaacatt tgaataatgt ctgtgaacgc atttttcaaa taaaaagaaa tatccagaaa
    14341 atgaccccaa aataccagat tgaaatctga tatttggggt cgttcatact ttatctttct
    14401 tcatatttat tttaagggat atctgtttct tttggaagat ggttcgggat ctttttcact
    14461 ttcaatacat ctaaaaagaa ggtaaagatc ggaatgccga caatcaagcc ccaaacgcca
    14521 aagaaccgtt cgctaacaag taagaccaca aatgtataaa aaatcggcag ttctgttttg
    14581 ctggacatca acttcggatt cagcacataa gactcaatta gatggacaat caaaatcgta
    14641 atcaggatat aaacaacatc tcggatgcct ccttgggaat aagctatgaa agatagagga
    14701 atacaagaaa tgataacacc tgctactggg atcagactta aaatgaaaat catgatcgct
    14761 aaactcagca gctgatgaaa gccaaataca gccaaggcga ttgtagtgag caacgtattt
    14821 aaaagagcga caacaaattg agcttctaat accaaaccaa atgtattgac aaacttatcg
    14881 gcaaaatagt aaatatcttg aaagaaccaa gaaaattcac tttttaaaaa caatcttgag
    14941 aatacaattg tttttttctt ttcaatcata aaaaagaaac ttaagatgaa tgacatagca
    15001 aacgccactg ccatttttcc tatatcctgt aaatagccaa gcgcaatcga agcgccattt
    15061 tgaagctgtt caaaaaaatc gtttcgctca atataagtat ggatatattg taaaacagga
    15121 tctgcatctt ttggctgatg ctcgtaaaaa ttaataacgg actggatcat gtcaaatgtt
    15181 tgatgaataa gcatcgggat atactttgtg aatgccagat agataaggta aacgatcagt
    15241 gtataaatca tgatagctaa aacacctgta gggattcgga caaatcgctg aatgatttga
    15301 acaagatgaa tcatcaaata agtaaagata aatgtgagca aaatggttgt aatcatactt
    15361 ctaactaaga aaagaacaaa aacgacaaat aataaaacag tgagacggcg tattttttca
    15421 ttcgccaaaa aacgctgata aacggtcaaa tcgacaacct ccaaataaca aaaaataaac
    15481 acgttattca ttatatgatg gaatatgaaa ataagaaaga gcaaaaagaa aaaccgctaa
    15541 atgacgttat cttatgagtt ggttttcctc ataaaatcgt tcatctagca gtttcagccc
    15601 tttttctggt cttgatgaaa attatctagc atttttcggg tcagatgttg cagtgtatcc
    15661 aattcttcct gttcaagtgg aagataagac ttgatctttt gtggaatacc ttctgcttgt
    15721 ttttgcaacg cagcaccttg agcagtcagc gtaacattta cgattctttc atcacccgtt
    15781 tcacgctgac gattaatata atttttttct gctaactttt ttagtaaggg agtcagcgta
    15841 ccagaatcca gacacaaatc atttcctagc tctttcactg aagtcttttt cttttcccat
    15901 aaaactaaca tcacaagata ctgggtatag gtaagattca gcggcttgag gaggggagtg
    15961 tagtgtctaa tgatttcttt agaggcggca tataaaggaa aacatacttg attaactaaa
    16021 tatagatctt ccatttttcc acctccgctg ttttactata gtgtatcata gcggatttac
    16081 acaaaaaagt ctattcacta actgctttca gagtaacttc tatatttcca gctgtagctt
    16141 ttgaatacgg acacacttta tgcgctgcct ctaataattc ttgcgtttct tccagagaaa
    16201 gaccttcgat agatccttca atctctacac ctaagacaaa gctcgtttca ctttctgaat
    16261 aaagggaaac ttgtgcactg acagtagaag ccccagagat atttttttgc tgtttgatca
    16321 attctaatgc actgttaaaa catgcactga aaccagctgc aaacaattgt tcagggttgg
    16381 ttgcattttc gacgcgttta ccaggagctg caatattcaa ttcgaatgta cgatcgggat
    16441 tatgcacttc tccagagcgt ccgcctgtgt tgatcatttt cgtagaatac atttttttca
    16501 taaaattacc acctttaatt atattttata caattgaatt ttacacaata taattgtgga
    16561 aagcaaagaa atggattggc ataagataaa taagagaaag cacatgaaac agacgcgctt
    16621 tctcgaatag caaacatata caaaaaatat cagagacaaa aaataatctc atcataaaca
    16681 ttacatatat ttctcaaaaa gttgttttct ttcttcaaaa attacttcac gatggacctg
    16741 atgaagcatc cataaataac caaaaggatc tctgaacatt gagttagtca caccaaagtc
    16801 catcatttct gtcatttctt gaatctctac acagccattt cgtaatgctt tattatgtgt
    16861 gtcctcgata tcagagacga tgatattgaa ccagatcgta tttggatgtt cagcagtagg
    16921 agcagtcaaa ccaagagacg tgttttcatc cagcatatgg aatctcgtac catacaaggt
    16981 aaaaatgact tcattattgc ctctagggta atctgttgcc tcaatcagtt gtatttcatc
    17041 aaagattgtt tgatacagtt ctagtgcttt caaactatca tttaatacaa aatcgatttc
    17101 cgttccttta tgcataaaga tctcctctcc ttttcagtcg aacagttact agattctttt
    17161 attataccag ctaaagaaaa cgcattcaaa aaaatagcgg atttacttac caactcattc
    17221 gattggatga taagtaaatc cgcgtcccat tacttctgct acgctgctga caatgacaaa
    17281 ggcacttgga tcgatttctt ctatcaatgg tttcattcgg gaaaattcat gatcagtcac
    17341 aacgatcaat aaaacctctt ttgactttcg attatacccg ccttttgcct ctagcagggt
    17401 aaggccacgt ccaatctcag cacttaagcg aaggcggatg tcttccaaat aatgttcact
    17461 catgaccata acagatttct ttcgattgat acctgtttcg atataagtca tgacaattga
    17521 agtgattgca atagataaaa tagcataaag aaactcttct attccaaaaa taaacagatt
    17581 aagagaaaca acgaatgcgt ctgtaaaaaa taagccaatc gaagtattca agtggaaata
    17641 ttttttaaaa atgaggggag ggatcgtcgt accgccgcta gaagagttgt ttttatataa
    17701 aatcgcaact cctaaagcaa aaatcgcact tccaaaaatg acagacaaca accgatctga
    17761 agtaatcatt gtttctggaa ctattgcaat gattaaagga aaaaccaagc ttccgaaaag
    17821 tactttaaaa aagggttttt tacctaaaaa tagaagagct agtaccaaca taacgatatt
    17881 caacactaaa ataactatag ctcggtcaaa gcctaaagca gcttccagta atattcctag
    17941 accgctgaca cctccagcag caatatgatg aggccctaaa aacatattga tagcgactcc
    18001 taatatgaag agggcaaaaa taatcttaat gtatgtccat ataaattgtc tcgtttcttt
    18061 catttattca cttccttaat tcattaataa tactcttatc ttattataac ttacttttca
    18121 ccttaagtta actttaatta ataaaaaaat taggctagtc taaattatta tgtttcattc
    18181 ttctagaata tacttctacg ccaaaaactg ttaaaaaact tttactttag aaaattggag
    18241 aaattgatcg cttaagataa attcaggaca ctaaatagta caggaagtag actaaaactt
    18301 ttaaaagttt taagtctatc tatttggcaa gtttaaaaaa taaaaattcg gtatcaaaaa
    18361 actgttaccg ataacatttt tgaaagcgtt gtcttttgct tctctatcac ttatagtgat
    18421 gacgtcatat taaaacacaa ggaggaaatc aatggataca gctgcaattt atcatcgacc
    18481 ggaaagtgaa tatgcatttc tatatacgaa tacaagattt cgtattcggc ttcgtactag
    18541 gaaaaatgat atcaaaaaag tatatgtgct atgtggagat ccatatacca taacaactga
    18601 aaaatggtat caaaaacaac gaccaatgaa aaaatgtcta agtaccaatg ttcatgatta
    18661 ttgggaaatt gaagtgacga gcgaaacaag tcgcttacag tatgcgttcc atgttgttgg
    18721 ggaagataac acggactgtt tttacggtga tcaaggaatt tttccttatc gagaaaatgt
    18781 gattactgaa ccaaattttt attttcgaat accttatttt catcaaatcg atcgattcac
    18841 tattccagaa tgggtaaaag aaacagtatg gtatcaaatt tttcccgaaa gatttgcaaa
    18901 cggggacaaa gcaaatgacc ccgaacacac tttaccttgg ggaagtaaag atccagaccg
    18961 agaagacttt ttcgggggag atttgcaagg tgtgatggat catttggatt acttgacgga
    19021 ccttggcatc aatggcatct atttttgtcc catatttaaa gccacatcca atcataaata
    19081 cgatacgatt gattattatg aggtggatcc cgcttttggc gataaaaaac ttttaaaaaa
    19141 cttgattgat gaagcacata aaagagggat acgtatcatg ttcgatgctg tattcaatca
    19201 tatgggtgtc cattcaccac agtggcaaga cgttctcaaa aacggagaaa aatcgatcta
    19261 taaagactgg ttccacatcc gttttttccc agtagattct tatcagatga ctgacttacc
    19321 agaaactgct gaaaatatcc catatgatac ttttgctttt actccattca tgccgaaact
    19381 aaatacagca aatccagaag tgcaaaaata tttgctggat atcgcaactt attggataaa
    19441 agaattcgat attgatggat ggcgtcttga tgttgctaat gaagtcgatc atcatttttg
    19501 gaaaaaattc agagaagcag ttacagaaat caaaccagac atctatatcc ttggagagat
    19561 ttggcattcc tctcaagctt ggctgcaagg ggatgaattc catgcagtga tgaattatgc
    19621 atttacagat tcgattaaag attattttgc aaaaaagaaa atcacagctt cacaaatggt
    19681 cagtggaatg aatcatcagc aaatgcttta cagagatcag gtgaacgaag gaacattcaa
    19741 cttattagat tctcatgata ctgccaggat cttgaccctt tgccaaggta acaaagaatt
    19801 gatgaagtct gtgatggcct ttatgtttct tcaaaaaggg tctccttgta tctattacgg
    19861 gacagaaata gggatgactg gtgaagatga cccggattgc cgcaaatgta tgatatggga
    19921 gaaggaggaa caagatctag agctattcgg ttttataaaa gaacttgttt ctctaaggaa
    19981 acagttgtct aagattattt ctgaaggttc tactcagtgg ctaatagtaa atgatcgtga
    20041 agataagctc tatttcacac ggaaactaga gggacagatc atctatgttt actttaatca
    20101 gtcaaaagaa ccttgggtag tagaacagga aaatgaggtg attcttagtc aaaattgtca
    20161 gttgttagaa gacgggaaag cagagatacg acaatacgga tttttgatca tgtgttgaaa
    20221 actgttaccg gtaacaacaa gaaagcgttt gcaaatgaag atttgtgccc attataataa
    20281 gggtgtaaca gcacgttgaa tacaaaggag gaaatgaaga tgaaaaaaag aagtgtgaaa
    20341 cagttgggtt tagggttaat ctctttagga atcttaggcg gattagcggc ttgtggcaat
    20401 ggtaactctt cagcagaagg ggaagccgat gaaggaaaaa cgttaacagt ttccgttgat
    20461 tctggatata aagattatat aaacgaaata aaaaacactt tcgaaaaaga aaacgatgtg
    20521 aaaatcaagc tagtagaaaa ggacatgttc gatcagttgg aatcattagc gttagatggg
    20581 cctgctggaa agggacctga cgttatgatg gcagcatatg accggatcgg tgcgttagga
    20641 cagcaaggac atctggcgga agtaaaactg ggaaatgaag cagcttacga tgaaacagac
    20701 aaagcccaag tgacttatga tggaaaaatc tacggagaac ctgcagtaat cgagaccctt
    20761 gttttgtact ataacaaaga tttagtcgat acagcaccat caacgtttaa agatttggag
    20821 aatctatcca aagacagtcg ttttgctttt gagtcagaag caggaaaaaa tacaggattt
    20881 ttagctaaat ggactgactt ttattattcc tatggtttaa ttgcaggtta cggtggctat
    20941 gtatttggtg atgatggcac aaatccttca gatatcggtt tgaataatgc aggtgctgta
    21001 gaaggaatct cttatgcaac agattggttc aaaaatgttt ggccaaaggg aatgcaagac
    21061 ataaaaagtg caggagactt cgccagccag caattcatgt ccaataaaac tgcagcaatc
    21121 attgacggtc catggcaagc gcaaacatac aaagaaaaca atatcaatta tggtgtagcc
    21181 aaaattccta ctctaaataa tggacagcct tatcaaccgt ttggcggagg aaaaggatgg
    21241 gtagtcagca attatgctaa aaacaaagac ctctctcaaa aatggcttga ttatgtaacg
    21301 aatcaggaaa atcaagaaaa attctttgaa atggtaaatg aaatccctgc aaaccaacaa
    21361 gctagggaaa cagcaaaggc taaaaacgat gaattaacaa cagctgtgat tgaacaatat
    21421 gaaacagctc aagcgatgcc aaacatccca gaaatgggcg aagtttggac aggtgcagaa
    21481 aacttgatgt tcgatgcagc atcaggttca aaaacaccaa aagaatccgc agatgaagca
    21541 gtaaaaacaa tcagtgaagc catagaacaa aaatactgat taaaatgata accgtccatt
    21601 tttataaaaa atggaacagg tgcttcagat aaaagtggca agagcgagat ggaaaattat
    21661 cttgctcttg ttgccattat cttaaaagca gtttgcggtt tagcaagttt agagggggca
    21721 ttcagatgca gcaagataaa acaagcaata tcaacgaaaa aaaaatcacg ctttgttcac
    21781 ttattccagg gatggggcag tttgtaaatg gccaagcttt taaaggtatc gtttttttcg
    21841 ccatattcgt cctattcatt ggtcagatgc ttctgggcgg atttcatgca ttggtaggat
    21901 tggttacttt gggaagtata ccaatggaag atcattcatt atttattttg attgaaggca
    21961 ctttgcaatt catcattaca atcattttct tgatttttta cggtttgaat cttttagacg
    22021 cttatcgagt agcgaagatg cgtcaagagg gcaaaaaaat caatcggacg atgaaggaag
    22081 tcatacataa tataggtgat gaaggttttc cttatctatt gacacttccc gcttatcttc
    22141 tcatgatttt tacgatcatt ttcccagtcc tagttacatt gttcactgca tttactaatt
    22201 atgacttcaa tcatattcca ccagccagtt taattgattg ggtaggggct aaaaactttt
    22261 ttagtatctt ctttttaagc tcatatcgta atactttttt tgcagtcttt tcgtggaccg
    22321 tgatttggac tttatgtgca acaactttgc aaatcaccct tggtattctc acagcggttg
    22381 ttgctcacca gaaatttatt aaattcaaac gtattttcgg tatcattttc ttattaccat
    22441 gggcggtccc agcttttatt actatcatga gtttttcgaa tatattcaac gatagcgtag
    22501 gtgccatcaa tactcaagtg attcctttca taaaccagct tccttttgta gatatcagtg
    22561 caatctcttg gaaaacggac ccgaactgga cgaaagtagc aattatctta gtgcagggct
    22621 ggttaggatt tccctatatc tatgtgatgg ttacaggaat cttacaatcg atttcagaag
    22681 acttatatga agcagcaaaa atcgacggag caaatgcatg gcagcgcttt tcaaacatca
    22741 cgcttcctgc tgtcttccta gtggcagcgc caacatttat tacacagtat acggggaatt
    22801 tcaataattt ctccatgatc tatctgttta acaatggcgg tcccggaagc gtaggcggag
    22861 gagctggttc tactgatatt cttatttcct ggatctataa acttacgact ggaacttctc
    22921 cgcagtattc tatggcttca gcaatcacgt tgattatctc cgtcatcgtt atctgtgtgt
    22981 cattgctggt atttaaaaag acaaatgcgt tcaatatggg ggattaacaa tgaaaaaatt
    23041 tcattcgagt gctcgattcc aaaaacgact ttctcacttt tttacttact tttttctttt
    23101 aatactgtca gtcattatca tctatccgct tttgatcaca gcaagtactg ccttcaagtc
    23161 aggaaatatt gcagctttta gtttagatct ttcaagtgac tggactttag gaaactttac
    23221 tcggctattc agtgaaacac tttacgggac ttggtataaa aatacattga tcatttctgt
    23281 cctaacaatg atcgtacaag tgactgtagt aactttggcg ggctatactt atagtcgcta
    23341 tcgttttatc ggccgcaaat caagtttgat gttcttttta gtgattcaga tggtaccgac
    23401 tatggcagca ttgactgcct tttatgtcat ggctttctta ttagaagcgc tcgataagta
    23461 ctggttcttg acaatgatct atataggcgg tggaattcca atgaatactt ggctgatgaa
    23521 aggatacttt gacactgtac caatagattt agatgaatct gctaaacttg atggggcagg
    23581 gcatttcagg atttttttcc agattgtatt accgcttgtc aaaccgatgc tcgccgttca
    23641 agcattatgg gcttttatgg gaccttttgg ggatttcatg ttagctaagt tcctgcttcg
    23701 aacacctgaa aatcagactg ttgctgtcgg cttacaaaca tttatttctg atgtgacaaa
    23761 ccaaagagtc tctctttttg ctgctggggc aattttgatt gcactaccga tttgtctatt
    23821 attcttcttt ttacaaaaga actttgttac cggcctgaca tctggcggaa caaaaggata
    23881 ggagcagagt atcatgcaaa catttccgtt aaactatttt tccgcattac gtacgccaac
    23941 tcaagtgttt gcgggacgca aactattatc atggcctaaa tttttcttga tatttgtatt
    24001 tttagtttcc ttgatggtca tgcctgtgac tcttttttat gccaatcaga tccaagcgat
    24061 tccattagaa caatttctat ccgttcattc gctgatcgat gaacaaggaa cccaaaaatt
    24121 ttctgaactt gaattatctg aaactgggtt acaagcatcg caacaaacga tagctgtgac
    24181 ccctgaaatt ttggtaggtg tgtctttatc tgagaaacaa caatccgatc atggaacttt
    24241 tattgatttt gagaaggaac aatgggtgat ccaacaaaag gataaatctg gtatacgccg
    24301 ctatacgatg aattatagcc catctttcca accagattct gtacgtacgc ccgaagattt
    24361 ccaacgattt ttagaaagag aattttatgc cagtaatcgt ccaacaatca ttttatctta
    24421 tagtttaagt cttggtcttg tcctatttgt catgacgagt ctcattttat tcggggcttc
    24481 ctttttcttg tggatgacaa gaaaaagcca actttcatcc attcatacat tcaaagagag
    24541 tgcaaatttg atgctgaatg ttatgggaat aggttccatg gcagcagcag ttgtagggtt
    24601 tatccacttt gactttatct tgatgctaag tgtccaaaca acgataactg tcttgctgct
    24661 tttatgggtt tttgtcaaaa caagattcaa agataagcgt gtcgaatgac aagtttaagc
    24721 gatttatgcg acaatgaatg tgaaaaaaat gaaaaataaa gtagtttttg aaaaatttct
    24781 gaaaaaatta aacttcttag ttgattcttt tcttttttag tattaacgta atagggtagt
    24841 ttttttaagg aggaaaatca tgttagagaa aactttatac aatcaattat tttcacgttc
    24901 tttctcgctc cctgtcgaag taaattattg ggacggtaca acgaaacaat acggagatac
    24961 ggacactcct ccccaaatca aaatcaatat ccatgaagaa atcccaatga aagagattac
    25021 aagtaacgct tctttggcac taggagaagc ctatatggat catcgaatcg agatcgaggg
    25081 aagtatccaa gcattgatca acgatgctta tcagcaagca aacagctttt tacgaggaaa
    25141 agattatcta aaatggctgc caaagaaaga aaaacatacg aaacaaaaaa acaaagaaga
    25201 tatccatgcg cattatgact taggtaacga cttttataaa ctttggctag atccaacttt
    25261 gacttattct tgtgcttatt tcaatacacc tgaagatacg ttggaacaag cacaaatcaa
    25321 taaagtccat catatcttag acaaactctt tatcaaagaa ggcgaaacgt tgcttgatat
    25381 cggttgtggc tggggaacat tgatgttcac tgctaccaaa gagtataatg tcaaggcaac
    25441 tgggatcaca ctaagtgaag aacaatacga tttgattacc aaaagaataa aagaagaaca
    25501 tttagaagat aaatgccgtg tattattaat ggattaccgc gaactaaaag gcgaaacgtt
    25561 tgatcatatc acaagtgtag gtatgttcga acatgtaggt tctgaaaatc ttgaaggtta
    25621 tttcaaagtg gtaaaagatt tattgaaacc aaaaggaaca gctttgatcc atggaatcag
    25681 ccgacaacaa ggcggtgcga caaatgcttg gatcaataaa tatattttcc ctggcggtta
    25741 tgtccctggt gttactgaat tagttgataa tattacaaaa aacgatttgc aactgattga
    25801 tttggaaagc ttacgtcgtg actaccaatt gacattagaa cattggacaa agaattttca
    25861 tcaagtccaa gatcaagtca cgaaagaaat gggtgattcc ttctatcgaa tgtgggatct
    25921 atatttacaa gcttgtgcag cttcatttga atcaagtaat atcgatgtga ttcaatattt
    25981 gcttgttcaa cctggaaaca atgacatccc aatgcatcgt gcataattga ggcaaaagta
    26041 ctgtttaaag actatttaaa aagaagctaa gttttatgat gctccagaag tgaacttttt
    26101 tgtctaactt ctaggattgt ctctaaagct tagtttcttt ttctttatat tttcataata
    26161 aaagtttggt tctttaattt ttaaacatga cagaaaattt tttcttcgtt atacttgtaa
    26221 tagaatacta ttttataaca aaggggatcg aatcaatgaa aaaaactgtt atttatacta
    26281 gtttatttgc agctttgttt ttagctgctt gtcaatcaaa taccaatcaa ggcacatctg
    26341 ggaatacgtc ttccagtagc gaacagcgct cttcacaggt atcggactcc tcaacaaaag
    26401 caagcacatc ttcgtctagc cagacaaaaa gttctagtga agcaagtact cagtcagata
    26461 tcaaagtgtc tgcagctcgt gcgatcgaat tgttccaaga taaataccca gatactgcaa
    26521 ttactagttt ggaattagat agtgattggg gatcttactt ctacaagata gaaggtgtcg
    26581 atgatcaaaa agaatacgaa gcaagaattg atgctcaaaa tgaaaaaatg gagacagaga
    26641 atccagaaac tctagatcga gatgaacaaa atggtcaaaa gaaaaaagaa gatggtctgg
    26701 atgttgataa attgatcacg attgaggaag cagcaaaaat cgccgtcgac aaagtaggaa
    26761 gcggtacggc aacagattgg gatttggata aagatttagg tactacttat tgggaagtca
    26821 aagtaaaaga cggcaatcaa agtacatcag tcaagatcaa tagccaaaca ggcgaagtac
    26881 tgtcaactga acaagatgat taagaatttc ttcggaaaac aagagttgat ttttctttta
    26941 gtcatgatac actagacaaa ataacttgtt tgaaggagcg atttacttga gtttagaaac
    27001 gacctttgcc aatcatatct ttgccaatcc tttgatgaat gcatctggtg tccactgcat
    27061 gactacccag gaattagatg aactggctca ttcagaagca ggagcattta tcactaagag
    27121 ctgtacgatc aatgaacgaa aaggaaatcc cgaaccacgc tattttgatg ttcctttagg
    27181 gagtatcaat tctatgggac ttccaaactt aggtttttct tattatctag agtatgctct
    27241 tgcgtatgaa aaagtgcagg aaaaccaaaa tcaaccgtta tttttttcca ttgccgggat
    27301 gagtgtccag gaaaacttgg aaatgcttga gaaaatagaa aaaagtgggt ttaatgggat
    27361 cactgaatta aatttatctt gtcccaatgt gcctggaaaa ccacaactag cttacgattt
    27421 tgaggccact tatgaaacac tgaaagaagt attcagcatc ttttctaagc cgctaggaat
    27481 caaattgcct ccttattttg attttgctca ttttgatcag atggctgata tcttgaatca
    27541 gtttccattg acctatgtca atgctatcaa cagtgtgggc aatggtttgt atatcgatac
    27601 agaacaagaa gcagtagtca tcaaaccaaa agaagggttc ggcggcattg gcggagagta
    27661 tatcaagccg actgctttgg cgaatgttcg cgctttttat acacgtttga aaccagaaat
    27721 ccaaatcatt ggtacaggtg ggattcggac aggacaagat gcatttgaac atctgctatg
    27781 tggcgcaagc atgttacaga ttggtaccga gcttcataaa gaagggccgg aaattttttc
    27841 tagaatcata aaagaattaa cgcagattat gtcagaaaaa ggatatacgt caattgacga
    27901 atttaaaggc aaattacgca ccatttcata gcaaagagac gaaggtcatg attgatcttc
    27961 gtcttttttt ataaaaaaca ctcgtctgtt cattcctgtg caagcgtact cattgtatgt
    28021 tagacgaact tgtgggaaaa taacagaggc aaaaaggagg ataatatgaa tgatgcaaca
    28081 gaattagcaa aagagcttaa agaaaaagaa tttagttttc aagaactagt tacggatata
    28141 cagaaaaaaa taaagaagca aaatcatgaa ttgaatgcgt ttgtcgcgct tgaaccattc
    28201 gagagtaatt cccacgagct attgattccc cagtcagaaa aagggccatt ggctggtatc
    28261 ccatttcctt taaaaatgct cggtcaagaa aaaaagggat ggctagctac ttcaggatct
    28321 cgtttatttg aaacacaccg agcttctcat acatctaatt atgtgcggca agcagaagcg
    28381 ataggtctag ttccttttgg acaaaccaat gcgccagagt ttggtttcaa aaatattaca
    28441 gacccagtca tctatggccc agcaagaaac ccttggaacc ttgaatgtac accgggcgga
    28501 tcaagtggag gagctgctgc tgcagtttca tcaggaatcg ttcctttagc tggtgctagt
    28561 gatggcggtg gttctattag aatacccgct tcgttttgcg gattgattgg gctgaaacct
    28621 tcgcgaggga cgatgccagt tggtccatat gcttggagag gatggcaagg agctgctatt
    28681 gatttcggct tgacagtttc tatgagagac acggaggctt tattttatgg gatgagaagt
    28741 atccattccg gtgcgccata tcaagtatcg ccagttgagt ggcaaacaca tccaagaaaa
    28801 aaacggttga agatagcttt ttgtgttgat tcaccgatcc actcacagat tagtgaagag
    28861 gcagtacttg ctgtaaaaaa agctgtagat ttccttggaa aagaagggca tgatttgaca
    28921 gagattcctt acccactaga tggtcgtaaa ctgatcacgt cttattatca gatgaatggc
    28981 gcagaaacag cagcaatgat ccgctcgatt gaaacaggca tccagcgagc tgtcagaaaa
    29041 gaagaacttg aacccatttc ttggactttg ttccaatatg gtcagaagct ctctgccgct
    29101 acttacatcg attctctgca tgtatgggat gaagcagcaa ttgtcatgga aaacctacat
    29161 caagaatacg atctattcct ttcaccaacg actgcattta ccgcccctaa aatcagcgaa
    29221 gatttgcaaa gtgatgcgat aagagcacag atggaacaca tcagtgattg ttctgaagta
    29281 gaaggtttag acgttgtttc ttccatgttt gaggagagct tgaagatcac accttataca
    29341 cagttggcta atctgactgg acaaccagca atcagcttgc ctactcattt gacaaaagaa
    29401 aacatgcctc taggaatcca atttatggct gccagaggaa gagaagattt gctatttcaa
    29461 atcggctatc tatttgaagc aaatcatcag ttccatttac caaatccatg gaaatagcag
    29521 aaacaaggag aagaagaatg aagaaaaaaa gattgtttgc tctaataact agtgcctttt
    29581 ttattttaag cgcttgcggt acagctgccg ctaatcagga agataacagc tggagtaatg
    29641 tagaaaaaaa gaaagaatta gtagtagcga cttctggcac actgtttccc gcatcttatt
    29701 ataatgatca aaatgaatta gttggttatg atgtagattt ggcaaaagag gtggctaaac
    29761 ggttaaatgt caaaatctcc tttaaagaat acaacgtgga tggacaaatc acttctgttt
    29821 caaaaagaga atcagatttt gctgcgaacg attttggtat cacaaaagaa agagaaaaaa
    29881 aattctccct ttcagagact ttcaaatatt catttgacag tatgatcgta cgaaaaaaag
    29941 atgattcagg aatccactca cttgaagatc ttaaagggaa aaaagcagct ggcgaaccca
    30001 atactagcta tatgcgttta gcggaaaaac acggagctaa attagtcaca tatgacaatg
    30061 ccacgaatga tcagtattta agcgatgtag caaatggtcg aacagatgtt attctaaatg
    30121 attattatct gcagaaaatg gccgtatctg ctttaccaga tatccctgta aaaatactgg
    30181 agaacgttta tttcaatgaa aacgaaagtg gttttttatt gaaagaagga aatgatgctc
    30241 tgaaaaagaa aatcgatggc gtattaaaag agatgaaaga agatgggacg acaaagaaaa
    30301 tatctgaaaa atattttcaa actgatgttt cagtcaaacc gaaagaaacg atggctgagt
    30361 cggtgagtga gtgatgtact ttcctctaat tgatttttcc ctcatgtgga cttcacttcc
    30421 tttcgtactg caaggcttgg tatacacatt agggattggc ttcgtgtcgt ttcttttagg
    30481 aaatctggta gggttgcttt tgacagtttt aggtcttttg gattggctgc ctctgaatgt
    30541 atttattcgt ttttatttat cgttctttag aggcattccc gcactagtcc tgctgtttct
    30601 gttatatttt gggttacctt atcaattaag tgccttaacg gcttcagtca tctgctttac
    30661 gattaccagc agtgcattta ttggtgaaat ctatcgagga tcactagcag gcgtttcttc
    30721 aggtcagtgg gacgctggtt tagcttctgg tttttctttc tttggggtat tgaaatatat
    30781 cgttttgccg caagcttttc gaatctcagt accggcactc agtaacgtag ctatggatct
    30841 agtgaaaggt acttcgttag cagcaacaat cacagtgcca gaaatatttc aaaaagcaaa
    30901 gatcataggc ggtcggacat tcgattattt ttctctctat attttagttg cactgattta
    30961 ttgggtcatt tgtatcggga tcgaaaaagt gcaacagtat ttagaaaaac gttctatgca
    31021 tcagtcaatc tgaaaaatag aaatggtgct agcgtggctg gcaccatttc tattttttgt
    31081 ttcttgtata ttctgcctag tattttaaaa ccatttcatg taaaatgtaa atcgaaaata
    31141 tgttcggagg tagaaaacat gaagaccccg ctaaccatcg aaatagaatc ctcgttatac
    31201 caatattgca tcgagcaagg cggcttggtt gtagaagaag tcacgatgcc tgatgaccaa
    31261 ggaattgtag atacgcttag ctgcttgaca aaatttgatg gaagccgaga atggcgatgc
    31321 tatgagctga aagtgacaaa agcagatttt cgctctacag ccaaactgtc atttgttgga
    31381 cattataatt attttgtttt accaagtaaa ctatatgaag aagtaaaaac tgaaatccct
    31441 acaggaatag gtgttttagt gtatcgtccg tacacggttg aagaggagat gcctgcctct
    31501 ggtacatttc tgattgcgaa aaaagcagca aagaaagagc ttgctgtacc ggaagaagct
    31561 ttgacaaacc gcttcatggc ttctttattc agggaagtaa gaaaagcaaa gcaaatggag
    31621 cgaggcgtaa aatattttcc aacagatcaa ttgtacaaag aattgaaaag acgttcagaa
    31681 aatcaagatc cattttctaa cgaacgatat tatgaacggt ttttagagga aatagctagt
    31741 caggcaatca atgagttgca agaagaagtt atcgcgcttc agcatgatta tgaatatttg
    31801 aggcaacaac agcagatccg tcggttgcct acagagccat tggagtgaaa aatatgtact
    31861 atccttattt aagaggaaaa caatttgacc tattagcatt aaaagaagca ctgtcgcgag
    31921 gattgctctc caataaaatc caaccagtga tcgaaccagt acgtgattcc gctacgctta
    31981 aaaatgtgat cgaattattt caaaaaaagc accatccaat cgctgtgatc caaaatcccc
    32041 aagtaggaca attcaaatta ttcgatcagc atgtgcacag ttggacagtc aaagaaaata
    32101 gctctgtcgt ccctgcacag atcttaacgc cagaaaatct cagtgaagta ctggattcac
    32161 ctccagcgtt tcttgttttc gatggacagc actacccaaa agatacagag gtgtggaaac
    32221 agttggcagg agttgattcg aaatttttga taccagatac tagtcgcttt cgcatctggc
    32281 tgcccgaaaa caaaattgtg atccgtgact cttttcaaac gagaaagcat gttgaaagtt
    32341 atgcagataa aacagatgac ttttttagtg atgactatct ttttttccac gcagacggat
    32401 atattggatt ttctgatttc acgatcgaag gtagccgtta tttcgataaa gggttcccta
    32461 gccgagcgct tgctatccat ctaacgtata ttgatgctta cggcaatata cgcgtgaaac
    32521 attttgtctc tgacagtaac gactctgcta aagaccaagc attgaagttt cttgaagcag
    32581 gagataaaat gaaaaaatgg atcatgcgtc accatcatca actcttgatt acttcaggaa
    32641 tgattgaatt attggctctt taccagcaac aaaaattccc tggactggga gtgttgaaaa
    32701 agtggtcact tatgcatcat ttggaactta ttagtcacct gctagatcat ccaaaagatt
    32761 ggcttaaata ttattcaaaa gttcaggaac tatatgaagt gatccaatga ttagataatg
    32821 taaatgaaaa aggaatagaa aagaggaaac tggatgtata ttcggcaaac aaggaaagaa
    32881 gaagtagaag aagtggtaga aatcattaat gccgcagcgg atttcattgc ttcgcaaggc
    32941 tctcctcaat ggcaaaacgg gcagaaacca acaaaagaaa cgattttgaa cgatattgaa
    33001 agaaaagaaa gctatgtatt aatagtggaa gatcaaattg ctggaacagc tgcattagtc
    33061 tcaagcgtgg atcccgttta cacaaaaata gatggtagtt ggaaagaatc aactggtcct
    33121 tatttatcga ttcatcgtgt cgctttagca gaaagtactc gaggaaaaaa actagcaaaa
    33181 ccgttcttag aacatttgtt gacgattgca gccagcaaaa atatccaaga tatccgtatc
    33241 gatacttatc cagctaatga accgatggaa aagaccatct attctgctgg attcacttat
    33301 caagggatga ttgaatttcc ttttgaacac ggcgaaagaa aagcatatca aataatcatc
    33361 aattgactat aaaatcatct gagcaaagat aaagacatta acactactga aaaaaacaat
    33421 catatgatag ccaagaggtt gtaacagaag tggccagctt caaaaaacaa gaaagaattt
    33481 ccgaaaatgc ttctcaaatt tttgtggaat tttagcgtat tttacgaaat agctgctttc
    33541 tgaaactccc ttcatcgaga ggtcgtgaca tagcttttgt cacgacctct cggcttatcc
    33601 tacgagtatt attcgataca tttatatcaa taatcctttt tttattttat ctgcagcttc
    33661 ttggaacgct ccatcgtttg acacgactaa aagtgagggg atagctttca aacgtttcaa
    33721 ctgtgctaaa tcttctacat aaaccgccag tctggctgaa atctgttcag atgaatctcc
    33781 acgttttttc aggcgcgatt gcaatgtttc tttagagatc gtaataaaga taggacggac
    33841 taccgtcccg aaatactcat ataaatgcca aaaaccattt gctgtcaaaa cggcgtaaca
    33901 atttcctact gtcagtttct ttttaatttc tgctttactg gaaccatatt tatgttgagc
    33961 atattggtcc cactctgcaa actcatttcc cttgatcatc tgattgaatg tattgtcaga
    34021 aacaaaataa taatcctctt gatctttttc cccaggtctt ggtgatcgat tcgtgtaagt
    34081 aatgatcttt tgttccggct gaaaaatagc ttctgcgagt gaagtttttc cagaaccaga
    34141 aggaccgata aatacatagc aataattcat tgtcatttct cctttgatga ttgaacaagt
    34201 gaaaaagctt tggaaaatat ttccaaagct tttaaaagta actttgcttt tttgtatatt
    34261 ataccagcaa gccagtcaca atagcactta aaaagctgac taaagtcgct gttagcaaaa
    34321 ttttcaatcc gtgttttgcg acaagatttc ctttttcttc atttaatccc ttcatcgcac
    34381 cagaaatgat cccgatagaa gagaagttgg caaatgaaac gaggaagaca gatataattg
    34441 ctgttgtacg ctcagtaaaa tggaaattac catttgctaa ttctgtcata gcaacaaatt
    34501 catttgtaac cagtttagtc gccatgatac taccggcatc cactgcttct ttccatggga
    34561 ttccgctgat gaaggcaaga ggggcaaaga tgtaacctag gatctcttgg aaagtgatgc
    34621 cgaatatccc gtggaagata gcattgatca tggcaatcaa agcaacaaaa ccgataagca
    34681 tagcagcaac cgtgatcgcc acatggaaac catcaagaat gtattctccc aacatttgga
    34741 aaaatgtctg ttctttgttt tcttcaatga tcagctcatc ttctttttct tctaatacat
    34801 agggatttac aattgaagcc agaatataac cgccaaataa gttcaaaaca agggcagtga
    34861 tcacatattt agaatcgatc agtgccatat aagatccgac gatcgacata gaaactgtag
    34921 acatagccga tacactcatg gtgaacaaac gtttctcaga taggaagggt aattcctttt
    34981 ttatggaaat aaaaacttcc gattgtccta aaatagcaga agctactcca ttataagatt
    35041 ccaatttgcc catcccgttg attttactta atcctagacc aacagctttc ataaataatg
    35101 gaagaatctt gatatagcgt agaataccga taatagcaga aataaataca attggcatca
    35161 atacggaaag aaagaaaggt gcacttcctt tatttgctac accggcaaaa acaaagtcaa
    35221 caccttgccc agcaaaagca agaagatgat caaacatttt tgccaataca gaaacaaccg
    35281 ctgtaccaaa agttgtacgt aataatacga aaccaagaac aagctgtaaa acgaacatca
    35341 caattagtgg tttcaatttg attgcttttt tgttactgct gatcaaaaaa gcaagcccta
    35401 atgtgacaaa taaaccaata atacctaata aataactcaa ataatcactc ctattccaaa
    35461 ttccgtttta ttttacctgt tttgcaaata aaatactaat ctttagtgaa attttcatta
    35521 cttttttaga gtgaaacgca ttcattttcc attagaaaat aaagcgaaca aaataaaagg
    35581 tttaaaaagt gtaacgtctt agtaaagtct ataaaaaaac gaaactatcg attaagctaa
    35641 tagtttcgtt ttcaacattt ataagtaaac tttttctatt ggtaatatag attttcggaa
    35701 gggtagacag gtgcgcaggt aatatcgatt cctagttttt tcaatgtttg ttcatcattt
    35761 ttattgacca tcacagtaga gtgggcttgt gctccctcaa gttcagctag tttatcataa
    35821 gcaacttgag cgattggatt cgttactgca ctgattgata aagcgatcaa gacttcattg
    35881 atgcttagtg catccaaaga ggaatgaagt gtctttgatt tcaatccttg tattgtttca
    35941 agaactaatg gtgacaataa aggaatgtca tctgcgatat gggctaaatg ttttactgca
    36001 tttaagataa ctgccgctga cgcatccatc aatgaacttg tacgcccggt aatgatgact
    36061 tcatctggta attcgattgc aatcacagca gaaggatctt ttgtaggact ttgcgcttga
    36121 atcttatctg catagtcatg ggcagggact accacgtttc ggtcttcagg acgtaaaccg
    36181 acttcttcca taatcagttt gattcgatta actgcttctt cgtcgatcaa tccttttttg
    36241 aagtcgcatt ctgtttggaa ataacgacgg atgatttctt gtttagaggc ttcttggaca
    36301 acggtatcat ccacgatccc aaatcctaca cgattcacac ccatgtcagt aggagattga
    36361 taaacagatt ctttaccagt aattttttca ataatacgtt tgattacagg aaatgtctca
    36421 acatcccgat tatagttcac agctacttta ttgtaggcat caaagtggaa ggaatcgatc
    36481 atattgacat cttttagatc gactgtggct gcttcataag caatgttcag cggatgtttc
    36541 aacggaacat tccatactgg gaaagtttcg aacttggagt aaccagctgc atgtcctttt
    36601 tggctttcat gatacagttg gttcaaggaa gtagcgagct ttccacttcc tgggccagga
    36661 gctgtcacta caacgattgg tttagttgtt tcaatatact cattttttcc aaaaccatct
    36721 tctgagacga ttttttctac gttgactgga tagtcttcga tttctgagtg tttatagacc
    36781 ttgatgtttc gtctttctag tttattgata aagactttcg tggcaggctg tccactatag
    36841 cgagtgataa cgacactgtt gattaataag ccataaccac gtaattcatc gattaaccgc
    36901 aagatatcca tatcataagt aatcccataa tcaccacgga tcttattacg ttcaatatct
    36961 ccagcataga cgcaaatcag tatttcagct tgatctttca atttttgcag caatttgatt
    37021 ttcgcatctt cttcaaatcc tggaagtaca cgtttcgcat gcttgtcgtc aactagcttc
    37081 ccaccaaatt ctaaatataa tttgtcatag tggttcactc gttccaaaat atacgctgac
    37141 tgctcttcaa tatatttttc tgaatcaaaa ccaatttttt tcactaggtt ccctccttaa
    37201 gtactaaagc attttttcat ttttttgagg attcgtcaac tggaagagcg aaattcctca
    37261 aaaaaacaaa gaaaatggtt taaaccaact tgtctagata gttattcatt ggtatgatag
    37321 tttttgataa ggaaggtgct acatgaaaaa aagacagaaa aagaaaaacg catataaaca
    37381 ctatatccgt tcgattttta ccggttatga aagaatgctt gaagatcccg aattggaaca
    37441 attgacgttt acttatctaa acgaagaaac gcagctaact cgtgatgagc atcagcgcat
    37501 tcatttcaca acaagagatc tgcctagtaa ataaaaaata agataaaggt catcggattc
    37561 gattctgtcc tatcagatgg aaagactagt tgatcgaaaa aaaggctgta ccaaattttg
    37621 gtgcagcctg ttttatggag cattgcccgc tcttttttat aacaattttt tacagaaaat
    37681 ggtactattg aagtattaat aagtgaaaaa cagatgagta agaggaggca agtagtatga
    37741 aagagccaga tacaaatgca aattatgagc cagacgaact agaggagacg aatgcggatc
    37801 aggatacagg acaagaatcg tcttcttttt acgatatcaa agaaaaagca agcatgaatc
    37861 tttcccaatt attagaagac ttacaggctt ttgaaaaagc tgtccgcgaa gaagatatcc
    37921 ctgagatcta ccgtttatac aatggccgat tgaatgaaga attaaaaaga agctcaaatg
    37981 aaaatcacga gatcgatcaa ttgctgatgc gtaagatcca tgatcgtttt ttacaggaat
    38041 ttccttttat ggaacaagta gaaaacatct cgccaacgat gagctattac aaaatcggca
    38101 cttattacca tgatcggccc acggtcggta ttgatgcaag tctgccagaa atttttgttt
    38161 tgcctaagat cgatgaagag tgggagaaat attcccaacc ggagtcaacg gactatgaca
    38221 aaaaaatcaa tgagctagac gcaaaggtga tcactgctca gacagaaatt gaacgtttag
    38281 atgaacaaat aaaagaaatc aatcgacaaa tgactgattt gaacgacaat aaaggattct
    38341 tgaatcgtaa aaaaatcgag gaagaaattc aagaattaga gaagaagaag caagtactat
    38401 ctaatgaaaa attaggctgg cttccttata ttgagacgcc tgaaacaatc cagcagcaaa
    38461 aagaagcatt gaagcaagaa gcaagagcag accagcttcg tgcagccatc gtagaaaaag
    38521 aacaaaggca aatcagacgc tatttcggta gcaaagaggg attcggtcaa gcaatccatg
    38581 aattcttgat gaattatctt ggcaatgaga atcctaataa tcaatctaat gaaggaggtt
    38641 ccgagtatga atgaacacga tgaagcagaa tggacaaaaa atgaagaaca aacagaagat
    38701 catgaagaaa cgaacacttc attggaagaa caaacaagta atcctctgga tgacacagaa
    38761 gaaatcccta tgctagagga aacaaaaata cacgaaaaag aaaaagtaac tccgacgaat
    38821 acgaatactg cagaaaacac ccatgcttac gaaataactg aaaatcccgg gtatttagaa
    38881 ggaaaagagc aagatcatca agatatcgtc agtttgattg aaacagcgga agcagaatta
    38941 gcggatttac gtatgagaaa agatttgatc gaatcagatt ttccacaatt attagaatca
    39001 tatgatcgtt ttatattgaa tgaagaacca atctccacga tcggaaagac aagcctgatc
    39061 gaatacttca cgtatgaatt actgaaaccg ttgaatgata ttggtctgga acggtcaaat
    39121 tcagcttatg acacatggaa aacaagacat ttttcgatca attatacgtt gaatggacaa
    39181 aacgaattag tcttttcctt tgtattaccg accatcgatc aacgatacca agtggaatcg
    39241 atgagtctcc taaacgtatc accagagaca atggaaatca atgtccaaga tgaccgggtg
    39301 ctttctttga tccgacactg gtcagtggat cgagtattct ctgcagggca gatcacgata
    39361 ttcaatcaca aattgaatca attattagcc catgcaagaa cacttggttt taccgttaat
    39421 cagacattac tagacaatac gaagccactt cgtttgaatc ttcagagcga atttgaattg
    39481 acgaatgaag tattggatga tatttttatc gtggcaatga aaaatccatc ctacgatatg
    39541 gaaaagatca acgaaactac ttatcaagta cgattagata aaggacagtc tttaacgata
    39601 acaacaaacg aaaaagatca aacagtcttg tcgatctcat ctggagacta cccacgatct
    39661 gtcattgatt tctttatcaa ttacgaattc ttggtcccat taatggtgag aaagtcctga
    39721 ttggataagt tggattacca gatgtcattc agataattct gttccaaagg acttgcggca
    39781 gttatccaga ataattgtca caagtctttt ctgtatgtac taaatggacg tgaataactt
    39841 gctgatgaag gagggatttc attaggaatg tccacaaata aaatagcaca gcttagctta
    39901 ttgtctgctg cttgtgtagc aggtaggatc gcttttcaat ttattccgaa tttccagcca
    39961 atgactgcta tttttttatt tatagtactt tacttaagct taaaagatgc gttaattgtc
    40021 atgagtcttt ctattgcgat cagcagtttt tattttggtg ttggaccatg ggtcatcggc
    40081 caatggatca gctatactgt tgttctgagt ttgttttcaa tcgtctgctt gagaaaaaca
    40141 gtgcaaagga atctatggtt aaagggagtc tgtttttttc tggcgggcat ggtttacgga
    40201 ataggtatga ccgtttttga tacgctgtta tatcgtttgc ctcaaccttg gatctattac
    40261 cttcaagggg tgtcgtttga tttgatgcac agtataggca atgttgtgtt ctttcttgtt
    40321 tttctgccag tcgtcaaacg tttttacaat cattcaggag gaaaaaatga aaaaaataat
    40381 ttatagtttg cttgcaatga gtgtcagtat actcattttg acaagttgca gtacaaccta
    40441 tacacaatcg gcttccaacc aaaaggagga agtaaaaaca tcgcaaaaag caacaatcac
    40501 gttacaagaa aatggtcaaa acttttcgga aaaagaagtg gtcttcaaaa aaggcgatga
    40561 tttactgagt attttgaaac gaaattttga agtcaaagaa gataacggct tcatcacttc
    40621 gcttgaaggg cactcacagg atgagaaaaa tcaactctat tggatgttta ctgtggacgg
    40681 gaaaagtgct actactggtg ccaaagagat caaattgaat gatggacaat cggtcatttt
    40741 caatttgtct aaactataag tttcataaaa aactgttaaa gggcgaatat tgccataatt
    40801 cagtgagttc agttgatgaa agaaggaaaa taacatggaa aattttaaaa aaagcggaat
    40861 gttttccgta attgctgctc taggggctaa tattctagtt gctatcagca aatttgtcgg
    40921 ctatgcaatc agtggcagtg ccgccatgct aaacgaaagc atccacagta tcgttgattg
    40981 cagcaatcag attttgctgt tattcggtga taaacaagcc aataaaggac agagcgagtt
    41041 gcatcaattt ggtgaaggac gagcaaaata ctttttcagt acaatcgtcg caatgatgct
    41101 tttcttcggc ggtggggcac ttggggtgat ggaagctttt gaaaagttga cgcatccatc
    41161 ccatgaagtg ggtaatccga tcattgttat agcaatactg ttatttggtt tggtaatcga
    41221 aggaagttcg ttacgcgtgg caatgaaaga aatcaaagaa ttgaatactg aaaaactttc
    41281 cacgatgcgc tttttgcgcg aaagtcgaca cagtgaaata ctgattattt ttacggaaga
    41341 cttgtgtgct gtaatcggtt tggtcttggc tttagcagga acaatattga cttatgcaac
    41401 cgggattgct gcatttgatg cgatcagcgg attgctgatc ggtttcttgc tgatgggagc
    41461 cgccattttc ctagcaaaag aattttatag tctgatcatc ggtgaaagcg tgacagcaag
    41521 cgatttatca aaaatcaagt tagcttttga acgtcctgag atcagtcggc tgatcgatat
    41581 aaaaacagtt catttaagtc caacggagat tttagttgca gcaaaagcag atatcgttgg
    41641 taacaaagag aatgaagctt actcggtcat caatgatatt gaaaaaaata tgcgtcagtc
    41701 tcttcctgac aagaagatgt atatatacat tgaaacagat aaatatgacc caaattattc
    41761 tcgtaaataa aacgtttatt catgaattga agcaaaaatg agaaaaatat aatcttgttt
    41821 ttaaataaaa cggctacaat ttagcgaatt tatgttacaa tgagtccgct gtttatttgt
    41881 tgtaattaaa ggagatattt taatgaacaa aaagaaaagt gcgccatggc ttcaagcttt
    41941 gcatgcagtg atgccacttt gtataagcta tataccagta ggattagctt gcggagtatt
    42001 gttgcaaaaa gttggatttg atcctctctt atcaggactg ttatcgattc tggtcttttc
    42061 cggtggcgcg cagtttttag ttgcatctat gcttacgacg caagcatcat ttgcaacgac
    42121 attattgatg gttttctttt tagaattacg ctatacatta ttaggagcaa gtcttgccgg
    42181 ttttatgaaa aaagaaaagc ggacgttttt ggctgtcttt tctcaatcac tgaatgatga
    42241 aaactatgcg gtgaactact taaagttttc aactgaccct tcctggaata aaagaaaggc
    42301 tttatatgtc aattggttct caatgactgc atgggccggt agcaatatga tcggtaattt
    42361 tttcggctcg gtgattcgag tagatgctga ccttgtccat tttgctttga cggccatgtt
    42421 cattttcatg tttatcatgc agatgaaaag cgcactactg atctttacag gtttattctc
    42481 aggcgtatta ggggtcgtat tcatggtatt attccaaaat actttcggat tgattgcagc
    42541 aacagtcatt gcttcatttg ccggatttat gctggagaag gcattcaaaa aagaaaaagc
    42601 taagaaacta agagaccgcg gtaaagatgt caatgagccg ctcttcaact tcccggaaca
    42661 ggaggccaat caccatgact aaactatatt tgctactttt agttgcttgt ctgttcatcg
    42721 tggcatatat cccgcgcttg ttcccgatgc tttattttac tcatcgaaaa gtgccatcct
    42781 ggttcagtga ctggatgaaa tatgtaccgg tggcattatt tgcggcgtta gcttttaaag
    42841 atgttttcat tactcatgaa catctggata ttgcttggaa tatcaaaata gctgcaatga
    42901 ttctagtagc aggcgtggct tacaaaacac gttcgatggc gttgtcagtt ttgacaggac
    42961 ttgcgtctgt ctttttgctg tctatgcttt aaactgtttt attttattaa aaacagagat
    43021 tgcgacataa gttgttcaaa ctgatctatc cgaactgcat ttgcaacagt caattctgga
    43081 tgcaagttta ttcgtaggga taaaaaagat tagtgttggc ttatgatgca agctcttttt
    43141 tattttagga attagagagg gatttgtcct tctatgattt tttgctataa tagggaagat
    43201 gtttatttta tgaaagaata gttgagggaa tttaaatgaa taaaaaattg atgcaaggaa
    43261 cattttggct aacctttgct aatcttctat gtaaagtttt aggggtagtc tatttgattc
    43321 cctggctaag tatgatgggg aacaatcaag atggtatgct tgctacgaca ttgtataatg
    43381 ttggctatct tccttatgga ttgttcctga tgcttggtac agtcggattt ccaaatgcga
    43441 ttgcaaaaaa agtagctgta gccacaaaag aaggagataa cgctgcttgt cggctgattt
    43501 ttagaagtac gatcaatatc atgtttgtca tcggtatcgc ctctgctgca ctgatgtatt
    43561 tatttgctcc tttactagcc ggaatcagtc cgattgctaa cgttaataat gggatactag
    43621 ctatcagaag tttatgccca tctttgattg ctatcccaat tcttagtgca atgagaggat
    43681 atttccaagg aaaaaatgat ctgcggcctt atggaacttc tttgattatc gaacaggctg
    43741 tgcgagtgat cgttatcttg gctggtactt attacttgcg cgtattgact gacggaacca
    43801 tattagaagc tgtattgatc agtacagtcg cttctttctt cggtgggtta gctgcgatta
    43861 tccatatgta tatcgtcgga cgcagaaaag acttcttcga acttaaagat tttttgattt
    43921 ccagccgtta tttcgaaaga gaaaaccgat ctgcctctgt ctctatcatt cgggaaacac
    43981 taccgtttat ttttgtcggc tcagtcatta ctatcgttca aatgatcgac caagtaacga
    44041 tgaaaccact gcttcacttt ttccgtccag agatcgcaga tcagcaactg gaattcttat
    44101 tcagccgtgc ttctgtcaat ccaaataaat tgacattgat cctgatctcc atggtaggaa
    44161 cagtagctat aagcagcttg ccaatattaa gcacgatgaa gaaaaaagat cggatacaaa
    44221 tcgaaaaaac agtaggagat agtttttcga ttgcgttatt gattcttctt ccttcattga
    44281 ctggaatgtc tttattagct ggaccactat acacattgtt ttttggctat gatcctgaaa
    44341 gtgtcggata tttccaaatg gctttgctag cttctttgtt tttctcttta ttcacgatcc
    44401 tatctacgat gcttcaatct ttgaatcacc atcgttttgc gatccgatta actgtagaag
    44461 caattatcct gaaagtcatc ttccaagtaa tcggattagg attagttggc ggatacggga
    44521 tgagcttatc aaatggctta gcattcggta ttgtctttgt tcgcggctat cactttatgt
    44581 gcaaagaata ccgaatagca cctttagcaa agatcagcaa ttttttctta aaaactttcc
    44641 gctccaccct gattatgctt gcagtatgtt ttgccgtttt ctttttgctg aatcagcgat
    44701 tgtcaatgga atcaaaaagc tatgcaatga tttattgtgt ggctgtagga agtattggcg
    44761 gcttagtatt tttattttct caattcggaa aaaatgggtt tcgaatgttg aaaaatgtta
    44821 aacatcatag cagaagcaaa gaataggtta actaatcgtt ttttgctgtc acttgaaaaa
    44881 cggcaactcg tatgattacg cttgtcgttt ttctattttc caattttagt ttcatatcta
    44941 atcttactta tgatatagtt ataaggtctg gaaaaattct ttgaaacgag gaaattaaac
    45001 acatgaaact tgcagtagtt acagatagtt cggcatatct gccggaacgt atcaagaacc
    45061 atcccgactt attcgttatt ccaatccctt tgattatgga tgggaaaatt tataacgaag
    45121 gaatcgatat cgaagcggat gaatattatg gacttttaaa aaacagtaaa gattttccta
    45181 ctacttcaca acctgtcgta ggggaagttt tgggtttata tgaggaattg aaagagaaag
    45241 ggtatgacac gattttaagt atccatcttt cttcagggat ttctggcttt gtcaacacat
    45301 tatttgcgat gaaagatgac atcagcggag taacggtcat tccttatgac tctaagatca
    45361 ctagtatgcc gatgggacac atggtagaag ctgccttaga cttaaatgat gaaggtcagt
    45421 cccttgatga aatacttgca catatcgatc gaatccgaga taatacctat gcttatctga
    45481 ttgtagatga tttgaacaat ttagtacgag gaggacgttt gacaaatggt gcagcgctga
    45541 ttggcagctt attaaaaatc aaaccgatcc tgacattttc tgaagggaaa attgttttat
    45601 tcgaaaaaat ccgttcaagt aaaaaagcat ttgctcgagc tgaagaaatt attggtagaa
    45661 gaaataacga aatacaaccg ccagtcaaac tttacgtgat acatgcaaat aatttagaag
    45721 tagcgattgc ggagaaagag aaattgcaga aaaaatatcc aaatgcagaa atcgaaattg
    45781 gtcatttcgg accagtcatt ggtacacatt taggtgaaaa agccatcggc attgccattt
    45841 cagcgcaata attcctttta ttcaaaaaac ataccaaaaa tat
//
LOCUS       DASHHB010000019        44696 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.019, whole genome shotgun sequence.
ACCESSION   DASHHB010000019 DASHHB010000000
VERSION     DASHHB010000019.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44696)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44696)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44696
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.019"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..377
                     /locus_tag="U2K71_001125"
     CDS             <1..377
                     /locus_tag="U2K71_001125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="glyoxalase"
                     /protein_id="HEM7315227.1"
                     /translation="KIHLNVTNLTKERQFLNHVLGLKVHNEEEGIHVLNEGDFHVGLT
                     EASGGAIDLPTDEVIGLDFLKFMIDTESMEGLIAHLQQLSVDFFLDKKHTVLTIYDAI
                     GIEWWFVSEPKEKNKTDIRCDA"
     gene            586..1611
                     /locus_tag="U2K71_001126"
     CDS             586..1611
                     /locus_tag="U2K71_001126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEM7315228.1"
                     /translation="MYPHTKQLILKKMEEGFYPGVAVAFIKENQVETFTAGFAQVVPE
                     KQLMTEDLLFDAASLTKVVATTSLVLRLLEDGKIELDQPLHKYLPEFENQTITLRHLL
                     THTSDIKTWIPNRDKLSQEELIAAYLKLEPGEQLGKEVKYTDAGTILLGLMLEEIYQE
                     TAVEIFQEQVLEPLGMMNSLFLPFPSKKIVPTEQLPSGEVLRGQTHDPKARVLAEHAG
                     NAGLFIDLKDSITFVQMYLNKGKIRNGRFLEEETIDSLLQDQTPKKNGYRSLGWDLKK
                     DVEDGHAILFHTGYTGTFFLIDIKTQEALIFLSNRVHPVDHRAEYLICRDEIVENFLK
                     EKAVLPK"
     gene            1650..2600
                     /gene="manA"
                     /locus_tag="U2K71_001127"
     CDS             1650..2600
                     /gene="manA"
                     /locus_tag="U2K71_001127"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase, class I"
                     /protein_id="HEM7315229.1"
                     /translation="MQEPMFLKPVFQEKIWGGSRLRSVFGFDIPNDKIGEDWAISAHP
                     HGVSVVENGEFKGKRLDELWSEHKELFGHPSEPVFPLLIKILDAEDDLSVQVHPDDAY
                     GMKHEGELGKTECWYIIDAEPGAEIIYGHHAKTREELAEMIKDGRWDDLLKKVPVKKG
                     DFFYVPSGTIHAIGKGIMILETQQSSDTTYRVYDYDRKDAQGQTRELHIQQSIDVTTV
                     PAKTPELQIKEVRKGNSSIVTYLETEFFNVYEWDIKGITSFKKQAPYTLMTVIEGAGD
                     LVVDGKTYPLEKGTSCIIPNGVSEWAVQGELAIIASEPGK"
     gene            2694..4397
                     /locus_tag="U2K71_001128"
     CDS             2694..4397
                     /locus_tag="U2K71_001128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM7315230.1"
                     /translation="MEYKWKANNSSRVSMKQLRKAGHHLNEMIMPNLSLFIAWGILSL
                     ATKGVSGDLHTTLEEVCHWMIQLLLPILISYSGGKSVGGQKGAVAGAIASLGLIARSN
                     APQIVGAMIIGPLAGIVYEQFVSRFHVKFKAGYEMLVSNLLVGLTGSVICIIGIVFIE
                     PVLNSVHLLLASVVSWLVTMNLLPLVSLILEPLKVLFFNNAINHGVLTPLGIEFSQTV
                     GYSSLFLMEANPGPGLGILLSILCFAEKQEKVNASGALMIHAIGGIHEIYFPFVLLRP
                     YLFLAVMAGGASGTLIFQWLDTGLAAPVSPGSLLVIFANAPYPQLAGVSIGIAISTFV
                     TFICAAILLKLPNKEKENKTVKERGEILEPSIIHSVIFACDSGMGSSAMGAALLKKKL
                     AAEKIDLSVGYSSIYELNNQVNQLVIVQHELAGLAREKVPQSQIYVLDHFLETDILLS
                     RIKEAIQFPKREEKKEIADITEQKTSPANIVFLYKNNRRGSQTMGMTILSQLAKKAKK
                     QFVISKESIEKIKIQEETIYIAQRELAVTEQLEEKIPRLVLVEHWVATNEYEKLVKGE
                     I"
     gene            4399..6432
                     /locus_tag="U2K71_001129"
     CDS             4399..6432
                     /locus_tag="U2K71_001129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BglG family transcription antiterminator"
                     /protein_id="HEM7315231.1"
                     /translation="MYLSPREKQLLAEFLSSPSPVSIQKMMNLLKVSKRTIYRELDNL
                     EVSLKSVNASLKKAARGSFVIEASEEVLEQLKENTDGKSNDLSTAKRQHAILAELLLT
                     TEPLSLAHFMESYRISNTTFYADIKQLEESIRQLPLEIIRNQGYEIEGPEKYRRLLTA
                     NVLELEINEYELFHSISFDASLNYFFRFVDPQHLSLARKVIGEELIQKKTNLSDRKLE
                     HLVLMLTLTMERVTKNHLLTNETYNSLANKELLQTAKRLFSRIASETKQLYPVNEIVF
                     FAGLLNDFTNSFDQNFFEETFDTQLAYLVKQLIQEVSEETNVHFYEDETLYKMLLTHL
                     SGVFSRAVLQEETLTNPILEKIMVQYEEVANAIRKAVERIFQEKKLSEEEIAYMVLHF
                     ANSLERSPKESNISIAGFSPSGLASTSMLELRLKRYFPFIQQIHFYRIADLKKIDLQE
                     EYDVVISTSILPGYGGKYILVSPLLLEDEVKQLKEAFSHIEKKKRQKITYNSNNEWMI
                     EDTYEETMHFMDQINQLLGRFFIQELDNPETISETIRSLFPYFPEGLVTDEEVVHRRL
                     LKRYEQAPIGIPHTNMGLFHTSSSLISKPIFCIFNLKHPLTIEGMDRQPVDLSRMLVM
                     LAPSPIQEKDGILLGKISGAIIMNDLNTEIFRSGNKEIVYQLLAKILIEEIKK"
     gene            6443..6883
                     /locus_tag="U2K71_001130"
     CDS             6443..6883
                     /locus_tag="U2K71_001130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM7315232.1"
                     /translation="MIQLPAEKILLDQSYQNWKEALTEIGQWMTQQGGIKPAYIQSMI
                     ERQEKASVYIGNFVALPHGTDKDEEVVEEGIYLVQVPDGVDFGTKEEPKIATLLFVVA
                     LKKEHQLAVLQDLAFFCSDIDQVMALSDAETIEEVQMIFEHGHI"
     gene            complement(7083..8210)
                     /locus_tag="U2K71_001131"
     CDS             complement(7083..8210)
                     /locus_tag="U2K71_001131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315233.1"
                     /translation="MSLLTFERNLLLKNKVNFLFPLLLVVLFAFPLFFDHKLAYTEFD
                     ELNHNYEEMQRLIETLKEDENEKEFVESLEKSNKLIEAILHAKNTGNVQQMVEATYHY
                     EKDILDRLISGQRQGIPIIEQQKRVELLRYMKEHQIQRYSIFDLPAHLSLANYYENIF
                     SGMISSFLILCITALFLSSIISYEKRKQVISLVNLLLDSMVKKHSIRFTIYYGAAMLS
                     LVMPFLIVSILVIIKNGLGDFRYPVGTIIGQEIRILPMYEYLFQSFLFLLLWVLFLST
                     ISFLLSALFEHSLVNLLGTLLCLFLAEYRLFSSIGWIESISHYLPTSYVDFQNVIIGG
                     DIFSPLASEQVTFMNGILTLGIWSIVLLFIGMGTIYIKKSY"
     gene            complement(8213..8890)
                     /locus_tag="U2K71_001132"
     CDS             complement(8213..8890)
                     /locus_tag="U2K71_001132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315234.1"
                     /translation="MTLLIKNMNMKRGKKRVIRQLNLLVESGEVAALIAPNGFGKTTL
                     LNGIAQLLPTTYDQLSLHNISPTAYTDYRRSFFFLESTNQLSDYLSAVDYLTLIKYYW
                     QSKVECQTVFQRLKMTEYLTLPIGKMSLGMKQHVLLAAYLISDSSVMLFDEPLNGLDP
                     GSIDILNSLIRQWQKEGKTILLSSHAIANIQSICSKACFLKNGEIVTESSNMEEILAI
                     YRKLYEV"
     gene            9154..10920
                     /locus_tag="U2K71_001133"
     CDS             9154..10920
                     /locus_tag="U2K71_001133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannitol transporter subunit IICBA"
                     /protein_id="HEM7315235.1"
                     /translation="MERTIPKNNISLKARVQKLGSTLSSMVMPNIGALIAWGVLTALF
                     IPDGYLPNEAFATMVSPMLTYLIPLLIGYTGGKVIAGDRGSVVGAIATMGVIVGTDIP
                     MMLGAMIMGPLGGYAIKKFDQLFQKRIKSGFEMLVNNFSAGLIGFGLALLGFSAIGPV
                     VDALTQAMAKGVEIILSAHLIPLTSIFIEPAKILFLNNAINHGILTPLGTEQVAEAGK
                     SILFLLEANPGPGLGVLLAFTLFGKGAAKSSAPGAMIIHFLGGIHEIYFPYVMMKPLL
                     FLSVIGGGMSGSFIFQLMDAGLRAPASPGSIIAILAMTPFNSYLPVILGVIVAAAVSF
                     GISAVILKADAKDTNEDFEKSVQETKAAKQASKGAQGIPQGATFSGVQKIIFACDAGM
                     GSSAMGASILRKKVQEADLPQTVTNQAISQLTDDSNTLIVTQVELQERAKQKAPSARF
                     VSVENFLNSPRYDEIIQELSSKQKTSVSEIENEPAEEFLSANIKQIKEILLVHDDRSG
                     SATMGMTVLKDILDKNHLTIPLRKVNIQDLKDEPDSLIVAKESLAEKVQMAAPQSIHL
                     SVNSMVNPQKYESIAQYLKQTA"
     gene            10935..11372
                     /locus_tag="U2K71_001134"
     CDS             10935..11372
                     /locus_tag="U2K71_001134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM7315236.1"
                     /translation="MQSLPFDMIELNRSFKTKEEAIRYCGQMLVEAGCVDEEYIDAMV
                     DRDKMLSVYMGNFIAIPHGTDEAKKYVKKSGICVVQVPDGVDFGTEEEEKIATVLFGI
                     AGVGDEHLQLVQQIALYCSDMDNVVQLADALSKEEVTENLAIA"
     gene            11385..12536
                     /locus_tag="U2K71_001135"
     CDS             11385..12536
                     /locus_tag="U2K71_001135"
                     /EC_number="1.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol-1-phosphate 5-dehydrogenase"
                     /protein_id="HEM7315237.1"
                     /translation="MRAVHFGAGNIGRGFIGEVLAANGFEITFVDVNHTIIDALNERK
                     EYEIELADESKEKITVKNVKGINNQTNPEKVIQEIAEADLVTTAIGPNILPFIAELIA
                     KGIQEREKEGSTTSLDIIACENMIGGSAFLEKEVYKYLPETSFTDKYIGFPNAAVDRI
                     VPLQHHEDPLFVQVEPFKEWVIDDSQRKNKKIQLKGVLYVDDLEPYIERKLFSVNTGH
                     ATVAYTGALLGYQTIDEAMQDALVVAQLKSVLQETGSLLIAKWGFDAEQHHAYIEKII
                     HRFQNKNISDAITRVARTPLRKLGYQERFTRPVRELQEHNLTCPHLTATMGIIFNYYD
                     PEDEQSRQLHEMKIHENLEQLIQEVTGINDPKTIGNIKQNVNRYAKQVA"
     gene            complement(12598..13296)
                     /locus_tag="U2K71_001136"
     CDS             complement(12598..13296)
                     /locus_tag="U2K71_001136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase"
                     /protein_id="HEM7315238.1"
                     /translation="MANYELNIAHLYGNLMNTYGDNGNLLMLKYIAEKMGVSCHTEIV
                     SIHEPFDADKYDLVFFGGGQDFEQLIISKDIQKKKDSLINYIENDGVMLAICGGYQLL
                     GHYYMGANGEKIQGISALDHYTLSQENNRFIGDIVIHNEEFNETYYGFENHNGRTFLG
                     EGERPLGKVVQGQGNNGEDQSEGVIYRNVFGSYFHGPILARNENLAARLIRLALEQRY
                     GKELDLPYLEGQTA"
     gene            complement(13289..14641)
                     /locus_tag="U2K71_001137"
     CDS             complement(13289..14641)
                     /locus_tag="U2K71_001137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase family protein"
                     /protein_id="HEM7315239.1"
                     /translation="MGIRSHFAIAVGKTSQWALKTFFKGGSSLPGKLALKVDPHILDS
                     LAKDYQVVVVTGTNGKTLTTALTVNILRQQFDEVLTNPTGANMVQGIVSTFLQAKPKK
                     GQKKFAVLEIDEASLSKVTEYMKPELFLFTNIFRDQMDRYGEIYTTYKMIVDGAAKSP
                     NATIISNGDSPIFNSIETVNPRKYYGFDHEEDREQMAHYNTDGVLCPNCHHILHYKMI
                     TYANLGKYYCPNCDFKRPELDYRLTDMVRMDNVSADFVIDGHEYGIEVGGMYNVYNAL
                     AATAVAEYFNVDPDKIKQGLGYDEKVFGRQEVININGKKCTLVLVKNPVGINQVIDMI
                     GLAPYPFSLVALLNANYADGIDVSWIWDGNFENFADMDIPAVISGGDRHTDMTLRLKV
                     AGIPENKLTETKELPEVIEKIKELPTEHVYILATYTAVLQLRKELAAQGFIKGGMNRG
                     "
     gene            complement(14864..16264)
                     /locus_tag="U2K71_001138"
     CDS             complement(14864..16264)
                     /locus_tag="U2K71_001138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkH family potassium uptake protein"
                     /protein_id="HEM7315240.1"
                     /translation="MHVDLFFRRLQRRGQKFAANHFSSIQIIVFYYILMTVLSLVLFY
                     MPIFREPDSHVSFVDMLFMAISTVSVTGLSTFDINSVFNDNGIILLEILFQVGGLGIM
                     MISTAFVIFSKRRITLKQRQLIMTDMNQPRLSGIVRLIRITFAILIWFQLLFGTFFSI
                     YFYYRGYFDRWRDAVFYGFYQAISAVTNSGFDVTGDSIKPFAHDYFFLILIMFLIFVG
                     GIGFPVLMECREWLLYKRSNAKLPFRFSLFTKLAVLAFVILFVSGTVLIYLLEKDHLF
                     QDSNMSVKWINSMFYSITTRNAGLQIHDLGDFQITTLIIFSLLMFIGCSPSSVGGGIR
                     TTTVAIIGLYLYSFLKSEDNINIFGRRIDQDDVRKSVVVFMLSLGMCFFCIVFLSATE
                     EQTLISIIIEVTSAFGTTGLSLGITGDLSVVGKITIATLMFIGRIGMLYTLMLFVPKE
                     TRDLGYEYPSEKIIIG"
     gene            16425..17162
                     /locus_tag="U2K71_001139"
     CDS             16425..17162
                     /locus_tag="U2K71_001139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="epoxyqueuosine reductase QueH"
                     /protein_id="HEM7315241.1"
                     /translation="MINAQQIVEKMKNQKINYDTVLKKMIMQWEKEEVKPKILIHTCC
                     APCSTHTLEFMSQYAEITLFFSNSNIHPKSEYLRRLHEQKRFVDQFNEKTDHHVALIV
                     DEYRPNEFTKMVLTNHLENEKEGGARCSACFNMRLDLVAQKAQELGYDYFGSALTISP
                     KKNAALINQIGMDIQKIYGTQYLPSDFKKNKGYERSLQMCKEYDIYRQCYCGCVFAAK
                     QQGCDLKEINREAKAYLKTSTVVFEEN"
     gene            17287..17766
                     /locus_tag="U2K71_001140"
     CDS             17287..17766
                     /locus_tag="U2K71_001140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A-agglutinin attachment subunit precursor"
                     /protein_id="HEM7315242.1"
                     /translation="MRKVAPILKPIAVLTISTIVATTTGIVNSSSYRAPSSSSSTVEE
                     TTPTTSVSTSEPTTTESLNETTTTSTWDDTTTSSSYDETYTTDSSSETSNTVPDGTPS
                     DSTNPSTSPDTSASSETIPSSTTVDDNTQNDDAARTAQQNQDSGNTQTYNQQAGDSQ"
     gene            17763..18530
                     /locus_tag="U2K71_001141"
     CDS             17763..18530
                     /locus_tag="U2K71_001141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315243.1"
                     /translation="MTFLLDLINGVHKFLGYLDISPKYLNRGYTILSVIPTLYLLRIV
                     YGLWQNQNYLQFFLYGLAFLVLVYFTVLNVFYYFLDKNTKADVTQLFVKYLPDEAFNI
                     QTEESIDNGNSIDKVNTQEVMVSYDEDYQLKLAENMRYLIGNGEIKTNELGRMDGFLV
                     DRNTLYPYYYVKKTGEKEYVLNIGRSYSDMEKVGTIRLDSTEDTLEPVGLFIVGGDFV
                     KDGLRYHEPYRLKLIAKKAQSQEEPVVYSRSQRRRHS"
     gene            18760..19329
                     /gene="lepB"
                     /locus_tag="U2K71_001142"
     CDS             18760..19329
                     /gene="lepB"
                     /locus_tag="U2K71_001142"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HEM7315244.1"
                     /translation="MKEFLKNWGILILVLAAILLARVYVFTPVTVNGHSMDPTLSDGQ
                     RLISSKISNYERMDIITTKEPGDEERMIVKRIIGMPGDTVKMENDQLTINGKKYDEPY
                     LDEFKKEFSEDKLQGEYAYSSGFQAQAESSSTFTSDFEYTVPKGKYLVLGDNRLISKD
                     SRMFGLVDKDMIQGKVVFRYWPLSEIKIM"
     gene            complement(19433..20035)
                     /locus_tag="U2K71_001143"
     CDS             complement(19433..20035)
                     /locus_tag="U2K71_001143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM7315245.1"
                     /translation="MTKNIFDSIANHYDSPDRQALAKIIREELTTYLPRDSHQKVLLD
                     YGGGTGLVSLPLAERFKELIIADASETMLKMAEEKIQAADLKNVRTIHADASVEFPAV
                     QANLILLSLVLLHIPDTENILTKLYEILAPGGQLIIVDFDKNEQISHPKVHNGFSQEE
                     LNNRLEKTGFVSATSHTFHRGEKLFMNKNASLFLSISQKA"
     gene            20141..20610
                     /locus_tag="U2K71_001144"
                     /pseudo
     CDS             20141..20610
                     /locus_tag="U2K71_001144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325408.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
     gene            20716..21300
                     /locus_tag="U2K71_001145"
     CDS             20716..21300
                     /locus_tag="U2K71_001145"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HEM7315246.1"
                     /translation="MAQLFFKYGAMNSGKTIEILKVAHNYEEQNKPVVLMTSGIDTRD
                     GLGVVSSRIGLKRNAMPIFEETDVFDVIQKMESKPYCVLIDEAQFLKKKHVIAFTRIV
                     DELDIPVMAFGLKNDFRNELFEGSKYLLLYADKIEEMKTICWFCHKKAIMNLHYIDGN
                     PVYEGDQVQIGGNEAYYPVCRKHYFHPSMITEGE"
     gene            21303..22376
                     /gene="prfA"
                     /locus_tag="U2K71_001146"
     CDS             21303..22376
                     /gene="prfA"
                     /locus_tag="U2K71_001146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003128515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HEM7315247.1"
                     /translation="MYDQLQAIEDRYEELGELLSDPEVISDTKRFMQLSKEEANTRET
                     VEVYRRYKEVVQGITDTEELLGEKLDDEMAELAKEELSELKKEKEVLEEKIKILLLPK
                     DPNDDKNIIMEIRGAAGGDEAALFAGDLFNMYQKYAEAQGWKTEVMEANVTGIGGYKE
                     VIMMITGDNVYSKLKYESGAHRVQRVPSTESQGRIHTSTATVVVMPEAEEVEIDIEDK
                     DIRTDIYHASGAGGQHVNKTASAVRLTHLPTGIVVAMQDERSQIKNREKAMKILRARV
                     YDKIQQEAQSEYDANRKSAVGTGDRSERIRTYNFPQNRVTDHRIGLTIQKLDQILAGK
                     LDEIVDALIMYDQTSKLEAMQNG"
     gene            22369..23208
                     /gene="prmC"
                     /locus_tag="U2K71_001147"
     CDS             22369..23208
                     /gene="prmC"
                     /locus_tag="U2K71_001147"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HEM7315248.1"
                     /translation="MGKMTYREVLSRASSFLEDHGKEAYSIQFLFLERKQWKKLDWLL
                     HMNEEISEEEQRLIETDLRLLLADHPPQYLLGYADFYDHRLKVTEATLIPRPETEELV
                     EWCLDETPGVPLEVIDIGTGTGAIAISLKAARKNWHISAVDLSEEALEVAKENAQKEG
                     TKISFYHGDTLGPVMDQQFDVIISNPPYISRNEWELMDESVRSFEPKMALFAENDGLA
                     IYEKIAVEAPSVLKSNGKIFLEIGFRQGEAVKKIFQQAFPDKKVAIKKDLFGNERMIQ
                     VYG"
     gene            23201..23380
                     /locus_tag="U2K71_001148"
     CDS             23201..23380
                     /locus_tag="U2K71_001148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315249.1"
                     /translation="MVEVVDEKFFICFFIIKKHLFDKDVNDFLVYQQVYPLLTNRLST
                     NSGKLEDKLRQNKNV"
     gene            23402..24427
                     /locus_tag="U2K71_001149"
     CDS             23402..24427
                     /locus_tag="U2K71_001149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonylcarbamoyl-AMP synthase"
                     /protein_id="HEM7315250.1"
                     /translation="METKRYDETELQEAAEALKEGELVAFPTETVYGLGANALLPNAV
                     KKVFSVKGRPQDNPLIVHVASFEQVKEYVDNFHPAAEKIVKNFWPGPLTLIFKIKKDT
                     LPSVVTGGLSTAAFRIPDNKKTLEVIELSGVPLVGPSANTSGKPSPTTADHVYHDLQG
                     KITGIIDDGATRIGVESTVLDLSDPTAMPMILRPGAVTKEQIEAVIESPVAIDQHLVK
                     ENETPKAPGMKYKHYSPDTRVLMVRDGDWSTAVQWAKNKKIRAGVIASPEIADQVRTD
                     TAAVYMYNDNSVEAAAKGLFAGLRGLDEPTLGLDLIFVQVYPETGLGNAYMNRLKKAA
                     GQNYFEK"
     gene            24618..25862
                     /locus_tag="U2K71_001150"
     CDS             24618..25862
                     /locus_tag="U2K71_001150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HEM7315251.1"
                     /translation="MVDYKTFDPDLWAAIAKEEERQEHNLELIASENFVSEAVMAAQG
                     SILTNKYAEGYPGHRYYGGCEFVDIVENLAIDRAKELFGAKFANVQPHSGSQANTAAY
                     LALVEPGDTILGMDLSAGGHLTHGSPVNFSGKTYHFVAYGVDPTTEVIDYNVVRILAR
                     KHQPKLIVAGASAYGRTIDFAKFREIADEVGAKLMVDMAHIAGLVAAGLHPNPVPYAD
                     ITTTTTHKTLRGPRGGMILTNDEALAKKINSAVFPGIQGGPLEHVIAGKAVAFKEALD
                     PAFKEYSEQIIANAKAMVKVFNQAIGTRVISGATDNHLMLIDVRELGINGKEAESILD
                     SVNITVNKNSIPFETLSPFKTSGIRIGTPAITTRGFKEEDAVKVAELVVKALQAKDDN
                     AQLDEVKTGVRELTEKFPLHKK"
     gene            26015..26962
                     /locus_tag="U2K71_001151"
     CDS             26015..26962
                     /locus_tag="U2K71_001151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM7315252.1"
                     /translation="MTDRISLEKEAVAFSDANAPHPRIYELAPEEGRVLLEEVQESPV
                     NKYSVDIEDLMVDTGEWGKLNIRLVRPEGNMDKLPVIFYIHGAGWVFGSAQTHDKLIR
                     ELAVRTNSVVVFPEYSRSPEAKYPTAIEQNYAVLQQLNKLAKSKNLDLENLIVAGDSV
                     GGNMATVMTILTKERKGLPISKQLLYYPVTNADFDTDSYHEFGENYFLTREGMMWFWD
                     QYTTNEEERAEITASPLRANINELNDLPSAMILTGEADVLRDEGEAYARKLRDAGVPV
                     TQVRFQGIIHDFVMVNAMDQTKATRAAMALSTSWIKKKE"
     gene            27096..27725
                     /gene="upp"
                     /locus_tag="U2K71_001152"
     CDS             27096..27725
                     /gene="upp"
                     /locus_tag="U2K71_001152"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013774406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HEM7315253.1"
                     /translation="MGKFQVIDHPLIQHKLTIIRDKNCGTKVFREVVDEIAMLMAYEV
                     SRDMPLEDIVIETPITETTQKTLSGKKVAIIPILRAGIGMVDGILELIPAAKVGHIGL
                     YRDEETLEPHEYFVKLPEDIDARQLFVVDPMLATGGSAIMAIDALKERGASNIKFVCL
                     VAAPEGVKALQEAHPDIDIYTASLDEKLNEHGYIVPGLGDAGDRLFGTK"
     gene            complement(27864..28106)
                     /locus_tag="U2K71_001153"
     CDS             complement(27864..28106)
                     /locus_tag="U2K71_001153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315254.1"
                     /translation="MKMATFGKFDTTIVPSEIGEKFKINTHVMFTIHGKSQTGTISKQ
                     LKNSAVVEIDETAENANLMTQSKGVVIVNYKELKKI"
     gene            28227..28299
                     /locus_tag="U2K71_001154"
     tRNA            28227..28299
                     /locus_tag="U2K71_001154"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:28260..28262,aa:Thr,seq:cgt)
     gene            complement(28418..29056)
                     /locus_tag="U2K71_001155"
     CDS             complement(28418..29056)
                     /locus_tag="U2K71_001155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM7315255.1"
                     /translation="MNNILGKKIELKKGILGTIVVVLGLLGLLFAPSIISIFSLTITG
                     FIAFTLVFGKKDTKKMFSKPVAPVKNIAKYFFINVVISAAVSVFLQQILKWGLTGNPI
                     NEAFSPLLFIILPIMILGEELFSVYFLAIFSSKFSIPVASFLSAIIFGFIHYSTYDNG
                     NILHTVAHILLIQGVARLLFNQAAIKSNSIITSWAVHVIFDFTAILLVVLFS"
     gene            complement(29179..29811)
                     /locus_tag="U2K71_001156"
     CDS             complement(29179..29811)
                     /locus_tag="U2K71_001156"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEM7315256.1"
                     /translation="MIFPKVNEASLNKGSFFHIYENEDFELYNILSQKEVIENQIICH
                     PIIYKSIFKYAFFASNDFQRMEITDCIFENCDFSGCILTKSMLHRVKFVNCKLTGTKL
                     MESYIGNTLFENCKMDYVNLSGSNIKESTFEHSVLSSADFVDCSLTKIMFSTNDLENA
                     SFFDTDLKNIDLSSNSFEMIEVRSEHLKNCTLNQLQALGFARKFLQIKVV"
     gene            30162..30461
                     /locus_tag="U2K71_001157"
     CDS             30162..30461
                     /locus_tag="U2K71_001157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEM7315257.1"
                     /translation="MSKSEEIENMVLKLYDLVLNPEIKEKERILLIDAKTGLEKGQYY
                     PKVINNLERSLRPLAIRGELSKPVSPFYMEISTIGKFEKELGRGMASAPITFGHL"
     gene            30956..33121
                     /gene="acm"
                     /locus_tag="U2K71_001158"
     CDS             30956..33121
                     /gene="acm"
                     /locus_tag="U2K71_001158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="collagen-binding MSCRAMM adhesin Acm"
                     /protein_id="HEM7315258.1"
                     /translation="MKKCRNIILSMLFIITNITSLIPVHVYADAGRDISSNVTSLTVD
                     PTNITDGGNIKVKFSFDEKKQNIQPGDYLWINWPSEGNIRGEGFQKEIPLMIENKNVG
                     TLTVRKDSAQVVFNENIKNLDSVEGWGQFEIQARNVTDTGEENTGPFTVTSGDKTATV
                     NVTKPASGSSSSVFYYKTGDMLPEDTKHIRWFLNINNNGTYVEQPVKISDEIQSGQRL
                     DPSTFEINQIHLGEQKVYRGEEGIQQFLQDFPSATFNFSVTDNYIEITIPKNFVNLRK
                     IMVSYKTIIENPEQINFENHSEAWFKEFNKPAVDGESFNHTVKNISASGGVNGTVRGE
                     LKIFKYINDTEIGIPNVTFELRRADEQPIQGQSSILLTSNEQGEASIKGLQVGDYVVK
                     EKEAPNWIDFDPLSSNELKFSINENDTEGVSLPIYNKKKVTNITATKIWNGGTTPRPS
                     IYFKLFRASTNNWEPVPDAETKRLDNGITSVTWEDIQQYDDSGNEYTFKVQEVDQNGN
                     DYVPSGYQKIENGLVVTNENKEVISVSGQKTWEDNENQDGKRPTTITVNLLADGQPIQ
                     HKEVSEKDDWRYRFTNLPKYRDGQEIIYTVTEDNVPEYSTTIDGYNIKNSYTPEKTNI
                     SIPGSHITPWKNFPEKTEREKIRNLFSQKLQIFAHTGESSEQRSNYISKRAVPKQITN
                     KSKSILPKTSSKKSSFAVIIGLLIVTICAGIFLQKYKKV"
     gene            complement(33393..33878)
                     /locus_tag="U2K71_001159"
     CDS             complement(33393..33878)
                     /locus_tag="U2K71_001159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl-tRNA synthetase associated
                     domain-containing protein"
                     /protein_id="HEM7315259.1"
                     /translation="MSRATEQEAYELLKKLGISFQKVDHPAITSVKNVPFSLPGPQVK
                     NLLLKSRKGKQIYLVILPDEKHADLKQLAEQLKEKRLSFVSEEQLPDLLGVPAGTVTP
                     LALMHDKEKKIQVVIDAAIDRQDTVGFHPNINTTTLIMDFSDFEKILVYLEHLPVYEN
                     L"
     misc_binding    33968..34313
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            34358..34999
                     /locus_tag="U2K71_001160"
     CDS             34358..34999
                     /locus_tag="U2K71_001160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM7315260.1"
                     /translation="MEYLIEVMPQLLVGALTTLKLFLFTLIGSLPLGIVLALGLSLHF
                     KPLRYLLQTYVWVMRGTPLLLQLIFIFYGLPTIGIVFDRFEAALIAFILNYAAYFAEI
                     FRGGIQSIPQGQYEAAQVLQLSPFQTVTKIVIPQVTKIVLPSVGNEVINLVKDSSLIY
                     ILGLGDVMRAGKIAMERDATLLPLLGVAIIYLTLTGILTIVMKQVEGKLNYYR"
     gene            35018..35653
                     /locus_tag="U2K71_001161"
     CDS             35018..35653
                     /locus_tag="U2K71_001161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM7315261.1"
                     /translation="MIELKKVNKAFGEKKIIKDLNLIIPDGKTLAIVGPSGGGKTTLL
                     RILAGLESSDSGEFYLDGVPFNPFELKMKEQVVGVVFQDFQLFPHLNIFENITLAPKM
                     VLKQTKEEYTKKAEELAASLGISDLLNIYPYQLSGGQKQRVAIARALAMNPRVLAYDE
                     PTSALDPELRQQVERLILSLKEDGVTQIIVTHDLVFAENVGDQLLKVSPIH"
     gene            35671..36486
                     /locus_tag="U2K71_001162"
     CDS             35671..36486
                     /locus_tag="U2K71_001162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM7315262.1"
                     /translation="MKKTSFLFALIAGLLLFAGCSNKKTSADNWATINEEKKIVIGLD
                     DTFVPMGFRDKDGKLTGFDIDLAKAVFEQYGITVDFQPIDWSMKEFELNNGTIDLIWN
                     GYSKTPAREKKVQFTKPYMENDQVLITPKESGITNFGQMNNKRLGAQNGSSGYDVFTK
                     QPEVLKEIVKNQDAVLYDSFNEALIDLKSRRVDGLLMDKVYADYYLKQRNELEDFNIT
                     KGSYESEDFAVGARKSDSTLVKKINEALDQLEKTGEFQKISDKWFGEDVTPRN"
     gene            36805..38241
                     /locus_tag="U2K71_001163"
                     /pseudo
     CDS             36805..38241
                     /locus_tag="U2K71_001163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289243.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
     gene            38430..40877
                     /locus_tag="U2K71_001164"
     CDS             38430..40877
                     /locus_tag="U2K71_001164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEM7315263.1"
                     /translation="MAQKKRPTKKRKKTKKTKKQKQQQEQMLTIGVGFALILFAVFGF
                     LKLGFLGILIANGFRIIAGNTYQILCLLLAVLGFWIVIKNTEFSIGKNRRWFGGILFY
                     FGILLLLHAHLFGKLHTGEPNIMGTTWDLLASDIKQSQVDNNVGGGMIGAILYHFTYF
                     LIAQPGSYLAAVLLLAGGAFLFSNLEGYQLLNGIQSIGERVQELLEGDPAKQARKQAA
                     KEERMKQRAEAKEARRLAAKEAAEKEAAEYEKKKAVSQQRENTADEWQEPENTEPEQL
                     SFVPIDSFQENIHPANLEKPVPDTPKQTNTAEGFSDELPEDDGTSLEFEIEAEQENQD
                     YELPTVDLLDSIPTVDQSDEYKKIEKNIGVLEQTFQSFGVDAKVVKASLGPSVTKFEV
                     QPAVGVKVSKIVNLTDDIALALAAKDVRMEAPIPGKSLIGIEVPNGKISMVSFREIIE
                     AQPNHPDKLLEVPLGRDVSGRVQTADLSKMPHLLVAGSTGSGKSVAINGIITSILMRA
                     KPHEVKLMMIDPKMVELNMYNGIPHLLTPVVTNPRKAAQALQKVVQEMEERYEKFAAT
                     GVRNISGYNEFVQQKNLENGTKHPTLPFIVVIVDELADLMMVASNEVEDAIIRLAQMA
                     RAAGIHMILATQRPSVDVITGIIKANVPSRMAFAVSSGTDSRTIIDSNGAEKLLGRGD
                     MLFLPMGENKPIRVQGAFISDHEVERVVQFVTDQQEAHYEEKMMPTDEVETTGAPEQP
                     QDELFEEAKALVVEMQTASISLLQRRFRIGYNRAARLVDELEAHGVVGPSEGSKPRKV
                     FIEQEETSETLPDDNDM"
     gene            41107..42174
                     /gene="rlmN"
                     /locus_tag="U2K71_001165"
     CDS             41107..42174
                     /gene="rlmN"
                     /locus_tag="U2K71_001165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEM7315264.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(42367..43470)
                     /locus_tag="U2K71_001166"
     CDS             complement(42367..43470)
                     /locus_tag="U2K71_001166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM7315265.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            44022..44570
                     /gene="aac(6')"
                     /locus_tag="U2K71_001167"
     CDS             44022..44570
                     /gene="aac(6')"
                     /locus_tag="U2K71_001167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEM7315266.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT        15269 a         7677 c         9125 g        12625 t
ORIGIN      
        1 aaaaaatcca tttaaatgtt actaacttaa caaaagaacg ccaatttctt aatcatgtac
       61 ttggtttgaa ggtacataat gaagaagagg gcatccatgt attgaatgaa ggagatttcc
      121 atgttggttt gacagaagct agcggaggcg caattgattt gcctacagat gaagtgattg
      181 gacttgattt tctgaaattc atgatcgata ctgaaagcat ggaaggactg attgctcact
      241 tgcagcaact atcagttgat ttcttcttag acaaaaagca cacagtactc acaatctatg
      301 atgctatagg aatcgaatgg tggttcgtca gtgagccaaa agaaaagaat aaaactgaca
      361 ttcgatgcga cgcttaactt tttctttctt aacgattgtc gcatgtctcg tcattttatg
      421 aacggtcata ctatcgctta ttctagagat agaaagagaa aatagtatac cgggggaaac
      481 tgcgcggttt ttcgcgcagt ttttttgtgt aaaaaaggtt gattcctcat agattaagct
      541 ttaagcttgg actgtaagaa tctgatagaa aagcaggaga aaaagatgta tccacacacg
      601 aaacagctta ttttgaaaaa gatggaagaa ggattttatc caggtgtagc agtcgccttc
      661 ataaaagaaa atcaagtaga aacatttaca gccggctttg cccaagtcgt tcctgaaaag
      721 cagctgatga cagaagatct gttgtttgat gcagcctcac tgacgaaagt cgttgcaacc
      781 acttcattag ttttacgtct attagaagat ggaaagatag aactcgatca accgcttcat
      841 aaatatcttc ctgaatttga aaatcagaca atcactttgc gtcatttact gacacatact
      901 tctgatatca aaacatggat accaaacaga gataagctat ctcaggaaga attgatcgcc
      961 gcttatttga agctagaacc aggtgagcaa ctaggaaaag aagtaaaata tacagatgcc
     1021 ggtacgattc ttcttgggtt gatgctagag gaaatttacc aagaaactgc agtggaaatc
     1081 tttcaagaac aagtattgga accattagga atgatgaaca gcttatttct tccttttcct
     1141 tccaaaaaaa tcgttcccac agaacaactg ccttcgggtg aggttttgcg tggacaaact
     1201 catgatccga aagcaagagt tttggctgaa catgcaggta acgcgggtct ctttattgat
     1261 ttgaaagaca gcattacttt tgtccaaatg tatttgaaca agggaaaaat ccgtaatggt
     1321 cgttttttag aagaagagac gattgattct ttactgcaag atcaaacacc gaaaaaaaat
     1381 ggctatcgtt ctttaggatg ggacttaaaa aaggatgtag aagacggtca tgcgattctt
     1441 tttcatactg gttatacagg tacgtttttc ttgattgata taaaaacaca agaagcgttg
     1501 atatttttgt caaatcgcgt acatcctgtc gatcatcgtg cggagtatct catatgtcga
     1561 gatgaaattg ttgaaaactt tttgaaagag aaagcggtat taccgaaata atgctataat
     1621 agctatgaaa aatgagagga gtttgaatta tgcaggaacc tatgttttta aaaccagttt
     1681 ttcaagaaaa aatttgggga ggaagtcgtt tacgttcagt ctttggtttt gatatcccta
     1741 acgataaaat cggagaagat tgggcgatca gtgcgcatcc tcatggtgtc agtgtagtag
     1801 aaaatggcga attcaaaggg aaaagattag atgaactatg gtcagaacac aaggaattgt
     1861 ttggccatcc ttcagaacca gtgtttccat tattgatcaa gatcttagac gcagaagatg
     1921 atctatctgt acaagtacat ccagatgatg cctatgggat gaaacatgaa ggtgaattag
     1981 gaaaaactga atgctggtat atcatcgatg ctgaacctgg tgcagaaatc atctatggtc
     2041 atcatgcaaa gacaagagaa gaactagctg agatgatcaa agatgggcgc tgggatgacc
     2101 ttttgaagaa agtaccagtt aaaaaaggtg atttctttta tgtaccaagc ggaacgatcc
     2161 atgctattgg taaaggaatc atgattttag aaacacagca aagttctgac acgacttacc
     2221 gcgtgtatga ctacgacaga aaagatgcgc aaggacaaac aagagagctg catatccaac
     2281 aatcgatcga tgtaacaact gtccctgcga aaacaccaga attacaaatc aaagaagtcc
     2341 gaaaagggaa ttcatcgatc gtcacttatt tagaaactga gtttttcaat gtatatgagt
     2401 gggatattaa aggaatcaca tctttcaaaa aacaagcccc ttatacattg atgacggtaa
     2461 tcgaaggagc aggagacctt gtcgttgacg gaaaaacata tccattagaa aaaggaacaa
     2521 gctgtattat tccaaatggc gtttctgagt gggcggtcca aggtgaacta gcaatcattg
     2581 cgtctgaacc aggcaaatag ttcccagata gctgaaagca gcagacggtt tttcgtttgc
     2641 tgctttttct acataaaatc cgtccatcaa taaatagaag tctggtgaaa aagatggaat
     2701 acaaatggaa agcaaacaac agcagtcgcg tgtctatgaa acaactgaga aaagctggac
     2761 atcacttaaa tgaaatgatc atgccaaatc taagtctgtt tatcgcttgg ggaatcttgt
     2821 cattagcaac aaaaggtgtg tctggcgatt tgcacaccac attagaagag gtatgccatt
     2881 ggatgatcca gctgctttta cccatcttga tcagttattc aggcgggaaa tcggtagggg
     2941 gacaaaaagg ggcagtcgcc ggtgcaattg cttctttagg gttgatcgct cgttccaatg
     3001 ctccacaaat cgtaggagcg atgattatcg ggccgcttgc cggcatcgta tatgaacaat
     3061 ttgtttctag attccatgtg aaattcaagg ctggatatga aatgcttgtc agcaacctac
     3121 tagtaggact gactggtagc gttatttgta taattggaat cgtttttatt gaaccagttt
     3181 taaatagtgt tcacttgttg ctcgcctccg ttgtgtcttg gctagtcaca atgaatctgt
     3241 taccacttgt cagtctgatc ttagaacctc taaaagtact atttttcaat aatgcaatca
     3301 atcatggtgt attgacacct ttaggaatcg aattctcaca aacagtcggg tactcttcct
     3361 tatttttgat ggaggcaaat cctggacctg gtttagggat cttactttct atcctttgtt
     3421 ttgcagaaaa acaggaaaaa gtaaatgcga gtggagcatt gatgatccat gcgattggcg
     3481 ggattcacga aatctatttt ccatttgtct tgctgcgacc gtatctattt ttagctgtaa
     3541 tggctggtgg ggcaagcggc acgttgatat ttcaatggtt agatacggga ttagctgcac
     3601 ctgtatcgcc tgggtcacta ctcgtgattt ttgccaatgc gccttatcca caactagctg
     3661 gggtaagtat agggatagca atcagtacat ttgtgacctt catttgtgca gcgatactat
     3721 tgaaattacc aaataaagaa aaagagaaca aaacggtcaa agaaagggga gaaatcttgg
     3781 aaccatctat cattcattcc gtcatttttg cttgcgactc aggtatggga tcaagtgcta
     3841 tgggagccgc cctgctgaaa aagaaattag cagcagaaaa aatcgatctt tctgtcggat
     3901 acagctctat ctatgaattg aataaccaag taaatcagct ggttatcgtg caacatgaac
     3961 tagcgggtct tgcaagagaa aaagtacctc aaagtcaaat ctatgtactg gatcactttt
     4021 tggaaacaga tatcttgctg tcacgaataa aagaagcgat ccaatttcca aagcgggaag
     4081 aaaaaaaaga gatagcagat atcacagaac aaaaaacgtc gcctgctaat attgtatttt
     4141 tgtataaaaa caacagacgc ggctcacaaa ctatgggcat gacgatttta agtcaattag
     4201 caaaaaaagc gaaaaaacag tttgttatct caaaagaatc aatagaaaaa atcaaaatcc
     4261 aagaagaaac gatttatatc gcacaaagag aactagctgt aactgaacag ctagaagaaa
     4321 agatccctcg gctagttcta gttgaacatt gggtagctac gaatgaatac gaaaaactcg
     4381 tgaagggaga aatataagat gtatttatcg cccagagaaa aacaattatt agcagaattt
     4441 ctcagtagcc cgtcacctgt atctatccaa aaaatgatga atttattgaa ggtcagtaaa
     4501 cggactattt accgcgaatt agataatctt gaagtctcat tgaaatcggt aaatgcttct
     4561 ttgaaaaaag cagctagagg ctcatttgtc attgaggcat cagaagaagt attagagcaa
     4621 ttaaaagaaa atacagatgg gaaaagtaat gacttatcca ctgctaaacg acaacacgcg
     4681 attttagccg agttgttact gacgacagag ccgttgagtc ttgctcattt catggaatca
     4741 tatcgcatca gtaataccac tttttatgca gatatcaaac agttggaaga aagtattcgc
     4801 caactgcctt tggaaatcat tcggaaccag ggatacgaga tcgaaggacc ggaaaaatac
     4861 cgccggcttc tgacagcgaa tgtattagaa ttagaaatca atgaatatga actttttcat
     4921 agtatttctt tcgatgcatc actgaattat ttttttcgat tcgttgatcc gcaacatcta
     4981 tctcttgcac gcaaagtaat cggggaagaa ttgatccaaa aaaagaccaa tctaagtgat
     5041 agaaagcttg aacatttggt tttgatgctg acattgacga tggagcgtgt gacgaaaaat
     5101 catttactga caaatgagac atataatagt ctggcaaaca aagaattact acagacagct
     5161 aaacgattgt tttcacggat cgcttctgag acaaaacaac tttatccagt aaatgagatc
     5221 gtcttttttg caggattatt aaatgacttc acgaactcat ttgatcagaa tttctttgaa
     5281 gagacgttcg atacacagct tgcttattta gtaaaacaac tgattcaaga agtaagtgaa
     5341 gagacgaatg tccattttta tgaagatgaa actttgtaca aaatgctgtt gactcattta
     5401 tctggtgtgt tttcaagagc cgttttacaa gaagagacac tgaccaatcc gattttagaa
     5461 aaaatcatgg ttcaatacga ggaagttgcg aatgcaatcc gtaaagcagt agagcggatt
     5521 tttcaagaaa aaaaattatc cgaagaagag atcgcttata tggtccttca ttttgcgaat
     5581 tcattggaac gaagtccgaa agaaagcaat atcagtattg caggattttc gccaagtgga
     5641 ttagcttcta ctagcatgtt ggaactacga ttgaaaaggt attttccctt catccaacaa
     5701 attcatttct atcggattgc tgatttaaaa aaaatcgatt tacaagaaga atatgatgtt
     5761 gttatttcta catctatttt accaggatac ggtggaaaat atattttagt ctccccgcta
     5821 ctgctagaag atgaagtcaa gcagctaaaa gaagcttttt cgcatattga gaagaaaaaa
     5881 aggcaaaaaa ttacgtataa cagcaataac gagtggatga tagaagatac ttatgaggaa
     5941 acaatgcatt tcatggatca aatcaatcaa ttactcggtc gtttttttat ccaagaactg
     6001 gataatcctg agacgatttc cgaaacgatt cgcagcttat ttccttattt tcctgaagga
     6061 ctagtaacgg atgaagaggt ggttcatcgc cggctgctta agcgatatga acaagcacct
     6121 atcggtattc ctcatacaaa tatggggtta ttccatacat ccagctcatt gatttctaaa
     6181 ccgatctttt gtattttcaa tttgaaacat ccgttgacca ttgaagggat ggatagacag
     6241 ccagtcgatt tgtcccgtat gttagttatg ctggcgcctt cgccaatcca agaaaaagac
     6301 gggattcttt taggaaagat cagtggagca atcatcatga atgacttgaa caccgagatc
     6361 ttccgctcag gaaataaaga aatcgtttat cagttgttag cgaaaatcct gattgaagaa
     6421 ataaagaaat gaaggtgaaa agatgataca gctaccagca gaaaaaatat tgctcgatca
     6481 atcctatcaa aattggaaag aagcgttgac cgagatcggc cagtggatga cccaacaagg
     6541 gggaatcaaa ccggcatata tccaatcaat gatcgaaaga caagaaaaag cgagtgtcta
     6601 tatcgggaat ttcgtagcct tgccacatgg gactgacaaa gatgaagaag tagtagaaga
     6661 aggaatctat ttagtccaag tgcctgatgg tgtggacttt ggaacaaaag aggaaccaaa
     6721 aatcgcgaca ctcttatttg tagtggcttt aaaaaaagaa catcagttag cggtgctcca
     6781 agatttagcc tttttctgtt cagacatcga ccaagtaatg gcgctcagtg atgcggaaac
     6841 cattgaagaa gtccaaatga tttttgaaca tggacatata taaccaatgt tcacagtaaa
     6901 aaaagctgcc ttgcatcaag taataacttc aaccaagata aaaataagag gagctatttt
     6961 tgttgggagg ttcttattac tgcggatgca agcttttgaa aactatttat tctagaaaac
     7021 cgtttatttg aaatagggga cggtgacaag gtttttgtca ccgcccctat tatttttgtg
     7081 ttttaatagc tcttttttat atagatagtg cccataccta tgaataataa aacgatactc
     7141 catataccaa gtgtcaagat accattcatg aaagtaacct gttctgaagc tagtggcgag
     7201 aaaatatctc ctcctatgat cacattttga aaatcaacat aagaagttgg caagtaatga
     7261 ctgattgatt ctatccatcc aatcgaagaa aacaagcgat actctgctaa gaaaagacaa
     7321 agaagtgtac ctaaaagatt gactagagaa tgttcaaata aagcagatag aagaaaactg
     7381 attgtactta aaaagagcac ccacaacaac aggaacagaa agctctggaa aagatactca
     7441 tacatcggca ggattcgtat ctcctgacca atgatcgttc ctacaggata acggaagtct
     7501 cccaaaccat ttttgattat tactagtata gagacgatca aaaatggcat cacgagactt
     7561 agcatagctg caccataata aatggtaaaa cggatgctat gtttctttac catactatcc
     7621 agtaatagat tgactagaga gattacctgc tttctttttt cataagagat gatggaagaa
     7681 agaaacaagg cggttataca taaaatcaaa aaactagaaa tcattcctga aaaaatattt
     7741 tcgtagtaat ttgctaaaga gagatgcgct ggcaagtcaa aaatagaata tctttggatc
     7801 tgatgttctt tcatataacg gagcaactcg acacgttttt gttgttcaat gattggaatt
     7861 ccttgtcttt gaccagaaat cagtcgatcc agtatatctt tttcataatg ataagttgct
     7921 tcgaccattt gctggacatt ccctgtgttt tttgcatgta agattgcttc gatcagtttg
     7981 ttgcttttct ctaaactttc cacaaattct ttctcattct catcttcttt caacgtttca
     8041 atcaaacgtt gcatctcttc atagttatga ttcagttcat cgaattctgt ataagcaagt
     8101 ttatgatcaa aaaaaagtgg gaatgcaaag agcacgacca acaatagagg aaataaaaag
     8161 tttactttgt tttttagtaa gagattgcgt tcaaatgtaa gtaaagacat agttacacct
     8221 catataattt acgataaata gctaaaatct cttccatatt tgaggattct gtgacgattt
     8281 ctccattttt tagaaaacat gctttagaac aaatcgattg gatattcgcg atagcgtggg
     8341 aagaaagaag aatcgtcttc ccctctttct gccattgtct gatgaggcta ttcaagatat
     8401 caatacttcc tgggtctaat ccgttcaacg gttcatcgaa taacatgaca gatgaatcac
     8461 tgatcaagta agcagcaagc aagacatgtt gtttcattcc tagagacatt tttccaatcg
     8521 gcaaagtaag gtactcagtc atcttcaatc gttggaaaac agtttggcat tctactttgc
     8581 tctgccaata gtatttgatc aaagtcaaat aatcaacagc cgatagatag tcggataact
     8641 gattcgttga ttctagaaaa aagaaagacc ggcgataatc tgtataagca gtaggagaga
     8701 tgttgtgaag ggacaattga tcataagttg ttggcagcag ttgggcaatg ccattcagca
     8761 aagttgtttt gccaaatccg ttcggggcaa tcaaagcagc tacttctcct gattctacta
     8821 aaagattcaa ttgtcgaatc acacgttttt taccacgttt catgttcatg tttttgatca
     8881 gtaatgtcat agataggcct ccaatataaa atttaataac ataatagaat ggaaaacatc
     8941 taaattaaat gctaacaaaa gttattgttt gcatattata aaaaaattaa aataacagtt
     9001 ttatttaaaa ttgaagtttt ctgataccga aatcttttta ttttgctgac atactcaaac
     9061 ctttggcaca aagtttcgtg ccaaagtacg actatttctc tttttagtaa gcggatacaa
     9121 ttagagtatc aaaaggagag agggagagac aaaatggaac ggacgattcc caaaaacaac
     9181 atttcgctaa aagcgagggt acaaaaatta ggcagtactt tatcaagcat ggtcatgcca
     9241 aatattggag cactgattgc ctggggtgta ttaacggctc tatttatccc agatggttat
     9301 ttaccaaatg aggctttcgc taccatggta agcccaatgc taacttatct gattccttta
     9361 ttgatcggtt atactggcgg aaaagtaatt gctggtgacc gcggatcagt tgttggggca
     9421 attgcaacga tgggtgtgat tgtcggaaca gatattccaa tgatgctagg cgcgatgatc
     9481 atgggacctc ttggtggata tgccatcaag aaatttgatc agctattcca aaaacggatc
     9541 aaatccggtt ttgaaatgct agtcaataat ttttcagcag gtcttatcgg ttttggatta
     9601 gcgttattag gtttttctgc gattggtcca gttgtcgatg cattgactca agcaatggca
     9661 aaaggtgtag agatcatttt atctgcccat ttgattccat tgacaagtat ttttattgaa
     9721 ccagcaaaaa tcttattttt gaataatgca atcaatcatg ggattttgac accattggga
     9781 actgaacaag tagcagaggc tggaaaatct attctattct tgcttgaagc aaatcctggt
     9841 ccaggacttg gcgtattact tgcttttact ttgtttggca aaggagcagc aaaatcttca
     9901 gcaccaggag ccatgatcat tcacttttta ggcgggatcc atgaaatcta ttttccttac
     9961 gttatgatga aaccgttatt attcttatca gttataggcg gaggaatgtc aggaagtttc
    10021 attttccaat tgatggatgc tggactaaga gcgccagctt ctccagggtc gatcattgcg
    10081 attctagcga tgacaccgtt caattcttac cttcctgtta ttttgggcgt gattgttgcc
    10141 gcagctgttt cgtttggtat ttctgcagta attttgaaag cggatgcgaa agatacaaat
    10201 gaagacttcg aaaaatctgt tcaagagaca aaggcagcta agcaagcttc caaaggtgcc
    10261 caaggtattc ctcaaggagc tacattctct ggcgtacaaa aaatcatttt tgcttgtgat
    10321 gcaggaatgg gatcaagtgc gatgggcgct tcgatcttga gaaaaaaagt acaggaagca
    10381 gacttgcctc aaacggtgac caatcaagca atcagccaac tgacagatga ctcgaataca
    10441 ttgatcgtta ctcaggtcga actacaagaa agagcaaaac aaaaagcacc gagcgcgcgt
    10501 tttgtctcag tagaaaactt tttgaattca cctcgatatg atgaaatcat tcaagaatta
    10561 agcagcaagc agaaaacatc agtttctgaa attgagaatg agccagcaga agagtttctt
    10621 tcagcaaaca tcaaacaaat caaagagatt ctacttgtcc acgatgatcg ttccggatca
    10681 gcaacaatgg ggatgaccgt gctcaaagat atattagaca agaaccattt aactatcccg
    10741 ttaagaaaag tcaatatcca agatttaaaa gatgaacctg attcactgat cgtagccaaa
    10801 gaaagtttag cggaaaaagt acagatggct gcacctcaat ccattcattt atcagtcaac
    10861 agcatggtca atccgcaaaa atacgaatcg attgcacaat atttgaaaca gacagcttaa
    10921 gggaggaaaa cagcatgcaa agtttgccat tcgacatgat cgagctcaat cgatctttta
    10981 aaaccaaaga agaagcgatc cgctattgcg gtcagatgct tgtagaagca gggtgcgttg
    11041 atgaggaata tatcgatgcc atggtagata gagacaaaat gttatctgtt tatatgggga
    11101 attttatcgc gattcctcat ggaacagatg aagcaaaaaa atatgtgaaa aaatccggca
    11161 tctgtgtcgt tcaagtacca gatggtgtag attttggtac cgaagaagaa gaaaagattg
    11221 cgactgtttt gttcggtatt gcaggagtcg gagatgagca tttgcaactt gtacagcaaa
    11281 tcgcacttta ttgcagtgat atggacaatg ttgtccagct agcagacgcg ctcagcaaag
    11341 aagaagtaac ggaaaatcta gcaattgctt aaggagagag aaaaatgaga gcagtacatt
    11401 ttggtgcagg aaatatcggt cgaggattta ttggagaggt tttggcagct aatggatttg
    11461 agattacttt cgtagatgtg aatcacacga tcattgatgc tttaaatgaa agaaaagaat
    11521 acgaaatcga attagcagat gaatcaaaag aaaaaatcac tgtaaaaaat gtcaaaggaa
    11581 tcaacaatca aacaaatcca gaaaaagtaa tccaagaaat cgctgaagca gatttagtta
    11641 ctaccgccat tggcccgaat atcttacctt ttattgctga gttgattgca aaaggtatcc
    11701 aagaaagaga aaaggaagga agcacaacat ctttagatat cattgcctgt gaaaatatga
    11761 ttggcggttc agcatttcta gaaaaagaag tgtataaata tcttccggaa acatcattta
    11821 ctgacaaata tatcggcttt ccaaatgcag cagtcgatcg aatcgtccca ttacaacacc
    11881 atgaagatcc tttattcgtc caagtggaac ctttcaaaga atgggtcatt gacgatagcc
    11941 aaagaaaaaa taaaaaaatc caattaaaag gggttttata tgtagatgat ctagaaccat
    12001 atatcgaacg caaactattt agtgtcaata ctggtcatgc gactgtcgct tatacaggtg
    12061 ccttgttagg ttaccagacc attgatgaag caatgcaaga tgcgttagtt gtggcacaat
    12121 taaaatcagt tttgcaggaa acaggaagtt tgctgattgc caaatggggg tttgatgcag
    12181 agcagcatca tgcgtatatt gaaaaaatca ttcatcgctt ccaaaacaaa aatatctctg
    12241 atgcgatcac acgtgtcgct cggacaccat tacgtaagtt aggttatcaa gaaagattta
    12301 cacgtccagt gagagaacta caagaacata acttgacttg cccacatttg acggcaacaa
    12361 tggggattat ttttaattac tatgatcctg aagatgaaca aagccgtcaa ttgcatgaaa
    12421 tgaagatcca tgaaaacttg gaacaattga ttcaagaagt cacaggaatc aatgatccga
    12481 aaacaatcgg gaatatcaag caaaacgtga accgttatgc caagcaagtc gcttaaaata
    12541 ggatacgaaa aaggggaatg tgacgaaatt tttgtcacat tccccttttt cgaataacta
    12601 ggctgtttgt ccttcaagat agggcagatc caattcttta ccgtaacgtt gttctaaagc
    12661 taggcggatc aaacgagctg ccaggttctc atttcttgca aggataggtc catggaaata
    12721 agaaccgaag acatttcggt aaatgacacc ttcagattgg tcttcaccat tatttccttg
    12781 accttgtacc actttcccca aaggtcgttc accttcacct aagaaagtcc gaccattgtg
    12841 gttttcaaat ccataataag tttcattgaa ttcttcgtta tggataacga tatcaccgat
    12901 aaaccggtta ttttcctgac ttaacgtgta gtgatccagt gcactgattc cttggatctt
    12961 ttctccatta gctcccatgt agtagtgacc tagcaattgg tagccaccac aaatggctaa
    13021 catgacgcca tcattttcga tatagttgat caaagaatct ttctttttct ggatgtcttt
    13081 tgaaatgatt aattgctcaa agtcttgacc accgccaaag aaaaccaagt catatttatc
    13141 agcatcaaaa ggttcatgta tactaacgat ctccgtatgg caagaaaccc ccattttttc
    13201 tgctatatat ttcaacatca aaaggtttcc gttatcacca tatgtgttca tcaaatttcc
    13261 ataaaggtgg gcgatattga gttcataatt agccacggtt cattcctcct ttgataaatc
    13321 cttgagcagc tagttcttta cgaagctgca atacagcagt gtaagtagct aaaatataga
    13381 catgctcagt tggcaattct ttgatttttt cgattacttc tggtaattct tttgtttctg
    13441 tcaatttatt ttctggaatc ccggcaactt ttagacgtaa agtcatatct gtatgacggt
    13501 ctcctccaga aataacagct gggatatcca tatcggcaaa gttttcaaaa ttaccatccc
    13561 aaatccagct gacatcgatt ccgtctgcgt aattggcatt taataaagcc actaaagaaa
    13621 atggataagg agctagacca atcatatcga ttacttgatt gatacctaca ggatttttga
    13681 caagtactaa tgtacatttt ttcccattga tattgatgac ttcttgtcgg ccgaatactt
    13741 tctcgtcata tcctaaacct tgtttgattt tatcaggatc gacattgaag tactctgcca
    13801 ctgcagtagc tgctaaagca ttgtagacat tatacatacc gccaacttcg attccgtatt
    13861 catgaccatc gatcacgaaa tctgctgaca cattgtccat ccgtaccata tcagttaatc
    13921 gataatccaa ttcaggacgt ttgaagtcac aatttggaca gtaatatttc cctaagtttg
    13981 cataagtgat cattttataa tgcaagatgt gatgacaatt tggacataag acaccgtctg
    14041 tattgtaatg agccatttgt tcacgatctt cttcatggtc aaagccataa tattttctag
    14101 gattgactgt ttcgatagaa ttgaagattg gtgaatcacc gttactgata atcgttgcat
    14161 ttggtgactt tgcggcaccg tctacaatca ttttataggt cgtatagatt tctccatacc
    14221 gatccatttg atcacggaaa atatttgtaa ataagaacag ttctggtttc atatattctg
    14281 tgactttgct caaacttgct tcatctattt ctaggacagc aaactttttc tgtccttttt
    14341 ttggttttgc ctgcaagaaa gtcgatacaa ttccctgtac catattcgca ccagtagggt
    14401 tggttagaac ttcatcgaat tgctggcgca aaatattgac tgttaaagcg gtagtcaatg
    14461 ttttaccgtt tgttccggtg acaacgacga cttggtagtc ttttgccaaa ctatctagta
    14521 tatgtggatc gactttcagc gctagtttac ctggtaaact tgatccgcct ttaaaaaaag
    14581 tttttaatgc ccattgcgac gtttttccaa ctgcaatcgc aaaatgactg cgtattccca
    14641 ttatgtaacc tccaaaaacg atttgctgac gaataaattc aactaatcat accatatgtt
    14701 gaaaaaaagg ttaaattgct tttcttattc ttaagaaaag gttacggctg aaagtgcatg
    14761 aaatcggaca aaatcgcatg atttaacaaa aaaacagctt gaaacaaaat gattgtttca
    14821 agctgctgtg attttattat tccctcttac ctatattccc attttaaccg ataataattt
    14881 tctcgcttgg atattcatag cctagatctc gtgtttcttt tggcacgaat aacatcaatg
    14941 tatatagcat tccgatccgc ccaataaaca tcagtgtagc aatcgtgatt ttgcccacaa
    15001 cagacaagtc tccggtaatc cctaaagata aaccagttgt tccaaacgca gaagttactt
    15061 cgatgatgat cgagatcagc gtttgttctt ctgtagcaga taaaaaaacg atacaaaaga
    15121 aacacatacc aagagaaagc ataaagacaa cgactgattt tcgcacatca tcttgatcga
    15181 ttctccgtcc aaaaatattg atgttatctt cacttttgag gaaggaatat agatacaaac
    15241 cgatgattgc tacagtagtt gttcgaatcc cgccgcctac agaactagga ctgcatccga
    15301 taaacatcag taaagaaaaa atgatcagtg ttgtgatttg aaaatcaccc aaatcgtgaa
    15361 tctgcagacc agcattacga gtcgtaatcg aataaaacat cgaattgatc catttgacac
    15421 tcatattgga atcttggaat aaatggtcct tttccaatag ataaatcaag acagttcccg
    15481 aaacaaacaa gataacaaaa gctaacacag ctaatttggt aaacaaggaa aaacgaaacg
    15541 gaagcttcgc attggaacgt ttgtataata gccattcccg gcactccatc aatacaggaa
    15601 aaccaattcc tccgacaaaa atcaaaaaca tgatcaggat caagaaaaaa tagtcatgtg
    15661 caaagggctt gatggaatcc ccagtgacgt caaacccaga gttcgtgaca gccgatatcg
    15721 cctgataaaa accgtaaaaa acggcatcac gccaacgatc aaaatagcct ctgtaataaa
    15781 aataaataga aaagaatgtc ccaaaaagta attgaaacca gattaaaata gcaaaagtga
    15841 tccgaatcag tcggacgata ccagacaaac gcggttgatt catatccgtc atgatcagct
    15901 gtcgctgttt cagtgtgatc cgtcgtttag aaaaaatgac aaaagcagta gaaatcatca
    15961 tgatccccaa accaccaact tgaaataata tttccagcag tataataccg ttatcattga
    16021 acacagaatt gatatcaaaa gtcgatagtc cagtcacact cactgtactg attgccatga
    16081 acagcatgtc cacaaaagaa acatggctat ctggttcgcg aaaaatcggc atataaaata
    16141 gaacgagtga taagaccgtc atcaatatat aataaaaaac gatgatttgg atcgatgaga
    16201 aatgattggc agcaaacttc tgtcctcgcc gctgcagcct tcgaaagaat aaatcaacat
    16261 gcacagtact atcctcctga caatagtcgt ttctaccagt atacaagggg aattaggaat
    16321 actcaaagaa attatcaaac agataacaat acagctttcg attaaaatag aaatggctta
    16381 taataaggaa aatcctagaa aaaagaagga ggaggaataa caaattgatt aatgctcaac
    16441 aaatcgtaga aaaaatgaaa aatcaaaaaa taaattatga tacagttctg aaaaaaatga
    16501 tcatgcaatg ggagaaagaa gaagtaaagc ctaagatatt aatacataca tgctgtgctc
    16561 cttgcagtac gcacacattg gaattcatgt ctcaatatgc agagatcacg ttattttttt
    16621 caaattcgaa tattcatcct aaaagtgagt atttgcgaag attacatgaa caaaaacgat
    16681 ttgtagacca atttaatgag aaaactgacc atcatgttgc gctaatcgtt gatgaatacc
    16741 gtccaaatga attcaccaaa atggttttaa caaaccatct tgaaaacgaa aaagaaggtg
    16801 gtgctcgctg cagtgcatgc ttcaatatgc gattagatct tgttgcgcaa aaagcgcaag
    16861 aactaggata cgattacttt ggaagtgccc tgaccatctc gccaaagaaa aatgcagctt
    16921 tgatcaatca gatcggaatg gatatccaaa agatttacgg gacacaatac ttgccaagtg
    16981 atttcaagaa aaacaaaggc tatgaacgct ctttgcaaat gtgcaaagag tatgatattt
    17041 atcgccaatg ttattgtggt tgcgtgttcg ctgccaagca gcaaggatgc gatctgaaag
    17101 aaatcaatcg ggaagcaaaa gcttacttaa aaacatcaac cgtagttttt gaggaaaatt
    17161 aaagaaatct ttcggaaaaa cgtaagaaaa aaataaagaa tttatggtag aaataaggta
    17221 gactgtataa tttgtccgta gtaaagtaag gaaacaagaa taaatttcaa tgaaggaaga
    17281 gataagatgc gaaaagtagc acctattttg aagccaatcg ctgtattaac aatctcgacg
    17341 attgtcgcga ctactacagg tattgtgaat agttccagct accgtgcacc aagttcaagt
    17401 tctagtactg tagaagaaac aacaccaact acttcagtct cgacatctga accaacaaca
    17461 acggaaagtt tgaatgaaac cactacgaca agtacatggg acgatacaac aactagttcg
    17521 tcgtatgatg agacatatac aactgacagc tcaagcgaaa caagtaatac ggtaccagat
    17581 ggcactccat cggattcaac caatccaagt acaagtccgg atacaagtgc tagctccgaa
    17641 acgataccaa gcagtacgac tgtagatgat aacacgcaaa atgatgatgc agcacgtaca
    17701 gcacagcaaa accaagattc aggaaatacc caaacgtata accagcaagc aggtgattct
    17761 caatgacatt tttactagac cttatcaacg gggtgcataa attccttggc tatttagaca
    17821 tcagtcctaa atatcttaac cgtggatata cgattttaag tgtgattccc acgctttatt
    17881 tattgagaat tgtttatgga ctttggcaaa accaaaacta tcttcaattt ttcttatatg
    17941 gacttgcatt tctagtgctt gtttatttta ctgttttaaa tgtattttat tactttttag
    18001 ataaaaacac gaaagccgat gtcactcagc tttttgtgaa gtatttacct gatgaagcgt
    18061 tcaacatcca gacagaagaa tcgatagata atggaaattc gatcgataaa gtgaatacac
    18121 aagaggtcat ggtttcttat gacgaagatt atcaattgaa attagccgag aatatgcggt
    18181 atttgattgg aaatggtgag atcaaaacaa atgaactcgg tcgcatggat ggatttctgg
    18241 tcgatagaaa tacattgtat ccttattact atgtcaagaa aacaggagag aaagagtatg
    18301 ttctaaatat tggacgaagc tactcggaca tggaaaaagt agggaccatt cgacttgact
    18361 caacagaaga cacattggaa cctgtcggat tgttcatcgt cggcggtgat tttgtcaaag
    18421 acgggttacg ctatcatgag ccttatcgtc tgaaattgat tgcaaaaaag gcacagtcgc
    18481 aggaagaacc agtggtttat agtcgtagcc aaagaagaag acattcataa tgtgatgaaa
    18541 cgccgtgtat cagtggttgc gataaaaacg tttattcgga ataaggggga tgtgacacgt
    18601 cttttgtcac gttccttttt tatataacga gaaagataag acgaacacat cacttattct
    18661 ttcctttcat ttataaacat tgtagaataa agaaagtaca acgaagtatt gtgaaaaaac
    18721 gtaataaata acgaggaaaa tacaagtagg aggtaagctg tgaaagaatt tttgaaaaat
    18781 tgggggattt tgatccttgt attagcggct attttattag ctagagtgta cgtgtttaca
    18841 ccagttactg taaacgggca ttcgatggac cctacattaa gtgatggaca acgattgatt
    18901 tcatctaaga tctcaaacta tgaacgaatg gatattatta ctacaaagga accgggggat
    18961 gaagaacgga tgattgttaa acggatcatc ggtatgcctg gagatacagt caagatggaa
    19021 aatgaccagt tgaccattaa tggcaaaaag tatgatgaac cttatctgga tgagttcaaa
    19081 aaagaatttt cagaagataa gttacaagga gaatatgctt atagctcagg attccaagca
    19141 caagcagaaa gttcatccac ctttacgagt gactttgaat atactgtgcc aaaaggaaag
    19201 tatcttgttc taggcgataa ccgcttgatt tcaaaagaca gccgaatgtt tgggttagtc
    19261 gacaaagata tgatccaagg aaaagttgtt ttccgttatt ggcctctttc agaaattaaa
    19321 atcatgtgat gaaaaaataa aaagaagctg tgacatgttt tttgtcacag cttctttttc
    19381 cgaaaattcc ttcttatttc ttggagttgc tcactttttt tgcaatctca ttttaagctt
    19441 tttggctgat ggataagaaa agcgaagcgt ttttgttcat aaatagcttt tccccacgat
    19501 ggaaggtatg actagtagca gagacaaagc ccgttttttc caatcgatta ttcaattctt
    19561 cttggctaaa gccattatgg actttcggat gactaatttg ttcattcttg tcaaagtcta
    19621 cgatgatcaa ttgaccgcca ggagctaaaa tttcataaag ttttgtaaga atattctccg
    19681 tatcaggaat gtgtaaaagt acaagtgata gaaggatgag attagcttgg acagcaggaa
    19741 attcgacaga agcatcagcg tggatcgttc ggacattttt cagatctgct gcttggatct
    19801 tttcttctgc cattttcagc atagtttctg aagcatcggc aatgatcagt tctttgaatc
    19861 tttccgcaag cggtaaacta acaagcccag ttccgcctcc gtaatccagc aggacttttt
    19921 ggtggctgtc gcgcggtaga taagtagtta gttcttcgcg aataatcttg gctaatgctt
    19981 gacggtcagg ggaatcataa tggttagcga tggaatcaaa aatatttttt gtcatcaaat
    20041 agctccttac gtaaaacttt gaaattttaa cttattatag cacgtgaaaa gaaagaaagc
    20101 atgctatact caattcaaaa agggagaatc tagaaagaat atgccgatat cgtttgtaaa
    20161 agatagagag gaaaaaggaa agtgtgtccg agaaatatta ctagacttgc ctgagtggtt
    20221 tgggcttcct gaatcaacgg aaaaatatat cgaggaatca agtaaactac ctttatggtg
    20281 tgaaaaaaga aaagaagaat atttaggctt tatcactctt tcacagacaa gtgaagatac
    20341 tgccgaaatc tattctattg tatgggaata aaaaaagact atcatcacca aggattagga
    20401 acaaaacttt ttaaagaagc agaaggttac gcagcaaaac attacaaata tttgcaagtg
    20461 aagactgtag acgaggggca ctactctata tatgatcaaa ctatttgttt ctacgaatca
    20521 ttaggatttt caaggttgga agtttttcca aatttatggg atgaatggaa cccatgttta
    20581 gttcttgtca aaaaattgga acaaaagtga gcaatagatt taatactaat ctgtgcttga
    20641 tgaaaattct cactgtaaaa aatgctgaaa atcaagtata ataccatagg aacaaactgg
    20701 aaggtgaaaa agagcatggc acaactattt tttaagtatg gcgcaatgaa tagcggcaag
    20761 acgatcgaga tcctaaaagt cgcacataat tatgaagaac aaaataagcc agttgtctta
    20821 atgacaagcg gtattgatac aagagatggt ttaggcgttg tttcaagccg aatcggactg
    20881 aaacgtaatg cgatgccgat atttgaagaa acagacgtat ttgacgtaat tcagaaaatg
    20941 gaaagcaaac cctattgtgt gctgatcgat gaagcgcaat tcttaaaaaa gaaacatgtc
    21001 attgctttta cgagaattgt ggacgaattg gatattccgg ttatggcatt tggtttgaaa
    21061 aatgattttc gaaacgagtt atttgaagga tcaaaatatt tattgcttta tgcagacaaa
    21121 atcgaagaaa tgaaaacaat ctgttggttc tgccacaaaa aagcaatcat gaatttacat
    21181 tatattgatg gaaaccctgt ttatgaaggc gatcaagtgc aaattggcgg aaatgaagct
    21241 tattaccccg tttgtcgaaa acattacttt cacccatcaa tgattacaga aggagaataa
    21301 gcatgtacga tcaattacaa gcaattgaag accgttatga agagcttgga gaacttttaa
    21361 gtgacccaga agtgatttct gatacaaagc gttttatgca gctttcaaaa gaagaagcaa
    21421 acacgcgcga aacagtcgaa gtttatcgtc gttataaaga agtagtacaa ggaatcaccg
    21481 atacagaaga actgcttggt gaaaaactgg atgatgaaat ggctgaacta gcaaaagaag
    21541 aattatctga attaaaaaaa gaaaaagaag tcttagaaga aaaaatcaag atcttgcttt
    21601 taccaaaaga tccaaatgac gataaaaata tcatcatgga aatccgtgga gcagcaggcg
    21661 gagacgaagc agcattattc gctggcgatc ttttcaatat gtaccaaaag tatgctgaag
    21721 cacaaggctg gaaaacagaa gtgatggaag ctaacgttac cggaatcggt ggatataaag
    21781 aagtgatcat gatgatcacc ggagacaacg tatattcgaa attaaaatat gaaagtggtg
    21841 cgcaccgcgt ccagcgtgtt ccttcaactg aatcgcaagg gcgtatccat acttccaccg
    21901 caacagttgt cgtgatgcca gaagctgaag aagtagagat cgatattgaa gacaaagata
    21961 tccgtacaga tatctatcat gcttcaggtg ctggcggaca gcacgtcaat aaaacagcat
    22021 ctgctgtacg tttgactcac ttaccaacag ggatcgttgt tgctatgcaa gatgaacgtt
    22081 ctcaaatcaa gaaccgtgaa aaagcaatga aaatcttgcg tgctcgtgtg tacgataaga
    22141 tccagcaaga agcacaaagc gagtatgatg ccaatcgaaa atcagcagtc ggaactgggg
    22201 atcgttcaga acggatccgg acgtataatt tcccgcaaaa tcgtgtgact gatcatcgta
    22261 tcggattaac tatccaaaaa ttagatcaga ttttagcagg gaaattagat gagatcgttg
    22321 atgcattgat catgtatgat caaacatcaa aactggaagc tatgcaaaat gggtaaaatg
    22381 acctatcgag aagtcctgtc acgggcttct tcttttttag aggatcatgg gaaagaagca
    22441 tatagtatcc aatttttatt tttggaacga aaacaatgga aaaaacttga ttggctcctt
    22501 catatgaacg aagagatttc agaagaagaa cagcggttga tcgaaaccga tctgcgactt
    22561 ctgcttgcag atcatccacc tcagtatttg ctaggatatg cagattttta tgatcatcgg
    22621 ttaaaggtaa ctgaagcaac actgatccct cgaccagaaa cagaggagtt agtcgaatgg
    22681 tgtttagacg agacgcctgg tgtaccgtta gaagtgatag atattgggac agggactgga
    22741 gcaattgcta ttagtttgaa agcagcaaga aaaaattggc atatatctgc tgtggatctt
    22801 tcagaagaag cattagaagt agcgaaagaa aatgctcaga aagaaggaac gaaaatcagt
    22861 ttttatcatg gagatacatt aggaccagta atggatcagc aatttgatgt gattatttct
    22921 aatccgccgt acatcagtcg gaatgaatgg gaactgatgg atgaaagtgt ccggtcattc
    22981 gaacctaaaa tggccctttt tgctgagaac gatggattag ctatttatga aaaaatagca
    23041 gttgaagcac caagcgtact aaaatcaaat ggaaagattt ttttagaaat cggatttcgc
    23101 caaggtgaag cagtaaaaaa aatatttcag caagcatttc cagataaaaa agtggcgata
    23161 aaaaaggatc tttttggcaa tgaacgaatg atccaagttt atggttgaag ttgtggatga
    23221 aaaatttttt atttgttttt ttattataaa aaaacactta tttgacaagg atgtaaacga
    23281 ctttttggtt tatcaacaag tttatccact attgacaaat agattatcca caaacagtgg
    23341 aaaacttgag gataaactca gacaaaataa gaatgtatga aaaaggggtt gtggataaac
    23401 tgtggaaacg aaacgatatg atgaaacaga attgcaagag gcagccgaag cactgaaaga
    23461 aggagaactt gttgcttttc ctaccgaaac agtatacgga ttgggcgcca acgctctttt
    23521 accaaacgct gtcaagaaag tgttttctgt aaaagggaga ccgcaagata atccattaat
    23581 cgtccatgtc gcctcttttg aacaagtaaa agagtatgtg gacaactttc atccggcagc
    23641 agagaagatt gtgaagaact tctggcctgg accattaact ttgatattta agatcaaaaa
    23701 agacactttg ccctcagttg tgacgggagg attatccaca gctgcatttc gtataccgga
    23761 taacaaaaaa acactagaag taatcgaact ttccggtgtt ccgcttgtcg gtccaagtgc
    23821 aaatacgtcg ggaaaaccaa gtccaacgac tgcagatcat gtatatcatg atctacaagg
    23881 aaaaattacc gggattattg atgatggagc tacgcgaatt ggtgtagaat ccactgttct
    23941 tgatttaagt gatccaacag ccatgcctat gattttacga ccaggtgcag taacgaaaga
    24001 acaaatcgaa gcagtcatcg aatctcctgt tgctattgat cagcatcttg taaaagaaaa
    24061 tgaaacacca aaagcccctg ggatgaaata caaacactac tcacccgata cacgtgttct
    24121 gatggtcaga gatggcgatt ggtcaactgc agttcaatgg gcaaaaaata agaagatccg
    24181 tgcaggtgtc atagcaagcc ccgaaattgc ggatcaagtg cggacagata ctgcagctgt
    24241 ttatatgtat aacgacaact cagtagaagc ggcggctaaa ggattgtttg caggtctaag
    24301 aggattagat gaaccaacac ttggattaga tttgattttt gtacaagtgt atccagaaac
    24361 tggattgggg aatgcttaca tgaatcgctt gaaaaaagca gcaggacaaa attattttga
    24421 aaaatgatca attggttatt gtcgcattct tttagtatga tacaatgcta tcaagtaaat
    24481 cttctatcag tgaacgaaaa actaagcgta actaatcgta atagaagaga tacgaatatt
    24541 tggcaatttg tttttgatgg gaaaaataaa attgtaggaa aataaaattg taggaaaata
    24601 aaaaggagct gttgtttgtg gtagattaca aaacgtttga ccctgatctt tgggctgcga
    24661 tcgccaaaga agaagaaaga caagaacata atttggagct gattgcctct gaaaactttg
    24721 tctccgaagc agtgatggct gcacaaggaa gtatcttgac aaataaatac gcagaaggtt
    24781 atccagggca tcgctattat ggtgggtgtg aatttgtcga tatcgttgaa aatttagcga
    24841 ttgaccgtgc aaaagaatta ttcggagcga aatttgcgaa cgtccagcct cactccggct
    24901 ctcaagccaa cacggctgct tatctcgctt tggtagaacc aggggacacg atcttaggaa
    24961 tggatctttc cgcaggcgga catctaacac atggatcacc agtcaacttc agtggcaaaa
    25021 cctatcactt tgttgcttac ggtgttgatc cgacaacaga agttattgat tataatgtgg
    25081 tacgtatttt agctagaaaa catcaaccaa aattgatcgt agcaggtgcc agtgcttacg
    25141 gacgaacgat cgactttgcg aaattcagag aaatcgcaga tgaagtaggc gcaaaactga
    25201 tggtagatat ggcacatatc gcaggattag tagcagcagg acttcatcct aatccagttc
    25261 cttatgcaga tatcacaacg actaccactc ataaaacatt gcgtggccct cgaggcggca
    25321 tgattttgac aaacgatgaa gcattagcaa aaaaaataaa tagtgcggta tttcctggta
    25381 ttcaaggcgg ccctctagag catgtgattg cgggtaaagc agtagcattc aaagaagcat
    25441 tagatcctgc atttaaagaa tatagtgaac aaatcatcgc caatgcaaaa gcaatggtca
    25501 aagttttcaa tcaagcaatt ggaaccagag tcatttcagg ggcgacagat aatcatctga
    25561 tgttgatcga tgttcgcgag ttaggtataa atggaaaaga agcagagagc attttggaca
    25621 gtgtgaatat cactgtaaat aaaaactcga ttccttttga aacactaagt ccattcaaaa
    25681 caagcggtat tcgaatcggc acgccagcaa tcactactcg cgggttcaaa gaagaagatg
    25741 ctgtaaaagt ggctgaacta gttgtcaaag cattacaagc aaaagatgat aacgctcaac
    25801 tagacgaagt caaaacaggt gtacgcgaat taactgaaaa atttccgttg cataaaaaat
    25861 aaaaatttaa attccattag taaacagaaa agttcaatgt tgggcttttc tgtttatttt
    25921 ttgggaaaac ggattctttg acaatatcgg tgatgctctc tagtatatga ggtgtaattt
    25981 tagtcttcac actaaatctc agggaggaaa agtcatgact gatcgtatct cgttagaaaa
    26041 ggaagcagta gcattcagtg atgcaaatgc gcctcatccg cgtatctatg aactagcacc
    26101 ggaagaagga agagttttac tagaagaagt acaagaatca cctgtgaaca aatattccgt
    26161 cgatattgaa gacttgatgg tggatactgg cgaatgggga aaactcaata ttcgtctcgt
    26221 acgaccagaa gggaatatgg ataaattacc agttattttt tatattcatg gagcaggctg
    26281 ggtttttggc agtgctcaaa cccatgataa attgatcaga gaacttgcgg ttcgtacgaa
    26341 ttccgtcgtt gtctttccag aatatagtcg atcaccagaa gcaaaatatc caacagcaat
    26401 tgaacaaaat tatgctgtgt tgcaacagct gaataaatta gccaagtcaa aaaatctgga
    26461 tctcgagaat ctaatcgttg caggagattc agtaggtggt aacatggcta cagtcatgac
    26521 gatcttaaca aaagaacgaa agggcctccc aatatccaag caattgctgt attatccagt
    26581 cacaaacgct gattttgata cagattccta tcatgaattc ggagaaaact attttttgac
    26641 aagagaagga atgatgtggt tttgggacca atatacgacg aatgaagaag agcgtgctga
    26701 aatcacagct tcgcctttga gagcaaatat aaatgaacta aatgatcttc cttcagcaat
    26761 gattttaacg ggagaagcgg atgtattaag agatgaaggt gaggcttatg cgcgcaaact
    26821 tcgtgatgca ggggttcctg ttacacaagt acgcttccaa ggaatcatcc atgattttgt
    26881 catggtaaat gctatggatc agacaaaggc aacgcgtgca gcaatggcat tatctacttc
    26941 ttggatcaag aaaaaagaat aagctttgct tgtccgcagg aaaacttctt ttcaaaaacg
    27001 gattttctta ggaaaaatgg cgagtttgac tagtcaatca cttgattttg ttttacaatt
    27061 aaaaagaaac gacagaacaa aggagagctt aaaaaatggg caaatttcaa gtgatcgatc
    27121 atccattgat tcaacacaaa ctaactatta ttcgggacaa aaactgcgga acaaaggttt
    27181 ttcgtgaagt agtcgatgaa atcgctatgt tgatggcata tgaagtatca cgggatatgc
    27241 cattggaaga catcgtgatc gaaacaccga tcactgaaac aacacaaaaa acgttgtctg
    27301 gtaaaaaagt agcgattatt ccaatcttgc gcgcaggtat tggaatggtt gacggtatct
    27361 tggaattgat tccagctgct aaagtgggac atatcggtct ttatcgagat gaagaaacat
    27421 tggaaccaca tgaatatttc gtcaaacttc cagaagacat tgatgcacgt cagttgtttg
    27481 tagtagatcc aatgttggca acaggcggat cagcaatcat ggcaatcgat gcattgaaag
    27541 aacgtggcgc gtcgaacatt aaatttgttt gtttggtagc agcacctgag ggagtaaaag
    27601 ctttgcaaga agcgcatcct gatattgaca tctatacagc ttctctagat gaaaaattga
    27661 atgaacatgg ttatatcgtt cctggactag gggatgcggg tgaccgtttg tttggtacaa
    27721 aataagtaaa tacgaaaaag ggtgtgacac aacttttgtc acatcctttt ttggtcattt
    27781 ttaacgtttg atgcagaaca gctttttcac agctttcgtt tgcatataaa aaaaccatcc
    27841 caaaaatgca gagatggtca aattcaaatc ttttttagtt ctttgtagtt aacgattact
    27901 acacccttac tttgcgtcat caagttcgca ttttctgcag tttcatcaat ctcaacaaca
    27961 gcggaatttt tcaattgctt tgaaatcgtt ccagtttgcg acttgccgtg aattgtgaac
    28021 attacgtgag tattgatctt gaatttttca ccaatttccg atggtacgat agttgtatca
    28081 aattttccaa aagttgccat tttcataaaa cctgcctttc acatcaatta taccacgttt
    28141 tatgaaaaat atcatgaaat taaactaatt ttccatcgga ctctttacaa tcagccaaat
    28201 agagtatata atttcttttg gataaggcca gcttagctca gttggtagag caacgcactc
    28261 gtaacgcgta ggtcgtaggt tcgatcccta tagctggcat taaaagagaa gacagatata
    28321 tcaaggattt ttaatccatt gatacatctg tctttttgtt taaaggaaat aaagtatgct
    28381 ttcttagcaa tagagcatgt attatttcta taaaaattta ggagaataaa acgactagca
    28441 atatagctgt gaaatcaaat atcacatgaa ctgcccaact tgtaatgatc gagttgcttt
    28501 taatggcagc ttggttgaat aacaatcttg ccaccccttg tatcaaaagg atgtgtgcta
    28561 cagtgtgtaa aatatttcca ttatcataag tactgtaatg gataaatcca aagatgattg
    28621 ctgacaagaa actagcgaca ggaatgctga acttactaga aaaaatagcg agaaaataca
    28681 ctgagaacaa ttcttctcca agtatcatga taggcaaaat aataaataat aacgggctga
    28741 aagcctcatt tattggattg cctgttaaac cccatttcaa aatttgttgc aaaaataccg
    28801 agactgcggc actgattact acattgataa aaaaatattt ggctatattt ttgacaggag
    28861 cgacaggttt cgagaacatt tttttcgtat cttttttacc aaaaacaagt gtaaatgcta
    28921 taaatccagt gatagttaaa gaaaaaatgc taataatgga tggcgcaaat agcaacccta
    28981 acaaaccgag tacaacgaca atagtgccca atatcccttt tttaagttct attttttttc
    29041 ctaaaatgtt attcatgata ataactccta ctattcagtt tttgaagata gagtcagtat
    29101 acatgaagag ttaaaatagg caatgttttt ttatggatat ttgtattgct aaaaatttat
    29161 cttatatatt cattttgatt atactacttt tatttgtaaa aattttctag caaaaccaag
    29221 tgcttgcaac tgattaagcg tacaattttt tagatgttcc gagcgtactt caatcatttc
    29281 aaaggagttg ctacttaaat cgatattttt taaatcagta tcaaaaaagc tagcattttc
    29341 taaatcatta gtagaaaaca ttattttagt gagggaacag tctacaaaat ctgcgcttga
    29401 taagacagag tgctcaaagg tactttcttt tatgtttgaa ccagataaat ttacatagtc
    29461 cattttacaa ttctcgaata acgtgttccc aatataactt tccatcaatt tagtacctgt
    29521 taatttacaa ttgacaaatt tcacgcgatg caacatgctt tttgtcaata tacagcctga
    29581 aaaatcacag ttttcgaata tacagtcggt aatctccatt ctttgaaaat cattggaagc
    29641 aaaaaatgca tacttgaaaa tagatttgta aataattgga tggcagatta tttggttttc
    29701 gatgacctct ttttgtgaaa ggatattata cagttcaaaa tcttcgtttt catagatatg
    29761 aaaaaaggat cctttattca agcttgcttc attaacttta ggaaaaatca tatttaatta
    29821 cctccatttt cataaagtca tattaacaaa agctaaacca acttacatta gcagaaaatg
    29881 agataggttt tcgccttttt cgataacgat agtgtcgcta tctcttgaaa cgttcttaga
    29941 attgccctga gagacacaaa ttaggcgtaa ttctaataaa aatcttagaa taaaaatttt
    30001 tactgattta atttagaatg tttttaaaat aaaaatcgtt gccataacag tgtattgaaa
    30061 ggttttaaca atccagtaat gatttaaaaa aatctagatg tagcgtatac tttgtttata
    30121 gaatgtagtg ggaagaaata tatttttggg aggaaatcgt gatgtctaaa agtgaagaaa
    30181 tagaaaacat ggtactaaaa ttatatgatc tcgttttaaa tccagagatc aaagaaaagg
    30241 aacgtatact tttgatagac gcaaagacag gcttagaaaa aggtcagtat tatcctaaag
    30301 tcataaataa tttggaacgt agtttacgtc cgttagcaat caggggagaa ttgtctaaac
    30361 cagtatcacc gttttatatg gagatatcaa caataggaaa atttgaaaaa gaattaggtc
    30421 gaggaatggc ttcggcaccg attacttttg ggcatttata aaaacgagtt attcatctca
    30481 atgaattaaa aagtatagtc aatctcataa tgcaggctag gttcatttat ttgaacagag
    30541 tctgtatttt tttattctaa ataaagaaac agatgtttct ttatattgta ttttatcctt
    30601 taattaacac aaaaacagct agtataaact aataatgtta attaaaagtg tattttatga
    30661 aactatcgtt atttattaat gttgcatttt tgtaagttaa aaattggaaa tatagattga
    30721 aaaaatgaaa gcaataagta aaataaaatg aatatatgcg aaaaaataat ttacattagg
    30781 aaaaaccttg taaaaacagt tttttggcat atttattttt tataaatatt tgtaaaataa
    30841 ctatcaattt tttagttaaa agattggaaa tgagtggggg aggaattgaa gaaaggattt
    30901 ttgagagatg atatagtagg ttttattttt agttaaaata ggggagatag aaaaaatgaa
    30961 aaagtgtaga aatattattt tatcaatgtt atttattatt actaatataa catcactgat
    31021 tcctgttcat gtctatgcgg atgcaggcag agatatcagc agtaatgtca cttcgttgac
    31081 cgtggaccca acgaatatta cagatggagg aaacataaaa gtaaaattct cgtttgacga
    31141 aaaaaaacaa aatatccaac caggtgatta tctttggatc aattggccaa gtgaaggaaa
    31201 tattcgcggt gaaggattcc aaaaagaaat cccattaatg attgaaaata aaaatgttgg
    31261 aactttaacg gtcagaaaag attctgcgca ggttgtgttt aatgaaaata ttaaaaacct
    31321 agactcagta gaaggttggg ggcaatttga gatacaggcc agaaacgtaa ccgataccgg
    31381 tgaagaaaat acgggaccat tcaccgtgac gagcggtgat aaaacagcta ctgtaaatgt
    31441 gacaaagcca gcttctggtt cttcttctag cgttttttat tataaaacag gagacatgct
    31501 acctgaagat actaagcaca ttcgatggtt tttgaatatt aacaataacg gcacttatgt
    31561 tgaacaacca gttaaaatca gtgatgaaat ccaaagtgga caaagattag atccaagtac
    31621 gtttgaaatt aatcaaatac acttaggtga acagaaggtt tatagaggag aagagggaat
    31681 ccaacaattt ttacaagatt tccccagcgc tacatttaat ttttcagtaa ctgataatta
    31741 cattgaaata actataccaa aaaactttgt caatcttagg aaaataatgg tgagttataa
    31801 gacgatcata gaaaacccag aacaaataaa ttttgagaat cattcggaag cttggtttaa
    31861 agaatttaat aaacctgctg ttgatggaga aagtttcaat catacagtta aaaatatttc
    31921 agcttctggt ggagtcaacg gtacagttag aggcgaattg aagattttca aatatatcaa
    31981 tgacacagag attggtatcc caaatgtgac tttcgaatta agacgagcag atgaacagcc
    32041 tattcaagga cagtctagta tcctgttgac ttcgaatgaa cagggagaag ctagtatcaa
    32101 aggcctgcaa gtaggagact atgttgtaaa agaaaaagaa gcacccaatt ggattgattt
    32161 tgatccacta agttcaaatg aactcaaatt ctccataaac gaaaatgata cagagggagt
    32221 cagtttacct atttataaca agaaaaaggt tacgaacatc acagcaacta aaatttggaa
    32281 cggtggaacc actccaagac cgagtattta ttttaaatta ttcagagcaa gtacaaacaa
    32341 ttgggaacca gttcctgatg cagaaacaaa acgattagac aatggaataa cttcagtgac
    32401 ttgggaagat atccaacagt atgatgatag tggaaacgaa tatacgttta aagtgcaaga
    32461 agtagatcaa aacggaaatg attatgtccc ttcaggttat caaaaaattg aaaacgggct
    32521 agtcgttaca aatgagaata aagaagtcat ttcagtctca ggacaaaaaa catgggaaga
    32581 caatgagaac caagacggca aacgaccgac gacgatcaca gtaaatctat tagccgacgg
    32641 tcaaccgatc caacacaaag aagtaagtga aaaagatgat tggcgctatc gtttcacaaa
    32701 tctgccaaaa tacagagacg ggcaagaaat catctatact gtaacggaag acaacgtgcc
    32761 cgaatacagt acgacgattg acgggtataa catcaaaaac agttatacac cggaaaaaac
    32821 aaatatatct ataccaggta gccatataac accgtggaaa aattttccag agaagacaga
    32881 gagagagaaa attagaaatc tattctctca aaaattgcaa atatttgctc atacaggaga
    32941 atccagcgaa caaagaagca attatattag taagagagct gtacctaaac agataacaaa
    33001 taaatcaaaa agcattcttc ctaaaacaag tagtaaaaaa tctagttttg ctgtaatcat
    33061 tggtttattg atagtaacta tctgtgctgg gatattctta caaaaatata aaaaagttta
    33121 gttagaatat aatatttttt gttaaatatg actgaaatca aaagaataaa attccgattt
    33181 ataatcgaaa aaagaagcat ttttatatat gcttcttttt tcgtagaatc aataaatatg
    33241 gatttggcta actatggaag aatactcgta ttagttttca gcgttcatac tagttgttac
    33301 attgtttgat tggccaaaag aatttctgat atattttgtg ctaaataaag aaaaagacgc
    33361 ttgaattggg aaataagaaa actactctat cattacaaat tttcataaac tggcagatgt
    33421 tctaagtaaa caagtatctt ttcaaagtcg gaaaaatcca taatcaacgt agtggtattg
    33481 atatttggat gaaagcctac ggtatcttga cgatcaatag cagcatcgat cacgacttgg
    33541 atttttttct ctttgtcgtg catcaatgct aatggagtca ctgtacctgc tggaacgcct
    33601 aggaggtcag gaagctgttc ttcagaaaca aaagataagc gcttttcttt taattgttca
    33661 gctagttgtt ttaaatcggc gtgcttttca tcaggcaaga tgacaagata gatttgtttt
    33721 ccttttcttg atttcaataa tagatttttt acttgcggac caggtaggga aaaagggaca
    33781 tttttgactg acgtaatcgc aggatgatcg actttttgaa aagagatacc cagttttttt
    33841 agtagttcat aagcttcttg ttcggttgca cgactcatcg cgcatcaccc tccttttttt
    33901 attagtgtat cataaaaaag agttgacaaa tgaaaaataa tcatgtaaat tacacgtaaa
    33961 gttaaaacaa atgacatcga aaagaagagt agccgttagg tgtttttaga gagtctctgg
    34021 ttggtggaaa gaggcagcag ttaaccgtga aacacatctt ggagtcgatt ttctgaagcg
    34081 tatagtagga aaatccgggg agcccgttac agctgcggtc gttgctagat gaccagataa
    34141 ggctggcttt tccgtgagga gtcggtaaaa aaaggtggaa ccacgtgatt aacgtccttt
    34201 gatcttcgga tcaaaggact ttttgttttc atctgaacgg caacgactgt gtgaggtttt
    34261 ttctgcaagg aaaaaataaa cagaggtgga accacgtata atacgtcctc ttggcatttg
    34321 ccaagaggat ttttttattt taaaggagga aaagaagatg gagtatttga ttgaagttat
    34381 gccgcaatta ttagtaggag cactcacaac attgaaatta tttttattta ccttgattgg
    34441 ctcattacca ttagggattg ttttagcatt aggattgtct ttgcatttta agccgctacg
    34501 ctacttgctt caaacatatg tatgggttat gcggggaaca ccgctgttgt tgcaactgat
    34561 ttttattttt tatgggttgc cgacgattgg gatcgttttt gatcgtttcg aagcagcact
    34621 cattgccttt atcttaaact atgcagctta ttttgcagag atctttcgtg gagggataca
    34681 atcgattcct caaggtcaat atgaagcagc gcaagttttg caattaagtc cctttcaaac
    34741 ggtgacgaaa atcgtcatcc cacaagtgac aaagattgtt ttaccttctg tagggaatga
    34801 agttatcaac ttggtcaaag attcttcctt gatctatata ttaggtttag gtgacgtaat
    34861 gcgtgctggg aaaatcgcga tggaacgcga cgcgacttta ctgcctttgc taggcgtggc
    34921 aatcatctat cttacattga ctggtattct aacaatcgta atgaaacaag ttgaaggaaa
    34981 attaaattat taccgctaac tgataggagg aagaaatatg attgaattga agaaagtgaa
    35041 caaagcattt ggtgaaaaaa aaataatcaa agatttgaat ctaatcattc cagatgggaa
    35101 aacattagct atcgtaggtc cttccggtgg cggtaaaacc acgcttttac gtatcttggc
    35161 tggcttagaa agttctgaca gcggagaatt ttatttagac ggtgtaccgt tcaacccatt
    35221 cgagcttaaa atgaaagaac aagttgtagg agtcgtattc caagacttcc agctatttcc
    35281 acatctgaat atttttgaaa acattacctt agcaccaaaa atggtattga aacagacaaa
    35341 agaagaatac accaaaaaag cagaagaatt agcggcttca ttagggatta gtgatttatt
    35401 gaatatctat ccttatcagc tttctggcgg acaaaaacag cgggtagcga ttgccagagc
    35461 attggctatg aatccacggg tattggctta tgatgaacca acaagtgctt tagatcctga
    35521 attacgccag caagtcgagc gtttgatcct atcattgaaa gaagatgggg taacccaaat
    35581 tattgtcacc catgatttag tatttgcaga aaatgtcggt gatcaattac taaaagtttc
    35641 cccaattcat taagaaagga gaagatagag atgaaaaaaa ccagttttct attcgctttg
    35701 atcgcaggtc ttcttctttt tgcaggctgt tcgaacaaaa aaacttcagc agataattgg
    35761 gcgactatca acgaagagaa aaaaatcgtt ataggtttag acgatacatt tgttcctatg
    35821 ggatttagag ataaagacgg gaagctcaca ggatttgata ttgatttagc caaagcagtc
    35881 tttgaacaat atgggattac ggtagacttt cagcccattg actggtcaat gaaggaattt
    35941 gaactgaaca atggaacgat cgatttgata tggaacggct attccaaaac gccggcaaga
    36001 gaaaagaaag tgcaatttac aaaaccttat atggaaaacg atcaagtgct gatcacacct
    36061 aaagaatcag gaatcacaaa ctttggacaa atgaataata agagattagg tgcgcaaaat
    36121 gggtcatctg gttatgatgt atttacgaaa caaccagaag tattaaaaga gatagtcaaa
    36181 aaccaagatg ctgttttgta tgatagtttt aatgaagcat tgatcgatct gaaaagcaga
    36241 cgggtcgatg gtctgctgat ggataaagta tacgcagact attatttgaa acaaagaaac
    36301 gaactagaag acttcaacat aacaaaaggt tcttatgaga gtgaggattt tgcagttggt
    36361 gcacgcaagt cagatagtac attagtgaaa aaaatcaatg aagccctaga tcaactagag
    36421 aaaacaggtg aattccaaaa aatatccgat aaatggtttg gcgaagatgt tacgccgaga
    36481 aattaacaaa taggggactg tgacaaaaga cgtgtcacag tccctttttc cgaataaaca
    36541 gtgttctcct gccaactcct tgtgactcgg agctgaatgg caggctcaca acctcttggt
    36601 aaacggtgtt caaaagccag tttttcggta ttaagccaaa tttatgcaaa aatgtagaaa
    36661 gccattttca caaaattttt cttactactc gaaactaagc gactttttca caacctcctt
    36721 tacataattt tattttgttg tggaaggggt ttcgtgttat tctaaaagag gaacgattga
    36781 aggttatagg aaaggaagag atgaatggaa cacaaagaac ttacctcatt tccaaaagat
    36841 tttctgtggg gctctgcttc tgcagcgtat caagtagaag gagcttggca agaagatggg
    36901 aaaggagaat cagtctggga tcgatttgta cggatacctg gaaagacatt taaaggaacg
    36961 aacggtgatt tggcagtaga ccactatcat cgttataaag aagatattgc tttaatgaaa
    37021 gaacaaggtc tgaaagctta ccgcttctct gtagcttgga cacggatttt tcctaatgga
    37081 cgaggagaag tcaatcaagc tgggctcgct ttttatgaga gattgatcga cgaattgatt
    37141 gaaaacgata ttgaacctat gttgacttta tatcattggg atcttccaca agcattgcaa
    37201 gatgaatata atggttggga atcacgtcag atcattgaag atttcactca ttatgcagaa
    37261 acattatttg aggcattccg cgggaaagtc cattactgga ttagtttgaa tgaacaaaat
    37321 atcttcactt cattaggtta tttgttagca gcacatccgc caggtgtgac agacccgaaa
    37381 cgaatgtatg aagttaacca tattgctaat ttagcaaatg catctgtcat caataaattt
    37441 cacgagttag aaatgcctgg aaaaatcgga ccaagctttg cgtatacgcc aaattatcca
    37501 attgacagta atcctgaaaa tgttctggct gctgaaaatg cagaagacct gatggccaat
    37561 tattggatgg atgtctatat gtggggaaaa tatccgattg ctgccatgag gttcttagaa
    37621 gaaaaaggat gggcaccaac gattgaagct ggcgatgcag aattgctaga atctgctaaa
    37681 ccagattttc ttgggatcaa ttactatcaa acggcgacga atgcattcaa tccattaaac
    37741 ggagttggtg ctggaaaaat gaatacgaca ggaaaaaaag gttcatcaga agaaactgga
    37801 gttcctggaa tgtataaaaa agttgagaat ccatttgtcg aacgaacaaa ttgggactgg
    37861 gagattgacc cagaaggatt aaggatcgga ctgcgtcgta tcacaagccg ttaccgcatg
    37921 ccggtactga ttacggaaaa tggcttagga gagtatgaca agttgacaga agataaacaa
    37981 atccatgacg actatcgaat caattactta caaagccacg tcaaagccat caaagaagca
    38041 atttcggatg gagcagaagt cctaggctat tgcacctggt cttatacaga tttgcttagc
    38101 tggctgaacg gttatcaaaa gcgatatgga tttgtatatg ttgattaaga cgaaacacaa
    38161 gaaggatctt tggaacgata taagaaagac agttattact ggtaccaaaa actgattgaa
    38221 gaaaatggag agaaccttta gttcacatac ttaaaacaag aaggtacggc tggatatggc
    38281 cgtgcctttt cttgataaaa tcatttagaa attaagaaaa actgtaaaat aagctgaaaa
    38341 atgaaagaaa gcgtcagttc tttcaaacaa aactttagtt cccttacttt tttttgtata
    38401 atgaaacttg atgaaatgag ggataataaa tggcacaaaa gaaacggcct actaaaaaaa
    38461 ggaaaaagac gaaaaaaacg aaaaagcaaa aacagcagca ggaacaaatg ctgacgattg
    38521 gtgttgggtt tgccttgatt ttatttgctg tcttcggctt tttaaaatta ggttttttag
    38581 gaatattgat tgcaaacggc tttcggatca ttgcaggaaa tacttaccaa atcctctgtc
    38641 ttttattagc cgtattagga ttttggattg tcattaagaa tacagagttt tcaattggta
    38701 aaaaccgacg ctggtttggc ggcatactct tttatttcgg tatcttatta ttgttacatg
    38761 cccatttatt tggcaaactc catactgggg aaccaaatat catggggacg acatgggatt
    38821 tgttagccag tgatataaaa caaagtcaag tagacaataa cgttggtggt gggatgattg
    38881 gagcaatcct ttatcatttt acttacttct tgattgcaca gcctggaagt tatttagcag
    38941 cagtgctatt actggcagga ggagcattcc tgttcagtaa tctggaaggc tatcagctgt
    39001 taaatggtat ccaatcaatt ggagaacgag tacaggaatt attggaaggt gatcctgcca
    39061 agcaagctcg aaagcaagcg gcaaaggaag agagaatgaa acagcgcgca gaagcgaaag
    39121 aagcaaggcg cttagctgca aaagaagctg cagaaaaaga agctgcagaa tatgagaaga
    39181 aaaaagcggt gagccagcaa agggaaaata ctgctgatga atggcaggaa ccagaaaata
    39241 cagagccgga gcagctaagt tttgttccaa tcgacagttt tcaagaaaat atccatcctg
    39301 cgaatctaga gaaaccagta ccagatacac caaaacagac caatacagca gaaggttttt
    39361 cagatgagtt gccagaggat gacggtacct cattagaatt tgagattgaa gcagaacagg
    39421 aaaatcaaga ctatgaatta ccgacagttg atttgcttga ttctatacca actgtggatc
    39481 aaagtgatga gtataaaaaa attgaaaaaa atatcggcgt attagaacaa actttccaaa
    39541 gtttcggtgt cgatgccaag gtcgtcaaag ccagcttagg accatccgtc acaaaattcg
    39601 aagtccagcc ggctgtaggg gtaaaagtaa gtaaaattgt gaacttaaca gatgatatcg
    39661 cgcttgcttt agcggcgaaa gatgtccgga tggaagcacc gattccaggt aaatcactga
    39721 ttggtattga agtaccaaat ggaaagatca gtatggtttc ctttagagaa atcatcgaag
    39781 cacaaccaaa ccacccagat aaattactag aagtaccttt aggacgtgat gtttctggac
    39841 gggtacaaac ggctgatctt tcgaagatgc ctcacttact cgtggctggt tcgactggta
    39901 gtgggaagtc agtagcaatt aatgggatta tcacaagtat attaatgcgt gctaagcccc
    39961 atgaagtaaa actgatgatg atcgatccga aaatggttga attgaatatg tacaacggca
    40021 tacctcatct gttgacgccg gtcgtgacga accctagaaa agcagcgcaa gccttacaaa
    40081 aagtagtaca agaaatggaa gaacgatatg aaaaattcgc ggcaaccgga gtaagaaata
    40141 tttctggtta caatgaattt gttcaacaga aaaacttgga aaacggaacg aaacatccaa
    40201 ccttaccatt catcgtagtg atcgtagatg aattagctga tttgatgatg gtagccagca
    40261 atgaagttga agatgcgatt attcgtttag cgcaaatggc tcgggcagcg gggattcata
    40321 tgatcctagc tactcaacgt ccaagtgtgg atgttatcac aggaatcatc aaagcaaatg
    40381 taccttccag aatggcattt gcggtatcaa gcggtacaga ttctcggacg atcattgatt
    40441 ctaatggggc agaaaagctc ttagggcgag gagatatgct attcttgcca atgggtgaga
    40501 ataaaccgat ccgtgtgcaa ggagcattca tttctgacca cgaagtagaa cgggtcgttc
    40561 aatttgttac agatcagcaa gaagctcatt atgaagagaa aatgatgccg acagatgaag
    40621 ttgaaacgac aggtgctcca gaacagccac aagatgagct gtttgaagaa gcaaaagcac
    40681 ttgtcgttga aatgcaaacg gcaagtattt cccttctcca aagaagattt cggatcggtt
    40741 acaaccgagc agcacgttta gtagatgaac tggaagctca tggagttgtt ggcccgtcag
    40801 aaggaagcaa gcctcggaaa gtattcattg agcaggaaga aacatcagaa acgttgccgg
    40861 acgataatga catgtaaaac ttgtgtgatg aagggttgaa aaacgaataa ccaataaaaa
    40921 gacaaggcat gatgatacca aaagaaaaaa tggttttttt ccatcttttc taatacggta
    40981 tgatgtcttg tctttttagt ttttgtgaga ttaaagttct attcgattta tttgtgaagt
    41041 attgcattaa ggaaatgaat catgtatagt atgtagcgaa actttgagaa aaagagggaa
    41101 aatactttgg aaaaacagtc catttatggc ttgacgaatg aagaattaat caattggttt
    41161 atagaaaacg gcgagaaaaa attccgagct gcccaagttt gggaatggtt gtatcaaaaa
    41221 cgtgtaagta atttcacaga aatgacgaat ctatcgaaac aattgatcga aaaattatcg
    41281 gagcatttta tcatcaatcc tctaaaacag atggtcgtcc aagaagcttc ggatggtaca
    41341 gtaaaatatt tgtttgaact ccctgataaa aacatgattg agactgtact gatgagacaa
    41401 gaatatggct tgtcagtttg tgtgacgaca caagtaggat gtaatatcgg ctgtacgttt
    41461 tgtgcaagcg ggctgctcaa aaagaaccgt gatctgactg ctggagaaat tgttgcacag
    41521 atcatgatgg tccaacatta ttttgatgag cgcaatcttg gcgaacgcgt ttcacatgtc
    41581 gttgtaatgg ggatcggtga gccatttgat aattacgaca atgtaatgga ctttttgcat
    41641 atcatcaatg atgccaaagg attggcaatt ggtgctcggc acattacagt ttccactagt
    41701 ggattagcac acaaaatcaa ggaattcgct aataacggct tgcaagtcaa tctggctatt
    41761 tccttgcacg cgccgaataa tgaggtgcga acatcgatca tgcggatcaa ccgaagtttc
    41821 ccaattgaga aattgatgga agctgtggat gagtatttag agaaaacaaa ccgtcgaatc
    41881 acgtttgaat atatcatgtt gaatcaagta aatgatagac cagagcatgc gcaacaatta
    41941 gcagatctat taaaagataa gaaaaaatta gcatatgtca acttgattcc atataatcca
    42001 gtcagtgaac atgatcaata ttctcgcagt ccaaaagctg atgtattgag attttatgat
    42061 gtattgaaga aaaacggcgt aaactgtgtg atacgtaaag aacatggtac agatattgat
    42121 gcagcttgcg gacaattgcg aagcaagcaa atgaaaaaaa cagttgccaa ataatcgtat
    42181 cctactcgtt ttttcaatta ccataaaagg agcagttttc ttaattaagg aactgttcaa
    42241 cttaaataag agaaaagatt ctatagcgaa agctataaag ctttttttga caagaaaaac
    42301 cctcatcaag caaagaaatg tcctcaattt gatgacagcc tgcttgatgg gggttttagc
    42361 aattatttaa ctgattttct tatatccttg ctggctttct tgtgtagcat gataagtttc
    42421 tacgtcaaga atggcctgtt taaatttttc tcgggtgatt tcataaggca catgtttaat
    42481 atcatttgtt gccatggctt tatctaagat tgcttctaaa tcggtcgtga cattgagatt
    42541 catgtcggtt aacgatactg gaagttggtt ctttttcata aacacataga ttttatcaaa
    42601 caaatcgaac tgctgatcca tcatcaataa ggtcagtaca ccataactga cagatgctcc
    42661 gtgaaggtat ccctccattt tttctagaac agtcgtaccg taatagaatg agtgtgccat
    42721 agcagaatta tagttattgt taactaaaac agaaaccaat ccggtagtaa caatgatatc
    42781 cgaaacgact tgttcaattg catatgaggg acgatcgtcg gcactatctt tcaatgcttg
    42841 cgtaccataa gtcaagagac gatcaatgca catttttccg atttggattc ctaatctatc
    42901 cgtgtagcct agatcatcgc cacgagcaga aaaagtagat tcgacttctt tggataaagc
    42961 atcgccaata cctgcccata agtatttatc aggtgcttcg gcaataattg ctgtatcaat
    43021 gaaggtgtgt acaggtggtt ttgaaggaaa gaagagtccg tccattacgc cttcttcgtt
    43081 atagatgaca cataccgcag tgacgggcga acagttcgaa ccaatagtgg gaaaggtgaa
    43141 gactggttta gctagttttt caccaatgac ttttacagta tcgcaagcac ggccaccacc
    43201 gactgcaaac aggacgtctg ctttttggac actttcatgc tggatcaact gttcaatatt
    43261 tccataagtt gaattacccc cgtaccagag actggcgaca acctctattt cctgtgtcaa
    43321 agctactttt attttgtttt ctgcctttga aagtgcagtc ttccctccaa tcagcactac
    43381 ttttgtccca tattctttga cgattccatt tgctttttca tacgcatctg ttccaatggt
    43441 ataatttggt aagaaaacac tttgactcat attcatttcc ttctttcttc gttgaaacgg
    43501 gttaaaattt taaagagtga tcgatctgac tgcggctgta gatggagtag aggtgaacct
    43561 aagacaggat aagtgatgaa taaggtggca tgaacacctt gaaatcatca ataagtatcc
    43621 taaaaacgaa caagtacaaa aacattgaaa acgacaataa tgaatttttg ttcctcttag
    43681 tatttctacc gattcccttt gcttttttgt aattatctaa aaattctaaa aattaagatt
    43741 attatatgaa aacaggtttt atttgtcaat tcataattgg ttttttttaa agcaaaagat
    43801 aatctatatc atgatgaaca aacagagttt tttcctccaa ttgatggctt tcctgatctt
    43861 tcctattttg aataaagtgt ttctgtataa ttttttattt tataagtaag ataaataatg
    43921 aaagaataga gatactgatt ggtagctaaa gatttttgag cgagaacatt tcgttttttc
    43981 aaaaggactg ttagaataag caagaacaag ggggaagacg tatgataatc agtgaatttg
    44041 accgtaataa tccagtattg aaagatcagc tttctgattt actgagactg acttggccgg
    44101 aagaatatgg agacagctcg gcagaagaag tagaagaaat gatgaatcca gaacgaatcg
    44161 cggtagcagc ggtagaccaa gatgagttag taggatttat tggtgcaatc cctcaatacg
    44221 gtatcacagg ttgggaattg catccattag ttgtagaaag ctcccgacga aagaaccaaa
    44281 taggtactcg attagtcaat tacttagaaa aagaagtagc ttccagagga ggaatcacga
    44341 tttatttagg tacggatgat ttagaccatg gaacaacgtt aagtcaaacg gacctgtatg
    44401 agcatacatt tgataaagtg gcttccatcc agaaccttcg tgaacatccg tatgaattct
    44461 atgaaaaatt aggttataaa atcgtaggtg tcttaccaaa tgcaaatggc tgggacaaac
    44521 cggatatttg gatggcaaaa acgattattc ctcgaccaaa ttctcaataa taaaaagagg
    44581 ttgtgacaga agtaatcagc ttcaagaaat aagaaggaat ttacgaaaat tacttcttga
    44641 atttttgtga aattccaact tatttccgaa gaagctactt ttggaacacc gtgtac
//
LOCUS       DASHHB010000020        44336 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.020, whole genome shotgun sequence.
ACCESSION   DASHHB010000020 DASHHB010000000
VERSION     DASHHB010000020.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 44336)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 44336)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..44336
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.020"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1152
                     /locus_tag="U2K71_001168"
     CDS             <1..1152
                     /locus_tag="U2K71_001168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HEM7315267.1"
                     /translation="PSPVTGTVKNVIVPEGTVANVGDVLVEIDAPGHNSAPSTSAPSA
                     EAPKEKVETSGSASVVEAADPNKRVLAMPSVRQFAREKDVDISQVTATGKGGRVTKED
                     IENFLAGGPSSAPAKSEAPEAAAPKEAAPAAESKPAAPAKPFKSNLGDLEERVAMTPT
                     RKAIAKAMVNSKHTAPHVTLHDEVEVSKLWDNRKRFKEVAAANGTKLTFLPYVVKALT
                     ATVKKYPVLNASIDDANQEIVYKHYYNIGIATDTDHGLYVPNVKDADRKGMFAIADEI
                     NEKAKLAHDGKLSAEDMRNGTITISNIGSVGGGWFTPVINYPEVAILGVGTIAQQPIV
                     NAEGEIVVGRVMKLSLSFDHRIVDGATAQQAMNNIKRLLADPELLMMEG"
     gene            1160..2566
                     /gene="lpdA"
                     /locus_tag="U2K71_001169"
     CDS             1160..2566
                     /gene="lpdA"
                     /locus_tag="U2K71_001169"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HEM7315268.1"
                     /translation="MVVGDFAVELDTVVIGAGPGGYVAAIRAAEMGQKVAIIEREYIG
                     GVCLNVGCIPSKALIAAGHHYQESLDSEMFGVTSENVKLDFAKTQEWKENKVVHTLTS
                     GVGFLLKKHKVETIEGEAFFVDDHTLRVIHPDSAQTYSFNNAIIATGSRPIEIPGFKF
                     GGRVLDSTGGLALKEVPKKFVIIGGGVIGAELGGAYANLGAEVTILEGSPQILPTYEK
                     DLVKLVEDDFKKKGVTVVTNAMAKEAVDNGDSVTVKYAVDGKEESVTADYVMVTVGRR
                     PNTDDMGLEQAGVEVGERGLITVDKQGRTNVPNIYAIGDIVPGAALAHKASYEAKIAA
                     EAISGKKVAVDYKAMPAVAFTDPELASVGMTIKEAKDAGIEAKAYKFPFSGNGRALSL
                     GKTEGFIRLVTTIEDNVLIGAQIGGVGASDMVSELALAIESGMNAEDIALTIHPHPSL
                     GEIVMDASELALGLPIHI"
     gene            2728..3003
                     /locus_tag="U2K71_001170"
     CDS             2728..3003
                     /locus_tag="U2K71_001170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0223 family protein"
                     /protein_id="HEM7315269.1"
                     /translation="MQEYQYPLDLDWTTDEMVIVMKMWEDLERANEKGINNEAFLKTY
                     QQFKTVVKSIGEEKRLGREFEKASGYSLYRTVQQAKKNPNKTLRMNG"
     gene            3009..3776
                     /locus_tag="U2K71_001171"
     CDS             3009..3776
                     /locus_tag="U2K71_001171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HEM7315270.1"
                     /translation="MEQMIDHIKSWLEEAGEKIKSSQQTLTISQKSNRTDLVTNVDRN
                     IQNFLIEKIHSFDPEAKILAEEEGCNQLSDLDGRVFIIDPIDGTLNFVIEGENFCIML
                     AVYESGVGKLGFIYDVMRNELFWGGKGIGVYKNDEPLPRPANKSLSEGLLGVNSYLFG
                     HNKYGIREIAESSMGTRMYGCAGLELVAILKGNHIAYISNLSPWDYAAGNVLLEEFGM
                     VTSGLFGTPLTFEKREYYLAGTSCAYAEIADLLKKEG"
     gene            3944..5773
                     /gene="typA"
                     /locus_tag="U2K71_001172"
     CDS             3944..5773
                     /gene="typA"
                     /locus_tag="U2K71_001172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HEM7315271.1"
                     /translation="MNYRNDIRNVAIIAHVDHGKTTLVDELLKQSDTLDAHTQLQERA
                     MDSNALEKERGITILAKNTAVDYKGIRVNIMDTPGHADFGGEVERIMKMVDGVVLVVD
                     AYEGTMPQTRFVLKKALEQHITPIVVVNKIDKPSARPEHVVDEVLELFIELGADDDQL
                     DFPVIYASALNGTSSLSDDPADQEPTMAPIFDTIIEKIPAPVDNSDEPLQFQVSLLDY
                     NDYVGRIGIGRVFRGTIKVGDQVALIKLDGTVKKFRVTKLFGFFGLKRLEIQEAKAGD
                     LIAVSGMEDIFVGETVTPVDHQDALPILHIDEPTLQMTFLVNNSPFAGREGKFVTARK
                     IEERLMAELQTDVSLRVEPTNSPDAWTVSGRGELHLSILIENMRREGYELQVSRPEVI
                     EKEIDGVKCEPFERVQIDTPEEYMGSVIESLSLRKGEMQDMVHTGNGQIRLTFLTPAR
                     GLIGYSTEFLSMTRGYGIMNHTFDQYLPMLPGQIGGRHQGALVSIDTGKATTYSIMSI
                     EERGTVFVEPGTEVYEGMIIGENSRDNDLTVNITKAKQMTNVRSATKDQTSVIKKPKQ
                     LTLEESLEFLNDDEYCEVTPESIRLRKQILEKNAREKASKKKK"
     gene            6034..7806
                     /gene="recQ"
                     /locus_tag="U2K71_001173"
     CDS             6034..7806
                     /gene="recQ"
                     /locus_tag="U2K71_001173"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HEM7315272.1"
                     /translation="MNDLLKQYFGYDEFRPGQKEIIQKVIDQENVLGIMPTGSGKSIC
                     YQLPALLLDGLTVVVSPLISLMKDQVDAANQLGIPATFINSSLDGYETARRFQEIDRQ
                     QYRLLYIAPERFIMPDFIQAMKRWNVRMIAIDEAHCISQWGHDFRPSYLQMANQLDQL
                     PNRPVIVALTATATVQVAADIKRLLKIPENNHIQTGFERENLRFQVIKDQKKEQYLIE
                     YLKINKNQSGIIYAATRKEVDRLYHLLKKFDFSVGRYHGGLNENERTEMQEAFLYDRL
                     QLIVATNAFGMGINKSNVRFVIHYQIPGSLEAYYQEAGRAGRDGLSSEAILLFSPQDI
                     QVQKFFIQQSQREEGQKQKEYEKLKAMTEYVYIESCLQQYILNYFGETSSPCNRCGNC
                     LDDREIVEVTTEAQMVLSCLKRMGENYGKQMLMKVLAGSKEQKLQALGFGHLSTYGLM
                     KNQSQKETMQLIEYLISNGYLLTINGEYPVLKVTERGIQVLKGQESVYRKEPKKVQQL
                     SDEETDTLFEVLRELRTDLASEAGVPPYVVFSDSTLKEMSRIRPSSRLEMLQIKGVGQ
                     SKLDKYGEAFLSRIKNADSNVLDK"
     gene            7920..8222
                     /locus_tag="U2K71_001174"
     CDS             7920..8222
                     /locus_tag="U2K71_001174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlaN family protein"
                     /protein_id="HEM7315273.1"
                     /translation="MDSLSKKLAIQVLNDEAERIKRLIRNQNNSLCISQCKAFEEVVD
                     TQMYGFSRQVGFAVRIGVIGKNEGQLMLSELERELNQLYSEVYQEIYDKKEVGKEE"
     gene            8225..9388
                     /locus_tag="U2K71_001175"
     CDS             8225..9388
                     /locus_tag="U2K71_001175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEM7315274.1"
                     /translation="MPKKVKKRHLLDYSILIPYLILCVLGLIMVYSSTSYLLLENGQN
                     PSASVINQSIFWVLSLIAIALLYKMKTDVLKNQRLIMAAIAVLTILLLIVVFFGKEIN
                     GAKGWLQIAGFSIQPAEYLKIISIWYLSLTLSKRQNSVQKDFLGTVKRPLAMVIGLTA
                     LVAILPDFGNAAVIFLIILVLLLASGVNYVYTLIVGVGGFCLSTFTIWLINITNGKIL
                     PGRLQYIYNRFAIYQNPFSDELNKGHQLVNGYYAMFNGGLFGRGLGNSIQKKGFLQEA
                     QTDFIYAIVVEELGVIMGILILALLFFMIVRIVLVGIRSKDPFNSLLCIGIGAMFLIQ
                     VFVNLGGITGVIPLTGITFPFLSQGGSSLLMLSICVGFVLNISADEKRKSLGL"
     gene            9414..12842
                     /locus_tag="U2K71_001176"
     CDS             9414..12842
                     /locus_tag="U2K71_001176"
                     /EC_number="6.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate carboxylase"
                     /protein_id="HEM7315275.1"
                     /translation="MKKVLVANRGEIAVRVFRACTELGIKTVGIYAEEDEYSVHRFKA
                     DEAYLVGQGKKPIDAYLDIEGIISIAKECGADAIHPGYGLLSENLNFAQRCEEEGIIF
                     VGPKLHHLDIFGDKIKAKAAAIEAGIASIPGTDGPIASIDDALEFAKQYGYPIMIKAA
                     LGGGGRGMRVAHDEKSAREGYERAKSEAKAAFGSDEVYVEKYIANPKHIEVQILGDTH
                     GNVIHLFERDCSVQRRHQKVVEVAPCVSMNEQQRQKICQAAVQLMKYVGYVNAGTVEF
                     LVEGDDFYFIEVNPRVQVEHTITEMITDIDIVTTQLLIAQGLDLHKEIGLPQQEGIKL
                     NGSAIQCRITTEDPLNNFLPDTGKIDTYRSPGGFGVRLDVGNAYAGYVVTPYFDSLLV
                     KVCTHGATFETAIQKMERCLKEFRIRGVKTNIPFMLNVITHPEFQSGNAKTTFIDSTA
                     TLFEFPRLRDRGNKTMKYIGEITVNGFPGIESGEKPFYEEPRMPKDLITRTDYVTAKN
                     VLDAKGADALVEWIKGQENLLLTDTTFRDAHQSLLATRVRTKDFKQIARLTGEGLPEL
                     FSSEMWGGATFDVAYRFLNEDPWQRLRKIRSLMPNTLLQMLFRGSNAVGYSNYPDNVL
                     VEFVKEAAAQGIDVFRIFDSLNWTPQMEKSIQAVRDTGKIAEAAICYTGDINDPSRAK
                     YNVQYYKDMAKELEQLGAHIIAIKDMAGLLKPQAAYRLISELKETTDLPIHLHTHDTS
                     GNGIITYSAASKAGVDIVDVAMSAMSGNTSQPSMSSLYYALVNGPRLPEITIENAQKL
                     NHYWEDVRMYYKPFENGLNAPETEVYMHEMPGGQYSNLQQQAKAVGLGHRWDEIKQMY
                     HTVNLMFGDIVKVTPSSKVVGDMALFMVQNDLTEEDIYEKGETLSFPESVVTFFQGEL
                     GQPVGGFPKKLQKIILKGRPALNERPGLLAESVDFNEVKKELAEKIGYEPKQDEVLSY
                     LMYPQVFLDYQKAYNQFGDVTLLDTPTFFQGIRLGETINVQIERGKTLIIRLDEIGEP
                     DIEGNRVLFFNLNGQRREITIKDNSIISTVQTRLKAEPTNREQIGATMSGSVLDVLVK
                     KGDNVKKGDTLMITEAMKMETAIEARFDGEVAHVYVSSGDTISSGDLLIEVTEK"
     gene            12863..13987
                     /locus_tag="U2K71_001177"
     CDS             12863..13987
                     /locus_tag="U2K71_001177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315276.1"
                     /translation="MKRLGLFWLILLLVIVAFYMEPVLFPPKLEVKENSDQQISNQMT
                     TWKYQELKATGFSTYIGKPIDQLEKDFGQAYDRLTSGFGFETRYYENKASHLTFEANI
                     QDSRVTTVKVLQTGPADIAPFYLGMTMQDLTKITTIYTNFTFEYHDTEVGIELMEEDM
                     NYRPLIAFDNDTFAILFFDQTTNGLFSVVYLDKDSLLKLLPYQVFGEGLPHYQADKSA
                     DWESINRVKERKSTTLLNMLRREDELPIYNQTLAFQNHSQLLLHDFLTRPEEILSEER
                     MADWQKSLGSAQTTVKFSLTNDELEKLISKQKLENTSGLFIHPAIDATFSFLYWYSDP
                     YNHDRFMDPGTDSVGIAFSKENMLVLLQEENGESSDSSDR"
     gene            13977..14414
                     /locus_tag="U2K71_001178"
     CDS             13977..14414
                     /locus_tag="U2K71_001178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEM7315277.1"
                     /translation="MIVNEELFQLEDQCLQVAEMIKKSKTMASYLEHKQSMEESAKVC
                     QLKEAFLSKKEAFENVAAYGEYAPDYREKKRAVRKAKRQLDMDEHVAAFRYSETQLQS
                     LLDQIVQVLAEEVSNEIKVDAGSPFFETKKHFGCGGNCHAIRQ"
     gene            14398..14736
                     /locus_tag="U2K71_001179"
     CDS             14398..14736
                     /locus_tag="U2K71_001179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbG family protein"
                     /protein_id="HEM7315278.1"
                     /translation="MQLDNSEFEITKRRGLIVWVYSLKQLKNLKKFGLLHYVSRRMKY
                     VVLYLNEETLEETEERIRKLHFVRNVERSYRPDIEMNFAEKIGNKTVLKDEGFEIEEL
                     NTEIRLAEHL"
     gene            14855..15415
                     /gene="rsmD"
                     /locus_tag="U2K71_001180"
     CDS             14855..15415
                     /gene="rsmD"
                     /locus_tag="U2K71_001180"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HEM7315279.1"
                     /translation="MRIISGNYGGRRLKSLAGANTRPTTDKVKESIFNMIGPYFDGET
                     VLDLFAGSGGLAIEAVSRGCSHAVCVDKNYQALKIIKENIEITKEPEKFTTIKLDADK
                     AIERLAAEKQQFDYLFLDPPYAKQKIIDQIHKMEELGLFSKTAVIVCETDKTVELPET
                     VAGFHQIRKQIYGITAVTIYRKEGNQ"
     gene            15412..15903
                     /gene="coaD"
                     /locus_tag="U2K71_001181"
     CDS             15412..15903
                     /gene="coaD"
                     /locus_tag="U2K71_001181"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HEM7315280.1"
                     /translation="MKRALFPGSFDPFTKGHLDTVERAAKLFDEVVIGVFINTSKKSL
                     FPPEERMTLITKAVSHLPNVKVMHQENQLTVETAKEIGADALIRGIRSIKDFEYEREI
                     AQMNHHLYDELETVFLLAKPEYSHVSSSILKEVLHFGGDVSSYLPPVINEALAGKRES
                     NES"
     gene            15893..16936
                     /locus_tag="U2K71_001182"
     CDS             15893..16936
                     /locus_tag="U2K71_001182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HEM7315281.1"
                     /translation="MKAKQWLKTVLIIIFGMLIIGFVVVPLPYYVESPGATIDLKELI
                     TVNDKEDKGSGSFSLTSVGIRQATGFLALKAKMTPFEELISEEELTGGATNEEYNQMQ
                     QYYMDSSQNAAIEQALKLADKPYKMEFKGVYVMSVEKTSNFFGKLSVGDTVTKVDGKS
                     FQSTEAFMDYVKSQKVGQTIEISYLHNGEEKTASGDLIELPTDKKAGIGITLTDHTEI
                     ESDTDVRINSGSIGGPSAGLMFTLEIYEQVTGKNLRHGKQIAGTGTINSEGEVGRIGG
                     IDKKVASADKAGVEIFFAPDDTISDDVKKEYPEIKTNYEEAKAAAKKLNSSMKIVPVK
                     TVQEALDYLEKMK"
     gene            17095..17823
                     /locus_tag="U2K71_001183"
     CDS             17095..17823
                     /locus_tag="U2K71_001183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016925838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HEM7315282.1"
                     /translation="MKGVMNDFYWSFFEKRTMIEKISKKILASPKLIIWMSMIVLIFC
                     IASGFLLSNLLFYRETEETAAKEILSSFSSSGEDPSAEGNDVEEDARPKVMYTDIKGS
                     VKEPGIYSFSSEERVYDVLKRAGGLLEEADSDRINFSAKIEDQQVLYIPAVGEELPEH
                     LDQLASSEGKQPTADSEPPKININTATPSELQQIPGIGSVKAQEIIRFREENGSFQKV
                     EDLQEISGIGEKTVEKLKNFVTIK"
     gene            17939..18184
                     /locus_tag="U2K71_001184"
     CDS             17939..18184
                     /locus_tag="U2K71_001184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEM7315283.1"
                     /translation="MIVRKETLKKPMLNVYLQNKISGIHIMNTAVSGNNSQALRERFA
                     KDVLSYTADKVFILIGTNDLAEHKQLSKETYQKICSG"
     gene            18181..18399
                     /locus_tag="U2K71_001185"
     CDS             18181..18399
                     /locus_tag="U2K71_001185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase"
                     /protein_id="HEM7315284.1"
                     /translation="MIVQLKKDYSSDHAFFITPPAVDETKQKYRNNKLIKEYISVLTE
                     VAKRHDCEVSIFIRNFLNKWIRRIFIMG"
     gene            18535..19074
                     /locus_tag="U2K71_001186"
     CDS             18535..19074
                     /locus_tag="U2K71_001186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComE operon protein 2"
                     /protein_id="HEM7315285.1"
                     /translation="MLEKSSKACDTNKEKVTDKSHNERIPWDQYFMAQAVLLSLRSTC
                     TRLEVGATLVREKRIIAGGYNGAVSGDVHCIDEGCYVVDGHCLRTIHAEMNALLQCAK
                     LGIPTEGSEIYVTHFPCLACTKALLQAGVKGINYLHDYRNDPYAQALIEQLGVSVHQV
                     ALDSQHFSKLQNELTGKHV"
     gene            19067..21340
                     /locus_tag="U2K71_001187"
     CDS             19067..21340
                     /locus_tag="U2K71_001187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA internalization-related competence protein
                     ComEC/Rec2"
                     /protein_id="HEM7315286.1"
                     /translation="MSSVLERHVRNRLIFPAITSMIICLLYYTDNWGYRLLLAGIFLW
                     FSLKEGRFIAYLCILCSITVSISYRLQEYSFLEEKTTSAQITVFTDTIRANGNFLTME
                     GRLSENKQKLQLSYQVNTEAELKTWLNFRGKGIELTAEGEFISPAKKRNLYNFDQEAY
                     FRNHRISGRFQIQKIEQITMQNHWRNWLQRKRGSFISFVQSEFPQKTSVYINSLLWGY
                     KDAGFREPEEIFRESGLLHLFSLSGLHIQFYLGWLYYLFRRIGWPLHVSIIPLSLLTI
                     CYVSIAGSSISVLRASLLFLLRLLVKLLNKHYSTMDLFAVVFWLLLLFDPRFLFHAGG
                     QLSFFMSFLFILISFDGDWLKKTASHVLFTMITMPLIVWHFYEWPILGSLFTLLIAPV
                     FKFLFLPGVLFLTLFNRWLPETLLLLIEEGLILFERIIDFSSGSSLIIGRLPLVLSGM
                     YIFGLLICMDRLKEQPVKHLFYVGLFSFIFLTLPFLRFSSTIAFIDVGQGDSIFLQSP
                     FRKETILIDTGGQLHFEREKWAQGMIRPPAERNIVPYLKGQGISVIDKLILTHDDTDH
                     VGELPTLSQHFTIKKIYIGWGAGRNEPLQRHLSEAQKNGTEIIEIKHGDRISGYYDLY
                     VLTPFEKGEGRNEDSIGLWMEYNNRRFLFLGDLNQAMEKQLLLIYPNLKADVVKLGHH
                     GSRTSSNTDFLSHIEAKHGIISCGVNNRYGHPHSEVLETLEENQIKTWRTDQQGMITY
                     RWHPVFHPHGLVETMID"
     gene            21378..22424
                     /gene="holA"
                     /locus_tag="U2K71_001188"
     CDS             21378..22424
                     /gene="holA"
                     /locus_tag="U2K71_001188"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HEM7315287.1"
                     /translation="MVSNLSIQAELQKIRKEKLASCYLILGTEKFLQDQVRTEIVKKI
                     QLEGEDDLNFLSFDMENASLDEVVAEAETLPFFGDQRLVFVENPYFLTGEKVNNGIEQ
                     NTDLLTAYLKEPLESTVLVFFAPYEKLDERKKVTKQLKKTAIIINVQQLNEKEVRQYL
                     MNTLENSSIKMERSAIDLFLRLTDLDLSKMMRELQKLVLYGQNQQQITVREVEELVPK
                     TLEHNIFDMTQYILKGKTEQALRLYEDLVMQGEETIKINAILLSQLRLFLQTKFLVKI
                     GYQQANIAETLKIHPYRVKLAMQEVKKFDEHLLVQLFDQLVEMDYQIKTGQIEKELSF
                     QLFVLRTGQLLRNR"
     gene            complement(22513..22767)
                     /locus_tag="U2K71_001189"
     CDS             complement(22513..22767)
                     /locus_tag="U2K71_001189"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HEM7315288.1"
                     /translation="MPNIESAIKRVRTSENANVKNSSQTSAMRTAIKKFEDAVASGAD
                     NVDALYKEAVKAIDMAESKGLIHKNKANRDKSRLSKKIAK"
     gene            complement(23167..23436)
                     /locus_tag="U2K71_001190"
     CDS             complement(23167..23436)
                     /locus_tag="U2K71_001190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315289.1"
                     /translation="METFEGLLIDTYVLQEQPMMLTGINLQLEDRVINGLLKDRYLAK
                     YILRLPLNKYVLEVSGKWNRRNQLVIKKMLIKNIDEYIKIIGTPE"
     gene            complement(23906..24361)
                     /locus_tag="U2K71_001191"
     CDS             complement(23906..24361)
                     /locus_tag="U2K71_001191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF441 domain-containing protein"
                     /protein_id="HEM7315290.1"
                     /translation="MESWLFLGIILLVAILAKNQSLMIAAAFVLIMKLIPASRSFFPW
                     LQGKGINIGVTIITAAILIPIATGEIGLRDLLEAFKSPIGWVAVFCGGLVAVLSSKGV
                     GLIASDPQITVALVFGTILGVVFLRGIAAGPVIASGITYCILQVFALGK"
     gene            complement(24499..26267)
                     /locus_tag="U2K71_001192"
                     /pseudo
     CDS             complement(24499..26267)
                     /locus_tag="U2K71_001192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375354.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
     gene            complement(26464..27297)
                     /locus_tag="U2K71_001193"
     CDS             complement(26464..27297)
                     /locus_tag="U2K71_001193"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HEM7315291.1"
                     /translation="MFFANIIKAVILGIIEGITEWLPISSTGHLILADEFIKLDASSQ
                     FMSMFNVVIQLGAILAVVVLYFHKLNPFSPSKSTVEKKDTWTLWFKVIVACIPAIIIG
                     LPLDDWLEANFHKFLPVAIVLIVYGIAFIIVEKRNKNKTPRWTSLNDFTYQAALLVGL
                     FQVLALVPGTSRSGATILGSILIGASRFVATEFSFFLGIPVMFGASGLKIVKYLAEGN
                     SFKMEETVVLLVGTFVSFVVSVLAIKFLLSYLKKNDFTAFGWYRIILGIILIGYWFIA
                     M"
     gene            complement(27489..28193)
                     /locus_tag="U2K71_001194"
     CDS             complement(27489..28193)
                     /locus_tag="U2K71_001194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM7315292.1"
                     /translation="MTETTLYIVRHGKTMFNTIERVQGWCDTPLTKQGQEGIHYLGRG
                     LKNVDFSFAYSSDSGRAIETARIILGEHSKGKEIPYFIEKRIREWCFGSLEGGYDMEM
                     WGVIPRVLNFKTYDEMFTTEVSFEQIANAVYNLDTAGWAETYETLRDRVWTGFEDIAH
                     HCEKNGGGNVLVVSHGLTIAFLLNLIDPKQPVRAGLLNGSVTKVTYANGRFSIHGIND
                     TSYIDNGKTHSRDSFL"
     gene            complement(28216..28842)
                     /locus_tag="U2K71_001195"
     CDS             complement(28216..28842)
                     /locus_tag="U2K71_001195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM7315293.1"
                     /translation="MHIERLKTGMIEENCYLVYNEEALLIIDPGEDAERIKQEIKKTN
                     QQPVAILLTHTHYDHIGAVEPLRAYYQIPVYVSPLEQKWLGDPILNLSGLGRHDDIAD
                     VIVSPAEYEFEMKTYTLGNMSFRVVPTPGHSIGSVSFIFDDFVVSGDALFRGSIGRTD
                     LYTGNLEQLLHSIRTQLFVLPDEFAVYPGHGDATTIEQEKRTNPFFNR"
     gene            29168..31543
                     /locus_tag="U2K71_001196"
     CDS             29168..31543
                     /locus_tag="U2K71_001196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase"
                     /protein_id="HEM7315294.1"
                     /translation="MIKQSKISVRHLVEFILRRGSIDNRKKSNHTALEGARIHRKLQK
                     DAGENYEKEVFLKTTVQLDEYYLIVEGRADGIFKRDGIYYIDEIKTSEPRFEDLEPEQ
                     VELFFHQARVYAYIYSQEHDLQEINLQLTYFQTAEEVITRKVEHQTREQLETFFKKLT
                     EDYKEWLVFQENWRTVRNASLMALKFPHDEYRKGQRELAVAAYKTIRTKQKLFVEAPT
                     GTGKTISTLFPALKAVGEEEGEKIFYLTAKTITRQVAEDAMTALKDTGAEVKSVTLTA
                     KDKICFLDETTCNPDQCPYANGYYNRINEGLWDLLNHENQITREVIETYARKHTLCPF
                     ELSLDVSIWCDVIIGDYNYLFDPTVYLRRFFEDEKNEDYLFLIDEAHNLVNRSREMYS
                     AELSYEKTKRSKEAIPKEFKKLHRRFNKLLKEFDSIREIAKEDHWDYHHQKAPAESLV
                     KAGYQLSEKIKEWLAEFPEHPSQEQLLPYYFDLLHFLKVSEFYDDHYETTVEKTYRDL
                     IVKEFCIDPSLFLEQSLDKGKSSILFSASFSPLSYYQETLGGQKSLAYKLPSPFPEGN
                     QQVFIANYLETTYRKREHSLAKLVETIHAFADGKVGNYFVFFPSYQYLDLAVEAFRQQ
                     YPDSNVLIQETDMNEEERERFLTKFQTNPAETLIGFCVLGGIFSEGIDLRGDRLIGSM
                     IVGVGLPQMNHEQELIKSYYDEKEHLGFAYAYQLPGMNKVLQAGGRVIRGMADQGIVV
                     LADQRFSSFSYRRLFPQHWQTAREVRSVGELEEGIQRFWLSKEAVNQKNEE"
     gene            complement(31567..32058)
                     /locus_tag="U2K71_001197"
     CDS             complement(31567..32058)
                     /locus_tag="U2K71_001197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YajQ family cyclic di-GMP-binding protein"
                     /protein_id="HEM7315295.1"
                     /translation="MAKDASFDIVSEVNTEEVKNAIQQTTKELSNRFDFKDSTVEIKL
                     ENSNLVVLSDDDFKIEQIKDVLFGKLIKRNVPIKNIHFSESEHALGGKARQTADLVNG
                     IDRENAKKITTAIKNEKLKVKAQIQEDQIRVTGKNRDDLQAVIALLRKLDLPIELQFT
                     NYR"
     gene            32245..33165
                     /locus_tag="U2K71_001198"
     CDS             32245..33165
                     /locus_tag="U2K71_001198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HEM7315296.1"
                     /translation="MTAYHKNERLKKIVVILITGLTAAVALNYFLIPANVFSAGMNGI
                     AQIISSLLYEWFHIKIDTGTFIFLLNIPVFVLGFLKVGKRATILSFVNVVCVSVMTML
                     LPVGQITDNILMNALVGGVLLGLGVGLSLKMGFTTGGMDIISLVLSQTTGKTVGNYML
                     ILNGIIVVIAGFFFNWESALYTIISIYAMTQVIDAIHTSHQKVTAMIVTVKPEEVTEE
                     LAKRMVRGMTLLPSVGGYSKKQGNMIMMVITRYEMYDLDQIVHEIDDDAFINILPTHS
                     VFGRFANENEQKMYRTTGVLPEIKNRSQKK"
     gene            33319..33663
                     /locus_tag="U2K71_001199"
     CDS             33319..33663
                     /locus_tag="U2K71_001199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-sulfur cluster assembly factor"
                     /protein_id="HEM7315297.1"
                     /translation="MSEANQERSGQEIELIKERILAALEMVIDPELGIDIVNLGLIYD
                     IEFNPENGETVIKMTLTTMGCPLADILTESIHDALKEVPEVTKAEVKLVWYPAWTTDK
                     MSRYARIALGIR"
     gene            complement(33853..34353)
                     /locus_tag="U2K71_001200"
     CDS             complement(33853..34353)
                     /locus_tag="U2K71_001200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7315298.1"
                     /translation="MKLIKLWEADLKKAYQLQTSFGPNESGFINNAYGYTFAEFLDYV
                     KKCEKTSLGIDLPESYVPATVFILESDEHEYVGIFNLRHKLNETLRNGAGHIGYGISP
                     NYRKKGYATQGLALVLQEAKKIGIDEALLSVNKKNLGSLKAQQKNGAAIYREDEKEYY
                     TKIKLM"
     gene            34881..34964
                     /locus_tag="U2K71_001201"
     tRNA            34881..34964
                     /locus_tag="U2K71_001201"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:34915..34917,aa:Leu,seq:cag)
     gene            complement(35288..35926)
                     /locus_tag="U2K71_001202"
     CDS             complement(35288..35926)
                     /locus_tag="U2K71_001202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM7315299.1"
                     /translation="MTSLKTLLFGTTLAAGAAFFMGTTAHADEAYTVQSGDTLSTISQ
                     KYVGDNSLINAIAESNSISDINLIYSGQQLTIPTEGSAQAAAEPQAAVQEAPVQAEPV
                     QAEQPVVQETVQTETQAAPVAETQPAPAVTETAATPASTSSAKEWIAQKESSGSYTAT
                     NGRYIGRYQLDSSYLNGDYSAANQERVAEQYVTSRYGSWEAAKAFWEANGWY"
     gene            36261..36695
                     /locus_tag="U2K71_001203"
     CDS             36261..36695
                     /locus_tag="U2K71_001203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM7315300.1"
                     /translation="MSKRYQNIMVAVDGSRQSIKAFAEALDLAKDNEAHLFIVSIINK
                     VELTHSAYAFSKIYADEKQRTEVAMLKKINDAKEYGIAEIHAIVETGDPRNLIANVIP
                     GQENIDLIVMGATGKGAIQQALVGSTASYVVSHAPCSVLVVK"
     gene            36718..37038
                     /locus_tag="U2K71_001204"
     CDS             36718..37038
                     /locus_tag="U2K71_001204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315301.1"
                     /translation="MVRPQEVRAPKEKIEILAIIEDGTQTKKGYSIALVKWKGKKGVA
                     LRWDGDNQQDKGFPVTANGYHPAWFVLPDKFMELYSYDYARAAASIKALDKLTEEQNE
                     MEEK"
     gene            37179..37370
                     /locus_tag="U2K71_001205"
     CDS             37179..37370
                     /locus_tag="U2K71_001205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315302.1"
                     /translation="MEAGKVLQEVKALCADKKYEEAKSLIEKNKENLEEKYPIAQEFI
                     ELKQGTILHRFKHFFGITE"
     gene            complement(37441..37698)
                     /locus_tag="U2K71_001206"
     CDS             complement(37441..37698)
                     /locus_tag="U2K71_001206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose uptake protein"
                     /protein_id="HEM7315303.1"
                     /translation="MIAIGQFVFYIPFFIMISILFYYIKWTKKKFSVLLASLPAVYFT
                     YQIFSFRHWETTSVLITHIIELTLSVIFLIIWIYFLYKNQN"
     gene            37856..38119
                     /locus_tag="U2K71_001207"
     CDS             37856..38119
                     /locus_tag="U2K71_001207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315304.1"
                     /translation="MDHVVLEIDLHLINNIRVIYYVVSNSVEQRVLTNKINGILAKKD
                     VHRFNNGEGSYYSIPVEKIIYTTVKVREDLDTKKAYEPIFTTY"
     gene            38143..38331
                     /locus_tag="U2K71_001208"
     CDS             38143..38331
                     /locus_tag="U2K71_001208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315305.1"
                     /translation="MNPYEQLIQDLIDGKIEKIDVKREELMAFREAWLKLENRKYVVG
                     KASLNGNVTYHYDPTRLF"
     gene            complement(38414..38599)
                     /locus_tag="U2K71_001209"
     CDS             complement(38414..38599)
                     /locus_tag="U2K71_001209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315306.1"
                     /translation="MNQEELFQKVKEMIKNGNFDGAKRFIEEHKEQLGPYKEKAQNLL
                     KDVNIDSVKNKFKNLFK"
     gene            38814..39185
                     /locus_tag="U2K71_001210"
     CDS             38814..39185
                     /locus_tag="U2K71_001210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM7315307.1"
                     /translation="MKVIKYLIGLAAISGILLFGLKYYTEGSYGEIPGILDQLNPLVE
                     KGEVYVKTQKPEEVNEYGTARYVQKAADANGKERTVEFNGLSVLKENHYLKISNKGAH
                     IETYEEVPREKVPEKALAIIG"
     gene            complement(39335..39407)
                     /locus_tag="U2K71_001211"
     tRNA            complement(39335..39407)
                     /locus_tag="U2K71_001211"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(39372..39374),aa:Gln,seq:ctg)
     gene            complement(39453..39881)
                     /locus_tag="U2K71_001212"
     CDS             complement(39453..39881)
                     /locus_tag="U2K71_001212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315308.1"
                     /translation="MEKFTHKKMDPNEIPIIFVRDCKGNVQGKVSINEWNERRRPATL
                     NELEIKLYRQSLVYYADQEYEKATDLLKFLIARTEYTRFEYIERLANIYHIMNEPVKE
                     YQLLDTVLSVAELIALPAGLEKKLVRRLLRVKQQLSDQEK"
     gene            complement(39941..40699)
                     /locus_tag="U2K71_001213"
     CDS             complement(39941..40699)
                     /locus_tag="U2K71_001213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HEM7315309.1"
                     /translation="MKKKTFKKLLFLGGTAALLLSGTSYYLHIHSPEHTMNTAQKSST
                     QKTKKTKEKAEVLLDVPLESQFDSPSLENGCEVTSLSMLLSFYGYETTKNQLAEQLDY
                     VPVFNADGTHGDPNEGFVGDISGGDWAMVVYVPPVASLAQQIVQTDYHTFPKTDAQLD
                     DIKKALSKGNPVWTSVTINFEVPDETDFMTWTTNNGEVRVTPLVHACVVTGYDKENIY
                     VNDPYGVKNRAVPINDFSAIFTAMGGQMLTLEKS"
     gene            40994..41191
                     /locus_tag="U2K71_001214"
     CDS             40994..41191
                     /locus_tag="U2K71_001214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315310.1"
                     /translation="MTEEMINLGEQYACKPIGFTKTVIGEVVSKMTNCAVVKVAQCAA
                     EDQELLDEKASMVVAKYDTFE"
     gene            41297..41779
                     /locus_tag="U2K71_001215"
     CDS             41297..41779
                     /locus_tag="U2K71_001215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315311.1"
                     /translation="MVTFFTVLLFLFVVVMFLFLWLVRKEIIYRTVRNRWVYLVIPFL
                     VSLVIWYTLISHPTADELAKGILSAMIFISFLLDSRGITEEGLVLNSFDKKGVPFSEI
                     NKIVLYQPKGSKIVKMNFFRNGWRGPMLKFSASLEELVPFLSQRLNDEAEIDIMIDPE
                     "
     gene            complement(41837..42433)
                     /locus_tag="U2K71_001216"
     CDS             complement(41837..42433)
                     /locus_tag="U2K71_001216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEM7315312.1"
                     /translation="MTTFTDTLKNRRSIYHLGRNVSLSNEELTTLIKEAIKESPTAFN
                     AQSTRAVILFGDAHEKLWEITEEALRPLTPAEAFPNTQNKLAGFKNGYGTVLFFKDTD
                     VVKGLQEQFELYADNFPDWSEQSNGIATANTWVALVDKGLGANLQHYNPVIDEAVAKE
                     WNIPSNWKLRSQLVFGSPETPAEEKEYMNDADRFRVFG"
     gene            42820..44286
                     /locus_tag="U2K71_001217"
     CDS             42820..44286
                     /locus_tag="U2K71_001217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide MFS transporter"
                     /protein_id="HEM7315313.1"
                     /translation="MEKQQDKGFFGQPKGLSTLFFTEMWERFSYYGMRALLIYYMYDT
                     VANGGLGLPRATALAIMSIYGSLIYMSSIVGGWVSDRVLGAKRTVFFGGVFIMLGHIV
                     LALPFGVSTLFVSMALIIIGTAFLKPNVSGMVGHLYSKTDLRRDAGFSIFYMGVNLGA
                     LLAPLIVGTIGQEYNYHLGFSLAAIGMFFGLLQYIIQGRKTLDGVGTTPPNPLTTEER
                     KGFIRNLIIALVVIAAVFGGAQLTGHLTIDFFVNTISVLGIILPLYYFIKMLTSKDVT
                     AEEKPRVWAYIPLFLAAIIFWSLQEQGSSILALFASERTQSTLFGVTIQASWYQSLNP
                     IFIFILTPVFVTMWTKLGERQPSTVVKFSLGLLFAGLSYLLLMLPGMLYGTSGKVSPL
                     WLLGSFFIVVIAELCLSPVGLSVTTKLAPRAFESQTIAIWFLADASSQAINAQIARFY
                     TPGTESAYFGIIGAVAIVGGILLFTVKEPIKKLMGSIH"
BASE COUNT        14646 a         7600 c         9080 g        13010 t
ORIGIN      
        1 ccgtcacctg tcacagggac agttaaaaac gtgattgttc cagaaggcac tgtagcaaat
       61 gttggggatg tattagtaga aatcgatgct cctggacaca atagtgcacc gtcaactagt
      121 gcgccatcag ctgaagcacc aaaagaaaaa gtggaaacat caggttcagc aagtgtcgtt
      181 gaagctgccg atccaaataa acgtgtattg gctatgccat ctgttcgtca atttgcccgt
      241 gaaaaagatg tagatatctc acaagtaact gcaactggaa aaggtggcag agtaacaaaa
      301 gaggacatcg aaaacttctt ggcaggtggt ccaagcagtg caccagcgaa atcagaagcg
      361 ccagaagcgg cagcaccgaa agaagcagct cctgcagcag aaagcaaacc agctgcacca
      421 gcaaaaccat tcaagtccaa cttaggagac ttggaagaac gtgtagcaat gacgccaaca
      481 cgtaaagcta tcgctaaagc aatggtcaac agtaagcata cagctccaca cgtgacattg
      541 catgatgaag tagaagtgtc taaactatgg gataatcgga aacgatttaa agaagttgct
      601 gctgcaaatg gtacgaaact tactttcttg ccatatgtcg tgaaagcttt aacagcgact
      661 gttaagaaat acccagtatt gaatgcatct atcgacgatg caaaccaaga aatcgtttat
      721 aaacattact acaatatcgg tattgcaact gatacggatc acggactata tgtaccaaat
      781 gtgaaagatg cagaccgcaa aggaatgttt gctattgcag acgaaatcaa tgagaaagca
      841 aaattagctc atgacggcaa actatctgca gaagatatgc gcaatggaac aattacgatc
      901 agcaatatcg gatctgtagg tggcggttgg tttacaccag tcatcaacta tccagaagta
      961 gctattttag gtgtaggaac gattgcacaa caaccaatcg taaatgctga aggcgaaatc
     1021 gttgtcggtc gtgtgatgaa actgtcatta agtttcgatc accgtatcgt agatggagca
     1081 actgcgcaac aagcaatgaa taatatcaaa cgtcttttag ctgatccaga gctattaatg
     1141 atggaaggat gataaacaaa tggtagttgg agattttgct gtagaattag atacagttgt
     1201 aattggtgcg ggacctggtg gctatgtagc tgccatccgc gctgctgaaa tgggacaaaa
     1261 agtagcaatc attgaaagag agtatattgg cggtgtttgt ttgaatgtcg gctgtattcc
     1321 ttcaaaagca ctgatagctg caggacacca ttatcaagag tcactagatt ctgaaatgtt
     1381 tggagtaact agtgaaaacg tcaaactaga ttttgcaaaa acacaagaat ggaaagaaaa
     1441 caaagttgtc catacgttga cttctggtgt tggtttccta ttaaaaaaac ataaggttga
     1501 aacaatcgaa ggagaagcgt tcttcgtaga tgatcacaca ctacgcgtca ttcatccaga
     1561 ttcagctcaa acttattcgt tcaataacgc aatcatcgca acaggttccc gtccaatcga
     1621 gattccagga tttaaattcg gaggacgtgt gttagattct actggcggat tagcattgaa
     1681 agaagtacct aagaaatttg tgatcatcgg tggcggtgtg attggtgccg aattgggcgg
     1741 agcgtacgca aacttaggag cagaagtaac gattcttgaa ggctcaccac aaattttacc
     1801 aacttacgaa aaagatctag taaaattagt agaagatgat ttcaagaaaa aaggcgtaac
     1861 agtcgtaacg aatgcaatgg cgaaagaagc agtcgataac ggtgacagcg tgacagtgaa
     1921 atatgcagtg gacgggaaag aagaatccgt tactgctgat tatgtgatgg tcactgtagg
     1981 tcgtcgtccg aatacagatg atatgggctt agaacaagcg ggtgtcgaag taggagaacg
     2041 aggattgatc acggtggata aacaaggacg tacgaacgta cctaacatct atgccatcgg
     2101 tgatatcgtt ccaggggcgg cattagcaca caaagccagt tatgaagcaa aaatcgctgc
     2161 tgaagcaatc tctggcaaaa aagtagctgt agattataaa gcaatgccgg cagtagcctt
     2221 cactgatcct gaattggctt ctgtaggtat gacgatcaaa gaagcaaaag atgctggtat
     2281 cgaagcaaaa gcatataaat tcccattttc agggaacggt cgggcattat cgcttggtaa
     2341 aacagaagga ttcatccgtc tagttacgac gatcgaagat aacgtgctta ttggcgcgca
     2401 aatcggtgga gtcggcgcaa gtgatatggt ctctgaatta gctttagcaa tcgaatcagg
     2461 aatgaatgct gaagatatcg ccttgacgat tcatcctcat ccatctcttg gcgaaatcgt
     2521 catggatgct tcggaattag cattaggttt accaatccat atttgatttg aataatacag
     2581 gcgaaacgca actgaaaaat cagttgcgtt ttgtttttta gaattatcaa taatattatt
     2641 atgtaaactt aaaaatatga caacttcttg gtttattacc acttcctatg ctacactgaa
     2701 gaggaatatg aaataggaga gttgaagatg caagaatatc agtatccact tgatttagat
     2761 tggacaacag acgaaatggt cattgtaatg aagatgtggg aagatctgga acgtgcgaat
     2821 gaaaaaggga taaacaatga agcttttttg aagacttatc agcagtttaa aactgttgtc
     2881 aaatccattg gtgaggaaaa acgattggga cgggaatttg aaaaagcttc cggttattct
     2941 ttatatcgta cagtgcagca agcaaaaaaa aatccaaaca aaactttgag aatgaacggg
     3001 tgagataaat ggaacagatg attgatcata tcaagtcctg gcttgaagaa gctggtgaga
     3061 aaatcaaatc ttctcaacaa acattaacaa tctcgcaaaa atcaaatcga acggatctag
     3121 taacaaatgt cgatcgaaac atccagaatt ttctaatcga aaaaatccat tcctttgatc
     3181 cagaggcgaa aatattggca gaagaagaag ggtgtaatca attatcggat ctcgatggaa
     3241 gagtctttat catcgatcct attgatggca ccttgaattt tgtgattgaa ggggaaaatt
     3301 tttgtattat gctcgctgtt tatgaatccg gcgttggaaa gcttggtttc atttatgacg
     3361 tgatgagaaa tgaacttttt tggggtggaa aaggaatagg cgtgtataaa aatgatgagc
     3421 cattgcctag accagcaaat aaatccttat cggaaggctt acttggtgtg aatagttatc
     3481 ttttcggtca taataaatat ggaatccgag aaattgcgga aagcagcatg gggacacgta
     3541 tgtacggatg cgctggtctt gaactagtgg caatcttgaa aggaaaccac attgcttata
     3601 tttcaaacct tagcccttgg gattatgcag cgggaaatgt cttattggaa gagtttggta
     3661 tggtaacaag cggtcttttt ggtactcctt tgacatttga aaaaagggaa tactatttag
     3721 cagggacttc ttgtgcatat gcagaaatcg cagacctttt gaaaaaagaa ggctgaaaaa
     3781 aaggtagaaa tcattcacaa aaatgataaa aacgagcaaa agattaattt tctcgaaaaa
     3841 atgaaaacat tttactatat ttcatgaaaa aagtctgcta taattagcag tcgagtaaaa
     3901 tttgccctgt cataaagaac caataaaaaa ggagttagtt acattgaatt atagaaacga
     3961 tatccgtaac gtcgcaatta ttgcccacgt tgaccacgga aaaacaacat tagttgatga
     4021 actattaaaa caatcagata cattagacgc acatacgcaa ttgcaagaac gtgcgatgga
     4081 ctcaaatgcg ctagaaaaag aacgtggtat cacgatttta gccaaaaaca cagctgtaga
     4141 ctataaaggt attcgcgtaa acatcatgga tactccagga cacgcggact tcggtggcga
     4201 agtagaacgg atcatgaaaa tggttgacgg cgttgtgttg gtcgtagatg cgtatgaagg
     4261 aacaatgcct caaacacgtt ttgtattgaa aaaagcactg gaacaacata tcacaccaat
     4321 cgttgtcgta aacaagattg acaaaccttc tgctcgtcct gagcatgtcg tagatgaagt
     4381 tcttgaacta ttcatcgaat taggagcaga tgatgatcag ttagactttc ctgtgattta
     4441 tgcatctgca ctaaatggta cgtcaagctt gtcagatgat ccagcagatc aagaaccaac
     4501 catggcacct atttttgata caattattga aaaaatccct gctcctgtcg ataacagcga
     4561 cgaaccatta caattccaag tttcattact tgactacaac gattatgtag gtcgtatcgg
     4621 aatcggacgt gtatttagag gaacaatcaa agtaggagac caagtagcat taattaaatt
     4681 agacggaaca gttaagaaat tccgtgtaac gaaattattt ggtttctttg gactaaaacg
     4741 tctagagatt caagaagcaa aagctggaga cttgattgct gtctcaggta tggaagatat
     4801 ctttgtcggt gaaacagtta ctcctgtgga tcatcaagat gcgttaccta tccttcacat
     4861 tgacgaacca acattgcaaa tgactttctt agttaataat tcaccatttg ccggtcgaga
     4921 aggtaaattc gtaaccgctc gtaagattga agaacgtttg atggcagaac ttcaaacaga
     4981 tgtatcctta cgagttgaac caacaaactc tccagatgca tggacagttt ctggtcgtgg
     5041 agaattgcat ttgtctatct tgattgaaaa catgcgccgt gagggatacg aattgcaagt
     5101 atctcgtcca gaagttattg aaaaagaaat cgacggtgta aaatgcgaac cgtttgaacg
     5161 cgtgcaaatc gatacaccag aagaatatat gggtagtgtt atcgagtcct tgagcttacg
     5221 taaaggtgaa atgcaagata tggtccatac aggaaacgga caaatccgtt tgacattctt
     5281 aacacctgcc cgcggtttga ttggttattc aactgaattc ctttcaatga cacgtggtta
     5341 cggaatcatg aaccatactt ttgaccaata tctgccaatg cttcctggtc aaattggcgg
     5401 acgtcaccaa ggggcgcttg tttcgatcga tacaggaaaa gcaacgactt attctatcat
     5461 gagtatcgaa gaacgcggta cagtcttcgt agaaccaggt acagaagtat atgaaggaat
     5521 gattatcggg gaaaacagcc gtgataatga tttgactgtc aacatcacga aagcaaaaca
     5581 aatgacaaac gtacggtctg ctactaaaga ccaaacatct gtcatcaaaa aaccaaaaca
     5641 attgacgttg gaagaatctt tggaattctt gaatgatgat gaatattgcg aagtaacacc
     5701 agaaagcatt cgcttgagaa aacaaatttt agagaaaaat gctcgtgaaa aagcatctaa
     5761 aaagaaaaaa taatctgtaa aaggatgaag agagaaacca gcttgttata aaaaatagct
     5821 ggtttctttt tttgtccaac tcttttctgt ggagaagtca tctttgtcaa tggacataaa
     5881 ttcagaaaat acgccgaaag acgtttaaaa gacttttaaa aaacttattt ctagagagtt
     5941 tttgtttcat aataagataa tgtttcacga aaggagatca acttgtcctt cgaagaaaat
     6001 cgaagtaaaa tggcaagaga aaggagcggg aagatgaacg acttattaaa acaatacttt
     6061 ggctatgatg agtttagacc tggtcaaaaa gaaattatcc agaaagtcat tgatcaggaa
     6121 aatgtattag gaatcatgcc taccggaagt gggaaatcaa tctgttacca gcttccagct
     6181 ttactattag atgggcttac tgtggttgtg tcccctttaa tctctttgat gaaggatcag
     6241 gtggacgcgg cgaatcaatt aggtataccc gccactttta tcaatagctc gttagatggt
     6301 tacgaaacag ctagacgctt ccaagaaatc gatagacagc agtaccgttt actttatatc
     6361 gctcctgaac gttttatcat gccggatttt atccaagcaa tgaagcgatg gaacgtccgt
     6421 atgatcgcta ttgacgaggc acattgtatc tctcagtggg ggcatgattt ccgcccaagt
     6481 tatttacaga tggcgaacca gctagatcaa ctgccaaatc gtcctgtaat tgttgctttg
     6541 acagctacag cgactgtgca agtagctgct gacataaaaa gacttttaaa aattccagaa
     6601 aacaaccata ttcagactgg ttttgaacga gaaaatctac gatttcaagt aattaaagat
     6661 caaaaaaaag aacaatatct aatcgaatat ttaaagatca acaaaaatca atctggtatc
     6721 atttatgcag caacgagaaa agaagtagac cgtctctatc atttgcttaa aaaattcgat
     6781 ttttccgtgg gtagatatca tggtggtcta aatgagaatg agcgaacaga aatgcaagaa
     6841 gcctttttat acgatcgctt gcagctgatt gtagcgacaa atgcatttgg tatgggtata
     6901 aataaaagca atgtccgttt tgttatccat tatcaaattc ctggctctct agaagcatac
     6961 tatcaggaag ctggtcgggc gggaagagat ggtctttcca gtgaagccat cctgcttttc
     7021 tctcctcaag atatccaagt ccaaaagttt tttatccagc agtcccaaag agaagaaggg
     7081 caaaagcaaa aagaatatga aaaattaaaa gcgatgacag aatacgtgta tattgagtcc
     7141 tgtcttcagc aatacatcct aaactatttt ggcgagacaa gcagtccgtg caaccgttgt
     7201 ggtaactgtt tagatgacag agaaatagta gaagtaacga cagaagcaca gatggttctt
     7261 tcttgcttaa aacgtatggg tgagaactat ggtaaacaaa tgctgatgaa agtcttagca
     7321 ggatcgaaag aacaaaagct gcaagcttta ggttttggac atttatctac ctatggactg
     7381 atgaaaaacc aatcgcaaaa agaaacgatg cagttaattg aatatttgat atcgaatggc
     7441 tatcttctga ccatcaatgg tgagtatcct gttttgaaag taacagaaag agggatacaa
     7501 gtattgaaag gacaagaatc tgtttatcga aaggaaccca agaaagtcca gcagctatcg
     7561 gatgaagaaa ccgatacact atttgaagtg ctgcgggaac tgcgaacgga tctagcatca
     7621 gaagcaggcg ttcccccgta tgtcgtattt tcagattcca cattgaaaga aatgagccga
     7681 atacgtccat caagcagatt ggagatgctt cagataaaag gagttggcca aagcaagctt
     7741 gataaatatg gcgaagcctt tttatctcgg ataaaaaatg cggactctaa cgttctggat
     7801 aagtagaaag aacgaatacg taaaaatgaa taaaaacaac tggttaaatc tgtttttttt
     7861 gctccttatg aaaatcaaat atgttataat taaggaatta gaatgggagg taagcacaga
     7921 tggattcttt gtcgaaaaaa cttgcgatcc aagtattaaa tgatgaagca gaacggatca
     7981 aacgattaat ccgaaatcaa aacaacagtc tctgtatctc acaatgcaaa gccttcgaag
     8041 aagtggtaga tacacagatg tacggctttt cccggcaagt cggttttgct gtccgtatag
     8101 gagtaatcgg aaagaacgag ggccagctga tgttaagtga gctagaacga gaattgaatc
     8161 agttatatag tgaggtttac caagaaattt atgataaaaa agaagttggc aaggaggaat
     8221 agacttgcct aagaaagtga aaaaaaggca tctattagac tatagcatct tgattcccta
     8281 tttaatttta tgtgtattgg gattgatcat ggtatatagt tctacgtcgt atctattatt
     8341 agaaaacgga caaaatccgt ccgcatcagt cattaaccag agtatttttt gggtgcttag
     8401 cttgattgct atcgcactac tttataaaat gaaaacagat gttttgaaaa accagcgttt
     8461 gatcatggca gccatcgcag ttttgacgat tttgctgctg attgttgtgt tttttggtaa
     8521 ggagatcaat ggggcgaaag gttggctgca aattgcagga ttctcgatcc aacctgccga
     8581 atacctaaaa atcatttcta tctggtacct gtctctgact ttatctaaaa gacaaaacag
     8641 tgtacaaaaa gattttcttg ggactgtgaa acgtccactt gcaatggtta tcggacttac
     8701 tgcattagtc gctattcttc ccgattttgg taatgcggca gttatctttt tgattatttt
     8761 ggttctgctt ttagccagcg gagtcaatta tgtgtatact ttgattgtag gagtaggagg
     8821 cttttgtcta agtactttta cgatctggct gatcaatatc acaaacggaa agatcttacc
     8881 cggacgttta cagtatatct ataatcgatt tgctatttac caaaatcctt tctcagacga
     8941 attaaataaa gggcatcaac tagtcaacgg ctactatgcc atgttcaacg gaggactatt
     9001 tggcagggga ttaggtaaca gtatccagaa aaaaggattt ttacaagaag cacaaaccga
     9061 ttttatttat gcaatcgtag tggaagaact gggagtgatt atggggattt tgatattagc
     9121 gttgctattc tttatgattg tgcgcattgt ccttgtaggt atccgttcaa aagatccgtt
     9181 caattcgctt ttgtgcattg ggatcggcgc aatgtttttg atccaagtat ttgtcaatct
     9241 tggaggaatt actggcgtta ttcctttgac agggatcacg ttcccatttt taagtcaagg
     9301 aggctccagt ttgctgatgc tttcgatttg tgtcggcttt gtcctcaaca tcagtgcgga
     9361 tgaaaaacgc aagagcttag gtttataata aaaaaagatg taggagtggt tcgatgaaaa
     9421 aagttttagt tgctaacagg ggtgaaatcg ctgtacgtgt atttcgtgct tgtacagaat
     9481 taggaattaa aactgttgga atctatgcag aagaagatga atattccgta caccgtttca
     9541 aagcggatga agcatattta gtaggacaag gaaaaaaacc gattgatgcg tatctagata
     9601 ttgaagggat catttctatc gcgaaagaat gcggagctga cgctattcat ccgggttacg
     9661 gtcttttgtc ggaaaacttg aattttgcgc aacggtgcga agaagaaggc atcatttttg
     9721 ttggacccaa actgcatcat ttagatattt ttggagataa aatcaaagca aaagctgcag
     9781 ctatcgaagc ggggattgct tcgattccag gcacagatgg accaatcgct tctatagacg
     9841 atgccttgga gtttgcgaaa caatacggtt atccgattat gatcaaggca gctctaggcg
     9901 gcggcggacg cggcatgcgt gtagctcacg atgaaaagag tgcaagagag ggctacgaac
     9961 gtgcgaaaag cgaagcaaaa gcagcctttg gtagtgatga agtgtatgtt gaaaaataca
    10021 tcgctaatcc taagcatatc gaagtacaga ttctcgggga tacgcatgga aacgtcatcc
    10081 atctttttga aagagattgt tctgttcagc gccgtcacca aaaagtagta gaagtagcgc
    10141 cttgtgtctc tatgaatgaa cagcaacgtc aaaaaatctg ccaggcagct gtccaattga
    10201 tgaaatatgt cgggtatgtc aacgcaggta cagtagaatt tcttgttgaa ggagatgatt
    10261 tttactttat cgaagtcaac cctcgcgtcc aagtggaaca tacgatcaca gaaatgatta
    10321 cagatattga tatcgtaaca acgcaactgt tgattgctca aggtttggat ctgcataaag
    10381 aaatcggatt gcctcagcaa gaaggaatta aattaaatgg ttcagccatt caatgccgaa
    10441 tcactacaga agacccgttg aataactttt taccagacac agggaaaatc gatacgtatc
    10501 gttcacctgg tggttttggc gtacgtctgg atgtcggaaa tgcttacgcg ggctatgtag
    10561 tgacaccgta ttttgattca ttgctagtaa aagtatgtac gcatggtgct acctttgaaa
    10621 cagccattca gaaaatggaa cgttgcttga aagaatttag aatccgtgga gtgaaaacaa
    10681 acattccatt tatgctcaat gtcatcacac atcctgaatt ccaatcagga aatgcaaaaa
    10741 caaccttcat tgatagtaca gcaacattat ttgaatttcc tcgactacga gaccgcggaa
    10801 acaaaacgat gaaatatatc ggtgaaatta ctgtgaatgg ttttccaggt atcgaaagtg
    10861 gcgaaaaacc attttatgaa gaaccacgta tgcctaaaga tctaatcaca agaactgatt
    10921 acgtaacggc taagaatgta ttagatgcga agggtgccga tgctttggtc gaatggatca
    10981 aaggacagga aaatttgttg ttgacggata cgacatttag agacgcccat caaagtctgc
    11041 tagcaactcg tgttcggaca aaggatttca agcagattgc acgtttgact ggagaaggtc
    11101 ttcctgaact cttttcaagt gagatgtggg ggggagcaac atttgatgtg gcttaccgct
    11161 ttttgaatga agatccttgg caacggttga gaaaaattcg ttctttaatg cctaatacgt
    11221 tgctgcaaat gcttttcaga ggttcaaatg ccgttgggta ttctaattat cctgacaatg
    11281 tattggtgga atttgtcaaa gaagcagcag cgcaaggaat cgatgtattt cgaatctttg
    11341 atagtttgaa ctggactcca caaatggaaa aaagtatcca agctgtacgg gatactggta
    11401 aaatcgcaga ggcagctatt tgctatactg gcgatatcaa cgatccaagt cgggcaaaat
    11461 acaatgttca gtattataaa gacatggcaa aggaactaga acaactgggt gcgcatatca
    11521 ttgcaatcaa agatatggct gggctgctta aaccgcaggc agcttatcgt ttgattagtg
    11581 aattgaaaga aacgacagat ctgccaatcc atctgcatac tcatgatacc agcgggaatg
    11641 gaatcatcac ttattctgct gcttcaaaag caggagtaga catcgttgac gtggcaatga
    11701 gtgcaatgag tgggaatact agtcagccta gcatgagcag cttgtattat gcactggtca
    11761 atggtccacg gctgccagaa atcacgattg aaaatgcaca gaaattaaac cattattggg
    11821 aagatgtgcg catgtattac aaaccgtttg aaaacggctt gaatgctcct gaaacagaag
    11881 tatatatgca tgaaatgcca ggaggacagt attcgaatct acagcaacag gcaaaagctg
    11941 tcggattagg tcatcgctgg gatgaaatca aacaaatgta tcatactgtc aacttgatgt
    12001 ttggcgatat cgttaaagta actccatcat ctaaagtagt tggagatatg gcactattta
    12061 tggtccaaaa tgatcttaca gaagaagata tttatgaaaa aggcgaaaca ctaagtttcc
    12121 ctgaatctgt tgtgactttc ttccaaggag aactagggca acccgttggc ggatttccta
    12181 aaaaattgca aaagattatt cttaaaggca gacctgcatt gaatgaacgt ccaggtcttt
    12241 tggctgaatc agtcgacttc aacgaagtaa aaaaagaatt ggcagaaaaa attggctatg
    12301 aaccaaaaca agacgaagta ttaagctatt tgatgtatcc gcaagtcttt ttggactatc
    12361 aaaaagcata taatcaattt ggcgatgtta cattactgga cacaccaaca tttttccaag
    12421 gtatacgttt gggtgaaaca atcaatgtac aaatcgaacg cggaaaaacg cttatcattc
    12481 gtttagatga aatcggagaa cccgatatcg aagggaatcg cgtactgttc ttcaatttga
    12541 atggacaaag acgtgaaatc actatcaaag ataattcgat catcagtacg gtccaaaccc
    12601 gattgaaagc tgaaccgaca aaccgagaac aaattggagc aacgatgtct ggttctgttt
    12661 tggatgtatt agtgaaaaaa ggcgataacg ttaaaaaagg tgatacgttg atgattactg
    12721 aagcgatgaa aatggaaacg gccatcgaag cacgttttga cggagaagtc gcacatgtat
    12781 atgtgtcttc aggagacact atttcgtctg gtgacctttt gattgaagtg actgagaaat
    12841 aataagcgaa aggtggggaa acatgaaaag actgggtttg ttttggctga ttctgctatt
    12901 agttattgtg gcattctaca tggaaccagt cttgtttccg ccaaaacttg aagtgaagga
    12961 aaactctgat caacaaatat caaaccaaat gacaacgtgg aaataccagg agcttaaagc
    13021 aactggtttt tctacttata tcggaaaacc gatcgatcag ctggaaaagg attttggcca
    13081 agcctatgat cgattaacta gcggatttgg ttttgaaaca agatattatg aaaataaagc
    13141 tagtcatctc acatttgaag cgaacatcca agacagcaga gtcacaactg ttaaagtact
    13201 gcaaacaggg cctgctgata tcgcgccgtt ttatttaggg atgacgatgc aagatcttac
    13261 gaaaatcact actatctata ctaatttcac ttttgagtac catgatacag aagttggaat
    13321 cgaactaatg gaagaggata tgaattatcg tccgctaatt gcttttgata acgacacttt
    13381 tgctatttta ttttttgatc aaacaacaaa cggcctattt tcagttgtgt atttagataa
    13441 agatagtctt ttgaaactat tgccatatca agtgtttggt gaaggccttc cacattatca
    13501 agcagataaa tcggctgatt gggaatcaat caatcgcgtt aaagaacgaa aaagtacgac
    13561 tttattaaat atgcttagaa gagaagacga acttcccata tacaatcaaa cacttgcctt
    13621 tcaaaaccac agtcagctgc tactacatga ttttttgaca aggcctgagg aaatattgtc
    13681 agaggaacgt atggctgatt ggcaaaaatc gctaggcagt gctcaaacga cagtgaaatt
    13741 ctcactgacg aatgatgaat tggagaagtt aatcagtaaa caaaaattag aaaatactag
    13801 tgggctattt attcatccgg cgattgacgc tactttttcg tttttatatt ggtacagcga
    13861 tccttacaac catgaccgtt tcatggaccc gggaacggat tctgtaggca ttgctttttc
    13921 taaagaaaat atgttagtct tattgcaaga agaaaacgga gaaagtagtg atagcagtga
    13981 tcgttaacga agagctgttt caactggagg atcaatgcct ccaagtcgct gaaatgataa
    14041 aaaagagtaa aacgatggct tcttatcttg aacataaaca gtcaatggaa gaatctgcaa
    14101 aagtttgcca gttgaaagaa gcgttcttgt ctaagaaaga agcatttgaa aatgtagcgg
    14161 catatggtga gtatgcacct gattatcgtg aaaaaaaacg agctgttcga aaagcaaaaa
    14221 gacagcttga tatggatgag catgttgcag ctttccgata tagtgaaaca cagctgcaaa
    14281 gtctgttgga tcaaattgtc caagtacttg ccgaagaagt ttctaatgaa atcaaagtag
    14341 atgcaggtag cccgtttttt gaaacgaaaa aacactttgg atgcggaggt aattgtcatg
    14401 caattagaca atagtgaatt tgaaataaca aaacgacgcg ggctgatcgt atgggtctac
    14461 agcttaaaac aattaaagaa tctgaaaaag ttcggcttgt tgcattatgt ctcaagaaga
    14521 atgaaatatg tcgtgcttta cttaaatgaa gagacattgg aagaaacaga agaacgaatc
    14581 cgtaaattac attttgttcg aaacgtagaa cgttcttacc gaccggatat cgaaatgaat
    14641 tttgccgaaa aaataggaaa caaaacagta ttaaaagacg aaggtttcga aattgaagag
    14701 ctaaatacag aaatccgatt agctgaacat ctttaaaaat ctcttatgag cagtttttaa
    14761 ataaaaaata acttggataa cgatcgattt ttgatcattg tccaagtatt tttttggtat
    14821 actagtacca gtaccatttc taaggagtga gtagatgcgg ataatatcag gaaattatgg
    14881 cgggcgccgt ttaaaaagtt tagcaggggc gaacaccaga ccaactacag ataaagtaaa
    14941 agaatccata ttcaatatga ttggaccata ttttgatgga gagacagtat tggatctgtt
    15001 tgcaggaagc ggtggtttag ctatcgaagc agtatctaga ggctgcagtc atgcggtatg
    15061 cgtagacaaa aattaccaag cgctaaaaat catcaaagaa aacatcgaaa ttacgaaaga
    15121 accagagaaa tttaccacaa taaaacttga tgcggacaaa gcaatcgaac ggctagcagc
    15181 ggagaaacaa caattcgact acttgtttct tgatccgcca tatgcaaaac aaaaaataat
    15241 cgatcaaatc cataaaatgg aagaactggg acttttttct aaaaccgcag ttatcgtatg
    15301 cgaaacggat aaaacagtcg aattgccaga aactgtggct ggattccatc aaataagaaa
    15361 acagatttat ggcataacgg ccgtaactat ctatagaaaa gagggaaatc aatgaaacgt
    15421 gcattattcc caggaagttt tgatcctttt acaaaaggtc acttagatac ggtggaacgt
    15481 gcggctaaat tattcgatga ggtagtaatt ggtgttttta tcaatacttc aaagaaaagt
    15541 ttgtttcccc cagaagaaag aatgacattg atcacaaaag ctgtttctca tcttcctaat
    15601 gtaaaagtca tgcatcaaga aaatcaattg acggtagaaa cagcaaaaga gattggtgca
    15661 gatgcgttga tcagaggcat acgaagtatc aaagactttg aatatgaacg agagattgct
    15721 cagatgaatc atcatctata tgatgaattg gaaactgtct tcttattagc aaaaccagaa
    15781 tatagtcacg tgagttccag tattttaaaa gaagtactgc actttggcgg agatgtgagc
    15841 agctacttac cgccagtgat caacgaagct ttagctggaa agcgtgaatc aaatgaaagc
    15901 taaacaatgg ttgaaaacag tactgattat catatttgga atgttgatca ttggatttgt
    15961 agtagttccc ttgccgtatt atgtagaatc accgggagcc acgatcgatc tgaaagaact
    16021 aattacggtc aatgataagg aagataaagg ttctgggtct ttctctctta cctctgttgg
    16081 tatcaggcaa gctacaggtt tcttagcttt aaaagctaaa atgacccctt ttgaagaatt
    16141 gatcagtgaa gaagaattga ctggtggggc gacaaatgaa gaatataatc aaatgcaaca
    16201 gtattatatg gattcttccc aaaatgcagc aatcgaacaa gcgttaaaac ttgcagacaa
    16261 gccttataaa atggaattca aaggcgtata tgtcatgagt gtagagaaaa catcgaattt
    16321 ctttggcaag ctttcagttg gagatacagt aacaaaagta gacgggaaaa gctttcaaag
    16381 tacagaagca tttatggatt atgtaaaaag ccaaaaagtt ggacaaacaa tcgaaatcag
    16441 ttatttgcat aatggtgaag aaaaaacagc ttctggtgat ctgatcgagt taccaacaga
    16501 taaaaaggca ggaataggta tcacgcttac tgaccatacc gagatcgaat cagatacaga
    16561 tgttcgtatt aattctggct ctatcggtgg gccatcagcg ggattgatgt tcactttaga
    16621 aatctatgaa caagtcactg gtaaaaactt gcgacatggt aagcaaattg caggaactgg
    16681 aacgatcaac agtgaaggag aagttggccg aatcggagga atcgataaaa aagtggcaag
    16741 tgcggataaa gctggcgtag agattttctt tgcaccagat gatacgattt cagacgatgt
    16801 taagaaagaa tatcctgaaa taaaaaccaa ctacgaagaa gcaaaagctg ccgctaaaaa
    16861 attgaattcc tcaatgaaaa tcgtaccagt aaaaacagta caagaagcgc ttgactatct
    16921 agagaaaatg aaatgaaact aaataattaa agaggggttg tgacacgagt tctgtcacaa
    16981 cccctctttt tcgtcaaaaa tcttcacgat aaaaataaaa acaaaagaaa aaattgacgt
    17041 aattagatgt tgaagggacg attttgccag agctcactgt ttcttcaatt ttaaatgaaa
    17101 ggagtaatga atgattttta ctggtcattc tttgaaaaaa gaacaatgat agaaaaaatc
    17161 agcaagaaaa tactagcttc acccaaattg atcatttgga tgtctatgat agttcttatc
    17221 ttttgtatcg caagtggttt tttattatca aatttgctat tttatagaga aacagaagag
    17281 actgctgcaa aagaaatact ttcttcgttt agttccagtg gagaagatcc atcagctgaa
    17341 ggaaacgatg tagaagagga tgctcgtcca aaggtcatgt acaccgatat caaaggaagt
    17401 gtgaaggagc ctggtatcta ttccttttct tcagaagaac gggtctatga tgtactaaag
    17461 cgtgcaggtg gattattaga agaagcggat agcgatcgaa tcaacttttc tgcgaagata
    17521 gaagatcagc aagtcctata tatccctgct gtaggtgaag agctgccaga acatcttgat
    17581 cagttggcat cctcggaagg aaaacagcct acagcagatt ccgagcctcc caaaataaat
    17641 atcaatacag ccactccatc agaacttcag cagattcctg ggatcggatc agtcaaagcg
    17701 caagaaatca ttcgatttag agaagaaaac ggatccttcc agaaagtgga agacctgcag
    17761 gagatttctg gtatcggtga aaagacagtt gaaaaactaa aaaacttcgt tacaattaaa
    17821 tagaatgatt caaaatcgac tggcacgagc aataaatgga cgctaatgga tactaagaat
    17881 aatccaattc taagagcttg gcaggacgag gaggtagtat ttttggatag gaaatagctt
    17941 gattgttcgc aaagaaacat taaaaaaacc aatgctgaat gtctatctcc aaaataaaat
    18001 atctgggatt catattatga atacggctgt cagcggcaat aacagtcaag ctctacgaga
    18061 acgctttgcc aaagatgtac ttagctatac agcagataaa gtatttattc tcattgggac
    18121 gaatgatcta gcagagcata aacaactttc taaagaaacg taccaaaaaa tctgcagtgg
    18181 atgatagttc agctgaaaaa agactatagt tctgatcatg ctttctttat cacgccacca
    18241 gcagtcgatg agacaaaaca aaaatatcgg aataacaagt tgatcaaaga atatatctct
    18301 gttctcacag aagtagcaaa acgccatgat tgtgaagtat cgatttttat acggaatttc
    18361 ttgaacaaat ggattcgaag aatttttata atgggctaat ggatgacgga cttcatttcg
    18421 gcagtgaagg gtaggatctt ttgtcagaca ggatgattcg ttatatttaa agaatatttt
    18481 gtttaagcaa tgagtacttt ttaatcaact tgaaaaaacg gaggaatata aaacatgtta
    18541 gaaaaatctt caaaagcatg cgatacgaat aaagaaaaag ttactgataa gtcacataat
    18601 gaacgcattc cttgggacca gtactttatg gctcaggcag tactgttatc tttacgtagt
    18661 acgtgtacac gtttagaagt aggagcgaca ctcgttcgag aaaaaaggat cattgctggc
    18721 ggctataatg gagcagtcag tggagatgtc cactgcatag atgaagggtg ttacgtcgtg
    18781 gatggacact gcttgcgaac gatccatgca gagatgaatg cactcctaca gtgtgcaaaa
    18841 cttggtatcc cgacggaagg ttcagaaatc tatgtcacac attttccatg tttggcctgt
    18901 acaaaggctt tgttgcaagc tggtgtgaaa gggatcaatt atctccatga ctatcgaaat
    18961 gatccttatg cccaagcgtt gatcgaacaa ttaggtgtat ctgtacacca agttgcttta
    19021 gactcacagc atttttcaaa acttcaaaat gaattaacag gaaaacatgt ctagcgtttt
    19081 agaaaggcat gttcgaaatc ggttgatttt tccggcaatt acgtcgatga ttatctgttt
    19141 gttgtattac actgacaatt ggggttatcg tttgcttctt gccggaattt ttttatggtt
    19201 ttcgctaaaa gaaggacgtt ttatcgcgta cttgtgtatt ttgtgtagca taacggtatc
    19261 catttcttat aggctacaag agtattcatt tttagaggaa aaaacaacat ctgcgcaaat
    19321 tactgtattt acagatacga ttcgtgcaaa tggtaatttt ttgacaatgg aaggaaggtt
    19381 atcagagaat aaacaaaagc tccagctttc ttatcaagta aacacagaag cagaattgaa
    19441 aacatggctt aacttccgag gaaagggaat agaattgact gctgaaggcg agttcatctc
    19501 tccagcaaaa aagcggaatc tttataattt tgaccaagaa gcttacttca ggaaccatag
    19561 aatttctgga cggttccaaa ttcaaaagat tgaacagatc accatgcaaa atcactggcg
    19621 aaactggctt cagagaaaaa gaggatcttt tatctccttt gtccaatctg aatttccgca
    19681 aaaaacaagt gtatatatta attctttatt atggggctat aaagatgcag gattcagaga
    19741 accagaagaa atttttaggg agagtggttt gctgcatctg ttcagtctat cgggtctaca
    19801 catccaattt tatttagggt ggctgtatta tttgtttcga cgtatcggat ggccactaca
    19861 cgtcagtatt attccgttga gtctactaac tatctgttat gtttctattg caggaagttc
    19921 tatttctgtt ttgcgtgcta gtttgctgtt tttattgcga ttgctagtca aattgctgaa
    19981 taagcattat tccaccatgg acttatttgc agttgtcttt tggcttcttt tactatttga
    20041 tcctcgattt ctttttcatg cgggtggaca gcttagtttt ttcatgagtt ttttgtttat
    20101 cctgatttct tttgatggag attggttgaa aaaaacggcc tctcacgtcc tgtttactat
    20161 gatcacgatg ccgttgattg tatggcattt ttatgagtgg cctattttag gtagtctttt
    20221 tacattactg attgcaccgg tattcaagtt tctttttctt cctggtgtgt tatttctcac
    20281 actcttcaat cgctggctgc cggaaacttt acttttgctg atagaagagg gattaatctt
    20341 atttgagcga ataatcgatt tttcttcagg gtcgtctctc attataggac gtttgcccct
    20401 tgtgctatct gggatgtata ttttcggatt actgatttgt atggataggt tgaaagaaca
    20461 acctgtgaaa cacttgtttt atgttggtct tttttctttt atttttttga ctttgccatt
    20521 tttacgcttt tcttcaacga tcgcttttat cgatgtcggt caaggagaca gtatcttttt
    20581 gcaaagtcct tttcgaaaag aaacgatttt gatcgatact ggaggtcagt tacactttga
    20641 aagagaaaag tgggcgcaag gtatgatacg tcctcctgct gaaagaaata tcgttccata
    20701 tctaaaaggt caggggataa gtgtaatcga taaactcatc ttgacacacg acgatacgga
    20761 tcatgtggga gaattaccca cgttatctca gcattttact atcaaaaaaa tttatatcgg
    20821 ttggggcgct ggacgaaatg agccattaca aaggcattta tctgaagctc aaaaaaatgg
    20881 tactgagatc attgaaatca aacatgggga caggatcagc ggttattatg atctttatgt
    20941 attgactcct tttgaaaagg gagagggaag aaatgaagat tcgattggtc tatggatgga
    21001 atataacaat aggcgctttt tatttttagg agatttgaat caagcaatgg aaaaacaatt
    21061 gcttttgatt tatcctaatc tgaaagccga tgtagtcaaa ttaggtcatc acggtagccg
    21121 tacgtcaagt aacacagatt ttctttccca tattgaagca aaacatggga ttatatcctg
    21181 cggcgtaaat aatcgttatg gtcacccgca cagcgaagta ttggagacac tagaagaaaa
    21241 tcagataaaa acatggcgaa cggatcaaca aggaatgatc acctatcgct ggcatccagt
    21301 ttttcatcct catgggctag tggaaacaat gatagactag taattctcat ctgctcatgg
    21361 taggataatt tttgaaagtg gtgagtaatt tgagtataca agcagagtta caaaaaatac
    21421 gtaaagaaaa acttgcatct tgttacttga ttttaggaac agaaaagttt ctccaagatc
    21481 aagttcggac agaaattgtc aaaaaaatcc aattagaggg cgaagatgac ttaaattttt
    21541 taagttttga catggaaaat gcaagtttag atgaggtagt agctgaagca gagacgttgc
    21601 cgtttttcgg agatcaacgg ttagtattcg tagagaatcc ttatttttta acaggtgaaa
    21661 aagtaaacaa tggaatcgaa caaaatactg atttattgac ggcttatttg aaagaaccat
    21721 tagaatcgac tgtattagta ttctttgcgc catatgaaaa gttggatgaa cggaaaaaag
    21781 taacgaaaca attgaaaaaa acagcaatta tcatcaatgt ccaacagttg aatgaaaaag
    21841 aagtacgtca atatctgatg aataccttag agaattcctc aatcaaaatg gaacgaagtg
    21901 caattgattt attcttgagg ttgaccgact tagatttatc caaaatgatg agagagttac
    21961 agaaattggt cctttatgga caaaatcaac agcaaatcac ggtaagagaa gtagaagaat
    22021 tagttcctaa aacgttagaa cataatattt ttgacatgac ccaatatatt ttaaaaggga
    22081 agacggaaca agctttgcgt ttatacgaag atttagttat gcaaggggaa gaaacaatca
    22141 aaattaatgc gattttgctt tctcaattac gtttattttt acagacaaaa tttcttgtaa
    22201 agattggtta tcagcaggca aatattgctg aaacattgaa aatccatcca tatcgtgtaa
    22261 aacttgccat gcaggaagta aaaaaattcg atgaacattt gctggtacag ttgtttgatc
    22321 aattagttga gatggattat caaatcaaaa cgggacaaat cgaaaaagaa ttaagttttc
    22381 aactattcgt tttgcgcaca gggcagttgt taagaaatcg ataagaagaa agacgagggc
    22441 aaagcatgcg ggaaggtcgt ttgtttttgt cctttttttg acaaataaaa aaatcggccg
    22501 aagccgattc ttttatttag cgattttttt gcttaaacga gatttgtcgc ggttagcttt
    22561 gtttttgtgg attagacctt ttgattcagc catatcaata gctttaacag cttctttata
    22621 taacgcatct acgttgtcag cacctgaagc tacagcatct tcaaattttt tgattgcagt
    22681 acgcatagcg cttgtttgag atgaattttt cacattagcg ttttcgcttg tacgaacacg
    22741 tttgattgca gattcaatgt ttggcattag tttcacctcc gataacttaa gttcttacgc
    22801 gtgttaccgc ataattcaac attcattatt atacctaaag gcagcgtgct ttgcaatatg
    22861 aacatgacaa gtttttttcc ttaacagtct tttattagga ttttttttca gtaaaaacgg
    22921 atagtacaag ctgaaaaaaa tcaaaaagac gctattgtgg gaggcaatag cgtcaaacca
    22981 aactagattt ttatttatct atttaaacaa agtttgaatg ggaatgtttg gtactaagtt
    23041 attatataac aaagagaacc gtttgcaaag ttttttttga aaagggatac gattccctgt
    23101 tgttctttgt ttttctatta agaaactgca gttattttat aaccagtatc ctttgatcag
    23161 taaaggctat tctggtgtac cgattatttt aatgtattca tcgatatttt ttatcaacat
    23221 tttttttatg accaactggt tccgacggtt ccatttgcca cttacttcca gcacatattt
    23281 atttaggggc aaacgtaaga tatattttgc caaatagcga tctttaagca ggccattgat
    23341 cacacggtcc tcaagctgca agttgatccc agtcagcatc attggctgtt cttgtaaaac
    23401 gtacgtatca attaaaagac cttcaaaagt ttccacaagc gtcttccttt cttttcaaag
    23461 taacctgaaa acaggcacta attcttccat tttgatattc gttgaagtgg atgcgatcat
    23521 tctttctttt gtccactgta atctagtata acaaaaaaga gcagaagagc caaaaagaaa
    23581 aaagagcatc gaattctata ggaagtctca ttggcacggc ctaaattatt acaaaacatt
    23641 gattaaaaaa agcccctgca tcgcaggagc atcccctata tcgttttaat aggtcaatca
    23701 aaaagcagta tcaaacacat tttgtatcaa atcaggaaat aatagataag ctaagcccag
    23761 aatcaaaagt accgctagaa gaaaccagcc gattgttcct atgaaatact ttaatttttc
    23821 gaacattgta tcacctcgtt gtcagtatat cacggatcag taaattgggg gtgttaagaa
    23881 tctcctaaaa tagacaaaat ttgttctatt ttcctaaagc aaatacttgt aagatacagt
    23941 aagtgatacc agaagcaatg accggtccgg cggcgattcc tcttaagaag acaacaccta
    24001 aaatcgtgcc gaaaacaagt gcaacagtga tttgtggatc agaagcaatc aatcctacac
    24061 ccttggaaga taagacagcc actagcccac cgcaaaaaac agctacccag ccaatagggg
    24121 atttaaaggc ttctaataga tcacgtagac cgatttctcc tgttgcgata gggattagaa
    24181 tagccgctgt gatgatcgtc accccaatat tgattccttt gccttgaagc caagggaaaa
    24241 aggaacggga ggctggtatg agcttcataa tcaaaacaaa agctgccgct atcatcagac
    24301 tttgattttt agctaaaatt gctacaagta aaataattcc taaaaaaagc caactttcca
    24361 tgaaatatat tcctcacttt cttcgaaaca atcataacat gtttttagaa agttacaaaa
    24421 gtaggaattc atccttcaag acctattcat aagctgtttt tttcataaaa taaacaaaat
    24481 cataaaaaaa cactttgctt atttgacaga agacagccgc gctttgtata gtggacaaga
    24541 cttacatttt tttgaagaat cagaaagatc aggtaaaggt cttagtaaac gcaagccatt
    24601 cagaataacc aatattgtgc ttccttcgtg tcctacaact ccaagaggta gatttaaaaa
    24661 ttgcaggaag ttagaagcaa tcaacaagag aataacaaga atcgaaaaaa taatattttg
    24721 tttgatgatc cgccgcattt ttaaagatag ccgatgactg tagacaaatt tatttaagtc
    24781 gcttttcatt aagacgatgt ctgcgatatc catagcggca tctgtccctt gccccatagc
    24841 gacacctaaa gaagcggctg ctaaagcagg cgcatcattg actccatcac caaccatcgc
    24901 atttacttgg taaagttcat tctctttttt gactagttgt gttttttctt ccggagtaca
    24961 gcttgcatag taatgttcaa tccctacttg ttcagcaatg gcctttgctg tgccagaatg
    25021 gtcgccggtt atcatacttg tatggatgtt ttgggattta aagtaggaaa ttacttgttg
    25081 ggtatttgct tttggaatat ctagcagacc taaaacagca attaactgct gatctttaga
    25141 aagataaatc acggttttgc cttgattttc caaacgttcg atcatttccg caatctcttt
    25201 gctgattatc atcgtctcag gatcatatgc gtgcttgcca actttccatt gcgcacctaa
    25261 ataggtagtt tgtaatccaa aaccagtaat ttcttctatt ggtaagtatt ggatttcttc
    25321 aggtattgtg caatcgaaat attgcaaaat cgcatgagca agtggatgcg tcgttttttg
    25381 ttccatagca acaagaagtt gttgtgccaa atccttttca tcaaagaaaa agctgtcagt
    25441 tacaactggt tttcctttag tgagcgttcc tgttttatca aaggcaatcg cttttaattc
    25501 agaaagcttt tctaaatgga taccgccttt gattaaaatc ccattttttg cagaatttga
    25561 aagagcagct agagaagctg gtgtagcaga tgcgacaagc gcacaaggag aagccacaac
    25621 taataatacc attcctcgat aaaagctttc atcccaactc caacctagga aaaagtgggg
    25681 gagaaggatc attagaggaa cagcaagcaa aatcaatttt acataattat tttcgattcg
    25741 ttcgataaat gaagctgttt gagttggcgt attctgcgct tcttctacta attggataat
    25801 cttagcaaag acagtttcgg aactcgtttt tgttactcga atcgtgatgg gatttccttg
    25861 attcagcgtt cctgcataca tctcatcgcc taaatatttt tctacaggca cagattcacc
    25921 gcttaatgca gactcatcga tcgttgcctg tgtgctttct aaaaagccat ctatcggaac
    25981 agctgctcct ttagcgacaa ataccaagtt atccacgctc cttcaaagta attaccgatc
    26041 aaacaggcac caatagcagc taaagccatc aataaatcca cattcagtgt ccgatcattc
    26101 cacaattctt tcaagccttc cgatgtttgt ttatatccac cgatgataat ggcactgata
    26161 taaaaaagtg gggtgatggt aagagagaat cggtcgaaga taaaaccgaa gatcataaat
    26221 aaaaggcagc cgattgttgc tgcaaaagct tttttctgat tagtcatcgt tattagacct
    26281 ccttgtcaaa acaaggatag cataataaaa atgtaattga taataataac agctaaatga
    26341 gaacgataat ggttatcatt tggtgattaa gcttgatgtt gtttttatta tttataatga
    26401 ttattattaa agaaaataaa aaaccagaac atttctcaaa taagagaagt gttctggttt
    26461 tttctacatt gcaataaacc aataaccaat caaaatgata ccaaggataa tacgatacca
    26521 gccaaaagcg gtaaagtcgt ttttcttcaa atagcttagt aagaatttga tagctaagac
    26581 ggaaaccaca aaagatacga atgttcctac aagaagaaca acagtttctt ccattttgaa
    26641 tgaattccct tcagctaaat atttgacgat cttcaagccg cttgcgccaa acatgacagg
    26701 aatccctaag aaaaatgaaa actctgtagc gacaaaacgc gaagcaccaa tcaagataga
    26761 tcctaggatc gttgcgccag aacgggatgt tccgggaaca agtgccaaga cttggaataa
    26821 cccaacaaga agagctgctt ggtacgtaaa gtcattcaga gaagtccaac gaggtgtttt
    26881 gtttttgttg cgtttttcga caataataaa ggcaataccg taaacgatca aaacaatggc
    26941 aactggtaag aacttgtgga aatttgcttc taaccaatca tccaaaggca aaccaatgat
    27001 gatggctggt atgcaagcaa cgatcacttt aaaccaaagt gtccacgtat cttttttttc
    27061 taccgttgat ttacttggag aaaatgggtt taacttgtga aaataaagaa cgacgacagc
    27121 caagatcgcc cctaattgga taacgacatt gaacatcgac ataaattgtg aactggcatc
    27181 cagtttgatg aattcatctg ctaggatcaa atgaccagta ctactgatgg gcagccattc
    27241 cgttattcct tcaataatac cgagaatcac tgctttgata atatttgcaa aaaacataaa
    27301 ttccttcctt cccaattaag atacatctta tagtataccg ttattttttg agaaacccaa
    27361 tctgtttttt aatattaaga aaaacttgag aaataaagca gataagtaca cgaagagaaa
    27421 aaagaagaca aagccctcgt tcagactttg ccttttatga ttctctggta aaaattaaaa
    27481 tatccccatt atagaaacga gtcgcgacta tgtgttttcc cgttatcgat ataagacgta
    27541 tcattgatgc cgtggataga aaatcgaccg tttgcataag tgacctttgt tacgctgcca
    27601 tttaaaagcc cagcacgaac aggctgttta ggatcgatca gattaagcaa aaaggcgatc
    27661 gtcaaaccat gagatacgac taaaacattt ccaccaccat ttttttcgca gtgatgggcg
    27721 atatcttcaa atcctgtcca tacacggtct cgcaatgttt cataagtttc cgcccaccca
    27781 gcggtatcta agttgtatac agcattagca atttgttcaa aagaaacttc tgtggtaaac
    27841 atttcatcat atgttttaaa attcaataca cgagggatca caccccacat ttccatgtca
    27901 tatccacctt caagagaccc gaaacaccat tctctaatcc ttttttcaat aaaataagga
    27961 atttcttttc ccttagaatg ttctcctaga ataatacggg cagtttcgat tgcacgacca
    28021 ctgtcactag aataagcaaa tgaaaagtcg acatttttta acccgcgacc taaataatga
    28081 atgccttctt gtccttgctt tgtgaggggt gtatcacacc agccttgtac tcgttcaatt
    28141 gtattgaaca tcgtttttcc gtgtcgtacg atataaagtg tagtttctgt cattttattc
    28201 tcctcctaag tcgttttaac gattgaaaaa aggatttgtc cttttttcct gttcaatcgt
    28261 cgttgcatcg ccatgtcctg gatagactgc aaattcatca ggtaatacaa acagctgggt
    28321 ccgaatacta tgaagtaatt gttctaaatt ccctgtatac agatcggttc taccgatact
    28381 tcctctaaac agggcgtcgc cagaaacgac aaaatcatca aaaataaagc tgacgcttcc
    28441 tatagaatgc cctggggtag ggacgacgcg aaaagacatg tttcctaaag tataagtttt
    28501 catttcaaat tcatattctg caggcgagac aataacatca gcaatatcat catgtctacc
    28561 gagtccagaa agattcaaaa tcggatcacc tagccatttt tgctctagtg ggctgacata
    28621 aactgggatt tgataatacg cgcgtaaagg ttccactgca ccgatatgat cgtagtgagt
    28681 atgggtaagc aaaatcgcta ccggctgttg gtttgttttt ttgatttctt gtttgattcg
    28741 ttccgcatct tctccagggt caatgatcaa aagtgcttct tcgttgtaga ccaaataaca
    28801 gttttcttca atcattcctg tttttaatct ttcgatatgc atgtgcattc ctccttgata
    28861 gccaaaactt ctttcatcca ttagtataac gcattcattg ctaggaaaca aaaacaggta
    28921 cttatttctc tttacttatc tgaattatca gactatatga aaagtatttg gatattctaa
    28981 agagaaggaa agtaagtttt catagcattt taatatgtcc ttgttacatt agattcatcc
    29041 aaaaatccaa attctatatc ctatatccct gcctaatcag cagggtgttt ttttatgttt
    29101 ggaaagagag ctctttttgc taaactaggt tttttcaggt aatcttagag agaaagaggt
    29161 ggtggaaatg atcaaacagt caaaaatatc tgtgcggcac ttagtagaat ttattttgcg
    29221 acgtggaagt atcgacaatc gtaaaaaaag taaccatact gcattagaag gagcgagaat
    29281 ccaccgcaag ctccaaaaag atgcgggaga aaactatgaa aaagaagtct ttttaaaaac
    29341 aacagttcaa ttagatgaat attacctgat cgttgaaggc cgtgcagacg gtattttcaa
    29401 aagagacggt atttattata tagatgaaat caaaacctcc gaaccacgat ttgaagattt
    29461 agaaccagaa caagtggaac ttttctttca tcaagcaagg gtgtatgcct atatctatag
    29521 tcaagaacat gatttacaag aaataaatct gcaattgact tacttccaga cggcagaaga
    29581 agtgatcacg agaaaggttg aacatcagac aagagagcag ttagaaactt tttttaaaaa
    29641 gctgactgaa gattataaag aatggcttgt ttttcaagaa aactggcgca ctgtacgcaa
    29701 tgcctctttg atggctttga aattccccca tgatgagtat aggaaaggtc agcgtgaact
    29761 agcggtagct gcttataaaa cgatccgtac gaaacagaaa ttgtttgtag aagcacctac
    29821 cggaacagga aaaacaatct ctacgttatt ccccgctttg aaagctgtag gggaagaaga
    29881 gggagaaaag atcttttatc taacggctaa aacaataact agacaagtag ctgaagatgc
    29941 tatgacagca ttaaaagata caggagctga agtaaaaagt gtcactttga cggcgaaaga
    30001 caagatctgc tttttagatg agacgacctg taatccagat cagtgcccat atgccaatgg
    30061 ttattacaat cggattaatg aaggtttatg ggatcttttg aatcatgaga atcagatcac
    30121 tcgtgaagtc atcgaaactt atgcaagaaa gcatacactt tgtcctttcg aactatcatt
    30181 agatgtcagt atctggtgtg acgtaatcat tggggattac aattatttat ttgatccaac
    30241 tgtttattta agacgttttt ttgaagatga aaaaaacgag gactatctgt ttttgattga
    30301 cgaagcacat aatttggtga atcgttcccg agagatgtat tcggcagaac tatcttatga
    30361 aaaaacgaaa cgaagtaagg aagctatacc aaaggaattc aaaaaacttc atcgccgttt
    30421 caacaaacta ttaaaagaat ttgattcgat ccgagaaata gcaaaagaag atcattggga
    30481 ttatcatcat caaaaagcac cggctgaatc tttagtaaag gcaggttatc aattaagcga
    30541 aaaaattaaa gaatggctgg cagaatttcc tgagcaccca tcacaagaac aattgcttcc
    30601 ttattatttt gatttgcttc attttttgaa ggtcagtgaa ttttatgatg atcactacga
    30661 aacaacagtc gaaaaaacgt atcgcgatct gatagtaaaa gagttttgta ttgatccaag
    30721 cttgtttctt gagcagtcgt tggataaagg aaaaagcagt attctttttt ctgctagttt
    30781 ttcaccactc tcttattatc aggaaacact ggggggacaa aaaagtctgg cttacaaact
    30841 gccgagtcct ttcccagaag gaaaccagca agttttcatc gcaaattacc tagagacgac
    30901 ttatcgaaaa agggaacaca gtctggcaaa attagtagag acgattcatg cttttgctga
    30961 tggaaaggtc gggaattatt tcgttttctt tccttcttat caatatttag atcttgcggt
    31021 ggaagcattc agacagcaat atcccgacag caatgtgcta atccaagaaa cggacatgaa
    31081 tgaggaagaa cgcgaacgat ttttaacaaa atttcaaaca aatcccgctg aaacattgat
    31141 tggattttgc gtacttggcg ggattttttc agaaggaatc gatttgagag gggaccgatt
    31201 gatcggcagt atgatcgtag gtgtaggttt gccgcagatg aatcatgagc aggaactgat
    31261 caaatcatat tatgatgaga aggaacacct tggatttgct tacgcttatc agcttcctgg
    31321 aatgaacaaa gtcttgcaag ctggtggtcg agtgattcgt ggcatggcgg atcaaggaat
    31381 cgttgtattg gcagatcaac gattttctag tttttcttat cgacgtttat tcccacaaca
    31441 ttggcaaaca gcaagagaag tacgaagtgt tggggaattg gaagaaggaa tacagcgttt
    31501 ttggttatca aaagaagcag taaatcaaaa aaacgaggaa tgacagacgt cagcctcgtt
    31561 ttttgattaa cgatagttag tgaattgcaa ctcgattggt aagtcaagtt tacgtaataa
    31621 agcaatcaca gcttgtagat catcacggtt cttacctgtc actctaatct gatcttcttg
    31681 gatttgtgct tttactttca gtttttcatt tttgatagct gtagtgattt tcttcgcgtt
    31741 ttcacgatcg attccgttga ccaaatcggc agtttgtcga gctttgccac ctagagcgtg
    31801 ttctgattcg gaaaaatgga tatttttgat tggcacattt cttttgatca gcttaccaaa
    31861 taatacatct ttgatctgtt cgattttgaa atcatcatcg ctcaatacaa ctagattgct
    31921 gttctctaat ttgatttcta ctgtagaatc cttgaagtca aagcgattgg acaattcttt
    31981 tgttgtttgc tgaatcgcat tttttacttc ttctgtattg acttctgata caatgtcgaa
    32041 gctagcatct tttgccatag gaaccctcct gatatacata taaatattta ccatacatct
    32101 atcttatcat aggattaggg cagattctta cctgtttgct aaaattcttc tcatttgagt
    32161 tcgaagtatt ttattttaat agataaactg ttattcttac atttggaata ccaaagtagg
    32221 ggagaggtgc ttgtgagtaa aattatgact gcgtatcata aaaatgaaag attaaagaaa
    32281 atagtggtta ttttgataac cggattgacc gctgcggtcg ctttaaatta ttttttgatt
    32341 ccagccaatg tcttttctgc agggatgaat gggatcgctc aaatcatttc ctcgctttta
    32401 tatgaatggt ttcatatcaa gatagacaca ggaacattta tttttctttt gaatatccct
    32461 gtgtttgtat tagggtttct caaagtagga aaacgagcaa cgatccttag ttttgtgaat
    32521 gttgtatgtg tatcggtgat gacgatgctg cttccagtcg gacagatcac tgataatatt
    32581 ttgatgaatg ccctagtagg cggtgtttta ttaggtcttg gcgtaggatt atctttaaaa
    32641 atgggcttta caactggtgg gatggatatc atctctcttg tactttcaca aacaactgga
    32701 aaaacagtcg gaaactacat gctgattcta aatggtatca ttgtagtcat cgcaggcttc
    32761 ttctttaact gggaaagtgc cttgtatacg attatttcca tttatgcaat gactcaagta
    32821 attgatgcca tccacacgag tcatcaaaaa gtaacagcaa tgatcgttac cgtcaagcca
    32881 gaagaggtaa cagaagaact agcaaaacgg atggtacgtg gaatgacatt actgccgtct
    32941 gtcggtggct attcaaaaaa acaagggaac atgatcatga tggtcatcac ccgctacgaa
    33001 atgtatgatc ttgatcagat cgttcatgaa atcgacgacg atgcatttat caatatttta
    33061 ccaactcatt cagtatttgg ccggtttgca aatgaaaacg aacaaaaaat gtatcgaaca
    33121 actggcgtat tgccagaaat aaaaaatcgt tcacaaaaaa aatgaggaat caggtttatt
    33181 ttagaataaa tttaatctat tacatctaat aagaacattt ttataaattt aaacgagaat
    33241 cttacttcta aattctcatt tacaagcagc taaaaaaaga gtatgataaa taaaaatcgt
    33301 gttaaggagt gtcgtacgat gagcgaagca aaccaagaac ggtctggaca agaaatagaa
    33361 ctaatcaaag aacgtattct tgctgccttg gaaatggtga ttgatcctga attaggaatc
    33421 gacatcgtca atttaggctt gatttatgat atagaattca atcctgaaaa tggagaaaca
    33481 gtcattaaga tgactctaac aactatgggc tgtccattag cagatatttt aacagaatct
    33541 atccatgacg cattaaaaga agtgccagaa gtaacgaaag cagaagtcaa actagtctgg
    33601 tatccagcat ggacgacaga taagatgagc cgttatgcac ggattgcgtt ggggatacga
    33661 tagaaatctt ggctgactag ctatgagaag caaataggaa aaacaaaaaa gccttttatc
    33721 ctttctagaa gaaaagagat aaaaggtttc tttgtttata aaattttgtc tgctatatta
    33781 taagttaatt aaataaatgt gttctattga taaaggacat tcaaatccct ttccttttgg
    33841 aatccttctt ttttacatca gtttgatttt cgtatagtat tctttctcat cttcacgata
    33901 aatcgcagcc ccattttttt gttgtgcttt aagactgcca agattttttt tatttacaga
    33961 caaaagtgcc tcatcaatgc cgattttttt tgcctcttgt agtactaatg ctaatccttg
    34021 tgtggcatat ccttttttac gataattagg ggagatgccg taaccaatat ggccagcgcc
    34081 gtttcttaat gtttcgttca gtttatggcg caaattaaaa attcctacat actcatgttc
    34141 atcactttct aaaataaaga cggttgcggg aacgtaactc tcaggaagat cgattcctaa
    34201 agacgttttt tcgcattttt ttacataatc taaaaattca gcaaatgtat agccataagc
    34261 attgttgata aatccactct catttggtcc aaagctagtt tgtagctgat aagctttctt
    34321 taaatctgct tcccatagtt tgatcagttt catcgtattc acctctttaa gtaaccttat
    34381 tcatttgttt tctactatag cactattgtt gtaaatagcc atgcctattt ttatttattg
    34441 tgtcatatcg tattaagaat catcgaacaa tacaaatatg aattacaata aaaaaattat
    34501 ttgaagttcg ttcattcgtt acttatgatc tctagaagaa agaaaacgtg aattacttca
    34561 gcgtctagaa ttccagaagg atagtaatca acagaagaaa taatcaatat ccaaatgaaa
    34621 gaagatagct gaatcgagaa aaagaggaaa aaagaaatga agaaaaaaat aaaatagtta
    34681 ctaatcccag agtcgttcgt ttaacattga aatcgatcat atggataaaa cagatttttt
    34741 caccaaccct caatagatga tggagttgct cggatgaatt atttgtgtag atggcataag
    34801 ggagggcgta cagaaatgaa aaagaacaaa aatatgttct tgacaaaaaa cacatgtaat
    34861 cctaaactaa aagatgtgat gcggctatgg cggaattggc agacgcgcta gcttcaggcg
    34921 ctagtgaggg tgacctcgtg gaagttcgag tcttcttagc cgcataattt gtttttttat
    34981 aaagagaagg tacacctgga gcgatgagaa agaaaataac atattatcta aaagaatata
    35041 ggaaaaattt taaacaacgg aaccagattc aaataaatca aaaaaaattt gtgtgaaggt
    35101 gggacagttt aaaacctaat agattgattc aaatagtaga tttatagatt ctgatacaag
    35161 aaatactggc aaacgatagt taaggaataa agactaaact taaactcaaa aagaggtcat
    35221 gcgaaagaat aaatcgcatg acctcttact atattttgaa tgtgtttaaa cgttcaaata
    35281 atgaatctta gtaccaaccg tttgcttccc agaatgcttt cgccgcttcc caagaaccat
    35341 aacgtgaagt aacatattgt tctgctactc tttcttggtt tgcagcagag tagtcaccat
    35401 ttaagtaaga agaatctaat tggtaacggc cgatgtaacg tccgttcgtt gctgtgtaag
    35461 aaccgcttga ttctttttgt gcgatccatt cttttgcaga gcttgtactt gctggagtag
    35521 cagcagtttc tgttacagca ggagcaggtt gtgtttcagc aacaggcgct gcttgtgttt
    35581 ccgtttgaac cgtttcttgt acaacaggtt gttcagcttg aacaggttca gcttggactg
    35641 gtgcttcttg aacggctgct tgaggttcag cagctgcttg tgcagagcct tctgttggga
    35701 ttgttaattg ttgtcctgag tagatcaggt taatatcaga aattgaattt gattctgcaa
    35761 tggcattgat caaggagtta tcaccaacgt atttttgaga aatcgttgat agagtatcac
    35821 ctgattgaac ggtataagct tcgtccgcat gagcagtcgt tcccatgaag aatgcagcgc
    35881 cggcagccaa agttgttcca aaaagtaaag ttttaagtga tgtcatgaaa aatctccttt
    35941 attcgttaaa aagttttatt tgttgtgttc aacgaggaat actttaacac gtgaaaatat
    36001 ttcataggta aaagaagcat gacaatttat gacaaatcca cgttttttgt aacagaatga
    36061 tgttcgctcg atgtgaaaaa tgatgaggaa tttcacataa tgcagaaata atgggattag
    36121 aacaggtgtt ctgatgagaa aagaaatgta taaataactt gaaagaaatt gcttttgttc
    36181 atcattttat ttacatattg cgtatatttt ttgtattttt gcttgagatg ttacaataaa
    36241 agaaaaagga ggcggttcca atgagtaaaa gataccagaa catcatggta gcagttgacg
    36301 gatcaagaca atcgatcaaa gcgttcgctg aagcccttga cttagcaaag gacaatgaag
    36361 cccacttatt tatcgtgtca atcatcaaca aagttgagct gacccatagt gcctatgcct
    36421 tttcaaaaat ctatgcagat gaaaaacaaa gaacagaagt agccatgctg aaaaaaatca
    36481 atgatgccaa agagtacggc attgctgaga tccacgcaat agttgaaact ggagatccaa
    36541 gaaatttgat tgccaatgtc atccctggac aagaaaatat tgatttgatc gttatgggag
    36601 cgacaggaaa aggtgctata caacaagcat tagttggatc gaccgcttct tatgtcgtat
    36661 cacatgcccc ttgcagtgtg ttggtcgtga agtaatgaga taagggagga atagaaaatg
    36721 gttcgtcctc aagaagtccg tgcgccaaaa gaaaaaatag aaattttagc aattattgaa
    36781 gacggcacac aaacaaagaa gggatactcg atcgctttag taaagtggaa aggaaaaaaa
    36841 ggtgttgctc tgagatggga tggagataac caacaagata aaggattccc tgtcacagct
    36901 aacggttatc atccagcgtg gtttgttctg cctgataaat ttatggaatt atacagctat
    36961 gattatgcaa gagccgcagc atcaatcaaa gcattagata aattaacaga agagcagaat
    37021 gaaatggagg aaaaataaaa aagacgaacc aatcagcctt tagaaaactg attagttcga
    37081 ggagtaaaaa tgaaaaaagg ttatttggga tgaaactaga ataatgcgta taacttaaat
    37141 aaaacttaaa gcaataaaat ggcaagaggg tgaggcaaat ggaagcagga aaagtattac
    37201 aggaagtaaa agcactatgt gcagataaaa aatatgaaga agcgaaaagc ctgatcgaaa
    37261 agaacaaaga aaatttggaa gaaaagtacc cgatcgcaca agaatttata gaattaaaac
    37321 aaggaacgat ccttcaccga ttcaaacatt tttttggtat aacagaatag aaaaaacgat
    37381 taaagatcgg cagaaatttg ctgcatcttt aatcgttttc tttttaaaca tgcgagggat
    37441 tcaattctga ttcttatata aaaaatagat ccaaataata agaaaaatta ctgaaagcgt
    37501 caattcgatg atgtgtgtta ttaatacaga cgttgtttcc caatgcctaa aagaaaaaat
    37561 ttgataagta aaatatacag caggtaaaga ggccagtaaa actgaaaact tcttttttgt
    37621 ccattttatg taatagaaaa gaatgcttat cataataaaa aaaggaatat aaaaaacaaa
    37681 ttgaccgatc gcgatcatac tattgcctcc gataaataaa gtccatgata caaaataaca
    37741 ctttgtgcga aatggcgcaa gcagttttgt ggatttatcc ggtaaaatct gtatcgattt
    37801 tgaaattaga ggagaaactt gctataatca aagtgaaaga aggagtggga ttttcatgga
    37861 tcatgtagtg ttagaaatcg atttgcattt gatcaataat attcgggtga tttactatgt
    37921 tgtaagtaac agtgtcgaac aacgtgtact gacgaataaa ataaatggta ttttagcaaa
    37981 gaaagacgtt caccgattca ataatggtga aggaagttat tatagtatcc cagtagaaaa
    38041 aattatttat acgacagtca aagtcagaga agatctagat accaaaaaag catatgaacc
    38101 tatttttaca acttattgat tgacagcaga tggaggaaaa aaatgaatcc atatgaacaa
    38161 cttattcaag atttgatcga tggaaaaatc gaaaagattg atgtaaaaag agaagaatta
    38221 atggcttttc gagaagcgtg gctgaagttg gagaatcgta aatatgtggt gggaaaagcc
    38281 tcactgaacg gcaatgtgac ttatcattac gatccgacac gtttatttta gtatgaagta
    38341 ttcagaaaag aaccaaagga ggacgatgca gcagcattgt cctcctttga ttttacaaaa
    38401 gtgaaagttt tacttatttg aatagatttt taaatttgtt tttgacactg tcgatattta
    38461 catcttttaa aagattttgg gctttctctt tataaggtcc taattgttct ttatgttctt
    38521 cgataaatcg ttttgcgcca tcgaaattgc cgttcttaat catttccttg actttttgaa
    38581 ataattcttc ttgattcatt ttgaacacat cctttgctat caacatgatt ttttacatgg
    38641 acaaaacgag aaaaactgat ttatccacac gtcaataata tcaaataacg atcattttta
    38701 ctaaaaaaac actcgattct cgcttctaaa gtaggagaaa aatctccaac ttgggacgga
    38761 tggaatttct ttaactgacc catatactga atatataaca aggaggtaga aagatgaaag
    38821 tgattaaata tttgattgga ctggcggcga ttagtggtat tttgctattt ggactgaaat
    38881 attatactga aggaagttat ggagaaatcc ctggtatact tgatcagcta aatccattag
    38941 ttgaaaaagg agaggtctat gtaaagacac aaaaaccaga ggaagtaaat gaatatggta
    39001 cagcaaggta tgtgcagaaa gctgctgatg ccaatgggaa agaacgaacg gttgaattta
    39061 atggtctttc tgtgttaaaa gaaaatcatt atttgaaaat tagcaataaa ggagcacaca
    39121 tagaaacgta tgaggaagtt cctagagaaa aagttccgga gaaagcatta gcaattattg
    39181 ggtaaatcag tttttgtatt aagaatacgc ccgattttat tattcataat ttaattatgt
    39241 aaactaaata caagtagtaa aaataataca ataatccccc tgcaaaaaga ttgttctttt
    39301 acagggggtt cattttttca gcaagcacat caaattggga tggcagggtt cgaacctaca
    39361 attgcttgat tcagagtcaa gtgccttacc agtttggcta catcccattt attcaacaaa
    39421 ataatcatag cactttccta aaaaaacgca agttattttt cttgatcgga tagttgttgc
    39481 tttactcgta aaaggcggcg tactagtttc ttttctaatc cagcaggaag ggcaatcagt
    39541 tctgctactg acaagacggt gtccaataac tgatattctt tgacaggttc attcataata
    39601 tgatagatgt ttgctaaacg ttcaatatat tcaaagcgag tatactctgt tcgagcaatt
    39661 agaaatttca ataaatcggt ggctttctcg tactcttgat ctgcgtaata aacaagagat
    39721 tgacgataca atttgatttc cagttcattg agggtagcag gtctgcgacg ctcgttccac
    39781 tcattaatag agactttacc ttgaacattt cccttgcagt cacggacaaa aataatcgga
    39841 atttcatttg gatccatttt tttatgtgtg aatttttcca ttatctacgc tcccttcaaa
    39901 ggtttggtat cttgagtata agggattttt gtttgaatta ctagcttttc tccaaagtta
    39961 gcatctgtcc acccattgca gtgaaaatcg ccgaaaaatc gttgatcggt actgctcgat
    40021 tttttacacc atagggatcg ttgacataaa tattttcttt gtcatatcca gtgactacac
    40081 aagcatgaac aagaggagtg actcttactt ctccattatt tgtggtccaa gtcataaaat
    40141 cagtttcatc tgggacttca aaattgatag tcacagaagt ccagacagga tttcctttag
    40201 atagtgcttt tttgatatca tcgagttgag catcggtttt tggaaaagtg tgataatccg
    40261 tttggacgat ttgctgagca agggaggcaa ctgggggaac atagacgacc atagcccagt
    40321 ctccgccact gatatcacca acaaatcctt cattaggatc gccatgagtc ccatctgcat
    40381 tgaaaacagg gacgtaatcc aattgctcag ctaactggtt tttagttgtt tcataaccat
    40441 aaaaagataa aagcatagaa agcgaagtga cttcacagcc attttctaaa gaaggagaat
    40501 caaactggct ctctaatgga acatctagta aaacttctgc tttctctttt gtctttttcg
    40561 ttttttgtgt tgaacttttt tgtgcagtgt tcattgtatg ttctggagaa tggatatgta
    40621 ggtaataact tgtaccgctt aataaaagag cagctgtgcc ccctagaaac aatagttttt
    40681 tgaacgtttt tttcttcatt tgaaatctcc cttttcagct gtttatcttt cctattataa
    40741 atgtattttt attggaaatg gaagtattat attttaatat gtaggaaata attagttttt
    40801 aaggaatatg ttttaatttt ttgaaaaaaa ttcttctaaa tacaacagct gttttaattg
    40861 tgaaaacgaa aacagtacgc tataatgcct gtaaggttga tttttaactg taaataaaaa
    40921 tcaatgagtt aaatctccaa agaaatagca gtggtaatgt ggagaaaagt tccagcagtt
    40981 taggaggatt ttgatgacgg aagaaatgat taatttagga gaacaatatg catgcaaacc
    41041 aattggtttt acaaaaacgg tcattggtga agttgtatca aaaatgacaa actgcgccgt
    41101 ggtcaaagtt gcccaatgtg ctgcagagga ccaagaatta ttagatgaaa aagcaagcat
    41161 ggttgttgca aaatatgata catttgaata aagaaaacaa ttggacgtgc ggcgtgctgc
    41221 aagtccaatt tctttgagaa ggtaaatcag tattcttttc ctattttctg gagaagaaaa
    41281 agcgaggcga gttgtcatgg taacattctt tacggttcta ttgtttttat tcgttgttgt
    41341 gatgttttta tttctttggc tggttcgaaa agaaattatt tatcggactg tgcgaaatcg
    41401 atgggtctac ttggtgatac ccttcttagt gtcactagtc atttggtata cactcatcag
    41461 tcatcctaca gcagatgaat tggcaaaagg gatcttgtca gcaatgattt ttattagttt
    41521 tttgttggac agtagaggaa tcacagaaga aggacttgtt ttgaatagct ttgacaaaaa
    41581 aggtgttcct ttttctgaga tcaataaaat tgtattatat cagccaaaag gttctaaaat
    41641 cgtaaaaatg aattttttcc gaaacggatg gcgaggaccg atgctgaaat tttctgcatc
    41701 attagaagaa ttagttccat ttttgtccca acgcttaaac gatgaggcag agattgatat
    41761 tatgattgat ccagaataaa aacagaggtt gcgacaagat gtttgtcaca acctctgttt
    41821 tttatttcaa ataaaattat ccaaatacgc ggaagcgatc ggcatcgttc atgtattctt
    41881 tttcctcagc cggtgtttct ggtgaaccga atactaattg cgaacgtagt ttccagtttg
    41941 aagggatatt ccattctttt gcaactgctt cgtcaataac tgggttgtaa tgttgcaagt
    42001 ttgcgcccaa tcctttatca actaatgcta cccaagtatt agctgttgcg attccgtttg
    42061 attgttctga ccaatctggg aagttgtctg catataattc aaattgttct tgtagacctt
    42121 ttaccacgtc agtatctttg aagaataata ctgtaccata accatttttg aaaccagcta
    42181 atttgttttg tgtatttggg aaagcttctg ctggagtcaa tgggcgaagt gcttcttcag
    42241 tgatttccca aagtttttca tgtgcatctc cgaaaaggat aactgcgcgt gttgattgag
    42301 cattgaaagc agttgggctt tctttaattg cttctttgat caaagttgtt aattcttcat
    42361 tagaaagtga aacattacgt cctagatgat agattgaacg gcggtttttc aaagtgtcag
    42421 taaaagttgt cataataaaa tttctccttt gatttgcgtt ttgcattttt ttaattgatt
    42481 acgaaatgaa gtatagcacc ttacaaaaag taagtaaaca gatatgctca caaaaaataa
    42541 gcgaagttca aaaaacaatt ttaataagaa aaatcgttat gtaaggattt tcttagttat
    42601 cgctttcatt gttgtgtttt aggaattttt gcttatttga aaaaaacagc tgatatataa
    42661 ttattaaatc tctatcttct tttttttatg atattgccat taaaaattac ttgcttttat
    42721 agaaaattag tttatatttc ttaaataagt tttttagtta aatttttcgg taaagatgta
    42781 taatattttc tgaaaattta aagaattggg gtatagatta tggaaaaaca gcaagacaag
    42841 ggcttcttcg ggcagccgaa gggattgtca actttgttct tcactgagat gtgggaacga
    42901 tttagttatt acggtatgcg tgcattactt atctattata tgtacgatac agttgccaac
    42961 gggggacttg gattaccacg agcaacagca ttagctatca tgtcgatcta cggatctttg
    43021 atctacatgt ctagtatcgt cggcggatgg gtatctgacc gagtgttagg agcaaaacgc
    43081 actgtattct tcggtggagt tttcattatg cttggccaca tcgtcttggc tttaccattt
    43141 ggtgtaagta cattgtttgt ttcgatggca ttgattatta tcggaaccgc ctttttgaaa
    43201 ccaaatgttt ctggcatggt cggacatctt tattcaaaga ctgacttgcg aagagatgca
    43261 ggattttcga ttttttacat gggtgtcaac ttaggggcat tattagcacc actaatcgtt
    43321 ggtacaatcg gtcaagaata taactatcat ttagggttct ctttagctgc tattgggatg
    43381 ttctttggat tattacaata catcatccaa ggacgcaaga cattagacgg agtgggtaca
    43441 acaccgccga atcctttgac aactgaagaa cgtaaaggat tcatccgtaa cttgatcatc
    43501 gcacttgttg ttatcgctgc agtattcggc ggggcacaac taactggtca tctgacaatt
    43561 gacttttttg tcaatacaat cagtgtattg gggattatct taccgttgta ttactttatc
    43621 aaaatgctga cttcaaaaga tgtaactgct gaagaaaaac caagagtatg ggcttatatc
    43681 ccattattcc ttgcagcaat cattttctgg tcattacaag aacaaggttc atctatctta
    43741 gctttatttg ctagtgaacg tacacaatct acgctatttg gtgtaacgat ccaagcaagc
    43801 tggtatcaat cattgaatcc aattttcatc tttattctga caccagtatt cgtaacgatg
    43861 tggacgaaac ttggtgaacg tcaaccatct acagtagtga aattctctct tggactttta
    43921 tttgcaggat tatcgtactt gctattgatg ttgccgggta tgctttatgg tacaagtgga
    43981 aaagtaagtc cattgtggtt gttgggaagt ttcttcatcg tggttattgc tgaattgtgt
    44041 ctttcaccag ttggtttgtc tgtgacaaca aaattagcac cacgagcatt tgaatcacaa
    44101 acaatcgcta tttggttctt agctgatgct tcttcacaag caatcaatgc acaaattgca
    44161 cgcttctaca caccgggaac agaatctgct tacttcggta ttatcggtgc agtagcaatc
    44221 gtcggtggta tcttgctatt tactgtgaaa gaaccaatca aaaaactaat gggctctatt
    44281 cattaatcaa tgaacataaa aacaggaatt gcttatttgc agcagttcct gttttt
//
LOCUS       DASHHB010000021        42961 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.021, whole genome shotgun sequence.
ACCESSION   DASHHB010000021 DASHHB010000000
VERSION     DASHHB010000021.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 42961)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 42961)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..42961
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.021"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(151..2304)
                     /locus_tag="U2K71_001218"
     CDS             complement(151..2304)
                     /locus_tag="U2K71_001218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HEM7315314.1"
                     /translation="MMKDFMKKILNTSWLEKIRKGAQEPKNKTFRRSHVPFRLNFLFF
                     IIFTLFVALIARLGYLQIVNGEEMEARVKSTSTITVQESTPRGMIYDSTGKALVTNKA
                     NQAITYTRGTQVTAKDLLEIATKLNSLIDVPVDENLTDRDKKDFWLADPKHLKEATKR
                     LSAKEKQLGNSEQYSAMVNKVTADEINLNEEQLKIATIFKRMNSAYELNTVYIKNSGV
                     TDQELAVVAERASELPGVSTGTDWTREYNAASSLKSILGSVTTEKQGLPADEAEKYLA
                     KGYSRNDRVGQSYLEKQYEDVLQGTKTQYEVSLDNEGNVSNQKEIFSGEKGSNLMLSM
                     NAEFQSKVEEILKRNYQTLINNGKAQYSPGAYAVAMNPQTGEVLAMTGFSHEQGSKEI
                     TENALGTITSAFDPGSVVKAGTLTAGWASNAISGNQVLIDEPIRLQGASEKSSVFNRS
                     GQVALDAVKASELSSNTYMIKVALKMLGLDYTPGMGLPSLDEEAKAYQQLRDSFKEFG
                     LGTTTGIDLPNESPGISRSVDYMKKFNADNGKEWYAPGNFTDLAFGQFDTYTPIQLAQ
                     YASTVANGGKRVQPHLVKAIYGNDENGNLGEVKKEIGTTVENTVNISAENMAILREGF
                     HQVVHGTDPYTTAKPLASAKMDLSAKTGTAETVAEGHPDIATVNSNIVAYGPTDNPEI
                     AISVVLPNLLDEQDHINLTIAKEIMDAYYDMFMAK"
     gene            complement(2598..3446)
                     /locus_tag="U2K71_001219"
     CDS             complement(2598..3446)
                     /locus_tag="U2K71_001219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM7315315.1"
                     /translation="MSVISRIESMMEDYSSAEKKLANYIIGNIEKVPTMTANELAEAS
                     GLSAPTVVRFSKKIGFQSLTDFKITISTELQTGIDEGFSDIEANESFYSIKNKLGNNA
                     QVAIKETVDILEEETIQKVVECLESAETVFLYGVGASSLVVEDILQKWSRVGKPIIFE
                     KDIHVLLPQLVSNEKKKVLWLVSNSGRSADVVALAELAKSMNIEIIALTQFGNNPLSK
                     IADVLVQTSRPKEITNRSAATNSLLAQFATIDIIFYFYMAKNEKLSEKVKKTREAIQE
                     YFMDSK"
     gene            complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="U2K71_001220"
     CDS             complement(3670..5610)
                     /gene="thrS"
                     /locus_tag="U2K71_001220"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HEM7315316.1"
                     /translation="MIKITFPDGAVKEFESGTTTLAIAESISKSLAKKALAGKVNGKL
                     VDLTRPIEEDASIEIITPDHEDALGLVRHSAAHLMAQAMRRLYPNIHFGVGPAIDSGF
                     YYDSDNGQNQVTAEDLPAIEAEMMKIVKENLPIERRVLSKQEALEIFASDPYKVELIS
                     ELPEEEVITAYQQGEFIDLCRGPHVPSTGRIQVFKLLSVAGAYWRGNSNNQMMQRVYG
                     TAFFDKKALKEYIRLREEAKERDHRKLGKELDLFMVSPEVGSGLPFWLPKGATIRRTI
                     ERYIVDKEVSLGYQHVYTPIMGDVELYKTSGHWDHYQEDMFPPMDMGDGEMLVLRPMN
                     CPHHMMVYKNTIHSYRELPIRIAELGMMHRYEKSGALSGLQRVREMTLNDGHTFVRPD
                     QIKDEFKRTLELMVAVYADFNITDYRFRLSYRDPNNTDKYFDDDAMWEKAQAMLKAAM
                     DELELDYFEAEGEAAFYGPKLDVQVKTALGTEETLSTIQLDFLLPERFDLTYVGEDGE
                     NTHRPVVIHRGIVSTMERFVAYLTEVYKGAFPTWLAPIQATIIPVSVDAHGDYAYEIK
                     ERLQMKGLRVEVDDRNEKMGYKIRASQTQKIPYQLVVGDKELEDATVNVRRYGSKETA
                     VEDLNIFIDAMEAEVKNYSREN"
     gene            complement(5947..6126)
                     /locus_tag="U2K71_001221"
     CDS             complement(5947..6126)
                     /locus_tag="U2K71_001221"
                     /EC_number="5.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016252354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase"
                     /protein_id="HEM7315317.1"
                     /translation="MPFVHVELVEGRSPEQLENMMKDITEAVHKNTQAPKEHIHVIIN
                     EMKKGTYGVNGEWKK"
     gene            complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="U2K71_001222"
     CDS             complement(6173..7996)
                     /gene="cadA"
                     /locus_tag="U2K71_001222"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEM7315318.1"
                     /translation="MTHFKKFLLTLLIGGIALLSEFVFHQPALAFAIVAFTGGILAFL
                     MFLEMIKTLRSGRYGVDVLAITAILATLAVKEYWASLMILIMLTGGDSLEDYANKRAG
                     KELQSLLDNTPRIAHRFKEGKLQDIVIDEINIDDQLLVKPGELVPADGRVTKGESSVD
                     ESSLTGESRPETKKPGDQIMSGSLNGDGSIQMIAEKRAADSQYQMIIKLVEESKEKPA
                     RFVRLADRYAVPFTLIAYLIGGISWWFSKDPLRFAEVLVVASPCPLILAAPIALVAGM
                     SRSSKNGIVVKTGTTIEKLARTKTIAFDKTGTLTKGILEVEAIQPVNGIKKEELLLYA
                     ASAEQESSHILARSLVNNVKNSQLYPVSQLKEVTGQGIQAVVNNHEVKIGRASFVSAE
                     EIETSNTAIYVTIDRSFAGTILFSDQIRPEAAKTIKELRKLDVKNLMMITGDGSAIAQ
                     TIAEKVQLDSVHARSLPQDKLTILESIPKDQRPVAMVGDGVNDAPALAAADIGIAMGA
                     HGSTAASESADAVILKDDLLRVAKAVVISRDTMRIAKQSVLIGIFICIFLMLIASTGL
                     IPALFGAVLQEVVDTVSILSALRAKKDPAIFPDSVPKTNIV"
     gene            8274..10553
                     /locus_tag="U2K71_001223"
     CDS             8274..10553
                     /locus_tag="U2K71_001223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM7315319.1"
                     /translation="MNEKQLEQTHVDETIELIQIEQNMLDTKQQELTQEMNHSTKDTA
                     NHKIRAGSNEAFYESAVEYRQHEQELLLKYHTLEAQQKRLQTLSVMKDSPYFARIDFK
                     EDRETETLYLGIASLRDLAEETIVIDWRAPIANLYYEGEIGPAFYETETDRIDVDLQL
                     KRQFKIQEGRILSMVDTSEVINDDFLLEILDESSSNHMKNIVSTIQKAQNAIIRDTTS
                     KVMLIEGIAGSGKTSALLQRIAFLLYHNRKWLEAENVLLFSPNHLFSDYISTVLPSLG
                     ESGVPTQTFKNYLVQLLPEFSLLDEQQQESGFLLGEKDPIQVMKSGLTLVDQIDRYIQ
                     GITNYGPLFRDMKINGRTILSKESIRQWYKETNEQLPLHHRLSLLQTKLLKKLGGLQK
                     DETRQQWVKDLAEEQLQELYATDPNLEYTEQKERSLRKKLANQIVRKRFRRIHRGIMN
                     YQFVNITKQYLHFLQTVPLSLLTRHKISETRWQEHLTTVRTHLRQRELRQEDAVLFFL
                     LMRRTYPVTVTHKARYIFIDEMQDFAPSQVALLQALFPKANLTFCGDLNQNVFGNETI
                     VGSLEDLFVDREVTRFQLTTSYRSTKEITDFANHFIVKEHQVETTARKGDLPLLIQGT
                     TDDIKLNWLADTIRTTQGKARYWRTAIIAKTQEECEALYAQLPDELKQQVQLILNESD
                     FMKRSIILLPAFLAKGLEFDRVFLWDVDAEHFSSQQDKLILYTMCTRAMHELTLFTSN
                     NESPLLNNMDISSYEKKVL"
     gene            complement(10646..11293)
                     /locus_tag="U2K71_001224"
     CDS             complement(10646..11293)
                     /locus_tag="U2K71_001224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315320.1"
                     /translation="MEAKITLEPFERILSGYRKVEELAVNVTDCSKLAQKYARFGVEG
                     YRLGNYVGTGYLNRYLECMVDRAPMLIYRQNYLIPLLFRRSDSAFRLFEEEYRMEAFF
                     LLLEWSLKHRPEKILIERNEKIDTKKNKVIDSAYLAFRVSEILDCGGYPISNFQSIDQ
                     FIEWNRIYRLIDNGGIGRHSKVFDPEYPENMEELKMIISLVKLKYPETDLDLYIE"
     gene            complement(11472..12191)
                     /locus_tag="U2K71_001225"
     CDS             complement(11472..12191)
                     /locus_tag="U2K71_001225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7315321.1"
                     /translation="MGRKWANIVAKKTAKDANNSRIYAKFGIEIYAAAKSGDPDPHAN
                     QKLRFVIDRAKTYNVPKHIIDRAIEKAKGAGDETYSELRYEGFGPSGSMVIVDTLTNN
                     VNRTAADVRAAFGKNGGNMGVSGAVSYMFDNTAIFAFAGEDADEILEYLMEKDIDVRD
                     VMEEEGQIIVYGEVADFHSIQEALKEKGITDFSIAEIQMIPQNEVTLSPEDQETFEKM
                     IDALEDLEDVQHVFHNVALED"
     gene            complement(12322..13473)
                     /locus_tag="U2K71_001226"
     CDS             complement(12322..13473)
                     /locus_tag="U2K71_001226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase"
                     /protein_id="HEM7315322.1"
                     /translation="MKSCGIIVEYNPFHNGHRYHVEMARKITGAEVVIAVMSGNFLQR
                     GEPAIIDKWHRANEALQNGVDLVVELPVEWAVQSADYFAKGAVKILQTLDCDSLCFGT
                     EEETSFDYSSFGRFVQENQDRIEEAFQELSDQTYSYPQKMTEVFRSLYPDITLDFSSP
                     NHILGLSYAKENAKYVHPMELHPIRRKSAGYHEKIITDQRIASATAIRHALEEGKRIA
                     GLVPPQTEEDLSTCSLQTWESYWPYLRYRLLSSTLLELTDIYQMTEGIESRMKTAAAT
                     ADSFEDFVSKVKTKRYTWTRIQRLSCYVLLNIKKQEIPVQQNKNYLRLLGFTDAGRRF
                     LKEKKEISIYSRIGKKEAQEAELLVRSDQIYQLGGNIPEQNFGQIPIRV"
     gene            complement(13690..14430)
                     /locus_tag="U2K71_001227"
     CDS             complement(13690..14430)
                     /locus_tag="U2K71_001227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM7315323.1"
                     /translation="MAYETFAFIYDEVMDETLYQQWLAFSKRHLQEDTKNILELACGT
                     GALAVAFAKDGYKVTGLDLSEEMLMIASQRAYEEDASIQFVEGNMLDLSEIGQYQAIT
                     CFSDSLCYMKNRQEVQQVFDEVYQALEENGRFLFDVHSTYQVDEVFPEYSYHYQGDNF
                     AFLWDSYPGSEKHSIEHFLTFFVEEQEGSGRFIREDELHQERTYPLESYLRMLENSGF
                     SKVEAFSDFTDEAASEKSKRWFFVAYKD"
     gene            complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="U2K71_001228"
     CDS             complement(14434..14757)
                     /gene="rsfS"
                     /locus_tag="U2K71_001228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HEM7315324.1"
                     /translation="MEAADSKRAEDIIALDVRNVSLLADYFMICSANSERQINAITEE
                     IIDKEEENKYEVKRIEGKEGGKWVLIDLGDVIVHVFHAPERNFYNLEKLWSDAPLVDL
                     SEWLD"
     gene            complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="U2K71_001229"
     CDS             complement(14814..15407)
                     /gene="yqeK"
                     /locus_tag="U2K71_001229"
                     /EC_number="3.6.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bis(5'-nucleosyl)-tetraphosphatase (symmetrical)
                     YqeK"
                     /protein_id="HEM7315325.1"
                     /translation="MNMDYSNEYTAYSRENLMQKIQMRMSEQRFKHVLGVEETAVALA
                     KKYGASPEKASIAALTHDYAKERPDEEFKMVIVRDGFDPELLNYNNAIWHGLVGASFV
                     ERELGITDAEILHAIRVHTTGAAKMSLLDKIIYVADYIEPGRDFPGVQDARAIAWADL
                     DEAVAFETKHTLAHLLAQEQQIYPKTIETYNYWVAKK"
     gene            complement(15391..16041)
                     /locus_tag="U2K71_001230"
     CDS             complement(15391..16041)
                     /locus_tag="U2K71_001230"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HEM7315326.1"
                     /translation="MMNTQAKTFVRSQLFPEEMPQFLEKKKQVGILGGTFNPVHLAHL
                     VMAEQAGRNLGLDRVFLMPSYQPPHVDEKQTIDAKHRLNMLELAVEDNPFLQIETIEL
                     ARGGKSYTYDTMKELTQNNPDTDYYFIIGGDMVEYLPKWYKIDELTSMVNFVGIRRPG
                     YTTDTPYPVIWVDVPEIDISSTKIRQKIKEGCSIRYLVPDKVIDYIQNEGLYEYGL"
     gene            complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="U2K71_001231"
     CDS             complement(16051..16362)
                     /gene="yhbY"
                     /locus_tag="U2K71_001231"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome assembly RNA-binding protein YhbY"
                     /protein_id="HEM7315327.1"
                     /translation="MSLRGKQKRFLRSKAHHLQPIFQIGKGGINSAMIVQIEEALEKR
                     ELIKVSLLQNTDEIAEEAAQVLEKEIDCEIVQIIGRVIVLYKPSTKEKYQRLSKEVKA
                     I"
     gene            complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="U2K71_001232"
     CDS             complement(16378..17487)
                     /gene="yqeH"
                     /locus_tag="U2K71_001232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase YqeH"
                     /protein_id="HEM7315328.1"
                     /translation="MTEELQCIGCGAVIQTNKPGELGYTPQTALEKGLASGEVYCQRC
                     FRLRHYNETQDVSLTDDDFLRLLNELGQKDALIVNVVDIFDFNGSLIPGLHRFVGDNP
                     VLLVGNKVDILPKSLKKTKLTQWMKERAYEAGIRPVDVLLTSAKKSHEMTELLEKIEE
                     YRDGRDVYIVGVTNVGKSTLINQIIKNTAGVQDVITTSQFPGTTLDKIEIPLDDGHFL
                     IDTPGIIHRHQMAHYLGKKDLRIIAPLKEIKPKVYQLNEGQTLFLGGLARFDFISGNR
                     ASFIAYVSNDVNIHRTKLEKADEFYQKHVGGLLQPPRPDEVEAFPELVRFEFSVKEKT
                     DIVFAGLGWITVTAPVVIAGWAPKGVDVLKRKALI"
     gene            complement(17484..18011)
                     /locus_tag="U2K71_001233"
     CDS             complement(17484..18011)
                     /locus_tag="U2K71_001233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqeG family HAD IIIA-type phosphatase"
                     /protein_id="HEM7315329.1"
                     /translation="MFSNYKPTWMVEAIYQITPAQLKNLGIKAVLTDLDNTLIAWNNP
                     DGTEELLTWILEMKNAGIPVVVVSNNNSKRVARAIEKFDLLYVARAMKPLARGINIAK
                     KQLDLADDEIVMVGDQIMTDIRGANRAGIRSILVKPIIATDSWKTQFNRFWEKRIMAY
                     LLAKHPDMGWRGGIE"
     gene            complement(18191..18976)
                     /locus_tag="U2K71_001234"
     CDS             complement(18191..18976)
                     /locus_tag="U2K71_001234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyl transferase
                     subunit alpha"
                     /protein_id="HEM7315330.1"
                     /translation="MDKTAHDVVQLARAKERWTSLDFFEEIFDSFEECHGDRLFADDE
                     AIVGGIARLDGKPVTVIGIQKGRDLQENIRRNFGSPHPEGYRKALRLMKQAEKFNRPV
                     ITLINTAGAYCGVGAEERGEGEAIARNLLEMSDLKVPIIAIIIGEGGSGGALALGLAD
                     EVWMLEETIYAVLSPEGFASILWKDGSRAAEAAELMKITAPELRELGIVDRVIPETFN
                     GEQLPRYKVAHIMKKALIERLTELVRLDQHKLLEKRYKRFRQY"
     gene            complement(18969..19838)
                     /locus_tag="U2K71_001235"
     CDS             complement(18969..19838)
                     /locus_tag="U2K71_001235"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase carboxyltransferase
                     subunit beta"
                     /protein_id="HEM7315331.1"
                     /translation="MALFKKKKYIRINPNRESKPANSPSVPDNMWAKCPNCKHILYTK
                     DIGEEKVCPHCGYAFRIGAWQRLALIIDEKSFEEWDTDLVTKDPLAFPEYTEKIKKMQ
                     DKTGLHEAVLTGKATIQGIPFIIGVMDPNFIMGSMGTIVGEKITRLFERATKESLPVV
                     LFTASGGARMQEGIFSLMQMAKISAAVKRHSNEGLFYLTVLTDPTTGGVTASFAMEGD
                     IILAEPQSLIGFAGRRVIEQTIKQELPEDFQKAEFLLSHGFVDQIVPRMELKQKIHTL
                     LELHTQKGWIDRG"
     gene            complement(19839..21218)
                     /locus_tag="U2K71_001236"
     CDS             complement(19839..21218)
                     /locus_tag="U2K71_001236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HEM7315332.1"
                     /translation="MFSKVLIANRGEIAVRIIRACRELGIQTVAIYSEADAEALHTQL
                     ADEAICIGPAKAVDSYLNVQEVLSAAIVTKAEAIHPGFGFLSENSRFASMCEECNITF
                     IGPKSATIDAMGNKINARQLMQEANVPVIPGSTGVLSTVEEALEIADRIGYPVMLKAA
                     AGGGGKGIRKVQSKEELPQHFSSAQQEAAAAFGNDDMYLEKIIYPARHIEVQILGDRF
                     GHVIHLGERDCSLQRNNQKVLEESPSVVISQTKREALGDAAVRAAKAVNYENAGTIEF
                     LMDEEGDFYFMEMNTRIQVEHPVTEMVTGIDLVKKQVEIAAGEPLNVCQEEVVFQGHA
                     IECRINAENPAFHFAPSPGKIQNLLLPAGGMGLRVDSAVYPGYTIPPYYDSMIAKIIV
                     HGNTRFEALMKMQRALSEFITEGIITNAEFQMDLISHPAVIAGDYSTAFLQEEFLPNW
                     TPETEIGGA"
     gene            complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="U2K71_001237"
     CDS             complement(21226..21645)
                     /gene="fabZ"
                     /locus_tag="U2K71_001237"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEM7315333.1"
                     /translation="MNIQEIKEIIPHRYPMLLIDRVEEMVEGERIVAKKNVTINEPFF
                     QGHFPEEPVMPGVLIVEAMAQAGAVALLSLEQFKGKTAYFGGLDKAKFRKKVTPGDTL
                     YLEVEILKVKASAGIGKGIAKVDGKKVAEAELTFMIG"
     gene            complement(21670..22146)
                     /gene="accB"
                     /locus_tag="U2K71_001238"
     CDS             complement(21670..22146)
                     /gene="accB"
                     /locus_tag="U2K71_001238"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HEM7315334.1"
                     /translation="MDINEIRELVSQFDQSSLTEFDLREGQFELYMNKNKTSRGMNMV
                     QTNETLPVSEPVNVIPEQATTAPEIMKEKESAVFDGIEVVSPIVGIVYLQPAPDKPAF
                     KKVGDQVAKGEVICIIEAMKLMNEITSEADGVITEILVENESVVEYGQPLFRIQQK"
     gene            complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="U2K71_001239"
     CDS             complement(22149..23384)
                     /gene="fabF"
                     /locus_tag="U2K71_001239"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HEM7315335.1"
                     /translation="MNRVVITGYGVTSPIGNDADSFLNSLKEGTNGIAPITKFDASET
                     GITLAGEVKDFPYDKYFIKKDSKRMDMFSIYGIYAALEAMEMSGLDKEKMNQDRFGVI
                     IGSGIGGLPTIENQVIRLHEKGAKRVSPMFVPMAIANMAAGNIALRVGAKGICTAIVT
                     ACASGTNSIGEAFRNIKHGYSDVILAGGTEATICEMGIAGFAALTALSESTDPNRGSI
                     PFDENRNGFVMGEGAGVLVLESLEHAQARGAKIYGEIVGYGANCDAYHMTAPTPDGSG
                     ASKAMKLAMEEAGVQPEQVGYINAHGTSTPANDQAEAKAIQIALGENFRDTYVSSTKS
                     MTGHLLGAAGGIEALATLLALEHQFIPPTINVEKQDPEIDLTVVKNESKPASFTYAMS
                     NSLGFGGHNAVLCMKRWEE"
     gene            complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="U2K71_001240"
     CDS             complement(23403..24140)
                     /gene="fabG"
                     /locus_tag="U2K71_001240"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HEM7315336.1"
                     /translation="MDVKGKNVFITGSTRGIGKAMALAFAKAGANIILNGRGEIPKEK
                     IEEIEAFGVKCVGVSGDISDYEKAGQMIKEAEEKLGSIHVLVNNAGITNDKLVMRMDA
                     EDFKKCLDINLIGTFNMTQHVLKKMMKQREGAIINLSSVSGLIGNIGQANYAASKAGV
                     VGLTKSVAREAATRGITCNAIAPGFITTDMTEVLADKVKEQAEKQIPMQRFGQVEDIA
                     QTAVFLAQNPYITGQVINVDGGLVMHG"
     gene            complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="U2K71_001241"
     CDS             complement(24152..25069)
                     /gene="fabD"
                     /locus_tag="U2K71_001241"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HEM7315337.1"
                     /translation="MKTAFLFSGQGAQYQGMGKDLYEEAVVKQTFDEASEILGYDMAE
                     LCFTENERLDQTQYTQPAILTVSIAYYRLLKEHGIIPDAALGLSLGEYSALVASNALS
                     FTEAVALVAKRGAYMTEAAPAGSGKMVAVMNAPIETIEESCHEASKYGIVSPANYNTP
                     QQIVIGGEEKAVDEAVLLLKEKGFKRMIPLNVSGPFHTAILEPAAKKLAKDLSQIQFS
                     EPSFPIISNTTTEIMKKETIAGLLEQQVMQPVRFYESIHKLKTIGIEQVIEVGPGKVL
                     SGFMKKIDKTIPVLRVENKQTFDETIAIL"
     gene            complement(25176..25400)
                     /locus_tag="U2K71_001242"
     CDS             complement(25176..25400)
                     /locus_tag="U2K71_001242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEM7315338.1"
                     /translation="MVFEKIQEIIAEELGKETEEIKLTTDIQEDLEADSLDLFQIINE
                     IEDEFDVKIETEDGIKTVQDLVTYVEKQTA"
     gene            complement(25453..26415)
                     /locus_tag="U2K71_001243"
     CDS             complement(25453..26415)
                     /locus_tag="U2K71_001243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketoacyl-ACP synthase III"
                     /protein_id="HEM7315339.1"
                     /translation="MEQFGMITATASVLPEKIITNNDLSKMMDTSDEWISSRTGIRER
                     RCVTNQETSDLCILVAEKLIKQKNLNPKDLDFILVATMSPDYTTPSVAAQVQGKLGAT
                     HAIAFDISAACSGFVYALSMAEKMIRCGSTKGLVIGGETLSKLIDWSDRTTAVLFGDG
                     AGGILLEANPQKKLLKEKLAADGTRSSSLTAGYQQNKNPFYSEDSEGSYYLQMTGRDI
                     FDFAVRDVANNIQEVMKDKPVDYLLLHQANLRIIEKIARKVKMPQEKFLTNMDKYGNT
                     SAASIPILLDEAVSSGKITLGKQQKVIFTGYGGGLTWGSILMEL"
     gene            complement(26425..26865)
                     /locus_tag="U2K71_001244"
     CDS             complement(26425..26865)
                     /locus_tag="U2K71_001244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005471259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM7315340.1"
                     /translation="MEPDLKTVNDYLVSVFNDILTIEESELKKSQFKDLSITEMHTIE
                     AIGMYKKKTTSEVAKELSITVGTLTTAINKLVKKGYVERIRSEDDRRVVKLGLTKKGK
                     LLFRVHQHFHREMVKNILDGMATEEQHALLDALKNLHDFLQDYK"
     gene            27171..28136
                     /locus_tag="U2K71_001245"
     CDS             27171..28136
                     /locus_tag="U2K71_001245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GIY-YIG nuclease family protein"
                     /protein_id="HEM7315341.1"
                     /translation="MLKEKMKTLPMLPGVYLMKDRDGKVIYVGKAKRLKNRVSSYFHQ
                     NKQHSKKVLRMIHHITDFDFVVVDTELDALLLECQLIQHYRPFYNRQMNYFSNYNYVH
                     ITNKGFVLTDTPTARTYGPFRLYKKMPSILRIMEETYQMPWLSEISLLALRVQLPDLQ
                     EMTFEQKKKELQGLFQGRNKKLLTYLKKRQQHFIYQLNFEKAGMLQKDIELVTYFIRR
                     IQEQKQFLRTPSLTFSMPLAADESQKKHYLVCYGQLAETMITSGDVSPDFYYEKKEAH
                     LSLKRQLSKEEIDPVQILISYRKKLEKEQFEIELLNKKEAEKQLN"
     gene            complement(28193..28423)
                     /locus_tag="U2K71_001246"
     CDS             complement(28193..28423)
                     /locus_tag="U2K71_001246"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YkuJ family protein"
                     /protein_id="HEM7315342.1"
                     /translation="MTHSQLVAIIKRLEAMVESADNELQVRRFEKEGVEKCTVTYDKA
                     TDTFELTETDTHQQYEFDNIDIVAMEIYDLIQ"
     gene            complement(28509..29395)
                     /locus_tag="U2K71_001247"
                     /pseudo
     CDS             complement(28509..29395)
                     /locus_tag="U2K71_001247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301386.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
     gene            complement(29484..30707)
                     /locus_tag="U2K71_001248"
     CDS             complement(29484..30707)
                     /locus_tag="U2K71_001248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010706866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM7315343.1"
                     /translation="MKIGFFTDTYFPQVSGVATSIKTLKQELERHGHEVYIFTTTDPN
                     ADKLEKDVIRMPSVPFISFKDRRVVVRGMWYAYLVAKELELDLIHTHTEFGAGILGKM
                     VAKKLKIPLIHTYHTMYEDYLHYIGKGKVIRQSHVKYLSRLFANHTTGVVCPSERVID
                     TLRSYGVIAPLRVIPTGIDIEKFKRSDITQQDISKLRESLGLQENHLMILSLSRISYE
                     KNIQALINGFPQVISAYPNARLVIVGKGPYVEELEELISELQLEEFVQFTGEVDNNDV
                     ALYYKAADYFVSASTSETQGLTYTEAMAAGTPCVVEGNAYLNRLFDHPSLGKTFETDE
                     DFAPALIDYISSEVPKDPKVLEAKLYDISSTHFGEAMIEFYQDMVVYHEEEMRRKEEE
                     DSIERIKIKLNSFKR"
     gene            complement(30724..31770)
                     /locus_tag="U2K71_001249"
     CDS             complement(30724..31770)
                     /locus_tag="U2K71_001249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM7315344.1"
                     /translation="MKVLLYFESEKMLAKSGIGRALDHQKRALTEVGISYTLDEKEDY
                     DILHINTYGINSHNMVNKARREGKKVVYHAHSTEEDFRNSFIRSNQLSPIVKKYLVGL
                     YQKADYLITPTPYSKQLLESYGIRVPIQAISNGIDLEKYHPDPMKEQKFREYFKLSPD
                     QKVIICVGLFFERKGIIDFVEIAKKMPEYTFIWFGHVPMYSIPRNIRKIVKEDHPENV
                     LFPGYIRGEIIEGAYSGADLFFFPSYEETEGIVVLEALASKQNVLVRDIPVYNGWLED
                     SKNCYMGKTNEEFIRLIQQITNQELPSTTQAGYQTAKERSINKIGEELKNVYESVLNE
                     KVSSKLKKVNQVKD"
     gene            complement(31934..33661)
                     /gene="ptsP"
                     /locus_tag="U2K71_001250"
     CDS             complement(31934..33661)
                     /gene="ptsP"
                     /locus_tag="U2K71_001250"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HEM7315345.1"
                     /translation="MVEMLKGIAASDGIAVAKAYLLVQPDLTFSKATVEDTAAEEARL
                     DAALAKSTEELQQIREKAAQSLGEAEAQVFDAHLMVLSDPEMVGQIKQNIKDNSVNAE
                     SALKEVTDMYIGMFEAMEDNAYMQERAADIRDVAKRILAHLLGVTLPNPSMINEEVVV
                     VAHDLTPSDTAQLDRNFVKAFVTDIGGRTSHSAIMARSLEIPAIVGTKEITAKVKEGD
                     ILAVNGIEGDVIIDPTDEQKAEFEKAGADYAAQKAEWEKLKNAETVTADGKHFELAAN
                     IGTPKDLVGVHNNGGEAVGLYRTEFLYMDSPDFPTEDDQYEAYKAVLEGMEGKPVVVR
                     TMDIGGDKELPYLQLPHEMNPFLGYRALRISLSEQGDEMFRTQMRALLRASVHGNLRI
                     MFPMVATLKEFRAAKAIFEEEKQKLISEGKEVSDTIQVGIMIEIPAAAVLADKFAKEV
                     DFFSVGTNDLIQYTMAADRMNERVSYLYQPYNPSILRLIKNVIDAAHAEGKWAGMCGE
                     MAGDQTAVPLLVGMGLDEFSMSATSILKTRSLMKRLDTAKMAELADRALKECDMMEEV
                     VELVHEYVK"
     gene            complement(33661..33927)
                     /locus_tag="U2K71_001251"
     CDS             complement(33661..33927)
                     /locus_tag="U2K71_001251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010773230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr"
                     /protein_id="HEM7315346.1"
                     /translation="MEKKEFHVVAETGIHARPATLLVQTASKFNSDVNLEYKGKSVNL
                     KSIMGVMSLGVGQGSDVTITAEGADEADAMAAIVETMKKEGLSE"
     gene            34406..36640
                     /locus_tag="U2K71_001252"
     CDS             34406..36640
                     /locus_tag="U2K71_001252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM7315347.1"
                     /translation="MLCQNCGKNEATIHLYATVNGQRTQLDYCQSCYQKIKNQQNGGL
                     TNMAQNDPFGFGSLDDLFRSMSRQMQQQGNYEQTPPTQFGGNNNFNGGQPPQGNTEGL
                     LGEYGINITEQARQGDIDPVIGRDDEIKRVIEILNRRTKNNPVLIGEPGVGKTAVVEG
                     LAQKIVDGDVPQKLMDKEVIRLDVVSLVQGTGIRGQFEERMQKLIEEIRQAENVILFI
                     DEVHEIVGAGSAGDGNMDAGNILKPALARGELQMVGATTLNEYRIIEKDAALERRMQP
                     VRVDEPTVEETISILKGLQKRYEDYHHVKYTDEAIEAAATLSNRYVQDRFLPDKAIDL
                     LDETGSKKNLTIQIVDPKTIEKKLQEAEEQKVLASREEDFEKAAYYRDQINKLQKMKE
                     RQLTEEETPVITEKDMEKIVEQRTGIPVGELKEKEQTQLKNLADDLKAHVIGQDNAVD
                     RVAKAIRRNRVGLNKQNRPIGSFLFVGPTGVGKTELAKQLAYELFGSQDSMIRFDMSE
                     YMEKHSVSKLIGSPPGYVGYEEAGQLTEKVRRNPYSLVLLDEVEKAHPDVLHMFLQIL
                     DDGRLTDAQGRTVSFKDTIIIMTSNAGTGKVEANVGFGAAREGVTRSVLNQLNNYFTP
                     EFLNRFDGIIEFSALSKENLMTIVTLMLDDVNQMLAAQQLHIEVPTNVKEKLVDLGYD
                     PSMGARPLRRTIQEQIEDGIAEFYLDHPSIHELKAKLDKDGKIIVTSKPERLAKESAE
                     ETAE"
     gene            36788..37108
                     /locus_tag="U2K71_001253"
     CDS             36788..37108
                     /locus_tag="U2K71_001253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEM7315348.1"
                     /translation="MKLVNVTNSHSRLVLNQLENTDAHLVKVYTAGNTTIVYTEAPEH
                     NEILLINDKRKIQPKEIEDAKNYFLDKIKDAVYHEDEIKVIELAGLVEISIPKHELGA
                     TLAV"
     gene            complement(37313..37537)
                     /locus_tag="U2K71_001254"
     CDS             complement(37313..37537)
                     /locus_tag="U2K71_001254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein"
                     /protein_id="HEM7315349.1"
                     /translation="METEWQRRERKKQKKEEMLRLCRLLDVKDWDKNEQIAQKVRSII
                     HKEEKVKNKDTRRTENEKRKVQKKREKSKS"
     gene            complement(37735..39315)
                     /locus_tag="U2K71_001255"
     CDS             complement(37735..39315)
                     /locus_tag="U2K71_001255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 3"
                     /protein_id="HEM7315350.1"
                     /translation="MNNPKLKQQVDNRRTFAIISHPDAGKTTITEQLLLFGGAIRQAG
                     TVKGKKTGNFAKSDWMEIEKQRGISVTSSVMQFDYQGKRVNILDTPGHEDFSEDTYRT
                     LMAVDSAVMVIDSAKGIEAQTKKLFQVVKKRGIPIFTFINKLDRDGREPLELLEELEE
                     LLDIESYPMNWPIGMGKGLEGLYDIYHNRVEFYRPENYQDERLVELDEDGLLPENHPL
                     TENSLYEQVLEEVELIKEAGDAFNQEKIARGDQTPVFFGSALTNFGVQTFLETFVELA
                     PAPYGHKTQDDQMVSPYEEEFSGFVFKIQANMNPAHRDRIAFVRICSGTFERGMDVWL
                     ERTNKKLKLSNVTQFMADSRENVEKAVAGDIIGVYDTGNYQIGDTLFEGKLKVAYEEL
                     PSFTPELFMKVTAKNVMKQKSFHKGIYQLVQEGAIQLYKTYLTEEYIIGAVGQLQFEV
                     FQYRMLNEYNAEVIMSPMGHKIARWIDPEDLDEKMSSSRNILARDRFDQPLFLFENQF
                     AERWFADKYPNVKLKSLM"
     gene            complement(39716..41092)
                     /locus_tag="U2K71_001256"
     CDS             complement(39716..41092)
                     /locus_tag="U2K71_001256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEM7315351.1"
                     /translation="MNADPESQSLLAQILLLIVLTLINAFLAASEIAVVSINKNRIEQ
                     KADEGDVKSQKLLKILQNPNNFLSTIQVGITLVNILSGASLANTLSERLAPVLGGGTA
                     AKNIANIIVLAILTYVSIVFGELYPKRIALNKSEEVAKFTSGLIRLIGVVAKPFVWLL
                     SASTSLLARITPMTFDDEDSKMTRDEMRYMLENEGVLNNEELEMLQGVFSLDTKVARE
                     VMVPRTDAFMIDINDTVEENVNEVLSENYSRIPVYNEDKDKVVGILHTKNLLKAAHKF
                     GFDNLDIKKIMQEPLFVPETVFIDDLLYEMKKTQNQMAILLDEYGGVVGLATLEDLLE
                     EIVGEIDDESDEVENLYEKIDEHEYIIQGRMLIDEFNEAFDSDLHMSDVDTMAGYLIT
                     ALGMIPDEGEKLSFDVDNITLVSEEMEGSRILKIRVIFHDPEETEAEPEEERRYFRKE
                     FEDDEPRR"
     gene            complement(41220..42338)
                     /locus_tag="U2K71_001257"
     CDS             complement(41220..42338)
                     /locus_tag="U2K71_001257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM7315352.1"
                     /translation="MFEKLRQSKLMFWSLELLIIAALIMISSEINFIFEPIGTFFSTL
                     FAPVLIAGFLYYLLNPIVKLLMKAKIKRIWAVAIVLLLLVAAIIWILLSVIPSLVQQI
                     SSLASNMPDFIKQVESWLKEVAELPLFKEVDINKYFEQLDISYGTIIQQFLSGVSNSL
                     GSIVGTIASATVVIITAPFILFYMLKDGEKLVPNIQQFFPEKRRNQIVELLGQLNKTL
                     ANYISGQAIECLFVGTFTFLGYMVIGVDYAFLFGVIAGLTNLIPYLGPYLGLAPAFLV
                     TVFNDPVKALLCCVVVLIVQQLDGNIIYPNVIGKSLKIHPLTIIIVLLVAGNIAGLLG
                     IFLGVPFYAICKTIISYIIKIVKEDKQNENKKKIATTS"
     gene            42480..42914
                     /locus_tag="U2K71_001258"
     CDS             42480..42914
                     /locus_tag="U2K71_001258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7315353.1"
                     /translation="MKVVQTKDTMSNIYLDAVRIRHQVFVVEQGVPLSREIDKDEAHC
                     IHFVLYSDKKEPQGTVRLLPLENGKMKLQRMAILSEYRHQGLGKILIEEAENFAKNQG
                     YNTILLGAQSTAETFYEKLGYTAYGDPFEDAGMPHIYMKKTL"
BASE COUNT        12192 a         9214 c         7433 g        14122 t
ORIGIN      
        1 aatttttgtc acaacctttt ttgccgagta aacggtgttc aaaagccagt ttctcggtat
       61 gaagtcaaat ttaagcaaaa atgtgagaag ctattttcac aaaatttttc ttcatactcg
      121 aaactaagcg gctttttcac gacctctttt ttattttgcc atgaacatat cataatacgc
      181 gtccattatt tctttagcaa tcgtcaagtt tatgtggtct tgttcgtcta ataagttcgg
      241 caagacaaca ctgatggcaa tttccggatt gtctgttggt ccgtatgcca cgatattact
      301 attgactgtc gcaatatctg gatgaccttc cgcaaccgtt tctgcagtcc ctgtttttgc
      361 ggaaaggtcc attttcgcac tggctaatgg tttggctgtt gtgtaaggat cggtaccatg
      421 gacaacttgg tggaatcctt cacggaggat cgccatattt tctgcagaaa tattgaccgt
      481 attttctact gttgtgccaa tttctttctt cacttcaccc aagtttccat tttcgtcatt
      541 gccatagatc gctttgacta ggtgcggctg tactcgtttc ccgccattag cgactgttga
      601 agcatattga gctaattgga ttggtgtata tgtgtcaaac tgtccaaatg ccaaatcggt
      661 aaagttacct ggagcatacc attctttgcc attatccgca ttgaacttct tcatataatc
      721 aacagaacgt gagatcccag gagattcatt cggcaaatcg atcccagtcg ttgtaccaag
      781 accaaactct ttgaaagagt ctctcaattg ctgataagct ttagcttctt catctaaact
      841 aggtagtccc attccaggtg tatagtctag tcctaacatc tttaacgcca ctttgatcat
      901 atacgtatta gaagacaatt ccgatgcttt gactgcatca agagcaactt gtccgcttcg
      961 attaaagaca gagctttttt ctgaagcacc ttgcagacga atcggctcat caatcaaaac
     1021 ttgatttccg ctgatcgcgt tacttgccca gcctgctgtc aaagtaccag ccttgactac
     1081 agaccctgga tcaaaggcgc tagtaatcgt gcctaaagca ttctcggtga tttctttgga
     1141 accttgttca tgactgaaac cagtcattgc tagcacttct cctgtctgag gattcatagc
     1201 cactgcataa gcaccaggcg aatactgtgc tttgccgttg ttgatcaatg tttgataatt
     1261 ccttttcaaa atttcttcta cttttgattg gaattctgca ttcatagata acattaaatt
     1321 agaacctttt tccccagaga agatttcttt ttgattcgaa acatttcctt cattatcaag
     1381 ggaaacttca tattgggttt tggttccttg caggacatct tcatattgtt tttccaaata
     1441 actttgtcct acacggtcat tccgagaata accttttgct aggtatttct ctgcttcatc
     1501 tgctggaagt ccttgctttt cagtcgtcac actgccaaga atacttttca gtgaacttgc
     1561 tgcattatat tctcgtgtcc agtctgtgcc agtcgaaact cctggaagtt cagaagcgcg
     1621 ttctgccaca acagctaact cttgatcagt aacaccagag tttttgatat agacagtgtt
     1681 taattcatat gcactattca tccgtttaaa gatggtagca atttttaatt gctcctcgtt
     1741 taaattaatc tcatcagctg tgactttatt gaccatcgca ctatattgtt cgctgttccc
     1801 taattgcttt tcttttgccg aaagtctttt tgtggcttct ttcaaatgtt tgggatcagc
     1861 cagccaaaaa tcttttttat ctctatctgt cagattttca tctaccggaa catcgatcag
     1921 cgagttcagc ttcgttgcga tttcaagcaa atctttagcc gtaacttgtg ttcctctcgt
     1981 atatgtgatg gcctgattcg ctttattcgt aactaaagct tttcctgtgg agtcgtagat
     2041 cattccgcga ggagtacttt cttggaccgt aatcgtagaa gtagacttca ctcgcgcctc
     2101 catctcttcg ccgttcacga tctgcaaata acccaatcta gcaatcaacg caacaaataa
     2161 cgtaaatata atgaaaaaaa gaaaatttaa gcgaaaagga acatgggacc ggcgaaacgt
     2221 tttgttctta ggttcttgtg caccttttct tattttttct aaccaagaag tatttaatat
     2281 ttttttcata aaatctttca tcatttccta tccttccgcc tatttacatt catgcttcat
     2341 tgtacccaaa aacttcttaa taaaaaagaa aaaatgggcg gagcaagaaa ttctttacct
     2401 aatttcaaga taaaatcaac tttttgtttc acagcaatca cttaaagtta catatccaac
     2461 tatttttttc attaaaaaat aaacttattt ttgaaaatta tgttttctta agtcgtgaaa
     2521 cactttgatg gatcatttat ttgtggaaaa ctacgttttg ttattctaaa aaacttatat
     2581 aaaaaatagt aaggttttta tttcgaatcc ataaaatatt cttggattgc ttctcgtgtt
     2641 tttttcactt tttcgctaag cttttcgttt ttagccatat agaaataaaa aatgatatcg
     2701 atcgtagcaa attgggccaa aagtgaatta gttgccgcac ttcgattcgt gatttctttt
     2761 ggtctagatg tttgtacgag tacatcggct atcttgctaa gcgggttatt tccaaattgc
     2821 gtcaacgcaa tgatttcgat attcatgctt ttcgccagct cggctaatgc gactacatct
     2881 gcacttctac ctgagtttga aacaagccaa agcactttct ttttctcatt gctgacaagt
     2941 tgcgggagca agacgtggat atctttttca aaaatgattg gcttaccgac acgagaccat
     3001 ttttgcagaa tatcttctac tactaaagaa gaggcgccaa ccccatataa aaaaacagtc
     3061 tctgccgatt ccaaacactc tacgactttt tggatcgttt cttcttctag aatatctact
     3121 gtttctttta tcgctacttg tgcattgtta ccaagtttgt ttttgataga gtaaaaggac
     3181 tcatttgctt ctatatcact aaaaccttca tcgattccag tctgtagttc tgttgaaatc
     3241 gtgattttga agtccgtcag actttgaaaa ccgatttttt tggaaaaacg tacaactgtc
     3301 ggcgcactca acccggaagc ttctgctaat tcattagctg tcattgtagg gaccttctca
     3361 atattaccga taatgtagtt ggccaacttt ttttctgctg aagaataatc ttccatcata
     3421 ctttcaatac gactaattac gctcataagt tcctccaccc ttttaatgat agtctatgta
     3481 agtataccat atcagaccct tattcacggt cgtatactgg ataaaaaaca aaaaaacaat
     3541 ccgcccaaca gaatatctta tcccatcaat cccacagatt ctattgagca tctgtgtctt
     3601 tgaggaatac ctgttatcta ttgttcggat tgctttctat ctactacttt tttacaggaa
     3661 atttacctat tagttttctc tgctgtaatt tttgacttct gcttccatag catcaataaa
     3721 gatattcaaa tcttctacag ctgtttcttt acttccataa cgacgtacgt tcactgttgc
     3781 atcttccaat tccttgtctc caacaactaa ttggtaaggg atcttttgtg tttgagaggc
     3841 acgtatctta tagcccattt tttcattacg atcatcaact tctacacgca accccttcat
     3901 ttgcaaacgt tctttgattt cataagcata atcgccatgt gcatcaacag aaacagggat
     3961 gattgttgct tgtataggtg ccaaccatgt tggaaaagcg cctttgtata cttctgttaa
     4021 ataagcgaca aaacgttcca ttgtagaaac aattccgcgg tggataacta ctggacgatg
     4081 cgtattttcc ccgtcttctc caacatatgt caaatcaaag cgctctggca ataagaagtc
     4141 taattgtata gttgataatg tttcttctgt accaagagct gttttgactt gaacatctag
     4201 ttttggtccg taaaaggctg cctcgccttc tgcttcaaag taatctagtt ctaattcatc
     4261 catagcggct ttcagcattg cttgggcttt ttcccacatt gcgtcatcat caaaatattt
     4321 atctgtattg tttggatcgc gatagctcaa gcggaaacga taatctgtaa tattgaagtc
     4381 agcatatact gccaccatca attcaagcgt acgtttaaat tcatctttga tttgatcagg
     4441 acgaacaaat gtatgaccat cattcaacgt catttctcgt acacgttgta aacctgacaa
     4501 agcacctgat ttttcatagc gatgcatcat tcctaattca gcgatacgaa ttggtaattc
     4561 acgataagaa tggatcgtgt ttttgtagac catcatgtga tgagggcagt tcattgggcg
     4621 aagaacaagc atttcgccat ctcccatatc cattggcggg aacatgtctt cttgataatg
     4681 atcccagtgt ccagatgttt tgtacaactc aacatctccc attattggtg tatatacatg
     4741 ttggtaacct agactaactt ctttgtctac gatataacgt tcgatcgtgc gtcggattgt
     4801 tgcccctttt ggaagccaga atggcaaacc agaacctact tctggggaaa ccatgaacaa
     4861 gtcaagttct tttcctagtt tacgatggtc tctttctttt gcttcttcac ggagacggat
     4921 atattctttc aaagctttct tgtcaaaaaa ggctgtcccg tatacacgtt gcatcatttg
     4981 attgtttgag tttccacgcc aataagcacc tgccactgat aataatttaa atacttgtat
     5041 acggccagtg gatggaacat gtggtccacg acacaaatcg atgaattcgc cttgttgata
     5101 agcagtaatc acttcttctt ctggcaattc actaatcagt tctactttgt acggatcact
     5161 tgcaaaaatt tctaatgctt cctgttttga aagaacacgg cgttcgatcg gcagattttc
     5221 tttgacgatt ttcatcattt ctgcttcgat tgccggcaaa tcttctgcag ttacttggtt
     5281 ttgtccatta tctgaatcgt agtaaaaccc tgaatcgata gctggtccaa cgccaaagtg
     5341 gatatttgga tagaggcgac gcatggcttg ggccatcaaa tgagctgctg aatgacggac
     5401 taagccaagt gcatcttcat ggtctggtgt gatgatctca atacttgcat cttcctcgat
     5461 tggacgagtc aaatcaacga gttttccgtt cactttacca gctaaagctt tttttgctaa
     5521 gctttttgaa atgctttcag caattgccaa tgttgttgtt ccagactcaa attctttgac
     5581 tgcaccatct ggaaaagtaa tttttatcat aatagtttcc tccttgaaat gttttgattg
     5641 atcagatcat caagctgaaa aacaaaaaaa tcctagcatc tcttgaaaag agatactagg
     5701 acgaaaagtt cgtggttcca cctaatgttc aaacgcaaat gcgttcctca agcgttgtaa
     5761 cggtccgacc gcccttgctt caacaagggt ttcgaaagtg gtcatccttt ggttttctcc
     5821 tggaaaagtt tcagcctagc ttttcctctc ttatcgaaga ctctcaaatt cagttggctt
     5881 tctcatcaat caattatgtg tttatttaac cactaataaa tagaaaaatc aagtgggaaa
     5941 ctaaacttat tttttccatt ccccatttac accgtatgtt ccttttttca tctcattgat
     6001 gatgacatgg atgtgttctt ttggtgcctg cgtgttttta tgaacagctt ccgtgatatc
     6061 tttcatcata ttttctaatt gttccggact gcgaccttct actaattcga catgtacgaa
     6121 tggcataatg ctctctcctc tttctgatcg ttcttttttc aactcgattc tatcatacta
     6181 tgtttgtctt gggtactgaa tcaggaaaaa tggctggatc tttttttgct ctcaaggcac
     6241 tcaaaataga gaccgtatcc acgacttctt gcaaaacagc tccgaataat gcggggatta
     6301 gtcccgtact tgcaattaac atcaaaaaaa tacaaataaa gatgccaatc agtacagact
     6361 gtttcgctat tctcatcgta tctcttgaaa tcactactgc ttttgctaca cgcaagagat
     6421 catctttcaa gatgaccgca tctgcacttt cactagcagc tgtagaacca tgagccccca
     6481 ttgcaatacc gatatctgca gcagcaagtg ctggtgcgtc attcacgccg tctcctacca
     6541 tcgcaacagg tctttgatct ttagggatac tttctaaaat ggtcagttta tcttgaggca
     6601 agctacgggc atgtacagaa tctaattgga ctttttctgc aatcgtttga gcaatggcag
     6661 atccatcccc ggtgatcatc atcaaatttt tcacatcaag ttttcttagc tccttgattg
     6721 tctttgctgc ttctgggcga atctgatccg aaaataggat cgttcctgca aaagatcgat
     6781 caattgttac atagattgct gtattggacg tttctatttc ttcagctgaa acaaaagagg
     6841 cacgtccaat ctttacttca tgattattga ctactgcttg gattccttga ccggtcactt
     6901 ctttcagttg actaacagga tacagttggc tgtttttcac gttgttcact aacgaacgag
     6961 ctaagatatg gctagattcc tgttctgcac tagctgcata cagaagtagc tcttcttttt
     7021 ttattccatt gactggctga atcgcctcta cttccaatat ccctttggta agcgttcctg
     7081 ttttatcaaa agcaatcgtt tttgtacgtg cgagcttttc aatcgttgtt ccagttttta
     7141 caacgatccc gtttttactt gaacggctca ttcctgcaac taaagcaatg ggcgcagcta
     7201 gaatcagtgg acaaggtgat gccactacca gtacctcagc aaatcgtaaa gggtcttttg
     7261 agaaccacca agaaatacca ccgatcaaat aagcaatcaa cgtgaaaggg actgcataac
     7321 gatcagctaa acggacaaat cgggcaggct tttcttttga ttcttcgact agtttaataa
     7381 tcatttgata ctggctgtct gcggcacgtt tttcagcgat catttggatc gatccgtctc
     7441 catttaaaga ccctgacata atctgatctc caggtttctt ggtttccggg cgggactcac
     7501 ctgtcaaaga agattcatcg accgaagatt ctcctttggt cactcgacca tctgcgggta
     7561 caagttcgcc aggcttgact agtaactggt catcaatatt tatttcatcg attactatat
     7621 cctgcaattt tccttctttg aatcgatggg caattcttgg ggtgttatct aacaaagact
     7681 gcagttcttt acccgctcgt ttattcgcgt aatcctctag actatctccg cccgtaagca
     7741 tgatcaatat catcaaacta gcccagtatt ctttgacggc taatgttgca aggattgcgg
     7801 taatcgctaa tacatctacg ccatatcttc ctgaacgtaa cgtcttgatc atttccaaaa
     7861 acatcaagaa ggcgagaatc cctccagtaa atgcgacaat cgcaaatgca agggccggct
     7921 ggtgaaaaac aaattcactt aataaagcta tcccaccaat aagcaaagta agtaaaaatt
     7981 ttttgaaatg tgtcattgtt ttttctcctc ttttatgtga cttagagagg ttagtaataa
     8041 atttccagct gcctctttca tcttaagcat agtccttgac tagcactttt taaaacaata
     8101 ggccttaatt gaaaacaact atcactatca gcgtttccaa aattcatcag ttctacaaac
     8161 aaaaatattc agcaaggatt actttttcga ttcatcagtt aaaaagtacc ttttttcgaa
     8221 aaactatgct atacttttaa ttcgttattt acggcggaag gagcaaacat gcgatgaatg
     8281 agaaacaact agaacaaacg cacgtggatg aaacgatcga actaatccag atcgaacaaa
     8341 atatgctgga tacaaaacaa caagaattga ctcaggaaat gaatcattcg acaaaagaca
     8401 cagctaatca caagattcga gctggttcca atgaagcttt ttatgaatct gctgttgaat
     8461 atcgccaaca tgagcaggaa ttgcttttga aataccatac tcttgaagca cagcaaaaac
     8521 gcctgcaaac gttgtctgtc atgaaagata gtccttattt tgcccgtatc gactttaaag
     8581 aagaccgtga aacagaaaca ttatatctag gtatcgcttc tttaagagat ctggcagaag
     8641 aaacaatcgt tattgactgg cgcgcaccaa ttgccaatct ttattatgaa ggtgagattg
     8701 gtcccgcatt ttatgaaact gaaacagata gaatagatgt cgatctgcag ttgaaacgac
     8761 aatttaaaat ccaagaagga cgtattttat ctatggtaga tacgtcggaa gtaatcaatg
     8821 atgacttttt gctagagata ttggatgaat catcaagcaa ccatatgaaa aacatcgtct
     8881 ctacgataca aaaagcacaa aatgcaatca ttcgggacac aacaagtaaa gtaatgctga
     8941 ttgaaggaat cgccggaagt ggaaagacat ccgctttgct tcaacgtatt gcttttctgt
     9001 tgtatcataa tcgcaagtgg ctcgaggctg aaaacgttct tctattttca cccaaccatt
     9061 tgttttctga ttacatctcg actgtattgc cttcattagg tgaaagcggc gtgcctacac
     9121 agacattcaa aaattatttg gtgcagctgc tgccagagtt ttctttacta gatgaacagc
     9181 aacaagaaag tggttttttg ctaggggaaa aagatccgat acaagtgatg aaaagcggat
     9241 tgaccttagt cgatcagatt gatcgctata tccaaggcat cacaaactac ggtcccttgt
     9301 tccgtgatat gaaaataaat ggccgtacga tcctatcaaa ggaatcgatt cgccaatggt
     9361 ataaagaaac aaatgaacaa ttacctttac atcatagact ttctctatta caaacgaagc
     9421 tcctgaaaaa attaggcggt ctgcaaaaag acgaaacacg tcagcaatgg gtcaaagatc
     9481 tggcggaaga acagctccaa gaactatacg caacagatcc aaatcttgaa tatacagaac
     9541 aaaaggagcg ttcattacgt aaaaaactgg ccaaccaaat cgtacgtaaa agattccgtc
     9601 gaatccatcg agggatcatg aattatcaat ttgtcaatat aacgaaacaa tatctccact
     9661 ttctacaaac tgtgccactt tctcttctaa cacgacataa aatcagcgag accaggtggc
     9721 aggaacattt gacaactgtt cgtacacact tgcgtcaaag agaattgcgt caagaggatg
     9781 ctgtactatt ctttttactg atgcgccgaa catatccagt gacggttaca cacaaagctc
     9841 gctatatctt tattgatgaa atgcaggatt tcgcaccatc acaagtggca ttgctgcaag
     9901 ccttgttccc aaaagcaaat ttgactttct gtggagatct aaatcaaaat gtctttggaa
     9961 atgaaacgat cgttggatca ttggaagacc ttttcgtgga ccgtgaagtc actcgtttcc
    10021 aattaacgac aagctatcgc tccacaaaag aaatcactga ttttgctaat cattttattg
    10081 tgaaagaaca tcaagtagaa acaacagcaa gaaaaggaga ccttcctttg ctcatccaag
    10141 gaacaacaga tgatatcaaa ttgaattggt tagcagatac cattcgaacg actcagggaa
    10201 aagcccggta ttggcgtaca gcaatcatag ctaaaacaca agaagaatgc gaagctcttt
    10261 atgctcaact gccagacgag ttgaaacaac aagtccagct gattttaaat gagagtgact
    10321 ttatgaagcg ttccattatt ttacttccag cctttttagc aaaaggtctg gagtttgacc
    10381 gagttttctt gtgggatgta gatgcagaac atttttcttc tcagcaagac aaattgatcc
    10441 tttacaccat gtgtacacgt gcgatgcatg aattgaccct ttttacctcc aataatgaaa
    10501 gtcctttgtt aaacaatatg gatatttcat cttatgagaa aaaagtactg taagtaaaaa
    10561 gacaactgct atcattaatg ttagcagttg tctttttatt tacaataata attgcagaaa
    10621 aagtaattca atcatttaag gtcatttatt ctatatataa atccaaatct gtttcaggat
    10681 attttaattt gacaagcgaa ataatcattt tcagttcttc catgttttct ggatattctg
    10741 ggtcaaatac cttgctatga cggccaatcc cgccattgtc gatcaatcgg taaattcgat
    10801 tccattcaat gaattggtca attgattgga aattgctgat tggatagccg ccacaatcca
    10861 aaatctctga tacacgaaat gctaggtatg cactatcgat cactttattt tttttcgtgt
    10921 ctatcttctc atttctttcg ataagaattt tttcaggacg atgtttcaag ctccactcta
    10981 atagtagaaa gaaagcctcc atccgatact cttcttcaaa taaccgaaag gctgaatcag
    11041 aacgacgaaa aagcagcgga atcagataat tttgtctata aatcagcatt ggtgcgcggt
    11101 ccaccataca ctctaaataa cggttcagat accctgtacc gacatagttt cctaatcgat
    11161 agccttcaac gccgaatctt gcgtattttt gcgctaattt tgaacaatca gttacgttca
    11221 cagctaactc ttctactttg cggtaaccgc ttaagatacg ttcaaaaggt tctaaagtga
    11281 tttttgcttc catattcttc cccgtccatc ttccacatac ttcatatcat ttcacatgat
    11341 acttatgctc tcatcatacc acaggttgaa ttagaaaaga aaataaatca cacaaaaaag
    11401 caagacaatc tgttagtgag attgtcttgc ttttcgaaaa agactgaata cttttcgtta
    11461 aaactggttg cttagtcttc taatgccaca ttatggaaca catgctgtac atcttctaga
    11521 tcttccagtg catcaatcat tttttcaaat gtttcttgat cttctggaga tagtgttacc
    11581 tcgttttgag ggatcatttg gatctcagcg attgaaaaat cagtgattcc tttttctttt
    11641 aatgcttctt gtatactatg gaaatccgct acttcaccgt aaacgatgat ttgtccttct
    11701 tcttccatta catcgcgaac atcgatgtct ttttccatta ggtattcaag gatctcgtca
    11761 gcgtcttctc ctgcaaaagc aaagattgct gtattgtcaa acatgtaaga aaccgctccg
    11821 gaaacaccca tgttcccgcc atttttccca aatgctgccc gtacgtctgc tgctgttcga
    11881 ttcacattgt tcgtcaatgt atcaacaatc accattgagc cgcttggtcc aaatccttca
    11941 tagcgcaatt cagagtatgt ttcatctcct gcaccttttg ctttttcgat tgctcggtca
    12001 ataatatgtt ttggtacatt ataagtttta gcgcgatcaa tgacgaaacg tagtttttgg
    12061 tttgcatgcg gatcaggatc acctgatttt gctgccgcgt agatttcaat cccaaatttt
    12121 gcataaatcc ggctattatt agcatcttta gcagttttct ttgctacgat atttgcccat
    12181 ttacgtccca tattttcact tcgctttctg atttgatagt ttcataacgc aaaaacgtcc
    12241 tgttccatta tacttgtgaa tcacgattct gtgaagcttt ttttctttct tccttcattc
    12301 ttctcatttt tagtcaatta tctatactcg gattgggatt tgcccgaaat tctgttccgg
    12361 aatattgcct cccaattgat agatttgatc acttctaact aacagttccg cttcttgtgc
    12421 ctcttttttt ccgattcggg aataaataga aatctctttt ttttctttta agaatcttct
    12481 tccagcatcg gtaaatccaa gtagtcgaag ataattttta ttttgttgga caggaatctc
    12541 ttgctttttg atattcagca agacataaca agataatcgt tggatccgtg tccatgtata
    12601 tctttttgtt tttacctttg acacaaaatc ctcaaaacta tcagcagttg cagctgctgt
    12661 tttcatgcgc gattctattc cttcggtcat ctgatagata tctgtcagtt ccagcaaagt
    12721 agaagataac aaccgataac gcaaatacgg ccaataagat tcccaagtct gcagcgaaca
    12781 agttgaaaga tcttcttctg tctgaggcgg cacaagtcca gctattcttt ttccttcttc
    12841 taacgcatgg cgaatggctg ttgcactggc tatcctctga tctgtgatga ttttttcatg
    12901 atagcctgca cttttacgtc ggatcggatg taattccata gggtggacat acttggcatt
    12961 ttcctttgca taacttaatc ccaagatatg atttggcgaa gaaaaatcaa gcgtaatatc
    13021 gggatacaga ctgcgaaaaa cctcagtcat tttttgcgga taactatacg tttgatcaga
    13081 cagttcttga aatgcctctt ctatcctatc ttgattctct tggacaaagc gtccgaaact
    13141 gctgtaatca aacgaagtct cttcttctgt tccaaaacac aaactgtcac agtccagtgt
    13201 ttgcaagatt tttactgcac ctttagcaaa atagtcagct gactgaaccg cccattcgac
    13261 gggaagctcc actactaaat ccacaccatt ttgtaaagct tcattcgcgc gatgccattt
    13321 atcgatgata gccggttcgc ctctttgcaa aaaattcccg ctcattacag caatgacgac
    13381 ctctgctcct gttatttttc gtgccatttc cacatgatag cgatgtccat tgtgaaaagg
    13441 attgtactct acgataatcc cgcagctttt catgcgcatc ctccttcata gttatcgata
    13501 aaaaagaaat agagacaagt aaaaaaaggc aaaaatcgga aaaaataccc gaactatata
    13561 aactattctc gcaaacacaa tacttctgcg acgaaaagct gcactgcaag agcaatgcct
    13621 atatcgttcg gatatataat ttatgttgaa gatttattct tttcttgtta actcgagcag
    13681 gcaaaaccgc tagtctttgt atgctacaaa gaaccagcgc tttgatttct ctgatgctgc
    13741 ttcgtctgta aaatcagaaa atgcttcgac tttcgaaaag ccgctgtttt ccagcattct
    13801 taaataactt tccaatggat aggtacgctc ttgatgtagt tcatcttcac gaataaatcg
    13861 accagaccct tcttgttctt caacgaaaaa ggtaagaaaa tgttcgatgc tgtgtttctc
    13921 gcttcctgga taactatccc acaaaaaagc aaaattatcc ccttgataat gatagctata
    13981 ttctggaaaa acctcatcta cttgataggt ggagtgtacg tcaaataaaa atcggccatt
    14041 ttcttctaac gcttgataga cttcatcaaa cacttgctgt acctcttgtc tatttttcat
    14101 atagcacagc gaatcagaaa aacaggtaat tgcctgatat tgtccgattt ctgataaatc
    14161 gagcatattg ccttccacaa attggatcga tgcgtcttct tcatatgctc gctggctggc
    14221 aatcatcaac atctcttctg aaaggtctaa ccctgtcact ttatatccat cttttgcaaa
    14281 ggcaaccgct aatgctccag taccgcatgc cagttccaaa atattttttg tgtcttcttg
    14341 taaatgacgt ttcgaaaaag ctaaccattg ctgataaagt gtttcgtcca tcacttcatc
    14401 gtaaatgaaa gcaaatgttt cataagccat atgttaatcc aaccattcac taagatcgac
    14461 tagcggagca tctgaccata gtttttccag attatagaaa tttcgttctg gcgcatggaa
    14521 aacatgtacg atcacatctc caagatcgat caatacccat tttccgcctt cttttccttc
    14581 aatacgtttt acttcatatt tgttttcttc ttctttatca atgatttctt ctgtaattgc
    14641 gttgatttga cgttcgctat ttgctgaaca gatcatgaaa taatctgcca acaaggaaac
    14701 atttcgaacg tctaaagcga taatgtcttc tgcccgtttt gaatctgctg cttcaatcgc
    14761 aatttttaat tgttctttac tatctatgat gatttcctcc taatatgagc agcttatttt
    14821 tttgctaccc agtaattata tgtttcgatt gtttttggat aaatctgttg ttcttgcgca
    14881 agcaaatggg ccaatgtatg ttttgtttca aatgctacag cttcgtctag atcagcccat
    14941 gcaatggctc gtgcatcttg taccccagga aaatctcgac ctggttcaat atagtctgct
    15001 acataaatga ttttgtcaag gagactcatc ttagcagccc cagtcgtatg cacccggatt
    15061 gcatgaagaa tctctgcatc ggtgattcct aattcccgct ctacaaacga agcgccaaca
    15121 agaccatgcc agattgcatt attataattc aataactccg gatcaaaacc gtctcttacg
    15181 attaccattt tgaactcctc atctggtcgt tcttttgcat aatcatgtgt caatgcagca
    15241 atactagctt tttcaggaga agcaccgtat tttttggcaa gagccacggc tgtttcttct
    15301 actccaagca catgcttgaa gcgttgttcg ctcattctca tctgtatttt ttgcatcagg
    15361 ttttctctag aataagcagt atattcatta ctatagtcca tattcataca acccttcatt
    15421 ttgaatataa tcaattactt tatctggaac aaggtagcgg atagaacagc cttctttgat
    15481 cttttgacgt attttcgtgg aactgatgtc gatctcaggt acatctaccc aaattacagg
    15541 atatggagta tccgttgtgt atcctggtcg acgtataccc acaaagttga ccatagaagt
    15601 cagctcatcg attttatacc attttggcaa atattctacc atatccccac cgataataaa
    15661 gtaataatct gtatccggat tgttttgcgt caattccttc atagtatcat aggtgtaact
    15721 tttcccacca cgagctaatt caatcgtttc aatctgcaaa aaaggattgt cttccaccgc
    15781 taattccaac atgttgagtc gatgttttgc atcaatcgtc tgtttctcat caacatgagg
    15841 tggctgataa gaaggcatca aaaatacgcg gtcaagacct aaattccggc cagcttgttc
    15901 agccatcact aaatgtgcaa ggtgaacggg attgaatgtt ccgcccaaga tccctacttg
    15961 ctttttcttc tctaagaatt gaggcatttc ttcaggaaat aattgtgaac gaacaaatgt
    16021 cttagcttgc gtattcatca tggtcactcc ttatattgct tttacttctt ttgatagcct
    16081 ttgatacttt tcttttgtgg aaggtttgta taatacaata acacgtccaa tgatttggac
    16141 gatttcgcaa tctatttctt tttctaatac ttgagctgct tcttctgcaa tctcatctgt
    16201 attttgaagt aacgatactt tgatcagttc tcttttttct aaagcttctt caatttgaac
    16261 gatcattgct gaattgatcc caccttttcc gatttgaaaa atcggctgaa gatgatgcgc
    16321 tttgctacgt aaaaaacgct tttgttttcc tcttaaactc ataattttcc ttcttcctta
    16381 aatcaatgct ttgcgtttca atacatctac accctttggc gcccagcccg caattactac
    16441 tggcgctgtt acagtgatcc agcctagacc ggcaaaaacg atgtctgttt tttctttcac
    16501 agaaaactca aaacgaacaa gctccggaaa tgcctcgact tcatcaggac gaggaggctg
    16561 taataatcca cctacatgtt tttgataaaa ttcgtctgct ttttccagtt tcgttcgatg
    16621 aatatttaca tcatttgaca cataagcaat aaatgaagca cgattgccgc tgataaaatc
    16681 aaatcttgca agtccgccca agaaaagtgt ttgcccttca ttcagctgat aaactttagg
    16741 tttgatttct tttaacggtg caatgatccg caaatctttt ttacctaagt agtgcgccat
    16801 ttgatggcga tggataattc ccggtgtatc aatcaaaaaa tgcccgtcat ctaaaggaat
    16861 ctcgatttta tccaaggtcg ttcctggaaa ctgcgaagtt gtgattacat cttgtactcc
    16921 cgctgtattt ttgatgattt gattgataag cgtcgatttt ccgacatttg ttactccgac
    16981 gatatagaca tcccgtccat cgcggtattc ttcgattttt tctaatagtt ccgtcatctc
    17041 atgagatttc ttagcactag ttaataatac atctactgga cgaattcctg cttcgtaagc
    17101 acgttccttc atccattgtg ttaatttagt ttttttcaat gatttaggta atatatccac
    17161 tttgttccca actaaaagta ctggattgtc accgacaaaa cggtgtagtc ctgggattag
    17221 tgaaccattg aagtcaaaaa tatccaccac attgacgatc aatgcatctt tttgccctaa
    17281 ttcattcagt aagcgcaaaa agtcgtcatc cgtcaatgag acatcttggg tctcattata
    17341 atggcgtaat ctgaaacaac gctgacaata gacttccccg gaagctaaac ctttttccaa
    17401 agccgtttgc ggcgtgtatc ctaattcacc gggtttgttt gtttgtataa cggcaccaca
    17461 gccgatacat tgtaattctt ctgtcattcg attccacctc gccagcccat atcagggtgt
    17521 tttgctaata gataggccat aattcttttt tcccaaaaac gattaaattg tgtcttccaa
    17581 ctatctgttg cgataatggg ttttacaagg atgctacgta tccctgcacg attggctcca
    17641 cgtatatcag tcataatctg atcgcctacc attactatct catcatctgc taaatccaat
    17701 tgtttctttg ctatgttgat ccctcttgct aaaggtttca ttgctcgtgc aacatacaaa
    17761 agatcaaatt tttcaatcgc tcgcgcgaca cgttttgaat tattgtttga aacaacaaca
    17821 acagggattc ctgcattttt catttctaaa atccaagtaa gcaactcttc tgttccatct
    17881 ggattattcc atgcaatcaa cgtattgtct aaatcggtca atactgcttt gatgcctaga
    17941 ttctttaatt gggcaggtgt aatctgatag atggcttcta ccatccatgt tggtttgtaa
    18001 tttgaaaaca tgctgtctcc tcatctgcaa aaaatgaagg agcacataaa gtactcctca
    18061 accccgtatt atactgcata tcagcaaaaa aaactacctc tatactcgtt cgtctttcag
    18121 aaaaagcatt tctttacaaa atgaccaaaa ataaagattg atcacagaaa aatttagaaa
    18181 caaaaaacaa ctaatattgg cggaacctct tgtatcgttt ttccagtaat ttatgctgat
    18241 ctaatcgaac aagttccgtc aatctttcaa tcaacgcttt tttcataatg tgtgcgactt
    18301 tatatcgtgg caactgctcg ccgttgaacg tttcaggaat aactcgatca acgattccta
    18361 actcgcgtag ttctggcgca gtaattttca tcaactcagc tgcttctgcg gcgcgactac
    18421 cgtctttcca caaaatcgac gcaaatcctt ctggagaaag cacagcataa atcgtttctt
    18481 ccagcatcca gacttcatcc gctaaaccta gagcaagggc accgccactt ccaccttcac
    18541 caatgatgat ggcgatgatc ggtaccttta aatcagacat ttcgagcaga tttctggcaa
    18601 tagcctctcc ttcgccgcgc tcttctgctc ctacaccgca atatgcacct gctgtattga
    18661 tcaaggtaat caccggtcta ttgaactttt ccgcttgttt catcagtcgt aaagcttttc
    18721 gatagccttc aggatgggga gaaccaaaat ttctgcggat attttcctgc agatcccgtc
    18781 ctttttgaat accgatcact gttaccggtt tgccatctaa tcgagcgatt ccaccaacaa
    18841 tcgcttcgtc atctgcaaat aaacgatcac catggcattc ctcgaaagaa tcgaagatct
    18901 cttcaaaaaa atccaagctt gtccatcgtt ctttagctct tgctaactga acgacatcat
    18961 gtgctgtctt atccacgatc tatccatcct ttctgtgtat gcaattcaag aagcgtatgg
    19021 attttctgtt tcaattccat gcggggaaca atctgatcga caaaaccatg agataataag
    19081 aattcagcct tttggaaatc ttctggaagt tcctgcttga tcgtctgttc gatgacgcgt
    19141 cttccagcaa aaccgatcaa gctttggggt tctgctagaa taatatcgcc ttccattgca
    19201 aaactggcag ttactcctcc ggtcgtcggg tccgttaaaa cagttagata aaaaagacct
    19261 tcattgctat gccgtttgac agcagcagag atttttgcca tctgcattag agaaaagatt
    19321 ccttcttgca tccgtgcgcc acctgaagct gtgaacaaga cgactggaag actttccttt
    19381 gttgctcgtt cgaataatct ggtgatcttt tctccgacaa tcgttcccat acttcccatg
    19441 ataaaattag gatccatcac tccaataatg aagggaatcc cctgaatggt tgctttacca
    19501 gtgaggactg cttcatgaag tcctgtttta tcttgcattt ttttaatttt ttccgtatat
    19561 tcgggaaatg ctagcggatc tttggttaca agatccgtat cccattcttc aaaacttttt
    19621 tcatcaataa tcaaagctag acgctgccat gcaccgattc gaaaagcgta tccgcaatga
    19681 gggcatactt tttcttcacc gatgtctttc gtataaagga tatgtttgca atttggacac
    19741 tttgcccaca tgttatcagg aacagatggc gagttagcag gtttgctctc tctattagga
    19801 ttgatccgaa tatatttctt tttcttaaac agagccatct atgcgcctcc tatctccgtt
    19861 tctggtgtcc aattcggtaa aaattcttct tgtaaaaatg ctgtgctgta atcacctgct
    19921 atgactgcag gatgactaat caaatccatc tgaaattctg cgttcgtgat gattccttct
    19981 gtgataaatt cacttaaggc tcgctgcatc ttcattaatg cttcaaaacg cgtatttcca
    20041 tggacgatta tttttgcaat cattgaatca taatatggcg ggatcgtata tcccgggtaa
    20101 actgcactgt ctactcgcag tcccatccca cctgctggaa gcagtaagtt ttggatcttc
    20161 cctggagaag gagcaaaatg gaaagcagga ttttctgcat taatccggca ttcgattgcg
    20221 tgtccttgga atactacttc ttcttgacac acattcaaag gctcaccggc tgctatctcc
    20281 acctgttttt ttactaaatc gattcctgtg accatttctg ttactggatg ttccacttgg
    20341 attcgagtat tcatttccat gaaataaaaa tcgccttctt catccatcag aaactcgatc
    20401 gttccggcat tttcataatt gactgcttta gctgctcgaa cagcagcgtc tcctagtgct
    20461 tctctttttg tttgtgagat cactacagaa ggagattctt ccagtacttt ttgattgttg
    20521 cgttgtaaag aacaatctcg ctcgcctaaa tgaatgacat gaccaaatcg atcacctaag
    20581 atctgaactt ctatatggcg agcaggataa atgatttttt ccaaatacat atcatcattt
    20641 ccaaatgccg ctgctgcttc ttgctgagct gaagaaaaat gctgtgggag ctcttctttt
    20701 gattgaactt tccgaatgcc tttcccgcca ccacctgcag ctgctttcag catcactgga
    20761 taaccaattc tgtctgctat ttccaatgct tcttctactg tagataatac accagtactt
    20821 cctggaatga ctgggacatt tgcttcttgc atcaattgac gcgcattgat cttatttccc
    20881 attgcatcga tcgttgcact tttaggacca ataaatgtga tattgcactc ttcacacatg
    20941 gaggcaaatc ggctattttc agataaaaaa ccaaatcctg gatgaattgc ttcggctttt
    21001 gtcacgattg cagcgctcag tacttcctga acattcagat atgaatcaac ggcttttgcc
    21061 gggccaatgc agattgcttc atctgctaac tgtgtatgaa gtgcttcggc atctgcttct
    21121 gaatatatcg ctacggtttg gatccctagt tcgcggcatg cacgaatgat acgtacagcg
    21181 atttcaccgc ggttagctat caatacttta gaaaacatat ttcacctatc caatcataaa
    21241 ggttaattcc gcttcggcta cttttttacc gtctacttta gcgattcctt tgcctattcc
    21301 ggctgaagct ttcactttca atatctcaac ttcaaggtat aaggtatctc ccggtgtaac
    21361 tttcttacgg aattttgctt tgtccaatcc gccgaaataa gccgtctttc ccttgaattg
    21421 ttcaagagaa agtaatgcaa ctgctcctgc ttgagccatt gcttcaacaa tcaatacccc
    21481 tggcataaca ggttcttctg gaaaatgtcc ttgaaaaaag ggctcattga tcgttacatt
    21541 tttcttagca acgatccgtt ctccttcaac catttcttcc actcgatcaa tcaaaagcat
    21601 tggataacga tgtggaatga tttcttttat ttcttgtatg ttcattgttt tccctctttc
    21661 ctgctttgct tatttttgct gaatcctaaa gagtggctgt ccatattcga caactgactc
    21721 gttttctaca aggatttctg tgatcactcc gtcggcctca ctagttatct cattcatcag
    21781 tttcatcgct tcaataatgc agataacttc tcctttggca acttgatcac ctactttttt
    21841 gaaagctggt ttgtcgggcg ctggttgcag ataaacgatt cccacgattg gcgaaacaac
    21901 ctcgattcca tcaaatactg ccgattcttt ttctttcatt atttctggag cagttgttgc
    21961 ttgttcaggt atgacattga ctggctccga aactggcaac gtttcgtttg tttgaaccat
    22021 attcatgcct cgacttgttt tatttttatt catatacaat tcaaattgac cttcacgcaa
    22081 atcgaattcc gttagcgagg attgatcaaa ttgactgact aattctctga tttcattgat
    22141 atccaaaatt attcctccca gcgtttcatg cacagaaccg cattatgtcc gccaaaccct
    22201 aatgaattac tcattgcata agtaaaactt gcaggtttgc tttcgttttt gacaactgtc
    22261 aagtcaatct caggatcttg tttttctaca ttgatcgtag gtgggataaa ttgatgttct
    22321 aatgccagca aagttgccaa ggcttcgatc ccgccggctg ctcccaataa atgtccagtc
    22381 atcgatttcg tgctgctgac gtaagtatct ctgaaatttt cacctaatgc gatttggatt
    22441 gcttttgctt ctgcctggtc attggctggt gtacttgttc cgtgagcatt gatatatccg
    22501 acttgttccg gctgtacacc agcttcttcc atcgcaagct tcattgcttt agatgcacca
    22561 gaaccatctg gagtaggagc tgtcatatgg taagcgtcac agtttgcacc gtatccaacg
    22621 atttctccat agattttggc accgcgagct tgtgcatgtt ctaaagattc aagaactaga
    22681 acgcctgctc cttcacccat cacaaaacca ttccgatttt cgtcaaacgg aatcgaacca
    22741 cgattaggat cagttgattc tgagagtgct gttaaagctg caaatccagc aatccccatc
    22801 tcacaaatcg ttgcttctgt tcctccagca agaatgacat cagaatagcc atgtttgatg
    22861 ttacggaatg cttccccaat tgaattcgtt ccgctagcac aagctgttac gatagctgta
    22921 caaatacctt ttgcacctac acgcaaagca atatttccag ctgccatgtt ggcaatcgcc
    22981 attggtacga acattggaga aactcgtttt gcaccttttt catgtagacg aataacttgg
    23041 ttttcgattg ttggaagacc accgatccca gatccgatga tgacgccaaa ccggtcttga
    23101 ttcatttttt ctttatctaa accgctcatc tccattgctt ctaaagcagc gtagattcca
    23161 tagatcgaaa acatatccat tcgtttgcta tcttttttaa taaaatattt atcgtaaggg
    23221 aaatctttga cttcacccgc taaggtaatc cctgtttctg aagcatcaaa tttagtgatc
    23281 ggtgcgatgc cattcgtccc ctcttttaag ctattcaaaa agctgtctgc gtcatttcca
    23341 ataggtgaag tgactccata gccagtaatt accactcgat tcatttgttt gctctccttt
    23401 tcttatccgt gcatgaccaa tccgccatcg acattgatga cttgtccggt gatatatgga
    23461 ttttgtgcta agaaaactgc tgtttgtgca atatcttcta cttgtccgaa acgctgcatc
    23521 ggaatttgct tttccgcttg ttccttgact ttgtctgcta aaacttccgt catatcagtt
    23581 gtgatgaatc ctggcgcaat ggcattacat gtaatgcctc tagttgcagc ttctcgtgcc
    23641 acagattttg ttaatcctac tacgccagct ttactagctg cataatttgc ttgaccaata
    23701 tttccaatca acccagatac actagagaga ttaatgatgg ccccttctcg ctgcttcatc
    23761 atttttttca atacgtgctg cgtcatattg aaggttccaa tcaaattgat atccagacat
    23821 tttttgaaat cttctgcatc cattcgcatc acaagtttgt catttgtgat tccggcattg
    23881 tttactaaca catggatcga tccaagcttt tcttctgctt ctttgatcat ttgtcccgct
    23941 ttctcataat cagagatgtc accggaaact cctacgcatt ttacaccgaa tgcttcgatt
    24001 tcttctatct tttcttttgg aatctctccg cgaccattca aaatgatatt tgctccagct
    24061 tttgcaaaag ctaaggccat cgctttgccg attcctcttg tactacctgt gataaataca
    24121 ttttttcctt ttacatccat aacgttcctc cttatagtat tgctattgtt tcatcaaatg
    24181 tctgcttatt ctcaacacgc agaactggta ttgttttatc gatttttttc ataaatccgc
    24241 ttaatacttt tccggggccg acttcgatga cttgttcgat gccgattgtt tttaacttat
    24301 ggatactctc atagaaacga acaggttgca tgacttgctg ttcaagtagt cccgcgattg
    24361 tttctttctt cattatttcc gttgttgtgt tgcttatgat aggaaaagaa ggttcagaga
    24421 attgaatctg acttaggtcc ttcgccagtt tttttgctgc cggttctaaa atagctgtat
    24481 gaaaaggccc gctgacattt agagggatca ttcttttgaa ccctttttct tttaatagta
    24541 aaacagcttc gtctactgct ttttcttcgc caccaatcac gatctgctga ggtgtattat
    24601 aatttgcagg agatacgatg ccgtacttgc tagcttcatg acagctttcc tcgatcgttt
    24661 caatcggcgc attcatcact gcaaccattt tcccgcttcc agctggggca gcttctgtca
    24721 tataagcgcc tctttttgct actaacgcaa ctgcttcagt gaaggatagc gcattactag
    24781 ctactaatgc cgaatattct cctaaactca gtcctaaagc tgcatctggg atgattccat
    24841 gctccttcaa taagcgataa tacgcgatgc tgactgttag aatagctggc tgtgtatatt
    24901 gcgtctgatc caaacgttca ttttctgtaa agcaaagttc agccatatcg tagccgagaa
    24961 tctcacttgc ttcatcaaat gtctgcttta caacggcttc ttcatataaa tcttttccca
    25021 taccttgata ttgtgcacct tggccactaa ataagaatgc tgttttcatg tttttctcca
    25081 atatatccag aatatatgtt tgattcttcc tattcaaaaa ggccgagaca ttcctatttc
    25141 aggaaatgtc ccagccccca acctaatttt aatgattatg cagtttgttt ttctacgtaa
    25201 gtcactaaat cttgaaccgt tttgattccg tcttctgttt cgattttcac gtcaaattca
    25261 tcttcaattt cattgatgat ttggaataaa tccaagctgt cagcttctaa gtcttcttgg
    25321 atgtcagtag tcaatttgat ttcttcagtt tcttttccta attcttctgc gatgatttct
    25381 tgaatttttt cgaataccat aataatatat tcctccgata ttgtttgctt tttattttta
    25441 taatttaaca gtctacaact ccatgagaat acttccccat gttaaacctc cgccatagcc
    25501 tgtaaatatt actttctgct gttttcctaa ggtgatcttt ccactagaaa ctgcttcatc
    25561 caataagata gggatgcttg cagcagatgt attcccatat ttatccatat tggtcaggaa
    25621 cttttcttgc ggcatcttta cttttcgggc aattttctca ataattctga ggtttgcctg
    25681 atgcaaaagc agataatcaa ctggtttgtc tttcatgact tcttggatat tgtttgcaac
    25741 gtctcgtacg gcaaaatcaa aaatatctct gccagtcatt tgcaaataat acgaaccttc
    25801 tgaatcttct gaatagaacg gatttttatt ttgctggtag ccagcggtca atgagctact
    25861 tctggtaccg tcagctgcta atttttcttt cagcaatttt ttctgtggat tcgcttctaa
    25921 gagaatccca cctgctccat cgccaaacaa cacagcagtc gtacgatcag accaatcgat
    25981 tagcttagat agtgtttcac caccaatcac cagtccctta gttgagccgc agcggatcat
    26041 tttttcagcc atgcttaacg cataaacaaa tcctgagcac gcagcactga tatcaaaagc
    26101 gatagcatga gttgctccca atttcccttg tacttgtgct gctacagatg gtgtcgtgta
    26161 atcgggcgac attgttgcta ctaaaataaa atccaaatct ttagggttta gatttttctg
    26221 ttttatcaat ttttccgcca ctaaaatgca taaatcagaa gtctcctgat ttgttacaca
    26281 tcttcgttca cgaatacccg tccttgacga aatccattca tcagaggtat ccatcatctt
    26341 ggaaagatca ttattagtaa taatcttttc aggaaggacg ctggctgttg cagtaatcat
    26401 tccaaattgt tccattaaat atcctcactt atagtcttgc aagaagtcgt gcaagttttt
    26461 cagtgcatcc agcaaggcat gctgttcctc cgtagccatt ccgtctaaga tgttcttgac
    26521 catttcacga tgaaaatgct gatggacccg aaatagtaac tttccttttt tcgttagtcc
    26581 caactttaca acgcggcggt catcttcact gcggatacgt tcgacatagc ctttttttac
    26641 taacttgtta atagcagtgg tcaaggtccc aacagtaatt gatagttctt tggctacttc
    26701 tgaagttgtt tttttcttgt acatcccgat tgcctcgatc gtgtgcatct ctgtgattga
    26761 cagatctttg aattgagatt ttttcagctc agactcttcg attgtcaaaa tatcattgaa
    26821 gacactaaca agataatcat ttactgtttt taaatctggt tccatgttcc accgtccact
    26881 aattagtttg attatcaaat cttttgatgt tcaaactata tcttaacttt tttcaaaatg
    26941 caaccttaaa agatcatttt tttactatat gagctttttt taagatttta ctcatttaaa
    27001 acaactgata aatcagcatt tataatttct tttattagaa aaatcatcta tctcacatat
    27061 tttgattatc aaagtatttt ctgtgaacag aactatagaa ttccctaaaa cttgtccatt
    27121 atttcaaaat ttgttatagt ctttttattc aaagcgtaaa aggagatcct atgttaaaag
    27181 aaaaaatgaa aactctcccc atgctgccgg gtgtctatct tatgaaagac cgagatggta
    27241 aggtcatcta tgttggtaaa gcaaaacggc tcaaaaatag agtaagcagc tattttcatc
    27301 agaataaaca gcattcaaaa aaagtattac gtatgattca tcatataaca gattttgatt
    27361 tcgtcgtagt agacacggaa ttagatgctt tattgctcga gtgtcaattg atccaacatt
    27421 accgtccttt ttataaccga caaatgaatt atttcagtaa ttataattat gtacatatta
    27481 ccaataaggg atttgtatta acagatactc ctacggctcg aacatacggg ccttttcgtc
    27541 tctataaaaa aatgccgtct attttgcgta taatggaaga aacttatcaa atgccttggt
    27601 tgtctgaaat tagtttgtta gctttgcgag tccagcttcc tgacctacag gaaatgactt
    27661 ttgaacagaa aaaaaaggaa ctccaagggc tgtttcaagg ccgaaataaa aagctactga
    27721 cttacttgaa aaaaagacaa caacatttca tctatcagtt gaatttcgaa aaagccggta
    27781 tgcttcaaaa agatatcgaa ttagttactt attttattag acggatccag gaacaaaagc
    27841 agtttttaag aacaccatcg ctcaccttca gcatgccatt agcagcagat gagagtcaga
    27901 aaaagcacta cctcgtttgt tatggtcaac ttgcagaaac aatgattacc tctggtgacg
    27961 tttctcctga cttctattat gaaaagaaag aagctcattt atctttgaaa cgtcaattgt
    28021 caaaagaaga aatcgatcct gtacaaatct tgatcagtta ccgcaaaaaa ttagaaaaag
    28081 aacagtttga aattgaactg ttaaacaaaa aagaagctga aaagcaacta aattagttgc
    28141 ttctcggctt cttttaggca tcttcaactt caaatgtcaa ttatcctaaa atttattgga
    28201 ttaagtcata gatttccatc gctacgatat cgatattatc aaattcgtat tgctgatgag
    28261 tatctgtttc agttaattca aatgtatcgg tcgctttatc ataagttact gtacacttct
    28321 ctacgccttc tttttcaaaa cggcgaactt gcagttcatt atctgctgac tcgaccatag
    28381 cctccagtcg ttttatgatt gcgactaatt gtgaatgtgt catcggaggt cctccttttc
    28441 ttatttctgt tatattgtat caaaagcata gcctacttgc attgattttc tctaaaaaaa
    28501 ctcgtttttt atgatttttt ttctggtatt tttccttcag gccaccaaag tttgatcaaa
    28561 gcttgggttc cgtcattctc aaaaccttta tctgccaact cttcatatag tttcaatgct
    28621 tgggtagttg ctggcaatac aagatccatt ttttctgctt cttctaatgc aatctttaaa
    28681 tctttgataa aatgtttgac aaaaaaacca ggtgtgtaat cttcttttaa aatacgagga
    28741 gaataattgc ttaaagacca gttagctgca cttccacctg aaagcgtctc aatcactttc
    28801 tttaaatcaa gtccgttttt ttgtgcatag acaagcattt ccgtcattcc tgtcatcgta
    28861 cctgcaatca tgatctgatt agccattttt gtatgctgac ctttcccagc agaaccatgc
    28921 aatgtaaatg tcgtaccaaa ttctttgaat aaaggcagta cttgatcata aacggcttct
    28981 tctccaccga ccatgatcgt cagtgtacca ttctttgctc ctaagtcacc accggatacc
    29041 ggtgcatcta acgcatctgc gcctttttct tttgctgttt ttgcaatttt ttctgccagg
    29101 ctggtgtact tgtagtcaaa tcaactaaaa tatgtcctga gatatctgca gaaaaaatcc
    29161 ccgactgtcc atagtagatt tcttctacat ctttggggta acctaccatt gtaaagagaa
    29221 tattactttg ttctgctacc gcttttggtg tatctgccca gatagctcct tctgttacta
    29281 aaggatcagc ttttgctttt gtccgattat agacagctac ttcgtgtcct gcttctagta
    29341 gatggcgtgc gacagcactt cccatgacgc ctgttccgat aaaaccaagt ttcatttttt
    29401 cgacctccta aaaatcaaaa atccttgctt ctatgctagt atacgctaga aacaaggatt
    29461 ttttcataaa aaatgtttga attttatctt ttaaatgaat ttaattttat tttgattcgt
    29521 tcaatcgagt cttcttcttc ttttcttcgc atctcttctt catgataaac aaccatgtct
    29581 tgataaaatt cgatcatagc ttcgccgaaa tgtgtagatg aaatatcata aagtttagct
    29641 tctaaaactt ttggatcttt aggaacttca ctggaaatat aatcaataag tgctggtgca
    29701 aagtcttcgt cggtttcaaa agttttccct aatgaaggat gatcgaacaa tcggttcaaa
    29761 taagcatttc cttccacgac acaaggtgtt ccggctgcca tcgcttctgt ataagtaagc
    29821 ccttgtgttt ctgaagtaga agcactgaca aaataatctg ccgctttata ataaagtgct
    29881 acatcattgt tgtccacttc tcctgtgaac tgcacaaatt cttctaactg cagttctgag
    29941 atcaattcct ctaactcttc tacataaggt ccttttccta cgataactaa tctggcgtta
    30001 ggataagcgg agatgacctg aggaaaccca ttaatcaagg cttggatatt tttctcataa
    30061 gaaatcctgc ttaaagaaag aatcatcaaa tggttttcct gaagacctag tgactccctt
    30121 aatttgctga tatcctgctg agtgatatca gaacgtttga atttttcaat gtcgatacca
    30181 gtaggaatca cgcgaagcgg tgctattaca ccatagctgc gtaatgtatc aataacacgt
    30241 tcacttggac aaactacacc tgtcgtatga tttgcaaata atctagacaa gtattttaca
    30301 tgggattgcc tgatcacttt tcctttaccg atataatgaa ggtagtcttc atacatcgta
    30361 tggtaagtat ggatcagagg tattttcaac ttcttagcta ccatcttccc aagtatccct
    30421 gctccgaatt ctgtatgggt atgaataaga tctagttcta gttctttggc aaccaaataa
    30481 gcataccaca tgcctctaac aacaactctt cgatccttga aagaaataaa tggtacactt
    30541 ggcattcgaa tcacatcttt ttccaactta tccgcattag gatcggtcgt tgtaaaaata
    30601 tagacttcat gcccatgtct ttctaattct tgttttaatg ttttgatcga tgtagcaact
    30661 ccgctgactt gcggaaagta ggtatcggta aaaaagccga ttttcatgtt tttcgcctcc
    30721 aaactagtcc ttgacctgat ttactttttt taatttgcta gagacttttt cattcagtac
    30781 actttcgtat acatttttaa gttcttcacc gattttattg atacttcgct cttttgctgt
    30841 ttgataaccg gcttgtgtcg tactcggtag ttcttgattg gtaatctgtt ggatcagtcg
    30901 gataaattct tcgtttgtct tccccatata gcagttcttg ctatcttcta accagccatt
    30961 atacactgga atatctcgaa caagtacgtt ttgtttgctt gctaaagctt ctaaaacaac
    31021 gatcccttct gtctcttcat aagaagggaa aaagaataga tctgcacctg aatacgcacc
    31081 ttctatgatc tctccacgaa tgtaaccagg aaacaggaca ttttcaggat gatcttcttt
    31141 tacaattttc cggatgttcc gaggaatcga gtacatggga acatgaccaa accaaataaa
    31201 tgtgtattcg ggcatcttct tagcaatttc tacgaaatca atgattcctt tacgttcgaa
    31261 aaacaaaccg acacaaatga ttactttttg atccggagac agtttgaaat attctctgaa
    31321 cttttgttct ttcattggat cagggtgata tttctctaag tcgatcccat tggaaatagc
    31381 ttgaatcggc acacggatac catagctttc caacaactgt tttgaataag gtgttggcgt
    31441 gatcagatag tccgcttttt ggtacaatcc taccagatat ttttttacga taggagaaag
    31501 ctggttcgat ctgataaagg aatttcggaa atcttcctct gtagaatgcg catgatagac
    31561 aacttttttt ccttctcttc tggctttatt gaccatatta tgactattga ttccgtatgt
    31621 attgatgtgc aggatgtcat aatcttcttt ttcatctagt gtataagaga taccgacttc
    31681 cgtcagagca cgtttttgat gatcaagcgc acgcccgatt ccagattttg caagcatctt
    31741 ttcactttca aaatataata ataccttcac cttacgtccc ccttcactta tatagtataa
    31801 catagcctac aaactgtcac tcaaacttaa gccctatttt tgtctattct tactgacaaa
    31861 aacgttttct taaaaaagaa caaaaaaact ggcgtagctc ttttacaagc cacaccagct
    31921 tttgatacgt tgtttatttt acatattcat gaacaagttc aacgacttct tccatcatat
    31981 cgcattcttt caatgcacga tcagcaagtt cagccatttt cgctgtgtcc aagcgtttca
    32041 ttaaactacg cgttttaaga atagatgtcg cactcattga gaactcatct agacccatac
    32101 ctactagcaa tggaacagct gtttgatctc cagccatttc tccacacata cctgcccatt
    32161 ttccttcagc gtgtgctgca tcaattacat ttttgatcaa acgtaaaatt gatgggttat
    32221 atggttggta caagtaagaa acgcgttcgt tcatacggtc tgctgccatt gtgtattgaa
    32281 tcaaatcgtt cgtacctaca gagaagaaat ctacttcttt tgcaaacttg tctgctaaga
    32341 ctgctgctgc tggaatctcg atcatgatgc cgacttggat cgtatcagat acttccttgc
    32401 cttcacttat caatttttgt ttttcttctt caaagatcgc ttttgctgcg cggaattctt
    32461 tgagtgtagc taccataggg aacatgatac gtaaattacc atgaacagat gcacgaagca
    32521 atgcacgcat ttgtgtacgg aacatttcgt cgccttgttc agataaactg atacgcaatg
    32581 cacgatatcc taagaatgga ttcatttcat gaggtaattg cagatatggt agttctttat
    32641 ctccaccgat gtccattgta cgaacaacta caggttttcc ttccatacct tctaatacag
    32701 ctttgtaagc ttcatattgg tcatcttctg ttgggaagtc tggagaatcc atatacagga
    32761 attcagtacg gtacagacca actgcttctc caccattatt atggacacca actagatctt
    32821 ttggtgttcc gatgtttgca gctaattcaa aatgcttgcc gtcagctgtg acagtttctg
    32881 cattttttaa tttttcccat tctgcttttt gtgctgcgta atctgcacct gctttttcaa
    32941 attcagcttt ttgttcatct gtaggatcga tgattacatc gccttcgatc ccgttgacag
    33001 ccaaaatatc gccttctttg actttagcag taatttcttt tgtcccaacg attgctggaa
    33061 tttctaaaga acgtgccatg atagcagaat gtgaagtacg tccaccaatg tctgttacaa
    33121 acgcttttac aaaattgcga tctaattgtg ctgtatcact tggagtcaag tcatgtgcaa
    33181 caacgaccac ttcttcgttg atcattgatg gatttggtaa agtaacgcct aataagtgcg
    33241 ctaaaatacg tttagcaacg tcacgaatat ctgcagcacg ctcttgcata taagcattat
    33301 cttccattgc ttcgaacata ccgatataca tatccgttac ttcttttagc gctgattcag
    33361 catttacgct gttgtcttta atattttgct tgatttgacc taccatttct ggatctgaaa
    33421 gaaccatcaa atgggcatca aatacttgcg cttctgcttc acctaagctt tgcgctgctt
    33481 tttcacgaat ttgctgcaat tcttcagtag atttagctaa tgcagcatcc aaacgagctt
    33541 cttcagccgc agtatcttct actgttgcct tactgaatgt taaatccggt tgaactagca
    33601 ggtaagcttt tgcaacggct ataccgtcac tagcggcaat cccttttagc atttcaacca
    33661 ttattcagat aagccttctt tcttcattgt ttcaacgatt gctgccattg catcagcttc
    33721 gtcagcacct tctgcagtaa tagtaacatc agaaccttga cctacaccta aagacataac
    33781 gcccatgatt gatttcaagt ttactgattt acctttatac tctaagttta catcagagtt
    33841 aaatttgctt gctgtttgta ctaatagtgt agctggacga gcgtggatcc cagtttctgc
    33901 tactacgtga aattctttct tttccatatt aatcggtctc ctttaagtga gtgttgtttt
    33961 tatgtgaggg ttttcttttt tagtaattca gagaatccta acaaatgcca ccctttcatc
    34021 tgtaaagatt aacatttttt ttcgttagat acaacagttt tcattcaatt tcagataaaa
    34081 gtattcaaat tattccggat tctttttcat ccttttcttc acattttata acaaacgtgt
    34141 ttatttcctt tttcttgtcg ctttctcttc tcctgacgtt tatcattctt tcctttgacc
    34201 ctctttgttt tcatatgaaa cgaattactt gcttcccttt ttatttttag tataataaga
    34261 gtcaaaggtc aggaatggtc aatagagatt tcaccttttc tacctcataa atctcttaaa
    34321 aaactagggg ttattctttt acctcatgaa aaacgagatg taaaatatgg atgtacccat
    34381 ttttgtgaaa ggacgtgaat gattcatgct ttgtcaaaac tgcggcaaga atgaggcaac
    34441 gattcacttg tatgccactg tcaatggtca gcggacacaa ctcgattact gtcagagctg
    34501 ttatcaaaaa atcaaaaatc aacaaaatgg aggtctgact aatatggccc aaaatgatcc
    34561 atttggattt ggcagtttgg acgatctttt ccgttccatg tctcgccaaa tgcaacagca
    34621 aggaaattat gagcaaacac caccaacaca atttggcgga aacaacaact tcaatggtgg
    34681 tcagccacct caaggcaata cagaaggttt attgggagaa tacggaatca atatcacaga
    34741 acaagcacga caaggtgata tcgatcctgt gatcggacgc gatgacgaaa tcaaacgagt
    34801 cattgaaatt ttaaatcgcc gaaccaagaa caatcctgtc ttgattggag aaccaggtgt
    34861 tggtaaaaca gcagttgttg aaggtttagc acaaaaaatc gtagatggtg atgtaccgca
    34921 aaaattaatg gacaaagaag tcattcgttt agatgtcgta tctttagtcc aaggtactgg
    34981 tatacgagga caatttgaag aacgtatgca aaaattgatt gaagagatcc gtcaagcaga
    35041 aaatgtgatc ctattcatag atgaagttca cgaaatcgtc ggtgctggtt ctgctggtga
    35101 tggaaatatg gatgctggta atattttaaa accagcttta gctcgaggag aattgcaaat
    35161 ggttggtgct actaccttaa atgagtaccg catcatcgaa aaagacgctg ctttagaacg
    35221 tcgtatgcaa cctgtacggg tagatgaacc aactgtcgaa gaaacgatca gtattttgaa
    35281 aggtttgcaa aaacgttatg aagactatca tcatgtaaaa tatactgatg aggccatcga
    35341 agcagccgca actttatcca atcgttacgt ccaagatcgc ttcttgccgg ataaagccat
    35401 cgatttgcta gatgaaacag gttctaagaa aaacttgacg atccaaatcg ttgatcctaa
    35461 aacaatcgag aaaaaactgc aagaagcaga agaacaaaaa gttttagctt ctcgagaaga
    35521 agatttcgaa aaagcagctt attatcgcga tcagatcaat aaacttcaaa aaatgaaaga
    35581 acgtcagctc actgaagagg aaacacctgt gattaccgaa aaagatatgg aaaaaatcgt
    35641 tgaacaacgg acaggtattc cagtgggtga attaaaagaa aaagaacaga cacaactgaa
    35701 aaacttagca gatgacttga aagctcatgt catcggacaa gataacgctg tagatcgagt
    35761 agcgaaagcg attcgccgaa accgtgttgg attaaacaaa cagaatcgtc caatcggctc
    35821 cttcttattt gttggaccaa ctggtgtagg taaaacagag cttgcgaaac aacttgccta
    35881 cgaactattc ggttcacaag attcaatgat tcgtttcgat atgtctgaat atatggaaaa
    35941 acatagtgtc tctaaattga ttggttctcc tccaggttat gtcggatacg aagaagctgg
    36001 tcaattaaca gaaaaagtac ggagaaatcc ttacagtctt gtcttgttag atgaagtaga
    36061 aaaagctcat ccagatgttt tgcatatgtt cttgcaaatc ttagatgatg gtcgattgac
    36121 agatgcacaa ggaagaacgg tcagctttaa agatacgatc atcattatga caagtaatgc
    36181 cggaactggt aaagtagaag caaacgttgg ttttggtgcg gcacgcgaag gtgttactcg
    36241 ttctgtattg aaccaattaa acaactactt tacaccagaa tttctgaacc gttttgacgg
    36301 gatcatcgaa ttcagtgcat tatcaaaaga aaacttgatg acaatcgtca cactaatgct
    36361 agacgatgtc aaccaaatgc tggcagcaca gcaacttcac atcgaagttc ctacgaatgt
    36421 caaagaaaaa ttagttgact taggctatga tccatctatg ggtgctcgtc ctcttcgacg
    36481 cacgatccaa gaacaaatcg aagatggtat cgctgaattt tatcttgatc acccttctat
    36541 ccatgagttg aaagcaaaac tggataaaga tggaaaaatc atcgttactt ctaaaccaga
    36601 acgtttggct aaagaatcag ccgaagaaac ggctgaataa atccagtttt atagcgtgag
    36661 acggtatttc tttgagaaat gccgtctcat tcttttttca gaaaagtcaa aatactgttc
    36721 attttataaa aaagaaatta taataagaga aacaagcaat taaaaataca taaaagaggt
    36781 gagcgccatg aaattggtaa atgtaacaaa cagccattcc cgtcttgtat taaaccagct
    36841 tgaaaacact gatgcccatc tagtcaaggt ctacacagcc ggaaatacca ctattgtcta
    36901 cactgaagcc cctgaacata atgaaattct gctgatcaat gacaaacgta aaatccagcc
    36961 gaaagaaatc gaagatgcta aaaattattt cttagataaa atcaaagacg ccgtttacca
    37021 cgaggatgaa atcaaagtaa tcgaattggc aggccttgtt gaaatctcga ttccaaaaca
    37081 tgaattaggc gctacattag ccgtgtaaac aaattttgaa cataataatt aaagagcgtg
    37141 ggacaaaagt aaagaacact tttgtctcac gctctaaaca cgtataaacg gcgaaagcag
    37201 aagcaactcc ttcggaaata agctgaaatt cacgaaaatt gcttttcaaa tttttgtgaa
    37261 ttccagctta tttcttggag ttaaacgctt ctgtctcagc ctctttttct ttctagcttt
    37321 ttgatttttc tcttttcttt tgtacttttc tcttttcatt ttcagttcta cgcgtgtctt
    37381 tatttttaac tttttcctct ttatggatga ttgatcgtac tttttgtgca atttgctcgt
    37441 ttttgtccca gtcttttaca tctaataatc gacataatcg taacatctct tcttttttct
    37501 gtttttttct ttcacgtctt tgccattctg tttccatgtg tcacactctc ctttctttaa
    37561 tcatatgtac gcaaaataat ttttatattt ttttgattag acatttctat gattttaata
    37621 tagtaaaaag agccgtcacg gaaaatccgt cacggctcta aacaaaatat gagaggctgt
    37681 ttttgtttgg ctattcttat tacttgatgc agggcagctt gtgtcaagac ttctttacat
    37741 caaacttttt aattttacat tgggatattt atccgcaaac cagcgttctg caaattggtt
    37801 ctcgaataag aacaacggtt ggtcgaaacg gtcacgagcc aagatattgc gacttgaact
    37861 catcttctca tccaaatctt ctggatcgat ccagcgagcg attttgtgtc ccattggaga
    37921 catgattact tcggcgttat actcatttaa catacgatat tggaatactt cgaattgtag
    37981 ctgaccaaca gctccaatga tatattcttc cgttagatat gttttataaa gctgaatcgc
    38041 tccttcttga actaattgat agattccttt gtggaaggat ttttgtttca tgacattttt
    38101 tgcagtcact ttcataaaca gctctggagt aaatgaaggg agttcttcat aagcaacttt
    38161 tagttttcct tcgaacaatg tatccccaat ctgataattg cctgtatcat atacaccgat
    38221 gatatcgcct gctactgctt tttcgacgtt ttctctcgaa tctgccataa attgtgtgac
    38281 attgcttaat ttcagttttt tatttgtacg ttccaaccag acatccattc ctcgttcgaa
    38341 tgttccagaa cagattcgga caaaagcgat tcggtcacga tgggctggat tcatatttgc
    38401 ttggatcttg aatacgaaac ctgaaaattc ttcctcataa ggacttacca tctgatcatc
    38461 ttgtgttttg tgtccataag gagctggtgc caattcaacg aaagtctcta aaaaagtctg
    38521 tacaccgaag ttagtcaatg cagaaccaaa aaatactggt gtctgatcgc cacgagcaat
    38581 tttttcttga ttaaatgcat ctccagcctc tttgattagt tctacttctt ctaagacttg
    38641 ttcatataag gaattttcag ttagaggatg attttcggga agcagtccat cttcatccaa
    38701 ttctactaat cgttcatcct gataattttc cggacgataa aactctacac gattatggta
    38761 gatatcataa agaccttcta gccctttacc cataccgatt ggccaattca tcggataaga
    38821 ttcgatatct aatagctctt ctaattcttc taacaattcc aatggttcac gaccatcacg
    38881 atctagtttg ttgataaaag taaatatcgg gatgccacgt ttttttacaa cttggaataa
    38941 tttctttgtc tgtgcttcga tcccttttgc actatcgatt accatgaccg cactatccac
    39001 ggccatcaaa gtacgataag tatcttcaga aaaatcttca tgtcctggtg tatccaagat
    39061 atttactcgt ttcccttgat aatcaaactg cataacagaa ctagtaacag aaatcccacg
    39121 ttgtttctcg atttccatcc agtctgattt ggcaaaatta cctgtttttt tgccttttac
    39181 ggtcccggct tgacggatag ccccaccaaa caatagtaat tgttctgtaa tcgttgtttt
    39241 cccagcatcc ggatgggaaa tgatcgcaaa agttctgcga ttgtccactt gttgctttaa
    39301 ttttggatta ttcataacct aactccattt cttccattca attatttata ttttctctag
    39361 gcacagattg tccgatctct taaagaaaga caatttcata tagggctgtg acaaaagccg
    39421 tgtagcagtc tctttttccg agtaaacggc gttctagaga cagcctatca gcaataagcc
    39481 cacccaagcc aaaaatacga aaagctgttt ttgcttggct gctcttatta cttaatgcag
    39541 gatagctttt tcacaacctt tgtcaactac tacaactgaa tcagtatagc atggattcag
    39601 cgaatgaaaa agccaccaaa ccataagagt caaatatttt tttgtcagct gactaaaaga
    39661 aaagacgtgt tgcttgttta ttcaagcaac acgtctttca taaccaagtt tccaattatc
    39721 ttcttggctc gtcatcttcg aattcttttc tgaagtatct tcgttcttct tcaggttccg
    39781 cttctgtttc ttccggatca tgaaaaatga cacggatttt aaggattcga gaaccttcca
    39841 tttcttcaga aaccagcgta atattatcga catcaaacga taacttttcg ccttcatccg
    39901 gaatcatgcc taatgctgta atcaaataac cagccatcgt atctacatca ctcatatgca
    39961 gatcactgtc aaaagcttca ttgaactcat caatcagcat acgtccttgg atgatatatt
    40021 cgtgttcatc gattttttca taaagattct caacttcatc tgattcatca tcgatttctc
    40081 cgacgatttc ttctaaaagg tcttctagtg ttgctaaccc aacgacgccg ccatattcat
    40141 ctaataaaat agccatctga ttctgcgtct ttttcatttc atataacaga tcatcaataa
    40201 agaccgtttc aggaacgaat aaaggctcct gcatgatttt cttgatatct aaattatcaa
    40261 agccgaattt atgggcagct tttagtaaat tctttgtatg aaggattccg actactttat
    40321 ctttgtcttc gttataaaca gggattcttg aatagttttc ggataaaact tcattcacat
    40381 tttcttccac agtgtcgttg atatcaatca taaatgcatc cgttcgtggg accatgactt
    40441 ctcgggcaac tttcgtatca agagaaaaaa ccccttggag catttctaat tcttcattat
    40501 tcaatacgcc ttcgttttca agcatataac gcatttcatc tctggtcatt tttgaatctt
    40561 catcatcaaa agtcatcggc gtgatacgcg ccaataaact tgtggaagca gaaagcagcc
    40621 agacgaaggg cttagctact acaccaatca agcgtatcag ccctgaagtg aactttgcga
    40681 cttcttctga tttatttaaa gcaatacgtt tagggtacaa ttctccaaaa acaatcgata
    40741 cataggtcaa aatagccagc acaatgatat ttgcaatatt ttttgccgct gtgccgccac
    40801 ctaataccgg agctaaccgt tccgataaag tattagctag tgaagcacca gacaaaatgt
    40861 tgaccaaagt gattcctact tggattgtcg ataggaaatt atttggattc tgcaatattt
    40921 ttaaaagctt ttgagatttc acatctcctt catcagcttt ttgctcgatt cgattcttat
    40981 tgatcgaaac aacagcaatt tcggaagctg ctaaaaaagc attgatcaat gtcaaaacga
    41041 tcaataataa aatctgcgct aacagcgact gactctcagg gtcagcattc ataatcgcat
    41101 tctctcctta taaaattaat ttttagttta cgatagaaaa aaacgtggca ctatagccac
    41161 gttaaaatgg tatcacaaag caattttctt tgcaataaaa acaagtgtac taaaactacc
    41221 tacgaggtcg tagcaatttt cttcttgttt tcattttgct tgtcttcttt gacgattttg
    41281 attatatagg aaatgatcgt cttgcagatt gcgtaaaaag gaacacctaa gaagatacca
    41341 agcaatccgg caatatttcc agctactaga agaacgataa tgatcgttaa tggatggatt
    41401 ttcagtgatt tcccaattac attaggataa atgatattcc catccaactg ttggacgatt
    41461 agcacaacca cacaacaaag taatgctttt accggatcat tgaagactgt tactagaaat
    41521 gcaggtgcta gtcctagata aggtcctaaa tatggaatta ggtttgtcaa tcccgcaatg
    41581 accccaaata agaaagcata atccacacca attaccatat atcctaaaaa tgtgaaggta
    41641 ccaacgaata aacattcgat tgcttgtccg ctaatgtaat tagccaatgt tttattcagt
    41701 tgccccaata actccacaat ttggtttctt cttttttctg ggaaaaactg ttggatgttt
    41761 ggcactagtt tttctccgtc tttcaacata tagaacaaga tgaatggtgc tgtaatgatt
    41821 actacggttg ccgaagcgat cgttccaacg atcgaaccta aactattgga tactccacta
    41881 aggaattgct gaatgattgt cccgtaagaa atgtctaatt gctcaaaata tttattgata
    41941 tctacttcct tgaacaaagg caattcggct acctctttta accagctttc tacctgtttg
    42001 atgaaatcag gcatattaga tgccaatgag gaaatttgct gtactagact tgggatgaca
    42061 ctaagtaaaa tccaaataat tgctgcaact aaaaggagca gcacaattgc gacagcccat
    42121 attcttttga tttttgcttt catcaacagc ttgacaatag gattcaataa atagtaaaga
    42181 aagcctgcaa tcagtacagg tgcaaataac gtggaaaaaa atgtcccgat cggttcgaaa
    42241 ataaagttta tttctgatga aatcatgatc aatgcagcta tgatcaataa ttcaagcgac
    42301 caaaacatta gtttagactg tctaagtttt tcaaacaata gatttcctcc tttctttctt
    42361 actagtatat attatattcg ttaaaaattc ttttattctt tttttatttt tgaaaacttg
    42421 ccatactttt tatttgtttt attttccagt atactatacg aaaggaaggt ggctagacca
    42481 tgaaagttgt acaaacaaaa gatacaatga gcaatattta cttagatgct gtacggatca
    42541 ggcatcaagt ttttgtagtc gaacagggtg tgcctttgtc tcgagaaata gacaaagatg
    42601 aagcccattg tatccacttc gtcctgtact cagataaaaa agaaccgcaa ggaactgtac
    42661 gtcttttgcc tttagaaaac ggaaaaatga aattacagcg gatggcgatt ctttctgaat
    42721 acagacacca agggttagga aaaatattga ttgaagaagc agaaaacttt gctaagaatc
    42781 aaggctataa tacgatttta ttaggggcac agtccacagc tgaaactttt tacgaaaaac
    42841 taggatatac agcttacggc gatccttttg aagatgctgg catgcctcat atttatatga
    42901 aaaagacttt ataaaaattt atttcgaaaa aagcaaaaaa cagcaagctt ttgagaaaaa
    42961 t
//
LOCUS       DASHHB010000022        38396 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.022, whole genome shotgun sequence.
ACCESSION   DASHHB010000022 DASHHB010000000
VERSION     DASHHB010000022.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38396)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38396)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38396
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.022"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..555)
                     /locus_tag="U2K71_001259"
     CDS             complement(<1..555)
                     /locus_tag="U2K71_001259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEM7315354.1"
                     /translation="MAIEAGLVLFFVFVVLLVLGMPIAISIAFSSMMTLLLVIPFDVS
                     AFSSAQKMVGSINSFSLIAIPFFVLSGIIMNNGGIAKKLVDFAMLFVGRVPGALAHTN
                     VLGNALFGSISSSAIAASTAIGGVLIPQQVEEGYDRKFATAVNIASAPTGMVIPPSTA
                     FIMYSLVAGGASISALFMGGYLVGA"
     gene            complement(571..1050)
                     /locus_tag="U2K71_001260"
     CDS             complement(571..1050)
                     /locus_tag="U2K71_001260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter small permease"
                     /protein_id="HEM7315355.1"
                     /translation="MKMILNKVLEILGAVLLVGMVAVVLWQVFSRTVLGNPNTVTEEL
                     VRFGLVWFAMLSSAYVVGQKARLAVTVLSEKLTGKKSKILEVVVQSLFLVFAAMIMVY
                     GGWNAVTLTMGQISPSLSIPMGYVYLSVPVSGVLIIIYSLINLIDTLSNKPAIQEKI"
     gene            complement(1056..2080)
                     /locus_tag="U2K71_001261"
                     /pseudo
     CDS             complement(1056..2080)
                     /locus_tag="U2K71_001261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294179.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="TRAP transporter substrate-binding protein"
     gene            complement(2097..3203)
                     /locus_tag="U2K71_001262"
     CDS             complement(2097..3203)
                     /locus_tag="U2K71_001262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEM7315356.1"
                     /translation="MIIKEEIFVPKELVKEKIDLLVENLTTIKDDNGEFLLDFDGLKV
                     DDKSWTVWNWPQGVGLYGIYKNYQMTKNPRAYQVVNEWFEDRMEEGAPPKNVNTMAPL
                     LTMAYLYEDTKDSRYLPYLEQWAEWVMNDMPRTNEDGLQHATYGPENKNQLWDDTLMM
                     TVLPLAKIGKLLNRPEYLEEARYQFMIHIKYLMDKRTGLWYHGWTFEGNHNYAEAFWA
                     RGNCWLTIAIPEIIEILELSKEDALRKLLIETLEAQVRALKTYQSESGLWHTLLDDPS
                     SYVESSATAGFAYGILKAVHKRYLPQEYKEVAYRAIQGLLEQIDEKGEVQNVSIGTGM
                     GDSLDFYRNIGITAMPYGQSLTVLCLTELLVSYC"
     gene            complement(3432..6029)
                     /gene="adhE"
                     /locus_tag="U2K71_001263"
                     /gene_synonym="adhC"
     CDS             complement(3432..6029)
                     /gene="adhE"
                     /locus_tag="U2K71_001263"
                     /gene_synonym="adhC"
                     /EC_number="1.1.1.1"
                     /EC_number="1.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional acetaldehyde-CoA/alcohol
                     dehydrogenase"
                     /protein_id="HEM7315357.1"
                     /translation="MAKVIEKEKTKTIDVQAMIDDLAEKANAALKEMESFDQEKVDHI
                     VHEMAMAALDQHMPLAKMAVEETGRGVYEDKAIKNMYASEYIWHNIKHDKTVGVINED
                     VQKGLIEIAEPVGVVCGVTPTTNPTSTTIFKSMIALKTRNPIVFAFHPSAQKSSAEAA
                     RVVRDAAIAAGAPENCIQWIEHPSIEATSMLMNHPGIAIVLATGGAGMVKSAYSTGKP
                     ALGVGPGNVPAYIEKTAKIKRAVNDLIVSKTFDNGMICASEQAVIVDKEIYAAVKAEF
                     QAHQVYIVKPDELQKLEDAVMNEGKYAVNPSIVGHSAMEIAKLAGISVPKGTKMLIAE
                     LEGVGPDYPLSREKLSPVLAMIKANNTDHAFDLCEGMLNLGGLGHTAVIHSENEELHV
                     KFGLRMKACRILVNTPSAEGGIGDIYNEMIPSLTLGCGSYGKNSVSRNVSAVNLINVK
                     TVAKRRNNMQWFKLPPKIFFEKNSLLYLEKMENVERVMIVCDPGMVQFGYCDTVREVL
                     ARRKNDVKIEVFSDVEPNPSTNTVYAGTKLMADFKPDTVIALGGGSAMDAAKGMWMFY
                     EHPDTSFFGAKQKFLDIRKRTYKIDKPEKTQFVCIPTTSGTGSEVTPFAVITDSETHV
                     KYPLADYALTPDVAIVDPQFVMSVPASVTADTGMDVLTHAIESYVSVMASDYTRGLSL
                     QAIKLVFDYLEKSVKTPDMESREKMHNASTMAGMAFANAFLGICHSVAHKIGGEYGIP
                     HGRTNAILLPHIIRYNAKDPQKHAMFPKYDYFRADTDYADIAKFLGLKGETTEELVEA
                     LATAVYELGKSVGINMSLKAQGVTQETLDTTVDRMAELAYEDQCTTANPKEPLISELK
                     QIIIDAYNG"
     gene            complement(6334..6714)
                     /gene="yajC"
                     /locus_tag="U2K71_001264"
     CDS             complement(6334..6714)
                     /gene="yajC"
                     /locus_tag="U2K71_001264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HEM7315358.1"
                     /translation="MGTLPMLVMFIALLAMWFFMSRSQKKQQQERQNLLDSMKPGDSV
                     VTIGGLHGVVSEIDNDKRTVVLDCEGIFLEYDRAAIKTVKPGTVVTNDATTTVETTEE
                     PAETVEVKTETTETLEETEEKEEK"
     gene            complement(6811..7956)
                     /gene="tgt"
                     /locus_tag="U2K71_001265"
     CDS             complement(6811..7956)
                     /gene="tgt"
                     /locus_tag="U2K71_001265"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HEM7315359.1"
                     /translation="MTQPAIRYRLIKKEKHTGARLGELITPHGTFPTPMFMPVGTLAT
                     VKTMSPEELKEMGAGVILSNTYHLWLRPGEDLVEEAGGLHKFMNWDQPILTDSGGFQV
                     FSLSDMRNIEEEGVHFKNHLNGSRMFLSPEKAINIQNKLGSDIMMSFDECPPFDESYD
                     YVKKSVERTSRWAERGLKAHANPDRQGLFGIIQGAGFEDLRRQSAKDLVGMDFPGYSI
                     GGLSVGEPKSEMNRVLEFTTPLIPENKPRYLMGVGAADSLIDGVIRGVDMFDCVLPTR
                     IARNGTCMTSQGRLVVKNAKYAHDFRPIDEKCDCYTCKNYTRAYIRHLIKCDETFGIR
                     LTSYHNLYFLLNLMKQVRQAIMDDNLLEFREAFFEEYGFNKENAKSF"
     gene            8174..8872
                     /locus_tag="U2K71_001266"
     CDS             8174..8872
                     /locus_tag="U2K71_001266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF975 family protein"
                     /protein_id="HEM7315360.1"
                     /translation="MQSSAELKRQAKESLKGRWGQAVLLNLIPTLIGIATILLIMIPI
                     ILFATTIYDGSASVSEATTSGGGSSGGGGGIISSIISALFMSGISWTYLDILRGQKDQ
                     IEPFKDAFRGFKGLFIGGVISLAVLISIFTTLWGLLLVIPGIIKSYSYSQSYFIYYDI
                     VQETGQTPKVLDTITASRKLMDGYKGQLFWLDLSFIGWHILALATAGIGYLWLSPYIS
                     ATKAAFYEQLPKDI"
     gene            complement(9040..11679)
                     /locus_tag="U2K71_001267"
     CDS             complement(9040..11679)
                     /locus_tag="U2K71_001267"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM7315361.1"
                     /translation="MEEYKQTIEEVKKVTEADPDTGLSSQEVDERRKKQGLNKFDEAP
                     KESMIKKFFRSLSDFTTIILLVAAVISFYTAFATEHGDLFEGLLIIAIVVINSVLAIV
                     QEGNAEKALESLQDMNKQTATVIRDGKVTTVESEQLVVGDILVLESGDAISADARLIE
                     ASQLRVEESALTGESEAVEKDAAFIAEEDESLGDQLNMVFKGCTVAAGRGKAIVTAIG
                     MATEMGKIADLLNENTMQKTPLQVRLNQLGKRISMIALAAAALVFVIGELQGEPLLEM
                     FMTSISLAVAAVPETLTVIVTLTLAYGVQKMARKHAIIRQLPAVETLGTANVICSDKT
                     GTLTQNEMRVRRVWSKEDEVTNIEDSMTNSAMEILKMAALCTDVTVEQEDDDLVIKGN
                     PTEVAIVRAVEENYHTKAELEEKYPRVNELPFDSERKMMTTVHQMGEKYISITKGAFD
                     VLAPRFSSGDVEQAAIVNDRFGKRALRVIAVGYATYDEEPQDISSEALEKDLRLIGLI
                     GMIDPPRPESKGAIKRAKKAGIKTVMITGDHVVTASAIAKELGILNDPSEALSGSELH
                     QLSDEELDARVKALSVYARVTPEDKIRIVKSWQRTGAVVAMTGDGVNDAPALKASNVG
                     CAMGITGTDVAQSAADMILTDDNFATIVDAVSQGRSVYQNIRKAINFLLSCNISEIFI
                     VLIAMLLGWGAPFTAVQLLFVNVVADGLPGFALGREPAEHGIMDQPPIPKNEGIFARG
                     LLQKIAINAGVFTIVTLFGYYLGSYVDTISPWVDASQHVGQTVAFLILAYSSILHVFN
                     VRSSQSIFKVNLATNKALVEMALLALAITTAVALLPFTQELFGLVHISLNHWFLVGIL
                     SIVPIAVNELIKFHRLPEAEEEE"
     gene            complement(12298..13329)
                     /gene="queA"
                     /locus_tag="U2K71_001268"
     CDS             complement(12298..13329)
                     /gene="queA"
                     /locus_tag="U2K71_001268"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HEM7315362.1"
                     /translation="MLTTEDFDFDLPEELIAQTPLKDRDHSRLLVLDRKTGEMEDKHF
                     HEIIEELQPGDALVMNNTRVLPARLYGEKPDTGAHLEVLLLTNTQGDTWETLIKPAKR
                     AKVGTRISFGDGRLQAVVKEELEHGGRIIDFEYEGIFLEILESLGEMPLPPYIKERLE
                     DPDRYQTVYAKENGSAAAPTAGLHFTKELLDEIEAKGVELVYLTLHVGLGTFRPVSVD
                     KIEEHHMHSEFYRLTEEAAERLNQVRKNGGKIVAVGTTSIRTLETIGTKFNGEIKADS
                     GWTDIFITPGYEFKVVEAFSTNFHLPKSTLVMLVSAFAGRELTLSAYQHAIEERYRFF
                     SFGDAMFVK"
     gene            13522..14697
                     /locus_tag="U2K71_001269"
     CDS             13522..14697
                     /locus_tag="U2K71_001269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628506.1"
                     /note="Members of the family are the ATP-binding subunit
                     of ABC transporters for substrates such as betaine,
                     L-proline or other amino acids, choline, carnitine, etc.
                     The substrate specificity is best determined from the
                     substrate-binding subunit, rather than this subunit, as it
                     interacts with the permease subunit and not with substrate
                     directly; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine/proline/choline family ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM7315363.1"
                     /translation="MIEFQNVSKIYKGGKVAVEDVNLSFEKGEFICFIGTSGSGKTTT
                     MRMINRMTDPTQGKILIDGKSIQEIDPVELRRQIGYVIQSIGLMPHMTIRENITLVQK
                     LLKVSQEERNKTAEKMIDLVELPREMLDRYPHELSGGQQQRIGVVRALAANQDIILMD
                     EPFGALDPITRDSLQDLVKDLQERLGKTIVFVTHDMDEAIKLASRIAIMSEGRVIQFD
                     TPENILRHPANDFVEELIGEDRLLQAKPNATRVGEVMLNTAITITPEKSLQEAIKLMR
                     EKRVDTLLVTDNSNVLKGFIDVETLNKKRGKVSSVGDILNKHVFYVKESAYLRDTLQR
                     ILKRGLKYVPVVDEQNKVVGILTRASLVDIVYDVIWGEDPATTDVAESATPEESKEV"
     gene            14701..15342
                     /locus_tag="U2K71_001270"
     CDS             14701..15342
                     /locus_tag="U2K71_001270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315364.1"
                     /translation="MQQFFHQHGSEILSKGFEHLYISGLALIIGIIIAVPLGVLLTRF
                     PKAATIVIGLTSALQTIPSLALLALMIPLFGVGKLPAIIALFIYSLLPILRNTYIGMK
                     NVDWNYRDVAKGMGMTEFQSIFSVELPIAMPTIMAGIRLAAVYVIAWATLASYIGAGG
                     LGDLIFSGLNNYQPDLILAGTIPVTILALLADWLLGLLEHRLTPIALREENEE"
     gene            15339..16256
                     /locus_tag="U2K71_001271"
     CDS             15339..16256
                     /locus_tag="U2K71_001271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="osmoprotectant ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM7315365.1"
                     /translation="MKRILKMTMVLFSLLILASCSFPGLASNTDEDTISITGGITSEA
                     QILASLVAGMVEHYTDKNTAIINNLATTTINHQAMMNGDASISAARYTGTDLTTTLNL
                     PPEKDPKKAFATVKDEFEKRYGQTWFPSYGFENTYVFLVRKDTAQKYHLSKVSDLKNV
                     ADELVAGVDTSWINRKGDGYDGFQETYGFSFNSILPMQIGLVYDAVEAGKMDIVLGYS
                     TDGRIASYDLVMLEDDRQFFPPYDAAPVVDNKILADTPHLKEALNKLGHTISTEKMQE
                     LNYEADNNLVEPSVVAQRFLQENHYFEGK"
     gene            16253..16924
                     /locus_tag="U2K71_001272"
     CDS             16253..16924
                     /locus_tag="U2K71_001272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315366.1"
                     /translation="MKQLDKMNVFQQFFYYFQENGSYIFAQFIRHFLISIYGVLFAAV
                     VGIPVGIMISRRRKLANWVIRLANIIQTIPSLAMISILIIGLGLGVNVVIVTVFLYSL
                     LPIIKNTYTGMIQVDKNILDVGKGMGMTARQRLFMVELPLSVSVIMAGIRNALVVAIG
                     ITAIGAFVGAGGLGDIIIRGTNATEGTSIILAGALPTALMAIITDWLLGILERRLDPA
                     SRTSR"
     gene            complement(17465..17953)
                     /locus_tag="U2K71_001273"
     CDS             complement(17465..17953)
                     /locus_tag="U2K71_001273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannosyl-glycoprotein
                     endo-beta-N-acetylglucosamidase"
                     /protein_id="HEM7315367.1"
                     /translation="MTRKISKIGASLLVLAVATTIDYRQSEAKTIGNVVYRETAKSML
                     KKAMDNQPESSYWFPEDSLAWSFGKDTDTKYNTSVVPLAERVSKATLPNMNVTQTEEV
                     KVVALSAMNSSINGNASRGIDIFYANVFSYWQYIDQLVYGGALLVKDHRIAKSSCDGC
                     SP"
     gene            complement(18154..18420)
                     /locus_tag="U2K71_001274"
     CDS             complement(18154..18420)
                     /locus_tag="U2K71_001274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Txe/YoeB family addiction module toxin"
                     /protein_id="HEM7315368.1"
                     /translation="MSNYTVAIKNSAKVDLRKIKQTNLKKQFEEVIQTLKEDPYMPTQ
                     SFEKLRPTHEGRYSRRLNRQHRVVYKVDEENKVVEIYSAWTHYE"
     gene            complement(18420..18683)
                     /locus_tag="U2K71_001275"
     CDS             complement(18420..18683)
                     /locus_tag="U2K71_001275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEM7315369.1"
                     /translation="MANTTMNPSTARKNFYQLLKEVNENHTEIEIISDRSGNNAVLIG
                     LEDWRAIQETLFLEQTGTLDKVRDREKDDSSFTNIDDIDWEAL"
     gene            19159..19344
                     /locus_tag="U2K71_001276"
     CDS             19159..19344
                     /locus_tag="U2K71_001276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM7315370.1"
                     /translation="MALSYGNKKWSLDEAEIPTYEDTWHLRVEVKEEAENIGGCTLFG
                     KGFGNYDQDIKIKMFYS"
     gene            complement(19486..21309)
                     /locus_tag="U2K71_001277"
     CDS             complement(19486..21309)
                     /locus_tag="U2K71_001277"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HEM7315371.1"
                     /translation="MDYLKRLLVGKPLKSAENDEHKLTRFAALALLSSDALSSIAYGT
                     EQIVVVLVALSAAAIWYSLPIAAFVIILLISLTLSYRQIIHAYPHGGGAYVVSSENLG
                     KNAGLISGGSLLIDYMLTVAVSVSAGAEAITSAVPALYGHQVAISVTIVLLLMMLNLR
                     GLRESASFLLFPVYTFILVISLLIVVGLYNIVTGAVPLQATALPGAAVPGVSIALILR
                     AFSSGSSSLTGVEAISNAVPFFKKPRAKNAAATLTMMALILGFFFVGITFINYWYGIV
                     PEKEVTVLSQIGKAVFGHGILYYVLQFATALILAVAANTGFSAFPVLAYNLAKDKFMP
                     HMYQDRGDRLGYSNGIITLALGSIVLLFIFHGSTERLIPLYSIGVFIPFALSQTGMVV
                     KWKKEGKRWLSKSIANITGAFISYAIIAILFVYRLGDIWPFFIIMPIVMFIFYKIHDH
                     YKKVAEQLRLENDAKLHDYDGNTVLVLVGNVTRVNIGALNYARSIGDYVVAMHVSLDE
                     DVEKEKEIEAEFKKHFPDVRFSIVHSSYRSIENPIIRYVDIVSKNAAKQNYTTTVLIP
                     QFVPNRRWQNILHNQTSLRLRLRLSWRENIVVSTYSYHLKK"
     gene            complement(21907..24612)
                     /locus_tag="U2K71_001278"
     CDS             complement(21907..24612)
                     /locus_tag="U2K71_001278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YhgE/Pip domain-containing protein"
                     /protein_id="HEM7315372.1"
                     /translation="MQSIKNTLRLYLLDWKRIFKNPIATLLIIAIMIIPSLYAWFNIK
                     ALWDPYGNTGELPIAVYSADKPAEFQGKEVAIGEQVIESLHKNKQLGWQFVDSKEQLE
                     DGVRSGKYYAGIYLPKDFSEDLLSFTSGDIKKPKIEYTVNEKINAIAPKITDKGASSI
                     QSQITNEFIKTASSTLLKVFNEIGYDIDTNLVSINKVKDMILSTDENLDTIDGYTKQV
                     LELQSQLPEIKEKLNKANEFVDYIPKVDEMGEKVVALNDKMPELKDQAKIILDLQEKI
                     PEIQNAGKQLAEIDGDFASIQETMNQGIDEAKQGLTIIQQVQDILPDVKNLENQASDL
                     ANQTKSGASQLKDAIPSITNSVDVTLKSIGQVATTTGSLIDTVRTAIEDGKLTDDEKK
                     NISNVLNGFIDGISQQQNAIDKLVEFLTKLQTSAGNTKLQPIIDQLNQLKPLLSNLQN
                     RLSALNTAVQNEDTKAIDELLDQIDAVVNNINGIIAGINTGQISSTVTSLLDQVIDTI
                     GTAQGALSKADQIDFDSLLSSTKATVTNAVAILEKYQKEMPAIGQEIHDANTMLNGNM
                     DTIVNGINEGADLYNNELPIIQQKLGLAADFIQNDWPALKNEITGTLTTVNEKMPEVE
                     TAVNAAADLINNDWPNIKAGIQKAADAIRKGEQEVDLGEVIKLLKLDATKESDFFTQP
                     VELQTNALYPIANNGSASTPFYTALCLWVGAVLLSSVATTEYYLGKKDRNNFTKREQF
                     VARMLTFLTMAIAQSLIVTLGNIFLLGVDVQNPVYSVSFAVLIALAFMMIVYVLAALF
                     DNVGKGIAIIILVLSISGGGGNYPIQVSGKFFQMINPFLPFTHAVNLLRESAGGIYWP
                     NATKAIWIMIGLFVVFGIVGTAIYPFIEEKMTKFKEAAHESHIFH"
     gene            complement(24918..25865)
                     /locus_tag="U2K71_001279"
     CDS             complement(24918..25865)
                     /locus_tag="U2K71_001279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HEM7315373.1"
                     /translation="MRRFAINGYGEAQDVFEEIDAHPREVGSGHVRVEIKAFSVNPYD
                     VALRLGEMKEIRTLKFPYVPGNDGAGIVTEIGSDVTTVHVGDRVAVHAVGGTYGEEVV
                     LPSAKVAKIPDKMSWEEAAGMVTPGITAYNLINHLTEIQPTDTVMILGASGAVGSSLI
                     QLLHEKGIRILTSASSKNEEKVKKLGASAFAAYDKTNPGLQFADQADLVIDATKGSIK
                     GETGIQIMKPGGRYVALNDLPDLDLRQKKEGFYESFVPRKEYLDAEAFAGIIKAYQKG
                     AFHVFISMNLSASLKHVIQAHQLVEGHPPAGKIILSFEK"
     gene            26055..26324
                     /gene="rpsN"
                     /locus_tag="U2K71_001280"
     CDS             26055..26324
                     /gene="rpsN"
                     /locus_tag="U2K71_001280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S14"
                     /protein_id="HEM7315374.1"
                     /translation="MAKKSKIAKAKKQQETIERYAALRQELKAQKDYEGLRKLPLDAR
                     PERLKNRDLIDGRPRGYMRKFGMSRIKFRELAHKGLIPGVKKASW"
     gene            complement(26500..26862)
                     /locus_tag="U2K71_001281"
     CDS             complement(26500..26862)
                     /locus_tag="U2K71_001281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEM7315375.1"
                     /translation="MVKRGDIYFADLSPVVGSEQGGTRPVLVIQNNLGNHFSPTIIVA
                     AITAKMAKPKLPTHIGIKATGTGIERDSVILLEQIRTIDKSRLKEKVCHLDRPIMAEV
                     DRALKISVGIEALTPVTK"
     gene            complement(26873..27994)
                     /locus_tag="U2K71_001282"
     CDS             complement(26873..27994)
                     /locus_tag="U2K71_001282"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HEM7315376.1"
                     /translation="MVVAWHRPTKAVIHKKAITENVANEVARLPQGKELFAVVKANGY
                     GHGAIETAEAAVAGGASGFCVSNLDEGVELREAGFTQPILILNMVPYDALTVAVAHDL
                     SVTAGTREWLQAAAAVLEKSKLETPLSIHLKADTGMGRIGFCTPEEVKEAAAFIKESR
                     VLEWEGLFTHFSTADQADDTYWNLQKERFIEVLKKLPELPRYVHVSNSATALWHDETI
                     GNMIRYGVAMYGLNPSGHALPEVYPLQPALELVSELIQVKKLPAGEGIGYGETYITPE
                     AEWIGTIPIGYADGWPRKMQGFSLLVEGNYCETIGRVCMDQLMIRLPQEFPVGTKVTL
                     IGKNADKEITMQDIADQLGTIHYEVACGLGQRIPREYQE"
     gene            complement(28010..28360)
                     /locus_tag="U2K71_001283"
     CDS             complement(28010..28360)
                     /locus_tag="U2K71_001283"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HEM7315377.1"
                     /translation="MIKGIGIDAVELPRITRLIEEKPKFIARILTSDEMKLFQSLPFH
                     RQVEFLGGRYACKEAFSKAWGTGIGKVTFQDVEILKNENGQPVVTRSPHEGNVWVSIT
                     HTNETAFAQIILED"
     gene            complement(28490..30001)
                     /locus_tag="U2K71_001284"
     CDS             complement(28490..30001)
                     /locus_tag="U2K71_001284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM7315378.1"
                     /translation="MKFKELELSPELLKSVERAGFEEATPIQAETIPLALAGKDVIGQ
                     AQTGTGKTAAFGLPMLEKIDPDRHELQGLVIAPTRELAIQTQEELYRLGRDKKVRVQA
                     VYGGADIGRQIRGLKDRPHIVVGTPGRMLDHINRHTLKLGTVQTLVLDEADEMLNMGF
                     LEDIEKIISQVPDQRQTLLFSATMPPAIKNIGVKFMKNPHHVKIKAKEMTADLIDQYY
                     VRAKEYEKFDIMTRLFDVQTPELTIVFGRTKRRVDELARGLEARGYRAEGIHGDLSQQ
                     KRMSVLRSFKSGHLDILVATDVAARGLDISGVTHVYNYDIPQDPESYVHRIGRTGRAG
                     KGGMSVTFVTPNEMDYLHVIENLTKKRMTTLRPPTEKEAFKGQLGAAVEQIETKLAEN
                     GLDKYLQTADKLLEEYSAQDLVALLLKTTAKDPADAVPVKITPERPLPMQKKGYNKNG
                     KRGGGNNRNRRDGGNYRGNKSKGGYSKNNNSQKDGGKRHNDKKRGFVIRSNND"
     gene            complement(30323..31675)
                     /locus_tag="U2K71_001285"
     CDS             complement(30323..31675)
                     /locus_tag="U2K71_001285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HEM7315379.1"
                     /translation="MNLTIQEIAQAVGSECSALTKITNVEFDSRKITEGSLFVPLAGT
                     RDGHEFIQQAITNGASASFWSLPVEQAPKNFPVIPVADPLKAMQQLATYYLEKVGAEV
                     VAITGSNGKTTTKDLTASVLSEKYKTYKTQGNYNNNIGLPYTILHMPQDTEKIVLEMG
                     MDHANEITELSLMAQPKAAAITMIGEAHIENLGSREGIAKAKMEITDGLVSDGLLVVP
                     ADEPLLTPLTATLVQTVTTFGIGNGDVHAEVTAKGKAQTDFVVEGETFTIPLPGSYNV
                     TNALIAYTIGRFFGLSIPEIRQGLAHVSITQNRTEWLTAGNGAAILSDVYNANPTAMG
                     LVLDTFKNLPTKGRRIAVLADMLELGKESLNMHAQMSEHIDPEKFAMIFLYGEQMHAL
                     KEELNQKYPQLPVFYLEKEKNKLIDAIKQNLEATDSIMLKGSNSMGLFEVVEQLQQMK
                     "
     gene            complement(31828..32904)
                     /locus_tag="U2K71_001286"
     CDS             complement(31828..32904)
                     /locus_tag="U2K71_001286"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HEM7315380.1"
                     /translation="MKITLLYGGRSAEHEVSILSAFSVLNAIYYNYYQVQLVFITKEG
                     QWVKGPLLTEKPASKDVLHLSWDPSGQTEEGFTGKVINPGEIKEEGAIVFPVLHGPNG
                     EDGTIQGFLETLNMPYVGAGVLTSACAMDKIMTKYILQAAGVPQVPYVPVLKNQWKEN
                     PKKVFDQCEGSLLYPMFVKPANMGSSVGITKAENREELQNALATAYQYDSRAIVEQGI
                     EAREIEVAVLGNEDVRTTLPGEVVKDVAFYDYEAKYINNKIEMQIPAEVPEEVYQKAQ
                     EYAKLAYTMLGGSGLSRCDFFLTNKNELFLNELNSMPGFTEFSMYPLLWENMGLKYGD
                     LIEELIQLGMNRYHQRQSFFEKNE"
     gene            33100..34845
                     /locus_tag="U2K71_001287"
     CDS             33100..34845
                     /locus_tag="U2K71_001287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/ABC1/UbiB kinase family protein"
                     /protein_id="HEM7315381.1"
                     /translation="MAQTNPRSRLAEIIRVFINYNVVPNFVQQKNPEQVKKAFEELGP
                     TFIKIGQMLSVREDLLSSAFTQTFKTLQDSVPSDTFSTVKKTIETELSLSLSDIFDDF
                     SKSPFASASMGQAHRAKLKNGDAVVVKIQHPNIAEEIRLDLQLFERAIPLIKYIPETS
                     VVDLKGVLQEVKRSLINEMDFLKESQNGEQFYQKNNGWKEIRSPKIYDAFCSKKVIVM
                     EEMSGKNLNHLMNAENKTETFITGIQNKQLKQEVAKLLVENFMKQVFDDGFFHADPHP
                     GNLLFHVLTKEEQTQASRKTETVHEKEFGSFAFRASTSAEDPVAPYTINYIDFGMMGH
                     LSAGLRQKLTQAVLALYTKDAYRIEKAVLRLCQQEGSFDESRFHQELTSFLEQYYDSP
                     IDEINLQEVFTHVVTICHQNNLQFDRDITLLLKAFGTLEGVIRVLDPEVSLMEVASPF
                     AQHYFLTHLDVEDTLKQSGLDLLEGMKAAPKIPQQLHHLLEMWTSGQGKVNLELKKQD
                     KLLSRIESMINRLVFGMILAALIVGSSLLVQAAPVENAEVVSLLGIFTYAIAAFVIIF
                     LAIDALIQMYKKRKK"
     gene            complement(35125..35562)
                     /locus_tag="U2K71_001288"
     CDS             complement(35125..35562)
                     /locus_tag="U2K71_001288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YaiI/YqxD family protein"
                     /protein_id="HEM7315382.1"
                     /translation="MRLFIDGDGSPVKEDVIKLGESYGLDVVIVTSVDHYTTKEYANH
                     VQIVYVDRGADSADYKIVGMVKKNDFLITQDYGLASLVLPKGVRVFHHSGKEFDEANI
                     GTLLEQRYHSGKLRKAGIRTKGPKPFTEKDHMSFRQALKKAID"
     gene            complement(35546..36919)
                     /locus_tag="U2K71_001289"
     CDS             complement(35546..36919)
                     /locus_tag="U2K71_001289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315383.1"
                     /translation="MFEKLKRFGRNFTQTTPFILSNTVIFIPYLLFLGLTDSHNWLTI
                     LPFTLFYAFRMTGLFLVNSLRFGLDSYTLLMIALLIGGAGSLLGILGVFYFPLFYFSS
                     VLLGLSAAWLVPANTTVNFHEKTQGFINMDGKKYLFAAIWLFLLYQAIELPAPTQIAA
                     SFTIYTLFYVMAYHTVSHYPRYELDFKDIKRNLVATRELVLFLIFFGLLFLLRNARLL
                     FDERLFDLAIYGFLLLFILFVFVLGHQKKEWKLPSWLNLFTFLNGMLGNFLFLFSSFY
                     VGILYGLEKLSIFMYLPYAAGLIAAKIVGPWILKRLTWSPLAMQLLGLSSSLLVLLIP
                     NGFAIGVFLLSFWHVVTGSWLNKEYYQTDTAIPMDRRIVVKYTTQNKGSVIHQFLLMT
                     LLFVLAKEMGEPIHLLLITLNHESVPIESVQLLILAKWINVGLLLSGLVTVYHLWKKA
                     KKNEAIH"
     gene            complement(37241..37729)
                     /locus_tag="U2K71_001290"
     CDS             complement(37241..37729)
                     /locus_tag="U2K71_001290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7315384.1"
                     /translation="MEKIKVTENELDELMAVIQEVWPEAFVPIIGQKQVDYMLKTYQS
                     KKQIQKELTEGVSYFLLKSEEKTVGYTAYEEINGKIYLSKLYLLNKVRGQGLTSSVFR
                     WYEELAAQTKEREIFLRVNQGNHQAIEVYKHEGFEITEELISDIGQGFQMVDYKMKKA
                     LA"
     gene            complement(37751..38353)
                     /locus_tag="U2K71_001291"
     CDS             complement(37751..38353)
                     /locus_tag="U2K71_001291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEM7315385.1"
                     /translation="MSKKGSVLQKCLQIMPIIGLFFFFVLILYAYHHGIFRSSTSLQA
                     FIQQFGKYAVMIFVLLQVIQVIIPILPGGISSVAGMLMFGNGFGLLYSCIGLVIGEAI
                     GFLLVRYYGVAFVQLILSPKKYQKFDELLTRKTKDIKKVLVVTLLLPFAPDDLICLVA
                     GLTKLSFREFMQIVIFLKPWSVGVYSMIMLFLFHQAQGHL"
BASE COUNT        10920 a         8263 c         7031 g        12182 t
ORIGIN      
        1 agcgcctacg agataacctc ccataaacaa agcagagatc gaagcaccac cagcaacaag
       61 tgaatacatg ataaaagcag tacttggagg aatgaccata cctgtcggag ctgacgcgat
      121 attgaccgct gtcgcaaact tccggtcata tccttcttcc acttgctgag ggatcaacac
      181 tccaccaatc gcggtagaag cggcaatggc cgagctagag atggaaccaa ataaagcatt
      241 ccctaacaca ttcgtatgag ccaaagcccc cggcacgcgc ccgacgaaca acatcgcaaa
      301 atccactagt ttcttagcta tccctccatt attcatgata atgcccgaga gaacgaaaaa
      361 cggaatagcg atcagcgaaa aactattgat acttcccacc attttttgag ctgaactaaa
      421 agcagaaaca tcaaacggta taactaacaa taaagtcatc atcgatgaga atgcaatact
      481 tatagcaatc ggcatcccta atacaagcaa cacaacaaat acaaaaaata acactaatcc
      541 ggcttctatt gccattttat tcctccattt ttatattttt tcttgaattg ctggcttgtt
      601 gcttaatgta tcgattagat tgatcaggct atagataatg atgagtaccc ccgatacagg
      661 tactgacaga taaacatatc ccataggaat acttaaagaa ggcgagatct gccccattgt
      721 caatgtgaca gcattccatc cgccatacac catgatcatc gcagcaaata ccagaaataa
      781 actttgaaca acaacttcta aaattttact ttttttgcca gttaattttt cacttaaaac
      841 cgttacggct aaacgtgctt tttgtcccac aacatacgcg gaagacaaca tggcaaacca
      901 gacaagaccg aatcgaacaa gctcttccgt gaccgtattt ggatttccta aaaccgttct
      961 ggaaaatact tgccataaaa caacagcgac catcccaaca agcaaaacag ctcccaggat
     1021 ctctaatacc ttgtttaaaa tcattttcat tacagctacg ctccttgctc ttggatcttt
     1081 tgataaaggt ctttgtaggt aggatcgttt ttgaatatat catgaagtgg ggcaactgcc
     1141 tctcgaaacg gtgtattatc tacatcattg aattggactc catgttcttc ttgtgctaaa
     1201 gctttttctt cttcaattgc ttttgcaaaa gcttcaattt caaaaaccgt tgattctttc
     1261 gctgcttctt caatggcttt ttcctgttca tcagttagct gttctttgat ggcatcattc
     1321 atgatgatga tatctggcac acgtgtatgc tcatcatagg aatagtattt agctaccccg
     1381 ccatgtccag ctgtatacaa gacaaattca ttgttttctg atccgttgat caaattggat
     1441 tgaagagacg tgtacacttc atcacttccc ataggtacgg gcgaaccgcc taaaagctct
     1501 accattttga tagcggtttc gctttgcatc acacggatct tctttccctt caaatcttcc
     1561 ggcgtatgga tcggaccgtc taccatataa aagctgcgct gaccagaatc ataataggtc
     1621 aatccggtaa atcctaaatt tttggtcgat tggtagattg gctccattat ttcttcatta
     1681 tccatcacct taaagaaatg ttcttcatca ttgaatagat aaggaacgga aaaaatcgaa
     1741 taatctttag agaaactttc caacgctgag gcacttactt ttgtgaaatc tactgctcct
     1801 gactgtgtca attcgatcag ttcacgttct ccacccagtt gtccatctgg gaaatactgg
     1861 atctgtacgg aaccatctgt tttttctttt actaaacgcc caaattcatt cattgcatca
     1921 attactggct gttgcttgca taagcaaagc gcaaggtaac tatctcattt acgtctgcat
     1981 ctgcgacagt tgccacacta gaattatttc cgcagccgct tagacctagc agacctaggc
     2041 taagcgaact tgctgcaaac agcagtgatg tttttttcat atgggtttcc tttcttttaa
     2101 caataagaga cgagcaattc tgtcaaacaa agcaccgtta acgactgtcc ataaggcatt
     2161 gctgtgattc cgatattccg ataaaaatct aaactgtccc ccattcctgt cccgattgaa
     2221 acgttttgaa cttctccttt ttcatcgatt tgttcaagca atccttggat agcacgataa
     2281 gcgacttctt tatattcttg tggtaaataa cgtttatgga ccgctttcaa aataccgtaa
     2341 gcaaatcctg cagttgcaga agattctacg taagaagaag gatcatccaa caacgtatgc
     2401 cataaaccgc tttcagactg ataagttttt aatgcacgca cttgtgcttc aagtgtttcg
     2461 atcaataatt tgcgcaatgc atcttctttc gataattcta agatttcaat gatctctgga
     2521 atagcaatcg tcaaccagca gtttccacga gcccaaaatg cttcggcata gttatgattt
     2581 ccttcaaacg tccagccgtg ataccacaat cctgttcgtt tatccatcaa gtatttgata
     2641 tggatcataa actggtaacg agcttcttct aaatattctg ggcgattcaa cagtttcccg
     2701 atttttgcca aaggaagcac tgtcatcatc aatgtatcgt cccacaactg attcttattt
     2761 tccggtccat atgtggcatg ctgtaacccg tcttcatttg tccgaggcat gtcattcatg
     2821 acccattctg cccattgttc taaataaggc aaatagcgtg aatctttcgt gtcttcatac
     2881 aagtaagcca tagtcaatag cggtgccatt gtattgacgt ttttgggcgg agcgccttct
     2941 tccatacgat cctcaaacca ttcattgacc acttgataag cgcgaggatt ttttgtcatc
     3001 tggtaatttt tataaatacc atatagaccg acaccttgtg gccaattcca cactgtccag
     3061 cttttgtcat ctactttcag tccatcaaag tccaacaaaa attcaccatt atcgtctttg
     3121 atcgttgtca ggttttcaac gagcaggtca attttttctt tcactaattc tttaggtaca
     3181 aaaatctctt ctttaataat catctttacg ccaccttttt aaacgctttc attttatatc
     3241 cttaatatag cagtaatacc gtccactata aatatcgctt tttgaacaaa aactgtctaa
     3301 aattgaccta aatgttaaat aacaaaagta actttcagtt acttggaaat taaaaaaaga
     3361 acttggagaa acaacgtttt ccaagttctt tttcatctat tttcttatct tttttctacg
     3421 ctttcttttg cttatccgtt gtaagcatca atgatgatct gtttcaattc agagatcaat
     3481 ggttctttcg gattagctgt cgtacattgg tcttcgtaag ctaattcagc catacggtct
     3541 acggttgtat ctagtgtttc ttgtgtgact ccttgagctt ttagactcat gttgatgccg
     3601 acagattttc ctaattcata aacagccgtt gctaaagctt cgactaattc ctctgttgtt
     3661 tcgcctttca gacctaagaa ttttgcgata tctgcgtaat ctgtatctgc acggaaatag
     3721 tcatatttag ggaacatcgc atgtttttgt ggatcttttg cattatagcg gataatatgc
     3781 ggtaaaagaa tcgcatttgt ccgtccatga gggatcccgt attccccgcc gattttatgt
     3841 gcgactgagt gacaaatacc taggaatgcg ttagcaaacg ccataccagc cattgtagaa
     3901 gcgttgtgca ttttttcacg tgattccata tcaggtgttt ttactgattt ttctaaatag
     3961 tcaaagacta acttgattgc ttgtaggctc aatccacgtg tataatctga agccatgact
     4021 gaaacatagg attcgattgc atgtgttaac acatccatcc cggtatctgc tgtgacagat
     4081 gctggaactg acatgacgaa ttgaggatcg acgattgcga catctggcgt caatgcataa
     4141 tctgctaacg gatatttcac atgtgtttca ctgtcagtga tgaccgcaaa tggcgttact
     4201 tcagaaccag tccctgatgt tgtcgggata catacgaatt gtgttttttc tggtttgtcg
     4261 atcttatatg tccgtttacg gatatccaag aatttttgtt ttgcaccaaa gaaagatgtg
     4321 tctgggtgtt catagaacat ccacattccc ttagctgcgt ccattgcaga accgcctcca
     4381 agagcgatta ctgtatctgg cttgaagtct gccatcagtt tcgttcctgc gtatacggta
     4441 tttgtcgatg ggttcggttc aacatctgag aagacttcga tcttcacatc atttttacgt
     4501 cgtgccaata cttcacgaac tgtatcgcag taaccgaatt ggaccatacc agggtcacaa
     4561 acgatcatga cacgttcaac gttttccatt ttctctaagt acaatagaga gttcttttca
     4621 aagaagattt ttggcggtaa tttgaaccat tgcatgttat ttctccgttt cgctacagtt
     4681 ttgacattga ttaagtttac agcagagacg ttacgagaaa cagagttctt accataagaa
     4741 ccgcatccca aagtcaatga cggaatcatt tcgttataga tatcaccgat gccgccttct
     4801 gcagatggtg tattcactaa aatacgacaa gctttcattc gaagaccgaa tttgacatgc
     4861 aactcttcgt tttctgaatg gataactgct gtatgtccta atcctccaag attcaacata
     4921 ccttcacaca aatcaaacgc atggtccgtg ttgttagctt tgatcatggc aagaactgga
     4981 gaaagttttt ctcttgatag cggataatct gggcctacgc cttccaattc agcaatcagc
     5041 attttcgttc cttttggtac actgattcct gccaattttg cgatttccat cgcagaatgt
     5101 ccaacgattg atggattgac cgcatatttt ccttcgttca tcactgcatc ttctaatttt
     5161 tgcaattcat ctggttttac gatataaact tggtgtgctt ggaattctgc ttttactgct
     5221 gcatagattt ctttatccac gatcacagct tgttcagaag cacaaatcat tccattatca
     5281 aatgttttcg aaacgatcaa atcgttcact gcacgtttaa tttttgctgt tttttcaata
     5341 taagctggta cgttacctgg acctacacct aaagctggtt ttcctgtaga gtaagcagac
     5401 ttgaccattc cagctccacc tgtcgctaag acgatcgcga tacctggatg gttcattagc
     5461 atagaagtcg cttcgatcga aggatgttcg atccattgga tacaattttc aggagctccg
     5521 gcagcgatag cagcatcacg taccactcgt gctgcttctg cagaagattt ttgggcactt
     5581 ggatggaaag caaaaacgat tggatttctt gttttcaacg cgatcattga tttgaaaatc
     5641 gttgtggaag tcggattcgt tgttggagtt actccgcaaa ccacaccgac tggttcagca
     5701 atttcaatca accctttttg gacatcttca ttgatcacac caactgtttt atcatgtttg
     5761 atattatgcc aaatatattc ggacgcatac atatttttga ttgctttatc ttcgtaaaca
     5821 ccgcggcctg tttcctctac agccatcttc gctaatggca tatgttgatc caaagctgcc
     5881 attgccattt catgcacgat atggtctact ttttcttgat caaagctttc catttctttc
     5941 aatgctgcat ttgctttttc tgccaaatca tcaatcattg cttgtacgtc aattgtcttc
     6001 gttttttctt tttctattac tttagccatc attatcctcc taatgggtca tggttatttg
     6061 ttaaataatt cacatgtata tcatacaata tttatttttc tatgtaaaca cttttttgtg
     6121 atttttttca cagttatttt taaatataaa attgctttat ttacaggtgt ttgtatagaa
     6181 aaatatataa agttatttat tattatttat atgacacacc cgattattca atattgagca
     6241 atatatccca tgctcttatc tctttctttt ttgaataaaa aatgaggtag gacatctttc
     6301 tgccctacct cgtctttaaa taagctttga tacttatttt tcttccttct cttctgtctc
     6361 ttccagcgtt tctgttgttt cagtttttac ttcaactgtc tcagctggtt cttctgttgt
     6421 ttcaacagta gtcgttgcat catttgttac gacagtccct ggtttgaccg ttttgattgc
     6481 tgcacgatcg tactcaagga agatcccttc gcagtctaaa acaactgtcc gtttgtcatt
     6541 gtcgatttct gaaacaactc catgtaatcc gccgatcgtt acgactgaat cacctggttt
     6601 catgctatct aacaagtttt gacgttcttg ctgttgtttc ttttgcgaac gtgacataaa
     6661 aaaccacatt gctaatagcg cgataaacat cacaagcatt ggtaatgttc ccattttttt
     6721 cacctcacta attctgtatc tacaaaaaac actttagcag aaaaatgctg ataacactag
     6781 ttttttactt aatttttaaa agattttcct ttagaagctt tttgcgtttt ctttattgaa
     6841 cccgtattct tcaaaaaagg cttctctaaa ttcaagcaaa ttatcatcca tgattgcttg
     6901 acgtacttgt ttcattagat tcaacaagaa gtataaattg tggtaagaag tcaaacgaat
     6961 cccaaatgtt tcatcacatt tgatcaaatg gcggatatag gcacgtgtat agtttttaca
     7021 tgtataacaa tcacatttct catcaattgg acggaaatca tgggcatatt tcgcattttt
     7081 cactactaga cggccttgag aagtcataca tgtgccattt cgagcgattc gagtaggcaa
     7141 gacacagtca aacatgtcta ctccacggat aacaccatcg atcaatgaat cagcagcacc
     7201 gacacccatc aagtaacgtg gtttgttttc ggggatcaac ggtgtagtga attctaatac
     7261 acgattcatt tctgatttag gttccccgac agataacccg ccgattgagt aaccagggaa
     7321 atccatcccg actagatctt tcgcgctttg acgacgaaga tcttcaaacc ctgctccttg
     7381 gatgattcca aataatcctt gacggtctgg attagcatga gctttcaatc ctcgttctgc
     7441 ccatcgagac gtacgttcta ctgatttttt cacgtagtcg taactttcat cgaatggtgg
     7501 acattcgtca aaactcatca tgatatcaga gcccaattta ttttggatgt tgattgcttt
     7561 ttctggtgat aaaaacatac gcgaaccgtt caagtgattt ttgaaatgca cgccttcttc
     7621 ttcgatattc cgcatatcac ttaatgaaaa aacttgaaag ccgcctgaat ctgttaaaat
     7681 cggttgatcc cagttcataa atttatgcag tccgccagct tcttctacta agtcttcacc
     7741 agggcgtagc cataaatgat atgtattact taaaataacg ccagcaccca tttctttcaa
     7801 ctcttctggg gacattgttt ttactgtagc taatgttcct actggcatga acattggtgt
     7861 ggggaaagtt ccgtgtggcg tgatcaattc ccctaaacgc gcgccagtat gtttttcttt
     7921 cttgattaaa cgatagcgaa tggcaggttg cgtcattcat ttccctactt tctatataaa
     7981 atatcttgac aactcccacc atcataatcg tttcaagtgc tttttgcaag ttccctcggc
     8041 ttccttctac tttatttaat attctttttt ttatcctttt tttatgaata acaaaaaaaa
     8101 gaaaaacatt atgacagaaa tattttttga tacatggtat attggtgtta ttaaagaaaa
     8161 ggagggaaaa atcatgcaat cttctgcaga attaaaacgc caagcaaaag agagtctaaa
     8221 aggacgttgg ggacaagctg ttttactaaa tctaatccct acattgattg gtattgctac
     8281 tattcttctt attatgattc ctattatctt gtttgccacc accatatatg atggatcagc
     8341 gagcgtctca gaagcgacaa cttcaggagg cggtagttca ggaggcggtg gcggaattat
     8401 ttccagcatc attagcgctt tgttcatgag cggtatttct tggacttatc ttgatatttt
     8461 acgtggacaa aaagatcaga tcgaaccatt caaagatgca tttcgtggat tcaaaggttt
     8521 gtttatcggc ggagtgatca gcttagctgt gctgatctcg atctttacaa cattatgggg
     8581 tttgctatta gttattcctg gaatcattaa aagctattct tattctcaaa gctattttat
     8641 ttactacgat atcgtccaag aaacaggaca aacacctaaa gtactggata cgatcactgc
     8701 aagtcgaaaa ttgatggatg gttataaagg acaacttttc tggttagatc tttcatttat
     8761 cggctggcat atcttagcat tagcaactgc tggtattggt tatctatggc tcagcccata
     8821 catttctgca acaaaagcag cattttatga acaattgcca aaagatattt aaaagagagt
     8881 gtgacttgtg accgaggggg tggcaggaga acaccgttta tcgagaggca gtaaaagaaa
     8941 cattcagccc attagcaaaa aggaggtgtg acaacattca tgtcacacct cctttttgta
     9001 tagttccagt aaaaagaaaa tcaacttaga aacagttaat cattcctctt cttctgcttc
     9061 cggtaatcga tggaacttga tcagttcatt caccgcgatt ggtacgatag ataaaatccc
     9121 taccaagaac cagtgattca aactgatgtg tactaggccg aatagttctt gcgtaaatgg
     9181 cagcaaagca acagccgttg taatagctaa tgccagtaac gccatttcta ccaaagcttt
     9241 attggtcgct aaattgactt tgaagatcga ttggctgctg cgtacattga acacatgcag
     9301 aatcgatgaa taagctaaga tcaagaatgc aacagtttga ccgacatgct gacttgcgtc
     9361 tacccatggg ctgatagtat cgacataact acctaaataa taaccaaaca aggttacgat
     9421 ggtgaagacc cctgcattta tggcaatttt ttgcaataaa cctcgagcaa agatcccttc
     9481 atttttcgga atgggtggct gatccatgat gccgtgttcc gctggctctc ttcctaaagc
     9541 aaatcctgga agtccatcag caactacatt gacaaacagt agttgcacag ctgtaaatgg
     9601 tgctccccat cctaacagca tcgcaatcaa aacaataaag atctccgaaa tattacagct
     9661 tagcaaaaag ttgatagctt tacgaatatt ctgataaacc gaacgtcctt gactgacagc
     9721 gtctacgatc gtcgcaaagt tgtcatccgt caaaatcata tctgcggcac tttgcgccac
     9781 atctgttcct gtgatcccca tggcacaccc aacgttactt gctttcaacg ctggtgcatc
     9841 attcacacca tccccagtca tcgctacgac tgcacccgta cgttgccaag atttgacgat
     9901 ccggatcttg tcttctggcg ttacccgagc atagacagag agagctttga cacgcgcatc
     9961 taattcttcg tctgataatt gatgcagttc tgatcctgaa agtgcttctg acggatcatt
    10021 caagatacct aattctttcg ctatggcact ggcagtcacc acatgatctc cagtgatcat
    10081 gactgttttg atgccagcct ttttcgctcg cttgattgct cctttacttt ctggacgtgg
    10141 tggatcgatc attccgatca gaccaatcaa acgtaagtct ttttccagtg cttcagaaga
    10201 aatatcttgc ggttcttcgt cataagtagc ataaccaaca gcaatcactc tcagtgcccg
    10261 tttaccaaag cgatcattaa cgatcgccgc ttgttctaca tcgcctgaac taaagcgcgg
    10321 agcaagaaca tcgaatgctc ctttggtgat tgagatgtat ttttcgccca tttggtgaac
    10381 tgttgtcatc atttttcgtt cagaatcaaa aggaagctcg tttactcgtg gatatttttc
    10441 ttctaattct gcctttgtat gataattctc ttcaacagcc cttacgatgg ctacttctgt
    10501 tggattacct ttgataacaa ggtcgtcatc ttcttgctcg accgtcacat ctgtacatag
    10561 tgcagccatt ttcaatattt ccatcgcgct gttcgtcatt gaatcttcga tattcgttac
    10621 ttcatcttct ttgctccata cccggcggac gcgcatttca ttttgtgtca gcgttcctgt
    10681 tttgtctgag cagataacat tagcggttcc tagcgtttca actgctggca actgtcggat
    10741 gatcgcatgt ttcctcgcca tcttctgcac accgtatgca agtgtcaacg taacaataac
    10801 agtcaaggtt tccggtacag ctgcaacagc caaggaaatc gaagtcatga acatctcaag
    10861 gagtggttct ccttgcagtt caccaatcac aaatactaaa gccgcagccg ctaaagcgat
    10921 catactgatt cgtttgccta gctgattcaa tcggacctgt aaaggtgttt tttgcatagt
    10981 attttcgttc aaaagatccg cgatcttacc catttctgta gccatgccaa tggcagtgac
    11041 gatcgctttc ccacgacctg cagcaaccgt acatccttta aataccatat taagttggtc
    11101 acctaatgac tcatcttctt cagcaataaa tgctgcatct ttttcaactg cttcactttc
    11161 tccagtcaac gccgattctt ctacacgtaa ttgtgatgct tcgatcaaac gggcatcggc
    11221 agaaatagca tcaccggact ctaaaacaag aatatcaccc actaccagtt gctctgactc
    11281 aacagtggtc actttgccgt ctcgtatgac agttgccgtt tgtttattca tatcttgcaa
    11341 ggattccagt gctttttctg cgtttccttc ttggacaatc gccaataccg agttgatcac
    11401 aacgatagcg atgatcagca gtccttcaaa taaatcccca tgttctgtag caaatgcggt
    11461 ataaaaggaa atcaccgctg ctacaagaag gatgatcgtt gtaaaatcgg acaagcttcg
    11521 aaagaatttt ttgatcatcg attctttcgg cgcctcatcg aatttattca atccctgctt
    11581 ttttcgccgt tcatccactt cttgtgaaga caacccagta tctggatctg cttctgttac
    11641 cttctttact tcttctattg tctgcttgta ttcttccatc ctattcctcc tttaaaatct
    11701 tcgtacaaaa aaaggtactt attttactat aacatccttt ttttatttac ttcaaataaa
    11761 gggcttatcg attatttgtt aagaaagcgt aaaatttaaa gtgcttatat ttgcaaacgc
    11821 taccaaagga tgctattata tagatgtaag gaaggaggat accatcaaat agacagaaga
    11881 atttctcgat agaacagtgt aagcaaacaa agttgttaga agtacaggtt tcacccagac
    11941 atgcgtgtag agtacaaacc tataaatagt gaatgcgtat ggatgtttgg atcacgaagt
    12001 atccgaaaca agaagtaatg cgatacgtaa caattgaata acgtaaacgt ctgaaaattg
    12061 aataggaaca agagatgttt caaatgttga agatgtaacg aaaacaaaat ttttgtttaa
    12121 catgaagaaa ttcaacaccg tgctttgcat gtatgatgga aagttacaat catttctgtt
    12181 tatttgaatc cttccgaggt acaagaaagc cgagcttatc gagagctcgg ctttttcttt
    12241 gtcataaaat ctttgtaaag cttttttgtt attcaaaact agcggctttt cataacctta
    12301 tttcacgaac atcgcatctc caaaactaaa gaaacgatag cgctcttcaa tcgcatgctg
    12361 gtaagcagat aaagtcaatt ctcttcctgc aaaggcacta actaacatga ctaaagtcga
    12421 ttttggcaaa tggaaattag ttgagaaagc ttctaccact ttgaattcat agccaggcgt
    12481 aatgaaaata tcagtccatc cgctgtctgc tttgatttct ccattaaatt tcgttccaat
    12541 cgtttctaat gttcgaattg aagttgttcc gacagccacg attttcccgc catttttacg
    12601 tacttggttc aatcgctcag ccgcttcttc cgtcaaacgg tagaattcac tatgcatatg
    12661 gtgttcttca attttatcta cgctgacagg tcggaatgta cctaacccta catgtagtgt
    12721 cagatagact aattcaacac cctttgcttc gatctcatca agtaattctt tcgtaaaatg
    12781 caatccagct gttggcgctg cagcagaacc attttctttc gcatagacag tttgatagcg
    12841 atctgggtct tccagacgtt ctttgatata tggaggcaat ggcatttctc ccaatgattc
    12901 aagaatctct agaaaaattc cttcatactc aaagtcaatg atccgtccgc cgtgttccaa
    12961 ttcttctttc actactgctt gcaatctgcc atcaccaaaa gagattcttg tcccaacttt
    13021 ggcacgtttc gcaggtttga tcaatgtctc ccacgtatct ccttgtgtat tcgttagaag
    13081 aaggacttct aaatgcgcac cagtatctgg tttttctcca tataaacgag caggcagaac
    13141 tcgtgtatta ttcataacta gcgcatcacc aggctgtaac tcttcaatga tttcatggaa
    13201 atgtttatct tccatctctc cagttttccg atccaaaaca agtaaacgag aatgatcgcg
    13261 gtcttttaat ggtgtttgtg cgattaattc ttccggcaag tcaaaatcaa aatcttctgt
    13321 tgttaacata tcgtcactca ctttctatca tgtgcttcat cattctatca tgtttttcgt
    13381 tctgttggca aaatcagcac tttttcactt ctattttttg ttttcctaaa taaaaaaact
    13441 accctatttg tattttttca ttgttttagg catactagta atagatagtt cttcattgaa
    13501 aatagaaaaa gaggtggcac catgattgaa tttcaaaatg tgtcaaaaat ttataaaggt
    13561 ggaaaagtcg ctgtagagga cgtcaatctt tcatttgaaa agggtgagtt catttgtttt
    13621 atcggtacga gcggaagcgg aaaaacgacg acgatgcgga tgatcaaccg aatgactgat
    13681 ccaacacaag ggaaaatttt gattgatgga aaatccatcc aagaaattga tccagtggaa
    13741 ttgcgacgtc agatcggcta tgttatccaa agtattggat tgatgcctca catgacgatc
    13801 cgtgaaaata tcactcttgt ccaaaaattg ttgaaagtca gtcaagaaga acggaataaa
    13861 acagctgaaa aaatgattga tcttgtggaa ttgccccgtg agatgttaga tcgctaccca
    13921 catgaattat ctggtggtca gcagcagcgt attggagtcg ttcgtgccct tgcagcgaat
    13981 caagatatca tcttgatgga cgagccattc ggtgcgcttg atccaatcac tcgtgattcc
    14041 ttgcaagacc ttgtcaaaga tttacaagag cgtttgggaa aaacgatcgt atttgtaacc
    14101 catgacatgg atgaagcgat taaactagca agccgtatcg ccatcatgag tgaaggccgt
    14161 gtgatccaat ttgatacacc agaaaatatt ttacgtcatc ctgcaaacga tttcgtagaa
    14221 gagttgattg gagaagatcg cctactacaa gccaagccaa atgcgacaag agtcggcgaa
    14281 gttatgctaa atacagcaat cacgattaca ccggaaaaat cattacaaga agcaatcaaa
    14341 ctgatgaggg aaaaacgtgt agatacgttg ttagttaccg ataattcgaa tgtattaaaa
    14401 ggatttatcg atgtagaaac gttgaataaa aaacgaggaa aagtatccag tgtcggcgac
    14461 attttgaata aacatgtttt ttacgtaaaa gaatccgcct atcttcgtga taccttgcaa
    14521 cgcattttaa aacgcgggct gaaatacgtg ccggtcgttg atgaacagaa caaagtcgta
    14581 ggtatcctga ctcgtgcatc attagtggat atcgtctacg atgtgatttg gggagaagat
    14641 cccgcaacta ctgatgttgc agagtcagct actcctgaag aatctaagga ggtctgacct
    14701 atgcagcagt tttttcatca gcacggttca gaaattctct caaaaggttt tgaacatctt
    14761 tatatttcag gacttgcttt gatcattggt atcatcattg ctgtaccgct tggtgtctta
    14821 ctgacacgtt ttccaaaagc tgccacgatt gtgattggct taaccagtgc cttgcagaca
    14881 atcccttctc ttgctttatt ggcattgatg atcccgctat ttggcgtagg taaattacct
    14941 gcgattatcg cactctttat ttattcatta ttgccgattt tacgtaatac gtacattggg
    15001 atgaaaaatg tcgattggaa ttatcgggat gtggcaaaag gcatggggat gactgagttc
    15061 caatccattt tttccgttga attgcccatt gctatgccaa cgatcatggc tggtattcgt
    15121 ttagctgccg tttacgtgat tgcatgggct acacttgcct cttatatcgg cgctggtggt
    15181 ttaggcgatt tgatttttag tggactgaat aattaccaac cagatctcat tttggcaggt
    15241 acgatccctg taacaatttt agcactattg gctgattggc ttctaggtct cttggaacac
    15301 cggctgacac cgatcgcact tagagaggag aatgaagaat gaaacgtata ctaaaaatga
    15361 ccatggttct tttctctctt cttatacttg cgagttgttc cttccctggt cttgccagca
    15421 atacagatga agacaccatt tcgatcacag gtggaatcac ttcagaagca cagatcttag
    15481 caagtctagt tgccggaatg gttgaacatt atacagataa aaataccgca atcatcaaca
    15541 atctggctac tacaactatc aaccatcaag cgatgatgaa cggagatgct tcaatttcag
    15601 cggctcgcta cacaggaaca gatttaacga ctacattgaa ccttcctcct gaaaaagatc
    15661 cgaaaaaagc gtttgctacc gtgaaagatg aatttgagaa aagatacggt caaacgtggt
    15721 tcccgtccta tggttttgaa aatacgtatg tctttcttgt tcgaaaagac accgcacaaa
    15781 aataccatct atctaaagtc agcgatctga aaaatgtagc ggatgaatta gtagctggtg
    15841 tagatacttc ttggatcaat cgtaaaggag acggttatga cggcttccaa gaaacttatg
    15901 gcttctcctt caattcgata ttacctatgc agattggact tgtttatgac gcagtcgaag
    15961 caggaaaaat ggatattgtt cttggctact ctacggatgg acggatcgcc agttatgatc
    16021 tagtcatgtt ggaagatgat cgtcaattct tcccaccata tgatgctgct ccagttgtag
    16081 acaacaagat attagctgat acgcctcatt taaaagaagc cttaaataag ttaggtcata
    16141 ccatcagtac ggaaaaaatg caggaattga actacgaagc agataataat ctagtggaac
    16201 catcagtagt agcacaacgt ttcttacaag aaaaccacta ttttgaaggg aagtgaagca
    16261 attggataaa atgaatgtct ttcaacaatt tttctattat ttccaggaaa atggctccta
    16321 tatctttgca caatttatcc gtcacttcct gatttctatc tatggtgtcc tctttgcagc
    16381 agttgtcggg attccggttg ggatcatgat ttctaggaga cgtaaattag caaactgggt
    16441 gattcgtttg gcgaatatca tccagacgat cccttctcta gccatgatct ccattttaat
    16501 cattggatta ggcctgggag tcaatgtcgt gattgttact gtatttttgt attccctctt
    16561 accgatcatc aagaatacgt ataccgggat gatccaagta gacaaaaaca tccttgatgt
    16621 cggcaaaggc atggggatga ctgctagaca acgactattc atggtagagt taccactctc
    16681 tgtttctgtt atcatggcag gcataagaaa cgcgttagtt gttgccatcg ggataaccgc
    16741 gatcggtgca ttcgtcggtg ctggtggtct tggagatatc attatccgag gaaccaatgc
    16801 aacagagggc acatcgatta ttttagcagg tgcattgcca actgcattaa tggcgatcat
    16861 cacggactgg cttttaggta ttttggaaag acggctagat ccagccagcc gtacatcacg
    16921 ctgatgttat tgtaaaaaac aaaagcttct gcgaccattt gttggtcgca aaggcttttt
    16981 tagttctgtc atttttaagg caataatcca ctctgattct tccctcctta tgggcaaaga
    17041 gttgcttttc ttagctattt atccattccg tatactccaa cttcatactg ccaacgggtt
    17101 catatctgca tccaccgact aagttttttt cttatccgct aaggcatttt gccaatccat
    17161 tttcccatca ctcgtcatgg aattcatacc acatgatctc taaatactcg tctgcttgtt
    17221 ctttgaattg ggcatcaatt cttacatcct ttggcgtggc tttcatttaa gccttttcca
    17281 tcagtcctcg attgttcgat gccttcagct gcatcatcaa aactggtgac tgccgtatcg
    17341 gtaacttcga tcatctttta tacaatcggg aacttgccat cctcatcctt ggctaaaaac
    17401 atatctagcc attccagctt ctcgccatgg gaggtttgcg gttcctaaaa ccggtacacg
    17461 attcttatgg actgcatccg tcgcaactag acttggcgat acgatgatcc ttcaccagaa
    17521 gagcaccccc gtaaaccaat tgatcgatat attgccaata agaaaagaca ttggcgtaaa
    17581 agatatcgat ccctcgtgat gcattgccgt tgatactgct attcatggcc gaaagcgcca
    17641 cgactttaac ttcttccgtt tgagtgacat tcatgttcgg caatgtcgct ttggataccc
    17701 gctctgctaa aggaacgaca ctggtattgt atttcgtatc tgtatctttt ccaaaagacc
    17761 atgccaacga atcctctggg aaccaataag acgactccgg ttgattgtcc attgcttttt
    17821 tcaacatact tttggctgtt tctcgataaa cgacatttcc aattgtcttc gcctcacttt
    17881 ggcgataatc gatggtcgtt gccactgcca acactaacaa cgatgctcca attttactga
    17941 ttttccttgt cacacttctt cctccttgtt ttacataaac ccgtcaacat tccattccta
    18001 aactcaaaca atgacccttc aaaggtggag atataggaac aaattaaaga aatcatgcaa
    18061 taaatccaag aaggttggtt ttagaacaaa aaaagagtct cctttttggg gagactcttt
    18121 gttaactatc taaaaaggtt ggctcttctc acttcactca taatgtgtcc aggcagaata
    18181 aatttctaca actttatttt cctcatcaac tttgtaaacc actcgatgct gtctattcag
    18241 cctgcgtgag tatctgcctt catgtgtagg tctcagcttt tcaaaagact gcgtgggcat
    18301 atatggatct tccttcaatg tctgaatcac ttcttcaaat tgtttcttta aattcgtttg
    18361 cttgattttt cttaaatcca ctttcgcaga gtttttgatt gcgactgtat agttactcat
    18421 caaagagctt cccaatcaat atcatcgata ttcgtaaagc tactgtcgtc tttttcgcgg
    18481 tctcgtactt tatctaaagt acctgtttgc tcaagaaaaa gggtttcttg aatcgctctc
    18541 cagtcttcca gcccaatcaa tacagcattg tttccactac gatcactaat aatctcgatt
    18601 tcagtatgat tttcatttac ctctttcaat aactgataaa aatttttacg agcagtgcta
    18661 ggattcatcg ttgtattagc cattgtctct cctccttttt ctgatataat tatagcgtac
    18721 ttgattaagt acgtcaaaaa aatacaataa agcacttaaa ttcagcaaac ttttcctctt
    18781 ttttacatag agaaaaagac ccctttttag ggaggatctt tgtccacgct cacttcttac
    18841 tcctttacga ctagcggtag ccaaacgtaa agtaattggt aacaacgtat catctttagc
    18901 aaaggtcgaa tccaaagcct gtgagtctat gcaacaacac caatacacga aggttcctcg
    18961 ttgcagtgtg acttgattga tatcttgaat aacctgagga tacgcacgaa agatttgtaa
    19021 cggttattca aaactgattt tggccgtcaa gtcttctact gcctcaaaaa tgattgcctc
    19081 tttcgtctcc tgtcattcat gttcactatc gatttgatac ttgcttagac cgttgggttt
    19141 gtgaatcacc aattaaaaat ggcactgagt tatggtaaca aaaaatggtc acttgacgag
    19201 gctgagattc ctacttatga agacacatgg cacttaagag tcgaggtaaa ggaagaagct
    19261 gaaaatatag gaggctgtac attatttggc aaaggattcg gaaattatga tcaagacatc
    19321 aaaatcaaaa tgttttacag ctaaatagga agaataataa catttcatac gctgttccca
    19381 tctttaatgg cttatagagg gcacctttag ctgagaataa gacaaaacag accctctagc
    19441 ttcaattaag agctagaggg tctgtttttg attttaaaac ttggtttatt tcttcaaatg
    19501 gtagctatag gtgctgacaa cgatattttc acgccaagac aatcgcaatc ttaaccgcaa
    19561 acttgtttgg ttatggagaa tattctgcca gcgtctgttt ggtacaaatt gaggaatcaa
    19621 cacagtagtt gtatagtttt gtttcgctgc atttttactt acgatatcta cataacggat
    19681 aattggattt tcaatcgagc gataagaaga gtggacgatt gaaaaacgga catctgggaa
    19741 atgcttttta aattctgctt cgatttcttt ttctttttcg acatcttcgt ctagcgaaac
    19801 atgcatcgca acgacataat caccgatcga acgtgcgtaa ttcaacgcac cgatatttac
    19861 ccgtgtcaca ttcccgacta atacaagaac ggtattcccg tcataatcat gtagttttgc
    19921 atcattttct aaacgaagct gttctgctac ttttttataa tgatcatgga tcttataaaa
    19981 aatgaacata acgattggca taatgatgaa gaaaggccaa atgtccccta accgatagac
    20041 aaacaaaatc gcaatgattg catatgagat gaacgcaccc gtaatattgg caatcgattt
    20101 gcttaaccat ctttttcctt cttttttcca tttgacgacc atccctgttt gagataaagc
    20161 aaatggaatg aacacgccga tagagtataa cggaatcaag cgttctgttg agccatggaa
    20221 aataaacagc aatacgatcg aacctagcgc tagtgtgata atcccattgg aatagcctag
    20281 tcgatcccca cgatcttgat acatgtgagg catgaattta tcttttgcca gattgtatgc
    20341 aagaactgga aaagcagaaa aaccggtgtt agcagcaact gctaaaatca gcgctgtagc
    20401 aaattgaagg acgtaataca aaataccatg tccaaagaca gctttaccaa tttgagacaa
    20461 aacagtgact tctttttcag gaacgatccc ataccaatag ttgataaatg tgatccccac
    20521 gaagaagaaa cctaagatca atgccatcat cgtcaatgta gcagcggcat tttttgctct
    20581 tggcttctta aagaatggta cagcattact aatcgcctcc accccagtta aagaggaaga
    20641 accagaagaa aatgctcgta aaatcaaagc gatagaaaca cccggcacag ccgctcctgg
    20701 aagtgcagta gcttgcaatg gcacagcacc tgtcacgata ttatataaac caacaacgat
    20761 cagtaacgag atcacaagga taaatgtgta caccggaaat aataggaacg atgcagattc
    20821 cctcagccca cgaagattca gcatcatcag tagcaaaacg attgtcacag aaatagcgac
    20881 ttgatgaccg tagagtgctg gcaccgctga tgtgatcgct tcagcacctg ctgagacaga
    20941 tactgctact gtcaacatgt agtcgatcaa taaagagcca ccggaaatca agccagcatt
    21001 ctttccaaga ttctcactgc tgacgacata tgctcccccg ccatgaggat aagcatggat
    21061 gatttgacga tatgatagcg ttaatgaaat caataaaata ataacaaaag cggcaatggg
    21121 tagcgaatac cagatggctg cagcagacaa agcgactaaa acgacaacga tttgttccgt
    21181 accatatgca atagatgata acgcatcaga cgacaataat gccaaagcag caaatcgggt
    21241 taatttgtgc tcatcatttt cagcagattt taaaggctta cctactaaca acctttttaa
    21301 gtaatccaat ttctaaactc ctttacacac aattttactt caagttttat agtcgaaaat
    21361 ttatagtaaa caagatgtta tagtacgctc actgacctta aaagtcaata aactttaaga
    21421 aaataattgg aaaaaaacag aaaaatatca agcgcttttt caaacgcttt aatacagcaa
    21481 aaatctaaca tttttttatg ggctttttct ttgaattcta taataaaagg atagaacttg
    21541 acttagaaaa tcaagttcta tcctttttga tatcttaact tttggaagag agatgtaagg
    21601 ctgctttcta cccatcattc ttactactcg aagcatagcg cagttgtcac aacctcttga
    21661 taaacggtgt ttaaaagctt gcatctgcgg taataagccc aaccaaatta aaaacataag
    21721 aagctgtttt tatttggtcg ttcttattac ttgatgcaga gcagcttttt tacaacctct
    21781 tggtacacgg tgttcaatca gctgaccctc ggtattagct caaaaaaacg gcaaaatatg
    21841 aggaactatt tttccatttt tttctctaat actcaggtca aaacactgat ttcacaacct
    21901 ctttttttaa tgaaaaatat ggctctcatg cgccgcttct ttgaatttcg tcattttttc
    21961 ttcgatgaac ggatagattg ccgttcctac gattccaaag acaacaaaca aaccgatcat
    22021 gatccagatt gctttcgtag catttggcca ataaattcct cctgcagatt cccgtaacaa
    22081 gtttaccgca tgagtaaatg gcaagaacgg attgatcatt tggaagaatt tccctgagac
    22141 ttggatcgga tagttcccgc ctccgccaga gatcgacagt actaagatga taatagcgat
    22201 ccctttaccg acattatcga ataatgcagc caagacatag acgatcatca tgaacgctaa
    22261 ggcgatcaag acagcaaatg acacgctgta gaccggattt tggacatcta ctcctagtaa
    22321 gaaaatattc cctaatgtaa cgatcaagct ttgcgcaatc gccattgtca aaaatgtcag
    22381 catacgagca acgaattgtt ctcgtttcgt aaaattgttt cggtctttct ttcctaaata
    22441 gtattctgtc gtagcaacac ttgaaagaag aacagcgccg acccacagac atagtgcagt
    22501 ataaaacggg gtgctagctg aaccgttatt tgcgattgga taaagcgcat tggtttgtag
    22561 ctcaacaggt tgtgtaaaga agtcactttc ttttgttgcg tctagtttca ataatttgat
    22621 gacttcgcct agatcgactt cttgttcgcc ttttcggatc gcgtctgctg ctttttggat
    22681 accagctttg atatttggcc agtcattgtt gatcaagtcc gcagcagcgt tcacagctgt
    22741 ttcgacttct ggcatttttt catttactgt tgtcaatgta ccggtgattt cattttttag
    22801 tgctggccaa tcattttgga taaaatcagc ggccaatcct aatttttgct gaatgatcgg
    22861 taattcattg ttgtataggt ctgccccttc attgatgcca ttgacgatgg tatccatatt
    22921 tccattcagc attgtattgg catcatggat ttcttgtcca attgcaggca tttctttttg
    22981 atatttttct aaaattgcca ctgcattcgt tacagttgct ttcgttgagc tgagaagcga
    23041 atcaaagtcg atctggtccg ctttgctcag tgcgccttgt gcagtcccaa tcgtatcgat
    23101 gacttggtct agtagagaag tgacagtgga actgatttga cctgtattga taccagcaat
    23161 gatgccattg atattattaa caacggcatc aatttgatct agcaattcat caatagcctt
    23221 cgtgtcttca ttttgaacag ctgtgttcaa agcagatagg cggttttgca gattgcttag
    23281 caagggtttt aattgattta gctgatcaat aattggctgt aatttcgtat ttccagcact
    23341 tgtttgcaat ttagtcagga actcgaccag tttatcgatt gcattttgtt gttgagatat
    23401 tccgtcaata aagccgttca atacattgct gatgtttttc ttttcatcat cagtcagctt
    23461 accatcttca atagctgttc ttacagtatc tattaacgaa cctgtcgttg ttgccacttg
    23521 tccaattgac ttcaatgtaa catctacact gtttgtgatg cttggtatcg catctttcag
    23581 ctgagaggca cctgattttg tttggtttgc tagatcactt gcctgatttt ctaaattttt
    23641 cacatctggc aagatatctt gtacttgttg gatgatcgtc aagccttgtt ttgcttcatc
    23701 gattccttgg ttcattgtct cttggatcga agcaaaatcg ccgtcgatct ctgctagttg
    23761 cttaccggca ttttggatct ctgggatttt ttcttgcaaa tcaaggatga tttttgcttg
    23821 atctttcaac tcaggcattt tgtcattcaa tgcaacgact ttttcaccca tctcatctac
    23881 tttagggatg taatccacga attcatttgc tttattcagt ttttccttga tttcaggtag
    23941 ttgactttgt aattcgagga cttgttttgt gtagccgtcg atcgtgtcca aattttcatc
    24001 tgttgataaa atcatatctt taactttatt gatacttact aggttcgtat cgatgtcgta
    24061 acctatctca ttgaatactt tcaataaagt cgaactggct gttttgataa attcatttgt
    24121 gatttgcgat tggatcgaac ttgctccttt atcggtgatt ttcggtgcaa tcgcattgat
    24181 tttttcattt accgtatatt cgatttttgg tttcttgata tcaccactag taaaactcag
    24241 cagatcttct gagaaatcct ttggcaagta aatacctgcg taatactttc cggaacggac
    24301 tccgtcttct aattgttctt ttgaatcaac gaattgccat cctaattgtt tatttttatg
    24361 aagcgattcg atgacttgct ccccaatggc cacttcttta ccttgaaact ctgctggctt
    24421 gtctgcactg taaacagcaa ttggcagctc tccagtattt ccatatggat cccataatgc
    24481 tttgatgtta aaccacgcat atagcgaagg gatgatcatg atcgcgatga tcagaagtgt
    24541 tgcaatcggg tttttgaaaa tccgtttcca gtcaagcaaa tataatcgaa gcgtattttt
    24601 gattgactgc atagttctcc cccttcttct tttttcagct gaattacttt accatgtatt
    24661 tcatactttt ttcaatcggc ttttttcaaa aaagtcgaat ttttggacat atcacggcaa
    24721 ttgtctcatt gtaatctctt acatattgag aaataattaa ggattacagc ctttacttta
    24781 tcatgaaata aaagggaaag ataattttat gccaaagttt ttatctatta tacgtatacg
    24841 caaaaaagct aaaacatttt ttgtctttgc ccctacaaaa atgtttcagc ttttttcgtt
    24901 ttttattcct gtatttctta tttttcgaat gacaggatga ttttcccagc aggaggatgt
    24961 ccttcaacta gctgatgtgc ttggatgaca tgcttaagtg aagcagacag attcattgaa
    25021 ataaaaacat gaaaggctcc tttttggtag gctttgatta tgccagcaaa ggcttctgca
    25081 tctagatatt cctttcttgg aacaaacgat tcataaaagc cttccttttt ttgccgcaaa
    25141 tctagatctg ggaggtcatt taatgcaaca tacctgccgc ctggtttcat gatctggatg
    25201 ccggtttctc ctttgatact tcctttcgtc gcatcgatta caaggtctgc ctgatcagca
    25261 aattgcagac ctggatttgt tttatcataa gcagcaaatg ctgaagctcc taattttttt
    25321 accttttcct catttttact ggatgcactg gttaagattc ggattccctt ttcatggaga
    25381 agctggatca gacttgaccc gaccgcacct gatgctccta gtatcattac agtatctgtc
    25441 ggctggattt ccgtcaaatg gttgattaga ttataagccg taattcctgg cgtgaccatt
    25501 ccggctgctt cttcccaact cattttatct ggaatttttg ctacttttgc tgatggcaac
    25561 acgacttctt ctccgtaagt cccgccgact gcatggacag ctactcgatc acctacatga
    25621 acagttgtga catctgaacc aatctctgtc acaataccag cgccatcatt acctggtaca
    25681 taagggaatt tcaatgttcg tatctctttc atttctccta aacgcaaggc aacatcataa
    25741 ggattcacac tgaatgcttt tatttctaca cgaacatgtc cagaaccgac ttctctggga
    25801 tgagcatcga tttcttcaaa aacatcttgt gcttctccgt aaccgttaat tgcaaatctt
    25861 cgcatggtca ccatctccct ttttctttaa gtttacgttt atgcctttat tttgacaaaa
    25921 aaaatgtctt ttcgttttgg cgcatgattc aaaacaaaat agttgtcttt tttcgcgtaa
    25981 cgtgttaaat tatttatcta aaagcgtaaa gattacgatt tacgctaata aaaaataatt
    26041 ggaggaattt gttgatggct aaaaaatcaa agattgccaa agcaaaaaaa cagcaagaaa
    26101 cgattgaacg ctatgctgct ttacgccagg aattaaaagc gcaaaaagat tacgaaggct
    26161 tgcgtaaact tcctttagat gctcgtccag aacgattgaa aaatcgtgat ttaatcgatg
    26221 gacgccctcg cggatacatg cgtaaattcg ggatgtcccg tataaaattc cgtgaattag
    26281 cacataaagg tttgattcca ggagtaaaaa aagccagctg gtaatccaag tctggctgta
    26341 atgtaaagga ggagggcgca tggaccactc cttggatcat caaaaaagat acgttcgctt
    26401 cttctagcag aaatgctggt actagaagca gacgtatctt tttttacgca cagctggtcc
    26461 ttttttatat gtaaaggctg catggttact tcgctatatt tatttagtaa caggagtcag
    26521 tgcttcgata cccacactaa ttttcaatgc tcggtcaact tctgccataa tgggtcgatc
    26581 caaatggcag accttttctt tcaatcttga tttatcgatc gttctgatct gctccaacag
    26641 gataacggag tctctttcta ttcctgttcc agtagctttg atcccaatgt gagtcggaag
    26701 cttaggttta gccatttttg ccgttatggc cgcaacaatg atcgtcgggc taaaatggtt
    26761 acctagatta ttttgaataa ccaaaactgg gcgtgttccg ccttgctccg atcccacgac
    26821 tggagaaaga tcagcaaaat aaatatctcc tctttttacc ataggatcct ccttattctt
    26881 gatattctct tggtatccgc tgtcctaatc cgcaagccac ttcatagtgg atcgtaccta
    26941 attgatctgc tatatcctgc atagtgattt ctttgtctgc atttttgccg ataagcgtca
    27001 cttttgttcc gacaggaaat tcttgaggca atcggatcat cagttgatcc atacatacac
    27061 gcccaatcgt ttcacagtag ttcccttcta ccaatagaga aaaaccctgc atctttcgtg
    27121 gccagccatc tgcataaccg atcggtatcg ttccgatcca ctctgcttca ggagtgatat
    27181 atgtttctcc atagccgatt ccttcacctg ctgggagttt tttgacttga atcaattcag
    27241 aaaccagctc aagtgctggc tgcaatggat aaacttccgg tagtgcgtgc ccagagggat
    27301 taagtccgta cattgctact ccataacgaa tcatattgcc gattgtttca tcatgccata
    27361 aagccgttgc actattgctg acatgtacat atcgaggaag ttcgggaagt tttttcagca
    27421 cttctataaa gcgttctttt tgcagattcc aatatgtatc atctgcctga tctgctgtag
    27481 aaaaatgggt aaataaacct tcccattcta atacacgact ttctttgatg aatgcagctg
    27541 cctcttttac ttcttcaggt gtacaaaatc cgatacgacc catcccagtg tctgctttta
    27601 gatgaatcga aagaggcgtc tctagcttac ttttctctaa aacagcagct gctgcttgca
    27661 accattcccg tgtacctgca gttactgata aatcatgagc aacagctaca gtcagtgcgt
    27721 cgtaaggaac catattcaag ataaggatgg gctgagtaaa tccagcttct ctcaactcga
    27781 ctccttcatc taaatttgaa acacaaaagc ccgaagctcc acctgcaact gctgcctcag
    27841 ctgtttcaat agctccatga ccgtatccgt tcgcttttac tactgcaaac aattcttttc
    27901 cttgcggaag tcttgcaact tcatttgcta cattttcagt tatagctttt ttatgtatca
    27961 ctgctttcgt tgggcggtgc caagcgacaa ccatcgtttt tccttctttc tagtcttcca
    28021 aaataatttg tgcaaaagct gtttcatttg tatgagtaat actgacccat acattgcctt
    28081 catgaggtga acgcgtcaca acaggctgac cattttcatt ttttaaaatc tcgacatctt
    28141 gaaaggtaac ttttccgatc ccagttcccc aagctttaga gaaagcctct ttgcaagcgt
    28201 aacgcccgcc taaaaattct acttggcgat gaaaaggcaa ggactggaat agtttcattt
    28261 catcagaggt caagattcgt gcaatgaatt ttggtttttc ttcgatcagc cgagtgatcc
    28321 taggaagttc gactgcatcg atcccaattc cttttatcat atgcttttct cttttcaata
    28381 aatttagaac actctcattc taccaaatta taaccatttt gccacaaata agtccacaaa
    28441 taacaaaaat gaaagtttct cttaaaaaag agaaactttc attttatgtt taatcgttat
    28501 ttgaacggat tacaaatccg cgttttttat cattgtgacg tttaccgcca tctttttggc
    28561 tgttattatt tttgctataa ccgccttttg atttgtttcc acgataatta ccgccgtcac
    28621 gacgattacg gttgtttccg ccaccacgtt tgccattttt attgtatcct tttttctgca
    28681 ttggcaatgg gcgttctggt gtgatcttca ctggcactgc atcagctgga tcttttgctg
    28741 tcgtttttag caaaagtgca actaaatctt gtgcagaata ttcttctaat aatttatctg
    28801 ctgtttgcag gtatttatct agaccatttt ctgcaagttt tgtttcgatt tgttcaactg
    28861 cagcacctaa ttgacctttg aatgcttctt tttcggttgg tggacgtaaa gtcgtcatcc
    28921 gttttttcgt caagttttca atcacatgca agtagtccat ttcatttggt gtaacgaaag
    28981 tcacagacat accgcctttt ccggcacggc ctgtacgtcc gatacggtga acgtagcttt
    29041 ctggatcttg agggatatca tagttgtaca catgagtgac acctgaaata tctaatccac
    29101 gtgcagcaac gtctgtcgct acaaggatat ctaaatgacc gctcttaaat gaacgcaata
    29161 cgctcattcg tttttgttga gacaaatcgc catggattcc ttctgcacgg taaccgcgtg
    29221 cttctaatcc gcgtgccaat tcgtctacac ggcgttttgt acgtccgaag acgattgtta
    29281 attcaggtgt ttgaacatca aacaagcgag tcatgatatc gaatttttcg tattcttttg
    29341 cgcggacata gtattgatcg atcaagtcag ctgtcatttc tttcgctttg atcttcacat
    29401 gatgtggatt tttcatgaat ttaacgccaa tattcttgat agctggcggc atcgttgctg
    29461 agaaaagtaa tgtttgtctt tgatcaggaa cttgtgaaat gattttttcg atatcttcca
    29521 agaagcccat attcaacatt tcatctgctt catccaaaac taatgtttga acggttccaa
    29581 gtttcaatgt gtggcggtta atgtggtcaa gcatacgtcc tggtgtaccc acaacgatat
    29641 gtggacgatc tttcaagcca cggatctgac ggccaatatc tgctccgccg taaacggctt
    29701 gtacacgtac ttttttgtcg cgccctaaac gatataattc ttcttgtgtt tggatagcta
    29761 attcacgtgt tggtgcgata actaaacctt gtaattcgtg acgatctgga tcgatttttt
    29821 ctaacattgg taatccaaat gcagctgttt tccctgtacc tgtctgtgct tgtccaataa
    29881 cgtcttttcc agctaatgct agtggaatcg tttctgcttg gataggcgtt gcttcttcaa
    29941 aaccagcgcg ttcgactgat ttcaacaatt ctggtgataa ttctagttct ttaaatttca
    30001 aatgaatcct cctatatggt tgttaactga ggatgtagct tttttgctac cccgtcgatt
    30061 tgttcggtca aagtgtattg tttctttttg acagtggcca cgcacgtttt tatccattct
    30121 tagcccctgt ggcaccaccg aaaaatcact ttgcgaagtt taggcattag aaaagccaaa
    30181 cgggtcgcgt tcagctttat cattattatg acatctggct tcaacaagtt gtttctatgg
    30241 caaaaccagt ccaatttctc ttcatctttc ggcaataggt tattctaaca tagtttttcg
    30301 ttttctgcaa gtttacagat ttttatttca tttgctgaag ttgttctacc acttcaaata
    30361 agcccatgct gttgctgcct tttagcataa tactatctgt tgcttcaaga ttttgtttga
    30421 tagcatcaat caatttattc ttttcttttt ccaaatagaa cacaggtagt tgggggtatt
    30481 tttggttcaa ttcttctttt agtgcatgca tttgttcccc ataaaggaaa atcatggcga
    30541 atttctcagg atcgatatgt tcactcattt gcgcatgcat atttaaagac tctttcccta
    30601 attccagcat atctgctaac acagcgattc tgcgtccttt tgttggcaga tttttaaaag
    30661 tatctaatac cagtcccatg gctgttggat ttgcattgta gacatcgctc aaaatagctg
    30721 caccatttcc tgccgttagc cattcggttc tgttttgagt gatagaaaca tgggcgagcc
    30781 cttggcgtat ctcaggtatg gacagtccaa agaagcgccc gatcgtatac gcaatcaaag
    30841 cattcgttac attataactt ccaggcagcg ggatcgtaaa tgtttctcct tctactacaa
    30901 aatcggtttg tgcttttcct tttgctgtca cttccgcatg aacatcgcca tttccaatac
    30961 cgaatgttgt gactgtttgt actaatgttg ccgttaaagg tgtcagcaac ggttcatctg
    31021 cgggcacaac gagtaatccg tctgatacaa gtccgtcagt gatttccatt ttggctttag
    31081 cgattccttc tcgcgaccca agattttcta tgtgtgcttc accgatcatt gtgattgctg
    31141 cagcttttgg ttgagccata agtgaaagtt ccgtgatttc attggcgtga tccatgccca
    31201 tttccaaaac gatcttttct gtatcttggg gcatgtgcaa aatcgtgtaa ggaagaccaa
    31261 tattattgtt gtaatttcct tgcgtcttat atgttttgta tttttcagac aagacagacg
    31321 cggttagatc tttcgtggtg gttttaccat tgcttcctgt aattgcaacg acttctgcgc
    31381 ctactttttc caagtaatag gttgccaatt gctgcatggc tttcaaagga tctgctacag
    31441 gaataactgg aaaatttttt ggagcttgtt caacaggcaa gctccaaaat gaagcagatg
    31501 ctccgtttgt gatcgcttgc tggataaatt catgtccatc tcgcgtacca gcaagaggaa
    31561 cgaataaact tccttcggtt atcttccggc tgtcaaattc gacattggta atctttgtca
    31621 gtgcagagca ttcgctgccc acggcttgtg caatttcttg tattgttaga ttcattgaga
    31681 tctctcctaa aaaaggttgt gaaaaaagcg tttggcctaa acggcgggtt cgcatgactc
    31741 tcacttccaa gtaaatttct ctattcgtat ttagaagcta gaatgaacgc ggttacgcaa
    31801 tcactccagc ctcttcttta tttctcttta ttcatttttt tcaaaaaaag attgacgctg
    31861 atggtatcga ttcattccta actggatcag ttcttcaatc aaatcaccgt atttcaagcc
    31921 catattttcc cataagagtg ggtacatact gaactccgta aatcctggca tagagtttaa
    31981 ttcattcagg aataattcat ttttatttgt caaaaagaaa tcgcaccggc tcaatccgct
    32041 tccacctaac atcgtgtaag ctaacttcgc gtactcttgc gctttttgat aaacttcttc
    32101 tggcacttcg gctggaatct gcatttcgat tttattattg atatattttg cttcataatc
    32161 atagaatgct acgtctttta cgacttcacc aggcaaagtc gtccgaacgt cttcatttcc
    32221 taatacagca acttcgattt cgcgcgcttc aattccttgt tcaacgattg ctcgagaatc
    32281 atactgatag gctgttgcta aagcattttg cagctcttct cggttttctg cttttgtaat
    32341 gccgacacta gaacccatat tcgccggttt gacaaacatc ggataaagca aagaaccttc
    32401 acattgatca aatacttttt taggattttc tttccattga ttcttaagta ctggtacata
    32461 aggaacttgc ggcacaccag cagcttgtaa aatatacttg gtcatgattt tatccatggc
    32521 acatgcactg gtcaatacgc ctgcgccgac ataaggcata ttcaatgtct ctaagaagcc
    32581 ttggatcgtt ccatcttccc cgtttggccc atgtaaaact ggaaaaacga tggctccttc
    32641 ttctttgatt tcgcccggat tgatcacttt tcctgtaaag ccttcctctg tctgtccact
    32701 tgggtcccat gaaagatgca agacatcttt gctggcaggt ttttctgtta gtaatggacc
    32761 tttgacccat tgtccttctt ttgtaataaa tacgagttga acttggtaat aattataata
    32821 aatggcattt aaaactgaaa atgcggaaag aatggacact tcatgctctg cgctgcgtcc
    32881 gccatatagt aaagtaatct tcaaaagatt gtcctcctgt attctaaatc gttcagtgat
    32941 ttactctcat attttagcac aaaacaaaaa taaacgaata acttatttat aaaaaaacga
    33001 gccagcttct aaaagaacga gcgtcattta ttttttgtca gctagattat ttgtcccgtt
    33061 ttttataaaa agttatgata aagaaaagga gggatgatga tggcgcaaac aaatccacgc
    33121 agccgcttgg cagaaatcat tcgagtgttc atcaattaca atgtagtgcc aaactttgta
    33181 cagcaaaaaa atcccgaaca ggtaaaaaaa gcctttgaag aactaggccc caccttcatc
    33241 aagataggac agatgctttc tgttcgagaa gatttgcttt cttctgcctt cactcaaaca
    33301 ttcaaaacat tacaagatag tgtacctagc gacacatttt caacggtcaa aaagacgatc
    33361 gaaacagaac tttctctttc attatcagat atattcgatg atttttcaaa atcgcctttt
    33421 gcctctgctt ctatggggca ggctcatcgt gccaagctga agaacggtga cgcggtagtc
    33481 gttaagatcc aacaccctaa catcgccgaa gagatccgac ttgatcttca actatttgaa
    33541 cgagcgatcc ctttgatcaa atacattcct gaaactagcg tagttgatct aaaaggggta
    33601 ctccaagaag taaaacgttc cttgatcaat gaaatggact tcttaaaaga aagtcaaaat
    33661 ggcgaacaat tctatcaaaa gaacaatggc tggaaagaga tccgcagccc caagatttat
    33721 gacgcttttt gttcgaaaaa agtgatcgtg atggaagaaa tgtctggtaa aaacttgaat
    33781 catttaatga acgccgaaaa caaaactgaa acttttatca ctggaattca aaacaagcag
    33841 ctgaaacaag aagttgcaaa gcttttagtt gagaacttta tgaaacaggt gtttgatgat
    33901 ggttttttcc atgctgatcc tcatccaggg aatctgctat tccatgtact aacaaaagaa
    33961 gaacagacac aagctagccg aaaaactgaa acagtccatg aaaaagagtt cggttccttt
    34021 gcttttcgag cctccacttc tgcagaagac cctgttgcgc catataccat caactacatc
    34081 gattttggta tgatggggca tctttctgca ggacttaggc aaaaactcac acaagccgtt
    34141 ttagcgctgt atacgaaaga tgcttatcga atcgaaaaag cggtccttcg actttgtcag
    34201 caagaaggct cgtttgatga aagtcggttc caccaagaac tgacaagttt tcttgaacag
    34261 tattatgatt ctcctattga tgaaatcaac ttgcaagaag tattcacaca tgtcgtgacg
    34321 atttgtcatc aaaataatct gcagttcgat cgtgatatca cgcttttgct gaaagcattt
    34381 gggacattag aaggggtgat ccgcgtcctc gatcctgaag tttccctcat ggaagtcgct
    34441 tcgccttttg ctcaacatta ttttctcact catctggatg tagaagatac attgaaacaa
    34501 agcggattag acttattgga agggatgaag gctgcgccta agatccctca acagctccac
    34561 catctattag aaatgtggac ttcgggacaa ggaaaagtca atttggaatt gaaaaagcaa
    34621 gacaaattat tgagcaggat cgaaagtatg atcaatcggc ttgttttcgg tatgatttta
    34681 gctgcattga tcgtagggtc ctccctgctt gtccaagcag cacctgtaga aaacgcagag
    34741 gtcgtttcct tattagggat atttacgtat gctattgccg cattcgtcat tatttttttg
    34801 gcaatcgatg cattgatcca aatgtataag aagaggaaaa agtagaaaaa ggttgtgaca
    34861 gaaagaatca gcttcaagaa ataagaaggg gttttacata aattgtttct caaatttttg
    34921 tgaaaattcg gcttattttt cgaagaggct acttctgaaa caccgagaat aaaaaatgtg
    34981 aaaaagctgt tctgcaccca gtaataagaa caatcaaaaa gggaacatgg aacacattta
    35041 tcctgtttcc ctgttccctt tttgatgtat tcgagcataa ataaaatacc ttcgttacct
    35101 actatcttct gtacttggta aaccttagtc aattgccttt tttaacgctt gacgaaaaga
    35161 catatgatct ttctccgtaa agggttttgg accctttgtc cttatgccag cttttcttaa
    35221 ctttccacta tgataacgtt gttctagaag cgtgccgata ttcgcttcgt caaattcttt
    35281 tccagagtga tggaacactc gcacaccctt cggcaataca agtgaagcta aaccataatc
    35341 ttgcgtgatc aaaaagtcat tcttcttaac cataccgacg attttataat ccgcactatc
    35401 agctccgcgg tctacgtaga cgatttggac atgattagcg tattctttgg tcgtataatg
    35461 gtccacactg gtcacgataa ccacgtcaag accataggat tctcctaatt ttatgacatc
    35521 ttcttttact ggagaaccat ctccatcaat gaatagcctc attctttttc gccttcttcc
    35581 ataaatgata aacagttact aatccgctca gtaaaagacc aacattgatc cattttgcca
    35641 agataagcag ttgtacactt tctatcggca ctgattcatg gttcagcgtg atcaataata
    35701 gatggattgg ctctcccatt tcttttgcta aaacaaacaa aagcgtcatc aataaaaact
    35761 ggtggatcac actcccttta ttttgagtag tatacttgac aacgatccgg cgatccattg
    35821 gaatagctgt atcagtttga taatattcct tatttagcca gctgcctgtt actacgtgcc
    35881 agaaactaag cagaaagaca cctatcgcaa aaccatttgg aatcaagagg accaataaag
    35941 aagaactcag tccaagcaat tgcatagcca atggagacca tgttaaacgt ttcaaaatcc
    36001 atggtccgac tatttttgct gcaatcaagc ctgctgcata aggcaaatac ataaaaatcg
    36061 aaagtttttc taatccgtac agtatgccaa cataaaaact gctaaataaa aaaaggaaat
    36121 tccctagcat tccatttaga aaagtaaaca gatttagcca gcttggcaac ttccattcct
    36181 tcttctggtg tcctaaaaca aacacgaaca agatgaacag cagcaagaaa ccataaatag
    36241 caagatcaaa aagcctttca tcgaataaca atcgggcatt tctcaaaaga aacaataagc
    36301 caaagaatat caaaaataaa acaagctctc gcgtagctac cagatttctt ttgatgtctt
    36361 tgaaatccag ttcgtaacgc ggataatgac taaccgtatg gtaggccatg acgtaaaaaa
    36421 gtgtataaat agtaaagctt gctgcaatct gagtcggtgc aggcaactca atcgcttgat
    36481 acaggagaaa tagccagata gctgcaaata agtacttttt cccatccata ttgataaaac
    36541 cttgcgtctt ttcgtgaaaa ttgaccgtgg tattagcagg tacaagccat gctgcactca
    36601 gccctagcaa aacacttgaa aaataaaata aaggaaaata aaatacgcct aatattccaa
    36661 gcaatgaacc agcaccacca atcagcagag cgatcatcag caacgtgtaa ctatctagac
    36721 caaatcgtag actgttgact aaaaatagtc cagtcatccg aaaggcataa aacagcgtaa
    36781 atggcaaaat cgtcagccaa ttatgcgagt ctgttagtcc caaaaataaa agataaggaa
    36841 taaaaataac cgtgttactt aagataaagg gcgttgtttg tgtaaaatta cgcccaaatc
    36901 gtttgagttt ttcgaacatg acaatggctc cttaaacagt cttataacta atctgctctt
    36961 cgattttagc attaaagggg aaataaacca attttacaga atatattctt gtaaaaaaaa
    37021 atacagaaag aagcataagc ttctttctgc atttttacgg tacttgatta ctcaagcaga
    37081 caagcccttc tccacaaaag tcttgcccga cacccctgca tccggcaggt gataaagata
    37141 gaaatcaatt atcctgtgcc tccaaaatca attaggagct ctgtaaacca gagtccttct
    37201 cgacccgtcg cataaattta ctttaacaca gttcctatgt ttatgcgaga gcttttttca
    37261 ttttataatc gaccatttga aatccttgac cgatatctga aataagttct tctgtaatct
    37321 caaatccttc atgcttataa acctcgattg cttgatgatt cccctgattc actcgcaaaa
    37381 agatctctct ttcctttgtt tgcgcagcaa gttcttcata ccagcgaaaa actgaagaag
    37441 tcaatccttg accacgtact ttatttagca aatataattt acttaaataa atttttccat
    37501 ttatctcttc ataagcagtg taaccgacag tcttttcttc gctttttaat aaaaagtagg
    37561 acacaccttc tgtcagttct ttttgaatct gtttttttga ctgataagtt ttcagcatgt
    37621 aatccacttg tttttgacca atgatcggca caaaagcttc cggccatact tcttgtatga
    37681 ctgccatcag ctcatccagt tcattttctg ttacttttat tttttccata cacccacgtc
    37741 cttttttaca ttataaatga ccttgcgctt gatggaataa aaagagcatg atcatgctat
    37801 acacacccac cgaccatggt ttcaagaaaa tcacaatctg cataaattcc ctaaaagata
    37861 atttcgtcaa accagctact agacagatca agtcgtctgg cgcaaaggga agcagcaagg
    37921 taacaactag tactttttta atatctttcg tttttcttgt cagcaattca tcaaatttct
    37981 gatacttttt tggcgataaa ataagctgga caaaggctac gccatagtaa cggacaagca
    38041 aaaatccgat tgcttcccca atcactaggc caatacaact atataaaagc ccaaatccat
    38101 tgccaaacat cagcatgccc gctactgagg agatccctcc cggcaaaata ggaatgatca
    38161 cttggatcac ttgcaacaga acaaatatca tcactgcata cttgccaaat tgctggataa
    38221 acgcttgcaa agacgtgctc gatcgaaaaa tcccatgatg ataagcataa agaatcaaca
    38281 caaagaaaaa aaacagacca ataatgggca tgatctgaag acatttttgt aaaactgatc
    38341 cttttttaga catctatatt ccccacatct tttttcattt attaaaactg cctcct
//
LOCUS       DASHHB010000023        38075 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.023, whole genome shotgun sequence.
ACCESSION   DASHHB010000023 DASHHB010000000
VERSION     DASHHB010000023.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 38075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 38075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..38075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.023"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            214..498
                     /locus_tag="U2K71_001292"
     CDS             214..498
                     /locus_tag="U2K71_001292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1905 domain-containing protein"
                     /protein_id="HEM7315386.1"
                     /translation="MREIYSFESKIYASEVGKGGAYIIFPYDIRKEFGAGRVKVQATF
                     DNVPYEGSIVNMGVKNPDGSVCYILGLRKDIRKNIGKEIGDIVAVTVKQK"
     gene            complement(779..1198)
                     /locus_tag="U2K71_001293"
     CDS             complement(779..1198)
                     /locus_tag="U2K71_001293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid decarboxylase"
                     /protein_id="HEM7315387.1"
                     /translation="MEKETIVEVTKLGFTIQAAVKVIGKDLLITLTGGDTPHIGTVTT
                     FSRDTEMQTVRFPSHDGRFHKDDVLAEKIAVIIKTAVPGSCTITSGIHVDHISNAQIN
                     ASFPMSEELGYKILDWLEKIDFHIKEPVYYKDGEKPL"
     gene            complement(1200..1784)
                     /locus_tag="U2K71_001294"
     CDS             complement(1200..1784)
                     /locus_tag="U2K71_001294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiX family flavin prenyltransferase"
                     /protein_id="HEM7315388.1"
                     /translation="MEQLAQRKKKIVIGVSGASGTIYAINLVKKLKEYPMIETHGIIS
                     PWAKQNLKLESDLSLTEFESLFDYHYSNKDLGAAVASGSFLTDGMIIVPASMKTVAGI
                     SVGLGDNLIARAADVTMKEQRKLVIVPRETPLNAIHLENLTKLARLGVQIIPPIPAFY
                     NQPQTIQDLVNHHTMKLLDQFEIVNEHSKRWQGD"
     gene            complement(1831..3306)
                     /locus_tag="U2K71_001295"
     CDS             complement(1831..3306)
                     /locus_tag="U2K71_001295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UbiD family decarboxylase"
                     /protein_id="HEM7315389.1"
                     /translation="MTDQPYDLRKVIEELKTLPGQYHETDIEIDPEAELSGVYRYIGA
                     GGTVKRPTQEGPAMTFNNIKGFPGTRVNIGTMASRKRVGEILHHDYRTLGRFLKDAVE
                     NPIKPVKVDKADAPAQEVVHLATDADFDIRNILPAPTNTPEDAGPYITMGVVLGSDPE
                     KTMSDVTIHRMVLEDKDTIGMYIMPGGRHIGHFQKQFEALNKPMPITINIGLDPAIAI
                     GTTFEPPTTPLGYNELWCAGALRNEPIQIVDGIAVDEVGIARAEFIIEAEIMPNETMQ
                     EDINTNSGKAMPEFPGYTGDANPAVQVVKVKAVTHRKDNPIMQTTIGPSEEHVSMAGI
                     PTEASILALVDKAIPGKVVNVYNPPAGGGKLMTIMQIRKENEADEGIQRQAAILALSA
                     FKELKTVILVDEDVDIFDMNDVVWTFNTRFQGDKDIIVIPGMRNHPLDPSELPEYNPG
                     SIRFRGISAKTIFDGTVPFDMKEHFVRAKFQEVEDWEKYLK"
     gene            3487..3798
                     /locus_tag="U2K71_001296"
     CDS             3487..3798
                     /locus_tag="U2K71_001296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315390.1"
                     /translation="MKKMEESFPKAVKVENIANILKVTFENGEVKYVKSHWTEEITDA
                     LQFGKKGRGKRKNLLALSRNMWIGTEVTIEADGTVFINGKDRYTPEELWYKGKKSIPE
                     L"
     gene            complement(3913..6249)
                     /locus_tag="U2K71_001297"
     CDS             complement(3913..6249)
                     /locus_tag="U2K71_001297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7315391.1"
                     /translation="MLQLKDIKKYYKVGETTTKALDGVSVAFRQKEFVAILGPSGSGK
                     TTMLNVIGGLDNYDSGDMVINGKSTKDFKDSDWDAYRNNSIGFIFQSYNLIGHLGIIE
                     NVELGMTLSGVSKDEKRKRAEDALHRVGLTNHMHKKPNQLSGGQMQRVAIARALANDP
                     DILLCDEPTGALDTETSIQIMELIQELSKEKLVIMVTHNPELAHQYADRIIEFSDGKI
                     LTDSHPHIERPKDDQFNLRRTKMSFWTALKLSFNNIRTKKGRTFLTSFASSIGIIGIA
                     IVLSLSSGFQKQIDNTQAETMAKFPITISKVTTNQTRDDAGLGASKADYPDSKTITAK
                     VSEEDKAQHTNKIDQTYVDYVTDIDPNLSNNIGFTRTTGINLLRDVNGKVQPVSFSNQ
                     NPDTESLSLSSTMSAMTGVGVSSFPTQLDTSKENFLKDNYSLLAGSYPASATDVVLIV
                     DGNNNTNINALKNLGFDVKEDEKLDFDDIVGTTFKLVNNNTYYTKLPTGNFIPNTDYD
                     AMYQNASDELKISGILRVKSSSTMNLLSPGIAYSDQLTTQIVNENKESEIVKAQRDSG
                     VNVLTTEKVDENAKQTLLSYLGGDSLPSSIMIYPNNFEDKEKILDYLDDYNKGKSDED
                     KIIYTDLAGTMTELTGGLMDAITYVLIAFAGISLVTSMIMISIITYTSVIERTKEIGV
                     LKALGARKKDITRVFDAETCILGISSGILGVFIAWLATFPINSILYNMTDLKNVAQLN
                     PVHAIILVIVSTILTMLGGHLPARMAAKKDAAIALRAE"
     gene            6391..7059
                     /locus_tag="U2K71_001298"
     CDS             6391..7059
                     /locus_tag="U2K71_001298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7315392.1"
                     /translation="MNRILVVEDDPQLQLLYRSVLERAGFSVITALNGEDALKKLEAT
                     RIELIITDIMMPKMDGYDLLDSLRSTRFDTPVLMITAKADFEDKKRGFKLGADDYMTK
                     PIDVNEMILRVEALLRRSKINYSSSLIIGQTKLELDAYTVLQDQHSLILPQKEFLLLY
                     KLLSYPNKIFTRQQLMDDIWGLDTNTEERTIDVHIKRLRTRFEKTEDFQIITVRGLGY
                     KAVI"
     gene            7062..8105
                     /locus_tag="U2K71_001299"
     CDS             7062..8105
                     /locus_tag="U2K71_001299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM7315393.1"
                     /translation="MKKKLFSQLWIYFASIVFISILVTLLCFLGFIFLLSRHSISPAE
                     SAGKPTLYPLFVFAGFSMVVGTGISIFVGRRILHPISALGTNMSLVATGDFSIRMDEQ
                     QKVAEVQQLYKDFNVMVQELNSIETLRNDFVSSVSHEFKTPLATIQGYVQLLQAPNLS
                     DEERQIFLYRIIESITQLSQLTENILKLNKLENQRIQLEKKEYRLDEQIREVIVFLQP
                     KWEKEQLELDIELAAVNYTGNEEFLYQVWLNIMDNAIKYNQINGQIHIKLFETATEIV
                     LEVTDSGVGMNEETRDRMFEKFYQGDTSRQISGNGLGLSLVKKILELHDGRIDYSSIE
                     GVGTTAMIRLSKQ"
     gene            complement(8342..9223)
                     /locus_tag="U2K71_001300"
     CDS             complement(8342..9223)
                     /locus_tag="U2K71_001300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM7315394.1"
                     /translation="MDIHKFEVFLDLAETMNFTKTADRQFTTQGNISKQILSLETELD
                     VKLFERAHRKIELTEAGSLLLPYVKNVVEQYHAMQEVLETYTKDNNLSLNILTIPTMI
                     NYKGFSKITEFLKRHPEFTVQLKEVESVELTYNDANEHSDTIHFARNFQAPPSSIEWL
                     PTETDDFVAVLPKNHPLASKKKLDLSELKKENFLLLGPTTNLYQPVLDLCREAGFEPK
                     ISYKGARIDLIINMIANDMGIAIVMEKTVKNLLKENTVILPISPTTESYLAFTRKVGE
                     HSLASDTFWEYLNQEEN"
     gene            9382..10110
                     /locus_tag="U2K71_001301"
     CDS             9382..10110
                     /locus_tag="U2K71_001301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HEM7315395.1"
                     /translation="MPTGVLINVGSVLLGGLIGGLVGNKLSEHFKAQLTMVFGVCSMG
                     MGIYSIAPMKNMPAVIFALVIGTAIGLIVHLGNGINKGAALMQVPISKIFPSEKLGMT
                     HDEFISTLVTVIVLFCASGTGIYGSLDSGMTGDSTILISKSVLDFFTAAIFACNLGYV
                     VSVIAIPQFIIFYILFLLAKFIYPLTTPDMILDFKACGGFLMVATGFRMINVKMFPVA
                     DMIPAMIVIMPLSWMWTNWIMPLL"
     gene            10266..10583
                     /locus_tag="U2K71_001302"
     CDS             10266..10583
                     /locus_tag="U2K71_001302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM7315396.1"
                     /translation="MHIQIPTVLLDGTVLAALSKEDMYGYALTKIVQESLSVSESTLY
                     PVLRRLKKEGYLETYDEPFEGRNRRYYSLTPMGSRHLEEIIDEWIVFRDSLDRLLEES
                     TNE"
     gene            10576..11184
                     /locus_tag="U2K71_001303"
     CDS             10576..11184
                     /locus_tag="U2K71_001303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1700 domain-containing protein"
                     /protein_id="HEM7315397.1"
                     /translation="MNEYLNEVVDHLQEIPEDDRFDLIQYYEEYFLDSGKTLDQIIDE
                     YGTPKQFALKLKISYFSEEEQAENELVKNPKRQIRLIWLIIIGLFASPVLIPLALGFI
                     LVIGGLLVALLALIFSIYVLCISILGVGLFMIISGISVFGQSILSGLFFIGVGLFATG
                     SAVFISPLLLKGTKGLFYVMMNFVKWMGRRFITKRDLHSAGV"
     gene            11239..12030
                     /locus_tag="U2K71_001304"
     CDS             11239..12030
                     /locus_tag="U2K71_001304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4097 family beta strand repeat protein"
                     /protein_id="HEM7315398.1"
                     /translation="MLTGGLLAGATYTLGAQKSLTWDNGPRLIDMVTETKKIKDANKI
                     VIHAENQTVSVIRGLDFEIKTTYDKTEKPEIKEKNGVLTVNAVKKSTGAIISLEDTSA
                     QITIVVPADHELSELTIEGNNSAIDINEVSIKKVNTKTHDGWVSFNEFTGGTITAKDE
                     LGSLRLDEANVDNLIVTGENSNFHLEDSLISKEGKVILNGGNLSLHEMENSGYTIQTS
                     GESYIEKDYQPITNTLLEQGQKKLKITAKDTSINITTDQEDNETD"
     gene            12168..12680
                     /locus_tag="U2K71_001305"
     CDS             12168..12680
                     /locus_tag="U2K71_001305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5348 domain-containing protein"
                     /protein_id="HEM7315399.1"
                     /translation="MKVEEGQREKLKTEMERLHTYITQLSQTFYDPDKEKVMVNYPNN
                     SEGRQLEQVYHEVFKHLLTVKKELDYYSLPIIDTGILKYDGKKERFIFKSVREDLPLS
                     AGMDLEVLVEDYFTEEKHWVRTKLDYLPQAAGGTQARGWYITEDKELELEGVMARIRK
                     KIKKVTLDIY"
     gene            12706..13578
                     /locus_tag="U2K71_001306"
     CDS             12706..13578
                     /locus_tag="U2K71_001306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
                     /protein_id="HEM7315400.1"
                     /translation="MREKRAFYINGYAGIVGLVVLALVGLFFFYLGMWQAKVWALFLS
                     IFLWLITLLLLSSATVVSPNQAKVILFFGQYLGTIRENGFFLTIPLAQKMTVSLKVRN
                     FNSSVLKVNDLDGNPIEISAVVVFKVVDTAKALFDVAYYQDFVEIQSETAIRHIASQY
                     PYDTFNENDLTLRGNTTAVSDELQKELQERLAVAGVEVIETRLNHLAYATEIASAMLQ
                     RQQAKAILSARQTIVEGAVTITQMALEQIEDGQEINFTDDRKVQLINNLLVSIITDKG
                     TQPVINTGDMKDRK"
     gene            13754..14116
                     /locus_tag="U2K71_001307"
     CDS             13754..14116
                     /locus_tag="U2K71_001307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM7315401.1"
                     /translation="MKKLLIGLLVFIVVVLAGVKIADYVVMGGEEYYVQITTDGTKTV
                     SKSDSGQETVNYRYILPGYTKEGKEKELDFKGQLTRPLRKGAYLKITWNKNKGVTSYE
                     EVEKKDVPKTALEKLIKG"
     gene            14125..14877
                     /locus_tag="U2K71_001308"
     CDS             14125..14877
                     /locus_tag="U2K71_001308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315402.1"
                     /translation="MNKIVEVQNVTKVYGKNNEKKTQALSGISFDVEKGEFIGIMGAS
                     GSGKSTLLNILSTLDKPTDGHIRINQKDVTTLKGNQLADFRANEIGFIFQDFNLLESL
                     TAQENIAVPLSLQGVRPKEIKQRVQKIAERLSISHILESYPSEISGGQKQRVAAARAL
                     ITQPTILLGDEPTGALDSKSARDLLDTMDELNTKDHVSILLVTHDPFSASYCQRILFI
                     KDGGIHQEVKRGDQSRDSFYREILSILGNLEQ"
     gene            14891..16693
                     /locus_tag="U2K71_001309"
     CDS             14891..16693
                     /locus_tag="U2K71_001309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315403.1"
                     /translation="MLWKLSLTGIKSRLRDYLVLFSGLVMASAIFYMFESMASNEAFL
                     KSNTIVAATVIIFRFGSVLLGIITFVYILYANSFLMTMRQKDYAMFMMLGAKGRKIAQ
                     MIFSETFLVGIAATLVGSAVGVGLTSIVHRLLVDQLDLNISHFTPFSIHGLLITVLFF
                     AVLFLLAAVVNAFSIVKKPILALIRADQTPTRIKQHKFLFFLEVVLGIAFLGCGYYMM
                     KHVGTYQVFGIGVALVTIILGTYFIFHSVIIFFLSLLKKSEQISLKKLNNFTLSQLNF
                     RIREYTQMLSMVAMLFALALGALTVGLGFKNEIADLTENNSGYDLVMNNPRAEDQQKI
                     KELSPTLSASYTQKEDAGTIYYNASEFAAQPLIMIKHEDGTPPKITKEKVPAKKMNEL
                     TVQEELRSYELPEQQEKEIKVVSQSEFDQLNLPQSTLQLVQVKDFQANLKPIETLVAQ
                     NKMNNPSLQKVEYSNQKYEMYEVYNGLFSGFEFMGFFLGLAFLTMLASCLMFKILSGS
                     KSDIARYEMLEKIGTRRGLLKQSIRREVGVLFLAPGILGVVHVLFGLQMFGLFMQNPY
                     KDIWVPFTIFFVLYFIYYVLTTWLYTGIVLKKRL"
     gene            16970..18010
                     /locus_tag="U2K71_001310"
     CDS             16970..18010
                     /locus_tag="U2K71_001310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEM7315404.1"
                     /translation="MEINEQNFGENYHLITITNQNGTSLSVSDLGARVVHFQTIIDNE
                     KRELILGFDEAKEYLEKDPYIGASIGRMAGRITNGTFKLDDETFEVKTDPDTGHCLHG
                     GAPSFEAKQWHYTVINGENEASVIFYQTSPDMENGFPGTLDVEVRYTLTNDDTWRVTV
                     QGISDRKTVFNPTNHVYFNLTGDPAQSIDEHILWLNSRLFATLNKDTTPTGEVVSVSG
                     TAFDFQRPKKLGEVFASDFRQSMIVGGIDHPFLLKKQSGLLNAAELTSPDEKVSIQVK
                     TDASGIVIFTANFGEDGPEMRGNKLVNHGGITFETQELPGAERLESFGDVTLEPNQVR
                     EFVTEYKINIKK"
     gene            complement(18062..20206)
                     /locus_tag="U2K71_001311"
     CDS             complement(18062..20206)
                     /locus_tag="U2K71_001311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C40 family peptidase"
                     /protein_id="HEM7315405.1"
                     /translation="MKNKKTIRLLAATWLIAPSVLSTNTVFAVDNQIADQTVTTDTTT
                     SSSATSETTDSFTEDPTGTTNSTTGSTEPTEIDPADPSENEKPAETYSLTIAPSLIGK
                     IQLTASTADSPEQQPLDITESGTVEGLLAETKVTYTITPAEGEQIASVAVNGAKEVNY
                     DAKTFTVGTNNLSITAEFKAGPDTSTEKPTDPSEEVPSTPDPETPNKPDVGNNNNNNG
                     SNNSNNNNNNSNSGNSSNQTNPSGSNNNGNTQREPNHPNNGSSNGGTNNASSRPNTAN
                     QSGIENPSSASSDFVVKSPIDAVLPTNTTNVQQAIVREAFKHLGKPYVWGAKGPNTFD
                     CSGLTYYVYMKATGHYIGGWTGEQQYAGTQIPVSQAQPGDLVFWGPSSGVTHHVGIYI
                     GNGQFIHAPQPGDKVRVTSISDFTPDFAVRVNLAGLPAASGSLTGGSSILDGLDGSFH
                     FSQNQTTDQFLKKIADDAQEIGQKEGIYASVMMAQAILESGSGNSLLSSEPNHNLFGI
                     KGSYKGSSVTFNTLEQDSSGQSYQIRAQFRKYPSYKESLEDYADLIKNGLTGNPDFYK
                     PTWKSETKDYKEATKYLEGRYATDRQYSQKLNAIIEAYDLTKYDEPKKEAATTEEANA
                     EILNMSKRFDVPIKWTTDGVPITGLEFSTIKRVNNASSIFRVASIWDLWNNFTARQIP
                     ETTTRTVKAATVPVLTLLSLDKSFWLDLPLKK"
     gene            complement(20563..21936)
                     /locus_tag="U2K71_001312"
     CDS             complement(20563..21936)
                     /locus_tag="U2K71_001312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373274.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM7315406.1"
                     /translation="METTMNPEQMVLSEQIHMLIEQLGGKDNIDSVLPCATRLRIKLK
                     KQPVANAEKLKQQIPSIKYFILLDRYAEIIMDQGLAPAYKELTRQIDHSAPKEKLNPI
                     EKLFGLISEIFTPIIPLFAGSGILKGLVVLATTIGWLSETSGTYHILSAASNAVFYFL
                     PIFLAFSAAKTFKVNGYIAAVIAAALIEPNLTSLLSDSGKAATDFLGIPVVLMQYTST
                     VMPAILGIFFYSFVEKFLKKYIRENMQLVFVPLLSLVITVPLTLMVFGPAGVYLGEGL
                     AAAITWMMDINGPISGAIIAGGWNILVIFGIQWAVNPVMISNISAYGFDRIVPLTGAA
                     NFGMAGAALGVFLRSKRSKTRSISGSAFASILLAGVTEPTVYGIAIPLKKPFVAACIG
                     AAAGGAVMGFAQVKAIAFVFGSLTTLPAFISGTFFWYLAGLAVSLVVAMITTLVSGFD
                     EDLMSYE"
     gene            complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="U2K71_001313"
     CDS             complement(21936..22331)
                     /gene="rbsD"
                     /locus_tag="U2K71_001313"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HEM7315407.1"
                     /translation="MKKGKVINSDISRVIAQMGHFDKLSIGDAGMPVPFGTEKIDLAV
                     SNGLPSFLSVLENVLEELEVQSIELAEEIKTMNPEILEKIKLLLPDTPISFIPHEEMK
                     KELNNCHAFIRTGEMTPYANIILVSGVVF"
     gene            complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="U2K71_001314"
     CDS             complement(22359..23276)
                     /gene="rbsK"
                     /locus_tag="U2K71_001314"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HEM7315408.1"
                     /translation="MNTVTVIGSINMDTTLRLTNMPKPGETMHAHEIFHAGGGKGANQ
                     AVAAKRSGARTSFIGGVGADSEGQQLLDLLTKENIDTSGIAEIQGATTGQAMIMVDAA
                     GENSILIHAGANNAFHEQEVLKNKQLITNSDFIIAQFESSLDATILAFSIAKDAGKTT
                     ILNPAPARETIPTELLEKTDIIIPNETETEIITGIRVTDHNSLVAAAEKLHELGIGTV
                     IITLGSAGAFYHTEKEHGIVPAFKVDAVDTTAAGDTFIGALSSTLQPDLSNLKEAILY
                     GNLASSVAVQSYGAQPSIPYREALEQSAV"
     gene            complement(23384..24397)
                     /locus_tag="U2K71_001315"
     CDS             complement(23384..24397)
                     /locus_tag="U2K71_001315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HEM7315409.1"
                     /translation="MVKKKITIKEVAKLSGVSITTVSLILNGNTEKFSPKTVQKVLAA
                     KEELGYQPDYFARQMITKETKTIGVLVPDITNPFFSTLMRGIEDILYKQNFVTILCNA
                     DSDHQKEIEYLAELTRRGVDGFIIATSAVSTDAINENLKKQGRPFIVLDQKKSEGFSD
                     AVRTDDFRGGYLAGMHLLSLGHQTIALVYPENPPENVHARIEGFKSALDVYQIPHDQL
                     ILLPTQFSKQGGYQITAELLDSAATGVFALNDELAFGLYRGLEEAGKSIPEDYSIIGY
                     DNIDMCEYIKPKLTTIAQPIFELGQTSAKLLLDRIQFPEKEWEEKRLPVRLEKRFSTA
                     PLK"
     gene            complement(24478..25077)
                     /locus_tag="U2K71_001316"
     CDS             complement(24478..25077)
                     /locus_tag="U2K71_001316"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM7315410.1"
                     /translation="MNDLKENMIFDLSKTAFVAIDMQKEIVNNGTLSPHTAASILTNN
                     DLLVRTLKRTAALKVLVNVDISTFPYLSQQTDMGGMTASVPPEFTDLLLKDSLKDTDN
                     MLTIKKYNPSAFFGTSLDLQLRRRGIETIILSGVATTNGVYATALDAFQHGYHIVLAE
                     DACSDRDKESHQLFIKKIFPKTARVRSTKQIIEAIQQSK"
     gene            25263..25964
                     /locus_tag="U2K71_001317"
     CDS             25263..25964
                     /locus_tag="U2K71_001317"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315411.1"
                     /translation="MKYVEVKNEYKRYKMGETTITANDDISFSIEKGELVIILGPSGA
                     GKSTILNILGGMDTPDEGQIIIDDTDIAQFSDKQLTAYRRTDVGFVFQFYNLVPNLTA
                     KENVELATEVSPDALDPVEVLRQVGLAHRLNNFPSQLSGGEQQRVSIARALAKNPKLL
                     LCDEPTGALDFETGKQVLKLLQNASRQHGNTVLIITHNSALAPIADRVIHINDAKVRS
                     VELNDHPSSIDEIVW"
     gene            25974..28685
                     /locus_tag="U2K71_001318"
     CDS             25974..28685
                     /locus_tag="U2K71_001318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315412.1"
                     /translation="MKTRTYLKAGFREIRQSKGRFIAIILIVMLGTLLFVGVKTAGPV
                     MQKTMDHYVGTAGLSDLQIVSTGGLTQKDIAQAEKIPDAKVETGKQIYYSNTGKNEVI
                     QIFSYNKNNKQNKLQLTDGKLPEKPNQLVLDEKAEDEGYKIGDIYQIDSDELKEKEYT
                     ITGFVRSPLFINNLERGYANVGNGSVDYFVYLPESVFKTDIYSVLYLDFTNVKNLNTY
                     SKAYKDKMEQNQEKAEKYLQDRPEERLEELKKSANEELEPAQQKIADGKAQTTQARTR
                     LEDAKKQLAQQSATIQQLPEAQRTIAVQTLPQQEAQLAEQERQLVEKERELASAEEEL
                     NRNAEKIENLTKPSYLYNERSENVGFQEFGDLSDRIAAIANVFPVFFFFIAALITFTT
                     MTRMVEENRREIGTLKALGYTKVEIAKKYAIYASLASGIGIVLGTILGTNLLPRIIFE
                     LSNERYDIGSALIFYDWPPILQAAAAFFIAAFGAAMIVLFKDLRERPAALLQPKAPKP
                     GKRIFLEYITPLWSRLSFNQKISYRNLFRYKSRMLMAIIGIAGCAGLMVAGVGLKDSL
                     SSVSAKQFGPIIDYQAIVTLDSSTEDSRQKAADMLQKQEKVNEILAIHTQTVELRKKG
                     HASQSLTVIVPSKTADLDRFIHLTDLEENPRSLPKTGIAITQKVAELFDLSTGDHISL
                     YDEDQKEWKVEVKEIVKNYLGSFIYMSPEYYASVTDQSMMDNAFLLRTDKMTTKEEEM
                     LSEKLLATDAVTNTSFMSKQIETQEESMANLDSVVLIFVVLSGLLAFIVLYNLTNINI
                     SERVRELSTIKVLGFFDKEVTMYIVRENIIFTLLGIIGGFGVGYILTDFILQQASMEN
                     VIFPLVITWWAYVLSAGLTIVFTVIVMIVTHFKLKHIDMIDALKSNE"
     gene            29082..30710
                     /gene="treC"
                     /locus_tag="U2K71_001319"
     CDS             29082..30710
                     /gene="treC"
                     /locus_tag="U2K71_001319"
                     /EC_number="3.2.1.93"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-phosphotrehalase"
                     /protein_id="HEM7315413.1"
                     /translation="MTFKDKVIYQIYPKSYKDSNGDGIGDLKGIKEKLPYLNDLGIDM
                     IWLNPIYPSPQKDNGYDISDYTAIDPIFGTMEEFEQLIAEAKKYRIEIMLDMVLNHVS
                     TEHEWFKKALAGDQHYQDFFILREEPTDWVSKFGGSAWSPFGDTGKYYLHLYDRTQAD
                     LNWRNQAVREELFKVVRFWMEKGVKGFRFDVINVIGKDEQLKNNKENDGKPEYTDKPI
                     THTYLKELNQATFGTDSTIITVGEMSSTTIENCILYTEPSREELSMVFNFHHLKVDYE
                     NGNKWSTPPFDFAELKRLFHTWGENMSEGNGWNALFFNNHDQPRALNRFVVIDQYRKQ
                     GAEMLATMIHLNRGTPYIYMGEEIGMIDPDYSSISDYIDVESLNAYKLLLEQGFTEKE
                     AFRRIKAKSRDNSRTPMQWSDTEQAGFTTGKPWLKVGGKLEEINVEKELSSEDSILSY
                     YKKLIRLRKTYPIVAQGDYHAYEANHPEVYGFLRQFEGQQLLVLTNFYDKDTRIAIPE
                     GFTEANILIDNYSDTVLHQSLELKPYQAIALLKN"
     gene            complement(30770..31951)
                     /locus_tag="U2K71_001320"
     CDS             complement(30770..31951)
                     /locus_tag="U2K71_001320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM7315414.1"
                     /translation="MKKETDTTNRLIRFLGGKTSYYVLGIVILLALIIYFFHQISFIF
                     KPVGIIIATILPPLLFALILYYLLEPVIKRLSKHLSRNLSVIIVYVIILFLLGLGSIW
                     LIPFLEEQAKTLISSLPDLFQDFQESLRQFLEKTPFADSFNQFFASIDDVTSDIAGFI
                     GNYWESGAQRISNIFSTVTAIFITLFTGPIVAFFLLRDPQKFYQSVLAIVPPAFRTDF
                     KNLTKIANQQLGSFLKGQIIASFILGAVYWVCFLLIGLEFASVLAISAGLLCIIPYIG
                     PFIAFFPGLIIAFQDSTFMAVKFVIVWFAVQLLHGDLVIPRVMGDRLQIHPITILIVL
                     LVMGDLMGIVGVIFGIPIYTLVKLLVTFIFRKFKQRYNKFYGDKGEYENSSFSEEDYF
                     K"
     gene            32203..33726
                     /locus_tag="U2K71_001321"
     CDS             32203..33726
                     /locus_tag="U2K71_001321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000724057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein
                     AdcA"
                     /protein_id="HEM7315415.1"
                     /translation="MKRLIGILAMLVLAGMLTACGASGKAEDSKEKLSVMTTFYPMYD
                     FTKAIVGDEGEVELLIPAGTDSHDYEPSAKDMAKIQDTDIFVYNDENMETWVPAIQKT
                     LQEGNVHTIKATEGMLLLPGSEEGHDHDHEHGEEGHTHELDPHVWLAPSLAIKQVANI
                     RDQLIEAYPEKQEVWTKNAAAYTEKLQALHQLYQETFKQAKQRSFVTQHAAFNYLALE
                     YGLNQVSIAGLSSSEEPSAARIAELKHFVKEHGINYIYFEENAKDSIARTLANEAGVS
                     LEVLNPLEGLTNEQIENGENYLSIMEANLEALKKTTETENPLEDTLTPKKEKSVYNGY
                     FEDEDVQDRSLSDWAGTWQTVDTYVEDGTFDPVFEYKEKLNQDKTAQEYKEYYTKGYQ
                     TDIASIKISEKEMAFTFKDGTTKRSNYRYVGKEILTYEAGNRGVRYLFEAEDKESGAF
                     RYIQFSDHTIAPADSEHFHLYAGNESQEVLLKEMENWPTFYPDTMDGREIAQEMLLH"
     gene            complement(33795..34523)
                     /locus_tag="U2K71_001322"
     CDS             complement(33795..34523)
                     /locus_tag="U2K71_001322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7315416.1"
                     /translation="MIPIYICEDDPKQLEVLSTVIKNRIMIENLDSYVHISTSDPEEL
                     LNAAKIRTSSFGIYFLDIELEDSEMDGVAIGKLIRELDPLGKIIYVTSHTEMSMKILK
                     SNIEPTDYIVKEDLYDLKENVEHILSKIFEDFQMSQIQKDIFKIEFNDEIKFLPIKDI
                     QYFSTSLGAPHKLEVHLTHAQLQFYEKIKEIEKMHRNFIRCHKSYVINTQNVRSINKK
                     TREVTLANGETIPASIRGLKKLIS"
     gene            complement(34536..35816)
                     /locus_tag="U2K71_001323"
     CDS             complement(34536..35816)
                     /locus_tag="U2K71_001323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM7315417.1"
                     /translation="MFLWSIALQYLIYTICILLIDHHIRYKYLFAGVILISCILIGWV
                     YSILGIFTALIAIGMLMLTFFFFSTSRQQLVYALPFGLLTSLLGDHLTSIDDFFVLKS
                     SVIEPGDSLQYFHIVLSAFLSALLAFCLKKSLETWFHTVDRRMIGTIGTLILFTYYII
                     IFYTRFSGETPDVLALNTSFFILYLCVGLIILVYYWKISNKKLADQLLEQQVRMQQDY
                     IKDLEKNYQDLREFKHDYQNLLFSLHSYISEGDLEGLRSYYDQTILPTRQMMNLFPAN
                     LSMLDNMKVPEIRSLLSLKLMMAQEKGLIVQLHFPEPIELDTKHTVNLVRMLGIILDN
                     AIEGASAAKKKKIELTILKKNHSVMIRVINTTTNTLPLNQLKQKGFSTKTNPKNEGLG
                     LFILDELTKTNPYLFLETSIENQRFSQTIYIQIK"
     gene            complement(35957..36559)
                     /locus_tag="U2K71_001324"
     CDS             complement(35957..36559)
                     /locus_tag="U2K71_001324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEM7315418.1"
                     /translation="MKDKVVNKLLEALFSGDKSEEDMVYVQVKFALEVLLNNLGKLAV
                     VLVFSLVTGSWAETGITFLSYICIRRYAYGLHSDSEFVCLLWTLLYLWGVPLVMKHLQ
                     LTISFPMMVFLLISCFLLLLRYGSRGTAINPIEPEKRPPLLKKAISMFLIFSLITLFF
                     SASYFSTYLLLGIVLEIATLLPITNYLMNFGGIFHEKNNH"
     gene            36804..37010
                     /locus_tag="U2K71_001325"
     CDS             36804..37010
                     /locus_tag="U2K71_001325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315419.1"
                     /translation="MENEQLFEVGETYAFKSDLFESPIKGKIEKIYENSVMIRVMKCA
                     VEDNDTAHNLHWQLVVRKSSTIEE"
     gene            37322..38041
                     /locus_tag="U2K71_001326"
     CDS             37322..38041
                     /locus_tag="U2K71_001326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEM7315420.1"
                     /translation="MPIIVIIVAGTIGAGKSTLTEMLAQDLETKPFYENVEDNEVLPL
                     FYSNPEKYTFLLQIFFLNKRFLAIKDAFSHDDNVLDRSIYEDSMLFHLNADLGRVSEV
                     EVKQYEGLLETMLKELEEISPQKKPDLLVYIRVSFETMLARIKKRGREYEQLEQDPEL
                     YSYYKELNRRYEEWYEQFDICPKIVIDGDKYDFVADPACGEQIVQEIKMRAKKMTEEA
                     DAQSIRYTTNNPKAKGIFTNK"
BASE COUNT        12174 a         6778 c         7669 g        11454 t
ORIGIN      
        1 aggaagaggt tgtgagcctg ccgtttaact ctgagtcaca aggagcaatt tcaaaaaaca
       61 gcttctcatg tttgttgact attgtgactt gtgaccgagg agttggcagg agaacaccgt
      121 ttattcgaaa aaaggaactg cgacaagact tttgtcacag ttccttcttt tttttgctat
      181 gctatactag atgtaaaaaa taggagaaat aaaatgaggg aaatttattc ttttgaatca
      241 aaaatctatg cttccgaagt cggcaagggc ggggcatata ttatttttcc gtatgatata
      301 agaaaagaat tcggtgctgg aagagttaaa gtccaagcaa cttttgacaa tgtgccgtat
      361 gaaggcagta tcgtgaatat gggcgtgaaa aatccagatg gttcagtctg ctacatcctt
      421 ggactacgaa aagatattcg taaaaacatc ggaaaagaaa tcggtgatat tgtagcagtt
      481 acagtgaagc aaaaataaaa gaggttgtga caaaagacgt gtcacaacct cttgataaac
      541 ggtgttcaaa agccagtttc tcggtagtaa gtcaaattta tgcaaaaatg tggaaaccca
      601 tgtttacaaa atttttctta ctactcgaaa cgaagcggct ttatcacaac ctcttgataa
      661 acggcgttca atcagctgtc cctcggtatt agctcaaaaa cggcaaaata tgagaagcta
      721 tttttccatt tttttctctc atactcaggt cataacgctg atttcacaac ctcttctttc
      781 ataatggttt ttcaccatct ttataataaa ctggttcttt gatatggaaa tctatttttt
      841 ccaaccaatc taaaatcttg taacctaact cttctgacat cggaaaagag gcgttgattt
      901 gtgcattgga gatatggtca acatggatac cggaagtgat cgtacaactt cccggaacgg
      961 cagttttgat gatcacagct attttttcgg ctaagacatc gtctttatgg aaccgaccat
     1021 cgtggctagg gaaacgtaca gtttgcatct ctgtatcacg agaaaatgta gtgactgttc
     1081 cgatatgtgg tgtatctccg cctgttaaag tgatcaataa gtctttaccg attactttaa
     1141 cagcagcttg gatcgtgaaa cctagtttgg ttacttcaac tatcgtttct ttttccatat
     1201 taatcaccct gccaacgttt tgaatgctca tttacaattt caaattgatc caaaagtttc
     1261 attgtatggt gattaacaag atcttggatc gtttgaggct gattataaaa agcaggaatt
     1321 ggtggtatga tttgcacacc tagtcgtgct aatttagtca agttttccaa gtgaatggca
     1381 tttaatggcg tttcacgagg aacaatgact aactttcgtt gctccttcat tgttacatca
     1441 gcagcacgtg caatcaggtt gtcgcctaaa ccaacagaga tacctgcgac tgttttcata
     1501 cttgccggta cgatgatcat tccatcggtc aaaaatgatc cgctggcgac agcagcacct
     1561 aaatctttgt ttgaataatg gtaatcaaac aggctttcga attcagttag tgataagtcg
     1621 gattccagct ttaagttctg ttttgcccat ggactgatga ttccgtgtgt ttctatcatc
     1681 ggatattctt ttagtttctt cactaggttg atggcataaa tggtgcctga agcgccactt
     1741 acaccaatca cgattttttt cttccgttgt gccaattgtt ccattttccg cactcctttt
     1801 tatgatgtca aaacagttgc agatcattca ttatttcaaa tatttttccc aatcttctac
     1861 ttcttggaat tttgcacgta caaaatgttc tttcatatcg aaaggaactg taccgtcaaa
     1921 gatagttttt gcacttatcc cacggaaacg aatagagcca ggattatatt ctggtaattc
     1981 tgatgggtcc aatggatggt tcctcattcc tgggatcacg atgatatctt tatctccttg
     2041 gaaacgagtg ttgaaagtcc aaactacatc gttcatgtcg aaaatatcaa catcttcatc
     2101 gactaaaatg actgtcttca attctttgaa ggctgataaa gcaagaatag ctgcttgacg
     2161 ctgaatacct tcatccgctt cgttttcttt acggatttgc atgatggtca tcaatttacc
     2221 accacctgca ggaggattgt agacatttac tacttttcct ggaatagctt tatcgaccaa
     2281 ggcaagaata cttgcttctg taggaatacc ggccatagat acgtgttctt cactgggacc
     2341 aatcgttgtt tgcatgattg gattgtcttt tcggtgagta actgctttga ctttcactac
     2401 ttgtacagct ggattagcat ccccagtata gccaggaaat tctggcattg cttttcctga
     2461 gttcgtgttg atatcttctt gcattgtttc atttggcatg atttctgctt caatgatgaa
     2521 ttcagcacga gcaataccca cttcatcgac tgctatacca tcaacgattt gaataggttc
     2581 gttacgcaaa gctcctgcac accatagttc attgtagcca agtggagtgg taggtggttc
     2641 gaaagtcgtg ccaatggcaa ttgctggatc taaaccaata ttgatagtga taggcattgg
     2701 tttattcaag gcttcgaatt gtttttggaa gtgaccaatg tggcgtccgc caggcatgat
     2761 gtacataccg atcgtatctt tatcttctag aaccatacga tggatcgtta catcgctcat
     2821 tgttttttct ggatctgatc ctaagactac acccattgtg atgtatggac cagcatcttc
     2881 aggagtattt gtaggtgcag gcaaaatgtt gcgaatatcg aagtctgcat cggtagctag
     2941 atgaacgact tcttgtgctg gagcgtctgc tttgtctact ttcacaggtt tgattggatt
     3001 ttctacagcg tctttcaaga aacgaccaag tgttcgataa tcatggtgca aaatttcacc
     3061 tacacgttta cgactagcca ttgttccgat gttcacacgt gtgccgggga aacctttgat
     3121 attgttgaaa gtcatagctg gtccttcttg agtcggacgt tttactgtac cgccagcacc
     3181 gatataacgg taaacaccag ataattctgc ttcaggatcg atttcaatat ctgtttcgtg
     3241 atattgacct ggaagtgttt ttagttcttc gatgactttt cttaaatcat acggttgatc
     3301 tgtcatgaga gattcctcct tagttgtctt tacctatcta tagtaaaagg tgaaaaagag
     3361 gaaaacaatt caacttgttc ggtaaagtat tctacaatgg aatggatgcg tttttttgat
     3421 aatagaataa actatgatat acttatcact aaaagaaagc gcatacttag tgttggagga
     3481 aggaaagtga aaaaaatgga ggagtcattt cctaaagcag taaaagtgga aaatatagcg
     3541 aatattttga aagttacatt tgaaaacgga gaagtaaaat acgtgaaatc tcattggaca
     3601 gaagagataa ccgatgcctt gcaatttgga aaaaaaggaa gaggaaaaag aaaaaatctt
     3661 ctcgcacttt ctcgtaatat gtggattggg actgaagtaa cgattgaagc agatggaaca
     3721 gtattcataa atggaaaaga caggtatacg ccagaagagt tgtggtacaa aggaaagaaa
     3781 agtataccag aattataaaa aataattaaa aaaaacagcg cgaaacaagg aacatattac
     3841 ttcctcgttt cttgctgttt tctactattt cagcgctctt atgactcaaa tctaaatgtg
     3901 gttgtcacaa ccttattctg cacgcaaagc gatagcggcg tcttttttcg ctgccattcg
     3961 tgcaggaaga tgaccgccaa gcatcgtcaa tatagtagag acgatcacta gaataatcgc
     4021 atggacagga tttagttgag caacattttt tagatccgtc atgttgtata agatgctatt
     4081 gatagggaac gtagctagcc atgcaataaa cacacctaat atcccagaag aaatacctaa
     4141 aatacacgtt tctgcgtcaa atacgcgagt gatgtctttt ttacgtgcac ctaaagcttt
     4201 tagtacaccg atttcttttg tccgttcaat cacagatgta taagtaatga tactgatcat
     4261 gatcatactt gttactaaag agatccctgc aaaagcaata aggacataag tgatagcatc
     4321 catcaatccg ccagtcagtt cagtcatcgt tccagctaaa tcagtataaa tgattttatc
     4381 ttcatctgat ttgcctttat tgtaatcatc tagataatcc aagatctttt ctttgtcttc
     4441 aaaattgttc ggataaatca tgatacttga aggcaagctg tctccaccta aataactaag
     4501 caatgtttgt ttggcatttt cgtcaacttt ttcagtagtt aagacattta caccactgtc
     4561 tctttgagct ttgacgattt ctgattcttt gttttcgttg acgatttgag tcgttaattg
     4621 atcgctataa gcgattcctg gagataagag attcattgta gaagaagatt tcacgcgtag
     4681 gatccccgag atcttcaatt catcagaagc attctgatac atcgcatcat aatctgtatt
     4741 agggataaaa ttcccagttg gaagttttgt atagtaagta ttgttgttta ctaatttgaa
     4801 tgttgtacca acgatatcat caaaatcaag tttttcgtct tctttcacat caaatccaag
     4861 attcttcaat gcgttgatat tagtattatt attgccgtct acgatcagca cgacatctgt
     4921 tgctgaggct ggataacttc cggcaagtaa agaataattg tcttttaaaa agttttcttt
     4981 gctagtgtca agctgtgtag ggaaacttga cactcccaca cctgtcatcg ctgacatcgt
     5041 actagataaa gaaagagatt ccgtatcagg attttgattc gagaagctga caggctgtac
     5101 tttgccattt acatcacgta aaagattgat tcctgtagta cgggtaaatc cgatattatt
     5161 acttaagtta ggatcgatgt ctgttacgta atcgacgtat gtttgatcaa tcttattcgt
     5221 gtgctgtgct ttatcttctt cgctgacttt tgcagtaatc gttttgctgt ctggataatc
     5281 cgctttgctt gcacccaatc cggcatcgtc acgagtctgg ttagtcgtta ccttagaaat
     5341 agtaatcgga aatttcgcca tcgtttcagc ttgcgtgtta tcgatttgtt tttggaatcc
     5401 agaagaaagg gacagaacga tcgcgattcc gatgatccca atactggaag caaaggaggt
     5461 caagaaagta cgtccttttt ttgttcggat attgttgaag gaaagcttta acgctgtcca
     5521 aaaactcatc tttgttctgc gtaagttaaa ttgatcatct tttggtcgtt cgatgtgagg
     5581 atgggaatct gttaagatct taccatcaga aaattcaatg attcgatctg cgtattgatg
     5641 ggccagttca ggattatgtg tgaccatgat cactaatttt tctttcgata actcttggat
     5701 caattccatg atctggatac ttgtttctgt atctaacgct ccggttggtt catcgcacaa
     5761 aaggatatct gggtcattgg ctagtgctcg agcgatggcc actcgctgca tttgtccacc
     5821 agataactga tttggctttt tatgcatatg atttgtcaag ccgactcggt gcaatgcatc
     5881 ttctgcccgt tttcgttttt cgtctttaga aacaccactt aatgtcatcc caagttcaac
     5941 gttttcaata atgcctagat gaccaatcaa gttgtaactt tgaaaaatga aaccgataga
     6001 gttgtttcgg taggcatccc agtcactgtc cttaaagtct ttagtagact ttccgttgat
     6061 taccatatca ccggaatcat aattatctag tccaccaatc acattcagca tagtcgtttt
     6121 accagaacca ctaggaccta aaatcgctac gaattctttt tgtctgaaag cgacagacac
     6181 accatccaat gctttggtgg tggtttcgcc gactttataa tattttttta tatcttttaa
     6241 ttgaagcata atcacccttc tcccgtattg aaatattcgg tatactaata agtatatcag
     6301 aacaatgatc gtagtatacg aaataaatgt taactgaaga tgaactacaa gaaaaaatct
     6361 agaaattttt caaaaaacag agggagatca atgaatcgta ttttagtagt agaagatgat
     6421 ccgcaattac agttgcttta ccgttctgtg ttggaacgag cgggcttttc agtcatcacc
     6481 gcattgaatg gagaggatgc gctgaaaaaa ctagaagcga cacgtataga attaatcatt
     6541 acagatatca tgatgccgaa aatggatggc tatgacttgc tagactcatt acgtagcacc
     6601 agatttgaca cacctgttct aatgattacg gcaaaggccg attttgagga taagaaacgc
     6661 gggttcaaat taggagcaga tgattatatg acaaaaccca tcgatgtcaa tgagatgatc
     6721 ctacgtgtgg aagcattgct tcgacgttca aagatcaatt acagcagtag cttgataatc
     6781 ggacaaacaa aattagagct agatgcttat acagtgttac aagatcagca ttcgttgatt
     6841 ctgcctcaaa aagaatttct gcttctgtat aaattactgt cttatcccaa caagatcttc
     6901 actcgccaac agttgatgga tgatatttgg ggattagata cgaatacaga agaacgaacg
     6961 atcgatgtac atatcaaacg tttaaggacc cgttttgaaa agacggaaga ttttcaaatc
     7021 atcacagtac ggggactagg ctataaggcg gttatttaaa gatgaaaaaa aagctatttt
     7081 cacaattatg gatctatttt gctagtattg tcttcatttc gatcttagtg acactgctgt
     7141 gttttctcgg ttttattttc ttattgtctc gacattctat ctcgcctgct gaatcggctg
     7201 gaaaaccaac gttatacccg ttattcgtct ttgcggggtt cagtatggtc gtgggtacag
     7261 gaatatcgat ttttgttggg cggcgtatcc tgcatccaat cagtgcgcta gggacaaata
     7321 tgagtctggt agcaacggga gatttttcga ttcgaatgga tgaacagcag aaagtagcag
     7381 aagtacagca actgtataaa gatttcaacg ttatggtcca agaactcaat agtatcgaga
     7441 cactgaggaa tgactttgtg tccagtgtat cccatgaatt caaaacacca ctagcaacaa
     7501 tccaaggata tgttcaattg ttacaagccc caaatctctc tgacgaagaa cgccaaatat
     7561 ttttatatcg gattatcgaa agtatcacgc agctttcaca attaacagag aacatcttaa
     7621 aattgaacaa actggaaaac cagcgtatcc aactggaaaa gaaagagtat cgattagatg
     7681 aacaaatcag agaagtaatc gtttttttgc agcctaaatg ggagaaagaa cagctagaat
     7741 tggatatcga acttgctgca gtaaattaca caggcaatga agagtttttg taccaagtgt
     7801 ggctgaatat catggataac gccataaagt acaatcaaat caatggacag attcatatca
     7861 aactatttga gacagccaca gagattgtac tggaagtgac cgattcaggt gtaggaatga
     7921 atgaagagac cagagatcga atgtttgaaa aattttatca aggagataca agtcgtcaaa
     7981 tttctgggaa tgggctagga ctttcactgg ttaaaaaaat tctagaactg catgatggac
     8041 gtattgatta tagcagtatc gaaggagtag gaacgacagc aatgatacgg ttaagtaaac
     8101 aataggaaaa agaataaaac ccctaaaaat tctattgaag gtttttaggg gtttttagtc
     8161 gtttttttgt gattagggaa atgttcttac ggttcttttg gcataaaaat cgctgctttc
     8221 ttattgaact acgagaaatg ttagaggtgt cattgaaaca aggacatgat gcaagaaaga
     8281 gagcgaaagt catgcgtcgc tctctttttt tgggtggata gatggacaga aaatgtaggg
     8341 cttagttttc ttcttgattt aaatactccc aaaatgtatc tgaagccaaa gaatgctcac
     8401 cgactttacg agtaaatgca agataactct ctgttgttgg agaaataggg agaatcacag
     8461 tattttcttt taatagattc ttgactgttt tttccataac aatagcaatc cccatgtcat
     8521 ttgcaatcat attgatgatc aagtcgattc ttgctccttt ataagagatt tttggttcaa
     8581 atccagcttc gcgacacaga tctagtacag gttgatacaa gttcgtagtg gggccaagga
     8641 gcaaaaagtt ctcctttttc agttcgctta gatccaattt ctttttacta gctaaaggat
     8701 gatttttagg tagaactgct acaaaatcgt ctgtttcagt aggcaaccac tcaatcgaac
     8761 ttggtggggc ctggaaattt ctagcaaagt ggatcgtgtc actatgttca ttggcgtcat
     8821 tgtaagttaa ttcgacactt tccacttctt tcaactggac tgtaaattca ggatggcgtt
     8881 tcaaaaactc ggtgattttg gaaaaacctt tataattgat cattgtcggt atcgttaaga
     8941 tatttaatga aagattgttg tctttcgtat aggtttcaag cacttcttgc atagcatggt
     9001 attgttctac gacatttttt acatatggga gcaataagct tccagcttcg gtcaattcga
     9061 tttttctatg ggcgcgttcg aataatttga cgtctaattc tgtttctaac gaaagaatct
     9121 gtttagaaat atttccttgg gtggtgaatt gtcgatcagc agttttggtg aaattcattg
     9181 tttcggctaa atcaagaaaa acttcgaatt tatggatatc catcatattc ctcctactta
     9241 ttccaataaa gaatacttta acacaaaaaa tgaattggtg taatcttctc tgtctgttac
     9301 aatctttctt gcagcgaaag caataattac agaaattttt ctgatttttt tcgcttcgta
     9361 gaaaaagaga ggagtttttt gatgccaact ggagttttga tcaatgtagg ttccgttttg
     9421 cttggcggac taattggcgg attagtagga aataaattaa gtgaacattt taaagcacag
     9481 ctgacgatgg tttttggtgt gtgttcgatg gggatgggaa tctattccat tgcgccgatg
     9541 aaaaatatgc cggctgttat ttttgcttta gtgataggaa cagcgattgg tctgattgtt
     9601 catttaggta atgggataaa taagggggct gcgctgatgc aagtacctat ttctaaaatc
     9661 ttccctagtg aaaagcttgg tatgacccac gatgaattta tcagcacatt agtgacggtc
     9721 attgttctat tttgtgcaag tggtacgggg atctatggaa gtttagattc tgggatgaca
     9781 ggagacagta cgattttgat ttctaaatct gtccttgatt ttttcacagc agctattttt
     9841 gcgtgtaatt tagggtatgt cgtttctgtg atagcaattc cccaattcat tattttttat
     9901 attttgttct tattggcaaa atttatttat ccattgacta caccagacat gatcttagat
     9961 tttaaagctt gcggcggatt cttgatggtc gctaccggtt ttagaatgat caacgtgaaa
    10021 atgttcccag tagctgacat gatacctgca atgatcgtta tcatgccact gagctggatg
    10081 tggacaaatt ggattatgcc actgttataa taaaaatcaa agagaagcca ttttttgctg
    10141 actaaaaagg atcaaatctt ttagtcagtt tttttgtatt tgacaaactg attttgaaac
    10201 tatattatat gttgtataat atagattgaa ataaaatatt acacttgaga atagaaggtg
    10261 acgtaatgca catccagatt ccaactgttc ttcttgatgg tactgtgcta gcagcattaa
    10321 gcaaggaaga tatgtatggg tatgccttaa caaaaatcgt acaagaaagt ctctctgtca
    10381 gtgaatctac actatatccg gttttgagaa gattgaaaaa agaaggatat cttgagacct
    10441 atgatgaacc ttttgaaggc agaaaccgtc gatattactc gttgacacca atgggaagtc
    10501 gtcatttaga agaaattatt gatgaatgga ttgtttttcg agattcttta gaccggttat
    10561 tggaggagag cacaaatgaa tgaatatttg aatgaagttg tggatcatct tcaagaaatt
    10621 cctgaagacg atcgttttga tttgatccag tattatgagg aatatttttt agattctgga
    10681 aaaacattag atcagatcat tgatgaatat ggtacaccaa agcagtttgc cttaaaatta
    10741 aaaatcagtt acttttcaga agaagaacaa gcagaaaacg agttagtgaa aaatccaaaa
    10801 agacaaattc gcctgatttg gttaatcatt atcggtttat ttgcttctcc tgtcctcatt
    10861 ccactagcac tggggtttat attggttatt gggggactat tggttgcttt actagcgctc
    10921 atttttagta tctatgtttt atgtatcagt atcttaggtg ttggcctatt catgatcatc
    10981 tcaggaatca gtgtgtttgg acaatcgatt ctgagtggat tattctttat cggtgtcggt
    11041 ctgttcgcca caggaagcgc agtatttatt tcgccactgt tgctgaaagg aacaaaaggg
    11101 cttttttatg tcatgatgaa ctttgttaaa tggatgggac gacgttttat tacaaaaaga
    11161 gatctgcatt cagcgggcgt ttgaaggagg acacataaat tgagattgaa acattttttg
    11221 attaccggta ttggtctgat gcttactggt ggcctcctag caggtgcaac ttatacatta
    11281 ggcgctcaaa agagtctgac ttgggataat ggtcctcggt tgatcgatat ggttactgag
    11341 acaaaaaaaa tcaaggatgc aaacaaaatc gtcatccatg cagaaaatca aacagtttcg
    11401 gtcatacgcg gattggattt tgagataaaa acgacttatg ataagacaga aaagccagaa
    11461 ataaaggaga aaaatggcgt gttgactgtg aatgcagtca aaaaatcaac aggtgcaatc
    11521 attagtttag aagacacaag tgcacaaatc actattgttg tccctgcaga tcatgaatta
    11581 tctgaattga caatcgaagg gaataatagt gcaattgata tcaacgaagt atcaatcaaa
    11641 aaagtgaata ctaagacaca tgatggctgg gtatcgttca atgaatttac aggaggaaca
    11701 ataactgcga aagatgaatt aggctcactt cgattagatg aggccaatgt ggataacttg
    11761 atcgtcacag gtgaaaacag caacttccat ctagaagaca gtttgatcag caaagaagga
    11821 aaagtcatcc taaacggtgg caatttatct ttgcatgaga tggaaaacag tggctatact
    11881 atccaaacgt ctggtgaatc ttatattgaa aaagactacc agccaataac aaatactttg
    11941 ttagaacaag gacagaaaaa gctaaagatc actgctaaag acacatcgat caatattacg
    12001 acggatcaag aggacaatga aacagattga tcctgtactc aactggatat tttaagaaaa
    12061 aacagcaaag atccttccaa tctttgctgt ttttcatcgc ttaacgcaca taactatagt
    12121 aaaataagaa agaaaatatc taaaaagaaa acagggtgaa agagtatatg aaggtagaag
    12181 aaggtcaaag agaaaagtta aaaacagaaa tggaaagatt acatacttat atcactcaac
    12241 taagccaaac cttctatgat ccagacaaag aaaaagtgat ggttaattac ccgaacaatt
    12301 cagaaggtcg ccaattagag caagtgtacc atgaagtttt caaacattta ttgacggtga
    12361 agaaagagct ggattattat tctttaccga ttatcgatac aggaatattg aagtatgatg
    12421 ggaagaagga acgatttatc tttaaatctg ttcgggaaga tttgcctctt tcagcaggga
    12481 tggatctaga ggtcttggta gaagactatt ttacagaaga aaagcactgg gttcgaacaa
    12541 aattggatta tttaccgcag gcagccggag ggacacaggc aaggggttgg tacatcactg
    12601 aagacaaaga gttagaacta gaaggtgtaa tggcgcgtat tcgaaaaaaa attaaaaaag
    12661 taacattaga tatttattga aggataaagg agcgcaggaa gtagcatgag agaaaaaaga
    12721 gcattttata tcaatggtta tgcagggatt gtcggattgg tcgtattagc tttagttgga
    12781 ttgtttttct tctatttagg tatgtggcaa gcgaaagttt gggcactttt tttaagtatt
    12841 tttctttggt tgatcacgtt attgttgtta agttcggcaa ctgtagtcag tcctaatcaa
    12901 gcaaaagtaa ttttgttttt tggacaatat ttaggtacga ttcgagaaaa tggctttttc
    12961 cttacgatac ctttggcaca aaaaatgact gtttcgttga aagttcggaa tttcaatagt
    13021 tctgttttaa aagtcaatga tttggatgga aaccccatcg aaatctcagc agtagtagtc
    13081 tttaaagtcg ttgatacagc aaaggcatta ttcgatgtag cgtattatca agattttgta
    13141 gaaatccaaa gtgagacagc cattcggcat atcgctagcc aatacccgta tgatacattc
    13201 aatgagaatg atttgacgct tcgaggaaac acgacggcag tgtctgacga gttgcaaaaa
    13261 gagctgcaag agcgtttagc agtagcaggt gtcgaagtaa tcgaaacacg cttgaaccat
    13321 ttagcctatg caacagaaat cgctagtgcc atgctccaac gtcaacaagc aaaagccatt
    13381 ttgtctgctc gacaaaccat tgttgaagga gctgtcacaa tcactcaaat ggccttagag
    13441 caaatcgaag atggacaaga aatcaatttc accgatgatc ggaaagtcca attgatcaat
    13501 aatttacttg tctcaatcat tacagacaaa ggaacgcaac ctgttatcaa tacaggcgac
    13561 atgaaagacc gtaagtgaca tactttttgt aagataggaa gaaggagttt aggacggatt
    13621 attgtcttga actctttttt tagttgtttt aaagaaaatg acaaaattga aagaagaatg
    13681 atagttgatt cgaaggcaga attgttcgct tttttctata atcagtatat aaagtaagag
    13741 agaaggggaa aaagtgaaaa aacttctgat cggtctactt gttttcatcg ttgtggtact
    13801 tgcaggggtg aaaatcgcag attacgtagt tatgggtgga gaagaatact atgtacaaat
    13861 tacgacagat ggtacaaaaa cagtttcaaa atcagactct ggacaagaaa cagtcaacta
    13921 tagatatatc ctgcctggct atacgaaaga gggtaaggaa aaagaactgg acttcaaggg
    13981 acaattgact cgaccgcttc gtaaaggagc atatttaaaa atcacctgga acaaaaataa
    14041 aggtgtcact tcgtatgaag aagtcgaaaa aaaagacgtg ccgaaaacag cactggaaaa
    14101 actaattaaa gggtagggaa agaaatgaac aaaatagtcg aagtacagaa tgtaacaaaa
    14161 gtatacggaa aaaacaatga gaaaaagacg caagcattaa gcggcatcag cttcgatgta
    14221 gaaaaaggcg aatttattgg gatcatgggc gcttctggtt ctggtaagtc gacacttttg
    14281 aatattttat ccacattaga taaaccaaca gatgggcata tccgaatcaa tcaaaaagat
    14341 gtgaccacat taaaaggaaa tcaactagca gattttcgtg cgaatgagat tggctttatt
    14401 ttccaagatt tcaatttatt agaaagcttg acagcacaag aaaatatcgc tgttcctctt
    14461 tctttacaag gcgtcagacc aaaagaaatc aagcaacgtg tccaaaaaat tgcagaacgt
    14521 ttatccatct ctcatatttt ggaaagctat ccttctgaga tttccggtgg acaaaaacaa
    14581 cgggtcgccg ccgcacgtgc attgatcaca caaccaacga ttctattagg ggatgagcct
    14641 acaggtgcgc tggattccaa aagtgcccgg gatcttttag atacgatgga cgaattgaat
    14701 acaaaagatc atgtatcgat tcttcttgtg acgcatgatc cgttttctgc aagttattgc
    14761 caacgtattt tattcatcaa ggatggcggt atccatcaag aagtgaaacg tggagatcaa
    14821 agccgtgatt ccttctatcg agaaatacta tctattcttg gaaacttgga acagtagggg
    14881 gagattgact atgctttgga aattatcact tactgggatc aagagccgtt taagagacta
    14941 cttggtgtta ttctcaggat tagttatggc atctgctatt ttttacatgt ttgaatcaat
    15001 ggcaagcaac gaagcttttt tgaaaagtaa tacgatcgtt gcggctacag ttatcatttt
    15061 ccgattcggt tctgttttac tagggatcat cacattcgtt tatattttat atgcaaattc
    15121 ctttttgatg acgatgcggc aaaaagacta tgcaatgttt atgatgctgg gagcaaaagg
    15181 cagaaaaatc gcacagatga ttttttctga aacattctta gtagggatcg cagctacttt
    15241 agtgggttca gctgtcggtg taggattgac aagtatcgtc caccgattat tagtggatca
    15301 gttagatctc aatatttcgc attttacacc ttttagtatt cacgggttgt tgatcactgt
    15361 tttgtttttt gctgttttgt ttttattagc tgctgttgtc aatgcttttt cgattgtcaa
    15421 aaaaccgatt ttagcattga ttcgtgcgga tcagacacca acaagaatca agcagcataa
    15481 attcttgttt ttccttgaag tagtactggg aattgctttt ctaggctgcg gttactatat
    15541 gatgaaacac gtggggacct atcaagtctt tggtataggg gtagccttag ttacgatcat
    15601 attaggaact tatttcattt tccattctgt gatcattttc tttttatcat tactaaagaa
    15661 atcagaacag atttcattga aaaaattgaa caatttcaca ttgtcacagt taaactttcg
    15721 tattcgtgaa tatacacaaa tgctctcaat ggtcgctatg ctgtttgcct tggcactggg
    15781 cgctttgacg gtaggattag gttttaaaaa tgaaattgca gacttgacag aaaacaattc
    15841 tggatatgat ctagtgatga ataatcctcg ggcagaagat cagcaaaaaa tcaaagaact
    15901 ttcaccaaca ctttctgctt cttatactca aaaagaagat gcgggaacga tttactataa
    15961 tgcaagtgaa tttgcggctc aaccattgat catgataaaa catgaagatg gaacaccgcc
    16021 gaagatcacg aaagaaaaag ttcctgctaa aaaaatgaat gaactgactg tacaagagga
    16081 acttcgaagc tatgaattgc cggaacagca agaaaaagaa atcaaggtag tcagtcaatc
    16141 agaatttgat caactgaact tgccacaatc cactttacag ctagttcagg taaaagactt
    16201 ccaagcaaac ctgaaaccaa tcgaaacact tgttgctcaa aataaaatga acaatccatc
    16261 tttgcaaaaa gtagagtaca gcaaccagaa atatgaaatg tatgaagtgt ataatggtct
    16321 attctctggt tttgaattta tgggattctt cctaggttta gctttcttga ccatgcttgc
    16381 tagctgtttg atgttcaaga tcttgtcagg aagcaagagt gacattgctc gttatgaaat
    16441 gcttgaaaag ataggaacac gacgcgggtt gcttaaacaa tcgatccgta gagaagtagg
    16501 cgtgttattc ttagcaccag gaatcttagg tgtagtccat gtcttattcg gattacaaat
    16561 gtttggtcta ttcatgcaaa atccatacaa agatatttgg gttccgttta cgatcttctt
    16621 cgtattgtat ttcatctact atgtactgac gacatggtta tatactggaa tcgtattgaa
    16681 aaagagattg tgaaaaagct gtcctgcatc aagtaataag aacagccaag caaaaatagc
    16741 tcctcatatt tttcgtttgg ttgggcttat taccgcagat gcaagctttt gaacaccgtt
    16801 tgttcggaaa aagaggttgt gacaatattt ttgtcacaac ctttttttca cggtctttta
    16861 ttagaatata gtaaattttt tctcgttact cacggaataa gctgttaaga aaacggtttc
    16921 catgtatact ggaatcagtg caaatgaaag aaaagagggt gcaaggaaca tggaaatcaa
    16981 tgaacaaaat tttggcgaaa actatcactt gattacgatc acgaatcaaa atgggacatc
    17041 tttgtctgtc agtgatcttg gggcacgggt tgttcatttt caaacaataa tcgataatga
    17101 aaaacgagag ttgatcttag gttttgatga agcaaaagaa tatctagaaa aagaccctta
    17161 tatcggtgct tcaatcggac gtatggctgg acggattacg aatggcacat tcaaattaga
    17221 tgatgaaacg ttcgaagtaa aaacagaccc ggacactggt cattgtttac atggtggtgc
    17281 acctagcttt gaagccaagc agtggcatta tacagtcatc aatggtgaaa acgaagcatc
    17341 ggtcattttc tatcagacta gcccagatat ggaaaacgga tttcctggaa ctttagatgt
    17401 agaagtacgg tatacgctga cgaacgatga tacatggcga gtgacggtcc aagggatcag
    17461 tgaccgcaaa acagtattca atcctacaaa tcatgtctat ttcaatttaa ccggtgatcc
    17521 agcacagtca atcgacgaac atatactttg gttgaatagc cgattatttg ctacgctcaa
    17581 taaagataca acgcctacag gagaagtagt ttcagtaagt ggcacagcat ttgacttcca
    17641 acgtcctaaa aaattaggtg aggtctttgc cagtgatttt cgccaatcaa tgatagtggg
    17701 cggcatcgat catccatttt tgttgaaaaa acaatctggt ttactaaatg ctgcggaatt
    17761 gacaagccca gatgaaaaag tatcgatcca agtaaaaaca gatgcttcag gaatcgtgat
    17821 ttttacagca aattttggag aagatggacc ggaaatgcgc gggaataaat tagtaaatca
    17881 tggcggtatc acatttgaga ctcaagaact gccaggagcg gagcgacttg aaagttttgg
    17941 agatgtcact ctggaaccta accaagtacg cgagtttgta acggaataca aaataaatat
    18001 aaagaaatag aataaattaa aaagggagtg tgacagtgtc acatcccctt tttaatttat
    18061 tctatttctt taaaggcaga tcaagccaga atgatttgtc taaacttaac aaggttagta
    18121 caggaacagt tgcagcttta actgtacgag tagttgtttc tggtatctgt cttgccgtaa
    18181 aattgttcca caaatcccag attgaagcga cacggaagat ggaagaagcg ttgttgacgc
    18241 gttttatcgt tgaaaattct aatcctgtga ttggcacgcc atctgttgtc catttgattg
    18301 gcacatcgaa tcgcttagac atattgagga tttcagcgtt tgcttcttca gttgttgctg
    18361 cttctttttt cggttcatca tattttgtca ggtcgtacgc ttcgatgatc gcgttcaatt
    18421 tttgtgaata ttggcgatct gtagcataac gtccttccaa atatttggtt gcttctttgt
    18481 agtcttttgt ttccgacttc caagttggtt tgtagaaatc aggattgcca gtcagtccat
    18541 ttttgatcaa gtcggcatag tcttccaaac tttccttgta agaaggatat ttacggaatt
    18601 gtgcgcggat ttggtagctt tgtccggaac tatcttgttc aagtgtattg aaggtcacgc
    18661 tgcttccttt atatgatcct ttgataccaa aaaggttatg atttggttcg cttgataata
    18721 agctgtttcc agatccactt tcaaggatag cttgagccat catgactgat gcgtagattc
    18781 cttctttttg cccgatttcc tgtgcatcat ctgcaatctt tttcaagaac tgatcagttg
    18841 tttggttttg actgaaatgg aacgagccat ctaatccatc taaaatagat gagccgcctg
    18901 tcagcgaacc tgaagcagca ggtaaacctg ctaagtttac tcggacagca aaatcaggcg
    18961 tgaaatctga aatagaagtt actcgaactt tatctcctgg ttgaggcgca tggataaatt
    19021 gtccgttccc aatatagata cctacatggt gagtaacacc ggaagaaggt ccccagaaga
    19081 cgagatcgcc aggttgggcc tgacttactg gaatctgcgt acctgcatat tgctgttcgc
    19141 ctgtccagcc accgatataa tgaccggttg ctttcatata tacgtaataa gtcaaaccag
    19201 aacagtcaaa agtatttgga ccttttgctc cccatacata tggttttcca agatgcttga
    19261 atgcttctcg aacgattgct tgttggacat tcgttgtatt tgtaggcaat acagcatcaa
    19321 ttggtgattt tacaacaaaa tcagaagaag cagaagatgg attctctata ccgctttgat
    19381 ttgctgtatt tggacgagat gaagcattgt tcgttccgcc attcgatgaa ccattgtttg
    19441 gatggtttgg ttctctctga gtatttccat tgttattcga tccagatggg tttgtttgat
    19501 tcgaagaatt cccgctgttc gaattattgt tattattgtt tgaattattt gaaccattat
    19561 tattgttgtt attacccaca tctggtttgt ttggcgtttc cggatctggt gtactaggga
    19621 cttcttcact aggatcagtt ggtttctctg ttgaagtatc cggtccagct ttaaattctg
    19681 cagtaataga taaattgttc gtacctactg taaaggtctt tgcatcataa tttacttctt
    19741 tggcaccatt aacagcgact gaagcaattt gttcaccttc agctggcgta atagtgtaag
    19801 ttaccttcgt ttccgctaat aaaccttcta ccgttccact ttccgtaata tccaaaggtt
    19861 gctgttcagg ggaatccgct gtcgaagcag tcagttggat cttaccaatc aaagaaggag
    19921 caattgtaag ggaatatgtc tcggctggct tttcattttc tgatggatca gccggatcaa
    19981 tttcagttgg ctcggtcgat cctgttgttg agttagttgt tccagttggg tcttctgtaa
    20041 aagaatccgt tgtttcacta gtagctgagc tggatgtagt cgtatctgtt gtgactgttt
    20101 gatcagctat ttgattatct acggcaaata cggtattggt cgacaaaaca gacggagcaa
    20161 tcagccaagt agcagctagc aaacgtatcg tttttttatt tttcataata aactagtcct
    20221 tttcctttca ttgattttct gtcttattca gcattcatta gacatttttt ggaacagata
    20281 atcgtgtcga aaagcttttt cttttcataa atctgtctgt gtattctaaa taaaacaatt
    20341 tgtaaataag caacgagcat tcattcgttt ttctttgacg ttcattttat tgcatagacc
    20401 acctttgttt ggggataaac aagtggggat aaaaatggtt atgcatatgt aaattatacc
    20461 aaagtttgga aagtatcaca aaaatttata aaaataatga tcctaaaaag aaaaagactg
    20521 aaatatgtga taaacatctc atacttcagt ctttttcatt aactattcat aagacattag
    20581 gtcttcatca aacccgctaa cgagagtagt gatcattgct acgactagac taacagccag
    20641 tcctgctaag taccagaaaa aagtaccgga aatgaatgca ggcaatgtgg tcagactacc
    20701 aaatacaaaa gcgatcgctt tcacttgggc aaatcccatt acagctccac cagcagctgc
    20761 accaatgcaa gcagcaacaa atggtttttt caatgggatc gcaattccgt aaacagttgg
    20821 ttcggtcact cccgcaagta aaatcgaagc aaaagcagaa ccggagatcg aacgggtttt
    20881 tgaacgttta cttcgcagaa atacacctaa agctgcacca gccattccaa aattggctgc
    20941 tcctgtcaac ggaacgattc ggtcgaaccc ataagcagag atattactga tcatgacagg
    21001 gtttacagcc cattggatac caaatattac caaaatattc caaccaccag cgatgattgc
    21061 gccactgatc ggtccgttaa tatccatcat ccaagtaatt gcggcagcta aaccttcgcc
    21121 aaggtaaaca cccgcaggcc cgaataccat taacgtcaaa ggaaccgtga taactaatga
    21181 aagtaaaggt acaaatacta attgcatatt ttcgcgaata tattttttga ggaatttttc
    21241 aacaaaagaa taaaagaaaa tacctaaaat cgccggcatg acagtagacg tatactgcat
    21301 cagtactaca ggaatcccca gaaaatcagt agctgctttg ccactgtctg aaagtaaaga
    21361 ggtcagattc ggttcaatca gtgctgcagc aatgacagcc gcaatatatc cattcacttt
    21421 aaacgttttg gcagcagaaa aagcaagaaa gattggcaga aaataaaaga cggcattcga
    21481 cgcagcagac aaaatatgat aagttccgct tgtttcacta agccagccaa tcgtagtcgc
    21541 taaaacaacc agacctttta atataccaga accggcaaac agcggaatga tcggcgtaaa
    21601 gatttccgat atcaaaccaa atagtttttc gatcggattt agcttttctt tcggagctga
    21661 atgatcgatt tgacgcgtca attccttgta agcaggagcc aatccctgat ccatgattat
    21721 ttctgcatat ctatcgagta aaatgaaata cttaatggaa gggatttgct gttttaattt
    21781 ttctgcattg gctactggct gttttttcaa tttgatacga agccgagtcg cacatggaag
    21841 gacagaatca atattgtctt ttcctcccaa ctgttcaatc agcatatgaa tctgctcgga
    21901 cagtaccatc tgttcaggat tcattgtcgt ttccattaga agaccactcc actcactaat
    21961 ataatattgg cgtaaggtgt catttcgcct gtacgaataa atgcatgaca gttattcaac
    22021 tcttttttca tttcttcatg tgggataaag ctaatcggtg tatctggtaa caataatttg
    22081 attttttcca atatttccgg gttcattgtc tttatttctt ccgccaattc aatcgattgt
    22141 acctcaagtt cttctaaaac gttttctaaa acagataaaa aacttggaag accgtttgag
    22201 acagccaaat ctattttttc agtaccaaag gggacaggca tgcctgcatc cccgatactt
    22261 aatttatcaa agtgccccat ttgtgcgatt actcgtgaaa tatctgaatt gattactttt
    22321 ccttttttca tgtttgtttc ttcctctcat tgttaatttt agactgctga ttgttctaaa
    22381 gcttcgcggt aagggataga aggttgcgca ccataagact gcacagcaac agaagaggct
    22441 agatttccat aaaggatcgc ttctttcaag ttactaaggt ctggctgaag tgtgctgctt
    22501 aatgcgccaa taaatgtatc acctgcggct gttgtgtcaa cggcatccac tttaaaagca
    22561 gggacgatac cgtgttcttt ttctgtatga tagaaagcgc cggcactgcc tagtgtaatg
    22621 attactgttc caatacctaa ttcatggagt ttttcggctg ctgcgaccaa actgttatga
    22681 tctgtcactc tgattcctgt aatgatttct gtttctgttt catttggtat aatgatatct
    22741 gtcttttcaa gtagttcagt aggaatcgtt tcacgagctg gtgcaggatt caggatcgtt
    22801 gtttttcctg cgtcttttgc aatagaaaaa gctaggatcg tcgcatctaa gctactttca
    22861 aactgagcaa taataaagtc actgtttgta atcagttgct tgttttttag gacttcttgt
    22921 tcatggaaag cattatttgc ccctgcatgg atcaagatac tattttctcc tgctgcgtct
    22981 accataatca ttgcttgtcc ggttgtggcg ccttggattt cagctatccc agatgtatcg
    23041 atgttttctt ttgtcagtag atctaataac tgttgtcctt cgctgtcagc cccaacgcct
    23101 ccaataaaag atgtccgtgc gcctgaacgt ttggctgcca ctgcttggtt ggcaccttta
    23161 cctccgccag cgtgaaaaat ctcatgcgca tgcatcgttt cgcctggctt gggcatgttg
    23221 gttaaacgaa gggtagtgtc catattaata cttccaatta ctgtaaccgt gttcatttta
    23281 caaacctcca actatctcat ttattttgat gggaaaatta ttttttaaaa cgttttagta
    23341 aaacgtttta atagttcgat aaaaatatag cacaccttga aagctatttc aagggtgcag
    23401 ttgaaaatct tttttctaat cgtacaggta atcttttttc ttcccattct ttttctggga
    23461 attggatccg atctaacaat aacttggcag atgtttgtcc caattcgaag attggttgtg
    23521 cgatcgttgt cagtttcggc ttgatatact cgcacatatc tatattatca tagccaataa
    23581 tgctgtagtc ttcagggatg ctttttccag cttcctctaa tccgcgatat aatccaaaag
    23641 ctaattcatc atttaaagca aacacaccag ttgcagcaga atcaagaagt tctgctgtga
    23701 tctggtagcc accttgtttt gagaactgtg taggtaagag gatcagttgg tcgtgaggga
    23761 tttggtaaac atcaagagca gatttaaacc cctcaatccg agcgtggaca ttttccggtg
    23821 gattttcagg atagacaagg gcgatcgttt gatgtcctag actaagcaag tgcatgcccg
    23881 ctaggtaacc accgcgaaaa tcgtctgttc gtacggcatc actaaatcct tcagattttt
    23941 tctgatccaa aacaatgaag ggccggcctt gttttttcaa attttcgttg attgcatctg
    24001 tagagacagc agaagtcgcg ataatgaatc catccacacc tcgacgtgtg agctcggcta
    24061 gatattcgat ttctttttga tgatcagaat cagcattaca taatatcgtg acgaaattct
    24121 gcttatacaa gatatcctca attcctcgca tcagtgtact gaagaatgga ttcgtgatat
    24181 caggaacgag tacaccaatt gttttcgttt ccttggtaat catttgtcta gcaaaatagt
    24241 caggctgata gcctaattct tctttggcag ctaaaacttt ctgaacagtt ttcggactga
    24301 atttctccgt atttccattt aatatcaagg agacagttgt gattgaaacg ccggaaagtt
    24361 tagcgacttc tttgattgtg atcttttttt tcaccatgtt catactccta tctaaatcct
    24421 gtggttattt tcccatgaaa tcaaaaagaa gtaaatgaaa atcacgtgtg ccagtactta
    24481 tttgctttgt tggatagctt cgattatttg tttcgttgag cgtactcgtg cagttttcgg
    24541 aaatatcttt tttatgaaca attgatggga ttctttgtca cgatcactgc aagcatcttc
    24601 agcaagcacg atatggtacc cgtgttgaaa ggcatctaat gcagtagcat atacaccgtt
    24661 tgttgtcgct acaccgctta aaatgatagt ttcgattcca cgccggcgaa gttgaagatc
    24721 aagagaagta ccaaaaaaag cacttggatt gtattttttg atagtcaaca tattatctgt
    24781 atctttcaaa gaatccttca aaagtaaatc agtgaattct ggaggtacag aagcagtcat
    24841 tccacccata tcagtttgtt gactcaaata agggaaagtg gagatgtcta cgttcactaa
    24901 aactttcaag gcagcggtac gtttcaatgt tcttactaac aaatcattgt ttgtcagaat
    24961 agatgctgca gtgtgtggag agagtgtacc gttatttacg atctcttttt gcatgtcgat
    25021 cgcgacaaaa gcagtttttg ataaatcaaa aatcatattt tcctttaaat cattcatgat
    25081 gttccttctt tcaaataaca atgtttttta tgtaagtaaa tcataaatcg tttatttgtc
    25141 aactaaatta aaggattcct ttctttagtc tagagaaatt cagtggtaaa ataaataagt
    25201 gtgagaaaac gcattcgatg catagatata gcgagaaata tcgtgaaaat ggaggaatca
    25261 gagtgaagta tgtcgaagta aaaaatgaat acaaacgata caaaatggga gaaacgacca
    25321 ttacagcaaa tgacgatatt tctttttcaa ttgaaaaagg cgaattagtg attatacttg
    25381 gtccaagtgg agcaggaaag tcaacgattc ttaatatttt aggtgggatg gatacacctg
    25441 atgaaggtca gatcattatt gatgacacag atattgcgca attttcagat aaacagctta
    25501 ctgcttaccg acgaacagat gtagggttcg ttttccaatt ctacaatctc gttcctaatc
    25561 taacagcaaa agaaaatgtg gaattggcaa cggaagtttc tcctgatgcg cttgatcctg
    25621 tagaggtatt gaggcaagtg ggcctagctc atcgattgaa taactttccg tcccaattat
    25681 caggaggcga acagcagagg gtttcaattg ctcgtgcact agctaaaaat cccaaacttc
    25741 ttttatgtga tgaaccgaca ggcgcgctgg attttgaaac gggcaagcaa gtattgaaat
    25801 tattgcaaaa cgctagcaga caacacggca ataccgtttt gatcattaca cataattctg
    25861 ctttagcgcc catcgctgat agagtcattc acattaatga tgctaaagta aggtctgttg
    25921 aattaaatga tcatccttca tccattgatg agattgtatg gtagggggat tttatgaaaa
    25981 caagaacata tcttaaagca ggttttcgcg aaattcggca atctaaagga cgattcattg
    26041 caatcatcct tattgtcatg ctaggaacac ttttatttgt aggtgtcaaa actgctggac
    26101 ctgtcatgca aaaaacgatg gatcactatg ttggtacagc aggactttct gatttgcaaa
    26161 tcgtttctac tggtggcttg acacaaaaag atattgctca agcagaaaag atcccagatg
    26221 caaaagtaga aacaggaaaa cagatctatt attccaatac tggcaaaaac gaagtgattc
    26281 aaatattttc ttataacaaa aataacaagc agaacaagct acaattaact gatggaaaac
    26341 tcccggaaaa accaaaccag cttgtattgg atgaaaaagc ggaagatgaa gggtataaaa
    26401 tcggtgatat ttatcagatt gattcagatg aattgaaaga aaaagagtat accatcacgg
    26461 gctttgtccg ttctccattg ttcatcaaca atttggagag aggatacgcg aacgtcggaa
    26521 atggtagcgt cgattatttt gtctatttac ctgaatccgt ttttaaaaca gatatttatt
    26581 ctgtcttata tcttgatttt acaaacgtaa agaatttgaa tacgtacagc aaagcttaca
    26641 aagataaaat ggagcaaaat caagaaaagg cagaaaagta tttacaagat cgacccgaag
    26701 aacgcttaga agagctgaaa aaatcggcca acgaagaatt agaaccggca cagcaaaaaa
    26761 tagcggatgg caaagcacaa acaacgcaag caagaacacg gttagaagat gctaaaaaac
    26821 agttagcgca acagtcagca actatccagc agcttcctga agcgcaacga acaatcgctg
    26881 ttcaaacatt acctcaacaa gaagctcaac ttgctgaaca agaaaggcaa ttagttgaga
    26941 aagaacgtga actggcctct gcagaggaag aattgaatcg aaatgcagaa aaaattgaaa
    27001 atttaactaa accatcatac ctatacaatg aacgttcaga aaatgtcggt tttcaagaat
    27061 ttggtgatct gtctgatcgt atcgctgcga ttgccaatgt ttttcctgtt ttctttttct
    27121 ttattgccgc attgatcacc tttacgacaa tgacacgtat ggtagaagaa aaccgcaggg
    27181 aaatcgggac attaaaggcg ttaggttata cgaaagttga aatcgcaaaa aaatatgcaa
    27241 tctatgcaag tctagcctca gggataggta ttgttttagg aacgatatta ggaacgaatc
    27301 ttttgccaag gatcattttt gagctgtcta acgagcggta tgatattggg agtgcgctta
    27361 ttttttatga ctggccgccg attttacaag cagctgccgc attcttcatt gccgcatttg
    27421 gcgctgcaat gatcgtttta ttcaaagatt taagagaacg gcctgctgca ttgcttcaac
    27481 cgaaagcacc gaagccaggg aaacgcattt ttcttgaata catcactccg ctgtggtcac
    27541 gccttagctt taatcaaaaa atcagttacc gaaatctctt ccgatataag tcaagaatgc
    27601 ttatggcgat tatcggaatt gctggatgtg ccgggctgat ggttgcaggc gtgggattga
    27661 aagattctct aagttctgtt tcagccaaac aatttggacc gatcattgac tatcaagcaa
    27721 tcgttacatt ggattcatcc acggaagata gtcgtcaaaa agcagcggat atgctgcaga
    27781 agcaagaaaa agtaaatgag atcctagcta ttcatactca aaccgtagaa ttgagaaaaa
    27841 aaggacatgc ttcgcaaagt ctgacagtga tcgtaccaag taaaacagct gatttggatc
    27901 gttttatcca tttaactgat cttgaagaga acccacgctc cttgcctaaa acaggaatcg
    27961 ctattacgca aaaagtagca gaactatttg acttatctac aggagatcat atttcgcttt
    28021 atgatgaaga tcaaaaagaa tggaaagtcg aagtaaaaga aatcgtgaaa aactatctag
    28081 gtagctttat ttatatgtcc cctgaatatt atgcttcagt tactgatcaa tcgatgatgg
    28141 ataatgcatt tttgctgcgt acagataaga tgacaacaaa agaagaagaa atgctttccg
    28201 agaaactgct tgccacagac gctgtgacaa atacgtcttt catgtccaaa caaatcgaaa
    28261 cgcaagaaga atcaatggcg aacttagact cggttgtctt aatctttgtc gtattatcag
    28321 gactgctggc ttttatcgtc ttgtataatc taactaatat caatatttcc gagcgtgtac
    28381 gcgaattatc tacaataaaa gtccttggat tttttgataa agaagtcacg atgtacattg
    28441 ttcgagaaaa tatcatcttt actcttttag gaatcatcgg aggatttggt gtaggatata
    28501 tattgactga cttcatcttg caacaagcgt ctatggagaa tgtcatcttt ccattagtga
    28561 tcacatggtg ggcttatgtc ttatcagcag gattaactat cgtatttacg gttattgtga
    28621 tgattgttac ccatttcaaa ttaaagcata tcgatatgat tgatgcgttg aaatcgaatg
    28681 aataaaaaca gaggttgtga aatcagcgtt ttgagctaat aacgagggtc agctgctaat
    28741 tgaacaccat gaatcagagg ttgtaaaaaa gccgtcctgc atcgagcaaa aagaatactc
    28801 aaataaaatt agctcctcat gttttacatt tgagaagact gattgccgta gataaaagct
    28861 tttgaacacc atttatagaa gaaacaagaa tattttgcta ttttaaacaa gaggatagac
    28921 aaatacatgt ctgatctctt gttttttatt gggcaacaga cctcttagaa tgtataaaga
    28981 taaattttac aaacgctttc tgttcagtta taataataaa tgataacttg tatatataag
    29041 ctgtggtagg taataaatca gttgaatgga ggtaaatatc catgacattt aaagacaaag
    29101 tcatttacca aatctatccc aaatcttaca aagacagtaa tggtgatgga attggagatt
    29161 taaaaggaat caaagaaaaa ctcccgtatc tgaatgatct aggcatcgac atgatttggc
    29221 taaaccctat ttatccaagc cctcaaaaag acaacggtta tgatatctct gattacacag
    29281 ccatcgatcc tatctttggt acgatggagg aattcgaaca gctaatagcc gaagcaaaga
    29341 aataccggat agaaatcatg ctcgatatgg tgctgaatca cgtttcgaca gaacatgaat
    29401 ggttcaaaaa agcactagca ggggaccagc attatcagga cttcttcatt ttaagagaag
    29461 agccaacgga ttgggtatca aaatttggtg gaagtgcttg gtcgccattt ggagatacgg
    29521 ggaaatatta tttgcactta tatgatcgga cacaagctga cttgaactgg cggaaccaag
    29581 cagtaagaga ggaactattc aaagttgttc gtttttggat ggaaaaaggg gtaaaaggat
    29641 tccgtttcga tgttatcaat gttatcggta aagacgaaca attgaaaaat aataaagaaa
    29701 atgatggcaa acctgaatat acggacaaac caatcaccca tacgtatcta aaagaattga
    29761 accaagcaac gtttgggacg gattccacta tcatcacagt cggtgaaatg agctctacga
    29821 cgatcgaaaa ttgtatcttg tatacagaac ctagtagaga agaattatca atggtcttta
    29881 atttccatca tttgaaagtc gattatgaaa atgggaataa atggtctact cctccatttg
    29941 attttgcaga attaaagcga ctatttcata catggggaga aaatatgagt gagggtaacg
    30001 gctggaatgc tctatttttt aataatcatg atcagccacg tgcgctcaat cgatttgtag
    30061 ttatcgatca gtatcgaaaa caaggagcag agatgctagc aaccatgatc catttgaacc
    30121 gtgggacgcc ctatatttat atgggagaag aaatcggcat gatcgaccca gactattcat
    30181 cgatttccga ctatattgac gtagaaagtt tgaatgctta taagttactc cttgaacaag
    30241 gattcacaga aaaagaagcg tttcgtcgga tcaaagcaaa atctcgcgat aactctcgaa
    30301 cacccatgca atggtcagac acagaacaag caggatttac tacagggaaa ccttggttaa
    30361 aagttggagg caagctagaa gagattaatg tagaaaaaga actatcttct gaagattcta
    30421 ttttgtccta ttacaagaaa ttgattcggc tgagaaaaac atatccaatc gttgcacaag
    30481 gagattatca tgcttatgaa gcaaatcatc cggaagttta tggctttctt cgccaatttg
    30541 aaggacagca gctgcttgta ttaacaaatt tctatgataa agatacaagg atcgccatcc
    30601 cagaaggatt cacagaagcc aatattctta tcgataatta ttccgatact gttcttcatc
    30661 aaagtctgga gctaaaacca taccaggcaa ttgctttgct gaaaaattaa aacaaaaaca
    30721 caaaacgtcg ttcctacgag ctattctcgt aagatcgacg ttttgtgttt tatttaaaat
    30781 aatcttcttc agaaaatgaa gagttttcat actctccttt atcgccataa aatttattgt
    30841 agcgttgttt gaatttacgg aaaataaaag taacgagtaa tttgactaac gtatagatag
    30901 gaataccaaa aataacgccc acaattccca ttagatcacc catgacaagt aaaacgatta
    30961 agatcgtaat cggatggatc tgtaaacgat cacccattac tcgaggaatc accaagtcac
    31021 cgtggagaag ttgtacggca aaccagacaa tgacaaattt gacagccata aaagtagagt
    31081 cttggaatgc aatgatcaaa cctggaaaga aggcgataaa cggcccaata tatggaatga
    31141 tacataacaa cccagcagaa atagctaaaa cactagcaaa ttctaatccg atcagtaaga
    31201 aacaaaccca gtagactgct ccaaggataa atgaagcaat gatttgcccc tttagaaatg
    31261 aacccaattg ttgattagca atctttgtca aatttttaaa atctgtacga aatgctggtg
    31321 ggacaatcgc taaaactgac tgataaaatt tttgaggatc tctcaaaagg aaaaaagcga
    31381 caattggacc agtaaataaa gtaataaaaa tagctgtaac agttgaaaaa atgttgctga
    31441 tccgttgtgc gccactttcc caataatttc caataaatcc agctatatca gacgtcacgt
    31501 cgtcaataga agcaaaaaat tgattaaatg aatcggcaaa aggtgttttc tccaaaaatt
    31561 gtcggagact ctcttgaaaa tcttgaaaca aatctggcaa actactgatc aatgtctttg
    31621 cctgttcttc aagaaaagga ataagccata tgcttccaag ccccaataaa aataaaataa
    31681 tcacataaac gataatgact gacaagttcc tcgaaaggtg cttgctcaaa cgtttgatga
    31741 ctggttccaa aagataatac aagatcagtg caaacaataa tgggggcaag atagtagcga
    31801 taatgattcc gacaggcttg aatataaacg agatctgatg gaaaaaataa atgatcagcg
    31861 caagtaagat aactattcct aaaacatagt aagaggtttt tcctcctaaa aagcggatca
    31921 aacgattcgt tgtatctgtt tcttttttca ctctttttcc tcctttatct ataacttcat
    31981 tatagacaaa agcgggtata aaaggaaaat gatagaggtt gattgcaaaa atatcgtcaa
    32041 tcattcatat aaactttctc atcaattaaa ataagaaaac gcttgacagt tcttttaacc
    32101 atatgataaa ttaaatcctg ttgctaaaac gtaactatta cggtttgcga aaagcgaatc
    32161 ggaacattca aatgaaagaa gatgagaaga ggagtgtcat aaatgaaacg attgataggt
    32221 atattggcca tgctggtttt agcaggtatg ttaactgctt gcggggcaag tgggaaagcc
    32281 gaggacagta aagaaaaatt gagcgtgatg acgacctttt atcccatgta tgattttaca
    32341 aaagcaatcg tcggggatga aggagaagta gagttattga ttccagcagg gactgattcc
    32401 cacgattatg aaccttctgc taaagacatg gcaaagatcc aagatacaga tatctttgtt
    32461 tataatgatg aaaacatgga aacatgggtc cccgcaatcc aaaaaacatt acaagaaggc
    32521 aatgtccata cgataaaagc gacagaaggt atgcttttac ttccgggaag tgaagaagga
    32581 catgatcatg accatgaaca cggggaagaa ggccatacac atgagctaga cccacatgta
    32641 tggctagctc caagtttagc aatcaagcag gtagcaaaca tacgtgatca gctgattgaa
    32701 gcttacccag aaaaacagga agtatggaca aaaaatgcag cagcctatac cgagaaactt
    32761 caagcactcc atcagcttta tcaagaaacc tttaagcaag caaaacaaag atcttttgtc
    32821 acgcaacatg ctgcattcaa ctatctagcg ttagaatatg gattgaatca agtatcgatt
    32881 gctggtcttt cttctagtga agaaccgtct gcggcgagaa ttgctgaact gaaacacttt
    32941 gttaaagaac atggaatcaa ctatatttat tttgaagaaa acgccaaaga tagtattgcc
    33001 agaacgttag caaacgaagc aggtgtatct ttagaagtat tgaatccttt ggaaggattg
    33061 acgaatgaac aaatagaaaa tggagaaaac tatctttcta tcatggaagc aaacctcgaa
    33121 gcgttgaaaa aaacgacaga gacagaaaac ccgctagaag atactttgac ccccaaaaaa
    33181 gaaaagagcg tctacaacgg ttattttgaa gacgaggatg tacaagatcg ctctctttca
    33241 gattgggcag gaacttggca aacagtagat acgtatgtag aagatgggac ttttgatcct
    33301 gtttttgaat ataaagaaaa attgaatcag gataagacag cacaagaata taaagaatat
    33361 tatacgaagg gctatcaaac agatattgct tcaatcaaga tcagtgagaa agagatggca
    33421 tttacgttta aggatggaac aacgaaacgt tcaaattatc gttatgtagg gaaagaaatc
    33481 ttgacctacg aggcaggaaa ccgtggcgta aggtatctat tcgaagcaga agataaggaa
    33541 agtggcgctt ttagatatat tcaattcagt gatcacacga ttgcaccggc tgactccgaa
    33601 cattttcatt tatatgcagg aaatgaaagt caagaagtat tgctgaaaga aatggagaat
    33661 tggccaacat tttatccaga tacaatggat ggacgtgaaa ttgcacagga aatgcttttg
    33721 cactgatagg caaaaaagaa ccgaagggtt tggatcggct ggtcatttag tcggtccaaa
    33781 ctattcggtt ctttttatga aatcaatttc tttaatccac ggatagaggc tgggatggtc
    33841 tcgccgtttg ccaacgtgac ttctctggtt tttttattaa tcgaccgcac attttgtgta
    33901 ttgataacat aggatttatg acaacggata aaattccgat gcattttctc gatttctttg
    33961 atcttctcat agaattgtaa ttgggcatga gtcaaatgga cttccagttt atgtggcgct
    34021 cctagcgaag tagaaaaata ttggatgtct ttgatcggta aaaatttgat ctcatcatta
    34081 aactctattt tgaagatatc tttttggatt tgggacattt gaaaatcttc gaaaattttg
    34141 ctcaggatat gctcgacatt ttcttttaaa tcataaagat cttctttgac gatataatca
    34201 gtcggttcga tgttgctttt taagattttc atcgacattt ccgtatggga agtcacatag
    34261 attatttttc ctaaaggatc taattcacgg atcaatttac cgatcgctac accatccatt
    34321 tcactgtctt ctagttcgat atctaaaaag taaataccaa aagaagaagt tctgatcttg
    34381 gctgcattga gaagttcttc cggatcagag gtagaaatat ggacataact gtctaagttt
    34441 tcaatcatga ttctattttt gatcaccgtt gataaaactt ctaattgttt tggatcgtct
    34501 tcacaaatat aaatgggaat catatcatta ctcctttatt ttatttgtat ataaatcgtt
    34561 tgagaaaaac gctggttttc aattgatgtt tcaaggaaaa gataaggatt ggtctttgtc
    34621 aattcatcca agataaacaa gccaaggcct tcgttttttg gatttgtttt tgttgagaac
    34681 cctttttgtt tcaactggtt taacggtaat gtatttgttg ttgtattgat cacacgaatc
    34741 atcacggaat gattcttctt gagtatggtc aattcgattt ttttcttctt tgcagcacta
    34801 gctccttcaa tcgcattgtc caagataata ccaagcatac gtacaaggtt taccgtatgt
    34861 ttcgtatcca gttcgattgg ctcaggaaaa tgaagctgga cgatcaaccc tttttcctgt
    34921 gccatcatga gttttaatga cagtaaacta cggatctcag gaactttcat attatccaac
    34981 atacttagat tggccggaaa aaggttcatc atctggcgtg taggtaatat cgtttgatca
    35041 taataagacc gtaatccttc taagtcgcct tctgaaatat agctgtgaag agaaaaaagc
    35101 agattttggt aatcatgttt gaattctctc aagtcttgat agtttttttc tagatctttg
    35161 atataatcct gttgcatcct gacttgttgc tctaataact gatcagctaa tttcttatta
    35221 gagattttcc aataataaac caagatgatc aggccaacac acagatatag aatgaaaaaa
    35281 ctcgtattca aagctaagac atcaggtgtt tcaccagaaa aacgggtata aaaaataatg
    35341 atatagtatg taaataatat caatgtcccg atcgtaccga tcatacggcg gtccacagtg
    35401 tgaaaccaag tctctaacga tttttttaaa caaaaagcta ataaagcaga taaaaaagcg
    35461 gataagacaa tgtgaaaata ctgcaatgaa tctcctggtt caataaccga agatttcaaa
    35521 acaaaaaaat cgtcgatgct tgttaagtga tctcctaaaa gcgacgtcaa taacccaaaa
    35581 ggaagcgcgt aaaccaattg ttgacgcgaa gtcgaaaaga agaaaaaagt aagcatcagc
    35641 attcctatag caattagagc agtaaaaatc cctaaaatcg aatagaccca tccaatcaaa
    35701 atacaagaaa tcagaatgac tcccgcaaac agatatttgt atcggatgtg atggtcaatc
    35761 aataagatac aaattgtgta aatcaaatat tgcaacgcta tggaccataa aaacataaag
    35821 tacagctccc attgattttt accaaattat agcacaatta gcgtattatt tttttaattt
    35881 ttcagggatt ttgaattcag aactcatacc aaagcagcag ccagatacaa aaaagtcaga
    35941 taagttagtt aattgtttaa tgattgtttt tttcatgaaa gattcctcca aaattcatca
    36001 agtagtttgt aataggaagc aacgtagcta tttctaaaac aatcccaagt agcaaatagg
    36061 tagaaaagta agaagctgag aaaaataaag taatgagaga aaaaattaga aacatgctga
    36121 tcgccttctt taataatggg ggacgtttct cgggttcaat gggattgatt gctgtacctc
    36181 ttgatccata gcgaagcagt aacaaaaagc agctgattaa caaaaagacc atcattggga
    36241 aggagatagt caattgcaga tgtttcatca ccaagggaac gccccataaa taaagtagtg
    36301 tccacagcag acaaacgaat tcagagtcgg aatgcaatcc ataagcatat ctgcggatgc
    36361 agatataaga aagaaaggtg attcctgttt ccgcccatga gcctgtcacc agagaaaaga
    36421 ctagaacgac cgctagtttt ccaagattat tcaataaaac ttctaaggca aattttactt
    36481 ggacatacac catatcttct tccgatttgt ctccgctgaa taaggcttct aataatttgt
    36541 tcacaacttt atctttcatc tcatacgtac ctccaccaca aattttatgc taaaaaaaat
    36601 aaaaaacgtc ttttgtttca taaaaagtgg ttttgacatc aaaaccgtca tgatctccta
    36661 aaaaaatctt cgttttccaa gaaccttacc aaaaaaagtg acagttttga tacgaaaagt
    36721 actttttatg acaagtttgt gctttttcga gtgatttatg ttaaaaaatt actgacctta
    36781 taaaaggtga tggagggatt cttatggaaa atgaacaatt gtttgaagtc ggcgaaacat
    36841 atgcatttaa atctgattta tttgaatcac caataaaagg aaaaatagaa aaaatctatg
    36901 aaaatagtgt gatgatacgt gtcatgaaat gcgcagtaga agacaatgat accgctcata
    36961 atctgcactg gcagttagtt gtacgcaaat caagtacaat agaagaataa aaacagataa
    37021 aatgttgaga gcataaagag gttgtgagtc tcccgtttag ctctgagtta caaggaataa
    37081 tgttaaaaaa cagcttttca tgttttatga atattgcctt gtgaccgagg tgttggcagg
    37141 agaatactgt ttatttgaaa aaagggctgc gacaagactt ttgtcacagc ctttattttc
    37201 atgaatagga gttacttttg gctactttta gagaagtagc ttttaaggag cagataaagt
    37261 caaggctttt atttctataa atgaagtagt aaaatgaaga acagatttta ttacaggaag
    37321 gatgccgata attgtgatca ttgttgcagg aacgatcggt gcagggaaaa gcacactaac
    37381 tgaaatgctt gcccaagatt tagaaacaaa acctttttat gagaacgtgg aagataatga
    37441 agtattgccg ctattttatt ctaatccaga aaagtatact tttttattac agattttctt
    37501 tttgaacaaa cgctttttgg caatcaaaga tgctttcagc catgatgaca acgtactaga
    37561 ccgctccatc tacgaagata gtatgctgtt tcatttgaat gcagatttag ggcgtgtttc
    37621 tgaagtagaa gtaaagcagt atgaaggtct tttagaaacg atgctgaagg agttagaaga
    37681 aatttcgcca caaaagaaac cagatctact ggtttatatt cgtgtatcct ttgaaactat
    37741 gttagcgaga atcaaaaaaa gaggccggga atatgaacag ctagaacaag atcctgaact
    37801 ctattcctac tataaagaac tcaatcgacg atacgaagag tggtatgaac agtttgatat
    37861 atgtcccaaa atagtgatcg atggggacaa atatgatttc gtagccgatc cggcatgtgg
    37921 agaacaaatc gtacaagaaa tcaagatgcg ggcaaagaaa atgacagaag aggcagatgc
    37981 ccagtcgatc cgctacacta caaataaccc gaaagcaaag ggaatcttta caaataaata
    38041 atataaaaac taagcaaatt gctcactcgc agttt
//
LOCUS       DASHHB010000024        37779 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.024, whole genome shotgun sequence.
ACCESSION   DASHHB010000024 DASHHB010000000
VERSION     DASHHB010000024.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.024"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            174..518
                     /locus_tag="U2K71_001327"
     CDS             174..518
                     /locus_tag="U2K71_001327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HEM7315421.1"
                     /translation="MAEKQTAGRDQLGDFAPKFAELNDDVLFGQVWSREELSAHQRSI
                     ITISALISGGNFEQLPAHLKIGKQNGITSEEIAEIITHLAFYVGWPKAWSAFSLAKEI
                     YLEENSEDKKEA"
     gene            519..938
                     /locus_tag="U2K71_001328"
     CDS             519..938
                     /locus_tag="U2K71_001328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HEM7315422.1"
                     /translation="MTTKHEEIHNGVIFPAGEKNEAYAQVFTGQSYLNMLVNDPEVSV
                     GVGNVIFEPGCRNHWHVHKDGYQILLVTGGEGWYQEEGKPARRLMAGDVVVTHKGIKH
                     WHGATKDSWFEHLAITAGNSEFLEPVTDEVYEGLENQ"
     gene            complement(1030..1380)
                     /locus_tag="U2K71_001329"
     CDS             complement(1030..1380)
                     /locus_tag="U2K71_001329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315423.1"
                     /translation="MEKELSKRIIDELLTYLYSHEITEIRIGVEFAEEGLFIEIQGTA
                     ETQPEDLDVLADLLNNPRDPSMEIYYQELLGNPRHDREDYHLLGSLIDDVEIMYAQPI
                     INIKIFRRASHSYY"
     gene            complement(1346..2677)
                     /locus_tag="U2K71_001330"
     CDS             complement(1346..2677)
                     /locus_tag="U2K71_001330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1576 domain-containing protein"
                     /protein_id="HEM7315424.1"
                     /translation="MENETDVIYIHPQKRIVSQKRKYFYLGFTGVFFLFIGLLSNTPT
                     DNWSGLLTILTSPSNLLTDYFALGGFGSAFINVGILTLLSVLLAYRHKVILNGPLFAS
                     ILTVTGFSFFGKNLYNSISIILGVYLYAVFVNKPFSQYIMIGLFGSALSPVVSYITFG
                     MRFPLLIGILLGNLAGIAIGLLLPPLAAQTLVFHRGFTLYNIGFTSGLIAMTFTAVLR
                     LFSYSIVENTLVFNEYHFPLIWIIFGFFSLTVGIGFYYNSFRLLGIREIFDSSGKLTT
                     DFIANSGIGATLINIGLVGLMLSSYVLLVGGQLNGPVIGAILSAVGFSAFGCHLKNSF
                     PILVGIFIASLFGTFHEITSTGMLVAAVFGTGLAPISGFYGSFYGVIAGVLHIALVHN
                     VSTLHGGLNLYNSGFSTGFVAGILVPILDNFTAVRKEKKTLGKRIIKKNHR"
     gene            2933..3499
                     /locus_tag="U2K71_001331"
     CDS             2933..3499
                     /locus_tag="U2K71_001331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7315425.1"
                     /translation="MRRKVYTKDQILKAAYDVVAKEGFKGFTARNIAKKMGISTQPIY
                     LEFKNMEDLKNTLLDSIFEQLKEDVFPIERTGDKIIDLGLNYIYFAKKHHSLYMALYI
                     DEHGDGKKMHDFSYNYFREIVKEDERYNQLSDEYVDALHTGTWVAVTGLASLMSSNIM
                     HPTEEQIIALIKRTIDSILALDNPEIIR"
     gene            3644..3731
                     /locus_tag="U2K71_001332"
     tRNA            3644..3731
                     /locus_tag="U2K71_001332"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3676..3678,aa:Ser,seq:cga)
     gene            complement(3783..4988)
                     /locus_tag="U2K71_001333"
     CDS             complement(3783..4988)
                     /locus_tag="U2K71_001333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM7315426.1"
                     /translation="MAKKEIDKRIKKHLTKKGEEKYQFSLYLGVDPLTGKKRKTTRRG
                     FSTPLAAERALKRLEADIQEKGLQASTAQKSKKFEYVYGLWFENYKKTVKESTWSSTK
                     QIFDTHILKVFGDKFIDKIDVFFCQEAVNTWSDSHPKIFKKIKNYTSNVFDYAASLQI
                     ITSNPMKLVSIPRGEALDIEDKNIEFYTKEELIKFLEAIRNDDDERYLFFSLLAFTGI
                     RKGEAFALTWSDIDFKSKTLNINKTVTRGYQGRLIVNTPKSKSGKRKIYLDNDLISLL
                     RKYYTKNKTIVTIQRENLIFHHDGLLYNPTVSRSWLNVIYKHHPELTKRITTHGFRHT
                     HASLLFESGASLKDVQERLGHADIQTTSNIYTHVTETQNKKVINNFVAFMKNSTQGES
                     KGESKNILG"
     gene            complement(5126..6014)
                     /locus_tag="U2K71_001334"
                     /pseudo
     CDS             complement(5126..6014)
                     /locus_tag="U2K71_001334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317363.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
     gene            complement(6101..6301)
                     /locus_tag="U2K71_001335"
     CDS             complement(6101..6301)
                     /locus_tag="U2K71_001335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315427.1"
                     /translation="MKIGMRKPSIKKSISARTTGKAKRKVKKAVIPGYGKKGSGWIKD
                     PKKAAYNKVYKKTTFSFWDLFK"
     gene            complement(6330..6752)
                     /locus_tag="U2K71_001336"
     CDS             complement(6330..6752)
                     /locus_tag="U2K71_001336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM7315428.1"
                     /translation="MYLPQIDNKINKLVKLYQTRNPFRIARELKILVLEEDLGEIYGY
                     YSKFKQIKMIHINSSLDEMGKWITCSHELGHCVLHPNENTPMLSKKTLVSELKIEKEA
                     NYFATHLTIDPTLDGFDHMTKYEKLVCFGLPDEFDRFL"
     gene            complement(6769..7191)
                     /locus_tag="U2K71_001337"
     CDS             complement(6769..7191)
                     /locus_tag="U2K71_001337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315429.1"
                     /translation="MTTFERIKELAKNKNKNLQQVAKDLKYGENYFYSLNAGKQPTAE
                     KLQEIADYFDVSVDYLLGRTEKKRYYDLTEKDERDIQKELQSLIDDLSNADGMAFSKK
                     DNEMSEATREALIISLENALRIAKIEAKKKYTPKKYRD"
     gene            7482..7673
                     /locus_tag="U2K71_001338"
     CDS             7482..7673
                     /locus_tag="U2K71_001338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315430.1"
                     /translation="MSNLDNGRAAIKEFMKENGITMDDLATAYGHTRVRMQQILDGHW
                     SGPKANRTILEIIRDYRIR"
     gene            7684..7950
                     /locus_tag="U2K71_001339"
     CDS             7684..7950
                     /locus_tag="U2K71_001339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315431.1"
                     /translation="MNILSEEFLTRLRIAIVEVVKDALSQLSKKNLSETRYLKKIEAR
                     KYVGGVNDQGFEKLIAHGLKEIRIDGFLRYDKKDIDELMAKYKI"
     gene            7962..8132
                     /locus_tag="U2K71_001340"
     CDS             7962..8132
                     /locus_tag="U2K71_001340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315432.1"
                     /translation="MGKFNRTLVFSAPLIIYALGLWGSRQALIGTIVYMLWIFIGLDE
                     AEYRAKKPVGRD"
     gene            complement(8191..8670)
                     /locus_tag="U2K71_001341"
     CDS             complement(8191..8670)
                     /locus_tag="U2K71_001341"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315433.1"
                     /translation="MSRKIDNIILLTTIFYLVIGATLSLLSFLSNSSLIVLNQKSILT
                     EFAHIDYFSFFKTGLICLFLSSFILYIGTTFCLMVLNLKMFIIEKWIVIIAKIFSSIS
                     IIIPMTLTFSKEQFNVATTFVSFIALFSFAVPKTFQSKSSNAKVNVENTDRKNDESY"
     gene            8715..9422
                     /locus_tag="U2K71_001342"
     CDS             8715..9422
                     /locus_tag="U2K71_001342"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rha family transcriptional regulator"
                     /protein_id="HEM7315434.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFDKKHRHVLTAIDELKEGVAEN
                     WADLFWEDTYVHPQNKQSYRIIYMNRDGFSLLAMGFTGKKALSFKLQYIEAFNKMEKE
                     LKDQLPSIPATKREMALLALAANEETNERVDAIESDLNDLKNNQLLAEPDYRTISNMV
                     RNKIRVICNQQHLNSKAKAELFKDLNGGIKRITGAVARNRIKAKQFDDVIDFINNWMP
                     STATMTIIKQMELLEDE"
     gene            9601..9942
                     /locus_tag="U2K71_001343"
     CDS             9601..9942
                     /locus_tag="U2K71_001343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315435.1"
                     /translation="MKKELSTLDQYLTDPSWGKSNIKETSNRKIRRNLLTDEELACDQ
                     DDLGNFVSIWDHVYLIHLSRKSKKTEYIYVIEDGLIDALEEYDRDNLIDISYYGSGKK
                     YIAEMEAEFDE"
     gene            9935..10681
                     /locus_tag="U2K71_001344"
     CDS             9935..10681
                     /locus_tag="U2K71_001344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEM7315436.1"
                     /translation="MSEKEQPLKNRSDNTLFNTLYKINVKDVTEKRNNLTYLSWAWAW
                     AEVSKVCEAVDYEIYHDPETYLPYVFDKKTGYMVFTSITVNGVKRDMWLPVMDGANKA
                     MKDEPYTYEVNDYQWNNETKKKEIVGKIEKRVEAATMFDINKTIMRCLVKNLAMFGLG
                     LYIFAGEDMPEDVSMLEPATQRSKKLFLDALQLVANKYEKSIDEAIVALTDAASITAD
                     DSKWTKRDLGILKRGVNWIEDQYREETKEK"
     gene            10687..11373
                     /locus_tag="U2K71_001345"
     CDS             10687..11373
                     /locus_tag="U2K71_001345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315437.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDIGATINEEVNIDRLKTMYDGYDGD
                     RIIEVRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHNIGVINFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            11380..12213
                     /locus_tag="U2K71_001346"
     CDS             11380..12213
                     /locus_tag="U2K71_001346"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage replisome organizer N-terminal
                     domain-containing protein"
                     /protein_id="HEM7315438.1"
                     /translation="MSDNKRYYYLKLKENFFDSDEMVLLESMPDGYIYSNILLKLYLR
                     SLKHEGKLMFNDRIPFNSTMLATITRHSVGVVEKAVQIFRDLQLIDVLDNGAIYMSDI
                     QSFIGKSSTEADRKREYRKKIEEAKRNLITGGQVSDKCPDKTTPELEIEIEKDIDIDK
                     EEKKGKYSDEHLRLAKKLQSNLTEDFPKEMNKVDIEKWADTIRLMEERDKASIEAIEY
                     VINWLPTNEFWFGNIRSAKKLREKFEKLKFEIKADKNNHKKQSQKLQYSNPSEYDDLP
                     I"
     gene            12230..13081
                     /locus_tag="U2K71_001347"
     CDS             12230..13081
                     /locus_tag="U2K71_001347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM7315439.1"
                     /translation="MESLANAMEKLIRRVLVQSGKCPECSEPLYSWRAKNKDGSERCK
                     PTCMSCGYKALRVKEDIQTERIYNDSLKARALSFFQNGSVLTDKTLFKCKMENYHVVD
                     QETKIALERAKSFVNDVLLNHPAHFILSGKSGSGKSHLSMATAWEILERSNYDKKILF
                     ISYQELLEQIKFSYNNAELRKEIEGSLIADIKTTDLVVFDDIGAELGSGVSNSRQFTN
                     NTLNTLLEARQNKATIITTNLSGPELREAYGERIVSRIFKNSEGYALKFQQTADKRIK
                     PVKGSIA"
     gene            13078..13437
                     /locus_tag="U2K71_001348"
     CDS             13078..13437
                     /locus_tag="U2K71_001348"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1064 domain-containing protein"
                     /protein_id="HEM7315440.1"
                     /translation="MNKYRNKKTVHRGIKFDSIAEAEYYDLALWQAEANGWKVKLQER
                     FELMPKFELDGKKYRKIEYIPDFTFYKNGKLVKVVDVKGMQTKDFKIKAKLFCHQYQV
                     PLILAKKYRNTFKEERF"
     gene            13452..13613
                     /locus_tag="U2K71_001349"
     CDS             13452..13613
                     /locus_tag="U2K71_001349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEM7315441.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERIKAVMVNETARKAINLHIVNL
                     RREIRRLEE"
     gene            13616..13915
                     /locus_tag="U2K71_001350"
     CDS             13616..13915
                     /locus_tag="U2K71_001350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315442.1"
                     /translation="MGKKAAMKRIIELTHSENWQEDKEIVAEVQKLGKSMWTEKPKRK
                     TPRKIAIWHGDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEEK"
     gene            13915..14229
                     /locus_tag="U2K71_001351"
     CDS             13915..14229
                     /locus_tag="U2K71_001351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEM7315443.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKAKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQTNWSKKLHQDRFKFVEKYREILNEYFE
                     ED"
     gene            14406..14717
                     /locus_tag="U2K71_001352"
     CDS             14406..14717
                     /locus_tag="U2K71_001352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HEM7315444.1"
                     /translation="MDELITKVEQWAKDKGLDQADSSKQMLKTIEEIGEVAASLARKD
                     EHGLRDGIGDVVVTLIILAMQNDMDLYECLNQAYNEIKGRTGKMVDGVFVKSSDLEDS
                     K"
     gene            14714..14932
                     /locus_tag="U2K71_001353"
     CDS             14714..14932
                     /locus_tag="U2K71_001353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphocarrier protein HPr domain protein"
                     /protein_id="HEM7315445.1"
                     /translation="MISLTIFWCVIGGLLAMLGAYLIYESAKGKKYTAVGIILFVVGI
                     LVAIGAEINLYLNGTTEELMWFLLWTRR"
     gene            14945..15226
                     /locus_tag="U2K71_001354"
     CDS             14945..15226
                     /locus_tag="U2K71_001354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315446.1"
                     /translation="MGDMGEYWKDIKPHLKERRKQHVRKMGNSATKNIETLGFEFTHY
                     PNNHQFAINTHNGIIDYWGTTGTWIERKTKKRGKGIRSLRKYINQEDSK"
     gene            15223..15549
                     /locus_tag="U2K71_001355"
     CDS             15223..15549
                     /locus_tag="U2K71_001355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315447.1"
                     /translation="MNYTQQELTDLCPKHVAEFINNEVLPKYADGLNTAENVTDFMIN
                     DAIDRLIFLEIDCIAYYRLHAEVALIDPYIALSQNRKILVAYIDTKQILEGRDVEIEI
                     DEEEVG"
     gene            15546..15743
                     /locus_tag="U2K71_001356"
     CDS             15546..15743
                     /locus_tag="U2K71_001356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315448.1"
                     /translation="MKAYTIKFYGNKTYVYILSGLRLDFRGYSKEDLLESLYGYGYLN
                     EDELISLEKFTNAWTIEEEAE"
     gene            15740..15949
                     /locus_tag="U2K71_001357"
     CDS             15740..15949
                     /locus_tag="U2K71_001357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315449.1"
                     /translation="MKIYVVKFGNKFYRSDERSIGANTLFIVDILQSARWFDSLEEAN
                     QVSRRLGGLTQVYELVTVDHEEVER"
     gene            15946..16233
                     /locus_tag="U2K71_001358"
     CDS             15946..16233
                     /locus_tag="U2K71_001358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012197617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315450.1"
                     /translation="MKLKDGFYASSHGIGGLMLDMPTKNPKKRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVARLVDMKAI"
     gene            16825..17290
                     /locus_tag="U2K71_001359"
                     /pseudo
     CDS             16825..17290
                     /locus_tag="U2K71_001359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
     gene            17589..17930
                     /locus_tag="U2K71_001360"
     CDS             17589..17930
                     /locus_tag="U2K71_001360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315451.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDPRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(17923..18114)
                     /locus_tag="U2K71_001361"
     CDS             complement(17923..18114)
                     /locus_tag="U2K71_001361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEM7315452.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSDNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDVTK"
     gene            18464..18676
                     /locus_tag="U2K71_001362"
     CDS             18464..18676
                     /locus_tag="U2K71_001362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315453.1"
                     /translation="MNENQLRELFKTNEANKTMEATFYETQKSLALIAKQAKYFYDQL
                     ILQGFNDAQAMEFMMRTFSASNQQKE"
     gene            18683..18907
                     /locus_tag="U2K71_001363"
     CDS             18683..18907
                     /locus_tag="U2K71_001363"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315454.1"
                     /translation="MRNYWYVSLSNKYPQPNADDPIRVVQSVQIKKKYSIIEMTREAT
                     PKEIDKYNLRYCGHGYFSEQNIQTNIERYC"
     gene            18934..19467
                     /locus_tag="U2K71_001364"
     CDS             18934..19467
                     /locus_tag="U2K71_001364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase small subunit"
                     /protein_id="HEM7315455.1"
                     /translation="MVNLTPKQKAFADEYIKNGGNATQAAIKAGYSKRSARVIGKENL
                     TKPNIIQYINERLNPIEKKRKLSAEDALNELIDIWQGEVQISVSKQIDRLEKNKVIKH
                     MQYEYTPDLESKLKALDLYLKYKSLLSQTQLEKAQTEIKLMQAKLEQLQINSERSTEE
                     KLDELLEKISGELDGTS"
     gene            19454..20698
                     /locus_tag="U2K71_001365"
     CDS             19454..20698
                     /locus_tag="U2K71_001365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBSX family phage terminase large subunit"
                     /protein_id="HEM7315456.1"
                     /translation="MALVDIYNPKQIDVLNKTIKNDWFITLLHGAKRSGKTKINNDLF
                     LFELRRVRKIADEEDIKEPMYILAGVSSATIQKNILQELYNMYSIEPKFDKHGNFKLF
                     GVKVVQAYTGNIGGVGAIRGMTAYGAYINEASLARQEVFAEIVSRCSGTGARILADTN
                     PDNPEHWLKKEYIDNSSKNIQSFHFELDDNTFLSERYRNNIKESTPSGMFYDRDIKGL
                     WVSAEGVVYRDFDASKHYIQSKDLPPLSNFYCGVDWGYEHWGSIVVIGETDDGTAYLI
                     EEHATQFEEIDYWLDVAKGIQERYGLRVPFYCDSARPEHVARFVREKINAKNAHKARL
                     SGVEEVAKRFKQDKLFICQDRVMKFRDEIYQYIWDKKKGEPIKEYDDVLDSVRYAIYT
                     HELLKKPKVNVNEKIKRIKRMF"
     gene            20711..22210
                     /locus_tag="U2K71_001366"
     CDS             20711..22210
                     /locus_tag="U2K71_001366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM7315457.1"
                     /translation="MDKVNEFEYGADIHYSNDVNTNYVKFSVDSNLHYRFSSAEDLLN
                     DLDTLAAMIKHHHEYQVKRLSVLDDYYKARNTNIMDNRRRREKEKADHRSAHNFGKVL
                     CTFDVGYNTGNPIKVQIEDTNQQKEIEEFNTNNDIDGLNAELWLDMDKYGRAYEIIYR
                     DSDDTDYVDLANVFETFVVYDTTVKREPILAVRYPKTRFNKDADKQYIQPIVYTKEKS
                     ITYDETTLTAIELKNPQDEPHEYKEVPITEYSPNRFRMGLYEDVLSLIDLYDAGQSDT
                     ANYMTDLNDALLVISGDIEAAGLSTEDAIKQKEANMLLLESGTDVNGNKTSVTAGYIY
                     KQYDVNGVEAYKYRVRKDIHEISMVPDLTDDNFSGVQSGEAMKYKLFGFEQMTATKQR
                     LFKKGLMRRYRLLFSLKSSISEMDNSDLKGLRVIFTPNLPKAILEELKSLVDAGAELS
                     QETILGLASFVPDVQAELKRVNKETQKQIGIFDSDGEEVINNKKDETGG"
     gene            22215..23141
                     /locus_tag="U2K71_001367"
     CDS             22215..23141
                     /locus_tag="U2K71_001367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEM7315458.1"
                     /translation="MNSQEYWIKREKEWQKQQIKDDKKRMAEIKSRMQYAQDAIQKEI
                     DAQWDSFSNGQKITRSEAMKRASEMDVKAFARKAKKYVKEKDFSPTANQELKLYNLTM
                     RVNRLELLKANIGLELISLFNELDKYFSNELTKAGLAELKRQAGILEMTIASSGYAKL
                     IELVINSSFLSDDVSFSDRLWMYQSELKSELDRLLVRSITMGKNPKQLASKLAEYLTA
                     EGRENTKFNTQRLMVTETTRVQVGIQERSYRDAGITQYIYIAEPTACKLCIPLNNQVF
                     DVADMQPGSNAPNMHPFCRCSTAPYIERISSR"
     gene            23268..23870
                     /locus_tag="U2K71_001368"
     CDS             23268..23870
                     /locus_tag="U2K71_001368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEM7315459.1"
                     /translation="MKKRLFMPMNLQFFSEPGDGGSGDEGQQGNLPAGSQETPPEAKE
                     ENNTGKTFSRDEVAKMIAAETNKAKAAWEKELAAKKEEAKKLAKMNAEEKLQHELEQK
                     EAEIAELKRGQALSEMTKEASKMLTDANLPHDDDLLGLIVSDDADATKQAVAVITNFA
                     SLIKRENARQTPPNEGGQFTASKNTKETVAKLAAKNRIIK"
     gene            23883..24806
                     /locus_tag="U2K71_001369"
     CDS             23883..24806
                     /locus_tag="U2K71_001369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEM7315460.1"
                     /translation="MKKKQLLPMNLQMFAQTWDPDNVLVYETKEGKIPDKYNTLILSE
                     VMENSKIMQLAKYEEMTDKEKKFEYFAEGPGAYWVGEGEKIKTSKPKWMQATMTAKKL
                     GVILPVSREYLNYKLSDFFEEMQPKIAEAFYKKFDAAALLNKENPFPQSLDGSVISAG
                     NVVEGGLTYDNILALEDKLAENEFEPNAFISNRKNRTELRSAAQTVGSNVEFIYDRST
                     NTIDGLPVVDLKSLDKGTLYAGDFNYMFYGIPYNISFKISEEAQLSTLTNEDGTPVNL
                     FEQELIALRATMDVGFMIVKDEAFGKISPKA"
     gene            24824..25087
                     /locus_tag="U2K71_001370"
     CDS             24824..25087
                     /locus_tag="U2K71_001370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315461.1"
                     /translation="MPNQKTWTGKVGDTKTFTISAVPADASDAAAVVAATTATSSDGA
                     IATVTKNENGGFDGTIAAEGSATFTFTSGEFTTSINVTGQPAS"
     gene            25099..25428
                     /locus_tag="U2K71_001371"
     CDS             25099..25428
                     /locus_tag="U2K71_001371"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head-tail connector protein"
                     /protein_id="HEM7315462.1"
                     /translation="MMIADDIKKLLKGTIDEKLEVIERRTNERMKTLLNTQEVPKEFE
                     TVVYEVSLKRFNRIGQEGMQSYSQEGLSMAFPDSDFSEYQNEIDEFKRKDQEELYKPK
                     RGRFKFV"
     gene            25425..25751
                     /locus_tag="U2K71_001372"
     CDS             25425..25751
                     /locus_tag="U2K71_001372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315463.1"
                     /translation="MRFTDEIIFVKRSSDSKYDPDLGEWVEGKPERTRTEANVTDIGT
                     DRSVTIFGSVEEGAKVIRTQPLFSIPTFDYIEIEGKTWQQKTARNPAYRNSLIVQEVV
                     LDEGTT"
     gene            25735..26073
                     /locus_tag="U2K71_001373"
     CDS             25735..26073
                     /locus_tag="U2K71_001373"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEM7315464.1"
                     /translation="MKAQLEYKGIDQLMRHLKKAATLNDVQKVVKSNTAEMTERMQKG
                     APVDTGYLRRSINMNLLEAGLTGIVGPTADYAPYVEYGTRFMSAQPYVRPAFNYQKVK
                     FMAEMKALVK"
     gene            26073..26465
                     /locus_tag="U2K71_001374"
     CDS             26073..26465
                     /locus_tag="U2K71_001374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage capsid protein"
                     /protein_id="HEM7315465.1"
                     /translation="MIKTRDQSIFDELFKISQNKLGYKTYDYKTLEDVGYPFVEFENT
                     QTIHEVNKTDIKGSVIVVLSVWGLQKKRKQLSDMASALFNEARLIEATEGYYWALNYQ
                     ASGIQVMDDTTTNTPLKRAVVTLEFRIR"
     gene            26476..26988
                     /locus_tag="U2K71_001375"
     CDS             26476..26988
                     /locus_tag="U2K71_001375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major tail protein, TP901-1 family"
                     /protein_id="HEM7315466.1"
                     /translation="MEALKGIDVILLYRLLKKETQEAAWKMAFQTEHENGLSRDSDST
                     VTKDGNVQSLSPVEYDFSATSIVAKGDSHVDEMKQALLNGDIIEIWEINKAEQGTDDN
                     ANKYKATYYQAYVSEFTPSAAAEDNVELSLSFAVNGVGQDGYATLTEDQADVVQYAFK
                     DTVKATSTGA"
     gene            27034..27402
                     /locus_tag="U2K71_001376"
     CDS             27034..27402
                     /locus_tag="U2K71_001376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tail assembly chaperone"
                     /protein_id="HEM7315467.1"
                     /translation="MKLKIKGKEYSFKFGTKFVRELDKVMPFVDGNMEFGMGLSAKVL
                     PELRSYNVNTLSRVLEIANRTEDESITLDELDDYIDEVKDIEKLFDEVLKELAESNAG
                     KLAVRNLNQKLKEAEKQQVE"
     gene            27456..27743
                     /locus_tag="U2K71_001377"
     CDS             27456..27743
                     /locus_tag="U2K71_001377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315468.1"
                     /translation="MTNISDIERMTLYEYNIRMTAAQLSWLDKEKLIHELAWANQQVQ
                     AEKKVGKKTVPVYRSFEEFFNYQKIEDSIMGVSELSKQDKKFQSLLTKANS"
     gene            27760..30879
                     /locus_tag="U2K71_001378"
     CDS             27760..30879
                     /locus_tag="U2K71_001378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEM7315469.1"
                     /translation="MEQFSVEALLKATDSGFVKTFKDAQDAVKTFEKNSNSMTTAVGK
                     VMQGTGAAMTKYITTPLIGVGVAAAKVGGDFEAQMSRVKAISGATGDTFEQMKQQAID
                     LGAKTAFSAKESAAGMENLASAGFSAQEIMKAMPGLLDLAAVSGGDVALASENTATAL
                     RGFGLEASEAGHVADVFARAAADTNAEVGDMGEALKYVAPVANSMGISLEETAAAIGI
                     MSDAGIKGSQAGTTLRGALSRLARPTKAMQDTMDNLGVSFYDADGKMKPLKTQVELLK
                     KAFEGLTPEQQQNALVTLYGQESLSGMMALIDKGPDSLGKLTKSLKDSDGAADDMART
                     MQDNMNSSIEQMFGAFESAAIVIQKILAPSIKKVADAISGLVEKFVSAPESTQKLVVA
                     IGAIVAAIGPLIFMIGSVIIWINRVKVAFKALSESSKLFSGLSKAMGLLTNPVFLVIA
                     AVALLVVGFIYLWNTSEDFRNFWIGLWEGIKSAVSSAVEWIQNAWKSTGEWFNNLWKS
                     IKEGADNVWTTVQEAPGKAADWIKNKWTGTKEFFSNLWSSIANSASEIWNSLKEGVIS
                     VIDDLVSSAGEKWEGFKNTISTAWKTITSKIKSGFDFILKYIGPFVSSFSDVFSNIVK
                     AITSIFAEVKNIIVNAWEIIKSLIAAPLLFIIDLITGDFEQMKEDLDLIWNTLVQSVV
                     NIWTSVKNIFTEYIGAIVNSAVSLWTGFIQSISNIWNEVVYQATMIWIDLKLFFTNLW
                     IDIKYSAIQMWINLKFSIIQTWIDTKYGAIELWNNLKQWFFQTVNNIVQTLIKSWNSL
                     KQGTIDLFNNTVQGAKDIWTSFKSWIGDLITGTKDNIIQGWENLKQGTIDTFNNLVNG
                     AQEAWDNLVNAVSDTVDRVTGWFDNLKNIDLLAAGKAIMDSFLEGLQNAWKSVQDFVG
                     GIGDWIREHKGPIQYDRKLLIPAGQAIMNGLNKGLTGGFNDVQNTVGSMADFIAELFN
                     ANSDVDIAANLKNANKNIGAQVEHKINMGGSTKPAVFKINLGRQSFRLFMDDISQAMG
                     EGADINLEF"
     gene            30912..31679
                     /locus_tag="U2K71_001379"
     CDS             30912..31679
                     /locus_tag="U2K71_001379"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail family protein"
                     /protein_id="HEM7315470.1"
                     /translation="MYSFKDTTINLTSSKRFLPTSAMMYDGMYLEDLIDGYQTLTVEG
                     REMLSVEVEQQEIQIGSIITNQKIPSRTLKITYKLEDRDPEKLQFKFKELLNYLYRNE
                     DVEIRFHDELDYYYYGRYTSTDTVPGDSNSIISSFNVFCADPLKYTKECVSDGYIGNP
                     IQFPITPRKIEVTLSMNNSIKITNGEQNITITDAAIKTGDVLVFDFSDEQVTVNGEDC
                     TSMIDLESDFENFYLKQGQKITSNNGKLKIFYRGATI"
     gene            31676..34507
                     /locus_tag="U2K71_001380"
     CDS             31676..34507
                     /locus_tag="U2K71_001380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEM7315471.1"
                     /translation="MSETVYFFDHLQKLIKRKNTRSLIEVSQEKEISSDKSDLMKDTL
                     YVTTKYDKEIEDARYMAIRENESSFSLYRITKVSDPFETLEFTGLGFATNELDAYIIK
                     DIRPSGQPLKNVLDRLIEFTEGNWRVGHVEAMLPTVTATFYYVSVKEALKELQTLGME
                     FVFRCSLNSDGIKDKWIEVYEQIGEESNTRFVYGSKALTVVREIDRSSISTSMIGRGR
                     GEEVGDGYGRRIEFTDVEWKKSNGDPLDKPKGQNWLEDPESTQKYGIPQKDGSMRKRE
                     TVVVFDDIDDPTDLLKNTYSTLIDSARPLVQFKAEVTGGDVIGNTVTIHRYDKGYHYK
                     TRIYKTTFNRLTGQTNIELGDNLTQDVRKQTASIVNNINSLESSKMTFYESTEIGKYQ
                     DDIMRGAGDNGGSIYWVNGIEAGVSDSREIYETVYMDGPNIPRSRFFMVQNNSGISFK
                     QCKKGEWQTIQDVHNGDSTTAWTLDGTFNANFIRAGILSGILVQGVALKTFDDKDFQL
                     VAEGGELSFEKQVKSTGLDDVHGERLGSIVSTYGSKGINGFAVWKEPNYIFSINAGDG
                     GDRGNPIFQIPADVTADKRKYNLYGDGNFSEGNITIDGRLDVKELYVNGTKIDTNGGG
                     NSGGNDNGWNGQYPPEVTTDRDKRYWQIWATAIGAGFTQQAAAALLGNAQGESDANPT
                     ADEGNGAPGFGYGVWQWTDSSGATSGRVYMINLMTKAGISDDPDTITAQFKLLMWHAP
                     NGQWIATSAYPYTWTQFMNLTDINTAAQAFVANFERPRDPHPERTAWSQEWYDKFKDL
                     EIPVSKGYMKPIADPITVTSEFGWRTSPITGAQEFHNGIDLVNGNPNTPIFASADGEV
                     IVAGDANYFDWYGNWTVIKHADGMYTGYAHQSRVDVSKGQKVTAGQQIGLMGTTGPST
                     GEHLHFQFMDEFYPSSSGHFHNARDYIDF"
     gene            34482..36239
                     /locus_tag="U2K71_001381"
     CDS             34482..36239
                     /locus_tag="U2K71_001381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEM7315472.1"
                     /translation="MQETTSISKGGIVMAETQHKMVLSTTEPNNGINLVRIRQGDVLT
                     QKFVVEVVEHGKLKTFEGLVPFFINTTKFGENQPVEQKVQEYSPAQARLVYTLSEPDW
                     QWGGENTAHFSFRSLNGDGTWSEQFSTQDFTYRVISGISRSQLRDSGYVWTFEDLLRK
                     FKDYMDQGKNDWEQWLEDNREILENIDPGGMIINILNEAKGDYESLADRLNQKYQVPV
                     GSSQIRETTRFFDYDTMKYVDLVPRNLNTVVNSVNNSKFNFSFITDIHADNHNLRIDG
                     VGYKDASYLRHWRAIPQFQKLGNKTDVMIYGGDNIDGGLGSLGSDIGIIDEWSARHSM
                     LGTLKRFTNAAVAGQEKPVIICKGNHDACFDPAWRKRKGMLCNADFEEYWNGLYGGVL
                     FPDKNAAIYRFDTCDFYEGGTGDKYVDGYSDTAPGAFSAKQINAFGEWLVNVPRNYHV
                     ALVGHTPLGLSKFPVRNENMISTLIEGFKSGSPVTIDWSKLGQPNDGSFGGLKTFAMN
                     TKGAGVVVGYFCGHWHEQVEGAFGTVKMILCDVGFSHTASQVDTPDELAFYKIEVDTA
                     TRKVTSKGVGRARDFTYNY"
     gene            36256..37038
                     /locus_tag="U2K71_001382"
     CDS             36256..37038
                     /locus_tag="U2K71_001382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315473.1"
                     /translation="MLDFQSKPNIFEEMSYEEAVKWLLRQAAIHYDGSDHDAHVLATE
                     SNAGFATPETVMQARGRWLRDYKLPQKYPNILDIPPGKYATKAGWGADNPDGIEDDSF
                     VEMMVFADHDSRKLIVAFARYSGEIYIKMTHNSEPVEGYNSLGWRRVYTTSVLFEGEL
                     RKGQSVNLPDDTFRYQTLRIHYTDGDGDFVEEVKRQRYARITKANLWNGDAGVTFIEF
                     ELTIEARKITMSNGRALDISSGNVSNPAMSNNVKITKIEGVK"
     gene            37038..>37779
                     /locus_tag="U2K71_001383"
     CDS             37038..>37779
                     /locus_tag="U2K71_001383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BppU family phage baseplate upper protein"
                     /protein_id="HEM7315474.1"
                     /translation="MAHVIKKGSIKVPTQPKDYDLQATGLVFKSYDNQIALEFNVEQQ
                     NGTPADLLGANLRLLMFIYDEVDGMITKEPIPFITKNLITESFLNGQVVYILPEAMKA
                     YNGMVEAYVYIEYPDGSTSDNLGFTFRMKRSAIDGLAQDKADYFIADFQQLLDGVKQE
                     ATDAVNAALTNVDSTIEKANQQINEFVEGATQAIDQTVDEVTEQLQATQTKIDTVSQN
                     VTSAQNNLKAVEDKMNQTNQQIGDLGKLK"
BASE COUNT        13251 a         6059 c         7850 g        10619 t
ORIGIN      
        1 aaataaaaaa agcgttagca actatgctta aaaagcatat cgactgaata aactaagcat
       61 tggacaggct gaaagactac atttaaaatg aaatgcgaag aaacaataga aaataaagtt
      121 gccttagaac catagttgaa tcacctaaca gtcgtgaagg gagaataaga gaaatggcag
      181 aaaaacaaac cgcaggacga gatcaattag gagattttgc accaaaattt gcagaattga
      241 atgatgatgt tttatttgga caagtatggt ccagagaaga attatctgct catcagcgta
      301 gtatcatcac catatctgca ttaatttcag gaggaaattt tgaacaattg ccagctcatc
      361 tgaagattgg caagcaaaat ggcattacta gtgaagaaat agcagaaatc attacccatt
      421 tagcttttta tgtaggatgg ccaaaagctt ggtcagcttt tagtttagca aaagaaatct
      481 atttagaaga aaatagcgaa gataagaaag aggcgtaaat gacgacaaaa cacgaagaaa
      541 tacacaatgg cgtgattttt ccagcaggtg aaaaaaacga agcttacgct caagtattta
      601 ccgggcaaag ctatctgaat atgttagtga atgatcccga agtttctgtt ggtgtaggta
      661 atgtaatttt tgaaccaggt tgtcgcaatc actggcatgt ccataaagac ggctatcaaa
      721 tcttacttgt gaccggagga gaaggctggt atcaggaaga aggaaagcca gcaagacgat
      781 taatggctgg agatgtcgtc gtaacgcaca aagggattaa acattggcat ggtgcgacaa
      841 aagacagttg gtttgaacat ttggcgatca ctgctggtaa ttcagagttt ttagaacctg
      901 taacagatga agtgtatgaa ggattggaaa atcaataatg tcaagcttga acagctcctg
      961 aaagtagatt ttaatcgaac tttcaggagc tgcttttttg attcagtctt ttataaatag
     1021 aaaatagggt taataatagg aatgagaagc acgtcgaaag atcttaatat tgataatcgg
     1081 ctgggcatac atgatttcca cgtcatcaat caaactacct aacagatgat aatcttcccg
     1141 atcatgtcgg ggattaccta atagttcttg atagtagatt tccatactag ggtcccgagg
     1201 attgttcagc aaatcagcaa gtacatctaa atcttctggc tgagtttctg ccgtaccctg
     1261 tatctctatg aaaagccctt cctcagcaaa ttctactcca atacgtattt cagttatttc
     1321 atgggaatat aaataagtaa gaagctcatc gatgattctt tttgataatt ctttttccaa
     1381 gtgtcttttt ttcctttctg acagcggtaa agttatctag tatcggtaca aggatacctg
     1441 caacgaaacc agttgaaaaa ccactgttat atagatttag tcctccgtgc aaagtgctga
     1501 cgttatggac aagagcaata tgtagtacac cagctattac accgtaaaaa ctgccgtaaa
     1561 aaccgcttat gggcgcgaga cctgtcccaa acacagctgc aacaagcatg ccagtactgg
     1621 tgatctcatg aaaagttcca aacaaagaag caataaatat tccgactagt atcggaaaac
     1681 tgttttttaa atgacaacca aaagcactga aacccaccgc agataaaata gcaccgatga
     1741 caggtccatt taactgaccg ccaaccagca aaacataaga agacagcatc agaccaacta
     1801 agcctatgtt gatcaaagta gcaccaatac cagaattagc aatgaaatca gttgtcaatt
     1861 ttccagatga atcgaaaatt tctcgaatcc ccaaaagccg aaaggaatta taatagaaac
     1921 caattccgac tgtcagcgaa aaaaagccaa aaataatcca aatgagagga aagtgatatt
     1981 cattgaaaac caatgtattt tcgacgattg aatagctgaa taacctcaaa acagcagtaa
     2041 aagtcatcgc aatcaaccca gaggtgaatc caatattata aagtgtgaat ccacgatgga
     2101 aaactaaagt ttgagcagct aagggcggaa gaagtagacc aattgctatg ccagctaaat
     2161 tccccaataa aatacctatc agtaaaggaa aacgcatacc aaaagtgata taactaacga
     2221 ccggtgataa tgcgctacca aacagtccga tcatgatgta ctgagaaaaa ggtttattga
     2281 caaatacagc gtatagatac acacctaaaa tgattgagat agaattataa aggtttttgc
     2341 caaaaaaaga gaaaccggta actgttagaa tcgaagcaaa taagggaccg tttaaaatca
     2401 ccttgtgccg ataagctaaa agcacagaaa gtaaggtcaa aatccctaca tttatgaatg
     2461 cgcttccgaa accgcctaaa gcaaaataat ctgtcaaaag attgcttggc gaggtaagaa
     2521 tagttagcaa tccactccaa ttatcagtag gagtattaga aagcaagcca ataaaaagaa
     2581 aaaaaacacc tgtaaaacca agataaaaat actttctttt ctgtgaaacg attcgttttt
     2641 gaggatgaat ataaataacg tcagtttcgt tttccattac acaccttctt tttcattttc
     2701 tttaactata acttgaaatg aaagatttgg acaactatca tttaggtcaa aactaaaaat
     2761 gtaagggaac ccattgaatt taaccaaatt tttttaattt tgcaaaaaaa agttaaagaa
     2821 gactgaaaaa aaatttaaaa tataacgttt tgagtaaaat gtttgcaaaa aatgctaaaa
     2881 atagggtaca ataaaacaac atatactgca gtaaagaagt gggggagttt taatgagaag
     2941 aaaagtctat accaaggatc aaattctaaa agctgcttat gatgttgttg caaaggaagg
     3001 gtttaaagga tttactgcga gaaatattgc taaaaagatg gggatttcga cacagccgat
     3061 ttatctggaa ttcaaaaaca tggaagattt aaaaaatacg ttgctggatt ctatctttga
     3121 acaactaaaa gaagatgttt tccctattga acgtactggt gataaaatca ttgatctcgg
     3181 tttgaactat atctactttg caaaaaagca tcatagtctt tacatggcat tgtacattga
     3241 tgaacatggg gacggaaaga aaatgcatga tttttcatac aattatttcc gtgaaatagt
     3301 taaagaagat gaaagataca atcaattatc ggatgaatat gtcgatgctc tgcatactgg
     3361 tacatgggta gcagtgactg gcttagcgtc attgatgtca tcaaacatca tgcatccaac
     3421 agaagaacaa atcattgcac tgatcaaacg gacaattgac tcgattttag cattagataa
     3481 tcctgaaata atcagatagt gttctatcag tatgaatttg atcggtaagt ccgttggctg
     3541 caaactgtca atggactttt ttgctaaaaa aagcgatttc tgttatttat ctattagaaa
     3601 taggcttgac atttcgtgaa aaaagtttaa aaatagtact tacggaaggt tggcagagtg
     3661 gtaatgcacc ggactcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc
     3721 tgtaccttcc ttatatatca acgattagac acgatttcga tcgtgtcttt tttgttgtat
     3781 atttagccta aaatgttttt tgactcccct tttgactccc cctgagtact gttcttcata
     3841 aatgctacaa aattattgat gacttttttg ttttgagttt cagttacatg agtgtaaata
     3901 tttgaagtag tttgaatgtc tgcatgccct aaacgttcct gaacatcttt taaagaagct
     3961 cctgattcaa aaaggagaga ggcatgagta tgtcgaaaac catgagtagt gattctttta
     4021 gtcaattctg gatgatgctt atatatgaca ttaagccacg aacgtgaaac tgtagggtta
     4081 tagagaagac catcatggtg aaaaattaag ttttcccttt ggattgtaac gatcgttttg
     4141 ttttttgtat aatactttct caataaactg atcaaatcat tgtccagata aatttttctt
     4201 tttccagatt ttgatttagg agtatttacg atcaatcttc cttgataacc tcttgttaca
     4261 gttttattga tattgagtgt tttgctctta aagtcaatat ccgaccaggt cagagcaaaa
     4321 gcttctcctt ttcttatacc tgtaaaagcc aatagggaaa agaataaata tctttcatca
     4381 tcatcgtttc ttattgcttc taaaaatttg attaattctt ctttagtata aaattcgata
     4441 tttttatctt cgatatctag agcttctcca cgtggaatag aaactaattt cataggattg
     4501 ctagttataa tttgtaaaga agcagcgtaa tcaaaaacat tagatgtgta attttttatt
     4561 ttcttaaaaa ttttaggatg gctatcggac caagtgttta ctgcttcttg acagaaaaaa
     4621 acgtctattt tgtcaatgaa cttatctcca aacactttta agatgtgtgt gtcaaaaatt
     4681 tgtttggtgg aggaccaagt gctttcttta actgtctttt tataattttc aaaccataag
     4741 ccgtaaacat attcgaattt cttgctcttt tgagctgtag aagcttgcag tcctttttcc
     4801 tgtatatctg cctccaatct ttttaaagct ctctcagcgg ctaatggcgt actaaaacct
     4861 cgtctcgttg tttttctttt ttttcctgtt agaggatcaa ctcctaagta cagactaaac
     4921 tgatattttt cttcgccttt ttttgttaag tgctttttaa ttcgtttatc aatttctttt
     4981 tttgccatct ttatctttcc tttcgtacgt ttgttcggtt gtacagcgga tttcgagatg
     5041 gtaaaatagg gtacaacaaa tagacctact ttaccgtagc tctttgcaca tttgcgttct
     5101 tggtcgggcg gcaaatgtgc tttttttatt tcactcttag ttcttgtcct ggataaagca
     5161 tgtagttgtt tggatccata ccgtttaatg caaacagttg atctaccgta attccagctc
     5221 gttcagctat ttgtttggtc ccttcaccag gttgcaaagt caaggtttcg gtttcagatg
     5281 aagaaggtgc cgttggttga gcttgattag ctggtacata atcttgtggt actgattgct
     5341 ccgttgaact gggagtgggt gaaacttgtt caacacttga actaggagta gcttctgcat
     5401 agttttctac actttcagca gagtaagtac tactagtttc aggaggcgta ttctgcgcaa
     5461 tagcagcttg ttctacagta ctagaagatg caatagtact ttctgttgta gtcgaagatg
     5521 attgttcgtt gctacttgtt gagctagtag ttgttctaga ttctttagtt tttttccttt
     5581 ttgtagggtt tcaaaactaa tttctcatta tcatcagatt tgttaggctt ttctggagtt
     5641 aaaataatat ttttatcttc tttgctgaca gtatatttgg catcattatc tttgtcttta
     5701 tcatctttcc aagtcataac atcgccttca agtgtatact caaatttgta attcatttgg
     5761 tcgactaagc ttttagcaaa ttcttctccc atagcctccc aatcgttact tgaagttgat
     5821 ttcatagaac ttgtgtcaac actaaaagat acgacgtgat cagaaaatga tacgatcata
     5881 ttaggttcgt catctttggt tgcttcaatc aaccaatcat tagcttttaa atcttctgta
     5941 gttacttttt taccacaagc agtgaaaagt aacagagaag caaaaactaa taaacttgtt
     6001 attacttttt tcatttttta ttcctcattt ctatgatatg atttttattg gggaatctta
     6061 gaaataaggt ttcgagtccg tgttgctgca cgggcttttt ttatttaaat aaatcccaaa
     6121 aactgaatgt agttttcttg tatactttat tgtaagcagc cttttttggg tctttaatcc
     6181 agccgctacc ttttttccca taaccaggaa taactgcttt ttttactttt ctttttgctt
     6241 ttccagtggt tctagcgctt atggattttt ttatacttgg ttttctcatt cctattttca
     6301 tctatatcct ccttacgttg tattataatt cacaagaaac gatcgaattc atctggtaat
     6361 ccaaagcaga cgagcttttc atattttgtc atatgatcaa agccgtctaa agttggatca
     6421 atagtcagat gagttgcgaa ataatttgct tctttttcaa tttttaattc agatactagc
     6481 gtttttttag acaacatagg tgtgttttca ttcggatgca aaacacagtg gcctaattca
     6541 tgactacaag ttatccattt tcccatctca tctaaactag aatttatgtg gatcattttt
     6601 atctgtttga atttactata gtaaccataa atttctccca aatcttcttc taaaactagt
     6661 atcttcaatt ctcgtgcaat tctaaaagga ttgcgtgttt gatatagttt taccagctta
     6721 ttaattttgt tatcaatttg aggtaaatac ataaacaacg tactcctttt aatcgcgata
     6781 ttttttaggt gtgtattttt tcttagcttc tattttagct attcgcaaag cattctctaa
     6841 agaaataatg agagcttcac gtgtcgcttc actcatttca ttatctttct tcgaaaaagc
     6901 cattccatca gcattagata aatcgtcgat taatgactgc aattcttttt ggatatcacg
     6961 ctcgtctttt tctgttaaat catagtatct ttttttctca gtacgtccta gcaaataatc
     7021 tacagatacg tcgaagtagt cagcaatttc ttgtaacttt tcggcagttg gctgttttcc
     7081 tgcgtttaaa ctataaaagt aattctctcc gtacttcaaa tctttagcga cttgttgaag
     7141 gtttttattc ttgtttttcg ctagttcttt tattctttca aatgttgtca ttccaacgtt
     7201 cctttccaac acgaaaaaat aatttacgaa aaagagtaaa aatagcttta caattttacg
     7261 atatagagtt attatttatc cgtaagttaa tttgatagaa aaaagcaaac taaaaacaca
     7321 ccttatagca taaagtttgg cgaccaagtg caaataaaag gttttgttat aggcttattt
     7381 ctctatgtat tcattttact ctatagtgta aaaatgtcaa tggaaattat attttttcta
     7441 tcattttaac ttacaaattt ataaaaaaag gaagtgggtt aatgtcaaac ttggataacg
     7501 gtcgtgcggc gatcaaagag tttatgaaag agaatggaat aactatggat gatttggcta
     7561 ctgcttatgg tcacacgcgt gtacgaatgc aacaaattct tgatggacac tggagtggac
     7621 caaaggcaaa cagaactatt ctagagatca tccgtgatta cagaattcga taggaggcaa
     7681 gtcatgaata ttctaagcga agagtttttg actcgtttaa gaattgcaat tgttgaagtt
     7741 gtaaaggacg cacttagtca actttcaaaa aagaatttgt cagaaacacg atatttaaaa
     7801 aagatcgaag ctagaaaata tgtgggaggt gtaaacgatc aaggttttga gaagttaata
     7861 gctcacggtt taaaagaaat tcgtatagat ggctttttaa gatatgacaa aaaggacatc
     7921 gatgaactga tggctaaata caaaatttaa aggaggtcaa catgggaaag tttaatcgaa
     7981 cattagtatt cagcgcgcca ctaattatct acgctttagg tctatggggt agtagacaag
     8041 cattaatcgg cacgatcgtt tacatgcttt ggattttcat tgggcttgat gaagctgagt
     8101 acagagcgaa aaagccagtc gggagggact gactaaaaaa gtctaatgtc tataagaaag
     8161 gtttgacaat aaatgaaaga attatttttt ttaataactt tcatcatttt ttctatcagt
     8221 gttttctaca ttaacctttg cgttgctaga tttgctttga aaagttttag gaacagcaaa
     8281 cgaaaataga gctataaaag atacgaatgt agtagctacg ttaaattgct ctttagaaaa
     8341 cgtcaatgtc attggaatga taattgatat cgatgagaaa atctttgcga taataactat
     8401 ccatttttca attataaaca tttttaaatt aagcaccatt agacaaaatg tagttcctat
     8461 atagaggata aatgaagata gaaataaaca tattagtcca gttttaaaaa aagagaagta
     8521 atctatgtgg gcaaattctg ttaatatgct tttttgattt aacacaatca aactactgtt
     8581 ggacaaaaat gacagtaagg aaagagttgc tccgattact aaataaaaaa tagttgttaa
     8641 caatattata ttatcaattt ttctgctcat gttttcaccc caccagaatt atatcaaaaa
     8701 ggagagaaga aataatgcaa gaattagtaa ttttgaaaaa taaagaagct gtgactacga
     8761 gcttacaagt tgcagaaagc ttcgataaaa agcacagaca tgttttgacg gctattgatg
     8821 aactaaaaga gggggttgcc gaaaattggg cagacctatt ttgggaagat acttatgttc
     8881 atcctcaaaa taaacaatca taccgaatta tttatatgaa tagagacggc ttctctctat
     8941 tagcaatggg cttcactggt aagaaagctt taagttttaa acttcaatat attgaggcct
     9001 tcaacaagat ggaaaaagaa ttaaaagacc aattgccatc aattccagca actaaacgag
     9061 aaatggcatt acttgctcta gctgcaaacg aagaaacgaa tgaaagagta gacgctatcg
     9121 agtctgattt aaacgatttg aagaacaatc aacttctagc tgaaccagat taccgaacaa
     9181 tttctaatat ggttcgcaac aaaattagag ttatttgtaa tcagcagcat ttaaatagca
     9241 aagcaaaagc agaattgttt aaagacttga atggcggaat taagcgaatc actggagcag
     9301 tagctagaaa tcgtatcaag gcaaaacagt tcgatgatgt tatcgatttt attaacaatt
     9361 ggatgccttc tacagcgacg atgacaatta ttaagcaaat ggagttgtta gaggatgaat
     9421 agagctgaag cgctaaaaat aggcgtaata attgctaatc gctggtggag acacaataaa
     9481 ccaaacatcc taagccaaca acatattgat aagcaaaaag catggcaaca aataaaaaag
     9541 tgactcagcc gaccaaagca atgagtcaca aagaaaatac atctaaggag atgttaccac
     9601 atgaaaaaag aactttccac tctagatcaa tatttgactg atcctagttg gggcaaatcg
     9661 aatatcaagg aaacaagcaa tcgaaaaatc agacgtaatc ttttgacgga tgaagaacta
     9721 gcatgtgatc aagacgattt gggaaatttt gtgagtattt gggatcatgt ctatcttatc
     9781 catttatcga ggaagtccaa aaaaactgaa tatatctatg tcatcgaaga tggcttgatt
     9841 gatgcgctag aagagtatga cagagataac ttgattgata tctcttatta cggatcaggt
     9901 aagaaataca ttgctgaaat ggaggcagaa tttgatgagt gaaaaggaac aacctttgaa
     9961 aaatagaagt gataatacgc tttttaatac cttatacaaa ataaatgtga aagatgttac
    10021 tgaaaaacga aataacctca cttatctttc gtgggcatgg gcttgggcag aagttagcaa
    10081 agtgtgtgag gcagtagatt atgaaatcta tcatgatcca gaaacatatc tgccgtatgt
    10141 ctttgataag aaaacaggct atatggtttt taccagtatc acagtcaacg gagtaaagcg
    10201 tgacatgtgg ttaccagtca tggatggtgc aaacaaggca atgaaagatg agccatatac
    10261 ctacgaagtc aatgattatc agtggaataa cgaaacgaag aaaaaagaga ttgttggaaa
    10321 aatcgaaaag cgagttgaag cagcaactat gtttgatatc aacaaaacga tcatgcgctg
    10381 tcttgtaaaa aatctagcaa tgtttgggct agggctatat atatttgctg gcgaagatat
    10441 gccagaagat gtctcgatgc ttgaaccagc tactcaaaga agcaaaaagc tattcttgga
    10501 tgctttacaa ctggttgcta acaagtacga aaaatcaatt gatgaagcaa ttgttgcatt
    10561 gactgatgcg gcttctataa ccgctgatga cagtaaatgg accaagagag acttgggcat
    10621 tctaaaacga ggcgttaact ggattgaaga tcagtacaga gaagaaacaa aagagaagtg
    10681 atatgagtgt ttaaaccatt gatcgattca tattcagcgg ttctgaaaaa gttcaaagga
    10741 aaagacatag gtgcaacgat caatgaagaa gtgaacattg atcgactaaa gacgatgtat
    10801 gacggatatg atggcgatcg gattattgaa gttcgattta ttgatccacg tcgatttact
    10861 gtgcagcaac gaaacttcat ctatgcgctg ataggcgata ttttcatcga tacaggcatg
    10921 ccaacggact tctggaagga attcttttac ttccgttttg aaggcgtcac agggcgcaaa
    10981 ataagcctca aagacgaatc gaatacaact gtgagtgatg ctaatgtctt agcaaatatc
    11041 atcttagatt tcatctttga acatcatatt cctttcaaag aaggctatga gattttacca
    11101 gcgaatcaag aatattactt ctacaaatgc atcacaaaaa gagtctgctg catctgtggc
    11161 aaaacaggag ctgacatcga tcactttgac aaagcgctag gaagacgaaa gcgcaaagaa
    11221 gttgatcatt cagagtacac atttgcagca ctctgcagga ttcatcacac ggagaaacac
    11281 aatataggtg tgatcaattt caaaaataaa tatcaaatca aagggatcaa gttaaaccag
    11341 gaaacaatta agaaacttag aataggaggg taaaaaatat tgtctgacaa caaacgctac
    11401 tactatttaa aactaaaaga gaattttttc gatagtgacg agatggttct cttagaaagt
    11461 atgccagatg gctatattta ttctaatatt cttctcaaac tttatttaag aagtctaaaa
    11521 cacgaaggta agttgatgtt taatgacagg attccattta actctacaat gctcgcaact
    11581 attacaagac attctgtagg agtcgtagaa aaagcggtac aaattttccg tgatttgcag
    11641 cttattgacg tattagataa cggagcaatt tatatgtctg atatacaaag tttcattgga
    11701 aaatcctcaa ctgaagcaga tagaaaaaga gaatacagaa agaaaataga agaggcaaaa
    11761 cggaatttaa taactggagg acaagtgtcg gacaaatgtc cagacaaaac tacaccagag
    11821 ttagagatag agatagagaa agatatagat atagataaag aagaaaagaa aggtaagtat
    11881 tctgacgaac acttacgcct tgctaaaaaa ttgcaaagta atttaactga agattttcca
    11941 aaagaaatga acaaagtaga tatcgaaaaa tgggcagaca caatcaggtt gatggaagaa
    12001 agagataaag cgtctataga agcgattgag tatgtgatca attggctacc tacaaatgaa
    12061 ttttggtttg gaaatattag aagtgctaag aaattgagag aaaaatttga gaagctcaaa
    12121 ttcgaaatca aagcagacaa gaataatcat aaaaagcaaa gtcaaaaact acagtacagc
    12181 aatcctagtg aatatgacga cttgccaatt taaaaaggag atgcatcaca tggaaagcct
    12241 agcaaatgct atggagaaac taataagaag agtattagtg caaagcggga aatgtccaga
    12301 atgtagcgaa cctttgtata gttggcgagc taaaaataag gatggttcag aacgttgtaa
    12361 accaacatgc atgagttgtg gttataaagc gttacgtgtg aaagaggata tacagaccga
    12421 acggatatat aacgacagct taaaagcacg agcgttgagt ttttttcaaa atggttcggt
    12481 attaacagat aaaactttgt ttaaatgcaa aatggagaat tatcacgtag tggaccaaga
    12541 aacgaaaatt gctttagaaa gagctaaaag ctttgtaaat gatgtcttac tgaaccatcc
    12601 tgcacatttc attctatcag ggaaatcagg aagcggaaaa agccacttgt caatggcgac
    12661 agcttgggaa atacttgagc gctcaaatta tgacaagaaa atacttttta taagctatca
    12721 agagttatta gagcaaataa agttttctta taacaatgct gaactgagaa aagaaattga
    12781 aggatcgctt atagccgata tcaaaacaac tgatttggtg gtttttgacg atattggagc
    12841 tgaattaggt agcggggtat caaatagtag gcagtttaca aacaacacgt taaacacgct
    12901 cttagaagcc agacagaaca aggcaacgat catcacaaca aacttatccg gtcctgaact
    12961 aagagaagcc tacggcgaaa gaattgtttc tagaatattt aagaattcag aaggttatgc
    13021 gctgaaattc caacaaacag cagacaagcg cataaaacca gtgaaaggta gtatcgcatg
    13081 aataaatacc gtaataagaa aactgttcat cgaggtatca agtttgattc tatcgcggaa
    13141 gcagagtatt acgatttagc cttgtggcaa gcagaagcga acggctggaa agtaaaactt
    13201 caggaaagat ttgagctgat gccgaaattt gaactagatg gaaaaaagta tcgcaagatc
    13261 gagtatattc ccgacttcac attttataaa aacggcaaac ttgtcaaagt cgtagatgtc
    13321 aaaggaatgc agacaaaaga ctttaagatc aaggcaaagt tgttctgtca tcaatatcaa
    13381 gtgccgttga tattagctaa aaaatatcgg aatacgttca aggaagagcg tttttaacga
    13441 ggtggtccat catgacaaca gaagaagtga ttcaaatgcg tattcgaagc cttcaacgtg
    13501 agattgacga tctggaacgg ataaaggcag tgatggtcaa tgaaacggcg agaaaggcaa
    13561 tcaatttgca catagtgaat ttaagaaggg aaattcgtag attggaggaa tgagcgtggg
    13621 taagaaagca gcaatgaaac gaatcattga actgacacat tctgagaatt ggcaagaaga
    13681 caaagaaata gttgcagaag tccaaaagct cggtaaatca atgtggactg aaaagcctaa
    13741 acggaaaacg ccgagaaaaa ttgcaatctg gcatggtgat cgaattctag taacgggtac
    13801 agctgaacag ttatctgaaa ttactggact gagcaaaaac atcatctggg atagagctag
    13861 gagcttatgg attgattcaa aggggcgaca gtttaggtat gtggaggaga aataatggat
    13921 ctcattacac aatacagtga tatcatcctc aagaaaatca tgatgaagat tcagaaagat
    13981 aaaaaagcaa aagaacgagc ggaattagtt aagttggaaa tggctgaaac aggagcagga
    14041 gtgcgaagta gcaggcattg gaaagcagca gcaaacattg aattttatta caacgaaatt
    14101 caaaaagggt tcgatcagat gcgtgagctg gatcggcaaa caaattggag caagaaactt
    14161 catcaggatc gtttcaaatt tgtagaaaag tatagagaga tactgaatga gtatttcgag
    14221 gaggactaga tgtttaattc aacgttagcg tcaattcact ttttattcta tataacgata
    14281 tttgtagcta tattaattaa tgcgataaaa agcggttttt taacaccaat cggcacaatt
    14341 ttaacattag cttcatttgt tacaagttgt attatccaac ttaaatatat aaaggagaat
    14401 gaataatgga cgaactaatc acaaaagtag agcagtgggc taaagataag ggattggatc
    14461 aagctgattc cagcaagcaa atgttaaaaa cgatcgaaga gatcggggaa gttgccgctt
    14521 ctctagctag aaaagatgaa catggtttaa gagacggaat tggagatgta gtagtaacct
    14581 tgattatttt agctatgcaa aatgatatgg atttgtacga gtgtctgaac caagcataca
    14641 acgaaatcaa aggacgcaca ggaaaaatgg tagatggtgt attcgtgaag tcgagtgatt
    14701 tggaggacag caaatgatca gcctaacaat attttggtgt gtaattggcg gcttactagc
    14761 gatgttagga gcatacctca tttacgagag tgcaaaaggc aaaaaatata cagcagtagg
    14821 gattattttg tttgttgtcg gaattttagt tgcgattggt gcagaaataa atctttattt
    14881 aaacggtacg acagaagagt tgatgtggtt tttattgtgg acaaggagat aggaggacag
    14941 cgaaatgggc gatatgggcg aatactggaa agatattaaa ccgcatctaa aagaacgtag
    15001 aaaacaacat gtcagaaaga tgggcaattc agcaactaaa aatattgaaa ccttgggatt
    15061 tgaatttact cattatccaa acaatcatca atttgcaatt aatacccata atggaattat
    15121 tgactactgg ggaactacag gaacgtggat tgagcgcaaa acaaaaaaac gtggtaaagg
    15181 gatacgtagt ttgaggaaat atattaatca ggaggacagc aaatgaacta tacacaacaa
    15241 gaactgactg atttatgtcc aaaacatgta gctgagttca tcaataatga agtcctgcct
    15301 aaatatgcag atggtttaaa tacagcagaa aacgttacag attttatgat taatgatgct
    15361 attgatcgtt tgatattttt agaaatagat tgtattgctt attatcgtct acacgcagaa
    15421 gttgctttga ttgatccgta tattgcttta agccaaaatc gaaaaatact cgtagcctat
    15481 attgatacca aacagatttt agaaggcaga gatgtcgaga tagagatcga tgaggaggaa
    15541 gtgggatgaa agcatacaca atcaagtttt atggaaataa aacctatgta tacatcctaa
    15601 gcggtctcag acttgatttt agaggctatt caaaagaaga ccttttagaa tccttatatg
    15661 gttacggata tttaaatgaa gatgaactaa taagcttaga gaaatttaca aatgcttgga
    15721 cgattgagga ggaagcagaa tgaagattta tgtagtaaag ttcgggaata aattttatag
    15781 aagtgatgaa cgctctatag gggctaacac attattcatt gtagacatac tccaaagtgc
    15841 gagatggttt gatagcctcg aagaagctaa ccaagtttca cgacgacttg gtggattaac
    15901 acaagtatat gaactggtca ctgtcgatca tgaggaggta gaaagatgaa attaaaagac
    15961 ggattttacg ctagtagtca tggtatcggc ggtttaatgc tagatatgcc gacaaagaat
    16021 cctaaaaaac gtaagaaacc aaaattcaaa gtcggtgaca tggttcgctg cgaagcagaa
    16081 gggttcatct atccatttcg tggatatgta gaacacgtct ataatcactc agcgatcatt
    16141 cgtattgaaa acacgatgga atgtgataag tggttagcga aaagcaaaga gaatttagct
    16201 gtagcgagat tggtggatat gaaggctata taaacagcga aaaaatgtac ttctaagaga
    16261 aaaatttatt ttttataaaa ataggaaagt atcattatga aaaagaccac agagataaat
    16321 aaacctacca cataaaaaat gaatatttct tgcgattcaa tataggtata tttaacaatt
    16381 tgtacaataa aaaatactat aggtaaaaaa gataacatga ggagcttatt tgttctagag
    16441 tgtttgacaa agtaacaaaa tgcgacgata ccaatacaaa cgggaatcca aaataacagc
    16501 atgctccaca tagtcactca accatctcct atacgcttta ttatcaatag ataatatcat
    16561 ttttcgtaat aaagataaaa aaataagtaa ggcttctcat taaaatataa aaaagacagc
    16621 cgaccactgg ctgtccttag cagaatattg aaatagtgag ccatctgttt tccgccagat
    16681 agctcaacat gtatatgggt aacgagtgca acctcgctac ttgaaaagct tagcataagc
    16741 atgggtataa aaaaagccgg attcctccga ccttaattaa taattctgac acaattatta
    16801 tatcataaat ggaggaatca atggatggta cttttcgatg taaagaagta tgagacacca
    16861 gaggcaaaag atgtggatat ggatcgcaca aaacataatg ttggtgtttt tctttcagca
    16921 tatctgtcag ctagatgtag gataggtcaa cctcgggagc ctaaagtaac ggcatcttac
    16981 tccttggttc caccttctac agcaaataat acatttgaag cagaacgaat gatgattgag
    17041 agaggaagcg caagaagaat ttgagtactt gcacaaatta tttgttcgtg gctattcagc
    17101 aattcagcat ccgcataaac ctgatgtaac agaaaggcgc aagaagatat tctatgatcg
    17161 ttatatcaat ggtctgccca tttatgtaac tgctcaaagg aataatacta gcgaagaatc
    17221 ggttaaagta gaatcaaaca gaattatcat ccaatttgct tcatcgttag aactggttgc
    17281 ttttaagtag ccagttttta cactttttat accctttgat tacccatttg gtttccattt
    17341 tatacctttt ttataccaat cacttacctg gtaaatgatt tattatgata gtgtcgaaag
    17401 attagtgata ggtctgagac aaaataataa taaaaggaac atcgttttat tattgtttca
    17461 caattaagct tcgatagaca gcaacggaaa tattaagaat aaggatgtga attccaactc
    17521 cttctaaatt gttcttatta tctatcatcc gttgctgttt attaatttat gtattggagg
    17581 gaaaacgaat ggaagtcgag caaataaagt atcaaggtat ggatcgggaa cggttgattg
    17641 atattattga gcaacaacgt ttagaattga tccaaaaaga agctgtatgt aaagagtaca
    17701 aaaaacattt agaacaagta attgagtatc attcagtaga aaagtacaga tcagtggtgc
    17761 aaaaaaatag agcaacgagt cctgatcctc gtcaagatgt atcgattagt atcaagacgc
    17821 cagtatttaa ttctgttaaa tcagaaaagc tagaaagtat ctcaactgct ttggtagaaa
    17881 aagcagctga aatgaactcc ctagcaaatg aagttagtcg tcttatttag tcacatcgat
    17941 aacttgagag tctttggtga ttccgttttt tatagaatta atggtttgtt cagcggaata
    18001 tttagtcaag tatgtttcgc tagttgctac tacttcattg ttatcggact taataacgaa
    18061 gtaatattgg ccattagttg cttctcttat aacaaagtac aaatttttca ccacctttta
    18121 atttatttca gcggaccact cgctgataaa taaaattata cacttagtat ttattttcac
    18181 aatattaatt tgtcactgtg gcggaaaggg tagacgctaa gcatgtgtgt taggtcaatg
    18241 cttcggcaac catgcaaggt tcgattcctt gccagtgact ttggcaactg agggttggaa
    18301 atgggcgctc aaagtacacg agcaaggcga ggtcgatagt aatcgatgga atcggtgtag
    18361 gttgcttgat agaactataa ctgcatctcg tttatgaggt gtagttttta catattagat
    18421 cactcatcga gtggtctttt tattttggaa ggggagtaaa aaaatgaacg aaaaccaatt
    18481 aagagagttg tttaaaacga atgaagcaaa caaaacaatg gaggcgacat tctacgaaac
    18541 tcaaaaaagc ttagcgttaa tcgcaaaaca agctaagtat ttctacgatc agcttattct
    18601 gcaaggattt aatgacgcac aggctatgga atttatgatg cgaacctttt ctgccagtaa
    18661 ccaacagaaa gagtgatacg taatgaggaa ctactggtat gtatcattat caaataaata
    18721 tccgcaacca aacgcagatg atccaattag agttgtccag tcagtccaga ttaaaaagaa
    18781 gtactccatc attgaaatga ccagagaagc tacgccaaaa gagattgata agtacaatct
    18841 tcgttactgc ggccatggat attttagtga gcagaacata cagacaaata ttgagagata
    18901 ttgttaataa aattgaaagg tggtggcttg aatgtggtaa atttgacacc aaaacaaaaa
    18961 gcttttgcgg atgaatatat aaaaaatggt ggtaatgcta ctcaagccgc cattaaagca
    19021 ggttatagca aacgatcagc aagagtaata ggtaaggaaa acctaactaa acctaacata
    19081 atacagtata ttaatgaacg gctaaatcct atcgaaaaga agcgcaaatt aagtgccgag
    19141 gacgctttaa atgaattgat agatatttgg caaggagaag tacaaataag cgtgagcaag
    19201 caaatagacc gcttggagaa aaacaaggtt attaaacata tgcaatatga atatacacca
    19261 gatttagaaa gcaaattgaa agccttggat ttgtatttga agtataaatc gctgttatca
    19321 caaacgcaat tagaaaaagc tcaaacagaa ataaaattaa tgcaagcaaa attagaacaa
    19381 ttacagataa actcagagcg ttctaccgaa gaaaaacttg atgagttgtt agaaaagatt
    19441 agtggtgaat tagatggcac tagttgatat ttataaccca aagcaaatcg acgtgttaaa
    19501 taaaaccatt aagaatgatt ggtttattac tttattacat ggagcaaaac gttctggaaa
    19561 aacaaaaata aacaatgatt tgttcttgtt tgaattgcga cgtgtgcgaa agattgccga
    19621 tgaagaagac attaaggaac caatgtatat cctagcagga gtttcaagtg caacaatcca
    19681 aaagaacatc ttacaggaac tatacaacat gtacagcata gaacctaaat tcgataaaca
    19741 tggaaacttt aagctatttg gcgttaaggt cgtacaagct tatacaggaa acattggcgg
    19801 tgttggcgca attcgtggta tgacagcata tggcgcttat atcaatgagg catcgctagc
    19861 tagacaagaa gtatttgctg aaatcgtttc acgttgttca ggaacaggag cgagaatact
    19921 agctgataca aaccctgaca acccggaaca ctggctaaag aaagagtata tcgacaattc
    19981 aagcaaaaat attcaatcgt tccactttga attagatgat aatacatttt tatctgaacg
    20041 atatcgtaac aatattaaag aatcaacacc aagcggcatg ttttatgatc gtgatatcaa
    20101 aggtttatgg gtttctgcag aaggtgtcgt ttatcgtgat tttgatgcta gcaagcacta
    20161 catccagtca aaagacttac cacctttgag caacttttat tgtggtgttg actggggata
    20221 tgaacactgg ggttcaatcg tagttatagg tgaaacggat gacggaacag cttatttaat
    20281 cgaagaacat gctactcaat ttgaagagat tgattattgg ctagatgtgg caaaaggcat
    20341 tcaagagcgt tacggcttac gagtaccttt ttattgtgac tctgcgagac cggagcatgt
    20401 ggctaggttt gtaagagaaa agattaatgc taaaaatgct cataaagcac ggttatctgg
    20461 agttgaggaa gtcgccaaga gatttaaaca agataaattg tttatctgtc aagatagagt
    20521 gatgaaattt cgggatgaaa tttatcaata catttgggac aagaaaaaag gcgagccaat
    20581 aaaagaatat gatgatgtgc tagactccgt tcgatatgcg atatatactc atgagcttct
    20641 taagaaacca aaagttaatg tcaacgaaaa gattaaacgt attaaacgca tgttttaagg
    20701 agtgtgaaaa atggataagg taaacgagtt tgaatacgga gctgatatac attattctaa
    20761 tgacgtgaac acaaattatg taaagtttag cgtagactca aatcttcact atagatttag
    20821 ctcagcagaa gatttactta acgatttaga tactttagca gcaatgataa aacatcatca
    20881 tgaatatcag gtaaaaaggc taagtgtatt agatgattat tataaagcta gaaatacaaa
    20941 tatcatggat aaccgtaggc gcagagaaaa agaaaaagcg gatcaccgat cagcacataa
    21001 ctttggaaaa gttctttgta cgtttgatgt tgggtacaac acaggcaatc ctataaaagt
    21061 gcaaatcgag gacacaaatc aacaaaaaga aatcgaagag tttaatacta ataatgacat
    21121 agatgggtta aatgctgaac tctggcttga tatggataag tatgggagag cctatgagat
    21181 tatctatcga gattcagatg atacagatta tgttgatttg gctaatgtat ttgaaacgtt
    21241 tgttgtatat gatactacag taaaacgaga gcctattttg gctgtacggt atcctaagac
    21301 aagattcaac aaggatgctg ataaacagta cattcaacca atcgtataca caaaagaaaa
    21361 aagtatcact tatgatgaga cgacactaac agcaattgag ttaaagaatc cccaggatga
    21421 accgcatgaa tataaagagg tacctattac agagtattct cctaatcgtt ttcggatggg
    21481 cttgtatgaa gatgtactat ctttgattga tctatacgat gcagggcagt ctgataccgc
    21541 caattatatg actgatctaa acgatgctct tctagttatt agtggtgata ttgaagcagc
    21601 aggactatcc acagaggacg ccatcaagca gaaagaagcg aatatgcttt tgcttgagtc
    21661 tggaactgat gtgaacggta ataaaacaag tgtgactgca ggatatattt acaaacaata
    21721 tgatgtgaac ggtgtagaag catacaaata cagagtacgc aaggatattc acgaaatatc
    21781 catggttcct gatcttactg atgacaattt ttccggagtg caatcgggag aagcaatgaa
    21841 atataaatta tttggatttg aacaaatgac ggcaacaaag caaaggctat tcaaaaaagg
    21901 ccttatgcgg cgttatcgtc ttttatttag cctaaaatca agtatttctg aaatggataa
    21961 ctccgatttg aaaggcttac gtgtaatatt tacgcctaat ctacctaaag ccattctgga
    22021 agagttgaaa tctttggttg atgctggagc tgaactcagt caagagacga tcttaggact
    22081 cgcttctttt gttccagatg tacaggcaga gttgaaacga gtaaataaag aaacgcaaaa
    22141 gcagattggc atttttgatt cagatggtga agaagtaatt aacaacaaaa aagatgaaac
    22201 aggggggtga ttaaatgaac tcccaagaat attggatcaa acgggaaaag gaatggcaaa
    22261 agcaacaaat taaagatgat aaaaagcgca tggcagaaat taaaagtcgc atgcaatacg
    22321 cacaagatgc gatacaaaaa gaaatagacg cgcagtggga cagtttctcc aatggccaga
    22381 aaatcactcg tagcgaagcg atgaagcgtg ctagtgaaat ggatgtcaaa gcattcgctc
    22441 gcaaagcaaa gaagtatgtc aaagagaaag atttttctcc tacagcaaac caagaattaa
    22501 agctatacaa tcttacgatg cgtgtaaata gattagagct cttaaaagct aatatcgggc
    22561 ttgaattgat ttcactgttt aatgaattgg ataagtactt ttcgaatgaa ttaacaaaag
    22621 ctggtttagc tgaattgaag agacaagccg gtattttaga aatgactatt gcttcaagtg
    22681 gatatgcaaa gctgatagaa ctagtaataa acagctcctt tttgagtgat gacgtgtctt
    22741 ttagtgatcg cttatggatg tatcaatctg aattgaaatc agaattagat aggttgttag
    22801 tcagaagtat aacgatgggg aaaaatccca agcaacttgc atctaaattg gcagaatatt
    22861 taacagctga aggacgagaa aacactaagt tcaacactca acgtttgatg gtgactgaaa
    22921 cgactagagt tcaggtaggg attcaagaac gaagttatag agatgcaggc attacccagt
    22981 acatctatat agcagaacca acggcgtgca aactatgtat accgttaaat aatcaagttt
    23041 ttgatgttgc cgatatgcag ccaggaagta acgctcctaa tatgcatcca ttttgtcgat
    23101 gcagtacagc accttatata gaacgaatat caagccgtta acacaaatta acggcttttt
    23161 attgtgcctt cttacagctt acaggcgtta aagagaaagc tattttcggc tgaccggcgt
    23221 aactggtcaa atttatcggg tagcggcgta accgtggagg attaatcatg aaaaaacgtt
    23281 tatttatgcc aatgaactta caattttttt ctgaaccagg agatggtgga tctggtgatg
    23341 agggacaaca aggaaaccta ccagctggct cacaagagac accgcccgaa gcaaaagaag
    23401 aaaacaatac tggcaaaaca ttttctcgtg atgaagtagc gaaaatgatc gctgctgaga
    23461 cgaataaagc aaaagcagcg tgggaaaaag aactagcagc aaaaaaagaa gaagctaaaa
    23521 agctggcaaa aatgaatgcg gaagaaaaac tacagcatga gttggagcaa aaagaagctg
    23581 aaatcgctga attaaagcgt ggacaggcac tatctgaaat gacgaaagaa gcttctaaaa
    23641 tgctgacaga tgcaaattta ccacacgatg atgatttact tggtctgatt gtttctgatg
    23701 atgcagatgc cacaaaacaa gctgtagcag tcatcactaa ctttgcttct ttgattaaga
    23761 gagaaaacgc aagacaaaca ccaccaaatg aaggtggaca atttacagca tcgaaaaata
    23821 ctaaagaaac agtggctaaa ctagctgcta aaaatcgaat tatcaaatag gaggaaaact
    23881 taatgaaaaa gaaacaactt ttaccaatga acttgcaaat gtttgctcaa acatgggatc
    23941 cagataatgt cttggtatat gaaacgaaag agggaaaaat tcctgataaa tataatacgc
    24001 tcattttgag tgaagttatg gaaaattcta agatcatgca gttagcaaaa tacgaagaaa
    24061 tgactgacaa agaaaagaaa tttgaatact ttgcagaagg accaggcgca tactgggtgg
    24121 gtgaaggtga aaaaattaaa acgtctaaac ctaaatggat gcaagctacg atgactgcaa
    24181 aaaaactcgg tgtcattctt ccggtttctc gtgaatattt aaattataaa ttatcagatt
    24241 tctttgagga gatgcagcca aaaattgctg aagctttcta taaaaaattt gatgcagctg
    24301 ccttattaaa taaagaaaac ccatttcctc agtcactaga cggatcagtt attagtgcgg
    24361 ggaatgtggt tgaaggcgga ttgacttatg ataatatcct agccttagaa gacaagttag
    24421 cagaaaatga attcgaacct aatgcgttta tttcaaaccg aaaaaatcgt acagaattac
    24481 gttctgcagc tcaaacagtc gggtcaaatg ttgagtttat ttatgatcgc tctactaata
    24541 caattgacgg attaccagta gtagacctta agtctttaga taaaggaaca ctttacgctg
    24601 gagactttaa ttacatgttt tatgggatcc catataatat ttcatttaag atttctgaag
    24661 aagcccaatt gtctacttta actaatgaag atggaacccc agttaacttg tttgagcaag
    24721 aactgattgc tttgcgtgca acaatggatg ttggatttat gattgtaaaa gatgaagcat
    24781 ttgggaagat ttccccaaaa gcgtaacgcc tgctaccggt attgtgccaa atcaaaagac
    24841 atggaccggt aaagtaggcg atactaaaac atttactatt tcagctgtgc ctgcagatgc
    24901 tagcgatgca gctgctgttg ttgcagctac tacagcaact tcaagtgatg gagctatcgc
    24961 aacagtgacc aaaaatgaaa atggtggttt tgatggaacg attgcagcag aagggtcagc
    25021 aacattcaca tttacttctg gagaattcac tacttcaatc aatgtgacag gtcaacctgc
    25081 tagttaggaa gtaaaaatat gatgattgca gatgatatta aaaaacttct taaaggaaca
    25141 atagatgaaa agcttgaagt tattgagcga agaacgaatg agcgtatgaa aaccttgtta
    25201 aatacgcaag aagttcctaa agaatttgaa acagttgtat atgaagtatc gttgaaaaga
    25261 ttcaatagaa ttggtcaaga aggtatgcag tcatattctc aagaaggttt atctatggct
    25321 tttcctgact cggatttttc agagtatcaa aatgagattg atgaatttaa gcgtaaagat
    25381 caggaagagt tgtacaagcc aaagcgaggg aggtttaaat ttgtatgaga tttacagatg
    25441 aaattatatt tgttaaacgt tcatctgact ctaaatatga tccagatctc ggtgagtggg
    25501 ttgaaggcaa accagaaaga acaagaacag aggcaaacgt gacagatatt ggcactgata
    25561 gaagtgtgac tatttttggt agtgtggaag aaggggcgaa ggtcattagg acgcagcctc
    25621 ttttttctat ccctacattt gactatatcg agattgaagg aaagacttgg caacaaaaaa
    25681 cagctagaaa tccagcatat agaaatagtt taattgtgca agaggtggtt cttgatgaag
    25741 gcacaacttg aatataaagg aatcgatcag ctgatgcgac atctgaaaaa agcagcaacg
    25801 cttaatgacg ttcaaaaagt cgtgaaaagt aatactgctg aaatgactga acgaatgcaa
    25861 aaaggtgcgc cagtggatac aggttactta cgaagatcaa taaacatgaa tcttttagaa
    25921 gctggtttaa ctggtattgt aggaccgaca gcagattatg caccttatgt agaatatggc
    25981 actcgattta tgtcggcaca gccttatgtt agaccagcgt ttaattacca aaaagtcaaa
    26041 tttatggctg aaatgaaagc cttggtgaaa tgatgattaa gacaagagat caatcaattt
    26101 ttgatgaact ttttaaaata tcccaaaaca aacttggata taaaacatac gattacaaaa
    26161 ctttagagga tgttggttat ccttttgtgg aatttgagaa cactcagaca atccatgaag
    26221 taaataaaac tgacattaaa gggtctgtga ttgtggtttt atccgtttgg ggattacaga
    26281 agaaacgaaa gcagttgtca gatatggcat ctgctctttt taatgaagct agattgatag
    26341 aagccacaga aggctattat tgggctttaa attatcaagc aagtggaatt caagtgatgg
    26401 acgacacaac aacgaataca ccgctaaaac gggcggttgt cacacttgaa tttagaatta
    26461 gataggagga agaacatgga agcattaaaa ggtattgatg tcattttgct ttatcgctta
    26521 ttgaaaaaag aaactcagga agccgcttgg aaaatggcat ttcaaacaga acacgaaaat
    26581 ggtttatcaa gagattcaga ctctacagtg acaaaagatg gaaacgttca aagtttaagc
    26641 ccggttgaat atgatttttc ggctacttca atagttgcta aaggagattc tcatgtagat
    26701 gaaatgaaac aagctttatt aaatggggat atcattgaaa tttgggaaat taataaagca
    26761 gaacagggaa cagatgataa tgcaaataag tacaaagcta cttattacca agcatatgtg
    26821 tctgaattta ctccatcggc tgctgcagag gataacgttg aattaagttt atcatttgca
    26881 gtaaatggtg ttggtcaaga tggttatgca accttgacag aagatcaagc cgatgttgtt
    26941 caatatgcat tcaaagatac cgtgaaagca acttcgacag gagcataaga gggcttagat
    27001 gctctctttt ttattttagg aggatgaaaa acattgaaat taaaaattaa aggtaaagaa
    27061 tattcgttta aatttggcac taaatttgta cgtgaattag ataaagtgat gcctttcgtc
    27121 gatggaaata tggaatttgg aatgggactc tccgcaaaag tcttaccaga gctacgttct
    27181 tataacgtta acacgttatc acgagttctg gaaatagcaa atagaactga agatgaatct
    27241 attacattag atgaattgga tgattacatc gatgaagtta aagacattga aaaattgttt
    27301 gatgaagtcc taaaagaatt ggcggagtcg aacgcgggaa agttagcggt ccgaaacctg
    27361 aatcagaaat tgaaagaagc ggaaaaacaa caagtggaat agattctgca ctggcatacg
    27421 aacaaattct tatcaattct tttcgatatt tgggaatgac caatatctca gatatcgaaa
    27481 gaatgacgtt atatgaatac aacattcgta tgactgcagc ccagttatct tggcttgaca
    27541 aagaaaagtt gattcacgaa ttagcgtggg caaatcagca agtccaagcg gagaaaaaag
    27601 taggcaaaaa gacagttcct gtatatcgat cctttgaaga attcttcaat tatcaaaaaa
    27661 tcgaagattc aatcatggga gtttccgaac tttcaaaaca agataaaaaa ttccaaagct
    27721 tactaactaa agctaactct tgaggaaagg aggagaatca tggaacaatt ttctgttgaa
    27781 gccttattaa aagccacaga tagtggattt gtaaagactt ttaaagatgc acaagatgct
    27841 gttaaaactt ttgaaaagaa ttcaaatagt atgacaaccg ctgttggtaa agtgatgcaa
    27901 ggtactggtg ccgcaatgac aaagtatatt accactcctc ttataggagt aggcgtagca
    27961 gctgctaaag ttggtggcga cttcgaagca caaatgagtc gtgtaaaagc tatatcagga
    28021 gcaaccggcg acacattcga acagatgaaa cagcaagcga ttgatctagg agcaaaaact
    28081 gcttttagcg caaaagaatc agctgctgga atggaaaact tagcttctgc tggatttagc
    28141 gcacaagaaa tcatgaaagc aatgccgggt cttttagact tagcagctgt atctggaggg
    28201 gatgtggctc tagcttctga aaatactgct actgctttga gaggatttgg tttagaagca
    28261 agtgaagcag gacatgtcgc tgatgtattt gctcgtgctg ctgcggatac caatgctgaa
    28321 gttggagaca tgggagaagc attgaagtat gttgctcctg tagctaattc aatggggatt
    28381 tctttggaag aaactgcagc agctattggt attatgagtg acgcaggcat taagggttct
    28441 caagcaggta caacgttgcg aggagcattg tctaggttag caaggccaac aaaggctatg
    28501 caagatacaa tggataattt aggtgtttcg ttttatgatg ctgacggtaa aatgaaacct
    28561 ttaaaaactc aagtagaatt acttaaaaaa gcttttgaag gcctgacgcc tgaacaacaa
    28621 caaaatgctt tagtaacact atatgggcaa gaatcattat cagggatgat ggctttgatt
    28681 gataaaggac ctgattcatt gggcaaatta acaaaatctc tgaaagattc tgatggtgca
    28741 gctgacgata tggctcggac catgcaagat aatatgaatt cttccatcga gcaaatgttt
    28801 ggagcttttg agtcagcagc tattgtaatt caaaagattc tagcaccatc catcaaaaaa
    28861 gtagcagatg ccatatccgg cttagtagaa aaatttgtaa gtgctccaga atcaactcaa
    28921 aaattagtag tggccatagg agcaattgtc gctgctatag gaccgttaat ttttatgatt
    28981 ggttcagtaa ttatatggat caatagggtg aaagtagctt ttaaagcttt aagtgaaagt
    29041 tcaaaattgt ttagtggatt aagtaaagca atgggtcttc ttacaaatcc ggtttttctg
    29101 gttatagctg cggtagcact actcgttgta ggtttcatct atctttggaa tacgagtgaa
    29161 gattttagaa acttttggat tggcttatgg gagggaatca agtctgctgt aagctcggca
    29221 gtagaatgga ttcagaatgc atggaaatct acaggagaat ggtttaacaa tttatggaag
    29281 tccattaaag aaggcgcaga caatgtttgg actacagttc aagaagcccc tgggaaagcg
    29341 gcagattgga ttaaaaacaa atggactgga acaaaagagt tcttttcaaa tttatggtca
    29401 agtattgcaa actctgcttc agagatttgg aatagtttaa aagaaggtgt catatcagtt
    29461 attgatgatt tagtttcaag tgctggtgaa aaatgggaag ggtttaaaaa tactatatct
    29521 actgcatgga aaacaattac aagtaaaatc aaatctggtt ttgattttat actaaaatat
    29581 attggtccat ttgtaagtag cttttcagat gtgttctcta atatagtgaa agcaataaca
    29641 agtatatttg ctgaggttaa aaacataata gtaaatgctt gggaaatcat taagtcttta
    29701 atagctgcgc cactactgtt tattatagat ttaattactg gtgacttcga acaaatgaaa
    29761 gaggatttag atctaatctg gaacacactt gtccaatcag tggtaaatat ttggacatct
    29821 gtaaaaaata tatttacgga atatatcggt gcaatagtaa atagtgccgt tagtttatgg
    29881 actggattca tacaaagtat ttctaatatt tggaatgaag tagtttatca agcgactatg
    29941 atttggattg atttgaaact attttttact aatttatgga ttgatattaa atacagtgca
    30001 attcaaatgt ggataaatct aaaattctcc ataattcaaa cttggattga tacaaaatat
    30061 ggtgcaattg aactttggaa taatctaaaa caatggtttt tccaaacggt taataatatc
    30121 gtgcaaactc ttataaaaag ttggaacagc ttaaagcaag gaacgataga tttattcaac
    30181 aatacagttc aaggtgctaa agacatttgg acttcattca aatcttggat tggtgattta
    30241 attactggaa ccaaagataa catcattcag ggatgggaaa acctaaaaca aggtactata
    30301 gatactttca acaatttagt aaatggtgct caagaggcat gggataattt agtaaatgct
    30361 gttagtgaca cggttgatag agtaactggc tggtttgata acttgaaaaa tatcgattta
    30421 ctagcagccg gaaaagctat catggatagt tttctagaag ggttacaaaa tgcatggaaa
    30481 tctgtgcaag attttgttgg aggtattggt gattggattc gtgaacacaa aggacctatc
    30541 caatacgata gaaagctatt gattccagct ggtcaggcta ttatgaacgg tctgaataaa
    30601 ggtctgacag gaggattcaa tgacgtacag aatactgttg gaagtatggc agactttatc
    30661 gcggaacttt tcaatgcaaa ttctgatgta gatatagctg caaatttgaa aaatgcaaat
    30721 aaaaacattg gtgcacaagt tgaacataaa ataaatatgg gtggctctac taaaccagct
    30781 gtatttaaaa tcaatcttgg aagacaatcg tttagattgt ttatggacga tatttcacaa
    30841 gctatgggcg aaggtgcaga tattaatctg gaattttagg agggaatatt ttggatcaac
    30901 gagaaaataa aatgtactca ttcaaagata caaccattaa tctcactagt tctaaacgat
    30961 tccttccgac gtctgccatg atgtacgatg gaatgtattt agaagatttg attgatggtt
    31021 atcaaacact tacggtggaa ggtagagaaa tgctttctgt agaagttgaa cagcaagaga
    31081 tacaaattgg ttcaatcatt acaaatcaga aaataccttc aagaacacta aaaataacat
    31141 ataagctgga agatagagat ccagaaaaac tacagtttaa attcaaagaa ctgttgaatt
    31201 atttataccg gaatgaagac gtggaaatta ggtttcatga tgaattagat tattattact
    31261 acggtcgcta tacatcaact gatactgttc caggagactc caactcgatt atttcgagtt
    31321 ttaatgtatt ctgtgcggat ccactaaagt atacgaaaga gtgtgttagt gatggctata
    31381 ttggaaatcc gatacagttt cctataacac caagaaaaat tgaagttact ttatccatga
    31441 ataattcaat caaaattaca aacggagaac aaaatatcac gataactgac gcggcaataa
    31501 aaacaggaga cgtgttggtt tttgattttt ccgatgagca ggtaactgta aacggagaag
    31561 attgtacttc tatgattgat ttagaaagtg attttgagaa cttttatctt aagcaaggtc
    31621 agaagataac tagcaataat gggaagctta aaatattcta taggggggcg acaatttgag
    31681 tgagacagtt tatttctttg atcacttgca aaaacttatt aaaagaaaaa atacaagaag
    31741 tttgattgaa gtctcccaag aaaaagaaat tagttctgat aagagtgatc taatgaaaga
    31801 tactctttac gttacgacaa aatatgataa agaaatagag gatgcaagat atatggcgat
    31861 tcgtgaaaac gagtcgtctt tttcgttgta tcgaattact aaagttagcg acccatttga
    31921 aacattagag tttacagggt taggatttgc gacaaatgaa ttagatgctt acatcatcaa
    31981 agatattagg ccgagtgggc agcccttaaa aaatgttctt gatcgattga ttgaatttac
    32041 tgaaggaaat tggcgcgttg gtcacgtaga agcaatgtta ccaacagtaa ctgcaacttt
    32101 ttactatgtc tctgtaaaag aagcgttgaa agaattgcaa accttaggta tggaatttgt
    32161 ctttaggtgt tctttgaatt ctgatggaat aaaggataaa tggatcgaag tatatgaaca
    32221 aattggcgaa gaatcgaata cacgtttcgt atatggtagt aaagcattaa cagttgtaag
    32281 agagatagat agaagctcaa tctcaacttc aatgataggt cgtgggcgag gcgaagaggt
    32341 tggtgacggt tacggtagaa gaattgaatt cactgatgtt gaatggaaaa agtcgaatgg
    32401 tgatccttta gataagccta aaggccaaaa ttggcttgaa gatccggaat caactcaaaa
    32461 gtatgggata ccacaaaaag atggatcaat gagaaaacga gaaaccgtag tagtgtttga
    32521 tgatatagat gatccaacag atttacttaa aaatacttat tcaaccttaa tcgattctgc
    32581 tagaccgtta gtacaattca aagctgaagt cactggagga gatgtgatag gaaatacagt
    32641 gactattcac agatacgata aaggttatca ctataaaact cgtatttata aaactacatt
    32701 caatcggctt accggtcaaa cgaatatcga actaggggat aatttaacac aagatgttag
    32761 aaaacaaacg gcttctattg tcaataatat taatagttta gaatctagca aaatgacatt
    32821 ttacgaatcg acagagattg gaaaatatca agatgacatt atgcgaggtg caggagataa
    32881 tggcggttct atttattggg taaatggaat tgaagctggt gttagtgata gtagagaaat
    32941 ctatgaaact gtttatatgg atggacctaa cattcctaga tcacgctttt ttatggtcca
    33001 aaataactca gggatatctt tcaaacaatg taaaaaaggt gagtggcaaa caattcaaga
    33061 tgtacacaat ggtgatagca cgaccgcttg gacgttggat ggaactttca atgctaattt
    33121 tattcgcgca ggcattctgt ctggtatcct cgtgcaaggg gtagctttaa aaacatttga
    33181 cgataaagat ttccaattag tggcagaggg gggagaactt tcttttgaaa aacaagtcaa
    33241 gtctactgga ctagatgatg tacatggaga acgcttgggc tcaattgtat ctacttatgg
    33301 ttctaaaggg attaacggct ttgctgtctg gaaagagcca aactatattt tttccattaa
    33361 tgctggggac ggtggcgatc gagggaatcc tatttttcaa attccagcag atgttactgc
    33421 tgataagcgc aaatataatc tttatggtga cggcaatttt tcagaaggaa atataaccat
    33481 agatggacgg ctagatgtaa aagaactcta tgttaacggt acaaaaattg acactaatgg
    33541 aggaggcaat tccggaggga acgataacgg ttggaacgga caatacccac cagaagtaac
    33601 tactgatcgg gataaacgat attggcaaat ttgggcaacg gcaataggtg ccggttttac
    33661 acaacaagct gctgcagcct tacttggaaa tgcacaagga gaatcagatg ctaatccaac
    33721 cgctgatgag ggcaatggcg caccagggtt cggttatggt gtatggcaat ggaccgattc
    33781 ttctggcgca actagcggac gtgtctacat gatcaattta atgacaaagg ctggcatcag
    33841 tgatgatcca gacacaatca cggcacagtt taaattgttg atgtggcatg caccaaatgg
    33901 tcaatggatc gcaactagcg cttatcctta cacatggaca caattcatga atctgaccga
    33961 tatcaacaca gcagcacaag cattcgtggc taactttgaa cgtccacgtg atccacatcc
    34021 agaacggacg gcatggtcac aagaatggta tgacaagttc aaagatttgg aaattcctgt
    34081 atcaaaagga tatatgaagc cgattgcgga tccaatcaca gtgacgagcg aatttggctg
    34141 gcgcacttct ccaatcacag gagcacaaga atttcataac ggtattgacc ttgtaaatgg
    34201 aaatcctaat acacctattt ttgcttcagc ggatggcgaa gtgattgttg caggtgatgc
    34261 aaattacttt gactggtatg gaaattggac agtgattaaa cacgctgatg gaatgtatac
    34321 aggatacgcg catcaaagcc gagtagacgt ctcaaaaggt cagaaggtaa ctgctggtca
    34381 gcaaattgga ctgatgggga caacaggacc atccactgga gaacatcttc attttcaatt
    34441 tatggatgag ttttatccat cttcttcagg tcatttccac aatgcaagag actacatcga
    34501 tttctaaagg agggatagtc atggcagaaa cgcagcataa aatggtccta tccaccaccg
    34561 aaccaaacaa cggaataaat ttggttcgaa ttcggcaagg ggatgtttta acgcaaaagt
    34621 tcgttgttga agtggtggaa catggcaaac taaaaacatt cgagggccta gtgccgtttt
    34681 ttattaatac aacaaaattt ggcgaaaacc aacctgttga acaaaaagta caagaataca
    34741 gtccagcgca agcaaggctt gtttacacct taagtgagcc tgactggcaa tggggtggtg
    34801 aaaacaccgc acatttcagt ttccgatcac ttaatggtga tggaacttgg agtgaacaat
    34861 ttagcacaca ggattttacc tatcgagtca tttctggaat atctagaagc cagttacgtg
    34921 actctggcta tgtgtggaca tttgaggatt tgctaagaaa attcaaagat tacatggatc
    34981 agggcaaaaa tgactgggag cagtggttag aagataatcg tgaaatactg gaaaatatcg
    35041 atccaggtgg tatgatcatt aacattctga atgaagctaa aggcgactac gaatcattag
    35101 ctgatcgttt aaaccaaaaa taccaagtgc cagtcggcag ctcacaaatt agagaaacaa
    35161 cacgcttttt tgattacgac acgatgaaat acgttgacct agtgccgcgc aatttgaata
    35221 cggttgtcaa cagtgttaat aacagcaaat ttaacttttc tttcattaca gacattcacg
    35281 cagataatca caacttgcgt atagatggcg tcggttacaa agatgcttct tatttaagac
    35341 attggcgcgc aatccctcaa tttcaaaaat tagggaacaa aacagatgtg atgatttacg
    35401 gcggagacaa tatcgacggc ggacttggtt cactcggcag cgatataggc attattgatg
    35461 aatggagcgc gcgacattcc atgttaggca cgctcaaacg cttcactaac gcagcggtag
    35521 caggacaaga aaaaccggtt attatctgca aagggaatca cgacgcttgt ttcgaccccg
    35581 cgtggcgcaa gcgaaaggga atgttatgca acgctgactt cgaagagtat tggaacggtt
    35641 tgtacggtgg cgtgttgttc ccagataaaa acgcagcaat ttaccgtttc gatacttgtg
    35701 atttttatga aggcggcaca ggcgacaagt acgtggacgg ttacagcgat acagcgccgg
    35761 gagctttcag tgccaaacaa attaacgctt tcggagaatg gttagtgaac gttccgagaa
    35821 actatcatgt ggcgttagta ggacatacgc ctttaggtct cagcaagttt cccgttcgca
    35881 atgaaaacat gattagcacg ttgatagaag ggtttaaatc tggtagtccg gtaactatcg
    35941 attggtctaa attaggacaa ccgaatgacg gttcgttcgg cggattaaag accttcgcaa
    36001 tgaatacaaa aggcgccggc gttgtagtag gttatttctg cgggcattgg cacgaacaag
    36061 tcgaaggggc gtttggaaca gtaaaaatga ttctttgcga cgtcggcttc tctcatacag
    36121 ctagccaagt tgatacaccg gatgaattag cgttttataa aatagaagtt gacacagcaa
    36181 caagaaaagt gacaagcaaa ggcgtagggc gcgcgagaga cttcacttat aactattaag
    36241 gcggtgaaca attaaatgtt agattttcaa agcaaaccaa atatttttga agaaatgagt
    36301 tatgaagagg ctgtgaaatg gttattgcgt caagcggcta tccattacga cggctcggat
    36361 cacgacgcgc acgtcctagc tactgaaagc aacgcgggct ttgctacacc ggaaacagtt
    36421 atgcaagcgc gtgggcgttg gttacgcgat tataagctac cacaaaaata tccgaacatt
    36481 ttagacattc cgcccggcaa atacgcaacc aaagccggat ggggcgcaga caaccctgac
    36541 gggattgaag atgacagttt tgttgaaatg atggtattcg cggatcacga ttcgagaaaa
    36601 ttaatcgtcg cttttgctcg ttatagcggc gaaatttaca tcaaaatgac acataacagc
    36661 gaaccggtag agggttataa ctcgttaggc tggcggcgcg tttacactac ttctgttctt
    36721 tttgaaggcg aattaagaaa aggacaatca gtcaatctgc cagatgatac tttccgttac
    36781 caaacgcttc gtatccacta cacagacggt gacggagact ttgtagaaga agtgaaacgt
    36841 caaagatacg cgcgaattac aaaagcgaat ttatggaatg gagacgcggg agtgacgttc
    36901 atcgaatttg agctgacaat cgaagcgcga aagataacga tgtcaaacgg cagagcttta
    36961 gatatttcat ccggcaatgt ttccaatccc gcaatgagca acaacgtgaa aataactaag
    37021 attgaaggtg tgaaataatg gcgcatgtca tcaaaaaagg ctctatcaaa gtacctacac
    37081 agccgaaaga ctacgatttg caagcaacgg ggcttgtatt taaatcatac gataatcaaa
    37141 tagcgttaga gttcaacgtc gaacaacaga acggcacacc ggcggacttg ctaggagcta
    37201 acttgcgctt gttgatgttt atctatgatg aagtagatgg aatgatcacg aaagagccaa
    37261 tcccttttat cacgaaaaac ctcatcactg aaagcttctt gaatggacag gtcgtatata
    37321 tcttgccaga agcgatgaaa gcttataacg gtatggtgga agcttatgtt tacatcgagt
    37381 atccagacgg atcaacaagt gataacttag gcttcacctt ccgtatgaag cgttcagcaa
    37441 tcgacggact agcgcaagat aaagcagact actttattgc agactttcaa caattacttg
    37501 atggagtcaa acaagaagca acagatgctg ttaacgcagc attaacaaat gtggattcta
    37561 cgattgaaaa ggcaaaccaa caaataaatg aatttgtaga gggagccaca caagcaattg
    37621 atcaaactgt tgacgaggta acagagcaac tacaagctac tcaaactaag attgataccg
    37681 tttctcaaaa cgttacatcg gcacaaaaca atcttaaagc agttgaagac aagatgaatc
    37741 aaaccaatca gcaaatcggc gacctcggca agctgaaaa
//
LOCUS       DASHHB010000025        37234 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.025, whole genome shotgun sequence.
ACCESSION   DASHHB010000025 DASHHB010000000
VERSION     DASHHB010000025.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 37234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 37234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..37234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.025"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(98..1228)
                     /locus_tag="U2K71_001384"
     CDS             complement(98..1228)
                     /locus_tag="U2K71_001384"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HEM7315475.1"
                     /translation="MSKNRPFTVDHVGSFLRPQSIKEARKKVQEGLLSKEELRAIEDQ
                     EIKKLVEDQKAVGIKGITDGEFRRGFWHLDFLEELNGIEGYVPETGYNQTFHGKAAPA
                     YNIRVVDKLSFNPNHPFLKDFAFLKEVVETDDTFVAKATIPSPTMILRQEILSNDGSS
                     KIKEIYPDLSDFYEDLSSTYKAAIHAFYEKGCRYLQFDDTNWAFLADQTKREALLQKG
                     IDPKEMAHICTEIINNALEDKPEDLTITTHICRGNHASSWLFSGGYEPIAEELFSTNY
                     DGFFLEYDSDRAGDFTPLRHWSNKESKVVLGLVTSKFPDLEDTEKIKERIKEAQQFVP
                     LENLSISPQCGFASTEEGNHLTEEEQWKKIQLLQQVAKEVWL"
     misc_binding    complement(1288..1515)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            complement(1877..3079)
                     /locus_tag="U2K71_001385"
                     /pseudo
     CDS             complement(1877..3079)
                     /locus_tag="U2K71_001385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317927.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
     gene            complement(3139..3342)
                     /locus_tag="U2K71_001386"
     CDS             complement(3139..3342)
                     /locus_tag="U2K71_001386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3173 family protein"
                     /protein_id="HEM7315476.1"
                     /translation="METKCDFMVNRAILIEMGFKPSQAARMIKESKAYLARIEGIDFY
                     NNRQVGVVPSRVIEHLFHIQVAE"
     gene            complement(3484..4716)
                     /locus_tag="U2K71_001387"
                     /pseudo
     CDS             complement(3484..4716)
                     /locus_tag="U2K71_001387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173388.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
     gene            4879..5691
                     /locus_tag="U2K71_001388"
     CDS             4879..5691
                     /locus_tag="U2K71_001388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315477.1"
                     /translation="MEIDKQAVGNRIRQIRQELKLSMEKFGKLIGDLPRSTVNNWERG
                     INLPKTETLHQIAEIGHVTNEYLLYGDQENQYILEMLQKKAGKLDPKIEGLIVDEIKH
                     ADLVSEKEMDRMIEFFITNLIPPTEQDQFTFQCIDEKKQLYLGSTNFGKEAQLYLQHD
                     NQNHILHMMPFTFSNFSVDRILVYLANQKSYSYFGKHLPKELGEKAILLYSINQLTSD
                     VRIAPLVYSEETASYQYTEDNQYLLEQQYLYLPFVMEVEKERLLNAAYPSSK"
     gene            complement(6715..6867)
                     /locus_tag="U2K71_001389"
     CDS             complement(6715..6867)
                     /locus_tag="U2K71_001389"
                     /inference="COORDINATES: protein motif:HMM:NF016526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory gene regulator B family protein"
                     /protein_id="HEM7315478.1"
                     /translation="MRIFTGGVHLNSNILCYFMSVVIVIVAYQCMFIFRNVDFIILGL
                     MFEFFW"
     gene            complement(7058..7936)
                     /locus_tag="U2K71_001390"
     CDS             complement(7058..7936)
                     /locus_tag="U2K71_001390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM7315479.1"
                     /translation="MIYGAIEAGGTKFVCAVGTEELTILERVSFPTTTPVETMKQVID
                     FFKPFKEQLAGIGVGCFGPIDIQCKSTSFGHITSTPKLAWQNFNFVGTLKQYFNIPIE
                     WTTDVNAACYGEYVAGSGKGLAGVAYFTVGTGIGGGALFNGTFVEGFSHPEMGHMLIK
                     RHPKDNFSGNCPFHQDCLEGMAAGPAIEKRLGVKGQNLLADDSFWQIEAFYLAQCAYN
                     TTLMFSPDRIIFGGGVMKQEHMKKKVQDKFVELINGYVEIPPIDSYIITPELGDNAGI
                     IGGLALARKAVRNKQP"
     gene            complement(7975..9093)
                     /locus_tag="U2K71_001391"
     CDS             complement(7975..9093)
                     /locus_tag="U2K71_001391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEM7315480.1"
                     /translation="MSILKNITQFKKIQSRAITPENPTGEKGKAAMLPSELGPSRKGR
                     GAIPLTSGEETVIADITGTGIIRHMWMTIRDRTEFGSFVLRDLILRIYWDGSDSPAVE
                     SPLGDFFCNGFGERCEVNSLPIVVNPTGGFNSYFEMPFQTSAKITIENLHPKDMSSFF
                     YTINYALVDELEENSLYFHANWHRERITKLQEDYKLIDRLKGQGYYVGTYLALTCLER
                     YWWGEGEFKFYIDGDSEYPTVTSTGSEDYFGGAWAFHEYDEFGRPHAKNYSTAFLGYP
                     YQSNRDATRDRFETGKLNAVHAFGDDAMPRHGLYRWHLQDPICFTEDLSVTLQQIGND
                     DVRLFERQDDVASVAYWYSTEASGNTKRHFTDRERLPR"
     gene            complement(9090..11189)
                     /locus_tag="U2K71_001392"
     CDS             complement(9090..11189)
                     /locus_tag="U2K71_001392"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase"
                     /protein_id="HEM7315481.1"
                     /translation="MTINEVIYSPKHPFFNAELNDGNVTTSNEYSYYRNIPDVKGLTF
                     DVSNQKTLANLDVCGTIQTLTFYRENLLTEEKPGVWVNKQLSQTNSLSLKVEVNGIVY
                     HLSESDHRIEVDVIQDCLPRYIHHYSTFKVIVVSFAPILANKRLSMLVQQVYIVNETH
                     ESLQVSVKDVPSYQEKYSDQQNVLISQKGNKDTIAQQEVASFAIAFVDPNAFEEVMTF
                     QESDIEKWLKETLNYFAQLFGQLSMPNDRVVHLYRRALYQSFSSFGMNRQGQVVGSNW
                     GSYPATNRIWNKDMYYASLPFTMFEPELCQKTVLWFDQYGVKFPGSKFTGGINHSLSN
                     SLSSALLASLYFEHTGDTKFFERCPQVLLNASRIIEDILAQRRSGEPMLFPSVWLSDA
                     FALGKYHTGSNLCLWKACIGLAEIFEALEQLSLSKRYKNIACKLKESICKQMTIDGPF
                     GEQFLEGIGDEEKTTYSVKHYQKPILEQGLIFLSDVIVDGKINLLMHDGEESDTTLMP
                     FYQFLDNKDHLYQNTMAFSASSFNPTYSEEIKGITWGLESGATFPGFITVLMSELHNP
                     QAFVTRLEELICLADLDGSWWWWPYRLEAQQGEVVRDFGCGKCGWASGLFVSLMISQY
                     FGLKFKKGVLQIDPVDNLTFSWKNLKFGQAFISIECTQSELSISNLSEKPLKISDTKK
                     ILHVEKGKTIHIQRRKR"
     gene            complement(11240..12061)
                     /locus_tag="U2K71_001393"
     CDS             complement(11240..12061)
                     /locus_tag="U2K71_001393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010755513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM7315482.1"
                     /translation="MKLADGISKDEKKLVRKMFWRSMTMYASVNPVTMGGGGFAYSMM
                     PFINHFYKSEEDRAAALERHTQYFSTTIPCASFVMGIAASMEKENSEKAGFDVESINA
                     IKTSLMGPLAGIGDSLFWGVWRVVTAGLAINLANAGNILAPIIFLVMFNIPNYLMRYY
                     GGVLGYSLGSKYIEKLYANGLIEILTKAASILGLIMVGAMTISMVKFNTVLSFSMKGE
                     ELMNVQEILDSIFKGIVPLLVTLSCFKLLDKKVGVIKIIFGMVVMGILLSLLGIA"
     gene            complement(12058..12867)
                     /locus_tag="U2K71_001394"
     CDS             complement(12058..12867)
                     /locus_tag="U2K71_001394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7315483.1"
                     /translation="MYDAFVVALAVFIGVAGHEMFGMSMLSRPIVVAPLAGALMGDLQ
                     TGLIVGASLEAIFMGVVNIGISSTAEPSLAAGLATAFAIKMGGNVDAIIPIVFPLAVL
                     GLQLMNMIFSFVCGPMAAKFFKFAKSGDQKGIINLHFFMWFVHYALYALIPFFAVLFG
                     ADVVKSVLDNIPEVIMNGLTVAGNLLPAVGMAMLLRMLWSKNIAVWFFFGAVVMAYLE
                     LPLIAIAAVGTIIAIVIAQRDLQLKNISVHAVQSDAAVNATLDQEEEDFFQ"
     gene            complement(12888..13382)
                     /locus_tag="U2K71_001395"
     CDS             complement(12888..13382)
                     /locus_tag="U2K71_001395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7315484.1"
                     /translation="MIKAVRIDERLIHGQVAMTWTKSLNLTGLVVASDEAATNDIQKM
                     TLKMAAPSNVKVIIKTVDDAIHLLKDPRAKDMRLMVLVPNVKDAVKVAKAFPTEIELM
                     NVGNAGKMTPPSEDKKTLSKEVMLTTSEIQDLKELVELYPDTVFQPTPAMEKRSAISI
                     LSDF"
     gene            complement(13394..13882)
                     /locus_tag="U2K71_001396"
     CDS             complement(13394..13882)
                     /locus_tag="U2K71_001396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM7315485.1"
                     /translation="MFLKVGTGIAYIKVNRKKGINHMKKILIATHGHLASGFLSSIQL
                     LTGKVQEITVINAYVDECDFEQELNVFVNQVNEQDQIFVFTDLFGGSVNQKITKTFLE
                     KEIAVTILSGFNLPILLEIVLATKELTKEDIQELVNKCQQELKMTILDEVHVVNEEED
                     FF"
     gene            complement(13959..16655)
                     /locus_tag="U2K71_001397"
     CDS             complement(13959..16655)
                     /locus_tag="U2K71_001397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM7315486.1"
                     /translation="MEYKYRIDILQPIVYYAFRHIKLEEKMKKSAKDRVFDELIRLTG
                     NMSSVTAEKISKNLGISRQNASHYLTRLVEDKKVEKLPGKPVLWKPLDEYAVVDNTEQ
                     INEAFHSVVGHDGSLREVIQKCIAAVKYPPNGLSVLINGATGVGKSFLATKIFEYAIH
                     EQIIEKDAPFAILNCADYADNPELLSATLFGYKKGAFTGAEKDTDGLLATANNGYLFL
                     DEVHRLSKENQEKLFLFIDTGNYRPVGENVNWHSAKVRFIFATTEKGENYLLDTFDRR
                     IQISVMLPTFEDRPIRERLELIQLFFQNEADVLQKDIIVSREALSLMLSHPFSGNIGK
                     LKNIIKISCADVYSRTQEEPLTIGKNEILIQLNMLESEVESLPIASLLIKKNQKIKTD
                     RIKPFDYDSILEKITQLINEGNFNENFSSIKNEVQHLVHHIDKDSFLSMSRKLMIQMF
                     RKVWVSVLGKTYGLTTSGPVAEIISRSYVISPSYKTDLTGINRTLAANLPRTTYLCNQ
                     FLEHLPPLSEKDENFLRLLIVVTLSDYVDESIELKGLLLAHGDSTASSIQAVVNQLCG
                     NYVFEALDMPVESNVSEIVESTRNFVKRQVNTDSLVLIVDMGSLNQIYTQIKNDLKGE
                     LLLLNNLTTSIALDIGLKMTMNAPFKQIAEDAADKYSINVQYFEGFSQSSNIIISCMS
                     GLGISDKLKDVFRHHLSDESIEVFTKDYRELRELIDQNDETYFDKTKLVITTSDLPRS
                     FSIPNINVYDILDGDGAANLEHILSPEMKPAEFNRLIQELVKFFSLEGIADRLNFLNP
                     TIVMSEVETVISKYENYYHFTLNGRVKLNLYMHTALMMERLFLARTEKSEEPNYDLKP
                     DEDEFYYVSRSIFQSMEMKYNFKVNAYELSLLYELLGPYIQK"
     gene            complement(17083..18156)
                     /locus_tag="U2K71_001398"
     CDS             complement(17083..18156)
                     /locus_tag="U2K71_001398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM7315487.1"
                     /translation="MKKLQSLFLGILIIIAVLLLGVRQLEKTSGMSGAKVLTIYNWGD
                     YIDPSLITKFEKEYGYKVNYETFDSNEAMFTKIQQGGTNYDIAIPSEYMIQKMIKEKL
                     VLPLDHSKIKGLNNIDPRFLDLDFDPDNQYSIPYFWGTLGIVYNDKFFDANQIKHWDD
                     LWKPELKDSLMLIDGAREVMGLSLNSLGYSLNSKNMAQLTEAANKLNHLTPNVKAIVA
                     DEIKMYMINEEASVAVTFSGEAADMMWENEHLHYVIPPEGSNLWFDNIVMPKTVKNKE
                     GAYDFINFMLEPENAAQNAEYIGYSTPNEEAKALLPKDISSDEQFYPSDDTISHLEVY
                     KDLGSKYLGIYNDLFLEFKMYRR"
     gene            complement(18153..18980)
                     /locus_tag="U2K71_001399"
     CDS             complement(18153..18980)
                     /locus_tag="U2K71_001399"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315488.1"
                     /translation="MKKFKWSYLYLGIVFLFLYAPIFYLIFYSFNEGGTMNSFTGFTW
                     ENYEAVFEDTRLIIIVLNTFMLAFLSALIATIIGTFGAMGIYYTKKRRARTALLSMNN
                     ILLVSPDVIIGASFLIFFTLLGFGLGFQSVLLSHIAFSIPIVVLMVLPKLQEMNDSMV
                     DAARDLGANNFQVVKNIILPYLSPGIIAGYFMAFTYSLDDFAVTFFVTGNGFTTLSVE
                     IYSRARQGISLEINALSALVFLFSMILVIGYYFISKENRPKRLKKNRRVREGVARLR"
     gene            complement(18981..19787)
                     /locus_tag="U2K71_001400"
     CDS             complement(18981..19787)
                     /locus_tag="U2K71_001400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315489.1"
                     /translation="MKTMRRIYSVPYILWLGLFVIAPVLMIIYQSFFDMNGQFTLNNY
                     LSYFTSGTSLSMTINSVWYAFLITFFTLIISYPTAYFLTKLKHKQLWLMLIILPTWVN
                     LLLKAYAFIGIFSVHGSVNHFFDFIGIGPKQILFTDFSFLFVATYIEIPFMIMPIFNA
                     LEEMNPSLISASRDLGANSFETFRRVIFPLSLNGVKSGVQAVFIPSLSLFMLTRLIGG
                     NRVITLGTAIEQHFLVTQNWGMGSTIGVILIIAMFIVMLLTGEKKKGGRS"
     gene            complement(19799..20884)
                     /locus_tag="U2K71_001401"
     CDS             complement(19799..20884)
                     /locus_tag="U2K71_001401"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315490.1"
                     /translation="MGKPIISFDQVVKRYDDETVLKKVSFEIEQGKFYTLLGPSGCGK
                     TTILRIIAGFTEATEGDIYFEGKRLNDLPANKRQVNTVFQDYALFPHMNVFDNVAFGL
                     KIKKMSKSDIEKKVKDALRMVQLPGYEKREISEMSGGQRQRVAIARAIVNEPKVLLLD
                     EPLSALDLKLRTDMQYELRDLQQRLGITFIFVTHDQEEALAMSDEIFVMNKGKIVQSG
                     TPVDIYDEPINHFVADFVGESNIVDGVMIEDNLVEFVGKQFECVDGGMRLNEPVEVVL
                     RPEDLTITTPDKGKLVVTVDTQLFRGVHYEIICYDEQQNEWMVHSTKKAKEGSKVGLA
                     FEPEDIHVMRFNESEEEFDARLDSYEE"
     gene            complement(20901..21443)
                     /locus_tag="U2K71_001402"
     CDS             complement(20901..21443)
                     /locus_tag="U2K71_001402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315491.1"
                     /translation="MEIGEKLRNLRIQKNLTQEELGERTDLSKGYISQLERDLSSPSM
                     ETFFSILEVLGVTPEEFFHQEAANLQVVYSKEDHTVYFDEENGYELEWLVADSNEKEM
                     EPVLLTFEGAGEYKTFEPSLSETFIFVLEGELELSLGETVYVAKAGQSIYYQATKQHQ
                     LRNHLKKRTKVLIVATQSYL"
     gene            21701..22690
                     /locus_tag="U2K71_001403"
     CDS             21701..22690
                     /locus_tag="U2K71_001403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidoreductase"
                     /protein_id="HEM7315492.1"
                     /translation="MNAFRSFQVIKEPVFHTAISDLPFTELAENEVLVKIAYSDVNYK
                     DALAMSESGQVIRTYPMTPGIDLSGTVVQSNNPRFSKEDLVLATGFGLGVTHPGGYSQ
                     YQKVPGDWLVPLPKNMTLRQAMILGTAGFTAMLCVNALIRQGMTSDKKVVVTGASGGV
                     GSTAIALLHKLGFQSIIAFSRKEESVTWLKSLGALQVVRPDEFLPETTKALGKQQIDY
                     VIDTVGGEQLSSLLPLISYNGAVALCGNAGGIKLNATVLPFILRNIQLIGIDSVNVPI
                     DQRLSLWQQMADLQIADELVVQEITLDQLPETASKLLAGTHQGRTLVNVGDHK"
     gene            22687..23424
                     /locus_tag="U2K71_001404"
     CDS             22687..23424
                     /locus_tag="U2K71_001404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014386989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenate--cysteine ligase"
                     /protein_id="HEM7315493.1"
                     /translation="MKILITAGGTTEKIDQVRAITNHSTGRLGQALADYLAANPDTTI
                     DYVTTKQALKPKRHSNITIYTIESALDLFLQLEELTKKEHYDAIIHSMAVSDFTPAFS
                     FSEEQLAKNLPTSSTQEELDNWFAENEQTDTTVSKISSNTEHLVLVLKKTPKIISYLR
                     EWQPKAKIIGFKLLVDVPKESLLAVAKNSLINNKTDFIFANDLTEIHGETHHGYLLSK
                     DGTVEEAQSKSEIAALITEKIRLEESK"
     gene            23421..23972
                     /gene="coaC"
                     /locus_tag="U2K71_001405"
     CDS             23421..23972
                     /gene="coaC"
                     /locus_tag="U2K71_001405"
                     /EC_number="4.1.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantothenoylcysteine decarboxylase"
                     /protein_id="HEM7315494.1"
                     /translation="MKKILLGVTGSISAYKSADLTSQFVKLGAQVDVIMSKSSTHFIT
                     PLTLQSLSKRAVHTDVMQESNPQVINHIELAKQADLFLIAPATANIIGKLANGIADDL
                     LSTVAMALPVGTPKLIAPAMNTNMYQNPITQRNLETLRSVGYQEIEPRESLLACGDFG
                     KGALADIQTIIEQVMLTLNERNA"
     gene            23988..24620
                     /locus_tag="U2K71_001406"
     CDS             23988..24620
                     /locus_tag="U2K71_001406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM7315495.1"
                     /translation="MKNTKNFTLTAMFLAILILLAVTPLGFIPIGPINATTMHIPVII
                     ASIVLGPRLGAFLGGTFGLISMIRSTVIQTPLSFVFSPFIPVIGTEHGSWKALLIAFI
                     PRILIGVVPYFVYKGLRKLMKQKADSVSLFLAGLSGSLTNTILVMNMIYFLFQQDYAK
                     VIGTNLNAVYSAILAVIFTSGIPEGIVAGLATAAVCNVLLRYFKHPSLTN"
     gene            24705..25352
                     /locus_tag="U2K71_001407"
     CDS             24705..25352
                     /locus_tag="U2K71_001407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM7315496.1"
                     /translation="MKIFVVGANGQIGRHLIKDLAPSSHEIFAGVRDVASQTLVKKEN
                     VSYVSFDLTWSVEKMTEAFKGIDVLIFAAGSQGKNLLQVDLDGAIKTVIAAENAHVSR
                     YLMVSAVYADEPAKWPESMTDYYITKHYADEWLKRTNLDFVILQPVTLTNDEEVTSIQ
                     LTKPNEKASKTITRSTVAAVLAALVEETDISRTTLVLSEGSKELNTAFQEWAKEE"
     gene            25357..26340
                     /locus_tag="U2K71_001408"
     CDS             25357..26340
                     /locus_tag="U2K71_001408"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HEM7315497.1"
                     /translation="MRAISIKHPGSVDVLEQIEVPVPKPKQGELLIKVHTAAVNRTDI
                     MKRESVSLKPPYPILGVEVAGEVVENKSDISTFTPGTKVYGLVNLGGYAEYAVMPADR
                     AILLPDTLDYVSAAGISEVFLTAYQTLYWLGKLQEGETVLIHAGASGVGTAAIQLARQ
                     LSHAKIIVTAGSEEKLAFCKELGADELINYKKQDFQEEVRKITEGRGVDVILDFIGAS
                     YWEKNLASIAVDGRWVLIGMLGGTIVPEIDLGVLIGKRVQLLGTLLTPRSDQYKAELA
                     QTFMQKAGPFLENGKIRPIIDRTFQFSAVKQAHEYMEANKNIGKIILKITE"
     gene            complement(26743..28257)
                     /locus_tag="U2K71_001409"
     CDS             complement(26743..28257)
                     /locus_tag="U2K71_001409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavocytochrome c"
                     /protein_id="HEM7315498.1"
                     /translation="MKKLVAGLLTAVALLAMSACGADQKNTAQTSSSTKESAEVSSGA
                     SEQVYTDPKEMKESYDVVIIGAGGAGMAAAIEAKDAGLNPVILEKMPVAGGNTSKSSS
                     GMNASETKFQKEEGIKDSNDAFYEETLKGGGGTNDKEMLRYFVDHSADAVDWLDQNGI
                     KLDNLTITGGMSEKRTHRPSDGSAIGGYLVEGLLNNVHEREIPIFVNADVTDIKKNGE
                     TVNEVTVHVQGEEPKEVTGKAIVVTTGGFGASKEFIEKYRPDLADYITTNQEGSTGDG
                     ITMIEKLGGQTVDMDKIQIHPTVQQDEGVLIGEVVRGEGAILVDQEGKRFVNEMDTRD
                     KVSAAITALPEKSAYLIFDQGVRDRATAIEFYDQKGYVTTGKTMEELAEKIDLPKETL
                     AKTVDGWNTDVAEKKDAQFNRATAMEHDLSSPNYYAIKIAPGIHYTMGGVKINTNTEV
                     LDKENQPIRGLYAAGEVTGGLHGNNRIGGNSVAEIIIFGRQAGIQAARFASENE"
     gene            28395..29201
                     /locus_tag="U2K71_001410"
     CDS             28395..29201
                     /locus_tag="U2K71_001410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315499.1"
                     /translation="MNHLKTFFTNSTKTAGRITNGIYILLRIIVVIMLVDQVWNQHWG
                     NTMLLLLTLILFEIPYFLEKILKIQIPNMLEIILLSFIFSSTVLGELSDFYGYFSMWD
                     TFLHAFSGFLAGGVGFSLVYLLNKNTKTMNLTPLLLAVVSFCFSMTVGVMWEFVEFSA
                     DKLLQFDMQKDTIIQQINSVELSKDGNTVYHVPDITRTIIESQNADGKTDKTIIQNGY
                     LDVGIMDTMKDLFVNFIGAVVFSVFGYLYSRNDYQKFTFIKNFIPKKIKR"
     gene            complement(29376..29945)
                     /locus_tag="U2K71_001411"
     CDS             complement(29376..29945)
                     /locus_tag="U2K71_001411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7315500.1"
                     /translation="MLDKTLPYAEIWMTRPLQQNVPITPLAEGFEFRFYQKEDESAWA
                     EIETSVGEFQTTDEALEYFQQNFAPYPEELERRMFFVETKKGEKVATCTAWRKKSREQ
                     VYPVFHWLAVKPDYQGKGLAKALTARVLQEFPTLHTDGPIYLHTQTWSHQAIALYKKM
                     GFTFIAENLDGTPNPDYEKVMQVLSKLDK"
     gene            complement(30028..32088)
                     /locus_tag="U2K71_001412"
     CDS             complement(30028..32088)
                     /locus_tag="U2K71_001412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacterial Ig-like domain-containing protein"
                     /protein_id="HEM7315501.1"
                     /translation="MCSKRRSKQLNKHACRLDKERSCSEKHYVKFMMRTMLISLSVGV
                     YMHVPVQATTQTIKVVRTSVSETTDSPITITHTFTDANGKQLLPALTSYNKVSYPPTI
                     NHYWLIPNKTLWTSETGSSVPLSIFSDMTDPTKGDQLKQITDILNHALTDIKDGGSAQ
                     VSYVYEKDYSQFERKKIDVLAGTKVSISDLVERLKNADGTQGDMSKVISNNGNIVDTT
                     NTGTSTISLSYFDPIALKTMTASAVIHVRADQTAIKGKDLHLRTGESYAPKDLVEYVT
                     KSDGTSGNKEEIKMNGTVDTTTPGEYPVVLTYIDSKTSNVAETTAVVYVEASAASSTV
                     ITHFIDSQTNKELQAETHMSSVNRIPDLRLPAEFSDRVPNIDLSTYEQNGKVTSISDW
                     MQANGLDRKSHWSEILEVLHSELMNNRKQSGGEITFTYVYIPDNRSLEGHDLIVHPNE
                     KINKEELIQSATNSLGKPVPLKDVSVTINEVPLEDGYLTKQTGDFTIMYSYHDPYSLQ
                     ELHAQSLLHVLPLNKQIDQKEQKRQEESLSEKKQQMAHAVSPTGKVKQTRSLELARIS
                     RKISESNKTIRQEAHLAPQVSGLENEILEPKIKQQSEKEFVAKQSEDQKESDDQKETK
                     KKRLSTIDSTDEHLQPQVPLPSPGTPAPGGGIPSSALGDFMRGMAGTWVYANRNEFDA
                     DDWF"
     gene            complement(32365..33843)
                     /locus_tag="U2K71_001413"
     CDS             complement(32365..33843)
                     /locus_tag="U2K71_001413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315502.1"
                     /translation="MKLDYLIEEEDREKLLLARYLEFEKKNYVVLPKLYETLGLSKFK
                     TRNYIIELNKEFTEFKTKPEIQISEEAEIHVERLNLTDIKNLRRIYFQRSKIARLLTE
                     IVDSKVTIEKFAENHFLSISRAYVKRRELVSFLEQHQIKLKKNILVGEEINIRNVLFG
                     VYFGVFNGFSMPFRESVMNEAKSIVQYFEYLFNLKLSATKRIKFNLLTTISLLRAYGN
                     QPIGKRFFSDEYFKSSEGMKIIKDLSHISFLHIPKEQIIDEISYIMLFLITEEEFELD
                     GAFGLAEAHFHDIHMISQKMSVELIDQLPFSIEISEEERLYIRKELRMGMMHVNRKNW
                     IFDFEMTSFTSKKQLQFFFETYPIFSTAIQKVIQENKRYFLGKDERFMIRLFYDYLFL
                     LVGICPVSYFEAPIHVCIDFSQGSAYTDYIISQVKGFKNYNIVIESRITSRTNIYLSD
                     CLLEKFNKEQIIWKNPPTPDDWEYFGNTIIREKNRHLTEKIS"
     gene            complement(34141..35076)
                     /gene="murB"
                     /locus_tag="U2K71_001414"
     CDS             complement(34141..35076)
                     /gene="murB"
                     /locus_tag="U2K71_001414"
                     /EC_number="1.3.1.98"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287058.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate dehydrogenase"
                     /protein_id="HEM7315503.1"
                     /translation="MNKEEIVESLATITILFDEPLMNYTFTKTGGPADVLAFPKKQEE
                     VKQIIDYCRIHDIPWMVLGNASNLIVQDGGIRGVVIMLTEMKQIHVKGTMVIAEAGAS
                     LIDTTYAALAESLTGFEFACGIPGSVGGAVYMNAGAYGGEIKDVFAEVDLLLEDGTLK
                     TLTKDEMVFSYRHSKVQELRAIVLEARFSLQTGDYEAIKARMDELTELRQSKQPLEYP
                     SCGSVFKRPVGHYTGQLIQQAGLQGLKWGGAQVSEKHAGFIVNIDHATATDYIELIAH
                     IQEVIKEKFDVSLETEVRIIGEKQAISETSRSLAN"
     gene            complement(35149..36069)
                     /locus_tag="U2K71_001415"
     CDS             complement(35149..36069)
                     /locus_tag="U2K71_001415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HEM7315504.1"
                     /translation="MKIGVIGVGNIAEKAYLPTYAKKQGTLDFYFATRNKETKERIQK
                     EYGFQHLYETIDELLEEKIEACMIHAATKVHYELAKKCLENGIHVYIDKPLSVHLNEV
                     RELQELADKNQIILMIGFNRRFAPMVEELKRIPDKRLIHLQKNRIAAKETTEFVLYDL
                     FLHLIDTAVYLLDEPILRTKYQIRETKEFLEYAILQLETQNQTAILTMDLASGANIEK
                     YQVTSKNGTYEVNDLTQLVIQDADGKKVKEFGDWTNTLVKRGFEPMVEAFFSQIRLGK
                     SETELLKQKEVVRSHELCEEVLKDHYRHQL"
     gene            36182..36934
                     /gene="xth"
                     /locus_tag="U2K71_001416"
     CDS             36182..36934
                     /gene="xth"
                     /locus_tag="U2K71_001416"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HEM7315505.1"
                     /translation="MKFISWNVNGLRAIVNKNFLEVFHELDADFFCLQETKLQAGQID
                     LDLPGYYQYWNYAERKGYSGTAIFAKKPALNATYGMGIDIHDTEGRLITLEYSDFFLV
                     TCYTPNSQSELKRLDYRLEWEEAFYNYLENLKKQKPVIVCGDLNVAHQKIDLKNWKTN
                     QKNAGFTPEERAALSRLLDNGFIDTFRYFYPTQEGVYSWWNYRFNSRKNNAGWRIDYF
                     LTSKDLEPGLADAKIHTDIMGSDHCPVELDLK"
BASE COUNT        11142 a         7707 c         6288 g        12097 t
ORIGIN      
        1 aataaataat ataaaaggta aataaaaaag agtggatttc tccactccat gaaaagccga
       61 ctttttggga cgcttttttt tgccaagcct cacattttta tagccaaact tcttttgcga
      121 cttgctgcaa taattggatt ttcttccatt gttcttcttc tgttaaatgg tttccttctt
      181 ctgttgaagc aaacccacat tgaggactga tacttagatt ttccaatggt acaaattgtt
      241 gtgcttcttt tattcgttct tttattttct ccgtatcttc caaatctgga aacttagaag
      301 taactaatcc taaaacaact ttactttctt tgttactcca atgtcttaaa ggagtaaaat
      361 ctcctgctcg gtcagaatcg tattccaaaa agaaaccatc ataatttgtt gaaaacaact
      421 cttctgcaat gggttcatat ccaccggaaa acagccaaga agaagcgtga ttcccgcggc
      481 agatatgtgt tgtgatcgtc aaatcttctg gtttgtcttc cagtgcgtta ttgataatct
      541 ctgtacaaat atgggccatt tctttcggat caattccttt ttgaagaagt gcttctcttt
      601 tagtttgatc agctagaaac gcccaatttg tatcatcgaa ttgcaggtaa cggcaacctt
      661 tttcataaaa agcatgaatc gctgctttgt aagtagagga caagtcctca taaaaatcag
      721 atagatcagg ataaatctcc ttgatttttg aagaaccatc attagatagg atctcttgtc
      781 gtaaaatcat cgtgggacta gggatcgttg cttttgcaac aaaagtatca tctgtttcca
      841 ctacttcttt cagaaaagca aaatctttta aaaatgggtg atttggattg aaagataatt
      901 tatctactac ccgaatatta taagctggtg ctgcttttcc gtggaatgtt tgattatatc
      961 cagtttctgg aacatagcct tcgataccat ttagttcttc taaaaaatca agatgccaaa
     1021 atccacgccg aaattcacca tctgtaattc ctttgatgcc gactgctttt tggtcttcca
     1081 ctaatttttt tatttcctgg tcttcgattg cacgaagttc ttcttttgaa agtaagcctt
     1141 cttgaacttt ttttcttgct tctttgatcg attgcgggcg taagaaactt ccgacatgat
     1201 ctactgtaaa tggtctattt ttgctcattt cactcactct tttctttgtt ttttgtaaaa
     1261 aaaaatcctt ttactagcaa atactagtaa aaggacgatt atactcgtgg taccaccttt
     1321 tttcagaaaa aacattcttt ctctcagcct gacaataaaa tcagtggtct tggtaacgaa
     1381 agactgttcg tttttgccta cctttcgaca aaaaatcctc taaggccatt ttcgacagtc
     1441 aacttctagc tttttacacc aaccaaagcc tctcttgaac cattttctgt ctactttcct
     1501 tctcatcgga tactattaga ataaaattag aatagcatgt ttgaaagaaa agtcaatagg
     1561 gaataagaac aaacaaaaac taatttgtta ttcttagtaa tttgtttgtg gaatacaata
     1621 aagcttttct aatatgataa ttgttcagtc gtcctttcat cttagatcac ttccttaaaa
     1681 tgatcatacc cgtttcaacc acataaaaaa ggacttaatt ttgaaaaatt tcccatcaaa
     1741 actaagtcct ttttattata gttatgcaaa tttgcttatt ttctcgctat ttccgataca
     1801 tcttgaacag gagcaattcg actgaaaaca ctggtgcaaa agcatttatt tgtcttatgg
     1861 tagaaccgtg gaaaactcag actggtaata aattgtcatt ttttgaaaga atagcgccct
     1921 cttgaacatt atcaatcgca cttaccactt gttgatctga ctttgcatat aagtgaccgt
     1981 aagttcctaa agtgatttga atattcgcat ggcccaatcg ttcttgaata gatagcggtt
     2041 gaatattctg ctcaattaga aatgcaacat gagaatgtcg tagcgcgtga agcttaatcg
     2101 gtttgacacc cgctttttta gcagcttctt ttaaccagtt cgcaaaagaa taaggtggcg
     2161 taaatgttcc gtccaactga gcaacatatt caaaatttcc aatacgtttt tgcaagtcat
     2221 accactgtct gagttttaag gaaagcttct tccccaaccc aatcgttcga taagcacatt
     2281 tagttttggg agtcgaaaag taccaatcat cttttgtatt ataaatcaat gtcttctcaa
     2341 tcgttatcgt gtttttctca aaatctacat tcgaccaaga caaagcttca agttctccaa
     2401 tccgaatgcc agtataaaag agcatttcat acacaatctt ccgatggtgg gtttgaatct
     2461 tatcatcctt caattcattc atcacttttt gaaactcact gtgttcccaa aactcaactt
     2521 tcttattctc agaaggaagc gaagaaacat cttcaacggg atttttagca atcaccccct
     2581 ctttaacagc ccgatccaat acaattttta ggtgccctcg aatccgattg atataagatt
     2641 ttgataacgt ttcgccatta acttttttcc gtgaattacg tgactctgtc tggcttagtt
     2701 caaataacca atcctcaata tcgtgacttg taatatctcg aacacgaaaa tgatagaaaa
     2761 actgcatacg cttttcgaaa atgaattgcg cattctcata tgtttgactt ttgacctgtc
     2821 tctcgtacca ttttacaaac ctatccttgt aaaactcttc gaaagtcatt tgattggtag
     2881 aacacagttg attaggaatt gtcttgttaa aatcatgtgt ttccatgtac ttgtcataag
     2941 caattttggc atctttttgt gaagcataac ctcgtttagt cttctgaacc tttcttccat
     3001 tttcatctat acctagatac gctcggaaga actatttttt tgtcttttta tcttggtaaa
     3061 tatttttaat ttttggcatc gttccattcc tccctgtact tgagaaaagc tactttaata
     3121 gtaccgcgct tgtactgttt attccgcaac ttgaatgtga aaaagatgtt caattactcg
     3181 ggatggcacc acaccaactt ggcgattatt ataaaaatca ataccttcaa tccttgcaag
     3241 atatgccttg ctctctttaa tcattcgtgc agcttgacta ggtttaaagc ccatctctat
     3301 caaaattgca cgatttacca tgaaatcaca ttttgtttcc attctctcac ctcctgattc
     3361 tttactcctt ttaaaataat cctctttata atctattgtt tctcattcga tggttgtgtt
     3421 atactcgtct tgtcgaggcg gagtacgttg cacaacttgt tgtgtaagca gtactttgct
     3481 tttttagtag cttgttttct catttgatat actttgtaat tttcgttcat aatactcaac
     3541 ctcctcctga taatactccg ggtctgcctt catattttgg agaagtttga tctgtcttgg
     3601 attcatttct ccttgttcaa taatcaaagg caacaaggaa gaatgaaacg tttgatcgac
     3661 ctgtgcaata aaaactaatg atggtgaaac accatgaatt aaccaactca tagttttctc
     3721 aaaactttga ggaattgtat ctaaagaaat ggtgattgcg ccataattgc taataaaggc
     3781 tgcccatttg gcatcaactt ttgcacctag atcatttgga tcacggtcat aaaaacaaat
     3841 tgctccatta agtaactcaa agaacagttt ggttaaatca tgggtttttg aaataatctt
     3901 tgccaagctt tgcgctttct cttttcgata ccgtagttca aagcgattct ttacttttgc
     3961 ctcttctaaa ggaattcctc gtcgcttccg ttgttcatgg tccttctgat aaaagcaaaa
     4021 acgacactga ctcttctttg agcctaaata caaggtctca ccatttgatt cattgtttat
     4081 gatatccgta ccgccatgag attcactcac ttgaaaagtg gtccacacat ggcctagtat
     4141 taccttatgc ttaagttctg gaatgctgag actccccaca aaatcatcta aagtaaaatc
     4201 aatccgtgta aagttcccct gataatctaa taccttacga aaaaactgtt gccaatttgt
     4261 ttttcgtgtt tttagccatc ttgctaagtg tttacagcct tgacctgaaa actccatcac
     4321 agttccttta accgcatcgt caatggaata ccgaatcgtg attacttgat tccacacgta
     4381 ttggccaata tagccctttg gcgcataatc cagttcctca aaacgttcaa tgggcatttc
     4441 aataatctct tcaattagct ttaaatgttg attcgagaaa aagtgaatgg ttagataatc
     4501 aatcaaaaca cggttaactg gaacgacctc ttcttgcaaa ctatccaaca caagattcaa
     4561 tttttcttca cttaattgtc tcgttccttt ttctactttc gataataact atctcgaaat
     4621 ccccactttt tctgctaaat atcgttgtgt ccaccccctt tcttctcgag aaactcgaat
     4681 ggcttcaccg agatcatgtt gattaacttg gttcatcgac aaacctcctt ctgctattta
     4741 cttgtcaaag aacctattga acttatttta agtacatttt tattgtacgc ctgtatttat
     4801 tttaagtcaa tatgttttga ctttttttaa gtacacacgt atactaaatt taagaactta
     4861 tcctgaggag gagttttcat ggagatagac aagcaagcag tcggcaatcg aattcggcag
     4921 attcgccaag aattaaagtt gagtatggag aaatttggca aactaattgg tgatttaccg
     4981 cgaagtacag tcaataactg ggaacgtggg atcaaccttc caaaaaccga aacccttcac
     5041 caaatcgcag agataggtca tgtcaccaat gaatatcttt tgtatggtga tcaagagaat
     5101 caatacattt tagaaatgct ccaaaaaaag gcaggtaagc ttgatcctaa gattgaaggt
     5161 ctcatagtag acgaaatcaa acacgctgac cttgttagtg aaaaagaaat ggatcgaatg
     5221 attgaatttt ttattacaaa tctcattcca ccaactgaac aagaccagtt tactttccaa
     5281 tgtatcgatg aaaaaaagca gctttatctt ggttcaacta attttggtaa ggaagcccag
     5341 ctctatttgc aacatgataa tcaaaatcat attctgcaca tgatgccctt taccttctcc
     5401 aactttagtg tggatcgcat actcgtctat ttagcaaatc aaaaatccta ttcctatttt
     5461 gggaaacatt tacccaagga actaggtgag aaagccatcc ttctatactc aattaatcaa
     5521 ttgaccagcg atgtgcgaat tgccccgttg gtctattcag aagaaaccgc ttcttatcag
     5581 tacacagagg acaatcaata tctactagaa cagcaatatc tttatctacc attcgtgatg
     5641 gaagtcgaga aagaacgcct gttaaatgca gcgtatccat catcaaaatg aggtgacaaa
     5701 attgataagc acgtgacaaa gaaaatactg ctaggacact ccccaatggg tagttgtcac
     5761 agtttcctgt tctttgttag ccccctgtta gaaccggggg ctttattcct gcagtccggc
     5821 tacgccttcc tgcaatagac aaaactccag atcgggtaat tcgcttgtgg cgaaacccga
     5881 cctggagttt gtctctgtat tttgaaccct aaaaattaat caaacagact gatattctca
     5941 taatcagcgc gaaataagcc attaagtaca gttttcctac ctatttttga taacccgctg
     6001 gttagttaat attccttttc cgagagagat aaatgattgg ttaaatctat atacatctca
     6061 acctttaaaa atgttttcga aatacttcat cagtacatgt catacaaatt accagtgcat
     6121 tctcaaattc tagttcttta attgatttca gttgaggagc agttactttt ggcggaaatt
     6181 tccatttttg tccaaacctc ttcccccgat gaaatttctt tgttgcaatt attgcaatga
     6241 aaaatttttg tttcaaaaat atacataatt acttcctcca ataaaaacta tctacctctc
     6301 aaattatttt tctctaataa gaaaaacaga aaaggtaata agatacaaca gtgatgattc
     6361 ctaataaaga aaagactaca ataatatttt tctaacttat cgccttcaaa gaaatagttt
     6421 cttatttttc actaaaataa aaaatttatt gcaaaaaaaa tagaagtgac aaaattatca
     6481 atcatttcaa ggtaatataa ctacctgagt ttattatcga ttaaaatgct aactacattt
     6541 gcaagagaag cgagagttaa aggtaaatat agaatttcta cttttttagc aatagaaata
     6601 ggaataaaat atttaaaatg tatagaaact ttcttttttt ttcttgctat ctttttataa
     6661 tcacaactct ctataataga attacgattt attttatacg gctgaaaaaa tctatcacca
     6721 aaaaaattca aacattaagc caaggattat gaagtcaaca tttcgaaaaa tgaacataca
     6781 ttggtaagca actataacta ttaccacaga cataaagtaa cacaatatat tactatttaa
     6841 gtgtacgcca ccagtgaata tcctcaaggt atacagcgca caacaaaaag aaaaaaggaa
     6901 acgcagaagc atttcctttc agtgatttaa tgaaaacaac acgatttgac ttagagcctt
     6961 tttatttgaa ttcattttcg ctttatccaa cttcatttga tcatttgatt actcggttaa
     7021 atacttttga catgagtatc aatgaaacat tcattaatta tggttgctta tttcttaccg
     7081 cttttctggc caatgctaat ccgccgatta ttccggcatt atcacctaat tcgggcgtaa
     7141 taatgtagct atcaattggt ggtatttcaa catatccatt tataagctca acaaatttat
     7201 cctgaacttt tttcttcatg tgttcttgtt tcattacacc accgccaaaa ataattctat
     7261 ctggcgaaaa catcagtgtt gtattgtatg cacattgggc aaggtaaaag gcttcaatct
     7321 gccaaaacga atcgtcagcc aataaattct ggccttttac acccaaccgt ttttcaatcg
     7381 ctggtcctgc tgccatcccc tctaaacaat cttggtggaa gggacagttt cctgaaaaat
     7441 tgtcctttgg atgtcgcttg atcaacatat gccccatttc cggatgactg aacccttcca
     7501 cgaaagtccc attgaacaag gcaccacctc cgatacctgt tcccacagtg aagtatgcaa
     7561 cacctgctaa ccctttgcca gaaccagcaa catattcacc atagcaagcc gcattgacat
     7621 ctgttgtcca ttcaatcgga atattgaaat attgctttag cgttcctaca aaattgaaat
     7681 tttgccaagc aagttttggt gttgatgtga tatgaccgaa actcgttgat ttgcattgaa
     7741 tatcaattgg accaaagcag ccgaccccga tgcctgccaa ttgttcctta aaaggtttaa
     7801 aaaaatcaat gacctgcttc attgtttcaa ctggagttgt tgtgggaaaa ctgactcttt
     7861 ccaaaatcgt taattcctca gtaccgactg cacagacaaa tttagtccct ccagcttcaa
     7921 ttgccccata tatcatcata ggttcctccc ttcgaatatg tgaaaaacac ttcattaacg
     7981 cggcaagcgt tctcgatcag tgaaatgacg tttagtattt cctgaagctt ctgttgaata
     8041 ccagtaagcg acactagcaa catcgtcttg ccgctcaaat aagcgaacat catcgttgcc
     8101 gatttgttgt aaagtgaccg ataaatcttc ggtaaaacag atggggtctt gaagatgcca
     8161 acgataaaga ccatgtcgtg gcattgcgtc gtcaccaaag gcatgaacgg cattcaattt
     8221 tcccgtttca aaacgatctc ttgtggcatc tctatttgat tggtaaggat accctaaaaa
     8281 ggctgttgaa tagtttttcg catgtggacg tccgaactca tcgtactcat gaaaggccca
     8341 cgccccgcca aaataatctt ctgaacctgt tgaggttacg gtcggatact cactatcccc
     8401 atcaatgtag aatttaaact cgccttcgcc ccaccaataa cgttctaggc aggtaagcgc
     8461 aaggtaagta cccacgtaat agccttgacc ctttaatcga tcaattaact tataatcctc
     8521 ctgtaatttg gtaatgcgtt cccgatgcca atttgcgtga aaatataggc tattttcttc
     8581 caattcgtcc accaaagcgt aatttatcgt ataaaaaaat gaggacatat ccttcggatg
     8641 taaattctcg atcgtaattt tagcggatgt ttggaaaggc atttcgaaat aggaattaaa
     8701 gccccctgta ggattaacca ctatcggcaa cgagttcacc tcgcaacgct ctccaaaccc
     8761 attacaaaag aaatccccta aaggactttc aacagcaggg ctatccgacc catcccaata
     8821 aattcttaag attaaatcac gtaaaacaaa actgccaaat tctgttcgat cacgaatcgt
     8881 catccacata tgacgaatta ttcccgttcc agtaatatcc gcaatcacag tttcttcacc
     8941 agaagtcagc ggaattgcgc cacgaccttt gcggcttggt cctagctcgc ttggcaacat
     9001 cgcagcttta cctttctcgc ctgttggatt ttcgggagtg attgctcgac tctggatttt
     9061 tttaaattgg gtaatatttt ttagtatact catctttttc tcctttgaat gtgaatcgtt
     9121 tttccctttt ctacgtgaag aatttttttc gtatcactta ttttcaatgg cttttctgac
     9181 aaatttgaaa tactcaactc ggattgcgta cattcaatcg aaatgaaggc ttgaccgaac
     9241 ttcaaattct tccaagaaaa cgttaaatta tcaacaggat caatttgcaa tactcctttt
     9301 ttgaatttca atccgaaata ttgactgatc attaggctga caaaaagacc cgatgcccag
     9361 ccgcatttgc cgcagccaaa gtctcgcacg acttctcctt gttgagcctc taagcgatac
     9421 ggccaccacc accacgaacc gtctaaatct gccaaacaaa ttagttcttc taatcgggtt
     9481 acgaaagctt gagggttgtg cagctcgctc attaaaacag tgataaagcc aggaaaagtg
     9541 gcgccggatt ctaatcccca agtgatgcct ttgatttcct cgctataggt tggattgaaa
     9601 gaactagcag aaaaggccat ggtgttctga tataaatgat ctttattatc taaaaattga
     9661 taaaaaggca tcaacgtagt gtctgattct tctccatcat gcatcaataa attaattttt
     9721 ccatccacaa tcacatcact taaaaagatt aacccttgct caagaatcgg tttttgatag
     9781 tgcttcactg aataggttgt tttctcctca tcaccgattc cctccaaaaa ttgctcacca
     9841 aaaggaccat cgatagtcat ttgtttacaa attgattcct ttagtttaca ggcaatgttt
     9901 ttgtatcgct tgctaagact cagttgttct agggcttcaa aaatttctgc aagtccaata
     9961 caggccttcc aaagacaaag attgctgccg gtatgatact ttccaagtgc aaaggcgtct
    10021 gataaccaaa cagatggaaa aagcattggt tcccctgacc gacgttgagc taatatgtcc
    10081 tcaataattc ttgaagcatt cagtagaacc tgcggacacc gctcaaagaa ttttgtatcc
    10141 ccagtatgtt caaaataaag actagccagt aacgcactgg ataacgaatt actcagagaa
    10201 tgattgatac ctcctgtaaa ttttgaacca ggaaacttaa cgccatattg atcaaaccat
    10261 agaaccgttt tttgacataa ctccggttca aacatggtga atggcaatga tgcgtaatac
    10321 atatccttat tccaaattcg attagtagca ggatagctgc cccaattgct gccgacgacc
    10381 tgtccctgtc gattcatacc gaaactagaa aagctttgat aaagtgcacg acgatagaga
    10441 tggacaactc tatcgtttgg cattgacagc tgcccaaata gctgagcaaa atagttcaat
    10501 gtttccttca gccatttttc gatatcagat tcctgaaaag tcattacttc ttcaaaagca
    10561 tttgggtcca caaaagcaat ggcgaagctt gccacttcct gttgtgcgat cgtgtcctta
    10621 tttccttttt gagaaatcaa cacattttgc tgatccgaat acttctcttg atacgaaggc
    10681 acatctttca ctgaaacttg aaggctttcg tgtgtttcat tcacaatgta aacctgttga
    10741 acaagcatac ttaaacgctt attagccagt atgggagcaa aggatactac aatgacctta
    10801 aacgtcgaat aatgatgaat atatctaggc aaacagtctt gaatcacatc gacttcaata
    10861 cgatgatccg attctgacaa atggtagaca atcccgttta cttcaacctt taaggataac
    10921 gaattggttt gagagagctg tttattgacc caaacacctg gtttttcctc agttagtaaa
    10981 ttttctcgat aaaaggtcaa cgtttgaatc gtcccgcaaa catcaaggtt tgctaatgtc
    11041 ttttgattac ttacatcgaa ggtcaatccc tttacatcag gaatattccg atagtagctg
    11101 tattcgtttg aagtagtcac atttccgtcg tttaactcag cattaaaaaa tgggtgtttg
    11161 ggtgaataaa ttacttcatt aatagtcatc ttttccctcc ttgctaaaaa aactgcagcc
    11221 tagtagctgc agcttcctac tatgcgattc caagtaatga caataaaatt cccatgacga
    11281 ccattccaaa aataattttg atgacgccga cttttttatc tagtaatttg aaacagctca
    11341 atgttaccaa taacggtaca attcctttaa aaatactatc gaggatttct tggacattca
    11401 ttagttcttc gcccttcatt gaaaagctca aaacggtatt gaatttgacc atactgatcg
    11461 tcattgcacc taccatgatc agacccaaga tactagcagc ttttgtcaga atttcaatta
    11521 acccgttcgc atacagcttt tcaatatatt tagagcctaa tgaatagcct aaaacgccgc
    11581 catagtatcg catcaagtaa ttaggtatat tgaacataac taaaaagata attggtgcca
    11641 aaatatttcc ggcattggct aagttgatcg ctagtcccgc ggtaacgaca cgccaaactc
    11701 cccagaacaa tgaatcgcca attccagcca atggtcccat caaactcgtt ttgatcgcgt
    11761 tgatactttc cacgtcaaaa ccagcttttt cagaattttc cttctccatc gatgccgcaa
    11821 tccccatgac aaaggacgcg caaggaatgg tcgtcgaaaa atactgtgta tgacgttcca
    11881 gcgccgcagc tcgatcttct tcacttttgt agaaatgatt aatgaagggc atcatagaat
    11941 aggcgaaacc accgccgccc atcgtcaccg gattcaccga agcatacata gtcatcgagc
    12001 gccaaaacat cttccgaacc aattttttct catctttaga aattccatca gctaatttca
    12061 ttgaaagaaa tcctcctctt cttggtcaag cgttgcattt acagcagcat cgctttgaac
    12121 tgcatgaaca gatatatttt ttagttgtag atctctttgg gctataacaa ttgcaataat
    12181 cgttccaact gcggcaattg caatcaatgg taactcgaga tacgccataa caacggcccc
    12241 aaagaaaaac cacacagcaa tatttttact ccaaagcatc cgtaacagca tcgccatccc
    12301 aactgccggc agcagatttc cagcgactgt taatccgttc atgatgacct ctggaatatt
    12361 atccaagacg cttttgacaa catctgctcc aaataaaacg gcaaagaatg gaataagagc
    12421 ataaagcgca tagtggacaa accacataaa gaagtgcaga ttgatgattc ctttttgatc
    12481 accagatttt gcaaatttga aaaatttagc agccatcggt ccacatacaa aagaaaaaat
    12541 catattcatt aactgtaatc ctaatactgc tagtggaaag acaatgggaa taatcgcatc
    12601 cacgtttcct cccattttga ttgcgaacgc tgtagccagc ccagcagcca aagaaggctc
    12661 cgccgtacta gagattccga tattgacgac acccataaag atggcttcta gtgacgcccc
    12721 gacaatcaac cccgtctgca aatcccccat caacgctcct gctaacggag caacaacaat
    12781 cggacgactc agcatggaca tcccgaacat ttcatgtcca gcaactccaa taaaaacggc
    12841 caaagcgact acaaatgcat catacataat ttttctcctt cctttttcta aaaatcactc
    12901 aaaatagaga tagctgaacg tttttccatc gccggtgtag gctgaaaaac ggtatctgga
    12961 taaagttcaa ctaattcttt taagtcttga atttctgacg tcgttaacat cacttccttt
    13021 gataaggttt tcttatcttc cgatggcggt gtcattttcc cagcattccc cacgttcatt
    13081 aattcgattt ccgttgggaa agctttcgca acttttaccg catctttgac attagggact
    13141 aaaaccatca agcgcatgtc cttcgcccgc ggatctttta acaaatgaat cgcgtcatca
    13201 actgttttaa taatgacttt gacattcgat ggtgcagcca tcttcaatgt catcttttga
    13261 atatcgttgg tagccgcctc atcgcttgct actactaacc cggttaaatt tagactcttt
    13321 gtccaagtca tcgctacttg tccatgaatt aatcgttcat caattcgcac tgctttaatc
    13381 atttttcttc ctcctaaaaa aaatcttcct cttcatttac tacatgtacc tcatcaagaa
    13441 tggtcatctt taattcctgt tggcatttat ttaccagttc ctgaatatcc tcctttgtta
    13501 attccttggt tgccaatact atttcgagca gaattggcag attaaagcca gacaaaatag
    13561 ttacagcaat ctctttctcc aaaaaggttt tggtaatctt ctgattgaca cttccgccaa
    13621 ataaatcggt aaaaacaaaa atctgatcct gctcgttcac ctggtttaca aatacattca
    13681 actcctgttc aaaatcacac tcatccacat aagcattaat caccgtaatc tcttgaacct
    13741 tccccgtcag taattgaata ctgctaagaa agcctgaagc taagtgaccg tgagtcgcta
    13801 ttagaatttt cttcatatgg ttaattcctt tctttctgtt cacttttata taagcaattc
    13861 ctgtgccaac ttttaaaaac attggtttaa tcacgttaat tcacattaaa aaaagtgtct
    13921 aacctaaaaa aaacagatta gacacttttt tagacacttt atttttgaat atacggtcct
    13981 aataattcat agagcagcga aagctcataa gcattgactt taaaattata tttcatctcc
    14041 attgattgaa aaatacttct agaaacataa taaaattcat cttcatctgg tttcaaatca
    14101 taattcggtt cttcagattt ttctgtccga gccaagaata aacgttccat catcaatgcc
    14161 gtatgcatat agagatttag ttttacacgg ccatttaaag tgaaatgata gtaattttca
    14221 tatttagaaa tgactgtttc cacttcactc atcacgatcg ttgggttcag aaagttcaaa
    14281 cggtccgcaa ttccttccaa cgaaaagaat tttactagct cctgaatcaa gcggttaaat
    14341 tctgccggct tcatttcagg actcaaaata tgctccaaat tggctgctcc atcaccgtct
    14401 aagatgtcat aaacattaat attaggaatt gaaaaggaac gtggaagatc cgaagtcgtg
    14461 atgaccaatt ttgtcttgtc aaaataagtt tcatcatttt ggtcgattaa ttcacgtagt
    14521 tctctgtagt ccttcgtgaa aacttcgatg gattcatccg ataaatgatg acgaaaaaca
    14581 tctttcaatt tgtccgaaat ccccaaaccc gacatacagg aaatgataat gttgctactt
    14641 tgagaaaagc cttcaaagta ttgaacattg attgaatact tgtctgctgc atcctccgca
    14701 atctgtttaa atggcgcatt catcgtcatc ttcaagccaa tatcaagtgc aatggaagtc
    14761 gtcaaattat tcagaagaag taactcccct tttaaatcgt tcttgatctg tgtgtaaatt
    14821 tgatttaaag agcccatatc tacgatcagc actagactat ctgtattcac ttgccgtttc
    14881 acgaaattcc ttgtcgattc gacaatctct gacacattac tctctactgg catatccaac
    14941 gcttcaaaaa cataattgcc gcataattga ttcactactg cttgaatgct gctagccgta
    15001 ctatctccgt gagcaagcaa caggcccttc agctcgatgg attcatctac ataatcagat
    15061 aaagtcacga caataagcag tcttagaaag ttctcgtcct tttctgagag cggtggtaaa
    15121 tgctccaaga actgattaca taagtaagtc gtccgaggta gatttgcagc taatgtacga
    15181 tttatcccgg ttaagtctgt cttatagctt ggggaaatga cataggatct gctaataatt
    15241 tctgcaacag gtccactcgt cgtcaatccg tacgtttttc ctaaaacgga tacccagacc
    15301 tttctaaaca tctgaatcat caattttcta ctcattgata aaaaagaatc cttatcaatg
    15361 tgatgcacca aatgctgtac ttcattcttt atcgaagaaa aattttcatt gaaattacct
    15421 tcatttatca gctgagtaat tttttccaaa atcgaatcat aatcaaacgg tttgattcga
    15481 tccgttttta tcttttgatt tttctttatc aataaactag caatcggcaa gctttctact
    15541 tcggattcca gcatatttaa ctgaatcaag atctcatttt tcccaatcgt taatggttct
    15601 tcttgtgttc gactatacac atccgcacaa ctgattttaa tgatattctt cagcttacca
    15661 atattcccac taaagggatg agataacatt aaagacaagg cttctcttga aacaataata
    15721 tctttttgta aaacatccgc ttcattttga aaaaataatt gaatcagctc taatcgttct
    15781 cgaatggggc gatcttcaaa agttggcaac ataacactaa tttgaatacg ccgatcaaag
    15841 gtgtctaata aataattttc acctttttcc gtcgtggcga aaataaaacg aaccttagca
    15901 gaatgccaat ttacattttc accgactgga cgatagtttc cagtatcaat aaatagaaag
    15961 agcttttctt gattttcttt cgataggcga tggacctcat cgagaaaaag atagccgttg
    16021 ttggccgttg ccaataaacc atcggtatcc ttttcagccc ccgtaaaggc ccccttcttg
    16081 tatccaaaca gtgttgccga aagcagttcg ggattgtccg cataatcggc acagtttaag
    16141 atagcaaatg gtgcatcttt ttcaataatt tgctcatgga tagcgtattc aaaaattttt
    16201 gtggctaaaa aactttttcc aacacccgtg gctccattta ttaatacact gagtccgtta
    16261 ggcggatatt tgacagcggc tatacatttt tgaatgacct ctctcagact tccgtcatga
    16321 ccaactacag aatgaaaggc ttcgtttatt tgctccgtat tatctacaac cgcatattca
    16381 tctaaaggct tccacaaaac aggctttccc ggtaatttct cgaccttttt atcttccact
    16441 aatctcgtca aataatggct ggcattctga cgagagatac ctagattctt cgaaattttc
    16501 tcggcagtga cagaagacat attccctgtt agtcgtatca gctcatcaaa aactcgatct
    16561 ttagcgctct ttttcatttt ttcctccagt ttaatgtgtc taaaagcata ataaactatc
    16621 ggctgcaata tgtctattct atatttgtat tccaattttt actttgaaac acaaatagtc
    16681 gaatatttgc ctcagatatc tgacaaaata atttatctac caatttagtt tttatctaat
    16741 atgattttta tgttttttca tagctacaaa tattgaactg gattcatata ttagttttat
    16801 taatagatta cagaagggtt aagtatattt gcatagtttc caattacttt atccttctgt
    16861 aactttttca atcttagatt tactagcccg tctcgggccc tattttacaa tggttttgtc
    16921 cacttttctc tggtagaaaa acgtggtaga aaaaataaaa taacacaaaa aaagcggcgt
    16981 ttcatccgct tttggtagaa gattggaaaa ctcagataga aaaaggacga tttttagctc
    17041 tttagaacgt tgataagtcc ccattcttac tgagttttgc cattatctcc tatacatctt
    17101 gaattctaag aataggtcat tatagatgcc taggtatttc gatcctagat ctttgtagac
    17161 ttcaagatgg gagatcgtgt catcagatgg atagaattgt tcgtccgacg aaatgtcttt
    17221 tggcaataac gcttttgctt cttcatttgg tgtagaataa ccaatatact ctgcattttg
    17281 tgccgcattt tccggttcta gcataaagtt gatgaaatca taggcacctt ctttattttt
    17341 cacggttttt ggcataacga tattatcaaa ccaaagattg gatccttctg gtgggatcac
    17401 ataatgtaaa tgttcatttt cccacatcat atcggcagct tcgccagaaa atgtcactgc
    17461 cacggaggct tcttcattga tcatatacat tttgatctca tctgccacga ttgctttgac
    17521 atttggtgtt aaatgattca atttatttgc tgcttctgtc agctgtgcca tattcttact
    17581 attcaaagaa tagcctaaac tattcaaaga aagccccata acttctcgtg caccgtcaat
    17641 cagcatcaaa ctgtctttta attccggctt ccacaaatca tcccaatgct taatttgatt
    17701 tgcatcaaag aatttatcgt tatatacgat ccctaaagtt ccccaaaaat atggaatcga
    17761 atactgatta tctggatcaa aatccaagtc taaaaatcgt ggatcaatat tatttaatcc
    17821 tttgatcttc gaatgatcta atggaagtac tagtttttct ttgatcattt tttggatcat
    17881 atattcactt ggtatagcga tgtcgtagtt tgtcccacct tgctggattt ttgtgaacat
    17941 cgcttcatta gaatcaaatg tttcataatt cactttgtat ccatattctt tttcaaattt
    18001 cgtgatcaaa ctaggatcga tataatctcc ccagttatag atggtaagta cctttgctcc
    18061 actcatgcca cttgtttttt caagttgacg aacaccaagc agcagcaccg caataatgat
    18121 gagaatacct aaaaacagag attgtaattt tttcatcgca gccgtgccac cccctcgcgt
    18181 actcttctgt ttttcttcag ccgttttgga cggttttctt tactaataaa gtagtatcca
    18241 atgaccaata tcatggagaa caagaaaact aacgcactta gcgcattgat ttctaaactg
    18301 attccttgtc ttgcacgaga atagatctct actgagagcg tcgtaaagcc atttcctgtg
    18361 acgaaaaatg taaccgcaaa atcatcaaga gaataggtaa atgccataaa atatccagct
    18421 atgattcctg gagaaagata aggcaaaatg atatttttga ctacttggaa attattagca
    18481 ccaaggtctc tagccgcatc gaccattgaa tcgttcattt cctgcaattt tggcaagacc
    18541 atcaagacga cgattggaat gctgaaagca atatgggaaa gcaatacact ctgaaaacct
    18601 aatccgaatc ctagtagtgt aaagaaaatc aagaaactag caccgataat aacatcagga
    18661 gagactaata agatgttatt catgctcaac aacgctgtcc gtgcacgtcg ttttttcgtg
    18721 taatagattc ccatagcccc aaatgtcccg atgatcgtgg cgatcaatgc agataagaaa
    18781 gccagcatga acgtattcaa tacgataatg atcaaacgtg tatcttcaaa aacagcttca
    18841 taattttccc aagtaaatcc tgtaaagctg ttcatcgttc ctccttcatt gaaggaataa
    18901 aaaatcagat agaagatcgg tgcatataag aataaaaaga cgatccctaa ataaagatac
    18961 gaccatttaa attttttcat ttatgagcga cctccttttt tcttctcgcc tgtcaggagc
    19021 atcacgatga acatagcaat gattaaaatc acaccgattg tagaacccat cccccagttt
    19081 tgtgtaacca agaaatgctg ttcgatagcc gttcctaaag tgatcacccg atttccgcca
    19141 atcagacgag tcagcatgaa taaagaaaga gaaggaataa atactgcttg gacaccactt
    19201 ttcacaccat ttaatgataa cggaaaaatc actcgtcgga aggtctcaaa gctattcgcg
    19261 cctaaatctc tactagcact gatcaaagaa ggattcatct cttccaatgc attgaaaatt
    19321 ggcataatca taaatggaat ctcgatatac gttgccacga acagaaaact gaagtctgtg
    19381 aataatatct gtttcggccc gatcccgata aaatcaaaga aatgattcac gctgccgtgc
    19441 acactgaaaa tcccaataaa tgcgtaagct ttaagcagca gattcaccca tgtaggcaaa
    19501 ataatcagca tcagccataa ttgcttgtgc ttcagttttg ttaaaaaata agcagtcggg
    19561 taactgatga tcagcgtgaa aaacgtgatc aaaaatgcat accatacaga attgatcgtc
    19621 atagaaagcg atgtccccga cgtaaaataa gatagataat tattcagcgt gaattgccca
    19681 ttcatatcaa aaaaagattg ataaatgatc attaatacag gcgcgatcac gaataaaccg
    19741 agccacaaaa tataagggac ggaatagatc ctgcgcattg ttttcattct tgtcccccct
    19801 attcttcgta gctgtcaaga cgtgcatcga attcttcttc agactcgttg aaacgcatga
    19861 catggatatc ttctggctca aaggccaatc ctactttgct tccttcttta gcttttttcg
    19921 ttgaatggac catccattca ttttgctgtt catcataaca gatgatttca taatggacac
    19981 cacgaaacag ctgagtatcg accgttacga ctagttttcc tttatctggt gtcgtgatcg
    20041 tcaaatcctc tgggcgtaaa acaacttcaa ctggttcatt tagacgcatc ccgccatcga
    20101 cacattcaaa ttgcttacca acaaactcaa ctagattatc ttcgatcatt acaccgtcca
    20161 cgatattgct ctccccaaca aaatcagcaa cgaaatgatt gattggctca tcataaatat
    20221 ctacgggtgt accactttgg acgattttcc ctttattcat gacaaaaatc tcatcactca
    20281 ttgccaacgc ttcttcttgg tcatgagtaa caaaaataaa cgtgattcct aatcgttgtt
    20341 gcagatcccg cagttcatac tgcatatctg tacgtagttt caaatcaagc gccgataacg
    20401 gttcatctaa tagcaatact ttcggttcat ttacaattgc tcgtgcaatc gctacacgct
    20461 gtctttgacc accagacatt tcactaatct cccgtttttc gtatccagga agctggacca
    20521 tacgcaaagc atcttttacc ttcttttcga tatctgattt ggacattttc ttgatcttca
    20581 atccaaaagc aacgttatca aaaacattca tatgaggaaa aagtgcataa tcttggaaga
    20641 ctgtatttac ttgtcgctta ttagctggca aatcattcaa ccgtttccct tcaaaataga
    20701 tgtccccttc tgtagcttcg gtgaatccag ctataatacg taggatcgtt gttttaccac
    20761 agccggaagg accaagcagt gtataaaatt ttccttgttc gatctcaaaa cttacttttt
    20821 ttaatactgt ttcatcatca tagcgtttca ctacttgatc aaatgaaatg attggtttcc
    20881 ccacgttttt accccctata ttacaaatat gattgcgttg caacaatcaa tacttttgtt
    20941 cgttttttga gatgattcct taattgatgt tgttttgtcg cttgataata gatcgactgt
    21001 cctgcttttg ccacatagac cgtctctcct aagcttaact ctaattcccc ttctaataca
    21061 aaaataaacg tttcagataa tgaaggttca aacgtcttat attctcctgc accttcaaaa
    21121 gtcagcaaga caggctccat ttctttttca ttcgaatcag ccacgagcca ttccaattca
    21181 tacccgtttt cttcatcgaa ataaaccgta tgatcttctt ttgaataaac cacttgcaga
    21241 ttcgctgctt cttgatgaaa aaattcttct ggagtcacac ctaagacttc aagaatagaa
    21301 aagaatgttt ccattgatgg tgaacttaaa tctcgttcca actgggaaat ataccctttt
    21361 gacaaatctg tgcgttcgcc tagttcttct tgtgtcaaat tcttttggat gcgcaaattt
    21421 cttaacttct caccaatttc cataatttcc tccgaatcga agttttctaa tagtaaactt
    21481 cctgtttact accactcgta ttagtatacg aaatttattg aaaaaggcaa ccctttgtaa
    21541 aatttttttt gctgaatatt tctttatctt ttggaataaa ttctagttgt ttgattcttt
    21601 atttttcacc taatttttgt ctaaatttac ggaagaacaa gaataaaaga gtaggcttta
    21661 tggtatgata aaagaaaatt ttaaaaggag tgtctcacga atgaacgctt ttcgatcttt
    21721 tcaggtaata aaggaacctg ttttccatac agcaatcagt gatctgccat ttactgagtt
    21781 ggctgaaaat gaagtcttgg taaaaattgc ctattctgat gtcaattata aagatgcatt
    21841 ggcaatgagc gagtctggtc aggtgatacg aacatatccg atgactccag ggattgattt
    21901 atccggaact gtcgtccaaa gtaacaatcc acgtttttca aaagaagatc ttgtacttgc
    21961 tactggtttt gggcttggcg tcacccatcc aggcggttat agccaatatc aaaaagtacc
    22021 aggagactgg ttagttcctc taccaaaaaa tatgacgctg cgtcaagcga tgatccttgg
    22081 tacagcaggg tttactgcta tgttgtgtgt gaacgcttta atccgtcaag gtatgacctc
    22141 cgataaaaaa gttgtcgtta ctggtgcttc tggcggtgta ggcagtacag cgatagccct
    22201 gttacataaa ctaggattcc agtcaatcat tgccttttcc agaaaagaag agtctgtcac
    22261 atggctaaaa agtctaggtg ctttgcaagt cgtacgacct gatgaatttc tgccagagac
    22321 aacaaaagct ttaggaaaac agcaaatcga ctatgtcatc gacacagtag gtggcgagca
    22381 gctaagcagt cttcttccat tgatctctta taacggtgca gtagctttat gtggcaatgc
    22441 tgggggaatc aaactaaacg cgactgtctt gccttttatt ctgcgaaaca tccagttgat
    22501 tggaattgat tctgtcaatg taccaataga tcaacgtctt tctttatggc aacagatggc
    22561 ggatttgcag attgcagatg aactggtcgt ccaagagatt actctggatc aattgcctga
    22621 aacagcatct aaactacttg ctggaacaca ccaaggaaga acactagtaa atgtaggtga
    22681 ccataaatga agattctaat cacagctgga ggaacgactg aaaaaatcga tcaagtacgt
    22741 gctattacaa atcattcgac aggacgatta gggcaagcat tagctgatta tttagctgcc
    22801 aatccggata ccacaatcga ttacgtgact acgaaacaag cactgaaacc gaaaagacac
    22861 tccaatataa cgatttatac gatcgaatct gcactggact tatttttaca attagaagaa
    22921 ctaacgaaaa aagaacacta cgatgcgatt atccacagta tggcagtcag tgattttact
    22981 cctgctttta gtttttcaga agaacagctg gcaaaaaacc tacccacttc ttcaacgcaa
    23041 gaagaactgg acaattggtt tgcggaaaat gaacagacag ataccactgt ttcaaagatc
    23101 tcttctaaca ccgagcatct tgtgctcgtt ttgaagaaaa caccaaaaat catttcttat
    23161 ctacgtgaat ggcagccaaa agcaaaaatc attggtttca aactattagt agatgtacca
    23221 aaggaatctc ttctggcagt cgcaaaaaat agtctgataa ataacaaaac tgattttatt
    23281 ttcgcaaatg atttgacgga aatccatggc gagacacacc acggctatct cttatctaaa
    23341 gatggaacag tggaagaagc tcaaagtaaa tctgagattg ctgcattgat tactgagaaa
    23401 attagactgg aggaatcaaa atgaaaaaga tcttactagg cgttactgga agtatttctg
    23461 cttacaaaag cgctgatctt acaagtcaat tcgtcaaatt aggtgcccag gtagatgtga
    23521 ttatgtctaa aagcagcaca cattttatca cgccattaac cttgcaatct ctgtcgaagc
    23581 gcgcagtcca tacagacgtc atgcaagaat caaatccgca ggtaatcaat catatcgaac
    23641 tagcaaaaca agctgacttg tttctcatcg cgcctgctac agcaaacatt atcggcaagc
    23701 tagcaaacgg cattgcagac gatcttcttt caacagtcgc aatggcgctt ccagtgggaa
    23761 caccaaaatt gattgcccct gccatgaaca cgaatatgta tcaaaacccg atcactcagc
    23821 gtaatctgga aacattgaga tctgtcggct atcaggaaat tgaaccaaga gaatctcttt
    23881 tagcttgtgg agattttgga aaaggtgcct tagctgatat tcagacgatc atcgaacagg
    23941 tcatgctgac tcttaatgaa agaaatgctt agaaaaaagg agtacttatg aaaaatacaa
    24001 aaaactttac attaactgcg atgtttttag ctattttgat tttattagct gttacgcctt
    24061 taggttttat ccctatcggt ccaatcaatg caacaaccat gcatattcct gtaatcattg
    24121 cttcgatcgt tttaggacca agactaggtg cttttcttgg cggtacattt ggactgatca
    24181 gcatgatccg tagcacagtt atccaaacac ctttgtcttt tgtcttttct ccgtttatcc
    24241 cagtcatcgg aacagaacat ggaagctgga aagccttgct gatcgcgttt attcctcgca
    24301 tcttgattgg ggtggttcct tatttcgttt ataaaggtct gcgcaaacta atgaaacaaa
    24361 aagcagattc cgtttcttta ttcttagctg gactttccgg ttcattgacc aatacgattt
    24421 tagtgatgaa tatgatctac tttttattcc agcaagatta tgccaaagtc atcggaacaa
    24481 atttaaatgc tgtttattca gctattctag cggttatttt tacaagtggt atacctgaag
    24541 gaatcgtcgc aggtcttgcg actgcagcag tatgcaatgt cttgttaaga tacttcaagc
    24601 acccttctct tactaactga tagtcatcgt agccgatctc tgattttttt ctcttttggt
    24661 gttactattg aatggaaaga tttgacgtcg aaaggatgat ccaaatgaaa atttttgttg
    24721 taggtgcaaa cggacaaatt ggccgacacc tgataaaaga tttagctcca agttcccatg
    24781 aaatttttgc aggggtgaga gatgttgctt cccaaacatt agtaaaaaaa gagaatgttt
    24841 cgtatgtctc ttttgattta acttggtccg tggaaaaaat gaccgaagca ttcaaaggca
    24901 tcgatgtatt gatttttgct gccggctctc aaggaaagaa tcttctgcaa gtagacttgg
    24961 atggcgcaat caaaacagtg atcgcagctg aaaatgcgca tgtctctcgc tatctgatgg
    25021 tcagtgctgt ttatgcagat gaaccagcaa aatggcctga gtccatgaca gattattata
    25081 ttactaaaca ttatgcagat gaatggctga aacgaactaa cttagatttt gtcattcttc
    25141 aaccagtcac attgactaac gatgaagaag ttacttccat tcaattaact aagcctaatg
    25201 agaaggcatc aaaaacgatc actcgctcta cagttgctgc tgtacttgcc gctctagtgg
    25261 aagaaacgga tatttctcgt acgactctag tactttctga aggtagcaaa gagttgaata
    25321 cagcattcca agaatgggca aaggaggaat agatccatgc gtgccatttc aatcaagcat
    25381 cctggttctg tagatgtatt agaacaaatt gaagtgcctg ttcctaaacc aaaacaaggc
    25441 gaattactga tcaaagtcca tacagcagct gtcaatcgaa cagatattat gaaaagagaa
    25501 tcagtctctt tgaaacctcc ctaccctatt ttgggagtcg aagtagctgg agaggtagtt
    25561 gagaataaaa gtgatatctc cacttttact cctgggacaa aagtatacgg attagtcaat
    25621 ctaggtggtt atgccgaata tgctgtgatg cctgccgatc gtgcaatcct tctccctgat
    25681 acgctagact atgtttctgc tgctggaatc tcagaagttt ttttgactgc ttatcaaaca
    25741 ctttactggc ttggaaaatt acaagaaggc gaaactgtct tgatccatgc aggagcaagt
    25801 ggtgtgggaa cagcagcgat ccaattagct agacagcttt ctcacgcgaa aatcatcgtt
    25861 actgcaggat cagaagaaaa actcgccttt tgtaaagaac ttggtgcaga tgaattgatc
    25921 aactataaaa aacaagactt tcaagaagag gtacgaaaaa tcactgaagg tcggggagtc
    25981 gatgttattt tggatttcat cggcgcttct tactgggaaa agaatttagc ttctatagcc
    26041 gttgatggac gttgggtctt gattggtatg ctaggtggga ccatcgtgcc tgagatagac
    26101 cttggtgtat tgattgggaa acgtgtccaa ctgcttggta cattattgac acctagaagt
    26161 gatcagtata aagcagagct cgcacaaact tttatgcaaa aagctgggcc ttttctagaa
    26221 aatggcaaga ttcggccaat cattgaccgt acattccaat tcagcgcagt caaacaagcg
    26281 catgaatata tggaagcaaa caaaaacatc ggaaaaatca ttttgaagat aactgaataa
    26341 aatcatgctg atcaaaaaag aggctgggac agaagcgttt aactccaaga aataagaaga
    26401 aattcacgaa aattgctttt caaatttttg tgaatttcag cttatttccg aaggaatttc
    26461 ttctgcttcc gccgtttata cgtgtttaga gcgtgagaca aaagtgttct ttacttttgt
    26521 cccacgctct ttttccgaat aaacggtgtt caaaagcttg catctgcggt aataagtcca
    26581 accaagttaa aaacataaga agctgttttt acttggctgt tcttattact tgatgcaggg
    26641 cagctttttc acaacctctt cactaaacag tgttcaatca gctgaccctc ggtattagct
    26701 caaaacgctg atttcacaac ctctttttat ttacgttcta tttcattcat tttccgaagc
    26761 aaatcgagct gcttgtatgc ccgcttgtcg cccaaaaata atgatctccg caactgaatt
    26821 tccaccgatc cggttgtttc catgtaagcc acctgtgact tcaccagcag catataaccc
    26881 cctaattggc tggttttctt tatctaatac ttcagtgttc gtattgattt tcacaccgcc
    26941 catcgtgtaa tggattcctg gtgcgatttt gatcgcataa taattcggag acgataagtc
    27001 atgttccatg gctgtggcac ggttgaattg agcatctttt ttctccgcaa catccgtatt
    27061 ccatccatca actgtttttg ctagtgtttc ttttggtaaa tcgatttttt ctgctaattc
    27121 ttccattgtc tttccagttg tgacatatcc tttttggtca taaaattcaa tagctgtggc
    27181 acgatcacgc acgccttggt caaaaatcaa ataagctgat ttttcaggca atgctgtaat
    27241 tgctgcagat actttatccc ttgtatccat ttcattgaca aatcttttcc cctcttgatc
    27301 gaccaagatt gctccttctc cgcgaacaac ctcaccaatc aagaccccct catcctgctg
    27361 cacagttgga tgaatttgta ttttatccat atcaacggtt tgtccaccta atttttcaat
    27421 catggtgata ccatctccag ttgatccttc ttgatttgtc gtaatataat cagctaaatc
    27481 gggacgatat ttttcaataa attctttgct ggcaccaaaa ccgcctgtgg taactacgat
    27541 tgcttttccg gtgacctctt ttggttcttc tccttgaaca tgtacagtca cttcattgac
    27601 cgtttcccca tttttcttaa tgtctgtcac atctgcgttg acaaaaatag ggatttcccg
    27661 ctcatggacg ttattcaata acccctccac taagtatcca ccaatagccg agccatcaga
    27721 cggacgatgt gtcctctttt cactcatccc tccagtgatc gttaagttgt ccagtttgat
    27781 accgttttga tctagccaat cgaccgcatc agcagagtga tcgacaaagt atcgaagcat
    27841 ttctttatca ttcgttcctc caccaccttt tagcgtttct tcatagaagg catcattcga
    27901 gtctttgatt ccttcttctt tttgaaattt tgtttctgaa gcattcatac cgcttgatga
    27961 tttagatgtg tttcctccgg caacaggcat cttttccaaa attactggat tcaacccagc
    28021 atcttttgct tcgatagctg ctgccatccc tgcaccacca gctccaataa tcacgacatc
    28081 ataagattct ttcatttctt ttggatctgt atatacttgt tcagaagcgc ctgatgatac
    28141 ttcggcagat tccttcgtac tagaacttgt ctgcgctgta tttttctgat ctgcgccgca
    28201 tgcgctcata gcaagaagtg caacggctgt taataaacca gcaactaatt ttttcatttg
    28261 tatccccctt ttctccttat actatattaa gtgatttttt tcacaatatc aactctgttt
    28321 ttctttaagt catatagctt tggtaaacac acgcttctta gtataattaa ggatatacat
    28381 gaaggagtta ttttatgaat catttgaaaa ccttttttac gaacagtaca aaaactgcag
    28441 gtagaatcac aaacggaatc tatattttat tacgtatcat cgtcgttatt atgctagtgg
    28501 atcaggtctg gaaccaacac tggggaaata caatgctttt attactgaca ttaattttat
    28561 ttgaaatccc ttattttctg gaaaagatat taaagataca gattccaaat atgctagaaa
    28621 tcattttgtt atcttttatt tttagttcaa cagttttagg agagttaagt gatttttatg
    28681 gctatttttc aatgtgggat acttttctac atgcattcag cggattttta gctgggggtg
    28741 tcggcttttc ccttgtttat ttgctcaata aaaatacaaa aaccatgaac ttgactcctt
    28801 tattactagc agttgtttcc ttctgttttt cgatgacagt tggtgttatg tgggagtttg
    28861 tggaattttc agcagataaa ttactgcaat tcgatatgca aaaagatacg atcatccagc
    28921 aaatcaatag tgtggagtta agtaaagacg gaaacactgt ctatcatgtt ccagatatca
    28981 ctcggacaat cattgaaagt caaaatgcag atggtaaaac agataaaaca atcatccaaa
    29041 atggttactt agacgtggga atcatggata ctatgaagga tttatttgtt aattttattg
    29101 gagcagttgt tttcagtgta tttggttacc tctatagtcg aaacgattac caaaaattta
    29161 cgttcatcaa gaattttatt cctaaaaaaa tcaaacgata aaacatgcta aaaaacaaga
    29221 gaaggggcag tgacaatact tttgtcactg ccccttcttt tttgaatgta tggcatgcca
    29281 ttagttatcc gatagtatta actcaaaaaa gtaagatatg aggaatgact atttcgtttt
    29341 ttatcccaat actgaggact tatttcacca tttcgttatt tatctaattt acttaaaacc
    29401 tgcataactt tttcataatc tggattaggt gtgccgtcta gattttcagc aataaaagta
    29461 aaacccattt ttttataaag agcaatcgcc tgatggctcc atgtctgtgt gtgaagatag
    29521 attggtccgt ccgtatggag ggtaggaaat tcttgcaaga ctctggcagt caatgctttt
    29581 gcgagccctt ttccttggta atctggtttc actgctaacc aatgaaaaac cggatacact
    29641 tgctctcttg atttttttcg ccaagcggta caggttgcta ctttttctcc tttttttgtc
    29701 tctacaaaaa acatccttct ttcaagttct tcaggataag gagcaaagtt ttgttgaaaa
    29761 tactcaagtg cttcatctgt cgtttgaaat tccccaacgg aagtctcaat ctccgcccaa
    29821 gcagattcat cttctttttg ataaaatcga aattcaaaac cttccgctaa aggcgtgatt
    29881 ggaacatttt gttgaagtgg tcttgtcatc cagatttctg cgtaaggtaa cgttttatct
    29941 aacatttttc tatccctcct cgtctccatt atgccaaata acatatgact cagctactta
    30001 tttgactata acagatagag atataaatca gaaccagtca tctgcgtcaa attcatttcg
    30061 attagcgtat acccatgttc ctgccatacc tcgcatgaaa tctcctaaag cagaagaagg
    30121 aatacctcca ccaggtgcgg gtgttcccgg actaggaagt ggtacttgcg gctgcagatg
    30181 ctcatcggtg gaatcaatag ttgacaagcg ttttttcttc gtttccttct ggtcatctga
    30241 ttccttctga tcttccgact gtttagcaac aaattctttt tctgattgtt gttttatttt
    30301 aggctctaaa atttcatttt ccaatccaga aacttgtggg gccaaatgag cttcttgcct
    30361 tatcgtttta ttagattctg agatttttct agatattctt gctaactcta ggcttcttgt
    30421 ttgttttact ttccctgttg gagaaactgc atgagccatt tgttgttttt tttctgacaa
    30481 gctttcttct tgtctttttt gttctttttg atctatctgt ttgtttaatg gtaaaacatg
    30541 taatagtgac tgagcatgga gttcttggag tgaatacggg tcatgataac tatacataat
    30601 cgtaaaatca cctgtttgtt tggtcaaata accatcctcc aatggtactt cattgattgt
    30661 aaccgataca tcctttaacg gtacgggttt acctaaggaa tttgttgctg attggatcaa
    30721 ctcttctttg tttatttttt cgtttggatg aacaataaga tcatgacctt ctaatgaacg
    30781 attgtctggt atataaacat aagtaaaggt aatttcccct cctgattgct tcctattatt
    30841 catcagttct gagtgaagta cttcaaggat ctccgaccag tgactttttc tgtctaatcc
    30901 gtttgcttgc atccaatcag aaatagaagt gactttccca ttttgttcat aagtcgataa
    30961 atctatattt ggcactcgat cagaaaattc agcaggtagg cgcaaatcag gaatacgatt
    31021 tacactactc atatgtgttt ctgcttgaag ttctttattt gtttgactat ctataaaatg
    31081 agtgatgaca gtcgatgaag cagcagatgc ttctacataa actacagctg ttgtttctgc
    31141 cacatttgat gttttcgagt ctatataagt taatacaacg ggatattctc ccggagttgt
    31201 agtatctact gtcccgttca ttttgatttc ttctttattt cctgaagttc catcgctttt
    31261 tgttacatat tcaacaagat ctttaggggc gtaactctct cctgttctta agtgaagatc
    31321 ttttcctttg atagctgttt gatcagcacg aacatggatc acagcagatg cagtcattgt
    31381 ttttaaagca attggatcaa aataggacaa ggaaatagta gatgttcctg tattagtcgt
    31441 atccacgata ttcccattgt ttgaaattac tttggacata tctccttgtg ttccatctgc
    31501 gttcttcagc ctttctacca gatcagatat cgaaactttt gtccctgcca gaacatcgat
    31561 tttctttctt tcaaactgtg aataatcttt ttcatataca taagaaactt gtgcgcttcc
    31621 accgtccttt atatctgtca aagcatgatt caaaatatca gtgatctgtt ttaactgatc
    31681 accttttgta ggatcagtca tatcggaaaa gatagaaagc ggcacactgc ttccggtttc
    31741 cgatgtccat aatgttttgt taggaattaa ccagtagtgg tttatcgttg gaggataact
    31801 gactttatta taggaagtca atgcaggaag tagctgtttt ccatttgcat cagtgaaagt
    31861 atgagtaata gtgatagggg aatcagtggt ctcagaaaca gaggtacgta cgaccttgat
    31921 ggtttgtgtc gttgcctgaa cgggtacatg catatagaca cctacagata aactgatcag
    31981 catcgtgcgc atcataaact taacgtagtg tttttcagaa cacgaccgtt ccttatctaa
    32041 acgacaagcg tgtttattta gttgttttga acgtcttttt gaacacattt attcttagtc
    32101 ctccctactc ttgtctaaac atgaagtagt aattttcata agatgatttc attatataaa
    32161 taaaatcaaa agaagatgga ctattaggag agtatttttt ttgatttttt attgtttcaa
    32221 gaaaatcgga ataataactt atgtactttt taattttgtt aagtaatata tctagttata
    32281 tctagttata tctagttacc ttttctttga aaaaaacata aatcagctaa taaatatgtg
    32341 agacaaagct gccatccata aatactagct gattttttca gttaaatgtc tatttttttc
    32401 tctgatgatg gtatttccaa aatattccca gtcatctgga gtcggtggat ttttccaaat
    32461 tatctgttct ttattaaatt tttcaagtaa acaatcagat aaataaatat tcgtacgaga
    32521 agtgattcga ctctcaatca caatattgta atttttaaat cctttcactt gagaaataat
    32581 gtaatcggta taagcagatc cttgcgaaaa atcaatacag acatgaatcg gtgcttcaaa
    32641 atatgatacc ggacaaatac cgaccaataa aaagagataa tcataaaata agcgaatcat
    32701 gaaacgttca tcttttccca agaaatagcg tttattctct tgtatgacct tttggattgc
    32761 tgtactgaat attggatacg tctcgaagaa aaactgtagc tgctttttag acgtaaagct
    32821 agtcatctca aagtcgaata tccaattttt tcgattgaca tgcatcatac ccatacgaag
    32881 ttcttttcgt atatatagtc tttcttcttc agagatttca atcgaaaatg gtaattgatc
    32941 aatcaattcc actgacattt tttgactaat catgtggata tcatgaaaat gagcttccgc
    33001 aagtccaaat gcgccatcta attcaaattc ttcttccgtg atcaaaaaca acatgatata
    33061 agaaatctca tcaatgatct gctctttagg gatatgaaga aaagaaatat gtgataaatc
    33121 cttgatgatc ttcattcctt ctgagctctt aaagtattca tctgaaaaga aacgttttcc
    33181 gattggttga ttcccatagg ccctcaacaa actaatagtg gttagcaaat tgaatttgat
    33241 tcgttttgtt gcagacagtt ttaaattgaa cagatattca aaatattgca cgatactctt
    33301 tgcttcattc atgacgcttt ctctaaatgg catagaaaaa ccattaaaga ccccgaaata
    33361 taccccaaat aatacgttcc gtatgtttat ttcttcccca actaatatgt ttttctttaa
    33421 ctttatttga tgctgttcaa gaaaagagac taattctctt ctttttacat aggctcttga
    33481 tatgcttaaa aaatgattct ctgcaaattt ttcgatcgtt acttttgaat ccacgatctc
    33541 cgttaacagt cgagcaattt ttgatcgttg aaagtagatt cttcttaaat ttttgatatc
    33601 cgtcaaattc agacgttcga catggatctc tgcttcttct gaaatttgga tctctggctt
    33661 tgttttgaat tcagtaaatt ctttatttaa ttctatgata taatttcttg ttttaaattt
    33721 agacaaacct aatgtttcat ataacttagg cagcactaca taattctttt tctcaaattc
    33781 taaatatcta gctagcagca gtttttctct atcttcctct tctataaggt aatccaactt
    33841 catatcctac acctcaaaaa atgcctcata ccaattactt gatatgcaga tgaaagttca
    33901 tcttttcacc tatactgatt ttatgatgga ttatgcttgt tttttcatca gttattctca
    33961 ttttttatct tttttttgag aatattgcaa aaaatggaaa aatttatgag gaaatattct
    34021 atattttttg ttttattgtg ttaaaatgta caagatttgc gccatttgcg taaacaacca
    34081 caaaaaagag caggacggcc accgtcctgc tcttttactt attttaagtt ccattactta
    34141 ttaattggct aaacttctgc ttgtttctga tattgcttgt ttttctccaa tgatccgcac
    34201 ttctgtttct aaagacacat caaatttttc tttgattact tcttggatgt gcgcaatcaa
    34261 ttcaatataa tctgtcgctg tcgcatgatc gatattgacg atgaatccag catgtttttc
    34321 agagacttgt gcaccgcccc atttcaggcc ttgcaatcct gcttgttgga tcagctgacc
    34381 tgtatagtgt cctactgggc gcttaaagac acttccgcat gaagggtatt ctaatggctg
    34441 ttttgattga cgcaattcgg tcaactcatc cattcttgct ttgattgctt cataatcccc
    34501 tgtttgcaaa ctgaatcgag cttccaatac gatagcacgt aattcttgga ctttactgtg
    34561 acgataagaa aataccattt cgtcttttgt caaagttttc aatgttccat cttcaagcaa
    34621 aagatccact tcagcaaaaa catctttgat ctctccgcca tacgcacctg cattcatata
    34681 aaccgctcca ccgacacttc ccgggatgcc gcaagcaaat tcaaatccag tcaatgattc
    34741 tgccaaagct gcataagtcg tatcaatcag tgaggcacct gcttccgcaa tgaccatcgt
    34801 tcctttgaca tgaatctgtt tcatttcagt cagcatgatg actacaccgc gaatcccgcc
    34861 atcttgaacg atcagattgc ttgcatttcc taataccatc caaggtatat catggattcg
    34921 gcaataatcg atgatttgct ttacttcttc ttgttttttc gggaatgcaa ggacatcagc
    34981 tggtccacct gttttcgtaa acgtatagtt catcaatggt tcatcaaaca atatagtaat
    35041 cgtggctaag ctttcaacta tttcttcttt attcactgag gattttcctt tcttttctca
    35101 aacgtcctca ttattctatc acgcttaata caatcttgcc agtcttgttc aaagttgatg
    35161 acggtagtga tctttaagca cttcctcaca taattcatga cttcttacaa cttctttttg
    35221 tttaagcaat tctgtttccg atttacctaa acggatctga gaaaagaagg cctctaccat
    35281 tggctcaaac ccgcgtttga ctagcgtgtt tgtccagtcg ccaaattctt tcactttttt
    35341 tccatcagca tcttgaatga caagctgggt cagatcgttt acttcataag taccattttt
    35401 acttgttact tgatattttt ctatattagc gccacttgct aaatccatcg ttaaaatagc
    35461 cgtttgattt tgtgtttcta attgtaaaat ggcatattcc aaaaattcct ttgtttctct
    35521 tatctggtat tttgttctca aaatgggttc atctaataaa tacacagctg tatcaataag
    35581 atgtaaaaac aaatcataaa ggacgaactc tgtcgtttct tttgccgcta ttcgattttt
    35641 ttgcaggtgg atcaaccgct tatctgggat acgtttcaat tcttctacca taggagcaaa
    35701 gcgccggttg aaaccgatca tcaaaattat ttggttctta tcagctaatt cttgcaattc
    35761 acgaacttca tttagatgga cactcagtgg tttgtcaata taaacatgga taccattttc
    35821 tagacatttt ttggctaatt cataatgtac ttttgttgct gcgtggatca tacatgcttc
    35881 gattttttct tctaaaagct catcgatcgt ttcatataga tgttggaacc cgtactcttt
    35941 ttgaattctt tcttttgttt ctttatttct tgttgcaaaa taaaaatcaa gtgttccttg
    36001 ttttttcgca taagttggta aatatgcttt ttctgcgata ttccctacac cgattacacc
    36061 aatcttcatc tactttcctc ctggatcatt tagttacttt catcttacac cattttttac
    36121 taaaatgagg tacaatagta tcgcaaaggt aaggaaatta attcgaagga gtgtgctttt
    36181 attgaaattt atctcttgga acgtaaacgg gcttcgtgcc atcgtaaata aaaatttttt
    36241 agaagttttt catgagttag acgccgattt tttctgtctg caggaaacga aactccaagc
    36301 aggacaaatt gatttagatc ttccaggtta ttatcaatat tggaattacg cggaacgaaa
    36361 aggctattcc ggcacagcga tttttgctaa aaaaccagca ttgaatgcca cttacggaat
    36421 gggtatcgat atccatgata cagaaggccg tctgattact ttggaatatt ccgatttctt
    36481 tcttgtaacc tgttacacgc caaattctca aagcgaatta aaacgtttag attaccgatt
    36541 agaatgggaa gaagcctttt ataattattt ggaaaatcta aaaaaacaaa aaccagtcat
    36601 cgtgtgtggg gatctaaatg tcgctcatca aaagattgat ttgaaaaact ggaaaacgaa
    36661 tcaaaaaaat gctggattca ctccagaaga aagagcggct ctttctcgac tgttggataa
    36721 cgggtttatc gatacattcc gttattttta tccaacccag gaaggtgtct actcttggtg
    36781 gaactaccga ttcaattcaa ggaaaaacaa tgctggttgg cggatcgatt actttttgac
    36841 gagcaaagat ttagagccag gactagctga tgcaaagatc catacggaca ttatgggcag
    36901 tgatcattgt ccagtagaat tagatttgaa ataaagaggt tgtgaaaaag tcgtttagtt
    36961 tcgagtatta agaaaaattt tgtgaaaatg gctttccaca ttttgcataa atttggcata
    37021 ataccgagaa actggctttt gaacactgtt tatcgagaga ttgtgaaaaa gctgtcctgc
    37081 atcaagtaat aagaacagcc aaacaaaaat agttcctcat atttttcgtt tggttgggct
    37141 tattaccgca gatacaagct tttgaacacc gtttattaag aggttgtgaa aaagctgcac
    37201 tgcatcaagt aataagaaca gccaaacaaa aata
//
LOCUS       DASHHB010000026        36406 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.026, whole genome shotgun sequence.
ACCESSION   DASHHB010000026 DASHHB010000000
VERSION     DASHHB010000026.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 36406)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 36406)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..36406
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.026"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            136..309
                     /locus_tag="U2K71_001417"
     CDS             136..309
                     /locus_tag="U2K71_001417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315506.1"
                     /translation="MMKRLYYSLIITIGYLIVSNLGNMVFGISKEFSWTTTLWESLFF
                     FIFVFLLQNYRKK"
     gene            949..1422
                     /locus_tag="U2K71_001418"
     CDS             949..1422
                     /locus_tag="U2K71_001418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2975 domain-containing protein"
                     /protein_id="HEM7315507.1"
                     /translation="MKFKTLLLKFVVLGISAVFILFGVLFYTQIATSEKGFNFDWQTT
                     LFVSVIYFALLLGIVIAYFLLKLLKKIEDNQAFSTQSLNILKKIERTILFIGFAFFGL
                     LPKFMKVADLDDAPGLMLVGIAVVFIPFAVYTLTSVLEKLLEHAILLKTDHDLTV"
     gene            1431..1658
                     /locus_tag="U2K71_001419"
     CDS             1431..1658
                     /locus_tag="U2K71_001419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315508.1"
                     /translation="MAIIVNLDVVLAQKKMSVTELSEKVGITMANISILKNGKAKAIR
                     FSTLEKICQVLECQPGDLLTYKNDSNTIPEE"
     gene            2294..2665
                     /locus_tag="U2K71_001420"
     CDS             2294..2665
                     /locus_tag="U2K71_001420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315509.1"
                     /translation="MPFIVYFFLSLLVIYIPLPTFFLLVNRLAEQHDTIAALTKLCLL
                     LFVCLDYFTSYYWLKQTRVKKRYYFFLVFFNLFEFLIHCFLLHQYHTIHLWIIWVFKV
                     VVLFCMFTFPISRKFREALFY"
     gene            complement(2921..3163)
                     /locus_tag="U2K71_001421"
     CDS             complement(2921..3163)
                     /locus_tag="U2K71_001421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM7315510.1"
                     /translation="MGFIWSLIVGGIIGAIAGAITNKGSSMGIIANVVAGLVGSALGQ
                     ALLGSWGPSIAGMAIVPSLIGAIILVLIVSFFARKL"
     gene            complement(3195..4097)
                     /locus_tag="U2K71_001422"
     CDS             complement(3195..4097)
                     /locus_tag="U2K71_001422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM7315511.1"
                     /translation="MNNNNKNNNNKMNNQKPVSAHPDPTTPNQSNGVNDEKNKTAPVS
                     AHPDPVVPNVQQGVEEKKHDAEKHAPHHPGTPESKVPEQHDAIDPSKPVEEQKHPSDH
                     AKAQEKLSSDHTNGQGKLGSDHTNNQGKHDSEQEDSMRGELNYEDKVVQKIIGIAIEQ
                     VDGLLSANGGFFSNVAGKLVNTDNVTAGIETEVGKKQVAVDMDVIVEYGKDIEKIFEE
                     MQEVIGKEVKNMTHLEVIEVNANVVDIKTKEEFEKDQETVQDKVTDAAKKTGEFASNQ
                     TDKVKSAAGSGAQKVKENTEPRVQ"
     gene            complement(4110..4298)
                     /locus_tag="U2K71_001423"
     CDS             complement(4110..4298)
                     /locus_tag="U2K71_001423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEM7315512.1"
                     /translation="MKELFSTYKLPIIFGVLGLILAVLWITVGFFKTLLILIMAAIGV
                     AIGFYLKQTKILDHYFKS"
     gene            complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="U2K71_001424"
     CDS             complement(4312..4875)
                     /gene="amaP"
                     /locus_tag="U2K71_001424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEM7315513.1"
                     /translation="MNKGVKTIGVIGTLLLLSVLLFAILLSATYPLPFQLEKFRFFSI
                     TNSYVQQYVFWLAVAFAILTIITLLVFLFYPKTIGNFLLKHGDGELTLDKKAIEGMVR
                     SHLHEDEFIHSPKVNVKATKNKIAVNVKGELKRTSALIGKTGTLMKEIENEVQHILGT
                     KEPVKVSVKYTGFEEPAKRSNDRKRVE"
     gene            complement(4913..5806)
                     /locus_tag="U2K71_001425"
     CDS             complement(4913..5806)
                     /locus_tag="U2K71_001425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM7315514.1"
                     /translation="MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYE
                     GNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGR
                     KIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQSIAELPLEQVKDTFM
                     INIIAMFALVKEAVPHIPAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQ
                     LAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLAS
                     NKASYVTAQIYGVTGGEAINL"
     gene            complement(5884..6806)
                     /locus_tag="U2K71_001426"
                     /pseudo
     CDS             complement(5884..6806)
                     /locus_tag="U2K71_001426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303839.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
     gene            complement(6855..7205)
                     /locus_tag="U2K71_001427"
     CDS             complement(6855..7205)
                     /locus_tag="U2K71_001427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEM7315515.1"
                     /translation="MEITMSKNAVETLIEKVGENTKIALALINDSDPFLRDKGAFAKG
                     SFFQIIPFVSEFGEYATKIEHPLLDIYTSKLEQNYFGKRLNMDFNKQLDSFSLENEIA
                     VLDYNIKLKNCFFS"
     gene            complement(7238..8140)
                     /locus_tag="U2K71_001428"
     CDS             complement(7238..8140)
                     /locus_tag="U2K71_001428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEM7315516.1"
                     /translation="MNKKEIAVVASCNTKFVPHLAALFVSVLDNCNPSKFVRFYVIDD
                     DIDFESKQLLRFSVKNARMNSDVEFLKINKEFFTNVVISDRIPETAYYRIAIPELFRG
                     TEVERILYMDCDMIALQDISKLWRLDFGDSIVAAVEDAGFHQRLEKMKISAKSMRYFN
                     SGLMLINVKKWLDENITQKVLDFIEHNPEKLRFHDQDALNAILHDRWLPLHPRWNAQG
                     YIMAKAKKHPTAAGEREYEETRNNPYIIHFSGHVKPWSKDFEGPTKKYYEKYAGMTAF
                     RCVAKFPKYPKYAKVQPKTDVVVK"
     gene            complement(8133..8990)
                     /locus_tag="U2K71_001429"
     CDS             complement(8133..8990)
                     /locus_tag="U2K71_001429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 8 protein"
                     /protein_id="HEM7315517.1"
                     /translation="MEKRYGVVPVVTASDENYAPYLSVMIATALENCNKTRRIKFYVI
                     DDGLSEYSKEGLEETVNKYSSNASIQFLTVEKDIYEDFLVSDHITTTAYLRISLPNLL
                     AKEDYKKVLYLDSDVLVLDDIVKLYDEPLNGKTIGAIIDPGQVKALERLGIDSDDLYF
                     NSGVMVIDIDQWNKKEITEKTIHYLSENGDRIIYHDQDALNAVLYEDWEQLHPKWNMQ
                     TSLIFERHPAPNEKYERQYKEGNEKPSIVHFTGHDKPWNTLKDHPYTNLYLKKLAHST
                     LTKVGEINE"
     gene            9324..10133
                     /locus_tag="U2K71_001430"
     CDS             9324..10133
                     /locus_tag="U2K71_001430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM7315518.1"
                     /translation="MKLAAIDLDGTLLDRNGKIPEENIQALRKFDENGGIVTVATGRN
                     SISAKDIFSELGIGGYLISSNGSLIAEMKDGKMAKVLRRSKIDSDTLRKAFFYAQEAK
                     ISIIASRETQDDQLTFDESNLVKDDPYYEHFNLQNHSFDQILNMLEDPSLSYLKLALT
                     DKSEEKLQKLQRDLQADGIQSVFSDPHFLEITPTGITKAHSLVFLADYLGIESSDIMA
                     FGDQENDIAMLNYSGLSIAMGNAQRQVKEIANQVTLTNEEAGVAYILKNFY"
     gene            10173..10670
                     /locus_tag="U2K71_001431"
     CDS             10173..10670
                     /locus_tag="U2K71_001431"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM7315519.1"
                     /translation="MLVIIDMQNHILDPNSEFYIPESDQLAERIAQRLAKARQNNEYI
                     LFTRDIPVELKDKEEEREDLQLIPLLSPQENERVIKKYYFTIPPETLAEIQETLFESK
                     EEQKEIEVVGIETNLCVLSNLIGLQSAFPEADFFVDPMLVSSRKHGESALELLKDFNV
                     SIVES"
     gene            complement(11055..11128)
                     /locus_tag="U2K71_001432"
     tRNA            complement(11055..11128)
                     /locus_tag="U2K71_001432"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(11092..11094),aa:Ile,seq:cat)
     gene            11315..11638
                     /locus_tag="U2K71_001433"
     CDS             11315..11638
                     /locus_tag="U2K71_001433"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315520.1"
                     /translation="MLGVIILPFIAILFDLVAAAAYFLQLNYQTSGILFIGMIFQGVI
                     TLLLLVMMLSYKGKRYARIQTKVFVKYVSIRYGIIILSFLVNAIVLFLYVLNYLDINP
                     LIFSR"
     gene            11802..12056
                     /locus_tag="U2K71_001434"
     CDS             11802..12056
                     /locus_tag="U2K71_001434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315521.1"
                     /translation="MITLVRVLFWVPAVALVASIVYLMNWNKERFYLAILTLPAIYFM
                     WKVFNYNYFEPDSVFIEELSGLVLSLLIVILYLIRLNKKH"
     gene            12126..12371
                     /locus_tag="U2K71_001435"
     CDS             12126..12371
                     /locus_tag="U2K71_001435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315522.1"
                     /translation="MSIEEAILLDTLRCRLNFLRTGRSLSQVAVSSALSIPLFKYRKY
                     EEGEEIPSIIELIYIADFYDISVDYLIGRSETASKLK"
     gene            12447..12812
                     /locus_tag="U2K71_001436"
     CDS             12447..12812
                     /locus_tag="U2K71_001436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315523.1"
                     /translation="MRLWHEKLISKLPRQQLLGQHRECCALRGNGWGKKHATVDYVFH
                     YSPYRLFQYHQLVMDEMEKRGYSPAPEWKNPMYRGKSCEAFFDLPTVERNVPIYPEHD
                     EVYLAECLANLEQKGIYLT"
     gene            complement(12873..13436)
                     /locus_tag="U2K71_001437"
     CDS             complement(12873..13436)
                     /locus_tag="U2K71_001437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315524.1"
                     /translation="MLYIKKGIIVISFFKEYLEIINFRLKTKKESVIILVYFQGGNLM
                     IKKLFLLSILTLAMAGCSDSGLATELITKSTEATTEELKCGNDHSDYRGLNRRCMGIL
                     GRSNCKHRCSNRRRCRRSNRKIKRKIKRSNLCCASYTDVEDAVLLNHVRRCSFMSREV
                     GDEPKLEYAFSFAREDEDNPKTYDILA"
     gene            complement(14004..14204)
                     /locus_tag="U2K71_001438"
     CDS             complement(14004..14204)
                     /locus_tag="U2K71_001438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM7315525.1"
                     /translation="MNNGTVKWFNADKGFGFITGEDGNDVFAHFSAIQGDGFKTLEEG
                     QAVSYDVEDGQRGPQATNIVKA"
     gene            14600..14749
                     /locus_tag="U2K71_001439"
     CDS             14600..14749
                     /locus_tag="U2K71_001439"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315526.1"
                     /translation="MTFYNKDIQYRVTLDTDLNLFVVFDKENTNHVATGVTIEQAVQE
                     LKKSA"
     gene            complement(14956..15669)
                     /locus_tag="U2K71_001440"
     CDS             complement(14956..15669)
                     /locus_tag="U2K71_001440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM7315527.1"
                     /translation="MNKIKLFIFDMDGLLLETGRLAYRAYVKSAQKYDYEMRKEVYYL
                     LTGQTEMAIREQMGILYGEDVPYIKWREAINQYKEKIVKEDKRVYTKKGAEEILSFAK
                     ERGIHTIVASSNTRVKVEMYLKMENLYALFDQIISGDDVKKGKPEPEIFLKACSKMNI
                     PPSEALVFEDSIAGIEAARRAGILSFLIKDHLIGLPDHNGKHKLKVDVSLYESNKADY
                     EFYDLHQAKRFLESKGLYL"
     gene            complement(15662..16747)
                     /gene="uxuA"
                     /locus_tag="U2K71_001441"
     CDS             complement(15662..16747)
                     /gene="uxuA"
                     /locus_tag="U2K71_001441"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HEM7315528.1"
                     /translation="MKWGFRWYGEKGDSIPLKYVRQIPGINGIIGTLLTKMPGDVWEI
                     SEIEELKASVESQGLELLGIESVAIHDAIKAGTDERDYYIDNYIRTIKNLSRCGVRLI
                     CYSFKPIFGWAKTDLFNENNDGSYSLLYDQKIVDQMEPGEMYQLIHSQSKGFKLPGWE
                     EERLKKFAQLEKLYEGMTEEELFENLTYFLEKVIPVCEKADVKMAIHPDDPPWELFGF
                     PRVTKNLGDLKKILNAVDSPYNGLTLCTGSLGANPENDLVEIIHEVGSRIHFVHFRNV
                     EFLGERKFKETAHPSTEGSLDMYHIMKALVQEGFDGPIRPDHGRTVWGEVAMPGYGLY
                     DRAMGLTYMQGLYEAISKEKPLGEHYE"
     gene            complement(16764..17207)
                     /locus_tag="U2K71_001442"
     CDS             complement(16764..17207)
                     /locus_tag="U2K71_001442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEM7315529.1"
                     /translation="MLEIMIVTHGKLSEGLKDAAEVIVGTSSHIETMCLESGEDIQTF
                     GEKVKEKLTMINTAEPMLVFVDLISASPYNQALLAVNELTEEKKAATYVIGGVNLPML
                     LEAINHQMLHTPIEEAVEQIVKQGKDNLTVWHASAEEKKLDEDDF"
     gene            complement(17241..17594)
                     /locus_tag="U2K71_001443"
     CDS             complement(17241..17594)
                     /locus_tag="U2K71_001443"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315530.1"
                     /translation="MLEMIRTIDDPSVAYAFVDEGCYGEKGLDSVRSGMKKEAILFYL
                     DSVGADTPLQFSGNYFSNKEQWLKQVDKLKEKNVNYIFSARKKQAQFFYLTKTDLRGK
                     TFNWQNANQIIALFR"
     gene            complement(17706..18107)
                     /locus_tag="U2K71_001444"
     CDS             complement(17706..18107)
                     /locus_tag="U2K71_001444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315531.1"
                     /translation="MKKNELFRDWEFRYRYIYRKRRTKKSKQRFLSALVSDIYSMRTD
                     VTVIAYDTLAYRSKNIYVGDIEKAEKVICTYYDTPVHALGSYFMFDWKDQRKKTIYSI
                     LLSFILLFSLGWWGMMIYNKNPHHVFDLLSV"
     gene            complement(18144..18653)
                     /locus_tag="U2K71_001445"
     CDS             complement(18144..18653)
                     /locus_tag="U2K71_001445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7315532.1"
                     /translation="MGVVNLARVDERLIHGQVMVTLSQKSGVNSIFVVDEVVAKDKFM
                     RDLYKNAGSRTGQKTIVITPEKAKFYWDEYQFKEYNCILIAKTVQTIYDLVKHGIPME
                     ELNIGGIAQKNPDTDLLVTKSVYLNKSDAEKLKEMNEQYGVKNIYFQATPSAPKTGLK
                     DVLNKFDLS"
     gene            complement(18675..19532)
                     /locus_tag="U2K71_001446"
     CDS             complement(18675..19532)
                     /locus_tag="U2K71_001446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM7315533.1"
                     /translation="MAEIMDNQTGAPEEITKKDVTKAYLRWHFANEIPHSFERYLAPS
                     LLYGMMPILKKLYKDEDSLKAAYKRQLLFFNTQLSWGGGVITGLMSSMEQQRAEEEYE
                     EKEILMQDDLMYNTKAGLMGALAGIGDAIDSGTVQYIFIAIAVPWAQQGSPLGALFPF
                     ICFGLYQVLLGVFFARQAFTMGRNATGLMQNTGVQTAIEMLSVLGLFMMGVLAGNYVK
                     VESSLKFAISGREFVIQEILDQIVPGILPLAVVMGVYLFYTKKGLKVTQALLWLTGIL
                     IVLAGIGIL"
     gene            complement(19550..20383)
                     /locus_tag="U2K71_001447"
     CDS             complement(19550..20383)
                     /locus_tag="U2K71_001447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7315534.1"
                     /translation="METLSVVQSLLIALWVAAVMSRWLGGGATLTLRFSPLMTGLVVG
                     LIVGEVAQAMIVTAALQMIYMGVFSPGGSMPAEPSIAAAIAVPVALLGDLSPEAAIAV
                     AVPVGLLGSYLYQFRFFINTFLGKYTDKAVEDLNTKAMARSIILYPTVASFLLFVPLV
                     FIALYLGAPLIADVIKALEGTVVIHILEVVGGGLAAIGIATTVYVIGRKDYLVFFFLA
                     YFMSVVLKSLDITMVTYAIFGIIIALIFVQSQKGKQVQPATSNNNSASFDDEDDYDDG
                     F"
     gene            complement(20397..21194)
                     /locus_tag="U2K71_001448"
     CDS             complement(20397..21194)
                     /locus_tag="U2K71_001448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM7315535.1"
                     /translation="MGYSANLARIDFGVIPTLASNCAPWTPLAVMYKENGQAEGKTEH
                     FYRQAAFFITDPQLVIDSPVPYFIAGLADTLAKWYESELILEQDFLQDESFLKLAQDT
                     AKICKEEILNHSAKAIQDMAQRKLTSEFKQLSEIIFAVAGLVGGLGDKYARNAAAHAM
                     HDAISKYIPESHRFLHGEKVAYGIFYQLALEGRWEKIDELYPLYRELELPVSLEQMGL
                     FPKDKQIIDKMAEFIDSKEKVHLIPIEVTQKSLIQSMMELEGYIRKI"
     gene            complement(21227..21511)
                     /locus_tag="U2K71_001449"
     CDS             complement(21227..21511)
                     /locus_tag="U2K71_001449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HEM7315536.1"
                     /translation="MMKKMVNGLKVKTGPQFYLYEEGGISKVSDLLKSYGAKRVLVTH
                     GTVSWEKALPKLVFLNDETIQFFYHRYSGECSYAEARRIATIIKKMKSIS"
     gene            complement(21508..22509)
                     /locus_tag="U2K71_001450"
     CDS             complement(21508..22509)
                     /locus_tag="U2K71_001450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HEM7315537.1"
                     /translation="MPDYHIAIVNSSSFGRVFPHHLERLKSVGDVHFFTVDQEIPGKE
                     LAELLKGFNIIIASVTPFFRRDFFENKDELLLISRHGIGYNNIDLKAAKEHQTIVSII
                     PALIERNAVAENNITNLLSMLRRTTQAQARVKDDRWEDRAEFVGRTLFNKTVGVIGAG
                     NTGSGVIEILRNGFRCDVLAYDPYKSDLYIESFGAKKVELQELLEQSDIICLCANLTE
                     ENYHMISDKEVDQMKENVYLSNSARGALVDEEAMINGLKSGKIAGYATDVLEEEPGRQ
                     DHPYLAFEQVVITPHISAYTRECLEAMGEKCVRDVEQISQHLLPERSVQTKSNYIKG"
     gene            complement(22511..23578)
                     /gene="gnd"
                     /locus_tag="U2K71_001451"
     CDS             complement(22511..23578)
                     /gene="gnd"
                     /locus_tag="U2K71_001451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HEM7315538.1"
                     /translation="MRIPHFHNKIIIFSVLINVKDTRRVGKNQTKNIFVKSIYKKIFL
                     YNEGIRNGGAIMEIGVIGLGKMGLNLALNLKEHHHKVQGLDISSTAVSQARENDIPAY
                     QEISEFLATFEQKKIIWLMLPAGEITENMLRKLFPKLGPGDIIIEGGNSYYKDSIRRA
                     EEFQKNEIGYLDCGTSGGIEGARHQACLMIGGDKETFLSVEQLFKDVAIERGYLYAGA
                     SGSGHFLKMIHNGIEYGMMQAIGEGFQLVQKSEYAFDLEQVARVWNHGSVIRGWLMEI
                     MEDQLNEHPNLSNYRGIVSASGEAKWTVETALEMAVPVPVIATSLFMRNLSQEEDSFS
                     AKVVSALRNGFGGHEIVEKGE"
     gene            23577..24425
                     /locus_tag="U2K71_001452"
     CDS             23577..24425
                     /locus_tag="U2K71_001452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM7315539.1"
                     /translation="MSVSIRLQIKTMYNDFSPKEQAIADYILENPSKVSHSPISNLAS
                     ELGIADSTFFQFTKKLGYNGFKDFKMAMLIQENDLSAISIHENIQKTDTELTMAQKVF
                     ESSISTLNDTKKLLQQKDLKKAATIINESKRVYFFGVGGSEIVATDAYHKFLRSPIAT
                     SHSTDYHIQLMEASLLTEKDCAVLISHTGQSKETIHIAETVRKTGAKTIVITSQANSS
                     LAKLGDVVFISISEETEFRSEALASRISQLSILDSLYVILMFYNEKESRRSIAKVRHT
                     ISEIKE"
     gene            25071..25277
                     /locus_tag="U2K71_001453"
     CDS             25071..25277
                     /locus_tag="U2K71_001453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3955 domain-containing protein"
                     /protein_id="HEM7315540.1"
                     /translation="MKAIHISLLFFASALILFAIHLCSQPYINNNGILVEPAFFCLPL
                     GFLSLLASAGFGFVAFFKRSKQQI"
     gene            complement(25479..26480)
                     /locus_tag="U2K71_001454"
     CDS             complement(25479..26480)
                     /locus_tag="U2K71_001454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM7315541.1"
                     /translation="MKWFKRIVIAVPVIIVVILVTVFLINQITPKPISLLVRKQFDTS
                     EKEQVYARPDDFQQKIEQIDIKKEISYPSKYKNNNMDIYTPKVEKKKLPILFWMHGGA
                     YVGGDKNDCRDYLEYLCSDTQQIIVNIDYERSPEAKHPTPVIQLNEAIQAAKKEIKDQ
                     ADWSNISIGGNSAGAQISGEYLLALQNEEIKKADHLDPTLENKQITKFISLSGLLDPS
                     ALTQVSDKISSFLYEKCGWAYFDNKDFEQSEKVKSLALVRHADRWTQNTFLTDGNTNT
                     FTKQMEEVTSVFEKAGVKVTKVDYAEKNAVLNHEYQFDFSNKQAQETYQKLVMFFKE"
     gene            complement(26485..28398)
                     /locus_tag="U2K71_001455"
     CDS             complement(26485..28398)
                     /locus_tag="U2K71_001455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEM7315542.1"
                     /translation="MNSLRTKQVLKGALVILFAVSTSVPQWTVYGESISSESESREVE
                     TTETTDAGNETKSSTSQTETNTETTVETSVTSTDQETTATTDNQQTEEATATKMSNDE
                     SSSAKVSDKENGTENDDIGKGENFNGKEYLNYFGKQMTEILNGPTEDIKSKLIELLVT
                     ELAVNDYGVDKEIIKHLIATYLLYDIAFAKNSVLMSMLGGTDDGTTPTENTGFYPNTK
                     QLHQTVLNKQDGKMMDVYAYYVDQGSDKTVMIHGGFRGNWNNGIVTEEYNDFYKAGYN
                     LLFVDSRATGNSGGDYVTYGQYESDDVLYWINQEVRERPSQKILLYGGSMGAATMMSV
                     LAKDIPVNVKGIIENCGFASIDEQLRFTYSQTVAPALPDAIKNQLDIIGDQEHEDLFM
                     GLLKQYYFDQEMHLVTKAALPTIGMSDSLPKLIIHGTADDVVPVSNAQKLYELSGGYK
                     DLLLVEGAGHGKAQEVDHAAYTKHVTDFLKVVFHDQINVKYVDENNKSLLKDQDEIQL
                     YGAYGENYMTEQKTFEGYELANVEGPTEGIFNETIPTIIFYYKKIPVAPPKKQDPTEN
                     ADNKGKNEPREDQSSDDTQPIQMTVKVQKKTDAKKRMLPKTGEKKHSLLIYSGGFLSV
                     STIITWIWRRKRG"
     gene            complement(28566..29072)
                     /locus_tag="U2K71_001456"
     CDS             complement(28566..29072)
                     /locus_tag="U2K71_001456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine hydrolase"
                     /protein_id="HEM7315543.1"
                     /translation="MSNTADAIVVIDLQKGVCFDSSPIHELETVTALVNKRIKTYETI
                     GKPVIFVQHEDAQLVPQTKPWELLSELSIPEHSYFVRKTHANSFYKTNLSVLLTELDC
                     HSIELCGAQAEYCIDTTVKFAHGLGYQLMMCRGATTTYDNPFMKAKETIAFYEKIWDK
                     RFLTFLEQ"
     gene            29232..29672
                     /locus_tag="U2K71_001457"
     CDS             29232..29672
                     /locus_tag="U2K71_001457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEM7315544.1"
                     /translation="MMIKLYTGSGCSSSRRAKRWLIVHDIPFIEQNLIRQPLTKNEFI
                     HLLKLTNNGVTEMLSTHSISYKNLVHRIDELSLSELYIEIRTSPSLLKKPIIYDQSAK
                     LLTGFSEDKIRMFLSKTKRKQELNELLLSGHLVDFKENNLFAMS"
     gene            complement(29698..30855)
                     /locus_tag="U2K71_001458"
     CDS             complement(29698..30855)
                     /locus_tag="U2K71_001458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315545.1"
                     /translation="MRTIQRKLRELNTILKNYHLSCSLKKGNPIEGEEYRIRYFFHLM
                     YWQSFDENHSDILSSIKNKRQLLEKFAKHAPYTRSIDRKKWISLLRISLQRMKQGHSI
                     TYVPEEMNKFVHPLITKEEFTHYILEPFFEANFWSMNHITPTEQAYLYFMFGVMNTYL
                     EEDLLDTPDKVQESWGWIESSIVENFEKNFKICFTTSEKNYLYVNLAMIHLYSRVFGT
                     KKKVDAFGKSAEDRDFQHIFPVMYPIMQQFFSGVARRIPELNTYYEKNRRLIFQYCML
                     VRDIFIKHEQPVIFYIQSKYGKTQELWAKKRILSMTERPIKYSASADQLPDIVISDYP
                     IDKKAYDQTNTQIFYWNGQPTKNDWARLKAEIIKIRNEKNRSILEKLDFVN"
     gene            complement(30858..31214)
                     /locus_tag="U2K71_001459"
     CDS             complement(30858..31214)
                     /locus_tag="U2K71_001459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315546.1"
                     /translation="MFEEFIDINERQVYQFLNYCYERDEKLYVVKDIALDLNYTLAKM
                     NSVIQQAESFCERYPEYKLSFLSENKMIKVEFSSQFLLSKVYSILLEGTIGYILLDSL
                     YKGTYQSLENLSQKII"
     gene            complement(31512..32486)
                     /gene="sgrA"
                     /locus_tag="U2K71_001460"
     CDS             complement(31512..32486)
                     /gene="sgrA"
                     /locus_tag="U2K71_001460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG-anchored fibrinogen/nidogen-binding
                     adhesin SgrA"
                     /protein_id="HEM7315547.1"
                     /translation="MKKTATVLFVILGLSIIAAMIGISTSANERANENLSFTVKTDRI
                     VYDMTQQVITIPVKPNKSVNASDVHAVLTYGWDGNGSSEKVIGEVYLKDVQWTAGIEY
                     TIMISAELSIDEIKSKDKVDLIVFYDGQMTITENLKPSSWTVVGPPSSTDSTSSESST
                     ENTSGESSTESTSSEISTENTSSESSTESTSSESSTGSTSSESSTESTSSESSTGSMS
                     SESSTGSTSSESSTGKVNNELDLVPIIPTKEQKRNAGSNLKSVLKQPDFVSRGIDHQE
                     KAKETDNRKSDLPETGSRTLNKLITWMGVLLILIVGASYFRSLFHRVK"
     gene            complement(32682..33521)
                     /locus_tag="U2K71_001461"
     CDS             complement(32682..33521)
                     /locus_tag="U2K71_001461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378019.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM7315548.1"
                     /translation="MIRIIRLIGSVLIVFVGFVLGILVNNALADMFIGNSEWIGAIAG
                     TVGHLAVFLLALFLASKVEGKRIEDYGVSVRGKDWGYLAGGLVVGIGVFLLITSPLYL
                     TGAYRLDSGNANIVPLITSFILFIAVGASEELLFRGFFQHQMLRFGPLIAMIGSAALF
                     ALLHGLNPNMTFLAVFNIFLAGCFFSALIYSTGSLFTAIGAHITWNWTQGAILGIPVS
                     GTQEQGFFSTLIQSQNTIITGGNFGAEASISTTLFLLVLIVGLLLYAYKTKRMKKYTA
                     VYQ"
     gene            33706..34593
                     /locus_tag="U2K71_001462"
     CDS             33706..34593
                     /locus_tag="U2K71_001462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HEM7315549.1"
                     /translation="MGNFLKKQLLIMCLSACAILLFSGCSKSNETIATFKGGTLTVQD
                     FYDNPKVKSNNQTLLKKMIVFKAFDDVYGKEISSKEVTKEYNEQIKKLGPNYKEQLKA
                     VGQTEETYKKLFKQMLAFQYGLKANVKLTDKDLDTAWKEFYPEVSTQIILFSTKEEAD
                     KAKKEANEGENFSKLVQAYGKNKTLKETDGKMNFDSTNPEIPTEVKKAAFKLKNGEVS
                     DIIPVTDPTTYQQSYYLVKMVKKQDKGSNKDKYKSELEKIATEAKTLDTEFMDKTIRK
                     VMKKDNVTIKDPYVKNIFK"
     gene            complement(35187..35881)
                     /locus_tag="U2K71_001463"
                     /pseudo
     CDS             complement(35187..35881)
                     /locus_tag="U2K71_001463"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628000.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="fructose-6-phosphate aldolase"
     gene            complement(35865..36263)
                     /locus_tag="U2K71_001464"
     CDS             complement(35865..36263)
                     /locus_tag="U2K71_001464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEM7315550.1"
                     /translation="MTIFETVVTDVGIEAEVFRKEKIMILFGDEAPETLTDYCYKIEL
                     NQVRKEITCDMKLCFDKQMYVITAVGDVVQTNLNELGHITIKFDGSHEPELPGTMYVE
                     DKEMPHLRKGTIIKIDQEDQNGGKLYGNHA"
BASE COUNT        10714 a         7219 c         5756 g        12717 t
ORIGIN      
        1 ttttcggaga attaaaaaat acaataaata actttagtta ctttaatgcg ttcttacttt
       61 atttttatat tatcattttt cttattcact tctttggttt atactattct tataaataaa
      121 aacgaggtat gtcctatgat gaaaagattg tactattcat tgatcataac gattggctat
      181 ttgattgtat caaatctagg aaatatggta tttggtatca gtaaagaatt cagttggact
      241 actacattat gggaatctct attctttttc atcttcgtat ttttgttaca aaattatcga
      301 aaaaaataaa catgtgccat ctatctgttg gcacatgttt taggatttat ggtacttttt
      361 attcggcaat ccattttatt tttctacttc ttcttaatac ctctaacact tttgatttat
      421 aaactaatcc aatgcatcta cttgcgctta ttaagctatt tcagctagat agtaccaccc
      481 catgcgtcaa tgcttgattt tccttttgta taacattgta taagcaataa aggacaaata
      541 agtggtgatt acaccaaacc attgaataag aaaaattctg atacgtaaat aaacatgaac
      601 ttgaaaaaat taatgaatcg gttagctact acgtagttgt cttttttctc atacagttct
      661 gttaacttac acttctcttc gctagataat aaaaaatcct ccaactgtat tgtccaatat
      721 aatcccaggc atctaaacta gcattataga agagacacac atagaatcta aaataactgg
      781 ttttaaatgt ttcttacaat caaaaaaagc tgttaaatgc actccacacc ttcatactct
      841 atcatttttt ataaacgata tatttttatt gtttttcgat aaaaaatagt tgttttacaa
      901 taattattct gctatatttt agctatcaaa gataaaagag ggataaaaat gaaatttaaa
      961 acattgctat taaaatttgt cgttttaggt ataagtgccg tatttatttt gttcggcgta
     1021 cttttttata ctcagatcgc tacaagcgaa aaaggattca attttgattg gcagaccaca
     1081 ttatttgttt ctgtcattta ctttgccctt ctcctaggga tcgttattgc ttatttctta
     1141 ttgaaactat tgaaaaaaat cgaagataac caagcttttt ccactcaaag cttaaacata
     1201 ctaaaaaaaa tcgaacgaac cattttgttt attggtttcg ccttttttgg cttacttcct
     1261 aaatttatga aagtagcgga tttagacgat gctccaggtt tgatgttggt aggaatcgct
     1321 gtcgtattca ttccttttgc agtatacaca ttaacaagcg tgctggaaaa actgcttgaa
     1381 catgcgattt tacttaagac agatcatgat ttgacagtgt gaggtgcgac atggcaatta
     1441 tcgttaattt agatgtagta ttagctcaaa aaaaaatgag tgtgactgaa ttgagtgaga
     1501 aagtgggcat tacgatggca aacatctcta ttctaaaaaa tggaaaagca aaagccatcc
     1561 gtttttccac tctcgaaaaa atctgccaag ttttggagtg tcaaccaggt gatttgttga
     1621 cctataaaaa tgactcgaat actataccag aagaataaaa taaagtagaa caaacagtaa
     1681 ataaaaaata atctgaatga tagatgtgtg agacttttag aagttattta cgtctattat
     1741 tcagattttt tatttcgatt gaataccgtt ttatctataa ataagatatg tactgactca
     1801 tacaaagggg gccttttctt ctccatgtac tatcactttt cctgatacaa ctggtttcct
     1861 gttacgttgt agttggatct gcttttagta taatacttta ttcattccac cagactgata
     1921 gcagtttgag cagtctaaat aatcatgtag tattacaggc cattctgttt cataccttga
     1981 agtgcttaaa acttcagcta atggcccatt gtgttaactc ttactccatt cttttttagt
     2041 aagctcccat atatactgta aagccttcta tatattatgt catgttgctt tttaacattt
     2101 actatgtagt tttgataaaa tgatttgaat aacagtgatg gacaattctt ttttattttt
     2161 aacattattt tgataataat gattatttat ttaaaaatac attagataac atttgttacg
     2221 acacgaaaaa gcttcacaaa caaatatcgt tattttaaaa tgagggcgaa ctcacacaat
     2281 taggaggaat agtatgccat ttattgttta cttttttctt tcattactcg tgatctatat
     2341 tccattacca acatttttcc tgctcgtcaa tcgtctagca gagcaacatg atacgattgc
     2401 tgctcttacg aaactttgtt tacttctatt tgtttgtcta gattacttta cgagctatta
     2461 ttggctaaaa cagacaagag taaaaaagag atattatttc ttccttgtct ttttcaatct
     2521 ctttgagttt ctgatccatt gctttttact tcatcagtat catacgatcc atttgtggat
     2581 catctgggtt ttcaaagtgg tcgtattatt ttgtatgttc acctttccta tctcaagaaa
     2641 atttagggag gcattgtttt attaaagaag cttctctgta tcaataaaga cacatttaag
     2701 gaaaaacttt taaaaatcta attgggaatt tgcccgcata aataagaggc tgtgacaaaa
     2761 gtcttgtcac agcctcttat tccgtataaa cagtgttcaa aagcttgcat cttcgataat
     2821 atgcccaacc aaatgaaaaa tatgagaagc tatttttgtt tggctgttct tattacttga
     2881 tgaagaacag ctttttcacg acctcttttt aaaagtcttt ttacagtttt cgagcaaaga
     2941 atgatacgat tagtactaaa atgatggcac caatcaatga tggcacgatt gccattcctg
     3001 caatacttgg tccccaagaa cctaataatg cttgaccaag tgctgatcca actaaacctg
     3061 ctacgacatt tgcaataatc cccatcgaag aacctttatt cgtgattgct cctgcaattg
     3121 ctccgataat tcctccgacg ataagtgacc aaataaatcc catgtttcgt tcctcctaat
     3181 gttttacttc cttattactg aacccgtggt tctgtatttt cttttacttt ttgtgcgccg
     3241 cttccagctg ctgattttac tttatctgtt tgattcgaag caaattcacc ggtttttttc
     3301 gctgcgtcag ttactttgtc ttgcaccgtt tcttgatctt tttcaaattc ttcttttgtt
     3361 ttgatgtcca ctacgttagc gttgacttcg atcacttcta aatgtgtcat attttttact
     3421 tctttgccga ttacttcttg catttcttca aagatttttt cgatatcttt gccatattcc
     3481 acaatgacat ccatatcaac tgctacttgt tttttaccga cttctgtttc gatcccagcc
     3541 gttacattat ctgtattcac aagttttcct gccacattag agaagaaacc accgtttgca
     3601 gataataaac cgtctacttg ttcaattgcg attccgatga ttttttgcac aactttatct
     3661 tcataattca gctcaccgcg cattgaatct tcctgttcag aatcatgctt tccttggtta
     3721 tttgtatgat cagaaccaag ttttccttgt ccgtttgtgt ggtcagaact aagtttttct
     3781 tgtgctttcg catggtcaga aggatgtttt tgctcttcca caggtttaga aggatcgatt
     3841 gcatcatgtt gttctggaac tttagattct ggagttcctg gatgatgagg tgcatgcttt
     3901 tctgcatcat gtttcttctc ttctactcct tgttgaacgt tagggactac tggatctgga
     3961 tgtgcagata ctggtgctgt tttgttcttt tcatcgttca caccatttga ttggtttggt
     4021 gtcgttgggt ctggatgtgc agacactggt ttttggttgt tcattttatt gttattattt
     4081 ttgttattgt tattcatgga tgtttcctcc taagatttaa aatagtgatc taagattttc
     4141 gtttgtttca gataaaaacc gatagcgacg ccgatcgctg ccatgatcaa gatcaataag
     4201 gtcttgaaaa agcccacggt gatccataaa acggctaata tcaatccaag taccccaaag
     4261 atgataggga gcttgtatgt cgaaaatagt tctttcattt ttggtccccc cctattctac
     4321 tcgtttgcgg tcattgcttc gtttagctgg ttcttcaaag cctgtgtatt tcaccgatac
     4381 tttcactggc tcttttgttc ccaatatgtg ttgtacttca ttttcgattt ctttcatcaa
     4441 tgttcctgtt ttaccaatga gtgctgaagt tcgtttcaac tcccctttta cattgacggc
     4501 gatcttgttt ttcgttgctt tgacatttac ttttggtgaa tggatgaatt catcttcatg
     4561 caagtgagaa cgaaccattc cttcaattgc ttttttatcc aacgtcaact ccccatcacc
     4621 atgtttcagt agaaagtttc cgattgtttt tggataaaac aggaaaacaa gtaacgtgat
     4681 aattgtcaaa atagcaaatg caacggcaag ccaaaataca tattgttgca cgtatgaatt
     4741 agtgatggaa aagaatcgaa acttctctag ctgaaaaggc aatggatagg ttgcacttaa
     4801 tagtatcgca aacaaaagca cagatagcaa aagtaatgtt ccgatcactc cgatcgtttt
     4861 tacaccttta ttcattttcg cacctcctca taggggtgaa acgatcaact tcttataagt
     4921 tgatcgcttc tccaccagtt actccgtaaa tttgggctgt aacataactt gctttattcg
     4981 atgctaagaa aacataaact ggtgctaatt ctcctggctg acctgcacgt tctagtaaac
     5041 tttgctgacc aaattcaggg atcgaatcag aaggttgtcc ttcgtctagt tgtaatggtg
     5101 tccagattgg accaggtgct acaccattta cacggattcc tttaggtgca agctgttttg
     5161 ctaaaccgat cgtaaagtta gcgattgaag cttttgttgc tgcgtaatct aataaatgtt
     5221 cgcttggatt gaatgcttga acagaagttg ttgttacgat tgctcctcct gctgggatat
     5281 gcggaacagc ttctttcact aaagcaaaca tagcgatgat gtttatcata aatgtgtctt
     5341 tgacttgctc aagtggtaac tcagcaatag actgacaaga aatttgctgt cctgcgttta
     5401 gtaccatcgt atctagcccg ccaagttcac tcacggcttt atctatgatt tctttaggtg
     5461 cttgttcgtc acgaagatca aaaggtagta agacaatctt acgtcctgca tctttcacgt
     5521 attttgctac ttcctctgca tctttttctt ctcctgggaa gaattgaaga gcgatatctg
     5581 ccccttctct agcaaatgca atcgctgctg cacgaccgat ccctgaatct ccaccagtga
     5641 ttagtacacg gcggttttct agttgattat tgccttcata ggattcttct ccacaatcag
     5701 gaacaggaat catattctgc tgtaaagctg gtgtatcctg atcttgttta ggaaatttct
     5761 ccgtatggta caagtttcgc gggtcgatta attttggttc tgtcattgct gtattcctcc
     5821 tagtttttaa attgtattgc caaaacaaat ttctttgttt gatacctgtt ttaaatagat
     5881 tctttaattt gtataatctt tccggtataa atggtaacct aaaactactg ctgctatcaa
     5941 agagccaaag actacgagta taaagcctag agcagaactt ttacccggta atcctcctgt
     6001 attcattccc caaatccctg cgaacaacgc cggaacggaa atgattaatg cagctgaatc
     6061 tagatatttc atgatatgat ttagattatt gtccatcatc ccgttgaata gatcaccaat
     6121 actctctaat aaatctcgat agatctcgat cattttttct gtttgtcgct gtctcaaatg
     6181 gacatcatag agtagctgtt catcgtctaa acgtttgatg aaagtttcag aattccataa
     6241 ttgatccatt gtttcccgtt gatctcttaa ggtatgatcg atatatacga tgtctctttc
     6301 aagatctgcc tgtttataca gttcttcatt ttccgttgtt ttacgagctg ctttatccaa
     6361 agcgtcaatt cgctttttca tctctcttag ttctttcacg taatgggtgt aaatatctaa
     6421 taatgtatac gtgatgatct tttcaaacga gtttaaatga tttccatgct ttgtcagcag
     6481 ccgatccaca aattcggttt ttctatcgat acaagtaatc aagagattat cggaaagtac
     6541 aaaagatatt ggcgttaatt ttttttcgat acgattatct gactgatcta gattaatcac
     6601 taccaaagaa aatggatgct ctagtttcgt ctcataaagg cgctccatcc gagaaacttc
     6661 ttccggatat tcggttccta cgaaaatatc atcaggaagc tcgaattctt cgattgcttg
     6721 ctcaatctct tcgggctttg cactatcaag tacgagccaa ttatattttt ttcatctgtt
     6781 tctattacat tcttttcttc atccacaaag tagtatctca atgtatacct cctttttgga
     6841 ctttctcgaa actattagct gaagaagcag ttctttaatt tgatattata atcgagtact
     6901 gcgatttcat tttctaatga aaagctgtca agctgtttgt tgaagtccat gttcaatcgt
     6961 ttaccgaaat aattttgctc taatttagat gtatagatgt ccaataatgg atgctcgatt
     7021 ttagtcgcat attctccaaa ttccgaaaca aaaggaatga tttgaaaaaa gctgcctttt
     7081 gcaaaagcac ctttgtccct tagaaatgga tcggaatcat tgataagcgc gagtgcgatt
     7141 tttgtatttt ccccaacctt ttcaattaac gtctctactg catttttaga cattgtaatt
     7201 tccattccta ctccccctct tcagttgtgg tcttctatta tttaacaact acatctgttt
     7261 ttggttgaac ttttgcatat tttggatatt tagggaattt agctacacaa cggaaagccg
     7321 tcatccctgc atatttttcg taatatttct tcgttggccc ttcaaaatcc ttgctccacg
     7381 gtttcacatg tcctgaaaaa tggataatgt aaggattgtt gcgagtttct tcgtattcac
     7441 gttcacctgc tgctgttgga tgtttttttg cttttgccat gatatagcct tgtgcattcc
     7501 aacgcggatg aagtggtaac caacggtcat gtaagatagc atttaatgca tcttggtcat
     7561 ggaatcgtaa tttttctgga ttatgttcga tgaaatctaa tactttttgc gtaatatttt
     7621 catctaacca ttttttcaca ttgatcagca tcaaacctga gttgaaataa cgcattgatt
     7681 tagctgaaat tttcattttt tccagtcgtt gatggaaccc agcgtcttca acagctgcta
     7741 cgattgaatc accgaagtca agacgccata atttagagat atcttgtaaa gcgatcatgt
     7801 cgcaatccat atataagata cgttcaactt ctgtgccgcg gaacaattcc ggaatcgcaa
     7861 tacgataata agctgtttct ggaatacgat cgctgattac cacatttgta aagaattctt
     7921 tattgatttt caagaattcc acatctgagt tcattcgtgc gtttttcact gagaagcgta
     7981 aaagctgttt actttcaaaa tcgatatcat cgtcaatcac atagaaccga acaaatttag
     8041 acgggttgca attatctaat actgagacaa ataatgcagc taaatgtggt acgaattttg
     8101 tgttacaact tgcgactact gctatttctt ttttattcat tgatctcgcc tactttcgtt
     8161 agagtactgt gtgctaattt ttttaagtat aaattggtat acggatgatc tttcaacgta
     8221 ttccaaggtt tatcatgtcc tgtaaagtga acgatcgaag gtttttcatt tccttcttta
     8281 tactgacgtt catatttttc atttggtgct ggatgacgtt caaaaatcag cgaagtctgc
     8341 atgttccatt ttggatgaag ttgttcccaa tcttcataaa gtacagcatt caaagcatct
     8401 tggtcgtgat agatgattcg atcgccattt tcactgaggt aatggatagt tttttctgta
     8461 atttcttttt tattccattg atcaatgtca atcaccatca ctcctgaatt gaagtacaag
     8521 tcatcggaat cgattcctaa tctttccaaa gctttcactt ggcctggatc gataatagcg
     8581 ccgattgttt ttccatttaa cggctcatca tatagtttta cgatatcgtc tagtactaac
     8641 acatctgaat ctaagtaaag gactttctta taatcctctt tagctaataa gtttggtaaa
     8701 gaaattctta agtaagcggt agttgtgata tggtcactga ctaggaagtc ttcgtagata
     8761 tctttttcca ctgtcaggaa ttggatactt gcattacttg aatatttatt taccgtttct
     8821 tccaatcctt ctttactata ttctgataga ccatcgtcaa tcacataaaa ttttatgcgt
     8881 cttgttttat tgcaattttc taatgcagtt gcgatcatca cgctcaagta aggagcataa
     8941 ttttcgtctg aagcagttac cacaggaact acgccgtatc tcttctccat caaaatcact
     9001 cctttttcta atctttttct aacctgttct acatctagaa tattcctcat ttggttttaa
     9061 agcaaaatat atgctttgtt ttcattgaat attttaaaat ttttttatca caaaacgcat
     9121 cggtaagcgc taactattcg tttttcatgg ctttttattc aaaggtattg accaaaaact
     9181 actatatata gccacctttt tcttaagaat gttttttgaa aaaattcata atgacaaagc
     9241 aagttagttt cattttttta aattctcatt taatcttaag gtaacaggaa aatagatatg
     9301 taaaaagaaa gtaggaagat aaaatgaaac tagcagcgat tgatttagac ggtaccttgc
     9361 ttgatcgaaa tggaaagata ccagaagaaa atatccaagc acttcggaaa tttgatgaaa
     9421 atggcggaat cgtaaccgtc gctaccggaa ggaacagcat ttctgctaaa gatatttttt
     9481 ccgaattagg aatcggtggc tatttgattt cttccaatgg ttcactgatt gctgaaatga
     9541 aggatggaaa aatggcgaaa gttctcagac gttcaaaaat agatagcgat acactgagaa
     9601 aagccttctt ctatgctcaa gaagcgaaaa tatccatcat tgctagccga gaaacccaag
     9661 atgatcagct gacattcgat gaatcaaatc tagtcaaaga tgacccttat tatgaacatt
     9721 tcaatttaca gaaccattct tttgatcaaa ttttaaatat gttggaagat ccttctctta
     9781 gctatcttaa attagcatta acagataaga gtgaagaaaa actacagaag ctacaaagag
     9841 atctgcaagc agatggtatc caatcagtat tctctgatcc gcatttctta gaaatcacgc
     9901 ctacaggtat cactaaagct cattcacttg tttttctggc ggattacctt ggaatcgaat
     9961 cgagtgacat catggctttt ggcgatcaag aaaacgacat tgctatgttg aattacagcg
    10021 ggttgagtat tgcgatgggg aatgcacagc gacaagtaaa agaaattgca aaccaagtaa
    10081 cactgacgaa tgaagaagct ggtgttgctt atattttaaa aaacttctat taaaaagaat
    10141 aaaatgacga tatgaatgta ggaggaaaga aaatgttagt tattattgat atgcaaaatc
    10201 atattcttga tccaaacagc gaattttata ttccagagtc agatcaattg gcagaacgta
    10261 ttgctcaaag attagctaaa gcacgccaaa ataatgaata tattctcttc acacgggata
    10321 taccagttga actgaaggac aaagaagaag aacgagaaga tttgcaattg atccctcttc
    10381 tttctccaca agaaaatgaa cgagtgatta aaaagtatta ctttacgatt ccacctgaaa
    10441 ctttagctga aatccaggaa actttatttg aaagcaaaga agaacaaaaa gagatcgaag
    10501 tagtcgggat tgaaacaaac ttgtgtgtac tctctaatct gataggcctg cagagtgctt
    10561 ttcctgaagc agactttttt gttgatccaa tgttagtctc cagtagaaaa cacggagaat
    10621 ccgctttaga gctgttgaaa gatttcaacg tctctatcgt cgaatcataa aaatatctat
    10681 cttcatttac gatatttaac acacaataaa aaaagcacgc agaactgttt tagtcctacg
    10741 tgctttttct atcaaagtag attggctttt ttatgacaca gagtgaaacg gcaggctcac
    10801 gacctctttc ttaaatggtg ttcaatcagc tgaccctcgg tattagctta acaaacggca
    10861 aaatatgagg aactattttt ccgtttgtta agctaatact caggtcaaaa cgctgatttc
    10921 acaacctctt ccttaaacgg tattcaaaag cttgcatctg cagtaataag aacagccaag
    10981 taaaaacata agaagctgtt tttacttggc tgttcttatt acttgatgca gggcagcttt
    11041 ttcacaacct cttatgtacc ttgtaggact cgaacctacg accggacggt tatgagccgt
    11101 ctgctctaac caactgagct aaaggtacat cataaataaa atttattttt gttacttttc
    11161 aactatagca cgaatgacaa actctcacaa gtttcaaaat ataagtaaaa taatattgtt
    11221 attcttattt cttttacttg tgtcataact attttgttac tttaattttt atttgcttat
    11281 tttagaaaaa atgttattct tttatttatt ctcaatgtta ggagtgatta ttttgccatt
    11341 tattgcgatt ttattcgatt tagttgcggc agcagcttat tttctccaat taaattatca
    11401 aacctcagga attctgttta ttggaatgat ttttcaaggt gtgattaccc ttttgcttct
    11461 tgtcatgatg ctcagctata aaggaaaaag atatgcccgt attcaaacaa aagtatttgt
    11521 taaatatgtt tccattcgct atgggatcat cattctttcc tttttagtga atgcgatcgt
    11581 cctcttttta tatgtattga attacttaga tatcaatcca ttgatttttt ctcgatagat
    11641 aagacacatt cagcggaaca gttcctgctt atcagcagaa aactgttctg ctttttcatt
    11701 tttcattgat ctattcttct tttattttcc tagcatttca tttgtgatgt atgcaataac
    11761 atgttatctt ttattatatg aatcgttgag gagtccgtta catgattacg ttggtgagag
    11821 ttttgttttg ggtgcctgcg gtcgcactgg tcgcaagtat tgtttatctt atgaattgga
    11881 acaaagaacg gttttattta gctattttga ctcttccggc tatctacttt atgtggaaag
    11941 tttttaatta caactacttt gaacccgata gtgtttttat agaagaatta tcaggactgg
    12001 ttttgtcttt attgatcgtc attctttatt tgatacgttt aaataaaaaa cattgagtac
    12061 tattgattgc atgtgtagct ttctatagtc attataacta ttctactata atgaaaggtg
    12121 attaaatgag tatcgaagaa gctattttac tggatacttt acgttgtcgt ctgaatttct
    12181 taagaactgg ccgatcttta tctcaagtag ctgtttctag cgcgttaagt attcctttat
    12241 tcaaatatcg taagtacgaa gaaggagaag aaattccaag catcattgaa ttaatctata
    12301 tcgctgactt ttacgatatt tccgttgatt acctaattgg tcgctcagaa acagcaagca
    12361 aactgaagtg attttttcca gccttggtgc tggttttttc atttaacgtc taaggttaac
    12421 aatctatata taaaggagac atttctatgc gattatggca tgaaaaattg atttcaaagc
    12481 ttcctcgtca acagctttta ggacaacatc gggaatgctg tgcattaaga ggaaatggct
    12541 ggggaaagaa acatgctact gtggattatg tttttcacta ttctccttat agattattcc
    12601 aatatcatca gcttgtcatg gatgaaatgg aaaaacgtgg ttacagccca gcaccagagt
    12661 ggaaaaatcc tatgtatcgt ggcaaatcat gcgaagcatt ttttgattta cctactgtag
    12721 agcgcaacgt gccgatttat ccggaacacg atgaagttta tctagcagaa tgtttagcaa
    12781 atttggagca aaaaggaatt tatcttacct aatataacta gaaaagcctt ctatctcctc
    12841 ttacttcatt aaaaagcttt aaccatctta gatcaagcta aaatgtcgta agttttaggg
    12901 ttatcttcat cctcacgtgc aaaactaaaa gcgtactcta gttttggttc atcgccaact
    12961 tccctagaca taaatgaaca tctcctgacg tggtttaaaa gtaccgcatc ttcgacgtct
    13021 gtatacgatg cacagcacag attgcttctt ttgatctttc ttttgatttt tctgttgctt
    13081 cttctacacc ttcttcgatt actacatcga tgcttacaat tgcttctgcc taatattccc
    13141 atgcatcgtc gatttaagcc tctgtagtca ctgtgatcgt ttccacattt taactcctct
    13201 gtcgtggctt cagttgattt tgtgatcagt tcagttgcta agccagagtc agaacaacct
    13261 gccattgcta gcgttaatat tgatagtaaa aatagttttt ttatcataag gttccccccc
    13321 tgaaaataga ctaaaataat aacgctttct ttttttgttt tcagacgaaa attaataatt
    13381 tctaaatatt ctttaaaaaa gcttataact attatgcctt ttttaatata taacacagtt
    13441 atttttaaaa ataagaatgc taaatcactc tacaataacg gcgaaagcac aaaaaataaa
    13501 aaaattatta cgttattaaa aaaacagatg aaagatcagg ttttattaga tctcattctt
    13561 tcatcacttg aagggattta atgaactact ttttcctctt tgtcgttttt atatattcgt
    13621 attccttcaa acagtaggat agcctctttc aatgcttgat atttgaactg tccgttactt
    13681 aaaaatttag atgtgccgcc ctcttcctag aacatattct tttttacttt ttcattaatt
    13741 gacagaccaa tcgaacgaaa ctgtaaattt cgataggata atgattggtc aaactcattc
    13801 attttcaaga catctcgatt catattcaag ataggaatct ttttatcaac atcactttat
    13861 tcgtattttc ctctaccata aaagcttcac gctccacaaa attgttttat acactactat
    13921 tcagcccatt caacgaccaa tgaatttttt cgtacaaaaa aaccgtcttt tctttcgaaa
    13981 agacggttaa aagtcaattt ttattaagct ttaacaatgt ttgttgcttg agggccacgt
    14041 tgaccatctt caacatcgta agacactgct tggccttctt ctaaagtttt gaagccgtca
    14101 ccttggatag ctgaaaaatg tgcaaataca tcatttccat cttcaccagt gataaagcca
    14161 aaacctttgt ctgcgttgaa ccattttact gtaccgttat tcataaataa attcctcctg
    14221 atacgtatca atgtacgttt tttcgttgca ataaatattt gataggcaaa aggagtttta
    14281 tctatgtaga tatatcgctc aaattacgtt tcaaaataga ttactcattt aatatatcat
    14341 gctttttcta ataacacaag gataatgcaa tgaaatataa aattcattgt cttctattgt
    14401 cttcgaatcg aagaaaaagt tgaataaccg cgattgattc actttttgtt ttcttgttta
    14461 tttgctttta tctgcaactt ttgactttaa aatttcttaa ctatgaaaac ctttcaattg
    14521 gtggtatact tataaatgga atttcgttat caacgcgttc gttgcctttc agcaagtatt
    14581 taaagaaagg agaatggcta tgacattcta taacaaagat atacaatatc gagtaacctt
    14641 ggatacagat ttgaatctat ttgtcgtttt tgacaaagaa aataccaatc atgtagcgac
    14701 aggtgttacg atcgaacaag cagtgcaaga attaaaaaaa tctgcataag gagtggaaac
    14761 cctcatatgg acgattctcg ttcttctctt taaacgcatc tactggtaga taacagtaga
    14821 tgcgttttgt gtttgttcta aattgttcac tacaaataag gtagatactt caaaataaaa
    14881 aattacttct ttttcatctg ttccctcagt cgtgcaggac aataaataaa gaggactaga
    14941 aaatagtcct ctttattaga gatacaaccc tttactttct aaaaatcgtt ttgcttgatg
    15001 caggtcataa aattcatagt ctgctttatt ggattcatat aaacttacat ctactttcaa
    15061 cttatgcttc ccattatgat cgggcaaccc tatcaaatga tctttgatga gaaaagacaa
    15121 aatgcccgct cgccttgctg cttcgatccc tgcaatcgag tcttcaaata caagtgcttc
    15181 acttggagga atattcattt ttgaacaagc tttcaaaaag atctctggtt ccggctttcc
    15241 tttttttaca tcatcccctg aaatgatttg atcaaacaag gcatatagat tttccatttt
    15301 taaatacatt tctactttta cccgtgtatt agacgaagcc acgattgtat ggattcctct
    15361 ttcttttgca aaagataaaa tttcttctgc tccttttttc gtatatactc tcttatcttc
    15421 tttcacgatt ttttctttat attgattgat tgcttctcgc catttgatat atggaacatc
    15481 ttctccgtac aaaatgccca tttgttctct tatcgccatt tctgtttggc cagttagtaa
    15541 ataatagact tccttacgca tttcataatc atacttttgt gctgatttta cataagcacg
    15601 ataagctaaa cgtccagttt ctaataaaag gccatccata tcaaagatga agagcttgat
    15661 tttattcata atgctctccc agtggttttt ccttacttat cgcctcatat agaccttgca
    15721 tgtaagtgag tcccattgca cgatcataca ggccatatcc aggcatagcc acttcacccc
    15781 acacggttct accatgatca gggcgtatag gaccgtcgaa cccttcttgc accaacgctt
    15841 tcataatatg atacatatca agggagcctt ctgtagaggg atgtgccgtt tctttgaact
    15901 ttctttctcc taaaaactcc acgtttctga agtggacaaa atggatgcgc gaacctacct
    15961 catggatgat ttcaactaaa tcgttttccg gatttgcccc taaagatcct gtgcacagcg
    16021 ttagtccatt atatggagaa tctactgcat ttagaatttt cttcaagtcg ccaagatttt
    16081 tcgtcactct aggaaaacca aaaagttccc atggcggatc atctggatgg atagccattt
    16141 ttacatctgc tttttcgcac actggaatca ctttttctaa aaaataagtc agattttcaa
    16201 ataattcttc ttcggtcatt ccttcataca gtttttccag ctgagcgaat tttttcaacc
    16261 gttcttcttc ccaacccggc aatttgaatc cctttgactg gctgtgtatc aattgataca
    16321 tttctcctgg ctccatctga tcgacgattt tctgatcgta taaaagagaa tagcttccat
    16381 cgttgttttc attaaacaaa tcagttttcg cccatccaaa aattggtttg aagctataac
    16441 aaatcaatcg aaccccacat cgagacaagt ttttgattgt tcgtatatag ttatcaatat
    16501 agtaatctcg ttcatctgtc cctgccttga ttgcatcgtg gatcgctaca ctttcaattc
    16561 caagcagttc cagtccttgg ctctcaacag atgctttcaa ctcctcaatt tctgaaatct
    16621 cccaaacatc tccaggcatt ttagtcagca aagttccaat tatcccattt attcccggta
    16681 tttggcgaac atattttaat gggatcgaat cccctttttc accataccat ctaaatcccc
    16741 acttcatctt ttcagccttc cttctaaaaa tcgtcctcat ctaatttttt ttcttccgct
    16801 gaagcatgcc aaacagttaa attatctttt ccttgtttga caatttgttc caccgcttct
    16861 tctatcggtg tgtgaagcat ctgatgattg attgcttcta acaacattgg cagattcacg
    16921 ccccctatga cataagtggc tgctttcttt tcttcagtca gttcattgac tgccaagagc
    16981 gcttgattgt acggacttgc gctaattaga tcaacaaata ctagcatggg ttctgctgtg
    17041 ttgatcatag ttaatttttc tttgaccttc tctccgaatg tttggatgtc ctccccagac
    17101 tctaagcaca ttgtttcaat atggctcgat gtcccgacaa ttacttccgc cgcatctttc
    17161 agtccttcgc tcaacttacc atgtgtaacg atcatgattt ccaacattag tcttctcact
    17221 ccttgttttg cactttttac ttatcgaaaa agagcaataa tctgattggc attttgccaa
    17281 ttaaatgttt tccctcgtaa atctgttttg gtcaaataga agaactgcgc ttgctttttt
    17341 cttgcactaa aaatataatt gacgtttttt tctttcaact tatcaacttg tttcagccac
    17401 tgttctttat ttgaaaaata atttcccgaa aactgtagcg gtgtatctgc cccaacagag
    17461 tctagataaa aaagaatagc ctcttttttc atacccgatc tcacgctgtc taagcccttt
    17521 tctccataac agccttcatc aacaaacgca tacgctacac tcggatcatc aatcgtacgg
    17581 atcatttcta gcatcgttaa tatggatgaa gtatttcgaa taaaagtttg tctgctaggc
    17641 catccgcgag ctgcttttcc caataagaaa aagtagctcc caaatgctag tacagtgata
    17701 cctgtttata cggatagcag atcgaatacg tgatgaggat ttttgttata tatcatcatt
    17761 ccccaccaac ctaaactgaa caacaaaatg aaggacaata atatagagta gattgttttt
    17821 ttcctttggt ctttccaatc aaacatgaaa taacttccta atgcatgaac gggtgtatcg
    17881 taataagtac agataacttt ctctgctttt tcgatatctc caacataaat attttttgat
    17941 cggtaggcta gcgtatcgta agcaatgact gtcacatctg ttctcatact gtaaatatct
    18001 gatactaatg ctgaaagaaa acgctgttta ctcttttttg tacggcgctt acggtaaata
    18061 tagcgataac gaaattccca atcacgaaac agctcgttct tcttcatttt ttcgccctct
    18121 ttatcttttt tctaagcatt tgtttatgac aaatcaaact tgttcaatac atcttttaat
    18181 cctgttttag gtgcagatgg agtggcttga aaataaatat tctttacgcc atattgttca
    18241 ttcatttctt tcaatttttc agcatctgat ttatttaaat aaacagattt cgtcactaaa
    18301 agatctgtat caggattttt ttgagcaatc cctccaatat tcaattcttc catggggatt
    18361 ccgtgtttta ctaagtcgta aatcgtttgg actgtttttg cgatcaaaat acaattgtat
    18421 tctttaaatt gatattcatc ccaatagaat ttagctttct ctggtgtaat gacaattgtt
    18481 ttttgtcctg tacggcttcc tgcatttttg taaagatctc tcataaattt atcttttgct
    18541 acaacttcgt ctacaacaaa aatggaattc actccacttt tttgagataa cgttaccatt
    18601 acttgcccat ggatcaaacg ctcatccaca cgcgctaaat tgactacacc catcttattc
    18661 acgttccctt ctttttataa aataccgatt cctgctagca caattaatat tccagtcagc
    18721 cataataatg cttgtgtgac tttcaatcct tttttcgtat agaacaagta cacacccatt
    18781 acgacagcta aaggtaatat ccctggcacg atttgatcaa gaatttcctg gataacaaac
    18841 tcacgtccgg atatcgcaaa tttcaatgaa gattctactt ttacatagtt tcccgcaagc
    18901 acccccatca taaacaaccc taaaactgat agcatttcaa tcgctgtctg aacaccagtg
    18961 ttttgcatta ggcctgtagc attacggccc atagtgaaag cttgtcttgc gaaaaatacc
    19021 cctagtaaaa cctgatacaa cccaaagcag ataaaaggaa aaagtgcgcc taaaggactt
    19081 ccctgttgtg cccaaggtac cgcgattgcg ataaaaatat attggacggt tcctgaatcg
    19141 attgcatctc cgattcctgc tagtgccccc atcaatcccg ctttcgtgtt atacatcaag
    19201 tcatcctgca tcaagatctc tttttcttca tactcttctt ctgctctttg ctgttccata
    19261 gaagacatta ggccagtaat cacaccgcca ccccagctta attgagtgtt gaagaacagt
    19321 aactgccgtt tatatgctgc ttttaaagag tcttcatctt tataaagttt tttcaagatt
    19381 ggcatcattc cgtatagtag tgatggcgcc aagtaacgtt caaaagagtg aggaatttcg
    19441 tttgcaaaat gccatctcag ataggctttt gttacatctt ttttcgtgat ttcttcagga
    19501 gcacctgttt gattatccat tatttcagcc atttcccatt cctcctaaat taaaatccgt
    19561 catcatagtc atcttcgtca tcaaaagatg ctgaattgtt attgcttgtg gcaggctgta
    19621 cttgtttccc tttttgcgac tggacaaaga tcaaagcgat aataatccca aaaatggcat
    19681 aagtaaccat cgtaatatct aaagatttca aaacaacact cataaaataa gcaaggaaga
    19741 aaaagaccag ataatctttt cgaccgataa cgtatacagt tgtcgcgata ccgattgccg
    19801 ctaaccctcc acctactact tcaagaatgt ggataacgac tgttccctct aaagctttga
    19861 ttacatcagc gatcaatggc gcccctaaat ataaagcaat gaaaacaagc ggaacgaata
    19921 ataaaaatga ggcaaccgtt ggataaagaa taattgatcg ggccatagct tttgtattca
    19981 aatcttctac agctttgtct gtatatttac ctaaaaaagt attgatgaaa aaacgaaact
    20041 gatacaagta acttccgagc aatccgactg gcaccgcaac ggcaatcgct gcttctggcg
    20101 acagatcccc taataatgcg actggtactg cgatagctgc tgcaatagat ggttcagctg
    20161 gcattgatcc tcctggagaa aaaaccccca tataaatcat ttgcaaagcg gctgtaacaa
    20221 tcatcgcttg tgcaacttcc cccacgatca acccgacgac taatccagtc atcaaaggag
    20281 aaaaacgtaa ggtcaacgtg gctcctccac ctaaccagcg ggacataacc gcggctaccc
    20341 ataaagcaat caacaaactt tgtactacac ttaatgtttc catgtgtcct cctccttcag
    20401 atctttctga tataaccttc cagttccatc attgactgta tcagtgattt ctgtgttacc
    20461 tctataggaa tcaaatgcac tttctctttc gaatcaataa attcagccat tttatctatt
    20521 atctgtttat ctttcggaaa taatcccatt tgttctagag aaaccggcag ttctaattcc
    20581 cgatacaaag gatataactc gtctattttt tcccagcgac cttctagtgc cagttgatag
    20641 aaaatcccat acgctacttt ttctccatgt agaaaacgat ggctttccgg aatatattta
    20701 ctgatagcgt catgcatagc atgtgctgcc gcatttcttg cgtatttatc tcctaatcct
    20761 ccaactaaac cagcaacagc aaagataatc tctgaaagct gtttgaactc cgatgtcagt
    20821 tttctttgtg ccatatcttg gattgcttta gcagaatgat tcaaaatctc ttctttgcag
    20881 atcttcgcag tatcttgtgc tagcttcaaa aatgattcat cttgcaaaaa atcttgttct
    20941 aagatgagct cagattcata ccattttgct agtgtatctg ctaacccggc aatgaaatat
    21001 ggaacagggg aatcaatcac taattgggga tctgttataa aaaaagcagc ttgtctgtaa
    21061 aagtgttctg ttttcccttc tgcttgtcca ttctccttat acatgacagc aagaggtgtc
    21121 catggagcac aattactagc taaagtaggg atgactccga aatcgattct tgcaagattg
    21181 gcactataac ccactagatc ggtcagtttt cctccgccaa cgccaactac gaaatcgatt
    21241 tcattttttt gatgattgta gctattcgtc tggcttcagc ataactacat tctccagaat
    21301 aacgatgata aaaaaattga atcgtttcgt cattcaaaaa tactaattta ggtagcgcct
    21361 tttcccatga taccgttccg tgggtgacca gtactctttt cgcgccgtat gatttcaaga
    21421 gatcagaaac ttttgaaata ccaccttctt catataaata aaattgaggg cctgttttca
    21481 cttttaaacc gttcaccatt ttcttcatca tccttttata tagttgctct ttgtctggac
    21541 agaacgttca ggaagaaggt gctgactgat ctgttcgaca tctctgacac atttttcacc
    21601 cattgcttct aaacattccc ttgtgtaggc cgatatatgt ggcgtaatca ctacttgttc
    21661 aaacgctaaa tacggatgat cttgacgtcc cggttcttct tctaaaacat ctgtggcata
    21721 tcctgcgatc tttccgcttt tcagaccatt aatcatcgct tcttcatcaa ctagagcgcc
    21781 tcttgcacta ttagatagat aaacattttc tttcatctga tcgacttctt tatcagaaat
    21841 catgtgatag ttctcttctg tcaaattcgc acataaacaa atgatatctg attgttccag
    21901 taattcttga agttctactt tttttgcacc aaagctttcg atgtataaat ctgatttgta
    21961 aggatcataa gctagaacat cacagcgaaa accgttacgc aaaatttcga ttactccgct
    22021 tccggtattt cctgcaccaa tcacaccaac tgttttgtta aacagcgtcc taccgacgaa
    22081 ctccgcccga tcttcccatc gatcgtcctt gactcttgct tgtgcttgtg tcgtgcgtcg
    22141 aagcatggac aacaaattag ttatattatt ttctgctaca gcatttcgtt caattaaagc
    22201 aggtatgata gatacgattg tctggtgttc ttttgctgct tttaaatcaa tattgttgta
    22261 accaatacca tgtcgggaaa tcaatagcag ttcatctttg ttttcaaaaa aatctcgtcg
    22321 aaaaaatggc gtaacacttg caataatgat attgaatcct ttaagtaact ccgctagttc
    22381 ttttcctgga atctcttgat caacagtaaa gaaatgaacg tctccaactg actttaatct
    22441 ttccaagtgg tgtggaaaga ctcttccgaa actgctggaa ttaacgattg ctatatgata
    22501 atctggcatt ttactcaccc ttttcaacta tttcatgtcc gccaaaccca tttcttagtg
    22561 ctgaaactac ttttgctgaa aaactatctt cttcctgtga aagattcctc atgaacaaac
    22621 tagttgcaat tactggaact ggaacagcca tttctagcgc tgtttcaacc gtccattttg
    22681 cttcacctga tgcagaaacg attccccgat aattacttaa attgggatgt tcgtttaact
    22741 gatcttccat gatttccatc agccagccgc gaatcaccga accatgattc caaacacgtg
    22801 cgacttgttc caagtcaaaa gcatactcac ttttttgtac aagttggaat ccttcaccga
    22861 ttgcttgcat cattccatat tcgattccat tatggatcat tttcaagaag tgtccgcttc
    22921 cggatgcacc agcatacaga tatcctcttt caattgctac atctttgaat aattgttcca
    22981 cggatagaaa agtttcttta tctcctccaa tcattaagca ggcttgatgt cgtgctcctt
    23041 cgattcctcc agaagtccca caatccaagt aaccgatttc atttttctga aactcttctg
    23101 ctcgacggat tgaatcttta taataagagt ttccgccttc aataataatg tctcctggtc
    23161 cgagttttgg aaaaagtttt cttaacatgt tttcggttat ctctccagca ggaagcatta
    23221 accagatgat ttttttctgc tcaaaagtag ctagaaattc agagatctct tgataagcag
    23281 gaatgtcatt ttctcttgcc tgactaacag ctgtacttga aatgtcgagt ccttgtactt
    23341 tgtgatggtg ctcttttaag tttaaagcta aattcaatcc catttttccc aaaccaatta
    23401 caccaatttc catgatagcg cctccatttc ttataccctc attatacaaa aaaatttttt
    23461 tgtaaataga ttttacaaaa atattttttg tttgattttt tcctactctc cttgtatctt
    23521 taacattaat gagaacggaa aaaatgataa tcttattatg aaaatgagga attctcatga
    23581 gcgtctccat aagactacaa atcaaaacaa tgtacaacga cttcagccca aaagaacagg
    23641 ctatcgccga ttatatttta gaaaatccaa gcaaagtaag tcatagtcct attagcaatt
    23701 tagcaagtga actgggcatt gctgactcta ctttttttca atttacaaaa aaacttggtt
    23761 ataatggatt taaagatttt aaaatggcca tgttgatcca agaaaatgat ctatctgcta
    23821 tttctatcca cgaaaatatt cagaaaacag atactgagct cactatggcg caaaaggtgt
    23881 tcgagtctag tatttcaact ttaaatgaca caaaaaagtt gttacaacaa aaggatctaa
    23941 aaaaagcggc tactattatc aatgaatcta aacgtgtcta ttttttcggt gttggcggtt
    24001 cagagattgt agcaactgac gcttatcata aatttttgcg ttcccctatc gcaacttctc
    24061 acagcacgga ttaccatatt caattgatgg aagcttcttt attgacggaa aaagactgtg
    24121 ctgttttgat ttctcacaca gggcaatcaa aagaaacaat ccatattgct gaaaccgtaa
    24181 gaaaaactgg agcaaaaaca atcgttatta ctagccaagc taattcttcc cttgctaaac
    24241 ttggcgatgt cgtttttatt tctatttctg aagaaacaga attccgatct gaagctttag
    24301 cttcccgtat ttctcaacta agcattttgg actctctata tgtgattttg atgttttaca
    24361 acgaaaaaga atcacgtcgt tctatcgcta aagttcgaca tactatttct gaaatcaaag
    24421 aatagcacca taacagaaaa agcgaaaaat ccataaaaca aaaaaagcca ctaccaaata
    24481 tttggtagtg gcttttttgg ttctgcaagg gtggtacaga acatcaatag tcggtgggtt
    24541 agaacgtatg tcatcgcatc tttggagaag gttgtattgg tattattcga gttacttgtt
    24601 agatgcgata acaaagttag tatacagtag taaaaaattt atcttttctt ttttttatta
    24661 tttttgtttc ttattttcta aaaaattaaa aaaaattttg gtgaattaag tatttccttg
    24721 ataacactgg cttttggcct cattttaggt tatcatttgt aattttattt cacagttgat
    24781 atgattgtta tgtaaaataa aaaagtttag gatacatcga atttttgggg aaattctttt
    24841 gggggtatcc taaatctttt ctattatatt cccatatttt ctattattgg tctagacgta
    24901 tgatggtaaa ctttgtacct atctgattgc tgaatcatta attgcttatc attagccatc
    24961 tttgtttcaa gttcgctaga tgatcatttt attcacatcc taacaaactt gtcacattta
    25021 tagattctta atctatttat catacaatta taaaaaagga ggtgctgctt atgaaagcta
    25081 tccatatttc tttgcttttt ttcgctagtg cattgatttt atttgctatt catctttgtt
    25141 cccagcctta tatcaataac aacggaatat tagtggaacc agcattcttt tgcttaccac
    25201 tcggcttttt gtcccttctt gcctccgcag gcttcggatt tgttgctttt tttaaacgat
    25261 caaaacaaca aatctgactt ataaaattta cgtaaaagag atcgtgacaa taatcgtgtg
    25321 atattcccaa atttcgaata aaaggtgccc cttgccatct ctaactcctc agtcacaagt
    25381 tacaatattc ataaaccatg aaaagcttgt ttattgaaat tgctccttgt gacttggagc
    25441 taagtgacag attacaacct cttttgcatc ataagacttt actccttgaa aaacattact
    25501 aacttttgat atgtttcttg cgcttgttta ttgctgaaat caaactgata ttcatggttc
    25561 aaaacagcat ttttctccgc ataatctact tttgtgactt tcacccccgc tttctcaaat
    25621 acagaagtta cttcttccat ttgtttcgta aacgtatttg tatttccatc cgttaagaat
    25681 gtgttttgcg tccaacgatc cgcatgtctg accaaggcta aacttttgac cttctctgat
    25741 tgttcaaagt ccttattatc aaagtaagcc catccacatt tttcataaag aaagctagag
    25801 attttatcac ttacttgagt aagtgcagaa ggatccaaca gaccagacaa agaaataaac
    25861 ttcgtaatct gcttgttctc caaagtcgga tcaagatgat cagctttttt aatttcttca
    25921 ttttgaagtg ctaacaaata ttctccacta atttgtgcac ctgccgaatt tccaccaatc
    25981 gaaatattcg accaatctgc ttgatctttt atctcttttt tagctgcttg aatggcttcg
    26041 ttcaattgga taactggtgt aggatgtttt gcttccggtg atcgttcata atctatattt
    26101 acgatgattt gctgcgtgtc tgaacataga tattccaaat aatcacgaca atcattttta
    26161 tcccctccta catatgcgcc tccatgcatc cagaaaagaa tcggtaattt tttcttttcc
    26221 actttcggtg tgtagatatc catattatta tttttgtact ttgaaggata ggatatttct
    26281 ttttttatat cgatttgttc gattttttgc tggaaatcat ctggtcgggc atatacttgt
    26341 tctttttcac ttgtgtcaaa ttgttttcta acaagcaagc taatcggctt tggtgtgatt
    26401 tgattaatca aaaatacagt gactaaaata actacgatga taactggtac tgcaatcact
    26461 attcgcttga accatttcat tcatttaccc tctctttctt cgccaaatcc atgtgataat
    26521 cgtgctaaca gataaaaatc cacctgaata aatgagaagc gaatgtttct tttctccagt
    26581 tttcggcaac attctttttt ttgcatctgt ctttttttgt actttcactg tcatctgaat
    26641 cggttgcgta tcatcacttg attgatcctc tcgaggttca ttctttccct tattgtctgc
    26701 attctctgtc ggatcttgct tttttggtgg agctactggg atttttttgt aatagaaaat
    26761 aatagttggt atcgtctcat taaaaatccc ttcagtcggt ccttcaacat ttgctaattc
    26821 gtatccttcg aatgtctttt gctctgtcat ataattttct ccataagctc cgtaaagctg
    26881 gatttcgtcc tggtctttga gtagactttt attgttttca tccacatact ttacgtttat
    26941 ctgatcatgg aacactactt tcaaaaagtc cgttacatgt ttcgtgtagg cagcatggtc
    27001 aacttcctgt gcctttccat gccctgctcc ttctactaat aaaagatctt tataccctcc
    27061 tgataactca tataattttt gcgcgttaga cacaggtact acatcatctg ctgttccatg
    27121 gataatcaat ttaggcaatg aatctgacat tccaatcgta ggtagagcag cttttgtaac
    27181 caaatgcatc tcctgatcaa aatagtattg tttcaaaagg cccataaata aatcttcatg
    27241 ctcttgatcc ccaatgatgt ctaactgatt ttttatggca tccggtagtg cgggggctac
    27301 tgtctggctg tacgtaaaac gtagttgttc atcaattgat gcaaatccgc aattttcaat
    27361 aattcctttg acattgacag gaatatcttt cgctaataca gacatcatcg tagctgctcc
    27421 catcgaacca ccataaagta aaatcttttg agatggccgc tctcgaactt cttgatttat
    27481 ccagtaaagg acgtcatctg actcatattg tccataggtc acataatcgc ctccgctatt
    27541 accagtcgct ctggaatcaa caaataataa attatacccg gccttataaa aatcattgta
    27601 ttcttctgta acgatcccgt tattccaatt ccctcgaaat ccgccatgga tcatgactgt
    27661 tttatctgaa ccttgatcga cataatatgc atatacatcc atcattttgc catcttgttt
    27721 gtttagtaca gtttgatgaa gttgttttgt atttggatag aagcctgtgt tttctgtagg
    27781 cgttgtccca tcatctgtac ctccaagcat cgacatcaac acactgtttt ttgcaaaagc
    27841 gatatcgtac aatagataag tagcaatcag atgtttgata atctctttat ctacaccata
    27901 atcattaaca gcaagctcag tgactaataa ctctattaat tttgacttga tgtcttctgt
    27961 tggtccatta agtatttctg tcatctgttt accgaagtaa ttcaaatatt ctttaccatt
    28021 gaaattttca cctttaccga tatcatcatt ttctgtccca ttctctttgt cacttacttt
    28081 agcagaagag gattcgtcgt ttgacatttt tgtagctgtt gcttcttctg tttgttggtt
    28141 atctgtggta gcggtcgttt cttgatctgt gctagtaaca gatgtctcaa ccgttgtttc
    28201 agtatttgtt tctgtttgtg aagtactcga tttcgtttca tttccagcat cagtagtttc
    28261 tgtggtttct acctcccgag attccgattc ggaagaaatg ctctctccat atactgtcca
    28321 ttgcgggacg cttgtactta cagcaaataa gatgaccaac gctcccttta atacttgttt
    28381 tgttctcaag ctattcactc ctttttatgt ttctaggaga attatatgta aacgcaaaca
    28441 ttttttatac aaaaaataaa tggtttatcc cataaaaata gaacaggatt attttctcat
    28501 tttacgtaat aaagcagaat ggataaacca gtatccattc tgcttttttt aaacgaagaa
    28561 acaacttatt gctccaaaaa tgtcagaaat cgtttatccc aaattttctc gtaaaaagcg
    28621 attgtttcct ttgctttcat aaaaggatta tcataagttg tcgttgctcc tctacacatc
    28681 ataagctggt atcctaatcc atgagcaaac ttcacagtcg tatctataca atactctgct
    28741 tgtgccccac ataattcgat cgaatgacag tccaattcag ttaataaaac actcaaatta
    28801 gtcttataaa atgaattcgc atgggttttt ctcacaaaat aactgtgctc aggaatagat
    28861 aattccgaca atagctccca tggttttgtt tgtggcacta attgagcatc ttcgtgttga
    28921 acgaaaataa ctggtttccc aatcgtttcg tatgttttga ttcttttatt cactaatgct
    28981 gtcactgttt ctaattcgtg tatcggcgaa gaatcaaaac ataccccttt ttgtaaatcg
    29041 atcactacta ttgcgtcggc tgtgtttgac atacttgtgc cttctttctt ttttcttgat
    29101 ttaatagtat ttcttatcta gctagttcca ttatacgtga caacattttc ttattttaca
    29161 cctctaaatg gcacttttcg actatttctt catgttatta tttcataaat acttagctat
    29221 tggaggaatg tatgatgatt aaattgtata caggttctgg ctgctcgtct tcaagacgcg
    29281 caaaacgttg gcttatcgtc catgatatac cgtttatcga acagaactta attcgtcaac
    29341 cactaaccaa aaatgaattt atccacttac taaaattgac aaacaacggc gtgaccgaaa
    29401 tgctctctac tcattctatt agttataaaa atcttgttca cagaatagat gaactcagtt
    29461 tgagtgaact atatattgag attcgtacat ccccttcttt attgaaaaaa ccgattatct
    29521 acgaccaatc tgcaaaactt ttgactggat ttagcgaaga taaaatcagg atgttccttt
    29581 ctaaaactaa aagaaagcaa gagctcaacg aactcttgct ttctggtcat cttgtcgatt
    29641 ttaaagaaaa taatcttttt gcaatgtctt aattttggca taaacaatct gcttttctta
    29701 attaacgaaa tccaattttt ccaatatgga tctatttttc tcattcctga tttttatgat
    29761 ttcagctttc agacgcgccc aatcattttt tgtaggttgt ccattccagt aaaatatctg
    29821 tgtattcgtt tgatcatatg cctttttgtc gataggataa tcgctaatga caatatcagg
    29881 taactgatca gcggatgcac tatattttat tgggcgttca gtcatagata atatcctttt
    29941 ctttgcccat aattcctgtg ttttaccata ctttgattga atatagaata tgacaggttg
    30001 ttcgtgtttt ataaaaatat ccctgactaa catacagtat tgaaaaatca accgtctgtt
    30061 cttttcataa taagtattaa gctctggaat tctgcgagct accccactaa aaaactgttg
    30121 catgattgga tacatcactg gaaatatatg ctgaaaatct ctgtcttctg ctgattttcc
    30181 aaaagcatcc accttctttt ttgtcccaaa aacccttgaa tacaaatgga tcattgccaa
    30241 attgacatat agataatttt tttctgaagt tgtaaaacaa atcttgaagt ttttttcaaa
    30301 attttctaca atagacgatt caatccagcc ccaactttct tgtactttat ctggagtatc
    30361 caacaaatct tcttccaaat aggtgttcat tacaccaaac ataaaataaa ggtaagcttg
    30421 ctctgttgga gtgatgtgat tcatcgacca gaagtttgct tcaaaaaaag gctctagtat
    30481 gtaatgcgta aactcttctt ttgttatcaa tggatgcaca aatttgttca tctcttctgg
    30541 gacatacgta atcgagtggc cttgtttcat tcgttgcaac gaaattctta gtaggctgat
    30601 ccatttcttt ctatctatac tacgcgtata tggcgcatgt ttcgcaaatt tttctaatag
    30661 ttgacgttta ttttttatac ttgataatat atcagaatga ttttcgtcga atgactgcca
    30721 atacatcaag tgaaaaaagt atcggatacg gtattcttct ccttctatcg gattaccttt
    30781 tttcaagctg caagataaat gatagttttt caaaatagta ttaagttctc ttaattttct
    30841 ttgaatcgta cgcaaactta tataattttt tgactaagat tttctaagga ttgataagta
    30901 cctttgtaaa gtgaatccag taaaatataa ccaatcgttc cctctaaaag tatagaataa
    30961 actttactca gtaaaaactg agaagaaaac tcgactttta tcatcttgtt ttctgaaaga
    31021 aaagataatt tatattctgg atacctttcg caaaacgatt ctgcttgttg aatcaccgaa
    31081 ttcatcttag ccagggtgta gtttagatct aaagcgatat ccttcaccac atataatttc
    31141 tcgtctcttt cataacaata gttcaaaaat tgatatactt gcctttcatt gatatcaata
    31201 aattcttcaa acaaagtaca tactcctttt tatctattat ttagacttat tccgacagct
    31261 acatgaataa cgtacaaata gattcgttac tgatttttca gagatactac catgcgcagt
    31321 gtgcattgtc aattattctt gctcttattt tccatttgtg ttcctgttat ttcctgtttc
    31381 tcaaatatgt tagtaaacaa aaatagatct ggcaaagcta caacttcgcc aggcaataca
    31441 gcactctcga atgaaatatt cactcaaaaa tctatcgttt tattcaattt tagatctgtt
    31501 gcttttattc tttatttcac tcgatggaag agagaacgaa aatagcttgc tcctacaatc
    31561 aaaatgagta acacacccat ccatgtaatt agcttgttca aggttctact accagtttct
    31621 ggtaaatcgc tttttctatt atctgtttct ttcgcttttt cttgatgatc aattccacga
    31681 gaaacaaaat caggctgttt tagcacactt tttaggttgc tccctgcatt tctcttttgt
    31741 tccttagttg gtatgatagg taccaaatca agttcattat ttacttttcc tgtgctgctt
    31801 tcgctgctcg tgcttcctgt gctactctca ctactcatgc ttcctgtgct actttcacta
    31861 ctcgtactct ccgtactgct ttcgctgctc gtgcttcctg tgctactttc actactcgta
    31921 ctctccgtac tgctttcgct gctcgtgttt tctgtgctaa tttcactgct cgtactctct
    31981 gtactgcttt caccgctcgt gttttctgta ctgctttcac tacttgtgct atccgtactg
    32041 cttggcggtc caacaactgt ccaactgctt ggtttcaaat tctccgtgat cgtcatctgt
    32101 ccatcataaa aaacaatcaa atcaacttta tctttactct tgatctcatc aatcgatagt
    32161 tctgcagaaa tcattattgt atactcaata ccagcggtcc attgtacgtc ttttaaataa
    32221 acttccccaa taactttttc tgaacttcca ttaccatccc aaccatatgt tagaacagca
    32281 tgtacatcac tagcattcac gcttttgttt ggtttgactg gaatagtaat gacttgttgt
    32341 gtcatgtcat aaacaatccg atcagttttc acagtaaatg aaagattctc atttgcccgt
    32401 tcatttgcag aagtactgat tccaatcatt gctgcaatta tactcagccc aagaataaca
    32461 aataatacgg tcgctgtttt cttcatttct atcactctcc atataattac cttctatttg
    32521 ataaaggaat tatataaaat gaaaatgatg agaacacgac agtcagttgt gaaaatctaa
    32581 caaaagatgt cgtgtattcg cttgaatttg tttacttttt cgtttaatca atgacagact
    32641 taaaaaagca agaccgatat cagccttgct ttcttcatct tttattgata aacagcagtg
    32701 tatttcttca tccgctttgt tttatatgca tagagtaaca aaccaacaat caagactaac
    32761 aagaataatg tagttgatat agatgcttca gccccaaagt ttcccccagt aattattgta
    32821 ttttgcgatt ggatcagagt ggagaaaaaa ccttgttctt gcgttccgct gactggtata
    32881 cccaaaatag ctccttgtgt ccaattccaa gtaatatgag caccaatagc cgtaaataaa
    32941 cttcctgtac tgtagataag tgcgctgaaa aagcaacctg ctaaaaagat attgaaaact
    33001 gccagaaatg tcatgttagg attcaatccg tgcaaaagag caaataatgc agcagatcca
    33061 atcattgcta ttaacggacc aaatctaagc atctgatgct gaaaaaagcc acggaataaa
    33121 agctcttctg atgcccccac tgcaataaat aaaataaaac tcgttatcag cggcacaatg
    33181 tttgcgtttc ctgaatctaa ccgatacgcc cccgtcaaat ataatggact agtaataagc
    33241 agaaacactc caattccaac aacgagacct cccgctaaat acccccagtc ttttcctcga
    33301 acagaaacac cataatcttc tattcttttt ccttcgacct tactagcaag aaataaagct
    33361 agtaaaaaaa cagctaaatg tccgaccgta ccggcaattg ctccaatcca ttcactattt
    33421 ccaataaaca tatccgcaag tgcattattg acaagaattc ctaaaacaaa accaacaaag
    33481 actattaaaa cagatccaat caaccgaata atcctgatca tctttcatcc ctcctttata
    33541 ttaagtttaa caaccctttt ctttcttgtc tcgtgaaaac tatgtaaatg ttctccatca
    33601 tagagagaat agatttttac gaactaagaa tatctacagc aaccttacga aaatgaaaga
    33661 ctgtctccct aattcttctg ttagactgtt tttaataagg aaaggatggg aaattttttg
    33721 aaaaaacaac tactaattat gtgcctttca gcatgtgcaa ttttactatt ctcaggttgt
    33781 agcaagtcca atgaaacgat tgcaacgttt aaaggtggaa cacttacagt tcaggatttt
    33841 tatgataacc caaaagttaa atcaaataat caaacactat taaaaaaaat gattgtcttc
    33901 aaagcatttg atgatgtata cggaaaagag atttcttcaa aggaagtaac aaaagaatat
    33961 aatgaacaaa tcaaaaaact cggtccaaac tacaaggaac aattaaaagc agttggccaa
    34021 acagaagaga catataagaa actctttaaa cagatgctgg ctttccaata cggtttgaaa
    34081 gccaatgtaa aattaacaga taaagatcta gacactgctt ggaaagaatt ctatccagaa
    34141 gttagtacac agatcattct attttcgact aaagaagaag cagataaagc aaaaaaagaa
    34201 gcaaatgaag gagaaaattt ctccaagctt gttcaagctt atggaaaaaa taaaacatta
    34261 aaggaaactg atggaaaaat gaattttgat tcaaccaatc cagagattcc tactgaagta
    34321 aaaaaagcag catttaaact aaaaaacggg gaagtttctg atatcattcc tgtaacagat
    34381 ccaacgactt atcagcaaag ctattatcta gtaaaaatgg taaaaaaaca ggataaaggt
    34441 tctaataaag acaaatacaa atctgagcta gaaaaaattg ctaccgaagc aaaaactctt
    34501 gatacggaat tcatggataa aacaatccgt aaagtgatga aaaaagacaa tgtaactatt
    34561 aaagatcctt atgtaaaaaa tatattcaaa taaacaaatt tgacccaatt ggggatgtgg
    34621 caaaatttga gaagtgattt ttggaaattc cttcttattt ttgaggcaaa ccacttttgc
    34681 cacaacttca tcggggcctt tttttatttg taccaaaaca aatcactgga tttgttacaa
    34741 tcatgaggat ataatttaaa aaagtttttt atttctatca catgaatcaa tgcgccatta
    34801 agtagattct ttcatgcttc cctatttttt cacttgccgt atcgcttcaa aaacaactat
    34861 cataaactca aactcgactg aaaagttatt ttaaaaaaga ctaaaaatat cccttctgga
    34921 cttgttcttc ttattctgtt atactagaga aacacgaaag atatttttat gcgacacttt
    34981 tcatttgttt ttatgaaatt tattcaaaac tggcgtaact tcagagttac aaggatgtat
    35041 tagaggttag ggaatacatc gtttaaatga gaaataagta atagttagta atagtcaccc
    35101 aaactttaaa ttaatgaata ttgaatcgtc agatagcctc ctcgagtttt cgagaaggct
    35161 atcgtttatt ttatggagaa attgattcag aagtaatcag cgaccttttt tagttcacca
    35221 atcgtttctg cttggaaagt ttgcacccaa tcttcttgga agcccctcac tgctttatca
    35281 atcgagggca tctctaatac ttgatctagt agctctttac ctaacgttat cgctgagctc
    35341 cagatcgaat ggctgtagtg acttgttcac aatttttgaa actagctgct aatatttttg
    35401 ttggtctttg cgtacgttcg atctcagtcc gcaacgaatc aatcactttc atagcatcaa
    35461 tgccttgatt ttgcatcctg ttataataag gtgcaatata atctgctcca ttattaatag
    35521 ctaaataccc ttgtaattct gtgtaaatag ctgtaccggt aatcaaataa ccttcttttt
    35581 tcaacttttt gatcgcttga aggcctgctt gattcaccgg caccttgata aacgtatctt
    35641 ttcccagttc ttctatgata actcgagcat cctctatcat ttcttcttcc gttcttccta
    35701 ttgtctgaat atggatagga atctgtccga ttattttttt gatttctttc atatgcgaga
    35761 agaagtttga cgctccttct tttttcagaa ttgtaggatt tgttgttacg cctgttaaat
    35821 tcaaaaactg ttgacaagaa taaatacttt ttgtatttgc tgtatcaagc atgatttcca
    35881 tatagctttc ccccattctg atcctcttga tcgattttaa tgattgtccc ctttcttaaa
    35941 tgcggcattt ccttatcttc tacatacatt gttcctggaa gttccggctc atgagatcca
    36001 tcaaatttta tcgtgatatg tcctaattca ttcaaattag tttggacaac atctcctact
    36061 gctgtgatta catacatttg tttatcaaaa cagagcttca tatcacaagt tatttctttt
    36121 cttacctgat tcaattcaat cttataacaa taatctgtta atgtttccgg tgcttcatct
    36181 ccaaataaaa tcattatttt ttctttcctg aatacttctg cttctatacc aacatctgtt
    36241 acaaccgttt caaagatcgt catctctgtt cctcctcgta aaatatcatt tgtctaatag
    36301 tttttattca aaatgtttct agattataaa atttattgaa taacacattc ctaacggaat
    36361 ttgtttcgat attgtgtgat ttctcagagt acaatggatg tatgga
//
LOCUS       DASHHB010000027        34662 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.027, whole genome shotgun sequence.
ACCESSION   DASHHB010000027 DASHHB010000000
VERSION     DASHHB010000027.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 34662)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 34662)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..34662
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.027"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(313..2205)
                     /locus_tag="U2K71_001465"
     CDS             complement(313..2205)
                     /locus_tag="U2K71_001465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315551.1"
                     /translation="MSKTNTRPSHGPAGGPGHGGPGRNLGVKGEKPKNFWGTVRRLFG
                     YMSKRMVAIIAVFVLAIAAVIFQIQTPKVLGKATTEIFKGVMAGAQQMQQGQQVTKFP
                     IDFDKIAQIIATVIVMYLISAVFNFLQQFIMTRVSQRTVYELRHDLEAKMNRVPISYY
                     DTHTNGDIMSRAINDMDNIASTLQQNLTQFVTSAVTLIGVIVMMLSISWQLTLVALLM
                     IPLSLIIVMIVAPKSQVFFADQQKSLGLLNNQVEETYGGHTIVKTFNHTEKDQEVFEK
                     ENQNLYAAGWKAQFISAIIMPLMNFVKNLGYVFVAVLGGVKVANGNMTLGDVQAFLQY
                     TTQFSQPITQLASLMNTIQSTVASAERVFEVLDEEEMSNEKSGLPEEKDTPYKVRFEN
                     VQFGYTPDNLLMTDFNLDVKPGEMVAIVGPTGAGKTTLINLLERFYDVSGGSIRYDGV
                     DTRDLSRDELRAQFSMVLQDTWLFTGSIYDNIKYGNDDATDEQIIEAAKAAHVDDFVR
                     KLPEGYNTVLNEDASNISQGQRQLITIARAFLANPDVLILDEATSSVDTRTEILIQKA
                     MNRLLENRTSFVVAHRLSTIRDADNIIVMNHGSIVETGNHDTLMEKDGFYADLYNSQF
                     SEEEAS"
     gene            complement(2202..3929)
                     /locus_tag="U2K71_001466"
     CDS             complement(2202..3929)
                     /locus_tag="U2K71_001466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315552.1"
                     /translation="MLKIVKRISLTSAIAAAVFMVIQVLADLYLPTLTSNIIDKGVAQ
                     GDVDYIWHTGFVMIGFSLISILAAIANTFFATRESQKLGKQLRTDVYKKSESLTKDAF
                     DKYGTASLITRTTNDVTQIQMVTQMFLRMMINAPITLIGASILAYQKDHQLTKIFLVV
                     IPVMIILIGGIMYFAVPLFKSMQKKTDRLNLVFREGLTGVRVIRAFDKTRFEENRFDL
                     ANKDYTNTAIKVNTIVALMMPMMTLIMSGTNVAITWFGGHYIADMTLEVGNLIAFMTY
                     AMQILISFMMLSAIFIMVPRAQASADRINEVLDEKIGIHDPENPKTVSFAGKNATLAF
                     NHVNYRYHGAEKLALEDIDFQAKSGEIVAIIGGTGSGKTTLVNLIPRLYDIESGSIQI
                     NGTDISDMTQYNLRELMGFVPQKAVLFSGTIRDNMQYGKPDATDEMIWKALEIAQAKD
                     FVSEMEDGLDSHVEQGGGNFSGGQRQRLAIARALVKTADIYVFDDSFSALDFKTDANL
                     RQALKTNMKESITVLVAQRVSTVMDADMILVLDEGKLVGKGTHEELLATNETYQEIVH
                     SQLREEDLA"
     gene            complement(3945..4595)
                     /locus_tag="U2K71_001467"
     CDS             complement(3945..4595)
                     /locus_tag="U2K71_001467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HEM7315553.1"
                     /translation="MPTQTFFHLPKEKQKRLIEAARIEFSRVPLKEASIANIVKLADI
                     PRGSFYQYFEDKEDLYFYYFETMRRDSSKDMLQLMKESDGDLFKGFESYFSKMIYEIL
                     EGENASFYRNLFMNMDYRASRKVAPHTSRQKTPHDPHKEHKQDAKELLELIDRTKLKV
                     KNDHELELLIQLVMNTVFTTIAHSYRLFSTSEDYSVEQAISEFNLKLSWLKNGVYK"
     gene            4799..5095
                     /locus_tag="U2K71_001468"
     CDS             4799..5095
                     /locus_tag="U2K71_001468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HEM7315554.1"
                     /translation="MAQSISVQDFNKLPLDADSIVLDTRNTLDYTKNHLSHSLSIPAA
                     ILPRQLKNLDPKKLYYILSYTGRRSEALADQLTANGFRAVYIIGGMEALQSSVA"
     gene            5287..5907
                     /locus_tag="U2K71_001469"
     CDS             5287..5907
                     /locus_tag="U2K71_001469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-dependent NADH-azoreductase"
                     /protein_id="HEM7315555.1"
                     /translation="MASVLVVKGHPLTAEESRTVKALTSFLTSYKENHPDDEVTVLEL
                     YRDDIPEIDEELLSGWEALRAGAEFTSLSESQQQKIARFNELTDQFLAADKIVIANAL
                     WNLNIPTKLKAWFDTVNVAGKTFRYTENGPEGLVTGKKALHIQSNGGVYNGQDFASQY
                     VKGILNFVGIDQVDQLFIEGIDYDPDRADELMQNALDKAAALGKSF"
     gene            complement(5999..7165)
                     /locus_tag="U2K71_001470"
     CDS             complement(5999..7165)
                     /locus_tag="U2K71_001470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM7315556.1"
                     /translation="MEGKNKKNRPVSWFWRWFLNNQVVTALLVVLLILLIVFLFTKVS
                     YLFAPIWQFLAIVGLPIILAGILYYLMNPVVDYFEKRKVPRLYSIIGLFVLVVALIAW
                     GSVVIIPKIQEQTVSFISNFPQYVDTIDNKLTEILRDPLFNQFREQLETAGDKFMNSA
                     GDMIQDISKSTVQSLGSFVGAVATILVALLTMPFILFYLLKDGKQLAPYFVKFLPTRM
                     QKPTLNVLSEMNSQVSSYIRGQLTVAFAVAIMFMIGFAVIGLEYAVTLGIIAGFLNLI
                     PYLGSFLAMIPAIFLAIVAGPFMIIKVLIVFAVEQTIEGRLISPLVLGNQLAIHPVTI
                     LLVLLTSGKLFGIIGVILGIPVYAAAKVVITHVFEWYTTISSLYEENPLKKQKE"
     gene            complement(7177..7530)
                     /locus_tag="U2K71_001471"
     CDS             complement(7177..7530)
                     /locus_tag="U2K71_001471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIA"
                     /protein_id="HEM7315557.1"
                     /translation="MIQAKITEIGTEAINKEEPMLILFDKTATAALRKYSVIQEVSKD
                     EPFSLKAGGTIAFDDQVYTIDYVGPMANGNLKTVAHVSLIFDACPEEGQIANGIYLHP
                     HRLPAIGLGSQIFYN"
     gene            7658..8680
                     /locus_tag="U2K71_001472"
     CDS             7658..8680
                     /locus_tag="U2K71_001472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HEM7315558.1"
                     /translation="MIEKIILGTYTRRESKGIYTIDLDTEKEELANLSLAVEENSPTY
                     VAKSKAGNLYTVTSVDGLGGAGAYDKDFHFLNAVTESGAPLCYVAVDEARQLLYGANY
                     HKGEVNVYHILGDGELKAADSIFHQEATGPHKNQDHAHVHYTDLTPDQRLVVCDLGTD
                     RVYTYDVSENGKLNLVTTFTAEPGTGPRHLVFHPNGQFAYLFGELDSTVRVLSYNKET
                     GAFEELQKISTLPEEFDGENGGAAIRISNDGKFLYASNRGHNSIAVFAVFEDGSHIQN
                     KQIISTEGDFPRDFALDPSNNFVVCANQNTDNLTLYRRHPETGLLELLKKDVYVPECV
                     CVYFDR"
     gene            8699..8860
                     /locus_tag="U2K71_001473"
     CDS             8699..8860
                     /locus_tag="U2K71_001473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315559.1"
                     /translation="MNQQDVLDRLREELNIPFFDGKIEDKEYSEEEYQKLKADLQNYF
                     EQYVQNVEN"
     gene            complement(9119..9553)
                     /locus_tag="U2K71_001474"
     CDS             complement(9119..9553)
                     /locus_tag="U2K71_001474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315560.1"
                     /translation="MLPNIGYYVQSIDQIVKETEAIGEGMHPKYEVIRNAIDEGKTSE
                     LDKSTLEEIILLFEEGTKKYRAMLMKVSTLRPPAKAMGIHKNFEKAYLSYVAGCEEMI
                     QSIDLESGVDEDLFNTSEAKQDQATDQLTAAIQKMTNLLLKK"
     gene            complement(9663..11522)
                     /locus_tag="U2K71_001475"
     CDS             complement(9663..11522)
                     /locus_tag="U2K71_001475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM7315561.1"
                     /translation="MKRYISRLSTKQKKILKGTAAIISIFFLVVFSNLFLQWCQNDLS
                     VDLALKFAFSWHTEKFFLACLVLLIILIFLIALAGSVPLGSLTYVVAIGVLGFANYMK
                     MSYRQEPIYPDDLKMITEIGLLKDMTGTMLFTVILAAAGTVIGLFCWYMFRSLKKGRR
                     FQLIRLTTLLVAIGLLGYISNFNNPDNLLRKAYNKTALWIPYSQKMNYYNTGFIGGFL
                     YNLKVEPMDEPEGYSKEKIKEITEKYQKLADEKNKTVEEESPNIVFVMSESFSDPSRL
                     NGVEVSGEPLADYYEVADQTYSGNMLSQNYGGGTANIEFEALTGFSMALFNAQLTTPY
                     TMLVPKMDQLPSIVSALNAQSYQTTAIHPYNTSMYKREDVYQTLGFDQFISERTMTYT
                     DTIENNPYISDESAYKEVMTLLKEEKTPQFIHLVTMQTHMPYDGKYDNLDYPAKTSDG
                     SGTLDLENYLQDISYSSTALKQFTEELKNLSRRTLVVFWGDHLPGIYSDTIQAKNDKQ
                     TLHETQFLMFDSKGELEKQTTHDAITSPFYFAANLMEQTNQTTNGFYQLLLSLEQELP
                     AFERELYYQNGQWYKEAQFNRSQQEIYDEYQLIQYDIVAGKQYSLADGFFEHE"
     gene            complement(11640..12800)
                     /locus_tag="U2K71_001476"
     CDS             complement(11640..12800)
                     /locus_tag="U2K71_001476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-3-phosphoglycerate dehydrogenase"
                     /protein_id="HEM7315562.1"
                     /translation="MYHVHLIDAIPEQEIEKLQGEYFKIIDDLPAEGIVIRSTKVKDE
                     WLTPDLLAISRAGVGVNTINVEKASENGTIVMNTPGVNANAVKELVLCCLLLSSRPII
                     EASRMVQTLTGPNILEQAENKRSAYVGRELQGKTIGLLGLGAIGTKVALSCYSLGMDV
                     LGYSIRDAQLDYVRQADLETVLSTSDYIVVMLPLTEDTKGLIDQANIEKMKKDAVLIN
                     VGRSEIVDKYAVMQALEKQHLAKYLTDFPEEEFLENDRILMLPHLGGSTQEALADSGR
                     LAVEALKDYLLFGTVREAVNYPSARMFFQAPFRFTIFYQKKTNILAEIFSLLNENELA
                     IADISRNHKNEHVYILIDIDSTDFEQLNQVKQQLEKISGVRKVRLLKKPERP"
     gene            complement(13107..14531)
                     /gene="araA"
                     /locus_tag="U2K71_001477"
     CDS             complement(13107..14531)
                     /gene="araA"
                     /locus_tag="U2K71_001477"
                     /EC_number="5.3.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinose isomerase"
                     /protein_id="HEM7315563.1"
                     /translation="MLNIGEKEFWFVVGSQHLYGEEALREVKKHAQEMVDELNENGQL
                     PYPIRLQELAVTADTITKIMKEVNYREEVAGVITWMHTFSPAKMWIRGTKLLQKPLLH
                     LATQYNESIPWKTIDMDFMNLNQSAHGDREYGFINARLNKQNKIVVGYWKRPEIQKEI
                     ADWMDVAVAYNESFGIKVARFGDNMRNVGVTEGDKVEAQIQFGWTVDYFGIGDLVQVI
                     DRVSDEEVDQLFEEYKELYTFDYGDYEEKTWEEHVKVQAQQEIGIRRFLEEGGYNAFT
                     TNFEDLYGMKQLPGLAVQRLMAEGYGFAGEGDWKTAAIDRLLKIMARGKDTGFMEDYT
                     YELASGQEAILESHMMEVDPTLAATKPRIVVSPLSMGDREDPARLVFDGKAGEGVVVS
                     MADFGTHYKLLINEVEAFEPTTEAPNLPVARVLWKTKPNFHEGVHSWIQAGGGHHTVV
                     SLNLTTDQIETWAKLVELETVVIR"
     gene            complement(14547..15245)
                     /locus_tag="U2K71_001478"
     CDS             complement(14547..15245)
                     /locus_tag="U2K71_001478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005873905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEM7315564.1"
                     /translation="MLEQLKEEVYQANLDLPKHGLVKYTWGNVSAFDPETRYFVIKPS
                     GVSYEEMTADDMVVVDLDNHIIEGKLNPSSDTPTHAVLYRSFPQIGGIVHTHSTWATI
                     WAQAGLDVPAMGTTHADTFYGSVPCARFLTQQEIDSGYEYETGKVIIETFKERKIDPL
                     AIPGVLLHGHGPFTWGKDAQSAVMNAVVLDEVCKMNLFTRQLNSFSEELPQRILDKHY
                     LRKHGKNAYYGQNK"
     gene            complement(15202..16863)
                     /locus_tag="U2K71_001479"
     CDS             complement(15202..16863)
                     /locus_tag="U2K71_001479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HEM7315565.1"
                     /translation="MKEVNAQSMLDVIDGRTSLGIEFGSTRIKAVLIDSSFTPIAAGS
                     YEWENKLENGIWTYSLEDIWKGLRVSYRKMAAEVFEIYGENLTTIGSIGFSAMMHGYL
                     AFDSKEQLLVPFRTWRNTITEEAERILTEKFQYNIPQRWSIAHLYQSILNKEEHVKEI
                     NFLTTLAGYVHWQLTGEKVLGVGDASGMFPIDPSTKTYHQEMKNIFGQLIEKAHVAID
                     LDHILPKVLVAGEEAGKLSESGAYLIDPTGNLRPGIPVCPPEGDAGTGMVATNSVAKG
                     TGNISAGTSAFAMIVLEKELENVHPEIDMVTTPSGELVGMVHTNNCSSDINAWVSIFE
                     EFTNSLGMEISRDKLFTVLFNKALEGDTDCGGLLSYGYFSGENITGVNEGRPLFVRTP
                     GSRFDLANFMRIHLASAFGAMRIGMDILKSENVQIDRLVGHGGIFKTPEVGQRILASA
                     MEAPVTVMDTAGEGGAWGIALLAAYMMDRHGKSLENFLAEDVFGQDQGITITPSAEEI
                     AGYQIFMRRYREGIDIEKTAIHQLQEKGVIENAGTTERRSIPSKS"
     gene            17081..18481
                     /locus_tag="U2K71_001480"
     CDS             17081..18481
                     /locus_tag="U2K71_001480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar porter family MFS transporter"
                     /protein_id="HEM7315566.1"
                     /translation="MKKEKKVSSSFIYFFGAFGGILFGYDIGVMTGALPFLQHDWGLA
                     GKASLIGWITSSVMLGAILGGSLSGLLSDKLGRRKMILLSALIFMAGSVLSASAPHNG
                     SYFLIAARILLGLAVGAASALVPAYMSEMAPARLRGRLSGINQVMIASGMLLSYVADY
                     LLKGLPETMAWRVMLGLAAVPALILFFGVLALPESPRFLMQSGRLEEAKRVLNYIRTP
                     KEAEQEFEQIQLNVKQEKTTGTSWHTLFLEKYRSLVIAGIGVAVFQQFQGANAIFYYI
                     PLIVEKATGHAASDALMWPIIQGIILVAGSLLFLVIADKFNRRTLLKIGGSVMGLSFI
                     LPAVLGTVLDAHTNSLLILLFLCIYVAFYSCTWAPLTWVIVGEIFPLAVRGRASGLAS
                     SFNWIGSFLVGLLFPVMTASMSQEIVFGIFGIICFLGVLFIQEIVPETRGKSLEEIEQ
                     SASKKTYPKRISIHHS"
     gene            complement(18733..19812)
                     /locus_tag="U2K71_001481"
     CDS             complement(18733..19812)
                     /locus_tag="U2K71_001481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM7315567.1"
                     /translation="MAKYQDIAEEIKQKILREEYEVNTTIPSEFKLQEIYDVSRHTIR
                     QAIAVLVNEGYLRKEKGSGTYVDDSYKKEKTPLNRTKTIGVITTYLSDYIFPSIIRGI
                     ENELRESGYSLLLASTNNDHELEKECLKKMIAYGVDGLIVEPTKSNQYNPNLALYVAL
                     REQGIPMVMINAAYEELNASYICLDDVKVGFMAAEYLIKKGHQRLLFITKIDDLQGKY
                     RMKGFISACEQYNVRFLTEDIITFTTETRETIAEKTVQRLKEEQALDGIICYNDQIAS
                     LMLDKLTENGYHIPDDLSIVGTDDSSLSHLGNKKLTTLTHPKEKLGQDAARWIVTTIE
                     KGQAQPNILYEPVLVERETVKENEK"
     gene            complement(19898..20857)
                     /locus_tag="U2K71_001482"
     CDS             complement(19898..20857)
                     /locus_tag="U2K71_001482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 43 protein"
                     /protein_id="HEM7315568.1"
                     /translation="MMKTLYNNPIVRQRADPWVYKHTDGYYYFTGSVPGYQVIELRRA
                     KSLNELEHAASLIVWQAHEEGPMSELIWAPEVHYINGKWYIYFAASNHKTIRDESHHH
                     RMFVLENQHIDPMNTDWIEKGQIKTKRETFSLDGTTFSSQGKLYYVWAQQDPEITGNS
                     NLYLSEMDNPWTLKGKQILLSIPEYEWEKRGFSVNEGPAVLTRNGKIFITYSGSATDE
                     NYAMGLLWADEHKNLLLKESWHKLSEPVFVSSEKNKQFGPGHNSFTVSEDGCFDILIY
                     HARPEKNQKGDPLDNPNRHANAQCFTWNEQGFPEFGEPVPYAL"
     gene            complement(20869..22380)
                     /locus_tag="U2K71_001483"
     CDS             complement(20869..22380)
                     /locus_tag="U2K71_001483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEM7315569.1"
                     /translation="MKVKIREDFTVGEISSRLFGSFVEHMGSVVYTGIYEPGHETADG
                     DGFRQDVLSYVKELELGVIRYPGGNFTSGYDWVDTVGRKEQRPKKIDLAWRGIEPNSF
                     GLHEFFKWISHVQAKPIFTVNLGTKGIDEAKNLVEYCNFPKGTFWSDLRIKNGQKKPF
                     GVKLWCLGNELDGPWQVGAKSAYEYGRLANEASKVMKLVDDSIETILVGSSTPRLPSY
                     PEWDRIALEEAYENVDYLALHNYIDKYDEEDLTKPPKNERDDTATYLAKAYRFDRQIE
                     EIVATCDYVKGYLRSEKTMYLAFDEWNVHAQPEKRHRDFEVGSPIDWCYFTMEDTLLF
                     GSLGLAILRHADRVKISCQSLLVNTIPLILTDAKGQAWRNPTYYVMQHLSKYAKGQVL
                     KQQIICPVYDCEKIKNVPYVDSVIVDNGNQLTIFLINRTNEQQKITIEHDFNLLDQGE
                     HYTLTSSDLLDKNTREEPEKIKPEINYFSIEKHDELNVGIEKYSWNVIRIKKM"
     gene            complement(22402..22966)
                     /locus_tag="U2K71_001484"
                     /pseudo
     CDS             complement(22402..22966)
                     /locus_tag="U2K71_001484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="YesL family protein"
     gene            complement(22972..23835)
                     /locus_tag="U2K71_001485"
     CDS             complement(22972..23835)
                     /locus_tag="U2K71_001485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM7315570.1"
                     /translation="MAPESSITVTVNSRKAVSGKKIIRTISMLVILLVIAFPFLWLII
                     SSFKHEKDIISFPPRIFADSYTLDNYIKVWTTIPLLDYIKNTVIFAGGTVITSVFFDS
                     LAGYAFARMRFKGKSVLFYFVLLTMMIPFQVFMIPLFIEVNLLGMLDTYAGLIIPRMT
                     TAFGIFMMRSFFITLPDSLEEAARIDGLSEFNIFLKIMLPLSKPTLLSLGIFTLMNSW
                     NDLLYPLILTSSSKMRTLPAGLALFTGQNISFYGPVMAGTVISMLPLLVVYIFAQKYF
                     VQGTAMSGMKE"
     gene            complement(23849..24745)
                     /locus_tag="U2K71_001486"
     CDS             complement(23849..24745)
                     /locus_tag="U2K71_001486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7315571.1"
                     /translation="MNGFTKYWNRPDRAAYLFLTPSFLVLLLFTVIPLLGTFAISFTD
                     LNIFFTSRNFVSFENFTRIFSDERAVNSLIHSLYFTVLETPTQIIVGLLAAVALSKNT
                     RFNRFCRSTFYIPVICSLTSIAIIFSMLLDPNVGAIPYLFSQVGLPIPQFFRDPTLAM
                     PTVAIMTVWKNFGVTMTILLTAIQGISPSLYESAQMDGATNRQQFFNITLPQIVPSLG
                     FCILTNLIGSMQVFDQVYVATAGGPQFKTETAVQYIYSRGFTAPYELGYASALSSVLF
                     IIVATIAISTNVYMSRKEKQMK"
     gene            complement(24920..26209)
                     /locus_tag="U2K71_001487"
     CDS             complement(24920..26209)
                     /locus_tag="U2K71_001487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HEM7315572.1"
                     /translation="MKEEQKMKGKKWLIGSLLVAATGILGACGGGSSAESDSDKEVTL
                     TFWNGFTASDGEILQEIVNEFNKTNDKNIKIEMDIMTWANLNEKLPTAISSKKAPDFI
                     ALNYPDFAQYVENGAVQPLDDFWEYDGVDKSDFKETAIELGEVEGKQYFVPMQVQGMY
                     MYWNNDLFKQAGLDTEQPPTTWEELTEMAPKLTDSSKNVSGFVFNKDGTAPLYNWMLA
                     NGGKLVNDDYTKSEFASPENLKTLKAIQKMIHVDKTGPESISGAEMDNLMNAGQLAIE
                     INGPWLNNGLKANEIDYNVTTLPQGSDSDKKTAILDGVGYAIPASTDNNKKEAVYEFL
                     KYWNTTEIGKKWSIENGFPAYLDSVAKDEEVKNDPIVSELSKQMDYAEPYLPGFTQIS
                     AINNDIINPLIEKLLAGEDPETLMNDADDAINQLLQK"
     gene            complement(26181..27698)
                     /locus_tag="U2K71_001488"
     CDS             complement(26181..27698)
                     /locus_tag="U2K71_001488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-N-arabinofuranosidase"
                     /protein_id="HEM7315573.1"
                     /translation="MEATMLISSVKRVGKVDPRLYSSFIEHMGRAVYEGIYQPDHPSA
                     NKSGFRQDVIHLVKELNIPMIRYPGGNFVSGFRWEDSIGPIANRPRRLDLAWRTIETN
                     EVGIHEFYNWCQHVGAEINMAVNLGTRGVDAARNLVEYCNFPKGTYWSDLRRSNGQES
                     PFGIKTWCLGNEMDGPWQIGHKTAEEYGRLAEETAKAMKLVDDSIEVVLCGSSNRQMP
                     TFGDWELTVLDQAYDQIDYLSLHQYYGNQKNDLSHYLARSLDMDRFISEVTAMCDTVK
                     AKKHSKKQINLSFDEWNIWYHSAEQDEQAAPWQIAPPLLEDNYNFEDALLLGCLLITL
                     LKHADRVKIACLAQLVNVIAPIRTETNGEAWRQTIYYPFMQAAVYGQGEVLSPQIRSA
                     NYATEDFEEVPYLESIAVINDKELVIFAVNRGEEEMNLTVHLSELSLEGVIDFSEMSG
                     FDTKATNLPKSEQVKPSHSTNVKAEDGKINCLLKPLSWNVVRCRLSDEGGTKNER"
     gene            27896..28786
                     /locus_tag="U2K71_001489"
     CDS             27896..28786
                     /locus_tag="U2K71_001489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArsR family transcriptional regulator"
                     /protein_id="HEM7315574.1"
                     /translation="MQLQINEESLPVYEALASKTRIKIIQLLSKKKMNVKDLAKELGV
                     SSAITTMHVKKLEEANIIKTEKVGQQKISSLRVDKIDISFPEKIFNAFDTKETSIPIG
                     HYTNYAIEPTCGLATIHDFIGKVDEPRYFMDPRRMDARILWFTSGFVEYQAPNFLNTE
                     DTLEMLEVSVEISSEFPFSNDNWPSDITFSLNGVELGTWTSPGDFADIRSKYTPDWYP
                     DNLNQYGLLKTIRITKHLTNMNGEPLSNITINDIPKEQDTWHLRIEVKDDAKHVGGCT
                     LFGKGFGNYDQDIKLKVYYS"
     gene            complement(28859..29683)
                     /gene="nadE"
                     /locus_tag="U2K71_001490"
     CDS             complement(28859..29683)
                     /gene="nadE"
                     /locus_tag="U2K71_001490"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonia-dependent NAD(+) synthetase"
                     /protein_id="HEM7315575.1"
                     /translation="MSLQTEIINELGVKPTIDPKEEIRKSIDFMKAYLKKYPFLKTFV
                     LGISGGQDSTLAGRLAQLTMEEMREETKDENYQFIAVRLPYGEQADEEDAKAALDFIQ
                     PDVSLRVNIKPAVDAQVQVLSEAGVGISDFNKGNIKARQRMITQYAVAGERAGAVLGT
                     DHAAENITGFFTKFGDGGADILPLFRLDKRQGKQLLQALNAPEKLYTKIPTADLEDGK
                     PMIADEVALGVTYNEIDDYLEGKEVPAQAQEKIEAWWNKTQHKRHLPISVLDDFWK"
     gene            complement(29695..31167)
                     /locus_tag="U2K71_001491"
     CDS             complement(29695..31167)
                     /locus_tag="U2K71_001491"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HEM7315576.1"
                     /translation="MNNTYPDDSLTLHTDLYQINMMQTYWELGRADLHAVFECYFREM
                     PFNHGYAVFAGLERLVNYLENLTFNDSDIAYLRGLEVYPEGFLEYLQNFEFKATVRSA
                     REGELVFANEPLIQVEGPLAHCQLVETALLNMVNFQTLIATKAARIKSVIGEDPLLEF
                     GTRRAQELDAAVWGTRAAYIGGADATSNVRAGKIFGIPASGTHAHSLVQSYGNDYEAF
                     MAYAKTHKDCVFLVDTYDTLKSGVPSAIRVAKELGDKINFQGVRIDSGDMAYISKRVR
                     EQLDAAGFTEAKIYASNDLDEATILNLKMQKAKIDVWGVGTKLITAYDQPALGAVFKL
                     VSIEDDEGKMMDTIKLSSNAEKVTTPGKKQVWRITRNFDGKSEGDYVTLWDEDPREEE
                     AIFMFHPVHTFINKTVRDFTARPVLQDIFIEGKRVYELPALNEIKEYTKENLDSLWEE
                     YKRDLNPQKYPVDLSTECWNHKMAIMERMKKSVAELHTEA"
     gene            complement(31804..32166)
                     /locus_tag="U2K71_001492"
     CDS             complement(31804..32166)
                     /locus_tag="U2K71_001492"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1827 family protein"
                     /protein_id="HEM7315577.1"
                     /translation="MKLIETPVNSNLNIKTFYPKVVEFFFGNTAINYYKLFSLDRTQL
                     LLVDTYDKKQVVMINTKKKITRQEIDYAIHHVLKMTREDVKVHVGVKQELERAGIQFK
                     RPNKDIVVIEQKNTKDSI"
     gene            32389..33141
                     /locus_tag="U2K71_001493"
     CDS             32389..33141
                     /locus_tag="U2K71_001493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM7315578.1"
                     /translation="MEYTIKKMSEISGVSPRTLRFYDEIGLLKPARINSSGYRIYGKK
                     EVDRLQHILFYRTMAFKLDDIQEVLDNPSFDHQKALIKHQQMLLEKRAQIDTLLTTVQ
                     QTLDMYEGGRKMSDKEKFEGFKQQKLKENEESYGQEIRQKYGKKKVTASNEKWMNMDK
                     ETYEEMQTVEKQMLFDLTLYLKEPEDESLADRIFQAHKKWFSYSWPEYQPKAHKGLGM
                     MYEADERFTAYYDERSGHGAAKALNEIIQKKA"
     gene            complement(33281..34204)
                     /locus_tag="U2K71_001494"
     CDS             complement(33281..34204)
                     /locus_tag="U2K71_001494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7315579.1"
                     /translation="MANIRDIAKLTGYSVSTISRVINGHPYVDEEKRKEILAVMKEVD
                     YIPNANARQLSYGKTKSIGVILPYTNHPYFDQLISGIIEAAFDYDYKVTLLPTGYHKE
                     KERRYLEEFAAKAFDGLIITSRANTLDDLLAYQKYGPLVFCEEIKGKQASCVFIDREQ
                     SITEGLTYLKQQGIKQLGITLGRSGRLSYNSKITLQLCHQLFPSFDESLVFWDCIDAE
                     KGKQAGFFFKELGVDGVFTNSDMVAAGILQSYLQDKTPVVIGRDNLLISELLGFPTID
                     HHLEACGEMAFQLFLTEKKDKVKIPYTFIQR"
     gene            complement(34219..34491)
                     /locus_tag="U2K71_001495"
     CDS             complement(34219..34491)
                     /locus_tag="U2K71_001495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315580.1"
                     /translation="MNTYVEITDGTTNNYFYAFVEFKGSVLTLYSFQGLNRNIVKEIP
                     MNEIENLTKDIYWGGQRISFSHEGKMYQFFECGPAVVDYLQENLFV"
BASE COUNT        10232 a         7213 c         5978 g        11239 t
ORIGIN      
        1 caagaggttg tgagcctgcc gtttaactcc gagtcacaag gagcaatttc aaaaaacagc
       61 ttctcaggtt tgttgactat tgtgacttgt gaccgaggag ttggcaggag aacaccgttt
      121 aaggaagagg ttgtaaaatc agcggtttga cctgagtatt agagatacaa aacggaaaaa
      181 tagcttctca tattttgccg ttttttgagc taataccgaa ggacagctga ttgaacaccg
      241 tttattcgga aaaagggact gtgacacgac ttttgtcaca gcccctaaaa aaaataataa
      301 atttgtacta ttctatgaag cttcttcttc agaaaactga ctgttataaa gatctgcata
      361 gaatccgtct ttttccatca atgtatcgtg gtttcctgtt tctacgatcg aaccatgatt
      421 catcacaata atgttgtctg catcgcgtat tgtagacaag cggtgagcaa caacgaaact
      481 tgtccggttt tccaataggc ggttcatcgc tttttgaatc agtatttctg ttcgtgtatc
      541 tacactagat gttgcttcat caaggatcag gacatctgga tttgctaaga atgcccgcgc
      601 aattgtgatt agctgacgct gcccttgcga aatattgctt gcatcctcgt ttaatacagt
      661 gttatatcct tctggcaatt tacgaacaaa atcgtctacg tgagcagctt tagcagcctc
      721 aatgatttgt tcgtctgttg catcgtcgtt cccatatttg atattgtcat agatacttcc
      781 tgtaaacagc caagtatctt gcagtaccat tgaaaactgt gcgcgcaatt catcacgtga
      841 aagatcacgg gtatctacac catcgtaacg aatactacct ccagaaacat cgtagaaccg
      901 ttcaagaagg ttgatcagtg tcgtcttccc tgcaccagtc ggacctacaa tagcgaccat
      961 ctcaccaggt ttgacatcca agttaaaatc agtcattagc aagttatctg gtgtgtagcc
     1021 gaattgaaca ttttcaaaac gaactttgta tggtgtatct ttttcttctg gaaggccaga
     1081 tttttcattg gacatctctt cttcatccaa cacttcaaat acccgttcgg ctgaagcaac
     1141 tgttgattgg atagtgttca tcaaacttgc aagttgagtg attggttgag agaactgtgt
     1201 tgtatattgc aagaacgctt gaacatcccc taatgtcatg ttcccatttg caacttttac
     1261 tccgcctagt acagcgacga acacatagcc tagattttta acgaaattca tcaacggcat
     1321 gatgattgcc gaaataaatt gagctttcca accagcggca tacagattct gattttcttt
     1381 ttcaaatact tcctggtctt tttcagtatg attgaatgtt ttaacaatgg tatgaccacc
     1441 atatgtttct tctacttggt tattcaataa acctaaactt ttttgctgat ctgcgaaaaa
     1501 cacttgtgat ttcggcgcta cgatcatcac gatgatcaag cttaaaggga tcatcaataa
     1561 cgcgactaac gtcaattgcc agctgattga aagcatcatc acgatgaccc caatcaatgt
     1621 gactgcacta gttacaaatt gagtcaaatt ttgctgcagc gtgcttgcga tgttatccat
     1681 atcattgatt gctcgagaca tgatatcccc atttgtatgt gtatcataat aggaaatcgg
     1741 tacacgattc attttggctt ccaaatcatg acgtaattca taaactgttc gttgggaaac
     1801 acgtgtcata ataaattgct gtaaaaagtt gaatacagca gaaatcaaat acatcacgat
     1861 tactgtcgca atgatctgcg cgattttatc aaagtctatc ggaaattttg ttacttgttg
     1921 gccttgttgc atctgttgag cgccagccat tacacctttg aatatttctg tggttgcttt
     1981 ccccaagacc tttggtgttt ggatttggaa gatcacggct gctattgcta gtacaaatac
     2041 agcgatgatc gcaaccatac gtttagacat atagccaaac aatcggcgta ccgttcccca
     2101 gaaattttta ggcttttcac cttttacacc gagatttctg ccaggtccac catgtcctgg
     2161 accgcctgct ggtccatgag agggacgtgt gtttgtttta ctcatgctaa gtcctcctct
     2221 cttagttgtg aatgaacgat ttcttgataa gtttcgtttg ttgctaatag ttcttcatgt
     2281 gttcctttgc caaccagttt tccttcatct agcacaagga tcatatctgc atccattact
     2341 gtacttactc gttgagcaac taaaacggtg atactttctt tcatattcgt tttcaatgct
     2401 tggcgaagat tggcatccgt tttgaaatcc aacgctgaga aggaatcatc aaacacatag
     2461 atatctgctg ttttgactaa tgcccgtgca attgccagac gctgtctttg tccaccagag
     2521 aagtttcctc ctccttgttc cacatggcta tctaagccat cttccatctc tgaaacaaaa
     2581 tctttagctt gtgcgatttc taatgctttc caaatcattt catctgttgc atccggctta
     2641 ccatactgca tattgtctcg gatcgttcca gagaacagaa ctgctttttg aggaacaaag
     2701 cccattaatt ctcgcaaatt atattgagtc atgtcactaa tgtccgttcc atttatttgg
     2761 atcgaaccag attcgatgtc atataatcga gggatcaaat tgactaaggt cgttttacct
     2821 gaaccagtcc cccctatgat tgcgacgatc tcgcccgact tggcttggaa atcaatatct
     2881 tccaatgcaa gtttttctgc tccatgatat cgataattga catgattgaa ggccaatgtt
     2941 gcgttttttc cagcaaaaga aactgtttta ggattttcag ggtcatggat accgattttt
     3001 tcatccaaca cttcattaat acggtcagct gaagcttgtg cacgaggcac catgataaaa
     3061 atagcggaaa gcatcataaa gctgatcaag atctgcatcg cataagtcat aaatgcaatc
     3121 agattaccga cttccaaagt catatctgct atgtagtgtc caccaaacca agtgattgct
     3181 acgtttgtac cgctcatgat caatgtcatc attggcatca tcaatgcgac gatcgtatta
     3241 actttgattg ccgtatttgt ataatcttta ttcgctaaat caaaacgatt ttcttcaaag
     3301 cgtgtcttat cgaatgctcg aatcactctg acccctgtca atccttctcg aaataccagg
     3361 tttaaacggt cagttttctt ttgcatactt ttgaataatg gaacagcaaa gtacatgatt
     3421 ccgccgatca aaataatcat tactgggatg acaactaaga agatttttgt caactgatga
     3481 tctttttgat aagcaagtat gctcgcgcca atcaacgtga ttggtgcatt gatcatcata
     3541 cgtaaaaaca tttgtgtaac catttggatt tgagtcacat cattggtcgt tcttgtgatc
     3601 aaagaggccg ttccatattt atcaaacgca tcttttgtca gactctctga ttttttatag
     3661 acatccgtac gcaattgctt acccagtttc tgtgattcac gagtagcaaa aaatgtattt
     3721 gcgatagcag ctaagatact gatcaaagaa aagccgatca tcacaaaacc tgtatgccaa
     3781 atataatcga catctccttg cgctacccct ttatcaataa tattggaggt aagtgtcggc
     3841 aaatataaat ctgccagtac ttggatcacc atgaaaactg cagctgcaat tgctgaagtc
     3901 aaggagatcc gttttacgat ttttagcatc tagttccctt ctttctattt atatactccg
     3961 tttttcagcc aagaaagttt caaattgaat tcactgattg cttgctctac tgagtaatct
     4021 tcagatgttg aaaataagcg atacgaatgc gcaattgttg taaatacagt attcatcact
     4081 aattggatca gtaattccaa ttcatgatca ttttttactt tcagcttcgt ccgatcgatc
     4141 aattcaagca actcttttgc atcttgtttg tgttctttat gtggatcgtg aggcgttttt
     4201 tggcgactcg tatgaggagc gacttttctt gaggcacggt aatccatgtt cataaacaag
     4261 ttacgataaa aagaagcatt ctcgccttcc aaaatctcat aaatcatttt agaaaaatag
     4321 gattcgaatc ctttgaatag atcaccatcg gattctttca ttagttgtaa catatcttta
     4381 gagctgtcac gtctcattgt ctcaaagtaa taaaaataaa gatcctcttt atcttcaaaa
     4441 tactgataga aactgcctct agggatatcc gctaatttga cgatattagc aatcgaagct
     4501 tcttttaagg gtactctaga aaactcgatt ctcgcagcct cgattagtct tttttgtttt
     4561 tctttaggca aatggaaaaa tgtctgtgtc ggcatatgga ttctcctttc tttcaagatg
     4621 acacattgtc atatgacgct atgtcattat aacacctcct acctgttttg caagacattt
     4681 gcatagtttt tttcattatc atccgataca ttaaaaaagt gataagagga atccttccat
     4741 ttgaattaat gagagttgcc cattaaaata gccacatact aaacaaagga tgagataaat
     4801 ggcccaatca attagtgtcc aagacttcaa taaattgcct ctcgatgctg acagcatcgt
     4861 tttagataca agaaatacct tagattacac aaaaaaccac ttaagccact cgctttctat
     4921 ccccgctgct atcttgccaa gacaattaaa aaatctagat ccaaagaaac tttattatat
     4981 tctaagctat acaggcaggc gatcagaagc attagccgat caacttactg ctaatggttt
     5041 tcgtgctgtt tatatcattg gtgggatgga agctcttcag tcgagtgttg cttgaatatt
     5101 gcttgttaga ttcaaaagag gctgtgacaa aaaatcgtgt cacagcctct tttataccct
     5161 gtcttttttt agttatttat tcttcaacct tatcaatatg acttgatagc cttttctttt
     5221 tcgagtaaac tgattttgtt agtcatagtt ataacaatca tagcaataaa aggagaaggt
     5281 aagcatatgg catctgtttt agtagtcaaa ggtcatcccc taactgcaga agaatcgcgc
     5341 acagttaagg cattgacgtc ttttttaaca agttataaag aaaatcatcc agatgatgaa
     5401 gtgactgttt tggaacttta tcgcgatgat attccggaaa tcgatgaaga attattatca
     5461 ggttgggaag cattacgtgc tggtgcagaa ttcacttcct tatcagaaag tcaacaacaa
     5521 aaaattgctc gtttcaatga attgactgat cagttccttg ctgctgataa aatcgttatc
     5581 gcaaacgcct tatggaatct aaatatccca acaaaattaa aagcttggtt cgataccgtc
     5641 aatgtagctg gaaaaacgtt ccgctataca gaaaacgggc cagaaggatt agtgacaggt
     5701 aaaaaagcac tgcacatcca atctaatggc ggtgtataca acggacaaga ctttgcctct
     5761 caatatgtaa aaggtatttt aaactttgta ggcatcgatc aagtagatca gctattcatc
     5821 gaaggaatcg attatgaccc tgaccgtgca gatgagttga tgcaaaatgc attagataaa
     5881 gctgctgctt taggtaaatc attttaaaaa ttgagataaa tgcatacaaa agagacagag
     5941 gaaaaaatct tcaaagcagc atttgtgcct tggatatttt cccatctgtc tctttttgtt
     6001 attctttctg ctttttcaac ggattttctt cataaagaga agagattgtt gtgtaccatt
     6061 caaaaacatg agtaatcact actttagctg ctgcataaac aggaatcccc aagatcacac
     6121 cgatgattcc gaacaatttc ccagaagtca gcagaactaa taaaatcgtt actggatgga
     6181 ttgctaactg attccctagt actaatggag agatcaagcg tccttctatt gtctgctcta
     6241 ctgcaaaaac gatcagtact ttgatgatca tgaatggacc tgctacaatg gctaaaaaga
     6301 tagccggaat catcgctaaa aatgatccta agtaagggat caggtttaaa aaacccgcaa
     6361 taatccccaa cgtgacagca tactctaaac caatgaccgc aaaaccaatc ataaacatga
     6421 ttgccacagc aaaagccaca gttagttgtc ctcttatata agaagaaact tgactattca
     6481 tttcactcag cacattcaac gtcggttttt gcatgcgtgt cggtaaaaat ttgacaaaat
     6541 aaggtgctaa ttgtttaccg tctttcaata gataaaataa gataaacggc atcgtcagca
     6601 gagccactaa aatagtcgct accgcgccga caaaacttcc aagactctga actgtactct
     6661 tagatatatc ttggatcata tcgcctgccg aattcatgaa cttatcacct gctgtttcca
     6721 actgttcgcg aaattggttg aacagcgggt cccgcaaaat ctcggttaat ttattatcaa
     6781 tcgtatctac atattgaggg aagttactga tgaagctcac tgtttgttcc tgaatcttag
     6841 ggatgatcac tacgctcccc catgcgatta gtgcaacaac caaaacaaac agcccaatga
     6901 tactgtaaag ccttggtact tttcgctttt caaagtaatc aactaccgga ttcatcaagt
     6961 aatacaagat tcctgctaaa atgataggca gacctacgat tgctaaaaat tgccagattg
     7021 gtgcaaacaa atatgaaact tttgtaaata aaaagacgat cagcaatatt aacaatacaa
     7081 caagtaaggc tgtgactact tgattattta aaaaccagcg ccaaaaccac gataccggtc
     7141 tgttcttctt attttttcct tccataattc ctcctgttag ttataaaaaa tttggcttcc
     7201 caaaccaata gcaggcagtc tatgcggatg aagatagatt ccatttgcaa tttgtccttc
     7261 ttctggacaa gcatcaaaaa tcaatgatac gtgagctact gtctttaaat tcccattggc
     7321 catcggaccg acataatcga tcgtatatac ctgatcatca aaagcaattg ttccgcctgc
     7381 tttcaaagaa aaaggttcgt cttttgatac ttcttgaatc accgaatatt ttcgtaaggc
     7441 agctgttgct gttttatcga acaaaatcag catgggttct tccttgttta ttgcttctgt
     7501 cccaatttct gtaatctttg cctgaatcat tgcactctct cctttaattg ctttcaaaaa
     7561 cagtataaca cattctcatt ctttagccgt gtggaataat tatgtgatag aataaaaacg
     7621 aaaactattt cattacaatt aggaggaatc agctcatatg atcgaaaaga taattttagg
     7681 aacctatacg cgtcgcgaaa gtaaaggaat ctatacgatc gaccttgata cagaaaaaga
     7741 agaattagcc aacctttcat tagctgttga agaaaatagc cctacctatg tagcaaaaag
     7801 taaagctggc aatctttaca cagttactag tgtggatgga ttaggtggtg ccggtgcgta
     7861 cgataaggat tttcattttt tgaatgcggt aacagagtct ggcgctcctt tgtgttatgt
     7921 agcggttgat gaagcacgtc aattactata cggggcgaac tatcataaag gtgaagtaaa
     7981 tgtctaccat atattaggag atggcgaact gaaagcagca gattcaatat tccatcaaga
     8041 agcgacaggt cctcataaaa accaagatca tgcgcatgtc cactatacgg atctgactcc
     8101 tgaccaacga ttagtcgtat gtgatcttgg tacagatcgt gtgtatactt atgatgtgtc
     8161 cgaaaacggc aaattgaatc ttgtaactac cttcactgca gagccaggaa caggccctcg
     8221 ccatcttgtc tttcatccaa atggacaatt cgcttattta tttggtgaat tagacagcac
     8281 agtccgcgtg ctttcttata ataaagaaac aggtgctttt gaagaattac agaaaatatc
     8341 gaccttgcca gaagaattcg acggtgaaaa tggaggggca gctatccgca tttcaaatga
     8401 tggcaagttc ctttatgctt ctaaccgagg acataactcg attgctgtat tcgcagtttt
     8461 tgaagatggt tcccacattc aaaacaagca aatcatctca acagaagggg attttccccg
     8521 cgatttcgca ctagatccaa gcaataattt tgtcgtgtgt gccaatcaga acaccgataa
     8581 tctgacatta tatcgccgtc atcctgaaac aggattactg gaattactga aaaaagatgt
     8641 ctatgtacca gaatgtgtct gtgtttattt tgatcgttag taagaaaagg agaatgaaat
     8701 gaatcagcaa gacgtattag acagactacg ggaagaacta aatattcctt tctttgatgg
     8761 gaaaatcgaa gataaagagt atagcgaaga ggaatatcag aaactcaaag ccgatttaca
     8821 gaactacttt gaacaatatg tacagaatgt agaaaactaa tcagctttcc atactatctg
     8881 aaaagaacat caggacaaga cttttccgtc tatcctgatg ttcttttcta caaataaaaa
     8941 accgccgaga tggttagact cgacggaaaa ggagtattta ctcgattttg agtaagtaca
     9001 aggttgttgt tggttatagg tatgtcatta tcataaagga ttttgatcac tctatcataa
     9061 aggtaatatt acatttatat tagataactc tagtttttat tgttatcaaa gatttctttt
     9121 attttttcaa caacaaatta gtcatttttt ggatggcagc tgtcaattgg tctgtcgctt
     9181 ggtcttgttt tgcttcagat gtattgaata gatcttcatc tacccctgat tcaagatcga
     9241 tactttggat catttcttca cagcctgcta catatgaaag ataagctttt tcaaaatttt
     9301 tatgaatgcc cattgctttt gctggtggac gcaaagtact gactttcata agcattgccc
     9361 gatatttttt tgttccttct tcaaataaca ggatgatctc ttcaagagtc gatttgtcca
     9421 gttcacttgt ttttccttca tcaattgcgt ttctgatcac ttcatatttc ggatgcattc
     9481 cttctccgat tgcttctgtt tccttgacga tttgatcgat ggactgcaca taatagccaa
     9541 tattcggtaa catgttcgtt ttcctacttt ctccattgtt gttattattt attctacttg
     9601 tttttatcct caaatgacaa gtacttgaaa aaatttaatg gtttctatac agctcgccat
     9661 tcttattcgt gctcaaagaa accatctgct aaactatatt gtttccctgc gacaatgtca
     9721 tattggatca attgatattc gtcgtaaatc tcttgttgcg agcgattgaa ttgcgcttct
     9781 ttataccact gtccgttttg atagtacagt tctcgttcaa acgccggaag ttcctgttct
     9841 aatgataaaa gcagctgata aaagccattt gttgtctgat tcgtctgttc cattagattc
     9901 gctgcaaaat aaaacggact ggtgattgca tcgtgagtcg tttgcttttc taattctcct
     9961 ttactgtcga acataagaaa ttgtgtttca tgcaatgttt gtttgtcatt ttttgcttgg
    10021 atcgtatctg aatagatccc tggaagatga tccccccaaa aaacaactaa agtccttcta
    10081 gaaagattct tcaattcttc cgtgaactgc ttcagtgccg tgctgctata ggaaatatct
    10141 tgcagataat tctctaagtc aagtgtccca cttccatctg aagtttttgc aggatagtct
    10201 agattatcat actttccatc gtagggcata tgtgtctgca tcgtgactaa atgaatgaac
    10261 tgaggtgttt tttcttcctt gagaagtgtc attacttctt tgtaagctga ctcatcagaa
    10321 atatatggat tattctctat cgtatccgta taggtcatcg tacgctcgct gatgaactgg
    10381 tcaaatccta atgtctgata aacatcctct cttttataca tcgaagtatt gtatggatgg
    10441 atcgctgtcg tctggtatga ttgtgcgttt aacgctgaaa cgatagaagg aagctgatcc
    10501 atttttggta caagcatcgt gtaaggggtg gtgagttgcg cattaaataa cgccatagaa
    10561 aaaccagtca atgcttcaaa ttcgatattt gctgttccac cgccatagtt ttgagaaagc
    10621 atattgccac tatatgtttg atcagctact tcataataat ctgccaatgg ctcaccagat
    10681 acttctacgc cgtttaatcg actaggatct gagaaacttt cactcatcac gaacacgatg
    10741 ttcggcgact cttcctctac agttttattt ttttcatcgg ctagcttctg gtatttttca
    10801 gtgatttctt tgatcttttc tttcgaatat ccttcaggtt catccattgg ttctaccttc
    10861 agattgtaca aaaacccacc gataaaacca gtgttatagt agttcatttt ctggctgtaa
    10921 ggtatccata gtgccgtttt attatatgct tttcgcaaca aattatcggg attgttgaaa
    10981 ttactgatat accctagcag tccaattgcc acaagcagtg tcgttaatcg gatcaattgg
    11041 aatcgccttc cttttttcag gcttcgaaac atgtaccagc aaaacaatcc tatcacggtt
    11101 cctgcggcag ctaagatcac tgtaaacagc atagtgccgg tcatatcttt cagcaatccg
    11161 atttcagtga tcatcttcaa atcgtctgga taaatcggtt cttgccgata actcattttc
    11221 atataatttg caaaacctaa tacaccgatg gctacgacat acgtaaggga acctaaaggg
    11281 acagatcctg ctagtgcaat cagaaaaata agaataatca gcaagacgag acaggctaag
    11341 aagaattttt ccgtatgcca agaaaaagca aatttcaacg caaggtctac ggatagatca
    11401 ttttgacacc actgcaaaaa taaattacta aaaacaacaa gaaaaaagat actgatgatt
    11461 gctgctgttc cttttaaaat ctttttctgt ttggtcgaaa gtctgcttat atatcttttc
    11521 aaatgcttgc tccttcattt ctatcaataa aagaatgttg cttctcttat tttagttgta
    11581 cgaaattcga aagtaaagaa cggaaagaag aattaatgaa atccaaatat tctctttctt
    11641 tatggccttt ctggtttctt taataagcgg acctttcgta ctccagaaat tttttctagc
    11701 tgttgtttca cttgattgag ctgttcaaaa tcagtactgt ctatgtcaat caatatgtag
    11761 acatgctcat tcttatggtt acgcgatata tctgcgatcg ctagctcgtt ctcattcagc
    11821 agagaaaata tctctgctaa tatattggtt ttcttttgat aaaatatagt gaatcgaaac
    11881 ggcgcttgga aaaacattcg tgcggatgga taattgaccg cttctctaac cgtaccgaac
    11941 aaaagataat cttttaaggc ttcaactgcc aaacgtccgc tgtctgccaa agcttcctga
    12001 gtcgatcccc ctagatgagg caacataagt atacggtcat tttctaagaa ctcttcttct
    12061 ggaaaatcag tcaagtattt agccagatgt tgtttttcca atgcttgcat aacagcatat
    12121 ttatcaacaa tttcacttct tcctacattg ataagtacag catctttttt cattttttct
    12181 atattcgcct gatcgatcaa tccttttgta tcttctgtca acggcagcat gactacgatg
    12241 taatccgagg tagaaagaac tgtttctaaa tctgcttgcc gaacataatc caactgggca
    12301 tcgcggatag aataacccaa gacatccatc cctaagctat agcaacttag cgctactttt
    12361 gtgcctatcg ctccaagacc aagaagaccg atggttttgc cttgcaattc tcgtcccaca
    12421 taagcacttc ttttgttttc tgcctgttct aaaatattag gacctgttag tgtttgtacc
    12481 atacgacttg cttcgattat cggacgggaa gataagagca aacagcacaa aacaagctct
    12541 tttactgcat tagcatttac acctggtgta ttcatgacga tcgtcccgtt ttcagaagct
    12601 ttttcaacgt tgatcgtgtt caccccaaca ccagctcttg aaattgctag caaatctggt
    12661 gtaagccatt catctttcac tttcgtacta cgaataacta ttccttcggc tggcaaatca
    12721 tctattattt tgaaatattc cccttgtaac ttttctatct cttgttctgg aatagcatca
    12781 atcaaatgaa cgtgatacat aaaaccccct ccttactaac aagtatctcg aaatttctgc
    12841 aattagacaa ctgtttacca tttgacttcc tagaagcaaa aagagcagtt agagcagagt
    12901 aggctgagtg ataaaaataa ggctatgaca agttactgcc ataagcctta ttttcgagag
    12961 caaaatattg agggtgagat atcttgataa atggtgttcc gttagctgac attgataata
    13021 agactaaatt tcaaaaaagt tgaaaagcaa atttcgtcaa tctcttctta tttcttaaaa
    13081 ctaagtggct ttttcacaac ctctttttat ctaataacca ctgtttccaa ctcaactaat
    13141 tttgcccacg tttcaatttg atctgttgtt agatttagcg agacaacggt atgatgaccg
    13201 ccacctgcct gaatccatga atggacacct tcatggaaat tcggtttagt tttccataac
    13261 acacgtgcaa caggtaaatt aggtgcttca gtcgttggtt caaaggcttc tacttcattg
    13321 atcagcagtt tataatgcgt accgaaatct gccatcgata cgactacccc ttctcctgct
    13381 ttcccatcaa atacaagacg cgccggatct tctctatctc ccattgataa aggagaaaca
    13441 acgattcttg gtttcgtcgc agcaagcgtc ggatctactt ccatcatatg ggattccaag
    13501 attgcttctt ggccactagc tagctcgtaa gtataatctt ccatgaatcc tgtatctttc
    13561 cctcgagcca tgattttcag caaccgatca atcgctgcag ttttccagtc gccttcccca
    13621 gcaaaaccgt atccttctgc catcaaacgt tgtacagcta gtccaggtag ttgtttcatt
    13681 ccatacaggt cttcaaaatt agtcgtaaaa gcgttatagc ctccttcttc taagaaacgg
    13741 cgaataccga tttcctgctg agcttgtact tttacatgtt cctcccatgt cttttcttca
    13801 taatcaccat agtcgaaggt atacaattcc ttgtactctt caaataattg atcgacttct
    13861 tcgtctgata cgcgatcgat tacttgtaca agatctccga taccaaaata atcaacagtc
    13921 caaccaaatt gaatctgtgc ttccactttg tctccttctg tgacacccac gtttctcatg
    13981 ttgtcaccaa aacgagccac ttttattcca aaactttcat tataggctac tgcaacatcc
    14041 atccaatcag caatctcttt ttggatctca ggtcgtttcc agtagccaac gacgatttta
    14101 ttttgtttat ttaatcgagc attgatgaac ccatattcgc ggtcaccatg agcggattga
    14161 tttaaattca tgaaatccat atcgatcgtt ttccaaggaa tgctttcgtt atattgcgta
    14221 gctaaatgaa gcaatggttt ttgtagaagc tttgtcccgc ggatccacat ttttgctggt
    14281 gagaaagtat gcatccaagt gatcacacct gctacttctt ctcgataatt gacctctttc
    14341 atgatttttg tgatcgtatc agcagtaact gctaattctt gaaggcggat tggataaggc
    14401 aattggccat tttcattcag ttcatcgacc atttcttgtg catgtttctt tacttctcgc
    14461 agtgcttctt ccccatataa atgctgtgat cctacaacaa accaaaattc tttttctcca
    14521 atattcaaca taattagcca ctccttttat ttgttttgtc cgtaatacgc attttttccg
    14581 tgttttcgta aataatgttt atctaaaatc cgttgaggca gctcttctga aaatgaattg
    14641 agctgtctgg taaataaatt catcttgcat acttcatcta gtacgactgc gttcattacc
    14701 gcactttgcg catctttccc ccaagtaaat ggaccgtgtc cgtgtaaaag tacccctgga
    14761 attgctaagg gatcaatttt gcgttctttg aatgtttcta tgataacttt tcctgtttca
    14821 tattcgtacc cgctgtcgat ttcctgttgt gtcaaaaatc gagcacaagg taccgatcca
    14881 taaaaagtat ctgcatgcgt cgtccccatc gcaggaacat ccaatcctgc ttgtgcccag
    14941 atcgtcgccc atgtcgagtg agtatggacg attcctccga tttgaggaaa tgaacgatat
    15001 aaaacagcat gagtaggcgt atccgaagag ggattgagtt taccttcaat gatatgattg
    15061 tccaagtcca caacgaccat gtcgtctgct gtcatctctt cataactgac tccgctaggt
    15121 ttgataacga aatatcgtgt ttcaggatca aaagcgctca catttcccca tgtatatttg
    15181 acgagtccgt gtttaggcaa gtcaagattt gcttggtata cttcttcttt cagttgttcc
    15241 agcattttct atcactcctt tttcttgcaa ttgatgaatc gctgtttttt caatgtcgat
    15301 cccctcacgg taacgtctca taaatatctg atagcctgct atttcttctg ctgaaggtgt
    15361 gatggtgatt ccttggtctt gtccaaacac gtcttctgcc aagaagtttt ctaaagactt
    15421 cccatgtcga tccatcatat aggctgctaa taacgcgatt ccccacgctc caccttctcc
    15481 ggctgtgtcc atcactgtaa ctggtgcctc catagcagat gctagaatcc gctgtccaac
    15541 ttctggtgtt ttaaaaatcc ccccatgccc cactaaacga tcaatttgga cattctcaga
    15601 tttcaatata tccattccga ttctcattgc accaaaagca ctggctaaat ggatccgcat
    15661 gaaattcgct aaatcaaaac ggctacctgg tgtacgtaca aataatggcc ttccttcatt
    15721 tactcctgta atgttttcac cggaaaaata accataagaa agtaatcccc cgcaatccgt
    15781 atcgccttct agcgctttgt tgaataatac cgtgaataat ttatctctgc ttatctccat
    15841 acccaaagaa ttggtaaatt cttcaaaaat actgacccaa gcattgatat ccgaagagca
    15901 gttattcgta tgaaccatgc ctactagttc tcctgaaggt gtagtgacca tgtcgatttc
    15961 cggatggaca ttttctagtt ctttttctaa tacgatcata gcgaaggcgg aagtaccagc
    16021 cgaaatgttc cctgtccctt tggctacact gttcgtagcg accataccgg ttcctgcatc
    16081 cccttctggc ggacaaaccg ggattcctgg tctcaaattt cccgtagggt cgatcagata
    16141 agcccctgat tcgcttaact tgccagcttc ttctcctgct actagaactt taggcaaaat
    16201 gtgatccaag tcaatcgcta cgtgcgcttt ttcgattaac tgaccaaaaa tatttttcat
    16261 ttcttgatgg taggttttcg ttgaaggatc gataggaaac atgcctgatg catctcctac
    16321 gcctagtact ttctcaccag taagctgcca atggacataa cctgcaagcg ttgtcaaaaa
    16381 attaatttct ttcacatgtt cttctttatt caaaatagat tgatacaaat gagcgatact
    16441 ccatctctgc ggaatattgt attgaaattt ttccgttaat attcgttctg cttcttcagt
    16501 gattgtattt cgccaagtac gaaatggtac tagtaactgt tccttgctat caaatgctag
    16561 ataaccgtgc atcattgcac tgaacccaat cgacccgatt gtcgtaagat tctcgccata
    16621 gatttcgaat acttctgctg ccatttttcg gtaactgacg cgcagtcctt tccaaatatc
    16681 ttctaatgaa taggtccaga taccattttc tagtttgttt tcccactcat aactaccagc
    16741 ggcaatagga gtaaaagatg aatcaatcaa tacagctttg atcctagttg atccaaactc
    16801 aatccctaaa gatgtacgac catctatgac atctaacata ctttgcgcat ttacttcctt
    16861 cataatgatt taactccctc cttctcgtca aacctgatca aaccgtcttt ttagtagcgc
    16921 ttacaatgat tattgtatac ttttgtacgt acatgtcaag cagttattta caagaataca
    16981 ataaaaaaca cttgttttgt acgtacataa gttataaaat gaaattgctt acaaaatctg
    17041 atcaaccgcc ttctcgaaat tctagaagga gagaatactt atgaaaaaag aaaaaaaagt
    17101 ttcctcatcg tttatttatt tttttggcgc atttggcggc attctatttg gatatgatat
    17161 tggtgttatg accggcgcct tacctttttt acagcatgat tggggtttgg caggtaaagc
    17221 ttctttgata ggatggatca cgtcttcggt catgttaggt gcgatccttg gcggctcttt
    17281 atctggtctt ctttctgata agcttggacg acgaaaaatg attcttcttt ctgcacttat
    17341 ttttatggct ggttctgtcc tttctgcatc tgcccctcac aatggttctt attttctgat
    17401 tgcagcaagg attcttctag gtttagctgt tggcgctgca tctgccctag ttcctgctta
    17461 tatgtctgaa atggctcctg ctagactaag gggaagattg tcagggatca atcaagtgat
    17521 gattgcttct ggcatgctcc tttcctatgt tgcagattat ttgctaaaag ggttgcctga
    17581 aacgatggct tggcgcgtga tgttagggct cgctgctgtc cccgcgctga ttttattttt
    17641 cggtgtttta gctctaccag agtcaccgcg ttttcttatg caatctggcc gcttggaaga
    17701 ggcaaaaaga gtattgaact atattcggac accaaaagaa gctgaacagg aattcgaaca
    17761 aatccagctg aatgtcaaac aagaaaagac aaccggaact tcttggcata ctttgttttt
    17821 agaaaaatat cgttccttag tcattgccgg tattggtgta gcagtattcc agcaatttca
    17881 aggagctaat gctatttttt actatattcc gctgatcgta gaaaaagcga ctgggcatgc
    17941 agcgagcgat gcattgatgt ggccgatcat ccaaggaatc attttagttg ctggttcttt
    18001 gctctttttg gttattgcag acaagttcaa tcggcgaact ttattgaaaa taggcggtag
    18061 cgtgatggga ctttctttca tcctaccagc tgttttagga actgtactgg atgctcatac
    18121 aaattcgttg ttgattctct tgtttttatg tatttatgtc gcattttatt cttgcacatg
    18181 ggctccactg acatgggtaa tcgtaggcga aatattccca ctagctgtcc gcgggcgagc
    18241 atctggccta gcctcttctt ttaattggat tggttctttc ctagttggct tgctttttcc
    18301 tgtgatgact gcttctatgt ctcaagaaat cgttttcggc atttttggga tcatctgctt
    18361 tttaggggtt ttatttattc aagagatcgt accagaaact cgtgggaaat ctttagagga
    18421 aatcgaacaa agtgcatcta aaaaaactta tcctaaacgt atttctatcc atcattctta
    18481 aagaggttgt gacagaagtg gtcagcttca agaaataaga aggaaccgct tagtttccag
    18541 tcacaaggaa caatttcaaa aaacagcttc tcatgtttga tgaatattgt gacttgtgac
    18601 cagttggtag gagaacaccg tttagtggag aggtggtgaa agaaacgttc agccttgagc
    18661 gatgacaaaa gctctcacaa acattgcgac gagtaaccgt aggagcaata agaggcggta
    18721 aaaacccgtc tcttattttt cgttttcttt cactgtttcc cgttcaacta gtacaggttc
    18781 atagagaata tttggctgtg cctgtccttt ttcaatcgtt gtgacgatcc accgtgcggc
    18841 atcttgtccc aacttttctt ttggatgcgt caatgttgtc aattttttat tccctaaatg
    18901 gcttaaagaa gaatcgtctg ttcctacgat cgataggtca tctggaatat gatagccatt
    18961 ttctgtcaac ttatctagca ttaaactagc tatttgatcg ttatagcaaa tgatgccatc
    19021 taaagcttgt tcttctttca accgttgtac tgttttttca gcgattgttt ctcgtgtttc
    19081 cgtggtgaaa gtaatgatat cttccgtcaa aaaacgcaca ttatactgtt cacacgcgct
    19141 gataaaacct ttcatcctgt attttccttg aagatcgtcg atttttgtga tgaacaacag
    19201 acgctgatgt ccttttttta tcaaatattc tgctgccata aaaccgactt tcacatcatc
    19261 taaacaaata taagaggcat ttaactcttc ataagcagca ttgatcatca ccatcggaat
    19321 gccttgttct ctcaaagcaa cataaagtgc taagtttggg ttatactgat tacttttcgt
    19381 aggctcaacg atcaaaccat ctaccccata agcaatcatt ttcttcaaac actctttctc
    19441 caattcgtga tcgttattcg tactggctaa aagcaatgaa tacccagatt ctcgtaattc
    19501 attttcgatt cctcggataa tcgacggaaa aatataatca gagagatatg tggtgatgac
    19561 accgatcgtt tttgttctgt tcaatggtgt tttttctttc ttatagctgt catctacata
    19621 tgtgcccgac cctttttctt tacgcagata gccctcattt accaacacag cgattgcttg
    19681 ccgaatcgta tgtctgctga cgtcatatat ttcttgcagc ttgaattctg agggaatggt
    19741 tgtatttact tcgtattcct cacgaaggat cttttgtttt atctcttctg caatatcttg
    19801 atattttgcc atttgccttg ccccttttta tttgtacgta catatatcat aacgagtctg
    19861 caaggtttat tcaagcaaaa atcaaggttt cttgtgctca taaggcatac ggaacaggtt
    19921 caccaaattc tggaaatcct tgctcattcc acgtaaagca ttgcgcgttc gcgtgccgat
    19981 taggattgtc taacggatcc cctttttggt tcttctctgg tcgagcatga tagatcaaaa
    20041 tatcaaagca cccgtcttct gaaaccgtaa acgagttgtg accaggacca aattgcttat
    20101 ttttttcaga agaaacgaat actggctccg ataatttatg ccatgattct tttaataaaa
    20161 gattcttgtg ctcatcagcc cacagcaggc ccatggcata gttttcatct gttgcactac
    20221 cagaatacgt aatgaatatt ttcccattgc gagtaaggac tgctggacct tcatttactg
    20281 aaaaaccccg tttttcccat tcatactcag gtatagaaag gaggatctgc ttccccttta
    20341 acgtccaagg attatccatt tcggatagat aaagattaga attcccagta atttcaggat
    20401 cttgttgggc ccacacataa tatagctttc cttgagaaga gaaagttgtt ccgtctaaac
    20461 tgaaagtttc ccttttcgtt tttatttgtc ccttttctat ccaatctgta ttcattggat
    20521 ctatatgctg attttcaagt acgaacatgc ggtgatgatg agattcatca cgtatagttt
    20581 tatgattgct tgcagcaaag taaatatacc attttccatt gatataatgg acttcaggtg
    20641 cccagatcaa ttcactcatc ggtccttctt catgagcctg ccatacaatc aggcttgcag
    20701 catgttctaa ttcattcagt gactttgccc ttcttaattc aatcacttgg tagcctggaa
    20761 ccgatcctgt aaaataataa taaccatctg tatgtttata tacccaagga tctgcccgtt
    20821 gccgaacgat aggattatta taaagtgtct tcatcataac aaatttcctc acattttctt
    20881 tattctaatt acattccaag agtatttttc tatccctaca tttaattcgt catgtttttc
    20941 tattgaaaaa tagtttattt caggttttat tttttctggt tcttcacgtg tatttttgtc
    21001 caacagatcg ctactggtta atgtgtagtg ctctccttga tctagcaaat tgaaatcatg
    21061 ttcaatcgtt atcttttgtt gttcatttgt gcgattgatc aagaaaatag tcagctgatt
    21121 gccgttatct acgatcacac tgtccacata aggaacattt tttatctttt cacaatcata
    21181 aacagggcaa atgatttgtt gtttcaatac ttgaccttta gcatatttcg ataagtgttg
    21241 catcacataa taagttggat tcctccatgc ttgtccttta gcatctgtca gtattaaagg
    21301 tattgtgttt actaacaatg attgacaact aatctttacg cgatctgcgt gtcgtaaaat
    21361 agccagccct aatgaaccaa ataataatgt atcttccata gtaaagtaac accaatcgat
    21421 gggactaccg acttcaaaat ccctatgtct tttttccggt tgggcatgta cattccattc
    21481 atcaaacgct agatacattg ttttctcact tctcaaatat ccttttacat aatcacaagt
    21541 agcaacgatt tcttcaattt gacgatcgaa tcgataagct tttgcaaggt atgtcgctgt
    21601 atcatcacgc tcatttttag gaggtttagt caagtcttct tcatcatact tatcaatata
    21661 gttatgcaaa gctaaataat ctacattctc ataagcttct tctaatgcga tacgatccca
    21721 ttcagggtac gatggtaaac gtggcgtaga gcttccaact aagattgttt caattgaatc
    21781 atccactaac ttcatgactt ttgaggcttc atttgctaac ctaccatatt cataagcact
    21841 cttagccccc acttgccatg gaccgtctag ctcattccct aaacaccata atttaacgcc
    21901 aaaaggcttt ttttgcccat tttttattct taaatcactc cagaaggttc cttttggaaa
    21961 attacaatat tctactaaat tttttgcttc atcaattcct tttgttccta aattcaccgt
    22021 gaatatcggt ttcgcttgta catgactgat ccatttaaaa aattcgtgga gtccaaaaga
    22081 atttggttct atacctcgcc atgctaaatc aatttttttt ggtcgttgtt ctttcctacc
    22141 tacagtatcc acccaatcat aaccagatgt aaagtttcct ccaggatagc gaatgacacc
    22201 taattctaat tcctttacat aagagaggac atcttgtcta aacccgtcac catcagctgt
    22261 ttcatggccc ggttcataaa tccctgtata gacgacactt cccatatgct caacaaatga
    22321 gccaaacagc cgagaactga tctctcctac cgtaaaatcc tctctgattt ttactttcac
    22381 ttcttcgtcc aacagctttc cctacctttc atttcctata gtcaagataa tagatacaag
    22441 tttccatacg ccaccagcaa agataaaaat aaaaggaggg aaaagaaata tgagcagtac
    22501 aaatcctaat aacaatacgc aacaaagaat atttctcaaa aaatcaaaaa atactttcca
    22561 tgcactttgt tcaaaaattt gtaaaaatgt gatatctttg ttttcagaaa tcacatatat
    22621 ccaggataaa agaaacatgg aataaataag taaaactaca tatccactaa ttgaaagtac
    22681 gattgtctgt tcttctaata attgattaac aagaaaataa ataatgatcg ttaacagact
    22741 aaggaacaag ttcttaccaa aatgttgctt tattccttga aagaaaaaat aaatgatatt
    22801 tcctgatccc tcacctttcc attgcgccat tacataaaaa aacagcacat gtagcaggca
    22861 cgatagtaat aacaggcaaa cacacgatta cccagagaat acctaataag atgtaatcag
    22921 aaaccacaat caactgtcca taaagtttag tctcaactat tttcatcttc ctcactcttt
    22981 cattccagac atagctgttc cttgaacaaa atatttctgt gcaaaaatat aaaccactag
    23041 taatggcagc attgaaataa ccgttccagc cattacaggt ccataaaaag aaatgttctg
    23101 ccctgtaaat aaagctaacc ctgctggtaa agttctcatt tttgaactac ttgtcaatat
    23161 caatggataa agcaagtcat tccaactatt catcagcgtg aaaatcccta atgataaaag
    23221 agtaggtttg ctcaatggca acatgatttt caaaaaaata ttaaactcac ttaatccatc
    23281 aatgcgtgct gcctcttcca aagaatctgg taaagtaata aaaaaggaac gcatcatgaa
    23341 gataccaaat gcagttgtca tacgaggaat gatcaaacct gcataagtat caagcatgcc
    23401 aagcaaattg acttcaataa atagaggaat catgaagact tggaatggga tcatcattgt
    23461 cagcaaaacg aaatagaaca aaactgattt tcctttgaaa cgcatacgag caaatgcata
    23521 ccccgcaaga gaatcaaaga atacagaagt gatcactgtc cctccagcaa aaatcactgt
    23581 atttttgatg taatctaata aagggatcgt tgtccaaacc ttaatatagt tatctagggt
    23641 atagctatct gcaaatattc taggcggaaa tgagataatg tctttttcgt gtttaaaaga
    23701 agaaataatc agccacaaaa aaggaaacgc aataaccaac aatataacta gcatactaat
    23761 cgttcgaatg atttttttac cactaactgc ttttcgacta ttcacagtaa ctgtaatcga
    23821 gctttctggt gccatacagg tttccctcct atttcatttg tttttctttc cgtgacatgt
    23881 acacattagt ggagatagct atagttgcaa caatgataaa caaaaccgaa gacagtgctg
    23941 atgcatagcc caattcataa ggtgctgtaa atcctcgact gtaaatatat tgaacagctg
    24001 tttctgtctt aaattgtggg ccaccagcgg ttgcaacata tacttggtca aacacttgca
    24061 tcgaaccaat cagattcgtt aaaatacaaa aacctaagga gggaacgatc tgaggtagcg
    24121 taatgttaaa aaactgctgt cgatttgttg caccatccat ttgagcagat tcatacaaag
    24181 agggagagat tccctgaata gcagtcaaga gaatcgtcat agtaacacca aaattcttcc
    24241 aaacagtcat gatggctacc gtcggcatag caagggttgg atcgcgaaaa aattgaggga
    24301 tcggcaagcc gacttgtgaa aacaagtaag gaatcgcccc tacattcgga tcaagcaaca
    24361 tagaaaaaat aatcgcaatt gacgtcaatg aacaaataac tggaatgtaa aatgtcgaac
    24421 gacaaaaacg attgaatctg gtatttttag ataatgctac tgctgctaat aaaccaacga
    24481 taatttgtgt tggcgtttcg agtacggtga aatataaaga atgaatcaac gaattaactg
    24541 ctcgttcatc actaaatata cgtgtgaaat tttcaaaact tacaaaattt cttgatgtaa
    24601 agaaaatatt caaatctgta aaactaatag caaatgttcc taaaagcggt atgacagtaa
    24661 ataataataa aactaaaaaa gatggagtta aaaacaaata cgcagcacga tcaggacgat
    24721 tccaatattt ggtgaatccg ttcatatagt ccacctcatc tttcattttg ataaaatata
    24781 caatcttggt ttaaacgaat ctgaataaga tatccgaaca atcgctgtga tttttgcaat
    24841 aacacctatc tgcaaatact aggtcgatca ttcagatatc tgtatcttgc caacagtcta
    24901 tcctgtataa agttctgtac tatttttgca gtaattgatt gatggcatca tctgcatcgt
    24961 tcatgagtgt ctcaggatct tctcctgcta ataatttttc aatcagtgga ttgatgatat
    25021 cgttattgat cgcagagatt tgggtaaaac ctggtaaata tggctctgca tagtccattt
    25081 gtttcgataa ttcagataca ataggatcat tttttacttc ttcatctttt gctactgaat
    25141 ccaagtaggc aggaaaacca ttttcgatac tccatttttt accaatttca gttgtattcc
    25201 aatatttcaa aaactcatag acagcttctt ttttattatt atctgtactt gccggaatgg
    25261 catatcctac gccatctaaa atagctgttt ttttgtctga atcactccct tgtggtaagg
    25321 tagtcacatt ataatcaatc tcgtttgctt tcaatccgtt atttaaccat ggcccattga
    25381 tttcaatggc tagttgacca gcattcatta aattgtccat ttcggcgcca gaaatcgatt
    25441 ctggaccagt cttgtctaca tggatcattt tttggatcgc ttttaacgtt ttaaggttct
    25501 caggagacgc aaattcagac ttagtataat catcgttcac taatttaccg ccatttgcta
    25561 acatccagtt atataacggt gcagttccat ccttattgaa gacaaaacct gatacatttt
    25621 tactactatc agttaatttt ggtgccattt ctgtcaattc ttcccaagta gtaggtggtt
    25681 gttcagtatc taatccagct tgtttaaaca aatcattatt ccaatacata tacatgcctt
    25741 gtacctgcat ggggacaaaa tactgtttac cttctacttc acctaattca atagccgttt
    25801 ctttaaaatc agatttatct actccatcat attcccagaa atcgtctaat ggttgtaccg
    25861 ctccgttttc tacatactgt gcaaaatctg gataatttaa tgcaataaag tcaggtgctt
    25921 ttttagaaga aatcgctgta ggaagttttt cattcaaatt ggcccaagtc atgatatcca
    25981 tttcaatttt gatgttttta tcatttgttt tattaaattc attaacgatt tcttgcaata
    26041 tttcaccatc agatgcagta aacccattcc aaaaagtcaa agtcacttct ttatcagaat
    26101 cagattctgc tgaacttccg ccaccgcaag ctcctaaaat acctgttgca gctaccaaca
    26161 aagagccaat taaccatttt ttacctttca ttttttgttc ctccttcatc tgataaacga
    26221 catctgacca cattccaaga tagcggtttc aataaacaat tgattttccc atcttcagct
    26281 tttacatttg ttgaatgact tggttttact tgttcacttt taggaagatt agttgctttt
    26341 gtatcaaatc cactcatttc agaaaaatca atcactccct ctaatgataa ttcagacaag
    26401 tgaacagtta gattcatttc ttcttctcca cggttaactg caaatatgac aagttctttg
    26461 tcatttatta cggctatact ttctaaatac ggcacttctt caaaatcttc cgtcgcatag
    26521 tttgctgaac gaatttgtgg gcttaacacc tctccttgtc catacacagc agcttgcata
    26581 aatggataat agatcgtctg tctccaagct tctccgtttg tttctgtcct gattggtgca
    26641 ataacattta ccaattgagc aagacaagca atctttactc tatctgcatg tttcaataaa
    26701 gtgattaata gacagcctaa tagtaatgca tcttcaaaat tatagttatc ttctagtaaa
    26761 ggaggagcaa tctgccaagg cgctgcttgc tcatcttgtt ccgctgaatg ataccaaata
    26821 ttccattcat caaaggacag gttgatctgc ttcttactat gtttcttcgc cttgactgta
    26881 tcgcacatcg ctgtcacttc agaaatgaac cgatccatgt ctaaggaacg tgcaagataa
    26941 tgagaaagat catttttttg gttgccataa tattgatgca gagacaaata atctatttga
    27001 tcgtacgctt gatcaagaac tgttaactcc caatcaccaa atgtaggcat ttgccgattc
    27061 gaacttccgc ataaaaccac ctcaattgaa tcatctacga gtttcatcgc cttagccgtt
    27121 tcctctgcta accgtccata ttcttccgct gttttatgtc caatctgcca aggaccgtcc
    27181 atttcattcc ctaaacacca tgtcttaatt ccaaaagggc tctcttgacc attcgaacgc
    27241 cttaaatcac tccaataagt tcctttagga aaattacaat attcgactaa attacgagct
    27301 gcatctactc cacgagtacc cagattgaca gccatattga tttcggcccc cacatgctga
    27361 caccagttat aaaattcatg aatcccaact tcattcgttt caattgtccg ccaagctaaa
    27421 tccaaccttc taggtctatt tgctatgggc ccaatactgt cttcccaacg aaatcctgac
    27481 acaaaatttc caccaggata tctaatcatg ggaatgttca attcttttac caagtgaatg
    27541 acgtcttggc gaaatccact tttattagct gatggatgat ctggctgata aatcccttca
    27601 tatactgctc gtcccatatg ttcaataaat gaactgtaca accgcggatc aactttgcct
    27661 acacgcttga cactactaat caacattgtg gcttccataa ctgacctcct ttaatttata
    27721 catgaataat acacctcgtt gaaagcgttg tcaataatat aaatgttaaa cttacttatt
    27781 ttttatcgat tttaattaac caaaatatta ataatgatta aaacaaataa tttaatataa
    27841 cttttattaa caaatatgtt aatatataag aaaaaccttt taagaggtga tatatatgca
    27901 actacaaata aacgaagaat ccttaccggt atatgaagca ttagctagta aaacaaggat
    27961 caagatcatc cagctacttt ccaaaaaaaa gatgaatgtc aaagatttgg caaaagaact
    28021 tggagtgagc agtgctatca cgacgatgca cgtgaaaaag ctagaggaag caaacatcat
    28081 caaaacagaa aaagtaggac aacaaaaaat ttcgagcctt cgtgtcgata aaatcgatat
    28141 cagttttcca gaaaaaatat tcaatgcttt tgatacgaaa gaaacatcga tacctattgg
    28201 tcactataca aattatgcaa ttgaaccaac atgcggttta gctacgatcc atgattttat
    28261 cgggaaagtg gatgaaccac gctattttat ggatcctcga aggatggatg cccgcattct
    28321 ctggtttacc agcggatttg tcgagtatca ggcacctaat tttttaaata cagaggatac
    28381 attggagatg ttggaagttt ctgtggaaat ttcttctgaa tttccttttt caaatgacaa
    28441 ttggccttca gatatcactt tcagcctaaa tggtgtggag ctaggaacat ggacgagccc
    28501 tggagatttt gctgatatcc gcagtaaata tacgcctgat tggtatccag ataatcttaa
    28561 tcaatacggc ttgctgaaga cgatccgaat taccaaacac ttgacgaata tgaatggaga
    28621 acccttgtca aacatcacga tcaacgacat ccctaaagag caagacactt ggcatttgcg
    28681 aatcgaagtg aaagacgatg ccaaacatgt ggggggctgt actttatttg gtaaaggatt
    28741 cggtaattac gatcaggata tcaaattaaa agtttactac agctgatatt tttcttttct
    28801 attttataaa acaaaaacag ccaatcccta tgaagggacg gctgtttttg ctttattgtt
    28861 atttccagaa atcatccaaa acggaaattg gcaaatgtcg tttatgttgt gtcttgttcc
    28921 accatgcttc gattttttct tgtgcttgag cgggaacttc ttttccttct agataatcat
    28981 caatttcatt atatgtgaca cctagtgcga cttcatctgc aatcattggt ttcccatctt
    29041 caagatctgc agtcggaatt tttgtgtata atttttccgg tgcattcaat gcttgaagca
    29101 attgtttccc ttgtcgtttg tccaaacgga ataatggcaa aatgtcggct ccaccatcac
    29161 caaatttcgt gaagaatcca gtaatgtttt ctgcagcatg atctgtccct aatacggctc
    29221 ctgctctttc tccagctacg gcatattgcg taatcatacg ttgacgtgct ttgatatttc
    29281 ctttgttaaa atcgctgatt cccacacctg cttcgcttaa tacttgtact tgtgcatcaa
    29341 cagctggttt gatatttact cgcagactaa catcgggctg gataaagtca agagcagctt
    29401 ttgcatcttc ttcatctgct tgttcaccat aaggaagacg aacagcgata aattgatagt
    29461 tttcatcttt tgtttcttca cgcatttctt ccatcgttag ttgcgccaaa cggcctgcta
    29521 aagtagaatc ttggccgccg ctgataccta gtacaaaagt tttaagaaac ggatattttt
    29581 tcaaatatgc tttcataaag tcaatacttt tacgtatttc ttcttttgga tcaattgttg
    29641 gtttgacacc taattcattg ataatttctg tctgcaggct catttattct cctcctatgc
    29701 ttctgtatga agttctgcta cgcttttttt cattcgttcc atgatagcca ttttatggtt
    29761 ccagcattca gtagacagat cgactgggta tttttgcgga ttcaagtctc gtttatactc
    29821 ttcccatagt gaatctagat tttcttttgt atattctttg atttcgttta gtgcaggtaa
    29881 ttcgtaaact ctttttcctt cgataaagat atcttgtaaa actgggcgag ctgtaaagtc
    29941 tcgaacagtt ttattgataa acgtatgcac tggatggaac atgaagatag cttcttcttc
    30001 tcgagggtct tcatcccata atgtcacgta gtctccttca gatttcccat caaaatttcg
    30061 agtgatgcgc cacacttgtt ttttccctgg agtagttact ttttctgcat tgcttgataa
    30121 cttgatcgta tccatcattt ttccttcatc atcttcgatc gatactagct tgaatactgc
    30181 accaagggct ggctgatcat aggctgtgat caattttgtc ccaacacccc atacatcaat
    30241 tttagctttt tgcatcttta aattcaaaat cgttgcttca tccaagtcat tcgaagcata
    30301 gatttttgct tccgtaaatc cggctgcatc taattgttcc cgaacacgct tagatatata
    30361 ggccatatct ccactgtcga tgcgtacgcc ttggaagttg attttgtcac ctaattcttt
    30421 cgccacgcga atcgcacttg gcacacctga tttcaacgta tcataagtgt cgactaagaa
    30481 cacgcagtct ttatgggttt tagcgtaggc cataaatgct tcataatcat ttccataaga
    30541 ttggactaac gaatgagcat gggtcccgct agctggtatc ccaaatatct tcccagcacg
    30601 tacattgctt gttgcatctg ctccgccgat atatgccgct ctagttcccc agactgctgc
    30661 atccagttct tgtgcccgtc tagttccaaa ttccaatagt ggatcttctc cgatgacaga
    30721 tttgatacga gctgcttttg tcgcgatcaa cgtttggaaa ttgaccatat tcaacagtgc
    30781 tgtttctact aattggcagt gcgctaaagg cccttccact tggattaatg gttcatttgc
    30841 aaaaactagt tctccttcac gagcagaacg aactgttgct ttaaattcaa aattttgtaa
    30901 atactccaaa aaaccttcag gatagacttc taatccacgc aagtaagcaa tatcggaatc
    30961 attgaacgtc agattctcta aataattgac taaccgttcc aatcctgcaa aaaccgcgta
    31021 tccgtggtta aaaggcattt ctcgaaagta acattcaaaa acagcatgta aatcagctcg
    31081 acctaattcc cagtatgtct gcatcatatt gatttgatat agatccgtat gcagcgtcaa
    31141 actgtcgtct gggtaagtat tattcatctt gtattactcc tcttggcaag ttttcatttc
    31201 ctacccatta taacaaaaaa aattaatagg aaaagaaatc ttccattttt tctgaaaaag
    31261 cacatgtttt acggatattt cttgctaaaa atgaactcca tctgctaaaa ctatcctttc
    31321 ttattggtat ttaacctaaa cgagaaagaa tacttatttt attcaatgta tccgagacac
    31381 gaagaaatta gcgttttcta tttttcttac ttgataaaat aagtagattt tcctactaca
    31441 atcgtagccg ttaagatacc gatgattgaa caaatataac caaatgtctc tacaacataa
    31501 tcaatggcaa aagtttttga agcaataata cgtctttcct tttttgcttc ttatccccaa
    31561 agaaacggat ataacttatt atgtattgaa tttttttgga aaggcgaatt atgacaagat
    31621 ataccttgcg ttgaaaaaac tcgcggaaaa aatatcacac gtttttaaaa agatacaaag
    31681 taagaaagca gctgtcaata gaatggaaac aaaaatggcg agacatcaat cagtctcgcc
    31741 ttgtttttta ttgtatacat tagatatgga gcaggttacc ataaagatga ttattagtcg
    31801 ttattaaata gaatccttcg tatttttttg ttctatcact acaatatctt tatttggacg
    31861 tttgaattgt attccggcac gttctagctc ctgtttcact cctacatgga ctttgacatc
    31921 ttctcgtgtc atttttaaaa cgtgatgaat cgcatagtcg atttcttgcc ttgtgatttt
    31981 tttcttcgta ttgatcatca cgacttgctt tttatcatat gtgtctacca atagcagttg
    32041 tgttcgatct aacgaaaata atttgtaata attaatagcc gtattcccaa aaaagaattc
    32101 cactacttta ggataaaatg tcttaatatt taaattacta ttcacaggtg tttcgattag
    32161 cttcatctca ttcctctttt ctatatagtc gatttagatc caatcttaag ttatgttacc
    32221 atctttccta gcattaagga atcggttaca attgaacctt taaagacaaa aggatttacc
    32281 ttttatcact taatagtata acataatttc gtgagaaaaa aatacaattt tttcttgacc
    32341 atctcgttac gtaaaggatt atgcttacat ttgtcaggag gaaaaataat ggagtacaca
    32401 ataaagaaaa tgtcagagat atcaggagtc agtccgagaa ccttgcgctt ttatgatgag
    32461 attggactgc taaaaccagc taggatcaat tcttcaggtt atcggattta tggcaaaaaa
    32521 gaagtcgatc gtctgcaaca catcttattt tatcgaacaa tggcattcaa gttggatgat
    32581 atccaagagg tcttagacaa tccatctttt gaccaccaga aagctttaat caagcatcag
    32641 caaatgttgc tggaaaaacg ggcacaaatc gataccctct tgacaactgt ccaacagaca
    32701 cttgatatgt acgaaggagg aagaaaaatg agtgacaaag aaaaatttga agggtttaaa
    32761 caacaaaagt tgaaagaaaa tgaagaatcc tatggacaag agattcgaca aaaatacgga
    32821 aaaaaaaagg ttacagcatc caatgaaaaa tggatgaata tggataaaga aacgtatgaa
    32881 gaaatgcaaa ctgttgaaaa gcagatgtta tttgatctca ctctttactt aaaagaacca
    32941 gaagatgaaa gcttagcaga ccgtatcttc caagctcata agaagtggtt ctcttacagc
    33001 tggcctgaat atcagccaaa agcgcataaa ggactaggaa tgatgtacga agccgatgaa
    33061 cggttcactg catattatga cgagcgaagt ggtcatggtg cagcaaaagc tttaaatgaa
    33121 attatccaaa aaaaggcgta atcttttaca tgatccaaca aaaaagtaca cgtaaaatga
    33181 cctaataaca aaacagcaga cacccaagtt agatttttat gctaactttt ggtgtctgct
    33241 ccattctttc acaagttttt tcatcctact ttaaatatat tcatcgttga ataaacgtat
    33301 aaggtatttt cactttatcc tttttttctg taagaaacaa ttgaaatgcc atttctccgc
    33361 aagcttccag atggtggtcg atggttggga aaccaagaag ctcgctgatc agcaaattat
    33421 cgcgtccaat caccactggt gtcttatctt gcagatagct ttgcaagatg cctgctgcaa
    33481 ccatatccga atttgtaaag acaccatcaa ctccaagctc tttaaaaaag aagcctgctt
    33541 gttttccttt ctctgcatct atacaatccc aaaaaacaag tgattcatca aaagatggaa
    33601 aaagttgatg acatagctgc aaagtgattt ttgagttata acttagcctg ccacttcgcc
    33661 ccaacgtgat gcccaattgc tttatacctt gctgcttcaa ataagtaaga ccttctgtaa
    33721 ttgattgttc tcgatcaata aagacacagc ttgcctgttt tcccttgatt tcttcacaaa
    33781 aaactagagg tccatatttt tgatatgcca ataaatcatc tagtgtattg gcacgagacg
    33841 taatgatcaa tccatcgaaa gctttagctg caaattcttc aagatacctt cgctcttttt
    33901 ctttatgata gcccgtcggt aaaagtgtca ctttgtaatc ataatcaaac gctgcttcaa
    33961 tgatcccact gatcaactga tcaaaatacg gatgattggt ataaggcaga atcactccaa
    34021 tgcttttcgt ttttccataa cttaactgtc tggcatttgc attagggata taatcgactt
    34081 ctttcatgac cgcaagtatt tctttccttt tttcctcatc tacatatgga tgtccattga
    34141 ttacacgtga aatagtagaa actgaataac ctgtgagctt tgcgatatct cgaatgtttg
    34201 ccactgctgc ttcgccacct atacaaaaag attttcttgt aaatagtcta ccacagctgg
    34261 accgcattca aaaaactgat acatttttcc ttcatgcgaa aaactgatcc gctgcccgcc
    34321 ccaatatata tcttttgtca aattttcgat ttcattcatt ggtatttctt ttactatatt
    34381 cctattcaac ccttgaaaac tgtacaacgt taatacggat cccttgaact cgacaaacgc
    34441 ataaaaataa ttgttcgttg tgccatctgt tatctctaca taagtattca ttgcaagccc
    34501 tccttttgat actttgagta tagtgtatga aacgcgtctc atagcaagga gtttgttttg
    34561 ccaaagaaaa gttttattcc tctttcatta ttaccaaagt ctcttatttt aaattgaata
    34621 cattataatg atactgtaaa tatatcttta ttattagtga at
//
LOCUS       DASHHB010000028        33817 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.028, whole genome shotgun sequence.
ACCESSION   DASHHB010000028 DASHHB010000000
VERSION     DASHHB010000028.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33817)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33817)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33817
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.028"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            299..1960
                     /locus_tag="U2K71_001496"
     CDS             299..1960
                     /locus_tag="U2K71_001496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM7315581.1"
                     /translation="MKKKFTFGLVAVCGLVLAGCYGGSSATSSSSAKSGSADGGGVFN
                     LVVPQEMPTADLSVATDTISFTALNNVYEGIYRLDEDSKPQPAGASELAEVSEDGLTY
                     KIKLREDAKWSNGDPVTAADYVYGWQRTVDPATASEYAYLYAPVENAEDITAGKKDKS
                     ELGIKAVGDYELEIKLTKQTPYFQYLLAFPSFFPQKQSVVEENGDAYASASDKAVYNG
                     PFTLEDFDGPGTDTEWTYKKNDHYWDKDTVKLSEIKVSVVKESSTALNLFKDGQADDV
                     ILSGELAQQNANDPAYTSVKEARTSYIEFNQREKNSPFNNVNLRKAISYSIDREALVK
                     QVLGDGSVVSTGLIPSDMVKSPTTNEDFAKEAGKLVSYDKDKAKEYWEKAKKELGVDS
                     LEFDLMASDDDSSKKVIEYVQNSIQENLDGVTVKPTPVPFSVRLDRSTSGDFDAVLGG
                     WAADYADPSSFTDLFVTGNSYNRGRWSNAEYDEAVKAASSTDAGDEQARWDDFQKAIK
                     IISDDMGVVPVYQKAEGHLVNEKIKGIVHHAAGASWDYKWTYVEE"
     gene            complement(2030..2377)
                     /locus_tag="U2K71_001497"
     CDS             complement(2030..2377)
                     /locus_tag="U2K71_001497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3899 domain-containing protein"
                     /protein_id="HEM7315582.1"
                     /translation="MKKKSIPYAVAFLLILVILIKNLINHSFTLIQLSNDLFLWSLPF
                     LIIGGFLWVFSSGFFDHFQRSVHLARTRNRKKKPEFSTLSSASYGMYSFWLIIAGILI
                     ALSAIFMLFSLLG"
     gene            2461..3402
                     /locus_tag="U2K71_001498"
     CDS             2461..3402
                     /locus_tag="U2K71_001498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016184893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315583.1"
                     /translation="MNSYIKYVLKRVFFMIITLWLIATITFFLMQLLPGTPYTNQERL
                     SPETIEMLNKQVGLDKPVIVQYGIYLSNLLQGDFGISFQFKNQPVAHLLAGRIGPSLQ
                     LGLQAIIFGTVLGTILGTISAMKQNTWADTSSTLVAILGRSIPNFVFAVLLQYIFAIK
                     LQVLPIAKWDGFVYTILPTIALAMSPLADSARFIRTEMVEVLHSDYVELAKAKGLSRW
                     EIAFKHGLRNSLIPLMTLLGPLAVALMTGSLVVENIFAIPGIGEQFVKSITTNDYPTI
                     MAVTILYSFMLIFVILIVDLLYGLVDPRIRVSEGSRG"
     gene            3403..4446
                     /locus_tag="U2K71_001499"
     CDS             3403..4446
                     /locus_tag="U2K71_001499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315584.1"
                     /translation="MEMIPKRYETVADIPADEFLPLQKNTEEEREQITAPSLNFIQDS
                     WRRLKKNKAAVISMVLLLVIILISVITIFVSPHDPTAQNVAYINLPPRIPGINIDGLN
                     GKAMVGGELVDKYAQANVPSNVNFFLGTDGLGRDVLSRLFMGMRISLLIAFIAALLDV
                     TIGVAYGLVSGLLGGRVDNVMQRFLEILSGIPNLVVMILMLVVFEPGIFSIVAAMAIT
                     NWIPMARIVRAQTMKLKDQEYVLAGLTLGESRMKIAFKHILPNISSVIIIQMMFSIPS
                     AIFFEAFLSFIGLGLTPPSASLGTMLSEGYKTFLYLPYLLWIPAVTLSVIMIGFNLLA
                     DGLRDAFDPKMKE"
     gene            4452..5522
                     /locus_tag="U2K71_001500"
     CDS             4452..5522
                     /locus_tag="U2K71_001500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315585.1"
                     /translation="MTKILEVKDLEISFDTYAGKVRAIRGVNFHLNKGETLAIVGESG
                     SGKSVTTRSIMRLLSSNANIDAGQILFKGNDIVGKSEREMQKIRGKEIAMIFQDPMTS
                     LDPTMPIGKQVAESLRKHNKVSKKEGQKAALELLKLVGIPEAEKRINSYPHQFSGGQR
                     QRIVIAIALVCYPEILIADEPTTALDVTIQAQILELLKEIQTKIDTSIIFITHDLGVV
                     ANVADRVAVMYGGRIVEVGTSEEIFYNPQHPYTWGLLGSMPTLDSANDRLYAIPGSPP
                     DLLNPPKGDAFYPRNEFAMKIDAEQEPPFFELSKTHKAATWLLAPQAPKVTPPEEIQR
                     RWAIFKEKQKHTYGQGGIADAK"
     gene            5512..6465
                     /locus_tag="U2K71_001501"
     CDS             5512..6465
                     /locus_tag="U2K71_001501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7315586.1"
                     /translation="MPNKLLEVKGLKQYFNVGKKNEVHAVNDISFHIYEGETFGLVGE
                     SGSGKSTTGRTIIRLNEPTDGEILFDGQDITKIKDKKGLTKFRHDVQMIFQDPYASLN
                     PRMKVRDIIAEGIDVNGLAKSSEERAKKVDDLLRTVGLNPSHGTRYPHEFSGGQRQRI
                     GIARALAVKPRFIICDEPISALDVSIQAQVVNLLQDLQKEHQLTYLFIAHDLSMVKHI
                     SDRIGVMHNGLLLEMGTSDEIYNHGVHPYTESLLSAIPLPDPDHERKRRRIKYQPEPD
                     DGQVRQLREIAPEHFVYATEQEVPYYAKKLKRQKESLLVAN"
     gene            6477..7487
                     /locus_tag="U2K71_001502"
     CDS             6477..7487
                     /locus_tag="U2K71_001502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM7315587.1"
                     /translation="MLKAFKFRMYPTEEQKQQLIRTFGCARFTYNHLLKKRQKSWQQT
                     GVANFSLTPATLKKEYPFLKEVDSLALANAQLNLDRAFRNYFKGRASFPKLKTKKSMW
                     QSYTTNNQTRTIYLENGYLKLPKQKELIKINQHRPVEGSIRSATISARYNEEFYVALL
                     CDVSPVKKESLAKWIGIAYHPKTLIQTSRPLEVTLPKFHQTEEKLQHAQRKLNVKVRS
                     AHHRKIRLDQASNYQKQKRKVMDLYLKQKNQREDYLEQLSGKLVKQYDYLFVESFPKE
                     EAHADFSIHDWHKLITKLRYKSQWYNKKFLLINTDGAEESNSVRKSQVVEKMGRHSVI
                     KG"
     gene            complement(7772..8593)
                     /locus_tag="U2K71_001503"
     CDS             complement(7772..8593)
                     /locus_tag="U2K71_001503"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HEM7315588.1"
                     /translation="MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDI
                     VLMCYNKEEDQLKVLLIQRKGHPYRNSWALPGGFVQKDESTGESVLRETKEETGVVIS
                     KENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEPLIAGDDAKEVRWFTLERHSNKIHL
                     SSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTIT
                     EARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE"
     gene            complement(8690..8968)
                     /locus_tag="U2K71_001504"
     CDS             complement(8690..8968)
                     /locus_tag="U2K71_001504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315589.1"
                     /translation="MQKKHLFFTLSIAFLSLAHLIFSYFYIRMYGYFNLHGHLNSFMT
                     AAWILRFIIDVYIVICGFFAIREERYKVLPFYLLFFLFNLILPFIFHI"
     gene            9236..11245
                     /gene="metG"
                     /locus_tag="U2K71_001505"
     CDS             9236..11245
                     /gene="metG"
                     /locus_tag="U2K71_001505"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HEM7315590.1"
                     /translation="MAEKETFYITTPIYYPSGKLHIGNSYTTIACDAIARYKRLMGFD
                     VFYLTGVDEHGQKIETKAEELGVKPQEYVDKMAADVKKLWKTLDISYDKFIRTTDDYH
                     KAAVQKIFDRLLEQGDIYLGEYEGWYSVSDEEFFTETQLAEVYKDDEGNVIGGKAPSG
                     HEVELVKEESYFFRMSKYADRLLEYYEEHPEFIQPESRKNEMINNFIKPGLEDLAVSR
                     TTFTWGVPVKSDPKHVVYVWIDALSNYITALGYGSEDESLFEKYWPADVQMVGKEIVR
                     FHTIYWPIMLMALDLPLPKKVFGHGWLLMKDGKMSKSKGNVVYPEMLVERYGLDALRY
                     YLLRAVPFGSDGVFTPEDFVSRVNYDLANDLGNLLNRTIAMINKYCDGLVPDYASLVT
                     PFDSELSTTAANVVGRYHDAMEKMEFNTALAEIWVLISRANKYIDETEPWVLAKDEEK
                     KAELNSVMIHLAESLRIAAILLQPIMTETPEKIFNQLGLDSETMNLEGLHFGEFPSGT
                     KVVAKGTPIFPRLDMEEEVAFIQEKMSEGTQTNEDTVKWDPEETELVSTKEKQIKFDV
                     FEKVELKVAEVINCQKVEGADKLLQFRLDAGDSQDRQILSGIAEFYPDPSELIGKKVV
                     IVANLKPRKMRGQISQGMILSAEAPDGSLQVIEAPKSMPNGSEIA"
     gene            complement(11724..12311)
                     /locus_tag="U2K71_001506"
     CDS             complement(11724..12311)
                     /locus_tag="U2K71_001506"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315591.1"
                     /translation="MDFKRSYPKLADYLSNRVDFSRKEQQQSHWHWFTLILFGLMTVA
                     TLSQKLILLTLFIAIVALIKGPVMILWGMLYSFLVGLFPPIGILLSAIFFLLNIGTFT
                     KNWRMTLVGIYFYFYPFGVMALSEVMHWDNHWFIAGSLLLGLILLHVMLTKLYQHYGI
                     GRTIFWYVFSIPFALLTALLPSRLKTKIKGYHKIK"
     gene            12486..13808
                     /locus_tag="U2K71_001507"
     CDS             12486..13808
                     /locus_tag="U2K71_001507"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7315592.1"
                     /translation="MKGKLKREINLFGALATVMGTVIGAGVFFKTAAVTASTQSTSLT
                     LLAWLLGGFLTICAGLTVAELATAIPETGGAVKYIEAAYGKLPSFLLGWAQSLIYFPA
                     NIAALSIIFATQLTNLLQLSTDYLLLIAVITAVSVTGLNLLGTKVGASVQSVTLIVKL
                     IPIAVIVIWGLLTPGQGTVQLFPIEAGKDVTFVEGLSSALLATLFAYDGWLGVGAMAG
                     EMKRPEKDLPKAIILGLSFVTVVYLLINFVFLKTLPIDHLAGNLNAASEASDVIFGGI
                     GGKLVTIGILISVYGALNGYTLTGIRVPYAMALEDELPFSKQLTKLSKKFTVPYVPAV
                     FQLAVACIMMSLGSFDFLTDMLIFVMWLFSLLIFIGVFILRKKAPELPRPYKVPLYPI
                     VPIIAILGAIFILGMTMMTQTKLALIGIGVTLIGIPVYYQKKKKRSEE"
     gene            13963..14739
                     /locus_tag="U2K71_001508"
     CDS             13963..14739
                     /locus_tag="U2K71_001508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TatD family hydrolase"
                     /protein_id="HEM7315593.1"
                     /translation="MIFDSHTHLNAEQFNEDIPETIQRAQEMGVTKMAVVGFDTPTIE
                     KSLQLSHDYPNIYSIIGWHPTEAGSYTKEIENKLQEQLTLPKVVALGEIGLDYHWMED
                     PKEVQDKVFRRQIAIAKEMNLPISIHTREALEDTYQILKEEDVRDIGGIMHSFSGDYE
                     WAKRFLDLGMHISFSGVVTFKKALDVQEAAANVPIERLLVETDAPYLAPVPFRGKRNE
                     PGYTRYTVEKIAELRNLPTEEVAAQTWTNAHRLFGLSEHG"
     gene            14732..15301
                     /gene="rnmV"
                     /locus_tag="U2K71_001509"
     CDS             14732..15301
                     /gene="rnmV"
                     /locus_tag="U2K71_001509"
                     /EC_number="3.1.26.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease M5"
                     /protein_id="HEM7315594.1"
                     /translation="MDKMKIQEIIVVEGKDDTRRLREVVDADTIETIGSAINEEILMQ
                     IEHAQETRGVIVFTDPDFSGEKIRKIIMEAVPQAKHAFLPRTQAKPKKKGSLGVEHAS
                     DAAILEALKKVVTPDISQTAVSEITRQDLLDYGLIAGAQAKEKREKLGDELRIGYTNG
                     KQLEKRLKMFRITPKELAQAMKIVEEQHE"
     gene            15294..16178
                     /gene="rsmA"
                     /locus_tag="U2K71_001510"
     CDS             15294..16178
                     /gene="rsmA"
                     /locus_tag="U2K71_001510"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HEM7315595.1"
                     /translation="MSEYREIATPSRTKEILLKHGFSFKKSLGQNFLTEPNILRKIVE
                     TAQIDEHTNVIEVGPGIGALTEQLAKHAKQVVAFEIDDRLIPVLADTMQPYDNVTIVH
                     QDILKTDLSTAVRETFHEELPLKVVANLPYYITTPIMMHFLESDLVVDELVVMMQKEV
                     ADRISAEPGTKAYGSLSIAVQYYMEASLAFIVPKTVFVPQPNVDSAILKLTRRDIPAV
                     EVTDEKAFFRLTKAAFQQRRKTLWNNLQHSYGKDDQTKAWLAKSLETAGIDPKRRGET
                     LSLQEFAALSNAMSENMQ"
     gene            16629..17258
                     /locus_tag="U2K71_001511"
     CDS             16629..17258
                     /locus_tag="U2K71_001511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315596.1"
                     /translation="MRKNSVRKLTISALLIGMGVIIPMVMPKIMIPPASFTLASHVPL
                     FIAMFFTPGVAVAVALGTTFGFFLTTPVIIALRALSHLVFALIGAWYLQKHPSIVLKN
                     GQFTFANWRFQGFNFVIGVIHSLAEMLVVSIFYFIGNMPETYYSQGYFYTVFILMGIG
                     GLIHSLVDYNIAYFIAGTLSKYFDIPVFTAAKKENRKESIQGYAKKTTY"
     gene            complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="U2K71_001512"
     CDS             complement(17382..18491)
                     /gene="ugpC"
                     /locus_tag="U2K71_001512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HEM7315597.1"
                     /translation="MVEMALKNVYKKYDNAENYSVTDFNLSIKDREFIVFVGPSGCGK
                     STTLRMIAGLEDISEGELNIGGKVMNDVAPKDRDIAMVFQNYALYPHMTVFDNMAFGL
                     KLRKYDKADIKKRVDNAAEILGLTEYLKRKPAALSGGQRQRVALGRAIVRDAKVFLMD
                     EPLSNLDAKLRVAMRAEIAKLHQRLETTTIYVTHDQTEAMTMADRIVIMKDGFIQQIG
                     TPKEVYDTPVNMFVAGFIGSPAMNFFNVTLKNGVITDGHGLKLRIPEGKNKVLVEKGY
                     EGKEVIFGIRPEDIHSEQVALEASPEAVVKSEVVVSELLGAETMLYTKTGSTEFISKV
                     DARDFHKPGEIVDLAFNISKGHFFDKETEQVIKLP"
     gene            18700..19122
                     /gene="mgsA"
                     /locus_tag="U2K71_001513"
     CDS             18700..19122
                     /gene="mgsA"
                     /locus_tag="U2K71_001513"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HEM7315598.1"
                     /translation="MKIALIAHDRKKPMMVKLATAYKTILEKHELFATGTTGQRITEA
                     TGLPIHRFKSGPLGGDQQIGAMISEDQLDLVIFLRDPLAAQPHEPDVTALIRLSDVYE
                     IPLATNIGTAEVLLRGLEAGFLDFRRIVHEIDKHPLSF"
     gene            19445..19981
                     /locus_tag="U2K71_001514"
     CDS             19445..19981
                     /locus_tag="U2K71_001514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate family ECF transporter S component"
                     /protein_id="HEM7315599.1"
                     /translation="MKKHRLDARMIAIMGLLIALMVTLSRLVAIETPFIKISVTFIPQ
                     VIMGILFGPFWSGIGAVLADLVGMALFSKSAFFIGFTLNAFIEGAIYGFFFYRKEITW
                     KNAILATLSVTLIINLFLTPLWLALMYHVPLFSWVVWAPRLLKTVIWLPIQSIAIYYV
                     GRSIPYKKILRSLAIHAK"
     gene            complement(20021..21784)
                     /locus_tag="U2K71_001515"
     CDS             complement(20021..21784)
                     /locus_tag="U2K71_001515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315600.1"
                     /translation="MKVKKLSLQTLRRFIPYLTAYAKDIWLAVILGIINGIATVVMTL
                     QIGQSIDQMIGEGQVRFNRLFHLLILFGGIVLINVVSQWLIQVLSNRLAYLSVAKLRK
                     DAFAHLNRLPLRYYDQNSHGNIVSRFTNDMDNISIAISAAFNQLFSGVAIIILSFIFM
                     MRLSPLLTLVVLCSTPIIFLVSWIVARASQTNFDRQQRIIGDISGFVTERIGNQKIVK
                     AFQQEEASQQQFELLNADLYEKGQRAQFSSSLTNPSSRFVDHLAYLSIGLVGGLLALK
                     SGSSITVGVISSFTIYSSQFSKPFIELSGITTQLQTAFSGLERAFDLIDQPEEKPDLP
                     DAYALEPKNIKGKVSFDHVSFSYDPKQRLIEDFNFTAYPGQTIAIVGKTGAGKSTLVN
                     LLMRFYDTTKGTITVDDHDIRSIQRDTLRKSFGMVLQDTWLFDSTLRENLRFGNPEAS
                     DAEINDALKASYMYEFVERLPEKLDTKIGGQGLKISDGQRQLLTIARTMISNPPMLIL
                     DEATSSVDTLTEKKIQQAFLAMMEGKTSFVIAHRLSTIKNADQILVMDQGAIVEMGTH
                     DELLQKDGYYRRLYQAQFTAQ"
     gene            complement(21787..23502)
                     /locus_tag="U2K71_001516"
     CDS             complement(21787..23502)
                     /locus_tag="U2K71_001516"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315601.1"
                     /translation="MIQLLRYAKDYRKQIILGPFFKFLEAVFELVLPLMMASLIDNGL
                     KMNDRGKIIEMGLWMVAMSVIGLICAIICQYYASVASQGFGTELRNQLIKKINTFSHN
                     ELNHFGTDTLITRMTNDINQLQLALAMVIRLLIRAPFLSIGSVVMAFVIDWEVGLFFL
                     ALLPIFSIILFFIIKKTVPLYQKVQEKLDQLNEHVSQNLSGVRVIRAFAQTKKETEKF
                     DKSTNDLANNYLRVSNISALLSPATTLIMNVGIICLLTVGGIKVNIGSLQQGQVLALI
                     NYMNQMLLALIVVSNLVVIFTRAEASGNRVKEVLDTENSILEAEVSTTPDFSSEAIIA
                     FDHVDFRYTPDSGLSLQNITFQLKRNTVLGIIGPTGSGKTTLTQLIPRFYDVSSGTVI
                     MDGMDVRSWQLDHLRQQIALVPQTSVLFTGTIRENLQWGKKDATEKECWEALKIAQAE
                     EFVRQLPNGLDTKVMENGKNFSGGQKQRLTIARALIAKPALLILDDSLSALDYQTDLD
                     LRKALQTYLGSTVIIISQRVRSIQEAHHILVMDQGKIAAQGTHEELLAQSAEYQEIVA
                     SQEEE"
     gene            complement(24281..24505)
                     /locus_tag="U2K71_001517"
     CDS             complement(24281..24505)
                     /locus_tag="U2K71_001517"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315602.1"
                     /translation="MKSDKYKNRKHTSWKTFREIIGSYLAVVVFLPLLYMAMTEQTRF
                     IGVDTQYIAETGFLFIFILIFAFYKSFHTK"
     gene            24696..27473
                     /locus_tag="U2K71_001518"
     CDS             24696..27473
                     /locus_tag="U2K71_001518"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM7315603.1"
                     /translation="MNKLLKKEILCYLENQTAFFDLEHMNEVFTAQRLADHFGVKRNT
                     VSHYLNQLNDEQLLVKINSRPVIYFHKEAFEKQFFKLRTNFYFSIDDLKEEQPFFAQP
                     KDLFSFMIGYDASLKESIEQIKTALYYPDGGLPLLITGESGTGKSYLVNLVYQYCLLH
                     DLLEDSAPFITFNCAQYADNPELLTSNLFGHVKGAYTGAEENKKGAFEAADGGILFLD
                     EVHRLTPEGQEKLFTYLDQGVIYRMGEPNLSRRIKTRLFFATTEEITSHFLTTFIRRI
                     PIRIELPALNQRSRNERLELIYSFFLEEQRKMGSPLSVSGPALSLLTSQKLSGNIGEL
                     KNIVKVSAAKAYAEQREQPEIHVTIHHLQKELLAQPITKNTAQQGIYITQDQTLEQLM
                     EKQQPEQQRIVKSFEQILIDFKKNNCLLYSCEGKLKQEVIQLFDFLLFETSRQEKHEL
                     LVYWTQSIRETFRQMETAYQIKFDGNSVYALSYYLYQRSTIKWLPEEHDIIQLIKELE
                     QQVSQSYPSSYHYVQRILELSKIKLDIEDTSMDRILLTIYLKKAKWSKEKRLPKAMIA
                     AHGYATASSMANVVNRLLQEDLFESFDMPLDVTPQQIASEILDYCENSDVSNGLIILV
                     DMGSLKEIHQFFKKQLSVPLLILNNVTTPLAITVGECLQKNVSLEEIAEETVRQIQPE
                     WRLLYPEENKPKALITTCFTGIGTAVHLSELLEKSLPTACQLKIIPYEYQQLKDKKNS
                     DPLFSIYEVVGMIGTTDPDITNIDYLSLEDLISGEKMSVLAEWLDTTMNASEKEIFNQ
                     RIIRNFSLEKVLDSVTILDTEKVMGEIDHFMRELEVQLNRSISNPKKLALYVHVSCLI
                     ERLIRQMPIETYQGLEKWKQCQKEGLSAIKSAFSVIEEHYSVMIPNSEIAYIYDILSE
                     DTEFLSIEEEF"
     gene            27554..27973
                     /locus_tag="U2K71_001519"
     CDS             27554..27973
                     /locus_tag="U2K71_001519"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS mannose transporter subunit IIA"
                     /protein_id="HEM7315604.1"
                     /translation="MDREIILASHGKFASGILDSLELIYGKNHSITVFDCYTDENFDL
                     AETVRQLFMRYKDKEIIVLTDLFGGSVNNEFLQYINRDHVYLVAGLNLPLLIELVSRL
                     DAEVETTVLIQQVLTDSKKMIQFCNERLNQKIEEDEF"
     gene            27985..28455
                     /locus_tag="U2K71_001520"
     CDS             27985..28455
                     /locus_tag="U2K71_001520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7315605.1"
                     /translation="MIVLTRVDHRLLHGQVAFSWTQTIGADCILIANDDVPTNEIRKT
                     TIKLAKPQGVKLVIKSIDDSIAALKSGVTDKYKLFIVVESIEDAYKLAEAYPEIKTIN
                     LGGIKAKPGTRSIGKAVNILPEEEERLKRLIDKQIEVEIRQVPADKKVDVREVL"
     gene            28472..29251
                     /locus_tag="U2K71_001521"
     CDS             28472..29251
                     /locus_tag="U2K71_001521"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7315606.1"
                     /translation="MIVQAVLLGIVAFVAQSEYALGTSLLSRPIVTGLFTGIVLGDVK
                     TGIIMGATLELAFIGSFSVGASIPPDVVTGGILGTAFAITAGAGTETALLLGLPIATL
                     TLILKNIYLGLLIPIMNHKADNYAEEGNYKGVEHMHLLAGIGLSLMLGVVVMVSYMVG
                     SNTIGNLLNMIPDFVQKGLSVATGLIPALGFAMLARLLINKQVAPYFFLGFAIASYLE
                     IPVTGVAIFGAILAVVVVNIMNVRQLPLQTTAGEVEEDEEF"
     gene            29238..30059
                     /locus_tag="U2K71_001522"
     CDS             29238..30059
                     /locus_tag="U2K71_001522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM7315607.1"
                     /translation="MKNSEIKNSKITKKELNHVFWRSFQMEFSWNYERQMNMAYVYAM
                     IPILKKLYQSKEEMAAALKRHLEFFNTTPHIVTLILGITAAMEEENKDDPEFDVTAID
                     SIKTSLMGPLAGLGDSFFWGTLRLIATGVGTSLAMQGNILGPILFILIFNIPHILFRY
                     LATGWGYRLGTGFLKKIQANGMMESLTLGASIIGLMVVGAMTATMITINIPLKIGSGE
                     NAVTVQSIFDDIVPNILCLGAFGIVFYLLKKEVKPLTILIGLAFFGIFGSWIGIF"
     gene            30076..31125
                     /locus_tag="U2K71_001523"
     CDS             30076..31125
                     /locus_tag="U2K71_001523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM7315608.1"
                     /translation="MFTMQDYIYEEKEVLSTILKKNDFSTREHMKKTVNLLILATGSS
                     YNACLAAKPALESYGDLTVDIQEPFYFFNYGKLSPSIDTVIAVSQSGKSASTVEAVKM
                     IQKQGLPVVAITNDVQSPLALEAAQIIDLGAGVESVGFVTKGYSATVLQLLLLGIGIG
                     ISKNKISKKIEQDYMQQLRKIINHLPAIIQKTEEFFDGYQSLFRLAQRFVTIGYGPNW
                     GTAKEAETKLTETIRVPSQGFELEAYMHGPYLEADASHLLFFIEGESVNKERSQKLQR
                     YMSRYVGETLTITTKKARNEKTLGLAIECDEYLSVLALVVPFQLFAYKTAVAKGIDLN
                     KKIFEDFDTVLKSKL"
     gene            31138..32142
                     /locus_tag="U2K71_001524"
     CDS             31138..32142
                     /locus_tag="U2K71_001524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM7315609.1"
                     /translation="MLKFNEEEQIKAIKGALALRPQVEEIIDKLYAEKFDAVYYLGIG
                     GTYASSMQAVTYMNGKSNLPVFVQHAAEYYTTGNKRLTKDSIVILSSVTGTTQEVVKA
                     VEQIKKVGATLIGFIDKANSKLSQLCDFVVTYPAPGTEQIKFFMAADRLMYLNGEFEA
                     YSEYYEQLERYLPTGLVEAEKKADAFGLSFAEKHRRDSMHYFIGAGNQWGAVYSYAMC
                     YWEEQSWLPSKSIHAAEFLHGTLEIVEETTPVTLFLGEDEQRVLSERVAKLLPKICSN
                     YTLIDTKDYPVEGISEKYRGRVLSFLLMHVVTQRIDAHVEQLNCHPLEIRRYYRQFDY
                     "
     gene            32775..33794
                     /locus_tag="U2K71_001525"
     CDS             32775..33794
                     /locus_tag="U2K71_001525"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEM7315610.1"
                     /translation="MKQKIAVLGPGSWGTALAQTLAENGHDVRIWGNVPEQIDEINKY
                     HTNQHFLPDLTIPESIIGYKELAEAVDNADAILFVVPTKAIRSVAQELIGKMNTKPII
                     IHASKGLEQDTHKRISEVLAEEIPEEKRQAIVVLSGPSHAEEVAVHDITTITAASIDQ
                     KAAAYVQKLFMNDYFRIYTNNDVIGVETGAALKNIIAIGAGAIHGLGFGDDAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLIVTCTSVHSRNWRAGNLLGQGHKLEEVLE
                     NMGMVVEGVATTKAAVELAQQLGVEMPITQTIYNVLYNGEDIKKAAKEIMLRDGKMEN
                     EFSLR"
BASE COUNT        11041 a         5992 c         6908 g         9876 t
ORIGIN      
        1 tcagataagg gactgtgaca agacttttgt cacagtttct ttttgtttat tcttttttat
       61 ctttgtatgg caataataga caatataacg aaaattattt aatggctttt ggaagaccat
      121 ggaaaacctt gtacaaagct attttttctg aaaaattcct tgaaacctca cgaaaaaatc
      181 attattttat gaaaaagaca aaaaaaagtg ttgatttttt aataattatt cattaaactc
      241 ttaaacaact tcagaaaata ttaattattc tgaaaattcc aataaagggg tacgtaaaat
      301 gaaaaagaaa tttacatttg gtttggttgc ggtatgcggc cttgtacttg ctggatgtta
      361 tggcggcagt tcagccacaa gcagtagttc agcaaaaagc ggttcagcag atggaggagg
      421 cgtatttaac ttagtcgtgc ctcaagaaat gcctacagct gacttatcag ttgcaacaga
      481 tacaatcagt tttacagcat tgaataacgt ttatgaagga atttaccgtc ttgatgaaga
      541 cagcaaacca cagccggcag gagctagcga gttggcagaa gtcagtgaag acggattaac
      601 ttacaaaatc aaattacgag aagacgcaaa atggtcaaat ggtgatcctg ttacagcagc
      661 ggattacgtt tatggctggc aacgaacagt agacccagct acagcttctg aatatgctta
      721 cctgtatgcg ccagttgaaa atgcagaaga cattacagct ggaaagaaag ataaatcaga
      781 actagggatc aaagcagttg gtgactatga attagaaatc aaattgacga aacaaacacc
      841 atatttccaa tatctgcttg ctttcccatc atttttccca caaaaacaat ccgtagtgga
      901 agaaaatggg gatgcctatg catctgcaag tgataaagct gtttataatg gaccattcac
      961 attagaagat tttgatggac caggaacaga tacagaatgg acttataaga aaaatgatca
     1021 ttactgggac aaagatacag taaaactttc tgaaatcaaa gtcagtgtag taaaagaatc
     1081 ttctactgca ttgaacttat tcaaagatgg ccaagcagat gacgtgatct tgtctggtga
     1141 gttagcacag caaaacgcaa acgatccagc ttatacttct gttaaagaag cgcggacaag
     1201 ctatatcgaa ttcaatcaac gtgaaaagaa ttcaccattc aacaatgtga acctaagaaa
     1261 agctatttct tattctatcg atcgtgaagc tttagtaaaa caagtactag gtgacggatc
     1321 agttgtctct acaggattga tcccatcaga tatggtaaaa agtccaacaa cgaacgaaga
     1381 ctttgcaaaa gaagctggaa aattagtttc ttatgacaaa gataaagcaa aagaatattg
     1441 ggaaaaagcg aaaaaagaat tgggcgttga ttctttagaa tttgatttga tggcttctga
     1501 tgatgattca tcgaaaaaag taatcgaata tgttcaaaac tcaatccaag aaaacttgga
     1561 tggcgtaaca gtcaaaccaa cacctgttcc attctctgta cgtcttgacc gctcaacttc
     1621 gggtgatttt gacgcagtac taggcggatg ggctgctgac tatgcagacc caagcagctt
     1681 tactgatttg tttgtcacag gtaattcata caaccgtggt cgctggagca atgcagaata
     1741 tgacgaagca gttaaagcag cttcatcaac agacgctggc gatgaacaag cccgttggga
     1801 tgatttccaa aaagcaatca agatcatctc tgatgatatg ggtgtcgttc cggtgtacca
     1861 aaaagcagaa ggtcacctag tgaatgaaaa aatcaaaggt atcgttcacc atgcggcagg
     1921 cgcttcatgg gattacaaat ggacatacgt tgaagaataa tcgttaatac cgtgagcatg
     1981 agaaaaaacg aggctgcggc aaatgtcgca acctcgttct catattcact tatcctaaca
     2041 aagaaaaaag catgaaaata gcagaaagag caattagtat tccggcaatg atcagccaaa
     2101 agctgtacat accgtagctg gcagacgaga gcgtggaaaa ttctggtttt ttctttcggt
     2161 tccttgttcg tgcaagatgg accgatcgct gaaaatgatc gaagaaaccc gaagaaaaga
     2221 cccataaaaa accgccgata atcagaaacg gcaatgacca taagaataga tcattagata
     2281 attgaatgag tgtaaaggaa tggttgatga gattttttat taaaataacc agaatcagta
     2341 gaaaagctac tgcgtagggg atagattttt ttttcatttg attgctcctt tatttttggt
     2401 tcattatcta ataatgtagc gaaacatctg tcagaagtca aaagctaggg ggaaacatta
     2461 gtgaatagtt atatcaaata tgtattaaaa cgtgtctttt tcatgatcat cacgctttgg
     2521 ttaattgcaa caatcacctt tttcttgatg caattgctgc cgggcacgcc atatacgaat
     2581 caggaacgtt tgagtcctga aacaatcgag atgctaaata aacaagtcgg tttggataaa
     2641 ccagttatcg tacaatatgg gatttatctt tctaatttgc ttcaaggaga ttttggaatt
     2701 tccttccaat ttaaaaacca gccagtcgca catttgttag ctggaagaat cggaccatcc
     2761 ttgcagttag ggttacaggc aattatcttt ggaacagtcc tagggacgat tttaggaacg
     2821 atctctgcca tgaagcaaaa tacatgggca gatacatctt ctacactcgt agcgatatta
     2881 ggtcgttcca ttccaaactt tgtatttgct gttttattac aatatatctt tgcaatcaaa
     2941 ttacaagttt tgccaatcgc aaaatgggat ggctttgtgt acacaatctt accaacaatt
     3001 gcgttggcca tgtcgccgct tgctgactct gctcggttta tccgtacgga aatggtggaa
     3061 gtactgcata gtgattatgt ggaattagca aaagcaaaag gattgagccg ttgggaaatc
     3121 gcctttaaac atggtcttcg aaatagtttg attccattga tgactttatt aggaccgtta
     3181 gctgtagcat tgatgaccgg atcactggtg gtagaaaata tctttgctat tcctggtatc
     3241 ggggaacaat tcgttaagtc aatcacaaca aatgattatc ctacgatcat ggctgtaacg
     3301 atcctttact catttatgct gatttttgtt attttgatcg tggatctatt gtacggtcta
     3361 gttgatccaa gaattcgtgt ttcagaaggg agccgaggat aaatggaaat gataccaaag
     3421 cgttatgaaa cagttgcaga tatccctgcg gatgaatttc ttccattaca aaaaaatacc
     3481 gaagaagaac gtgaacagat tactgcacct tctttgaatt ttatccaaga ttcatggcgc
     3541 cgtttaaaga aaaacaaagc tgccgttatt tcaatggttt tactattggt catcattttg
     3601 atctctgtga tcacaatctt tgtttctcct catgatccaa ctgcccaaaa cgtggcttat
     3661 atcaatttgc ctcctcgtat tccgggaatc aatatcgatg gattgaatgg aaaagcaatg
     3721 gttggtggtg aacttgttga taaatatgct caagcaaacg tcccttcaaa cgtaaatttc
     3781 tttttaggaa cagacggttt gggacgtgat gtattaagcc gcttatttat gggaatgaga
     3841 atctcgttgc tgatcgcatt tattgctgca ttactagatg taacgattgg tgtagcttac
     3901 gggttggttt ctggtttgtt aggcggacgt gtcgataacg tgatgcaacg ttttttggaa
     3961 atcttatcag gaattccaaa cctcgttgtc atgattttga tgttggtcgt ttttgaacca
     4021 ggaatttttt caatcgtggc tgctatggcc atcacaaact ggataccaat ggcacgtatc
     4081 gttcgtgcac aaacaatgaa attaaaagat caagaatatg ttttagctgg attgacatta
     4141 ggtgaatcaa gaatgaaaat tgcattcaaa catattttgc cgaatatctc tagtgtcatt
     4201 attattcaaa tgatgtttag tattccatct gctattttct tcgaagcctt tttaagcttt
     4261 atcggtttag gtcttacgcc tccgtcagct tcattaggaa cgatgttgag cgaagggtac
     4321 aagacattcc tgtatctgcc ttatctatta tggattccgg cagtaacttt atcggttatc
     4381 atgattggct ttaacttact tgctgatgga ttgcgtgatg cctttgatcc taaaatgaaa
     4441 gagtgaagac gatgacgaaa attttagaag tgaaagactt agaaatctct tttgatacgt
     4501 atgctggaaa ggtacgagcg attcgcggtg tcaattttca tttgaataaa ggagaaacat
     4561 tggcgatcgt tggggaatct ggaagtggaa aatcagtgac gaccagaagc atcatgcgct
     4621 tgctgtcaag caacgccaat atagatgctg gtcaaatcct ctttaaaggt aacgatatcg
     4681 ttggaaaatc agaaagagag atgcaaaaaa tccgtggtaa agaaatagcg atgattttcc
     4741 aagatccaat gacctcttta gatccgacga tgcccattgg gaagcaagtc gctgagtccc
     4801 ttagaaagca taataaagtt tcaaaaaaag agggacaaaa agctgcatta gaattgctta
     4861 aattagttgg aatacccgaa gctgagaaac gtatcaacag ttatccgcat cagttctcag
     4921 gaggtcagcg tcaacggatc gtgatcgcga ttgcactggt atgttatccg gaaatcctga
     4981 ttgctgatga acctacaaca gcgcttgatg tgactattca agcacagatt ttggagctgc
     5041 tgaaagaaat tcagacaaaa atcgatacat caattatctt tatcactcat gaccttggtg
     5101 tagtagcgaa tgtcgctgat cgtgtcgctg tcatgtatgg aggaagaatc gttgaagttg
     5161 gaacatcgga agaaattttc tacaacccgc aacatcccta tacttgggga ctgcttggtt
     5221 caatgccgac attagatagt gcgaacgatc gattgtatgc tattcctggt tctccaccgg
     5281 atttattgaa cccgccaaaa ggagacgcat tctatccaag aaatgaattc gcgatgaaaa
     5341 tcgatgcaga acaagaacca ccattttttg aactatcaaa aacacacaag gcagcaactt
     5401 ggctacttgc tccgcaggca cccaaagtga caccgccaga agaaatccaa cgtcgctggg
     5461 ctatctttaa agagaaacaa aaacatactt acggtcaagg aggaattgct gatgccaaat
     5521 aaactacttg aagttaaagg attgaaacag tatttcaatg taggaaaaaa gaatgaagta
     5581 catgctgtaa atgacatcag ctttcacatt tatgaaggag aaacattcgg tttagttgga
     5641 gaatctggaa gcggaaaatc cactactggc cgtacgatca ttcgcttgaa tgaacctact
     5701 gatggtgaga tattattcga cggtcaagat ataacgaaga tcaaagataa aaaaggtttg
     5761 acgaaattcc ggcatgatgt tcagatgatc tttcaagatc catacgcttc gttgaatcct
     5821 cgtatgaaag tcagagatat cattgccgaa ggaattgatg ttaatggact agctaaatca
     5881 tctgaagagc gtgcaaaaaa agtcgatgat ctattgagaa ctgttggatt gaatccaagc
     5941 cacggtactc gttatcctca tgaattttca ggtgggcaac gccagcgtat tggtattgca
     6001 cgagcattgg cagtaaaacc gcgttttatc atttgtgatg aaccaatttc tgcactagat
     6061 gtgtcgatcc aagcccaagt ggtcaatttg ttgcaagatc tccaaaaaga acatcaattg
     6121 acctatctat ttattgcaca tgatttgtcg atggttaaac atattagtga ccgaatcggt
     6181 gtaatgcata acggcttgtt attagagatg ggaacaagtg atgaaatcta taatcacggt
     6241 gttcatcctt atacagaaag tttgttatct gctattcctc taccagatcc agaccatgag
     6301 agaaaacgca gaagaatcaa atatcaacct gaaccagatg acggacaagt acgtcaactt
     6361 cgtgaaatcg caccagaaca ttttgtctat gcaactgagc aggaagtccc atattacgca
     6421 aaaaaactaa aacgacaaaa ggaatctctt ctggttgcta actaggaggg acatagatgc
     6481 taaaagcttt taaattccgt atgtatccga cagaagaaca aaaacagcaa ttgattcgta
     6541 cttttggatg tgctcgattt acctataacc atttgctgaa aaagcggcag aaaagttggc
     6601 aacaaacagg agtagccaat ttttcgctca cacccgctac attaaaaaaa gaatacccat
     6661 tcttaaaaga agtagacagt cttgctttag caaatgcaca gcttaatttg gatcgggctt
     6721 ttcgcaatta tttcaaggga cgtgccagct tcccaaagct taaaacgaaa aaaagtatgt
     6781 ggcaatctta tacgaccaac aaccaaaccc gtacgatcta tctggaaaat ggctacttaa
     6841 agttaccaaa acaaaaagaa ctaatcaaga taaatcagca tcggccagtt gaaggatcga
     6901 ttcgctccgc tacgatttct gctcgatata atgaagaatt ttatgtcgca ctactttgtg
     6961 atgtttcgcc agtaaaaaaa gaatctttag ctaaatggat cgggatcgcc tatcatccga
     7021 agacattgat ccaaacatct cgacctctag aagtaacttt accaaaattt caccagacag
     7081 aagaaaagct gcaacatgca cagcggaaat tgaatgtgaa agtccgctcc gctcaccata
     7141 gaaagatcag gttagatcag gcaagcaatt atcagaaaca aaagagaaaa gtaatggatc
     7201 tttatctgaa acaaaagaac cagcgagaag attatcttga acaattatct gggaaattgg
     7261 tcaaacaata tgactactta tttgtagaat cttttccaaa agaagaggca catgctgatt
     7321 tttctattca cgactggcat aaattaataa caaaattgcg ttataagtcc caatggtata
     7381 ataaaaaatt tcttttgatc aataccgacg gagcagaaga aagcaattcc gtaagaaaga
     7441 gccaagtagt agaaaaaatg ggcagacatt cggttatcaa gggataaaaa atgaatgaaa
     7501 ctgataggag cgttagggtt agccttgtta ataaaaagtc ttaatgactt ttgtttctag
     7561 gaatttttct tttgaagaaa agctcactaa ttatgacaaa ccaataaaac aatagcagaa
     7621 aaagttcgta cgagcttttt ctgctattgt taatttttta ttcagaaagt ttacgaatac
     7681 atcaaaattc tgaaacaaaa aacgtaactc acaaagagtt gtttttgcgt tttttcttga
     7741 atgattaggt ctgaatcttt tgctcacaac cttattcatg gtgccccggc ttgagctgat
     7801 agatttttga aggacggccg attcctacag gacgttcacc gatctcatcg aaatattgga
     7861 gcatagcttt cttaaaattg gaatgatcga tcgttttata atcgatccct aagaatttag
     7921 caaatacttt tcgagcttcc gtaatcgtga aatctttacc taaaacttgt agaacttgcg
     7981 gctcgtgttc cattttattg actacacggt tgaatgcttt caagatgatt tgactatggt
     8041 caaaagccaa agtatctttg ccagaagact ctccagtgac taaatcaagc gagatagcca
     8101 cttctccgct agataaatgg atcttgttac tatgacgttc tagtgtgaac cagcgtactt
     8161 ccttggcatc atctccggcg attagcggtt cttctccaat aaaggctaag tagctgactg
     8221 tgacgaccca gcctcgcgga tctcgattag gcgtactgaa tgtatgcagt tgctcaatat
     8281 tttcttttga aataaccaca ccagtttctt cctttgtttc tcttaagacg ctttctccag
     8341 ttgactcatc cttttggaca aagccaccag gcaatgccca tgaatttcga taaggatgcc
     8401 cttttctttg gatcaacaaa actttcagct gatcttcttc tttgttatag cacatcagta
     8461 cgatatcgac agtcaaagag ggcttgtcat attctggacg ttcctgtttt tgataccagt
     8521 taagaaattc ttcttcactt gctttatgtt cataatataa tttttcttct tctttagaag
     8581 caaaacggtt catctgacca cctccattga cagtaaaagt actttttacc ttattgtagc
     8641 agactctgaa aaaaaaccaa ggatttactt gaaaaatcta ggttatcatt taaatatgaa
     8701 aaataaatgg aagtatgaga ttgaataaaa agaaaagcag ataaaacggc aatactttat
     8761 accgctcttc acgaatggca aagaagccac aaataacgat atatacgtca atgataaaac
     8821 gcaaaatcca agctgctgtc ataaaactgt tcaaatgccc atggaggtta aaataaccat
     8881 acatacgaat ataaaaatag ctgaatatta aatgtgctaa tgaaagaaaa gcaatactaa
     8941 gtgtaaaaaa aagatgtttc ttctgcatga ttgttccagt cccttactaa gatagatagt
     9001 ctttattata ccacgtgtga ggggagaaat aaaaagccgt tttttagtag atatgaaaca
     9061 aaacaaaaac ccgcatttat tcaacctatt catcctttta aaggatagat tgaacgtaga
     9121 tagaagtcgt ttatccagtc tttttcattt tgaaaaaaag ctaacagcat gcttttttta
     9181 tggtatacta gcaaggaaac gaattcgcat catgcgataa aggaggaaga gccccgtggc
     9241 agaaaaggaa actttttata tcactacgcc aatctattat ccaagcggca aacttcatat
     9301 cggcaactcg tatactacga ttgcatgtga cgcaattgct cgttataaac ggttgatggg
     9361 ctttgatgtg ttttatctca ctggagtgga tgaacatggt caaaaaatcg aaacaaaagc
     9421 agaagaacta ggtgtaaaac cgcaagaata tgtagataaa atggcggctg atgtcaagaa
     9481 attatggaaa acgcttgata tcagttatga taaattcatt cgcacgacag atgattacca
     9541 taaagcagct gttcaaaaaa tatttgatcg tttattagaa cagggagata tttacttagg
     9601 tgaatatgaa ggctggtact ctgtttctga cgaagaattc tttacagaaa cacaacttgc
     9661 agaagtgtac aaagatgacg aaggtaacgt gatcggtggt aaagcgccaa gtggacacga
     9721 agtagaattg gtaaaagaag aatcatactt cttccgcatg agcaaatacg cagaccgtct
     9781 attggaatac tatgaagaac atcccgaatt catccagcca gaatctcgta aaaacgaaat
     9841 gatcaacaac tttatcaaac caggattaga ggatcttgca gtttcacgaa caacttttac
     9901 ttggggagta ccagtcaaga gtgatccgaa acatgtggtg tatgtatgga tcgatgcatt
     9961 atccaattat attactgcac taggatatgg ctccgaagat gaaagtctat tcgaaaaata
    10021 ctggcctgca gatgttcaga tggtcggtaa agaaatcgtt cgctttcata cgatctattg
    10081 gccaatcatg ctgatggccc ttgatttgcc tctacctaaa aaagtatttg ggcatggctg
    10141 gctattgatg aaagacggaa aaatgtctaa atcaaaagga aatgtcgttt atcctgaaat
    10201 gcttgtggaa cgttatggtt tagatgcact ccgttattat ttgcttcgtg ctgttccgtt
    10261 tgggtcagat ggtgtcttta cgccagaaga ttttgtttcc cgtgtcaatt atgatctggc
    10321 aaatgatcta gggaacttat tgaatcgaac gattgctatg atcaacaaat attgtgatgg
    10381 actcgttcct gactatgcct cacttgtcac tccatttgat agtgaactgt ctacgacggc
    10441 tgctaacgtt gtcggacgtt accatgatgc aatggaaaaa atggaattca atacagcatt
    10501 ggctgaaatc tgggtgctga tttctcgtgc aaacaaatac attgatgaaa cagaaccatg
    10561 ggtattagca aaagacgaag aaaagaaagc tgaattgaat agtgtcatga tccatttagc
    10621 tgaatctttg cgtatcgcag cgatattact tcagccaatc atgacagaaa cgccagagaa
    10681 gatcttcaat caactaggat tggactctga aacaatgaat cttgaaggat tacactttgg
    10741 cgaatttcct tcaggaacaa aagtagttgc aaaaggtaca ccaatcttcc cacgtttaga
    10801 tatggaagaa gaagttgctt ttattcaaga aaaaatgtct gaaggtactc aaacaaacga
    10861 agatacagtc aaatgggatc cagaagaaac agaacttgtt tcaacgaaag aaaaacaaat
    10921 caaatttgat gtatttgaaa aagtagaact aaaagtagca gaagtcatca actgtcaaaa
    10981 agtagaaggg gcagataagt tgctgcaatt ccgactagat gctggagaca gccaagaccg
    11041 tcaaatcttg tcaggaatcg ctgaatttta tcctgatcca agcgaattga tcgggaaaaa
    11101 agtcgtcatt gtagcaaatc tgaaaccacg aaagatgcgt ggacaaatca gtcaaggaat
    11161 gatcctttct gcagaagcac cagatggctc tttacaagta atcgaagcac caaaatccat
    11221 gccaaatggc tcagaaatcg catagaaatt caaagggggt atgacactag ttgtgtcata
    11281 cccccttttt ccgaaatttg agaagcaatt ttcgtaaatt ccttcttatt acttgaagcc
    11341 aatcatataa tacagaaaca gagaactatc caaaataaat cctagaaaaa gaagtacgag
    11401 cttcgctgat ttttgttaat ttagaataat actaatagga atcgtaaaaa cagaaaaaaa
    11461 gcataatgat aaagggtcgg gcagatttta attgattcat ctggtactct tttttattgt
    11521 ccttacattc ataaagtttg aatgcaatga ctttattaca tctatattta tatctaacag
    11581 tagcgttgat gtgttaatta taagttctat cactttatgt tcaattagtt tgtgctctta
    11641 tatgtgtgat tgaaaatata gataaaaaaa caatcatcgg ctttgacttc gatgattgtt
    11701 ttctttattt tgtaaaaatc ctatcacttg attttatggt aaccttttat ttttgtcttc
    11761 aaacggcttg gtaagagagc agtaagcagt gcaaatggga tcgaaaagac ataccagaaa
    11821 atcgtacgtc cgataccata atgttgatag agctttgtca acatcacgtg taacaagatc
    11881 aaccctaaaa gcaaggagcc agctataaac caatgattat cccaatgcat gacttcacta
    11941 agtgccatca ctccaaaagg atagaaataa aaatagatcc cgactaaggt catccgccag
    12001 ttctttgtaa atgttccgat attcaataaa aagaaaatcg ctgaaagcaa aataccgata
    12061 ggcggaaaaa gcccaaccaa aaaggaatac aacatccccc acaagatcat cacaggacct
    12121 ttgatcaatg caacgattgc gatgaacaaa gtcagcaata ttagtttttg tgataacgtt
    12181 gcaacagtca ttaatccaaa taaaattaat gtgaaccaat gccaatggga ctgctgttgt
    12241 tctttacggg aaaaatcgac ccggttggat aaataatctg ctaatttcgg ataagaacgc
    12301 ttaaaatcca taaactgttc ctccttcgtg gaaaaggtag gtttagtata cgaaaaataa
    12361 aaaagaaata catccgtctt aagagggagt ttttcactga aagtggagaa aaaagaatgg
    12421 taaattaaaa tattctcagt tatactgaaa attataccaa atttcaaaga agaaagagga
    12481 tgggaatgaa aggcaaacta aaacgagaaa tcaatctgtt tggcgcatta gctaccgtta
    12541 tgggaaccgt gatcggtgca ggcgtcttct tcaaaactgc tgctgttact gctagtactc
    12601 agtctacaag tttgacattg ctagcttggc ttttgggtgg tttcttaacg atctgtgctg
    12661 gattgacagt ggctgaatta gctacagcaa tcccggaaac tggtggagct gtaaaatata
    12721 ttgaagctgc gtatggaaaa ttgcccagct ttttattagg atgggcgcaa agcttgattt
    12781 actttcctgc taatattgct gcattatcga ttatctttgc gacacaattg acgaacttat
    12841 tacaattatc taccgattat cttttgttga ttgcagtgat caccgctgtt tctgtgacag
    12901 ggttgaattt gttagggaca aaagtcggtg cttctgtaca atcagtgacg ttgattgtta
    12961 aattaatacc aattgctgtg attgtcatat ggggattatt gaccccagga caaggaactg
    13021 tccagttatt tccaatcgaa gctggaaaag atgttacttt tgtcgaagga ttgagcagtg
    13081 cgttgttagc aacgttgttt gcttatgacg gctggttagg tgtcggtgca atggcagggg
    13141 aaatgaaacg gccggaaaaa gatttgccta aagcaattat tcttggtttg agttttgtaa
    13201 cagtcgttta tctattgatc aattttgttt ttttgaaaac tttgccgatt gatcatcttg
    13261 ccggtaattt gaatgcagct tctgaagcat cagacgtcat ttttggagga atcggtggaa
    13321 agctggtcac tattgggatc ttgatctcgg tatacggtgc attgaacggt tatacactaa
    13381 ctgggatccg cgtgccttat gcgatggctt tagaagacga gctacctttc agtaaacagc
    13441 tgacaaagct ttcaaagaag tttacggttc cttatgttcc tgcggttttc cagttagcag
    13501 tagcttgtat tatgatgagc cttggttctt tcgacttttt aacagacatg ttgatttttg
    13561 tgatgtggct attcagttta ctgattttta tcggtgtctt tattttaaga aaaaaagcgc
    13621 cggaattgcc acgtccatat aaagtaccac tttatccaat cgttccgatt attgcgattc
    13681 tcggtgctat tttcatttta ggtatgacga tgatgacgca aacgaaatta gcattgatcg
    13741 gtataggtgt taccttgata ggtatcccag tctattatca aaaaaagaaa aaacgctcag
    13801 aagagtaaaa tatttcaaca acagacagaa aaagtacgaa ctaggtcctc tagatcttcg
    13861 tgctttttct atttcaataa aacaagttct tctattttga tttttgctac ttctgcaagt
    13921 agtgcttatg tgctaaagta agaatgaaaa ggagagataa acatgatatt tgattctcat
    13981 acccatttaa atgctgaaca gttcaatgaa gatattccag aaacgattca gcgagcacaa
    14041 gaaatggggg taacgaaaat ggcggtcgtg ggcttcgata cgccaacgat tgaaaagtct
    14101 ttgcaattaa gccatgacta tccaaatata tacagtatta tcggctggca tccgacagag
    14161 gctggcagtt atacgaaaga aatcgaaaat aaattacaag aacaattgac tttaccaaag
    14221 gttgtagcat taggggaaat tggcttggat tatcattgga tggaagaccc gaaagaagtc
    14281 caagataaag tatttcgcag acagattgct atcgcaaaag aaatgaacct accaatcagt
    14341 attcatacta gagaagcact tgaagacacg tatcaaatcc taaaagaaga agatgttcga
    14401 gatatcggtg gaatcatgca tagtttcagt ggtgattatg aatgggcaaa acgatttctt
    14461 gacttaggga tgcatatttc atttagcggt gtcgtcactt tcaaaaaagc attagacgtc
    14521 caagaggctg cagcaaatgt accaatcgaa cgattacttg tggaaacaga tgccccttat
    14581 ctggcaccag ttccttttag aggaaagcga aatgaaccag gctatactcg ttatacagta
    14641 gaaaagattg ctgaacttcg gaatcttccg acagaagaag tggctgccca aacatggaca
    14701 aatgcgcacc gattatttgg tctaagcgaa catggataag atgaagatcc aagagattat
    14761 agtcgttgaa ggaaaagacg atacaagaag gctgcgggaa gtagttgatg cagatacgat
    14821 agaaacaata gggtctgcaa tcaatgaaga gatcctgatg caaattgaac atgcccaaga
    14881 gactcgtgga gtgatcgttt ttaccgatcc tgatttttca ggagaaaaaa taagaaaaat
    14941 cattatggaa gctgttcctc aggcaaaaca tgcttttttg ccgcgcacac aagctaaacc
    15001 aaagaaaaag ggaagccttg gtgtcgagca tgcaagtgat gctgctattt tagaagcatt
    15061 gaaaaaggtc gtgacgccag acatttccca aacggctgtt tctgaaatca ctagacaaga
    15121 ccttttagac tatggtttaa tagctggggc acaagcaaaa gaaaaacgag aaaaactagg
    15181 tgacgagttg cgcatcggtt acacgaacgg caaacaattg gaaaaacgtt tgaagatgtt
    15241 tcgtattacg ccgaaagaac tcgcccaagc aatgaaaata gtggaggaac aacatgagtg
    15301 aatatcgtga aattgcgaca ccctctagaa caaaagagat tttattgaaa catggttttt
    15361 catttaaaaa aagtttagga caaaattttc tgactgaacc aaatatttta aggaaaatcg
    15421 tagaaactgc gcaaatagat gaacatacga acgtgatcga agttggacca ggaatcggtg
    15481 cattgacgga acaattagcc aagcatgcaa aacaagtcgt agcatttgag atcgatgatc
    15541 gtttgatccc tgtattggct gatacgatgc aaccctatga caatgtgact attgtacatc
    15601 aagatatttt aaagaccgat ttgtcaacag ctgttagaga aacatttcat gaggagcttc
    15661 cgttaaaagt cgtagcgaat ttaccttact atattacgac accgatcatg atgcattttc
    15721 ttgaatcaga tttggttgtg gatgaactag tcgtcatgat gcaaaaagaa gtagctgatc
    15781 gtatctctgc tgaaccagga acgaaagctt atggttcatt atcgattgct gttcaatatt
    15841 atatggaagc cagtttagca ttcatcgtac caaaaacagt atttgtccct cagccaaatg
    15901 tagattcagc gattttgaaa ctgacaagaa gagatatacc ggcagtcgaa gtgacagacg
    15961 aaaaagcatt tttccgattg acaaaagcgg catttcagca aagaagaaaa acgctttgga
    16021 ataacttgca gcattcgtat ggcaaagatg accagacaaa agcatggtta gcaaaaagct
    16081 tggaaacagc tggaatcgat ccaaaacgtc gtggggagac tttgtcatta caagaattcg
    16141 ccgcattaag caatgcgatg tcagaaaata tgcagtaaaa aaagaggttg tgacagaagt
    16201 tgtgaaaaag ctgtcctgca tcaagtaata agaacagcca aacaaaaata gttcctcatg
    16261 tttttcgttt gtttgggctt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    16321 aggttgtggg cctgccgttt aactctaagt cacaaggaac aatttcaaaa aacagcttct
    16381 catgtttttg agttagttgg tcttattact tgatgcaagc ttttgaccac tattatgccg
    16441 aacaaggaga tgtgacacaa attttgtcac atctcctatt tttgtttctt gatcaaaaaa
    16501 ttcttatact tgatactcac actcattttg tatggattta gaaaagaata tgtgtcttga
    16561 cagttattag taagtgagtt atactaagtc gacatttatg tgtcaagaca tattaggaga
    16621 gagaaaaaat gagaaaaaat tcagttcgaa aactaacgat ttctgcttta ttgattggga
    16681 tgggggtcat tatcccaatg gtcatgccta aaatcatgat tccaccagct tcctttactt
    16741 tggcgtctca tgtgccatta tttattgcga tgttttttac accaggagtt gcagtggctg
    16801 ttgcgctagg tacgacattt ggtttctttt taacaacgcc ggttatcatc gcattgcgag
    16861 cattgtcaca tcttgtgttt gcgttgattg gggcttggta cttgcaaaag catccgagta
    16921 tcgttttaaa aaatggacag tttacttttg ctaattggcg tttccaaggg ttcaattttg
    16981 tgatcggtgt gattcattct ttagcagaaa tgctagtagt cagcatcttt tactttattg
    17041 gtaatatgcc tgaaacatat tattctcaag gatattttta tacagtattc atcttgatgg
    17101 ggattggtgg gcttatccac agtttagttg attataatat tgcttatttt atagcaggaa
    17161 cactcagcaa atattttgat atccccgtat ttacggcagc aaaaaaagaa aatcggaaag
    17221 agtcgattca agggtacgcg aagaaaacga catattagac atgatctctc aaaaaaaaaa
    17281 ctctcatcta gtaatggata ataaatcctc taagatgaga gttttttgtc ctaaaaagag
    17341 ctgcaccaac atcttctggc acagctctaa cagggggcaa tttatggaag tttgatgact
    17401 tgttctgttt ctttatcaaa gaagtggcct ttgctgatgt tgaaggcaag gtcgactatt
    17461 tcgcctggtt tatggaaatc tcttgcatct acttttgaga tgaactcagt agaaccagtt
    17521 tttgtgtaaa gcatcgtttc agctccaagt aattcggaaa cgacaacttc tgatttcact
    17581 acggcttcag gtgaagcttc aagggctact tgttcactgt ggatgtcttc tggacgaata
    17641 ccgaagataa cttcttttcc ttcataacct ttttcaacta atactttgtt ttttccttca
    17701 ggaatacgaa gcttaagccc atgtccatct gtgataacac catttttcaa tgtcacatta
    17761 aagaagttca tggcaggaga tccaataaag ccggcaacga acatattgac gggtgtatca
    17821 taaacttctt tcggtgtacc gatttgttgg ataaatccgt ctttcatgat aacgattcgg
    17881 tcagccatag tcattgcttc tgtctgatcg tgagtaacat agatggtcgt tgtttcaaga
    17941 cgctggtgaa gtttggcaat ctcagcacgc atggctacac gtaatttagc atctaaattt
    18001 gataatggct catccattaa gaagactttt gcatcccgaa cgattgcccg tcccaatgcc
    18061 acacgttggc gttgtccacc agataaagca gctggttttc gtttcaagta ttctgtcaga
    18121 cctaagatct ctgctgcatt gtccacacgt tttttgatgt ctgctttatc atatttacgt
    18181 aatttcaagc caaaagccat attatcaaaa acagtcatat gaggatacaa tgcgtagttt
    18241 tggaaaacca tggcaatatc acggtcttta ggtgccacgt cattcatgac ttttccgccg
    18301 atatttagtt ctccttcaga gatatcttcc agtcccgcga tcattcgcaa agtagttgac
    18361 tttccacaac cggaaggacc aacaaaaacg ataaattcac ggtctttgat tgatagatta
    18421 aaatcagtta cagaataatt ttctgcattg tcatattttt tatatacgtt tttcagagcc
    18481 atttctacca taatacgttc cacactttct tttttagttt tattaactat ttaaagtata
    18541 aatgaaagcg ttaactaact tcaatgtcat gttgaacaag aattttttct tttttttgtc
    18601 aatgtgctag ttttaagctt taataaatat ccataagaaa taaaaactga tacactggag
    18661 taaataagta tgttaacaat atcaaaagga gtgtctctta tgaaaatcgc tttgatcgct
    18721 catgaccgaa aaaaacctat gatggtgaaa ttagcaacag cgtataaaac aatattggaa
    18781 aaacatgaac tttttgctac cgggacgact gggcagagga tcacggaagc aaccggcttg
    18841 ccgatccatc ggtttaaatc cggaccgtta ggcggggatc agcaaattgg agccatgatt
    18901 tcggaggatc agttagattt ggtgattttt cttcgagacc cgctggctgc acagccccat
    18961 gaaccagatg taacagcact tatccgttta tctgatgtct atgagattcc gttagctacg
    19021 aatatcggaa cagctgaggt gctactaaga ggactggaag ccggcttcct agattttcga
    19081 agaatcgtcc atgaaataga caagcatcca ctttcatttt gatggataag cgtatggaaa
    19141 aataacgttt aatgttcgcc tatatgtagc tataaaagct aaacaaactg ataatatgcg
    19201 atataatacg aacgtattgg ataatataaa caataatatt agtttttcgt atttgcagta
    19261 aatgtgaaaa tatgctatag tttctgtggg aaaagatccc aaaaataata cagagtagaa
    19321 aataaagcgt taagtgctaa gaggatggga tgttgcctct tggacgaagg acgtaatgtt
    19381 cgcggtttta ttttcgcatt cgctgcatga tacgatgtag cagctcttaa aggagatgct
    19441 aaaaatgaag aaacaccggt tagacgcacg tatgattgct attatgggct tattgattgc
    19501 attgatggtc actttgtccc gactggtagc aatcgagacc ccatttatta aaatcagtgt
    19561 cacatttatt ccacaagtga tcatgggcat tctttttggt cctttttggt cagggatagg
    19621 cgcagtttta gctgatttag taggaatggc cctcttctcg aaatctgcat tctttattgg
    19681 atttacattg aatgcattta ttgaaggagc aatctacgga ttctttttct atcgaaaaga
    19741 gatcacttgg aaaaatgcca ttctggctac gcttagtgtc acgctgatca tcaacttatt
    19801 tttgacacca ttatggcttg cgttgatgta ccatgtgcca ttgttcagct gggtcgtttg
    19861 ggcgccccgc ctgttaaaaa cagtaatatg gttaccgatt caatcaattg ccatttatta
    19921 cgtaggacgt agtattcctt acaaaaaaat tttaagaagt ttagcgatcc acgctaaata
    19981 aaaaacaagc agtcttccca tcccagaaga ctgcttgttt ttattgtgct gtgaattgtg
    20041 cttgatagag ccgtcggtaa taaccatctt tttgaagcag ctcatcatgg gtgcccattt
    20101 cgacaatagc tccctgatcc atcactaata tctggtccgc atttttgatt gtggacaggc
    20161 gatgggcaat cacaaaactg gtttttcctt ccatcattgc taagaaggct tgttggattt
    20221 ttttctctgt caatgtatcg actgagctgg tagcttcatc taagatcaac atagggggat
    20281 tactgatcat cgttcgagca atcgtcaaca gttggcgttg tccatctgat atctttaacc
    20341 cttgaccgcc aatcttagta tctaattttt ccggcaaacg ttctacaaat tcatacatat
    20401 aagaggcttt tagggcatca ttgatttctg cgtcacttgc ttccggattc ccaaaacgaa
    20461 gattttctcg caatgtactg tcaaatagcc aagtatcttg taacaccatt ccaaaacttt
    20521 tacgtaatgt atcacgttgg atagagcgga tgtcatggtc atcgactgta attgttcctt
    20581 ttgttgtgtc ataaaaacgc atcagcaaat taacaagtgt ggattttccg gcccctgttt
    20641 ttcctactat agcaatggtt tgacctggat aagcagtaaa attgaaatct tcgatcaaac
    20701 gctgcttggg gtcataagaa aaagaaacat gatcaaaaga gacttttcct ttgatatttt
    20761 tgggttccaa ggcataggca tctggcaaat caggcttttc ttctggctga tcgatcaaat
    20821 caaatgctcg ttctagacca gaaaaagcgg tttgcagttg tgtcgtgatt ccagaaagtt
    20881 cgataaatgg tttagaaaac tgacttgagt agatcgtaaa gctggaaatc acaccaacag
    20941 tgattgacga accactcttc aatgccaata ggccgccaac taaaccaatc gataaataag
    21001 caaggtgatc tacaaaacga gaagacgggt ttgtcaagga agaagaaaat tgtgcccgtt
    21061 gtcctttttc atatagatca gcatttagca gttcaaattg ctgttgacta gcttcttctt
    21121 gctgaaaagc tttcacgatt ttctggttac cgattcgttc ggtcacaaag cccgagatat
    21181 ccccaataat cctttgctgt ctatcgaaat ttgtttgtga agcacgagca acgatccaac
    21241 tcacaaggaa aataattggt gtagaacaaa gaacgaccaa tgtcagaagt ggacttagac
    21301 gcatcataaa aataaacgaa agaatgatga ttgccactcc agaaaataat tgattgaagg
    21361 ccgcagaaat tgcaatggaa atattgtcca tatcatttgt aaatcgactg acgatatttc
    21421 cgtgggagtt ttgatcataa taacgaagtg gcaatcgatt cagatgagca aatgcatctt
    21481 ttcttaattt tgcaacggat aagtaagcta accgattgct taacacttgg atgagccatt
    21541 gactgactac gttgattaaa acgataccac cgaatagaat aagtaaatgg aacagtcgat
    21601 tgaagcggac ctgtccttct ccaatcattt gatcgatact ttgaccaatt tgtaacgtca
    21661 tcacaactgt tgcaatccca ttgatgattc ctagtatcac agctagccaa atgtcttttg
    21721 cataggcagt aaggtaagga atgaatcggc ggagcgtctg caaggataat ttttttactt
    21781 tcataactat tcttcctcct gtgatgcgac gatttcctga tattctgctg attgagcaag
    21841 taattcttca tgggtaccct gagcagcaat cttaccttga tccatcacta agatatgatg
    21901 tgcttcttga atagagcgga cacgttggga gataataatc actgtactgc caagataagt
    21961 ctgtagcgcc ttacgcaaat ccaagtctgt ttgataatcc aacgcactta aagagtcatc
    22021 taaaatcagt aatgctggct tggcaatcaa agctcttgca atcgtcagtc gctgtttttg
    22081 tccgccagag aaatttttcc cattttccat gacttttgta tctaaaccat ttggaagctg
    22141 acggacaaat tcttctgcct gagcgatttt cagagcttcc caacattctt tttctgtggc
    22201 gtctttctta ccccattgaa ggttttcacg aatagtcccg gtaaatagta cagaagtttg
    22261 gggaacaaga gcaatttgct gacggagatg atccagctgc caagaacgaa catccattcc
    22321 gtccattata actgtaccgg aactgacatc atagaaacgc ggaatcaatt gtgtcaaagt
    22381 cgttttcccg cttccggttg gaccaatgat tccaaggact gtatttcgct tcagctgaaa
    22441 tgtaatattt tggagagaaa gaccagaatc aggagtataa cggaagtcaa catggtcaaa
    22501 agcgataatt gcttcagaag aaaaatcggg agtagtactg acctcagctt caagaatcga
    22561 attttctgtg tctagcacct ctttcacccg atttcctgaa gcttcggcac gtgtgaagat
    22621 cacgacgagg tttgaaacaa cgatcagtgc caaaagcatt tgattcatat aattgatcaa
    22681 agcaagaact tgtccttgtt gcaagctacc aatatttact ttgatcccgc caactgtcag
    22741 caaacagatg atcccgacat tcatgatcaa ggttgtagca ggtgataata gggcagagat
    22801 attggaaaca cgtaaataat tgttcgctag atcatttgtg cttttatcga acttctctgt
    22861 ttcttttttt gtctgcgcaa atgcgcggat gacacgaaca ccgctaagat tctgactaac
    22921 gtgttcattg agctgatcta gtttttcttg cactttttgg tagagaggaa cggttttttt
    22981 gatgataaaa aataaaatga ttgagaaaat aggcaataat gctaagaaaa acagacctac
    23041 ttcccagtcg atcacaaaag ccatgaccac tgacccgata cttaaaaacg gtgctctgat
    23101 caataaacga atgaccattg ctaaagctag ctgaagctga ttgatgtcgt ttgtcattcg
    23161 ggtgatcaag gtatctgttc caaaatgatt caactcgttg tgagagaaag tattgatttt
    23221 tttgattagc tgattacgta actctgtacc aaatccttgt gaagctactg aagcatagta
    23281 ttggcaaatg atagcacaaa tcagaccaat cacagacata gcgaccatcc ataagcccat
    23341 ttcaatgatt tttcctcggt cattcatttt tagtccatta tcgatcaagg aagccatcat
    23401 taaaggcagg acaagttcga agacagcttc aagaaatttg aagaacgggc ccaaaatgat
    23461 ttgcttacga tagtcttttg cataacgtaa taactgaatc atatgattcc tcctattatt
    23521 tatttgtcca caattgaaaa aacaagtact accacattgt agcttaggaa atttagaatg
    23581 aaaagagaac aagccttttc ataaatgaaa gttttattat gaagaagctt tagaaattga
    23641 atagactatt ttttttaaaa aaaagaaaat gagaaaacat gccagtctct tcattcatag
    23701 gaacgtctat tttacttaat ttttctataa atgaattctg cataaaaaat atatcaagta
    23761 aatgtttata gatgaaactc tatttacttc ttttttaatt tgttcttgca atcttttttg
    23821 ctacgtaatt tttgtaaaaa agtgggttga gagaaatcta attgattttc aaagaaggag
    23881 tatgaaaatg agaaaaatat ttattcgttt attgtgggta ctatcttaga ggaataaata
    23941 atagtggaga aaactatggt ttggcgcaga ttctatcagg tttgatggaa cgaagacaac
    24001 ctcccgacat caagtcaaaa gccacttgag attttgatct gaaaataaaa aaagaaaaca
    24061 aggtaggaac atcaatgttt ctaccttgtt ttcgtaatca atgggaaaaa actaagagtg
    24121 gtgtacgtaa tcaataaaaa ggatggtgac aacagtacct ctttactctc tgaaagcatc
    24181 ataactcgtt tttcctcatt atgccaataa attgttgtta taccacatat aaggatttgt
    24241 aatagctaaa aacagacagg aaagttgaaa tctgtgaatc ttattttgta tggaaggatt
    24301 tatagaacgc aaaaatcaag ataaagatga ataaaaatcc tgtttcggct atatattgcg
    24361 tatctacgcc aataaaacga gtttgttctg tcatagccat ataaagcaaa ggcaaaaata
    24421 caactacagc gagataagat ccgatgattt ctcgaaaagt tttccaggaa gtatgtttcc
    24481 tgtttttata tttatccgat ttcattgaat agtcatagcc tccttattct ttcatatgtt
    24541 gaaattccag tataaaaact ggaaggaact gtacgttttt tagaataaaa attataaggg
    24601 ataagtaggc tgctgtctat aataagatat atttttcaat tctttattaa agattttatt
    24661 gacaaaatga atccgttttc tatgatggag gaggggtgaa taagttgttg aagaaggaaa
    24721 ttttatgtta cttagagaac cagacagcct ttttcgattt agaacatatg aacgaggtct
    24781 ttacagcaca aaggttagct gatcattttg gggtaaaaag aaacacagtc agtcattatt
    24841 tgaaccagtt aaatgatgag caactactag tcaaaatcaa ttctcgacca gtgatttatt
    24901 tccacaaaga agcttttgaa aaacaatttt ttaagttacg cacgaatttt tatttttcta
    24961 tcgatgatct caaagaggaa cagccttttt tcgcgcaacc aaaagactta ttctctttta
    25021 tgatcggata cgacgccagt ttgaaagaaa gtatcgaaca aatcaaaaca gctctttact
    25081 atccagatgg cggattacca cttttgatta caggcgaaag tggcacaggt aaaagttatt
    25141 tagttaattt ggtctatcag tattgtctgc ttcatgatct gctagaagat tctgcaccat
    25201 tcatcacttt taattgtgca caatatgcag ataatcctga acttttgact agcaatttgt
    25261 ttggacatgt taagggagcg tacactggtg cagaagaaaa taaaaaaggg gcttttgaag
    25321 cagcagatgg tgggatcttg tttttagacg aggtacatcg cttaacacca gaaggtcagg
    25381 aaaaactgtt cacctatctt gatcaaggtg tgatttatcg tatgggagaa cctaatttgt
    25441 cacgccggat caaaacacgt ctcttttttg ctacgactga agagattact agtcatttct
    25501 taacaacttt cattcgaaga atacctatcc gaatcgaact acctgcactt aatcagcgta
    25561 gtcgtaatga acggctagaa ctgatttact ctttttttct cgaagaacaa agaaaaatgg
    25621 ggagcccttt gtcagtcagt ggaccagctt tgtcactttt aacaagtcag aaattatccg
    25681 gaaacatagg agagctgaaa aatatcgtca aagtttcagc agcaaaagca tatgctgaac
    25741 agcgtgaaca gcctgaaatc catgtcacga tccatcattt gcaaaaagaa ctacttgcgc
    25801 agcctattac taaaaataca gcacagcaag gaatctatat tacgcaagac cagaccttag
    25861 agcaattaat ggagaagcaa cagcccgaac agcagcgaat cgttaaaagt tttgagcaaa
    25921 tcctaattga tttcaagaaa aataactgtc tgctatattc ttgcgaagga aaattaaagc
    25981 aagaagtgat tcaattattt gattttttgc tgtttgagac cagtagacaa gaaaaacacg
    26041 agctgctggt ttattggaca caatctatcc gagagacctt tcgacaaatg gaaactgcct
    26101 atcaaatcaa attcgatgga aacagtgtct atgccttgag ttactatctc tatcagcgaa
    26161 gcactatcaa atggttaccg gaagaacatg atattatcca actaatcaaa gaattagaac
    26221 aacaagtttc tcagtcttat ccatctagtt atcactatgt ccaacgaatt ctggaattaa
    26281 gtaagatcaa attagatatt gaagacacaa gtatggatcg gattttactg acgatctatc
    26341 taaaaaaagc gaaatggtca aaagaaaaac gactgccaaa agcgatgatt gctgctcacg
    26401 gttatgcaac tgctagcagt atggcaaatg tagtaaatcg tttattacaa gaggatctat
    26461 ttgaatcatt tgatatgcca ctagatgtca caccacagca gattgcttca gaaatccttg
    26521 attattgcga aaacagcgat gtttcaaatg gattgatcat attagtagac atgggatctc
    26581 tgaaagaaat ccatcagttt tttaaaaaac agttatccgt accgcttttg attttgaata
    26641 atgtgacgac acctttagca atcacagtag gggaatgcct gcaaaaaaat gtatcattag
    26701 aagagatagc agaagagact gttcgacaga tccagccaga atggcgactg ctctatccag
    26761 aggaaaacaa acccaaagca ctcattacta cttgcttcac tggaatcggt acggcggtcc
    26821 acttaagtga actgctagaa aaaagcttac ctacggcttg tcagttgaaa atcatcccat
    26881 atgaatatca gcaattaaaa gataagaaaa acagtgaccc attattttcc atatacgaag
    26941 tcgtaggaat gattggcacc acagatcctg atatcaccaa tatcgactat ctttcattag
    27001 aagacctcat ctcgggggaa aagatgtctg tactagctga atggttggac actactatga
    27061 atgcttcaga gaaggaaata ttcaatcagc gcatcattcg taatttttca ttggaaaaag
    27121 tactggattc cgtgactatt ctagatacag agaaggtgat gggagaaatc gatcatttta
    27181 tgagagaatt agaggtgcag ttgaatcgaa gcatcagcaa tcccaaaaaa ttagcgttgt
    27241 atgtccatgt gagctgtctc attgaacgat tgatccgaca aatgccaatc gaaacctatc
    27301 aaggattaga gaaatggaag cagtgtcaaa aagaagggtt atctgctatt aaatctgcgt
    27361 ttagtgtcat tgaagagcat tatagtgtca tgatccccaa ttctgagatt gcctatatct
    27421 atgatatttt gagtgaagat acagagtttc tctcgataga agaagaattt tgaacataag
    27481 caagttgttg ccactaattt tggcatgcaa cttgcttttt tagtatgtgt aagtgaaaag
    27541 aggagatgaa aaagtggata gagaaattat cttagcttcc catggaaaat ttgcttcagg
    27601 gatattagat tcattagagc tgatctatgg gaaaaatcat tctattactg tattcgattg
    27661 ctatactgat gagaattttg atttagcgga aacagtaagg cagcttttta tgaggtacaa
    27721 agataaagaa atcattgttc tgacggatct ttttggcgga agtgtcaaca acgaattctt
    27781 acaatatatc aatcgggatc atgtctatct cgttgccgga ttgaatttgc cactattgat
    27841 tgagctagta tctcggttgg acgctgaagt agagacaact gttttgatcc agcaggtgct
    27901 aactgattca aaaaaaatga tccagttttg taacgagcgg ctcaatcaga aaatagaaga
    27961 agacgaattt taggaggaag aaaaatgatt gtattgacaa gagtcgacca tcggttatta
    28021 cacggacaag tagctttttc atggacacaa acaataggcg cagactgtat tttgattgct
    28081 aatgatgatg tacctacgaa tgagatacga aagacaacga tcaaattagc caagccacaa
    28141 ggagtcaaac tagtgatcaa atcaattgat gactcgattg ccgcgttgaa aagcggggtg
    28201 acagataaat ataagttatt catcgtagtg gaatcgatcg aggatgcata taagctggca
    28261 gaagcgtatc cagaaatcaa aaccatcaac ttaggaggga tcaaggcaaa acctggtact
    28321 cgcagcatcg gaaaagcggt aaatatttta ccagaagagg aggaacgctt gaaacgatta
    28381 attgacaaac aaatagaagt agaaatccgt caagtaccag cagataaaaa agtggatgta
    28441 agagaagttt tgtaagaaag gaagaacctc aatgattgta caagcagtct tattaggaat
    28501 tgttgcattt gtcgcacaat cagaatatgc actagggaca tcgttgcttt ctcgtccgat
    28561 tgtgacgggg ctgtttacag gaatcgtgtt aggtgatgta aaaactggga tcattatggg
    28621 cgcaacttta gagttagcat ttatcggatc gttctcagta ggtgcttcga ttccacctga
    28681 tgttgtgacc ggtgggattt tagggacggc ttttgctatt acagctggag ccggtacaga
    28741 aaccgcgctt ttactaggtt tgccgattgc tacgttgaca ttgatattga aaaatattta
    28801 cttagggctt ttgattccaa ttatgaacca taaagcagac aattatgcag aagaaggaaa
    28861 ttataaagga gtcgagcata tgcacttgct tgccggtatc ggtctttcct tgatgcttgg
    28921 tgttgtggta atggtttctt atatggtggg aagcaatacg atcggaaatc tattaaatat
    28981 gattcctgac ttcgttcaaa aggggttgtc agtagctaca gggttgattc cagctcttgg
    29041 ttttgcgatg cttgctcgtt tgctgatcaa caaacaagtt gcaccatatt ttttcttagg
    29101 ttttgctata gcttcttact tagagattcc tgtgaccgga gtagctattt ttggtgcgat
    29161 tttagcagtt gtggtagtca atattatgaa tgtccgtcaa ctaccacttc aaacaactgc
    29221 aggggaggta gaagaagatg aagaattctg agatcaaaaa cagcaaaata acgaaaaaag
    29281 agctaaatca cgtattttgg cgttcttttc aaatggagtt ttcttggaat tatgaacgtc
    29341 agatgaatat ggcttatgtt tatgcaatga ttccgatttt gaaaaagtta tatcagtcga
    29401 aagaagaaat ggcagcagct ttgaaacggc atctagagtt ttttaacacg accccacata
    29461 tcgttacact gattttagga atcacggctg caatggagga agaaaataag gatgatcctg
    29521 aatttgatgt gactgcgatc gatagtatca aaacatcttt gatgggacct cttgcagggt
    29581 taggagattc tttcttttgg ggaactttgc gattgattgc cacaggtgtg ggtacctctt
    29641 tagccatgca aggaaatatt ttaggcccta tcttgttcat tttgattttc aatattccgc
    29701 acattctgtt tcgctatcta gcaacaggtt ggggatatcg cttaggaacg ggttttttga
    29761 aaaagatcca ggcaaacggt atgatggaaa gtttgacatt aggcgcgtct attattggac
    29821 tgatggtcgt tggagcaatg actgccacga tgatcaccat caatattcct ttaaaaatcg
    29881 gttcaggaga aaatgctgtg actgttcaaa gtatttttga tgatatcgtt ccaaatatct
    29941 tatgtcttgg agcatttggc atagtgtttt atctattgaa gaaagaagtg aaaccattaa
    30001 cgatcttgat cggactagct ttctttggaa tttttggttc gtggataggg attttttaaa
    30061 aaaggagcag agaaaatgtt tacgatgcaa gattatatct atgaagaaaa ggaagtacta
    30121 agtacgatct tgaaaaagaa tgatttttcg acaagagaac atatgaaaaa aacagtgaat
    30181 ttactgatct tagcaacggg ttcatcttat aacgcttgtt tagcagcgaa gccagcattg
    30241 gaatcttatg gtgatcttac tgtcgatatt caagaacctt tttatttttt caattatgga
    30301 aaactttctc cttcaatcga tactgtgatt gctgtgtcac aaagcgggaa aagtgcttct
    30361 actgttgaag cagtaaaaat gatacaaaaa caagggttac ctgttgttgc tatcacaaat
    30421 gacgtgcaaa gtccacttgc tttagaagct gcacaaatta ttgatttagg tgccggggta
    30481 gaatcagtag gcttcgttac aaaaggctat tctgcgactg ttttacagtt actactctta
    30541 gggataggaa ttggaataag taaaaataaa atcagcaaaa aaatagaaca agactacatg
    30601 caacagttgc gcaaaataat caaccattta ccagcgatta ttcaaaaaac ggaagaattt
    30661 tttgatggat atcaaagttt atttcgttta gcacagcgtt tcgtcaccat tggatatggt
    30721 ccaaactggg gaacagctaa agaagcagag acgaaattga ctgaaacaat ccgcgttccg
    30781 tcacaaggat ttgagttaga agcttatatg cacggcccat acctggaggc agatgcttcg
    30841 catttgctat tctttattga aggggaaagt gtaaataagg aacgttcgca aaagttgcaa
    30901 agatatatga gtcggtacgt tggagagact ctcaccatta ccacaaaaaa agcaagaaac
    30961 gagaaaacac ttggattagc aatagaatgt gatgaatatc tgtctgtatt agcgcttgtc
    31021 gttccgtttc aactattcgc ttataaaacc gcagtagcta aaggtattga cttgaacaaa
    31081 aagatttttg aagactttga tacagtcttg aaaagtaaat tataagaggt ggaaatcatg
    31141 ctgaaattca atgaagaaga acaaatcaaa gcaataaaag gcgcactagc tttacgtccg
    31201 caagtagaag aaatcatcga taaactttat gcggaaaaat ttgacgctgt ctattatcta
    31261 ggaatcggtg gaacgtatgc ttcctcgatg caagctgtca cttatatgaa tgggaaaagc
    31321 aacttgcctg tctttgtaca gcatgctgca gaatattaca ctactggaaa caaaagatta
    31381 acaaaagatt ctatcgttat tttgtcctct gttaccggta caactcaaga agtagtgaaa
    31441 gcagtagaac aaatcaagaa agtaggagcg acactgattg gatttatcga taaagcgaac
    31501 agtaagttgt ctcagctatg cgattttgtg gtcacttatc cagctcctgg aacagaacaa
    31561 atcaaatttt ttatggcagc agatcgattg atgtatttga atggagaatt cgaagcttat
    31621 tcagaatact atgaacagct cgaacgttat ttaccaacag ggttagtgga agcagagaaa
    31681 aaagcagatg catttggctt atcatttgca gaaaaacatc gacgtgatag tatgcattat
    31741 tttattggag cagggaatca atggggagct gtttattcat atgccatgtg ttactgggaa
    31801 gaacaaagct ggcttccatc gaaatctatc catgctgccg agtttcttca cgggacgtta
    31861 gagatcgtag aagaaacaac cccagtcaca ttatttttag gcgaagatga acagagagtg
    31921 ttatctgaac gtgtggctaa acttttgcca aaaatttgca gcaattatac attgatagac
    31981 acaaaagact atccagtaga aggtatcagt gaaaaatatc gcggacgtgt tttgtctttc
    32041 ttattgatgc atgtagtgac acaacgcatc gatgctcatg tggagcagtt gaactgccat
    32101 ccattagaga tacgccgcta ctatcgtcag tttgattatt aggatagact ggataaaaag
    32161 aatataaaaa ataaagagag cagcgataac tgctcccttt atttttttaa aatattggca
    32221 tcaattacgt tataaaacac attttttgca ttatctacag ttcaacttat aatctgttta
    32281 atgggacatg tgggtgccaa ccattttttg ttatgcaata gtactattta gttgttgcat
    32341 ggatccgtag taaaagtcca agtacaacga gttgcgccag tttacatagc gttttgtatc
    32401 cagaatgttg actaaaacaa aagttcctat gtcaaccgtc aacagagaca atctgtccgt
    32461 tgcaggacct gacagattta gaaatgttca atctgtctct tatgtatttt aaatcaatac
    32521 tcactaggag aagtttccta acctcctaat agtaatgctg gtagtaacat tgtcaaaact
    32581 gcttcctctt aggaacaagc aaagcataac gagtgtaaaa ataaaatgta gactattgta
    32641 acatatcatt gattatttca agtaaaaacg ataaaagaag taaacaaaca cttaaaaaca
    32701 atggaaatta gtcccaactt taagaaatgt atactataat ggaaattatc aattatttat
    32761 ttggaggaat atccatgaaa caaaaaatcg cagttcttgg tcctggatct tggggcactg
    32821 ctttagcaca aacattggca gaaaacgggc atgatgtccg tatttgggga aatgtacctg
    32881 aacagattga tgaaatcaat aagtatcata caaatcagca ttttttacct gatttgacga
    32941 tcccagaatc gattatcgga tataaagaat tggcagaagc agtggataac gcagatgcta
    33001 ttttatttgt tgttccaaca aaagcgattc gttcagtcgc acaggaactt attggtaaaa
    33061 tgaatactaa gccaatcatt atacatgcaa gtaaaggact agaacaagat acgcataaac
    33121 gaatctcaga agttttggct gaagaaattc cagaagaaaa aagacaggca attgttgtat
    33181 tatctggtcc tagccatgct gaggaagtgg cagtacatga tatcacaaca attacagctg
    33241 caagtattga tcaaaaagca gcagcctatg tacaaaaact gttcatgaat gattatttca
    33301 ggatttatac aaataatgat gtgattggcg tagaaactgg tgcagcactg aaaaatatca
    33361 ttgcaattgg tgccggcgct atccatggat taggattcgg agatgatgca aaagcagcaa
    33421 tcatgactcg aggattggca gaaatcagcc gtcttggtgt cgcgatgggt gccaatcctc
    33481 tgacatttat cggtttgagt ggtgtggggg acttgatcgt tacttgtaca agtgttcatt
    33541 ctcgcaattg gcgtgccggt aacttgttag gacagggaca taagctggaa gaagttctag
    33601 aaaacatggg aatggtcgtt gagggtgtgg caacgacaaa agcagcagta gagctagctc
    33661 aacagctagg tgttgagatg ccaattactc aaacaatcta taatgttttg tataatggag
    33721 aagatatcaa aaaagcagca aaagaaatca tgttacgtga cggaaaaatg gagaatgaat
    33781 tttctcttcg ttaaatagat gatcaatagt taatcgt
//
LOCUS       DASHHB010000029        33263 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.029, whole genome shotgun sequence.
ACCESSION   DASHHB010000029 DASHHB010000000
VERSION     DASHHB010000029.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 33263)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 33263)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..33263
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.029"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(41..229)
                     /locus_tag="U2K71_001526"
     CDS             complement(41..229)
                     /locus_tag="U2K71_001526"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HEM7315611.1"
                     /translation="MAKVCYFTGRKTKSGNNRSHAMNSTKRTVKPNLQKVRVMVDGKP
                     KKVWVSTRALKSGKVERV"
     gene            499..861
                     /locus_tag="U2K71_001527"
     CDS             499..861
                     /locus_tag="U2K71_001527"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM7315612.1"
                     /translation="MAVKIQTSAGTIEITNDVIATVVGGAATDVFGVVGMASKNQIKD
                     NITEILRKENYSRGVVVRQEDNGIAVDVYTIISYGTKISEVSRNVQEKVKYNLEALLG
                     VTANSVNVFVQGVRVLPD"
     gene            913..2595
                     /locus_tag="U2K71_001528"
     CDS             913..2595
                     /locus_tag="U2K71_001528"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HEM7315613.1"
                     /translation="MSVTEISASQFQEMVQAGANRLQVNAEYVNSLNVFPVPDGDTGT
                     NMNLSMTSGAKAVVDSTSEKVGELAAMLSKGLLMGARGNSGVILSQLFRGFSKQIPDV
                     VSLNASDLAAAFTHGVETAYKAVMKPVEGTILTVAREAAKAGEKKAKSTDDVVEVMTA
                     VVKGAKRALAKTPDLLPVLKEVGVVDSGGQGLLFVYEGFLSALNGEFQADETYEPSPA
                     EMDDMVNAEHHRSVQGQMATEDIHFGYCTEIMVKIGEGPTVDSTFDYEEFRNYLDGIG
                     DSLLVVNDDEIIKVHVHTENPGEVLNYGQKFGSLVKIKVDNMRLQHETILEHDEEVNE
                     FAKKETPRIPYGIVAIAAGKGVQELFESLGANYVISGGQTMNPSTEDIVKAIQEVNAD
                     KVIVLPNNKNIFMAADQAAEVADIPVAVVPSRTISQGLTAMLSFNDQASLEENKETMT
                     EMLSSVVSGQVTHAIRDTSIDGVEIKEGDFLGMIDGKILISNPDITATALSTLEQMIN
                     EDTEIVTILAGDEGTVEQANEFAEKLTEKHPDLEIEIHQGDQPVYPYLFSAE"
     gene            2712..4748
                     /gene="recG"
                     /locus_tag="U2K71_001529"
     CDS             2712..4748
                     /gene="recG"
                     /locus_tag="U2K71_001529"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HEM7315614.1"
                     /translation="MRSLDDPITMLAGVGEKRAASLASLGIQTIEDLLTYYPFRYEDI
                     QERNLNEIQDQEKVTLKGIVVSPPVMNRFGYKKSRLQFRMMQEHDVFNVSFFNQPYLK
                     EKVILSEEIAVYGKWDAKRKALNGMKILGSQTMDDFSPIYHVNKSIRQTTLVDLIKKG
                     FQEYGTLIEENLPNNLVEKYRLLDRASAVRSMHFPKDHEENHQAKRRVVFEEFFLFQM
                     RLQGLKKAEKAETNGLEILYDVQKLKEFTQKLPFELTNAQKRVTNEICRDLRSPQHMQ
                     RLLQGDVGSGKTVVAAIALYATVTAGFQGALMVPTEILAQQHMESLQQLFDPNEVTIA
                     LLTGSTKAKERRELLEKLEQGEINIVIGTHALIQDGVEFQNLGLVITDEQHRFGVNQR
                     KVLREKGWRPDVLFMTATPIPRTLAITAYGEMDVSIIDELPAGRIPIETRWVRTPQLD
                     SVLDWTKKELARGHQMYVICPLIEESEALDVKNAVEIYEKLRELFAPKYEVGLLHGKM
                     KNQEKEVIMEAFKDNQMQILVSTTVIEVGVNVPNATVMLIMDADRFGLAQLHQLRGRV
                     GRGSDASYCILVANPKNELGVERMKIMTETTNGFVLSEKDLELRGPGEVFGFRQSGLP
                     QFAIADLVTDGNVLEVAREEAAALWQIKDWQMLPEYQPLAQSLQIENQDNRYFD"
     gene            4797..5798
                     /gene="plsX"
                     /locus_tag="U2K71_001530"
     CDS             4797..5798
                     /gene="plsX"
                     /locus_tag="U2K71_001530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HEM7315615.1"
                     /translation="MKIAVDAMGGDHAPQAIVEGVALAQQDFPEVEFLLYGKEAEIKK
                     YLTNEKNITIIHTDEKINSDDEPVKAIRRKKTASMVLAAQAVKNGEADAVFSAGNTGA
                     LLAAGLFIVGRIKNIERPGLMSTLPVVGKEGAGFDMLDLGANAENKPEHLLQYGILGS
                     FYARKVRGIGRPRVALLNNGTEATKGSEVTKKAYELLQNETSLNFIGNVEARELLNGV
                     ADVVVTDGFTGNAVLKSIEGTAMNMMNLLKSAILNEGIKGKMGAMLLKDGLRSLKAEM
                     DYSKHGGAVLFGLKAPVIKTHGATGPDAVRYTIRQIHTMLETDVVGQLVEQFEKSEE"
     gene            5920..6162
                     /gene="acpP"
                     /locus_tag="U2K71_001531"
     CDS             5920..6162
                     /gene="acpP"
                     /locus_tag="U2K71_001531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HEM7315616.1"
                     /translation="MTREEVFNKVAKIISNHFEIDTDKVTDELNIKDDLKADSISIME
                     FVLELEDEFGTEISDEDAEQIETVGGAVDYISSHLK"
     gene            6430..7434
                     /locus_tag="U2K71_001532"
     CDS             6430..7434
                     /locus_tag="U2K71_001532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315617.1"
                     /translation="MSDNQLLEVQNLHTGFRLKDDYYDAVDDVSFTLDKNEILAIVGE
                     SGCGKSTLATTIMGLLDPNNTKITGEILYNDLNLIDLNETLYNKIRGNDIGMIFQDPL
                     SALNPLMRIEDQIKEGLSYHTKMNAEQRQARALELLEQVGIPNPARVGRQYPHELSGG
                     MRQRVIIAIAIACKPPIVIADEPTTALDVTIQAQILDLLKDLQEETKTGIILITHDLG
                     VVAEMADRVAVMYGGQFVEVAGVKELFDHPKHPYTRSLLNSIPQEGNHDAELHVIEGV
                     VPSLKNMPRKGCRFAPRIPWIPASDHEEEPILHEVAPNHLVRCSCYKHFHFRDEKGEV
                     "
     gene            7435..8379
                     /locus_tag="U2K71_001533"
     CDS             7435..8379
                     /locus_tag="U2K71_001533"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315618.1"
                     /translation="MTELITIKDLKVHYPIRSGFFNRVTDHVYAVDGVDFIIEKGKTY
                     GLVGESGSGKSTTGKAVVGLEKVTAGQIMYEGKDVTKRSNRKKMGYNKDVQMIFQDSM
                     SSLNPKKRVLDIIAEPIRNFERLSDQEEKKKVKGLLDIVGMPEDALYKYPHEFSGGQR
                     QRLGVARAVATNPKLIVADEPVSALDLSVQAQVLNFMKRIQQEYGLSYLFISHDLGVV
                     KHMCDNIAIMYKGRFVEIGTREDIYNDPRHIYTKRLLSAIPQIDVENRESHKENRRRV
                     EQEYIQNQKEYYDATGRVYDLRTITTTHKVALKDGGAS"
     gene            8379..9341
                     /locus_tag="U2K71_001534"
     CDS             8379..9341
                     /locus_tag="U2K71_001534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315619.1"
                     /translation="MWKTILRRVLLMIPQVIILSVLIFLLAQAMPGDPFTGLINPNQD
                     PAVIEQMRQAAGLNDPWYVQYFRWIGNALQGDFGQSFLYKRSVADLIGERIINTLYLS
                     ILTVVLTYLIAVPLGMLAGRYQNSILDKIVVIYNFFSFAVPLFIFGLIMLFVFGYRLG
                     WFPTSGSQTLGLQGGFITQWIDRLKYILLPSITQAFLATAVTIQYLRSEVIDSKSLDF
                     VRTARSKGVPTNKVFSRHIFRNAALPMASQMGYEITSLIAGSVVIEKIFGYPGVGKLF
                     IDSIGQRDYTVITSLVLILGIATLVGTLLSDIIMSIVDPRIRIQ"
     gene            9368..10282
                     /locus_tag="U2K71_001535"
     CDS             9368..10282
                     /locus_tag="U2K71_001535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315620.1"
                     /translation="MSEKNNTAVQESIPPMGIRMIAREFKKDKIAIFSLGLLVVLLLV
                     IFIGAMLTDQDKVMYVSIFDKYAAPGTVSTQGQKFILGADEGGRDVLGQLIIGARNSV
                     TIGFAITILTSIIGVGLGIISGYYGGIIDNILMRIVDFIMILPIMLIIIVFVSIISRY
                     NVWSFIFIMSAFYWVAKARLFRSKTLSEARRDYVSASKTMETNDFKIMFREIMPNLSS
                     LIITNLTMNFAANIGIETTLTFLGFGLPANVPSLGTLIGYASNGEVLVNRQWIWLPAS
                     ILILVLMLSINYVGQAFKRSADARQRLG"
     gene            10308..12089
                     /locus_tag="U2K71_001536"
     CDS             10308..12089
                     /locus_tag="U2K71_001536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM7315621.1"
                     /translation="MKKTIFGFAALMSAVVLAACGGGGTDTSGNNSGGNGNNKEELTF
                     PIATKNNDDAIEDGELEAAVATDSQFKGLFQWEFYQDSYDAAFMAPSHEPLFANDDDF
                     RITDDGAATLDLDEDTKKATIKVKDNVKWSDGEDVTADDVIFPYEIIGNKDYTGIRYD
                     DTFRNIVGMEEYHDGSADTISGIKKIDDKTVEIEYKEVNPSMLQSGGGIWSYAAPKHT
                     LEGVAIKDMESSDQVRKNPVTFGPYYMSNIVAGESVEYLPNEHYWNGTPKLKKITMKS
                     VPMASATEALNSKLYDMIFQMPTDTYDTYKEIAGYTNLGREQTSYTYLGFKLGKWNAD
                     TKSVEYNPDSKMADKSLRQAMGYALDNNAVGERFYAGLRSNATSLIPPVFQSYHDDEM
                     KGFTQDLDKAKKLLDDAGYKDVDDDGLREDKDGNKLTINFASMSGGETAQPLADYYIQ
                     QWKEIGLDVQYTTGRLIEFNSFYDKLENDDPEIDVYQAAWNTGTDPNPSGLWGPNAAF
                     NYTRYESEENTKLINDISSSKAFDEEYQKEAYQKWQQYAFEEAFAIPTLFRNEVLPVN
                     DRVKDWDWSYDVPNPWANVSVTADSRS"
     gene            12437..13123
                     /locus_tag="U2K71_001537"
     CDS             12437..13123
                     /locus_tag="U2K71_001537"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HEM7315622.1"
                     /translation="MDNQLTKELKENYNIVFHDLNLLEQAFTHSSYVNEHRNLQLSDN
                     ERLEFLGDAVLELMVSQYLFRLYPHLPEGKLTKTRAAIVREDSLSKFAKECHFDQYVL
                     LGKGEENSGGRTRPALLCDLFEAFLGALYLDQGFDTTHAFIEKVIFPKVKAGAFSHEM
                     DHKTKLQEVLQKSGDVSIEYRLINEEGPAHERVFWIEVYVDDQLIGTGQGKSKKLAEQ
                     AAAENALAAL"
     gene            13143..16724
                     /gene="smc"
                     /locus_tag="U2K71_001538"
     CDS             13143..16724
                     /gene="smc"
                     /locus_tag="U2K71_001538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HEM7315623.1"
                     /translation="MYLKRIEIAGFKSFADKTVIDFENSVTAVVGPNGSGKSNITEAI
                     RWVLGEQSAKSLRGGKMPDIIFAGSDTRKQLNIAEVTVILDNSDHYLPLEYNEISVTR
                     RYRRTGESEFFINKQSCRLKDIQELFLDSGLGKESFSIISQGKVEAIFSSKPEDRRGI
                     FEEAAGVLKYKQRKKKAEQKLFETEDNLSRVQDIIHELEEQLTPLAAQSEAAKEFLRL
                     KETLTQTDVSLMVAEIKTAKKDWDNKQAQLAKFNLELGKLSESIQEQESILAKQRKEN
                     AQADRLIEKNQQVLLDLSEKLKQTEGQKDVLQERTKHTQKSSQEYQTSLAEAQKKVKH
                     FEKLQESLMKAAAEKETEIQKAEANLIKTQQELEKYQKSTKELLAELRDQYVDLMQEQ
                     AAVGNELKYLERQYIQETAKSKQTLAKQSEVEASVDRLILQKEELTQKQAQLKSSLTE
                     TKEKLEMIQQNGKKFQEALAKEQPKMYQLMNQVQQLRARKKSLQEIQENYFGFYQGVR
                     LVLQHKQQLSGIVGAVAELIDVPADFTLAIEAALGGAAQHVIVENEKDARQAITYLKQ
                     QRGGRATFLPLTTIKPRQLPAHILTQAAAVEGFIGIASEQVSYPDQIQTVVQNLLGTI
                     LLAKDLTSANAIAQTIRYQYRVVSLEGDVMNAGGSMTGGANKRGNQGSLFVQNQELKQ
                     LTSEFEEADKQLQAQEKKVQELQQETARLAEEQEVLRTRGEQLRFEEQEATNQLQNII
                     NELERFEKEKQISTFETRELQQFIEDYEKQTNELKDKQTDLESQRQKIDEEIKSLSQE
                     SDQMEARRAQVQSQKAQEQADLAVLKEQFNHLQIQLRGARVQKAEATERQEAIEKQLA
                     TLTADFSDHEVTEESLEKQINELSAQRETLKAELAKAKEQRDRRQKEIDKLEAVLAER
                     NREQKARLSEQSKLEVQKDRAEMILDNHLTYLQSEYQLTFEKASQDYQETTDIEDSRT
                     KVSSLKEQIEKLGPVNLNSIEQYEQVSERHTFLATQRDDLLAAKNQLFETMDEMDDEV
                     RTRFKEVFEAIRQEFKIVFPNMFGGGKAELVLTEPTDLLKTGIEIEVQPPGKKLQSLS
                     LLSGGERALTAIALLFSIIRVCPVPFCILDEVEAALDEANVKRFGRYLSDFQDDTQFI
                     VVTHRKGTMVAADVLYGVTMQESGVSKIVSVRMEDINEEAKINETKV"
     gene            16733..17542
                     /locus_tag="U2K71_001539"
     CDS             16733..17542
                     /locus_tag="U2K71_001539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM7315624.1"
                     /translation="MIKLVAIDLDGTLLNEQKLISDENKQALAQAKQQGVKIVLCTGR
                     PLAAMAHYLQELGLVDEGDYSITFNGGLVQKNDTGEIMEKKVMEVSDIQRLYKLAQQL
                     DLPLDVLSDNVVLQLPSAPRKQSLYNVLNKLLQFQPADISDITDEFVLNKAVIAYDQE
                     ELDPKIKEIPAEFHEMYEIIKTRSMLLEFMPKGVTKAYGISLLAKDLGLKQEEIMAIG
                     DEENDLPMIQYAGLGVAMENAVPFVKEAADFVTASNEENGVAKVIQKFVLE"
     gene            17553..18551
                     /gene="ftsY"
                     /locus_tag="U2K71_001540"
     CDS             17553..18551
                     /gene="ftsY"
                     /locus_tag="U2K71_001540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HEM7315625.1"
                     /translation="MGFFDKIKRAFTGEEKTEEVEVQEKYDKGLEKTRKTFGERMNEL
                     FANFRTVDEDFFEELEETLIGADVGFETAIRITEALRQEVKLRNAKKSAEVQNAIIEK
                     LVDLYEAEGINEVNELNIQEDGLTVILFVGVNGVGKTTSIGKLAHQFKNEGKKVLMAA
                     ADTFRAGAIDQLVVWGERAGVEVVRGNAGGDPAAVVFDALERAKTEQADVLLVDTAGR
                     LQNKVNLMNELEKIKRVIQRELPEAPQETLLVLDATTGQNAMVQAKQFKETTDVTGLV
                     LTKLDGTAKGGIVLAIRNELHLPVKLVGLGEGIDDLEPFDPNDFVVGLFKGLLKEE"
     gene            18738..21005
                     /gene="gshAB"
                     /locus_tag="U2K71_001541"
     CDS             18738..21005
                     /gene="gshAB"
                     /locus_tag="U2K71_001541"
                     /EC_number="6.3.2.2"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional glutamate--cysteine ligase
                     GshA/glutathione synthetase GshB"
                     /protein_id="HEM7315626.1"
                     /translation="MMNFKQLLLHVNARPFIDQARFGIEREGQRVDLAGNLAKTDHPA
                     IFGDRSYHPYIQTDFSETQTEMITPVTDSIPELFQYLAAVYDVTARSIPKEEMIWPLS
                     MPPALPEKDEEIIIAKLKNFEDVLYRRYLAKKYGKRKQMVSGIHFNFEFGDELLRTLF
                     SHQEEFQDFSEFKTELYLKTARNFMRYRWMITYLFGASPMSEKNYFLDESHPQEPVRS
                     IRNSALGYTNHPNVKVSYASMKQYLADIERMIEEGKLSEEKEFYTPLRFRGGKKVADL
                     ATTGVRYIELRNIDLNPYARLGINPEQVRFLQLFLMYMLWTEEKEDCDQWVAEGTTRN
                     NKVALEQPSDQTEFHQEGREILEGMKQMLVELDWLDSLYLVEEALTQMDHPEQTLAAK
                     LYQEAQLSSQQEVAVALGHQYYKESHERPYQLAGFREMELSTQIFMFDAIQKGVQVKV
                     LDESDQFLRLQFQDHVEYVKNANMTSKDSYIVPLIMENKTVTKKVLKEAGFRVPGGAE
                     FSSMEEAVKAYPRFAEQAFVIKPKSTNYGLGITIFKEGASLEDYQAGLAIAFREDSSV
                     LVEEFMPGTEYRFFVIDGEVQAIMLRVPANVIGDSIRTVKELVEEKNSDPLRGTNHRA
                     PLELIQLGELEQLMLKEQGLTIESVPQANQIVYLRENSNISTGGDSIDMTDEFSEAYK
                     KIAVSAVEALGAKISGIDLIIPDKEIDPTTDKKAYGIIEANFNPAMHMHVYPFAGKGR
                     RLTMNVLKLLYPEVF"
     gene            complement(21094..21546)
                     /locus_tag="U2K71_001542"
     CDS             complement(21094..21546)
                     /locus_tag="U2K71_001542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YueI family protein"
                     /protein_id="HEM7315627.1"
                     /translation="MAQDDVKSHLDKGLYGTPLVNPEEQHKYLGTFRERCYLSMTVAE
                     MIQPKNKENFLKELKKHPDSSILLNGALPLSVQNQYIQLATKNNSRFTVVNDYVTANP
                     EEFGLLLTAKEAVNEPVIDIEKKYPEENSSPAEKEKPKKKKSFWDRLF"
     gene            21696..22646
                     /locus_tag="U2K71_001543"
     CDS             21696..22646
                     /locus_tag="U2K71_001543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEM7315628.1"
                     /translation="MRYGRLVALLVILSSISLFIGVSNVQLSELLQLDGQSLQILLVT
                     RLPRTICLILVGATSSICGLIMQHLTQNKFVSPTTAGTMDSARLGILVAMIFLPGASL
                     IVRSLTAFCFAFAGTLLFLSLTRLFLQKNQLMLPLIGVMLGNIIGSVATFFAYQFQLV
                     QNMTSWLQGNFATVIRGNYELLYAVVPLLLLVWLFAYRFTIIGLGESFAVNLGIDYQK
                     MQFLGIGLVSLASAVMLIMVGSIPFIGVIIPNLVSRIYGDQVHKTIGVTALAGSLFLV
                     FCDLVARVLIFPYEIPVSVIVGIIGGAIFLYLLIRGRRYA"
     gene            22639..23598
                     /locus_tag="U2K71_001544"
     CDS             22639..23598
                     /locus_tag="U2K71_001544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron chelate uptake ABC transporter family
                     permease subunit"
                     /protein_id="HEM7315629.1"
                     /translation="MLKKKPSIFIGMLVLVLFVTGMYLTIHTYGNWHFACALRSKKIF
                     AFILVSLAVSSATISFQTITHNQFLTPSVLGLESLYVLVQTVLFFFIGGVTMLQQTGT
                     VFFLLNVGLMVGLSLLLFFFLLKKMRANLFLLLMSGMILGTLFGSVSTFLQVIMDPNE
                     YDLLQGRLFASFGNIQSQYLLPASLIIIGCWYLLFRYRHVLDVFHLGNEQAVNLGIDT
                     DRFEFLILCCVSLLTGTSTALVGPVTFLGFIVANISYRVFSTYRHGQLFIGSFLVAML
                     FLTGGQLAVEQIFGWNTTVSVVVEFVGGIYFVTKLIRERKSIL"
     gene            23595..24350
                     /locus_tag="U2K71_001545"
     CDS             23595..24350
                     /locus_tag="U2K71_001545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7315630.1"
                     /translation="MIDLQKVNKLYHGKSVVKDVQLTIPEKKLTAFIGPNGAGKSTVL
                     SMISRLIPKDTGEIYLDHNEVKAWKSNELAKKLSILRQSNEINVQITVKELVEFGRFP
                     YTKGKLTTKDEEIVQESLEHLGLEEIADQSIDTLSGGQLQRAYIAMVLAQDTDYILLD
                     EPLNNLDMNFAVQIMQILKNLVQEIGKTIVIVLHDINFAASYADEIVAMKDGQLFTQG
                     TTQEMIKKDVLDELYEMDIRICEIEGRRFCLYF"
     gene            24373..25326
                     /locus_tag="U2K71_001546"
     CDS             24373..25326
                     /locus_tag="U2K71_001546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="siderophore ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM7315631.1"
                     /translation="MKKLVVGILILASFGLAACGNSNNTSQADTKKSSTQTETTLTIT
                     DSNGDQIEVPNNPEKVVVFDNGSLDTMDALGVGDKVVGAATSSLPEYLSSYKKVESAG
                     GIKEPDLEKINQLQPDLIIISGRQRDFQKDLSQIAPTIFLSLDAKNPWESFQQNVTAL
                     GEIFGKQEEAKTQLEELSSAIDQTKKKAEATDKKALVTLVNEGQLSAYGSGSRFGFIH
                     DLFGFEQADDQIEASTHGQSVSYEYVLEKNPDILFVVDRTKAIGGDDSKDDISANELV
                     AQTNAGKNQQIISLEPDVWYLSGGGLESMKLMIEDVNQAFK"
     gene            complement(25970..26266)
                     /locus_tag="U2K71_001547"
     CDS             complement(25970..26266)
                     /locus_tag="U2K71_001547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanyl-tRNA synthetase subunit alpha"
                     /protein_id="HEM7315632.1"
                     /translation="MKEYIKNIYFIEETQNIEGSYIEVKTLFVNEDKTKALDIYKKLA
                     SKKTNSFGLILSEYKIKAEESYFYQLLKRWSKLPADFYRKMQIINYQPLAETHA"
     gene            26621..26977
                     /locus_tag="U2K71_001548"
     CDS             26621..26977
                     /locus_tag="U2K71_001548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEM7315633.1"
                     /translation="MKLEVTKEAQEVLKNKLEADDQLLLDIENGDGPFAESQVSCQLD
                     TAFRLIIVKKDTQTDLSLYSVVADTPVGPIKIKDSAILYMDDPSTLALEPTYNSLQLK
                     GPSGLRKGNLQVVRKD"
     gene            26987..27886
                     /locus_tag="U2K71_001549"
     CDS             26987..27886
                     /locus_tag="U2K71_001549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphohydrolase"
                     /protein_id="HEM7315634.1"
                     /translation="MGKLAIISDLHVDINKFGESELMQLWQVLQKHKITRLHLAGDTA
                     NKVDRCLAVVEFFVNKGLSTTFHFGNHELADVPNEQMMDHFPDPHFLNERYIALNEQT
                     VLLGMNGWYDYQFSELKDYEAIVRMKRLYWYDRFIQRGKTDLEVNAEMLAAAKNLLDQ
                     LEAKKLEVILSTHFVPKRAFIMYQSAPYERWNKLNAFLGSESFGELLDRYSNIKQVVF
                     GHTHRRFEDKSIGHTIYSCRPFGYYYEWQLTRDFVLKEQLLDSYDPMKLRVLLRAHHP
                     AFQAYQTQHLQEEFEQAMTIVPY"
     gene            28403..29134
                     /locus_tag="U2K71_001550"
     CDS             28403..29134
                     /locus_tag="U2K71_001550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WxL domain-containing protein"
                     /protein_id="HEM7315635.1"
                     /translation="MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEA
                     GDEGVTPPVDPENPDPNKPVDPSDPPSPGTGGALSIDYGSKFKFGTQKISTADKTYYA
                     AADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAV
                     SLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSV
                     PGKSVKLAQQYSTKLVWTLEDTPNN"
     gene            29279..31306
                     /locus_tag="U2K71_001551"
     CDS             29279..31306
                     /locus_tag="U2K71_001551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucBP domain-containing protein"
                     /protein_id="HEM7315636.1"
                     /translation="MKKRRIVLILSSLLVTNIFPIIGYSLELNQLEHGNRRDEESKLT
                     STFDSLIESNQTQESSEDLSISSSGETTDNVEAEGSDKQGLEDAPISAVELPDDPNII
                     SIDKIFQTPIGTSTSILENGKLLQLNPAERSQRGAIWSNKKISLLSDFTFKSYLYLGN
                     QRDRAGDGMTFTLTGDPRMASNPSQVIGSPGMGIGSYSTRSGQPYIANALSIEFDTYK
                     NSGSSDRMDREITADNRGHGHVAFVTPKPNNNNYSGEHTGVTVAPTFLSNGTWRLLII
                     KWDAEQRSLTYDLEGVGRSSRIIPDLIRQFGSTEVNWGFTSSTGTFFQENAVAVTRLP
                     SNVTSTADVKVNDSDYGAIAEAGGNDKITIRNTLKVEGDFLEYKKMQTSIDLPQELTI
                     PEHILINGNQIDAKDILITGNRVTLNLTDYFIAGDTLIIELETTLQEVIPEKVLTMHF
                     EYLENGNLIEQSNEINLKIAKLKEIDIHVYYKDKDSQIDIAEMKTITGKIGEKYKETP
                     IDIEGYVFDSDSANTEGIFSENTEDIYFYYRKGKLYLSTAPQYVNFGKHKISSRPLSE
                     FGQFSGGLKIIDERAFGSWKLQLKQEKVLTNGDFELPEALSFVTSENTTVIGTSAVTI
                     FESDQKGESDLSSLLDSTAHRGIRINIPVENQRIGTFEGKLSWILADVPGN"
     gene            31414..32511
                     /locus_tag="U2K71_001552"
     CDS             31414..32511
                     /locus_tag="U2K71_001552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF916 and DUF3324 domain-containing protein"
                     /protein_id="HEM7315637.1"
                     /translation="MIKGAIFLRPKQQLFITLLVILGALFNNISVSASAFNFSVTPIT
                     SENQIDKRKTYFDLQLDPDQEVEVKAELRNDTEKEVKIDISVNSATTNSNVMVEYGKN
                     EIEKDMSLIFDLVDYVSYPQIVTLKPKSVQTVAFHVRMPNERFDGVLAGGITFQEIQT
                     EKDQTETKDQSLSIENEYTYIIALLMQQTLNEVAPNLTLHEVKPDQINARNVILANVQ
                     NDQKTYINQVVIETKITKKGHSEVLYQEEKEGLQIAPNTNFSFPTALNGQPLTPGEYH
                     LTMTVLGNENENGKFSRKKGNTTINYTNQWVFEKDFTIDGKVAKELNTTDVTIKKDNN
                     WIYILVASLFLLFILLCILLFVLRKKEEKEK"
     gene            32818..>33263
                     /locus_tag="U2K71_001553"
     CDS             32818..>33263
                     /locus_tag="U2K71_001553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315638.1"
                     /translation="MNYEFFLSKEDHRSYSLLKYLEASSNLSESISNVQEELSLSTFL
                     LKKTIDKLKHDLQKFDLEKNLRVTVSELDICLEINGKCSSKALLSRYITDSLSMKMVL
                     AFFQEKYISVENFAKQHHVSYSVAYKVLQRLKRNLKKYQIFFEKRK"
BASE COUNT        11139 a         5761 c         7123 g         9240 t
ORIGIN      
        1 aaaaaccgtt cactatctga acggtccaag tatcattcaa ttaaacgcgt tcaacttttc
       61 ctgatttcaa agcacgagtt gacacccaaa cttttttagg ttttccgtcg accataacgc
      121 gaactttttg taagttaggt ttaacagtac gttttgttga gttcatagcg tgtgaacggt
      181 tgtttccact ttttgtcttg cgacctgtaa agtaacaaac ttttgccatg gtaatttcct
      241 cctttgcctt gatttggagc aatagaataa ttcagctcat accagactaa catatcatat
      301 agaatggaaa tttgcaagta cattttcata gatacaattg acaattgaga agaataattg
      361 taaattaaac aaggttatac ctgaaatttt gcaaggtttt tgagtacttt gggtaactgc
      421 cttttatttc aagttgcgct atgataaaat atgatagaag aaaaatggga gttttttacc
      481 cgttaaggag gctcattcat ggctgtaaaa attcaaacgt cagctggaac cattgaaatt
      541 accaatgatg taattgcaac agttgttggt ggcgccgcga cagatgtgtt cggtgttgta
      601 ggaatggcca gcaaaaatca aattaaagat aatattactg agattttgcg taaagaaaat
      661 tactcaagag gagtagttgt ccgccaagaa gataatggta ttgctgtaga tgtttatacg
      721 attatcagct atggtacgaa aatctctgaa gtttcccgca acgtccaaga aaaagtgaaa
      781 tacaatctag aggcattatt aggcgtaact gccaattcag ttaatgtctt tgtacaaggt
      841 gttcgtgtac tgccagacta ggcaggagac tggctggtaa ctcagcagtc gatctgaagg
      901 aggattatct aggtgagtgt aacagaaata agtgcaagcc agttccagga aatggtccag
      961 gcaggtgcga atcgtctgca agtaaatgca gaatacgtca attcattaaa tgtattccct
     1021 gtgccagatg gcgatacggg aacaaatatg aatctatcta tgactagtgg agctaaggca
     1081 gtagtagatt caacctcaga aaaagtagga gaattagcag caatgctttc aaaaggattg
     1141 ctgatgggtg cgcgcggaaa ttccggcgtt attttgtctc aattgtttcg tggattttct
     1201 aagcaaattc cagatgttgt ctcattaaat gcttccgatc ttgctgcagc cttcactcat
     1261 ggagttgaaa ctgcgtataa agcagtaatg aaaccagtag aaggaacgat tttgacagta
     1321 gctagagaag cagcgaaagc tggagaaaag aaagcaaaaa gtactgatga tgtcgttgaa
     1381 gtaatgacag ctgttgtaaa aggggcaaag cgtgctttag ctaaaactcc agacttgctt
     1441 ccagtcctaa aagaagtcgg tgtcgtagac agtggagggc aaggactgct gtttgtttat
     1501 gagggctttt taagtgcatt gaatggagaa ttccaagcgg atgaaacata tgaaccttct
     1561 ccagcagaaa tggatgatat ggtcaatgca gaacatcatc gcagtgtcca aggtcaaatg
     1621 gcgacagaag atatccattt tggttactgt acagaaatca tggtaaaaat cggagaagga
     1681 cctacagtcg atagtacatt cgattacgaa gaattccgta actatttaga tggtatagga
     1741 gattctttac ttgtggtcaa tgatgacgag atcatcaaag tccacgtgca tacagaaaac
     1801 ccaggagaag tcttgaatta tggacaaaaa ttcggttcat tagtcaaaat caaagtcgac
     1861 aacatgcgct tgcagcatga gacgattttg gaacacgatg aagaagtgaa cgagtttgcg
     1921 aaaaaagaaa caccacgtat cccgtatggt atcgtagcga tcgctgctgg taaaggcgtc
     1981 caagagctgt ttgaaagttt aggcgctaat tatgttatca gtggtgggca aacaatgaat
     2041 ccaagtacgg aagacattgt taaagcgatt caagaagtaa acgcagataa agtgattgtc
     2101 ttgccaaaca ataagaacat cttcatggca gctgatcaag cagcagaagt agcagatatc
     2161 ccagtagcag ttgttccttc aagaacgatt tcccaaggat tgacagctat gctttcattc
     2221 aatgatcaag cttctttaga agaaaacaaa gaaacgatga cagaaatgtt gtctagcgtt
     2281 gtgagcggtc aagtcactca cgcgatcaga gatacctcaa ttgacggtgt agagatcaaa
     2341 gaaggagact ttttgggcat gatcgatgga aaaatcctga tctctaaccc agatatcaca
     2401 gcaacagcgt taagtacact agaacaaatg atcaatgagg acacagagat cgtaacgatc
     2461 ttggcaggtg atgaaggaac agttgaacaa gcaaatgaat ttgctgagaa actaacagaa
     2521 aaacatcctg atttggaaat cgagatccac caaggagatc agcctgtcta tccttatcta
     2581 ttttctgctg aataatttct gtgtcagaat atctttctaa cacagtattc attcataaaa
     2641 ggccttcggt tagtgatcag gtatgatcgc tgaccaagtc ctttttttgt taagggaggg
     2701 aggaaaaatt tatgcgttct ttagatgatc caatcactat gctggcagga gtcggagaaa
     2761 aaagagcagc tagtcttgcc agcttgggca tccaaacgat tgaagactta ctgacttatt
     2821 atcctttccg ctacgaagat attcaggaaa gaaatctaaa cgagatccaa gatcaagaaa
     2881 aggttacttt gaaaggaatc gtagtctcgc ctcctgtgat gaatcgattt ggttataaaa
     2941 aaagccgatt gcaatttcgt atgatgcaag aacatgatgt gttcaacgtt tcttttttta
     3001 atcaacctta tctaaaagaa aaagtcattt tatcggagga aatcgccgta tacggtaaat
     3061 gggatgcaaa gcgtaaagca ttgaacggga tgaagatttt aggttctcaa acaatggatg
     3121 acttttcgcc gatatatcac gtcaacaaat caattcgtca aacgacatta gttgatctaa
     3181 tcaaaaaagg gtttcaagaa tacggcacat tgattgaaga aaatctgcca aacaaccttg
     3241 tggagaaata tcgactgctt gaccgagctt ctgctgtaag aagtatgcat tttccaaaag
     3301 accacgaaga aaaccatcaa gccaaacgac gtgtcgtttt tgaagagttc tttttatttc
     3361 aaatgcgtct tcaaggattg aaaaaagcag aaaaagcaga aacgaatggt ttggagatcc
     3421 tttatgatgt ccaaaaattg aaagaattta ctcaaaagct accttttgaa ctgacaaatg
     3481 cacaaaaacg tgtaacgaat gaaatctgtc gggatcttcg cagtccgcag catatgcagc
     3541 gacttttgca gggagatgta ggtagcggaa aaacagtggt tgccgcgatt gcgttatacg
     3601 ctaccgttac agcaggtttc caaggtgcgt tgatggtgcc tacagaaatc ttggcacaac
     3661 aacatatgga aagcttgcaa cagctgtttg atcccaatga agtaaccatc gcattgctta
     3721 cggggtcaac gaaagcaaaa gaacgtcgtg agctccttga aaaactagag caaggggaaa
     3781 ttaacatcgt gatcggcaca catgcattga tccaagatgg agtcgaattt cagaatttag
     3841 gtctagttat tacggatgag cagcaccgtt ttggcgtgaa ccagcggaaa gtgcttcgtg
     3901 aaaaaggctg gcgaccagat gtcttattta tgacagcgac tccgattcct agaacattag
     3961 ctattacagc atatggtgag atggatgtat ctatcattga tgaacttcca gctggacgta
     4021 tcccgatcga aacacgctgg gtaagaacac ctcaattgga cagtgtactg gattggacga
     4081 aaaaagaact ggcaagagga catcaaatgt atgtcatctg tccgctgatt gaagaatcag
     4141 aagcattaga tgtaaaaaat gcagtggaaa tctatgaaaa acttcgtgaa ctatttgcac
     4201 caaaatatga agttggcttg ttacatggta aaatgaagaa tcaggaaaaa gaagtaatca
     4261 tggaagcctt caaagacaac cagatgcaga tattagtttc gactactgta attgaagtag
     4321 gggtaaacgt gccgaacgcg accgtcatgc tgatcatgga tgcggatcga tttggattgg
     4381 cacagctgca tcaacttcgt ggacgtgttg gacgcggaag tgatgcttcc tactgtatct
     4441 tagttgctaa tcctaaaaat gaactaggtg tggaacggat gaaaatcatg acagagacca
     4501 ccaatggttt tgtactaagc gaaaaagatc tggaattacg tggtcctggt gaagtatttg
     4561 gcttccgtca atctggtctg cctcaatttg cgattgctga cttggtgacg gatggaaatg
     4621 ttttggaggt agccagagaa gaggctgcag ccctttggca gataaaggat tggcagatgc
     4681 ttcctgagta tcagccttta gcgcaaagcc tccaaataga aaatcaggat aatcgttatt
     4741 tcgattagca gactgtgcta tactttatac tgatgacgga aaaaggagtg gaaacaatga
     4801 aaattgccgt agatgcaatg ggcggagatc acgcacctca agcaattgta gaaggcgtgg
     4861 ctttagcgca gcaagatttt ccagaagtag aattcttact ttacggaaaa gaagcagaaa
     4921 tcaagaaata cttaacaaat gaaaaaaaca tcacgattat ccatacagac gaaaaaatca
     4981 atagtgatga tgagccagta aaagcaattc gacgaaaaaa gactgcatca atggtccttg
     5041 ctgcacaagc agtgaaaaac ggtgaagcag atgctgtatt ttcagcaggg aatacaggcg
     5101 cgctactagc agcagggttg ttcatcgtag gacgtatcaa aaatatcgaa cgccctggct
     5161 tgatgtctac cttgccagta gtagggaaag aaggtgccgg tttcgatatg ctggatcttg
     5221 gcgctaacgc agaaaacaaa ccagaacatt tgttgcaata cggtatttta ggttcttttt
     5281 atgcacgtaa agtccgaggt atcggacgtc ctcgtgtcgc actcttaaac aacggaacag
     5341 aagcaacaaa aggtagtgaa gtgacgaaaa aagcctatga actccttcag aatgaaacaa
     5401 gcttgaattt tattggaaac gtagaagctc gtgagctact caatggtgtg gctgatgtag
     5461 tggtcacaga tggatttaca ggcaacgctg ttttgaaatc tattgaagga acagcaatga
     5521 atatgatgaa tctattaaaa tctgctattc taaatgaagg gatcaaagga aaaatgggcg
     5581 ctatgctttt aaaagacggc ttacgttctt tgaaagcaga aatggattat tccaaacatg
     5641 gcggtgccgt tttgtttggc ttgaaagcac cagtcattaa aactcatgga gcaacaggcc
     5701 ccgatgctgt tcgctatacg atccgccaaa tccacacaat gcttgagaca gatgtagttg
     5761 gacaattagt cgaacaattt gaaaaaagcg aagaatagta tttggatatc tgaagcaatc
     5821 cgttccattg aaaaaaaggt ggacattcct gaaaaatgcc agtaaaatta gccatggtgt
     5881 aggtaattga caagtgtatg attcttggag gtgaaaaagt tgactcgcga agaagtattt
     5941 aacaaagtag cgaaaattat ttcaaatcat tttgagatag ataccgataa agtgacggat
     6001 gaattgaaca tcaaagatga tttaaaagct gattcaatta gtattatgga attcgtttta
     6061 gagcttgaag atgaattcgg tacggaaatt tcagatgaag acgctgaaca aattgaaaca
     6121 gtcggtggcg cagtagatta tatcagcagc catcttaaat aatttcaaat aaaagcaaag
     6181 aaggataaaa agctgcatac agctttttat ccttcttttt ttaatctttt tgacacgtaa
     6241 aagtcatttt tctgataatt ctggcgataa acagaaatta tgttgcaaag taaagcgatt
     6301 ttctatataa ttattaataa ttcattacac aaagataaga ataacctttg ttataaaacg
     6361 ctttattctg tgtaatgtcc ttttttatta agaaaaactt ccatttagga agcagagagg
     6421 ggtattcgat tgtccgataa ccaattatta gaagttcaga acctacatac aggattccga
     6481 ctcaaagatg actattacga tgcagtagat gacgtttcat ttactttaga caaaaacgag
     6541 attttagcaa tcgttgggga atctgggtgc gggaaaagta ccttggctac tacgattatg
     6601 ggattacttg atcctaataa cacaaaaatt acaggtgaga ttttatataa cgatctgaat
     6661 ttaatcgacc tgaacgaaac gctctataat aaaatccgcg gaaatgatat cggaatgatt
     6721 ttccaagatc cgttgtctgc attaaatcct ctcatgagaa tcgaagacca gatcaaagaa
     6781 ggactttcct atcacacaaa gatgaatgca gaacagcgtc aagctcgtgc actagaattg
     6841 ctagaacaag tagggattcc taatcctgcg agagttggac gccaatatcc ccatgaacta
     6901 tcaggcggta tgagacaacg tgtcatcatc gcaatcgcaa tcgcctgcaa acctccaatc
     6961 gtcattgcag atgaaccgac gacagcccta gatgtaacca tccaagcaca gattcttgat
     7021 ttactgaaag accttcagga agaaacaaaa acagggatca tcttgatcac tcatgactta
     7081 ggagtcgtag cagaaatggc tgatcgagtg gcagtgatgt atggcggaca gttcgttgaa
     7141 gtagcaggtg taaaagaact gtttgatcat cctaagcatc cttatacacg ctctttactg
     7201 aactctatac cacaagaagg aaatcatgat gcagaactcc atgtgatcga aggtgtcgtt
     7261 ccttctctga aaaacatgcc tcgtaaaggt tgtcggtttg caccacgtat tccatggata
     7321 ccagcaagcg atcatgaaga agagcctatc cttcatgaag tcgcaccgaa ccacttggta
     7381 cgttgctcct gctataagca tttccacttt agagacgaaa aaggagaggt gtagatgaca
     7441 gaattaatca caatcaaaga cctgaaagtt cattacccta ttcgcagtgg attcttcaat
     7501 cgagtaacgg atcatgtata tgctgtagat ggtgtggatt ttattatcga aaaaggaaag
     7561 acatatggtt tagtgggaga gtctggctct ggaaaatcta ctacgggaaa agccgttgtg
     7621 ggattagaaa aggtaacagc cggacaaatc atgtatgaag gaaaagatgt aacgaagcga
     7681 agcaaccgca aaaaaatggg ttataacaag gatgtgcaga tgatcttcca agattcaatg
     7741 tccagtttga atcctaaaaa gcgagtgttg gatatcattg ctgaacccat ccgaaatttt
     7801 gaacggttaa gcgatcaaga agaaaagaaa aaggtaaaag gactgctaga tatcgttggg
     7861 atgccggaag atgcgctata taaatatcct catgaatttt caggcggaca acgtcaacgt
     7921 ctaggagtag cccgtgctgt tgctaccaat ccaaaactga tcgtagccga tgaacctgta
     7981 tctgctctgg atctatctgt tcaagcgcaa gtattgaatt tcatgaaacg aatccagcaa
     8041 gaatacggcc tcagctattt attcatttca catgatttag gtgtagtaaa acacatgtgc
     8101 gacaatatcg caattatgta caaaggtcga tttgtagaaa tcggtacaag agaagacatt
     8161 tataatgacc cgcgacatat ctatacgaaa cgtctgcttt cagctattcc gcaaatcgat
     8221 gtagaaaata gggaaagcca caaagaaaat cgacgaagag tagaacaaga gtatatccaa
     8281 aaccaaaaag aatactatga tgcgactggt cgagtctacg atttgcgtac gatcacaact
     8341 acacacaaag tggcgttgaa agatggaggt gcaagctaat gtggaaaacg attctgcgac
     8401 gtgttttatt gatgatcccg caagttatta ttttaagtgt tctgatcttc ttattagccc
     8461 aagcgatgcc gggggatcca tttaccggtt tgatcaatcc aaatcaggat ccggcagtga
     8521 tcgaacagat gcgacaagca gctggcttga atgacccatg gtatgtccag tacttccgtt
     8581 ggatcggaaa tgctttgcaa ggcgattttg gtcaaagttt cttatacaaa cgctctgttg
     8641 ctgacttgat cggcgaacgg atcatcaaca ctttatatct atcgatcttg acagttgtcc
     8701 ttacttattt gattgctgtg cctctgggga tgcttgctgg ccggtatcaa aattcaatct
     8761 tggataagat tgtcgtaatc tacaacttct ttagttttgc tgttccttta tttatcttcg
     8821 gcttgatcat gctatttgtt tttggttatc gtttaggctg gttcccaaca agtggatcac
     8881 aaacactagg tttgcaaggt ggatttatca ctcaatggat agaccgtttg aaatatattc
     8941 tgttaccatc aattacccaa gcatttttgg cgactgccgt cacaatccaa tatctgcgaa
     9001 gtgaagtaat cgattcgaaa tcgctcgatt ttgtcagaac agcccgttcc aaaggggtac
     9061 cgacgaataa agtattcagt cgacatattt tccgaaatgc agcgttgccg atggcttctc
     9121 aaatggggta tgaaatcact tcgttgattg ctggttctgt agttatcgaa aagatttttg
     9181 gttatccggg agtcggaaaa ctatttattg attccatcgg ccaacgggac tataccgtga
     9241 tcacatcctt agtattgatt ttaggaatcg cgacgctagt ggggacatta ctttcagata
     9301 tcatcatgag tatcgttgac ccgcgtattc ggattcaata aaaccaatca gaaaggagaa
     9361 tggatcgatg agtgaaaaaa acaatacagc agttcaagaa agtattcctc ctatggggat
     9421 acgaatgatt gctcgagaat ttaaaaaaga taaaattgct attttttcat taggtttgct
     9481 agtggtttta ttattagtga tctttatcgg tgcgatgctg accgatcaag acaaagtgat
     9541 gtatgttagt atcttcgaca aatacgctgc accaggaaca gtttcgacac aaggacaaaa
     9601 gttcattctg ggagcagatg aaggcggacg tgatgtatta ggacaattga tcataggtgc
     9661 tagaaactct gttacgatcg gatttgcgat cacgatcctt acttcgatca tcggggttgg
     9721 attagggatc atctctggct actacggagg gatcattgac aacatcttga tgcggatcgt
     9781 tgattttatc atgattctac cgatcatgct gatcattatc gtcttcgtat cgattatttc
     9841 cagatacaat gtctggtcct ttatattcat catgagtgca ttttattggg tagctaaagc
     9901 ccgattattc cgaagcaaga cattatctga agcaagaaga gattatgtca gtgcctcaaa
     9961 aacaatggaa accaatgatt ttaaaatcat gttccgcgaa atcatgccga atctaagttc
    10021 attaatcatt actaatttga cgatgaactt tgcggcaaac atcggtatcg agacaacttt
    10081 gactttctta ggtttcggct tgccggcaaa tgtacctagt ttaggtacat tgatcggtta
    10141 tgcaagtaac ggagaagtct tagtgaatcg gcaatggatc tggttacccg catcaatcct
    10201 tattttagtt ttgatgttga gtatcaatta tgttggacaa gcattcaagc gttctgcaga
    10261 tgcgcgccaa cggttaggct agtatagaag cgaaggggga aataggaatg aagaaaacga
    10321 tttttggatt tgcagcgctg atgtctgctg tcgttcttgc agcatgcggg ggcggaggta
    10381 cagatacctc tggaaacaac tctggaggca acggcaataa caaagaggaa ttaacttttc
    10441 caattgcaac aaaaaataat gatgatgcaa tcgaagacgg cgaattagaa gctgcggtcg
    10501 caacggacag tcagtttaaa ggattgttcc agtgggagtt ttaccaagat tcatatgatg
    10561 cagcttttat ggctccttct catgaaccgc tatttgcaaa cgatgacgat ttcagaatca
    10621 cagacgacgg agctgcaacg cttgatctag atgaagatac gaaaaaagcg acaatcaaag
    10681 taaaagacaa cgtcaaatgg tcagatggtg aagatgtaac ggcggacgat gtgattttcc
    10741 cttacgaaat cattgggaat aaagattaca ctggtatccg ttatgacgac actttccgta
    10801 acatcgtagg tatggaagaa tatcatgatg gaagtgccga cacaatctcc ggaatcaaaa
    10861 aaatagacga caaaacagtt gaaatcgaat ataaagaagt aaacccaagc atgcttcaat
    10921 cgggtggtgg tatctggtca tatgcagcac caaaacatac gttagaaggt gtagcaatca
    10981 aagatatgga atcaagcgat caagtacgta aaaatccagt gacttttggc ccttattaca
    11041 tgagtaatat cgttgcagga gaatcagtag aatacttgcc aaacgagcac tactggaatg
    11101 gtacgcctaa attgaaaaag atcacgatga aatctgtacc gatggcaagt gcaactgaag
    11161 cattgaactc taaattatat gatatgattt tccaaatgcc aacagatact tacgatacct
    11221 ataaagaaat tgctggctat acaaacttag gccgtgaaca aacttcttat acttatctag
    11281 gattcaagtt aggtaaatgg aatgcagata caaagagtgt ggaatacaat ccagattcaa
    11341 aaatggctga taaatcatta cgtcaagcaa tgggctatgc attagataat aatgcagtag
    11401 gtgaacgttt ctatgcaggt ttgagaagca atgcgacttc attgattcct ccagtattcc
    11461 agtcatacca tgatgatgag atgaaaggat tcacacaaga tctagacaaa gcgaaaaaac
    11521 tattagatga tgctggctac aaagatgtgg atgacgatgg tttaagagaa gacaaagatg
    11581 gaaataaact aacaatcaac tttgcttcga tgtccggtgg ggaaacagcg caaccattgg
    11641 cagattacta tatccaacaa tggaaagaaa ttggtttgga tgtccaatac acaacaggtc
    11701 gtctgatcga attcaattct ttctatgata aattagaaaa cgatgatcct gaaattgatg
    11761 tttaccaagc tgcttggaat acaggaactg atccaaatcc aagtggacta tggggaccaa
    11821 atgctgcgtt caactacact cgttatgaat cagaagaaaa tacaaaattg atcaacgata
    11881 tcagttcaag caaagcattt gatgaagagt accaaaaaga agcatatcaa aaatggcaac
    11941 aatatgcatt tgaagaagca tttgcgattc caacgttgtt ccgtaatgaa gtattgccag
    12001 tcaacgaccg tgtcaaagac tgggattggt cttatgatgt accaaaccca tgggcaaatg
    12061 tttctgttac agcagattca cgttcataat cactaaaaat gaccgaaaaa gggcgatgtc
    12121 ctttttcggt tttttgatat ttttctgttt atttctctaa tattcaggtc aaggcgctga
    12181 tttcatgact tctccaataa acggtgttca aagtccagtt tctcggtatt aagtcaaatt
    12241 tatgcaaaaa tatggaaagc cattttcaca aaattttttc ttcatactcg aaactaaacg
    12301 aatttttcac aacttctcgt caatcactag gattattaaa gctttactta ccttttgacc
    12361 gaaccatttc aaatttgaca aaaaaccagt ataataagga agtatcgaaa aaatatccat
    12421 aaaaggaatg tggaaaatgg acaaccagct gacaaaagaa ctaaaagaaa actataacat
    12481 cgtttttcac gatctaaatt tgcttgaaca agcttttacc cattcatcct atgtgaatga
    12541 gcatcgcaat ctgcaattat cggataatga gcgacttgaa tttttaggag atgccgtttt
    12601 agaactgatg gtttctcagt atttattccg tttatatcct catttgccag aaggaaaatt
    12661 gacaaaaaca cgagctgcga tcgttcgcga agacagcttg tcgaaatttg ccaaagaatg
    12721 tcactttgat caatatgttt tgttgggcaa gggagaagag aattcaggag gacgtacgcg
    12781 accagcattg ctttgcgacc tattcgaagc ttttttaggc gcgttatatc ttgaccaggg
    12841 attcgacacg acccatgcat tcatcgaaaa agtgattttt cctaaagtca aagcaggtgc
    12901 tttttcacat gagatggatc ataaaacaaa actacaagaa gtcttgcaaa aatcaggtga
    12961 tgtatcaatc gaataccgat tgatcaatga agaaggtcca gctcatgaac gagtattttg
    13021 gatcgaagtt tatgtagatg atcaattgat tggcactgga caagggaaat cgaaaaaatt
    13081 agcagaacaa gctgcggcgg aaaatgcatt ggcagcactg taaaagtaag tgagggaaag
    13141 ctttgtattt gaaacgaatt gaaatagccg gttttaaatc atttgcagac aagacagtca
    13201 ttgattttga aaatagcgtc acagcagtgg ttggaccaaa tggcagcgga aaaagcaata
    13261 ttacagaggc tatccgctgg gtattaggag aacaatctgc taaaagtctg cgaggcggaa
    13321 aaatgccgga tatcatcttt gccggttccg atacccgtaa gcagctgaat attgcagaag
    13381 tcacagtgat tttagacaat agtgatcatt atctgccatt ggagtacaat gagatcagtg
    13441 taacccgccg ttatcgacgg actggggaaa gcgagttttt catcaataaa caaagctgca
    13501 gattaaaaga tatccaagaa ttgttcttag attctggatt aggaaaagaa tcgttctcaa
    13561 tcatttctca aggaaaagta gaagcgattt tcagtagtaa accggaagac cgtcgtggga
    13621 tctttgaaga agcagctggt gtcttgaaat acaagcagcg gaaaaagaaa gcagaacaga
    13681 agctttttga aacagaagac aacctcagcc gcgtccaaga catcatccac gaattggaag
    13741 aacagttaac tcctttggcc gcccagagtg aggcagcaaa ggagttttta cggctaaaag
    13801 aaacactgac tcaaaccgat gtctctttga tggttgctga aatcaagaca gcaaaaaaag
    13861 attgggacaa caaacaagca cagcttgcga aattcaatct agaattaggc aaactatctg
    13921 agtcgatcca agaacaagaa tccattttgg cgaagcagcg taaagaaaat gcgcaagccg
    13981 atcgtctgat cgaaaaaaat cagcaagtat tattagatct ttcggaaaaa ctgaagcaga
    14041 cagaaggaca aaaagatgtt ttgcaggagc ggacaaagca tacacaaaaa agctcgcaag
    14101 aatatcaaac ttcattagca gaagcacaaa aaaaggttaa acattttgaa aagcttcaag
    14161 aaagcttgat gaaagcagca gcagaaaaag aaacagagat ccaaaaagct gaagcaaatc
    14221 tcataaaaac acagcaagaa ttagaaaaat accaaaaatc aacgaaagaa ttattggcag
    14281 aattaagaga tcaatatgtt gatttgatgc aagaacaagc agctgtagga aacgaactga
    14341 agtatctaga acgtcaatat atacaagaaa cagcaaaaag caagcagacg ttagccaagc
    14401 aatcggaagt agaagcatca gtagaccgat tgatcttaca aaaagaagaa ttgacacaaa
    14461 agcaggcgca gctaaaatca tctttaactg aaacaaaaga gaagttagaa atgatccaac
    14521 aaaacgggaa aaagttccaa gaagcattag caaaagaaca gcctaaaatg tatcaattaa
    14581 tgaatcaagt ccagcagcta cgtgctcgta agaaaagcct tcaagaaatc caagaaaact
    14641 attttggttt ttaccaaggg gttcgattgg tcttgcagca taaacagcag ctttcgggta
    14701 ttgtcggagc agtggctgaa ctgatcgatg ttcctgcaga tttcacgtta gctatcgaag
    14761 cagctttagg tggggcagcc cagcatgtca tcgttgaaaa tgaaaaagat gctcggcaag
    14821 ccatcacgta tttgaaacag caaagaggag gaagagcaac attccttcca ctgacgacaa
    14881 tcaaaccaag acaattaccg gctcatatat tgacacaagc agcagctgta gaagggttta
    14941 tagggattgc cagtgaacaa gtcagttatc ccgatcaaat ccaaactgtg gttcaaaacc
    15001 tgctaggaac gatcctttta gccaaagact taactagtgc caatgcaatc gcgcaaacga
    15061 tccgctatca atatcgagtg gtttccttag aaggtgatgt gatgaatgcc ggaggatcca
    15121 tgacaggtgg agcgaataaa cgaggaaacc aaggcagttt gtttgtacaa aatcaagaat
    15181 tgaaacagtt gactagtgaa tttgaagaag cagataaaca actccaagca caagaaaaaa
    15241 aagttcagga gctgcaacaa gaaacagctc gtttagccga agaacaagaa gttttgcgta
    15301 cacgaggcga acaattacgt tttgaagaac aagaagcgac aaatcagcta caaaacatca
    15361 taaatgaact tgaacgtttt gaaaaagaaa agcaaatctc aacttttgag acacgtgaac
    15421 tgcagcagtt tatcgaagat tacgaaaagc aaacgaacga attgaaagac aaacaaacag
    15481 atcttgaatc gcaacgacaa aaaatcgacg aagaaatcaa atcgttaagt caagaaagcg
    15541 accagatgga agccagaaga gcacaagtac aaagtcaaaa agcacaagaa caagcagatc
    15601 ttgctgtctt gaaagagcag tttaatcatc tgcagatcca gctccgcggt gcccgagtcc
    15661 aaaaagcaga ggcaaccgaa cgtcaagaag cgatagaaaa gcagttggcg acattgacag
    15721 cagacttttc tgatcatgaa gtcacagaag aaagtttgga aaaacaaatt aacgaattat
    15781 ctgctcagcg agaaacatta aaagccgagt tggcaaaagc caaagagcag cgagatagaa
    15841 ggcaaaagga gatcgataag cttgaagcag tcttggcaga gcgtaatcgt gagcaaaaag
    15901 cacgactgtc ggaacaatcc aaattagaag tccaaaaaga ccgagctgaa atgattttag
    15961 ataatcattt gacctatctg cagtcagaat atcagctgac atttgagaaa gcttcccaag
    16021 actatcaaga aacaaccgat atcgaagaca gtagaacaaa ggtttcatcc ttaaaagaac
    16081 agattgaaaa actgggacca gtaaacttaa attcaatcga acaatacgaa caagtcagtg
    16141 aaagacatac attcttagca acacagcgag atgatctgtt agcagcgaaa aaccaattgt
    16201 ttgaaacgat ggatgagatg gatgatgaag tgcggacaag attcaaagaa gtatttgaag
    16261 ctatccgtca agaattcaaa attgtctttc caaatatgtt tggcggcggt aaagctgaat
    16321 tagtactgac ggaaccaact gatttgctga aaacaggtat tgagattgaa gtacaacctc
    16381 ctggcaaaaa actgcaaagt ctaagtttgc tttctggagg agaacgagca cttacagcca
    16441 tcgcgttgct cttttccatt atccgtgtct gtcctgttcc gttttgtatc ttagatgagg
    16501 tagaagcagc gttagatgaa gccaacgtga aacgttttgg tcgttacttg agcgatttcc
    16561 aagatgacac acaatttatc gtagtcacac accgcaaagg aacgatggtc gcagcagatg
    16621 tactgtacgg agtgacgatg caggaatccg gtgtatcaaa aatcgtttct gtacggatgg
    16681 aagatatcaa tgaagaggcg aaaatcaacg aaacgaaagt gtaggaacga aaatgatcaa
    16741 attagtagca atcgatttgg acggtacatt attaaatgaa caaaagctga tttcagatga
    16801 gaataaacaa gcattagcac aagcaaaaca gcaaggggtc aagatcgttc tatgtacagg
    16861 acgcccctta gctgcgatgg cgcattatct gcaagaatta ggactggtag atgaaggtga
    16921 ctacagcatc actttcaacg gcggtttggt acaaaaaaat gatactggtg aaatcatgga
    16981 aaagaaagtg atggaagtat cagatatcca acgcttatac aaactggctc agcaattgga
    17041 tctgccacta gatgttttgt ctgacaatgt ggttttacag ctgcctagtg ccccaaggaa
    17101 gcaatccttg tacaatgtgt tgaataagct gctgcaattc caaccagcgg atataagcga
    17161 tattacagat gaatttgtgc ttaataaggc agtaatcgct tatgaccaag aagaattaga
    17221 tcctaaaatc aaagaaatac cagcagaatt ccatgaaatg tatgagatca tcaaaacaag
    17281 aagtatgctt ttggaattta tgccaaaagg tgttacaaaa gcttatggta tttctttatt
    17341 ggcaaaagat cttggtttga aacaagaaga aatcatggcg atcggtgatg aggaaaatga
    17401 tttaccgatg atccaatatg ctggactagg cgtagcgatg gagaatgctg tgcctttcgt
    17461 caaagaagca gcagatttcg tgacagccag caatgaagaa aatggcgtag ccaaagtgat
    17521 ccaaaaattt gtattagagt agggggagtc aaatgggatt ttttgataaa atcaagcgtg
    17581 cttttactgg cgaagagaaa acagaagaag ttgaagtaca ggagaagtac gacaagggtt
    17641 tagaaaagac gcgtaaaaca tttggtgaac ggatgaatga gttgtttgcc aattttcgaa
    17701 cagtcgatga agactttttt gaagaattag aagaaacact tatcggtgca gatgtaggtt
    17761 ttgaaacagc gatacgaatc accgaagcat tacgtcaaga agtgaaactc agaaatgcga
    17821 aaaaatcggc agaagtccaa aatgcgatca tcgagaaact tgtggatctc tatgaagcgg
    17881 aaggaatcaa tgaagtaaat gaactaaata tccaagaaga cggactaaca gttattttgt
    17941 ttgtcggtgt taatggtgta gggaaaacaa caagtattgg taaacttgcc catcagttca
    18001 aaaacgaagg taaaaaagta ttgatggcag cagcagatac cttccgcgct ggagcaatcg
    18061 accagttagt tgtctggggc gaacgcgctg gtgttgaagt tgttcgtggc aatgcaggag
    18121 gcgatccagc agctgtcgta ttcgatgctt tggaaagagc aaagacagag caggcagatg
    18181 ttttactagt tgatactgca ggccgactgc aaaataaagt gaacttgatg aacgaattag
    18241 aaaaaataaa acgagtaatc cagcgggaat taccagaagc accccaagaa acacttcttg
    18301 tattagatgc aaccactggt caaaacgcga tggttcaagc aaaacaattc aaagaaacaa
    18361 cagatgtgac cggtcttgtt ttgacaaaat tagatggtac agcaaagggc ggtatcgtac
    18421 ttgctatccg taatgaattg catttaccag tgaaattggt cggtcttggt gaaggaatcg
    18481 atgacttgga gccgtttgat ccaaatgatt ttgtcgttgg attgttcaaa ggattactaa
    18541 aagaagaata acccatgtct aaaaagcagg agctgtgaca aaagttgcag ctccttgttt
    18601 gaatgattgt ttttaacata gctgtgtttt tgatgccttc atttttatac ttatttgtaa
    18661 gcggttctca aacaatttcg ttctttttct tagacttttt gattataatt agattcatat
    18721 gaattggagg tcacttaatg atgaatttta agcaattatt attgcatgtc aatgcgcgcc
    18781 cttttatcga ccaagctcga tttggtatag aacgggaagg acagcgggtt gatcttgcag
    18841 gaaatctagc aaaaaccgat catccagcaa tctttggcga tcgatcctat cacccctata
    18901 tccaaacaga ttttagcgaa acacaaacag agatgatcac ccctgttacc gattctattc
    18961 ccgaattatt tcagtatctc gctgctgttt atgatgtgac tgctcgttct ataccgaaag
    19021 aagagatgat ctggccatta agtatgccac ctgccttacc ggaaaaagac gaagagatca
    19081 ttattgcaaa attaaaaaat ttcgaagatg tcttgtatcg acgttattta gcaaaaaaat
    19141 acgggaagcg aaaacagatg gtaagcggta ttcatttcaa ttttgaattt ggcgatgagt
    19201 tgctaagaac attgttcagc catcaggaag aatttcaaga tttttccgaa ttcaaaacag
    19261 aactttattt gaaaacagcg agaaacttta tgcgctatcg ctggatgatc acttatttat
    19321 tcggtgcttc accaatgagt gagaagaact actttttaga tgaatcacat ccgcaagaac
    19381 ctgttcgcag tattcgcaac agtgcgctgg gctatacgaa tcatccaaat gtgaaagttt
    19441 cctatgcttc tatgaaacag tatttagcag atatcgagcg aatgatcgaa gaagggaaac
    19501 tttcggagga gaaagaattt tatacaccgc ttcgtttccg aggcggaaag aaagtcgcag
    19561 atttagcaac aacaggtgtt cgttatattg aattgcggaa tatcgatttg aatccttatg
    19621 caagattggg tatcaatcca gagcaagttc ggttcttaca attattcctg atgtacatgt
    19681 tatggacaga agaaaaagaa gactgtgacc aatgggtagc agaaggtacg actcgaaata
    19741 acaaagtagc actagaacaa ccatcagatc aaacagaatt tcatcaagaa ggtagagaaa
    19801 ttcttgaagg aatgaagcaa atgctggttg aattggattg gttggattct ctttatttag
    19861 tagaggaagc attgactcaa atggatcatc ctgaacaaac gttagcagcg aaactctatc
    19921 aagaagcaca gctatcgagt cagcaagaag ttgctgtggc attgggacat caatattata
    19981 aagaaagcca tgaacgtccc tatcagttgg ccggttttcg cgaaatggaa ctttctactc
    20041 agatatttat gttcgatgcc atccaaaaag gtgtccaagt caaagtatta gatgagtcgg
    20101 atcaattttt gcgtctgcaa ttccaagacc atgtagaata tgtgaaaaat gcaaacatga
    20161 caagtaagga cagctatatc gtcccactta tcatggaaaa caaaacagta acgaaaaaag
    20221 tcttaaaaga agctgggttc cgggtaccag gaggcgcaga attttcatcc atggaagaag
    20281 cggtaaaagc ttatccgaga tttgccgagc aggcatttgt gatcaaacct aaatcaacca
    20341 attacgggtt aggtatcacg attttcaagg aaggagctag tctggaagat tatcaagcag
    20401 gtttagccat tgcttttcgt gaagacagtt cggttttagt agaagaattt atgccgggaa
    20461 cggaatatcg tttctttgtg atcgacggag aagtccaagc catcatgttg cgagtccctg
    20521 ctaatgttat tggtgacagt atccgaacag tgaaagaact tgttgaagaa aaaaatagtg
    20581 accctttgcg tggaacgaat catcgtgcac ctttagaatt gatccaatta ggtgaattag
    20641 aacaattgat gttgaaagaa caagggttga cgatcgaatc agttccccaa gccaatcaaa
    20701 ttgtttatct aagggagaac tcaaatatca gtacaggtgg tgattcgatc gatatgactg
    20761 atgaattttc tgaagcttac aaaaaaatcg ccgtttctgc ggtagaagca ctaggagcta
    20821 agattagtgg tatcgatttg attataccag ataaagaaat cgatcctaca actgataaaa
    20881 aagcatatgg gatcatcgaa gcaaatttca atccagccat gcatatgcat gtttatcctt
    20941 ttgcaggaaa aggcagaaga ttgacgatga atgtattgaa attgttatat cctgaagtat
    21001 tttaaaagaa aaagcttttg acagattgtc ctatttgatg tcaatctgat caaaagcttt
    21061 ttttgcatgt aaaaatttat gtgttttttc gttttaaaat aaacgatccc agaagctttt
    21121 tttctttttt ggtttttctt tttcagctgg cgaagagttt tcttctgggt attttttttc
    21181 gatatcaatc acaggttcat ttactgcttc ttttgctgtt aagagcaatc cgaattcctc
    21241 aggatttgca gtgacgtaat cgttgactac agtgaaacgg ctattgtttt tcgttgctag
    21301 ctggatatat tggttttgga cagaaagggg tagagcccca ttcaagagta tggaagaatc
    21361 tggatgtttt ttgagttctt ttaaaaagtt ttctttgttt tttggttgga tcatttcagc
    21421 aacagtcata ctaagatagc agcgttccct gaatgtccct aaatatttat gctgttcttc
    21481 agggttcact aaaggtgtac cataaagtcc cttatccaaa tggcttttta catcatcttg
    21541 tgccataaaa aataccctcc ttcttctctt tgtctttctt acgattatag catattccca
    21601 ataataccta aaacatatga aaaagcggtt gcttttgttc aacgattcac ttaaaataag
    21661 aatgaaaatc attctcaatt agaaaggtgg attggatgcg ctacggacga ctagtagcat
    21721 tattagttat tttaagtagt atttcattgt tcatcggagt atcgaatgta caattgtcag
    21781 aactgctcca gttagatggg caaagcttac aaatcttgct agtgactcgt ttgccacgaa
    21841 cgatttgttt gatccttgtg ggagcgacca gtagtatttg tgggttgatc atgcaacatt
    21901 tgacccaaaa taaatttgtt tcaccaacga ctgcgggcac gatggatagt gcgcgtttag
    21961 gtattttagt ggctatgatt ttcctacctg gtgcatcctt gattgtccga tccttgacag
    22021 cattttgctt tgcttttgca ggaacattgc tttttttatc ccttacgcga ttgttcctcc
    22081 agaaaaatca attgatgctt ccgttgattg gagtcatgtt agggaatatc attggttcag
    22141 ttgctacttt ttttgcttat caattccagt tggtacaaaa tatgacttca tggcttcaag
    22201 ggaattttgc gactgtgata cgaggaaatt atgagttatt gtatgctgtt gttccattgt
    22261 tgctcttggt ttggctattt gcttaccgat ttacgatcat tggtctgggc gaatcgtttg
    22321 cagtaaatct gggaatagat taccagaaga tgcagttttt aggcattggc ttagtatcat
    22381 tggctagtgc agtcatgttg atcatggtag gaagcattcc ttttatcgga gtgatcattc
    22441 cgaatcttgt ctctagaata tatggagatc aagtgcacaa aacaattggc gtaactgctt
    22501 tagcaggtag tttgttctta gtattttgcg atcttgtcgc tcgcgtgttg atttttcctt
    22561 atgaaatacc agtcagtgtg atcgttggaa tcatcggtgg ggctatcttc ctttatcttc
    22621 tgataagggg acgacgatat gcttaaaaaa aagccatcta tttttattgg tatgcttgtt
    22681 cttgttttat ttgttacagg aatgtacttg acgatccata catatggaaa ctggcatttt
    22741 gcatgtgcat tgaggagtaa gaaaattttt gcatttatcc ttgtctcgct agctgtcagc
    22801 agtgccacaa tcagctttca gacaattacg cataaccagt ttttgactcc ttcagtattg
    22861 ggacttgagt cgttatatgt ccttgtccaa acggtccttt tcttctttat cggaggagtg
    22921 acgatgcttc agcagacagg tacagttttt ttcttattga acgttgggct gatggtcggg
    22981 ctgagtctat tgttattttt ctttcttttg aaaaaaatga gagcgaatct ttttttatta
    23041 ctgatgtcag ggatgatttt aggtacgctt tttggtagtg tcagtacctt tttacaagtc
    23101 atcatggatc cgaatgaata tgatttgctt caaggcagat tatttgcaag ctttggcaat
    23161 atccagagtc agtatctttt gcctgcttct ttgatcatta tcggctgctg gtacctgtta
    23221 tttcgatatc gacatgtgct agacgttttc catctcggaa atgaacaagc agtaaacctc
    23281 gggatagaca cagatcgttt tgaatttttg attttatgtt gtgtcagtct tttgacaggg
    23341 acatcaacgg ctcttgtggg tccagttact tttttagggt ttattgtcgc aaatatcagt
    23401 tatcgtgtct tttctactta tcgtcatggt cagctattta tcggaagctt tctggtagct
    23461 atgctgtttc tgacaggagg acagctcgca gtagagcaga ttttcggctg gaatacaaca
    23521 gtgagtgtcg tcgttgaatt tgtcggagga atttattttg tcacaaaatt gattcgcgaa
    23581 aggaagagta tcctatgatt gatttacaaa aggtcaataa actgtaccat ggaaaatcag
    23641 tggttaaaga tgtacagtta acgattcctg aaaagaagtt gactgctttt atcggtccaa
    23701 atggtgctgg aaagtcaacg gtgttgtcga tgatcagtcg tttgatacca aaagatacag
    23761 gtgaaatcta tttagaccat aacgaagtaa aggcttggaa atccaatgaa ttagccaaaa
    23821 aattatcgat cctacgacaa tcgaatgaaa taaatgtcca aatcaccgtg aaagaattag
    23881 tagaatttgg acgatttcct tatacgaaag gaaaattgac gacaaaagac gaggaaatcg
    23941 ttcaggaatc actggaacac cttgggttag aagaaatagc ggatcaatcg attgacactc
    24001 tttcaggcgg tcaactccaa cgtgcgtata ttgcaatggt gctagcgcaa gacacggatt
    24061 atattttact ggatgaacct ttgaacaatc tagatatgaa ttttgcggtt caaatcatgc
    24121 agattttaaa aaatttagta caagaaatcg ggaaaacaat tgttattgtt cttcatgata
    24181 tcaactttgc tgcaagttat gcagacgaaa ttgttgcaat gaaagatgga cagttattta
    24241 cgcaaggaac gacccaagaa atgataaaaa aagatgtatt ggatgaatta tacgaaatgg
    24301 acatacggat ttgtgagatt gaaggccgca gattctgtct ttatttctaa aactaacatt
    24361 aggagagaag agatgaaaaa attagtagta ggtatcttga ttctagcatc atttggttta
    24421 gcagcatgtg gaaatagcaa caacaccagt caggcagaca caaaaaaaag cagcacacaa
    24481 actgaaacta cattgacgat aacagatagt aatggagacc aaatcgaggt gccaaataat
    24541 ccagaaaaag tcgtcgtatt cgacaatggt tctttagata cgatggatgc tcttggtgtt
    24601 ggagataagg tagttggagc agctacaagc agccttcctg agtatctttc atcctataaa
    24661 aaagtagaat cagcaggtgg aataaaagaa ccagacttag aaaaaattaa tcagcttcag
    24721 ccagatctaa tcatcatttc tggccgacaa agagattttc aaaaagatct aagtcaaatt
    24781 gctccgacca ttttcctttc tttagatgcg aaaaatccat gggaatcttt ccagcaaaat
    24841 gtcactgctt taggggagat ctttggtaag caagaagaag ctaaaactca attagaggaa
    24901 ttatctagtg caatcgatca gacgaaaaag aaagcagaag ctactgacaa aaaagcacta
    24961 gtcacacttg tgaatgaagg gcaattatca gcctacggat ctggttctag attcggtttc
    25021 atccatgatt tatttggctt tgaacaagca gatgatcaaa tcgaagcatc cactcacggt
    25081 caaagtgttt cttatgaata tgtgctggaa aaaaatccgg atatcttatt tgtagtagac
    25141 agaacaaaag ctattggtgg agacgacagc aaagatgata tttctgccaa tgaactggta
    25201 gcacaaacga atgctggtaa aaatcagcag atcatctccc ttgaaccaga tgtctggtat
    25261 ttaagcgggg gcggtttaga atcaatgaaa ctgatgatcg aagatgtgaa tcaagcattt
    25321 aaatagcata aataacagag gattaaactc tcatactatg taacaataat gtttcttaca
    25381 ttcttaaata gcaagagcct atcctcaaag aagttatcaa aaaccatccg taaaatcgta
    25441 ttgatgattt tacggatggt ttttatttat ttgagaaatc ttttttcaac gtgaattcat
    25501 tatctacatc atacgggttg attctagaat ggggagtggc aaaaaatagt gtgatccatt
    25561 ttttcagcat caagatcgcc tcctttgatt tgaatcaaag gtacaaagcg aaacttagat
    25621 aaaacttaaa tttagatatt tttattttgt accgatctct tctttaaacg atgttccatc
    25681 tgctgcttct cggcaataag tccactagac tagaaaatat gagaagctat tttctgtcca
    25741 gcgttcttat tactcaaaac aaatcgcagt tgtcacaatc tcttctaaac gatgttccat
    25801 ctgctgcttc tcggcaataa gcccactaga ctagaaaata tgagaagcta ttttctgtcc
    25861 agcgttctta ttactcaaag caaatcgcag ttgtcacaat ctcttctaaa caatgttcaa
    25921 tcagctgacc ttcggtatta gctcaaagca ctgatttcac aacctctatt taggcatgtg
    25981 tctcagcaag cggttgatag ttgatgatct gcatttttcg ataaaaatcg gcgggaagct
    26041 tactccaccg ttttagtaat tgatagaaat aactctcttc tgctttgatt ttatactcgc
    26101 ttaatatcaa tccaaaacta ttcgtttttt ttgaagctaa ttttttataa atatccaatg
    26161 ctttggtttt atcttcattg acaaagagtg tttttacttc aatataagaa ccctcgatat
    26221 tttgtgtttc ttcgataaaa tagatgtttt ttatatattc cttcatagtg attcctccaa
    26281 agaagtcttt tatttgataa gtttactcta ccgaagagtt gttaataatc cgtgaacaaa
    26341 aagtaattct ttttccaata gttgttaaag gttttttatt ttttatagta ttttttgtta
    26401 tttaagaaag gaataaggtt aaacaaataa caggttgctt tttgggtctt tttccgttca
    26461 agaaactgaa cgaagaaaag ggaaagttgt ctcttttgtg ataagagaaa tcataataaa
    26521 cgaatgactt actttccaat aaattgagaa cagtcagaac cagacaaatt agttgcatct
    26581 gatgaataaa gtgattattc tatgacatga ggtgatacag atgaaattag aagtgacaaa
    26641 agaagcacaa gaagtattga agaataaatt agaagcagac gatcaacttt tattagatat
    26701 cgaaaacggt gacggtccat ttgcagaaag ccaagtcagt tgccaattgg atacggcatt
    26761 tcgattgatc attgtcaaaa aagatacaca aacagatcta agtctttatt ctgtagtggc
    26821 tgatacacca gttggaccaa tcaaaatcaa agacagtgca atactttaca tggatgatcc
    26881 atccacactt gctttagaac caacttataa tagtttacaa ttaaaaggac caagcggctt
    26941 gagaaaaggc aacttgcaag ttgttcgaaa agactagagg gataaaatgg gaaaattggc
    27001 gatcatcagc gacctacatg tggacatcaa taaattcggc gaatcagagc tgatgcagct
    27061 ttggcaagtt ttgcaaaagc acaagatcac tcggcttcat ctagccggag atacggctaa
    27121 taaagtagac cgttgcctag ctgttgtcga atttttcgtc aataaaggat tatctacgac
    27181 atttcatttc ggcaatcacg agttggcaga tgtgcccaat gaacagatga tggaccattt
    27241 tccagaccca cattttttaa atgaacggta tattgcttta aatgagcaga ccgttctttt
    27301 agggatgaat ggctggtacg actatcagtt ttccgaattg aaagattacg aggcaatcgt
    27361 acgaatgaag cgtttgtatt ggtatgatcg gttcatccag cgtgggaaaa cagatctcga
    27421 ggtcaatgca gaaatgcttg cagctgcaaa aaatttgcta gaccagctgg aagcaaaaaa
    27481 acttgaggtg attttatcca ctcatttcgt tcctaaacgg gcatttatca tgtatcaatc
    27541 tgctccttat gagcgttgga acaaattaaa tgcattttta ggatcagaat cattcggaga
    27601 attacttgac cgttattcaa atatcaaaca agtagttttt ggccatacgc atcgtcgctt
    27661 tgaagataaa tccatcggtc acacgattta cagctgcaga ccttttggtt attattatga
    27721 atggcagctg acccgagatt ttgtcttgaa agaacagttg ctagacagct atgatcctat
    27781 gaaattacgt gtcctcttgc gtgcccatca cccagctttt caggcctatc aaacacaaca
    27841 tttgcaagag gaatttgaac aagcgatgac aatcgttccg tattgagaca gcattagtaa
    27901 tgtgtaaaag aaaatacctt gtaaaacata aggataagta agagcagtag atccatttat
    27961 cgtggatatg ctgctttttt tgcggaaaat aatcagcgaa tgtttatttg atcggatgat
    28021 ttgtaactcg cagagtacta gatgcaagcg tgtattcttt ttttgaacga taatcacaaa
    28081 aaaaaatata atatacattt tttatttaat tataattaat attgattgaa gtctaggcag
    28141 tcaagtcaca ttttgataga taggaggggg acttacgaat tattttaaag gctatgacag
    28201 ctgtatttct agattgtgtt tgcatcaagt tgcaataaaa actagaaatc aaatcgggct
    28261 gataatcatt tataactaat cgatacatag gaccaaatat aggtctatgt tcttttattt
    28321 cataagtatc gtaaaacaga attttgctat ggattaatcc aatcgactga tatgtttagt
    28381 aaataaagaa agaggaatgt caatgatgaa aaaagaaatc gtattaacag cagcaatgat
    28441 gttattcagc atggtagcaa gtacgacttt tgtttctgca actgaagtat acccaaagga
    28501 atataacaca gaaggaacga ttacatttga agcaggagat gagggtgtaa ctcctcctgt
    28561 tgatccagag aatcctgatc ccaataaacc tgtcgatcca tcagatcctc caagccctgg
    28621 aacaggaggg gcattatcga ttgattatgg atcaaaattc aaatttggga ctcaaaaaat
    28681 ttctactgcg gataaaacgt attatgcagc tgctgatgtg atgaacgatg ggtctagaaa
    28741 accgacatat gtccaagtga cagataggag aagtacctta tctgggtgga aattaagtgt
    28801 ctctcaacca gaacagttta aaactgccag tggggatgaa ctagttggag cacagctgaa
    28861 atttacgaaa gggcaggctg tttctttggt tgatccaaca tatacgccgc aaacggtgaa
    28921 ttctgaatta acgttgacac cgggaggaaa caatacgtta gcaattaatg ctaaatcagg
    28981 aacaggtgtg gggacttggg tctatcgatt tggtgcaaat gaaaatgaaa atcaagatgc
    29041 ggtacagctt tcggtacccg gtaaatctgt caagttagcg caacaatatt ccactaaatt
    29101 agtttggaca ttagaagata cacctaacaa ctagtttact gaaaagaact gttaaaaata
    29161 aatacgtagt catattgtct tgcgaggtaa tcgagcaaaa attgtcaaag gctaggctaa
    29221 atttgtttgg atacgaatag ttcatctagg aatttaacga tcaggaggaa aagatatatt
    29281 gaaaaagagg aggatcgtgc tcattttaag cagtctctta gtgactaata tctttccgat
    29341 catagggtac tctttggaat tgaatcagtt ggagcatgga aatagaagag acgaagaaag
    29401 caaactcact tcgacattcg acagtttaat agaatccaac cagacacaag aaagcagtga
    29461 ggatttaagt atttcgagta gtggagagac cactgacaac gtagaagctg agggttcgga
    29521 caaacaagga ctagaagatg caccaatttc tgcggttgag ctgcctgacg atcccaatat
    29581 tatttctatc gataaaattt ttcaaacacc tatcggtact agcacgtcca tattggaaaa
    29641 tgggaagtta ctgcagttga acccagcaga aaggtctcaa agaggagcta tttggtcaaa
    29701 taagaagatt agtttactct cggattttac atttaaatct tatttatact taggaaatca
    29761 acgagataga gcgggagatg gcatgacctt tacattgact ggcgatccta gaatggctag
    29821 taatcccagt caagttattg gatcgccagg tatgggaatt ggctcatatt ctactcgttc
    29881 tggccaacct tacattgcga atgcgctctc gattgagttt gatacgtata aaaatagtgg
    29941 ttcttctgat cgaatggatc gagagataac tgctgataat agagggcatg gacatgtcgc
    30001 ttttgtcacg cctaaaccaa ataataataa ttattctgga gagcatactg gcgtgacggt
    30061 agctcctaca tttctttcta atggcacatg gcgcttgtta ataattaaat gggacgcgga
    30121 gcaaagaagt cttacttatg atttggaagg tgtcggtcgc tcttctcgga ttatacctga
    30181 tctcattagg cagtttggtt ctacagaagt aaattggggg tttacttctt caacaggtac
    30241 tttttttcaa gaaaatgctg tcgctgtcac gcgacttcca tcgaatgtca catcaacagc
    30301 tgatgtaaaa gtcaacgata gtgattatgg ggccatcgct gaagctggag gaaacgataa
    30361 gataactatc agaaatacat tgaaggtcga gggggatttt cttgaatata agaaaatgca
    30421 gactagtatc gacctcccac aagaattaac tattcccgag catatcttga tcaatggaaa
    30481 tcaaattgat gccaaagata tcttgataac cggaaatcga gtcacgctaa atctgacaga
    30541 ttattttatt gctggagata cgttaataat cgaattggag acaactttgc aggaagtaat
    30601 tcctgaaaaa gtactcacta tgcattttga ataccttgaa aacgggaatt tgattgagca
    30661 atcgaatgaa ataaacctaa agatcgcaaa gctgaaggag atagatatcc atgtttatta
    30721 taaagataaa gacagtcaga ttgatattgc tgaaatgaaa acaatcactg ggaaaatagg
    30781 agagaagtat aaagaaactc ctattgatat tgaagggtat gtttttgact cggattcagc
    30841 gaatacagaa ggcatcttct ccgaaaatac agaggatatc tatttttatt acagaaaagg
    30901 caaactttat ctctcaactg ctcctcaata tgtcaatttt ggaaaacata aaatatccag
    30961 tagaccatta agtgaattcg gacagttttc aggtgggttg aaaattatag atgaacgggc
    31021 ttttggaagt tggaaattac aattgaaaca agagaaagtt cttacaaatg gagattttga
    31081 gcttccagaa gctctttcct ttgttacctc tgaaaataca acggttatag ggacatccgc
    31141 tgttacgatt ttcgaaagtg atcaaaaggg tgaaagcgat ttgtcctctc ttttagattc
    31201 tacagctcat cgtggaatca gaataaatat tcctgtagaa aaccaaagaa tcggaacctt
    31261 tgaggggaaa ctatcttgga tacttgcaga tgtcccagga aattaacagg aagagaagaa
    31321 tataacaaaa ttattgagtt tcaagaaggg cacaggtacc ataggctctt gtgtcccaca
    31381 gagacaagat tttgttatag tcttctctta catatgatta aaggagcgat tttcttgaga
    31441 ccaaaacaac aattatttat aacactccta gttattttag gggcactttt taacaatata
    31501 tcagtgtcag ctagtgcatt taatttttca gtgacgccga taacttcaga gaatcaaatc
    31561 gacaagagaa aaacttactt cgatcttcaa cttgatccgg atcaggaagt agaagtaaaa
    31621 gcggaattga gaaatgatac agaaaaagaa gtgaaaatcg atatttctgt gaatagcgca
    31681 accaccaatt caaatgtgat ggttgaatac ggaaaaaacg agattgaaaa agacatgagc
    31741 ctgatctttg atctggtaga ttatgtgagc tatccacaga tagtgacgct aaaacctaaa
    31801 agtgtccaaa ccgttgcttt tcatgtcagg atgccgaatg agcgatttga tggtgtattg
    31861 gctggtggga tcacattcca agaaatccag acggaaaagg accaaacaga aactaaggat
    31921 caaagcttat cgattgagaa tgaatacacc tatattattg ctttgttgat gcaacagaca
    31981 ctgaacgaag ttgctccgaa ccttactctt catgaagtta aaccagatca aatcaatgca
    32041 agaaatgtca tcttagcaaa cgttcaaaat gatcaaaaaa cctatatcaa tcaagtagtt
    32101 attgaaacaa aaattacaaa aaaaggacat tctgaagttt tatatcaaga ggaaaaagaa
    32161 ggattgcaga ttgcgccgaa tacaaatttt tcttttccaa ctgctttaaa tggtcaaccg
    32221 ctaaccccag gtgaatatca ccttacaatg actgttttag gaaatgaaaa tgaaaatgga
    32281 aaattttcga gaaaaaaagg aaatactacg atcaactata ccaatcaatg ggtatttgaa
    32341 aaagatttta caatagacgg aaaagtagcc aaggaattga acacgacgga tgttacgatt
    32401 aaaaaagaca ataattggat ttatatctta gtagcttcac ttttcttatt gtttatttta
    32461 ttatgcatac ttttgtttgt tttgagaaaa aaagaagaga aagaaaagta aggttaagat
    32521 aacaaatagt aaatttttat tattgtagga tggttgggct ctttttataa caatataaaa
    32581 tataccattt tattaataaa aatgcatact agtaaatgat ataattctaa taacaggttt
    32641 gatcgcgaga aaggccaaat aaaacgctct ccaaaatgtt aaaaaaaaga caggacgatg
    32701 tatattatac aaacaagaat cgctgacaac agtgactcgg atccaggaaa tcagttttat
    32761 ccccagctag ttggtgaatg catggaaagt aatatacgta gaaagagctg atgactaatg
    32821 aattatgagt tttttttaag caaagaagat cacagaagtt attcattact taaatattta
    32881 gaagcaagct caaatttatc agagtcaata tcgaatgttc aagaagagtt gtcgctatcg
    32941 acatttctct tgaaaaaaac gatcgataag ctgaaacatg atttacaaaa gttcgatcta
    33001 gagaaaaatt tgcgagtaac cgtcagtgaa ttggatatat gtctggaaat taatggaaaa
    33061 tgttctagca aagctctgtt atctcgttat ataacggatt ctttatctat gaaaatggta
    33121 ttggctttct ttcaggaaaa atatatctca gtagaaaatt ttgctaaaca gcatcacgtg
    33181 agttattctg tggcttataa agtattacaa agactaaaaa gaaatctaaa aaaatatcaa
    33241 attttttttg aaaagagaaa gtt
//
LOCUS       DASHHB010000030        31000 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.030, whole genome shotgun sequence.
ACCESSION   DASHHB010000030 DASHHB010000000
VERSION     DASHHB010000030.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 31000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 31000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..31000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.030"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(391..2466)
                     /locus_tag="U2K71_001554"
     CDS             complement(391..2466)
                     /locus_tag="U2K71_001554"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HEM7315639.1"
                     /translation="MAKDLLLEIGLEEMPAHVVTPSMKQLEEKTAKFLDENQLTYDTI
                     ETFSTPRRLAIKVTNIPERQADSEEEVKGPAKKIALDADGNWTKAAEGFVRGQGLTTD
                     AITFRELKGVEYVYVTKYTHGKEAKEVLTGLADVIKSLTFPVTMHWAEYDFEYIRPIH
                     WIVALLDDEVIPFEILDVKTGRASRGHRFLGEDVTFAHAKEYEEKLNEQFVIASPQKR
                     QEMIVTQANELAEKNHWVIDLDEDLLEEVNNLVEYPTVFSGDFEEKYLSVPEEVLVTS
                     MKEHQRYFEVRNDQGMLLPHFISARNGDRVKLENVVRGNQKVLTARLEDAEFFYNEDK
                     KASIDQYVEKLKTVTFHEKIGSIYEKMQRVQVIARIIGEQVGLSEEELMDLQRAAEIY
                     KFDLVTNMVGEFPELQGIMGEKYALLKGEKPAVATAIREHYMPISSEGELPQSTVGSV
                     LAIADKLDSIFSFFAVGMIPSGSNDPYALRRQAYGVVRIIESKKWAFPLSVLQETISD
                     VISKDTDRFGIGLSEGQQQVIDFIKGRLRQLLTTKNIRHDVIEAVLNAEQKDLTKVFA
                     AAQLFKQHLADEDFKPSMEALTRVVNLAKKAELEKQSEVDPELFENEAEKELHKAVEA
                     MKKSFADQTINENYDQLVSLRPLIENYFDQTMVMAEEPAVRKNRLTQLSQIAAMALSL
                     ASLDQIITK"
     gene            complement(2468..3385)
                     /gene="glyQ"
                     /locus_tag="U2K71_001555"
     CDS             complement(2468..3385)
                     /gene="glyQ"
                     /locus_tag="U2K71_001555"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit alpha"
                     /protein_id="HEM7315640.1"
                     /translation="MSKKLTVQDMILTLQKFWSSNGCMLMQAYDTEKGAGTMSPYTFL
                     RAIGPEPWNAAYVEPSRRPADGRYGENPNRLYQHHQFQVVMKPSPENIQELYLESLRL
                     LGIDPLEHDIRFVEDNWENPSMGCAGVGWEVWLDGMEITQFTYFQQVGGLPCKPVTSE
                     ITYGLERLASYIQEVESVYDLEWADGVKYGEIFKQPEYEHSKYSFEVSDQDLLLSNFD
                     RFEKEAKRCIDENLVHPAYDYILKCSHTFNLLDARGAVSVTERAGYLSRIRNMARAVA
                     KIFVAEREKLGFPLLNKNTANPENMKEAE"
     gene            complement(3769..4581)
                     /gene="recO"
                     /locus_tag="U2K71_001556"
     CDS             complement(3769..4581)
                     /gene="recO"
                     /locus_tag="U2K71_001556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HEM7315641.1"
                     /translation="MSQLAESKGLILSSRNYKEKDKLVKIFTESSGKMMFYIKGIHRQ
                     NQPLAPALLPFTEAVYIGNFRSEGLSFLSSVKDVHAFRNIQEDIFLSGYGTYLLNLVD
                     AAIEDHQYDPHLFQFTQQALQRMDQGDDAEIITNIFEVQILQRFGIAPIWTHCVVCGE
                     TKGKFDYSSKSGGVICEKHWPMDEHRYHADPRAVYFVRMFSAISYDKISGIHLKEETK
                     QAIRDLIDQLYDEYVGIHLKSKKFIDQMKSWAHVLKTPEKNMPEKEIPEKEE"
     gene            complement(4724..5623)
                     /gene="era"
                     /locus_tag="U2K71_001557"
     CDS             complement(4724..5623)
                     /gene="era"
                     /locus_tag="U2K71_001557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HEM7315642.1"
                     /translation="MPEHKSGFVAIVGRPNVGKSTLLNRIVGQKIAIMSDKAQTTRNK
                     IQGVYTTPEAQLIFIDTPGIHKPKHRLGDFMVEAAYSALKEVDAVLFMISADQKRGRG
                     DDFIIERLKNVHSPVYLIINKIDKIHPDELLGIIEDYSTQMPFAQVIPISATEGNNVE
                     RLMDVLVSEMPEGPQYFPDDQVTDHPEYFIVSELIREKVLFLTRDEVPHSVAVVVDSM
                     KRNENDKIHIQATIIVERDSQKGIIIGKGGKMLKQVGTKARKDIENLLGDKVFLELWV
                     KVQKDWRDKKVYLQDFGYRKDEY"
     gene            complement(5638..6033)
                     /locus_tag="U2K71_001558"
     CDS             complement(5638..6033)
                     /locus_tag="U2K71_001558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HEM7315643.1"
                     /translation="MDSKDKNVEKNKHFLMSVEFAVQGVKTVFEEERNMRKHVAFGIL
                     ALFMGVIFQLDRIEWLWLLLAVFLVWIVEIINTVFENVVDMFTDFHFHPIGKKIKDMA
                     AGAVLVTACFAVIIGLILFVPKIYHLLFN"
     gene            complement(6017..6493)
                     /gene="ybeY"
                     /locus_tag="U2K71_001559"
     CDS             complement(6017..6493)
                     /gene="ybeY"
                     /locus_tag="U2K71_001559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HEM7315644.1"
                     /translation="MDITFMDETNQVSEEKMKEIDDLLQFAASYLKLPEETEMSVTFM
                     DNAAIQVINRDYRGKDAPTDVISFALEEEGEDEIPVIFEDGENPLPRVLGDIMISIER
                     AAEQAEAYGHSYDRELGFLAVHGFLHINGYDHMTPEDEKEMFGLQKEILDAYGLKR"
     gene            complement(6510..8702)
                     /locus_tag="U2K71_001560"
     CDS             complement(6510..8702)
                     /locus_tag="U2K71_001560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HEM7315645.1"
                     /translation="MLNKTLQKLQEKLGKAALPFLLFLFFIIVCGVTFSSVKQKANDF
                     REGQVAEESIRANKTIENTEETEQRRKLAAEAVTPEYTYQKDLADDQNNRIKQLFELI
                     DKTNDTINKSYNDKVDKAKEDENIPKPTTEERIATLKKNFENVNAENVAFYQKLPNNF
                     YSTIFEMTETEIHTVRDESLKLIDEQMSKQVRESELEAFKQEAEDQIQYLNVTTEQQQ
                     MIRYLVDQGIVVNDVLNEKKTEELKQSAREAVQPVMIFQGEIIVREGNQIDASAMKKL
                     ELLGLTNQTTSIFPLIAMVLAVLLQIGVLIYNSLQFQNAGERTKYVLFYVTAMSVSIV
                     LMKFFQLFQTEQATYIPLFYPAAFAPLVLSFFLNRRAGIMAGIFQAVSALFIFYDSIG
                     TNFLTIILMSYVFSGLMATIVRRKRISEQGLSAAMWVIIFPVMMDVILIVYQGMSFGD
                     ATTWLMLVCGFTGALFSFLLTIGLHPYIELMVNDDSVIVLNELSNPNHPLLKQLLEEA
                     PGTYHHSMMVANLSANAVAEIGGRSLLTRVACYYHDIGKIKHASFFVENLPSGAENPH
                     NFLLPEDSKHIIFGHVTDGAKILEDYNMPQMVIDICKQHHGTTLMRYFYIKAKERNPE
                     VTEEQFRYPGPKPQSREAGIVNIADSCEAAVRAMDHPTSEKIESFVHNLIEERISDGQ
                     LDDSGLTLKEIRKVEKSLISGLSSTFHSRIKYPKMKTEVEKIKEEQEE"
     gene            complement(8726..9697)
                     /locus_tag="U2K71_001561"
     CDS             complement(8726..9697)
                     /locus_tag="U2K71_001561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HEM7315646.1"
                     /translation="MTEESSSLEIRLTAADDVSMLLGAHDKHIKLIEETTETTIHTRG
                     EIIQIIGEQSQISLAASVIRTLQELIKRGIHVSTPDVVTALKMGQKGTLDYFVEMYEE
                     EIVKDRNGKPIRVKNSGQKKYVESVAKHDIVFGVGPAGTGKTFLAVVLAIAALKKGEV
                     QKIILTRPAVEAGENLGFLPGDLKEKVDPYLRPVYDALYQIFGLDHTNRLMERGVIEI
                     APLAYMRGRTLDDAFVILDEAQNTTVAQMKMFLTRLGYQSKMIVNGDTSQIDLPRGTT
                     SGLVHAERTLKQIKKIDFVNFEASDVVRHPVVAEIIKAYEKADLHQE"
     gene            10403..11782
                     /gene="brnQ"
                     /locus_tag="U2K71_001562"
     CDS             10403..11782
                     /gene="brnQ"
                     /locus_tag="U2K71_001562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEM7315647.1"
                     /translation="MEETKTYSWKQLLMVSSLIFGMFFGSGNLIFPVHLGQMAGANWV
                     SAGVGFAISGALFPLLAILAVVVTKSDGLYDLAKPVGKWYAALFLILVHLTIGPFFGT
                     PRTAATAFEMAAKPFLPEKYNSLGMFLFSALFFLLAYFLTVHPTRLVKYVGKYLNSLF
                     LALLAIIFVVSLFHPMGNLNQSAADTYQTNALTNGVLEGYNTVDAVALLALSVTFVHA
                     VRDLGFKDKKVTELTAKAGSLSVALEVMIYFGLVLLGAMSLNKMSLSENGGTALSQIT
                     NFYLGNFGASFLGVMVTLGVFTTAMGLVVSFAQDFHKLFPKVSYMTWLRLTTFVSFVV
                     ANAGLDNIIQWSLPVLMLLYPLSLALILLSLTAKFFQKTPFVYQVTMLFAAVPAVLDM
                     LANSPALVSQQRVVASMLEFYHHHVPFAALGLGWMVPTLLGYAGSLLFYYAYRLSGYK
                     QEANELPEE"
     gene            complement(11938..12384)
                     /locus_tag="U2K71_001563"
     CDS             complement(11938..12384)
                     /locus_tag="U2K71_001563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HEM7315648.1"
                     /translation="MSLLSRLNDDMKTAMKAKDKESLQVIRMIKSSIQNEQIKEGHDL
                     TEEEELTVLSREMKQRRDSLHEFEEAGRDDLAEKVKSEIVIVEKYMPEQLSDEEIRQL
                     VQEAITQTGASSMKEFGKVMGAIMPKVKGKADGNQVNAIVKELLQS"
     gene            complement(12411..12587)
                     /locus_tag="U2K71_001564"
     CDS             complement(12411..12587)
                     /locus_tag="U2K71_001564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010754017.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HEM7315649.1"
                     /translation="MSKTVVRKNESLDDALRRFKRSVSKAGTLQESRKREFYEKPSVR
                     RKKKSEAARKRKKF"
     gene            complement(12749..13177)
                     /locus_tag="U2K71_001565"
     CDS             complement(12749..13177)
                     /locus_tag="U2K71_001565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEM7315650.1"
                     /translation="MKKQTLIEQSLETLKNNGFKYTKKREALLTYLVKRNRYVSAKEV
                     FDYMNQEFKGVSYDTIYRNLHDFAEIDLLEETDLNGEMKFRFHCCQGEIGHHHHFICT
                     VCGTTKEIHMCPMNYFEEQLGGCTIEGHRFEIFGRCENCQ"
     gene            13269..14129
                     /locus_tag="U2K71_001566"
     CDS             13269..14129
                     /locus_tag="U2K71_001566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="kinase/pyrophosphorylase"
                     /protein_id="HEM7315651.1"
                     /translation="MSSDSTEIVTFFVISDSAGETATKLAQATMAQYPSVEFNLFRRT
                     FVTDEKTLMQALQDALEEKALVLHTLINQELIEMTRSFCEEHQLFSFDVMTPPISEIE
                     RLTGIKPIRQPGALHLLNENYFKRIKAMEFAVKYDDGKDPRGFLEADVVLLGVSRTSK
                     TPLSLFLANKNLKVANLPLIPQAHIPKQLWEIDPKKIVGLTNNPDILNNIRKERMRSY
                     GLNPDTAYSDIEKIRAELDFANDLYEKLGCVVINVASLSIEETASLILNALDLEDHSY
                     YGTETTEETD"
     gene            complement(14213..14563)
                     /locus_tag="U2K71_001567"
     CDS             complement(14213..14563)
                     /locus_tag="U2K71_001567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HEM7315652.1"
                     /translation="MEKVVNFRSQSFFKLEVNGLPDTQKTKVEKVSQLFKMLSDPTRL
                     KILLYLKDGEQNVTAIIQAVEMEQSAVSHQLRLLRENHVVKSHREGKAILYSLDDSHV
                     LDILNQTLKHVEHQ"
     gene            complement(14579..16306)
                     /locus_tag="U2K71_001568"
     CDS             complement(14579..16306)
                     /locus_tag="U2K71_001568"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-sugar mutase"
                     /protein_id="HEM7315653.1"
                     /translation="MSWEQVYEQWVHEENLEENLKKQLNDLGEDPEKLEDAFYAPLEF
                     GTAGMRGILGPGINRMNIYTVRQATEGLARFMDTQGEETKRRGVAIAYDSRHQSPEFA
                     MEAAKTLAHHNIPSYVFESLRPTPELSFAVRHLKTFAGIMVTASHNPAAYNGYKVYGE
                     DGGQMPPADADALTKYVREVSNPLKVEVLSDEEAEHSGLITIIGEEVDAAYLEEIKAV
                     TIDHELVETMGKDLKLVYTPLHGTGKMLGERALKQAGFEQFVLVPEQAVADPDFTTVK
                     SPNPEEHSAFEYAIRLGEKEGADLLIATDPDADRLGAAVRLPNGSYEVLTGNQIGALF
                     AQYILEAHQSRGTLPENAAVLKSIVSSELPTAIAEHYGATMFNVLTGFKFIAEKIQQF
                     EEDHSHTFMFGFEESYGYLVKPFVRDKDAIQALLLLAEVAAYYKKQGKTLYDGLQTIF
                     ETYGYYAEKTISVTMSGQEGSAKISALMEKFREEAPTEFAGAKVIQTEDFKELTRTDR
                     EGNVEKLTTPPSDVLKYTLEENGWIAIRPSGTEPKIKFYIGVKGNSSEDAAEKVAAYE
                     TIIQKMTEE"
     gene            complement(16519..17445)
                     /gene="trxB"
                     /locus_tag="U2K71_001569"
     CDS             complement(16519..17445)
                     /gene="trxB"
                     /locus_tag="U2K71_001569"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HEM7315654.1"
                     /translation="MYDVIVIGAGPAGMTAALYASRSNLSVLMIEQGAPGGQMNNTAE
                     IENYPGFDSIMGPDLAYKMYEGVSRFGTENAYGIVQDIKDHGSHKEVICEDKTYEGKT
                     VIIATGCIHRKLGVPGEEEFAGRGVSYCAVCDGAFFRNKRLVVIGGGDSAVEEAIYLT
                     QFASEVVIVHRRDELRAQKIIQDRAFANEKISFMWDTVVDEIVGNEMVVTGVKARNVK
                     TGNEFDLAADGVFIYVGLDPLTAPFREAGLTNEAGWIPTDNEMKTNIPGVFAIGDVRE
                     KDLRQITTAVGDGGIAGQQVYKYLESLEETTA"
     gene            complement(17590..19026)
                     /locus_tag="U2K71_001570"
     CDS             complement(17590..19026)
                     /locus_tag="U2K71_001570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEM7315655.1"
                     /translation="MMGFRKDFLWGGATAANQCEGGYDEGGRGLANVDVVPIGEDRFP
                     VITGKKKMFEFDENHFYPAKIAIDMYHHYKEDIALFAEMGFKTYRMSIAWSRIFPMGD
                     EKEPNESGLKFYEDVFKECQKYGIEPLVTITHFDCPMHLIKEYGAWRSRELVGFYENL
                     CRVIFNRYKGLVKYWLTFNEINMILHAPFMGAGLYFEEGENEEQIKYQAAHHELVASA
                     IATKIAHEVDPENKVGCMLAAGAYYPYTCNPEDVWASRKSDRENYFFIDVQSRGEYPA
                     YALKEMERNGIQIKMEPGDAALLKEHTVDFISFSYYSSRVSTTDNELLEQTAGNIFAS
                     VKNPYLEASEWGWQIDPLGLRITMNDIYDRYQKPLFIVENGLGAVDTPDENGYVEDDY
                     RIDYLAAHIQAMKDAVELDGVDLLGYTTWGCIDLVSAGTGEMKKRYGFIYVDRDNEGN
                     GTLARSKKKSFNWYKKVIDSNGENLSND"
     gene            complement(19023..20426)
                     /locus_tag="U2K71_001571"
     CDS             complement(19023..20426)
                     /locus_tag="U2K71_001571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-glucosidase"
                     /protein_id="HEM7315656.1"
                     /translation="MAKGIKIATIGGGSSYTPELMEGFIKRYEELPIREIWLVDVEEG
                     KEKLEIVSQMSQRMWDASPYEVKIYATLDREEALKDADFVTTQFRVGQLDARIKDERI
                     PSYYGMIGQETNGAGGIFKAFRTVPVVLAIVEDMKRLCPNAWLINFANPSGMVTEAVI
                     RYGKWDKVIGLCNVPVMAMMTEPTMLGKKKEELIYKFAGLNHFHWHKVADNKGNDRTA
                     ELIDRLYDEDNGLPKNIFDIPFFKEQLKQMNMIPCGYHRYYYREEEMLEHALEEYQTI
                     GTRAQQVKETEAELFELYKDPELDHKPEQLQKRGGAYYSDAACETIASIYGNKETQIV
                     VSTKNDGAVPDLPADCVVEVTAYVGAQGARNVAFGSLPAAERGWLQVMKNMELLTIEA
                     AVTGDYGTALQAFTINPLIRSGKTAKRIMNELFIAHQQYLPQFKETISRLKAEGIEVQ
                     DELARELSSNTHVMEEV"
     gene            20656..21558
                     /locus_tag="U2K71_001572"
     CDS             20656..21558
                     /locus_tag="U2K71_001572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM7315657.1"
                     /translation="MLLREKIKEISFSPSENEVIQFLLSDSPALGEMTIQEIAERCYV
                     HPSTLIRVAKKMGFDGWVSFRNAFLAETAYLEGNFQDVDANLPFSTTDGIMTIANKMA
                     SLEQMTIQDTLSLIHHDQLQKAKQLMLQAKKIVIFASNSNTLISQDFMLKMKRIKMDV
                     TIVTTFGESFYEAYNCTSDMCGILISYTGENNVILRTAEILKQNHVPILALTSIGDST
                     LSSMSTAALRLTTRERLYSKIGNFTINTSICYLLDVLYSCVFAENYQQNLVHLISVGQ
                     KFDKRPISSAIMKEPFNFENKEVD"
     gene            complement(21599..23485)
                     /locus_tag="U2K71_001573"
     CDS             complement(21599..23485)
                     /locus_tag="U2K71_001573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM7315658.1"
                     /translation="MGKYQELASKIVENVGGKENINGLTHCITRLRFKLKNEEKANDE
                     ILKNMDGVVTVMRAGGQYQVVIGNHVPVVFEEVIKAGNLTFDEAVSTEKMRPFDMLID
                     GISGCFQPFLAILAAGGMIRGLTALLVFLGAFDRGSGTFVMFDNIGDSVFQFMPVIIG
                     LTAARKFKVNEFVGMLLGAALMNPSLSLEALSGAAEAPLTTIFSGTIFEAPIYQTVFG
                     IPWIARNYASSVIPIIFIVLLASQIQKPIKKLVPEMIANFFVPFFTVLITMPLGFLLV
                     GPAFTFATDILMAGFETLLALSPVIYGAIVGFFWQILVMFGLHWAIVPMGLMQFSVNG
                     WQNIMTPVAVVSFGQTAALTALYFKLRNPKDKAIAIPAIVSGIVGITEPAIYGFTLPR
                     KKIFIFTCVGGALGGAYSGLMNLTSWNPGGLGIFTIPNYIRPDGDLTDVINVLIGIAI
                     AMVVSFALTFFFWKEEAGETDDRQKRSGKEIVKTPIQGQIAPLNAAKDAAFAQGTLGR
                     GILIYPEKGEVRAPFDGTVMTLFPTKHAIGMVSEAGLELLIHVGLDTVQLEGKYFESL
                     VQQGERVKQGDLLLRFDKKKIEEEGYSLETPVIVTNYTDYLDILENQGETVGPDDALI
                     TALT"
     gene            complement(23582..24433)
                     /locus_tag="U2K71_001574"
     CDS             complement(23582..24433)
                     /locus_tag="U2K71_001574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM7315659.1"
                     /translation="MLIEKVLNNNVVITRDERNQEMIVMGKGLAFKKKVGDPIFDSCI
                     DKKFQLTNDGLSQQFQELAQAIPLEYLEISCEIIEYTKEALQVQLNDSVYIALTDHLY
                     TAIERHKMGIFVPNVLLYDIKQLFPKEYAIGKKTVKKISDKYHIKLSEDEAGFIALHL
                     VNAQGIQSQQDNTYLFTTTIQDILNIVRYYYKITFDEDSVHFFRFVTHLRFFLTRVLD
                     GEKSEVGEVSEELYQVIQKKYKDTFGCVEKIADFLAEIFNYQMSTDEKMYLTIHIARL
                     VHENRCK"
     gene            complement(24891..26675)
                     /locus_tag="U2K71_001575"
     CDS             complement(24891..26675)
                     /locus_tag="U2K71_001575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315660.1"
                     /translation="MDENYQSEWTKSIPLKEQKKIIKRLMKFAKPFRRTFIVAILFAF
                     ALSVINVLLPRIIQTFMDDHLAKQSATTQVILFFAGMYLFGVIVKSIIWFFQWYLYSM
                     ASLKTYQYVRVRLFEKLHTLGMRYFDQTPAGSTVSRVTNDTETLFEFWYVFLMVLTGI
                     FAVVSSFIAMFQINGEIALWTLVFLPILGIVIWYYQKFSSRIYRNMREKLSQLNTKLN
                     ESISGMRIIQQFRQEARLAKEFEQVNNEYLDTRYAMIKTNSILLSPIINFLYALAIAL
                     ALTLFGIDALHSPVEVGLIYAFTTYVQGFFNPMSQMMDFLSTFTDGMVAGSRILKIMD
                     TEEYAPQQNPGADQAITSGKIEFRNVSFSYDGEHNVLNNISFVANPGETVALVGHTGS
                     GKSSIINVLMRFYEFYEGQILIDDHDIREYPMEELRKKMGLVLQDAFLFYGDIAGNIR
                     MMNPTITDEQIRHAAEFVQANKFIDELPKGYHSKVIEHGASYSSGQRQLITFARTIVT
                     DPKILVLDEATANIDTETEGLIQEGLTKMRQGRTTIAIAHRLSTIRDAELILVLDKGH
                     IVERGNHETLLAQNGLYADMYKLQSSGV"
     gene            complement(26675..28426)
                     /locus_tag="U2K71_001576"
     CDS             complement(26675..28426)
                     /locus_tag="U2K71_001576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7315661.1"
                     /translation="MSIFRKLGWFFRQEKKHYLIGIFSLFAVALVQLAPPKVIGIIID
                     EIADKNLSIKIISWWILLLILAALLQYLFRYIWRTNIWGSAARLEKELRQQLFKHFTE
                     MDNLFYQKYRTGDLMAHATNDLNAIQNVAGAGILTFADSVITGGTTIIAMILFIDWRL
                     TLIALIPLPLLAVTSRVLGSKLHDAFRDSQEAFSSINDKTQESITGIKVIKTFGQEKE
                     DLADFEEKIEDAIIKNKRVNFLDSLFDPFITLIIGLSYVATIILGGKYVLEGTISIGQ
                     MVSFISYIGMLVWPMFAIGRLFNVLERGNASYDRVNELLHEKTHIVEQLGAVQTPAKG
                     ELNVAISSFNYPKEESPALEQIKFAIQEGETLGIVGKTGAGKTTIIKLLMREYDDYQG
                     KIQFGGCSIKEYSLNALLGSIGYVPQDHFLFSMTIRDNIRFSNPKLSEEEVEHAASLA
                     FINNEIKELPKGYDTLVGERGVSLSGGQKQRISIARALIKNPELLILDDALSAVDAKT
                     EEAILHNLKEIRKEKTTIIAAHRLSSVMHAKEIIVVDQGKIIERGTHHELLALGGWYE
                     RMWEKQKLEAKIEGGEA"
     gene            complement(28568..28792)
                     /locus_tag="U2K71_001577"
     CDS             complement(28568..28792)
                     /locus_tag="U2K71_001577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YneF family protein"
                     /protein_id="HEM7315662.1"
                     /translation="MVSTGIVVLIAIIALLIGAVGGFFLARKYMKDYLEKNPPVNEDM
                     LRSMMMSMGQKPSEKKIRQMMQQMKNQGKK"
     gene            29043..30629
                     /locus_tag="U2K71_001578"
     CDS             29043..30629
                     /locus_tag="U2K71_001578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nramp family divalent metal transporter"
                     /protein_id="HEM7315663.1"
                     /translation="MNNQSNKQFHKHRLIEHTNGLSLAEVNGTVDVPKGKSFFKTLLA
                     YSGPGALVAVGYMDPGNWSTSITGGQNFQYLLMSVILMSSLIAMLLQYMAAKLGIVTQ
                     MDLAQAIRARTSKTLGVILWILTELAIMATDIAEVIGAAIALYLLFDIPLVIAVFITV
                     LDVFVLLLLTKVGFRKIEALVVCLIFVILFVFVYQVGLSNPDWHSLFAGFIPNSHTFS
                     DSPVIGGETPLTGALGIIGATVMPHNLYLHSAVSQTRKIDHQDEEDVAQAVRFSTWDS
                     NIQLSMAFLVNALLLIMGVAVFKTGSVQDPSFFGLYEALSDPSAMSNGILIAVAKSGI
                     LSTLFAVALLASGQNSTITGTLTGQVIMEGFIHMRIPIWLRRLVTRLLSVIPVLICVL
                     FTSTKGTIEEHTALNNLMNESQVFLAFALPFSMIPLLMMTNSQAEMGSRFKNSFVVKL
                     LGWFSVISLTYLNLRGLPGQIESFFGEQATSAQLALADQIAYVIIVLVLLLLAWTIGD
                     LYKGNKKYEQRLQETIEEIE"
BASE COUNT         8673 a         6647 c         5521 g        10159 t
ORIGIN      
        1 attgacgtca taaaaagtag ctttgaattc ataatcgttt atataacgat taatagaaaa
       61 caatatagta tttagtgttc atacaatgat tctaagatca ttttgaatta atcagtctta
      121 tagttgattt ctaaattgtc atatattatt aaaaattttt gccatttaaa aagaaaagcg
      181 acagtttacc aataaatttc aactgaatta tctaaaagta gatcagttag tttagataat
      241 tcagttgaaa ttgaccgact caaattactt gtataatacc taggaaaaaa ttaactagca
      301 ccacgggtat agttatttat atttataaca aataaaccag agaaatagcc tattttgtgc
      361 ctattctctg gtttatttac atattagaaa ttattttgtg atgatttgat ctaaacttgc
      421 tagcgacaaa gccatggcag caatttgact taattgcgtt aatcggttct tacgtactgc
      481 tggttcttct gccatgacca tcgtctgatc aaaataattt tcgatcagtg ggcgaaggct
      541 tacaagctga tcataatttt cgttgatcgt ttggtcagca aagctttttt tcattgcctc
      601 tactgctttg tgcagttctt tctccgcttc attttcgaac agttcagggt ctacttctga
      661 ttgtttttca agctccgctt ttttcgctaa gttcacgaca cgtgtcaaag cttccatcga
      721 tggtttgaag tcttcatctg caagatgctg cttgaacaat tgtgctgctg caaatacttt
      781 tgtcagatct ttttgttctg cattcaaaac agcttcgatg acatcatgac gaatattctt
      841 cgttgtcaat aattggcgca gacggccttt gataaagtcg atcacctgtt gttgcccttc
      901 tgataaacca atgccgaaac gatccgtatc tttactgata acatcactaa tcgtttcttg
      961 cagaacagac aaaggaaagg cccatttttt cgattcgatg atccgaacga caccgtatgc
     1021 ttggcgacgt aaggcataag gatcgtttga cccgcttgga atcatcccaa ctgcaaagaa
     1081 agaaaaaatg ctatctaatt tatctgcaat cgctaaaaca gaacctactg tagactgagg
     1141 tagctctcct tcactggata taggcatgta atgctcacga atcgctgttg ctacagcagg
     1201 tttctcgcct ttcagcagag catatttttc gcccatgatc ccttgtagtt caggaaattc
     1261 ccctaccatg ttggttacta gatcaaattt atagatttca gcagctcgtt gcagatccat
     1321 caattcttcc tcagatagtc ctacttgttc gccaatgata cgggcaatga cttgtacccg
     1381 ctgcattttc tcatagatac taccgatttt ttcatggaaa gtaacagttt ttagtttttc
     1441 aacgtattgg tcgattgaag ctttcttgtc ttcattataa aagaattccg catcttctaa
     1501 acgagccgtc aatacttttt gattgcctcg aaccacattt tccaatttta cccgatcacc
     1561 attacgagca gaaataaaat gaggaagcag cataccttga tcgttccgta cctcgaaata
     1621 acgttgatgt tctttcatag aagtgactag tacttcttca ggaacagaaa gatatttttc
     1681 ttcgaagtcg ccagaaaaga cagttggata ttcgactaaa ttgttgactt cttctagtaa
     1741 atcttcgtct agatcaataa cccagtgatt tttttcggct aattcatttg cttgtgtaac
     1801 gatcatttct tgtctttttt gcggtgaagc aataacgaat tgttcattca atttttcttc
     1861 atattctttt gcatgcgcaa atgtcacatc ttcgcctaag aaacgatgtc ctcgagaggc
     1921 tctgcctgtt ttgacatcta agatctcaaa cgggattact tcgtcatcta acaatgccac
     1981 gatccagtga atagggcgga tatattcaaa atcgtattct gcccaatgca tcgttactgg
     2041 gaaagtgagg cttttaatga catctgctaa acctgtcaat acttctttcg cttctttgcc
     2101 gtgagtgtat ttcgttacat agacatattc cacgcctttt aattctctaa aagtgatagc
     2161 atctgtcgtc agaccttgtc cgcggacaaa tccttcagct gctttcgtcc agtttccatc
     2221 tgcgtccaaa gcaatttttt tggctggtcc tttgacttct tcttcactgt ctgcttgacg
     2281 ttctggaatg tttgttactt tgattgctaa gcggcgcggt gtagaaaacg tttcgatcgt
     2341 atcataagtc aattggtttt catctaaaaa ttttgctgtt ttttcttcta attgtttcat
     2401 gcttggtgtg acaacatgag caggcatttc ttccagcccg atttcaagta ataagtcttt
     2461 tgccatctta ttcagcctcc ttcatgtttt ctggatttgc tgtgttttta ttcaataaag
     2521 ggaatcctaa tttttcgcgt tctgccacaa agattttggc tacagcacga gccatgttgc
     2581 ggatacggga aagataacct gctcgttctg ttacggatac tgcaccgcga gcatctaata
     2641 aattgaatgt atggctgcat ttcaaaatat aatcatacgc tggatgaaca agattttcat
     2701 cgatacagcg tttagcttct ttttcaaaac ggtcaaagtt gcttagtagc aagtcttggt
     2761 cacttacttc aaaagaatat ttggaatgtt catattctgg ttgtttgaag atctcgccat
     2821 atttcacacc atcagcccat tccagatcat agacactttc aacttcttga atatatgaag
     2881 caagacgttc tagtccataa gtgatctcag aagtaactgg tttacaaggt agtcctccga
     2941 cttgctggaa gtacgtgaat tgtgtgatct ccatcccatc aagccatact tcccaaccca
     3001 caccggcaca ccccatcgat gggttctccc agttgtcttc aacaaaacga atatcgtgtt
     3061 ctaatggatc aattcctaaa agacgcaagc tttctaaata aagctcttgg atattttcag
     3121 gtgaaggttt cattacgact tgaaattgat gatgctgata caaacggttt gggttttctc
     3181 cataacgccc gtcagctggt cggcgggatg gctctacata agcagcattc catggttccg
     3241 gtccgattgc ccgtaagaat gtatatgggc tcatcgtacc tgctcctttt tctgtgtcat
     3301 aagcttgcat cagcatgcag ccgtttgatg accaaaattt ttgtaatgtt aaaatcatat
     3361 cttggacggt caatttttta ctcatctgtt ttgccccttt caatttttaa gcgttgctgt
     3421 caataagaat acaggaaaga aaacgacaaa aagtccctat gtctgattac agacataggg
     3481 acgaataatt tcgcggttcc accctacttc cgaataaaga ttcggcagct tcatttatcg
     3541 tactcgcaag tgcctcttca aaaatcgttt gggtccggct tccactctcc cgaactcgct
     3601 aaaccacgtg ttttttactg atcttggtca atgtacgtat tcattttgca gttttcttgt
     3661 ttatataata gatcctctca gtgaacgatc tatttaatct acggttaata atagacggaa
     3721 gtccctgtat ttgtcaaata gttttgattt ttttgttgta tctttcattt attcttcttt
     3781 ttcaggtatc tctttttcag gcatattttt ctctggcgtc tttaatacgt gtgcccaaga
     3841 cttcatttga tcaatgaact ttttgctttt tagatgaata ccgacatatt catcatacaa
     3901 ctggtcaatg aggtcacgga ttgcctgctt cgtttcttct ttcaagtgga ttccactgat
     3961 cttatcataa gaaatagccg aaaacattcg aacgaaataa acagcgcgag gatctgcatg
     4021 ataacggtgt tcgtccattg gccagtgttt ctcgcatatc acaccgccag atttggaaga
     4081 atagtcaaat tttccttttg tttccccgca tacgacacag tgcgtccaaa ttggcgcgat
     4141 tccgaagcgt tgcagtattt gaacttcaaa gatattcgtg atgatttctg catcatctcc
     4201 ttgatccatc cgctgcagag cttgttgcgt aaattggaat aaatgcgggt catattgatg
     4261 atcttcgatt gctgcatcta ctagatttag caaataggta ccataaccgc tcaaaaagat
     4321 atcttcttgg atgttccgaa acgcatggac gtctttgaca ctgcttaaaa acgacaaacc
     4381 ttcacttcga aaattaccga tatagactgc ttcggtaaag gggagcaaag ccggagctaa
     4441 tggctgattt tgccgatgga ttcctttgat ataaaacatc attttgccag aagattccgt
     4501 aaagattttg actaatttat ctttttcttt gtaattacgg ctagataaaa tcaagccttt
     4561 cgattctgca agctggctca tattctccct cctaaaaaat aaatctggaa aagcagattc
     4621 tgccgatcca gattatttat gtttcgatct tccgccgttt ttctcaacgt gcggattttt
     4681 atccagttta tttctatact taacctataa gaagttcaga atatcagtat tcgtcttttc
     4741 ggtagccaaa gtcttgcaga tagacttttt tgtctcgcca gtctttttgg actttgaccc
     4801 aaagttctag aaatactttg tctcctaaaa gattttcgat atcctttctt gctttcgttc
     4861 ctacttgctt cagcattttt ccgcctttgc cgataatgat tcctttttgg ctatctcttt
     4921 cgacgatgat cgtggcctgg atatggattt tatcattctc gtttcgcttc atcgaatcaa
     4981 caacgactgc gacagaatga ggcacttcgt ctcgtgttaa aaacaagact ttctcacgga
     5041 tcaattcaga aacgataaag tattcaggat gatcagtcac ttgatcatct ggaaaatatt
     5101 gtgggccctc tggcatttca ctgactaata catccattaa gcgttcgaca ttgtttcctt
     5161 ctgtagcaga gatcggaatc acttgtgcaa aaggcatctg cgtcgaataa tcttcgatga
     5221 ttcccagcaa ctcgtctgga tggatcttat cgattttatt gataatcaga taaactggag
     5281 aatgcacgtt ttttagacgt tcaataataa aatcgtctcc ccggccgcgt ttttgatcag
     5341 cactgatcat aaataagact gcatccactt ctttcaacgc actgtaagct gcttctacca
     5401 taaaatctcc tagacgatgc tttggtttat ggattcctgg tgtatcgata aaaatcagct
     5461 gagcttctgg tgtagtatat actccttgga ttttatttct agttgtctgt gccttgtcac
     5521 tcataatggc aattttttga cccacgattc ggttcaataa tgtggatttt cctacattcg
     5581 ggcggccgac gattgcgaca aaccctgatt tatgttctgg catatacgtt ctccttttta
     5641 attaaacaac aaatgatata ttttagggac aaataaaatc aacccaatga tgacagcaaa
     5701 acaagcggtc actaaaactg ctccagctgc catatctttg atttttttcc caatcggatg
     5761 aaaatgaaaa tccgtaaaca tgtccactac attttcaaaa acagtattga tgatctctac
     5821 tatccaaacg agaaaaacag ctaaaagcag ccataaccac tctatccgat caagctggaa
     5881 aatcacgccc ataaataaag caaggatacc gaacgcaaca tgtttcctca tattgcgttc
     5941 ttcctcaaaa acggttttga ctccttggac agcaaattcc acagacatca ggaaatgttt
     6001 gtttttctct acgtttttat cttttgagtc cataggcatc caagatctct ttctgtaaac
     6061 caaacatttc tttctcatct tctggtgtca tatggtcata cccgttgata tgcaagaatc
     6121 catgtaccgc taaaaagcca agttcccgat catatgaatg gccgtaagct tctgcttgtt
     6181 cagcagcacg ttcaatagaa atcatgatgt ctcctagtac acgaggcaaa ggattctctc
     6241 catcttcaaa aatcactggg atttcgtctt cgccttcttc ttccagagca aaactgatga
     6301 cgtctgtcgg tgcatctttt ccacggtaat cacgattgat tacttggatt gccgcattgt
     6361 ccatgaaagt aacagacatt tctgtttcct ctggcagctt taaataagat gctgcaaatt
     6421 gcagcaagtc atcgatttct ttcattttct cttctgagac ttggtttgtt tcatccataa
     6481 aggtaatatc cattatacat cgcttctttc tattcttctt gttcttcctt tatcttctca
     6541 acttctgttt tcattttagg atatttgatc cgagaatgga aggtgctact taaaccgctg
     6601 atcaatgatt tttccacttt tcggatttct ttcaaggtca gtccactatc gtctaattga
     6661 ccatcggaaa tacgctcttc gataagatta tggacaaaac tttcgatttt ttcgctcgtc
     6721 ggatgatcca ttgcccgtac tgcagcctca caactgtcag caatattgac gatccctgct
     6781 tcacgacttt gcggttttgg tccaggataa cggaattgct cttctgttac ttccggattg
     6841 cgttctttcg ctttgatgta gaaataacgc attagcgtgg ttccatgatg ctgtttgcaa
     6901 atatcgatca ccatttgcgg catattataa tcttccaata ttttggctcc atctgtcaca
     6961 tgtccaaaga taatatgttt actgtcttct ggcaataaga aattgtgcgg gttttccgct
     7021 cctgaaggca agttctcaac gaaaaagcta gcatgtttga tcttgccgat atcatgataa
     7081 tagcaagcta ctcgagttag caaagaacgt ccgccgattt ctgctacagc gttagcactt
     7141 agattagcta ccatcatact atgatgataa gtacctggcg cttcttccag caactgtttc
     7201 aataaaggat gatttggatt gctgagttca tttaatacaa ttacactgtc atcattgacc
     7261 atcaattcaa tatatgggtg tagaccgatc gtcagcaaga atgagaagag tgcaccagtg
     7321 aacccgcaga caagcattaa ccaagtcgtt gcatcaccaa agctcattcc ttgatagacg
     7381 atcaaaatga catccatcat cacaggaaaa ataatgaccc acatggcagc agataatcct
     7441 tgttctgaga tccgtttgcg tctgacaatc gttgccatga gcccagagaa aacataagac
     7501 atcaagataa tcgttagaaa gttcgtgcca atcgaatcat aaaaaatgaa aagtgcagat
     7561 actgcttgga aaataccagc catgatccct gctcttcggt tcaagaaaaa gcttaaaaca
     7621 agtggtgcaa aagcagcggg ataaaacagc gggatataag tagcttgttc cgtttggaat
     7681 aattggaaaa atttcatcag cactatgctg actgacatag ctgtcacata gaaaagcacg
     7741 tattttgttc tttctcctgc attttggaat tgaagtgaat tataaatcag tacgccgatt
     7801 tgaagcaaaa cagctaaaac catggcaatg agtgggaaaa tcgaagtcgt ttgattcgtc
     7861 aatcctagta attccaattt tttcatcgct gaagcatcaa tttgatttcc ttcgcgaaca
     7921 atgatttctc cttggaagat catcactggt tgtacagctt ctcgtgcaga ttgttttaat
     7981 tcttctgtct ttttctcatt caatacatca ttgactacaa tgccttgatc cactaagtag
     8041 cgaatcattt gttgctgctc tgttgtgaca ttcaaatact gaatctgatc ttctgcttct
     8101 tgtttaaaag cttccaattc gctttcacga acttgtttac tcatctgttc gtcaatgagc
     8161 ttcaaacttt catccctaac cgtgtgaatt tccgtttcag tcatttcaaa tatcgtcgaa
     8221 taaaaattgt tcggcagttt ttggtaaaac gcaacgtttt ctgcattcac gttttcaaaa
     8281 tttttcttca gtgtagcaat ccgctcttct gtagtaggtt tcgggatatt ttcatcttcc
     8341 ttcgccttat ctaccttgtc attataagat ttgttgatgg tatcattcgt cttatcgatc
     8401 aattcgaata attgcttgat ccgattgttc tggtcatctg ccagatcttt ttgataagta
     8461 tattctggag tcactgcttc tgctgccagt tttcttcttt gttcggtttc ttccgtgttc
     8521 tcgatcgttt tattcgcacg gatactctct tctgctactt ggccttctct gaaatcattg
     8581 gccttttgtt tgacactact aaatgtaacg ccgcagacaa tgataaaaaa caagaacaga
     8641 aggaaaggca atgctgcctt tccgagtttt tcttgtagtt tctgcagtgt tttattcaac
     8701 atgtgcttat cctcctaagc aaagcttatt cttgatgaag atccgccttt tcataagctt
     8761 tgatgatttc ggcaactaca gggtgccgaa caacgtcact tgcttcaaaa ttaacaaaat
     8821 cgattttttt gatttgcttt agtgttcgtt ctgcatgaac aagaccactt gtcgtccctc
     8881 gaggcaagtc tatctgactt gtatccccat tgacaatcat ttttgattga taaccgagac
     8941 gggtcaagaa cattttcatc tgagcgactg tcgtgttttg tgcttcgtcc aagataacga
     9001 atgcatcatc caatgtccgc ccccgcatgt aagctaatgg tgcgatttcg attacgcctc
     9061 gttccatcaa tcggttcgta tgatccaatc caaaaatttg gtataaagca tcataaacag
     9121 gccgcagata aggatccact ttttctttca gatccccggg tagaaagcct aggttttccc
     9181 ctgcctctac agcgggccgc gtcaaaatga ttttttgcac ttcgcctttt tttagggcag
     9241 caattgctaa tactacggca agaaacgttt tccctgttcc ggctggccct acaccaaata
     9301 cgatgtcatg tttggctacc gattcaacgt atttcttttg accgctgttt tttacccgta
     9361 taggttttcc attacggtct ttgacgatct cttcctcata catctcaaca aaataatcta
     9421 atgtaccttt ttgccccatt ttcaatgccg tcacgacatc tggtgtactt acgtggattc
     9481 ctcgtttgat caattcctgt aatgtccgaa tgactgaggc agccaaggaa atctgggatt
     9541 gttcaccaat gatctgaatg atttctccac gtgtatggat ggttgtttcc gttgtttcct
     9601 cgatcagttt tatatgcttg tcgtgtgcac ctaaaagcat gctgacatca tcagcagctg
     9661 tcaatctaat ctctaaagaa ctagactctt ccgtcaaaaa tagacatccc ctttttttcg
     9721 attcattctt tcctatttta ccatactcag aagaaactat atagcaaata agaaaaggtt
     9781 gtaaaagagt cgtttaacca tacgtaccaa gaaacgttaa aaaatagctg tttccattcg
     9841 ctaaaggtct cgttttgata aaaagaatct gagaatgtaa gttatatgaa agtggagaac
     9901 aaaatattcc gtttcattca gaggtattct ctaggtattt ttcttgtttc aaaaaaagaa
     9961 aacaaaatag ttaaaatatt cttgcatttc aaaaaaaaca ggtgtattat tacgtcaaca
    10021 aataaacata cagcaaagcg tcgaggaaaa cagtaggcta ttcaacgatt tattcccagt
    10081 gagtcatgta agtgagaaat gacaataaat cgaatagacg aacatcattt ccgagtcaat
    10141 gtcgaaaacg tagatcattg tggctgcctc cattatcagg ctagcggata actttatcaa
    10201 aaagcgtccg tgaatgaaga agctttttta agcgaaaaaa ggtggtaccg ttctagaatc
    10261 tagagccctt ttcccaagtg gaaaagggct tttttttata ttttaaggtg gtggttttca
    10321 tggaatctga tattagtggc attactaatt gctgcaacgt ttatatcgtc aatcaaaatg
    10381 ataattattg gaggaaaaat ttatggaaga aacaaaaact tattcatgga aacaactatt
    10441 gatggtcagt tcacttatct tcgggatgtt tttcgggtca ggtaatctaa tctttcctgt
    10501 ccacttaggt caaatggctg gcgcaaactg ggtcagtgct ggcgtaggat ttgccatctc
    10561 aggcgctctt ttcccattac tagctatctt agctgtagta gtgacaaaaa gtgatggtct
    10621 gtatgactta gctaaacctg tcggaaaatg gtatgctgcc ttgttcttaa tccttgttca
    10681 tttgacgatc ggaccatttt tcggtacacc acggacagct gctactgctt ttgaaatggc
    10741 tgctaaacca tttttgcctg aaaaatataa ttcattaggt atgtttttat tctctgctct
    10801 tttcttttta cttgcttatt tccttactgt ccatcccaca cggttggtca aatacgtcgg
    10861 aaagtatcta aactccttgt ttcttgcatt attagcaatc atttttgtcg tttctttatt
    10921 ccacccaatg ggaaatctaa atcaatctgc tgcagatact taccaaacaa acgctttaac
    10981 aaatggtgtc ctagaaggtt ataacacggt tgatgcagta gccttgcttg ccttaagtgt
    11041 cacttttgtc catgcagtcc gtgaccttgg tttcaaagat aaaaaagtca cagaattaac
    11101 agcaaaagct ggtagcctga gcgttgcgct agaagtcatg atctattttg gtcttgttct
    11161 tctcggtgcg atgagtttga ataagatgtc tttatcagaa aatggcggaa ctgcactttc
    11221 tcagatcact aatttttatt taggtaactt cggcgctagc ttcttaggag tgatggtcac
    11281 attaggtgtc ttcactaccg ctatgggact cgttgtttct tttgcacaag atttccataa
    11341 actgttccct aaagtcagtt atatgacatg gctgcggtta acgactttcg tctcttttgt
    11401 cgttgcaaat gctggtttgg acaatatcat ccaatggtct ttaccagtat tgatgctttt
    11461 gtatccattg tcgcttgcgt tgattttgct ttccttaaca gcaaaatttt tccaaaagac
    11521 accatttgtc tatcaagtaa cgatgttgtt tgctgctgtt cccgccgtat tggatatgct
    11581 ggcaaattcg cctgctttag tgagtcaaca aagagttgta gcaagcatgc tcgaatttta
    11641 tcatcatcac gttccttttg ctgcattggg acttggctgg atggtcccaa cgttacttgg
    11701 gtatgctggc agcttactct tctattacgc ctatcgccta tcagggtata aacaagaagc
    11761 aaatgaacta ccagaagaat aaccagatca aacacaattt aacataagaa atattaaaca
    11821 ggaaaaaaac agctagatga aagaatccta ttctttcgtc tagctatttt ttagatctct
    11881 tttttacgag cacaaaaaaa ctgcggacaa tgattgtccg cagtttaaat gataaaatta
    11941 agattgtaat agttctttca caatggcatt tacttggttt ccatctgctt ttcctttgac
    12001 tttaggcatg atcgcaccca tgactttgcc aaattctttc attgatgaag caccagtttg
    12061 cgtgattgct tcttgaacaa gttgtcgaat ttcttcgtcg gaaagttgtt ccggcatgta
    12121 tttttcgact atcacaattt cactttttac tttttcagct aaatcgtcac gtcctgcttc
    12181 ttcaaactca tgcaaagaat cacgtcgctg tttcatttcg cgagacaaca ccgttaattc
    12241 ttcttcttct gtcaagtcgt gaccttcttt aatctgctcg ttttgtatag atgacttgat
    12301 cattcggatg acttgtaaag attccttatc tttcgctttc atcgctgttt tcatgtcatc
    12361 gtttaatcta cttagtagtg acacactacc aactccaatt catcacagaa ctagaatttt
    12421 ttgcgttttc tagctgcttc agattttttc ttgcgtctta cacttggttt ttcgtagaat
    12481 tcacgtttac gagattcttg taaagtaccc gcttttgaca cggaacgttt gaagcgacga
    12541 agagcatcgt caagtgattc gtttttgcga acgactgttt ttgacatgtt aattccctcc
    12601 ctccgagctt aaaaagtaac cgtaattgtt attgaaatat atctcaaaaa acaagactgt
    12661 cctgtaaata ttatatctaa tgctgttttg accgtcaagg aaatcttgct tttttttaaa
    12721 attatcaaat gttattttat ttaaatcctt attgacaatt ttcacatctt ccgaaaatct
    12781 cgaatctatg cccttcgatc gtacatccac cgagctgttc ttcgaaataa ttcattggac
    12841 acatatggat ttctttcgtt gttccgcaca ccgtacagat aaagtgatgg tgatggccga
    12901 tttccccttg acagcaatga aaacgaaact tcatctctcc atttaaatcg gtttcttcca
    12961 gtaaatcgat ttctgcaaaa tcatggagat ttcgataaat cgtatcgtaa cttactcctt
    13021 tgaattcctg attcatatag tcaaaaactt ctttagcgga tacgtagcga ttgcgcttca
    13081 ctaaataagt taacaatgct tctctttttt tagtatattt aaaaccgtta tttttcaatg
    13141 tttctagtga ttgttcgatc aacgtctgtt ttttcatgac ttattcctcc aaatcaaaat
    13201 gattacgaag tacacattct agagtatggt ataataaaca taaaaaaaat gcaagtgagg
    13261 ttttatccat gtcttccgat tctacagaaa tcgttacttt ttttgtcatc tccgactctg
    13321 ctggagaaac tgcgacaaaa ctagcgcaag ccactatggc ccaatatcct agcgttgagt
    13381 tcaatttatt ccgcagaaca tttgtgacag atgagaaaac attgatgcag gcactgcaag
    13441 atgcactaga agaaaaagct cttgtcctgc acacattgat caatcaagaa ttgatcgaga
    13501 tgacgcgttc tttttgtgaa gaacatcaat tatttagttt tgatgtgatg actccgccga
    13561 tatcagaaat cgagcgctta actggtatca agcccattcg gcaacctggc gcccttcatt
    13621 tattgaatga gaactatttc aaacggatca aagcaatgga atttgctgta aaatacgatg
    13681 acgggaaaga tcctcgtggg tttctagaag cagatgtcgt tttgctaggc gtatcccgaa
    13741 cttccaaaac gcctttgagc ttgtttttag ctaacaaaaa tttaaaagtt gccaatctac
    13801 ctttgatccc acaagcacat attcccaaac agctttggga gatcgatcct aaaaaaatcg
    13861 tgggactgac gaataaccca gatattttaa acaatatccg taaagaacgg atgcgctctt
    13921 acggattaaa tcctgatact gcttattccg atatcgaaaa aatacgagct gaactcgatt
    13981 ttgcgaatga tctttatgaa aaattgggct gcgtagtaat caatgtcgct tctttatcta
    14041 ttgaagaaac agcttcgctt attttgaatg ctttagacct agaagaccat agttattatg
    14101 gaactgaaac caccgaagaa acagactaac aatttttctt taaaaaaact ggactgtgtc
    14161 agcgaagaag attcgttgac acagtccagt ttgtctctat ttgcacctat cgttattgat
    14221 gttccacatg tttgagtgtc tggttcaaaa tatccagtac gtgagaatca tcaagactat
    14281 acaaaatcgc ttttccctca cgatgggact tgacgacatg gttttctctt aataaacgca
    14341 gttgatgcga tacagcagat tgttccattt ccactgcttg gatgattgct gtcacatttt
    14401 gttccccgtc tttcagatac agcagaattt tgagacgagt ggggtcactc aacattttga
    14461 atagttggct gactttctct acttttgttt tttgcgtgtc tggcaatcca ttcacctcca
    14521 acttgaaaaa agactgactt ctaaagttta ccactttttc catcagtctc aagttttttt
    14581 attcttctgt catcttttgg ataatcgttt cataagcggc tactttttca gctgcgtctt
    14641 ctgaagagtt tccttttaca ccgatataaa atttgatttt cggctcagtt cctgaaggac
    14701 ggatagcgat ccagccattt tcttccagcg tatacttcaa tacgtcagat ggcggtgtcg
    14761 ttagtttttc tacgtttcct tcacggtctg ttctcgtcaa ctctttgaag tcttctgttt
    14821 ggatgacttt tgctccggca aattctgttg gtgcttcttc acggaatttt tccatcaaag
    14881 cagagatttt cgcagaacct tcttgtccgc tcattgtcac agaaatcgtt ttttctgcgt
    14941 agtagccata ggtttcaaaa atcgtttgca agccatcata caacgttttg ccttgttttt
    15001 tatagtaagc tgctacttct gctaataaga gcaatgcttg gatcgcgtct ttgtcgcgaa
    15061 cgaaaggttt aactaaataa ccgtaacttt cttcgaatcc gaacataaaa gtatgggaat
    15121 gatcttcttc aaattgttga attttttcag caatgaattt gaatcctgtc aatacattga
    15181 acatggtagc cccatagtgt tccgcaattg ctgtaggtaa ttcgctagaa acgatcgatt
    15241 tcaataccgc tgcgttttca ggtaaagtgc ctcgcgattg gtgtgcttcc aaaatatatt
    15301 gagcaaacag tgctccgatt tggtttccgg tcaatacttc ataggagcca tttggcaaac
    15361 gaacggctgc acctaaacgg tctgcatcag gatctgttgc aatcagcaaa tcagcgcctt
    15421 ctttctcgcc aagtcgtatc gcatactcaa aagcagagtg ttcttctgga tttggtgatt
    15481 tgactgtagt gaaatctggg tctgcgacag cttgttctgg aactagtacg aattgttcga
    15541 atccagcttg tttcaacgca cgttcgccca acatttttcc tgttccgtga agtggtgtgt
    15601 acacaagttt caaatctttc cccattgttt ccaccaattc atgatcaatc gttactgctt
    15661 tgatttcttc tagataagcg gcatccactt cttctccgat gatcgtgatc aatccgctat
    15721 gttcagcttc ttcgtcagat aatacttcaa cttttaacgg attgctgact tcacggacat
    15781 atttcgtcaa tgcatctgcg tctgcaggag gcatttgtcc cccatcttct ccgtagactt
    15841 tatatccgtt gtaagctgct ggattgtggg atgcggttac catgattccc gcaaacgtct
    15901 tcaaatgacg gactgcaaaa gaaagctctg gtgttggacg tagactttcg aatacataag
    15961 atgggatatt gtgatgtgct aatgttttgg ctgcttccat ggcaaattct ggtgattgat
    16021 ggcgtgagtc ataagcgata gcgacacctc ggcgttttgt ttcttcgcct tgtgtatcca
    16081 tgaaacgtgc aagtccttct gttgcttgtc ttaccgtata gatgttcatt cgattgatcc
    16141 caggccctaa gattccccgc atgcctgcag taccgaattc caacggtgca taaaaagcat
    16201 cttcaagttt ttcaggatct tcacccagat catttaattg tttttttaag ttttcttcta
    16261 aattttcttc atgtacccat tgttcataca cttgttccca agacatgaaa ataacacctc
    16321 tttctgaaat atggttctta ataagtatag cattcactta ttagatatga aagtatgagg
    16381 accaaaaaac gcttaaatca agaaaggatt cttttatcaa gcacggttgc aagtattcgc
    16441 ataaatcgga ttcagtagat ttcacgcgaa aaaaaagagt aaaagaacaa ttaaaccgtt
    16501 cttccactct ttttaaactt atgcagttgt ttcttctaaa gattccaagt atttatacac
    16561 ttgttgtcca gcgatcccac catcaccaac agcagtagtg atttgtcgca aatctttttc
    16621 acgaacatct ccgatggcaa aaacgcctgg tatattcgtc ttcatttcgt tgtctgttgg
    16681 aatccagcca gcttcgttcg tcaaccctgc ttctctaaat ggtgcagtta atggatccag
    16741 tccaacatag atgaatactc cgtctgctgc caaatcaaac tcattgccgg ttttgacatt
    16801 acgtgctttc acacctgtca ctaccatttc attcccaacg atttcgtcaa cgactgtatc
    16861 ccacataaag gaaatttttt cattcgcaaa tgcacggtct tggatgatct tttgtgcacg
    16921 taattcatct ctacggtgga cgatgactac ttcagaagca aattgtgtca aatagatggc
    16981 ttcttctact gctgagtctc ctcctccgat cactactaga cgtttgttac ggaaaaacgc
    17041 accatcacaa actgcacagt aagaaacgcc acgtccagca aattcttcct cgccaggtac
    17101 gcctaattta cggtggatac agcctgttgc gataatgact gtttttcctt cgtaagtttt
    17161 atcttcacaa ataacttctt tgtggctgcc gtgatccttg atatcttgca cgattccata
    17221 agcattttct gtcccaaatc ttgaaacacc ttcatacatc ttataagcta gatcaggacc
    17281 cattattgaa tcgaaaccag gatagttttc gatttctgcc gtattgttca tttggccgcc
    17341 aggggcgcct tgttcgatca tcaatacgga taagtttgaa cgtgaagcat ataaagcagc
    17401 agtcatccca gcaggacctg cgccaataac gattacatca tacatatgta gattctcctt
    17461 tacttaatca agtaagcata ctttacagcc aatgcgaaaa aaagtctatt gttctgcttg
    17521 cgtttctcga atagctttat tatactcgaa aaatccgctg aaaaacaata gacttttata
    17581 aatcattgat caatcattcg aaagattttc tccatttgaa tcaattactt ttttatacca
    17641 gttgaatgat ttcttcttcg aacgggctaa tgtgccattt ccttcgttgt cacgatcgac
    17701 ataaatgaat ccgtaccttt tcttcatctc acctgtacca gctgatacta aatcaataca
    17761 tccccaagtc gtatagccta atagatcgac tccatctaat tctactgcat ctttcattgc
    17821 ctggatatgt gctgccagat agtcgattcg atagtcatct tccacataac cattctcatc
    17881 gggcgtatcg actgcaccta agccgttttc tacaataaat agtggttttt gataacgatc
    17941 ataaatatca ttcatcgtta ttctcaagcc taatggatca atttgccaac cccattcact
    18001 tgcttccaga tatggatttt tgacagaagc aaagatattt cctgcagtct gttccaagag
    18061 ttcgttgtca gtagtcgaaa ctcgagaaga ataataagaa aaggagataa aatcaacagt
    18121 atgttctttc agcaatgcgg catccccagg ttccattttg atctgaatgc catttcgttc
    18181 catttctttc aatgcataag caggatattc tcctcgtgac tggacatcaa tgaagaaata
    18241 attttctcga tctgattttc tagaagccca aacatcttca ggattgcaag tgtatggata
    18301 atatgctcct gctgccagca tacagcctac tttgttctcc ggatctactt catgagcaat
    18361 cttcgttgcg atggcacttg caaccaactc atgatgtgcc gcttgatatt tgatttgttc
    18421 ttcattttct ccttcttcaa aatacagccc tgcgcccatg aatggtgcat gaaggatcat
    18481 attgatctca ttgaatgtca accaatattt gacaagtcct ttatagcgat tgaagatgac
    18541 tcggcacaga ttttcataaa aaccaacgag ttctctggaa cgccacgcac catattcttt
    18601 gatcaaatgc atcggacaat caaaatgtgt aatcgtcaca agcggctcga tcccgtactt
    18661 ttgacattct ttaaagacat cctcataaaa tttcaaacca gattcatttg gctctttttc
    18721 atcgcccatt ggaaaaatac gactccaagc aatggacatc cgataagtct tgaaccccat
    18781 ttctgcaaat aaagcaatat cttccttata atgatgatac atatcgatcg caatttttgc
    18841 tggataaaaa tgattttcat caaattcaaa cattttcttt tttccagtaa tgacaggaaa
    18901 acggtcttct ccgattggca caacatctac gttcgctaac ccacgtccgc cttcatcata
    18961 accgccctcg cattgattgg ctgctgtagc tccgccccat aaaaaatctt ttctgaatcc
    19021 catcatactt cctccattac atgtgtattg cttgataatt ctcttgccag ttcatcttgg
    19081 acctcgattc cttctgcttt taaccggctg attgtttcct tgaattgcgg taaatactgc
    19141 tgatgtgcaa taaacaactc attcatgatc cgttttgctg tctttcctga tcgtataagc
    19201 ggattgatcg taaacgcttg taaagctgtt ccataatcac cagtcacagc tgcttcgatc
    19261 gtcagcaatt ccatattctt cattacttgc aaccagcctc tttcagcagc aggaagggaa
    19321 ccaaacgcta catttcgtgc gccttgtgcg ccaacatagg cagtgacttc tactacacaa
    19381 tctgccggca gatcaggaac agctccatca ttctttgtag ataccacgat ctgtgtttct
    19441 ttatttccat agatcgaagc aatcgtttca caggcagcat ctgaataata agcaccacct
    19501 cgtttttgca attgttcagg tttatgatcc aattctggat ctttgtataa ttcaaacagc
    19561 tctgcttctg tttctttcac ttgctgcgct cttgtaccaa tcgtttgata ttcttctaaa
    19621 gcatgttcca gcatttcttc ttcacggtag taatagcggt ggtagccaca agggatcatg
    19681 ttcatttgtt ttaattgttc tttgaaaaac gggatatcaa agatattttt cggcaatcca
    19741 ttgtcttcat cgtataaacg atcaatcaat tctgccgtgc ggtcatttcc tttattatct
    19801 gctactttgt gccaatggaa gtgatttaga ccggcaaact tgtagatcag ctcttctttt
    19861 ttcttgccaa gcattgttgg ttctgtcatc atcgccatta ctggcacatt gcataatcct
    19921 atcactttat cccatttgcc atatctgata actgcttctg tcaccatccc gcttgggttc
    19981 gcgaaattga tcagccaagc atttggacat aaacgcttca tgtcttcaac gattgccaaa
    20041 accactggaa cggtacgaaa agctttgaat atcccgccag caccatttgt ttcttgccca
    20101 atcatcccat aataactagg gatccgctcg tctttgattc gtgcatctaa ttgaccgaca
    20161 cgaaactgag tcgtaacaaa atccgcatct ttcaatgctt cttcccgatc cagagttgca
    20221 tagattttga cttcatacgg agaagcatcc cacatacgct gagacatctg actgacgatc
    20281 tcaagctttt cttttccttc ttctacatct acgagccaga tttctcgtat cggtaattcc
    20341 tcatatcttt tgataaatcc ttccatcaac tcgggggtgt aactgctgcc tccaccaatc
    20401 gtagcgattt tgattccttt tgccatgttc tcactccttt actttttaca cttttattat
    20461 cttttttgta aacaaaacaa acaatagaaa ttcgtttctt tccagtaaca tttacaaaag
    20521 ttagtgtttt ctactggatt tttgtaaata tcttgtcaaa aaatgtcttt attaagctat
    20581 ataacataaa tccgtttaca aatcattttc aaaaaagtta taatttacgt aaaaaaacaa
    20641 gaagaaggta tctacatgtt attacgtgaa aaaatcaaag aaatctcctt ttctccttca
    20701 gaaaatgaag tgattcagtt tctcttaagc gattctccag cacttggcga aatgaccatc
    20761 caagagattg ctgagcgatg ttatgtccac ccgtccacac tcatccgtgt agcaaaaaaa
    20821 atgggttttg atggctgggt cagttttcgt aatgcttttt tagctgaaac agcctatctt
    20881 gaagggaatt ttcaagacgt agatgcgaat cttccttttt ccaccacaga cggtattatg
    20941 acgatcgcaa ataaaatggc ttctttagaa cagatgacga ttcaagatac actttctctc
    21001 attcatcatg accaactgca aaaagccaaa caactaatgc ttcaagcaaa aaaaatcgtg
    21061 attttcgcca gtaactcgaa tactttgatt tctcaagatt tcatgctgaa aatgaagcgc
    21121 atcaaaatgg atgtcacgat tgtcaccact tttggtgaat cgttctatga agcgtacaat
    21181 tgcacgagtg atatgtgcgg gattctgatt tcttatacgg gtgaaaacaa tgtgatcctt
    21241 cgtaccgcag aaatattgaa acaaaatcat gttccaattt tagctttgac cagtatcggt
    21301 gattcaacgc tatcttcaat gagtacagcc gctttgcgac ttacgacacg cgaacgactg
    21361 tattcgaaaa ttggtaattt tacgatcaat acatcgattt gttatcttct agacgtgtta
    21421 tatagttgtg tttttgcaga aaattatcaa cagaatttag tccatctaat ttctgtagga
    21481 caaaaatttg ataaacgccc tatcagctca gccatcatga aagagccttt caattttgaa
    21541 aataaagaag tggactaaaa agaataatta agcctagtcc attctttcac ctaaccgttc
    21601 aggtcagtgc ggtgataagt gcatcgtctg gcccgaccgt ctccccttga ttttccaaaa
    21661 tatccaaata atccgtgtaa ttggtgacga tcacaggtgt ttctaaactg tatccttcct
    21721 cttcgatttt ctttttgtcg aagcggagca acagatcgcc ttgtttcact ctttctcctt
    21781 gctgcaccaa gctttcaaaa tatttccctt ccaactgaac agtatccaat ccgacatgaa
    21841 tcagcaattc cagacctgct tcagaaacca ttccgattgc atgtttcgtc gggaacaggg
    21901 tcatgactgt tccgtcaaaa ggggcacgta cttctccttt ttcagggtag atcaagattc
    21961 ctcgacctag tgttccttgt gcaaaagctg catcctttgc tgcattcagt ggagcgattt
    22021 gtccttgtat cggtgttttt acaatttctt tacctgatct tttctgtcta tcatctgttt
    22081 cacctgcttc ctccttccaa aagaaaaaag tcagcgcaaa ggataccacc atcgcaatgg
    22141 cgattccaat caatacatta atcacatccg ttaaatcacc atctggtcta atatagttgg
    22201 gaattgtaaa gatgcctaat ccacctggat tccaagaagt taaattcatt aatccactat
    22261 acgcgccccc taacgcacca ccgacacagg taaaaataaa tattttttta cgtggcaatg
    22321 taaacccata aatagctggc tctgtaatcc ccacaatacc agaaacaatg gctggtattg
    22381 cgattgcttt gtcttttggg tttctaagct taaagtagag tgcagtaagt gctgcagttt
    22441 gtccaaaaga cactactgca acaggtgtca tgatattttg ccaaccattc acagaaaatt
    22501 gcatcaatcc cattggtacg attgcccaat ggagaccaaa catcactaga atttgccaga
    22561 agaagcctac gattgctcca taaataactg gcgaaagtgc taaaagtgtt tcaaatccag
    22621 ccattaaaat atcagttgcg aatgtgaatg ctggtccaac gagtaaaaaa cctaagggca
    22681 ttgtaataag aacggtgaag aatggaacga agaaattcgc gatcatctca ggtactaact
    22741 ttttaattgg tttctgaatt tggctagcta acaacacaat aaagataatg ggaatgacag
    22801 aagaagcgta gttacgcgca atccagggaa tgccgaatac tgtttgataa ataggtgctt
    22861 caaaaatagt gccagaaaat atggtagtca gtggtgcttc tgctgcacca ctcaaagctt
    22921 ccagcgacaa gctaggattc attaatgctg ccccaagcaa catccctacg aattcattta
    22981 ctttaaattt acgggcagct gttaaaccga taataacagg cataaattgg aatacagaat
    23041 caccgatatt atcaaacata acaaatgtgc cagaaccgcg gtcaaatgcg cctaagaaga
    23101 caagtaatgc tgtcagtcca cgaatcatcc caccagcagc tagaatagca aggaatggct
    23161 ggaaacagcc cgatattcca tcaataagca tatcaaacgg tctcattttt tctgttgaaa
    23221 cagcctcatc aaaagtaagg tttcctgctt ttataacctc ttcaaacacg actggcacat
    23281 gattcccgat gacaacttgg tactgtcctc ccgctcgcat taccgtcaca acaccgtcca
    23341 tatttttcaa gatctcatca tttgcttttt cttcgttttt taatttgaaa cgaagccttg
    23401 tgatacaatg tgtcaaacca ttgatatttt ccttcccgcc gacattttct acaattttgc
    23461 tcgctaattc ttgatatttt cccatttcta ttccctccaa tttattagtg gaaggtttag
    23521 ccaactgaat gtcactaccc ttatttcatg cgttcttgtt aatttatcct ccttttttta
    23581 tttatttaca acgattctca tgcactaaac gcgcaatatg gatcgttaaa tacatttttt
    23641 cgtccgtgga catctggtag ttgaatatct cggctaaaaa gtctgctatt ttctcaacgc
    23701 aaccaaacgt atccttatat tttttttgaa taacctggta caattcctca gaaacttctc
    23761 ccacttctga tttttctcca tctaggacgc gtgtcagaaa aaaacgtagg tgtgtgacga
    23821 atcgaaaaaa atggactgaa tcttcatcaa atgtaatctt atagtaatag cggacgatat
    23881 ttagaatatc ctgtattgtt gtagtaaaca ggtaggtatt gtcttgttgg ctttgaatgc
    23941 cttgtgcatt gactaaatgc aatgcgataa atccggcttc atcttctgat aacttgatgt
    24001 ggtatttatc cgagattttc ttcactgttt ttttcccgat ggcatactct ttaggaaata
    24061 gttgtttgat atcatacagc aatacatttg gtacaaatat tcccattttg tgacgttcga
    24121 tagccgtata caaatgatcc gtcaatgcaa tatacaccga atcattcaac tggacttgta
    24181 gagcctcttt tgtatattca atgatttcac aagaaatttc taagtactca agaggaatcg
    24241 cttgtgccaa ttcttggaac tgttgtgaaa gtccgtcatt tgtcaactga aattttttgt
    24301 cgatgcatga gtcaaaaatc gggtcaccca ctttcttttt gaatgccaaa ccttttccca
    24361 tcacgatcat ttcctgattt ctttcatccc tggttattac gacgttgtta ttcagtactt
    24421 tctcaatcag catgttttac tcctttccat gatatatagg gctgtatcca aaacaaaaag
    24481 aaaaacccaa aaagccgtcc atggaaccat gggagactca tcaggtttag cttgcatcag
    24541 gcaatcacta tccaagttgt atcgttttct ttatattatc gaaagcaagc acttttgtca
    24601 acgctttctt agcggctatt gggcacttct gactagctgt cgtgattttt tttaaatctg
    24661 gtaattacga cactaacgct cacagatgaa gaatctggga agctctgttt tattttcact
    24721 ctagcgctta ggtcaaaatg ctactatgct actgtcccaa cttctaatat acggtgttcc
    24781 agtaccagct tatcggtgat aagccaaatt tatgcaaaaa tctggaaagc cttttttaca
    24841 atttttttct tatcacctaa agctgaacgg agccctgtca caaccacttt ttacacacca
    24901 ctgctttgca gtttgtacat atctgcatac aaaccatttt gcgctagaag tgtttcatga
    24961 ttcccacgtt ctactatatg acctttgtct aaaactaaaa tcaattctgc atcccgaatc
    25021 gtggacagcc gatgggcaat cgcaatcgtt gtccgtcctt gtcgcatttt tgtcaatcct
    25081 tcttggatca atccttctgt ttcagtatcg atatttgctg tcgcttcatc caatactagt
    25141 attttaggat ctgtcacgat tgttcgcgca aaagtgatca gctgtcgttg tccgctagaa
    25201 tagctggcac cgtgttcgat cacttttgag tgatatcctt ttggtaattc atcgatgaac
    25261 ttgtttgctt gaacaaactc agctgcatgg cgaatctgtt cgtctgtgat agttggattc
    25321 atcatccgga tatttcctgc gatatctcca taaaacaaga aggcatcttg caagaccaat
    25381 cccattttct tacgtaactc ttccattgga tattctcgaa tgtcatgatc atcaatcaag
    25441 atctggcctt cataaaattc gtaaaaccgc atcaatacgt tgatgatcga gctttttccg
    25501 cttccggtgt gccccactaa tgcaacggtc tctcccggat tagcgacaaa agagatattg
    25561 ttcaatacat tgtgctctcc atcataagaa aagcttacat tgcgaaactc gatcttacct
    25621 gaagtaatcg cttgatctgc tccaggattt tgttgcggtg catattcttc tgtatccatg
    25681 attttcaaaa tacgactacc ggcaaccatt ccatctgtaa aggtgctcaa aaagtccatc
    25741 atttgagaca ttggattaaa aaatccctgg acataggtcg taaatgcata gatcagtcca
    25801 acttctacgg gagaatgcag cgcatcgatc ccaaacaaag tcaacgccaa cgcaatcgcc
    25861 aatgcatata gaaaattgat gatcggactt aacagaatcg aatttgtttt gatcatcgca
    25921 tagcgcgtgt ctaaatactc gttgttcact tgttcaaatt ctttagctaa acgagcttct
    25981 tggcgaaact gctggataat acgcatgccg gaaatcgatt cattcaattt cgtgttcagc
    26041 tgacttaatt tttcccgcat attccggtaa atacgagaac tgaatttctg gtaataccag
    26101 atgacgattc ctaaaatcgg caagaacacc aaagtccaca aggcaatttc tccattgatt
    26161 tgaaacatgg caatgaagga agaaacgact gcaaagatcc cagtcaatac catcaaaaag
    26221 acataccaaa attcaaatag cgtttctgtg tcatttgtca ctcgagaaac agtcgatcct
    26281 gccggtgtct gatcaaaata acgcatacct agtgtatgca gcttttcaaa taatcttacc
    26341 cgaacatact gataggtctt caatgaagcc atggaataca aataccattg gaaaaaccaa
    26401 atgatacttt tgactatgac tccaaacaag tacataccag caaaaaacaa aataacttgt
    26461 gtcgtagccg actgtttagc cagatgatca tccataaaag tttggatgat ccttggcaat
    26521 aggacattga tcacagacag ggcaaacgca aataagatag caacgatgaa cgttcttcta
    26581 aaaggtttgg cgaacttcat cagtcgtttg atgatcttct tctgttcttt aagtggaatc
    26641 gattttgtcc attccgattg ataattttca tccatcaggc ctcgcctcct tctatcttcg
    26701 cttctaattt ctgtttctcc cacattcgtt cataccagcc gcctaatgca agcagctcat
    26761 gatgggttcc tcgttcaatg attttccctt gatcgacaac aatgatttct tttgcatgca
    26821 tcacgctgct taaacggtga gctgcaatga tggttgtttt ctcttttctt atttctttta
    26881 aattatgcaa gatagcttct tctgttttgg catctactgc agaaagtgca tcatctaaga
    26941 tcagcaattc aggatttttg atcaatgctc gagcaatcga aatcctttgt ttctgtccgc
    27001 cagataaaga gactccacgt tctccaacta aggtatcata accttttggt aattccttga
    27061 tttcattgtt gataaatgct aaagaagccg catgctctac ttcttcttct gaaagttttg
    27121 gattagaaaa acggatatta tctcgaatgg tcatcgaaaa caagaagtga tcttgaggca
    27181 catagccgat tgatcctaat aaagcattca atgaatattc tttgatcgaa catccgccaa
    27241 attgaatttt tccttgataa tcatcgtatt cacgcatcag taatttgatg attgtcgttt
    27301 ttcccgcacc agttttccca acaataccta atgtttctcc ttcttggatc gcaaatttga
    27361 tttgttctaa tgctggtgat tcttctttcg gatagttaaa agaggaaata gctacattca
    27421 gctcgccttt tgccggagtc tgcactgcac ccagttgttc cacgatatgc gtcttttcat
    27481 gcaaaagttc attgacgcga tcataagaag catttcctct ttccaatacg ttgaacaatc
    27541 gaccaatcgc aaacatcggc caaacaagca taccgatata gctaataaat gagaccatct
    27601 gcccgattga gatcgtgcct tccaagacat actttccacc taaaataatt gttgcaacat
    27661 aagacagacc gataatcaag gtaatgaatg gatcaaataa ggagtctaaa aaattgactc
    27721 gtttgttttt aataattgca tcttcaatct tttcttcaaa atccgcgaga tcttcttttt
    27781 cttgtccaaa tgttttaatg actttgatcc cagtaatact ttcttgtgtc ttatcattga
    27841 tactagaaaa agcttcttgc gaatcacgaa aagcatcgtg gagttttgac cccagcactc
    27901 gtgaagtaac ggctagtaaa ggcagaggaa tcagcgcaat cagtgttaag cgccaatcaa
    27961 taaacaaaat catcgcaatg atcgttgttc cgcccgtgat cactgaatct gcaaaggtca
    28021 agataccagc tcctgctaca ttttggattg cattcaaatc attcgttgca tgagccatca
    28081 aatctccagt ccgatatttt tggtaaaaga gattgtccat ttctgtaaaa tgtttgaata
    28141 gctgctgccg aagttctttc tccaaccgag ctgcacttcc ccaaatattc gtccgccaga
    28201 tataacgaaa aagatattgt aagagagctg caaggatcaa taataagatc caccaggaaa
    28261 tgatctttat tgataaattt ttatcggcga tctcatcaat gatgatccca atcacttttg
    28321 gaggagctaa ctgtaccaag gccactgcga atagggagaa aatcccaatt aaataatgtt
    28381 tcttttcctg tcgaaaaaac caacctaatt ttctaaaaat cgacatttta ctcctccttt
    28441 gtctttatca agagcatagg attgtctgtg cttgtctttt ttgctgaata gtctgaatag
    28501 tctaacaaat tacgaaataa aaaacggact gaaaaaatca gcccgcaaaa agtctttggg
    28561 tcagaaccta ttttttccct tggttcttca tttgctgcat catctggcga attttcttct
    28621 cagatggttt ttgccccatt gacatcatca ttgatcgcaa catgtcctca ttaacgggag
    28681 gatttttttc taaatagtct ttcatatatt tacgcgctag gaaaaagcca cctactgcgc
    28741 cgattagcaa agcaataata gcaataagta cgacaatacc tgttgatacc atataaatct
    28801 ctcctttcta tttccgaatt gctctcaatg atttatttta ctaaagttta ttgaaaaaag
    28861 aaaggcgtat tctttagttt catgcaaaaa atagtggttt ttttagagaa gaacaaaatt
    28921 tctttcatta accagcaaga cacgccacga aaaaaaatta ggcataccaa atctttttct
    28981 ttaatttttt ctttctgagg tgtagaatgt aatcgtagat aaataggaaa ggtggttttt
    29041 tcttgaataa tcaatccaac aaacaatttc ataagcatcg acttattgaa catacaaatg
    29101 gtttatcttt agctgaagta aatggaacag tggatgtacc caagggaaaa agttttttta
    29161 aaacacttct tgcttactct ggaccaggtg cattagtagc agttggttat atggatccag
    29221 gtaactggtc cacttcgatc actggtggac aaaatttcca atatttattg atgtcagtta
    29281 ttttgatgtc tagtctgatt gctatgcttt tacaatatat ggcagcaaaa ttaggaatcg
    29341 tgacccaaat ggatttggcc caagcaatcc gagcacggac aagtaagaca ttgggtgtca
    29401 ttttatggat cttgactgaa ttagccatta tggcaacaga catcgcagaa gtcattggtg
    29461 cagcaatcgc cctctacctg ctttttgata ttccattggt aattgccgta tttatcactg
    29521 tgttagatgt tttcgtcctt ttattgctga caaaagtcgg gtttcgtaaa atcgaagctt
    29581 tggttgtctg cttgattttc gtcattttat tcgttttcgt ctatcaagtt ggcttgtcta
    29641 accctgattg gcacagtttg tttgccggat ttatccctaa cagtcacaca ttctcagact
    29701 caccggtcat cggcggcgaa actccgctga ctggagcttt agggattatt ggtgcaacag
    29761 tcatgccgca taacttatat cttcattcag cagtttctca aactcggaag atcgatcacc
    29821 aagacgaaga agatgtagca caagcggtac gcttttctac ttgggattct aatatccagt
    29881 tgtcaatggc ttttctagtc aatgcactgc tattgatcat gggtgtggct gtattcaaaa
    29941 ccggttctgt ccaagatcct tccttttttg gtctgtatga agctttatct gatccatcag
    30001 ctatgagcaa tggtatcttg attgctgtag caaaatctgg tatcttgtct actttatttg
    30061 ctgtagcact tcttgcttct ggccagaact ctactattac tggtacgttg accggtcaag
    30121 tcatcatgga aggcttcatc catatgcgta tcccaatttg gctaagaaga ctagtcacac
    30181 gattgctttc tgtgattcct gtattgattt gtgttttgtt tacaagtaca aaaggaacga
    30241 tcgaagaaca taccgctttg aataacttga tgaatgaatc ccaagttttt ctggcttttg
    30301 cattgccatt ctcgatgatt cctttactga tgatgaccaa tagccaagcg gagatgggga
    30361 gccgattcaa aaattcattt gttgtaaaac tacttggctg gttctctgtg attagtctga
    30421 cttacttgaa tttacgagga ttaccaggtc aaatcgaaag tttcttcggc gagcaagcca
    30481 cctcagctca actagcattg gctgatcaaa tcgcttatgt aatcattgtt cttgtccttc
    30541 tcctgttggc atggacgatt ggtgatttat ataaaggaaa caaaaaatat gaacagcgtt
    30601 tgcaggaaac gatagaagaa atcgagtaga aactgattct tttacacaaa ggaggttgtg
    30661 tcagaagtgg gcagcttcaa gaaataagaa ggaatttaag aaaattgctt ttcaaatttt
    30721 tgtgaaattt cggcttattt ccgaagaagt tacttctgga acaccgtgta tcagaggttg
    30781 tgaaaaagct gccttgcatc aagtaataag aacagccaag taaaaacagc tttttatgtt
    30841 tttaacttgg ttggacttat taccgcagat gcaagctttt gagcaccgtt tattcggaaa
    30901 aaggaactgc gacaagactt ttgtcacagt tccctttcct acgattagtc aagtaaaagg
    30961 tgtaagaaaa atagctcata aacgaaagcg acaaaatcaa
//
LOCUS       DASHHB010000031        29099 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.031, whole genome shotgun sequence.
ACCESSION   DASHHB010000031 DASHHB010000000
VERSION     DASHHB010000031.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 29099)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 29099)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..29099
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.031"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1030)
                     /locus_tag="U2K71_001579"
     CDS             complement(<1..1030)
                     /locus_tag="U2K71_001579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7315664.1"
                     /translation="MKSNEKTPLTTSLILLLSITCGVVVANMYYIQPIGTKVAASLSV
                     STSAVGILTMLTQLGYALGLLFLVPLGDVVDRPKLIIRMVALSAVSLLAAFFAPSFTL
                     FACASFLIGLLSIVPQIIIPYGAVLAGPKARGKVMGTLLSGLLVGILLSRTVSGIVAS
                     LFSWRMIYLFALCAVGILTLLLYWKMPRSVQQSSTAVSYLDSLKSLPHLVASQRLLRE
                     AAVSGFFMFGTFSIFWSTLIFYISSPAYNWGTFEAGILAIFGLSGAVAAPIVGRLADS
                     YSERKIVYMGLVMQTISFIALLVGGSHLAILLLAIILLDVGNQFGQVANQTRVQGLGE
                     AASNRNNTV"
     gene            complement(1112..1801)
                     /locus_tag="U2K71_001580"
     CDS             complement(1112..1801)
                     /locus_tag="U2K71_001580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEM7315665.1"
                     /translation="MKKKIIRLMLISLGIILILVVGYRLYLEYQTDIQLFLNPRASKA
                     ELLAHIRSHGTFASILLIVLTAVMCAVPGIPTSVVGVIAGLSFGPLTGAIINILGNAL
                     GNITAIFLMHRLKFLDKKTETNHWVQAIRQMKHPKIGVMLGYMIPVIPSSMVNFAADA
                     MKLPLQQIILSIVIGVIPSSVLYACGGEALFHGYNKTAVTLVASVTVLVGLVVFIYKD
                     RKKKQGKQKHK"
     gene            complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="U2K71_001581"
     CDS             complement(1907..2275)
                     /gene="rplL"
                     /locus_tag="U2K71_001581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L7/L12"
                     /protein_id="HEM7315666.1"
                     /translation="MALNIENIVAELKEATILELNDLVKAIEEEFGVSAAAPVAVAAA
                     GGAAAAEEQTEFTVELTAAGDQKVKVIKAVREATGLGLKEAKAVVDGAPAPVKEGVSK
                     EEAEELKAKLEEVGASVTVK"
     gene            complement(2328..2831)
                     /locus_tag="U2K71_001582"
     CDS             complement(2328..2831)
                     /locus_tag="U2K71_001582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L10"
                     /protein_id="HEM7315667.1"
                     /translation="MSEAIIAKKAAIVDEVSEKFTAAASVVVVDYRGLTVDEVTRLRK
                     QLRDANVEMKVIKNSILSRAAKKAGLEGMDEVFTGPTAVAFSNEDVVAPAKIMDEFAK
                     EAKALEIKGGIIEGKVSSLEEITALAKLPNREGLLSMLLSVLQAPVRNVAYAVKAVAD
                     KEDGDAA"
     misc_feature    complement(3035..3162)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00557"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L10 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00557"
     gene            complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="U2K71_001583"
     CDS             complement(3241..3930)
                     /gene="rplA"
                     /locus_tag="U2K71_001583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HEM7315668.1"
                     /translation="MAKKSKKMQDALKKVDSSKVYPVAEAVALAKETNIAKFDATVEV
                     AYRLNVDPKKADQQIRGAVVLPNGTGKTQTVLVFAKGDKAKEAEAAGADFVGDDDMVQ
                     KIQNGWFDFDVVVATPDMMATVGRLGRVLGPKGLMPNPKTGTVTMDVTKAVNEVKAGK
                     VTYRVDKAGNIHVPIGKVSFDDEKLVENFAAIHDVLLKAKPSAAKGQYMKNVTITTTF
                     GPGIHVDQASF"
     gene            complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="U2K71_001584"
     CDS             complement(4034..4456)
                     /gene="rplK"
                     /locus_tag="U2K71_001584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HEM7315669.1"
                     /translation="MAKKVEKIVKLQIPAGKATPAPPVGPALGQAGINIMGFTKEFNA
                     RTADQAGLIIPVVISVYEDRSFTFVTKTPPAAVLLKKAAKIEKGSGEPNKNKVAKVSS
                     DQVREIAELKMEDLNAANVEAAMRMVEGTARSMGITVE"
     gene            4799..5467
                     /gene="sdaAB"
                     /locus_tag="U2K71_001585"
     CDS             4799..5467
                     /gene="sdaAB"
                     /locus_tag="U2K71_001585"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent
                     subunit beta"
                     /protein_id="HEM7315670.1"
                     /translation="MKDLRYRSVFDIIGPVMIGPSSSHTAGAARIGKIIRSIFGEQPD
                     SVDIYLYESFAKTYRGHGTDIALVGGLLGMEPDDERLADSLKLAYEAGMEVLFVPKSE
                     KAEHPNSVKMLLKKGTRKLSVTGISIGGGNIQISELNGFKISLSMGTPTLIVVHQDVP
                     GMIAKVTSILSETNTNIGTMTVTRESKGENAIMIIEVDDPQVEETVKRLKQLPNIDSV
                     NYFD"
     gene            5480..6352
                     /gene="sdaAA"
                     /locus_tag="U2K71_001586"
     CDS             5480..6352
                     /gene="sdaAA"
                     /locus_tag="U2K71_001586"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HEM7315671.1"
                     /translation="MFLSIEELVDQAKAYPSVAELMIAVEMEMSGRSRSHIIEIMEKN
                     LSVMEQSINEGKEGVTSVTGITGGDAKKLDDYLNEADFLSGETILKAVRNAVAVNEVN
                     AKMGLICATPTAGSAGVVPGVLMAVRDRLDLTHEQQLEFLFTAGAFGLVIANNASISG
                     AEGGCQAEVGSASAMAAAALVCAKNGTPHQAAQAVAITLKNMMGLICDPVAGLVEVPC
                     VKRNALGSSQAFISADMALAGIESVIPPDEVVAAMYQVGRQMPSIFKETAEGGLAVTP
                     TAKRLAEEILNKRA"
     gene            complement(6443..7462)
                     /locus_tag="U2K71_001587"
     CDS             complement(6443..7462)
                     /locus_tag="U2K71_001587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy-acid oxidizing protein"
                     /protein_id="HEM7315672.1"
                     /translation="MEAGAFGYIRGGSEDEWTMKENTTSFNTKKIMPRILRGIDSADL
                     HTSVFGIELDTPIIQAPSAAQGLAHEKGEADTAKGVAAAGSIFSISTYANTTIKDAAD
                     AAPGAPQFFQLYMSKDDRFNEFILNKAVEAGAKAIILTADSTLGGYREEDVINQFQFP
                     LPMPNLAAYSEQSASGNGEGKGIAEIYAAAKQGLTPDDIKTIKEITHLPVIVKGIQSP
                     EDAVIAISAGADGIWVSNHGGRQLDGGPASFEVLPKIAEVVNKRVPVIFDSGVRRGEH
                     VFKALASGADLVAIGRPVIYGLNLGGAEGVTSVFEHLNKELSITMQLAGTKTIDEVKN
                     TKLMD"
     gene            complement(7635..8540)
                     /locus_tag="U2K71_001588"
     CDS             complement(7635..8540)
                     /locus_tag="U2K71_001588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEM7315673.1"
                     /translation="MERKRCMTEPIKNLCKRATAAALVLISLFALSGCQTTKINTTEV
                     SDKEKTAESSKVIEKTTIPTLFFHGYSGTENSFKGMLRRLEKNQQAVKELVLTVGTDG
                     QIQATGDLSGKADNPMVQVLFEDNKNNEWNQAEWIKTCLLYLKENYGIDKVNIVGHSM
                     GGVSALRYLGTYGQDTSLPQVQTLTAIGAPFNDFVDDSAQSLTDELAKGPAVVSNRYQ
                     DYQQMIGNIPITTRFFLIAGQLDETDLSDGTVPLNSALAVYALLKQRGNEIEEKVVTG
                     ENASHSMLHENQEVDQLVSRFIWKE"
     gene            complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="U2K71_001589"
     CDS             complement(8599..9144)
                     /gene="nusG"
                     /locus_tag="U2K71_001589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HEM7315674.1"
                     /translation="MESFEKNWYVLHTYSGYENKVKANIESRAQSMKMADFIFRVVVP
                     EEKETEVKNGKEKEIVHKTFPGYVLVEMIMTDASWYVVRNTPGVTGFVGSHGAGSKPA
                     PLLPEEVNHILRSIGMSLRQNDLEVEVGEVVKIIEGAFAGLEGQVTEVDGEKEKLKVN
                     IDMFGRETSTELDFDQVDKID"
     gene            complement(9245..9415)
                     /gene="secE"
                     /locus_tag="U2K71_001590"
     CDS             complement(9245..9415)
                     /gene="secE"
                     /locus_tag="U2K71_001590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HEM7315675.1"
                     /translation="MKFLKSVKDEIKQVTWPSRKQLRKDTLVVIETSIIFGLMFYVMD
                     TAIQSLFGWILK"
     gene            complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="U2K71_001591"
     CDS             complement(9439..9591)
                     /gene="rpmG"
                     /locus_tag="U2K71_001591"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L33"
                     /protein_id="HEM7315676.1"
                     /translation="MAKKKAALACSVCGSRNYTKSVSEGKSGERLEINKFCKYCNQYT
                     LHKETK"
     gene            9753..10397
                     /locus_tag="U2K71_001592"
     CDS             9753..10397
                     /locus_tag="U2K71_001592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM7315677.1"
                     /translation="MLLKTLVYKKKDLTTVTETSTLEEALKILETSGYRCVPILDSSG
                     NIFRGNIYKMHIYRHKANGGDMTLPVTHLLKNATKFIYLDTSFFKVFFTIKELPYIAV
                     LDSENHFYGILTHSTLLNMLSQSWSVEQGSYVLTIASTGKQGDLAAIAKIISKHSSIA
                     SCITLDIEKDEYIRRTLITLPANTEAKVCETIVNHLEKKNYKVVEIENLQNQEA"
     gene            complement(10453..10956)
                     /locus_tag="U2K71_001593"
     CDS             complement(10453..10956)
                     /locus_tag="U2K71_001593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEM7315678.1"
                     /translation="MQKTKSRQRLSVIVVNGIIMALYLALTILVAPVSSGPIQFRISE
                     SLNHLVVFNRKLLWGVLGGVIVYNAFFGFGIMDVVFGGGQTLLSLGLTALLQNKVKNV
                     KMRLVLNTIFFTISMGLIAYMLVPTGGQAFWTTYGTLALSEFIVMAVSAPLMYYLDRA
                     LDFEKRV"
     regulatory      complement(10969..11015)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            11553..12518
                     /locus_tag="U2K71_001594"
     CDS             11553..12518
                     /locus_tag="U2K71_001594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family lipid kinase"
                     /protein_id="HEM7315679.1"
                     /translation="MKFHYHILINPSAGSGNGYKVAERILPVLKNKHIDYTLHYSEYK
                     GHDAEIAETLAKETLIPWIEEEQKTIDTFPLLIIVGGDGTLHQVLDTFYQMEVEFPVA
                     YIPAGSGNDFARGAGLPKNPKKGLQLILAAQSPEKVHIMAYEEKISEKKGIAVNNFGI
                     GLDAAIVHATNHSSTKKRLNKYNLGSFSYLFSILRALFTQKGFPILVDAGGKQLSFSN
                     AFLCTATKHPFFGGGIAIVPTADASKPVIDFVVVERINFFKILWLILLLIQKKQLKSK
                     YFHHYTTNRLRIVSTTPQHGQEDGEEMEKRPFDITITTTKQLFWC"
     gene            complement(12617..13789)
                     /locus_tag="U2K71_001595"
     CDS             complement(12617..13789)
                     /locus_tag="U2K71_001595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315680.1"
                     /translation="MTLYKTIWTLLKANKSNLLIGVIITVVATFFFAQNIDVENTSLQ
                     DAKIALISEEESPVVDGLRKYLNDKETVVTLDGTSQKEIDDALYFNRVNIILHLPADF
                     TEQVEVGKMATIKIQSRPDAFSKTVVTQQVNTFLQTLILFQEEDKTIDAAMEQTQMAL
                     SVTGEVELSAGYSQRMKKLLTGTTFNFLSYGLFLSIFSGFSVINLAFNRKEISKRNQS
                     APITKRNLNRKVTFSLISYSLLLFSGFLLLMVLLVIHTSWDITVWYHILNTFLFLLPI
                     ISFSACITSLVKNSETSGGIKDIFITGSCFIGGVFVPAEYLPEMVSKIAAFTPTYWFV
                     QNNNLISETLSYNQTFAESFWFNGCVLVAFAAVFSMIQFILGKERNYRWQPNWAKN"
     gene            complement(13776..14921)
                     /locus_tag="U2K71_001596"
     CDS             complement(13776..14921)
                     /locus_tag="U2K71_001596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315681.1"
                     /translation="MFWHFYKYRTKILLRNKSMLFWTLAFPILLGLMFMAAFGEIDQG
                     GNLETINTGIVIKADQTALSENFTTVLEQVEVNDKPLFEVQELSEVEANEKLNADDLA
                     GFYLIDGSSVSLHVGQAGMSQTLMKNVMDQFLQRIGIISSKVAEVENVDLAPIMTVFE
                     QEVSFQEVHAGRNMSFKSFYFFTLIGMAILYGTMWGVRNVQDQQANQSSNGIRLSLIP
                     RKQSLVSIANLAASFTIVLTEIYIMLAVFRFIYQVDFGERWQWLMIVTALGSLCAILL
                     GTLVGNLIPKMNLVQKDSILISVTIAMSFFAGMMGSEQVKYWIDLHIPIVGQLNLVNL
                     ISESLYKLYFYTDLASFYTNLLWLIGFIFLFALGNAWMERGVRYDAL"
     gene            complement(14935..15867)
                     /locus_tag="U2K71_001597"
     CDS             complement(14935..15867)
                     /locus_tag="U2K71_001597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315682.1"
                     /translation="MIIEIDKLVKRYGDFVALNHFDLQVQEGEVLGLLGPNGSGKSTA
                     INCMLSLLNYDKGTIKLFGTTMTPENYAVKAMIGVVPQDVAVFDELSVQENIDYFCGL
                     YVSEKEKRKALIEEVIQLVGLEKYVSFRPGELSGGLKRRLNIACGIAHKPRLIFLDEP
                     TVAVDPQSRNKILESIKELNRQGATIVYTTHYMEEVEILCDQIVIMDQGQVIAKGTKE
                     SLKDMVRTADRLTIDIPLLPENVLSAIQRTIASDGIAYKKGQLQVTTTELNTVLLPIL
                     TILQEEKISYGKIATKEASLNDVFLEITGKDLRD"
     gene            15984..17063
                     /locus_tag="U2K71_001598"
     CDS             15984..17063
                     /locus_tag="U2K71_001598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GHKL domain-containing protein"
                     /protein_id="HEM7315683.1"
                     /translation="MHKSRLLNETSFLLVISLLLLVSQGFVAMQVIYLLIAVLFTGVS
                     YLLPTKPNWIFQVILFLLCAFLPDLVLFLPATMRVIAPEESHLDYLFLLGFLLISTVG
                     NHPPIKVAFVVILLFFSFYLRKKDQRTTDLEKKYIVSKDTHWEKQQELSEQNKALALS
                     QETQLALQIVEERNRIARDIHDNVGHLLSSAILQIGALEMINQEPKLKEPLENLSATV
                     HTGMDRIRQSVHDLHETSISFQHSLEFLIADFPSSVTIEGNLFEGLNETQQNCFLMVI
                     KEALTNSIKHSHAKTVVLHFRTLPAFYRLQITNDGTSPQKNSAPGIGLKTMRQRVQEQ
                     KGQVHIFQENQQFQLTIILPREGHQ"
     gene            17060..17678
                     /locus_tag="U2K71_001599"
                     /pseudo
     CDS             17060..17678
                     /locus_tag="U2K71_001599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325326.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(17788..18015)
                     /locus_tag="U2K71_001600"
     CDS             complement(17788..18015)
                     /locus_tag="U2K71_001600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM7315684.1"
                     /translation="MAKETEDKLAKLAKESGNDFKKLDEMEKEFKLNESPDVERDDRE
                     ESKLEKTLDKAPIFPPNTPNNPTNPGSPANP"
     gene            complement(18118..20706)
                     /locus_tag="U2K71_001601"
     CDS             complement(18118..20706)
                     /locus_tag="U2K71_001601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent RecD-like DNA helicase"
                     /protein_id="HEM7315685.1"
                     /translation="MQPEEMPYFVGTVAAIFFQNPSNFYKVLLVHISETTADYREKEI
                     VVTGSFGDIQENETYRFYGELVDHPKYGRQLKLERYEQEKPTTANGIVAYLSSEKFPG
                     IGKKTAEKIVELLGEDAIEKIIADPSVLKQISGLTKPKREMLAETIRLNHGMDQVIMG
                     LNRYGFGSQLAFSIYQAYKNESLDIIEENPYQLVEDIEGIGFKRADNLAEQIGIAADS
                     PRRIRAAILHQIFQHAVQTGDTYVPAETLLQQTIRILETSRPIEIQPDQVATVIIQLV
                     EEGKIQQEETNLYENSLYFSEWGIGNSIQRLLSRKKEIHYPEKEVQKNIRRIEKIFGI
                     QYGPSQEQAIAEAIRSPLFILTGGPGTGKTTVINGIVNLFAELNGLELDPSKYTQEIF
                     PILLAAPTGRAAKRMNETTGLPASTIHRLLGLNGREKTPSVSTKELEGGLLIVDEMSM
                     VDTWLANTLFKAIPTNMQVILVGDKDQLPSVGPGQVLHDLLEIEQIPKMELNQIYRQG
                     DGSSIIPLAHEIKEGRLPKDFTKNQKDRSYFRSDAYQIEPLISKIVEKAKNKGFRAQD
                     IQVLAPMYRGPAGIDALNKMMQEIFNPGKPGKKEVHWNDTVYRIGDKVLQLVNTPELN
                     VFNGDMGEITGIILAKDSEDKVDELVIQFDANEVTYKRNEWNKITLSYCCSIHKSQGS
                     EFKMVILPMVHQYQRMLQRNLLYTAVTRSKELLILLGEEQAYVTCVANESASRMTTLK
                     SRIMDNDKMNITLRTKLAAYEDSLVGNDPFEDGNYTKEPRVVKEKLPVEDDSGTNETK
                     NQVMETVEPDLFSSFVGEEKKEEVEPAVSKDFVLTAEVIDNQLVDPMIGMENSSPYDF
                     IPAKNA"
     gene            complement(20726..21385)
                     /locus_tag="U2K71_001602"
     CDS             complement(20726..21385)
                     /locus_tag="U2K71_001602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM7315686.1"
                     /translation="MELYFTRHGKTQWNQERRFQGSNGDSPLLPQSYEEIKAFGKKVK
                     YVPFKAIYSSTAKRARDTAEGINKELARPVEIFYTDKLRELGLGTLEGQSIDEMYQKY
                     PDNLPNLRNHLDLYDPSPFHGETIESAITRIETVVADAVARHKDGPLLFVGHGASLTA
                     AIQWMTGKELSELREMGGLFNSSLTILETEEPCRLLPYELKVWNQTDFLGTETAPEPL
                     L"
     gene            complement(21434..21937)
                     /gene="trmL"
                     /locus_tag="U2K71_001603"
     CDS             complement(21434..21937)
                     /gene="trmL"
                     /locus_tag="U2K71_001603"
                     /EC_number="2.1.1.207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (uridine(34)/cytosine(34)/5-
                     carboxymethylaminomethyluridine(34)-2'-O)-
                     methyltransferase TrmL"
                     /protein_id="HEM7315687.1"
                     /translation="MNHIVLFEPLIPANTGNIARTCAATNTPLHLIEPLGFSTDDKHL
                     KRAGLDYWNDVNITYHSNLEAFLTFLGNRKLHLISKFAHKVYSDENFADGEEHFFLFG
                     KETTGLPETFMRENEEKCLRIPMNDEHVRSLNLSNTAAMIVYEALRQQGFPNLELTHH
                     YENDKLD"
     gene            complement(22158..23024)
                     /locus_tag="U2K71_001604"
     CDS             complement(22158..23024)
                     /locus_tag="U2K71_001604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM7315688.1"
                     /translation="MLKKIDRGRQFLAENHQLFRCPVCQEEVKKSDNGLICKNNHRFD
                     LSKKGTLYFLSHAVKTEYDQGMFVPRRRMIQSGMYQPVIETIAQHLPEEVSLLDVGCG
                     EGSFLNELSKLKKIQNAVGFDISKEGVYAATEQSAEAFWCVADLTNLPFADQTFNAVL
                     NIFSPSHYQEFQRVLSEDGEVLKVVPQENYLKELRQAFYPNQPEKQSYSNERVVAKFA
                     EAMKLTERQRVTYEFEIPEENQKDLLAMSPLEWQVEERIKADLYKNPLKKITIDIELL
                     KGTKRKRFSSSF"
     gene            23133..23774
                     /locus_tag="U2K71_001605"
     CDS             23133..23774
                     /locus_tag="U2K71_001605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HEM7315689.1"
                     /translation="MIKEFCAENYTSIPLAIARGADRIELCDNLAVGGTTPSTGVIEE
                     TLSYAGEKDIPVMTIIRPRGGDFVYNDIELKIMHTDLIEAKKLGTDGVVFGCLTPSGW
                     LDEEALELLIETAEGLQITFHMAFDSIPEDRQFEAIDWLVEHGVDRILTHGGPADKTI
                     VENFPRLKELIAYTDGRLIILPGGGVTAENAAHVAKELNVSEVHGTKIVDLQL"
     gene            complement(23836..25197)
                     /gene="mgtE"
                     /locus_tag="U2K71_001606"
     CDS             complement(23836..25197)
                     /gene="mgtE"
                     /locus_tag="U2K71_001606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HEM7315690.1"
                     /translation="MDENQELEERFAQLTQDLRENNIQAFRNSFLEMHIYEQGQFYQS
                     LTEENRQLIYSYLSPKELADMFDVIEEDNEHMKEYLDEMRPSYAADMLSEMYTDNAVD
                     LLNTLDKKQIAKYLSLMSADDASEIKELLHYEDETAGAIMTTEFVSIVANQTVRSAMY
                     VLKNEADVAETIYYIYVVNQEGQLVGVISLRDLIVNDDDAMISDLMSERVLSVHVGDD
                     QEDVAQTFRDYDFLALPVTDYDDHLLGIVTVDDIIDVIDDEAASDYSGLAGVNVEEIN
                     ENPVKAASRRLPWLITLLFLGMSTASLISHYEDLVSEASILAVFISLITGTAGNAGTQ
                     SLAVAVRRLAISDEKDHGFVRLVLAEVLTGVVTGAVTGITIFLIVGFWKHNFILGFVI
                     GMAMLFAITVANLAGSLIPMLMDRLGFDPAVASGPFITTLSDLTSVLIYFNIASLFMG
                     YFM"
     gene            complement(25215..26114)
                     /locus_tag="U2K71_001607"
     CDS             complement(25215..26114)
                     /locus_tag="U2K71_001607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM7315691.1"
                     /translation="MQFTWDYEKDHSQQVKYFLKEKGISKGLLAKIKFQGGQIKVNDQ
                     VENVLFSLAKDDKVTIVIPAEGEHETVLLDETPIDIVYEDEHVLVVNKPAGVSSIPAQ
                     YHPNGTMANRVKAYYKKQGYENQVIHVVTRLDRDTSGLMLFAKHGFAHAKLDMQLREK
                     KFVKKYQALVSGRLSDLSAHGHIDLPIARDYSSLLKRMVSDEGRSAQTEYWIEKRLTE
                     IALVDIQLHTGRTHQIRVHFSAIGCALLGDDMYGGRMDLGIKRQALHCYDLRFYHPFT
                     EELLEFKQPLAADMEAVVRRFEE"
     gene            complement(26116..26913)
                     /locus_tag="U2K71_001608"
     CDS             complement(26116..26913)
                     /locus_tag="U2K71_001608"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HEM7315692.1"
                     /translation="MKVAIVHNKEAKTIEVTERLNALLEQAGIQRDDHEPELVISVGG
                     DGTLLSAFHHYSHCLNDVRFLGVHTGHLGFYTDWRDYELEELVDSLRTNREQSVSYPL
                     LDVRISYLDETPDQHFLALNESTIKRANRTMVADVYIKNELFESFRGDGLTISTPTGS
                     TAYNKSVGGAVIHPSINAFQLAEIASLNNRVFRTLGSPIVIAHDEWLEIKLENTEDYL
                     VTVDQLNVAKANIRSIYYRIAEERIHFASYRHMHFWHRVKDAFISED"
     gene            complement(26891..27595)
                     /locus_tag="U2K71_001609"
     CDS             complement(26891..27595)
                     /locus_tag="U2K71_001609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase family protein"
                     /protein_id="HEM7315693.1"
                     /translation="MERDWEAFLAPYEQTVSELKVKLRGIRKQFREQNRHVPIEFVTG
                     RVKPVDSIVTKSQLRHIPMGRLEEEMSDIAGLRIMCQFVEDIHQVVEIIRKRKDMKVL
                     QERDYITNKKASGYRSYHLVIEYPVQLISGEKKILAEIQIRTLAMNFWATIEHSLNYK
                     YQGVFPEEMSERLQRAAEAAYQLDEEMSSIREEIQEAQRLFSHGRGKSDDVYYRTLLK
                     KESEREEAGDESSDRS"
     gene            27726..28301
                     /locus_tag="U2K71_001610"
     CDS             27726..28301
                     /locus_tag="U2K71_001610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CYTH domain-containing protein"
                     /protein_id="HEM7315694.1"
                     /translation="MTQMLEIEYKSMLTKQEYEIFIAHYQLTDKDFRVQTNIYFDTVD
                     EQLKKQNCGLRIRFVGHQAEYTLKTPAEKGRLETTDTFITEEAEAFIFEKRLPRSGAV
                     FQKLSDLNIDPASLIQTGKLTTKRAEFPIEEGLLAIDESWGDNLHDFELELEVGDASV
                     GKIAFINFLKRFSLPYRPAKNKIQRMLEAKN"
     gene            28419..29096
                     /locus_tag="U2K71_001611"
     CDS             28419..29096
                     /locus_tag="U2K71_001611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HEM7315695.1"
                     /translation="MIEIYLFVNPLGEYCFEMEQQLLQFIEEEYGTPSKEKMQFRFLP
                     LVNLQTIGDVMQQKGISQNNLEERNHLFSMTYSAALDCKAAQFQGKKKGRRFLIKLQE
                     AVGCNDIPYSQELAESIFEEIGGDLNMFKEDRQSDFVKEVFFSDQKIAREMGITKHPS
                     AVVYNYACDRDFGVLVEVKGTSSLWRITELCKTTDNSYQVFNGEHLHTNDSQFHSRTS
                     HLHLLSN"
BASE COUNT         8563 a         6167 c         5231 g         9138 t
ORIGIN      
        1 atacagtatt atttcggttg cttgctgctt cacctagccc ttgtactcgt gtttgattgg
       61 cgacttgacc aaattgattc ccgacgtcta ataaaataat agctaaaagc aaaatggcca
      121 aatggcttcc gccaaccaat aaagcaataa agcttattgt ttgcataaca agtcccatat
      181 acacgatttt tctttcagaa taagaatctg ccagtcgtcc cacgataggt gcagcaactg
      241 ctccagacaa accaaaaatc gctaatatcc ctgcttcgaa tgttccccaa ttgtaggcgg
      301 gactgctgat ataaaaaatc aacgtagacc aaaagatcga aaatgttcca aacatgaaaa
      361 atccactgac tgccgcttct cttaatagac gttgagaagc aactaaatgc ggcaaacttt
      421 taagtgaatc aaggtatgaa acagctgtac tactttgctg gacagaacgc ggcattttcc
      481 agtaaagcag aagtgtaaga attccgactg cacataaagc aaataaatag atcatccgcc
      541 aagaaaaaag actggcaaca ataccagata ccgtccgaga aagcaaaatc cccacgagta
      601 aaccgctaag caatgtaccc atgaccttcc ctcttgcttt tggtcccgct agtaccgcac
      661 cgtaaggaat gatgatttgc ggcacgatcg aaagtaatcc tattaaaaaa gaagcgcatg
      721 caaataacgt aaacgaaggc gcaaaaaaag cggccaacag agagacggca gataacgcta
      781 ccattcgaat gatcaatttt ggacgatcca ccacgtcccc taaaggaact aaaaacagta
      841 accctaacgc atagcctaat tgggtcaaca tcgtcaatat cccgactgca cttgtgctaa
      901 cagataaact agctgctact tttgttccaa tcggctggat gtaatacata ttggcaacaa
      961 caacgccaca agtgattgat aatagtaaaa taagcgaagt tgttaaaggt gttttttcat
     1021 tactcttcat tgatttcccc catttcctat cttagtttac aacttatgga aaagattgga
     1081 aatagaaaaa tacaactatt cgccacactt attatttgtg cttttgtttt ccttgttttt
     1141 tcttgcgatc tttataaatg aagacaacta accctactag cacagtaaca cttgcaacca
     1201 acgtgactgc agttttgtta tatccgtgaa acagagcttc tccaccacac gcatacaaca
     1261 cagacgaagg gatgactcca atcacgatcg acaatatgat ttgctgtaga ggcaatttca
     1321 tcgcatctgc agcaaaattg accatagaag atggaataac cggtatcata tagcctaaca
     1381 ttacacctat ttttggatgt ttcatttgtc ggattgcttg cacccaatga ttcgtttcgg
     1441 ttttcttatc taaaaatttt aagcgatgca taaggaaaat tgcagtgatg ttaccaagag
     1501 cattacccaa aatgttgatg atcgcacctg tcagtggtcc aaagctcaaa ccagcaatga
     1561 ctccaactac tgaagtagga atacctggaa cagcacacat caccgctgtc aatacgatca
     1621 agagtatcga tgcaaaagtc ccatgcgaac gaatatgtgc gagtagctct gccttactcg
     1681 ctctcggatt taaaaataat tggatgtctg tctgatattc taaatacaac cgatagccca
     1741 ctaccagtat cagaatgatt cccaacgaga tcaacatcaa tcggattatt ttctttttca
     1801 tatttcctcc tgaaaaaaat ctatattcat taaataatag tttaacataa aaacaaaaaa
     1861 gcaccgaaat ccaacatagg atctcagtgc ttttaaatag taaaaattat ttaacagtta
     1921 cagatgcgcc aacttcttca agtttagctt tcaattcttc agcttcttct ttagatacgc
     1981 cttctttaac tggtgcagga gcgccatcaa ctacagcttt agcttctttc aagcctaaac
     2041 cagttgcttc acgaactgct ttgataactt taactttttg gtctccagct gcagttaatt
     2101 ctacagtgaa ttctgtttgt tcttcagcag cagcagcgcc accagcagca gctacagcta
     2161 caggagcagc agcagatacg ccaaattctt cttcaatagc tttaactagg tcgttaagtt
     2221 ctaggatagt tgcttctttt aactcagcaa cgatgttttc aatgttcaat gccattataa
     2281 tttcctccgt ttaattgttc ttttttattt aatgctttat gcggcaatta agctgcatct
     2341 ccatcttctt tgtctgccac tgctttgaca gcgtaagcca cgttgcggac tggcgcttgt
     2401 agtacagata gcaacataga tagaagtcct tcgcgatttg gcaattttgc caatgctgtg
     2461 atttcttcca atgaagagac tttaccttcg atgataccgc ctttgatttc taatgctttt
     2521 gcttctttag caaattcgtc catgattttc gctggtgcga ctacatcttc gttactgaat
     2581 gcaacagctg taggtccagt gaatacttcg tccatgcctt caaggccagc ttttttagct
     2641 gcacgtgaaa gaatagaatt tttgataact ttcatttcta cgttagcatc acgaagttgt
     2701 ttacgtaaac gtgttacttc atcaactgtt aatccgcggt agtcaacgac tactactgat
     2761 gcagctgctg taaatttttc gcttacttca tcaacgattg ctgctttttt tgcgataatt
     2821 gcttcactca tttttgagtt tcacctcctg aattttgatg gaagagtttt ttgcactgaa
     2881 aaggcaagtc acttttatag ttactttgct caaccagtat tattcatcac ctattcactt
     2941 cgttcatagt gattctactc gctttgctcg aaaggaaact cctttcgctt tgctcaacca
     3001 gtattgttca tcatctcttc acttcgttca tagtgattca caataaaaaa ctctatgcca
     3061 ccgcagacat agagggacta ttgaatatat tcaataaacg tcctcggtag gaaattaagg
     3121 cttgatgcca cctactgtct tcggtacaag ttaattatta acctcaacta gcttagcatg
     3181 ctaaactagt tgagtcaaga attatttttc agttggatca atcaacattc taatatttaa
     3241 ttagaaagaa gcttggtcaa catggatacc aggtccaaat gttgttgtga tcgttacatt
     3301 tttcatgtat tgacctttag ctgctgatgg tttagctttt agcaatacat catggattgc
     3361 tgcaaagttt tcaactaatt tttcatcgtc aaatgaaact tttccgattg gtacatggat
     3421 atttccagct ttgtccacac ggtaagttac tttaccagct ttcacttcgt ttactgcttt
     3481 tgttacgtcc attgtaactg taccagtttt agggtttggc attaagcctt ttggtcctaa
     3541 tactcgtcct agacgaccta cagtagccat catatcaggt gttgcaacaa ctacatcaaa
     3601 gtcaaaccat ccgttttgga ttttttgaac catgtcgtcg tcacctacga aatctgcacc
     3661 agctgcttca gcttcttttg ctttgtctcc tttagcaaaa actaaaacag tttgtgtttt
     3721 accagttccg tttggtaata caacagcacc acggatttgt tggtctgctt ttttagggtc
     3781 tacgttcaaa cggtatgcaa cttcaactgt tgcatcaaat tttgcaatgt ttgtttcttt
     3841 tgctaaagct actgcttcgg caacaggata aactttactt gaatcaactt tttttaatgc
     3901 atcttgcatt tttttgctct ttttagccat tttggttcct ccttgattgt ggttataacg
     3961 gttgtacctc ccacgtgttc tatatctttc aatatagagc caactgagaa gctgctgtct
     4021 tagggtgtat agattattct acagtgatcc ccatgcttcg tgcagttcct tcaaccatgc
     4081 gcattgctgc ttcaacgtta gctgcgttta ggtcttccat ttttaattca gcgatttctc
     4141 ttacttgatc gctagaaact ttcgcaactt tatttttgtt tggttcgcct gaaccttttt
     4201 cgattttagc tgcttttttc aatagaacag cagctggcgg tgtttttgta acgaatgtga
     4261 atgaacggtc ttcgtataca gaaataacaa ccggaatgat caaacctgct tgatcagctg
     4321 tacgagcgtt gaattctttt gtgaatccca tgatattgat acccgcttga cctagtgcag
     4381 gacctactgg tggagctgga gttgctttac ctgcaggaat ttgcaattta acgatttttt
     4441 ctactttctt tgccacgaga catacctcct taagtccgtg atgtggttaa ttggagatgc
     4501 atatttctcc tcccacgttt tatctgatct gaaaaaatcc agacaaataa tgtgcaaaac
     4561 aatgcacact ggtacagtat agcaaattta tttgtttttt caagattttt ttctgctatt
     4621 tttacatttt gcttgaataa acgctaaatt tattctaaaa aaacaacaat ttttcgttct
     4681 catttcttct tttatgttac acttgcgcta gcatggttca acagatgaaa gcaaacaata
     4741 acacggtttt ttagaccctt gtacaaagat tcctacataa caaaatgagg tggagaaaat
     4801 gaaagattta aggtatagaa gtgtctttga tattattgga cctgtgatga ttggtccaag
     4861 tagctctcac actgccggag ctgctagaat cgggaaaatc atacgtagta tatttggcga
     4921 acagcctgat tctgtagata tttatctata tgaatcattt gcaaaaacct atcgggggca
     4981 cggaacagat attgcattag ttggcggcct gttagggatg gaacccgatg atgaacgttt
     5041 agctgattct ttgaaattgg catatgaagc cggtatggaa gtcttgtttg ttcctaagag
     5101 tgaaaaagcc gaacatccaa actctgtaaa gatgcttcta aaaaaaggaa cacgaaagct
     5161 ttcagtcact ggcatttcga tcggcggtgg caatatccag atttctgaat tgaatggatt
     5221 caaaatttct ttaagcatgg gtactcctac cttgatcgtt gtccaccaag atgttccagg
     5281 tatgattgct aaagtgacta gtatactttc tgagaccaat acgaatatcg gcactatgac
     5341 agtgacacgg gaatcaaaag gtgaaaatgc cattatgata attgaagtag atgatcctca
     5401 agtagaagaa acggtaaaaa gactcaaaca gctgccaaat atcgacagcg taaattattt
     5461 cgattaagga gctaaaaaaa tgtttttatc gattgaagaa ttagtagatc aagcaaaagc
     5521 ttatccaagt gtagcagaat tgatgattgc agttgaaatg gaaatgagtg gccgttcccg
     5581 tagtcacatc atcgagatca tggaaaaaaa cctctcagta atggaacaat caatcaacga
     5641 gggaaaagaa ggtgttacct ccgtcacagg aattaccggt ggtgatgcaa aaaaattaga
     5701 tgattattta aatgaggcag actttttaag tggagaaaca atcttaaaag ctgtacgcaa
     5761 tgctgtagct gttaatgaag tcaatgcaaa aatggggttg atttgtgcta caccgactgc
     5821 cggaagcgcg ggtgttgttc ctggtgtgct gatggctgtc cgagatcggc ttgatttgac
     5881 acatgaacag caattggaat tcttatttac cgctggcgct ttcgggttag tcatcgcaaa
     5941 caacgcttct atcagtggtg cagaaggggg atgtcaagca gaagttggtt ctgcaagtgc
     6001 aatggcagca gcagctttgg tttgtgcaaa aaatgggact cctcatcaag cagcacaagc
     6061 agtagccatt acattgaaaa atatgatggg actgatttgc gatcccgttg caggcttagt
     6121 tgaagtcccg tgtgtcaaac gtaatgcttt aggctcttct caagctttta tctctgctga
     6181 tatggcatta gcgggtatcg aaagtgtcat tccgccggat gaagtcgttg ctgcaatgta
     6241 ccaagtcgga cgtcaaatgc cgtcgatttt caaagaaaca gcagaaggtg gcttagctgt
     6301 tacgccaact gctaaacgac ttgcggaaga gattttaaat aagagggcgt gataaagctc
     6361 cttaatttcg agtattaaga gattagttat cttcataaca aaaaacgaag agaggactaa
     6421 ttgcccttct cttcgttttt tattaatcca tcaatttcgt attttttact tcatcgatcg
     6481 tttttgttcc tgcaagttgc atcgtgatcg acaattcttt gttcaggtgt tcaaaaacag
     6541 atgttactcc ttctgcccca cctagattca acccataaat cactggtcgg ccaatcgcaa
     6601 ctaagtcagc accacttgcc agagctttga agacatgctc tcctcgacgg acaccagagt
     6661 cgaaaatcac tggtacacgt ttgttgacta cttcggcaat ttttggcaat acttcaaagg
     6721 acgcaggacc tccatctaat tgacgtccac catgattgga tacccagata ccatctgccc
     6781 ctgcagagat agcgattact gcatcttctg gcgattgtat tccttttacg ataactggaa
     6841 gatgggtgat ttctttgatt gttttgatgt catctggtgt caagccttgt tttgcagctg
     6901 cataaatttc tgcgattcct tttccttcgc cattaccact tgcactttgt tcgctatacg
     6961 cagctaagtt aggcattggt aatggaaatt ggaattgatt gatgacgtct tcttcacggt
     7021 aaccaccgag cgttgaatct gccgttaaaa tgatagcttt agcccctgct tctactgctt
     7081 tgttcaaaat gaactcattg aatctgtcgt ctttactcat atataattgg aaaaactgcg
     7141 gtgcgcctgg cgctgcatcg gctgcgtctt tgattgtcgt atttgcataa gtgctgatgg
     7201 aaaagattga tccagcagca gctactcctt tagctgtatc agcttcccct ttttcatgtg
     7261 ccaatccttg ggccgctgat ggcgcttgga taatcggtgt atctaattca attccgaaaa
     7321 cagatgtatg caaatcggct gagtcgattc cgcgtaatat ccgtggcatg attttctttg
     7381 tattaaaaga agtcgtattt tctttcatcg tccactcatc ttcagaacca ccgcgaatat
     7441 agccaaaagc tcctgcttcc atgcgctctt ttacacgacc ttctagttag gcaatgttta
     7501 caatatcgat tggatgttct tcattacttg tatgataagt catagtagat ccttctttca
     7561 tcttcttgat tacatataaa tcatactaaa cttacaaaaa gtaagcaaac atttactctc
     7621 agatactttt atcgctattc tttccaaata aatcgactta caagctgatc tacttcttgg
     7681 ttttcatgaa gcatactatg agaggcattt tcacctgtca ctactttttc ctctatttca
     7741 tttccgcgtt gcttcaacaa cgcatagacc gctaatgcac tgtttaacgg tactgttcca
     7801 tcactcaagt ctgtttcatc tagttgacca gcaatcaaga aaaatcgagt ggttatagga
     7861 atattaccga tcatttgctg gtaatcttga tagcggttag aaacgacagc aggccccttt
     7921 gctaactcat ctgtcagaga ctgcgcacta tcatcgacaa aatcattaaa aggcgcgcca
     7981 attgctgtta atgtctgaac ttgtggcaat gacgtatcct gtccatacgt tcctaaatag
     8041 cgtagtgcac taactcctcc catggaatgc cccactatgt tgactttatc tatgccgtag
     8101 ttttctttca gatacaaaag acaagttttg atccattctg cttgattcca ctcgttattt
     8161 ttattatctt caaacaatac ttggaccatc ggattgtctg cttttccaga caaatctcct
     8221 gttgcttgta tttgtccatc tgttcctact gttaaaacca attcttttac tgcttgctga
     8281 tttttttcca aacgccgcaa catcccttta aaggaatttt ctgtcccgct gtagccgtgg
     8341 aaaaaaagtg ttggaatagt ggttttttct atcactttac tggattctgc cgttttttct
     8401 ttatcagaaa cttcagtagt atttatcttc gtcgtctggc aaccacttaa cgcaaacaaa
     8461 ctgataagga ctaaagctgc tgcagttgca cgtttgcata aatttttgat tggttctgtc
     8521 atacacctct tcctttccat atagaaaaaa gaaagacttg aaaggccaat catatgatcg
     8581 acatttcaag tctctttatt aatcgatttt atctacttga tcgaaatcaa gttctgtact
     8641 ggtttcacgt ccaaacatat cgatgttgac tttcaatttt tctttttctc cgtcaacttc
     8701 tgttacttgt ccttctaatc cagcaaatgc gccttcgatg attttcacga cttcgccaac
     8761 ttctacttct agatcatttt gtcgcaggct cataccgata gaacgcaaga tatgattgac
     8821 ttcttctgga agtaatggtg ctggtttgct tccggctccg tgtgaaccaa cgaatcctgt
     8881 cacaccaggt gtgttgcgga caacatacca tgaagcgtct gtcatgatca tttcaacaag
     8941 tacgtatcct gggaatgttt tatgaacgat ttctttttct ttcccatttt tgacttcagt
     9001 ttctttttct tcaggaacaa cgacacggaa aataaagtcg gccattttca tactttgcgc
     9061 acgagattcg atatttgctt ttactttgtt ttcatatcct gaatatgtat gcaagacata
     9121 ccagtttttt tcaaaagatt ccattgaagg ctcctcattt cattgtaaaa taaaaaaacc
     9181 tcagtacacc cgaagttttt ctttcgcttc attatagcac taaaagtacg ttgtggctag
     9241 cgagttattt taaaatccag ccaaataagc tttggatggc tgtatccatt acgtaaaaca
     9301 tcaatccgaa aataatagaa gtttcgatga ctactaacgt atctttacgg agttgttttc
     9361 tgcttggcca agttacttgt ttgatttcat cttttacgct ttttagaaat ttcatgagac
     9421 aacctccctt gctactgttt atttcgtttc tttatgtaat gtatattgat tacaatattt
     9481 acaaaactta ttgatttcca aacgttcgcc gctttttcct tcactgactg attttgtgta
     9541 gttgcgagac ccacaaacgg aacaagcgag tgctgctttt ttctttgcca tttacttctg
     9601 cccccttgct gagaatcaga cttatacttc ataacagtat cacgtaactt tttacttgtc
     9661 aatttgtgaa ataaactatc tattgttttg gataaaatta tgatatgatt aatttgaaat
     9721 taagctaaaa tgagacaatt ggaggtatag cgatgctatt aaaaacactt gtatataaga
     9781 aaaaagatct gacaaccgtt acagagacta gtacattaga agaagcatta aaaattttag
     9841 aaacttccgg ataccgttgt gtgccgatct tagattcttc aggtaatatt ttccgtggaa
     9901 acatttacaa aatgcatatc tatcgtcata aagcaaatgg cggtgatatg acgcttcctg
     9961 tcactcatct tttgaaaaac gctacaaagt tcatttacct ggatacttct ttcttcaaag
    10021 tattctttac gatcaaagag cttccttata ttgctgtcct tgacagtgaa aatcattttt
    10081 atggtatttt gacacatagt acgttgctta atatgctctc tcaatcttgg agcgtagaac
    10141 aaggaagcta tgtactgaca attgcttcca caggaaagca aggagatttg gctgctattg
    10201 caaaaatcat ttcaaaacac agcagcattg ccagctgtat tactcttgat atcgaaaaag
    10261 acgaatacat tcgacgtaca ttgatcacgc taccggctaa cactgaagcg aaagtatgtg
    10321 aaacgatcgt caaccactta gagaaaaaga attataaagt ggtagaaatt gaaaatttgc
    10381 aaaaccaaga agcataatca aaaagcgaaa aaagaaaaca gctaatctgc tttctttttt
    10441 cgctttttat aattagacac gcttttcaaa atcaagcgcg cgatctaaat aatacatcaa
    10501 tggtgctgaa acagccatga cgataaactc acttaatgca agcgtgccat aggttgtcca
    10561 aaatgcttgg ccacctgttg gaactaacat ataagcaatc agtcccatgc tgatggtaaa
    10621 gaagatcgtg ttcaatacta aacgcatctt tacatttttc actttgtttt gcaataaagc
    10681 tgtcaggcct aaagaaagca atgtttggcc gccaccaaaa accacatcca tgataccaaa
    10741 accaaagaag gcattataga cgatgacccc gcctaaaact ccccaaagca atttacggtt
    10801 aaagacaact aaatgattca aactttctga aatacgaaac tggatcggcc ctgaggatac
    10861 aggcgcaact aaaatagtta acgctagata aagagccatg atgatgccat ttacaacgat
    10921 tacggataac cgttgtctgc ttttcgtttt ttgcattttc tctcctcttt tctcctagtt
    10981 tttttaacgc gggatggttg atgaaccgcg gttatgtgaa acaaacaata attataacag
    11041 acttttcatt tatttcaatc cgtcttatct gcttttaaaa aagcaaaagc ttaaacaatg
    11101 taaacgtttg ctaaaaatcg tgggatatgg tataaagtaa gtgtaagggg tcataagaat
    11161 tcccagtaaa gcagacgaag taggatctgc aagaacagaa ttgttgtgaa acttaaataa
    11221 ataaaaaagg atgtgggaat ttgagcaacc tcttactaga gaacggtcta gcaagcagct
    11281 gaagatctaa gtggacatgc cgaaccttgg aacttctcct tacgtaagta atggatcatg
    11341 cgcttgctag gcttttttgt gtttttcggt cttcggacct ttttcatttt cctagacttc
    11401 tgctataata accaagaatt aattttgtaa cagatttttt tgaaaaaaca agtttctttc
    11461 attcaaaaaa tagaaaaagc ttgctaatta ttggttaacc agcaaacagg agtattaaaa
    11521 tatcttgttt tattgaatag caggaggaaa tcatgaaatt ccattatcat atattgatca
    11581 atccatcggc aggcagcgga aatggctata aagtagcaga aagaatttta ccggtattaa
    11641 aaaataagca tattgactat acacttcatt atagtgaata caaaggacac gatgctgaga
    11701 ttgctgaaac tttggcaaaa gaaacattga ttccttggat agaagaagaa cagaaaacaa
    11761 tcgatacttt cccccttttg atcatcgtag gaggtgacgg caccttacat caggtacttg
    11821 atactttcta tcagatggaa gtagaattcc cagttgctta tattccagca ggttctggaa
    11881 atgattttgc tcgaggggca ggccttccaa aaaatcccaa aaaaggactt cagctgattt
    11941 tagcagctca atcaccagaa aaagtccaca taatggctta tgaagaaaaa ataagtgaaa
    12001 aaaaaggaat agctgttaat aatttcggta taggattaga tgcagctatc gtccatgcga
    12061 caaatcattc ttccacaaaa aagcgattga acaaatacaa cttaggatca ttttcgtatt
    12121 tattctctat tctccgtgct ttattcactc aaaaaggatt tccaattctg gtagatgcag
    12181 gaggaaaaca attaagtttt tcaaacgcat ttttatgtac agcaacgaag catccttttt
    12241 ttggcggagg aatcgcgatt gttccaactg ctgatgcgtc taaaccagtc attgattttg
    12301 tcgtggtcga acgaatcaat ttctttaaaa tcttgtggct cattctttta cttatacaga
    12361 aaaaacaatt gaaatcgaaa tatttccatc actacacaac aaacagatta cgtatcgttt
    12421 caacaacacc gcaacatggt caagaagatg gagaagaaat ggaaaaacga ccttttgata
    12481 tcacaataac cacaactaaa cagttgtttt ggtgttaata aaagaggttt tgtcagaagc
    12541 tacatctgga acaccgttta ttcggaaaaa aggactgtga cacgactttt gtcacagtcc
    12601 tttttttact atgcagttaa ttctttgccc aattgggttg ccatcgatag tttctttctt
    12661 tacccaaaat aaattggatc attgaaaaca ccgcagcaaa agcaactaag acacaaccat
    12721 tgaaccaaaa agattctgca aatgtttgat tgtaactgag tgtttcactg atcaagttat
    12781 tattttgaac aaaccaatat gttggtgtga atgccgctat tttgctaacc atttctggca
    12841 agtattctgc tggtacaaac accccaccga taaagcaact gcctgtgata aaaatatctt
    12901 tgatcccacc gctggtttct gagtttttta ccaatgaagt gatacaagcc gaaaagctaa
    12961 tgatcggcaa taaaaacaag aaagtattta aaatatgata ccataccgtt atatcccacg
    13021 aggtgtggat cactaacaga accattagaa gtaagaagcc actgaacaac agcagcgagt
    13081 aactaatcaa actaaaggtt actttgcgat tcaagtttcg tttggtaatg ggcgcactct
    13141 ggtttcgttt actgatctct ttgcgattaa acgctagatt gatgacgctg aaaccgctaa
    13201 aaatggatag aaacaaccca taagacaaga aattaaaggt tgtgcccgtc aacagctttt
    13261 tcatccgctg actatatccc gcacttagct caacttctcc agtaaccgag agtgccattt
    13321 gtgtctgttc catagctgca tcgattgttt tatcttcttc ttgaaacaga atcagtgttt
    13381 gcaaaaaggt gttcacttgc tgtgtaacaa ctgttttaga aaaggcatct gggcgactct
    13441 gtatcttgat cgtcgccatc tttcctactt cgacttgttc ggtaaagtct gcaggcagat
    13501 gtaagataat gtttacacga ttaaaataaa gagcatcatc gatttctttt tgtgaggttc
    13561 catccaatgt aacgactgtt tccttgtcat tcaaatattt acgaaggcca tcgactacag
    13621 gcgactcttc ttcactaatc aaggcgattt tcgcatcttg cagagaggta ttttcaacat
    13681 caatattttg cgcaaaaaag aatgtggcaa caacagtgat aattacacct atcagtaaat
    13741 tcgatttatt tgctttaagc aatgtccaaa tcgttttata aagcgtcata acgcacccct
    13801 ctttccatcc aagcatttcc taacgcaaaa aggaaaataa atccgatcaa ccatagaaga
    13861 ttggtataaa aactagccaa atcagtgtag aaataaagtt tatacaaact ttcgctgatc
    13921 aagttgacga gatttaattg accaacaatc ggaatatgaa gatcaatcca atacttcact
    13981 tgttccgacc ccatcatacc agcaaaaaag ctcatcgcaa tcgtcactga aatcaagatg
    14041 ctatcctttt gcaccagatt catttttggt atcaaattac caaccaatgt acctaacaaa
    14101 atcgcacaca acgaccccag agcggtaaca atcatcaacc attgccaacg ttcaccaaag
    14161 tctacttgat agataaaacg aaagacagct aacataatat aaatctctgt caaaacaatc
    14221 gtaaagcttg ctgccaaatt agcgattgaa actaacgatt gctttctagg aatcaaggac
    14281 aagcgaatac catttgacga ttgatttgct tgctgatcct gtacattgcg gaccccccac
    14341 atcgtcccgt agagaatcgc cattccaatc agagtgaaga aataaaaact tttgaaactc
    14401 atattacgac ctgcatgaac ttcttgaaaa gatacttctt gttcaaaaac agtcatgatg
    14461 ggcgcgagat caacgttctc cacttctgcc actttactcg aaattatacc aatacgctgt
    14521 aaaaattgat ccatgacgtt tttcatcaac gtctgactca ttcctgcttg acccacatgg
    14581 agcgaaacag atgaaccatc aatgagataa aaaccagcca aatcatctgc atttagcttt
    14641 tcattcgctt ctacttccga tagttcctgt acttcaaata atggtttatc attgacttcg
    14701 acttgttcta agactgtggt aaaattttcc gaaagcgctg tttgatccgc tttgattaca
    14761 attccagtat taatcgtttc aagattgcca ccttgatcaa tctcgccaaa ggccgccata
    14821 aacatcaatc ctaaaagaat tggaaaggcc aatgtccaaa acaacatgga tttatttcgc
    14881 agcagtattt ttgtgcgata tttataaaaa tgccaaaaca ttatttcccc tcctctagtc
    14941 tctcaaatcc ttgccagtaa tctctaaaaa gacatcattc aaggacgctt ctttggttgc
    15001 tatttttccg taactgatct tttcttcttg taagattgta agaatcggca acaatactgt
    15061 atttagttca gtagttgtta cttggagctg acctttttta taagcgattc catcagaagc
    15121 tattgttcgc tgaatcgctg acaacacatt ttctgggagt aatgggatgt cgatcgtcaa
    15181 acgatccgcc gtccgtacca tatcttttag agattctttg gttcctttag caatcacctg
    15241 tccttgatcc atgatcacga tctggtcgca aagaatttct acttcttcca tgtaatgtgt
    15301 tgtatagaca atggttgctc cttgtcgatt cagctccttg atactttcta aaattttatt
    15361 ccgactttgg gggtctacag ctactgtcgg ctcatctaaa aaaattaacc gcggcttgtg
    15421 ggcaatccca catgcgatgt tcaaccgacg tttcaagcca ccactaagtt ctcctggacg
    15481 aaacgaaaca tatttttcta atcccactaa ttgaatcacc tcttcaatca aggctttcct
    15541 tttctctttt tcggaaacat ataaaccgca aaaataatcg atattttctt gcacacttag
    15601 ttcgtcaaag accgcaacgt cttgaggaac gaccccaatc attgccttga cagcataatt
    15661 ttcaggagtc attgttgtcc cgaacaattt aatggttcct ttgtcataat ttaacaaaga
    15721 aagcatacag ttgatggcag tcgatttccc actgccgttt ggacctaaca gacctaagac
    15781 ttctccttct tgtacttgca gatcaaaatg attcaatgct acaaaatctc catagcgttt
    15841 gactaattta tcaatttcga taatcatcat gaaaaactcc ttccaaattt cttgttgatc
    15901 tgactataat aaagtgcaag aaaagaaact agtgacgatt ggctaaacca cacatgacat
    15961 ttgtcacaat gaaggagtct gctatgcata aatctcggct actcaacgaa actagctttc
    16021 tactggttat ctcattgctt ttgctggtaa gtcaaggatt cgttgctatg caagttatct
    16081 atttattgat cgccgtttta ttcactggtg tcagttattt actgcctaca aagccaaatt
    16141 ggattttcca agttattcta tttcttttat gtgccttttt acctgatctt gtcctgtttc
    16201 tgccagcgac gatgcgagtg atcgcccctg aagaaagcca ccttgattac ttgtttttgt
    16261 taggtttttt gttgatttcg acggttggta atcatccccc catcaaggtc gcctttgtcg
    16321 taattttgct gtttttttcc ttttacttac gaaaaaaaga tcaacgaacg accgatcttg
    16381 agaaaaagta cattgtctcc aaagatactc attgggaaaa acaacaagag ttatcggaac
    16441 aaaataaagc attggccctg tcacaagaaa cacaattagc cttgcaaatt gtggaagaac
    16501 ggaaccgaat tgcccgagac atccatgaca atgtcggtca tctgctgtca agtgcgattt
    16561 tacaaattgg tgcgcttgaa atgatcaatc aagaaccaaa actaaaagaa ccattagaaa
    16621 atttaagtgc caccgttcac actgggatgg atcggattcg tcaaagtgtc catgacctcc
    16681 atgagacatc gatctctttt caacactcct tagagtttct gatcgctgat tttccttctt
    16741 ctgttacgat tgaaggaaac ttgtttgaag gattgaatga aacgcagcaa aactgcttct
    16801 taatggtcat caaagaagcc ttaaccaaca gtatcaaaca tagtcatgcc aaaaccgttg
    16861 ttttacattt tcgaacgttg ccggcctttt atcgcttgca aatcacgaat gatggcacca
    16921 gcccacaaaa aaattctgct cccggtattg gtttgaaaac gatgcgtcaa cgtgttcaag
    16981 aacaaaaggg acaagtccat attttccaag aaaaccagca atttcagtta acgatcattt
    17041 taccaaggga gggacaccaa tgataacagt agtcattatt gatgatgatc catttgtgac
    17101 gaaatcgtta caaaccattt tagaaagcac gaaagagatc cgtgtgttag gcattggtca
    17161 ttgtgccaag gatgcgcgaa acccatcgtc ccgatgtttt gctgacggac atccgcatgc
    17221 ctaaacaaac gggcatcgat gctgccaaag aaattttggc taaatttccc aaagccatcc
    17281 ttttgctttt gacgacattc aaagatgaag aatacatccg cgaagcattt tccatcggtg
    17341 tcaaagggta tttaatcaaa cagaacttgc aagcgattat tccttctgtc aaatccgctt
    17401 ataatggaca ggccgtattt ggtaatgaaa tcatcgagac tttcactcaa atgatgaaga
    17461 ataaaccgac gattgatagt catgcttatg cttcttttag tgaacgtgag cttgcaatca
    17521 tcaaagaagt agctgccggg aaaaacaata aagaaattgc agatgccttg tacttgagtg
    17581 acggcactgt tcgcaattac atcagccaat tactagaaaa gctagattta cgggatcgca
    17641 cgcaattggc tatctatttt tatcagcatt tgcaataagt cttatctaac acaaaaaaac
    17701 ctgaatgatg ctccttcgca tcattcaggt ttttttcaca tcttctattc aattgtcccg
    17761 caaaccattt cctcacgatt gaaagaatca aggattcgca gggctgccag gattcgttgg
    17821 attgttcggc gtgttaggcg gaaaaatagg cgccttgtct aacgtttttt ctagttttga
    17881 ttcttcacga tcgtctcgtt ctacatcagg actttcattc aatttgaatt ctttttccat
    17941 ctcatctaac tttttaaagt cattgccaga ttcttttgct aatttcgcta atttatcttc
    18001 ggtttcttta gccataaagt aacgcccccc ttttgatttc agcttagcat atcacactga
    18061 aaaaacaaaa gaaaacgcta accaacgatc atttttagct atttctcatc tttaaaatca
    18121 agcatttttt gccggtataa aatcgtaggg agaactgttt tccataccaa tcatcggatc
    18181 tactaactgg ttatcgatca cttctgctgt aagaacgaaa tctttagaaa cagcaggttc
    18241 tacctcttct ttcttttctt ctccaacaaa tgaagaaaac agatctggtt ccactgtttc
    18301 cattacttgg tttttcgttt cattcgtccc actgtcatct tctacaggta gcttttcttt
    18361 tacaacccgt ggctcttttg tatagttccc atcttcaaat ggatcatttc cgaccaaaga
    18421 atcttcataa gctgctagtt tggtccggag agtgatattc attttatcat tatccatgat
    18481 ccttgatttc aacgtagtca tccggcttgc tgattcattc gctacacaag tgacataggc
    18541 ttgctcttcc cctaacaaaa tcaacagttc cttactgcga gtcactgccg tgtagagcaa
    18601 attacgctga agcatccgct gatattgatg gaccatgggc aaaataacca tcttgaattc
    18661 actgccttgt gatttatgga ttgagcaaca ataagaaaga gtgattttat tccattcgtt
    18721 tcttttataa gtgacctcat ttgcatcaaa ctggataact aattcatcca ctttatcttc
    18781 agaatctttt gccaagatga tacccgtgat ttctcccata tctccattga atacattcaa
    18841 ttcgggagta ttgactaatt ggaggacttt gtctcctatc cgataaacgg tgtcgttcca
    18901 atgtacttcc ttttttccag gtttccctgg attgaatatc tcctgcatca ttttatttaa
    18961 cgcatcgatc cctgcaggtc cccgatacat tggtgctaat acttggatat cttgtgcacg
    19021 gaatccctta tttttcgctt tttccacaat tttagagatc aagggttcaa tttgataagc
    19081 atcacttcgg aaataggaac gatctttttg attctttgtg aaatcttttg gcagacgtcc
    19141 ttctttgatc tcatgggcaa gaggaataat actagaacca tcaccttgcc gataaatctg
    19201 attgagttcc attttgggaa tttgttcgat ttccaaaaga tcatggagta cttgaccagg
    19261 accgacagag ggaagctgat ctttgtctcc tactaagata acttgcatat tcgtcggaat
    19321 cgctttgaat aatgtgttag ccagccatgt atcgaccata gacatctcat caacaatcaa
    19381 aagtccgcct tctaattctt tcgtactgac tgaaggtgtt ttttcccgtc cattcagccc
    19441 cagaagacga tggatcgtac tggcaggcag acctgtcgtt tcgttcattc gtttagctgc
    19501 tcggccagtt ggtgctgcaa gtagaatagg aaaaatttct tgggtatatt ttgaaggatc
    19561 caattccagt ccgttcaact ctgcaaaaag attgacgatt ccgttgatca cagtagtttt
    19621 ccccgtacct ggtcctcctg ttaaaataaa tagaggggaa cggattgctt cagcgatagc
    19681 ttgttcttgc gaaggtccat actgtatgcc gaatattttt tcgatacgcc gaatattctt
    19741 ctgcacttct ttttcaggat aatggatttc ttttttacga ctgagaaggc gctggatcga
    19801 attaccgatc ccccattcag aaaagtacaa actgttttcg taaagattag tctcttcttg
    19861 ttgtatcttt ccttcttcaa caagttggat aattacagta gcaacctgat caggctggat
    19921 ttcaattggg cgacttgttt ctaaaatacg gatcgtttgc tgaagcaatg tctccgccgg
    19981 tacgtatgta tctcctgttt gaactgcatg ctgaaaaatc tgatgcaaga tagctgctcg
    20041 aattcttctc ggtgaatctg ctgcaatacc gatttgttcg gctagattat ctgcacgttt
    20101 gaacccgatt ccttctatgt cttcgactag ctggtacgga ttttcttcga tgatatccaa
    20161 agactcgttc ttataggcct ggtagatact gaaagctaac tgactaccga acccgtaacg
    20221 attcagtccc atgatcactt gatccatccc gtggtttagc cgaatcgtct cagcaagcat
    20281 ttctcttttt ggcttcgtca aaccggagat ttgttttaat accgaaggat cagcaatgat
    20341 tttttcgata gcatcttctc ccagcaattc tacaattttt tctgctgttt tcttcccgat
    20401 tcctgggaat ttttcgctgg aaagatacgc tacgatccca tttgcagttg ttggcttttc
    20461 ttgctcatat ctttcgagtt taagctgcct tccgtattta ggatgatcca ctaattctcc
    20521 ataaaaacga taagtctcat tttcttgaat atcgccgaaa cttccggtga ctacaatctc
    20581 tttttcacgg taatcagctg tggtttcact gatgtgtacc aaaagcactt tgtaaaaatt
    20641 actaggattt tgaaaaaaaa tcgcggcaac tgtaccaacg aaataaggca tttcttctgg
    20701 ctgcatcgga agccgcctcc ttcttctata gtaaaggttc tggtgctgtt tcagttccta
    20761 aaaaatctgt ttggttccat actttcagtt catatggcaa taatctgcaa ggctcttcag
    20821 tctctaaaat cgtcaaacta ctattgaaca atccacccat ttcacgcaac tcacttagct
    20881 cttttcctgt catccattga atggctgcag ttaaagaagc tccatgtcct acgaataaaa
    20941 gtgggccatc tttatgtcgg gcaaccgcat ctgctacaac agtttcgata cgtgtgattg
    21001 ccgattcaat cgtttctccg tgaaaaggag aaggatcata cagatctaaa tgattacgca
    21061 aattcggcag attatccgga tatttctggt acatttcatc gatcgactga ccttctaaag
    21121 tacccagccc tagttctctt agcttatctg tataaaaaat ctctaccgga cgagcaagct
    21181 ctttgttgat accttctgct gtatcacgtg ctcgctttgc tgtactggaa taaattgctt
    21241 taaaaggaac atattttacc tttttcccaa atgctttgat ttcttcgtaa ctttgtggca
    21301 aaagcggaga atcgccattg ctcccttgaa aacgtctttc ctgattccat tgtgtcttcc
    21361 catgtcgggt aaaatatagt tccatacgca gcaaactcct gtctttcttt tattttactc
    21421 tataataaac tgtctaatcc agcttgtcat tttcatagtg atgcgtcaat tctaaattag
    21481 ggaatccttg ctggcgaagt gcttcataca ctatcattgc agctgtattc gataaattca
    21541 atgagcggac atgctcatca ttcataggga tgcgtaaaca cttttcttcg ttttcacgca
    21601 taaatgtttc tggcaaaccg gttgtttctt ttccaaataa aaagaagtgc tcttctccat
    21661 cagcaaaatt ttcgtcacta tatactttat gggcaaattt tgagatcaaa tgtagtttac
    21721 gattacctaa aaatgttaaa aaagcttcta aattactgtg gtaagtaata tttacatcat
    21781 tccaataatc caaccctgcc cgttttagat gtttgtcatc agttgaaaaa cctagtggct
    21841 cgattaaatg aagcggagta ttcgtggctg cacacgtacg ggcaatattt ccagtgtttg
    21901 cagggattaa tggttcaaat aaaacgatat gattcatcag gatcattcct ctcatattta
    21961 tgttcgtttc tattttctga caatttgatt tttgcataaa aaaaaacgta tcaactcggt
    22021 gtcagtcact gcgagtcgtt acgttagtga agctcttctc actaactttt atcagaacag
    22081 gaacctgata aaaaccaatg aaaaaacaac ttgttgctaa tataacaccc tcaacttttt
    22141 tttgcaacct ctactcatta aaaactgcta gaaaaacgtt ttctcttagt tccttttaat
    22201 aattctatgt caattgtaat cttcttcaaa ggattcttat ataagtctgc tttgattcgc
    22261 tcttctacct gccattctaa aggtgacatg gccaaaagat ctttttgatt ttcctctggt
    22321 atctcaaatt cataggttac acgctgacgt tcagttaatt tcattgcttc agcaaacttg
    22381 gctactacac gttcattgga ataactctgt ttttcaggtt gattcggata aaaagcctga
    22441 cgtagttcct ttaaataatt ttcttgtggc accaccttca acacttctcc atcttctgag
    22501 agtacacgtt ggaattcttg gtaatgagaa ggcgaaaaga tatttaaaac cgcgttgaat
    22561 gtttgatcag caaacggcaa attggtcaaa tcagctacac accaaaatgc ttcggcagat
    22621 tgttctgtag ctgcgtagac tccttctttt gaaatatcaa atccaacagc attttgaatc
    22681 tttttcaatt ttgaaagttc attcaagaag ctaccttctc cgcagccaac atctaatagc
    22741 gagacttctt caggtaaatg ctgcgcaata gtctcaataa cgggctgata catgccagat
    22801 tggatcattc tacgccttgg aacgaacatt ccttgatcgt attctgtttt aaccgcatgg
    22861 gataaaaaat aaagtgttcc ttttttggaa aggtcaaaac ggtgattatt tttgcatatc
    22921 aagccattat cagacttttt tacttcttct tggcaaaccg gacagcggaa cagctgatga
    22981 ttttctgcca aaaactggcg tcctcggtca atttttttta acatagttcc ccctcatttc
    23041 tgttttatct taccgttaga tgctcacttt ggcaactgct aaaaatatga tacaatgcct
    23101 agaagaaagt gaaagataaa ggagaagatt agatgatcaa agaattttgc gctgaaaact
    23161 acacatctat tcctctagca attgctagag gagccgatcg gatcgaattg tgtgacaact
    23221 tagcggtcgg tggcacaacc ccgagtactg gtgtgatcga agaaacgtta agttatgctg
    23281 gggaaaaaga tatccccgta atgacgatta ttcgtcctag agggggcgac ttcgtctata
    23341 acgatatcga attgaaaatc atgcatactg atttgattga agcaaagaaa ttagggacgg
    23401 atggcgtagt atttggttgt cttactcctt caggctggct agacgaagag gccttagaac
    23461 tattgatcga aacagcagaa gggttgcaga tcaccttcca tatggctttt gacagtattc
    23521 ctgaagatcg gcaattcgaa gcaatcgatt ggctggtaga acatggtgtt gaccgtatct
    23581 tgactcatgg cggtcctgct gacaaaacta ttgtagaaaa tttcccacgg cttaaagaat
    23641 tgattgccta tacagatggc cgcttaatca ttttgcctgg tggtggtgtc actgcagaga
    23701 atgcagcaca tgtagctaaa gaattgaacg taagcgaagt acacggaacc aaaatcgtgg
    23761 atcttcagtt ataatcttat aaacaaaaaa acatttgcaa caggcataat gcttattgca
    23821 aatgtttttg tttttttaca taaaatagcc cataaataaa gaagcaatat tgaaataaat
    23881 cagtacactc gtcaaatcgc tcaaagtcgt gataaaggga ccgcttgcta ctgctggatc
    23941 aaatcctaga cgatccatta gcatcgggat taaactacct gcaagattcg ccactgtgat
    24001 tgcaaatagc atagccatcc ctatgacaaa ccccaagata aaattatgtt tccaaaaacc
    24061 tacgattaga aaaatcgtga tccccgtaac agctcctgtg acaacgccgg tcaagacttc
    24121 agccaaaaca agtcgaacaa atccatgatc tttctcatca gaaatcgcaa gtcgacgaac
    24181 agccacagct agcgactggg ttccagcatt accggcagtt cctgtaatca gcgaaatgaa
    24241 gactgctaaa atactcgctt cacttaccaa gtcttcatag tgactgatta aagaagcggt
    24301 cgacatacct aaaaacaaca gagtgatcag ccaaggcaaa cgtcgagacg ctgcctttac
    24361 tggattttca ttaatttctt ccacattgac cccggcaagt cctgaataat cgctagcagc
    24421 ttcatcatca ataacgtcga tgatatcatc gactgtgacg attcctaaca aatgatcatc
    24481 ataatcggta acaggaagag ctaagaagtc ataatctcta aatgtttgtg ccacatcttc
    24541 ttggtcgtcg cctacatgaa ctgaaagcac tcgttcgctc atcagatcag aaatcattgc
    24601 gtcgtcatca ttcacgatta ggtcgcgcag agaaataacc cctactagct gaccttcttg
    24661 attaacgaca tagatgtaat agattgtttc agctacatct gcttcgttct tcagcacgta
    24721 catcgcagag cgaacagttt gattggctac gatcgaaaca aattcagttg tcatgatcgc
    24781 gccagccgtt tcatcctcgt aatggagaag ttccttgatt tcactggcat cgtctgccga
    24841 catcaaactt aaatatttag caatttgctt tttatccagc gtattaagca agtcaacagc
    24901 attatctgta tacatctcag aaagcatatc agctgcatag ctaggacgca tttcatctag
    24961 gtattctttc atatgttcgt tgtcttcttc aatcacatcg aacatatccg ctaattcttt
    25021 aggcgacaga taggaataga tcagctgcct gttctcttct gtaagagatt ggtagaattg
    25081 cccctgctca tagatatgca tctctaaaaa actgtttctg aatgcctgaa tattattttc
    25141 ccttaaatct tgagtcaact gggcaaatct ttcctctaat tcttgatttt catccaaacc
    25201 aatctcacct cgccttattc ttcaaaccgt ctcacaactg cctccatgtc tgcggctaaa
    25261 ggctgcttga actccagcaa ctcttctgta aatggatggt aaaaacgcag atcataacaa
    25321 tgaagggctt gtctttttat tcccagatcc attcgcccgc catacatatc atcaccgagc
    25381 agggcacagc caattgcgga aaaatgaact cttatttgat gtgtccttcc tgtatgcagc
    25441 tggatatcaa ctaacgcgat ttctgttagt cgcttctcta tccagtactc agtctgtgcc
    25501 gaacgtcctt catcagagac cattcgcttc aatagcgaag aataatctcg ggcgatcggc
    25561 aaatcgatat gtccatgtgc cgataaatct gaaagccttc cactgactaa ggcctgatat
    25621 tttttcacaa acttcttttc acgaagttgc atatctaatt ttgcgtgcgc aaatccatgc
    25681 tttgcaaaaa gcatcaagcc agatgtatcc cgatctagtc gcgtcaccac atggattact
    25741 tggttttcgt aaccttgttt tttataataa gcctttacac gattagccat tgtcccattt
    25801 ggatggtatt gggcaggaat agacgaaacg cctgctggtt tatttactac cagaacgtgt
    25861 tcatcttcat acacgatatc gatcggtgtt tcatccaaaa gtacggtttc atgttctcct
    25921 tcagcaggaa tcacaatggt taccttatca tccttagcta gagaaaacaa gacattttct
    25981 acttgatcat taactttgat ttgaccgcct tgaaatttta ttttcgctaa cagacccttt
    26041 gagatccctt tttcttttaa aaaatatttt acttgctgag aatgatcttt ctcataatcc
    26101 catgtaaact gcatatcaat cctcactaat aaaggcatct ttcacacgat gccaaaaatg
    26161 catatgtcga taagaagcaa aatggatccg ttcttctgct attcgataat aaattgagcg
    26221 gatatttgct tttgcgacat tcaattgatc aaccgttaca agataatctt ctgtattttc
    26281 caattttatt tctagccatt catcatgggc aatcacgatc ggagaaccca aagtacgaaa
    26341 cactcgatta ttcagtgaag caatctctgc caactgaaag gcattgatac taggatggat
    26401 cacagcaccg ccgacacttt tattatatgc tgtcgaacca gtaggcgtag aaatcgtcaa
    26461 tccatctcct ctaaaacttt caaacagctc gtttttgatg tatacatcgg ctaccatcgt
    26521 acgattggcc cgtttaatcg ttgattcatt cagtgctaaa aaatgctggt ccggtgtttc
    26581 atccaaataa ctgatccgaa catccagcaa gggataacta acactttgtt cccggttcgt
    26641 tcgtaagctg tctactagtt cttccagctc gtaatcccgc caatccgtgt aaaaccctaa
    26701 atgacctgta tgcacgccca agaaacgcac atcattcaga caatgactgt agtgatggaa
    26761 cgccgacaat aatgtgccgt ctccgccgac tgagatgacc aattctggct catgatcatc
    26821 tcgttgaatc cctgcttgtt ctaaaagtgc gttcaaacgt tctgtcactt ctatagtctt
    26881 tgcttcttta ttatgaacga tcgctacttt catcccctgc ctcctctctt tcgctttctt
    26941 tcttcaaaag cgttcgatag tagacatcat ctgattttcc tcttccatgg gagaataacc
    27001 gctgtgcttc ttggatttct tcacggatac tggacatctc ttcatccaac tgataggcag
    27061 cttctgccgc acgctgaagt cgttcactca tttcttctgg aaaaacgcct tgatatttat
    27121 aattcagcga atgctcaatc gttgcccaga aattcatcgc aagagtccga atctggattt
    27181 ccgccaaaat tttcttctca ccgctgatca gctgtacagg atactcgatc accagatgat
    27241 aagaacggta gccacttgct tttttatttg tgatgtaatc tcgttcttgg agaactttca
    27301 tgtcttttct tttacggatg atctcaacaa cttgatggat atcttctaca aactgacaca
    27361 tgatccttaa cccggcaata tcagacattt cttcttctaa acgtcccatt ggaatatgcc
    27421 gtaattgtga ttttgttaca atactatcga ctggcttgac tctcccagtg acaaattcaa
    27481 tcgggacatg tcgattttgt tcacggaact gtttgcggat cccccgcagt ttcactttca
    27541 attctgatac agtttgttca tatggtgcta aaaatgcttc ccaatctcgt tccatagctg
    27601 atacctctac ttcattaaca ttcattgttt attctaccat atatgcaaag ttttttcgta
    27661 ctcttgattt atgcattgac caaaaaaaga aaattaggca aaatagacaa aaaggagtcg
    27721 atcaaatgac acaaatgctt gaaatcgaat ataaatctat gctgacaaag caagagtacg
    27781 agatattcat cgctcactat caattaacag ataaagattt ccgtgtacag actaatattt
    27841 atttcgatac cgttgatgaa cagctgaaga aacaaaattg tggcctacgg atccgtttcg
    27901 ttggtcatca agctgaatat acattaaaaa cacctgctga aaaagggcgt ctcgaaacaa
    27961 cggatacctt tataacagaa gaagcagaag catttatctt cgaaaaacgt ctgcctcgtt
    28021 caggcgcagt ttttcaaaaa ctttctgatt tgaatatcga tcctgcttca ttaatccaaa
    28081 ccggaaagct tacaacaaaa cgagctgaat tccctattga agaaggactt ttagctattg
    28141 atgaaagctg gggagacaat ctacacgatt ttgaactgga actagaggta ggggatgcat
    28201 ctgtgggaaa aattgctttt ataaattttt taaaacgttt ttctttacct tatcgtcctg
    28261 ctaagaacaa gatccaacgt atgcttgaag ctaaaaatta gaaacctttc attttcacaa
    28321 ggcacttgag ttcatttttt tacttttttc tgttaagtta taggatgtga aagaatctag
    28381 tcactagttg tatagaatct attttggggg agattcatat gatcgaaatc tatttatttg
    28441 ttaatccttt aggggaatac tgtttcgaaa tggaacaaca gttattacaa tttatcgaag
    28501 aagagtacgg gacaccatca aaagaaaaga tgcaatttcg ctttcttcct ttggtcaatt
    28561 tgcagacaat tggggatgtc atgcaacaaa aaggaatttc tcagaacaat cttgaggaac
    28621 gtaatcactt attctcaatg acttattctg cagcattgga ctgcaaagcg gcgcagtttc
    28681 aaggaaagaa aaaaggccgc cgttttttga tcaaattgca agaagcagtc ggctgtaatg
    28741 acataccata ttctcaagaa cttgctgaat ccatatttga agaaatcggc ggcgatttga
    28801 atatgtttaa agaagatcgt cagtctgact tcgttaaaga agtattcttt tctgatcaaa
    28861 aaattgctcg ggaaatgggc atcacaaaac acccttctgc agtcgtttac aattatgcct
    28921 gcgaccgtga ttttggtgtt ttagtagaag taaaaggtac aagtagcctt tggagaatca
    28981 cagagctgtg taaaacaaca gataactcat atcaagtttt taatggtgaa catttacata
    29041 caaatgattc tcagttccat tcaagaacta gccatttaca cttgctttct aattaaaaa
//
LOCUS       DASHHB010000032        28033 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.033, whole genome shotgun sequence.
ACCESSION   DASHHB010000032 DASHHB010000000
VERSION     DASHHB010000032.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28033)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28033)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28033
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.033"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            185..1183
                     /locus_tag="U2K71_001612"
     CDS             185..1183
                     /locus_tag="U2K71_001612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM7315696.1"
                     /translation="MEIYDITIIGAGPVGMFAAFYAGMRQAKTKIIDSLPQLGGQLAT
                     LYPEKYIYDIPGYPAIKASELIDQLEKQLTTFNHTFHLKEEVLSLTREDEVIEITTNK
                     GIHYSKAVILALGNGSFQPRKLNLDNAESFENHGLDYFVNDLMKYAGKKVAIAGGGDS
                     AIDWTLMLEPIASEVYLIHRRPEFRAHEHSVSRLKSSSVNLLTPYLIDGLSGNNGELT
                     DIRLKKVKSDETIDLMIDSLIVNYGFSSNLEHLSSWGLDSTRNAITVKSDMSTSIPGV
                     YAAGDICTYEGKVKLIATGLGEAPTAVNNALHYINPKERTQPGHSTSLYDKNLRPS"
     gene            1295..1804
                     /locus_tag="U2K71_001613"
     CDS             1295..1804
                     /locus_tag="U2K71_001613"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylglycerophosphatase A"
                     /protein_id="HEM7315697.1"
                     /translation="MVVKTETLEEKARELLKERGVTVEDIAQLVMFLQKDYISDLTLA
                     DCTESVNSVLTKREVHNAIITGIQLDILAEKKKLMSPLQHIIEDDEGLYGIDEILALS
                     IVNVYGTIGFTNYGYIDKVKPGILKELNSHDGVHVHTFLDDIVGAIAAAAASRLAHKE
                     PEKRSRLTQ"
     gene            complement(1834..2493)
                     /locus_tag="U2K71_001614"
     CDS             complement(1834..2493)
                     /locus_tag="U2K71_001614"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HEM7315698.1"
                     /translation="MKNSRWQWMEYAGLFSLFLTLISLAVGVTINFRPLYVFDIGHLQ
                     ILDYTSLDQETLLKNFDHLMNYLNNPFKTILSLPDFPVSASGAHHFYEVKILFLVDYA
                     VFFITLIPSILFIKYLQKNDRLWRLIRPFQIGMLLPVVFGFFMMIGFDRFFILFHETF
                     FNNDDWLFDPVTDPIINVLPEEFFMHSFILFFVLLELFFAVFLFLGKNSLKQTKKKEL
                     V"
     gene            complement(2495..3259)
                     /locus_tag="U2K71_001615"
     CDS             complement(2495..3259)
                     /locus_tag="U2K71_001615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01457 family HAD-type hydrolase"
                     /protein_id="HEM7315699.1"
                     /translation="MDYKGYLIDLDGTIYRGTQPIPAGKRFVEELQKRKLPFLFVTNN
                     TTKSPETVANRLADEFDIHVAPETVYTATLATIDFMKADGKGKKVYVIGEAGLIDLIL
                     AAGFTWEEEAPDYVVVGLDNYLTYEKVVKATLAIQKGATFIGTNPDKNIPTERGLLPG
                     AGSVISFVETATQTPPIYIGKPEAIIMDKAVEVLGLQKEEVIMVGDNYETDIQAGIRN
                     NIDTLLVLSGFTKEEDVPGLPIPATYVKQSLDEWSF"
     gene            complement(3267..3944)
                     /locus_tag="U2K71_001616"
     CDS             complement(3267..3944)
                     /locus_tag="U2K71_001616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YutD family protein"
                     /protein_id="HEM7315700.1"
                     /translation="MSAKQNEQQNVEHKTEIAKKKSSAEQETTVTEELTAALEEIVEE
                     PKAEKVKGELVTLLDDSNFLIGERHYRLVSDYREGFNAEKLGERYSDVLARYDYIVGD
                     WGYEQLRLKGFFQADNRRAHPDQRIDSLEDYLYEYCNFGCAYFVIERIGGKKEKTTQR
                     RKKKKNHNRAYIDEKKGPVASNRKKPVIKNRKTESAKTSEKVKKQEQPKQVEKTKTAF
                     TIRKREE"
     gene            complement(3981..5366)
                     /locus_tag="U2K71_001617"
     CDS             complement(3981..5366)
                     /locus_tag="U2K71_001617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEM7315701.1"
                     /translation="MEKIVILHTNDLHSHLENWPRIRRFLDQRKRENEKKEKTTTIAV
                     DLGDFVDRWHPLSEATNGQENILLMNEVGYDAATIGNNEGVGNSKEELNHLYDHAEFD
                     IILDNLFDKNTLQPPAWTKPYKIITTKHQTKIGLIAFTAPFPLTYNPNGWDIRNPYDI
                     LPELVEDLRSQVDVLVLMSHLGIQDDFQIAKEIPAIDVILGSHTHHLFREGHVINDVQ
                     IAAAGKYGQYVGEVHLTIDEEKKILKHSAKAIPTETMTAFTEDEQEINSYLERGHELL
                     KEKEVADLPFSLSLDIFNEHSFIQVAFEAVKERGKTEAAILNSGLFLTEIPKGRVNQD
                     QLHTALPHPMHLLNVTLGGNDLIRLVLEIEKNRNFLRNYPMKGMGFRGKIFGQMVYNG
                     ITYDSINHQVFWKNKPIDLNKNYTFTTVDHLMFVPFFPTIEIAGKNEFLFPEFIRSVV
                     GDYLKAHYPIK"
     gene            complement(5576..5660)
                     /gene="ffs"
                     /locus_tag="U2K71_001618"
     ncRNA           complement(5576..5660)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="U2K71_001618"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="U2K71_001619"
     CDS             complement(5683..6198)
                     /gene="tadA"
                     /locus_tag="U2K71_001619"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA adenosine(34) deaminase TadA"
                     /protein_id="HEM7315702.1"
                     /translation="MVNTNLDAVEKEKWMRLALAEAKKAEMLHEVPIGAVVVLDGKVI
                     GRGYNLRETTQDATTHAEMLAIKEACEKVGSWRLEDAALFVTLEPCPMCSGAMILSRV
                     AEVYFGAYDPKGGTAGTLMNLLEDERFNHQAYVEGGILEDECGMILTDFFRKLRQRKK
                     KLKKDWSTNEK"
     gene            6305..7162
                     /locus_tag="U2K71_001620"
     CDS             6305..7162
                     /locus_tag="U2K71_001620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315703.1"
                     /translation="MKMDKLLALYPQAQKKNSPASEADILSFAIDDAFIWIKQDSLSQ
                     QETSLLKALFPVINDQKKHPWYHYLFKDASCVQEKSFRLIQLHVESKGEFLKKEWQDT
                     ILELFRYSADFFFYTDTDALLVEQINRDYLDATEINGIFLSLDADFDTTTSVFVGSFH
                     SPGTDLVPLFAEERKIFLTEQNNLYTHSRTFSMQDVALHYYTKDTVKDSKLIKSYIEL
                     IKQNEMEVIIQELWNELGNVSSAAKTLFLHRNTLKYRIEKFQEQSGFNLKKANDLLFC
                     YLLLLHEHN"
     gene            complement(7243..7578)
                     /locus_tag="U2K71_001621"
     CDS             complement(7243..7578)
                     /locus_tag="U2K71_001621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315704.1"
                     /translation="MKTSTKVTIGLSVAAAASVATAVVVSGKVMEKIHHMTTRTKVKK
                     FVYDKFDGNEKLLEIVDGLSDSDLDSLMGMLAKIKSGKKKISVYGDSIKDSTEDVKDR
                     LMHFVEKMM"
     gene            complement(7645..9015)
                     /locus_tag="U2K71_001622"
     CDS             complement(7645..9015)
                     /locus_tag="U2K71_001622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HEM7315705.1"
                     /translation="MKEEATTLPQQFIKKYRLLLGEEASDFFSALEQGSVKKGFRWNP
                     LKPAGLDMVQTYHSEELQPAPYSNEGFLGTVNGKSFLHQAGYEYSQEPSAMIVGTAAA
                     AKPGEKVLDLCAAPGGKSTQLAAQMKGKGLLVTNEIFPKRAKILSENIERWGVSNAIV
                     TNHAPAELVPHFSGFFDRIVVDAPCSGEGMFRKDPNAIKEWTEESPLYCQKRQQEILS
                     SAIKMLKNKGQLIYSTCTFAPEENEEIISWLVENYPVTIEEIPLTQSVSSGRSEWGSV
                     AGLEKTIRIWPHKDQGEGHFVAKLTFHGQNQMHKEKKTRKKSKVQMTKEQEKLWTEFS
                     NDFHYEATGRLLVFNDHLWEVPELAPSLDGLKVVRTGLHLGDFKKNRFEPSYALALAT
                     KKIENIPCLPITQKEWQSYTAGETFQRDGNQGWVLLVLDKIPVGFGKQVKGTVKNFFP
                     KGLRFH"
     gene            complement(9093..9629)
                     /locus_tag="U2K71_001623"
     CDS             complement(9093..9629)
                     /locus_tag="U2K71_001623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7315706.1"
                     /translation="MMGRVTNYSKEYLLFKSMVYVEEYGMKSITARELAEFCGCSTYP
                     IYTHFKSISGLKEKILEEITVYFENYLAECSSDDVLSIVYLLKDFFSIHESIREIIAK
                     SDLDMDSLFKRPFFSYVKGHTKIKEEKSLELVWLNALGSLRSDDSSQDFLEMMNAVWD
                     KNEVTDEYLENILKEQSP"
     gene            9938..11419
                     /locus_tag="U2K71_001624"
     CDS             9938..11419
                     /locus_tag="U2K71_001624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315707.1"
                     /translation="MLIEARKKELLVLQMVIQKNNCSLQELSNDLHIPKRTIKELIRK
                     INVSLKQFLLIDEFIYSNHKGEIQTKETDQTKKLAVFSKLKLCYLKESNRFNYLMLLI
                     NYPQTAVPKKYLLEQLYISPSYLEKLTKQLNHSLIKFHLRILSVKNCCTLEGNELSIR
                     LYLYFFLSDTFGGNDWPFSSLELNALKMNSTDEQIEKISKQEKNIYLLLTLFILRNKQ
                     QAYFPKTSSEITELMDLLQHTHDYSEYFTSFSVSRPCEETETERAYFNYFFYCFFPNQ
                     ISFRQKQEIGFLFSTSKNLICRQVGQLMTYLTQQFPLNHLENKIPIYFYFLVLAVVFF
                     ELLGESSSSFSTLCFPEATSNHSSSFIQEKKLREKIDTIFSKNGERNQISQYLSQLIQ
                     HLLQTETKEQLLIHLQITKQFTGNYHLQSHLQQLFRKDAVKITAYSEHADLIITDQHE
                     KISDETETFYLDTTHNTVLFGKLIKAVHELYMKKGYENKTKRM"
     gene            complement(11522..12229)
                     /locus_tag="U2K71_001625"
     CDS             complement(11522..12229)
                     /locus_tag="U2K71_001625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc metallopeptidase"
                     /protein_id="HEM7315708.1"
                     /translation="MYGYGMYGFLDPTFLLVILGLVISGIASAYVNSTFRKYDQVRSK
                     NNVTGTQAAQYILQSQQINNVGVQQIAGDLTDNYNSGNKMLSLSQATAQSTSVAAIGV
                     AAHECGHAVQDASGYVPLKIRAALVPAANFGSMISFPLIMVGVLLSWNSTLINIGIFA
                     FSLALLFQLVTLPVEFNASRRAIQILSEGGLLTEEEVPMARHVLFAAALTYVAAALST
                     FLQLLRLILLFGGNRRD"
     gene            complement(12308..12646)
                     /locus_tag="U2K71_001626"
     CDS             complement(12308..12646)
                     /locus_tag="U2K71_001626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkylphosphonate utilization protein"
                     /protein_id="HEM7315709.1"
                     /translation="MTLPNCPECGSEYTYEDRGLLICPECGNEWNKEEQETVIEDGLV
                     VLDANGNRLLEGDSVTVIKDLKVKGASGAIKQGTKVKNIRLVEGDHNIDCKVEGFGPM
                     KLKSEFVKKN"
     gene            complement(12757..13719)
                     /locus_tag="U2K71_001627"
     CDS             complement(12757..13719)
                     /locus_tag="U2K71_001627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HEM7315710.1"
                     /translation="MTVQQEIVDTIKKYDKIIIHRHLRPDPDALGSQVGLAELLRESF
                     PEKEIRQVGETIEGLRFLAEMQEVEDVFYQGALVIVTDTANSPRISDQRYQLAEKLIK
                     IDHHPNDDPYGDLVWVNTKASSCSEMIAEFALMFPESLKMNVKAARLLYAGIVGDTGR
                     FLYPSTTSRTLEIASILRNYPFDASALNREIEQMPMKVAKLSGYLYQNIQVDENGAGR
                     IILPQEILDKYGIDDSETASIVSLPGVIDEVLAWGIFVQQPSGYYRVRLRSKGPIINE
                     LAKRHHGGGHPLASGANAKDLAEVEEIYKEIQDICQSYKSRKTE"
     gene            complement(13734..15065)
                     /locus_tag="U2K71_001628"
     CDS             complement(13734..15065)
                     /locus_tag="U2K71_001628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM7315711.1"
                     /translation="MATKHDLILEYIESLPVGNRISVRSIAKELNVSEGTAYRAIKDA
                     ENVGLVSTIQRVGTIRIERKLKKHIERLTFGEVVRIIEGDVLGGAAGLDKVLNKFVIG
                     AMTKHAMERYITPGSLMIVGNRAEVQKLALEDGAAVLITGGFNTTAEIAKLADDLEMP
                     ILRTSYDTFTVATMINRALSDQLIKKDIMLVSDIYMPAEKTHYLQQTDTISDYQRLSE
                     ETQHSRFPVVNRHHRLMGIVTAKDVLGKSPNQIIDRVMTKEPISVKKTMSIASVSHQM
                     IWDGLEVMPVVSDDLTLEGLITRQDVMKAMQLVQRQPQIADTISDQISGNIHTIDTDR
                     EGNRLENPKFKFVVAPQMVNGVGTISFGVLSEIISDVAQKTMVMNQKRNILIEQVNLH
                     YLRLIQLESELDIRPRVLEIGRRSAKLDIEVFIENTIVSKAIVVCQVMERS"
     gene            complement(15073..15753)
                     /locus_tag="U2K71_001629"
     CDS             complement(15073..15753)
                     /locus_tag="U2K71_001629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal-dependent hydrolase"
                     /protein_id="HEM7315712.1"
                     /translation="MKITYHGHSCLTIEMENGQRVMIDPFITGNPLTDLTAEEVKTDW
                     LLITHGHSDHIGDMIPIAKENQATIISIVEIADYANSRGVDSFGMNIGGKHAFPFGVV
                     KFVHAQHSSSYEVDGIVQYMGEPSGIIIQAEGKTIYHAGDTAYFSDLGLLAEEFDIDV
                     AFLPIGDNYTMGPEDAAKAAQTIRAKKVVPIHYNTFPVIEQDPESFVELLEDSEGKIM
                     SVSETLEV"
     gene            complement(15972..16451)
                     /locus_tag="U2K71_001630"
     CDS             complement(15972..16451)
                     /locus_tag="U2K71_001630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HEM7315713.1"
                     /translation="MMSQTKVERYLEEQKISYQPLDFSHVVEESFTEELQKRGIDPAL
                     ICKTLVVKGNKTGVIIALIPLNDHLDYKKTRQVTKDRKVGFPGMDFVLMHTGYPHGAN
                     TPIGIKLAHPDYCFLADSSLKEKEEVIVSSGEIGRSLRIKVADLEKIVHPIYADLIS"
     gene            complement(16578..18257)
                     /locus_tag="U2K71_001631"
     CDS             complement(16578..18257)
                     /locus_tag="U2K71_001631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEM7315714.1"
                     /translation="MSTIKIIPLGGVRENGKNMYVAEVGEDIFILDCGLKYPENELLG
                     IDVVIPDFTYLEENIDRVAGVFLTHGHADAIGALPYLLSKISVPVFGTELTIELAKLN
                     VSRNEAAKGFKDFHVVDEHTEIDFGETVVSFFRTTHTIPDSVGINLKTKEGSIVYTGD
                     FKFDQTAIPMYQTDYARLAEIGKEGVLALLSESANAENPQPVASEAQIADEVFDTIKY
                     WEGRIIVACVASNLQRVQQVLDAAEKAQRKVVLTGQDFERIIRTAMRLEKLQLPSEDL
                     LVTLKDMKNYDPEELIILETGRMGEPIKSLQKMANGTHRNVKIEEGDLVYITTTPSIA
                     METIVAKTEDIIYRAGGTVKLISENMRVSGHANPNDLQLMINLMKPTYFVPVQGEYRE
                     LAAHADLAHAVGMPYKNIYITGRGDVLEYKNKRMSVAGTTSAENVMIDGLGIGDIGNI
                     VLRDRKILSEDGIFVAVVTISRREKKIISKPQITSRGFVYVKASRDLMKESSNMIETI
                     VEKHLESNDFEWSKLKQDIRDQLGRYLFEQTKRRPVILPVIMEATQRRNKK"
     gene            18805..20307
                     /locus_tag="U2K71_001632"
     CDS             18805..20307
                     /locus_tag="U2K71_001632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7315715.1"
                     /translation="MNFMKRAWKSTKVKWGRSLLLFAVFTAILIFVLAGLTIRSAAEE
                     AANQAQKEVGATVTLSANREASFKKQEQTSSSGSTTEERPDPGSFSLTPVSLTDAEKI
                     AELDNVKSYSFESSASAMAKDGITAITSSDSSEDSSETTDAGGEPAGGMGGNPQMTQA
                     DFQISGVSATAQNSNFSDGTAKITDGEGIDDSDKGTNNVVIDSTLAETNDLAVGDTFT
                     ITSTEDEDTTYEMTIKGIYESSETSGSMGMKFNFMNPSNTLYTSYTFANELNGTSDDN
                     TIDSAIYTLSDPDKMDEFLKEAEKLIDTDTFSLQSNDSMYQSMLEPLNNVASFSKNVV
                     LLVAVAGIIILTLIIMITIRERRHELGVLLSLGESRSKIILQLFTEVAICMILALGVA
                     SLSGNVVANAVGQQLLDQQTETTTQNQQAPGGGQMPGNRQEQGGQRDGQQGTTNNPFA
                     VSDQVSELEINVKPAQLGLLAGVAFGISLFSVFLASIGILRLNPRKILLN"
     gene            20320..20982
                     /locus_tag="U2K71_001633"
     CDS             20320..20982
                     /locus_tag="U2K71_001633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315716.1"
                     /translation="MLRTEQLGFWYQDPQDALYKDVELNFEAGKMYAILGASGSGKTT
                     FLSLISGLDAPKEGKVYYQDRSLNKIGLRSYRKNDVSIIFQAYNLLPYLSALDNVIAA
                     MEITKTKQENYRQVALDRLARVGIDEILAKKQVTKLSGGQQQRVAIVRAICCEHQLIV
                     ADEPTGNLDETTSQDIVKLFQEIAHEQNRCIIIVTHEIEVAKSCDEVFELKNKKFQKI
                     EV"
     gene            complement(21083..21286)
                     /locus_tag="U2K71_001634"
     CDS             complement(21083..21286)
                     /locus_tag="U2K71_001634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEM7315717.1"
                     /translation="MADLKGRSKDMKDKVVGEGKEAYGKATDDKSKETEGKAQSLGAD
                     IKEKARNLGDEVKDKFSRKDEKK"
     gene            complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="U2K71_001635"
     CDS             complement(21397..22581)
                     /gene="iadA"
                     /locus_tag="U2K71_001635"
                     /EC_number="3.4.19.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-aspartyl-peptidase"
                     /protein_id="HEM7315718.1"
                     /translation="MKIIRQIEVFAPDYLGKMDVLIAGGKILAVEEQLEGGYEGVEVE
                     ELSGEGKVLTPGFIDCHFHILGGGGEGGYQNRTPEVTLSQLTTAGVTTVVGCLGTDGE
                     GRDMTALISKAKGLEAEGISTYVYEGSYRLPVKTVTDSIIKDFLTIDKIIGIGEIAVS
                     DHRSSQPSFEEFARAVADARVGGMLSGKAGIINVHLGGGKRKLELLTRIVEETEIPIT
                     QFLPTHANRTPELFEACVAFAKRGGTIDFTASEDPDFWEKTDGEVRFSKALKRLIEED
                     VSLDNFTMTSDGQGSLPYFDENNHFLGLGVGSAKALLVGIKEAVQKESIPLEIALRAI
                     TSNPARILKLDKKGKIEIGADADLCILDKETLDIDTVIAKGEIMVQEKEVKVWGTFEK
                     SF"
     gene            complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="U2K71_001636"
     CDS             complement(22738..24477)
                     /gene="yfcC"
                     /locus_tag="U2K71_001636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEM7315719.1"
                     /translation="MKKTNSSGKPKRDFTKLSTPHTYVIIFGVVIFAWILTFVVPAGK
                     FSTQDIEYKDANGETSTRTVLRQDSFRYAYELDKSYVFDQLEELQDHPAEREKLDVPE
                     KGLEKVIADGEKNLTQEKLDEISLTDDVLYDQYGENIYDTSKKLHKTAKIWGTDDFGG
                     FGFLNFVFEGLVSGDKYGSAVGIAALILVVGGAFGIIMRTGAIDAGIYAFISKTKGLE
                     RLALPLLFFAFSFGGATFGMAEEVIPFSMVMVPFVIALGYDSIVAVTVTYVASQVGNA
                     TSWMSPFSVAVAQGIAGIPVLSGATFRLIMWVVVTALAAGYMMIYAEKIRKKPESSLT
                     YKSDDYFRSHIKKTSDENKKFMLGHKLILLEMLVVLVWIVWGVTQKGYYIPEIASQFF
                     VMGLVAGIIAVLFKLDGMKVNDIASSFQSGAADLAGTAIVVGMAKGILLVLGGSDASV
                     PSALNTILHGIGTALTGVPAVIGAWAMYIFQSLFNLVVTSNSGQAALTMPIMAPLADL
                     VGVSRQVAVLAYQLGSGFMDAFTPVSASLIGVLGVARIEWAKWARFQIKMQGFLFVLG
                     TIFIMIAIAIGLQ"
     gene            complement(25250..25864)
                     /locus_tag="U2K71_001637"
     CDS             complement(25250..25864)
                     /locus_tag="U2K71_001637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM7315720.1"
                     /translation="MSLCYFDYYHQLEEKLRIEEPLMLQAGTWIAKQINQGGRLQIYA
                     SRTLSGVAFEFWDQTPDLVPSILIENPAGGVYETLEGAGQAIIDQLSVKSEDIFLLLS
                     NEGRNPAIIELAQWIKENGHPLIIVTGFDLSRSIKSQHSSGLRLFEFADLVLDNHANL
                     HDAVLTLPNLDPAICGTASLATIFLLQQVLYFTAKQLVQDTSGK"
     gene            complement(26470..26847)
                     /locus_tag="U2K71_001638"
     CDS             complement(26470..26847)
                     /locus_tag="U2K71_001638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315721.1"
                     /translation="MGKLVAVAQSLLDILKWLMEGSNWIFVLPLLLISLFIMFRMVRK
                     KETFLIFIWYAASVGVATLMVELALGSWEACVFALFFAVLYLIIYIPYMKRKSRIGRK
                     GRHTTTFSSTDSLEQSRRTRRRR"
     gene            complement(27147..28028)
                     /gene="galU"
                     /locus_tag="U2K71_001639"
     CDS             complement(27147..28028)
                     /gene="galU"
                     /locus_tag="U2K71_001639"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEM7315722.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LDSGIEDILIVTGKEKRPIEDHFDANIELENNLREKEKTELLALVEETTQVNLHFIRQ
                     SHPRGLGDAVLQAKAFIGNEPFVVMLGDDLMKDDIPLTQQLMMDYEETQASAVAVMRV
                     PENETSKYGIIDPKTDSEKGRCRVKGFVEKPEMGKAPSNLAIIGRYLLTPKIFEILET
                     QEPGAGNEIQLTDALQTLNQTEAVYAREFKGKRYDVGDKLGYMKTNIEYGLQHSEIGT
                     SLSAYIIQLSKKLRTES"
BASE COUNT         8325 a         5874 c         4686 g         9148 t
ORIGIN      
        1 atttgcatac ttcataataa taataacata atacctaacc tctcgtaaaa atactagatt
       61 tgttttcttt tatttttcat cactagatac attctataac ttaccgtttc gatttcgaat
      121 tcaaacattt ttgagaagca ttctcaatta aattctgata tagtgtgaaa agggaggaat
      181 ttttatggaa atctatgaca ttacaatcat cggtgcaggt cctgtaggga tgtttgccgc
      241 tttttacgca ggaatgcgcc aagctaaaac aaaaatcatt gacagtctac ctcaacttgg
      301 tggtcaatta gcaactttgt atcccgaaaa atatatttac gatattcctg ggtatccagc
      361 aataaaagca agtgaattaa tcgatcaatt agaaaaacaa ttaactactt ttaaccatac
      421 attccattta aaagaagaag ttctctcgct tactcgtgaa gacgaagtta tcgagattac
      481 tacaaataaa ggcatccatt attccaaagc agtcatttta gctttaggaa atggttcatt
      541 ccagccaaga aaattgaatt tagataatgc tgaatcattt gaaaaccacg gattggatta
      601 tttcgtgaat gatttgatga agtatgctgg aaaaaaagtc gctattgctg gaggtggtga
      661 ttctgctatc gattggacgt tgatgttaga gcctattgca agcgaagttt acttgattca
      721 tcgtcgcccg gaatttcgtg cgcacgaaca tagtgtatca cgcttgaaat cttcatctgt
      781 caacttgctt actccctatc tcatcgatgg attatcaggc aataatgggg aactgacaga
      841 tatccggtta aaaaaagtga aaagtgacga gacgattgat ttgatgattg attctctgat
      901 cgtcaattat ggattctctt ctaatttaga acatctttct tcttggggac ttgatagtac
      961 cagaaatgcc attactgtaa aatcagatat gtctacttct attccaggcg tttatgcagc
     1021 tggtgacatc tgtacctatg aaggaaaagt gaaactgatt gctactgggc tcggtgaagc
     1081 acctactgca gtgaacaacg ctttacacta tattaatcca aaggaacgaa ctcagcctgg
     1141 tcacagcaca agcttatacg ataaaaatct tcgtcctagt tgatttttta tatctctttg
     1201 catttcaagt taaacccgaa agcagtttct tgcatttttt tcatgttccg ttaacatgaa
     1261 atagagaaag aaaaaataaa gggggagcaa taatatggtc gtcaaaactg agactttaga
     1321 agaaaaggca agagaactat tgaaggaacg tggcgtaact gtagaagata tcgcacaact
     1381 tgtcatgttt ttacaaaaag actatatttc tgatttgact ttggcggatt gtactgaaag
     1441 tgtcaacagc gtgctaacaa agcgtgaagt acataacgcc attatcactg gaatccaatt
     1501 agatatatta gcagaaaaga aaaaattgat gagtccgttg caacatatta tcgaagatga
     1561 cgaaggcctt tacggtatcg atgagatcct agccttgtca atcgtgaatg tttacggtac
     1621 gatcggcttt acgaattatg gttatatcga caaggtgaaa cctggtattt taaaagaact
     1681 aaacagccac gatggcgttc atgtccatac cttcttagat gatatcgtcg gagcgatcgc
     1741 cgctgctgct gccagtcgat tagcacacaa agaaccagaa aaaagaagtc gccttactca
     1801 ataattcaac aaaaaaagaa agaaaacgtc tatttagacg agttctttct tttttgtttg
     1861 tttcaagcta ttttttccta agaaaagaaa aacagcaaaa aataattcta ataatacaaa
     1921 aaataagata aagctatgca tgaaaaattc ttctggaagt acattgatga ttggatcagt
     1981 aacgggatcg aataaccagt catcgttgtt gaaaaacgtt tcatgaaaaa ggataaaaaa
     2041 gcgatcaaat cctatcatca tgaaaaaacc gaaaacaact ggcagtaaca tgccgatttg
     2101 aaacggacga atcaaccgcc aaagccgatc attcttttgc aaatatttga taaacaaaat
     2161 actcgggatc aaagtgatga aaaacacagc ataatccact aaaaacaaaa ttttcacttc
     2221 ataaaagtga tgggctccgc tagcagaaac tgggaaatct ggcaagctga ggatggtttt
     2281 gaagggatta ttgagataat tcattaggtg atcaaaattt ttcagtaacg tctcttgatc
     2341 taaagaagta taatccaaaa tttgcaaatg tccgatatca aatacgtata gtggtcgaaa
     2401 gttgatcgtc acaccaactg ccagactgat cagtgtcaga aataggctga acaatccagc
     2461 atattccatc cactgccatc tcgaattttt catattagaa gctccattca tccagtgatt
     2521 gtttcacata ggtagctggg ataggcaacc caggtacatc ttcttctttt gtaaaacctg
     2581 ataaaacgag caaagtatct atattattgc gtatcccagc ttggatatct gtttcatagt
     2641 tatctccaac catgattact tcttcttttt gcagccctaa tacttcaacg gctttatcca
     2701 taatgatggc ttctggtttt ccgatgtaga tcggcggcgt ttgggttgct gtctctacaa
     2761 aagagatgac tgaccctgct ccaggaagaa ggccgcgttc agtaggtata ttcttatctg
     2821 ggttcgttcc aatgaaagta gcaccttttt gaatagcaag tgttgcttta actacttttt
     2881 cataggtcag ataattatct aagcctacca ctacatagtc aggagcctct tcttcccatg
     2941 taaatccggc agctaaaatc aagtcaatca atcctgcttc tccgataaca tagacttttt
     3001 tcccttttcc atctgctttc ataaaatcga ttgttgccaa agtagctgtg tagactgtct
     3061 ctggagctac atgaatgtca aactcatctg ctagacgatt agctactgtt tctggtgact
     3121 tagtcgtgtt attggtaaca aacaagaaag gtaattttct tttttgcaat tcttccacaa
     3181 atcgtttacc ggccggtatg ggttgtgtcc cacgataaat cgttccgtct aaatcaatta
     3241 aatatccttt atagtccatt catccactac tcttcccttt ttctgatcgt aaatgctgtt
     3301 tttgtttttt ctacttgttt aggttgttct tgttttttta ctttttcaga ggtttttgct
     3361 gactctgttt ttcgattttt aataaccggt tttttgcgat tagatgcgac tggtcctttt
     3421 ttctcatcga tatacgcacg attatggttt tttttctttt ttcggcgctg agttgtcttt
     3481 tcttttttac cgccgattct ctcgatgaca aaatacgcac aaccaaaatt gcaatattcg
     3541 taaagatagt cttccaagga atcgattcgc tgatctggat gcgcccgtcg attatccgct
     3601 tggaaaaatc cttttaggcg tagttgttca taaccccaat ctcctacgat ataatcatat
     3661 cgggctagta catcactgta acgttctcca agcttttctg cattaaatcc ttcacgataa
     3721 tcggacacaa gccgataatg tctttcacct atcaaaaaat tactgtcatc taacagtgtc
     3781 actagctcgc ctttgacttt ttctgcttta ggctcctcaa cgatttcttc caaagcagct
     3841 gtcagttctt cggtgactgt tgtttcctgt tcagctgatg attttttctt agcaatttct
     3901 gttttatgct caacgttctg ctgttcattt tgttttgcag acatgcgttc accacctatc
     3961 ttttttatta taccttgttt ttatttgatt gggtagtgag ctttcaagta atctcctacc
     4021 acacttcgga taaattcagg gaacaaaaat tcattttttc cagcaatctc aatcgttgga
     4081 aaaaatggaa caaacatcaa atgatctacg gtagtaaaag tataattttt attcagatcg
     4141 attggcttgt tcttccaaaa gacttgatgg ttaatagaat catatgtgat tccattatag
     4201 accatctgcc caaaaatctt accgcgaaat cccattccct tcataggata gttacgtaaa
     4261 aagttacgat ttttttctat ttctaaaact aagcggatca gatcatttcc acccaatgtg
     4321 acatttaaca agtgcatcgg atgcggcaac gcagtgtgaa gctgatcctg attgacccgc
     4381 ccttttggaa tttcagttaa aaacagacca ctgtttaata ttgctgcttc cgtctttcct
     4441 cgttctttca ccgcctcaaa ggctacttgg ataaacgaat gctcattgaa gatatctaga
     4501 ctcagagaaa atggcaaatc tgctacttct ttttctttta ataattcatg cccacgttct
     4561 agataactat ttatttcttg ttcatcttct gtaaaagctg tcattgtttc ggtagggatg
     4621 gctttggctg aatgcttaag gatttttttc tcttcatcga tggttaaatg gacttcaccg
     4681 acatactgcc cgtactttcc agcggctgct atttggacat cattgattac gtgaccttct
     4741 ctgaataaat gatgggtgtg cgatcctaaa ataacatcaa ttgctggtat ttcttttgca
     4801 atttggaaat catcctgaat cccgagatga ctcatcaaaa ctaaaacatc cacttgtgaa
     4861 cgtaaatctt cgactaattc tggcaaaata tcataaggat tccgtatgtc ccagccattc
     4921 ggattataag taagcggaaa tggagcagta aatgcaatta gtccgatttt cgtctggtgt
     4981 ttcgttgtaa tgattttata tggtttcgtc catgccggtg gctggagcgt atttttatcg
     5041 aataagttat ccaggatgat atcaaactca gcatgatcgt ataaatgatt caactcttct
     5101 ttggaatttc ctacgccttc attgtttcct atcgtcgctg catcataacc aacttcattc
     5161 atcagcaaga tattttcctg tccattcgtt gcttctgaca atggatgcca acgatccaca
     5221 aaatcaccta gatctactgc aatcgttgtc gttttttctt tcttttcgtt ttctcgttta
     5281 cgttgatcca aaaaccttct gatccttggc caattttcta aatgagaatg cagatcattc
     5341 gtatgtaaaa tcactatttt ttccatgtac cctctcgcct ctctttatct ttatcatacc
     5401 ataggcagct ttttcttcca ttgaaacaag ctctatttca gagtttttcc tgccttgaag
     5461 caaattgtat aagtacaata ttcgataaat cagtctggct aaaaaatcag gaacattcat
     5521 ttttacagta ctataaaaat aaaaaaaaca gacccgaaag tctgttgtcg tttcattata
     5581 atcagacaca tggcggaacc tgcttagtgc tgcttccttc cagacctgac acgcttcaca
     5641 agcccaccat tgtcctagcc tttcggcaaa gaatagtata ccttattttt cattcgttga
     5701 ccagtctttt tttaatttct tttttcgctg gcggagcttt cggaaaaagt cagtcaaaat
     5761 catcccacat tcgtcttcta aaatgccacc ttctacatat gcttggtggt taaaccgctc
     5821 gtcctccaaa agattcatca aggtaccggc tgttccgcct tttggatcat acgcgccgaa
     5881 ataaacttct gctacacgag acaaaatcat cgcgccgctg cacataggac aaggctctaa
     5941 ggtaacaaaa agtgcagcat cttccaagcg ccagcttccc actttttcac aggcttcctt
     6001 gattgcaagc atttctgcat gtgtagtagc atcttgcgtg gtttctctaa ggttatatcc
     6061 tcttccaatc acttttccat ccaatacaac cactgctcca atcggaactt catgaagcat
     6121 ttctgctttc tttgcttctg ccaatgcaag gcgcatccat ttttcttttt ctactgcatc
     6181 aagatttgta tttaccaatt acccttccct gcctttgttt tttcttgttt tactttagca
     6241 tatgataaaa aggctgtaac taactttttt ttctatggta aaataaatac aagaggtgaa
     6301 caagatgaaa atggataaat tacttgctct ttacccacaa gcacagaaaa aaaactctcc
     6361 agcatcagaa gcggatatcc tttcttttgc aattgatgat gcttttatct ggatcaaaca
     6421 agattcatta tctcaacaag aaaccagcct tttgaaagcg ttattccctg taatcaacga
     6481 tcaaaaaaaa cacccatggt atcactatct ttttaaagat gcttcttgtg ttcaagaaaa
     6541 aagctttcgt ttgatccagc ttcacgtcga atcaaaagga gaattcttaa aaaaagaatg
     6601 gcaagatact attttggaat tatttcgcta ttctgctgat ttcttttttt atacagacac
     6661 agatgcgctc ttagtcgaac aaataaaccg tgattatctt gatgctactg aaatcaacgg
     6721 tatttttcta tctttagatg ctgattttga tacgacaacg tctgtattcg ttggttcttt
     6781 ccattcacct ggcaccgacc tcgtcccttt gtttgctgaa gaaagaaaaa tttttttaac
     6841 agagcaaaac aatctttata ctcacagccg tactttttct atgcaagatg tggctttaca
     6901 ttactataca aaagatactg taaaagatag taagttgatc aaaagttaca tagaactgat
     6961 caaacaaaat gaaatggaag tgatcattca agaattatgg aatgaattag gcaatgtcag
     7021 ctctgctgca aaaacactgt ttcttcatcg taatactcta aaatatcgaa tcgaaaaatt
     7081 tcaagaacag tctggtttca atctaaaaaa agccaacgat ttactgtttt gctatctttt
     7141 attgcttcat gaacataatt aaagatagat taaaggggtt gtgacaataa ttttgtcaca
     7201 accccttatt tcgaatgaat agtgttcaaa agctagtttc tcttacatca ttttttcaac
     7261 aaaatgcatt agccggtctt ttacatcttc ggttgaatct ttgattgaat caccataaac
     7321 agagattttc ttttttcctg atttgatttt tgctaacatt cccatcaaag aatccaagtc
     7381 actatctgaa agaccatcta cgatttctag taatttttca tttccatcaa atttgtcata
     7441 gacgaatttt tttactttcg tccgagtagt catatgatgg atcttttcca ttacttttcc
     7501 cgatacaacg actgctgtcg ctacactggc agcggctgct acgcttaatc cgattgttac
     7561 ttttgttgat gttttcataa gtatcagctc ctttttatct atctccatca tagcatagac
     7621 aaaaaaataa acgaactaat ccactcagtg aaatctcaat cctttaggga aaaagttctt
     7681 tactgttcct tttacttgtt tcccaaatcc gacaggtatc ttatctaata ctagcaaaac
     7741 ccatccttga ttcccatcac gttgaaatgt ttctcctgct gtgtaagatt gccattcctt
     7801 ttgagtgatc ggcaagcaag gtatattctc tatctttttt gtagcaagcg ctaatgcata
     7861 gctaggttca aatcgattct ttttgaaatc tcctaaatgc aaacctgtac gtacgacttt
     7921 taaaccatcg agagaagggg ctaactcagg aacttcccaa agatggtcgt tgaacactaa
     7981 aagtcttcca gtagcttcat aatgaaaatc atttgaaaac tccgtccaga gtttttcctg
     8041 ctcttttgtc atttgcactt tacttttttt tcgtgtcttc ttttctttat gcatctgatt
     8101 ttgtccatga aaagtcagct ttgcaacaaa atgtccttca ccctgatctt tatgaggcca
     8161 tatacgtatt gttttttcca aaccagctac gcttccccat tcagaacgtc ctgaactaac
     8221 ggattgagtc aaaggaatct cctcaatcgt tactggataa ttttctacaa gccagctaat
     8281 gatctcttca ttttcttctg gtgcaaacgt acaagtcgaa taaatcagtt gtcctttatt
     8341 ttttagcatt tttattgctg aagacaaaat ctcttgctga cgtttttgac agtatagcgg
     8401 agattcttct gtccactctt ttattgcatt tggatctttt cggaacattc cttctcctga
     8461 acaaggagca tcaaccacga tccgatcaaa aaagcccgaa aaatgaggga caagttctgc
     8521 gggtgcatga tttgtcacta ttgcgttcga aactccccat cgttctatat tttctgataa
     8581 aatcttcgca cgtttcggaa agatctcatt cgtcactaat aaccctttcc ccttcatttg
     8641 agcagctaat tgggttgatt taccaccggg agccgcacac aaatccaaaa ctttttcacc
     8701 gggttttgct gcagcagctg ttccaacgat cattgcactt ggttcttggc tatattcata
     8761 cccagcttga tggaggaaag actttccatt gacagtgcca agaaatccct cattgctgta
     8821 gggagcgggt tgtaattctt cgctatggta agtttggacc atgtccaatc ctgccggttt
     8881 tagtggattc caccgaaatc cttttttcac gcttccttgt tcaagcgcag aaaaaaaatc
     8941 ggatgcttct tcgcctaaaa gaagacgata ttttttgata aattgctgtg gtaacgttgt
     9001 cgcttcctcc ttcattccaa tgtttcgtta gacattttac aatctttttt acgaaaaata
     9061 aaggtggctg atctactttt tcatgttgct gcctaaggtg actgttcctt taagatattt
     9121 tccaagtatt catccgtcac ctcgttttta tcccaaacag cgttcatcat ttcaagaaaa
     9181 tcttgagaag aatcatctga gcgcaaactt cccaatgcat taagccatac caattcaagc
     9241 gacttttctt ctttgatctt cgtgtgacct ttgacatatg agaaaaatgg ccgcttaaat
     9301 agcgaatcca tgtctaaatc agattttgca atgatttcac gaatactttc atggatagaa
     9361 aaaaaatctt tcaataagta gacaatgctt aaaacatcat cggaactgca ttcagctaag
     9421 tagttttcaa aataaacagt gatttcttct aatatcttct cttttaaccc gctgattgat
     9481 ttgaaatgtg tataaatcgg gtatgtagaa caaccgcaga actctgctaa ctctcttgca
     9541 gtaattgact tcattccata ttcttccacg tataccatac ttttgaacag caaatattct
     9601 ttactataat ttgttactct ccccatcatt cttacctccg tctcttttca tctacccata
     9661 aataaataac ataaataatt ctaaaaatat tattgataat tatcaaaaca tatttagtta
     9721 tatccattaa aaatcaattt ttccgcctta aaaacaagaa tatcttgaca aaatacccta
     9781 ggaaataaac aatagaataa acaaggtaac aaaaaaatta aataacgtta tataaaaaag
     9841 aaggcgacac tctgtgagaa taacacagac tgaaatgact ttccactcac agtttttgtc
     9901 taactatata caaaaacaga ataaaaagga ggtatttatg ctaattgaag caagaaaaaa
     9961 agagttactt gttctacaaa tggtcattca aaaaaataat tgttctttac aagaattatc
    10021 caatgactta catattccaa aacggacaat caaagaactg atccgaaaaa taaatgtatc
    10081 actcaagcaa tttttattga ttgatgaatt tatttattct aatcataaag gagagatcca
    10141 aaccaaagaa accgatcaaa ctaaaaagtt agctgttttt tctaagttaa aattatgcta
    10201 cctaaaagaa tccaatcgtt ttaactattt gatgttacta atcaattatc cacaaactgc
    10261 tgtaccaaaa aaatatttac ttgaacaact ttatatatcg ccctcctatc tcgaaaaact
    10321 gacaaaacag ctcaatcatt ccttaataaa attccattta cggatcctat ctgtaaaaaa
    10381 ttgctgtact cttgaaggaa atgagttatc tatccgtctc tatctttact tttttctttc
    10441 agatacattt ggaggtaacg attggccatt ttcctcactt gaattaaatg cactaaaaat
    10501 gaacagtaca gatgagcaaa tagaaaaaat ttcaaaacaa gaaaaaaata tctacttgct
    10561 gttaacttta ttcatattac gaaacaaaca acaggcatat tttccaaaaa cttcttctga
    10621 aatcactgaa ttgatggact tattgcagca tacacatgat tattcagaat actttacttc
    10681 tttttcagtg agtcgccctt gtgaagagac agaaacagag agagcctact ttaattattt
    10741 tttctattgt ttttttccaa atcaaatctc ttttcgacaa aaacaggaga ttggtttttt
    10801 attttccaca agcaaaaacc ttatttgtcg acaagtcggt caattgatga cttacttaac
    10861 tcaacagttt cctctcaacc atttagaaaa taaaatacca atttattttt attttcttgt
    10921 gttggcagtt gtttttttcg aactgttggg tgaatccagc agtagttttt ctacactttg
    10981 ctttccagaa gccacgtcca atcattctag ttcttttata caggaaaaga aattaagaga
    11041 aaaaatcgat acaatttttt caaaaaatgg ggaaagaaac caaatcagtc aataccttag
    11101 tcaattgatc caacacttac tacaaacaga aacgaaagag cagttactca tccatttgca
    11161 gataaccaaa cagtttacag gaaattatca tcttcaaagc catttgcagc agttatttcg
    11221 caaagatgct gttaaaatta ccgcatattc agaacatgct gatttgatca ttacagatca
    11281 gcatgagaaa atttcagatg aaacggagac tttctatctg gatacaacgc acaatacagt
    11341 gttatttggc aagttaataa aagcggttca tgaactgtat atgaaaaaag gttatgagaa
    11401 caaaacaaag aggatgtgac aaaattcttg tcacatcctc ttattccaaa taaatggtat
    11461 tcaatcagct aactctcggt attagctcag atcaaagcgc tgatttcaca acttctttat
    11521 tttaatctct tcggttgccg ccgaacaata agatcaagcg caacagctgc aggaatgtcg
    11581 aaagcgctgc tgcaacataa gtcagtgctg ctgcaaataa tacgtgtctt gccatcggta
    11641 cttcttcttc cgtcagtagt ccgccttcac tcaaaatttg gattgctcgc cgagacgcgt
    11701 taaattcgac tggtaatgtc accaattgga aaagtaatgc taaagaaaaa gcgaaaatcc
    11761 cgatattgat taacgtagag ttccagctaa gcaatacacc taccataatc aaggggaacg
    11821 aaatcatcga accaaagtta gctgctggaa caagtgctgc acgaatcttc aatgggacat
    11881 aacccgaagc atcttgtacc gcatgtccgc attcatgagc tgctacacct atcgcagcaa
    11941 ctgaagtaga ctgtgcagtt gcttgagaca aactcaacat cttattacct gaattgtagt
    12001 tatctgtgag atctccggca atttgttgaa caccgacatt atttatctgt tgactttgta
    12061 atatatattg tgctgcttgt gtaccagtca cattgttttt gcttctcact tgatcgtatt
    12121 ttcggaaagt actattgacg tacgctgacg caattccgga aataactaat ccaagaatga
    12181 ccaaaagaaa tgtcggatct aaaaaaccat acattccata accatacatt ttcgtttcac
    12241 tccttttcgt ttactttatt ttatcattag tagtaggaaa agaatgtgaa aagaccttga
    12301 atattcttta attctttttc acaaattccg atttcagttt catcggtcca aatccttcta
    12361 ccttgcaatc gatattatga tcgccttcta ctaaacggat gttcttgact tttgttcctt
    12421 gtttaatggc acctgacgca ccttttactt tcaagtcttt aatcactgtc acgctatcgc
    12481 cctctaacag acggtttcca tttgcatcta ataccactaa tccgtcttct atgacagttt
    12541 cttgttcttc tttgttccat tcgttcccgc attctgggca aatcaacaag cctcgatctt
    12601 catatgtgta ctctgagcca cattctgggc aatttggtaa tgtcatcttc tttctccttt
    12661 aaaatcttta gtgagcacta ttgtaacgga aaaagaagat cttgaaaaga tacatttgtg
    12721 atttttacta caaaaggcac gttttcgaac gaagtatcat tctgtttttc tgctcttata
    12781 cgattggcat atatcttgga tctctttata gatttcctct acttctgcta aatcttttgc
    12841 attcgcacca cttgctaatg gatggcctcc tccatgatgg cgtttagcca attcattgat
    12901 gatcggcccc ttagaacgta aacgtacgcg gtaataccct gaaggctgct gaacaaaaat
    12961 cccccaagcc agcacttcgt caatcactcc aggaagtgaa acgatcgaag ctgtctctga
    13021 atcatcgatt ccatatttat caagaatttc ttgcggaagg attattcttc ctgcaccatt
    13081 ttcatccacc tggatatttt gataaagata accggaaagt ttagcgactt tcatcggcat
    13141 ttgttcaatc tctctgttca aagcacttgc atcaaatgga taatttctca atatcgaagc
    13201 aatctctaat gtacgggacg tcgtggaggg atacaaaaag cgccctgtat ctccgacgat
    13261 tcctgcataa agtaaccgtg cagccttaac gttcatcttt aaagattcag gaaacatcaa
    13321 agcaaattct gcaatcatct cactacagct acttgctttt gtatttaccc acacaagatc
    13381 tccataaggg tcatcattgg gatgatggtc gattttgatc agtttttctg ccaactgata
    13441 tctttgatca ctgattctgg gtgaattagc tgtatcagtc acgatgacta gcgctccttg
    13501 gtagaagaca tcttcaactt cttgcatttc tgctaaaaag cgaagccctt caatcgtttc
    13561 acctacttga cgtatttctt tttctgggaa actttcccgt aatagttcgg ctaatcctac
    13621 ttgtgatcct aatgcatcag gatcagggcg caaatgcctg tggataatga ttttatcgta
    13681 tttttttatt gtatctacta tttcttgctg tactgtcatt tcactcctgc tttctaagaa
    13741 cgttccatta cttgacaaac aacgattgct ttggaaacga tggtgttttc aataaacact
    13801 tcgatatcca gtttagcgga acgtctgccg atttccaata cacgtggacg aatgtctaat
    13861 tcgctttcca attggatcag ccgcaaataa tgtaggttca cttgttcaat caagatattt
    13921 cgtttttggt tcatcaccat tgttttttgt gcgacatcag aaatgatttc gctaagtacg
    13981 ccaaaagaaa tggttccaac gccattgacc atttgtggtg ctactacgaa tttgaactta
    14041 gggttttcca aacgatttcc ttcacggtca gtatcaatcg tatggatatt tccagatatc
    14101 tgatccgaaa tcgtatctgc aatctgcggt tgtcgctgga caagctgcat cgctttcatt
    14161 acatcttgcc gggtaatcaa gccttccaat gtcaagtcat cggaaacgac cggcatgact
    14221 tctagcccat cccagatcat ttgatggcta acagaggcaa tactcattgt tttctttaca
    14281 ctgattggtt ctttcgtcat cacccgatcg atgatctggt ttggcgattt ccctaaaaca
    14341 tcttttgccg taacaatccc catcaaacga tgatggcggt tgacgactgg aaaacgcgaa
    14401 tgctgcgttt cttctgacaa acgctgataa tcagaaatcg tatctgtctg ctgcaaataa
    14461 tgagtctttt cagctggcat atagatatca ctgacaagca tgatatcttt tttgatcaat
    14521 tggtcgctta acgctcggtt gatcatggtt gcgactgtaa atgtatcata agaggtgcgt
    14581 aagatcggca tttcaagatc atcagctagc tttgcaatct cagcagtcgt attgaatcct
    14641 cctgtgatca atactgcggc gccatcttcc aatgctaatt tttggacttc cgcccggttg
    14701 ccgacgatca ttagtgatcc tggcgtaata tagcgttcca tggcatgttt agtcatcgcg
    14761 ccgatgacga atttattcaa gaccttgtct aatcctgccg ccccgcctaa aacgtcccct
    14821 tcaatgatcc tgacgacctc gccaaatgtc aaacgttcaa tatgtttttt taatttgcgt
    14881 tcgatacgga tggtccctac tcgttgaatt gttgaaacta gtccgacatt ttctgcatct
    14941 ttgattgcac ggtacgctgt tccttcactg acattcaatt cttttgcgat gcttcttaca
    15001 gaaattcgat tgccaacagg taaactttca atatattcta aaatcaaatc atgttttgtc
    15061 gccattttct gcctacactt ccagcgtttc cgagacgctc attatttttc cttcactatc
    15121 ttccagtaac tctacaaaag attcgggatc ttgttcaatg acagggaaag tattgtaatg
    15181 gattggaact actttcttgg cacgaattgt ttgcgcagcc tttgctgcat cttctggccc
    15241 catcgtatag ttatcgccaa tcggcaagaa agcgacatca atatcgaatt cttctgcaag
    15301 caatcctaga tcactgaaat aagcagtatc ccctgcatga taaatcgttt ttccttctgc
    15361 ttgaataata attccgctag gctcgcccat atactgaacg ataccgtcta cttcatagct
    15421 tgaactatgc tgtgcatgta cgaattttac cacgccgaat ggaaacgcat gttttccacc
    15481 gatattcata ccgaatgaat ctactcctct gctgttcgca taatcagcaa tctcaacaat
    15541 actgataatt gtggcttgat tttcttttgc aataggaatc atatctccta tgtggtcact
    15601 atgaccatga gtaatcagca accaatctgt ctttacttct tctgcagtta agtctgtcaa
    15661 tggatttcct gtaataaacg ggtcaatcat cacgcgttgg ccattttcca tttcaattgt
    15721 taaacaggaa tgtccatgat acgtaatttt catattttac acctcgcgtt ttctctgtat
    15781 cttttttatt aaaaacgatt tcatcacttg cacatcaatg aaaaaatata gctgctaaat
    15841 atattttcat tggattgata gtacagtatg tacaaagcaa ttgtaacaga taatacagga
    15901 tgcaccaagt accaagcaga tataatctca agtttgaggt ctctgctctg cttagcttct
    15961 cttctcgtcc cttaactaat taaatcagcg taaataggat ggacgatctt ttctaaatcg
    16021 gctactttta tccgaaggct tctcccgatt tcaccgcttg acacaattac ttcttctttt
    16081 tcttttaaag aactgtctgc taaaaaacaa taatcaggat gtgcgagttt tattccaata
    16141 ggggtgtttg ctccgtgggg atatcctgta tgcattaata caaaatccat tccaggaaaa
    16201 ccaactttac gatctttcgt cacttgtctc gtttttttgt aatctaagtg atcgttcaaa
    16261 ggaatcaatg cgatgatcac cccagttttg tttcctttca ctacgagagt tttacatatc
    16321 aaagctggat caatccctct tttttgtagt tcttctgtga aagattcttc taccacatga
    16381 ctaaaatcaa gtggttgata gctgatcttt tgttcttcta aataacgttc gacttttgtt
    16441 tgactcatca ttgattcact ccctttattc tatcgtttat tataaagcga gggatgctgt
    16501 attcaaaaat tatttgtttt tcgcaaaaaa agagcaagac gagcactcta tagagaggtt
    16561 catcttactc tttttgttta ttttttattt cgacgttgag tagcttccat aatgactggc
    16621 aagatcactg ggcggcgttt tgtttgttca aataagtaac ggcctaattg atctcggatg
    16681 tcttgcttta atttgctcca ctcaaaatca ttgctttcta aatgtttctc aacgattgtt
    16741 tcaatcatat tgctgctttc tttcatcaga tcacggctag cttttacgta aacgaatccc
    16801 cgtgaagtaa tttgaggttt tgaaatgatt ttcttttctc gccgactgat agtgacaacc
    16861 gctacgaaaa tcccgtcttc tgacaagatt tttcgatcac gtaaaacaat gtttccgata
    16921 tcacctatgc ctaatccatc aatcattaca ttttctgcac ttgtagttcc tgcaacactc
    16981 atacgcttgt tcttatattc cagcacgtct ccgcgtccag taatatagat atttttgtat
    17041 ggcatcccta ctgcatgagc caaatctgca tgagcagcta attcacgata ctctccttga
    17101 actggcacaa aatatgttgg tttcatcaaa ttgatcatta attgcaagtc attaggattg
    17161 gcatggccag aaacgcgcat attctcagag atcaacttca ctgttccacc agctcgataa
    17221 atgatatctt ccgttttcgc cacgatggtt tccatcgcga tgcttggcgt agtagtgata
    17281 taaaccaaat ctccttcttc aatcttgaca ttacggtgtg taccgtttgc cattttttgc
    17341 agagacttga tgggttcacc cattcggcca gtttctaaaa tgatcagttc ttctggatca
    17401 tagtttttca tgtctttcaa cgtaacaagc agatcttcac ttggtaattg aagtttttct
    17461 aaacgcatcg ctgtgcgaat aattcgttcg aaatcttgac cagtcaagac aacttttcgt
    17521 tgtgcttttt ctgctgcatc caatacttgt tgcactcgtt gcaaattact tgctacacaa
    17581 gcgacgatga tacgcccttc ccaatatttg atcgtatcaa atacttcatc tgctatttgg
    17641 gcttcagaag ctacaggctg aggattttct gcatttgccg attcacttag taaggctaag
    17701 actccttctt tgccgatttc cgccaatcgt gcataatctg tttgatacat tgggatagcc
    17761 gtttggtcaa atttaaaatc tcccgtataa acgatactac cttcttttgt tttcagattg
    17821 atccccactg aatctggtat cgtatgagtg gtacggaaaa agctcactac tgtttcacca
    17881 aagtctattt ctgtatgctc atctaccaca tggaaatcct taaatccttt ggctgcttca
    17941 ttacgactga cattcaattt tgctaactcg atcgtcaact ctgtcccgaa tactggtaca
    18001 gaaatttttg acaacaagta aggaagtgcc ccgatcgcat ctgcatgtcc atgtgttaaa
    18061 aagacaccag ctacacgatc aatgttttct tctaaatacg taaaatcagg gattacgacg
    18121 tcgatcccca acaactcatt ttcaggatat tttagcccac aatctaaaat aaaaatatcc
    18181 tcgcccactt ctgcgacata catgtttttt ccattttcac gaacgccgcc taatgggata
    18241 attttaatgg tactcacaaa cttcacctct tcttatgaca tccaagttta aagatgtcga
    18301 ttttcttctt tatctaaata tatgagccat tccaatgaag catatgcaaa atcactattt
    18361 ctcaacttat ggagaatcag caggaaaatt accagcacat ccgcgttctg acagttggat
    18421 gggcatttta ttttggatat acttcattaa caaaatggat cttttgcgaa acgctatagc
    18481 cagaatactt atataagtat tcagctgttc cccattatta tacaacaaat aaactaaatc
    18541 gctagtatca gcaattgtta gaacaactat gcacgtaaaa atccctctgt agcatctagg
    18601 atttcttttg ctattcctct ttcatttctt cttcgtcggt ttgaaacata cttttttcat
    18661 tttaccacat tttttgcaat aactatagtg actttttata tatacagaac ctcatgttga
    18721 tggtgtgcaa acagcataag atttaagcta gctttaagat aggttgtcta ttatcaaatg
    18781 catctactag gagggatgca aaatatgaat tttatgaaac gtgcatggaa aagtacaaaa
    18841 gtaaaatggg gacgttcatt gttattgttt gccgtgttca cagccattct tatttttgtg
    18901 ctagctggcc tcacgattcg aagcgctgca gaagaagcag cgaatcaagc acaaaaagaa
    18961 gttggagcaa cagtgacttt aagtgccaat cgagaagctt catttaaaaa acaggaacaa
    19021 acttcttcaa gcggttctac cactgaagaa cgtccagatc caggcagttt ttctcttaca
    19081 ccagtttcct taacggacgc ggaaaaaata gctgaattag ataatgtaaa gagttattcc
    19141 tttgaaagtt ccgcctcagc tatggcaaaa gatggaataa ctgccatcac tagttctgat
    19201 tcaagtgaag acagttcaga aactactgat gcaggcggtg aacctgctgg aggaatgggc
    19261 gggaatccgc aaatgactca agcagacttt caaatcagtg gtgtgtcagc aactgcacaa
    19321 aatagtaatt tttctgatgg aacagcaaaa atcacggatg gtgaaggaat agatgacagc
    19381 gacaaaggca caaacaatgt agtcattgat tctactctag cagaaacaaa tgacctggca
    19441 gtcggagata ctttcacaat cacgagtact gaagatgaag ataccactta tgaaatgacg
    19501 atcaaaggaa tttatgaatc cagcgaaaca agcggcagta tgggtatgaa attcaacttc
    19561 atgaatccgt cgaatacact ttacacttct tacacttttg caaatgaact aaatgggaca
    19621 tctgacgata acacgataga ttctgctatt tacactctat ctgatccaga taaaatggat
    19681 gagtttctca aagaagctga gaagctgatc gacacagata catttagctt acaatcaaat
    19741 gattccatgt atcagtcaat gttggaacct ttaaacaacg tggcaagttt ttctaaaaat
    19801 gttgttttac tagtggctgt agccggtatt atcatactta ctctgatcat tatgattacc
    19861 attcgggagc gtcggcatga gttaggcgtc ttgctatctt taggagaatc cagaagtaaa
    19921 atcattctcc aattgtttac cgaagtggct atttgtatga ttcttgcatt aggagttgct
    19981 tcgcttagcg gaaacgtcgt cgcgaatgcc gtgggacagc agctacttga ccaacagaca
    20041 gaaaccacga cacaaaacca gcaagctcct ggcggtggac aaatgccagg taacagacaa
    20101 gaacaaggcg gtcaacgcga tggtcaacaa ggaaccacga ataatccatt tgctgtttct
    20161 gatcaagtca gcgaattgga aatcaatgtc aaacctgcac aattgggact attagccgga
    20221 gtagcatttg gaatcagtct gttttctgtc tttttagctt ctatagggat attaagatta
    20281 aacccaagaa aaatcctatt aaactaaagg aggaatttga tgttacggac agaacaattg
    20341 ggattttggt atcaagatcc gcaagatgcg ctttataaag atgtagaact gaatttcgaa
    20401 gctggaaaaa tgtacgctat tttaggagca agtggttctg gaaaaacgac ttttctttcg
    20461 cttatttctg gactagatgc accaaaagaa ggaaaggtat actatcaaga tcgttcactg
    20521 aacaaaatcg ggttaagaag ttatcgtaaa aatgatgtct cgattatttt ccaagcttac
    20581 aacttgcttc cttatttatc ggctctagac aacgtcatag cagcaatgga aatcactaag
    20641 accaagcaag agaattatcg acaagttgct ttagatcgac tggctcgtgt aggaattgat
    20701 gaaatattag caaaaaaaca ggtgaccaaa ctttcgggag gtcagcagca gcgagtcgct
    20761 atcgttcgtg ctatctgctg tgaacatcaa ctaatcgtag cggatgagcc tactggaaat
    20821 ctagatgaaa caacttcaca agatatcgtc aaacttttcc aagaaatcgc ccatgaacaa
    20881 aatcgctgta ttatcatcgt gactcatgag attgaagtag caaaaagctg tgatgaagtc
    20941 tttgaactaa aaaataaaaa atttcaaaaa atcgaagttt aaaaaacagg ataatgatca
    21001 aataaaaaat ccgaaccata tctgtctcgc agatatggtt cggatttttt atttgcttga
    21061 ttcaattagt aataagtggt ttttattttt tttcgtcttt tcgagaaaac ttgtctttga
    21121 cttcgtcgcc taaatttcga gctttttctt tgatgtcagc acctagtgat tgtgctttcc
    21181 cttctgtttc tttgctcttg tcgtctgttg ctttgccgta tgcttctttt ccttcaccga
    21241 ctactttatc tttcatatct ttagaacgtc cttttaaatc tgccattgtg cttcctcctc
    21301 ttagtttgtt tattgatcta ccttcagggt aacatcaaca acagatacag acaaacagaa
    21361 cgctcgtttt ctaaaaaaga tagttagagc tgttgattaa aaactttttt cgaaagtccc
    21421 ccatactttg acttcttttt cttggaccat tatttctcct ttagcgataa ctgtatcaat
    21481 atcaagcgtt tctttatcca agatacacag atctgcatca gcaccaattt cgatttttcc
    21541 ttttttatcc aatttcaata tacgtgctgg attactggtt atagctcgta aggcgatttc
    21601 caaagggata ctttcttttt gcacagcttc tttgatccct actagcaatg ccttggcact
    21661 tcctactcct aaccctagga aatgattgtt ttcatcaaaa taaggcaagc ttccttgtcc
    21721 atctgaggtc atcgtgaaat tatctaaaga aacatcttcc tcgatcaatc gtttcagtgc
    21781 tttgctgaag cgaacttcac catctgtctt ctcccaaaaa tcaggatctt cgctagcagt
    21841 gaagtcaatc gtgccaccac gtttggcaaa tgccacgcaa gcttcaaata attctggtgt
    21901 gcgatttgca tgagtaggca aaaattgtgt gatcggaatt tctgtttctt ctacgattcg
    21961 tgtcaatagc tctagctttc tttttcctcc gcctaagtgg acattgatga tcccagcttt
    22021 tccggataac atgcctccta cacgagcatc tgctactgca cgagcgaact cttcaaaact
    22081 tggctgagaa gaacggtgat cggatactgc gatttctccg attccgataa ttttatcaat
    22141 tgtcaaaaag tctttgatga tagaatctgt tactgttttt accggcaatc ggtacgaacc
    22201 ttcgtataca taagtggaga ttccttctgc ttccagacct ttcgctttgc tgatcaacgc
    22261 agtcatatct ctaccttctc cgtctgttcc caaacaaccg acaactgtcg ttacaccagc
    22321 tgtagtcaat tggctcaatg tgacttctgg cgttcgattt tgataaccgc cttctcctcc
    22381 tccacctaaa atatggaaat gacagtcaat gaaacccggg gttagtactt ttccctcacc
    22441 tgaaagttct tctacttcca ctccttcgta gccgccttct agctgttctt ctacagccaa
    22501 aatcttccca ccggcaatca gtacatccat ttttcctaag taatctggtg caaacacttc
    22561 aatttgtcga atgattttca tacatttttc ctctttcttt aagtataact gatgatagga
    22621 tcttaaaaaa taatccaaac gataggatct ttactccttt gattttaccg taaaatgacg
    22681 gttcttggag tccttatcta ctgtttggat tactttttta tttacaggtc tccagtacta
    22741 ctgcagacct attgcaatgg caatcatgat gaaaatagtt cctaaaacaa acaggaatcc
    22801 ttgcatcttg atttggaatc ttgcccattt tgcccattcg atccgtgcaa cacctagcac
    22861 accaatcaag ctggctgata cgggtgtaaa agcgtccata aaacctgaac caagctgata
    22921 agcaagaact gctacttgtc tggatacacc tactaaatct gccagtggag ccatgattgg
    22981 catagtcaaa gctgcttgtc ctgaatttga tgtaacaacc aagttgaaca aactttggaa
    23041 gatgtacatc gcccaagcac caatgacagc tggaacaccg gtcaacgccg tcccgatccc
    23101 atgcaaaatc gtattcaaag ctgaaggaac agatgcatca gatcctccca agacaagaag
    23161 aatccctttg gccataccta ctacgattgc tgtaccagct aaatcagctg caccagattg
    23221 gaaagaagaa gcgatgtcat tgactttcat tccatctaat ttgaaaagaa cagcaatgat
    23281 tccagcaact aatcccatca caaagaattg agaagcgatt tctggaatgt aatagccttt
    23341 ttgagtcacg ccccaaacga tccagacaag gacaactaac atctctaata agatcaattt
    23401 atgcccaagc atgaattttt tattttcatc agatgtcttt ttgatatgag aacggaaata
    23461 atcatctgat ttgtaagtca acgaactttc tggtttttta cggatttttt ctgcatatat
    23521 catcatataa ccagcggcta atgcagtaac aactacccac ataatcaatc ggaatgtcgc
    23581 accagacaac acggggattc cagcaatccc ttgagccacc gctacgctaa acgggctcat
    23641 ccaagaggtc gcatttccga cttgagatgc gacataagtc acggttactg ctacgatcga
    23701 gtcatatcct aacgcaatga caaatggaac catcaccatt gagaatggaa ttacctcttc
    23761 tgccatcccg aatgtagcgc caccgaatga aaaagcaaag aataatagcg gtaaagctaa
    23821 acgctccagt cccttagttt tactgataaa cgcatagata cctgcgtcga tcgctccggt
    23881 acgcataata ataccaaaag cacctccaac aacaaggatc aatgcagcga tccctacagc
    23941 agatccatac ttatctccag atactagtcc ttcaaaaaca aaatttaaga aaccaaagcc
    24001 gccaaaatca tctgttcccc atattttggc tgttttatgc aattttttag aagtatcata
    24061 gatattttcg ccgtactgat cgtaaagcac atcatctgtc aaggagattt catctagttt
    24121 ttcctgcgta agattttttt ctccgtcagc aatgactttt tccaatcctt tttccggaac
    24181 atccagtttt tcacgttctg ctggatgatc ttgcagttct tctaattgat caaacacata
    24241 cgacttatcc agctcgtaag cataacggaa ggaatcttga cgtaaaacag tacgagtact
    24301 tgtttcgcca tttgcatctt tgtattcaat atcttgcgtg ctgaactttc ctgctggaac
    24361 gacaaaagtc agtatccatg caaagatgac tacaccaaaa ataatgacat acgtatgggg
    24421 tgtcgataat ttagtgaagt cgcgtttcgg ttttcctgaa gaatttgttt tcttcaaatt
    24481 tatttcttcc tttctttttt cagtaatttt gatgaataaa aaatggataa tcaaatgaat
    24541 tatacagtaa aaatgacatt tccacatgaa taatttatta gaaaatcact tttttctatt
    24601 ttgttttttt tctcctatgt atgattattt ttttctcttt ccatttatat taagtatttt
    24661 atttctctgt ttttccttaa aattcttttt ttatttttag attagagaaa atctcgtcct
    24721 gtttttttca ttttacaatc attgaaaaat tgaatttcat tgcaaaaaaa ataataaaca
    24781 cttataaata tccattatct atccttcaat ttttgtgtat tttatttcta ttagaaaaag
    24841 gtatcctgtt ggacaatttt gttatatcag gtaaacttca ttgcaataat gataacaaat
    24901 gtactataat tactttgtaa agagttttgg ggaagactct ttcatcaacg tgtattaaga
    24961 gacaagaaag aatccatatg gattctttct tgtctctttt tccactactt tttatttttc
    25021 tgacactaca tattcttata actcatttct cataactgtg aaacaactta taaaaataac
    25081 aaactttaaa caaaacaagc ttaaaatcaa agatgatcca tcttaaaata actaaaacaa
    25141 aaaaagtagt cgttgttaat cctcattaat aatcgttttc tttttttcaa acgctccatt
    25201 tttatgtgga acattatttt tttaaggaac tttgtcaaca tgtggataac tattttccac
    25261 ttgtgtcctg gactagctgt tttgctgtaa aatacaaaac ttgctgcagc aaaaagatcg
    25321 ttgcaagaga ggctgttccg cagatcgctg gatctagatt tggcaaagtc aagactgcat
    25381 catgtaaatt ggcgtggttg tccaaaacca aatcagcaaa ttcaaataat cgcaaaccac
    25441 tgctatgttg cgatttgata cttcgtgaca aatcaaatcc agtaacaatg attaatggat
    25501 gaccattttc tttgatccac tgagctagtt cgatgattgc cgggtttctt ccttcattcg
    25561 aaagaagaag aaaaatgtct tctgatttga cagaaagctg atcaataatt gcctgtcctg
    25621 caccttctag cgtttcatag acacctccgg ctggattttc aatcaatata cttggtacaa
    25681 gatccggggt ttgatcccaa aattcaaatg ctacaccact tagtgtgcgg ctagcatata
    25741 tctgcaatct accaccttga ttaatttgtt tcgcgatcca agtgccagct tgaagcatca
    25801 gcggctcttc tatccttaat ttctcttcca attggtgata atagtcaaaa taacataaac
    25861 tcatttatct ctcccgccta gctatacttg acatgaatag aaacagctgc ctaatctaag
    25921 gaagtacata aaaaaacagt agttagacag taacacatgt ggtctttgct gtttcaaaca
    25981 caaaaaaggc attgaacgaa tacgcccaat accttgacat agcaaataaa acaacgtgca
    26041 gaccatggaa aatatctaca taggggtaga attttcaccc tagattaggt acaataaatt
    26101 aggtcgatgc actgcattga ctatggcaaa atgatcggtc agggtgtatg tgtgtgactg
    26161 gtcattccct gattcttagc gccaactaag ggtcagggag ccttttttta ttcagctgtt
    26221 tctatctgaa tttattgtaa gtaatctctt atccaagtgc aagcaagaaa atacaaaaaa
    26281 acattggtat gatttatata tatataaata acataaaaaa aggaatggct tatcccgata
    26341 caacaagcat tcctttaaaa aagattcttt tatataagtg aaaaaagctt ttttccacca
    26401 attaaataaa tctctcatct caatagttta tccattattt tatctttttt tgcgtatacc
    26461 tactaaatat taccgtcttc ttctcgttct tctactttgt tcgagagaat ctgtactaga
    26521 aaaagtagtt gtatgtctac cttttctgcc tatccgagac tttcttttca tatatgggat
    26581 ataaatgatt agatacagta cagcaaaaaa taaagcaaat acgcaagctt cccaactacc
    26641 taacgccaat tcaaccatca aggtagcaac cccaacggat gctgcatacc agataaaaat
    26701 cagaaacgtt tctttttttc tgaccatgcg aaacatgata aatagactaa taagcaatag
    26761 tggcaacaca aatatccaat tgcttccttc catcagccac ttgagtatat caagcagcga
    26821 ctgtgcaaca gcgactaatt ttcccatagt gaacacacac ctttttttat aattaaaaaa
    26881 ttcgcctttt tattctacta tgaatcttct ggcttggctt tcatttctct tatgtaatca
    26941 ttttctttta tacaacttaa aaaaaaatta aaccattagc ataatatttt ctgaatctat
    27001 tcacgaaagt ctttttctat ctttcgtttt ccacgactca actgatctag agtcaaaaaa
    27061 ctaaaacgac agttgcgcaa atgacgcaca agctgccgct ttaataacaa atagcctttc
    27121 attctcagca ggccacttat ttccagttaa ctttctgttc ttagcttttt actcaactgg
    27181 ataatatatg cacttaatga agtcccaatt tccgaatgtt gaagtccata ttcaatattt
    27241 gtcttcatat atcctaactt atcgcctacg tcgtatcttt ttcctttaaa ctctctcgca
    27301 tagaccgcct ctgtctggtt aagagtctga agtgcatccg ttagctgtat ctcgtttcct
    27361 gctcctggtt cttgagtttc taatatctca aatattttag gagtcagtag ataacgtccg
    27421 ataatggcta aattacttgg tgcttttccc atttcaggtt tttctacaaa cccttttact
    27481 ctgcaacgac ccttttcgct atctgtttta ggatcaataa ttccgtattt agatgtttca
    27541 ttttctggta cacgcatgac ggcaacagcg gaagcttgtg tttcttcata atccatcatc
    27601 aattgttgag tcaatgggat atcatctttc atcaaatcat cccccagcat gacgacaaac
    27661 ggttcatttc ctataaatgc ttttgcttga agcacggcat cccccagtcc tcttggatga
    27721 gattgacgga tgaagtgaag attgacttga gtcgtttctt caactaaagc gagtaattct
    27781 gttttttcct tttcccgcaa attgttttcc aattcgatat ttgcatcaaa gtgatcttca
    27841 atgggtcttt tttcttttcc cgttacgatc aagatgtctt cgattcccga atccaatgct
    27901 tcttccacaa taaactggat cgttggttta tccacgattg gaagcatttc ttttgccatg
    27961 gcttttgttg ccggcaaaaa acgtgttcct agcccagcgg caggaatgac tgcttttttg
    28021 accttcactt gct
//
LOCUS       DASHHB010000033        25576 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.034, whole genome shotgun sequence.
ACCESSION   DASHHB010000033 DASHHB010000000
VERSION     DASHHB010000033.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.034"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            150..278
                     /locus_tag="U2K71_001640"
                     /pseudo
     CDS             150..278
                     /locus_tag="U2K71_001640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
     gene            392..631
                     /locus_tag="U2K71_001641"
     CDS             392..631
                     /locus_tag="U2K71_001641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315723.1"
                     /translation="MARPVLEAMDMDTLTKGRYVQLLARGYSPKDVFKSLSKGTDMEK
                     ERLRKEFDYWRTHNGIKDLKPARPVIKRKKKITQD"
     gene            743..937
                     /locus_tag="U2K71_001642"
     CDS             743..937
                     /locus_tag="U2K71_001642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315724.1"
                     /translation="MEEKLLKTMKQKHLKRLSVMQYINDMQITGKEKACLLGSMKNFE
                     QLRRTYVKTSSNCQLLLEVS"
     gene            1023..1694
                     /locus_tag="U2K71_001643"
     CDS             1023..1694
                     /locus_tag="U2K71_001643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class A sortase"
                     /protein_id="HEM7315725.1"
                     /translation="MRKWLFGFLGVALIVFCSVFGYVSYQKHEGEVFKQNIEKKMPVD
                     QINAHAKSYKEDATNVNNDMSLGQMLSIQQEAIEMGVNKQVFAQIQIPALGLALPIFK
                     GANQYTLSLGAATYFYEDAEMGKGNYVLAGHNMEMPGVLFSDIQKLSLGEVMDLVSND
                     GVYRYKVTRKFIVPEYFKLIDGVPEENSFLSLPKKGEKPLLTLFTCVYTSQGKERYVV
                     QGELQ"
     gene            1747..3723
                     /locus_tag="U2K71_001644"
     CDS             1747..3723
                     /locus_tag="U2K71_001644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEM7315726.1"
                     /translation="MAINRYVKKAGMLIGMLGIIAGCFQGYRAYAEDVTQKTPPEKVN
                     ITVHKLMYDQGTQLNVDIDGIKNDGYTHDQYPTGVTKYNKADYGDVEFTLGNITDQVL
                     PREDSDLTNAKVDEIVQDVEDKGSNSEYVKNATNKITSAVDENGEITFTDQPAYVNSK
                     GNVYVVYESKSAAGLVTQKAKPMVVIAPMTDKTGSGFLKNIHLYPKNIVSKLSFELTK
                     FGDDGADQSKQTLLKGAKFELYKGEPGKGTKLGDLVSDDQGKLTATDLTLGKYYFVEV
                     PSEVVVGSDQEPTADQYLLGADARNDAHNKLTFEITNDGVTSDLKASYVNYKAPVIDK
                     TVTNGTGQEHSFQIGDAVNYQGTIHIPTDIAGGADGITVNGVKSETSPYSVFKWGDTA
                     GQGLSYVAAKANIKVTNKDGSVVLKENTDYKIQNSENGFVIDFIVNNGQVSDTVASLH
                     GQDLQMTYNMYVNDSAAVANPLTNSVDFVYNNNPFNQEEHHEKTKADVVTYGAKFLKV
                     DSGLFGTGIKATPLEGAEFAAKNAEGKYYGGLVDTDKDGVKEAVWVDDVANAAILKSD
                     KEGHFEITGLTEGEYSLEETKAPENYQKLTKEISFKVDKDSYKEENRITIKNNQKASV
                     PMTGSNGFQTYVLISCLLLGAGALSAVVYFKKKA"
     gene            3738..4490
                     /locus_tag="U2K71_001645"
     CDS             3738..4490
                     /locus_tag="U2K71_001645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM7315727.1"
                     /translation="MIIRKPKKKRIMGKWIIAFWLLSAVGVLLLMPAEASVAKYQQNQ
                     QIAAIDRTGTAAETDSSLDVAKIELGDPVGILTILSISLKLPIYDGTSDKILENGVGI
                     TEGTGDITGGNGKNPLIAGHSGLYKDNLFDDLPSVKKGEKFYIKVDGEQHAYQIDRIE
                     EVQKDELQRNFVTYLESNPNEDRVTLMTCTPKGINTHRFLVYGKRVTFTKSELKDEEN
                     KKQKLSWKWLLGSTVFLSVMIIGSLFVYKKKK"
     gene            4506..5261
                     /locus_tag="U2K71_001646"
     CDS             4506..5261
                     /locus_tag="U2K71_001646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315728.1"
                     /translation="MRNRKWLFVCAAALFSFTAYTQLTFADRQESTDSTEQTIQTSDS
                     STIQSSDETVDSSTVDSSEEPKIAEDDQIVKQTIHVQKIISSEQLDNQGHEKQEGVNG
                     AKFSVYDVSDILQKMDVKDLTTDQIESQLKNRVKKLSSDQLKLVSNEETKTIDQQTGV
                     FEFSVEVQANQKQAYYIVNESSPENISNSEDILLLTPVSDKNGEFLKDVWIYPKSEAS
                     QPKEEVKKIVSTGVKKNFFENCWDFVTHLFSRD"
     gene            5274..5534
                     /locus_tag="U2K71_001647"
     CDS             5274..5534
                     /locus_tag="U2K71_001647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315729.1"
                     /translation="MKLIKLLLFNFSCLTAVFLYCGLLFSKLSILPSYQLYLMRILNI
                     PAASMPLDIMEKLIRNPLFTTINLIVIVGISLVIYKKEGMKG"
     gene            5531..7621
                     /locus_tag="U2K71_001648"
     CDS             5531..7621
                     /locus_tag="U2K71_001648"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEM7315730.1"
                     /translation="MNFKHSWTRRLKYVFPIMMMLGTLFGLKTANVFAEKVIVDPSNP
                     IQNVKNNFIIPKDVPNAKTNITVNGASKVYKYHVDANKGGFTDDYVGLVEGNKNIRYP
                     SFQKEYGETNLVLEGVTTNTKVNIDYGKIGTYNGKKVNIKLVLSNIHLYSDTLPWNLL
                     DNNYTKTHFRDDGYKNTNGAMSKSKKRTVLWISDNLFSGIVYHSTQMNVQLVATYEDG
                     SPVQFSGDTFISFNSLNPAGGKSTDLKGEYAHYDKMNTTDWYVRKDTVLSEFKSFYNN
                     LNVVGGHPGGSSKLTQADNDFNNLHDKLGDPKFGQGTVSFKISEANPTFVIGSSNVQT
                     WFTLSSATIFSVVPDQPEKTGVDKNGNNVNDKMLQVGDTIQYRIKQKVNRLGVDLLAV
                     YDRFELIDNLPKEVNYVDAHVESGTNKKFDVSGEVTYDKTKHQVKYAAKADTLKNRMK
                     YNGETYELVINVKVNELANQNSVAKNQGTSIINKVEKDTNIITVYFPKIPVKEVQQNG
                     KDVNGRNDGKKGTPTAPLNAGSEVQYLVTQKWHTKGVDAASDHYKQFSIQDPIEARLT
                     YKEGSAQVIDKSKGKDITSEGTLTYDSNSRTLKWEASADFLSNNLLDGREIQLIFTAK
                     TPLQSEKNIDNQAVVAVDNVSNKTNVVTIGVDPNLPQVIVPKTGSTHLVTISAVSLLL
                     LVLATFSYAVLRYI"
     gene            7648..8112
                     /locus_tag="U2K71_001649"
     CDS             7648..8112
                     /locus_tag="U2K71_001649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315731.1"
                     /translation="MKKLIIVVTSLIAMIVILVGCSNEKNKQTNQDNGVLKSGTMWKE
                     EVGGLVYNLKIIDETTWEYSESVWHPDPVQITVKRQKDYKGLERYKIVDSAGVREFIN
                     KSDSLFIVVPYEKNGVKKIIFLGSSKDEKRTKEKLIHDGSQSNKYKLQKTSE"
     gene            8109..8351
                     /locus_tag="U2K71_001650"
     CDS             8109..8351
                     /locus_tag="U2K71_001650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5415 family protein"
                     /protein_id="HEM7315732.1"
                     /translation="MIASLFICPEGGITVAKAKTPFEKAASKILERRGVSETQWKQEV
                     LEKAQFDLLRGKDKDLEGYIYQREREKLVFDEIIKH"
     gene            8369..8671
                     /locus_tag="U2K71_001651"
     CDS             8369..8671
                     /locus_tag="U2K71_001651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315733.1"
                     /translation="MFESITMNFLTQPKVVAAVDIFRALQTAATSLKNNLMIFAAAAA
                     VASIVIGGLFFLFGRRGAENGKMHVGYVLMGVGVILAAASIVVSLFGLFGQTPQGL"
     gene            8741..10804
                     /locus_tag="U2K71_001652"
     CDS             8741..10804
                     /locus_tag="U2K71_001652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315734.1"
                     /translation="MYFTIAGNTKTDKELIKKATKEVGKRTSFLPKTIKYSVIETEEQ
                     LHVLGKVLEHESRSSVKLTLTLHDTEGKIEKAGTEYVFGPLDYQISEGFYPTLMDLIR
                     LDVFKETDETMSVEESYRNEKLLQVLEEELESDQRKVNQGSEVEVNEEVPWERPIFPK
                     KESKEKETVYVDEQADIPTQKSIFEEENDVMGQLEVDPFEETMEDEETAEVEVDPVEA
                     NEESTWTEGDSQEYIEPLISEEVEKNEIEIQKATVFDYILDQYPPEHEWMKEKNKLFL
                     NELYSKYQLPQTYQAFLESKEELISQGKGTLLEQLESVQSTNWHELAQEDLLATFEER
                     QQETDQEINEYATEQSSNWEKAKEELNKEEQRVIEEEVQKIKERYEKKRDNAYSLCVA
                     KINNFTQTNQEALSKEKENLLKERIKERKEEFYRGLRTDKLKISKELNEQLNKLYQTT
                     TEVLMEKYEKIQQELQVQIPKWKKEYEIQQQELKEQEEKQRQKEKEREQLELKKREQD
                     LAEQQMAIEKQRLNQEKEKEAAARKERQEQMDTLRQAQLNYLAQPQPTPSVAAVQTPM
                     NNQTNLTPSPQRSFNGWMVGCIASLSLLLGGGTVFAFNHINQSSQADSVATTTSIKEE
                     AQEQARKEYEQQLEELLNRTNQSTTESSMENSKESDSSKAKEGSSDSTVQSNTSESSE
                     SNEVK"
     gene            10804..13416
                     /locus_tag="U2K71_001653"
     CDS             10804..13416
                     /locus_tag="U2K71_001653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV secretory system conjugative DNA
                     transfer family protein"
                     /protein_id="HEM7315735.1"
                     /translation="MRVRETTKRKDKRFNLEKNRYLSHFYRYTPSGEELPEKHDKRIS
                     KKIFYSGLVYLPLEYLVTRVPEVFNRLEIVKGSLQGINLFQLQEMPQVFHFDSAGFWN
                     PFTPTTSLGTRLAISTVCVVPAVLMGLKMDMWFRPLADGQKGDNRLMTLKEIQQIYPQ
                     IPETKTTFPGYGGIPITHYKKYWYIQTDTVNTCIVGTSRSGKGQIEVLATIDNLSRAE
                     KQSSMVVNDPKTELYVGSKDELEARGYDVYAFNITDPLQSMSDNPLALIVKYWKRGDI
                     DTATQLTNTFTNTIYYDANASDNKYFNENAQKAVNALIFALLEQADQTGDYSKLTPNN
                     LVELLSEIGGFNYYPDPDNEFKQLNALDEFMNQLPPGNIAKKQYGATKIGSDKAKGNI
                     LSTAITGLNPFTLTKIAKMTSQSTIDYKSVGFPKYLDLKLHESLLNQRVVVEFYHKGK
                     KFHEERVKVGYKGFCEINFDCHLVAGDQVCLKYDHQGKEYVAWYEFSQRVLKDEEGNI
                     VYRKKRGETHLPELEKEAVLTLDEEKSNLLVESIRMHYTDKPMAIFMLTPDYDPSNHI
                     IVSIFLSQLYKELSTQCVKTKGDKCHRRIHFILDEFGNMPPMDNMEGIMTVTAGRNML
                     WDLFIQSYQQLYAKYGEQNAAIIKENCQTHIYIMSTDDNTIEEFSKKVGNKTVEQENS
                     TINSLGMNTHINRNVDSDRILTPERISTFLEGETIVLAPLKRQDLKGHKIRPYPIFNH
                     GETNLPYAYQYIEDFNPSWDINDIDIHCTHADLDLKALQIDYDYFLPDEYARNLYHGL
                     HDQLEEKNEENMFYKIVQSLENETAKKLLIQFYEEQAADRLQEYVEKLIKNKEISEMI
                     GKRLLLESEKVVKE"
     gene            13413..15944
                     /locus_tag="U2K71_001654"
     CDS             13413..15944
                     /locus_tag="U2K71_001654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315736.1"
                     /translation="MIIGGIFSGIADWWSGLFGEGDANPKTVTKVLQDYGNYLQYQDV
                     FSWIWNTFLWGLIKGLYNLNTLLEKTIYQSFDLKDLLNAAGVNALFQSLISKVLALFC
                     VVTLIYLGLKFFMSKHPPKVKNILVNLFMAMILIFGGSSLIDEGLNISKSFYGDVTAA
                     NKADKTGSPAFQIIQNNVIDVSTLLATDPNKVESLPTDKRNALTAKNFKTANINGIIT
                     PKQAKEMADKDKSGLSDLQKDRLRALQYRLELDDNGKEIPVQISDEGLAKYVYTSGYR
                     RYSSKPGIILAGESSLAVAYLFILFTIVTCIIELVFKKFYLVIAASTDFETGQRMKSA
                     IEDISQSFLLLAFTILELRIYTLMLSGIGDLHAAGKINGFLYVVALIVLTIALFKGSQ
                     SVTKIFGVDTSLKNGSNSLLSMFALGNIAKNVGSGAKNLASAGKSGLSHLNDIRKNGF
                     GHRKDVTPDNGEEQSDDKATTVPNSKKTVSEMFGKAKNGVSKAAEGLGYVNERGLKGT
                     AEDLTEKAKDTLEEKLDGTRAKELAEAVKNPSEALEKQKEKASEVKEGLKDAYGEGQV
                     NAAVKANTTTEKENQEGIVIPSVKDHQEALHSNENSIEEKNSIEERNSEEGKNGQLPK
                     TDPEQYRYKPNSDLENEKRISDLSKVNQEDKAENEGQLPKTDPEQYRYRSVNSENEKR
                     ISDLPKRNQEVQTDEDSIVLPNDNGIEVGTEKSTKIGLPKAQNLPMKDSRLNQFKEND
                     QSFVHQSTAGTVQMKPSRLPENGNLTTVSYNLSESGETGNASVQNVVTREAAPKPIQN
                     VHTGYKPANVAFTAPTKINVTRETKNRNNALKEQLLRDIKQDIKP"
     gene            15994..16326
                     /locus_tag="U2K71_001655"
     CDS             15994..16326
                     /locus_tag="U2K71_001655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315737.1"
                     /translation="MDKYINMKNPPRFSGEWKFFAFFFLDLLTLGGCFFWAKMVNNLV
                     HLPLFFALLNYGLWIAIALVIVWRPYNNPGQRQIFAIIESIFFADKNHYHALDPNRNY
                     HGTYKEDE"
     gene            16327..16920
                     /locus_tag="U2K71_001656"
     CDS             16327..16920
                     /locus_tag="U2K71_001656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315738.1"
                     /translation="MRVKGQNSVERKSILQFGKPEERSILDLFPYKSCTEEGVLITRE
                     DHFQRYYRVVSTDVEGLNEQEKLERMNQLTTVMRTYVPTIKLVSLTTETDLTEQIVQK
                     RQLLDKNRMEQSLGRNLQRLKKYEKKLVEEIQELKRAEQERPDLSFFFVIEGKTIKDL
                     QTRNRQLMRSSGILGLKPLYKKELIKILYRMNNMNDE"
     gene            17083..19107
                     /locus_tag="U2K71_001657"
     CDS             17083..19107
                     /locus_tag="U2K71_001657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase"
                     /protein_id="HEM7315739.1"
                     /translation="MLEAIKTAFSPQNDQKVLDPLKQLGNREIEILKSKGYDLDFLKQ
                     VMPQGGLNFDEEYVTYGNGYSRCVTVYHYTKNPTLFWLANLMNNPHTVATLDVSTDEK
                     QKVIDQINRALDELNDRSDNERKATDRNKSAEEYLELAEYARKLTSNGEISKQVKVRV
                     HVYGNTIQEVDERAQELIENLKGMDYRAVTYLHKTKRDWQSMFTMFDEQAEWFGNIKG
                     QSLPSLNIGGGFPFHHQTLKDPGGTQYGETMTGGPFVWNATYKNSVRLSTNTLLLGMM
                     GAGKSTVLKMIAEAHLAAGDFIWGFEKGKDFIPFLKEYNGIMVRLDGSDGMINPLEIF
                     ATRTYDEASSLYDDGSVKDLKINEAASYQTHLDKVVYQVQLVSPQLKGTMKAEFKTYL
                     NRFYEEYGTVPRGFTSSNSRSNTETQVTGKDPEAYPTFKEFLDFLGQLELPGASQEKK
                     NRKEEMESIVESLCETYGMIFDGHSTIRHLDQQQLVFFDIDSITGLDRGVQQCQMYMA
                     MTLIWNQALIQGRRQRRLIKEKQMEEQDFKRFLAMIDECHNAINPDFIETVNYVTNFQ
                     REMRKVEAMTVLATQSPQEMVPENMSSDNFSKLKKVFEFSSTKIFMRMDESILRHIEG
                     LVGKSMTNSELESIPQQNQGDAVINFGSKETIRVHFSPNKRQLKLFDGGK"
     gene            19107..20312
                     /locus_tag="U2K71_001658"
     CDS             19107..20312
                     /locus_tag="U2K71_001658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEM7315740.1"
                     /translation="MENQNESLIKQYVKRRAKRRLFLWVFGTSAGLITILITVFVTLF
                     LILAAGSIDNSDSDSSSGGEAFTGEYSEGLPIYKEIKGRGPFSDEIAQYAVGAAVKYK
                     LLPSVILSQYGYESAFGTSASARNDLNYFGITWFDGCLFPKGTARGIGGIEGGWYMKF
                     PNSKAAFSYYGFMVATQSNFNACVGNKSPGASLLILGRGGYAAAGITEDSPYYTGCMS
                     IITSNKLTEYDEFAIKHWGEGGNNNGTITGEWTNPFPGSSLDKSSFSGGQLFGTNPGG
                     EFRPNGFHDGLDFGSVDHPGSEIHAVHGGKVVYVGNPGISGLGACVIVINYDGLNMVY
                     QEFANSTGNSRVKVGDQVKVGQVIGIRDTAHLHLGFTRMDWRQAQGHAFTDDGTWIDP
                     LPFLNSSKK"
     gene            20328..20972
                     /locus_tag="U2K71_001659"
     CDS             20328..20972
                     /locus_tag="U2K71_001659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315741.1"
                     /translation="MENKTLKIVLTAAISVSLIGNIALFWQLKEEQETNTFKIEEVAK
                     NASEKLKKNSSEAQAKIDKLNQTINEKNQEIEQLKQQDGTKAKEEVTEAQSKTAEEFG
                     KLAIDKSLSRNELTEKLKDVATQEVIDKLSPADDDHQHDDYGSYSFTLGDVQTYAQTS
                     SVKEEQNFVVFVDYTIGNPQFKDVKPQKIKGGLTVTEKQVDGQWKVTDFSYFTR"
     gene            20983..21789
                     /locus_tag="U2K71_001660"
     CDS             20983..21789
                     /locus_tag="U2K71_001660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315742.1"
                     /translation="MSKKKGERQRFKKRKSYLRWLGIFMILGFLILASPIYTHQKVDI
                     PDEVSTNAFLNTKELTMVSKEKGANQQLVFEFAIDDEDQSLLKELANLNYRVEVKTAK
                     GDYQAIKAKVIKVSDDYFAVKLSHVPEEYIAMRLTIIPEKIDPKVDMQEPQDLIYYVH
                     EDKVKDQVKDEDYEQHAINYKVKGYKKEQKAAQKQMESFQATIDLNEELVKKLKDQLP
                     YQVADDQENTENKINSYQQEIETSKTQMKDQEEIIQKLQEKIDRMTKEKI"
     gene            21811..22041
                     /locus_tag="U2K71_001661"
     CDS             21811..22041
                     /locus_tag="U2K71_001661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315743.1"
                     /translation="MKRTNLADKDTFAEQKVLIKAYLKVVKAKEICREVNLSNTTISE
                     LKNGRKNLDTLKFRSFEALYVAAKKYFTADNK"
     gene            22555..22863
                     /locus_tag="U2K71_001662"
     CDS             22555..22863
                     /locus_tag="U2K71_001662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315744.1"
                     /translation="MEVRIRNVDEKIVREIDRRCDELSNRTGNKWSRNDYLKLLVEND
                     FDRPLMEYKQDKFDQLLNQLIDKFSFVQSHNTKVLEEYICQNNQIIELLIEQSRGGEV
                     "
     gene            22865..23287
                     /locus_tag="U2K71_001663"
     CDS             22865..23287
                     /locus_tag="U2K71_001663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315745.1"
                     /translation="MAKVIDKHIYFYETHIDVLDEIIATHAEVKNYSEAVRFLCMNYD
                     SEVEKKVNSNQAKLNAMGKEISILTEMVSEFADLHLKDTGILIGTNSTVYQEAKERVE
                     GKIKANQTKKYSPKIKKTISESVTTKSEVFDPTRFLKR"
     gene            23307..24896
                     /locus_tag="U2K71_001664"
     CDS             23307..24896
                     /locus_tag="U2K71_001664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315746.1"
                     /translation="MSKSPAVILTCQFTLPNKKSFSTYIDYMTREKALEEKERRTPEE
                     EQELRLIKNALEDFYIPEGETFSESKTGKEFSETSNEAQSLMKHGINFDKLEEQDFTK
                     YLSYMTRHYALEQKKVLTHSEQKEYQRLNQAIKKYQTEPHKKASGNLPGVFSMASDEV
                     KGSDLKEIKQTFQRGQQKGSIIYQDVVSHDHSYLEKIGLYDPKTDTLDEEGLKAAGRK
                     MMETLFEKEQLNETGYWMATIHRNTKHIHIHFAVVEKKNTRKPYTEIENGVSYIVPKG
                     KRRQATLDEMKTNYVHELERFSYEKNRMGMEKRNLLTRKSDLRNTLTKVVKEPVRYDR
                     HAMSLLTEVYKNLPKKRSEWHYGDENRTKLSPYTREKLDELTQYVLTKEPGYQEYIQI
                     SKALKEEAKSLYGESKRESKDAEKNALFDLKKRTGNAILTELKKQDSQIKPLIERMDQ
                     MQSIYNERSFRTLKQLPTNEYKYSYEEFMKRRKQFQQRVVTQRSLRQLNRTINAHEEK
                     YHASKAYEEQQRRIQMEQERQ"
     gene            24908..25243
                     /locus_tag="U2K71_001665"
     CDS             24908..25243
                     /locus_tag="U2K71_001665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315747.1"
                     /translation="MKQYLDQWKVIEGSLREERIEQLPDCLEKEHLFQIREMLRNEQF
                     DPNQFLVVEYSATGVYCCNHVKGEKYFIIQEYEGKLAPYYTTWEMNEEGINNFPCKSI
                     EESISLTEC"
     gene            25389..>25576
                     /locus_tag="U2K71_001666"
     CDS             25389..>25576
                     /locus_tag="U2K71_001666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315748.1"
                     /translation="MAYSTIGVVVEKSRDNLVFVTEIQTGRAFVVTDKAAKAYQSGDI
                     LTLNLTTKTFVDAAEDYP"
BASE COUNT         9545 a         3967 c         5254 g         6810 t
ORIGIN      
        1 atcaaataaa caagaaccgt aggaactacg gggatagctt ggtaaataag aggcacctct
       61 gttagtaaag taatacgcta tcaagtatgc tctcttccca agaagctccc acttcaagcg
      121 taagaaaccg ttaggtttag ctaagtggtg agtagttcac aatgacgctt acgaatgata
      181 atggagacga attatctgtt gaagatactt ggtcatattt gagtgattat attgtcagcg
      241 ttgaaatcat tgaatttgta gcggataaag aagaataaaa actggaggaa taaattttac
      301 agataaaggc ttagaagaag tcacgacgaa taataaaaag gtagtattac cttaggcatt
      361 gagtaaggaa ttcatttagg aggaaaatta gatggcgcga ccagttttag aggcgatgga
      421 tatggatact ttgacaaaag gaaggtatgt acagctgtta gctcgtggtt attcacctaa
      481 agatgtattc aaatccctgt ctaaaggaac agatatggaa aaagaaagat tacgaaaaga
      541 attcgattat tggagaaccc ataatggaat caaggatttg aaaccagcac ggccagtaat
      601 taaacgaaag aaaaagataa cccaagactg aagtgtagag cgtatgtgat cataggcttt
      661 tttatttttg caaaaaacaa ggaaggtagg aaaactcatg aattatgaag aaatcgaaaa
      721 cagaaaaaaa gtgtcgaaat aaatggaaga aaagttgcta aaaacgatga aacaaaaaca
      781 ccttaaaaga ttatctgtca tgcaatatat caatgatatg cagataacag gaaaagagaa
      841 agcttgtttg ttaggttcaa tgaaaaactt tgaacagttg agacgaactt atgtgaaaac
      901 aagttctaat tgtcaactat tgttagaagt ctcatgacat tcattaatag aattcatttt
      961 taaaactttt tctatggaaa tgagaaaaag attttttgtt ttgtctgaaa gggggtagct
     1021 ggatgagaaa atggttattt ggttttttag gagtcgcttt aatcgtcttt tgcagtgtgt
     1081 ttggctatgt tagctaccaa aaacatgaag gagaggtgtt taagcaaaat atagaaaaaa
     1141 agatgcccgt agatcaaatc aatgcacatg caaagagtta taaggaggat gcaacgaacg
     1201 tcaataatga tatgtctttg gggcaaatgt tgtccataca acaagaagca atcgaaatgg
     1261 gcgtcaataa acaagtattt gcacaaattc agattcctgc acttggttta gcattgccaa
     1321 tttttaaagg ggcaaatcaa tacactttga gtcttggagc ggctacttat ttttatgaag
     1381 atgctgaaat gggcaaagga aactatgtcc tagcaggtca taacatggaa atgccaggtg
     1441 ttttattttc tgatattcaa aagttgtcat taggggaggt gatggactta gttagtaacg
     1501 atggcgtcta tcgttacaag gtaacgcgta aatttatcgt acctgagtac tttaaattaa
     1561 tcgatggcgt tcctgaagag aactctttct tgtctttgcc aaagaaagga gagaaaccct
     1621 tattaacgtt gtttacttgt gtttatacgt cacaaggaaa agaaagatac gtggtacagg
     1681 gagaactaca ataacaaggt tttaatcaac gtgagtcgtt gaaatatgaa atgaggagag
     1741 taaaaaatgg caataaatcg gtatgtaaaa aaagctggaa tgcttattgg aatgttagga
     1801 atcattgcag gctgctttca gggatatcga gcatatgcag aagatgtcac gcaaaaaacg
     1861 ccaccagaga aggtcaatat cacggttcat aaattaatgt acgatcaagg aacgcaattg
     1921 aatgttgata ttgacgggat caaaaacgat ggttatacgc acgatcaata tcctacaggt
     1981 gtaacgaaat acaacaaggc agattatggt gatgttgagt ttacgttagg aaatattacg
     2041 gatcaagtat taccgagaga agattctgat ctaaccaacg cgaaagtgga cgagattgta
     2101 caagatgtag aagacaaggg atctaacagt gagtacgtca agaatgcaac caacaaaatt
     2161 acttcagctg tagatgaaaa tggtgagatc acttttactg atcaaccagc ctatgtcaat
     2221 tccaaaggga atgtatatgt ggtttatgaa agtaaatccg ctgctggatt agtcacacaa
     2281 aaagcaaaac caatggttgt gattgcgcca atgacagata aaacaggatc aggattttta
     2341 aaaaatattc atttgtatcc taagaacatt gtttctaagt tgagttttga attaacgaaa
     2401 tttggtgacg atggcgcaga ccaaagcaaa caaacccttt taaaaggggc aaaatttgag
     2461 ttatacaagg gcgaacctgg aaaaggaaca aaattgggtg atcttgtatc ggatgatcaa
     2521 ggaaagctga ctgcgactga tttgacgtta ggaaaatact attttgtgga agttccttcg
     2581 gaagttgtag tgggatcaga tcaagaacca acagcagatc aatatttatt aggcgcagac
     2641 gctcgcaacg atgcgcacaa taaattaacc tttgaaatta cgaatgatgg cgtgacaagt
     2701 gacttaaaag cttcatacgt aaactataaa gcaccagtga tcgataaaac agtaacaaat
     2761 ggaacaggac aagaacattc tttccaaatt ggtgacgcag taaattatca aggaacgatt
     2821 catattccaa cagacattgc aggtggcgca gacggtatca cagtaaacgg tgtgaaaagt
     2881 gaaacttctc cttacagtgt atttaaatgg ggagatacag caggacaggg actttcttat
     2941 gtcgctgcta aagcaaatat caaagtaaca aataaagatg gatctgtggt attaaaagaa
     3001 aatacagatt ataaaatcca aaattcagaa aatggctttg tgatcgattt tatcgtgaat
     3061 aatggccaag taagtgatac tgtagcaagt ttacacggac aagatcttca aatgacttat
     3121 aacatgtatg taaatgacag cgcagctgtt gccaatccat taacaaatag cgtggatttc
     3181 gtttataaca acaatccatt taatcaggaa gaacaccacg aaaaaacgaa agctgacgtt
     3241 gtgacttatg gtgcaaaatt cttaaaagtc gatagcggac tatttggaac aggaatcaaa
     3301 gcaacaccgc ttgaaggcgc agaatttgct gctaaaaacg cagaaggaaa atattatggt
     3361 ggattagttg atacggataa agacggtgtc aaagaagcag tatgggtaga cgatgtagca
     3421 aatgcagcga tcctaaaatc agataaagaa ggtcactttg aaatcactgg tttaactgaa
     3481 ggtgaatatt cactggaaga aacaaaagcg cctgaaaact atcaaaaatt aacgaaagag
     3541 atctctttca aagtagacaa agattcatac aaagaagaaa accgaatcac gatcaaaaac
     3601 aatcaaaaag cttccgtacc aatgaccggt agcaacggtt ttcaaacgta tgtattaatc
     3661 tcttgcttgt tgctaggagc aggggcactt tctgcagttg tttactttaa gaagaaagcc
     3721 tagaaggatc aaaaccaatg attatcagga agcctaagaa aaagcgaatc atgggtaaat
     3781 ggatcattgc tttctggtta ctttcagctg taggtgtctt acttttgatg cctgctgaag
     3841 cgagcgtggc gaaataccaa caaaatcagc agattgccgc aatcgatcga acaggaacgg
     3901 cagcagaaac ggattcttct ttggatgttg ctaagattga gttaggcgat cctgttggta
     3961 ttcttacaat tctaagtatt tctttaaaac taccaattta tgacggaacg tccgacaaaa
     4021 ttttagaaaa tggtgtaggt attacggaag gaactggtga tattacagga ggaaacggta
     4081 aaaatccttt aatcgcaggt catagtggac tttataaaga taatcttttt gatgatttac
     4141 cttcagtaaa aaagggagaa aagttttata tcaaagttga tggtgagcag cacgcttatc
     4201 aaatcgatcg gattgaggaa gtgcaaaaag acgaactaca aagaaacttt gtcacttact
     4261 tagaatcaaa tcccaatgaa gatcgagtaa cgctaatgac atgtacgcct aaggggatca
     4321 acacacaccg ttttctagtg tatgggaagc gagtaacctt tacaaaatca gaattaaaag
     4381 atgaagaaaa taagaaacaa aaattatctt ggaaatggct tttaggaagt actgtttttc
     4441 tttcagtcat gatcattggt tcgctatttg tttataaaaa gaaaaaataa aggagggtaa
     4501 ccaacatgag aaatagaaaa tggctatttg tttgtgcagc cgctcttttt tctttcactg
     4561 cctacacaca gttgacgttt gcagacagac aagaaagcac ggatagcaca gaacaaacaa
     4621 tccaaacgtc agatagcagc acgattcaaa gcagcgatga aaccgtcgat tcgtcaacag
     4681 ttgattcttc agaagaacct aaaattgctg aagacgatca aatagtcaaa cagaccattc
     4741 acgttcaaaa gattatttct tcagaacaat tagacaacca ggggcatgaa aaacaagaag
     4801 gagtaaatgg cgcgaagttt tctgtttatg atgtatcgga cattttacaa aagatggatg
     4861 ttaaggatct cactactgac caaattgaat cccaattaaa aaatcgagtg aaaaaactat
     4921 ccagtgatca attgaagcta gtttctaatg aagaaacaaa gacaattgat caacaaacag
     4981 gtgtctttga attctcggta gaagtacagg caaatcagaa acaagcttat tatattgtca
     5041 atgaatcaag tcctgaaaac atttctaata gcgaggatat tttgctatta acacctgttt
     5101 ctgataaaaa cggtgaattc ttaaaggatg tgtggatcta tcctaaatca gaagcttcgc
     5161 aacctaaaga agaagtaaaa aaaatcgttt ctactggcgt aaagaaaaac ttctttgaga
     5221 attgttggga ttttgttact cacttgtttt ctagagatta aggaagaaat aaaatgaaac
     5281 tgatcaaatt attattattc aatttttctt gtttaaccgc agtctttttg tactgcggtt
     5341 tattattttc taaactatct attttgccaa gctaccaact atacctaatg aggattttaa
     5401 acataccagc tgcttccatg ccgctagata ttatggaaaa gctcattagg aatccattat
     5461 ttactacgat caatttgatc gttattgtcg gaatttcact agtcatttat aaaaaagagg
     5521 ggatgaaggg atgaatttta aacactcgtg gacaagacgg ttaaaatatg tttttcccat
     5581 aatgatgatg ttaggtacat tgtttgggtt aaaaacagcc aatgtctttg ctgaaaaagt
     5641 tattgtcgat ccgtcaaatc caatccagaa cgtaaagaat aattttatta ttccaaaaga
     5701 tgttcctaat gcaaaaacaa atattactgt aaacggagcg tcaaaagttt acaaatatca
     5761 tgttgatgcc aacaaaggtg gttttacaga cgactacgta ggattagtgg agggaaacaa
     5821 aaatatacgc tatccttctt tccaaaaaga atacggtgaa acaaaccttg ttttagaagg
     5881 cgtaacaacc aacaccaaag tcaatattga ctatggaaaa atcggtacgt ataacgggaa
     5941 aaaagtcaat atcaagctag ttctatctaa tattcactta tactcggata cgttgccgtg
     6001 gaatctacta gataacaact atacaaaaac tcattttcga gacgatggct ataaaaatac
     6061 gaatggtgca atgagtaaat ctaaaaaaag aacagtactt tggatctcag acaatctttt
     6121 ctcaggaatt gtttaccatt caacacaaat gaatgtgcag ctagtagcca catatgagga
     6181 cggtagcccc gttcaatttt caggagatac atttatcagt tttaactctt tgaaccctgc
     6241 aggaggaaaa agcacggatc ttaaaggaga atatgcccac tatgacaaaa tgaatacgac
     6301 agattggtat gtcagaaaag atacggttct ttcagaattt aaatctttct acaacaatct
     6361 gaacgtggta ggtggccacc caggaggaag ctctaagctg acacaagcgg ataatgattt
     6421 caataatttg catgataaat taggagatcc gaagtttggt caaggtactg tgtcctttaa
     6481 aatatctgaa gcaaatccga cgtttgtgat tggttcttct aatgttcaaa cctggtttac
     6541 tttatcttct gcgacaatct ttagtgttgt tcctgatcaa cctgaaaaaa caggggttga
     6601 taaaaatgga aataacgtga acgataaaat gttgcaagta ggagacacga ttcaatatcg
     6661 aatcaaacag aaggtgaatc gattaggagt ggatttacta gcggtttacg atcgatttga
     6721 gttaatcgat aatttaccaa aagaagtaaa ttacgtcgat gcacacgttg aaagtggtac
     6781 aaataaaaaa ttcgatgtat ctggtgaagt gacctatgac aaaacaaaac accaggtaaa
     6841 atatgcagcg aaagctgata cattaaaaaa tcgaatgaag tataacggcg aaacttacga
     6901 attagtgatc aatgtaaaag tcaatgaatt ggccaatcaa aatagtgtag ccaaaaatca
     6961 gggaacgtcg atcattaata aagtagaaaa ggatacaaat atcattaccg tttatttccc
     7021 taaaattcca gtgaaagaag tacaacaaaa tggaaaagat gtaaacggac gaaatgacgg
     7081 taagaagggt acgccgacag ctcctttaaa cgcaggatct gaagtacaat atctagtcac
     7141 acaaaaatgg cacacaaaag gcgtagacgc tgcgtcagat cactacaaac aatttagtat
     7201 tcaagatcca attgaagctc gtttgacata taaagaaggt tctgcgcaag taatagataa
     7261 gagtaaagga aaagatatta cttctgaagg cacactaacc tatgatagca attcaagaac
     7321 gctgaagtgg gaagcttccg ctgacttctt aagtaacaat cttttagatg gacgagaaat
     7381 tcagctgata tttacagcta aaactccatt acaatcagaa aaaaatattg ataaccaagc
     7441 cgtagtagca gtagataatg tttctaataa aacaaatgtt gtgacaattg gggttgatcc
     7501 taacttgcca caggtcattg ttcctaaaac aggttctaca catttagtaa cgatttcagc
     7561 ggtcagctta ctattacttg tgttagcaac ttttagttat gcggttctga gatacattta
     7621 aaataaaaaa acgaaagaat gaataaaatg aaaaagttaa taatagtagt tacttccttg
     7681 attgccatga ttgttatttt agtcggatgt agtaatgaaa aaaataaaca aactaatcaa
     7741 gataatggcg tattgaaatc aggaactatg tggaaagaag aagtaggtgg tcttgtttat
     7801 aatttgaaaa ttatagacga gactacctgg gaatactctg agagtgtatg gcatccagat
     7861 cctgtacaaa taacagtcaa gaggcaaaaa gactataaag gtttagaaag atacaaaata
     7921 gtagattcag ctggtgttag ggaatttata aacaaatctg attctttatt tattgttgtt
     7981 ccctatgaga agaatggcgt aaaaaaaata atttttcttg gatctagcaa ggatgaaaag
     8041 cgaacgaaag agaaattgat tcacgatgga tcacaatcca ataaatataa gttacaaaag
     8101 actagcgagt gattgctagt ctttttattt gtcctgaagg aggaatcaca gtggcaaaag
     8161 ctaaaacgcc atttgaaaaa gccgcgtcta aaatcttaga acgccgcggt gtatctgaaa
     8221 cacagtggaa acaagaagtc cttgaaaaag cacaatttga cctattacgg ggaaaagaca
     8281 aggacttaga aggatatatc tatcaacgtg aacgtgaaaa gcttgttttc gatgaaatta
     8341 ttaaacacta aaaggagcga acaattttat gtttgaatca attacaatga attttttaac
     8401 acaaccgaaa gtagtagctg cggttgatat ttttagagca ttacaaactg ccgcaactag
     8461 tttaaaaaac aatctgatga tttttgctgc tgctgcggcc gttgcttcca ttgttattgg
     8521 gggtcttttc ttcctatttg gacgaagagg agctgaaaat gggaagatgc acgtaggcta
     8581 tgtattgatg ggggtaggag ttattttagc agcagcttct attgtggttt cgttatttgg
     8641 attgtttgga caaacacctc aaggattata agaaagtaaa aaagtaggag taagaagtag
     8701 tcgatacttt ttactccttt tttatgaaag gagaatactc atgtacttta cgattgcagg
     8761 caataccaaa acagataaag agttaataaa aaaagcaacg aaggaagtag gaaagcgtac
     8821 ttctttttta ccgaaaacaa ttaaatattc tgtcattgaa acggaagaac agctacatgt
     8881 gttagggaaa gtactggaac acgaaagtcg atcttctgtt aagttgactc tcacgttaca
     8941 tgacacagaa ggaaaaatag agaaagctgg tacagaatac gtttttggcc cactggatta
     9001 tcaaatttca gaaggtttct atcctacatt gatggattta atccgccttg atgtgtttaa
     9061 agaaacggat gaaactatga gtgtggaaga gtcttatcga aacgaaaaac ttttacaagt
     9121 tttagaagaa gagttagaga gcgaccaaag aaaagtgaat caagggagtg aagtcgaagt
     9181 aaatgaagaa gtaccatggg aacgaccaat atttccaaag aaggagtcaa aagaaaaaga
     9241 gacagtctat gttgacgaac aagctgatat tccgactcag aaatcgatat ttgaggaaga
     9301 gaacgatgtg atgggtcaat tggaggtaga tccctttgaa gaaacaatgg aggatgaaga
     9361 gacggccgaa gtagaagttg atccagttga agcgaatgaa gagtcaacat ggactgaagg
     9421 ggattctcag gaatatattg aaccacttat cagtgaagaa gtagaaaaaa acgaaataga
     9481 gatacaaaag gcaactgtat ttgattatat tttggatcaa tatccgccag agcatgaatg
     9541 gatgaaagaa aaaaacaaat tatttttaaa tgagctttat tcgaaatatc aattacccca
     9601 aacctatcaa gcatttttag agagtaaaga agaattaatc tcacagggta aaggcacact
     9661 tttagaacag ttagagtctg tccaatcaac aaattggcat gagcttgcac aagaagattt
     9721 attagccacc tttgaagaac gtcaacaaga aacagatcaa gaaattaatg agtatgcgac
     9781 ggaacagtcg tctaattggg agaaagcaaa agaggaacta aataaagaag aacagcgagt
     9841 gattgaagaa gaagtacaaa aaattaaaga gcgatacgaa aagaaacgcg acaatgcgta
     9901 ttccttgtgt gtagcaaaga tcaataattt tactcagacg aatcaagaag cgttatcgaa
     9961 agaaaaagaa aacttgttaa aagaacgtat taaagaacga aaagaagaat tttaccgagg
    10021 tttgcgtaca gataagctaa agattagcaa agaattaaat gaacaactga ataagctata
    10081 tcaaacaacg actgaagttc ttatggaaaa atacgaaaag atccagcaag aattacaagt
    10141 gcaaattcca aaatggaaaa aagagtatga aattcaacaa caagaattga aagaacaaga
    10201 agaaaaacaa cggcaaaaag aaaaagagcg cgaacaatta gaactaaaaa aacgtgaaca
    10261 ggatcttgcg gaacaacaaa tggcaataga aaaacaacgg ttaaaccaag aaaaggaaaa
    10321 agaagcagct gctcgtaagg aacgtcaaga acaaatggat acattgcgac aagctcagtt
    10381 aaattatcta gcacaacctc aaccaacgcc ttcagtagct gctgttcaaa ccccaatgaa
    10441 caaccaaaca aatctaactc cttcaccaca gcgttccttt aacggatgga tggtgggatg
    10501 tattgcaagt ttaagcttac ttctaggtgg aggaacagta tttgctttta atcatatcaa
    10561 tcaatcttca caggctgatt ctgtggccac aacgacgtca atcaaggaag aagcccaaga
    10621 acaagcaaga aaagagtatg aacaacaatt agaagaatta ttgaatcgta ccaatcagtc
    10681 aacaacagag tcaagcatgg aaaatagcaa agagagtgat tctagtaagg ctaaagaagg
    10741 atcaagcgat tcaactgtac aatcaaacac ttccgaaagt tcagaatcaa atgaggtgaa
    10801 ataatgcgtg ttagagaaac aacgaagcga aaagacaaac gattcaattt agaaaagaat
    10861 aggtatcttt ctcatttcta tcgctatact ccctcaggag aagagctacc agaaaaacat
    10921 gacaaacgca tttcgaaaaa gattttttat agtggacttg tatacctccc tttagagtat
    10981 ttagtaacaa gagtaccaga agttttcaac cgattggaaa ttgttaaggg aagcttacaa
    11041 ggaataaatc tgtttcaatt acaggaaatg ccacaggtct ttcattttga ttcagctggt
    11101 ttttggaatc catttactcc tacaactagt ttgggtacac gtttagcaat ttcgacagtt
    11161 tgtgtggttc cagctgtact tatgggattg aaaatggaca tgtggtttcg tcctctagca
    11221 gatggacaaa aaggggataa tcgattaatg accttaaaag aaatccaaca gatttatccg
    11281 caaatacctg aaacaaaaac tacttttcca ggttatggag gtattccaat cacacattac
    11341 aaaaaatatt ggtacattca aacagatact gttaatacgt gtattgttgg tacctctcga
    11401 tcagggaaag gtcagattga agtgttagcc accattgaca atttatctcg tgcagaaaaa
    11461 caaagttcaa tggtcgttaa tgatccaaaa acagaattat atgttggtag taaagatgaa
    11521 ctagaagcta gagggtatga tgtatatgcc tttaatatca ccgatccact gcaatcaatg
    11581 agtgataatc cattggcact catcgtgaaa tactggaaac gaggagacat agatactgca
    11641 acacagttaa ccaatacttt cacaaatacg atttattatg atgccaacgc ttcagataac
    11701 aaatacttta acgaaaacgc gcagaaagct gtcaatgcgc ttatttttgc gttgttagaa
    11761 caagctgatc aaacaggaga ttactccaaa ttgacaccga ataatctcgt tgagcttctt
    11821 tctgagattg gcggttttaa ttattatcca gatccagaca atgaattcaa acagttgaat
    11881 gccttagatg aatttatgaa tcagttgcca cctggaaaca ttgcaaaaaa acaatatggg
    11941 gcaacgaaaa ttggatctga taaagcgaag ggaaatattt tatcaacggc aatcacggga
    12001 ttgaatccat ttactctaac gaaaattgca aagatgactt ctcaatcaac gattgattat
    12061 aaatcggttg ggtttccaaa atacttagat ttgaaattac atgaaagtct tttaaatcaa
    12121 cgagtcgtag tagaatttta tcataaggga aagaaatttc atgaagaacg tgtcaaagta
    12181 ggctataaag gattttgtga aatcaatttt gattgtcatt tagttgcagg tgatcaagtt
    12241 tgtttaaaat acgatcatca aggaaaagaa tatgtggctt ggtatgaatt ttctcaaaga
    12301 gtattgaaag acgaagaggg taatatcgtt tatcggaaaa aacgaggaga aacccatttg
    12361 ccagaattag aaaaagaagc cgttctaaca ttagatgagg agaaaagtaa cttattagta
    12421 gagtctattc ggatgcacta taccgataaa ccaatggcta tttttatgct tacacctgat
    12481 tatgatccat ctaaccatat cattgttagt atttttctta gtcagttata taaagaatta
    12541 tctacgcaat gtgttaagac taaaggagat aaatgtcacc gcaggattca ttttatttta
    12601 gatgaatttg ggaatatgcc accaatggat aatatggaag gaatcatgac ggtcacagct
    12661 ggccgaaaca tgttgtggga tctgtttatt caatcttatc agcaacttta tgcgaagtat
    12721 ggagaacaaa acgcagcgat tatcaaagaa aattgtcaga cacatattta tattatgtcg
    12781 acagatgata ataccatcga agaattttct aaaaaagtgg ggaataagac ggttgaacaa
    12841 gagaacagta ccatcaattc attaggtatg aatactcata tcaatcgcaa tgttgattca
    12901 gatcgtatct taactcctga aagaatttct acttttttag aaggtgaaac gattgtactc
    12961 gctcctttaa aacgacaaga tttgaaaggt cataaaatac ggccgtatcc tatttttaat
    13021 catggggaaa cgaatcttcc ttatgcttat caatatattg aagatttcaa tccatcgtgg
    13081 gatatcaatg atatagacat acattgtaca catgcggact tggatcttaa ggctttacaa
    13141 attgattatg attatttcct accagatgaa tatgctagaa atctctatca tggactgcat
    13201 gatcaattag aagaaaaaaa cgaagaaaat atgttttata aaatcgttca atctcttgag
    13261 aatgagacgg ctaaaaaatt gttaattcaa ttttacgaag aacaagcagc agatcgactt
    13321 caagaatatg ttgaaaagtt gatcaaaaat aaagaaattt ctgaaatgat tgggaaacgg
    13381 ttgttattgg aatctgaaaa ggtggtgaaa gaatgatcat tggaggaatt ttttctggca
    13441 tagccgactg gtggtcaggc ttattcggag aaggggatgc aaaccccaaa accgtaacga
    13501 aagtcttaca agattatggt aattatctgc agtatcaaga tgtatttagt tggatttgga
    13561 acacttttct gtggggattg attaaaggat tatataatct caatacttta ctagaaaaaa
    13621 ccatttatca aagctttgat ttaaaagact tattgaacgc cgctggggta aatgcactat
    13681 ttcaaagttt gattagtaag gtattggctc ttttttgtgt cgtcacatta atttatttag
    13741 gcttgaaatt ttttatgagt aaacacccac caaaagtcaa aaatatcttg gttaatttat
    13801 ttatggccat gatcttgatt tttggtggga gttctttaat cgatgaaggg ttaaatatca
    13861 gtaaaagctt ttacggagac gtcactgcag caaataaagc agataaaacg ggttcacctg
    13921 cttttcaaat tattcaaaat aatgtgatcg atgtcagtac attactagca actgacccga
    13981 ataaagtaga atccctgcca acagacaaaa gaaatgcttt aacagcgaaa aattttaaaa
    14041 cagcgaatat caatgggatt attactccta aacaagctaa agaaatggcg gataaagata
    14101 agagtggttt atcagattta caaaaggatc ggttaagagc gttacaatac cgattggaac
    14161 tagatgataa tgggaaagaa attcctgttc aaattagtga cgaaggctta gcaaaatatg
    14221 tctatacttc agggtatcgt cgatacagta gtaaaccagg tattatctta gctggtgaat
    14281 cttctttggc ggtggcctat ttatttatcc tatttacgat cgttacttgc attattgagc
    14341 tggtatttaa aaaattctat ttagtgattg ccgcgtcaac ggactttgag acaggccaac
    14401 ggatgaaaag tgcgatagaa gatatttctc aatccttctt gttattggcg tttacgattt
    14461 tagaattacg aatctataca ttaatgcttt caggtatcgg tgatttgcat gcggcaggaa
    14521 agatcaatgg attcttgtat gttgttgctt taattgtgct aactattgcg ctattcaaag
    14581 gctctcaatc ggttacgaag attttcggtg ttgatacgag tctgaaaaac ggcagtaatt
    14641 ctcttcttag catgtttgcc ttaggaaata ttgcgaaaaa tgtgggtagc ggagctaaaa
    14701 accttgcaag tgctggtaag agtggtttaa gccatttaaa tgatattcgt aaaaatggat
    14761 ttggccatcg aaaagatgtt acccctgata acggggaaga acaatcagac gataaagcta
    14821 caactgtacc taatagtaag aaaacagttt ccgaaatgtt cgggaaagcg aaaaatggtg
    14881 tttctaaagc cgcagaaggt cttggttacg tcaatgaacg tgggttaaaa ggaacagctg
    14941 aagatcttac agaaaaagcc aaagacacct tagaagagaa attagacggc acgagagcta
    15001 aagaattggc tgaagctgtg aagaatccat ccgaagcact agaaaaacaa aaagaaaaag
    15061 cttcagaagt caaagaagga ctaaaagatg cgtatggtga aggacaagtt aatgcagcag
    15121 taaaagctaa tacaacaacc gaaaaagaaa atcaagaagg tatagtgatt ccttcagtca
    15181 aagatcacca agaagcgttg cactctaatg aaaattcgat tgaagagaaa aattccatag
    15241 aagaaagaaa ttcagaagaa ggaaaaaatg gacagttacc aaaaactgat ccagaacaat
    15301 atcgctataa acctaacagt gaccttgaaa atgagaagcg aatttcagat ttatcaaaag
    15361 taaatcaaga agacaaagca gaaaatgagg gacagttacc aaaaactgat ccagaacaat
    15421 atcggtatag atctgttaac tccgaaaatg aaaaacgcat ttcagattta ccaaaacgaa
    15481 atcaagaagt acaaacggat gaggattcca tcgttctgcc aaatgataat ggaatcgaag
    15541 ttggaactga aaaatcaacg aaaattggtt tacctaaggc acaaaattta ccaatgaaag
    15601 atagtcgttt gaatcaattc aaggagaatg atcaaagctt tgttcaccaa tcaactgcag
    15661 gtactgttca aatgaaacca agtcgtttgc ctgaaaatgg aaatctgaca actgtttctt
    15721 acaatttatc tgaaagtggt gagacaggga atgcgtccgt tcaaaatgtt gtgacaagag
    15781 aagctgcacc aaaacctatt caaaacgttc atacaggtta caagccagca aatgtggcat
    15841 ttactgcacc aacaaaaata aatgtgacaa gagaaactaa aaacaggaac aatgcattga
    15901 aagaacaact attgagggat atcaaacagg atatcaaacc ataaaaggcc tacaagttag
    15961 gccttttatt tatatgaaaa ggagaaatca attatggata aatatataaa catgaaaaac
    16021 cctccaagat ttagtggaga atggaagttt ttcgcttttt tctttttaga tctattaacg
    16081 cttggtggct gctttttttg ggcgaaaatg gtcaataatc ttgtccactt accactcttt
    16141 tttgcactct tgaattatgg cttatggatt gcgatagcgt tagtgattgt ttggcgaccc
    16201 tataataacc caggtcaacg acaaattttt gcgattatcg agtctatttt ttttgcggac
    16261 aagaaccatt atcatgcgtt agatcctaac cgaaattatc atggtacgta taaggaggat
    16321 gaataaatgc gagtcaaagg acaaaattca gttgaaagga aatccatttt gcagtttgga
    16381 aaaccagaag agcgctctat cttggactta tttccgtata aatcctgtac agaagaggga
    16441 gtcttaatta ctagagaaga tcattttcaa cgttattatc gtgtagtttc tacagacgtg
    16501 gaaggattaa atgaacaaga gaaactagaa cgaatgaatc agttgaccac cgtcatgcgt
    16561 acgtatgtgc caacaattaa gttagttagt ttgaccacag agacggatct aacggaacaa
    16621 atcgtgcaaa agcggcaatt attagataaa aatcgtatgg aacagtcctt aggaagaaat
    16681 ctacaacgat taaaaaaata tgaaaagaaa ttagtcgaag aaatccagga attgaaacga
    16741 gcagaacaag aacgtcctga cttaagcttc tttttcgtca ttgaaggaaa gacaatcaag
    16801 gatttacaaa ccagaaaccg tcagttaatg cgatctagtg gcattttagg gttaaaacca
    16861 ctctacaaaa aagaattgat caagattctg tatcgaatga acaatatgaa tgatgagtag
    16921 gaatggagaa gagagaatga aatatccgtt gatatggtta gaaaaagatg ttccgaagga
    16981 agagaccact agaaaatttc ctcacacttt aagcctgggt aagttgggaa aagggcaatc
    17041 ttttaaccaa gtaaagaatc aaacaagaag aggtgactgt acatgttaga agcaattaaa
    17101 acagcttttt caccacaaaa tgatcaaaaa gtacttgatc cattaaagca actaggaaat
    17161 cgggaaatag aaattttaaa atccaaaggg tatgatttag attttttaaa acaagtgatg
    17221 ccacaaggtg ggttaaattt tgatgaagaa tatgtgacgt atggtaatgg ttattcacga
    17281 tgtgtgacag tttatcacta cacaaaaaat cctacgcttt tttggttggc caatctcatg
    17341 aacaatcctc atacagtggc cacactcgat gttagcacag atgaaaagca aaaagtgatt
    17401 gatcaaatca atcgtgcctt agatgaatta aatgatcgat cagataatga acgaaaagcg
    17461 actgaccgaa ataaaagcgc agaagaatac ctggaactag cggaatatgc tcgtaaattg
    17521 acttctaatg gggaaatttc taagcaagtc aaagtgagag tacatgttta cggaaacacg
    17581 attcaagaag tagatgaacg agcgcaggaa ctgatcgaaa atctaaaagg gatggattat
    17641 cgtgcggtga cttatctgca taaaaccaaa cgagattggc aatcgatgtt taccatgttc
    17701 gatgaacaag ctgaatggtt cggaaatatc aaagggcaat cactaccatc gttaaacatt
    17761 ggcggaggtt tcccgttcca ccaccaaacg ctaaaagatc caggagggac acaatatggg
    17821 gaaaccatga caggcggccc atttgtctgg aatgcgacgt ataaaaatag tgtacggttg
    17881 tctactaata ccttgcttct aggaatgatg ggagctggta aatcgaccgt tcttaaaatg
    17941 atcgcggaag cccatttagc tgcaggagac tttatttggg gctttgaaaa gggaaaagac
    18001 tttattcctt tcttaaagga atataacggt attatggtgc gtttagacgg atctgacgga
    18061 atgatcaatc ctttagagat atttgccaca cggacgtatg atgaagcatc cagtttatat
    18121 gacgatggat cagtcaaaga tttgaagatc aatgaagcag cgtcctatca aacacactta
    18181 gacaaagtgg tctatcaggt acagttagtg agtcctcagc taaaaggaac gatgaaagca
    18241 gaatttaaga cctacttaaa tcgtttttat gaagagtatg gaacagtacc acgcggcttt
    18301 acttcctcaa attcgcgttc aaatacggag acgcaagtca caggtaaaga tccagaagct
    18361 tatccgacgt tcaaagaatt tttagacttt ttaggacaac tggaattacc aggtgcttcc
    18421 caagagaaaa agaatcgaaa agaagaaatg gaatccatcg tagaatcctt atgtgagacg
    18481 tatggcatga tttttgatgg gcatagtacg attcgacacc ttgaccaaca acagttggtc
    18541 ttttttgaca ttgactctat tacaggctta gatcgagggg tacaacaatg tcaaatgtac
    18601 atggctatga cgttgatttg gaatcaggca ttgattcaag gaagaagaca acgacgttta
    18661 attaaagaaa aacaaatgga agaacaagac ttcaaacgct tcttggcgat gattgatgag
    18721 tgccataatg cgattaatcc agattttatt gaaacggtaa attatgtcac aaactttcaa
    18781 cgagaaatgc gtaaagtaga agcaatgact gttttagcta cacaatcccc acaagaaatg
    18841 gtgccagaaa atatgtcttc tgataacttc tcgaaactga agaaagtctt tgaattttct
    18901 tctacgaaga tctttatgcg tatggatgaa tctattttac gtcatataga aggattagtg
    18961 ggtaaatcaa tgacgaactc agaattagaa tccattcctc aacaaaatca aggagacgct
    19021 gtgatcaatt ttggctcaaa agaaaccatt cgtgttcatt ttagtccgaa caaacgtcaa
    19081 ttaaaattgt ttgatggagg aaaataatgg aaaaccaaaa tgaaagttta atcaaacagt
    19141 atgttaagcg tcgtgcaaag cggcgcttat ttttgtgggt gtttggtact tctgcaggtt
    19201 tgattactat tttgatcact gtgtttgtga ccctatttct aatacttgcg gcaggttcga
    19261 ttgataattc ggattcagat tcaagcagtg gtggcgaagc ttttacagga gaatattcgg
    19321 aaggcttacc gatttataaa gaaatcaaag gaagaggccc tttttctgat gagattgcgc
    19381 aatatgctgt aggtgcagca gtgaaatata aattattacc aagtgtgatc ctttcgcaat
    19441 acggttacga atcagcgttt ggtacgtcgg cgtccgcaag aaatgatctc aactattttg
    19501 ggattacgtg gtttgatggg tgtctgtttc ctaaaggaac tgcgcgagga atcggtggta
    19561 ttgaaggtgg ctggtacatg aagtttccca actcaaaagc tgcattttcc tattacggat
    19621 ttatggtagc cacacaatcc aattttaatg cttgtgtcgg aaataaaagc cctggcgcaa
    19681 gtttattgat cttaggacga ggcggttatg ccgctgcagg aattactgaa gatagtccgt
    19741 attatacagg ttgtatgtca attatcactt ctaataaatt aaccgaatat gatgaattcg
    19801 ccatcaaaca ttggggtgaa ggtggcaata acaatggcac aattactggt gaatggacga
    19861 atccattccc tggcagcagt ttagataaaa gttccttcag cggtggtcaa ttatttggaa
    19921 caaatccagg cggtgaattc cgtccgaatg gcttccatga tggattagac tttggttcag
    19981 ttgatcatcc aggaagtgaa attcacgcgg tgcatggagg aaaagtcgtg tacgttggca
    20041 atcctggtat ttcaggttta ggtgcatgtg tgatcgtgat taattacgac ggtttgaata
    20101 tggtatatca agaatttgcg aatagtacag gaaattctcg tgtgaaggtt ggcgatcaag
    20161 tgaaagttgg tcaagtaatc gggatacgag atacagcaca cttgcattta ggtttcactc
    20221 gaatggattg gcgtcaagca caaggacacg cattcacaga tgatggtaca tggattgatc
    20281 cattaccatt tttgaatagc agcaaaaaat agatcaggag aaaaaatatg gaaaataaaa
    20341 ctttaaaaat cgtcttaaca gctgcaatta gcgttagttt aatcggcaat atcgcgttgt
    20401 tttggcagct gaaagaagaa caagagacaa atacctttaa aattgaagaa gtggctaaaa
    20461 acgcgtctga gaagctcaaa aaaaattcct ctgaggcaca ggcgaagatc gacaagttaa
    20521 atcaaaccat caatgagaaa aaccaagaaa tcgaacagct gaagcaacaa gatggcacaa
    20581 aagcaaaaga agaagtaact gaagcacaaa gtaagacggc agaagagttc ggtaaattag
    20641 ccattgataa atcattaagc agaaatgaat taacggaaaa actaaaagat gtcgcaaccc
    20701 aagaagtgat tgacaaatta agtcctgcag acgatgatca ccaacatgat gattacggaa
    20761 gttactcctt tacattaggt gacgtgcaaa cttacgctca aacaagcagt gtaaaagagg
    20821 aacaaaattt tgtcgtattt gttgattata caatcggcaa tcctcaattc aaagatgtca
    20881 aaccacaaaa aataaaaggt ggattaactg tcactgaaaa acaggtagat ggtcaatgga
    20941 aagtcactga ctttagttat ttcactcgtt aggaggaaga aaatgagtaa gaaaaaagga
    21001 gaaagacaac gtttcaaaaa acgaaaaagt tatcttagat ggctgggaat ctttatgatt
    21061 ctcggatttt tgattcttgc tagtccgatt tacacgcatc agaaggtaga cataccagat
    21121 gaagtaagca cgaacgcatt tttaaacacg aaggaactaa cgatggtttc aaaagaaaaa
    21181 ggagcgaacc agcaattagt ttttgaattt gcaatcgacg atgaagatca aagcttatta
    21241 aaagaactgg ctaatcttaa ttatcgcgta gaagtaaaga cagctaaagg ggattatcaa
    21301 gcaataaaag ccaaagtgat taaggtgtct gatgattatt ttgcagtgaa gctatcgcat
    21361 gtaccagaag aatatattgc tatgcgatta accattattc ctgaaaaaat tgatccaaag
    21421 gtggatatgc aagaaccaca agatctgatt tattacgttc acgaagataa ggttaaagat
    21481 caagtgaaag atgaagatta tgaacaacat gcgatcaatt acaaagtgaa aggatacaag
    21541 aaagaacaaa aagcagctca aaaacaaatg gaatctttcc aagcaaccat tgatttgaat
    21601 gaagaattag tgaagaagct aaaagatcaa ttgccttatc aagtcgcaga tgatcaggaa
    21661 aatactgaaa acaagattaa tagctaccag caagaaattg aaacatcgaa aacgcaaatg
    21721 aaagatcaag aagagatcat tcaaaaactt caagaaaaaa ttgatcgtat gacaaaagaa
    21781 aagatttaga aggacaggtg gagtgaaaaa atgaagcgaa caaacttggc tgacaaagat
    21841 actttcgctg agcagaaagt attgattaaa gcctatctca aagttgtaaa agctaaagaa
    21901 atatgtcgag aagtgaattt atctaataca acaatatctg agttgaagaa tggtcgaaag
    21961 aatttagata ctttaaaatt cagatcattt gaagctctat atgtagctgc taaaaaatat
    22021 tttacagctg ataacaaata aaaaacagga gtattttatg tgaaaaaagt atataaaaaa
    22081 tgcttatgaa tcagctatat caccttaacc atttgttaat gagatgggag aaaacattaa
    22141 ccacgattct aacgcaaaca tcaagcacat aaaacattta acactcctcc tttctggctg
    22201 aagatttcaa ccaaacagga agacagcgta tcaattatag catacacttt ttcttaaatt
    22261 catttttcat atgacaaaat atttccttct aatcgacaaa gttagaagga ttttttattg
    22321 tctaaataca caaataacct taatttataa agagcataag gaaacgtggg aacacttttt
    22381 aaaaaaagtg ttccaatagg ctgtgcctat tacgtttctc aaaaataatt gtgggaacaa
    22441 aatatttaaa aataggatag tgaaaggata gcaaaggata gtaaaaggat agaagcagga
    22501 tagcgaaaag gataggctcc ttttcgctat cctttttaaa ttggaggaat taatatggaa
    22561 gtaagaataa gaaatgtaga tgagaaaatc gtaagagaaa ttgatcgaag atgtgacgaa
    22621 ctaagtaacc gaacgggtaa taaatggagt cgtaacgatt atttaaagct actcgttgaa
    22681 aatgattttg atcgtccttt gatggaatat aaacaagata aatttgatca attattaaat
    22741 cagttaatag ataaattttc ttttgttcaa agtcataata caaaagtatt agaagaatat
    22801 atttgtcaga ataatcaaat tatcgaactt ttaattgaac aaagtagagg aggagaagtt
    22861 taacatggcc aaagttatag acaagcatat ttatttttat gaaacacata tagatgttct
    22921 agatgaaatt attgcaactc atgcagaggt taaaaactat agtgaagccg ttcggtttct
    22981 atgtatgaat tatgattcag aagtggagaa aaaagtaaat tccaatcaag caaagttgaa
    23041 tgcaatggga aaagaaattt ctattttaac cgaaatggtt tcagagtttg cggatctcca
    23101 tttgaaagat acagggattt taattggtac aaattcaact gtttaccaag aagcaaaaga
    23161 gcgtgtggaa ggcaagataa aagcaaatca aacgaagaaa tattctccta agataaagaa
    23221 aacaataagc gaatcagtga ctacaaaaag tgaagtattt gatccaacaa ggttcttaaa
    23281 acgataaaaa ttagaaggag aatgtgatga gtaaatcacc tgcagtcatt ctcacttgtc
    23341 aatttacctt gcccaataaa aaatcttttt caacatacat tgattatatg acaagagaaa
    23401 aagcgctgga agaaaaggaa cgacgtacgc ccgaggaaga acaggaactg cgtctaatca
    23461 agaatgcttt agaggatttt tatattcctg aaggagaaac tttttcggag agtaagacgg
    23521 gtaaagaatt cagcgaaacg tcgaatgaag cgcaatcgtt gatgaaacat ggaatcaatt
    23581 ttgacaagtt agaagaacaa gattttacca aatatctttc ttatatgaca cgtcactatg
    23641 cgttagaaca aaagaaagta ctgacgcata gtgaacaaaa agaataccaa cgcttgaacc
    23701 aagcaataaa aaaatatcaa acagagccac acaaaaaagc ttcaggaaat cttcctggag
    23761 ttttttctat ggcttcagac gaagtgaaag gaagtgatct gaaggagatc aagcaaacct
    23821 tccaaagagg acaacaaaaa ggctcaatca tttatcaaga tgttgtctct catgatcatt
    23881 cgtatcttga aaaaatagga ttgtacgatc ccaaaacaga tactttagat gaagaaggtt
    23941 taaaagcagc tggaagaaaa atgatggaga cattgttcga gaaagagcaa ctgaatgaaa
    24001 caggctactg gatggcaacg attcaccgaa ataccaaaca tatccatatt cattttgcag
    24061 ttgttgaaaa aaaaaatacg cgtaaaccat acactgagat tgaaaatggt gtgagttata
    24121 tcgtaccgaa agggaagaga aggcaagcaa cattagacga aatgaagact aattatgtac
    24181 atgagttaga acgtttttct tatgaaaaaa atcgaatggg tatggagaaa cgaaatttat
    24241 tgacacggaa aagtgatttg cgtaacacct taaccaaagt agtgaaagag ccagtacgtt
    24301 atgatcgaca cgcaatgagc ttattaactg aagtatataa aaacttacct aaaaagcgtt
    24361 cggaatggca ttacggggat gaaaatcgaa ccaaactttc accatacaca agagagaaac
    24421 tcgatgagtt aacccaatat gtgttaacaa aagaacctgg atatcaagaa tatatacaga
    24481 tttcaaaagc attgaaagaa gaagcaaaaa gtctttatgg ggaaagcaag agagaatcaa
    24541 aggatgcaga aaaaaatgct ctctttgatt taaaaaagcg cacaggaaat gccattctaa
    24601 cagaactgaa gaaacaggat agtcaaataa aacctttaat agaaaggatg gatcagatgc
    24661 aatctattta caatgaacga tcctttcgaa cacttaaaca gttgccaacg aatgaataca
    24721 aatatagtta cgaagaattc atgaagcgca ggaaacaatt ccaacaaaga gttgtcacgc
    24781 aacggagttt gcgtcaatta aatcgaacga ttaatgccca tgaagaaaaa taccacgcaa
    24841 gtaaagcata cgaagagcaa caacgaagaa ttcaaatgga acaagaaaga cagtaggagg
    24901 aatagcaatg aaacaatatt tagatcaatg gaaagttata gaaggatcat taagagaaga
    24961 acgaatcgaa caacttcccg attgtttaga aaaagagcat ttgtttcaga taagagaaat
    25021 gttgagaaac gaacagtttg atccaaatca atttttagtc gttgagtact cagctactgg
    25081 tgtgtactgt tgtaatcatg tgaagggtga aaaatatttt atcattcaag aatatgaggg
    25141 caagttagcg ccttattata ctacttggga aatgaatgaa gaggggatca ataacttccc
    25201 gtgtaaaagc attgaagagt ctatttcact cacagaatgt tagttgttaa gtgaataaaa
    25261 gaagtaaaag agtcctgaac gtaattgtcc aggactcttt ttttcaatga attgttttcc
    25321 gccaattcaa tgagtggtgc gatttgcgac tacattatat caaaagaaag agaagggagg
    25381 aagaagaaat ggcttattca acgattggag ttgttgttga aaaaagcaga gacaatctcg
    25441 tttttgtgac agaaattcaa acgggcagag cttttgtcgt cacagacaaa gcggcaaaag
    25501 cgtatcaaag tggcgatatt ttaacgttaa atctgactac gaagacgttc gtcgatgcgg
    25561 cagaagatta tccatt
//
LOCUS       DASHHB010000034        25496 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.035, whole genome shotgun sequence.
ACCESSION   DASHHB010000034 DASHHB010000000
VERSION     DASHHB010000034.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 25496)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25496)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25496
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.035"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            127..3654
                     /locus_tag="U2K71_001667"
     CDS             127..3654
                     /locus_tag="U2K71_001667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PD-(D/E)XK nuclease family protein"
                     /protein_id="HEM7315749.1"
                     /translation="MSLQFILGNGKKDHRQVLIDEASAWLDQDASNQVFFLVPNYSKF
                     EQEQEILAEMRRRSGKKTFSTTNVQVFSFYRLAWYLLQQTTLLTGNELSESGSAMILR
                     QILEKNEEELTIFRGEINKRGFIRQLQELYSELRTGNIQPEDLMLLFGDSPNAEDQQL
                     KMKDLKLIFSAYDSELTQRALTNEDALSILANYMQTQDFNNVKFIVSGFSRINGQEYQ
                     LLQAMMEKGQLVVDLLLDRPYTADLPEVLTLFHETGKLYFRLFQSARERKVPILVDRF
                     APEQTRPTELQLLQTMWEETSSQKKAAVHKIGGQEHLHLWEAENPTEEVRQLAVEIRR
                     LVSEENYRYRDIQVLTKNMSLYGNILRPIFQEMAIPFYIDEEQMMENHPLIEWINSLF
                     ALDRYSYRLSDIMRFFKTELFLSTEAETELDEWKQERDEFRKKLDITENVALAYNYQG
                     TYWTREKDWQFVAYDFEAEQIEDVQYLEEQSNTIRRLFQKAVPVFLKKIKKSKTGQEA
                     ANVFYQFLIQSGVQQQLLFWRNQEVDRGNLEAARNHEQTWGALIDLLDEYVVIYGESA
                     FDWSLFQEIITSGLENLSYGKIPTAIDQVRINRLELVRPNQAAVTFALGLNDQVFPDR
                     KETKGLLSSEEREVLNQALPDGQFLFDPSKESISFEPFQAYLVFLSGTDRLYLSYAQS
                     YDTDGSLKMSPYLRRITEYLSVPVERKEHLTIESDPNCYVGTYRSGINTVNRIYRLAL
                     DEKKPVPKHWQKLKELVLNSSWRNFALQIFESQEHLNVPVSMPEKMAEELYGKEIYTS
                     ISRMENFYNCEYKYFMQFGLKLKERTVYGLTPAATGDFYHESLDRFFKLLFSNQLSLV
                     NMSDAQRRDFTEKVLQEVFGELRFEILDSSARMNYIRYQLGQTIQKVTWALQKQSKNT
                     GMHPLQTEILFGQIAGAKGITGLELPLSNGGKLHVRGKIDRIDVASEQEDTWLSVVDY
                     KSSGRSFDVTEAYYGMAMQLLTYLDVALMDAVRLTGKAAVKPAGSYYLHVHNPVLTET
                     DDVEKNTLKKFSYDGVFVDDPALLEVLDHSLEAKEQSLIYPVKKNAKDEYQKGSFSKD
                     KFFTEEELQLFMAHNRENMRLAGDAITSGEIKLNPAYKDKQRIACEFCPFRSICTFDV
                     MLKENNYHRLEKIDKEEALRRILKRSEEE"
     gene            3651..7373
                     /gene="addA"
                     /locus_tag="U2K71_001668"
     CDS             3651..7373
                     /gene="addA"
                     /locus_tag="U2K71_001668"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-exonuclease AddAB subunit AddA"
                     /protein_id="HEM7315750.1"
                     /translation="MNQIPLKPQNERFTDDQWQAIFDQGDNLLVSASAGSGKTTVLVR
                     RVIEKLKMGFDIDELLIVTFTEAAAREMKERIQEALQESVNSESDPVRRQHFTKQLVL
                     LPTANISTLHAFCLTVIRRYYYLIDIDPVFRMLTDETETILMKEDVWDELREALYAEN
                     DERFFQLTMNFSNDRSDDGLTNLVFSLYEFARANPDPQKWLEQLSDNYRLPEGLAKSR
                     LYQKQIRPLVLADIYQCVQLYEQMTQLAQGEGLEKMHEQVAGEQQQIKNIYEAFSQDR
                     LEEAYAGLEQLTFSTFKSSRKAELKEISNEVKGMRDKAKKLIQQISKSYFPVSPSQME
                     ELTDKALPLVEEMTKVTQSFMDGFSMRKREKGVLDFNDLEHLALQILTEKTKDVWLPS
                     EASKHYRKKFKEVMVDEYQDVNQLQEAILYWLREPDDTKGNMFMVGDVKQSIYSFRLA
                     DPSLFIGKYENFSKKEGGRRIVLAENFRSRKEVLSFTNLIFEQLMDPAVGQINYDEAA
                     KLIQGFSDFPENEQFEPEIMIYEKEQEESEIEIPTDDILEDKTEGELFMTGLKIRQLI
                     DSSFMIYDKKSKKSRPIEYKDIVLLTPTKKNNLTILEIFKTLDIPLEMNDAQNYFQAT
                     EIRTMISLLQLIDNPYQDIPLAAVLRSPIVGLIEPELASIRLANRAHTYYDAVLAYQA
                     SNEDELAAKLEHFGKQLEHWRELARRSSITDLLWDIYYETGYLEYVVGLPAGAQRQAN
                     LYALVDRAKAYEQSSFRGLYQFVRFIEKMQEKDKDLAEPVISIEDNAVRVMTIHASKG
                     LEFPVVFLLDMTKEFNLQDLRNRYAFEEKLGAGIRYMDPETRVLYDTLPFQAIKLAKQ
                     NKLLSEEMRKLYVGLTRAEQKLFIVGSYKNKEQMIQTWSEAADHEELVFDPALRLKGR
                     SSLMNWIGYGLIRHPEMQKYLEEEISTSLLQHSNAQFSISWMNQQSIIEQRQLLAEKE
                     LVNLDQQMKEDETLLADSLQKRLAYEYPYQASSQTTSYQSVSEIKRLFEDPDDTQESR
                     LTLESSQNKAASRQFRYTQEQLAEPKFLQKDRQVSAAAVGTATHALLQLLPLEMPTTE
                     SIHQKLQELVRKRLVDEKVAKKVDVSSIIWFFQTELGQQLIANKENVKREQPFSMLLP
                     ADEVFQDYPNQEDELLIHGIVDGYLEEKDHLNIYDFKTDFILHPDDPAEIDAIVQKYQ
                     GQLRLYQQAMSEALNKPVENVFLILLRVKQIININK"
     gene            7442..7783
                     /locus_tag="U2K71_001669"
     CDS             7442..7783
                     /locus_tag="U2K71_001669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315751.1"
                     /translation="MELTQTEFVLCPKFEKTFSILGKKWNGLIIDVLLENGPQRFVEL
                     AAKIPMVSDRVLVERLKELEKEGIVTRNFACSEKSRSEYLLTEKGADLQKAMRQIQVW
                     SEKWITEEECG"
     gene            8092..9135
                     /gene="pheS"
                     /locus_tag="U2K71_001670"
     CDS             8092..9135
                     /gene="pheS"
                     /locus_tag="U2K71_001670"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HEM7315752.1"
                     /translation="MSLQAQLESLKEATLVKITAAQDLNELNQIRVETLGKKGPITEV
                     LRGMKDLSPEERPKVGSFANEIRDLLTEEIERKKQVLEEEALNAALAKETIDVTLPGR
                     KVKHGTRHIVTQIMEEIEDIFVGMGYQIVEGYEVESDHYNFERMNLPKDHPARDMQDT
                     FYISDEILIRTHTSPVQARTMEKHDFSKGALRMISPGKVFRRDTDDATHSHQFHQIEG
                     LVVDKNITMGDLKGTLEVVMKKMFGEDREIRLRPSYFPFTEPSVEVDVSCFKCGGAGC
                     NVCKYTGWIEILGAGMVHPNVLKMSGIDPEEYSGFAFGLGPDRVAMLRYGVNDIRNFY
                     QNDLRFLSQFKGE"
     gene            9140..11560
                     /locus_tag="U2K71_001671"
     CDS             9140..11560
                     /locus_tag="U2K71_001671"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HEM7315753.1"
                     /translation="MLVSYKWLNRYVDLSNVTPKELADKMSVTGIEVEGVTVPEEGLK
                     KIVVGEVKECVPHPDSDHLSICQVDIGEDELSQIVCGAPNVKVGIKVIVALPNSRIAG
                     NVKIKKGKMRGQVSNGMICSLQEIGYSDSVIPKEYAEGIYYMPQEAVNGDPVFPYLDM
                     DDSIIELSITPNRADALSMRGVAYEVGAIYRQTPTFDDEKLVESSRPASDKIQVEVED
                     KEAVPAYQIRIIEGVRIQPSPQWLQNLLMNEGIRPINNVVDVTNYILLLFGQPLHAFD
                     YDKLKSKEIFVRRAKENETLVTLDGESRELTTDNLVITNGKEPVALAGVMGGLDSEIT
                     NETTTVALESALFDPISIRRTAKQFNLRSESSARFEKGINHATVGEASEVAAAMIVSL
                     AGGEVLQGAVKGSEIKAEDVSVKISLERINQYLGTSLVVKEVNEIFEALGFSYEEKEY
                     VYSVVVPPRRWDIQIEADIIEEVARIYGYDQLPSTLPSGETVAGSLSKEQETTRKIRT
                     IFEGSGLSEAISYALTTEEKSRQFVINESNITRLDWPMSEERSVLRMNLISGLLDDIS
                     YNTARKNTEIALYEIGRVFFQENDPRTHLPQEVKHAAIAISGIWEEKDWQTKAQNVDF
                     FTIKGLLENLFEQLGITEDVHYQTVTEMKEMHPGRTAAIYLGEKFIGFVGQVHPTITK
                     SYDIPETYVAEFDLSAIVEAAQKGIVFEAVTKFPAVSRDIALLVKETVTNQELTEVIR
                     SAAGRFLTDIQLFDVYQGENIEKGHKSMAYSLTFVNPEATLTDEEINKAMEKVEKALV
                     ENLEASIR"
     gene            complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="U2K71_001672"
     CDS             complement(11730..12740)
                     /gene="trpS"
                     /locus_tag="U2K71_001672"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HEM7315754.1"
                     /translation="MKNIILTGDRPTGKLHLGHYVGSLKTRVAMQADEDNQLFVMIAD
                     MQALTDNAKNPEKVSSNVLQVALDYLAVGLDPAKSTLFIQSQIPELAELTMYYLNLVS
                     VGRVRRNPTVKTEIEQKKFGESVPTGFFIYPVSQAADITAFKANLVPVGEDQKPMLEQ
                     TQEIVQSFNHTYGEVLVEPKGVFPPKGMGRLPGIDGNGKMSKSLGNGIYISDPADVLQ
                     KKVMSMYTDPNHIHVQDPGQVEGNMVFTYLDVFGTDKEAIEEMKAHYRRGGLGDVKIK
                     RYLIDVLEAEFAPIRARREELEKDPSAVMEILRKGSEEAAKVAAQTLSEVKSAMGINY
                     FH"
     gene            12994..13731
                     /locus_tag="U2K71_001673"
     CDS             12994..13731
                     /locus_tag="U2K71_001673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM7315755.1"
                     /translation="MAMIKFDHVDKYYGKFHALKNINLEFEKGEVVVVIGPSGSGKST
                     MLRCINGLETISSGKLLINDTDLHDKKTKLTEVRKNIGMVFQHFNLYPNKTVLENIKL
                     APIKVLKQDQATAVKNAEKFLKTVNMLDKKDSYPSMLSGGQQQRVAIARGLAMNPEML
                     LFDEPTSALDPEMIGDVLDVMKKLAHDGMSMIVVTHEMGFAKEVADRVIFMADGEVLE
                     DSRNVRDFFENPNEVRAQQFISKVINH"
     gene            13745..14560
                     /locus_tag="U2K71_001674"
     CDS             13745..14560
                     /locus_tag="U2K71_001674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HEM7315756.1"
                     /translation="MRRLKKIRLIFLLGIAFLALAGCKGNGLANEDILERSKSTNEII
                     WGVKYDTRLFGMMDIESRTVQGFDVDIAKAITKKILGDNGKTEFVEVTSKTRIPLLKN
                     GNIDAIIATMTITDERKKQVDFSDVYFDAGQALLVKKGSQIKSVDDLNASTTVLAVKG
                     STSAANIRQHAPDAKILELENYAEAFTALQSGQGDAMTTDNAILLGIADENPEYELVG
                     GTFTNEPYGIAINKGQENFLKAVNQALEEMHADGTYDKIYQKWFPNETEGKVE"
     gene            14573..15220
                     /locus_tag="U2K71_001675"
     CDS             14573..15220
                     /locus_tag="U2K71_001675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM7315757.1"
                     /translation="MSELIQTYGPIFVDGFKITLFSSVLALVFSLIIGTLMAIFQLSK
                     NKVVSALAKAYVEFFRNIPLLIIVMFFYVVVPIYWFNLDGFQAGTIGLTIYTSAFIAE
                     TVRSGIQTVPKGQTEAGLSSGFTYSEIMRYIVLPQAFKIVIPPLGNQFINLVKNSSIL
                     AIVAGLDLMYQGDLIASETFNTFDTYIIVGLFYLVITLPLSYLMVYLEKKWAVRS"
     gene            15236..15892
                     /locus_tag="U2K71_001676"
     CDS             15236..15892
                     /locus_tag="U2K71_001676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631187.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HEM7315758.1"
                     /translation="MDFSGAFSWMNIRFLLEGLKVTVEVSLFSIIFSFLIGGLTGVIR
                     FANIPFFSKILGVVIDIIRNLPLLLIIFFTYFALPQIGIQMNIFWSAVAALTIFESAM
                     LSEIFRAGLNAVPKGQMEAGLSTGLTYVESMRTIVLPQAFKSMIPAIVSQLISLIKDT
                     SLAVIISLPELSHQARIIYGQNTNYVLPMFIIMTLMYFVVCYALSLLSGYLEKRRYSY
                     "
     gene            16054..16875
                     /gene="racE"
                     /locus_tag="U2K71_001677"
     CDS             16054..16875
                     /gene="racE"
                     /locus_tag="U2K71_001677"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HEM7315759.1"
                     /translation="MIRLTDNRPIGFIDSGVGGLTVVKEALKQLPNENILFVGDTARC
                     PYGPRPAEQVIQYTWEMTDYLVEQGIKMLVIACNTATAVALEEIKAALSIPVIGVILP
                     GTRAAVKKTQNKQVGIIGTIGTVKSQAYEKALKEKVPELTVTSLACPKFVSVVESNEY
                     HSSVAKKIVAETLAPLTTKKIDTLILGCTHYPLLRPIIQNVMGENVQLIDSGAETVGE
                     VSMLLDYFNLSNSPQNGRTLCQFYTTGSAKLFEEIAEDWLGIGHLNVEHIELGGK"
     gene            16878..18233
                     /gene="rph"
                     /locus_tag="U2K71_001678"
     CDS             16878..18233
                     /gene="rph"
                     /locus_tag="U2K71_001678"
                     /EC_number="2.7.7.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HEM7315760.1"
                     /translation="MRHDGRKAQELRKLELQTNVFKYPEGSVVIRFGDTTVICSATIE
                     NSVPPFLRETGTGWVTAEYSMLPRATSTRNRRESSKGKLSGRTMEIQRLIGRSLRAVV
                     DLEKLGERSIIVDCDVIQADGGTRTASITGAFVALKLAIEKLLREKELSEDPIKEHLA
                     AVSVGILPDGTCVTDLDYQEDSAALVDMNLVMTESGKFVEIQGTGEEATFDGEQLNEM
                     LFFGKNAIEDLIKEQKHALLTEFAQNDERIEETKTIIIATRNPGKAEEFRNMFKEAGY
                     HVKTLLDYPELPDVEETGSTFEENARLKAETIAQLLDQPVLADDSGLKVDALGGMPGI
                     YSARFAGEQKSDAGNNAKLLYELTDVPDEKRTAQFHCTLVFAAPKKDSLVVEAEWPGR
                     VARIPSGENGFGYDPLFIPEGKEQTAAELSSEEKNKISHRAQAMKKLSAEWKQWLEGE
                     R"
     gene            18234..18752
                     /locus_tag="U2K71_001679"
     CDS             18234..18752
                     /locus_tag="U2K71_001679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HEM7315761.1"
                     /translation="MRYLVVSDNHGDRDILVDLVDRYRSEVDIFFHCGDSELEASDPL
                     WDTFQVVQGNCDYGPGFEQKKVIQTGQDTVFMTHGHLSNVRFGLTQLAIEARAATADM
                     VFFGHTHQIGCEMDQKILFLNPGSISQPRGPIQIPSYALIESLDDQISVQYYNRAHQA
                     VEELAFSFSRER"
     gene            18859..19359
                     /locus_tag="U2K71_001680"
     CDS             18859..19359
                     /locus_tag="U2K71_001680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM7315762.1"
                     /translation="MIGPIVREMLLENQETFLIPAENVANVMYHHPLSHGLLVLSKVG
                     YSKIPVLGKDDHFVGLVSLSDVVNKMIDLQTISMDPLEGLTVADVTETDVPTIDENWE
                     LEEVLHLLVDSPFLPVVTEDRIFKGIITRKELLKAVNFMVHELERRNILSPKIEELKE
                     QIDIVS"
     gene            complement(19639..20559)
                     /locus_tag="U2K71_001681"
     CDS             complement(19639..20559)
                     /locus_tag="U2K71_001681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HEM7315763.1"
                     /translation="MFSLLATAGTTDSSGETLTDQAVSKVNAFQRFWDGIDWDAILAA
                     FIQKSLYLIFLIVLFWILHRVGKYLIDKSFNQYSKKAILNESRLKTLHSLLNTIFHYT
                     LGFFFIYAILSAIGVPIGSLLAGAGIAGVAIGLGAQGFMSDVITGFFIIMEQQMDVGD
                     YIKLANLTIEGTVVSVGIRTLQLKATDGTVHFIPNRNITTISNLSRANMQVLLDIRIV
                     PEEGYDKIYEIIDRVNRTLAEKYQEELQTEPSIFGLVDLGNSNFAIRTVCYALNGKQY
                     ALKEEFLSSYVKELTASGFTIPNSPIAVGK"
     gene            20724..21737
                     /locus_tag="U2K71_001682"
     CDS             20724..21737
                     /locus_tag="U2K71_001682"
                     /EC_number="2.6.1.42"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid aminotransferase"
                     /protein_id="HEM7315764.1"
                     /translation="MQIDWNNLGFSYIKTPWRFIAKWKDGQWEEGELTEDNYLTIHEG
                     SPALHYGQQCFEGLKAYRRKDGKVNLFRPEQNSRRMNQSARRLLMPMVPEKMFVDAVK
                     QVVKANEDFVPPYGTGATLYLRPLLIGVGENIGVHPAPEYLFVVFCTPVGAYFKGGLK
                     PTNFIVSDYDRAAPKGTGAAKVGGNYAASLLPGTEAKELDYSDCVYLDPATHTKIEEV
                     GAANFFGITKDGKFITPQSPSILPSITKYSLLQLAEERLGLQAIEGDIFIDDLDQFAE
                     AGACGTAAVISPIGGIYHNNNLHVFYSETEVGPVTKRLYDELTGIQFGDIEAPEGWIE
                     VVE"
     gene            complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="U2K71_001683"
     CDS             complement(21847..22602)
                     /gene="fabI"
                     /locus_tag="U2K71_001683"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HEM7315765.1"
                     /translation="MSFLEGKKIVIMGVANKKSIAWGCAEAMLKQGAEIIYTYQNERM
                     KKSLVKLVGEEAFTVECDVSSDESIEKAMNAIGEYAGEIHGLVHAVAYANKEELSGNV
                     SDISRDGYQLAQDISSYSLIAVSKYAQPFLAPNSGIVSMTYLGSERAIPNYNMMGIAK
                     ASLEAAIRYLAAEFSPKGIRVNGISAGAIKTLAVTGVKDYQKLIQLSESRTPDQQGVT
                     IEEVGNACAFLISPLSSGIIGDIIYVDKGVHLS"
     gene            22822..23262
                     /gene="fabZ"
                     /locus_tag="U2K71_001684"
     CDS             22822..23262
                     /gene="fabZ"
                     /locus_tag="U2K71_001684"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HEM7315766.1"
                     /translation="MSYVLSATEVMELIPNRYPICYIDYVDTLEPGKKIVATKNVTIN
                     EDFFQGHFPDNPVMPGALILETLAQAGSILILKSEEFLGKRAYIGGINKAKFRQKVVP
                     GDVLKCHFEITKMKGSVGTALSAAYVDDKKVCECEFTFIVNQNA"
     gene            complement(23377..24138)
                     /locus_tag="U2K71_001685"
     CDS             complement(23377..24138)
                     /locus_tag="U2K71_001685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM7315767.1"
                     /translation="MKKNKVILSISLAAALLFAAGCSANNTTSSSSSTESSKSTSTAE
                     STTASSVDLNALELPQLTKDVAENEDLVEMVTNKGTIKIKLFPEHAPKAVENFMTHAK
                     DGYYNGLTFHRVIKDFMIQGGDPKGDGTGGESIWGEGFETEINNHLYNIRGALSMARS
                     QDPNSNGSQFFIVQNTDDMHDGLLKDDYPQAIIDAYKNGGYPSLDGHYTVFGQVIEGM
                     DVVDAIANIETDSSDKPKEEVKIDKINILQEAKQK"
     gene            24327..25421
                     /locus_tag="U2K71_001686"
     CDS             24327..25421
                     /locus_tag="U2K71_001686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEM7315768.1"
                     /translation="MGKLGISIYPERSTFEKDKAYLDLAHKYGYKRVFTSLLQINDDK
                     EKVLSEFKEVVDYANSLGMEVMVDINPALFEQLEISYDDLSFFHKMGAYGVRLDIGFT
                     GAEEAKMTRNPFGIKIEINMSSGTNYVDNIMSYSPNTDNLLGSHNFYPHRYSGLGYEH
                     FVFCSEKFRKYNLNTMAFVNSQSAEFGPWPTQDGLCTLEDHRDLEIATQVKHLILTGL
                     IDDISIGNAYASEEELKEMAEAFNADYPTLKVDTEEGITENERICLFDNLHSYRGDRS
                     EYILRSTMTRVYYKDKDFPPHNTRDMHHGDVLIDNEGYGQYKGETQIALKDMKNDGRV
                     NVVGRISDDELFLLDFLKPWSSFKLIENKK"
BASE COUNT         8446 a         4424 c         5385 g         7241 t
ORIGIN      
        1 cgtatactta aaaaagtaga aatatcagaa ccttaaaagg atataatttg aagaatatag
       61 aacaaatgtt cagtattttt tgatataata aaggagcacc gtatcatgtg ttgaaggagg
      121 aaaagcatgt ctttacaatt tattcttgga aacgggaaaa aagatcaccg tcaagtcttg
      181 atcgacgaag catccgcttg gctcgaccag gatgcaagta accaagtatt tttccttgtt
      241 cccaactaca gcaaattcga acaggaacaa gaaattttag cggagatgcg aagaagaagc
      301 ggaaaaaaaa ctttcagcac aacaaacgtt caagtattta gtttttatcg tctggcttgg
      361 tatttgctgc aacagacaac tcttcttaca ggaaatgaat tatcagaatc aggatctgca
      421 atgatcttgc gacagatttt agaaaaaaat gaggaagaat taacaatctt tcgcggagaa
      481 atcaataaaa gagggtttat tcgtcaatta caagaactct acagtgaatt gcgtacagga
      541 aatattcaac cagaagacct gatgctttta tttggtgatt cacctaatgc agaagaccag
      601 caacttaaaa tgaaagactt gaagctgatt ttttcagctt atgacagcga attgactcaa
      661 cgtgcactaa caaatgaaga tgcactatct atactagcaa attatatgca gacacaagat
      721 ttcaacaacg tcaaatttat tgtcagtggt ttttctcgaa tcaatggtca agagtatcaa
      781 ttactgcagg caatgatgga aaaggggcaa ttggttgtcg atttattgct ggatcgtcct
      841 tatacagcgg atcttccaga agtacttacg ctctttcatg agactggaaa gctttatttc
      901 cgtctttttc aaagtgcaag agaacggaaa gtccctatat tagtggaccg atttgcacca
      961 gagcagactc gtccgacaga gcttcaactg ctgcaaacaa tgtgggaaga gacatctagc
     1021 caaaagaaag cggcagtaca caaaataggg ggacaagagc atctccattt gtgggaagct
     1081 gaaaatccaa ctgaagaagt ccgacaatta gctgttgaaa tcagacgact agtcagtgaa
     1141 gaaaactacc gttatcgtga tatccaagtg ctgacaaaaa atatgtcttt atatggaaat
     1201 atccttcggc cgatctttca agaaatggca attccttttt atatcgatga agaacaaatg
     1261 atggaaaatc atccgctgat cgaatggatc aacagtctgt tcgctcttga ccgctattct
     1321 tatcggctaa gtgatattat gcgttttttc aaaactgaat tatttctgtc gacggaagca
     1381 gaaaccgaac ttgatgaatg gaaacaagag cgagatgaat tcaggaaaaa actggacatc
     1441 actgaaaatg tagcacttgc ttacaattat caaggaactt attggactcg tgaaaaagac
     1501 tggcagtttg tggcgtatga ttttgaagca gaacagattg aagacgtaca gtatttggaa
     1561 gaacagtcaa atacgattag acgattattt cagaaagctg taccagtatt tttgaaaaaa
     1621 ataaagaaaa gcaaaacagg acaagaggcc gcaaatgttt tttatcaatt tttgatccaa
     1681 agtggcgtac agcaacaatt attgttttgg cgtaatcagg aagtagacag aggaaactta
     1741 gaagcggcaa gaaaccatga gcagacatgg ggagcgctga ttgatctgtt ggatgaatat
     1801 gtagtaattt atggtgaatc ggcttttgac tggtcgctgt tccaagaaat cattacaagt
     1861 ggtctagaaa atctaagtta tggtaaaatc cctaccgcaa tcgaccaagt gcgaatcaat
     1921 cgtttggaat tagttcgacc aaatcaagct gccgtcactt ttgcattagg gttgaatgat
     1981 caagtgtttc cagaccgaaa agagacaaaa ggattgttgt ccagtgaaga acgagaagta
     2041 ttaaatcagg ccttgccaga tggacagttt ttattcgacc catcaaagga aagtatctct
     2101 tttgagcctt tccaagccta tcttgttttt ctttccggaa cagatcgatt atatcttagt
     2161 tatgcacaaa gctatgacac agatggttcg ctgaagatgt ctccttattt gcgtagaatc
     2221 acagaatatt tatctgttcc cgtcgagaga aaagagcatt tgacgatcga aagcgaccct
     2281 aattgttatg tagggactta cagaagcgga atcaatacag tcaataggat ttatcgatta
     2341 gctttagatg aaaaaaaacc tgtaccaaag cactggcaaa agctcaaaga attagtactg
     2401 aattcgtcat ggcgaaattt tgctttacag atttttgaaa gtcaggaaca tttaaatgtt
     2461 cctgtttcga tgccagaaaa aatggcggaa gagctttatg gaaaagagat ttatacttca
     2521 atttcacgta tggagaattt ttataattgt gaatacaaat acttcatgca atttggtttg
     2581 aaattgaaag aacgaactgt ttatggtcta actccagcag caactggtga tttttatcat
     2641 gaatcattgg atcgtttctt caagctactg ttttcaaacc aattatcctt agtgaatatg
     2701 tccgatgcgc agcgtagaga ctttactgaa aaagtgctgc aggaagtatt cggagaactt
     2761 cggtttgaga tattggatag ttctgcaaga atgaactata tccgctacca gttaggacag
     2821 acgattcaaa aagtgacatg ggctttgcaa aaacagagca aaaatacagg catgcatcct
     2881 cttcagacag agattctatt tggtcagatt gctggagcaa aaggtatcac aggtctggaa
     2941 ttaccgttga gtaatggtgg aaaactccat gttcgaggga aaatcgatcg aatcgatgta
     3001 gcttctgaac aagaagatac ttggctaagc gtcgttgatt acaaatctag cggacgttca
     3061 ttcgatgtca cagaagctta ttatggcatg gctatgcaat tattgacgta tctggacgta
     3121 gcattgatgg atgcggtacg tttgactgga aaggcggctg ttaaaccagc aggatcatat
     3181 tatttacatg ttcataatcc tgtattgacg gagacagatg atgtagagaa gaatacattg
     3241 aaaaaattta gttatgatgg cgtttttgtc gatgatcccg ctcttttaga agtactagat
     3301 cattcactgg aagcaaaaga acaatcactg atctatccag tgaaaaaaaa tgcgaaagat
     3361 gaataccaaa aaggtagttt cagtaaagac aaatttttca cggaagaaga actgcaacta
     3421 ttcatggcgc ataatcggga aaatatgcgg ttagctggag atgcgattac ttccggagaa
     3481 atcaaactaa atccagctta taaagataaa cagcggattg cctgcgaatt ttgtccattc
     3541 agaagcatct gtacgtttga tgtgatgctg aaagaaaata attaccaccg tctggagaaa
     3601 atagataagg aagaagcact gcgtagaata ttgaaaagga gtgaagaaga gtgaatcaaa
     3661 ttcctttaaa accgcaaaat gaacgattca cagatgatca gtggcaagcg atttttgatc
     3721 aaggagataa tctattagtc tctgcgtctg ccggatcagg taaaactaca gtgcttgttc
     3781 gaagagtgat tgaaaaacta aaaatgggtt ttgatatcga tgaattattg attgtgacat
     3841 ttactgaagc tgccgcaaga gaaatgaaag aaagaattca agaggcatta caagaatccg
     3901 tcaatagcga aagtgaccct gtacgtagac agcattttac taagcagtta gttttattgc
     3961 ccacagcgaa tatctcaacc ttacatgctt tttgcctgac agttattcga cgttattatt
     4021 acttaattga tattgatcca gtctttcgca tgttaacaga tgaaacagaa acgatcttga
     4081 tgaaggagga tgtgtgggac gagctgagag aagcacttta tgcagaaaat gatgaacgat
     4141 ttttccaatt aacaatgaat ttttctaatg atcgttcaga tgacggcttg acgaatctgg
     4201 tgttttctct ttatgagttt gcccgtgcca accctgaccc ccaaaaatgg ctagaacagt
     4261 taagcgataa ttatcgtttg cctgaggggc tagcgaaatc tcgattatat caaaaacaaa
     4321 tccgtccttt agttttggca gatatatacc aatgtgtaca gttatatgaa cagatgactc
     4381 agttagcaca aggtgaagga ttagaaaaaa tgcatgagca agtagctgga gaacaacagc
     4441 agatcaagaa tatctatgaa gcgttctcgc aagatcggtt agaagaagct tatgctggtt
     4501 tggaacaact aactttttca acttttaaga gcagtcggaa agcagaattg aaagaaattt
     4561 ccaatgaagt caagggcatg agggataaag caaaaaaact gattcagcag ataagtaaaa
     4621 gttattttcc tgtttcccca agccaaatgg aagaattaac ggacaaagca ctaccgttag
     4681 tcgaagaaat gacaaaagtc acgcaaagct tcatggacgg tttcagtatg cgtaaacggg
     4741 aaaaaggtgt gctggatttc aatgatctgg aacatttagc gttgcagatt ctcacagaaa
     4801 aaacgaaaga tgtatggctg ccaagtgaag cttcaaaaca ttatcgaaaa aaattcaaag
     4861 aagtaatggt agatgaatat caagacgtga accaattaca agaagccatt ttatactggc
     4921 taagagaacc cgatgataca aaaggaaata tgttcatggt tggggatgtc aaacaatcca
     4981 tctattcttt ccgtttagca gatcctagtt tatttatcgg gaaatacgag aatttctcaa
     5041 agaaagaagg cggacgtcga atcgttttgg ctgagaactt ccgctctaga aaagaagtcc
     5101 tttcttttac gaatcttatt tttgaacaac taatggatcc agcagtgggt caaatcaatt
     5161 atgacgaagc agccaaactg atccaaggat tttcagactt tccagagaat gaacagtttg
     5221 agccggaaat catgatctat gaaaaagaac aggaagaatc agagattgag attccgacgg
     5281 atgatatact ggaagacaaa acagaaggcg aactttttat gacaggacta aaaattcgtc
     5341 aattaattga ttcctccttt atgatttatg ataaaaaatc aaaaaaaagt cgtccgatag
     5401 agtacaaaga cattgttctc ttgacaccta caaagaagaa caacttaacg attttagaga
     5461 ttttcaaaac attggatatt ccattagaga tgaatgatgc tcaaaactat tttcaagcga
     5521 cagaaattcg cacgatgatc tcgctgctac aattaatcga taatccctat caagatattc
     5581 cgcttgcggc tgtattgcgc tcaccgatcg tgggtctgat tgaaccagaa ttagccagca
     5641 ttcgattagc taaccgtgca catacttact atgatgctgt gcttgcttat caagcatcaa
     5701 acgaagatga attagcagct aagctggaac attttggcaa gcaactagag cattggcgag
     5761 aacttgctcg ccgttcttcg atcactgact tgttatggga tatctattat gagacgggat
     5821 atctagagta tgtggtcggt ctgccagcag gggcacagag acaagccaat ctttatgcat
     5881 tggttgatcg tgccaaagct tacgagcaaa gttctttcag aggattatac cagttcgtgc
     5941 gtttcattga aaaaatgcaa gaaaaagata aagacttagc agaaccagtc atttcaatag
     6001 aagataatgc tgttcgcgtt atgacgatcc atgcaagtaa aggcttggaa ttcccagttg
     6061 tatttttgtt agatatgaca aaggagttca acttacaaga cttgcgcaat cggtatgctt
     6121 ttgaagaaaa acttggagca ggaattcgct atatggatcc tgaaacacga gtgttatatg
     6181 atacgttacc gtttcaagcc atcaaattgg caaagcaaaa caaattgttg tcggaagaaa
     6241 tgcgtaaatt gtatgtagga ttgactcggg cagaacaaaa gcttttcatt gtcggttcct
     6301 ataaaaataa agagcaaatg atccaaacgt ggtctgaagc agctgatcat gaggagttag
     6361 tttttgatcc tgcgctacgt ttgaagggca gaagcagctt gatgaattgg attggttacg
     6421 gattgatccg tcatcctgaa atgcagaaat acttagagga agaaatttcg acttctcttc
     6481 ttcaacattc aaatgcacag ttttctattt catggatgaa tcagcaatcg atcattgaac
     6541 aaagacagtt gcttgctgaa aaagaactgg ttaacttaga tcagcagatg aaagaagatg
     6601 aaactttatt agcagattcg ttacaaaaac gtctggcata tgaatatcct tatcaagctt
     6661 cttcacaaac cacaagttat caatctgtat ctgaaatcaa acgattattc gaagatccag
     6721 atgatacaca agaatcacgt ttgactctgg aaagcagtca gaacaaagca gcatcccgtc
     6781 aatttcgtta tacgcaagaa caactagcag aaccaaaatt tttgcaaaaa gatcggcaag
     6841 tgtcagcagc tgctgttgga actgctacgc atgctttgtt acaattactg ccattagaga
     6901 tgccaactac agagagtatc catcaaaaat tacaggaatt ggtaaggaaa cggttagtag
     6961 acgaaaaagt cgcgaaaaaa gtagatgttt cttctattat atggttcttt caaacagaac
     7021 ttggtcaaca gctgattgcc aacaaagaaa acgtaaaacg agaacaacct ttttccatgc
     7081 tgcttcctgc tgacgaagta tttcaagact atccaaatca agaagatgaa ctattaatcc
     7141 acggaatcgt cgatggatac ctagaagaaa aagatcatct caatatctat gattttaaaa
     7201 cagattttat tttacatccg gatgatccag cagaaataga tgcaatcgtc caaaaatatc
     7261 aaggacagct aagactttac caacaagcaa tgtcagaagc tttgaataag ccagtagaaa
     7321 atgttttcct aattttgctt cgagtgaaac aaattataaa cattaacaaa taattataat
     7381 tgagcaaact tgacttatgt tttgtaagga ttatgctata ctctgggaag aggtgagtat
     7441 gatggagcta acacagacag aattcgtttt atgccctaaa tttgaaaaaa cattctctat
     7501 tttaggaaaa aaatggaacg gattgattat cgatgtacta ctcgaaaacg gtccgcaacg
     7561 ctttgttgag cttgcggcaa aaatcccgat ggtcagtgat cgagtattag tcgaacgctt
     7621 gaaggaatta gaaaaagaag gaatagtcac tcgaaacttt gcctgtagcg aaaagagccg
     7681 ttcagaatat ctcttgactg aaaaaggagc agacttgcaa aaagcgatgc gtcaaataca
     7741 agtttggagc gaaaaatgga tcacagagga agaatgcggt tgattttgta aaaaaaatct
     7801 tgtaaactat agtccatacg aataagtaaa gaacgatgac gaaaaagagt agtttggcta
     7861 aaccagttca gggagatctg tcgcagactg aaagcagatc ctgtgtttga aaaatgaagt
     7921 tcactttccg agttcgtctg ataaaatcag accggcaatt gtcgttaacg aagtaagagt
     7981 gcggtgtaag tcatttatcc gcaaattagg atggtaacgc gaagacctcg ttccttaaca
     8041 ggaatgaggt ctttttgttt tgtatttgga atttaaagga ggaacaagca catgtctcta
     8101 caagcacaat tagagagctt gaaagaagca acgctcgtca aaatcacagc ggcacaagat
     8161 ttaaatgaat taaatcaaat acgtgttgaa acgttaggaa aaaaaggacc gatcacagaa
     8221 gtattacgtg ggatgaaaga tctaagtcca gaagagcgac caaaagtcgg aagtttcgca
     8281 aatgagatcc gagatctttt gacagaagag atcgagcgaa aaaaacaagt attagaagaa
     8341 gaagcgttaa atgcagcttt ggcaaaagaa acaatagacg tgacactacc tggtagaaaa
     8401 gtaaaacatg gtacacgtca tatcgtgaca caaatcatgg aagaaattga agatattttt
     8461 gtgggtatgg gctatcagat cgtagaagga tatgaagtag aatctgatca ttacaatttc
     8521 gaacggatga atctgccaaa agatcatcca gcaagggaca tgcaagatac tttctatatt
     8581 tcagacgaga tattgattcg gacacatact tcaccagtcc aagcacggac aatggaaaaa
     8641 catgatttct ctaagggtgc tctacgaatg atctcgccag ggaaagtctt ccgcagagat
     8701 acagatgatg cgacccacag tcatcagttc catcaaattg aagggctagt tgttgataaa
     8761 aacatcacaa tgggcgacct taaaggaaca ttagaagtcg taatgaaaaa aatgtttggg
     8821 gaagatcgtg aaattcgttt gcgtccaagt tatttcccat ttacggaacc atcagtagaa
     8881 gtagatgtca gctgtttcaa atgtggtggt gccggttgta acgtatgtaa atacactgga
     8941 tggatcgaaa ttttaggagc tggcatggta cacccgaatg tgttgaagat gtcaggaatc
     9001 gatccagaag aatattcagg ctttgctttc ggccttggac cagaccgcgt agctatgttg
     9061 cggtatggag tgaatgatat ccgtaatttc tatcaaaatg acttacgttt cttaagtcag
     9121 ttcaaggggg agtaagaaaa tgttagtttc atataaatgg ctcaatcgtt atgtagattt
     9181 atcgaatgta acaccaaaag aattagcgga taagatgtct gtgacaggaa tcgaagtaga
     9241 gggagtaacg gttccagaag aaggcttgaa aaaaatcgtt gtcggtgaag tcaaggaatg
     9301 cgtaccacat ccagattctg atcacctttc tatttgtcaa gtcgatattg gcgaagacga
     9361 actgtctcaa atcgtttgtg gagcgcctaa tgtgaaggtt ggaatcaaag tgattgttgc
     9421 acttcctaac tcacgtatcg ctggtaatgt aaaaatcaaa aaaggaaaaa tgcgtggcca
     9481 agtatcaaat ggcatgattt gctcattaca agaaattggc tattcagata gtgttatacc
     9541 gaaagaatac gctgaaggta tctattatat gccacaagaa gctgtaaatg gcgatccagt
     9601 ttttccttat ttggatatgg atgattcgat tattgaatta tctatcacac cgaatcgtgc
     9661 ggatgcattg tcaatgcgtg gcgtagctta tgaagttggg gcaatttatc gtcagactcc
     9721 tacattcgat gacgaaaagc tggtagaatc ctcacgtcct gctagcgata aaattcaagt
     9781 ggaagttgaa gataaagaag cagttcctgc ttatcaaatc agaatcattg aaggagtaag
     9841 aatccagcca agtcctcaat ggctgcaaaa tcttttgatg aacgaaggca ttcgtccaat
     9901 caacaatgta gtagatgtga cgaactatat tttgctttta ttcggacaac cactacatgc
     9961 atttgactac gacaaattga aatcaaaaga gatctttgtc cgccgtgcaa aagaaaatga
    10021 aacattagta acactagatg gagaatctcg ggaattgaca actgacaatc tcgtcattac
    10081 taacggcaaa gaaccagttg ctttagctgg tgtcatggga gggttagact cagagatcac
    10141 gaatgaaaca acaacagttg cactagaatc tgctttattt gatccaattt cgattcgtcg
    10201 tacagcaaaa caatttaatt tgcgtagtga atcctctgca cgttttgaaa aaggaatcaa
    10261 ccatgcgact gttggagaag ccagtgaggt tgctgcagca atgatcgttt ctcttgcggg
    10321 tggggaagtt ctacaaggcg cggtcaaagg ctcagagatc aaagcagaag acgtatcagt
    10381 gaagatttca cttgaacgca tcaatcaata tttaggaaca tctcttgtcg ttaaagaagt
    10441 aaatgagatt tttgaagcac ttggatttag ctatgaagaa aaggagtatg tttattccgt
    10501 agtagtacca ccaagacgct gggatatcca aatcgaagca gatattatcg aagaagttgc
    10561 tcgtatttat ggatacgacc agttgccatc tacacttcca agtggagaaa cggttgctgg
    10621 aagcttgtca aaagaacaag aaactactcg taaaattcgt acaatctttg aaggaagcgg
    10681 attgagcgaa gcaatcagtt atgcattgac aacagaagaa aaatcaagac agtttgttat
    10741 aaatgaaagt aatattacac gtttggactg gccaatgagc gaagaacgtt ctgtattacg
    10801 aatgaatttg atcagcggac tattagatga tatcagttat aacactgccc gaaaaaatac
    10861 agagattgct ttatatgaaa ttggtcgggt gttcttccaa gaaaatgatc cacgtacaca
    10921 tctgccgcaa gaagtaaaac atgcagcaat cgctatttct ggtatttggg aagaaaaaga
    10981 ttggcagacg aaagctcaaa atgtagactt tttcaccatc aagggtcttt tagaaaactt
    11041 atttgaacaa ctaggcatca ctgaagatgt ccattatcaa acagtgacag aaatgaagga
    11101 aatgcatcct ggaagaactg ctgcgattta ccttggcgaa aaatttattg gttttgtcgg
    11161 tcaagtgcat ccaacgatta caaaaagtta tgatattcca gaaacatatg ttgctgagtt
    11221 tgatttatct gctatagtgg aagctgctca aaaagggatc gttttcgaag cagtgacaaa
    11281 attcccagca gtcagccggg acatcgcttt gcttgtaaaa gaaactgtga caaatcagga
    11341 attaacagaa gttatccgtt ctgctgccgg aagattttta acagatatcc aattatttga
    11401 tgtctatcaa ggagaaaaca ttgaaaaagg acataaatcc atggcgtata gcctgacatt
    11461 cgtcaaccca gaagcaacat tgacagatga agaaatcaat aaagcaatgg aaaaagtcga
    11521 aaaagcgttg gtagaaaacc tagaggcaag tattcgctaa gaaggaaaat atagtaagta
    11581 acgatcaagt agcttttgaa gaccatacat ttgaaaaaaa gatgtgacac aacttcgtca
    11641 catctctttt tttgatttta aaaaaggaat agcacagaga cgagataaaa aaacaggcct
    11701 ttacagcctg cttttttaga gtaaatgaat taatggaagt aattgattcc cattgctgat
    11761 tttacttcag aaagtgtttg tgcagctact ttagcagctt cttcactacc tttacgtaag
    11821 atttccataa cagctgaagg atctttttcc aattcttctc ggcgcgcacg gatcggtgca
    11881 aattcagctt ccaatacatc aatgaggtat cgtttgatct ttacatcacc aagtccgccg
    11941 cggcggtaat gagctttcat ttcttcaatt gcttctttat ctgttccgaa tacatccaag
    12001 taggtgaaga ccatattccc ttctacttgt ccaggatctt gtacatggat gtggtttgga
    12061 tcagtataca tactcatgac ttttttctgt aagacatcgg caggatcgga gatataaata
    12121 ccgtttccta acgatttact cattttgcca tttccatcga ttccaggtaa tctacccatt
    12181 ccttttggtg ggaatacgcc ttttggttcc accaagactt caccatatgt atgattgaag
    12241 ctttgtacga tttcctgtgt ctgctctagc attggttttt gatcctcacc aacaggtaca
    12301 agattcgctt tgaatgcagt gatatctgct gcttgagaaa caggatagat aaagaatcct
    12361 gtaggcacgc tttcaccgaa ttttttttgt tcgatttctg ttttgacagt cggattgcgc
    12421 cgaacgcgtc ctacgctgac taagttcaaa taatacatgg ttaattcagc taattccgga
    12481 atttgtgatt ggataaataa agtagatttt gcaggatcta atcccacggc taaatagtct
    12541 aaagcaactt gcaagacatt tgatgaaacc ttctcaggat ttttagcatt atctgtcagt
    12601 gcttgcatgt cagcaatcat gacaaacagt tggttgtctt catctgcctg catagccact
    12661 cgggttttta gagaaccgac gtaatgccct aaatgcagct tcccagttgg tcgatctccc
    12721 gttaaaataa tatttttcaa aaaagacacc tctttacgat ttgtaagtct gagcacttaa
    12781 tatcttaccg tttttcctga aaagaaacaa cagttcctaa ggaaaaaaca aggaaagagg
    12841 ataaaataac taatagatta gaaatggaag aaatatttat gggagaataa acgttctaag
    12901 aaactttaca aattactaaa ctatcagata taatgtattt tattatacat ctttaaaaga
    12961 aatcttacta aataataatg ggagtgtaat tctatggcaa tgattaaatt tgatcacgtg
    13021 gataaatatt atggcaagtt ccacgcatta aaaaatatca atctcgaatt cgagaaagga
    13081 gaagttgtcg ttgtcattgg tccttctggt tcaggaaaga gcacgatgct tcgctgtatc
    13141 aatggattag agacgatctc ttcaggtaag ctgctgatta atgatactga cttgcatgat
    13201 aaaaagacaa aactgacaga agtccgaaaa aacatcggca tggttttcca acatttcaat
    13261 ttgtacccaa ataaaactgt tctggaaaac atcaaattag caccaatcaa agtgttgaag
    13321 caagaccagg caactgcagt caaaaacgcg gagaaatttt tgaaaactgt caatatgtta
    13381 gataagaaag attcttatcc ttccatgtta tccgggggac aacagcaacg ggtggcaatc
    13441 gccagaggtt tagcgatgaa tcctgagatg ctgctgtttg atgaacctac ttctgctttg
    13501 gatccggaaa tgattggtga tgtattagac gttatgaaaa aattggctca tgacggtatg
    13561 tcaatgattg tcgtgacaca tgaaatgggc tttgccaaag aagtggcgga tcgtgttatc
    13621 ttcatggctg atggagaagt attggaagac agcagaaatg tgagagactt tttcgaaaat
    13681 ccaaatgaag tccgagcaca gcaatttatc agtaaagtta tcaatcattg ataggaggaa
    13741 aaagatgcgc aggttaaaaa aaatacgcct gattttcctt ttaggcattg cttttcttgc
    13801 actagccgga tgtaaaggaa atgggttggc aaatgaagat attttagagc gaagcaaatc
    13861 taccaatgaa atcatctggg gtgtcaagta tgatactcga ttatttggaa tgatggatat
    13921 tgagagcaga actgtacaag gatttgatgt agatattgca aaagcaatca ctaaaaagat
    13981 ccttggtgac aatggaaaaa cagaatttgt tgaagtcact tctaaaactc ggattccatt
    14041 attgaaaaac ggaaatatcg atgcaattat tgcaacgatg acgatcacag atgaacgaaa
    14101 aaaacaggtt gacttttcag atgtctattt cgatgctggc caagctttgt tggtaaagaa
    14161 aggaagccaa atcaaaagtg tagatgattt gaatgcttct acaacggttc tagcagtcaa
    14221 aggttctaca tcggctgcga atatccgaca gcatgcacca gatgcaaaaa ttttagaatt
    14281 agaaaactat gcagaagcat ttactgcttt acaatctggt cagggagacg cgatgactac
    14341 ggataatgcg attttgttag ggattgcaga tgaaaatcca gagtatgagt tagttggtgg
    14401 aacgtttact aatgagcctt acgggattgc gatcaacaaa gggcaagaaa acttcttgaa
    14461 agctgttaac caagcgctag aggaaatgca tgcagatggc acatacgata aaatctatca
    14521 aaaatggttt ccaaatgaaa ctgaaggaaa agtagagtaa gggggagagg caatgagcga
    14581 attgatacaa acttatggac caatctttgt cgacgggttc aagatcacat tgttttcgag
    14641 tgtcttagca cttgtgttta gtttgattat cggaacgtta atggcaatct tccaattatc
    14701 aaagaataaa gtagtgagcg ccttagcaaa ggcatatgta gaatttttta gaaacattcc
    14761 attattaatc atcgtgatgt ttttttatgt tgtcgtaccg atttattggt ttaatctaga
    14821 tggattccaa gcaggtacga ttggattaac gatctataca tctgctttta ttgctgaaac
    14881 agtacgttca gggattcaaa ccgtaccaaa aggtcaaaca gaagcaggac tatcttctgg
    14941 ttttacctat tcagaaatca tgcgttatat tgttttaccg caagcattca aaatcgttat
    15001 cccgccatta ggcaaccaat ttatcaacct cgtaaaaaac tcatctattt tagcaatcgt
    15061 agctggttta gatttgatgt atcaaggaga cttgatcgct agtgaaacat tcaatacctt
    15121 cgatacgtat atcatcgttg ggttatttta cttagttatc acgctaccgt tatcttactt
    15181 aatggtttat cttgagaaaa aatgggcggt tcgttcatag aaggagggga aatacatgga
    15241 ttttagcggc gctttttcat ggatgaatat ccgtttttta ttggaaggat taaaggttac
    15301 tgttgaagtt tcattgtttt cgatcatttt tagtttcttg atcggtggtc tgacaggtgt
    15361 gattcgtttt gcgaatatcc ctttcttttc caaaatcctt ggtgtagtga ttgatattat
    15421 acggaatctt ccattattat tgatcatttt cttcacttac tttgccttac ctcaaatcgg
    15481 cattcagatg aacatcttct ggtccgctgt cgcagcatta acgatttttg agtcagctat
    15541 gttgtcggaa attttccgag ctggactcaa tgctgttcca aaaggacaga tggaagcagg
    15601 attatcaacg ggactgactt atgtagaatc gatgcgaacg atcgtgttac cgcaagcatt
    15661 taaatcaatg atcccggcaa ttgtgagcca attgatttca ctgatcaaag atacttcatt
    15721 agcagtaatc atttctttac ctgaactttc tcatcaggca cgtattattt atggacagaa
    15781 caccaattat gtgcttccga tgttcatcat catgacactt atgtacttcg tggtatgtta
    15841 cgcgctttct ctgctttccg gttacttgga aaagagacgg tacagttatt aatagatgat
    15901 tcttttagcg gttgtggcaa actaaagtca caaccgcttt tcctctttcc attcgaaaaa
    15961 aagagatttc gattgtttta agcaagtaag agcacttgaa aattcacgct aaaaagtaca
    16021 caatagggag gatagaattt attgaaaaaa aggatgatac gattgacaga taatcgccct
    16081 atcggattta ttgattcagg tgtcggcggc ttgactgtag taaaagaagc cctgaaacaa
    16141 ttaccgaatg aaaatatttt atttgtagga gacacagcac gctgcccata tggccctaga
    16201 cccgcggaac aggtaatcca gtatacttgg gaaatgacgg attatctggt ggagcaagga
    16261 atcaagatgc tggtgatcgc ctgcaatacc gcaactgcgg tggctttaga agaaatcaaa
    16321 gctgctcttt ctattccagt catcggtgtg atccttcccg gtactagagc ggcagtaaaa
    16381 aaaacacaaa ataaacaagt tggcattatc ggtacgattg gtacggtaaa aagtcaagct
    16441 tatgaaaaag cactgaaaga gaaagtacca gaattgactg tgacaagtct tgcttgtccc
    16501 aaatttgttt cagttgtcga aagtaatgaa taccattcat cggtggcgaa aaaaattgtg
    16561 gcagaaacat tagctccttt aaccactaaa aaaatcgata cattgatttt gggatgcacc
    16621 cattatccat tattacgccc catcattcaa aatgtaatgg gagaaaatgt tcaactgatc
    16681 gattctggag cagaaacagt aggtgaagta tctatgctgt tagattattt caatttgagc
    16741 aattcaccgc aaaatggtcg gacattatgc cagttttata caactggctc tgccaaactt
    16801 ttcgaggaaa tagctgaaga ctggcttgga atcggacact taaatgtaga acatatcgaa
    16861 ttgggaggaa aataaggatg cgtcatgacg ggagaaaagc ccaagagctt cgcaaactgg
    16921 agcttcaaac aaatgttttc aaatatccag aaggatcggt agtgatccgt tttggtgata
    16981 caacagttat ttgttctgca acaatagaaa attctgttcc cccttttttg agagaaacag
    17041 gaacaggatg ggtgacagca gaatatagta tgctgcctcg agcaacgagt acgagaaatc
    17101 gtagagaaag cagcaaaggg aaactttctg gtagaacaat ggaaatccag cgtcttattg
    17161 gtcgttcctt gcgtgcagta gtagacttgg aaaaattagg agaacgtagt atcatcgtag
    17221 actgcgatgt gatccaagcg gatggcggta ctagaacagc aagtatcact ggggcatttg
    17281 tggcattgaa attagccatt gaaaaacttt taagagaaaa agaattgtca gaagatccaa
    17341 taaaagaaca tcttgctgca gttagcgtag gaatcttacc agatgggact tgcgtcacag
    17401 atttggatta ccaagaagat tccgcagcac ttgtagacat gaatcttgta atgacagaat
    17461 ctggaaaatt cgtggagatt caaggaactg gagaagaagc aacgtttgat ggtgaacagt
    17521 tgaatgaaat gctctttttt ggaaaaaacg cgattgaaga tttgatcaaa gagcaaaaac
    17581 atgctttgct aacagagttt gctcaaaatg atgagcggat agaagaaaca aaaacaatca
    17641 tcattgcgac aagaaatcca ggaaaagcag aagagtttcg aaatatgttc aaagaagcgg
    17701 gatatcatgt aaaaacatta cttgactatc ctgaattgcc agatgtggaa gaaactggca
    17761 gcacatttga agaaaatgcc cgattaaaag cagaaacgat tgcacaattg cttgatcagc
    17821 cagtcttagc agatgattca ggtttgaaag tggatgcttt aggcggtatg ccaggaatct
    17881 attctgcacg ttttgcaggg gaacaaaaaa gtgatgcggg gaataacgcc aaattattat
    17941 atgaattgac agatgtgccg gatgaaaaaa gaacagcaca attccattgt acgttagtat
    18001 tcgcggcacc caaaaaagac agccttgtag tagaagcgga atggcccggt cgagtagccc
    18061 gtataccatc tggcgaaaat ggctttgggt atgatccttt atttatccca gaaggtaagg
    18121 aacaaacagc agcagaatta tcgagcgaag aaaaaaacaa gatcagtcat cgggctcaag
    18181 caatgaaaaa attgagtgcc gagtggaaac aatggctgga aggagagaga tagatgcggt
    18241 atttagtagt aagtgataat catggagaca gagatatcct cgttgactta gtagatcgtt
    18301 atcgttctga agtggatatc tttttccatt gtggtgattc ggaattagaa gcttctgacc
    18361 cattatggga tacattccaa gtggttcaag gtaattgtga ttatggacca ggattcgaac
    18421 agaaaaaagt gatccaaact ggtcaggata cagtattcat gactcacggt catttatcga
    18481 atgtccgatt tggtttgaca cagttagcaa tcgaagctcg agctgcaact gccgatatgg
    18541 tgttttttgg tcatacccat caaataggct gtgagatgga tcagaaaatc ctgtttttga
    18601 atcctggtag catttctcag cctagaggcc cgatccaaat cccatcatat gctcttatcg
    18661 aaagtttaga tgaccaaatt tctgttcaat actataaccg tgcccatcag gcagttgaag
    18721 agttggcctt ttctttttcg agagagcgct gaataaaaaa actctaattt ttaataaaaa
    18781 taagctttat tttatgaatt ttgcatccgg tttcggtaaa atagtagtgt tgaatgaaat
    18841 agaaatggag gccatcagat gattggacct atagtaagag aaatgctgct ggaaaatcag
    18901 gaaacgtttt tgattcctgc agaaaatgtg gcaaatgtta tgtatcatca tccattgtcc
    18961 catggtttac ttgtgttatc gaaggtcggt tattccaaaa ttcctgtatt aggaaaagat
    19021 gatcactttg taggactagt aagtttatca gatgtggtca ataaaatgat tgacttgcaa
    19081 acaatcagca tggacccgtt agaaggacta acggttgctg atgtgacaga aacagatgtt
    19141 ccgacaattg atgaaaattg ggaactagaa gaagtactcc atttgttagt cgattcgcct
    19201 tttttaccgg ttgtcacaga agatcggatt ttcaaaggaa tcattacaag aaaagagctg
    19261 cttaaagccg taaacttcat ggtgcatgaa ttagaacgtc gtaatatatt gtctccgaaa
    19321 atcgaagaat taaaagaaca aatcgatatc gtgagctaaa gaacgagaaa ctaaaagata
    19381 atcaaaaaat aagtagaaaa aacagcgtac cagttttgac tttatcttga aaaagaggct
    19441 gggacaaaag cgtttaactc caagaaataa gaaggaattc acgaaaattg cttttcaaat
    19501 ttttgtgaat ttcagcttat ttccgaagga gttgcttctg cttccgccgt ttatacgtgt
    19561 ttagagcgtg ggacaaaagt gttctttact tttgtcccac gctcttttta gtgagattcg
    19621 ttgcatcgtt tgtccaaatt attttccgac agcaattgga ctgttcggaa tcgtgaaacc
    19681 agaagctgtc aattctttga cataggagct caagaactct tcttttaagg catattgttt
    19741 tccgttcaat gcgtagcaga ccgtacgaat cgcaaaattg ctgtttccaa gatccactaa
    19801 tccaaagata gaaggttcag tttggagctc ttcttggtat ttttcagcta atgtacgatt
    19861 tactcgatca atgatctcat agattttgtc atatccttct tcaggaacaa tccgaatatc
    19921 taaaagtact tgcatatttg cacgagagag attgctgatc gtggtgatgt ttcgattcgg
    19981 gatgaaatgg acagtaccat ccgttgcttt cagctgaagg gtacggatac caacagatac
    20041 gacggttcct tcaatcgtta agtttgctag cttgatgtag tccccaacat ccatctgttg
    20101 ttccatgata ataaaaaatc cggtaataac atcactcatg aacccttggg caccaagacc
    20161 gattgccaca cctgcaattc cggcaccagc caaaagtgat ccgatcggca caccgatagc
    20221 agataaaatt gcatagataa aaaagaaacc aagggtataa tgaaaaatag tatttaaaag
    20281 actatgtaaa gtctttaagc ggctctcgtt tagaatggct tttttagaat actgattaaa
    20341 agacttatca atcagatatt ttcccacacg gtgaagaatc caaaaaagga cgatcaaaaa
    20401 aatcagatat aaactttttt ggataaaggc agccaaaatg gcgtcccaat caatcccgtc
    20461 ccaaaaccgc tgaaacgcat ttactttgct gacagcttga tctgtcaatg tctcgccaga
    20521 agagtctgtc gtaccggcag ttgctaataa tgaaaacata aaatcctctt ttctaaaatt
    20581 tgattctttc tgtattgtac caagaagttt tcaaaaaagg aaatagcaaa aataactata
    20641 atgaaggaaa aaagctgaaa ataaaatgac aaataatatg gataatgata aaatgtggag
    20701 aaaataacga aggtggtaag tatatgcaaa tcgattggaa caatctaggt ttttcttata
    20761 tcaaaactcc ttggcgtttt attgccaaat ggaaagacgg acaatgggaa gaaggagaat
    20821 tgacagaaga taattacctg acgattcatg aaggctctcc agcgcttcat tatggtcagc
    20881 aatgttttga aggtctaaaa gcttatcgtc gaaaagacgg caaggtcaat ctctttcgac
    20941 ctgaacaaaa tagtcgacgg atgaatcaaa gtgctagacg attgctgatg ccaatggtcc
    21001 ctgaaaagat gttcgttgat gctgtcaagc aagtggtaaa agcaaatgaa gattttgtgc
    21061 cgccctatgg aacaggcgct acattatact tgcgcccttt actgattggg gtcggtgaaa
    21121 acattggtgt ccatccagct ccagaatatt tatttgtcgt cttctgtacg ccagtaggag
    21181 cgtatttcaa aggtggacta aaaccaacga attttattgt atctgattac gatcgtgctg
    21241 ctcctaaggg gactggtgca gcaaaagtcg gcggaaatta cgcagcgagt ttacttccag
    21301 gaacagaagc aaaagaactg gattactcag attgtgttta tcttgatcca gccacacata
    21361 cgaaaatcga agaagtaggt gcagcaaatt tctttggaat tacaaaagac ggaaaattca
    21421 tcactccgca atcgccctct atcttaccaa gtatcacgaa atattccttg ttgcagttag
    21481 ctgaagaacg actaggactg caagcaattg aaggagatat tttcattgat gatctagatc
    21541 aatttgcaga agctggtgca tgtggtacag cggctgttat ttctccaatc ggtgggattt
    21601 atcacaataa caatcttcat gtattttata gtgaaacgga agtaggacca gtaacaaaac
    21661 ggttgtatga tgaattgaca ggtattcaat ttggagacat agaagcacca gaaggctgga
    21721 ttgaagtcgt ggaataattt caaagtaatg agttgaaaaa aacgtaaaaa gaaaaaatca
    21781 ggaagagact ttaaaaagtt ttttcctgat ttttatatgt tttactttaa agaatgcaac
    21841 aaaagtttat gaaagatgga cacctttgtc aacgtagata atatcaccga tgattccgct
    21901 ggaaagtggg ctgattaaga aagcacaagc attaccgact tcttcgatcg tcacaccttg
    21961 ctgatcaggc gtacgagatt cagataactg gatcagtttt tgatagtctt taacacctgt
    22021 gacagctaat gttttgattg ccccagctga gattccattg acccgaattc ctttaggcga
    22081 gaactcagct gccaaataac ggatagcagc ttcaagagaa gctttcgcaa tacccatcat
    22141 gttataattc gggattgcgc gttctgaacc tagataagtc atgctaacga tcccgctgtt
    22201 tggtgcaaga aacggttgtg cgtattttga tacagcaatt aaagagtagc tgctgatatc
    22261 ttgagctaat tgatagccgt ctctggaaat atcagacaca tttccggaca attcttcttt
    22321 gttggcgtaa gcaactgcat ggacaaggcc gtggatctcc ccagcatatt caccgatggc
    22381 attcattgct ttttcgatgc tttcgtcgct agaaacatca cattctacag tgaatgcttc
    22441 ttcgccgacc aatttgacta atgatttttt cattcgttca ttttggtaag tatagataat
    22501 ttctgcccct tgtttcaaca ttgcttcagc acaaccccaa gcaatgcttt ttttgttagc
    22561 gacgcccata atgacgattt tttttccttc tagaaatgac ataatgactc tcctttgttt
    22621 ctgataaaaa tttgatagtc gtaaaaaaat agagaataat agctaaaata acttcttgat
    22681 ttcttaatta ttagtcaaaa tgttttgatt gtcaaactat tttttcttaa attaaaaaaa
    22741 gattgctaaa tatttaggaa tatgatactt tgatagtcaa agtaatgaaa atggcaattg
    22801 cctgaaagaa ggagtttaat tatgtcttac gtattatctg ctactgaagt aatggaattg
    22861 attcctaatc gttatcctat ttgttatatt gattatgtag atactttaga accaggaaaa
    22921 aagatcgttg cgacaaaaaa tgtaacaatc aatgaagatt ttttccaagg tcactttcct
    22981 gacaatccag tgatgccggg tgcgttgatt ctagaaacac tggcacaagc aggttcgata
    23041 cttatcctaa aatcagaaga gtttctagga aaaagagctt atatcggtgg gatcaataag
    23101 gccaaattcc gtcaaaaagt tgttccagga gatgtgctga aatgtcattt tgaaatcacc
    23161 aaaatgaagg gatcagtcgg tacagcgtta tctgcagcat atgtagatga taaaaaagtc
    23221 tgtgagtgcg agttcacgtt catcgtcaac caaaatgctt gactataaaa cctgatatag
    23281 caaaacaaaa ggaagcttac ttgcttttta tgtaaaaaga agacgcctga atcctagcat
    23341 acgttgtcat ctatgcagga ttcagacgtt atttttttat ttttgttttg cttcttgcag
    23401 tatattgatt ttatcgattt ttacttcttc ttttggtttg tctgaactat ctgtttcgat
    23461 atttgcaata gcatccacga catccattcc ttcaatcact tggccgaaga cagtataatg
    23521 gccatctagt gaaggataac cgccgttttt ataagcgtcg atgattgctt gaggatagtc
    23581 gtcttttaaa agaccatcat gcatatcatc tgtattttga acgataaaga actggctgcc
    23641 gttactgtta ggatcttgtg agcgagccat agataacgca cctcttatat tataaaggtg
    23701 gttgttgatc tctgtttcaa atccttctcc ccaaatgctt tcccctccag tgccatcgcc
    23761 tttaggatcg ccaccttgga tcatgaaatc tttgatgacg cgatggaaag tcagtccgtt
    23821 gtagtaaccg tcttttgcat gtgtcataaa gttttcaacc gcttttggtg cgtgttcggg
    23881 aaataatttg attttgattg tgcctttatt cgtcaccatt tcgaccagat cttcattttc
    23941 ggcgacatct ttcgtcaatt gtggtaattc caatgcgttc aaatcgacgc tactggcagt
    24001 cgtgctttcc gcagtagatg tactttttga actttctgta gacgaactag aagaagtcgt
    24061 gttgtttgca gaacaaccag cagcaaacag caatgctgct gctaaactaa tacttaaaat
    24121 aactttattt ttcttcataa ctgttccttt ccaatttact attttgttat ctttaatgat
    24181 aacaaaatag agggaaaata cctacttaaa ttattggaat ttatcattta ttttaaaaga
    24241 aaaagaaaag gtgtagacca tttactttgt gtttgctata atgtaagtgg atacaaaaac
    24301 gaaaagatgc aggaggaaat ggtaatatgg gaaaattagg aatctcgatt tatccagaac
    24361 ggtcaacatt tgaaaaggac aaagcgtact tggatttagc tcataagtat gggtataaac
    24421 gggtttttac cagtttgctg cagatcaatg atgataaaga aaaagttctt tcagaattta
    24481 aagaagtagt tgattacgcg aatagcctag gcatggaagt aatggttgat atcaatccag
    24541 cgttatttga acagttggag atttcttacg atgacttatc ttttttccat aagatgggtg
    24601 cctacggggt ccgcttagat atcggattca caggagcaga agaagcaaaa atgacgcgca
    24661 atccttttgg tatcaaaatc gagatcaata tgagttcggg cacgaattac gttgacaaca
    24721 tcatgagtta ttcgccaaac acggataatt tattaggttc tcataatttt tatcctcatc
    24781 gatactctgg tttaggatac gaacactttg ttttttgttc agaaaaattc agaaaatata
    24841 atttgaatac gatggcattc gtcaattcgc aatctgctga gtttggccct tggcctacgc
    24901 aagatggctt gtgcacgctg gaagaccacc gagatcttga aattgcgaca caagtgaagc
    24961 atttaatcct tacaggattg attgatgata tttctattgg aaatgcgtac gcaagtgaag
    25021 aagaattgaa agaaatggca gaagcattta acgcggatta tccaacattg aaagtagata
    25081 cggaagaagg tattacagaa aacgaacgaa tctgcttgtt tgataacctt catagttatc
    25141 gaggggatcg ctcagaatat atccttcgtt caacgatgac tcgtgtatac tacaaagata
    25201 aagatttccc gcctcataat acgcgagata tgcatcatgg ggatgtactg attgacaatg
    25261 aagggtacgg tcaatacaaa ggagaaacac aaatcgcctt gaaggatatg aaaaacgacg
    25321 gacgtgtcaa tgttgtagga agaatctccg atgacgaatt attcctcctt gactttttga
    25381 aaccatggtc aagctttaaa ttaattgaaa ataaaaaata gataattagt tattgcagaa
    25441 ataatccgct agaaatagcc agttatttct gcttttttat tttgtttttt tatagg
//
LOCUS       DASHHB010000035        24501 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.036, whole genome shotgun sequence.
ACCESSION   DASHHB010000035 DASHHB010000000
VERSION     DASHHB010000035.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 24501)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 24501)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..24501
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.036"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(24..599)
                     /locus_tag="U2K71_001687"
     CDS             complement(24..599)
                     /locus_tag="U2K71_001687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300462.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM7315769.1"
                     /translation="MNKNKMATRVLTVMALCIGINYIGGTIALWLRLPIYLDSIGTIF
                     AGALLGPVPGMLTGLSSGILSGVTTDIFSLYYSPIQILTGLLAGLILPRYLGKDKNSL
                     KLLLLALILSVPGTIVSSIITVQLFGGITSSGSSMIVQLLHGLGLSQTTSVILVQAGT
                     DYLDRLLSIVVVSLVITMLPQRLIYSKRKIG"
     gene            complement(604..1578)
                     /locus_tag="U2K71_001688"
     CDS             complement(604..1578)
                     /locus_tag="U2K71_001688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="purine nucleosidase"
                     /protein_id="HEM7315770.1"
                     /translation="MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIG
                     TQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNFPMILRKQAEPL
                     HAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCS
                     PVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLK
                     AITRFYFDFHWKQERILGCVINDPLAVAYFIEEAICEGFKSFTAVETQGISRGQTLVD
                     RYEFWQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG"
     regulatory      complement(1602..1646)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00522"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="PreQ1 riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="preQ1"
                     /db_xref="RFAM:RF00522"
     gene            1861..2829
                     /locus_tag="U2K71_001689"
     CDS             1861..2829
                     /locus_tag="U2K71_001689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM7315771.1"
                     /translation="MVIFYFILILIIIGISYHFLSYFYAKKLVRAGVQRGYDWYEDTG
                     HRLMDPAAVTSVEKKRQKLEEECESFWEQGTPKTIKSYDGLKLAGQMFIHPNKQNKWV
                     ICVHDYRSTGKRDMSHIGKRYAEKGFNVLVPDLRAHGESEGEIIGMGWLDRLDLIAWI
                     QLILDEQPNASIILHGGSMGASTIMMASGEKLPSAVKGFILDSGYVSVYAEFRYMLSK
                     ITVFPKKMVMRYANHYAQKYAGYSLKQASATRQLGSNHLPLLVIHGERDHFVPTEAAY
                     TIQNATAGDKALLLVPEAEHLEASMKDPKTYWTVIFSFIEQRITMN"
     gene            2926..3219
                     /locus_tag="U2K71_001690"
     CDS             2926..3219
                     /locus_tag="U2K71_001690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HEM7315772.1"
                     /translation="MRSAAYGNQRRKNRLALFNDEQQEIGEMTWSDAGPDIMIVDHTF
                     VDPAYRGQKLAEKLVYTGVELARRDGKKIIPLCPYAKKEFEKKPEYQDVLRQS"
     gene            3337..3573
                     /gene="secG"
                     /locus_tag="U2K71_001691"
     CDS             3337..3573
                     /gene="secG"
                     /locus_tag="U2K71_001691"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HEM7315773.1"
                     /translation="MYNLILGIVIVLSIVMVIAIMMQPSKQNSAASAFTGGADQLFGK
                     QKARGFEAVMQRSTAVMGAIWMILLFVLAYLSSK"
     gene            3676..4431
                     /locus_tag="U2K71_001692"
     CDS             3676..4431
                     /locus_tag="U2K71_001692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEM7315774.1"
                     /translation="MRRVRQLPKPLYAKHGKRAVLLLHAYSGSPNDVRMLARFLEKAD
                     YTVYAPLFKGHGTMEPYDILQEKAESWWADTKKAIHFLQSEQFSDIAVLGLSMGGIFA
                     VRALEEESVIGGGFFCSPLSPVKTNVPENFEKYVRQVLKTAGKSEKEINEKAVAYRSL
                     AEQQLMDIQDQAAIVESRLSDIQQPIFLAQAGKDEMIDPNGVFETARKLSRQRVTLQW
                     YPESGHVITVGTARRELEKDVLEFLEKLPWNEE"
     gene            4434..6791
                     /gene="rnr"
                     /locus_tag="U2K71_001693"
     CDS             4434..6791
                     /gene="rnr"
                     /locus_tag="U2K71_001693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease R"
                     /protein_id="HEM7315775.1"
                     /translation="MTKETIKEKILFFMESSRKRSFSMEEIAEGLGFQKSDDFKLLVQ
                     TIAQMEREQSIVFNKKGKVKLPLKPILIEGTFRANERGFGFVTIDPEEDDIYIPKEAT
                     GFAMDGDTVAIDIMKRANTDMDRGAEGKVVEIRDRATTQLAGEFVAYSEEEIQETDLY
                     GVVIPKDRKLNQFKVYAAAEGVRPVDGSIVLIELTHYPEKGYATSLEGIIKQVIGHKN
                     DPGMDILSIVVANGIPTKFPDDVLSEADEVPDQISEKDITGRRDLRNQLIVTIDGEDA
                     KDLDDAVTVKKLDNGNYFLGVHIADVSYYVTQGSQLDREAYERGTSVYLTDRVIPMIP
                     QRLSNGICSLNPHVPRLTMSCEMEIDPNGQVVSHDIFPSVIQTTERMTYTAVNQILED
                     QDEETMQRYAQLVPMFQEMGELHRILETMRIKRGAISFEDREAKILVDAEGHPNDIQL
                     RSRGIGERLIESFMLAANETVAEHYNKLNLPFIYRIHEQPKEEKMQRFFDFASALGIL
                     VRGTKGTITPKDLQHVIEGVEDKPEAAVINTMLLRSMQQARYSEDNFGHYGLAADYYT
                     HFTSPIRRYPDLIVHRLIRSYSEDKSESNQSSWAAELPEIADHSSKMERRAVETEREV
                     DSMKKAEFMMDKVGEEFDGIISSVVKFGLFIELPNTVEGLIHINELKQDYFHFVENHL
                     ALVGERTGLTFKIGQKVRVKVVKADPDERAVDFELVSAEEVTALERPKTRNGKSGKRE
                     GKQAGKRPERNGKKNKRYSDSKQTFGKKKGKKPFYKGVKKKKKKK"
     gene            6813..7277
                     /gene="smpB"
                     /locus_tag="U2K71_001694"
     CDS             6813..7277
                     /gene="smpB"
                     /locus_tag="U2K71_001694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein SmpB"
                     /protein_id="HEM7315776.1"
                     /translation="MPKGEGKLIAQNKKARHDYSIIDTMEAGMVLQGTEIKSIRNSRI
                     NLKDGFIRVRNGEAFLHNVHISPYEQGNIFNHDPLRTRKLLLHKKQIIRLENELKNTG
                     ITVVPLKVYIRNGYAKVLIGLAKGKKSYDKREDLKRKDIDRQIDRTLKNFSR"
     gene            7579..8298
                     /gene="atpB"
                     /locus_tag="U2K71_001695"
     CDS             7579..8298
                     /gene="atpB"
                     /locus_tag="U2K71_001695"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HEM7315777.1"
                     /translation="MDERSLTFHIGPIWFDGTVCIMVLLTCLIVFFLAYFFTRNLKLK
                     PTGKQNAIEWVIDFSRGIVTDNLPRKELSNFHLLAFTLFLFVFVANNIGLVTKIVLPN
                     ETTLWKSPTADPFVTLTLAFIMILLTHLFGVKKLGFKGYFVNSFLKPYSFMFPMKLIE
                     EFTNLLTLALRLYGNIYAGEVLLTLIAKMMTSLGWFSLPLAIPLEMVWIAFSLFIGSI
                     QAFVFVTLSMVYMSHKIEVEE"
     gene            8351..8566
                     /gene="atpE"
                     /locus_tag="U2K71_001696"
     CDS             8351..8566
                     /gene="atpE"
                     /locus_tag="U2K71_001696"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HEM7315778.1"
                     /translation="MNYIAAAIAIMGAAIGAGYGNGQVISKTIESMARQPEMSGQFRT
                     TMFIGVALVEAVPILGVVIALILVFAV"
     gene            8657..9181
                     /gene="atpF"
                     /locus_tag="U2K71_001697"
     CDS             8657..9181
                     /gene="atpF"
                     /locus_tag="U2K71_001697"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HEM7315779.1"
                     /translation="MLNQLAIAEVGNAMLGNIIVVSGSFLILLALLKHFAWGPISDIL
                     KKREDKIANDLDSAEQSRINSAKMEQEREQQLLASRSDAADIIKNAKESGELSRQNIL
                     KDAQEEAARLKSKAQADITVERDSALNSVKDDVAELSLQIAEKILNKELSPEMHESLI
                     NQYIEGLGSSNETR"
     gene            9168..9710
                     /locus_tag="U2K71_001698"
     CDS             9168..9710
                     /locus_tag="U2K71_001698"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit delta"
                     /protein_id="HEM7315780.1"
                     /translation="MKLDKYTVGRRYGKALFELAIDSNQAEEIYQDLVSLRQIYEQVP
                     GLGNMLSDVRLEPHEKREIMDKLVSGYDGIVKNFLEVVYEYNRMDDLLFMIEEYERRY
                     DEHQGLLLGSVTTAVPLSDEQRIQLEKNVAKTMDYQSVELKQIVDSSIIGGAIVEANH
                     RVIDGSIRTQLEKMRNQLNR"
     gene            9733..11289
                     /locus_tag="U2K71_001699"
     CDS             9733..11289
                     /locus_tag="U2K71_001699"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HEM7315781.1"
                     /translation="MAIKAEEISALIKEQIENYQNVLSVEEIGTVTYVGDGIARAHGL
                     ENAMSGELLEFSNGSYGMAQNLESNDVGIIILGDFESIREGDKVKRTGKIMEVPVGEA
                     LIGRVVNPLGQPIDGLGEIVTDKARPVEAMAPGVMQRKSVNEPMQTGLKAIDALVPIG
                     RGQRELVIGDRKTGKTSIAIDTIINQKGQDMICIYVAIGQKDSTVRTQVETLKKYGAM
                     DYTIVVNAGASQPAPLLYIAPYAGTAMGEEFMYNGKHVLIIFDDLSKQAVAYRELSLL
                     LRRPPGREAYPGDVFYLHSRLLERAAKLSDDLGGGSMTALPFVETQAGDISAYIPTNV
                     ISITDGQIFLESDLFYAGTRPAVDAGLSVSRVGGSAQIKAMKKVAGTLRLDLASYREL
                     EAFTQFGSDLDAATQAKLNRGRRTVEILKQKLHAPLAVEKQVVILYALTHGFLDSIPV
                     DSILDFEHELFEYLDTNHSDIFETIRTTKDLPEEEALNSAIQEYKDMFLATKGSNSST
                     EDKLKSIQNA"
     gene            11303..12205
                     /locus_tag="U2K71_001700"
     CDS             11303..12205
                     /locus_tag="U2K71_001700"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit gamma"
                     /protein_id="HEM7315782.1"
                     /translation="MGASLNEIKTRIASTKKTSQITRAMQMVSASKLTKSEASSQKFQ
                     VYATKVREIVTHLTATQLNDIASENPRGDINYNSMLLSRPVKKTGYIVITADGGLVGG
                     YNSSILKQTMSILEEDHQSPDDYVMIAIGGTGADFFKARGINLAYELRNLSDQPSFDE
                     VRKIVNMATTMFQNEVFDELYVCYNHHINSLTSQFRVEKMLPISDLDPEEATTFEQEY
                     IFEPSKEEILSQLLPQYAESLIYGAIVDAKTAEHAAGMTAMKTATDNAANIIDELTVS
                     YNRARQGAITQEITEIVAGASALE"
     gene            12228..13634
                     /gene="atpD"
                     /locus_tag="U2K71_001701"
     CDS             12228..13634
                     /gene="atpD"
                     /locus_tag="U2K71_001701"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HEM7315783.1"
                     /translation="MSSGKIVQVIGPVVDVEFSLDQSLPDINNALVVYKNDENKSKVV
                     LEAALELGDGVIRTIAMESTDGLQRGMEVIDTGKAISVPVGKETLGRVFNVLGDTIDL
                     ETPFPEDAERSEIHKKAPAFDELSTSTEILETGIKVIDLLAPYLKGGKVGLFGGAGVG
                     KTVLIQELIHNIAQEHGGISVFTGVGERTREGNDLYYEMKDSGVIEKTAMVFGQMNEP
                     PGARMRVALTGLTIAEYFRDVEGQDVLLFIDNIFRFTQAGSEVSALLGRMPSAVGYQP
                     TLATEMGQLQERITSTKKGSITSIQAIYVPADDYTDPAPATAFAHLDATTNLERKLTE
                     QGIYPAVDPLASSSSALAPEIVGEEHYKVATEVQHVLQRYRELQDIIAILGMDELSDQ
                     EKVLVSRARRVQFFLSQNFNVAEQFTGLPGSYVPVEETVKGFREILEGKYDDLPEEAF
                     RSVGRIEDVVEKAKTLGY"
     gene            13648..14070
                     /locus_tag="U2K71_001702"
     CDS             13648..14070
                     /locus_tag="U2K71_001702"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit epsilon"
                     /protein_id="HEM7315784.1"
                     /translation="MDQYLTVNVVTPAGLVYDHHAAIVVARTTAGELGILPKHAPIIV
                     PLTIDEVRVKRTDSDTHVDWIAVNGGIMEVRDNVVSIVADSAERERDIDVSRAERAKQ
                     RAERQIEEAKAKQDTNELKRATVALHRAINRIKVSKHS"
     gene            14539..14769
                     /locus_tag="U2K71_001703"
     CDS             14539..14769
                     /locus_tag="U2K71_001703"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1146 domain-containing protein"
                     /protein_id="HEM7315785.1"
                     /translation="MQVYGIDAVLRIVSHLAFIYLAFWSLRSLRIETFFRPMHNTQIR
                     MAIVLFSIFLGYTASNFFLEVIALCRNLFVSL"
     gene            14866..16167
                     /gene="murA"
                     /locus_tag="U2K71_001704"
     CDS             14866..16167
                     /gene="murA"
                     /locus_tag="U2K71_001704"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HEM7315786.1"
                     /translation="MEEIIVRGGNQLNGTVRIEGAKNAVLPILAASLLAEEGITTLDN
                     VPILSDVFTMNQVIRHLNVDVDFDEQKNQVTIDASRQLEIEAPYEYVSQMRASIVVMG
                     PLLARNGHAKVAMPGGCAIGKRPIDLHLKGFQALGAKIIQKNGYIEAIADELIGNTIY
                     LDFPSVGATQNIMMAAVKAKGTTIIENVAREPEIVDLANILNKMGAQVYGAGTETMRI
                     EGVDHLHAVNHSIVQDRIEAGTFMVAAAMTQGNVLIADAISEHNRPLISKLIEMGAEI
                     IEEEGGVRVIGPKHILPTDVKTMPHPGFPTDMQAQMTAIQLVAEGTSVVTETVFENRF
                     QHLEEMRRMNAHVKIDGNVAIMDGNHELQGAEVYATDLRAAAALVLAGLKANGITRVR
                     NLNYLDRGYYNFHIKLQQLGADVERVDMDQTSAEKTAQTIA"
     gene            16180..16356
                     /locus_tag="U2K71_001705"
     CDS             16180..16356
                     /locus_tag="U2K71_001705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HEM7315787.1"
                     /translation="MSNQRYILLTLIKILVVILLLILLFVAGTMIGYGVIGGGNPFKV
                     FQPSLWIHIRDFFH"
     gene            16491..16787
                     /gene="yidD"
                     /locus_tag="U2K71_001706"
     CDS             16491..16787
                     /gene="yidD"
                     /locus_tag="U2K71_001706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HEM7315788.1"
                     /translation="MKKILIYLVRGYQRFISPAFPPSCRYYPTCSNYMIKAIQTHGAL
                     KGTVMGTARILRCHPFVKGGIDYVPLTFRLTRNKKDKKEPDYKKVVKNTGSTER"
     gene            16851..17318
                     /locus_tag="U2K71_001707"
     CDS             16851..17318
                     /locus_tag="U2K71_001707"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7315789.1"
                     /translation="MIEELNKKDLPWSLLLDADPDKEKVQVYVSRGSGWIWKEKEKTI
                     GVLIYVAREKEYEIVNVAVTPTYQGKGIGGKLLETAFQKLSQLASNQTRIIIRTGTTS
                     SAALHLYQKMGYVEIGRVKDYFIHHYSEPIFENGDQLRDQVILAIHLPKNSFN"
     gene            17403..18287
                     /locus_tag="U2K71_001708"
     CDS             17403..18287
                     /locus_tag="U2K71_001708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM7315790.1"
                     /translation="MGILVFDFGGSAVKYGCWDGKEIKGKGQFATPESWEEMKTQLFQ
                     VYKKILVSFEVEGIGISSPGVVNNEKQVIEGISAIPYIHGFNIFRDLETLFQLPVTIE
                     NDANCAGMAEFYEGAARDYQDVAFVVAGTGIGGALFTKGQINKGAHLYGGEFGLMFLD
                     GDKTFSKLGTAVQMAWRYCERKGLDKNAYTGKDVFELAETGDAIAKEEVESFYTYLTK
                     GLFSIQFSFDPEVIVLGGGVSAKEGLIDEIKSRMKKLTEQFDLHDFEPQILLCEYRND
                     ANLIGAAANYLAVRQGEN"
     gene            18401..18637
                     /locus_tag="U2K71_001709"
     CDS             18401..18637
                     /locus_tag="U2K71_001709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2969 domain-containing protein"
                     /protein_id="HEM7315791.1"
                     /translation="MMSKKNKDIEVRIEEVKKDIKGKNYEVTQLFIGKKMIGEILAYG
                     PKEYEIFFGEEDFGKEKSLENAIETVIRHWNLHE"
     gene            18725..18805
                     /locus_tag="U2K71_001710"
     tRNA            18725..18805
                     /locus_tag="U2K71_001710"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18759..18761,aa:Tyr,seq:gta)
     gene            18812..18883
                     /locus_tag="U2K71_001711"
     tRNA            18812..18883
                     /locus_tag="U2K71_001711"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:18844..18846,aa:Gln,seq:ttg)
     gene            19009..19179
                     /locus_tag="U2K71_001712"
     CDS             19009..19179
                     /locus_tag="U2K71_001712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315792.1"
                     /translation="MKWKEKVDEAAEKLYDLVKSEKYNIEVNIPKKGGKAVQVKSKRP
                     TNHTKKWMAKNR"
     gene            19399..20106
                     /locus_tag="U2K71_001713"
     CDS             19399..20106
                     /locus_tag="U2K71_001713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM7315793.1"
                     /translation="MSNQLPVYIQIHDQLKNEIEQGIWKVGDRLPSERELAVKFNVSR
                     MTLRQAIQNLADEGILERKIGSGTYVAREKVQEKMSGATSFTEIMESQNRVPSSRTIS
                     YFLTSPSSSEMEKLNLSKDDTILRMERIRFADDVPICFEVASVPQKLIDGYSKAEITE
                     SLYRTLEEKGGKKIGPANQTITAVLASEKIADYLEVKRRDAVLRLRQISYFEDGTPFE
                     YVRTQYVGNRFEFYLEK"
     gene            complement(20165..20809)
                     /locus_tag="U2K71_001714"
     CDS             complement(20165..20809)
                     /locus_tag="U2K71_001714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002400550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YigZ family protein"
                     /protein_id="HEM7315794.1"
                     /translation="MIERYRTIKENGQSEIEIKKSRFICSLRRIESEDQAKNFIQEIK
                     KEHWKANHNCSAFIIGEKNEIQRSSDDGEPSGTAGVPMLEVLKKNQLINVAAVVTRYF
                     GGTKLGTGGLIRAYTHAVSQALSDIGIVEGTLQQEISVMITYPQLGKLQNYLELQHFF
                     IKEIQYTDQVSVICAVDEKSANHFKESIIELLNGQVQFEEGSVSYHEQLITDHI"
     gene            20936..21223
                     /locus_tag="U2K71_001715"
     CDS             20936..21223
                     /locus_tag="U2K71_001715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315795.1"
                     /translation="MQERMKKYDAITHYLKNNGGSQVTLTFTQFDELLFPSNGLPKTA
                     RESTDWWANDYKHPEKGAYGWINAGYEVVVINLDKEYVVFNKLVKSSWLFD"
     gene            21331..21651
                     /locus_tag="U2K71_001716"
     CDS             21331..21651
                     /locus_tag="U2K71_001716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7315796.1"
                     /translation="MAKKTIMLVCSAGMSTSLLVTKMQKAAEAKGIDSDIFAVSASDA
                     DNNLANKDVDVLLLGPQVRFMKADFEKRLEPKGIPLDVINMADYGMMNGEKVLDQAIA
                     LIGE"
     gene            21969..23045
                     /locus_tag="U2K71_001717"
     CDS             21969..23045
                     /locus_tag="U2K71_001717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEM7315797.1"
                     /translation="MFDQPEAKPREVVFFWTGQLTEKQTEIAKRILCHSEKRHHLIWA
                     VTGAGKTEMLYPILVETLKAGGRVAICTPRVDVCNELFLRYRYVFPEEKITLLHGNTA
                     TAYTFTSFVICTIHQLYRFYRSFDLIVIDEIDAFPYNGDAGLAHAVQTAIRSNGRFIY
                     LSATPDEKLLKEIKQTFELHKLALRFHRRLLPEPVLVFMNNWRQKCGNKKKINSLVRI
                     IQQLIKENRILLFCPSITMMHRLKITLQEAFPEYEITDVSSQDDQRAKKVQKMREAIY
                     EILLTTMILERGVTFERISVIVLGADHPVFTKSSLVQIAGRADRKGPYTNSRVYFFYE
                     EKTSAIRKACQEIKEMNEEGKQML"
     gene            23042..23761
                     /locus_tag="U2K71_001718"
     CDS             23042..23761
                     /locus_tag="U2K71_001718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HEM7315798.1"
                     /translation="MRCSCCGKDIIQNLTFSEIIGVSKSRLPQTCSVCRAQFHLLEAK
                     GCAGCQKPIHLKYEETKKKANTSFVYCNDCQEWKKKYPAYDFQHRAFFTYDEKMQAWI
                     NRYKFMGDIRLAGTFAYKWTQIKKDYPAYIYCPIPLSEKRWLERGFNQVSEMLKAAKI
                     PFQMLLKREQHLLAQAKNTRKERLEMPQPFELAVPLSKIQHKKILIIDDVYTTGQTLF
                     HAADCLQNAQPKIIRTISLAR"
     gene            23889..24440
                     /gene="raiA"
                     /locus_tag="U2K71_001719"
     CDS             23889..24440
                     /gene="raiA"
                     /locus_tag="U2K71_001719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HEM7315799.1"
                     /translation="MFRYNVRGENIEVTEAIRDYVEKKVGKLERYFSDAPDATAHVNL
                     KVYTEKTAKVEVTIPLPYLVLRAEETSPDLYASIDLVVDKLERQIRKFKTKINRKSRE
                     TGMNTADAAALFNEEDENENDSELDIVRTKRLSLKPMDSEEAVLQMNMLGHNFFIFED
                     AETNGTSIVYRRKDGKYGLIETD"
BASE COUNT         8119 a         4276 c         5269 g         6837 t
ORIGIN      
        1 tttgctaatc cagctctgtt tattcagcct atttttcttt ttgaatatat caagcgttgt
       61 ggcaacatag tgatgactaa tgaaacaacg acgatagaca acaaacgatc aaggtagtct
      121 gtacctgcct gtaccaagat cacacttgtt gtctgactta aacctaaccc atgtaatagc
      181 tgaacgatca tactggatcc tgaggaggtg atcccgccaa ataactgaac ggtaatgatc
      241 gaagaaacga tcgttccagg taccgacaaa atcaaagcga gtaataacag tttcaagctg
      301 tttttatctt tgccgagata tcttggcaaa atcaatccag caagcaagcc tgtgagaatt
      361 tggattggcg aataatacag agaaaaaata tctgtagtca ctccacttaa aataccgctg
      421 gacaatcctg tcagcattcc aggtacaggc ccaagcaatg caccagcaaa aatcgttccg
      481 atagaatcga gatagattgg caaacgaagc cataaagcaa tcgttcctcc aatataattg
      541 atcccaatac acaaagccat cactgtaagt acgcgagttg ccattttgtt tttattcatt
      601 ttgttagccc atctttaacc tttccaaatc tttcataatc gtttcttctt gtgcatttaa
      661 aagaacggtt aaaaactttc ggaaaaaaag acgagtatct acttttgtca taattttact
      721 gttagcaggt ttctgccaaa attcataacg atcaaccaat gtttgtccac gacttattcc
      781 ttgagtttcc acagcagtga agcttttgaa accttcacag atggcctcct cgataaaata
      841 agcaacagct aacggatcat tgatgacaca tcctaaaatt ctttcttgtt tccagtggaa
      901 gtcaaaataa aaacgagtga tcgcttttag atactctccc tcttcaggat tcatttgaca
      961 gcagtattcg aggatagtcg gtgtcaagac gatttctctt gtcacatcta acccaaccat
     1021 ttctatcgtt tgtttgagat tctcgaatac ataagaagct gcttcaggat cacaccagta
     1081 attgtattca gcaactggcg aacagttacc atggcttttg taagttccac ccatcgagac
     1141 aaaacgttcc atatgtttcc caatatttgg gttgactttc aaagcagaag cgatattggt
     1201 caaaggaccc aaagcaatga ctgatgtatt tgtcttttct ttgaaataat cagccaaaaa
     1261 atcaacggca tggagaggtt ctgcctgttt tctcaagatc atcgggaaat ttgtttcccc
     1321 taaaccatcc ataccatgag tgtcttgtgc actgatgaaa ggttgcttca aaggtttttc
     1381 cattccttga taaacaggaa tgtctaaacg atcacagcgt tccaggcatt tcagcacatt
     1441 ctgcgtacct atatctgcag ggacattccc acaaactacg gtaatagcaa ctacttcgat
     1501 atcaggggat ttgacagcta atgataaagc taaagtgtcg tcgatccctg gatcgcaatc
     1561 aataattact tttcgcatta tttccaatct cctttcttta gagatactta gttttttatt
     1621 ctgggaagtt tccgaaccag tccaagacga gcgtcttgat tataatgcac gaaattagta
     1681 tactggcttt tattgcgaat gtaaatgctc tcttacaagt aaatctattg aggagaactt
     1741 taagtaaatg atacaatgaa aaagaaatgg aatttacgtg tgccgaaaat aaagaatcct
     1801 atggatcggt acattggata atagatagat tatagtttgg cagaaagcgg cgggtgtaca
     1861 atggttattt tttatttcat tcttattctg ataattatag ggatcagtta ccattttctt
     1921 tcttattttt atgcaaagaa attagtaaga gcgggagtac agagaggata tgactggtat
     1981 gaagacactg gtcatcgttt gatggaccct gctgcagtca cgtcagtaga aaaaaaacga
     2041 caaaaattag aagaagagtg tgaaagcttt tgggagcagg gaacacccaa aacaatcaaa
     2101 agttatgacg ggttgaaact agctggtcag atgttcatcc atccaaataa gcagaataaa
     2161 tgggtcattt gcgtacatga ttaccgaagc acagggaaaa gagacatgtc tcatataggt
     2221 aaaagatatg cagagaaagg attcaacgtt cttgtacctg atttacgtgc gcacggagaa
     2281 agtgaaggag aaatcatcgg aatgggatgg ctagatcggc tagatttaat tgcctggatc
     2341 caactcatct tagatgaaca gccgaatgcg tccattattt tacacggcgg atcaatggga
     2401 gcttctacga tcatgatggc aagtggtgaa aaacttccat cagcggtcaa aggatttatt
     2461 ttagacagcg gatatgtttc tgtttatgca gaattccgat atatgttaag taaaatcact
     2521 gtctttccta aaaaaatggt catgagatat gcgaatcatt atgcccagaa atacgccggt
     2581 tactccttaa agcaagcatc agctacgaga cagcttggca gcaatcatct gcctttatta
     2641 gtcatccatg gtgaaagaga tcattttgtc cctacagaag cagcgtacac gattcaaaat
     2701 gctacggcag gggataaagc actgctgctt gttccggaag cagaacactt ggaagcatcg
     2761 atgaaagatc cgaaaaccta ttggacagtg attttttcat ttattgaaca aagaataacg
     2821 atgaattaat tttctcttct tttttgtaag taattcgagg ataaataatt gtttatagat
     2881 caggagactg ctaaaataag gtaggaataa aaaaatatca gttatttgag gagtgcggca
     2941 tatggaaatc aaagaagaaa aaatcgtctt gctttattca atgatgaaca acaagagatc
     3001 ggagaaatga catggtcaga tgcgggccca gacatcatga tcgttgacca tacatttgta
     3061 gatccagcat atcgtggtca aaagttagct gaaaagctag tgtatactgg agtagaatta
     3121 gcaagacgag acgggaaaaa gatcattcct ttatgccctt atgcaaaaaa agagtttgaa
     3181 aagaaaccag aatatcaaga cgtcttacgt cagtcataag aataacagct gaaagttttg
     3241 aactttcagc tgttattcca tttatataga gttgtcacgg acttttatct atgataaagt
     3301 taaagagacg atatcttttg ggaaggaggc tgcgatatgt ataatttaat tttgggaatc
     3361 gtgatcgttt tatcgattgt tatggtcatt gcgattatga tgcagccaag caagcaaaat
     3421 agtgcagcga gtgcatttac cggcggtgcg gatcaactat tcggcaaaca aaaagctcgc
     3481 ggctttgaag ctgtcatgca gcgatcaact gcagttatgg gagctatctg gatgatctta
     3541 ttatttgtcc tagcatattt atcttcgaag tagttgaata agcctctcgc aaaacggcgg
     3601 gcggcttttt ttattgcaaa gagaacgctt aagaagaagt tatgaaagaa actatggtaa
     3661 aatgaggcga aagaggtgag acgagtgaga caattaccaa aaccccttta tgcaaaacat
     3721 ggaaagcgag cggttttgct gcttcacgct tactcaggaa gtccaaacga tgttcgaatg
     3781 ctcgcacgat ttttggaaaa agcagattat acagtttatg cgccgctttt taaaggacac
     3841 ggaacaatgg agccatatga tatcttgcaa gaaaaagcag aaagctggtg ggcagacact
     3901 aaaaaagcca tccatttttt acagtctgag cagttttctg atattgccgt attaggatta
     3961 tcaatgggcg gcatctttgc tgtacgagca ctagaagaag aatcagtgat tggcggcggt
     4021 tttttctgtt cgcctttgtc tcctgtaaaa acaaacgtgc ctgaaaactt tgaaaaatac
     4081 gtacgtcaag tgctgaagac agcaggtaaa tcagagaaag aaatcaatga aaaagcagtt
     4141 gcttaccgct cgttagctga acaacaatta atggatatac aagatcaggc agcaatcgta
     4201 gaaagtaggt tgtcagatat ccaacagccg atatttttag ctcaagctgg gaaagatgaa
     4261 atgatcgacc ctaatggcgt atttgaaact gcacgcaagt tgagccgtca gcgagtgaca
     4321 cttcaatggt atcctgaaag tggtcatgtg attacagttg gtacagcaag acgcgaatta
     4381 gaaaaagacg tcttggaatt tttagaaaaa ttaccttgga atgaggaata aatatgacaa
     4441 aagaaacgat aaaagagaaa attctctttt tcatggaaag cagtcgcaaa cgcagttttt
     4501 ctatggaaga aatcgctgaa ggtttagggt tccaaaaaag cgatgatttt aaattactag
     4561 tacaaacgat tgctcaaatg gaaagagaac agagtatagt tttcaataaa aaaggaaaag
     4621 tgaaacttcc tttaaaacca atcctgatcg aaggaacatt tcgagcaaat gaacgaggat
     4681 ttggatttgt aacgattgat cccgaagaag atgacatcta tatcccaaaa gaagcgactg
     4741 gttttgcaat ggatggagac accgtagcaa tcgatatcat gaagcgagca aatacagata
     4801 tggatcgcgg agcagagggg aaagtagtcg agattcgaga ccgggcgact actcagttag
     4861 caggggaatt cgttgcttat tcagaagaag aaatacaaga aacagatctt tacggcgttg
     4921 ttatcccgaa agatagaaag ctcaaccaat ttaaagtata tgcagcagca gagggagttc
     4981 gtccagttga cggaagtatt gtgctgatcg aactgaccca ttaccctgaa aaagggtatg
     5041 cgactagttt agaagggata atcaagcaag tgattggtca taagaatgac cctggcatgg
     5101 atatcctctc aattgtcgtg gctaatggta tccctacgaa atttcctgat gatgtattgt
     5161 ctgaagcaga tgaagttcca gatcaaatca gcgaaaagga tataactgga cgtagagatt
     5221 taagaaatca gctcattgtt acgattgatg gagaagatgc aaaagatcta gatgatgcag
     5281 ttacggttaa aaaattagat aacggtaatt attttcttgg agtacacatt gcagatgttt
     5341 cttactatgt gacacaaggc agtcaattgg acagagaagc atatgaaaga gggacaagtg
     5401 tatatttgac tgatcgagta attccgatga tcccgcaacg tttatcgaat ggtatctgtt
     5461 cactgaatcc ccatgttcca aggctgacca tgagttgcga aatggagatt gatccaaatg
     5521 gacaagtagt ctctcatgat attttcccaa gtgtgatcca aacaacagaa cggatgacct
     5581 acacagctgt caatcaaatc ttagaagatc aagacgaaga aacgatgcaa cgctatgctc
     5641 agctcgtacc tatgttccaa gaaatgggtg aattgcaccg aatccttgaa acgatgcgaa
     5701 tcaaacgtgg cgcgatttct tttgaggatc gcgaagcgaa aatccttgtt gatgctgaag
     5761 gacacccaaa tgacatccag ctaagaagca gaggaattgg tgaacgcttg atcgaatctt
     5821 tcatgcttgc ggccaatgaa acggtagctg aacattataa taaattaaat ctgccgttta
     5881 tttaccgaat ccatgaacag ccaaaagaag aaaaaatgca gcgcttcttt gattttgctt
     5941 cagctttggg cattctggtc cgaggaacga aaggaacgat cacaccaaaa gatttacagc
     6001 atgtgatcga aggagtagaa gacaaaccag aagctgcagt tatcaatact atgttgctaa
     6061 gaagcatgca acaagcacgc tattcagaag acaattttgg gcattatggc ttagcggcag
     6121 attactacac acactttaca tctcctattc gacgctatcc cgatctaatc gtccatcgtc
     6181 tgatccgttc ttacagtgaa gataaaagtg aaagcaatca aagttcgtgg gctgcggaac
     6241 ttcctgaaat tgcagatcat agttctaaga tggaacgtcg tgcagtagaa acagaacgtg
     6301 aagtagattc catgaagaaa gcagaattca tgatggacaa agtgggagaa gaattcgatg
     6361 ggatcatcag ctctgttgtg aaattcggcc tgtttatcga actgccgaat acagtagagg
     6421 gattgatcca tatcaatgaa ttgaaacagg actacttcca ttttgttgaa aatcatttag
     6481 ctcttgtagg tgaaagaact ggtctgactt ttaaaatcgg acagaaagtg cgagtaaaag
     6541 tagtcaaagc tgatccagat gaacgagcag tagattttga acttgtttcc gcagaagagg
     6601 ttacagcgtt ggaacggcca aaaacacgaa atggaaaatc aggaaaacgc gaaggaaaac
     6661 aagcaggaaa acgcccagaa agaaatggca agaagaacaa acgatatagc gatagtaagc
     6721 aaacgttcgg taagaaaaaa gggaagaagc cattttacaa aggtgttaaa aagaagaaaa
     6781 agaaaaaata aaatgagttg gaggaaacag ctatgccaaa aggcgaggga aaattaattg
     6841 cacaaaacaa gaaagctcgc catgattatt cgatcatcga cacgatggaa gcagggatgg
     6901 tcttgcaagg aaccgagatc aagtcgatac gaaacagccg gatcaatcta aaagatggat
     6961 ttattcgcgt ccgcaacgga gaagctttct tgcataatgt tcatatcagt ccttatgaac
     7021 aaggaaatat ttttaatcat gatccgttgc gcacgagaaa gttattatta cacaaaaaac
     7081 aaatcatccg gcttgaaaat gaattgaaaa atactggaat cactgttgtt cctttaaaag
     7141 tctatattcg taacggctat gccaaggtat tgattggtct ggcgaaaggg aaaaaatctt
     7201 atgataaacg ggaagatttg aaacgaaaag atatcgatcg acaaattgat cgaacattaa
     7261 aaaatttctc tagataattt cattatcctt tttcgcatat tcggtgcttt tgcttttact
     7321 taatgataaa atttgttagc atttaatctg ataaaacatg attatcatag gttttttctt
     7381 agatcaggag caccgtgtat aaagaaaaat tcgcttcgtt tttgactgaa ataaaaaagt
     7441 gtctaatttt catgaaaatg ttttgaattt tctgtttcat ggtggtatga taccgtgtgg
     7501 ttagaaaccg attggaaaaa accagatacg cacatgaatt gattttatgt gaatttcggg
     7561 gaaaagaggt gaaatggatt ggatgaacgc tcgctaacgt tccacattgg accaatttgg
     7621 tttgatggta cagtttgtat aatggtgtta ctgacctgtt tgattgtttt cttcttagct
     7681 tactttttta caagaaatct taagttgaaa ccaacaggca aacaaaatgc gatagaatgg
     7741 gtcattgatt ttagtcgagg aatcgtaaca gataatctgc ctagaaaaga attgagcaat
     7801 ttccatttgt tggcatttac attgttttta tttgtttttg ttgcaaacaa catcggttta
     7861 gtaacaaaaa tcgttttacc taacgaaaca acactttgga aaagcccgac agcagacccg
     7921 tttgttactt taactttggc atttataatg atcttactta ctcacttatt cggggtgaaa
     7981 aagttaggct tcaaaggata tttcgtaaat tcgttcttga aaccctatag ttttatgttt
     8041 ccgatgaaac tgattgaaga atttacaaac ttattaacac ttgctttacg tctttatggg
     8101 aatatttatg cgggggaagt tttgctaacc ttgattgcaa aaatgatgac aagcttaggc
     8161 tggttctcat taccattagc aattccatta gagatggtct ggatcgcgtt ctccttgttt
     8221 attgggagca tccaagcatt tgtctttgtc actttgtcaa tggtttatat gagccataag
     8281 attgaagtag aagaataata ctcggttaaa aaacaaacta ctatttatct attttaggag
     8341 gaaaacaatt atgaattata tcgcagcagc aatcgcaatc atgggagcag caatcggagc
     8401 cggttatggt aacggtcaag ttatctctaa aacaatcgaa tcaatggctc gccaacctga
     8461 aatgtctggt caattccgta caacaatgtt tatcggggta gctctagttg aagctgttcc
     8521 tatcttaggg gtagttatcg ccttgattct agtttttgcc gtttaattga atcagtatta
     8581 aggcagaaac aacatcgttt gttactgctt ttttacatgg aaactagaaa tactccaaag
     8641 aaaggaaggc tcgcctatgc tgaatcaatt ggcaattgca gaagttggca atgcgatgtt
     8701 aggtaatatc atcgttgtca gcggctcatt tttgatacta ctggctctct tgaagcactt
     8761 tgcttgggga ccaatcagcg atattttgaa aaaacgtgaa gacaagatcg ccaatgattt
     8821 agattctgca gaacaatctc gcatcaactc agcgaaaatg gaacaagaac gcgaacaaca
     8881 attgttagcc tctcgttctg atgcagctga tatcatcaaa aatgcgaaag aaagtggaga
     8941 attaagccgc caaaatattt tgaaggatgc tcaagaagaa gcagctcgtc taaaaagcaa
     9001 agcccaagct gatatcactg tagaacgtga ttcagcgctg aactctgtaa aagacgacgt
     9061 tgcagaactc tctcttcaaa tcgcggaaaa aatcttgaac aaagaattat ctccagaaat
     9121 gcatgaatca ttaatcaatc aatacattga aggtctaggt tcttcaaatg aaactagata
     9181 aatatacagt aggaagacgt tacgggaaag ctttatttga actggcgatc gattcaaatc
     9241 aagcagaaga aatctatcaa gatctcgtaa gtttacgtca aatctatgaa caagtacctg
     9301 gattagggaa tatgttaagt gatgttcgtc tagaacctca tgaaaaacga gaaatcatgg
     9361 acaagcttgt cagcggttat gatgggatcg ttaaaaactt tttagaagtt gtttatgagt
     9421 ataaccgaat ggatgacctt ttgttcatga tcgaagaata tgaacgtcga tatgatgaac
     9481 atcaagggtt actattggga agtgtcacaa cagcagtccc actttcagac gaacaacgaa
     9541 tacaattaga aaagaatgtt gcaaaaacaa tggactatca atcagttgaa ttaaaacaaa
     9601 tcgttgattc ttctattatt ggtggcgcga ttgttgaagc caaccatcga gtaatcgacg
     9661 gaagcattcg cacgcaatta gaaaaaatgc gtaatcaatt aaacagatag tacaaagagg
     9721 taggtgaatc gaatggccat caaagcagaa gaaattagtg ccttgattaa agaacaaatt
     9781 gaaaattatc aaaacgtgct ttcagtcgaa gaaatcggta ccgtaactta tgtaggtgac
     9841 gggatcgctc gtgcacatgg tttagaaaac gcgatgagtg gtgagttgct tgaattttca
     9901 aacggctcat acggaatggc gcaaaactta gagtcaaatg atgtaggtat cattatcctt
     9961 ggcgatttcg agtccattcg tgaaggagat aaagtaaaac gaacaggtaa gatcatggaa
    10021 gttccagttg gagaggcctt gatcggtcgg gtagtcaatc cgctaggtca accaatcgac
    10081 ggactaggtg aaatcgttac tgacaaagct cgtccagtag aagcgatggc accaggtgtt
    10141 atgcaacgta aatctgttaa cgaaccaatg caaacagggc taaaagcgat cgacgccctt
    10201 gtgccaatcg gacgcggaca acgtgaatta gtcatcggtg accgtaaaac agggaaaact
    10261 tctattgcga tcgatacgat catcaaccaa aaaggtcaag atatgatctg tatctatgta
    10321 gcaatcggac aaaaagattc tacagttcgt acacaagttg aaacattgaa aaaatatggc
    10381 gcaatggatt acacaatcgt tgtgaatgcc ggtgcgtctc aaccagcacc attgctttat
    10441 atcgcaccat atgctggtac tgcaatgggt gaagaattca tgtacaacgg taaacatgta
    10501 ttgatcattt ttgatgatct ttcaaaacaa gctgttgctt atcgtgaact ttccttgcta
    10561 cttcgccgtc caccaggtcg tgaagcttat cctggggatg ttttctactt gcactcacgt
    10621 ttattagaac gtgcagcaaa attaagtgat gatttaggtg gcggttcaat gactgcctta
    10681 ccatttgttg aaacacaagc tggagatatc tctgcttata tcccaacaaa cgttatttcg
    10741 atcactgatg gacaaatctt cttagaaagc gacttgttct acgcaggtac tcgtccagcg
    10801 gttgatgccg gactatctgt atctcgtgta ggaggttctg cgcaaatcaa agcaatgaaa
    10861 aaagttgctg ggactttacg cctagactta gcaagttacc gtgaattaga agcatttaca
    10921 caattcggtt ctgacttgga tgcagcaaca caagcaaaac taaatcgtgg acgccgtaca
    10981 gtagaaatct tgaaacaaaa attgcatgca ccgctagctg ttgaaaaaca agtagtgatt
    11041 ttgtatgctt tgacacatgg attcttagat agtatccctg tagacagtat tttagacttc
    11101 gaacatgaat tattcgaata tctggatacc aatcattcag atatctttga aacaatccgt
    11161 acaacaaaag atcttccaga agaagaagcg ttgaacagtg cgattcagga atataaagac
    11221 atgttcttag ctacaaaagg aagtaattcc tctacagaag ataaattgaa atccattcaa
    11281 aatgcatagt gaggtgagat gaatgggtgc ttcattaaat gaaatcaaga cacgtatcgc
    11341 ttcaacgaaa aaaacgagcc aaatcacgcg ggcaatgcag atggtgtctg cttctaagtt
    11401 gacaaaatct gaagcttctt cacagaagtt tcaggtttat gcaacaaaag taagagaaat
    11461 cgttacgcat ttgactgcta ctcagttaaa tgatattgcc tcagaaaatc ctagagggga
    11521 tatcaattac aacagcatgc tgctcagccg tcctgtgaag aaaactggtt atatcgtcat
    11581 cactgcagat ggcggtttag tcggtggata taacagctcg atattgaaac aaacgatgtc
    11641 tattttagaa gaagaccacc aatcacccga tgattacgtg atgatcgcaa ttggcggaac
    11701 tggtgcagac ttctttaaag cacgcggtat caatctagca tatgagctgc gcaatctatc
    11761 tgatcagcca agctttgatg aagtccgcaa aattgtaaat atggcaacaa ctatgttcca
    11821 aaacgaagta ttcgatgaat tatacgtatg ttataaccat catatcaatt cgctaactag
    11881 tcagtttcgt gtagaaaaaa tgctgccaat ctcagactta gatccagaag aagcaacgac
    11941 tttcgaacaa gaatatattt ttgaaccttc aaaagaagaa atcttgtctc agttattgcc
    12001 tcagtatgct gaaagtttga tctacggtgc gatcgttgac gccaaaacag cagaacatgc
    12061 tgctggtatg acagcaatga aaacagcaac tgataatgct gctaatatca ttgatgagct
    12121 gacggtttct tataaccgtg caagacaagg ggctattacc caagaaatta ccgaaattgt
    12181 tgccggagct tcggcactag aatagtattg agattggagg aaaaaagatg agttcaggca
    12241 agattgttca agtaatcggt cccgttgtcg acgtggaatt ttctttagac caatccttac
    12301 cagatatcaa caacgcatta gttgtttata aaaatgacga aaataaatca aaagttgttc
    12361 ttgaagcagc gttagaatta ggggacggag tgatccggac cattgcgatg gaatcaacag
    12421 atggtttaca aagaggaatg gaagtcattg atacaggcaa agcaatctct gttcctgtag
    12481 gtaaagaaac attaggtcgc gtattcaacg tactaggaga tacgatcgat ttagaaacac
    12541 ctttcccaga agatgcggaa agaagcgaaa ttcataaaaa agcaccagcc tttgacgaat
    12601 taagtacaag tacagaaatt ttggaaacag ggatcaaagt tatcgatttg cttgccccat
    12661 atttaaaagg tgggaaagtc ggactattcg gtggtgccgg tgttggtaaa accgtactga
    12721 tccaagaact gatccataat atcgcccaag aacatggtgg tatttctgta tttaccggtg
    12781 taggtgaacg tactcgtgaa ggtaatgact tgtattatga aatgaaagat tcaggagtta
    12841 tcgaaaaaac agccatggtg ttcggacaaa tgaacgaacc accaggtgca cgtatgcgtg
    12901 ttgctttgac tggtttgacg attgcggaat atttccgtga tgtagaaggt caagatgtac
    12961 tgttgtttat cgacaacatt ttccgtttca ctcaagctgg atctgaagta tcagccttgt
    13021 taggacggat gccttctgcg gttggttatc aaccaacatt ggcaacagaa atgggtcaat
    13081 tgcaagaacg tatcacatct acgaaaaaag gttcgatcac atcgatccaa gcaatctatg
    13141 tgccagccga tgactacact gacccggcgc cggcaacagc attcgcccat ttggatgcga
    13201 caaccaactt ggaacgtaaa ttaacagaac aagggattta tccagcggta gatccattgg
    13261 cttcttcttc aagtgcttta gcacctgaaa tcgtgggaga agaacattat aaagtagcaa
    13321 cagaagtcca acacgtattg caacgatatc gtgaattaca agatatcatt gctatcttag
    13381 gtatggatga attatccgat caagaaaaag tgttagtttc tcgtgcccgc agagtccaat
    13441 tcttcttgtc acaaaacttt aacgtggctg aacaatttac tggtcttcca ggttcatatg
    13501 tccctgtaga agaaacagtg aaaggattcc gcgaaatctt ggaaggcaaa tacgatgatc
    13561 ttcctgaaga agctttccga agtgttggcc gaatcgaaga cgtcgtagaa aaagcgaaaa
    13621 cactaggcta ctagaaaggg gtgccccatg gatcaatatt taacagtaaa tgtggtaact
    13681 cctgccggtt tggtctatga tcaccatgca gctatcgtcg ttgcacgtac gacagctggt
    13741 gagcttggga ttttgcctaa acatgcacca atcatcgttc cattgacgat tgatgaagtt
    13801 cgtgtaaaac gaacagattc agatacccac gtcgattgga tcgctgttaa tggcggaatc
    13861 atggaagttc gtgataatgt tgtttcaatc gttgctgata gtgcggaacg tgaacgtgat
    13921 atcgatgtat ctcgtgcgga acgtgcaaaa caacgcgcag aacgccaaat cgaagaagca
    13981 aaagcaaaac aagatacgaa cgaattgaaa cgggcaactg ttgcgttgca ccgagcaatc
    14041 aatcgtatca aagtatctaa gcactcataa aatgagatgc ggatgtttat ccgtgtttaa
    14101 gaacagtaaa aagaggttat gacagaagtg gtcagcttca agacataaga aggaatttac
    14161 gaaaattgct tctcaaattt ttgtgaaatc tcagcttatt tttcgaagaa gctacttctg
    14221 gaacaccgta tatcagaggt tgtgaaatca gcgttttgtc ctgagtatta gagaaacaaa
    14281 tggaaaatag ttcctcatat tttgccgttt tttgagctaa taccgagggt cagatgattg
    14341 aacaccgttt atttggaata agggactgcg acaagacttt tgtctcagtc cctttttttg
    14401 catattttta gcatttattt cctaaaaaat gtaaggggat tcctatattt ttaagtatac
    14461 ttgtttttcc ttttatacat tttatggtat tatcatttta ttagttatta taaaacaatc
    14521 gttagaaagg acgtcattat gcaagtatac ggaattgatg cggtgcttcg aatcgtgagt
    14581 catttggcgt ttatctactt agccttctgg tctttacgtt cgttacgcat tgaaactttt
    14641 ttcagaccaa tgcataatac gcaaattaga atggcaatcg tacttttttc aattttttta
    14701 gggtacactg caagtaattt tttcttagag gttatcgctt tatgccgaaa tcttttcgtt
    14761 agcctgtaaa ttttaaaaaa aggtactatt ttaaaagcaa ggatgttata atagaaaaga
    14821 ttttgagtaa gtgtatgctc attggaattt tttggaggga acaacatgga agagatcatc
    14881 gtaagaggtg gcaatcaatt aaatggaaca gtaagaattg aaggagctaa aaatgctgtt
    14941 ttgcctattt tagctgctag tttgttagca gaagaaggta ttacgacatt agacaacgtt
    15001 ccgattcttt ctgatgtctt cactatgaat caagtgattc gtcacttaaa cgtagatgta
    15061 gattttgatg aacaaaagaa tcaagtaaca atcgacgctt caagacaatt agaaatcgaa
    15121 gcgccttatg agtatgttag tcaaatgcgt gcttcaatcg ttgtgatggg tccgctactt
    15181 gccagaaatg gacatgcaaa agtagctatg cctggtggat gtgcgattgg taaacgacca
    15241 atcgatttgc atttaaaagg atttcaagct ttaggagcaa aaattattca aaaaaacggc
    15301 tatatcgaag caattgcgga tgaattgatc ggtaacacga tttatttaga tttccctagt
    15361 gtaggtgcta ctcaaaatat catgatggct gccgtaaaag caaaaggaac aaccatcatt
    15421 gaaaacgtag ctcgcgaacc agaaatcgtt gatttagcaa acattttgaa taaaatgggc
    15481 gctcaagtct atggagctgg tactgaaaca atgcgtattg aaggcgtgga tcatttacat
    15541 gcagtcaacc actcaatcgt acaagatcgt atcgaagcag gtacctttat ggttgctgct
    15601 gcaatgacac aaggcaatgt gctgattgca gatgctatct ctgaacataa ccgcccattg
    15661 atttctaaat tgatcgaaat gggtgcagaa atcattgaag aagaaggcgg agtccgtgtc
    15721 atcggaccaa aacatatctt acctacagat gtaaaaacga tgcctcatcc tggtttccct
    15781 acagatatgc aagctcaaat gacagctatc caattagttg cagaaggtac aagtgtggtg
    15841 actgaaacag tgtttgaaaa tcgtttccaa catttagaag agatgcgccg tatgaacgct
    15901 catgtcaaaa tcgacggaaa tgtagcaatc atggatggaa accatgaatt gcaaggtgca
    15961 gaagtatatg caacagactt gcgggcagca gcagcattag ttttagctgg cttgaaagca
    16021 aatggaatca cacgtgtgcg taacttgaat tatctagacc gtggatatta taatttccat
    16081 atcaaactac agcaattagg tgcagacgtc gaacgtgtag acatggatca aacttctgct
    16141 gaaaaaacag cacaaacgat tgcttaagga agtggctgaa tgagtaacca acgatatatc
    16201 ttattaactt tgataaagat tttagtggtg atcttattgc ttatccttct ttttgtggcc
    16261 ggaacgatga tcggatatgg tgtcatcggt ggcggcaatc catttaaagt attccagcca
    16321 agtttgtgga ttcatattcg agactttttt cattgataaa taagaatcac agtggttcta
    16381 gtcgatatga agtgagccat gaagcagaac atgataaaag aaaacagaag ggaacgttga
    16441 cgttagtcgc gatccctttt tttagtaaaa gaaaagaagg aatctaattg atgaagaaga
    16501 tattgattta tttagttaga ggatatcagc gctttatttc gcctgcattt ccacctagct
    16561 gccgttatta cccaacctgt tccaattata tgataaaagc aattcaaacc catggtgcgc
    16621 taaaagggac ggtaatgggg actgcacgga tcttacgctg tcatcctttt gtcaaaggcg
    16681 gtatcgatta tgtaccattg acattccgat taacgcgaaa taagaaagat aaaaaagaac
    16741 cagattataa aaaggttgtg aaaaatacgg gtagcactga gcgataaaga aaaagaggaa
    16801 atttattgtc aaagtaacaa ctccgcaact acttatatgg gggacaaaaa atgatcgaag
    16861 aactaaacaa gaaagattta ccatggtcct tgttattaga tgcagatcct gataaagaaa
    16921 aagttcaagt atatgtttcc agagggagtg gctggatatg gaaagaaaaa gaaaaaacaa
    16981 taggtgtact gatctatgtg gcaagggaaa aagaatacga aatcgtcaat gtagcagtaa
    17041 ccccaacgta tcaaggaaag gggattggcg gaaaattact tgagacagct tttcagaagc
    17101 tttcacagct agcttcaaac caaactcgga tcattatacg tactggcact actagtagcg
    17161 ctgcgttaca cctctatcaa aaaatgggct atgtggagat tggtcgagtc aaagactatt
    17221 ttatccatca ttactctgag cccatttttg aaaatggaga tcaattaagg gatcaagtga
    17281 ttttagcaat tcacttacct aaaaatagct ttaactaata aactttcaat tcgagaaaat
    17341 gctttcaaat gctaaataga tacattacaa tagctttagc aaacaaaaag gagaatattt
    17401 ttatgggaat attagttttt gattttggtg gatcggctgt caaatatggt tgttgggatg
    17461 gcaaagagat taaaggtaag ggacaatttg caacgcctga atcttgggaa gaaatgaaaa
    17521 cgcaattatt tcaagtttat aaaaaaatac tcgtttcttt tgaagtcgaa ggaattggaa
    17581 tcagctctcc tggagtagtg aataatgaga aacaggtgat tgagggaatc agtgcgattc
    17641 cttatattca tggatttaat attttccgag atttggaaac tctgtttcaa ctcccagtaa
    17701 cgattgaaaa tgacgccaac tgtgccggaa tggctgaatt ttatgaaggt gctgcccgtg
    17761 attatcaaga tgttgctttt gttgtggcag gaactgggat tggcggggcg ttgttcacga
    17821 aaggacaaat aaataaaggg gcacatcttt acggtggaga atttgggttg atgttcttag
    17881 atggcgataa gacattcagt aaacttggta cagcggtaca gatggcttgg cgttattgtg
    17941 aacgcaaagg tctcgataaa aatgcttata ccggaaaaga tgtctttgaa ttagcagaaa
    18001 ctggagatgc aatagcaaaa gaagaagttg agagttttta tacgtatctt acaaagggat
    18061 tattcagtat tcaattttcg tttgatccag aagtgatcgt attaggcggc ggggtttctg
    18121 caaaagaagg gctcattgat gagatcaaaa gccgaatgaa gaagctgaca gaacaattcg
    18181 atctgcatga tttcgaaccg cagattttat tatgtgagta cagaaacgat gctaatctta
    18241 taggtgcagc agcaaactat ctcgctgtac gtcaaggaga gaattaagag ctttagagaa
    18301 ggtgcagctt gcataaaaga agagaatgaa atgaatagga gatttttacc taaggaatgt
    18361 tttatttgct gcagtatgat aaaatatctg agaggtgaaa atgatgtcaa aaaaaaataa
    18421 agatattgaa gtgcgtatag aagaagtgaa aaaagatatc aaaggtaaga attatgaagt
    18481 cactcagtta tttataggga aaaaaatgat tggtgagatc ttagcctatg ggccaaaaga
    18541 atatgaaatt ttctttggtg aagaagattt tggaaaagaa aaaagtcttg aaaatgcgat
    18601 agaaacagtg attcgacact ggaatctgca cgaataaaaa aatatcaaaa aaactttcaa
    18661 taaagggttg caaaaatctg gaaaattagg tattataact aagtcaggtt gatacaaacc
    18721 tttcggagga gtagcgaagt ggctaaacgc gacggactgt aaatccgttc cttagggttc
    18781 agtggttcga atccactctc ctccatttca ttggggtata gccaagcggt aaggcaaggg
    18841 actttgactc cctcatgcgt tggttcgaat ccagctaccc cagtatacca caatcgtaag
    18901 ctctcgttag ttcgagagct cttttttttt gctttgctac cttgttcctt gaagattctg
    18961 ctgaattaag atagagttaa gttaactaca agaattggag ggatccacat gaaatggaaa
    19021 gaaaaagtag acgaagcagc tgaaaagtta tatgatttgg tcaaatcaga aaaatacaat
    19081 attgaagtga atattccgaa aaaaggcgga aaagcggtcc aggtaaaatc aaaacgccca
    19141 acgaatcata caaaaaaatg gatggctaaa aaccgctaat aataacaaaa gatccataag
    19201 tcggcatttt catcgatttt agggtctttt gttattttct aggttgaaca tatctaaaaa
    19261 attgtttctg acaataggag tagggagaac gatgtgtgtc taaatcctct gttgatggat
    19321 agaaaccttt atctttttca gttgtgaatc aggtatatac cagttataat agaagaaacg
    19381 aaaggacggg tgtacgttat gtcgaatcaa ttacctgtat atatacagat tcatgaccaa
    19441 ttaaaaaatg aaatcgaaca aggtatatgg aaagtcggag accgtcttcc ttcagaaaga
    19501 gaattagcag tgaaattcaa tgtcagccgt atgacgttga gacaagcaat tcaaaacttg
    19561 gcagatgaag gaatcttaga aagaaagatc ggttcgggta cgtatgttgc gcgagaaaaa
    19621 gtacaagaaa aaatgtctgg tgcaacaagt tttacagaaa ttatggaatc acaaaatcga
    19681 gtaccgtcta gtcggacaat ctcttatttc ttgacttcgc caagctcaag tgaaatggaa
    19741 aaactaaact tgagtaaaga tgacacgatc ttgcgaatgg aacgtattcg tttcgcagat
    19801 gatgtcccta tttgttttga agttgccagt gttccacaaa agctgatcga tggctacagc
    19861 aaagcagaga ttactgaatc gttgtatcgc acgctagaag aaaaaggcgg gaaaaagatc
    19921 ggtccagcga atcaaacgat tactgctgtg ttggcttctg agaaaattgc tgactattta
    19981 gaggtaaaac gaagagatgc agttcttcgt ttgcgccaga tttcttattt tgaagacggt
    20041 acgccgtttg agtatgtgcg tacgcaatac gtaggaaatc gttttgagtt ttatttggaa
    20101 aaataaaaaa caggtgatcc ctgattcctt attagaaata aaagagaatc agaatcacct
    20161 gtttttatat atgatcagtt atcagttgct cgtgataaga aacagaaccc tcttcaaatt
    20221 ggacttgtcc gttcagcagc tcaatgatgg attctttaaa gtggttagca cttttttcat
    20281 ctacagcaca gatgacgcta acttgatcag tatattggat ttcttttata aaaaaatgtt
    20341 ggagttcaag ataattctgt aattttccta gttgcggata cgtgatcatc acagagatct
    20401 cctgttgcaa ggtaccttct acgattccaa tgtcagacaa tgcttgagag accgcatgag
    20461 tgtatgctcg tattaatccg cctgtgccga gtttcgttcc tccaaaatat cgagtgacta
    20521 ctgcagctac attgatcagt tgattttttt tgaggacttc caacatcggt acgcccgccg
    20581 ttccacttgg ttctccatca tcgctgcttc gctggatttc atttttttct ccaatgatga
    20641 aggcgctgca gttatggttc gctttccaat gttctttttt tatttcttga atgaaatttt
    20701 ttgcttggtc ctctgattct attcgtctaa gagaacagat aaagcgtgat tttttgatct
    20761 caatctcact ttgtccattt tcttttattg tacgatagcg ctcgatcatc ttgtcacctc
    20821 tttttttcat tttctttatt cagtataaat aaagttctag gttgtttcaa cactgtaacg
    20881 gttgcgcaat tgctgtagta tgcgataata aagacaaagg caggtggatc tggggatgca
    20941 ggaacgaatg aaaaagtacg atgcaattac gcattatttg aagaacaatg gcggctctca
    21001 agtaacgttg acctttaccc aatttgacga actgctgttt cccagtaacg gacttcccaa
    21061 aacagcaagg gaaagtacag attggtgggc aaatgattat aaacaccctg aaaaaggtgc
    21121 ctatggctgg ataaatgcag gctatgaagt agttgtcatc aatttggaca aagaatatgt
    21181 cgtgttcaat aaattagtga aatctagttg gctatttgat taaataaacc gcgtttagaa
    21241 aacgttgaca aatttctttc tttttatata ataaagtaac agacgcgaag aaaacgtttg
    21301 cgtagtttgt tttaaaaagg agagaaaaat atggctaaaa aaacaatcat gcttgtatgt
    21361 tcggctggta tgagcacaag tttactagta acaaaaatgc aaaaagctgc tgaagcaaaa
    21421 ggaatcgact ctgatatttt cgctgtgtcc gcttctgacg cagataacaa tcttgcaaat
    21481 aaagacgtgg atgtgttgtt attaggccca caagttcgct ttatgaaagc tgattttgag
    21541 aaacgtctgg aaccaaaagg cattccttta gatgtcatca atatggctga ttatggtatg
    21601 atgaatggcg aaaaagtgtt agatcaagca atcgcgctca taggtgaata aatagaacag
    21661 tataactatc gaacaagcga tccaaaaaat agatcgcttg ttttttcgta tattttttta
    21721 gggcgaataa tatgaatcat ttttggggta gaaggcttct gacgagagat attaaagcaa
    21781 tagactgtga ggaggtaaat ttaccaaatt atgagaagat cgcagcagtg gaacgtttgg
    21841 gcaactggat tctttgtcat cgctgtggaa tgaggacgtc cgtcattgtt ggacagcttg
    21901 cagattacgg ttatttttgt acacattgtc tatatttagg gtgatgcgac actcaacaag
    21961 atttatattt gtttgatcag cctgaagcaa aacctcgaga agtcgtcttt ttttggacag
    22021 gacagcttac tgaaaaacaa acggaaatcg caaagagaat actttgccat tcagaaaaaa
    22081 gacatcatct tatttgggct gtaacaggtg cagggaaaac agagatgctt tatcctattt
    22141 tagtggaaac tttaaaagct ggaggaagag tagcgatttg tacaccgcga gtcgatgttt
    22201 gtaatgaatt gtttttacgc tatcgttatg tttttccaga agaaaaaatc acgttgctcc
    22261 atgggaatac agcaacggct tataccttta cttcttttgt tatctgtacg attcatcagc
    22321 tataccgttt ttatcggtcc tttgatttaa tagtgatcga tgagatcgat gcgtttcctt
    22381 ataacgggga tgctgggcta gctcacgctg tgcaaacagc aatcagatca aacggccgat
    22441 tcatctactt aagtgccacg cctgatgaga agttactaaa ggaaatcaag cagacatttg
    22501 agctccacaa acttgccttg cgctttcatc ggcgcttgct tcctgaaccc gtgcttgttt
    22561 tcatgaataa ttggcgacaa aaatgtggaa acaaaaagaa aatcaactct ttggtgcgga
    22621 tcattcagca actaatcaaa gaaaaccgta tccttttatt ttgtccaagt attacgatga
    22681 tgcatcgttt aaaaatcaca ctgcaagaag cttttccaga atatgagata acagatgttt
    22741 cttcacaaga cgatcaacgg gcaaagaaag ttcagaaaat gcgagaagca atatacgaga
    22801 tactgttaac cacaatgatc ctagaaagag gagtcacatt tgaaagaatc tctgtcatcg
    22861 ttttaggagc agatcatcca gtttttacta aatcctcttt ggtgcaaatc gcaggaagag
    22921 ctgatcgtaa aggtccttat acgaacagtc gagtctattt tttctatgaa gaaaaaactt
    22981 cagctattcg aaaagcctgt caggaaatca aagaaatgaa cgaagaaggg aagcagatgc
    23041 tgtgagatgt agttgctgcg gtaaagacat cattcaaaat ttaacattca gcgagatcat
    23101 cggagtgtcg aaatctagac ttcctcagac atgttcagtt tgccgcgcac aattccattt
    23161 attagaagca aaaggttgtg caggttgtca aaaaccaatc catttgaaat acgaggagac
    23221 gaaaaagaaa gcaaatacta gtttcgttta ctgcaacgac tgtcaagaat ggaagaagaa
    23281 atatcctgca tatgattttc aacaccgcgc cttttttacg tacgatgaaa agatgcaggc
    23341 ttggatcaat cggtacaagt ttatgggaga tatccgatta gcagggactt ttgcatataa
    23401 atggactcag ataaaaaaag attacccggc atatatttat tgtccaatac ctttatctga
    23461 aaaaaggtgg ctagaaagag gattcaatca agtaagcgag atgttgaaag cagcaaaaat
    23521 tccttttcag atgctgctca aaagagagca acatctgctt gctcaagcaa aaaacacgcg
    23581 aaaggaaaga ttggagatgc ctcagccttt tgagcttgcc gtacctcttt ctaagataca
    23641 acataaaaaa atattgatta ttgacgatgt ctatacaaca ggacagacgc tttttcatgc
    23701 agcagattgc ttgcagaacg cacagccgaa aatcatccga accatttctt tggcaagatg
    23761 aatcagatgt ggaaatttga tagcgattta atttgcaaat aacctagagt tcgatataat
    23821 agaattaaga agagggaaag agtggtcctt ctccttcttg attgtggtag acgaaagggg
    23881 taatttttat gtttagatat aatgtacgcg gcgaaaatat cgaagtaact gaagcgattc
    23941 gcgactacgt tgagaaaaaa gtaggcaaac tagaacgcta tttcagtgac gcacccgatg
    24001 caacggcgca tgtgaactta aaagtttata cagaaaaaac agcaaaagtg gaagtaacga
    24061 tcccattacc ttatcttgta ttacgtgccg aagaaacttc tccagattta tacgcaagta
    24121 tcgatttggt tgttgacaaa ttggaacgcc aaatccgcaa attcaaaaca aaaattaatc
    24181 gtaaatcccg tgaaacgggc atgaatactg cagatgctgc tgctttattc aatgaagagg
    24241 acgaaaacga aaatgattct gaattagata tcgtacgtac gaaacgcctt tcattgaaac
    24301 caatggatag tgaagaagca gtcttacaaa tgaacatgct gggacataac ttctttattt
    24361 ttgaagatgc agaaacaaat ggtacaagta tcgtttaccg ccgtaaagat ggaaaatacg
    24421 gactgattga aacagactga tcagccttac ttaaataaca acgaaaactt gcagcagcta
    24481 ctgacagctg ccgcaagttt t
//
LOCUS       DASHHB010000036        23827 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.037, whole genome shotgun sequence.
ACCESSION   DASHHB010000036 DASHHB010000000
VERSION     DASHHB010000036.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23827)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23827)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23827
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.037"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(53..787)
                     /locus_tag="U2K71_001720"
     CDS             complement(53..787)
                     /locus_tag="U2K71_001720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin family protein"
                     /protein_id="HEM7315800.1"
                     /translation="MDYSLMTRLAAEMIGTALLIVLGNGAVANVDLKGTKGYGSDWML
                     IAVGYGFGVMMPAMIFGGISGNHINPAFTIALASNGLFPWAEVVPYIIAQLIGAAIGQ
                     LIVVACYKPYYDQTEDVNHVLGTFSTINSVGSKFNGFVNEFFGSFVLFFGALGITKAP
                     FFQDNLGTAHLALGFLVWTLVASLGGPTGPGLNPARDFAPRLVHALLPLKHKGSSQWG
                     YAWVPVVAPICAGVVAVFLYKLLFMA"
     gene            1114..1524
                     /locus_tag="U2K71_001721"
     CDS             1114..1524
                     /locus_tag="U2K71_001721"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CidA/LrgA family protein"
                     /protein_id="HEM7315801.1"
                     /translation="MKFFQEFFIIIFFSVLGEGIRIFAHLPIPGSILGILFLFLAFEM
                     HLVDPEKIGATGDFLLNNLAILFVPAGVGLLEYFDDIASIWPVLLGAVVVCSIVTMVA
                     AGKTAEGVEALLGYVRKKKAERVSVNEMEENQID"
     gene            1517..2218
                     /locus_tag="U2K71_001722"
     CDS             1517..2218
                     /locus_tag="U2K71_001722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005880671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LrgB family protein"
                     /protein_id="HEM7315802.1"
                     /translation="MTEFFVNPLFGLSLTLGVYVLSDKFLRKYKLPVLNPLVLSIVVI
                     ILFLHFSGISYKDYNIGGNFLTMLITPATVALAIPLYKNFRLLKENFYPVMAAILAGI
                     VANCLVSVGFGYFFSLHKEMVISLLPKSVTTAISVDLSHTMGGVNAVTLAIVVSTGIF
                     GSLIATHIFRLFKIESPVARGVALGSTSHAIGTAKAIEIGEIEGIISGLAICVNGILT
                     VLLLPLFFQPFAGLF"
     gene            2218..3804
                     /locus_tag="U2K71_001723"
     CDS             2218..3804
                     /locus_tag="U2K71_001723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315803.1"
                     /translation="MRKPLFYATITKTFEHKKGGKDVYLSSEYLKQFDKDLYYKILLL
                     ESFEDQAWHTAAQLAQVVQLDARSVSKYLNELSKNYQQFSGKTHPLFTKNHRSGYNFY
                     DTLDSIEHERFLIYLVQSTLKFQLLHDIFFEEFHTMYQFAQKHYISESTAHRKINEWK
                     QQLQTYGIRLQRGTYIAQGEEEIIRLYLHMTFWQLFRGKIWPFETISQMDVKNMAEHI
                     MAFFNVRLNEIKKRRLEYMLGAFFLRKSQKHYVVLNEKKRRLISDNLLFQRFCQVMEP
                     VFPNYFQVEDELGALFLVLMTREEYYSDPKIRKKIFDFHQQAKTPPFTALSEAKAALS
                     LYQEEQGLPAENLTFEAENYLFSSHFFAYLFPNAKETIDGNSSDFINHLVIENKELKQ
                     WLVHFFESRHKHPNHLAFKNHAFLMGRYLTVFKTLGAFTPQLPKITILLMTDFPLFEE
                     QLLEEGLRTFFRNEYQLIFLPTDYRGREVDLLISTSKVHRKPWADLDYFIVTEELKLI
                     DYIQLSQKFEMIQKQKLLKQ"
     gene            3823..4044
                     /locus_tag="U2K71_001724"
     CDS             3823..4044
                     /locus_tag="U2K71_001724"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016186310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2829 domain-containing protein"
                     /protein_id="HEM7315804.1"
                     /translation="MTFEEILPELKKGAKIIRKGWSGFELYVVLVSDDEFDGCPVTPY
                     LLIKTSDEGFSSFAPTVCDILADDWAIVE"
     gene            4041..4802
                     /locus_tag="U2K71_001725"
     CDS             4041..4802
                     /locus_tag="U2K71_001725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase"
                     /protein_id="HEM7315805.1"
                     /translation="MKNQYPDLAKQVVFITGAASGIGAAQARAFLEQDAFVFGLDQKF
                     GKMEEISLRFPETFAYALGDVRQMKELQQAVSKCQNHFGEVTILLNTAGILDAYKPLM
                     ETDESLWDLIYETNVKSMYQLTKIILPDMIRQKSGTIINMASIAGLIAGGGGIAYTSA
                     KHAIVGFTKQLALDYAGQGIRVKGIAPGAIQTPMNAADFEGKGEMAEWVANETPVKRW
                     AQPEEVAELTLFLASPQASYIQGTIIPIDGGWLLK"
     gene            5182..5691
                     /locus_tag="U2K71_001726"
     CDS             5182..5691
                     /locus_tag="U2K71_001726"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="QueT transporter family protein"
                     /protein_id="HEM7315806.1"
                     /translation="MEKNNTRSAKRWTTTDVTRMALVTGLYVAVTLVLSVFSFGVIQI
                     RLSEMFNYLSLYNKRYIVAVTAGVALANIASPLGLIDVAVGSVSTLLVLLINYKITSR
                     IKNMKIKMAVTACVFAFSMFTVAGQLTILYQLPFFLNWWVIALGELASMIVGGIIIYW
                     IGKKVDLSK"
     gene            complement(5766..6305)
                     /locus_tag="U2K71_001727"
     CDS             complement(5766..6305)
                     /locus_tag="U2K71_001727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HEM7315807.1"
                     /translation="MNRTLKELILAAEFAAIISVLSQMTIPFGLVPLTGQTFAIGLTA
                     TFLGRKLGLISVGVYLLLGLIGLPVFSGMTGGIAVLFGPTGGYLIGFLLQTWITGWMI
                     ERTDAHYLYAIFANLMGSLAALICGTIWLKISGDLTFTTAFASGLLPFLLPEAIKATG
                     AAYIGILMKKRLNRFLHTN"
     gene            complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="U2K71_001728"
     CDS             complement(6445..7056)
                     /gene="rpsD"
                     /locus_tag="U2K71_001728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HEM7315808.1"
                     /translation="MSRYTGPSWKISRRLGISLSGTGKELARRPYAPGQHGPNSRGKQ
                     SEYGMQLTEKQKLRHMYGMNERQFRNLFVKASKIKEGKHGVNFMILLEQRLDNVVYRL
                     GLATTRRQARQLVNHGHILVDGKRVDIPSYHVEVGQVISVREKSQNIVTVKEAVEATV
                     GRPAFVSFDAEKLEGSLTRLPERDELYPEIDEALVVEYYNQSL"
     gene            7318..8169
                     /locus_tag="U2K71_001729"
     CDS             7318..8169
                     /locus_tag="U2K71_001729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite transporter family protein"
                     /protein_id="HEM7315809.1"
                     /translation="MRGYVQMEKSEGSLRRKSMNPVSPLFEKIDASIEKKANLFENSF
                     SRYAVRAVLACLFLTLGTAVAFAIAMKGEDIAHGLGKILYAFMFSWGLVMILYMNAEL
                     GTSNMLYMTVGVFRQRLALSKAAKILFACIFFNMIGGILCGFLISMTTPFQDLTLDNF
                     MLESIAVKLAKPSATILVEGMFANIVVNTAVLISMRMKEDVGKVITVVFIIFIFAFLG
                     YEHVIANFPAFTLTYFASHGQMDGMTVSNVLHNLFFALAGNYIGGGLVMGLGYAWLNQ
                     SKSSYVD"
     gene            8197..8832
                     /locus_tag="U2K71_001730"
     CDS             8197..8832
                     /locus_tag="U2K71_001730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HEM7315810.1"
                     /translation="MEVLVFPKNEEKALQMEKYMKNLFSFAGVPKPERALLQQPLLDE
                     SKNLSISEVLAFVHYYYEQIEREYQYVAIDLVQKNIKRLTFEDLVVLLPLVQKKEWWD
                     SIDSWRKVYSTWCKAYPEQREEVFQLFEKKEDFWLRRIAITLQLGYKQQTDTGLLIRA
                     IDEDRQTKEFFIQKAIGWALRDYSKTDADWVKRQLTTDLSKLAVREASKYL"
     gene            8852..9625
                     /locus_tag="U2K71_001731"
     CDS             8852..9625
                     /locus_tag="U2K71_001731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM7315811.1"
                     /translation="MRRKLFAFDIDGTLLDSNKQALDSTREALEKLRKAGHLVTIATG
                     RSRFHAQEIIRDLDFTNYILCNGAAGFLDHEQVYKNLLDRDQLDAFVQEAHHEQIDTA
                     FVSLDNIKRSSSNDVTVMEEAMHSFGAVLPELDQFFAEEQEIYQALAFFDQTYDGKFS
                     SYDKIRFVRWHENSVDVVPKNGSKAATILHLAEQVGIPHEDIISFGDGQNDREMLRMS
                     GIGIAMGNAVPEVQAEAKMVTDTNDQDGIWKALKELSVI"
     gene            10033..10830
                     /locus_tag="U2K71_001732"
     CDS             10033..10830
                     /locus_tag="U2K71_001732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311354.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HEM7315812.1"
                     /translation="MRVLFIGDVVGSMGREMITEYLPRLKKKYRPQVTIVNGENAASG
                     RGITEKIYKKFLQDGVDVVTMGNHTWDNRGIFEFVNEAKKMVRPANFPEGTPGQGMVF
                     VKVNQVELAVINMQARSFMVDLDDPFRKMNELVEEARKRTPIIFVDFHGETTSEKQAM
                     GWFLDGKVSAVVGTHTHVQTNDARILPRGTAYLTDVGMTGPYDGILGMRREPVIEKFL
                     TALPKRFEVVEQGRSILSGCILELDDTTGHAKEIQLIQINDDRPFME"
     gene            10808..11221
                     /locus_tag="U2K71_001733"
     CDS             10808..11221
                     /locus_tag="U2K71_001733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YlbF family regulator"
                     /protein_id="HEM7315813.1"
                     /translation="MIDRSWSRKRGSFLQQEPEIQKEVRKLTKLLRENETIIRYKELE
                     EKIQQNQYLAELREKIKQAQKDAVHFAHYDKPAAEKEAIKQADQFMQEFDQHPLVVAY
                     RKQLLEADDLLHHLTTMIQEEINGQIEEEKHASKN"
     gene            11205..13850
                     /gene="mutS"
                     /locus_tag="U2K71_001734"
     CDS             11205..13850
                     /gene="mutS"
                     /locus_tag="U2K71_001734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HEM7315814.1"
                     /translation="MPQKTKNTPMMEQYLSIKAQYQDAFLFYRLGDFYELFYEDAIKA
                     SQLLELTLTSRNRNAEEPIPMCGVPHHAAQGYIDALIEKGYKVAICEQVEDPKTTKGM
                     VKREVIQLVTPGTVMDSKGLSAKDNNFLTAVVSQGNEFGFAYADLSTGELKTARLHDE
                     EAVLNEASALQTKELVLGSEITDTLREQLSGRLGLVFSQQNEMEENAEFSFLTSELVD
                     PLEKEATGKLLTYLSVTQKRSLAHIQKAVEYQPDHFLKMDYYSKFNLELSQSIRTGKK
                     HGTLLWLLDETKTAMGGRLLKQWLDRPLIQAKQIKARQEMVASLLDSYFERIDLQSAL
                     TKVYDLERLAGRVAFGNVNGRDLIQLKTSLEQVPMIRGLIEGIDQGQWQSLLSEMQPM
                     DHLVQLIDQAIQDDPPLQITEGNIIKDGFNEQLDMYRSAMRNGKKWLAELEAKERQET
                     GIKNLKIGFNRVFGYYIEITKANLGNAELEKYERKQTLANAERFITPELKELETQILE
                     AEEKSVDLEYQLFLAVREEVKKAIQPLQVLAKAISAVDVLQSFATISERYQYVRPELV
                     SDKHQLLIKDGRHPVVEKVLGHQEYIPNSVEMAEDEMILLITGPNMSGKSTYMRQLAL
                     TVLMAQMGCFVPAKQAVLPIFDRIFTRIGASDDLIAGQSTFMVEMMEANQALRYATPN
                     SLILFDELGRGTATYDGMALAQAIIEYIHRHVQAKTLFSTHYHELTVLEKELPQLKNV
                     HVGAVEKDGEVVFLHKMMDGPADKSYGIHVAKIAGLPSNLLERAADILHTLENGDNGH
                     HTVPAEVEEKTKTAKQEQIKPPAEKVNQDHIQDRVKEENQQLSLFSELSENETEVIES
                     LKQINLLEMTPMDALNTLYELQKQL"
     gene            13868..15976
                     /gene="mutL"
                     /locus_tag="U2K71_001735"
     CDS             13868..15976
                     /gene="mutL"
                     /locus_tag="U2K71_001735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HEM7315815.1"
                     /translation="MAKIQELSERLANQIAAGEVVERPASVVKELVENAIDAGSTQID
                     ILVEEAGLRTIQVIDNGEGILADDVENAFKRHATSKIHNRDDLFRIRTLGFRGEALPS
                     IASVSEMTLETANQEEKQGTYLSLKGGEVIEHRPAPLRQGTKITVSNLFFNTPARLKY
                     VKTLHTELANIGDIVNRLALSHPSIAFRLVHDGNKMLATAGNGDLKQTIAGIYGLSTA
                     KKMLKIEGKDLDFELFGYVSLPEVTRASRNYLSTIINGRFIKNFSLNKAIVAGYGSKL
                     MVGRFPIAVLEIKMDPLLVDVNVHPTKQEVRLSKEKELTELISNAIQKALSQENLIPS
                     AADNLRFKKKVPDQPKTEQIEIDLSYAFEEEKPKKPSSLNFDRQTGTFFVEELKNTFV
                     DRIVDKSVENVENYSGFSEENTTYPEKTAKTVDKFGENVDNFVEKTVEKSEKKEFPIV
                     DNMEKTNEEQADKSVEEDEKIYEEELTLHPEFDFTQGNDKTLEQAIKKLETEKPVQRF
                     PQLEYFGQMHGTYLFAQSSDGLYIIDQHAAQERIKYEYFREKIGEVSDDLQELLVPFV
                     LDYPNSDALKLKEQKHLLEDAGIYLEEFGQNSFIVRAHPTWYPNGEEESIIREMIDML
                     LTTGSVSVKKFREATAIMMSCKRSIKANHYLNEAQARVLLKDLARCENPFNCPHGRPV
                     LIHFTNSDMERMFKRIQDPH"
     gene            15998..16558
                     /gene="maf"
                     /locus_tag="U2K71_001736"
     CDS             15998..16558
                     /gene="maf"
                     /locus_tag="U2K71_001736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation inhibitor Maf"
                     /protein_id="HEM7315816.1"
                     /translation="MKIVLASQSPRRKELLAHIVSEFEIEPADIDETPFPDEQPVDYV
                     RRMAEEKARTVWEQDEKEDHLVIASDTTVVLNQEIMGKPEDLAEAEFMLKKLSGETHT
                     VYTAVVLKTKEREERILAEAHVTFYPLTDEEIKRYLETGDYADKAGAYGIQNAASVFV
                     KEISGDYYSIVGFPIGAVNQALKTFI"
     gene            complement(16643..18631)
                     /gene="treP"
                     /locus_tag="U2K71_001737"
     CDS             complement(16643..18631)
                     /gene="treP"
                     /locus_tag="U2K71_001737"
                     /EC_number="2.7.1.201"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system trehalose-specific EIIBC component"
                     /protein_id="HEM7315817.1"
                     /translation="MAKYQADAEKLLHDIGGKENIAAVSHCATRMRFVLNDPGKADEK
                     AIEDIPSVKGMFTNAGQFQVIIGNDVSTFYNDFVAVSGVEGVSKEQGKAAAKQNLHPV
                     QRAIAVLAEIFTPLIPAIIVGGLILGFRNVLEGIQFESLGGTIVEHSKFWNGVNAFLW
                     LPGEAIFHFLPVGITWSIAKKMGTTQILGIVLGITLVSPQLLNAYSVASTAAADIPFW
                     DFGFAQVQMIGYQAQVIPAMLAGFMLAYLEIFFRKYIPQSISMIFVPLFSLLPTVLAA
                     HVILGPIGWTVGSWISNIVNTGLTSSISWLFSAVFGFLYAPLVITGLHHMTNAIDMQL
                     IADFGSTNLWPMIALSNIAQGSAVLAIIFLHRGNKKEEQISIPAMISCYLGVTEPAMF
                     GINLKYVYPFVAAMVGSGLAGMFANLMGVRANAIGVGGLPGILAIQAETWVPFIIAMI
                     IAVIIPFGLTIIFRRQGILNKIDPAVPENAADVQLQTANGATATPQSFEPVSATGTAV
                     ATKETLFAVAAGTIKEITEVNDPVFSQKMMGDGYAVEPSNGKVYAPVNGKVTSVFETK
                     HAIGILSNEGLEVLVHMGLDTVELKGVPFNVFVKEGYLVTPETLIAEMDLPEIEQAGK
                     KTDIIVALTNNEKVAGLSLDQSGLVRPGEAVGKAEVKS"
     gene            18854..19567
                     /gene="treR"
                     /locus_tag="U2K71_001738"
     CDS             18854..19567
                     /gene="treR"
                     /locus_tag="U2K71_001738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose operon repressor"
                     /protein_id="HEM7315818.1"
                     /translation="MNKFNEIFLDLEQKILAKEYPPHSLLPSENQLIKIYGVSRETIR
                     KALNLLTTEGYIQKKQGKGSIVLDRNRFDFPISGLTSYKELQETQRIPSETIIHTLEE
                     TEVTKALNEITGWEIGAPVWHLIRERKINGEVVILDTDYLLKEIVPILTPAQAQGSIY
                     EYFENELSLTIAYAQKEITVEEVTEIMKTAMDLHDDTYAVVVRSLVHLEDTRCFEYSE
                     SVHRLDKFRFVEFARRRKV"
     gene            19644..20090
                     /gene="msrB"
                     /locus_tag="U2K71_001739"
     CDS             19644..20090
                     /gene="msrB"
                     /locus_tag="U2K71_001739"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HEM7315819.1"
                     /translation="MDKPIRPEKEELKEKLSPMAYAVTQENATERPFSGKYDDFYEKG
                     IYVDIVSGEPLFSSAEKYDAGCGWPSFSKPITKRQVREKADFSHGMHRIEVRSKEADS
                     HLGHVFTDGPVDQGGLRYCINSASLKFIPYEKMDELGYGEFKSLVE"
     gene            20260..20862
                     /gene="ruvA"
                     /locus_tag="U2K71_001740"
     CDS             20260..20862
                     /gene="ruvA"
                     /locus_tag="U2K71_001740"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HEM7315820.1"
                     /translation="MYEYLTGVVTFINPYYIVVETNGIGYQIALGNPYRYSSKLNQKI
                     KIYVHQVIREDAHLLYGFDSLEEKQLFLRLVSVSGIGPKSALAIMASEDHSGLIQAVE
                     SSDVTYLTKFPGVGKKTAQQMILDLKGKFGELSIDTPFSLFDEANTKDATALSEAMEA
                     LSALGYSDREIKRVEKQLKEVDNQTTDEYLRQALKLMMKK"
     gene            20875..21876
                     /gene="ruvB"
                     /locus_tag="U2K71_001741"
     CDS             20875..21876
                     /gene="ruvB"
                     /locus_tag="U2K71_001741"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HEM7315821.1"
                     /translation="MTDEYLLSPESGENELSLEKSLRPQLLSQYIGQRKVKQELQIYI
                     EAARNREEPLDHVLLYGPPGLGKTTMAMVIANEMTVNIRTTSGPAIERPGDLVAILNE
                     LEAGDVLFIDEIHRLPRVVEEMLYSAMEDFYVDIMVGQGPTAHPVHFPLPPFTLIGAT
                     TRAGMLSAPLRDRFGIISHMEYYDEQDLQEIVLRSSDIFQTEIIEEGALEIARRSRGT
                     PRIANRLLKRVRDFAQVQGDGKIDRPIADQALTLLQVDQAGLDYVDQKLLRTMIELYG
                     GGPVGLSTLSVNIGEERETVEDMYEPYLIQKGFLKRTPRGRIATAYAYEHFGYDYHI"
     gene            21905..22483
                     /locus_tag="U2K71_001742"
     CDS             21905..22483
                     /locus_tag="U2K71_001742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7315822.1"
                     /translation="MSDTTRTKQLIKNVLIDLCQTQSFRKISVQKIANEAKINRQTFY
                     YHFTDKYDLLRWVYCEDALHYLDPQCLSLDNWEEQALKMLKAIAEKKEFYANTVLSDP
                     EILQKEFSQLIQKSFIKIFEQMDEERQLSARDKLFYARFFSYGCSGVLVNWITNEYEE
                     TPMEIAMQLFRLAKDTELYSYRLYAKEELEEQ"
     gene            22535..23017
                     /locus_tag="U2K71_001743"
     CDS             22535..23017
                     /locus_tag="U2K71_001743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="low molecular weight phosphotyrosine protein
                     phosphatase"
                     /protein_id="HEM7315823.1"
                     /translation="MTKILFVCLGNICRSPMAEGLLRKRIALEGREAEFFVDSAATST
                     YEVGKTPHPGTKKILNQEKVDMTNMVARQITPHDFETFDWIIGMDQENVEELKRRAPR
                     SAQGKIHLFLSSIPGKEKENVPDPYYTNNFDETYHLITEGLNHWWNIWIDENKITNNL
                     "
     gene            23134..23508
                     /locus_tag="U2K71_001744"
     CDS             23134..23508
                     /locus_tag="U2K71_001744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315824.1"
                     /translation="MNEETVSPMKKLTKLVTHTSYQLFDSFVNDTEEHAKNVRLQGKF
                     ILELQKAATEHSLVILQVRANRLSSKYETISGWVATKTVEKDRVMIRLQNEEQQIRMV
                     PVEQIEKITILSPAGEQERLSK"
BASE COUNT         7781 a         4286 c         5115 g         6645 t
ORIGIN      
        1 aaaaaaactt ggtacgcaat cgctgtacca agttttttta tttagctttt gattaagcca
       61 taaataataa tttatataag aatactgcta cgacaccagc acagataggt gctacaactg
      121 gtacccatgc atatccccat tgagaagaac ctttatgttt caatggaaga agtgcgtgta
      181 caagacgtgg agcaaagtcg cgggctgggt ttagtccagg acctgtagga ccaccaagtg
      241 aagcaactaa tgtccataca aggaaaccaa gtgctaagtg agctgtacca agattatctt
      301 ggaagaatgg tgcttttgta atacctaggg caccaaagaa taaaacgaat gaaccaaaga
      361 attcattgac gaaaccattg aatttagaac caacgctgtt gattgttgag aatgtaccta
      421 acacatggtt tacgtcttct gtttggtcgt agtaaggttt gtaacaagct actacgatca
      481 attgtccaat cgctgcacca atcaattgtg caatgatata aggaacaact tcagcccaag
      541 ggaatagacc attagatgct aaagcaattg taaatgctgg gttgatgtgg ttaccgctga
      601 ttccaccaaa aatcattgct ggcatcataa ccccgaatcc atacccgaca gcaatcagca
      661 tccagtcact gccatatcct tttgtacctt ttaaatctac gtttgctaca gcaccgttac
      721 ctagaacgat tagtaatgct gtaccaatca tttccgccgc aagacgggtc attagtgagt
      781 aatccataaa tacctccaaa agttataaaa atgcaaaaaa aaaagaaaca tcaacacgaa
      841 aagcatcggt gcagtgtttc gtgtcggaag gatttctgca gtcttaacaa atgtgaataa
      901 aaaaatgaat tccattactt ataagaaacc agaccaacag taaaactcca actcttttta
      961 aaagttctcc acaaatcacc aagcttaact ataaatatac caaaaaaaaa acgttttttt
     1021 tctcaaaaat tgtatcttag aaacagcatg ttttgaaact ttcagaaaag gtttgttagt
     1081 aaaatgaaag ctatacgtaa ggagtgttgt ttgatgaaat tttttcaaga gtttttcatt
     1141 attattttct tttctgtatt aggagaaggc atacggattt ttgctcattt acctattcct
     1201 ggaagtatct taggtattct ttttttattt ttagcatttg aaatgcatct tgttgatcct
     1261 gaaaaaatcg gagcaactgg tgatttttta ttgaataatc tagccatttt atttgttcct
     1321 gctggtgtag gtttattgga atattttgat gatattgctt cgatatggcc tgttttactg
     1381 ggagcagtcg tcgtgtgttc catcgtcacg atggttgcag cgggaaaaac ggcagaaggt
     1441 gtagaagctt tgctgggcta tgtccgtaag aaaaaagcag aaagggtatc tgtaaacgaa
     1501 atggaggaga atcaaattga ctgagttttt tgtgaatcca ttatttggct taagtttgac
     1561 tttaggagtg tacgtattat ctgacaaatt tttgcggaaa tataaactgc ctgttttgaa
     1621 tcctctcgta ttatcgattg ttgttattat cttatttttg catttttctg ggatcagcta
     1681 taaagactat aatatcgggg gcaatttttt gactatgctg attactcctg cgacagtggc
     1741 acttgccatc cctctttata aaaacttccg tttactgaaa gagaattttt atcctgtaat
     1801 ggctgcgatt ttagcaggaa tagtcgcaaa ttgcttggtt agtgtagggt tcggttactt
     1861 cttctctctt cataaagaga tggtgatctc attactacct aaatctgtaa cgacggctat
     1921 ttctgtcgat ttatctcata cgatgggcgg ggtgaatgcc gtgacacttg ctattgttgt
     1981 ctctacaggt atttttggtt cgctgatcgc cactcatatt ttccgcctat tcaagatcga
     2041 aagtccagtt gctcgcggtg tggcgttagg aagtacttct catgctattg gtacagcaaa
     2101 agcaatcgag attggtgaaa tagaaggaat catttccgga cttgcgatat gcgtcaacgg
     2161 aatattgacc gttttattgc taccgttatt ctttcagcct tttgctggct tgttttgatg
     2221 cgaaaaccac tattttatgc tacaataacc aaaacgttcg agcataaaaa aggagggaaa
     2281 gacgtgtatt tgtcttccga gtatcttaaa caatttgata aagatctata ttataagatt
     2341 ctcctcctag aaagttttga ggatcaagct tggcataccg ctgcacaatt agcgcaagtt
     2401 gttcagctgg atgcccgcag cgtgtcgaaa tacctgaacg agctgtctaa aaactatcag
     2461 cagtttagcg gtaagacaca ccctttgttt acaaaaaatc accgttctgg ttataacttt
     2521 tatgatacgc tcgattcaat tgaacatgag cgttttttga tctatctcgt gcaaagtacg
     2581 ctgaaattcc agttgctgca cgacattttt tttgaagaat tccatacaat gtaccaattt
     2641 gcgcaaaagc attacattag tgaatctact gctcatcgaa aaatcaatga atggaaacag
     2701 caactgcaaa cttacggaat tcggctgcaa cgaggtacat atatcgctca aggcgaagaa
     2761 gaaatcattc gactttactt gcatatgaca ttctggcagt tgtttcgagg gaaaatctgg
     2821 ccatttgaaa cgatttctca aatggacgta aagaatatgg cagaacatat catggctttt
     2881 tttaatgttc gtctgaacga gatcaaaaaa agacgattgg aatatatgct aggtgccttt
     2941 tttttaagaa aatcacagaa gcattatgtt gtattgaatg agaaaaaaag acggctgatt
     3001 tctgacaatc tgttgttcca gcgattttgc caggtcatgg agccggtatt tcctaattat
     3061 tttcaagtag aagacgagtt aggcgcatta tttcttgtac tgatgactcg tgaagaatat
     3121 tatagtgatc ctaagatacg gaagaaaatt tttgattttc atcaacaagc aaaaacaccg
     3181 ccctttacag ccctttcaga agcaaaagcc gctctttctc tttatcagga agagcagggg
     3241 ctgccagcag aaaatctaac cttcgaggca gaaaactatt tattcagcag tcattttttc
     3301 gcctatcttt ttcctaatgc gaaagaaacg attgatggca atagtagtga ttttattaat
     3361 catttagtta ttgaaaacaa ggaattgaaa caatggctcg tacatttttt tgaaagtaga
     3421 cacaaacatc ccaatcatct ggcatttaaa aatcatgcgt ttttgatggg gcggtatctg
     3481 actgtgttta aaacattagg agcctttact ccacagcttc caaagatcac gatcctttta
     3541 atgacagatt tcccattatt tgaagagcaa ctgttagagg agggattacg gacatttttc
     3601 cgtaatgagt atcagctgat ttttctgcca acagattatc gggggagaga agttgatctt
     3661 ctgatttcga caagtaaagt ccaccgcaaa ccatgggcgg atcttgacta ttttattgtg
     3721 actgaagaac taaaattaat tgattacatc caactatctc aaaaatttga aatgattcaa
     3781 aaacaaaaac tattaaaaca ataacaaaga aggagaaaaa agatgacatt tgaagaaatt
     3841 ttaccagaat tgaaaaaagg tgcaaagatt attcgtaaag gatggagtgg atttgaactt
     3901 tatgttgttc tcgtatccga tgatgaattt gatggctgtc cggtgacgcc gtacttattg
     3961 atcaaaacaa gtgacgaagg tttttccagc ttcgctccta cagtttgtga tattttagca
     4021 gatgattggg cgatcgtaga atgaaaaacc agtatccaga tctagctaaa caggttgtat
     4081 ttatcacagg agctgcttct ggaattggtg ctgcgcaagc ccgagcgttt cttgaacaag
     4141 atgcttttgt atttggatta gatcaaaagt tcggtaaaat ggaagaaatt tctttgcgat
     4201 ttcctgaaac ttttgcctac gcactaggtg atgttcgaca aatgaaagaa ctccaacaag
     4261 cggtatcgaa atgccaaaat cattttggtg aagttacgat tttactgaat acagcgggga
     4321 tattggatgc ctacaaaccc ttgatggaaa ctgacgaatc gctgtgggat ctgatttatg
     4381 agacgaatgt gaaaagcatg tatcagctga caaaaatcat ccttcctgat atgatccgcc
     4441 aaaaaagcgg aacgatcatc aatatggctt cgattgctgg cttgattgct ggtggaggtg
     4501 ggatcgctta tacaagcgcg aaacatgcga ttgtcggatt taccaaacaa ctcgctctgg
     4561 attacgcagg acagggtatc cgagtaaaag gaattgctcc aggagccatc caaacaccaa
     4621 tgaatgcggc tgattttgaa gggaaaggtg agatggcaga gtgggtggca aatgaaacac
     4681 cagtcaagcg atgggcacag ccggaagaag tagctgagct gacgttattt cttgcctctc
     4741 ctcaagcaag ctacatacaa ggaacgatca tcccaatcga cggcggatgg ttgttaaaat
     4801 aaccttgtgt ttttttcaag cttctgtatg ataaaactaa agagagaaac aacggttgat
     4861 gataaaaaaa gtcgatgact gttcccgaca ttcgatgccg aacagccagt tcaaaaaatt
     4921 ctttttaata atcgttaata ttcgaaacta agcggctttt cacgacctct tgatataaac
     4981 gatattcaaa agcttgcaac tgcggtaaca agtccagcca aatgaaaaat ataaggagct
     5041 atttttattt gaccattctt attacttgtt gcagggcagc tttttcacta ccttctgcga
     5101 cacttcagtt ggaattttct tcccatcctt tggtcgcggt cattaccaag ataaaattat
     5161 cttgcaaagg agaaatagaa catggaaaaa aataataccc gttcagccaa acgctggaca
     5221 acgacagatg tgactcggat ggcgctagtc actggtttat atgtggcagt gacgctggtt
     5281 ttgtctgtct ttagctttgg agtgattcag attcgtctct cggagatgtt caactatctg
     5341 tccttgtata acaaaagata catcgtcgcg gtaacagcgg gagtagcttt ggctaatatt
     5401 gcttctcctc ttggactgat agatgttgca gtaggaagcg tcagcacgct gcttgtattg
     5461 cttatcaact ataaaatcac tagtcgcatc aaaaacatga aaatcaaaat ggccgtgaca
     5521 gcatgcgtat ttgctttttc catgtttaca gtagctggtc agctaacgat tttatatcaa
     5581 ctaccttttt tcttgaattg gtgggtgatt gctttaggag aattggcatc aatgattgtc
     5641 ggtgggataa ttatttattg gatcggtaaa aaagtggatt tgtcaaaata aagatctgtt
     5701 taatttaaaa tagggcagca tggaagagac tgaatgttga gtcttcttcc atgctgcttt
     5761 tttgattaat ttgtatgaag aaatcgattt agccgttttt tcatcaagat accgatataa
     5821 gctgctccag ttgccttgat tgcttccggc agtaaaaacg gcagtagtcc actggcaaat
     5881 gcagttgtaa aagtaagatc accacttatt ttcagccaga tcgttccgca gatcaatgca
     5941 gcaagagagc ccattagatt ggcaaagatt gcatataagt aatgagcatc cgttctttcg
     6001 atcatccaac cagttatcca cgtttgtaag agaaatccaa tcaaatatcc gccagttggc
     6061 ccaaataaaa cagctatacc tccagtcatc cctgaaaata caggaagacc aatcaagcca
     6121 agtaaaagat aaactccaac agatatcaat cctagttttc ttccgagaaa agttgctgtt
     6181 aatccaatag caaatgtttg ccctgtcaaa ggaaccaaac caaaaggaat ggtcatctga
     6241 gaaaggacag aaataatcgc ggcaaattcc gcagcaagta tcaattcttt caatgttcgg
     6301 ttcataacgc cctcttttcg ttaacttaat atttttattt ggttaacgaa attgtaaaac
     6361 aaagtgatta ctttgtaaat agaaaatata aaaaaagacc agctatccaa tagatagcgg
     6421 tctttttcaa gaaagctttt gaaattataa gctttggttg tagtattcaa cgacaagagc
     6481 ttcgtcgatt tctggatata gttcgtcacg ttctggtaaa cgagtcaatg aaccttccaa
     6541 tttttcagcg tcaaagctta cgaatgctgg gcgtccaaca gttgcttcta cagcttcttt
     6601 tactgttacg atgttttgtg atttttcacg aactgagatc acttgaccca cttctacgtg
     6661 gtatgaaggg atatcaacgc gtttgccatc aactaaaatg tgaccatggt taactaattg
     6721 acgtgcttga cgacgagtcg ttgcaagtcc taaacggtaa acaacattgt ctaaacgttg
     6781 ttctagtaag atcatgaagt taacaccatg tttaccttct ttgattttgc tagcttttac
     6841 gaacaagtta cggaattgac gttcgttcat tccgtacatg tgacgaagtt tttgtttttc
     6901 agttaattgc atgccgtatt ctgattgttt gccacggcta ttaggtccat gttgacctgg
     6961 tgcgtaaggg cgacgtgcta attcttttcc tgtgcctgac aatgagatgc ctaagcgacg
     7021 agaaattttc catgatggtc ctgtataacg agacattaaa atttcctcca atgaatataa
     7081 gtttttggag taaaataatc cgttgaaaaa ttcataatca tatagtttgt tcttcaatct
     7141 tcacctttgc agccgcggtt acacaatcga acccgagacg gggcaacaaa ctagggatga
     7201 gttattattt ttctgctgca ttattttaca caaattacag tatatgatag atcatccacg
     7261 aatgtcaaga taattagtac ataaaaacgc tttacaaacc taggctgtct ttataaaatg
     7321 agagggtatg tgcaaatgga aaaaagcgaa ggaagtttaa ggagaaaatc aatgaatccg
     7381 gtatcaccat tatttgagaa aatcgatgct tcaattgaaa agaaggcgaa tctttttgaa
     7441 aacagctttt cacgttacgc tgttcgagcg gttctcgcct gtttgttttt gactttagga
     7501 acggctgtgg catttgcgat cgcgatgaag ggggaagata tcgcgcatgg tctaggaaag
     7561 attttatacg catttatgtt cagctggggt ctggtcatga ttctatacat gaacgccgaa
     7621 ttaggaacat cgaatatgct ttatatgacc gtgggtgttt ttcggcagag acttgcttta
     7681 agtaaggcag ccaagattct atttgcctgt atttttttca atatgattgg cggtatatta
     7741 tgtggatttc taatttctat gactacgcca tttcaagatt taacgttgga caactttatg
     7801 ttagaatcaa tagcagtaaa actagcaaaa ccttcagcaa cgattttggt ggaagggatg
     7861 tttgcgaata tcgttgtcaa taccgctgta ctgatcagca tgcggatgaa agaggatgtc
     7921 ggaaaagtga tcacagtcgt attcatcatt ttcatttttg cttttttagg atatgaacac
     7981 gtgatagcga attttcctgc ctttacgctt acttactttg cttctcacgg acagatggat
     8041 ggaatgacag tcagcaatgt gttacataat ttgttttttg ctttggcagg aaattatatt
     8101 ggtggcggtt tagtaatggg actgggatat gcttggctga atcagtcaaa atcatcctat
     8161 gtggactaaa aagtcgttca ttaaagggga agtgccatgg aagttttagt ttttccaaaa
     8221 aatgaggaaa aagcgttaca aatggaaaag tacatgaaaa atcttttttc ctttgctgga
     8281 gtacctaagc cggaacgcgc tcttttacaa caaccgttac tggatgagag caaaaattta
     8341 tctatttctg aagttttagc ttttgttcat tattattatg aacaaatcga aagagagtat
     8401 cagtatgtag cgatcgattt agtccagaaa aacatcaaac ggcttacatt tgaagactta
     8461 gtcgtattgt tgccactagt tcagaaaaaa gaatggtggg acagcatcga ctcttggaga
     8521 aaagtataca gcacatggtg taaagcctac ccagagcagc gtgaagaagt ctttcaattg
     8581 tttgaaaaaa aggaagattt ttggctacgt cgaatagcaa tcacgttgca attaggttat
     8641 aaacagcaaa cagacacagg actattgatc cgagcaatag acgaagatcg tcaaacaaag
     8701 gaattcttta tccaaaaagc aattggttgg gcattacgcg attatagtaa gaccgatgcc
     8761 gattgggtaa aaagacagct gacaacagat ttgagtaagt tagctgttcg agaggcaagt
     8821 aagtatttat aagaaagcga gacgacaaga gatgagacgc aaattatttg cttttgatat
     8881 tgatggaacg ttgttagatt caaacaaaca agctttagat agcacaagag aagcattgga
     8941 aaaattacgc aaagccggtc atctggtcac gattgctaca gggcggagcc gatttcatgc
     9001 gcaagagatc attcgcgatc ttgattttac gaactacatt ttatgtaatg gagcggccgg
     9061 ttttttagac catgaacaag tatataagaa tttactagat cgcgaccagt tggatgcgtt
     9121 cgttcaagaa gcgcatcatg agcagatcga tacagctttt gtcagcttag acaatatcaa
     9181 gcgttcttct tccaatgatg taacggtgat ggaagaagcg atgcattctt tcggtgctgt
     9241 cttgcctgaa ttagatcagt tttttgcaga ggaacaagaa atataccaag cgttagcatt
     9301 tttcgatcaa acatatgatg ggaagttctc ttcatatgat aaaattcgat ttgtccgctg
     9361 gcatgagaac agtgtagatg tcgtacctaa aaatggttct aaagcagcga caattctgca
     9421 cttagcagaa caagtaggca tccctcatga agacatcatc agctttggcg atggacaaaa
     9481 cgaccgtgaa atgttgcgca tgtcaggaat tgggatagct atggggaatg ctgtgccaga
     9541 agtacaagca gaagctaaga tggtcactga tacaaacgat caggatggaa tttggaaggc
     9601 attgaaagag ttaagcgtga tttaaaagag gttgtgaaaa agctgttctg catcaagtaa
     9661 taagcccagc caaacaaaaa tagctcctcc tatttttcgt ttttctgggc ttattaccgc
     9721 agatgcaagc tttcgaacac cgtttaagga agaggtcgtg acagaagtgg tcagtttcaa
     9781 gaaataagaa gggatttacg aaaattgctt ctcaaatttt tgtaaaatta cggcttattt
     9841 ctgaagaagt agatgaaaca acgtttattc gggaaatggg actgtgacaa gatgtttgtc
     9901 atagtcccat ttttttattt tatttttcaa aactaagaaa taccaggaaa gagttccttt
     9961 tgtttgacct tttctttctt attgaaaaca actataatat agagtgatgt taaaagagaa
    10021 gaggaattta gtgtgcgggt attgtttatt ggtgatgtag taggttcaat gggacgagag
    10081 atgattactg aatatcttcc tagattgaag aagaagtacc gtccgcaagt aacgattgta
    10141 aatggtgaga atgccgcttc tggacgtgga atcacagaaa aaatctataa aaaatttttg
    10201 caagacggcg tagatgtcgt gacgatgggg aatcatacgt gggacaaccg cggaatcttt
    10261 gagtttgtaa atgaagctaa aaagatggtt cgtccggcaa attttccgga agggacgcct
    10321 ggacaaggga tggtctttgt caaagtgaac caagtcgaac tggcagtgat caacatgcag
    10381 gctcgttctt ttatggtgga cttggatgat ccttttcgta aaatgaatga gctggtagaa
    10441 gaagccagaa aacgtacgcc gatcattttt gtagattttc atggagaaac aacaagtgaa
    10501 aaacaagcaa tgggctggtt tttagatggg aaagtttcag cagtggtcgg tacacataca
    10561 cacgttcaga caaatgatgc gcgaattttg ccaagaggaa cagcgtatct gacagacgtt
    10621 gggatgacag gtccctatga tggtattttg ggtatgcgca gagaacctgt aatcgaaaag
    10681 tttctgactg cattaccaaa acgttttgaa gttgtggaac aaggccgatc gattttaagc
    10741 ggctgtatct tggaattgga tgatacgaca ggtcatgcaa aagaaatcca gctgattcag
    10801 atcaacgatg atcgaccgtt catggagtag aaagagaggc agttttttgc agcaggagcc
    10861 ggaaattcaa aaagaagtaa ggaaactgac aaaactttta agagagaatg aaacgatcat
    10921 tcgctacaaa gaacttgaag aaaagatcca gcaaaaccag tatttagctg agttgagaga
    10981 aaaaatcaaa caagcgcaaa aagatgctgt tcattttgcc cactatgaca aaccagcagc
    11041 agaaaaagaa gcaatcaaac aagcggatca attcatgcaa gaatttgatc agcatccgct
    11101 tgttgtagct tatagaaaac agctgttgga agcagatgat ctgcttcatc atttaacaac
    11161 tatgatacaa gaagaaatca atggacaaat agaggaggaa aaacatgcct caaaaaacta
    11221 aaaatactcc tatgatggaa caatatctaa gcatcaaggc ccaataccaa gatgcttttt
    11281 tgttctatcg tttaggagac ttttatgaac tattttacga agatgcgatc aaagcgtcac
    11341 agcttttgga attaacgttg acgagcagaa atcgaaatgc agaagaacct attcctatgt
    11401 gcggtgtacc tcaccatgca gcacaaggat atatcgatgc cttgattgaa aaaggctata
    11461 aagtagcgat ttgcgagcag gtagaagatc caaagacgac taaagggatg gtcaagcgcg
    11521 aagtgatcca gctagtcact cctggtacag tcatggacag caaaggattg tcggcaaaag
    11581 acaacaattt tttaacagct gtagttagtc aaggaaatga atttggcttt gcttatgccg
    11641 atttaagtac gggagaattg aagacggctc ggttgcatga tgaagaagct gttttaaatg
    11701 aagcctctgc attgcagaca aaagaactcg tattaggcag tgagatcact gatacccttc
    11761 gtgaacagct aagcgggcgt ttagggctag tcttttctca acaaaatgag atggaagaaa
    11821 acgcggaatt tagctttttg acaagcgaac tagtcgatcc gctggaaaaa gaagcaaccg
    11881 gcaaactatt gacttattta tcggtgacac aaaagcggag tttagctcat atccaaaaag
    11941 cagtagaata ccaaccagat cattttttga aaatggatta ttattcaaaa ttcaacctag
    12001 aattaagcca atctattcgg actgggaaaa agcacggcac tcttctatgg ttgttagatg
    12061 aaacaaaaac agcaatgggt ggccgtttac taaaacaatg gctagaccgt ccgcttatcc
    12121 aggcaaaaca aattaaagca agacaagaaa tggtcgcttc tttgctagac agctattttg
    12181 aacgtatcga tttgcagtca gcattaacga aagtctatga tctggaacgc ctagcaggtc
    12241 gagtagcttt cggtaatgtc aatgggcgag acttgatcca gctgaaaact tctttagaac
    12301 aagtgccaat gatcagaggg ttgattgaag gaatcgatca aggtcaatgg caaagtctgc
    12361 tttcagaaat gcagccgatg gaccatctcg tccaattgat cgaccaagct atccaagatg
    12421 atccgccgtt acaaataacg gaaggaaaca tcatcaaaga tggattcaat gagcagctcg
    12481 atatgtaccg ttctgctatg agaaacggaa agaaatggtt ggcagaactt gaagcaaaag
    12541 agcgtcaaga aacaggaatc aagaacttaa aaatcggatt taaccgcgtt ttcggttatt
    12601 atatagaaat tacaaaagca aatcttggaa atgcagaact ggagaagtat gaacgaaaac
    12661 aaactttagc aaatgcagag cgtttcatca caccagaact aaaagaattg gagacacaaa
    12721 tcctcgaagc agaagaaaaa tctgttgatt tagaatatca gttgttttta gctgtgagag
    12781 aagaagtcaa aaaggctatc cagccgctac aagtacttgc aaaagcaatc agtgcagtag
    12841 atgtgctaca aagttttgca acgatcagcg aacgctatca gtatgttcgg ccagaacttg
    12901 tcagtgacaa gcatcagctt ctgatcaaag acggacgaca tcctgtagta gaaaaagtat
    12961 taggacatca ggaatatatc cctaacagcg tagaaatggc agaagacgaa atgatcttgc
    13021 tgattacagg accgaatatg tctggtaaaa gtacgtatat gcgacagttg gcattaactg
    13081 ttctgatggc gcaaatgggc tgtttcgttc cggcaaaaca agcagtttta cctatttttg
    13141 atcggatttt tacccgaatt ggtgcaagtg acgacttaat tgctggacaa agtactttta
    13201 tggtagagat gatggaagcc aatcaagccc tgcgttatgc tacgccaaac agtttgattt
    13261 tgtttgacga actagggcga ggaacagcaa cctatgacgg aatggcgttg gcccaagcaa
    13321 tcatcgaata tatccatcgt catgtacaag caaaaacact cttttctact cattatcatg
    13381 aactgacggt acttgagaaa gaacttccgc agcttaaaaa tgtccatgtt ggagccgttg
    13441 aaaaagacgg agaagttgtc ttcttgcata aaatgatgga tggacctgcg gataaaagct
    13501 acggcataca tgtggcaaag atcgcaggat tgccttccaa tctactagaa cgagcagcag
    13561 atatcctgca tacgctagaa aatggcgata acggacacca tactgttcca gctgaagtag
    13621 aagagaaaac taaaacagcc aaacaggagc aaataaaacc acctgcagaa aaagtaaatc
    13681 aagaccatat acaagaccgt gtaaaagaag aaaaccaaca gctttcttta ttcagtgaat
    13741 tatccgaaaa tgaaacagaa gtcattgaat cactgaagca aataaattta ctagaaatga
    13801 cgccaatgga tgcactaaac acattatatg aattacaaaa acaactgtag gaagaaggcg
    13861 gatatcgatg gccaaaatcc aagaattatc tgaacgttta gctaatcaga tcgctgcggg
    13921 agaagtagtg gagcgaccag cttccgttgt gaaagaatta gtggaaaatg caattgacgc
    13981 cggcagtact cagattgata tcttagttga agaagcggga ctgcggacga tccaagtgat
    14041 tgataacgga gaaggcattt tggcagacga cgtagagaat gccttcaagc ggcatgcgac
    14101 aagtaagatc cataatcgcg acgatctttt tcgcatacga acgctgggct ttcgagggga
    14161 agctttgcca agtattgcct ctgtttctga aatgactctg gaaacggcga atcaggaaga
    14221 aaaacaaggc acctatctgt cgctgaaagg cggagaagtt attgagcatc gaccagcacc
    14281 actgcgtcaa ggaacgaaga ttaccgtcag caatctgttt ttcaatacgc cagcccgctt
    14341 gaaatacgtg aaaacactgc acacagaatt agcgaacatc ggggacatcg tcaatcgact
    14401 ggcattgagt catccatcga ttgcttttcg tttagtacat gatggaaaca aaatgctggc
    14461 aactgctgga aatggcgatc tgaaacaaac aattgcggga atctacggat tatctaccgc
    14521 aaaaaaaatg ctgaaaatcg aaggaaaaga tctagatttt gagttatttg gctatgtttc
    14581 attgcctgag gtgactcgtg ccagtaggaa ttacttatcg acgatcatca atggacgttt
    14641 tatcaagaac ttttccttga ataaagcaat cgttgcaggt tatgggtcaa aattaatggt
    14701 cggtcgtttc cctattgctg tattggaaat caaaatggac ccgcttttag tagatgtaaa
    14761 tgttcatccg acgaagcagg aagtccggct gtcgaaggaa aaagaactaa cagagctaat
    14821 tagtaacgcc attcaaaaag cattgtctca agaaaatctg attccaagtg cagcggataa
    14881 tctacggttt aagaaaaaag tacctgatca gcctaaaaca gaacaaatcg aaatcgattt
    14941 gtcctacgct tttgaagaag aaaaacctaa aaagccaagt agtttgaatt ttgatcgaca
    15001 gacaggtacc tttttcgtag aagaactaaa gaacacattt gttgacagaa ttgttgataa
    15061 gtctgtggaa aatgttgaaa actattctgg tttttctgaa gaaaacacca cttatccaga
    15121 aaaaacagcg aaaacggtgg ataagtttgg tgaaaatgtt gataactttg tagaaaaaac
    15181 agtggaaaaa tctgaaaaga aagaatttcc cattgtggat aacatggaga aaacaaatga
    15241 agaacaagca gataaatccg tagaagagga tgaaaagatt tacgaggaag aattgacact
    15301 ccatcctgaa tttgatttca ctcaaggaaa cgacaaaaca ttagaacaag ccatcaagaa
    15361 attagaaacg gaaaaaccag tacaacgttt tcctcagttg gaatattttg gacagatgca
    15421 cggaacgtat ctttttgccc aaagtagcga tgggttgtac atcattgatc agcatgctgc
    15481 ccaagagcgc attaagtatg aatatttccg ggaaaaaatt ggggaagtat ctgatgatct
    15541 gcaagaattg cttgttccat ttgtgttaga ctatccaaac agtgatgctt tgaaattaaa
    15601 agagcaaaaa cacttgctag aagatgcagg gatttattta gaagaatttg gtcaaaacag
    15661 ctttattgta cgggcccatc ctacttggta tcccaatgga gaagaagaat cgattattcg
    15721 ggagatgatc gatatgctgc tgacaactgg aagtgtgagt gtaaaaaaat tccgtgaagc
    15781 aacagcaatc atgatgagtt gcaaacgatc gattaaagcc aaccattatt tgaatgaagc
    15841 acaagcacgt gtgctgctga aggatctggc acgatgcgaa aatcctttca attgtcccca
    15901 tggccgtcca gtattgattc actttaccaa ttcggatatg gaaaggatgt tcaaacgaat
    15961 ccaagacccg cattaagtca aagagaagag ggaaaacatg aagattgtat tagcatccca
    16021 atcaccaaga agaaaagagt tattggctca tatcgtttca gaattcgaga ttgaacctgc
    16081 tgatatcgat gaaacgccat ttccagacga acagcccgtc gattatgttc gtagaatggc
    16141 tgaagaaaaa gcacgaactg tctgggaaca agatgaaaaa gaagaccatt tagtgattgc
    16201 cagtgacacg actgttgttc tcaatcaaga gatcatgggt aaaccagaag atctggctga
    16261 ggctgaattc atgctgaaaa aactaagcgg ggaaacacat actgtatata cagctgttgt
    16321 tttaaaaaca aaggaacgag aagaacggat actagcagaa gcacatgtca ctttttatcc
    16381 tttgacagac gaagagatca aacgctactt ggagacgggg gattatgcgg ataaggcagg
    16441 ggcatacggc atccaaaatg ctgcgagtgt attcgtcaaa gaaatttctg gagattatta
    16501 cagtatcgta ggatttccga ttggtgctgt caatcaggca ctgaaaacat tcatttaaac
    16561 aaaaaagcgg actatctgat tttcgattga aaatccatga tagcccgttt tttttattga
    16621 aaagattact tatctactct gactaagatt ttacttcagc tttaccgact gcttcgcccg
    16681 ggcgaacaag tcctgattgg tcaagagaaa gtcctgcaac tttttcatta ttggtcaaag
    16741 caacaataat gtcagttttc ttgccagctt gttctatttc tggcaaatcc atttcagcaa
    16801 tcagggtctc tggtgtgaca aggtatcctt ctttcacgaa tacattgaaa ggaacaccct
    16861 ttagttcaac agtatctaat cccatgtgaa caagtacttc cagcccctca ttagaaagaa
    16921 taccaatggc atgttttgtt tcaaagacac tcgtcacttt accgtttact ggtgcataga
    16981 ctttgccgtt tgacggttcg acagcataac cgtcacccat cattttttgt gagaaaactg
    17041 ggtcatttac ttcagttatt tccttgatgg tccctgcagc gactgcgaat aatgtttctt
    17101 ttgttgcaac tgccgtacca gttgctgaaa caggttcgaa agactgtggc gttgctgttg
    17161 cgccatttgc tgtttgaagt tgtacatcag cagcattttc aggaacagca ggatcaatct
    17221 tattcaaaat tccctgacgt ctaaagatga tcgtcaagcc aaatggaatg atgacagcaa
    17281 tgatcattgc gatgatgaaa ggtacccatg tttctgcttg gatagctagt attcctggca
    17341 aaccgcctac tccgatcgcg ttagctcgaa cacccatcaa gttagcaaac atgccagcta
    17401 atccagagcc gaccattgca gcgacgaaag gatagacgta tttcaaattg attccgaaca
    17461 tagcaggttc agttacgcct aagtaacaag aaatcattgc tggaatcgag atttgttctt
    17521 cttttttatt cccacgatgt aagaagatga tggctaagac tgcagagcct tgggcgatat
    17581 ttgataaagc aatcatcggc cataaattcg ttgatccgaa atctgcaatc agctgcatat
    17641 cgatggcatt ggtcatatgg tggagaccag taatcactaa aggagcatac aggaatccga
    17701 atacggcact aaacaaccag ctgatagaag acgtcaatcc agtatttacg atatttgaga
    17761 tccagcttcc gactgtccag ccgataggac ctaaaataac gtgtgcagct aaaacagttg
    17821 gtaacaatga aaacaatgga acaaaaatca tcgagatcga ctgagggata tatttgcgga
    17881 agaagatttc tagatacgct aacataaacc ctgccagcat ggctggaata acttgcgctt
    17941 gataaccaat catctggact tgagcaaaac caaaatccca aaatggaata tctgctgcgg
    18001 ctgtggaagc tacactataa gcatttaata attgcggaga aactaacgta attcctaaaa
    18061 cgattcctaa tatttgtgta gtacccatct tttttgcaat actccaagtg atacctactg
    18121 gaagaaaatg gaaaattgct tcaccgggta accacaaaaa ggcattgaca ccattccaaa
    18181 acttcgagtg ttcaacgatt gttccaccaa gactttcaaa ctggatacct tcaagtacat
    18241 ttctgaatcc aaggatcaat ccaccgacaa tgattgcagg aatcagtggt gtaaagattt
    18301 ctgctaaaac ggcaatagca cgttgaacgg gatgaaggtt ttgttttgca gctgcttttc
    18361 cttgttcttt ggaaacccct tcaactccag atactgcaac gaagtcgtta taaaaagtag
    18421 aaacatcgtt tcctatgatg acttggaact gtcctgcatt cgtgaacatc ccttttacgc
    18481 taggtatatc ttcgatcgct ttttcgtccg cttttcctgg atcattcagg acaaaacgca
    18541 tacgagtcgc acaatgacta actgcagcaa tattttcttt tccgccgata tcatgtaaca
    18601 gcttctcggc atctgcttga tattttgcca tatcaataaa cccctttctc ttaacttgtc
    18661 tatgcaagtt gtttcttcat ttactatact ttttttcgga gagaattgca agcgttttat
    18721 taaaaagtac gagagaaaat gatatcgttt taaaaagctt aaaataagct tttcttaaaa
    18781 ataggaacat tgaaatacaa aaagagctgt agtatagtga agaaatgatg gaagaacgag
    18841 tgagggaaaa aacatgaata agttcaatga aattttttta gatttggaac agaaaatttt
    18901 agcgaaagaa tatcctccgc attcgctttt acccagtgag aaccaattga tcaaaattta
    18961 tggtgtttca cgagaaacaa ttagaaaagc gctgaatctt ttgactacgg aaggttatat
    19021 tcaaaaaaaa caaggaaaag gttcgatcgt actggatcgg aatcgcttcg actttcctat
    19081 ttctggattg actagctata aagagctaca ggagacccaa cgtattccaa gtgaaacgat
    19141 catccatacg ttagaagaaa cagaagtgac aaaagcattg aacgagatca ctggctggga
    19201 gatcggtgcg cctgtttggc atttgatacg cgaaagaaaa atcaatggcg aagtagttat
    19261 attagacacc gattatctat taaaggaaat cgttcctatt ttaactccag cacaagcaca
    19321 aggatcgatc tatgaatatt ttgaaaatga gctttctttg acgattgctt acgcgcaaaa
    19381 ggagatcaca gtagaagaag taacggaaat catgaaaaca gcgatggatc ttcacgatga
    19441 cacttatgca gtagtagtca gaagcctcgt tcatctggaa gatacccgct gtttcgagta
    19501 ttctgagtcg gttcaccgat tagataaatt ccgtttcgtt gagtttgccc gacgccgaaa
    19561 agtgtaaata gttcaaaaga aagcaaattg ttggcatctg tccactttgg cgctatgata
    19621 ggatcacaaa gggggcattt ttaatggata aaccaataag accagaaaaa gaagaactga
    19681 aagaaaagtt atcacccatg gcttatgcag tcacacaaga aaatgcaaca gaacgaccat
    19741 ttagcgggaa atatgatgat ttttacgaaa aggggatcta tgtagatatc gtcagcggtg
    19801 aaccgttatt cagctctgca gaaaaatatg atgcaggttg tggctggcca tcattcagca
    19861 aaccaataac gaaaagacaa gtgcgcgaaa aagccgattt ttctcatggc atgcatcgaa
    19921 tagaagttcg aagcaaagaa gcagattctc atctcggaca tgtgtttacc gatggaccag
    19981 tagatcaagg tggtctacgt tactgcatca attctgcctc actgaaattc attccatatg
    20041 aaaagatgga tgaattaggt tatggggaat tcaaatcact tgtagaataa tggaaataca
    20101 caaagagcgg tacaatacgg ggatttactc gatcgtccgc tcttttttta ttcaagcgat
    20161 tgctatttga aagaaacgct ccctagactc cttttttcac aaggtgaaat gtgctacaat
    20221 aaagggaacg tatgtgtgca aaagaaagag gactagaata tgtatgaata tctgacaggt
    20281 gtggttacct ttatcaaccc ctattacata gtagtagaaa ccaatggtat cggctaccag
    20341 atcgcactag gaaatcctta ccgctacagt agcaaactaa atcaaaaaat aaaaatttat
    20401 gtccatcagg taatacgtga agatgcgcat cttctgtatg gatttgattc gctagaagaa
    20461 aagcaactgt ttttgcgatt agtaagtgtt tcaggaatcg gtccgaaaag tgcccttgca
    20521 atcatggcaa gcgaagacca ctccggtttg attcaagcag tagaatccag tgatgtcact
    20581 tatttgacga aatttccagg agtagggaaa aagacagcac aacaaatgat cttggatcta
    20641 aaaggaaaat tcggtgagct gagtatcgat acacctttta gtctatttga tgaagcaaac
    20701 acaaaagacg ctacagctct atctgaagca atggaagctc tctcggctct tggttacagt
    20761 gatcgagaaa tcaaacgtgt agaaaaacaa ttaaaagaag tagataatca gacaacggat
    20821 gagtatctac gccaagcact caagctaatg atgaaaaaat aaggaggaga ccaaatgaca
    20881 gatgaatact tgttatcacc agaatctggc gaaaatgaac tgagtttgga aaaatctctt
    20941 cgaccacagc tgctttctca atatatcgga cagagaaaag tcaaacaaga acttcagatt
    21001 tacatcgaag ctgccagaaa tcgggaagaa cctttagatc atgttctgtt gtacgggcct
    21061 cctggattag gaaaaacgac gatggccatg gtcattgcta atgagatgac cgtaaatatt
    21121 cgtacgacga gtggccctgc tatcgaacgt ccgggtgact tagtagcaat cttgaatgaa
    21181 ctggaagcag gggatgtcct gtttatcgat gagatccatc gattacctag agtggtagaa
    21241 gagatgcttt attcagctat ggaagatttc tatgtagata tcatggtagg acaagggcca
    21301 accgcacatc cggttcattt ccctcttcct ccctttactt tgattggcgc gacgacacgg
    21361 gcaggtatgc tttctgcacc tttacgagat cgatttggca tcatttctca tatggaatat
    21421 tatgacgaac aggatctgca ggagatcgtt ttacgttctt cggatatttt tcaaacagag
    21481 atcattgaag aaggggcatt ggaaattgct cgccgatcac gaggaacgcc tagaatcgct
    21541 aatcgcttgc tcaaacgggt tcgtgatttt gcgcaagtcc aaggagacgg gaaaatcgat
    21601 cgtcctatag cggatcaggc acttactttg ctacaggtcg atcaggctgg attggattat
    21661 gttgaccaga aattgttgcg aacaatgatc gagttatatg gcggcggacc agtagggtta
    21721 agtacgcttt cagtaaacat aggagaagag cgtgagacgg tagaagatat gtatgaaccg
    21781 tatctgatcc aaaaaggatt tttaaagcgt acacctagag ggcgaattgc tactgcttat
    21841 gcctatgaac attttggtta cgactatcat atatgatttt tgcttttaga ttggaggaaa
    21901 atgaatgagt gatacaacac gtacgaaaca attgatcaaa aacgtattga ttgatttgtg
    21961 ccaaacgcaa tcttttcgaa aaatcagcgt ccaaaaaatc gcaaacgaag caaaaatcaa
    22021 tcgacaaaca ttttattatc attttacaga taaatacgat ttgcttcgtt gggtctattg
    22081 tgaggatgca ttgcattatt tagatccaca atgtctttca cttgataatt gggaagagca
    22141 agcattgaaa atgctgaaag caattgctga gaagaaagaa ttttatgcaa atactgtgct
    22201 ttctgaccca gaaattttac aaaaagagtt ttctcaactg atccaaaaat catttatcaa
    22261 gatttttgaa caaatggatg aagaaagaca actttctgca agagacaaac tattttatgc
    22321 tcgttttttt tcttacggtt gcagtggtgt attagtcaat tggatcacga atgaatatga
    22381 agaaacaccg atggaaatcg ctatgcagct tttccgttta gccaaggata cggaattata
    22441 ttcttatcgt ctttatgcaa aagaagagtt ggaagaacag taatagaaaa attttttgtt
    22501 ggaaaagcaa taatcgctaa aaggagatgg ggaaatgaca aaaattttat ttgtttgttt
    22561 gggaaatatt tgccgttcac cgatggcaga aggcttgctg agaaaacgaa tagcattaga
    22621 aggaagggaa gcagaatttt ttgtggattc tgctgctaca agcacgtatg aagttggaaa
    22681 gacaccgcac cctggaacga aaaaaatatt aaaccaagaa aaagtagata tgacgaatat
    22741 ggttgctcgg caaatcacac cccacgattt tgaaactttt gattggatca ttggcatgga
    22801 tcaagaaaat gtagaagaac tgaaacgaag agcaccacgc tcagcgcagg ggaaaatcca
    22861 tttgttttta agtagcattc ctggaaaaga aaaagaaaat gttcccgacc catactatac
    22921 caataacttt gatgagacat atcacttgat cacagaaggc ctgaatcatt ggtggaatat
    22981 ttggattgat gaaaataaaa taacaaacaa tttgtaaagt taacgctttc tttttaaggc
    23041 gatgttagag catttccttg tttttttgta aaatgttagg taaaataaaa ttaattaagt
    23101 aacactttct tagctaaagg aggagtgcat tcgatgaacg aagagacagt atcgccaatg
    23161 aagaagctta cgaagttagt tactcatact tcgtatcaac tttttgattc ttttgtaaat
    23221 gacactgaag aacatgctaa gaatgtacga ttgcaaggaa agtttattct tgagctgcaa
    23281 aaagctgcta ctgaacatag tctagttatc ttacaagtaa gagcaaatcg tttgagtagc
    23341 aagtatgaaa cgatctcagg ctgggtggcg acaaaaacgg tcgaaaaaga ccgagtgatg
    23401 atccgcctac aaaacgaaga gcagcagatc cggatggttc ctgtagaaca aattgaaaaa
    23461 ataacaatcc tttcaccagc aggggaacag gaacggttat caaaataatt tataccagat
    23521 agaaaagcac gtatgagctt gtaccatcaa tcattactaa tcaaaaataa ccgaatgctt
    23581 taagctgata tcagtaaaac ggaacgtttt aggatgagta tcatagcatg acagcattcg
    23641 gttgattagt tgaatcgatt ggatggtaca agtttttttg ataaaaaaag gtcctgctag
    23701 ccggggagct agcaggataa ggagttaaaa atgaaaaagt gttgttaggg ttgtttgttg
    23761 gtatgcttat ataatagtca ttagttgtga atattttgtg acgaaagttt tcgaaagttg
    23821 ttaagtt
//
LOCUS       DASHHB010000037        23810 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.038, whole genome shotgun sequence.
ACCESSION   DASHHB010000037 DASHHB010000000
VERSION     DASHHB010000037.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.038"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            137..994
                     /locus_tag="U2K71_001745"
     CDS             137..994
                     /locus_tag="U2K71_001745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HEM7315825.1"
                     /translation="MKKLKLFLPIIGLAILLSGCSKWIDRGESITAVGSSALQPLVET
                     VAETYQTENPGKFINVQGGGSGTGLSQVQSGAVDIGNSDLFAEEKSGIDASALVDHRV
                     AVVGITPIVNKDIGVKDISMENLKKLFTGEIKNWSEVGGKDQPVVILNRASGSGTRST
                     FEKWVLDGETAVQAQEQDSSGMVRQIVADTPGAISYVAFSYVSDDVDTLNVNGVEPTD
                     ENVTTNKWTIWAYEHMYTRDHPKKLTKEFLDYILSDEVQDNIVGELGYIPVSHMKVER
                     DWQGNIVKE"
     gene            1109..2029
                     /gene="pstC"
                     /locus_tag="U2K71_001746"
     CDS             1109..2029
                     /gene="pstC"
                     /locus_tag="U2K71_001746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HEM7315826.1"
                     /translation="MEDVKKVLLTKSKRSKMEQRGKFISFLCIALIVLVVLSIFYFVA
                     SKGLATFFTDKINVFDFLFGTDWNPSQIGANGKPMVGALPMITGSFIVTFLSAIVATP
                     FAIGAAVFMTEISPKNGTKILQPVIELLVGIPSVVYGFIGLSVIVPFVRSIFGGTGFG
                     ILAGTFVLFVMILPTVTTMTVDALKAVPRHYREASLALGATRWQTIYKVVLRAAVPGI
                     LTAVVFGMARAFGEALAIQMVIGNAALMPTGLTTPASTLTSILTMGIGNTIMGTVENN
                     VLWSLALILLLMSLFFNIVIRMIGKKGELK"
     gene            2029..2913
                     /gene="pstA"
                     /locus_tag="U2K71_001747"
     CDS             2029..2913
                     /gene="pstA"
                     /locus_tag="U2K71_001747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010764435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HEM7315827.1"
                     /translation="MNAKRSDKIATGVLYIVSGIIVLILAALLLYILVRGIPHISWSF
                     LTEPSKAYQEGGGIGIQLFNSIYLLLITMIISFPISLGAGIYLSEYAGKNWLTDVIRT
                     SIEILSSLPSVVVGLFGFLVFVIQFQYGFSIISGALALTVFNLPLLTRTVEESLQAVH
                     YTQREAGLALGLSRWETVIKVVVPEATPGILTGVILSSGRIFGEAAALIYTAGQSAPA
                     LDFTNWNPLSVSSPISIFRQAETLAVHIWKINTEGTMPDGVSVSAGASAVLIIAVLLF
                     NFGARAIGKRLYRKMTSA"
     gene            2928..3734
                     /gene="pstB"
                     /locus_tag="U2K71_001748"
     CDS             2928..3734
                     /gene="pstB"
                     /locus_tag="U2K71_001748"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002408906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEM7315828.1"
                     /translation="MKKYNWNETNVITMPKEKEVALYTDDLHVWYGDNEAIKGVDLEF
                     EKNKITALIGPSGCGKSTYLRSLNRMNDGIANTKITGKIMYQGVDINAPQVDVYEMRK
                     RIGMVFQRPNPFSKSIYDNITFALKQHGEKNKKKLDEIVETSLKQAALWDQVKDSLDK
                     SALALSGGQQQRLCIARAIAMKPDILLLDEPASALDPISTGTVEETLVNLKEDYTIII
                     VTHNMQQAARISDYTAFFYLGKVMEYDKTNKIFTRPKIQATEDYVSGHFG"
     gene            3750..4508
                     /locus_tag="U2K71_001749"
     CDS             3750..4508
                     /locus_tag="U2K71_001749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317877.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HEM7315829.1"
                     /translation="MTKEIISSKDLHLYYGKKEALKGINLSFNQGEITAMIGPSGCGK
                     STYLRSLNRMNDLIPNVTITGSVLYKDKDIYGPKTDVVELRKEIGMVFQQPNPFPFSI
                     YENVIYGLKLKGEKDKKVLDQVVEESLKAASVWDDVKDKLHKSALSLSGGQQQRVCIA
                     RVLAVNPEIILLDEPTSALDPVSTGKIESMLLELRERYTMIIVTHNMSQASRISDKTA
                     FFLDGHLIEYNDTKKIFMNPEKQETEDYISGRFG"
     gene            4521..5198
                     /gene="phoU"
                     /locus_tag="U2K71_001750"
     CDS             4521..5198
                     /gene="phoU"
                     /locus_tag="U2K71_001750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010723432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HEM7315830.1"
                     /translation="MLRTQFEEELLNLHNQFYEMGMMVSSAVHKSVRAYIKHDKILAQ
                     EVIENDININNMETRLEKKSFEMIALQQPVTTDLRMIITVMKASSDLERMADHAVSVA
                     KSTIRVKGQTRIPEIEKEISDMSDYVKKMVDNVLVAYVKTDEKDARTIANMDQRVNDY
                     FNRIYNATIKNMQENPETVISGTDYLNVASYLERIGDYVTNICEWIVYLATGKITELN
                     TNHNEIF"
     gene            5436..6467
                     /locus_tag="U2K71_001751"
     CDS             5436..6467
                     /locus_tag="U2K71_001751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEM7315831.1"
                     /translation="MQKKISIITIFFICVLTSWVIQHQAALYEVPVIEIEKVRIKEDH
                     QLITGRLMNTDQKGESIYLSVPYQKNQIETANIKARQMYLVQMHGDEWQLVAKKNDGW
                     LFFFTSISIGAMLLIGGKQGLFALGSLIINIVLLLLLLLIFTKTEGISLLNISILFVV
                     IGMIISILALDGWNRSSIIKISAAVTSVFLAFFICLLTMNHWKDQGLRYEELNYLTRP
                     YRSVFLSSVLIGTAGGTLDTVITVIATLEELTKRQPEITAKQLWLSGRQVGRDVIGSM
                     TNILLFVYISGSVPSLLLYLGNQWSFKETIEQHLSLEFLRVIAGSLGIVLSIPISVLF
                     FLTVRRLKR"
     gene            6464..7231
                     /locus_tag="U2K71_001752"
     CDS             6464..7231
                     /locus_tag="U2K71_001752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YibE/F family protein"
                     /protein_id="HEM7315832.1"
                     /translation="MNVLIILVGILAISVLLIGGTQGMYILLGLFINLGIFFLLLFGY
                     HQKWPILALSVIGFLLIAVVILFFINGYNLKMRAAFVSILIFLFCFLLLLPITDFLAI
                     QGFTSVELEELSGLDKTLAIDFRLLTRSLLLISLSGAVLDASVAISSGTFEVYQANPQ
                     LSFYQLRHSSFAIAKKILASTVMTLLFAFMGSSLALILWFIDLDIPFQQVINEKSFVL
                     EYTMAILTTLSALLVLPLTCVTTAYLFTKKKEQLKME"
     gene            7331..8899
                     /gene="liaX"
                     /locus_tag="U2K71_001753"
     CDS             7331..8899
                     /gene="liaX"
                     /locus_tag="U2K71_001753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="daptomycin-sensing surface protein LiaX"
                     /protein_id="HEM7315833.1"
                     /translation="MKERERILDLVKKGILSTEEGLDLLESMATEKDEKQIKKEADKV
                     TASHKEKDQASQLIDKLENGEEEISEPVDPKERERQDQENLEKILDELATEANKTSAR
                     LDEVNAQFADNKAARNEKQEALMQLNTKEELGELTEEELAQRQTLEAEIKELEASGDT
                     LLEEQLKLEAELKDIRKNQWSEKKETFTDKFELPDDWKDQATDTLNQVGEKMSEAGSQ
                     LGKFLKKTFQTVSETVNDNMEWKDVSLRVPGIATTKFEHEFYYEAPAASILDIKAANG
                     NVTLKTWDSNDVKVEAKIKLYGKMGAEPFEAFSERSQIEVNEDHISFQIPNKRVRADL
                     VFYLPKRVYDHAAIKLLNGNIMIETLEAKDIYTKSTNGNIIVDQLTATMLEVEGVNGN
                     IDIRNGNILDSIIETVNGTVTFGATAENLSVSLVNGDVRLTIKEDNLKKVEASSVNGN
                     VKVALPDGIGLEGHAKTSLGSINSRLSNYEVVREKKERTNQMLQFRRVSDGEITQVQL
                     STTTGSIYLKDTDK"
     gene            9196..9516
                     /locus_tag="U2K71_001754"
     CDS             9196..9516
                     /locus_tag="U2K71_001754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HEM7315834.1"
                     /translation="MMKKKLTKSNKNVVLTGTLAGIGEYIGIDPTVIRVLYVFLSLVA
                     FGSPIILYILLALIIPSARSARTNYGHNNAYYEKNNYREQTREQTKKRKEAEKIDDDE
                     WSDF"
     gene            9516..9866
                     /locus_tag="U2K71_001755"
     CDS             9516..9866
                     /locus_tag="U2K71_001755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HEM7315835.1"
                     /translation="MSYFQRILVNTLTFISLAVLFPAMVHVSSIWTAVLASFVLSVLN
                     MFVKPILTILSLPFTLVTLGLFTFVINAAILKMTSFFVGAEHFSFSSFGAALLVAILM
                     SLVNVIVSEHNSRK"
     gene            10033..10971
                     /locus_tag="U2K71_001756"
     CDS             10033..10971
                     /locus_tag="U2K71_001756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HPr kinase/phosphorylase"
                     /protein_id="HEM7315836.1"
                     /translation="MSEVVKVSQLVKKLSLEIVTGDEKSLDRVIVTGDISRPGLELTG
                     YFNYYSHNRLQLFGSKEISFAERMIPEERLIIMRRLCSEDMPAFIISRGLPAPEEMIQ
                     AANENGIAVLRSPISTSRLLGELSSYLDGRLAPRTSVHGVLVDVYGLGVLIQGDSGIG
                     KSETALELIKRGHRLIADDRVDVYQQDELTVIGEPPKILEHLIEIRGIGIIDVMNLFG
                     ASAVRGSMQVQLAVYLEAWAKDKKYDRLGSEDTTVEIAEVGIPQIKIPVKTGRNVAII
                     IEVAAMNFRARTMGFDATKTFEERLSRLIEENSGND"
     gene            10986..11816
                     /locus_tag="U2K71_001757"
     CDS             10986..11816
                     /locus_tag="U2K71_001757"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HEM7315837.1"
                     /translation="MLAQINRVAFELVGIPIYWYALIIVSGIIIAMWLSSREAVRVGL
                     KADDVTDFMLWGLPISIIGARLYYVLFDLPQYIADPIQIFNIRSGGLAIYGGLIAGAI
                     TLYFFTKYHFISLWTFLDIAAPSVLLAQAIGRWGNFMNHEAYGPDTTRSFLESLHLPQ
                     FIIDNMYIEGIYRQPTFLYESLWSLIGFILLLFLRKKPHLLKKGEVLLCYLMWYSFGR
                     FFIEGLRTDSLYFLGVIRISQLLSAVLFVGTLILFIWRRKKGNLPDYDRSLGKNQQII
                     "
     gene            11860..12885
                     /locus_tag="U2K71_001758"
     CDS             11860..12885
                     /locus_tag="U2K71_001758"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HEM7315838.1"
                     /translation="MNYRVAVLGPGSWGTALAQVLAENGHDVRIWGHRASQVEEINTQ
                     HTNQRYLPDHYLPDSIQAFTNMEDAVKDADAVLFVIPTKAIRSVAKELVPKLNTKPVI
                     IHASKGLEQGTHKRISDILLEEIPAEKRKAVVVLSGPSHAEEVAKHDITTITAASTDA
                     KAAKYVQELFMNNYFRIYTNPDVIGVEMGAALKNIIAIGAGAIHGLGFGDNAKAAIMT
                     RGLAEISRLGVAMGANPLTFIGLSGVGDLVVTCTSVHSRNWRAGNLLGQGQPLEEVLA
                     NMGMVVEGVNTTQAAMELAQIMEIEMPITQTIYEVLYEQKEIKTAAKEIMLRDGKKEN
                     EFSLTIF"
     gene            12900..13838
                     /gene="galU"
                     /locus_tag="U2K71_001759"
     CDS             12900..13838
                     /gene="galU"
                     /locus_tag="U2K71_001759"
                     /EC_number="2.7.7.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UTP--glucose-1-phosphate uridylyltransferase
                     GalU"
                     /protein_id="HEM7315839.1"
                     /translation="MKVKKAVIPAAGLGTRFLPATKAMAKEMLPIVDKPTIQFIVEEA
                     LKSGIEDILIVTGKAKRPIEDHFDSNIELETNLSEKGKTELLKLVEETTDVNLHFIRQ
                     SHPKGLGHAVLQARAFIGNEPFVVMLGDDIMEDEVPLTQQLIDDYERTHASTIAVMQV
                     PHDETSKYGIINPGEVLEDGLYNVKNFVEKPDPSKAPSDLAIIGRYLLTPEIFDVLSE
                     QQPGAGNEIQLTDAIDTLNKTQRVFARKFTGKRYDVGDKFGFMKTSIEYGLTHPEVGA
                     PLREYILSLGKKLEAEGSKIDHNPKGITEIKEEKKK"
     gene            13963..14403
                     /locus_tag="U2K71_001760"
     CDS             13963..14403
                     /locus_tag="U2K71_001760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF948 domain-containing protein"
                     /protein_id="HEM7315840.1"
                     /translation="MSGGEIAGLIAAIAFAILVVFIVRVLLQVSKTLEKVDQTVAEAN
                     TTIEIVTKDVDLLSRQVEGLLVKSNELLLDINGKVATIDPLFTAVADLSESVSELNHS
                     SKNIATKVGGIGKTTAKATVAGKVGGTAMKFFKNKKHTETTGGK"
     gene            14406..15008
                     /locus_tag="U2K71_001761"
     CDS             14406..15008
                     /locus_tag="U2K71_001761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEM7315841.1"
                     /translation="MAKKGGFLLGAVVGGTAAAIAALLLAPKSGKELRDELAAQADDL
                     MDMASDYTDMAKEKSTELTGIAKEKAQDLSQQAGDLAGSVKQKVSESADEAGSESDDI
                     LLNLKEQSADLSNRFKKTAEDMKKPADELGSIAKDTSEDIMIDVKDAAGKAGDTVNEG
                     MSEAKPVADDTKEMMKMAKEDAKEAKKEIKDTMTDSSTNH"
     gene            complement(15076..16182)
                     /locus_tag="U2K71_001762"
     CDS             complement(15076..16182)
                     /locus_tag="U2K71_001762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HEM7315842.1"
                     /translation="MNNVKINELRQWMAQHQVELAYISDPGHIAYFSGYESDPHERVL
                     ALFIPLEQDPFLFTPALEVEDAQNSSWAYDVRGYLDSENPWEIIGKELRARYGQPGNV
                     GIEKNALTVERFDAISDILSGTDSYTDLASIIQRLQLIKTLEEKDKLIEAGKWADVAF
                     EIGFKAVKEGVTEQAIIAEIEYQLKKQGVSQMSFDTLVLAGANAASPHGTPGNTKISP
                     NELVLFDLGVVWNGYCSDATRTIAYQKPTDFQEKIYNITLEAQLAAQEAVRPGVTAGE
                     LDQIARNVINSYGYGEYFNHRLGHGIGTTVHEFPSLVEGNDLVIEEGMCFSLEPGIYI
                     PEKVGVRIEDCVYVTSDGCVPFTTTPKELLVLEK"
     gene            16392..17393
                     /gene="ccpA"
                     /locus_tag="U2K71_001763"
     CDS             16392..17393
                     /gene="ccpA"
                     /locus_tag="U2K71_001763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="catabolite control protein A"
                     /protein_id="HEM7315843.1"
                     /translation="MEKQTITIYDVAREANVSMATVSRVVNGNPNVKPATRKKVLEVI
                     DRLDYRPNAVARGLASKKTTTVGVIIPDVSNMFFASLARGIDDVATMYKYNIILANSD
                     GNDQKEVNVLNNLLAKQVDGVIFMGHHITDEIRGEFSRSKTPVVLAGSIDPDEQVGSV
                     NIDYVSATKDAVNKLAKSGNKKIAFVSGALIDPINGQNRLKGYKEALSENNLPYSEGL
                     IFEAQYNFKDGLSLVDRIHNSGATAVYVSDDELAIGILDGLLDRGVKVPEEFEIITSN
                     NSLLTEVARPRLTSITQPLYDLGAVSMRLLTKMMNKEEVEEKTIILPYGIEEKGSTK"
     gene            complement(17729..20074)
                     /locus_tag="U2K71_001764"
     CDS             complement(17729..20074)
                     /locus_tag="U2K71_001764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HEM7315844.1"
                     /translation="MPRKNTRKKQNRRKKEKWFVPKIIFRVFQSLTVFITVLLLMFAA
                     LGIGIGAGYFAYLVEDTQLPTKKALQTELGNITETSKIVYADNTEISKIQTDLMRTTI
                     SSDKISPLLKTAIISTEDEYFDKHQGYVPKAVLRALVSEATGIGSSGGSTLTQQLVKQ
                     QILTDETTFKRKANEIILSAQVEKYFSKDEIIATYLNVSPFGRNNKGQNIAGVQEAAR
                     GIFGVDAKDVTLPQAAYIAGLPQSPITYSPYTNTGALKEDLSAGLARKDFVLFSMYRE
                     GQITKEQYEEAKAYDLTKDFLPQQIAEQTDREFLYYTVMNEATRIIAQQLAEKDNADM
                     SDSSVSDAYYQKAQQTIQNKGYTIHSTIDKDVYAALQNGVENYGYMLDDGAGSQVETG
                     NVLMDNRTGRIYGFVGGRNYSQNQNNHAFDTERQAGSSIKPVLVYGPAIDMGLIGSES
                     RVSDYATTWQEGENAGEKIVNATNEGSNTFQTVRESLEWSNNIPAYHLYQDVLNSGGS
                     KQYAYEHYLAKMNYPANDNWGVESAPLGTVDVTTLQQTNGFQALANGGVYEEGYIIDS
                     ITDNAGNVIYKHESNSVRIYSEATASIMNDMMRSVINAKITTPFKDAISSLNGNLGKA
                     DWVGKTGSTNEYRDSWLIVSTPSITISSWAGHDDNTGMDSKARIRSANYLANLINQAY
                     QAKPDIFGTDEKFELSSDVMKKKVSGFTGQTPGKVTVNKKSIQTPGKTVESLWAKNGP
                     KKSAFKFGVGGTDENYTDYWNTASAYARANQATKESDEKDD"
     gene            20353..21255
                     /locus_tag="U2K71_001765"
     CDS             20353..21255
                     /locus_tag="U2K71_001765"
                     /EC_number="3.1.21.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribonuclease IV"
                     /protein_id="HEM7315845.1"
                     /translation="MLLGSHVSMSGKKMLLGAAEEAASYGSSTFMIYTGAPQNTRRKA
                     IEEMNIDAGKAYMAENGLTNIVVHAPYIINLGNTIKPEMFPFAVQFLREEIVRAEALG
                     ATQITLHPGAHVGAGTEAGIARIIEGLNEVLTKEQTAQIALETMAGKGTEIGRTFEEL
                     AQIIDGVKLNEKLSVTFDTCHTNDAGYNIKEDFSGVMEEFDKIIGLDRLKVLHINDSK
                     NPQGSHKDRHANIGFGTIGFDTLNKIVHDPMFDNISKILETPYVGKDKKKAYAPYGKE
                     IAMFKAQIFDPEVFPELLAEEVIL"
     gene            21468..23243
                     /locus_tag="U2K71_001766"
     CDS             21468..23243
                     /locus_tag="U2K71_001766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosyltransferase"
                     /protein_id="HEM7315846.1"
                     /translation="MLNTLKPLINQLKKKHTYFILGIALLFTIIRLYLYSKAIYGTDM
                     SATYDDQLLIRYAQNILDGKWLGEYTTTTLSKGISYSLFLVLAHKLHLSYAVLFGMFS
                     IFASILVALAFKPIVKNKYLLLSFYFYFLFSPISFTHEYSTRTYRNALVIPAVEIIIA
                     CLLAIYYRKYSSSKKMLPWFLGLAVSFPFFWFIREDSIWLLPFTVVALGISFVQILLG
                     QDFSFKEAKKLKERPFLLSKQEWLKLSLFLLPLVGFSIVYQWIEYKNETTYSIAAIND
                     RSSGEFGRLMKQLIRIDDETNLNKENSEIWVSHAALNKALDASPTFHAVSTRIDELYT
                     THAWTQGGKVKELPGDIIFWALRDVVSESGYYQNNAKETNDFWKKVNQELEEAYQKGT
                     IKKKKEFYLMGSGDGKTMNDLPIVFAFLKSGYAYAWRYKDFHQGGNYSFGTQEQVEQA
                     QELLNLPLMDNWIDSSDPRTPDFQLTKTAKVANVMIKIYQVLAIPVLIASIAGILLMI
                     LGTAFAKADRAYYSSSLIVVTGLILTQLVFLFGVSWFCSYAPEKKDYFLSVYTGAGVP
                     LTQFIVVTSMLVFSKLNLKKALSSK"
     gene            23500..>23810
                     /locus_tag="U2K71_001767"
     CDS             23500..>23810
                     /locus_tag="U2K71_001767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM7315847.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEENTLCSWKTDKSD
                     A"
BASE COUNT         7596 a         4230 c         5016 g         6968 t
ORIGIN      
        1 agcatagaaa cttttaatga gatagttttt tttacaatta tgagaattta ttttgttatt
       61 taatgatttt gattcgtttt catcaagtaa tgaaaatgat aaaataaaca tactttagga
      121 ttaaaggaga tttctaatga aaaaacttaa attattctta ccaataatag gattagccat
      181 tcttttatca ggctgttcaa agtggattga tcgaggagaa tccattacgg ctgttggctc
      241 ttctgcttta cagcctttag tagaaacagt tgctgaaact taccagacag aaaatcctgg
      301 aaaattcatc aatgtacaag ggggcggaag cggaactggt ttgtctcaag tacagtcagg
      361 agcagtcgac atcggaaact ctgatttgtt tgcggaagaa aaatcgggga tcgatgcctc
      421 agcattagtc gatcatcgag tagcagttgt tgggatcact cctattgtga acaaggatat
      481 tggcgtaaaa gacatcagta tggaaaacct gaaaaaacta tttacaggcg agatcaaaaa
      541 ctggagtgaa gtcggtggaa aagatcagcc agtagttata ttgaaccgcg cttcaggaag
      601 cggaacccgt agcacgtttg aaaaatgggt attggacgga gaaacagctg ttcaggcaca
      661 agaacaagat tcaagcggaa tggtgcgcca gatcgttgcc gacacacccg gtgcaatcag
      721 ctatgttgct ttttcttatg tttccgatga tgtagatacg ttgaatgtta acggtgtaga
      781 accgacagat gaaaatgtga caacgaacaa atggacgatt tgggcatatg agcatatgta
      841 cacaagagat catccaaaaa aactgacaaa agaattttta gactatatct tatctgatga
      901 agtacaagat aacatcgttg gagaactagg ttatatccct gtttctcata tgaaagtcga
      961 acgtgattgg caaggaaata tcgttaaaga gtaatcttta cacaaaattt acaaacttag
     1021 ctaaactttt acatgttatt aaaaccaaat tgatgttcaa tttgtacact gagtgagtat
     1081 gttaaacctg attaaggggg agtcatctgt ggaagatgtg aaaaaagtat tattaacaaa
     1141 atcaaaacgt tcaaaaatgg aacagcgtgg aaaattcatc agttttttat gtatcgcgct
     1201 gatcgtgttg gttgtattat ctatttttta ttttgtagct agtaaaggat tggccacctt
     1261 ttttactgat aagatcaatg tatttgattt tctgtttggt acagattgga atccaagcca
     1321 aatcggagca aatgggaaac caatggtagg ggcattgcct atgatcacag gttcgtttat
     1381 tgtgaccttt ttatctgcta tcgttgccac gccatttgcc attggtgcag cagtattcat
     1441 gacggagatc tctcctaaaa acggaacgaa gatcttacag ccggtcatcg agctgcttgt
     1501 aggtattcct tctgtcgttt atggttttat cggtttatca gttatcgtgc cttttgtcag
     1561 aagtattttt ggcgggacag ggtttggtat cttagctggt acatttgtgt tgttcgttat
     1621 gatcttgccg actgtcacaa cgatgactgt tgatgcatta aaggcagtgc cacggcacta
     1681 tcgggaagct tcattagcgc ttggtgccac aagatggcaa acaatctaca aagtcgtgct
     1741 acgagcagcc gtgcccggga ttttaacagc agtcgttttt ggtatggctc gtgcgtttgg
     1801 tgaagcctta gctatccaaa tggtcatcgg taatgcagca ttgatgccaa caggacttac
     1861 tactcctgct tctacattga cgtccatctt aacaatggga atcggaaata caatcatggg
     1921 aacagttgaa aataatgtgt tatggtcatt agcgctgatc ttgctattga tgtcattatt
     1981 ctttaatatc gtgatccgta tgattgggaa gaaaggagag ctgaaataat gaacgcaaaa
     2041 cgctcagata aaattgctac aggtgtttta tatatagttt cggggatcat cgtattgatt
     2101 ttagcagctt tactacttta tatcttagtt cggggaatac cgcatatttc atggtccttc
     2161 ttgaccgaac cttccaaagc ctaccaagaa ggcggtggaa tcgggatcca gctatttaac
     2221 tcgatttatt tgcttttgat cacgatgatc atcagttttc ctatttcttt aggtgcgggt
     2281 atctatcttt cagaatatgc tggaaaaaac tggctaacag atgtgattcg cacgtcgatt
     2341 gaaatcttga gctcgttgcc ttctgtcgtt gtcggtttat tcggtttctt ggttttcgtt
     2401 attcaattcc agtatggttt ctcgattatt tctggtgcat tggctttgac tgtattcaac
     2461 ttgcctcttt tgactagaac agttgaggag tctttacaag cggtccatta cacacaacga
     2521 gaagcaggat tggccttagg tttgtcacgc tgggaaacag tgataaaagt ggtggttcca
     2581 gaagcgacac cagggatttt gactggtgtg atcctaagtt ctggaagaat ttttggtgaa
     2641 gcagctgcat tgatctatac ggctggacaa agtgcacctg cgttagattt tacgaactgg
     2701 aatccattaa gtgtttcaag cccgatcagc attttccgtc aagctgaaac attagctgtc
     2761 catatttgga agatcaatac agaaggcacg atgcctgatg gtgtttctgt ttcggctggc
     2821 gcttctgccg tgttgatcat tgctgtttta ctgttcaact tcggcgctcg tgcgattggc
     2881 aaacgattat accgaaaaat gacttcagcc taaggagaag aagcaaaatg aaaaaatata
     2941 actggaatga aacaaatgtc atcaccatgc caaaagagaa agaagtagca ctatatacgg
     3001 atgatctaca tgtctggtat ggcgacaatg aagcaatcaa aggagtggat ctggaatttg
     3061 aaaaaaataa gatcactgca ctgattggtc cttccggctg cggaaaatct acgtatcttc
     3121 gttcgttgaa tcgaatgaat gatgggatcg ctaatacgaa aatcactggg aaaatcatgt
     3181 atcaaggcgt agatattaat gcgcctcaag tggatgttta tgagatgcgc aaacggattg
     3241 gcatggtttt tcaacgtcct aatccgttca gcaagtcgat ttatgacaat attacatttg
     3301 ctttgaaaca gcacggcgaa aaaaacaaaa agaaattaga tgaaatcgtt gaaacaagct
     3361 tgaaacaagc tgccttgtgg gaccaagtaa aagacagctt ggacaaaagt gccttggctt
     3421 tatctggcgg gcaacagcaa cgtttatgta ttgctcgtgc gatcgcgatg aaacctgata
     3481 tcttgctgct agacgagccg gcaagtgctt tggaccctat ttcgacagga acggttgaag
     3541 aaacacttgt aaacttgaaa gaagactata cgatcatcat cgtgacgcat aacatgcaac
     3601 aagcagctcg tatcagcgac tatactgcct ttttctatct aggaaaagta atggaatatg
     3661 acaaaactaa caagatattt accagaccaa aaattcaggc aaccgaagat tatgtgtccg
     3721 gtcattttgg ttaagacgga ggcctcaaca tgacaaaaga aattatttca tctaaagatc
     3781 ttcatctgta ttatggaaag aaagaagcac taaaagggat caatctaagt ttcaatcaag
     3841 gggaaatcac tgcgatgatc ggtccttctg gttgcgggaa atccacttat ctgcgttctt
     3901 tgaaccggat gaatgacctg attccgaatg taacgatcac cggaagtgtt ttatataaag
     3961 acaaagacat ctatggtcca aaaacagatg ttgttgaact aagaaaagaa atcggcatgg
     4021 tattccagca gccaaaccca tttccttttt ctatctatga aaatgtcatc tatggattga
     4081 aattaaaagg ggaaaaagat aaaaaagtcc ttgatcaagt agttgaagaa agcctgaagg
     4141 cagcgtctgt ttgggatgat gtgaaagaca aattgcataa aagtgcttta tctttatccg
     4201 gtgggcagca gcagcgtgta tgtatcgcac gtgttttggc agttaatccg gaaatcatct
     4261 tattggatga accaacaagt gcgcttgatc ctgtttctac tgggaaaatc gaaagtatgc
     4321 tgttagaatt aagagagcgg tatacgatga tcatcgtgac ccataatatg tctcaagctt
     4381 cacgtatttc agataagacg gcattctttt tagatggtca cttgatcgaa tacaacgata
     4441 cgaagaagat attcatgaat ccagaaaaac aagagacaga agattatatt tcaggtcgat
     4501 ttggataagg aggaaacgga atgctacgta cacaatttga agaagaattg ctgaatctcc
     4561 ataatcagtt ctacgaaatg gggatgatgg tgagcagcgc cgtgcacaag tcagttcgtg
     4621 catacatcaa acacgataaa atcttagccc aagaagttat cgaaaacgat ataaatatca
     4681 ataacatgga aacacgcttg gagaagaaaa gttttgaaat gatcgcattg caacagccag
     4741 ttacaactga tttgcgtatg atcattacgg tcatgaaagc tagctcggat ttagaacgta
     4801 tggccgatca tgcggtgtct gttgctaaat ccacgattcg cgtaaaaggg cagacgagaa
     4861 taccggaaat cgaaaaagag atctccgata tgtccgatta tgtgaaaaaa atggtggaca
     4921 acgtcctagt tgcttatgtc aaaacagacg aaaaagacgc acgtacgatc gccaatatgg
     4981 atcaaagagt caatgactat ttcaatcgaa tctataatgc aacaatcaaa aatatgcagg
     5041 aaaatcctga gacagtcatc agtggaacag actacttgaa tgttgccagt tatctagaac
     5101 ggatcggaga ctatgtgaca aacatctgtg aatggatcgt ctacttggct acagggaaaa
     5161 tcactgagtt aaatacgaac cacaatgaga ttttctaaac tgaagctgtg attatgccgt
     5221 ttgtttagga aaagaggttg tgaaatcagc ggttgtgaaa aagctgttct gcatcaagta
     5281 ataagaacag tcaaacaaaa aatagctcct catatttttc gtttggctgg acttattacc
     5341 gcagatgcaa gcttttgaac accgtttatt cgggaaaggg tttgtgacaa aacatgtcac
     5401 aaaccctttt catcatttca tagaaaggat aaagaatgca aaaaaaaatt agcatcatca
     5461 ctatattttt tatttgtgtg cttacttcat gggttattca gcatcaagct gctttatatg
     5521 aagtacctgt catcgagatc gaaaaagtac gaataaaaga ggatcatcaa ttgattacag
     5581 gaagactgat gaacacggat caaaaaggag aatcgattta tttatcggtt ccttatcaaa
     5641 aaaatcaaat agaaacagca aatataaaag cgagacaaat gtatctagtc caaatgcatg
     5701 gagatgaatg gcagctagta gcaaaaaaaa atgatggctg gcttttcttc ttcacaagta
     5761 tctccattgg cgccatgttg cttattggcg gaaaacaggg tctatttgct ttaggaagtc
     5821 tcatcatcaa tatcgtttta cttttacttt tgttgctcat ctttactaaa acagaaggga
     5881 tttctctatt aaatatatct atcctgttcg tcgttatagg gatgattatt agcatattgg
     5941 ctttagatgg atggaataga agcagtataa tcaaaatttc agctgcagtt acgagtgttt
     6001 ttttagcatt ttttatttgt cttttgacaa tgaatcattg gaaagaccaa ggactgcgct
     6061 atgaggaact gaattatctg acacgaccgt atcgttctgt ttttctttct agcgtactga
     6121 ttggaacagc tggaggaaca ctcgataccg ttatcacagt cattgcgaca ttagaagaat
     6181 tgactaagcg acaaccagaa ataacagcga aacagttatg gctttcagga aggcaagtgg
     6241 gtcgagatgt gattggctcg atgaccaata tccttttgtt cgtctatatc agtgggtcag
     6301 ttccatcgct tcttttatat ttaggaaatc aatggtcgtt caaagaaacg atagaacagc
     6361 atttatcatt ggaattttta cgcgtcattg ctggcagctt aggaatagta ttatcgatcc
     6421 ctatctcggt cctctttttt ctgactgtaa ggaggctgaa acgatgaatg tacttattat
     6481 acttgttggg attttagcaa tcagcgtgct acttattggc ggtacgcaag gtatgtatat
     6541 tcttttaggc ttattcatca atttagggat ctttttcctg ttgttattcg gttatcatca
     6601 gaagtggccg atactggcat tgtcggtcat aggattttta ttgattgctg tggttatctt
     6661 attttttatc aacggttata atctaaaaat gcgtgcagct tttgtgagta tactgatctt
     6721 tttattttgt ttcctactat tgcttcctat cactgatttt ttagcgatcc aaggttttac
     6781 atcggtagaa ctagaagaat tatctggctt agataagact ttagcgattg attttagatt
     6841 acttacgaga tcgttgcttc tgattagtct tagtggtgct gttttagatg ctagtgtagc
     6901 aatttctagt gggacattcg aagtatatca ggcaaatccg caattatctt tttaccaatt
     6961 gcgccattct agtttcgcta ttgctaaaaa aatattggcg tcaacagtaa tgacacttct
     7021 ttttgcattc atgggaagtt ctttagcgtt gatcctctgg tttattgatt tagacattcc
     7081 ttttcagcaa gtgatcaatg aaaaatcatt tgttctagaa tatacgatgg ctattcttac
     7141 cacattgtct gcactacttg ttcttccttt aacctgcgtg actacagcat atctctttac
     7201 aaaaaagaaa gaacaactca agatggagta aaagttacaa cttaagtcct atgacatttt
     7261 tactgaatca gtgcataata tggatgtaag gaaaagcttg caaacaattt tgcttaagga
     7321 ggcaatttcc atgaaagaaa gagaacgtat tttagattta gtgaaaaaag gaatcctttc
     7381 gactgaagaa ggcttagatt tattagaaag catggcaact gaaaaagacg aaaagcaaat
     7441 aaaaaaagaa gcagacaagg taactgctag tcataaagaa aaagatcagg caagtcaatt
     7501 gatcgataaa cttgaaaacg gcgaagaaga aatttctgag ccagttgatc caaaggaaag
     7561 agaaaggcaa gatcaagaaa acttagaaaa gatcttagat gaactagcaa ctgaagcgaa
     7621 taaaacttcg gcgcgtctag atgaagtaaa tgctcaattc gctgacaata aggcagcacg
     7681 caatgagaaa caggaagcat tgatgcagct gaatacaaaa gaagaattag gcgagttgac
     7741 agaagaagaa ttagcacagc gtcagacgtt agaagcagaa atcaaagaat tagaagctag
     7801 tggcgataca ctgttagaag aacaattgaa attagaagca gaattaaaag atatccgtaa
     7861 gaatcaatgg tctgagaaaa aagaaacgtt tactgataag tttgaattgc cagatgattg
     7921 gaaagatcaa gcaacagata cactgaatca agtaggcgaa aaaatgtcag aagctggatc
     7981 acagttaggc aaattcttga agaaaacatt ccaaactgtt tcagaaacag tgaatgacaa
     8041 catggaatgg aaagatgtca gcttacgtgt ccctgggatt gcgacaacga aatttgagca
     8101 tgaattttac tatgaagctc ctgcagcaag tatccttgat atcaaggcag caaacggaaa
     8161 cgtgacgtta aaaacgtggg acagcaacga tgtgaaagtt gaagctaaga tcaaacttta
     8221 cggaaaaatg ggtgctgaac catttgaagc attctctgaa cggagccaga ttgaggttaa
     8281 tgaagatcat atctccttcc aaattccaaa taaacgtgtg cgtgcagacc ttgttttcta
     8341 cttacctaaa cgagtatacg accacgcagc tattaaactt ctaaatggca acatcatgat
     8401 tgaaacattg gaagctaaag atatttatac aaaatctacg aatggtaaca tcatcgtgga
     8461 ccaattaact gcaacgatgc tggaagtaga aggtgtaaac ggcaatatcg atattcgaaa
     8521 cgggaacatc ttagattcta ttatcgaaac agtaaatggt acagtgacat ttggtgcgac
     8581 agcagaaaac ctttctgtgt ctttggtaaa tggtgatgta cgtcttacga ttaaagaaga
     8641 taatttgaaa aaagtagaag caagttcagt gaacggaaat gtcaaagtcg ctttaccaga
     8701 tggcatcggt ttggaaggac atgctaaaac aagtcttgga agtatcaaca gccgtctgtc
     8761 caattacgaa gtagttcgtg agaaaaaaga acggacaaac caaatgttgc aattccgtcg
     8821 tgtttcagat ggtgaaatca cgcaagtgca actttcaacg acaacaggaa gtatctattt
     8881 aaaagataca gataaataaa gaggttgtga aagagatgtt cagccttgag caataagaag
     8941 cgattttgta aaatagcttc ccctatttta caaaattgtg ccttattgcc gataggctat
     9001 ctcttgaaca ccgtttacca agagattatg aacctgccaa ttatcgccga gtcacaagga
     9061 ataatttcag aaaaagctcg tcacgtttta tgaatatggt aatttgtgac cgagaatttt
     9121 gcagaaagac accgattaag aagaaggttg tcacaacgat tttcttcatt taaaatagta
     9181 gaacagaggt gagacatgat gaaaaaaaag ttgacaaaat ccaataaaaa tgttgttttg
     9241 acagggactt tagcaggaat aggagaatac atcgggattg atccgactgt tatccgtgtg
     9301 ctttatgtat tcctgagctt agttgctttt ggaagtccaa tcattttata cattttatta
     9361 gctttgatta tcccatctgc gcgttctgca cgaacaaact atggacataa caatgcatat
     9421 tatgaaaaaa acaattatcg tgaacaaact cgtgaacaaa caaaaaaacg caaagaagcg
     9481 gaaaaaatcg atgatgacga atggagtgac ttttaatgtc gtactttcaa cgtattttag
     9541 ttaatacgtt aacattcata tcattggctg ttctttttcc tgcgatggtc catgtgagca
     9601 gtatttggac ggccgttctt gccagcttcg tattgtctgt gttgaatatg tttgtcaaac
     9661 cgattttgac gatactgtcc ctgcccttta cattagtcac attgggattg tttacgtttg
     9721 ttatcaatgc agcgattctt aaaatgacct ccttttttgt gggagcagaa cattttagtt
     9781 tttcttcatt cggtgcagcg ttgttagtag ctatcttgat gtcgcttgtc aatgtaattg
     9841 tcagtgaaca taacagtaga aaataaaaga aagctgtgcc gaactatcgg cgcagttttt
     9901 ttatttagaa tcaggaatgc gttttctatt acattggtaa ttaaataaaa acgttttttt
     9961 taattggtag tacttttggc tagacaatga taaaatgata gagaacactc taatagaaat
    10021 gaggtagcat gaatgtcaga agttgtaaaa gttagccaat tagtgaaaaa attatctttg
    10081 gaaatcgtga caggcgacga gaaaagctta gaccgtgtca tcgtaactgg ggacatctca
    10141 cgcccaggac tagagttgac tggttatttt aattattatt cccataatcg tttacagcta
    10201 tttggcagta aagaaatcag ttttgcggaa cggatgatcc cagaagagcg tttgatcatc
    10261 atgagaagat tatgtagtga agatatgccc gcatttatta tttcaagagg attaccggct
    10321 ccagaagaaa tgatccaggc agcaaatgaa aatgggatag ctgtactgcg ttctcccatc
    10381 tcaacttctc gtttattagg agaattatcc agttatttag atggtcgcct tgcccctcga
    10441 acaagtgtcc acggtgtttt agtggatgtt tacggattag gtgtactgat tcaaggtgac
    10501 agtgggatag gaaaaagtga aacagcgttg gagttgatca aacgcggtca tcgattgatc
    10561 gcagacgatc gagtagatgt ttatcagcaa gatgaattaa cggtgatcgg agaaccgcct
    10621 aaaatcctgg aacatctaat tgaaatccga ggaatcggga tcattgatgt aatgaatctt
    10681 tttggtgcga gtgcggtacg tggctctatg caagtccagt tggctgttta tctggaagca
    10741 tgggcaaaag ataagaaata cgaccgtctg ggctctgaag acacgaccgt tgaaatcgcg
    10801 gaagtgggca ttccacaaat aaaaatccct gtcaaaacag gacgaaacgt tgcaatcatc
    10861 attgaagtag ccgcgatgaa tttccgtgca cgtacaatgg gctttgacgc aacgaaaaca
    10921 tttgaagaaa gattaagtcg cttgattgaa gaaaactcag gcaacgacta gcaaggagtg
    10981 acaatatgtt agcacaaatc aatcgagttg cttttgagct tgtagggatt cctatctact
    11041 ggtatgcatt gatcatcgta tcaggtatca ttatcgccat gtggcttagc agcagagagg
    11101 ctgttcgggt aggattaaaa gcagatgatg tgacagattt tatgttgtgg ggattgccta
    11161 tctccattat tggtgctcgc ttatattatg ttttatttga tcttccccag tatatagctg
    11221 atccaatcca aatattcaat atccgttcag gaggcttagc tatttatggc ggactgattg
    11281 caggcgcgat cacgctgtat ttttttacta aatatcattt tatttctttg tggacttttt
    11341 tagatatcgc tgcaccaagt gtattattag cacaggcaat cggtcgttgg ggaaatttca
    11401 tgaaccacga agcatatgga ccagacacga cgcgaagctt cttagaaagt ctgcatttac
    11461 cgcaattcat cattgataat atgtatatcg aagggatcta tcgtcaacca acatttcttt
    11521 atgaatcact ttggagtttg attggattta ttttgctttt gtttttacga aagaaaccac
    11581 accttttgaa aaaaggcgaa gtccttttat gttatctaat gtggtatagt ttcgggcgtt
    11641 tctttattga aggattacgt acagacagtc tttacttttt aggtgtgatc cgtatttctc
    11701 aattattatc tgctgtgtta tttgtcggta cattgattct atttatttgg cgcagaaaaa
    11761 aaggaaattt accggattat gaccgctcat taggaaaaaa tcaacaaatt atctgatttt
    11821 tagagcgtta aaaatgaaaa aaatttcagg agtggtaaaa tgaattatag agtagcggtt
    11881 ttaggaccag gctcttgggg tactgctttg gcacaagtcc tcgccgaaaa cggacatgac
    11941 gttcggattt ggggacatcg tgcttcacaa gtagaagaaa tcaacaccca acacacgaat
    12001 caacgttatt taccagatca ttatctaccc gattccatcc aagcattcac caatatggaa
    12061 gatgcagtaa aagacgcaga tgcggtgcta ttcgtcattc caacgaaagc aatacgttct
    12121 gttgcaaaag aactagtgcc taaattgaat acaaagccag tgatcatcca tgcaagtaaa
    12181 ggattagaac agggaacaca taaaaggatc tctgacatct tgttagagga aatcccagca
    12241 gaaaaaagaa aagctgttgt tgttctttct ggaccaagcc atgcagaaga agtagcgaaa
    12301 catgacatca ctacgatcac agctgcaagt acagatgcaa aagcagcaaa atatgtacaa
    12361 gagctgttca tgaataatta ttttcggatc tataccaatc ctgatgtgat cggtgtagag
    12421 atgggggcag ctttgaaaaa tattattgct attggtgctg gcgctattca cggcttaggg
    12481 tttggcgata atgcaaaggc agcaatcatg acgcgcggat tagctgagat cagccgttta
    12541 ggtgtagcaa tgggtgccaa cccgctgacc tttatcggat taagcggagt aggcgatcta
    12601 gtcgttactt gcacaagtgt tcattctagg aactggcgcg caggcaactt gttaggacaa
    12661 gggcagcccc tagaagaggt gctagccaat atgggaatgg tcgttgaagg tgtgaatact
    12721 actcaagcag cgatggaact agcccaaata atggaaatcg aaatgcccat cactcaaaca
    12781 atctatgaag tgctgtatga acaaaaagaa atcaaaacag ccgcaaaaga aatcatgtta
    12841 cgtgatggga aaaaagaaaa tgaattctca ttaacaattt tttgaaggag cgaatgtata
    12901 tgaaagtcaa aaaagcagtt attccagcgg caggattagg aacaagattt ttaccagcaa
    12961 caaaagctat ggcaaaggaa atgttgccaa ttgttgataa accaacaatc cagttcatcg
    13021 ttgaagaagc gttgaaatca ggaatcgaag acatcttgat cgttacagga aaagctaaac
    13081 gaccaatcga agatcacttt gattcaaata ttgaattaga aacaaaccta agtgaaaaag
    13141 gaaagaccga attattgaaa ttagtagaag aaacgacaga tgtcaacttg catttcatcc
    13201 gtcaatctca tccaaagggt ttaggacatg ccgttcttca agcaagagcc tttattggaa
    13261 atgaaccgtt tgtcgttatg ctaggcgatg atatcatgga agatgaagta ccattgacac
    13321 agcaattaat cgatgattac gaacgtactc atgcttctac gatcgccgtt atgcaagttc
    13381 ctcatgatga aacttcaaaa tacggaatta tcaatccagg agaagtattg gaagatgggt
    13441 tatacaatgt caaaaacttt gttgaaaaac cagatccaag caaagcacca agtgatttag
    13501 ctattattgg acgttactta ttgactccag aaatttttga tgtattgtcc gagcaacaac
    13561 caggagccgg aaatgaaatc caactaactg acgcaatcga tacattgaat aaaacacaac
    13621 gcgtatttgc ccgtaaattc acaggtaaac gttacgatgt cggtgacaaa tttggtttta
    13681 tgaaaacaag tattgaatat ggtctaacac atccagaagt aggtgcacct ttacgtgagt
    13741 atatcctgtc tttaggtaaa aaattagaag cagaaggaag caagatcgat cataatccaa
    13801 aaggaataac agaaatcaaa gaagaaaaga aaaaataacc cttttttcta acgagcttca
    13861 gctgctaatt gaataaaaag tagttgaagc tcgtttttat ttgtttaaaa aacacgaaaa
    13921 tgctatcctt atagaaaaga gagacaagaa gggagtttga tcatgtcagg aggagaaatt
    13981 gcaggattga tcgcagcaat cgcctttgcg atacttgtcg tttttatcgt tcgtgtgttg
    14041 ttgcaagttt ctaaaacttt agaaaaagta gatcagactg tagcagaagc aaatacgacg
    14101 atcgaaatcg tcacaaaaga cgtagatctt ttgtctagac aagtagaagg cttgctggtc
    14161 aaaagtaatg aactgctctt ggatatcaat ggaaaagtcg caacgatcga tccattgttc
    14221 acagcagttg ctgatttaag tgaaagtgta tcagaactta atcattctag taaaaacatc
    14281 gcgacgaaag tcggcggaat cggtaaaacg acagctaaag cgacggttgc cggaaaagtc
    14341 ggtgggacag cgatgaaatt ttttaaaaac aaaaaacata ctgaaacgac aggaggaaaa
    14401 taaaaatggc taaaaaaggt ggatttttat taggtgcagt agtcggagga acagccgcag
    14461 caatcgccgc gttattgtta gcaccaaaat ctggtaaaga acttcgggat gaattagctg
    14521 cccaagcgga tgatttgatg gatatggctt cagattacac agatatggcg aaagaaaaat
    14581 cgactgagtt aacaggtatt gcaaaagaaa aagcgcaaga tctttctcaa caagcaggtg
    14641 atctagcagg tagtgtcaaa caaaaagtaa gtgaatcagc agatgaagca ggaagtgaat
    14701 ctgacgatat cttgttaaat cttaaagaac aatcagctga cttatctaat cgtttcaaaa
    14761 aaacggcaga agatatgaaa aaacctgcag atgaattagg aagtattgct aaagatactt
    14821 cagaagatat catgattgat gtaaaagacg ctgctggtaa agcaggagat actgtcaatg
    14881 aaggaatgtc agaagcaaaa ccagttgcag atgacacaaa agaaatgatg aaaatggcaa
    14941 aagaagatgc gaaagaagct aaaaaagaaa taaaagatac aatgacagat tcatctacaa
    15001 atcactaaaa tcactaaaat cacaaattta tagaataaaa aggtgggagc ggactagctc
    15061 tcaccttttt tactcttatt tttctaaaac tagtaattct tttggtgtag tcgtgaatgg
    15121 cacacagcca tcgcttgtca catatacaca gtcttcgata cgtaccccta ctttttcagg
    15181 aatatagatt cctggttcaa gagagaagca catcccttct tcgattacta agtcatttcc
    15241 ttctacaagg gaagggaatt cgtggacggt tgttccgatc ccgtgaccta aacggtgatt
    15301 gaaatattcg ccgtagccat agctgttgat cacgtttcgt gcgatttgat caagttcacc
    15361 tgctgtcaca cctggacgga cggcttcttg tgcggcaagc tgtgcttcaa gagtgatgtt
    15421 gtatattttt tcttggaagt cggttggttt ttgataagca attgtacgag tagcatcact
    15481 gcaatagcca ttccaaacaa cgccaagatc gaataacaca agttcattcg gtgaaatctt
    15541 cgtgtttcct ggtgtaccat gagggcttgc agcatttgct cctgcaagga ctaatgtgtc
    15601 gaaagacatt tgtgaaacac cttgtttttt caattggtat tcgatttctg caatgatcgc
    15661 ttgttcagtc acgccttctt tcactgcttt aaagccaatc tcaaatgcaa catcggccca
    15721 tttaccagct tcgatcagtt tgtctttttc ctctagagtt ttgatcagtt gtagacgttg
    15781 gatgatggaa gctagatcag tgtaagaatc cgttcccgac aagatatcag agatagcatc
    15841 gaagcgctcg acagtcaagg cgttcttttc aatccctaca ttgccaggct gaccatagcg
    15901 agcacgaagt tcttttccga tgatctccca agggttttcg ctgtctaaat agccacggac
    15961 atcatacgcc caactactgt tttgagcatc ttcaacttcc aaagcaggag tgaaaaggaa
    16021 aggatcttgt tcaagaggga taaaaagagc tagtacacgc tcatgaggat cactttcgta
    16081 tccggaaaag taagcgatgt ggccagggtc gctgatataa gccaattcga cttggtgttg
    16141 tgccatccat tgacgtagtt cattgatttt tacgttattc atttggcaac ctactttcta
    16201 aattaatttt gtattcaata attgtaccac tgatttatta aaagaccaac gttctaaaaa
    16261 ctgaaaaaag taaaaatttt catgaaaaca gagataaaac ggttgcaatt cttgagatta
    16321 tttgatattc tattttagaa atgtaagacg tattttcaga aaacatataa aatatgaata
    16381 ggagaacaca aatggaaaag caaacaatca ctatctatga tgtagcgaga gaggcaaatg
    16441 tttcgatggc tactgtttca cgtgtcgtaa atggcaatcc aaacgtcaaa ccagcaacaa
    16501 gaaaaaaagt cttggaagtt atcgatcgct tggattatcg tccaaacgca gttgcgcgtg
    16561 gcttggcaag taagaaaaca acaacagttg gggtaatcat ccctgatgtc agcaatatgt
    16621 tctttgcttc tttagcacgt ggtatcgatg atgtggcaac aatgtacaaa tacaatatta
    16681 tcttagcaaa ttcagatggt aacgaccaaa aagaagtcaa tgtattgaat aacttactgg
    16741 ctaagcaagt ggacggcgtg atcttcatgg gtcaccatat cacagacgaa atccgtggag
    16801 aattttcacg ttcaaaaact cctgttgtat tagctggctc tattgatcca gatgaacaag
    16861 taggtagcgt aaacattgat tatgtatcag caacaaaaga tgcagtaaac aaattggcaa
    16921 aaagcggcaa taaaaaaatc gcttttgtca gtggtgcgtt gatcgatcct atcaatggac
    16981 aaaatcgtct gaaaggctat aaagaagcat taagcgaaaa taacctacca tatagcgaag
    17041 gattgatctt tgaagcacaa tacaacttca aagatggtct atctctagtc gatcggatcc
    17101 ataatagcgg tgcaacagct gtctatgttt cagatgatga attagccatc ggtattttgg
    17161 atggtttatt agaccgtggc gtgaaagtac cggaagaatt cgaaatcatc acaagtaaca
    17221 attcactact gacagaagta gctcgtcctc gtttaacaag tatcacacaa ccattatacg
    17281 atttaggggc ggtatctatg cgcctattga cgaaaatgat gaataaagaa gaagtcgaag
    17341 aaaaaacgat tatcttgcct tatggaatcg aagaaaaagg atctacaaaa taataaaagg
    17401 acaaccattc agaggttgtg agcctgccgt ttaactctgc gttacaagga gcaatttcaa
    17461 aaaacagcat ttcatgtttg atgaatattg tgactcgtga ttcggagttg gcaggagaac
    17521 accgtttatt cgggaaaagg ggttatgaca aaattttgtt taatcctttt tttttgtctt
    17581 aaaaaagaga gaaagtggtt gaaattacat ttatcgtata taaaacaatc cgtgtataga
    17641 tgctgcatta tgttcaccac catcaaaaaa ataagggact gtgacaatac tattgtcaca
    17701 gtcccttatt actgtgtatt ctattaaatc aatcatcttt ctcatctgat tctttggttg
    17761 cttgattcgc tcttgcataa gctgaagcgg tgttccagta atctgtatag ttttcatcgg
    17821 taccgccaac gccaaattta aaggcgcttt tcttcggtcc atttttcgcc catagcgatt
    17881 caactgtttt tcctggtgtt tggatggatt ttttattgac agtaactttt ccaggtgttt
    17941 gaccagtaaa tccagatact tttttcttca tcacatcaga tgataattcg aatttctcat
    18001 cagtgccaaa aatgtcaggt tttgcttggt aagcttgatt gatcagattg gcaagataat
    18061 tagccgaacg tatccttgct ttgctgtcca ttccagtatt gtcatcgtgc cctgcccagc
    18121 tactgatggt aatagacgga gtagagacaa ttagccatga atctcggtat tcattggtag
    18181 aaccagtttt tccgacccaa tctgcttttc ctagatttcc gtttaagctt gagatggcat
    18241 ctttgaatgg tgtagtgatt tttgcattga tcacactgcg catcatgtca ttcatgatcg
    18301 atgccgttgc ttccgaatag atccggacag agttgctttc gtgtttataa attacatttc
    18361 ctgcgttatc tgtgatggaa tcgatgatat agccttcttc atagacgccg ccgtttgcta
    18421 aagcctgaaa tccattggtc tgttgaagcg tcgtgacatc tactgtacct aaaggagcag
    18481 attcaacgcc ccaattgtca tttgccgggt agttcatctt tgccaagtag tgctcgtaag
    18541 catattgttt cgatccaccg gaattcaaca catcttgata taagtgataa gccgggatat
    18601 tgttggacca ttctagtgac tcacggacgg tttggaaggt gttcgatcct tcgttagtag
    18661 cattgacgat tttctcgcca gcattttctc cttcttgcca agttgttgca taatctgata
    18721 cacgggattc tgatccgata agccccatgt cgattgcagg tccataaaca agaactggtt
    18781 tgattgatga gccagcttga cgttctgtat cgaaggcatg gttattttga ttctgactgt
    18841 aattccgtcc gccaacgaat ccgtagatgc ggccagttct gttatccatc aatacatttc
    18901 cggtttctac ttgactgcca gcaccatcat ctaacatgta tccataattt tctacgccat
    18961 tttgcaatgc agcatagaca tctttatcga ttgtcgaatg gattgtatat cctttatttt
    19021 ggatcgtttg ctgtgccttt tgataataag cgtcactgac cgatgaatca gacatatcag
    19081 cgttgtcttt ttcagccaat tgttgtgcaa tgattcttgt tgcttcattc atcacagtat
    19141 aatacaagaa ttctctgtca gtctgttctg cgatttgttg tggtagaaag tcttttgtaa
    19201 ggtcataggc ttttgcttct tcgtattgtt ctttcgtaat ttggccttca cgatacatac
    19261 tgaataaaac aaaatctttt ctggcaaggc cggcagaaag atcttctttt agcgcacctg
    19321 tgttcgtgta tggactatac gtgatcgggc tttggggtaa tccagcgata taagcagctt
    19381 gcggcagcgt cacatctttt gcatctacac caaagattcc tcttgcagct tcctggactc
    19441 cagcaatgtt ttgtccctta ttattacgac caaatggtga gacattcaaa tacgtagcaa
    19501 tgatctcatc ttttgaaaag tatttttcta cttgtgcaga aagaatgatc tcgtttgctt
    19561 tacgtttgaa tgttgtttca tctgtaagga tctgctgttt gactaattgc tgtgtcaaag
    19621 ttgatccacc agaagagcca ataccagtag cttcagatac taatgctcgt aaaacagctt
    19681 taggaacgta accttggtgt ttatcgaagt attcatcctc tgtgctgata attgcagttt
    19741 tcaataaagg tgaaatttta tcagatgaaa tagtcgtgcg catcagatct gtctggattt
    19801 ttgatatctc tgtattatct gcataaacaa tctttgacgt ttctgtaatg tttcctagtt
    19861 ctgtctgcag tgctttttta gtcggcaatt gtgtgtcttc gacgagatag gcaaaatagc
    19921 cagcgcctat tccaatacct agagctgcga acatcaaaag aagaacagta ataaaaacag
    19981 tcaatgattg aaaaactcta aaaataatct taggaacaaa ccatttttct ttttttcttc
    20041 tattctgttt ttttcgagta tttttgcgtg gcaaaagggt cacctcaact aatggtttta
    20101 tttcgctaat tatagcaaat aatagaacaa aaaaacagac aaggctgttt ttggcaggaa
    20161 tttctttttt aaaagagtta aaacgtgaga aaatgatctt ttttataaaa gagttcttaa
    20221 gcttttcttt agagaaaatt tcagttacta tggagaacag ggcaaagaaa aatcaatcat
    20281 tttctttgta tatcatctta ccaatatata ggaattgtta gtaacaattg cctttcaagg
    20341 aggaatccat ccatgttatt aggctcacac gtcagcatga gcggaaagaa aatgctgtta
    20401 ggagcagcag aagaagcagc cagttatgga tcttcgactt ttatgatcta tacaggcgcc
    20461 cctcaaaaca ctagaagaaa agccatcgaa gagatgaata ttgatgccgg aaaagcgtac
    20521 atggcagaaa acggtctaac aaatatcgtc gttcatgccc cttatatcat taatttgggg
    20581 aatacgatca aaccagaaat gtttcctttt gccgtccaat ttctgagaga agagattgtt
    20641 cgtgcggaag ctttaggcgc tactcaaatt acacttcacc caggtgctca tgtaggggca
    20701 ggaacagaag ccggaattgc tagaattatt gaaggcttga atgaagtttt gacaaaagaa
    20761 cagacggcac aaatcgcttt ggagacaatg gcaggtaaag gtacggaaat cggtcgaaca
    20821 tttgaggaat tagcgcaaat catcgatgga gtaaaattga atgaaaaatt atccgttact
    20881 tttgacacct gtcatacgaa tgatgccggt tataatatta aagaagactt ttcaggtgta
    20941 atggaagagt ttgataagat catcgggtta gatcggctta aagtactcca tatcaatgat
    21001 tctaaaaatc cgcaaggttc gcataaagac cggcacgcca atattggttt tggaacaatc
    21061 ggattcgaca cgctgaataa gatcgtccat gatccaatgt ttgacaatat ttcgaaaatc
    21121 ttggaaactc cctatgtcgg caaagataaa aagaaagctt acgcaccata tggaaaagaa
    21181 atcgccatgt tcaaagcaca aatattcgat ccagaagtgt ttcctgagtt attagcagaa
    21241 gaagtgatac tctaagattt tctatctgac agcagcaaaa aaggaccagg cacattgcct
    21301 tgtccttttt tgtttaagaa acaaaggagt gctttttgca gtctttttca tcatgcatct
    21361 tctcccccct ctttttaaat ttagacaaac agggtatact ggtagaaaag taaaaaaacg
    21421 aaaagtttca caagcaagtg gcaaaacgtg attggaagga cgaaaagatg ctgaacactt
    21481 taaaaccctt gatcaaccaa ctaaaaaaga aacatacata ctttattctt ggcatagccc
    21541 tattatttac aattattcgt ttgtatttat atagtaaagc aatctatgga acagacatgt
    21601 cggcaaccta tgatgaccag ctgctgattc gctatgctca aaatattttg gacggtaaat
    21661 ggctagggga gtatacgact acaacacttt ccaaaggaat ctcctacagc ttatttttag
    21721 tacttgcaca taaactgcat ctttcttatg cagttttgtt cggcatgttc agtatttttg
    21781 ccagtatatt ggttgcactc gcgtttaaac cgatcgtgaa gaacaagtat cttcttttaa
    21841 gtttctattt ttattttttg ttttctccga ttagttttac acatgaatat tccactcgaa
    21901 cgtatcgtaa tgccctcgtg attccggctg ttgagatcat tatcgcttgt ttactagcta
    21961 tttattaccg taaatatagt tccagcaaaa aaatgctgcc gtggttttta gggctagctg
    22021 tttcttttcc tttcttttgg ttcattcgcg aagattccat ctggcttctt ccttttactg
    22081 tagtcgcgtt aggcatcagt ttcgtccaga tcttgctagg acaagacttt tctttcaaag
    22141 aagcaaagaa gttaaaagaa cgaccatttc tcctttcgaa acaagaatgg ctgaaacttt
    22201 cattgttttt actccctcta gtcggtttct ccatcgtcta tcagtggatc gaatataaaa
    22261 acgagacaac atatagtatt gccgccatca atgaccgttc gagtggcgaa tttggacgtt
    22321 tgatgaaaca gctgatccga atagatgacg aaacgaactt aaataaagaa aacagtgaga
    22381 tatgggtctc gcatgctgca ttaaataaag cattagacgc atctcctaca tttcatgctg
    22441 tttctactcg tattgacgaa ttgtatacga ctcatgcgtg gacccaaggt ggaaaagtaa
    22501 aagaattacc aggagatatc attttttggg cattgcgaga tgtggtaagc gaatctggtt
    22561 attaccaaaa caatgcaaaa gaaaccaatg atttttggaa aaaagtcaat caagaattag
    22621 aagaagccta tcaaaaggga actatcaaaa agaaaaaaga attctatctg atgggatcag
    22681 gggatggaaa gacgatgaat gatctgccaa tcgtatttgc ttttctgaaa tcaggatatg
    22741 cctatgcatg gcgatacaaa gatttccatc aaggggggaa ttatagtttc ggtacgcaag
    22801 aacaagtcga acaggcccaa gaattattga atcttccact aatggataat tggatcgata
    22861 gcagtgatcc gagaacacct gatttccagt tgacaaaaac agcaaaagtc gcgaatgtca
    22921 tgataaaaat ttaccaagtt ctggccattc ccgtgctgat tgcttcaata gcagggatat
    22981 tacttatgat actaggaacc gcatttgcaa aagctgatcg tgcctattat agttcctctt
    23041 tgattgtagt caccggatta atactgacac aattggtttt tcttttcggc gtctcgtggt
    23101 tttgttctta tgcgccagaa aaaaaggatt attttttaag tgtctacact ggcgcaggcg
    23161 tgccattaac gcaattcatc gtagtaacaa gcatgctagt tttcagtaaa ttgaatctga
    23221 aaaaagcttt atcttccaaa taaaaacata ctaagattag aggagaacat cattgacgaa
    23281 tgatgttctc ctctattttc tatttttaga ttatagtgaa gagtgaaaga aagttcgaac
    23341 aaagccttga ggactatgag tccctatcaa aaaccgaact tctttactgt cattttagaa
    23401 tcaggtttac gtatgttggg gccatgagcc cgtgtatagg aaattggaat cgaaatgaag
    23461 cagtaaagtt ctttcaaaac ttaaaaagga ggaaaacaaa tgccttacat tcttgcatta
    23521 gatgtttcaa tgggacacag ctacagtgtc ctatacaaag acgacacctg cttatttgaa
    23581 gacgagatcg aacacactca aagaggattt catgcattac tcgaagagat ccagcacttg
    23641 cctgaacgtc cattgattgt ttttgaagca acggggattt attccagacc agtcgaaaag
    23701 ttctttcaag atcatcactt ttcttactgt ttgttaaacc cgttagaagc gaaaaaacag
    23761 atggaagaaa acaccttatg cagttggaaa acagataaat cggatgccca
//
LOCUS       DASHHB010000038        23540 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.039, whole genome shotgun sequence.
ACCESSION   DASHHB010000038 DASHHB010000000
VERSION     DASHHB010000038.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23540)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23540)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23540
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.039"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1166)
                     /locus_tag="U2K71_001768"
     CDS             complement(<1..1166)
                     /locus_tag="U2K71_001768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEM7315848.1"
                     /translation="MSNDIQPEIRFPGFTEAWEQRKFECLLDKKDGVRRGPFGSALKK
                     EFFVSNSNFVVYEQQNAIYDNYETRYKITEKKYNELIKFKLEPGDFIMSGAGTIGRIS
                     RVPKQIKPGVFNQALIRLRINKEITDSEYFIQFIRADFMQRKLTGANPGSAITNLVPM
                     SEVKKWIVQFPILEEQKKIGNFFKQLDDTIALQQRKLDLLKETKKGFLQKMFPKNGAK
                     VPEIRFPGFTEDWEQRKFKEFSKKTGKKNTKDLDFPAYSVSNKAGLISQTEQFDGSRL
                     DDLEKTNYKFVEPNEFAYNPARVNVGSIAFNNLGMTVIVSSLYVVVKMSEDLDNEFIL
                     QFIKSPTFIKEVKRNTEGSVREYLFYENFANIKFPFTRNKEEQQKIGAFFKQLD"
     gene            complement(1159..2751)
                     /locus_tag="U2K71_001769"
     CDS             complement(1159..2751)
                     /locus_tag="U2K71_001769"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEM7315849.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLANFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHE"
     gene            complement(3261..3986)
                     /locus_tag="U2K71_001770"
     CDS             complement(3261..3986)
                     /locus_tag="U2K71_001770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315850.1"
                     /translation="MKKKKLLWICLAILLIIGGIEIFTTYRLKTNQPKQYVSDMLNII
                     EEIDSEMKAFSKTSMDLGERYDFKGFMEDKMDEVSSREIPEGTPKAVRDTKDTLLEAI
                     DNVNTALLTNDVKLLEQGQLCFSIAQAIYTEFLDGNIKEKSLNEKLNSFVETYTAIPP
                     TWKLVNEDKGITINTDTEISTDAQSLSDLLANLTGISNAVTEKVGKDIPLRLLENGKK
                     DYSVVFKNGQVTDYNEVFKKVIE"
     gene            complement(4140..4466)
                     /locus_tag="U2K71_001771"
     CDS             complement(4140..4466)
                     /locus_tag="U2K71_001771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315851.1"
                     /translation="MNKVNMNKKFVIIGIVVLAIIGIIFSRMTGSSGLEKSSVPLVTQ
                     ILQEQYNLNRTCDDVTITHDNGDNTYRAKATLDNGSAININVEYYPKKDRVYVEIPYT
                     EVLMLN"
     gene            complement(4503..4868)
                     /locus_tag="U2K71_001772"
     CDS             complement(4503..4868)
                     /locus_tag="U2K71_001772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HEM7315852.1"
                     /translation="MYCRKCGKEITNESKYCQFCGALQETDETTGTDTVKKSTEQTIN
                     EMKEQIVHGQETGKLYLIIGWISMVVSLAFIPVVFGAVAVIMGYLYREKDEQMGTILM
                     IAGVAGAIFGVLLGMSMSY"
     gene            complement(4905..5120)
                     /locus_tag="U2K71_001773"
     CDS             complement(4905..5120)
                     /locus_tag="U2K71_001773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315853.1"
                     /translation="MKKVSNLSRYRKEKGLSQTELAKKMNVTQQCVSSWQTGRTIPKP
                     YQMKMLSEILSVPINELFSDVFNKVNS"
     gene            5249..5584
                     /locus_tag="U2K71_001774"
     CDS             5249..5584
                     /locus_tag="U2K71_001774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315854.1"
                     /translation="MKQKQYAKVIREKRKALGWTQKELAKKIFSTQQAVARWENSVTE
                     PNLDSLTALSRALGTPVSHFLDNVDIDYEEEFLALYRSLSTEDAIRTIDYMKLLKRQE
                     NERNQLLKD"
     gene            complement(5660..7657)
                     /locus_tag="U2K71_001775"
     CDS             complement(5660..7657)
                     /locus_tag="U2K71_001775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315855.1"
                     /translation="METSMINIPIYVDKEKLLEKPVTKKEKTDVRDRCLDNYREVTRD
                     EFVEILNSSQSFIPSKTKNKGNDKESFVETRVIILDVDNTVKDEKGKVVDLSKDDSRY
                     LSIEKVLSINLVHNSAFAIQKSIRYSENLEKYKIVFLLKEAITDYGKMVAVYEYLKKN
                     IPGCDDNVNASNRMFFGGYKSDAVIIINEDNMLDITELPIDFTMTCSKNCQSDSGREY
                     ELVNETDFVKLIKNDDKEEMKQWFKDCLFDSSDMSFNEIYERLLTIDMNKLLKSSKNL
                     RCLFHNDHNPSASIFTSKTGHSIFYCHSSNCDISENFIGVVMLILKKDSRVETFNWLL
                     ENLDLYPTHFRKLKEESKVLFDTLESMKDKLFRTIRNYLEEMRRVYDVLLSDVNFFDD
                     SKESVTCILSGEQLAKKMSSYYGKTYDIDKCNKLLSLMTFIGLIKKLDDEDVPLKMLE
                     YLNRLKEEKALGNFKHKRSNVYRLDVFNATSVIQKLNEDILPLLEKFHFTYQYFSYEW
                     VKICFNEKEAKRVFPQNSRSELSKEKQLILDEAHEVLQGLSWQGTYLMTEKELINTVC
                     ERLRNVRDNSVKNALIENRGYFVKQGFTLERASKDIKKLYNVKASSSFLVYLAKGAGI
                     DEVIKELILSGVELVPIYQFTSVTRDDGRRVRNRRDPIILN"
     gene            complement(7755..8030)
                     /locus_tag="U2K71_001776"
     CDS             complement(7755..8030)
                     /locus_tag="U2K71_001776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315856.1"
                     /translation="MSLQIIDSIVSIQVEQQVKEIITVLKPMLEDIISGVTLEYMDYQ
                     QASEYLGVSVGTIRKYVSQYGLPVIKIDTVIRFKKSDIDSFMERYKG"
     gene            8262..8996
                     /locus_tag="U2K71_001777"
     CDS             8262..8996
                     /locus_tag="U2K71_001777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315857.1"
                     /translation="MIKRPPINYLERKKILGTKIKAIRKSKKLTQPAFGLMINNGQLI
                     DKKTIYEWEKGTYLPIPERLSRIADLGNMSIEELVCGNVEEYILGIILYRDSIVLDGI
                     TFPDKNLFQHLRQQFPPVHSNLDTWLDRYSKLEPEMQEFIANKTCNKVKNEKISLFNI
                     LKIEELFINAIVEEFDNNILFLTSSIEELLERMVDEWLPIQLKDMSYPEEAVREITDN
                     INKLEQTISSIGKKYTKKKMKGGDTI"
     gene            9035..10177
                     /locus_tag="U2K71_001778"
     CDS             9035..10177
                     /locus_tag="U2K71_001778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM7315858.1"
                     /translation="MAKISKYKKKNGQVAWMYKGHIATDPRTGEKINTTRRGFSSKQE
                     AQKDYDEYRHQILYGVKKKAPDMTFEDLYNEWITHQRTSVKASTIAISVRYANNQILP
                     AFGKLKLSNISVPYCQKVVDEWHSKYESYDYMRKQTAQILRYGVAMQYIDNNPMEKTL
                     LPRKKEYEKNRKFYSKEELNNLLDAFKDFGNMKQYAFFRLLAYTGMRKSEVLALQWKD
                     IDTFNKELHVNKTLAVDEFGKVIIQSPKTRASRRVISLDTETLSILNNWKLQQKEEYL
                     KLGYNTSSKEQHVFTTVKNTLYIPNTVNDWLRYILKKYNLPRITPHGFRHTHASLLLE
                     AGESVKVVQQRLGHENSKVTLDIYAHITNNAPKKTGQDFADMMAHQ"
     gene            complement(10805..11290)
                     /locus_tag="U2K71_001779"
     CDS             complement(10805..11290)
                     /locus_tag="U2K71_001779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIR domain-containing protein"
                     /protein_id="HEM7315859.1"
                     /translation="MVRRVFFSFHYADVTRAMVVRNSWTLRGTKEAGFVDKAEFEKVK
                     RNGDIAIKNWIDNQLIGTSVTVVLIGKETLDREYVKYEIQESYKRGNAILAVNIGKIK
                     DFNQMTTTSQSVIKIIGQKPDGEYLWFDEIIDGQYDYISDDGYNNLGTWIDSAAYKKG
                     K"
     gene            complement(11292..12728)
                     /locus_tag="U2K71_001780"
     CDS             complement(11292..12728)
                     /locus_tag="U2K71_001780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIR2 family protein"
                     /protein_id="HEM7315860.1"
                     /translation="MDKKVLIKRFSEAIEKGNAAIFAGAGLSMSQGYVSWPELLNDPA
                     TEIGLDSKKETDLVTLAQYYKNENGGSRGILNQILMDNFGEELEISENHRILASLPIE
                     TYWTTNYDHLIEKSIREAYKNPQVKKNYTQLATTNPNVDTIVYKMHGDIDDVSSTVIT
                     RDDYEKYDDDSYALFKETLKGDLLTKTFLFLGFSFTDPNLERILSDIRWVLRENQRPH
                     YCIMRKILKENFVDSEDFFDQERYNYELTKRRLQINDLSRFSINVVEVDDYSEITDIL
                     KSIRKKYLRKTIFISGSAVDYTPFTSESKGLKFVEKLAYRLSESGYRIVSGYGLGIGN
                     SIVSGVLKQRRNLRKNNIQDVLSLRPLPLDMPHEWRRYRENIIAESGISIFVFGNKLE
                     SGEIVTADGMIEEFELSVQNENIVVPIGFTKGASKVLFDKIQENFSDYFDDSLKSKFQ
                     ALEKLDTEGEVDKKVEQIVSLINSIQED"
     gene            complement(12761..13612)
                     /locus_tag="U2K71_001781"
     CDS             complement(12761..13612)
                     /locus_tag="U2K71_001781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toll/interleukin-1 receptor domain-containing
                     protein"
                     /protein_id="HEM7315861.1"
                     /translation="MYKAYELDFTNADLNAFSSSSQSYTHVVNQIDHNQKSINKRIEN
                     LFESENDLVDYYSKDSKILDADEIIKDWFPTIKADIFISHSHADEKLAIRFASWLFEN
                     FGLTAFIDSSVWGYSSDLLKKIDQKYCYKEQTKTYDYDKRNVTTSHVHMMLSTALNNM
                     IDSTECLFFLNTPNSISLSNEITNEQKFTYSPWLYSELTTASIVEKKNPRLESNPQMS
                     TEDVRSIIKHYSSQENLQIKYSLNLSELTYLRKADLKKWALDAVECTKGSNTLDILYK
                     NTIFTNL"
     gene            complement(13768..14013)
                     /locus_tag="U2K71_001782"
     CDS             complement(13768..14013)
                     /locus_tag="U2K71_001782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315862.1"
                     /translation="MLFLKEEKIIDKENVIGSNIRRVRLEKGIGQTELIRDLQLRKIA
                     ITRETLVKIEGGRQHIKLDQLKAIKDILQVSYEDLLQ"
     gene            14118..14891
                     /locus_tag="U2K71_001783"
     CDS             14118..14891
                     /locus_tag="U2K71_001783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315863.1"
                     /translation="MKKFAIILTCALLIGTISGCTTSDTSSTSTETSTTTSEKNYPIH
                     FFPVPDGFEAYYHDKKLDVPLSETESTEKILPIEIERNQDDIKLKADLPLLNSTQIDR
                     HENIKVNIEKGALAQLYTEKVEQFFEAGANKDFSKISDYSDNFFNETQDEITNSTFMA
                     EDMYNMKLKKIKSYDYPLNLTLNKGILSFVLAGYVDYEYTADYLETPLQHDNNDINFR
                     FIYNSKSKTWLVDQLSTINTGFHELPKPDNETALVIKTF"
     gene            complement(14944..15258)
                     /locus_tag="U2K71_001784"
     CDS             complement(14944..15258)
                     /locus_tag="U2K71_001784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315864.1"
                     /translation="MADENKVVPLETLVINKEEFEQLFLDFFVQYDRIYDFSSSLDLF
                     ELEAEIIENSREENGKIIISVEKFNELFSETFEIDTDYYQPQEVFQSFRNQIIRSGKT
                     VN"
     gene            complement(16777..16995)
                     /locus_tag="U2K71_001785"
     CDS             complement(16777..16995)
                     /locus_tag="U2K71_001785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315865.1"
                     /translation="MKLILKISTHDFLELDELCESVKNATNEKITVTNYIELMIQEEL
                     KMKHEIKNNLKYCYLEKSEEIIHELNGF"
     gene            complement(17010..19637)
                     /locus_tag="U2K71_001786"
     CDS             complement(17010..19637)
                     /locus_tag="U2K71_001786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315866.1"
                     /translation="MKAIKEKIVSRKINWLALSVLVLMISVVFTSNIVEASIFNWFDD
                     TKSQTEFLNNSLYRPYLKKYNGDFLSQFGIWLGWAVVKGMFTVTDSIQNMIPDVLDLF
                     NFIESTGLNNVYQSVINTIVVGLMILSLMFVGYKMITGKGTIDLRSVGMNIVMSVALI
                     LLMPTMISSGIQFAKIFYNDATTITNSDDGVAWSLIKQGVTDLAYINKTDQYSSIDKT
                     EDRNKLTRKNFQQTDLTQVLTDKVIDKLEKENPAADNLRYELVENSNNEFVATKFSDN
                     FLSTFSDSLKSGYYRYQANLWGISIGLTALAIAYVFSAFVIITAILELAFKRVLGVLV
                     FATDIETGQRSKVVLSDILQCYLTVGFQGFGLSMFAMFINFLNAGQGISTNIFIKTIA
                     YICAVFVLIKGSGTVMRYFGVDIGLKEGYGQLASAFGMGAMLFRKGSTGFNRAKGSGN
                     GNGSDSGEGEDRKPEKNFGETLSKKAGKTGRALGYAHERGLSGLASDGATMASERAIK
                     PFKSMRDMANDTKNKFKEGLDDGTVSAINKNSKPMLAKNKEDETGKYADSMPTRLSDR
                     KDGAKVENADRIMSSSERMREAMKNNAESNNNPVSAIQQKVQQDIEERKNAMHGQAKS
                     AEELINQKRQEAKYTPEAMNREEMLRKRVEGRTGANTDEKEALTKERIQEAKNSNTGL
                     EKLVKENLQNSTSSQGGRSVDVRENIQGSLNGHVGRNVDVRENLQSSSNGQGTKTIDV
                     RENVQNSTSSQGFKSMDVRENVQNSTSSQGFKSMDVRENVQSSTSSQGFKTVDVRENV
                     QKDSGEMKEKTQKINIVEDRKSAKKFDSNHETVIIDSEIRENDKGSKPRRRFTYEDNE
                     LFRDTLNDPNPLFDNLLKK"
     gene            complement(19624..19842)
                     /locus_tag="U2K71_001787"
     CDS             complement(19624..19842)
                     /locus_tag="U2K71_001787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315867.1"
                     /translation="MIKNRLIDQMWCLDQNTDTLELIESICFNTVVLDLRAENDNCVT
                     HIIINQKMAQQLSESLRKWAEGENYESN"
     gene            complement(19839..20714)
                     /locus_tag="U2K71_001788"
     CDS             complement(19839..20714)
                     /locus_tag="U2K71_001788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315868.1"
                     /translation="MNAIVILAEITYWYRPKKIFNDDGQLLGYQKKFLEDILQKSYKQ
                     LSKKTGLSERAIKDAIVYLEKKGIIKRVFRNIYYEKRAVTLSNVMYIVLNVHKLIEIT
                     FPTLDCEKKEYMPKIRQRGIEKKRYVSLKKGRGMYKFVYTYTKTTSQNISKTTLVMEE
                     EDINIKRIYSKISVLYDANNEDGRKLFQVYHYLRNANVDTEDIRKIIEYLSENTQYLI
                     AECIIKQHEACFFQRKTEEGLYDYVTYFLNGLQKRYKSQFLENVEPIDQFKKAFEIKE
                     LPEITMHNWLKGEES"
     gene            complement(20979..21278)
                     /locus_tag="U2K71_001789"
     CDS             complement(20979..21278)
                     /locus_tag="U2K71_001789"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7315869.1"
                     /translation="MEAIKLMISEEQEEMFLKQFYQIATKAIEKAQENAGMTREYLNQ
                     KEMAEYLNVSENFLKNAVRNEGLPVVELGARKFYSKKAVNEFMFARMKRGENIGR"
     gene            21480..22277
                     /locus_tag="U2K71_001790"
     CDS             21480..22277
                     /locus_tag="U2K71_001790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315870.1"
                     /translation="MKPNPSEAGKRIKQLRLSCGFTMEELGRKIDNSPRATISNWERG
                     TNLPNPQKLKLLSTITNSTIDWIKWGTLEEYITSYLIDIGYELYIKDFPEIPHKVFKD
                     IQERYSDTFSLDKDYELLNPIIKNIFTKYYSKDFEIYLNEILANNINPLIEEYANQRH
                     SISKELFFKRANSKLQDKIRNGDFKYGEKERMIDSLKQLLDEMDLAYKDISKYSSVED
                     FFYKITKNQFETEKFISDLSKKYNFPYEKNSIIAKFLTDNHDKFKNN"
     gene            22328..23470
                     /locus_tag="U2K71_001791"
     CDS             22328..23470
                     /locus_tag="U2K71_001791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM7315871.1"
                     /translation="MATFFSYKLQNGEKYWGFETYLGKDPKTGKDIRTKRRRFKSKAE
                     AQTALNRLLVDFKEKQEFKINVTTFQELYELWIDNYRTRVKPSSVAVAQRFCKNHILP
                     AFGELRLDKISVSYCQKQVNEWHKTLKQYGFLRKTTAQIFKFGVAMEVCKDNPMSKTL
                     LPRKIEEEQPLKFYTKDQLQLFLQNTKENNSVKLYTFFRLLAYTGMRKSEALALQWED
                     IDYFNKTITIGKTIAQDEFNQVVLQVPKTKNSSRTIQLDDFTLKQLRIWQQEQMKIMI
                     LYGYNTNSPKQFLFTTNTNKLYYLQVVNDWLDWIYKKTPMEPQITPHGFRHTHCSLLF
                     ESGASIKEVQERLGHKDIKTTMNIYAHVTPQSVKKTGDRFAKFMGE"
BASE COUNT         7568 a         4333 c         3239 g         8400 t
ORIGIN      
        1 tcgtcgagtt gtttgaagaa agcgccgatt ttttgttgtt cctctttatt cctagtaaaa
       61 ggaaacttga tattagcgaa attctcatag aataagtatt ctcgtacgct tccttcagtg
      121 ttgcgcttga cctctttgat aaatgttggt gacttgataa actgaagtat gaactcatta
      181 tctaagtcct cgctcatttt cacaactaca taaagagagc taacaatgac cgtcatacca
      241 agattgttaa atgcaattga tcctacgttt actcttgctg gattataggc gaattcattt
      301 ggttcaacga atttataatt tgttttttct aaatcatcca acctactacc atcaaactgt
      361 tctgtttgag aaattaaacc tgctttatta cttaccgaat aggcagggaa gtctaaatct
      421 ttcgtgttct tttttccagt cttcttacta aactctttga acttacgctg ttcccaatct
      481 tccgtaaatc caggaaaccg aatttctggc acttttgctc cgtttttagg gaacattttt
      541 tgtaagaatc cttttttcgt ttcctttaat aaatctagct tacgttgttg aagagcgata
      601 gtgtcgtcca gttgtttgaa gaagttccca attttctttt gttcttctaa tattggaaat
      661 tgtactatcc attttttcac ttctgacatt ggtacgagat ttgtaattgc tgaacctgga
      721 ttagctcctg tgagttttct ttgcataaaa tctgcacgaa taaattgaat aaaatattct
      781 gaatctgtga tttctttatt tattctaagt ctaatcaaag cctgattgaa tacacccggt
      841 tttatttgct ttggtactct tgaaattctc ccaattgttc cagcgccact catgataaaa
      901 tcgcctggtt ctaatttaaa tttgattaat tcattatatt ttttttcagt aattttatat
      961 cttgtttcgt aattatcata aatagcattt tgttgttcat agacaacaaa attgctatta
     1021 gatacaaaga attctttctt taaggcactc ccaaatggtc ccctccttac tccatctttt
     1081 ttatcaagta agcattcaaa cttacgctgt tcccaagctt ccgtaaatcc tgggaaacga
     1141 atttctggtt gtatatcatt actcatgttt gaacacctcc agcgcacctt gtaaccacgc
     1201 ttcattttca ggtgttgttt gcagggaaga gatagcttct aaaagttctt tttctagcac
     1261 ttgctgttct tgtcgtatct tcttgatgtc atttccgaca tgaaccatat caattggttc
     1321 ttcttcttca aacgtatcta catagcgtgg aatatttaag ttatagtcat tttctttgat
     1381 ttcatcaaag tttgccaaat gagcgtattt ctcgacatct tttctttcag cataggtttc
     1441 gaggattttt tgaatgtttt cttccgataa tttattctga ttttttcctt tgacaaattc
     1501 tcgactagcg tcaatgaata atacgtctcg agtttggcgg ttctttttca atacaatcac
     1561 cgtcgttgga atcgatgtcc cgaagaacaa gttcgcaggc ataccgatca cagcataaat
     1621 actgccgtct tctagtaatt tttgacgaat gaccccttct gcagcgccac ggaataaaac
     1681 tccgtgtggc aagacgatgg ccatagtccc tgtttccttc aaatgataga aaccatgaag
     1741 aagaaacgca aaatctgctt tggattttgg cgctaatttt ccataacgat taaaccgaga
     1801 atcgtctaag aaggtcgtat ctgctgacca attagccgaa taaggtgggt tcattactac
     1861 tgcatcaaat gtgtaaggtt catctgttgg ccaatcctta ttcaacgtat ccccattgcg
     1921 gagattcatt tcttcggcat ctaccccatg taaaatcaag ttcatttttg ctagattgta
     1981 tgtcgttgta tttaattctt gcccatgata tttaacgtta tctgggtgag ttaagtagtt
     2041 acgcacattt aacatcaatg aacctgaacc catggttgga tcaaaaacac taaagaatcg
     2101 tcgttctttt tgatccaacg tcacgatttg tgccatcata tcagaaacca tatgaggcgt
     2161 gtaaaattct ccggctttct tccctgcttc tgaagcaaat tgactgatca gaaattcata
     2221 agcatccccg atcacgtccc catcgtgacc taaaacttcc acgtcattca atttcttaat
     2281 gacttcggta atcgtcacat ttcgttgttg ctcatctgtt cctagttttt ttgattggag
     2341 atccacatca tcaaataacc cattaaattg attgtaggta gatgccaatt gcacaaaggc
     2401 tttgtttaga tcattcaatt ggaacgttgc ttgttttgct tgatccgcta agacgttgaa
     2461 aagatactct ggcgaaatcg cataccccaa aatatcaacg attgttgcaa tcagatcttc
     2521 ttttgattct tcatctgaaa gtaattcgcg atacaacatc gtttgtttgc ttaccgtatc
     2581 atattcctct aaggattcat ctgctaaaag aaccacttgt tccaacaaac ggtcggataa
     2641 atatttatag aaaatcagtc ccaataaata atttttatat tctgacgcat ccattttgct
     2701 acggagatta tctgccgcac taaataattt ttgatttaat tctgcaccca aaaagataca
     2761 ctcctatctc ttatttaaag ttgctatata ccctcttatt ttagcatact cccttttaga
     2821 aaaagcaaaa aaaaaaagaa agaaacttat tcttgctaat aaactacgcg aaaggagtct
     2881 aacgttgttt gatataactt agactgatcg ttaacttatt ttttatattt ctacatagtt
     2941 atctttagaa ccatttcaga tacatatgaa acaattacat atgatttttt cataaaaaaa
     3001 cagtactgtc tagtagtata aaatatacaa atttcttaaa aattttttca ctaaaatcac
     3061 tacctgaaac agtctaaaac acttatatat caacgtttaa tgaaactatc tccgtttcat
     3121 ttcagataca aataaatata gtcgggctcc aaatccgaaa aaaaataccc ccagtagctt
     3181 atcctaagct actggagatg tataagtacg gtcatataac aacggggtag ggtaaataaa
     3241 tgaaaaagat ggaagaatca ttattcaata acttttttaa atacttcgtt gtaatcagtt
     3301 acctgtccgt tcttaaatac cacagaataa tcttttttcc cattttcaag taaccgaagt
     3361 ggaatatctt taccaacttt ctctgtcact gcattactga tacctgttag attagctaat
     3421 aaatctgata aagattgagc atctgtagaa atctctgtat cagtatttat cgtgattcct
     3481 ttatcttcat ttacaagttt ccaagtaggt ggtatagctg tgtatgtttc tacaaatgaa
     3541 ttaagttttt cattaagtga cttctctttt atatttccat ctaaaaactc tgtatatatg
     3601 gcttgagcta tactaaaaca aagctggcct tgttctaata attttacatc attagttagt
     3661 aaagctgtgt tcacgttatc aatcgcttca agaagagtat ctttggtgtc cctaacagct
     3721 ttaggtgtgc cttctggaat ttccctactt gatacctcgt ccattttatc ttccataaac
     3781 cccttaaaat catatctttc tcccaaatcc atagaagttt ttgagaaagc tttcatttct
     3841 gagtctattt cttcaataat atttaacatg tctgatacat attgtttagg ctgattcgtt
     3901 tttaatctat atgtggtaaa tatctcaatt cctccaatta tcaataaaat tgccaaacaa
     3961 atccataata attttttctt tttcattttt cctcctatac actgctgtta taacagtgac
     4021 taatgtaaag attttcccag caatcatcag actattattt acagggaatc aaaaaaactc
     4081 cttagtaagc ttacaccttt aggagttcct gtttgaacgg tatgcataat acatttaaat
     4141 caatttaaca ttaacacctc agtatatggt atctctacat atacacggtc tttttttgga
     4201 tagtactcga catttatatt gatagcactt ccattgtcta acgtagcttt agcccggtac
     4261 gtgttatcac cattatcatg agtgatcgtc acatcatcac atgtccgatt taagttatac
     4321 tgttcttgta aaatctgagt taccaatggt acagatgact tctctagtcc agatgaaccc
     4381 gtcattcttg aaaaaattat acctataata gccaatacaa cgatacctat aatgacaaac
     4441 ttcttattca tatttacttt attcaacttt ttatctccta tctccaaaat gattttctct
     4501 catcagtaac tcattgacat ccccaatagt acaccaaaga tggctccagc tacccctgct
     4561 atcatcaaaa tcgtacccat ttgttcatct ttctctcgat ataagtaacc cataataaca
     4621 gcaacagcac caaataccac aggaataaat gctaatgata ctaccataga aatccaacca
     4681 ataatcaaat acagcttccc tgtctcttgt ccatgtacga tttgttcctt catttcatta
     4741 attgtctgct cagtactttt ctttactgtg tctgttccgg ttgtctcatc tgtttcttgt
     4801 aatgctccgc aaaactggca atacttactt tcatttgtta tttctttacc acattttcta
     4861 caatacatgt cttctcctcc taaaatcatt caattgctta ttagctaact gtttacttta
     4921 ttaaatacat cagaaaacag ctcatttatt ggtacactta atatctcaga aagcattttc
     4981 atttgatagg gtttaggaat cgttctccct gtttgccacg aacttacaca ttgctgtgta
     5041 acattcattt ttttggctaa ctcagtttgg gaaagtcctt tttcttttct gtatcggctg
     5101 agattactta cttttttcat gattctcccc tcctgtttta ttatatacaa caatatgatg
     5161 tatttcaact cataaaatat ataaatactt caaatgtcat tacaaataca acaacttgtt
     5221 gtactctact gagaaggggg gtgtattaat gaaacagaag caatatgcaa aggtcattag
     5281 agaaaagcga aaagcactcg ggtggacaca aaaagaatta gctaaaaaaa tcttctctac
     5341 acaacaggca gtagcaagat gggaaaattc tgtaacagaa cctaacctcg atagcttaac
     5401 agctctttct agagccctag gaacccctgt aagccatttt ctagacaatg ttgacataga
     5461 ttatgaggaa gagtttttgg ctctctatcg ctctcttagt actgaagatg caattcgaac
     5521 aattgactat atgaagctat taaaaaggca agaaaatgaa cgcaatcagc tattgaaaga
     5581 ttgaaaaata aataacccac cgaactttaa cggtgggtgt attgagatga aataagatta
     5641 agagctatgg ataacctgtc tagtttaata taattgggtc tcttctattc cttactctcc
     5701 gtccatcatc tcttgttaca cttgtaaatt gataaatagg aactaattca actcctgata
     5761 atatcaattc ttttattact tcatctatcc ctgccccttt cgctagatat actaaaaaac
     5821 tactgcttgc tttcacattg tatagtttct taatatcctt agaagctcgt tcaagagtga
     5881 atccttgttt aacaaagtag cctctgtttt ctattaaagc atttttcacg ctgttatccc
     5941 tgacatttcg taggcgttca caaacagtgt taataagttc tttttctgtc attagatatg
     6001 tcccttgcca cgataagccc tgtaaaactt catgtgcttc atctaaaatc aattgttttt
     6061 ccttgctaag ttctgatcgg gaattttgag gaaatactct tttagcctct ttttcgttaa
     6121 aacatatctt aacccattca taagaaaaat attgatatgt aaaatgaaat ttttccagaa
     6181 gtggaaggat atcttcattc aatttttgaa tcacagaagt tgcattaaaa acgtctaatc
     6241 gatagacatt gcttctctta tgtttaaagt ttcctaacgc tttctcttcc tttaacctat
     6301 ttaggtattc gagcatcttc aaaggaacat cttcatcatc taattttttt atcaatccta
     6361 taaaagtcat aagtgatagt aacttattac atttatctat gtcataagtt ttaccatagt
     6421 aagaactcat ttttttagct aattgttccc ccgacaaaat acaagtcaca ctttccttac
     6481 tatcatcaaa aaaattaaca tcagaaagca agacatcata gacacgtcgc atttcctcta
     6541 gataattcct tattgtcctg aaaagtttgt ctttcattga ttcaagagtg tcaaaaagta
     6601 cttttgattc ctctttcaat tttcgaaaat gtgtaggata caagtctagg ttctctaata
     6661 gccaattaaa tgtttctacc cgactgtctt tctttaaaat tagcatcaca actccaataa
     6721 aattttcact gatatcacaa ttactggaat gacaataaaa aatggaatgc ccagttttac
     6781 tggtaaaaat actagcagag ggattatgat cgttgtgaaa cagacatctt aagtttttac
     6841 ttgacttaag taatttattc atatcaatgg ttaacaatct ttcataaatc tcattaaaag
     6901 acatgtcgga ggaatcaaac aggcagtctt taaaccactg tttcatttct tctttgtcgt
     6961 cattttttat caacttaaca aaatcggttt cattgactag ttcatactct ctccctgaat
     7021 cactttgaca atttttagaa catgtcatgg taaaatcaat cggaagctca gtaatatcta
     7081 acatgttgtc ttcattaatt attatcactg catctgactt atatcctcca aaaaacattc
     7141 tattcgaagc atttacattg tcatcacacc cggggatgtt ctttttcaag tattcgtaaa
     7201 cagcaaccat tttaccatag tcagtaattg cttcttttaa tagaaagaca atcttgtatt
     7261 tttctagatt ttcagagtac cgaatacttt tttgtatcgc aaacgcagaa ttatgaacta
     7321 aattgataga taaaaccttt tctatgctta agtatctact atcatcttta ctcaaatcta
     7381 ctaccttccc cttctcatct ttaacagtgt tatcgacatc cagaataatt actctggttt
     7441 ccacaaaact ctccttgtca ttgcctttat tctttgtctt gcttggaata aatgattgtg
     7501 atgaatttag tatctctaca aactcatctc ttgtaacttc tctatagtta tctaaacacc
     7561 tatctcttac atcagttttt tctttttttg taactggctt ctctaataat ttttctttat
     7621 ctacataaat tggaatatta atcattgatg tttccatttg tgtttcctcc ttataaaatt
     7681 ctgttaaaga ctaaaacata cagataataa aaacaaactc tttattacta tatgttttag
     7741 tcttatttgt ttactcaacc tttataccgt tccatgaaac tatcaatatc agatttttta
     7801 aatctaatga ccgtatcaat ttttataaca ggtaacccgt attgagaaac atattttcta
     7861 atcgttccta ctgatacacc tagatattca cttgcctgct gatagtccat atactctaga
     7921 gtgactccag aaataatgtc ttctagcata ggctttagca ctgtaattat ctctttcact
     7981 tgttgttcaa cttgtataga aacaatgcta tcaatgattt gtagagacat atttccccct
     8041 cctttcgtga ttgcttgaac aaattttcta agaattatct tcgtattttt tgctcaaaat
     8101 aaaagtattt tgttattaac cgattaatca aacaaaaaat gattaccagt tcatcgacat
     8161 acacataata cctcgataaa aaaggggtgt caatagtttt gtaaaattta tttacaacac
     8221 cccttttttg aggtatacta attttaggag gtgtggtcaa tatgataaaa cgtccaccaa
     8281 taaattattt agaacgaaaa aagatactcg gaacaaaaat aaaagctata agaaagagta
     8341 aaaaattaac acaaccagca tttggtctta tgattaacaa cggtcaatta attgacaaaa
     8401 agactatcta cgaatgggag aagggaacct atttaccaat accagagcga ttatcccgaa
     8461 ttgctgattt gggaaatatg agtatagagg agttagtttg tggcaatgta gaagaatata
     8521 ttttgggaat tattctttat agagatagca ttgtactaga tggtataaca tttccagata
     8581 aaaacctgtt ccagcatctt cgtcaacagt tcccaccagt acactctaat ttagatacat
     8641 ggctggatag atattccaaa ttagaaccag agatgcaaga atttattgct aacaagactt
     8701 gtaataaagt taaaaatgaa aaaattagtc ttttcaatat cttaaaaata gaagaattat
     8761 tcatcaatgc tattgtagaa gaatttgaca ataacattct tttcctcact agcagtatag
     8821 aagaactact agaaagaatg gtagatgaat ggctacctat tcaattgaaa gacatgtctt
     8881 atcccgaaga agctgtgaga gaaattactg ataatataaa caagctagaa caaacgattt
     8941 catcaattgg gaaaaaatac actaagaaaa aaatgaaggg aggtgatacc atctagtggt
     9001 aagtactcct tgtaaactaa gaggagatga tttcatggca aaaatatcaa agtacaaaaa
     9061 gaaaaacggt caagtggcgt ggatgtataa aggacacatt gccaccgatc cacgaactgg
     9121 tgaaaaaata aatactacga gaagaggttt tagtagcaaa caagaagctc aaaaagatta
     9181 tgacgagtac agacatcaaa tattatacgg tgttaaaaag aaagcacctg atatgacatt
     9241 tgaagacctg tataatgaat ggataactca tcagcgtacc agtgttaaag cttcaacaat
     9301 cgcaatctca gtaagatacg caaataatca aatacttcca gcatttggta agcttaaact
     9361 atcgaatatc tctgtcccat attgtcaaaa agtggtagat gaatggcaca gtaaatatga
     9421 aagttacgat tatatgagaa aacagactgc acaaatttta cgctacgggg ttgcaatgca
     9481 atatattgac aataatccta tggaaaaaac acttcttcca cgaaaaaaag agtatgagaa
     9541 aaatcgtaaa ttttactcaa aagaggagct taataattta ctggatgctt ttaaagattt
     9601 tggtaacatg aaacaatacg ccttcttcag attattggca tacacaggta tgagaaagag
     9661 tgaagtactc gctttacagt ggaaagacat agacacgttt aataaagaac tacatgtcaa
     9721 taaaactctt gctgtagatg aatttggtaa agttataatt caatctccga aaacaagagc
     9781 ttctagacga gttatctctt tagatactga gactctttct atcctcaaca actggaaatt
     9841 acagcaaaaa gaagagtatt taaaacttgg atacaatact agctctaaag agcaacatgt
     9901 ttttacaaca gttaaaaaca ctctatacat tccaaacact gttaatgatt ggttaagata
     9961 tattttgaaa aaatataact tgcctcgtat tacacctcat gggtttagac atacacatgc
    10021 tagtttatta ttagaagcag gagaatctgt caaggtagtt caacaacgat tagggcatga
    10081 aaatagtaaa gtgacattag acatctatgc acatattaca aataatgcac ctaagaaaac
    10141 aggtcaagac tttgctgata tgatggctca tcagtaaaag agctggtcaa aatctggtca
    10201 tttctagtat acaggcaaaa caaaaaagct agaaccctta tatatcaagg gttctagctc
    10261 ctataacagg tttacccaac actgccttcc aatgactctt ttgggaatca gaggaaagaa
    10321 tcaggcttta tagagatatt tcatctaaca aattacttta gactgacagt atatatcagc
    10381 aggtactgtc atcgggaacg tagccaatat cgtatccaaa acagcaggta gagaagctat
    10441 ttgaggattc aagttattct atttcaacca tgaagttaag ttcattaaaa ataaagatag
    10501 ttggtatttc aataattttt gtatagagtt aatcacttta ttgatgctaa ttcatcttga
    10561 ttggtattat aatcgtattc aagttgcgtg gcgtgctttt gctagaaaat atataactgt
    10621 tctttataaa gaaatataag gattaaacat tttttatcat agagttttac ttatctatag
    10681 aattatgtct gttcctaatt cagtttgatg aattaactga taaaaaaata tctactattt
    10741 aaagatacag ggataagtaa gtaaaaaaat aacatgtcat tagatagtag acagttccaa
    10801 ctctttattt accttttttg tatgctgcac tgtcaatcca agttcctaaa ttgttgtatc
    10861 catcatcact tatataatca tattggccat caataatttc gtcaaaccac aaatattctc
    10921 catctggttt ttgcccaatt attttaatta ctgattgact agtagttgtc atctggttaa
    10981 aatcttttat tttacctata ttaacggcca aaatagcatt tcctcgctta taactctcct
    11041 ggatttcata tttaacatat tctcgatcaa gagtttcttt acctatcagc accacagtca
    11101 ctgatgttcc aattaattga ttatctatcc agttttttat agcaatatct ccattacgtt
    11161 taactttctc aaattcagcc ttatctacaa aaccagcttc ctttgttcct cgcaaagtcc
    11221 agctgtttct aactaccatt gctcttgtta catctgcata atggaaacta aaaaaaaccc
    11281 ttctcaccat attaatcctc ctgaatacta tttattaaac tgacaatttg ttcaactttt
    11341 ttatctactt ctccttcagt atctaatttt tctagcgctt gaaatttact ttttaaggaa
    11401 tcatcaaaat aatcactgaa attctcttga atcttatcaa acaaaacctt acttgcacct
    11461 ttggtaaatc caatcggaac tactatattt tcattttgaa cagataattc aaattcttca
    11521 atcattccat ctgctgtaac tatttcacct gattcaagtt tgtttccaaa aacaaaaata
    11581 ctaatcccag actctgcaat aatgttttct cgatatcttc tccactcatg aggcatatca
    11641 aggggaagtg gtcttaaaga aagtacgtct tgtatgttat tttttcttaa atttcttctc
    11701 tgctttagaa ctcctgaaac aatactatta ccaattccaa gtccgtaacc tgatactatt
    11761 ctataaccgg attcagaaag cctatatgct aatttctcga caaattttaa tcctttcgat
    11821 tcagatgtaa atggagtata atcaacagca cttcccgaaa taaaaatagt ttttctcaaa
    11881 tacttttttc taatactttt caaaatatca gtaatctcag aataatcatc tacttcaaca
    11941 acgtttatcg aaaatctgga caaatcattg atttgtagtc ttctttttgt cagttcataa
    12001 ttatatctct cttgatcaaa aaaatcctca gaatcgacaa aattttcctt taatattttt
    12061 ctcattatac aataatgagg tctttggttt tctctcaaaa cccatcttat atctgataaa
    12121 attctttcta aattagggtc tgtaaaacta aatcctaaaa agagaaaagt ttttgttaac
    12181 aaatctcctt ttaaagtttc tttaaataac gcgtacgaat catcatcata tttttcataa
    12241 tcatctcttg ttattactgt cgaactgaca tcatctatat caccatgcat tttataaaca
    12301 attgtatcca catttggatt agttgttgct aactgagtat aatttttctt aacctgaggg
    12361 tttttataag cttctcggat tgatttttct attagatgat cataattagt tgtccaataa
    12421 gtttcaatag gtaaacttgc tagtattcga tgattttcag atatttctaa ttcttcacca
    12481 aaattatcca taagtatctg attcaaaatt cctcttgatc caccattttc atttttataa
    12541 tattgagcca gagtaactaa gtctgtttct tttttagaat ccaacccgat ttctgttgca
    12601 ggatcattaa gtagttccgg ccaacttaca tatccttgtg acatcgataa tcctgcacct
    12661 gcaaatattg ctgcatttcc tttttcaata gcttcagaga acctctttat taaaactttc
    12721 ttatccattc tacccggttc ctttcgattc agtaaattga ttagagatta gtgaaaatgg
    12781 tgtttttata taaaatatct aatgtattac ttcctttagt gcattccaca gcatctaaag
    12841 cccatttttt caaatcagct tttcgtaaat aagttaattc acttaaattt aaagaatatt
    12901 ttatttgcag attttcttgc gaagaatagt gctttataat gcttctaaca tcctcggtgc
    12961 tcatttgagg attactttcc aatcgtggat tctttttttc cactattgaa gctgtcgtca
    13021 gttcgctata tagccatggc gaatacgtaa atttctgttc attagtgatt tcattgctca
    13081 aactaattga attaggtgta ttaagaaaaa acagacattc tgtactatca atcatattat
    13141 ttaaagctgt agaaagcatc atatgaacat gactagttgt tacatttctt ttatcataat
    13201 catatgtttt tgtttgttcc ttatagcaat atttttggtc tatttttttt aataaatcag
    13261 aactgtatcc ccaaaccgaa gaatcaataa atgcagtaag accaaaattt tcaaatagcc
    13321 atgatgcaaa acgtatagct aatttttcat cagcatgaga gtgagatata aaaatatctg
    13381 cttttatagt tggaaaccaa tctttaataa tttcgtctgc atctagaatt ttgctgtcct
    13441 tagaataata atcaactaaa tcattttcac tttcaaatag attttctatt ctcttgttaa
    13501 tagatttttg gttgtgatct atttgattta ctacatgtgt atatgattgc gagcttgagg
    13561 agaatgcatt taagtcagca tttgtaaaat ccaattcata tgccttatac aaggatatcc
    13621 ctccattctt gtattttgat ccacaattta ttaggcaaca taacagtata ccctatcgtt
    13681 acatatccca ttcattatta taccagtatt tcttcgtgac acataagggt gtaacataat
    13741 aatttcaaac gaaatgacta caagtagtca ttgaagcaaa tcttcataag acacctgtag
    13801 aatatctttg atggctttca attgatccaa cttaatgtgt tggcggccac cttcaatctt
    13861 aaccaacgtt tccctagtta tagcaatttt acgaagctgt aggtctctaa tcagctcggt
    13921 ttgaccaatt cctttttcta atcggactcg tctaatattg ctaccgatta cattttcttt
    13981 atcaataatt ttttcctctt tcaagaatag cacacctttt ggactaaaat tagtcctttt
    14041 tcttattaat cttaaagatt cggtatgcta caatgggact agtattagtc catattcaat
    14101 tttaaggagg aatttttttg aagaaatttg caatcattct aacttgcgct ttactcatcg
    14161 gaactataag cgggtgtacc acttctgaca caagttctac aagtacggaa actagtacaa
    14221 caacctcaga aaagaattac ccaatacatt tttttccggt tccagatgga tttgaagctt
    14281 attatcatga taaaaaacta gatgttcctt tatcagaaac tgaatctact gaaaagattt
    14341 tgccaattga aattgaaaga aatcaagacg atataaaact aaaagctgat cttcccttac
    14401 tgaacagcac acaaattgat agacatgaaa atattaaagt taatattgaa aaaggagcgt
    14461 tagcacaact ttatactgaa aaagttgaac aattttttga ggctggtgcc aataaagact
    14521 tttcaaaaat aagcgattat tcggataact tttttaatga gacccaagat gaaatcacga
    14581 actcaacttt tatggctgaa gatatgtaca atatgaagtt gaaaaaaata aagtcgtatg
    14641 attatccatt aaatctaaca ttaaataaag ggatactctc ttttgtatta gcaggttacg
    14701 ttgactatga atatacagca gattatttgg agacgcccct tcaacacgat aataacgata
    14761 ttaattttag atttatctac aattcaaaaa gtaaaacatg gttagttgat caattgagca
    14821 ctataaatac gggatttcat gaattaccta agcctgacaa tgaaactgct ttggtcatta
    14881 aaacattcta aaaaagaaac gttgaaggaa taattattct ttcaacgttt cttttattgt
    14941 ttttcagttt acggtcttac ctgagcgaat aatctgattt ctaaatgatt ggaaaacttc
    15001 ttgtggttga taataatctg tatctatttc aaatgtttct gagaataatt cattgaattt
    15061 ttctactgaa atgatgattt taccattctc ctctcgactg ttttctatta tttctgcctc
    15121 taattcaaat aaatctagtg acgaactaaa atcataaatc ctgtcatatt gaacaaaaaa
    15181 atcaagaaaa agttgctcaa actcttcctt atttatcact agtgtttcaa gcggaacaac
    15241 tttattttca tcagccattc ttccaatttc ctcactttca ttttcaattg tccattgttg
    15301 cggacgtatc agattttctc tatcattttt cagtgtatag atcacttgta atacacaaca
    15361 atgctttgta aacttatttt tttagttagt cgaatctttt taaatgtgcg tttcatacca
    15421 tgcgcttgat atattttctc ctatttttta aatcgtttat cacaactttt atcgtttttt
    15481 cttctgaaaa aaatcttgtt tacatcgtta catagtttcc aaaaagatac attactagta
    15541 gaaacaacaa gattaatttt acttcccact gaaatgggaa gtatatttta aatatttttt
    15601 ctccttcaac ttacactata aagtatctta atcacacgat atgctagcca ctaaattcat
    15661 catatttgat tcctttttta agataatgta gcatcttttt atccaataac gcaattgttt
    15721 ttaaggataa atttcttaag atggtacatt tcaaaatggc atttgacata tgaaacaaaa
    15781 aagaaagaaa agagcataag ggaaaactga tagcaccttc ctacaggaag gtggctaagt
    15841 tggctgtgcc aactggtttt ctttcaaaat catttcataa tttttgatat cataaaaaaa
    15901 tccattttgg acctattttc ggtcataatt tagtacctac ttttggtcat agtttcgtcc
    15961 gtagttttgg tcatagtgac taaggctttt ctgttcacaa aattggttta aatttgcttt
    16021 ctttaacact tcatttatat catagcaatt ataatgttta cacaacatca ttcctaattt
    16081 taacggttgt tcctctttat attttttaag ttctacattt ttttgcgttt aattactttt
    16141 ctcttttctt tatattccgc tccaactttc tctttaatag tgataataga attacatatt
    16201 tttgttaaac ttccttaaaa aaattggatc tcgattactc aaatttgctt gcaggctttt
    16261 ctaattgctt tgttttttct tcctaattat ttgattttat ttccattttc ttctagtgaa
    16321 aatttattat ttttaataat cgtacgcgta gaactgattt cattttttgt atctgtttga
    16381 ttatctaacg tttgaaattc aaagttttga ttggctttat caatatcttt ttatactact
    16441 ttatttgcaa gatatatttt cttgcttaat ttggtcatag tttcattcgt cacttcggtg
    16501 atattttttt aatttgcaaa taaaaagagt taaaccgata tacattaggt tcaactcttt
    16561 tgcaggtttc atgggtgtac tatcactttt aatctgatag atgtaaacct ctctttatct
    16621 ttttgttact tcaattttca acaatagctc ctttataggt tgtcaatatt gctctacgta
    16681 ttctggcttt ttaacccatt atttctctaa agtggaagag aataatcgct taatccccac
    16741 taattccttg tcgtaaattc atgtcaaata caatttttaa aagccattta attcatggat
    16801 tatctcttca cttttttcta gataacaata tttcaagttg ttttttatct catgtttcat
    16861 ctttaattct tcttgaatca tcagttcaat ataattagtg accgtgattt tttcatttgt
    16921 tgcgttcttt actgattcac ataattcgtc aagttctaag aaatcatgcg tactaatctt
    16981 tagaattaat ttcatgacaa ttcccccttt tattttttta atagattatc gaaaagcgga
    17041 ttaggatcat taagtgtatc tctgaataac tcgttgtctt cataggtaaa tcttcgtctt
    17101 ggttttgatc ccttatcatt ttcacggatt tcactatcaa taatcactgt ttcatgattt
    17161 gaatcaaatt ttttcgctga ctttctgtct tcaacgatat tgattttttg tgttttttct
    17221 ttcatttctc cactatcttt ttgaacgttc tcccgaacat ctactgtttt gaatccttga
    17281 ctacttgtag aactttgtac attctctctt acatccattg atttaaaccc ttgactactt
    17341 gtagaatttt gtacattctc tcttacatcc attgatttaa acccttgact acttgtagaa
    17401 ttttgtacat tttctcgaac atcaattgtc ttagtacctt gaccatttga agagctttgt
    17461 aaattctctc ttacatcaac attccgtccg acatgaccat tcaatgaacc ttgaatattt
    17521 tctcggacat ctactgatcg gccaccttga ctacttgtag aattttgtag attttctttg
    17581 accaattttt ctaatccggt atttgagttt ttagcttctt ggattctttc tttggtcaac
    17641 gcttcttttt cgtccgtatt agctcctgta cgcccttcta cacgtttcct taacatttcc
    17701 tcacgattca ttgcttcagg cgtgtattta gcttcttgtc gcttctgatt aattaattct
    17761 tcagcagact tagcttgacc gtgcatagca tttttacgtt cttcaatatc ttgttgaact
    17821 ttttgctgaa ttgcacttac aggattatta ttcgattcag cattattttt cattgcttca
    17881 cgcatacgtt cagaactaga cataattcta tcggcatttt caaccttagc accatctttt
    17941 ctatccgaaa gacgtgttgg catggaatca gcatattttc cagtttcatc ttctttattt
    18001 ttagctaaca taggtttgct atttttatta atagcggaca ctgtaccatc atctaaacct
    18061 tctttgaact tattcttggt atcattagcc atatcacgca tactcttaaa tggttttatt
    18121 gctctttcac tagccattgt agctccatct gaagctaaac ctgataatcc acgttcatgg
    18181 gcatatccca aagctcttcc agttttgcca gctttttttg atagagtttc gccgaaattc
    18241 ttttctggtt ttcgatcttc tccttctcca ctgtctgatc catttccatt tccgcttcct
    18301 ttagcacgat taaatccagt tgatcctttt ctaaacagca ttgcgcccat accaaaagca
    18361 gaagcaagtt gcccatagcc ttctttgagt ccaatatcaa caccaaagta tctcatgact
    18421 gttccactgc cttttatcag cacaaacaca gcacagatat aagcaattgt ttttataaaa
    18481 atatttgtgg agataccttg tccagcattt aaaaaattga taaacatagc aaacatagat
    18541 agtccaaacc cttgaaatcc aactgttaag taacattgta aaatatcgga taaaacaact
    18601 tttgaacgtt gtcctgtttc aatatctgtc gcaaaaacaa gaacacctaa cacacgttta
    18661 aatgcaagtt ctaatattgc tgtaatgatc acaaacgcgc tgaacacata ggcgattgcc
    18721 aacgctgtca acccaatcga aatcccccaa agatttgctt gatatcgata ataacctgat
    18781 ttcaagctat cagaaaatgt tgataagaag ttatcagaaa actttgtggc aacaaattca
    18841 ttattagaat tttcaactaa ttcatagcga agattatccg cagctggatt ttctttttcc
    18901 aatttatcaa tcactttatc cgtcaacact tgtgttaaat ctgtctgttg aaaatttttt
    18961 cgtgttaact tatttcgatc ctccgtttta tcaatgcttg agtattgatc tgttttatta
    19021 atatatgcca gatctgttac tccttgtttt atcaatgacc aagcgactcc gtcatctgaa
    19081 tttgtaatcg tagtagcatc attgtaaaat attttggcaa attgaatacc gctagaaatc
    19141 attgttggca ttaaaagaat taacgctaca gacatcacta tattcattcc aacagatctt
    19201 aaatctattg ttccttttcc agtaatcatc ttgtagccaa caaacatcaa tgaaaggatc
    19261 attaatccaa cgacaattgt atttatgact gattgataaa cattatttag ccctgtactt
    19321 tcaatgaaat tgaacaaatc caacacatct ggaatcatat tttgaatcga atcagtcaca
    19381 gtaaacatgc cttttaccac agcccatcca agccaaatcc cgaactgact aaggaaatct
    19441 ccattgtatt tttttaggta aggtctataa agagaattat tcaaaaactc tgtttgactt
    19501 tttgtgtcat caaaccaatt gaaaatgcta gcttcaacaa tattacttgt aaaaacaacg
    19561 ctaatcatta aaacaagtac actgagtgct aaccagttta tttttctact aacgattttt
    19621 tctttaattg ctttcatagt tttctccttc agcccatttt cttaggctct cagagagctg
    19681 ctgagccatt ttttgattaa ttatgatatg agttacacaa ttgtcatttt cagctcttaa
    19741 atcgagaact actgtgttga agcagatact ctcgattaat tcgagagtat ctgtattttg
    19801 atcaagacac cacatttgat ctattaagcg atttttaatc atgattcttc tcctttcaac
    19861 caattatgca tagtaatttc aggtaactct ttgatttcga aagctttttt gaactgatct
    19921 ataggctcta cattttctaa aaattgactt ttatatcttt tctgaagtcc attcaaaaaa
    19981 tatgttacat aatcgtataa cccttcttca gtttttcttt gaaaaaagca tgcttcatgt
    20041 tgttttataa tacactcagc aataagatac tgagtatttt cagataaata ttcaatgatt
    20101 tttctgatat cttctgtatc tacattagcg tttctcaaat agtgatacac ttgaaataat
    20161 tttcgaccat cttcgttatt tgcgtcataa agaacactaa tcttactgta aatcctcttt
    20221 atattaatat cctcttcttc cataacgaga gtagtctttg atatgttctg tgaagtagtc
    20281 tttgtatacg tatatacaaa tttgtacata cctctacctt tttttaaaga gacatatctc
    20341 tttttttcga tacccctctg acgaattttc ggcatatact cctttttttc gcaatccaaa
    20401 gtaggaaaag taatttctat caatttatgt acatttaaaa caatgtacat aacatttgat
    20461 aatgtaactg ctcttttctc ataataaata tttctaaaaa cccttttgat aatccccttt
    20521 ttctctaagt acacaattgc atcttttata gctcgttctg ataatcctgt ttttttgctt
    20581 aattgcttat atgatttttg caaaatatct tcaagaaact ttttttgata accaagtagc
    20641 tgaccatcat cgttaaaaat ttttttaggt ctataccaat aggtaatttc tgctaagata
    20701 actattgcat tcaaataggg cttctcaaca ccttttttcg ttttttttct taattgatcg
    20761 taccatgtgt gtggattaca tttccgtcaa aatttatttc tctaagtttg tcaactacaa
    20821 catttccaga agaaaacacc ttttcacctt cttttttgat ataatagcta taaataaagt
    20881 tttctcagca ctttattatt gagtgttccc ccagtctgca aacttttggg aacacttttt
    20941 ttcttttttt aaagcatttt tttataattt ctacttcatc atcttccaat attttcccct
    21001 cttttcattc gagcaaacat aaactcatta actgcctttt tcgaataaaa ttttcttgcc
    21061 cctaattcaa ctacgggcaa tccctcattt ctcacagcat tttttaaaaa gttttcactc
    21121 acatttaagt attccgccat ttctttttga ttgagatatt cccttgtcat ccccgcattt
    21181 tcttgagcct tttcaatagc ttttgtagca atttgataaa actgctttag aaacatttcc
    21241 tcctgttctt ctgaaatcat taattttatt gcttccacaa aaacctctcc tttttaatta
    21301 attattctat aactcctcta cctttataag tacaaataaa tacaaattgc gtaaaaaaat
    21361 gtatttattt gtatttataa gaataaagta tcatactcca gataaaaagt aaatactttt
    21421 aaatactttt attttataaa tatgatatac tatctttaaa aataaaaagg agaaaaaaga
    21481 tgaagccaaa tccttcagaa gcaggaaaac gaatcaaaca attacgcctt tcatgtggtt
    21541 ttactatgga agaacttgga aggaaaatag ataattcacc acgtgcgacc atatccaact
    21601 gggaacgtgg gaccaattta ccaaatccac aaaagctgaa attattatca actatcacta
    21661 attcaacaat tgactggatt aaatggggaa ctttagaaga atatataact tcatacctta
    21721 tcgatatagg gtatgagcta tatattaagg acttcccaga aatacctcac aaagtcttta
    21781 aagacattca agaaagatat agtgatacgt tttcgctaga taaagactat gagttattga
    21841 atcctataat aaaaaatata tttacaaaat attactcaaa agattttgaa atttatctaa
    21901 atgagatact tgctaataat atcaatcctc tgattgaaga gtacgctaac caaagacact
    21961 ctatctctaa agagctattt ttcaaaagag ccaacagtaa acttcaagac aagataagaa
    22021 atggtgattt caaatacggt gaaaaagaaa gaatgattga ttctttaaaa cagctattag
    22081 acgaaatgga cttagcctat aaagatattt caaagtattc atctgtcgag gatttctttt
    22141 ataaaattac taaaaaccaa tttgaaacag aaaaatttat ttcagatctt agtaaaaaat
    22201 acaatttccc atatgagaaa aatagtataa tcgcaaaatt tttaactgat aatcacgata
    22261 aatttaaaaa taattgattc ttcaaataac ttctctacct gcaataatga agtaaggaga
    22321 gaatcaaatg gcaacatttt ttagttataa attacaaaat ggcgaaaaat attggggatt
    22381 tgaaacttat ttagggaaag atcccaagac tggaaaagat atacgtacaa agcgtagaag
    22441 attcaaatca aaagctgagg cgcaaacagc acttaataga ctacttgtag actttaaaga
    22501 aaaacaagag tttaaaatca atgttacaac ttttcaagag ttgtatgaat tatggattga
    22561 taactacaga acaagagtaa aaccgtcttc tgttgccgtt gctcaacgtt tttgcaaaaa
    22621 ccatatatta cctgcttttg gggaattacg attggataaa ataagtgtta gctattgcca
    22681 aaaacaggtg aatgaatggc acaaaacatt aaaacaatat ggtttcttga gaaaaacaac
    22741 tgctcaaata tttaaatttg gagtagcaat ggaagtctgt aaggataatc ctatgtctaa
    22801 aacattgctt ccaagaaaaa ttgaagaaga acaacccttg aagttttaca cgaaagatca
    22861 attacaactg tttttacaga atactaaaga aaataattca gttaaactgt acactttttt
    22921 ccgtttatta gcttatacag gaatgcgtaa aagcgaagca ttggcgcttc aatgggaaga
    22981 tatagactat ttcaataaaa caataacaat tggtaaaact atcgctcaag acgagtttaa
    23041 tcaagtagtt ctacaagtac ctaaaactaa aaattcatca agaacgattc agcttgatga
    23101 ttttacactt aaacaactgc gtatttggca acaagaacaa atgaaaataa tgatcttata
    23161 tggctataac acgaattcac caaaacagtt tcttttcact acaaacacaa acaagttgta
    23221 ctacctccaa gtagtaaacg attggttaga ctggatttac aaaaagacac cgatggagcc
    23281 tcaaattact ccacatggat ttaggcatac ccactgtagc ttattgtttg aaagtggtgc
    23341 gtcgataaaa gaagtacagg aacgattagg tcataaagat attaaaacta ccatgaatat
    23401 ctatgctcat gtcacaccac aatctgttaa gaaaactggt gaccgatttg caaaatttat
    23461 gggcgaataa ttccaaacgt agccaaaaac gtagccaacc attaaaatac aaataaaaaa
    23521 acgctcgaaa cgctttaata
//
LOCUS       DASHHB010000039        23156 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.040, whole genome shotgun sequence.
ACCESSION   DASHHB010000039 DASHHB010000000
VERSION     DASHHB010000039.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 23156)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 23156)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..23156
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.040"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(21..221)
                     /locus_tag="U2K71_001792"
     CDS             complement(21..221)
                     /locus_tag="U2K71_001792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM7315872.1"
                     /translation="METGTVKWFNSEKGFGFITAENGNDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEEGQRGPQATNVTKA"
     gene            complement(674..994)
                     /locus_tag="U2K71_001793"
     CDS             complement(674..994)
                     /locus_tag="U2K71_001793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315873.1"
                     /translation="MKKRLLWCLLSLSLSLTSQPVFAEETQRFPITGTRLYKNVYVQT
                     PDLDSAIPETEERSLQPRFGYFWYCPTCDYESCWHFLHSTAAAFAFTHTQKYDGHHPV
                     IYEV"
     gene            complement(1497..2615)
                     /locus_tag="U2K71_001794"
     CDS             complement(1497..2615)
                     /locus_tag="U2K71_001794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315874.1"
                     /translation="MAKKDKELLSQLTSEGAKGPFVTIMLNTHVAHQDVEKDQIKFKN
                     FAKEAKKRFEKKYSEHDWSPFQEKIENLLGDPSFWRSATTSVAVIITADQTYIHRLSI
                     PVDDQYYVSDLPYLLAIIKNGQFNYSYYLLGLNRDSMKLYEVSNKQVKEVSLPEGAPV
                     DVVTALGSELTGGGLNFSSNGGGGARDGVTFHGVNPKDEEVEIDWVNYYQAVDTYLKD
                     SFENPEQWPIYLFALPENQTMFKKIAKNPYYCADVAISASPAQLSFRDIEEGAKKLSS
                     ELAAQESKEYNKLLDKKFIDQLVDIVPAAKEGKVSHLFIATSNLADGFGEDPETEYDR
                     RQVLNALADDVIINGGKVFILDQKDAPDEKSLTAILRY"
     gene            3086..4393
                     /locus_tag="U2K71_001795"
     CDS             3086..4393
                     /locus_tag="U2K71_001795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase"
                     /protein_id="HEM7315875.1"
                     /translation="MSIKKTRLVLLLASLILCLASFLPAVAVQAEDTFKVNAKAAFAV
                     DAQSGKILYDQDGEKTMGIASITKIIGLYIVLDQVKEGKLSWDDKVSISDYAENLSVT
                     PDLSNVPLHKENTYTVKELFDSAIIQSANASMVALAEKISGSEAKFTERMKEQLKDWG
                     IKDATIVNASGLNNSYLGENRPEGTGENDENQMSAQDVAIVARHLILDFPEILDVSST
                     TTQMFGENTQSPVEMVNWNWMLPGFVNYKEGVDGLKTGTTELAGACFVGTITKNDQRI
                     ITVVLNADGHAENPSARFNETARLMDYCYDNWSEKELGKANASIPSLKTIDVKDGKES
                     TVPVVLKSPVKVWVRNDMDTGKLTITPTLDKKQVTDNEIQAPMDKGTKIGTATITLAE
                     DKLGYLEDNNAPSTEIITDKTVEKANIFVIGWRRVAEFFGNLF"
     gene            4514..5713
                     /locus_tag="U2K71_001796"
     CDS             4514..5713
                     /locus_tag="U2K71_001796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEM7315876.1"
                     /translation="MNLTRDLIFLVLYLIGVFSIVYWRKQKKDPSLFFYSSTWTFAVI
                     LTYFVVLEIFYSTFYFLIPLVFFSIFAFSYFSEKRRLLNGLLFNIFLISFGIYLFVLL
                     YETQNIFLGGLIALITIPLLLVFLFGIYGLIVFLFWNGVTVLRRESHSLANLLTLILA
                     IFLTLFLVFDFFLLKYLPQWINALFFCIPLILIYLFIVFYNFLTVSFLYQFNRPRYNQ
                     DFIVVLGAGLINGETVSPLLAKRINKAIAFYWAQSRATLNPPILLMSGGQGADEKVPE
                     AMAMKQYAMEQGIPERDILVETNSTTTLENMRYSKEIMDQQMKGPYRAIFSSNNYHIF
                     RAGLYARQAKLKANGIGAKTAFYYLPNAFLREYIAILMMNKKRHMIICGLLFAGSAFL
                     SLITLLF"
     gene            complement(5740..7008)
                     /locus_tag="U2K71_001797"
     CDS             complement(5740..7008)
                     /locus_tag="U2K71_001797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="voltage-gated chloride channel family protein"
                     /protein_id="HEM7315877.1"
                     /translation="MEKQLPSPVRILMYMLKWLVVTAILGVFMGSLSAFFLNSLTFVT
                     DIRLAHPWLLYLLPVSGALFAYLYAYHGGLSSRGNNLVIDQGNGGEEKIPLRLIPLTL
                     FGTITTHLFGGSVGREGTAVQMGGALADNIAHLFRLDKAEREILVISGISAGFSSVFG
                     TPLAGTLFGLEVLAIGKVRTEAIFPSFFAALFANFMTESYGVSHLHYQMGVIPEWSML
                     LFFKVFVAGISFGLIGWVFSRSIVFLKARYAQWIPHPVWRNTIGGAVVVAAALILQTQ
                     RYLGLSLPLLQESFNGQAHAYDFIGKLFFTVLSLGAGYQGGEVTPLFEIGATLGAVLA
                     PLLHVSVPFLAGLGFIGVFSGATNTPIACFVMGIELFGSQAAAYFFMICLISFMCSGN
                     SGIYSAQQVAVKKGILFLPIVKRIKEKNKV"
     gene            complement(7178..7819)
                     /locus_tag="U2K71_001798"
     CDS             complement(7178..7819)
                     /locus_tag="U2K71_001798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010728800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G-binding protein"
                     /protein_id="HEM7315878.1"
                     /translation="MEQTMKPYQYFFIKREVEQLLNAYSSVNDSKTVQTVQALAAEKI
                     RDVLGYEPSEIDTFLTTVLDVKLTKTQAERALEELKQIVQPFMQPSKPQIEKLFRKVK
                     KLKQPAWNEIDLQAHTYIGWNDPGTQKKFMVYYEDGKLKGISGTLSPTINKGICAICQ
                     KTSNVSLFLSTTKAGSDGTYTKKGNYICHDSDQCNQQLTKLAPFYEFAERVKK"
     gene            complement(8228..8731)
                     /locus_tag="U2K71_001799"
     CDS             complement(8228..8731)
                     /locus_tag="U2K71_001799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM7315879.1"
                     /translation="MTEKWREDEEYMSYVEDLLETEAVKKLANYTQHVHSTRLEHSIS
                     VSYYSYLLAKKWGGNAKATARAGLLHDLFYYDWRTTKFDEGTHAYIHPRIAVKNAEKI
                     TDLSDLERDIILKHMWGATIAPPKYKEGYIVTFVDKYCAVKEAAQPMSASMRKRWQRY
                     FGKESSI"
     gene            complement(8854..9618)
                     /locus_tag="U2K71_001800"
     CDS             complement(8854..9618)
                     /locus_tag="U2K71_001800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HEM7315880.1"
                     /translation="MKEIQSAKNPRIKELKKLHKKKYREEKEEYLLEGFHLIEEAASS
                     DAEIKELFVTQRGMKEWGDWLAANKEISTFLVSDEVMDAVSDLPTPQGILAVMALTAE
                     GTPDFSGGWLLLDNVQDPGNVGTMIRTADAFDMAGVLLGKGTADIYSTKVLRSMQGSN
                     YHLPVLQKDLTEVIPLMKDKGFTVYGTELNEEAHALDKVEKSDNFAIIMGNEGQGVSQ
                     EILSLTDQNVYIPMKGNAESLNVGVAAGIVLYHFSL"
     gene            9726..10001
                     /locus_tag="U2K71_001801"
     CDS             9726..10001
                     /locus_tag="U2K71_001801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HEM7315881.1"
                     /translation="MRKVKMNVQGRVQGVGFRYMTKMVADQLGVTGTVKNEDDGSVTI
                     EAIGNDDIIQKFIEEVKKSPSPSGRVQYVDVQEDPMIEERKKFDVVG"
     gene            10561..11511
                     /gene="yidC"
                     /locus_tag="U2K71_001802"
     CDS             10561..11511
                     /gene="yidC"
                     /locus_tag="U2K71_001802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEM7315882.1"
                     /translation="MNKWKNWLLGSGLVSILLFLSGCVRMDSNGNPDTSGIVYRVLVH
                     PMGQAITYLVENFNWSYGWAVIAMTVIVRIIILPLGISQSKKTMIQSEKMQALKPQVE
                     AAQQKLKAATTREEQMAAQAEMQQVYRENGLSMTGGIGCLPLLIQMPIFSALYFTARY
                     TEGIRESSFYGIDLGSPSMVLVVIAGVAYLLQGYISTIGIPEEQKKTMRTMLIVSPAM
                     IVFMSISAPAGVTLYWVVGGVFSCLQTFITNVMMKPRLKAQVAEELKQNPPKQVVTPR
                     KDVTESAKPTNNKQATKQIKQPKNSTNNKGRNAGKQQKRK"
     gene            complement(11557..11958)
                     /locus_tag="U2K71_001803"
     CDS             complement(11557..11958)
                     /locus_tag="U2K71_001803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEM7315883.1"
                     /translation="MSRQLVLISHGKFCEELKKSTEMIMGPQEDIYTLPLLPEDGTET
                     YKQKFLTVTDSLNDFVVLCDLLGGTPANIISKLIMEGQQITLYAGMNLPMVIEFINSQ
                     MIGSEPEFVRAGCESIVSVNELINGIAEEEE"
     gene            complement(11972..12781)
                     /locus_tag="U2K71_001804"
     CDS             complement(11972..12781)
                     /locus_tag="U2K71_001804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM7315884.1"
                     /translation="MTKSNYKLTKEDFKQINRRSLFTFQLGWNYERMQGSGYLYTILP
                     QLRKIYGDNTPELKEMMKTHTQFFNTSNFFNTIITGIDLAMEEKEGIASKETVSGLKA
                     GLMGSFAAIGDSVFAALIPTIFGALAANMATDGNPIGVFIWIFAQIVVMVFRWKQLEF
                     AYKEGISLVTTMQSRLTALTDAATLLGVFMVGALVATMINVKFSWAPTIGDVTLNMQN
                     YIDMILPKILPAGIVGGVYWLLGKKNMTPTRAIFIVLIVCVALSALGVISK"
     gene            complement(12768..13658)
                     /locus_tag="U2K71_001805"
     CDS             complement(12768..13658)
                     /locus_tag="U2K71_001805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002364095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7315885.1"
                     /translation="MDIQWWQILLLTLYAGYQILDELQIYSSMSAPVFAGLYAGLVMG
                     DLNAGLIIGGSMQLTVLGVGTFGGASKIDANSGTILATAFSISLGMNPEQAIAAIAVP
                     VASLMIQLDILARFANTYFAHRIDRHVEDMNYQAIERNFLMGALPWSLSRMIPVFLAL
                     AFGGGLVENVVSVLNGNLKWLGDGLSVAGAVLPAVGFAILLRYLPVKKHLSYLILGFT
                     ITALLATLFSNVQLLGTSVASVVKDLPATFNSLPMLAVALIGLALAMISYKNDQNKDH
                     QPLASEPSHSVEGEIEDDEI"
     gene            complement(13671..14150)
                     /locus_tag="U2K71_001806"
                     /pseudo
     CDS             complement(13671..14150)
                     /locus_tag="U2K71_001806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632653.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system
                     mannose/fructose/N-acetylgalactosamine-transporter subunit
                     IIB"
     gene            complement(14326..15267)
                     /locus_tag="U2K71_001807"
     CDS             complement(14326..15267)
                     /locus_tag="U2K71_001807"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hexose kinase"
                     /protein_id="HEM7315886.1"
                     /translation="MITTVTLNPSIDIAYTIEELNLDKVNRVETVNKTAGGKGVNVAR
                     VLNLLGEEVIATGLIGGQHGEYLKNQLDQLGIMHQFVPIKGETRDSIAILHSSRQTEL
                     LERGPFITKKEQQTFTDHFEQLLEISDIMTISGSLPQGCPLDYYKQLVALADKKGKDI
                     LLDTSGKFLLEVLEGKEKPLLIKPNLEELGELIGQRICSSIQLEKIQSLLAHPIFEGV
                     PWVVVSLGERGAVAKVAQCFYEVIIPKISVVNPVGSGDATLAGSAHALSKRQSIEEIL
                     AFSMACGMANAQQEKTGWIDPMKVTMLMKHIQVKKIK"
     gene            complement(15264..16259)
                     /gene="lacD"
                     /locus_tag="U2K71_001808"
     CDS             complement(15264..16259)
                     /gene="lacD"
                     /locus_tag="U2K71_001808"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEM7315887.1"
                     /translation="MNEEKNRAMERLSTKDGIISALAIDQRGALKKMIKSLGKEPSED
                     QISEFKELVSAELTPYASAILLDPEYGLPAAKVRAKETGLLLAYEKTGYDATTPGRLP
                     DLLADWSSLRLKEQQADAVKFLLYYDVDEAPEINHLKHVFIERLGSECEGENRPFYLE
                     LVSYDAQISDSSSPEYAKVKPHKVIEMMKEFSDPKYKVDVLKVEVPVNMNYVNGYSTG
                     EFIHSKEEAAAFFKEQSEATPLPFIFLSGGVSTELFQQTLIFAHESGSTFNGVLCGRA
                     TWKDGVKAYVENGKDGAIHWLRTNGRENIETLNKVINETATPWFNKTKSKKVKMK"
     gene            complement(16252..17448)
                     /locus_tag="U2K71_001809"
     CDS             complement(16252..17448)
                     /locus_tag="U2K71_001809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM7315888.1"
                     /translation="MFELSKNKLKQIGAAITTQEIKQQPDLWKETIDRLMQSQENLAL
                     FFDQIKKEANGRRIRVIFTGAGTSQYIGDTLLPYLNEHGDNQFFIFHSFGTTDIVASP
                     KEFLFPNEPTLLVSFARSGNSPESVAAVQIANEQVETLFHLTITCAKEGMLAKNAQDD
                     KQNYLLLMPERANDAGFAMTGSFTCMLLSALWIFDQTAMEKKERYLEQLVKIGQDLLS
                     RDQEIQKLLPDFFERIAYLGSGSLAGATREAQLKILELTAGKVATIFDSSMGFRHGPK
                     SFLNSETLVVGFVSNQPYTRNYDLDILEEIKEDRVTANVIAIAQPGTIDFSGMTVLLE
                     DSVSKLPDAYLALAMIMTAQIIALYTSIKVANKPDTPSPTGTVNRVVKGVIIHKYETE
                     GEMTDE"
     gene            complement(17466..18194)
                     /locus_tag="U2K71_001810"
     CDS             complement(17466..18194)
                     /locus_tag="U2K71_001810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM7315889.1"
                     /translation="MESSNKGKSLYYQLVDLLKDRIETQMTPHDKLLSERELCQHYGV
                     SRTTVRLALQELENQGYIYRRHGKGTYVSDLSDKSSNLAGAYSFTEEMREIGRVPKTV
                     ILEFRQMEANKRIAKNLNIHLGETVFKLKRLRLADEEPLMVERSYLPAKLFMGLDLPL
                     LESKPLYDVFSQDYEQQVQLADEEFYASIVQKKDAVVLRIADGAPVLHLTRITYNVKN
                     EIIEYTDSVARADQFHYKIRHIRK"
     gene            complement(18635..19609)
                     /gene="lacD"
                     /locus_tag="U2K71_001811"
     CDS             complement(18635..19609)
                     /gene="lacD"
                     /locus_tag="U2K71_001811"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEM7315890.1"
                     /translation="MKKMSIEKQERLKRLTDEAGIISALAIDQRGALKKMINQYKEAT
                     SEDIIQFKQMVSQYLTPYASAILLDPEYGLPAAALREKECGLLLAYEKTGYDAKMPGR
                     LPDLLNSWSVKRLKEVGADACKFLLYYDVDEPVEINVQKQIVIERIGSECEAEGIPFF
                     LELLSYDATIGDPSSLDYAKVKPHKVIEAMREFSKERYQVDVLKVEVPVNMRFVEGYG
                     QDTCYSQAEASAYFYEQSKVTEQPFIFLSAGVSAALFQDTLRFAKQSGSTFNGVLCGR
                     ATWAKGVAPFVTEGEEKASSWMLEEGKQNIKELSTLLEETATSIYVNK"
     gene            complement(19606..20064)
                     /locus_tag="U2K71_001812"
     CDS             complement(19606..20064)
                     /locus_tag="U2K71_001812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM7315891.1"
                     /translation="MTIIHEENIFLQQPLDNQEEAFKFLAKQTFCLGIAHDEKEVYQK
                     LLEREKEGTTGIMSGFAIPHAKSQTINEPSLIIVTLKKGIEWNSLDRQSVDFIFALFI
                     PDSEAGTTHLKLLSLVARSLMRKEVTEQLRAAKSKIEIATILTKEIGEDI"
     gene            complement(20061..21524)
                     /locus_tag="U2K71_001813"
     CDS             complement(20061..21524)
                     /locus_tag="U2K71_001813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM7315892.1"
                     /translation="MGKYQLIAATGCPTGIAHTYMAQEALEQAARKKGITIKVETHGQ
                     IGIENELTAEEIKQADAVIIAADKDVHSERFVGKRVIDVSVSTGIKDAQRLIDEALAG
                     KGTVLADNEAADTLEPEAISGANFGRSIYKNLMNGVSHMLPFVVAGGVLIAISFAVWG
                     IYSSDPDHAQYNATAAMVKAVDDAAMGMMVPVLAAYIAEGIAKRPGLVVGFVGGIVAM
                     NGGTGFLGGILAGFLAGYFILFLQKLLIILPKQLDGLKAIVLYPVIGVAFIGIVMSLL
                     AGPMKAINEGMMSFLAGFEYSNPLVLGVIVGCMCAFDMGGPVNKAAYVTGTALLTQGN
                     TTFMAGASAACIAPPLITGFATLLFGKYFDTNDRNAGLVNFILGSTHITEGAIPFAAK
                     DPIRVLPTMMLGSSIAAVLTYLFGVQVPAPHGGFLVLPVVTGKIQWVTAILIGSIVGG
                     VILGLIQKKRVTDKDKKRDNKATNRTVIEEGRAGGLS"
     gene            complement(21506..22438)
                     /gene="pfkB"
                     /locus_tag="U2K71_001814"
     CDS             complement(21506..22438)
                     /gene="pfkB"
                     /locus_tag="U2K71_001814"
                     /EC_number="2.7.1.56"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-phosphofructokinase"
                     /protein_id="HEM7315893.1"
                     /translation="MSIYTCTMNLAIDLFIETDEMHPFMVNRTKEDDIQANGKGVNVS
                     LVLKMLDFPSTALGFSAGFTGKYIEDYLQQKQIQTEFIEVPGMTRINVFTQVNQTGEE
                     YKLVNQGPEIPQTAVHNFLNQIRALQAGDYLCVSGSLPRGLSPKVLIEISRICLIIDS
                     SDQEVMDCLPYRPFLLKPNEEELSSWFGRKMVTDEDYFVYGQRLVESGAENVLLSLGE
                     KGAILFTKDRVIRGNSPKGKVVNTACAGDAMLGTFLAGYMNRRSLDETLRKSIAAGSS
                     TAFRKGLTDFLDVEELSKQIKIKEERFERWENIN"
     gene            22580..>23156
                     /locus_tag="U2K71_001815"
     CDS             22580..>23156
                     /locus_tag="U2K71_001815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM7315894.1"
                     /translation="MNHKLSETEQYLWNFIEHLILEIPNYSIVKLSEQANVSTATIVR
                     TMKKKGYEGFTSFKHHLKEGENKNINFSFLDKVDKGIRRAILKNEQEVVRTINMLEIG
                     NIEDAIQRIKFADRVFVFARGFSEMIGQEMLVKLQLTGKNCQMHTDPEIIKSISRKLT
                     KKDSVIFVSLNGEKTNKNIKNKIYVFIFQFFK"
BASE COUNT         6956 a         4956 c         3888 g         7356 t
ORIGIN      
        1 tattttttag tataggttaa ttaagcttta gtaacgtttg ttgcttgagg tccgcgttgg
       61 ccttcttcaa catcgaaagt tactgcttgg ccttcttcta aagttttgaa tccgtcgcct
      121 tggatagctg agaaatgaac gaatacatcg tttccgtttt ctgcagtgat gaatccaaat
      181 cctttttctg agttaaacca ttttactgta cctgtttcca taataaaaat cctccttgtg
      241 tatcaacaca tattttgcaa ttacttgaat ggtgtatatc tggaagatgt ttatttgaaa
      301 caagctacca cagattaccg aacaaaaact actcctatag tatatcatac cgtttacaaa
      361 aaagctagag tttttcgact ttttttatta aaaaaagttt tctcagcgat tacataaagt
      421 gaaaaaccga actttttttt aaaataaacg acatattgtt cagttggtgt ttttttgctc
      481 tgatgttctt ttttaaacta aaaaaattat tctttctgac tacttatcgg ttctgtttca
      541 acggtcgagg ctggggaaga ttctttagta ggtctgatgt tctcatggta tttctgatag
      601 cctatgacag ttaccagaag aacaaggcaa accaatacga ccaacgagat tagtttttct
      661 tttttgctca tatctatacc tcataaataa caggatgatg tccatcatat ttctgagtat
      721 gagtaaaagc aaaagctgca gctgtagaat gtaagaaatg ccagcaactt tcataatcac
      781 aggtcggaca gtaccaaaag taaccaaatc ttggctgcaa agaccgttct tctgtttcag
      841 gaatcgcaga atcaagatct ggtgtctgta cataaacatt tttgtacaga cgtgtgcctg
      901 taataggaaa ccgctgtgtt tcttcagcaa aaactggctg acttgttaaa cttagggata
      961 gagacagcaa acaccataac agtctttttt tcataaagac ctccttatcg ataagttttt
     1021 cccaattaaa atcatatctc aagaacgccc gttattcaca ttttgttcat aaatgatgtt
     1081 ttttgtatca taatcgtcaa aaagaggtcg tgaccacgtc acaacctctt ttttaaacgg
     1141 tgttccagaa gtagcttctt cggaaataag ccgaaatttc acaaaaattt gagaaataat
     1201 ttttgtcaat ctcttcttat ttcttgaagc tgaccacttc tgtcacaacc tcttccttaa
     1261 acggtgttca aaagcttgca tctgcggtaa taagcccacc aaacaaaaaa tatgaagagc
     1321 tatttttgct tgctgttctt attacttgat gcagaacagc tttttcacaa cctctcatac
     1381 acggtgttta atcagctgac cttcggtatt agctcaaaaa acggcaaaat atgtgaaact
     1441 atttttccgt ttgtttctct aatactcagg tcaaaacact gatttcacaa cctcttttaa
     1501 taacgaagaa tcgctgtcag actcttttca tctggcgcat ctttttgatc caaaataaac
     1561 acttttccgc cattaatgat cacatcatct gctaatgcat tcaacacttg acgccgatca
     1621 tattctgttt caggatcttc accgaaacca tcagctaagt ttgacgtagc gataaataaa
     1681 tgtgacactt ttccttcttt cgcagctgga acgatatcaa ctaactgatc gatgaatttc
     1741 ttatctaaga gtttattgta ttcttttgat tcttgagctg cgagttcact tgacagcttc
     1801 ttcgcccctt cttcgatatc tctaaaggat agctgcgcag gagaagcaga aatcgcaacg
     1861 tctgcacaat aatagggatt tttagcaatt tttttgaaca tcgtctgatt ttctggtaga
     1921 gcaaataaat aaatcggcca ttgttctgga ttttcgaaag aatccttcaa gtacgtatca
     1981 actgcttggt aatagttgac ccaatcgatc tctacttctt catcttttgg atttacgcca
     2041 tggaaagtta ctccatctcg ggctccgcct cctccattag aagagaagtt caaaccgcca
     2101 cctgtcagtt cagatcccaa tgcagtaacg acatcaaccg gtgccccttc tggtagagat
     2161 acttctttta cttgtttgtt gctgacttca tacaatttca tggagtcgcg gttcaatccc
     2221 aataaataat aagaatagtt gaactgccca tttttgataa tagccagtaa ataaggaaga
     2281 tcacttacat agtattgatc gtctaccggg atactcaaac gatggatata ggtttggtca
     2341 gcagttataa tgaccgctac actcgtagtt gcactacgcc aaaacgaagg atctcctaac
     2401 aaattttcaa ttttttcttg gaacggggac cagtcatgtt cggaatattt tttctcaaaa
     2461 cgtttctttg cttctttcgc aaaatttttg aatttgatct gatctttttc cacatcttga
     2521 tgggcgacat gcgtattcag catgatcgtg acgaatggtc ctttagcgcc ttctgaagtc
     2581 aattgtgaca ataattcttt atcctttttt gccataaacg tacccctcct aaaaaaatca
     2641 ttcctttgtt tctatctaaa gtctaccaca acactatttt tccatacaat aaaacgtatc
     2701 taaaaaaata aaacggtttc tacggtttta aatttagcta tttttttaag ttatcttcat
     2761 aatttcctca attatttagc cccagtcttc aactattctt catgaaatgc tgataaacta
     2821 agaaaaagaa taaaagaggt ggatcttatg catttgatta tgattttcag tttattaagt
     2881 attatcagtt tatttgcttt cagcgcttcc cgcttattag atgcggaaac acagcctatt
     2941 cctgtaaaag tacgtaaaaa cgaccgctaa ccttccataa cgtgcgttct tatcctaaag
     3001 ttttttcaga aacattgaaa ctctctcccc tctctgataa aattttaatt tgtagcgaaa
     3061 caaattaatt acaaggagat cggatatgtc cataaaaaaa acacgtctag tcctattatt
     3121 ggctagtctc attctttgcc ttgcttcttt tctaccagct gttgctgtgc aagctgaaga
     3181 tactttcaaa gtaaatgcaa aagcagcctt tgctgtagat gcgcaaagcg gtaagattct
     3241 ttatgatcaa gatggagaaa aaaccatggg gatcgcttca atcacaaaaa tcatcggtct
     3301 ttacatcgtc cttgatcaag taaaagaagg gaaactatca tgggatgata aagtttcaat
     3361 ttcagattat gcagaaaatt tgagtgtcac cccagattta tcaaatgttc ctttgcataa
     3421 agaaaacacc tacaccgtta aagaattatt cgactctgca atcatccagt cagccaacgc
     3481 ttcaatggtt gccttggcag aaaaaatctc tggcagcgaa gcaaaattca ctgaacgaat
     3541 gaaagaacaa ttgaaagatt ggggtatcaa agatgcaacg atcgttaatg catcaggact
     3601 gaataactct tatcttggcg aaaaccgacc agaaggtact ggagaaaacg acgaaaatca
     3661 aatgtctgct caagatgtgg ctatcgttgc ccgtcatttg atcttggatt ttccagaaat
     3721 actggatgta tctagcacta cgacacaaat gtttggcgaa aacacgcaat caccagtaga
     3781 aatggtcaac tggaactgga tgctcccagg attcgtgaat tataaagaag gcgtggacgg
     3841 attaaaaact ggtacgactg aacttgcagg agcctgtttc gtcggtacga tcactaaaaa
     3901 cgatcaacga atcatcactg tcgtattgaa tgcagacggc catgcggaaa acccaagtgc
     3961 ccgtttcaat gaaacagctc gcttaatgga ttattgctac gacaattggt cagaaaaaga
     4021 gcttggaaaa gccaatgcaa gtatccctag tctaaaaacc attgatgtaa aagacggaaa
     4081 agaatcaaca gtgcccgttg tgcttaaaag tcctgtaaaa gtatgggtac ggaacgatat
     4141 ggatactgga aaattaacaa tcacaccaac tcttgacaaa aaacaagtca ctgacaatga
     4201 gatccaagca cctatggata aaggaacaaa aatcggtacc gcaacgatca ccttggcaga
     4261 agataaatta ggttatcttg aagacaacaa cgcgccatcc acagaaatca tcacggataa
     4321 aaccgtagaa aaagcaaata ttttcgttat tggctggcga cgcgtcgcag aatttttcgg
     4381 taacttgttt taattgaaca aaaagaaaaa agcatctttc catgtggaag atgttttttt
     4441 gctttttcta gtttaaataa cagctttttt catctatttt cgttatactg tataagaaaa
     4501 aggagttgaa tttatgaatc taaccagaga tctgattttt ttggttctct atcttattgg
     4561 ggtattctcc atcgtttatt ggcgaaaaca gaaaaaggat ccttccttgt tcttttacag
     4621 cagtacatgg acgtttgcgg taatcttgac ttattttgtc gtgttggaaa ttttttattc
     4681 gaccttttat tttttgattc ccttagtgtt tttcagtatc tttgcctttt cctatttttc
     4741 agaaaagcga aggctgttga atggtctttt attcaatatt ttcctcatta gttttggtat
     4801 ctacttgttt gtcttgctat acgaaacaca aaatattttt cttggcggac tgattgcctt
     4861 gatcacgatc ccgttactgc ttgtctttct attcggcatt tatggattga tcgtttttct
     4921 tttttggaac ggtgtcacag ttttacgtag agagtcgcat tcattagcta atcttttaac
     4981 gttgatacta gccatttttc ttacattgtt tcttgttttt gactttttct tgttaaaata
     5041 tttaccacaa tggataaatg ctttgttttt ctgcatccca ttgatcttga tctatctatt
     5101 tatcgtattc tacaactttt tgactgtctc attcttgtat cagttcaacc gccctcgata
     5161 caaccaagac ttcatcgttg tgttaggcgc cgggttgatc aatggagaaa cagtttctcc
     5221 tcttcttgcc aagcgaatca ataaggcgat tgctttttat tgggcacaaa gccgtgcgac
     5281 attaaatccg cctatcctat taatgtcggg tggacaggga gcagacgaaa aagtaccaga
     5341 agcaatggcg atgaaacaat atgccatgga acaaggaatt cctgaaagag atattcttgt
     5401 cgaaaccaat tcgacaacca ctttggagaa tatgcgctat tcaaaggaaa tcatggatca
     5461 gcaaatgaaa ggtccctatc gcgcgatctt cagttccaac aattaccaca ttttcagagc
     5521 tggtctctat gcacgacaag cgaaactgaa ggcaaatggc attggggcaa aaacagcttt
     5581 ctattacttg cctaacgcgt ttttaagaga atacatcgcc atcttgatga tgaacaaaaa
     5641 aagacatatg atcatttgcg gattgctttt cgcaggttcc gcattcttat cactgatcac
     5701 tttacttttc taaaaaaaca aggtgtgaca aaacatatgt cacaccttgt ttttttcttt
     5761 tattctcttg acgattggca agaatagtat tccttttttg acggctactt gctgtgcaga
     5821 ataaataccg ctgtttcctg aacacataaa gctgatcaag cagatcataa agaagtaagc
     5881 agcagcttgt gaaccaaata attcgattcc catcacaaaa caggcaatcg gtgtatttgt
     5941 cgcaccagaa aataccccga taaaacctaa tccagcaaga aaaggaacag aaacatggag
     6001 caaaggcgct agtactgcgc caagcgttgc accaatctca aatagtggtg tcacttctcc
     6061 cccttggtac cccgcaccta aagacaagac cgtgaagaat aatttcccaa taaaatcata
     6121 agcatgtgcc tgtccattaa atgattcctg cagtaacgga agactcaacc caaggtaacg
     6181 ttgtgtttgc aaaatcaacg ctgcggctac aactactgct ccacctatcg tatttcgcca
     6241 aacaggatga ggaatccatt gcgcataccg cgcttttaaa aagacgatcg aacggctgaa
     6301 tacccaacca atcaaaccaa aagaaatccc agcaacaaaa actttgaaga acagcaacat
     6361 actccattca ggaatcacac ccatttggta atgtagatga gaaacaccat agctttctgt
     6421 catgaaattt gcaaaaagcg cagcaaagaa gctaggaaat attgcttctg ttcggacttt
     6481 accgattgcc aacacttcta atccaaataa tgttccagct aaaggtgtgc caaatacaga
     6541 actaaaaccc gcactgattc cactgataac caaaatctcc cgttctgctt tatccaatcg
     6601 gaataaatgc gctatattat cagctaatgc tccccccatt tgaactgctg ttccttcacg
     6661 accaacagat cccccaaata aatgggtagt gatggtccca aacaaggtca atggaatgag
     6721 ccgcaaaggg atcttctctt ctcctccatt cccttgatcg atcactaagt tattcccacg
     6781 actagaaaga ccgccatgat acgcataaag ataagcaaaa agcgcgccgc tgactggcaa
     6841 tagataaagt agccaaggat gtgctagtcg gatatctgtc acgaacgtca ggctatttaa
     6901 gaaaaaagca gacaagctcc ccatgaatac tccgagaatc gctgtgacca ccagccactt
     6961 caacatgtac atcaaaatac gcactggact cggtaattgt ttttccataa aaaattcctc
     7021 ctcaaataaa aaaacgccta ctaaagaaag gggctggccc cagactttag taggcgtcat
     7081 tagctatctt acgttagcat ttaaaggcga acaccatcac ctattcactt ccattaaaag
     7141 atagcatatt tttctgtaaa aaacaataaa attatcgtta tttctttaca cgttcagcaa
     7201 attcatagaa aggagccaat ttcgttaact gttgattaca ttgatcacta tcatgacaaa
     7261 tataattccc ctttttcgta tacgtgccat cagaaccggc tttcgttgta gaaagaaaga
     7321 gcgagacatt cgatgttttc tgacagatcg cacagattcc cttattaatc gttggtgata
     7381 aagtaccgct gatccctttt aattttccat cttcgtaata aaccatgaat tttttttgcg
     7441 tccctggatc attccaaccg atgtacgtat gtgcttgcaa gtcgatttca ttccaagcgg
     7501 gttgtttgag ttttttcact tttcggaata atttctcgat ttgtggtttg ctcggctgca
     7561 taaacggctg gacgatttgc ttcagctcct ccaatgctcg ttctgcttgc gtttttgtca
     7621 gttttacatc taaaactgtc gttaaaaacg tatcgatttc tgatggttcg taacctaata
     7681 catcgcgaat cttttcagct gctagagcct gaaccgtttg taccgttttt gagtcattga
     7741 cagatgagta cgcatttaat aactgctcta cttctctttt tataaaaaaa tattgatatg
     7801 gtttcattgt ttgttccatg attttaaagt cctttcttct aaccaaagtg catactttgt
     7861 agaaaaaagt gatcttctgc aaagtatgtg ttacctgaag atcccggact cttttaaata
     7921 agatacgttt tgcatcttat catcaccccc tttaatgttc attcttctat gtacatgatt
     7981 cattcgataa ataaaagtaa accgtatctt gagcagatag tcaactgcaa acagaaaaag
     8041 gactgtgaca aaagtcgtgt caaagtccct ttctacaaac aaacggtgtt ccagaagtag
     8101 cttcttcaaa aaataagccg gaatttcaca aaaatttgag aagcaatttt cgtaaattcc
     8161 ttcttatttc ttgaagctga cccacttctg tcacaacctc aatatggtct attattggtt
     8221 ttacctgtta aatcgaggat tcttttccga agtaacgttg ccatctctta cgcatacttg
     8281 ctgacatagg ctgagcggct tctttgactg cgcagtattt gtctacgaat gttacgatat
     8341 atccttcttt gtacttaggc ggtgcaatcg ttgcgcccca catgtgcttc aaaatgatat
     8401 ctcgctcaag atccgacaaa tcggtgatct tttctgcatt ctttacagcg attcttggat
     8461 ggatatacgc atgtgtgcct tcatcaaatt tcgtcgtacg ccaatcatag tagaacagat
     8521 catgcaaaag tccagcacgc gctgttgctt tagcattgcc accccatttt tttgcgagca
     8581 gataactata gtaagaaaca ctgatggaat gctccaaacg agtggaatga acatgctgtg
     8641 tgtaattcgc taattttttc actgcttctg tttctaaaag atcttctaca taagacatat
     8701 attcttcgtc ttcacgccat ttttctgtca taattatcga tctgccacct ttttgaattt
     8761 cattttcttc tgaaattgat ctaagtatac caccagtgtt cggattattc tagtcgcctt
     8821 ggataaaata gccaaagctt aatagttcct taatcataaa gaaaaatgat aaagaacaat
     8881 ccctgccgca accccaacat tcaacgattc cgcatttcct ttcatcggga tatatacgtt
     8941 ctgatcagtc aaagacaaga tttcctgact taccccctga ccttcgttcc ccatgattat
     9001 tgcgaagtta tctgattttt ctactttgtc taaggcgtgt gcttcttcat ttaattctgt
     9061 gccatacact gtaaaccctt tgtctttcat taaaggaatc acttctgtca aatctttttg
     9121 caacacaggc aaatgataat tgcttccctg catgctgcgc agcacctttg tactataaat
     9181 atctgccgtt cctttgccta ataaaacacc agccatatca aatgcatctg ccgtacggat
     9241 catcgtccct acatttccag gatcttgtac attatctaat aacagccatc ctcctgaaaa
     9301 atctggcgtg ccttcagccg ttaacgccat cactgctaag attccttgtg gtgtaggtag
     9361 atccgataca gcatccatga cctcatccga aactagaaaa gtagaaatct ctttgtttgc
     9421 ggccagccag tctccccatt ctttcatgcc tcgctgtgtc acaaaaagtt cttttatttc
     9481 tgcatcagac gacgcagctt cttcaatcag atggaaccct tccaaaagat attcttcctt
     9541 ttcttcccga tatttctttt tatgtagttt ttttaattct ttgattcttg gattttttgc
     9601 tgattggatc tctttcacat tcctcacctc cccatgatta tatcatggaa taggtgcttg
     9661 agcttgtttc atcctaaaaa aatcggtatc attaatttat aaaataggaa agaaaggtga
     9721 cgaacatgcg caaagttaaa atgaacgtcc aaggacgcgt ccaaggcgta ggatttcgct
     9781 acatgaccaa gatggtcgct gatcaactag gcgtgactgg aacggtaaaa aacgaagatg
     9841 acggctcagt cacgatcgaa gcaatcggca atgacgacat catccagaaa tttattgaag
     9901 aagtcaagaa atctccgagc ccaagtggac gagtgcagta tgtggatgtc caagaagacc
     9961 cgatgattga ggaacgaaag aaatttgatg tggtgggata aggttgtgaa aaagctggtc
    10021 tgcatcaagc attaaggaca ggttgtgaaa aatagctctc catatttttc aacaggctgg
    10081 acttaatgcc gtagatgcaa gcttttgaac accgtcaata aggttgtgag aaagtcgctt
    10141 agttttgagt agtaaggaga attttgtgaa aatggctttc cacatttttg cataaaattt
    10201 gacttaatac cgagaaactg acttttgaac accgtcgata aggttgtttt tgagacgctt
    10261 tgcctcaagt attaaagata ggtagtaaaa aagctgatct ataacttgca ataaatttgc
    10321 ttaacccata ttttccagac agaacaaaaa agtttttagt tttttaaata agaaagaaac
    10381 acttggaatc actcttttct ttccaagtat ttctttcttg attttttgtc tttttttcaa
    10441 aatttttcgc tacgtaaaag aacgaatatt tatatgaaat ttctatgaag ttgattgaat
    10501 tccatgcgct tttttagtat agttaagttt gtgtaaaaat tatagaagag gaagtattct
    10561 atgaataaat ggaaaaactg gttattggga tccggacttg tcagtatcct gctcttttta
    10621 tctggctgtg tgcggatgga ttcaaacgga aaccctgaca cttcgggaat cgtttatcga
    10681 gtactcgtcc atcctatggg ccaagcgatt acatatctag ttgagaattt caattggtct
    10741 tatggctggg cagttattgc aatgactgtg atcgtccgta tcatcatctt gccactaggt
    10801 attagtcaat cgaaaaaaac aatgatccaa tctgaaaaaa tgcaagcgtt aaaaccacaa
    10861 gttgaagcag cccaacaaaa attgaaagct gcaacaacac gtgaagaaca aatggcagca
    10921 caagcagaaa tgcagcaagt ataccgcgaa aacggattga gtatgaccgg cggaatcggc
    10981 tgtctgcctc tattgatcca aatgccgatc ttctctgcct tgtactttac ggcacgttat
    11041 acagaaggta ttcgggaatc atctttctac ggcatcgatt taggctcacc aagtatggta
    11101 cttgttgtca ttgcaggggt cgcctatctg cttcaaggat acatctcaac gatcggtatt
    11161 cctgaagaac agaaaaagac gatgcgtacg atgctaatcg tctctcctgc tatgatcgtc
    11221 ttcatgtcga tcagtgcgcc agccggtgtt acactttact gggtagtcgg tggtgtgttc
    11281 agctgtctgc aaacattcat cacaaacgtt atgatgaaac cacgtttgaa agcacaagtg
    11341 gcagaagagt tgaaacaaaa cccgccaaaa caagtcgtca ctccaagaaa agacgtgaca
    11401 gaatcagcaa agccaactaa caacaaacaa gcaacaaaac aaatcaaaca accaaaaaac
    11461 agtacaaaca acaaaggccg caacgcagga aagcagcaga agcggaaata aaaacatagg
    11521 acaaaaaaga tgagagctaa gccctcatct tttttgttac tcttcctctt ctgctattcc
    11581 attaattaat tcgttgacag agacaatgct ttcacatcct gcacgaacaa actcgggctc
    11641 tgaaccaatc atttgtgagt tgataaattc aatgaccatc ggaagattca tcccagcata
    11701 aagagtaatc tgttgtcctt ccattattag tttagaaatg atatttgcag gtgtaccacc
    11761 taaaagatcg catagtacca caaaatcatt taacgaatct gtgacagtga gaaacttttg
    11821 tttgtatgtt tcagtgccat cttctggtaa taaaggtaac gtgtagatat cctcttgagg
    11881 ccccatgatc atttctgtac tttttttcaa ttcctcacaa aattttccgt gactaatcaa
    11941 taccaattgt ctactcattt ttcgctctcc cttatttcga aatcacaccc aatgcagata
    12001 aagcaacaca cacaattaat acgataaaaa tcgctcgagt aggagtcatg tttttctttc
    12061 ctaataacca ataaacacca ccgacgatcc cagcaggtaa aattttaggt aagatcatat
    12121 cgatatagtt ttgcatattc aatgtcacat cgccaatcgt tggtgcccat gaaaatttaa
    12181 cattgatcat tgtcgcaact aatgccccta ccatgaatac ccctaataaa gtggctgcgt
    12241 ccgttaatgc agttaagcga gattgcatag tagtaactaa ggaaattcct tctttgtatg
    12301 caaattcaag ttgcttccaa cgaaaaacca tgacaacgat ctgagcaaaa atccatataa
    12361 acacacctat cggatttcca tcagttgcca tattcgccgc caaagcccca aaaatcgttg
    12421 gaatcaaagc agcaaatacg gaatctccaa tcgctgcaaa ggaacccatc aatccagctt
    12481 tcagccccga tacggtttcc tttgaagcaa ttccctcttt ttcttccatt gcaagatcaa
    12541 tcccagtaat gattgtatta aaaaaattag aagtattaaa aaattgcgta tgtgttttca
    12601 tcatctcttt taattctggt gtattgtctc catatatttt tcttagttgt ggcaagatag
    12661 tatataaata gccagaaccc tgcattctct catagttcca acctaattga aaagtgaaca
    12721 aacttcttcg attgatttgc ttaaaatctt cttttgttaa tttgtaatta gatttcgtca
    12781 tcttcgattt ccccctcaac agaatgtgat ggctcactag ctaatggttg atggtctttg
    12841 ttttgatcat tcttataact gatcattgct agtgctagac caatcaatgc tacagcaagc
    12901 atcggcagcg aattgaaagt tgccggaaga tctttaacaa cgcttgccac acttgttcct
    12961 aataattgaa cattagagaa taacgttgct aataatgccg tgatcgtaaa tcctaaaata
    13021 agataagata gatgtttttt tactggtaaa tagcgcagaa gaatggcaaa cccaacagct
    13081 ggtaaaacag ctcctgctac tgaaagtcca tcgcctagcc attttagatt cccgtttaaa
    13141 acagaaacaa cattttcaac taatccacca ccaaatgcta aagctaaaaa tacaggaatc
    13201 atacgagaga gcgaccaagg taaagctccc attaagaaat tgcgttcgat tgcttgataa
    13261 ttcatgtctt ctacgtgacg gtcaatacga tgggcaaagt atgtgttagc gaatctagct
    13321 aaaatatcca attggatcat taagcttgct acaggcactg caatagcagc aatcgcttgt
    13381 tctggattca ttccaagaga aatcgagaag gcagttgcta aaatcgtccc agagttggca
    13441 tctatttttg atgcgcctcc aaacgtcccc actcctaaaa ctgtcaactg catactgcca
    13501 ccaataatca aacctgcgtt taaatcaccc attactaaac ctgcatataa ccctgcaaac
    13561 actggcgcac tcattgaaga atagatctgt aattcatcta aaatttgata acctgcatat
    13621 aatgttaata ataagatctg ccaccattga atatccatgg atcattcctc ctatttgatt
    13681 aaaagattca taaagtcttc tgctttatcg tttggtacca tttgagcaat taaatgtata
    13741 ccaagctgat ctaatttttt aaatacttcg atatctgcat ctaccacatt gattgatttt
    13801 gtaatggacc gtgtttcatt cgtttgtgac atattcccca catttacttc tttgatagga
    13861 actccttttt ctactaaaga taagattcga tctggctttt ttgctacgat caatactcgt
    13921 tgagagtcat atttgccagc taaaatattt gttgctgctt tttcgattgg caaaatgctt
    13981 aattttaccc ctgctggagt cgctaacttc aatccacttt tttcaattgt gtttgtggct
    14041 acctcatcat ccacgaccat gattcgacta atattcagtt tcgttgtcca aagatttgct
    14101 acttatccat gtatcaaacg tccatcgatt cttacaccaa taattgccat catgcattct
    14161 ccttatctat tttttctatt tattgttcaa ttaaaactgt gacatcatca aatatccctt
    14221 ctgtttcaaa gataacgttt tcattttttc tttcttatat ttcattttta agctaagaac
    14281 aaattccttc ccaactaaag aaaacgcttt cttttatatt taagttcatt tgattttctt
    14341 aacttgaatg tgtttcatca acatcgtaac tttcataggg tcgatccagc cagttttctc
    14401 ttgttgcgcg tttgccatac cacaagccat agaaaatgcg agaatctctt cgattgattg
    14461 cctttttgat aacgcatggg ccgatcctgc aagggtcgcg tcacctgaac caacaggatt
    14521 aacaactgat attttaggaa taatgacttc gtaaaaacac tgagcaactt ttgccacagc
    14581 acctctctct cctaaagaaa caacaaccca tggcactcct tcaaatattg gatgagctaa
    14641 taaggactga attttttcta attgaatcga agaacagata cgttggccaa tcagttctcc
    14701 taattcttcc agattgggct tgataagtag tggcttctcc ttaccttcca atacttcaag
    14761 caaaaattta ccagaagtat ctagtaatat gtctttccct tttttatcag ctaaagcaac
    14821 caattgctta tagtaatcaa gagggcatcc ttggggtaaa ctacctgaga tcgtcataat
    14881 atctgatatt tcgaggagct gctcgaaatg atcggtaaat gtctgttgtt ctttctttgt
    14941 tatgaagggg cctcgttcca aaagctctgt ttgtctcgaa ctatgcagga tagcaataga
    15001 atcccttgtc tctcctttaa ttggaacaaa ctgatgcatg atccctaact gatctagctg
    15061 gttctttaga tattcaccgt gctggccacc tattaaacca gtagctatca cctcttctcc
    15121 taataaattt agaaccctcg ccacattcac tccttttcct cccgctgttt tattgactgt
    15181 ttcaactcga ttgactttgt cgaggtttaa ttcttcaatt gtatacgcga tatcgatgga
    15241 aggattcaag gtaactgtcg taatcatttc atcttcacct ttttgctttt tgttttattg
    15301 aaccaagggg tagcggtttc atttattact ttatttaaag tctcgatgtt ttctctccca
    15361 tttgttctta accaatgaat agctccgtct tttccatttt cgacgtatgc tttgacccca
    15421 tctttccatg tcgcacgtcc acacaataca ccattgaagg tggaacccga ttcatgagca
    15481 aaaatcagtg tttgttgaaa taattctgtt gaaacacccc cactcaaaaa aatgaatggt
    15541 aatggagtcg cttcactttg ctccttaaag aaggcagcag cctcttcttt gctgtgaata
    15601 aattcacctg tggaatagcc attcacataa ttcatattga ctggtacttc tactttcaac
    15661 acatcaactt tatattttgg atcagaaaat tctttcatca tctcaataac tttgtggggc
    15721 ttgactttcg catactctgg agaagaacta tcgcttattt gtgcgtcata agaaactaac
    15781 tccaaataga agggacgatt ttctccttcg cactcactac ctaaacgttc gatgaacaca
    15841 tgcttcaaat gattgatttc gggtgcttca tcaacatcgt agtaaagtaa gaattttact
    15901 gcatccgctt gctgttcttt caatctgaga gaagaccaat ccgctaataa atcaggtaga
    15961 cgaccaggcg tagttgcgtc atagccggtt ttctcataag ctaacaataa ccccgtctct
    16021 tttgctctga cttttgctgc tggcaatcca tattccggat ccaataaaat agcagaagca
    16081 tagggtgtca gctcagctga tactagttct ttgaattcac tgatttggtc ttcacttggc
    16141 tcttttccca acgatttgat catttttttt agagcaccac gctgatcgat cgctaatgca
    16201 ctaataatcc catcttttgt tgatagtctc tccattgccc tatttttttc ttcattcatc
    16261 tgtcatttct ccttcggttt catacttatg aataatgacg cccttgacta ctcgattgac
    16321 cgtgcctgtt ggagatggtg tatctggttt attagctact ttgattgaag tatagagtgc
    16381 aatgatttgt gcagtcataa tcatcgctaa tgcaagatag gcatcgggca atttactcac
    16441 cgagtcttcc aatagcacag tcatacctga gaaatcaata gtacccggtt gcgcaatcgc
    16501 aatgacattg gcagtgaccc gatcttcttt tatttcctct aaaatatcta agtcataatt
    16561 tcgcgtatat ggttgattgc tcacaaaacc tacaacgagt gtctcgctat ttaaaaaaga
    16621 ctttggtcca tgacgaaacc ccatcgaaga atcaaaaatt gtagcaactt tacctgctgt
    16681 taattctaaa atttttaatt gtgcttctct tgtcgcacct gccaaacttc ctgaacccag
    16741 ataagcgatc cgttcaaaaa aatcaggtaa taacttttga atttcttgat ctcttgaaag
    16801 aagatcttga ccgattttaa ctaattgttc aagatatctt tcttttttct ccattgctgt
    16861 ttgatcaaag atccataaag cactcaataa catacacgta aaacttcctg tcatagcaaa
    16921 acctgcatcg tttgctctct ctggcatcaa taacagataa ttctgtttat catcctgtgc
    16981 gttttttgcc aacatcccct ctttagcaca agtaatcgtc aaatgaaaca atgtttcaac
    17041 ttgttcattt gcaatttgca cagccgctac actttctgga ctgttgccac tgcgagcaaa
    17101 tgaaactaat aaagtcggtt catttggaaa caaaaattct tttggtgaag ctacaatatc
    17161 tgtcgtgcca aaactgtgga agataaaaaa ctgattgtct ccatgttcat ttagataggg
    17221 taagagcgta tccccaatat attgagacgt tcctgcaccc gtaaaaataa cacgtattct
    17281 tcttccgttt gcttcttttt ttatttgatc aaaaaataga gctaaatttt cttggctttg
    17341 catcaatcta tcgattgttt ctttccagag atctggttgt tgttttattt cttgtgtcgt
    17401 gattgctgca cctatttgtt ttaatttgtt cttcgatagc tcaaacatgt tgttctccct
    17461 ctcaatcatt ttcggatatg ccgaatttta taatgaaact ggtccgctct tgctacacta
    17521 tctgtatatt cgataatctc atttttcacg ttataggtta tcctcgtcag atgtaagacg
    17581 ggagcgccat cagcgattcg tagcacaaca gcatcttttt tttgcacgat acttgcataa
    17641 aactcttcat cagctaattg aacttgttgt tcataatcct gtgaaaacac atcatacaac
    17701 ggtttacttt caagtaacgg caaatcaagt cccataaata gtttcgccgg tagataagaa
    17761 cgctcaacca tcaatggctc ttcatctgcc aaacgcaatc ttttaagttt aaatacagtt
    17821 tcacctaaat ggatgttcaa attcttagca attctcttat tagcttccat ttgtctaaat
    17881 tctaaaatga ccgtcttagg gacgcgccct atttcgcgca tttcttctgt aaagctatat
    17941 gctccagcta agttcgatga cttatcagac aaatcagaga cataggtacc ttttccatgt
    18001 cttcggtaaa tataaccttg attttccaat tcctgtaaag ccagacgtac ggtggtacga
    18061 ctcaccccat agtgttgaca aagttctctt tcagataaca gcttatcatg cggagtcatc
    18121 tgtgtttcga tacgatcctt caataaatca actagttgat aatacaatga ttttccttta
    18181 tttgagctct ccataaaaat cacctcttct ttactggtta taaccacaac acaagaatag
    18241 tgaattatta atcatttgtc aaaggttttt tagaccaaaa aagctattat aacagctctt
    18301 taaagcttta tatcttgaat agacaaagat acctcaaaaa taccagttac caataaaaga
    18361 acagaaaaaa agacgaacca cataagcgat ccgtcaaaat ggtctaactt atcgagcagc
    18421 gtgatccaca ttaatcccta tgatattcct acaactatcg ttttattact tccaatatca
    18481 tttacaacaa ctaaaaatag cttattaatg acaaatgtta ttattagttt tagataactt
    18541 ttgcagtttt atactttttt tctataaaat ttgacaaaca tttttttaaa taactaatta
    18601 ttttttgaat ctgtcactca gacaatcctt tctcttactt gttaacataa atagaagttg
    18661 ctgtttcttc taatagtgta cttaattctt taatattttg ttttccttct tctaacatcc
    18721 aactactcgc tttttcttcg ccttcggtaa caaaaggtgc tactcccttc gcccatgttg
    18781 ctcgcccaca taagacacca ttgaatgtcg aacctgattg ttttgcaaaa cgcaatgtat
    18841 cttgaaatag tgcagcgctg actcccgcac ttaaaaaaat aaacggttgt tcagtcactt
    18901 tcgattgttc atagaaatac gcggatgcct ctgcttgact ataacaagta tcttgcccat
    18961 atccttccac aaatctcata ttcactggta cttctacttt caatacatcc acttgataac
    19021 gttcttttga aaattctcgc atggcttcaa tcactttatg cggttttact tttgcatagt
    19081 caagagaaga cggatcgcca atagttgcat cgtaagaaag gagctctaaa aagaaaggta
    19141 tgccttctgc ttcgcattct gaaccaatcc gttctataac tatttgtttt tgcacattaa
    19201 tctctactgg ttcatcaacg tcatagtaca ataaaaattt acaagcatcc gcacccactt
    19261 cttttaacct tttcactgac caactattca gtaaatctgg tagtcggcct ggcattttcg
    19321 catcatagcc ggttttttca taagctagca acaaaccaca ttctttctct cgaagcgctg
    19381 ctgcaggcag accatattct ggatcgagta aaatagcgga agcatacgga gtcaggtact
    19441 gagaaaccat ctgtttaaat tggatgatat cttctgatgt agcttcttta tattggttga
    19501 tcattttctt caaagcacca cgttggtcga tagctagcgc gctaataatt ccagcttcat
    19561 ccgtcaatcg cttcaaacgt tcctgttttt ctatactcat tttcttcata tatcttcacc
    19621 tatttcttta gttaatattg tagctatttc tatttttgat tttgcagctc tcaactgttc
    19681 tgtaacctct tttcgcatca atgatcgtgc aacgagagac aataatttca aatgagtcgt
    19741 tcctgcttct gaatctggga taaataacgc aaaaataaaa tcaactgact gtctatctaa
    19801 actgttccat tcaattcctt ttttcagtgt gacgataatc aaactaggtt cattgattgt
    19861 ttgagacttc gcatgaggga tagcaaaacc tgacattatt cctgtagtac cttctttttc
    19921 tctttccaga agtttttgat atacttcttt ttcgtcatga gcgattccta aacagaatgt
    19981 ttgctttgct aaaaacttaa atgcttcttc ttgattatct aatggctgtt gtaaaaaaat
    20041 gttctcttca tgaatgattg tcatgataaa cctcctgctc ttccttcttc gataactgta
    20101 cgatttgttg ctttattatc cctcttcttg tctttatcag ttactctttt cttttggatc
    20161 aaacctagta tcactccacc aacgatcgaa ccaataagaa tcgctgtaac ccattggatt
    20221 tttcccgtca ctacaggtaa cactaaaaaa ccgccatgtg gggctggtac ttggacacca
    20281 aacagatagg tcaagacagc agcaattgat gagcctaaca tcatcgttgg taatactcgg
    20341 atcgggtctt tcgcagcaaa cggaatagct ccttccgtaa tatgagttga tcctaaaata
    20401 aaattaacca aacctgcatt acgatcatta gtatcaaaat atttaccaaa caataaagta
    20461 gcaaaaccag taatcaacgg tggtgcaatg caagctgctg acgcaccagc cataaaagtc
    20521 gtgtttcctt gtgtcaaaag agctgttcct gttacatatg ccgccttatt aaccggtcct
    20581 cccatatcaa acgcacacat acagccaaca atcacgccaa gaacaagtgg attcgaatat
    20641 tcaaatccag ctaaaaagct catcattcct tcatttatag ccttcattgg acctgccagc
    20701 aaagacatca cgatgccaat aaatgctaca ccaataactg gatataagac gattgccttc
    20761 aagccatcta gttgtttagg taaaattatc agtagctttt gcaaaaataa gataaaataa
    20821 cctgctaaga acccagctaa aatacctcct aaaaatccag taccgccatt catggctacg
    20881 attccaccaa caaatccaac gactaaccct ggacgtttcg cgatcccttc tgcaatataa
    20941 gctgctaaaa ctggaaccat cattcccatt gctgcatcgt caactgcttt taccattgcc
    21001 gctgtagcat tgtattgtgc atgatctgga tcagaagaat agattcccca aacggcaaat
    21061 gaaatagcaa tcaatactcc gccagctacc acgaaaggca acatatgaga gacaccgttc
    21121 atcaaatttt tatagatgct acgaccaaaa ttagcaccac ttatcgcttc tggttctaaa
    21181 gtatcagcag cttcattatc tgctaagaca gtaccttttc ctgccaatgc ttcgtcaatc
    21241 agccgttgtg catccttgat tcctgtactg acagaaacat caatgactcg ttttcccaca
    21301 aatctttctg aatggacatc tttgtctgcg gcaataatga cagcatctgc ctgtttgatt
    21361 tcttcagccg ttaattcatt ctcgatccca atttgtccat gggtctccac tttgatcgtg
    21421 atgccttttt tcctagctgc ttgctctaat gcctcttgcg ccatgtaggt gtgcgcaatt
    21481 cctgtcggac aaccggttgc tgcaattagt tgatattttc ccatctttcg aacctctctt
    21541 ccttgatttt gatttgttta cttaattcct ccacatccaa aaaatcagtc agtcctttac
    21601 gaaaagcagt agagctgcct gctgctatgc ttttacgtaa cgtttcatca agcgatcgtc
    21661 gattcatgta tcctgctaaa aaagtgccta acatggcatc acctgcacat gctgtattga
    21721 caacttttcc ttttggcgag tttcctcgaa tcaccctgtc tttcgtaaaa agaatcgctc
    21781 ctttctcacc gagggataac aaaacatttt ctgcgcctga ctctactagt cgttgtccat
    21841 aaacaaagta atcttcatct gtaaccattt ttcgtccgaa ccaagaagaa agttcttcct
    21901 catttggttt cagcaaaaac ggtcgatagg gtaaacaatc catgacttct tgatcactac
    21961 tatcaataat caaacaaatc cgactgattt ctattaaaac ctttggagac agccctcttg
    22021 gtaaactccc agaaacacaa agataatccc ctgcttgtaa tgctcttatt tgatttaaaa
    22081 aattatgaac agctgtctgc ggtatttcag gtccttgatt cactaatttg tattcttctc
    22141 ccgtttgatt gacttgtgta aatacattga tacgcgtcat accaggcact tcaataaact
    22201 ctgtttggat ttgtttctgc tgtaaatagt cttctatata ctttcctgtg aacccagcag
    22261 aaaaacctaa tgccgtactt gggaaatcta gcatctttaa tactagtgac acattcaccc
    22321 cttttccgtt tgcctgaatg tcatcctctt ttgtgcggtt taccataaaa ggatgcatct
    22381 catctgtttc aataaataga tcaattgcca aattcatcgt gcatgtgtag atagacataa
    22441 ccaacgacct ccttgattct attatgaaat acgcgaaata gaaaatgttt tcttaatcgt
    22501 cccaacacaa agaaaacctt tacatttata aactcgtcgt gcttttttta actaactttt
    22561 ggtgaaagaa ggaaagaata tgaatcacaa attaagtgaa acagagcagt acctctggaa
    22621 ctttatcgaa cacctcatcc tagagatccc taactattcc atcgtgaaac taagtgaaca
    22681 agcaaatgtc tcaacagcca ccattgtgcg gacaatgaaa aagaaaggct atgaagggtt
    22741 tacttcgttt aagcatcatc taaaagaagg agaaaacaag aacatcaatt tttcattttt
    22801 agataaagta gacaaaggaa tccgccgagc cattctaaaa aatgaacaag aagtggtacg
    22861 tacaatcaat atgttggaaa tcgggaacat tgaagatgca atacaaagaa tcaaatttgc
    22921 tgaccgggtt tttgtctttg cccgaggatt ttcagaaatg attggccaag aaatgctggt
    22981 aaaacttcaa ttaacgggaa aaaattgtca gatgcatact gatccagaaa ttatcaaaag
    23041 catcagtcgg aaactaacta agaaagacag cgttattttt gtttctttga acggtgagaa
    23101 aacaaataaa aacatcaaaa ataagattta tgtctttatt tttcaatttt ttaaat
//
LOCUS       DASHHB010000040        22826 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.041, whole genome shotgun sequence.
ACCESSION   DASHHB010000040 DASHHB010000000
VERSION     DASHHB010000040.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22826)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22826)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22826
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.041"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            259..744
                     /locus_tag="U2K71_001816"
     CDS             259..744
                     /locus_tag="U2K71_001816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dehydratase"
                     /protein_id="HEM7315895.1"
                     /translation="MNIGKPRKLGKTINEIEEGDSLSLTESIEDNQLLLYLGLTNDAN
                     PLYIQHDFAQKTEYQQPIVPSVMLMGIITSAISKHLPGPGSHVVNFSVNFIEPVFHYE
                     TLTFQFEVIKVDKMKDVVTISVEAENTNEDRVLDAVVMVQPPQVPELEIAIESEGELN
                     E"
     gene            737..1420
                     /locus_tag="U2K71_001817"
     CDS             737..1420
                     /locus_tag="U2K71_001817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HEM7315896.1"
                     /translation="MNNPSTSMSGINRFYAKVYGFLGMGLALSAVTSYLALWVFPLQL
                     ATFINNFPLGFVGLWIVELILVMVLGVKAQKNPSLAIGGFIAYSLLNGITLAVTLAYY
                     DIGTVTQAFLTATLMFMGMSVVGVTTKRDLSGVGRAGQAALIGVIIALVLNIFILRSS
                     VVELFISLLLVVIFAGITAYDNQSIRNIYVQTNGTAHSGIAVFMALKLYLDFINLFLA
                     FLRIFGRNN"
     gene            1546..4191
                     /gene="polA"
                     /locus_tag="U2K71_001818"
     CDS             1546..4191
                     /gene="polA"
                     /locus_tag="U2K71_001818"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HEM7315897.1"
                     /translation="MTKNKLLLVDGNSVAFRAFFALHNSLERFKNRNGLHTNAIYAFN
                     TMFENVMQKEQPTHVLVAFDAGKTTFRTEMYAEYKGGRSKTPGEFKEQMPYIRDLLTG
                     LGVQYYELPNYEADDIIGTLAEKVDKDQFDVVVLSGDRDLTQLASKEVKVDITVKGVS
                     DIESYTPEHVAEKYDGLTPKQIIDMKGLAGDASDNIPGVTKIGEKTAIKLLKQYGSVE
                     GIYEHIDEMKQSKMKENLINDKEQAFLSKKLATINTSAPVDVSIDSLKYEGKNLDKLV
                     PFYKEMDFNQFLSKLNIVEEEVKMDDILFEVVHEFKEEMFTTDMALYVEMMGDNYHTE
                     EIVGVAWGTEKKIYVTNDLSVFENRAFHSWITDPTRLKKVYDAKRTYVALNRYTGKTK
                     GIDFDVLLGAYLLDTNEKSTDIEGIAAHYGYNDIQSDEAVYGKGAKKGLPEEEELFFA
                     HLARKVAAINALTPKLSQELAEKNQEDLFRKMELPLSIILAEMEISGIKVDATRLQEM
                     KGEFSARLREIEQKIYEEAGEEFNLNSPKQLGVILFEKMGLPVIKKTKTGYSTAVDVL
                     EQLREQAPIVEDILTYRQIAKIQSTYVEGLLKVIQSDGKVHTRYVQTLTQTGRLSSVD
                     PNLQNIPIRLDEGRKIRQAFVPREKDWLIYSSDYSQIELRVLAHISNDEHLKAAFLEG
                     QDIHASTAMRVFGIEKAEDVTPNMRRQAKAVNFGIVYGISDYGLSQNLGISRKAAQQY
                     IDTYFEKYPGVKEYMERIVREAKDQGYVETLYHRRRYLPDINSRNYNIRSFAERTAIN
                     TPIQGSAADILKIAMIELDKRLKETGLQATMLLQVHDELVFEVPEKELESLDKLVKEV
                     MEQAVSLHVPLITDSSWGKTWYEAK"
     gene            4236..5072
                     /gene="mutM"
                     /locus_tag="U2K71_001819"
     CDS             4236..5072
                     /gene="mutM"
                     /locus_tag="U2K71_001819"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HEM7315898.1"
                     /translation="MPELPEVETVRKGLERLVVGKTIQKVQVLWPKIIEQPETPIFEA
                     SLVGETIQSIGRRGKFLIFHLDHCELISHLRMEGKYQFTKENTPIDKHTHVLFFFEDG
                     SQLRYNDVRKFGRMTIVEKGASATYRGIMKLGPEPLPDSFLLADFANGLKKSHKAIKP
                     LLLDQRLVTGLGNIYVDEALWEAKIHPEQPANTLRPKEVEQLRLSIIDVLDRAIEAGG
                     TTIRSYLNALGESGGFQVALHVYQQTGKPCIRCGTPIVKTKVAQRGTHYCPNCQRLKI
                     AK"
     gene            5036..5665
                     /locus_tag="U2K71_001820"
     CDS             5036..5665
                     /locus_tag="U2K71_001820"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HEM7315899.1"
                     /translation="MSELSKAKNRKVGFVLGLTGGIATGKSTAAKVFQSHGFPLIDGD
                     VIARETVEPGTLALRKIVSVFGQEVLQPDGQLDRGKLGMIVFPSKEKRQKLDQLLDPF
                     IRKAIKDQIASLSSNHPLVIVDIPLLYEGHYDHYMDAVAVVYTTPETQLQRLMKRNQL
                     TLEQAQQRISSQLPIEEKKQRADILFDNNGTKEKLVGQIENWLFANRFL"
     gene            5717..6049
                     /locus_tag="U2K71_001821"
     CDS             5717..6049
                     /locus_tag="U2K71_001821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HEM7315900.1"
                     /translation="MGSLKNQKDKVVGNAKEKIGEMLNKENLIDEGRTQSETAEEKEK
                     QYQARIDEIEDQQAEEEAKRQDLTQERKNPTPDRSKTPLPESDHIPDEQKIEEQRMKH
                     YTGIEEKL"
     gene            6361..6852
                     /gene="nrdR"
                     /locus_tag="U2K71_001822"
     CDS             6361..6852
                     /gene="nrdR"
                     /locus_tag="U2K71_001822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor NrdR"
                     /protein_id="HEM7315901.1"
                     /translation="MRCPRCQHNNSRVIDSRQADDGRAIRRRRECENCSFRFTTFERI
                     EAAPLLVIKKNGEREEFNRDKILRGLIRSAEKRPVAMEQMEQIVNHVENRVRSLGENE
                     VSTNLIGEYVMEDLVDLDEIAYIRFASVYRQFKDMSVFLKELQDIVDKAKENNKENNK
                     ENN"
     gene            6875..8248
                     /locus_tag="U2K71_001823"
     CDS             6875..8248
                     /locus_tag="U2K71_001823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEM7315902.1"
                     /translation="MENAWKELQPKNIYQGRKAAPLREDSKNALLYLYQPLIGGEALS
                     IYFTLLSEISQESGKGPEGMHADLLSSLGCGIPQFYDARKKLEGIGLLDVYFKEDSDL
                     GPRFIYELLEPLIPKAFFKDSVLAFLLLEKVGERRFQKLTQFFEPKRHTAEGYQKITK
                     KFLDVYRFNEAVYAANQENIESLNKSFEESESVALLEADSTFDWQFLNDWLAKKHIAS
                     LDDPIIKQVDMYHQLYGFDELTLGELISQSYDFSEQKVSEKELQKAILAMQRRPVKQT
                     KETVAVSENSNEPSSNEHLTNAALQLIKEAQSIPPMKYLEAIKSEKGGYVSKQENWLL
                     QDLVSQSGLSSSVINVLLNYVLVIKNNASLNASYVNTVANEWAQKKIATAEEAIQHIR
                     QISNQAKQSKQKKQTNYQTGKRNVRREKLPDWVNQPKDEKQISSEKKAEIDRRFKEYL
                     AKKEGDI"
     gene            8248..9174
                     /gene="dnaI"
                     /locus_tag="U2K71_001824"
     CDS             8248..9174
                     /gene="dnaI"
                     /locus_tag="U2K71_001824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primosomal protein DnaI"
                     /protein_id="HEM7315903.1"
                     /translation="MEDVGKELKRIISKRNYQEKYAEMIAEVLNDADVQAFLSAHQEE
                     LTQADIEKSYAKLYEFVQEKRKYQKNDPTMIAPGYEPKLTLNFHYVDVTYVPTEALLA
                     RQRQEEIRSRVKAMDMPKDIQSASFENFERTDGRGYASLEALDFVEKYTADPKGYHKG
                     LYLVGSFGIGKTYLLGATARELAIAGFTTTLVHFPTFAVEMKQAIGKDQVAEKLDAVK
                     RSPILMIDDIGADAMSSWIRDEVFGVILQYRMQEQLPTFFSSNFTMDELEQHLSVTQR
                     GDEEPLKAKRIMERIRYLTKEIEMTGRNRRNG"
     gene            9303..9743
                     /locus_tag="U2K71_001825"
     CDS             9303..9743
                     /locus_tag="U2K71_001825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEM7315904.1"
                     /translation="MRAIFSSISVVGLFFLLAGCGSADSNTGSVDSIDAGQTVKTSST
                     KTERQSIFVGTIESITQVEAEEETLQLKLVDVMAKEDPANMGTSFSNDGVILNATAEK
                     ISVDINELKIGDKVQFTLVEMPIMTMSIPPQVPGDSIIDVSLNK"
     gene            9887..10588
                     /locus_tag="U2K71_001826"
     CDS             9887..10588
                     /locus_tag="U2K71_001826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenine-N(1))-methyltransferase"
                     /protein_id="HEM7315905.1"
                     /translation="MNHLDLSQRLAAVGDFVPESARLADIGSDHAYLPVALILRGKIE
                     YAVAGEVVQGPYESARKQVKKDGLEDRITVRLANGLDAIQPQDGITAVTIAGMGGSLI
                     RDILEAGKLNQRLSGQERLILQPNIGERTLRSWLQENQYKIQSEKILEENKKIYEIIV
                     AEKEQKPVTYNEMELMFGPFLLEEKSEIFKSKWTRELQQRKAIREQLKQAANQEARQK
                     QIEQEIQMIEEVLKP"
     gene            10585..11706
                     /locus_tag="U2K71_001827"
     CDS             10585..11706
                     /locus_tag="U2K71_001827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Nif3-like dinuclear metal center hexameric
                     protein"
                     /protein_id="HEM7315906.1"
                     /translation="MTISGRTFIQKFESYCPQWLAEEGDPVGLHIGTLDKPIQRVMMT
                     LDVRPEVVEEAIKKKIDLLIAKHPPIFRPVKRLVTDQPQEKMYADLLKHDIAVYAAHT
                     NMDIIWDGLNDWFCELLGIEVESYLVKTHEIHYKKLAVYVPVDHAQKMREVLAAAGAG
                     TQGDYTGTSFTSIGHGRFTPEAGAQPAIGKVGKTEQVQEAKVEVILPETIEKQVIQAM
                     RSAHPYEEPAYDLFAIDEPVEMFGLGRVGELPQEISIEAFVEQVKEAFQLDGLRIVQP
                     KNAKSSVKRIAICGGSGEKFYPQAIAQRADVYITGDIYYHTAHDMQSAGLIAIDPGHY
                     IESLCKQRFIEKFESWKQEENWDINFFVSETNTNPFQFN"
     gene            11721..12953
                     /gene="pepT"
                     /locus_tag="U2K71_001828"
     CDS             11721..12953
                     /gene="pepT"
                     /locus_tag="U2K71_001828"
                     /EC_number="3.4.11.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase T"
                     /protein_id="HEM7315907.1"
                     /translation="MYENLLPRFLRYVKTETRSDATSKTTPSTQTQVAFAQELKKELE
                     ELGLADVFYNEDNGFVMGTLPSNVDHEVRSIGFISHMDTADFNAVGVNPQIIENYDGE
                     STIVLDAEERYTLNTKDFPNLKNYEGHTLITTDGSTLLGADDKSGIAEIMTAMEILIN
                     NPSIPHGTIKVAFGPDEEIGVGADKFDVEQFDVDFAYTVDGGPLGELQYETFSAAQAN
                     ITIQGKNVHPGTAKDTMINALQLAIDFHNQLPADEVPEKTEGYEGFFHLMALDGNPEE
                     ASMSYIIRDHDREKFEARKAQITHIQEELNQRFDQERIKVDMYDQYYNMKEIIEKDMS
                     IVELAKQAMIELGISPVIEPVRGGTDGSKISYMGIPTPNLFAGGENMHGRFEFVSLQV
                     MEKATDVIVKIAELNTKS"
     gene            13085..13993
                     /locus_tag="U2K71_001829"
     CDS             13085..13993
                     /locus_tag="U2K71_001829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegS/Rv2252/BmrU family lipid kinase"
                     /protein_id="HEM7315908.1"
                     /translation="MEKLLVFYNKSSGKDEGEKLARWFQDYVKEKQPELYVELAETGP
                     SIDDAELVKKAEAIEADTLVIIGGDGTIHHIVQAFQKTLSQYHVGLLPGGTVNNLARV
                     LEIPLKKEEAADVILEGNVRKIDYGQVNDEVIISTLTIGILADTAVWISQEEKQRYGS
                     WIFIKKFFKLLLKKKKYHLDLETDEEHWKGKSQLLTVTMSNSVGGFTNFDDSATLDDG
                     KFHVTIVPSLDIFRFIFYLPRIMRGKFYTIPGITYFTAKRMKIQAQEKSVGTRTDGDT
                     TDDLPVELSVVSKGLTVYSPKKEEKA"
     gene            14028..14300
                     /locus_tag="U2K71_001830"
     CDS             14028..14300
                     /locus_tag="U2K71_001830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1294 domain-containing protein"
                     /protein_id="HEM7315909.1"
                     /translation="MDVLLHNPLWFYLFIVNFYLFCLMGYDKHQARTSGWRVPENNLI
                     FVSLLGGGLGGLISRKVFHHKTRKKKFTIGFAAGVLVDLILIYFFH"
     gene            14311..15357
                     /locus_tag="U2K71_001831"
     CDS             14311..15357
                     /locus_tag="U2K71_001831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flippase-like domain-containing protein"
                     /protein_id="HEM7315910.1"
                     /translation="MSSKGTKTKIILNLLLLIVIFAIIYYLINQSFADIFAELLSTSV
                     VVLAAAVILGTVYQIAEGRSIKEIAEPFAPNFTTKDGFLTSSYIAFYRIVSFGTGTLI
                     SEIYFYNKKGMKLSQGAGVTALHMIMYKLAVITYALAGLVIQFSLFYSNGPSMIWFII
                     AGILLTIVIVAFLLAMSVSLNVQIFFVSLSNKLFKSERLRKWVDTCNQQIYSLRETVQ
                     TIIQDRTALLRIYLWNMLKLVFWYVLPYLILIENHPKIDFLLTISFISFAVVLSGVIP
                     TPAGIGSFEFVYLLLFRPVVGTVDAVSSLLLYRFSSFILPFLYGFVYVIAERRRVIKE
                     EIIEVKKENKENQG"
     gene            15608..18217
                     /gene="clpB"
                     /locus_tag="U2K71_001832"
     CDS             15608..18217
                     /gene="clpB"
                     /locus_tag="U2K71_001832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HEM7315911.1"
                     /translation="MNIEKMTTTLQEAIAEAQQIAVTRKHQDIDIAHVWKIFLQSNHF
                     GRNFYTDAGLDVESFEHEIDRLLDEYPVVSGGNVQYGQNLSQNFFRLLNEADQIRESF
                     GDEFLSTEVVILALMKLKNYPLTVYLNKNGLSEKELRKNIEEMRGGERVTSQNQEEQY
                     KALEKYGVDLVQQVKSGKMDPIIGRDEEIRDVIRILSRKTKNNPVLIGEPGVGKTAIV
                     EGLAQRIVRKDVPENLKDKTIFSLDMGALIAGAKFRGEFEERLKAVLKEVKKSDGRIL
                     LFIDEIHNIVGAGKTEGSMDAGNLLKPMLARGELHLIGATTLDEYRQYMEKDKALERR
                     FQKVLVKEPTVEDTISILRGLKERFEIHHGVNIHDNALVAAATLSDRYITDRFLPDKA
                     IDLIDEASATIRVEMNSMPTELDQVTRRLMQLEIEEAALKKESDDASKKRLKNLQEEL
                     AELREEANAMKMQWETEKEEVNSVSAKRAEIDKAKHELEDAENNYDLERAAVLRHGTI
                     PQLEKELKELEAKAKDSEIKMVQESVTENEIAQVVGRLTGIPVTKLVEGEREKLIKLN
                     ETLHKRVIGQDEAVDAVSDAVIRSRAGLQDPNRPLGSFLFLGPTGVGKTELAKALAEN
                     LFDSEDHMVRIDMSEYMEKHAVSRLVGAPPGYVGYEEGGQLTEAVRRNPYTIVLLDEI
                     EKAHPDVFNILLQVLDDGRLTDSKGRVVDFKNTVLIMTSNIGSQLLLEGVTADGTIPE
                     AVAEQVNTLLRGNFKPEFLNRIDDTILFTPLSLDNVKGIVDKMVAQLAQRLEHQEILL
                     TISDEAKTWIAENAYEPAYGARPLKRFITKEVETPLAKEIVAGHVMPKSKVTITLLDG
                     QLHFKTEELEEIV"
     gene            complement(18368..19054)
                     /locus_tag="U2K71_001833"
     CDS             complement(18368..19054)
                     /locus_tag="U2K71_001833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315912.1"
                     /translation="MEGLSVIKEIKKVQIALLAFGVFVVCYNFYEFITQKYSTSQGIT
                     FIVESLLGIALIFMPQVILKVFKLKIPAAIVLFYWFFLFISVFLGTGMHLISIISFWD
                     KILHAVSPMVLTALGYGLIGYLMKDAEISKTSPWLFLLFGFAFAGLCGVFWEFWEFLC
                     DQFLGMNLQRFAASDGTLFVGRAALMDTMGDLLTNTIGAALMGLFAWSQSKKDERYFE
                     SYKLEKVKNT"
     gene            complement(19032..19526)
                     /locus_tag="U2K71_001834"
     CDS             complement(19032..19526)
                     /locus_tag="U2K71_001834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deoxyribosyltransferase"
                     /protein_id="HEM7315913.1"
                     /translation="MKIYFAAPMFAKSDLLYNQQLVKEIRELSSELSIYLPQENEAIN
                     DKTAYADSRMIALADTEKVLASDLMIALLDGLTIDAGVASEIGVAYAKGIPVIGLYTD
                     TRQQGGTHPKKIAALQETAENQFHYLNLYTIGLIKLNGKVVSSEIELLSEVKRFLDGG
                     TFSD"
     gene            complement(19546..20766)
                     /locus_tag="U2K71_001835"
     CDS             complement(19546..20766)
                     /locus_tag="U2K71_001835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7315914.1"
                     /translation="MDQKDFRENLELKPVTQEHIDQFNELLSYVFQVTEADIEESGFE
                     NKRAFIRSKKPILEVSKVFGWFHENKLISQIAIYPCEVNIHGARYKMGGVTGVGTYPE
                     YANHGLMQDLIIVALDNMRKNKQWISYLYPYSIPYYRRKGWEIMSDKLSFKIRDTQLP
                     KQIPVSGMVERLPVDHPDVFSVYDKFAQQNHGALFRSDFHWEEYWRFENEDERTAAVY
                     YDSNHEPRGVLFYWVAEEIFHVKEMFYLDQEARNGLWNFISAHFSMIYWVHGDIYKNE
                     PLAFLIEDSQIKEQIEPYFMARIVDVKEFLQRFPFVGTADAFHFIIEDPVAPWNNGVF
                     ALTWDEQGQVRVLNEPIGKSVRLDIQTLTCLMMNYRRASYLARIERLETDEETLKSLE
                     RIIPNMEAYFSDYF"
     gene            20913..22748
                     /gene="lepA"
                     /locus_tag="U2K71_001836"
     CDS             20913..22748
                     /gene="lepA"
                     /locus_tag="U2K71_001836"
                     /EC_number="3.6.5.n1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor 4"
                     /protein_id="HEM7315915.1"
                     /translation="MDIKKMKERQEQIRNFSIIAHIDHGKSTLADRILEMTHTVTSRE
                     MQDQLLDSMDLERERGITIKLNAVELNYTAKNGETYIFHLIDTPGHVDFTYEVSRSLA
                     ACEGAVLVVDAAQGIEAQTLANVYLALDNDLEILPVINKIDLPAADPERVRQEIEDVI
                     GIDASEAVLASAKSGIGIEDILEQIVEYVPAPSGDLDAPLKALIFDSIYDSYRGVVLN
                     VRITDGMVKPGDKIKLMSNGKTFDVTEVGVFSPKAVARDFLMVGDVGYITASIKTVQD
                     TRVGDTVTLADNPAAEALPGYRKMNPMVYCGLYPIDTSRYNDLREALEKLQLNDAALQ
                     FEPETSQALGFGFRCGFLGLLHMDVVQERLEREFNLELITTAPSVIYHVNKTDGTTAT
                     VDNPADFPEPVTIQDVEEPFVKAQIMVPNDFVGAVMELSQRKRGEFITMDYLDDYRVN
                     VVYNIPLSEIVFDFFDKLKSSTKGYASLDYEMSGYQKSKLVKMDILLNGEKVDALSFI
                     VHRDFAYERGKAIVEKLKKLIPRQQFEVPIQAAIGQKIVARSDIKALRKNVLAKCYGG
                     DVSRKRKLLEKQKEGKKRMKQIGSVEVPQEAFMAVLKMDEDEPKK"
BASE COUNT         7646 a         4100 c         4887 g         6193 t
ORIGIN      
        1 aaaatcttgc caagccccct tttttgcaac ttatgataaa cggtgttcaa aagcttgcat
       61 ctgcggtaat aagcccaatc aaatcaaaaa catgagaagc tgttttttct tggctgttct
      121 tattatttga tgcaggacag ctttttcacg acctctgtaa actataggaa tctgttaaat
      181 caagggagca gaacttgcct tttacgctac ttttggtaca ataatgtatg attaaaaata
      241 aactggaggg tttctatctt gaacataggt aagccgagaa aactaggaaa aacaataaat
      301 gaaatcgagg aaggcgactc gttatcctta acagaatcta ttgaagataa ccagcttttg
      361 ctgtatttgg gattgacgaa tgatgcaaat cctttgtata tccaacatga ctttgcacaa
      421 aaaacagaat atcaacagcc aatcgttcca tcagtgatgc tgatggggat catcacaagt
      481 gcaatttcaa agcatcttcc tggaccagga tcacatgttg tcaatttttc tgtcaatttt
      541 attgaaccgg ttttccatta tgaaacactg acttttcagt ttgaggtcat aaaagtagat
      601 aagatgaagg atgtggtgac gatttctgta gaagcggaga atacgaacga agatcgtgtg
      661 ttggatgctg tcgttatggt ccaaccgcca caagttcctg aattggaaat agcaatcgaa
      721 agtgaaggtg aattgaatga ataatccaag tacatcaatg tcaggtatca atcgttttta
      781 tgcaaaagtt tatggatttt taggtatggg acttgccctt agtgcagtca cttcttactt
      841 ggctctttgg gtatttcctt tgcaattagc aacttttatc aataattttc cactaggctt
      901 tgtgggtctg tggatcgttg aactgatttt agtaatggtt ctaggcgtaa aagcacaaaa
      961 gaatccgagc ttagcaatcg gtggatttat tgcctactct cttttgaatg gcataacatt
     1021 agcggttact cttgcatact acgatattgg aacagtaacg caagctttct tgacagctac
     1081 tttgatgttc atggggatgt ctgttgtcgg agtaacaaca aaaagagatc ttagcggggt
     1141 tggaagagca ggacaggcag ctttaatcgg agtgatcatt gcactagtct tgaacatctt
     1201 cattttgaga agtagcgtag ttgagctgtt catctcccta ctgttagtcg tcatctttgc
     1261 agggattaca gcttatgata accaaagcat ccgtaatatc tatgtccaga caaacggaac
     1321 agcacatagt ggaattgctg tattcatggc attgaagctg tatttggact ttatcaactt
     1381 gttcttggcg tttctgagaa tctttggacg gaacaactaa aaaagaaaaa aattcatcag
     1441 ttggggctat accgaattat ttggtatggt cccgcttttt cttttcgcat tccgcaggat
     1501 tctttgttag aatgaataag aactttacag taaggagtta agaagatgac aaaaaataaa
     1561 ttattactcg ttgatgggaa cagtgtggct tttcgcgcat tctttgcgct tcacaattct
     1621 cttgaacgtt tcaaaaatag aaacggtctt catacaaatg caatctatgc attcaatacg
     1681 atgtttgaaa atgttatgca aaaagagcaa ccgactcatg ttctggttgc gttcgatgca
     1741 ggaaaaacga cttttcggac agaaatgtat gcggagtaca aaggtggaag atccaaaaca
     1801 cctggcgaat tcaaagaaca aatgccatat atacgtgatt tgttgacagg actaggggta
     1861 cagtattacg aattgcccaa ttatgaagca gatgatatta tcggtacttt ggcagaaaaa
     1921 gtagacaaag atcaatttga tgtcgtcgtg ctttcaggtg atcgggattt gactcaatta
     1981 gctagtaaag aagtaaaagt ggatatcact gtaaaaggtg tcagcgatat cgaaagctat
     2041 acaccagaac atgtagcgga aaaatatgat gggttgacgc caaaacagat tattgacatg
     2101 aagggactag ccggagatgc ctctgacaat atccccggtg tgactaaaat cggtgaaaaa
     2161 acagctatca agctattgaa acaatatggc tcagtcgaag gaatttatga acatatcgat
     2221 gaaatgaagc aaagcaaaat gaaagaaaat ctgataaatg acaaagagca ggctttctta
     2281 tcgaaaaaat tggcgaccat caatacttca gcgcctgtgg atgtctctat cgactcattg
     2341 aagtatgaag ggaaaaatct cgataaactc gttccgtttt ataaagaaat ggatttcaat
     2401 cagttccttt ctaaattgaa tatcgtagaa gaagaagtaa aaatggatga tattttgttt
     2461 gaagtcgttc atgaattcaa agaggaaatg tttactacgg atatggcgtt gtacgtggaa
     2521 atgatgggcg acaattatca tacagaggaa atcgtcggag tagcgtgggg gacagaaaag
     2581 aaaatttatg tcacgaacga tctatctgta ttcgaaaacc gagcgttcca ttcttggatc
     2641 actgatccga ctcggctgaa aaaagtatat gatgcgaaac gaacttatgt tgctttaaac
     2701 cgctatactg gaaaaacgaa aggaatcgat tttgatgtct tgctaggtgc ataccttctc
     2761 gatacgaatg aaaaaagcac agatatcgaa ggaatagctg ctcattacgg atacaacgat
     2821 atccaatcag atgaagctgt atacggcaaa ggggcgaaaa aaggtttacc agaagaagag
     2881 gaactctttt ttgctcattt agcgcgaaaa gtagctgcaa tcaatgcgtt gacaccaaaa
     2941 ttgagccaag aactggcaga aaaaaaccaa gaagatcttt tccggaaaat ggaactgcca
     3001 ttgtctatca ttttggcaga gatggaaata agcgggatca aagtagatgc gactcgctta
     3061 caagaaatga agggcgagtt ctctgctcgc ttaagagaga tcgaacaaaa aatctacgaa
     3121 gaagccggcg aagaattcaa tttgaattca ccaaagcaat taggagtgat cttatttgaa
     3181 aaaatgggat tacccgttat caaaaaaaca aaaactggat attccacagc cgtagatgta
     3241 ctggaacagc ttcgtgaaca agcaccaatc gtagaggata tcctcaccta tcgtcaaatt
     3301 gcaaaaattc aatctaccta tgtcgaggga ctgctgaaag tgatccaatc agatggaaaa
     3361 gtgcataccc gttatgtcca gacattgacc caaacaggac gattaagctc tgttgatcct
     3421 aatttgcaga acatcccgat tcgattagat gaagggagaa agatacgtca ggcatttgtt
     3481 cctagagaaa aagactggct gatttattct tctgactatt cacaaatcga gttacgcgta
     3541 cttgcgcata tctcaaatga tgaacatttg aaagctgcct tcctagaagg tcaagatatc
     3601 catgccagca cagcgatgcg tgtatttgga atcgaaaagg cagaggatgt cacaccaaat
     3661 atgcgtaggc aggcaaaagc agtgaatttt ggtatcgtgt atggcatctc tgattacgga
     3721 ctttcacaaa atctaggaat cagccgaaaa gcagcacagc aatacatcga tacgtacttt
     3781 gaaaaatacc caggtgtcaa agaatatatg gaaagaatcg tacgcgaagc aaaagaccaa
     3841 ggctacgtcg aaacgctgta tcaccgccgt cgatatctgc cagacatcaa ttcaagaaat
     3901 tacaatatcc gttcttttgc ggaacgaaca gctatcaata caccaatcca aggaagtgct
     3961 gctgatattt tgaaaatcgc gatgatcgaa ttggataaac gattaaaaga aacaggtctt
     4021 caagctacga tgctgttaca agttcatgat gaacttgtct tcgaggtacc ggaaaaagaa
     4081 cttgaatcat tagataaatt ggtgaaggaa gttatggaac aagctgtttc tcttcatgta
     4141 ccactgatca ctgacagcag ttggggaaaa acgtggtacg aagcaaaata aacattgttt
     4201 aagacagagc aatataatga aggtgaaaag aaaaaatgcc agaattacca gaagtagaaa
     4261 ccgttcgtaa aggattggaa cggttagtag taggaaaaac gattcaaaaa gttcaggtct
     4321 tgtggcccaa gatcatcgag caaccagaga cacccatctt tgaggcttct cttgttggcg
     4381 aaaccatcca atccattgga cgtagaggga agtttttaat ttttcatctc gatcattgtg
     4441 aactgatctc acacttgcga atggaaggaa aatatcaatt tacgaaagaa aatacaccaa
     4501 tcgataaaca tacgcatgta cttttctttt ttgaggatgg aagccaactt cgctacaacg
     4561 atgtacgaaa atttggtcgt atgacaatcg tcgaaaaagg agcaagtgca acttaccgag
     4621 gaatcatgaa attgggacct gagcctttgc cagatagctt cttattagca gatttcgcaa
     4681 atggactgaa aaagtcccac aaagcaatca agccattgct gctagaccaa cgtctagtga
     4741 ctggtctagg taatatttat gtagatgaag cattatggga agcgaagatc catccagaac
     4801 aaccggcgaa cacactgcgc cccaaagagg tagaacaatt acgtctatcg atcattgatg
     4861 ttttggatcg tgcaatagaa gccggaggta ccacgattcg cagttatttg aatgcattag
     4921 gagaatcagg cggatttcaa gtagcactgc atgtctatca gcaaactggc aaaccatgta
     4981 tacgttgtgg aacgccgatc gtgaaaacaa aagtggcaca aagggggacg cactattgtc
     5041 cgaattgtca aaggctaaaa atcgcaaagt aggatttgtt ttaggtttga cgggtggaat
     5101 agctaccggt aaaagtacgg cagccaaagt ctttcagtct catggatttc ctttgatcga
     5161 tggcgatgtg attgccagag aaactgttga gcctggaacg cttgctctga ggaaaattgt
     5221 atcagtgttt ggccaagagg tattgcagcc tgatgggcaa ctagatcgag ggaaacttgg
     5281 gatgattgtt tttccatcaa aggaaaaacg gcaaaaactt gatcagctat tagatccgtt
     5341 tatccgaaaa gcgataaaag atcaaatcgc ctctctaagt tccaatcatc ctttagtaat
     5401 tgtagatatc ccattgctct acgaaggtca ttatgaccac tatatggatg cagtagctgt
     5461 cgtttatact acgccagaaa ctcagcttca gcgtttgatg aaacgtaatc aactgacgct
     5521 agaacaagcg cagcagagaa ttagtagtca acttccaatc gaagaaaaaa agcagcgtgc
     5581 ggatattttg ttcgacaaca atggaacaaa agaaaaactt gtgggacaaa tcgaaaattg
     5641 gctttttgct aatcgctttc tttgagtatc tgtgtcaatt ttgctatcct gagtatatag
     5701 aaagtgaggg atagcgatgg gatcattaaa aaatcaaaag gataaagtcg taggtaacgc
     5761 aaaagaaaaa atcggcgaga tgctcaataa agaaaatcta atcgatgaag gtcgtacgca
     5821 atcagaaaca gcagaagaaa aagaaaagca gtaccaagca agaatcgatg aaatagaaga
     5881 ccagcaggca gaagaagaag cgaagcggca agatttgact caagaacgaa aaaatccgac
     5941 acctgatcgt agtaaaacac cgctcccaga aagtgaccac attcccgatg agcaaaaaat
     6001 cgaggaacag cgaatgaaac actatacagg aatcgaagag aagttataaa atagtaaaaa
     6061 atcgagatgt gacttgaagc agatgagaag gtgttcatct gcttcaagtt ttttgtgcta
     6121 gatgactaaa tcagcttttg tgtaagttat ttctcagtaa tcaaggtgat cagtattgga
     6181 aaattgaaat ttagtaagga caggggacac aattacgcat gaaagtagaa aaaattgttc
     6241 ttatatccgt gttttttggg taggacagtc gcgtttgtga tttgtttcgt gttataatac
     6301 aactagttgt aaagaaagac acatccttta tataaaatgc gatgcaagag gtgagacaaa
     6361 atgagatgtc ccagatgtca acataataat tcacgagtaa tcgacagccg tcaagcagat
     6421 gatggacgtg cgatccgtcg ccgtcgcgaa tgtgagaatt gttcgttccg attcactaca
     6481 tttgaacgaa ttgaagctgc tccgttatta gtaatcaaga aaaacgggga acgcgaagag
     6541 ttcaatcgcg ataaaatttt aagaggactc atccgttcag cagaaaaaag acctgtagct
     6601 atggaacaaa tggaacaaat cgtcaaccat gtcgaaaacc gcgtacgcag tctcggagaa
     6661 aacgaagtat cgacgaatct aattggtgaa tatgtgatgg aagatttagt tgatttagac
     6721 gaaatcgctt atattcgttt tgcaagtgtt tatcgccagt tcaaagatat gtctgttttc
     6781 ttaaaagagt tgcaagatat cgtggataaa gcaaaagaaa ataataaaga gaataataaa
     6841 gaaaacaatt aagaaacaaa aaggaggcat ttccttggaa aatgcttgga aagagcttca
     6901 accaaaaaat atttaccaag gaagaaaagc ggctccgctg agagaagaca gcaagaacgc
     6961 cttgctgtat ctttatcagc cactaatcgg cggagaagcc ttgagtatct atttcaccct
     7021 gttatcagaa atctctcaag aatctggcaa aggtcctgaa gggatgcatg cagatctgct
     7081 tagtagtcta ggctgcggta tcccgcaatt ttatgatgca agaaaaaagc tagaaggaat
     7141 cggcttactg gatgtttatt ttaaagaaga tagtgatctg ggacctcgtt ttatttatga
     7201 gctgttagaa ccactcattc ctaaagcatt tttcaaagac tctgttctgg cttttcttct
     7261 tctggaaaaa gttggtgaac gacgattcca gaagcttacc caattctttg aacctaaaag
     7321 gcatacagca gagggatatc aaaaaatcac aaaaaaattt ttagatgttt atcgcttcaa
     7381 tgaagcagtg tacgctgcta atcaagaaaa catcgaatcg ctgaataaat cttttgaaga
     7441 atctgaatca gtagcattac ttgaagcaga ttcgacattc gattggcagt ttttgaatga
     7501 ttggctggca aaaaaacata ttgcaagtct tgatgatcca atcatcaaac aagtcgacat
     7561 gtatcatcag ctttacggtt tcgatgaatt gacacttggc gaactgatca gtcaatctta
     7621 tgactttagt gaacaaaaag tatctgaaaa agaactgcaa aaagccattt tggcaatgca
     7681 gagacgtccg gtgaagcaaa caaaagaaac agttgcggtt tctgaaaaca gtaatgaacc
     7741 ctcgtcaaac gaacatttga caaacgcagc gcttcagctg ataaaagaag cacagagcat
     7801 tccaccgatg aaatatctgg aagcaatcaa gtcagaaaaa ggcggatatg tttctaaaca
     7861 agaaaactgg ttactgcaag atcttgtttc tcaatcaggt ctttccagta gcgtgatcaa
     7921 tgttttgctg aattatgtac tagtgatcaa gaataatgct agcttaaatg ccagttatgt
     7981 aaatacagtg gcaaatgaat gggcacaaaa gaaaatcgcc acagcagaag aagcgatcca
     8041 gcacattcgg caaataagta atcaagcaaa acaatcaaaa caaaagaagc aaacaaatta
     8101 tcaaactggc aagcgaaatg ttcgacggga aaagcttcct gattgggtca accagccaaa
     8161 agatgaaaaa cagatcagca gcgagaaaaa agcagagatt gaccgccgtt ttaaagaata
     8221 cctggcaaaa aaggagggtg atatctagtg gaagacgtag gcaaagaatt gaagaggatc
     8281 atcagcaaac gcaattatca agaaaagtat gctgaaatga tcgctgaagt cttaaatgat
     8341 gcggatgttc aagcttttct aagcgcacac caagaagaat tgacacaagc ggatattgaa
     8401 aaaagttacg ctaaattgta tgaattcgtg caagaaaagc gtaaatatca aaaaaacgat
     8461 ccaacgatga tcgctcccgg ctatgagccg aagctgacgt tgaattttca ttatgtggat
     8521 gtcacttatg taccaacgga agcattgtta gcacggcaaa gacaagaaga aatcagaagc
     8581 cgagtaaaag caatggatat gccaaaagat attcaatcgg cttcttttga aaactttgaa
     8641 cgtacagatg gacgaggata tgcgagctta gaagccctag attttgtaga gaaatatact
     8701 gctgatccaa aagggtatca taaaggactg tatctagtag gaagttttgg tattggaaaa
     8761 acatatttat taggagcaac agcgagagaa ttagcgattg ctggattcac cacgacgcta
     8821 gtccattttc cgacatttgc cgttgagatg aaacaggcaa tcggaaaaga tcaagtggca
     8881 gaaaagttag atgcagtgaa acgttcacct attttgatga tcgatgacat tggtgcagat
     8941 gcgatgagta gttggatacg agacgaagta ttcggtgtta tccttcaata tcggatgcag
     9001 gaacagttac caaccttctt ctcatcgaat tttacaatgg atgaattgga acagcatttg
     9061 tccgtcactc aacgtggaga tgaagagccg ctaaaagcga aacggataat ggaacgcata
     9121 cgttatttga caaaagaaat cgaaatgact ggtcgcaacc gacgcaatgg ttgaacagtt
     9181 gacaagtcca actgacattt gacctagtag taaaagcagg tcagcgaatc attatatgga
     9241 cagaaatgac cggagacaat caccgcaaca agtaatgcag aaacttaaac ggaggatttt
     9301 ttatgagggc tattttttct agtatatctg tagttggtct attttttctt ttagcaggct
     9361 gcggtagcgc agatagcaac acaggttctg ttgattcgat tgatgcgggt caaacagtaa
     9421 aaacaagcag tacgaaaacg gagagacaaa gcatatttgt cggaacgatt gaaagtatca
     9481 ctcaagtaga agcagaagaa gaaacgcttc agttgaaact cgtcgatgtc atggccaaag
     9541 aagatccagc gaatatggga acgtcattca gcaatgacgg agtgatctta aatgctactg
     9601 ctgaaaaaat aagcgtggac ataaatgaat tgaaaatcgg tgacaaagtc caatttacat
     9661 tagttgagat gccgatcatg acgatgtcta tcccaccgca agttcctgga gattcgatta
     9721 ttgatgtttc attgaacaaa taaccaataa cattttttca aaaataagaa aatgttgttt
     9781 tatagaacga aaaggtatat tttttaagga gatatgggaa tttttttatt cctagtctcc
     9841 ttattttatt tggaaaatgc tataattatc tagaaggagt gaagctatga accatttaga
     9901 tttatctcag cgcttagccg cagtgggtga ctttgtccct gaatccgctc gtttagcaga
     9961 cattggatca gatcacgctt acttacccgt tgcattgata ttaagaggaa aaatcgaata
    10021 tgcagttgct ggtgaagtcg ttcaaggtcc ttatgaatca gcacgtaagc aagtcaaaaa
    10081 agatggatta gaagaccgta tcaccgtacg ccttgcaaat ggtctagatg ctatccagcc
    10141 acaagatggt attacagctg tcacgattgc gggtatggga ggttcactga tccgagatat
    10201 cctagaagct ggcaagttga atcaacgctt aagcggacaa gaacgtttga tccttcagcc
    10261 aaacatcgga gagcgtacat tacgatcttg gctgcaagaa aatcagtata aaatccaatc
    10321 agaaaaaata cttgaagaaa ataaaaaaat atatgaaatc attgtagccg aaaaagaaca
    10381 aaaaccagtt acttacaatg aaatggagct gatgttcggg ccatttctgc ttgaagagaa
    10441 aagtgagatc ttcaaatcaa aatggacaag agagcttcaa caaagaaaag ctatccgtga
    10501 acagctcaaa caagcagcta atcaagaggc acgtcaaaaa caaatcgagc aggaaatcca
    10561 aatgattgag gaggttttaa agccatgaca atcagcggac gcactttcat ccaaaagttt
    10621 gaaagctatt gtcctcagtg gctagctgaa gaaggagacc cagtcggact gcatatcgga
    10681 acattggata agccaatcca acgagtaatg atgacattag acgttcgtcc agaagtggtc
    10741 gaagaagcaa tcaagaaaaa aatcgatttg ctgattgcta aacatcctcc aatctttcgt
    10801 cctgtaaaac ggctggttac tgatcagcca caagaaaaaa tgtatgcaga tttgttgaaa
    10861 catgatattg ccgtctatgc agcacacacg aacatggata ttatttggga tggactgaat
    10921 gactggtttt gtgaacttct cggtattgaa gtggagagtt atttagtaaa gactcatgag
    10981 attcattata aaaaactggc cgtttatgta cctgtggatc atgcacagaa aatgcgtgaa
    11041 gtattagctg cagctggtgc tggtacacaa ggtgattata ccggtacaag ttttacctcg
    11101 attggacacg gtcgatttac tccggaagct ggtgcacaac cagcgattgg gaaagtaggg
    11161 aagacagaac aagttcaaga ggcaaaagtt gaggttattt tgccggaaac tatcgaaaaa
    11221 caagtgattc aagccatgcg gtcagctcat ccttatgagg aacctgctta tgatcttttc
    11281 gctatagatg aaccagtcga aatgtttggg ttaggacgag taggtgaatt gccgcaagaa
    11341 atttctatag aggcatttgt agaacaagta aaagaagcgt tccaactaga tggactgaga
    11401 atagtccagc caaaaaatgc aaaatcctct gtgaagcgca ttgcgatttg cggtggaagc
    11461 ggtgaaaaat tttatccgca agcaatcgcc caaagagcag atgtctatat cacgggagat
    11521 atctattatc atacggctca tgatatgcaa agcgctgggt tgattgcaat tgatcccggt
    11581 cattatattg aatcgttatg caagcaacga tttatcgaga aatttgaatc ttggaaacaa
    11641 gaagaaaatt gggatattaa ctttttcgtt tctgaaacaa atacaaaccc atttcaattt
    11701 aattaaaagg agagaatact atgtacgaaa atcttttgcc acgtttttta cgatatgtca
    11761 aaacagaaac acgctccgat gcaacaagca aaacgactcc atcgactcag acacaagttg
    11821 cttttgcgca agaattgaaa aaagaattag aagagttagg cttagctgat gttttctata
    11881 atgaagataa cggattcgtt atgggaacat taccaagcaa tgttgatcat gaagtgcgtt
    11941 ccattggatt tatttcccac atggatacag cagattttaa tgcagtagga gtcaatccac
    12001 aaatcattga aaactatgat ggcgagtcta cgatcgtact agatgcggaa gaacgctata
    12061 cattgaatac aaaagatttt ccaaatttga aaaattatga aggccacaca ttgatcacaa
    12121 cagatggcag tacattactg ggagcagatg acaaatcagg gattgcggaa atcatgactg
    12181 caatggagat cttgatcaat aatccttcta tccctcacgg taccatcaaa gtggcttttg
    12241 gtcctgatga agaaatcggt gtcggagcgg ataaattcga tgttgagcaa ttcgatgtag
    12301 attttgctta tacagtcgat ggtggtcctt taggtgagtt gcagtatgaa acgtttagtg
    12361 ctgctcaagc aaatattacg atccaaggga aaaatgtaca tccagggacg gcaaaagata
    12421 caatgatcaa tgctctacag ttagcaatcg attttcataa tcagcttcca gccgatgaag
    12481 ttccagaaaa aacggaagga tacgaaggtt tcttccattt gatggcattg gatgggaatc
    12541 ctgaagaagc ttctatgagt tatatcattc gcgatcatga tcgtgaaaaa ttcgaagcac
    12601 gcaaagcaca aatcacacat atccaagaag agttgaatca gcgatttgat caagaacgaa
    12661 tcaaagtaga tatgtatgat caatattaca acatgaaaga aatcattgaa aaagatatga
    12721 gcatcgtgga attagcgaaa caagcaatga tcgagcttgg tatctctcca gtgatcgaac
    12781 cagttcgtgg aggaacagat ggttcaaaaa tctcttacat gggtattccg acgccaaatc
    12841 tatttgccgg aggagaaaat atgcacggac gattcgaatt tgtttcttta caagtaatgg
    12901 aaaaagcaac agacgtgatt gtaaaaattg cagaattgaa cacaaagagt taaaatcgaa
    12961 aaagaacagc ttatgacaag gcgttcgtcc caatgatgga atgtcatctg tctaaggcta
    13021 aagggcagga caatggaatt ggttgtcccg ccctcttttt tgaagagtaa ataggaggta
    13081 aagaatggaa aagctgctag ttttttataa caaaagttca gggaaagatg agggtgagaa
    13141 attagcacgc tggtttcaag attatgtaaa agaaaagcag ccagaattat atgtagaact
    13201 agctgaaact ggcccatcga ttgatgatgc ggaattagtc aagaaagcag aagccataga
    13261 agctgatact ttagtaatta taggtggaga cggcacgatt catcatatcg tccaagcgtt
    13321 ccaaaaaaca ttatctcaat accatgtcgg attacttcca ggtggaactg tcaacaacct
    13381 tgcacgtgtt cttgagattc ccctcaaaaa agaagaagct gctgacgtga tcctggaagg
    13441 caatgtacgg aaaatagact atggtcaagt caatgatgaa gtcattatct ctacactgac
    13501 aatcggaatc ttagcagata cagcagtttg gatcagtcaa gaagaaaaac agcgttacgg
    13561 ctcgtggatc tttatcaaga agttttttaa gttattgctg aagaagaaaa aatatcattt
    13621 ggatttagaa acagacgagg agcattggaa aggaaaatcc caactgttga cagtcacgat
    13681 gtctaactcc gtgggcggtt ttacaaattt tgacgactca gcgaccctag atgatggaaa
    13741 atttcatgta acgatcgtac cttccttaga tattttccga tttatttttt atttgcctag
    13801 aatcatgaga ggcaaattct atacgatccc cggcattact tatttcacag ccaaacgcat
    13861 gaaaatccaa gcgcaggaaa aatctgtggg cactcgaacg gatggagaca cgacagatga
    13921 tcttccagtt gaattatctg ttgtatcaaa aggattaaca gtttattcac caaagaaaga
    13981 agaaaaagct tagaaatagt agttaacaga aagttggaag aagagtaatg gatgtattgt
    14041 tacacaatcc tttgtggttt taccttttca ttgtgaattt ttacttattt tgtctgatgg
    14101 ggtatgataa acatcaggct agaactagcg gctggcgtgt tcctgagaat aatttgatat
    14161 ttgtcagcct gttaggcggt ggtttgggag gattgatctc tcgaaaagtt ttccatcata
    14221 aaactcggaa aaagaagttt actataggat tcgctgcagg tgtacttgtt gatttgattc
    14281 tcatttattt tttccattaa ggagcaggag atgtcgtcta aaggaacgaa aactaaaatc
    14341 attctgaatc tgcttttgct gattgtgatt tttgccatta tttattattt aatcaatcaa
    14401 tcatttgcag atatctttgc cgaattgctg tctaccagtg ttgttgtttt ggcagcggct
    14461 gttatattag ggacagttta ccaaatcgct gaagggcgct caatcaaaga aattgctgaa
    14521 cctttcgcgc cgaattttac aacgaaagat gggttcttga cctctagcta tattgcgttt
    14581 taccgaatcg tttcgtttgg tacaggaacg ctgatctcgg agatttattt ttataacaaa
    14641 aaaggaatga agctatccca gggagcaggg gtgacagcac tccatatgat tatgtacaaa
    14701 ctggcagtga tcacttatgc attagctggc ttggtcatcc aattctctct tttttattct
    14761 aatggtccat caatgatctg gtttatcatc gctggtatcc ttttgacgat cgtgattgtt
    14821 gcctttttat tggcgatgtc cgtcagcttg aatgttcaaa tattttttgt cagcttatcg
    14881 aataagttat ttaaaagtga gcgtttgcgg aaatgggtgg atacatgcaa tcagcagatc
    14941 tactctttaa gggaaacggt ccaaaccatt atccaagaca ggactgcgct attgcggatt
    15001 tatctatgga atatgttaaa acttgtattt tggtatgtac ttccttattt gattctgatt
    15061 gaaaaccatc ctaaaataga ttttctactg accatttcgt ttatcagctt tgcagtagtg
    15121 ctttcagggg ttattcctac gcctgctggt attggttcct ttgaatttgt ctatttgtta
    15181 ttattcagac cagttgttgg aacagttgac gcagtttctt cgcttttact gtatcgtttc
    15241 agttccttta tcttgccatt tctctatgga tttgtgtatg tgattgctga acggcgaaga
    15301 gtgatcaaag aagaaataat agaagtaaaa aaagagaata aagaaaatca agggtaggtc
    15361 ttttataaga tctacccttt ttatcaaatc aaagaaaaac gttatggata agataatttt
    15421 acgtttgttt ggctcttttt ttatatacat acaatcaatt ttgttttttt aagatttata
    15481 taagtcgaga aaaaaagtca aaaaactatg tgtatatttt gaccaaaatt gaccaaagcg
    15541 atataatagt ttttgtaaag atcaaagaaa taagtgatct tacctttaat taggagggta
    15601 tacctgtatg aatattgaga aaatgactac gacgcttcaa gaagcgattg ctgaagctca
    15661 acagattgcg gtcactcgaa aacatcaaga tatcgacatt gcccatgtat ggaaaatctt
    15721 tttacaatct aatcattttg gtcgaaattt ctatacggat gccggattgg atgtcgaatc
    15781 ttttgaacat gaaattgatc gtctactgga tgagtatcca gttgtttctg gtggaaacgt
    15841 ccaatatgga caaaacctca gccagaattt ctttcgatta ttgaatgaag cagaccaaat
    15901 acgtgaaagc ttcggcgacg agtttctttc aacggaagtc gttattttag cgttgatgaa
    15961 attaaaaaat tatccgttaa ctgtttattt gaataaaaac ggattatctg aaaaagaatt
    16021 gcgtaaaaac atcgaagaga tgagaggagg agaaagagtg acctcacaaa accaagaaga
    16081 acaatacaaa gcattagaaa aatacggcgt tgatttagta cagcaagtca aaagcggaaa
    16141 aatggatcca atcatcggtc gtgatgaaga aatccgtgat gtcatccgta ttctttcacg
    16201 aaaaacaaaa aataatcctg ttctgatagg agaacccggt gtagggaaaa cggcaatcgt
    16261 tgaagggctt gctcaacgta tcgtacgcaa agatgtgcct gaaaatttaa aagataaaac
    16321 gattttttca ttagatatgg gtgcattgat cgctggagca aaattccgtg gtgaatttga
    16381 agaaagacta aaagcagtct tgaaagaagt caaaaaaagt gatggacgta tcctcttgtt
    16441 catcgatgaa atccacaata tcgtaggggc aggaaaaaca gaaggaagta tggatgccgg
    16501 aaatctgttg aaaccaatgc ttgctcgcgg tgaattacat ttgatcggtg ccacaacttt
    16561 agatgagtac cgtcaatata tggaaaaaga caaagcgttg gaacgtcgct ttcaaaaggt
    16621 attagtcaaa gaaccaacag tggaagatac gatcagtatt ctgcgtggat taaaagaacg
    16681 ttttgagatc caccatggtg ttaacatcca tgacaacgca ttagtagcag cagctacttt
    16741 atctgaccgt tatattactg atcggttctt accggacaaa gcaatcgatt tgatcgatga
    16801 agccagtgca acgattcggg tagaaatgaa ctctatgcca acggaattag atcaagttac
    16861 tagacgtttg atgcagttgg aaatcgaaga agctgcactg aaaaaagaat cagatgatgc
    16921 aagtaaaaaa cgcttgaaaa atttgcaaga agaattagct gaacttcgtg aagaagcaaa
    16981 tgcgatgaag atgcagtggg aaacagaaaa agaagaagtc aattctgttt cagcaaaacg
    17041 tgctgaaatc gataaagcaa aacacgaatt agaagatgca gaaaataact atgatttaga
    17101 acgcgctgct gtattgcgtc atggtacgat acctcaatta gaaaaagaat tgaaggaatt
    17161 agaagctaaa gcaaaagaca gcgagattaa gatggttcaa gagtccgtta ccgaaaatga
    17221 gattgctcaa gtcgttggca gattaacggg tatccccgta acgaaacttg tagaaggtga
    17281 acgagaaaaa ctaattaaat tgaatgaaac acttcataaa cgagtgattg gtcaagatga
    17341 agcagttgat gcggtcagtg acgcggtaat tcgttcacgg gctggattgc aagacccaaa
    17401 tcgtcctcta ggttctttcc ttttcctagg accaactggt gtcggaaaaa ccgaattagc
    17461 gaaagcgtta gctgagaact tgtttgactc agaagatcat atggtacgga tcgatatgag
    17521 tgaatatatg gaaaaacatg ctgtgtctcg tctagtcgga gctcctccag gctatgtagg
    17581 ttacgaagaa ggtggtcaat taacggaagc tgtcagacga aatccttata caatcgtctt
    17641 gttggatgaa atcgaaaaag cgcatcccga tgtcttcaat atcttgttac aagtcctaga
    17701 tgacggacgc ttgactgatt caaaagggcg agtagtagat ttcaaaaata cggttctgat
    17761 catgaccagc aatatcggtt cacagttact gctggaaggc gtgactgctg atggaacgat
    17821 tccagaagca gtggctgaac aagtcaacac gctattacgt ggaaacttca aaccagaatt
    17881 cttgaaccga atcgacgaca cgattttatt tacaccattg agcctagaca acgtgaaagg
    17941 aatcgttgac aaaatggtgg cgcaattggc acaacgtctg gaacatcaag aaatcctgct
    18001 gacaatcagt gatgaagcca aaacgtggat tgctgaaaat gcttatgaac cagcatatgg
    18061 tgcaagacca ttgaaacgat ttatcactaa ggaagtagaa acaccactag ctaaagaaat
    18121 cgttgcagga catgttatgc ctaaatcaaa agtaacgatt actttacttg atggacaact
    18181 tcatttcaaa acagaagagc tagaagaaat cgtctaatct tccattgaga ctataaaaga
    18241 aaaaaggggt ctgacacaac tagtgtcaga cctccttttt tcaaataaac agtgttccag
    18301 aagtagtttc ttcgaaaaat aagccgaaat ttcttgaagc tgcccgcttc tgtcacaacc
    18361 tcttttatta ggtatttttc actttttcta atttataact ttcaaagtaa cgctcatctt
    18421 ttttactttg agaccatgca aacaatccca ttaaggcagc acctatcgta tttgtcagca
    18481 agtcgcccat cgtatccatc aatgctgctc gtccgacaaa cagtgtgcca tcagaagcag
    18541 caaaacgttg aagattcatg cctaagaact ggtcgcataa gaattcccag aactcccaaa
    18601 acacgccgca aagtccagca aacgcaaagc cgaataagag gaacaaccaa ggactggttt
    18661 ttgatatctc agcatctttc atcaagtaac caatcaatcc atagccaaga gctgtcagga
    18721 ccattggact caccgcatgt aagattttgt cccaaaaaga gatgatactg atcaaatgca
    18781 tccctgtgcc taaaaagaca gagatgaaca agaaaaacca gtaaaacaaa acgattgctg
    18841 ctggtatttt aagtttgaaa actttcaaaa tgacttgtgg catgaaaatc aatgcaatcc
    18901 ctaataaaga ttccacgata aatgtgattc cttgagaagt cgaatatttt tgtgtgataa
    18961 attcataaaa attatagcag acaacgaata caccaaaagc aagtaaagct atttgcacct
    19021 ttttgatttc tttaatcact gaaagtccct ccatctaaaa atctttttac ttctgaaagc
    19081 aactcaattt ctgaagaaac cacttttccg ttcaatttga tcaaacctat tgtatataag
    19141 ttcaggtagt gaaattgatt ttcagctgtt tcttgtagtg cagcgatttt tttcgggtgg
    19201 gttccgcctt gctgtcgagt atcagtatat aagccgatca ctgggatgcc tttcgcataa
    19261 gccacaccga tttccgatgc taccccggca tcgatcgtca gcccatctag taaagcaatc
    19321 attaaatcac tagccaacac tttttctgtg tcagcaagag caatcattcg actgtctgca
    19381 taagcagttt tgtcattgat tgcttcattt tcttgaggaa gatagatcga aagctcagaa
    19441 gagagctctc tgatttcttt aactaactgt tggttatata acaaatcact tttagcaaac
    19501 attggtgcgg caaaataaat tttcatctaa acatcatcct ttttatcaaa aataatcgct
    19561 aaagtaagct tccatgtttg gaatgatacg ctctagtgac ttcagtgttt cttcatctgt
    19621 ttctaaccgt tcgattcttg ccaaataaga agcacggcga taattcatca tcaagcaagt
    19681 caatgtttgg atatccagtc gaacggattt tcctataggt tcatttaaga cacggacttg
    19741 tccctgttca tcccatgtta aagcgaagac accattattc caaggagcga caggatcttc
    19801 aatgatgaaa tgaaaagcat cagctgttcc aacaaacgga aaccgttgaa ggaattcttt
    19861 tacatcaacg atccgagcca taaaatatgg ttcgatctgc tctttgatct gactatcttc
    19921 aatcaaaaat gctaaaggct catttttata aatatctcca tgtacccagt agatcatgga
    19981 aaaatgtgcg gaaataaagt tccataagcc atttcgagct tcttgatcaa gatagaacat
    20041 ttcttttacg tgaaatattt cttcagctac ccagtagaac agaacacctc ttggttcatg
    20101 gttgctatcg tagtagacag cggctgtgcg ctcatcttca ttttcgaaac gccaatactc
    20161 ttcccaatga aaatcactgc ggaataaagc accatggttt tgttgggcaa atttgtcata
    20221 aacggaaaat acgtctggat gatcaactgg taatcgctca accataccac ttactgggat
    20281 ttgtttaggt agctgtgtat ctctgatttt aaaggaaagt ttatcagaca tgatttccca
    20341 tcctttacgc cgatagtagg ggatactgta aggatacaaa taagagatcc attgcttatt
    20401 cttgcgcata ttatccaatg caacgataat tagatcttgc atcaatccat ggttagcata
    20461 ttctggataa gttccaactc cagtcacacc acccatttta taacgtgcac cgtgaatatt
    20521 gacttcacaa ggatagatag cgatttgaga gatcagttta ttttcgtgga accatccgaa
    20581 cactttcgat acttccaaaa tcggtttttt ggaacggata aaagctcgtt tattctcaaa
    20641 cccgctttcc tcgatgtctg cttcagtcac ttggaacaca taggaaagca attcattgaa
    20701 ttgatcgata tgttcttgag tgaccggttt taattcaaga ttttctctaa aatctttttg
    20761 atccattttt tcatctccat ttattagctt gataacagta tagcaaaatt ctacaaaaaa
    20821 aacttctata gctgactaat taaaaactct ttaaattact gcttttcgtt ggtataatgg
    20881 aaagtgctga ttatatgaaa agaggagacg ctatggatat aaagaaaatg aaagaacgcc
    20941 aagagcagat tcgtaatttt tcgatcatcg ctcatatcga ccatgggaaa tctacattgg
    21001 ccgatcgtat tttagaaatg acgcacactg tgacgtctcg cgaaatgcaa gatcagctgt
    21061 tagactcgat ggatttggaa cgtgaacgtg gaatcacgat taaattaaat gctgtagaac
    21121 tcaattacac agcgaaaaat ggagaaacat atatctttca cttgattgac accccagggc
    21181 acgtcgactt cacctatgaa gtctctcgaa gccttgctgc ctgtgagggt gccgttttag
    21241 tggtcgatgc agcacaagga atcgaagcac aaacattggc caatgtttat ttagcgttag
    21301 acaatgattt ggagatcctg ccagtcatca ataaaatcga cctacccgca gcagatcccg
    21361 aacgtgtccg tcaagaaatc gaagacgtaa tcggaatcga tgctagtgaa gctgtattag
    21421 ccagtgccaa atcagggatt gggattgaag atattctcga gcagatcgtg gaatatgttc
    21481 ctgcaccaag tggagactta gatgcaccgc taaaagcttt gatctttgac tctatttatg
    21541 atagttaccg cggtgttgtt ctaaatgtcc ggatcacgga cggtatggtc aaacccggag
    21601 acaaaatcaa actgatgagt aatggcaaaa catttgatgt tacagaggtc ggggtcttct
    21661 cgccaaaagc agttgctaga gactttttga tggtcggtga tgtcggttat atcacagcaa
    21721 gtatcaaaac tgtccaagat acacgagtag gggatacagt aacgttagca gacaatcctg
    21781 ccgctgaagc tttaccaggt taccgaaaaa tgaatccgat ggtttattgt ggtctttatc
    21841 caattgatac ttctcgttac aacgatttgc gggaagcttt agaaaaactg caattgaatg
    21901 atgctgcttt acaattcgaa ccagaaacct ctcaagcatt aggttttggg ttccgctgtg
    21961 gattcctcgg acttcttcat atggatgtcg tccaagaacg cttggaacgg gaattcaatc
    22021 tagaattgat cacgactgca ccatccgtta tttaccatgt taataaaacg gatggtacga
    22081 ctgctacagt agataacccg gcagattttc ctgaacctgt aacgattcag gatgtagaag
    22141 aaccattcgt caaagcacag atcatggtcc caaatgattt tgtcggtgca gtaatggaac
    22201 tttctcaacg aaaacgggga gaattcatta cgatggatta tttagatgat taccgagtaa
    22261 atgtcgtcta taatattccg ctttctgaaa ttgtctttga ctttttcgac aaattgaaat
    22321 caagcacgaa aggctatgcc tcattggatt atgaaatgtc tggttatcaa aaaagcaagt
    22381 tagtgaagat ggacatcttg ctgaatggag aaaaagtcga tgcattaagc tttatcgttc
    22441 accgagactt tgcctatgaa cgtggaaaag caatcgttga aaaactgaaa aaactaatcc
    22501 cacgccaaca atttgaggtg ccaatccaag cagctattgg acaaaaaatc gttgcccgtt
    22561 cagacatcaa agccttgcgt aaaaacgtct tggctaaatg ttatggtgga gatgtttctc
    22621 gtaaacgtaa actcttagag aaacaaaaag aagggaagaa gcggatgaaa caaatcggct
    22681 ccgtcgaagt tccgcaagaa gccttcatgg ccgttctgaa aatggacgag gacgaaccga
    22741 agaaatagca agggtttgtt tgaaaacagt gtaaaaaata aaccatgttt acatggattt
    22801 gccaccggtt tgccaccggt ggtttt
//
LOCUS       DASHHB010000041        22423 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.042, whole genome shotgun sequence.
ACCESSION   DASHHB010000041 DASHHB010000000
VERSION     DASHHB010000041.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22423)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22423)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22423
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.042"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            6..305
                     /locus_tag="U2K71_001837"
     CDS             6..305
                     /locus_tag="U2K71_001837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7315916.1"
                     /translation="MKNRLIVACGSGVATSQTIASKIQSMLEDDGIAFPVEAVDYKSI
                     QNELLTAGIYVYVAQPDEEVLEQAKDLGIEVFPGIPFLTGMGVEPIYDSIKELVQ"
     gene            378..1739
                     /locus_tag="U2K71_001838"
     CDS             378..1739
                     /locus_tag="U2K71_001838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS galactitol transporter subunit IIC"
                     /protein_id="HEM7315917.1"
                     /translation="MEVFQTIINYVLNLGSAIFVPLIILILGLLAGMKLKKAFMSALT
                     LGIAFSGMSMVIGYMSNAVSPASEALAKNTGISLPALDLGWTGAASITWSWSYAFIFF
                     AVTIGVNFVLLLFNWTKTLNVDMWNVWGKALTAYLVYYVSGSLAAGFLTAMVQVILEL
                     KLGDMFQKHIQDLTGIPLVTVTHFMTSAAVLLLPFNMIMDKIPALNKRADTNALKKKI
                     GIFSENSVMGFIIGLGLGFAAAYGVSGSLNLAIQTATALSLFPMISKMFMQSLSPLAD
                     AMSEFMKKRFKDREVYIGLDWPILAGRSEIWVTAILLVPVFIGYAIILPGNQVLPLAG
                     IINYSIAVGGLLLTGGNLARMLILGIITMPIYLYGATYLAPILSDLAEKSGAVEGIKK
                     GQLITWSSIEGPEFRVLFAEAFNRNILAIVGCVIFLGLFVLLYKYMIKAKVPSERYAE
                     LQK"
     gene            1761..2021
                     /locus_tag="U2K71_001839"
     CDS             1761..2021
                     /locus_tag="U2K71_001839"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315918.1"
                     /translation="MKREETDKIKWTVALCGTLLLFLYGLFTQNIIINLLVIFFALVI
                     YKYGNHVLFREYDEKRKRKIEESMKIKEATKEILREKSFIKR"
     gene            2035..2787
                     /locus_tag="U2K71_001840"
     CDS             2035..2787
                     /locus_tag="U2K71_001840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HEM7315919.1"
                     /translation="MSDGRMLFERERQDMARIVRVIFERKNTNVAGGNFSFKTTDDQG
                     KDYIIMTPTMMSEAYLGEISPAQVLVVEPRTRRIVDGVGHLTREINMHEAMYETNPAI
                     KAVLHAHAPNAMFWATTGLPMPNLTEATAKVKEIPVMDFEPNCSEELAELTSDYVKKQ
                     HLELPHMLLLNSHGVLINTGGESGIEAIHKALQILDTVEWNAEIAYKQTIFQALGITD
                     GFYSKGEKIGGLEELKHKTAIYNKSVIAAGGD"
     gene            complement(2881..3486)
                     /locus_tag="U2K71_001841"
     CDS             complement(2881..3486)
                     /locus_tag="U2K71_001841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM7315920.1"
                     /translation="MTYIFEKGTSGKHLILLHGTGGDEHSLLDIAHFLAPNSTLLSFR
                     GTVQEDGMNRFFKRNGLNQFDLDSLEKETDKLHEKIKEISEKELIPLSDWLLVGYSNG
                     ANIAAHLLLERETELNKGLFFHPMSLGVHQSRSTLEDKTVWISYGDNDPIVSPASFKE
                     LADQFETRQANVTIQLTVTGHQLTMDEIKQAREWTDHLLTK"
     gene            complement(3539..4513)
                     /locus_tag="U2K71_001842"
     CDS             complement(3539..4513)
                     /locus_tag="U2K71_001842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HEM7315921.1"
                     /translation="MDTQIRGIHHVTAMTSSATKIYRFFTDILGMRLIKKTVNQDDIE
                     TYHLYFTDDTGAPGTDMTFFDFPNQPKGKKGTDTISRASFRVPSDAALDFWLERFEKY
                     DVTHSAINERFGKKYIEFEDFDNQYYQLISDENNQGVAAGIPWKNSNVPTEFAITGLG
                     PVFVRVSQLEHIRLILEKILGFKETDHSGDFYLFEIGEGGNGASIIIEHRTDLPKAIE
                     GFGNIHHLALRVADKEALEYWIQVINKIELPNSGFVDRFYFQSEYFLAAHHVLFELAT
                     DGPGFLEDEPAESAGEKLSLPPFLEERREAIEAYVRPFDTSDANKKRE"
     gene            complement(4891..5610)
                     /locus_tag="U2K71_001843"
     CDS             complement(4891..5610)
                     /locus_tag="U2K71_001843"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM7315922.1"
                     /translation="MPKYEEIANILRERIRSKEYPLDSFLPNQVDLVKEFGASRMTIK
                     KAINILAMEGLVYSQRGSGTKILNHPFMQKDTSPANEYEGLSLQMKRRHRSLKSQIIH
                     FDVEFPNQYIQERLMIDAEQPVYNIHRLRILDGEPYVLEHTFMPVHLVPGLKKEHLLA
                     SIYDHLHQELNVHFAGAYRAISADKSDEYDQQYLNCAENDPVLEVQQVVYLKDGQPIE
                     YSSSRNRFDVRNYSVLDVRST"
     gene            complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U2K71_001844"
     CDS             complement(5737..7008)
                     /gene="celB"
                     /locus_tag="U2K71_001844"
                     /EC_number="2.7.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS cellobiose transporter subunit IIC"
                     /protein_id="HEM7315923.1"
                     /translation="MNGFMDKLSEKIMPLANLLGQNRYLTVLRDAFMLSFPLTMFGSI
                     VVVINNLPFFSDATKGTLSNLFGNGQNATMSIMSVFVTFGIGYYLSKSYDVEGIFGGA
                     VSFASFLILTPFAMKTAGGEDVTGVLSLDRLGAKGMFIGMIAAFLAGEIYCRITKRGW
                     QIKMPDGVPPAVTKSFAALIPAVVTLTIFLVINAVMTGIFNANLHDVVYEVIQKPLTG
                     LGSSLPATLIALFFVQFLWFFGLHGQIIVNSVMDPIWNTLMLDNLEAYQHGKELPHII
                     TKPFMETFTVGIGGSGMTLAVVLLMAFVLKKKQYRDVGRLALAPGIFNVNEPAIFGLP
                     IVLNATILIPWVVAPLIVTTFNYLVMAAGLVPAPTGVSVPWTVPIIASGVLATNSWLG
                     GMLQVIDFVIVAFIWYPFLKVLDKQPDLDIA"
     gene            7161..8558
                     /locus_tag="U2K71_001845"
     CDS             7161..8558
                     /locus_tag="U2K71_001845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 1 protein"
                     /protein_id="HEM7315924.1"
                     /translation="MKTYTFPENFWWGSAASGPQTEGRVSDDGKGENIWDHWYELEPE
                     KFFDQVGPEKTSQVYTKYKEDVQLMKETGHNTFRTSIQWSHLIPEGTGEINQKAVSFY
                     RDYFTELLDNGIEPFVNLYHFDMPLPLQEKGGWLSRETVDAYVSYAAACFELFGDLVK
                     KWFTHNEPIVPVEGGYLYQFHYPNHINMKEAVQVGYHENLASAKVIKAYHEGGHDGEI
                     GIILNLTPSYPRDETNPEDVKAAKIADAFFNRSFLDPAVKGEFPQELIEIVKELAIMP
                     EIHEGDLELIRENTIDLLGINYYQPRRIKAKETPINHEHSPMPDDYFDNYDMPNKKMN
                     PYRGWEIYEKGIYDILTNTRENYGNIKCYISENGMGVEGEERFVNSEGIIEDDYRIEF
                     VTDHLKYVHQAIQEGTNCVGYHMWTCMDNWSWTNAYKNRYGFIAVDLAQDGKRTIKKS
                     GRWFKTVSEKNGFDA"
     gene            8639..9079
                     /locus_tag="U2K71_001846"
     CDS             8639..9079
                     /locus_tag="U2K71_001846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM7315925.1"
                     /translation="MVDTYRNILVAIDGSEKAEKAFLEAITIAKRNQATLHILYVNEV
                     TGNYFGDFAFVTTSLQEELDEVAENQMKEHRNLAIEKGLTDIETYVLYGYPKTLIANF
                     HESKEPIDLIVMGATGLNAVERALVGSTTSYVVNHASCNVMVVK"
     gene            9227..9553
                     /locus_tag="U2K71_001847"
     CDS             9227..9553
                     /locus_tag="U2K71_001847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM7315926.1"
                     /translation="MISSDGIRGYNDAIILSILNKNDSYGYEISKTIRERTNDSYVIK
                     ETTLYSVFARLEKNQLIVSYPGEVTHGKKRTYYKITEIGRNLLEEKKQEWLTTKDVVD
                     QFLEGN"
     gene            9554..10303
                     /locus_tag="U2K71_001848"
     CDS             9554..10303
                     /locus_tag="U2K71_001848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315927.1"
                     /translation="MDTIRNYLDSLFIGVPQSTEIDKLKTDLLANMEDHYHELMGEGK
                     NEQEAIGTVISTFGSIDELLEELDVEKKHQADETETNTASIYLSEAENYWKEYRAASL
                     QVASGVLFISLSFASFLFFCSAGYVFMGISCLIFGIALAVGFFIASGMKITRLNHFLH
                     HRKIPEKVLAEAKEKEEEYQRSFGFSLIAGIGLCIFSLFPLLASLMWYMDGSIGASIF
                     FVTVGTGVFLIIYGSLVRHSYRQFTQSAYYW"
     gene            complement(10383..10838)
                     /locus_tag="U2K71_001849"
     CDS             complement(10383..10838)
                     /locus_tag="U2K71_001849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spx/MgsR family RNA polymerase-binding
                     regulatory protein"
                     /protein_id="HEM7315928.1"
                     /translation="MLTIYSSRTHSYHLMANWLKDHQMPYRERIFTPKQPLTRKEIFY
                     LLSLTENGFDDLLAKRSIDYKNLKDQIDQCTTSELVDLIVSHFTLLQRPIITDGKKLL
                     VGFNEDSMRLFIPVQWRRSQLKSVYNDFTWVSNLENSTDLDDEFLNYYI"
     gene            complement(11265..11540)
                     /locus_tag="U2K71_001850"
     CDS             complement(11265..11540)
                     /locus_tag="U2K71_001850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315929.1"
                     /translation="MKLEDKKVILKKIKRLPDQIDGYTICSFKKDRSIKAYTQNTEKG
                     IIYFLHEEGYDTQTFMFTEKKEFHKQMKKLIEKEFPRSHKLYVNQQF"
     gene            11834..12649
                     /gene="thiD"
                     /locus_tag="U2K71_001851"
     CDS             11834..12649
                     /gene="thiD"
                     /locus_tag="U2K71_001851"
                     /EC_number="2.7.1.49"
                     /EC_number="2.7.4.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional hydroxymethylpyrimidine
                     kinase/phosphomethylpyrimidine kinase"
                     /protein_id="HEM7315930.1"
                     /translation="MEDQYVQAMTIAGSDSDGSAGMQADMHTFFTRNVYGVSVMTACV
                     AGNSYGIGASVTLPTDFIDKEFELIAKDFQIRAAKTGMLADSKLIETVVKNYQKYDFG
                     PLVVDPVIVTKHGNLLLEESALQSLKEKLVPLAEVLTPNFYEAEKLTGRTLAAEEDFV
                     QAAKELQDMGAKNVMIKGHHAKNAQQETVNDYVLFESGKSFWLNAPFYPTDRINGTGD
                     SLSACIAAELAKGKTVEQAIRLAKDYVNRAIREEINVGHKYGPINHWAAGELE"
     gene            complement(12978..14429)
                     /gene="cls"
                     /locus_tag="U2K71_001852"
     CDS             complement(12978..14429)
                     /gene="cls"
                     /locus_tag="U2K71_001852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEM7315931.1"
                     /translation="MVSSIITALYLLNALIALVAILIKPRDVAAIWAWLLVLFALPGV
                     GFVLYLFFGRGLTDKKKFYLRQSDLKELENFQSFRGDTIEHYDPDMGDKDKQQFVDFF
                     SSLNRMPLTRMNSVTLLTDGQEKLDSLLQDLKKAKHSIHIEYYAFVTDNIGQQVLHVL
                     EEKAAEGVEVRILYDAFGSHGTKAKDFNRLIKNGGHVHTFVTSQRALLRFRLNYHDHR
                     KIVVIDGKISYTGGFNIANQYVNTTKKFGYWRDTHIRIFGAASSLLQLRFLTDWNVSV
                     PEEKKVGYHLNYFFKKADRDESKLADTSIQLVSSGPNNEREQIKLSFIKLITSAKKRV
                     WIQTPYLVPDESVIAALKIATASGVDVKIMIPNKPDHPFIYRATQYYARQLIKENVQI
                     LVYENGFLHAKTLIMDDEICMVGSANQDIRSYRLNFETSAVIYDPEFLEELATQFKED
                     ETHCSSMTTETVKEMSNWLLFKQQISRLFSPIL"
     gene            14770..17436
                     /locus_tag="U2K71_001853"
     CDS             14770..17436
                     /locus_tag="U2K71_001853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM7315932.1"
                     /translation="MENKEQAQQAVYKETIDSLMQQTQSSTDGLSTTQAKEKLERDGL
                     NQLKEKPKKPTWKLFLETFKDAMVIVLLIVALIQMVMGDYIEFLVIFAVLMLNSIISV
                     VQTKKAEGSLEALKNLSAPDARVIRDGKEKTIPANELVVGDIVLLDAGDYVPADGRLL
                     DAGSLKVDEGMLTGESTSAEKEVTDITSTVPIGDRTNMVFSGTIVTYGRGKFLVTSTG
                     NNTEIGQVAGLLESTTEQVTPLQRGLDKFSKKLSLAILALSLLILGIQLFRIYLGEGT
                     GDMTADIVSAVMFAVAVAVAAIPEALQSIVTIVLSLGTNKMAKRHAIIRKLPAVETLG
                     STSIICTDKTGTLTQNKMTVVDYFLGNGDTGDFTNDPSKWTADERRLIEISVLANDAS
                     ISEDGTKLGDPTEVAFIDFSEKLNQPYQEIRNNYPRQAELPFDSDRKLMSTGHTIDNK
                     NYLFVKGGPDIVFQRSKKVLLNGEVVPMTDELMKKFQEQNEAFSTRALRVLAFAYRPL
                     DNFDLSFEDEDDLILVGLLAMIDPPRAEVTQAVTEARSAGIKTIMITGDHKTTAVAIA
                     KQIGIFEEGNLALTGTELDELTEDQLNEVLEKVTVYARVSPENKIRIVRAWQNKGHIS
                     AMTGDGVNDAPALKQADIGIAMGTGTDVAKDAAAMVLTDDNFASIVSAVEVGRNVFDN
                     IKKAISYLFAGNLGAIIAIIFALVADWSNPFTALQLLFINLVNDSLPAIALGTEKAEP
                     ATMNRPPRDPNSGIFTDKTLISVTYRGVLIGIAVIFAQWLGMQHSPEFGVAMSFSTLI
                     WCRTLQTFPARSNTQTAIQAGFFSNRSVLLAVVVCSALYCLTMIPGLKEVFSIPSAFG
                     LTEVFTCMGIALVTIVLMEITKLIIVRVQGNK"
     gene            17800..18552
                     /locus_tag="U2K71_001854"
     CDS             17800..18552
                     /locus_tag="U2K71_001854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM7315933.1"
                     /translation="MNEKEWDEFAEEYYEIQQESQVTIAEDVKNYLKTEKVLPAETAA
                     DVAGGSGRFLSLAKEINRYDLIDFSSGMLENAKKEAEKNELPNVSFIKQSLEEFIQSN
                     ETYEMVFTAANPALVHLIQLEKLMKKSSKWCVIIRVVESIDDVFYPIEKQLGMIEEDP
                     NTSPVVMDQFETYLIDEGFKVKKKDFTYFTQESITEELMKLYYDAYAKDPVVTRYIRK
                     LFSEKREILSTTKLTYRLLLWSADKKRTFHDS"
     gene            complement(18574..19728)
                     /locus_tag="U2K71_001855"
     CDS             complement(18574..19728)
                     /locus_tag="U2K71_001855"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HEM7315934.1"
                     /translation="MKIGIDRLSFFIPNLYLDMTELAESRGDDPAKYHIGIGQDQMAV
                     NRANEDIITLGANAASKIVTEKDRELIDMVIVGTESGIDHSKASAVIIHHLLKIQSFA
                     RSFEVKEACYGGTAALHMAKEYVKNHPERKVLVIASDIARYSLASGGEVTQGVGAVAM
                     MITQNPRILSIEDDSVFLTEDIYDFWRPDYSEFPVVDGPLSNSTYIESFQKVWNRHKE
                     LSGRGLEDYQAIAFHIPYTKMGKKALQSVLDQTDEENQERLMARYEESIRYSRRIGNL
                     YTGSLYLGLTSLLENSKSLQPGDRIGLFSYGSGAVSEFFTGYLEENYQEYLFAQSHQE
                     MLDSRTRITVDEYETIFSETLPEHGECAEYTSDVPFSITKIENDIRYYKI"
     gene            19913..22354
                     /locus_tag="U2K71_001856"
     CDS             19913..22354
                     /locus_tag="U2K71_001856"
                     /EC_number="1.1.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA reductase,
                     degradative"
                     /protein_id="HEM7315935.1"
                     /translation="MKEVVMIDAARTPIGKYRGSLSPFTAVELGTLVTKGLLDKTKLK
                     KDKIDQVIFGNVLQAGNGQNVARQIALNSGLPVDVPAMTINEVCGSGMKAVILARQLI
                     QLGEAELVIAGGTESMSQAPMLKQYQSETNEYGEPISSMVNDGLTDAFSNAHMGLTAE
                     NVATQFSVSREEQDRYALSSQLKAAHAVEAGVFSEEIIPVKISDEDVLSEDEAVRGNS
                     TLEKLGTLRTVFSEEGTVTAGNASPLNDGASVVILASKEYAENNNLPYLATIKEVAEV
                     GIDPSIMGIAPIKAIQKLTDRSGMNLSTIDLFEINEAFAASSIVVSQELQLDEEKVNI
                     YGGAIALGHPIGASGARILTTLAYGLLREQKRYGIASLCIGGGLGLAVLLEANMEQTH
                     KDVQKKKFYQLTPSERRSQLIEKNVLTQETALIFQEQTLSEELSDHMIENQVSEVEIP
                     MGIAQNFQINGKKKWIPMATEEPSVIAAASNGAKICGNICAETPQRLMRGQIVLSGKS
                     EYQAVINAVNHRKEELILCANESYPSIVKRGGGVQDISTREFMGSFHAYLSIDFLVDV
                     KDAMGANMINSILESVANKLREWFPEEEILFSILSNFATESLASACCEIPFERLGRNK
                     EIGEQIAKKIQQAGEYAKLDPYRAATHNKGIMNGIEAVVAATGNDTRAVSASIHAYAA
                     RNGLYQGLTDWQIKGDKLVGKLTVPLAVATVGGASNILPKAKASLAMLDIDSAKELAQ
                     VIAAVGLAQNLAALRALVTEGIQKGHMGLQARSLAISIGAIGEEIEQVAKKLREAEKM
                     NQQTAIQILEKIREK"
BASE COUNT         7198 a         3933 c         4673 g         6619 t
ORIGIN      
        1 aaaaaatgaa aaatcggtta attgtcgcat gtggaagtgg agtagcaacg agtcagacaa
       61 tcgctagtaa gatccaaagt atgttagaag atgacggcat cgcatttcct gtagaagcag
      121 ttgattacaa atcgattcaa aatgagcttc taactgctgg aatctatgtg tatgtcgcac
      181 aacctgatga ggaagtactt gaacaagcaa aagatttggg tattgaagta tttccaggaa
      241 ttccattttt aactggaatg ggtgtagaac ctatttatga cagtatcaaa gagttagtac
      301 agtaaaggag gattgttgga taaaaaagtt ggttcatagt tttatttact atatttagaa
      361 agaaagaagg tttatttatg gaagttttcc aaaccattat caattatgtt ttgaacttag
      421 gatcagccat ttttgttccc ctgattattt tgatacttgg attgttggca gggatgaaat
      481 taaaaaaagc attcatgtct gctttgactt tagggattgc ttttagcgga atgtctatgg
      541 tcattggcta tatgtctaac gcagtaagtc cagcatcaga agcattggct aaaaatacgg
      601 gaatcagttt gcctgcactt gatcttgggt ggacaggtgc agcaagcatc acatggtcgt
      661 ggagttatgc ttttatattt tttgcagtaa cgattggggt caattttgta ctgttacttt
      721 ttaattggac gaagacgtta aatgttgata tgtggaatgt ttgggggaaa gctttaactg
      781 cttatttagt ttattatgtt tccggtagtt tggctgcagg ctttcttact gcaatggtcc
      841 aagttatatt ggaattaaag cttggtgata tgtttcaaaa acatatacaa gatctcacag
      901 ggattccatt agtaacagtg actcatttta tgacatctgc cgctgtcttg ttgctgcctt
      961 tcaatatgat catggataaa attcctgctt taaataaacg agcggatacg aatgcattga
     1021 agaagaaaat tgggatcttc tctgaaaact ctgtaatggg attcattatt ggattggggc
     1081 ttggctttgc tgctgcgtat ggcgtttcag gttctcttaa tctagcgatc caaacagcta
     1141 cagcattaag tttgtttcct atgatctcta aaatgttcat gcaatctttg tctcctctag
     1201 cagacgctat gtcagaattt atgaaaaagc ggtttaagga tcgtgaagta tatatcggat
     1261 tggactggcc gatattagct ggtcgttcag aaatttgggt tactgctatt ttacttgttc
     1321 ctgtgtttat tggttatgcc attattttgc caggaaacca agtattgcca ttagcaggga
     1381 ttatcaacta ttcgattgcg gtcggcggat tacttttaac aggaggaaac ttggctcgta
     1441 tgctgatctt gggaatcatc actatgccta tttatttgta cggggccact taccttgcgc
     1501 caatactcag tgatttagca gaaaaaagcg gagcagtcga gggaatcaag aaaggacagt
     1561 tgatcacatg gtcatccata gaagggccgg agttcagggt tttatttgcg gaggcattta
     1621 ataggaatat tctcgctatt gtaggatgcg tgatttttct aggactgttt gttctacttt
     1681 ataaatatat gataaaagcg aaagtaccga gtgaacgtta tgctgaacta caaaaatagc
     1741 attgaaatgg tgggaaagta atgaaaagag aagagacaga taaaataaaa tggacagtcg
     1801 ctctttgtgg tacacttctg ctatttctat atggactctt tactcagaat atcatcatca
     1861 atctattggt catttttttc gcactagtga tctataaata tggaaatcac gttttattca
     1921 gagagtacga tgaaaaaaga aaacggaaaa tagaagaatc aatgaaaata aaagaagcta
     1981 ccaaagaaat attgagagaa aaaagtttta taaaaagata atggaggggt cattatgtca
     2041 gatggacgta tgttatttga gagagaacga caggacatgg cacgtatcgt aagagtgatt
     2101 ttcgaaagga aaaacacaaa tgtcgcagga gggaattttt cctttaaaac cactgatgat
     2161 caagggaaag actacatcat catgacgccg accatgatgt cagaagccta tctaggagag
     2221 atttctcctg cacaggttct agtagtagaa ccaagaacgc gtagaatcgt tgacggagtc
     2281 ggtcatttaa ctcgtgaaat caatatgcat gaagcgatgt atgagactaa tccagctatc
     2341 aaagctgtcc tacatgctca tgcgcctaat gcaatgttct gggcgactac tgggttgcct
     2401 atgcctaatt tgacagaagc cacagctaaa gtaaaagaga ttcctgtcat ggactttgag
     2461 cctaattgct ctgaagaact tgctgaattg acaagcgact atgtaaaaaa acagcatctc
     2521 gaattaccgc atatgttgct gctcaactct catggcgtat tgatcaacac aggtggagaa
     2581 tcaggaatcg aagctatcca taaagcactg caaatccttg atacagttga atggaatgca
     2641 gaaattgcct acaagcagac gatttttcaa gcattaggga ttactgacgg tttttattca
     2701 aaaggtgaga aaatcggggg attggaagag ttgaaacata aaacggctat ttataataaa
     2761 tctgttattg cagctggtgg agactaacct atctgacaca aaaaactacg actttcacaa
     2821 aatctgatcc agatcgatat ctgcttcagg ttgtattatg taagtcgtag ttcttttctt
     2881 ttatttagtt aagaggtggt ctgtccattc cctcgcttgc ttgatttcgt ccatcgtcaa
     2941 ctgatgacct gttacagtta gctgaatagt cacatttgct tggcgtgttt cgaattgatc
     3001 ggccaattct ttaaaggaag caggggaaac gattggatcg ttatcaccat aagaaatcca
     3061 gacggtttta tcctctaatg tggaacggct ttgatgaaca cctagagaca tcggatggaa
     3121 aaacaaccct ttatttagct cggtctcacg ctccaataat aaatgagcag cgatgtttgc
     3181 gccatttgaa tagccgacaa gcagccagtc acttaacggt atcagttctt tttcgctgat
     3241 ttctttgatt ttttcatgga gtttatctgt ttctttttcc aaactgtcca ggtcgaattg
     3301 attcaatcca ttacgcttga aaaaacgatt catcccatct tcttggaccg tgccacgaaa
     3361 agaaagcaat gtactgtttg gtgcaagaaa gtgagcgata tccagtaaag aatgttcatc
     3421 gccacctgtg ccatgaagta agatcaaatg cttgccactt gttccttttt caaaaatgta
     3481 ggtcattttg ttccctcttt tctgtttcta tagatgcaaa tgaagtagca tgaagagatt
     3541 attcacgttt tttatttgca tcacttgtat cgaaaggacg gacataagct tcaatggctt
     3601 cacgacgttc ttctaagaat ggaggaagag atagtttttc tcctgctgat tctgctggtt
     3661 catcttcaag gaatccagga ccatcagttg ctaattcaaa cagtacgtga tgagcggcta
     3721 ggaagtactc agattggaaa tagaaacgat cgacgaaacc agaatttggc aattcaatct
     3781 tattgattac ttgaatccag tattccaatg cttctttgtc tgctacacgc aatgctaagt
     3841 gatggatatt tccaaatcct tcgattgctt ttggcaagtc tgtccgatgt tcaataatga
     3901 tgcttgcacc atttccccct tcaccgattt caaataagta gaaatctcct gaatggtctg
     3961 tttctttgaa tcctaagatt ttttcaagaa tcaagcggat atgctctaat tgactgacac
     4021 ggacaaagac aggtccaagt cctgtgatcg caaactctgt cggtacattg ctgtttttcc
     4081 aaggaatgcc agctgctaca ccttgattgt tttcgtcaga gatcaattga taatactgat
     4141 tatcgaaatc ttcaaattcg atatattttt tgccgaaacg ttcattgatt gctgaatgag
     4201 taacgtcata tttttcaaaa cgttccaacc aaaagtctaa tgctgcgtca cttggcacac
     4261 ggaaagaagc acgagagatc gtatctgttc ctttttttcc ttttggctga tttggaaaat
     4321 caaagaaagt catatctgtt cctggcgcac cagtatcgtc tgtaaagtaa agatggtaag
     4381 tttcgatgtc atcttggtta actgtttttt tgattaagcg cattcctaaa atatctgtaa
     4441 aaaagcggta gatttttgta gcgctagatg tcattgctgt gacgtggtga attcctctga
     4501 tttgtgtatc catgataaat ttcctcctgt taacttacaa aaagtaagta gttgatttag
     4561 acatagtata gagggtgcat ctctattttg caattaattt gtcttctaaa gtatcaggaa
     4621 ggaatcaaga atggaaaaga ggagggacgg atcaataaaa taaaaagact tacgactagc
     4681 tgtatcgatt tcaaaaaaac tagctttaaa aagctaactt tagaatcatt acgggtggtc
     4741 ataagtcttt ttatgctgag ttattttttc atttttgagc taatgcttag gatagaatag
     4801 aatgttgatt tcacgatctc ttcttgttac ttgaaactgc ttaccactgt cacgatctct
     4861 tggtaaacgg ctttttcaca acctctttgg ttaagttgaa cgcacatcca gcacagaata
     4921 gttccgaaca tcaaatcggt ttcggctgga agaatattca atcggctgtc catctttgag
     4981 atagacgact tgctgaactt ccagtacagg atcgttttct gcacagttca aatattgctg
     5041 atcatattca tcacttttat ctgcagaaat agcacgataa gcccctgcaa aatggacatt
     5101 caattcttga tgaagatgat catagataga ggctaacaaa tgttcttttt ttaaacccgg
     5161 cacaagatgg actggcataa aggtatgctc taaaacatat ggctcgccat ctaagatcct
     5221 taaccggtga atattgtaga caggttgctc ggcatcgatc atcaatcgtt cttggatata
     5281 ttgattaggg aattcaacat caaaatgaat gatttgactc ttcaaagaac gatgtctgcg
     5341 tttcatttgg agactcaaac cttcatactc gttagcgggc gatgtgtctt tttgcataaa
     5401 tggatgattc aagattttcg ttcctgatcc tcgctgcgaa tagacaagtc cttccattgc
     5461 taagatattg atggcttttt tgattgtcat ccggctcgca ccaaattctt ttaccaagtc
     5521 gacttgatta ggcaaaaaac tatcgagagg atactctttt gaacggatac gttctcttaa
     5581 aatatttgcg atttcttcgt attttggcac gttattcacc tcttctagag tataacgaaa
     5641 gattatgctt ccgacaactc gaagtagcat ctggaacatt gtttattcga aaaaagaggt
     5701 catgacaagt ttttgtcaca accccttaat gatctattaa gcaatatcta aatctggttg
     5761 tttatctagt actttcagga atgggtacca gataaaggca acgatgacaa agtcgatgac
     5821 ttgcagcata ccaccaagcc atgaatttgt tgctaagaca ccgcttgcaa tgattggcac
     5881 agtccatgga acagagacgc cagttggtgc agggacaagt ccagcagcca ttaccaaata
     5941 attgaacgtc gttacgatta gaggtgccac aacccaagga ataaggattg ttgcattcaa
     6001 tacgatcggt agaccgaaga tcgctggttc atttacattg aaaataccag gtgctaaagc
     6061 taatcgtcca acatcacgat actgtttctt tttaagaaca aaggccatca ataaaacgac
     6121 agctaaagtc attccagagc caccaatacc aacagtgaag gtttccatga atggttttgt
     6181 gatgatgtgt ggtaattctt ttccatgttg ataagcttct aagttatcaa gcatcagtgt
     6241 attccaaatc gggtccatta cagagttgac gatgatttgt ccgtgaagtc cgaagaacca
     6301 taagaattgg acaaagaaca aagcaatcaa tgttgcaggt aaactacttc ccaaaccagt
     6361 taatggtttt tggatcactt cataaacgac gtcatgtaag ttagcattga aaataccagt
     6421 catcacagca ttgattacta agaaaatagt tagtgtcact acagcaggga tcaaagcggc
     6481 aaatgatttt gttactgcag gcggaactcc atcaggcatt ttgatctgcc agccgcgttt
     6541 cgtgattctg cagtagattt ctccagccaa gaaagcagcg atcattccaa tgaacattcc
     6601 ttttgcgcca agacgatcaa gtgaaagcac accagtcaca tcttcaccgc cagcggtttt
     6661 cattgcaaaa ggcgtaagaa tcaagaagct ggcaaaagac acggcaccac cgaaaatacc
     6721 ttcaacgtca taagattttg ataaataata gccgatacca aaagtaacaa aaaccgacat
     6781 gatgctcatt gttgcatttt gcccattacc gaataaattc gataaagttc cctttgttgc
     6841 gtcactgaaa aaaggcaagt tattgataac gacaacgatc gaaccgaaca tcgttaaagg
     6901 gaaggaaagc atgaaagcat cccgcagcac tgtaaggtaa cgattttgcc ctaataaatt
     6961 agctaagggc atgattttct cagaaagctt gtccataaaa ccgttcattt ttattcacct
     7021 caaatttatt tgcaatcgat tacatacaaa gtataacgcc aaaaatttta aatgtctagt
     7081 cattttcata aaaagtattg atttattttt tagggttcgt tatgattacg gtatcaagaa
     7141 aaaagagagg aagtaaaaaa atgaaaacct acacattccc agaaaatttt tggtggggtt
     7201 ctgctgctag cggaccgcaa acagaaggaa gagtaagtga tgatggtaaa ggagaaaata
     7261 tttgggatca ttggtatgaa ttagagcctg aaaaattctt cgatcaagta ggacctgaaa
     7321 aaacttcgca agtttacaca aaatacaaag aagatgttca gttaatgaaa gaaactggcc
     7381 ataacacatt tcgtacatct atccaatgga gtcatttgat tccagaaggg acaggtgaaa
     7441 tcaatcaaaa agctgtttct ttttatcgag attattttac tgaattgctg gataatggta
     7501 ttgaaccttt tgtgaacctt taccatttcg atatgccact accactgcaa gaaaaaggcg
     7561 gatggttatc aagagaaacg gtcgacgctt atgtttctta tgcagcagcg tgctttgaac
     7621 tgtttggaga tctggtcaaa aaatggttta ctcataatga accaatcgta cctgtagaag
     7681 gaggctactt gtatcaattc cactatccaa atcatatcaa tatgaaagaa gcggtccaag
     7741 taggctatca tgagaatcta gcaagcgcaa aggtaatcaa agcctaccat gaaggcggac
     7801 atgatggtga gatcggaatc atcttgaatc tgacaccaag ctatccgaga gatgaaacga
     7861 acccagaaga tgtaaaagcg gcgaagatcg ctgacgcatt cttcaaccga tcattccttg
     7921 accctgcagt gaaaggagag tttccacaag agttgatcga aattgtcaaa gaattggcta
     7981 taatgccaga gatccatgaa ggagacctag aactcatccg tgaaaacacg attgacctgc
     8041 taggaatcaa ttactatcag cctcgtcgaa tcaaagcaaa agaaactcca atcaatcacg
     8101 aacatagccc catgccagat gattattttg ataattacga catgcccaat aaaaaaatga
     8161 atccatatcg tggatgggaa atttacgaaa aaggaatcta tgatatttta acgaatacaa
     8221 gagaaaacta tggtaatatc aaatgctata tttctgaaaa tgggatgggg gtcgaaggag
     8281 aagaacgatt tgttaacagc gaaggaatta ttgaagatga ttaccggatc gagtttgtta
     8341 cggatcattt gaaatacgtc catcaagcaa tccaagaagg aaccaattgt gtgggttacc
     8401 acatgtggac atgtatggac aactggtctt ggacaaatgc ttataagaat cgctatggct
     8461 ttatcgctgt tgatttggca caagacggta aacggacgat caaaaaatca ggtcgctggt
     8521 ttaaaacggt ctctgaaaaa aacggatttg atgcctgata ttttttgata aaacgttcac
     8581 aaaatgtttg ttctaacact gtaatcgtgc tacactaaca gtgaaggagg cgtttacaat
     8641 ggtagatact tatcgtaata ttttagtagc aattgatggt tctgaaaagg cagaaaaagc
     8701 atttcttgag gcaatcacga ttgccaaacg aaatcaggca acgttgcaca tcctgtatgt
     8761 caatgaagta acaggcaatt actttggtga ttttgcattt gttacaacaa gccttcagga
     8821 agaattggat gaggttgcgg aaaaccagat gaaagaacat cgaaacttgg caattgaaaa
     8881 aggcctgaca gatatcgaga catatgttct ttacggttat ccaaagacac tgattgctaa
     8941 tttccatgaa tcgaaagaac caatcgatct gattgtcatg ggggcgacag gtctgaatgc
     9001 ggtagaacgt gcactagtag gttccactac ttcctatgta gtcaaccacg catcatgtaa
     9061 cgtcatggtt gtaaaataaa taaattaagg gaacagaaag caaaaaagca taacgcttct
     9121 tttgctttct gttcctttta tttttttgta aaaaattact ttacctgatg aagtaatcat
     9181 gctattctaa ttacaacgac agatggagta atatggagga aagaaaatga tttcaagtga
     9241 tggaatacgc ggatacaatg atgcgattat tttatcgatc cttaacaaaa acgattctta
     9301 tggatatgaa atttctaaaa cgattcgtga gaggacaaat gattcctatg tgatcaaaga
     9361 gacaacactt tactccgttt ttgcccgttt agaaaagaat caactgattg tttcgtatcc
     9421 tggagaagtg actcatggaa aaaagcggac atattataaa atcactgaaa tagggcgtaa
     9481 tcttttagaa gaaaaaaagc aagagtggct gacgacgaaa gacgtagtcg atcaattttt
     9541 ggagggaaat taaatggata caatccgtaa ttatttagac agtcttttta tcggagtacc
     9601 tcaaagtacc gagatagata agctgaaaac tgatttgttg gcaaatatgg aagaccatta
     9661 tcatgaattg atgggagaag gaaaaaatga acaggaggcc atcggtacag taatttcgac
     9721 atttggttca atcgatgaat tattagaaga actagatgtc gaaaaaaaac atcaagctga
     9781 cgagacggaa acaaacacag cttctattta tttaagcgaa gcagaaaact actggaaaga
     9841 atacagagcg gcaagtctcc aagtggcatc aggtgttcta ttcattagct tatcttttgc
     9901 aagttttctt ttcttttgtt cagcaggata tgtgttcatg ggtatttctt gtctgatttt
     9961 cggtatagca ttagctgtcg gtttttttat tgcttctggt atgaaaatca cacgtttaaa
    10021 tcactttttg caccatcgga aaatacctga aaaagtatta gcagaagcaa aagaaaaaga
    10081 agaagagtat cagcgaagtt ttggcttctc actgattgca ggaattggat tatgtatttt
    10141 ttcacttttc ccacttttgg cttctttaat gtggtatatg gatggtagta tcggtgcctc
    10201 tatatttttt gttacggtag gaacgggtgt cttcttgatc atttatggca gtctagtccg
    10261 tcattcttat cgtcaattca ctcaaagcgc ttattactgg tgaatgtatt cagatttgta
    10321 aaaatattct atttatccgg cgtagaaaaa aagactgtga cacgcttttg tcacagtctt
    10381 ttttatatat aataatttaa aaattcatca tctaaatccg tagaattttc tagattgctt
    10441 acccatgtaa aatcgttata gacagatttc agttgacttc tgcgccattg tacagggata
    10501 aaaagacgca tgctatcttc attgaatccg actaataatt tctttccgtc agtaatgatg
    10561 ggacgttgta acaaagtaaa atgcgagacg atcagatcga caagctctga ggtcgtacat
    10621 tggtcgattt ggtctttcaa atttttataa tcaatcgagc gtttagctaa tagatcgtca
    10681 aatccgtttt ctgttaatga caataaataa aatatctctt tccttgttaa aggctgttta
    10741 ggtgtgaaaa ttcgttctct gtaaggcatt tgatggtctt tcagccagtt tgccatcaga
    10801 tggtaactat gtgtgcgaga agaatagatc gtaagcataa aaaaactcct taaatagtcc
    10861 attaatttgt tagatctgtt gagataaata ataggtaagg agctgtgcat aagcattgat
    10921 caaaacaacc caaacatcat tagaaataac tttctaaagt cttaaacttg cgacgagaag
    10981 caactagact aatagaaaaa atgtcttagc catttggctt cctgtttgtt tttttgctta
    11041 tgctgcagct cctcgggaaa gggttctaac aatttgaaga aggttgatga ttgatctagt
    11101 ttaaattgtg tgaaatttga aatctggtgt ttttactaga tgaatcacct aacaactatg
    11161 attataactt atagaacttc tgttttgcat tcggttttgt tatagttgtt ataaagtttt
    11221 attttattac agaacatttt taattaataa agaacagtta gaagttaaaa ttgctgattc
    11281 acatagagtt tatgacttct tggaaattct ttttcaatca gttttttcat ctgtttatga
    11341 aattcttttt tttcagtaaa cataaaggtt tgggtatcgt aaccttcttc atgtaggaaa
    11401 tagattattc ctttttctgt gttttgcgta taagctttta tactcctgtc ttttttgaaa
    11461 gaacagatcg tatagccatc tatttggtct ggtagtcgtt tgattttctt caaaatgact
    11521 tttttatcct caagtttcat ttttttcacc tgactttatt tttttcatat ggaaatcata
    11581 gcgcctttgg ttatatgaaa acaagaagga tactgaaata tatataaatc aaatagtggt
    11641 tatttattat tagaatagcg aatacaaata aacgaccact attttctgat ttattgcaaa
    11701 tgttttttca aataaatttg attaccaaga aaaggtagtg gtatatttac tgagggatac
    11761 tttgaaaaac atatgataga aacagtaaca gtattatgaa aaatcttttt ataaaaatgg
    11821 aggaatcgat ttgatggaag atcagtatgt acaagcaatg acgatcgcag gatcagatag
    11881 cgatggaagc gcagggatgc aagcagatat gcatacattc tttacaagaa atgtttacgg
    11941 tgtttctgtc atgaccgcat gtgttgcagg taattcttac ggaatcggag caagtgtgac
    12001 attgccaaca gattttatcg acaaagaatt tgaactgatt gcgaaggact ttcaaatacg
    12061 tgcagctaaa acaggtatgc tagcagattc aaagttgatt gagacagttg taaaaaatta
    12121 ccaaaaatat gattttggac cattagtcgt ggatcccgta atcgtaacga aacatggaaa
    12181 tcttttacta gaagaatctg ctttgcaaag cttgaaagaa aagcttgtcc ctttggccga
    12241 agtactgaca cctaattttt atgaagcaga aaagctgact ggacggacat tagcagctga
    12301 agaagatttt gtgcaagcag caaaagaact gcaagatatg ggtgcaaaaa acgttatgat
    12361 caaaggacat catgctaaaa atgcgcagca agaaacagtc aatgactacg tattgttcga
    12421 atcaggaaaa tcattttggt taaatgcacc attttatcct accgaccgga tcaacggaac
    12481 tggcgattcg ctatctgcat gtattgcagc tgagttagcg aaaggaaaaa cagttgaaca
    12541 agcgatccga ttggcaaaag actatgtaaa ccgtgcaatt cgtgaagaaa taaatgtagg
    12601 gcacaaatat ggccccatca atcattgggc agctggtgaa ttagagtaaa agaaagaagt
    12661 tgtgaaaaag ttgccctgca tcaagtaata agaacagcca agtaaaaaca gcttcttatg
    12721 tttttaactt ggttggactt attaccgcag atgcaagctt ttgaacaccg tttaagaaag
    12781 aggtcgtgag cctgccgtcc agctccgagt cacaaggaac aagttcaaaa aacagcttct
    12841 catgtttgtt gaatattgtg acttgtgacc gaggagttgg caggagaaca ccgtttgttc
    12901 ggaagaaggg actgtgacaa gatttttgtc acagtccctt taatcttagt atatttttct
    12961 ctacttgaat aaaaaaatta taggattgga gaaaataatc gagaaatttg ttgcttgaat
    13021 aatagccagt tagacatttc cttgactgtt tcagttgtca tggatgaaca atgtgtctca
    13081 tcttctttga actgagtagc aagttcttct aaaaactcag gatcgtaaat gacagcactt
    13141 gtttcaaaat tcaatcggta gctacgaata tcttgatttg ctgaacctac catgcagatt
    13201 tcatcatcca ttatcaatgt ttttgcatgg aggaagccgt tctcatagac aaggatttgt
    13261 acattttcct tgatcagctg ccgagcataa tattgtgttg ctcgataaat aaaaggatga
    13321 tccggtttgt tgggaatcat gattttcaca tctacaccag aggcagttgc gatttttaaa
    13381 gcagcaatga cactttcatc aggaacaagg taaggtgtct gtatccaaac acgtttttta
    13441 gcagaagtaa tcaatttgat aaatgaaagc ttgatttgtt ccctttcgtt attcggtccg
    13501 cttgaaacaa gctggatgga tgtatcagca agcttagatt catctcgatc tgctttttta
    13561 aagaaataat tcaaatgata gccgaccttt ttttcttcag gtaccgagac gttccagtct
    13621 gttaagaagc gaagctggag caaagaagaa gcggcaccga aaatccgtat atgcgtatcg
    13681 cgccaatagc cgaatttttt tgttgtattt acatattgat tggcaatatt gaaaccaccg
    13741 gtataactaa tctttccatc gatcacaacg atttttcggt gatcatggta attcaatcgg
    13801 aaacgaagta atgccctttg tgaggtaaca aatgtatgga catgtccacc atttttgatt
    13861 agacgattga aatcttttgc ttttgtgcca tgagagccaa atgcatcata taatattcga
    13921 acttccacgc cttctgcggc tttttcttct aaaacatgta agacttgctg gccgatatta
    13981 tctgtcacaa atgcgtaata ttcgatatgg atcgaatgtt tggctttttt tagatcttga
    14041 agcagtgaat ccaatttctc ttgtccgtct gtgagaagag tgacagaatt cattcttgtc
    14101 agcggcatac gatttaatga agagaagaag tcaacaaatt gctgtttgtc tttatcgccc
    14161 atgtcaggat cgtaatgttc aatggtatct cctctaaaag actgaaagtt ttctaattct
    14221 ttcaagtcac tttgtcggag atagaatttt ttcttatccg ttaatccacg tccgaaaaat
    14281 aaatataata caaagcccac cccaggaagg gcaaacaata ctaagagcca tgcccaaatg
    14341 gctgctacat ctcggggttt gatcaaaata gccaccaaag caataagtgc atttaataga
    14401 taaagggcgg ttataatact agataccaca aaaatgttcc tccttacata caataacatt
    14461 agtatagcat gatagaagaa agaagcctac caaggtgaat atacttttca ctcctttcat
    14521 gaaaatgtag gtaaatcggg ctaaagtagt tgctttttgt aggttaataa ggtaggatat
    14581 tagtacagtt gaatatctcc ttaggggagt aaccggcaac gtttgttgcg ataatatcaa
    14641 cagtaagtta accaaatggt tttctggtat tgtatgaaat ggtgagacct aagacaaatg
    14701 cgtatttgtc ttaggtctct tttttttcag acaggactag ggccaagatc aaaaaatggg
    14761 ggatgaaatt tggaaaacaa ggaacaagca caacaagcgg tctataaaga aacgatcgac
    14821 tcgttgatgc agcaaacgca gagctcaacc gatggattga gcaccacgca agcgaaggaa
    14881 aaattagaaa gagatggctt gaaccagcta aaagaaaagc cgaagaagcc aacttggaaa
    14941 ttgttcttag aaactttcaa agatgcaatg gttatcgtat tgctgattgt cgcactgatt
    15001 cagatggtca tgggtgacta catcgaattc ttagttattt ttgcagtatt gatgttgaat
    15061 tctataatca gtgttgtcca aacaaaaaaa gcagaaggtt ctttagaagc gttgaaaaac
    15121 ctttcagcgc ccgatgctcg agtgattcgt gatggtaagg aaaaaacgat tccggcaaac
    15181 gaacttgtgg taggagatat cgtgttgtta gacgcaggcg attatgtacc tgctgatgga
    15241 cgtttactag atgcgggatc attaaaagta gatgaaggta tgctgacagg agagtcgact
    15301 tctgcagaaa aagaagtaac ggatatcaca agtacggtac caatcggaga tcgtacaaat
    15361 atggtattca gtggaacgat cgtaacttac ggacgcggta aattcttagt gacttcaact
    15421 gggaataata cggagatcgg acaagttgct ggccttttgg aatcaacaac tgagcaagtc
    15481 actccgttac aacgaggtct ggataaattc agtaaaaaac taagtttggc tattttggca
    15541 ttgtctttat tgattttagg tatccaactg tttcgtattt atcttggaga aggtacagga
    15601 gatatgactg cagatatcgt cagcgcagtg atgtttgccg tagccgtagc cgtagctgct
    15661 attccagaag cattacagtc tatcgtaaca atcgtgttat ctttgggaac gaataaaatg
    15721 gcaaaacgtc atgccattat tcggaagctt ccagcagttg aaacacttgg ttcgacaagt
    15781 atcatctgta cagataagac tggtacattg acacaaaaca aaatgacagt tgttgactac
    15841 tttttaggta atggcgatac tggggatttc acaaatgacc cttcaaaatg gacagctgac
    15901 gaacgtcgct tgatcgaaat cagtgtttta gcgaatgatg catcgatcag tgaagacggt
    15961 actaaattgg gcgaccctac ggaagtagcg tttatcgact ttagtgaaaa attaaaccag
    16021 ccttatcagg aaatccgaaa caactaccca agacaagcag aacttccttt tgactcagat
    16081 cgaaaattaa tgtctactgg acacacaatt gataacaaaa attacttgtt tgttaaaggc
    16141 ggaccagata tcgtcttcca acgcagtaaa aaagtattgc taaatggtga agtcgttccg
    16201 atgacggatg aattgatgaa aaaattccaa gagcaaaatg aagctttttc aacacgcgca
    16261 ttacgtgtac tagcttttgc ttaccgtcca cttgataatt ttgatttatc atttgaagat
    16321 gaagacgatt tgatccttgt tggtttgctt gctatgatcg atcctccacg tgctgaagtg
    16381 actcaggcgg taacagaagc aagaagtgca ggaatcaaaa caatcatgat tactggtgac
    16441 cacaaaacaa cagctgttgc tatcgcaaaa caaatcggga tttttgaaga aggaaacctg
    16501 gctttgaccg ggacggaact agatgaattg acagaagatc aactaaacga agttttagaa
    16561 aaagtcactg tttatgcacg tgtttcacct gagaataaaa ttcgtatcgt tcgtgcttgg
    16621 caaaataaag gtcatatctc agctatgaca ggtgatggtg taaatgacgc accagcattg
    16681 aaacaagcag acatcggaat tgctatggga acgggtacag atgtggcaaa agatgcagca
    16741 gcgatggtat tgacggatga taactttgct tctattgtca gcgcagtaga agtaggacgt
    16801 aatgtgtttg ataatatcaa gaaagctatt tcttatctat ttgcaggaaa tttaggtgcg
    16861 atcattgcga tcatctttgc cttagttgct gactggtcca atccatttac agcattacaa
    16921 ctgttgttca tcaatttagt caatgattca ctgccagcaa ttgcattagg tacagaaaaa
    16981 gctgaacctg ctacgatgaa ccgcccgcca agagatccaa attcaggaat ctttacggac
    17041 aaaacattga tttctgtaac gtatcgtggt gtgctgattg ggattgctgt tatctttgct
    17101 caatggcttg gcatgcagca ttcacctgaa ttcggtgtag ccatgtcatt ttcaacattg
    17161 atttggtgtc ggacattgca aacattccca gcacgttcaa acacacaaac agctatccaa
    17221 gcagggttct tctctaacag aagtgtctta ctagctgtag ttgtatgtag cgcgctttac
    17281 tgcttaacaa tgatcccagg actgaaagaa gtcttctcta tcccatcagc atttggattg
    17341 acagaagtct ttacatgtat gggaatcgca cttgtgacaa ttgttttgat ggaaatcact
    17401 aaattgatca ttgtacgagt acaaggaaat aaataaaacg aatcaacgag gttgtgagcc
    17461 tgccgtttaa ttctgagtca caaggtgcaa tttcaacaaa cagcttctca tgtttgttga
    17521 atattgtgac ttgtgaccga agagttggca ggagaacacc gtttaaggaa gaagttgtaa
    17581 aatcagcatt ttgagctaat actgagggtc atctgattgg acactgttta ttcggaaaga
    17641 gggactttga cacgactttg gtcacagtcc ctttttttat ttatgataga cggaaagcca
    17701 atcattttat tattcatcaa aagagataga atagataaaa gaggagaatg atttattccg
    17761 aaaagtgaaa acatataaca aaaaattaga ggaaatcaaa tgaatgaaaa agaatgggat
    17821 gaatttgctg aagaatatta tgaaatccag caagaatcgc aagtaactat tgctgaagat
    17881 gtaaaaaatt atctgaaaac ggagaaagtc ctgcctgctg aaacagcagc agacgttgct
    17941 ggaggatctg gcagatttct ttccttagca aaagaaatta atcgctatga tttgatcgat
    18001 ttttcttcag gaatgctaga aaatgcaaaa aaagaagcag aaaaaaatga gttgccaaat
    18061 gtttccttta tcaaacagtc gttagaagaa tttatccaat caaatgagac atatgaaatg
    18121 gtttttacag cagcaaatcc agcattggta catcttattc aattagaaaa attgatgaaa
    18181 aagtcaagca aatggtgcgt gataatcaga gtagtggaat caatcgatga tgttttttat
    18241 cctatcgaaa agcaactagg aatgatagaa gaagatccta atacatcgcc agttgtaatg
    18301 gatcaatttg aaacatactt aattgatgaa ggattcaaag tgaagaaaaa agattttact
    18361 tattttacac aggaatcaat tactgaagaa ttgatgaagc tatactatga cgcgtacgca
    18421 aaagatcctg ttgtgaccag gtacattcgc aaactattca gtgaaaaacg agaaatcctt
    18481 agtacgacta agctgaccta tagactgctc ctttggtccg cagataaaaa aagaaccttt
    18541 cacgactcgt gaagggttct ttttatgatt ttattatatt ttgtagtaac gaatatcatt
    18601 ttcgatcttt gtaatcgaaa aaggtacatc tgaagtatat tctgcacatt ctccgtgttc
    18661 aggaagcgtc tcactgaaaa tcgtttcata ttcgtcaaca gtgatccgtg ttcgagaatc
    18721 tagcatctct tgatgagatt gtgcgaagag atattcttga tagttttctt ccagataacc
    18781 tgtaaaaaat tcacttactg caccagaacc gtagctgaat agaccgatgc gatctcctgg
    18841 ctgtagtgat ttcgaatttt ccagtagaga agttagcccc aggtataatg aaccagtgta
    18901 aagattaccg attcgtcggc tgtaacggat gctttcttca tagcgagcca taagacgttc
    18961 ctgattttct tcgtctgttt ggtctaatac gctttgcaat gccttttttc ccatcttagt
    19021 atacggaata tggaaagcaa tcgcctgata atcttcgagt cctcgacccg acaattcttt
    19081 atgtcgattc caaacttttt ggaatgattc gatatacgta gaattagata aaggaccatc
    19141 aacaacagga aattcgctat aatctggacg ccagaaatca tagatatctt ctgtcagaaa
    19201 tacgctgtcg tcttcaatcg ataaaatacg cgggttttga gtgatcatca tcgcaacagc
    19261 accgacacct tgcgtcactt caccaccgct tgccaagctg taacgagcaa tatcacttgc
    19321 tatgactagt acttttcgtt ctggatgatt tttgacatat tctttcgcca tgtgcaaagc
    19381 tgcggtgcca ccgtagcaag cctcttttac ttcaaaagaa cgagcaaaag attggatttt
    19441 cagtaaatgg tggataatta ccgcactcgc ttttgaatga tcaatcccgg attctgttcc
    19501 gacgatgacc atgtcgatta gctcacggtc tttttctgtt acgattttgc tggcagcatt
    19561 tgctcctagt gtaatgatgt cttcatttgc acgattgact gccatttggt cttgaccaat
    19621 ccctatatgg tattttgcag gatcatcccc acggctttct gccaactctg tcatatctaa
    19681 atataaatta ggaataaaaa aggaaagacg atcaatccct attttcattt caacacctct
    19741 ttacttatga taaaaaactt actttactaa ggtatcataa aaagattaaa agaaatataa
    19801 aaaagagaat atttaaggat tgttaaaatt taatcttaga aaaaaataac tggaacaaga
    19861 gctatttgtt tttacagcaa agaatggtat atttttgtag gaggtggctt acttgaaaga
    19921 agtcgttatg atagatgctg caagaacgcc gataggaaag tatcgcggaa gtttaagtcc
    19981 atttactgca gttgaattag gcacacttgt gactaaagga ttattagata aaacaaaact
    20041 gaaaaaagat aaaatcgatc aagtaatatt tggaaatgtc ctgcaagctg gtaatggtca
    20101 aaatgttgcc agacagattg ctttgaacag cggtctccct gtagatgtcc cagcaatgac
    20161 gatcaatgaa gtttgcggat ctggtatgaa agcagtgatc ttagcacgcc agctgatcca
    20221 attaggtgaa gcagaacttg tgatagccgg cggaacagag agtatgtctc aagcacctat
    20281 gctgaaacag tatcagtcag aaacaaatga atatggtgaa ccaatttcca gtatggtcaa
    20341 cgacggattg actgacgcat tttcaaatgc acatatggga ttaaccgcag agaatgttgc
    20401 aacacaattt tctgtgagca gagaagagca ggatcgctat gccttgtcgt cccagttgaa
    20461 agcagcacat gctgtcgaag ccggtgtatt ttctgaggag atcatcccag tcaagatttc
    20521 tgatgaagac gtgttatctg aggatgaagc agttcgtgga aatagtacat tggaaaaact
    20581 gggcacgtta cgtacagtat tctcagaaga aggaactgta acagcaggaa atgcttcccc
    20641 gttgaatgac ggtgcctctg tggtgatcct tgcatccaaa gaatacgcag aaaataataa
    20701 tctgccttat ttagcaacca tcaaagaagt agcggaggtc ggtattgacc caagtatcat
    20761 gggaatcgct ccaatcaaag ccattcaaaa attgacagat agatcaggaa tgaatttatc
    20821 aacaatcgat ctttttgaga tcaatgaagc atttgcagct tcttctatcg tagttagcca
    20881 agaactgcag ttagatgaag aaaaggtcaa tatatatgga ggagcaatcg cattaggcca
    20941 tccaattggt gcaagcggtg cacgtatctt gactacatta gcttacggat tgcttcgtga
    21001 acagaagaga tacggtattg cttcattatg tattggtgga ggtttaggct tggcagtctt
    21061 gttagaagcg aatatggagc aaacacataa ggatgtgcaa aaaaaaaagt tttatcagct
    21121 tacgccatct gaacggcgca gtcaattaat agaaaaaaat gtattgacac aagaaactgc
    21181 gttgattttt caggaacaaa cactttctga agagctctcc gatcacatga tcgaaaacca
    21241 agtgagtgaa gtggaaatcc caatggggat cgcgcagaat tttcaaatca atggcaagaa
    21301 aaaatggatt ccgatggcaa cagaggaacc atctgtcatt gcagctgcaa gtaatggtgc
    21361 aaaaatctgt ggaaatatct gcgcagaaac acctcagcgg ctgatgcgag ggcaaatcgt
    21421 tctatcagga aaatcggagt atcaagcagt gatcaatgca gttaatcatc gaaaagaaga
    21481 gctgattctt tgtgcaaatg aaagttatcc ttcaatcgtg aaacgcgggg gcggtgttca
    21541 agatatctct actcgggaat tcatggggag tttccatgca tacttatcta ttgatttttt
    21601 agtggatgta aaagatgcaa tgggcgctaa catgatcaac tcgattcttg agagtgtggc
    21661 aaacaagtta cgggagtggt tccctgaaga agagattcta ttcagtattt taagtaattt
    21721 tgctacagaa tcattggctt ctgcctgttg tgaaatccca ttcgaacgct tagggagaaa
    21781 caaagaaatc ggtgaacaaa ttgccaaaaa aatccaacag gcaggggaat atgcgaagct
    21841 tgacccttac cgtgcagcaa cgcacaacaa agggatcatg aatggtatag aagctgtagt
    21901 ggcagctaca ggaaatgata cacgtgcggt tagcgcttcg atccacgcct acgcggcaag
    21961 aaatggatta taccaaggat taacagactg gcagatcaaa ggggataaac tagttggaaa
    22021 actaacggtg cctttggctg ttgccacagt aggcggagct tccaatatcc tgccaaaagc
    22081 aaaagcatct ttagctatgt tagatattga ttcagccaag gaactagcac aagtaattgc
    22141 tgcagtaggg ttagctcaaa acctggctgc tttgcgtgca ttagttacag aaggaatcca
    22201 aaaaggccac atgggacttc aagctcgttc attagcgatc tcaattgggg ctattggtga
    22261 ggaaatcgag caagttgcga aaaaattacg tgaagcagaa aagatgaacc agcagacagc
    22321 tatacaaata ttggaaaaaa tccgggaaaa ataaaaaagc aatatcttta gaagaataaa
    22381 gcaagattta cagttatttt atttactgca gatcttgctt ttt
//
LOCUS       DASHHB010000042        22382 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.043, whole genome shotgun sequence.
ACCESSION   DASHHB010000042 DASHHB010000000
VERSION     DASHHB010000042.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 22382)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 22382)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..22382
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.043"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            12..84
                     /locus_tag="U2K71_001857"
     tRNA            12..84
                     /locus_tag="U2K71_001857"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:45..47,aa:Val,seq:tac)
     gene            88..160
                     /locus_tag="U2K71_001858"
     tRNA            88..160
                     /locus_tag="U2K71_001858"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:121..123,aa:Lys,seq:ttt)
     gene            168..249
                     /locus_tag="U2K71_001859"
     tRNA            168..249
                     /locus_tag="U2K71_001859"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:202..204,aa:Leu,seq:tag)
     gene            267..339
                     /locus_tag="U2K71_001860"
     tRNA            267..339
                     /locus_tag="U2K71_001860"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:300..302,aa:Thr,seq:tgt)
     gene            351..422
                     /locus_tag="U2K71_001861"
     tRNA            351..422
                     /locus_tag="U2K71_001861"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:383..385,aa:Gly,seq:gcc)
     gene            439..524
                     /locus_tag="U2K71_001862"
     tRNA            439..524
                     /locus_tag="U2K71_001862"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:473..475,aa:Leu,seq:taa)
     gene            535..608
                     /locus_tag="U2K71_001863"
     tRNA            535..608
                     /locus_tag="U2K71_001863"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:569..571,aa:Arg,seq:acg)
     gene            618..691
                     /locus_tag="U2K71_001864"
     tRNA            618..691
                     /locus_tag="U2K71_001864"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:652..654,aa:Pro,seq:tgg)
     gene            711..783
                     /locus_tag="U2K71_001865"
     tRNA            711..783
                     /locus_tag="U2K71_001865"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:744..746,aa:Ala,seq:tgc)
     gene            811..884
                     /locus_tag="U2K71_001866"
     tRNA            811..884
                     /locus_tag="U2K71_001866"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:845..847,aa:Met,seq:cat)
     gene            898..971
                     /locus_tag="U2K71_001867"
     tRNA            898..971
                     /locus_tag="U2K71_001867"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:932..934,aa:Ile,seq:cat)
     gene            995..1084
                     /locus_tag="U2K71_001868"
     tRNA            995..1084
                     /locus_tag="U2K71_001868"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1031..1033,aa:Ser,seq:tga)
     gene            1097..1170
                     /locus_tag="U2K71_001869"
     tRNA            1097..1170
                     /locus_tag="U2K71_001869"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1131..1133,aa:Met,seq:cat)
     gene            1189..1264
                     /locus_tag="U2K71_001870"
     tRNA            1189..1264
                     /locus_tag="U2K71_001870"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1222..1224,aa:Asp,seq:gtc)
     gene            1266..1336
                     /locus_tag="U2K71_001871"
     tRNA            1266..1336
                     /locus_tag="U2K71_001871"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1298..1300,aa:Gly,seq:tcc)
     gene            1350..1423
                     /locus_tag="U2K71_001872"
     tRNA            1350..1423
                     /locus_tag="U2K71_001872"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1384..1386,aa:Ile,seq:gat)
     gene            1448..1535
                     /locus_tag="U2K71_001873"
     tRNA            1448..1535
                     /locus_tag="U2K71_001873"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Ser,seq:gct)
     gene            1557..1628
                     /locus_tag="U2K71_001874"
     tRNA            1557..1628
                     /locus_tag="U2K71_001874"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1590..1592,aa:Glu,seq:ttc)
     gene            1651..1740
                     /locus_tag="U2K71_001875"
     tRNA            1651..1740
                     /locus_tag="U2K71_001875"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1687..1689,aa:Ser,seq:tga)
     gene            1753..1826
                     /locus_tag="U2K71_001876"
     tRNA            1753..1826
                     /locus_tag="U2K71_001876"
                     /product="tRNA-Met"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1787..1789,aa:Met,seq:cat)
     gene            1845..1917
                     /locus_tag="U2K71_001877"
     tRNA            1845..1917
                     /locus_tag="U2K71_001877"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1878..1880,aa:Asp,seq:gtc)
     gene            1921..1993
                     /locus_tag="U2K71_001878"
     tRNA            1921..1993
                     /locus_tag="U2K71_001878"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1954..1956,aa:Phe,seq:gaa)
     gene            1998..2068
                     /locus_tag="U2K71_001879"
     tRNA            1998..2068
                     /locus_tag="U2K71_001879"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2030..2032,aa:Gly,seq:tcc)
     gene            2081..2154
                     /locus_tag="U2K71_001880"
     tRNA            2081..2154
                     /locus_tag="U2K71_001880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2115..2117,aa:Ile,seq:gat)
     gene            2166..2237
                     /locus_tag="U2K71_001881"
     tRNA            2166..2237
                     /locus_tag="U2K71_001881"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2199..2201,aa:Glu,seq:ttc)
     regulatory      2426..2505
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF01767"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="SAM riboswitches modulate the expression of
                     downstream genes in response to levels of
                     S-adenosylmethionine. SMK box riboswitch (aka SAM-III)
                     shares no known similarity to SAM-I and SAM-II; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF01767"
     gene            2503..3696
                     /locus_tag="U2K71_001882"
     CDS             2503..3696
                     /locus_tag="U2K71_001882"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HEM7315936.1"
                     /translation="MVERHLFTSESVSEGHPDKIADQISDAILDAILKQDPTARVACE
                     TSVTTGLVLVFGEISTTAYVDIQKVVRETIKEIGYTRAKFGFDGDTAAVLVAIDEQSP
                     DIAQGVDEALEIRDEDKKDVLDEIGAGDQGLMFGFAVDETPELMPLPIALSHRLVRRL
                     ADLRKSNELTYLRPDAKSQVTVEYDDQGQPERVDTIVISTQHDDAVDNETIRHDVIEK
                     VVKEVIPAELLDDQTKYYINPTGRFVIGGPQGDAGLTGRKIIVDTYGGYARHGGGAFS
                     GKDATKVDRSASYAARYIAKNIVAAGLARKAEVQLAYAIGVAQPVSISINTFGTGTVP
                     EEELIAAVRENFDLRPAGIIEMLDLRRPIYKQTAAYGHFGRTDVDLPWEHTDKVDALK
                     ASLAK"
     gene            3826..5298
                     /locus_tag="U2K71_001883"
     CDS             3826..5298
                     /locus_tag="U2K71_001883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7315937.1"
                     /translation="MKKETNVTLVTVSIFIATFMTAIEGTIVTTAMPTIVGSLHGMEI
                     MNWVFSIYLLTNAMLTPIYGKLADKVGRKPIFIFGTLLFIIGSLFCGLSQSMIGLIAA
                     RAIQGMGAGAMMPVALTIIGDLYSIEKRAKVLGLNSSAWGIASVFGPLAGGFIVETIS
                     WHWIFFINVPIGILLIILIWLYLNEPKIIHESKPMDIFGSFSLMAMLVSLLLGFQLLG
                     DEGMSPLVSALLLGSIVMLGIFVFAEKRAKDPVIMMHLFKNPLFVVVNLAAALASGFL
                     MGIDVYIPMWMQGVLGLNAAIGGLVLAPLSLVWMVGSFLASKWMTQMKTHWVLRIGLL
                     IAAIGGAWLYLMPFRSNYLWFFAISTVIGIGLGVTMTTSTVSAQNSVSRDQLGVATSF
                     NTLVRTIGQTVMVSIFGLLINGVTKRELAAANLTNDADIMNKLVNPQTAKYIDADILL
                     PLRKILYDGLHSVYLVGLLLVIIALVLTLFVHEKSGKIKQ"
     gene            5381..6169
                     /locus_tag="U2K71_001884"
     CDS             5381..6169
                     /locus_tag="U2K71_001884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="esterase family protein"
                     /protein_id="HEM7315938.1"
                     /translation="MAFIQANIYSNVLEMEVNLNVILPQTTKKVIGTSSEQETTDIPV
                     LYLLHGMGGNHSVWERRTSIERYVANYGLAVIMPSTDLGWYTDTVYDMKYWTFIAEEL
                     PEICHELFPQLTRKREKTYAAGLSMGGYGALKLGLAKSENFAAVISLSGAVSVGNRMD
                     DLLMVRKASFWEGIFGPLDQIEGSKNDPQYLLKELVKSGKPVPRFYMACGESDFLYQA
                     NQEMVSAMQEQGLAVTFEQGPGDHNWIFWDQWIQRALAWLMEEK"
     gene            complement(6236..6904)
                     /locus_tag="U2K71_001885"
     CDS             complement(6236..6904)
                     /locus_tag="U2K71_001885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator
                     C-terminal domain-containing protein"
                     /protein_id="HEM7315939.1"
                     /translation="MEIKLSQAIIIQAAFDILAETQALSQLSMRNIANRIHVQAPALY
                     WYFKNKQQLLQKMAETMEEELALPDPTLPWNEQLLQYMENYYDLYTRFPCGAELEINT
                     VPAFPSRLEHLEQMIQLLVREGFSVTQSHQAIASLHNLLIGQLMDQQHEQQLRQKIMD
                     GNEFLKETVLFMRNYVVENQLEGFKESMQSHAELNEKALFLTSAQTYIEGLCQQNKRR
                     ENEQ"
     gene            7019..8731
                     /locus_tag="U2K71_001886"
     CDS             7019..8731
                     /locus_tag="U2K71_001886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315940.1"
                     /translation="MKLILRHAKKYKVSVFISLLSVAVMVAAALWQPKLLQQVLEAII
                     TEDNDEMKTIGIYLISLALLGLAAGVTNTIFSAKVAQGVSADIREEAFRKIQTFSFGN
                     IEQFSAGNLVVRLTNDITQIQNLVMISLQSLFRIPFLFIGAFILAMITMPQLWWIIVA
                     LIVTVFLITALSFTRMGKHFMIIQKLIDRVNSIAKENLLGIRVVKSFVQEENQLKSFS
                     KVSEDLTKHNIIVGTLFSVMIPSFMLAANLAVVGAIFFVSDLVKDDPTLIGGIASFMN
                     YLMQIMMAIIIGGMMMMMTSRAAVSLKRIAEILDAEPDLTYLDVPEQELTGSVTFDHV
                     SFRYPGEDTDTLKDISFSIKPGEMVGIVGATGAGKSTLAQLIPRLFDPTEGKVEVGGV
                     DLRQVNEKSLRQTVSFVLQKAILFSGTIAQNLRQGKKNATEKEMEHASSIAQAKEFIE
                     KLSDRYEAPIEERSSNFSGGQKQRLSITRGVIGNPKILILDDSTSALDARSEKLVREA
                     LDRDLKGTTTIVIAQKIASVVKADRILVLDEGRLVGEGTHEELVAGNRVYQEIFETQK
                     GTEE"
     gene            8734..10506
                     /locus_tag="U2K71_001887"
     CDS             8734..10506
                     /locus_tag="U2K71_001887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299072.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7315941.1"
                     /translation="MTDLIKASKFFYHYLKRYKLSFFFIFITVIIATYLQVKAPQYIG
                     EAFQELANYVGGLMQGVDDKSKFVSVIWKLLLFYVLASAANFIYSILFTQVVGKSTNR
                     MRIGLFNKLEKLTIRFFDSHQDGEILSRFTSDLDNIQNSLNQALLQVITNAVLLVGIL
                     IMMFRQNVELAWATIASTPVAVLIAVLIIRKARKYVDLQQDEVGKLNGYMDEKISGQR
                     VIITNGLQEETIEGFLEHNQSVREATFKGQAYSGLLFPMMQGMSLVNTAIVIFFGGWL
                     ALNGDLERSVALGLVVTFVQYSQQYYQPLMQISSGYSMIQLAITGARRLNEIFDEKDE
                     VKPENGKAFDGVEKGLALQHVDFGYSPEKLVLKDVSINVDKGEMVALVGPTGSGKTTI
                     MNLLNRFYDVNDGSVVIDGIDIRDYDLDALRRHVGIVLQESVLFSGTIKDNITFGKPD
                     ASDEEVINAAKQANIHDFIMTLEKGYDTEISEENNLFSTGQKQLISIARTIITNPSLL
                     ILDEATSNVDTVTEARIQKAMDEAIKGRTSFVIAHRLKTILNADRIVVLRDGEVIEEG
                     SHHELLAENGFYAELYHNQFVFES"
     gene            complement(10588..11709)
                     /locus_tag="U2K71_001888"
     CDS             complement(10588..11709)
                     /locus_tag="U2K71_001888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM7315942.1"
                     /translation="MWLEKINDKKFKYTERYTDPLTEKKRKVSVTLSSNSRQAWNQAN
                     LLLNEKIAEKIKRNEELPLTFGELKTKWDEKYKPTVKESSYRTTQVYLSLISKYIKDD
                     VLVKNVNSNLIQDMLDYYYFEKNLSYNYVIHLKTFTGMIFKFANRRYGLKYNPVNGVS
                     LARKPKTNEEIKKEKDGYLSKEEVQMIASKQKSSHQQSRYSLITKFLFLTGLRYGELI
                     SLTESDYDGNKITVSGTYDYELKIKTTTKNTGSYRTIELSSNAKEIIDQLIEENKLIK
                     NNKDKYIFISKNGNPISIQAYNQSLRAVSKELNLDKKVSSHMLRHSHISLLTELGIPL
                     KAIMDRVGHEDSKTTLKIYTHTTKNMQNQLVEKLGKIEI"
     gene            complement(11781..13061)
                     /locus_tag="U2K71_001889"
     CDS             complement(11781..13061)
                     /locus_tag="U2K71_001889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4041 domain-containing protein"
                     /protein_id="HEM7315943.1"
                     /translation="MLLLIGLILLIIGITKLSKANTIKKEAYELEQKNDEKLNKLHDR
                     ERFILSKEETLSDLNKQITMLKNDIILLKKEKEDLNDEINQKYDETLKNVYSSLIVSN
                     IEETSSSEIKNKLSLLKLKEKEYIIDDAVSVPLILNKREKNNLKKKMLIPFDSEVKGL
                     LNKLTISNIDSTREKIIKIFDKINNLFKNDNAELRKGLLEFKLQELELHYSYLVKITE
                     EKEQQKAIKEQMIEEEKVRREIDREKKRIDKEQRQFNSEISKLIAYMQKANADVEKEL
                     YANKIQELEEKLKELEVIRENVLQRELNTRAGYVYVISNIGSFGEDIYKIGMTRRLEP
                     MDRIKELSSASVPFEFDVHAMIFSEDAPSLETKLHNHFRKQEVNKINQRKEFFKVSLD
                     EIEKVVLENYNGTVTFTKLAKAEQYRRSLELSKD"
     gene            complement(13152..13604)
                     /locus_tag="U2K71_001890"
     CDS             complement(13152..13604)
                     /locus_tag="U2K71_001890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM7315944.1"
                     /translation="MHLNKYWILEEIEKLFVEFGSDAINPYKIIKYSDIDLIETDLGN
                     KTLGQTIHNKRCYVILINQNLSPSMKRFVLWHEIAHVRLHKGICTAAFRANNLSCMIY
                     GIEAEANMFAIEMLTRTIDEQDLSDMSQFQIIEYLGLPHNLEHLVLQH"
     gene            complement(13617..14027)
                     /locus_tag="U2K71_001891"
     CDS             complement(13617..14027)
                     /locus_tag="U2K71_001891"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315945.1"
                     /translation="MLLDRIKILLKERSMTLAELERKLNFGNGSMSRWNKSTPSGDKI
                     QKVADYFNVSTDYLLGRTDKRHYYDLTEKDKQDIALQAEKLLEGLNSDAETNYYGEPL
                     DDEDKEKLYDAIQLALALTKVKAKKKFTPKKYRQ"
     gene            14302..14514
                     /locus_tag="U2K71_001892"
     CDS             14302..14514
                     /locus_tag="U2K71_001892"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7315946.1"
                     /translation="MTLKQQVLIALVKKGWSQRELARRMGISITYLRDIFIEKRKPKE
                     RLKQIEELLDIKLEVDSNDQPSEQEA"
     gene            14517..14729
                     /locus_tag="U2K71_001893"
     CDS             14517..14729
                     /locus_tag="U2K71_001893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315947.1"
                     /translation="MVQRLSKAEFKEKYKYSESTYQRRMKEFKKSRFSEGYNAVTSKE
                     ITIDVDLYEQFRTWRSKNRFRVRAVC"
     gene            14869..15024
                     /locus_tag="U2K71_001894"
     CDS             14869..15024
                     /locus_tag="U2K71_001894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315948.1"
                     /translation="MKEESKIGILNENNYTRLIIETEEGKKVAEVTLTDATPADGYRI
                     RLTPKYD"
     gene            complement(15021..15245)
                     /locus_tag="U2K71_001895"
     CDS             complement(15021..15245)
                     /locus_tag="U2K71_001895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2188 domain-containing protein"
                     /protein_id="HEM7315949.1"
                     /translation="MGKNQHVVPNSNGGWNVKGAGNSKATAHTNTKSEAVKIAREISK
                     NQGSELFIHGRDGKIQSRDSHGNDPHPPKG"
     gene            15323..15511
                     /locus_tag="U2K71_001896"
     CDS             15323..15511
                     /locus_tag="U2K71_001896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315950.1"
                     /translation="MAYTLQQEHQILGLIKQRRKQLQDDRAALRKADELSDRQAELIA
                     SELEDLRMLEIKNREIRL"
     gene            15508..15681
                     /locus_tag="U2K71_001897"
     CDS             15508..15681
                     /locus_tag="U2K71_001897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315951.1"
                     /translation="MKKTDTLFIGFILGLLVIVAHQSIIGGSLFAALMVLINLLDSKE
                     RSNYGTRRSAKNR"
     gene            15837..16172
                     /locus_tag="U2K71_001898"
     CDS             15837..16172
                     /locus_tag="U2K71_001898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315952.1"
                     /translation="MNKKIENLIEELKRECQKQGVSIICTAQKEGELKSLVYGETTEI
                     LLCLAMQEEHLDENLPLSAHIMRRIAVDAYEQAKNEEENQPSNHTFVINNKEDLADVM
                     TRILKGEFQ"
     gene            16405..17346
                     /locus_tag="U2K71_001899"
     CDS             16405..17346
                     /locus_tag="U2K71_001899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqaJ viral recombinase family protein"
                     /protein_id="HEM7315953.1"
                     /translation="MSKSTLEMSHQEWLEDRKKGIGGSDVGTILGLNKWKSPYQLWLE
                     KTGQVVLEESGSEPAYWGNVLEEVVAKEFQERTGKKVRRRNQVFEHPLHPFLRANIDR
                     EVVGENAILECKTANQFLGKEWEGEEVPLSYLCQVQHYMNVLNKDYCYIAVLIGGQKF
                     IWKRIERDQELIDTITEQLVEFWETNVLGGIEPIIDGSQATADFLKEKYADVEDVQTT
                     LPIHFDELVEQKNELKRTKKEIESAIRQVDNEIISELGKREASIGITQKNIISWKFVR
                     TRRMNSKKLAEKYPDVANDEEIYNVTESRRLTEREIK"
     gene            17348..18238
                     /locus_tag="U2K71_001900"
     CDS             17348..18238
                     /locus_tag="U2K71_001900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecT"
                     /protein_id="HEM7315954.1"
                     /translation="MATNESLKNQLAAKPQKQVAPGQLGLKALMNTPTMRKKFEEVLH
                     DNANAFMSNVMTLVSNDSYLADSEPMSIMSGALTAATLNLGLDKNLGYAYLVPFNSKN
                     KQTGKWEKKAQFMLGYKGYIQLAQRSGKYKALNVIEVYEGELKSWNRLTEEFEFDPNG
                     RTSDEVIGYVGYFELLNGFKKTVYWTKQEIEAHRIANNKDRDKTKLSGVWASDYNAMA
                     RKTVLRNLLSKWGILSIEMQEATTSDERVQRVQEDGSIIAETEVEEDIPERKEAEVIS
                     EENEDVQTGLFDASNPPLNK"
     gene            18281..19081
                     /locus_tag="U2K71_001901"
     CDS             18281..19081
                     /locus_tag="U2K71_001901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication protein"
                     /protein_id="HEM7315955.1"
                     /translation="MNTGYVKLHRKVMNSFVWTNPYMYKLWNLCLMKASHENRKILFN
                     GKEIQVNSGEFVTGRDAITSEMNEGVKREHQVNSGSVWRWLKQFEKNGMLNIKSTTKY
                     SVISIKNWSLYQSSEQQMNIKRTTSEQQVHTNKNEKNYKNEKNNKTSSSQPRKKRVYD
                     TDSVYYILAEELFKQICQNQEIKKPNLQSWADDIRKMIEIDKRTENQVRGMIEWSQHN
                     VFWASNILSAKKLREKYDTMAAQANRDYKTKQTKTLEYEKFSTDELPI"
     gene            19096..19947
                     /locus_tag="U2K71_001902"
     CDS             19096..19947
                     /locus_tag="U2K71_001902"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM7315956.1"
                     /translation="METVGEIMEKLIQKVLVQRGECPECGQPLYGWRTKNPDGSERCK
                     PTCMQCGYKALRVQEDLQTERIYNESLKARAINFFKGGSVVPNQALFDCTLQNYQIVD
                     QETRQAVEVTKRFVNSVLLGNPSHLVLTGKQGTGKSHLAMAAAWEVLKRSNYDKKILF
                     IGLQEMLDQIKFSYNNPELRKTIEGSLIADIKTADLVIIDDIGSELGKDASDSRAFGI
                     NTLNSFLDARQNLATIITTNLLGEELKKAYGTRTISRMFVNSDGFTMVFSQTADKRIK
                     PVKGAIA"
     gene            20148..20609
                     /locus_tag="U2K71_001903"
     CDS             20148..20609
                     /locus_tag="U2K71_001903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM7315957.1"
                     /translation="MPKILDACCGSRLFWFDKNNSDVTFMDCRQQYEELSTGHVINVD
                     PDVVADFRDMPFEDDVFDMVVFDPPHLRHVGENSWLAKKYGKLDELWPEDIRQGFAEC
                     MRVLRPSGTLIFKWNEEQIPLSDVLEAIGEQPLFGNKRSKTHWLVFMKKFS"
     gene            20635..21087
                     /locus_tag="U2K71_001904"
     CDS             20635..21087
                     /locus_tag="U2K71_001904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010756066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315958.1"
                     /translation="MKIVLNKCFGGFGLSHEAKMEIFKRKNIEVFPYINNFSYDSDDE
                     YTRYTGQKLGSMDFIYYFKKDPKIDKVTGIYSEIDRLYGIADDSSFSSDSNRGDKDLV
                     AVVEKLGSEASGPYASLKVVDIPDGAEWEISDYDGVETAHYGFQTGSI"
     gene            21193..21387
                     /locus_tag="U2K71_001905"
     CDS             21193..21387
                     /locus_tag="U2K71_001905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315959.1"
                     /translation="MDASEVVNKINSLKEVFGDVEVVINAQGYDNEFFKQITDISIQQ
                     GMEDEDGNFIDEVAILVTCE"
     gene            21418..21711
                     /locus_tag="U2K71_001906"
     CDS             21418..21711
                     /locus_tag="U2K71_001906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315960.1"
                     /translation="MKLKDGFYSSGHGIGGLMLDMPTKNPKTRKKPKFKVGDMVRCEA
                     EGFIYPFRGYVEHVYNHSAIIRIENTMECDKWLAKSKENLAVVRLVDMEVIKS"
     gene            21741..22181
                     /locus_tag="U2K71_001907"
     CDS             21741..22181
                     /locus_tag="U2K71_001907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM7315961.1"
                     /translation="MSVIPQLEGEEAIVNILENIDIKQTRKNARRLLKRYRNLERLVG
                     PVKIDFSVMTVTKNLKFTIDSQNEEIIEAISTRDSVIEALTRLSRIHFQVLYYSYCFP
                     NKMSMYQIGEKLGYSDRTIERMKAVALVEFAEAYKSGELISRTK"
     gene            22256..>22382
                     /locus_tag="U2K71_001908"
     CDS             22256..>22382
                     /locus_tag="U2K71_001908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEM7315962.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQ"
BASE COUNT         7091 a         3748 c         4693 g         6850 t
ORIGIN      
        1 aagttatcca tggaggttta gctcagctgg gagagcatct gccttacaag cagagggtca
       61 gcggttcgat cccgttaacc tccatatgag ccgttagctc agttggtaga gcatctgact
      121 tttaatcaga gggtcacagg ttcgagccct gtacggctca tatttttgcg ggtgtggcgg
      181 aattggcaga cgcactagat ttaggatcta gcgccttacg gcgtgggggt tcaagtccct
      241 tcacccgcat aatttaagtt ttacatgccg gcttagctca gttggtagag catctgattt
      301 gtaatcagag ggtcgagggt tcaagtcctt tagccggcat ctttaaacac gcggaaatag
      361 ctcagtggta gagcaccacc ttgccaaggt gggggtcgcg ggttcgaacc ccgttttccg
      421 ctttgccaaa gcaatcgcgc cggggtggcg gaactggcag acgcacagga cttaaaatcc
      481 tgcggtgagt gatcaccgta ccggttcgat tccggtcctc ggcatttcaa atatgcgccc
      541 atagctcaac tggatagagt gtttgactac ggatcaaaag gttaggggtt cgactcctct
      601 tgggcgcgtt aattaaacgg gaagtagctc agcttggtag agcacttggt ttgggaccaa
      661 ggggtcgcag gttcgaatcc tgtcttcccg ataattataa atttaaacac ggggccttag
      721 ctcagctggg agagcgcctg ctttgcacgc aggaggtcag cggttcgatc ccgctaggct
      781 ccatttcatt taaataatga atatcttttt cgcggtgtag ctcagctggc tagagcgtcc
      841 ggttcatacc cgggaggtcg ggggttcgat cccctccgcc gcgattttta agagcctgga
      901 cctttagctc agttggttag agcagacggc tcataaccgt ccggtcgtag gttcgagtcc
      961 tacaaggtcc attgtggcgt tgtaagtttc atatggagga ttacccaagt ccggctgaag
     1021 ggaacggtct tgaaaaccgt caggcgggta aaaccgtgca agggttcgaa tcccttatcc
     1081 tcctttttca tattatcgcg ggatggagca gtctggtagc tcgtcgggct cataacccga
     1141 aggtcgtagg ttcaaatcct gctcccgcaa ttaaaaacta aaaacactgg ttccgtggtg
     1201 taggggttaa catgcctgcc tgtcacgcag gagatcgcgg gttcgattcc cgtcggaacc
     1261 gccatgcggg tgtagtttag tggtaaaacc acagccttcc aagctgttgt cgcgagttcg
     1321 attctcgtca cccgctttta gttttttatg ggcctatagc tcagctggtt agagcgcacg
     1381 cctgataagc gtgaggtcga tggttcgagt ccatttaggc ccattagtac aaaatcttat
     1441 tggttttggg gaagtactca agtggctgaa gaggcgcccc tgctaagggt gtaggtcggg
     1501 aaaccggcgc gagggttcaa atccctcctt ctccgtttca atattttgtt ttacctggcc
     1561 cgttggtcaa gcggttaaga caccgccctt tcacggcggt aacacgggtt cgaatcccgt
     1621 acgggtcatt aatttaatat agttttacat ggaggattac ccaagtccgg ctgaagggaa
     1681 cggtcttgaa aaccgtcagg cgggtaaaac cgtgcaaggg ttcgaatccc ttatcctcct
     1741 ttttcatatt atcgcgggat ggagcagtct ggtagctcgt cgggctcata acccgaaggt
     1801 cgtaggttca aatcctgctc ccgcaattaa aaactaaaaa cattggttcc gtggtgtagg
     1861 ggttaacatg cctgcctgtc acgcaggaga tcgcgggttc gattcccgtc ggaaccgttt
     1921 ggctcggtag ctcagttggt agagcaatgg attgaagctc catgtgtcgg cagttcgatt
     1981 ctgtcccgcg ccattatgcg ggtgtagttt agtggtaaaa ccacagcctt ccaagctgtt
     2041 gtcgcgagtt cgattctcgt cacccgcttt tagtttttat gggcctatag ctcagctggt
     2101 tagagcgcac gcctgataag cgtgaggtcg atggttcgag tccatttagg cccatttatt
     2161 ttattggccc gttggtcaag cggttaagac accgcccttt cacggcggta acacgggttc
     2221 gaatcccgta cgggtcatgg aagatacttt ttgagtatct ttttcttttt atctgaaaga
     2281 atgattttaa aaagcttagc agagttatct ttatggatga ttgtgctaag cttttttgga
     2341 cagttctaat gtaaaatatt tttatatttt ttaagcaaaa aaatgaaaaa atacgagcag
     2401 aaaactgttt tatctgctat acttggttcg ttgcaagttc ccgaaaggat ccgcaggacg
     2461 gagatgcctt gtaaccgaat ttaactaggg ggaatcatta taatggtaga aagacactta
     2521 tttacatcag aatctgtttc tgaaggacat ccagataaaa ttgctgacca aatcagtgat
     2581 gcaatcttgg atgcaatttt aaaacaagat ccaacagcac gagtagcgtg tgaaacgtct
     2641 gtaacaactg gtcttgtttt agtatttgga gaaatatcta cgacggcata tgtagatatt
     2701 caaaaagtcg tacgggaaac aataaaagaa attggttata cacgtgcaaa attcggattt
     2761 gatggagata cagcagctgt attggttgcg attgatgaac aatctcctga tattgctcaa
     2821 ggagttgatg aagcacttga gatccgggat gaagataaaa aagacgtatt agatgagatt
     2881 ggtgctggtg accaaggttt aatgtttggg tttgctgtag atgaaacacc agaattgatg
     2941 cctttaccaa tcgctttgag tcatcgtctg gtacgacgct tggcagattt gcgtaaatca
     3001 aatgaattaa cgtatttacg tccagatgca aaatctcaag taacggttga atatgatgat
     3061 caaggacaac cggaacgcgt agatacaatc gttatttcaa cacagcatga tgatgcagta
     3121 gataatgaaa caattcgtca tgatgtcatc gaaaaagtag tgaaggaagt tattccagct
     3181 gaattattag atgatcaaac gaaatattat atcaatccga ctggccgatt tgtcattggt
     3241 ggtcctcaag gggatgccgg attaacagga agaaaaatca ttgttgatac gtatggcggt
     3301 tatgctcgtc atggtggcgg tgctttttct ggtaaagatg cgacaaaagt tgaccgttct
     3361 gccagctatg ctgcacgtta tattgctaaa aacattgttg cagcagggct tgctcgcaaa
     3421 gcagaagtac agctagctta tgcgattggt gttgctcaac ctgtttcgat ctcaatcaat
     3481 actttcggta ctggaacggt tccggaagaa gaactgattg ctgcagtgag agaaaacttc
     3541 gatcttcggc cagcaggaat catcgagatg cttgatttgc gtcgtccgat ttacaaacaa
     3601 acagctgctt acggtcattt tggtcgtaca gatgtagatt tgccttggga acatacggac
     3661 aaagtagatg cgttgaaagc aagtttagca aaataagtta tattcagctg gaacgatcgc
     3721 caagtggtgg ttgttccagc tttttttatg gcagttaacc tcttgaaaaa gatagtaatt
     3781 atttatctat ttttagatcc tgtcataatg aaagggagga ataagatgaa aaaagaaaca
     3841 aatgttactt tggtcacagt gagtattttc attgcaacgt tcatgacggc tattgaaggt
     3901 acaatcgtaa caaccgctat gccaacgatt gtcggttcat tacatggaat ggaaatcatg
     3961 aactgggtat tttcgattta tttgctgaca aacgctatgc ttactccgat ttacggaaaa
     4021 cttgcggata aagtgggacg gaaaccaatc tttatttttg ggacattatt gtttattatc
     4081 ggttcgttat tttgcggttt atctcaaagc atgattgggt tgattgctgc tcgtgcgatt
     4141 caagggatgg gtgctggggc aatgatgcca gtagctttga cgatcatcgg cgatctttat
     4201 tctattgaaa agcgagcaaa agttttaggt ttgaatagtt cagcctgggg gattgccagt
     4261 gtttttggac cgcttgcggg aggattcatc gttgaaacga tcagctggca ctggatcttt
     4321 tttatcaatg tgccgatcgg aattctatta attattttga tttggctata tttaaatgaa
     4381 ccaaaaatca tacatgagtc taaaccaatg gatatttttg gtagttttag tttgatggcc
     4441 atgcttgtct cattgctgtt agggttccaa ttattaggag acgaaggaat gagtccgctt
     4501 gtttctgctt tgctgctagg tagtatcgta atgttaggta tttttgtttt tgccgaaaaa
     4561 agagctaaag atcctgtcat catgatgcat ttattcaaaa atccactatt tgttgtggtc
     4621 aacttggctg cagcattggc tagtggtttt ttgatgggaa tcgatgttta tatccctatg
     4681 tggatgcaag gtgtattagg gctgaatgca gcaattggtg ggctggtatt agcaccatta
     4741 tcactagtct ggatggtcgg ttctttcctt gcaagcaagt ggatgactca aatgaaaact
     4801 cattgggtct tgagaattgg actactaatc gcagctattg ggggcgcttg gttgtatctt
     4861 atgcctttca gaagtaacta tttatggttt tttgctatat caacggtcat cggtataggc
     4921 ttaggtgtga ccatgactac ctcgactgtc agtgcacaaa atagtgtatc tcgtgaccag
     4981 cttggtgtag caacttcttt taatacgtta gttcgaacaa ttggtcaaac agtcatggtt
     5041 tctatttttg gtttattgat aaatggggta actaaacgag aacttgcagc agcaaattta
     5101 acaaatgatg cagatatcat gaataaactg gtgaatccac aaacggctaa atatattgat
     5161 gcggatattt tgttaccatt aagaaaaatt ctttatgatg ggttacattc cgtttatcta
     5221 gttggcttgt tactcgtgat cattgctttg gtcttaacgc ttttcgttca tgaaaaaagt
     5281 ggtaaaataa aacagtagtt tcggttaaga tatgtacgta gccgaatcat tcaaatttaa
     5341 agttattcaa aaataacttc ttactaagga aggcgaaaat atggcattta ttcaagcaaa
     5401 tatttattcg aatgtgttag aaatggaagt caatctaaat gtgatactac cgcaaacaac
     5461 aaaaaaggtg attggcacca gttcagagca agagacaacg gatatacctg ttttatattt
     5521 acttcatgga atgggtggca atcatagtgt atgggaaaga cgtacgtcga ttgaacgtta
     5581 tgtagcaaac tacggattag ctgtcatcat gccatctact gatcttggct ggtacacaga
     5641 tacagtttat gacatgaagt attggacatt cattgcagaa gagctacctg agatttgtca
     5701 tgaattattc ccgcaattaa caagaaaaag agaaaaaaca tatgccgctg gattatcgat
     5761 gggcggatat ggcgcgttaa aactaggcct tgcaaaatca gaaaatttcg cagcagtaat
     5821 ctctctttca ggcgctgttt ctgtaggaaa tcgaatggat gatcttttga tggttcgtaa
     5881 agcatctttt tgggaaggga tctttggacc attggatcaa atcgaaggtt caaaaaatga
     5941 tccgcaatac ttattgaaag aactagtaaa aagcggaaaa ccagttccaa gattttatat
     6001 ggcttgtggg gaatctgatt ttctttatca ggcaaatcag gaaatggtat ctgctatgca
     6061 ggaacaaggc ttggcggtca cctttgaaca aggacctggc gatcataact ggatcttctg
     6121 ggaccaatgg attcaacgtg cattggcgtg gctaatggaa gaaaaataaa aaaagagtct
     6181 taaaaagata gctcctatag agatcacaaa catagtgatc tctataggag cttttttact
     6241 gctcgttttc tcttctttta ttttgttggc ataagccttc aatataggtt tgtgcactcg
     6301 ttagaaagag tgctttttca ttcaattctg catgggactg catactttct ttaaatccct
     6361 ctaattgatt ttcaactaca taattacgca taaatagaac tgtttctttc aaaaattcat
     6421 ttccgtccat aattttttgt cgaagctgct gttcatgctg ctgatccatt aactgtccaa
     6481 tcaataaatt gtggagagag gcaatggcct gatgactttg ggtgactgaa aatccttcac
     6541 gaacaagtaa ttggatcatt tgctccaaat gttctaatcg actcggaaaa gcgggcacag
     6601 tattgatttc taattctgcc ccacaaggga aacgtgtata aaggtcatag taattttcca
     6661 tatactgcag caactgttca ttccaaggca aagttggatc tggaagcgct aattcttctt
     6721 ccattgtttc agccattttt tgcaagagct gttgtttgtt tttgaaatac caatataaag
     6781 caggtgcttg aacatgaatt ctgtttgcga tgtttcgcat ggaaagctgc gaaagagctt
     6841 gtgtttcagc caggatatca aaggcggcct gaatgatgat tgcttgagaa agctttattt
     6901 ccattttatc aacctcgtgt attgcattat ctttttaaac atggtaaatt gttagtcggt
     6961 ctaattttac agtgttaaat tagtcctgtc aatttaaaca aaggggtgta atcaacgaat
     7021 gaaattaatc ttacgacatg cgaaaaaata taaagtatct gtttttattt ccttgctgtc
     7081 cgtagcagtc atggttgcag cagcattatg gcaacctaag ttactacagc aagtattgga
     7141 agctattatt acagaagata atgatgaaat gaaaacaatt ggtatttacc tgatcagtct
     7201 ggctttgcta ggattagccg caggtgtaac gaacacgatt ttctctgcaa aagtagctca
     7261 aggcgtaagt gcagatatcc gagaagaagc atttcgaaag atccagacat tttcatttgg
     7321 aaatatcgaa cagttctctg ctggaaatct tgttgttcgt ctaacaaatg atatcacgca
     7381 aatccaaaat ttagtgatga tttctttgca atcacttttt cgaattccat tcttatttat
     7441 tggtgccttc attttagcta tgatcacgat gccgcaatta tggtggatca tcgtagcgct
     7501 tatcgttacg gtatttctga ttacggcact ttcatttaca aggatgggca agcacttcat
     7561 gatcattcaa aaactgattg atcgtgtgaa tagcattgca aaagaaaact tactagggat
     7621 tcgtgtagtg aaatcttttg tacaagaaga aaaccaattg aagtcattca gcaaagtaag
     7681 tgaggactta acaaaacaca acatcatagt cggtacatta ttttctgtga tgatcccgtc
     7741 cttcatgctg gctgccaatt tggctgtggt tggggcgatc ttttttgtga gcgatcttgt
     7801 taaagatgat cctacgttaa ttggtggaat tgcttcattc atgaactact tgatgcagat
     7861 tatgatggca atcattattg gcggaatgat gatgatgatg acttcaagag cagccgtatc
     7921 gctgaagcgg attgcggaaa ttttagatgc tgaaccagat cttacttatt tagacgttcc
     7981 agaacaagag ttgacgggtt ctgtcacttt tgaccatgtt tcttttcgtt atcctggtga
     8041 ggatacagat acgcttaaag atatcagctt ttctatcaaa cctggagaaa tggttggtat
     8101 cgtcggggcg actggtgccg ggaagtcaac actagcacaa ttgatccctc gtctatttga
     8161 tccgacagaa ggcaaagttg aagtaggtgg tgtagatctt cgtcaagtaa atgaaaagag
     8221 tttaagacaa acggtttcct ttgttttgca aaaagcaatt ttattctcag gaacgattgc
     8281 tcaaaacttg cgccaaggga aaaagaatgc aacagaaaaa gagatggaac atgcttcttc
     8341 tattgctcaa gcaaaagaat tcatcgaaaa gttatctgat cgttacgaag caccaataga
     8401 agaacgtagc agtaactttt ctggtggtca aaagcaacgt ttatccatta ctcgtggcgt
     8461 tatcggtaat ccaaagatct tgatcctaga tgacagtacg agtgcactgg atgctcgctc
     8521 tgaaaagctg gtccgagaag cactcgatcg agatttgaag ggaacaacga cgattgtcat
     8581 cgcacaaaaa attgcttctg ttgtcaaagc agaccgcatc ttagtattgg atgaaggcag
     8641 attagtcggc gaaggaaccc atgaggaatt ggtagctgga aaccgtgtct atcaggaaat
     8701 ctttgaaact caaaaaggaa cggaggagta agtatgactg atctaataaa agcaagtaaa
     8761 tttttctatc actatttgaa gcggtacaaa ctctcctttt tctttatctt tatcacagtg
     8821 atcattgcta cctatctaca agtaaaggca cctcaataca tcggggaagc ttttcaagaa
     8881 ttagctaatt acgtaggagg cttgatgcaa ggcgtagacg ataaaagcaa atttgtttct
     8941 gtaatatgga aactcttgct gttttatgta ctagctagtg cagccaactt tatctatagt
     9001 attttgttca cacaagtagt agggaagtca acgaaccgaa tgcgtatcgg tctattcaac
     9061 aaattggaaa aattgacgat ccgtttcttt gattctcatc aagacggtga aattttaagt
     9121 cgttttacca gtgacttaga taatattcaa aatagtttga accaagcttt gcttcaagtc
     9181 atcacgaatg ctgttttact tgtcgggatc ttgatcatga tgttccgcca aaatgtagag
     9241 ttagcttggg ctacaattgc ttccactcca gtagccgttt tgatagctgt cttgattatc
     9301 cgcaaagcac ggaaatatgt ggatcttcag caagatgaag tggggaaact aaatggttat
     9361 atggatgaaa aaatcagcgg acaacgtgtc atcatcacaa atggcttaca agaagaaacg
     9421 attgaaggat tcttagagca taatcaaagt gttcgcgaag caaccttcaa aggacaagct
     9481 tattctggtc tactatttcc gatgatgcaa ggaatgtcac tggtcaatac agcgatagtt
     9541 atctttttcg gcggctggtt agcattaaat ggtgatttgg aacgttcggt ggcattaggc
     9601 ttagttgtga cattcgtcca gtattcacaa caatattacc aaccactgat gcagatttca
     9661 tctggataca gcatgatcca attagcgatt acaggggcaa gacgattgaa tgaaatattt
     9721 gatgaaaaag atgaagtcaa accagaaaac ggtaaagcat ttgatggtgt tgaaaaaggt
     9781 cttgcgttgc agcacgtaga ttttggttat tctccagaaa aactagtctt gaaagatgtc
     9841 tcgatcaatg tggacaaagg agaaatggtt gctcttgttg gtccaacagg atcggggaaa
     9901 acgacgatca tgaatctgct aaaccgtttt tacgatgtca atgatggttc agtagtcatc
     9961 gatggaatag atatccgaga ttatgatctt gatgcattac gtagacatgt aggaatcgtt
    10021 ttgcaagaat ctgtattgtt ctccggaacg atcaaagaca atatcacatt cggcaaaccg
    10081 gacgcttcag atgaagaagt catcaatgca gcaaaacaag cgaatattca tgacttcatc
    10141 atgacgttag aaaaaggtta tgatacagag atatccgaag aaaataactt attcagtaca
    10201 ggtcaaaagc agttgatcag tattgctcga acgatcatca caaatccatc tttattgatt
    10261 ttggatgaag caacaagtaa tgtagacacg gtaacagaag caaggatcca aaaagcaatg
    10321 gatgaagcaa tcaaaggaag aaccagcttt gtgattgctc accggctgaa aacaatcttg
    10381 aatgcagata gaatcgttgt cttgcgtgat ggggaagtca ttgaagaagg cagtcatcat
    10441 gagttattag cagaaaatgg attttatgcg gaactttatc ataatcaatt tgtatttgaa
    10501 agttaaatta aaagaacctc gttataacgg ggttcttttt atatatttta tgttgtttgt
    10561 ttttataagg ggcagaaaag gggcaattta aatttctatt tttcctagtt tttctactag
    10621 ttgattttgc atatttttag ttgtatgagt ataaatcttt aatgttgttt ttgaatcttc
    10681 atgtcctaca cgatccataa tggcttttaa tgggattcct aattctgtaa gtagagaaat
    10741 gtgggaatgc cttaacatgt gagaagaaac ttttttatct aggtttaatt ctttggatac
    10801 tgctcttaaa gattgattat atgcttgaat agatatcggg ttaccatttt tagagatgaa
    10861 aatgtattta tctttattat tcttaattaa tttgttttct tcaattaatt gatctatgat
    10921 ttctttagca ttacttgata attcaatggt tctataagaa cctgtatttt tagttgttgt
    10981 tttaattttt aactcgtaat cataagtacc agaaaccgta attttattac catcataatc
    11041 agattcagtc aaagatatca attcaccata acgtagtcca gttaaaaaca aaaatttagt
    11101 aattaaagaa taacgtgact gttgatgtga acttttttgt ttactagcaa tcatttgaac
    11161 ttcttctttt gataaataac catctttttc tttttttatt tcttcgtttg tcttaggttt
    11221 tcttgctaaa gaaaccccat ttacgggatt atattttaat ccatatcgac ggttggcgaa
    11281 tttaaaaatc attcctgtaa aagtttttaa atgtattaca taattatatg ataaattttt
    11341 ttcaaaatag tagtaatcta gcatatcttg tataagatta ctattaacgt tttttacaag
    11401 tacatcatct tttatatatt ttgaaattaa tgatagataa acctgagttg ttcgatagga
    11461 actttctttt acagttggct tgtatttttc atcccattta gtttttaatt ctccaaaagt
    11521 tagtggtaat tcttcatttc gttttatttt ttctgcgatt ttttcattca aaagtaagtt
    11581 agcttgattc caagcttggc gtgaattgct agataaagta acggacactt ttctcttttt
    11641 ttcagttaag ggatccgtat aacgttcggt gtacttaaat tttttgtcat ttattttttc
    11701 taaccacatt atttctattc ctttcgaata tacgttccta ttaattgtta aaagaaaaag
    11761 cccgaaggct ttttatatat ttaatctttt gacaattcta aagatcgtct atattgttca
    11821 gctttagcta attttgtaaa tgttactgtt ccattataat tttctaaaac aactttctct
    11881 atttcgtcta gtgaaacttt gaaaaattct tttctttgat taattttatt aacttcttgt
    11941 tttctaaaat gattatgcaa tttagtttcc aaagacggag cgtcctctga aaaaatcatc
    12001 gcatgaacgt caaattcaaa tggaaccgat gcactactca attcttttat tctatccata
    12061 ggttctaatc ttctagtcat accaatttta tatatgtctt caccaaaaga cccaatatta
    12121 ctaattacat acacataacc ggctctagta tttaattctc tttgtaatac attttcacgt
    12181 ataacttcta attcttttaa tttttcttcc agttcttgaa ttttatttgc atataattct
    12241 ttttccacat ctgcattagc tttttgcata tatgcgatta atttggaaat ttcagaattg
    12301 aattgacgtt gttctttatc aatacgtttc ttttcacgat caatttctct acgaactttt
    12361 tcctcttcta tcatctgttc ttttattgct ttttgttgtt ctttttcttc ggtaattttc
    12421 actaaataag agtaatgaag ttctagttct tgaagtttaa actctaaaag acccttcctt
    12481 aattcagcat tgtcattctt gaataaatta ttaattttat caaatatttt tatgattttt
    12541 tccctagtcg agtctatatt agaaatagtt agtttattga gtaatccttt cacttctgaa
    12601 tcaaatggaa tgagcatttt tttctttaaa ttatttttct ctcgtttatt taaaataaga
    12661 gggacagaaa cagcatcgtc aataatatat tccttttctt ttaattttaa caaagaaagc
    12721 ttatttttta tttcagaaga tgaagtttct tctatatttg aaacgataag tgaagaatag
    12781 acatttttta atgtttcatc atacttttga tttatttcat catttaaatc ttctttctct
    12841 ttttttaata aaataatatc attttttaac atagtaattt gtttatttaa atctgatagt
    12901 gtttcttcct tacttaatat aaatctttct ctatcatgta atttatttag tttttcatca
    12961 tttttttgtt ctaattcata agcttctttt tttattgtgt tagctttcga cagtttagtg
    13021 attcctataa ttaataaaat aagtccaatc aataataaca ttcaaaactc ctcatttctt
    13081 tgatataatt attgtagagt ttactcagaa atgaggaaag agtccatgtg aaagcatggg
    13141 cttttttatt tttaatgttg taatactaaa tgttcaagat tatgaggaag ccctaaatat
    13201 tcaataattt ggaactgact catatcagat aaatcttgtt catctattgt tctagttaac
    13261 atttcaattg caaacatatt agcttcagct tctataccat aaatcataca actaaggttg
    13321 tttgctctaa aagctgctgt gcaaatacct ttatggagtc taacgtgggc tatttcatgc
    13381 cataatacaa atcttttcat agaaggtgat aggttttgat taattaatat cacataacat
    13441 cttttattgt gtatcgtttg tcctaatgtt ttattcccta aatcagtttc aattaaatct
    13501 atatcagaat atttgattat tttatatgga ttaatcgcat cagatccaaa ctctacaaat
    13561 aatttttcta tttcttctaa tatccagtat ttgtttaaat gcataccaaa gcccacctat
    13621 tgacgatatt ttttaggagt gaattttttc tttgctttaa ctttggttaa ggcaagtgca
    13681 agttgaatgg catcgtataa tttttcctta tcttcatcgt ctaaaggttc accataataa
    13741 tttgtttcag catctgaatt caagccttca agtaattttt cagcttgtaa agcaatatct
    13801 tgtttatctt tttctgttaa atcataataa tggcgtttat cagtacgtcc taaaagataa
    13861 tcagttgata cattaaagta atctgcaact ttttgtattt tatctccaga tggagtactt
    13921 ttattccatc tagacatgct tccatttccg aaatttaact ttctttcaag ttctgccaac
    13981 gtcattgaac gttcttttaa taagattttt atacggtcta atagcatagg atatgcattc
    14041 ctttcaaaat accgacaaaa aaagtatttt tctgcaaatt atctatttac aaatagagaa
    14101 tttgcagtta tactagtttt gtaagcaaga tatttacggc atacaaagac taaaagcaat
    14161 aaactaatag tctccccaga catcagtaag ctcgcttttt gtgcgctgta ttttgtatgc
    14221 ttacataata gcatatttgc agtttttgtc aaacttatta gacaaaaata ttagaaaata
    14281 ttcgataagg gggtatgaag catgacttta aaacagcaag tattaattgc tttagtaaag
    14341 aaaggttgga gtcaaagaga gttagctcga cgaatgggta tatcaattac ttatttacga
    14401 gatatcttca tcgaaaaaag aaaacctaag gaacgtttaa aacaaatcga agaacttttg
    14461 gatatcaagt tagaagtaga ttctaatgat cagccatcag aacaggaggc gtaaaaatgg
    14521 tacaacgttt gagcaaagcg gagtttaaag agaaatataa gtattctgaa agtacttatc
    14581 aaagaagaat gaaggaattt aaaaaatcac gttttagtga ggggtataac gcagttactt
    14641 ccaaagaaat aacaattgat gtcgatttat atgaacagtt tagaacatgg cgttctaaaa
    14701 atcgatttag agttagagct gtttgttaat agctaaaaaa tctaaattca ccacttctga
    14761 aaagaggagt tgctgctctc ctttcaagtt tgccaaagtc cacgtttttt cattttggca
    14821 actcctcttc ttggaggtgg tgacggcgag agacgaagga gggatgacat gaaggaagaa
    14881 tcaaaaatag ggattttgaa tgagaacaat tacacaagat taattattga aacagaagaa
    14941 gggaaaaaag tagcagaagt tacattgacc gatgcaactc ctgctgacgg ttatcgtatt
    15001 cgattaactc ctaaatatga ttaacctttt ggtgggtgtg gatcgtttcc gtgactatca
    15061 cggctttgaa tttttccatc tctgccatga ataaatagct cagatccttg atttttagaa
    15121 atctctctag cgatttttac tgcttctgat tttgtattag tatgtgcagt cgctttagaa
    15181 tttccagcac ctttgacatt ccaaccacca ttggaattag gtacaacatg ttgatttttt
    15241 cccatagttt tcacctccat ttctatagaa atattttatc aaatagaaat ggagaaattc
    15301 aataaacgaa aggagaagtg atatggcata taccttgcaa caagaacatc aaattctcgg
    15361 tttgattaaa caacgcagga aacaattaca agatgaccgt gcagcgctta gaaaagccga
    15421 tgagctatca gataggcaag ctgaactaat tgcttctgaa cttgaggatt tgagaatgct
    15481 agaaataaaa aatagggaga ttagattatg aagaagacag acacactttt tataggattc
    15541 attttggggt tattagtgat tgtagcgcac caaagtatta tcgggggaag cttgttcgca
    15601 gcattgatgg ttttaatcaa tctgcttgat tcaaaagaaa ggagcaacta tggcacgaga
    15661 agaagcgcta aaaatcggta aagtgattgc tgataatcgg tggggaaata gccgccctat
    15721 tattttaagc aagcaacata tcgaaaagca aaaagcatgg caacaaataa aaaagtgact
    15781 ctgccagcca gcaaagagtc acatacaaat ttataaaact aggagaatta tatcacatga
    15841 ataagaaaat tgaaaatctt atcgaagaat tgaaacgtga atgccaaaag caaggtgttt
    15901 ctattatttg tacagcccaa aaagaagggg aacttaaaag tctcgtttat ggtgagacaa
    15961 cagaaatttt actttgtctt gctatgcaag aagaacattt agatgaaaat ctcccattgt
    16021 cagcacatat tatgcgtaga attgctgtgg atgcttacga acaagcgaaa aatgaagaag
    16081 aaaatcaacc ttcaaatcat acttttgtta ttaataacaa ggaagattta gctgatgtga
    16141 tgactcgtat tctcaagggg gaatttcaat gatgaatgaa aaaacaattg atctactcat
    16201 tattttattt ttcacgttag tgattgtagc gaaaagttat gtaatctttg gcttgttatt
    16261 cgcaattacc atgcttgtgc tttgcgtgtt agcaagcaga aaagaggtta aacatgaacg
    16321 agttagcaaa tcttgacaac tatttaactg atcctgatta tgctaaacca ccatatgaag
    16381 caccaattga tgaggaggat gaagatgagt aaatctacct tagaaatgag ccatcaagaa
    16441 tggcttgaag accgtaagaa aggcatcgga ggttcggatg ttggaacaat tttaggattg
    16501 aataaatgga aatctcctta tcaactatgg ctagaaaaaa caggacaagt agtacttgaa
    16561 gaatcaggaa gtgagcccgc ttattggggt aatgttttag aagaagtggt tgctaaagag
    16621 tttcaggaac gtacaggcaa aaaagtacgc agaagaaacc aagtatttga acatccatta
    16681 catccatttt taagagcaaa tattgatcgg gaagtagtgg gagaaaatgc cattctggaa
    16741 tgcaaaacag ccaatcaatt tctcggtaaa gaatgggaag gcgaagaggt accgcttagc
    16801 tatctctgtc aagttcaaca ttacatgaac gttctaaaca aagactattg ttacatcgct
    16861 gtcttaatcg gtggtcaaaa atttatctgg aagcggattg aacgagatca agagctgatc
    16921 gatacaatca ctgaacaatt agtagagttt tgggaaacaa acgttcttgg aggtatcgag
    16981 ccaattattg atggtagtca agcgacagct gatttcttga aagagaagta tgcagatgtt
    17041 gaagacgttc aaacgacgtt accaattcat ttcgatgaac tagttgaaca gaaaaacgaa
    17101 ctcaaacgga ctaagaaaga aattgaatca gctatccgtc aagtagacaa cgagattatc
    17161 agcgagctag gtaaacgtga agccagtatc ggtatcactc aaaaaaacat catcagttgg
    17221 aaatttgtcc gtacgagacg tatgaactcg aagaaactag cagagaaata tccagatgtc
    17281 gcaaatgatg aagagattta taacgttact gaatcaagaa ggctaaccga aagggagatc
    17341 aaataatatg gcaacaaatg aatcgttaaa aaatcaattg gcagcaaagc cacagaaaca
    17401 agttgcacca ggtcaattag gacttaaagc tttgatgaat acaccaacaa tgagaaagaa
    17461 gttcgaagag gtgcttcatg acaatgctaa tgcttttatg tcgaatgtta tgactcttgt
    17521 atctaatgac agttatcttg cagatagtga accgatgtct atcatgagtg gtgcgttaac
    17581 tgctgcaaca ttaaatcttg ggctagataa gaatttaggt tatgcatatt tagttccatt
    17641 caatagtaaa aacaagcaaa caggaaaatg ggaaaagaaa gctcaattca tgcttggcta
    17701 taaaggatat atccaattag cccaacgatc aggtaaatac aaagcattaa atgtgatcga
    17761 agtttacgaa ggagaactaa aaagctggaa ccgactgaca gaagagtttg agtttgatcc
    17821 aaatggtaga acgtctgatg aagtcattgg atatgttggc tatttcgagt tactgaatgg
    17881 attcaaaaaa actgtctatt ggaccaaaca agaaatcgaa gctcatcgga ttgctaacaa
    17941 taaagatcgc gataagacaa agttaagtgg tgtgtgggca tctgattaca atgcaatggc
    18001 acgaaaaact gttttgagaa atcttctttc taaatgggga atcttgtcca tcgaaatgca
    18061 agaagccacc acatcggatg agagagtcca aagggttcaa gaagacggca gcattattgc
    18121 tgaaacagaa gttgaagaag atattcctga aagaaaagaa gcagaggtta tatctgaaga
    18181 aaatgaagat gtacaaactg gattatttga tgcatctaat ccgccgttaa acaaataatg
    18241 agggagcttt ctcccttgct tttctagagg agaaatacga atgaacacag gatatgtcaa
    18301 attacatcga aaagtgatga attcattcgt ttggaccaat ccttatatgt ataaattatg
    18361 gaacttgtgt ttgatgaaag cgagtcatga gaatcgcaag atacttttta atggaaaaga
    18421 gatacaagtg aacagcggag aattcgtcac agggcgcgat gctatcacat ctgagatgaa
    18481 cgaaggcgtg aaacgtgaac atcaagtgaa cagcggttct gtatggagat ggctaaaaca
    18541 atttgaaaaa aacggaatgt tgaacatcaa atcaactacg aaatacagcg ttatatcaat
    18601 aaaaaactgg tctttgtacc aaagcagtga acaacaaatg aacatcaaac gcacaacaag
    18661 tgaacaacaa gtgcacacaa acaagaatga aaagaattat aagaatgaaa agaataataa
    18721 aacatcatcg tcgcaaccac gcaaaaagcg tgtttacgac accgactcag tttactacat
    18781 tctcgcggag gagttattca aacagatttg tcagaatcag gaaatcaaaa agccgaatct
    18841 gcaaagttgg gcagatgaca ttcggaaaat gatcgagatc gacaagcgga ccgaaaacca
    18901 ggtacgggga atgattgaat ggagtcagca caatgtgttt tgggcatcga atattttatc
    18961 tgctaaaaaa ttgcgagaaa aatacgacac aatggcagcg caagcaaatc gtgattataa
    19021 aacaaaacaa actaaaacgc ttgaatacga gaaatttagc acagatgagt tgcctatttg
    19081 agaggaggcg taggcatgga aactgttggg gaaatcatgg aaaagctgat acagaaagtg
    19141 cttgtccaac gtggcgaatg tcctgaatgc ggacagcctt tgtatggatg gcgtacgaag
    19201 aaccctgatg gctcagaacg ttgtaagcca acctgtatgc aatgtgggta taaagcattg
    19261 cgagttcagg aagacttaca aacagaacga atttacaacg agagcctgaa agcaagagca
    19321 atcaattttt tcaaaggtgg ttctgttgtg cctaatcaag cgttgtttga ttgcacattg
    19381 cagaattatc aaattgtcga tcaagaaaca agacaagcgg ttgaagtaac caaacgcttt
    19441 gttaattcag tcttgttagg aaatccaagt caccttgttt taactggaaa acaaggaaca
    19501 ggtaaaagtc acctagcgat ggcagcggct tgggaagtct tgaagcgatc aaactacgat
    19561 aaaaaaatct tatttatcgg gttacaggaa atgctggatc aaatcaaatt ttcgtacaac
    19621 aatcctgaac tcagaaaaac gattgaggga tcgttgattg cagatatcaa aacagcggat
    19681 ttagtcatca ttgatgatat cggttcagaa ctaggaaaag atgcatcaga tagtcgagcg
    19741 tttggcataa acacgctaaa ttcgttcttg gatgcacgac agaacctagc aacgattatc
    19801 acaacgaact tgcttggtga agaactgaaa aaagcttacg gtacgagaac aatatcaaga
    19861 atgtttgtca actctgatgg atttacgatg gtattttctc aaacagcaga caagcgcata
    19921 aaaccagtca aaggagcgat cgcatgaatt tgactaaaca agaaaaaaga atctgtcgtc
    19981 agataatgtt gcaaattata ggtgatcgtg acatggacga attatctcaa caagagagtc
    20041 gaatggttcg gaagataagt aagctaatta tttatcggga tacagaaaga tttctcactg
    20101 ttttaggtaa cgtctacgag aatccagagt tattggaggt agagtaattg ccaaaaatat
    20161 tagatgcatg ttgcggtagt agattgttct ggtttgataa gaacaattct gacgtaacat
    20221 ttatggattg tcgtcagcag tatgaagaat tatcaacagg tcatgtgatc aatgttgatc
    20281 cagatgtggt cgcagacttt cgcgatatgc cttttgaaga tgatgtgttc gatatggttg
    20341 tatttgaccc gccacactta agacatgtcg gcgaaaactc atggcttgcc aaaaaatatg
    20401 gaaaattaga cgaactttgg ccagaagaca tcaggcaagg ttttgcagag tgtatgcgag
    20461 ttttacgacc ttcaggaaca ttaattttta agtggaacga ggaacagata ccactttctg
    20521 atgtgttaga ggctattgga gaacagccat tgtttggtaa taaacgaagc aaaacacatt
    20581 ggcttgtgtt tatgaaaaaa ttcagctaac ggaggaaaca aaggaggaaa agtaatgaag
    20641 attgtattaa ataaatgttt tggtggcttt gggttgagcc atgaagcaaa aatggaaatt
    20701 tttaaaagaa aaaatattga agtattccca tacatcaata actttagcta tgactcagat
    20761 gatgaataca cgagatacac aggccaaaag ctaggttcga tggattttat ctactatttc
    20821 aaaaaagacc cgaaaatcga caaagttaca ggaatttata gcgaaattga ccgactgtac
    20881 ggaattgctg atgattccag tttttcaagt gattcaaaca gaggtgataa ggacctcgtt
    20941 gctgttgtcg aaaaactagg aagtgaggcc agtggaccat atgcaagtct taaagtcgtt
    21001 gatatacccg atggggctga gtgggaaata agtgattatg acggcgttga aactgcacat
    21061 tatggttttc aaacaggtag tatttagtcc actaatgaca actaaagagg aggcagaagg
    21121 atgagtgaag atgaatactt tgaacagtag tctattaacg acagagattt ttaactggaa
    21181 aggggtgtag tcatggacgc atcggaagta gtaaataaaa ttaatagtct caaagaggtt
    21241 ttcggagacg tcgaagtagt gataaacgca caaggctatg acaacgaatt ttttaagcag
    21301 attaccgaca ttagcattca gcaaggaatg gaagacgaag acggaaactt tatcgatgaa
    21361 gtagccattt tagtgacttg cgagtaagta attgacagct aaagaggagg aagcggaatg
    21421 aaactaaaag acggatttta ctccagcggc cacggcatcg gcggtttaat gctagatatg
    21481 ccgacaaaga atcctaaaac acgtaagaaa ccaaaattca aagtcggtga catggttcgc
    21541 tgcgaagcag aaggattcat ctatccattt cgtggatatg tagaacacgt ctataatcac
    21601 tcagcaatca ttcgcattga aaacacgatg gaatgtgata agtggttagc gaaaagcaaa
    21661 gagaatttag cagtggtgag attggtggac atggaagtta taaaatctta attttgcttt
    21721 tttacaacta tcctacaaca atgagcgtta ttccacaact ggaaggagaa gaggcaattg
    21781 tgaacatttt agagaatata gatattaagc aaacaaggaa aaatgctaga cgattactaa
    21841 aaagatacag aaatttagaa cgtttggtcg gaccagtgaa aatagacttt tccgtgatga
    21901 ctgttaccaa aaatttgaaa ttcacaattg acagtcaaaa cgaagaaatc attgaagcga
    21961 taagtactcg agattcggtt atcgaagcat taacacggct aagtagaatc catttccagg
    22021 tactttatta tagttattgt tttcctaata agatgtcaat gtatcaaata ggagaaaaat
    22081 taggatattc cgatagaact atcgagagaa tgaaggcagt agctttggtt gaatttgctg
    22141 aggcgtataa atcaggagaa ctcatttcac gtacaaaata aaaaaagtcg gattcctccg
    22201 actatgaata atatttccga cataagtatt atatcataat tgggggaatc agaggatggt
    22261 acttttcgat gtaaagaaat atgagacacc aagcgcgaaa gatgttgata tggaacgcac
    22321 aaaacataat gtcgctgtgt ttctttcagc atatctatca gctagatgta gagtagggca
    22381 ac
//
LOCUS       DASHHB010000043        21968 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.044, whole genome shotgun sequence.
ACCESSION   DASHHB010000043 DASHHB010000000
VERSION     DASHHB010000043.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21968)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21968)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21968
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.044"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            153..1859
                     /gene="efbA"
                     /locus_tag="U2K71_001909"
     CDS             153..1859
                     /gene="efbA"
                     /locus_tag="U2K71_001909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin-binding protein EfbA"
                     /protein_id="HEM7315963.1"
                     /translation="MSFDGVFTHAMVNELRETLLSGRISKIHQPYENEIVLVIRSRGK
                     NHRLLLSAHPSYARVQITQIDYQNPDNPPNFVMMLRKYLDGAILEDIEQIENDRVIHF
                     HFAKRNELGDLQNIILIVELMGRHSTIVLVNRETGKILDAIKHIGSSQNTYRSLLPGV
                     EYVAPPKQEVLNPFSSEKEKIFQRLSQTELDPKAIQRQFQGIGFDIAQELTKRLLERP
                     NEKMVVWDEFFSAISHQPIPTFYETENKDFFTPIAYQVLSEQASAVTAYPTLSQLLDS
                     YYHEKAEKDRAKQQGGELIRKIENELKRNKNKLKKREQTLKESENAENYRRNGELLTT
                     FLTQVPRGAKEVVLPNYYEEDRPIKIALDPALTPNQNAQKYFHRYQKLKNAVKLIGEQ
                     IQEAKDEIQYLESVLSQLEIAGPMDIEAIKEELTSEGYLKKKNLKKQKRKKPSQPDQY
                     FSSDGTLILVGKNNLQNDQLTMKTAKKTDYWLHAKNIPGSHVIIKSDKPSDETITEAA
                     ELAAYFSKYRYSAQVPVDLVQVKHIRKPNGAKPGYVIYENQKTIIVTPEEEKITAMKQ
                     NR"
     gene            complement(2017..2919)
                     /locus_tag="U2K71_001910"
     CDS             complement(2017..2919)
                     /locus_tag="U2K71_001910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM7315964.1"
                     /translation="MNIQQMKYVVAVANNGSFREAAKKMFVSQPSLSAGIKELETELG
                     ITLFTRTNRGAYLTEEGQDFLNRAEKILVQLDTLETHYLTSEKRDNFSIAAQHYDFLG
                     PLTAQLLEEFKDEIKQFRIMETTTARVIEEVKEQHSEIGIIYMNEHNQAGIRRYLQQG
                     ELSGRSIGKFKTHIFLRQGHPLAEHAEITKEELLSYPQVRFTQDGNNFPYFYEDLIEI
                     PDQETVIYTSDRGTLMNIVLETDAYASGSGIVIGGIREHLRLIPLAEGELNEFYIIYS
                     AKRPLSEIAQRFIKELTRLLDQQS"
     gene            complement(2916..3608)
                     /locus_tag="U2K71_001911"
     CDS             complement(2916..3608)
                     /locus_tag="U2K71_001911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase family protein"
                     /protein_id="HEM7315965.1"
                     /translation="MDVEWGYSGDKGPENWASLCDWFARGAEFPLQSPIHLTKGEETK
                     IEGDTLSFHYQKQRFTDKEFKNTIHLVPFDQLSYVEFKKERYYLTDIHFHLPSEHIIN
                     GKQEDIEFHFVHMNSKKENLVVGVMYQLMVQEGWLYNQENGESWNLEKHEHWVNPDVF
                     FPEQKSHFHYIGSLTTPPTSGPIQWFVMDHVGKLNQEFLLPFDGQYARPNNRPIQPLN
                     GREIYYFEEFEQ"
     gene            complement(3651..4292)
                     /locus_tag="U2K71_001912"
     CDS             complement(3651..4292)
                     /locus_tag="U2K71_001912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM7315966.1"
                     /translation="MGLNEFILGLNNLETITRAPGFFKYTEHTVAAHSYRVASIAQVL
                     GDIEEANGQPIDWKLLYEKSLNHDYTERFIGDIKTPVKYASKELRGMLQTVEEKMTDE
                     FINQEIPAEFQEVYRRRLFEGKDETVEGELLAISDKVDLLYESFDEIIKNNPEKVYKE
                     MFIEAVTTIKSFEHRPSVQYFFKEIFPELLNQEFYGKEAFLETVSTIVKKEEK"
     gene            complement(4330..4644)
                     /locus_tag="U2K71_001913"
     CDS             complement(4330..4644)
                     /locus_tag="U2K71_001913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315967.1"
                     /translation="MADLSALDEDREIRFVSPESSIGIAKIQKLIARSIFSSGMKRNF
                     YRDQFLIFLKFIFLRLVSRKKRIISSLFSIIYRFSLFFGKGAVSKMVKSGNVANISYL
                     MI"
     gene            complement(4632..5984)
                     /locus_tag="U2K71_001914"
                     /pseudo
     CDS             complement(4632..5984)
                     /locus_tag="U2K71_001914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372436.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
     gene            complement(5981..6913)
                     /locus_tag="U2K71_001915"
     CDS             complement(5981..6913)
                     /locus_tag="U2K71_001915"
                     /EC_number="1.3.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase"
                     /protein_id="HEM7315968.1"
                     /translation="MNENPLAVKLPGLELKNPIMPASGCFGFGKEYGKYYDLNQLGSI
                     MVKATTPNARFGNPTPRVAETPSGMLNAIGLQNPGLDVVMHKLLPELEEYNELPIIAN
                     VAGACEEDYVEVCSKIGEAPNVKAIELNISCPNVKHGGIAFGTDSDVAFQLTQAVKKV
                     SSVPVYVKLSPNVTDIVPIAQAIEAGGADGFTMINTLLGMRIDLKTRKPILANQTGGL
                     SGPAIKPVAIRLIHQVASISSLPIIGMGGVQTVDDVLEMFMAGASAVAIGTANFTDPY
                     ICPKLIKELPVRMSELGIESLERLIKEVREERER"
     gene            complement(6910..7686)
                     /locus_tag="U2K71_001916"
     CDS             complement(6910..7686)
                     /locus_tag="U2K71_001916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotate dehydrogenase electron transfer
                     subunit"
                     /protein_id="HEM7315969.1"
                     /translation="MKQEMMEIVQQKQLAPKIFEMTLKGDLVKEMQQPGQFLHILVPR
                     ADLLLRRPISLNHIDHETNTCRIIYRVEGEGTRVFSTLSAGDHLDVMGPLGNGFDLSN
                     LHTGDEVFIIGGGIGVPPLYELSKQLVEKGIKPTHFLGFATHEVVYYEKEFNRLGETR
                     IATDDGTYGMHGNVGNLLLGTKTEPTAVFACGSNGLLKTVEQLFSSHQNVQLSLESRM
                     ACGIGACYACVCHTPEDETGTKSVKVCDEGPIFKIGEVII"
     gene            complement(7740..10925)
                     /gene="carB"
                     /locus_tag="U2K71_001917"
     CDS             complement(7740..10925)
                     /gene="carB"
                     /locus_tag="U2K71_001917"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001126118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HEM7315970.1"
                     /translation="MPKRTDIKKIMVIGSGPIIIGQAAEFDYAGTQACLALKEEGYEV
                     VLVNSNPATIMTDKEVADQVYIEPITLEFVSRILRKERPDALLPTLGGQTGLNMAMEL
                     AASGILEELNIELLGTKLSAIDQAEDRDLFKQLMKELNQPIPESEIVNTVEEAVDFAN
                     KIGYPVIVRPAFTLGGTGGGMCENEEELRIVSENGLSLSPATQCLIERSIAGFKEIEY
                     EVMRDSADNAIVVCNMENFDPVGIHTGDSIVFAPSQTLSDHEYQMLRDASLSIIRALK
                     IEGGCNVQLALDPHSFNYYVIEVNPRVSRSSALASKATGYPIAKLAAKIALGLTLDEM
                     KNPVTGTTFAEFEPALDYVVAKIPRWPFDKFENGERLLGTQMKATGEVMAIGRNIEES
                     LLKAVRSLEIGTHHIELPELADITDDDLIQKVVRAQDDRLFYVAEAIRRGYTIEELAD
                     LTKIDLFFLDKLLHIVEIEQQLKQQPLDDKILKTAKQNGFTDKKVAELWEINEEAVRQ
                     MRKENDLIPVYKMVDTCAAEFESKTPYFYSSYEYENESSRSEKPSILVLGSGPIRIGQ
                     GVEFDYATVHSVKAIQQAGYEAIIMNSNPETVSTDFSISDKLYFEPLTFEDVMNVIEL
                     EQPIGVIVQFGGQTAINLAEPLTAAGVKILGTTIEDLDRAENRDLFEQALKELDIPQP
                     LGDTATSKEEAVVIASRIGYPVLVRPSYVLGGRAMEIVENQRDLEDYMEHAVKASPEH
                     PVLVDRYLLGGECEVDAICDGETVLIPGIMEHIERAGVHSGDSMAVYPPQTLSESIKK
                     TIEDYTIRLALGLNCVGMMNIQFVIHENEVYVIEVNPRASRTVPFLSKVTGIPMAQIA
                     TKAILGEKLKTLGYENGLYPETKEVHVKAPVFSFTKLQKVDTYLGPEMKSTGEVMGSD
                     RQLDKALYKAFEASGLHLPEYGAVLFTIADETKEEALELAKRFTEIGYSLIGTKHTAA
                     YFEKNGLVVETVAKISEEAAEKNVVDLIRDGKTQVVVNTIDKDRGNASKDGFIIRREA
                     VEHGIPLFTSLDTADAIVRVMESRAFSTNPI"
     gene            complement(10930..12006)
                     /gene="carA"
                     /locus_tag="U2K71_001918"
     CDS             complement(10930..12006)
                     /gene="carA"
                     /locus_tag="U2K71_001918"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing carbamoyl-phosphate
                     synthase small subunit"
                     /protein_id="HEM7315971.1"
                     /translation="MERLLILEDGTVFEGKAFGADASVFGEIVFTTSMTGYQETITDQ
                     SFNGQIITFTYPMVGNYGVNRDDYESISPTCKGVVVKEHARVASNWRNQMTLDSFLKQ
                     KGIPGISGIDTRALTRKIREVGTMKASIVDAGDSFEHAFDQLKATVLATNQVQQVSTN
                     KPYPSPGTGRKVIVIDFGLKHSILRELSKRNCNLTVLPYNTDAETILALSPDGVMLTN
                     GPGDPKDVPEAIEMIKKIQGKVPIFGICLGHQLFALANGADTYKMKFGHRGLNHPVRE
                     VATGRIDFTSQNHGYAVDEATIDPEKLIVTHVEVNDGTVEGVKHRDYPAFSVQFHPDA
                     APGPHDALHLFDEFMEMMDAGKEQ"
     gene            complement(12011..13291)
                     /locus_tag="U2K71_001919"
     CDS             complement(12011..13291)
                     /locus_tag="U2K71_001919"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HEM7315972.1"
                     /translation="MKTLIKNGQINTRKNMTTPAEIWIEDGRIKAIGTGFSEAEFDEV
                     FDAKGQLITPGLVDVHVHLREPGFTYKETIEAGTRSAARGGFTTVCAMPNLNPVPDTA
                     EKLRQVYDIIRKDAVVKVLQYAPITENLRSEKLVDQEALIEEGAFAFTNDGVGVQTAG
                     TMYLAMKEAAKNNKALVAHTEDESLLFGGVMHAGKKAEELGLPGILSVTESSQIARDL
                     LLAEATGVHYHVCHVSTKESVRVIRDAKKAGIHVTAEVSPHHLILIDEDIPEDFGFWK
                     MNPPLRGREDREALIEGLLDGTIDCIATDHAPHGLEEKSQSFMKSPFGIVGSETAFQL
                     IYTHFVETGRFTLEQVINWLAVKPAEIFGLNAGTLTVGAPADVAVFDITQTCTIDKED
                     FLSKGENTPFIGWKVKGETQMTFVNGKLVWQKGE"
     gene            complement(13310..14236)
                     /locus_tag="U2K71_001920"
     CDS             complement(13310..14236)
                     /locus_tag="U2K71_001920"
                     /EC_number="2.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate carbamoyltransferase catalytic
                     subunit"
                     /protein_id="HEM7315973.1"
                     /translation="MIIKSERISLKHLLTVEALTDQEVMGLIRRGQEFKQGANWTPQK
                     RQYFATNLFFENSTRTHKSFDVAEKKLGLEVIEFEASTSSVQKGETLYDTVLTMSALG
                     VDVAVIRHGDENYYDELIQSKTIQCSIINGGDGSGQHPTQCLLDLMTIYEEFGHFEGL
                     KVAIVGDITHSRVAKSNMQMLKRLGAQVFFSGPREWYDDEFEVYGHYMPLDELLDQVD
                     VMMMLRVQHERHDGKESFSKEGYHQEHGLTVERAKKLQKHAIIMHPAPVNRDVELADS
                     LVEGLQSRIVQQMSNGVFVRMAILEAVLNGKA"
     gene            complement(14249..15532)
                     /locus_tag="U2K71_001921"
     CDS             complement(14249..15532)
                     /locus_tag="U2K71_001921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil permease"
                     /protein_id="HEM7315974.1"
                     /translation="MSEKKGFHNDDVVLDIHDRPTPGHWVGLSLQHLFTMFGATVLVP
                     ILVGIDPGIALVSSGLGTLVYLFTTKGKIPAYLGSSFAFIAAMQLLMKTDGYPAIAQG
                     AVTTGLVYLIVALIIKKIGSAWLDKILPPIVVGPVIMVIGLGLAANAATNAMYNQGTY
                     DFKYIAVALITLGLTIFFNMWLRGFLGLIPILLGIISGYIVALLFGIVDTKPIVDAAW
                     FAVPNFEIPFVQYEPKLYLGAITTMAPIAFVTMTEHIGHLMVLNKLTKRNFFEEPGLH
                     KTLTGDGLAQIVAAFVGGPPVTSYGENIGVLAITRVHSVFVIGGAAVFAVILGFVGKL
                     SALILSIPGPVISGISFILFGVIAASGMKILIENKIDFDKKKNLLIASVILVVGIGGL
                     VFEVGTFTLSAMALATVLGIILNLILPETSRSEEN"
     gene            complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="U2K71_001922"
     CDS             complement(15546..16085)
                     /gene="pyrR"
                     /locus_tag="U2K71_001922"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional pyr operon transcriptional
                     regulator/uracil phosphoribosyltransferase PyrR"
                     /protein_id="HEM7315975.1"
                     /translation="MQAKEVVDQVTMKRALTRITYEIIERNHSIQDIVLVGIKTRGIY
                     IASRIAERLKQLEDIDIPVGELDITLYRDDKKENPEEPELHSSDIPVSLEGKEVILID
                     DVLYTGRTIRAAMDAVMDFGRPRKISLAVLVDRGHRELPIRADYVGKNIPTSRAEEIL
                     VEMQELDGQDRIMILKEED"
     gene            complement(16320..17231)
                     /locus_tag="U2K71_001923"
     CDS             complement(16320..17231)
                     /locus_tag="U2K71_001923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HEM7315976.1"
                     /translation="MTELKTTIHHESGRIDKVLTNLFTDYSRSQIQLWLKNGAVSVNG
                     QPVKANYKVKQGDEITVLIPEPETLDVVPENIPLTIVYEDEDVAVINKPQGMVVHPSA
                     GHTHGTMVNALMYHIKDLSTINGVIRPGIVHRIDKDTSGLLMVAKNDQAHESLAQQLK
                     DKTSLRKYVALVHGEIPHEKGTINAPIGRSKENRKMQAIREDGKSAVTHFTVLERFEG
                     FTLVELKLETGRTHQIRVHMKYIGYPLAGDPVYGPKKTLKGNGQFLHAQMLGFTHPTT
                     NEKMIFEAPLPEVFEKTLEKLRKNIAF"
     gene            complement(17228..17692)
                     /locus_tag="U2K71_001924"
     CDS             complement(17228..17692)
                     /locus_tag="U2K71_001924"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HEM7315977.1"
                     /translation="MLIIYWIFSALLVGVDQLVKWWITDNLALGETKNLIPGILSLNH
                     IRNTGAAWSMLEGKMWFFTIVTLIAVVVILTLMIKNREKGNRWFMIGLSLILAGAIGN
                     FIDRIRLGYVVDMFQTDFMSFPIFNVADVTLVCGVICMLIYIILDEKDQRKK"
     gene            complement(17697..18395)
                     /locus_tag="U2K71_001925"
     CDS             complement(17697..18395)
                     /locus_tag="U2K71_001925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HEM7315978.1"
                     /translation="MANSDLFLISFNRIEKWMRDEMGNARNMGFTELVRRLAQRKQLM
                     IRKYEDDLLQLAQLRNAIVHDRIAVDFVIAEPNDWTVKRIQLIEQELIQPETVLPRFA
                     KRVTGFEQDLPLLELLKIVAEKRYSQFPLYNKGKFVALITLRNIGFWLAKESQKGPVD
                     LTNKRALDLIIQNGKYTNYHFVPAKTHIYEVEAMFREQGTLEAVLITKDGNPDGNLLG
                     IVRPRDIYKQIEKD"
     gene            complement(18382..18768)
                     /locus_tag="U2K71_001926"
     CDS             complement(18382..18768)
                     /locus_tag="U2K71_001926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315979.1"
                     /translation="MKTTFIRKTGFYGMGSSIQLRINNEKRSVLSHEQKITLDLEVPF
                     TMQVTFYWLKSPVYTVKEAKPAYVITMNYLILQIYPFLFLATGLGAVFLQNLFFSFFA
                     VIVLIAFFFYIKNRAYLIKEENYGKL"
     gene            complement(18915..20585)
                     /locus_tag="U2K71_001927"
     CDS             complement(18915..20585)
                     /locus_tag="U2K71_001927"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HEM7315980.1"
                     /translation="MKNDLQISQETELEPIVEVASKLDLKEDDLELYGKYKAKINFSG
                     INRSKDNAEGHLILVTSINPTPAGEGKSTITVGLGDALNKINKKSVIALREPSLGPVM
                     GIKGGAAGGGYAQVLPMEDINLHFTGDMHAITTANNALSALLDNHIHQGNELNIDARR
                     VIWKRVVDLNDRELRKVIVGLGGPIQGVPREDGFDITVASEIMAILCLAADLEDLKAR
                     LARIVVGYTFDRQPVTAGDLKAEGALALLLKDAIKPNLVQTIYGTPAFVHGGPFANIA
                     HGCNSILATKTALRLADYVVTEAGFGADLGGEKFLDIKVPNLKKAPDAVVIVATVRAL
                     KMHGGMKKDELKNENLDALKVGFANLKRHIRNMEQYQLPVIVAINEFVTDTDNELTLL
                     EHLCENQGILAKRASVWADGAEGGVDLAKAVVRLIDRKEANYKPLYRLEETIQEKTET
                     IVKKIYGGNGVVFSKKAQKQIEEFTKNGWDKLPICMAKTQYSFSDDPSLLGAPEDFTI
                     TIREIIPKLGAGFLVALTGDVMTMPGLPKKPAALNMDVTNDGEVLGLF"
     gene            20768..21001
                     /locus_tag="U2K71_001928"
     CDS             20768..21001
                     /locus_tag="U2K71_001928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HEM7315981.1"
                     /translation="MTTGIVKWFDNKKGYGFISYDDTEEIFVHFTAIEEEGFKTLEEN
                     QVVEFEIIEGNRGTQAAHVKKGNVQTTDTSMHE"
     gene            complement(21100..21531)
                     /locus_tag="U2K71_001929"
     CDS             complement(21100..21531)
                     /locus_tag="U2K71_001929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EbsA family protein"
                     /protein_id="HEM7315982.1"
                     /translation="MKKNIRWQPELAHTIIYWSLTLIILFFSLILSLENTGPYWKSNF
                     VLGIFLVFLFLGLRRSFILKSNSVIIRYAAFWKDREISVGQIRSLTPTNTGVQMMLQD
                     EKAPSVFMMRKKDQVVLFDHFEKNGPKSESIPYPYTKKRKS"
     gene            21610..>21968
                     /locus_tag="U2K71_001930"
     CDS             21610..>21968
                     /locus_tag="U2K71_001930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI family protein"
                     /protein_id="HEM7315983.1"
                     /translation="MLKVYIDASTKGNPGPSGGGILIVYQGKQEQLSIPLSIGTNHQA
                     EFEVFLKTLEFLKKNDLQNETIFCFSDSKTLVSTVDKNATKNELFLPYLQRIQELLSE
                     FSLLILQWLPENKNKGA"
BASE COUNT         6205 a         4911 c         3883 g         6969 t
ORIGIN      
        1 taaaaatttg gatttcaacg atagaaacac tgttttttat gtaattagtc caagattttt
       61 ctagactttt gctttactat tcggccaaat tggtttttct tctttttttc gttataatga
      121 atatacttat gaaaagaaat gaggaatgaa ccatgtcctt tgatggtgta tttacccacg
      181 cgatggtcaa cgaattgcgt gaaacgttac tatctggccg gatctcgaaa atccatcagc
      241 cttatgaaaa tgaaatcgtg ctggtgattc gttcacgcgg caaaaaccat cgattattat
      301 tatctgcgca cccaagttat gctcgtgtac agattaccca aatcgattat cagaatcctg
      361 ataatccgcc taacttcgtg atgatgcttc gaaaatattt ggatggcgct attttagaag
      421 acatcgaaca aatcgaaaat gatcgagtga tccatttcca ctttgctaaa cgaaacgagt
      481 tgggtgatct acaaaatatc atattgatcg ttgaattaat gggacgtcat agtacgatcg
      541 tcttagttaa tcgagaaact ggaaaaatac tggatgcgat caaacacatt ggaagctctc
      601 aaaacactta tcgttctttg cttcctggtg tagaatatgt tgctccgcca aaacaagagg
      661 tcttgaaccc atttagctcc gagaaagaaa aaatctttca gcggctttcc caaactgaac
      721 tagatccaaa agcaatccaa cgtcagtttc agggaatcgg tttcgatatc gctcaagagc
      781 tgaccaaaag attactggaa cgtcctaatg aaaagatggt tgtatgggac gaatttttct
      841 cagccatcag ccatcagcct attcctactt tttacgaaac ggaaaataaa gattttttca
      901 caccgattgc ttatcaggta ttgtccgaac aggcatctgc tgtaacagct tatccaacac
      961 tcagtcaatt attagatagc tattatcacg aaaaagcaga gaaagatcgg gcaaaacaac
     1021 aaggcggaga actgattcga aaaatcgaaa atgaactaaa acgaaataaa aataaattga
     1081 aaaaacgtga acaaacactc aaagaatccg aaaacgctga gaattaccgt agaaatggcg
     1141 aactacttac tacttttctg acacaagtac cacgcggcgc aaaagaagtt gttttaccta
     1201 attattatga agaagatcgg ccaatcaaaa tagcactaga tccggcattg acaccaaatc
     1261 aaaatgcaca gaaatatttc catcgatacc aaaagctgaa gaacgctgtt aagctgattg
     1321 gcgaacaaat ccaagaagcc aaagatgaaa tccaatatct tgaatctgtc ttatctcaac
     1381 tggaaattgc cggcccaatg gatatcgaag caattaaaga agaattgact tcagaaggat
     1441 acctgaaaaa gaagaactta aaaaaacaaa aacgtaagaa accatctcag cctgatcaat
     1501 acttttcttc tgacggaacg ctcattcttg ttggtaaaaa caatcttcaa aatgaccagc
     1561 tgacaatgaa gacagctaaa aaaaccgact attggcttca tgcaaaaaat atacccggtt
     1621 cgcacgtcat cattaaaagt gacaagccct cagatgaaac aattacagaa gctgccgagt
     1681 tagctgccta tttttcaaaa taccgatact ctgcccaagt gccggttgat cttgtacagg
     1741 taaagcatat ccgaaaacca aatggcgcca agcctggtta tgttatttat gaaaaccaaa
     1801 agacgattat cgtgacacca gaagaagaaa aaattacagc tatgaaacaa aacaggtaat
     1861 caatgagtgt ttctagaatc atatttgcgt actttcccac aacagcaatt agttataaat
     1921 tgtttgctac aaaaagagga gttgtgacaa aattctgtca caacccctct tttttgatca
     1981 acggtgttca ataaacagcg ttctcatctg cttttcttag ctctgctggt ccagtaaccg
     2041 agtaagctct ttgataaatc tttgagcgat ttcactgagc ggacgtttcg ccgaataaat
     2101 gatataaaat tcatttagtt cgccttctgc caaagggatt aaccgcaagt gctcgcgaat
     2161 ccctccaatc acaataccag atcctgaagc gtaagcatca gtttcaagaa cgatattcat
     2221 caaagtacct cgatcactcg tataaatcac tgtttcttga tcagggatct cgataaggtc
     2281 ttcataaaaa tacgggaaat tatttccatc ctgtgtaaaa cgaacttgag gataagataa
     2341 caattcttct ttggtgatct ctgcgtgttc agctaatgga tgtccttgcc ttaaaaaaat
     2401 atgcgttttg aattttccaa tgctgcggcc acttagctct ccttgttgta aatatcttct
     2461 gataccagct tgattatgct cattcatata aataatgccg atctcactgt gctgctcttt
     2521 gacttcttcg atcactctcg ctgttgtcgt ctccatgatc cgaaattgtt tgatctcgtc
     2581 tttgaattcc tcgagtagct gtgcggtcaa aggacctaag aaatcataat gctgggcagc
     2641 gatcgaaaaa ttgtctctct tttcactcgt tagatagtga gtttctaatg tatcaagctg
     2701 gaccaatatt ttctcggcac gatttaagaa gtcttgccct tcttctgtta aatatgcccc
     2761 tcgattcgtt cgagtaaaca gcgtgatccc taactcggtt tctaattctt tgataccagc
     2821 tgaaagactt ggctgggaga caaacatttt ctttgccgct tctctgaaac taccattgtt
     2881 tgctacagcg accacgtatt tcatctgctg aatattcatt gctcaaactc ctcgaaataa
     2941 taaatttctc ttccattcaa gggctggatt ggtcggttgt tcggtcgagc atactgacca
     3001 tcgaagggga gaagaaactc ttggttcagt tttcctacat gatccattac aaaccattgg
     3061 attggcccgc tggtaggcgg agttgtcaat gagccaatat aatggaaatg gcttttttgc
     3121 tccggaaaga acacatccgg atttacccaa tgctcatgtt tttctaagtt ccaactttcc
     3181 ccattttctt gattatacag ccaaccttcc tgtaccatta gctggtacat gacacccaca
     3241 actagattct cttttttgct gttcatatga acaaaatgaa attcaatatc ttcctgtttt
     3301 ccgttgatga tatgttcaga aggcaagtgg aaatgaatat ctgtcaaata atagcgttct
     3361 ttcttgaact ctacataact gagttggtca aaaggtacta aatgaatcgt gtttttgaat
     3421 tccttgtcag taaaccgttg cttttgataa tgaaaggaaa gcgtgtctcc ttctattttg
     3481 gtttcttccc ccttggtcaa atgaataggc gactgcaatg gaaattccgc acctctcgca
     3541 aaccaatcac ataaactcgc ccaattttcc ggacctttgt ctccagaata gccccattcg
     3601 acatccatat tctttttcaa ctatacttcc ccctgcatct catatcattt ttatttttct
     3661 tctttcttta caatcgttga gacggtttct aaaaacgcct cttttccata aaattcctgg
     3721 ttcaatagtt ccggaaatat ctctttaaag aaatactgaa cagaaggccg atgttcaaag
     3781 cttttaatcg tagtcactgc ctcaatgaac atctccttat aaactttttc cgggttattt
     3841 ttgatgattt catcaaaaga ttcataaaga aggtcaacct tatctgaaat ggctaatagt
     3901 tctccttcta cggtttcatc ttttccttcg aacaaccgac ggcgataaac ttcttggaac
     3961 tcagctggaa tctcttggtt gatgaattcg tccgtcatct tttcttcaac tgtttgcagc
     4021 atccctctta actctttgct tgcgtatttc accggcgttt tgatatcacc aataaaacgc
     4081 tctgtgtagt catgattcaa tgatttttca tagagcagtt tccaatcgat cggttgtcca
     4141 ttcgcttctt caatgtctcc tagaacttgc gcaatagatg ctacacgata cgaatgtgct
     4201 gcaactgtat gctctgtata tttaaaaaat ccaggtgccc ttgtgatcgt ctctaaattg
     4261 ttcaatccta aaataaattc attcaacccc ataaaagtta cctccctttg agatttcttt
     4321 ttccgaatat catatcatta aataactgat attcgcaaca tttccgctct ttaccatttt
     4381 tgaaacagct cccttaccaa aaaatagaga aaatctatat ataatagaaa aaagagaaga
     4441 gattattctc ttctttctgc taaccagtct tagaaaaatg aattttaaaa aaatcaaaaa
     4501 ctgatcacga tagaagtttc ttttcatacc tgatgaaaag atgcttctcg caatcagttt
     4561 ttggattttt gctattccaa ttgatgattc tggagacaca aatcggattt ctctatcttc
     4621 atcaagcgcg cttaaatctg ccaattttca ggatcttttt tccattcttt tagcagagtt
     4681 aattcttgct catcgatata acgatcttct aaagctgctt caatcaatac agaataattg
     4741 gtcaacgtca taagagggat ttcggctttt tcgaaatttg cttttccttt tggcaactca
     4801 tacgtaaaga tagccgcaac accaaggata tccgcacctt ctctttttgc tgcttcagct
     4861 gcttccaaga cacttccgcc tgtagaaatc aaatcctcga tcacaaccat cttctgacct
     4921 tcaaatatac gtccttcgat ttggttacct ttcccatgat ctttcgcctt acttcgaata
     4981 tagaccattg gcaaatccag tatttccgct acccatgcgg catgagggat tcctgctgtt
     5041 gcagttccag caatcacttg tacttcaggg taagcttctt tgattttgct tgctaatcct
     5101 tttgcgatct cttttcggac ttttggatag ctcatcgtga ttcggttatc acagtaaatt
     5161 gggcttttga ttccacttgc ccatgtaaaa ggattacttg gatttaaaaa tactgcttca
     5221 atttctaata agtcttttgc gatactttgt tcaacattca ttttaggtta gctccattct
     5281 gtttgaatcg tttgatatgc tttataagga ttagttgact gagtgattgg tcgacctata
     5341 acgataaaag ttgagccgat cttttttgcc tgagaaggtg tgactactct tgtctgatct
     5401 cctgcgactg ttccttctgg tcttataccc ggagttaagc aaacaaattc gtctcctgcc
     5461 atttctttga tcgcttctac ttcccaagca gaagaaacca caccatctaa tcctgctttt
     5521 ttggcacagc tagcataatg gatcacactt ttttctaaag aaacttcgat tagttgatcg
     5581 gcatgcattt cttcttcact tgtagaagta agctgagtga tagctagaag tagcggacgt
     5641 tttccacctt ctggagtccc ctcttctaat cctcgcattg ccgcctccat catacgaata
     5701 ccgccagcgg catgaacaca ggtgagatca acaccaagct ttgccaatcc ccgcattgct
     5761 ttttcgaccg tattcggaat atcgtgaagt ttcagatcta aaaatacatt atgaccagcc
     5821 tcttttagat aacgtacgat ttcaggtcct tcttggtaga acaattccat tccgattttc
     5881 acaaacaatt tttcttcttt cgggaaaaat cgaagaaagt cttctacctc ttgcctagtt
     5941 gaaaaatcta atgcaataat tggtcgttgg tctagttgat tcatcgttca cgttcctctc
     6001 tcacttcttt gattaatctt tccagcgatt cgatgcctag ttcgctcatt cgcacaggga
     6061 gctctttaat tagtttagga cagatatacg gatcggtgaa attcgcggtc cctatagcta
     6121 cagcacttgc tccagccata aacatctcaa gaacatcatc cactgtctgc acgccgccca
     6181 tcccaatgat tggaaggctg gatatacttg ctacttggtg aatcaaacga atagcgacag
     6241 gcttgattgc tggtccggag agtccacctg tttgattggc taaaatcggt tttcttgtct
     6301 taaggtcaat ccgcatacca agaagagtat tgatcatcgt aaacccatct gcccctccag
     6361 cttcgattgc ctgagcgatt ggaacgatgt ctgttacatt tggggacaat ttgacataga
     6421 caggaacaga agaaactttc tttactgctt gtgttaattg aaaagcaaca tcagaatctg
     6481 ttccaaaagc aattccgcca tgtttgacat ttgggcaaga aatgttcagc tcgattgctt
     6541 tcacatttgg tgcttcgcca attttgctac aaacttctac atagtcttct tcacaagcac
     6601 cagcaacatt tgcgatgatg ggtaactcat tgtactcctc taattctgga agaagtttat
     6661 gcataacaac gtctagacca ggattttgca aaccaattgc attcaacatg ccagatggtg
     6721 tttccgccac tcttggcgtg ggattgccaa agcgggcatt tggagtcgtt gccttgacca
     6781 tgatcgatcc aagctgattc aaatcatagt attttccgta ttctttgcca aaaccgaaac
     6841 agccgcttgc cggcatgata ggatttttta attctaggcc aggaagtttg accgctaaag
     6901 gattctcatt catataatta cctctcctat tttaaaaatc ggtccttcgt cacaaacttt
     6961 cacacttttt gttcctgttt catcttccgg cgtatgacaa acgcaagcat agcaagcccc
     7021 tatcccacat gccatgcggg attctaatga taactggaca ttttgatgcg aagaaaacag
     7081 ctgttccact gtcttcaata acccgtttga accacatgca aaaacagctg taggttccgt
     7141 cttcgttcct aacaataaat taccgacatt tccgtgcata ccgtatgttc catcgtcagt
     7201 agcgattctt gtttctccta gacgattaaa ttctttttca taataaacga cttcatgtgt
     7261 ggcaaaacct aaaaaatgcg ttggcttaat tcctttttcg actaactgtt ttgataactc
     7321 atatagagga gggacaccga ttcctccacc gattataaat acttcgtctc ctgtatgaag
     7381 attggacaaa tcaaaaccat tgcctaaagg acccatgaca tctaaatggt ctccggcaga
     7441 aagagtagag aagactctag tcccctcacc ttccacccga taaatgatac ggcaagtatt
     7501 tgtttcgtgg tcgatatgat tcaagctaat cggtcggcgt aatagcaagt ctgcacgcgg
     7561 cacgagaata tgcaagaact gtcctggttg ctgcatttct tttactagat cacctttcaa
     7621 tgtcatttca aaaatctttg gtgccagctg tttttgctga acgatttcca tcatttcttg
     7681 tttcatctgt cccctcctgc aacggttttt cgtctttttg tcttaacatg tgctcgccgt
     7741 taaatagggt tagttgaaaa agcacgggat tccatcactc gaacgatcgc atctgctgtg
     7801 tctaaagaag taaacaatgg aataccatgt tcaactgctt ctcttcggat gatgaaaccg
     7861 tctttagaag catttccccg atctttatca atcgtattga caaccacttg tgttttacca
     7921 tctcttatta agtcgactac atttttttct gctgcttcct cagaaatttt cgctactgtt
     7981 tcaacgacca gaccattttt ttcgaaataa gcagctgtat gtttcgttcc tatcagacta
     8041 tagccaattt ctgtaaaacg tttagctaat tctagtgctt cttcttttgt ttcgtccgca
     8101 atcgtaaata atacggctcc atattcaggc aagtgtaatc cagaagcttc gaatgccttg
     8161 tacaaagctt tgtctaattg tctgtcagat cccattactt ctccagtcga tttcatttca
     8221 ggccctaagt aagtatcaac tttctgcaat ttcgtaaaag aaaagacagg tgctttaaca
     8281 tgaacttctt ttgtttctgg ataaagtccg ttttcataac caagtgtctt tagtttctct
     8341 cccaaaatag ctttcgtcgc aatttgtgcc attggaattc ctgtcacctt acttaagaat
     8401 ggaacagtcc gacttgctcg aggattgact tcgatcacat aaacctcatt ttcatggatg
     8461 acaaactgga tattcatcat cccgacacaa ttcaaaccta atgctaagcg gatcgtgtaa
     8521 tcttcgatcg ttttcttgat cgattcagat aatgtttgag gtggatacac agccattgaa
     8581 tcccctgaat ggacaccagc acgctcaata tgctccatga tccctgggat gagtactgtt
     8641 tcaccgtcac aaatcgcatc cacttcgcat tcaccaccaa gcaagtaacg gtccactagt
     8701 actggatgtt ctggagatgc tttcacagca tgttccatgt aatcttctaa atctcgctgg
     8761 ttttcaacga tttccatcgc acgtccgcct aatacatagc ttggacgaac aagaactgga
     8821 taaccaattc tcgaagcgat aaccacggct tcttctttac ttgtagcagt atcccctaaa
     8881 ggttgaggaa tatccaactc tttcagcgct tgctcgaata aatcacgatt ttccgcacga
     8941 tccaagtctt cgatcgttgt ccctaagatt tttacaccag cagcagtcaa aggttcggct
     9001 aagttgattg ctgtctgacc accaaattga acgattactc ctatcggctg ttctagctcg
     9061 atcacattca tcacatcttc gaaggtcaat ggctcaaaat ataatttatc cgaaatagaa
     9121 aagtcagtcg aaactgtttc aggattgctg ttcatgatga ttgcttcgta tcctgcctgc
     9181 tggattgctt tgacagaatg cacagtcgca tagtcaaatt caaccccttg tccgatacgg
     9241 ataggaccag atcccagtac tagaattgag ggcttttctg atcgtgaact ttcattttca
     9301 tattcatagg aactataaaa ataaggtgtt tttgattcga attccgctgc acaagtgtca
     9361 accatcttat atacaggaat caaatcattt tcttttctca tctggcgaac ggcttcttcg
     9421 tttatttccc acagctccgc cacttttttg tctgtaaaac cgttttgttt cgctgttttc
     9481 aatatcttgt catccaacgg ctgctgtttc aactgctgtt cgatttctac gatatgcaac
     9541 agtttatcta aaaagaataa atcgattttt gtcaaatccg ctaattcttc tatggtgtac
     9601 cctcttctaa tagcttcagc tacataaaac aggcgatcat cttgtgctcg aaccactttt
     9661 tggattaggt catcgtccgt gatgtctgcc aactcaggaa gttcgatatg gtgtgtaccg
     9721 atttctaaag aacgcacagc tttcaataaa gactcttcga tattccgacc gatagccatc
     9781 acttcaccag ttgctttcat ctgcgtgcct aaaagccgtt ctccgttttc aaacttatca
     9841 aacggccagc gaggaatttt agctacaaca taatctaatg caggttcaaa ttctgcaaaa
     9901 gtcgttcctg taacaggatt tttcatttcg tctaatgtta atccaagcgc tattttagca
     9961 gctagcttag cgatcggata gccagtcgcc ttacttgcta acgcagatga acgagaaaca
    10021 cgcggattga cttcaatcac ataataatta aaactatgtg gatcaagagc gagttggaca
    10081 ttacagcctc cctcgatctt aagtgctcga ataatcgaaa gagaagcatc acgaagcatt
    10141 tgatattcat ggtcagacaa tgtttgacta ggggcaaata cgatagaatc acctgtatgg
    10201 atacccaccg ggtcaaaatt ttccatattg cagacaacga ttgcattatc agccgaatct
    10261 ctcatcactt catattcaat ttctttaaaa cctgcaatac ttcgttcaat caaacattgg
    10321 gtagcggggg aaagacttaa tccattttct gatacgatac gtaattcttc ttcattttca
    10381 cacatacctc caccagttcc gcctaaagta aaggctggac gaacgatgac aggatagccg
    10441 attttatttg caaaatctac agcttcttct actgtattga cgatttcaga ctcggggatt
    10501 ggctgattca attctttcat caactgtttg aatagatcac gatcttctgc ttgatcgatc
    10561 gcagaaagct ttgttcctag aagttctatg ttcaattctt ctaaaatacc tgatgctgcc
    10621 agctccattg ccatattgag gcctgtctgt ccgcctaaag ttggaaggag agcatctgga
    10681 cgctcttttc gaagaatcct tgatacaaat tcaagtgtga tcggttcgat atatacttga
    10741 tctgccactt ctttatccgt catgatcgtc gcaggattag aatttactaa aaccacttca
    10801 tatccttctt cttttaaggc aaggcaggct tgtgttcctg cgtagtcaaa ttctgcggct
    10861 tgaccaatga tgatcggacc agatccaatc accataattt tcttgatatc tgtacgtttc
    10921 ggcatctgtt tactgctcct ttcctgcgtc catcatttcc ataaattcat caaataaatg
    10981 tagtgcatca tgcggaccag gggcagcatc tggatgaaac tgcacactga aagcgggata
    11041 atctctgtgt ttgactcctt ctactgttcc gtcatttact tctacatgag ttacgatgag
    11101 tttttctgga tcaatcgtgg cttcatcgac tgcatagcca tgattttgag aagtaaaatc
    11161 aattcttccg gtcgctactt ctcgtaccgg gtgatttaat cctcgatgtc cgaatttcat
    11221 tttataggta tctgcaccgt tggctaaagc aaataactgg tgaccgagac aaataccaaa
    11281 aatcggtact tttccttgga tttttttgat catttcgatt gcttctggca catcttttgg
    11341 atcaccaggt ccattcgtca gcatgactcc gtctggtgat agcgctaaaa tcgtctcggc
    11401 atctgtgtta tagggaagta ctgtgaggtt acagtttctt ttactcaatt ctcgtaaaat
    11461 actatgtttc aagccgaagt caatgacaat gactttccga cctgttcctg gacttggata
    11521 aggtttgtta gttgagactt gctgcacttg attggttgct agaacggttg ctttcagctg
    11581 atcaaatgca tgttcgaagg aatctccagc atcaactata ctagctttca tcgttccgac
    11641 ttcacggatt ttgcgagtaa gggcgcgcgt atcgatacct gatattccag gaattccttt
    11701 ttgtttcaaa aatgaatcta gcgtcatttg gtttcgccag tttgaagcta cacgtgcatg
    11761 ttcttttact actactcctt tgcatgttgg agaaatcgat tcataatcat ctctatttac
    11821 cccataatta cccaccatag gataggtaaa tgtaatgatt tgaccgttaa aactttgatc
    11881 agtgatagtt tcttgataac cagtcatact tgttgtaaaa acgatttctc cgaagacact
    11941 agcatctgca ccaaaagcct ttccttcgaa aactgttcca tcttctaata taagcaaacg
    12001 ttccatactt ttattctccc ttctgccaca caagttttcc gttgacaaaa gtcatttgtg
    12061 tttcgccttt tactttccag ccgataaaag gtgtattctc cccttttgat aaaaagtctt
    12121 ctttatcgat cgtgcaagtt tgtgtaatat caaaaactgc tacatcagcg ggggcaccaa
    12181 ccgttagcgt tcctgcattc aaaccaaaaa tctcagccgg tttaactgcc aaccaattga
    12241 tcacttgttc caacgtgaat cgaccagttt ctacaaaatg agtatagatc agttgaaacg
    12301 ctgtttcgct tcctacgata ccaaatggtg atttcatgaa actttggctt ttttcttcta
    12361 atccatgggg ggcatggtct gtagcgatgc aatcgatcgt accgtcaagc aagccttcga
    12421 tcaaagcttc tctgtcctcc cgtcctctta gcggcgggtt cattttccaa aagccgaaat
    12481 cttcgggaat atcttcgtcg atcaaaataa gatgatgggg tgaaacttct gctgttacgt
    12541 ggattcctgc tttttttgcg tctctgatta cacggacact ttctttagta gaaacatggc
    12601 aaacatggta atgaacacca gttgcttcag ctagcaacag atctctagca atttgagaag
    12661 actcggttac gcttaagatc ccgggaagac caagttcttc tgccttcttg ccagcgtgca
    12721 taacgccgcc aaataataaa gattcatctt ctgtatgggc tactagggct ttattgtttt
    12781 ttgcagcttc tttcatagcc agatacatcg tcccagctgt ttggacacct acaccgtcat
    12841 ttgtgaaagc aaaagcacct tcttcaatca gtgcttcttg atcaactaat ttctcacttc
    12901 gcagattttc tgtgattggc gcatactgca ataccttcac aactgcatct tttctaatga
    12961 tgtcatatac ttgtctcagc ttttctgccg tatcaggtac aggatttaaa ttcggcatcg
    13021 cgcatacagt agtgaaaccg ccacgagcag ctgagcgagt cccagcttcg atcgtctcct
    13081 tgtatgtaaa tcctggctct ctcaaatgaa catggacgtc cactaaacca ggtgtgatca
    13141 attgtccctt tgcatcaaac acttcatcaa attcggcttc tgagaatcct gtaccgattg
    13201 ctttgattcg cccatcttct atccaaattt ctgctggtgt cgtcatattt tttcgtgtat
    13261 tgatctgacc gtttttaatg agtgttttca ttttttcatc ctcctataat tacgcttttc
    13321 catttagcac tgcttctaag attgccattc tgacgaatac tccattgctc atttgctgaa
    13381 cgatccgcga ctgtaatcct tccactaatg aatcagctaa ttctacatca cggttaactg
    13441 gtgctgggtg cataatgatt gcatgttttt gcaacttctt cgcacgctca actgttaacc
    13501 catgttcctg atggtagcct tcttttgaga aactttcttt tccatcatgg cgttcgtgtt
    13561 gcacccgaag catcatcatg acatcaactt gatctagtag ttcatccaat ggcatataat
    13621 gtccgtacac ttcgaattca tcgtcgtacc attctctagg acctgagaag aatacttgtg
    13681 cgcctagacg ttttaacatc tgcatattgg atttagctac gcgagagtga gtgatatcac
    13741 ctacgatggc aactttcaga ccttcaaaat gaccaaattc ttcataaatg gtcatcaaat
    13801 caagcaagca ttgggttgga tgctgtccgc ttccgtctcc tccattgata atcgaacact
    13861 ggattgtttt actttgtatc agttcgtcat aatagttctc atcaccatga cggataaccg
    13921 ctacgtctac gcctaatgct gacatcgtca gtactgtatc gtatagtgtt tccccttttt
    13981 ggacagagct tgtgcttgct tcaaattcaa tcacttctag ccctagtttt ttctccgcta
    14041 catcgaagct tttatgtgtg cgagtactat tttcgaaaaa gaggtttgta gcaaagtact
    14101 ggcgcttttg cggagtccaa ttagcacctt gcttgaattc ctgccctctg cggatcaagc
    14161 ccataacttc ttgatcagta agtgcctcaa cggtcaataa atgctttaag ctgatacgtt
    14221 ctgatttgat gatcataatg tttcctcctt agttttcttc gcttcgtgaa gtttctggta
    14281 agataagatt taaaatgatg ccaagtacag ttgctaatgc cattgccgac aatgtaaatg
    14341 tgcctacttc aaagacgagt ccgccaattc ctactactaa gataactgaa gcaatcaaca
    14401 gatttttctt tttatcaaaa tcgattttgt tctcgattaa aatcttcatt ccacttgcgg
    14461 caattactcc aaataagata aaactgattc ctgagatgac tggtccaggg atacttaaaa
    14521 tcaaagcgct taattttcct acgaatccta aaatgactgc aaatactgca gctccaccga
    14581 tcacaaacac gctatggaca cgtgtgatag ccagaacacc aatgttttca ccgtaacttg
    14641 ttacaggagg acctccgaca aaggctgcca cgatttgtgc taatccatca cctgtcaacg
    14701 tcttatgaag acccggttct tcaaagaaat tacgtttagt tagtttattc aataccataa
    14761 gatggccgat atgctctgtc atcgtcacaa aagcgatggg tgccattgtt gtgattgctc
    14821 caagatatag ctttggttca tattgtacaa agggaatctc aaagttagga acagcaaacc
    14881 aggcagcgtc tacgattggc ttagtatcca cgattccaaa cagcaaggct acgatgtatc
    14941 cgctaataat acctagtaaa atcggaatca atccgagaaa acctcttagc cacatgttga
    15001 agaaaattgt taatcctaat gtgatcagcg ctacagcaat atacttgaaa tcatacgtgc
    15061 cctgattata catagcattt gttgctgcat ttgccgcaag tcccaacccg ataaccatga
    15121 tgactggacc gacgacgatt ggaggcaaga ttttatccag ccatgctgaa ccgatctttt
    15181 tgatgatcag cgcaacaatc aaatagacta atccagttgt gactgctcct tgtgcaatcg
    15241 ctggataacc atctgttttc atcaaaagct gcatcgctgc aatgaaagcg aagctgcttc
    15301 ctaagtacgc aggtatcttt cctttagtag taaataagta caccaatgta cctagtcctg
    15361 aactgactag cgcaatacct ggatcgatac ctactaagat ggggactaac acggttgctc
    15421 cgaacatcgt gaacaagtgt tgtaaactta atcctaccca atgtcctggg gttgggcgat
    15481 cgtggatatc taacaccacg tcatcattat gaaatccttt tttctccgac atgtttctct
    15541 ctcctctaat cttcctcttt caaaatcatg atgcgatctt gtccatcaag ctcttgcatc
    15601 tctactaaaa tctcttccgc ccgagatgtt ggaatgttct tcccaacata atctgcacgg
    15661 attggcaatt ctcgatgccc acgatcgacc aggactgcta atgaaatttt tcttggtctg
    15721 ccaaaatcca ttactgcgtc cattgctgct cgaatcgttc gtcctgtata caaaacatca
    15781 tcaatcaaaa taacttcttt tccttctaag gaaactggga tgtcagaaga atgaagttcc
    15841 ggttcttctg gattttcttt tttgtcatct cggtataaag tgatatccag ttctcctaca
    15901 ggaatatcga tatcttctag ctgcttcagg cgttcggcaa ttcttgatgc aatgtagatt
    15961 cctcgtgttt tgatacctac caatacgata tcttggatac tatggtttct ctcgataatc
    16021 tcataagtaa ttcgagtcaa tgctcgcttc atggttacct gatctactac ttcttttgct
    16081 tgcattttca ttccccctac ttttttgcat aaaaaaactc ctgccctaag aggacaggag
    16141 gaatgtttag actgagcgta tactgataca ctgatagcta tactatgtca atgagcaatc
    16201 tacttatggc gcaccatacg tatccttgct cccacccttc ttagcctcac gggactaaac
    16261 ttaaaggaat tctattaagt tttatgcttt cgttgttaat tactataccg ttatctaaat
    16321 taaaatgcaa tgttttttct taatttttct aaagtttttt caaaaacttc tggaagaggt
    16381 gcttcaaaga tcatcttttc gtttgtagtc gggtgcgtaa aacctaacat ttgcgcatgg
    16441 agaaactgac cattgccttt caaagttttc ttcggtccgt atactggatc tccagctaac
    16501 ggataaccga tatacttcat atgcacacgg atctggtgtg ttcttccggt ttctagtttt
    16561 aattcaacca gagtaaatcc ttcgaaacgt tccaatacag taaaatgtgt gacagccgac
    16621 ttcccatctt ctcttatcgc ctgcattttc cggttttcct tcgaacggcc gattggcgca
    16681 ttgatggttc ctttttcgtg aggaatctct ccatggacta aagcaacata tttgcgcaaa
    16741 gaggtcttat cttttaattg ctgagctaat gattcgtgtg cctggtcatt tttggcaacc
    16801 attagcagtc cggaagtatc tttgtcgatt cggtgaacaa tacctggacg aataacgccg
    16861 ttgatcgtag aaagatcctt gatatggtac atcagcgcat tgaccattgt gccatgcgta
    16921 tgacctgctg aaggatgaac gaccattcct tgaggtttgt ttatcaccgc tacatcttca
    16981 tcttcgtaaa cgatcgtcaa cgggatattt tctgggacca catctaatgt ttctggttcg
    17041 gggatcaaga cagtaatctc gtctccttgt tttactttgt aatttgcttt gactggctgt
    17101 ccattgacag aaaccgctcc atttttcagc cacagctgaa tttgcgaacg actgtaatcg
    17161 gtgaataaat tagtcaatac tttatcgatc ctaccagatt catggtgaat cgtcgtttta
    17221 agttctgtca tttttttctt tgatcctttt catctaaaat aatataaatt aacatacaaa
    17281 tcactccaca tacaagtgtc acatctgcta cgttaaaaat cggaaagctc ataaaatctg
    17341 tctggaacat atctactaca tagccaagac gaatacggtc gataaaattg ccaatcgcac
    17401 cagctagaat caaactcaat ccaatcatga accagcgatt acctttttca cgatttttaa
    17461 tcatcaatgt gagaatcaca acgactgcaa tgagtgtcac tatagtaaag aaccacatct
    17521 tcccttctag catactccat gcggctccag tgtttcgaat atgattcagt gacaaaatac
    17581 ctggtataag atttttcgtc tctccaagcg ctagattatc agtgatccac catttgacca
    17641 actgatccac tcctactagc aaagcgctga atatccaata aataattaac aaaaaatcaa
    17701 tccttttcta tttgtttata aatatcacga ggtcgtacga tcccgagcag attaccatcg
    17761 ggattcccat ctttagtgat aagtacggct tccaaagtcc cttgctcacg aaacatcgct
    17821 tcaacctcgt aaatatgtgt tttagctgga acaaaatgat aatttgtgta ctttccgttt
    17881 tggatgatca aatccaacgc tcgtttattt gttaaatcaa ccggaccttt ttggctttct
    17941 tttgctagcc aaaacccgat attacgtaaa gtaatcaatg ctacaaattt tcctttattg
    18001 tataacggaa attgtgaata ccttttttct gctactatct ttaacagttc aagtaacgga
    18061 agatcttgtt caaaacctgt tacacgtttt gcaaatcgag gcagcacggt ttcaggctga
    18121 atcagttctt gttcgatcag ctggattcgc ttgactgtcc aatcatttgg ctctgcaatg
    18181 acaaaatcga ctgcgatccg atcatggacg atcgcattcc ttaactgagc aagctgtaaa
    18241 agatcatctt cgtattttct gatcattaat tgttttcttt gagctaggcg tctgacaagt
    18301 tcggtaaatc ccatatttcg tgcatttccc atctcgtctc tcatccattt ttcgattcga
    18361 ttgaaactga ttaaaaaaag atcagagttt gccataattt tcctccttga tcaaatatgc
    18421 ccggtttttt atatagaaaa agaacgcaat caacacaata actgcaaaaa aactgaaaaa
    18481 taaattttgt aggaaaacgg cccccaaccc agttgctaga aataaaaacg gatagatttg
    18541 aagaatcaag taattcattg tgatcacata agcaggcttt gcttctttca ctgtataaac
    18601 ggggcttttt agccaataaa aggtgacctg catcgtaaag ggcacttcca gatcaagcgt
    18661 tatcttttgt tcatgactga gaacactgcg tttttcattg ttgattctta gctgaatcga
    18721 gctacccata ccgtaaaaac cggtttttct tataaatgtc gttttcatca taccctccta
    18781 ttatgcttta gtatagctca aaatacaata aagaaaaaga atgatcctag tgtaaacaaa
    18841 attaattccc tatcgtctaa aaaaagaagc tgtagtcaaa atacagctcc ttcgatggta
    18901 gattataatc aagcttaaaa caatcccaac acttctccgt catttgtcac gtccatattc
    18961 aatgcagcag gttttttagg caaaccaggc atcgtcataa catctccagt caaggctact
    19021 aaaaatcctg cccctaattt tgggatgatt tcgcggatcg tgatggtgaa atcttccggt
    19081 gcacctagca gtgaaggatc atcagaaaat gaatactgtg tttttgccat acaaatcggc
    19141 agtttgtccc atccattctt cgtaaactct tctatttgtt tttgcgcttt tttgctgaaa
    19201 acaacaccat ttcctccgta aattttcttg acgattgttt ctgtcttttc ttgtatcgtt
    19261 tcttctaggc ggtataatgg cttgtaatta gcttcttttc tatcaatcaa tcgtactaca
    19321 gcttttgcta aatcaacgcc gccttctgct ccatctgccc atacactcgc tctcttggca
    19381 agaatcccct gattttcaca caagtgctcc aacagtgtga gttcattgtc tgtatctgtt
    19441 acgaattcat tgattgcgac gatcacaggt agctgatatt gttccatatt acgtatatgg
    19501 cgctttaaat ttgcaaaacc tactttcaaa gcatcaagat tctcattttt aagttcatct
    19561 tttttcattc cgccatgcat tttcaatgca cggacagtgg cgacaatcac cacagcatcc
    19621 ggcgcttttt tcaagttagg tactttgata tctaaaaatt tctctccgcc aagatctgca
    19681 ccaaatcctg cctctgtgac aacgtaatct gctaatctca atgctgtctt ggtagctaaa
    19741 atactattgc agccatgagc gatattggca aacggtcccc cgtggacaaa agctggtgtt
    19801 ccgtaaattg tttgtacaag attcggcttg attgcatctt tcagcagtaa tgccagagcg
    19861 ccttctgctt tcaagtctcc agcagtcaca ggctgacggt caaatgtata gccgacaacg
    19921 atccttgcaa gacgagcctt taaatcttca aggtctgccg caagacacaa gatagccatg
    19981 atttcgctgg caactgtgat atcaaaccca tcttctcttg gaacaccttg gattggccca
    20041 cccaagccaa caattacttt acgcagttca cgatcattga gatcgactac ccgtttccaa
    20101 atcactcggc gggcatcgat gttcaattca tttccttgat gaatatgatt atctaaaaga
    20161 gcagataatg cattattagc tgttgtgata gcatgcatgt caccagtaaa atgcaagttg
    20221 atatcttcca taggcaatac ctgtgcatat cctccgcctg cagcaccacc tttgattccc
    20281 atcacaggac caagtgaggg ttctcgcaat gcaataacag attttttatt gattttattg
    20341 agcgcgtctc ctaaccctac agtgatcgtt gactttcctt cgccagctgg tgttggattg
    20401 atggaagtaa ctaagatcaa atgcccctca gcgttatcct ttgatcgatt gattccagaa
    20461 aaattgattt tcgccttata tttcccatac agttccaaat cgtcttcctt aagatctaat
    20521 ttactagcca cttcaacaat cggttctagc tctgtttctt gtgaaatttg cagatcattc
    20581 ttcatttgat tacccctttt cacgaacttt attcaacaaa atatcttctt tgttaagata
    20641 tgccaattat accttttttt attcagaaaa aaaacataaa attacaggtt tttaatttat
    20701 ttgacgtact tataaatact ctttataatg aaggctatga tcagacttta gaaaggtcgt
    20761 gagtgctttg accacaggaa ttgtaaaatg gtttgacaac aaaaaaggat acggctttat
    20821 cagttatgat gacacagaag aaatttttgt acattttact gcgattgaag aagagggatt
    20881 caaaactttg gaagaaaatc aagtggtcga atttgagatc atcgaaggga atcgaggaac
    20941 acaagctgcc catgtcaaga aaggcaacgt tcagactacc gatactagta tgcatgaata
    21001 gataaacaaa tagagtgaga cgctaaacgg gatgaaccgt acgtctcact cttttctttt
    21061 cctgtaaaaa ttcgtttttt taggttctga tttttaaaat tagctttttc tttttttcgt
    21121 ataaggataa ggaattgatt ctgatttggg tccgtttttt tcaaaatgat caaaaaggac
    21181 tacttgatct ttttttcgca tcatgaaaac agatggggct ttctcatctt gcaacatcat
    21241 ttgcacccca gtattagttg gcgtcaaaga tcgaatctgt ccgacagata tttctcggtc
    21301 tttccaaaaa gcagcataac gtataatcac agagtttgat tttaaaataa aagaacgccg
    21361 caaacctaga aatagaaaaa caagaaaaat acctaaaaca aaattgcttt tccaataagg
    21421 acctgtgttt tctaaagaaa gaatcagact aaaaaacaga atgatcaagg tcaatgacca
    21481 atagatgatc gtatgtgcca gttcaggctg ccatcgaata ttttttttca ttctctaacc
    21541 tctcaccgat tcagtaatga tataatctta ccataacatt agtttgaaca aaaaggagaa
    21601 tctcttttta tgttaaaagt atacattgat gcctctacaa aaggcaaccc tggtcctagt
    21661 ggaggtggaa tcttgattgt ctatcaaggt aaacaagaac agctatctat cccgctttct
    21721 atagggacaa accatcaagc agagttcgaa gtctttttaa aaactttaga atttctaaag
    21781 aaaaacgact tacagaatga aacgattttc tgtttttcag acagcaaaac actagtttct
    21841 acggtcgaca aaaacgcaac gaagaacgag ctcttccttc cctaccttca aagaatccaa
    21901 gaacttctat ccgaattttc tttattgatc ctgcaatggt tgcccgaaaa caaaaataaa
    21961 ggagcaga
//
LOCUS       DASHHB010000044        21319 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.045, whole genome shotgun sequence.
ACCESSION   DASHHB010000044 DASHHB010000000
VERSION     DASHHB010000044.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21319)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21319)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21319
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.045"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..138)
                     /locus_tag="U2K71_001931"
     CDS             complement(<1..138)
                     /locus_tag="U2K71_001931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM7315984.1"
                     /translation="MTYTHLTTDELVIIESYFKINQSVAKTAHCLNRSRQTIHKVYLF
                     FK"
     gene            678..1601
                     /locus_tag="U2K71_001932"
     CDS             678..1601
                     /locus_tag="U2K71_001932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315985.1"
                     /translation="MATNYAKKINFDVDKLYKSLIVVPKNKSEDGNYELEVLQFKIEQ
                     KSSERFMSNQLVKIKKWLPMIDQLVSNNKEAKNLCIFVLLLHGRVINLVNRKSTTKFA
                     NSFWFLENINETVEKNGVYKGDFHFQNAETIEREHHYFDLLVNTVVELAKWAKSNETY
                     SQVLKRIMSQETSFIYELSKSNLKYTYNEDELEILDNPENSQWIKELKREDFIETLRI
                     LKSLFDLKLAKATIMGIERDSYYWEEIGLYAPQIEGEDKEKRIDNIINYINSLSEPLK
                     CRGLDFIGTLGWTETYYEKIYKEIIEQYFPK"
     gene            1784..4459
                     /locus_tag="U2K71_001933"
     CDS             1784..4459
                     /locus_tag="U2K71_001933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF927 domain-containing protein"
                     /protein_id="HEM7315986.1"
                     /translation="MKMNHTSIQKHLQLLNTLGISYVNLRTFSTLNKKTVSHIFSTAD
                     SQAIINYLEKQKLSEGTVNITYNPIKQEVITQKKHSVRDNDIEKIRFVFIDIDPKRKE
                     GSATDSEKKKAENVMEQVERYLKEKRIESFVKVDSGNGYHIFLPINEQPNNHETILTI
                     QNFLQLLHRRFGVEDEVDIDTSVYNPSRLCKLVGTPAVKGKSTKERPHRLSSFLSIPD
                     NIDRSNCFKILEQIVEENSMMSTSRVKSHPSFREVLPFIQADYKQWLAHYPELTYTIK
                     EGDHKGVTLLIFDECPLRKHTNNSNGSCLSVTADNKIRFHCLHASDKNKDIQDFVKKY
                     PLPKPVNPSVESLESNQEYYFDQFKLNQEGVFLVKEGQEEKVACPMFIKQIRKNIEQK
                     VITLVLAYYVDHCWDEIEIEGGCLQTNNFKQLAKHGLIIKARREAEVVDFLQIQRENA
                     PIFFEHESLGWKKENEAMIFQLQDSFGREIPSVLTKNSCYQLTTNGTEAEFDALVHKY
                     VLGTNLELAWSIGIASIVLGYLNVTKANTFLSLLINLQGKSTTGKTTTLHFIASLFGN
                     PTTVLRNMNATNKAIIKLASNNQGGVPLILDELGAATQNNFSSLIYQLASGEERLRLD
                     SNCQLMPQETFSVLTLTSSEERLENYFNEKLTGLKVRYIEFSDIKFTKDANHAEKVKS
                     LSSNTFGWGIKLFLLKLFEQGIAKIEATFVEAQKKIWAVLPEHSLKQRIGNNLALIYS
                     GALLIQDILGYPLRLEHIQTVLINSYEQLIVDTSLGSEQTVFDRVKTLLVMNSHKFLT
                     NSDNKHTPSTIWGKVSIQKNGDIKVNIFPIPFEDLLRREFQVKDLTYLFRQLLDSGNM
                     CAEKGRRTKRIHMNRQVLPTYEILLPSSLKSYFRL"
     gene            4641..4880
                     /locus_tag="U2K71_001934"
     CDS             4641..4880
                     /locus_tag="U2K71_001934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315987.1"
                     /translation="MTKNKMTLKAEVLLYIQEHFSNQAFFTKPIYLAFEIRGVSAGSI
                     GGTLQALKNEGYLENHFVQRSFNGRVAKEWYLVHS"
     gene            4896..6269
                     /locus_tag="U2K71_001935"
     CDS             4896..6269
                     /locus_tag="U2K71_001935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7315988.1"
                     /translation="MRVACYDRTSVYEEAKISNDAQYDAVKEYVLEKYEQIPQRFSDK
                     GESGGKADRPAFRQLRAWVCASEEEGVIYVWRYDRLARQAKIALEFIELCQQHQVQVY
                     SISEPLPASFSHASTNKLFVQLLFTFAEQQRNVIIENVRAGLAYKKQQRQYLSSAVPY
                     GYRLVNGKIQQEAHEAKVVRHIFQLYLTKKYGYKKLCQRLTQQKFFFRERPFQPYHIY
                     SILKNPLYYGEIKGGSLGKYLGTFEPILSKTIFLQAQEIRQSRCTAKKDTYPYLLRQK
                     IRCPFCGRHLSSKYQWNTKKTKTLHYYHCTDRSCQGIFLRAERVESEVLEKIQAFFQY
                     EGVYQALIAQLTQQLAEKRKDEKHKVIQQKKQEAQWLKAFEEGNCSLEELKQQLQALQ
                     KPRSTSSFLSLQQCEAKVKEWLQLKDLSSRQLLCDHVCRIEVTKEKTVCGIYFRQMTQ
                     NILEGNE"
     gene            6273..7910
                     /locus_tag="U2K71_001936"
     CDS             6273..7910
                     /locus_tag="U2K71_001936"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7315989.1"
                     /translation="METTPTKRVALYIRVSTQEQALEGHSIQAQEQNGRQFAERMSYE
                     VVEVYADEGISGKSTKNRFAYQRMMNDARNHRFDLLVIWKLTRLGRNMLDVLQTVEEL
                     LRYDVGLHSISEQFDVTTSTGKLMLQLLGSFGEFERNQIAENVQMSMQSLVKDKKRYA
                     GGRRLGYVSGIDTNGQKQLVIEPEEAKIVRLIYAKYLGGAGYRAIANFLNRQGYQTVK
                     KNAFSTTAVKDILRNKIYAGYLEYARYINWETKRRKGKNPKPILVKGTHEPIISEEDY
                     QKVQERLVLEGKQPKWNHTGENVLTGLLRCPECGAPMAASNVTNTLKDGTKKRIRYYS
                     CSVFRNKGASVCHANSIRAEQAETFVADRLKEIVQLPEVLPRLVAALNEEIVRQSQPL
                     EQELVVLLERKEELKTKIEKWEAALEDSPELFPMLKDRLDELTEKRRQLHIRENEILG
                     IFQQQGEPIQVKDVQRILTSLDRFLAQSEKKQVKALYRTFIEKITFDPNYKEILEITM
                     KFTPAVVQQLNEQYQIAVSKTKDTAIFVLKTPFTLTI"
     gene            8073..9005
                     /locus_tag="U2K71_001937"
     CDS             8073..9005
                     /locus_tag="U2K71_001937"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7315990.1"
                     /translation="MKKMGKCILCEKYTELTKEHVPPKKSGNTGGKKTRTGNLDDFLK
                     SDFTKGDFPKGIKRKPQGNVYYTLCSKCNSFFGSEYVEEYIRFAEDNKNFLYNNTSLK
                     NGKSDLTHSIKKMNSLRVAKAIVAMFFSLNGDEDSMDKPFLDSVREYLSNPKSTLFPH
                     EDYKIIMNYYLPIGAPQNSKIKIPNDIVNDGDTHNVIKGFMPLDLSTGRKIRFSEIQY
                     EGIGFTLIDLKNSTFEIKEGLDLKNLLCADDKIKKIQLYKVPILTPTVLTMLEMENNI
                     NYIDSGSDLLIRWQVITNFLMELKKYSNTQTKFS"
     gene            9130..9387
                     /locus_tag="U2K71_001938"
     CDS             9130..9387
                     /locus_tag="U2K71_001938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP pyrophosphokinase"
                     /protein_id="HEM7315991.1"
                     /translation="MEDSPELFPMLKDRLNELTEKRRQLQIRENEILGIFQQQGEPIQ
                     VKDVQQILTSLDCFLIQSEKKQVKALYHTFIEKITFDPNQK"
     gene            9619..10023
                     /locus_tag="U2K71_001939"
     CDS             9619..10023
                     /locus_tag="U2K71_001939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit M
                     N-terminal domain-containing protein"
                     /protein_id="HEM7315992.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYAISPEYLFNVLA
                     DQAKQATFQLNDLNKAFLASHIYGKILSAKFE"
     gene            10042..12699
                     /locus_tag="U2K71_001940"
     CDS             10042..12699
                     /locus_tag="U2K71_001940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM7315993.1"
                     /translation="MEELKERIVNYVIENSEIKNKWGNNTAQKISEKLNVSRNIVSQY
                     LNEFVKDGEFGKINTRPVIFFSLPEKVPNQQIFSSSEEAEKWYDSSFRVGIERVIGSQ
                     GSLRNVIAQIKAAIYYPPNGLPILLHGETGTGKSYLANYVYKDLLANNYIEEKASFIS
                     VNCSEYANNPELFLSNFFGHVKGAYTGAEDGRKGLAELANNGVLFLDEIHALSNECQE
                     KLFQFMDTRKFHRMGDNENWYTSSCWIIFATSEPPEDKLLSTLLRRIPVKLTLPTLDS
                     RSLYERKQLIKIFFKSEEERLAKKILISQQLLDFLLNYRFSENVGNLKNIIQLTCANA
                     FYFLDDLEELYIQMYHLPQWVNFNGMKVMKRQNELKYIQMDDLEKVDEVFNINELIEY
                     LYQTWKKDTDKQQLFEKYDRYFRQKNVDIQNNSALTYPTYQEIRFRQYVKEIERDFQI
                     EFDYYYLQITSIFLNSWIYQIQLTSDDKLYSFINDINSWCSREVQLVNRFIQIGDIYL
                     TKIQKNVLVILFKLMCSKEFQQKRLALILAHGDLTATCIASTANRIVERYIYDAIDMP
                     LMTSSSEIADKINRYLQECTGYEEIILLVDMGSLEKIYLNLELDAQKKFGLLNNVSTR
                     MAIDVANQILLDLPLKQILEQVKENQNVDYIYESSNIKKDMIVCSCASGLGTSFKLKK
                     ILEDSFPRDTDLVIETCDYYSLVNENYLSELRKENNILFIVGTLNPHLEDVEFFSIED
                     MILGMNISKFSNQLNRHFNDNELKVLNNNILKNFSLTNLMEQLTILNPTKLLERVSDA
                     IYILQNDLGISFDNNTCFGLYVHISCLIERLVKQNTLEDEIYFNETSEEFQKFQTHFK
                     QSFSVVEHYYSVDIPIHEVKYVYDYVKRA"
     gene            12771..13247
                     /locus_tag="U2K71_001941"
     CDS             12771..13247
                     /locus_tag="U2K71_001941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7315994.1"
                     /translation="MSIVMSRVDNRLLHGIIVTQWAPMSKANRVMVIDDSVANNDIQK
                     SSMRLARPAGMAVSIITEETAIANFKKGKYDQEKVFVIVKEPETILHLVDNGIRIDRL
                     IVGGTVVKDNALQLSSRAYASDENISTYKKLLDSGVEIVTQFVPADKAQEIQSKLG"
     gene            13261..14049
                     /locus_tag="U2K71_001942"
     CDS             13261..14049
                     /locus_tag="U2K71_001942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7315995.1"
                     /translation="MHFTIFQIIMLTILAFIKHVDYYGIPMIFVNYAVFWGLFTGILM
                     GDIKTGLIVGGTVQLMSLGVAGFGGSSVPDYGTCAIIATAFGSQLGVEAGLAIGLPVG
                     MLGVQLDVIVKILNGFVVRKSQEYANKREFKKMNSVLWLGPIFFGLSAAIPVFIAVTL
                     GQSAVNYVLDVLPNWFMDGLTLAGKMLPAVGIAMLLRFMPTGRYISYLLVGFFLAAFL
                     KVPIIGAAIIGFALAFNVYHRAEKDAQITASAGASDVGFIGEDE"
     gene            14042..14848
                     /locus_tag="U2K71_001943"
     CDS             14042..14848
                     /locus_tag="U2K71_001943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM7315996.1"
                     /translation="MNKMSEKYQVTKKDLRKANYRWLMSVCTFNYQTQQGASVTYALS
                     PILRKLYKDDDEYIEALNNHFQYYNTQPWLAAIILGACVAMEEKQGLEAKDAINDFKV
                     GTMGPIAGIGDSLLMTMIPTIMGSIAAYMALENNPVGIFLWFILIMVIFFFRMRGFEF
                     GYKQGMKIVTEYGEKINYLTEAASVLGLTVVGSLIASVISVSTPLKFTFGEVSMKIQP
                     MLDKILPTLIPVIITAAAYYLLSKKKISMTILILLVIVFAMVMAAFGILA"
     gene            14867..15262
                     /locus_tag="U2K71_001944"
     CDS             14867..15262
                     /locus_tag="U2K71_001944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEM7315997.1"
                     /translation="MRRLIIASHSILAEGFKETLEFLTGMHNQISSLCAYVEEIDDID
                     EQIKELVDTTDEIIVLTDMAGGSVNQKFFTYISDRVHVIAGMNLPLAMSIALQLGNDN
                     LDIGAIIAEAQQQIIYMNNQKSEERIEDE"
     gene            15264..16385
                     /locus_tag="U2K71_001945"
     CDS             15264..16385
                     /locus_tag="U2K71_001945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2961 domain-containing protein"
                     /protein_id="HEM7315998.1"
                     /translation="MNCLNNIWLHKGEESRTISPENKNGEKGKAAMTASHLGIGRKGS
                     PSVPINAGETITLAEMEGPGILRHFWMTVTDKTIAGSFVLRDLILRIYWDDESTPSVE
                     SPLGDFFCNGFGTRCEVNSLPIVVNPTSGMNCYFEMPFRKKAKITLTNEHPEFIKHIF
                     YTFNYALTEVPDDAMYFHAKWNREPITTKGKDYTLIDGIKGTGYYVGTYFALCALERY
                     WWGEGEFKFYIDGDEDFPTITSTGAEDYFGGAWAFHKKNFGERAYAQNFNTLFMGYPF
                     QSKRDHTRDYFSAGKPNPNPVHGFGDDALPMHGLYRWHLPDPISFKENLRVTFQTLGN
                     DDIKLYERQDDISSVAYWYQKEPHEFFEKLLDREERIPR"
     gene            16405..17274
                     /locus_tag="U2K71_001946"
     CDS             16405..17274
                     /locus_tag="U2K71_001946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM7315999.1"
                     /translation="MYGSIEAGGTKFVCAIGNDDLKVLERVSFPTTTPNETMSLVIDF
                     FNHYKEQLESIGVGSFGPIDIHRESKTYGHITSTPKTAWKNFDFVGTLNKHFEIPIAW
                     TTDVNAACYGEYVSGHGKGLSSVVYYTVGTGIGGGAIQDGIFVEGFSHPEMGHTLVKR
                     HSGDTFSGTCPFHHDCLEGLASGPAIEMRTGTKGQDLSIEDPFWEIEADYIAQCACNT
                     TLMLSPDIIIFGGGVMQQEHLKKKVQRRFLELINGYVDTPNIEEYIVTPKLADNAGTI
                     GCLTLAKDVRINS"
     gene            17743..18057
                     /locus_tag="U2K71_001947"
     CDS             17743..18057
                     /locus_tag="U2K71_001947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006148869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM7316000.1"
                     /translation="MELKFVIPNMEKTFGNLEFAGEDKVVQRRINGRLTVLSRSYNLY
                     SDVQRADDIVVVLPAEAGEKHFGFEERVKLVNPRITAEGYKIGTRGFTNYLLHADDMI
                     KE"
     gene            18073..18459
                     /locus_tag="U2K71_001948"
     CDS             18073..18459
                     /locus_tag="U2K71_001948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000985015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM7316001.1"
                     /translation="MMRLANGIVLDKDTTFGELKFSALRREVRIQNEDGSVSDEIKER
                     TYDLKSKGQGRMIQVSIPASVPLKEFDYNARVELINPIADTVATATYQGADVDWYIKA
                     DDIVLTKDSSSFKAQPQAKKEPTQDK"
     gene            18488..19873
                     /locus_tag="U2K71_001949"
     CDS             18488..19873
                     /locus_tag="U2K71_001949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000813487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HEM7316002.1"
                     /translation="MKQRGKRIRPSGKDLVFHFTIASLLPVFLLVVGLFHVKTIQQIN
                     WQDFNLSQADKIDIPYLIISFSVAILICLLVAFVFKRVRYDTVKQLYHRQKLAKMILE
                     NKWYESEQVKTEGFFKDSAGRTKEKITYFPKMYYRLKNGLIQIRVEITLGKYQDQLLH
                     LEKKLESGLYCELTDKELKDSYVEYTLLYDTIASRISIDEVEAKDGKLRLMKNVWWEY
                     DKLPHMLIAGGTGGGKTYFILTLIEALLHTDSKLYILDPKNADLADLGSVMANVYYRK
                     EDLLSCIETFYEEMMKRSEEMKQMKNYKTGKNYAYLGLPAHFLIFDEYVAFMEMLGTK
                     ENTAVMNKLKQIVMLGRQAGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSEMGYG
                     MMFGSDVQKDFFLKRIKGRGYVDVGTSVISEFYTPLVPKGYDFLEEIKKLSNSRQSTQ
                     ATCEAEVAGVD"
     gene            19876..20029
                     /locus_tag="U2K71_001950"
                     /pseudo
     CDS             19876..20029
                     /locus_tag="U2K71_001950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000879507.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
     gene            20052..21257
                     /locus_tag="U2K71_001951"
     CDS             20052..21257
                     /locus_tag="U2K71_001951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000398284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation factor domain-containing
                     protein"
                     /protein_id="HEM7316003.1"
                     /translation="MEGFLLNEQTWLQHLKEKRLAYGLSQNRLAVATGITRQYLSDIE
                     TGKVKPSEDLQQSLWEALERFNPDAPLEMLFDYVRIRFPTTDVQQVVENILQLKLSYF
                     LHEDYGFYSYSEHYALGDIFVLCSHELDKGVLVELKGRGCRQFESYLLAQQRSWYEFF
                     MDVLVAGGVMKRLDLAINDKTGILNIPVLTEKCQQEECISVFRSFKSYRSGELVRKEE
                     KECMGNTLYIGSLQSEVYFCIYEKDYEQYKKNDIPIEDAEVKNRFEIRLKNERAYYAV
                     RDLLVYDNPEHTAFKIINRYIRFVDKDDSKPRSDWKLNEEWAWFIGNNRERLKLTTKP
                     EPYSFQRTLNWLSHQVAPTLKVAIKLDEINQTQVVKDILDHAKLTDRHKQILKQQSVK
                     EQDVITTKK"
BASE COUNT         7375 a         3243 c         4300 g         6401 t
ORIGIN      
        1 cttgaagaac agatatactt tatggatcgt ttgtcttgaa cgattcaagc aatgggcagt
       61 tttagcaaca gattgattaa ttttgaaata agactctatt attacaagtt catccgttgt
      121 aagatgggta taggtcattt gtactcactc cttataattc tttggtcgga actattttga
      181 gtgtagcaca aatgactttt ttagttgtct agcttaattt tacaatcggc gtaaagtaaa
      241 aatgcgaaat taagtagatg agagtgatga tgttcgaaat atctattatt tttaataaga
      301 atacataagc tatctagttt gtaaacaatg agagaaaaaa catcaatatt ttttatcaaa
      361 ggtatttaaa atttcttcta atgttataga atttagttat attaaatttt agcggtagga
      421 acggtaagga ggtgaaaaca ttaagaatta agggctttta gtttattctt tctctccctg
      481 tctaccggtg aaaacattta atataaaaaa aggataaagt cgataaaggc ggactcaatc
      541 gatattaccg gtagagagac cagttttttt taagaataaa gtgtgaattg acgtatcttt
      601 tattcttttt tctagtctac cggtaaattt tgagaggagc gtcatatgaa atttattatg
      661 aataaggaga tgtattaatg gctacaaatt acgcaaaaaa gataaatttt gatgtggata
      721 aactttataa aagtttgata gttgttccca aaaataaatc agaagatgga aattatgaat
      781 tagaagtttt gcaatttaag attgaacaaa agtccagtga aagatttatg agcaatcaat
      841 tagtgaaaat caaaaaatgg ctccctatga ttgatcaatt agtatctaat aataaggaag
      901 ctaaaaattt atgtattttt gtcttattat tacatggaag agttattaac ttagttaatc
      961 gaaaaagtac gactaaattt gcaaactcat tctggttttt agaaaatatt aatgaaacag
     1021 tggaaaagaa tggagtttat aaaggtgatt ttcattttca aaatgctgag actattgaac
     1081 gagaacatca ttatttcgat ttattagtga atacagtggt agaattagcg aaatgggcaa
     1141 agtcaaacga aacttatagt caagtactta aaagaattat gagtcaagaa acttcattta
     1201 tctatgagtt aagcaagtca aatttgaaat atacttataa tgaagatgaa cttgaaattt
     1261 tagataatcc agaaaattca caatggatta aagagttgaa gagagaagac tttattgaga
     1321 cactaagaat tctaaaatcg ttatttgatt tgaaattagc caaagctacc atcatgggaa
     1381 ttgaaagaga ttcatattat tgggaggaaa ttggactata tgctccccag attgaaggtg
     1441 aagataaaga gaaaaggata gacaacatta ttaactatat taatagttta agtgaacctt
     1501 taaaatgtag aggacttgat tttataggaa cattaggatg gacagaaaca tactatgaga
     1561 aaatatataa agaaataatt gaacaatatt ttcctaaata atttaataag gattgattta
     1621 ttgtaaaaga aaaaagttgt gattttggaa atcacaactt ttttcttttg tcaagatttt
     1681 gagtgggata tatttttgta gaactgaaga tcatgaaatt atgagaggtt ggaggtaatg
     1741 tagtcataca aataaaaatt ttttagaaag tgagtggtaa ttaatgaaaa tgaatcacac
     1801 ttcaattcag aagcatttac aactgttaaa tacactgggg atttcttatg ttaatttgag
     1861 aactttttct acattaaaca agaaaaccgt ttctcatatt ttttctactg cagattctca
     1921 agcaattatt aactatttag aaaaacagaa attgtctgaa ggtacagtaa acataactta
     1981 taatcctatt aaacaagagg tgattacaca aaaaaagcat tctgtgagag acaatgatat
     2041 tgaaaaaatt cggtttgtat ttattgatat tgaccctaaa aggaaagagg gaagtgcaac
     2101 agatagcgaa aagaaaaagg cagaaaatgt tatggaacaa gtagaacgtt atttgaaaga
     2161 aaagagaata gaatcatttg taaaagttga tagtgggaat ggctaccata tttttcttcc
     2221 cataaatgaa caaccaaata atcacgagac gattcttact attcagaatt ttcttcaatt
     2281 acttcaccga agatttggag tagaagatga agttgatatt gatacttctg tttataatcc
     2341 ttcaagatta tgcaaattag ttggtacacc agcggtcaaa ggaaagtcca caaaagagcg
     2401 tccgcatcgt ttatctagtt tcctttctat tcccgataac atagatagaa gtaattgttt
     2461 taaaattctt gaacagatag ttgaagaaaa ttccatgatg tcaacctcta gagtaaaaag
     2521 ccatccatct tttagagaag tgttaccttt tattcaagca gactataaac aatggttagc
     2581 tcactatcct gaattaactt ataccataaa agagggcgat cataaaggag ttaccttact
     2641 tatatttgac gaatgtccat taagaaaaca cacgaataat tctaatggtt cttgtttgtc
     2701 tgttacggct gataataaga ttcgatttca ttgtctgcat gcttcggata aaaataaaga
     2761 tattcaagac tttgtaaaaa aatatccgtt gccaaaacct gtaaatcctt ctgtagaatc
     2821 attagaatcc aatcaagaat attattttga ccagtttaaa ctgaatcaag aaggagtatt
     2881 tttggtgaaa gaaggtcaag aagaaaaagt tgcttgtccg atgtttatta agcaaatacg
     2941 caaaaatatc gaacaaaaag tcattacact tgttcttgcc tattatgtcg atcattgttg
     3001 ggatgaaata gaaatagaag gtggatgttt acaaactaat aattttaaac aattagcaaa
     3061 acatggctta atcattaaag ctagaagaga agctgaagtc gttgactttt tacagattca
     3121 acgggagaat gctccaatct tttttgaaca tgaatctctt ggctggaaaa aagaaaatga
     3181 agcaatgatt tttcagttac aagattcttt tggacgtgaa attccttctg tattaactaa
     3241 aaactcatgc taccaattaa caactaatgg tacagaagcg gaatttgacg cattggttca
     3301 taagtatgtt ttaggaacga acttagaact tgcttggtcg attggtattg cttctattgt
     3361 gttaggctat ttaaacgtta cgaaggctaa tacttttttg agtttactaa ttaacctaca
     3421 gggcaagagc acaacgggta aaaccactac gttacacttt attgcctctt tatttggaaa
     3481 tccaactacg gttcttagaa atatgaatgc taccaataaa gccattataa agctagcatc
     3541 aaacaatcag ggaggtgttc cactaatact agatgagtta ggagcagcaa ctcaaaataa
     3601 ctttagctcg ttgatctatc aattagcttc aggagaagaa aggctaagat tagattctaa
     3661 ttgtcagctt atgccacaag agactttttc tgttttgacc cttacctctt ctgaagagcg
     3721 tttggaaaat tatttcaatg agaaattaac tggtttgaaa gttcgatata tcgaattttc
     3781 tgatatcaag tttacaaagg atgcgaatca cgcagagaaa gttaaaagtc tttcttctaa
     3841 tacttttggt tggggtataa agttatttct tttgaaactc tttgaacaag ggattgcaaa
     3901 aattgaagca acttttgtcg aagctcaaaa aaaaatatgg gctgttttac ctgaacattc
     3961 tttgaagcaa cgcattggta ataatctagc tttgatttat agtggagctc tcctaattca
     4021 agatatactg ggctatcctt tacgattaga gcatatacag acggtgctta ttaattcgta
     4081 cgaacagtta attgtagata ctagtttagg aagtgagcaa acggtatttg accgagtgaa
     4141 aacgttgttg gtgatgaata gccataaatt tttaacaaac agtgacaaca aacatacgcc
     4201 cagtacgatt tggggaaaag tatcaattca aaaaaatggg gatattaagg taaatatttt
     4261 tcctatcccg tttgaggact tgttaaggcg agaatttcaa gtaaaggatc taacctatct
     4321 ttttagacaa ttgcttgatt cgggtaatat gtgtgcagaa aaagggagaa gaacgaaacg
     4381 tattcacatg aatcgtcaag ttcttccaac atacgagatt ctattacctt cttcactcaa
     4441 atcgtatttt cgattgtaat ttagaataaa ccaaagtatg aaagtgaggc accatgttat
     4501 ggaaatattt cttattgatt tagcaaaaca actgattact tatctgtatg atgacgagca
     4561 aacccaaacg gaagtaaatg aagcttgatc tatgcttcat ttacttcatt caatcaaaca
     4621 tttaagaaag gggaattcta atgactaaaa ataaaatgac actaaaagca gaggttcttc
     4681 tgtatattca agaacatttt tctaatcaag ccttttttac aaaaccaatt tacttggctt
     4741 tcgaaataag aggggtatct gctggatcta ttggaggtac tcttcaagcg ttgaagaacg
     4801 aaggatattt agaaaatcac tttgtgcaac gttcgttcaa tggaagagtc gcgaaagaat
     4861 ggtatttagt tcattcatag agaggtgatt caagcatgcg tgtggcttgt tatgatcgaa
     4921 cgtctgttta tgaagaagcg aaaatcagta atgacgctca atatgatgca gtgaaagagt
     4981 atgttttaga gaaatatgaa caaattcctc aacgattttc agacaaagga gaatcaggtg
     5041 gaaaagcgga tcgtccagct tttcgacagc ttagagcttg ggtttgtgcg tcagaagaag
     5101 agggggtgat ctatgtctgg cgttatgatc gattggcacg tcaagccaaa atagccttag
     5161 aatttattga gttgtgccaa caacatcagg ttcaagttta ttcgatttct gaacctcttc
     5221 ctgcttcttt ctcgcacgcg tcaacgaata aactatttgt gcaattattg ttcacttttg
     5281 cagaacaaca acgaaacgtc attattgaaa atgttcgtgc cggacttgcc tataaaaagc
     5341 aacaacggca atatttgtct tctgcggttc catatgggta tcgtttagtg aatgggaaaa
     5401 tccaacaaga agcacatgaa gcaaaagttg tccgccatat ctttcaactt tatttgacta
     5461 aaaaatatgg ctacaaaaag ctttgtcaac gattgacgca acaaaagttc ttcttccgtg
     5521 aacgtccttt tcagccctat catatttata gtatcctaaa aaatccattg tactatggcg
     5581 aaatcaaagg aggatctttg gggaagtatc taggaacctt tgaaccgatt ctctcgaaaa
     5641 caatcttttt acaagcccaa gagattcgcc aatcaaggtg tacagctaaa aaagatacgt
     5701 atccgtactt attacgtcaa aaaattcggt gtcccttctg tggtcgtcat ctgagttcga
     5761 agtatcaatg gaatacaaag aagacgaaga cgcttcatta ttatcattgt accgaccgaa
     5821 gctgccaagg aatctttctt cgggcagaaa gggttgagtc agaagtattg gaaaagatac
     5881 aggctttttt tcaatacgaa ggcgtctatc aagccttgat tgcccaattg acgcaacaat
     5941 tagccgaaaa gcgaaaagat gaaaaacaca aagtaatcca acaaaagaaa caagaagcac
     6001 aatggctcaa ggcatttgaa gagggcaatt gttcactgga agaactcaag caacagctgc
     6061 aagctcttca aaagccgagg tctacttctt cgtttctttc actccaacag tgtgaagcaa
     6121 aagtgaaaga atggcttcaa ctaaaagacc tctctagtcg acaattgtta tgtgaccatg
     6181 tttgtcggat cgaggtaaca aaggaaaaaa cagtctgtgg gatttacttc cgacaaatga
     6241 cacaaaatat tttagaaggg aacgaatgat ctatggaaac gacaccaacc aaacgagtcg
     6301 cattgtatat tcgggtgagt acccaagaac aagctttaga aggacatagt attcaagcac
     6361 aagaacaaaa tggacgacag tttgccgaac ggatgagcta tgaagtggta gaagtttatg
     6421 ccgatgaagg gattagtggg aaatccacaa aaaatcggtt cgcctatcaa cgaatgatga
     6481 acgatgctcg gaaccatcgt tttgatctac tagtgatttg gaaattgacc cgtctcggac
     6541 gcaatatgtt agatgtctta caaaccgtcg aggaactgtt gcgatatgat gtggggttac
     6601 attcgatctc ggaacaattt gacgtgacta cttcgacagg aaaactaatg ttacaactcc
     6661 ttgggagttt cggtgaattt gaacgcaacc aaattgcgga gaatgttcag atgtcaatgc
     6721 aaagcttagt gaaagataaa aagcgttatg caggtggacg acgtttaggc tatgtcagtg
     6781 gtattgacac gaatggtcaa aaacaattgg tcattgaacc agaagaggcg aaaattgttc
     6841 gtctgatcta tgcaaaatat ttaggcggag cagggtatcg agcgattgcc aattttttga
     6901 atcgtcaagg atatcaaaca gtcaaaaaga atgctttttc taccacggca gtcaaagata
     6961 tcttacgcaa taaaatttat gcagggtatt tagaatatgc acgctatatc aattgggaaa
     7021 ccaaacgacg aaagggcaag aacccaaaac cgattttagt gaaaggtacc catgaaccga
     7081 ttatttcgga agaagattat caaaaagtgc aagaacgcct tgttttagaa ggcaaacagc
     7141 ccaaatggaa ccatacaggt gaaaatgtat tgacaggctt gttacgttgt ccagaatgtg
     7201 gggcaccgat ggcagcgagt aatgtcacaa atactctaaa ggatggcaca aagaaacgaa
     7261 ttcggtatta ttcttgttca gtctttcgga ataaaggagc aagtgtgtgt catgccaatt
     7321 cgattcgtgc cgaacaagcg gaaacatttg tcgccgaccg attaaaggag atcgttcagc
     7381 taccagaagt tcttccccga ttggtggcag cgttaaatga agaaatagtg cgacaaagcc
     7441 aaccattgga acaagaactc gtggtgttac tggaacgaaa ggaagaattg aagactaaaa
     7501 ttgaaaaatg ggaagctgct ttggaagata gtcctgaatt gtttccgatg ttaaaagacc
     7561 gcttagacga attgactgaa aaacgacgac agttgcacat tcgagaaaat gaaatcttag
     7621 ggatctttca acaacaagga gagccgattc aagtcaaaga tgtccaacgg atattgacta
     7681 gtttggatcg ttttcttgcc cagagtgaga agaagcaagt caaagccttg tatcgtacct
     7741 ttattgagaa aataaccttt gatcctaact ataaggagat attggagatt acaatgaaat
     7801 tcacgcctgc cgtggtacaa caattgaatg agcaatatca aatagccgta tccaaaacaa
     7861 aggatacggc tatttttgtg ttaaagacac cctttacgtt aacgatctag tctaggttat
     7921 atcaaacaac gttagagggg cgctaacgct tctctagcga tttgaaatcg ttagatgagt
     7981 cgttagggag gtccttatca taaataggga tatttattaa tgaatatttg aaatataatt
     8041 gtatataaga attaaaaaga gtggggatag atatgaaaaa gatgggtaag tgtattttat
     8101 gtgaaaaata cactgaactt actaaagaac atgtaccacc taaaaaatca gggaatactg
     8161 gaggtaaaaa gaccagaact ggtaatttag atgatttcct taagagcgat tttacaaaag
     8221 gtgattttcc gaaggggatt aaaaggaaac ctcaaggaaa tgtttattat acgttatgta
     8281 gtaaatgtaa tagttttttt ggtagtgagt atgttgagga atatattaga tttgcagaag
     8341 acaataaaaa tttcctttat aacaatacaa gtttaaagaa tggtaagtct gatttaacac
     8401 attcaataaa gaaaatgaat tcacttagag ttgcaaaagc aattgtagca atgtttttta
     8461 gtttaaatgg ggatgaagat tcaatggata agccattctt agattcggta cgagaatatc
     8521 ttagcaatcc taaaagtact ttatttcctc atgaagacta caaaattata atgaattatt
     8581 atcttccaat aggtgctcca cagaattcta aaatcaaaat tcctaatgac attgtaaatg
     8641 atggtgacac tcacaatgta ataaaaggtt ttatgcccct tgatctatca actgggagaa
     8701 aaattcgatt ttcagaaatt cagtatgaag gaattggttt tactcttata gatttaaaaa
     8761 attcaacttt tgaaataaaa gaaggattag atttaaaaaa tctcctttgt gcagatgata
     8821 aaatcaaaaa aatacaattg tacaaagtcc ctatattaac accaacagta cttacaatgt
     8881 tggagatgga aaataatatt aattatattg actcaggttc agatttgctg attaggtggc
     8941 aggtaataac taattttctt atggaattaa aaaaatacag taatactcaa acgaagttct
     9001 cttgaagtta taaagaatac aataaatata aatttccttt agactatatt aaaatcagca
     9061 atcgaaggaa aataaacgaa gaaaatggta cgcaaaatct aactattaga gcaaaaacag
     9121 gaagctgctt tggaagatag tcccgaattg tttccgatgt taaaagatcg tttaaatgaa
     9181 ttgactgaaa aacgaagaca gttacaaatt cgagagaatg aaatcttagg gatctttcaa
     9241 caacaagggg aaccgattca agtcaaagat gtccaacaga tattgactag tttggattgc
     9301 tttcttatcc agagtgagaa gaagcaagtt aaagctttgt atcacacctt tattgagaaa
     9361 atcacctttg atcctaatca gaagtagata ttgccgatcc cgatgagatt cacacctact
     9421 gtgatttgat aattgaatga gtaatatcaa atagccgtat ccaaaccaaa ggatatggct
     9481 atttttgtgt tagggatata cgtcacgtag cttgttatca agaataagtt tctttatttt
     9541 tttgcttttt ctaaaactga gtatgctaaa ataagagggt atatagcaac tttaaataag
     9601 agataggagt gtatcttttt gggtgcagaa ttaaatcaga agttatttag tgcggcagat
     9661 aatctccgta gtaaaatgga tgcgtcagaa tataaaaatt atttattggg gctgattttc
     9721 tataaatatt tatccgaccg tttgttggaa caagtggttc ttttagcaga tgaatcctta
     9781 gaggaatatg acacagtaag caaacaaacg atgttgtatc gtgaattact ttcagatgaa
     9841 gaatcaaaag aagatctgat tgcaacaatc gttgatattt tggggtatgc gatttcgcca
     9901 gagtatcttt tcaacgtctt agcggatcaa gcaaaacaag caacgttcca attgaatgat
     9961 ctaaacaaag cctttttagc aagtcatatt tatggaaaaa tactgtcggc aaaattcgaa
    10021 tagtgtcgag gaggtgctgt gatggaagaa ttaaaagaac gcattgtcaa ttatgtcata
    10081 gagaactctg agattaaaaa taaatggggg aacaatacag cacagaaaat atctgaaaaa
    10141 ttaaatgtta gtcgtaatat tgtctctcaa tatttgaatg aattcgtaaa agatggagaa
    10201 tttggaaaaa tcaatactcg tccagtaatc ttcttttctt taccggagaa agttccaaat
    10261 caacaaattt ttagctcttc agaggaagcg gaaaaatggt atgatagctc ttttagggtt
    10321 ggaatagaaa gggttatcgg aagtcaagga agtctgcgaa atgtaattgc tcaaattaaa
    10381 gcagcaatct actatccgcc aaatggctta cccattttac ttcatggcga gactggaact
    10441 ggaaaaagct atttagcaaa ttatgtgtat aaggatttgt tggccaataa ttatatagaa
    10501 gagaaggcat ctttcatttc agttaattgt agtgaatatg ccaataatcc agaacttttc
    10561 ttgtccaatt tttttgggca tgtaaaaggt gcatatacag gagccgaaga tggaagaaag
    10621 ggtttagcag aattagctaa taatggagtc ttatttttag atgaaattca tgcattgagc
    10681 aatgaatgtc aggagaaact ttttcagttt atggatacga ggaaatttca tcggatgggt
    10741 gataatgaaa actggtacac atcaagttgc tggattatat ttgcaacatc agaaccacct
    10801 gaggataaac tattatcaac tttgttgaga agaatacctg taaagctaac acttccaaca
    10861 ttagacagtc gttctcttta tgaaagaaaa cagttaatca agatattttt taaatcagaa
    10921 gaagaaagac tagcaaagaa aatactgata tctcaacaat tattagattt tttattaaac
    10981 tatcgcttta gtgaaaatgt tggcaatttg aaaaatatca ttcaattgac ctgtgccaat
    11041 gccttttatt tcttagatga tttggaagaa ctttatattc agatgtatca tttaccacaa
    11101 tgggtgaatt tcaacggcat gaaggttatg aaaagacaga atgaattaaa atatattcaa
    11161 atggatgatt tggaaaaagt agacgaagtt tttaatatca acgaacttat agagtatctt
    11221 tatcaaacgt ggaagaaaga cactgataag cagcagcttt ttgagaaata tgatagatat
    11281 tttagacaaa agaatgttga tatccaaaat aattcagctt taacatatcc cacctatcaa
    11341 gagattcgat ttagacagta tgttaaggaa atagagagag attttcaaat agaatttgac
    11401 tattattatc ttcaaataac gtctatcttt ttgaattctt ggatttatca aatccaatta
    11461 acatcggacg ataaattata ttccttcatc aatgatatta atagttggtg tagtcgagaa
    11521 gttcaattag tcaatcgttt tattcaaata ggggatattt atttaacaaa aattcaaaaa
    11581 aatgttttgg ttattttatt taagttaatg tgtagtaaag aatttcagca aaaacgttta
    11641 gcactgattt tggctcatgg agatctaacg gcaacctgta tagcaagtac tgctaatagg
    11701 atcgttgaga gatatatata cgacgccatt gatatgccac taatgacttc aagtagtgag
    11761 attgcagaca aaattaatag atacttacaa gagtgtaccg gatatgaaga aattatttta
    11821 cttgttgata tgggttcact ggagaaaatt tatctaaatt tggaactaga cgctcaaaaa
    11881 aaattcggac tactaaacaa tgtttcaaca agaatggcta ttgatgttgc aaatcagata
    11941 ttattagatt taccgcttaa acagattctt gaacaagtaa aagaaaatca aaatgtggat
    12001 tatatttatg aatcatcaaa tattaagaag gatatgattg tctgttcttg tgcaagcgga
    12061 ttaggaactt cgtttaaatt aaaaaagata cttgaagata gctttccaag ggatactgat
    12121 cttgtcatag aaacgtgtga ctattactct ttagtgaatg aaaactattt atcggagctt
    12181 cgtaaagaga acaatattct ttttattgtt ggaacactaa atccacatct agaagatgta
    12241 gaattctttt caattgaaga tatgatttta ggaatgaata taagtaaatt ctccaatcaa
    12301 ttgaatagac actttaatga taatgaactg aaagttttga acaataatat tctgaagaat
    12361 tttagtctaa cgaatttaat ggagcaattg accatattaa atccgactaa attattagaa
    12421 cgggtttcag atgctatata catcttacaa aatgatctag gaatttcatt tgataataat
    12481 acgtgtttcg gattatatgt tcacattagt tgcttaattg aacgattagt aaaacaaaat
    12541 actttggaag atgaaattta tttcaatgaa acgagtgagg aatttcaaaa atttcagaca
    12601 cattttaagc aatctttcag tgtcgttgaa cattattata gtgtcgacat cccgattcat
    12661 gaggttaaat acgtttatga ttatgtaaaa agagcttgat ttatcaagtt ctttttgttt
    12721 tggcatggta attgcttgta agaaaatgaa agataaagga ggaagtattt atgagtattg
    12781 ttatgtcgag agttgataac cgtttattgc atgggattat tgtcacgcaa tgggcaccaa
    12841 tgtctaaagc aaacagagtg atggtgatcg atgattctgt cgcaaataac gacatccaaa
    12901 aatcaagtat gcgtttagct agacctgctg gaatggctgt atcaattatc actgaagaaa
    12961 cagcgattgc gaattttaaa aaggggaaat acgatcagga aaaagtattt gtcatcgtga
    13021 aagagccgga aacaatcctt catttagtag ataacggtat tagaattgac cgactaattg
    13081 ttggcggtac tgttgttaaa gataatgcat tacagctatc ttctagagcc tatgcatcag
    13141 atgaaaatat cagcacgtat aaaaaattgt tggattctgg tgtagaaatt gttacacaat
    13201 ttgttccggc ggataaggca caggaaattc aatcaaaatt aggataaagg agtaaggata
    13261 atgcatttta ctatttttca aattattatg ctgactatat tagcatttat taaacatgtt
    13321 gattattatg ggattccgat gatctttgta aattacgcag ttttttgggg attattcaca
    13381 ggaattttga tgggtgatat aaaaacaggt ttgatagttg gcggtacagt tcaattgatg
    13441 tcattaggag ttgccggttt tggtggctca tctgtaccgg actatggaac gtgcgcaatt
    13501 attgcaacgg catttggtag ccagcttgga gtagaagccg gattagcaat aggactacct
    13561 gtaggtatgt taggggtgca actagatgta attgtcaaaa tcttaaatgg ttttgttgtt
    13621 cgcaaatcac aggaatatgc gaataaacga gaatttaaga aaatgaatag tgttttgtgg
    13681 ttggggccaa ttttctttgg tttatctgca gcaattccag tatttattgc cgttacttta
    13741 ggacagtctg ctgttaatta cgttttggat gttttaccta attggtttat ggatgggcta
    13801 acattagccg gaaaaatgtt gcctgctgtt ggtattgcga tgttgttacg ctttatgcct
    13861 acaggtcgat acattagtta tttacttgtt ggatttttct tagccgcttt tttgaaggta
    13921 cctattattg gagcggcgat cataggattc gctttagcat ttaatgtgta tcatagagct
    13981 gaaaaagatg cacaaatcac tgcttctgcg ggagcttctg atgttgggtt tattggggag
    14041 gatgaataaa atgagcgaaa aatatcaagt aacgaaaaag gatcttagga aagcgaacta
    14101 tcgttggttg atgtcagttt gtacctttaa ttatcaaaca cagcaaggtg catctgtgac
    14161 ttacgcattg tcacctattt taagaaaatt atacaaagat gatgatgaat acatcgaagc
    14221 tttaaataat cactttcagt attacaatac acagccttgg ctggcagcca ttattttagg
    14281 tgcttgtgtt gcaatggagg aaaagcaagg attagaagca aaagatgcga ttaatgactt
    14341 taaagttggt acgatgggtc caattgctgg tattggggac tctcttttga tgacgatgat
    14401 cccaaccatc atgggatcaa ttgcggctta tatggcttta gaaaataatc ctgtaggaat
    14461 ctttttatgg ttcattttaa tcatggtaat cttctttttc cgaatgagag gctttgaatt
    14521 tggttataag cagggaatga agattgttac tgaatacggt gaaaagatta attatttaac
    14581 tgaagctgct tcagtattgg ggctaacagt agttggatca ttaattgctt cagttatcag
    14641 tgtttcaacg ccacttaaat tcacttttgg tgaagttagt atgaaaatcc aaccaatgtt
    14701 ggacaaaatt ttaccaacat taattccagt aattattact gctgcagcat attatctact
    14761 cagtaagaaa aaaatcagta tgaccatttt gattctactc gttattgttt ttgcgatggt
    14821 tatggctgca tttggaatat tagcgtaaag gaaaaggatt tgaattatga gaagattgat
    14881 cattgctagc cattcaatac tagctgaggg ttttaaagag acactagagt ttttaacagg
    14941 tatgcataat caaattagta gcttatgtgc atatgtcgaa gaaattgatg atatagatga
    15001 acaaattaaa gagttagtgg atactacaga cgaaataatt gttttaactg acatggctgg
    15061 aggaagtgta aatcaaaagt tttttaccta tatttctgac agagtgcacg taattgcagg
    15121 aatgaatctt cccttggcaa tgtcaatcgc tttgcagtta ggaaatgata atttagatat
    15181 tggagcaatt attgcggagg cacaacaaca aatcatctat atgaataatc aaaaaagtga
    15241 agaaaggata gaggacgaat aaaatgaatt gtttaaataa tatatggctc cacaagggag
    15301 aggaaagtag aactatttca ccagaaaata aaaatggtga aaaagggaaa gcggcaatga
    15361 cagcgagtca tctaggaatt gggagaaaag ggtcaccttc tgttccgata aatgctggag
    15421 agacaattac tttagcagaa atggaaggcc ctggaatttt acgtcatttt tggatgacag
    15481 tgacagacaa aacaatcgct gggagtttcg tcttacgtga tttaattttg cgtatttatt
    15541 gggatgatga atccactcct tctgtagaaa gtccattagg agattttttt tgtaatgggt
    15601 ttgggacaag atgcgaagtg aattccttac caatcgtagt gaatccaaca tcagggatga
    15661 attgctattt tgagatgcca tttcgaaaaa aagctaagat tactttaacg aatgaacatc
    15721 ctgagtttat taagcacatt ttttatacat ttaattatgc attgactgaa gtaccggatg
    15781 atgcgatgta ttttcatgct aaatggaacc gtgaacccat cacgacaaaa ggaaaagatt
    15841 acacccttat tgatggaatc aaaggcactg ggtattatgt aggaacctac tttgctttgt
    15901 gcgcattgga aagatactgg tggggggaag gagaatttaa gttttatata gatggagatg
    15961 aagatttccc tacaataaca tctacaggag cagaagatta ttttggcggc gcttgggctt
    16021 tccataaaaa gaactttgga gaaagggcat atgctcaaaa ttttaatacg ctatttatgg
    16081 gctatccttt tcaatctaaa agagatcaca cccgggatta ttttagtgct ggaaaaccta
    16141 atccaaatcc agttcatggt tttggcgatg atgctttacc gatgcatggg ttgtaccgct
    16201 ggcatttacc cgaccccatt agtttcaaag aaaatttacg tgttactttc caaaccctag
    16261 gaaatgatga catcaagctt tatgagcgtc aagacgatat ttcttctgtt gcctattggt
    16321 accaaaagga accacatgag tttttcgaaa aactattaga cagggaagaa cgaattccta
    16381 gataaaagga cggtggagta agaaatgtat ggctcaatag aagcaggtgg gactaaattt
    16441 gtttgtgcca ttggcaatga tgatttaaag gttttagaaa gagtgagttt tcctacaact
    16501 acaccgaatg aaacaatgag tttggtaatt gatttcttta atcactataa agaacaatta
    16561 gaaagcattg gagtagggtc atttggaccg attgatatcc atcgtgaatc aaagacctat
    16621 ggacatatca cctctactcc aaaaactgcc tggaaaaatt ttgattttgt tggaacgttg
    16681 aataagcatt tcgaaattcc aattgcctgg acaaccgatg tgaatgctgc ttgttacggg
    16741 gagtatgttt caggtcatgg aaaaggttta tccagtgttg tttattacac tgttggaact
    16801 ggaattggtg gaggagctat acaagatggg atttttgttg aagggtttag ccatccagaa
    16861 atggggcata cgttagttaa gagacattca ggagatactt tctctggtac ttgtcctttt
    16921 catcatgatt gtctcgaagg gttggcatct ggacccgcta tcgagatgcg tacgggaacc
    16981 aagggacaag atctttctat agaagatccg ttctgggaaa tcgaggcgga ttatattgca
    17041 caatgtgcct gtaatacgac cctgatgctt tcaccagata ttataatttt tggtggtggt
    17101 gtcatgcaac aagaacatct gaaaaagaaa gtacaaagac gttttttaga actaattaac
    17161 ggctatgtcg atacgccaaa tatcgaagaa tacattgtca cacctaagtt agcagataat
    17221 gcaggaacta taggatgctt aactttagca aaagatgtta gaatcaattc ttaagcaaaa
    17281 aagtattagt caataaaatg cttatgaagt ccgatcaact taatgtggtc ggactatttg
    17341 tattcagcaa tatcattcac tcatgatacc cagctccata ccacaaataa tgatattttt
    17401 taagagaaaa atagcataaa aatctagtta tccgcataaa aactggactt atcacacttt
    17461 atcaaggtca aaaccactca atttactact aatttactac ttatgaatga gctttgatac
    17521 gacgatttat ccttgaaaag tgaagatata aagatacttc caataaaatt tgaatattta
    17581 ataggtagac acttcaaaaa atgaggtgtc tattttttca cccgattttg aaaggaagtg
    17641 aacttatgaa aacaaaaaat caagaatcaa aaggtcgttc cccactcttt aagaccatca
    17701 aacattcatt cagccaataa aaaagaaagg ataggtaaaa atatggaact taaatttgtg
    17761 attcccaaca tggaaaaaac attcggcaat ttagaatttg ctggcgagga taaagtcgtt
    17821 cagcgaagaa tcaacggacg gctaactgtc ttatcaagaa gctataatct ctattctgat
    17881 gttcaaagag cagatgatat tgtggtggtg cttcctgctg aagctggcga aaaacatttc
    17941 ggctttgagg aacgtgtgaa gttagtcaat ccacgtatta ccgcagaggg ctacaaaatc
    18001 ggcactcgtg gttttacaaa ttacctttta catgctgacg acatgataaa agaataaaga
    18061 aagagaggaa aaatgatgag attagcaaat ggcattgtat tagataaaga cacgactttt
    18121 ggagaattga aattctctgc tctacgtcgt gaagtgagaa tccaaaatga agacgggtcg
    18181 gtttcagatg aaatcaagga acgtacctat gacttaaaat ccaaaggaca aggacgcatg
    18241 attcaagtaa gtattcctgc cagcgtgcct ttgaaagagt ttgattataa cgcacgggtg
    18301 gaacttatca atcccattgc ggacaccgtt gctactgcca cctatcaagg agcagatgtt
    18361 gactggtata tcaaggcaga cgatattgtg ctgacaaagg attctagttc attcaaagct
    18421 caaccacaag caaagaaaga accgacacaa gacaaatagt cgctaggtag aaaggagact
    18481 ttttcgcatg aaacagcgtg gtaaaaggat tcgcccatct ggtaaagatt tagtctttca
    18541 ttttacgata gcgtcactcc tgcctgtttt cctgctggtt gtcggactgt ttcatgtgaa
    18601 gacaatccag cagatcaact ggcaggattt taacctatca caagcagata agattgacat
    18661 tccctattta attatcagtt tcagtgtcgc aattcttatc tgcttgctgg tagcgtttgt
    18721 attcaaacgg gttcgctatg atacggttaa acaactttac caccgtcaaa aactggcaaa
    18781 gatgatactt gaaaacaagt ggtatgaatc tgaacaggtc aaaacagagg gtttctttaa
    18841 agatagtgct ggtcgtacaa aggaaaagat aacctacttc cctaaaatgt attatcgact
    18901 taaaaatggc ttgatacaga tacgggtgga aatcacgctg ggaaaatatc aagaccaact
    18961 cttacacttg gaaaagaaat tagagagtgg cttgtactgt gagctgacgg ataaagagtt
    19021 aaaggattcc tatgtggaat atactttgct ctatgacacc atagccagtc gtatttctat
    19081 tgatgaagta gaagctaaag atggtaaact tcgcttaatg aaaaacgtat ggtgggaata
    19141 tgataagctc cctcatatgt tgattgctgg tggtacaggt ggcggtaaaa cttactttat
    19201 actgacactg attgaagcct tgcttcatac agattcaaaa ctgtatattc ttgacccgaa
    19261 aaatgctgac cttgcggact taggttctgt gatggcaaat gtctactata gaaaagaaga
    19321 cttgctttct tgcattgaaa cattctatga agaaatgatg aaacgtagtg aggaaatgaa
    19381 gcagatgaag aactataaga ctggcaaaaa ttatgcttac ttaggtctcc cggcacactt
    19441 cttaatcttt gatgaatacg tcgctttcat ggaaatgctg ggaacaaaag aaaacaccgc
    19501 agttatgaat aagctgaaac agattgtcat gttaggtcgt caagctggct tctttctaat
    19561 actggcttgt caacgtccag acgcaaaata tttaggcgac ggaatccgtg atcagtttaa
    19621 tttcagagtg gctttaggtc gtatgtctga aatgggctat ggcatgatgt ttggcagtga
    19681 cgtacaaaag gatttcttct taaagcgaat caaaggtcgt ggctatgttg atgtaggaac
    19741 aagtgtcata tcagagtttt atactcccct tgtaccaaaa ggatatgatt tcttggagga
    19801 aattaaaaag ttatccaaca gcagacagtc cacgcaggcg acgtgcgaag cggaagtcgc
    19861 aggtgtggac tgatcttgct ggctggtgtg gcaatagcca cgccagcact taacccctcg
    19921 gtatctaaca ggggggtaca aatcgacagg aaacagtcaa aaaaacatta gaaaatcctt
    19981 tggttacaag ggatttacaa aatttcagcg tatgtcaaat gggctttaaa agttgacata
    20041 cgcctttttg attggaggga tttttactga atgaacaaac ttggttacag catttaaaag
    20101 aaaaacgctt ggcttatgga ctatctcaaa accgtttagc tgttgcgact ggtattacaa
    20161 ggcagtatct aagcgatatt gaaacaggaa aagtcaagcc atcagaggat ttacagcagt
    20221 ccctttggga agctctggaa cgcttcaatc ccgacgctcc ccttgaaatg ctgtttgatt
    20281 atgtaagaat tcgctttccg acaacagacg tacagcaggt ggtcgaaaac atcttacaac
    20341 tgaaactgtc ctattttctt catgaggact atggtttcta ttcttattca gagcattatg
    20401 ctttaggcga catattcgtc ctttgctccc atgaactgga caaaggagtt ctggtggaat
    20461 tgaaaggtcg tgggtgcaga caatttgaaa gctatcttct ggcacaacaa agaagctggt
    20521 atgagttctt tatggacgtt ttggtggctg gcggtgtgat gaaacgcctt gaccttgcca
    20581 ttaacgataa gacagggatt ttaaatatcc ctgtactcac tgaaaagtgc caacaggaag
    20641 aatgtatctc cgtcttccgc agttttaaaa gctatcgcag tggcgaactg gtacgcaaag
    20701 aggaaaagga atgtatggga aacaccctct atatcggttc attacaaagt gaagtttatt
    20761 tctgtatcta tgaaaaggac tacgagcagt acaagaaaaa tgatattccc attgaagacg
    20821 cagaagtaaa aaaccgtttt gagattcgat tgaaaaatga gcgtgcctat tatgcagtcc
    20881 gtgatttact cgtctatgac aatccagagc ataccgcctt taaaattatc aatcggtata
    20941 tccgttttgt agataaagac gattccaaac ctcgttctga ttggaaactg aatgaagaat
    21001 gggcttggtt tattgggaac aatcgtgaac gattaaaact aaccacaaaa ccagagcctt
    21061 actccttcca aaggacgctg aactggctat ctcatcaagt tgccccgacc ttaaaggttg
    21121 cgattaaact tgatgaaatc aaccagacgc aggttgtaaa agacattctc gaccatgcga
    21181 aactgacaga ccgacacaag cagattttga agcaacagtc agtaaaagaa caggacgtga
    21241 taacaacaaa aaaataactc aaatacaaat tcattgaata tagagaggag aacattttta
    21301 tgaattttgg acaaaacct
//
LOCUS       DASHHB010000045        21266 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.046, whole genome shotgun sequence.
ACCESSION   DASHHB010000045 DASHHB010000000
VERSION     DASHHB010000045.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21266)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21266)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21266
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.046"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(37..570)
                     /locus_tag="U2K71_001952"
     CDS             complement(37..570)
                     /locus_tag="U2K71_001952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HEM7316004.1"
                     /translation="MGVPKEGEFVTIQSYKHDGKLHRTWRDTMVLKTSEHSMIGVNDH
                     TLVTESDGRRWVTREPAIVYFHKKYWFNVIAMIREKGVSYYCNLASPFLLDDEALKYI
                     DYDLDIKVFPDGEKRLLDVDEYEMHSKMMNYPNDIDFILKENVKILVDWINNGDGPFS
                     EGYIDIWYNRYKQLSRK"
     gene            complement(664..1842)
                     /gene="mutY"
                     /locus_tag="U2K71_001953"
     CDS             complement(664..1842)
                     /gene="mutY"
                     /locus_tag="U2K71_001953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HEM7316005.1"
                     /translation="MNKEWEKWTDKETKEFQDQFIQWYEQEKRNLPWRYNRDPYRIWI
                     SEIMLQQTRVDTVIDYFYRFMEWFPTIEELANAPEEKLLKAWEGLGYYSRARNIQAAA
                     KQIMSEFDGEMPQTPEEISSLKGIGPYTTGAIASIAFGLPEPAVDGNVMRVVSRLFCI
                     EADIAKASSRKIFDEAMRKIIDEKHPGEFNQAMMDLGSAICTPTSPKCETCPIQAFCL
                     ANKRGIQTSFPVKTKKAKPKDVYYISAALQNHSGAYYFEERDSQKLLANMWTFPMMEV
                     TQEEYERLKKEWETKPEIDLFDDLVAEEEQNLPFEKQELFVWQTRHLGEVTHIFSHLK
                     WHVLLFYGRATEGAEQEFTENKTSKWLKPDAFDSVVFPKVQMKLVDQLEKNRNNRNPF
                     "
     gene            complement(1835..2632)
                     /gene="recX"
                     /locus_tag="U2K71_001954"
     CDS             complement(1835..2632)
                     /gene="recX"
                     /locus_tag="U2K71_001954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombination regulator RecX"
                     /protein_id="HEM7316006.1"
                     /translation="MLTVTRISKGKGPFYRVDLSEGEALQVSEDVLVRYRLLKGRELD
                     EETIQEIKKSSGADFGFHLAMNYLSYQLRSEKEIRTYLKEKEILQEDRNKIVARLKEL
                     DLVDDLVYGESYVRTNMRLSDKGPKKLAQQMQQKGLKPEIIEQALSQYTFEDQVQNAH
                     QTAEKAFGKNHGKSQKELLRKIQQTLMTKGFSQEVIQQAIADLPQEADQEIEYDYLVK
                     QGDKLWRRNLRFEPKKRNLKVKQSLYQKGFDLDMIQRFITEKEEESE"
     gene            2739..4121
                     /gene="rlmD"
                     /locus_tag="U2K71_001955"
     CDS             2739..4121
                     /gene="rlmD"
                     /locus_tag="U2K71_001955"
                     /EC_number="2.1.1.190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (uracil(1939)-C(5))-methyltransferase
                     RlmD"
                     /protein_id="HEM7316007.1"
                     /translation="MAETIVKTGQTVPLKIKRLGINGEGIGYFKRLIIFVPYALPKEE
                     VLVKITKATPRYAEGELIKVKKTSKDRVVAPCPVYYECGGCQLQHLAYQAQLDFKKDL
                     LLQALEKFKPAGFRSYQLLPTIGMDEPWHYRNKAQFQLRKNKQTQKIEAGLYEANSHQ
                     LVPITDCLVQEPRTQKVMNTVVQLLNKFDAPIYDERTNSGIFRTIMVRIGVKTGELQV
                     VFITRSKKFPQKNAMIREINERLPEVVSIMQNVQAQKTSLVMGDETIHLWGKEAIEEQ
                     INEVTFDLSPRAFFQLNPQQTELLYGEGIKALDVQPNETIVDAYCGVGTIGLSVAKQA
                     KEVRGMDTVPQAIADAKKNAERLGYHNTRYEVGTAEQVLPKWLNEGFQPDGIIVDPPR
                     TGLDNALIQSLLKYPPKKLVYISCNVSTLARDLVNLSKKFKIEYLQSVDMFPQTARCE
                     VVVKMTRLAD"
     gene            complement(4295..4873)
                     /locus_tag="U2K71_001956"
     CDS             complement(4295..4873)
                     /locus_tag="U2K71_001956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic polysaccharide monooxygenase"
                     /protein_id="HEM7316008.1"
                     /translation="MKKISTLLFIGLVSLGLFGFSIPGFAHGYITSPGSRAYLGTSAA
                     GNLNQNVGRAQWEPQSIEATKNTFIDGKIASAGVSGFEPLDEQTSNRWHKNMVNPGTL
                     NITWNLTAQHRTSTWDYYITKPTWNPNQPLKFSDFELITKIDDKATVPPKTVNQTITL
                     PQDRKGYNVILAVWNISDTTNAFYQVIDVNIQ"
     gene            5195..5665
                     /locus_tag="U2K71_001957"
     CDS             5195..5665
                     /locus_tag="U2K71_001957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan-rich sensory protein"
                     /protein_id="HEM7316009.1"
                     /translation="MKMIKDIRFWVCVIGIVILGFLSGLLSGNPGKYYYSLQLPPFAP
                     PSWIFGPMWTLLYILMGISLYLLLNHNNKKQRNNLVGLFVIQFIFNFIWSALFFNLRN
                     IFIAAIDITLLVIFLSVLMYQLWLHHRLAMWLMIPYYLWVLFATLLNYSIYFLN"
     gene            complement(5773..6999)
                     /locus_tag="U2K71_001958"
     CDS             complement(5773..6999)
                     /locus_tag="U2K71_001958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM7316010.1"
                     /translation="MKRWQKVLLTLLGVFVLLIAGLSAYGIRFMGEASQTVNKISKNS
                     NRISSKRSERVSIDDKEPFSVLLLGLDTGGLGRTEQGRSDTMMVVTVNPKQKKSTIIS
                     LDRDIYTNIVGYGTVDKLNHAYAFGGVEMAMDSIEQLLDIPIDHYVTINLDGMEDLIN
                     AVGGIKVNNKIDFTLDGVHVPVGEQTLNGEKGLAYSRMRHEDPEGDIGRQRRQREVVT
                     KIVNKVLSLDGVSNYRKILKAVENNVTTDLDWDDMLDVATNYTPAFETIKQDQLQGEG
                     QMINDIYYQILGKNDLLSIQNELKKQLNIKTSDTLPNLKNENASMMFFDDSEDGDSTN
                     GDGSNSEGYTDYSSYSENTYDQYQDPNTYYQQTEDTSQYYTPSQDTQSYDPYAGQEQN
                     GYGYDQNNEYGTTSGY"
     gene            complement(7753..7953)
                     /locus_tag="U2K71_001959"
     CDS             complement(7753..7953)
                     /locus_tag="U2K71_001959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003230776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM7316011.1"
                     /translation="MEHGTVKWFNNEKGFGFITVDGGDDVFVHFSAIQGDGFKSLEEG
                     QEVEFTIVEGARGPQAAEVTKL"
     gene            complement(8087..8695)
                     /locus_tag="U2K71_001960"
     CDS             complement(8087..8695)
                     /locus_tag="U2K71_001960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HEM7316012.1"
                     /translation="MTYTLPDLPYAYDALEPYIDEETMHLHHDKHHNTYVTNLNSAIE
                     KYPELGEKTIEELLSDMDAIPTDIKTAVRNNGGGHANHSFFWEIMAPNAGGEPTGEIK
                     EAINEAFGDFSSFKEEFKKAAAGRFGSGWAWLVMENGKLAITSTANQDSPLMEGKTPI
                     LGLDVWEHAYYLKYKNVRPDYIAAFWNVINWDEVNKRFEAAK"
     gene            complement(8780..9250)
                     /locus_tag="U2K71_001961"
     CDS             complement(8780..9250)
                     /locus_tag="U2K71_001961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HEM7316013.1"
                     /translation="MKQYRQLKGYLEAKGYWEILVYLFFGGLATIVNFVSFALARQYF
                     DLSMALSNSISWFCSVLFAFVTNKLWVFHSKSPNFTHALIECGKFFFYRILSYGLDMG
                     AMVLLINVMNSNEYVAKIITQIIVILANYIFSKLFIFKETEVFEEEVGQSEKDD"
     gene            complement(9298..10212)
                     /locus_tag="U2K71_001962"
     CDS             complement(9298..10212)
                     /locus_tag="U2K71_001962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEM7316014.1"
                     /translation="MEEGIVPTQPQRSSKKLFGVVVLNAVITLSEFIGGIMSGSLSLV
                     SDAFHNLSDTLAIIFSYGAQKMARKEANKKRTYGYQRLEILSAFINSFILIILSLFLT
                     VEAFKRFTSPEKINSHLMLTVAVIGLLANLFSVLLLRQEADESLNIKSSYLHLLSDTL
                     SSVSVIIGAILIRFFDIYWIDPVITLIISIYILIEAIMVIKKAAAILIQSAPAIDYEK
                     MEQEIEAIDGVKDVHHVHIWQYSEKIIIFDGHIDFEDQLLSEIEKIYPRIISLLKLKY
                     GITHVTIQAETKAKDQKKLIFLNKDESY"
     gene            10358..11161
                     /locus_tag="U2K71_001963"
     CDS             10358..11161
                     /locus_tag="U2K71_001963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1189 domain-containing protein"
                     /protein_id="HEM7316015.1"
                     /translation="MTTKQLIISSFIRFKDLKEARNVPFWKAIVYLLVLSIIMALPIS
                     FQVFQVLNNIQLDGQKIATRIPDFTIQNGQLQTEEKEGFIYQTNSIIFTFDPEGKRTE
                     QDISTDLMGNFVSVGMLKNKLIIALPNTGTTSALLNNNQLELPYTNESLKNLTGKQLR
                     SFLSEASIPLWIKVLTFIFSIYPSFLNLLITLLFTNIAAFLYARFRLTKATFLDCLKT
                     LIYSISLPTIIATILMVFLPSFDTSAFIAFAGIFIFAQAVKGWAKISIN"
     gene            11339..12286
                     /locus_tag="U2K71_001964"
     CDS             11339..12286
                     /locus_tag="U2K71_001964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HEM7316016.1"
                     /translation="MSERIIYADRPFRDEFIEEIHTIAPGYIFKTELQPEDLQNVEIS
                     LGWNSDYQEKLLASSTLKWVQSISAGVDQLPLETFSKKHILLSNGSGIHSESITEHVL
                     GIILGYSRGLFQAQKAQSDMKWLGSSVHYQSIQGKKILIIGTGHIGKMIAKKCKALDM
                     NCYGINTSGHPAEGMEKTFSLNQLKEVLPEVDFVVNILPLTEETTGLFDQHMFEHFDP
                     KSVFINVGRGASVKTQDLVQALNNQQLSFAALDVFEEEPLPQDHPLWKMDNVLITSHI
                     AGLTPDFQKKLMAIFLTNLKSYFATHELQTNQVKLSAGY"
     gene            complement(12354..12647)
                     /locus_tag="U2K71_001965"
     CDS             complement(12354..12647)
                     /locus_tag="U2K71_001965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis protein"
                     /protein_id="HEM7316017.1"
                     /translation="MKLTVTPKAQKWFDSEIDLPETYGIRFFGKVYGKTEVHDGFSIG
                     MSVEQPEHPVKKVEIDGTLFFIEEADEWFFKGYDLLVDYDPNLEEPTYHFKKQ"
     gene            complement(12675..13340)
                     /locus_tag="U2K71_001966"
     CDS             complement(12675..13340)
                     /locus_tag="U2K71_001966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TrkA family potassium uptake protein"
                     /protein_id="HEM7316018.1"
                     /translation="MKQNFAIVGLGRFGGSICRTLIEAGQEVLAIDSNEDRVNEYMNI
                     ATHAVVANAQDEMTLRSLGIRNFDHVIVAIGEDIQASILVTLMVKEMGVPNILAKAQN
                     EYHARVLEKIGADRVVHPERDMGIRIAHNLVSKNILDYLELSDKYSLAEIRVSNPKFF
                     NKTLAELNFRQRFDLTVVAIRRVDQTVIASPAADEYVRENDNLLVIGETGDVDILDDK
                     MNQ"
     gene            complement(13480..14112)
                     /locus_tag="U2K71_001967"
     CDS             complement(13480..14112)
                     /locus_tag="U2K71_001967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7316019.1"
                     /translation="MIKVLLVDDHEMVRLGVSFYLSIQEDIEVIGEAENGRQGYEKAM
                     TLRPDVILMDLVMEEMDGIESTKAILKDWPEAKIIIVTSFIDDEKVYPAIEAGAAGYL
                     LKTSTAHEIADAIRATQRGERVLEPEVTTKMMEKMSRRNEPVLHEELTNRENEILMLI
                     SEGKSNQEIADELFITLKTVKTHVSNILAKLEVEDRTQAAIYAFKHGLVK"
     gene            complement(14119..15186)
                     /locus_tag="U2K71_001968"
     CDS             complement(14119..15186)
                     /locus_tag="U2K71_001968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HEM7316020.1"
                     /translation="MMGKISRAMLAVYSGIAAFLIILFSLFTYFYASNQSHWWGELLR
                     ARLLYVPLIFHLIAISLGVGLIVFLLLSLIQKTKYGKIEEKLRALSSGNYESKLLLLP
                     IPSASDDLYIKDIDKEITKIKEKMIEVSSELQIVTSRPQYVDGQTKEEILELERHRLA
                     RELHDSVSQQLFAAMMMMSALTEQAEKSETPEMFRKQLKMVAEIINASQSEMRALLLH
                     LRPVNLEEKSLKQGIEQLLKELQNKIQISLKWDVEDVKLSSSIEDHLFRIVQELLSNT
                     LRHAKANELEVYLHKIDNNLLLRIIDDGTGFNMNETKTGSYGLNNIKERVAGIGGTVK
                     IISFKGQGTSVEIKVPLMKEA"
     gene            complement(15183..15914)
                     /locus_tag="U2K71_001969"
     CDS             complement(15183..15914)
                     /locus_tag="U2K71_001969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall-active antibiotics response protein"
                     /protein_id="HEM7316021.1"
                     /translation="MNSSWRFFVVVEALLLLFAVWQIVNNTGLLLLVIFGIFNIYLAM
                     RKHPRTKFQNFQLILGSLVIFFSLVNSPALWMMAVFAVLFIGLKGVEISGIDLTKNAF
                     WRKKQIMMIQTEQLKTHNNERKRQQLFGNQRIGNDVYEWDDINIAVISGDTIIDLGNT
                     LLPKDDNIVIVRKGIGRTRILVPLGVAIRLEHATLVGNVLFEEEQFSLKNEQIKIYSS
                     DYDENPRRLKIITNTLIGDVEVIRI"
     gene            complement(16083..16562)
                     /gene="greA"
                     /locus_tag="U2K71_001970"
     CDS             complement(16083..16562)
                     /gene="greA"
                     /locus_tag="U2K71_001970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016172390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HEM7316022.1"
                     /translation="MVEKVYPMTLEGKEKLEKELEELKTVKRGEIIERIKIARGFGDL
                     SENSEYESAKDEQAFVEGRITTIENMIRFAQIIDNDGVDADEVSIGKTVTFIELPDGE
                     EEEYTIVGSAEADPFSGKISNDSPIAQALIGKRKNDEVTIATPGGDMLVKIVKVEQA"
     gene            complement(16698..17873)
                     /gene="mltG"
                     /locus_tag="U2K71_001971"
     CDS             complement(16698..17873)
                     /gene="mltG"
                     /locus_tag="U2K71_001971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HEM7316023.1"
                     /translation="MSGNKQHPSKDKKEASKAATADIIDKRRNLRKKEDKIVRKIILV
                     IALTLLIIGGFLGFTVYRYVDSGLKPLDKSDDQLVQVEIPSGSSNKQIGEILEKDNII
                     KSGIVFNYYTKFKNLTGFQAGYYQLAPNMTLDEIGKQLQEGGTSEPTKVADGKIAIPE
                     GYDIDQIAERVAKVTGKDKKEFLDLVNDETFFNELHQKFPELLESASKAENTKYRLEG
                     YLFPATYDYYTSTSLKDLVIEMVNKTNTVMQNYYSAIKQKNLTVQEVLTLASLVEKEG
                     VKENDRKNIAQVFFNRIKANMPLQSDISVLYALGEHKELVTYEDTAVDSPYNLYTNTG
                     YGPGPFDNPSEEAIKAVLEPAENDYYYFVADTSTGNVYFAKTYEEHMELVEKYVNNS"
     gene            18077..19246
                     /locus_tag="U2K71_001972"
     CDS             18077..19246
                     /locus_tag="U2K71_001972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-lactamase family protein"
                     /protein_id="HEM7316024.1"
                     /translation="MKKGKHAKKRKYSFLMIVVVLFSLSAFSTGTILGWQFYNNEHSQ
                     IVAHAQSAQKKLILTTNEQQLVEKIDQLLRKNDYIGSIYVKKNNRIIMEKGYGYANKL
                     TQQPNDPSLYYQIGSVQKALTTLLILKQVERGHLSLDTKLAQFYPQIAGSQQITIRDL
                     LYMRSGLHRTASPTIPMTDEEVVQFAIQHLKFTDYQTSHYDSLNFTILTGILIKLTNQ
                     PYEKLLTDDIIEPLGLQHTKFYDQVKNSSNHALSYQMSKEDDYFKPLTESETEIHNEL
                     GTGNISMSVYDLNTLFTQVLSGKLVSKDLLFSLWRENANGHPYSGGVYSGDNYILSQG
                     NIDSFDTSAIFSKDSQDAIIMESNIKADKTIKLPTADLRNQIYDLMEGTTKLANS"
     gene            complement(19513..21147)
                     /locus_tag="U2K71_001973"
     CDS             complement(19513..21147)
                     /locus_tag="U2K71_001973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316025.1"
                     /translation="MRWYMNLKKIFSVKIFIVYAFVSLLYLFPAFKNMEIFGFYDTAF
                     HINRALSLESIFSSPINFETFRSYGMQVNNFYPWLTLYPLFLLIKFTNLAIGYNLFLY
                     IVTLITLFICHYVMYEITKKHVTSSFFAIIYTTSSFRSVEIFLRGAMGELLAMSILPL
                     ILLGFIKLYDSKKESWVMLAISMTLLIYTHVLSVAMTMVMIIIALIIQFYRKKKIEIF
                     LIIKLIKAAIVTLLLSLAFIGPMIEQTLYQDLNRPYVDILQKRAIYLGKEFLIGSIDS
                     ELLSFGIGLTLLICLIILTFNFKKLGILSKITYIMGIISILLCTKIFPWFALQNTPLN
                     IIQAPWRFMIFSTLFISFSVSLFISPQLEKFSYTRRRNFILLLILAISILNYSSVVNM
                     VKEMNFQGTYSNEQMENKLEEFENFDYVPIGYKKDQLLLKNHQVTEDGQVIYSSFSPN
                     KDEITFTFKSSKGKTIVLPVFRYKGLTVTVNSKNATVLKQGGPFVTVKSESGINKVTI
                     RYSYTFFSKISLFISIITLIVLLFYCIKLKIRTSHE"
BASE COUNT         6554 a         4154 c         3341 g         7217 t
ORIGIN      
        1 aaaaatctct cactggctta ctgttctttt ttgtgtttac ttacgcgaca attgcttgta
       61 acggttatac caaatatcaa tatagccttc cgagaacggg ccatctccat tattgatcca
      121 gtcaaccaga atcttcacat tttcttttag gatgaagtca atatcgttcg ggtaattcat
      181 cattttgctg tgcatctcat attcatcgac atccaatagt cgtttctcgc cgtcaggaaa
      241 taccttgata tccaagtcat agtcgatata tttcaaggcc tcatcgtcca gcaaaaaagg
      301 tgaagctaga ttacaataat aggaaacccc cttttctctg atcattgcta ttacattgaa
      361 ccagtatttt ttatgaaaat aaacaatcgc tggttcgcgc gttacccatc ggcgtccatc
      421 agattcagtg actaatgtgt gatcattcac gccgatcata gagtgttcgc ttgtttttaa
      481 taccatggta tctcgccacg ttcgatgcaa tttgccgtcg tgcttgtaac tttggatcgt
      541 cacaaactct ccttcttttg gaactcccat agcaaccctt ccttaagcag cattttgtgc
      601 tttgtgtttg ggtgttcatt acaccgacat cataattatt atatcaataa aaatgagctt
      661 tgcctagaaa ggattcctat tgtttcgatt tttctctaac tgatctacca gtttcatttg
      721 gaccttagga aaaacgaccg aatcaaatgc atcaggcttc aaccacttcg aagttttgtt
      781 ctcggtaaat tcttgttctg ctccctcggt tgcccgtccg taaaaaagga gaacatgcca
      841 tttcagatga ctaaaaatat gagtaacttc tcctaaatgt cgtgtctgcc aaacaaagag
      901 ttcctgcttt tcaaatggga gattttgctc ttcttctgct actaggtcat caaacagatc
      961 gatttctggc tttgtttccc attctttttt caatcgctca tactcctctt gtgtcacttc
     1021 catcattgga aatgtccaca tattcgccag caatttttgg ctatctcttt cttcaaaata
     1081 atacgctcca gaatgatttt gaagagctgc actgatataa taaacgtctt ttggctttgc
     1141 tttttttgtt tttacaggaa aactcgtttg gattcctcgt ttgttagcta aacaaaaggc
     1201 ttggatcgga caagtctcac actttggaga agttggtgta cagatagctg aacctagatc
     1261 catcattgct tggttgaatt cacctggatg cttttcatca atgattttcc gcatcgcttc
     1321 atcaaagatt ttcctgctag aagcttttgc gatgtctgct tcaatgcaaa acaatctgct
     1381 gactactcgc atcacgttgc cgtcaactgc aggttctgga agtccaaatg cgatacttgc
     1441 aatcgcgcct gtcgtatatg gtcctattcc cttcaatgag ctaatttctt caggcgtttg
     1501 cggcatttcc ccatcaaatt ctgacatgat ctgtttagca gcagcttgga tgttccgggc
     1561 tcttgagtaa taaccaaggc cctcccatgc ttttaaaagc ttttcttctg gagcatttgc
     1621 tagctcttca attgtaggaa accattccat gaaacgataa aaataatcga tcactgtgtc
     1681 tactctggtt tgctgaagca tgatctcaga gatccagata cgataaggat cacgattata
     1741 gcgccaaggc aggtttcttt tttcttgttc ataccattga atgaactgat cttgaaattc
     1801 ttttgtttct ttatctgtcc atttttccca ttctttattc actttcctct tccttttccg
     1861 taatgaatcg ctggatcata tccaaatcaa atcctttttg atataagctt tgctttactt
     1921 ttagatttct ttttttaggt tcaaacctta aatttcttcg ccatagttta tctccttgtt
     1981 tgactaaata atcatattct atttcttgat ctgcttcttg cggaagatca gctatcgctt
     2041 gttggatcac ttcttgagaa aagcctttcg tcatcaatgt ttgttggatt tttctcaata
     2101 attctttttg gctttttccg tgattttttc caaacgcttt ttcggctgtc tgatgtgcat
     2161 tttgaacttg gtcttcaaat gtatattggc tgagcgcttg ttcaatgatt tcaggtttca
     2221 aacctttttg ctgcatctgc tgcgctaatt tctttggtcc tttgtcactt aagcgcatat
     2281 tcgtgcgcac ataactttcc ccatagacca aatcatcgac aaggtccaat tcttttaagc
     2341 gggctacgat tttatttcga tcttcttgaa ggatttcctt ttcttttaag tatgttcgga
     2401 tttctttttc cgatcgcaac tgataactta agtaattcat tgctaggtga aaaccgaaat
     2461 cagctccgct actcttcttg atctcttgga tagtttcttc gtctaattcc cgtcctttta
     2521 ataatcgata gcgtactaat acatcttcag atacttgtaa agcttctcct tcagataaat
     2581 ctacgcgata gaaagggcct ttgcctttac tgattctcgt aacggttaac ataaacatcc
     2641 tcctcgaaca tatattctct atcataccac agaaaatccc cgtaatcagg tattatatgt
     2701 tagaataaac cacagaaaaa agaaaaaggg agatacttat ggcagaaaca attgtcaaaa
     2761 ctggacaaac ggttcctttg aaaatcaagc gtttaggcat aaatggtgaa ggaatcggtt
     2821 atttcaaacg gttgatcata tttgttccat atgcactacc aaaagaagaa gtactagtga
     2881 aaatcacgaa agcaactcct cgctatgcag aaggagaatt aataaaagtc aaaaaaacaa
     2941 gcaaagatcg cgtagtggca ccatgtcctg tttactatga atgcggtggc tgtcaattac
     3001 aacacttagc ttatcaagct caattggatt ttaaaaagga tctgcttttg caagcattgg
     3061 aaaaattcaa accagcaggc tttcgatctt atcaactttt gccgacgatc gggatggatg
     3121 agccttggca ttatagaaac aaagcacaat ttcagttacg aaaaaataag cagactcaaa
     3181 aaattgaagc tggtttatac gaagcaaact ctcatcagtt agtccctata acagactgct
     3241 tggttcaaga acctcgaaca caaaaagtaa tgaatactgt tgttcaacta ttgaataaat
     3301 ttgatgcacc catctatgat gaacggacaa acagtggaat cttccgcaca atcatggtta
     3361 gaatcggtgt taaaactggg gagttacaag ttgtttttat tactcgcagt aaaaaattcc
     3421 cgcaaaaaaa cgcaatgatc cgtgagatca atgaacgact gcccgaagtt gtttcaatca
     3481 tgcaaaatgt ccaggcacaa aaaacgtctc tcgtgatggg ggatgaaacg atccacttat
     3541 ggggaaaaga agccattgaa gaacaaatca atgaagtgac tttcgatctt tctcctcgtg
     3601 ccttctttca actcaatcct cagcagaccg aattgcttta tggcgaagga atcaaagcat
     3661 tagatgtcca gcctaatgaa acgatcgtgg atgcttattg cggagttggg acgatcggct
     3721 taagtgtagc aaaacaagcc aaagaagtac ggggaatgga taccgttcct caagcaatcg
     3781 ctgatgcaaa aaaaaatgcg gaaagattag gatatcacaa cactcgatat gaagtcggga
     3841 cagcagagca agttctgcct aaatggttga atgaaggttt tcagcctgat ggtatcatcg
     3901 tagatccacc acgtacgggt ttggataacg cattgatcca atcattattg aaataccctc
     3961 caaaaaaact tgtttatatc tcctgtaatg tttctacact tgccagagat ctggttaatt
     4021 taagtaaaaa gttcaagatt gaatatttac agtccgtcga catgtttcct cagactgcac
     4081 gttgtgaagt tgtcgtgaag atgacgagac tcgctgacta aatgaaagag gttgtgaaaa
     4141 agtctgccct gtatcaagta ataagaacag ccaaacaaaa atagctcttc atgttttatg
     4201 aatactgtac ttgtgaccga agagttaaca ggaggacact atttatttaa aaaaagggtt
     4261 gtgacatagt ttttgtcaca gccctttttg attattattg gatattcacg tctatcactt
     4321 gataaaatgc atttgtcgta tcgctgatat tccatactgc taaaatcaca ttatatcctt
     4381 ttcgatcctg cggaagtgtg atcgtttggt tgactgtttt tggtggaaca gttgctttgt
     4441 cgtctatttt tgtgatcaat tcaaaatcac taaatttcaa aggttgatta ggattccaag
     4501 ttggtttggt aatgtagtaa tcccaagtac ttgtccgatg ttgtgctgtt aaattccaag
     4561 taatatttaa tgttcctgga ttaaccatgt ttttatgcca tctgttagat gtctgttcat
     4621 ctaaaggctc aaagccgctg actcctgcac tagcaatttt tccatcaatg aatgtatttt
     4681 tggtagcttc gatactttgc ggttcccatt gtgctcttcc gacattttga tttaagtttc
     4741 ctgctgctga tgttcctagg tatgcacggc ttcctggaga agtaatataa ccatgcgcaa
     4801 aacctggaat gctaaaaccg aacaacccta aactaactaa cccaataaac aataacgtac
     4861 taatcttttt catgtagaaa cctccttcat ttgataacca cattgtaaac gctttattgt
     4921 attaacacaa acattatgct tttataattt tatgttgaag taaaaaaggt gaaaaaagta
     4981 agatgcagat tataacagaa gtagacgaga aaagtacaaa tagaaaacat gagaatcaaa
     5041 tcatatgatc tttttcaaaa acatcattca tataaacaaa tgttcaactt tttaataaca
     5101 acaattatca atttttttct tacttcacaa actaaataca ggtcttgctg tgagccttca
     5161 tggtaaacta atattataga aaggagttag taaaatgaag atgataaaag atattcgttt
     5221 ttgggtttgt gttataggaa ttgtcatatt aggcttttta tcagggttgc ttagcggaaa
     5281 tcccggaaaa tattactatt ctttgcaact tccaccattt gcaccaccta gctggatatt
     5341 tgggccgatg tggaccttac tgtatatatt gatgggtatt tctctttatt tgttacttaa
     5401 tcacaacaac aaaaaacagc gaaacaatct tgtcggtctt tttgtgattc agttcatttt
     5461 taattttata tggtctgctc tattttttaa tttaagaaat attttcattg cagcaatcga
     5521 tattacactt ttagttattt tcttgtcagt gcttatgtat cagttgtggc tgcatcatcg
     5581 cctggcaatg tggctcatga ttccttatta tttatgggta ctattcgcaa ctctattgaa
     5641 ttattcaatt tattttttaa actaagcgct aaaaagagac gtcctgtaat ttaacacata
     5701 gaaaaagtgc cacctaagtt agattttttg tctaatttag gtggcactct ttattaacca
     5761 aactattttt ttctagtacc cacttgttgt tccatactca ttattctggt cataaccata
     5821 accattttgt tcctgtccag cgtaaggatc ataactttga gtatcttgac ttggtgtata
     5881 gtactgactt gtatcttctg tttgctgata ataggtattc ggatcttggt actgatcata
     5941 tgtgttttct gaataggaag aataatcggt gtacccttca ctattcgatc catcaccgtt
     6001 tgtcgaatcg ccatcttccg aatcatcaaa gaacatcatt gaagcattct catttttcaa
     6061 attaggtaat gtatcgcttg tttttatatt taattgtttc ttcagttcat tttggatcga
     6121 aagaagatca ttttttccta aaatttgata gtagatatca ttgatcattt gtccttcgcc
     6181 ttgcaactgg tcttgtttga ttgtttcaaa cgccggggtg tagttcgtcg ctacatcaag
     6241 catgtcatcc caatctaagt ccgtcgtcac attgttctca actgctttta aaatttttct
     6301 ataattgctt acgccatcta aacttaaaac tttattgacg attttcgtca ctacttcccg
     6361 ctgacgacgt tgtcgaccga tatcgccttc tggatcttcg tgtcgcattc gagaataggc
     6421 taaacctttt tctccattca atgtttgttc acctactggt acatggacac catccaaagt
     6481 aaagtcaatt ttattattca ctttgatgcc accgaccgca ttgatcaaat cttccatgcc
     6541 atccaaattg atcgttacat aatgatcaat aggaatatcg agcagttgct caattgaatc
     6601 catggccatt tcgacaccac caaatgcgta ggcatgattt aatttatcta ccgttccata
     6661 acctacgata ttcgtataaa tatctcggtc caaactgatg atcgttgatt ttttttgttt
     6721 cggattaact gttactacca tcatcgtatc agaacgtcct tgttccgttc ttcctaatcc
     6781 accggtatct aaacctaata gcaaaaccga aaaaggttct ttgtcatcaa tgcttactct
     6841 ctcagaacgt ttggaggata ttcgattaga gtttttagaa attttgttta ctgtttggct
     6901 cgcttctccc ataaatctga ttccgtatgc tgataatccg gcaatcagca aaacgaatac
     6961 ccctaacaag gttaatagaa ctttttgcca tcgcttcata atacacctct gtttcacatt
     7021 ttctaaaata ttgtctcata taactgttca atcagcaaat tcacttttta atcataagaa
     7081 aactttaaaa aacatttttg tgtaacgatt attatttttt aaaatttctc aaaattctta
     7141 actatcatac cataccgtga aaaagatcga aagaccgaat acacgcagtt cgcaatcgtt
     7201 ttatcttttt ttatgtattt tttccagaaa aagcgaacaa caaggtattt tccaaataat
     7261 cgtgcagtaa aatgatcgca catacctttc ccatcttttt aacagtcttt gcctaccgtt
     7321 cagattatca ttcgatttca cgacaatttc ttcctatgac tggtcctttt gttaaaccca
     7381 tcttctattc gttttatttc cagctcactg catggctctt aaaatgactc gaactagtga
     7441 ttgcattacc atcatctttt aatggataga tatgctcctc ttcaaagtaa aacgattttc
     7501 ttttaaagta atagaattga catggtaatg agtatgtata ctagtggttt cacaaatggt
     7561 cttttattca acatccaaaa actgaaattt ctttaaaatc cattactaac gaagaaaatg
     7621 ttccttgctt gataattagt taggacatgt caagtgaaca aatgcattta cttagccaaa
     7681 aaatctaaaa acttcataaa tagacaaaaa gacacgactc tctaagaagt cgtgtcttta
     7741 ttcaacaaat tattataatt tagtcacttc agcagcttga ggtccgcgtg caccttcaac
     7801 aatcgtgaat tctacttctt gaccttcttc taggctcttg aagccgtctc cttgaattgc
     7861 tgagaaatga acaaatacgt catctccgcc gtctactgta ataaacccaa atcctttttc
     7921 attgttgaac catttaaccg ttccgtgttc cataaatgta tatcctccaa aaaagttctt
     7981 gcaagtaacc ttgcaagaac attataccca atttagaaaa cgctagcaag ctttcgctct
     8041 tttattttcc aaattatttt taagactgat atgacggagg aaatggttat tttgctgctt
     8101 cgaaacgttt gtttacttca tcccaattaa taacattcca gaatgctgca atataatctg
     8161 gtcggacatt tttatatttc aaataataag catgttccca aacgtccaaa cctagaattg
     8221 gtgtctttcc ttccatcaat ggagaatctt gatttgcagt agaggtaata gctaattttc
     8281 cattttccat tacaagccaa gcccatccag aaccaaatcg tccagcggct gcttttttga
     8341 attcttcttt aaaagaagaa aaatcaccaa aagcttcatt aatcgcttct tttatttctc
     8401 ctgtaggttc gccaccagca tttggtgcca tgatttccca gaaaaatgaa tggttagcat
     8461 gtccgccacc attgttacgt acagctgtct tgatatctgt tggaatagcg tccatatcag
     8521 ataataattc ttctattgtt ttttcgccta attctgggta tttctcaatt gctgaattta
     8581 aattcgtcac ataagtattg tgatgcttat catgatgcag atgcatcgtt tcttcgtcaa
     8641 tataaggttc caatgcgtca tatgcataag gtagatctgg taatgtgtaa gtcataaaaa
     8701 aattcctcct caatgttttc ttaataatgt ctacattaca aagagtaaca aagagaagga
     8761 aattcttcaa ttgttatgcc tagtcgtctt tttcactttg acctacttct tcttcaaaaa
     8821 cctcagtttc tttaaaaata aacaacttgc tgaaaatata gttcgcaagg ataacaataa
     8881 tttgagtgat gattttcgcc acatactcat tgctgttcat aacattgatc aacaatacca
     8941 ttgcccccat gtccaatccg taagaaagga tacggtagaa gaagaactta ccgcattcaa
     9001 taagtgcatg tgtaaaattc ggtgattttg aatggaatac ccaaagtttg ttcgttacaa
     9061 aagcaaacaa tacagaacaa aaccacgaaa tagaattgga taaggccata cttaaatcaa
     9121 aatattggcg tgcgagagca aaactaacaa aatttacaat cgtagccaaa ccaccaaaaa
     9181 agagatagac tagtatctcc caatatcctt ttgcttctaa atatcctttt aactgtcgat
     9241 actgcttcat gagccaacct accttattaa aaattcttat ttatcatact ctatatttca
     9301 ataactttca tctttattta aaaaaattaa ttttttttga tcctttgcct ttgtctctgc
     9361 ttggatcgtc acatgtgtaa taccatattt taattttaat aaagaaatta tacgaggata
     9421 tatcttttca atctcgctta gtagctgatc ttcaaaatca atatgtccgt cgaaaataat
     9481 tatcttttcg ctatactgcc aaatatggac atgatgaaca tcctttaccc catcgattgc
     9541 ttcaatttct tgttccattt tttcatagtc gatagcaggt gcactttgta tcaaaatagc
     9601 ggcagctttt ttgatcacca ttatcgcttc tatcaatata tatatcgaaa tgatcagtgt
     9661 aataactgga tcgatccagt agatatcgaa aaaacggatc aaaattgctc cgataatcac
     9721 acttaccgaa gacagtgtat cacttaacaa atgcaaataa ctagatttta tattcaaact
     9781 ttcatctgct tcttgtcgta ataataatac tgagaacaaa ttcgcgagca atccaataac
     9841 tgctactgtt agcatcaaat gactatttat cttttctggt gaagtgaacc gcttgaatgc
     9901 ttcaacggtt aaaaacaagg ataatatgat caatatgaat gagttgatga aagcactcaa
     9961 tatttctaaa cgctggtagc catacgtacg ttttttattt gcttcttttc gggccatttt
    10021 ttgtgccccg taagaaaaaa taattgccaa tgtatcacta aggttatgga aagcatctga
    10081 aacaagagac aaactccctg acataatccc tccaataaat tctgataaag tgatcaccgc
    10141 attcaaaacc actactccaa atagtttttt tgatgatctc tgtggctgtg taggcactat
    10201 tccttcctcc attttattta tattggatag tctttagaca agaataacat ttttttgata
    10261 tttcaccaat cattcagctg tttccttaaa gcacttgtat ttcacttgat acttcgctag
    10321 aattgcaagt gatgacacta ctagatgagg agtacttatg acaacaaaac aattaattat
    10381 cagttccttt atacgattca aagacctaaa ggaggcgaga aatgtcccat tttggaaagc
    10441 cattgtctat cttttagttt tgagtataat tatggctttg ccaatctctt ttcaagtttt
    10501 tcaagtctta aacaacattc aattggatgg gcaaaagatt gctactcgca ttcctgattt
    10561 tacaatccaa aacgggcagc ttcaaaccga agaaaaagaa ggattcattt accaaacaaa
    10621 ctccattatc ttcacatttg atccagaggg aaaacgaaca gaacaggaca tctctactga
    10681 tttgatgggg aattttgtta gtgtcgggat gttaaaaaac aaacttatca tagctcttcc
    10741 taatacaggc acgacttctg ctttgttgaa caataaccaa ttagaacttc cctacaccaa
    10801 tgaatcttta aaaaacttaa caggtaagca gctccgttct tttttaagtg aagcatctat
    10861 accactttgg ataaaagtcc tgacatttat attttctatt tatcctagtt ttctgaatct
    10921 gctgatcact ttgctattta ccaacattgc tgcttttctc tatgcacgct ttcgactaac
    10981 aaaagctact tttcttgatt gtttaaaaac attgatctat tcaatttcat tgcctactat
    11041 aattgcaaca atcttgatgg tctttcttcc ttcttttgac actagcgcct tcattgcttt
    11101 tgcaggaatc tttatttttg ctcaagctgt caaaggatgg gcaaaaatat cgatcaatta
    11161 agaaataaag acaactcgtt atttctactt caatagaccc ttttagaggt tgtgacattt
    11221 ttttgtcaca gccccttttt tatttgaaaa atatttcttt ctcaaaaaac aagcaaggcg
    11281 cttcaaagtt cttcttgtat gcgctacact tagagtaaag aaaggcggga aattaactat
    11341 gtcagaaaga atcatttatg cagatcgtcc atttcgtgat gaatttatcg aagaaatcca
    11401 tactatagcg cctggttata tctttaaaac agaattacaa ccagaggatc tacaaaatgt
    11461 agagatcagt ttgggctgga actcagacta ccaagaaaaa ctattagcct cttctacctt
    11521 gaaatgggtc caatctatct cagctggtgt tgatcagcta ccccttgaga ctttttcaaa
    11581 gaaacatatt ttactttcaa atggtagtgg catccacagt gaatcgatta cggagcatgt
    11641 cttaggaatc atccttgggt attctcgtgg tctttttcaa gcacaaaagg cacagagtga
    11701 catgaaatgg ttaggttcat cggtccatta ccaatctatc caagggaaaa aaatactaat
    11761 cattggaact ggccatattg gaaaaatgat tgcaaaaaaa tgcaaagcat tagacatgaa
    11821 ctgctatgga atcaatacat ccggtcatcc agcagaagga atggaaaaga ctttttcttt
    11881 aaatcaacta aaggaagttc ttcctgaagt agatttcgta gtcaatatct tacctctaac
    11941 ggaggaaaca actgggctct ttgatcagca tatgtttgaa cactttgatc caaaatctgt
    12001 attcatcaat gttggccgtg gtgcttctgt taagacacaa gatctggtgc aggctttaaa
    12061 taaccagcaa ctttcctttg cagcattaga cgtttttgag gaagagcctc ttccacaaga
    12121 tcatcctctt tggaaaatgg acaatgtact gattacttcc cacattgcag gtttgacacc
    12181 tgattttcaa aagaaattaa tggctatttt tttaaccaat ctgaaaagct actttgccac
    12241 ccatgagctt caaaccaatc aagtaaaatt gtctgcagga tattaatagc tgaaaacaat
    12301 attttcaaaa gaaaaaagag aaggtgacaa aaattgtgtc accctctctt tttttattgt
    12361 tttttaaagt gataggttgg ctcctccagg ttgggatcat aatcaacaag taaatcatat
    12421 cctttaaaga accattcatc tgcttcctcg ataaaaaaaa gtgtcccatc aatttccact
    12481 ttctttacag gatgttctgg ctgttcaact gacattccaa tcgaaaaacc atcatgaacc
    12541 tctgttttgc cgtagacttt tccaaaaaaa cgtattccgt aagtttctgg cagatcaatt
    12601 tctgagtcaa accacttttg tgcttttggc gttaccgtta atttcatccc aacaacctac
    12661 tttctctttt ctttctattg attcatctta tcgtctagaa tatccacatc gcctgtctct
    12721 ccaataacca gtagattatc attttcacga acatattcat ctgctgcagg agaagcgata
    12781 accgtttgat ctactcttcg tatggcgact actgttaagt caaaacgttg cctgaaattt
    12841 aattcagcta atgttttgtt gaaaaattta ggattggaga ctcggatttc tgctaaagaa
    12901 tatttatctg acaactctag gtaatctaaa atattctttg acactaaatt atgcgcaatc
    12961 cttattccca tgtctcgctc gggatggacg acgcgatcag ctccgatctt ttcaagtact
    13021 ctggcatgat actcattttg ggcttttgct aaaatatttg gtacgcccat ttcctttacc
    13081 atcagagtaa ctaagatact ggcttgaata tcttctccaa tagcgacaat tacatgatcg
    13141 aaatttctta tgcctaatga tcgaagtgtc atttcatcct gtgcatttgc cacaacagca
    13201 tgagtggcta tattcatata ttcattgacc cgatcttcat tactatcaat agccagtact
    13261 tcttgaccag cttcgatcag tgttcggcag atacttccgc caaatcgtcc aagacctaca
    13321 attgcaaaat tttgtttcac cgttttcctc ctttagtatc ttcttttctc agtataacaa
    13381 agcctttaca aaaacaaaag caagcatcat tattgtaaaa aagccaagaa aaataatagt
    13441 tattttctca gcttttgatt atcctcttta tcatcgtttt tatttcacca aaccatgttt
    13501 gaatgcatag atagcggctt gtgtacggtc ttccacttct agcttagcca atatattgga
    13561 aacatgtgtt tttactgttt tcagtgtaat aaacaattcg tccgctattt cctgattact
    13621 ttttccttca gaaatcagca ttaaaatttc attttcccga ttcgttagtt cttcatgtaa
    13681 tacaggctca tttcgccgac tcattttttc catcattttt gttgtcactt ctggttctaa
    13741 tactcgctcg ccacgttgcg ttgcccttat agcatcagca atttcatgtg cggtagaggt
    13801 cttcaacaaa taccctgctg cacctgcttc aatggcagga taaacttttt cgtcatcaat
    13861 aaaactggtt acgataatga ttttagcttc tggccaatct tttaagattg cttttgttga
    13921 ttcgatacca tccatttctt ccatgactaa gtccattaaa atgacgtccg gtcgaagtgt
    13981 catcgccttc tcatagcctt gtcgtccatt ttcagcttct ccaattactt caatgtcttc
    14041 ttgaatagat aaataaaaag agacgcctaa gcgcaccatt tcatggtcat ctactaataa
    14101 aacttttatc atccttatct atgcctcctt catcaaaggg accttgattt ctacacttgt
    14161 accttgacct ttaaaactaa tgatttttac tgttccacca ataccagcta ctcgttcttt
    14221 gatattgttc aatccataac ttcccgtttt cgtttcattc atattaaacc ctgttccgtc
    14281 gtcaataata cgtaacaaaa gattattgtc tattttgtgc aaatatacct ctaattcatt
    14341 ggctttagca tgtcttaatg tatttgataa caattcttga acgattcgga acaagtgatc
    14401 ctcaatggaa ctagatagtt ttacatcttc tacatcccat ttcagcgaaa tctggatttt
    14461 attttgcaat tccttcaata actgctcgat accttgcttt aaacttttct cttctaaatt
    14521 gactggacgt agatgaagaa gcagcgcacg catctcagac tgggaagcgt tgatgatctc
    14581 tgctaccatt ttcaactgct tgcgaaacat ttctggtgtc tcgctttttt ctgcttgctc
    14641 cgtcaatgcc gacatcatca tcattgctgc aaacaattgt tgcgaaactg aatcatgcag
    14701 ctcacgggct aatcgatgtc tttctaattc taaaatttct tctttggttt gtccatctac
    14761 atattgggga cggctcgtta caatttgtaa ttcactagat acttcaatca ttttttcttt
    14821 tatcttagtg atttccttat caatatcttt gatgtataaa tcgtccgatg cacttgggat
    14881 cggaagaagc aacaatttgg attcataatt gccagaagaa agtgcacgca gcttttcttc
    14941 gattttcccg tattttgtct tttgaataag tgataataat agaaagacaa ttaatcctac
    15001 acctaaggaa atggctatca aatggaagat aagcggaaca tataataaac gtgcacgcag
    15061 taattctccc caccaatgac tttgattgct ggcataaaaa taagtgaaaa gtgaaaatag
    15121 aataataagg aaagccgcaa tccccgaata aacagctagc atcgctctgg atatttttcc
    15181 catcatattc gtatcacctc gacatctcca atcaaggtat tagtaataat cttcaatcta
    15241 cgagggtttt catcataatc actgctgtag attttgattt gttcattttt caaagaaaat
    15301 tgctcttctt caaacaagac atttcctact aatgtcgcat gctctaatcg gatggctacg
    15361 cccaaaggta ctaagatccg tgttctgcct atcccttttc ttacaatcac aatattatca
    15421 tcttttggca aaagcgtatt gcccaaatct atgatagtat caccagaaat gacggcgatg
    15481 ttgatatcgt cccattcata gacgtcgttt ccaatacgtt ggttaccgaa taattgctgt
    15541 cttttccgct cattattatg tgtttttaat tgctctgttt ggatcatcat gatctgtttc
    15601 tttcgccaaa aagcattttt ggttaaatct atccctgaaa tctctacacc tttcagacca
    15661 ataaataaaa cggcaaatac agccatcatc cataaagcag gactgttgac taagctaaaa
    15721 aaaatgacca aacttcctaa tatcaattga aaattttgaa atttagtacg aggatgtttg
    15781 cgcatagcta gatatatatt gaaaataccg aatatcacta aaagcaaaag tccagtattg
    15841 tttacgattt gccaaacagc aaataacagc agtagtgctt caactaccac aaaaaaacgc
    15901 caagaactat tcatcgctct ttctccctcc tgacacattc tcatttcatc atacctattt
    15961 ttttaactgt tggctatagg actttagatg taagatcgtt tgttgaaatc taaaatacac
    16021 aaagagaaaa agatctagag tgtatcatca gtaagatgaa ctctctagat ctttcagtca
    16081 gactacgctt gttctacttt gacgatttta actaacatat cgccacctgg agtagcaata
    16141 gttacttcat catttttacg tttgccaatc aatgcttgcg caataggaga atcattggaa
    16201 attttgccag aaaacggatc tgcttcagcg cttccgacaa tcgtgtactc ttcttcttct
    16261 ccatctggta gttcgatgaa tgtaactgtt ttaccaattg atacctcatc tgcatctaca
    16321 ccgtcgttat cgatgatttg agcaaaacga atcatatttt caattgttgt gattcttcct
    16381 tcaacaaatg cttgttcatc tttcgctgat tcatattcag agttttcgga aagatctccg
    16441 aaaccgcggg caattttgat tcgttcaatg atttctccgc gtttgactgt ttttagttct
    16501 tctaattctt tttctaattt ttctttacct tctagagtca ttggataaac tttctctacc
    16561 atgtgtaacg tctccttttt tcgataaaat accgcaaaag gtaatctttt gaggaatata
    16621 gtccaaaaaa gattaccacg cttttcttca aatgtaaatg tttttctaaa aatgatcaat
    16681 gtctagagcc tgtttttcta agaattattt acatattttt ccactaattc catatgttct
    16741 tcataagttt tagcgaaata aacatttcca gtgctcgtat ctgctacgaa ataataatag
    16801 tcattttctg ctggttctaa tactgccttg attgcctctt cgcttggatt gtcaaaaggt
    16861 ccaggtccat agccggtatt ggtatacaaa ttatatggtg aatctacagc tgtatcttca
    16921 tacgtcacta attctttgtg ttcacctaat gcgtataaaa cagagatatc tgactgaagt
    16981 ggcatatttg ctttgattcg gttaaagaag acttgtgcaa tgtttttacg atcattttct
    17041 tttacgcctt ctttttcaac tagagaagct agagttaaaa cttcttgtac agtcaaattt
    17101 ttctgcttga tagctgaata atagttttgc atgaccgtat tcgtcttgtt caccatttca
    17161 atcactagat ccttaagcga agtactcgtg taataatcat atgtggctgg gaataagtaa
    17221 ccttccaaac ggtattttgt gttctcagct ttagatgcac tttccaacag ttctgggaat
    17281 ttttggtgca actcgttaaa gaaagtctca tcattcacta aatctaaaaa ttctttcttg
    17341 tctttaccag ttaccttcgc tacccgttca gcaatctgat cgatatcgta cccttctgga
    17401 atggcaattt tcccatctgc aacttttgtt ggctcagaag taccgccttc ttgcaactgt
    17461 ttgccaatct cgtcaagagt catattaggc gctaactgat aatagccagc ttgaaagcca
    17521 gttaaatttt taaattttgt ataataattg aaaacaatcc cgcttttaat aatattgtcc
    17581 ttctccaaaa tctcaccaat ctgcttgtta gaagagccac tcgggatctc gacttggact
    17641 aattggtcat ctgatttatc taaaggcttc aacccgctat ctacataacg atacacggta
    17701 aatccaagaa atccgccaat aatcaataat gtaagagcaa tcactaaaat gatcttcctc
    17761 acaatcttat cttctttttt acgcagattt ctccgcttgt cgattatatc tgctgtagct
    17821 gccttactgg cttctttttt atctttactt gggtgttgtt tgtttcctga caaaacaact
    17881 cctccttact atcaagttcg taactttatt cattatacat gatatttgaa ttggcgtgaa
    17941 tgattttatt taaatattct atcaaaaatg taagtagtaa atccattact ataagattaa
    18001 aataaatgtt atcaagatac aagctatttt agctttattc tttctgtaaa aaagaaagga
    18061 gactaaataa actattatga aaaaaggaaa acatgctaaa aaaagaaaat attcattttt
    18121 aatgatcgtt gtcgtgttgt ttagccttag tgcgttctct acaggaacta ttcttggatg
    18181 gcaattttac aataatgaac actctcagat agttgcccat gctcaatcag ctcaaaaaaa
    18241 gttgatactg actaccaatg aacaacagct cgttgaaaaa atcgatcaac tattaagaaa
    18301 aaatgattat attggctcaa tttacgtaaa gaaaaataat cgcatcatca tggaaaaagg
    18361 ctatggctat gcgaataaac tcacgcaaca accaaatgat ccttctcttt attatcagat
    18421 tggttcggtt caaaaagcat tgactactct tttgatttta aaacaagtcg agcgtgggca
    18481 cctttcttta gatactaagc tggctcaatt ctatccacaa attgctggca gccagcaaat
    18541 tactatccgt gacttgctgt atatgcgctc aggcttgcac cgaactgctt ctccaaccat
    18601 accgatgact gatgaagaag tcgttcaatt tgcgattcag cacttaaaat ttacagatta
    18661 tcagacctct cattatgatt cattgaactt taccatattg actggaattt tgatcaaatt
    18721 aacaaatcaa ccgtatgaaa aattattgac cgatgatatc atcgaacctt taggattaca
    18781 gcatacaaaa ttttatgacc aagtcaaaaa ttcatccaat catgcactat cctatcaaat
    18841 gtctaaagag gatgattact ttaaaccact gacagaatct gaaacagaga tccataacga
    18901 attagggact ggtaatatca gtatgtccgt ctatgattta aatacgctgt ttacacaagt
    18961 tttatctgga aaactagttt ccaaagactt actcttctca ttatggcgtg aaaatgcgaa
    19021 tggacatcca tatagtggtg gggtatatag cggagataat tatatcctgt cgcaaggaaa
    19081 tatcgactct tttgatacat ctgctatatt tagcaaagat tcacaggatg ctatcatcat
    19141 ggaaagcaat ataaaagctg acaaaactat caaactgcct actgctgatc taagaaacca
    19201 aatctatgac ctgatggaag gaactactaa gttggcaaat agctgatatg atgaaaagat
    19261 agcaaatact acttctttga atagcatttg cttaaaaaaa gtactcgact aaacgtagga
    19321 aagagtgtgg gacaaatcta aaaatcaatt tttgctacag actttaattc caaataaaca
    19381 gaacagaaag aggtgttctt ctttctgttc tgtccaaatt attctaagat ttctctttac
    19441 caacattatt tgacaaaggg cctattaaat ctaaaatagt atctttagat tgctaaaata
    19501 tgtgctagca aatcattcat ggcttgttct tatttttaat tttatgcaat aaaaaagaag
    19561 aacaattagc gtgattatag aaataaataa tgagatttta gaaaaaaatg tgtatgaata
    19621 cctgattgtt actttattta ttccactttc actttttact gtaacaaaag gacctccttg
    19681 ttttagtact gtagcatttt ttgaattaac agtaaccgtt aatcctttat accggaatac
    19741 aggtaaaact attgttttgc cttttgaaga tttaaacgta aatgtaattt catctttatt
    19801 tggagaaaag ctactataaa taacttgacc atcttctgta acttgatggt ttttcaaaag
    19861 caattgatct tttttatagc ctataggtac ataatcaaaa ttctcaaatt cttcaagttt
    19921 attttccatt tgttcattag aataagttcc ttgaaaattc atttctttca ccatgttaac
    19981 tactgatgaa taatttaaaa ttgaaatagc taatattaat aatagtataa aatttcttct
    20041 ccttgtataa gaaaattttt ccaactgtgg agaaataaat agagatactg aaaaagatat
    20101 gaacaatgtt gaaaatatca taaaacgcca aggagcttga ataatattta aaggagtatt
    20161 ttgtaaggca aaccatggaa atatttttgt acaaagtaga atagatatta tacccattat
    20221 ataagtgatt ttagataaga ttcctaattt tttaaagtta aatgttagta taattaaaca
    20281 aataagcaaa gttaagccaa taccaaatga tagtaattct gaatctatac ttccaatcaa
    20341 aaactcttta cccagataaa ttgctctttt ttgtaatata tccacataag gtctattcag
    20401 atcttgatac aatgtctgct caatcatagg acctataaac gctagtgaaa gaagtaaagt
    20461 aactatagca gcttttatta atttaataat taagaagatt tctattttct ttttccgata
    20521 aaattgaata attaaagcaa ttataatcat taccatagtc atagctacag aaagaacatg
    20581 agtgtaaatt aataaagtca tgctaatcgc caacataacc caagattctt tttttgagtc
    20641 gtaaagcttt ataaacccga gtaaaataag aggaagtatt gacattgcga gtaattcccc
    20701 cattgcccct cgaagaaata tttctacact cctaaaagaa gatgttgtat atataatagc
    20761 gaaaaaggag ctagtaacat gttttttggt aatttcatac attacataat gacatataaa
    20821 tagagtaatg agcgtaacaa tataaagaaa aaggttatag cctattgcta aattagtaaa
    20881 cttgatcaat aaaaataaag gatatagagt taaccatggg taaaaattat ttacttgcat
    20941 gccatatgat ctaaacgttt caaaattaat aggggagcta aaaatagatt ctaaacttaa
    21001 cgcacgatta atgtgaaatg ccgtatcata aaagccaaat atttccatgt ttttaaatgc
    21061 tggaaataaa tataataaac taacaaatgc ataaactata aatattttta cagaaaaaat
    21121 ttttttcaaa ttcatatacc acctcatgtt tttttatatt acatatagta tcatttattt
    21181 tttttaaaca ctagattagt cactatataa aatgtgatta attttattat taataaaata
    21241 ttttttatat tacaaaagta gtgttt
//
LOCUS       DASHHB010000046        21243 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.047, whole genome shotgun sequence.
ACCESSION   DASHHB010000046 DASHHB010000000
VERSION     DASHHB010000046.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 21243)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21243)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21243
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.047"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            131..505
                     /locus_tag="U2K71_001974"
     CDS             131..505
                     /locus_tag="U2K71_001974"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster biosynthesis family protein"
                     /protein_id="HEM7316026.1"
                     /translation="MYLKITEQAQERLQKYIDEGATVILDLDDGVGEYSKMGVCSLDT
                     SFRLLLLDKEQRKNDYKLQLDSDIGDVYIKDYSKMYMDEHMTLSLDPRLGVFTLESPS
                     GSLDSHVQLVDLRPEKFSGIKQ"
     gene            complement(619..1248)
                     /locus_tag="U2K71_001975"
     CDS             complement(619..1248)
                     /locus_tag="U2K71_001975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HEM7316027.1"
                     /translation="MKIVFLHGLGQDEQSWKDVISYLPNSYSCKSLSLFNHLNSTDTA
                     TLDDLTKYIEVQLNQIKEPFILCGLSLGAVIALNYLATPSPYLKKVVLVAPQFESPNR
                     LLLTFQNIYFYCVPSKIFEKLGLSKKQTLTLLRSLETLDCKKQLRTSRLPKLILCGTK
                     DHANKPAAKRLYKLSTNGRLLFIKNGKHELNREKPEIMAQAMRSFIENS"
     gene            complement(1382..1454)
                     /locus_tag="U2K71_001976"
     tRNA            complement(1382..1454)
                     /locus_tag="U2K71_001976"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1419..1421),aa:Thr,seq:ggt)
     gene            1668..1741
                     /locus_tag="U2K71_001977"
     tRNA            1668..1741
                     /locus_tag="U2K71_001977"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1702..1704,aa:Asn,seq:gtt)
     gene            complement(1879..2043)
                     /locus_tag="U2K71_001978"
     CDS             complement(1879..2043)
                     /locus_tag="U2K71_001978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316028.1"
                     /translation="MKNLIAGGFLFVGGGILMNTDNFGNVIPFLGAACALIGVGMLIY
                     FVFSDDGKYG"
     gene            2338..3666
                     /locus_tag="U2K71_001979"
     CDS             2338..3666
                     /locus_tag="U2K71_001979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="C1 family peptidase"
                     /protein_id="HEM7316029.1"
                     /translation="MKAITPDMTTKFNKQFQANIKQQALQRAVVKNGITASAENVSAH
                     VENTPVFSIDLATGKVANQMQSGRCWMFAALNTFRHKMLNQFHLKEFELSQNYTFFWD
                     KYEKANYFYENIIATGKEAIDSRKVAFLLATPQQDGGQWDMIVSLFQKYGVVPKSVMP
                     ESANSSNSRDLNNYLNKLLRKDAVVLRKMVAQGETLDAIEEKKEEMLESIYNFLAISL
                     GTPPKEFDFEYRDEEKNYHLDRGLTPQIFYDKYIGVKLDDYVSVINAPTKDKPFNRSY
                     TVEMLGNVVGGKEVKYLNVDMETFKKLAIAQLEEGESVWFGCDVGQSSTRTSGIMALD
                     AYSMDDLFDTDFTMTKAERLDFGESLMTHAMVLTGVDLVDGQSTKWKVENSWGEKVGK
                     NGFFVMSDAWMDEYTYQIVVRKDLLTKEQLNAFNEEPIVLSPWDPMGALA"
     gene            3868..4701
                     /locus_tag="U2K71_001980"
     CDS             3868..4701
                     /locus_tag="U2K71_001980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008266492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructosamine kinase family protein"
                     /protein_id="HEM7316030.1"
                     /translation="MDIQTVLSDLKLNGKVIPVVGGDVNQTYRIKTEHRAYFLKIHPN
                     VKKGFFEAEVDGLKELSAFVRVPDTYMLGETSEGAYLLMEWIEPGKGDQRDLAAALAN
                     LHQQTAPQFGFRKDNYLGTLVQKNSFEEDWWTFFFKDRLESQISLAEETNRWNVQRQE
                     KYLRFKERVLKSVEPKKITPRLLHGDLWSGNVFFDQQGHPVFVDPAVSYGNREQDIAM
                     SQLFGGFRPEFLDAYQTIFPLEKGWKDRLPIYQLYYLLAHLNMFGESYGSQVDQLLEN
                     F"
     gene            4717..5454
                     /locus_tag="U2K71_001981"
     CDS             4717..5454
                     /locus_tag="U2K71_001981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-[acyl-carrier-protein] thioesterase"
                     /protein_id="HEM7316031.1"
                     /translation="MAKKYTKKHEVSYYECDINQTMTFPSMLGVVIKTSEDQSDQLGR
                     GSEFVNSFGLTWVITNYSMEINRLPRVGEVISVTTQAMEYNKFFCYRNFWIHDEDGKE
                     LVKIESVFVLMDFVNRKMSSVNEEIIAPFESEKIKKIKRQEKIEKIDSGFMLPYRVRF
                     YDIDSNQHVNNAMYFNWIIDVLGYDFLTTHVPEKVIIRFDKEVEYGNEIESHFEQIPI
                     ENGVKTKHEIRMQEQLYCEANIIWKNK"
     gene            complement(5609..6577)
                     /locus_tag="U2K71_001982"
     CDS             complement(5609..6577)
                     /locus_tag="U2K71_001982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HEM7316032.1"
                     /translation="MKKILVLHTGGTIAMKENMLTGGVSPDVANPLLDAEITIPKHVE
                     LLVEDIFNLPSPHIMPSHMLVLKERILSAYASGISGVVITHGTDTLEETAFFLDTTIG
                     NKLPIVLTGAMRSSNELGSDGLYNFESAIRVASCEEALDKGVLVVMNDEIHSARYVTK
                     THTTNVATFRTPTLGPIGLVTKNRILFLQELLETKRLDISAVDGTIPIVKAYAGMQGD
                     LLEAIAHTKVDGLVIEALGAGNLPPQALAALEKLLVQKIPVVLVSRCFNGIAEPVYDY
                     EGGGKELEKMGIIFCNSINSQKARLKLLIAVNYGLSGEELISFIQN"
     gene            6809..7642
                     /locus_tag="U2K71_001983"
     CDS             6809..7642
                     /locus_tag="U2K71_001983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HEM7316033.1"
                     /translation="MIRIGLTSFKEHDKLTGKKNNTLYEYAGFLPLVELDTAYYGIPR
                     KSSVENWISEVPESFRFVVKVYQGISGQGDWKPYYQTEEEMIAHFLDVMSPMVDAGKM
                     FCFLVQFPSSFKCTKENVQYLRKLRKWFEGYPVSIELRDYSWYSDEFLERTRNFMSRA
                     AFSLAIIDEPQLLNTTIPFDPFVTNPNFALFRFHGRNAQGWQANDKEWRKKRTLYRYS
                     EKELQELAHEVKVVDSKTKEVGVIFNNNSGGDAAENALALQKILNIKPDGLNPTQMDL
                     F"
     gene            7676..8515
                     /locus_tag="U2K71_001984"
     CDS             7676..8515
                     /locus_tag="U2K71_001984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM7316034.1"
                     /translation="MIKAIFFDIDGTLVTNRSKALESTKQAIEYARKNGILCGVATGR
                     SPVKIKEIIDELELDMYVVYNGQLVFTADRTIIDHPFEQKVLEHIVEFADENHRQIVF
                     GARNRLDGSTTMLLGQSIFIKRLVSFLPRKFPVRLMKKILQVFSPHRQKDRYETLEIL
                     KEPIYQCILLSPESEQKKLEHRFPECTFQRSNTYTVDIIPKGGSKLLGIQAFANAVEI
                     EMEEIMAFGDHYNDIEMLKGVGIGVAMGNAQIEVKHAADFVTQSNEEDGIYEGLKHFG
                     LIY"
     gene            8541..9101
                     /locus_tag="U2K71_001985"
     CDS             8541..9101
                     /locus_tag="U2K71_001985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HEM7316035.1"
                     /translation="MEAETPFQKVETFHKTFDPRRPEIPTAFTENEAHTRAAFKIEEL
                     VEFVAATSPNKEQLHEAVMLLHEALDKAEEKILGKTQWGSDPLVEQVDALTDILYFTY
                     GSFSLLGIVPDRIFEIVHQANMGKLFPDGKPHYDPVTNKVLKPENWQMEYAPEPKIAA
                     EIQRQIQEAIAKKKSADIGMCTNEKE"
     gene            9300..11021
                     /locus_tag="U2K71_001986"
     CDS             9300..11021
                     /locus_tag="U2K71_001986"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septation ring formation regulator EzrA"
                     /protein_id="HEM7316036.1"
                     /translation="MGNNVIIGIVIAIIIVAIVVFLVSYLMKKKNQDRLDALEKRKEE
                     LFDLPVIEEVDDVKKMHLVGQSQNTFREWSQKWNDISTSSFAELESQIFEVENLNESF
                     RFLKGKKAVVEAEATMDQMEKQVTEIREGLKELRETEERNSLAVQQALDVYEELKEKV
                     REDEHEYGPALFELQKQIKVIEKEFTQFVTLNTSGDPVEAREVLDKAEKHTYEVEALM
                     KKIPPLYEELHTTFPEQLEEISDTYEKLMEEQYVFPEEDLAEDIAKVSRRIENSLANL
                     EKTEVETVEFENRETADLIDSLYDILEREMEAQRYVKTNQSTIAEYIKHTTKNNRQLL
                     IELDHTSQSYTLNHNEIGRVRGFQTEVEEMERQNEQMIPQIRQHEIPYSEVRTFYKTV
                     FKVLEDIEKQQVEIDDSLHELRKGEKEAQEKIDTFEFKLRSLKRFVEKQRLPGLPNDY
                     LEFFFVATDRIEELSVVLNKIRVNMEEVNRLVALCEEDLELLDKKTHDLVDAAALTEQ
                     MLQYANRYRHTHEEVREAIDKSYYLFNKEYHYQEALDEIGTALERVEPGAFKRIEDFY
                     FNHPDLV"
     gene            11186..12328
                     /locus_tag="U2K71_001987"
     CDS             11186..12328
                     /locus_tag="U2K71_001987"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HEM7316037.1"
                     /translation="MIYFDNSATTPIFPQSLDAYVKTSQRIIGNPSSLHDLGNQANRL
                     LQQARKQIADLIHVEPEEIYFTSGGTEGDNWVLKGTMIEKREYGNHMIISGVEHPAVS
                     ETAEQLKELGIEVSIAPVDKRGFVRVDELKELIRKETVLVSVMAVNNEVGSIQPIQEI
                     SDLLDDFPKIHFHVDAVQAIGKVPFSQWLTERVDFATFSAHKFHGPRGTGFIYWKKGR
                     RLAPLLTGGGQERNQRSGTENVPGIVAMVKALRLSLEIKEQKPEQTQRLKVALINALK
                     TYEKVTIFSEGTDYAPHILCFALKGIRGEVLVHALEEKQIFVSTTSACSSRKHMASST
                     LHAMHVPNDLATSAVRISLDENNSLAEVEQFMIIFKQLYKKFSAIN"
     gene            12344..13555
                     /gene="thiI"
                     /locus_tag="U2K71_001988"
     CDS             12344..13555
                     /gene="thiI"
                     /locus_tag="U2K71_001988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 4-thiouridine(8) synthase ThiI"
                     /protein_id="HEM7316038.1"
                     /translation="MKYSEIMVRYGELSTKGKNRKSFIMQLAQNVRQALKDFPEIKIH
                     ADRDRMHLLLNGADGQLVIPKLAKIFGIQNFSPSIRVEKDVEVLKQAVQDIMKEIYTG
                     KETFKIIAKRSDHQFELTSNELNQTLGNAVFDIFPHIQVQMRQPDIPLRVEIRRDGAY
                     LSYETIKGAGGLPVGTSGKGMLMLSGGIDSPVAGYFAMKRGVEIEAVHFASPPYTSEQ
                     ALQKAKDLTAKLAPYVGTIHFIEVPFTEIQEEIKKSVPQGYWMTITRRMMLRLTDEIR
                     RIRHGLVILNGESLGQVASQTLQSMVAINEVTNTPIIRPVATMDKLEIIEVAEQIDTF
                     NLSIQPFEDCCTIFAPPQPKTRPQLEKVHQYEERLAIDEMIQRALAGLKMEEIQAESS
                     NEKTEEFSELL"
     gene            14128..14777
                     /locus_tag="U2K71_001989"
                     /pseudo
     CDS             14128..14777
                     /locus_tag="U2K71_001989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341217.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
     misc_binding    14906..15122
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            15170..17815
                     /locus_tag="U2K71_001990"
     CDS             15170..17815
                     /locus_tag="U2K71_001990"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HEM7316039.1"
                     /translation="MTEENNLSTKYNPQEVEAGRYQHWLEQDLFKPSGDKKAKPYSIV
                     IPPPNVTGKLHLGHAWDTTLQDMLIRQKRMQGYDTLWLPGMDHAGIATQAKVEAKLRE
                     QGISRYDLGREKFIDQVWEWKEEYASHIREQWAKMGLSLDYSRERFTLDDGLSEAVRK
                     VFVTLYEKGLIYRGEYIINWDPQARTALSDIEVIHKDIEGAFYHMSYELADGSGVVEI
                     ATTRPETMLGDTAIAVHPEDERYQALIGKKVILPLVNKQIPIIADEYVDMEFGTGVVK
                     ITPAHDPNDFEVGNRHDLPRVNVMDDDGTMNELAGKYAGMERFAARKAIVQDLKEIGR
                     LIKIDTMIHSVGHSERTGVVVEPRLSTQWFVKMAPLAKKAIDNQETDQAVEFFPPRFN
                     QTFLRWMENVHDWVISRQLWWGHQIPAWYHKETGEMYVGMEAPADSENWVQDPDVLDT
                     WFSSALWPFSTMGWPDTESEDYKRYFPTSTLVTGYDIIFFWVSRMIFQSLEFTGERPF
                     ENVLIHGLIRDEQGRKMSKSLGNGIDPMEVIEKYGADALRWFLSNGSAPGQDVRFSYE
                     KMDASWNFINKIWNASRFVIMNIEGMTVEEIDLSGEKSVADRWILTRLNETIERVTEL
                     FDRFEFGEAGRQLYNFIWDDFCDWYIEMSKEVLYGDDAVSKQTTRSILVHTLDQILRL
                     LHPIMPFVTEEIWEHIPHQGNSLVVAEYPVVRPEFDDETASKGMEVLKELIRAVRNIR
                     SEVNTPLSKPITLLIKTNDAKIDQFLVENTSYIERFCNPEELTISSEITAPDLAMSAV
                     LTGAEIFLPLAGLINIEEEIKRLEKELEKWTSEVKRVQGKLSNERFVSNAPEEVVEAE
                     RAKEKDYLEKQTAVKERIESLRTIQ"
     gene            18125..19438
                     /locus_tag="U2K71_001991"
     CDS             18125..19438
                     /locus_tag="U2K71_001991"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional folylpolyglutamate
                     synthase/dihydrofolate synthase"
                     /protein_id="HEM7316040.1"
                     /translation="MTIEEAIEWIHSRLPFGSRPGLDRVEALLEVVGHPERKVKTIHI
                     AGTNGKGSTVAYLRSLLEEAGLTVGTFTSPYIETFNERIAINGKSIPDNDLIQLVKKY
                     QPLIKQLDQFEAVAGITEFETLTAMAFDYFLMKKVDVAIIEVGLGGLLDSTNVAVPML
                     TGITTIGLDHTEILGDTIEEIAAQKAGIIKQGVPVVTGNIAPEAFTVIQQTAKAKNAP
                     LYVYGENYKINYLHPDEMWGEVFEFFNEIGKIPQLTTPLLGQHQAENAGVAIELYFVY
                     CQLEKLPFREKDVLKGLKKSRWPGRMERLSNEPLVVLDGAHNTHAMNRLVENLKKEFK
                     GYQINILFSALRTKNVREMLERLQQVPNVHIYLTTFEHPKAIELSEVSDLASERVSIV
                     SLWQFGLADILEKMTNEDVLVVTGSLYFISEVRHLLIQLGGDHEV"
     gene            19428..20081
                     /locus_tag="U2K71_001992"
     CDS             19428..20081
                     /locus_tag="U2K71_001992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM7316041.1"
                     /translation="MKYNAVIFDMDGLLFDTELVYYEATQVVADKMGFPYDKDLYLKF
                     LGVSDEEVWANYHMIFSEFGKTSVQRFIDDSYQETLERFSQGAVQMKPGVLELLSFLE
                     EHQIPKVVASSNQRAVIELLLKKNNLFERFETIVSAENVQRAKPDPEIFLLANQKLGT
                     AKKETLILEDSQNGILAASAAEIPVVMVPDLLPPSQELIKKTAGVVSSLHEIPALLK"
     gene            20136..20828
                     /gene="radC"
                     /locus_tag="U2K71_001993"
     CDS             20136..20828
                     /gene="radC"
                     /locus_tag="U2K71_001993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HEM7316042.1"
                     /translation="MGKRLIKEVPTSSLPRERMEIYGAEALSDQELLAILLRTGQHPY
                     SVMSIAGNLLKTFGGLASLRQATLHELEEIRGIGRVKAIEIKALIELGRRIQTDHERA
                     APPVRTSYELAQQLILEMKDHKQEHLVCIYLDTKNQVILKKTVFIGSLNQSVAHPREI
                     FHYAVRYCAARIVLAHNHPSGNVIPSQQDMNFTKRIQKCGEMMGITVLDHLIIGRKRY
                     FSLREEGMMEEK"
     gene            20978..21178
                     /locus_tag="U2K71_001994"
     CDS             20978..21178
                     /locus_tag="U2K71_001994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003234247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HEM7316043.1"
                     /translation="MENGTVKWFNAEKGFGFISREDGSDVFVHFSAIQGDGFKTLEEG
                     QAVTFDVEESDRGPQAANVVKA"
BASE COUNT         7173 a         3542 c         4438 g         6090 t
ORIGIN      
        1 ctacactctg agatgtaaga aatatcataa gatatttctt acatcttttt gttttgagca
       61 ggactttaga taattttatt aatatatgct agcatgcttt tgtaagaaaa agaaagaggg
      121 cgtattagtt atgtatctta aaattacgga acaagcacaa gaaagattac aaaaatatat
      181 tgatgaaggc gcaacagtta tattggattt agatgatggc gttggggaat attctaaaat
      241 gggtgtttgc tctttagata ctagttttcg tttacttctt cttgataaag aacagagaaa
      301 aaatgattat aaactccaat tagatagtga tattggggat gtttatataa aagattattc
      361 aaaaatgtac atggatgaac atatgaccct ttctttagat ccacgtctag gtgtatttac
      421 tcttgaaagc ccaagtggtt ctttagacag tcatgttcaa ttagtagatc tacgaccaga
      481 aaaattctct ggaataaaac aataactttc tctctattgt ccataaaact agtctcaatc
      541 aagctgtatt gaaaaaacaa gacaacattc ttaccatggt aaataaaaag acgacacatt
      601 ttgtcgtctt tttttaaatt aactattttc gataaaagat cgcatagctt gtgccattat
      661 ttctggcttt tctctattca attcatgttt tccgttttta ataaagagta gccggccatt
      721 cgttgaaagt ttatataaac gttttgctgc tggtttattc gcatgatctt tggtaccgca
      781 taaaatcaat ttaggaagtc gtgatgtacg aagctgtttt ttgcagtcta atgtttcaag
      841 tgaacgaagt agggtaaggg tttgtttttt agatagacct aatttttcaa atatcttaga
      901 aggtacgcag taaaaataaa tattctggaa agtaagcaat aagcgattag gagactcaaa
      961 ttgtggagcg actaaaacaa ccttttttaa atatggagaa ggagttgcaa gatagtttaa
     1021 agcaataaca gcgcctaaag ataatccaca taaaataaaa ggttccttta tctgattcaa
     1081 ttgtacttcg atgtatttgg ttaagtcgtc taaagtagca gtgtctgtgc tgtttaaatg
     1141 attgaacaat gaaagacttt tgcaagaata actattgggt agataagaga ttacatcttt
     1201 ccatgattgt tcgtcttggc ctagaccatg caaaaaaaca attttcatat tgctctcctt
     1261 tttatagtta tcatatagac tttctttgta ctattaaaat ataaacattc atttcttacg
     1321 tcataaaata aaaaaacagc gcagacacat tgttaaacca acatgttgca ctgtttaagg
     1381 aagccacttg ccgggcttga accggcgacc tcttccttac catggaagcg ctctaccagc
     1441 tgagctaaag cggcattatc attaatacaa ataataggat aacgaatatt aatgtatttg
     1501 tcaacagaat tttttcgaaa aaatgtgttt ctctaatagg aaaacagatt ttagaaaaaa
     1561 taaaaataac aaaaaatgcg aacggtttct ttgataatat aataaaaaaa ttcaaaaaaa
     1621 gtattgcgtt atttagaaga aattgatatt atattcatgt acttgattcc ggcatagctc
     1681 agttggtagt agcgcatgac tgttaatcat gatgtcgtag gttcgagtcc tactgccgga
     1741 gtaaataaag cttatgcaaa gaaaaagaaa tccaatcact tgggtttctt tttcttttat
     1801 tattatgatg aatcaagctt tctgtttttg tcacgtatgt tgtaatcttt acgaaagagt
     1861 agagttggct actcataatc aaccatactt cccatcatcg ctaaatacga aataaatcag
     1921 cattcctaca ccaatcaaag cacaagctgc accaagaaaa gggatgacat tcccaaagtt
     1981 atctgtattc ataagtatgc caccacccac aaacaaaaag ccgccagcaa tcaggttttt
     2041 cattttattc atctcctttc ttacgttata gtatttcctt ctttcaatga gtattttgaa
     2101 agctatctta ttgtatgatg ttcttctagg cctcttcttt attatacagg ccattttcgt
     2161 tatctcatag aaatattcct ttttatctga aaactattgt ataccgtatg acatttttct
     2221 tcatgaagtt gtaagtatat tctcattcaa atgaaaattt catcatagaa gagattgtat
     2281 ttctaaattc ttaagagtaa aatacgaagt ataacttgga tttggaagga tgattggatg
     2341 aaagctatta cgcctgatat gacaacaaaa tttaacaaac agtttcaagc aaatatcaaa
     2401 cagcaggcat tgcagcgggc agttgtgaag aacggaatta ctgcttctgc tgaaaatgtt
     2461 tcagcacatg tggagaatac accggtattt tctatagatt tggctacggg gaaagtggct
     2521 aatcaaatgc aatcggggcg ttgctggatg tttgctgcac tgaacacatt ccgtcataag
     2581 atgctgaatc aattccattt aaaagagttt gaattatcac aaaattacac ttttttctgg
     2641 gataaatatg aaaaggcaaa ttatttctat gaaaacatca ttgcaacagg aaaagaggca
     2701 atcgatagcc ggaaagtagc tttcttactc gctactcctc aacaagatgg cggccaatgg
     2761 gatatgatcg tttcattatt ccaaaaatat ggcgttgtgc ctaaatcggt gatgccggaa
     2821 agtgcgaata gttcaaattc tcgagatttg aataactact tgaacaaatt actgcgtaaa
     2881 gatgcagtag tgttacggaa aatggttgct caaggagaaa cattagacgc tattgaagaa
     2941 aagaaagaag aaatgctaga aagcatttat aacttcttag ctatctcatt aggaacacct
     3001 ccaaaagaat tcgattttga gtaccgcgat gaagaaaaga actatcatct tgaccgagga
     3061 ctgacaccac aaatttttta tgataaatac atcggtgtaa aattagatga ttatgttagc
     3121 gtgatcaatg caccaacaaa agacaaacca ttcaatcgtt cttatacagt agagatgcta
     3181 ggaaatgtcg ttggcgggaa agaagtcaaa tatctgaatg ttgatatgga aactttcaaa
     3241 aaattagcga ttgctcagct tgaagaagga gaatctgttt ggtttggttg tgatgtaggt
     3301 caatcttcta ctagaactag tggcatcatg gccttagatg cttattcaat ggatgatcta
     3361 tttgatacgg attttacaat gactaaagct gaacgtttag attttggaga aagtctgatg
     3421 acccatgcga tggtattgac cggtgttgac ttagtagacg ggcaatcaac aaaatggaaa
     3481 gtcgaaaata gctggggcga aaaagttgga aagaatggct tctttgttat gagtgatgcc
     3541 tggatggacg aatatacgta ccaaatcgtt gtaagaaaag atttattgac gaaagagcaa
     3601 ttaaatgcgt tcaatgaaga accaattgtt ctatctccgt gggatccaat gggtgcttta
     3661 gcatagacga gtatttcaat gaagagtatg tagtaaagat aaaaagagct ggcttaagcc
     3721 agctcttttt atctttaaag tccattccct gttttttatg gtccctgtgc tataattcta
     3781 aataaaggat aaggattgga aataatcctt ttatcattaa aaatatgatg acgaagtaga
     3841 ataaaattgg gagtaggggg gaaagtcatg gatatccaaa ctgttttatc tgatctgaaa
     3901 ttaaatggta aagtgattcc agttgttggt ggcgatgtta atcagaccta tcgtataaaa
     3961 accgaacatc gtgcttattt tttaaaaatc catccgaatg taaaaaaagg attttttgaa
     4021 gcagaagtag acggattaaa agaactgtca gcattcgtac gtgtaccgga tacgtatatg
     4081 ctaggtgaaa caagtgaagg cgcttatttg ttaatggaat ggatcgagcc gggaaaaggg
     4141 gatcaacgag atcttgctgc agctttagct aatctccatc agcaaacagc gccacaattt
     4201 ggttttcgta aagataacta tttgggtaca cttgttcaga aaaattcttt tgaagaagat
     4261 tggtggactt ttttctttaa agatcggctt gaatcccaga tttctttggc agaagaaacg
     4321 aatcgatgga atgtacagcg tcaagaaaag tatctgcgat ttaaagaacg tgtattgaaa
     4381 tcggttgaac caaaaaagat cactccacgc ctcttgcacg gagacctgtg gagtggtaat
     4441 gtgttctttg accagcaagg gcatccagtt tttgtcgacc cagctgtttc ttatgggaat
     4501 cgggaacaag atattgcaat gagccagtta ttcggtggtt tccgaccgga atttttagat
     4561 gcttatcaaa ctatatttcc attagaaaag ggatggaaag accgactgcc gatttaccag
     4621 ttatattatc ttttggcaca tttgaatatg tttggagaaa gttacggttc ccaagtagat
     4681 caattactag aaaattttta agaggagggc gtgtttttgg caaaaaaata taccaaaaag
     4741 catgaagttt catattatga gtgtgacatc aaccaaacga tgacatttcc ttcgatgtta
     4801 ggagtagtta tcaaaacgtc tgaagatcaa agcgaccagt tgggaagagg cagtgaattc
     4861 gtcaatagct ttggacttac ttgggtcatc acgaattatt ccatggagat taatcgtctc
     4921 cctagagtcg gtgaagtaat ctcagtaacg actcaagcca tggagtacaa taagtttttc
     4981 tgttatcgga acttctggat ccatgatgaa gatgggaaag aattagtcaa aatagaatct
     5041 gttttcgtat tgatggattt tgtgaatcga aaaatgagca gtgttaatga agagatcatt
     5101 gctccttttg agagtgagaa gatcaagaaa atcaagcgtc aggaaaaaat tgaaaagatt
     5161 gattcgggct tcatgttgcc atatcgtgtt cgtttttatg atatcgatag taatcagcat
     5221 gttaataatg cgatgtattt taattggatc attgacgtat taggatatga ctttttgact
     5281 acgcatgttc cagaaaaagt aattattcga tttgataaag aagtagagta cgggaatgag
     5341 atcgaaagcc attttgaaca aatccctatc gaaaatggtg tcaaaacaaa acatgaaatc
     5401 cgaatgcaag aacagctgta ttgtgaagca aatattatat ggaaaaataa ataaaaaaga
     5461 ggttgtgaca gaagggggca acttcaagaa ataagaagaa atttacgaaa attgtttctc
     5521 aaatttttgt aaattttcga cttagttttc gaagaagcta tttttgtcac agcctctctt
     5581 tttaagttta gctattagat aatgatgttt agttttggat aaacgaaatc aattcttcac
     5641 ccgacaaacc atagtttaca gcaatcaata gttttaaacg tgctttttga ctattaatac
     5701 tgttacagaa aataatcccc attttttcta gttcttttcc cccgccttca taatcataaa
     5761 caggctctgc gattccattg aaacaacgag aaacaagtac tacagggatt ttttggacaa
     5821 gaagcttttc caaagcagct aaggcttgtg gagggagatt ccccgcacct aaagcttcga
     5881 ttaccaaacc gtctactttc gtatgggcga tggcttccaa taagtcccct tgcatcccag
     5941 catatgcttt aacgatcggg atagtcccat ctacagcgga aatgtccagt cgctttgttt
     6001 ctaaaagttc ctgcaagaat aaaattcgat tcttagttac taagccgatg ggacccaatg
     6061 taggtgttcg gaaagtagca acatttgtgg tatgggtctt tgtcacgtag cgtgcagaat
     6121 ggatctcatc gttcatgacc accaaaaccc ccttatctag cgcttcttca caacttgcga
     6181 cacgaatcgc actttcaaaa ttataaaggc cgtcacttcc aagttcatta cttgaacgca
     6241 ttgccccagt caaaacaatt ggtaatttgt tgccaatcgt tgtatcaaga aaaaaagcag
     6301 tttcttctaa tgtatctgta ccgtgtgtga tcaccactcc ggatatacct gatgcataag
     6361 cggacaagat acgttctttt aataccaaca tgtgagaagg catgatgtgt ggtgagggaa
     6421 gattgaagat atcttcaact aataattcta catgcttggg gattgtgatt tctgcatcta
     6481 ataatgggtt tgccacatca ggactaacac cgcccgtgag catgttttct ttcatggcga
     6541 tcgttccgcc ggtgtgtagt acaagtattt ttttcatttt acagacttcc ttacgaataa
     6601 ttttgcatag ccgttaaatc ttattgtata ctatataaaa gagttttgct atcagtgaag
     6661 aataacgaat agcctaactt atcataaaaa agctgacctt attaaaatgg aaaagactca
     6721 accttgacct cctaatacaa tggagtatct ggttgagttt cttttacaag aaagcagcca
     6781 aggaaatcaa tcacgaaggt ggccggaaat gatcagaatc ggattaacat cgtttaaaga
     6841 acatgacaaa ttaactggta aaaagaataa cactctttat gaatatgcag ggtttctacc
     6901 cttagtggaa ttagatactg cctattatgg aattccccga aagtcctcag ttgagaattg
     6961 gataagtgaa gtacctgaat catttcgctt tgtagtcaaa gtctatcaag gaatcagcgg
     7021 tcaaggagac tggaagcctt attatcagac cgaagaagag atgattgcgc attttttaga
     7081 tgttatgtcg ccaatggtag atgctggaaa gatgttttgc tttttagtcc aattcccctc
     7141 atcattcaaa tgtacgaaag aaaatgtcca gtacttgcgc aaacttagaa agtggtttga
     7201 gggttatcct gtttctatag aattgcggga ttattcttgg tacagcgatg aatttcttga
     7261 acgtacaaga aactttatgt ctagagccgc tttttcttta gccatcatcg atgagccaca
     7321 gttgctgaat acaacgattc cttttgatcc atttgtcacc aatccgaact ttgctctttt
     7381 tcgattccat ggtaggaatg cgcaaggatg gcaagcaaat gataaagagt ggcgtaaaaa
     7441 aagaacgtta tatcgttatt cagaaaaaga actgcaagaa ctagcacatg aagtcaaagt
     7501 agttgattct aagacaaaag aggtaggtgt gatttttaat aataattctg gtggtgatgc
     7561 ggctgaaaat gcattagcac tgcaaaaaat attaaatatc aagccagatg gactcaatcc
     7621 aacccaaatg gatctgtttt aagggcttat tttattcaag aaaggggcga agtctatgat
     7681 caaagctatt ttttttgata ttgacggaac attggtcacc aatcgttcaa aagcgttgga
     7741 atcaacaaaa caagcaattg aatatgctcg gaaaaacggc attttatgcg gtgtagcaac
     7801 ggggcgcagc ccagtaaaga tcaaagaaat cattgatgaa ttggaattag atatgtatgt
     7861 cgtgtataat ggtcaactgg tttttactgc agatcgcacg attatcgacc acccttttga
     7921 acaaaaagta ttggaacata ttgttgaatt tgcagatgag aaccatcgac aaatcgtttt
     7981 tggtgcgaga aatcggttag atggatcaac gacgatgcta ttaggacaat cgatttttat
     8041 caagcgacta gtcagctttt taccgcgtaa attcccggta cgtctgatga aaaaaatcct
     8101 ccaggttttt agccctcacc gacaaaagga tcgttatgaa acgttagaga tattaaaaga
     8161 accaatttat caatgtatat tgctcagtcc ggagtcagaa cagaaaaagt tagaacatcg
     8221 ctttcccgag tgtacgtttc agcgttcaaa tacgtatact gtagatatca tcccaaaagg
     8281 tggttcaaaa ttactaggca tccaagcttt cgcaaatgct gtcgaaattg aaatggaaga
     8341 aattatggca ttcggcgatc attacaatga tatcgagatg ctgaaaggtg taggaattgg
     8401 agtggccatg gggaatgctc agatcgaagt aaaacatgct gcagactttg tgactcaatc
     8461 aaatgaagaa gatgggatat acgaaggact gaaacacttc ggtctgattt attagtcaat
     8521 gaaagaagga aaaaatcagt atggaagcag agacaccttt tcaaaaagta gaaacattcc
     8581 acaaaacttt tgatccaaga agaccagaga tacccacggc ttttacagaa aatgaagctc
     8641 atacacgtgc tgcgttcaaa atagaagaac ttgttgaatt tgttgcagct acttcaccta
     8701 ataaagaaca actgcatgaa gctgttatgc tgctgcacga agcattagat aaagcagaag
     8761 aaaaaatact tggtaaaaca cagtggggaa gtgatccgct ggttgaacaa gtagatgcac
     8821 tgacagatat cctttacttt acttatggtt ctttttcatt actaggaata gtgcctgatc
     8881 gaatattcga aatcgtccat caggcaaata tggggaaatt atttccagat ggaaaacctc
     8941 attatgaccc tgtaacaaat aaagtgttga aacctgaaaa ctggcagatg gaatatgctc
     9001 cagaacctaa aatcgcggca gaaatccaac gtcagattca agaagcgatt gctaagaaga
     9061 aatccgctga tataggcatg tgcacaaatg aaaaagagtg atcagagttt cactattttt
     9121 gttatcttta tacgatggaa aaacaaaaga ttttcgttta agttagtgaa tgcatgaaaa
     9181 tagtatgcta taatctagaa tagatgtctt ttgcacgaga gacgtattat ttggatttat
     9241 gccaagcaga acgccatctg ttgcataaaa gatgcatact gaaaacggag gaaccataaa
     9301 tgggaaataa tgttatcatc ggaatcgtca ttgcaatcat tatagtcgcc attgtcgtct
     9361 ttttggttag ttacttgatg aagaaaaaaa accaagatcg attggatgcc ttagaaaaac
     9421 gcaaggaaga attatttgac ttgccagtga ttgaagaagt cgatgatgtt aaaaaaatgc
     9481 atctggttgg gcaaagccaa aatacctttc gtgaatggag tcaaaaatgg aatgacatat
     9541 ctacttcctc gttcgctgaa ctagaaagtc aaattttcga agtggaaaac ctcaatgaaa
     9601 gttttcggtt cttaaaagga aaaaaagcag ttgttgaagc agaagctaca atggaccaaa
     9661 tggagaaaca agtgacggaa atccgtgaag gtctaaagga attgcgggaa acggaagaac
     9721 gcaactcact agctgttcag caggcacttg acgtctatga agaactgaaa gaaaaagtca
     9781 gagaagacga gcatgaatac ggaccagctc tttttgaatt gcaaaaacag atcaaagtga
     9841 tcgaaaaaga atttacgcag tttgtgactt taaacacttc tggagaccct gttgaagccc
     9901 gcgaggtttt ggataaagca gaaaaacata cgtatgaagt ggaagctctg atgaaaaaaa
     9961 ttccaccgct gtatgaggag ctccatacca ctttccctga acaacttgaa gaaatcagtg
    10021 atacttacga gaaattgatg gaagaacaat atgtattccc agaagaagat cttgctgaag
    10081 atatcgcaaa agtaagtcga agaattgaaa attcattagc caatcttgaa aaaacagaag
    10141 ttgaaacggt cgaatttgaa aaccgtgaaa cagctgattt gattgattcg ttatatgata
    10201 tcttagaacg ggaaatggaa gctcagagat atgtaaaaac taaccaatca acgattgcag
    10261 agtatatcaa acatacaaca aagaataacc gtcaattatt gattgagctg gatcacacat
    10321 ctcaaagcta tacattgaac cacaatgaaa ttggccgtgt acgaggtttc caaaccgaag
    10381 tggaagaaat ggaacgtcaa aatgaacaga tgattccaca gatccgtcaa cacgaaattc
    10441 cttattcaga agtgcgcacg ttctataaaa cagtcttcaa agtcttagaa gatatcgaaa
    10501 agcaacaagt agagatcgac gactcattac atgaattgag aaaaggcgaa aaagaagcgc
    10561 aagaaaagat cgatacattc gaattcaaat tgcgcagttt gaaacgtttt gtagaaaaac
    10621 aacgtttgcc tggcttgccg aatgattacc tagaattctt ttttgttgct actgaccgta
    10681 ttgaagaact aagtgttgtc ttgaataaaa ttcgagtaaa catggaagag gtcaatcgtt
    10741 tagttgctct atgtgaagaa gatctagaat tattagataa gaagacacat gatttagtag
    10801 atgcagcagc attaacggag caaatgctcc aatatgcaaa tcgttatcgt catacacacg
    10861 aagaagtgag agaagcaatt gataaaagct actatttatt caataaagaa tatcactatc
    10921 aagaagcatt agacgaaatc ggtacagcat tagagcgagt agaacctggt gctttcaaac
    10981 gcatcgaaga tttttatttt aatcatccag acttagtata aagcaaaaaa gttagtaaac
    11041 cagcaagagc aagacggatt attgtcttgt tcttgtcttt ttttgtatat ttgaaaattg
    11101 aaacaagtga aaatttcacc tgctcttgaa agcaccttgg acaaccgtta taatagaaaa
    11161 gaatttcaaa aaagaggggt ttcttatgat atattttgat aacagtgcaa caaccccaat
    11221 ctttccgcaa agcttagatg catatgtaaa aaccagtcag cggattattg ggaatccatc
    11281 aagcttgcat gatttaggca atcaagctaa ccggttgttg cagcaagcaa gaaagcagat
    11341 cgctgatttg atccatgtag aaccagaaga aatctatttt acttcaggcg gaacagaagg
    11401 agataactgg gtattaaaag gaacgatgat cgaaaaaaga gaatatggga atcatatgat
    11461 catttcagga gtcgaacatc cggccgtcag cgagacagca gaacaattaa aagaattagg
    11521 aatcgaagta agtatcgccc cagtggataa acgcggattt gtacgtgtcg acgaattgaa
    11581 agaattaata agaaaagaga cagtattagt ctctgttatg gctgtgaata atgaagtagg
    11641 atcgattcag cccatccagg aaatcagtga tcttttggat gattttccta aaattcattt
    11701 ccatgtagat gctgttcagg cgattggaaa agtaccattc tcgcaatggt tgacagaaag
    11761 agttgatttt gcaacatttt cagctcataa attccatggc ccacggggaa ctggtttcat
    11821 ttattggaaa aagggtcgtc gtttagcacc attactgaca ggtggagggc aagaaagaaa
    11881 tcagcgaagt gggacagaaa atgttccagg tattgtggct atggttaaag ctttacgttt
    11941 atcattagag ataaaggaac aaaaacctga gcagacacaa cgcttaaaag tagctctaat
    12001 caatgcgttg aagacgtatg agaaagtgac gattttttca gaaggaactg actatgcacc
    12061 gcatatttta tgttttgctc taaaaggaat tcgcggcgaa gttctcgtcc atgccttaga
    12121 agaaaaacaa atatttgttt ctactactag tgcctgctcc tcacgtaaac atatggcaag
    12181 cagtacattg cacgcaatgc atgtaccaaa tgatctggct acatctgctg ttcgaatcag
    12241 cttagatgaa aacaattcgc ttgcagaagt cgagcaattt atgattattt tcaagcagct
    12301 ttataagaaa ttttctgcaa tcaattaaaa gaggagtgtt cttgtgaaat acagtgaaat
    12361 tatggtccgt tatggggaac tttcgacaaa aggaaagaat cgaaaaagct ttatcatgca
    12421 actagcacaa aatgtcagac aagctttgaa agactttcca gaaatcaaaa tccatgctga
    12481 tcgggaccgg atgcatcttt tattgaatgg cgcagacggg cagctagtta ttccaaaact
    12541 agcaaaaata tttgggatcc aaaatttctc tccaagtatt cgtgtagaaa aagatgtcga
    12601 agttttgaaa caagctgttc aagacatcat gaaagaaatt tatactggga aagaaacatt
    12661 taaaattatc gctaaaagaa gcgatcacca atttgaattg acaagtaatg aattgaatca
    12721 aacattaggt aatgctgttt ttgatatttt cccgcatatc caagttcaaa tgcgtcaacc
    12781 agatattcca ttacgtgtag agattcggcg tgatggcgct tatctatcgt atgaaacgat
    12841 caagggagct ggaggattgc ccgtaggaac aagcggaaaa ggaatgctta tgctttctgg
    12901 ggggattgat tctcctgtag cagggtactt tgccatgaag cgaggagtgg aaattgaagc
    12961 tgttcatttt gctagtccgc cgtatacaag tgaacaagca ctgcaaaaag caaaagatct
    13021 aacagccaaa ctagcacctt atgtgggaac gatccatttt atcgaagtgc catttaccga
    13081 gattcaagaa gaaatcaaaa agtcagtgcc acaaggatat tggatgacaa ttactcgtcg
    13141 aatgatgttg cgtttgaccg atgaaattcg ccgtattcgt catggtttag tcattttgaa
    13201 tggtgaatca ttaggtcaag tggcttcaca aactttgcaa agtatggtgg ctatcaatga
    13261 agtaacgaat acgccaatca ttcggcctgt agcgacaatg gataaacttg aaatcattga
    13321 agtagcagaa cagatcgata catttaatct ttctatccag ccttttgaag attgctgtac
    13381 gatttttgct cctccacagc caaaaacacg ccctcaactg gaaaaggtcc atcaatatga
    13441 agagcgttta gcaatagatg aaatgatcca acgagcacta gctggcttga agatggaaga
    13501 gatacaagca gaatcgtcta atgaaaaaac tgaagaattt tcagaattgc tataagaggt
    13561 tatgaaaata gtgttttgac cagagtatta gagaaacaaa acgaaaaaat agctccatgt
    13621 attttaccgt ttttgaacta atgtcgaaga ttagctgatt gaataccatt tatcaagaag
    13681 ttatggaaaa gccggttctt ttttactaac tgaaaaataa aattttattt agagatcaag
    13741 ctacagttga taaatgatat aaaaaagaga atttcccatg aaatagcgag gttgtgacag
    13801 aagtgggcag cttcaagaaa taagaaggaa tttacgaaaa ttgcttttca aatttttgtg
    13861 aaatttcggc ttatttttcg aagaagttac ttctggaaca ccgtgtatca gaggttgcga
    13921 aaaagctgcc ctgcatcaag taataagaac agccaaacaa aaatagttcc tcatatttta
    13981 cgtttggttg agcttattac cgcagatgca agcttttgaa caccgtttta tttgtctcac
    14041 acccttttcc ttcctacttg taaccttgct cccgtgttag aaacatgtta cactaaatat
    14101 gtgaaattat taacaaggag cgtcgttatg cgagaaaaaa cagatacact gccaaaagca
    14161 actgcgaaaa gattaccttt atatttacgc tacttgaaga tgctggatga ctcaggtata
    14221 agtagaatca aatcaaatga gttcagcgaa atcacccaaa ttccttctgc aacgatccgc
    14281 agagacttct cgcaattagg cgagctaggc agaagcggat atggggctat gatgtccctt
    14341 ttcttatcga tgtattcaat aatatcctta atactaaaga agaaaaacga atcgcattag
    14401 taggatatgg aaatcttggc aaagcattga aacacaacaa ttttagaaga aatgaaaatc
    14461 taaatatcgt gtgcgtcttt gataatgatc cagcactgat caatcgagta attgatggag
    14521 aaatgatcta tccgattgat cgtttcgcgg agatagccaa agcaaaaaat gtgacagtag
    14581 caatctctac ggtaccaagt aaatattcac aatctgctat tgatgaaatt gtcaaaggaa
    14641 atgtaaccgc tatacttaat tttgcgcctg acagagtaac tgtcccagct tatgtcaatg
    14701 ttcaatacat tgatttgaca accgagttgc agacgcttat ttactttgat gagaattact
    14761 cagaagtttt ttcttaggaa aatttgagta ggcttattga tcttgcgaga actcgtcgta
    14821 attctttcaa aaatttttta gtactaaatg aagtggtttt ttcacgacca cttgttgact
    14881 aaaaaaaaaa accattgtag aatgaagcat aacgctgaac aagaggagta actggcacat
    14941 tcattcttag agagagaact atctgctgaa aggttctcaa tgacaaccag tgaaggtagc
    15001 ttgggagttt ctgtcttgaa tggagtagag atagacgagt cgtgatcgtt aactcacttg
    15061 agaggcaata atgattgatt attgcaattt aggtggtacc gcgtaaatca tacgtcctag
    15121 aatacgaaag tattttagga cgttttttag tttaaaggag gaaaacagca tgacggaaga
    15181 aaataaccta tcaacaaaat ataatccgca agaagttgaa gctggacgtt atcagcattg
    15241 gttagaacaa gatttgttta aaccgagcgg agataaaaaa gcaaaacctt attcaatcgt
    15301 aatcccgccc ccaaatgtta ctggaaaact tcatttaggg catgcttggg atacgacctt
    15361 gcaagatatg ctgattcgtc aaaagagaat gcaaggatat gatactttat ggctgcctgg
    15421 tatggaccat gcaggaatcg cgacacaagc aaaagtagaa gcgaagctta gagagcaagg
    15481 gatttctcgt tatgatctag gtcgtgaaaa gttcatcgat caagtgtggg aatggaaaga
    15541 agagtatgcg agccacatcc gcgaacagtg ggctaaaatg ggcttatctt tagattacag
    15601 tagggaacgt tttacattag atgatggatt atctgaagca gtacgtaaag tttttgttac
    15661 cttatacgaa aaaggtttga tttatcgtgg tgaatatatc atcaactggg atcctcaagc
    15721 acgaacagct ctatctgata tcgaagttat tcataaagat atcgaaggcg ccttttatca
    15781 tatgagctat gaactagcag atggatcagg cgtagtagaa attgcgacaa cccgtccaga
    15841 aacgatgttg ggagatacgg cgattgctgt tcatccagaa gacgagcgtt atcaagcgct
    15901 catcggcaaa aaagtgattc tacctttagt caataaacaa atcccaatca ttgcagatga
    15961 atatgtagat atggaattcg ggacaggtgt agtaaaaatc actccagcac atgatccaaa
    16021 cgactttgag gtaggtaatc gtcatgatct tccacgcgta aatgttatgg atgatgatgg
    16081 tacgatgaat gaactagcag gaaaatatgc tggaatggaa cgttttgctg ctcgtaaagc
    16141 aatcgtgcaa gatctgaaag aaatcggtcg attgattaaa atcgatacga tgatccacag
    16201 tgtgggtcat tccgagcgga caggtgtcgt tgttgaacct cggctgtcta ctcaatggtt
    16261 tgtaaaaatg gcgccgttag cgaaaaaagc cattgataac caagaaacag atcaagcagt
    16321 ggaattcttc cctccaagat tcaatcaaac gtttttacgt tggatggaaa atgtccatga
    16381 ttgggtaatc tcacgacaat tatggtgggg acatcaaatt cctgcatggt atcacaaaga
    16441 aacaggtgaa atgtatgtag ggatggaagc accagcggat agtgaaaact gggtacaaga
    16501 tcctgatgtt ttagacactt ggtttagttc agctttgtgg cctttctcaa caatgggctg
    16561 gccagatacg gaaagcgaag attataagcg atacttccct actagcactt tagtaaccgg
    16621 ttatgacatc attttcttct gggtaagcag aatgattttc caaagcttgg aatttactgg
    16681 cgaacgtcca tttgagaatg tcttgattca tggattgatt cgtgacgaac aagggcggaa
    16741 gatgagtaaa tcattaggta atggaatcga tccgatggaa gtaatcgaaa agtatggagc
    16801 agatgctctc cgctggttct tatcgaatgg ttctgcacca ggacaagatg tccgcttcag
    16861 ctatgaaaaa atggatgctt cttggaactt tatcaacaaa atctggaatg cttcacgttt
    16921 cgttatcatg aatatcgaag gaatgaccgt agaagagatc gatttatctg gagaaaaatc
    16981 agtggctgac cggtggatcc ttactcgttt gaatgaaacg atcgaacgag taactgagct
    17041 atttgatcgt tttgaatttg gtgaagcagg tcgacaactg tataacttta tctgggatga
    17101 tttttgtgac tggtacatcg aaatgagtaa agaagtactt tatggagatg atgctgtttc
    17161 aaaacaaacc acccgtagca tcttagtcca tacacttgat cagatcctac gtttattgca
    17221 tccaatcatg ccattcgtta cagaagaaat atgggaacat attcctcatc agggaaattc
    17281 tctagtagtg gcagaatatc ctgttgtacg tccagagttt gatgatgaaa cagcttctaa
    17341 agggatggaa gtattgaaag aattgattcg agccgttcga aatattcgtt cagaagtgaa
    17401 tacaccattg tctaaaccaa ttacgttgct aattaaaaca aacgatgcta aaatcgatca
    17461 atttttagtt gaaaatacaa gctatatcga acgcttctgt aatcccgaag agttgacgat
    17521 atcaagtgag atcactgcgc cagatctagc gatgtcagct gttctgacag gtgcagaaat
    17581 cttcttgccg ttagcaggat tgatcaatat tgaagaagaa atcaaacgat tagaaaaaga
    17641 actggaaaag tggacaagcg aagtaaaacg tgtccaagga aaactttcta atgaacgttt
    17701 tgtttctaac gcgccggaag aagtagtaga ggcagagcgt gcaaaagaaa aagattattt
    17761 agagaagcaa acggctgtca aagaacgaat cgaaagtttg cgtactattc aataagaata
    17821 gaaaaagaga gtgtgaaatc agctagctat tactaaaagc tagctgattg aacatcgtta
    17881 caggaaagag tttgagcctc actcttagct tcgattcata aggagatgtg acataaattt
    17941 tgtcgcatct cttttttagt ctactcttat tatgtaggag aaggagggag gtggatcatg
    18001 tatcaagagt caataaagta gttgataaaa tcgttcctct ctctgtttct tttctctttg
    18061 tcatactttt atttttctta tgtgttctat ttgttataat aaaagagaaa tgggggaatt
    18121 gttgatgacg attgaagaag caatagaatg gatccatagt cgattaccat ttggttcacg
    18181 tcctggcctt gaccgagtcg aagctttatt agaagtggta gggcatccag agagaaaagt
    18241 aaaaacgatc catatcgctg gaacaaacgg aaaaggttct actgtagctt atttgagaag
    18301 cctattagaa gaggctgggc tgacagtagg tacatttact tcaccttata tcgaaacgtt
    18361 caatgaacgg atcgcaatca atggcaaaag cattccagac aatgatttga tccagcttgt
    18421 aaaaaaatat cagccactga tcaaacaact tgatcaattt gaagcagtag ctggtattac
    18481 tgaattcgag acattaacag caatggcatt tgattatttt ttgatgaaga aagtggacgt
    18541 agcgattatc gaagttggct taggcggctt attggattct accaatgtag ctgtaccaat
    18601 gctgacggga ataacaacga ttggacttga tcataccgaa atacttggag atacaattga
    18661 agaaatcgct gcacaaaaag ctggaatcat caaacaaggc gttccagtag taacaggaaa
    18721 tattgcccca gaagctttta ctgtcatcca acaaacagct aaagcaaaaa atgcaccgct
    18781 ttatgtatac ggggaaaatt ataaaataaa ctatctgcat cctgatgaga tgtggggaga
    18841 agtatttgaa ttttttaatg aaataggaaa gatccctcaa ttaaccactc cgcttttagg
    18901 gcaacatcaa gctgaaaatg caggagtagc catcgaattg tattttgttt actgtcaatt
    18961 ggaaaagctg cctttccgag aaaaagatgt tttgaaggga ttgaagaaat ctcgatggcc
    19021 aggaagaatg gagcgtttga gtaacgagcc attagttgtt ttagatggtg cccataatac
    19081 gcatgcaatg aaccggcttg ttgagaattt gaaaaaagaa ttcaaaggat atcaaatcaa
    19141 cattttgttt tccgccttac gaactaaaaa tgttagagaa atgctagaac gattgcagca
    19201 agtaccaaat gtccatattt atttgacgac ctttgaacat ccaaaagcca tcgaattaag
    19261 tgaagtatca gatttagcaa gtgagagagt atcaatcgtt tcattgtggc aattcggatt
    19321 agcagatata ttggaaaaga tgacaaatga agacgtatta gttgtgaccg gttcacttta
    19381 tttcatttca gaagttcgcc atttattaat acaattagga ggagatcatg aagtataatg
    19441 cagtgatttt tgacatggat ggtttgctgt tcgacacaga gttagtgtat tacgaagcca
    19501 cacaagtagt agcagacaag atgggttttc cttatgataa agacctgtat ttaaaattct
    19561 taggtgtttc agatgaagaa gtatgggcga attatcatat gattttctcg gaatttggca
    19621 aaacaagtgt acaaagattc atagatgatt cataccagga aacgcttgaa cgtttttcgc
    19681 aaggagcggt acaaatgaag cccggtgtgt tggaactcct ctctttttta gaagaacatc
    19741 agattcccaa agttgtcgct tccagtaacc aacgagcagt cattgaactt ttattgaaga
    19801 agaacaatct gtttgaacga tttgagacga ttgtttctgc agaaaatgtt caacgagcaa
    19861 aaccagaccc agaaattttc ttgttagcta atcagaagct aggtacagcg aagaaagaaa
    19921 cattgatttt agaagattca caaaatggta ttttagcagc ttctgcggct gagatcccgg
    19981 tagtcatggt accagatctg cttccaccat cccaagaatt gatcaaaaaa acagctgggg
    20041 ttgtttcttc tttacatgaa atccctgcat tgttaaaata aaaatccatc tcttttcgta
    20101 tcttatttta gataagatag aaaggagatg tttttttggg aaaacgattg atcaaagaag
    20161 tacctactag ctctttacca agggaacgta tggaaattta tggcgcggaa gctttatcgg
    20221 atcaggagct tttggcaatc ttactgcgta ccggtcaaca cccttatagt gtgatgtcga
    20281 ttgctggaaa tctgttaaaa acgtttggag gactggcctc tttacgacag gctactttgc
    20341 atgagttaga agagattcga gggataggac gcgtgaaagc aatcgaaatc aaagccttga
    20401 tcgaattagg tcgacgaatt cagacggatc atgaaagagc ggctccaccg gttcgtacca
    20461 gctacgaatt ggcccagcaa ttgattctgg aaatgaaaga tcacaaacaa gaacatttgg
    20521 tttgtattta tttggacacg aagaatcaag tgatcttaaa aaaaacagtg ttcatcggtt
    20581 cgctgaatca gtcagtcgct catcctagag aaattttcca ttatgctgta cgttattgtg
    20641 cagcaaggat cgttttagcg cataatcatc cttctggtaa tgttattcca tcgcagcagg
    20701 atatgaattt taccaaaagg atacaaaagt gtggagaaat gatggggatc actgtactgg
    20761 accacttgat catcggacgc aaacgatatt ttagtttgag agaagaagga atgatggaag
    20821 agaagtaagt tgctttcttg caaaaaaaat gaagcaagtg tacaattata ttgtaatttt
    20881 gtttgggaat agaagttgaa acatctgttg cacctctatc gtacacgagc aatgttacaa
    20941 tttaaattag tgcttttggc acgaggagga tattattatg gaaaacggaa cagtaaaatg
    21001 gttcaacgct gaaaaaggat ttggatttat ctcacgtgaa gatggaagcg acgtattcgt
    21061 tcacttctca gctatccaag gtgacggatt caaaacatta gaagaaggtc aagcagtgac
    21121 tttcgacgtt gaagaatcag accgcggccc acaagctgca aacgttgtaa aagcttaatt
    21181 ttatctgtaa atcaaattga tggtagacat caatttttac gagagaacaa agcgagtaac
    21241 aaa
//
LOCUS       DASHHB010000047        20940 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.049, whole genome shotgun sequence.
ACCESSION   DASHHB010000047 DASHHB010000000
VERSION     DASHHB010000047.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20940)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20940)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20940
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.049"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            166..360
                     /locus_tag="U2K71_001995"
     CDS             166..360
                     /locus_tag="U2K71_001995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316044.1"
                     /translation="MYYVEVKTKGVKNKQYVKGISNEYPLLGSWKEAAPFSKPCAIKI
                     KNELEKELTCGKAVVDIIEK"
     gene            629..928
                     /locus_tag="U2K71_001996"
     CDS             629..928
                     /locus_tag="U2K71_001996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316045.1"
                     /translation="MDYTVEFDSELLKQFPQEEHFMYKEAISHKVTEYLEHNFFRIKP
                     VRTRSAKGIYEMKVKINKKNYRIAFALKKNHVFIFYISSNLQKIVFDKEVSKKIK"
     gene            complement(1025..1459)
                     /locus_tag="U2K71_001997"
     CDS             complement(1025..1459)
                     /locus_tag="U2K71_001997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7316046.1"
                     /translation="MNIDSLMIDEISELYKSVGWTNYTKDTARLARAFEQSESLIKRN
                     GEGKIIGIVRWITDFATIAFIQDILIHPRYQRQGIGKVLLNEALEKITSYGPVQIELL
                     TDDTEKTKKFYESVGFVQVKEMGGISYVIDTRRKKVLFGNDK"
     gene            complement(1691..2401)
                     /locus_tag="U2K71_001998"
     CDS             complement(1691..2401)
                     /locus_tag="U2K71_001998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 4-epimerase"
                     /protein_id="HEM7316047.1"
                     /translation="MSCEEKIAAMKQRVLEANLALPRYGLVKLTWGNVSEVDRELGVI
                     VIKPSGVRYECMQADQMVVTDLSGNIIEEDSLKPSSDLPTHVVLYQTFEDITAITHTH
                     STHSVMWAQAGRDLPAYGTTHADAFYGKVPCTRQLTKEEVREAYEVHTGNVIVETFKE
                     RKLDPNEVPGVLVYGHGPFTWGDSPMKAVENSLILDEICLMAKENELINPNICEIPQY
                     LLDKHYFRKHGKYAYYGQ"
     gene            complement(2391..3251)
                     /locus_tag="U2K71_001999"
     CDS             complement(2391..3251)
                     /locus_tag="U2K71_001999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ribulose-5-phosphate 3-epimerase"
                     /protein_id="HEM7316048.1"
                     /translation="MAQIGIYEKALPKDISWKERFSLVKEMDFDFIEMSIDETDERLA
                     RLDWSEEKIAELREEMFSSGVRIHSICLSAHRRFPFGSADPEKKKAAKQLMKKAIHLA
                     HNLSVKVIQIAGYDVYYEEKSMNSREDFIEGIKESVKEASKYGIILSVEIMDDPFMNS
                     ISKFLEIKEQIHSPFLQVYPDLGNLSAWPENNPARELEKGIDYITAIHLKDTYPVTKE
                     YSGQFRDVPFGSGCVDFLGLLRHLKRLDYDGTFLIEMWSEKSQDFRREIQQAKDYLSS
                     KLKEAGYDVL"
     gene            complement(3256..3894)
                     /locus_tag="U2K71_002000"
     CDS             complement(3256..3894)
                     /locus_tag="U2K71_002000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-L-gulonate-6-phosphate decarboxylase
                     UlaD"
                     /protein_id="HEM7316049.1"
                     /translation="MSRPNLQIALDHNDLEHALGDVMKVGDVVDIIEVGTILCLQEGQ
                     KAIRCIRSMFPDKKLVADTKCADAGGTVASNVAKAGADWMTVICCATIPTMEAAQKEI
                     GELQVELYGNWTFEQAMDWHNIGIRQVIYHQSRDALLSGETWGEKDLSKIKKLIELGF
                     NVSVTGGLNPHTLHLFEGIDVYTFITGRGITAANDPMKAAQNFKDEIIRIWG"
     gene            complement(3909..4208)
                     /locus_tag="U2K71_002001"
     CDS             complement(3909..4208)
                     /locus_tag="U2K71_002001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7316050.1"
                     /translation="MRILVSCANGSGTSLMMMRSVEKAMKELNVPITKIHHCAISEGK
                     SSAGQYDVVFTPVNFLNMFTQAEKKGVTVIGIKNVMSVKEISEKFAETKWYQKNK"
     gene            complement(4251..5735)
                     /locus_tag="U2K71_002002"
     CDS             complement(4251..5735)
                     /locus_tag="U2K71_002002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS ascorbate transporter subunit IIC"
                     /protein_id="HEM7316051.1"
                     /translation="MGEFLLSIWTYFATNILTQPAYLIGFIVLLGYILLKRPLHECIA
                     GFLKATVGYFILTVGSGGLVNNFRPILVGLKERFNLDAMVTDPYFGQNAVDAGLMETF
                     GRTFGDVMILLLIAFVLNILLVRFQKYTKLRSVFTTGNVQVQQAATAFWILLFCFPDL
                     GRIQALIFMGLILGCYWTVASNLTVGITQELTEGAGFAVAHQQMFGIFIFAKLAEWMK
                     KRDEKKNRSIKQDKKIEDIKLPGFLSIFNENMVATSLLMLFFFGIILIVLGKDYLIQA
                     QFMQEGQSFLFYIMTTSLNFAVYLAILQLGVRTFVDELTQSFQGISNTILPGAVPGID
                     VAATFGFGSPNAVTIGFLFGALGQFLMIGLLILLKSPTIVIAGFIPLFFDNAVIAVYA
                     NNRGGFKAACLFPFLSGIIQVGGSALFATWIGMSTYGGYIGMFDWATAWPLFTVIMKV
                     LGYAGIAVVVIGLLAIPQLQYRKNPDGYFLIVEDYDTYAEKQRV"
     gene            complement(5755..6216)
                     /locus_tag="U2K71_002003"
     CDS             complement(5755..6216)
                     /locus_tag="U2K71_002003"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM7316052.1"
                     /translation="MLKYFFDHQLIHYPDKEITDWREAIAESCLVLIDKEIIDETYVE
                     EIIHCVEKYGPYIVIAPQVAMPHSSEKSDGVFGTAISFTKLKQPIRFESEGENEEKTA
                     SLFFTLAAKDPEEHLENIQKLSELLMTDGLVERLISTNSIDDYQVVMAEYN"
     gene            complement(6234..7301)
                     /gene="ulaG"
                     /locus_tag="U2K71_002004"
     CDS             complement(6234..7301)
                     /gene="ulaG"
                     /locus_tag="U2K71_002004"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-ascorbate 6-phosphate lactonase"
                     /protein_id="HEM7316053.1"
                     /translation="MSKTVNEVTKEKWLVETFPEWGTYLNEEIETEVVDPGTFAMWWL
                     GCTGIWLKSHEGTNILCDLWCGTGKRSHGNGMMKKGHQMMRMSGVQNMQPNLRTQPFV
                     IDPFAIKNVDALIVTHIHSDHLDIHTAAAVHQNCPEATFIGPKEVVRTWQKWGIPEEK
                     TITVKPGDAVKIKDINVVALEAFDRTALVTCEDPEEVLKGKMPQEMDQIAVNYLFETS
                     GGNLYHAGDSHYSNLFAKHGNEHKIDVCLGAYGENPRGITDKVTSVDMLRMAESLNAK
                     VVIPVHYDIWSNFMADPKEITEIWKFKKDRLKYGFKPYIWQVGGKFIYPTNKDDLEFN
                     FYRGFEDVFSIENDTPFPSFL"
     gene            7519..8289
                     /locus_tag="U2K71_002005"
     CDS             7519..8289
                     /locus_tag="U2K71_002005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM7316054.1"
                     /translation="MKNSLVNIEKRQKEILELLQQTEQLSTFELAESLDVSISTIRRD
                     LNLLEEKNEIIRNYGYCSYNYKNQTDFDQSGPERIKQAIAREASKYINDYDTLFINSS
                     STALKTLDFLQSNHLTVITNNVKINYSPHKDNCSYILTGGEMRFPKEALVGDIALNTI
                     SSVNADICIIGCSGVDVVHGVTTKILNESTINEMMVNKTIKCKILVADHRKIGLTSKF
                     KIADISVFDYLITDKFCSEKVLEEIRNTGIKTIQVDSF"
     gene            complement(8562..9389)
                     /locus_tag="U2K71_002006"
     CDS             complement(8562..9389)
                     /locus_tag="U2K71_002006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7316055.1"
                     /translation="MIKVDGALLKEYRKKKGLTQVQLAEGICEQATVSNIENKNMSPG
                     INILSALCKKLNVEISDILVETEEDKIRKKINEIQSLNSVSKYQESYKILQTIDQEKV
                     SNSDLLNQILFYQGLLDHLYAKKPDAALFYFNQVLEQTTETDIHHLQAAANVAMIYLS
                     KGELDFAKVYVERTLKILSETDFDNLMVCIVYYDIATYYRKIEDYDKAIQLCEKGIEY
                     NKKHKSTYALEYLLYEIASCHKQLGEDDYLERYMDAKKIARFNGNDYAVKVIENDLK"
     gene            9957..11192
                     /locus_tag="U2K71_002007"
     CDS             9957..11192
                     /locus_tag="U2K71_002007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase"
                     /protein_id="HEM7316056.1"
                     /translation="MKTILFAPAVFNLAETTRMIEVAKAMKDRYYCVFFGFSKTFAPL
                     ITESGFEYHLLAPILTKKQEEQIIKFDQLRTLKNPFTKEMVASRITSEQSLIAKFEPR
                     LIVTGSNVTIFLSARNKKIPLVYVKPYALSEPFFQSEHALMPHELRFFGPLSNPLWHW
                     TKKRIVNTRWIPNAFKHAAQKEMLPLPKTSLQMMDADLNLITTPKLFLEEEQLPENYR
                     IVGPIFAKLSTSLPEEVLTFIQRKQQERKRIIYFAMGSSGNPRMLRKILFFLQKQPDL
                     AVIAPIDYLLDDIQSDSEDLLITEYLSSHLLHEFIDFSFIHGGEGTVQTACESGKPFI
                     GVGMQFEQSCNIDYCVKYGNAIALTKPVTERKLQIALREVSSPKIRQQARTLQAVFPI
                     NGPQQAIQEIETFLSNHPI"
     gene            complement(12191..12451)
                     /locus_tag="U2K71_002008"
     CDS             complement(12191..12451)
                     /locus_tag="U2K71_002008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase"
                     /protein_id="HEM7316057.1"
                     /translation="MYWLILFFVFIFLLTASHLILNMLATYHIQINRWIWALASFLIV
                     ILPKIIVPHMNVLFSWGTYVLCGIFAINFMIEQHRWFVTSKL"
     gene            complement(12478..13779)
                     /locus_tag="U2K71_002009"
     CDS             complement(12478..13779)
                     /locus_tag="U2K71_002009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7316058.1"
                     /translation="MEKLMKWVEEKLVPPMAKIGTQRHLLAIRNGVVSTLSLILIGTF
                     FMVFINLPFPGWNEFIAPYSATIVLPFRMTMGLMAIYATFVMGSDLAKSYGLDSVTGG
                     ILSLGTFFMLQVPVNVLTPEEAPLGWVLPMSSLGASGMFAGILSMIFAVEVYRFFKNR
                     NITIKMPEQVPPAVARSFEALIPGAVILTITWLIRSVAGFDVNAALLSLFEPLTNILG
                     NNLLGVLLPMFLIHLLWSFGIHGMSIVGAVVRPMWLIMLDENAKALADGTAATQLPYI
                     APEQFYQWTVTIGGAGATIVVSVLFIFFCKSKFLKEVGRFSIIPGIFNINEPIIFGAP
                     MMLNPYMFIPFNLVPLVLTIISYFAVKLEMVNRFTVLPTWTLPAPIGGYLSAGNDWRV
                     VILIMINTLIAFIIYYPFVKAYDKKMLSDEQSRGENEVQTV"
     gene            complement(14071..14529)
                     /locus_tag="U2K71_002010"
     CDS             complement(14071..14529)
                     /locus_tag="U2K71_002010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM7316059.1"
                     /translation="MHFFKTRNILKAVASGQIIPLTAVKDEVFSSKMIGVGYAITNHD
                     GYIHAPVKGIIDNIFPTRHAITIESESGRKILIHMGLDTVELEGSPFQIRVESNQEVK
                     EGTLLAIIDLKKLEQAQKDTVIIVVSPGIFEGNLLKQNQEVKIKDSCFEF"
     gene            complement(14542..15873)
                     /locus_tag="U2K71_002011"
     CDS             complement(14542..15873)
                     /locus_tag="U2K71_002011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="maltose-6'-phosphate glucosidase"
                     /protein_id="HEM7316060.1"
                     /translation="MMMKKQIITIAGGGSTYTPGIVQAILNNEKRLPLSEIRLYDIDE
                     ERNMDMYLIVKFMLKRKGFSNIRIRATDDPKLAFTGCDFVFSQIRVGGLEMREKDEKI
                     PLKHGLVGQETCGLGGFAYGMRSIKGLLEIVDHIQDYAPKAWILNYTNPESIVSEAVR
                     RKYPNVKMINACDMTISIEETIAVNYGYDRKNWIVTYYGLNHFGWYTSIYDKELQREI
                     MPEIIEKLITKEMQVADFNIGDKTWQKTFQMMSVITKNFPSNIPNNYLEYYLYPDMVV
                     EHTDKEYTRANMVMDGREKNTKEMADKIRRGIVEEVLNFNFGEHGQYIVDIAISLLND
                     DRRRFMLIVPNQGAIPNLRSDAVVEIPAYVGATGVEPITLRKPISDFHKGLMEAQVAV
                     EKLLVDAYFEGSYQKALQAFTLNQTVPNARVAKKILDEMIKVNKKYWPALK"
     gene            complement(15884..17326)
                     /locus_tag="U2K71_002012"
     CDS             complement(15884..17326)
                     /locus_tag="U2K71_002012"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM7316061.1"
                     /translation="MRIFNRGGIKMNVKSNVQKLGRAMLLPVAAMPLAGLIMRLSADD
                     MLNIPVIGAAGNAVFGNLDILFAIGVAIGFVKNKDKGIPALTGFLAIATLKKGLEIMD
                     PEVNMGIFGGIISGLVAAWTYNRFKEQKLPVVFSYFAGEKFPLTMVMILQTIAAVFLG
                     FIWPLIQIGIDNFAKLLVDMGALGVGIFMFLNRLLIPFGLHHVLNTYIYYDLGSYTSP
                     SKEVFRGEMTRFINGDPQAGLFLSVFFVVMMFGIPAICLAIYKAAYKENKEMVKGIMG
                     SGAGTSFIANITEPVEFSFMFLSPMLYVIHALFAGLAGIVCYLFNIRIGFTFGACIVD
                     YLINFRIATNAILILPIGIFFFALYYVTFYYLINKRNIQTLGREAKAEFGNEVTLEET
                     ELGLASKNYEYMATKMLQAFGGKANILDVYSCNTRLRVEVVDPTMVEEQRIKQLGISG
                     IIKPTEKNYQIIIGLEVTYVMAEFNKLLEE"
     gene            complement(17313..18077)
                     /locus_tag="U2K71_002013"
     CDS             complement(17313..18077)
                     /locus_tag="U2K71_002013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318971.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate deacetylase"
                     /protein_id="HEM7316062.1"
                     /translation="MGKVIFNSDDFGYSHGVNYGIMDAYQRGILTSTTLMANMPGFEH
                     AVKLRKEMPRLGVGVHLTLTCGKPLLKTVDTLIESGEFRSLSYYQHPFNIDKDQLYQE
                     WNAQIQKIYHAGIIPTHLDSHHHTHTLDENQEVVVTLAKKYDLPVRSNFERKDEVRHV
                     DYFEPCFDVVGETDEKKRQINTSVEEYLASLLMILREDKTIEVMCHAAYLDQELLNGS
                     SFVYPRTHQVEFLIHSAFAQKVKNDPLIELVTYEDI"
     gene            complement(18181..19056)
                     /locus_tag="U2K71_002014"
     CDS             complement(18181..19056)
                     /locus_tag="U2K71_002014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM7316063.1"
                     /translation="MKESASCFVLSYRGGELTVYVMKKLNHNAVLIDDDGIEKIAMGK
                     GIGFSAKVSKPFDVEDADKLFVLDSKEKIKRFSEIVSQIPIEFMDFAEEVIELITARI
                     SSPLDSNIYITLTDHIYFAIQRKREDRDVTAIMLPEMRFLYPNEYDIAVEVVERINHR
                     YQTELGSNEVGFITMHIINAELGEPNSLKSLKIVEMTTTILSHLEKSYSNVYDKESLT
                     YHRLMVHIKFLVKRLIYQEETPVENIGFFTETFKHSKAYCTALKIKDIIEQQFQLAIQ
                     ENEIIYLAIHLARIY"
     gene            complement(19311..19395)
                     /locus_tag="U2K71_002015"
     tRNA            complement(19311..19395)
                     /locus_tag="U2K71_002015"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19359..19361),aa:Leu,seq:caa)
     gene            complement(19413..19484)
                     /locus_tag="U2K71_002016"
     tRNA            complement(19413..19484)
                     /locus_tag="U2K71_002016"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19450..19452),aa:Gln,seq:ttg)
     gene            complement(19494..19566)
                     /locus_tag="U2K71_002017"
     tRNA            complement(19494..19566)
                     /locus_tag="U2K71_002017"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19531..19533),aa:His,seq:gtg)
     gene            complement(19628..19712)
                     /locus_tag="U2K71_002018"
     tRNA            complement(19628..19712)
                     /locus_tag="U2K71_002018"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19676..19678),aa:Leu,seq:caa)
     gene            complement(19733..19803)
                     /locus_tag="U2K71_002019"
     tRNA            complement(19733..19803)
                     /locus_tag="U2K71_002019"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19769..19771),aa:Cys,seq:gca)
     gene            complement(19849..19920)
                     /locus_tag="U2K71_002020"
     tRNA            complement(19849..19920)
                     /locus_tag="U2K71_002020"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19886..19888),aa:Gln,seq:ttg)
     gene            complement(19932..20004)
                     /locus_tag="U2K71_002021"
     tRNA            complement(19932..20004)
                     /locus_tag="U2K71_002021"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(19969..19971),aa:His,seq:gtg)
     gene            complement(20007..20080)
                     /locus_tag="U2K71_002022"
     tRNA            complement(20007..20080)
                     /locus_tag="U2K71_002022"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20046..20048),aa:Trp,seq:cca)
     gene            complement(20087..20167)
                     /locus_tag="U2K71_002023"
     tRNA            complement(20087..20167)
                     /locus_tag="U2K71_002023"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20131..20133),aa:Tyr,seq:gta)
     gene            complement(20169..20244)
                     /locus_tag="U2K71_002024"
     tRNA            complement(20169..20244)
                     /locus_tag="U2K71_002024"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20209..20211),aa:Phe,seq:gaa)
     gene            complement(20280..20352)
                     /locus_tag="U2K71_002025"
     tRNA            complement(20280..20352)
                     /locus_tag="U2K71_002025"
                     /product="tRNA-Asp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20317..20319),aa:Asp,seq:gtc)
     gene            complement(20362..20434)
                     /locus_tag="U2K71_002026"
     tRNA            complement(20362..20434)
                     /locus_tag="U2K71_002026"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20399..20401),aa:Val,seq:tac)
     gene            complement(20450..20521)
                     /locus_tag="U2K71_002027"
     tRNA            complement(20450..20521)
                     /locus_tag="U2K71_002027"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20486..20488),aa:Glu,seq:ttc)
     gene            complement(20536..20625)
                     /locus_tag="U2K71_002028"
     tRNA            complement(20536..20625)
                     /locus_tag="U2K71_002028"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20589..20591),aa:Ser,seq:gga)
     gene            complement(20665..20738)
                     /locus_tag="U2K71_002029"
     tRNA            complement(20665..20738)
                     /locus_tag="U2K71_002029"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(20702..20704),aa:Asn,seq:gtt)
     gene            complement(20748..20863)
                     /gene="rrf"
                     /locus_tag="U2K71_002030"
     rRNA            complement(20748..20863)
                     /gene="rrf"
                     /locus_tag="U2K71_002030"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT         6602 a         4390 c         3331 g         6617 t
ORIGIN      
        1 agaacaatag ccttttatta tatatattat tcaaattagt tatatcgagt gaaaaaaact
       61 ttcaaattga ttctttttat ttatcataaa gatatagata cataatcatt ttcacttaca
      121 tatatttgaa tattaactaa aaacagcaag aaagaagagg atcaaatgta ttatgtagaa
      181 gtaaaaacaa aaggcgtcaa aaacaaacaa tatgtgaaag gtatcagtaa cgaatatcct
      241 ttacttggtt catggaaaga agctgctcct ttttccaaac catgtgccat aaaaataaag
      301 aacgagctag aaaaagaact gacttgtggt aaagctgttg tagatatcat cgaaaagtaa
      361 atccaatgta tagaaaggaa gatcacacat gaaaagcgac gtacaaattg ctaagattat
      421 agaattagaa gaagaagacg cagtgttgac aattgaaaaa aaataaatgt ttgtatacca
      481 tatgattatt tcaatatgta tcctggaatt ggagatctcg tagagctata tcaagatgaa
      541 tcaaatatac ttgtcattca tcatgaaata tagagactta taaaattgtt tatttttata
      601 atgtatcagt atagaaagga aataaaacat ggattatact gttgaatttg acagtgaact
      661 acttaagcaa tttccacaag aagaacattt catgtataaa gaagcaattt cccacaaagt
      721 gacagagtat ttggagcata atttttttcg tataaagcca gtgcgtactc gttcggcaaa
      781 aggaatatac gagatgaaag tcaaaattaa taaaaaaaat tatcgcatag catttgcttt
      841 aaaaaaaaat catgtgttca ttttttatat tagttcgaat ttgcaaaaaa ttgtatttga
      901 taaagaggtg agtaaaaaaa taaaatagct gagcattgga ggatagattg agtgatacta
      961 agtctatcct cttgtttttc taaagaacta agctagaatt ttttcttttg tacgttatta
     1021 tatgttattt gtcattacca aaaagcacct tttttcttct agtatctatg acataagaaa
     1081 taccacccat ttcctttact tgaacaaatc ctactgattc ataaaatttt tttgttttct
     1141 cagtatcatc tgtcaataat tcgatttgga ctggtccata actagtgatc ttctccaaag
     1201 cttcattcaa aagcactttg cctattcctt ggcgctgata cctcggatga atgaggatat
     1261 cttgaataaa tgcgatcgtc gcaaaatcag tgatccaacg aacgattccg ataatcttcc
     1321 cttccccatt acgctttatc agagattcag attgttcaaa tgctctagct aaccttgcag
     1381 tgtcttttgt gtaattcgtc caaccaacgc tcttatataa ttcagaaatc tcatcgatca
     1441 taagggaatc aatattcatt tttttcttcc tttcttatac aattctccct ctaataatac
     1501 agttctttga ctatttaatc tatcacatat aactctcctg taaagattct ctcgattcct
     1561 taaaattcct ctttcctttt agaatgcata ctttttgata aataaaaagg ttgtgataaa
     1621 ttttcttcac aacctctgtt tctctctatc aagtgttatc ttttaatttt ctacccttac
     1681 cctagcatct ctattgcccg tagtatgcat actttccatg tttgcgaaaa taatgtttat
     1741 ctagtaaata ttgcgggatt tcacatatat tggggttgat cagctcgttt tctttggcca
     1801 tcagacaaat ttcatctaag atcaaactgt tttctactgc tttcataggt gaatcccccc
     1861 atgtgaacgg tccatgtcca tagacaagta ctccagggac ttcgtttgga tctaattttc
     1921 tttccttaaa cgtctcgaca atcacattcc ctgtatgaac ttcgtaagct tctcttactt
     1981 cttctttcgt taattggcgt gtacaaggaa ccttaccata aaaagcatca gcatgagtcg
     2041 tgccataagc tggcaaatcg cgtcctgctt gcgcccacat gacggagtgc gtggaatgag
     2101 tatgagtaat agcagttata tcttcaaaag tttgatacaa aacaacatgt gtggggagat
     2161 ctgaagatgg ttttaagcta tcttcttcta ttatatttcc cgacaaatct gttaccacca
     2221 tttgatctgc ttgcatgcat tcatatctga caccgcttgg cttaatgacg atgactccta
     2281 gctcacggtc cacttcactc acattgcccc aagtcaattt gactaatccg tatcgcggca
     2341 atgccagatt tgcttcaagg acacgttgct tcattgctgc aattttttct tcacaagaca
     2401 tcatagccag cctccttcaa tttagaactt aagtagtctt ttgcctgctg gatttctctt
     2461 cgaaagtctt ggcttttttc actccacatt tcaatcagaa atgttccatc ataatctaga
     2521 cgtttcaaat gtcttaataa tccaagaaaa tcaacacaac cgctaccaaa aggaacatca
     2581 cgaaactggc ctgaatattc ttttgttaca gggtaagtat cttttaagtg gatagcagtg
     2641 atataatcga tccctttctc aagttctcga gccggattat tttccggcca tgcggacaaa
     2701 ttgcctaggt ctggatatac ttgtagaaaa ggtgaatgaa tctgttcttt gatttctaaa
     2761 aattttgaaa tggagttcat aaatggatca tccatgattt ccacggaaag aatgatgcca
     2821 tatttagaag cttcctttac gctttctttt attccttcga taaagtcttc tctggaattc
     2881 attgatttct cttcataata aacatcgtat ccagctattt ggattacttt gacgcttaga
     2941 ttatgcgcca aatggattgc ttttttcatt agttgtttcg ctgctttttt cttttctgga
     3001 tcagcagaac caaacggaaa tcgccgatgc gcacttaaac aaatcgaatg gatacgtaca
     3061 ccggagctga acatctcttc tctcagctca gctatctttt cttcagacca atctaatcgt
     3121 gctaaccgct catccgtctc atcaatcgac atctcgataa aatcgaagtc catttctttt
     3181 acaagggaaa aacgttcttt ccatgaaata tctttgggta acgctttttc ataaatccca
     3241 atttgagcca tttccttacc cccagatacg aatgatctca tctttgaagt tttgtgcggc
     3301 cttcatcgga tcatttgctg ctgtaatacc acgaccagtg ataaaggtat acacatcgat
     3361 cccttcaaat aagtgtaatg tgtgtggatt taatccacca gttacagaaa cattaaagcc
     3421 tagttcaatc agttttttga tcttcgataa atctttttca ccccaagttt caccagataa
     3481 cagcgcatca cggctttgat ggtagattac ctgccggata ccgatgttat gccaatccat
     3541 ggcttgttca aaagtccaat tgccgtagag ttctacttgc aattcaccta tttctttttg
     3601 tgctgcctcc atagttggaa tcgttgcaca acaaatgact gtcatccagt ctgctcctgc
     3661 ttttgcaaca ttactcgcca ctgttcctcc agcgtctgca catttcgtat cagcgactag
     3721 ttttttatct gggaacatac tgcgaataca gcgaatcgct ttttgtcctt cctgtaagca
     3781 aagaattgtg cccacttcaa taatatctac tacatcacca actttcataa catctcctaa
     3841 tgcatgttct aagtcattgt gatctaacgc aatttgcaaa ttcggcctgc tcatcttagt
     3901 tccttccttt acttgttttt ttggtaccac tttgtttcag caaatttttc actgatttct
     3961 ttgactgaca tcacattttt gataccgata actgtaactc cttttttctc tgcctgagta
     4021 aacatattca aaaaattgac tggcgtaaaa actacatcgt attggccagc tgaacttttt
     4081 ccctctgaaa tggcacaatg atggattttg gtaattggta cgtttagttc tttcattgct
     4141 ttttccacac ttctcatcat catcaagctt gttcctgatc catttgcaca cgatactaaa
     4201 attctcatct taatccctcc attatgagtg ttgtctaatc agattttctt ttatactctc
     4261 tgcttttctg catatgtgtc atagtcttct acaatcaaaa aataaccatc aggatttttc
     4321 cgatattgta actgcggaat agccaataaa ccaatgacaa ctacggcaat tccagcataa
     4381 cctaggactt tcatgattac tgtaaacaat ggccaagctg ttgcccagtc gaacatcccg
     4441 atataaccac cgtatgttga catcccaatc catgtagcga ataatgctga accgcctact
     4501 tgtatgattc ctgaaagaaa cgggaacagg caagcggctt tgaatccacc tcggttattg
     4561 gcataaactg ctataacggc attatcaaag aacaacggaa tgaaaccggc tatgacgatc
     4621 gttggtgatt tcaataaaat cagtaaacca atcatcaaaa actgtcccaa agcaccaaac
     4681 aagaacccaa tcgttaccgc atttggcgaa ccaaagccaa aagtagcagc tacgtcaatt
     4741 cctggaacag ctcctggtaa aatcgtgttt gagattcctt ggaaagattg agttagttca
     4801 tcgacgaatg tccgaacacc taattgcaag atcgccagat ataccgcaaa atttaaagaa
     4861 gtcgtcataa tgtagaataa gaaactctgc ccttcttgca tgaattgcgc ttgaatcaaa
     4921 taatctttgc ctagtacaat cagaatgatc ccaaagaaaa atagcatgag taacgaagta
     4981 gcaaccatat tctcattgaa aatcgacaag aatcctggta atttgatatc ttcaattttt
     5041 ttgtcttgct tgattgatct atttttcttt tcatcccgct ttttcatcca ctcagctaat
     5101 tttgcaaata taaagattcc gaacatttgc tgatgtgcca ctgcaaatcc ggcaccttct
     5161 gtcaattcct gcgtgattcc tactgttaag ttagaagcta ctgtccaata acaacctaaa
     5221 atcagtccca taaaaatcaa tgcttgtatc ctacctagat caggaaagca aaacagtaaa
     5281 atccaaaaag cggtagctgc ttgttgaacc tgaacgtttc cggtagtgaa aacggaacgc
     5341 agtttcgtat atttctgaaa acggactaat aagatattta aaacaaatgc aatcagtaat
     5401 aaaatcatca catctccaaa ggtacggcca aatgtctcca ttaatccagc atccacagca
     5461 ttttgtccaa aataaggatc tgtaaccatg gcatccaaat tgaaccgttc ctttagtcca
     5521 accaagattg gccgaaaatt attgaccaaa ccaccagagc caacagtcaa aataaagtaa
     5581 ccgaccgttg cctttaagaa tccagcgatg cattcatgaa gcgggcgttt cagcaaaata
     5641 tagcctaata acacgataaa accaatcagg tatgctggct gcgtcaagat atttgttgca
     5701 aaatacgtcc aaatgcttaa caaaaattcc cccatctttt ttctcctcta tcaattaatt
     5761 atattctgcc atcacaactt ggtaatcatc gatggagttc gtagagatga gacgctcaac
     5821 caatccatct gtcattaata attcggataa tttttggata ttttctagat gttcttctgg
     5881 atcttttgct gcgagtgtaa aaaacaaact agccgttttc tcctcattct ccccttcact
     5941 ttcaaaacgg attggctgtt tcaacttggt aaatgaaata gctgttccaa aaacaccatc
     6001 acttttttct gaagaatgcg gcattgctac ttgaggtgcg atcacaatat aaggaccgta
     6061 tttttctaca caatggatga tctcctccac atacgtttca tcaataattt ccttatcgat
     6121 caatacaaga cagctttcgg cgatagcttc acgccaatcc gtgatttctt tatctgggta
     6181 atgaatcaat tgatggtcaa aaaagtattt cagcaagaaa ctccccttct ttcttataaa
     6241 aacgacggaa acggtgtatc attttcaatc gagaagacat cttcaaatcc tcgataaaaa
     6301 ttaaactcaa gatcatcttt gtttgtcgga tagatgaatt tcccgcctac ttgccagata
     6361 taaggtttaa atccatactt caggcgatcc tttttgaatt tccaaatttc agtgatctct
     6421 tttggatcag ccataaaatt agaccaaata tcgtagtgga caggaatcac tacttttgca
     6481 ttcaatgact ccgccatgcg cagcatatca acagaagtaa ctttgtctgt tatccctcgt
     6541 gggttttcac cataggcccc taaacagaca tcgattttgt gttcattacc atgtttggca
     6601 aataaattgg agtaatgtga atctcctgca tgataaagat tccctcctga tgtttcgaat
     6661 aaatagttta ctgcaatttg atccatctcc tgaggcattt ttccttttaa tacctcttct
     6721 ggatcttcac aagtgaccaa cgctgtccga tcaaacgctt ctaaagctac tacattgatg
     6781 tctttgattt tgactgcatc acctggcttt actgtaatcg ttttttcttc aggaattccc
     6841 catttttgcc acgtgcgaac cacttctttt ggcccaatga atgttgcttc aggacaattt
     6901 tgatgaactg ctgcggctgt atggatatct agatgatcgg aatggatatg tgtaacgatt
     6961 aaagcgtcta cattttttat cgcaaaagga tcgattacga aaggctgagt acgtaaatta
     7021 ggctgcatat tttgaacgcc actcatacgc atcatctgat ggcctttttt catcatgcca
     7081 ttcccatgac tacgttttcc cgtcccacac catagatcac ataaaatatt cgttccttca
     7141 tgacttttca gccaaattcc cgtacagccc agccaccaca tagcaaaagt tccaggatcc
     7201 actacttctg tttcgatttc ttcattcaag tatgtgcccc actcaggaaa tgtttctact
     7261 aaccactttt cttttgttac ttcattgaca gttttactca tttgcttgct ccccccatat
     7321 cgttgttcca acttacaagt aaatattaat gtgtatcagc atccatgaaa acccttttat
     7381 atgaccgttt gttttttata tgattttctt tcagtaatca atgattgttt cagtataata
     7441 aagacagata tgaatgttta aatgaacaac tagtcaaata aatccatgga tcaagatgtc
     7501 cctcaggagg caaaagtcat gaaaaactca cttgtcaata ttgaaaaacg tcaaaaagaa
     7561 attttggaac tgcttcagca gactgaacaa ctatcaactt tcgaattagc tgaatccctt
     7621 gatgtatcta ttagtacgat acgaagagat ttaaatttat tagaagaaaa aaacgagatc
     7681 atacgcaatt acggttattg ttcttataat tacaaaaacc agactgattt tgatcaatct
     7741 ggtcctgaaa ggattaaaca agcgattgcc agagaagcta gtaaatacat taacgattat
     7801 gatacgctgt ttatcaattc tagctcgact gcgttgaaaa cactggattt tcttcaatct
     7861 aaccatttga cagtcattac aaacaacgtg aaaatcaatt actctccgca caaagataat
     7921 tgtagttata ttttaactgg tggagagatg cgctttccaa aagaagcact agtgggcgac
     7981 atcgcattaa atacgatttc ttcagtcaat gcagacattt gtatcattgg ctgtagcgga
     8041 gtcgatgtag tacatggggt cacaacaaag atattaaatg aatcaacgat caacgaaatg
     8101 atggtcaaca agacaatcaa atgtaagatt ttagtcgctg atcatcgaaa aatcggactg
     8161 acatcaaagt tcaagattgc tgatatttca gtctttgatt atttgatcac tgataaattt
     8221 tgttctgaaa aagtattaga ggagatcaga aatacgggga taaaaaccat tcaagtagat
     8281 tcattttaag aaccaggacg taaatatgta aataggatta tcaaaaaaat gaataacaaa
     8341 tttatcgata aatgaaaaac acgctacgct caaattgata aaggaatccc aagatgtatc
     8401 catccaatcg ataccatcat aatctattgc ttcttttata accgttatat gttgcctaaa
     8461 taggaatcaa acaatatcga aaaaaggagg tatgacaaaa gtcacacctc cttttttcga
     8521 ataaacggta ttcaaatcag aacactgatt tcacaaccac tttatttcag gtcgttttca
     8581 atcaccttta ccgcgtaatc atttccgttg aaacgcgcta ttttttttgc gtccatatat
     8641 ctttctaaat aatcatcttc tcctaattgc ttatggcaag aagcaatttc ataaagcaag
     8701 tattccaatg cataagtcga tttatgtttc ttattatact caataccctt ctcacataat
     8761 tggattgctt tatcataatc ctcgatttta cggtaatagg tggcgatatc ataatataca
     8821 atgcagacca tcagattatc aaaatcagtc tcactcaata ttttaagtgt acgctccaca
     8881 taaactttcg caaaatctaa ttctcctttt gacaagtaga tcattgccac gtttgcagct
     8941 gcttgtaagt gatggatatc tgtttcggtc gtttgttcca acacttgatt aaaataaaat
     9001 aatgctgcat ctggtttttt cgcataaaga tgatcaagca gtccttgata aaataaaatt
     9061 tgatttaaaa gatcagaatt acttactttt tcctgatcga tcgtttgtaa aattttataa
     9121 gattcttggt attttgatac agaattcaat gattgaatct catttatttt tttcctgatt
     9181 ttgtcttctt ctgtttctac taatatatcg gagatctcga cattcagctt tttgcaaagc
     9241 gcacttaaga tattgatgcc tggcgacata ttcttgtttt cgatattgct gacagttgct
     9301 tgttcacaaa tgccttctgc taattgtact tgtgttaacc cctttttctt tcggtattct
     9361 tttagtaatg ctccatctac tttaatcaaa acaatttcct tccctttaaa aaattatttc
     9421 tcctttatat catacacaaa aaaaagttct aaacaaccaa aaatgtttag aacagaataa
     9481 aaattttaat tttaaaataa tattcaaatt aaacaactgg cgtatctgct ccttgatttc
     9541 tttttgtgtt atctgaataa ttatcgttta cagttataac acatagtgaa aattgtcatg
     9601 gcgagaaagt ctaagaatat ttttaaacat aactatttct ttcttacgtg tgtataatga
     9661 cgacagcaca cctatcaatt aatataatta ttttattttt ttatgttctc atgtttattt
     9721 ttaatgacac aacttctcgt atttatctgg cacaacatat aaatcgatca aaaaattgtt
     9781 gcgtgttttt ttgaaaattg gagtttcaac ttgattacaa aatttaaatt tagaagttgt
     9841 gaaaacagcg agagacagtt taatattcaa aatatacata ggctactcga acgctgtgtt
     9901 ttttagtata ctagtaagaa acgaagggat agactcattc caataaggag gatttcatga
     9961 agacaattct ttttgctcct gcagtattca atttagctga gacaacacga atgattgagg
    10021 ttgccaaagc tatgaaagac cgttattact gtgtattctt tggcttttct aaaacttttg
    10081 ctcctttgat cacagaaagt ggctttgagt atcatttact agcgccgatc cttacgaaaa
    10141 aacaagaaga acaaatcata aaatttgacc agttgcgtac acttaaaaac ccttttacca
    10201 aagaaatggt tgcctctcgt atcactagtg aacagagtct gattgctaag tttgagcctc
    10261 gactgattgt gacaggttcg aatgtgacaa tttttttatc ggcccgcaat aaaaagattc
    10321 ctttagtata tgttaaaccc tatgctttga gtgaaccttt ttttcaatca gagcatgcgc
    10381 tgatgcccca cgagttacgt ttcttcggcc ctttaagcaa tcccctttgg cactggacaa
    10441 aaaagcgtat agtaaatacc cgatggatcc ccaatgcctt caaacacgct gcacaaaaag
    10501 aaatgcttcc tttaccaaaa acaagtttac aaatgatgga tgctgatcta aatctcatca
    10561 caacgcctaa actatttttg gaagaagaac agctgccgga aaattatcga atcgttggtc
    10621 caatttttgc taagctcagt acttccttgc ctgaagaagt gctaactttt atacagcgaa
    10681 agcaacaaga aagaaaacgt attatctatt tcgcaatggg tagttcaggc aatccgcgta
    10741 tgttacgcaa aattttgttc ttcttacaaa aacaaccgga tctagcggtt attgcaccta
    10801 ttgactatct gcttgatgac attcaatctg actcagaaga tcttttaata acagaatatc
    10861 tgtctagtca tcttttgcat gaattcattg atttttcttt tatccacggc ggggaaggga
    10921 ccgtccaaac tgcctgtgaa tccggcaaac cgtttatcgg tgtggggatg caatttgagc
    10981 aaagctgtaa tattgactat tgtgtaaaat acggaaacgc aatcgccctc acaaaaccag
    11041 tgactgaaag aaaactgcag attgctctgc gcgaagtttc ttcacccaag attcgtcagc
    11101 aggcacgcac gctgcaagcc gtttttccaa taaatggtcc acaacaagca attcaagaaa
    11161 tcgaaacctt cctgagcaac cacccaatat agtttcaagt agaaaaagct tttttaatag
    11221 agactcatca gtaatttcgc tattcgagac ccagacagtg aaaatacaaa aaaagtaaat
    11281 agtggattga aaagttagag ttacttaaat tacggtgtct aaaaaacaca aaatcatcct
    11341 taaaataaaa gatctgacgc tatccaaatg agcataggta gcgtctgcca tttattctta
    11401 gtcaaattct ttgacacctt tctctggttg gatcatctca acgtttcttc aaaagaaaga
    11461 ccataataat taggcaaata cattttctta aaatgtgtca tatagatcta gaagcatcgc
    11521 tttgtagaga tttgtgccgc tagtcccctc aacaattact aaatcaaatg taggatttac
    11581 cactttatct ttgataatca acctccctct tctgttgaga caatgatctc gcaatccgat
    11641 gcatactcct atataaactg ccagcaatcc ccacatattg agtagatcat gtcagaatgt
    11701 tctatgtcgt cagcaacgat acactaaaag tcacgttcac catgactgat tgctattccc
    11761 agcacaatta tttctataca cgtttcatga tttgaataaa cactcacatc atacatatta
    11821 ccattcatac accatactgc agcccctact atatggttgt aagctcccaa atctatattt
    11881 gacttgatca ctttggtagt agatctacta attctaaatc cattgctgat aaaatttcca
    11941 ttttttcctc cttctttctg ccaaaacagt taaaacaata ataatcttta tgtaaaaaag
    12001 ggctgtgaca aaagccttgt cacagtccca tattccgaat aaacggtggt caaaagcttg
    12061 catctgcggt aataagttca accaaacgaa aaataagcca acatttcaca aaaatttaag
    12121 aagcaatttt cgtaaatttc ttcttatttc ttgaagctaa ccacttctgt tacaaccact
    12181 tttagaacat ttaaagcttc gatgtgacaa accatctatg ctgctcgatc ataaaattga
    12241 tagcaaaaat tccacaaagt acatatgtcc cccatgaaaa caaaacattc atatgcggta
    12301 caatgatctt tggaagaatc acgatcaaaa atgatgccag tgcccaaatc catcgattga
    12361 tttgaatatg atatgtagct agcatattca atataaggtg actagccgtt aataaaaaga
    12421 taaaaacaaa aaataaaatc agccaataca ttgtttccca ccttctaaaa accaaactta
    12481 gacagtctgg acttcgtttt ctccacgact ttgttcatct gacagcatct ttttatcata
    12541 agcttttaca aatggataat agataataaa tgctatcaat gtgttgatca tgatcaagat
    12601 aacaacacgc cagtcatttc ctgctgaaag ataaccgccg attggagctg gtaatgtcca
    12661 tgttggcaaa acagtaaatc tattgaccat ttctaatttg actgcaaaat aactgataat
    12721 agtcaaaact aaaggaacta agttgaacgg gatgaacatg taaggattca acatcatagg
    12781 agctccaaaa attatgggct cattgatatt gaagatacca ggaataatag aaaaacgacc
    12841 aacctctttc aagaattttg atttacagaa gaatataaac aaaacagata ccacgatcgt
    12901 tgctcctgca ccaccaattg ttaccgtcca ttgataaaac tgttcaggag cgatataggg
    12961 taattgggtg gcagctgtac catctgccaa tgcctttgca ttctcatcca acatgatcag
    13021 ccacattgga cgaacaacag cccctacaat actcatgcca tggataccaa atgaccacaa
    13081 caaatggatc aaaaacattg gtagtaaaac accaagcaaa ttattcccta aaatattggt
    13141 taaaggctcg aataacgata ataaggcagc gtttacatca aagcccgcga cgcttcgaat
    13201 cagccaagta attgttaaaa tgactgcacc tggaatcaat gcctcaaaac tacgggcaac
    13261 agcaggtggc acttgttctg gcattttgat agtaatattt cgatttttaa agaaacgata
    13321 tacttccact gcaaaaatca tagataagat ccccgcaaac atcccagatg ctcccaaaga
    13381 actcataggt aagacccagc caagaggtgc ctcttcagga gttaaaacgt ttacaggaac
    13441 ttgtaacata aagaacgttc ctaaagataa tataccacct gtcacactat ctaatccata
    13501 tgatttagct agatccgaac ccataacaaa agttgcataa atagccatca atcccatcgt
    13561 catcctaaag ggtaaaacaa tcgttgccga ataaggggca ataaattcat tccatccagg
    13621 aaacggtaaa ttgataaaga ccataaaaaa tgtcccaatc aaaatcaatg acaatgttga
    13681 aacaacccca ttacgaattg ctaataaatg acgctgtgta ccaatttttg ccattggtgg
    13741 gacaagtttc tcttcgaccc atttcatcag cttttccact aaaaccactc cttttcaata
    13801 aaatatgaac cattcattgc atgacaacgc aaagaatgtt cataactaat tatataatta
    13861 agaaatttta tttcaatata ttctcttaaa taatcaaatt tatttttaag aatcctgaaa
    13921 ataagttttc taaatcatag gagcaacaaa aaaattcgat aattaacaaa aatcaaaatg
    13981 attaaatcta gtgattgaaa catatcattt caaaaaaatt ttgtaaattc acccataaat
    14041 atcaccctat ttaacttttt tcatcgtaca ctaaaattca aaacaactat ctttaatctt
    14101 tacttcttgg ttttgtttta ataaatttcc ttcaaaaatg cctggagaaa ctacaatgat
    14161 caccgtatct ttttgtgctt gctccaactt cttcaaatct ataatagcta acagtgtccc
    14221 ttcttttacc tcctggttac tttcaacccg tatctgaaac ggacttcctt cgagttcaac
    14281 tgtatctaaa cccatatgga tcagtatttt tctgccagat tcactttcga tcgttatggc
    14341 atgtcgggta ggaaatatat tatcgattat tcctttaact ggcgcatgga tatatccatc
    14401 atgattcgtt atagcgtatc caactcctat catttttgaa gaaaagacct cgtcttttac
    14461 tgctgttaat ggaataattt gaccactagc tacagctttt agaatgtttc ttgttttaaa
    14521 gaaatgcact ttttatcacc tctactttaa tgctggccaa tattttttgt ttactttgat
    14581 catttcatct agtatttttt ttgcgactct ggcatttggt acggtttgat tcaatgtaaa
    14641 tgcttgtaaa gctttttgat aagaaccttc aaagtaagca tctactagta acttctctac
    14701 tgcaacttgt gcctccatca atcctttatg aaaatcactg attggttttc ttagtgtaat
    14761 tggctccaca ccagttgcac caacatatgc cggtatttca acaactgcat ctgaccgtag
    14821 attcggaatc gccccttgat taggaacgat tagcataaag cgacgacgat catcattcaa
    14881 caaggaaata gctatatcga cgatatattg tccatgctcc ccaaaattaa agttcaatac
    14941 ttcttccaca ataccgcggc gtattttatc agccatttct ttggtatttt tctctcgtcc
    15001 atccatgacc atattagcac gtgtatactc tttgtcagta tgctcaacga ccatatcagg
    15061 atataagtaa tactctaagt aattattcgg gatattactt ggaaaatttt tcgtgatgac
    15121 tgacatcatt tgaaaggttt tttgccaagt tttatctcct atattaaaat cggcgacttg
    15181 catttctttt gttattagtt tttcaataat ctctggcata atttctcgtt gcaattcttt
    15241 atcataaatc gatgtatacc agccaaagtg gtttaaacca taatacgtaa cgatccaatt
    15301 cttacgatca taaccgtaat tcactgcaat cgtttcttca attgaaatag tcatatcaca
    15361 ggcattaatc atttttacat tcggatattt acgacgaaca gcttcagaaa cgatcgactc
    15421 tggatttgta taattcaaaa tccatgcttt gggggcatag tcttgtatat gatcgacaat
    15481 ttccaataaa cctttgattg agcgcatccc ataagcaaat cctccgagtc cacacgtttc
    15541 ttgtcctact aatccatgtt taagtggaat cttttcgtcc ttttctcgca tctccaatcc
    15601 tccaacacga atttgtgaga aaacaaaatc gcatcctgta aaagcaagct ttggatcatc
    15661 cgttgcacga atgcgaatat ttgaaaaacc ttttcttttc agcataaatt taacaatcaa
    15721 gtacatatcc atatttcttt cttcatcaat atcatataaa cgtatttcag ataatggcaa
    15781 ccgtttttca ttgttcaaaa tggcttgaac gattcctggt gtatacgtac tcccgcctcc
    15841 agctatagtg ataatctgtt ttttcatcat catcttttcc tttctattct tctaataatt
    15901 tattgaattc tgccataaca taagtcactt ctaagccgat gataatttga taattttttt
    15961 cagttggttt aatgatcccg cttatcccta attgcttgat tcgctgttcc tcgaccatgg
    16021 tgggatccac tacctctacc cgtaggcgtg tattacaact atagacatcc aggatattgg
    16081 cttttcctcc aaacgcttgt agcatttttg ttgccatata ttcataattt ttacttgcta
    16141 accctaactc tgtttcttct aaggttactt cattgccaaa ctctgcctta gcttcacgtc
    16201 ctaatgtctg aatgtttctc ttattgatca aataataaaa agtaacgtaa tacaaggcaa
    16261 aaaagaaaat tccgattgga agaatcaaaa tagcattggt agcgattcga aaattgatga
    16321 gataatcaac aatacatgct ccgaatgtaa aaccaattcg tatattgaat aagtaacaga
    16381 caatccctgc taaaccggca aaaagtgcat ggatcacgta caacatagga gaaagaaaca
    16441 tgaaactaaa ttcaacaggc tcagtgatat ttgcaataaa tgaagttcct gcaccgcttc
    16501 ccatgattcc tttgaccatc tctttatttt ctttataagc tgctttatag atagctagac
    16561 atatagctgg tataccaaac atcattacta caaaaaaaac tgataaaaac agtccagcct
    16621 gaggatctcc gttgataaag cgtgtcatct ctcctctgaa aacttccttg ctaggagatg
    16681 tatagcttcc taaatcgtaa taaatatacg tattcaacac atggtgcagt ccaaagggga
    16741 tcagcaaacg attcaaaaac ataaatatcc ccacacctaa tgctcccata tcaacgagca
    16801 gttttgcaaa attatcaatc ccaatttgga tgagcggcca gataaagccc aaaaaaacag
    16861 ctgcgattgt ttgtagaatc atcaccatag tcaatgggaa tttttcgccc gcaaaataag
    16921 aaaaaactac tggtaatttt tgttctttaa agcgattata cgtccatgcc gctactaaac
    16981 cggaaataat tccaccaaaa atccccatat tcacttctgg gtccataatt tctaagcctt
    17041 ttttcaaggt agcaatcgct aaaaaaccag taagtgcagg tattcctttg tccttatttt
    17101 tgacaaaacc gattgcaact cctatagcaa ataagatatc caagtttcca aaaacggcat
    17161 ttccagctgc accaattact gggatgttca gcatatcgtc agctgataaa cgcatgatca
    17221 atcccgctaa tggcatcgct gcaactggaa gtagcatcgc tcttccaagc ttctgcacat
    17281 ttgatttaac attcattttt attcctcctc tattaaatat cctcatatgt tactaactca
    17341 atcaaaggat catttttcac tttttgagca aatgctgaat gaattaaaaa ttcgacttgg
    17401 tgggtacgag gatacacaaa acttgaacca tttaacaatt cctgatccaa atacgctgcg
    17461 tgacacatga cttcgatcgt tttatcttct ctcaatatca tgagtaaact tgccaaatat
    17521 tcttccacac tcgtattgat ttgtcgtttt ttttcatcag tttctcctac gacatcgaaa
    17581 caaggctcaa aataatcgac atgacgaact tcatcttttc gttcaaagtt gctccgaacc
    17641 ggcaaatcat atttttttgc taatgtaaca actacctcct gattttcatc caaagtatga
    17701 gtatgatgat gactgtctaa atgagttggt atgatccccg catgataaat tttttgaatc
    17761 tgagcattcc attcctgata cagttgatct ttgtcaatgt taaatggatg ctgataataa
    17821 gataaggatc tgaactctcc gctttcgata agagtatcga cagttttgag gagtggtttg
    17881 ccacacgtca atgttaaatg gactcctaca cctagcctag gcatttcctt tcttagtttc
    17941 acagcatgct cgaaacccgg catatttgcc atcaatgtag ttgatgtcaa aattcctcgc
    18001 tgataagcat ccataatccc ataattcaca ccatggctat agccaaaatc atccgaatta
    18061 aaaatcactt ttcccatttc attctccttc ttttttctac taaaataggc gcaaccaaat
    18121 aatcatagtc catttactat gtgtcatttg gttgcgccta tagatagtta cgcccttatt
    18181 ttaatagatt cttgctagat gaattgccaa atagatgatt tcattttctt gaatggcaag
    18241 ttgaaattgc tgttcgatga tatctttaat ctttagtgct gtacaatacg ctttgctatg
    18301 tttaaatgtt tcagtaaaaa aaccaatatt ttctactggc gtttcttcct gataaatcaa
    18361 gcgtttaacc aggaatttga tatgaaccat taagcgatga taagtcagtg attctttatc
    18421 ataaacgttt gaataggatt tttctagatg ggacaaaatc gtggtcgtca tttctactat
    18481 ttttaagctc tttaaactat taggctctcc tagctcagca ttaatgatat gcatagtgat
    18541 gaatcctact tcatttgacc ctaactcggt ttgatatcga tgattgattc gctcgaccac
    18601 ttctactgct atatcatatt catttggata aaggaatctc atttctggca acataattgc
    18661 tgtgacatct ctatcttctc tttttcgttg aatagcaaaa taaatatgat ctgtcaatgt
    18721 aatatagata tttgaatcta atggagagga gatcctagct gttatcagtt caatcacttc
    18781 ttccgcaaaa tccataaatt cgattggaat ctgcgagact atctcactga atcgtttgat
    18841 cttttcttta gaatctaata caaataattt gtcagcatcc tcaacatcaa atggcttact
    18901 cactttagca gaaaagccaa tccctttccc catcgcaatt ttttctattc catcgtcatc
    18961 gatcaataca gcattatgat tcagcttttt catcacatac accgttaatt cacctcctct
    19021 atatgataaa acaaaacaac ttgcagactc cttcatacat atttttgtct ccaaacaatt
    19081 tttaaagaca aaaaacgacc taagaaagaa ccctttctta ggtcgtgcct aattgaatag
    19141 tagcacccta cataatttac aaaaatagtt taacacataa taaaagcgtt atcaatatct
    19201 tttttactcg ttttatgata atacttgtat cgaattgatt tggaggtaat tacataaaaa
    19261 caaaaaaaat atagcaactc tctataagaa agttgctata ttgataatta taccggcggc
    19321 cggggtcgaa ccggcacgtc ctcaacggac actggatttt gagtccagcg cgtctgccaa
    19381 ttccgccacg ccggcaaatt taaaaataat aactggggta gctggattcg aaccaacgca
    19441 tgagggagtc aaagtccctt gccttaccgc ttggctatac cccaagaata aagggcgaat
    19501 gatgggaatc gaacccacga atgccggagc cacaatccgg tgcgttaacc acttcgccac
    19561 attcgccatc ataaaagatg ttatttaatt tctcaataaa aaactaagat gaagacatct
    19621 tagtcaatac cggcggccgg ggtcgaaccg gcacgccctc aacgggcact ggattttgag
    19681 tccagcgcgt ctgccaattc cgccacgccg gcaagttgat ttattagtac taaggcggta
    19741 accggatttg aaccggtgat gaaggttttg cagacctctg ccttaccact tggctatacc
    19801 gccattatac tattatcaat ctataaaata aagaataaat tatagaaact ggggtagctg
    19861 gattcgaacc aacgcatgag ggagtcaaag tcccttgcct taccgcttgg ctatacccca
    19921 agaataaaaa gggcgaatga tgggaatcga acccacgaat gccggagcca caatccggtg
    19981 cgttaaccac ttcgccacat tcgccatggc aggggcagca ggaattgaac ccacactaac
    20041 ggttttggag accgctgttc tacctttaaa ctatgcccct ataaaatgga ggagagtgga
    20101 ttcgaaccac tgaaccctaa ggaacggatt tacagtccgt cgcgtttagc cacttcgcta
    20161 ctcctccatg gtggcgcggg acagaatcga actgccgaca cacggagctt caatccgttg
    20221 ctctaccaac tgagctaccg cgccatattt taaaattatt aattgaataa caagaacgac
    20281 ggttccgacg ggaatcgaac ccgcgatctc ctgcgtgaca ggcaggcatg ttaaccccta
    20341 caccacggaa ccagttcatt atggaggtta acgggatcga accgctgacc ctctgcttgt
    20401 aaggcagatg ctctcccagc tgagctaaac ctccaacatt caattaaaat gacccgtacg
    20461 ggattcgaac ccgtgttacc gccgtgaaag ggcggtgtct taaccgcttg accaacgggc
    20521 caggtaaatt tgctacggag agtaagggat tcgaaccctt gagacagttt ggcaccatct
    20581 acatgatttc caatcatgct ccttcggcct ctcggacaac tctccagagt gaaagaagct
    20641 caatactctt tctcaaaatg agaactccgg cagtaggact cgaacctacg acatcatgat
    20701 taacagtcat gcgctactac caactgagct atgccggaat aacatcagcg tggcgacgtc
    20761 ctactctcac aaggggcaac ccctcactac aatcggcgct aagaagctta acttctgtgt
    20821 tcggcatggt tacaggtgta tccttctcgc tatcgccacc acactgtggt gttatctttt
    20881 attgagtaaa ttttgttcac tcaaaactgg atttgaagtc acataagttt ttttccgagt
//
LOCUS       DASHHB010000048        20936 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.050, whole genome shotgun sequence.
ACCESSION   DASHHB010000048 DASHHB010000000
VERSION     DASHHB010000048.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20936)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20936)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20936
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.050"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(227..1114)
                     /locus_tag="U2K71_002031"
     CDS             complement(227..1114)
                     /locus_tag="U2K71_002031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM7316064.1"
                     /translation="MKNMVEKETIERPNVKKQALELRSTIGSVLKYTALSILSFISIF
                     PFVWMVLGMTNTPVDITSGKIKIGSNLIINFQNLFSNELNFTRSLWNSAMIALVTTVF
                     ALLISSMAGYGFEIYQSKRKDLIFNILLLSMMVPFAALMIPLYRMFSQLNGTVLGINS
                     FLVVILPSVSTAFLIFFFRQNVKSFPKDLVEAARLDGLKELSIFFRVYMPTAKNTYAA
                     AAIITFMSSWNNYLWPLVALQSPDKRTVPLVLSAMGASYTPDYGMIMAGLVIATLPTA
                     IVFFVLQKQFVQGMIGSVK"
     gene            complement(1111..1980)
                     /locus_tag="U2K71_002032"
     CDS             complement(1111..1980)
                     /locus_tag="U2K71_002032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628995.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7316065.1"
                     /translation="MKQKDLFKNGHAFLILPVILISIMVFIPMVLALITSFQSGPPVN
                     MTFNGLGNYQRMLTDATFKKAFFNTFLYLLVQVPIMLFLALFISNLLNDKKLKYKGLF
                     RTAIFLPCITSMVSYSLIMKSLFSQSGLVNNLLLGIGMIDTPIGWLTHPIWARVLIIL
                     SITWRWTGYNMIFFLSGMQNIDSEIYEAASIDGASKWQQFIKVTIPNLKPIILFTSIT
                     STIGTLQLFDEVQNITGGGPANATTTLSQYIYNLSYKFTPNFGYAAAVSFVIVSFIVI
                     LSIIQTKVGGESR"
     gene            complement(2117..3403)
                     /locus_tag="U2K71_002033"
     CDS             complement(2117..3403)
                     /locus_tag="U2K71_002033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter substrate-binding
                     protein"
                     /protein_id="HEM7316066.1"
                     /translation="MNIRKLIAGVVCLAAASGFLTGCGSASSTADKEEKVTVWAWDET
                     FNIKAVNEAKKVYENEETEIEVVTMSQDDIVQKLNTALASGNTEGLPNIVLIEDYRIQ
                     GYLTSYPDAFANLSAIVKEDHFAPYKFAVNKVGDKIYGVPFDSGVTANFYRTDLMAEA
                     GYTEEDMENLKWDEYIQVARDVKEKTGKKIAEVNPSDLGRVRMIMQQAGEWYTSEDGE
                     TVTIQDNASLKYGLELFATLLKEDLVEQTSDWNAGVTAIQSGAVASSPTGAWYSSTIQ
                     GAENQSGKWKIAPIPALPENLQKAQASNLGGAGWYVIKGVAGEDSAKDFLEATFASNE
                     ELMGTLAKEIGLVSTMLSANEQEAYQEASEFYSGQKIFDDFSTWTSEIPEVNYGHETY
                     AIEAVVAEALHRIINGEETDKVLADTQKQVEAQLAN"
     gene            3676..4254
                     /locus_tag="U2K71_002034"
     CDS             3676..4254
                     /locus_tag="U2K71_002034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HEM7316067.1"
                     /translation="MPIRVILIDDEQTILDGMKQLINWSEHGFQLIGTFSSPLKALDF
                     SLVHAIDIVVTDVMMPELNGIELSRLLKQQQPETQILILSSYDEFDMVKDSFKEGVAD
                     YLLKPRLSPDFFLNSLTAVSQKIKKKPNKSPITNRYEQICENLSQQIAGQTRPLLDPL
                     LFEHTLFFCYIPKNGHSVQNKALNKNSRSPIH"
     gene            4254..5183
                     /locus_tag="U2K71_002035"
     CDS             4254..5183
                     /locus_tag="U2K71_002035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7316068.1"
                     /translation="MDSDDSFTIYPVSTFSEESGYVINTDSKDTLRKFVQKFRQEVFP
                     GDFFVVSDSFAIQDINEIFQEIRNCTKGQRFYHKGKNLAYQHELLPLLTNHEQQASSY
                     LTKIVHKDYALAVKELENFFTTILEQPIDPAYLKHEAINRFYALLNALADEHVGNEEL
                     SLLKIALPSLLADAEYFEDFSILVKETLEKLTHFSANAKKDNFDILTLIYEFVQENYE
                     KEINLQLLSEKYHFSYSYLSAIFTEKFGINFSKYLKKVRISKAKLFLTETRLTLTEIC
                     EKVGYTELGYFSRVFKEETGMTPSQYRKGTFVK"
     gene            5180..6916
                     /locus_tag="U2K71_002036"
     CDS             5180..6916
                     /locus_tag="U2K71_002036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HEM7316069.1"
                     /translation="MRKKIKEHELFAKLLFILFIGLFLQAIVISLFIYHRSRDAYIQL
                     FNQSNDVVLKKIQSEFESLNDTIENTLAAFDSNPAVKSYFSNDPAQHMEQLQQLRTIQ
                     KMNDSLSKIHPMIDYDVLIFGENGRTFVGNDMLTAVSADSFFQSAIAQRVNERAADTQ
                     MLFAHHGLTLRDKKAPSVFFVKKLKNTLNHVYGYAVLSISSKQLANLFQSVVNPEISK
                     ISFMNEEQTIIASNEENTIGSRSMIFKKLTVGETTISNGQQLTRLPLYRQDCTLLSQV
                     DLHSLSNQMGVIFPIILYNIVTLAFAGSLVFMYLDRHTKSIYRLIDSLKRLEEDGLKA
                     TVPVQGSHEVQLLGNTINQLLSNAQHHYEQTIQDEKKKRSLEIQTMQAQIQPHFIYNT
                     LTSLKFLIWQKKTDEAVQGIDSFITLLRSTIGKKEEVIPVKEEIKSVQSYIDLLSMRY
                     GERISAKIMIPDELQSLSMPNMILQPIIENAYLHAFHNKTQGFITVYGKTKNQLLLFE
                     VIDNGDGFDTENTKQKNDHFSGIGISHVDERIRLMYGDAYGLTIQSVIGTGTTVTICL
                     PLLPKNQSEYQK"
     gene            complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="U2K71_002037"
     CDS             complement(6979..7815)
                     /gene="pheA"
                     /locus_tag="U2K71_002037"
                     /EC_number="4.2.1.51"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydratase"
                     /protein_id="HEM7316070.1"
                     /translation="MNVSYLGPESSFTYQAACQLFPTEQLTAYASIPVCLRALFRKQV
                     DLAVVPVENSLEGAVHHTIDLLSKHPEVEVKSEIVLPIKQQLLGNPATKITKILSHPQ
                     ALAQSQQFLETHYPNVPLVATESTTAAAMYVAEHPKEDAAAIASLETAQHVGLEILAE
                     NIQDNELNQTRFWIVGDRKMTSQQSAPVKMSVILTLPANRPGMLHKMLAAFGWREINL
                     SKIESRPLKTSLGEYFFVIDLLLDRPMTLVENALQEIKMLGGESQILGCYPVLTVEET
                     RR"
     gene            complement(7812..8321)
                     /locus_tag="U2K71_002038"
     CDS             complement(7812..8321)
                     /locus_tag="U2K71_002038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HEM7316071.1"
                     /translation="MASILLIGFMGAGKTTIGKGLAQCLQKEYVDLDTKIEEHIQLSI
                     AEYFHYYGEKSFRKVESDILRKLSNEDKIIATGGGIVQSAENRRFLKTQPIVLYLEAE
                     ADCLVDRIQQDETSIRPLALGKTREEIKALLAQRLSWYEESATHRINTTNQTPEKIID
                     TIIERIKSI"
     gene            complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="U2K71_002039"
     CDS             complement(8314..9609)
                     /gene="aroA"
                     /locus_tag="U2K71_002039"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HEM7316072.1"
                     /translation="MQLLQQIHGLRGTVRIPADKSISHRSIMFGAIAEGTTTIQNFLR
                     AEDCLSTLHAFQQLGVEIEEEEEVIKIHGRGSHSFVQPTESIDMGNSGTTIRLLMGIL
                     AGQPFTTTLFGDASLSKRPMGRVMEPLREMGADLQGEENDQYLPITVTGTRSLSPIRY
                     NMPVASAQVKSALLFAALQAEGTSVIVEKERSRNHTEEMIRQFGGRITVEDKTIIVTG
                     PQKLTGQQITVPGDISSAAFFLAAGLLVPESQLLLKNVGVNPTRTGILDVLEEMGAAI
                     TQTNHNEQNQSADLSVKTSHLKKATIGGEIIPRLIDELPILALVATQAEGITIIKDAE
                     ELKVKETNRIDAVAEELQKMGAKIKATADGLIIHGPTPLHGAKVSSRGDHRIGMMLQV
                     AALIADGPCELEGAEAVSISYPAFFDDLAELVSGGESHG"
     gene            complement(9622..10713)
                     /locus_tag="U2K71_002040"
     CDS             complement(9622..10713)
                     /locus_tag="U2K71_002040"
                     /EC_number="1.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase"
                     /protein_id="HEM7316073.1"
                     /translation="MAQHVFIVGLGLIGASMALCIRQTNPELIVVGWDSQSKTREQAV
                     EQQIVDYVASDFETGAEQADVILLAVPVRTTLAYLDVLERINLSDHVIITDVSSTKQQ
                     VVTSAEQKNLRFVGGHPMAGSHKSGIQAADAKLFENAYYIFTPLEKTKKDVEKLQELF
                     CGTKAKYVTLTAREHDRITGMLSHFPHILAAGLVNQAEQFNQEYPRAKQLAAGGFRDI
                     TRIASSDPVMWTDILLSNKQILLERLADWQQEMTQIAEWIMTENQSEIFSFFNRAKES
                     RDQLPVHKQGAIPAFFDLFIDVPDEPGVIAEVTGLIGKAGVSLINLKIQETREDILGI
                     LQISFKNQQDLLQAKRIIMSNTSYHCWIK"
     gene            complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="U2K71_002041"
     CDS             complement(10730..11896)
                     /gene="aroC"
                     /locus_tag="U2K71_002041"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001269368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HEM7316074.1"
                     /translation="MRYMTAGESHGPELTAIIEGLPAGLPLSEEEINHELARRQAGYG
                     RGGRMKIEKDRVRITSGIRHGKTIGSPVTLVVENKDWKNWTTVMSVAEVPEKDQSLRR
                     VARPRPGHADLVGGMKYHHRDLRNVLERSSARETTMRVAIGAIAKKILEELDIIVAGH
                     VICLGGIQAEIPGNISIQEIKEISEASEVRVVDPAVEQRIKDLIDQTKKNGDTIGGIV
                     EVLVGGVPAGLGSYVQWDTKLDAKIAQAVVSINAFKGVEFGVGFTSGSLPGSEVMDEI
                     LWEEERGYFRRTNNLGGFEGGMTNGEPLVIRGVMKPIPTLYKPLQSVDIDTKEPYKAS
                     VERSDSTAVPAASVVAENVVATVVATELLSKFPSDSLVELKAAVKEYRDYLKHY"
     gene            complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="U2K71_002042"
     CDS             complement(11898..12962)
                     /gene="aroB"
                     /locus_tag="U2K71_002042"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HEM7316075.1"
                     /translation="MLTVHVDNQKYDIHIEKGALEQAGSWLQTIWRPQKVAVITDTNV
                     APLYAEKIMFQLRQANFEPVLSIVPAGETSKSLEQAAELYDFLADNDFTRSDGILALG
                     GGMVGDLAAFVASTFMRGIHFVQIPTTLLAQVDSSIGGKTAVNTNKAKNLVGTFSQPA
                     GVLIDPEVLKTLPVRRLREGIAEIIKSAAIADATLWKQLNELKDEKDLLEHAVPIITA
                     TLEIKRKVVEEDVFDQGNRLILNFGHTIGHAIENTAGYGVVSHGEGVAIGMVAITSHA
                     EKIGLSPTGSTAALERILQKFHLPITYDLSHPDELFHAITHDKKARGSQLNIILLEKI
                     GQAKIVPVPTETFKDYLGGL"
     gene            complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="U2K71_002043"
     CDS             complement(12995..14017)
                     /gene="aroF"
                     /locus_tag="U2K71_002043"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HEM7316076.1"
                     /translation="MIVIMKKEATEEQIKKVVDHIEKEGFKVSIDQGTERVVIGLKGD
                     TRSLQEGAFTRYEGVENAVRILNTYKLTSREFHPGNTVVDVDGVKIGDGSFVTMAGPC
                     SIEGLDQIRECARMAKAGGAKILRGGAFKPRTSPYAFQGLEEEGLKYIRQAADEFGMK
                     VITEVMDEGHIDMVAEYSDILQIGARNMQNFKLLSAVGKTGKPVGLKRGISGTINEWL
                     NAAEYIAVEDKSPVIFIERGIRTYETATRNTFDLSAVPLMKKLTHFPVIVDPSHGTGI
                     WELVPPMARAGVASGADGMIVEIHPDPANAWSDGPQSLNEKTYSRMMKEVAIMKEAME
                     KIQTLD"
     gene            complement(14676..15650)
                     /locus_tag="U2K71_002044"
     CDS             complement(14676..15650)
                     /locus_tag="U2K71_002044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase small subunit"
                     /protein_id="HEM7316077.1"
                     /translation="MKNTVNKSHMDTEKVAIVFGDCTLGVKSGNTHYIFSYTRGGLES
                     LNKNGKEWLYRETLPVFWRATTDNDRGSGFGFKSGMWLGAGLYPKVKEIEVLVDNKKI
                     DLPIAPVNNQYSSNEYAETVEIIFTLEINTVPKTQVTISYQIAGEGKMLVNMHYYGQK
                     GLPELPALGLRFIMPTKAKFFEYEGLSGETYPDRMAGGVEGIYKVAGLPVSPYLVPQE
                     CGMHMETKWVELTRNQTLDNTDKDQEDFSLRFEQQDTPFAFSALPFKAEELEQATHIE
                     ELPPERRTVLTIFAKVRGVGGINSWGADVESPYRISSEEDHEIGFIIQ"
     gene            complement(15631..17514)
                     /locus_tag="U2K71_002045"
     CDS             complement(15631..17514)
                     /locus_tag="U2K71_002045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 2"
                     /protein_id="HEM7316078.1"
                     /translation="MKANIKWLDDPQVFRVNQSNAHSDHVCYATREEVETTSSLKQSL
                     NGSWRFCYSPNPSVRPKTFYKKDFDACSFDEITVPSHIELANYDKIHYINTMYPWEGH
                     VFRRPPFTTSPEKEAEGIFSAAKDNPVGSYLTTFRLEEGLKGKQISIHFAGVEQAMFI
                     WLNGHFIGYAEDSFTPSEFDLTPYLEEENILAVEVYKRSTAAFLEDQDFFRFFGIFRD
                     VFLVARGQNHLEDLWIKPRLDDKCQNGKLEVDLSFEQVAKNSQLNVAILDPDGKEIYQ
                     QQYPLKEQMTIRIDGFENVALWGHFQPNLYKLELETIDEEKRVTEYIDYSFGFRKIEI
                     KDKIIYFNGKRMIINGVNRHEWHPKKGRAITIEDMEKDLAIIKNAKINGVRTSHYPNQ
                     ITWYYLCDQAGIHVMAETNLESHGSWQKMGAFEPSYNVPGAVPQWQAAVLDRAKTNFE
                     TLKNHPSILFWSLGNESYAEENIRQMNDYFKKVDPDRLVHYEGNFPNPAFEDSISDVK
                     SCMYAPPEEIIDYLENKAEKPFILCEYMHDMGNSLGGMDSYMTLLDRYEMYQGGYIWD
                     FKDQAIQVTDPITGREVMRYGGDFDDKPSDYEFSGNGIVFADGQEKPAMQEVRYYYEK
                     YSK"
     gene            complement(17532..19757)
                     /locus_tag="U2K71_002046"
     CDS             complement(17532..19757)
                     /locus_tag="U2K71_002046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HEM7316079.1"
                     /translation="MDITHRKTDKNFILFDEKSRVFHLRNTFLSYLIKIEESNVLAHI
                     YFGKPVKQYKDNKNYPRRDRGFSGNVPLNPDRSLSKDTLPQEYSSHGSMDFRTPASII
                     QRKNGSDLLDLRYDSHYITDGKPEIEGLPQTYVMDKSEAQTLVISLKDRETAIYFDLF
                     YTIFTDRAVITRSVKIRNETGETIKLEKAASFQLDFAHTRRFDEVIALPGAHVNERQI
                     SRQSVLSGTKVFESRRGTSSHHMNNFIALVHHHTTENTGEAIGLQFVYSGNHSFELEK
                     DQINQLRVVGGINSHRFSWELNAGQSFQTPEMILSYSSQGLNKMSQIHHELLRERIAR
                     GRHQFAERPILVNNWEATYFDFNSEKIKAIIDEAKELGIEMFVLDDGWFGKRDADNSS
                     LGDWFEYEGKLTNGLREIADYAHSKGLKFGLWFEPEMISVDSELYRTHPDFLMQEPGR
                     MPSASRSQHVLDFTRLDVRQTIEKQMRKILDTIPLDYIKWDMNRSLSDVYSITLDPQR
                     QGEVAHRYMLGLYELLEHLTTDYPEILWEGCSGGGGRFDAGFIYYMPQSWTSDNTDAV
                     ERMKIQYGTSLAYPISSITAHVSAVPNHQTDRSTSLKTRGETAMSAVFGYELDLTKLS
                     PEEKKQVKDQIISYQTIRPVIQYGHYYRLASPFEENIAAWMFVSPKQDEAIVFLGRIL
                     ASAQPAFHEVYLMGLDDEALYQEQTSKRIFSGAELMTVGLYFPDFQGDFQTELLHFKK
                     L"
     gene            19872..20906
                     /locus_tag="U2K71_002047"
     CDS             19872..20906
                     /locus_tag="U2K71_002047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7316080.1"
                     /translation="MVGIRDVARKANVSPATVSRVLNNDQTIHVAQTTRQRIVEAAAA
                     LDYDISTRKYIKKRMPSIGVISTIGKASEEQDIYYKHLRVGMEEEARRLHLGMNRIYN
                     LSDNPKQWEDLDQLGAVIVVGTVTKESITALLKQNMQIVVVDNPDIQQEVDMVYADLE
                     RMTKTVLELFLENGHKRIAYIGGYKVDMDEFGGKQISINEKRMRAYQHFMIDHHLEKY
                     MSYHLGGWTEKQGEVLATNLLENESPLPTAILVGSDPMAIGVYRALKQKNIQIGEEIV
                     VASFDNIETIANLSPGLTSVQINAKSIGQAAVRMALERIDGIRDEPIILTYPARLIVR
                     ESFIPRTKRY"
BASE COUNT         5950 a         4506 c         3822 g         6658 t
ORIGIN      
        1 gacaatcagg acagtcatcg gcaatatagc agcaatcact tctttaaaat tttttagcat
       61 gtatcctcct cctttactct ttaagtttac cttaaaatga aagagttttc gagcacaaac
      121 ttaaaacgtt atcatttaat ttttctcaac ataaaataac acacgtccca aaagtatctt
      181 tttacttgga aaaatgactt tgaaggaacg cgcttattgt gtttttttat tttacagagc
      241 cgatcattcc ttgaacgaat tgcttttgca aaacaaaaaa tacgatggca gttggtaaag
      301 tagcgatgac taatccagcc atgatcattc catagtctgg tgtataggaa gcacccatag
      361 cagataaaac aagcggaacc gtccgtttat caggagattg caaagctact aagggccata
      421 aataattgtt ccagctactc atgaatgtga tgattgcagc cgcggcatag gtatttttag
      481 ccgtcggcat atacactcta aaaaaaatac ttagttcttt tagaccgtct aatcgagcgg
      541 cttctactag atcttttgga aaagatttaa cattttgtcg aaagaagaag atcaaaaaag
      601 cagtgctaac agatggcagg atgacaacca agaaactgtt gattcccaaa accgttccgt
      661 tcaattgtga aaacattcga tataaaggga tcatcaatgc cgcaaaagga accatcatcg
      721 atagcagtag aatattgaaa attaggtctt ttcgtttact ttgataaatc tcaaatccat
      781 atcctgccat tgacgaaatc agcaatgcaa acacagtggt caccaaagcg atcatcgctg
      841 aattccaaag ggaacgagta aaattcagct cattggaaaa caaattttga aaattgatga
      901 tcaagttact gccaatcttt atttttcccg atgtgatatc cacgggtgta ttcgtcattc
      961 ccaaaaccat ccagacaaaa gggaaaatgg aaataaatga taggatgctt aatgctgtat
     1021 actttaatac tgacccaatt gtcgaacgaa gttctaatgc ttgttttttg acgttggggc
     1081 gttcgattgt ttctttttct accatatttt tcatcgactt tctcctccta ctttcgtttg
     1141 gattatcgac aatatcacaa taaaggaaac aataacaaag gaaactgctg ccgcatatcc
     1201 aaaattaggc gtgaatttat aactcaaatt atagatgtat tgagataggg tggtcgttgc
     1261 atttgctggt cccccgccag taatattttg tacttcatca aaaagctgca atgtcccgat
     1321 cgtcgaagta atggaagtaa acaaaatgat tggttttaaa tttgggatcg tcactttgat
     1381 aaactgctgc catttagaag caccgtcaat agatgctgct tcataaatct ccgaatcaat
     1441 attttgcatg cctgaaagga aaaaaatcat attgtatcca gtccaacgcc aagtgatcga
     1501 aagaatgatc agaactctcg cccaaattgg atgagtcaac cagccaatcg gtgtgtcaat
     1561 cataccaatg cccaacaaca agttgtttac aaggccagat tgagaaaata gacttttcat
     1621 gatcagagaa taactgacca tcgaagtgat acatggaaga aagatagcgg ttcgaaacaa
     1681 ccctttatat ttcaactttt tatcatttaa aaggttggaa ataaaaagtg ctagaaacaa
     1741 catgatcggt acttgtacaa gtaaatacag gaatgtatta aaaaaagctt ttttaaaagt
     1801 tgcatctgtc aacatccgct gataattacc taacccgtta aatgtcatat tgacaggggg
     1861 accactttga aaagacgtga tcaaagccaa aaccatcgga ataaaaacca tgattgatat
     1921 caaaatgact ggcaagatta aaaaagcatg cccgtttttg aataaatctt tctgcttcat
     1981 tcttttccct ccaattctat cattgattat ctacatgaaa agacaagaaa gacaatatct
     2041 gtaaggcaga cgtgatcttt tttgtctttt cgtttgtctg atgaaaaagc gaagaaattc
     2101 acttttttac caccgatcaa ttagccaatt gtgcttctac ttgtttttgt gtatctgcta
     2161 gtaccttgtc tgtctcttct ccattgatga ttcgatgaag agcttcagct acgactgctt
     2221 caattgcata ggtctcatgt ccgtaattca cttctggtat ttcagatgtc catgttgaaa
     2281 aatcgtcaaa gatcttttga ccactgtaaa attcagaagc ttcttgataa gcttcttgtt
     2341 cattcgcgct aagcatcgta gatactagtc cgatttcttt cgcaagagtg cccatcagtt
     2401 cttcattcga agcaaacgtt gcttctagga aatctttcgc tgaatcttct ccagctactc
     2461 ctttgatcac ataccagcca gcgccgccaa ggttagaagc ttgcgctttt tgcagatttt
     2521 ctggaagtgc aggtattggt gcaatcttcc attttccaga ttggttttct gctccttgga
     2581 tcgttgaaga ataccaagcg ccagttgggc tagaagctac tgctcctgat tgtatcgcag
     2641 ttactccagc attccagtca gaggtttgtt cgactagatc ttctttcaaa agagtggcaa
     2701 acaattcaag accatacttt aaagatgcat tgtcttggat cgtgactgtc tccccatctt
     2761 cagatgtata ccattctcct gcctgttgca taatcatcct tactcgacca aggtcagaag
     2821 gattgacttc agcgattttc tttcccgtct tttctttgac atctcgcgct acttgaatat
     2881 actcgtccca cttcagattc tccatatcct cttctgtata acctgcttct gccatcagat
     2941 ccgtacgata gaagtttgct gttacgcccg aatcgaaagg aacaccgtat attttatctc
     3001 ctactttgtt taccgcaaat ttatacggtg caaaatgatc ttcctttaca atagcagaca
     3061 aattagcgaa tgcatctgga taagaagtca aatagccttg tatcctataa tcttcaatca
     3121 agacgatatt gggcaatcct tctgtattcc cgctcgctaa agcggtgttc agtttctgaa
     3181 cgatatcatc ctgagacatc gtaacaactt ctatctctgt ttcttcgttc tcataaactt
     3241 tttttgcttc attcactgct ttgatattga atgtctcatc ccacgcccag acagtgactt
     3301 tttcttcttt gtctgctgta ctactagcac ttccgcatcc tgttaagaaa cctgatgctg
     3361 ccgctaaaca aacgactcca gcaattaact ttctgatgtt catttttatc cgctcctttt
     3421 ccatccatta gttgtttacg tttgctacat tcatcatacc aaaatttgaa aacggtttct
     3481 ttattattca gatacttcaa aaatttctcc taaaataata aaaaaataca aatcaactat
     3541 gacaaaagga ataatcttag catctagacc aaagatcgaa atggtgaggt ttctttaaaa
     3601 taaaaatatc cttgcatttt ctcctagaaa taagtatttt taaattacaa gactattctt
     3661 aaggcgggta aaaaaatgcc aatacgcgta attcttatcg atgatgaaca gacgatttta
     3721 gatggaatga aacagttgat caattggtct gagcacgggt ttcaattaat agggactttt
     3781 tccagtccgc tgaaagcgct tgatttttct ttggtacatg caatcgatat cgtggtcaca
     3841 gatgtaatga tgcctgaatt aaatggaatc gaattatcgc ggctattaaa acagcaacaa
     3901 cctgaaacac agatccttat cctctctagc tatgatgaat ttgatatggt taaagattct
     3961 tttaaagaag gcgtcgctga ttatctttta aagcctcgat tgagtcctga tttttttcta
     4021 aattccttaa ctgccgtttc acaaaaaatc aaaaaaaagc ccaacaaaag cccaatcacc
     4081 aatcgttatg agcagatctg cgagaatctt tcacagcaga ttgctggaca aactcgtcca
     4141 ttgcttgacc ctcttctttt tgaacacacc ttgtttttct gttatatacc gaaaaacggc
     4201 cattcagttc aaaacaaggc tctaaacaaa aacagtcgat cacccattca ttgatggata
     4261 gcgacgattc gtttactatt tatcctgttt ctaccttttc ggaagaatcc ggctatgtaa
     4321 tcaatactga ttccaaagat acacttagga agtttgttca aaagtttcgt caagaagttt
     4381 ttccaggtga tttttttgtt gtttctgatt cttttgcaat ccaagatatc aatgaaatat
     4441 tccaagagat cagaaattgc accaaaggac aacgtttcta tcataaggga aagaaccttg
     4501 cttatcagca tgaacttctt ccgttattaa ccaaccacga gcagcaagca agtagttatt
     4561 taacaaaaat cgttcataaa gactacgctt tagctgtcaa agaacttgag aatttcttca
     4621 cgacgatatt ggaacagcct attgatccag cttatttgaa acatgaggca atcaatcgtt
     4681 tctatgcatt actcaacgct ttggctgacg agcatgttgg gaatgaagaa ctttctttgc
     4741 tcaagatcgc ccttccttct ttattagccg atgctgagta tttcgaagat ttttctattc
     4801 ttgtcaaaga aacacttgag aaactcaccc acttttctgc aaatgcaaaa aaagacaatt
     4861 tcgatatctt gactttgatc tacgagttcg tccaggaaaa ttatgagaaa gaaatcaatt
     4921 tacaactttt atccgaaaaa tatcattttt catatagcta tctctcagcc atctttactg
     4981 aaaaatttgg tatcaacttt tcaaaatatc taaaaaaagt ccgtatcagt aaagccaagc
     5041 tctttttgac ggaaacacgg cttactttga cggaaatctg tgagaaagtc ggctataccg
     5101 aactgggtta tttctccaga gtttttaaag aagagacagg aatgacacct tctcaatacc
     5161 gtaaaggaac ttttgtaaaa tgagaaaaaa aatcaaagag catgaactct ttgccaaact
     5221 tctgttcatt ttgttcatcg gtctttttct gcaagccatc gtcatttctt tgtttattta
     5281 tcatcgatca agggatgcct atatccagct gttcaatcaa tcgaatgatg tggtactaaa
     5341 aaagattcaa tctgaatttg aaagtttgaa tgatacaatc gagaatacat tagccgcttt
     5401 tgattctaat ccagctgtca aaagctattt ttccaatgat ccagcccaac atatggaaca
     5461 gcttcagcaa ctaagaacaa tccaaaaaat gaatgactct ctttcaaaaa tccatccaat
     5521 gatcgattac gatgtattga tctttggaga aaatggaaga acttttgtcg gaaacgacat
     5581 gctcactgcc gtatctgccg attctttttt tcaatctgcg attgctcaga gagtgaatga
     5641 acgtgctgct gatacacaaa tgctttttgc acaccacgga ttgactcttc gcgataagaa
     5701 agcacccagc gtcttctttg tcaaaaaact gaaaaataca ttgaatcacg tttacggata
     5761 tgctgtttta tcgatctcat ccaagcaact agcgaatctt tttcaatctg tagtaaatcc
     5821 tgagatatcg aagatctcct tcatgaacga agaacagacg atcatcgcat caaatgaaga
     5881 aaatacaatt gggagccgat caatgatttt caaaaaacta acagtaggtg aaacaacgat
     5941 ttcaaatgga cagcagttga cccgtcttcc tctgtaccgc caggattgta cattactcag
     6001 tcaagtcgat cttcattctt tatctaacca aatgggcgtg atttttccga ttattttgta
     6061 taatatcgtg acacttgctt ttgctggctc attggtattt atgtatttgg atcgacacac
     6121 aaaatcgatc taccgcttga ttgattcgct aaaacggcta gaggaagatg gcttaaaagc
     6181 tactgttcct gtacaaggaa gccatgaagt ccaattgcta gggaatacga tcaatcaatt
     6241 gctaagtaat gctcagcatc attatgaaca gacgatccaa gacgaaaaga agaaacgttc
     6301 gcttgaaatc caaactatgc aagcacagat acagccccat tttatttata atacgctgac
     6361 tagcttgaaa tttttgattt ggcagaaaaa aacagatgaa gcagttcaag gaatcgacag
     6421 tttcattact ttactaagaa gtacgattgg gaaaaaagaa gaagtgattc cagtcaagga
     6481 agaaatcaaa agcgtacaaa gttacatcga tcttttgagt atgcgatatg gcgaaagaat
     6541 ctcggctaaa ataatgattc ctgatgaact gcagtcatta tctatgccga atatgatcct
     6601 tcaaccaatt atcgaaaacg cctatcttca tgcttttcac aataaaacgc aaggattcat
     6661 cacggtgtac ggcaaaacaa aaaatcagct tcttttattc gaagtgattg ataacggcga
     6721 tggcttcgat actgaaaata ccaaacaaaa aaatgatcat ttttcaggaa tcggcatcag
     6781 ccatgtagat gagcggatac gcctaatgta tggcgatgca tatggcttaa cgatccaatc
     6841 agtgattggg acaggaacga ctgtaacgat ttgtctaccg cttcttccta aaaatcaatc
     6901 agaatatcaa aaataaagaa tagccaagcg ctgatttttt tattttaagt taaataaatc
     6961 aatcagtatt cttgactttc atctacgcgt ctcttctact gtcagcactg gataacagcc
     7021 caatatttga ctttctcctc cgagcatctt gatttcttgg agcgcattct ctactaatgt
     7081 cattggacgg tccaacaaca aatcgatcac gaaaaaatat tctcccaagc tagttttcaa
     7141 tggtcgggat tcaatcttac ttaagttgat ttcccgccag ccaaaagctg ccagcatttt
     7201 atgaagcatc cctggccgat tagcaggtag tgtcaagatg acactcattt ttactggagc
     7261 tgactgctgg ctggtcatct ttcggtcacc gacgatccaa aaacgtgtct ggttcaactc
     7321 attatcttgg atattctcag ctaagatctc caatccaaca tgctgtgctg tttctaatga
     7381 agcaatcgct gctgcgtctt ctttcggatg ttctgctaca tacattgctg cagcagttgt
     7441 tgattctgtt gctactaaag gaacattagg ataatgcgtt tctaaaaact gttgtgattg
     7501 tgctagtgct tgtggatgtg aaaggatttt tgtaatcttt gttgctggat tgcctaaaag
     7561 ctgctgcttg ataggaagaa cgatttcact tttaacttcg acttctggat gtttggaaag
     7621 aagatcgatt gtgtgatgaa cagctccttc caatgaattc tctacaggaa cgacagccag
     7681 atcgacttgt ttgcgaaaca aagcgcgcaa acaaacaggg atggaagcat acgcagtcaa
     7741 ttgttctgta ggaaatagct gacaagccgc ctgataagtg aatgaacttt ctggccctag
     7801 atagctgacg ttcatatgct tttgatcctt tctataatgg tatcgatgat cttttctggt
     7861 gtttgattgg tcgtgttgat tcgatgtgtc gcactttctt cataccaaga aagtcgttgt
     7921 gccaataatg cttttatttc ttctcttgtt tttccaagag ctaaaggtcg aatagacgtt
     7981 tcatcttgtt ggatacgatc aactagacag tccgcttctg cctctaagta aagaacaatc
     8041 ggctgtgttt ttaaaaaacg ccgattttct gcactttgga caatccctcc accagtcgcg
     8101 ataattttgt cttcatttga cagctttctt aaaatatctg actccacttt tcgaaagctt
     8161 ttttcaccgt aatagtgaaa atattcggca atcgacaatt ggatatgttc ctcaatcttt
     8221 gtatctaaat ctacatactc tttttgtaaa cattgagcta accctttacc gattgttgtt
     8281 tttccagcgc ccatgaaacc aatcagcaaa atgctagcca tgagattctc ctcctgacac
     8341 aagttctgct aaatcgtcaa aaaatgccgg ataagagatt gaaacagctt cagctccttc
     8401 tagttcgcaa ggtccatcag ctatcaaagc agctacttgc agcatcatac cgattcgatg
     8461 atcgcctctg ctggacactt ttgcgccatg cagaggtgtc ggcccatgga tgatcagacc
     8521 gtctgctgtc gcttttatct ttgctcccat tttctgtaat tcttctgcca ccgcatcaat
     8581 tctgttggtt tcttttactt ttaattcttc tgcgtctttg atgatcgtga ttccttctgc
     8641 ttgagtagcc actaaagcta gaatgggcaa ttcatcaatc agacgcggaa tgatttctcc
     8701 accaatcgtt gctttcttca aatgactcgt cttgacactt aaatcagccg attggttctg
     8761 ttcattgtga ttcgtctggg taatcgctgc gcccatctcc tccaacacat ctaagatacc
     8821 agttcttgtt ggattgaccc cgacattttt taacaacagt tggctttccg gaacaagaag
     8881 tcctgctgct agaaagaaag cagctgatga aatatcacct ggaacagtta tctgttgacc
     8941 agttaatttt tgcggtccgg tcactatgat cgttttatct tccactgtga tccttccgcc
     9001 aaattgacga atcatttctt ccgtatggtt acgggaacgt tctttttcaa cgattacgga
     9061 tgtgccttct gcttgtaatg ccgcaaacag caaagcagat ttgacttgcg cactggctac
     9121 aggcatattg tatcggatag gtgataaaga gcgggttcct gtcacagtga ttggtagata
     9181 ctggtcattt tcttctcctt gcaagtcagc gcccatctcg cgtaaaggtt ccatcactcg
     9241 ccccattgga cgtttagaca acgaagcatc cccgaacaga gtagtcgtaa aaggctgtcc
     9301 agccaaaata cccatcaata aacgtatggt cgtaccggag tttcccatgt caattgattc
     9361 ggttggttgg acaaatgagt ggctaccgcg accatgaatc ttgatcacct cttcctcttc
     9421 ttcgatctcg acacctaatt gttggaaggc atgtaaagta ctcagacaat cttctgcgcg
     9481 caaaaaattt tgtatagtcg tcgttccttc agcaattgct ccaaacatga tgctgcgatg
     9541 ggaaattgat ttatctgctg gtatcctaac agtccctctt aatccatgta tttgttgtaa
     9601 taattgcatt tatccagcct cctatttgat ccagcaatga taactggtat tagacataat
     9661 gattcttttt gcttgtaaca aatcctgttg gtttttaaaa ctgatttgca gtattcctaa
     9721 aatatcttcc ctggtctctt ggatcttcaa attaatcaaa gaaactcccg cttttccgat
     9781 cagtccagtt acttctgcaa taacgccagg ttcatctggc acatcgataa ataaatcaaa
     9841 aaaggcagga atcgcgcctt gcttatggac aggcaactgg tctcgacttt cttttgcccg
     9901 attgaaaaaa gaaaagattt ccgattggtt ctcagtcatg atccattctg ctatttgtgt
     9961 catttcttgt tgccaatctg ccaatcgttc caataatatc tgcttattgc tcaacaaaat
    10021 atctgtccac atgacaggat ctgaggaagc gatcctagtg atatctcgaa agcctcctgc
    10081 tgccagttgt ttggctcgcg gatactcttg attgaattgt tctgcctgat tgactaagcc
    10141 agctgccaga atatgtggaa aatgacttag cattcctgtt atacggtcat gttctcttgc
    10201 tgtaagagtt acatattttg ctttggttcc gcaaaatagt tcttgcagtt tttcaacatc
    10261 ttttttcgtc ttttccaaag gtgtaaagat gtaataggca ttttcaaata gtttagcatc
    10321 tgctgcctgt atacctgatt tatgagaacc agccattgga tgtccaccga caaaccgaag
    10381 atttttctgc tcagcggatg tcacgacttg ctgcttcgta ctgctgacat ccgtaatgat
    10441 gacgtgatca gacaagttga ttctttcaag gacatcaaga taagctaagg ttgtacggac
    10501 gggaacggct aataagataa cgtctgcttg ttctgcacct gtttcaaaat cacttgcaac
    10561 gtaatcaacg atctgctgtt ctactgcttg ctcacgggtt tttgactgac tatcccaacc
    10621 aactactatc agttctggat tggtctgacg aatacaaagt gccatagaag caccaatcag
    10681 tccaagcccc acgataaaaa catgttgtgc catcttattt gccccctttt tagtaatgtt
    10741 tcaagtagtc acggtactcc ttcactgcag ctttcaactc aacaagcgaa tcacttggga
    10801 attttgacaa tagctctgtc gctaccacag tggctacaac attttccgca acgacgctag
    10861 ctgcaggaac tgctgtacta tccgaacgtt ctacacttgc tttgtaaggt tcttttgtat
    10921 cgatatcaac actttgaagt ggcttgtaca atgtaggaat gggtttcatg accccgcgaa
    10981 tgacaagtgg ttcgccattg gtcattccgc cttcgaagcc gcctagatta ttggtacgtc
    11041 ggaaatagcc tctttcttct tcccatagga tctcatccat cacttctgag cctggtaatg
    11101 aaccagaagt gaatccgacc ccaaattcta cgcctttaaa ggcattgata ctaaccacag
    11161 cttgtgcgat tttggcatct agcttcgtgt cccattgtac gtagcttcct aatccagctg
    11221 gaacgcctcc aacgagcact tctacaattc ctccgatcgt atctccattt ttctttgtct
    11281 gatcgatcaa atcttttatt ctttgctcaa ctgctggatc aacaactcgt acttctgacg
    11341 cttcagaaat ttctttgatt tcttgaatcg agatatttcc aggtatttct gcttggattc
    11401 caccaagaca aatcacgtgt cctgctacga tgatgtcaag ttcttccaga attttcttgg
    11461 caattgcgcc gattgctaca cgcatggtcg tctctcttgc agaggaccgt tcaagcacat
    11521 tccgaagatc tcgatgatgg tatttcatac cgcctaccaa atccgcgtgt cctggacgcg
    11581 gacgtgcaac tcgccgcagt gattggtctt tttcaggcac ttctgctaca gacatgacag
    11641 ttgtccaatt tttccaatct ttattttcta ccactaaagt gacaggtgaa ccaattgttt
    11701 tgccgtgacg gatgccagac gtgatccgaa cacgatcttt ttcgatcttc atccttcctc
    11761 ctctgccgta accagcttgt ctgcgagcga gttcgtgatt gatttcttct tctgacagag
    11821 gtaatcctgc tggtagtcct tcaataatgg ctgttaattc tggtccatgg gattctcccg
    11881 cagtcatata acgcatctta cagtcctcct aagtaatctt tgaatgtctc tgtgggaaca
    11941 ggaacgattt ttgcttgccc aatcttttct agcaagatga tattcaattg gcttcctctt
    12001 gctttcttat catgggttat cgcgtggaat aattcatctg gatgacttag atcgtacgta
    12061 atcggcagat ggaatttttg aagtatcctt tctaaagcag ctgtcgatcc agttggagac
    12121 aagccgattt tttctgcatg agaagtgatg gctaccattc caatagctac tccttctcca
    12181 tgactgacta ccccatatcc tgctgtattt tcaattgcat ggccaattgt atgaccaaaa
    12241 tttaaaataa gtcgatttcc ttggtcaaag acatcttctt ccactacttt tcgtttgatt
    12301 tccaacgtag cagtaatgat tggaacagca tgttccagaa gatctttttc gtctttcagc
    12361 tcgttcagct gtttccaaag cgttgcatct gctatagctg ctgatttgat gatttcagcg
    12421 attccttctc gtaaacgacg taccggcaaa gtttttagaa cctcaggatc aatcaataca
    12481 ccagctggct ggctaaatgt tccgacaagg tttttcgctt tgtttgtatt gactgctgtc
    12541 ttcccgccaa tactagaatc tacttgtgca agtagcgtag tcggaatttg aacgaaatgg
    12601 atcccacgca tgaacgtcga ggccacgaat gctgctagat cacccaccat tcctccacca
    12661 agtgcaagta tcccatcgct gcgggtaaaa tcattatctg ctaaaaaatc gtataactca
    12721 gctgcttgtt ctaaagattt actggtctca cccgccggta cgattgaaag gactggttca
    12781 aaattagctt gtctcaattg gaacatgatt ttttctgcat atagtggagc aacgtttgta
    12841 tcagtgatca cagctacttt ttgaggtctc caaatcgttt gaagccatga tccagcctgt
    12901 tctagtgcgc ctttttcaat atgaatatcg tatttttggt tatcaacatg tacagtaagc
    12961 attcgtttca cttctttctt ttaaaaagct ggaattaatc gagtgtctga atcttttcca
    13021 ttgcttcttt catgatcgca acttctttca tcatgcgtga gtaagtcttt tcattcaacg
    13081 attggggacc gtctgaccaa gcatttgcag gatcaggatg gatctctacg atcatgccat
    13141 ctgctccgct tgccacaccc gcacgagcca ttggtggtac aagttcccaa attcctgtac
    13201 catgacttgg atcaacaatc acagggaaat gagttaattt tttcatcaat gggacagcac
    13261 ttaaatcaaa cgtattacgt gtcgctgttt cgtaagtccg aatcccacgt tcgataaaaa
    13321 tgacaggaga tttgtcttcg actgcaatgt attctgcagc attaagccat tcattgatcg
    13381 taccggaaat tccacgtttc aatccaacag gtttacctgt tttaccgaca gcagatagca
    13441 gtttaaaatt ttgcatatta cgtgcgccaa tctgtaggat atcgctgtac tcagccacca
    13501 tatcgatatg cccttcatcc atcacttcag taatcacttt cattccaaat tcatcagccg
    13561 cttgacggat gtatttcaat ccttcttctt ctaaaccttg gaaagcatat ggagaagtac
    13621 gtggtttgaa ggcaccacct cgcagaattt ttgcgccccc ggctttggcc attcgcgcac
    13681 attctctgat ttggtctaat ccttcgatag aacaaggacc agccatcgtc acgaaactgc
    13741 cgtcaccaat ttttactccg tctacatcca cgactgtatt gccaggatgg aattctcgag
    13801 aagtcaattt gtatgtgttt aaaatacgca cagcattttc tactccttca tagcgtgtaa
    13861 atgctccttc ttgtaaactt cgcgtatcac cttttagtcc aatcactact cgttctgttc
    13921 cttgatcgat tgagactttg aatccttctt tttcgatatg atcgacgact ttttttattt
    13981 gttcttccgt tgcttctttt ttcataatta ctatcattgt ttttcccgct ttcttttgtt
    14041 tacttatcta tcttggctga ttttctctac tttttcaagg tggttttaca ttaattccta
    14101 ctaaatgacc gtgtcctgtt gttctcattt tcgctccttc tttcctactt atgacccaga
    14161 taatcaacaa aaaaagatgt ccctgtagga aattgcctct acagtgaaca tcttcaacga
    14221 gccttaaaaa gcgctgcaaa aatataaaat agccactata gatgaatttc tctacgtggc
    14281 tgtaagtcgt gtttaaaaat ctttagccat catagataca aacctggttt gtttgtaccc
    14341 acgaagtcat gaatacaaac ctatctaaaa tagctaaagt aatacgtatt cagttttgta
    14401 agttttctag atttcataaa aaatcgctcc aaactgatgt aagaattgta cctagtatag
    14461 acataaaaaa atcgactgtc aatcattttt atgaaacttt ttttaatttt ctgatagtta
    14521 actttgtttt tcagaaaaat aacaattggt tttattgaag aaaatagtca tgaacaaaag
    14581 tatctgagaa cttttttact ctcttgttca tgacatatag aataaagcag ctttgtcttg
    14641 taaactccgt ttttcaaata ataatctaaa gattgctact gaataataaa gccgatttca
    14701 tggtcctctt cgctgctgat tcggtaaggt gactcaacat ccgctcccca gctattgata
    14761 cccccgactc ctcttacttt tgcaaaaatc gtaagaaccg ttcgtctttc tggcggtagt
    14821 tcttctatat gtgttgcctg ttccagttct tctgctttga atggtaaagc ggaaaaagca
    14881 aatggcgtat cctgctgttc aaatcgcaac gaaaaatctt cttgatcttt atccgtatta
    14941 tccaacgttt gatttcttgt tagttctacc catttcgttt ccatatgcat gccgcattct
    15001 tggggaacaa ggtatgggga aacggggagt cctgccactt tataaatacc ttcgacacca
    15061 ccagccatcc gatccggata tgtttcccct gataggcctt catactcaaa aaattttgct
    15121 ttcgttggca taatgaacct taaacccaat gcgggtaatt ccggtaatcc tttttgtcca
    15181 taataatgca tattgacaag cattttaccc tctccagcga tttggtagga aattgtcact
    15241 tgggtttttg gtactgtgtt gatttccagt gtaaaaatga tttcaaccgt ttcagcgtat
    15301 tcattacttg aatattgatt atttactgga gcaatcggca aatcgatctt tttattgtcc
    15361 accagtactt ctatttcttt gacttttgga tacaatccgg ctcctaacca cataccggat
    15421 ttaaatccaa atccgctacc tctgtcatta tcggtcgttg ctcgccaaaa aactggaagt
    15481 gtttcccggt atagccattc ttttccgttt ttattgagcg attccagtcc gcctcttgta
    15541 taagaaaaaa tataatgcgt attccccgat ttgacaccta atgtacagtc gccgaagacg
    15601 attgcaactt tttccgtatc catatgactt ttatttactg tatttttcat aataatatct
    15661 tacctcctgc atggcgggtt tttcttgtcc atctgcaaaa acgatcccat tccctgagaa
    15721 ttcatagtca cttggtttat cgtcaaagtc accgccatag cgcatcactt ctctgcctgt
    15781 gattggatca gtcacttgga tcgcctgatc tttaaaatcc catatatagc ctccttggta
    15841 catttcataa cgatcaagca acgtcatgta agaatccatt ccacctaacg aattccccat
    15901 gtcatgcata tattcacata agatgaacgg tttttccgct ttattttcaa ggtagtcaat
    15961 gatctcttct ggtggcgcat acatgcagct ttttacatct gaaatcgaat cttcgaatgc
    16021 tggatttgga aaattccctt cataatggac aagtcgatcg gggtctactt ttttgaagta
    16081 atcgttcatt tgtcgaatgt tttcttccgc ataagattca tttccaagag accaaaaaag
    16141 aatacttgga tggtttttta atgtttcgaa attcgttttt gctcgatcaa gaacagccgc
    16201 ctgccactga ggtactgcac ctggtacgtt gtaagaaggc tcaaatgcgc ccattttttg
    16261 ccatgagcca tgggattcga ggttcgtttc cgccatcaca tggattcctg cttggtcgca
    16321 taaataatac caagtgatct ggttggggta atgagatgtc cgtacgccgt tgatctttgc
    16381 attttttatg atagccaaat ctttttccat atcttctata gtgatggcgc gtcctttttt
    16441 cggatgccac tcatgtcgat ttaccccgtt gataatcatc cgttttccat tgaagtagat
    16501 gattttatct ttgatttcga ttttacgaaa accgaaggaa tagtcaatat actccgttac
    16561 tcttttttct tcgtctatcg tctccaattc aagcttatat aaattgggct gaaaatgccc
    16621 ccacagtgcc acattttcaa atccatcgat gcgaatcgtc atttgctctt ttaacggata
    16681 ttgctgttga tagatttcct tcccatcagg atctagtatc gctacattca gctgactgtt
    16741 tttggctact tgctcaaaag acaaatctac ttcaagtttg ccattttgac atttgtcatc
    16801 taagcgaggt ttgatccaaa gatcttctaa atggttttgt cctcgagcaa ctaagaagac
    16861 atctctgaag atcccaaaaa atcggaaaaa gtcctgatct tccaagaatg cagctgtact
    16921 tcttttgtaa acctccacag ctagaatatt ttcttcttcc aaataaggtg ttaaatcaaa
    16981 ttcagacgga gtgaacgaat cttcggcata cccgataaaa tgaccattca accaaataaa
    17041 cattgcttgt tctacaccag caaaatgaat cgagatttgt tttcccttta gaccttcttc
    17101 taatcgaaaa gtcgtcaaat acgaaccaac cggattgtct ttagcagctg aaaagatccc
    17161 ttccgcctct ttctcagggc ttgtcgtgaa agggggacgg cgaaatacat gtccctccca
    17221 cggatacatc gtattgatgt aatggatctt gtcataattt gctaattcaa tatgactagg
    17281 aacagtgatt tcatcaaaag aacaggcatc aaaatctttt ttataaaatg tttttggtcg
    17341 taccgaagga tttggcgaat aacaaaaacg ccatgatcca tttaaagact gtttaaggga
    17401 ggatgtggtt tctacttctt cccttgttgc ataacaaaca tgatcgctat gtgcatttga
    17461 ttggttcact ctaaaaactt gtggatcatc taaccatttt atatttgctt tcatactttt
    17521 ttccttctct ctcataactt tttgaaatga agcagttctg tttggaaatc accttgaaaa
    17581 tcggggaagt aaagtccaac tgtcatcaat tcggcccccg aaaatatccg cttcgaggtc
    17641 tgttcctgat aaagtgcctc atcatctaac cccatcagat atacttcatg gaacgctggt
    17701 tgagctgaag ctagtatcct ccctaaaaac acaatagctt catcttgttt aggagacaca
    17761 aacatccatg cggcaatatt ttcttcaaaa ggactcgcca aacgataata atggccatac
    17821 tggatcaccg gacgaatcgt ttgataggat ataatttggt cttttacttg ctttttttct
    17881 tcaggagaca gctttgtcaa atccagttca taaccaaaaa cagcgctcat agctgtttct
    17941 cctcttgttt tcaacgaagt agatctgtcg gtctgatgat ttggtacagc tgatacatga
    18001 gctgtgatgg acgaaatcgg ataagcaagg cttgttccat attggatttt catacgctcc
    18061 actgcatccg tgttatcact tgtccatgac tgcggcatat aataaataaa cccggcatca
    18121 aaccgtccac ctccaccaga acagccttcc cataagattt ccggataatc agttgtcaaa
    18181 tgttcaagta gttcataaag cccgagcata taacgatgcg ctacttctcc ttgtctctgt
    18241 gggtcaaggg taatcgaata gacatctgaa agcgaacggt tcatatccca tttgatgtag
    18301 tcaagaggaa tcgtatctaa aatctttctc atttgtttct caatcgtctg ccgaacatct
    18361 aaccttgtaa aatccagtac atgttgtgat ctggaagcag aaggcattct acctggctcc
    18421 tgcataagaa aatcaggatg tgttcgatac agttcagaat caaccgagat catttcaggt
    18481 tcaaaccata aaccaaattt caatcctttg ctatgagcat aatctgctat ttcacgtaat
    18541 ccatttgtca acttgccttc atactcgaac cagtctccta atgaagaatt gtccgcatct
    18601 ctttttccaa accagccatc atctaatacg aacatttcga tgcctagctc ctttgcttca
    18661 tcaatgatcg ccttgatttt ttcactattg aagtcaaagt aagtggcttc ccagttattg
    18721 accagaatcg gccgttcagc aaattgatga cgacctcttg cgattctttc acgtagcaac
    18781 tcatggtgga tctgactcat tttgttcaat ccctgacttg agtaagaaag gatcatttca
    18841 ggtgtttgaa aagactggcc tgcattcaat tcccacgaaa aacgatggct gttgatccca
    18901 ccgacgactc tcaattgatt gatctgatct ttttcaagtt cgaaagaatg gttgccggaa
    18961 taaacaaatt gaagtccgat cgcttctcca gtattttctg tcgtatgatg atgaactaaa
    19021 gcaataaagt tattcatatg gtgactgctc gttcctcgac ggctttcaaa tacttttgtt
    19081 ccagataaaa cagattgccg tgaaatctgt ctttcattga catgtgctcc tggcaaagca
    19141 atgacttcat caaatcgcct agtatgggca aagtctagct ggaaactagc cgctttttct
    19201 agtttgattg tttctccggt ttcatttctt atctttacag atcgtgtgat cactgctcta
    19261 tcagtaaata tggtatagaa caaatcgaaa taaatcgctg tttctctatc cttaagtgaa
    19321 atcaccaacg tttgtgcctc tgacttgtcc atcacataag tttgaggcaa gccttcgatc
    19381 tctggcttcc catctgtaat ataatggctg tcatatctca gatccagtaa gtctgatcca
    19441 ttcttgcgct gaatgatcga tgcgggcgtt cgaaagtcca tgctgccatg agaagagtat
    19501 tcttgcggta gtgtatcttt cgacagagac cggtcaggat ttaagggaac atttcctgaa
    19561 aatccacgat ctcttcgggg ataattttta ttgtctttgt actgcttgac tggtttgcca
    19621 aaataaatat gcgccaatac attgctttct tctatcttta tcagataaga aagaaatgta
    19681 tttcgtaaat ggaagactcg gctcttttca tcaaataaga tgaaattttt atctgttttt
    19741 ctatgcgtaa tatccatcat ttcactccca acaaaacgga aaccgtttac ttttttttat
    19801 aattcctgta tactgtttac taaacaattt tgtcaataaa aattttttat ttttttgagg
    19861 agtgtgtaaa aatggttgga attagagatg tcgcacgaaa ggcaaatgtc tctcctgcta
    19921 cagtttcccg tgtgttgaat aacgatcaga cgatccatgt ggctcagact actcggcaac
    19981 ggatagtaga ggcagctgct gcgttggatt atgacatcag tactcgtaaa tatatcaaaa
    20041 agagaatgcc ttcgattggt gtgatctcta cgatcggcaa agcgagcgaa gaacaggata
    20101 tctattacaa acatttgcga gtagggatgg aagaagaagc tcgacgtctg catttaggga
    20161 tgaatcgaat ctataatcta tctgataatc ctaaacaatg ggaagatcta gatcagttag
    20221 gtgcggtgat cgttgttggg actgttacga aagaatcgat taccgccttg ttaaaacaaa
    20281 acatgcaaat agtcgtcgtg gataaccctg acatccagca agaggtcgat atggtgtatg
    20341 cagatcttga acggatgact aaaaccgtac tggaattgtt cttagaaaat ggtcataaaa
    20401 gaatagccta tatcggggga tataaagtcg atatggatga attcggtggg aagcagattt
    20461 ctataaatga aaagaggatg cgtgcctatc agcattttat gatcgatcat cacttagaaa
    20521 aatatatgtc ttatcatcta ggtggctgga cagaaaaaca aggtgaggta cttgcgacta
    20581 atctattaga aaatgaatcc cctcttccta ctgccattct tgtcgggagc gatccaatgg
    20641 caattggtgt ctatcgggca ttgaaacaaa aaaatatcca aattggagaa gagattgttg
    20701 tagcaagctt tgataatatc gagacaatcg caaacctctc ccctggcttg accagtgttc
    20761 aaatcaatgc gaaatcaatt gggcaagcag ctgttcgaat ggcattggag cgaattgacg
    20821 gtatacggga cgaaccaatc attttgactt atcctgcacg attgattgtt agggaaagtt
    20881 ttatcccaag aacaaaaagg tattgaaaga gaggttgtga aaagccgctt cgtttc
//
LOCUS       DASHHB010000049        20549 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.051, whole genome shotgun sequence.
ACCESSION   DASHHB010000049 DASHHB010000000
VERSION     DASHHB010000049.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20549)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20549)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20549
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.051"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..134
                     /locus_tag="U2K71_002048"
     CDS             <1..134
                     /locus_tag="U2K71_002048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEM7316081.1"
                     /translation="RYINGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            881..1111
                     /locus_tag="U2K71_002049"
     CDS             881..1111
                     /locus_tag="U2K71_002049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002370649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316082.1"
                     /translation="MRNYWYVSLTNEYPRTIDDCSVRVVRSVQIKGKYSIVEMGREAE
                     PCEIDACKLVYCGHGFYDEPNIQNNINKNLRD"
     gene            1270..2103
                     /locus_tag="U2K71_002050"
     CDS             1270..2103
                     /locus_tag="U2K71_002050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="small subunit of terminase"
                     /protein_id="HEM7316083.1"
                     /translation="MARQRDPRRDEAKRIWLESNGEKQLKEIASELNVSDSQVRKWKS
                     QDKWSAELISNVTNGKSNVTNQGGAPIGNQNAKGNKGNSRASPPVGNKNALKTGEYET
                     IFFDTLTDNEKDIYSSLNDDPSFALSEEIRLLKIRQLRMMKRIQQAEAGLNDEEVERL
                     QQLRKIKTPIEKNGKKLEIKREVMQDVQISRKKHRKIDDILSIEDSLTRISNQLAKAI
                     KQMNELYMNEYRTDLIKAQTDKIQAETNEIGGNNSGEEIEEWKQAVLNAANKRAVKEN
                     E"
     gene            2096..3511
                     /locus_tag="U2K71_002051"
     CDS             2096..3511
                     /locus_tag="U2K71_002051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEM7316084.1"
                     /translation="MNNEFIPFADIGSAIDYYYDKPVAFCQDILHLNPDEWQENVLND
                     LAEFSKVSVRSGQGVGKTALEAGAILWFLTCRPYAKVIATAPTMKQLYDVLWAEVAKW
                     LNDSLIKNLLKWTKTKIYMVGDSERWFATARTATKPESMQGFHEDHMLIVVDEASGVS
                     DPIMEAILGTLSGFDNKLLMCGNPNNIEGVFYDSHNSDRDKYRVHKVSSYDSKRTNKD
                     NIEMILKKYGKESDVARVRIFGEFPKGALDSFISLETVELATEKQISDSLVNKTKVAH
                     IGVDVARYGDDSTILFPRIATRALEYEKYSKRSTMETTGYVINMAKNLMSQYPSIDKV
                     MIKVDDTGVGGGVTDRLEELIEDKHYPFEVFGVNNGSTSEDDFYDNLGTQLWGNIKEM
                     LEENMTANLNGEQPVIELPSDSSLIKELSTRKFKMTSRSRIRLESKDDMKKRNIGSPD
                     IADALALAFYEPPSHYQFIQF"
     gene            3523..5052
                     /locus_tag="U2K71_002052"
     CDS             3523..5052
                     /locus_tag="U2K71_002052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM7316085.1"
                     /translation="MISTVLSLERYKKLRVKYATQIEDGMFDPNGFIEDMKPFFADRE
                     RKYLAYTSEKNEIDNRPKPNTDIVKVNNKLHAGMYSIVVDQAVNHFTGIPIKWDYDVS
                     EQKRTLIQRLKDKFLKNDIELPTVPEAFNKLTSNLDSMRFAMLDSETATFQGACGVAF
                     RLLEPVEEDDGWKLRASNIEPWRAERYGNAGIYIKEKYDSYQKKFFQEMKVMTRNKIL
                     TYACYGDLNFVTSGTFKKIDETDNPLGTITLAEFKNNTNRYCDFEVAEEIGDAIDRAL
                     SDQQNEIEQFKLAYMLVTGTTMSKGTAKEMMNQLGIINLKAPTAKAEYVTKNLAKDFN
                     EYHMDLLKKQFYTICKAIDFNDEVFKSNSSGEARKWQIISLEAKTNTKEQYFREGLKE
                     CAETIAAFLKFHDKVEIEPEKIIFTFSRSLPTDLSYLAEALPKLAPYVSKRTIQSQIP
                     FVTDVDYENEMMELESGSAYPDSEYNFGGGGNSDDRNEVLDQTSRTGGSSKAQTRKSD
                     T"
     gene            4970..6022
                     /locus_tag="U2K71_002053"
     CDS             4970..6022
                     /locus_tag="U2K71_002053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="minor capsid protein"
                     /protein_id="HEM7316086.1"
                     /translation="MTIETKYWTKRRELEDQARLKQENQTLKKLTSVFPEALKEIQAK
                     LLSQADLHNITYPEMMEFYSTSNQKKYREYVEKNYKSLKMYDAKYKEFIDEFFPPFDY
                     AKVNRLLQIRSDVFKILAEYAMDADVNQYFSDRLEEILQRTYSSNANVFVQLLNVDIP
                     NYLPENEIKHYLNYPWSGKTFSRRLWGNISSLEQKLSNAIVKSVASGEGVIHALNTMR
                     LDSEICDMFKLEESKYNKAIENLVRTEYAKFAQDGIEKSYLETGIEEYNVLTAKDERV
                     CRICGGKASKNPYKLKDAVIGENRAPFHSRCRCTDVPNLPKLGKDIDEEYDRLFGDLL
                     DEFAHDSFGINLKRRK"
     gene            6024..6341
                     /locus_tag="U2K71_002054"
     CDS             6024..6341
                     /locus_tag="U2K71_002054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316087.1"
                     /translation="MKDFFEAVLTINVNADIAEAYKTAIESENHPNGLRDHWNGNYAY
                     VVIGDQTVNYQDNTPVDKNTVNLTIQLLSHSLPNLKETVDWYEKMGCIVVRTDYKEGK
                     SSN"
     gene            6468..7091
                     /locus_tag="U2K71_002055"
     CDS             6468..7091
                     /locus_tag="U2K71_002055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4355 domain-containing protein"
                     /protein_id="HEM7316088.1"
                     /translation="MSMKHKSLMPMNLQYFAEGDDQKFSFDDFKSFVESNEEAQKFIQ
                     SQSQSVADKQLEAWKQNNLDKIKQDTIKEYEESKKNKSPEQIQLEKLQAEFEAEKALR
                     VTSDNKAFVAEQIAGLELDGELKESISQFMLNNLVSSDTDFTKNAVEGFTSVLNAINE
                     KHADALKELQMKSAFGGTQQSNNQVQQNNETLTNPEEQLGQILQQFN"
     gene            7106..7996
                     /locus_tag="U2K71_002056"
     CDS             7106..7996
                     /locus_tag="U2K71_002056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5309 domain-containing protein"
                     /protein_id="HEM7316089.1"
                     /translation="MKKTSLNNLEYLDISPAINAMQVPNTPFLSYLLGAGKTEPANST
                     EIKWREYDINNDDSSEKLEGGEYPDAESGRTWFNNYTEIFRKSTSVSGTLDAINVNGV
                     GNELTNQVALRGMEMKIDLNRKLITGVKADENGSKGRRMNGILNLINSANKAETATAG
                     AVTRKDIDTLFKLMYEKGYMGEKLCLISPDMQELMTDELDGKSTKIVQFGERVTFGLQ
                     IGNIVSNYGTGIALLEPSLPKGTIAAIDTNYVKLRPLREWRAEELAKTTDSRRIGLVG
                     EYSLEYNASNSGAILNLKSE"
     gene            8019..8234
                     /locus_tag="U2K71_002057"
     CDS             8019..8234
                     /locus_tag="U2K71_002057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316090.1"
                     /translation="MIGGITVVKKDETKKDEVVKYRVGKTKNFVGFVHPKTRRFITAD
                     SNNEFIISIDDKEAIAILEDAIDVNEI"
     gene            8246..8587
                     /locus_tag="U2K71_002058"
     CDS             8246..8587
                     /locus_tag="U2K71_002058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316091.1"
                     /translation="MDESLKTEIIESTKEDFPDLSEERITNLLEIILLEIESYNTCKN
                     DISWEKLKSVISEVLYKIIKNESEKTVSSVRRGDTTISYASTTNDVSELLLGYGDLIR
                     RVIGCGGLEFF"
     gene            8587..8958
                     /locus_tag="U2K71_002059"
     CDS             8587..8958
                     /locus_tag="U2K71_002059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316092.1"
                     /translation="MNEADILETTYEDSCIIERLTDIEDSNTNITIQDYKKVYDNPIS
                     CALSQGQIDGLAVIEDGEMVNVSTDTYKLFVHPKIKLKKGDRITITQKASGLIFSLFA
                     TKPFYYPSHCEVNLIGSEKNG"
     gene            8951..9346
                     /locus_tag="U2K71_002060"
     CDS             8951..9346
                     /locus_tag="U2K71_002060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HK97 gp10 family phage protein"
                     /protein_id="HEM7316093.1"
                     /translation="MGNLKYESNAEKIIENFKNMTVIAQKEGISFVNDSMNKVVSLAK
                     PLTPVKSGNLRRGYRVVKARKLSSGRIVGEVINNEHYFKYVEEGHRTKNGGFVKGKFM
                     LTRATNLANMSYIPRRFKQMAIKIVKKGK"
     gene            9348..9725
                     /locus_tag="U2K71_002061"
     CDS             9348..9725
                     /locus_tag="U2K71_002061"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316094.1"
                     /translation="MKDEIIAAISSKLKEIYPDGTIYLDSVMQSTKDFYFVLSVMESG
                     TENVGIDIQNVSFLIDIALIDNNPNRNLINELVSRCGTFFNTITIDEQILFPKAYLPD
                     EADGVQHIRFTLEFPQYIEWSER"
     gene            9726..10334
                     /locus_tag="U2K71_002062"
     CDS             9726..10334
                     /locus_tag="U2K71_002062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM7316095.1"
                     /translation="MGEKRSKTGIISVEKPTWFPLEDETGNFPVYGTALPMGTAVSIK
                     PTANYETTQDYGDSVVQDQFTAFGGAEVELEANGYTHKVLSTITGGKIVKGGALRSGE
                     DIAQDGAFAYRRKKSNGKYRYTVFYKGQFALDSDETSTIEGSKVSFTHPTWKGSFVDV
                     PGLGYMYSVDEDDEGVDKAMIGNWFTKVAIPIEEAELSGGTE"
     gene            10334..10675
                     /locus_tag="U2K71_002063"
     CDS             10334..10675
                     /locus_tag="U2K71_002063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316096.1"
                     /translation="MSKYQTTIKLTKKDEEGKYEQVQFKSAEFLPGTVVEDAAGVMEE
                     MQTATDKQSVKKALSRAYSFIADTLFEGQFTGEDYCKGIDAREIASLTGKLLKSVTAG
                     FDETYTETKKK"
     gene            10917..13706
                     /locus_tag="U2K71_002064"
     CDS             10917..13706
                     /locus_tag="U2K71_002064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tape measure protein"
                     /protein_id="HEM7316097.1"
                     /translation="MILDESGFTAGMNNAVKKLNSFDETIERKSRNSGSSLGSIWKIF
                     AGSFLASGVTRIVGAGFDLIKGSISGAVDRVDTMNNALRNFQNMGFSNSEIMKNIGKN
                     GLLSQGIQGLPTALNDAISHVQLLASSTGDLTRSTQIFKALNDGILGFGGSTDQVNEA
                     VIQLSQSFSNGKVDAQTWNSMINAQLGPTLSAIAKKMGITMGDLKEGLSQGKISVEEF
                     QNQLIEMDTKGGGGLKSLSQIAKDSTKGIKTSIQNAKTAVTRGVGEVIEGLNKALVDS
                     DLGGFKGIIDKVGSSMESFLKVIAANIPTAVSFLSNLFNEIQKFGSALKFMMPFLVPA
                     ATAFGAFMFQLKGIPAIIKSFNNFKNAIIGVGNSLKIMGAIAAANPFVLIIGAVVGAI
                     AVFGYFMATNEELRNKVISIWNDVKDSVIGVLKNIKDWGIDTWNSAKEMASNAVEGVK
                     DAWSGTKEWFSNTWKGIKDGATGLFDKTVETSRNAVDSVKNAWSNMKKWFSDTWQSIK
                     DSAREKWNGIKGSIMSVAGPLITGIKNAFLHVTFYLETLWNNLVEIGKNVFEILKNVI
                     LAPVLFITSMISGGWEETKNNMIAVWNNIKESAINIWESIKNIFVSYFTNIYFAALNI
                     WTGFKLTLINIWNEVVNQAKSIWLNIKYFFINLWIDIKYFAIQKWIELKFSIIQTWID
                     LKYNAITTWNNIKQFFKDTWKNIKDTAYNTWISIKNTMINTWNNIKDSFWNIVTGIVN
                     SAENAWTNLKNGVSNAINRVKEIFNSLREVNLFEIGKNIIDGLINGVKEKWNALKDTI
                     KGIAGNIKNWIKGALDIHSPSRWMRDMVGKNIVQGIIVGIDKEQSKLDQTMTNLVKTP
                     SVQPVVNGISHLPITQTKQNISASETKEIHLHLNVYGDLPDSMVRKITTKIKTELTRQ
                     MKRDAGAVGGTLYAT"
     gene            13696..14436
                     /locus_tag="U2K71_002065"
     CDS             13696..14436
                     /locus_tag="U2K71_002065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM7316098.1"
                     /translation="MQLKRGQFFINQHYSSEFNVYIQNRPASVSASRVIELREREGND
                     SIIIDKGYYKNVTRKIECYYKAPSIDMVQEWEDRITEWLDMSSYSDFILYYDEQYIYQ
                     AVVIEGPEFKGTRKTGNIVPFEFTVSIRPFKENYNGRFTIRQTETFEIYNPEKYSSKP
                     LIKLSGSGDASFYINKDKYDLKSLDRELFIDSKLEEAYRKLDGNLEHQDQVTLFLDFP
                     FLYPGKNEIKWTKNIHSFEIMPRWWRKV"
     gene            14433..17195
                     /locus_tag="U2K71_002066"
     CDS             14433..17195
                     /locus_tag="U2K71_002066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM7316099.1"
                     /translation="MKPRIYSPTETDFSTNGLGILKDCTRCGIYEVANGKYELELDYP
                     LGTRFDEYFENDYQIKAKPNDQEEYHIFFIDDKDIDTFLNTVTIYAQSRTNRLGRRVV
                     TLVEVDSKTGQEAISIIETKMDKKSDIRLYSDITAVSSTIFEARNVLNCIAGEQGSLL
                     QYWGGEIKREPFKLSLLKRRGRNNVGTIRYGKDMSGLKVKLDWTGIKTRIIPYADPQS
                     EVGTTNRIYGSPVDSAYINNYPDVYTEHVQFTEEQGVKDIKSLNKIAKNYFKTINPGC
                     DKPKVSITVEFDKLTDSEEAKEFAKIRNYGLFDTFKIYHKKYNIYLESKVSGLQYDSL
                     SEKTLKLEAGDIQVAFYQQQAVTIQDKLKDYATNNYMSDFNDYVSSMITGQGSAGGYV
                     VLWPKEKPSNIFIMDNPDLEKAREVLRMNKNGIAFSKKGWRGPFNSAWTLDSIFNANF
                     IQTGLIKADIFQNSFNKTGDVLKLVNGLLQIWNNKKKIMELTKKGMEFWNSKGSIGTI
                     GTTDSVGNPFPGASTPTPIEDNSLVIRTNGDGKYILISPKVGKGLVLLGNGKAIYFGD
                     LDVQGKLTVKGKEIIPGQGGGPSGGGESTGGYPSELKTDAEKRAWRIYDILCNNGFTK
                     QSACGILGNIQQETGGTFDPDTVQIGGPAYGLVQWDGSSYPLVGPATWDGKVYVQNLF
                     NAAGIKEPITSLDAQVRLLIWTFTNGQWMGVVQPTTVDGFKACTDPRQAAYAFERNYE
                     RPAATHPERQDYAVNWYNKFKDLKPGGATGEAGLKHLESLIGQRIGNGQCYGLSAEYS
                     GYLGGCGMGAGTKYGLTHVIGNTSAASDIGIAYDWSAVGWKVIQNPRYDQLVVGAIIN
                     WARGGQVGSWFADGTYGHTGVIRGLANGRMQTYEQNTELGMICGKLDRQYYSASAISS
                     IVIPPK"
     gene            17208..18116
                     /locus_tag="U2K71_002067"
     CDS             17208..18116
                     /locus_tag="U2K71_002067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage baseplate upper protein"
                     /protein_id="HEM7316100.1"
                     /translation="MAKWNVILSTTEPYNYVGMIQVRQGNKNTEVMEATIVENGLPYD
                     LSECKVYFESVVGGKYPVQLETKIVDAKKGKINYIFDKYSMQCLHRQTANFIIFKGED
                     LIGTTQDFSYFVINAVSKTEGEMGSYWQSIEDLIADMTDFINENKGDFTDWMNERKEE
                     FERWRQEQEHSFQDWREGQESDYLAWFESIKDILKTIDPGGVMLAELTDARVDIQGVR
                     HASISERLLADMDYLYQKLRATLFTIEYGEIEVTDILQDDLFSDNHEVEKVGTVAFPI
                     EEGALIIATVDDPKQNVFTLEKVGVI"
     gene            18116..18736
                     /locus_tag="U2K71_002068"
     CDS             18116..18736
                     /locus_tag="U2K71_002068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316101.1"
                     /translation="MAKTKRMMETDEKTGVQRQFFPITHASAVLGLEEIIAGEATVLS
                     VNGKIGAVVITKEDLGLENVLTELPYASEEDDGIITAEMYQKILNSGEGDYVLPVATI
                     DRLGGIKVGELLTIDETGKVSAVRQSDVNFSLELKEKLDSLKNYTAGENITIDEDGTI
                     SSTGGSGTGGVNQSYVDQKFQEAVNQAENYTNERIPNFTFEKIGEV"
     gene            18740..19189
                     /locus_tag="U2K71_002069"
     CDS             18740..19189
                     /locus_tag="U2K71_002069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316102.1"
                     /translation="MTDIVKIKQSNVQVYPQTHWNAIEGKPTTVKGDKGDPGQAATIT
                     IGTVSSGSTASVTNVGTSSAARFNFVLPKGDKGDPGINATTTAVATTTANGLMSSTDK
                     TKLDGIAAGAQKNPGNATTTTAGLMSATDKAKLDGLANITFEKVGTV"
     gene            19189..19683
                     /locus_tag="U2K71_002070"
     CDS             19189..19683
                     /locus_tag="U2K71_002070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316103.1"
                     /translation="MADIVQLKEDGVAKYLKTHVEAIDGKEALVQTDGDQAIAGHKNF
                     SGSVTINNKRVLTTDDNRYEVVNLVVTNGNTGTAKLYREGKTVSIYFVALNGKSSGGN
                     DSVILTVPEGYRTPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSAYSFVI
                     TYSI"
     gene            19697..20128
                     /locus_tag="U2K71_002071"
     CDS             19697..20128
                     /locus_tag="U2K71_002071"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316104.1"
                     /translation="MKVVYKSIKPYGFEQIILNNQENIPENCTEIKPPVPNWRPRFDF
                     DKKQWVELATEEEKKGNAVDSVDELANIKALYETLKAENDELKQLNSKAMLNNVAIKQ
                     ENVLLKEKSESLAQLNSKTMLASVQNTKEIEEIKKQLQGGK"
     gene            20130..20267
                     /locus_tag="U2K71_002072"
     CDS             20130..20267
                     /locus_tag="U2K71_002072"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEM7316105.1"
                     /translation="MYSYEDIKLMYDWGCFTNEQVMVFVPLCITEEEADKIISKEESA
                     S"
     gene            20305..>20549
                     /locus_tag="U2K71_002073"
     CDS             20305..>20549
                     /locus_tag="U2K71_002073"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316106.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLV"
BASE COUNT         7429 a         2837 c         4233 g         6050 t
ORIGIN      
        1 atcgttatat caatggtctg cccatttatg taactgctca aaggaataat actagcgaag
       61 aatcggttaa agtagaatca aacagaatta tcatccaatt tgcttcatcg ttagaactgg
      121 ttgctttcaa gtagccagtt tttacacttt ttataccctt ttattaccaa tttggtttcc
      181 attttatacc ttttttatac caatcactta cctattcaac gttgtattat gatagtgtcg
      241 aaaaattagg aaacaggatc gacaaaataa aatgtaaggg aggaaatctc cctcatcgtt
      301 taattaagct tcgataggca gcaacggaaa tattaagaat aaggatgtga atttcaactc
      361 cttctaaatt gttcttatta tctatcatcc gttgctgtct attgtttttt taattattca
      421 cacgataact aaaggtaagt gaagagaaat gattccatta ataatttcaa tttttgcgct
      481 ttgtcttaat gtatatatga ttggatttaa aaatgggcaa aataaaaaat agtagcagcc
      541 aagaataatt ttatagtgtc actgtggcgg aaagggtaga cgctaagcat gtgtgctagg
      601 tcaatgcttc ggcaaccatg caaggttcga ttccttgcca gtgactttgg caaccgaggc
      661 atcggcggtt taaaaatata ggggtgcgca atttcgtacg cgttttgtgc atcgtgtagg
      721 ttgctattac ataactggtt aggttagatt gagatttggg attcggtaca aatgaatcgt
      781 caaatgtctc aagcacagga tcggaaacgt ccctgcctgt gcattacata ttagatcact
      841 ctttgagtgg tctttttctt tacataaagg aggctacata atgagaaact actggtatgt
      901 atcgctaact aatgaatatc ctcgaaccat tgatgattgt tcagtgcgtg ttgtgcgttc
      961 tgtacaaatc aaagggaagt actctattgt cgaaatgggg agagaagcag aaccatgtga
     1021 gattgatgcg tgcaagctcg tttattgcgg tcatggtttc tatgatgaac caaacattca
     1081 aaataatatt aacaagaatt tgagggatta gaatgcaaga aatggcttaa aaatctccaa
     1141 tcaggacaaa tcaagttaga caaagtttcc gatttaaaga tattgattga agctgattta
     1201 atgctaaaag atattgaaaa ttagaaaaca aaactcaacc taaacagaat gtgaggtggt
     1261 gtgtattgaa tggcaagaca acgtgatcca agacgtgatg aagccaaaag aatttggtta
     1321 gaatccaacg gagaaaagca gttaaaggaa attgcatctg aattaaatgt ttcagattct
     1381 caggttagaa aatggaaatc gcaagacaaa tggagcgctg aattgattag taacgttacc
     1441 aatggcaaaa gtaacgttac taatcaaggt ggcgctccta ttggtaatca aaatgctaaa
     1501 ggtaacaaag gaaatagccg agcttctccg ccagtgggta ataaaaacgc tttgaaaaca
     1561 ggtgagtatg aaacaatatt ttttgacacg ttaaccgata atgagaagga catctattct
     1621 agtttgaatg atgatccttc ttttgctttg tccgaagaaa tacgacttct taaaataaga
     1681 caacttcgaa tgatgaaaag aatccaacaa gctgaagctg gactaaatga tgaagaagtc
     1741 gaacgattgc agcagctaag aaagattaaa actccgattg aaaaaaatgg taaaaagcta
     1801 gaaatcaagc gtgaggttat gcaagatgtg cagattagca gaaaaaaaca tcgcaaaatt
     1861 gatgatattc tttcaattga agattcattg actcggatta gcaaccagtt agctaaagcc
     1921 atcaagcaaa tgaatgaact ttatatgaat gaatacagaa ctgatttaat taaagctcag
     1981 actgataaga tccaagctga gacaaatgaa attggcggaa ataattcagg tgaagaaata
     2041 gaagaatgga aacaggcagt tttaaatgcc gcaaacaaac gggcggtgaa agaaaatgaa
     2101 taatgaattt attccttttg ctgatattgg ttctgccatt gattattact atgataaacc
     2161 agtagctttt tgccaagata ttttgcattt gaatcctgat gaatggcaag aaaatgtttt
     2221 aaatgattta gctgaatttt caaaggtttc tgttcgttct ggtcaaggag ttggaaaaac
     2281 agcattagaa gcaggagcaa tactttggtt cttaacgtgt cgaccctacg ctaaagtaat
     2341 agcaacagct ccgacaatga agcaacttta cgatgtactt tgggcagagg tagctaaatg
     2401 gttaaatgat agcttgatca aaaacttact gaagtggaca aagaccaaaa tttatatggt
     2461 tggtgattca gagcgttggt ttgctacggc tagaacagcg actaaaccag aaagtatgca
     2521 aggttttcac gaggaccata tgttgattgt ggtagatgaa gcttctggtg tgtctgatcc
     2581 aattatggaa gctattcttg gtacgctatc aggttttgat aataagctgt tgatgtgtgg
     2641 aaaccccaat aatattgaag gtgtctttta tgattcccat aattcagacc gtgataaata
     2701 cagagttcat aaagtatcaa gctatgatag taaacgtaca aacaaagaca atatagaaat
     2761 gattcttaaa aaatatggaa aagaaagtga tgttgctcgt gtccgtattt ttggagaatt
     2821 tccgaaaggt gcgttggatt catttatcag tcttgaaaca gttgaattgg ctacagaaaa
     2881 acaaattagt gattctttag tcaataaaac aaaggttgct catattggtg tcgacgtagc
     2941 tcgatatggt gatgattcta cgattctctt tcctagaatt gctaccaggg cattggagta
     3001 tgagaagtat tcaaaacgta gcaccatgga aacaacagga tatgtcatca acatggccaa
     3061 gaatctaatg agtcaatatc cgagtattga taaagtgatg attaaagtcg atgacactgg
     3121 tgtcggaggt ggtgtaaccg accgcctaga agaacttata gaagacaaac attatccttt
     3181 tgaggtgttt ggagtgaata acggttcaac atcagaagac gatttttacg ataatttagg
     3241 tactcaacta tggggaaaca tcaaggaaat gttagaagaa aatatgacag caaatcttaa
     3301 cggagaacag cctgttattg aattgccttc tgatagttcg ttaatcaaag aattaagtac
     3361 tcgcaaattc aaaatgacaa gtagaagtcg tatacgttta gaaagtaaag atgatatgaa
     3421 aaagcgaaat attggtagtc ccgatattgc tgacgcactg gctttagcgt tttatgagcc
     3481 accaagtcac tatcaattta ttcaatttta ggaggtgagc ttttgattag tacagtatta
     3541 tctttagaga gatataagaa gctacgtgtt aaatatgcaa cacaaattga agatggaatg
     3601 tttgatccga atggttttat agaagatatg aaaccatttt ttgctgatcg cgaaagaaaa
     3661 tatctagctt atactagcga aaagaatgaa atagataata gaccaaagcc taacactgat
     3721 attgtaaaag ttaataataa acttcatgct ggtatgtatt caattgtcgt agaccaagca
     3781 gtcaatcatt ttactggtat acctatcaaa tgggattacg atgtatcgga acaaaaaaga
     3841 acactcattc aaagattaaa agataaattc ttaaaaaatg atatagaact tccgacagtt
     3901 ccagaagctt ttaacaaatt aacaagcaat cttgattcca tgagattcgc aatgcttgat
     3961 tctgaaactg caacttttca aggagcttgt ggagtagctt tccgattgct agagcctgtg
     4021 gaagaagatg atggttggaa gttaagagca agtaatattg aaccttggag agcagaaaga
     4081 tatggaaatg ccggaatcta tatcaaagaa aagtatgact cttaccagaa aaaatttttt
     4141 caagaaatga aagttatgac aagaaataaa attctgacat atgcttgcta tggtgatttg
     4201 aattttgtta caagcggaac gttcaaaaaa attgatgaga ccgataaccc tttggggacg
     4261 attaccttgg cagaatttaa aaataatacg aatcgttatt gtgattttga agtagctgag
     4321 gaaattggag atgcaattga tcgtgctttg tctgatcaac aaaacgaaat cgaacaattt
     4381 aagcttgcgt acatgcttgt tactggcaca acaatgagca aaggcactgc taaagaaatg
     4441 atgaatcagc tagggattat caacttaaaa gcccctactg caaaagctga atatgtcaca
     4501 aaaaatttag caaaagattt caatgaatat catatggatc tattgaaaaa acagttttac
     4561 accatttgta aagcaatcga ttttaacgat gaggtgttta aatcaaacag ttctggagaa
     4621 gctcgcaagt ggcaaatcat tagcctagaa gcgaaaacaa atactaaaga gcaatatttt
     4681 agagaaggat tgaaagaatg cgcagaaaca attgctgctt ttcttaagtt tcatgacaaa
     4741 gtagaaattg agcctgaaaa aattattttt actttctcta gatctttgcc aactgattta
     4801 agttatttag ctgaagcttt acctaaactt gcaccatacg tatccaaacg aactattcag
     4861 agccaaattc catttgtaac agatgttgat tatgaaaatg aaatgatgga attggaaagt
     4921 ggaagtgctt atccagatag tgaatacaat tttggcggag gtggcaatag tgacgataga
     4981 aacgaagtat tggaccaaac gtcgagaact ggaggatcaa gcaaggctca aacaagaaaa
     5041 tcagacactt aaaaaattaa ctagtgtatt tcctgaagca ctgaaagaga tacaagcaaa
     5101 actattatca caagctgact tacacaatat cacttatcca gaaatgatgg agttttacag
     5161 tacaagtaat cagaaaaaat atcgtgaata tgtggaaaaa aattataagt cattaaaaat
     5221 gtatgatgca aaatacaaag agtttatcga tgaatttttt ccaccatttg actatgcaaa
     5281 agtcaatcgc ttattacaaa tacgatcaga tgtatttaaa attcttgcag aatatgcgat
     5341 ggatgcagat gtgaatcaat atttttctga tcgcttagag gaaattcttc aaagaacata
     5401 ttcttctaat gctaatgttt ttgttcaact tttaaacgtt gatataccaa attatttacc
     5461 agaaaatgaa attaaacatt atttgaacta tccatggtca ggaaagacat tttcaagaag
     5521 actttgggga aatatttcat cgcttgaaca gaaactctct aatgctattg taaaaagtgt
     5581 tgctagtgga gaaggcgtta tacacgcatt aaacaccatg agattagact cagaaatttg
     5641 tgacatgttt aagttagaag aatcaaagta taacaaagcg atagaaaatc tcgttcgaac
     5701 ggagtatgca aaatttgcac aagatggtat tgaaaaatca tatttagaaa caggtattga
     5761 ggaatacaac gtattgactg caaaagatga gagagtttgc cggatttgtg gaggaaaggc
     5821 aagtaagaat ccctataaac tgaaagatgc tgtcataggt gaaaatcgag cacctttcca
     5881 tagtcgttgt agatgtacgg atgttcctaa tttaccaaaa ttaggaaagg atattgatga
     5941 agaatatgac cgtttatttg gcgatttatt agatgagttt gcacatgatt cttttggaat
     6001 taatttgaaa cggaggaagt agaatgaaag atttttttga agcagtacta acaattaatg
     6061 taaatgctga tattgcagaa gcctacaaaa cagctattga gtctgagaac catcctaatg
     6121 gcttgagaga ccattggaat ggcaattatg cctacgtggt tattggcgat caaactgtta
     6181 attatcaaga taatactcca gttgataaga ataccgttaa tttaacgatt caattattat
     6241 ctcattcatt accaaattta aaagaaacag ttgattggta tgaaaagatg ggatgtattg
     6301 ttgttagaac tgactacaaa gaaggaaagt ctagtaatta ggctttttta ttttgtccga
     6361 aatgacgtta aactagcgca atactgggct tggttgaatg gtggggcgca actattaaaa
     6421 ctcaaagcaa tgcggggcgt gaaaacgaat cgtggggcga aaggagaatg agcatgaaac
     6481 acaaatcatt aatgccaatg aatttgcaat actttgccga aggtgacgat caaaagtttt
     6541 cttttgatga cttcaaatct tttgtggaat ccaatgaaga agctcaaaaa tttattcagt
     6601 cacaatctca aagtgttgcc gacaaacaat tggaagcttg gaaacaaaat aacttagata
     6661 aaattaaaca ggataccatc aaggaatatg aggaatctaa gaaaaacaag tcacctgagc
     6721 aaattcaact ggaaaaacta caagctgaat ttgaagcaga aaaagcgttg cgtgtgacaa
     6781 gtgataataa agcatttgtt gcagaacaaa ttgctggatt agaactagat ggagagttaa
     6841 aagagtctat ttctcaattt atgctaaata atcttgttag ttcggataca gatttcacta
     6901 agaatgctgt tgaaggtttt acaagtgttt tgaatgcaat taatgagaaa catgcagatg
     6961 cattaaaaga actacaaatg aagtctgcat ttggtggaac tcaacaatcg aataaccagg
     7021 ttcagcagaa caatgaaaca ttaacaaatc cagaagaaca attaggacaa attcttcaac
     7081 aatttaacta ggagagtgaa aaattatgaa aaaaacatct ttaaataatt tagagtattt
     7141 ggatatttca ccagcgatta atgctatgca agtaccaaat acaccttttt taagctattt
     7201 acttggtgct ggaaaaacag agccagcaaa ctcgacagaa attaaatggc gtgaatatga
     7261 tatcaacaac gatgattctt ctgaaaaact tgagggcgga gaatatccag atgctgaatc
     7321 aggtcgaact tggtttaaca actatactga aatttttaga aaatcaacct ctgtatctgg
     7381 tacattagat gctattaatg tgaatggagt cggaaatgaa ttaactaatc aagtagcact
     7441 acgtggtatg gaaatgaaaa ttgatttgaa ccgaaaattg attactggtg taaaagctga
     7501 tgaaaatggt tctaaaggtc gtcgaatgaa tgggattttg aacttgatca attcagcaaa
     7561 taaggcagaa acagccactg cgggtgcagt aacaagaaaa gatatcgata ctttatttaa
     7621 attgatgtat gaaaaaggtt atatgggaga aaaactatgc ttgatttctc cagatatgca
     7681 ggagttaatg actgatgagt tagatggaaa atcaacaaaa attgttcagt tcggggaaag
     7741 agtaactttt ggattgcaaa ttggaaatat cgtgtctaat tacggtacag gtattgctct
     7801 actagaacca tcattgccaa aaggaacaat tgccgcaatc gatactaatt atgtgaaact
     7861 acgtccatta cgtgaatgga gagcagaaga acttgcaaaa acaacggatt ctagacgtat
     7921 cggtcttgta ggtgaatact ctcttgaata caatgcttca aactctgggg caattttaaa
     7981 tttaaagtct gaataaaagg gagttagtac tccctttttt gataggagga attacagtgg
     8041 taaaaaaaga tgaaactaaa aaagatgaag tcgtgaaata tagagtaggt aaaactaaaa
     8101 attttgttgg gtttgttcat cctaaaactc gtagatttat cacagcagat tcaaataacg
     8161 aatttatcat ttctatagat gataaagaag caattgcaat tttggaagat gcaattgatg
     8221 ttaatgaaat ttaggaagtg atctgatgga tgaatcgcta aaaacggaaa tcattgagtc
     8281 tacaaaagaa gattttccag atttgagtga agaacgcata actaatttat tagaaataat
     8341 tttgctagaa attgaatcat acaacacttg taaaaatgat atttcatggg aaaagttaaa
     8401 aagcgtgatt agtgaagtgt tgtataaaat aataaaaaat gaatcagaaa aaacagtatc
     8461 ttcagttaga cgtggcgata caacgattag ttatgcttca acaacaaatg atgttagtga
     8521 gttacttcta ggctatggtg atttgatacg aagagttatt ggttgtggag gattggagtt
     8581 tttttaatga atgaagcaga tattttagaa actacttatg aagatagttg tattattgaa
     8641 agactgacgg acattgaaga ttctaataca aatattacta ttcaagatta caaaaaagta
     8701 tatgataatc ctatttcttg cgctctttca caaggtcaaa tcgatggact agcagtcata
     8761 gaagatggag agatggtaaa tgtttcaact gacacatata aattatttgt tcatcctaag
     8821 attaaactaa aaaaaggaga tcgaataaca ataactcaaa aggccagtgg cttaattttt
     8881 tctctatttg ctactaagcc tttttactat cctagtcatt gtgaagtgaa tttgatagga
     8941 agtgagaaaa atgggtaatc tcaaatatga atcaaatgca gaaaagatca ttgagaattt
     9001 taaaaatatg actgtaattg ctcaaaaaga aggaatatcc ttcgttaacg attcaatgaa
     9061 taaagtcgtt agtttagcta aaccattaac tcctgtaaaa tcaggtaatt taaggcgagg
     9121 atatcgtgta gtaaaagcta gaaaactatc aagcggtcga attgttggag aagttataaa
     9181 caatgaacat tattttaaat atgtagaaga aggtcatagg actaaaaatg gtggctttgt
     9241 caaagggaaa ttcatgttaa ctcgtgcaac gaatcttgca aatatgtctt atattcctcg
     9301 aagatttaaa caaatggcaa taaaaatcgt taagaaagga aagtaacatg aaagatgaaa
     9361 tcattgctgc aatcagtagc aaattaaaag aaatctatcc tgatgggaca atatatcttg
     9421 attcagttat gcagtcaact aaagattttt atttcgtttt atcagtaatg gaatctggaa
     9481 ctgaaaatgt aggaattgat attcaaaatg tttctttctt aattgatatt gcattgattg
     9541 ataataaccc taatagaaat ttaattaatg aattagtctc acgttgtggg acttttttta
     9601 atacgattac aatcgatgag caaatattat ttccaaaagc ctatttacct gatgaagcag
     9661 atggtgttca acatattcgt tttacattag aatttccaca atatattgaa tggagtgaaa
     9721 gataaatggg agaaaaaaga agtaaaaccg gaattatttc tgtcgaaaaa cctacttggt
     9781 ttcctttgga agatgagaca gggaattttc cggtatatgg tactgcgttg ccaatgggaa
     9841 cagcagtaag tatcaaacca acagctaatt atgaaacaac acaagattat ggggattcag
     9901 tggttcaaga tcaatttaca gcttttggtg gtgcagaagt tgaactggaa gcaaatggat
     9961 atactcataa agttttatct acaattactg gagggaaaat tgttaaaggt ggtgccttgc
    10021 gttctggaga agatattgcg caagatggtg cgtttgcata tagacgtaaa aaatcgaatg
    10081 ggaaataccg ttatacggtc ttttataaag gacagtttgc tctagattca gatgaaacat
    10141 caacgattga aggaagtaaa gtaagtttta ctcatccaac ttggaagggt tcatttgtag
    10201 atgtaccagg acttggatat atgtattcag ttgatgaaga tgatgaaggc gtggataaag
    10261 caatgatcgg aaattggttt acaaaagtag caattccaat tgaagaagca gaactttcag
    10321 gaggtacaga ataatgtcaa aatatcaaac aacgattaaa ttaacgaaaa aagacgaaga
    10381 aggtaaatat gagcaagtac aatttaaatc tgctgaattt ttaccaggaa cagttgtaga
    10441 agatgctgca ggagttatgg aagaaatgca aacagcgact gataagcaat cagttaaaaa
    10501 agctttaagt cgtgcttatt catttattgc agatacttta tttgagggac aatttactgg
    10561 tgaagattat tgcaaaggaa ttgatgctcg tgagattgcc tcattgacag gaaaattatt
    10621 gaagtctgtt actgcaggtt ttgatgaaac ttatacagag acgaaaaaaa agtaagtgag
    10681 gcgctcaagt caccttcatt taagtattcg attacttacc gagaattaga tataaagacg
    10741 caattactcg aagcaggttg gacgttacca gaaattgaac gtactgactt ggatgagtta
    10801 atgcgtcttt ttgcttttag agatgcggta aaagagcatg aagatgttga gtactatgat
    10861 aatttcactc aattttagga ggtgatactt tgaataacga tgacctaatt ctgaagatga
    10921 tactagatga atctggtttt actgccggta tgaataatgc tgtcaaaaag ttgaactctt
    10981 ttgatgaaac aatcgaaaga aaaagtagaa atagtggcag ttcattaggt agtatttgga
    11041 aaatatttgc tggaagtttt ctagctagcg gagtaactag aatcgtagga gctggctttg
    11101 atctaatcaa gggttccata agtggagcgg ttgatcgagt agatacgatg aataacgctc
    11161 tacgaaattt ccaaaatatg ggatttagta attcagaaat tatgaagaat atcggaaaga
    11221 atgggctttt atctcaaggt attcaaggac ttcctactgc tttgaatgat gcgataagtc
    11281 atgttcaact tcttgcttct tctacaggtg atttaactcg ttcaactcaa atatttaaag
    11341 ctttaaatga tggaattctt ggtttcggtg gttcaactga tcaagttaat gaagctgtta
    11401 ttcaattatc tcaaagtttc tcaaacggaa aagtagacgc acaaacttgg aactcaatga
    11461 tcaacgctca acttggacct actctttctg ctattgctaa aaagatggga attacaatgg
    11521 gagatctgaa agaaggttta tctcaaggta agatttctgt tgaagaattc caaaatcaat
    11581 taatagaaat ggataccaaa ggtggcggag gacttaaatc attaagtcaa atcgctaaag
    11641 attctactaa gggaattaaa acctctatac aaaatgcaaa aacagctgtt acacgtggtg
    11701 ttggtgaggt aatagaagga ttaaataaag cattagtaga ttcagattta ggtggattta
    11761 agggaattat tgataaagtg ggcagttcaa tggaatcgtt cttaaaagta attgctgcaa
    11821 atattcctac agcagtatca tttttaagta atttatttaa cgaaattcaa aaatttggtt
    11881 ctgctttgaa attcatgatg ccctttcttg ttcctgcagc taccgctttt ggagcgttca
    11941 tgtttcaact taaaggtata ccagcaataa taaaaagctt caataatttt aagaatgcca
    12001 taattggcgt tggaaattca ttaaagatta tgggggcaat agctgctgct aatccatttg
    12061 ttttaattat tggtgcagtc gttggtgcaa tagctgtttt cggttacttt atggcaacca
    12121 atgaagagct tagaaacaag gttatatcta tttggaatga tgtgaaagat tccgtaattg
    12181 gtgtattaaa aaatataaag gactggggaa ttgatacttg gaattctgct aaagaaatgg
    12241 catcaaatgc agttgagggt gtcaaagatg cttggtcagg aacaaaggaa tggttttcta
    12301 acacctggaa aggtattaag gatggggcca caggtttatt tgataaaaca gtagagactt
    12361 caagaaatgc agtcgatagt gtaaaaaatg catggtctaa tatgaagaaa tggttttctg
    12421 atacttggca aagtataaaa gattcagcca gagaaaaatg gaatggaatc aaaggttcta
    12481 ttatgtcagt tgctggtcca ttgattacag gtataaagaa tgcattttta catgttactt
    12541 tttatctaga gaccttatgg aataatttag ttgagattgg taaaaacgtc tttgagattt
    12601 taaaaaatgt tatacttgcg ccggttttgt ttattacctc catgatttct ggtggatggg
    12661 aagaaacaaa aaataatatg attgctgttt ggaataatat taaagaaagt gccataaata
    12721 tttgggaatc tataaagaat atatttgtaa gttattttac aaatatttat tttgctgcac
    12781 tcaatatttg gacagggttc aagcttacat tgataaatat ttggaatgaa gtggtaaatc
    12841 aagctaaatc gatttggtta aacataaagt atttttttat taatctttgg attgatataa
    12901 aatattttgc aattcagaaa tggattgaat tgaagttttc tattattcaa acttggatcg
    12961 atttaaaata taatgccatt actacttgga ataatattaa acagtttttc aaagatactt
    13021 ggaaaaatat taaagatacg gcatacaata cgtggatttc tataaaaaat accatgatta
    13081 atacatggaa taacattaag gactctttct ggaatattgt tactggaatt gttaattctg
    13141 ctgaaaatgc atggacaaat ctaaaaaatg gtgtttcaaa tgctattaat cgggtaaaag
    13201 aaatctttaa ttcgttaagg gaagtcaatt tatttgaaat tggcaagaac atcattgatg
    13261 gacttataaa tggtgtaaaa gaaaagtgga atgctttgaa agatactatc aaaggtattg
    13321 caggtaatat caaaaattgg attaaaggag ctttagatat tcattctcca tcaagatgga
    13381 tgcgtgatat ggttggtaaa aatattgttc aaggtattat cgttggtatt gataaagagc
    13441 aaagtaagtt agatcaaacc atgacaaatt tagtaaaaac accgtctgtt caacctgtag
    13501 taaatggaat aagtcaccta ccaattacac aaacaaagca aaatatatct gctagtgaaa
    13561 caaaagaaat tcatttacat ttaaatgttt atggagattt acctgattca atggttcgga
    13621 agattaccac aaaaattaaa acagaattga caagacaaat gaaacgagat gctggtgcag
    13681 taggaggaac attatatgca acttaagaga ggacaatttt ttattaatca acattattct
    13741 tctgaattta atgtgtatat tcaaaacagg cctgcttctg tttcagctag tcgagtaatt
    13801 gaattgagag agcgagaagg aaatgattct atcattattg ataaaggcta ttacaaaaat
    13861 gtgactagaa agattgagtg ttactataaa gcaccttcaa ttgatatggt gcaggagtgg
    13921 gaagatcgaa tcactgaatg gttagatatg tcgtcttata gtgattttat tctttactat
    13981 gatgaacaat atatttatca agctgtagta atagaaggac ctgaatttaa aggaacaaga
    14041 aaaacaggaa atattgtccc ttttgaattt acagtgagta ttcgaccatt taaggaaaat
    14101 tacaatggta gatttactat tcgacaaaca gaaacttttg aaatttataa cccagagaag
    14161 tattcttcaa aaccgcttat taaattgagt ggttctggag atgcttcttt ttatattaat
    14221 aaggataaat atgatttgaa atcgttggac agagaattat ttatagattc taaattagaa
    14281 gaggcttatc gaaaattaga tggtaattta gaacatcagg atcaggtcac cttattttta
    14341 gattttccat ttttatatcc tggaaaaaat gaaattaaat ggacgaaaaa tattcattca
    14401 tttgaaataa tgcctaggtg gtggagaaaa gtatgaaacc aagaatatat agtcctactg
    14461 aaacagattt ctcaacgaat ggcttaggaa ttttaaagga ttgtacaaga tgtggaatat
    14521 atgaagtagc taatggaaaa tatgaattag agttggatta tcctttagga actagatttg
    14581 atgaatattt tgaaaatgac tatcaaataa aagcaaagcc aaacgatcaa gaagagtatc
    14641 atatcttttt tattgatgat aaagatatag atactttttt aaatacagta actatttatg
    14701 ctcagagtcg tacaaatcga cttggaagac gggtagtcac tcttgtggaa gtggactcta
    14761 aaactggtca agaagcgata tcaattattg aaactaaaat ggataaaaaa tctgacatac
    14821 gactttattc tgatattacg gctgtttcta gcacaatctt tgaagcgaga aatgttttaa
    14881 attgtattgc tggtgaacaa ggatcattgc ttcaatattg gggcggagaa attaaacgtg
    14941 aaccatttaa gctttctttg ttaaagcgaa gaggtcgcaa taatgttgga acgattcggt
    15001 atggaaaaga tatgtctggt ttgaaggtca aattagattg gacaggtata aaaacaagaa
    15061 ttattccata tgctgatcct caaagtgaag taggtacgac taatcgaatt tatggttcgc
    15121 cagtagatag tgcctatatt aataactatc ctgatgtcta tacagaacat gttcagttta
    15181 cagaagaaca aggagtaaaa gacattaaga gcttaaataa aatagctaaa aattacttta
    15241 aaacgatcaa tcctggctgt gataagccta aagtttctat tacggttgaa tttgataagt
    15301 tgactgatag tgaagaagcg aaagaatttg cgaagattag aaattatggt ctgtttgata
    15361 catttaaaat atatcataaa aaatataata tttatcttga atcaaaggtt agtggactac
    15421 aatacgattc tttatctgaa aaaactttga aattagaagc aggagacatt caagttgctt
    15481 tttatcagca acaagctgta actattcagg ataaattaaa agattatgca acgaataatt
    15541 atatgagtga ttttaatgat tatgtttctt caatgattac aggtcaaggc agtgcaggag
    15601 gatatgtagt tttatggcca aaagaaaaac cttccaacat ctttattatg gataatccag
    15661 atttagaaaa ggcaagagaa gtactaagaa tgaataaaaa tgggattgct ttttctaaga
    15721 aaggatggcg ggggcctttt aattcagctt ggacattaga tagtatattt aatgccaatt
    15781 ttatacaaac tggattaatc aaagcagata tctttcaaaa ttcttttaac aagactggag
    15841 atgtattgaa attagttaat ggactactcc aaatttggaa caacaaaaag aaaataatgg
    15901 agttaactaa aaaaggaatg gaattttgga attctaaggg ttcaattgga acgattggaa
    15961 caactgattc tgttggtaat ccttttcctg gggcttctac tccaacccct attgaagata
    16021 attctttagt tattcgtaca aatggagacg gcaaatatat tttgatttct cctaaagtag
    16081 gaaaaggatt agttttatta ggaaatggta aagcaattta ttttggagac ttagatgtac
    16141 aaggaaaact tacagttaaa ggaaaagaaa taattcctgg acaaggtggt ggtccatcgg
    16201 gaggtggaga atctactggt gggtatccta gtgaattaaa aacagatgca gaaaagagag
    16261 cttggagaat ttacgatatt ttgtgtaata acggatttac gaaacaatct gcatgtggaa
    16321 tattggggaa cattcaacaa gaaacaggag gaacttttga tcctgatact gttcaaatag
    16381 gtggaccagc atacggatta gttcagtggg atggttcttc atatcctctt gttggtccag
    16441 caacatggga tggaaaagtt tatgttcaaa acttatttaa tgctgcaggt attaaagaac
    16501 caataacgag cttagatgca caagttcgat tgcttatttg gacatttaca aatggacaat
    16561 ggatgggcgt agtacaacct acgacggttg atggatttaa ggcctgtact gatccaagac
    16621 aagcagcata tgcttttgaa cgaaactatg aacgtccggc agcgacacat cctgaacgtc
    16681 aagattatgc agttaactgg tataacaaat ttaaagattt aaaaccagga ggagctactg
    16741 gagaagcagg actaaaacat ttagagtctt tgattggaca acgaattggc aatggtcagt
    16801 gttatggctt gtctgcagaa tattcaggat atctgggtgg ttgtggcatg ggtgctggaa
    16861 caaaatatgg tttaactcat gtgataggaa atacttctgc agcatctgat attggtattg
    16921 cctatgattg gtctgctgtt ggttggaaag tgattcaaaa ccctagatat gatcaattag
    16981 tagttggtgc aattattaat tgggcaagag gtggacaagt gggttcatgg tttgcagatg
    17041 gaacttatgg acatactggc gttatccgag gcttagctaa tggtcgtatg caaacttatg
    17101 aacaaaacac agagttaggt atgatttgtg gaaagttaga tcgccagtat tatagtgcaa
    17161 gtgcaatttc ttccattgtc ataccaccaa aatagggagg tgatttaatg gcaaagtgga
    17221 atgtcatatt aagtacaaca gaaccctata attacgtggg gatgattcaa gttcgacaag
    17281 gcaataagaa tacagaggtt atggaagcga ctatagttga aaatggtctt ccctacgatt
    17341 tatcagaatg taaggtatat tttgaatcag ttgtaggtgg gaaatatcca gtccaattag
    17401 aaacaaaaat tgtggatgct aaaaaaggga aaattaacta tatttttgat aaatattcca
    17461 tgcagtgttt acatcgacaa acagccaatt tcattatatt taaaggagaa gacttgattg
    17521 gaacaactca agacttctct tattttgtca ttaatgctgt ttcaaaaaca gaaggagaaa
    17581 tgggttctta ttggcaatca atcgaagatt taattgcgga tatgacggac tttattaatg
    17641 aaaataaagg cgattttacg gactggatga atgaaaggaa agaagaattt gaacgttgga
    17701 gacaagaaca ggaacacagt ttccaagatt ggagagaagg acaagaatca gactatttgg
    17761 catggtttga atcaatcaag gatattttaa agacgattga tccaggtgga gtcatgttag
    17821 ccgaactaac ggatgcgcga gtagatattc aaggagttcg tcatgcgtct atttcagagc
    17881 gtttgctggc agatatggac tatttatatc agaaattgcg agcaacgctt ttcacgattg
    17941 aatacgggga gattgaagtg actgatattt tgcaggatga tctcttttca gataatcatg
    18001 aagtcgaaaa agttggaact gtagcattcc cgattgaaga aggagccttg atcatcgcaa
    18061 ccgttgatga tccaaagcaa aatgtgttca ctcttgagaa agttggggtg atctaatggc
    18121 taaaactaaa cgaatgatgg aaacggatga aaaaacgggt gtacaacgcc aattctttcc
    18181 aatcacacat gcttccgcag ttcttgggtt agaagaaata atagcaggag aagcaacagt
    18241 tttatctgtt aatggaaaaa ttggagccgt cgtcattact aaagaagatt tgggattaga
    18301 gaatgttctc acagaattac cctatgcaag tgaagaagat gacggcatta tcacggcaga
    18361 aatgtatcaa aaaattttaa acagtggaga aggtgactac gtgttgccag ttgccactat
    18421 cgaccgtttg ggtggcataa aagttggtga attattgacg attgatgaga cagggaaagt
    18481 ctctgcagtc agacaatctg atgtcaattt ttcgctggag ttaaaagaaa aactcgattc
    18541 actgaaaaat tatactgctg gagaaaacat cactattgat gaagatggaa caattagttc
    18601 tacaggtggt agtgggactg gtggtgtcaa tcagtcatat gtagatcaaa aattccaaga
    18661 agctgtaaat caagcagaaa attacacaaa cgaaagaatt ccaaatttta cttttgaaaa
    18721 aattggggag gtatagagaa tgacagatat cgtaaaaata aaacaaagca atgtacaggt
    18781 ttatccacaa actcattgga atgctataga aggcaaacca acaacggtga agggagataa
    18841 aggcgatcct ggccaagcag caacaattac cataggaacc gtttctagtg gttcaaccgc
    18901 ttctgttacg aatgtaggaa catcatctgc agctagattt aattttgttt taccaaaggg
    18961 agataaaggt gatcctggaa taaatgccac aactacagct gtagctacaa ctacagcaaa
    19021 tgggttgatg tcgtccacag ataaaactaa gttggatgga attgcagctg gagcacagaa
    19081 aaatccaggt aatgctacaa ctacgacagc aggtttaatg tcagcaactg acaaagctaa
    19141 actcgatgga ttagccaata ttacatttga gaaagtgggg acggtttaat ggctgatatt
    19201 gtgcaattaa aagaagacgg agttgctaaa tacttaaaaa cgcatgtaga agctattgat
    19261 ggtaaagaag cattggtgca gacagatgga gaccaagcca ttgcaggaca taagaatttt
    19321 tcaggttctg taacaatcaa taataaacgt gttttgacga ctgatgataa cagatatgaa
    19381 gtggtaaatc tggttgtcac taacggtaat acagggacag caaagcttta tcgtgaagga
    19441 aaaacagttt ctatatattt tgtggcttta aacggaaaaa gtagtggcgg gaatgattcg
    19501 gttattttaa ctgttccaga aggctatcgg acaccaatta gttttgaaca actggttggg
    19561 tcaatagacc gttctacttt gaacagtgct caactatcta ttggcgcaga cggagccatt
    19621 aaatggagaa gaaattcaag ttatggatca gcttattcat ttgttatcac ttattcaatt
    19681 taagggagga aatctaatga aagtagttta caaatcaatc aagccttacg gattcgagca
    19741 aatcattttg aacaatcaag aaaatatccc tgaaaactgt acagagatta aaccaccagt
    19801 tcctaactgg agaccaagat ttgattttga taaaaaacag tgggttgagt tagctactga
    19861 agaagagaaa aaagggaatg ctgttgatag cgtagatgaa ttggctaata ttaaggcatt
    19921 atacgaaacg ctgaaggcag aaaatgatga attgaaacag ctaaattcta aagcaatgct
    19981 aaacaatgta gcaatcaagc aagaaaatgt cttattgaaa gaaaagtcag aaagtttagc
    20041 gcagttgaat tcaaaaacga tgcttgcttc tgtacaaaat accaaggaaa tcgaagaaat
    20101 caaaaaacaa ttacaaggtg ggaagtaaca tgtattcata tgaagatatc aaactaatgt
    20161 atgactgggg ctgtttcact aacgaacaag taatggtttt cgttccgttg tgcattactg
    20221 aagaagaagc agataaaatc attagcaaag aagagagcgc atcttaattg atgtgctttt
    20281 tattttgatt caaggagttg tcacatgatt aatttagggg aatggggagc gatagcagga
    20341 tcaataaccg ctatcgtttc tttgatttta ttagtaataa aaccaattac tgcatctttc
    20401 tcgaagatta ctgagactct ttcaaaagta agtcacaatt tagatttgct gactaaagat
    20461 ttagaatcga gcaaatcaga tcgattgatg attcatgaag aactaaagaa acacgatgaa
    20521 agattagata cacatacaga aaaattggt
//
LOCUS       DASHHB010000050        20506 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.052, whole genome shotgun sequence.
ACCESSION   DASHHB010000050 DASHHB010000000
VERSION     DASHHB010000050.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20506)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20506)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20506
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.052"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(18..491)
                     /locus_tag="U2K71_002074"
     CDS             complement(18..491)
                     /locus_tag="U2K71_002074"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEM7316107.1"
                     /translation="MVMNAISAMKKTKQQLHEAGFKLTPQREATLLVLLENEKEHLSA
                     EEIFFLVKKKSPEIGLATVYRTLEILTELKIVDKVSFNDGLARYDLRKEGAKHFHHHL
                     LCLNCGTIEEVEEDLLLEVEQVVEERYHFLVKDHRLTFHGICQECYEKEKRNQSV"
     gene            complement(596..1459)
                     /locus_tag="U2K71_002075"
     CDS             complement(596..1459)
                     /locus_tag="U2K71_002075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEM7316108.1"
                     /translation="MNDLLATIFTGIIIDENEKNYFVQKNGQTFGLSKSEGEHKIGEA
                     VEGFGYQNQKHENRITTQIPKSRIGHYAFGIVTATRKDLGAFVDIGLPDKDFVVSLDE
                     LPTMTELWPKKGDRLMISLRVDAKDRIWGSLADEKIFKSLSKYGTEEMKNKDVIGTAY
                     RLKLTGTYVLTEDFYLGFIHPSERYNEPRLGEQIKGRVIGVRPDGVLNLSLKPRAYEA
                     ISDDAQMILTILERSADQQINFTDKSDPEEIMKAFGISKGAFKRALGNLLKQGLITQE
                     NGVTKLVKKSN"
     gene            complement(1655..3754)
                     /locus_tag="U2K71_002076"
     CDS             complement(1655..3754)
                     /locus_tag="U2K71_002076"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TFIIB-type zinc ribbon-containing protein"
                     /protein_id="HEM7316109.1"
                     /translation="MKKCPNCGSKKMEQNTICPDCGHQITAEDQQIKESIEETNTDAA
                     MQDKSTEDAVFPDTELNDPIEWSELKDLPLESVMELFEKAEPEDEEPPAHDQVTNIEK
                     NEQKKATVHETSEESGKNQEETLESKQKRRVAELKEIVDNEEENSILSAYIKAHREDT
                     KEEHAKELLKMISEKIAQENKVEPEKEQETEKDSDRENATGKETVKIKDVKTDITEKT
                     EVFTEKQLEKEKTNKLPEAEKKEILENTDKKSTEPDEINSESDAENQSVSKEGTDLEA
                     KIEKEENQPKLFEAEAKSITIVPDTEKTHQESNLEKTDPDKAQKKKSKKVPYLVLAAV
                     LLLGGGSWAYYDHHQKVEAEVAAEAKRQQQMEELHEKLAAFYTDDHHQFIRADMIHQD
                     LSKLKNDLSSLKNEKGYSELETTYQDIQSKIRDIQKVNEWFIAPAIVDDHLADDIKLK
                     ADKEIQAIETDNTDFGKLIQQAQTEAKNQYAELQTAKEKTAIVFKDGKVVDSATRDQY
                     KTAKEAVEKVKNTDLSAQLNEQLKQVDKMLTDKEKKEEEEKKKAEEAKKAAEEQAKQA
                     AQAAAAAQAAQQAAVAQAAQQEAASSTPSSSNSANQPIKSARASDIADASNPAWTWAP
                     GVQSKVIATCIQRGYIVEGGYRLEKARIENGEGYYNLYATSTKSALMKGIGESALPFY
                     IVTINCKTGWFGGNGSN"
     gene            complement(3974..5083)
                     /gene="rpoD"
                     /locus_tag="U2K71_002077"
     CDS             complement(3974..5083)
                     /gene="rpoD"
                     /locus_tag="U2K71_002077"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoD"
                     /protein_id="HEM7316110.1"
                     /translation="MANGTDKKTYEAAVAAFIKENKPKRQVVYDDLSNQLATPFTLNA
                     DEMDKLIQKIEDAGISVVDENGDPSIHSLKSAEKKAEQAKTEDLSAPTGVKINDPVRM
                     YLKEIGRVSLLTAEEEVALALKIEEGDQEAKQRLAEANLRLVVSIAKRYVGRGMQFLD
                     LIQEGNMGLMKAVEKFDYRKGFKFSTYATWWIRQAITRAIADQARTIRIPVHMVETIN
                     KLIRIQRQLLQDLGREPTPEEIGAEMDLPTEKVREILKIAQEPVSLETPIGEEDDSHL
                     GDFIEDQEATSPAEHAAYELLKEQLEDVLDTLTDREENVLRLRFGLDDGRTRTLEEVG
                     KVFGVTRERIRQIEAKALRKLRHPSRSKQLKDFLE"
     gene            complement(5107..6948)
                     /locus_tag="U2K71_002078"
     CDS             complement(5107..6948)
                     /locus_tag="U2K71_002078"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HEM7316111.1"
                     /translation="MAQRIPQEVIETVRSQTNIVEVIGQYVQLKKSGKNYLGLCPFHE
                     ERTPSFSVAEDKQIFHCFGCGKGGNVFTFLQELEGLSFPEAVVKVADFEQIPLEDQWR
                     QRTIAVQNPESATGKLIAAHEKAAEIYHHMLLHTKAGQPALEYLQSRGLSLELIEEFN
                     IGFAPNERSFLQQVFKNESISNELLERSGLFVTHDDGRMSDRFYQRVMFPLRNSQGQT
                     IGFSGRWLAPEKGQEKDQPKYLNSPETELFNKRQVLFNLDKARSDIRKNGEILLFEGF
                     MDVIAAWQAGIKTGLASMGTSLTAQQIQQMEKLTKELVFCYDGDNAGFEATNRGIELL
                     RQNSRLALSVVVVPEKLDPDEYLRKYGEASFQELVAHGRETVFTFKSRYLKQGKNLDN
                     EKDKIEYLEELLVELSRVVSPIEQDMYLSQLSTEFQIGRETIQKQLRDLRRTNQQNRQ
                     EPQESQHTQVTRQETARKKRPLTQVEKAEQILLYRAFKEVSVRNILKEREFQFIHDAY
                     AEVYFLFDAYVSQHYEFNLAEFLDFLQDENLRRLVVEIEYLPVAEESSPREIQDLLDV
                     ISKSGLADEITLKKQMQQEARRTGNKQLELELTIEIINLTKQLKQAK"
     gene            complement(7130..9445)
                     /locus_tag="U2K71_002079"
     CDS             complement(7130..9445)
                     /locus_tag="U2K71_002079"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM7316112.1"
                     /translation="MNGLTQQEVVQKMASGQQNDYQEDASKSTKQIFSDNILTLFNFL
                     NFGIGICLLFVGAYSNMAYLAIIVVNIVIGIYQEIHARNLVRKLSIVSQSEVLVIRDG
                     KEQSISTKELVLGDLVKIGAGEQIPADLQVISGRAEANEALLTGESDLIVKETGAELL
                     SGSFLTSGSVMAEVIRVGADNYAVRLTSEAKTHKAIRSELVDSIRKVSKFTSFVIIPL
                     GIILFLEALFIRDASIKISVIASAAALLGMLPKGLVLLISIALTTGVTKLAKKRILVQ
                     DMYSIETLAHVDTLCLDKTGTITEGKMSIQRVEALHPLYEKAIPEIMGTYLAESSDNN
                     ITMRAMRDYFELSNRYDVTNKVAFSSDRKWGAMEFPELGVVYVGAPERLVEEDRLPDS
                     LKTAQANGYRVLMLGIAPNQQLNEEKPAFVEPVALFEIDDPIRKNAQETLAYLHEEGV
                     DLKVISGDNPFTVSNIARRAGLPNYDAYVDLSQITEEMEVREAAHRYTVFGRVSPQQK
                     KLLVNELKESGRTVAMTGDGVNDVLALREADCSIAMAEGDGATRQIANLVLLDSDFTT
                     LPEVLFEGRRVVNNVTKVSGIFFIKTIYSFILSIICAVTAIGFPFIPIQITLIDLAIE
                     GYPSFFLSFEQDKRKITYRYLPTALINALPNALLVVVNIIAVYLLGKTFAWGNAETTT
                     LMYYLLIGISCMAVVKACYPFNPLRIFLAVTTIVGIYVAAMLFHGILEVNLGLGESVG
                     YFFVFMLVNIVLRVLYWKADIQNQLLKRVAN"
     gene            complement(9630..10490)
                     /locus_tag="U2K71_002080"
     CDS             complement(9630..10490)
                     /locus_tag="U2K71_002080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF368 domain-containing protein"
                     /protein_id="HEM7316113.1"
                     /translation="MEKRQKMENQEVGYTTKDWMLRFVKGMFIGSGFILPGVSGGALA
                     AVFGMYERLISFLAHLTKNFKENVLFFLPVGLGGVTGIFLLSFLVSFLLGAYETTILW
                     FFVGCIAGTVPALWKEAGKKGRDTSDVIIMVVTFVLAYLFLLYGAQLFDGQVDQNFGT
                     WLIAGGLIGLGMIVPGLSPSNFLVYMGLYKAMADGFKEVRLEVIVPIGIGGILCVLGL
                     SKIMDYIFSKAYSKLFHFILGVVFASTIMIIPTDYAGLGVAGVLACPVLFIAGTALGW
                     WMSRLEEQYK"
     gene            complement(10549..11070)
                     /locus_tag="U2K71_002081"
     CDS             complement(10549..11070)
                     /locus_tag="U2K71_002081"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme family protein"
                     /protein_id="HEM7316114.1"
                     /translation="MESYQIEITTAAKNYHMENYENLIKAIILTESKGKGTDLMQSSE
                     SLHGEAEIIDNPADSIDQGVKYLAEMIVEAKKQGCDLWTAVQAYNFGKDYIAYVKNHG
                     GENTVELAEAYSKDVLSPLLGNEEGKQYRYWRIQSLLYNGGYLYHNGGNMFYPDIVKM
                     NEKIIETYEKVFK"
     gene            11348..12109
                     /locus_tag="U2K71_002082"
     CDS             11348..12109
                     /locus_tag="U2K71_002082"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM7316115.1"
                     /translation="MTDIIVILYTVGDYVKKEKKYAKMPDGSEIYYEKSGQGFPLFLL
                     HGNDGSGRFFSEQVPVLERYYTVYLVDSRGHGRSTNEASMLNFQLMAEDLNTIMLLEK
                     IDQADFLGFSDGANLALVFASSFPKKVHRLILNSGNTLVKGVRFSARVISNIHYAWVW
                     LLSLFRPSLRKNLLVIKLLLHDIGLTENDLKKINSPTLIIVGKKDVIKLKHSLYIAKT
                     IPKASFVLVKEQGHELARKDPERFNREVLQFLSET"
     gene            12135..14726
                     /gene="mprF"
                     /locus_tag="U2K71_002083"
     CDS             12135..14726
                     /gene="mprF"
                     /locus_tag="U2K71_002083"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysylphosphatidylglycerol
                     flippase/synthetase MprF"
                     /protein_id="HEM7316116.1"
                     /translation="MLKNTIQWLKNHLGLFKTIFLISVIVIIVGELMSIGKTLSIQQL
                     GETFATIPVWKSGLMLLIGLISVLPMVGYDIILNRMLEQKQNPRYLFETSWLINTINN
                     IAGFGGFVSVGLRSELYGQKKESRNVIQALSKVFLFLMAGLSIYSLLSFLLVVFTPVA
                     PFLKQYWIWLIGGGLYFPVVFLFTTFKKKGFLGGTSLKTQGQLLLVSLLEWTGVLFSF
                     LSVGYLMEIRIDLWQTIPLFIAASVIGIVSMIPGEIGSFDVMMIIGLSAIGVPREIVV
                     VWILLYRLFYYIIPFLIGIVFFFKNIGSTFDQRYSGIPKQLATEIAHKIVVVLLYFSG
                     IMLVLSATIPQAFTEFRWLHSLNPLKFHFIIQFPSILLGFLLIIMGRGIAARVKRAYL
                     PTIFLIVLALLYVLLSDFSFTPVVFLSILLLIILASKNELFREQLIYSWEWRTIDGII
                     IGALSLLYIVIGVYNLPDFPHRRHHFISFFLFPSEKIWFSGLLAIIAVSFMIVLFVHF
                     LQGEKKQIGEAFNEEKALKILTTYGGNSDSQLIFLKDKRMFAYEKDGEPTVLLQFACF
                     NNKCIVMGDPSGKKEDFPEAIEAFIEETDRLCYLPVFYETSEEIVMILHEFGYDFIKM
                     GEEAYVDLNSFTTSGKKMKGTRAVLNRIEREGFTFDVLQPPFSTEQMSTFKNISDNWL
                     GSRKEKGFSLGFFSEDYLQRAPIAVVKNTQEEIVAFATIMPTYTNNKVGTIDLMRYDS
                     KKAPSGSMDFLFLHLFAYMKEEHIQWFNLGMAPLSNVGTSRKSFLQERIAYLVYEFGS
                     HFYSFHGLREYKSKYATNWVSRYTLYSRDSWIAYVMIALLIIDNAPVEQTQGRISGLK
                     KWIRRKY"
     gene            complement(14914..16101)
                     /gene="tuf"
                     /locus_tag="U2K71_002084"
     CDS             complement(14914..16101)
                     /gene="tuf"
                     /locus_tag="U2K71_002084"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Tu"
                     /protein_id="HEM7316117.1"
                     /translation="MAKEHYDRSKPHVNIGTIGHVDHGKTTLTAAITTVLGKKGLANP
                     QDYASIDAAPEERERGITINTAHVEYETEKRHYAHIDAPGHADYVKNMITGAAQMDGA
                     ILVVSATDGPMPQTREHILLSRQVGVKYLIVFLNKVDLVDDEELIDLVEMEVRELLSE
                     YGFPGDDTPVIKGSALKALQGDPDAEAAIMELMDTVDEYIPTPERDTDKPLLLPVEDV
                     FSITGRGTVASGRIDRGAVRVGDEVEIVGIKPETQKAVVTGVEMFRKTLDYGEAGDNV
                     GVLLRGIQRDDIERGQVLAKPGSITPHTKFKAEVYVLTKEEGGRHTPFFNNYRPQFYF
                     RTTDVTGTITLPEDTEMVMPGDNVTIDVDLIHPIAVENGTTFSIREGGRTVGSGIVTE
                     IEA"
     gene            16469..16825
                     /locus_tag="U2K71_002085"
     CDS             16469..16825
                     /locus_tag="U2K71_002085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM7316118.1"
                     /translation="MKKLIGVLVVLGIFLGGSFAAYHYFYGGEAYYTKITTNGEMSAT
                     QADNGEKFTTYTYKQAAYNKDGEKKQVTLREEREHPLKMNAYLKLKVNPRKGVLSWEE
                     IKASEVPKNAAEKIDN"
     gene            complement(17066..18070)
                     /locus_tag="U2K71_002086"
     CDS             complement(17066..18070)
                     /locus_tag="U2K71_002086"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoid-associated protein"
                     /protein_id="HEM7316119.1"
                     /translation="MDIYLKKAILHIIDRETGSPVFSQKELDLTKEYIRDFLQKKIQK
                     ISSAQTKTGQLAEESMIVESLKIAQENFVEASEAIVQRWFDIYQESEEAPSADVFVVL
                     YELDTVMYLSLLKVNYREAYTHFVEADEAGIDNRLILNRAILGAKTQKADEAFAVNLT
                     DWTYELIEKKYEFSGKKELYFSGRVIESVPAPSLEENVKVIQKVAKHLGKKFETEEFD
                     IMADLKEAVYDTIEEKGRLDHEMIAEKVFKENITAKMAFKEEVQEQGFVPEAPPVKEV
                     QEISEKKFGKQKFKLSNGIELIVPVDVYRNPDLIEFVNNPDGTISVMIKNVDEVLNRL
                     "
     gene            18236..19087
                     /locus_tag="U2K71_002087"
     CDS             18236..19087
                     /locus_tag="U2K71_002087"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HEM7316120.1"
                     /translation="MNSLTSTYRLSNGYEIPVVGFGTWQTPDGDVAVSSVKEALAAGY
                     RHIDTAQGYKNEESVGQAIKESGIPREEIFLTTKLWNANHSYELVMSSFEESLKKLQT
                     DYLDLFLIHWPNPVAFRDNWEQANADTWRAFEELYEAGKIKAIGVSNFLPHHLDTLAK
                     TAKIMPMVNQVFLAPGELQTTVVEYAKKHDMILEAYSPLGTGKIFDVPEMKQIAEAHD
                     KTIAQVALRWSLQHEFLPLPKSVTPSRIKENTELFDFELTEEEMKQIDQLDGVVGKAK
                     DPDTTQF"
     gene            19104..19349
                     /locus_tag="U2K71_002088"
     CDS             19104..19349
                     /locus_tag="U2K71_002088"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316121.1"
                     /translation="MDPIERLNSLSEEVTQTFHSDFVFLIDAEKIQHFPARNWTHDQI
                     IEELKKRFDHSLMVKPWHEHEVIYSPELPVFALIPKK"
     gene            19536..20240
                     /locus_tag="U2K71_002089"
     CDS             19536..20240
                     /locus_tag="U2K71_002089"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316122.1"
                     /translation="MKKKQKQKLLNQFRPSLDGVRGQLFRSLEEESLRRYGLPLQVML
                     DPKNRQILTIGLAGKTNEDTRINVPIDDNFNTVLKRIRSGENGIFERFRDNLLIEIVS
                     YWNDQRLKNDEPTAQAVATPVTEETVSVPEEKTEKPKADTTENTRSTDTNAALSFADF
                     SKAVAEYPKFYTEQNEHEVVINEKNADEPRKLATISMTTENEFVIEKALERKYKVKLT
                     LIPLIEQFAATAITDR"
BASE COUNT         5808 a         4204 c         3586 g         6908 t
ORIGIN      
        1 aaaagccgtc tagacactca gacggactgg tttctttttt ctttttcata gcattcctga
       61 cagattccat ggaacgttag ccgatgatct tttactaaaa aatgataacg ttcttctact
      121 acttgctcca cttcaagtaa aagatcctct tctacttcct cgatcgtgcc gcagttcaag
      181 catagtaaat gatgatggaa atgtttggcg ccttcttttc tcaaatcata acgggctaat
      241 ccatcattaa aacttacttt atcgacaatt ttcaattctg tcaagatttc aagggttcga
      301 taaacagtag caagtccaat ttccggactc ttttttttca ctaaaaagaa gatttcttct
      361 gcagataaat gctctttctc attttccaaa agcacaagca gagtagcttc tctttgcggt
      421 gtcagcttaa accccgcttc atgtagctgc tgttttgttt ttttcatcgc cgaaatagca
      481 ttcatgacca tttactcaac cccaacaatt tataataatt acaaataaaa aaagatataa
      541 cactatgtta attaaaatta ttataagcta gttaagaaaa acatgcaagg gattctcaat
      601 tagatttttt gactagtttt gtcacaccgt tttcttgtgt aatcaatcct tgtttcaaaa
      661 gattacctaa tgcgcgctta aatgctcctt tgctgatacc aaaagctttc atgatttctt
      721 ctggatccga tttatctgta aagttgattt gctgatctgc agaacgttct aaaatggtta
      781 aaatcatttg tgcatcatct gatatagctt catatgctct tggcttcaat gaaagattca
      841 gtacaccgtc tggtcggaca ccaatcactc gaccttttat ctgctcgcct agacgaggtt
      901 cattatatcg ttcagaagga tggataaacc caagatagaa atcttccgtt aaaacgtacg
      961 tacctgttaa tttcaagcgg tatgctgttc ctatcacatc tttgtttttc atttcttctg
     1021 ttccatattt gcttaaagat ttgaatattt tttcatctgc taaactgccc caaatacggt
     1081 cttttgcatc aactctaagg gaaatcatca agcgatcccc ttttttaggc catagctctg
     1141 tcattgtcgg caattcatca agagatacga caaagtcttt atctggaaga ccgatatcga
     1201 caaacgcacc taaatctttt cgtgtagcag taacgattcc aaaagcgtaa tgaccgatac
     1261 ggcttttagg aatttgtgta gtgatccgat tttcatgttt ttgattttgg taaccgaatc
     1321 cttcaactgc ttcaccgatt ttgtgttcac cttctgattt gcttaagcca aaggtttgtc
     1381 cgtttttttg gacaaagtag tttttttcat tttcatcaat aataatacct gtaaatatag
     1441 ttgctaataa atcgttcatt tttttctccc tctatagtga aaggttttct taatacattt
     1501 ctttgctatc ataacataat gttatctact tgaatagctg agccttggtt tggaaaggtg
     1561 aaaaaaggag tatgacaaca gttgtgtcat actccccttt ttcgaataaa cggtgttaaa
     1621 cggcaggctc acagcctctt tcatttgcct aaaattaatt gctcccattt cctccgaacc
     1681 atccagtttt acagttgatg gttacgatgt aaaatggcaa agcactttct ccaatgcctt
     1741 tcatcaatgc agattttgtt gaagtggcat atagattata atagccttcc ccattttcga
     1801 ttctggcttt ttctaatctg taacctcctt caacgatata tccacgttgg atacatgtag
     1861 caatcacttt gctttggaca cctggtgccc acgtccatgc tggattagat gcatcggcaa
     1921 tatcacttgc tctggcagat ttgattggct gattcgctga gtttgaagag gaaggggtcg
     1981 aggatgccgc ttcttgctgt gctgcttgtg cgaccgcagc ttgttgagcg gcttgagctg
     2041 cagctgctgc ttgagcggct tgttttgctt gttcttcagc tgcttttttc gcttcttctg
     2101 cttttttctt ttcctcttct tcttttttct ctttatcagt gagcatctta tctacttgtt
     2161 tcaattgttc attcaactga gcagatagat ctgtattttt cactttttct actgcttctt
     2221 ttgccgtctt gtattggtcg cgtgttgctg aatccactac ttttccgtct ttaaagacta
     2281 tagctgtttt ttctttagct gtttgcaatt cagcatattg atttttagct tctgtttgtg
     2341 cttgttgaat caattttcca aaatctgtgt tatccgtttc gattgcttgg atctctttat
     2401 ctgctttcaa cttgatgtca tctgctagat gatcatcgac gatggcaggt gctatgaacc
     2461 actcattcac tttttggata tctcggatct tagattggat atcttggtaa gttgtttcta
     2521 actctgaata tcctttttca ttcttaagac tggataaatc attttttagt tttgataaat
     2581 cttgatgaat catatctgca cgaataaatt gatggtgatc atctgtataa aaggcagcaa
     2641 gtttctcatg aagttcttcc atttgttgct gtctttttgc ttctgctgct acttcggctt
     2701 ctactttttg atgatggtca taataagccc agcttcctcc accgagtaga agtactgctg
     2761 ctaaaacgag ataaggtact ttttttgatt ttttcttttg cgctttatct ggatctgttt
     2821 tttctaagtt tgattcttga tgtgtttttt cagtatctgg cacgattgtt attgatttcg
     2881 cttctgcttc aaacagctta ggctggttct cttctttttc tatttttgct tcaagatcag
     2941 taccctcttt gcttacagat tgattttcag catcggattc actatttatt tcatcgggct
     3001 ctgttgactt tttgtctgtg ttttcgagta tttctttttt ctctgcttcc ggcagtttat
     3061 tcgttttttc cttttctaat tgtttttcag taaatacttc agttttttct gtgatatccg
     3121 tttttacgtc ttttattttt actgtttctt tacctgtggc gttttctcta tcagagtctt
     3181 tttcagtttc ttgttccttt tctggctcta ctttgttttc ctgtgcaatt ttttcactga
     3241 tcattttgag cagctctttc gcgtgctctt cttttgtatc ttcacgatgt gctttgatat
     3301 aagcggatag tatcgaattt tcttcctcat tatcgacgat ttctttcagt tcagctactc
     3361 ttcttttttg ttttgattca agcgtttctt cttgattctt tcctgattct tccgaagtct
     3421 catgaacagt tgctttcttc tgttcatttt tctctatatt agttacttga tcgtgagcag
     3481 gtggttcttc gtcttctggt tctgcttttt cgaataattc cattacagat tctagaggta
     3541 aatctttcaa ttccgaccat tcgattggat cgtttaattc tgtatcagga aagactgcat
     3601 cttcagttga tttatcctgc atagctgcat ctgtatttgt ttcttcaatt gattctttta
     3661 tttgctgatc ttctgcagtt atttgatgac cgcagtctgg acagatagta ttctgctcca
     3721 tttttttcga tccgcaattt ggacattttt tcatcactta gcgatcggct cctttactag
     3781 gtttacgcta ccatttaatc tatcatattc tatacgtcac gccaatagct tatttttagg
     3841 atttacttaa aaataatgaa tgaatttcct tcctatagaa aaaaaagctt gagactgcgt
     3901 agtctcaagc tttttttctt ttaaaataaa ccaaacagtt ttactgattt ctcttctggt
     3961 caatagtcca cgcttattct aagaaatctt ttaattgttt tgaacgagaa ggatggcgca
     4021 attttcttaa tgctttagcc tcgatctgac gaatacgttc ccgagtaaca ccaaatactt
     4081 tccctacttc ttccaatgtt ctcgtccggc catcatctaa accaaaacgt aagcgaagga
     4141 cattctcttc ccgatcagtc aatgtatcta gtacatcttc caattgttct tttagtaact
     4201 cataagctgc gtgttctgct ggacttgttg cttcctggtc ttctatgaaa tcacctaagt
     4261 gagagtcatc ttcttcaccg attggcgttt caagggaaac aggttcttgc gcgattttca
     4321 agatttctcg gactttttct gttggaagat ccatttccgc accgatttct tctggcgtag
     4381 gttctcttcc aaggtcttgc agcaattgac gttggatacg tatcaattta ttaattgttt
     4441 caaccatatg aactggaata cggatcgtac gtgcttggtc agcgattgct cgagtgatgg
     4501 cttgacgaat ccaccatgta gcataggttg aaaacttaaa tcctttgcgg tagtcaaatt
     4561 tttcaactgc cttcatcagt cccatgtttc cttcttggat caagtcaagg aactgcatgc
     4621 ctcgtcctac ataacgttta gcgatactta ctactaaacg aaggtttgct tctgccagac
     4681 gctgtttcgc ttcttggtcg ccttcttcga ttttcaatgc taacgctact tcttcttccg
     4741 cagttaaaag ggagacacgg ccaatttctt tcagatacat acgaaccggg tcattgattt
     4801 ttacaccggt tggcgcagaa agatcttctg tttttgcttg ttcagctttt ttctcagcac
     4861 tcttcaagct gtgaattgat ggatcaccat tttcatccac cacactgata cctgcgtctt
     4921 caatcttttg gatcaatttg tccatttcat ctgcattcaa agtgaatggt gtagcaagct
     4981 gattggataa atcatcatat acaacttgtc gtttgggttt gttttctttg atgaaagctg
     5041 ctactgctgc ttcatacgtt tttttatctg ttccatttgc cataaaagaa ggcccccctt
     5101 gagtagctat ttggcttgtt ttaactgttt cgttaaattg atgatttcaa tcgtcaattc
     5161 aagctcaagc tgtttgtttc cagttcgtct ggcttcttgc tgcatttgtt tttttaatgt
     5221 tatttcatca gctaagccag atttgctgat cacatctagc aagtcttgga tttctcgtgg
     5281 cgaactttct tctgccaccg gtaaatattc gatttccacc accaaccgcc ttaaattttc
     5341 atcttgcaaa aagtctaaaa actctgcaag gttgaattca taatgttgtg agacatatgc
     5401 atcgaataaa aaataaactt ctgcatatgc atcatgaata aactgaaatt ctcgttcttt
     5461 taaaatatta cggacagaaa cttctttaaa tgcacgatat aatagaatct gctctgcctt
     5521 ttctacttgc gtcaaaggtc tctttttccg tgcagtttct tgtctcgtaa cctgagtgtg
     5581 ttgactttcc tgaggttctt gacgattttg ctgatttgtc cttcgaagat cgcgcagttg
     5641 tttttgtatc gtctctctgc cgatctgaaa ctctgttgac agttgagata gatacatatc
     5701 ttgttcgatt ggtgaaacaa ctcggctaag ttcaactagt aattcttcga ggtattcgat
     5761 cttgtctttt tcattatcta aatttttccc ttgttttaaa taacgtgatt taaaagtaaa
     5821 gactgtttcg cgcccatgag ccactaattc ttggaaagat gcttctccat acttacggag
     5881 atattcatca ggatcaagct tttctggaac gactacgaca gaaagtgcta atcgactgtt
     5941 ttgccgaagc aattcaatcc ctcgattggt tgcttcaaaa cctgcgttgt ctccgtcata
     6001 gcaaaagacc aattctttcg tcagcttttc catttgttgg atctgttgag ctgttagact
     6061 tgtccccata gaagctaatc cagttttgat accagcctgc catgcagcaa tcacatccat
     6121 aaacccttcg aacaacaaaa tttctccgtt tttccggata tcgcttcttg ctttatcaag
     6181 attaaataaa acttgtcttt tattgaacag ttctgtttct ggactattca agtatttggg
     6241 ctgatctttt tcctgccctt tttcaggtgc aagccagcgt cctgaaaaac cgatcgtctg
     6301 gccttgagaa ttacgaagtg gaaacatcac tctttgataa aaacgatcag acatacggcc
     6361 atcatcatgc gtgacaaaca atccagagcg ttctaacaat tcatttgaaa ttgattcatt
     6421 cttgaatact tgctgaagaa aagaacgctc attaggtgca aaacctatgt tgaattcttc
     6481 aattaattct aaagacaacc cacgactttg caaatattct aaagctggct gtccagcttt
     6541 tgtatgcaat agcatgtgat gatagatttc cgctgctttt tcatgagcag caatcagttt
     6601 ccccgtagca gattcaggat tttggacagc tatagtacgc tgacgccatt gatcttctaa
     6661 cggtatctgt tcgaagtctg ccacttttac cactgcttct ggaaaactta gaccttctag
     6721 ttcttgaaga aaagtaaaca cgtttccgcc tttaccacaa ccaaagcaat gaaaaatctg
     6781 cttgtcttct gcaacagaaa aagacggtgt acgttcttca tgaaaaggac acagccccaa
     6841 gtaattttta ccagactttt tcaactggac atattgtcca atcacctcaa cgatattcgt
     6901 ttgactgcgt actgtttcga taacctcttg aggaatccgt tgagccattt tttcaccccc
     6961 tgctctattg atatcttttg ggtttaacag aagatatttc aggagaataa ccggtaaaaa
     7021 atcgcactaa ctgaaatcag tgcgatttta aaggacacaa ttatacatgt ttgattgtat
     7081 catatttcat tgctttttca agtataacac atttctatct cattaagcat caatttgcta
     7141 ctcttttcag taactgattt tgaatatcag ccttccagta caatactcga agaacaatat
     7201 ttacgagcat gaatacaaag aagtatccta cgctctctcc caaccctaag tttacttcca
     7261 aaattccatg gaacagcatt gcagctacat atatccctac gatcgtcgtt actgctagga
     7321 agatgcgcaa tgggttgaag ggataacaag cttttacaac tgccatgcaa ctgatcccaa
     7381 tcagcaagta atacatcaaa gtagtagttt cagcatttcc ccatgcgaat gtttttccaa
     7441 gtagatacac cgcaatgata ttcactacta caagcaaggc atttggtaaa gcattaataa
     7501 gtgctgtagg caaataacgg taagtgattt ttcttttatc ttgttcaaaa gataaaaaga
     7561 aggaaggata cccttcaatc gctaaatcaa tcagcgtgat ttggataggg ataaacggaa
     7621 atccaattgc tgtaacggca catataatcg ataaaataaa tgaataaatc gttttgataa
     7681 aaaagatgcc tgagactttc gtcacattgt tgaccactcg gcgtccttca aacaggactt
     7741 ctggcaatgt agtaaaatca gagtccaaca ataccagatt cgcaatctga cgtgttgctc
     7801 catcaccttc tgccatagca atgctgcaat ctgcttctct taacgccaaa acatcattca
     7861 caccgtcgcc tgtcattgca accgttctgc cgctttcttt caattcattt accagcaatt
     7921 tcttttgctg cggactcact cgtccaaaaa ccgtatagcg atgagcagct tctcgcactt
     7981 ccatttcttc agtaatttga gaaagatcga catacgcatc ataattcggt aatcctgcac
     8041 gtctagcgat attggagaca gtaaaaggat tgtctccaga aatcactttc agatccacac
     8101 cttcctcatg aagataagct agtgtttctt gagcgttttt tcggattgga tcatcgattt
     8161 caaacagagc aaccggctca acaaatgctg gtttttcttc gttcaattgc tgatttggag
     8221 cgatccccaa cataagtacg cgatagccat tcgcttgggc tgtttttaga ctgtccggaa
     8281 gccgatcttc ttccaccaaa cgttcaggcg ctcctacgta cacgactccc agttctggaa
     8341 attccatcgc gccccatttg cgatcagaag aaaatgcgac tttgtttgtc acatcatacc
     8401 gattagaaag ctcaaaataa tctctcatag cccgcatagt gatgttgtta tcactgcttt
     8461 ctgccagata cgtccccata atttctggga tggctttctc gtacagcgga tgtagtgctt
     8521 ctacccgctg gatactcatt ttcccttcag taatggtccc tgttttatcc aagcataatg
     8581 tatcgacatg ggccagcgtt tcaattgaat acatgtcttg aacaaggatt cgtttttttg
     8641 ccagtttggt tacccctgtc gttaatgcaa tacttattag caataccaaa ccctttggaa
     8701 gcatgcctaa caaagctgct gcagatgcaa taacagaaat tttgatagaa gcatcgcgga
     8761 tgaacagtgc ttccaaaaat agtatgatcc ccaaaggaat aatcacgaaa ctagtgaatt
     8821 tggatacttt gcgaatcgaa tctacaagtt cagaacgaat agctttgtga gtctttgcct
     8881 cgctagtcaa gcggacagca taattatctg cacctactcg gataacttca gccatgacag
     8941 aaccgcttgt caaaaagctt ccagaaagca attcagctcc agtttctttg acaatcaaat
     9001 cagattcgcc tgtcaaaaga gcttcatttg cttctgcccg tccagatata acctgtaaat
     9061 cggctggtat ctgttctcct gctccgattt ttaccaaatc tcctaatacc agctctttag
     9121 tagaaatcga ttgttcttta ccgtcccgga tcactagtac ttctgattgg gaaacaatcg
     9181 ataatttccg tacaagattt cttgcatgta tttcttgata aattccaata acgatattga
     9241 cgacaataat tgctaaatac gccatgttgg aatacgcacc tacaaataac agacaaatcc
     9301 caataccaaa gttcagaaaa ttgaataacg tcaatatatt atcgctgaat atttgttttg
     9361 tactttttga tgcatcttcc tggtagtcat tttgctggcc agaagccatt ttctgaacta
     9421 cctcttgctg tgtaagtcca ttcattttct ttttctccat tttccgccaa ctttcaaaaa
     9481 taatcacgtg tttaagtcta gcgtgttcca agattaaaaa caacttgttt ttataaaatt
     9541 tatagaaaaa cacacgaatg attttacaaa aaagatgcca acctttttgt ttgaatcacc
     9601 cgtgtgctgt ttttattatc aatctatctt tacttatatt gttcctccaa acggctcatc
     9661 caccaaccta gtgctgtacc ggctataaaa agaacaggac aagctagtac cccagcaact
     9721 cccaatcccg cgtaatcggt aggaatgatc ataatcgttg aagcaaaaac tacacctaaa
     9781 atgaaatgga atagcttgct atatgccttt gagaaaatgt aatccatgat ttttgacaat
     9841 ccaagaacac aaagaatccc accaattccg atcggcacaa tcacttcaag tcgtacttct
     9901 ttaaatccgt cagccatcgc tttatacaat cccatataca ccaaaaaatt agaagggctc
     9961 aatcctggga cgatcatacc caaaccaatc aatccgccgg caatcagcca tgttccaaaa
    10021 ttctgatcga cttgcccgtc aaataattga gcgccgtata ataaaaacaa ataagccaga
    10081 acaaaagtaa caaccatgat gatcacatca gaagtatctc ttcctttttt ccctgcttct
    10141 ttccataacg caggaacagt tccagcgata catccgacaa aaaaccataa tatcgttgtt
    10201 tcatacgcac ctaataaaaa actgactaaa aaagaaagta gaaagatgcc tgtcactccg
    10261 cctagaccaa caggcagaaa aaacaaaaca ttttctttga aattttttgt aaggtgggct
    10321 aaaaaagaaa tcagccgctc atacatgcca aagacagctg ctaatgcacc accgctgact
    10381 cctggcaaga taaatcctga accgataaac attcccttca caaaccgtaa catccagtct
    10441 ttggttgtgt accccacttc ttgattttcc attttttgcc ttttttccac tatttgcgta
    10501 ctccttcttt catttaattt tattcatgat tgaacatttc ctgctatttt acttaaagac
    10561 tttttcatag gtctctatta ttttttcatt cattttgaca atatcaggat aaaacatgtt
    10621 acctccatta tgatagagat agccgccatt gtaaagcaag gactggatcc gccaatatcg
    10681 gtattgtttc ccttcctcat ttcctaaaag tgggctcagc acatctttag aatacgcttc
    10741 tgctaattct actgtgtttt ctccgccatg attttttaca taggcaatat aatctttccc
    10801 aaaattatat gcttggacag ctgtccataa atcgcatcct tgtttctttg cttctactat
    10861 catttccgcc aaatatttga caccttgatc aatactgtca gctggattat caatgatctc
    10921 agcttctcca tgcagacttt ccgagctctg catgagatct gtcccttttc cttttgattc
    10981 ggtcaatatt atcgccttga tcaaattctc gtagttttcc atatgataat tttttgcagc
    11041 tgttgttatt tctatttgat agctttccac tcgttttacc gaacgataag ttcttcctca
    11101 taaaaacaac accgcaccaa taaccgtcag aatgatcatg taaaaaagaa tacggataat
    11161 agataatcgt tttcgcttca tttcatcacc tagttatttt gacatctttt tttatcttac
    11221 tatttcttta tcttcttggc tatttcttcc ttgatttctg tgtacgcctt aagaaaaaat
    11281 gaattttcct gtattttttg acatcttacg aaaatgatcg ttgtttttca gtaaaaacaa
    11341 ttgcccaatg actgatataa tcgttatact ttacacagta ggtgattatg tgaaaaaaga
    11401 aaagaaatat gcaaaaatgc cagatgggag cgaaatttac tatgaaaaga gcggtcaagg
    11461 cttccctctc tttttactgc atggcaatga cggcagcggc cgcttttttt cagaacaggt
    11521 ccctgtactc gaacgctact ataccgtgta tctagtagat agccgaggac atggtcgttc
    11581 gacaaacgaa gcatctatgt tgaattttca gttaatggcc gaggatctga atacgatcat
    11641 gttgctggaa aaaatcgatc aggcagattt tcttggattt agtgatggag caaatctagc
    11701 actggtattt gccagcagct tccctaaaaa agttcatcga ttgattttaa attctggaaa
    11761 cacgcttgtt aaaggcgttc gtttttctgc cagagtcatc agtaatattc attatgcatg
    11821 ggtatggtta ctctctttat ttcgcccaag tcttcgcaaa aatcttttag ttatcaaact
    11881 tttgctgcat gacatcgggc ttacagaaaa tgatttaaaa aaaatcaact cccccacttt
    11941 gatcatcgtg ggaaaaaaag atgtaatcaa gctaaaacat tctttgtata tagctaaaac
    12001 tataccaaaa gcttcctttg tgcttgtgaa agaacaagga catgaattag ctagaaaaga
    12061 tcctgaacgc ttcaatcgag aagttcttca atttctttct gaaacataac tattaggaat
    12121 agaaggtgat ttatttgtta aaaaatacca tacaatggct gaaaaaccac ctaggactgt
    12181 tcaaaacaat ctttttgatt tctgtcattg tcattattgt cggtgaattg atgtccatcg
    12241 gtaaaactct atctatccag caattaggag aaacgtttgc cactatacct gtatggaaaa
    12301 gcggactaat gctattgatt ggtcttatct ctgtgttacc tatggttggt tatgatatta
    12361 ttttgaatcg gatgcttgaa caaaagcaga atcctcgtta cctgtttgag acaagctggc
    12421 tgatcaatac gatcaacaat attgccggtt tcggcgggtt tgttagtgtg gggcttcgct
    12481 ctgaactata tggacaaaag aaagagagca gaaacgtgat ccaagcatta tcgaaagttt
    12541 tcttgttttt gatggctgga ctttctattt acagtctgct ttctttttta cttgtagtct
    12601 tcacgccagt tgctcctttt ttgaagcaat attggatatg gttgatcggc ggcggtctat
    12661 attttcctgt cgtcttttta tttactactt tcaaaaagaa aggattcctt ggtggtacct
    12721 cacttaaaac tcaaggacag ctattgctag tttcattgtt ggaatggacc ggtgtcctgt
    12781 ttagtttcct ttctgttggt tatttaatgg aaatccggat tgatctgtgg caaacaattc
    12841 cattattcat tgctgcttca gtgataggaa tcgtgtctat gattccagga gaaattggga
    12901 gtttcgatgt aatgatgatt atcgggttgt ccgctattgg tgtgccaaga gaaatagtcg
    12961 ttgtgtggat cttgctctat cgcctgttct actacatcat tccgtttttg attggaatcg
    13021 tctttttctt caaaaatatc ggttccactt ttgatcagcg ttattctggt atccctaaac
    13081 aattagcaac agaaattgcg cataaaatcg tggttgtctt attatacttt tctggaatca
    13141 tgctagtact ttctgccact atcccgcaag cttttacaga gtttcgctgg cttcatagtt
    13201 tgaacccatt gaaatttcat tttattatcc aatttcctag tatactattg ggatttcttt
    13261 taatcattat gggcagaggg atcgcagcac gtgtgaaacg agcatatttg cctacaatct
    13321 tcttgattgt tctagcttta ctttatgttt tattaagtga ttttagtttc acaccggtgg
    13381 ttttcttgtc tattctgctt ctgatcattc ttgcttctaa aaacgaatta tttcgggaac
    13441 aattgatcta ttcatgggag tggcgtacga tcgatggaat cattattggt gcactgtcct
    13501 tgctttacat cgttattggg gtatacaatc tgccggattt ccctcatcgg cgtcaccatt
    13561 ttatttcctt tttcttattt ccttctgaaa agatttggtt ttcgggattg ttggccatca
    13621 tcgctgttag tttcatgatc gttctatttg ttcatttcct tcaaggagaa aagaaacaga
    13681 ttggcgaagc ctttaatgaa gaaaaggccc tgaagatcct tacaacttat ggaggaaact
    13741 ccgatagtca attgatcttc ttaaaagata agcggatgtt tgcctatgaa aaagacggtg
    13801 aaccaactgt tcttctccaa ttcgcttgct tcaataataa atgtattgta atgggtgatc
    13861 cttctggtaa gaaagaagat tttccagaag caatcgaagc ttttatcgaa gaaacagatc
    13921 gactatgcta tttaccagtt ttctacgaaa caagcgaaga gattgttatg attttgcatg
    13981 aattcggcta cgatttcatt aagatggggg aagaagctta tgtagacctg aatagtttta
    14041 caacttcagg gaaaaaaatg aagggcactc gtgctgtctt gaatcggatc gaacgagaag
    14101 gatttacatt tgatgtctta caaccaccat tttctactga gcaaatgagt actttcaaaa
    14161 atatctctga taattggttg ggttcacgaa aggaaaaagg gttttcttta ggctttttct
    14221 ctgaagatta tctacaaaga gcgcctattg cagttgtcaa aaatacccaa gaagaaattg
    14281 tcgcttttgc aacgatcatg cctacttaca cgaacaataa agtaggaacg attgatctga
    14341 tgcgatatga ttctaaaaaa gcaccttctg gcagtatgga tttcttgttc ttgcatttgt
    14401 ttgcctatat gaaagaagaa catatccaat ggttcaattt agggatggcc cctctttcaa
    14461 atgtcggaac ttccagaaag agttttttac aggaaaggat tgcatacttg gtttacgagt
    14521 ttggttctca tttttattct ttccatggat taagagaata taaaagcaag tacgctacaa
    14581 actgggtctc acgttatact ctatattcta gagatagttg gattgcatat gttatgattg
    14641 ctttgctgat tattgataat gcgccagttg agcagacaca aggtagaata agtggtttga
    14701 aaaaatggat acgaagaaag tattaaaaac tgcataccga tagatgaaca ctctcatgat
    14761 gaataaaaga gactgaagta aaacgaaagt tcatgcttct acaatctctt cttatacaat
    14821 tgaagaacaa agagcggtcc aaacagaaat cctgtttgga ccgctctttt attttaaaca
    14881 tttttcatta ccaaaatatt cgagtcgcgt ttcttatgct tcgatctctg taacgatacc
    14941 tgaacctact gtccgtccgc cttcacgaat tgagaatgtc gttccgtttt caacagcgat
    15001 tggatggatc aagtcaacat caatcgtcac gttgtcacca ggcatgacca tttctgtatc
    15061 ttctggcaat gtgattgttc ctgtaacatc agttgtgcgg aagtagaact gtggacgata
    15121 gttgttgaag aatggagtat gacgtccacc ttcttctttt gtcaacacgt acacttctgc
    15181 tttgaatttt gtatgtggag taatggaacc tggtttagca agtacttgtc cacgttcgat
    15241 atcgtcacgt tggatccccc gtaacaacac gcctacgtta tccccagctt ccccgtaatc
    15301 taacgtttta cggaacattt ctacacctgt tacaactgct ttttgtgttt caggtttgat
    15361 ccctacgatc tctacctcat caccgacacg aacagcacca cgatcaatac gacctgaggc
    15421 aacagttcct cgacctgtaa ttgagaagac gtcttccact ggcaatagta atggtttatc
    15481 tgtatcacgt tctggtgttg ggatatattc atctactgta tccatcaatt ccataatagc
    15541 agcttcagca tctggatcgc cttgcaatgc ttttaatgcg gaacctttga tcacaggagt
    15601 gtcatcgcct gggaaaccat attcgctcaa taactcgcga acttccattt ctaccaaatc
    15661 gatcaattct tcatcatcga ctaaatcaac tttgttcaag aaaacaatca ggtattttac
    15721 accaacttgg cgtgacaata aaatgtgttc acgtgtttga ggcatcggac cgtcagttgc
    15781 agaaacaact aagattgcac catccatttg tgctgcccct gtaatcatgt ttttcacata
    15841 gtccgcgtgt cctggtgcat caatatgcgc gtagtggcgt ttttctgttt catattctac
    15901 gtgtgcggta ttgatcgtaa tcccacgttc acgttcttct ggagctgcat caatactagc
    15961 atagtcttga ggatttgcta ggcctttttt gcctaatact gtagtgatcg cagcagttaa
    16021 agtagttttc ccatggtcaa cgtgtccaat cgtaccaatg ttaacgtgtg gtttacttct
    16081 gtcataatgt tcttttgcca taatttacaa acctcctaaa taatatagat cactgaaaaa
    16141 tttcagctct caccttttta ttatagtctt tttttgacta aatcaaaaag aatacgtccg
    16201 acttactttt tgtaagattc gaactaagct tattatacgt caatattggt caaagtcaac
    16261 taagagcact cgtctgtctt atacgcgaaa aaagtacctt ttataaataa aaaaatgtgt
    16321 tttttgagaa aaacttcaag atagtcctta actttctttt gaagcaataa gaacaaaatg
    16381 aggaaaagtt taaaattcag ctgttgccaa caactttcaa tagacgaaat cttctatttc
    16441 gttaataata aggaaagagg tgaagtacat gaaaaaatta attggcgtac ttgtcgtttt
    16501 aggcatattt ctcggcggaa gttttgcagc ttatcattat ttttacggcg gcgaagctta
    16561 ttatacaaaa atcacgacga atggcgaaat gtcagctacc caagcagata acggagagaa
    16621 attcacgact tatacctaca agcaagcggc ttataataaa gatggtgaaa aaaaacaggt
    16681 gacgcttcgc gaagaaagag aacatccatt aaagatgaat gcgtatctta aactcaaagt
    16741 caatcctcgt aaaggtgtat taagctggga agaaataaag gcttctgaag tgcctaaaaa
    16801 tgcggcagaa aaaatcgata attagtaaat ggtcttaaaa ttttattaga taaaaagagg
    16861 ctgggacaga agcgtttaac tccaagaaat aagaaggagt tgcttctgct tccgccgttt
    16921 atacgtgttt agagcgtgag acaaaagtgt tctttacttt tgtctcacgc tctttttttg
    16981 ttatattcgt cttttccgat ccttctcttg acacaattca tttaactgat cactggtact
    17041 tgaacaccta atattctagt catttttata aacgattcaa tacttcatcc acatttttga
    17101 tcatcacgga tatcgtacca tccggattat tcacaaattc aatcaaatct gggttgcgat
    17161 atacatctac tggtacgatc agttcgatcc cgtttgatag cttgaatttt tgttttccaa
    17221 attttttctc agaaatttct tgtacttctt ttacaggagg tgcttctggg acaaatcctt
    17281 gctcttgtac ttcttcttta aaagccattt ttgccgtgat gttttccttg aagacttttt
    17341 ccgcaatcat ctcatgatcc aaccgacctt tttcttctat cgtatcatag acagcttctt
    17401 tcaaatcagc catgatatca aattcttctg tctcaaattt ttttccaaga tgcttggcta
    17461 ctttttggat cacttttaca ttttcttcta aagaaggtgc aggaacactt tcgatcactc
    17521 ttccagaaaa ataaagctct tttttgccag aaaactcata ttttttttca attaattcgt
    17581 aggtccaatc agtcaaattc acagcgaatg cttcatctgc tttttgtgtt ttcgccccta
    17641 gaattgctcg attcaaaatc aagcggttgt cgatccctgc ttcgtctgct tcaacaaagt
    17701 gagtgtaagc ctcacgataa ttgactttca ataaagataa gtacatcact gtatctaatt
    17761 catacaagac aacaaataca tctgcactag gtgcttcttc actttcttgg tagatatcga
    17821 accaccgctg aacgattgct tcacttgctt ccacaaaatt ttcttgagcg attttaagtg
    17881 attcaacgat catgctttct tctgctagtt gtccagtttt tgtttgagca gaagagattt
    17941 tttggatctt cttttgaaga aaatcacgaa tatattcttt tgtcagatcc agctcttttt
    18001 gtgaaaagac tggagaacca gtttctctgt caataatatg cagaattgct ttttttaaat
    18061 agatatccat tttctcattc ccttctgtct tagtttacgg aaaaaaaatc aaaaatacca
    18121 gaatcaaaat cattttcttt taaacaaaaa tttcaagagt agaaacaaaa tggattcttt
    18181 ttttcgaaaa atgcgctaca cttatagttg aacgaataaa aaaggagctg aaagtatgaa
    18241 ttcattaaca agcacttatc gtctatcaaa tggttacgaa atcccggtag taggattcgg
    18301 cacatggcaa acacctgatg gggatgtcgc tgtttcctct gttaaagaag cgctggctgc
    18361 aggttatcgc catatcgata ctgcccaagg atacaagaat gaagaaagcg tcggccaagc
    18421 gattaaggaa agtggaattc ctagagaaga gatattcttg acaacaaaat tatggaatgc
    18481 aaaccacagc tatgaacttg tgatgtcttc ttttgaagaa tcattgaaga agctccaaac
    18541 agattattta gatctgtttt tgatccactg gccaaaccct gttgcttttc gtgataattg
    18601 ggaacaagca aatgcagata catggcgagc atttgaagaa ctgtatgaag caggtaaaat
    18661 caaagcaatc ggtgtgagca atttcttacc ccaccatcta gatacactag caaaaacagc
    18721 aaaaattatg ccgatggtca atcaagtttt ccttgcacca ggcgaattac agacaactgt
    18781 agttgagtat gcaaaaaaac atgatatgat cctcgaagca tatagtccat taggcactgg
    18841 taaaatcttc gatgtgcctg aaatgaaaca aatcgctgaa gcacatgaca agacgattgc
    18901 acaagttgct ttgcgttggt cgctgcaaca cgaattcctt cctcttccta agtcagtgac
    18961 accaagcaga atcaaagaaa atactgaatt atttgatttt gaattaacag aagaagaaat
    19021 gaaacaaatc gatcagttag atggtgtagt cggcaaagca aaagacccag atactaccca
    19081 attttaagtc gaaaggtgat tttatggatc caatcgaacg cttgaattct ctctcggaag
    19141 aagttaccca gacttttcat tccgattttg tctttttgat cgatgcagag aaaatccaac
    19201 attttcctgc aagaaactgg acacatgatc aaatcatcga agaactgaaa aaacggttcg
    19261 atcattcgct aatggtcaaa ccctggcacg aacatgaagt catttattca cctgaactac
    19321 ctgtttttgc cttgataccg aaaaaataga aagaatggaa cggattatgt atatatacat
    19381 aatccgttcc attttttatt tttctttcaa aaaacgggaa aatacgcaaa tttttctgtt
    19441 gtaatttgcg tatacgcaag tttttttgca ttactccttt tcatttggct gtttttttct
    19501 tataatcgaa agggtatgag cgaaggagag aacatatgaa gaaaaaacag aaacaaaaat
    19561 tattaaatca attccgtcct tcattggatg gtgtaagagg acaattattc cgttctctag
    19621 aagaagagag cttgcgacgt tatggtttgc cgttacaagt catgcttgat cccaaaaacc
    19681 gtcaaatctt aacgatcgga ttagctggga aaacaaatga agatacacgg atcaacgtgc
    19741 caatcgatga caacttcaat actgtattaa agagaattcg ttcaggagaa aacgggattt
    19801 tcgaacgatt ccgtgacaat cttcttattg aaatcgtgtc ttactggaat gatcaacgat
    19861 tgaaaaacga tgaaccaact gcgcaagctg ttgcgacacc agttacagaa gaaacagtca
    19921 gtgtgccaga agaaaaaacg gaaaaaccaa aagcggacac aactgaaaat acacgctcaa
    19981 ctgatacgaa tgccgcttta agttttgctg atttttcaaa agccgttgct gagtatccta
    20041 aattttacac agaacaaaat gaacatgaag tagttattaa tgaaaaaaat gctgatgaac
    20101 ctcgtaaatt agcaactatt tctatgacta ctgaaaatga gttcgttatc gaaaaagcac
    20161 tcgaacggaa atacaaagtc aaattgactt tgattccttt gatcgaacaa tttgcagcga
    20221 cagcgattac tgatcgataa aaattctatt tgtcataaaa ggctgggatt taaaatccca
    20281 gcctttttag tcatataata atttctacag caagtaaaaa gaaattcctt ctaacatcag
    20341 gctcaaatat cagcttgacc tgtccaacaa atcacctatg atcctacaat gttaggcgac
    20401 ttctcttcta cctgaagata tcttggagtt gcctgatttc ttaagatgcc taaccttaac
    20461 tataaaaaat tctaataata aacatatttt ctgatagaga aaaaag
//
LOCUS       DASHHB010000051        19524 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.053, whole genome shotgun sequence.
ACCESSION   DASHHB010000051 DASHHB010000000
VERSION     DASHHB010000051.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19524)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19524)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19524
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.053"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            153..932
                     /locus_tag="U2K71_002090"
     CDS             153..932
                     /locus_tag="U2K71_002090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316123.1"
                     /translation="MEKPFVKSLTAVAVGAGAIAICLFGYHINNQRQHQQRINYAESA
                     ITNQKDTLTSLSKEVDKLYSTKEKIFLNPEITEETITSLSNKLSSVKLSADDYDIKES
                     ELPKEAVAIQDEKKAIVTQLDSAESKLKIQTAVNDLFTKNVSNWQQAVDDVIIKEKVT
                     SADVARVRENMSFFKDSPWKTVVMQYLGFADIQIAQVTQLDQLFDTMLKDGQVTATAT
                     YDQYLTVLSQIEQIRNEKISAAYATKAQTVAEQMGYSNISY"
     gene            978..1922
                     /locus_tag="U2K71_002091"
     CDS             978..1922
                     /locus_tag="U2K71_002091"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HEM7316124.1"
                     /translation="MARRRRKSRGKKIFLIILAVLIVLVLAIVGVGAKLYLDLSHSIE
                     KTYESVERTQGERTKPVDLDKQESFSVLLMGIDTGDLGRVDQGRSDTMMVATVSPEDK
                     QTTIVSIPRDTYVDIVGRDTKDKINHAYAFGGAAMAMDTVQKYVDIPIDHYVSINMKG
                     LKELVDAVGGIEVNNDLTFSQDGYDFPIGKITLNGDQALSYSRMRYEDPNGDYGRQGR
                     QRKIVEGVAKKVLSLNGVSNYQSVLNALESNMKTDMNFDEMKKIALDYREAFGTIKQD
                     QLQGEGFMQDNISYQKVSDQELDRVKKELKDQMNLENK"
     gene            1938..2717
                     /locus_tag="U2K71_002092"
     CDS             1938..2717
                     /locus_tag="U2K71_002092"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein kinase"
                     /protein_id="HEM7316125.1"
                     /translation="MEETISLKEIFETLRKHLVIIIISMFAGLAISGIVTFFILTPKY
                     SSQAQLIVTLPQSDTANVNDVNTNLQMINTYKDMIVSDLVLNEVKDRLETEDNVKMTA
                     EQIKDAISVNQAENSQMFSIQAISTNAVAAQQIANTTSQVFQENAKDVMNVDKISIIS
                     GAVANTTPVSPNNKLNLMTGLMLGMMVGVGLAFLLELLDRTVKDEKYVTDTLGFPILG
                     TVPEMSAKELNAGIRKKGPAQTQPKATNDKTAPSRRSRSRV"
     gene            2729..3427
                     /locus_tag="U2K71_002093"
     CDS             2729..3427
                     /locus_tag="U2K71_002093"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpsD/CapB family tyrosine-protein kinase"
                     /protein_id="HEM7316126.1"
                     /translation="MARTQKQKAAQTHAVSLVTLTNPSSPIAEQYRTIRTNIQFASAA
                     GQQIKTIVVTSSGPGEGKSTTAANIAVVFAKSGQRVLLVDADLRKPVIYKTFKLNNAS
                     GLSTVLSSSGSVADEIQRTPVDNLSILPSGPKPPNPSELLSSPRMDQILAEARQLFDV
                     VIFDMPPVVAVTDAQIMSSKTDGTLLVVRENTSRKESLTKAKELLDMVQARVLGVVYN
                     GAEHSKDAGYYYGN"
     gene            3455..4219
                     /locus_tag="U2K71_002094"
     CDS             3455..4219
                     /locus_tag="U2K71_002094"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738108.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine protein phosphatase"
                     /protein_id="HEM7316127.1"
                     /translation="MIDLHCHILPGIDDGAENLEASIAMAEKAIQQGITHILCTPHHN
                     NGKYSNEKSQVISLVASLQAELEKRQLPLTLLEGQEVRITGTLIEDIHRDEILFTDLD
                     DTYLLIEFPTLEVPLYAERLFFELLELGKIPVIVHPERNAHFREDPNHLLPFLDMGCL
                     AQLTAPSYVGVFGKDIQKTAKTMVKHNLVQMAASDAHGVSKRTFYLKECYEQIAKEFG
                     KEKVVQMKQVAKDLINGDEINYPIYEEVKKKKFGLF"
     gene            4263..5714
                     /locus_tag="U2K71_002095"
     CDS             4263..5714
                     /locus_tag="U2K71_002095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HEM7316128.1"
                     /translation="MYRKNSSGWSKHIDFILLDLFCVQLAFYISYVMRQGDWNPYVIP
                     IYRHMAIFIELENLTIIFLFEAYKNVLKRGYYKEAVASIKQSFMLLLVCSLYLITMQD
                     GNDYSRVSLFIMGVVYGLLTYIIRILWKGLLHHRMKNGEDVSLVIVTSQKIAKNVVRN
                     LKEKNYGMYKLSGVIVVDRDMYGTTIGGVPVVANADNAAEYLLQNWVDEVFINVDESV
                     PYPKELIARCSEMGLAVHMNLTKVSDSTRGKQFIGRVGDYTVITSSINVMTMRQAFIK
                     RTIDILAGIIGCIATGVIFIFVAPVIYIKSPGPIFFAQERIGQNGKTFKMYKFRSMYM
                     DAEERKAELMKENKMSNNLMFKMDFDPRIIGNKILPDGTKKTGIGQFIRSTSLDEFPQ
                     FYNVLMGKMSLVGFRPCLKSEYEKYDFHHRARIAMKPGITGMWQVSGRSDITDFEEVV
                     KLDAEYIRNWSMGLDFRILFRTVKTVLHRNGSM"
     gene            5746..6882
                     /locus_tag="U2K71_002096"
     CDS             5746..6882
                     /locus_tag="U2K71_002096"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HEM7316129.1"
                     /translation="MARKKLKICMLGHKRIPSREGGIEIVVEELATRMVALGHEVTCY
                     NRSGHHVSGKEFDQHKNKEYKGVKLKTIFTLNIKGIAAMSSSVFGGIRAAFGKYDIVH
                     FHAEGPCAMLWLPKLFGKRCVATIHGLDHQREKWNKLASTYIMLGEKCAVKFADEIIV
                     LSESVQNYFEDIYGRKTRFIPNGVKKIEIKSAGLITEKYGLTKDSYILFLGRLVPEKG
                     IRYLIEAFKDVQTDKKLIIAGGSSDTDEFANELKELAKGDERIIFTGFVQGQELEELY
                     SNAYIYTLPSDLEGMPLSLLEAMSYGNCCIVSNISECTEVVEDKAMIFKKSDVSDLKI
                     RLEEACNQSEMVKVLKNQATEFICSKYNWDKIVQETLNLYRGNI"
     gene            6882..7988
                     /locus_tag="U2K71_002097"
     CDS             6882..7988
                     /locus_tag="U2K71_002097"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HEM7316130.1"
                     /translation="MIRILQCVNDMHRAGLETMLMNYYRNIDRTKIQFDFLTHRPYKS
                     DYDEEIISLGGKVYYAPRLYPQNYPKYFKWMEQFFKDHPEYEIVHSHIDAMSYLPLKA
                     AKKAGIPIRIAHSHNTSIDRDFKYLLKQYYRCKLTSVLTHEFSCGKVAGDFLFRNDKF
                     TLIPNAVDAKKFYYDESIRNDVRMKLGISSQTFVMGHIGRISYSKNHRFLIEIFKEFH
                     AIKNNSVLLIIGTGDMEEEIKNYAKKSGIDDDIKFLGNRNDIEKFYQAFDVLMLPSLF
                     EGVPLVGIEAQFADLPCFFSEKVPTEVAFSDKTNFISLNQSAKEWAREIANVDISHRD
                     SERSILIKADYNIQTAYKILESKYYELFELIQRG"
     gene            7994..9016
                     /locus_tag="U2K71_002098"
     CDS             7994..9016
                     /locus_tag="U2K71_002098"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="capsular polysaccharide synthesis protein"
                     /protein_id="HEM7316131.1"
                     /translation="MTQKHSSIKRIIHRISEELNATRRIAKITSWKEAFATLQAKIDI
                     QVMNRNGYNESKKQKEHLLKKHEVMLKYYEKTFSDFLKTYDYSQNQIINQSKSPYSNC
                     IWVCWWQGLDQSPEIVKACVKSIKRNAGKHTVIILTEDNYRQYVDIPKWVEEKKNKGI
                     ITRTNYSDLLRLSLLAKHGGLWLDATFYCTKPIPDEYFDIDIWSIRRPDYAHASVASG
                     YFAGYSLACNQESRWMFAIIRDFFLNYWKNNDMMVDYLMIDYMIVLAQRINHEIAQTF
                     DKIPSNNPLCDELIKNLNNIYDEKVWNLLKKDTLLFKLTWKQSFKKQKDDKVTFYGML
                     VENKLL"
     gene            9038..10093
                     /locus_tag="U2K71_002099"
     CDS             9038..10093
                     /locus_tag="U2K71_002099"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM7316132.1"
                     /translation="MKHKVSVIVPIYNVEKYLRKCVDSLLSQTLKEIEIVLVDDGSPD
                     ASGEIADEYQKKYSNVKTIHRENGGLGPARNTGIENATGEYVAFLDSDDWVESDMYEK
                     LYLVASKENADIVVSGHCDFANDKAMVKKQHPLSGNVYDTPNSIKNVRKNLFGHSPKD
                     QEVEAFPMSVCMSLYNKNLLDKYRLRFEKILSEDTIFNLNAYDVANKIAFTEYTDYCY
                     RKEEQESITKSLSPNKQTEFKEFLITLKAIASKEDLECQIRAKRMAIDYCRLYVGLVD
                     DANLGIKEKRKYIKKFIEDEGLIQCWKGYPTNTLPFQQKFFHKMLECKCYTILLLLSR
                     IRKIIKNMTRKKLFIIL"
     gene            10711..11946
                     /locus_tag="U2K71_002100"
     CDS             10711..11946
                     /locus_tag="U2K71_002100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316133.1"
                     /translation="MSVKKMLFMVSTIIVLTSRFWGINTSLSMRYFIMAIWDLYFMLS
                     YFSYREKTYKKYVIKNNFWLTIMPFVIFSIYTLIIWGIGSNAEFENFIKLCSTLLYLI
                     LAYGYACTAFYLFKAEGIDFIFWSGVISYMSGSVFYLIVSYGLNGLISYIKSLITGET
                     NTANFAMEVHDLTFAMGVFFLYYVFFENKNTKKHKIKIIVSLILIFMGLKRIELLALS
                     GAILVYYVLLKWGKKIQIRALVCTICATVISLVFIYIIDTGILALLMSNLGITDDGAR
                     LSFYLYSSKFYELGLGYVGTGWTWFSKYFFNLYLSHFRLNGGRIAASLHSDILTIYIE
                     VGCIVFIIWMFWLFFVKPIRLTKKFGLKTGECVLLLTIYMFILYLTDNTLTYPDTQMI
                     FLLVPMIVSWVDNQQRISE"
     gene            12030..13118
                     /locus_tag="U2K71_002101"
     CDS             12030..13118
                     /locus_tag="U2K71_002101"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316134.1"
                     /translation="MKKILVLANTYYQIIVAIQMKNTIFSDEYVEIIISDHSKNTKEI
                     AKKIEEKKVFDGCHYLESKSFDDNIGYFQKIKDCLQISCGKSNRYAYYLKNCKSEYFD
                     EFICFSMGEVWLEGIYACLYRNNKNVQVSILEEGILSYGVDIIPTARTKAIAVLNYLK
                     KRTPLYNKSYNFYCFFPNIYTGRENTIQIPLVTAGSRTALQLSDIFNPMIDIYKEKKY
                     IFFTSVYDFEGGEPVGEYELVCKIADLVGKDNLIVKTHPRDVRTIYKDNGFIEDKNSS
                     IPWEAIQLSGDFNDKIFMTINSGSVISGSTMSAKPVRTYYMYKLCNIEGNPSCKKNAY
                     DIERLLKQKTMKNILKNVKIAESLDDIL"
     gene            13115..14536
                     /locus_tag="U2K71_002102"
     CDS             13115..14536
                     /locus_tag="U2K71_002102"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HEM7316135.1"
                     /translation="MSKTKYLIKNMGILTISNFSSKILVFLLVPLYTSVLSTTEYGTY
                     DLTISTATLLFPILTLNIVDAVMRFLMDKDKNKKNIASIAIKVIFLSVIVFGSVMYLL
                     NITKLWQDVYGLEKYIFLYYISYVWNQFLIQFAKGLEKVKAMGIAGIISTIVTIIANI
                     FFLLILKWGLIGFFIANILSQIISATYLSVLVRIWEYIDFEAKDPQLKREMLNYSIPL
                     IATVVGWWINSTADRYVVSFIVGVSANGLLSVAYKIPQIINTLQGIFIQAWQISAIKE
                     YGDNETAKFYGNTFVMINLLMCITCSSLIILTRPFSHILYAKDFYEAWKYVPFLLIAN
                     VLNCASGLLGPILSAKKDSKSMMWSAIIGALVNVIMSVVLVYKIGVQGATIATVICSY
                     VIYVVRKRAVGKDIFIKHYHIVVESWGLLFVQAIIEIYFKNYVIEIIIMIILIIINIS
                     YIKQLCYRGRNIISMIKSREHKR"
     gene            14572..15288
                     /locus_tag="U2K71_002103"
     CDS             14572..15288
                     /locus_tag="U2K71_002103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HEM7316136.1"
                     /translation="MVDNYERKNILKKKSIGCRMKIVAIVPMKLNNKRLPQKNTKAFT
                     NGKPLCYYILSTLLKVKQIDKVYVYCSNPDIKEFIPEGVTFLKRSSSLDQDTTSITQV
                     LAGFTKEVEADVYVMTHTTAPFISEKSIEKGLDAVLTGEYDSAFAAKKLQDFLWKDGE
                     PFNYELDNIPRTQDLPPLYEETSGFYIYNRNVMTELHRRIGKKPCIVEVGEIESVDID
                     EAEDFMIADAIYNHLILQGS"
     gene            15307..16890
                     /locus_tag="U2K71_002104"
     CDS             15307..16890
                     /locus_tag="U2K71_002104"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase catalytic domain-containing protein"
                     /protein_id="HEM7316137.1"
                     /translation="MNNVQILDCTLRDGGYCNNWEFGFDNTKKIISGLIESGIEIIEC
                     GFLTNKTVYNKNVTKYTTLEELAQIIPEKREGKLFVAMMNYGEYNIEDLPDYDGSSID
                     GIRIAFHKKNCNEALKLCREIKKKGYLVFVQAMVSLNYTDEEFLAVIKNVNQFKPYAF
                     YIVDSFGMMKGKDLTRLFYMIEHNLEKDIQIGFHSHNNMQLAYSNAQKLITVQTNRSL
                     IIDSSVYGMGRGAGNLNTELFVEYLNDNAGKKYQLKPLLYIIDEILNSFYQKSYWGYS
                     LPNYISAVHNAHPNYASYLSYKNTLTIEAMNEIFEMMDEEKKVSYDKQYIEDLYYKYM
                     ETGKIQEEYMEELRKFLSDKKILLIAPGKSSVDEVERIKEFVTKEEVVIISVNFEYSH
                     IDIDYVFLSNLRRFHELPKESRTKCIVTSNIQASDVYLRTSYKKLLSEREVVRDNAGL
                     MAIRFLADMNVDEIFLAGFDGYSHNEDENYGEQSMEIITKFALLDAMNVQMRDELSEL
                     AKKVKVTFLTDPRWVRIDK"
     gene            17025..17603
                     /locus_tag="U2K71_002105"
     CDS             17025..17603
                     /locus_tag="U2K71_002105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316138.1"
                     /translation="MISILCIVAYHANLRLNYNADGIIVHGSLTANQIFLNYIGIWGQ
                     LGVNCFIFISAYFLSEKRNIHFENVLGIVLECIFYSVLVYGILCACGIETFSLNSFVK
                     EILSFALGRYWFITSYLALYILSPYLNIMLNNLEKLQLQKISMLLFIGVCCYRMLFKT
                     SLFGDCAVLIALYVICYTLKKTIIIFLRDTES"
     gene            17549..18067
                     /locus_tag="U2K71_002106"
     CDS             17549..18067
                     /locus_tag="U2K71_002106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316139.1"
                     /translation="MLYTEKNDNNIFERHRIIGTIVCITVIPLLISIGILFKFIPNAM
                     VNQMVHIGSPLMVIASIFLFYVFKNFSFYSKYINKLARTSLGVYILHENHYFIDGMKS
                     RGLIWDGIFGLGKRFLDRDFWIWFIIAVVVVCIICMAIDVVREKFERQIFKIKSVKNL
                     CKLMNHVINIYP"
     gene            18075..19115
                     /locus_tag="U2K71_002107"
     CDS             18075..19115
                     /locus_tag="U2K71_002107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HEM7316140.1"
                     /translation="MRRKEVKVISFGELLLRLAAPGYSKLFQRDSLETTFCGGEANVA
                     VSLAIFGYQSEFITKLPDSDVGYAAANALRYFGVNVDNIIFGEGRMGLYYLEKGAAQR
                     PSKVIYDRKFSAISLADSDEFKWKEILNDANWFHWTGINPALSENLFETSLDACQAAK
                     KRGITISCDLNYRSKLWTSEEAKEKMEQILPLVDICICNEEDAEKVLGIKAEHTNINS
                     GELNKDGYVQVAREIHKKFGCRYVATTLRRSYSASRNGWSAMLYEAEKDKAYFSTEYN
                     IQIVDRVGGGDSFSAGIIYALCENMSGQKAIEFATASSCLKHTIEGDFNRTTIEDVYN
                     LMNSGGNGRVLR"
     gene            19364..>19524
                     /locus_tag="U2K71_002108"
     CDS             19364..>19524
                     /locus_tag="U2K71_002108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM7316141.1"
                     /translation="MTYTYLTTDELVIIESYLKINQSVAKTAHCLNRSSQTIHKVYLF
                     FKQRKSALE"
BASE COUNT         7123 a         2469 c         3828 g         6104 t
ORIGIN      
        1 agtttgtggt atgattatat agggaaaact tgtttacaaa tatttctgtg aatatattag
       61 tttaattatg tttttttaaa taaaaagctg tcatttttat gtaagtgaat tatatcttaa
      121 tttatctatc atcaatactt aggagtgaat ctatggaaaa gccattcgtc aagtcattga
      181 ctgctgtggc ggtaggtgca ggtgctattg cgatctgttt gtttggatac catatcaata
      241 atcagcggca acatcagcaa cggatcaatt atgcagaatc tgccatcacc aatcaaaaag
      301 acaccttgac ttctttatca aaagaagtag acaagctgta tagtacaaaa gaaaaaattt
      361 ttctaaaccc tgaaataaca gaagaaacaa tcacaagttt gtcaaataag ctatcctctg
      421 tcaaactttc agcagatgat tacgacatca aagaatcaga attgcctaaa gaagctgttg
      481 caatccaaga tgagaagaaa gcaatcgtga cacagttaga tagtgcagaa tcaaaactga
      541 agatccagac agctgtcaac gatttattta ctaaaaatgt atcaaactgg cagcaagctg
      601 tagacgatgt catcatcaaa gaaaaggtga caagtgcaga tgtcgctcgt gtacgtgaaa
      661 atatgagctt ttttaaagat agtccttgga aaacggttgt tatgcaatat ctaggattcg
      721 ctgatattca gatcgcgcaa gtcacgcaac tagatcagtt attcgatacg atgttaaagg
      781 atggtcaagt gactgccacc gctacttacg atcaatattt gacggtgctc agtcaaatag
      841 agcagatccg gaatgaaaaa ataagcgcag catacgcaac aaaagcacaa actgttgctg
      901 agcaaatggg ctatagcaat ataagctatt gacagttaaa acgttgattc aagcaaggtt
      961 agcaaacgga ggaaactatg gcaagacgaa gaagaaaatc aagagggaaa aaaatatttc
     1021 tgattatttt agcagttctt atcgtattag tattagcaat tgtaggagta ggagcaaaat
     1081 tatatctgga tctctcacat tctattgaga aaacttacga atcagtggaa cgaacccaag
     1141 gagaacgaac gaaaccagtt gatctggaca aacaagaatc tttctctgtt ctattgatgg
     1201 ggattgatac tggagattta ggtcgggtcg atcaaggaag atcagatacg atgatggttg
     1261 ccacagtcag tcctgaagac aaacagacaa cgatcgtcag cattcctcgt gatacgtatg
     1321 tcgacatcgt aggtcgtgac acaaaagaca agatcaacca cgcttacgca ttcggcggag
     1381 ctgccatggc gatggatacc gtacaaaaat atgtagacat cccaatcgat cactatgtct
     1441 ctatcaatat gaaagggcta aaggaactcg ttgatgcagt tggtggtatc gaagtcaata
     1501 atgacctcac tttctcacaa gatggctatg actttccaat cggtaaaatt acattgaacg
     1561 gggatcaggc attatcttat tctcgtatgc gttatgaaga cccaaatggt gattacggta
     1621 gacaagggcg ccaaagaaag atcgtagaag gcgtagcgaa aaaagtattg agtctaaacg
     1681 gtgtcagcaa ctatcaatcc gttttgaatg ctctagagtc gaatatgaaa acagacatga
     1741 actttgatga aatgaaaaaa attgctttag attatcggga ggcatttggt acgattaagc
     1801 aagatcagct gcaaggagaa ggcttcatgc aagataatat ctcctatcaa aaagtcagtg
     1861 atcaagaact tgatcgtgtg aaaaaagaat taaaagatca aatgaatcta gagaataaat
     1921 aggaaagtgg agaaaaaatg gaagagacaa tcagcttgaa agaaattttt gaaacattac
     1981 gcaaacactt ggtaataatc atcatctcaa tgtttgctgg cttggccatc tcaggaatcg
     2041 taacattttt catcttgaca ccaaaataca gttcacaagc acaattgatc gtgacactgc
     2101 cacaatcaga cactgcaaat gtcaatgatg tcaatacgaa cctgcaaatg atcaatacgt
     2161 ataaagatat gatcgtcagt gatttagtgc tcaatgaagt caaagatcgt ttagaaacag
     2221 aagacaatgt gaaaatgact gctgaacaaa tcaaagatgc catctcagtt aatcaggcag
     2281 aaaattcaca aatgttctcg attcaggcaa taagtacgaa tgcagtggct gctcaacaga
     2341 tcgctaacac aacttcacaa gttttccaag agaacgcaaa agatgtgatg aatgttgata
     2401 aaatctcgat catttcaggt gcagttgcca atacgacacc tgtttcgcca aataataaat
     2461 tgaatttaat gactggctta atgttaggaa tgatggtagg tgtaggtctg gcattcttat
     2521 tggaattact cgatcggaca gtgaaagatg aaaaatatgt gacagataca ttaggattcc
     2581 caatcttagg aacagttcca gaaatgtcag cgaaagaatt gaatgctggt atccgtaaaa
     2641 aaggaccagc tcagacacaa ccaaaagcta caaacgataa aacagcgcca tcacgcagaa
     2701 gccgctcaag agtctaggag gaaagaaaat ggcacgaaca cagaaacaaa aagcagccca
     2761 aactcatgca gtcagtttgg tcacactcac caatccctct tcaccaatcg ctgaacaata
     2821 tcgtacgatt cggacgaata tccagtttgc ctccgcagct ggtcagcaaa tcaaaacgat
     2881 tgttgtgact tcatcaggac ctggagaagg gaaatcgacc actgcagcaa acattgccgt
     2941 tgtttttgcc aaatctggtc aacgggtgct gctagtcgat gcagacttac gtaagcccgt
     3001 catctataaa acgttcaagt tgaacaacgc aagcggatta agtactgttc ttagttcatc
     3061 aggaagtgta gcagatgaga tccaacgaac accagtcgac aatttgtcga ttctgccaag
     3121 tggacctaaa ccaccaaatc catcagaact actaagttcg ccaagaatgg atcagatttt
     3181 agcagaagca agacaactat ttgatgtagt gatcttcgac atgccgccag tcgttgccgt
     3241 aacagatgca cagatcatgt cttcaaaaac ggatggcacc ttattggtcg tacgggaaaa
     3301 tacgtcacga aaagaatcat tgactaaagc aaaagaactg ttagatatgg tacaagcacg
     3361 agtcttaggt gtcgtataca acggagcaga acacagcaaa gatgctggtt attactacgg
     3421 aaattaagga ataagaagag aggagacaag agaaatgatc gatctacatt gccatatcct
     3481 accaggaatc gatgacgggg cagaaaactt agaagcaagt attgcaatgg ctgaaaaagc
     3541 gattcagcaa ggcattacgc atattttatg cacaccacac cacaataatg gaaaatacag
     3601 caatgaaaaa tcacaagtca tttctctagt cgcttcgtta caagccgaat tagagaaaag
     3661 acagttgcct ttgacgttat tggaaggaca agaagtacgt attaccggaa cgctcatcga
     3721 agacatccac cgagatgaaa ttttatttac agatttagat gatacatatc ttctgatcga
     3781 atttccgaca ctggaagtgc cgttatatgc agaacggctt ttttttgagt tgttagaact
     3841 aggaaagata ccagtgatcg tccatccaga acgcaacgcg cattttcgag aagacccaaa
     3901 tcatttgctt ccgttcctag atatgggatg tttggcccaa cttactgcgc caagttatgt
     3961 tggtgtattt ggcaaagata tccagaaaac agccaaaaca atggtcaaac ataatctcgt
     4021 gcagatggct gcttctgatg cacacggggt ctctaagcgc accttttact taaaagagtg
     4081 ctatgagcag attgccaagg agtttggtaa ggaaaaagta gtacagatga aacaagtcgc
     4141 aaaggatctg atcaacgggg atgagatcaa ttatccaatc tatgaagagg taaaaaagaa
     4201 gaagtttggt ttattttagt tctttattcg tagataaaaa ggaaatgagg agagagtgag
     4261 agatgtatag aaaaaactca agtggttggt ctaaacatat tgattttatt ttattagact
     4321 tattttgtgt ccagcttgct ttttatatat catatgtaat gcgacagggt gactggaatc
     4381 cgtatgtgat tcctatttat cgccacatgg caatttttat agaacttgag aatcttacga
     4441 ttatattttt atttgaggca tataaaaatg ttttaaaaag aggatattat aaagaggctg
     4501 tagcttctat aaagcaatct tttatgctgt tacttgtgtg cagtttatac cttattacaa
     4561 tgcaggatgg aaatgattat tccagagttt ctttatttat aatgggggtg gtgtatggat
     4621 tattaacata tataattcgt atactttgga aagggttgtt gcatcaccgc atgaaaaacg
     4681 gtgaagatgt atcgttagtt attgtaacat ctcaaaagat tgcaaaaaat gtagttcgta
     4741 atcttaaaga gaagaattat ggcatgtata aacttagtgg tgtgattgta gtagacagag
     4801 atatgtatgg aactaccatc ggaggtgtcc cagtagttgc aaatgctgat aatgcagcgg
     4861 aatatctttt acagaattgg gtagatgaag tattcatcaa tgttgacgaa agtgttccat
     4921 atcccaaaga attaatcgcc agatgttcgg aaatgggact tgctgtacat atgaatctta
     4981 caaaagtgtc tgatagtaca cgtggaaaac aatttattgg ccgagttggt gattatactg
     5041 ttattacatc tagtattaat gttatgacga tgaggcaagc atttataaag agaacgattg
     5101 acattcttgc tggaattatt ggatgtattg caacaggtgt tatttttatc ttcgtagcac
     5161 cagttattta catcaaatca ccagggccga ttttctttgc acaagaacgt attggtcaaa
     5221 atggtaagac atttaagatg tataagttcc gtagtatgta tatggatgca gaagaacgca
     5281 aagcagagtt gatgaaagaa aataagatga gtaataatct catgtttaag atggattttg
     5341 acccaagaat cattggcaat aaaatacttc ctgatggaac aaagaagaca ggaattggac
     5401 aatttattcg atctacatca ttggatgagt ttccacaatt ttataatgtc ctaatgggaa
     5461 aaatgagcct ggtggggttt agaccttgct tgaagtcaga atatgagaag tatgattttc
     5521 atcatcgtgc gagaattgcg atgaaacctg ggattactgg aatgtggcag gtgagtggta
     5581 gaagtgatat cacagacttc gaagaggttg taaaacttga tgcagaatat atcaggaatt
     5641 ggagtatggg attagatttt agaattttgt ttaggacagt caaaactgtg cttcatagaa
     5701 acggttccat gtaagataaa gatactatag aaggagtaaa acgtgatggc aagaaaaaaa
     5761 ttaaaaatct gtatgctagg tcataaaaga attccatcaa gagaaggtgg aattgagata
     5821 gtggtagaag aattggcaac gcgaatggtt gcacttgggc atgaagtgac ttgctataat
     5881 cgaagtggtc atcatgttag cggaaaggaa tttgaccagc ataaaaataa agaatataaa
     5941 ggtgtaaaat taaagactat ttttacatta aatataaaag gaattgcagc aatgagttct
     6001 tcagtatttg gtggaattag agcagcattc ggaaaatatg atattgttca ttttcatgca
     6061 gaaggaccat gtgctatgtt gtggttgcca aaactttttg ggaaaagatg tgttgcaaca
     6121 attcatggtc tagatcatca aagagaaaaa tggaacaagc ttgctagtac atacatcatg
     6181 ttaggggaaa agtgtgctgt aaaatttgca gatgaaataa ttgtacttag tgaaagcgtc
     6241 caaaattatt ttgaagatat atatggaaga aaaacaaggt ttataccaaa tggcgttaaa
     6301 aaaattgaaa taaaatcagc aggattgatt acagaaaagt atggattaac aaaagattct
     6361 tatattttgt ttttggggcg attggttccc gaaaaaggaa ttcggtatct aattgaggca
     6421 tttaaagatg ttcagacaga taaaaaactt ataatagccg gaggaagctc tgatacagat
     6481 gaatttgcaa atgaactaaa ggaattagca aaaggtgatg agcgaattat atttactgga
     6541 tttgtacaag gacaggaatt agaagaatta tatagtaatg cgtatatcta tacacttcca
     6601 agtgatttag aaggaatgcc attaagtttg ttagaagcaa tgagttatgg gaactgttgt
     6661 atagtttcaa atatctcaga gtgtacggaa gtagtagaag acaaggctat gatttttaag
     6721 aaatcggatg taagtgattt aaagataaga ttagaagagg catgcaatca atcggaaatg
     6781 gttaaagttt tgaagaatca agctactgag tttatttgta gtaaatataa ttgggataaa
     6841 atcgtacaag aaacattaaa tttgtatagg ggaaatatat aatgattcga atattacagt
     6901 gtgtgaatga tatgcatcgt gcagggttgg agacgatgct aatgaactat tataggaata
     6961 tagatcgaac taaaattcag tttgattttt tgacacatcg tccatataaa agtgattatg
     7021 atgaggaaat aataagtctt ggaggaaagg tctattatgc accgagactt tatccacaaa
     7081 attatcctaa atattttaaa tggatggaac aattttttaa ggatcatcca gaatatgaga
     7141 ttgttcattc tcatattgat gcgatgagtt atttaccttt aaaagctgca aaaaaagctg
     7201 ggattccgat acggatagca catagccata atacatcgat agatagagat tttaaatatc
     7261 ttttaaagca atattatcga tgtaagttaa cgtctgtttt gacacatgaa ttttcatgtg
     7321 gaaaagtagc aggtgatttt ttgtttcgaa atgataaatt cactttgatt ccaaatgcag
     7381 tggatgctaa aaaattttat tatgatgaaa gtattagaaa tgatgtacga atgaaattag
     7441 gaatttcaag tcagacgttt gtaatgggac atattggaag aatttcatat tctaaaaatc
     7501 atcgtttttt gattgaaatt tttaaagaat ttcacgctat taagaataat tccgtattat
     7561 taattattgg aactggtgat atggaagaag aaataaaaaa ttatgcaaag aaaagtggca
     7621 tagatgatga tattaaattt ttaggaaata gaaatgatat agaaaaattt tatcaagcat
     7681 ttgacgtatt aatgttgcca tcattatttg aaggggttcc gttagtgggt attgaagctc
     7741 aatttgcaga tttaccgtgt ttcttttcgg agaaagttcc tacagaagtt gctttcagtg
     7801 ataagaccaa tttcataagt ttaaatcaat cggcaaaaga atgggcgaga gagattgcaa
     7861 atgtagatat ctctcatcga gattcagaac gttcgatttt aataaaggca gattataata
     7921 ttcaaacagc atacaaaatc ttagaatcaa aatattatga gttatttgaa ttaatacaaa
     7981 gaggttaaag aatatgacac agaaacattc aagtataaaa agaattatac atcgcatatc
     8041 cgaagaattg aatgcaacaa gacgaattgc aaagataaca tcttggaagg aagcatttgc
     8101 cacgttacaa gctaaaattg atattcaagt catgaatcgt aatggatata atgaaagtaa
     8161 aaaacagaag gaacatttat taaaaaaaca tgaggtaatg ctgaagtatt atgaaaagac
     8221 tttttcagat tttttgaaaa catatgatta tagtcaaaat caaataatta atcaatcaaa
     8281 atcaccatat tctaactgca tttgggtttg ttggtggcag ggattggatc aatcgccgga
     8341 aattgtaaaa gcttgtgtaa aatcaataaa acgaaatgca gggaaacata ctgttataat
     8401 tttaacagaa gataattata gacaatatgt tgatatacca aaatgggtgg aagaaaagaa
     8461 aaacaaagga atcattacga gaacaaatta ttctgattta ttaagattat ctttgctagc
     8521 aaaacatgga ggtttgtggt tagacgccac attttattgt acaaaaccaa taccagatga
     8581 gtattttgat atcgatattt ggtctattag gaggcctgat tatgctcacg catccgtagc
     8641 atcaggatat tttgcgggtt attcattagc atgtaatcaa gagtctagat ggatgtttgc
     8701 tataattaga gacttttttt taaattattg gaaaaataat gatatgatgg tggattattt
     8761 gatgatagat tatatgattg ttttagcaca gagaataaat catgaaatag cacagacatt
     8821 tgataaaata cctagtaaca atccattatg tgacgaattg attaaaaatt taaataatat
     8881 atatgatgaa aaagtatgga acttattaaa gaaagatact ttactgttta agttgacatg
     8941 gaaacaatct tttaaaaagc aaaaagatga taaagttact ttttatggga tgttggtaga
     9001 aaacaaatta ttataaatat tagaggtata aaaagttatg aaacacaagg taagtgtgat
     9061 agtaccaatt tacaatgttg aaaaatattt aagaaaatgt gtggatagtt tattgagtca
     9121 gacattaaaa gagatagaaa tcgtattagt tgatgatggt tcgccagatg cttcggggga
     9181 aatagcagat gagtatcaaa aaaaatattc taatgtaaaa acaatacata gagaaaatgg
     9241 ggggttagga ccggcaagaa atacaggaat tgagaatgcg acaggagaat atgtggcttt
     9301 tcttgattca gatgattggg tagaaagtga tatgtatgaa aaattatact tagttgcgtc
     9361 caaagaaaat gctgatatag tagttagtgg acattgtgat tttgcgaatg acaaagctat
     9421 ggtgaaaaag caacatcctt tgagtgggaa tgtatatgat acgcctaata gtattaaaaa
     9481 tgtacgaaaa aatttatttg gacatagtcc aaaagatcaa gaagtagagg ccttcccgat
     9541 gtcagtatgt atgtctttgt ataataaaaa tctgctagat aaatatagac taagatttga
     9601 aaaaatttta tctgaagata caatttttaa cttgaatgca tatgatgtcg caaataaaat
     9661 tgcttttaca gaatatacag attattgtta tagaaaagaa gaacaagagt ctattacaaa
     9721 aagtttatct ccaaataagc agacggaatt taaagaattt ttaataactc taaaagctat
     9781 agcatcgaaa gaagatttag aatgtcaaat cagagcaaaa cgtatggcaa ttgattattg
     9841 taggctttat gtgggcttag ttgatgatgc aaatttggga attaaagaga aaagaaagta
     9901 tataaaaaaa tttattgaag atgagggatt aattcaatgt tggaagggat atcctacaaa
     9961 tacgctacca tttcaacaaa aattttttca taaaatgctt gaatgtaagt gttatacaat
    10021 attgttgttg ttaagtagaa ttagaaagat aattaaaaat atgaccagaa aaaaattgtt
    10081 tataatatta taaactaaaa gtatatcaaa taatagaaat taacaagtta caaaaaaata
    10141 ttataaagaa taatattttc taaaaagtaa actatagtaa tgtggattat caccttccaa
    10201 cagtatataa aatgagcgta aaaacggcta tttttgcctt aacaacgatt tttttgtata
    10261 caaatacgat atgctcatta attattggaa tttgtagtgc tgtttaaatt aatgaaggtg
    10321 taattatatg agtgatcatt ttgaaaaagt ttgttgagaa gatgtgcttc tagtcatgtg
    10381 atgggagaaa ataatttaac tgttagagtg aatagttaaa ttattctggt tatatgaaaa
    10441 aagtagtttc taaaataatc ttgcagggat taacttaaag aaattgagag aatagtttct
    10501 ttgaaatttc tatatacaat gagaagctga aatttacttt tatttgatga ctggaaatag
    10561 aaattaaata tattgtattg ttacattaaa gaaaacttgt atggaaaaat cagtacaata
    10621 ccattattta atgttcttca cttaaagcgc aaaaaatatc agtttattaa actcgtatat
    10681 agaacaaata tatgtagacg gggcgaaacg atgagtgtaa aaaaaatgct atttatggta
    10741 agtacaataa ttgtattaac atcccgattt tggggaatta atactagttt atctatgcga
    10801 tattttatta tggcaatatg ggatttatat tttatgttaa gctatttttc ttatcgtgag
    10861 aaaacatata aaaaatacgt tatcaagaat aacttttggt tgactataat gccgtttgtg
    10921 atattttcta tctatactct aataatatgg ggaattggtt caaatgcaga atttgaaaat
    10981 tttataaagt tatgttccac gttgctttat ttaattcttg catatggtta cgcatgtaca
    11041 gcattttact tatttaaggc agaaggaatt gattttattt tttggtcagg tgttatttca
    11101 tatatgagtg gttcagtttt ttatttaatt gtatcttatg gtttaaatgg tttaatttct
    11161 tatataaaaa gtcttataac aggtgaaaca aatactgcaa attttgctat ggaggtacat
    11221 gatttaactt ttgcgatggg ggtttttttc ctttattacg tattttttga aaataaaaat
    11281 acaaaaaaac ataagataaa aattatcgta tctttaattt taattttcat gggattgaag
    11341 agaattgaac tgttagcttt aagtggtgca attctagtgt attatgtttt attaaagtgg
    11401 ggaaaaaaga ttcaaatccg tgcattagtg tgtactattt gtgcaactgt tatttcgtta
    11461 gtgtttattt acattataga tacaggaatt ttggcattgc ttatgtcaaa tttgggcatt
    11521 acagatgacg gtgcaaggct ttcattttat ctatattcat ctaaatttta tgaacttggt
    11581 ttaggatatg taggaacagg atggacatgg ttttcaaaat acttttttaa tctttattta
    11641 tcgcacttta ggttaaatgg tggaagaatt gcagcaagcc tacacagtga tatattaact
    11701 atttatatag aagttggatg tatagttttt attatatgga tgttttggtt attttttgtg
    11761 aaaccgataa gactaactaa aaaatttgga ttgaaaacag gggagtgtgt attattatta
    11821 actatatata tgtttatatt atatttgacg gataatacgc tcacttatcc agacactcaa
    11881 atgatttttc tcttagtacc tatgatagtt tcgtgggttg ataatcaaca acgaatatcg
    11941 gaatagatag ctaaagtata ttttaattat gaatttataa ttagttgtaa aagttaatat
    12001 ttaacggaca ggaagtaggg tgaaaaaatt tgaaaaaaat tttagtgtta gcaaatacat
    12061 attatcaaat aatagtagca atacaaatga aaaatacaat tttttccgat gagtatgttg
    12121 aaattattat ttctgatcat tctaaaaaca ctaaagaaat tgcgaaaaaa atagaagaaa
    12181 aaaaagtgtt tgatggctgt cattatttag aaagcaaatc ttttgatgat aatattgggt
    12241 attttcaaaa gattaaggac tgtcttcaga tttcttgtgg taaatcaaat cgatatgcct
    12301 attatttgaa aaactgtaaa agtgaatatt ttgatgaatt tatttgtttt tcaatgggag
    12361 aggtttggtt ggaaggaata tatgcttgct tatatcgaaa taataaaaat gtacaggtat
    12421 caattttaga agaaggtatt ttaagctatg gagttgacat tattccaaca gcaagaacaa
    12481 aagctatcgc agtactcaat tacttaaaaa aaaggactcc actatataac aaaagctata
    12541 atttttattg tttttttcct aatatatata caggaagaga gaatacaata caaattccat
    12601 tagttacagc tgggtcaaga accgcattgc aactttcgga tatttttaac cctatgatag
    12661 atatttataa agaaaaaaaa tatatatttt ttacgtctgt ttatgatttt gagggcggag
    12721 aacctgtcgg agaatatgaa ttagtatgca aaatagcaga cttagtagga aaagataatc
    12781 taattgttaa gacgcatcct agagatgtaa gaacaattta taaagataat ggatttatcg
    12841 aggataaaaa ttcatcaata ccttgggagg cgattcaatt gtcaggcgat tttaacgata
    12901 aaatatttat gacaattaat tcaggaagtg ttatttcagg tagtacaatg tctgcaaaac
    12961 ctgtacgcac gtattatatg tataaattgt gtaacataga aggaaatccg tcttgcaaaa
    13021 agaatgcgta tgatattgag cgattgctta agcaaaaaac gatgaaaaac attttaaaga
    13081 atgtgaaaat agcagaaagc ttagatgata tactatgagc aaaactaaat atttaattaa
    13141 aaatatggga atattaacaa taagtaattt ttcttcaaaa attttagttt ttttgttagt
    13201 ccctttatat acaagtgtat tatccacaac agaatatggc acatatgatt tgacaatttc
    13261 aacagccaca ttattgtttc caattttaac cttaaacatt gtagatgctg tgatgcgctt
    13321 tttaatggat aaagataaaa ataaaaaaaa tatagcatct atagcgatta aagttatatt
    13381 tttaagcgtg atagtctttg gatccgtaat gtatttgtta aatattacaa aattatggca
    13441 agatgtttat ggattagaaa aatatatttt tttgtattat atatcctatg tatggaatca
    13501 atttctaata caatttgcta aagggttaga aaaagttaaa gctatgggca tagcaggtat
    13561 tatcagcaca attgtaacaa taattgccaa tattttcttc ttgcttattc tgaaatgggg
    13621 attaattggt tttttcattg caaatatttt atcacagatt atttcagcta cttatctgtc
    13681 agtattagtg agaatatggg aatatatcga ttttgaggct aaagatcctc agctaaagag
    13741 agaaatgcta aattactcga ttccattaat tgcaactgtt gttggatggt ggattaatag
    13801 tacagctgat aggtatgtgg tttcctttat agtaggtgtc tcggcgaatg gcttattgtc
    13861 tgtggcatat aaaatacctc aaataattaa tactttgcag ggaatattta tacaggcctg
    13921 gcaaatttct gcaattaaag aatatggaga taatgagact gcaaaatttt atggaaatac
    13981 atttgttatg attaatttgt taatgtgcat aacctgttca agtcttatca tattaactag
    14041 accgttttca catattcttt atgcgaaaga cttttatgaa gcgtggaaat atgttccatt
    14101 tttattaata gctaatgttt taaattgtgc atcaggtctg ttaggaccta tcttatcagc
    14161 aaaaaaagat tctaaatcca tgatgtggtc tgcaatcata ggggcattag ttaatgtgat
    14221 tatgagtgta gtccttgttt ataagatagg ggttcaaggt gccacaatag ccactgttat
    14281 atgttcatat gtaatatatg ttgttcgtaa acgagctgtg ggaaaagata tttttataaa
    14341 gcattatcat attgttgtgg aatcttgggg attattgttt gttcaggcaa tcatagaaat
    14401 atattttaaa aattatgtaa tagaaattat aattatgatt attttaatta ttataaatat
    14461 cagttatatt aagcagttgt gttatagagg aagaaacatt atttcaatga ttaagagtcg
    14521 agagcacaaa cggtagacga tgttattttt ataaaaagat ataatttatt aatggtcgat
    14581 aattatgaaa ggaaaaatat tcttaaaaaa aagagtatag gatgtcgtat gaaaatagtc
    14641 gctatagtac caatgaagtt aaataacaaa agattgccac aaaaaaacac gaaagctttt
    14701 accaatggaa aaccattgtg ttattatata ctttcaacac ttttaaaagt aaaacagatt
    14761 gataaagtgt atgtctattg tagtaaccca gatattaaag aatttatacc agaaggtgta
    14821 acatttttaa agagatctag ttccttagat caggatacaa cgagtattac acaggtatta
    14881 gccggtttca caaaagaagt agaggctgat gtatatgtaa tgacacacac aacagcacca
    14941 tttattagcg aaaaatctat tgaaaaagga ctagatgccg tgctgacagg agaatatgat
    15001 tctgcatttg ctgcaaaaaa attgcaagat ttcttatgga aagacggtga accatttaat
    15061 tatgaattgg acaatattcc tagaacacag gatttgccgc ctttatacga agaaactagt
    15121 ggattttata tttataatcg caatgtgatg accgagttgc atagaagaat tgggaaaaaa
    15181 ccttgcatag ttgaagtagg tgagattgag tcagttgata ttgatgaagc cgaggatttt
    15241 atgattgctg atgcgattta taatcatctt attttacaag gtagttaaga cagggggaag
    15301 gttaggatga acaatgtaca gattttagat tgcacactta gagatggggg ctattgtaac
    15361 aattgggaat ttggatttga taatacaaaa aaaattattt ctggacttat agaatcagga
    15421 attgagatta tagaatgtgg gtttctcaca aataaaacag tatacaataa aaatgttaca
    15481 aaatatacga ccttagaaga attagcacag ataattcctg aaaaaagaga aggaaaatta
    15541 tttgtagcaa tgatgaacta tggcgaatat aatatagagg atcttccaga ttatgatggt
    15601 tcatctattg atggaattag gattgcgttt cataaaaaaa attgcaatga agcgttgaaa
    15661 ttatgtcgtg agataaagaa aaaaggctac ttagtttttg ttcaagctat ggtatctctc
    15721 aattatacag atgaagagtt tttagcagta ataaaaaatg tgaatcagtt taaaccatac
    15781 gctttttata ttgttgatag ttttggaatg atgaaaggaa aagatttaac tcgtcttttt
    15841 tacatgattg agcataatct agaaaaagat atacagatag gttttcactc acataataac
    15901 atgcagcttg catattctaa tgcacaaaaa cttataaccg tacaaacaaa tcgaagcctg
    15961 attattgatt cttcggtgta cggaatggga agaggagcag gtaatcttaa cacagaacta
    16021 tttgtagaat atttgaatga taatgcagga aaaaaatatc aattaaaacc attattatat
    16081 ataattgatg aaatactaaa tagtttctat cagaagagtt attggggata ttcattacca
    16141 aactatattt cagcagttca taatgctcat cctaattatg caagctatct gtcatataag
    16201 aatactttaa caattgaagc aatgaatgaa atattcgaaa tgatggatga agaaaaaaag
    16261 gtatcttatg ataaacaata tatagaagat ttatactaca aatatatgga aactgggaaa
    16321 atacaggagg aatatatgga agaactgaga aaatttctct cagacaaaaa aattctactg
    16381 attgcaccag gaaaaagttc tgtagatgag gtagaaagaa taaaagagtt tgttaccaaa
    16441 gaagaagtcg taataattag tgtaaatttt gaatattctc atatagatat agattacgta
    16501 tttttaagta atttaagacg ttttcatgaa ttaccgaaag aaagcagaac taaatgtatt
    16561 gttacatcaa atatacaagc atcggatgta tatttacgga cgagttataa aaagttgtta
    16621 tctgaaaggg aagttgtaag agacaatgca ggattaatgg caattagatt tttagccgat
    16681 atgaatgtgg atgaaatatt cttagctgga tttgatggat attcacataa tgaagatgag
    16741 aattatggtg agcaatccat ggaaattata acaaaatttg cattattaga tgcaatgaac
    16801 gtgcaaatga gagatgaatt gtcagaatta gctaaaaaag taaaggtgac atttttaacg
    16861 gatccacgtt gggttcgaat tgataagtag tagtaataat gtttttaaaa ttttgataaa
    16921 gagatagtga gagaataaaa atgggtaaag tcttaaataa aaaagaagaa aaatatttaa
    16981 gggccgatgg ggcgagatga aactctaatt ttgaactttt aagaatcatt tctatattgt
    17041 gtattgttgc ttaccatgct aatttgcgtt taaattataa tgcagacgga attatagtac
    17101 atggttcatt aactgctaac cagattttct tgaattatat tggtatttgg ggtcagctag
    17161 gagttaattg ttttatattt atttcggcat attttttaag tgagaaaaga aacattcact
    17221 ttgaaaatgt tttgggaatt gtattagaat gtatttttta tagtgtatta gtttatggaa
    17281 ttctttgtgc atgtggaata gaaacatttt cattgaacag ctttgtaaaa gaaatattat
    17341 catttgctct aggtagatat tggtttatta catcctattt agcattgtat attctaagtc
    17401 cgtatttaaa tatcatgctt aacaatctgg aaaaacttca gttgcaaaaa ataagtatgt
    17461 tattgtttat aggtgtatgc tgttatcgta tgttatttaa aacatctttg tttggggatt
    17521 gtgctgtttt aattgcacta tatgtcatat gttatacact gaaaaaaacg ataataatat
    17581 ttttgagaga cacagaatca taggaacaat tgtatgtata acagttatac cacttctcat
    17641 atccatagga atacttttca aatttatacc aaatgcaatg gttaaccaga tggttcatat
    17701 tggttctcca ttgatggtta tagcaagtat atttttattt tatgtattta aaaatttttc
    17761 attttacagt aagtatataa ataaacttgc gagaaccagt ttaggggtat atattttaca
    17821 tgaaaatcat tatttcattg atggtatgaa gtctagaggc ttaatatggg atgggatatt
    17881 tggattaggc aaaaggtttt tagataggga tttttggatt tggtttataa ttgcagttgt
    17941 agttgtgtgc ataatttgca tggctatcga tgttgtaaga gaaaaatttg aaagacagat
    18001 ttttaaaatt aagagtgtaa agaacctgtg taaacttatg aatcatgtta taaatatata
    18061 cccataatta ataaatgagg agaaaagaag tgaaagttat atcattcgga gaattattat
    18121 tgagacttgc agcacccggt tattcaaagt tatttcaaag agatagtttg gagactacat
    18181 tctgcggagg ggaagctaat gtagcggtat ccttagcaat atttggatat caatcagaat
    18241 ttataacaaa gcttccggat tcagatgttg gatatgcggc agctaatgct ttgcgttatt
    18301 ttggagtaaa tgttgataat attatatttg gagagggacg catgggactg tattatttag
    18361 aaaaaggagc agctcagaga ccatctaaag taatttatga cagaaaattt tctgcaattt
    18421 cgttagctga tagtgatgaa tttaagtgga aggagatcct aaacgatgca aattggtttc
    18481 attggacagg gataaatcca gcattgtctg agaatctctt tgagacgtcc ttagatgcat
    18541 gtcaggctgc caaaaaaaga ggtataacaa tttcttgtga tttaaattat cgatccaagc
    18601 tttggacatc agaggaagca aaagaaaaga tggaacagat actaccattg gttgatattt
    18661 gtatttgtaa tgaagaagat gcagaaaaag tgctaggaat taaagctgag catacaaata
    18721 ttaatagtgg agaattaaat aaagatggct atgtgcaggt ggctagggag attcacaaga
    18781 agtttggatg taggtatgta gctacaactc tgcgtagaag ttattcggca agtagaaatg
    18841 gttggagtgc aatgttatac gaagcagaaa aagataaagc gtatttttcc acagaataca
    18901 acatacaaat tgttgatcgc gttggtggtg gagacagctt ttctgctggt ataatatatg
    18961 cgttatgtga aaatatgtcc ggacaaaagg ctattgaatt tgcgactgca tctagttgtt
    19021 taaaacatac tatagaaggt gattttaatc gaactacaat tgaggatgta tataatttaa
    19081 tgaatagcgg tggaaatggt cgcgtgttgc gttagtcaac atataaagat aaatcacagt
    19141 aatttttatg gtgattatct gtcttagtag tgattagaat aacgtgaaaa taaattgttg
    19201 agcgtttata ctgttttgaa aaataaattt aacatattga aaaatacaaa tatatacgta
    19261 tattataaag cgccgattgt aaaattaagc tagacaacta aaacagtcat ttgtgctaca
    19321 ctcaaaatag ttccgaccaa agaattataa ggagtgagtg caaataacct atacctatct
    19381 tacaacggat gaacttgtaa taatagagtc ttatttgaaa attaaccaat ctgttgctaa
    19441 aactgcgcat tgcttgaatc gttcaagtca aacgatccat aaagtatatc tgttcttcaa
    19501 gcaaagaaaa tcagccttag aata
//
LOCUS       DASHHB010000052        19072 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.054, whole genome shotgun sequence.
ACCESSION   DASHHB010000052 DASHHB010000000
VERSION     DASHHB010000052.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 19072)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19072)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19072
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.054"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(77..1087)
                     /locus_tag="U2K71_002109"
     CDS             complement(77..1087)
                     /locus_tag="U2K71_002109"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4767 domain-containing protein"
                     /protein_id="HEM7316142.1"
                     /translation="MKAKRLFLLGTILLLGGCSTVSQNEQKKQVDSTTYETETTRKES
                     STKEIETTTQETKTTESSSKATSSDTSDFQIKQELWDTNKASQLATFVPQWGKTLGQE
                     YKSYNPQNNVSLYGTPLPSAVIDGNWKMAINEAPVTVQWSEDGTGNAGFNLVAVYSDV
                     ETGEYLGQHVYFFGFQNGQPKVYLTQQNQGNENNYLYFNETQNQQLKQGFTDIINGQT
                     PQTPVVEESTQQATTTTTQSTSSSGISNWEAAKETVLNNKALWIYESPDKNEANTNII
                     ESTQYRKDLMQDENGEFYPVDISTDDIGNTVMNGGKQFRVYTDGRISQRIGMTNNFQM
                     IK"
     gene            complement(1169..2059)
                     /locus_tag="U2K71_002110"
     CDS             complement(1169..2059)
                     /locus_tag="U2K71_002110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HEM7316143.1"
                     /translation="MANEETVGLRIIEEAFKLPMVKVDRRAFLIQTFQGNVSDIEGLI
                     EKGPQAFLSKEMLDREAQKCIQSITLKSSAVSLATGLPGGIAMAATIPADLLQFFGYS
                     LRLAQEISYIYGFKDLMGETGEFSEEAKNTLILYLGIMLGVTSAGSAVRAMSGKLSTQ
                     ALKKIPQKALTQTLYYPVIKRVLGIFGTKLTKNTFAKGISKAVPVVGGVVSGGINYLS
                     MKPMATKLQTELGKNVAYTEKNLQQDLDILEGEFEEVSSDIVSSDTVLSQLERLSHLL
                     ETNMITEEEFQQLKQELLKK"
     gene            complement(2052..2348)
                     /locus_tag="U2K71_002111"
     CDS             complement(2052..2348)
                     /locus_tag="U2K71_002111"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316144.1"
                     /translation="MKVDGECLTSSYKSIYEFFEEKGLDTDLLYQFYIAFRGQQLSFP
                     MKMYDRELVAQKIETMIQENHSIDSRKLTDRYGFSTRWIQSIIRQQQKEQHVHG"
     gene            complement(2504..3049)
                     /locus_tag="U2K71_002112"
     CDS             complement(2504..3049)
                     /locus_tag="U2K71_002112"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005878891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1643 domain-containing protein"
                     /protein_id="HEM7316145.1"
                     /translation="MIEKELVTTQTEVWRDPHHTHRYVFKRQWAIKGKEEKEKLAVVI
                     TIRPTDTEPFTNDLSMLLIEKNIRQLGFTGFIAVNLFSYTKAKSPRVFSRGNDEQSLE
                     ILTTIFTEKKINTIIFACGSITATSQVAYEQAKTIYDQLSAKQKKMTKVLINAEGKLS
                     HPLSIHVRNEWQLSDHSLLFQ"
     gene            complement(3223..3522)
                     /locus_tag="U2K71_002113"
     CDS             complement(3223..3522)
                     /locus_tag="U2K71_002113"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4'-phosphopantetheinyl transferase"
                     /protein_id="HEM7316146.1"
                     /translation="MLETAIEVLEKCAQLVTTPEEWGYESVTMEKEEIEMGTLPKNVH
                     LPRLVMTHLYIYCAPEDGKDYVVYFITDITSQREFVCGLLVEGRLVWSQIGGTNE"
     gene            complement(3549..3857)
                     /locus_tag="U2K71_002114"
     CDS             complement(3549..3857)
                     /locus_tag="U2K71_002114"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEM7316147.1"
                     /translation="MFQNKGQRYVTKGVMDSLLVELQTLCWNLIDQNVQKKLPLDYLQ
                     IFEFSIEKGNQKLVHRQEEPEARKEYLISPKLRLESVSQKIWVIDSGEYQTMLLPEEY
                     "
     gene            4187..5329
                     /locus_tag="U2K71_002115"
     CDS             4187..5329
                     /locus_tag="U2K71_002115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316148.1"
                     /translation="MKALTISFMEDVFHSLDLEHLNKLAKVLPQKLSGSSIIPKNPSD
                     SFKNRLFNQFLKEKNLRYIFKYISSGYFHDIVANDENIYKSTSIQSIEGFFEEIKNKE
                     LESPFSFYIYLIEQTDEDNIVDFFQNIDGLRTFKNNSLIYIQNKKKNKGCTDISLEYK
                     KMEDLLTLYKKDKIDAENREKVLLKENERLKKKIEKNNNDKIRELSKLEKKFLAEKEC
                     EINRYKEEITNSLLKLKNSESKYLNLEKKFSILNSTLKNKENEIVELKKRCNSLINTS
                     KTVKTISHQTKESIKKILLIGVPGKIVIREDYNFIITNPDTPLEDIIKIIEENDINEL
                     WITTFQLIPRMKKKIIQSLKNKIKIQELNDFTMLMKEENINGFKTR"
     gene            6300..7304
                     /locus_tag="U2K71_002116"
     CDS             6300..7304
                     /locus_tag="U2K71_002116"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM7316149.1"
                     /translation="MLGDSLVVLAGLSGTGKSQLVTSYAKALQLSDTQIKFISVRPFW
                     EDDSDLLGYADTVNSVYRPGDSGLIDALLEASRHKENIYMVCFDEMNLARVEHYFSQF
                     LSVLEMEPNTRKIKLYNEDLENKLYNSSTYPSTIEVGQNILFVGTINTDESTHQFSDK
                     VLDRSNIISLELVPFHEISEVNDYIQSDQKKRNQLKYDDFKNFKNSNPTNGLTQKEKE
                     MFWKLHLAINSKDKNIGIGWRILKQIDEYLKNLPVQNDLSRKEAIDIQLVQRVLSKIR
                     GSDEQLSELVGKWNENDLKQVGEFEQIINEYPESSDFKYTKKILLQKARELNLHGFTL
                     "
     gene            7288..9078
                     /locus_tag="U2K71_002117"
     CDS             7288..9078
                     /locus_tag="U2K71_002117"
                     /inference="COORDINATES: protein motif:HMM:NF016309.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316150.1"
                     /translation="MDLPFSLKLNFGYKSEEQEYELLFFQDEASLLEYTPVKVKEFSN
                     LSFTFNSIDKHAKLYIPELDDYLIENQVKEENGDICYIPSDDNIIIYNRDQGKTVPLI
                     PGYYTLYVKIGETIFYSRFEIILKDLGTPEWEKMKIEIEKMVGGLARTFIPKKTSRRE
                     ITTSNYLMNDQLFQRILEFENNIPQAIVSLESLKKEAKYRIDKRYNWKPIGSKNLVDQ
                     KTISQLQIHPEKRGLLYTPSRIIKYDIIENQWIKFILSYFKNFSLLADRYLEKILQGI
                     IEEKKREDKFTSAYSQSVQNYNTNSYNRKIENLKNNQQKIKHFYAYILEFLDTPVMKN
                     ISSRRPINVPKSLVMNPKYNTIYKMYLKSRNNRNTLNFSNRYQYYWKSTDVLYEIWFF
                     IKIIRSLSLQGYTPISGWIFENDILSQELPFLHEDTTVVLSKNDIRINLVYNNSMEKE
                     YFSNTLDIPVKTSSKRNKPDIRLDIILQDKYYIGSLIFEVKYKKLDNILDNDKGRQRQ
                     QLMAYKQNTMSSILAFPEILTRSLQAVSAVFALYPSNEGRKKTAPKYYEKEGIFFHLL
                     NPSSDEKELSVKIQTSIEERISLFNQQSRN"
     gene            complement(9268..9747)
                     /gene="rlmH"
                     /locus_tag="U2K71_002118"
     CDS             complement(9268..9747)
                     /gene="rlmH"
                     /locus_tag="U2K71_002118"
                     /EC_number="2.1.1.177"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HEM7316151.1"
                     /translation="MNIKIISVGKLKEKYLIQGINEYVKRLNAYAKIELIEVPDEKAP
                     ENLSEAQMRQVKEKEGERILAKIKEQEYVYGLAIEGKNPTSESFAKQIDQLGIQGKSH
                     LVFVIGGSLGLSEAVMKRSNAQISFGKMTYPHQLMRLILVEQIYRAFRINAGAPYHK"
     gene            10175..11464
                     /locus_tag="U2K71_002119"
     CDS             10175..11464
                     /locus_tag="U2K71_002119"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HEM7316152.1"
                     /translation="MDRKNVTPKMKKNKNNSLWRKLGLGLVGGIVGGLLTAGIFYAAM
                     GTGNNSSTATSGNQNSAGETVVENVKVNVDSDITKAVDKVQDAVVSVINLQSQNQSSG
                     FGGLFGQQEESSSSDDSNLEAYSEGSGVIYKKDGNTAYVVTNNHVVDGQQGLEVMMKD
                     GTKVKAELVGTDAYSDLAVLKINSDKVETVASFGDSSALKVGEPAIAIGSPLGSEYAN
                     SVTSGIISSLNRQVTSTNESNETVNINAIQTDAAINPGNSGGPLVNIEGQVIGINSSK
                     IASTSESTSNVSVEGMGFAIPSNDVVNIINQLEKDGKVTRPALGITMVDLSAVSTQQQ
                     EQILKIPSSVTNGVIVTNVQTATPAEKAGLKQYDVITKIDDKEVSSGVELQSALYKKK
                     VGDSVKITYYEGSKEKTTTVELTIDQSALKQNQNSEN"
     gene            complement(11503..12108)
                     /locus_tag="U2K71_002120"
     CDS             complement(11503..12108)
                     /locus_tag="U2K71_002120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4479 domain-containing protein"
                     /protein_id="HEM7316153.1"
                     /translation="MIFAYNKAHVGDTLMVIIADDKGTENTVERKWNVARIKDEQGQV
                     VAWNFFHISDHLTIEGNGQVSLTDEELAKLNRLIKEAGFEEQLEADHEPKIVVGYVKT
                     CVPHPDSDHLSITETEVDNGTVLQIVCGAPNIEAGQKVVVAKPGAMMPDGMMIWPGTL
                     RGVASYGMICSARELHLPNAPEKKGILVLPEDAVVGEVFPK"
     gene            complement(12304..12768)
                     /locus_tag="U2K71_002121"
     CDS             complement(12304..12768)
                     /locus_tag="U2K71_002121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HEM7316154.1"
                     /translation="MNQEYKNILVGIDGSEQALAAFKKALEVARRNNGKVYVANVIDQ
                     QFYNFMGYAPIDQNLVDQQTETAKQMIEECKEYGKSVDYTNIEGIIAYGSVRESMAVK
                     LPKKYAIDLIMVGQSGLNAVERFMTGSVASYVIRHSPCDVLIVSDEEEQPSN"
     gene            complement(12790..13110)
                     /locus_tag="U2K71_002122"
     CDS             complement(12790..13110)
                     /locus_tag="U2K71_002122"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HEM7316155.1"
                     /translation="MIIPNSLEELAGYVETGKSVFFFTAGWCGDCRFIKPQMPEIEND
                     FSDWQFIEVDRDKYIDVAAEWNIFGIPSFVVIQDGKELGRLVNKDRKTKQEIESFLES
                     VIKG"
     gene            complement(13253..14332)
                     /gene="pepA"
                     /locus_tag="U2K71_002123"
     CDS             complement(13253..14332)
                     /gene="pepA"
                     /locus_tag="U2K71_002123"
                     /EC_number="3.4.11.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016632088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamyl aminopeptidase"
                     /protein_id="HEM7316156.1"
                     /translation="MEEKTFQRIKELTELQGTSGFEQDVRAYMESHMEPLVDELQLDG
                     LGGIFGLRHHEEADAPRVMVAAHMDEVGFMLTQIQENGLFKVVPLGGWNPYVVSAQRF
                     TLKTSTGKNYPCISSSVPPHLLRGTSGQKSVEVTDILFDAGFESREEAMSYGVLPGDT
                     IVPYAETIKTANGKNIISKSWDNRYGCTMVLEALEALQNETLGHTLIAGANVQEEVGL
                     RGSKASVNKFKPDLFFAVDCSAADDTVTKNGTFGHLGEGTLMRIQDPGLIMLPRLREY
                     LLDIAETNNIPYQYFVSKGGTDAGAAHTQNEGIPSTVIGVVGRYIHTHQTMFSIRDFE
                     AAREMLIQTLKGLDKSTVNTIVYGK"
     gene            14532..14882
                     /locus_tag="U2K71_002124"
     CDS             14532..14882
                     /locus_tag="U2K71_002124"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HEM7316157.1"
                     /translation="MYEESELSYFKGGLAVGIGLGLLSGVASTIWYHRKKVLSADQVL
                     ENIKTSFLKEGAIEGAWISFEKEPIRKFAVRSSAYRGGLSRIEDGETVYYEFLADAHT
                     GTVLDISRKKGAID"
     gene            15067..16020
                     /locus_tag="U2K71_002125"
     CDS             15067..16020
                     /locus_tag="U2K71_002125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM7316158.1"
                     /translation="MESREKIAVLIPCYNEEATISTVIEDFKRELPEADIYVYDNNST
                     DRTYELAVQGGAIVKKEPRQGKGNVIRQMFFDIDADYYLMVDGDDTYPAEAAHGLLEK
                     LRSGEADMVIGDRLSNGTYFDENKRPFHDFGNNLVKNTITYLYKTKIRDVMTGYRGFN
                     RIFVKSFPIMSSGFQIETELTIHALDKKFKFVEIPIDYRDRPEGSESKLNTFSDGFKV
                     IMMIIKMCKDYKPLLFFGIWTIFFFLFGLVMGIPVIREYMLTSFITRIPSAILSTGLM
                     TLALLSLVTGLILDTVVTNAKKEYELNLYHVYHEYQNKEMK"
     gene            16033..17820
                     /locus_tag="U2K71_002126"
     CDS             16033..17820
                     /locus_tag="U2K71_002126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316159.1"
                     /translation="MHKKKENLFPTFFATLFASGFAFFTSYGLGTLNPTVIIGLAMVL
                     LLAFKQYGIELFSIKNKVAAIARLFFSAAFALTLSIGRQIQFQYVSANYTKTTIADGS
                     IFYFLVYFSIFVYSFLFLQSIYLSMPKLETFFTAQNTQAFSSRKYFLFFTVFLVLCWL
                     PYLLALYPGVVLPDSLSSVAQSMGDVPISNHHPVLFTLLVKLFVHDLPTDTINHGVFL
                     FSIFQTLITASAISYSLCWLLKRNIHFFPVLISLCYFAFAPVFPIYALNMQKDTLFSI
                     ACFLFSLAVFDFIFQREKPTILNQIYVIILALLTTYLRNNGIYVVTGTGLICGLIILK
                     QKRYQAFFQVVLGYTVIAGLLIHPLMARYAIPTETAEQLGVPLQQISRTIVMDGDLSK
                     KDKAFMNQLLPIKDYEAYSPSLADSIKWHKNFNNEFLNTHKKEFLSLWARNLPSNIKI
                     YTDAYILETFGFWVPGTKNSYGFLDTRVNTNNYGMKQVDLIEHLTGLSGMKKIIDARD
                     FFGSGTLLWLAIGSALLAIFKKKYRYLLLFLPGMLVFATIMIATPVAFSLRYVFVLAL
                     GLPVYILSPFLLDKREKMKTDIGDTHETI"
     gene            17807..19009
                     /locus_tag="U2K71_002127"
     CDS             17807..19009
                     /locus_tag="U2K71_002127"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316160.1"
                     /translation="MKLFKKIFFAFVLLIASYCVLLTITFFIPQHSIESNAEKSLQMV
                     EKEGMYPSINQGNMLGTRLDNFTDHLMIRKTKADPELNSLENAMSMADYPRYWHGYQL
                     FLRPLLLGMSLGSIRMIYAAILFLLIGLTSYFLTKCSDIYLTTALLISLAIGNAATFF
                     FSMQFSNIWILTLLSVLLFLTKPQLSNTNQQLFLYFFVIGSLANFFDLLTTPVISWGI
                     PVIIIYYVTNKYLMDKRSSLSKQVGSFVFTGIFWGLGYGLTWVAKWILSSIILNKNIV
                     KDAINQILFRTEGNDKYPLHRLEMLKSNIRLMYPKVAILLLLITCLVFLYFAYRKRNS
                     WRPFQLLVLFVLAAVTPYIWYNILANHSQIHYWFTYRTQIITSFAILSAFAFLIPPEK
                     SKDELNYF"
BASE COUNT         6194 a         3309 c         3128 g         6441 t
ORIGIN      
        1 tataaaaaaa ctgattctaa gaaaattaaa taatttttct caaaatcagt ttttctttga
       61 taaataatat ttttaattat ttaatcattt gaaaattgtt agtcattcca atacgttgag
      121 aaattcttcc atctgtataa acacggaatt gttttcctcc attcattacc gtatttccaa
      181 tatcatcagt agaaatatca actggataaa attctccatt ttcatcttgc attaaatctt
      241 ttctatattg ggtggattca ataatattag tatttgcttc atttttatct ggagactcat
      301 aaatccataa cgctttatta tttagtacag tttctttagc agcttcccaa tttgaaattc
      361 ctgaactact tgtagattgt gtcgttgtag ttgttgcttg ttgagtagat tcttctacta
      421 ctggtgtttg gggagtttgt ccattaatga tatctgtaaa tccttgtttt agctgttgat
      481 tttgagtttc gttaaagtac agataattat tttcattacc ttgattttgt tgcgttaaat
      541 aaactttagg ttgtccattt tgaaaaccga aaaagtaaac atgctgccct aaatattctc
      601 cagtttctac atcagaataa actgctacta aattaaatcc tgcatttcca gttccatctt
      661 ctgaccattg tacagtaact ggtgcttcat tgattgccat tttccaattt ccatcaataa
      721 ctgcacttgg taaaggcgta ccatacagac ttacattgtt ctgaggattg tagcttttat
      781 attcttgacc taatgtcttt ccccattgtg ggacaaaagt tgctaattga gatgctttat
      841 ttgtatccca taattcttgt tttatttgaa aatcactcgt atctgaactc gttgccttac
      901 tagaagactc tgttgtttta gtttcttgag tagttgtttc tatttctttt gtagaagatt
      961 cctttcttgt tgtttcagtt tcataagttg ttgaatccac ttgtttcttt tgttcatttt
     1021 gagagacagt agaacatcct ccaagtaaaa gaatcgtgcc taataggaat aatcgttttg
     1081 ctttcatcct ttttcttctc ctaataataa atttgaattc attcaagaaa aagaaccacc
     1141 cgtataagtg gttgcttttt ctaaataatt atttctttaa cagctcttgc ttcaattgtt
     1201 gaaactcttc ttctgtaatc atattcgttt ccaacaagtg agaaagtctt tctaattgag
     1261 aaagaactgt atcagaagac acgatatcag aagatacttc ttcaaattca ccttctaaaa
     1321 tatctaaatc ttgttggaga tttttttctg tatacgctac atttttccct aactctgttt
     1381 gaagcttcgt tgccattggt ttcatagaca agtaattgat gcctcctgaa acaacgcctc
     1441 ccacaactgg tactgcttta gaaatacctt tcgcaaaggt atttttagtt aactttgtac
     1501 caaaaatacc tagtactcgt ttgattactg gataatacag cgtttgtgtc aatgcttttt
     1561 gaggaatctt tttcaatgct tgtgtagaca gctttccaga catcgctcgt acggcagaac
     1621 ctgcagaagt tactccaagc ataattccca aatacaatat cagcgtattc tttgcttctt
     1681 cactgaactc tccagtctct cccattaaat ccttaaatcc ataaatatac gaaatttcct
     1741 gtgctaatcg taatgaataa ccaaagaact gaaggagatc cgctggaata gttgctgcca
     1801 tagcgatacc tcctggcagt ccagttgcta acgataccgc acttgatttc aaggtaatcg
     1861 attgaataca tttttgtgct tcacgatcta gcatttcttt ggataaaaat gcttgtggac
     1921 ccttttctat gagtccttca atatcagaaa cattcccttg aaatgtttga atcaaaaatg
     1981 ctcgacgatc aactttgacc attggtaatt tgaatgcttc ttctatgatt ctcaaaccta
     2041 ctgtttcttc attagccatg tacatgttgc tccttttgtt gttgtctgat aatactttga
     2101 atccatctcg ttgaaaaacc ataccgatcc gttaatttcc tagaatcaat ggaatggttt
     2161 tcttgtatca ttgtttcaat tttttgagcg actaattctc ggtcatacat tttcattgga
     2221 aacgacaact gttgcccacg aaaagcgata taaaactgat aaagtaaatc cgtgtctagt
     2281 cctttttcct caaaaaattc atagatactt ttatacgaac tcgtcaaaca ttccccatct
     2341 actttcactt gctcacctcc atttcccctg acattttaaa aatatcatat tgattccttc
     2401 attgataatg catataacaa gaggttttac aagttgttta gtctgcaaaa gaacaagtaa
     2461 aataacagaa aaagaaacat atacattgaa tacttatttt cgtttattga aataacaaag
     2521 aatgatccga caattgccat tcattacgaa catgaatact taatgggtga gataacttgc
     2581 cttctgcatt tatcaatact ttggtcatct tcttttgttt cgcactcaat tgatcgtaaa
     2641 tggtctttgc ttgttcataa gccacttgac tcgttgccgt aatcgaacca caagcaaaaa
     2701 taatagtgtt gattttcttt tctgtaaaaa tggtcgttaa tatttccaaa gattgttcat
     2761 cattgccacg agaaaatacc cgaggacttt tcgcttttgt atacgaaaaa agattcacgg
     2821 caataaaacc agtaaatccc aattgtcgga tattcttttc gattaataac atactcaaat
     2881 cattcgtaaa tggttctgta tctgttggac gaatggtaat gactactgct aatttttctt
     2941 tctcttcttt tcctttgatt gcccattgtc gtttgaaaac atagcgatgg gtatgatgag
     3001 gatctcgcca aacttctgtt tgtgtggtca ctaattcttt ttcaatcatc tctttttctc
     3061 ctatgctaat ttttgttcaa gcaattcaat taccattgcc gttataagtg tcaatactag
     3121 ttgaaaatat ttcatattct ttctcctttt ttcttcagaa ttcgataaat cgcggtaagg
     3181 atatcttcta aacatttcgc aatcattctc aaataatcga tcttattcat tcgttcctcc
     3241 aatctgactc caaaccaatc gtccttctac cagtaaccca caaacaaatt ctcgctggct
     3301 ggtaatatct gtaatgaaat aaactacata gtcttttcca tcttctggtg cgcaataaat
     3361 ataaagatgt gtcatcacta aacgtggtag atgaacattt ttaggaagcg tacccatttc
     3421 aatttcctct ttttccatcg taacggattc atagccccac tcttcaggag tagtgactag
     3481 ctgtgcacac ttttccagta cttcgatagc cgtttctaac aagtcatttc gctcctttct
     3541 tttagatatc agtattcttc tggtaataac atcgtttgat actcacccga atcaatgacc
     3601 catatttttt gagacactga ttccaaacga agcttaggtg aaatcagata ctcttttctt
     3661 gcttccggtt cttcttgacg atgaaccaac ttttgatttc ctttttctat cgaaaattca
     3721 aagatttgaa gatagtctaa aggaagcttc ttttgaacgt tttgatcgat taaattccaa
     3781 cataaagttt gtaattccac cagtaaactg tccattactc cctttgtgac ataacgttgt
     3841 cctttatttt gaaacatttc ttttccctcc tttgtcgtaa aaaatagccc aatcttctat
     3901 aaagaagatt ggacttcatg gatataatat atatttgaat ttatctctat ttcattgcta
     3961 tattatttat ttcaattaac tgaatagaca tcacaactaa atataagaaa tgcttttcta
     4021 aaagtatgga gttttcatat ttagtacctc tatactaaat tgcgactaca agtttaaaat
     4081 ttatatagtt attagtaact aagatactta attttgctta atgaactatc ctattaatgt
     4141 tatactacta ttgttattat aaataataca agaaaggaag tcaaaattga aagctttaac
     4201 tataagtttt atggaagatg tttttcattc tcttgatttg gaacatctta ataaattagc
     4261 gaaagtactt ccacagaagt taagtggctc atctattatt cctaaaaacc catctgattc
     4321 ttttaaaaac aggttattta accaatttct taaagaaaaa aatttaagat atatatttaa
     4381 atatattagt agtgggtact tccacgatat cgtcgcaaac gatgaaaata tttataaaag
     4441 tacttctatt cagagcatag aaggattttt tgaggaaatt aaaaataaag agttggaatc
     4501 ccccttttca ttttatatct atttgattga acaaacagat gaagacaaca ttgtagattt
     4561 ttttcaaaat attgatgggt taagaacatt taagaataat tctttgatat atatacaaaa
     4621 taaaaaaaag aataagggtt gcactgatat ctcacttgaa tataaaaaga tggaagattt
     4681 attaaccctt tataaaaaag acaaaattga tgctgaaaat agggaaaaag tattgctaaa
     4741 agaaaatgaa agactcaaga aaaaaataga aaaaaataat aacgataaaa taagagaact
     4801 cagcaaacta gaaaaaaaat tcctcgctga aaaggaatgt gaaataaatc gatataaaga
     4861 agaaataact aatagtttat taaaattaaa aaattcagaa agtaagtatc ttaatttaga
     4921 aaaaaaattc tctattttaa atagcacgct taaaaataaa gaaaatgaaa tcgttgaact
     4981 gaaaaaaaga tgtaatagtc ttataaatac aagcaaaaca gtaaaaacta tttcacacca
     5041 aacaaaagaa agtataaaaa agattttatt aattggtgtt ccaggaaaaa ttgttattag
     5101 agaagactat aattttataa tcactaatcc tgatacacct ctcgaagata tcattaaaat
     5161 aattgaagaa aatgatatta atgaattatg gattacaacc tttcaattaa ttcccagaat
     5221 gaaaaaaaag attatacaaa gtttaaaaaa taaaataaaa attcaagaat taaatgattt
     5281 tactatgctt atgaaggagg aaaatataaa tggttttaaa acaaggtaaa caagattacc
     5341 aaattattgg ttatttggag gatgatttaa ataaaattaa aataaactcg gatgatgtaa
     5401 tgtacttttt tatgactcct ctttcagatt tccccagtag tgtgattgat aatgtagagg
     5461 gtatacaaat atatagtaat acgcttacta aatttgggtt tattgatgat ataaacgaat
     5521 ctcatagtac tcgtgcccaa tcacttaaag aatttttaga tccttatatt atcgtttgta
     5581 caccaaaaat taaaagaaat catgaacaag caagaaatta ctatcaaggt gatgatttag
     5641 aattaatccc caaaccaaag tctgaagttt ttaaaaattt aataaaagag aatgaatcat
     5701 tagtacctat accggtcttt tctataaata acagcgattc tgtaatcaaa gacgagacaa
     5761 gttttgaaaa ccaattacta aacggcaaac tactcggaaa aattataaat ttctcaaaag
     5821 acaatgaaga ttacccttta aatgtttttt gggagaacac tgatggatca agagttttat
     5881 ttggtgaaat aatcggacaa aacaattcaa tgtacggtgt agcatatagg atagaaaaag
     5941 ataattttta caaaaagttt attgatgaag gtagtaaaaa ctatgtaggt tacgatgatt
     6001 ttataaatca aacaaaagat ataatttttg tgccctatct attacttcaa aaaattatag
     6061 aaactaaaaa agatattaac gaaggcatag aaaatatctc tgattccaaa atatctccag
     6121 taagttcaat tattactgaa caaacgcaaa tagatatatc tgagttagaa gataaacacg
     6181 ctattcaaca aaatgatcac ttctctgaac agaatgaatt tatagaaaga tttaatcagc
     6241 tcacaattcg tagtaaatta cattactcaa aacgagactt atggaatttt catactgcta
     6301 tgttgggtga tagtttggtg gtattagctg gactaagtgg taccggtaaa tcacaattag
     6361 taacatccta tgcaaaagca cttcaattat cagatacaca aataaagttt atttctgtga
     6421 gacccttttg ggaggatgat tctgatttat tgggctatgc tgatactgta aattctgtct
     6481 atcgtccagg agatagcggt ctaattgatg cactattaga agcatcaaga cataaagaaa
     6541 atatttatat ggtttgtttt gatgagatga atttagctcg ggttgaacat tatttctccc
     6601 aatttctttc agttttagaa atggagccaa atactaggaa aatcaaactg tataacgaag
     6661 atctagaaaa taaattatat aactcttcaa cctatccttc tacaattgaa gtaggacaaa
     6721 acatattatt tgtaggaaca ataaatacag atgaatctac tcatcaattc tcagataaag
     6781 ttttggatag atcaaacatt atttcacttg agttagtacc attccatgaa attagtgaag
     6841 taaatgacta tattcaatca gatcaaaaga aacgtaatca acttaaatat gatgatttca
     6901 aaaattttaa aaactctaac cctactaatg gtttgaccca gaaagaaaaa gaaatgtttt
     6961 ggaaactcca tttagcaatt aattctaaag acaaaaatat cggtatcggt tggagaatat
     7021 taaaacaaat tgatgaatat ttaaaaaatc ttccagtgca aaatgactta tcaagaaaag
     7081 aagctattga tattcagctt gtacagagag tattaagtaa aatcagaggt tctgatgaac
     7141 aattatcaga attagttggt aaatggaatg agaatgattt aaaacaagtg ggtgaatttg
     7201 aacaaataat taatgaatat cctgagtctt cagattttaa atatactaaa aaaattcttt
     7261 tgcaaaaagc tagggagttg aatttacatg gatttaccct ttagtctgaa attaaacttt
     7321 ggttataaaa gtgaagaaca agaatatgaa cttttatttt ttcaagatga ggcatcgtta
     7381 ttagagtata caccggttaa ggtaaaagaa ttttctaatc taagctttac atttaacagt
     7441 atagacaaac acgcaaaact atatatacca gaattagatg attatttgat tgaaaatcaa
     7501 gtaaaagagg aaaatggaga tatttgctat attccaagtg atgataatat aatcatttat
     7561 aatagggatc aaggtaaaac agtacctctt attccaggtt attacactct ttacgttaaa
     7621 ataggtgaaa ctatctttta ttctcgattt gaaataatac ttaaagattt aggtacacca
     7681 gaatgggaaa aaatgaaaat cgaaattgaa aaaatggttg gcggtcttgc tagaactttt
     7741 attcctaaaa aaacttctcg tcgtgaaatt actacttcaa attatctaat gaacgatcaa
     7801 ctttttcaac gaatattaga gtttgagaac aatatacctc aagcaatagt ttctcttgaa
     7861 agtttaaaaa aagaagcaaa atatagaatt gacaaacgat ataactggaa accaattgga
     7921 agcaaaaatt tagtggatca aaaaactatt tcacagttgc aaatccatcc agaaaaacgt
     7981 gggctactat atacacctag ccgcataata aaatatgata ttattgaaaa tcaatggatc
     8041 aaatttatct tatcttattt taaaaatttt tcacttctag ctgatagata tttagagaaa
     8101 atcctacaag gtattattga agagaaaaaa agagaagata agtttactag cgcgtattct
     8161 caaagtgtac aaaactacaa tacaaatagc tataatagaa agattgaaaa tttgaaaaac
     8221 aatcagcaaa agataaaaca tttttatgct tatatccttg aatttttaga cacaccagta
     8281 atgaaaaaca tttcatcaag aagacctata aatgttccaa aatcacttgt tatgaaccct
     8341 aaatataaca ctatatataa aatgtattta aaaagtagaa acaatcgtaa tactttaaat
     8401 ttttcaaata gatatcagta ttattggaaa agtacagatg tactctatga aatttggttc
     8461 tttataaaaa ttattagatc tctatccctg caaggctaca ctccaatttc tggatggatt
     8521 tttgagaatg atattctttc acaagaattg ccgtttttac atgaagacac taccgtagta
     8581 ttgagtaaaa atgatatacg tattaatctt gtatataata attccatgga aaaagaatat
     8641 ttctccaata cactagatat cccagtaaaa acctcttcaa aacgaaataa acctgatatt
     8701 agattagaca ttatccttca agataaatac tatatcggaa gcttaatatt tgaagtaaaa
     8761 tataaaaaac tagacaatat acttgataat gataaaggta ggcaacgtca acaattaatg
     8821 gcttataaac aaaatactat gtcaagtatt ctagcatttc ctgaaattct tacacgttct
     8881 ttgcaagctg tctctgctgt ctttgcttta tatccttcca atgaaggaag aaaaaaaact
     8941 gcacctaaat attatgaaaa agaaggtatt ttttttcact tattaaatcc ttcatcagat
     9001 gaaaaagagc tttcagtaaa aattcaaaca agtattgaag aaagaatttc attatttaat
     9061 caacaaagta gaaattaaaa agatgaagag cgtctcttta tacaagagag cgctcttcat
     9121 cttcaatttt ttgtaacaaa tttgaataca taataaagtt attattattt ttttgaaatt
     9181 ctcaacgatt ttcaagttaa cttttttata tataaatttc aatgaaaagc taacttagtg
     9241 ttcaggagca ttcaatctta aatatcatta tttatgatac ggagcccccg cgttgatccg
     9301 gaaggcacgg tagatttgct cgacgaggat caggcgcatc aattgatgtg gatacgtcat
     9361 tttgccaaaa gagatctgag cattgcttct tttcatgacg gcttcgctga gtcctaaaga
     9421 accgccgata acaaaaacaa ggtggctttt tccttggata cctaattggt cgatttgttt
     9481 agcaaaagat tcgcttgtgg gatttttccc ttcaattgct aagccataga cgtattcttg
     9541 ttctttgatt ttcgctaaga tccgttctcc ttctttttct tttacttgtc tcatttgcgc
     9601 ttcgcttaaa ttctccgggg ctttttcgtc aggaacttcg atcaattcga ttttcgcata
     9661 ggcattcagc cgtttgacat actcattgat accttggatc aagtattttt cttttaattt
     9721 tcctactgaa ataattttga tattcatgtt ttctctcctt tgatcttcac gctcactttg
     9781 tttattataa tcaatttttt cgtctatatg tttattttga ctatttttgt taaaaagatt
     9841 tccccaacag ttatgcacaa catccacagg tttatccaca gaaaacagcg ttgttttacc
     9901 ttatttcatc acattcatta ggcaaacata cgttttataa gaatttatcc actttataca
     9961 caggctgtgg atatgtttta tcccttattt ttccaatcct atccgcgtag ttttacacaa
    10021 tatctgtgga aaaagaaaat caaatatttt catctgaaaa caatgatttt tcctaaagtt
    10081 tttcataaaa agttcaaagt ctcaaagtag actaaagtca acaaataaga ctatactaac
    10141 agtagataaa tgaatgtaat tggaggagga acacatggat agaaaaaatg tgacaccaaa
    10201 gatgaaaaaa aataaaaata atagcctctg gcgtaagttg ggtctggggc ttgttggagg
    10261 gatcgttgga ggactactga cagctggcat tttttatgca gcgatgggca ctggcaacaa
    10321 tagttctact gctacaagcg gcaatcaaaa ttctgccggt gaaacagttg tggaaaatgt
    10381 caaagtcaat gtagattctg atatcaccaa agcggtagat aaagttcaag acgctgtcgt
    10441 ttctgttatc aacttacaaa gtcaaaacca atcaagcggt tttggcggat tattcgggca
    10501 gcaagaagaa agtagcagct cagatgatag taatttggaa gcttatagtg aaggaagcgg
    10561 cgtaatctac aaaaaagacg ggaatactgc ttatgtagtc acaaacaatc acgtagtaga
    10621 tggacagcaa ggtttggaag tcatgatgaa agacggtacg aaggtaaaag ctgaattagt
    10681 tggaacagat gcctattcag atttagcggt gttgaagatc aattcagaca aagtcgaaac
    10741 ggttgcctct tttggggact caagtgcttt gaaggttggt gaacctgcga ttgcgattgg
    10801 ttccccatta ggatctgaat atgcgaattc tgtcacttca ggaatcattt cttccttgaa
    10861 ccgacaagta acaagtacga atgaatcgaa cgaaacagtc aatatcaatg cgatccaaac
    10921 agatgcagcg atcaacccgg gaaattctgg tggtccttta gtgaatatcg aaggacaagt
    10981 tatcggtatc aactcaagta agatcgcaag tacttctgaa tcgacttcta atgtaagcgt
    11041 agaaggaatg ggatttgcca tcccaagtaa cgatgtagtg aatatcatca accaactgga
    11101 aaaagacgga aaagtgacac gtccagctct aggtatcaca atggtcgact tatctgccgt
    11161 ttctactcag caacaagaac aaatcttgaa gattccttca tctgtaacta acggtgtcat
    11221 cgtgacaaat gtccaaacag ctacaccggc agaaaaagct gggttgaaac aatacgacgt
    11281 cattactaag atcgatgaca aagaagtttc ttctggtgta gaacttcaat ctgctttgta
    11341 taagaaaaaa gtgggagatt ctgtcaaaat cacttactat gaaggctcga aagaaaaaac
    11401 aactactgtt gaattgacga tcgatcaatc ggcattaaaa caaaatcaaa atagtgaaaa
    11461 ttaataaaaa agaggttgtg acaaagactt gtcacaacct ctttattttg gaaatacttc
    11521 tccgactacg gcatcctcag gcaatactaa gattcctttt ttttctggag catttggcaa
    11581 atgcaattca cgagcagaac aaatcattcc atagctggct acccctcgca aagttcctgg
    11641 ccagatcatc attccatctg gcatcatggc tcctggtttt gcaacgacaa ctttttgtcc
    11701 agcttcgata tttggtgcac cgcaaacaat ttgcaatact gtcccattgt ccacttctgt
    11761 ctcagtaatc gataaatggt cagagtcagg atgcgggacg catgttttta catacccgac
    11821 aacgatcttc ggttcatgat cagcttcaag ctgttcttcg aatcctgctt ctttaatcaa
    11881 tcgattcaat ttcgctaatt cttcatctgt taagcttact tggccgttcc cttcaattgt
    11941 tagatgatcg gagatatgga aaaagttcca agcaacaact tgaccttgtt catctttgat
    12001 ccgagcaaca ttccatttac gttcgacagt gttttctgtt cctttatcgt cggctatgat
    12061 aaccatcaaa gtgtctccga cgtgtgcttt gttataagca aaaatcatgt tctttcctcc
    12121 attatccttt gctgagattt atcttaacaa aaaattgcat tcactgcatg agattttttt
    12181 catgctttat ttccgaagaa gcttcttctg gaacaccatt tattcgtaac aagggactgt
    12241 gacaagactt ttgtcacagt ccctttactg tttgattaaa aaggttatac aagttccact
    12301 tgcttaatta ctcggctgtt cttcttcgtc agaaacgatc aagacatcac aaggtgaatg
    12361 acgtatgacg tagctggcta cacttcctgt cataaagcgt tcgactgcat ttagtccaga
    12421 ctgcccaacc atgattagat cgattgcata tttttttggc aatttgactg ccatggattc
    12481 acgaacagag ccgtatgcaa taattccttc tatatttgtg taatcaacag attttccata
    12541 ttctttacat tcttcgatca tttgcttggc tgtttcagtt tgctgatcga ctagattttg
    12601 atcaatagga gcatagccca taaaattata aaattgctga tcgattacgt ttgctacata
    12661 tactttccca ttattacggc gagccacttc taacgctttt ttgaaagcag caagtgcttg
    12721 ttcactaccg tcaataccta ctaaaatgtt cttatattct tgattcatct tgacccctcc
    12781 taacagatgt cagcctttta ttacactttc taggaacgat tcgatttcct gttttgtttt
    12841 acgatcttta ttcactagac gacctaattc ttttccgtct tggatcacga caaaacttgg
    12901 gataccaaaa atgttccatt cagcggctac atctatgtac ttgtcacggt ctacttcaat
    12961 aaattgccaa tcagaaaagt cattttcgat ttcaggcatt tgcggtttga taaaacggca
    13021 gtctccgcac cagccggcag taaagaaaaa tacacttttt ccagtttcta cgtagcctgc
    13081 taattcttct aaactatttg ggataatcat tctttttcct ctactttctg tttatgtatt
    13141 tttcaattaa attatacacc tttctttcga actaacaaac attgatcctc tttttataaa
    13201 aaaaatctga caaaaggttg cttttgccag attttatagt ttcactatcg cgttatttcc
    13261 cgtatacgat cgtgttcact gttgatttat ctaaaccttt caatgtttga atcaacattt
    13321 ctcgtgcagc ttcaaaatcg cgaatactga acatcgtttg atgcgtatgg atataacgtc
    13381 ccactacgcc aatcactgta cttgggattc cttcattttg tgtatgagca gcacctgcat
    13441 ctgtaccgcc ttttgaaaca aagtattgat aagggatgtt gtttgtttca gcgatatcta
    13501 gcaagtactc acgcaaacga ggaagcatga tcaaacctgg atcttggata cgcatcaatg
    13561 taccttctcc taaatgtccg aatgtgccat ttttcgtcac tgtgtcatct gctgcagagc
    13621 agtctactgc aaagaacaga tctggcttga atttattgac agaagcttta gaaccacgca
    13681 agcctacttc ttcttggaca ttcgcgcctg caatcaatgt atgacctaat gtttcatttt
    13741 gtaacgcttc taaagcttct aataccattg tacagccata gcggttgtcc cacgatttgc
    13801 tgatgatatt cttcccgttg gctgttttga tcgtttcagc gtaaggaacg attgtgtctc
    13861 ctggaagaac accgtaactc attgcctctt ctcttgattc aaacccagca tcgaacaaaa
    13921 tatctgttac ttctactgat ttttgtccgc ttgttcctcg taataaatgt gggggaacgg
    13981 aagaagaaat acatggataa ttcttaccag tagaggtttt aagtgtaaaa cgttgagcag
    14041 aaacaacgta agggttccat ccgcctagag gtacgacttt aaaaagcccg ttttcttgga
    14101 tttgtgtcag catgaatccg acttcatcca tatgtgctgc gaccattaca cgaggtgcat
    14161 ccgcttcttc atggtggcgc agcccaaaaa tgccgcccaa accatctaac tgtaactcat
    14221 ctaccagcgg ttccatatga ctttccatat aggcgcgaac gtcttgttca aaaccgcttg
    14281 ttccttgtaa ttctgttaac tctttgattc gttgaaatgt tttttcttcc atatgctatt
    14341 cgtctaaatt tcagactaaa ctcctctcag acttatattg atctgcggat aaaatatgat
    14401 tttttctgca gaccgatcgt atttattata gcttattttt tctggtagcg gttatttatt
    14461 tcaaagaaaa tatgagagtg tggtaaaata aacacaaaga gtagcaagtg agaagaagga
    14521 ggtatatcca catgtatgaa gaaagcgaat taagctattt taaaggcggg ttagccgttg
    14581 ggatcggtct aggattattg agtggggtag cttcaacgat ttggtatcat cgcaaaaaag
    14641 tgttatcagc agaccaagtg cttgaaaata tcaaaacatc ttttttaaaa gaaggcgcaa
    14701 tcgaaggtgc ttggatctct tttgaaaaag aacctattcg gaaattcgcc gttcggtcga
    14761 gtgcttatcg gggtgggctt tcccggatag aagatggcga aactgtttat tatgaatttt
    14821 tagcagatgc tcatacagga accgtattag acatttcaag aaaaaagggc gcaatagatt
    14881 aatacgtcaa aaaaatgaaa taacgaatac gcacccacta tctaaaagag aggtatccta
    14941 tccttctttt atagtgggtg atttttataa aaatagggct atttcctctt ggaatagcta
    15001 gtatttgtag ttattatacg gtattcttga aataagatta gttttattat ataaggagag
    15061 tgacaaatgg aatcacgtga aaaaattgcc gtgctcattc cttgttataa cgaagaggcg
    15121 acaatttcaa ctgttattga agattttaaa agggaattac cagaagcaga tatttatgtc
    15181 tatgataata attcgaccga tcgaacatac gaattggccg ttcaaggcgg agcaatcgta
    15241 aagaaagaac cacgtcaagg aaaagggaat gtaatccgtc aaatgttttt tgatattgat
    15301 gccgattatt atttgatggt agatggtgat gatacttatc ctgcagaagc tgcacatggt
    15361 ttgcttgaga aattacgttc tggcgaagca gatatggtca ttggcgatcg tctatcaaat
    15421 ggaacctatt ttgatgaaaa taagcgtcct ttccatgatt ttgggaataa tctggttaaa
    15481 aatacaatta cttacttata taaaacaaaa attcgcgatg tcatgaccgg ctatcgagga
    15541 ttcaatcgaa tttttgtcaa aagcttccct attatgagtt ctggttttca aatcgaaaca
    15601 gaattgacga ttcatgcttt agataaaaaa ttcaagttcg ttgagattcc aatcgattat
    15661 cgcgatcgtc ctgaaggtag cgaatcaaaa ttgaatacat tctctgacgg attcaaagtg
    15721 atcatgatga tcatcaaaat gtgcaaagat tataaacctt tgcttttctt tgggatctgg
    15781 accatcttct tctttttatt tggattggtc atggggattc ctgtgattcg tgaatatatg
    15841 ctgacaagct ttattacacg catcccttct gcgattttaa gtactggatt gatgacttta
    15901 gcccttcttt cattagtaac agggttgatt ttggatacgg tcgtcacaaa tgccaagaaa
    15961 gaatatgaat tgaatttata ccatgtctac cacgaatatc aaaataaaga aatgaagtag
    16021 gaggcctttt ttatgcataa gaaaaaggag aatctatttc ctactttttt tgcaacttta
    16081 tttgcgtcag gttttgcttt ttttacttct tatggtctag gaactttgaa tccaacagta
    16141 atcattggtt tagccatggt tttgctactt gcttttaagc aatatggaat agagttgttt
    16201 tcaataaaaa acaaagttgc agctattgcc cgattgtttt tttctgcagc ttttgctctt
    16261 acactttcta ttggcagaca gatacaattt cagtatgtct cagcaaacta tacaaagaca
    16321 acgattgctg atggaagtat tttttacttt cttgtttatt tctccatttt tgtgtattct
    16381 tttttatttt tacaatcgat ttatttatca atgccaaaac tggaaacatt ttttactgct
    16441 caaaacacac aggctttctc ttcaagaaaa tattttttat tctttactgt ctttttagtt
    16501 ctgtgttggc ttccttatct gttggcattg tatcctggag tggttttgcc tgattcgtta
    16561 agttcggtcg cccaatctat gggtgatgtt cctataagca atcatcatcc agtcttattc
    16621 accttactgg tcaagctatt tgttcatgat ttaccaactg atacgatcaa tcacggtgta
    16681 tttttatttt cgatttttca aacattgata acagcttcag cgataagtta ttctctttgc
    16741 tggctgttaa aaagaaacat ccattttttt ccggtactca ttagtttatg ctattttgca
    16801 tttgcaccgg tttttccaat ctacgccttg aacatgcaaa aagatacttt gttctctatc
    16861 gcttgctttt tattttcctt agcggttttt gactttattt ttcaacgaga aaagccaacg
    16921 attctcaatc agatttacgt aatcatcctt gctttactga ccacctatct aagaaataat
    16981 gggatttatg tagtaaccgg cacaggtttg atttgcggct tgattatttt gaaacaaaaa
    17041 aggtatcaag cattctttca agtggtgctg ggttatacag tgattgctgg actactcatc
    17101 cacccgttga tggctcgtta tgctattcct acagaaactg ctgaacaatt aggtgttcct
    17161 ttgcagcaaa tttctagaac tattgttatg gatggtgact tgtctaagaa agataaagca
    17221 ttcatgaatc agctactccc gataaaagat tatgaagcgt attctcctag tcttgcagac
    17281 tcaatcaagt ggcataaaaa ttttaacaat gagtttttga atacacataa aaaagaattt
    17341 ctatcactat gggcaagaaa tttaccaagt aatataaaaa tctatacaga tgcttatatt
    17401 ttagaaacgt tcgggttctg ggtacctggt acaaaaaata gttacggctt tttagatact
    17461 cgtgtaaaca cgaataatta tggtatgaag caagtagatt tgattgaaca tcttactgga
    17521 ttgtcaggaa tgaaaaaaat catcgatgct cgtgatttct ttggttccgg cacattactg
    17581 tggttagcta tcggcagtgc acttttagca atctttaaga aaaaatatcg gtatttactc
    17641 ctttttcttc ccggtatgct tgtttttgct actattatga ttgctactcc tgtggctttt
    17701 agtttacggt atgtgtttgt tttagcatta ggtctacctg tttatatcct ctctcctttt
    17761 ttactagata aaagagaaaa aatgaaaaca gatataggag atactcatga aactatttaa
    17821 aaagatattt tttgcttttg ttctcttgat cgctagctat tgcgtactac tgacaattac
    17881 ttttttcatt ccgcagcact ctattgaatc gaatgcagaa aaatctctac agatggttga
    17941 aaaagaagga atgtatcctt ctatcaatca aggaaatatg ctaggcactc ggttagataa
    18001 ttttactgat catttgatga tacgaaaaac aaaagcagat cctgaattga actccttgga
    18061 gaacgcgatg tctatggcgg attatccccg ttattggcat ggttatcagc tttttttacg
    18121 acctttactg ctagggatga gtttggggag tattcgaatg atttacgcag ccattctttt
    18181 tctgctgatt ggattgacga gctacttttt aaccaagtgt agcgacatct atttaacgac
    18241 agccttgttg atttcgcttg ctataggaaa tgctgcaacc tttttcttca gtatgcaatt
    18301 ttcaaatatt tggattttga ctttattgtc tgtattacta tttttaacaa aaccacaact
    18361 ctccaacacc aaccaacaat tattcttgta tttctttgtc attggttctt tagctaactt
    18421 ttttgacttg ttgacaacac cggtcatctc ttgggggata cctgtgatca ttatttatta
    18481 tgtaacaaat aagtatttaa tggataaacg cagttcattg agcaaacaag taggcagttt
    18541 tgtttttacc ggtatctttt ggggattagg ctatggtttg acatgggtgg ctaaatggat
    18601 cctatcatcg atcatattga acaaaaacat tgtaaaagat gcaatcaacc aaatcctctt
    18661 tcgaacggaa ggaaacgaca aatacccgtt gcatcgatta gagatgctga aaagcaatat
    18721 tagattgatg tatccaaaag tagcaatatt attgctattg attacttgtc ttgtttttct
    18781 ctattttgct tatcgtaaac gaaatagctg gcgtccgttc caacttctag ttttgtttgt
    18841 tttagctgct gtaacgccgt atatttggta caatatttta gctaatcact cacagattca
    18901 ttattggttt acgtatcgta cgcaaatcat cacaagtttt gctatactta gtgctttcgc
    18961 ctttttgatc cctcctgaga aatcaaaaga cgaactaaat tacttttaag aataccgatg
    19021 tactaattca tttttaaaca taaaaactcc agctgtcaga gatagccgga gt
//
LOCUS       DASHHB010000053        18688 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.055, whole genome shotgun sequence.
ACCESSION   DASHHB010000053 DASHHB010000000
VERSION     DASHHB010000053.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18688)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18688)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18688
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.055"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..124
                     /locus_tag="U2K71_002128"
     CDS             <1..124
                     /locus_tag="U2K71_002128"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEM7316161.1"
                     /translation="NGLPIYVTAQRNNTSEESVKVESNRIIIQFASSLELVAFK"
     gene            399..635
                     /locus_tag="U2K71_002129"
     CDS             399..635
                     /locus_tag="U2K71_002129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316162.1"
                     /translation="MYWRVYKMKIASEKIDQLTILVNQRIEDIIKEFEGEKIKNIKLT
                     LMDHKFELNSTKRKIVELIEEEIALQINEQQLAN"
     gene            659..1000
                     /locus_tag="U2K71_002130"
     CDS             659..1000
                     /locus_tag="U2K71_002130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316163.1"
                     /translation="MEVEQIKYQGMDRERLIDIIEQQRLELIQKEAVCKEYKKHLEQV
                     IEYHSVEKYRSVVQKNRATSPDSRQDVSISIKTPVFNSVKSEKLESISTALVEKAAEM
                     NSLANEVSRLI"
     gene            complement(993..1184)
                     /locus_tag="U2K71_002131"
     CDS             complement(993..1184)
                     /locus_tag="U2K71_002131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YegP family protein"
                     /protein_id="HEM7316164.1"
                     /translation="MVKNLYFVIREATNGQYYFVIKSNNNEVVATSETYLTKYSAEQT
                     INSIKNGITKDSQVIDMTK"
     gene            1394..2041
                     /locus_tag="U2K71_002132"
     CDS             1394..2041
                     /locus_tag="U2K71_002132"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316165.1"
                     /translation="MVGVVKFLGFTFDGYGTLGEWVGIILIVITIQQTSKYYWYSNSS
                     KIVSRITEGITKKDITVVGKYGFTITLLNEGKSETAIKFCGIIGKYNIFQRFIRKHWY
                     KYIYKYPVVKKLIEKYVDHEISYLNPTDLINQEFQNIGYLEKGLKIPIVNESLVTAMV
                     ANIKNSKKLEKRYKKCKKLKFSFLFLEYNGKEYYQDIYVLRGSNLYKDIKNKISV"
     gene            2301..2645
                     /locus_tag="U2K71_002133"
     CDS             2301..2645
                     /locus_tag="U2K71_002133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEM7316166.1"
                     /translation="MTEEFYRWLLQLIREDRLVKFYQSPKWRRLREKAMKRDHYECQE
                     CRRLGKYHRVENVHHIKEVKDRPDLALDLDNLICLCVEHHNEVHGRYLTALDKQEKKI
                     ESFANFDASERW"
     gene            2650..2931
                     /locus_tag="U2K71_002134"
     CDS             2650..2931
                     /locus_tag="U2K71_002134"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316167.1"
                     /translation="MIINDNGREYDTEKIEEYSSYTQGLIKRLIYVRYVGIRDLLSDN
                     CCSKYKVNQVREALNKDNNVERIKNVFGYSIEEINYYIDFAEAFIPMVR"
     gene            3034..3348
                     /locus_tag="U2K71_002135"
     CDS             3034..3348
                     /locus_tag="U2K71_002135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase"
                     /protein_id="HEM7316168.1"
                     /translation="MFKNELSQNRYREKLRRSLISQLESQKTNIEPFLDNVDRYISLW
                     ETAISLEEDISENGIRLENGKKNESVALLVSVNKQMGLMLDKLAITPELVGEANESIP
                     EL"
     gene            3326..5020
                     /locus_tag="U2K71_002136"
     CDS             3326..5020
                     /locus_tag="U2K71_002136"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase large subunit"
                     /protein_id="HEM7316169.1"
                     /translation="MNQFLSYKHIENWFKAIEEGTIKVCKEQLLLKNYLEERVFTRED
                     IYFDKQMVEDSINIPAQYFPFELIPWEKFLQCFIYGVRWKKDKTLVFNRYLSLMGRGN
                     GKTGFASWNNFFLLTAKHGIKNYDIDIYANNESQAKTSFDDVFKVIKDHPDLDKKVFK
                     ATKEVIQNIATNSKLRYNTANARTKDGKRPGANRFDEIHENEDYSMINVATSGGGKIR
                     DYREFYDTTNGHVRGGPLDDIIEESKMILSGELGIDKDGAEFSSLFPFICRLDNDNEV
                     DDPDMWEKACPTINYNADLKRKMFQEYSQMQRNAGLRLTFMTKRMNRPMEDTRFAVAS
                     YDDVLHTKEKEFPEKMDEVIGTVDFADRRDFASVGLLGKYDKDVYFTQHTFIHESALR
                     LQNIKREVIDISIDQGKSQIVHGKNIEADYIVGWFLEMSNKYYIKKIAMDMYRAKILK
                     PALEEAGFTVEIVRSGSVTHGMLKDLVDDLFINQRLFFGDDAIMRWYCMNVYEEHISN
                     GNIRYEKIEPETRKTDGFFSFLHGLNFLDDIYDSAPVTVTNSSVENTGTGFTPLVF"
     gene            5040..6218
                     /locus_tag="U2K71_002137"
     CDS             5040..6218
                     /locus_tag="U2K71_002137"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage portal protein"
                     /protein_id="HEM7316170.1"
                     /translation="MGIFQKAVGYFTKKATVPLEEYFCKLQVDFVYRKFAIETCIDLI
                     ANAMSKAEFKSYEDGKNKKNDLYYRLNVAPNKKNNATEFRKKLIRRLIFYNEVLIVSP
                     SNNSSEIFIADSWDVTEYALKDDVFSQVQINNIVLDREFLESDVIYIKYADQQIRQLV
                     DAYYQAYGKLISSAMNVYKRSNARRYVLKGNLFRPQDNTTQDQINKMMTSQFKAFMEA
                     DNAGAVFQLQNEYTLEDFSGNFQSNSRDIKNLIDDIFEMTAAAFHVPKNLLKGDMSGL
                     SDQVDAFLMFEIIPIAELIQDAFNASLYEVEEYLSGNFVRVDTTMIKITSFKDLVDAI
                     DVGIRNGVFTINEGRERVGNDRSDKAMADEIFITKNNQQVSKGGEANDDNENISSSKE
                     "
     gene            6181..6867
                     /locus_tag="U2K71_002138"
     CDS             6181..6867
                     /locus_tag="U2K71_002138"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease ClpP"
                     /protein_id="HEM7316171.1"
                     /translation="MTTMKTFLAVKNEGAVPQIFIQGFIGSSWFFEGNTDKGIKNILD
                     SLGDQEEIEVVINSNGGDVFQGIAIGNLLKSNKAKVNVVINGLAASAASIIAMAGDTI
                     KIYNNAQLMIHRASTYGEGNVDDFRTIADQLESIDKSVKASYKTRFNGTDEALQELLE
                     KESFMDAETALSYGLVDEIIDAENSSGTEAKKEQSVEEILNDVKEKRAEKIAAFTAAL
                     NKTFGQGDVK"
     gene            6867..8027
                     /locus_tag="U2K71_002139"
     CDS             6867..8027
                     /locus_tag="U2K71_002139"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage major capsid protein"
                     /protein_id="HEM7316172.1"
                     /translation="MTVKNLKGVTAASDQLMKAFKDGNEESFSAAMVSLSKEIQDKIL
                     EEATAKNQDQLVLMNRGQRVLTTQETKFYNEVVKNEGFAGVEELVPATVFERVFEDLE
                     QSHPLLQKITFVNTTGVTEWIVSRGVNPAWWGKLCEAVKKVLDNGFDVINMKQFKLSG
                     YIPVCKAMLDLGPVWLDRYVRTVLVESLRIALEQAIVDGTGKDMPVGMMRDMSKQTSG
                     EYAEKKAEPITALDAVTMGGLMARLSKFNIEGVNDPIYRNVNPSDVVLIVNPTDYWSK
                     VFPAKTVLTANGEYVQVLPVPVSDLQSTAVPEGKAVIGVASDYFMGVGSTLKIEASDE
                     YHFVEDERIYLAKQYANGQPKRNDSFIVLDISALGTTTTTTKPTTTTTTTQA"
     gene            8037..8912
                     /locus_tag="U2K71_002140"
     CDS             8037..8912
                     /locus_tag="U2K71_002140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316173.1"
                     /translation="MKYILCQPAINRFKWELEVCLTNLKKLGIKDIVLLFSRHDDQIP
                     IFFEKEYGVEVHVYDDLRDDKEYIPSIKPYLWWKYLEEDHSREDDRYFYIDSDVIFNK
                     RINLRKLPSKDDVWYCSDCCSYLSLDYIRSCENGENILKDMANIVNVTVESLETINTN
                     SGGAQWVINRPKANYWKKVYLDSNRLYRYLRGQKTNIKIWTAEMWAQLWNMMYFNIGP
                     KVHEELDFCFATDPIEKVKEVKILHNAGVTTNDEDLFFKGRYVTSTPFDEDLSFVNKK
                     KCSYAYVKAIKAVVR"
     gene            8909..9220
                     /locus_tag="U2K71_002141"
     CDS             8909..9220
                     /locus_tag="U2K71_002141"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316174.1"
                     /translation="MTPEQVTEELLIAVKDNIYVTWNEEDESIKKMIAKNAVYLQSKV
                     STTLSFSPESLEYGLLIERCRYDWNRALDEFEQNFASELLGFIQHYALQEYIAGDVNG
                     E"
     gene            9210..9563
                     /locus_tag="U2K71_002142"
     CDS             9210..9563
                     /locus_tag="U2K71_002142"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage head closure protein"
                     /protein_id="HEM7316175.1"
                     /translation="MANNRRLEETFNDGWLKILTQTTKRNELGKKIGVEDTEITSLKF
                     RNLSMRDSDITAMDAMGSKLTKKVKTPFHPIAKKFNKDQYFIVIDSMRYNVIYADYDN
                     FYIYFYLESVGEYGD"
     gene            9553..9954
                     /locus_tag="U2K71_002143"
     CDS             9553..9954
                     /locus_tag="U2K71_002143"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316176.1"
                     /translation="MVIDNSKEKERLNKQISAIKTSLEEHFGLKLFQDSVGEDELPDD
                     FNYFILETGEIEMITEPKYSVGQNLYLTFYSENREDLTGDSLDIISLIQNRSIRFQRM
                     DPNHLKLENQDRYIDQLVFTFRRLLKSDCHG"
     gene            9947..10351
                     /locus_tag="U2K71_002144"
     CDS             9947..10351
                     /locus_tag="U2K71_002144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316177.1"
                     /translation="MAKNSWELKINGHDELLVRMERYSSESERLINEALKSKGSAIAV
                     DRITEKIPVSEADLRRGHQHAKNSRPLKTQYINLGFIIRPTRKFEYLKYPDLGIGTSK
                     RNQPDEFMRRGLGLALDPITELLIRQFDKLNK"
     gene            10363..10968
                     /locus_tag="U2K71_002145"
     CDS             10363..10968
                     /locus_tag="U2K71_002145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM7316178.1"
                     /translation="MAKTTTVTTFDNISIKRISFNFKNATNAISTDCNGQLDGETEMQ
                     RIVKKCGSTEVKSKSKPINMTVTITAHVPMEVYRRFNGLKQDERIKSGIYSYGPDSVG
                     EDFSLAAEIVDDFEEKNKLIGMLACTSNTGLTFSIENGADEVAALELETKVMQDEFGK
                     FYHEAIVAELEEDLTDQWMTNLSADVIKKTSVVTTTATQSQ"
     gene            10988..11350
                     /locus_tag="U2K71_002146"
     CDS             10988..11350
                     /locus_tag="U2K71_002146"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316179.1"
                     /translation="MNEDYSKIELNDGTILNLEPKLNIKKLLMINRDFNTDEFAKMTV
                     GKGSMDISVIQGAKAVYIAYRQANMTDYISFDEFIDKWDFDMATASYIYQLMMFKQAR
                     DAYQKEFEKANKEKKLQK"
     gene            11353..11535
                     /locus_tag="U2K71_002147"
     CDS             11353..11535
                     /locus_tag="U2K71_002147"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316180.1"
                     /translation="MPKLLVETWVDVYSMLTDVFSMPSDLVLSDICLDDILQMAYNKS
                     AYEGWKNYAINQSQKN"
     gene            11552..14971
                     /locus_tag="U2K71_002148"
     CDS             11552..14971
                     /locus_tag="U2K71_002148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail tape measure protein"
                     /protein_id="HEM7316181.1"
                     /translation="MAKKRTEAEVTFIANDDGLKSTLKEISAELTKNRAELKLEQAQL
                     QQTGSESDKLGSKLSSLEKQYELQSQKVEVTSQRLANAKKYYGENSTEVQKLERELIN
                     QQTAQQRLSNEIDKTSNALAQAKGEIQTYESTMQQLDSEQKNVQASASLIESEYKKWQ
                     ATAGQSASESEKLAKAQEYVSQQSENAEKTIDILRRQLEATQSEFGATSTEAMQMEAK
                     LNDAEREFEELGQAAKNVDTTNLDDIGSKIDMNNLMEASDVLSDIGDKLTELGKQAVD
                     SANSVGSSQSKIQANFGLTKQEAEELTNVARDIYYKGFGESLDQSTDALILVKRNLGD
                     LNNQDLQNITEQAMVLENTMGADMDETLRGVNGLMVNFGLSAQDAMDLMVSGTQNGLD
                     KTHELGDNMAEYSQLWSQMGYSADETFGMLQNGLDAGAYNLDKVNDLVKEMGISLTDG
                     RFEQNMDMFSESTRKAFEEWKNGGGTQKDVINSMIQDFSNMDGQYDQLNKASTIWSAL
                     GEDNAMKVVQSLTDVNHTFDDVSGSAQKMNEDSTTPLQELNGKIAELKDSLAPIGNTI
                     IDALEPVIDFLGKMADAFNNLPQPVQDYAVAIGGLTAAFTLLMPIIVGFMALGGPTTL
                     IIGAVITVIAGVIAIIKNWGAITDWFKGIWSKFTDWLGGTWESIKEGASSVWDGVKET
                     WSGFVDWVQDIWQGVSDWFGELWSGLVEGASNIWQGVQETWQAFVDWVSNIWNGVKEV
                     WSIIWADIVGIVQIPWTLITSLIQAGINIIVGIFDVAGQLLGAAWQAVWTPISDFLKN
                     TWDTMTQWVSIAWNGIVTTFHTIFDPVVAWWNGIWTAISTTASNIWNSISATASSIWN
                     SIKNTITSLVQAAATVIQNIWSTVSSWLGGIWNSISSTASNIWNSVTSSISNAINAAK
                     SAIQSVWNSISSWISGIWNGIKNTALNLWNGITSTISSKVNDGKNAISSGWSNLTGIV
                     SDIFNNVKSTIANIWEGIKKTVSAPIDWIRDKISSIFDNLNISIPHIPLPHFKLSGEF
                     NPLKGKIPTLGVDWYAKGSVFNSPNIIGVGEAGPEAVLPLKRSVLQEIGDRILSSTSV
                     SSRAQTIQPVNNYEFNFTIDGNADEVTMKQTTQQIIDSITKVQNDNASAWR"
     gene            15022..15759
                     /locus_tag="U2K71_002149"
     CDS             15022..15759
                     /locus_tag="U2K71_002149"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM7316182.1"
                     /translation="MTDCIHSIIDGFPDYLHKLALAERPTIPSPKRQRVETSVLGRLG
                     GLVQDYSFEDMSFTLHYNYLEDVEDHQAFKQSFYIMRHWLNYAKKLEFSDDPNVYYVI
                     QTIDIGDAENDIVEWGEFDVNITAKPFARVQEDVPITVDKPQSFNLLNNSLEESFPKI
                     IITPSATSCQFILNDYVFSFEGLVVGTDVVIDSDLMLCYEEQSDGDILDRSNKMKTMQ
                     YPTLQVDINYFNCTGLSKIQIYRNGLR"
     gene            15769..18060
                     /locus_tag="U2K71_002150"
     CDS             15769..18060
                     /locus_tag="U2K71_002150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage tail protein"
                     /protein_id="HEM7316183.1"
                     /translation="MIDNLITIYDKNDANNLAEHLYDTQGLGALSDWLTATVSNKLNG
                     AEIFQGTYPISGTNADLIVEGRIIQCYVDENRAKQRLRIYYAKTSVIGNTIEVKAEPI
                     FNDIRKSVLNKYDSGTEKITASQAWQNAKTLAKPVIPSQFSFSSLVDTLANVKIEKAN
                     FLEFFGGKEGSILDRFHGEFLKDNNTLRHEKRLGTDHKIKAIYTKNLTGLDLEIDAQS
                     VLVGVYPFISSSSEGEDEITLPEEVIFTDYVDDYPAGYVSFVDFKDKATDVATLREAA
                     KDWLKTNIDKQKPQVSGSIELVPLRHQRGYEKFVDLEKVSMGDGVDVYHPQLKVNMSA
                     RIVEYTFNVLTNSYDKLVVGNVKTNFLENTENNVSNLINDAIDQLKNGGEISDLLNDI
                     VDHQTDMITGQNGGYVLLDPKEAPSRILIMDTPDKNTARNVLQINNAGIGFSKTGING
                     TYETAWTLDGGFNASFITAGEIVGITIRGTTLISDGADYRTSIANGKMTWYSKKVNKD
                     IMELEARDYVSADAGIVSYTMKTGGGFMIRNPQGNLVFSTWDNGNNRPFLSFGAPNFR
                     YSNASYVTSGDGSSLSINGSAGNSWEFKVAGRTMKFTSDGMLTLPGCFFGSWEDGKLA
                     RFEQSTVQVYKDFTVRGTKNSTVPTEHYGQRLLNAYETPEYYFADYGEAVTGDNGKVR
                     VDIDPMFAETVNLSRYMTHVTPTELVLCAVTHEDIDHFIIETSKPNVLVRWNLVAHRL
                     GYEDIRLKEDTAYDSTVLDQKRF"
     gene            18084..>18688
                     /locus_tag="U2K71_002151"
     CDS             18084..>18688
                     /locus_tag="U2K71_002151"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316184.1"
                     /translation="MASSLYNLALDFSKELNYTKAIMARQGDKGITVTVKPFLNGLQM
                     DTSGGTFTLKGTTPSNRYVDNVATSVTSEEVTFSLDGTFMSEAGYYKHCYVEYRKDNQ
                     ILTTQDIIFFSLGVSDISQGQADEYVSQLEELIRKYNETFDAFMAEIKGRVDSLNQQI
                     TDLTGQAKTLQDKLDALKEEISKLGNLQVMYSNSIDFGNYD"
BASE COUNT         6659 a         2703 c         3871 g         5455 t
ORIGIN      
        1 caatggtctg cccatttatg taactgctca aaggaataat actagcgaag aatcggttaa
       61 agtagaatca aacagaatta tcatccaatt tgcttcatcg ttagaactgg ttgctttcaa
      121 gtagccagtt tttacacttt ttataccctt ttattaccaa tttggtttcc attttatacc
      181 ttttttatac caatcactta cctattcaac gttgtattat gatagtgtcg aaagattagg
      241 aaacaggatc gacaaaataa aatgtaaggg aggaaatctc cctcatcgtt taattaagct
      301 tcgataggca gcagcggaaa tattaagaat aaggatgtga atttcaactc cttctaaatt
      361 gttcttatta tctatcatcc gttgctgtct attaatttat gtattggagg gtatataaaa
      421 tgaagatcgc ttccgaaaaa attgatcaac taacaatttt agtaaaccaa agaatcgaag
      481 atattatcaa agaattcgaa ggtgaaaaga ttaagaacat aaagcttact ttaatggatc
      541 ataagttcga attaaattcg acaaaaagga agatcgttga gcttatagaa gaagaaatag
      601 ctctccagat caatgagcaa caattagcta attaatttat gtattggagg ggaaatgaat
      661 ggaagtcgaa caaataaagt atcaaggtat ggatcgggaa cggttgattg atattattga
      721 gcaacaacgt ttagaattga tccaaaaaga agctgtatgt aaagagtaca aaaaacattt
      781 ggaacaagta attgagtatc attcagtaga aaagtacaga tcagtggtgc aaaaaaatag
      841 agcaacgagt cctgattctc gtcaagatgt atcgattagt atcaagacgc cagtatttaa
      901 ttctgttaaa tcagaaaagc tagaaagtat ctcaactgct ttggtagaaa aagcagctga
      961 aatgaactcc ctagcaaatg aagttagtcg tcttatttag tcatatcgat aacttgagag
     1021 tctttggtga ttccgttttt tatagaatta atggtttgtt cagcggaata tttagtcaag
     1081 tatgtttcgc tagttgctac tacttcattg ttattggact taataacgaa gtaatattgg
     1141 ccattagttg cttctcttat aacaaagtac aaatttttca ccacctataa tttatttcag
     1201 cggaccactc gctgataatt aaaattataa gctatgtata aaatttcaca atatcaattt
     1261 gtcgctgtgg cggaaagggt agacgcaata aaggatgaat gggaaaagtc aatgagtaat
     1321 tatctgaaat ttacaagtcc cctgtttagt tatctaagat tatttgataa tattatttct
     1381 gaggtgatag aaaatggtgg gcgtagttaa atttcttggg tttacatttg atggatatgg
     1441 tacgttaggc gaatgggtag gtattatttt gatagtgatt acaattcaac aaacatcaaa
     1501 gtactactgg tattctaata gttctaaaat tgtatcaaga ataactgaag gaataaccaa
     1561 aaaagatatt actgtcgtcg gtaaatatgg gtttactata actttattaa atgaagggaa
     1621 atctgaaacc gcaataaagt tttgcgggat tataggtaaa tataacattt tccaacgttt
     1681 tatcagaaag cattggtaca aatatattta caaataccct gtagttaaaa agcttattga
     1741 aaaatatgtg gaccacgaaa tatcttatct caatccaaca gaccttatta atcaagaatt
     1801 ccagaatatt ggatacttag aaaaaggtct aaaaatacca attgtaaatg aaagtttagt
     1861 tactgcaatg gtagctaaca taaagaacag caagaaatta gaaaagagat ataaaaaatg
     1921 taaaaaattg aaattttcct ttttattctt agaatataat ggcaaagaat attatcaaga
     1981 tatttatgtt ttgcgaggct cgaacttata taaagacatt aaaaataaga tttcagtatg
     2041 agatttttta aaattcgtag atcactcttt gagtggtctt tttattttgc gtaaaggagg
     2101 cacgacgatg tatagaccac aatacttaga acagaagcat gaagtaatca ctgtgcaaaa
     2161 cggtaacggt gagatagtac gaaagtatag aagaccaata aagagcgata catataaacg
     2221 gaaggaaagc aatgaagtta ttccattgta tggcaaaaga atagctaagt attaaataag
     2281 attgcgaaag gagatgggac atgaccgagg aattctatag atggttatta cagttgataa
     2341 gagaagatcg tttggttaag ttctatcagt ctcctaaatg gcgcaggctt agagagaaag
     2401 cgatgaaacg agatcactat gaatgccaag agtgtagaag actaggtaag tatcatagag
     2461 tagagaacgt tcatcatata aaggaagtca aggatagacc tgacttagct ttagatttag
     2521 ataatcttat ttgtttatgt gttgaacatc ataatgaagt tcatggcaga tatcttacag
     2581 cgttagataa acaagagaag aagatagaaa gcttcgctaa cttcgatgca agtgaaaggt
     2641 ggtaagtgca tgatcatcaa tgacaatggc agagagtatg atacagaaaa gattgaagag
     2701 tattcatctt atactcaggg attaattaaa cgtttgatat acgttcgcta tgtaggtatt
     2761 agggatctgt tatcagataa ctgttgtagt aaatacaaag tgaatcaagt aagagaagcg
     2821 ttgaataaag ataataacgt cgaaagaata aaaaatgttt ttggatatag tattgaagag
     2881 attaattatt acattgactt cgctgaagct ttcattccga tggtgagata accccccctt
     2941 aaaataaatc gcaaattttt tgggggtgat gaaacggagg gggctgtcag gaaaagagat
     3001 tttttcgaac tttatcatga aaggagggct aaaatgttta aaaacgaatt gtctcaaaat
     3061 cgctacagag aaaaattacg ccgctcttta ataagccaat tggaaagtca gaaaacaaat
     3121 attgagccat tcttagataa tgttgatcgt tatatcagtt tatgggaaac ggcgatatca
     3181 ctggaagaag atatatccga gaacggcatt agactggaga atggtaaaaa gaatgaatca
     3241 gtagcgttgc ttgtttctgt caacaaacaa atgggattga tgttggataa acttgccatt
     3301 actcctgaat tggtaggtga agcaaatgaa tcaattcctg agttataagc atattgaaaa
     3361 ttggttcaaa gctatagaag aaggcactat caaggtatgc aaagagcaat tattgctaaa
     3421 aaattatcta gaagaaagag tctttactag agaagatatt tacttcgata agcagatggt
     3481 agaggattca atcaatatac cagcacaata ctttccattc gaattaattc cgtgggaaaa
     3541 atttctacaa tgttttattt atggtgttcg atggaaaaaa gataaaacac tagtgttcaa
     3601 tagatatctt tcattaatgg gacgtggtaa tggtaaaact ggttttgctt cttggaacaa
     3661 cttctttcta ctaaccgcta aacacggtat taaaaattat gatattgata tctatgccaa
     3721 taatgaaagc caagcaaaga ctagttttga tgatgtattt aaagtaatta aagatcatcc
     3781 tgatttagat aaaaaagtat ttaaagctac gaaggaagtt attcaaaata tcgctacaaa
     3841 cagcaaactt cgttataaca cggcaaatgc tagaacaaaa gatgggaagc gaccaggtgc
     3901 aaaccgcttt gatgaaattc acgaaaatga agattattca atgataaatg tggctacttc
     3961 tggtggtggt aaaattcgag attatagaga attttatgat acaactaatg gtcatgttcg
     4021 tggtggtccg cttgatgaca ttatagaaga atcaaaaatg attctttctg gagaacttgg
     4081 aattgacaag gatggagcag aattttctag tttgtttcca tttatttgtc gcttggataa
     4141 cgataatgaa gttgatgatc ccgacatgtg ggaaaaagct tgtccaacta ttaattacaa
     4201 tgcagattta aaacggaaaa tgtttcaaga atactctcaa atgcaacgta atgctggttt
     4261 aagacttacg ttcatgacca aacgaatgaa cagacctatg gaagatacac gatttgctgt
     4321 tgcttcatat gatgatgttc tgcatacgaa agaaaaagaa tttcctgaaa aaatggatga
     4381 agtgatagga acagtcgatt ttgctgatag acgagatttt gccagcgttg ggttgctagg
     4441 aaaatacgat aaagatgttt attttacaca acatactttt atccacgaat cagcccttcg
     4501 attacaaaac atcaaacgag aggttataga tatttctata gatcaaggaa aatcacagat
     4561 cgttcatgga aaaaatatag aagctgatta tattgtaggt tggtttcttg aaatgagtaa
     4621 taaatattat attaaaaaaa tcgctatgga tatgtaccgt gcaaaaatat tgaagcccgc
     4681 tttagaagaa gcaggtttta ctgtggaaat tgttcgaagc ggatctgtta cacatggtat
     4741 gttaaaagat ctggttgatg acctttttat taatcaacgt ttattttttg gtgacgatgc
     4801 gattatgcgt tggtattgca tgaatgtata tgaagagcat atttctaatg gaaatatacg
     4861 ctatgaaaaa atagaacctg aaactagaaa aacggatggc tttttttcat tccttcatgg
     4921 tttgaatttt ttagatgata tttatgattc tgctcctgta acagtcacaa atagctcagt
     4981 agaaaataca ggaactggat ttactcctct agtattctaa cttgaaagga ggtgagaaag
     5041 tggggatttt tcaaaaggcg gtaggatact tcacaaaaaa agcaacggtt cctttagaag
     5101 aatacttttg taaattgcaa gttgattttg tgtatcgaaa atttgcgatt gaaacttgta
     5161 ttgatttgat tgcaaatgcg atgagtaaag cggaattcaa gtcatatgaa gatggaaaaa
     5221 ataaaaagaa tgatctttac tataggctga atgtagctcc taataagaaa aataatgcaa
     5281 cagaatttag aaaaaaactg atcaggagat taatattcta caatgaagta ttgatcgttt
     5341 ctccgtctaa taattctagc gaaatattta ttgcggatag ttgggatgtc acagaatatg
     5401 cattgaaaga tgatgtgttt tctcaagtgc aaattaacaa catagtcctt gatagagaat
     5461 ttctagaaag tgatgttatc tatataaaat acgcagatca acaaattagg caactagtcg
     5521 atgcgtatta tcaagcgtat gggaaactca tttctagtgc catgaatgtt tacaagcgct
     5581 ctaacgctcg tagatacgta ctgaaaggga atttattccg accgcaagac aatacaacac
     5641 aagatcaaat caataaaatg atgacatcac aatttaaggc ttttatggaa gctgataatg
     5701 caggtgcggt atttcaatta caaaatgagt acacattaga agatttcagc ggaaacttcc
     5761 aaagcaattc aagagatata aaaaacttaa tagacgacat ctttgagatg acagcagcag
     5821 cgtttcacgt tccgaaaaac ctactaaagg gagacatgag tgggttatcg gatcaagtgg
     5881 acgctttttt aatgttcgaa atcataccga ttgctgaact tattcaggat gcgtttaacg
     5941 ctagtctcta tgaagtagaa gaatacttgt cagggaattt tgtacgtgtg gatacaacta
     6001 tgatcaagat tactagcttc aaagatttgg ttgacgctat tgatgtaggc attagaaatg
     6061 gagtatttac aatcaacgaa ggaagagagc gcgttggaaa tgatcgctct gataaggcga
     6121 tggcagatga aatatttata actaaaaaca atcaacaagt atcgaaagga ggtgaggcga
     6181 atgacgacaa tgaaaacatt tctagcagta aagaatgaag gcgcagtacc gcaaattttt
     6241 attcagggat ttattggttc tagttggttc tttgaaggga atactgacaa gggaatcaaa
     6301 aatattttgg atagtctagg tgatcaagaa gaaattgaag tagtaattaa ttcaaacggt
     6361 ggagacgtat ttcaagggat tgctattggg aacttactta agtcaaataa agcaaaagtt
     6421 aacgttgtga ttaacggctt agccgctagt gctgcttcaa ttatcgcaat ggctggcgat
     6481 actataaaaa tctacaacaa tgcacaattg atgattcacc gcgcttccac atacggagaa
     6541 ggaaatgtcg atgacttccg tacgattgct gaccaactgg aatcaattga taaatcggta
     6601 aaggcttcat ataaaacacg attcaatggc acagatgaag cattgcaaga acttcttgaa
     6661 aaagaatcgt ttatggatgc agaaacagct ttgagttatg gattggtcga tgaaattatc
     6721 gatgcagaaa atagctcagg tactgaagct aaaaaagaac aaagcgttga agaaattttg
     6781 aatgacgtta aagaaaaaag agcagaaaaa attgctgcat ttacagcagc attaaataaa
     6841 acatttggac aaggagatgt aaaataatga cagttaaaaa tttaaaaggt gtaacagctg
     6901 caagcgacca attgatgaaa gcttttaaag atggtaacga agaatctttt agcgcagcta
     6961 tggtaagctt atctaaggaa attcaggata aaattttaga agaagcaaca gcaaaaaatc
     7021 aagatcaatt agtattaatg aaccgtggtc agcgtgtatt aactacacaa gaaacaaaat
     7081 tctataacga agtagtgaaa aacgaaggtt ttgcaggggt cgaagaatta gtgccagcta
     7141 ctgtatttga acgtgtattt gaagatttag aacaatctca tccactattg caaaaaatta
     7201 cttttgttaa cacaactggt gtaacagaat ggattgtgtc acgtggagtc aatccagcat
     7261 ggtggggtaa actttgcgaa gctgttaaaa aagttttaga taatggcttt gatgtaatta
     7321 acatgaagca gttcaagcta tcaggttata ttcctgtatg taaggcaatg cttgatttag
     7381 gtccagtatg gttagatcgt tatgtccgta ctgttttagt agaatcattg agaattgcat
     7441 tagaacaagc aattgttgat ggtactggta aagatatgcc agtcggaatg atgcgtgaca
     7501 tgagcaaaca aactagcgga gaatatgctg aaaaaaaagc agaacctatt acagctttag
     7561 atgctgtaac tatgggcggt ttgatggcgc gactatcaaa attcaatatc gaaggtgtga
     7621 atgatccgat ttatcgtaat gtgaatcctt ctgatgtggt cctaattgtg aatccaactg
     7681 attactggtc taaagtattc ccagctaaga ctgtactaac tgctaatgga gaatacgtac
     7741 aagtattgcc agtaccagtt tcagatttgc agtcaacggc tgtgccagaa ggaaaagcag
     7801 ttattggggt agcctcagat tacttcatgg gtgtaggatc tacactaaaa attgaagctt
     7861 cagatgaata ccattttgtt gaagacgaac gcatttatct agctaaacaa tatgcaaacg
     7921 gtcaacctaa acgtaacgat agtttcattg tattagatat tagcgctttg ggaactacta
     7981 ctacaactac aaaaccaaca accacaacaa ctacaacaca agcgtaggtg atcagaatga
     8041 agtatattct ttgtcagccg gcaatcaatc ggtttaaatg ggagcttgaa gtttgtttaa
     8101 ctaatctgaa gaaactagga atcaaagata tcgtattgct tttcagcaga cacgatgatc
     8161 agattcctat tttttttgag aaggaatatg gtgttgaagt tcatgtgtac gatgatctgc
     8221 gggacgacaa agagtatatt ccttcgatta aaccatattt atggtggaaa tatttagaag
     8281 aagatcattc gcgtgaggac gaccgatatt tctatatcga ttcggatgtc attttcaata
     8341 aaagaattaa tttgcgcaaa ttgccttcta aagatgatgt ttggtattgt agcgactgct
     8401 gtagttatct aagtcttgat tatattagaa gctgtgaaaa cggagaaaat attctaaaag
     8461 atatggcaaa cattgtaaat gttacagtag aatctttgga aactataaac actaattcag
     8521 gaggcgcaca gtgggttatt aaccgcccta aagcgaatta ttggaaaaag gtttatctgg
     8581 attctaatcg gctatatcgc taccttagag ggcaaaaaac aaatataaaa atctggacag
     8641 ccgagatgtg ggcacagctt tggaacatga tgtatttcaa tattggtcct aaagttcacg
     8701 aggaattaga cttttgtttt gctactgatc caatagaaaa agttaaagaa gtaaaaatct
     8761 tacacaatgc tggagtaaca acaaacgatg aagatttatt tttcaaaggg agatacgtga
     8821 cttctacgcc ttttgatgaa gatttatcat ttgtaaacaa gaaaaaatgc tcttacgcat
     8881 atgttaaagc aattaaggcg gtggttagat gacgcctgaa caagtgactg aagaattgct
     8941 aatagctgtg aaggataata tttacgttac ctggaacgaa gaagatgagt caattaaaaa
     9001 gatgatagct aaaaatgctg tttatcttca aagtaaagtg agtacaacac tttctttttc
     9061 tcctgaaagc ttagaatacg gattgctaat cgaaagatgt agatacgact ggaatcgtgc
     9121 tttagatgag tttgaacaaa atttcgctag tgagttatta ggtttcattc aacattatgc
     9181 gctacaagaa tatattgcag gtgatgtgaa tggcgaataa tcgtagactc gaagaaacat
     9241 tcaacgatgg ttggttaaag attttgacgc aaactaccaa aagaaatgaa ctaggaaaaa
     9301 agattggtgt agaagataca gaaatcactt ctttaaaatt tagaaatctt tccatgagag
     9361 atagtgatat aacagctatg gatgcgatgg gatcgaaatt aactaagaaa gtaaagactc
     9421 catttcatcc aatcgccaag aaatttaata aagatcaata ttttatcgta atcgatagta
     9481 tgcgttacaa cgttatctat gccgattacg ataattttta tatctatttt tatcttgaaa
     9541 gtgtgggtga atatggtgat tgataattct aaagaaaaag aacgtttaaa taagcaaatt
     9601 tctgctatca aaacttcctt agaagagcat tttggcctca aactctttca agactccgta
     9661 ggcgaggatg agctacctga tgattttaat tacttcattc tcgaaacagg agaaatagaa
     9721 atgatcactg agccaaaata tagcgtgggt caaaatctat atctaacttt ctattcagaa
     9781 aatagagaag atttaacagg agattcacta gatattattt cattgattca aaatcgttcg
     9841 attcgttttc agagaatgga tcccaatcat ttaaaactag agaaccaaga tcgctatatc
     9901 gatcaattgg tatttacgtt tagacgatta ttgaagagtg attgtcatgg ctaaaaatag
     9961 ttgggagcta aaaataaatg gacatgatga acttcttgtg cggatggaac gctattcaag
    10021 cgagagcgaa cgactgatta acgaagcatt gaaatcaaaa ggttcggcta ttgcagtgga
    10081 taggattaca gaaaaaattc ctgtttctga agcagattta agaagagggc accaacacgc
    10141 aaaaaatagt cgtccactta agactcaata cattaatttg ggtttcatca ttagacctac
    10201 aagaaaattt gagtatttaa aatatcctga tttggggata ggtacttcta aaagaaatca
    10261 gccggacgaa tttatgagaa gaggattagg tcttgcactt gatccaatta cagaacttct
    10321 gattcgtcaa ttcgataaat taaataaata ggaggaacaa caatggctaa aacaacaaca
    10381 gtaacaactt ttgacaatat aagtattaaa agaatttctt ttaattttaa gaatgcaaca
    10441 aatgcaattt caactgattg taatggacaa ttagatggcg aaacagaaat gcagagaatt
    10501 gtaaaaaaat gcggttcaac agaagtaaaa tcgaaatcta aaccaatcaa tatgacggta
    10561 acaattactg cacatgtacc gatggaagtt tatcgacgct tcaatgggtt gaaacaagat
    10621 gaacgtatta aatcgggaat ttactcttat ggtcctgatt ccgtagggga agatttctct
    10681 cttgctgcag agatcgtgga tgatttcgaa gaaaaaaata agctgattgg tatgttagca
    10741 tgcacttcga atacaggatt aacattctct attgaaaatg gtgcggatga agtagctgcg
    10801 ttagaactag aaacaaaagt tatgcaagat gaatttggta aattttatca tgaagcaatt
    10861 gttgcagaac ttgaagaaga cttaacagat caatggatga cgaatctatc tgctgatgtg
    10921 attaaaaaaa cttcagttgt gacaactacg gccactcaat cacagtaaaa aaaacggagg
    10981 tagcgaaatg aacgaagatt actcaaaaat tgaactaaac gatggaacaa ttttgaattt
    11041 agaacctaaa ctgaatatca agaaattatt gatgatcaat agagatttta acacagacga
    11101 gtttgcaaaa atgactgtgg gaaaaggatc catggatatt tctgttattc aaggtgcaaa
    11161 ggctgtgtat attgcttacc gccaagcgaa catgactgat tatatttcat tcgatgaatt
    11221 tatcgataaa tgggattttg atatggctac tgccagctat atttatcaat tgatgatgtt
    11281 caaacaagca cgcgatgctt atcaaaaaga atttgaaaaa gcaaataagg aaaaaaagct
    11341 tcaaaagtaa aaatgccaaa gctcttagtt gaaacgtggg tcgatgtcta ttcgatgttg
    11401 accgacgttt tttctatgcc ttcagatttg gttttaagcg atatctgttt agatgacatt
    11461 ttgcaaatgg cttacaacaa gagtgcttat gaaggatgga aaaactatgc aataaaccaa
    11521 tcccagaaaa actaaagaaa ggaggtaaaa aatggctaaa aagagaacag aagcagaagt
    11581 aacattcata gctaacgatg acggattgaa atctacgtta aaagaaatca gtgctgaatt
    11641 aactaaaaat agagcagaat taaaactaga acaagctcaa ttacaacaga ctggttctga
    11701 atcagacaag ttaggaagta aattatcttc tttagaaaag cagtatgaat tacaaagtca
    11761 aaaagttgaa gtaactagcc aacgtttagc caatgccaaa aaatattatg gagaaaattc
    11821 caccgaagtt cagaaacttg agagagaact gattaaccaa caaacagcgc aacaacgttt
    11881 gtcaaacgaa attgataaaa cgagtaatgc actagctcaa gcaaaaggcg aaatacagac
    11941 gtacgagtct acaatgcaac agttggatag tgaacaaaaa aatgttcaag ctagtgcttc
    12001 tctgattgaa tccgaataca aaaaatggca agcaactgct ggtcaatcag cttctgaatc
    12061 cgagaaatta gcgaaagccc aagaatatgt ttctcaacaa tctgaaaatg cagagaaaac
    12121 gatagatatc ctgagacgac agttagaagc tacacagtct gagtttggcg ctacatccac
    12181 agaagcaatg cagatggagg cgaagcttaa tgatgctgaa cgtgaatttg aagagttagg
    12241 acaagctgct aaaaatgtag atacaactaa cttggacgat atcggaagca aaatagatat
    12301 gaataatcta atggaagctt ctgacgtttt aagcgacatt ggcgataagc ttacagaatt
    12361 agggaaacaa gcagtggact ctgctaatag tgtaggtagt tcccagagta aaatacaagc
    12421 taattttggt ttgactaaac aagaggctga agaattaacg aatgtagcca gagacattta
    12481 ttataaaggt tttggagaat cgttagatca gtccacagat gcattgattt tggtaaagcg
    12541 taatttaggc gatttaaata atcaagattt acaaaatatc acggaacaag ctatggtcct
    12601 agaaaacacc atgggcgctg atatggatga aacgttacgt ggtgtaaatg gcttaatggt
    12661 caatttcggc ttgagcgctc aagatgcaat ggatttaatg gtttcgggta ctcaaaacgg
    12721 tttagataaa acgcacgaat taggcgacaa tatggcagaa tatagccaat tatggagtca
    12781 aatgggatat tcagctgatg aaacgttcgg aatgcttcaa aatggtttag atgcgggtgc
    12841 ttataacctt gataaagtca atgacttagt taaggaaatg ggaatatcgt taacagatgg
    12901 tcgatttgag caaaacatgg atatgtttag tgaaagtact agaaaagctt ttgaagagtg
    12961 gaaaaatggc ggaggaacac aaaaagacgt tattaattcc atgattcaag attttagcaa
    13021 tatggatggt caatacgacc aattaaataa agcttctaca atttggtctg cacttggcga
    13081 agataacgcg atgaaagttg tccaatcttt gactgatgtt aaccatacat ttgatgatgt
    13141 tagtggatct gcacaaaaaa tgaatgaaga ttctactact ccgttacaag aattaaacgg
    13201 aaaaatagct gaattaaagg attcattagc tcctataggc aacacaatca tagatgcact
    13261 cgaaccagta attgattttc taggaaagat ggctgatgcg tttaataatc ttccacaacc
    13321 agtacaggat tatgccgtag cgattggcgg attgactgct gcatttactt tattaatgcc
    13381 aataatagtt ggcttcatgg ctctaggtgg tcctactaca ttaataatag gagcagttat
    13441 tactgttatt gctggagtta tagcaattat aaaaaactgg ggcgcaatta ctgactggtt
    13501 taagggaata tggagtaaat tcactgattg gttgggtggt acttgggaaa gtataaaaga
    13561 aggtgcctca tcagtttggg atggagttaa agaaacctgg tctggatttg tagattgggt
    13621 tcaagatatt tggcaaggag tttctgattg gtttggagag ctatggagcg gattagttga
    13681 aggagcttcc aacatctggc aaggagtcca agagacttgg caagcattcg ttgattgggt
    13741 ttcaaatatt tggaacggag tcaaagaagt atggtcgatt atttgggcag acattgtagg
    13801 aattgttcaa ataccatgga ccttaataac gtcattgatt caagccggta ttaatattat
    13861 cgtgggtatt tttgatgtag ctggacagtt attaggcgca gcttggcaag ctgtttggac
    13921 acctatttct gatttcctta aaaacacttg ggatactatg acacaatggg taagcatcgc
    13981 ttggaatgga attgtaacta cattccatac tatatttgat ccagtagtgg catggtggaa
    14041 tggtatatgg acagctatta gtactacggc ttcaaatatt tggaattcaa ttagtgcaac
    14101 agcttctagt atttggaaca gtatcaagaa tacaatcact agcttggtac aagcagctgc
    14161 tacagtaatt caaaatattt ggtcaactgt atctagttgg ttaggtggaa tttggaattc
    14221 aatcagctct acagcatcaa atatctggaa tagcgtgact agtagtataa gcaatgctat
    14281 aaacgcagct aaaagtgcca ttcaaagtgt ttggaatagt atatcttcgt ggatcagcgg
    14341 aatttggaac ggtatcaaaa acactgcttt gaatctttgg aatggaatta caagcactat
    14401 tagctctaaa gtaaacgatg gaaaaaatgc aatttcaagc ggttggtcca atctaacagg
    14461 tattgtttcc gacatattca ataatgttaa aagtacaatt gctaacattt gggaaggtat
    14521 caaaaagact gttagcgctc caattgattg gatcagagat aaaatcagta gtatctttga
    14581 taatttgaat atttctatac cacatattcc gttaccacat tttaaattga gcggagaatt
    14641 caatccattg aaggggaaaa tcccaacgtt gggtgttgat tggtatgcga aaggtagtgt
    14701 gtttaattct ccgaatatta tcggtgtcgg cgaagcagga cctgaagcag ttttaccttt
    14761 gaaaagatct gtgctgcaag aaattggtga tcgtatcttg agtagcacat cagtttcatc
    14821 tagggcacaa acgattcaac ctgtgaataa ctacgaattc aatttcacaa ttgatggtaa
    14881 cgcagatgag gttactatga agcaaacaac tcaacaaatc attgatagca ttacaaaagt
    14941 tcaaaatgat aatgcttcgg catggcgtta aacaggagag tatttctcct gtttttttag
    15001 tattaaaaag gatgtgaaaa aatgactgat tgtatacatt ctataatcga tggatttcct
    15061 gattatttgc ataaattggc tttagctgaa agaccaacca taccttctcc aaaaagacag
    15121 agagttgaaa cttctgtttt aggaaggtta ggtggcttag tacaagatta ctcgtttgaa
    15181 gacatgtcgt ttacattgca ctataactat ttagaggatg tggaagacca tcaagcgttc
    15241 aagcaatcgt tttatatcat gcgtcattgg ttaaattatg caaagaaatt agaattctct
    15301 gatgatccca acgtctatta tgttatccag actatcgata ttggggatgc agaaaacgat
    15361 attgttgaat ggggagagtt cgatgtaaat attactgcga aaccattcgc aagagttcaa
    15421 gaagatgtac ctataaccgt agataaacca cagtcattta acttgctgaa taatagttta
    15481 gaagaaagtt ttccaaagat tatcatcact ccttcagcta cttcatgcca gttcatctta
    15541 aatgattatg tgtttagttt tgaaggctta gtagtaggaa ctgacgtagt cattgatagt
    15601 gatttgatgc tttgctacga agagcaatcg gacggagata ttttagatcg gtccaacaaa
    15661 atgaagacca tgcaatatcc gacattgcaa gtggatatta attattttaa ttgtactggt
    15721 ttgagcaaaa tacaaattta tcgtaatggg ttaaggtagg tgaaatagat gatcgataat
    15781 ttaataacta tttacgataa aaacgacgcg aataatttag ctgaacattt atatgatacg
    15841 caaggtttag gcgctttgtc agactggtta acagctactg ttagcaataa attaaacgga
    15901 gccgagatat ttcagggtac ttatccaata agcggaacta atgcagattt gattgtagaa
    15961 ggacgtatta ttcagtgtta tgtagatgaa aatcgagcaa aacagcgtct acggatttat
    16021 tatgcaaaga cttctgtaat aggcaatacg atagaagtaa aagctgaacc tattttcaat
    16081 gatataagaa aatcggtgtt gaataaatat gacagtggaa cagaaaagat cactgctagt
    16141 caggcatggc aaaacgcaaa aactttagcg aaaccagtta ttccttcaca gttttctttc
    16201 tcgtcattag tagatacgct tgctaatgtg aagatagaaa aggcgaattt tttagaattc
    16261 tttggtggaa aagagggatc tattctagat cgatttcatg gggaatttct aaaagataat
    16321 aacacattac gtcatgaaaa aaggctaggc acggatcata aaatcaaagc gatttatact
    16381 aaaaacttaa ctggtcttga cttagagatt gatgctcaaa gtgttttagt tggagtttat
    16441 ccattcatta gcagctcttc agaaggagaa gacgagatca ctctaccaga agaagttatt
    16501 ttcacggatt acgtggatga ttatcctgct ggatatgttt cttttgttga ttttaaagac
    16561 aaagcgactg atgtagccac attaagggaa gctgctaaag actggttgaa aacaaacata
    16621 gataaacaaa aaccacaagt gagtggttcg attgaattag taccattgag gcatcaaaga
    16681 ggctatgaaa aatttgttga tctagaaaaa gtttcgatgg gtgacggagt agatgtgtat
    16741 catccacagt taaaagtgaa tatgtcagcg agaattgtgg aatatacgtt taatgttcta
    16801 accaattcat acgataaatt agttgtagga aacgtcaaaa caaacttctt agaaaacaca
    16861 gagaataatg tcagcaattt gattaatgat gccattgatc aattgaaaaa cggtggcgaa
    16921 atcagtgatt tactcaatga tattgtagat catcaaactg atatgattac tggtcaaaat
    16981 ggtggttatg ttttattaga tcctaaagaa gcgcctagtc gtattttgat tatggacaca
    17041 ccagataaga ataccgcaag gaatgtttta caaatcaaca atgctggtat tggtttttct
    17101 aaaactggca ttaatggaac atatgaaacg gcatggacgt tagatggcgg attcaatgcc
    17161 tcgtttatta cggctggtga aatagtagga attactatta gaggtactac attaattagt
    17221 gatggtgctg attatagaac aagtattgct aatggcaaaa tgacttggta ctcaaaaaaa
    17281 gttaacaaag atattatgga gctagaagca cgtgattatg taagtgctga tgccggtatt
    17341 gtatcataca ccatgaaaac tggtggtggt ttcatgatta gaaatccaca gggtaacttg
    17401 gtttttagta cgtgggataa tggtaataac agaccgtttc tatcttttgg tgcgcccaat
    17461 ttcaggtata gcaatgctag ttatgtaact tctggcgacg gtagttcttt aagcattaat
    17521 ggtagtgcgg gtaactcatg ggaatttaag gtagctggta ggactatgaa atttactagt
    17581 gatggtatgc taacgttacc aggttgtttt tttggttcat gggaagatgg gaaacttgct
    17641 aggtttgaac aatcaacggt acaagtatat aaagatttta ctgttagagg tactaaaaac
    17701 tcaactgtac caacagaaca ttatggacaa cgactattga acgcttatga aactccagaa
    17761 tattatttcg ctgattatgg ggaagccgtt acaggtgaca atggtaaagt tcgtgttgat
    17821 attgacccca tgtttgctga aacagtaaat ctaagtcggt atatgacaca tgtgacacct
    17881 acagaactag ttttgtgtgc tgttactcat gaagatattg accatttcat cattgaaact
    17941 agtaagccaa acgtattagt tagatggaat ttagtggcac accgtctagg gtatgaagat
    18001 attagattaa aagaggatac agcatatgat agcacagtgc ttgaccaaaa acgtttttaa
    18061 aacgaagaca aggaggtata taaatggcta gcagtttata taatttggct ttagatttca
    18121 gcaaagaatt aaactacacc aaagctatta tggctcgtca aggtgataaa gggattacgg
    18181 tgacggttaa accgtttcta aatggcttgc agatggatac gagtggcgga acatttactt
    18241 taaaaggaac aacaccatct aaccgttacg tagataatgt tgcaactagt gtaactagtg
    18301 aagaagtcac gttttctctt gatggcacat ttatgagtga agcaggatat tataaacact
    18361 gctacgtaga atatagaaaa gacaatcaaa ttttaacaac gcaagatatc atttttttct
    18421 cactaggagt gtctgacatt tcgcaaggcc aagccgatga atatgtttcg caattagaag
    18481 agttgattcg aaagtacaac gaaacttttg atgcttttat ggctgaaatc aaaggtagag
    18541 tggatagctt aaatcaacag attactgatt taactggtca agctaaaacg ctacaagaca
    18601 agttagatgc tctgaaagaa gaaatttcta agttaggtaa cttacaagtg atgtacagta
    18661 acagcatcga cttcgggaat tatgatta
//
LOCUS       DASHHB010000054        18584 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.056, whole genome shotgun sequence.
ACCESSION   DASHHB010000054 DASHHB010000000
VERSION     DASHHB010000054.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18584)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18584)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18584
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.056"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(367..933)
                     /locus_tag="U2K71_002152"
     CDS             complement(367..933)
                     /locus_tag="U2K71_002152"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM7316185.1"
                     /translation="MLQTALHFSHTLLQEILQPGDHVVDATMGNGHDTVFLAEHIGKT
                     GHVYSFDIQQQAIDATRERLEQRQLEERVSLFLQGHETLGEVIAEQQNLKAGIFNLGY
                     LPKSDKAIITMPETTRTAMEEILKRLVPRGRLILVVYYGHEGGEKELDMVDSFCSKLP
                     QETYNVLNYRFINQKNQPPILYCIEKKR"
     gene            complement(933..1889)
                     /locus_tag="U2K71_002153"
     CDS             complement(933..1889)
                     /locus_tag="U2K71_002153"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390904.1"
                     /note="This family includes YhcC from E. coli K-12, an
                     uncharacterized radical SAM protein; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01212 family radical SAM protein"
                     /protein_id="HEM7316186.1"
                     /translation="MTFPYSEGNKRYHSWNYALRNEFGGKIFKVPIDGGFDCPNRDGT
                     VAHGGCTFCSVSGSGDMIVAPEDPLPIQFQKEIDMMHQKWPHVQQYIVYFQNFTNTHA
                     PVEIIKERFEQVVNLPGVVGLSVGTRPDCLPDEVVDYLAELNERMYLWVELGLQTTFE
                     ETSKLINRAHDYQTYLDGVAKLRKHNIRVCTHLINGLPGESLEMMKENVRRTILDSDI
                     QGIKLHLMHLMRKTRMLRDYHEGRLQLMSRPDYVNVICDQLEMIPKEIIIHRLTGDAP
                     WDSLIGPMWSLKKWEVLNAIDEELLRRDSFQGKYDVRKKVSV"
     gene            complement(1889..2545)
                     /locus_tag="U2K71_002154"
     CDS             complement(1889..2545)
                     /locus_tag="U2K71_002154"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HEM7316187.1"
                     /translation="MKYKTSFQVAGSFFLVLFFILGWIVKFHLSWLSGFDQWFTGIVR
                     ASYPDLNSYQLFMTKFGNPLTVIILFACAALWMWYKKKRAETFWFVSGFIVIAGIINP
                     VIKLFFMRERPSLAHLVVETSYSFPSGHAAGSMILYGSLLFLLPAFVQKRTLRTLLQL
                     LMMFIIVSVGISRIYLGVHFPTDIIGGYLLSLSWLCLTYPVYREKRKQIDNSYRKKDF
                     "
     gene            2915..4933
                     /locus_tag="U2K71_002155"
     CDS             2915..4933
                     /locus_tag="U2K71_002155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HEM7316188.1"
                     /translation="MENIARKERRRLEQTKRFRKAKRGAAIVGTAMVGCSVAAPLVQP
                     VPVQADETPVQFGARINTAAFITEIATYAQPIAQANDLYASVMIAQAVVESGWGSSAL
                     SQAPYYNLFGIKGSYQGQTVYMDTLEFLNNQWVNKKEPFRQYPSFAESFNDNAYVLRN
                     TSFGNGYYYAGTWKSNTKSYTDATACLTGRYATDPGYAGKLNNIIATYGLTQYDTPAS
                     GNAGGGATTGGNNTGSAGSTGGSSNSGNTNSGSATSTTYTVQSGDSVWGISNKFGITM
                     DQLIQWNNIQNNFIYPGQKLTIKGSQENGSSTNNSGNNTNSSGNAGTSNGGQTTGAKY
                     TVQSGDSVWKISNDHGITMAQLIEWNNIKNNFVYPGQQLIVSNGGSSNAGANTGSNTG
                     NTSGSSSSSSSSSNTASGTKYTVKAGDSVWSVSNKYGITMAQLIQWNNIQNNFIYPGQ
                     QLTVSNSGSTNSTSGNTGSSTNTNNSSSVNTGSSSSQGTYTVKAGESVWSVANKNGIT
                     MDQLIEWNNIKNNFIYPGQQLIVKGGTSANTNTGSTTSAAKPNTPNTSATTSTSSTGN
                     TMYTVKAGESVWSVANKHHITMDQLIEWNNIKNNFIYPGQQVIVKKGSSQSTNQQSVT
                     GSKTYTVKSGESVWSVADSHGITMAQLIEWNNIKNNFIYPGQTLIVAK"
     misc_binding    5003..5209
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            5360..7774
                     /locus_tag="U2K71_002156"
     CDS             5360..7774
                     /locus_tag="U2K71_002156"
                     /EC_number="6.1.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019722570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucine--tRNA ligase"
                     /protein_id="HEM7316189.1"
                     /translation="MSYNHKEIEKKWQKYWAKTNTFNTHDDPEKPKFYALDMFPYPSG
                     QGLHVGHPEGYTATDILSRVKRSQGYNVLHPMGWDAFGLPAEQYALDTGNDPAEFTQK
                     NIETFRRQINSLGFSYDWNREVNTTDPEYYKWTQWIFTKLYEKGLAYEAEVAVNWVPE
                     LGTVISNEEVIDGKSERGGYDVVRKPMRQWMLKITAYADRLLDDLELVDWPESIKEMQ
                     RNWIGRSVGANIEFKVAGTDKSYTVFTTRPDTLFGATYSVLAPELDLVREITTPEQKA
                     AVEAYIEETAKKSDLKRTDLAKEKTGVFTGAYAINPVNGKEIPIWIADYVLSSYGTGA
                     IMAVPAHDERDFEFAQTFGLEILPVIEGGDVEKAAYTEDGTHINSEFLDGMNKQEAID
                     KMNEWLEENGVGKKEVSYRLRDWLFSRQRYWGEPIPIIHWEDGTVTAVPEEELPLRLP
                     KTKNIKPSGTGESPLANIEEWVNVVDPVTGKKGRRETNTMPQWAGSSWYYLRYIDPHN
                     KKELADYEKLKRWLPVDIYIGGAEHAVLHLLYARFWHKFLYDIGVVPTKEPFQKLYNQ
                     GMILGENNEKMSKSRGNVVNPDDVVETYGADTLRMYEMFMGPLDASIAWSENGLEGSR
                     KFLDRVWRLIVDENNKMRDRITTLNDGKLDKVYHQTVKKVTEDYENLHFNTAISQLMV
                     FINEAYKVDALPYEYIEGFVQLLAPIAPHMGEELWAILGNDGGISYAPWPTYDEAALV
                     EDEVEVVFQVNGKVRAKSNVPRDLGKDELEKAALANEIVQEYIEGKTVRKVIAVPNKL
                     VNIVAN"
     gene            8122..9675
                     /locus_tag="U2K71_002157"
     CDS             8122..9675
                     /locus_tag="U2K71_002157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HEM7316190.1"
                     /translation="MDQELFIIEVLSAKQKIIEKAKKRAKRIMFKLLSLVFFSGLLFF
                     GAVSFLYRHMSAVVRSAEGMQKLNNLYHFSWGVGGITIVVLIVFLLLIWLRYHQLSRH
                     FDQWLSQRAQQFLHNKPTSQDDDYLYFEKTSTSQAFRIQKSDCQLLLRSLDDLPIYLG
                     ESKSKFGMNTFHIFLLENEPRWEKSQSSPGGYLTTKMSTLVTGFFLILLAGTIYYGRY
                     TQPKMSSHINAASHMTMNSTNDTHIQKTNEGLLADPKQKVNLTTQKINDLKLDTETHS
                     LYMTTDSGANWVFVPIDSQWLRFGEYTLTTGLIPEGYWMDNTYDISADFSWFIYSSGD
                     DVRLLTSRDNGKTWQTSIITQSVQPIRYRKAVFYGNDGLFICTSASGMSAETLYIYTT
                     MDGGITWIKSGSTMINQPVQNVSFVTTSLGFVSTRESLFYTNNGGRSFKESVLSIPEN
                     YQTGGIDLFQSPNEVTQISANTLETKFYLLKKGPIDAGKMFACLFRSTDNGETWQFVQ
                     QLSQVEQGD"
     gene            complement(10056..11840)
                     /locus_tag="U2K71_002158"
     CDS             complement(10056..11840)
                     /locus_tag="U2K71_002158"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7316191.1"
                     /translation="MEKKKTLNTRALIRFWKMIKPEHKYFYGLFLCSLFGNLLVVAMP
                     LIMGIGIDDLLSEIRKTGIGQLSFSDIKQALLFPVLLLIGCSVLSSITSFIQERTMAA
                     LSEKITLRVRKEITKKFKTLPMSFFDNHQVGDIISRTTTGLNQLSQVLLTGINQFFTS
                     VVTIAAAGLMLFYIESKLTLLVLLLIVGSSIVTTKIANKNKQISEQNQYELGVLNNRM
                     EEILAGNLVVKTFNQQAHAEAAIHEVNKKQYNAFKRAQFMNCAIYPAIRFINQLAFII
                     SAVFGAMLVLSGGVTIGFLQAYLQYINQISEPISTASYVINSIQSAMAAIDRIFEILD
                     ETDEIPDLPDARLLEQPKGKIAFKNVQFGYTSDKILMNQVDFSVKPKQTVAIVGPTGA
                     GKTTLVNLLMRFYELNKGKIEFDGLDITKLSRGELRRSFGMVLQNTWLFEGTVADNIA
                     YGKMDATREQIIEAAEIAQCDHFIRTLPDGYDTIISSENGTLSQGQQQLLTIARVILA
                     NPPVVILDEATSSVDTRTEALIQQAMEAVTKDRTSFVIAHRLSTIENADMILVMKNGD
                     IVEKGTHSELLQSPTLYASLYNSQFQIA"
     gene            complement(11855..13576)
                     /locus_tag="U2K71_002159"
     CDS             complement(11855..13576)
                     /locus_tag="U2K71_002159"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7316192.1"
                     /translation="MSILKTFLQKNKGLALLTFVMICLEIAGTLGVPKLVAKLIDTGI
                     ASGEASVIKKIGVEMLLVAFLGTIAAVASSYFSALASARFGFQTREKYFRKFQQLSMK
                     DISRFSSGSLLTRMTNDVDNVQQMIVLFCQMILPAPVICVFTILMMFRYSLLLTWVTL
                     ISVVFYVWIVYRLMKRGAPLSLSIQPKMDRITITLREFFTGINMIRAFDNQSYEEKRT
                     NKSFADYANQMIKVNRIFAWVTPIAFLLMGIVYASILWFGGNLVAEGALQIGVVTAVV
                     EYSMLTLAYLMIAAMVLVIIPKSFASLRRIEEILQAEIEIEEETIKTVAEPTFDQTNA
                     AAFEHVTFKYTEISDPVLEDIDFTIPKGKTTAIVGGTGSGKSSLAKLLLRLSDPTIGK
                     VTLGGIPLTQMTQEQIRSHISYVPQKAFLFSGTILSNLQMGNEYASTEELSKAIKIAQ
                     LEELVDTLPDGLDSFVAQGGSNYSGGQKQRICIARALIKPAEIYVFDDSFSALDYKTD
                     AALRAALHKHMSEKTLVIVAQRLSTIQQADNIIVLDEGKIVGQGTHEELLSNCQTYQE
                     FAKSQGI"
     gene            13676..14437
                     /locus_tag="U2K71_002160"
     CDS             13676..14437
                     /locus_tag="U2K71_002160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM7316193.1"
                     /translation="MKKMYTIGQVSELFDLPKSTIRYWDEQGLIRSSRQEENDYRLFD
                     IDDIFMLYDIVFYRKLDIPIKQMKNLYGKTLTELYETLDETERRIHKELVVMKQKQKE
                     IREQKKQLKLMIDTNEEEFPVEEIPFDCMISTEFEDIVEIKKFLPNYSSFGMMSMPAS
                     SSQTMYGFFIDPSEVHLFTQDVIWEKKDTAVYRRFLLKSEMNHAERNNILEIRERMYE
                     KGWKTGEVIGQYLLTNTDENNIRTEYYHAWIEMKK"
     gene            15404..15672
                     /locus_tag="U2K71_002161"
                     /pseudo
     CDS             15404..15672
                     /locus_tag="U2K71_002161"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013720767.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS607 family transposase"
     gene            16344..>18584
                     /locus_tag="U2K71_002162"
     CDS             16344..>18584
                     /locus_tag="U2K71_002162"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316194.1"
                     /translation="MRNKFHKLLSMFLVMLIVINQFGNITVLAESLAEQPERSLTIEV
                     DQEEAALGEQFQLRVRGTPDLLNALVIDKSEDLIEGEVKQLNDYERLITYSSQHSGKY
                     ELTARSGDIQSNTVSIEISVPEESTSESFNVPEESTSESFNIPEESLSESFNEARSSE
                     PSITQESQIERQEELLNYDIGISAERPTLPSRHTQTLKGYIQGGAGDILEEYGEMVVR
                     VPRASIGLIEFIRVEVADDSPLKPLAFYQEGTDVVFPFTFDYEGLNEAAQSYTFSITF
                     EVAFFQSGGTEPNNDFQVSLTYAERAANTEFSITPIPSSRPIFSKLYYGSREIDSQGN
                     LGNLLIDGQDVTNSNKFILQVNYEGSRKDGVVVTDVLPQGTTLAPNPTTWSSTGNREP
                     ISGGVRIAEVTNPGEANVRTHYVSEEFVDKVSFNKETNTLVVDFGNLAEGQSYFIEYA
                     LEVTDVDLFNEGRQEDGFSRNRASMTYNNQVMSRDYRLRIPESNLPAISLRKSVDKNV
                     INLNENELTYTLHVSMFSGTRNTLVIEDVLEEGMEFVSFLSDIPSFIDAEVNNGIVRF
                     SANNTIGGPTSFTLSFKVNVEAYAVGDQILNFATLVNSDNRLNTSTVRTRKIDGRIKV
                     IKTDENGQRLAGAKFEILSSDSQVIQEGITAENGEFLSEPLPVGSYQVRETEAPEGYL
                     LDATVHDVFINEYSIEEVTLTIENILDTGGVELIKHAEGSGEVLQGAVFELQNREGET
                     LQTGLTT"
BASE COUNT         6108 a         3301 c         3745 g         5430 t
ORIGIN      
        1 ggggcataat ttgactgata ttaagttagc tattgatata tgaatgacat acaaaaatat
       61 aaaatctttt aaaaatgctt atatatcaat gtttttgcca ttcttttata tatctgttat
      121 atgtcaaaaa aatgtgttca atttcaattt gtatttgaat aaagaggttg tgaaaaagct
      181 gtcctgcatc aagtaataag aacagccaat caaaaacagc ttctcatgtt tttgatttgg
      241 ttggacttat taccgcagat ataagctttt gagcaccgtg aataaagagg ttatgaaatc
      301 aacgctctaa cctgagtatt agagaaaaaa acgcaaaaca tatcatttat gttttgcgtt
      361 ttttatttat ctttttttct caatacaata taaaataggc ggctggttct tttggttgat
      421 aaaccgataa ttcagcacat tatatgtttc ctgtggcagt ttggaacaaa aagaatcaac
      481 catatccagt tctttttctc caccttcatg accatagtag acgacaagga tcaatcgtcc
      541 gcgaggcaca agtcgtttca atatttcttc catagctgtg cgtgtagtct ctggcatagt
      601 gatgattgct ttatcacttt ttgggagata ccctagattg aaaatccctg ctttgagatt
      661 ttgttgttca gctatcactt ctcctaacgt ttcgtggcct tgtagaaaca atgagacacg
      721 ttcttctaac tgacgctgtt ctagcctttc acgagtagca tcgattgctt gttgctggat
      781 atcaaaacta tagacatgac cagtttttcc gatgtgttct gctagaaaaa ccgtatcgtg
      841 gccattgccc atcgttgcat ctacgacgtg atcccctggt tgcaagattt cttgcagtaa
      901 tgtatggctg aaatgcaaag cagtttgcaa catcagacgc tcaccttttt ccgtacatca
      961 tattttcctt ggaagctatc tctgcgaaga agttcttcat caattgcatt caagacttcc
     1021 cattttttca agctccacat tggtccaatc aaggaatccc acggcgcatc ccctgtcaaa
     1081 cgatggatga tgatctcttt tggtatcatt tccagctggt cgcaaatgac attgacatag
     1141 tcaggacggc tcattaattg aaggcgacct tcatgatagt cccgcaacat tctcgttttg
     1201 cgcatcagat gcatcaaatg gagcttgatt ccttggatat ctgaatctaa aatggtgcgt
     1261 ctgacatttt ctttcatcat ttctaaactt tcgcctggta atccgttgat caaatgcgta
     1321 caaacacgga tattatgttt gcgtaattta gccacgccat ctaaataagt ttgataatca
     1381 tgtgctcggt tgatcagttt tgaagtttct tcaaaagttg tttgcaatcc aagttctacc
     1441 cataaataca tccgctcgtt cagttcagca aggtaatcaa ctacttcatc tggcagacaa
     1501 tccggtcgtg taccgactga taatcctacg acacctggta aattgactac ttgctcaaat
     1561 cgttctttga ttatttcaac tggcgcatgg gtgtttgtaa aattttggaa ataaacgatg
     1621 tattgttgga catgaggcca tttttgatgc atcatgtcga tttctttctg gaattggatt
     1681 ggaagagggt cttcaggtgc aacgatcatg tctcccgaac cagatacact gcaaaaagta
     1741 caaccgccat gggcaacagt cccatctcga ttcggacagt caaatcctcc atcgatcggc
     1801 accttgaaaa ttttcccgcc aaactcattt cgtaaagcgt aattccaaga atgatatctt
     1861 ttatttcctt ctgaataagg aaaagtcatt aaaagtcctt ctttctgtaa gagttatcta
     1921 tttgttttct tttttctcta tacactggat aagtcaggca cagccaagaa agactgagta
     1981 agtaaccgcc gatgatatca gtaggaaaat gaacgcccag ataaatgcgg cttatcccga
     2041 cacttactat aataaacatc atcagcaatt gaagcaatgt gcgtaaggtt cttttttgaa
     2101 caaaagctgg aagcagaaat agtaaagaac cgtaaagaat catactgcct gctgcatgtc
     2161 cactagggaa actgtaactt gtttcaacta ctaagtgcgc caatgaagga cgctctcgca
     2221 tgaaaaatag tttgatcacc gggttgatga ttccagcaat tacaataaat ccagaaacga
     2281 accaaaatgt ttctgccctt tttttcttat accacatcca taaagccgca caagcaaata
     2341 aaatgatgac ggtaagagga ttaccgaatt tagtcataaa gagttggtag ctgttcagat
     2401 caggataaga agctctgaca atgcctgtaa accactgatc gaaaccagaa agccacgaca
     2461 gatggaactt tacgatccag cccaaaatga aaaacaggac aagaaagaag ctaccagcca
     2521 cctgaaatga tgttttatat ttcatacaat tcctcctttg aagcaatgac ttattatagc
     2581 atttttggtc cagcgaaaaa aacggtaaag agataaaacc aatgtatgat tcctctttga
     2641 ataaccaata ttcttataaa aaaaataact gtaatcggaa agacattttt tggtaatgca
     2701 aatgaaatgt tagggaaata aaatcagtgt ttttcgaaat ccttttaaac aagaacgtag
     2761 acgtaggaag aacttgaaaa tgaacagaag ttgttcatta cggtaaatta caatatgatt
     2821 acaaaaaaca acaaagctct taaataactt ttaataaata ttaagagaag ttataattat
     2881 taataataga taaattgaag ttggaggata gaagatggaa aacattgcaa gaaaagaacg
     2941 gagacgttta gaacagacca aacgttttcg aaaagcaaaa cgaggggcag caatcgtggg
     3001 aactgcgatg gtaggctgtt ctgtcgctgc cccattagta cagccggttc ctgttcaagc
     3061 ggatgaaaca cctgttcagt tcggcgcaag aatcaataca gcggcattta tcacagaaat
     3121 tgctacttat gctcaaccaa tcgctcaagc caacgatcta tatgcatcag tgatgatcgc
     3181 acaagcagtt gtagaaagcg gttggggcag tagtgcgtta tctcaagcac cttattataa
     3241 tctttttggg ataaaaggca gctatcaagg acaaaccgtc tacatggata cgcttgaatt
     3301 tttaaataat caatgggtaa ataaaaagga gcctttccga caatatcctt cttttgctga
     3361 atctttcaat gacaatgcct atgtacttag aaatacttca tttggcaacg gttattacta
     3421 tgccggtact tggaagagca acacgaaatc ctatacagat gcgacagctt gtttgacagg
     3481 gcgttatgcg actgatcctg gttacgcagg caaattaaat aatatcatcg caacttacgg
     3541 attgacccaa tatgatacac cagctagcgg aaacgcaggc ggtggagcga ctacaggtgg
     3601 caataatact ggaagtgcag gtagtactgg cggttcaagc aattctggta atacaaactc
     3661 aggttcagca acttcgacca cttatactgt ccaatctggt gattctgttt ggggaatctc
     3721 aaataaattc ggtatcacaa tggatcaatt gatccaatgg aacaacatcc aaaacaattt
     3781 catttatcca ggacaaaaac tgacaatcaa aggtagccag gaaaatggct cttcgacgaa
     3841 taattccggt aacaatacca attcttcagg aaatgctggt acatcaaacg gaggacaaac
     3901 aactggtgcg aaatacactg ttcaatctgg tgattctgtt tggaagatct caaatgatca
     3961 cggaattacg atggctcaat tgattgaatg gaacaatatc aagaacaatt tcgtttatcc
     4021 aggacaacag ctaatcgtat caaatggcgg ttcttctaat gctggagcaa atactggctc
     4081 aaatactggg aatacaagtg gctctagttc gtccagctca agttcttcta atacagcttc
     4141 aggaacgaaa tatacagtta aagccggaga ctctgtttgg agcgtctcaa ataaatacgg
     4201 aattacgatg gctcagctaa tccaatggaa caatatccaa aataacttta tctacccagg
     4261 acaacaactg actgtttcaa acagtggatc aacaaacagc acgtcaggta atacaggttc
     4321 gtcaaccaat acaaataaca gcagttcagt taatactggc agcagttcgt ctcaaggcac
     4381 ttataccgtg aaagcaggtg aatctgtttg gagcgtggca aataaaaacg gcatcacgat
     4441 ggatcagtta atcgaatgga ataatatcaa aaacaacttc atttatccag gacagcaatt
     4501 gatcgttaaa ggtggaactt cggcaaatac aaatacaggt tcaactactt ctgcagcgaa
     4561 accaaataca ccgaatacgt ctgctacgac aagtacatca tccactggta atacgatgta
     4621 taccgtgaaa gcgggcgagt cagtttggag cgtagcgaac aaacatcata tcacgatgga
     4681 tcagttaatc gaatggaata atatcaaaaa taatttcatc tatccaggac agcaagtgat
     4741 tgtgaagaaa ggttcatctc aatcaacaaa ccaacaatca gttactggtt cgaaaaccta
     4801 tactgtgaaa tctggagaat ctgtttggag tgttgctgac agtcatggaa ttacaatggc
     4861 tcaactaatc gaatggaaca atattaagaa caactttatt tatccaggtc aaacgttgat
     4921 tgtagctaaa taaaatagct gttcgaagaa ataacgattg aattccacaa aaatatttgt
     4981 tactataagg ctacttaaat atgacaacac ttgaaaagga agtagtaaaa agactgaagt
     5041 ttcagagagc gcatggctgg tgagaatgtg cacggatgct tttgaactcg cctttgagtt
     5101 gcccttgctg aatacccgag taagcaagcg gcggtgacgg ccgttacaac gttcgaggcc
     5161 ttttcactaa ggtaaaatta ggtggtaccg cgtagattac gtcctataaa aagagcaatc
     5221 tttttatagg atttttttat ttcttttgtg aagtttgaga aaacaaagca aaaggaaata
     5281 aaaataaaca gattctgccg gttttttcgt tatatttata atagaaacaa gtgttttcaa
     5341 tagaacagga ggacagcaaa tgagttacaa tcataaagaa attgagaaaa aatggcaaaa
     5401 atactgggca aaaacgaata ctttcaacac ccatgatgat cctgaaaagc caaaatttta
     5461 cgcactggat atgtttccct atccatccgg tcaagggcta catgtagggc atcctgaagg
     5521 ttatacggca acggatatcc tttcacgtgt gaaaagaagt caaggataca acgtgctgca
     5581 tccaatgggg tgggatgcat tcggtctgcc tgctgaacaa tatgcgctag atacaggaaa
     5641 cgatccagct gagtttaccc aaaaaaatat cgaaacattc cgacgccaaa tcaattcttt
     5701 aggatttagt tatgactgga atcgcgaagt gaatacaact gatccggagt attataaatg
     5761 gacacaatgg atctttacga agctttatga aaaaggtcta gcttatgaag cagaagtagc
     5821 ggtaaactgg gtacctgaat taggaacagt tatttcaaat gaagaagtga tcgatgggaa
     5881 aagtgaacgt ggtggctatg atgtagtcag aaaaccaatg cgccaatgga tgctgaaaat
     5941 cactgcttat gctgatcgct tattggatga tctagaatta gttgattggc cagaaagcat
     6001 taaagaaatg caaagaaatt ggatcggtcg ttcagtagga gcaaatattg aatttaaagt
     6061 ggctggtacg gataaatcct ataccgtatt tacgacacga ccagatactc tattcggtgc
     6121 aacttactcg gttcttgcac ctgaactaga tcttgttaga gaaattacga cccctgaaca
     6181 aaaagctgct gtagaagctt atatcgaaga gacagctaaa aaatcagatc taaagagaac
     6241 ggatcttgca aaagaaaaaa caggagtatt tactggtgct tatgcaatca atccagttaa
     6301 tggcaaggaa atcccaatct ggatcgccga ttatgtatta tcttcatacg gcacaggagc
     6361 catcatggct gttccagcac atgatgaacg tgactttgaa ttcgctcaaa ctttcggact
     6421 agaaattctt ccagttatcg aaggtggaga tgttgaaaaa gctgcttaca cagaagatgg
     6481 tacgcacatc aactctgaat ttctagacgg catgaacaaa caagaagcaa tcgataaaat
     6541 gaatgaatgg ctagaagaaa atggcgtagg gaaaaaagaa gtcagctatc gcttacgtga
     6601 ctggctattc tcccgtcaac gttactgggg tgaaccaatc ccaatcatcc attgggagga
     6661 cggaacagtc acagctgtcc cagaagaaga gctaccatta cggttgccta agacaaaaaa
     6721 catcaagcca agcggtactg gagaatctcc attagcaaat atcgaggagt gggtcaatgt
     6781 cgttgatcct gtaacaggta aaaaaggccg tcgcgaaacg aatacaatgc cacaatgggc
     6841 aggaagctca tggtattacc tgcgttatat cgaccctcat aacaaaaaag aactagcaga
     6901 ttatgaaaag ttgaagcgtt ggctaccagt tgacatctac attggtggag cagaacatgc
     6961 ggtacttcat ttactatatg cacgattctg gcataaattc ttatacgata tcggtgttgt
     7021 tcctacaaaa gaaccattcc aaaaactata caatcaagga atgatcttag gcgaaaacaa
     7081 cgaaaaaatg tcaaaatctc gtggtaatgt agtcaatcct gatgatgtgg tcgaaactta
     7141 cggtgcagat acactgcgca tgtacgaaat gttcatgggg ccattagatg cttcgattgc
     7201 ttggagcgaa aatggcttag aaggaagccg taaattcttg gatcgtgtat ggcgtttgat
     7261 cgttgatgag aacaataaaa tgcgtgaccg tatcactaca ttgaatgatg gcaagcttga
     7321 taaagtgtat catcaaacag tgaaaaaagt gacagaagac tatgaaaatc ttcatttcaa
     7381 tacagcaatc tcccaattga tggtctttat caatgaagcc tataaagtgg acgctctgcc
     7441 ttatgaatat atcgaaggat ttgttcaact actcgcaccg attgctcctc acatgggtga
     7501 agaactatgg gcaatccttg gaaatgatgg tggtatttct tacgcaccat ggccaacata
     7561 cgatgaagca gcacttgttg aagacgaagt agaagttgtt ttccaagtaa acggtaaagt
     7621 tcgtgcgaaa agcaatgttc cccgagactt aggaaaagat gagctagaaa aagcagcatt
     7681 agccaatgaa atagtgcaag aatatattga aggcaaaaca gtcagaaaag ttattgcggt
     7741 accaaacaag ttagtcaata tcgttgctaa ttaattggct ctttgtcaat aaggactgat
     7801 gagttgtgca aaatcaaatc tgggccagaa tgaacttcat tctgtctcag attttttgtt
     7861 atttcaaatt gattaattga ttgatcaaaa aggttttgat tttcatgttc tcaaacacgt
     7921 tcaatgtctt tttcttctga atggtcttgt aagagatctc tgtaatcatc aaatttttgt
     7981 ctattaatct tagtattttt tgatgtataa actacctgaa attgagagta cataccaaat
     8041 tcataaattt tttattagtg ggactagtga ttgtatggat tttgacgcat aatgaatgaa
     8101 gtatgagaaa aggagagaca aatggatcaa gaactattta ttatcgaagt attatcagct
     8161 aaacaaaaaa ttatcgagaa agcgaaaaaa agagcgaaaa gaataatgtt caagctttta
     8221 agcttagtat ttttctctgg tttgcttttt ttcggagctg tatcgttcct atatcgccat
     8281 atgagtgcag tagtccgttc agctgaaggg atgcaaaaat taaataattt gtatcacttt
     8341 tcgtggggag ttggcggaat aacaattgtt gtgcttattg tttttcttct acttatttgg
     8401 ctccgttacc atcaattgtc gcgtcatttc gatcaatggc ttagtcaaag agcacaacag
     8461 tttttacata ataaaccaac ttctcaagat gatgactatc tttattttga gaagacttcg
     8521 acgagtcaag cttttcggat acaaaaaagt gattgccagt tattgctgag atcacttgat
     8581 gatttaccta tctacttagg cgaaagcaaa tcaaaattcg gaatgaacac attccatatt
     8641 tttttactag aaaacgaacc cagatgggaa aaaagccagt cttcgcctgg tggatacctg
     8701 acaactaaga tgagtacatt agtcactggt tttttcctca ttcttttagc tggaacaatc
     8761 tattatggga gatacactca gccaaaaatg tcttctcata taaatgctgc ttctcacatg
     8821 acaatgaact caactaacga tacgcatata caaaagacaa atgaagggtt gttagctgat
     8881 cctaagcaaa aggtaaattt gacaacacaa aagataaatg atctcaaact tgatacagag
     8941 acgcactctc tctatatgac aacagatagt ggtgctaatt gggtatttgt gccgattgat
     9001 tcgcaatggt tgcgttttgg cgaatacacc ctgacaacgg ggctgattcc tgaaggttat
     9061 tggatggaca acacatatga tatttccgcc gatttctcat ggtttatcta ttcttctggg
     9121 gatgatgtac gtctgctgac ttcaagagat aatggtaaaa catggcaaac gtctattatc
     9181 acccaatcag ttcagccgat acgttatcga aaagcggtat tttatggcaa cgatggtcta
     9241 tttatctgta catcagccag tgggatgtct gcagaaactt tgtacattta cactactatg
     9301 gatggcggaa tcacttggat aaaaagtgga agtacaatga tcaatcagcc agtacaaaat
     9361 gtcagcttcg ttacaacttc tttaggtttt gtttcgacac gtgaaagttt gttttataca
     9421 aacaacggag gacgctcatt caaagaatca gttctgtcga ttccggaaaa ctatcagaca
     9481 ggggggatag atctttttca atcaccaaat gaagttactc aaataagcgc gaatacgtta
     9541 gagacaaaat tttatttgtt gaaaaaagga ccaatcgatg cagggaaaat gtttgcctgc
     9601 ctcttccgtt caactgataa cggggaaaca tggcaattcg ttcagcaact atcgcaagta
     9661 gaacaaggag attaaagaag agagattgtg agcctgacgt tcagcaccga gtcacaagaa
     9721 acaaattcaa aaaacagctt ctcatatttg ttgaatagtg taactcgtga ttcggagttg
     9781 acaagagcaa caagaagcga ttttgaagta tagcttctcg tattttacaa gatcgtgtct
     9841 tattgccgat aggttgtctc ttgaacaccg tttattcgta aaaagggatt gtgacaagtc
     9901 ttttgtcact acttaccgaa cgaaactgaa ggagctatcc aaaaagtatc attagtatta
     9961 ttctttgtgt ttctagtggc accactagta catttaaaaa agctatcgat catggactaa
    10021 tcatacatga tcgatagctt tttagataat gtttgttacg ctatctgaaa ttgactatta
    10081 taaagactgg cataaagcgt tggtgattgg agtagttcag aatgtgttcc tttttcgacg
    10141 atatcgccat tcttcattac aaggatcatg tctgcatttt caatcgttga caaacggtgt
    10201 gcaatcacaa aacttgtcct atcttttgtc acagcttcca ttgcttgctg aatcaatgct
    10261 tctgttcgtg tatctacgct agaagttgct tcatctaaaa taacgacagg aggattcgct
    10321 agaatgactc gagcaattgt caataattgt tgctgtcctt gagataacgt gccgttttcg
    10381 ctagaaatga tcgtatcata tccgtctggc agtgttcgaa taaagtgatc acattgtgcg
    10441 atttcagccg cttcgataat ttgttctctt gtggcatcca ttttcccgta ggcgatatta
    10501 tcagctaccg ttccttcaaa cagccaggta ttttgtaaaa ccatcccaaa agaacgacgt
    10561 agttctccac gtgaaagctt ggtaatatca agaccgtcga attcgatctt tcctttattc
    10621 agttcataaa aacgcatcag cagattgact aaagtcgttt ttcctgcacc agttggacca
    10681 acgatcgcta ccgtttgttt tggctttact gaaaaatcga cttgattcat caatatttta
    10741 tctgatgtgt aaccaaactg tacatttttg aaagctattt ttcctttcgg ttgttctaaa
    10801 agtcttgcat ctggaaggtc tggaatttca tctgtttcat ctaatatctc aaatatccta
    10861 tcgattgctg ccattgcgga ttggatcgag ttgatcacat aagaagcggt agaaattggt
    10921 tcagaaatct gattgatgta ctgaagataa gcttgtaaga agccgatcgt cacaccacct
    10981 gatagaacaa gcatagcacc gaataccgca cttatgatga aggcgagttg attaatgaaa
    11041 cgaatggccg gatagatagc acagttcata aattgagcac gtttaaaagc gttgtattgc
    11101 tttttattta cttcgtgtat agcagcttct gcatgagctt gctgattgaa tgttttgacc
    11161 actagattac cagctaagat ttcttccatt cgattattta aaacacccag ttcatactga
    11221 ttctgttcag aaatctgttt gtttttgttc gcaatcttag ttgtaacaat tgaactgcca
    11281 acgatcagca ataagacgag caaagtaagc ttgctttcga tataaaacag catcagacca
    11341 gcagcagcta ttgttacgac agaagtaaaa aattgattga tacctgtcaa taacacttga
    11401 gaaagttgat tgagaccggt agttgtccga ctaatgatgt cacctacttg atgattgtca
    11461 aaaaacgaca tcggtaatgt tttgaatttt tttgtgattt cttttcgcac cctcaatgtg
    11521 atcttttcgc ttaacgctgc cattgtccgt tcctgaataa atgaagtgat gctgcttaag
    11581 actgaacagc cgatcagcaa taagacaggg aacaacaatg cttgtttgat atcactgaaa
    11641 gaaagctgtc caatacctgt ttttcttatt tctgataaga gatcatcgat cccgattccc
    11701 atgatcaagg gcatggctac gactaatagg ttcccgaata aactgcatag aaacaatccg
    11761 taaaaatatt tatgttcagg tttgatcatt ttccaaaaac gtatcaaagc acgcgtgttc
    11821 aacgtttttt tcttttccat cgtatccctt ctttctatat tccttgtgat tttgcaaact
    11881 cctgataagt ttggcaatta gacagtagtt cttcatgagt accttgtcca acgatttttc
    11941 cttcatctaa aacaatgatg ttatctgctt gttggattgt actgaggcgt tgagcaacaa
    12001 tcacgagagt tttctccgac atgtgtttat gaagggctgc tcttaatgct gcatctgttt
    12061 tatagtctaa agcggagaaa ctatcatcga atacatagat ctctgctggt ttgatcaacg
    12121 ctcgagcaat acatatccgc tgtttctggc cccctgaata attagaaccg ccttgagcaa
    12181 caaagctgtc caatccgtca ggaagggtgt ccactagttc ttctaattga gcgatcttga
    12241 tcgctttaga caactcttct gttgaggcat attcatttcc catctgcaag ttgcttaaaa
    12301 ttgtcccgct gaataagaaa gccttctgag ggacataact gatgtgggag cggatctgtt
    12361 cttgagtcat ttgagtaaga ggaataccac caagggttac ttttcctatc gtagggtcac
    12421 taagtctcaa gagaagtttc gccaaactac tttttccaga tccggtcccg ccaacgatcg
    12481 ctgttgtttt tcctttaggt attgtgaaat caatatcttc tagaactgga tcagagattt
    12541 cagtatattt aaatgtgaca tgttcaaaag cagctgcatt tgtctgatca aaggttggtt
    12601 cagctactgt tttgatcgtc tcttcctcaa tttcgatctc tgcttgcagt atttcttcta
    12661 tccgtcgtaa tgaggcaaac gatttaggga taatgaccaa taccatagct gcgatcatca
    12721 agtaagcaag tgtcagcata gagtattcga ctacagcagt cacgacaccg atttgtaatg
    12781 ctccttctgc tacaagattt ccgccaaacc acaaaataga agcataaacg attcccatga
    12841 gcaaaaaagc aattggagtg acccaagcaa atattcggtt cactttgatc atttgattag
    12901 catagtcagc aaagctctta ttcgttcgct tttcttcata tgattgatta tcaaaggcac
    12961 ggatcatatt aatcccagta aagaattctc tcaatgtgat ggtgatccga tccatttttg
    13021 gctggatact caaagataaa ggtgcccctc gtttcatcag tcgataaaca atccatacat
    13081 aaaaaacaac agagatcaat gtaacccatg ttagtaatag agaataacga aacatcatga
    13141 gaatggtgaa aacgcaaata accggtgctg gtaagatcat ttggcaaaat aaaacaatca
    13201 tttgctggac attatcgaca tcattcgtca ttcttgtcaa aagagatcca gaactaaaac
    13261 gactgatatc cttcattgac agctgttgga atttacggaa atatttctcc cgcgtttgga
    13321 atccaaacct agcggaagcc aatgctgaaa agtaactaga ggcaactgca gcgattgttc
    13381 ctagaaacgc tacaagcagc atttcaactc caattttttt tataactgag gcttcgccac
    13441 ttgctatacc tgtgtcgatc agctttgcta ctaattttgg tacacctaat gtaccggcga
    13501 tttctaaaca gatcatcaca aaggtaagga gagcgagtcc tttgtttttc tgtaagaatg
    13561 tttttaagat agacatatta ttcctccttt tttcctgata cttttcgatt ctataccttc
    13621 aagtaacttg aaggtcaata gaaaaaagaa taaactgaaa aagaggtgag aaaaaatgaa
    13681 aaaaatgtat acgattggcc aagtatcgga actatttgat ttgccaaaat caacgatccg
    13741 ctattgggat gaacaaggat taatccgttc aagtagacaa gaagagaatg attatcgatt
    13801 gtttgatatc gatgatattt ttatgcttta tgatatcgtt ttttatagaa aattagatat
    13861 accgatcaaa cagatgaaga atttatatgg aaaaacattg acggagttat atgagacact
    13921 tgatgaaaca gaaagaagaa tccataaaga actggtggtg atgaaacaaa aacaaaaaga
    13981 gataagggag caaaaaaaac agttgaaatt gatgatcgat acaaacgagg aagaatttcc
    14041 tgtagaagag atcccttttg actgtatgat ctcaacggag tttgaagata ttgtggaaat
    14101 caaaaaattt ctacctaact attcaagctt tgggatgatg agtatgccag cttccagttc
    14161 acagacgatg tatggatttt ttatcgaccc tagtgaagta catttattta ctcaagacgt
    14221 gatttgggag aaaaaagata cggctgttta tcgtcgattc ttattgaaat ccgagatgaa
    14281 ccatgcggaa cgtaataata ttttagaaat cagagaaaga atgtatgaaa aaggatggaa
    14341 aacaggagaa gtgatcggcc agtatctatt gacgaatacg gatgaaaaca atatcagaac
    14401 tgaatattat catgcatgga tagaaatgaa aaaataggaa atatgtgttt aagataactc
    14461 gaaaatgatg gttgttttag ttataaagaa aaattttctg gttcgattac gggtttaatg
    14521 tgggggatta ttagtcgtgg atagatagaa ttttagtttt ttagaggaaa tatgtcttta
    14581 aaaatggaat ggttgcaatt cttagcgata tatacgatgt aattgtcctc ttaaaaaagt
    14641 atggaaaaat aattgtattg ggtgttatgt ataattcatg gtgaactgag actaatgaga
    14701 agaaaaattc atattgttat gagtgagatt gtatcagatg agaatgataa attaaatagg
    14761 tgcataaaag cattacactt aaaatacatt gttgcttaca ttattacaat atgttaataa
    14821 ggcaaaaata aatagatgga ttgaataaac atatttttta atcaaatagt ccctactgtg
    14881 actttcctca gctatcggtg atactaccga tagcattttt ttgcttataa aataattata
    14941 agtatttaga gatttttctt ctaacattct tcaaacaaga aaaatcacaa aggttattgt
    15001 tatttttata tattcttttt aagaatgatt gtttttttat agaaatttta aaaaataaaa
    15061 cacgttattg atggatagtt agtttgttat ttggtaatat tatagaagtt gactggtagt
    15121 gatttatttt tcgtcgtttt ataaaaatgt tattttttgg aaaaattcac aaatatgtaa
    15181 cgaatgtttt tctggaattt gctgtgttaa aaagcagtat gactaatata aatactattg
    15241 tcatttatgc aattttttat ggatagtaat tttagagggg aatcaatgaa ggaagaatag
    15301 aagggaaaaa tagttgcaaa tacgccagtt agcaaaaata gcaatacatc ttcaagcttt
    15361 aaaattcgag agaatcatct tggtagtgga atcaactaca acaaaaggat taaaccaatt
    15421 aatagataga attaccaatg gaaaaataga aaaggtagtt attctataca aaagttgttt
    15481 aattcgcttc ggttatgaac ttagtgaaac cctttgcaat atatggtaca gaatatagaa
    15541 attattgaca ttacagaaaa tgatgaagat ttaatacaga ttgtcactat ttgtagatac
    15601 cttttacaag gaaaccgagc caataaagct aagaagatga ttaaggagtt gatggaagac
    15661 gatacaagtt aagaaagtga aactacgccc aaataaagag caaaaagagt aattggacgt
    15721 ggaaaatgca agaaatgaac catcgattcg gtggacagtt tattagtaat gacggtttga
    15781 gaacatccat gacgagaatg aaggaacatc ctaaatgcgt ttagttgaac caagtgccaa
    15841 ataatatggc caaataagcg atcagttatc gatgaatccc aagtattcta aactatatat
    15901 tcattttgat gctaagcatt gatatatttt tttgggattg atgaaggtta accaatacag
    15961 ggactcactg atgaaattct agagtctgac cttggtatca acgatttagt gattgtttct
    16021 aatggaaaag tataaaagta tataagtaca ttaacaaaat aactattagg gttacgctcc
    16081 agcttgtgca aggcactggc aggaggaatc gtgaaatcat aattatggtt aagccacacg
    16141 gacaactaaa agtcatacaa agctatcgaa tcaatcaaca gaagacaatc ggcaatcatt
    16201 agctttgttt acagcttaca ttgcaacgct tgagttgaac cgaaaatacg agggaggtaa
    16261 cgcagccatt ttgagttggg gaatcttttt agaaaattat cgggtacctt gttgtttagt
    16321 caaaattata agggggagta atagtgagga acaaatttca taaactgtta agtatgtttc
    16381 tagttatgct tattgtgatc aatcaatttg ggaacataac ggttctagct gagtctttag
    16441 cagaacagcc agagcgttcg ctgacgatcg aagttgatca agaggaggct gctttgggtg
    16501 agcagttcca gttgcgagtc agaggaacac cagacctgct aaatgcattg gtcattgata
    16561 agagtgagga tttgattgaa ggggaagtga agcaactcaa tgattacgaa cgtctgatta
    16621 cttattcttc ccagcattca ggtaaatacg aattgaccgc aagaagcggt gatattcaat
    16681 caaatacggt ctcaatagaa atcagtgtac cagaagaaag cacaagtgag tcttttaatg
    16741 taccagaaga aagcacaagt gaatctttta atataccaga agaaagctta agtgaatctt
    16801 ttaatgaagc cagatcatct gagccatcta taacgcagga atctcagatt gaaagacaag
    16861 aggagctctt gaattatgac atcggcatct ctgctgagcg ccccactttg ccgagcaggc
    16921 atacacaaac gttaaaggga tacattcaag gaggcgctgg cgatattctt gaagaatatg
    16981 gggagatggt cgtacgagtt cctagagcat cgattggctt aattgagttc attagagtgg
    17041 aggtcgctga tgattcacct ttaaagccac tggcatttta ccaagaaggc acagacgttg
    17101 tttttccatt tacctttgat tatgaaggat taaatgaagc cgctcagtcg tatacattca
    17161 gtatcacgtt tgaagttgct ttctttcaat caggcggtac tgagccaaat aatgactttc
    17221 aagtgagtct tacttatgct gagcgagcag caaacacgga attttcaatt acgcctattc
    17281 cttcatcaag acccattttt tcgaaattat actatggctc tagagaaatc gacagccaag
    17341 gaaatcttgg gaatctgctg attgacgggc aagacgtgac caacagtaat aaatttattt
    17401 tgcaggtaaa ttatgaagga tcacgaaaag acggtgtagt ggttaccgat gtactaccgc
    17461 aaggaacaac tttggcaccg aatccgacta catggagttc cactggaaat agagaaccta
    17521 tttcaggtgg ggttcggatc gcagaagtaa cgaatccagg tgaagcaaac gttagaactc
    17581 attatgtttc cgaggaattt gtggacaaag tatcttttaa taaggaaacg aatacacttg
    17641 ttgtggactt tggtaatcta gcagagggtc aatcgtattt tattgaatat gctcttgaag
    17701 tgaccgatgt cgacttgttc aatgaaggaa gacaagagga tgggttttct cggaatcgag
    17761 cgagtatgac ctacaataat caggtaatga gccgagacta ccgcttgaga atccctgaaa
    17821 gtaatcttcc ggcaatctct ttaagaaaaa gtgtggacaa aaatgttatc aatctcaatg
    17881 aaaatgaact aacgtatact ctgcatgtct cgatgttttc tggaacaaga aatacgcttg
    17941 ttattgaaga tgttttggaa gaaggcatgg aatttgtgtc atttctatca gatattccct
    18001 ctttcattga tgccgaagtc aataatggaa tagttcgttt cagtgcgaat aatacgattg
    18061 gtggtccgac aagttttacc ctctctttta aggtcaatgt agaggcgtat gcggtcggcg
    18121 atcaaatttt aaactttgca acgcttgtga atagtgacaa tcgattgaat acgagtactg
    18181 taagaacaag gaaaatcgat gggcgtatta aagtaattaa aactgatgag aatggccaaa
    18241 ggctggcagg agcaaaattt gaaattttat caagcgattc acaagtgata caagaaggta
    18301 taacggcaga aaacggagaa tttttaagtg aaccgcttcc tgtaggtagc tatcaagtcc
    18361 gagaaacaga agcaccggaa gggtatcttt tagatgcgac ggtacatgat gtttttataa
    18421 atgaatactc catagaagaa gtgacgttaa ccattgaaaa catccttgat acgggtggtg
    18481 ttgaactaat taaacatgct gaaggcagtg gtgaagtcct tcaaggagct gtttttgagt
    18541 tacagaaccg agaaggagaa acgctacaaa ccggtttaac taca
//
LOCUS       DASHHB010000055        18189 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.057, whole genome shotgun sequence.
ACCESSION   DASHHB010000055 DASHHB010000000
VERSION     DASHHB010000055.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18189)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18189)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18189
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.057"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..506)
                     /locus_tag="U2K71_002163"
     CDS             complement(<1..506)
                     /locus_tag="U2K71_002163"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM7316195.1"
                     /translation="MDNHTRKLLGLTDKHLFFDEEWLIEKEYKGVQAQFIKGKLDDSS
                     SYCEHCRSVRIIRNGSYTTRTQMLKVKEKLTILELKRTRFLCHDCGRTFSAKTDLVDE
                     HHQLTKELKQVILMELYENQSRKLIAKKYFVSDGTVTRILREATKHYQPRMNFLPTVL
                     CMDEFKSM"
     gene            779..3250
                     /locus_tag="U2K71_002164"
     CDS             779..3250
                     /locus_tag="U2K71_002164"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HEM7316196.1"
                     /translation="MKNKWINRSKKKKVTQRSSDKVIRLFPSYKEGLTADQVKERIEK
                     GAANNSVDPTFKTNQQIVLENIFTYFNLIFLILAILLCLVESYKNLTFLPVIIANTGI
                     AIFQEIRSKKILDELNVLNTAKVNVIRDGIEQTIDIEDLVIDDIVILETGYQIPADAE
                     VVEGKLQVNEALLTGEADEITKEIGDPLMSGSFIVSGKAYARLDKVGADSYISQLTVK
                     AKTMGEGEQSEMIASLNQLIKWIGIIIIPIGILLFSQSYFFNGNTIKESVVAMEAALI
                     GMIPEGLYLLTTIALALSATKLAKQRVLLHNMKSIETLARVNVLCVDKTGTITENKMS
                     VQKMIVSKKQEIISQSDQLEACIADYAKAMAGDNATMEAVKAYFTKTTDQSYHHVIPF
                     SSVQKFSSVALADKVYVLGAPEMVLRDQLPEYAEEFVSFAEKGYRVLVFGVYDGILEE
                     PVLTEAVTPLGYILIANPIRKEARATFDYFKKQQVAIKVISGDNPLTVSNVAVQAGIS
                     HADQYVDASKLSEEEYEAAVEKYTVFGRVKPEQKKIFVQLIKRNNTVAMTGDGVNDIL
                     AMKEADCSIAMASGNEAAMQASQVVLLDSDFSRMPEVVAEGRRVVNNIERSASLFLVK
                     NIFSFLLSLFSVIFALTYPLEPSQITLISLFTIGLPSFLLALEENKKRIRGKFIMNVM
                     EKAVPGGLTDMIVVGALVICGVTLNLNKTDVSTASTMLLIAVGFLVLYKICSPLNKFR
                     SQIILFCASGNFFSVIFLHKLFSITGISAVSLLLLSILFFSADSIFRHLTTLVEYLFE
                     RKNEKEQKPFSRVAKKFLSIFKREN"
     gene            complement(3353..5089)
                     /locus_tag="U2K71_002165"
     CDS             complement(3353..5089)
                     /locus_tag="U2K71_002165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component sensor histidine kinase"
                     /protein_id="HEM7316197.1"
                     /translation="MKWKKIYERLFFLILLVSLFFGGWQLISHYFQQQIVEQQESYLT
                     KKAQLLIRQLDVENLQAAQNKTALEEFVHQSNERITLMDATGKILFDTNNETLNEQRN
                     SRPEIKAVLNGGNLGSALRKSTTLNEDLLYVALPVKKSGQLEAILRIAEPTSGFLPRT
                     ESFRRWVFFFFLSFFLVLAGMIYYLIYQKNQPLKTVLPVLKKMVQNPNQAEVIMQTPD
                     QWEELYQTINALSEQMSKMYRACTTTEEQLYTLLNELMIGVFLIDDADSRLLLLNPKM
                     QLHLNVVSYQPEQRYTEVIQEPKLIQLIHQVTPEHPLIHQEITLTEGRQTLDLSLRYF
                     TNSEGTGQILGVAYDLTKVRRLEKLQKDFVGNVSHELKTPVTSLIGFTETLLDGAKDD
                     PQTLTSFLEIMQKDAIRLDKLIREIIQLSKDGENSYEIQTIYTEPYFQQIVQNYQPII
                     EKKRLTIRLIGKNEPFTTKTDILYPIIKNLIENAVQYSKSDSEIIIRYQATDDLSFSV
                     QDFGIGIDIEDQERIFERFYRVDKARSRHSGGTGLGLAIVKDYVQLLNGTITVDSHLG
                     TGSTFTVTIPKM"
     gene            complement(5086..5790)
                     /locus_tag="U2K71_002166"
     CDS             complement(5086..5790)
                     /locus_tag="U2K71_002166"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7316198.1"
                     /translation="MKKILVVDDEPSIVTLLTFNLEKEGYKVTSATDGGEGLELALEQ
                     SFDFIILDVMLPTMDGMEITQRLRQEKNETPILMLTAKDDQVDRIIGLEIGADDYLTK
                     PFSPREVLARMKAIFRRIEPRKQSKDEAEPEYLSIGQITADLTNYQVTVDDHLIELTP
                     KEFELLVYFMKRKDRVIDRDTLLDRIWNFDFAGQSRIVDVHVSHLREKIERDPKHPKY
                     LVTIRGFGYKFQEPKR"
     gene            5921..6424
                     /locus_tag="U2K71_002167"
     CDS             5921..6424
                     /locus_tag="U2K71_002167"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF523 domain-containing protein"
                     /protein_id="HEM7316199.1"
                     /translation="MIGISACLGGLACRYDGQKKEVPELMELVETGEAVMVCPEVIGG
                     LPIPRDPAEIIGGNGFDVWKDQAKVWTTSGEDVTEAYKNGAIKAYQKLQEKHIDLVIM
                     KEKSPSCGTHSIYDGSFSGIKKNGAGVATAYFIQQKMTVLSDEEWLKTRGEQNGNRKD
                     QSNERAL"
     gene            6384..6722
                     /locus_tag="U2K71_002168"
     CDS             6384..6722
                     /locus_tag="U2K71_002168"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative DNA-binding protein"
                     /protein_id="HEM7316200.1"
                     /translation="MEIEKTNRMNALFEFYSTLLTEKQMNYMELYYADDFSLGEIAEE
                     YEVSRQAVYDNIKRTSKILEDYEKKLHLFSNYIVREQVLENMTAYITDKYPEDKKLLE
                     YVQQIQAIEE"
     gene            6743..8161
                     /gene="ffh"
                     /locus_tag="U2K71_002169"
     CDS             6743..8161
                     /gene="ffh"
                     /locus_tag="U2K71_002169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HEM7316201.1"
                     /translation="MAFESLTERLQQAMGKIRKKGKVSEADVKEMMREIRLALLEADV
                     NLQVVKDFTKRVRERAVGAEVLESLSPAQQIVKIVDEELTITLGSETAELNKSPKIPT
                     VIMMAGLQGAGKTTFTGKLANYLKKNENARPLLIAGDVYRPAAIDQLKVLGQQLDVPV
                     FDMGTEVSPVEIVRQGMELAKEKKNDYVLIDTAGRLHIDETLMDELKQIKELTQPNEI
                     LLVVDAMTGQDAVNVADSFNQQLGITGVVITKLDGDTRGGAALSIRSVTGAPIKFIGS
                     GEKLTDLEVFHPDRMASRILGMGDMLTLIEKAQQDYDEKKAEELAQKMRENSFDFNDF
                     IEQLDQVMGMGPLEDLIKMIPGMNQVPGIENVKVDPKDVERKKAMVYSMTPAERANPD
                     LLNPSRRRRIAAGSGNSVVEVNRMIKQFKESRKMMQQMSKGDMNIPGMDQMFGSGVKG
                     KLGKMAMNRMIKKNKKKKKKRK"
     gene            8273..8851
                     /locus_tag="U2K71_002170"
     CDS             8273..8851
                     /locus_tag="U2K71_002170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HEM7316202.1"
                     /translation="MRCPWSEQTESMRQYHDTVWGVPEYDDQKLFRKLMLDINQAGLS
                     WQTILNKSEAFDEAYDRFDIKKVANYGETKISELMENKGIIRNRRKIEAAVQNAKAVL
                     KIQEEIGSFSEYLWSFSNGKIVDSSYHSQEEVPTTSPLSDELAKDLKKRGFKFIGSTT
                     IYAFLEAVGIINDHLDSCFRKEEVVRIGRKGE"
     gene            8855..9376
                     /locus_tag="U2K71_002171"
     CDS             8855..9376
                     /locus_tag="U2K71_002171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HEM7316203.1"
                     /translation="MRKIIFYGAISLDGYLATKTDDLQWLFDTPTGKQTTYEAFYETI
                     DTTIMGRKTYQEAKKYLETEAIYPEKKNFVFSTHAQLQLPDAEVVNEDPVAFLKNLQM
                     TEGKDIWVVGGGRLIKPLLEHQMIDEWYIQITPVLLGDGIPLFQKGTYEARFEMLDTT
                     RFGEFIELHYVRK"
     gene            complement(9455..10009)
                     /locus_tag="U2K71_002172"
     CDS             complement(9455..10009)
                     /locus_tag="U2K71_002172"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM7316204.1"
                     /translation="MTKKIGFFIGSLRKDSYNKKVAEAFAKLLPEGYESVFVKIDDLP
                     FYNEDLETPEQAPAEWTRFREEVRGLDGVVFVTPEYNRSVPAVLKNALDVGSRPYGHS
                     VWDHKPGLVVTASPCGIGGFGANHHLRQSLVFLNVPTLQQPEAYIGNVANLIDENGNI
                     VEGTLSFFQTILDAYLDFSNKLTE"
     gene            10262..10537
                     /gene="rpsP"
                     /locus_tag="U2K71_002173"
     CDS             10262..10537
                     /gene="rpsP"
                     /locus_tag="U2K71_002173"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016175618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HEM7316205.1"
                     /translation="MAVKIRLKRMGSKKSPFYRIVVADSRSPRDGRFIETVGTYNPLK
                     DPAEVVLKEDLVLDWLSKGAQPSDTVRNILSKEGVMQKHHEAKFSKK"
     gene            10549..10800
                     /locus_tag="U2K71_002174"
     CDS             10549..10800
                     /locus_tag="U2K71_002174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HEM7316206.1"
                     /translation="MKDLSDLVLTIVRPLVTYPDQVKLDVVESKDFYEYNLTVAPEDI
                     GRIIGKQGRVAKAIRTIVYGVRINAPKKVRLNIIDDKEK"
     gene            10925..11716
                     /locus_tag="U2K71_002175"
     CDS             10925..11716
                     /locus_tag="U2K71_002175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316207.1"
                     /translation="MRKKMLFIPFALLLSACSSVTQESDNTHLSSSISETVTSPEKSS
                     TKTASTTEQTMATSNNEKKTALDQLKEQQPNVPMPLDVPVSSGYLNIAATHTKQGYSI
                     LYYRTDRPLGLNADELNQETPIATYLYQYGFASSQETIQVLQPFEIDTNGQQVDLGSR
                     ITGYQQGAAGSSFLEWQEGNWRIRIRGNNIEGQDPLLLAKEIVAYLEENSLPAPEQFG
                     KITVDMGDTTNRAVEVSWQEPKNAYTITHQDPMSALKMAVSMKRL"
     gene            11886..12407
                     /gene="rimM"
                     /locus_tag="U2K71_002176"
     CDS             11886..12407
                     /gene="rimM"
                     /locus_tag="U2K71_002176"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HEM7316208.1"
                     /translation="MTEYLNVGKIVNTQGIKGEVRVISTTDFPEERYKKGTVLTLFQD
                     GKAPVELTVKSHRKHKNFDLLSFEGHPSINDVEKYRDGILKVSKDNSVDLAENEFYYH
                     EIIGLHVLEDGTELGKVKEILSPGANDVWVVQRPKKADILLPYIASVVKEVDLEAGVV
                     RVEIPEGLIDDED"
     gene            12397..13158
                     /gene="trmD"
                     /locus_tag="U2K71_002177"
     CDS             12397..13158
                     /gene="trmD"
                     /locus_tag="U2K71_002177"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HEM7316209.1"
                     /translation="MKIDVLTLFPRMFEGPMGESIIGKAVDKGLLDINISNFRDYSDN
                     KHQTVDDYPYGGGAGMLLKVQPIYDNIQAIEKAAPDVKKRVILLDPAGKRFDQKMAEE
                     FSQEEHLVFICGHYEGYDERIRTLVTDEVSLGDYVLTGGELGAMVMIDATVRLLPDVL
                     GNQTSAQTDSYSTGLLEHPQYTRPAEYKGMKVPEVLTNGNHKLIEEWQLKESLRRTYQ
                     RRPDLLETLEWTPQMTKFLEEIKKEEQEKTADEAK"
     gene            13294..13641
                     /gene="rplS"
                     /locus_tag="U2K71_002178"
     CDS             13294..13641
                     /gene="rplS"
                     /locus_tag="U2K71_002178"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HEM7316210.1"
                     /translation="MNPLIEELTKEQLRSDIPAFRPGDTVRVHAKVVEGTRERIQLFE
                     GVVIKRRGAGISETYTVRKVSNGVGVERTFPLHTPRVAKIEVVRYGKVRRAKLYYLRA
                     LHGKAARIKEIRR"
     gene            complement(13773..14909)
                     /locus_tag="U2K71_002179"
     CDS             complement(13773..14909)
                     /locus_tag="U2K71_002179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM7316211.1"
                     /translation="MWIETKTDKNGKKVYKYNERYIDPKTRKRKKVSITYKNKSRETQ
                     KVALLELNKKIDIKLNEKTLQKPNLTFHELVEEWLVIYKRQVKESTYYPTNNILNTIK
                     KKMPETYIVSGINTVDLNNIFEDMIYKDDLSNKYVSVIKSKLNLLFSYAMKKGYVEKN
                     PINEVVIDYKRESKTIKIKDKFLEDDEYKRLVDFTTSHNKRYSLLFQWLYLTGMRPGE
                     AIALSKDDVHITNNNASVVINGTMMYRERSIADMKKSDSTKTAAGMREIDLPKKAIAI
                     YNELLELNPKGQFLFQTTKGTPFQLTAINTYLRNHKADMKIDKKLSSHIFRHTHISKL
                     AELGTPLYAIQDRVGHENSDITEKIYLHVTKGVKEKLKEDIEKL"
     gene            complement(15026..15718)
                     /locus_tag="U2K71_002180"
     CDS             complement(15026..15718)
                     /locus_tag="U2K71_002180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316212.1"
                     /translation="MKKVILGLVFGAFFLTGCSQSNVVDNTGKTNEKDSSVTLSESTT
                     QSSKNSQEVDGPLLKVGQWTKEKRDTSGKIELIGTASPQSDIPLGDIGIYISNIKLLK
                     YSNYADPSAAGYYDANNHKDSEGNFYGLQIAFKVKNNSEVDYGYNGLAHAILDNGQQI
                     DFSSDDLMHTMTTNSFFKKTESKEFYSLAYLDPSKVNDITKVTIKTGVLYNPSDYSTV
                     AESAESTFNINR"
     gene            complement(15856..16509)
                     /locus_tag="U2K71_002181"
     CDS             complement(15856..16509)
                     /locus_tag="U2K71_002181"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316213.1"
                     /translation="MKKVSVMLLLSTALLLSACSNNKKAESTDATSNQETKVSKTKET
                     TETSSSTSKSTSKTDSSSTVTSSNQATAEPSPTVISSSQDTIQTAPQEETYEQMKQRT
                     LQSTPADRANWSNKEWEAFGVALYENGLTTDDAGNIISQDQKEQQAASQQNPEDQQTS
                     GQQDADTLSLTDFVNKYGMSPVAWKVQNGMSEEEALRTTQQKTSGEVQLGFSKYGIQ"
     gene            complement(16559..16987)
                     /locus_tag="U2K71_002182"
     CDS             complement(16559..16987)
                     /locus_tag="U2K71_002182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ImmA/IrrE family metallo-endopeptidase"
                     /protein_id="HEM7316214.1"
                     /translation="MEMDTINLVEELKRKYRSANPFYICEKMGIKIQYVPFIENPKGQ
                     FQEIRGRAIIFLNDELRDSEERFYICAHELGHAIFHRGLSSYYVSTRTSRSKSESEAN
                     CFAANLIVSLYKEDNDQYPKKIDELKNLYGLPESAYRFLI"
     gene            complement(16992..17366)
                     /locus_tag="U2K71_002183"
     CDS             complement(16992..17366)
                     /locus_tag="U2K71_002183"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7316215.1"
                     /translation="MNLLDRIKELAHKRGISITQLEEELNIPKNTIYQWKNRTPSTKR
                     LQLVADYFNVTTDYLLGRNQVPDWATKDEVVELDKLLDSNVNMSYGGETLTPEQVQRV
                     KDILIGTFWDIVKEDKEKGKKM"
     gene            17651..17836
                     /locus_tag="U2K71_002184"
     CDS             17651..17836
                     /locus_tag="U2K71_002184"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7316216.1"
                     /translation="MSENLDLKIRAEMRKRRMTFKELAALVGISGAYLSDILNGNRDG
                     KKAQQHIETVKKILDIR"
     gene            17849..18097
                     /locus_tag="U2K71_002185"
     CDS             17849..18097
                     /locus_tag="U2K71_002185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316217.1"
                     /translation="MTKLKKQDFVKKYNYSPSTYQRRMSELKNTAIFSAAYERVTGQE
                     VWINTELYDKFLSFKSYNRLRTRKITPKEFIEKHLVDL"
BASE COUNT         5815 a         3268 c         3575 g         5531 t
ORIGIN      
        1 ttcattgatt tgaactcatc catacacaac accgtcggaa ggaagttcat acgtggctgg
       61 taatgcttcg ttgcttcacg caagatccgt gtcaccgtcc catccgagac gaagtacttc
      121 ttagcgatta gtttacgtga ttgattttcg tacagttcca tgagaatcac ctgcttcagt
      181 tcctttgtta gttgatgatg ttcatcaact aagtccgttt tcgcagaaaa ggtccttcca
      241 caatcatggc agagaaaacg ggtacgtttt aattctaaaa tagttaactt ttctttgact
      301 ttcaacatct gagtgcgtgt cgtataggaa ccgttgcgga taatacgaac cgatcgacag
      361 tgttcacaat aggaagaaga gtcatccaat tttcctttaa taaactgcgc ttgaacgcct
      421 ttatattctt tttcaatcag ccattcttca tcaaaaaata aatgtttatc tgttaatccg
      481 agtaattttc tagtatgatt atccattgag agactccttt ggtaggatga cgttttggtc
      541 gactaaattt taccaaaagt gagtctcttt ttgagttgaa acgaagaaaa agtgttagta
      601 gaaaatcgcc ttcgattttc caccaacaca aaatattata gaaccctttt tttttgctat
      661 atgacaggaa tgaaaggtgt ttgcaagctt aatgtgagga acactttcca tatctgttct
      721 atactggata ggagaagttt ggaaatatcc agaaaacgaa atcaatcgag gtagccacat
      781 gaagaataaa tggataaatc gaagtaaaaa aaagaaagta acacaaagat catcagataa
      841 agtcatccgt ctttttccat cgtataaaga aggattaacg gcagatcaag tcaaggaacg
      901 aatagaaaaa ggtgcagcaa ataattcagt cgatcccaca ttcaaaacaa atcagcaaat
      961 cgtattggaa aacatattta cgtatttcaa tttgatcttt cttatcttag ccattttgct
     1021 ttgtttagta gaatcctata agaacttgac ttttttacca gttatcatag ccaatacagg
     1081 aatcgccatt tttcaggaaa ttcgatccaa aaagatcttg gacgaactaa atgttttaaa
     1141 tacagcaaaa gtcaatgtca tacgagacgg gatagaacaa acaatagata tagaagacct
     1201 tgtaatagat gacattgtta ttttagagac tggatatcaa attccagcag atgcagaggt
     1261 cgtggaaggg aaactgcaag ttaatgaggc tttattgaca ggcgaagcag atgaaataac
     1321 caaagaaata ggtgatccat taatgtccgg cagtttcatt gtttccggaa aagcttacgc
     1381 ccgattagac aaagttggag cagattcgta tatttcccag ctgacagtca aagccaagac
     1441 aatgggagaa ggagaacagt cggagatgat tgcttctctt aatcaattga tcaaatggat
     1501 tgggatcatc attattccta tcggtatcct gcttttttct caaagttact tttttaatgg
     1561 aaacacgatc aaagaaagtg tcgttgcaat ggaagcagca ttgatcggta tgatacctga
     1621 aggactgtac ttgctgacga cgattgcatt agcattaagt gcgactaaac tagcaaagca
     1681 aagagttctt ttacacaata tgaaaagcat tgagacattg gcaagggtaa atgtcctttg
     1741 tgtagataaa acaggaacga tcacagaaaa taaaatgtct gttcaaaaaa tgatcgtctc
     1801 taaaaaacaa gaaataatca gtcagtcaga tcaattggaa gcatgcattg ctgactatgc
     1861 aaaagcgatg gcaggagaca atgcaacaat ggaagctgtt aaagcatatt ttacaaaaac
     1921 cactgatcaa tcctatcacc atgttattcc tttttcttct gtccaaaaat tcagcagtgt
     1981 agcgttggca gacaaggtgt atgttttagg agcgcccgaa atggttttgc gcgatcaatt
     2041 gccggaatat gcggaagagt ttgtttcttt cgctgaaaaa ggctatcgcg tgttggtctt
     2101 tggtgtgtac gatgggattc ttgaagaacc agtattgaca gaagcagtca caccattagg
     2161 ctatattctg attgctaatc ctatcagaaa agaagcaaga gccacatttg attacttcaa
     2221 aaaacagcaa gtagccatca aagtgatttc tggagataat ccgctcaccg tatccaatgt
     2281 agctgtccaa gcagggattt cacatgctga tcagtacgtt gatgcatcta aattatcaga
     2341 agaagaatat gaagcagctg tcgaaaaata tacagttttt ggacgcgtca agccagaaca
     2401 aaagaaaatc ttcgttcaat tgataaagcg taacaataca gtagctatga caggtgatgg
     2461 cgtgaatgat attctagcca tgaaagaagc agactgcagt atagcgatgg cttccggaaa
     2521 tgaagccgcc atgcaggcct cacaagtggt cttacttgat tctgactttt cacgcatgcc
     2581 agaagttgtt gcagaaggac ggcgcgttgt gaacaatatt gagcgatcag ccagcttgtt
     2641 tttagtgaag aatattttct cctttttgct ctcactgttt tcagtgatct ttgctctgac
     2701 ctatccttta gaaccatcac aaatcacatt gattagtttg tttactatcg gacttccatc
     2761 tttcttgctt gctctagaag aaaacaaaaa gcgaatcaga gggaaattca tcatgaatgt
     2821 gatggaaaaa gctgtgccag gtggactaac agatatgatc gtagtcggtg cgttggtgat
     2881 ctgcggggtg actttgaatc taaataaaac agatgtttct acagcatcta ccatgctttt
     2941 gattgcagta gggttcctcg ttctctataa aatctgttca ccgctgaata aattcagaag
     3001 tcagatcatt cttttctgtg ctagcggtaa ctttttttcg gtcatctttt tgcataagct
     3061 gttttcaatc accggtatct cagctgtatc acttttgctg ttgagcattc tatttttctc
     3121 ggcagattcg atttttaggc atttgaccac attggtagaa tatttgtttg aacgtaaaaa
     3181 tgaaaaagag caaaagccgt ttagtcgcgt agctaaaaaa tttctatcga tatttaaacg
     3241 agaaaattga cgaaaagtat ctcaaaaaag agtaaaaaag aacaaccact cgtgtatctt
     3301 tcgaagcagg atgagtcatc caactttaaa acgcacgagc agttgctctt ttttacattt
     3361 tcggaatggt aacggtgaaa gtcgaacctg ttccaagatg actgtctaca gtaatcgtac
     3421 cattaaggag ttgcacgtag tcttttacaa tcgctaaccc taatccggtc ccgcctgaat
     3481 gtcggcttcg cgccttgtct acgcggtaaa aacgctcaaa gatccgttcc tgatcctcaa
     3541 tgtcgatacc tataccaaag tcttggacag aaaacgatag atcatctgtt gcctgatagc
     3601 ggataataat ctcgctgtcg ctttttgaat attggacagc attttcaatt agatttttga
     3661 taattggata aaggatatct gttttagtag taaatggttc gttctttcca atcaatcgaa
     3721 tcgtcaaccg tttcttttcg atgattggct gataattttg aacgatttgc tggaaataag
     3781 gttctgtata aattgtctgg atctcgtaag aattctcgcc atcttttgac agctggatga
     3841 tttcccgaat cagtttatct aaccggatcg catctttttg catgatctca agaaaagaag
     3901 tcagagtttg cgggtcatct tttgcaccat ccagtaaagt ctcggtaaaa ccaatcagtg
     3961 aagtcacagg agtttttaat tcgtgagaga cgttgccaac gaaatccttt tgtagctttt
     4021 ctaaacgtcg cactttcgtt aagtcatacg ccacccctag aatctgtccg gtaccttctg
     4081 aattagtaaa gtagcgcaaa ctcaagtcta atgtctgccg accttctgta agggtgatct
     4141 cctgatggat cagcggatgc tcaggagtca cttgatgaat cagctgaatg agttttggct
     4201 cttgaatgac ttctgtgtac cgctgttcag gttgatagct taccacgttt aaatgtagct
     4261 gcatctttgg attaagcagt agtaaacgag aatcagcatc atcaatcagg aatacgccaa
     4321 tcatcagttc gttcagtaac gtatataatt gctcttctgt ggtagtacac gcccgataca
     4381 tcttactcat ttgttcgctc aacgcattaa tcgtctgata caattcttcc cattgatcgg
     4441 gtgtttgcat gattacttcc gcctgattag gattttgcac catttttttt agtacgggca
     4501 gcacggtttt caatggctgg tttttttgat agatcagata atagatcatt cctgcaagaa
     4561 caaggaagaa agacagaaag aagaaaaaga cccaacggcg aaaactctca gttctcggta
     4621 aaaaaccgct tgtcggttct gcgatacgta aaatggcttc taactggcca ctttttttga
     4681 ctggtaatgc gacatataaa agatcctcat tcaaagtagt acttttgcgc aaagcgctac
     4741 ctaggttccc accattcaat acggctttga tttcgggacg ggagtttcgc tgttcattca
     4801 atgtttcatt gtttgtgtca aataagatct ttccagtagc atccattaaa gtaatccgtt
     4861 cgttcgactg atggacgaat tcttccaaag cagttttgtt ttgcgctgct tggaggtttt
     4921 cgacatctaa ttgccggatc agcagttgtg ctttttttgt caggtagctt tcttgctgtt
     4981 ccacgatctg ctgttgaaaa taatgggaaa tcaattgcca gccaccaaaa aacaaactaa
     5041 ctaaaagaat aagaaagaag agacgttcat aaattttttt ccatttcatc gtttgggttc
     5101 ctgaaactta tagccaaatc ctctaatagt caccaagtac tttgggtgtt ttggatctcg
     5161 ctcgattttt tctcgcaaat gactaacgtg tacatctacg attcggcttt gtcctgcgaa
     5221 gtcaaaattc caaatgcggt ctagcaatgt gtctcggtcg atcacacggt cttttcgttt
     5281 catgaaataa acaagtaatt caaattcttt cggtgttagt tcaatcagat ggtcgtctac
     5341 ggtcacttga taattggtca aatcagcagt gatttgtcca atcgatagat attcaggctc
     5401 agcttcatct tttgattgct tacgaggttc aattcgtctg aatattgctt tcatacgggc
     5461 taatacttcg cgaggactaa atggtttggt caaatagtcg tcagcaccta tttctaggcc
     5521 aatgatccga tccacttggt catctttagc cgtcagcatc aaaatcggtg tttcattttt
     5581 ttcttgacgc agccgctgag tgatctccat gccatccatc gttggaagca tcacatctaa
     5641 aatgatgaaa tcaaatgatt gttctaatgc taattctaat ccttcgcctc catctgtggc
     5701 actggttacc ttgtagcctt ctttttccaa attgaacgtc aagagcgtta caatagaagg
     5761 ttcatcatca acaactagta tttttttcat tcaaatctcc tttaaatagg tttcattctg
     5821 tttatatttt agcatagaat cagtacaaag ggctatttta caaaagaaat aaatggtata
     5881 ataggaaggc actttgctga atagaaagga agagcagtca atgattggaa tcagtgcatg
     5941 tttaggcggt ctggcctgtc gctatgatgg acagaaaaaa gaagtaccag aattgatgga
     6001 actagttgaa actggagaag ctgtcatggt ctgtccagaa gtgatcggcg gattaccaat
     6061 tcctagagat cccgctgaaa tcattggtgg aaatggattc gatgtgtgga aagatcaagc
     6121 aaaagtatgg acgacaagcg gcgaagatgt aacggaagcg tacaaaaatg gcgcgatcaa
     6181 agcctatcaa aaattgcagg aaaaacacat tgatctagtc atcatgaagg aaaaaagccc
     6241 ttcatgcgga acccattcga tttatgacgg aagtttttcc ggaataaaaa aaaatggtgc
     6301 cggagtggct actgcttatt ttattcagca aaaaatgact gtgctctctg atgaagaatg
     6361 gctgaaaact agaggtgagc agaatggaaa tcgaaaagac caatcgaatg aacgcgctct
     6421 ttgaatttta ttcgacctta ttgacagaaa aacaaatgaa ctacatggaa ttatattatg
     6481 cagatgattt ttcattaggt gagatcgcgg aagaatacga agtaagccga caagcagtat
     6541 atgacaatat caagcggaca agcaagatct tggaggatta tgaaaaaaaa ctccatcttt
     6601 tttctaatta tattgttcga gaacaggtat tggaaaatat gactgcatat attacagata
     6661 agtacccgga agataaaaaa ctacttgaat atgtacagca aattcaagca atcgaagaat
     6721 aaataaaagg aatgaagaaa atatggcatt tgaaagctta actgaacgtc tccagcaggc
     6781 gatgggaaaa ataagaaaaa aaggaaaagt atcagaagct gacgtaaaag aaatgatgcg
     6841 ggaaatccgc cttgccttac tagaagccga tgttaacttg caagtagtca aagattttac
     6901 taagcgcgta cgcgaacgtg cggtaggggc agaagtgctg gaaagtttat cacctgcaca
     6961 acaaatcgta aaaatcgttg atgaagaatt gacgatcaca cttggttctg aaacagcaga
     7021 gttaaataaa tcaccaaaaa taccaactgt tatcatgatg gctggtcttc aaggggcagg
     7081 taaaacgacc tttactggta aattagccaa ttatttaaag aaaaatgaaa atgctcgtcc
     7141 gctcctaatc gcaggggacg tatatcgacc agctgcgatc gaccaattga aagtattagg
     7201 tcaacagcta gacgttcctg tatttgatat gggaactgaa gtcagcccgg tagagatcgt
     7261 ccgtcaaggg atggagttag caaaagaaaa gaaaaacgac tatgtattga tcgatacggc
     7321 aggacgtctg catatcgatg aaacattgat ggatgagttg aaacaaatca aagaattgac
     7381 gcaaccaaat gagattttgc ttgttgtcga tgccatgact ggtcaagatg ccgtcaacgt
     7441 agctgatagc ttcaaccagc aattaggtat tacaggggtc gtcatcacta aacttgatgg
     7501 ggatactcga ggcggggcag cactatcgat ccgctcagtg acaggcgcac caatcaaatt
     7561 catcggttct ggtgaaaaat tgactgactt agaagtattc cacccagatc gtatggctag
     7621 ccgtatcctt ggtatggggg atatgctgac attgattgaa aaagcgcagc aagactacga
     7681 cgagaaaaaa gcggaagaac ttgcacagaa aatgcgggaa aattcattcg atttcaatga
     7741 ctttattgaa cagcttgatc aagtgatggg tatgggccca ctggaagatc taatcaaaat
     7801 gatcccagga atgaatcaag tccctggaat cgaaaacgtc aaggtagatc caaaagatgt
     7861 tgaacgcaaa aaagcgatgg tttattctat gacgccagca gaaagagcaa atcctgatct
     7921 gttgaaccca agccgtcgtc gccgaattgc tgccggttct ggaaacagtg ttgttgaagt
     7981 gaatcgaatg atcaaacaat tcaaggaatc aagaaaaatg atgcagcaaa tgtccaaagg
     8041 ggatatgaac atcccaggta tggaccaaat gtttggttca ggcgtcaaag gaaaactagg
     8101 caaaatggcg atgaatcgaa tgatcaagaa aaataagaag aaaaagaaaa aacggaaata
     8161 agatgaaaaa aacagcttcg gtacagactg aaaatgtcta ctgccggagc ttttctatca
     8221 gcaattttta ttctttacta atacaattac tctaaacgaa gaggtggcac agatgagatg
     8281 tccgtggagt gaacaaacag aaagtatgcg gcaatatcat gatactgtct ggggtgttcc
     8341 tgaatatgat gaccaaaagc tgttccgaaa attgatgctt gatattaatc aagccggatt
     8401 gagctggcag acgattttaa ataaaagtga agcattcgat gaagcatatg atcgattcga
     8461 tatcaaaaaa gtcgcaaatt atggagaaac aaaaatcagt gaactaatgg aaaacaaagg
     8521 aattattcgt aaccgaagaa aaatcgaagc agccgtccaa aacgccaaag cagtcttgaa
     8581 aattcaagaa gaaatcggca gtttttcaga gtatctttgg tcgttttcta atggaaaaat
     8641 cgttgattct tcttatcatt cacaagaaga agtcccaaca acaagtcctc tttcagatga
     8701 attagcaaaa gatctaaaga aaagaggatt taaatttatc ggaagcacaa caatctatgc
     8761 ttttttagaa gctgtcggga tcatcaatga tcaccttgac agttgtttcc gcaaagaaga
     8821 ggtcgtacgt atcggcagaa agggcgaata aagcatgcga aaaatcatat tttatggtgc
     8881 tataagttta gatggctatt tggcaacgaa aacggatgat cttcaatggc tttttgacac
     8941 tccaacaggt aagcaaacta cttatgaagc tttttatgaa acaatcgata cgaccatcat
     9001 gggcagaaaa acttatcagg aagcaaaaaa atatttggag accgaagcaa tttatccaga
     9061 gaaaaagaat ttcgtttttt ccacccacgc acaattacaa ttgccagatg ctgaggtcgt
     9121 aaacgaagat cctgttgctt ttctaaaaaa tctacaaatg acagaaggaa aagatatctg
     9181 ggttgtgggt ggcggtcgat tgatcaaacc gctattggaa catcaaatga ttgatgaatg
     9241 gtatatccag attacccctg tattattagg ggacgggatt cctttattcc aaaaaggaac
     9301 atatgaagcg cgttttgaaa tgctagacac gacacgattc ggtgaattta tcgaattgca
     9361 ttatgtacga aagtagaagt aaataaaata aaaaaggcaa ctatatgcag aaatgtcctg
     9421 tacatagttg ccttttttat taccgttatg ctttttactc tgttaatttg ttagaaaaat
     9481 ctagatacgc atctaaaatc gtttggaaga aacttaatgt tccttcaacg atattaccat
     9541 tttcatcaat tagattcgca acgtttccga tatatgcttc cggttgctgc aaggttggca
     9601 cattcaaaaa gacaagtgat tggcgtagat ggtggtttgc gccaaagccg ccgatcccac
     9661 atggtgatgc agtaacgact aaacctggtt tatgatccca aacactgtgt ccataaggtc
     9721 ttgagcctac atctaaagca tttttcaata ctgctggcac agaacggttg tattcaggag
     9781 taacgaaaac gacaccgtct agtcccctca cttcctcacg aaaacgagtc cattcagcag
     9841 gagcctgttc tggagtttcg agatcctcat tgtaaaatgg cagatcatcg atttttacaa
     9901 atacactttc gtacccttca ggaagcaatt tcgcaaaagc ttctgctact tttttgttgt
     9961 aagaatcttt tcttaaacta ccaataaaaa atccaatttt ttttgtcata cgatttttcc
    10021 tccttcaaac tatctataat aatcttacaa aagataagta actggagcaa ataaaaacac
    10081 ttactgacaa aagctacata gaagatagat tacttatttt taacaaaaaa gaagtctaaa
    10141 atcttctatt ttctaaaaaa aatcgaattt aatcaagctg taaagaaaaa aagctttaca
    10201 gggtaaaaaa aaactgttac actacatctt gtgaataaaa caatggaggt ggaaataaat
    10261 aatggcagtt aaaatccgtt taaaacgtat gggttctaaa aagagtccgt tttaccgtat
    10321 cgtcgtagct gattcacgtt ctcctcgtga tggacgtttc atcgaaactg taggtactta
    10381 caatcctttg aaagaccctg cagaagtagt tttaaaagaa gacttagttc ttgactggtt
    10441 atcaaaaggt gcacaacctt ctgacactgt acgtaacatc ctttcaaaag aaggcgttat
    10501 gcaaaaacac cacgaagcta aattctcaaa gaaataaggt gaccggatat gaaagattta
    10561 agcgacttag tcttaacaat cgttcgtccg ctagtcactt atcctgatca agtcaagtta
    10621 gacgtcgtag agtcgaaaga cttttacgag tacaatctaa cagtggctcc tgaagatatt
    10681 ggccgtatca ttggaaaaca agggcgtgtt gctaaagcaa tccgcacaat cgtttacggt
    10741 gttcggatca atgcaccgaa aaaagtgcgt ttgaatatca tcgatgataa ggaaaagtaa
    10801 ataaacgtgc tgtactcgaa ttttcgagtg cagctttttt gtttttttta ttaattgaag
    10861 aaatgattat ttggcaaaac aagcaagaaa gcgtatcctt ataggaggag ggagtgaaca
    10921 gccaatgaga aagaaaatgt tatttattcc atttgctcta ttgttaagcg cttgtagttc
    10981 ggttactcaa gaatcagaca atacacatct atcaagcagt atatctgaaa ctgttaccag
    11041 tccagaaaaa agtagtacca aaacggcttc caccactgaa caaacaatgg ctacctcaaa
    11101 taatgagaaa aagactgctt tagaccagtt gaaagaacaa cagccaaatg ttcccatgcc
    11161 tctagatgta ccagtctctt caggctatct caatatcgca gcgacacata caaagcaagg
    11221 ttattctatt ttatattaca gaacagatcg tcccctcggg ttgaatgctg acgaattgaa
    11281 tcaagaaacg cctattgcaa cctatctata tcaatatggg tttgcttcta gtcaagaaac
    11341 aatccaagtt ttgcagccat ttgagatcga cacaaatggt cagcaagtgg acttaggttc
    11401 tcgaatcaca gggtatcagc aaggtgcagc aggttcaagt tttcttgaat ggcaagaagg
    11461 aaactggcgt attcgaatcc gtgggaataa tatagaaggc caagatccct tactattagc
    11521 taaagaaatc gtagcttatt tggaagaaaa cagtctacca gcacctgaac aatttggtaa
    11581 aattactgtt gatatgggtg atacgacgaa tcgagccgta gaagtaagct ggcaagaacc
    11641 gaaaaatgcc tatacgatca ctcatcaaga tcctatgtct gcgttgaaga tggcagtttc
    11701 tatgaagagg ttgtgaaatc agcgcgctga tctgagccca tcatttaact ccaagtcaca
    11761 ggaagtaatc aaaaatagca cgccgcattt ttgcataatt ttggcttaat accttaaaag
    11821 aaaactagaa ttgcctcttc acacatgcta aaataaaaca gacaaaaaag aaatggggat
    11881 ataagttgac tgaatacttg aatgtaggaa aaatcgtaaa cacacaaggt atcaaaggcg
    11941 aagtccgtgt tatctcaaca acggatttcc cagaagaacg ttacaaaaaa ggaacggtgc
    12001 taacactatt ccaagacgga aaagcacctg tggaattaac agtcaaaagt catcgcaaac
    12061 ataaaaattt tgacttattg agttttgaag gacatccttc tatcaatgat gtggaaaaat
    12121 atcgggatgg catattaaaa gtgtcaaaag acaactctgt agatctagct gaaaatgaat
    12181 tctattacca tgagatcatc ggtctgcatg tattagaaga tggaactgag ttaggtaaag
    12241 taaaagaaat cttgtcacca ggtgccaatg atgtttgggt cgttcaacgt ccgaaaaaag
    12301 ctgatatatt gcttccatat atcgcttctg tcgtcaaaga agtcgattta gaagctggag
    12361 tggtccgtgt agaaattcca gaaggactga ttgacgatga agattgatgt attgacttta
    12421 tttccaagaa tgtttgaagg tcctatgggg gaatccatca tcggtaaggc tgtagataaa
    12481 ggattattag atatcaatat ctctaacttc cgcgattatt cagataacaa acatcagaca
    12541 gtagatgatt atccatacgg cggtggagca ggtatgctgc tcaaagtcca gcccatctat
    12601 gataacatcc aagccattga aaaagctgca ccggatgtca aaaaaagagt aattcttctt
    12661 gacccagctg gtaaacgatt cgatcagaaa atggcagaag aattttcaca agaagaacac
    12721 ttagtattca tttgtggtca ttatgaggga tatgatgaac gtatccgcac attagtgaca
    12781 gatgaagttt ccttaggaga ttatgtcctt actgggggag aattaggtgc gatggtcatg
    12841 atcgatgcaa cagttcggct tttgccagat gtgttaggga atcagacttc agctcaaacg
    12901 gattcttatt caaccggttt attggaacat ccccaatata ctcgcccggc agaatataaa
    12961 gggatgaaag taccagaagt attgacaaat ggaaatcata aattgattga agaatggcaa
    13021 ttaaaagaat cactaagaag gacctatcaa cgtcggcctg atcttttgga aacacttgaa
    13081 tggacacctc aaatgacgaa atttttagaa gaaatcaaga aagaagaaca agaaaaaaca
    13141 gcagatgaag ccaagtgact ttttctaaaa tcttctttac agtaggttga taatatgtta
    13201 gtattttatg gtgagtgcaa aagcacttaa ctattacgat attccgctgt gagaaaagct
    13261 cataagaata tttggaataa ggagaagaaa accatgaatc cattaatcga agaattaaca
    13321 aaagaacaat tacgttctga catcccagcg ttccgccctg gtgacactgt gcgtgttcac
    13381 gcaaaagttg ttgaaggtac tcgtgaacgt atccagttat ttgaaggtgt tgttatcaaa
    13441 cgccgcggtg ctggaatcag cgaaacttat acagtacgta aagtttctaa cggagtgggc
    13501 gttgaacgta cattcccatt gcatacacca cgtgttgcaa aaatcgaagt agttcgctac
    13561 ggtaaagtac gtcgtgcaaa attgtactac ctacgtgcat tacacggaaa agctgctcgt
    13621 atcaaagaaa tccgtcgtta atcattgtta tcagactgag aaagaaccct tggtattcaa
    13681 gggttctttt tttattatct gtttttttat attaacgatt tctatttggt acatttttga
    13741 ttaataaaaa ggggcaaaaa aggggcaaca agttacagtt tttctatatc ttctttcaat
    13801 ttttctttta ctccttttgt cacatgcaaa taaatctttt cagtgatatc actattttcg
    13861 tgcccaacgc gatcctgaat agcatacaga ggagttccta gttccgctaa ttttgaaata
    13921 tgggtatgtc taaaaatatg tgaactaagt tttttatcaa tcttcatgtc agctttatga
    13981 tttctcaaat aggtgttaat tgctgttagt tggaaaggag ttcctttcgt tgtttgaaat
    14041 agaaattgac ccttcggatt taattctaga agctcattgt aaatagctat cgcttttttt
    14101 ggtaaatcaa tttctcgcat tccagcagca gtttttgtag aatcagattt tttcatatca
    14161 gctattgaac gttctctata catcatcgtc ccatttataa ctactgatgc attattattg
    14221 gtaatgtgta catcgtcttt acttaatgcg attgcttctc caggtctcat tccagtcaaa
    14281 tatagccact ggaaaaggag agaatatctt ttattgtgtg aagttgtgaa atctactagt
    14341 cttttatatt catcatcttc taaaaactta tctttaattt ttatcgtttt tgactctcgt
    14401 ttataatcaa taactacttc gttgatagga tttttttcta catagccttt tttcatagcg
    14461 tatgaaaaaa gaagattcaa tttggattta attacactga cgtacttatt tgacaagtca
    14521 tctttatata tcatatcttc aaaaatatta tttaaatcta ctgtattaat accagaaacg
    14581 atgtaagtct caggcatctt cttctttata gtgtttaaga tattattcgt aggataatat
    14641 gtagactcct taacttgcct tttgtaaata actaaccatt cttcgacaag ctcatgaaat
    14701 gttagattag gcttctggag tgttttttca tttagtttta tatcaatttt tttatttaac
    14761 tccagtaatg ccactttctg agtttctcga gacttatttt tataagtaat gctcactttt
    14821 tttctttttc tagtttttgg atcaatatat cgttcattat atttatatac tttttttccg
    14881 tttttatcag ttttggtttc aatccacatt gttttcatct cctatttgtt gctataatag
    14941 gaatggataa gtaagccgat tatagcaggt ttatttttca tcacgtccac aaactttggc
    15001 gagggagggg gcgtgttttt gtattttaac gatttatatt gaatgttgat tcagcacttt
    15061 cagcaactgt actataatca gacggattat ataacactcc ggtttttatc gttacttttg
    15121 taatatcgtt aactttagat ggatctaagt aagctaaact ataaaattct ttactttcag
    15181 tttttttgaa aaacgagttt gttgtcattg tatgcataag atcgtctgaa ctgaaatcaa
    15241 tttgttggcc gttatcaagt atggcgtgtg caagcccatt ataaccataa tctacttcag
    15301 aattgttttt aactttgaat gcaatttgta aaccataaaa atttccttca ctatctttgt
    15361 gattatttgc atcgtagtaa ccagcagcgc tagggtcagc ataattggaa tacttgagta
    15421 gtttgatatt agatatataa atgccgatat caccaagagg aatatctgat tgtgggcttg
    15481 ccgttccaat taattcaatt ttgccacttg tatctctttt ttctttggtc cattgaccaa
    15541 ctttgaggag ggggccatca acctcctgtg aatttttaga agactgtgtt gtactttctg
    15601 ataacgtaac tgaagagtct ttttcgtttg ttttgccagt attatctaca acattagatt
    15661 ggctacatcc ggtaagaaaa aaagcaccaa atactaaccc taaaatcact tttttcattc
    15721 tttattctcc gtttctatga tattattttt atgtaggatc ttagaaacga gatcttagtc
    15781 cgtgttgcag cacgggcttt tttctttata acttttttag agattatagg caaaatagta
    15841 ggacacaaaa atatattatt gtattccgta tttagaaaat cctaattgaa cttcaccaga
    15901 agttttttgc tgtgttgtac gcaatgcttc ttcttcagac attccattct gtactttcca
    15961 tgcaacaggc gacatcccgt atttgttaac aaaatcagta agtgataaag tgtcagcgtc
    16021 ttgctgaccg cttgtttgtt ggtcttctgg attttgttga gatgctgctt gttgttcttt
    16081 ctgatcttga ctgataatat tgccagcatc atctgtagtc aatccatttt cataaagggc
    16141 cacgccgaaa gcttcccact ctttgttgga ccaatttgca cgatcagctg gagttgactg
    16201 taaagtgcgt tgtttcatct gttcatatgt ttcttcttga ggtgcggttt ggattgtatc
    16261 ctgactggag cttataactg ttgggctagg ttccgccgta gcttggttag agcttgtaac
    16321 tgttgaacta gaatctgtct tagatgtaga tttgctagta gaggaactgg tttcagttgt
    16381 ttcttttgtt ttacttactt ttgtttcttg gttagaagtg gcatctgttg attcagcttt
    16441 tttattattt gaacaagctg aaagtagcag agcagtactt aacaacaaca taacgctaac
    16501 ttttttcatt ttataattcc tctttctcgt tgtaatatgt gtgctaacac gggctttttt
    16561 atataagaaa acgataagcg ctttctggaa gtccgtaaag attctttaat tcatcgattt
    16621 ttttgggata ttgatcatta tcttctttat aaagagaaac aatgagatta gcagcaaagc
    16681 aattagcttc gctttcagat ttgcttctag atgttcttgt tgatacatag taactggata
    16741 agccgcgatg aaaaatagcg tgacctaatt cgtgagcgca aatgtagaat ctttcctcag
    16801 agtctcgcag ttcatcattt aagaagatta ttgcacgacc tctaatttct tgaaactgtc
    16861 ctttgggatt ttcgataaaa ggaacgtatt gaattttaat gcccatcttt tcacaaatat
    16921 aaaaaggatt agcagaccgg tatttccgct tcaactcctc gactaaatta atcgtatcca
    16981 tctccataag ctcacatctt tttgcctttt tctttgtctt ctttcacaat atcccagaag
    17041 gttccaataa ggatatcttt tacgcgttgg acctgttcag gcgtcagtgt ttctccgcca
    17101 taagacatat tgacatttga atctagtaat ttatcaagtt caactacttc atcttttgtt
    17161 gcccagtcag gaacttggtt tctaccgagt aaataatcag tagttacatt aaaataatct
    17221 gctacaagtt gtagtctttt agtactagga gttctatttt tccattgata aatcgtgttt
    17281 ttaggtatat ttaattcttc ttccaattga gttatactta tgcctctttt atgagctaat
    17341 tcctttattc tatctagtaa attcattttc gcacctcaca agctacgaaa acactaataa
    17401 aaaaattagt taaaggtgtt gacaactaat aaaattgtta gtatactgtt ttcgtaagct
    17461 aaaatattag ctaaaaagac ttgataacta ataaacactt cacggtcggc aaactctgaa
    17521 atgtaaatta ctaggcattt ctgtgtctta tttagctata tctatatact aataaaaata
    17581 ttagttattg tcaacgaata ttagctaatt ttttagctta cagactattt tttagaaagg
    17641 agctattttt atgtctgaga atttagactt aaaaattcga gcggagatga gaaaaagaag
    17701 aatgactttc aaagaactag ctgcgcttgt tggtatttca ggagcttatt tatcagatat
    17761 tctaaacggc aatcgtgatg gaaagaaagc acaacagcat atcgaaacag tgaaaaaaat
    17821 attggacatc cgataggggg ggtaggcgat gacaaaacta aaaaaacaag attttgtaaa
    17881 aaaatacaat tattctccat ctacttatca acgtcgaatg tcggaactaa aaaatacagc
    17941 aattttctca gcggcgtatg aacgggtcac aggacaagaa gtttggatca atacagaatt
    18001 atacgataaa tttttgtctt tcaaatccta taacaggtta cgcacaagaa agataacacc
    18061 taaagaattt atcgagaagc atttagttga tttataaaaa acagaacatt ttgagaggtg
    18121 aaggttaatg ggcaaattca acagagcgct agtgttcagc gcaccgctaa tcatctacgc
    18181 tttaggcct
//
LOCUS       DASHHB010000056        18080 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.058, whole genome shotgun sequence.
ACCESSION   DASHHB010000056 DASHHB010000000
VERSION     DASHHB010000056.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 18080)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18080)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18080
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.058"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            598..1287
                     /locus_tag="U2K71_002186"
     CDS             598..1287
                     /locus_tag="U2K71_002186"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002411958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7316218.1"
                     /translation="MKILIADDDKEIVELLSIYVHNEGYEAVKAYDGKEALSKIRTIP
                     DIELLILDIMMPEMDGMQVVKELRKESQIPIIMLTAKTTDMDKIKGLVAGADDYVTKP
                     FNPLEVMARVKSLLRRTKMQIAPDQPDVLEVNALIINKDSHEVKTIDGKEIQLTALEF
                     GILYLLASHPNRVFSADEIFERVWKQESIVSAKTVMVHVSHLRDKIEEATGGEKVIQT
                     VWGVGYKIDAR"
     gene            1274..2482
                     /locus_tag="U2K71_002187"
     CDS             1274..2482
                     /locus_tag="U2K71_002187"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM7316219.1"
                     /translation="MTQDSKRATEKRRRINLTSKEISELLAEGIITIILLLLLNVSIL
                     VVISSVINSSPSLTNAIWDSKNIFAERLNTDLFWNGRNFIIPFFFLLDIGVLYWRLIR
                     RYRQMQLRHIISELHYIANGNYDHRIPFELSGDLSRVVTSINGLVDSTVAAIEDERKI
                     EKSKDELITNVSHDIRTPLTSIIGYLGLIEDGQYHSEEDLLKYTHTAYIKAKQMKSLV
                     DDLFEYTKVRQPAVPVNFSAFDMIQLIEQLAADFELEASKKNIQILVQSKVDSLIMDG
                     DTEKLVRVFNNLLTNALKYGKGATKIVIEVERIGSEVVATVKNNGAMIPQQAIDNLFD
                     RFYRVEESRSQATGGTGLGLAIAQSIVALHGGYIYAKSDKQWTSFIMHLPIKKNEKLP
                     IDTQEVIDES"
     gene            2776..4047
                     /gene="serS"
                     /locus_tag="U2K71_002188"
     CDS             2776..4047
                     /gene="serS"
                     /locus_tag="U2K71_002188"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HEM7316220.1"
                     /translation="MLDVKMMRQNIEEVQEKLMTRGVKKETLAEFMELDENRRKLLVK
                     SEELKKYRNDVSAEIAQLKRAKEDASAKIAEMKEVGGNIKALDTEIAEVDEKLKQIAT
                     TLPNLPHDSVPVGTDEEDNVEIRRWSEPRTFAFEPKPHWEIAENLDILDFERGAKVAG
                     SRFVYYKGLGARLERAVYNFMLDQHIYEHGYTEMITPYIVNSKAMFGTGQFPKFKEDV
                     FQLENTDLTLIPTAEVPLTNYYNDEILDGADLPIYFTALSPAFRSEAGSAGRDTRGLI
                     RLHQFNKVEMVKFSDVEHSYEELEKMTADAEDILKKLHLPYRVMALSTGDMGFSAAKT
                     YDLEVWIPAQDTYREISSCSNCEDFQARRAMIRYRDNDGKIHYTHTLNGSGLAVGRTV
                     AAILENYQNEDGSVTVPEALVPYMGNLKVIK"
     gene            complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="U2K71_002189"
     CDS             complement(4596..6080)
                     /gene="guaB"
                     /locus_tag="U2K71_002189"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HEM7316221.1"
                     /translation="MSNWETKFTKKGFTFDDVLLIPAESHVLPNEVDMSVQLAKNIKL
                     NIPIISASMDTVTDSKMAIAMARQGGLGVIHKNMTISQQADEVRKVKRSESGVIIDPF
                     FLTPQHLVADAEELMSKYRISGVPIVETLENRKLVGIITNRDMRFVTDYHMPIADVMT
                     KDNLVTAPVGTSLKDAEKILQKHKIEKLPIVDNEGRLSGLITIKDIEKVIEFPNAAKD
                     EHGRLLVAAAVGVTSDTFERANALLEAGADAIIIDTAHGHSAGVIRKIQEIRSTFADA
                     TLIAGNVATAEATKALYDAGVDVVKVGIGPGSICTTRVVAGVGVPQLTAIYDAASVAR
                     QYGKAIIADGGIKYSGDIVKALAAGGHAVMLGSMLAGTDESPGEFEIFQGRRFKTYRG
                     MGSLGAMEKGSKDRYFQGSVNEANKLVPEGIEGRVAYKGSVADIIFQMIGGLRSGMGY
                     VGAANLQQLRDEAQFIQMSGSGLKESHPHDVQITKEAPNYSVES"
     gene            complement(6585..7280)
                     /locus_tag="U2K71_002190"
     CDS             complement(6585..7280)
                     /locus_tag="U2K71_002190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1129 domain-containing protein"
                     /protein_id="HEM7316222.1"
                     /translation="MEPEQLRAIVKENNSLETKLTKRNQQYIFDLKKSLEAANLSEEE
                     KVKALHDILPTLVAEQKGGRTARQLFGTVSERTEAIINKPAEVKNSSTPLLMWLDNTL
                     LLLGLLSIMIAITGLFSRGTTPAYGFSTLVLGSAIGGWVFYMMYKYIYQYEYPGADRS
                     KKPGMGKTLLILGGTTLLWVVLFSATTLLPPVINPVLDPVVVIIIGATALAIRYYLKK
                     KYGIIGSLSVPRR"
     gene            complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="U2K71_002191"
     CDS             complement(7370..8470)
                     /gene="ychF"
                     /locus_tag="U2K71_002191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-regulated ATPase YchF"
                     /protein_id="HEM7316223.1"
                     /translation="MALTAGIVGLPNVGKSTLFNAITKAGAEAANYPFATIDPNVGMV
                     EVPDWRLQRLTELVHPKKTVPTTFEFTDIAGIVKGASKGEGLGNQFLSHIRQVDAICH
                     VVRCFDDDNITHVEGRVDPLEDIDTINLELVLADLESINKRYTRVAKVAKTKDKDAVA
                     ELAVLDKIKPVLEEGKSARTIEFTEEEQKIVKSLFLLTTKPILYVANVSEDDVADADA
                     NHYVQEVRNFAAEENAEVIVVSARAEEEIAELDEEDKAEFLEALGIEESGLDQLIRAA
                     YDLLGLATYFTAGEQEVRAWTFKKGIKAPQAAGIIHSDFERGFIRAETVSFEDLDHYG
                     NMHAAKEAGRVRLEGKDYVVQDGDVMLFRFNV"
     gene            complement(8479..8670)
                     /locus_tag="U2K71_002192"
     CDS             complement(8479..8670)
                     /locus_tag="U2K71_002192"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF951 domain-containing protein"
                     /protein_id="HEM7316224.1"
                     /translation="MYDLHDIVEMKKPHACQTNRWEVIRMGADIKIRCMNCGHIVMMT
                     RRDFEKKLKKVIEKNAEKE"
     gene            complement(8691..9581)
                     /locus_tag="U2K71_002193"
     CDS             complement(8691..9581)
                     /locus_tag="U2K71_002193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HEM7316225.1"
                     /translation="MKKSKGLGRGIDALFQDLENLETVDVKDDTVVQLPLNELRPNPY
                     QPRKTFEEVSLQELANSIEQSGVFQPIIVRRSAVKGYEIIAGERRFRASKLAEKETIP
                     AIVRDFDEEAMMQVAVLENLQREDLNPLEEAEAYEMLMKNLKLTQAEVASRLGKSRPY
                     IANYLRLLSLPDLVKEMVQDQRLSMGQARTLLGLKNKTLILKLANRAVTENLTVRQLE
                     QLVSELNQNDGKEKKKIPRLAKEKPYYIRESEDRLMDKFGTSVAIQEKNGKGKIEIEY
                     LSQKDLTRILDILNIQFDEE"
     gene            complement(9568..10335)
                     /locus_tag="U2K71_002194"
     CDS             complement(9568..10335)
                     /locus_tag="U2K71_002194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM7316226.1"
                     /translation="MAQIISVANQKGGVGKTTTTVNLGACLASLGKKVLLVDMDAQGN
                     ATSGVGIRKPDVTRDIYDVLVNELPIDEATLITEHENLSIVPATLQLAGAEIELTSMM
                     ARESRLKGSLAEVSSQYDYILIDCPPSLGHLTINSFTASDSILIPVQCEYYALEGLSQ
                     LLNTVRLVQKHFNPELEIEGVLLTMYDARTNLGNEVVEEVRKYFREKVYETIIPRNIR
                     LSEAPSHGKPIIDYDPRSRGAEVYQALAKEVVSREEK"
     gene            complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="U2K71_002195"
     CDS             complement(10347..11060)
                     /gene="rsmG"
                     /locus_tag="U2K71_002195"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(527)-N(7))-methyltransferase
                     RsmG"
                     /protein_id="HEM7316227.1"
                     /translation="MIPEEFRQSLAEKGIELTDHQMNQFAIYYRTLVEWNEKMNLTAI
                     TDEKDVYLKHFYDSLTLAFEVDFTQPLSLCDVGSGAGFPSIPLKIAFPELQITIVDSL
                     NKRITFLSELTRLLGIDASLYHDRAETFGQNPHFRASFDLVTARAVARLNVLAELCLP
                     LAKQEGYFYALKAAKSEEELTEAKQAIALLGGKFLKEEAVTLPNVSDQRHIIVIQKKK
                     PTPKKYPRKPGLPNKQPIK"
     gene            complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="U2K71_002196"
     CDS             complement(11292..13193)
                     /gene="mnmG"
                     /locus_tag="U2K71_002196"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis enzyme MnmG"
                     /protein_id="HEM7316228.1"
                     /translation="MEHYEADAYDVIVVGAGHAGSEAALAAARMGSKTLLLTINLDMV
                     AFMPCNPSVGGPAKGVVVREIDALGGEMGKNIDKTYIQMRMLNTGKGPAVRALRAQAD
                     KHAYSTEMKHTIEKEPNLTLRQGIVEELIVEDGVCHGVVTSTGASYRAKAVVITAGTA
                     LRGEIIIGELKYSSGPNNSQPSIGLANHLKELGFAIERFKTGTPPRVKSSTIDYSVTE
                     EQPGDKEPNHFSFSTPDSAYKLDQESCWLTYTGETTHQIIRDNLHRAPMFTGIVEGVG
                     ARYCPSIEDKIVRFADKPRHQLFLEPEGLNTEEVYVQGLSTSLPEDVQVNMLHSIAGL
                     EKVEMMRTGYAIEYDVVEPHQLRPTLETKVVENLYTAGQTNGTSGYEEAAGQGLIAGI
                     NAALKVQGKEPLVIKRSDGYIGVMIDDLVTKGTNEPYRLLTSRAEYRLLLRHDNADLR
                     LTEMGHRVGLVKEDQYKNYLKKKEAVEQEIKRLMNVRIKPTEEVQDFLATINAAPLKD
                     GVLASDFLRRPEITYAELLRFIPENEELTRKEIEQVEIQIKYEGYIKKAMEKVEKLKR
                     MEAKKIPENIDYAAINGLATEAKQKLQKIQPETIAQASRISGVNPADLSILMVYIEQG
                     KIAKVSEPA"
     gene            complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="U2K71_002197"
     CDS             complement(13210..14607)
                     /gene="mnmE"
                     /locus_tag="U2K71_002197"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA uridine-5-carboxymethylaminomethyl(34)
                     synthesis GTPase MnmE"
                     /protein_id="HEM7316229.1"
                     /translation="MAEITLEFDTIAAISTPPGEGAISIVRLSGDQAVQLADKVYQSG
                     NKRLSEVPSHTIHYGHIVDPKSNQLVDEVMVSVMRAPKTFTREDVVEINCHGGIVVVN
                     QILQLLLREGARLAEPGEFTKRAFLNGRVDLSQAEAVMDLIRAKTDKAMGLALNQLDG
                     NLSALIRSLRQEILETLAQVEVNIDYPEYDDVEELTTKLLLEKAQMIQQRIQALLATS
                     KQGKVLREGLSTAIIGRPNVGKSSLLNHLLREEKAIVTDIAGTTRDVIEEYVNVRGVP
                     LKLIDTAGIRETEDVVERIGVERSRKALAEADLILLVLNQSEPLTAEDEQLLEATSGL
                     KRIILLNKTDLPAQLEQEKLKKLIENEPVFSISVAKNDGLDRLESAISDLFFSGETGE
                     RDATYVSNTRHIALLEKASLSLEEVIAGIDSGMPVDLVQIDMTRCWDYLGEVVGDSVQ
                     DELITQLFSQFCLGK"
     gene            14906..15289
                     /locus_tag="U2K71_002198"
     CDS             14906..15289
                     /locus_tag="U2K71_002198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316230.1"
                     /translation="MVALSKLYTFERRKYVSPFRSAALEEESDPRINDVVLSTGNINQ
                     KYIVRDIVFVVECLEADLFDPSFPKDDLFLTTKRKLKTKVLEYGANAVINCHFEYQQK
                     KRDGKYFLELFAYGTVIQFIQTTIG"
     gene            complement(15457..16215)
                     /locus_tag="U2K71_002199"
     CDS             complement(15457..16215)
                     /locus_tag="U2K71_002199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Jag N-terminal domain-containing protein"
                     /protein_id="HEM7316231.1"
                     /translation="MPIYEAATIDEAVQLGLADLGLAKDQVEIEILEEGKKGFLGMGR
                     KNARLSIQPSISEKVSETVEQTVEEILEEEPTAEIEVVNETILEEKTEEVSELGDEAA
                     LTELAVYLTEISKQLDAPALVKMERESNNMVVFHLETQKQGILIGKHGKTLNALQYLA
                     QVFIHRLARNKLSVVVNVGNYREKRQEIIQRLADRTAEKVKRTGQPVFLEPMPAFERK
                     QIHAALSKDEHVKTHSEGEEPYRYLVVEPAKKYY"
     gene            complement(16233..17051)
                     /locus_tag="U2K71_002200"
     CDS             complement(16233..17051)
                     /locus_tag="U2K71_002200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertase YidC"
                     /protein_id="HEM7316232.1"
                     /translation="MKKYKRLLLMAGIVSLVIVLSGCGTGEVSAQSTGIWDRYIVYYF
                     AEAIKALSFGNAGIGIILFTLIIRVILLPLMHFQTKSMRKTQELQPKLKALQQQYSSR
                     DPETQSKLREEQQRLYAENGVNPYAGCLPLLVQMPILMALWQSISRVPELKQGHFLWL
                     NLGNADPTFILPILAAIFTFASTYLSSMSQIESNASMKIMNFVMPVMILVMGVNLASG
                     LSLYWVVSNAFQVVQTLLINNPFKIRKEREEEARRIRERERALKKAMNPKKKRK"
     gene            complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="U2K71_002201"
     CDS             complement(17048..17404)
                     /gene="rnpA"
                     /locus_tag="U2K71_002201"
                     /EC_number="3.1.26.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease P protein component"
                     /protein_id="HEM7316233.1"
                     /translation="MRKAYRVKKEREFQAVFHTGDSFANRKFVVYRLEKPGQPHFRVG
                     LSVGKKVGNAVNRNEVKRKIRAAVYSIKEEIDPELDFIVIARPSVKGLTYDEVRSNLV
                     HVLKLAKIIDREEKSK"
     gene            complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="U2K71_002202"
     CDS             complement(17522..17656)
                     /gene="rpmH"
                     /locus_tag="U2K71_002202"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003397591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L34"
                     /protein_id="HEM7316234.1"
                     /translation="MKRTYQPNKRKRQKVHGFRKRMSTKNGRRVLASRRRKGRKVLSA
                     "
BASE COUNT         5032 a         3739 c         3297 g         6012 t
ORIGIN      
        1 ttcatcagga gtgtttcccg attcgttttt ggctgccgta gcaactttta tatcatatca
       61 gagagacaag taattgtcaa ctctttttta aaagtttttt cgatttcgtt tttcgaaatc
      121 ttccagcaac tcatttatca taacttatcg tttgttattt gtcaagaact tttttgaagt
      181 ttttttcgat agaatcaagc ttcgcaagtg gttctttttt aacgactcag ttatcttatc
      241 atgtcatttc caacatgtca acaacttatt ttttattatt taataagtta ttggtgttct
      301 tttgaacaaa taataatata tcaacatcac atgtgaaagt caataagaaa atgcttattt
      361 atcaacattt ctagcttaca tgatcattgg ttagttctgg tcctttttcg ttcgtcttta
      421 taactccgag agacgtaaaa tccttttgtt ttcagtttaa tgttcatgtt cttgtcttat
      481 tcacaccaac tagatgtctc accttctaaa attttcctta acaaagtgct gcttttatct
      541 atttctgctt ttttttgcta gaataatagt aacaatatga ctctggagga tttatttatg
      601 aaaattttga ttgctgacga tgataaagaa atcgttgaat tattaagtat ctatgttcac
      661 aatgaggggt atgaagcagt aaaggcttat gatgggaaag aagcattatc taaaatcaga
      721 acaatccctg atattgaatt acttatttta gatatcatga tgcctgagat ggatgggatg
      781 caagtagtga aagagctacg aaaagaatcc caaataccaa tcattatgct gacagcaaaa
      841 actactgata tggataagat caaaggactg gtcgctggtg cagatgatta tgtgaccaag
      901 ccatttaatc cgttagaagt aatggctaga gtaaagtctt tattacggcg tacaaaaatg
      961 cagattgccc cagatcagcc tgatgtatta gaagtaaatg cattaatcat caataaagac
     1021 tctcatgaag ttaaaacaat cgatgggaaa gaaatccagt taacagcttt ggaattcggt
     1081 atcctctact tgcttgctag tcatcctaat cgagtattta gcgcggatga aatttttgag
     1141 cgtgtttgga aacaagaaag tattgtctct gctaaaactg tcatggttca cgttagtcat
     1201 ttaagagata agattgaaga agcaactggt ggtgaaaaag tcattcaaac cgtttgggga
     1261 gtgggctata aaattgacgc aagatagtaa acgagcaact gaaaaacgcc gtcggatcaa
     1321 tttgacttca aaagaaatca gcgaattgtt agcggaagga atcatcacga ttatcctttt
     1381 gcttctatta aatgtttcta ttctggttgt tatcagttcg gtgatcaaca gttcaccttc
     1441 tttgacgaat gcgatttggg attcaaagaa tatttttgca gaacgattga acactgattt
     1501 gttctggaac ggccggaatt ttattattcc ctttttcttt ctattagata ttggtgtttt
     1561 gtattggcgc ttgattcgaa gatatcgcca aatgcaattg cggcatatca tcagcgaact
     1621 tcactacatc gcaaacggaa attatgatca tcgtatccct tttgaactga gtggtgactt
     1681 aagccgagtg gtaacaagta tcaatggatt ggtggatagt accgtagcag cgattgaaga
     1741 cgaacgtaaa atcgaaaaat caaaagatga attgatcacg aatgtcagtc atgatatccg
     1801 taccccactc acgtccatca ttggatacct aggactgata gaagatggcc agtatcactc
     1861 agaagaagat cttttaaagt atacgcatac tgcttatatc aaagcaaagc agatgaaatc
     1921 tttagtggac gacttatttg aatacactaa ggtacgccag cctgctgtcc cggtgaattt
     1981 ttctgccttt gacatgatcc agttgatcga acagttagca gctgattttg agttggaagc
     2041 ttctaaaaaa aatatccaaa tacttgttca atcaaaagta gactccctta tcatggatgg
     2101 cgataccgaa aaacttgtac gtgtcttcaa taatcttttg acgaatgcgt tgaaatacgg
     2161 aaaaggagca acgaagatcg taatcgaagt agaacgtatc ggttcagaag ttgttgcaac
     2221 agtcaaaaat aacggagcaa tgatcccaca acaggcaatc gataatctat tcgaccgttt
     2281 ttatcgtgta gaagaatctc gttcacaagc aactggagga actggacttg gtttagcaat
     2341 cgctcaaagc attgtggctt tgcacggcgg atacatttat gcaaaatcag ataagcaatg
     2401 gacttctttc atcatgcacc ttccaatcaa gaaaaatgaa aaattaccta ttgatacaca
     2461 ggaagttatt gacgaaagct gaaatattcg ctattcttat tacaattatt agaataaatg
     2521 aaaaacacag aacccagtac actttattct ttttcagaga gctgatggca ggtgtaaatc
     2581 agcaaaggat aatttggaag ttccagttcg tttacgtgcc ctttcgaaat aagaaaggtc
     2641 ggttcaacag ccgttatgtg ttcaacaagt gcagctgacg ctgaacttgg gtggtaccgc
     2701 gaatttcaga ctgattcgtc ccaagaataa tgggatggat cagtcttttt ttattttcaa
     2761 aaaggaggaa atcatatgtt agatgtaaaa atgatgcgac aaaatattga agaagtacaa
     2821 gaaaaattga tgactcgggg cgtaaaaaaa gaaacattag ctgaattcat ggaactagat
     2881 gaaaatcgtc gtaagctact agtcaaaagt gaagaattaa aaaaatatcg aaatgatgtt
     2941 tctgctgaga tcgcccaatt gaagcgtgca aaagaagatg cttctgctaa aatcgctgaa
     3001 atgaaagaag tcggtggcaa catcaaagca ttggatactg aaattgctga agtggatgaa
     3061 aaattgaaac aaattgcaac tactttgcca aaccttcccc atgattctgt gccagttgga
     3121 acagatgaag aggacaatgt agaaatccgt cgttggagcg aaccacgtac atttgctttt
     3181 gaaccaaaac cacactggga aattgcagaa aatcttgata ttttagattt tgaacgtggc
     3241 gcaaaagtag ccggtagccg ttttgtctat tacaaaggat taggtgctcg attagaacgt
     3301 gctgtctaca acttcatgct tgatcagcat atttacgaac acggctatac tgaaatgatc
     3361 actccttata tcgtcaacag taaagcgatg tttggtacgg gacaatttcc aaaattcaaa
     3421 gaagatgttt tccaattgga gaatactgat ctaacattaa tcccaacagc tgaagtacca
     3481 ttaactaact attataatga tgaaatttta gatggtgcag atttacctat ttatttcact
     3541 gcactaagcc ctgcttttcg ttctgaagcc ggaagtgctg gtcgggatac acgaggactg
     3601 attcgtcttc accaattcaa taaagtagaa atggtcaaat tttcggatgt agagcattct
     3661 tacgaagaat tggaaaaaat gacagctgat gcagaagata tcttgaaaaa attgcatttg
     3721 ccttatcgtg tgatggcctt atcaactgga gacatgggct tttctgcagc taaaacttat
     3781 gacttagaag tatggatacc tgcacaagat acttatcgtg aaatcagttc ttgttctaac
     3841 tgtgaagatt tccaagcacg ccgcgcaatg atccgttatc gcgataatga tggaaaaatc
     3901 cactatactc atactctgaa tggttcagga cttgcagttg gccgtacagt agctgccatt
     3961 ttggaaaact accaaaatga agatggttct gtcacagttc cagaagcgct cgttccttat
     4021 atggggaatc tgaaagttat taaataaaga ggctgtgaaa aagccgctta gtttcgagta
     4081 ttaagaaaaa ttttgtgaaa atggctttcc acatttttgc ataaattgga cttaataccg
     4141 agaaactggc ttttgaacac catttatcga gaggttgtga aatcagcgaa ttaacctgag
     4201 tattagagaa aaaaatggaa aaatagtccc tcatatttta ccgtttgttg aactaatacc
     4261 gagggtcagc tgattgaaca ccgtttagtg gagaggtcgt aagcctaccc cccagctcct
     4321 agttacaagg acaaattcaa aaaacagctc ttcatatttt atgaatgttg tacttgtgat
     4381 tcggaggtgg caagggaaca ctgtttattc aaaaagaggc tgggacagaa gcgtttaact
     4441 ccaagaaata agaagaaatt cacgaaaatt gcttttcaaa tttttgtgaa tttcttctta
     4501 tttccgaagg agttgcttct acttccgccg tttatacgtg tttagagcgt gagacaaaag
     4561 tgttctttac ttttgtccca cgctctttaa tgtttttaag attcaactga atagtttggt
     4621 gcttcttttg tgatttgtac atcatgaggg tgtgattctt ttaatccact gccgctcatt
     4681 tggataaatt gcgcttcatc acgtaattgc tgtagattgg ctgccccaac ataacccatt
     4741 cctgaacgca aaccgccgat catttggaaa ataatatctg caacacttcc tttataagca
     4801 acccgacctt cgattccttc tggtactaat ttatttgctt cattgacact accttggaag
     4861 taacggtctt ttgaaccttt ttccatcgcg cctaatgagc ccatacctcg gtatgttttg
     4921 aatcgacgtc cttggaagat ttcgaattca cctggtgatt catctgtccc tgccaacata
     4981 cttcctagca tcactgcatg tccgccggca gctaatgctt tgacgatgtc accagaatat
     5041 ttgatccctc cgtctgcaat aatcgctttg ccatattgac gagcaactga tgctgcatca
     5101 taaatagctg tgagctgagg tacacctaca ccggctacca cacgtgtcgt acaaatcgat
     5161 cctggtccga taccaacttt tacgacatct acacctgcat catataaagc ttttgtcgct
     5221 tcagccgtcg caacgtttcc tgcaatcaat gttgcatctg caaaagttga acggatttct
     5281 tgaatttttc ggatcacacc tgcactatgt ccatgagctg tatcgatgat gattgcatct
     5341 gcccctgctt ctaataaagc attcgcacgc tcaaatgtat cgcttgttac accaacggct
     5401 gctgctacaa gcaatcgacc atgttcatct tttgctgcat ttggaaattc gatcactttc
     5461 tcgatgtctt tgatcgtgat caatccgctt aaacgacctt cgttatctac gattggtaat
     5521 ttttcgattt tatgtttttg caaaatcttt tctgcatctt ttagagaggt acctactggg
     5581 gcagtcacta agttgtcttt tgtcatcaca tcagcaatcg gcatgtgata atctgttaca
     5641 aaacgcatgt cgcggtttgt aattatccct accaattttc tgttttcaag tgtttcaacg
     5701 attggcacac cactgatacg atatttgctc atcaattctt ctgcgtctgc tacaagatgc
     5761 tgcggcgtta agaagaacgg atcaatgatt acgccacttt cagaacgttt gacttttctt
     5821 acttcatccg cttgctggct gatcgtcatg tttttatgga tcacacctag tccgccttgt
     5881 cttgccatag caatggccat cttgctgtcg gtaacagtat ccatacttgc actgatgatt
     5941 ggaatattca acttaatatt tttagctaat tgtacgctca tatccacttc gtttggtaac
     6001 acgtgacttt ctgctggtat caataagaca tcatcaaaag taaatccttt tttcgtgaac
     6061 tttgtttccc agttggacat ttttagtacc actcctctaa tttagtttat ctattaaaat
     6121 aattgatttt ttcatttaag tcaatgatct acttggaatc aagttagctt tctcatagaa
     6181 taccaatata aagcgtttac atctgtgtgt attaaaagag attgtaaaaa agctgtcctg
     6241 catcaagtaa taagaacagc caatcaaaaa cagcttctca tgtttttgaa ttggttgggt
     6301 ttattaccgc agatgcaaac ttttgaacac cgtttagtgg agaggctgtg aaaaagctgc
     6361 ttagtttcga gtagtaagaa aaattttgtg aaaatggctt tccacatttt tgcataaatt
     6421 ggacttaata ccgagaaact ggcttttgaa caccgtttat cgtaaaaagg gactgtgaca
     6481 agacttttgt cacagtccct tactattatt taactatgga taaacaaaat gaggttcttt
     6541 actaccccta gatatctagc aaaatagctg atctttttgc tagtttatct tcttgggacg
     6601 gataaacttc caataatccc gtattttttc ttcaagtagt aacggattgc taaagcagtt
     6661 gccccgataa tgataactac tactggatct aatactggat tgataactgg tggcaatagt
     6721 gttgtcgcag aaaacagtac gacccaaaga agtgtcgttc cacctaggat aagcaatgtc
     6781 ttacccattc ctggtttctt gcttcgatct gctccgggat attcatattg atagatgtat
     6841 ttgtacatca tataaaagac ccagccaccg attgcagaac ctagaacgag tgtagaaaag
     6901 ccataagctg gcgttgttcc acgtgaaaac aatccggtaa tcgcaatcat gatacttaat
     6961 agaccaagca acagcaacgt attatctaac cacatcaata aaggggtgga tgaatttttt
     7021 acttctgctg gcttattgat gattgcttcc gttctttcag agactgtccc aaacaattga
     7081 cgggctgttc ttccgccttt ttgttctgca actaaagtag gcagaatgtc atgaagggct
     7141 tttacttttt cttcttcaga caaattcgct gcttctaaag attttttcaa atcaaaaatg
     7201 tactgttgat ttcttttggt taattttgtt tctaaactat tgttttcttt tacgattgct
     7261 cgtaattgtt ctggttccat tatctaatag tcctccttag ccgttccttt tctcaacggt
     7321 cgtcacaaaa gacattctac catattctac agctagtttg atagcccttt tacacattaa
     7381 agcggaataa cattacgtcg ccgtcttgta cgacataatc ttttccttct aatcgaacac
     7441 gtccagcttc ttttgctgca tgcatgtttc cgtaatgatc caagtcttca aaagagacag
     7501 tttctgcacg gataaatccg cgttcgaaat ctgaatggat gattcctgct gcttgtggtg
     7561 ctttgattcc ttttttgaat gtccaagcac gtacttcttg ttccccagca gtaaagtaag
     7621 tagctaagcc taacaaatca taagcagcac ggatcaactg atccaaacct gattcttcga
     7681 ttcctaaagc ttctaaaaat tccgctttgt cttcttcatc taattctgcg atttcttctt
     7741 ctgcacgagc actgaccacg atcacttctg cattttcttc tgcagcaaag ttgcgcactt
     7801 cttgtacata atgattcgcg tctgcatctg ctacgtcatc ttctgagaca ttggctacat
     7861 aaagaattgg ttttgtcgtc aataaaaata gtgatttaac gattttttgt tcttcttcag
     7921 taaattcgat cgttcgggca gattttcctt cttccagtac aggtttgatt ttatccagta
     7981 cagctagttc tgccactgca tctttatctt tggttttagc aacttttgct acacgagtat
     8041 agcgtttgtt gatagattct aagtctgcta acactagttc caagttgatc gtatcaatat
     8101 cttctagtgg atcaacacgt ccttctacgt gagtaatatt atcatcatca aaacagcgaa
     8161 caacgtgaca aatagcgtct acttggcgga tatggcttaa gaattgattt cccaaacctt
     8221 ctcctttact tgcgcctttt acgattccag caatatctgt aaattcaaaa gtcgtcggaa
     8281 cagttttttt cggatgtaca agttctgtta agcgttgcaa acgccagtca ggtacttcta
     8341 ccatccctac gttggggtcg atcgtcgcaa agggatagtt tgctgcttct gctcctgctt
     8401 ttgtgatcgc attaaacaaa gttgattttc ctacgttggg caacccaaca attccagcag
     8461 tcaatgccat aaatttattc actctttctc tgcatttttt tcaattactt tttttaattt
     8521 cttttcaaaa tctcgacggg tcatcatgac gatatgtccg caattcatac atctgatttt
     8581 gatatctgcg cccattcgaa tgacttccca gcgattcgtc tggcaagcgt gaggtttttt
     8641 catttcaacg atatcgtgca agtcgtacat aaaaatagcc tcctttttgt ttattcttca
     8701 tcaaattgaa tattcaagat atcaagaatt cgagtcaaat ctttttgaga aagatattca
     8761 atctcgatct tacctttccc atttttttct tggatagcta cacttgtacc aaatttatcc
     8821 atcaaacgat cttcgctttc tcggatataa tatggttttt cctttgctag ccttgggatt
     8881 tttttctttt cttttccatc attttgattt aactcagaaa caagctgttc cagttggcgg
     8941 acagttaaat tttcagttac tgcacgattg gctaatttca agatcaatgt tttatttttt
     9001 aaacctaaca gcgtacgggc ttgtcccata gataaacgct gatcttgtac catttccttg
     9061 acaagatccg gtaaagaaag caagcgcaaa taattggcga tataaggtct gctctttcct
     9121 aaacgactag ctacttctgc ttgtgttagt ttcaggtttt tcatcagcat ctcatatgct
     9181 tctgcttctt ctaacggatt cagatcttct cgctgcaagt tttccagcac tgctacttgc
     9241 atcatcgctt cttcgtcaaa gtctctaacg atagcaggga tcgtttcttt ttcagctagt
     9301 ttggacgctc gaaaacgtcg ctcccctgca ataatctcat atccttttac tgcggaacgg
     9361 cgtacgatga tgggttgaaa gacacctgat tgttcaatag aattagctag ttcctgcaaa
     9421 gatacttctt caaatgtttt acggggctga tacggatttg gtcgcagctc attcaatggt
     9481 agctggacaa cggtatcatc ttttacatcc accgtctcta agttttctaa atcttgaaac
     9541 agtgcatcga ttcctcggcc taatccttta cttttcttca cgacttacca cttcctttgc
     9601 caatgcttgg tagacttcag ctcctcgaga acgtggatcg tagtcgatga tcggttttcc
     9661 atgactaggt gcttctgata aacgaatatt tcttggaatg atcgtttcgt aaactttttc
     9721 gcggaagtat ttacgtactt cttctacgac ttcatttccc agatttgttc gtgcatcata
     9781 cattgtcagt aagactccct cgatctctaa ttctggatta aagtgttttt ggactaaacg
     9841 aaccgtattc agtaattggc ttaatccttc caatgcgtaa tactcacact gaacaggtat
     9901 caaaatcgaa tcacttgctg taaatgaatt gattgtcaga tggccaagag aaggcgggca
     9961 atcgatcaaa atataatcat actgactgct tacttctgct aaagatcctt ttaatcttga
    10021 ttctcttgcc atcattgacg tcaattcgat ctctgctcct gctaattgga gagtcgcagg
    10081 cacaatactc aaattttcgt gttcagtgat caaagttgcc tcatcaattg gcaattcatt
    10141 gactagaaca tcatagatgt ctcttgtcac atcaggtttt ctgatcccga caccactggt
    10201 agcatttcct tgtgcatcca tatccactag caatactttt ttgccaaggc tagcaagaca
    10261 agcacctaga ttcaccgtcg tcgttgtttt gccgacgcca cctttttggt tcgcaacaga
    10321 tattatttgt gccatctctt ctcctcctat ttgattggct gcttattagg cagtccgggc
    10381 tttcttgggt atttctttgg ggttggtttc tttttttgaa taacgatgat atgccgttga
    10441 tcagagacat tcggcaaggt aaccgcctct tctttgagga atttaccacc taataaagca
    10501 atcgcttgtt ttgcttctgt caattcctct tcactttttg ctgctttcaa tgcataaaaa
    10561 tagccttctt gtttagccaa gggcaaacac aattccgcta acacattcaa acgagcaacc
    10621 gcacgagcag tgacaaggtc aaatgatgca cgaaagtgag gattttgccc aaatgtttct
    10681 gcccggtcat gatataagga agcatcgatt cctaataatc gggttaactc agataagaag
    10741 gttattcgtt tattcagaga atcaacgatc gttatctgca gttctggaaa ggcaattttt
    10801 aaaggtatgc taggaaaccc tgcccccgaa ccaacatcgc ataaagataa aggttgcgta
    10861 aagtctactt caaatgctaa tgtaagtgaa tcgtaaaaat gcttcagata tacgtctttt
    10921 tcatccgtaa tagctgtaag gttcatcttt tcgttccatt cgactaatgt ccgataataa
    10981 atagcgaatt gattcatttg atgatcggta agctctatcc ctttttctgc caaggattgg
    11041 cgaaattctt ctggtatcat aaatattcct ttcattgtga aacagcaatt gtttcacaga
    11101 ttctctccct actttaacgc aaaatctcta gacatgcaat ccttccatat ggattcttaa
    11161 aaatttgaaa agtaagaaaa gccgatagac tttttctttc tagtatgact cataaaatat
    11221 acacagaaga gagaactagc tttgagcaat ttctgcaaaa actagttctc tcttctattt
    11281 attattacgt attacgctgg ttcactgact ttcgcaatct tcccttgttc gatatagacc
    11341 attaggatac ttaggtctgc agggtttacg ccactgattc ggctagcttg ggcaattgtt
    11401 tctggttgga ttttctgcaa tttttgtttt gcttctgttg ctaatccgtt gattgctgcg
    11461 tagtcgatgt tttctggaat ctttttcgct tccatgcgtt ttaatttttc tactttttcc
    11521 atggcttttt tgatgtagcc ttcatatttg atttggattt ctacttgttc gatttctttt
    11581 cgtgtcaatt cttcattttc tggaataaac cgtagaagtt ctgcataagt aatctcagga
    11641 cgtcttaaaa agtcgcttgc tagtacacca tcttttaatg gtgccgcatt aatcgttgct
    11701 aaaaagtctt ggacttcttc tgttggtttg attcgtacat tcatcaaacg tttgatttct
    11761 tgttcaactg cttctttttt cttcaaataa ttcttgtatt gatcttcttt tactaagcct
    11821 acacggtgac ccatttctgt taatcgcaaa tctgcattat catgacgtaa aagcagacgg
    11881 tattcagcac gtgaagtcag caaacgataa ggttcgttcg ttccttttgt taccaaatca
    11941 tcgatcatga caccgatgta accgtcactt cgtttgatca ctaaaggttc ttttccctga
    12001 actttcaaag ctgcattgat accggcaatc agcccttgtc cagccgcttc ttcataaccg
    12061 cttgtaccat ttgtttgtcc agctgtataa agattttcaa cgactttagt ttctaatgtc
    12121 ggacggagct ggtgtggctc tactacgtca tattcgatag catatcctgt acgcatcatt
    12181 tctacttttt ctaatccagc aatagaatga agcatgttga cttgaacatc ctctggtaaa
    12241 gaagtagata aaccttgaac atagacttct tccgtattta agccttctgg ttccaaaaat
    12301 aattgatgac gtggtttgtc tgcaaaacgc acgatcttat cttcgatgga tggacaatag
    12361 cgagcgccta ccccttcaac gatccctgta aacattgggg cacgatgtaa gttgtctcga
    12421 ataatttggt gagtcgtctc acctgtatag gtcagccagc aagattcctg atctagttta
    12481 taggcactat ctggtgtgct gaagctaaaa tgattcggct ctttgtcacc aggctgctct
    12541 tctgtaacag aatagtcgat cgtacttgat tttactcgag gcggtgttcc tgttttgaac
    12601 cgttcgatag caaagccaag ttcttttaga tgattcgcta gtccaataga aggttgagag
    12661 ttattcggac ctgatgaata tttcaattca ccgataatga tttctccgcg aagtgctgtt
    12721 ccggctgtaa taacaactgc tttggcccgg tagctcgctc ctgtggacgt cactactcca
    12781 tgacaaacac catcttcgac gatcagttct tcgacgattc cttgtcttag cgtcaaattt
    12841 ggttcttttt cgatcgtatg tttcatttct gttgaataag catgtttgtc tgcttgtgct
    12901 cgcaatgctc ggacagctgg gccttttcct gtgttcaaca tacgcatttg gatataggtt
    12961 ttatcgatat tcttacccat ttctccgcct aacgcatcga tttctcgaac gacaacgcct
    13021 ttagctggtc cgcctacaga aggattacat ggcatgaatg ctaccatgtc caaatttatt
    13081 gtcagtaata atgtttttga tcccatacgt gcagcagcaa gtgcagcttc tgaaccggcg
    13141 tgtcctgcgc caacaacgat cacgtcatac gcatctgctt cataatgttc caatcgtttg
    13201 ttcctccttt tattttccta aacaaaattg actgaataat tgagtgatta gctcatcttg
    13261 tacactgtcg ccgacaactt ctcctagata gtcccagcaa cgcgtcatat ctatttgaac
    13321 gagatccaca ggcattcctg aatcaatacc tgcaatgact tcttcaagtg agagggaagc
    13381 tttttctaac aacgcgatat ggcgggtatt agaaacataa gtagcgtctc tttctcctgt
    13441 ttctccgctg aagaataaat cagaaatagc tgattccaaa cgatctagtc cgtcgttttt
    13501 agctacggaa attgaaaaaa ctggttcatt ttcgatcaat ttcttcaatt tttcttgttc
    13561 cagctgtgct ggcaaatctg ttttatttag aagaatgatc cgtttcaatc cacttgtcgc
    13621 ttccaataat tgttcatctt ctgctgtcag tggttcgctt tggtttagaa caagtaagat
    13681 caagtctgct tctgctaatg cttttcggct tctttcgacc ccgatccgtt ccaccacatc
    13741 ttctgtttcc cgaattccag ctgtatcgat tagcttgagc ggtacccccc gaacattgac
    13801 atactcttcg attacatctc gagtcgttcc tgcaatatcc gtaacgattg ctttttcttc
    13861 tcttaacaaa tggtttaaca aacttgattt gccgacattt ggccggccga tgattgccgt
    13921 acttaaccct tcccgcagaa ccttcccttg cttggatgta gcaagtagcg cttggattct
    13981 ttgttggatc atctgcgctt tttcaagcaa taacttcgta gtcaattctt cgacatcatc
    14041 atattctgga taatcaatat ttacttctac ttgtgctaat gtctctaaaa tctcttgtct
    14101 caatgagcgg atcaaagcag acagattgcc gtctaactga ttaagagcta gtcccattgc
    14161 tttgtctgtt tttgcccgaa tcaaatccat cacagcttct gcctgtgaaa ggtcaacacg
    14221 accattcaag aatgctcgtt ttgtgaattc gcctggctca gctaatcttg caccttctct
    14281 taacaatagc tgcaaaattt gattgactac aacgatccca ccgtgacaat tgatttctac
    14341 tacatcttcc cgcgtaaatg tctttggtgc tctcatcacg gagaccatga cttcatcgac
    14401 aagctgattg ctcttgggat caacgatatg tccataatgg atcgtatgac ttggtacttc
    14461 tgataatcgt ttgtttccag attgataaac tttatctgct agttgcacag cttgatcgcc
    14521 acttaaacga acgatactga ttgcaccttc tccaggtggg gtagaaattg ccgcaatcgt
    14581 atcaaattct aaagtaattt cagccatttt ttcacttcct ttttcatttt gcgcacaaaa
    14641 aaagtgccca ctccaccctt tatgcacaat aaacggtgaa cttcgcactt tgactgcttg
    14701 ttcttgatta aattctggtc atagattatc acacttgcta taaggaatca agtgtttaaa
    14761 acaattagat gaagttgagc ctgttctgct tccattacct tatatttaat tgtccttgga
    14821 tgtctctttg cgttctttgg tttattcgaa gagaagatac tgtataaaca aggctttatc
    14881 tttcttaaaa aaaacggtac actaaatggt agcgctatct aaactataca cttttgaaag
    14941 gagaaaatat gtgagcccgt tcagatctgc tgcattggaa gaagaaagtg accctcggat
    15001 caacgatgtc gttttatcta cagggaatat caaccagaaa tatatcgtcc gcgatatcgt
    15061 ttttgttgtg gaatgcttag aagctgattt gtttgaccca tcttttccca aagatgacct
    15121 tttcttaaca acgaagagga aattaaagac gaaggtactt gaatatggtg cgaatgcagt
    15181 aatcaactgt cattttgagt atcagcaaaa aaaacgcgac ggaaagtatt ttctggaact
    15241 ttttgcttat ggaacagtga tccaatttat ccaaacaacg attggttaaa tgaaacagac
    15301 aaaaaaagcg gatcaaaact gaagacagtt tcgatccgct tttttctatc aaagttagtg
    15361 actacttcat cgtatactct gataagaatg atttctagta aaatatactt ggtttgaaaa
    15421 actttgtcaa cgtgatttat tcaaaaagta atggaatcaa taatattttt tagcaggttc
    15481 tactactaaa taacgataag gttcctcacc ttccgaatgt gtttttacat gttcatcttt
    15541 gcttaatgca gcatggattt gtttacgttc aaatgcaggc attggttcta agaaaacagg
    15601 ctgccctgtc cgttttactt tttcagccgt gcgatcagct aaacgctgaa tgatctcttg
    15661 gcgcttttca cggtaattgc ctacattgac tactacagac agcttatttc ttgctaaacg
    15721 atggatgaag acttgtgcca gatattgcaa tgcattcagt gtttttccat gtttcccaat
    15781 cagtatgcct tgcttttgtg tttctaaatg gaaaacaacc atattatttg attctctttc
    15841 cattttgaca agagctggag catcaagctg ttttgaaatt tcagtaagat aaactgccaa
    15901 ttccgttaaa gctgcttcat cacctagctc acttacttct tctgtttttt cttcaagaat
    15961 cgtttcgttg actacctcga tctcagctgt tggttcttct tccaaaattt cttctacagt
    16021 ctgttctact gtttcagaaa ctttttcgct gatacttggc tggatcgaaa gccgtgcatt
    16081 ctttcgaccc attcctagga aacctttttt tccttcttcc agtatttcga tttcgacttg
    16141 gtctttggcc aaacccaaat cagccagacc tagttggaca gcttcgtcta ttgttgcagc
    16201 ttcataaatc ggcattagca ttacctcccc tattatttcc gttttttctt aggattcatt
    16261 gctttcttaa gagcacgttc tctttcgcgg attctccgtg cttcttcttc acgctctttt
    16321 cgaattttga aagggttgtt gatcagcagc gtttgtacaa cttggaaggc attggaaact
    16381 acccagtata atgaaagacc gctggctaaa ttgacaccca tcactaaaat cattacaggc
    16441 atcacgaaat tcatgatctt cattgatgca ttcgattcaa tttgactcat gctagataaa
    16501 tacgtacttg caaaagtaaa gattgctgct aaaatcggca aaataaatgt tggatccgcg
    16561 ttccctaagt tcaaccataa gaaatgccct tgtttcaact ctggtacacg tgaaatagat
    16621 tgccataacg ccatcaagat tggcatttgt actaataaag gaagacaacc agcatatgga
    16681 ttgacgccat tttctgcata taaacgctgc tgctcttctc ttaacttact ttgtgtttcc
    16741 ggatctcttg aactatattg ttgctgcagt gcttttaatt tcggttgtaa ttcttgcgtt
    16801 ttacgcatgc tctttgtttg gaaatgcatc aacggcaata ggatcacgcg gatgatcagt
    16861 gtaaataaaa tgatccctat ccctgcatta ccaaatgaca acgccttgat cgcctcagca
    16921 aagtaataaa caatataacg atcccaaatt cctgtactct gtgcgctgac ttccccagtt
    16981 ccgcaaccgg ataagacgat gaccaaacta acgataccgg ccatcaaaag taaacgtttg
    17041 tatttcttca cttactcttt tcctctctgt cgattatttt tgcaagtttt aagacgtgga
    17101 ctaaattgga gcgaacctca tcataagtca accccttcac acttggtcta gcaatcacaa
    17161 taaaatccaa ctccggatca atttcttctt ttatagaata aactgctgct cggatttttc
    17221 gtttgacttc attgcggttt accgcgttgc cgaccttttt tcctacagat aaaccaactc
    17281 gaaaatgagg ctgtcccggt ttttccaaac ggtaaacgac gaatttgcgg ttagcaaaag
    17341 aatctcccgt atggaacacg gcttgaaact ctcgttcttt ttttactcga tacgcttttc
    17401 tcattagttt tctttccttt tctatctctc acttacgctc ataatcatac cataattacg
    17461 attgtccttt catcatttcc ctaaaaaaga gtataaaaaa aaccactgaa accccagtgg
    17521 cctaagctga aagaactttt cttcctttgc gacgacggct agctaacaca cgacgtccat
    17581 ttttagtact catacgttta cggaatccgt gaactttttg acgtttacgt ttatttggtt
    17641 ggtatgttct tttcatatat tccacctcca aaatgttctt atctatttta taaacacaga
    17701 catactttga tagtataacg acaaagtagt gattttgtca atataaaata gaagatcgat
    17761 tgttaaaact tatccacaag attatttttt tctatgggag tcctatgtgg aaaaagttat
    17821 gcacagggat tatccacaga taataaatgg ctggttttac acagaaaaca cacgtgtaaa
    17881 aaaagtaatc cacaatcagg tgatttcctg tgtattccgt ctcctaacta ttggtatatc
    17941 tatatttttt atccacagat gcaagaggaa atctattgat ttttacgagt tttccacctg
    18001 tgcattagtg gtgtggaaaa cttttttttg acaactgtgt ttttctcttt tttctttttg
    18061 tcgattgtgt ggataattgt
//
LOCUS       DASHHB010000057        17215 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.059, whole genome shotgun sequence.
ACCESSION   DASHHB010000057 DASHHB010000000
VERSION     DASHHB010000057.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.059"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(98..1117)
                     /locus_tag="U2K71_002203"
     CDS             complement(98..1117)
                     /locus_tag="U2K71_002203"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7316235.1"
                     /translation="MAITVKDVAKKAGVATSTVSRVINDHPSISETTKKKVRKIMDEL
                     GYVPNMTARNLGKRISSAIGVILPPLDSKERIGNPFYLEIMEAINEEARNYDMTTAIA
                     TAKNFELLLENVQRMHLQKQVDGFILVYSDSQDTVIDYLYQNHIPFTLIGQPYHHENE
                     IVYVDNDNQLLGKQATEFLIQNGHKNILFVTNTTHESLYFERYFGYQKAMMMAGLPAL
                     SSVVLEHPEDYLSFEKTLKENEATALVVIDDIFALRTMQLAQMYGYVIPETLSVISFN
                     NSIFSTLTHPYLTSIDIDISELGRVAMQKLRELIHEELSSGVRLVIPHRLIKRETVVP
                     LENPY"
     gene            complement(1139..2146)
                     /locus_tag="U2K71_002204"
     CDS             complement(1139..2146)
                     /locus_tag="U2K71_002204"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose mutarotase"
                     /protein_id="HEM7316236.1"
                     /translation="MDNPEISVVPVPETEIKKITLKNDYLEVVLLNYGARLHQIFAPD
                     KEGKSENVLLSYDRFEDVLTDKSFFGATVGPVAGRIRDASWGEHRLEKNCGSHHIHGG
                     TKGWSFQFWEVEVFKTPSSIGVVFYLKDEFSTYPGPITATITYRLTKNELEMTTSASS
                     LVETICNPTNHAYFNLSGNGTRDIYEHQLTVFLDGVLELDQEKLPTGNWTKKEKLPID
                     FRKSPTLQEILACYPDGLDDVFLLHHPRLSKTSLQLFEKHSGRQMTIATSNKSMVLFS
                     TTGFEADFSVNGKQMHSNYGLAIEPQEIPDIVHFPKFGSINLHPGQERISQTIYRFSA
                     Q"
     gene            complement(2139..2804)
                     /gene="pgmB"
                     /locus_tag="U2K71_002205"
     CDS             complement(2139..2804)
                     /gene="pgmB"
                     /locus_tag="U2K71_002205"
                     /EC_number="5.4.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-phosphoglucomutase"
                     /protein_id="HEM7316237.1"
                     /translation="MFKGVLFDLDGVITDTAEFHYRAWRKLGQEIGITIDHTFNEQLK
                     GVSREDSLSLLLAYGGKEHSFSKEEFAELAKRKNDYYLEMIQTIEPKDVFPGVVPLLD
                     SLKEEKIKIALASASKNGPFLLEKMGLTPYFDAIADPAEATNGKPAPDIFLLAAKAVG
                     LKAEECIGIEDAQAGIQAILSSGAQPIGVGRAEDLGEEIPLVPDTTFLTIDYLKEKWH
                     DHG"
     gene            complement(2797..5091)
                     /locus_tag="U2K71_002206"
     CDS             complement(2797..5091)
                     /locus_tag="U2K71_002206"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HEM7316238.1"
                     /translation="MKQIKRLFAIDPWKISTTQLDKKNLRLQESLTSIGNGYMGMRGN
                     FEETYSGDHHQGTYLAGVWYPDKTRVGWWKNGYPEYFGKVINALNFIAIDISINDQLI
                     DLASLEPEDFYWELDMKNGVLSRTFTITTSTNKVRISFERFLSIVKKESAYIYLTIEM
                     LEGSGTVKLVSKLDGNVQNEDSNYDEHFWNEIDRGTIEEIGYLTTKTIPNNFGIEQFT
                     VTAAMRHFVNGKSVIPDYHEDTLALHGCLSAELSQGETWSVEKEIIVLTSRDIPEAQQ
                     AEKAVSELTNQTNNYIQAKEEQTAAWAERWKLADVVIEGDEEAQQGIRFNLFQLFSTY
                     YGEDERLNIGPKGFTGEKYGGATYWDTEAYAVPLYLALAKPEVTKNLLKYRHNQLPQA
                     IHNAQQQGLKGALYPMVTFTGVECHNEWEITFEEIHRNGAIAYAIYNYTNYTGDTEYL
                     KNEGLEVLAEIARFWADRVHFSKRANKYMIHGVTGPNEYENNINNNWYTNTIAAWVLR
                     YTRENYLTYQKEAAITISEEELAKWKDIAENMYFPVDEELGIFVQHDTFLDKDLMPVS
                     DIPTSDLPLNQHWSWDKILRSCFIKQADVLQGIYFFNDTFTKEEKQKNFDFYEPMTVH
                     ESSLSPSIHSILAAELGMESKAVEMYQRTARLDLDNYNNDTEDGLHITSMTGSWLSIV
                     QGFAQMKTAHDQLRFAPFLPKTWKAYSFHINYRGRLLFISVTDEQVTFELLEGSPLEI
                     YVYEQSYSLADRLEITLRKEEANV"
     gene            5351..7516
                     /locus_tag="U2K71_002207"
     CDS             5351..7516
                     /locus_tag="U2K71_002207"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM7316239.1"
                     /translation="MKKIFSFEFWQKFGKALMVVVAVMPAAGLMISIGKSIPLINPDW
                     TPLVTTGGVIENIGWAIIGNLHILFALAIGGSWAKERAGGAFAAGISFILINRITGAI
                     FGVTSDMLANEDAFTHTLFGTKIMVKGFFTSVLEAPALNMGVFVGIIAGFVGAMAYNK
                     YYNYRKLPDALSFFNGKRFVPFVVILWSTIVALILAVVWPNVQAGINNFGLWIAESQE
                     SAPILAPFLFGTLERLLLPFGLHHMLTIPINYTQLGGSYEILSGAQAGTQVFGQDPLW
                     LAWATDLVNLKGAGDMSQYEFVLHNWTPARFKVGQMIGSSGILMGMAFAMYRNVDPDK
                     KARYKSMYFSAALAVFLTGVTEPLEFMFMFAAVPLYVIYSVIQGAAFAMADILPLRVH
                     SFGNIELLTRTPLAIKAGLGGDLINFVLCVIAFGVVTYFLANFLIKKFNFATPGRNGN
                     YDNDSEETVVSNSGTGTADQQVVQIIHLLGGKENIKDVDACMTRLRVSVNDREKVGTE
                     EAWKRAGAMGLIVKDNGVQAVYGPKADVLKSDIEDLLQSGAEIPEPEMVSTGNGQAGS
                     KQYLGIEQELVSAASGEVIPLSEVKDPVFSQKMMGDGFAVIPTSREVVAPIAGKVTSI
                     FPSKHAIGLETKDGIEVLIHMGIDTVQMNQPAFEIAVKEGQEVGTGTKLAEMDLDVIQ
                     NEEKDPTIMIVFTNDKVEEVVIKQLGTVTAGKVIGSIKM"
     gene            7820..8653
                     /locus_tag="U2K71_002208"
     CDS             7820..8653
                     /locus_tag="U2K71_002208"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HEM7316240.1"
                     /translation="MKFMTLNTHSWLEPEPEKKLQELADKILLEDYEVIALQEINQLL
                     ESEEVEPAKLMKFCPVKNQVPIRKDNFAYRLVQLLKEHGKEYFWSWEMSHIGYDKYEE
                     GNALLTKKTLESQVLTVSQFQKKEDYQTRKILIGKTKIDDQDIFVSSCHFSWWTDKKS
                     GFYFEWKNLENYFLETRVPLFFLGDFNNPVDSQGYYTVRESCLLLQDSYVVANEKGKA
                     ATVEKKIDGWEQNTEKLRIDFIFVPEGMQVKKYQRIFDGIDSPIISDHYGVEIELDVN
                     E"
     gene            complement(8755..10455)
                     /locus_tag="U2K71_002209"
     CDS             complement(8755..10455)
                     /locus_tag="U2K71_002209"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316241.1"
                     /translation="MMNLSKVCKGVRQLKKYGPFLAIFFIALAFLMPHLFTHKLILGA
                     DAPFHYNRFYDAAQQIKYGDFHYFPSLYGFQQSGRIVNAVYGPFFAYLQGLIVLLAGS
                     WFHYQILSNFILYLISGFSMLILLRYVNVRNWVSVPVAVLFMSTYSIQYWSINQGFTS
                     WGTCFYPLCMIPIADFVLHKKFPVIKVAVSVGLMTQIHLLSSIMLALMYIPFYLYYFF
                     NQTEKGKALLQLIKSVLLYFVLTANIWAGLAIVYSGNKILPPFVNREMSQKAIDLDGS
                     YWLHYPKAFPILILSGFLLFIFFQQKNTLFQKIVLYTSLVFFFLSTSLIPWTTLIEKN
                     VPFISLIQFPFRFFVPFTLLLLLYLALSLNQWSEQKWFRLLSIFGILIFSAQTIQNLY
                     QHLEKWENETFVSRHTYLFDTPEEARKTFFSKDMSTSLFVFQKTTPDYLPIYKETKDN
                     KYDRYNEEILENETTFIKTHPGGLLTIKWKNTGDKTVHIPVIVYDRTVLQQNGKTLTD
                     YEVTDIGTPIVKQQKGINELTLHYQTPIYFYFILSLTLIGWFTLLCLFIYHRYKLLRA
                     "
     gene            10962..12122
                     /locus_tag="U2K71_002210"
     CDS             10962..12122
                     /locus_tag="U2K71_002210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM7316242.1"
                     /translation="MKQSKQKQSRRLLFRLFLLICIVVGLLIALYPFYVDSLNSFIDQ
                     KRIEQVQKRTAAEIEVQRKKMEEKNQRLTDQGFNPGTDPFNEKNRNESTTSSQLEEWL
                     IGSINIPKIQLNISLYDRLNGRILENGAGVLQGTSFPLGGNSTHSVISAHSGLPNRRL
                     FTELDRLENGDTFILTVLGEKLAYQVKNIQVVLPDDTSVLTISEGEDLVTLLTCTPYM
                     INTHRLLVTGHRIPYNESVKKEEGKGNQERTMRQWFILAGTIIAVVILLVFIGRLIYQ
                     YRLSKKVLDFSFIISDSSGKPVNGSSFILKHKKKTLTRNGVPFSVQSDHHGKVKLDQL
                     PGGTYRIVSADDPKVAVSFGIRKVKQEKIYFFEGRKLVKELQKNGFYFKINE"
     gene            12138..12581
                     /locus_tag="U2K71_002211"
     CDS             12138..12581
                     /locus_tag="U2K71_002211"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7316243.1"
                     /translation="MIRTTTKLSAKELESVLSIWLHANLDSHEFIPSTYWENQVESVR
                     KALPDAVIYLAEIDQEIIGFAGVIDQFIAGIFVKREYRQQRIGTQLMKKIKETNKTLT
                     LTVYQKNQSAIHFYLSQGFTIQTQQKDEAVNEEELVMKWNQNADF"
     gene            complement(13064..13594)
                     /locus_tag="U2K71_002212"
     CDS             complement(13064..13594)
                     /locus_tag="U2K71_002212"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phenolic acid decarboxylase"
                     /protein_id="HEM7316244.1"
                     /translation="MKEFKELEDFIGTHFIYTYDNGWEYEWYAKNDHTVDYRIHGGMV
                     KGRWVKDQEADIVKLTDGVFKITWTEPTGTDVALDFMPNEKKLHGMIFFPKWVEEHPE
                     ITVTFQNEHIAEMEAAREKYETYPKLLVPEFATITYMGDAGIDNEDVISEEPYDGMPD
                     DIRSGKYFDENYKRVK"
     gene            13699..14250
                     /locus_tag="U2K71_002213"
     CDS             13699..14250
                     /locus_tag="U2K71_002213"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM7316245.1"
                     /translation="MARKNTLRYILLGLLSKKKMTGYELNQSFKNEIGEFWQAKHSQI
                     YPELAKMEEQGIIQHQVEITGEKLEKKVYELTEEGKEQLAEWIHTPTNELPVNRDEFV
                     LKLYFVKDVNDPALVEIIQQQRDLHEEKRLHLLERKKTIFPTEKEKHENYGHYLILAH
                     AINRETEYANWLTDVLEESNRKK"
     gene            complement(14403..14876)
                     /locus_tag="U2K71_002214"
     CDS             complement(14403..14876)
                     /locus_tag="U2K71_002214"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM7316246.1"
                     /translation="MVGASLKRLLENDQLVVGISELSDIVGVSPRQLRYWEQKGFIRS
                     IEADANCPRKYRLPTVIKVELIKKYLDDGFTLSKAAEKAEIRQKKMHHVRKVFSKAIK
                     DIELIDERYTILQIGNFKEENAKMYIIHDNETDELTYKLLPVDEKADYEQLLKEL"
     gene            15014..16486
                     /locus_tag="U2K71_002215"
     CDS             15014..16486
                     /locus_tag="U2K71_002215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008269079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEM7316247.1"
                     /translation="MSNNQPVDIYGKPYNRTLLVVVLLIGTFCTVLNQTLLTTAFPTL
                     MKAFDISASDVQWLTTGFLLVNGIMIPITAWLINKFSSRNLYLSAMTIFLIGTITCFT
                     APNFSTLLIGRLIQACGVGVSMPLLQNIMLSIFPPEKRGSAMGMSGIVIGLAPALGPT
                     LSGYIIDNYHWRDLFGMVLPIVVLVLVLAFFLMKSVIPLSNPSIDILSAILSTIGFGS
                     LLYGFSSVGDDGWGSLKVIGFLVVGVIFIALFSWRQLHLEHPFLELRVFKSSTFTIAA
                     VLAGVTNMAMVGAEMVVPLYIQNIRGESAFHSGLMLLPGALIMGAMMPVTGAIFDKYG
                     AKRLAITGMFILTAATAPFAFLTKTTPVLYIVILYAIRMFGISMVMMPVTTSGMNALP
                     NNLISHGTAVNNTFRQIASSIGTAILISVLTNVTKANIPSKSVLDNTPLAYKDQAINA
                     TLSGYHAAFIVAIIFGILGFIIAFLLKKKEPVLMKAGDNA"
     gene            16483..17193
                     /locus_tag="U2K71_002216"
     CDS             16483..17193
                     /locus_tag="U2K71_002216"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4811 domain-containing protein"
                     /protein_id="HEM7316248.1"
                     /translation="MIIGVLIVSLLAFALTNVFAKKTWQTFLSLIFGLIFVASLSLIV
                     ANLSNHFGMEKVTETKTEKIVSSADSQGADMLLYKALGNGKEKVYLYRTNEKQEKPKA
                     TGTDNETNKVEKTDGDAEKVTKTTYWEYKNDMYKFWFNIADNNHEYDSRVNTFKIPET
                     WVELSTDQAAKLAELVKKQQSTMESEAKAYVQDGMVKAMTENPKMSKAEQEQRTKALA
                     AEFQQQAFAKLVKEAKGE"
BASE COUNT         5354 a         3093 c         3535 g         5233 t
ORIGIN      
        1 ttttttacag ataaacaatg tgttagaagt agtatcttct tttacataaa aagctgacca
       61 taggataaat ggtcagcgta tttagtgagt cagctattta gtatgggttt tctaaaggaa
      121 cgactgtctc tcttttgatc aagcgatgag ggatgaccaa acgtacgcca ctgctcaatt
      181 cttcatggat caattcgcgc agcttttgca tagcgacacg tcctaattcg gagatatcga
      241 tatcgatact tgtaaggtaa gggtgtgtta atgttgagaa gatcgagttg ttgaagctaa
      301 tgacggagag tgtctctgga atgacatagc catacatttg cgccaactgc atcgtgcgaa
      361 gggcgaagat atcgtcaatg accaccaatg ctgtggcttc gttttctttc aacgttttct
      421 caaaggacag ataatcctcc ggatgctcta gtactacact ggataacgca ggaagaccag
      481 ccatcatcat tgctttttga taaccgaaat atcgttcgaa atacaaactt tcatgagtcg
      541 tgtttgtcac aaacaggata ttcttatgtc cattctggat caaaaattct gttgcttgtt
      601 tacctagaag ctggttatca ttatctacat aaacgatttc gttctcatga tgataaggct
      661 gtccgatcaa agtgaaaggg atgtggtttt ggtataaata atcaatgaca gtatcttgac
      721 tatctgaata cactagaata aaaccatcta cttgtttttg taaatgcata cgctggacgt
      781 tctctaacag caattcaaaa tttttagcag tagcgattgc tgtagtcata tcataattcc
      841 gcgcttcttc gttgattgct tccatgatct caagataaaa tgggttaccg atccgctctt
      901 ttgaatcaag agggggtaag attacgccga tggcactgga aatgcgtttt cctagattgc
      961 gggcagtcat gttaggaaca taacctagtt catccataat ttttcggact ttcttctttg
     1021 ttgtctcgga aatactagga tgatcgttga tgacacgaga gacggtcgag gtggcaacac
     1081 cggctttttt cgccacatct tttacggtaa tagccatgat aaaaacacct ttctcttgtt
     1141 attgtgcaga gaaccgataa atcgtctgac tgatccgttc ttgaccagga tggaggttga
     1201 tcgatccaaa tttggggaaa tgaacgatat cagggatttc ttgcggttcg attgctaatc
     1261 cgtagtttga atgcatctgt tttccattga cagagaaatc agcttcgaat ccagtcgtag
     1321 aaaataatac catactttta ttagaagttg ctatagtcat ttggcgacca gaatgcttct
     1381 cgaataattg taaggaagtt ttggaaagcc taggatgatg caaaagaaat acatcatcta
     1441 gtccatctgg ataacatgca aggatctctt gcagtgttgg ggattttcta aagtcgattg
     1501 gcagtttttc tttttttgtc caatttccag taggcagttt ttcttgatct aattcaagca
     1561 caccatctaa aaagaccgtt aactgatgtt catagatgtc tcttgttcca tttcctgaaa
     1621 ggttgaagta ggcatggttg gtcggattgc agatggtttc aacaaggcta ctagcagagg
     1681 ttgtcatctc taattcgttt tttgttaaac ggtaagtgat cgtagcagtg attggtcctg
     1741 gataggtaga aaattcatct tttaagtaaa agaccacacc tattgatgat ggagttttga
     1801 aaacttctac ttcccaaaat tggaaagacc agcctttcgt gccaccgtgg atatggtgtg
     1861 atccgcagtt tttctccaat ctatgctcac cccaagaagc atcccggatc cttccggcta
     1921 caggtccgac agttgctcca aaaaagcttt tgtctgttag aacatcttcg aaacgatcgt
     1981 aagacaataa aacattttct gattttcctt ctttgtctgg tgcgaagatt tggtgcaggc
     2041 gggcaccgta attgagaaga acgacttcta agtagtcgtt cttcaatgtg attttcttta
     2101 tttcagtctc tggaactggt acgacggaga tctctggatt atccatggtc atgccacttt
     2161 tctttcaagt aatcgatcgt aagaaaagtc gtatcaggga caagcgggat ctcctctcca
     2221 aggtcttcag cacgtcccac gccaattggt tgagcgccac ttgagaggat ggcttggata
     2281 ccggcttgcg cgtcttcgat cccgatacat tcttctgctt ttagtccgac agcttttgct
     2341 gcaaggagga agatatctgg cgcaggtttt ccgtttgttg cttctgccgg gtcggcaata
     2401 gcatcaaagt aaggtgtaag acccattttt tcaagtagga atggcccgtt tttacttgca
     2461 gaagcaagag caatcttgat tttttcttct tttaaagaat ccagtaaagg cacgaccccg
     2521 gggaagacat cttttggttc gatcgtctgg atcatctcta aataataatc gttttttctt
     2581 ttcgctaatt ctgcaaattc ttcctttgaa aaagagtgtt cttttccgcc ataagctaaa
     2641 agcaaagaga gtgagtcttc tcggctgacg ccttttagct gttcgttgaa tgtgtggtcg
     2701 atcgtaatgc cgatttcctg accaagcttt cgccaagcac gataatggaa ttcagcagta
     2761 tcggtaatga caccgtctaa atcaaacaat acccctttaa acatttgctt cctcctttct
     2821 tagcgttatt tctagacgat ccgctaaaga ataagactgt tcatagacgt aaatttctag
     2881 aggagaacct tctaataact cgaaagtaac ttgttcatct gtgactgaga tgaacaataa
     2941 acgtccgcga taattgatat ggaaagaata agctttccaa gtttttggca gaaatggtgc
     3001 gaatctcaac tgatcatggg cagttttcat ttgagcaaat ccttggacaa tcgacaacca
     3061 gctaccagtc atggaagtga tgtgcaagcc gtcttctgtg tcgttattgt aattatccaa
     3121 atccaagcga gctgttcgct ggtacatttc tacagctttg ctttccattc ctaattctgc
     3181 tgctaaaatc gaatggatac ttggcgaaag ggaagactca tggacagtca ttggttcata
     3241 gaaatcaaag tttttttgct tttcttcttt tgtaaaagta tcattgaaga aataaatccc
     3301 ctgtagtaca tctgcctgct tgatgaagca agatctaagg attttgtccc aagaccaatg
     3361 ctgattcaga ggaagatcgc ttgtgggaat atcggataca ggcatgagat ctttatctaa
     3421 gaatgtgtca tgctggacaa agattcctaa ttcttcgtca actgggaagt acatgttctc
     3481 tgcgatatct ttccatttgg ctagttcttc ttcgctgata gtgatagcag cttctttttg
     3541 ataagttaaa taattttctc gcgtgtaacg caagacccaa gctgcgatcg tatttgtata
     3601 ccaattgtta ttgatattgt tctcatattc gtttgggccg gtcacaccgt ggatcatgta
     3661 tttatttgct cgttttgaaa aatggacacg gtctgcccag aagcgtgcga tttctgctaa
     3721 aacttccagt ccttcatttt tcaaatattc cgtatcgccg gtgtaattcg tataattgta
     3781 gattgcatag gcaatcgcgc catttcggtg aatctcctcg aatgtgattt cccactcgtt
     3841 atggcattcg acaccagtga aggtgaccat aggataaagc gcgcctttta atccttgttg
     3901 ctgagcgttg tggattgctt gcggcagttg attatgccga tatttcagca gattcttagt
     3961 tacttcaggt ttggctaaag caagataaag agggacagca tacgcttctg tatcccagta
     4021 agtggctccg ccgtattttt ctcctgtgaa tcctttgggg ccgatattca atcgttcatc
     4081 ttcgccgtag taagtagaga atagttggaa caaattgaat cgaatacctt gttgtgcttc
     4141 ttcgtctcct tcgatgacca catcggcaag cttccatcgt tctgcccaag ccgctgtttg
     4201 ctcttctttt gcttggatat aattgtttgt ctggttcgtt aattctgaga cagctttttc
     4261 tgcttgctgc gcttctggaa tatctctact agtaagaacg ataatttctt tttccactga
     4321 ccatgtctca ccttgagata attctgcaga caagcaacca tgtaatgcta aagtatcttc
     4381 gtgatagtca gggatgactg attttccatt aacgaaatgg cgcattgctg cagtaacagt
     4441 aaattgttca atcccaaagt tgtttggaat agttttagtc gtcaaatagc cgatttcttc
     4501 tatagttcca cgatcaatct cattccaaaa atgttcatca tagttgctgt cttcgttttg
     4561 aacatttcca tccaattttg atacgagttt gactgttccg ctaccttcaa gcatttctat
     4621 agttaaatag atataagctg attctttttt gacaatgctc agaaaacgtt cgaaggaaat
     4681 gcgtacttta ttagtagaag tagtgattgt gaatgtacga gataatacac catttttcat
     4741 atctaattcc cagtaaaagt cttctggttc caaagaagcc aagtcgatca gctgatcgtt
     4801 gatcgaaata tctatcgcga taaagttcaa ggcgttgatc acttttccaa agtattctgg
     4861 ataaccattt ttccaccagc cgacacgtgt cttatcggga taccagacac ccgccaaata
     4921 agtaccttga tggtggtccc ctgagtaagt ttcttcaaag ttcccgcgca tccccatata
     4981 accgtttcca atacttgtta aagattcttg cagtcgaaga ttttttttat ctaattgtgt
     5041 cgtgctgatt ttccatggat caatagcaaa tagtcgtttg atctgtttca tttttctcct
     5101 cctaatgagc tgttttatac gttcataata aacgcaaccg attgcacaag tcaatagaca
     5161 aaagaaaata tttcatagaa aaaagcttaa aaaaataaat ttttaggaga aaattataga
     5221 agggaacaca cgtattatag gttttataga atggtataga cagaaaaata aaaaataaag
     5281 catttacaaa aaagaaaacg cacgctataa tacgtttaac gaaaccgatt gcgtaaagga
     5341 gctaaacgga atgaaaaaga tatttagttt tgaattttgg caaaaatttg gaaaggcttt
     5401 aatggttgtg gtagcagtta tgccggcagc tgggttgatg atcagtatag ggaaatcaat
     5461 tcccttgatc aatcctgatt ggacgccgct tgtcacaacg ggcggagtaa ttgaaaatat
     5521 tggttgggca attattggta atcttcatat tttatttgct ttagcaatag gaggaagttg
     5581 ggcaaaagaa agagcaggcg gagcatttgc agctgggatc tcatttattt taatcaatcg
     5641 gatcactggg gctatctttg gtgtcacaag tgacatgctt gcgaatgaag atgcatttac
     5701 ccacactctg ttcggtacga agatcatggt taaaggattt tttacaagtg tattggaagc
     5761 accagctttg aatatgggag tattcgtagg gatcatcgct ggatttgtcg gggcaatggc
     5821 ttataataaa tactataatt atagaaaatt gccggatgct ttgtcatttt tcaatggaaa
     5881 gcgctttgtt ccgtttgttg ttatcttatg gtcaacaatc gtggcattga ttcttgctgt
     5941 ggtgtggcca aacgtacagg ctggaatcaa taattttggt ttatggattg cagaatcaca
     6001 agaaagcgca ccgattctag caccgttttt atttggaaca ttagaacgtt tactattgcc
     6061 atttggattg catcatatgc tgacgattcc tatcaactat acacagcttg gcggatcgta
     6121 tgagatttta tcaggcgcac aggcggggac acaagtattt gggcaagatc cgttatggtt
     6181 ggcttgggcc actgatttag tgaatcttaa aggtgcaggg gatatgtcgc aatatgagtt
     6241 tgtgttgcat aactggacgc cggctcgttt caaagtagga caaatgattg gttcttcagg
     6301 tatcttgatg ggaatggctt ttgcgatgta tcgtaatgtc gatcctgata agaaagcaag
     6361 atataaatca atgtattttt cagcagcgtt ggctgtcttt ctaacaggtg taacagaacc
     6421 attggagttc atgtttatgt ttgctgctgt gccattatat gtcatttatt cagtgatcca
     6481 aggggctgca tttgcgatgg cagatatctt gccattgcgg gtccattcat ttgggaatat
     6541 cgaattgctg actcggacac cgttagctat caaagcgggt ctaggtggag atcttatcaa
     6601 cttcgtgttg tgtgtgatcg cttttggtgt tgttacgtat ttcttagcaa atttcttgat
     6661 caaaaaattc aactttgcta caccgggacg taacggaaat tacgacaatg acagtgaaga
     6721 aaccgttgta tctaacagcg gaacaggaac agcagatcag caagtggtcc agatcattca
     6781 tttgctggga ggaaaagaaa atatcaaaga tgtcgatgct tgtatgactc ggttacgcgt
     6841 aagtgtaaat gatcgagaaa aagtagggac tgaagaagcc tggaaacgag caggggcgat
     6901 gggattgatc gtcaaagaca atggagtcca agcagtttat ggaccaaaag cagatgtctt
     6961 gaaatcagat atcgaagatc tgctgcaatc aggagcagag attccagagc cagaaatggt
     7021 ttctacagga aatggacaag caggtagtaa gcaatactta ggtatagaac aagaactagt
     7081 ttccgcagct tctggagaag tgattccttt atctgaagta aaagatccgg tattctcaca
     7141 aaaaatgatg ggtgacggct ttgctgttat tccaacatca agagaagttg ttgcgccgat
     7201 cgctggaaaa gtgacaagta ttttccctag caaacatgca attgggctgg aaacgaaaga
     7261 tggtatcgaa gttttgatcc atatgggaat cgatacagtc caaatgaatc agccagcttt
     7321 tgagattgct gtcaaagaag gccaagaagt aggaacagga acgaaattag ctgagatgga
     7381 tcttgatgtg atccaaaacg aagaaaaaga tccaacgatc atgatcgtat ttacaaacga
     7441 taaagtagaa gaagtagtga tcaagcaatt aggtacagtg acagctggta aagtgatcgg
     7501 ctccattaaa atgtaaggag agattgtgaa aaagctgtcc tgcatcaagt cataagaaca
     7561 gccaaacaaa aatagctcct cctatttttc gtttggttgg gcttattacc gagaagcagc
     7621 agtttgaata ctgtttagtg gagaggtcgt gaaaaagctg ccctgcacca agtgataaaa
     7681 acagccaaac aaaaaatagc tcctggtcca ttgagcttat tactgaggag ttagcaggag
     7741 aacactattt gctggagagg ttgtgacaaa aattttgtca caaccctttt gtttcgaaat
     7801 gaagacaagg agaaaaccaa tgaaattcat gacactcaac acgcatagct ggcttgagcc
     7861 agaaccagag aaaaaattgc aagaattagc agataagatc ctgttagaag attatgaagt
     7921 gattgcttta caagaaatca accaattact ggaatctgaa gaagtagaac ctgcgaagct
     7981 aatgaagttc tgtccggtaa agaatcaagt acctatccgt aaagataatt ttgcctaccg
     8041 tttagttcag cttttaaagg aacacggaaa agagtatttt tggagttggg aaatgagcca
     8101 catcggttat gataaatatg aagaaggaaa tgcactgctt acgaaaaaaa cattggaaag
     8161 tcaggtcctt accgtctccc aatttcaaaa gaaagaagat tatcagacac gcaagatctt
     8221 gattgggaaa acaaagatag atgatcagga catttttgtc tcgagctgcc acttttcttg
     8281 gtggacagat aaaaaaagtg gcttttattt tgagtggaaa aacttggaaa actacttttt
     8341 ggaaactcgc gtaccacttt ttttcctagg tgatttcaat aatccagtag actctcaagg
     8401 ttattatact gtaagagaaa gttgccttct tcttcaagat tcgtatgttg tcgcgaatga
     8461 aaaaggaaaa gcagctactg tggaaaagaa aatagatggt tgggaacaaa atacagaaaa
     8521 attgcgtata gattttattt tcgtccctga aggaatgcag gttaaaaaat atcaacggat
     8581 ttttgatggg atcgatagtc caatcatcag tgatcactac ggggtggaaa tcgaattaga
     8641 tgtgaacgaa taaaaaatat aacatataac ataaaaaaac tgattaaaat aaaactctcc
     8701 aaacgattat accgtttgga gagttttttg atcggatgta tttatccctt ttgcctaagc
     8761 acgtaaaagc ttgtatctat gataaataaa taaacatagc aaggtaaacc agccaatcaa
     8821 agtaagagaa agaatgaaat agaaatagat tggcgtttga taatgaagcg tgagctcgtt
     8881 gattcctttt tgctgcttca cgattggggt cccgatatct gttacttcat aatcagtcaa
     8941 tgtttttcca ttttgctgta gcacagtccg atcgtagacg ataacaggga tatgaacagt
     9001 tttgtcccca gtgtttttcc acttgattgt caaaagaccg cctggatgtg tttttataaa
     9061 agttgtttcg ttttctagta tttcttcgtt ataccggtcg tatttattgt cttttgtctc
     9121 cttatagatt ggcagatagt caggtgtggt tttttggaaa acgaacaagc tggtagacat
     9181 atctttgctg aagaatgttt tccttgcttc ttccggcgta tcgaatagat aagtatgtcg
     9241 actgacaaat gtttcatttt cccacttttc cagatgctgg tataagttct ggatcgtctg
     9301 tgctgaaaaa ataagaatgc caaatatact taaaagacgg aaccactttt gttcagacca
     9361 ctggttcagt gaaagggcaa ggtaaagtag aagcaatagc gtaaatggca cgaaaaatcg
     9421 aaatggaaac tggatcaaac taataaaagg cacatttttt tcgatcaatg tcgtccatgg
     9481 aatcaagcta gtagaaagaa aaaagaaaac aagacttgtg tacaacacga ttttttggaa
     9541 taaggtgttc ttttgttgaa agaaaataaa aagcaaaaaa cctgataata tcaatatagg
     9601 aaaggcctta gggtaatgga gccaataact gccatccaga tctatcgcct tttgactcat
     9661 ttctctgtta acaaagggag gaagaatctt attcccactg taaacaatag ctaatcctgc
     9721 ccaaatattt gctgtcagaa caaaataaag caagaccgac ttaatcagtt gaagcagtgc
     9781 ttttcctttt tcagtctggt taaaaaagta ataaagataa aaagggatat acatcaaagc
     9841 taacatgatc gaagacagta aatggatctg tgtcataagt cccactgaga cagcgacttt
     9901 gataacaggg aactttttat gaaggacgaa atcagcaata gggatcatac ataaaggata
     9961 aaaacaagtc ccccagctag tgaatccttg gttgatagac cagtattgaa tagaatacgt
    10021 actcatgaat aaaacggcta ctggtacact tacccaattt cgaacattga catatctcag
    10081 taaaatcagc atagaaaatc ctgaaataag ataaagaatg aaatttgaaa gaatctgata
    10141 atgaaaccag ctacctgcaa gcaacacgat caatccttgt aaataagcga aaaatgggcc
    10201 gtaaacggca ttcacgatcc tgccactttg ctggaatcca taaagggaag gaaaataatg
    10261 gaaatcccca tatttaattt gttgcgcggc atcatagaac cgattgtaat ggaatggggc
    10321 atcagcaccc aaaattagtt tatgtgtgaa taaatgaggc atgagaaatg ccaaagcaat
    10381 gaaaaaaatt gctaaaaatg ggccatattt tttcaattgt cgaactcctt tgcaaacttt
    10441 agataaattc atcatatatc ctatgcgaga ggtaggcaag aaattagaaa attcatgaaa
    10501 atatttatga attttacaag aaaaacataa gtgaaagttc taaggagaga ggatgcgaca
    10561 taagttttgt tgcatctcct tgttttgaaa cagaattttt cttaatactc gaaacaaagt
    10621 agctttttca tgacctctcg gtaaacggtg ttcaatcagc tgcttctcgg taataagctc
    10681 actagcctag aaaatatgag aagctatttt ctgactagcg ctcttattac tcaaagcata
    10741 acgcagtttt cacaacctct tccttaaacg gtgttcaaaa gcttgcatct gcggtaataa
    10801 gcccaaccca acaaaaaata tgagtagcta tttttttggg ctgtacttat tacttgatgc
    10861 aggacagctt tttcacaacc tcatcggctt tcattttcta tttttttcct gaacccatat
    10921 aataaaagaa agaacaaaaa aacttgcaag ggaggacaca tgtgaagcag agtaagcaaa
    10981 aacagtctcg tcgacttttg tttcgtttgt ttttattaat atgtatcgtc gtagggttac
    11041 tgatcgcttt atatccattt tatgtggatt ctttaaatag ttttattgat caaaaacgta
    11101 tcgaacaagt acaaaaacgg accgcagctg aaattgaagt gcagcgaaag aaaatggagg
    11161 agaaaaacca acgattaact gaccaaggat tcaatcctgg tactgatcct ttcaatgaaa
    11221 agaacagaaa tgaaagtaca acatccagtc aattagagga atggctgatc ggaagtatca
    11281 atatacctaa gatccaatta aatatttcac tttatgatcg gctcaatggt aggatccttg
    11341 aaaatggagc aggcgtcttg caaggaacct ctttcccgct tggtggaaat tcgacgcatt
    11401 cagtgatctc tgctcacagc ggtttaccta atcgacggtt atttaccgaa ttagatcgtt
    11461 tagaaaacgg agacacgttt attttgactg ttttggggga gaaattggct tatcaagtta
    11521 aaaatataca agtggtactg ccagatgaca cgtctgttct aacgatttca gaaggagaag
    11581 acttagttac cttgcttaca tgtacgccgt atatgatcaa cacgcatcgt ttgctagtga
    11641 caggacatcg gattccttat aacgaatcgg taaaaaaaga agaaggaaaa ggaaatcagg
    11701 aaagaaccat gcggcaatgg tttattcttg caggaacgat aatagctgta gtcatactgt
    11761 tagtgtttat cggacgacta atctatcagt atagattaag taaaaaagtg cttgattttt
    11821 catttattat tagtgattcg tcagggaaac cggtaaatgg tagttcattc attttgaaac
    11881 ataagaaaaa aacacttact agaaatggtg tgccgttttc agtacaaagc gaccatcatg
    11941 ggaaagtcaa gctggatcaa ttgcctggtg gaacttatcg aatcgtttca gctgatgatc
    12001 ctaaagttgc tgtttctttt ggtattagaa aagtaaaaca agagaagata tacttttttg
    12061 aggggcgaaa acttgtaaaa gagcttcaaa aaaatggttt ttattttaaa atcaatgaat
    12121 aagtaaggag agaaaaaatg atccgcacaa caacaaaatt gtctgccaaa gaactggaat
    12181 ctgttctatc gatttggctg catgctaact tggattctca tgaattcatt ccatcaactt
    12241 attgggagaa tcaagtagaa tctgttagga aagctttacc agatgcagtg atttacttag
    12301 cagaaataga tcaagaaatt attggttttg ctggggtgat tgatcagttt atcgcaggaa
    12361 tctttgtgaa aagggaatac cggcaacaaa gaatcggtac gcaactaatg aaaaaaataa
    12421 aagaaaccaa taaaacactg actttgacag tctatcaaaa aaatcaatca gctattcatt
    12481 tctatctttc acaaggtttt acaattcaaa cacagcaaaa agatgaagca gtaaatgagg
    12541 aggaacttgt gatgaaatgg aatcaaaacg ctgatttttg atgaacagaa taacgaaata
    12601 atgctcccag cctaattttt tgctgggagc attatttcat tttgaagtca ctaatttaac
    12661 tgtgtgaata tgcagttgtt acaatctttg ataaacggtg ttcaatcagc tgaccctcgg
    12721 tattagctca aaaaacggca aaatatgagg aactattttt ccatttgttt ctctaatact
    12781 caggtcaaaa cgctgatttc acaacctctt gataaacggt gttcaaacgg ctgcttctcg
    12841 gtaataagcc cactagccta gaaaatatga aaagctattt tctaactagc gttcttatta
    12901 ctcaaagcat aacgctgatt tcacaacctc ttgataaacg gtgttcaatc agctgaccct
    12961 cggtattagc tcaaaaaacg gcaaaacatg aggaactatt tttccgtttc tttctctaat
    13021 actcaggtca gaacgctgat ttcacaacct ctctttcaca accttatttt actcgtttat
    13081 aattttcatc gaagtatttt ccgctacgga tatcatctgg cattccatcg tatggttctt
    13141 cgctaataac atcttcattg tctatacctg catcacccat ataagtgatc gtagcaaatt
    13201 caggaacaag taattttgga taagtttcat atttttcacg agctgcttcc atttctgcga
    13261 tatgttcgtt ttggaaagta acagtgattt ctggatgttc ttcaacccat ttagggaaaa
    13321 agatcattcc gtgaagtttc ttttcatttg gcataaagtc caatgccacg tctgtccctg
    13381 ttggctctgt ccaagtgatt ttgaatacgc cgtccgtcaa tttaacgata tcagcttctt
    13441 ggtcttttac ccaacggcct ttgaccattc cgccatggat acggtaatca acagtatggt
    13501 catttttggc ataccattca tattcccaac cattgtcata cgtatagata aagtgagttc
    13561 caataaaatc ttccaattct ttaaattctt tcatgtttaa gcatctcctt ataaattaaa
    13621 acatgtaata aactacatgt tttcgattac tcatttatga tagaatggat ttaaagattt
    13681 gtcaaggagg gaaaactgat ggcaagaaaa aatacacttc gatatatttt gttagggtta
    13741 ttaagtaaga agaagatgac aggatatgaa ttgaatcaat cgttcaaaaa tgaaatagga
    13801 gaattttggc aagcaaaaca tagtcagatt tatcctgaac ttgcaaaaat ggaagagcaa
    13861 ggaatcattc agcatcaagt agagatcaca ggtgaaaaat tggaaaagaa agtttatgaa
    13921 ttaacagaag aagggaagga acaattggca gagtggattc atacacccac caatgaacta
    13981 ccggttaatc gagacgaatt tgttctgaaa ttgtatttcg tcaaagacgt gaatgatccg
    14041 gcattagtag aaattattca gcagcagcgt gaccttcatg aagaaaaacg acttcatcta
    14101 ttagagcgaa aaaaaacgat ctttcctact gaaaaagaaa aacatgaaaa ttacggacac
    14161 tacctgattt tagcgcatgc gatcaatcgg gaaacagaat atgcaaattg gctaacagat
    14221 gtattagaag aaagtaaccg aaaaaaataa aaaaatatcc gaacagtgat ggagaattcc
    14281 tttctatatg aaagaaacgc agatcaccgt tcggatattc ttatgttcaa acaggccttt
    14341 tttcaagtat ttatgtagca tctacttatt aaaaagcttg gtacttagga gattagtggc
    14401 gtttagagtt cttttaacaa ctgttcgtag tcagcttttt catcgacagg aaggagcttg
    14461 taagttaatt cgtctgtctc gttgtcatga atgatgtaca tcttagcatt ctcttctttg
    14521 aagttaccga tttgtaaaat cgtatagcgt tcgtcgatca attcgatatc tttgattgct
    14581 tttgaaaaaa ctttacggac atggtgcatt ttcttttggc ggatttctgc cttttctgca
    14641 gcttttgaca aagtaaatcc gtcatctaaa tattttttga tcagttctac ttttatgact
    14701 gtaggtaaac gatattttct aggacaattc gcatcagctt cgatcgaacg aatgaatccc
    14761 ttttgttccc aataccttaa ttgacgtgga gacacaccta cgatatcact taattcactg
    14821 atgccaacaa ccaattgatc attttccaac aaacgtttaa gggaagcccc aaccattttt
    14881 gtcactcctt ttgtcctctt gcttccctta attatatccg tattaactta tgtgtcaagt
    14941 aggttgacaa atagcgaatt taggactaaa gtattctagt agtttcagaa aaaattcaga
    15001 aagaaggaga aatatgagta acaaccaacc cgtagatatt tacggaaaac catataatcg
    15061 gacactttta gttgttgtct tattaattgg tacattttgt accgttttga atcagacctt
    15121 attgacgaca gcttttccta cattgatgaa agctttcgat atttcagcgt ctgatgtgca
    15181 atggttaacg actggtttct tgctagtcaa cgggatcatg atcccaataa cagcatggct
    15241 gatcaataaa ttcagttcaa gaaatttata tctttctgcc atgacgatct ttttgatcgg
    15301 aacgattact tgctttacag cacctaattt tagtacatta ctaatcggtc gtttgatcca
    15361 agcttgcggt gtgggagtat cgatgccatt attgcaaaat atcatgctat ctattttccc
    15421 gccagaaaaa agaggatcag caatggggat gtcagggatc gttattggac ttgctcctgc
    15481 tttaggccct acattatctg gttatatcat tgataactac cattggcgtg acttgtttgg
    15541 tatggtactg ccaatcgttg ttcttgtctt ggttttagca ttcttcctaa tgaaaagtgt
    15601 tattccattg tcaaatccta gcattgatat tttatccgct attttatcaa cgattggatt
    15661 cggtagttta ctttacggat tttctagtgt aggagatgac ggctggggaa gcttgaaagt
    15721 aattggtttc ttagttgttg gtgtgatttt catcgccttg ttctcatggc gtcaattaca
    15781 tttggaacat ccattcttgg aacttcgtgt attcaaatca tctacattca cgattgcagc
    15841 tgttttagct ggtgtaacaa atatggcgat ggtaggggca gaaatggtcg taccattgta
    15901 tattcaaaat attcgtggag aatcagcatt ccattctggc ttgatgttgt tgccgggtgc
    15961 attgatcatg ggggctatga tgcctgtgac gggtgcgatt ttcgataaat acggcgcgaa
    16021 acgtttagcc atcacaggga tgttcatttt gacagcggca actgcaccat ttgctttctt
    16081 gacaaaaacg actccagttt tatatatcgt gatcctttat gcgatccgta tgtttgggat
    16141 ctccatggtc atgatgccgg taacgacttc tggtatgaat gcattgccaa ataacttgat
    16201 cagtcatggg acagcggtaa ataatacatt ccgtcaaatc gcaagctcga ttggtacagc
    16261 tattttgatc agtgtgttga cgaatgtgac aaaagcaaat atcccaagca aatctgtatt
    16321 ggataatacg ccattagctt ataaagatca ggcaatcaat gccacactgt caggttacca
    16381 tgccgcgttt atcgtagcga tcatctttgg gattcttggc tttattattg cctttttatt
    16441 gaagaaaaaa gaacctgttc taatgaaagc gggggataat gcatgattat tggagttttg
    16501 attgtcagct tgcttgcttt tgctttaacg aacgtgttcg ctaaaaaaac atggcaaacc
    16561 tttttatctt tgattttcgg actaatcttc gtcgcttcac taagtttgat cgtcgctaat
    16621 ttgtccaatc actttggaat ggaaaaagta acggaaacaa aaacagaaaa aattgtttcc
    16681 agtgctgatt cccaaggagc agatatgctt ttgtataaag ctttagggaa tggaaaagaa
    16741 aaagtgtatc tttaccgaac aaatgaaaaa caagaaaaac caaaagcaac tggtacggat
    16801 aacgaaacaa ataaagtaga aaagacagac ggcgatgctg aaaaagtgac gaaaactact
    16861 tattgggaat acaaaaacga catgtataaa ttctggttca atattgctga taacaatcac
    16921 gaatatgaca gccgagtgaa tacattcaaa atcccagaga catgggtaga attaagtacg
    16981 gaccaagcag ccaagttagc tgaattagtg aagaaacagc aaagtacgat ggaaagtgaa
    17041 gcaaaagctt atgttcaaga cggtatggta aaagcaatga ctgaaaatcc aaaaatgagc
    17101 aaagcagagc aagaacaacg tacgaaagcc ttagctgctg aattccaaca acaagcattc
    17161 gcaaaattag tgaaagaagc aaaaggcgaa taatattttg aacaccatgt aggag
//
LOCUS       DASHHB010000058        17007 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.060, whole genome shotgun sequence.
ACCESSION   DASHHB010000058 DASHHB010000000
VERSION     DASHHB010000058.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 17007)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 17007)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..17007
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.060"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..126)
                     /locus_tag="U2K71_002217"
     CDS             complement(<1..126)
                     /locus_tag="U2K71_002217"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EntF family bacteriocin induction factor"
                     /protein_id="HEM7316249.1"
                     /translation="MEEKNRLNAKQCSDQELKKIKGGAGTKPQGKPASNLVECVFS"
     gene            complement(230..541)
                     /locus_tag="U2K71_002218"
     CDS             complement(230..541)
                     /locus_tag="U2K71_002218"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEM7316250.1"
                     /translation="MKKNAKQIVHELYNDISISKDPKYSDILEVLQKVYLKLEKQKYE
                     LDPSPLINRLVNYLYFTAYTNKIRFTEYQEELIRNLSEIGRTAGINGLYRADYGDKSQ
                     F"
     gene            complement(543..740)
                     /locus_tag="U2K71_002219"
     CDS             complement(543..740)
                     /locus_tag="U2K71_002219"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II bacteriocin"
                     /protein_id="HEM7316251.1"
                     /translation="MKHLKILSIKETQLIYGGTTHSGKYYGNGVYCTKNKCTVDWAKA
                     TTCIAGMSIGGFLGGAIPGKC"
     gene            complement(1134..2861)
                     /locus_tag="U2K71_002220"
     CDS             complement(1134..2861)
                     /locus_tag="U2K71_002220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease/substrate binding
                     protein"
                     /protein_id="HEM7316252.1"
                     /translation="MNNLLNYQLPVANWVENITEWFTTTFSGLFSFLQTIGQAVMSGI
                     TNLLLVIPAPLFILLLTVAAFFISKKRPGLTLFTLIGLWFIYNQGLWNDLMNTVTLVL
                     LSSVISIIIGVPLGILMAKSSKAQSIIKPILDFMQTMPGFVYLIPAVAFFGIGMVPGV
                     FASVIFALPPTVRFTNLGIRQVPKELVEASDSFGSTSRQKLFKLELPLAKSTIMAGIN
                     QTTMLSLSMVVIASMIGAPGLGRGVLSALQRAQVGNGFVNGVALVILAIIIDRFTQHL
                     NQPNNKKAAGATTQKSKKRQGLIIGAVVVVILGAIGIGSFSSAKETKRINLSYVEWDT
                     EVASTNVVGEVLKQMGYDVTMTPLDNSIMWKSVSNGESDAMVSAWLPKTHGSQYAQYK
                     DQVEDLGANLTGAKVGLAVPAYMDVNSIDELTDQAGKKIIGIEPGAGVVTAAENTIQK
                     YDNLKDWKVETSSSGAMTVALGQAIKKHEPIVVTGWTPHWMFAKYDLKYLEDPENGMG
                     SEEQIHTMVRKGLKEDQPEAYKVLDNFHWSEKDMEKVMLEINNGKDPQQAAKDWIKEN
                     QELVESWKK"
     gene            complement(2854..4047)
                     /locus_tag="U2K71_002221"
     CDS             complement(2854..4047)
                     /locus_tag="U2K71_002221"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine betaine/L-proline ABC transporter
                     ATP-binding protein"
                     /protein_id="HEM7316253.1"
                     /translation="MVKVEVKHLTKIFGKKTQAALDMMNDHQPKTEILKKTGATVGVY
                     DVNFDVKEGEIFVIMGLSGSGKSTLIRLLNRLIEPTSGSIYIDGEDVSKLSKEELREV
                     RRHKINMVFQNFGLFPHRTILENTEYGLEVRGVPKEERQEKAEKALENSSLLSFKDQY
                     PSQLSGGMQQRVGLARALANDPEILLMDEAFSALDPLIRREMQDELLDLQANVQKTII
                     FITHDLNEALRIGDRIALMKDGEIMQIGTGEEILTNPANDYVREFVEEVDRSKVLTAQ
                     NIMVPALTTNIESDGPNVALTRMRNEEVSMLMAVDRKRHLKGIITADQALEARKQKRP
                     LIDFLDENVTVIGKDMVVSDIFNIIYDSPTPLAVVEDGKLKGVVIRGSVIEALAETSE
                     VSEHE"
     gene            complement(4234..4878)
                     /locus_tag="U2K71_002222"
     CDS             complement(4234..4878)
                     /locus_tag="U2K71_002222"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM7316254.1"
                     /translation="MSGKKKIVRPRYQQIAVDLAERIVENRYKVGEKIHARSTLASTY
                     NASPETTRKAINVLVDLGIMEVRHGSGAFVASKEKAKDFLLQYQDVQSIQDTKAEIMA
                     SVEKQQDELNHFSQLLDQLVNQTKRIHKMNPLLPFELSLSEKAMHLDRSISELNIWQA
                     TGATVIAISHNEELLVSPGPYAKFTAGDIVHFIGNEYTLQRMTNFFYPEHQLAE"
     gene            complement(4972..5106)
                     /locus_tag="U2K71_002223"
     CDS             complement(4972..5106)
                     /locus_tag="U2K71_002223"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoB-associated Cys-rich membrane protein"
                     /protein_id="HEM7316255.1"
                     /translation="MATFLLSVLIFGSAGAIVYTRLKSGKNCDDCQTACPVKKEQIKR
                     "
     gene            complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="U2K71_002224"
     CDS             complement(5108..7240)
                     /gene="feoB"
                     /locus_tag="U2K71_002224"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HEM7316256.1"
                     /translation="MNIALVGNPNSGKTSLFNLLTGSNQYVGNWPGVTVEKKEGSLKK
                     NPNIHIQDLPGIYSLSPYTPEEVIARDYLVQEQPELIINIIDATNLERNLYLTTQLLD
                     LDIPMIIGLNMMDVIKKEGKKINLEKLSYGLGVPVLPISVLKKQGIEKLIEKIQESAK
                     KRPSIPVPCTYDPRLEAALHEIEDVLGEQSGKKRWYAMKYFERDQKVREDYEIAVSQQ
                     KEIEQLIQLTEKLLDDDSETILVNERYEFITQLCTLCVVSNDSFQLSMSDKIDQIATN
                     RWLALPIFAFVMWLIYYLAIQTVGTMGTDWINDTLFGTWLPEHVGRLLAEWQVAGWMQ
                     ELILNGIIAGVGAVLGFLPQLIILFLCLSFLEDCGYMARIAFVMDRLFRKFGLSGKSF
                     IPMLIATGCGVPGVMASRTIENEQDRRLTIMVTTFMPCSAKLPIIALVAGAFFPHQSW
                     VAPSAYFLGMTAIIFSGISLKKTRLFAGDTAPFIMELPAYHFPQWSAVLRQTYDRSKS
                     FVKKAGTIIFVSSIVIWFSSSYNFYAQPVSEDQSILAFFGRILAVVFQPLGWGNWQGT
                     VAAITGLVAKENIIGTFGILFGHAEVSENGREIWQVLQHTYTPAAAYSLLAFNLLCAP
                     CFAAIGAIHREMNAKKWTWIAIGYQCGLAYLVSFVIYQLGHLLEGGQVALGTYLAILL
                     MIGLVYVLFRQPKSKNQFYTILSLEGEE"
     gene            complement(7237..7710)
                     /locus_tag="U2K71_002225"
     CDS             complement(7237..7710)
                     /locus_tag="U2K71_002225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein A"
                     /protein_id="HEM7316257.1"
                     /translation="MQTLAQAKQNFVYQVERIETEEKTKRHLQNIGIVAGSKIVVVNL
                     SGNNAILLVKSHRVAVSREILGQIFVNKKTASSESWTSLDQLKAGETGIVVAIHGQGA
                     VKRRLMDMGLTRNVKIVIRKLAPLGDPIELTVRGYELTLRKSEAELILIQREEKE"
     gene            8111..8335
                     /gene="nrdH"
                     /locus_tag="U2K71_002226"
     CDS             8111..8335
                     /gene="nrdH"
                     /locus_tag="U2K71_002226"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin-like protein NrdH"
                     /protein_id="HEM7316258.1"
                     /translation="MNVKIFSKNNCMQCKMVKRFLTENQIAFEEVNIDEQPEAVDWLK
                     AQGFQSVPVITSDAETVIGFRPDQLRKLAS"
     gene            8494..10653
                     /gene="nrdE"
                     /locus_tag="U2K71_002227"
     CDS             8494..10653
                     /gene="nrdE"
                     /locus_tag="U2K71_002227"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit alpha"
                     /protein_id="HEM7316259.1"
                     /translation="MSLKNLQDVSYFKLNNEINRPVNGQIPLNKDKEALVAFFEENVK
                     PNTMTFPSIMEKINYLIDQEYIEAEFIQLYSPSFIEKLYQFLDEQHFSFKSFMAAYKF
                     YSQYALKTNDGSAYLETYEDRVAFNALYFANGDEELALALADEMIHQRYQPATPSFLN
                     AGRKRRGELVSCFLTQVTDDMNSIGRSINSALQLSRIGGGVGITLSNLREAGAPIKGY
                     EGAASGVVPVMKLFEDSFSYSNQLGQRQGAGVVYLNVFHPDIIAFLSAKKENADEKIR
                     VKTLSLGVIVPDKFYELTRKNEDMYLFSPYSVEKEYGVPFSYVNITAEYDHLVANPNI
                     RKQKIKARDLENEISKLQQESGYPYVINIDTANRENPIDGKIIMSNLCSEILQVQTPS
                     VINGKQEYEVLGTDISCNLGSTNIVNLMQSPNFGQSVRTMTRALTFITDASDIDVVPP
                     IQNGNKLNHTIGLGAMGLHTYLAKEQMEYGSEDSLDFTNIYFMLLNYWTLVESNNIAR
                     ERKQVFHNFEKSAYADGTYFEKYVTGEFQPKSEKVKALFEGIFIPTAEDWNALKQAVM
                     KDGLYHQNRLAVAPNGSISYINDTSASIHPITRMIEERQEKKIGKIYYPAPYLSNETI
                     PYYTSAYDMDMRKVIDVYATAQQHVDQGMSLTLFMRSEIPEGIYEWKTTTKQTTRDLN
                     ILRHYAFHKGIKSIYYVRTFTDDAEEIGSNQCESCVI"
     gene            10703..11668
                     /gene="nrdF"
                     /locus_tag="U2K71_002228"
     CDS             10703..11668
                     /gene="nrdF"
                     /locus_tag="U2K71_002228"
                     /EC_number="1.17.4.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class 1b ribonucleoside-diphosphate reductase
                     subunit beta"
                     /protein_id="HEM7316260.1"
                     /translation="MSETYYEAINWNEIEDIIDKSTWEKLTEQFWLDTRIPLSNDLDD
                     WRTLSQLEKDTVGHVFGGLTLLDTVQSESGMDQLRKDVRTPHEEAVLNNIQFMESVHA
                     KSYSSIFSTLNTKKEIEEIFDWTNTNPYLQKKAERINEIYKHGTPLEKKIASVFLETF
                     LFYSGFYTPLYYLGNNKLANVAEIIKLIIRDESVHGTYIGYKFQLGFNELPEAEQETL
                     KDWMYNLLYELYENEERYTEELYDPIGWTEEVKTFLRYNANKALMNLGMDPLFPDTAN
                     DVNPIVMNGISTGTSNHDFFSQVGNGYLLGHVEAMKDDDYLIGLE"
     gene            complement(11757..12896)
                     /locus_tag="U2K71_002229"
     CDS             complement(11757..12896)
                     /locus_tag="U2K71_002229"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HEM7316261.1"
                     /translation="MQSRFKQSKLFFWTVELVLTIIGVYFILQMPNIFSPILKMLSAI
                     FLPLLVAGFIYYMFDPIVVFLEKHRIPRVFGFLLTFLVVIILVVLTVMNVVPQLMEQT
                     IQLTQSLPMYADETSKWLNELAQREEFKNFNLEEQLASANLSLSNLFNIILVSLSASV
                     SKIISFMMQFFILLFTVPFILLFMFKDGHKFIDALSHFFPKAIRKELRQTVKELNETL
                     SAYISSTILDALIIGVMSFLAMRIFDQPYSLLLAVICGITNIIPYVGPFIGAVPAIIV
                     GLFISPFQALYMALSILVIQQLDGNLIKPLLFGKSMNIHPLTIILVLIGAGSVAGILG
                     MLICIPVYAVIKTIILNIRKIYLLRRMEHVSEKLDSPKVLEETSE"
     gene            complement(13205..13918)
                     /locus_tag="U2K71_002230"
     CDS             complement(13205..13918)
                     /locus_tag="U2K71_002230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HEM7316262.1"
                     /translation="MNDMVLTIPEIILRLGIAMLFGGAIGFERQYKNRPAGMRTHILV
                     CMGACIIALIQVEIASGALRDALEYPQLAGTIRSDEARLIAQVVSGVGFLGAGTIIVT
                     KQSVTGLTTAASLWAIAGLGIAIGMGYYSIAIISFIGIAIALTVVKRVIHVPTTKKLE
                     IQFIHRKETKDFLTDYFEEKNIVIEDVNFDVKFVDDYRVYKNIYTIDLPKGLTYADVI
                     EELSVHKNITKLHLVSIAQ"
     gene            complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="U2K71_002231"
     CDS             complement(14172..14873)
                     /gene="nagB"
                     /locus_tag="U2K71_002231"
                     /EC_number="3.5.99.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosamine-6-phosphate deaminase"
                     /protein_id="HEM7316263.1"
                     /translation="MEIIRVKNAEEGGKKAFEIIKEGMNKGAKVLGLATGSTPVTLYK
                     EMTSSDLDFSEMTSVNLDEYVGLGGSDEQSYRHFMDVQLFDKKPFKETFVPDGKAEDL
                     EAACKKYDQIIDAHPIDIQILGIGQNGHIGFNEPGASFDGTTSVVDLTESTINANKRF
                     FDKAEDVPTKAVSMGIGSIMKSKEIVLMAFGESKADAIKGMIEGPVTNHLPASVLQNH
                     DNVVVIIDEEAASKL"
     gene            15107..16639
                     /locus_tag="U2K71_002232"
     CDS             15107..16639
                     /locus_tag="U2K71_002232"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HEM7316264.1"
                     /translation="MSILTLNNITQQFGDKILYEGVDLQLNAGEHLGLIGQNGAGKST
                     LIKIITGEILPDDGQIHWQKNIHKGYLDQYVEVDETLTIEEFLKTAYAKEYEKEEKIA
                     FLYQTYSETFDDSLLEKAGKLQTELDQGVFYQIDTLVAEMSSGLGIDVLGLDSLLKNL
                     SGGQRSKVILAKLLLENPDVLILDEPTNHLDDQHIQWLTQFLQDFDGAYIVISHDQEF
                     LDQITTHIADIEFGKITKYTGHLKQALKQKEQNRESYLRQYHAQQKQIEKTEAYIRKY
                     KAGSRSTMAKSRQKQLDKIERLTPPASASKAVFDFPFSPIVTTLAVETTDLVIGYDRP
                     LLAPINLTIRFGENIAIRGFNGIGKSTLLKTLIGEIEKISGDFHFPDNTKINYFSQDL
                     YWENPLETPLQYLSSQFPKATIKELRRQLAKAGLVNQLASEPLTTLSGGEQTKVKLAQ
                     LTMNQGNLLILDEPTNHIDQETKESLQDGIQKFPGTVIIVSHEQEFYQDLVQRVIEIE
                     PK"
     gene            complement(16830..>17007)
                     /locus_tag="U2K71_002233"
     CDS             complement(16830..>17007)
                     /locus_tag="U2K71_002233"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7316265.1"
                     /translation="VFMFMYFIGGAAGSLLGTTMWQQYGWLGVTVSGLVFQGCAFFFQ
                     TVVFKGKRNLENKK"
BASE COUNT         5278 a         3495 c         2910 g         5324 t
ORIGIN      
        1 tgaaaaaaca cattccacta aatttgatgc cggttttcct tgaggttttg ttccagcacc
       61 tcctttaatt tttttaagct cttgatctga acattgttta gcatttagtc tatttttttc
      121 ttccattacg attcccccta caaactataa atacttttat gtcactgatt caatatacct
      181 atttttctaa tcaaaaaatt ttgagacata tttaatatta taagtatcat taaaattgag
      241 atttatctcc ataatctgct cgatataaac catttattcc agcagttctt ccaatttcac
      301 tcaaatttct tattaattcc tcttgatatt cagtgaatct tattttatta gtataagcag
      361 taaaatatag ataattcacc aatctattta ttaaaggact gggatctaat tcatattttt
      421 gtttttctaa ttttaaatat accttttgta aaacctcaag aatatcagaa tatttaggat
      481 ctttacttat agatatatca ttatataatt catgaacaat ttgcttagca ttttttttca
      541 ttttagcact tccctggaat tgctccacct aaaaaaccac ctatagacat tcctgcaata
      601 caagtagttg ccttggccca atcgaccgta catttatttt tagtgcaata cactccattt
      661 ccataatatt ttccactatg agtggtaccc ccatagataa gttgtgtctc tttaatagac
      721 aaaattttta aatgtttcat aatctaatca cccctaaatt aatgttttta gactattatt
      781 ctaacattca ttaaatttca aaaaacaatt ccttcttgaa acgtagcaaa ataattcctg
      841 aataagaatt taatcacttt taagtatata aactatgtat attctttaat attttttgag
      901 aatgcacttc gtacataata aactttaatc acattagtct ttcataccta cgatcgatct
      961 ctttgactcg cacccataaa ttcataatag tttgtcggcg tctttgaacg tttcgaagat
     1021 gagctacgca ttctcttttt ttatagacaa aaaagataga gacgaacatg gagcttccaa
     1081 atttggatat ttgatccaat tttggaagct ccatttattt cgtgtctgtc tttttatttt
     1141 ttccagcttt cgactaattc ttggttctct ttgatccagt cttttgctgc ttgctgtgga
     1201 tcttttccat tgttgatttc caacataact ttttccatat ctttttctga ccaatggaaa
     1261 ttgtctaata ctttgtaagc ttctggctgg tcttctttca atccttttcg aaccatcgta
     1321 tggatctgct cttcgcttcc cattccgttt tcaggatctt ctaagtattt cagatcatat
     1381 ttggcaaaca tccaatgagg tgtccatcct gtaactacaa ttggttcgtg ttttttgatc
     1441 gcttgaccta gtgcaacggt cattgctcct gaagaagatg tctctacttt ccagtctttc
     1501 aagttgtcat atttttggat cgtgttttca gcagccgtaa ctacaccagc tcctggctcg
     1561 attcctatga ttttcttacc tgcctgatcg gttaactcat cgatcgaatt tacatccatg
     1621 taggctggca ctgctaaacc aacttttgct cctgtcaaat tggcacctag gtcttcgact
     1681 tgatctttat attgtgcata ttgtgaacca tgagttttag gtagccaagc tgaaaccatg
     1741 gcatctgact cgccatttga aacagatttc cacatgattg aattatctaa tggcgtcatc
     1801 gttacatcat agcccatttg tttcaatact tcaccgacta catttgttga agcaacttct
     1861 gtatcccatt caacataaga taagttgata cgttttgttt ctttggcaga tgaaaaactc
     1921 cctataccaa tcgcacctaa gatcactact actacagcac caatgatcag tccttgacgt
     1981 tttttacttt tctgtgttgt tgctcctgca gcttttttat tgtttggctg gttcaaatgc
     2041 tgtgtaaaac ggtcaatgat gattgctaag ataactaatg caaccccgtt gacaaatcca
     2101 ttcccgactt gtgctctttg caatgctgat aatacgccgc gtcctaagcc gggtgcaccg
     2161 atcatagagg caataacgac cattgaaagt gacagcattg ttgtttgatt gatccctgcc
     2221 atgatcgtac ttttagctaa aggcaattct agtttgaata gtttttgacg acttgtgctc
     2281 ccgaatgaat cagaagcttc tactaattct ttaggaactt gtcgaatccc taaattcgta
     2341 aaacgaacgg ttggtggcag agcaaagatg actgaggcaa atacccctgg aaccatcccg
     2401 ataccgaaaa atgcaactgc tggaatcaaa taaacgaatc ctggcattgt ctgcataaag
     2461 tcaaggatcg gtttgataat actttgggct ttactgcttt ttgccatcaa gatccctaat
     2521 ggtaccccaa tgatgatcga gatcacacta gataaaagaa caagtgtgac agtattcatc
     2581 aaatcattcc atagaccttg gttataaata aaccatagac cgattaatgt gaacaacgta
     2641 agtcctggac gtttcttaga aataaagaaa gctgctaccg tcaataacaa gataaacaaa
     2701 ggagctggaa tcactaacag taaatttgta atccccgaca tcactgcctg tccaatcgtt
     2761 tgtaaaaatg aaaataggcc agaaaaagtt gtcgtaaacc attctgtgat attttctacc
     2821 cagtttgcca ctggtaattg atagttcaat agattattca tgttcactca cctcgcttgt
     2881 ttctgctaat gcttcaatca cactacctcg gatgacgaca ccttttaatt ttccatcctc
     2941 tacaactgca agtggagtcg gtgagtcata aataatattg aaaatatcac ttaccaccat
     3001 gtctttccca atcaccgtca cattttcatc taggaagtcg atcaatggac gtttttgctt
     3061 cctagcttct aacgcttgat ctgcagtgat gatccctttc aggtgtctct ttctgtcaac
     3121 agccatcagc atgctgactt cttcattacg catccttgtc aatgcaacgt ttggtccatc
     3181 agattcgata ttcgtcgtca atgcaggtac catgatgttt tgagccgtaa gcactttgga
     3241 acgatcgact tcttccacaa attcacgtac ataatcattt gctggattgg tcaatatttc
     3301 ttcacctgtt ccgatctgca tgatttcgcc gtctttcatc aaggcgattc tgtccccaat
     3361 ccgtaaagct tcattcaaat catgggtaat gaaaataatc gttttttgaa cattggcttg
     3421 caggtccagt aattcgtctt gcatttctcg ccggatcagc ggatcaagag ccgagaacgc
     3481 ttcgtccatc agcaagatct caggatcatt tgctaaagct ctggctaggc caacccgttg
     3541 ttgcatccca ccagataact gactaggata ttggtcttta aatgaaagca aactagaatt
     3601 ttctaatgct ttttctgctt tttcttgtcg ttcttcttta ggtacaccac ggacttctaa
     3661 cccgtattct gtattttcca agatcgttcg atgtggaaac agaccgaagt tttggaaaac
     3721 catattgatt ttatgacgac ggacttctcg caattcttct ttgcttaact tcgatacatc
     3781 ttctccatca atatagatac tgcccgaagt tggctcaatc agacgattca atagacggat
     3841 caatgtggat ttcccactcc cggaaagccc catgatcacg aagatttctc cttcttttac
     3901 gtcaaaattc acgtcataca caccaaccgt tgcaccggtt ttctttaaaa tctccgtttt
     3961 cggctggtga tcattcatca tatctaaagc ggcttgtgtc tttttaccaa atatctttgt
     4021 taaatgtttt acttctactt ttaccaaaca aattctcctt ttttatgtaa attcattttt
     4081 ctttctgaaa aaagtgacca cactatgtta acatcatgta gtcactttgt caaaaatgaa
     4141 agaaatttgc ttgtccaaac tgaaaaaaac ttggctaaat ctttgtaaat aaaaggttta
     4201 gccaagttaa tattttttta atatttcaca caatcattct gcaagctgat gctccgggta
     4261 aaaaaagttt gtcatacgct gtagtgtata ttcatttcca ataaaatgaa cgatgtcccc
     4321 tgccgtaaat ttagcatagg ggccaggtga aacaagcagt tcttcattat gagaaattgc
     4381 gataactgtg gcgccagtcg cctgccagat attcaactcg ctaattgaac ggtccagatg
     4441 cattgctttt tctgataaag ataattcaaa cggaagtaaa ggattcatct tatgtattcg
     4501 cttcgtttga ttgaccagct gatccaataa ttgagaaaaa tgattcagct catcttgttg
     4561 cttctccaca cttgccatga tttctgcttt tgtatcttgg atggattgta catcttgata
     4621 ttgcaatagg aaatcttttg ctttttcctt cgaagcaaca aatgccccac ttccatgccg
     4681 tacttccatg attccaagat ctaccagcac attgatagcc ttgcgggtag tctcaggaga
     4741 agcattgtac gtactggcaa gtgttgagcg ggcatggatc ttctcaccca ctttatatcg
     4801 gttttcaacg atacgttcgg ctaaatcaac agcaatctgc tggtagcgcg ggcggacgat
     4861 ttttttcttt ccagacattt tctttcctct ttcttttttt actttccctc atcttactat
     4921 gctctagcaa aataagcaaa aaaggggaga atagcctctt gcaacgactg atcatcgctt
     4981 gatctgttcc ttttttactg ggcaagctgt ttgacagtca tcacaattct taccggattt
     5041 taaacgagta tagacaattg cgcctgcact tccaaagatt aacacactta ataagaatgt
     5101 cgccatccta ttcctctcct tctaatgaca atatagtata aaactggttc ttggattttg
     5161 gctgacggaa caaaacataa acgagtccta tcatcagtaa aatagcaaga tacgtcccta
     5221 acgctacctg tccaccctcc aataagtgtc ccaactgata aatcacaaaa ctaactaaat
     5281 aagctaaacc gcattgatag cctatggcga tccaagtcca tttcttggca ttcatttccc
     5341 gatggatagc cccaattgcc gcaaaacaag gcgcacaaag taaattgaag gctaacaatg
     5401 aataagctgc tgcgggagta tatgtgtgtt gtagcacttg ccagatttct ctgccatttt
     5461 cggatacttc cgcatgacca aacaaaatac caaatgtgcc aataatattt tctttagcga
     5521 ccaatcctgt aatcgctgcg actgttcctt gccagtttcc ccaaccaagc ggctgaaaaa
     5581 caactgccag gattcgcccg aaaaaggcta agatactttg gtcttcagaa acaggttgag
     5641 cataaaaatt ataagaagag ctgaaccaga tcacgatact agaaacaaag ataatcgttc
     5701 ctgctttttt aacaaaagac ttgctgcggt cataggtttg tcttaggacc gctgaccatt
     5761 gaggaaaatg atatgcgggc aattccatga taaaaggagc tgtatctcca gcaaacaaac
     5821 gtgttttttt caagctgatt ccagagaaaa taattgcagt cataccaaga aaataggcag
     5881 aaggagctac ccaagattga tgaggaaaaa aggcccctgc tactaaggca ataatcggca
     5941 actttgcaga acaaggcatg aaggttgtga ccatgatcgt caaacgacga tcttgctcgt
     6001 tttcaatcgt gcgactggcc atgacgcctg gcactccaca tcctgtagca atcagcatag
     6061 gaatgaagga ttttcctgaa agaccgaatt tcctgaaaag tcgatccatg acaaaagcga
     6121 tcctcgccat atatccgcag tcttccagaa agctcaagca taaaaacaat ataatcaatt
     6181 gcggtagaaa tcctaacact gctcccacgc ctgcgatgat gccgttcaag atcagctcct
     6241 gcatccatcc cgctacttgc cattcagcta acaatctgcc aacatgttct ggtaaccatg
     6301 taccaaacaa tgtgtcattg atccaatctg tccccattgt tcctactgtt tgaatagcca
     6361 gataatagat cagccacatc acaaatgcga agatcggcaa ggctaaccaa cgattcgttg
     6421 caatctgatc gattttgtca ctcatcgata attggaaaga atcatttgac acaacacaga
     6481 gtgtacatag ttgggtaata aattcataac gttcattgac cagaatcgtc tcactatcgt
     6541 cgtccaatag tttctctgtt aactggatca gttgctcgat ctctttttgc tgagaaactg
     6601 ctatctcgta atcctctctt accttctgat ctcgttcaaa atatttcatt gcataccagc
     6661 gtttcttgcc gctttgttct cctagtacat cttcaatttc atgtaaagct gcttctaaac
     6721 gtggatcata tgtacaggga acaggaatag aaggccgctt ttttgcagac tcctgaatct
     6781 tttcgattag tttttcgatt ccttgctttt tcaatacgct gattggtaaa actggtacac
     6841 caagaccata ggaaagcttt tctagattga ttttttttcc ttcttttttg atgacatcca
     6901 tcatattcag accaatgatc ataggaatgt ctaaatccaa taactgcgta gttaaataaa
     6961 gatttcgttc taaattggta gcatcaataa tgttgatgat cagttcaggc tgttcctgaa
     7021 ccaaataatc tcttgcaatc acttcttctg gtgtataagg agacaaagaa tagatgccgg
     7081 gtaggtcctg aatatgaatg ttcggatttt ttttcaacga tccttctttt ttttctactg
     7141 ttacgcccgg ccaattccct acatattgat tcgaaccagt caagagattg aacaagctag
     7201 ttttcccgct gtttggattt ccgactaacg cgatattcat tctttttcct ccctttgtat
     7261 caagattagt tcggcctctg actttcgtaa cgtcagttca taccctcgca ctgtcagttc
     7321 gattggatct cccagagggg ctagttttcg gataactatt tttacatttc gcgtcaatcc
     7381 catatccatt aaccgtcgct tcactgcgcc ttgtccgtgg attgctacga cgatcccagt
     7441 ctctcctgct tttaattgat caagagaagt ccagctttca gaggaagcag tctttttatt
     7501 gacaaagatt tgacctagta tttcccgact tactgccact cgatgacttt ttaccagtaa
     7561 aatggcattg ttgcctgata aattgacgac gacgatttta cttccggcaa cgatcccgat
     7621 attttgcaaa tggcgttttg ttttttcttc tgtttcgatt cgttcgactt gataaacgaa
     7681 attctgcttt gcctgcgcta aggtctgcat attgattcct ctcttgtgaa ataaatcatg
     7741 tattaaaatc attcattata tgttctaatt ttcttacgtg ttacgcaaat aaatgagaat
     7801 cattctcaca tagaatactc tcggtaattg agaatgtcaa ttatacttgt gatattatga
     7861 gcgaatcttt agtattgata gataaaaatg agaaaaaaat atttcttcat agttttccac
     7921 aattttttct ctattaaatt tgctattttc ccacacgata cttgttttca gctctttttt
     7981 caaaaacaca aaacttagta ttccacagtt tccacaacac aatatgtagt gtttgttgtt
     8041 tgactttttt actaagagtt ttatactatc tattatagat aataagaatg tgttagagga
     8101 ggcactcacc atgaacgtaa aaatcttttc taaaaataat tgtatgcaat gtaagatggt
     8161 caaacggttt ttaacagaaa atcaaattgc ttttgaagaa gtcaatatcg atgaacagcc
     8221 tgaagcagta gactggttga aagcacaagg attccaaagt gttcctgtca ttacttctga
     8281 tgcagaaaca gttatcggat tccgtcctga tcagttgcgt aaacttgcca gctaacatga
     8341 ctaaaagaat caaaacgtga tacatggatt tgaagggctt gagacagcac cagtttttgt
     8401 cgcaaacttc caaatccttt tttaaagctt aaaaacaatg caatgatttg caatcaagca
     8461 tcatcaaata cgaaatgaag gttgggcgta cgtatgagtc ttaaaaattt acaagatgtc
     8521 agttatttca agctgaataa tgaaatcaat cggccagtca atggtcaaat ccctttgaat
     8581 aaggataaag aagctttagt tgcctttttt gaggaaaatg tcaaaccaaa tacgatgact
     8641 tttccatcta ttatggaaaa aatcaattac ttgatcgatc aagaatatat agaagcagaa
     8701 ttcattcaat tgtattcacc atcatttatt gaaaagctat atcaattttt ggacgagcaa
     8761 catttttctt tcaaatcttt tatggctgct tacaaatttt attctcaata tgcgttgaaa
     8821 accaacgacg gttccgctta tctggaaact tatgaagatc gagtagcctt caatgcgctt
     8881 tactttgcga acggggatga agaattagca cttgctcttg cagacgaaat gatccatcaa
     8941 cgatatcagc cagctacacc ttctttcttg aatgctggac gtaaacgtcg gggcgaattg
     9001 gtgtcttgtt tcttgactca agtcactgac gatatgaact ctatcgggcg ctcaatcaac
     9061 tctgctttgc aattgtcccg tattggtggc ggcgtaggga ttactttatc aaatcttcgt
     9121 gaagccggcg ctcctatcaa aggatatgag ggtgctgcaa gtggtgtcgt tccagtcatg
     9181 aaattattcg aagatagttt cagctattct aatcaattag gtcagcgtca aggagctggc
     9241 gtggtttatt tgaacgtttt ccatccagac attattgctt tcctttcagc aaaaaaagaa
     9301 aatgcagatg aaaagattcg cgtaaaaact ctttctcttg gcgtgatcgt acctgataaa
     9361 ttttatgaat tgacacgtaa aaacgaagat atgtatttat ttagtccata tagtgtcgaa
     9421 aaagaatatg gtgtcccatt ctcttatgta aatattacag ctgagtatga tcatctagtt
     9481 gcaaatccaa acattcgcaa acaaaaaatc aaagcgcgtg atcttgaaaa tgaaatctca
     9541 aaattacaac aagaatctgg ctatccttac gtaatcaata ttgatacagc gaaccgcgag
     9601 aatccaatcg atggcaagat catcatgagt aatttatgtt cagagatttt acaagttcaa
     9661 acgccttctg tgattaacgg caaacaggaa tatgaagtat taggaacaga tatctcatgt
     9721 aacttaggct cgacaaatat cgtgaatttg atgcaaagcc ctaactttgg tcaatctgtg
     9781 cgtacgatga ctcgtgcatt gacatttatt acagatgcat ctgatatcga tgtcgtacca
     9841 ccgatccaaa acggaaataa gctgaatcat acgattggat taggtgccat gggcttacac
     9901 acttatcttg caaaagaaca aatggaatat ggctcagagg attcattaga ctttacaaat
     9961 atttacttca tgctgttgaa ttattggaca cttgttgaaa gcaacaatat cgctcgtgaa
    10021 agaaaacaag ttttccataa ttttgaaaaa tcagcttatg ctgacggcac ttatttcgaa
    10081 aaatacgtaa ctggagaatt ccagccaaaa tcagaaaaag tcaaagcatt gtttgaaggc
    10141 atttttatcc caactgctga agattggaat gctttgaaac aagcagtaat gaaagatgga
    10201 ctgtatcatc aaaatcgctt ggcagtagca ccaaatggct caatttcata tatcaacgat
    10261 acgagtgcaa gtattcatcc tatcactcgc atgatcgaag aacgccaaga gaagaaaatc
    10321 ggtaaaattt attacccagc gccttatcta tccaatgaga cgattccgta ttacacttct
    10381 gcttatgata tggacatgcg taaagtaatc gatgtttatg caacggcaca acaacacgtc
    10441 gatcaaggca tgagtttgac tttattcatg cgttctgaaa tcccagaagg catttatgaa
    10501 tggaagacaa caacgaagca aacgacacgt gatttgaata ttttaagaca ttacgctttc
    10561 cacaaaggaa tcaaatccat ttattacgta cgtaccttta ctgatgatgc agaagaaatc
    10621 ggaagcaatc aatgtgaaag ctgcgtgatc taatatcata gatagattcg cttattcata
    10681 ttttaattaa aggagcattc tcatgtcaga aacatattat gaagcaataa actggaatga
    10741 aattgaagat atcattgata aatccacttg ggagaagctg acagagcaat tttggctaga
    10801 tacacgtatc ccactttcaa atgacctaga tgattggcgt acattgtctc aattggaaaa
    10861 agatacagta ggacatgtat ttggcggttt gacactgcta gatactgtcc aatctgaaag
    10921 tggcatggat cagttgcgta aagatgtccg cactccccat gaagaagccg ttttgaacaa
    10981 tattcaattc atggaatccg ttcatgccaa aagttattct tctattttta gtacactgaa
    11041 tacgaaaaaa gaaatcgaag aaatttttga ctggacaaat acgaatcctt atctgcaaaa
    11101 aaaagctgag cgtatcaatg aaatctacaa acacggtaca ccgctagaaa agaaaattgc
    11161 cagcgtcttt ttagaaacat tcttgttcta ttcaggtttt tatactcctc tttattattt
    11221 aggaaacaac aaattggcta acgtagcaga gatcatcaaa ctaatcatcc gagatgaatc
    11281 tgtacatgga acctacatcg gttacaaatt ccaattagga ttcaacgagt tacctgaagc
    11341 agaacaagaa acgttaaaag actggatgta caatcttctt tatgagcttt acgaaaacga
    11401 agaacgttat acagaagaat tatacgatcc aatcggctgg acagaagaag tcaagacatt
    11461 cctacgctat aatgccaaca aagcattgat gaacctaggg atggatccac tctttccaga
    11521 tactgccaat gatgtcaacc caatcgtaat gaacgggatt tcaacaggca caagcaacca
    11581 tgacttcttc tcacaagtcg gaaacggcta tttgttaggt catgtagaag cgatgaaaga
    11641 cgacgattat ttgattggat tggaataagg atttatttta attcaaaaat ttttcgaaaa
    11701 ataaaaagag tttagcgtac agatatttta aatgtacact gaactctttt ttatgcttat
    11761 tcacttgttt cttccagcac ttttggactg tctaactttt ccgaaacatg ttccattctt
    11821 ctcaataaat agattttccg gatatttaat attatcgtct taatcactgc atagacaggt
    11881 atgcaaatca gcatgcctaa tattcctgcg acacttcctg ctccaatcaa taccaagatg
    11941 atcgtcaaag gatggatatt cattgatttc ccaaataaca acggcttgat caaatttcca
    12001 tctaattgtt ggatcactaa aatcgacaac gccatataca aagcttgaaa tggagagata
    12061 aacaaaccga caatgattgc tggtaccgct ccaataaaag ggccgacata cgggataata
    12121 ttcgtgattc cgcaaatgac cgcaagcaac aagctataag gctgatcgaa tattctcatt
    12181 gccaaaaagc tcattacacc aataatcaaa gcatctaaga tcgtactgct gatataggca
    12241 gataatgttt cgtttaattc ttttaccgtt tggcgtaact ctttacgaat cgctttcgga
    12301 aagaagtgag acaacgcatc aataaatttg tgcccatcct tgaacataaa tagcaaaata
    12361 aatggaacgg taaataacaa aataaaaaat tgcatcataa atgaaatgat ttttgagaca
    12421 cttgcagata aactgactaa tatgatattg aacaaattac taagagataa atttgctgat
    12481 gctaattgtt cttccaaatt gaaattttta aactcttccc gctgcgccaa ttcatttaac
    12541 catttgcttg tttcatctgc atacattggc aagctctggg tcaattgaat tgtctgttcc
    12601 attaactgag gcactacatt catgacagtt aaaacaacta aaatgattac gaccaaaaac
    12661 gtcaataaaa aaccgaacac tctcgggatc cgatgctttt cgagaaaaac aacgattgga
    12721 tcgaacatat aatagataaa accagcaaca agcaatggta aaaaaattgc cgaaagcatt
    12781 ttcaatattg gactaaaaat attcggcatt tgtaaaataa agtacacgcc gatgatcgtg
    12841 agtactagct ccactgtcca gaaaaataat tttgattgtt taaaacgtga ttgcatacat
    12901 ctcctccaag gtcctttgat acttctattc tatcatttaa caaagagtta cgcgctgaga
    12961 ttttcatttg cttaaaaaaa acaaaaaaaa taaacatatt ttaatgagta gacgagtact
    13021 taaacaaaaa cacggtttgg agtagtgaca aaaattatac tgcagtccct ttttccaaat
    13081 aaacggtgtt caaaagcttg catcttcgta ataagcccag ccaaacgaaa aatataagga
    13141 actattttta tttggctggg cttattactt gatgtaggac atctatttta caacctcaga
    13201 tcatttattg cgcaatactg actaaatgca actttgtgat atttttgtgt acggacaact
    13261 cctcgatcac atcggcataa gtgagccctt ttggaagatc aatcgtatag atatttttgt
    13321 acactcggta gtcatcaacg aattttacgt caaaattcac atcttcgatt acgatatttt
    13381 tctcttcaaa ataatcggta agaaaatctt tggtttcttt tctatggata aattggattt
    13441 ctaacttttt agtcgtaggt acatggatca cgcgtttcac tactgtgagt gcaatagcaa
    13501 tcccgataaa actgatgatc gcaatggaat agtaacccat gccgatggcg atccctaatc
    13561 cagcaattgc ccataaagaa gcggcagtcg taagtcccgt caccgattgt ttcgtgacga
    13621 tgatcgttcc cgcgcctaag aaaccgacac cactcaccac ttgagcaatc agccttgctt
    13681 catctgaacg aatcgttcca gcaagctgtg gatactctaa agcatctctt aatgcaccac
    13741 tagcgatctc aacttggatc aatgcaatga tacatgctcc catacaaact aaaatatgcg
    13801 tacgcatacc tgctggacga tttttatatt gtcgttcaaa accgattgct ccgccaaata
    13861 gcatagcgat ccctaaacgt aaaataatct ctggaattgt caataccata tcattcatct
    13921 aaacaccctc cagtccaata tatcatgtct gaaaggagca gacaaaaaaa gtgcaatttt
    13981 tacctcttac gaaaaaagga ctgtgacaaa atttttgtca tagtcctttt tatgaataaa
    14041 cggtgttcaa tcagctgacc ctcggtatta ctcaacaaac ggcaaaatat ggggagctat
    14101 ttttcctttg tcttctctaa tactcaggtc aaaacgctga tttcacaacc tctttagcct
    14161 actattgaaa attacaactt acttgctgct tcttcatcga tgatcacaac aacattgtcg
    14221 tgattttgca atacacttgc tggcaaatga ttcgttacag gtccttcaat catccctttg
    14281 attgcatcag ctttgctttc gccaaatgcc atcaagacga tttctttact tttcataatt
    14341 gaaccgattc ccattgaaac agcttttgtt ggtacatctt ctgctttatc gaagaatcgt
    14401 ttgtttgcat tgattgttga ttctgttaag tcaactactg aagtcgttcc gtcaaatgaa
    14461 gcaccaggtt cattgaaacc gatatgtcca ttttgcccga ttcctaagat ttggatatcg
    14521 attggatgtg catcgatgat ttgatcatac tttttgcatg ctgcttccag atcttctgct
    14581 tttccatctg gtacaaacgt ttctttgaat ggttttttat caaataattg gacatccatg
    14641 aaatgacggt agctttgttc atctgaaccg cctagtccaa cgtactcatc taagtttaca
    14701 gaagtcattt cactgaaatc caaatcgctt gaagtcattt ctttatataa agtaactggt
    14761 gtgcttcctg ttgccaatcc taatactttt gcacctttgt tcataccttc tttgatgatt
    14821 tcaaatgctt tttttccgcc ttcttcagcg ttttttacac gaatgatttc catagtgatt
    14881 gctcctttat attttggtat agcccaatta tagcattctt tttttctttt tacaataatt
    14941 agtatagacc aaattgcatt tttttcattt tcttgacaat aaataggaat ccgtgttagg
    15001 tttcataaga tggttcaatc aaacaatcca tgttgcatga aaaccaagca agtgattcac
    15061 tgtggacgct ttatgtctgc agttttttta tgaaaaagga gttattatga gtatactgac
    15121 attaaacaac attacccagc aatttggcga caaaatcctc tatgaaggtg tcgatctgca
    15181 attaaatgct ggcgaacatc ttgggctgat tggacaaaac ggagccggta aaagtacatt
    15241 gattaaaatc atcactggtg agatcttacc agatgacgga cagatccatt ggcaaaaaaa
    15301 tattcacaaa ggttacctcg atcaatatgt ggaagtcgat gaaacattaa caatcgaaga
    15361 atttctaaaa actgcgtacg caaaagaata cgaaaaggaa gaaaaaattg cttttcttta
    15421 ccaaacatat agcgagacct tcgatgattc cttgctggaa aaagctggga aactccaaac
    15481 ggaattagac caaggagtgt tttatcaaat cgacacctta gttgctgaga tgagcagcgg
    15541 gttggggatc gatgtcttag gtttagattc actgttgaaa aatctaagtg gtggacaacg
    15601 ttcaaaggtg attttagcaa aactactttt agaaaaccca gatgttctga tcttagatga
    15661 accaaccaat cacttagatg atcagcatat ccaatggctg acgcagtttc ttcaggattt
    15721 tgacggtgcg tatatcgtga tttcccatga ccaagaattt cttgatcaga tcaccaccca
    15781 tatcgcagac atcgagtttg gaaagattac taagtatact ggccatctaa aacaagcatt
    15841 gaaacaaaag gaacaaaatc gcgaaagcta tttgcgccaa tatcatgctc aacaaaaaca
    15901 aatcgaaaag accgaagctt atattcgaaa atacaaagcc ggttcccgat caacgatggc
    15961 aaaaagccga caaaaacaat tagataaaat cgaacggctg acgcctccag cttcagcaag
    16021 taaagctgtc tttgattttc cattctcgcc tattgtcacg acattagcag tggaaacaac
    16081 tgatttagtc atcggatatg atcgaccgct acttgcaccc atcaatttga ccatccgctt
    16141 tggtgaaaat attgccattc gaggcttcaa cgggattgga aaatctactt tattaaaaac
    16201 actgatcggt gagattgaaa aaataagtgg cgatttccac ttcccagata acacaaaaat
    16261 caattacttt tcacaagatc tgtactggga aaatccatta gaaacaccac tacaatattt
    16321 aagcagtcaa tttccaaaag cgactatcaa agaattacgt cgccaactag ctaaagcagg
    16381 gttagttaat caattagcaa gtgaacctct cactacatta agtggtgggg aacaaactaa
    16441 ggtgaaactt gcacagttga caatgaacca aggtaattta ctgattctcg atgaaccgac
    16501 gaaccatatc gatcaagaaa cgaaagaaag cttacaagac gggatacaga aatttccagg
    16561 aacagtaatc atcgtctctc atgaacaaga attttatcaa gatcttgtgc aacgagtcat
    16621 tgagattgaa ccaaaataaa cgtttgattg aatagttgaa aaaaaggagc tgtgacaaaa
    16681 gttttgtcac aactccttct tctgaataaa tggtggccaa ttagctgacc ttcggtatta
    16741 gctcaaaaaa cggcaaaata tgaggagcta tttttatttg gctattctta ttacttgatg
    16801 cagaacagct tttcacaacc tcttttcgtc tattttttat tttcgagatt tctctttcct
    16861 ttaaaaacaa ctgtttgaaa gaagaacgca catccttgaa aaacaagccc gctaaccgtt
    16921 acacctaacc agccatactg ctgccacatt gtcgtcccta gcaatgaacc agccgcacct
    16981 ccaataaagt acataaacat aaataca
//
LOCUS       DASHHB010000059        16371 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.061, whole genome shotgun sequence.
ACCESSION   DASHHB010000059 DASHHB010000000
VERSION     DASHHB010000059.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 16371)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16371)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16371
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.061"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..311)
                     /locus_tag="U2K71_002234"
     CDS             complement(<1..311)
                     /locus_tag="U2K71_002234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM7316266.1"
                     /translation="MPYILALDVSMGHSYSVLYKDDTCLFEDEIEHTQRGFHALLEEI
                     QHLPERPLIVFEATGIYSRPVEKFFQDHHFSYCLLNPLEAKKQMEESTLRSWKTDKSD
                     A"
     gene            complement(577..1944)
                     /gene="nhaC"
                     /locus_tag="U2K71_002235"
     CDS             complement(577..1944)
                     /gene="nhaC"
                     /locus_tag="U2K71_002235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Na+/H+ antiporter NhaC"
                     /protein_id="HEM7316267.1"
                     /translation="MNKNISLKESIAVLIVLLALLGVLIIGFQLSPHIPILAAFMCLL
                     FYGRFKKFTWDEIHDGIIKGITPGIIPILIFLLIGVLVASWILSGTIPTIMVYGFKLV
                     SVRFFLPTAFLVCAIVGVTVGSSFTTISTIGIAFLGIGHLLGFNDAMTTGAVVSGAFL
                     GNNCSPLSDTTNLAAGIGGVNLFEHIAGMRYTDFPAFVLSLIFYTILGHNSHSADLAS
                     INEMIQNMKASFWISPWTLIPLVILFGGAIKKIPAIPTLLAGSTAALVLSFIHEKSLT
                     VGKAADILMNGYVANTPDPKLDELLSRGGIMSMLGSASLILLALALGGLLIKYLIVDT
                     IVQELKEKMDRPSRLVGFTALSCIGINLIVGEQYLSIILPGETFKRSFDDVGLSKTYL
                     TRTLADSGATVNSLVPWGVSGTFIMGTMKVSALHYLPFAFFSILAPVFTILGGFLLNR
                     RRVKN"
     gene            complement(2053..3474)
                     /locus_tag="U2K71_002236"
     CDS             complement(2053..3474)
                     /locus_tag="U2K71_002236"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7316268.1"
                     /translation="MSKSEVSKMGLGTFIGLTMALCATVRSIPTLAAVGWTLIFYSIF
                     AVLFFAGPISMVSGELSTMLPQEGGPQLWVKTALGSKWGFVVAWLLWVQMFPGMVMVA
                     STLGPLLGNTFGNVELGNNHWFVLGCILVIYWIITILNLKFDMAKVGGNIGVWLGVYI
                     PVVIMFVLGVLAAFKVGLVSNGYLGDFSWSKAFPDLEHIDSLKYLAGITFIFVGIEMS
                     SVYMPRLKDATKNYTKGVFIALIGLVLLNVINAMLVANVVPDGKMELANITQPILIDC
                     QILGLPEVIGNIFSFMVFIGVLLQLSAWVTGPSKTIIQVAREGFLPPKFGFHKENKYG
                     VSRNVVLTQSIVISLFALLYGVMDDVSAVFLTLTNATTVIYCIVYILIAVSLLKMRKK
                     HPEFERPYRIGKNGNGLAWVVSCMLIFSIIVVVFATLGTATLSDALLVAAITVVMFVI
                     PLIINHFKKDSWGIEVEKSLEEK"
     gene            complement(3717..5594)
                     /gene="tdc"
                     /locus_tag="U2K71_002237"
     CDS             complement(3717..5594)
                     /gene="tdc"
                     /locus_tag="U2K71_002237"
                     /EC_number="4.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine decarboxylase"
                     /protein_id="HEM7316269.1"
                     /translation="MSESLSKDLNLNALFIGDKAENGQIYKALLNELVDEHLGWRQNY
                     MPQDMPIITPEEKSSASFEHTVNKTKDVLSEISARMRTHSVPWHNAGRYWGHMNSETL
                     MPSLLAYNFAMLWNGNNVAYESSPATSQMEEEVGMEFAKLMSYKDGWGHIVADGSLAN
                     LEGLWYARNIKSLPLAMKEVTPELVAGKSDWELMNLSTEEIMNLLDSVPEKIDEIKAH
                     SARSGKHLEKLGKWLVPQTKHYSWLKAADIIGIGLDQVIPVPVDHNYRMDINELEKIV
                     RGLAAEKTPILGVVGVVGSTEEGAIDGIDKIVALRRVLEKDGIYFYLHVDAAYGGYGR
                     AIFLDEDNNFIPFEDLKDVHYKYNVFTENKDYILEEVHSAYKAIEEAESVTIDPHKMG
                     YVPYSAGGIVIKDIRMRDVISYFATYVFEKGADIPALLGAYILEGSKAGATAASVWAA
                     HHVLPLNVTGYGKLMGASIEGAHRFYNFLNDLSFKVGDKEIEVHPLTYPDFNMVDYVF
                     KEKGNDDLVAMNKLNHDVYDYSSYVKGSIYGNEFLTSHTDFAIPDYGNSPLQFVNQLG
                     FSDEEWNRAGKVTVLRASVMTPYMNKEEHFEEYAEKIKAALQEKLEKIYADQLLASEA
                     K"
     gene            complement(5885..7141)
                     /locus_tag="U2K71_002238"
     CDS             complement(5885..7141)
                     /locus_tag="U2K71_002238"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HEM7316270.1"
                     /translation="MNIIDELTWRDAINQQTNEERLRELVEEKSISLYCGVDPTGDSM
                     HIGHLIPFMMMKRFQLAGHHPYILIGGGTGTIGDPSGRKTERVLQTMEQVQHNVDALS
                     NQMRKLFGKDANITFVNNYDWLSKISLLEFLRDYGKNFNINTMLAKDIVASRLEVGIS
                     FTEFTYQILQSIDFLHLHKTYDVQLQIGGADQWGNITAGLDLIRKLEGPEAEAFGLTI
                     PLMLKADGTKFGKTAGGAVWLDPKKTSPFEFYQFWLNQDDRDVVKYLKFFTFLSQEEI
                     EDLAKKVETEPEKREAQRRLAEEVTRFVHSEEDLKEAQKITQALFSGNIKELNAEEIA
                     QGFGKMPNVEISSTPENIVELLVSTKIEPSKRQAREDVSNGAISINGDRVTDLNFVIN
                     PSDEFDGKFVVIRKGKKNYFLAKVID"
     misc_binding    complement(7253..7458)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00230"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="T-box leader; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="tRNA"
                     /db_xref="RFAM:RF00230"
     gene            7721..9022
                     /locus_tag="U2K71_002239"
     CDS             7721..9022
                     /locus_tag="U2K71_002239"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HEM7316271.1"
                     /translation="MEKKQKIILGATSALTIVILILSLFFVIRSSTKKEPIIPEKKIT
                     EKAKEPTDDKQQLVTQAKNLAQSYDYDQAIALLEKDSKNGSTETQQLLQTLKKEKEQL
                     IDWSDPTQISHVFFHSLIVDPQKAFHSQQAQGYKDYMVTVEEFNRSIEQLYQNDFVLV
                     NLNDLIQKDEQGNLSFNGLALPEGKKPLVLSQDDVSYYEYMDNSGFPSRLVVSNNNEI
                     KNVYKEKDKEEIGDFDMVPLIDAFVKKHPDFSYRGAKGTLALTGYNGVLGYRTSKSQY
                     GDNEKTKKEIKEAKKVAEQLKKDGWSFASHTWGHLNMTQASLEDVKKDNELWQQEVAP
                     ILGKTNILIYPFGADISDWQPYSAENQKFAYLKSQGFDIFCNVDASTPAWGQLGTDFY
                     RNARINIDGIRFQSDLDGKNPILDPFINVHDVYDKEARESA"
     gene            complement(9091..9690)
                     /locus_tag="U2K71_002240"
     CDS             complement(9091..9690)
                     /locus_tag="U2K71_002240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7316272.1"
                     /translation="MNGFEKRAQEKKKEILQAAFDLMNTDIGTAGVTMDKVAENAQVS
                     KATIFKYFGSKEGLIYEVFFAFMNDLGQEAKALIAENLPFEETLIAMSENKIRYLNRV
                     NKQFYIDLMTYYTKKEKKEFSDAMDAYTQESFSIMLDLFHRGRKEGKVDLKYSDEFLI
                     LYFQAMVEGISKPEIYAKLLPYTAEWTEVLIKGIAPKKK"
     gene            9944..10825
                     /locus_tag="U2K71_002241"
     CDS             9944..10825
                     /locus_tag="U2K71_002241"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7316273.1"
                     /translation="MAEIVKVTHLKKSFGRFEALKDVSFTVHSGEVVGFIGPNGSGKS
                     TTIRTLLGIIKKDGGTATIFGQDVWEDSLSIHERLSYVPGDISLWGNLTGGAIIDLFM
                     KLHGKGSAQKKDELIQRFSLDPKKKAKNYSKGNRQKVGLIAALAVDSDLYLFDEPTSG
                     LDPLMETIFQEEVEKLKEQGKSILLSSHILSEVERLADKVVIIREGTLDEEGTLDELR
                     HLTRSTITIETEAENSHLAQLNGVHDFVQKGHLAQFAADNEAFDVILAEASKLGVKKF
                     ESIPPTLEDLFMRHYEG"
     gene            10838..12448
                     /locus_tag="U2K71_002242"
     CDS             10838..12448
                     /locus_tag="U2K71_002242"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetronasin resistance protein"
                     /protein_id="HEM7316274.1"
                     /translation="MTDKFSRWSTLFSQNLSRDWKKILIWLVGVTAFSGGFVPAFEEL
                     TSGQGLAGLYETLQNPAMIAMVGPTPASNAAEYTLGAMYANEMLLFCSLFAAVISVLH
                     VVSQTRKKEELGLIELIRSFRVGRQANSLAVIAESILINLLLAISISCTMIFFQADSI
                     TAEGAWLFGLSVGSMGILGSAVALFAAQLMPSSASSNGLSLAFLGGLYLLRAITDVAD
                     SSLSMWNPLGWSYLTYPFTTNNWIPLLYLFLFSLILIISSFVLEEHRDLNTGYIPERE
                     GKPYAKRSLLSLRGLLFYLNKGMTISWLITFGLMGAAYGSIYGNMQQFLESNDLMKQM
                     FIQSGVSIEKNFTSTIMVVLICLAAILPIASINRLFTEETHGRMNQLFATKISRNQLY
                     WTNMLLAILFAILAILISSGALGLSALASMKDAKDMDLLMFLKAGFNFLPAVLVFIGI
                     SGFLFGWLPRIGKLAYIYLAYSFALNYFGGILDLPEWFSKTAVPSWVPRMPNDSFDLS
                     VFLTLSCISLALFVLGSIGYRRRDLLEG"
     gene            complement(12525..13184)
                     /locus_tag="U2K71_002243"
     CDS             complement(12525..13184)
                     /locus_tag="U2K71_002243"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HEM7316275.1"
                     /translation="MSHDHLCVSFVPLFNHLVQQDQEKVHALVKHHYVEKGEQILAPN
                     GEPQLVIVASGSMKVYQLSASGKEQLLRVIEPGGYEGESQLLGVPNESLFGEALEKTE
                     ICILRQKDFSELLLHYPALSLKLLAINVEKLTKVQQQTTFLMMEKVEERVAVYLLDLA
                     KATGKDHFFLPMKMKELAAFIGTTPETLSRKFKLLEENGYVKRKNREVWIIDKEQLED
                     L"
     gene            complement(13467..14132)
                     /locus_tag="U2K71_002244"
     CDS             complement(13467..14132)
                     /locus_tag="U2K71_002244"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate 1-dehydrogenase family
                     protein"
                     /protein_id="HEM7316276.1"
                     /translation="MELGKSDYQLFDRPIYAFKQLKESHPTDKIEQIKKEYKEHWQKW
                     KEIQLQTAALLPDMYGMSKPKIESWTNGWNLRSHFWSAYRSESRQDENACLAVLLNQK
                     QYQIYLMYQHYKSEERYGSIAAYNQLLAILKEWSKTVDIKDYYIWPQPEHELDDHLAL
                     SDYLSDTKKQEELKKAMRDRTFQMGKLFFYPQEIEHVEQITAETLQELAPLYQKLGAE
                     KFQ"
     gene            complement(14279..14884)
                     /gene="dhaL"
                     /locus_tag="U2K71_002245"
     CDS             complement(14279..14884)
                     /gene="dhaL"
                     /locus_tag="U2K71_002245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit L"
                     /protein_id="HEM7316277.1"
                     /translation="MKLTVQEIQEWLTQFAEAINQNKQYLSDLDTPIGDGDHGNNMGR
                     GVSAYEEAFQADHPETISDTFKVFSMAMISKVGGASGPLYGSAFMNMMKATKEVDSID
                     SQEKLGEVIEQGTAGIQSRGKAEADDKTMLDVWLPVTEALKAGNLTKEIIDQAKEHTK
                     VLVAKKGRASYLGERAIGHIDPGAASSAILFSTLLDVINQP"
     gene            complement(14912..15922)
                     /gene="dhaK"
                     /locus_tag="U2K71_002246"
     CDS             complement(14912..15922)
                     /gene="dhaK"
                     /locus_tag="U2K71_002246"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HEM7316278.1"
                     /translation="MPERKDGNKLKKIMNDPSNIVEEMLEGLVKSYPELVHRVESSRV
                     VAKNQKAEQVGLVSGGGSGHEPSHAGFVGEGMLSAAVLGDVFTSPTPDQIQTAIKEAD
                     SGKGVLLIVKNYTGDALNFDMAKELAAMDDIEVESVIVDDDIAVENSTYTAGKRGVAG
                     TVLVHKIVGDAARNGASLAELKELGEKVVQATKTIGLALRAATVPEVGKPGFELGEDE
                     IEYGVGIHGEPGYRREKMQPSKVLAKELVTKILDDYSNLPKEAGVLVNGMGGTPLMEQ
                     FVFMNDVLALLEERGVNVVFRKVGNFMTSLDMQGLSLTLIDLTETQWKDSLESNVQTI
                     SW"
     gene            complement(15897..16343)
                     /gene="dhaM"
                     /locus_tag="U2K71_002247"
     CDS             complement(15897..16343)
                     /gene="dhaM"
                     /locus_tag="U2K71_002247"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS-dependent dihydroxyacetone kinase
                     phosphotransferase subunit DhaM"
                     /protein_id="HEM7316279.1"
                     /translation="MKAKPIVSNYVKERKILVKKSILLISHSQKVTDGIKEMIEQMQK
                     SEEVQIFSLGGINGEIGSDPMKIVEAVNESSDAASYFVFADIGSAVLNSELAKDMLEE
                     EQQQRYHLVDAPLVEGAFAAAITAGITDDTQQILTEAQNAGKKGWE"
BASE COUNT         4682 a         3417 c         2976 g         5296 t
ORIGIN      
        1 tgggcatccg atttatctgt tttccaactg cgtaaggtgc tttcttccat ctgttttttc
       61 gcctctaacg ggtttaacaa acagtaagaa aagtgatgat cttgaaagaa cttttcgact
      121 ggtctggaat aaatccccgt tgcttcaaaa acaatcaatg gacgctcagg caagtgctgg
      181 atctcttcga gtaatgcatg aaatcctctt tgagtgtgtt caatctcgtc ttcaaataag
      241 caggtgtcgt ctttgtatag gacactgtag ctgtgtccca ttgaaacatc taatgcaaga
      301 atgtaaggca tttgttttcc tcctttttaa gttttgaaag aactttactg cttcatttcg
      361 attccaattt cctatacacg ggctcatggc cccaacatac gtaaacctga ttctaaaatg
      421 acagtaaaga agttcggttt ttgataggga ctcgtagtcc tcaaggcttt gttcgaactt
      481 tctttcactc ttcactataa tctaaaaata gaaaatagag gagaacatca ttcgtcaatg
      541 atgttcccct ctaatcttag tatgtttttc cttccttcaa ttttttacac gtcgacgatt
      601 caataaaaaa ccgcctaata ttgtaaagac aggtgcaaga atcgagaaaa aggcgaatgg
      661 caagtaatgc aacgcgctaa ctttcatcgt tcccataatg aatgtgccgc tgactcccca
      721 cggtacgaga gaattgacgg tcgcacctga atccgctagt gttcgtgtca aataggtttt
      781 tgataagcca acgtcatcaa aagaacgctt gaatgtttct cctggaagaa tgatggacag
      841 atactgttct ccaacgatca gattgattcc gatacagctc aaggcagtaa acccgactaa
      901 acgagaaggt cggtccatct tttcttttaa ttcttgaacg atggtatcaa cgatcagata
      961 tttgatcagt aacccaccta atgccaatgc aagtaaaatc aaactagcag aacctaacat
     1021 actcatgatc ccgcctcgag aaagcagttc atctagcttt ggatcaggtg tatttgcgac
     1081 ataaccattc atcaagatgt ctgctgcttt tccgaccgtc aaacttttct catggataaa
     1141 agaaagaacg agagcagctg tcgatcccgc tagtaatgta gggatagccg gaattttttt
     1201 gattgccccc ccaaataaaa taactagtgg aatcaatgtc caaggagaga tccaaaagct
     1261 cgctttcatg ttttggatca tttcattgat actagctaga tcggctgaat gactgttatg
     1321 tcctaatatc gtataaaaaa tcaacgaaag gacgaatgct gggaaatccg tataacgcat
     1381 accagcgata tgttcgaata gattaactcc tccaataccc gctgccaagt tggtcgtatc
     1441 tgataaaggc gagcagttat tccctaaaaa ggcaccggaa acgactgcgc ctgtagtcat
     1501 tgcatcattg aatccaagta aatgaccgat ccccaaaaaa gcaattccaa tcgtcgaaat
     1561 cgttgtaaat gaactgccga cagtcactcc gacaattgca caaacaagaa atgctgtcgg
     1621 taaaaagaaa cgcacagaaa caagtttaaa tccataaacc ataatcgtag ggatggttcc
     1681 agataaaatc catgaagcaa ctaagacacc aatcaaaaga aaaatcagga ttggaatgat
     1741 ccctggagtg attcctttga tgatcccgtc atggatctca tcccatgtga actttttaaa
     1801 tctgccataa aacaacaaac acatgaaagc tgcaaggatc gggatatgtg gcgagagctg
     1861 gaatccgatg atcagtactc ctaggagtgc caacaacacg atcaatacag cgatactttc
     1921 ttttaaagaa atatttttat tcatcgtcga tctcctattt caaatgaaaa ggccctgcca
     1981 tgaaaaaact attcacttgg caggacctcc tactaaatca gcgacaaaac gtcggctgaa
     2041 agacttattt ttttattttt cttctaaact tttttctacc tcaatccccc aagaatcttt
     2101 cttgaagtga ttgatgatca atggaataac gaacatgacc actgtgatag ctgctactaa
     2161 taatgcatca ctcaatgtcg ctgtacctaa tgtagcaaat acgacaacga tgatagagaa
     2221 gatcagcatg cagctgacta cccaagctaa tccgtttccg tttttaccaa ttcggtaagg
     2281 acgttcgaat tctggatgtt tcttacgcat tttaagcaat gaaacagcaa tcaaaatgta
     2341 cacgatacaa taaataacag ttgtagcatt tgtaagtgtc aagaatacag cacttacgtc
     2401 atccattaca ccataaagca aagcaaataa tgaaataacg attgattgtg ttagaacgac
     2461 atttctagat acgccgtatt tgttttcttt atggaatccg aattttggtg gaaggaatcc
     2521 ttcacgagct acttggatga ttgtttttga tggtccagta acccaagctg ataattgtaa
     2581 aagcacacca ataaatacca tgaagctgaa gatatttccg atcacttctg gtaagcctag
     2641 gatttgacag tcaatcaaga taggctgcgt gatatttgct aattccattt ttccatctgg
     2701 tacgacattt gctacaagca tcgcattgat gacattcaat aaaactaatc cgatcaaagc
     2761 gataaatacg ccttttgtat aattttttgt tgcgtctttc aaacgaggca tgtaaacaga
     2821 actcatttcg atcccaacaa aaataaatgt gataccagct aagtatttca atgaatcaat
     2881 atgttccaag tcagggaaag cttttgacca tgaaaagtca cctagatagc cgtttgagac
     2941 tagtccgact ttgaaggctg ccaaaacacc taaaacgaac atgatgacta ctgggatata
     3001 tacccctagc catacaccga tgttcccgcc gacttttgcc atatcgaatt tcaagttcaa
     3061 gatcgtgatg atccagtaga taactaaaat acaacctaaa acgaaccagt gattattgcc
     3121 aagttctaca ttaccaaatg tgtttcctaa caatggtcct aaagtagaag cgaccattac
     3181 catacctggg aacatttgca cccataatag ccatgcaacg acaaatcccc atttgctgcc
     3241 cagtgctgtt tttacccata actgaggacc gccttcttga ggaagcatcg tggaaagctc
     3301 accggaaacc attgatatcg gaccggcaaa aaacaaaact gcaaaaattg aataaaaaat
     3361 caatgtccaa ccgactgcgg ctaacgtagg aatactccga accgttgcac acagtgccat
     3421 tgtcagaccg ataaaggtcc caagacccat tttacttact tcagatttac tcaccgttga
     3481 cactctcctt gattttttgg ataaaataac ggtgtctttg aagtagtttc agcaataaac
     3541 gaaacaattc agcgaatatt tgaggggatt ctttgagttt attgcttgaa gggtttcttc
     3601 aaagacaacc tcaaaacaaa gggatcgaag agcttaggtc tccccttgct cttcagatcc
     3661 aaacatgcgt cagaaacagt tagtattcaa gctgttttct aacatgatta ccttgcttat
     3721 tttgcttcgc ttgccaataa ttggtctgcg tagatttttt ccaatttttc ttgaagagct
     3781 gctttgattt tttcagcata ttcttcaaag tgttcttctt tgttcatgta tggtgtcatt
     3841 acgctggcac gcaatacagt tacttttccg gcacggttcc attcttcatc tgagaatcct
     3901 agttggttca caaattgtaa tgggctgttg ccatagtctg ggatagcaaa gtcagtatgt
     3961 gaagttaaga actcattacc gtaaatgctt cctttcacgt atgaagaata atcatatacg
     4021 tcatggttca atttattcat tgcaactaag tcatcgttgc ctttttcttt gaatacatag
     4081 tctaccatat tgaaatctgg ataagttaat ggatgaactt cgatttcttt gtctcctact
     4141 ttaaatgaca agtcattcaa gaagttgtag aaacgatgtg caccttcgat tgatgcaccc
     4201 attaatttac catatcctgt gacattcaaa ggtaagacat ggtgtgctgc ccaaacgctg
     4261 gctgctgttg cccctgcttt tgaaccttcc aagatataag ccccaagtaa tgctgggata
     4321 tctgcgcctt tttcaaatac atatgtcgca aagtaagaga taacgtctct catgcggata
     4381 tctttgatga cgataccacc ggcagaatat ggaacatagc ccattttatg tgggtcaatc
     4441 gttacagatt ctgcttcttc gattgcttta tacgcactgt gtacttcttc taagatataa
     4501 tctttgtttt ctgtaaagac attgtatttg taatgaacat cttttaaatc ttcaaatgga
     4561 atgaagttgt tgtcttcgtc taagaaaatc gcacggccgt atccgccata agcagcatct
     4621 acgtgtaagt agaagtaaat accgtctttt tctaatacgc gacgcaatgc aacgatttta
     4681 tcgataccgt cgattgctcc ttcttctgtt gatcctacaa caccgactac acctaggatt
     4741 ggtgtttttt cagctgctag accacgaacg attttttcaa gttcgttgat gtccatgcga
     4801 tagttgtggt caactggcac tggaatcact tggtctaaac cgataccgat gatgtcggca
     4861 gcttttagcc atgaatagtg tttagtttgt ggaaccaacc attttcctaa tttttctaaa
     4921 tgttttccgc tacgtgctga gtgagctttg atttcgtcga ttttttctgg gacgctgtct
     4981 aacagattca tgatttcttc tgttgacaag ttcattaatt cccaatcgct ttttccagca
     5041 actagttcag gtgtcacttc tttcattgca agcggcaatg atttgatgtt gcgggcatac
     5101 caaagtcctt ctaagtttgc taaagaacca tcagcaacga tgtggcccca gccatcttta
     5161 tagctcatta attttgcaaa ttccattcct acttcttctt ccatttggct tgttgcagga
     5221 gaagattcat aggcaacgtt attaccattc catagcatag caaaattgta cgctagtaaa
     5281 gatggcatca atgtttcaga gttcatatga ccccagtaac gtccggcatt atgccaagga
     5341 acggaatggg tacgcatccg cgctgaaatt tctgatagaa catcctttgt tttattcaca
     5401 gtgtgttcaa aactagcact gcttttttct tctggcgtaa taataggcat gtcttgaggc
     5461 atgtagtttt gacgccagcc taaatgttca tccaccaatt cattcaacaa agctttatag
     5521 atttggccgt tctcagcttt gtccccaatg aatagggcat ttaggtttag atctttcgac
     5581 aatgattcac tcatagtgaa accctcctaa aaaaatattt aaattttgtc gagccctaca
     5641 ttacacctgt tcttctattc tgtaaactaa gttttggcct gatttttaat catattttaa
     5701 tatgtatcaa ttatccgaat tgttcatact tttgaaataa ttaaaaaaaa cgagggaatt
     5761 tacaaatgta aactccctcg caaagattgt attaacgaca accagcgttc tattttagga
     5821 tgggtttcaa aattggaatg ttgtaatagt atttcccttt ttcaaggtaa aaacttacta
     5881 aattctaatc aattactttg gccaaaaagt aatttttctt accttttcga ataaccacaa
     5941 acttaccatc gaattcatcg gatggattta tgacaaaatt taaatcggta acgcggtcac
     6001 cgttaatact tatagctccg tttgaaacat cttcacgtgc ttgacgttta gatggttcaa
     6061 tttttgtgga aacaagcagt tccacgatat tttcaggtgt gcttgaaatt tcaacgttcg
     6121 gcattttacc gaatccttgc gcgatttctt ccgcatttag ttctttaata tttccagaga
     6181 acaatgcttg cgttattttt tgcgcttctt tcaagtcttc ttcactatgc acaaatcttg
     6241 taacttcttc tgccaaacgg cgttgtgctt cacgtttctc aggttctgtc tctacttttt
     6301 tcgctaagtc ttcgatttct tcttgagaca agaaagtaaa gaatttcaag tatttcacta
     6361 catcgcgatc atcttgattc aaccagaatt ggtagaactc aaatggtgaa gttttcttcg
     6421 gatcaagcca aacggcaccg cctgcagttt ttccgaattt cgttccgtca gccttcagca
     6481 tcaatgggat cgttaaaccg aacgcttctg cttctggtcc ttctaatttt cggattaggt
     6541 ctaatccagc tgtgatattt ccccattggt ctgctccacc gatttgcaat tgcacatcgt
     6601 atgttttatg cagatgcaaa aagtcgatag attgcaggat ttggtaagta aactctgtaa
     6661 atgagattcc tacttccaaa cggctggcaa cgatatcttt tgctaacatc gtgttgatgt
     6721 tgaagttttt accgtagtct ctcaaaaatt caagtaaaga gatttttgac aaccagtcgt
     6781 agttgttcac aaatgtgata tttgcatctt taccaaacaa ttttctcatt tgattggaaa
     6841 gtgcgtccac gttatgttgc acttgttcca tcgtttgcaa cacacgttct gttttgcggc
     6901 cgcttgggtc gccgatcgtt cctgttccgc cgccaatcag aatatatgga tgatggcctg
     6961 ccaattggaa tcttttcatc atcataaatg gaatcaaatg tccgatatgc atcgaatcac
     7021 cagttggatc aacgccgcaa taaagtgaaa tgctcttttc ttcaacgagc tcgcgaagtc
     7081 tttcttcgtt tgtttgctga ttaatggcgt cgcgccaagt taattcatca ataatattca
     7141 ttttactgac ctccgtgtag tttttttatg aaagcaggaa cctgctagca tgtgtaatta
     7201 attatttctt caggatagga taggaaataa aaaaagtccc ttggcatata agccaaggga
     7261 cgaaatctcg cggtaccacc caatttgtat gtctagatat ccaacatacc tctcgaacat
     7321 ttaacgctgt cgcgtggcaa tcactgccaa agactcctga aatgtaattc gctttaaagc
     7381 atgtactggt ttgcagcgac caccagcttt ctgaaacatt ggctttaaca actacttcgt
     7441 ttcgatcttc gtcttgttat tattaatatg tgtctatcat agagcgcaac tttttctttg
     7501 tcaacagaaa aaggatattt tttttaaatt cctcattttt tcttttttga atccgctcta
     7561 attccgaaca ttttattttc aattattgtt aaaagaaata actttttctg tattttttct
     7621 gcaatttatc gagaagtcac tttactttgc ctcttattct ttcttcgtta caataaggaa
     7681 tgggacaagt aaaaaaatga aaaagcaagg gacgaggtat atggaaaaaa agcaaaaaat
     7741 tattttagga gccacttctg ctttaactat cgttatttta attctaagtt tgttttttgt
     7801 catccgatca tctacaaaaa aagaaccaat cattcctgaa aaaaaaataa cagaaaaagc
     7861 aaaagaaccg acagatgata agcaacaact agttactcaa gcaaaaaatc tggctcaaag
     7921 ctacgattat gaccaagcga ttgcgttatt ggaaaaagat agtaagaacg gatctactga
     7981 aacacaacag cttcttcaaa cactaaaaaa ggagaaagaa caactgatcg actggtctga
     8041 tcctactcag atctctcatg tctttttcca cagcttgatc gtggacccgc aaaaagcctt
     8101 ccactctcaa caggcacaag gatacaaaga ttatatggtg actgttgaag aattcaaccg
     8161 ttcgatcgaa caactttatc aaaatgattt tgttttagtt aatttgaatg atttgatcca
     8221 aaaagacgaa caaggaaatc tttcctttaa cgggcttgcg ctacctgaag gaaaaaaacc
     8281 attggttctt tcacaagatg atgtcagcta ttatgaatat atggataatt ccggttttcc
     8341 tagccgattg gtcgtatcaa ataacaatga aatcaaaaat gtctataaag agaaagataa
     8401 agaagaaatc ggtgactttg acatggttcc tctgatcgat gcctttgtca agaaacatcc
     8461 tgatttttct tatcgaggcg caaaaggtac cttggccctg acaggttaca acggagtttt
     8521 aggttatcga acatcaaaat ctcaatatgg agacaatgaa aaaacaaaaa aagagattaa
     8581 agaagctaaa aaagtggcgg aacagttgaa gaaagacggc tggagctttg cttcccatac
     8641 atggggtcac ttgaatatga ctcaagcttc actagaagat gtaaaaaaag acaatgaatt
     8701 gtggcagcaa gaagttgcac ctatcctagg aaaaacaaat attctgattt atccatttgg
     8761 tgcagacatc agcgactggc agccttattc agcagaaaat caaaaatttg cttacttgaa
     8821 gagtcaaggt ttcgatattt tctgtaatgt cgatgcttct acaccagctt ggggacaatt
     8881 aggtacggat ttctatcgaa atgctcggat caatatcgat ggcattcgtt ttcaatctga
     8941 tttagacgga aaaaatccta ttcttgatcc attcatcaat gtccatgatg tctatgacaa
     9001 agaagcacgc gaatctgctt gattttacgt catttatgaa ccaaaaaagc cgaactgaca
     9061 ttttatcgtc agtttcggct tttttatgct ttatttcttc ttcggtgcga ttcccttgat
     9121 caggacttct gtccattctg ctgtatatgg aagcagtttt gcgtatatct ccggcttaga
     9181 aattccttct accattgctt gaaaatagag gatcaagaac tcatccgaat atttcaaatc
     9241 cacttttcct tctttccgcc ctcgatggaa aagatcaagc atgatactga aactttcttg
     9301 cgtatacgca tccatcgcgt cagaaaattc ttttttttct ttttttgtat agtatgtcat
     9361 caaatcgata taaaattgtt tgttcactcg attgagataa cggattttgt tttcactcat
     9421 ggcaatcagt gtttcttcaa atggaagatt ttccgcaatc aacgctttcg cttcttgccc
     9481 taaatcattc ataaatgcaa aaaatacttc ataaatcagc ccttccttgc tgccaaaata
     9541 tttaaaaatc gtcgctttac ttacctgagc gttctcagct accttatcca ttgtcactcc
     9601 agcggttcct atgtctgtgt tcatcaagtc aaaagctgct tgtaaaatct ctttcttttt
     9661 ctcttgtgct ctcttttcaa aaccgttcat ttgtagagcc tcccgtaatt ttaaactttt
     9721 ataatatata tagttcaaat ttattctgag gtttattata actatctatt gagaaagtat
     9781 caagctagca attccttgtt tatgaattca ttgacagaaa tttataaaaa gaatattatg
     9841 tactaaaata ataaaaaaag ttttataaaa aggtataaaa cctttttatt aaactattta
     9901 atcttgttta gtatatattt ttagaaaaag gagtggggtt tttatggcag aaatcgtcaa
     9961 agtcacacat ttgaaaaaga gctttggtcg tttcgaagct ttaaaagatg tctctttcac
    10021 cgtacattca ggtgaagtcg ttggatttat cggaccaaat ggttctggaa aatccacgac
    10081 gattcggaca cttttaggga ttataaaaaa agacggcgga acggcaacga tttttgggca
    10141 agatgtatgg gaagatagtt tatctatcca tgaacgtctt tcttatgttc caggcgatat
    10201 ctctttgtgg ggaaatttga ctggaggagc gatcatcgat ctcttcatga agcttcatgg
    10261 aaaagggagt gctcaaaaaa aagatgaact gattcaacgt ttctctcttg atcctaagaa
    10321 aaaagcaaaa aactattcaa aaggaaatcg ccaaaaagtg ggcttgattg ctgctttggc
    10381 tgttgattcg gatctctatt tatttgatga gcctacttct ggtttagatc ctttgatgga
    10441 aacgattttc caagaagaag ttgaaaaatt gaaagaacag ggcaaatcca tcctactttc
    10501 ttctcatatt ctcagtgaag tcgagcgact agcggataaa gtcgtgatca tacgcgaagg
    10561 aacactcgat gaagaaggaa cactcgatga attgcgtcat ttgactcgtt cgaccatcac
    10621 tatagaaaca gaagcagaga atagtcactt agcacaattg aacggtgtcc atgactttgt
    10681 gcaaaaaggt cacctcgctc aatttgctgc tgataacgaa gctttcgatg tgattttagc
    10741 tgaagcgtcc aaattaggtg tgaaaaaatt tgaatctatt ccaccgactc ttgaggattt
    10801 attcatgaga cattatgaag gatgaggagg aagcgaaatg actgataaat tttcacgctg
    10861 gtccaccctt ttttctcaaa accttagccg tgactggaaa aagatcctga tatggctagt
    10921 cggtgtcact gctttttctg gtggttttgt ccctgcgttt gaagaattga cttccggaca
    10981 aggattagct ggattatacg aaaccttgca aaacccggca atgattgcaa tggtcggacc
    11041 aactccagct tcgaatgctg ctgagtatac tttaggagcg atgtatgcaa atgaaatgtt
    11101 gcttttttgc agtttgtttg ctgctgtgat ctctgtttta cacgtggtga gccaaacgag
    11161 aaaaaaagaa gaacttggtc tgattgaact tattcgttcc tttcgagtcg gacgtcaagc
    11221 aaactcttta gctgtcatcg ctgaaagtat tctgatcaat ttgcttttag caatatcgat
    11281 cagctgtacg atgatttttt tccaagcgga ttcgatcact gcagaaggtg cttggctttt
    11341 tgggctatct gtaggtagta tggggatttt ggggagtgcc gttgcactgt ttgctgcaca
    11401 actgatgcca agttcagcca gttcgaatgg tctgtcttta gcgtttttag gtggacttta
    11461 ccttctacga gcaataacag atgtagctga ttcctccctt tcgatgtgga atcctttagg
    11521 ctggtcttat ttgacctatc catttacaac taacaactgg attcctttgc tttatttgtt
    11581 tttgttcagt ctaatcttga taatcagttc atttgtttta gaggaacatc gagatttgaa
    11641 caccggctat atccccgaac gtgaaggaaa accgtatgct aaacgttctc ttttatcctt
    11701 gcgtggttta ttattctatc taaataaagg gatgacaatc agttggctta tcacttttgg
    11761 tttgatgggg gcggcatacg gctctatcta cgggaatatg caacagtttt tagaaagtaa
    11821 cgacttaatg aaacagatgt ttatccaatc tggcgtctca attgaaaaaa actttacaag
    11881 cacgatcatg gtcgtcttga tttgtctagc tgctatttta ccaattgcta gcatcaatcg
    11941 tttatttaca gaagaaaccc acggacgtat gaatcagctg ttcgcaacaa aaatttctag
    12001 gaaccaactt tattggacca atatgttact agctattcta tttgctatat tggcaatcct
    12061 gattagttct ggtgcactag ggctttctgc tttggcttcg atgaaggatg cgaaagacat
    12121 ggatctattg atgtttctta aagccgggtt caatttcctg cctgccgttt tagtgtttat
    12181 cggaattagt ggctttctat tcggatggct tcctcgaatc ggtaaacttg cttatatcta
    12241 cttagcttac tcctttgcac tcaattactt tggcggaatt ttggatttac cagaatggtt
    12301 ttccaaaaca gctgtcccta gctgggttcc acggatgcca aacgattctt ttgatctttc
    12361 cgtgttcctt acgttatcat gtatcagcct tgcactgttc gtccttggtt ctattggtta
    12421 taggcgaaga gacttactag aaggataata aaaaagcatg tacaatttca tgctagcctc
    12481 caaatctggg tcaaaaatcc aacatttgga ggtcattttt ttatttacaa gtcttctaac
    12541 tgctctttat caatgatcca gacttctcga ttctttcttt ttacataacc attctcctct
    12601 aataatttaa attttctaga taaagtttcg ggggtcgtac cgataaatgc agcaagttct
    12661 ttcattttca ttggcaggaa aaagtgatcc ttccctgtcg ctttagctaa gtcaagcaga
    12721 tagacagcga ctcgttcttc tactttttcc atcatcaaaa aagttgtctg ctgctgtact
    12781 tttgttaatt tctcaacgtt gattgctaaa agtttcagac taagggcggg ataatgcagt
    12841 aagagttccg agaaatcttt ttgtctcaat atgcaaattt ccgttttttc tagtgcttcg
    12901 ccaaataaag actcatttgg aacgcctagc aactggctct ctccctcata tccgcccggc
    12961 tctattactc gtaaaagctg ttcctttccg ctagcagata attgatacac tttcatactg
    13021 ccggaggcca cgatcactaa ttgaggttct ccattcggcg caagaatctg ttctcctttt
    13081 tccacatagt gatgtttgac aagtgcgtgt actttttctt gatcttgctg tactaagtga
    13141 ttgaacaaag gaacaaatga aacacataaa tgatcatgac tcataatctc tcctccttct
    13201 aaacagctat ttctaataaa gaaacataac ttcccttatt ccaactgata attttctttt
    13261 atcatagcat aaacgtcaag cgaaccagat accagcaaat agagtggatt tctttttgtc
    13321 ccttgcttta ttttccttct tttgagacct taagtaattg attcctatca attttttaaa
    13381 tatttacttt ttgaataatc aaaaacaaac cttcactcgc aagccgagag taaggtttgt
    13441 ttttcgcatt gcttatttcg tgttgtctat tgaaattttt ccgcacctag cttttgatat
    13501 agtggtgcta gttcttgtaa ggtttcagcg gttatttgct caacgtgttc gatttcttgc
    13561 ggatagaaga atagtttacc catttgaaag gttcggtctc tcatcgcttt tttcaactct
    13621 tcttgtttct ttgtatcgga caaatagtca gacaaagcta agtggtcgtc aagttcatgt
    13681 tctggttgcg gccatatgta ataatccttg atatcgactg ttttgctcca ctctttcagt
    13741 atcgcaagca actggttata cgctgcaatg ctaccatatc gttcttcgct tttataatgt
    13801 tggtacatca aatagatttg gtattgtttt tgattcaaca aaactgctag acaggcattc
    13861 tcgtcttgac gagattcgct tcggtacgcg ctccaaaaat gactgcgtaa attccagcca
    13921 ttcgtccagc tttcgatttt aggtttgctc atgccgtaca tatcaggcaa aagtgcagct
    13981 gtctggagct gaatctcttt ccatttttgc cagtgttctt tatattcttt cttgatttgt
    14041 tcaatcttat ctgttggatg gctctctttg agctgtttga atgcgtagat tggtcgatca
    14101 aacaactgat aatctgattt tccaagttcc attgtttcgc ctctttccgt tatcttttgt
    14161 attctacctg aattttgcga tatggacaaa tagaggttgt gaaaaagctg ttctgcatca
    14221 agtcataaga acagccaaac aaaaaactgc gatgagcatc accgcagttt tattgaactt
    14281 aaggttgatt gatcacatct aaaagcgttg aaaataaaat ggcactagaa gcagctcctg
    14341 ggtcgatatg tccgattgca cgctctccta gataggaagc tcgtcctttt ttagctacca
    14401 agactttggt gtgttctttt gcctggtcaa tgatttcttt tgttaaattc cctgccttca
    14461 atgcctctgt gactggcagc caaacatcta acatcgtttt atcgtcagct tctgcctttc
    14521 ctcggctttg aatacccgct gttccctgct cgatcacttc gcctaatttt tcttgagaat
    14581 caatagaatc gacctctttt gtcgctttca tcatgttcat gaatgcagat ccgtataacg
    14641 gaccagaagc cccaccgact ttcgatatca tcgccataga gaatactttg aatgtatcgc
    14701 tgattgtttc aggatgatcg gcttgaaaag cttcttcata agctgaaacc cctcttccca
    14761 tgttatttcc atggtctcca tcgccaatcg gggtatctag atcactgaga tattgtttgt
    14821 tctgattgat tgcctctgca aattgcgtga gccattcttg gatttcttga acggttagtt
    14881 tcatattatc acccttttat cttgagattt tttaccatga aatcgtctgt acattgcttt
    14941 ctaaagaatc tttccattgt gtctccgtca agtcgatcag tgttagagaa agaccttgca
    15001 tatctagtga ggtcataaaa ttaccgactt ttcggaaaac tacattgacg cctctttctt
    15061 ccaacagagc taaaacatca ttcatgaaca cgaactgttc catcaaaggc gtcccgccca
    15121 ttccgttgac tagtacacct gcttcttttg gtaaattcga gtaatcatct aaaatcttag
    15181 taacaagttc ctttgccagt actttagaag gctgcatctt ttctcgacga tagcctggct
    15241 caccgtggat accaacacca tactcgatct catcttctcc tagttcgaat cctggtttcc
    15301 ctacttctgg tacagttgct gctcgaagcg ctagaccgat cgtcttagtt gcctgtacga
    15361 ccttttctcc cagttctttc aactcagcta gggaagcacc gtttcttgct gcatcgccca
    15421 cgattttgtg tacaaggaca gtacctgcaa ctccgcgttt tccagcagta tacgtactat
    15481 tttcaacggc gatatcatca tctacgatca cactttctac ttcaatatcg tccatagcag
    15541 ctaattcctt tgccatatcg aagttcaatg catcccctgt ataattctta acgatcagca
    15601 aaactccttt tccactatcg gcttccttaa tagccgtctg aatctgatct ggagttggag
    15661 aagtaaacac atcaccaagc accgctgcac ttaacatccc ttcccctaca aacccggcat
    15721 gggatggttc atgtccgctt cctccaccag aaaccaaacc aacttgttcc gctttctgat
    15781 tcttggctac aacacgggaa gattctacac gatgaacaag ttctggatag ctttttacta
    15841 atccttccag catttcttcg acaatattgc ttggatcatt catgattttt ttcaatttat
    15901 tcccatcctt tctttccggc attttgtgct tctgttaaga tttgctgagt atcatctgtt
    15961 ataccggcag tgatggctgc tgcaaaagct ccttccacta aaggcgcatc caccagatga
    16021 taacgttgct gctgctcttc ttccaacatg tcttttgcca gttccgagtt caataccgcg
    16081 ctaccgatat ccgcaaaaac aaaataacta gctgcatcgc ttgattcatt gactgcttct
    16141 acgattttca tcgggtctga tccgatctcg ccatttattc cgccgagaga aaaaatttgc
    16201 acctcttcgg atttttgcat ctgctcaatc atttctttaa taccatctgt tactttttga
    16261 ctatgagaaa taagcaaaat actttttttc acaagaatct tcctttcttt tacatagttg
    16321 ctaacgattg gtttcgcttt cattaatgaa caattcaagt atataataaa t
//
LOCUS       DASHHB010000060        15230 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.062, whole genome shotgun sequence.
ACCESSION   DASHHB010000060 DASHHB010000000
VERSION     DASHHB010000060.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 15230)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15230)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15230
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.062"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            278..787
                     /locus_tag="U2K71_002248"
     CDS             278..787
                     /locus_tag="U2K71_002248"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM7316280.1"
                     /translation="MVQGIERGVYVVDKIQFNQVIEEFIKVNNLMTHIQKRPIRITND
                     LKLSTSMIHLIDVIGNYPETSITELADRLGVTKGAVSQQIPTLEKMNLISISQKKGNK
                     KTKLVSLTKYGRKVYESHNYLHEELYSSINEALESFSPDQISTIKQILEKVSYTINEY
                     QVSLTRKED"
     gene            880..2042
                     /locus_tag="U2K71_002249"
     CDS             join(880..1156,1156..2042)
                     /locus_tag="U2K71_002249"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEM7316281.1"
                     /translation="MSRRQRRTYSKEFKQQVVDLYLAGKPRAEIIRDYELTTSAFDKW
                     VKQAQTTGSFQEKDNLIPEQVELIALRKKNKQLEMENDILKQAALILGPKRQVIDTNK
                     HKYSISAMCKVLNISCQTYYYQAKPVEDESELEELVQEEFIRNRKAYGTRKLKQCLAN
                     LGLQVSQRRIGRMMKQRGLRSTYTLAHFKFHSSTCNEAKTANILDGTFLQEKSLEAIV
                     TDLTYVRVGKKWQYICLILDLFNREIIGYSCGEKKDATLVKEAFAKIPYSLNNVQLFH
                     TDRGKEFDNQTIDEILWAFNITRSLSKKGCPYDNAVAESTYKSVKVEFVHQYRFETLA
                     NLRLELFDYVHWWNTIRLHGTLGYETPISFRQQRLAQRILDNELGCDTGGEAP"
     gene            2322..3302
                     /locus_tag="U2K71_002250"
     CDS             2322..3302
                     /locus_tag="U2K71_002250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7316282.1"
                     /translation="MKKIGIREVAKEANVSPTTVSRVLNDRGYLSEETKRKVFDAMKK
                     LEYYPNELARALFKNETYTVGLIFPTITNPFHAELIQDIEFELSSRGYKVLLCNSLNN
                     PTKEKEYLTMLRKNQVDGIIVGTHNKDIADYDIPGLPIVAIDRSLGENTVTVSCDNYR
                     GGELATQLLVNHGCQNILCIRGDSKIKLPANSRTLAYKDVLNKFMLEPLVLEVPFVKE
                     INEKNEIIKTYLETHPNIDGVFAGDDLLASLAINNLGSSGKRVPKEVKVIGFDGAQQT
                     LIYNPELSTIQQPIDQISKVAVKKLLNMIKGEKETDELSLPVKLIKKNTA"
     gene            3424..4842
                     /locus_tag="U2K71_002251"
     CDS             3424..4842
                     /locus_tag="U2K71_002251"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="family 43 glycosylhydrolase"
                     /protein_id="HEM7316283.1"
                     /translation="MEKLLIKSNNHQLYYQPKDVWVGDVMPYGKEGKFFIYHQRDTRN
                     PVPFGEPFGWALATTKDFVHFEDFGESLLRGDDNERDQFIYAGSVFEANGKVHAFYTG
                     YNREFLAQGKTSQVLLHAESDDFVHWEKSETALELEPQEGYDIRNWRDPFVVWDDEKE
                     EYLLILGARKGQDKHLQTGRLVEFTSKDLTNWEFKGDFWAPGLYTMFEMPELFKIGDW
                     WYLVYSEYSDGNKILYRMSQSLEGPWIKPKDEAFDGRAYYAGRTAYDGKRRVLFGWVP
                     TKDQENDKNNYLWGGTLVPHEVYQKEDFSLGVKPIDEVWNAFINLHDIADVEIETADT
                     REEVTLVKNSGTLFNLETTISFDENLSDFSLRLYKNLETDESYEYRFNLDEGRLTFDK
                     NPCYPWFQYMDKGLIRPISLKPNQSYKLKVIVDDNIMTLYIDGIALNARVHGCFGQAI
                     TITATNGSMTAINTKFSNEIKK"
     gene            4913..6382
                     /locus_tag="U2K71_002252"
     CDS             4913..6382
                     /locus_tag="U2K71_002252"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM7316284.1"
                     /translation="MDYKKIAEDVLENVGGKENISSATHCITRLRLILKDKSKANMEM
                     LEEIEGAKGVLFNSGQLQVIFGTGIIEKVYKEFVELTGAKEVSVSEMKDEGVNNSGNR
                     LQRAFKVFSDIFIPIIPAFIGAAMVLGIRSLITTPGMFGMDGSFADHNVFLNDLSKFL
                     GIIATTFDYLPVLVMYSATKRFGGNPILGLLVGFVMIHPGLANRNDFVMGTIKPDYWH
                     LFGLSIPAVAFQGGVFPAILTSWFLAKVEKITNKYSPQVLAYILVPTVTIVLANFALF
                     LVFGPLGNFIGNVLGGIIDFLYMKMGAFGAFVFAGALQPLVVTGTQHAIQGIEANLVA
                     TTGFNYIQPIWSVSIIAQGGGCIGMYLLFKKKSKDRDIAISSFIPTLVGITEPAIFAV
                     NLKYSIVPFVCSFIGAGFGGAFMKIFDVKAIGQGLTVLPGLTIANPVLPYIFGNLIAF
                     ILPIIFILAYDKYKVFIPEAKHRKFAEKTDSKKLPVQVK"
     gene            6392..6859
                     /locus_tag="U2K71_002253"
     CDS             6392..6859
                     /locus_tag="U2K71_002253"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289220.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM7316285.1"
                     /translation="MLNFFRKNEISVVAPATGVFEKLEKLSDPVFSKGMMGQGFAIDA
                     KNETIVSPIDGFVSSIFPTKHALGIKTKSGIEILVHVGIDTVELNGEGFDIKVQEGQK
                     VKAGDILMTINFVVFEKNHKTKDVIVVFPDYQDEIFIPKLEEVKQGQELFVLG"
     gene            6895..7767
                     /locus_tag="U2K71_002254"
     CDS             6895..7767
                     /locus_tag="U2K71_002254"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM7316286.1"
                     /translation="MVFGAIEAGGTKFVCAIGDKDLNIFEKVSFPTTTPKETMNLVLD
                     FFNHHKDDLEGIGVGSFGPIDIHCESKTYGFIKSTPKLAWQDFDFIGSLKKQLNIPTA
                     WTTDVNASCYGEYVSGNGRGLSSVVYYTVGTGIGGGAIQNGNFIEGFSHPEMGHMLVK
                     RHNKDNFSGTCPFHGDCLEGLAAGPAIERRSGKKGQEVTENDVTWEIEAYYIAQCAYN
                     TTLMLSPDVIIFGGGVMKQKHLKKKVQDKFIELINGYVAIPDVDNYIMTPKLLDNSGI
                     VGCLALARDVKEKS"
     gene            7848..10382
                     /locus_tag="U2K71_002255"
     CDS             7848..10382
                     /locus_tag="U2K71_002255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM7316287.1"
                     /translation="MKKKMKDEVLEYIKNNLKYYDFSAQDIAMKFCIKRNVASHYLNQ
                     LFSDGKLLKNDSVRPVMFKYNQQKPKDCFSKFIGADISLKSTIDKCKATVMYPPNGLP
                     LIIKGNSGVGKSFLASLIYQYALDRKVIHNDAKFVVVNCADYANNPELLSAVLFGYKK
                     GAFTGAEKDTAGMLSSANGGYLFLDEVHNLSAENQEKLFLLMDSQKYRKLGESVQWEY
                     ANVRLILATTEDKNTSLLATFRRRIPAEITLPDYNNRSTSEKIQLLFEFLKDEAITID
                     SKIFCSIELFTDLLSKTFEGNVGELRNEIKLLCAEGYLGNKRKSSIYLGDKLDSGFWI
                     DPEISIDLKKMFLKSLSEIDLIELFQNHISIEESMSQLRDRILKECAGSSEYNYLESD
                     EFIALRNYVVNKISPIIDSTGLCLLDEFLADISLFIMFIDVIEETSRNFLLSKFRKFR
                     MKNDKQKLLANEIWSSLEVEESKQELLLKWILFLVKKFYVKIPETHCLIVMHGKITAS
                     AIARETNKLLNTYVYEAFDMPIEGETSDLINLINNFCKSIDTTNGLILLVDMGSLEQM
                     YEKIESNVIGDLVILNNVSTALAIECGIQVCQKKPISHFYQMDFDSFQVKVQYYKGLS
                     QKKNVIVSCLSGEGISEKVKDILKRYLDNQVEILIFDFNALKKIAKEKDTMIFKNTIC
                     VLSTTEFYIQGIDCLNLENLINGNQTLEKLNKYMDSDSCEFCLNELVKLFTIEGASTK
                     LRFLDSKKVFNEIEKVLYLYEKYYDVKIPSFLRINLFLHLSGMIERIMIGDGITNEHL
                     KEGAQLFDTFLNVSKDFFSEIKDIYCIEIPKGEYELIYTIFEQTIF"
     gene            10538..11311
                     /locus_tag="U2K71_002256"
     CDS             10538..11311
                     /locus_tag="U2K71_002256"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387092.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7316288.1"
                     /translation="MAFWQTFLVVLYGFFINWDKEGPKIGTSQPVVAGFLTGLIMGDI
                     QTGLYIGGTLQLMTLGITSFGGASVPDYQTAALIGTYLAISTNQDASIGITLAIPVAM
                     LIVQLDVLKWSTNIFFQQKAEKFAQKEDYQKLEWMQYLASINTSFVSGIPVLLTVILG
                     PTLVGSAINYIPEWVTGGLKVAGGLLPAVGIGMLLKYLPTKDYFAYLLVGFVLAVYFQ
                     IPILGISLLGAAIALIVYKNRSNNTQVAPAATGGFDEDE"
     gene            11304..12095
                     /locus_tag="U2K71_002257"
     CDS             11304..12095
                     /locus_tag="U2K71_002257"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM7316289.1"
                     /translation="MNKKLTKIDKQVIFRWILNGATSLNYEKMEGLGYAYSMLPFIKD
                     TYKDDPEGMKQSVLAHLQFFNTTPYTAPYIIGMNVGIEKEEKNKSLEAVSALKTGLMG
                     PLAGIGDSVFVVIPWTIFGAIAANMALEGSFVGIILWILASILLRITAYPLYLAGKES
                     GASLLTQIETKLKSITDAVSILGLMVVGALIATVVKVNVGFEFKQGDLTMTGNEIFDQ
                     VMPGLLPACVVAIVYYLLGRKVKPVYVILLILVVSIALRALNILK"
     gene            12105..12596
                     /locus_tag="U2K71_002258"
     CDS             12105..12596
                     /locus_tag="U2K71_002258"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7316290.1"
                     /translation="MDEKMKGIIHVRIDDRMIHGQVATQWTNQLSANRIMVINNEVAN
                     DDVKKAVVRLAAPPNVRTSIITREVAVKNILSGKYEGQKVLIVAVSPIDVKFLIDNGL
                     PITSVNVGNLSRRNGTERIRPTINITEEEREAFKELISDGVEVTVIQTPRDSKEFLKD
                     FIY"
     gene            12614..13024
                     /locus_tag="U2K71_002259"
     CDS             12614..13024
                     /locus_tag="U2K71_002259"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM7316291.1"
                     /translation="MEFIITSHGNIAIGFVDTLKMFFGDNLNCHVVTLGDAGIEEFRE
                     NIEKIVSQVKNQEVMVFADLIGGTPFNEFAKRSEVFQNGFQLLGGMNLGILIEAVNLR
                     LQGKKSDDIIENLRNMNTIACLADMQRHTNEEDE"
     gene            13028..14428
                     /locus_tag="U2K71_002260"
     CDS             13028..14428
                     /locus_tag="U2K71_002260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-fructofuranosidase"
                     /protein_id="HEM7316292.1"
                     /translation="MQKIFYKPKDFWFGDCMPFYKDGTFYLFHQRDTRNPKPLTDPFG
                     WSLVTTTDFVKFEEYGEVIHRGGNDDIDQYIYAGSIVQGINEYLALYTGHNRKAKLDG
                     KTSEVLMIASSKDLIHWKKDGEAAKLVPQEGYDKNDWRDPFAIFDQDHGKYVLILGAR
                     KPASKKKPTGRLVYFESEDLKDWNFKGDYWANNEFNMIEMPDIFEIDGTWYLLFSEYC
                     EDKKTKYRVGAGLFSDWKSPVDEAFDGKAYYAARTVGTNETGRFLTGWVATKEGENDQ
                     NNWDWGGALVPHQIIQKTDKTLGVKLPEKIIENFKEITPFSSFKLEKEFGKKERVIVP
                     KIEDYFLFETTLTITSLTKEFALKLFVDSDTQEGYEFRISLINNQLTFGKTPNYPWPQ
                     MFDKGLQRPLFLEIGKEYELKLIVDDTIAVLYIDNVALSTRMYDKVGTALSMTVTEGA
                     IKVDKLDYSINYECGF"
     gene            14451..14825
                     /locus_tag="U2K71_002261"
     CDS             14451..14825
                     /locus_tag="U2K71_002261"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016179839.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM7316293.1"
                     /translation="MKLNGLQHIGIPTKDFGASKIFYESLGFSLINEEMNVGSKVGFF
                     ELNGTIIEIWEDKTTECRGAIDHLALDTDDIDEVFNSIQDLGYELLDKNIMELPFWEN
                     GIRYFNFYGPNHEIIEISQMKK"
BASE COUNT         5287 a         2135 c         3046 g         4762 t
ORIGIN      
        1 ttttttattt gtctcgctta attatacaaa cggcgtgata aaaaacgaga aatgaattat
       61 aggtttagac ctttatcatt tctcgtttta ttttattgta tcaaggctct atcataagtg
      121 gtaaaatcta cttttaaatc acatgtaatc attgataagt ccagtgtttt agcggataat
      181 cagcttttta tttatttttt cttataaatt tgataaatag aaattagtat agccctaaac
      241 aaattatttg tgttttattg tttagggcta tactatattg gtacaaggaa tagaaagagg
      301 tgtttatgtg gttgataaaa ttcagtttaa tcaagttata gaagaattca tcaaagtaaa
      361 taatttaatg acccatattc aaaaacgacc aatcagaata actaatgatc taaaactcag
      421 tacgagtatg attcatttga ttgatgtgat tggaaattat cctgaaacta gtattacgga
      481 attagctgat cgattaggcg ttaccaaagg agctgtatcc caacagattc ccactttaga
      541 gaaaatgaat cttataagca tttctcaaaa aaaaggaaat aagaaaacta aacttgtatc
      601 gttaacaaaa tatggtcgaa aggtgtacga atcacataat tatttacatg aagagcttta
      661 tagctcaata aatgaagcgt tagaatcatt ctcaccagac caaatttcaa ccattaaaca
      721 gattctagaa aaagtatctt atacaatcaa tgaatatcaa gtaagtctga caagaaagga
      781 agattgacat gaaaaaaatg atttttgaat cggctacact agactagaca gaaaaaataa
      841 ggtgtgtaaa ataaatgaaa acacactgga ggaattttta tgtcaagacg tcaacgtaga
      901 acctattcaa aagaattcaa acaacaagtc gtcgatcttt atctcgcagg aaagccccga
      961 gctgaaatca ttcgagatta tgagctgact acctcggcct ttgataagtg ggtcaagcaa
     1021 gcgcaaacta ctggctcgtt tcaggaaaaa gataacttga tccctgaaca agtagaacta
     1081 attgcattac gaaagaaaaa taaacagctt gaaatggaga atgacatttt aaagcaagcg
     1141 gcgctgatat tgggacgaaa agacaagtaa ttgataccaa taagcacaaa tactcaatat
     1201 cagcgatgtg taaagtgtta aacatctctt gccaaaccta ttattatcaa gccaaaccag
     1261 ttgaagatga atcagaactg gaagaactcg ttcaagaaga atttattcgc aatcgaaaag
     1321 cttatggtac caggaagctg aagcagtgct tggccaatct aggtcttcaa gtgagtcaac
     1381 gcagaatcgg ccgtatgatg aaacagcgtg gcttgcgttc aacatacaca ttggctcatt
     1441 tcaaattcca ttcttctact tgtaacgaag ccaagaccgc caatattcta gacggaacct
     1501 tcctacagga aaagtcatta gaagcgattg taaccgacct tacctatgtt cgtgttggga
     1561 agaaatggca gtatatctgc ctgattcttg atttgtttaa tcgagaaatc atcggctatt
     1621 catgcgggga gaagaaagat gcgacgttag tcaaggaagc ttttgcaaag attccgtact
     1681 cgttgaataa tgtacaactt tttcacacgg atcgtggaaa agaattcgat aaccagacga
     1741 ttgacgaaat tttatgggcc tttaatatca cgcgttcctt aagcaaaaaa ggctgcccct
     1801 atgataacgc cgttgcagaa tccacctata aatctgtcaa agtagaattc gttcatcaat
     1861 accgctttga gacattggct aatttgcgat tagagctatt tgattacgtc cattggtgga
     1921 ataccatccg gctgcacggc acgttaggct atgagacacc gatcagtttt cgacagcaga
     1981 gattggcgca gcgaatcctt gataatgagc tcggatgtga taccggtggc gaggcacctt
     2041 aattatttga atgagcttct gccttagaga atcagattcg aggaggctcg ttgtcaaggg
     2101 cagtcggaac accagcagca agcttacgca ataccttata atttttgtca aaaaaagtgt
     2161 tgccattcca catatatctt taaacagcta ttgccagata aaaataaatg atattatata
     2221 tataaatact tatccgaaaa ggggaatgag agagtcaagg ttcttggaat gtcgaatagt
     2281 cgagtaggta gattttctat ctacaacctt gttgtttaaa aatgaaaaaa attggaatta
     2341 gagaagttgc gaaagaggca aatgtttctc ctacgactgt gtcgagggta ttgaatgatc
     2401 gtggatattt gagtgaggaa acaaaaagaa aagtttttga tgccatgaaa aaattagaat
     2461 actatcctaa tgaactggcg agagcacttt ttaaaaacga aacatatacc gtaggtttga
     2521 tttttcctac aattactaat ccatttcacg ctgagttaat tcaagatatc gagttcgagc
     2581 tttccagcag gggatataaa gtgttgctgt gcaatagctt aaacaatcca acaaaagaaa
     2641 aagagtactt gacgatgttg agaaagaatc aggttgatgg aattatagta ggaacgcaca
     2701 ataaagatat tgcggattat gatattccag ggctacctat tgtagcgatt gatagaagtc
     2761 ttggtgaaaa tacagttaca gtatcttgtg ataattacag gggtggggaa ttagctactc
     2821 aactacttgt aaatcatggc tgtcagaata tactgtgtat tcggggagat agtaagataa
     2881 agctacccgc gaatagtagg actttagcat ataaagatgt tttgaataaa ttcatgctgg
     2941 aaccgttagt cctagaagta ccatttgtaa aagaaatcaa tgaaaaaaat gaaataatta
     3001 agacatatct ggaaactcat ccaaatattg acggggtttt tgcaggagat gatttattgg
     3061 cttctttggc gattaacaac ttaggaagtt cgggcaaaag agttccaaaa gaggttaaag
     3121 tgattggatt tgatggagct caacaaacat tgatttataa tccagaatta tcaactattc
     3181 aacagcctat agatcaaata tctaaagtag cagtaaaaaa attattgaat atgattaagg
     3241 gcgaaaagga gacggatgaa ctttcacttc cagtaaagct cataaaaaaa aatacagcat
     3301 aagttaaatc ggcgaaaaat tccttcgtcg atttaaaaat atttttatga aacgggttga
     3361 catatgaaac gggttgacat atattagttg tgaagataaa aaccttttca ctggaggaag
     3421 aaaatggaga aattattaat taaaagcaat aatcatcaac tttactacca acctaaggat
     3481 gtttgggtcg gagatgttat gccttacggg aaagaaggaa aattctttat ttatcatcaa
     3541 cgtgatacta gaaatccagt tccttttgga gaaccgtttg gctgggcttt agcaactacg
     3601 aaagattttg ttcattttga agactttggg gaatctcttt taagagggga tgacaatgag
     3661 agagaccagt ttatctacgc tggttctgta tttgaggcaa atggaaaggt tcacgcattt
     3721 tatacagggt ataatcgtga attcttagcg cagggaaaga catctcaagt gttgcttcat
     3781 gctgagagtg atgattttgt tcattgggaa aaatcagaaa cagcattgga gttagaacca
     3841 caagaagggt atgatattcg aaactggaga gatccatttg tcgtttggga tgatgaaaaa
     3901 gaagaatatt tgctaatttt aggggcgcga aagggtcagg ataaacatct acaaaccgga
     3961 cgtttagtgg aatttacatc aaaagattta actaattggg aattcaaagg tgatttttgg
     4021 gctccgggat tgtataccat gtttgaaatg ccagaacttt tcaagattgg tgactggtgg
     4081 tatcttgtct attcagaata ctcagatgga aataaaatac tctatcgaat gagtcaaagt
     4141 ttggaaggtc cttggattaa gcctaaagat gaagcttttg atggacgggc ttattatgca
     4201 ggtagaacag cttatgatgg aaaacgtcga gtcctatttg gatgggtacc aacaaaagat
     4261 caagaaaatg ataaaaacaa ttatttatgg ggaggtactt tggttcctca tgaagtctat
     4321 caaaaagagg atttttcact tggggtaaaa cccatagacg aagtgtggaa tgcattcata
     4381 aatttacatg atattgccga tgtagagata gaaacagcag acacaagaga agaagtaacg
     4441 cttgtcaaaa atagtggaac tttgtttaat ttagaaacaa caatttcatt tgatgagaat
     4501 cttagtgact tttcactgag attatataaa aaccttgaga cagatgaatc ttacgaatat
     4561 cgcttcaatt tagatgaggg gcgtcttaca tttgataaaa acccttgtta cccttggttt
     4621 caatatatgg ataaaggact tattagacct atatctttaa agcctaacca atcttataaa
     4681 ctaaaagtga ttgttgatga caatataatg acactttata ttgatggaat cgctcttaac
     4741 gctcgtgtac atggttgttt tggtcaagca attactatta cagcaacaaa tggatcaatg
     4801 actgcaataa ataccaagtt ttcaaatgaa ataaagaaat aataaacgtc aatcatgtgg
     4861 caggtctgaa aatcaaacat gacctgtcac atttaaaagg gaggataaat gaatggacta
     4921 caaaaaaatt gccgaagatg tattggaaaa cgttggagga aaagagaata tctcaagcgc
     4981 tacacactgc attacccgtt tacgtttaat tcttaaagac aaaagcaaag cgaatatgga
     5041 aatgcttgag gaaatagaag gtgcaaaagg tgttcttttc aatagtggac agttacaagt
     5101 aattttcgga acaggtataa ttgaaaaggt ttacaaagag ttcgttgaat tgacaggagc
     5161 taaggaagta tccgtttctg aaatgaaaga cgaaggagtc aataattcag gaaatagact
     5221 tcaacgagct tttaaagttt tctcggatat ctttattccg atcattccag cttttattgg
     5281 ggcagcaatg gttttaggaa ttcgttcgtt gattaccact cctggaatgt ttggaatgga
     5341 tggctcattc gctgatcaca atgtattcct aaatgattta tccaaatttc tgggtattat
     5401 tgcaaccaca tttgattatt taccagtttt agttatgtat tctgcaacaa aacgttttgg
     5461 cggaaaccct attttaggat tacttgtagg atttgttatg attcatccag gactagccaa
     5521 tcggaatgat ttcgtcatgg gaactataaa gcctgattat tggcatttat ttggattaag
     5581 cattccagca gttgcgtttc aagggggtgt ttttccagca attttgactt cttggttttt
     5641 agcaaaagtt gagaaaatta caaataaata ttctcctcaa gtacttgctt acattttagt
     5701 cccaactgtg acaattgttc ttgccaactt tgcactattt ttagtgtttg gtccattggg
     5761 gaattttatc ggtaatgttt taggtggaat cattgatttc ctatatatga agatgggtgc
     5821 ctttggtgct tttgtttttg caggcgcgtt acaaccatta gttgtaacag gaacacaaca
     5881 tgctattcaa ggtattgagg caaacttagt cgcaacaact ggattcaatt atatccaacc
     5941 tatttggtca gtttcaatta ttgcccaagg tggcggttgt attggtatgt atttactctt
     6001 taagaaaaaa tctaaagata gagatattgc gatttcaagc tttattccaa cactggtagg
     6061 tattactgaa ccggctatct ttgctgttaa tttgaaatat tcaatcgttc cttttgtttg
     6121 ctcgtttatt ggtgctggtt ttggtggagc atttatgaaa atttttgatg taaaagccat
     6181 tggacaagga ttgactgtat tgccaggtct gactattgct aatccagttc ttccttatat
     6241 atttggtaat ctcatagcat tcattttacc aatcattttt attctcgctt atgataaata
     6301 taaagtgttt atccctgaag ctaaacaccg taaatttgca gaaaaaactg actcaaaaaa
     6361 attgccagtt caagtaaagt gagatgagag catgttaaat ttttttagaa aaaatgaaat
     6421 aagcgttgta gcacctgcaa ccggtgtttt tgaaaaactg gagaaattaa gtgatccagt
     6481 cttttcaaaa ggaatgatgg gacaaggatt tgcaattgat gcaaaaaacg aaacgattgt
     6541 atctccaatc gatggttttg tttcaagtat ttttcctaca aaacatgcct taggaataaa
     6601 aacaaaatca ggaattgaga tacttgttca tgtaggaata gatacagttg agctaaatgg
     6661 tgagggattt gatattaaag ttcaagaagg acaaaaagtt aaggctgggg atattcttat
     6721 gaccattaat tttgtagttt ttgaaaagaa tcacaagact aaagatgtca ttgttgtttt
     6781 tccagactac caagacgaaa ttttcattcc gaaacttgaa gaagtaaaac aaggacaaga
     6841 attgtttgtt cttggctaat gataagtaat taataataga atcgaggaaa atgtatggtt
     6901 tttggggcta ttgaagctgg tggaacaaaa tttgtttgtg ccattggaga taaagatttg
     6961 aacatatttg aaaaagtcag ctttcccacg actacaccca aagaaacaat gaatttagtt
     7021 ttagattttt ttaatcatca taaagatgat ttagaaggta tcggagtggg atcgttcggt
     7081 cctattgata ttcattgtga atctaaaacg tatggcttca ttaaatcaac gccaaaactt
     7141 gcttggcagg attttgattt tattggaagt ttaaaaaagc aacttaatat tccaactgct
     7201 tggacgactg atgtaaatgc ctcttgctat ggtgaatatg tttctggaaa tggcagaggc
     7261 ctttcaagtg ttgtttacta taccgtagga acgggcatag gaggtggcgc tatccaaaac
     7321 ggaaacttca ttgaaggttt tagtcatcca gaaatgggac atatgcttgt aaaacgacac
     7381 aataaagaca acttttctgg gacatgtcca ttccacggag actgtttaga gggcttagct
     7441 gctggtccgg caatcgaaag aagatcaggg aaaaagggac aagaagtgac tgaaaatgac
     7501 gttacttggg aaatagaagc ttattacatt gctcagtgtg catataatac aacattgatg
     7561 ttatcacctg atgtcattat atttggtggc ggagtaatga aacaaaaaca cttaaaaaag
     7621 aaagtacaag ataagtttat tgagttgatc aacggatatg tcgctattcc cgatgttgac
     7681 aattatatta tgacaccaaa attgttagat aattctggta ttgtaggttg tttagcttta
     7741 gcaagagacg ttaaagaaaa atcttaattt ggaaaaaaga tcgtctcgac tatttgtttg
     7801 gttgatgcga tctttttttc aattagtaat tgaagggacg cctaaaagtg aaaaaaaaaa
     7861 tgaaagatga agtattagag tatatcaaaa ataatttaaa gtattacgat ttttctgctc
     7921 aagatattgc aatgaaattt tgtattaaaa gaaatgtcgc ttcccactat ttgaatcagt
     7981 tattttcaga tggaaaatta ctaaagaatg attctgttag accagtaatg ttcaagtata
     8041 atcaacaaaa gccaaaagac tgcttttcta agtttattgg agcagacata tctttgaaat
     8101 ccactataga taaatgcaaa gctactgtaa tgtatccccc taatggcctc ccattaataa
     8161 taaaaggaaa cagtggcgtt ggaaaaagtt ttttagcaag tttgatctat cagtatgctc
     8221 ttgatagaaa agtaattcat aatgacgcaa aatttgttgt tgtgaattgt gcagattatg
     8281 caaataatcc agagctattg tcagcagttt tatttggata taaaaaagga gcctttactg
     8341 gggctgaaaa agatactgca gggatgctat cttctgctaa tggaggatac ctttttttag
     8401 atgaggttca caatctaagt gcagaaaacc aagagaaatt gtttcttctt atggattctc
     8461 aaaagtatcg taaacttggt gaaagtgtgc aatgggagta tgcaaatgtt cgccttatct
     8521 tagccacaac agaagataaa aatacctctt tgttagcaac ttttagaaga cgcatcccag
     8581 ctgaaatcac tctgccagat tacaataata gaagtacatc tgaaaaaatt caattattat
     8641 ttgaatttct taaagatgaa gcaattacga ttgattcaaa aatattttgt tcgattgaac
     8701 tttttactga tcttttatcg aaaacatttg aaggaaatgt tggagaattg aggaatgaaa
     8761 tcaagcttct ttgtgcagaa ggttatttag gaaataagag aaagtcttca atttatttag
     8821 gggacaaact agacagcggt ttttggatag atccagaaat tagtatagat ttaaaaaaaa
     8881 tgtttcttaa atcactttct gaaattgatt tgattgaact ttttcaaaat cacatttcaa
     8941 ttgaagaaag catgagtcaa ttaagagata gaatacttaa agagtgtgca ggttcttctg
     9001 agtataatta tttagaaagt gacgaattta tagcattaag aaactatgtt gttaataaaa
     9061 tatcaccaat tattgattcg acaggtctat gtttattaga tgaattcttg gcggatatca
     9121 gtctatttat aatgtttatt gatgtaattg aagaaacatc aagaaatttt ttgctgtcta
     9181 agttcagaaa atttcgtatg aaaaatgata aacaaaaatt attggcaaat gagatttgga
     9241 gtagtttaga agtcgaagaa agtaaacaag aacttttgtt gaaatggata ctttttcttg
     9301 taaagaagtt ctatgtgaaa ataccagaaa cccattgtct gatagttatg cacggtaaaa
     9361 taacagcttc tgcgatcgca agggaaacaa ataagttgct gaatacatat gtttatgagg
     9421 ctttcgatat gccaattgaa ggtgagacta gtgacttaat taacctaatc aacaattttt
     9481 gtaaaagtat agatacgacg aatggactca ttctgctagt tgatatggga tcattggaac
     9541 aaatgtatga aaaaattgaa tccaatgtaa taggtgattt ggtaatttta aacaatgttt
     9601 ctacagcttt agcgattgaa tgtgggattc aagtttgcca aaaaaagcca attagtcatt
     9661 tttaccagat ggattttgat tcgtttcaag ttaaagttca gtattataaa ggattatcac
     9721 aaaagaaaaa tgtaatagta tcttgtctct ctggtgaggg gatttctgaa aaagttaagg
     9781 atattttaaa acggtatctc gataatcaag tggagatttt gatttttgat tttaacgcat
     9841 taaagaaaat agcaaaggaa aaagatacta tgatttttaa gaatacaatt tgtgttcttt
     9901 caaccactga attttacatt caaggaatcg attgtttaaa tttagaaaat ctaattaatg
     9961 ggaatcaaac cttagaaaaa ttaaataagt atatggactc ggatagctgt gagttctgct
    10021 taaatgaatt agttaaactc tttactattg aaggcgctag tactaagctt cgctttttag
    10081 attccaaaaa agtattcaat gagatagaaa aagtactata tttgtatgaa aaatattacg
    10141 atgtcaaaat cccaagtttt ctgagaatta atctatttct acatctttct ggaatgattg
    10201 aacgaattat gattggtgat ggaataacaa atgaacattt gaaagaggga gcgcaactat
    10261 ttgatacttt tttaaatgta tcaaaagatt ttttttcaga aatcaaagat atttactgta
    10321 ttgaaatacc caaaggagag tatgagttaa tctatacaat ttttgagcag actattttct
    10381 aaatagcata ctagcgtcta aaaaaatgaa agggtgtcca aagtatattt agacgctcat
    10441 ttaattaata tttactgtat tttcccgctt tattggataa tgttagttgg cacagtaatt
    10501 gcttatataa aagtataaga agaaaggaga gaacaaaatg gcgttttggc aaacattttt
    10561 agttgtgttg tatggatttt tcattaactg ggataaggaa gggcccaaaa tcggaacctc
    10621 ccaacctgtc gttgcaggtt tcttgaccgg acttattatg ggagatattc aaactggact
    10681 ttatatcggt gggactttgc aattaatgac actaggtatt actagttttg gtggtgcatc
    10741 agtaccggat tatcaaacag cagcattgat cggtacttat ttagcaatct caactaacca
    10801 ggatgcttct ataggaataa ctttagcaat accggtagca atgttaattg tacaactaga
    10861 tgttctaaag tggtcaacaa acattttttt tcaacaaaag gcagaaaaat tcgcacaaaa
    10921 agaagattat caaaaattag agtggatgca atatttggca tcaattaata caagctttgt
    10981 atctggaatt cctgttttgt taactgtgat attaggccca acgttagtgg gaagtgcaat
    11041 caattatatt cctgaatggg ttaccggtgg tttaaaggta gcagggggac tattacctgc
    11101 tgttgggata ggaatgctac ttaaatattt acctacaaaa gattactttg cctatttgtt
    11161 agttggtttc gttttggcag tttatttcca aataccaatt ttagggattt cgttacttgg
    11221 cgctgcaatc gcactaatcg tatataaaaa tagatcaaat aatactcaag tagcaccagc
    11281 tgcaacagga ggttttgacg aagatgaata agaaactgac aaaaatcgac aaacaagtaa
    11341 ttttccggtg gattctcaat ggtgcaactt cattaaatta tgaaaaaatg gaagggttgg
    11401 gttatgcgta ttctatgctt ccttttatca aggacacata taaagatgat cctgaaggaa
    11461 tgaaacaatc cgttttagct cacttacaat tttttaacac tacgccgtat acagcgcctt
    11521 acattattgg tatgaatgtt ggaattgaaa aagaagaaaa aaataagtct ttggaagcag
    11581 taagtgctct gaaaacaggt ctaatgggtc ctttagcggg aattggagat agtgtatttg
    11641 tagttattcc atggactatt tttggagcaa ttgcagctaa tatggcattg gaaggtagtt
    11701 ttgttggaat tattttgtgg atactggcat caattttatt aaggattact gcttatcctt
    11761 tataccttgc tgggaaagaa tcgggtgcga gcctactaac tcaaattgaa acaaaattaa
    11821 agtctattac tgatgcagta tcaattttag ggttaatggt ggtcggtgca ctcattgcca
    11881 cagttgtaaa agtgaatgtt ggctttgaat ttaaacaagg cgacttaaca atgaccggaa
    11941 atgaaatctt tgatcaagtt atgcctggtt tattacctgc atgtgtagta gcaattgtgt
    12001 attatttgtt aggacgaaag gttaaaccag tttatgtaat tctattaatc ttagtagttt
    12061 ctattgcgtt aagagcgtta aatattttga agtaggtgtt gaatatggat gaaaaaatga
    12121 aaggtattat acatgttcgt attgatgatc gaatgattca tggacaagta gcaacacaat
    12181 ggacaaatca attgagtgct aacagaatta tggttattaa taatgaagta gccaatgatg
    12241 acgtaaaaaa agctgtagtt cgcctcgctg caccacctaa tgttagaact tcaataatta
    12301 caagggaagt agcagttaaa aatattcttt ctggtaaata tgaaggacaa aaagttctta
    12361 ttgttgcagt ttctccaatc gatgttaaat ttttgattga taacggttta ccaataacaa
    12421 gtgtaaacgt aggaaattta tcaagaagga atggaacaga acgcatccgt ccgacgataa
    12481 atattactga agaagagaga gaggctttta aagaactaat tagtgatgga gttgaagtga
    12541 cagtgataca aactcctaga gattcaaaag aatttttaaa ggattttatt tattaagaac
    12601 agaggatgag tggatggagt ttataattac atctcatgga aatattgcca ttggatttgt
    12661 agatacatta aaaatgtttt ttggcgataa tttaaattgt catgtggtaa cacttggaga
    12721 tgcaggcatt gaagaattcc gtgaaaacat tgaaaaaata gtttctcaag taaaaaatca
    12781 agaagttatg gtatttgctg acttgattgg tggaacacct ttcaacgaat ttgctaaaag
    12841 aagtgaagta tttcaaaatg ggtttcaact tcttggtggc atgaatttag gaattttgat
    12901 tgaggctgtc aatttgagat tacaaggaaa aaaaagcgat gatatcatcg aaaatctaag
    12961 aaatatgaat acaattgcgt gtcttgcaga tatgcaaaga catactaacg aagaggatga
    13021 gtgataaatg caaaaaattt tttacaagcc taaagatttt tggtttgggg attgcatgcc
    13081 tttttataaa gatggaactt tttatctatt tcatcaacga gatacgagaa atcctaaacc
    13141 tttaactgat ccttttgggt ggtcactagt tacaaccact gattttgtga aatttgaaga
    13201 atacggggaa gtaattcatc gtggtggcaa cgatgacatt gatcaataca tttacgctgg
    13261 aagtattgtc caaggaatca atgagtattt agctttgtat actggacata atcgcaaagc
    13321 taagttggat ggaaaaactt cagaagtgtt aatgattgca agttctaaag atttaattca
    13381 ttggaaaaag gatggcgaag cagcaaaact tgttcctcaa gaaggatatg ataagaatga
    13441 ttggcgcgat ccttttgcaa tttttgatca agatcatgga aaatacgttt tgatactagg
    13501 agctagaaaa ccagcaagta agaaaaagcc gacggggaga ttggtctatt ttgaatccga
    13561 agatttaaaa gattggaatt tcaaaggaga ctactgggct aataatgaat ttaacatgat
    13621 tgaaatgcct gatatatttg aaattgatgg aacgtggtat ttactttttt cagagtattg
    13681 tgaagataaa aagactaaat atcgagtcgg agcaggatta ttttcagatt ggaaatcacc
    13741 agtagacgaa gctttcgacg gaaaagccta ttatgcagca agaacagtcg gaactaatga
    13801 aactggccgt tttctaactg gctgggtggc aacaaaagaa ggggagaacg atcaaaataa
    13861 ctgggattgg ggcggtgcac ttgttccaca tcaaattatt caaaagacag acaaaactct
    13921 tggtgttaaa ctgcctgaaa aaataattga gaattttaaa gaaatcactc cattttcaag
    13981 ttttaagtta gagaaagaat ttggaaaaaa agaaagagta attgttccta aaatcgaaga
    14041 ttattttcta tttgaaacaa ctttgactat tacatcgtta acaaaggagt ttgctttaaa
    14101 attatttgta gatagtgata ctcaagaagg atacgaattt cgtatttcat tgattaataa
    14161 tcagcttact ttcggaaaaa ctccgaacta tccttggcct caaatgtttg ataaggggct
    14221 gcaacgacca ctatttttgg aaataggcaa agaatatgaa ctaaagttaa ttgttgatga
    14281 tacgatagct gttttatata ttgataatgt tgctttaagt actagaatgt atgataaagt
    14341 cggaacagca ttaagtatga ctgttacgga gggagcaatc aaggtagata aactagacta
    14401 ttcaataaat tatgaatgtg gattttaaaa caattgaagg gatgatgtaa gtgaaattaa
    14461 atggtttaca acatattgga attccaacaa aagattttgg agcttcaaaa atcttttatg
    14521 aatccttagg gtttagtctg attaatgaag aaatgaatgt gggaagtaaa gtcggctttt
    14581 tcgagttgaa tggaacgata atagaaatat gggaagataa aactactgag tgtagaggag
    14641 ccattgatca tctagcatta gatacggatg acatagatga ggtattcaat tcaattcaag
    14701 atttaggata tgaattatta gataaaaata ttatggaatt accgttctgg gaaaatggaa
    14761 tccgttactt taatttctac ggaccaaatc atgagattat agagataagc caaatgaaga
    14821 aataaataga taacgaagtg ctctaagttg gtatgaacga aaagtacttc attttttggt
    14881 atcagcattt caatagttta atgagaattt actatttcaa attaattcaa agtgacagta
    14941 ttttttactt atttggtttc aaattttgta atccttccta tttattggaa tacactccac
    15001 attttgtata caagtcatat catgttttag caaagctctt accaagcaaa gttataatga
    15061 tgttttccaa agtttgatct ttcgatggag gctaacacat attttttgca ggacagccaa
    15121 aattagatgt atagaataga gtctgtgagg cgtatagaca gtttattgtc tgtatgcctt
    15181 tttttatttt gaaaaatttt tagtattttt cgaaaaatgc tcactcctag
//
LOCUS       DASHHB010000061        14566 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.063, whole genome shotgun sequence.
ACCESSION   DASHHB010000061 DASHHB010000000
VERSION     DASHHB010000061.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14566)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14566)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14566
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.063"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..616
                     /locus_tag="U2K71_002262"
     CDS             <1..616
                     /locus_tag="U2K71_002262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEM7316294.1"
                     /translation="FVFAPLEGEDLASFLVGAAIMMIGMTLFLFGADYSMMEVGDLVG
                     KYMIKKKSLTVLVSLGFAIGIVITIAEPSVQVLGQQVYDISDGEIGRVLLIGIVSVGT
                     GVFLAFALLRVVFKLSYYQLMAIGYIGVLIASFFTSSEFMPIAFDSGGVTTGPVTVPF
                     ILALAGGMTSMIKQKKNENDSFGMVGIASLGPILAVMILGVIFQ"
     gene            613..1386
                     /locus_tag="U2K71_002263"
     CDS             613..1386
                     /locus_tag="U2K71_002263"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1538 domain-containing protein"
                     /protein_id="HEM7316295.1"
                     /translation="MSFWQQVIDGIEIVTMEVILAVAPIVILFVLMNIISFRMRKKRF
                     FDIMKGFLITTIGLILFLHGVNMAYVPVGQHLGSAIAGLPYNYILVPLGFLMGFLVGF
                     AEPAIHVMIKQVEELSEGRIRSKIMLMVISIGIGAAVGLSMWRLLVGFSLYYFLIPGY
                     GLAFILGRKVDKIFLAMAFDNGGVATGPMCSTFILSMSVAIAAQIEGRDPIIDGFGVV
                     AMIALTPILSTLVLGYIYKRKDEKDRIEKERLQKLGESK"
     gene            1383..1781
                     /locus_tag="U2K71_002264"
     CDS             1383..1781
                     /locus_tag="U2K71_002264"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HEM7316296.1"
                     /translation="MKDLVALNLHMIVTIVDGGMGEDVIDFSKEAGAAGGTILHGRGS
                     GVHDSGKFLGLDISPEKDIVLTLVPDSLTNKVMDAIGQGINIDIPGNGICFSIDVDKV
                     IGITKIHQYREIIGMQELLDENNDREKSES"
     gene            1966..2637
                     /locus_tag="U2K71_002265"
     CDS             1966..2637
                     /locus_tag="U2K71_002265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316297.1"
                     /translation="MNFLVVLKDTLIERFTFLYVQLQENKTLLHFSPEFHLEGLTLGE
                     IYQAEGLSAVLHFFKVELELPVKDYIELSLESWDRAVVELFPEGLMIDTNDGKIHLTA
                     AELQKQMRYQIDDENSFSIFRRQQKILKSFLKVISKKRNLIKTTQLLKKYPGLLHTSI
                     QFPQLITMIHRFIRMQQLPSKKLFLPLTDTFRVVNREDKKKVIVIDFTRNRNVLHQVA
                     MEKAN"
     gene            2696..3136
                     /locus_tag="U2K71_002266"
     CDS             2696..3136
                     /locus_tag="U2K71_002266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316298.1"
                     /translation="MMQTWLERLIRFQQNYHKLMQGRYAKFDQLNRGLLIAALVFSLL
                     SRWLPYRIGQLLFIVFFGWMIFRFLSKKIYPRLNENQRYLKYLDRVKEKYHQIKNKKQ
                     VKMDRKSYTFFECPNCHQKQRAPKGKGRIRVTCKTCGIKFETNV"
     gene            3148..3816
                     /locus_tag="U2K71_002267"
     CDS             3148..3816
                     /locus_tag="U2K71_002267"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-bisphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HEM7316299.1"
                     /translation="MKLVLLRHGESEANFENYWTGWLDVALTEKGKEQAAVAGQKMLE
                     AGLQFDAVYTSVLKRAILTGQVALEEMGQLWLPIIKTWRLNERHYGDLVGKNKDKMKE
                     IYGKDQVKTWRRGYTEVPPLTAENRFDRRYDHLDPHLIPYGESLEMTVNRIIPLWQDH
                     LAPKLKDRQDLLVVGHGNSIRALTKYLEDVPEDQMDTIDIPNAQPIQYTLADDLTILD
                     KKIL"
     gene            3880..4503
                     /locus_tag="U2K71_002268"
     CDS             3880..4503
                     /locus_tag="U2K71_002268"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HEM7316300.1"
                     /translation="MKNYIWDFDGTLFDTYPAMVDGAWQALKDFGISMDKKEIYFKMK
                     KYSTSYLINESNLNAREFNELFHRYEKESTEVSRPFPETKQVLEMLKDNGGRHFILTH
                     RLTESTWGLLKEHRLAHLIEEVVGIDQDFPRKPDPASLNYLIDTFHLERTDTMMIGDR
                     RLDIEAGKNAGVATCLYDIDHFLGEIPADYVVGNLNEILTLQSIIKK"
     gene            4559..4945
                     /locus_tag="U2K71_002269"
     CDS             4559..4945
                     /locus_tag="U2K71_002269"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1149 family protein"
                     /protein_id="HEM7316301.1"
                     /translation="MEIRRQKEVVEAFHYDMRLPNQEVETDLKVGFSPLKETIENYPK
                     EHSIVGARLEFRLVFDTYVLSGSVSQINHILNRKIEGQNDVTQEEVDELVAPLFDIVR
                     RMSYEVTEIALDQPGVKLNFQSEKQE"
     gene            5158..6549
                     /locus_tag="U2K71_002270"
     CDS             5158..6549
                     /locus_tag="U2K71_002270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-cystine transporter"
                     /protein_id="HEM7316302.1"
                     /translation="MTTLITVLVVLVFAALLYVFYRMQKRHTKFSTRVFTALGVGIVF
                     GGLLQLIFGVDDKVVTQSMEWIGIVGQGYVSFLQMLVMPLVFISIIGAFTKMKVSEKL
                     GKISATVLTSLLGTTAIAALIGIGSTMLFGLQGAKFTQGAAETARISELATRQETVEN
                     LSIPQQILSFIPKNVFADLAGSRATSTIGVVIFAAFVGIAYLGVKRKAPKEAAFFSDL
                     VDSLYKIIMRIVTLVLRLTPYGVFALMTNVLATSDFAAIVNLGKFVVASYVALLAVLI
                     VHSLILIGAKVNPINYFKKAFPVLSFAFTSRSSAGALPLNIETQTKALGVDDATANFS
                     ASFGLSIGQNGCAGVYPAMLATIVAPAAGVNVFSLEFILMLVAIVTISSFGVAGVGGG
                     ATFASLIVLGAMNLPVAIVGLVISVEPLIDMARTAVNVSGSMVAGIVTSARIKDLDRD
                     VINDDSKVIDVNA"
     gene            7160..7528
                     /locus_tag="U2K71_002271"
     CDS             7160..7528
                     /locus_tag="U2K71_002271"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactoylglutathione lyase"
                     /protein_id="HEM7316303.1"
                     /translation="MFTNEIKIMLYVDDVEKNATFWRAVGFAEKDRQEMDGTLIIEVG
                     VEDSQTSFLLYDRAFVESHSPGTATSAPSLMFYSKDIFGLYKKMQTQSVTLGDMVQLG
                     EEYVFNFADPDGNYFAVTGK"
     gene            7708..8649
                     /locus_tag="U2K71_002272"
     CDS             7708..8649
                     /locus_tag="U2K71_002272"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-related sulfurtransferase"
                     /protein_id="HEM7316304.1"
                     /translation="MDYQVLLYYNYTKIEEPEEFAKNHLAFCKSLDLKGRILVAEEGI
                     NGTVSGTVENTTAYMKAMKADPRFADTMFKIDPSEGHAFKKMFVRYRPELVSLKLEED
                     IDPNQVTGAYLSPQEFKEAILDEQTVVIDARNDYEYDLGHFRGAVRPDIRSFRELPDW
                     IREHKEEFMEKRVVTYCTGGIRCEKFSGWLVREGFKDVGQLHGGIATYGKDPEVQGEL
                     WDGKMYVFDERIAVDINQVDKQIVGRDWFDGTPCERYINCSNPECNRQILASIENEEK
                     HLGACSIECAKHPRNRYVQDHGISPEEAELRFTALIK"
     gene            8718..9449
                     /locus_tag="U2K71_002273"
     CDS             8718..9449
                     /locus_tag="U2K71_002273"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine/threonine protein phosphatase"
                     /protein_id="HEM7316305.1"
                     /translation="MKSLIYAIGDVHGKHELFEKLIHYYDPKIHQLVLLGDLNDRGPR
                     TKECFLLGKQLVEETGAIYLRGNHEEYFLQFLQSPEDWYAPYMRNGGKETFESLIHPG
                     ASEEYSPTEISMMIQSRYSELIKFLVQRPLYYEWENYLFVHAGVDLTKKDWRKTSLRD
                     FIWIREPFHEGKNNTGKTIVFGHTITPMLHGDMQTTDLWIQDHKIGMDGGAVFGGSLH
                     GVVFDKEGILQDIEYQNNEGPWHGI"
     gene            9593..11773
                     /locus_tag="U2K71_002274"
     CDS             9593..11773
                     /locus_tag="U2K71_002274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding transcriptional accessory protein"
                     /protein_id="HEM7316306.1"
                     /translation="MTENLNQTIIQLVQKELSDYRPKQLTTVLNLLNEGNTVPFIARY
                     RKEMTGSLDEVQIREIEERYAYLENLEKRKTEVIRLIDEQGKLTPELEAEITQAVKMQ
                     QVEDLYRPYKQKRRTKATIAKEKGLEPLAKWLMQLTDGEVQSEAEKYIDKEKEVSSAE
                     EALHGAHEIIAEQVSDNAKFRTWIRSYTYNKGMYVSNVKDEQADEKGVYEMYYDFAEP
                     VHKMVSHRILATNRGEKEDILKVFFQVDEAAILAYLDRQIVKNPASPSSSFVREAYQD
                     SYKRFIQPAIERELRNELTEKADEQAIAIFGENLRNLLLQPPLKGKVVLGFDPAYRTG
                     CKLAVVDATGKVLAIEVIYPHKPAAQAKREAAGPAFIQLINKYQVDMVAIGNGTASRE
                     SELFVAEQLKSADHKTYYAIVNEAGASVYSASEIARKEFPHLQVEERSAVSIARRLQD
                     PLAELVKIDPKAVGVGQYQHDVSQKRLAEQLDFVVETAVNQVGVDVNTASPQLLQHIS
                     GLNKTTAQNIVIYREENGEFTARTQLKKVPRLGPKAYEQAIGFLRVPGGKNILDNTGI
                     HPESYSIAKEILTSVQLSEKELGTEEAVEKLTRLSVEKLAESLSVGEETLADILAGLT
                     QPGRDMRDEMPAPLLRTDVLSMEDLKPGMELTGTVRNVIDFGAFVDIGVKQDGLVHIS
                     KLSKKFVKHPTDVVSVGDIVTVWIEQVDTKKGRISLTMLSPYEE"
     gene            11793..12275
                     /locus_tag="U2K71_002275"
     CDS             11793..12275
                     /locus_tag="U2K71_002275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SprT family protein"
                     /protein_id="HEM7316307.1"
                     /translation="MTQEELQQLVEEISLKSFQLPFKHQAMFNPRLRTTGGRYHLNDH
                     HLDFNAKLFEESDEETIAGIIKHELCHYHLHLAGRGYQHKDMDFKYLLKKTGGLRYAP
                     ALTKKQIKMEYYRCQDCSATICRKRRIDVRKYRCGRCHGEIKWIETKREDFNEAQKIR
                     "
     gene            12291..13052
                     /locus_tag="U2K71_002276"
     CDS             12291..13052
                     /locus_tag="U2K71_002276"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HEM7316308.1"
                     /translation="MEKSEQRNLKDWNQLLNFLAAPEEGNFEELTAPTLILAGNCLPI
                     LADKAAQMYLNGQVDQLFLVGGVGHATRILYENFEKQGFHFEDGMSESEICRQYLKEV
                     YDLPDKAFLIESKSTNSGENATFSLEILHSLGAVPEKILLMNDPTLQRRTRATFEKVW
                     QNEQTIFANAVPFVPEILHFSEEIIFTAKELNHQWPKEYFHALVLGEMERLHDDENGY
                     GPKGKDFIPHIDISEEVWSSYTRIKESIKTDFSRT"
     gene            13183..14517
                     /locus_tag="U2K71_002277"
     CDS             13183..14517
                     /locus_tag="U2K71_002277"
                     /EC_number="6.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016624780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramate--L-alanine ligase"
                     /protein_id="HEM7316309.1"
                     /translation="MENQNKLYHFVGIKGSGMSSLALVLHEQGLNVQGSDIEKYFFTQ
                     RDLEKANITILPFNADNVKPGMTIIAGNAFPDSHEEIQRAKELGLEVIRYHDFIGHFI
                     QNYTSIAVTGSHGKTSTTGLLSHVLSGVRPTSYLIGDGTGHGDPQAEFFSFEACEYRR
                     HFLAYSPDYAIMTNIDFDHPDYYTSIDDVFTAFQTMAGQVKKAIFAYGDDAYLRKLKA
                     NVPIYYYGVTENDDIQARNIERTTSGSAFDVYHGDEFVGHFTVPAFGKHNILNALGVI
                     AVAYFEKLDLKEVAEEMLTFPGVKRRFSEKIVADMTVVDDYAHHPAEIKATIDGARQK
                     YPDKEIIAVFQPHTFTRTIALMDEFAEALDLADKVYLCDIFGSAREEQGNVKIEDLGA
                     KIKKGGEVIKENNVSPLLDYHDAVVIFMGAGDVQKFEQAYEKLLSSTTKNVL"
BASE COUNT         4772 a         2450 c         3215 g         4129 t
ORIGIN      
        1 gtttgttttt gcaccgttag aaggagaaga cctcgcttca tttctggttg gtgcggcaat
       61 catgatgatc ggaatgacgc tctttttgtt tggagcagat tattctatga tggaagtcgg
      121 agatctagta gggaaatata tgatcaaaaa gaaaagtttg accgttttgg tcagtttggg
      181 atttgctatc gggatcgtga tcacgattgc tgaaccttcc gtgcaagttt tgggacaaca
      241 agtatatgat atatctgacg gagagattgg cagagtgttg ctgattggga tcgtcagcgt
      301 cggcactgga gtatttctag cctttgcgct gcttcgggtc gtattcaagc tatcctatta
      361 tcaattgatg gctataggct acataggcgt tttgatcgcc tcatttttca ccagttccga
      421 atttatgccg attgcttttg attcgggggg agtgactact ggtccagtaa ctgttccatt
      481 tatcttggcc cttgccggag ggatgactag tatgatcaaa caaaagaaaa atgaaaatga
      541 cagttttgga atggtcggta ttgcttcgtt agggccgatt cttgccgtga tgattttggg
      601 ggtgatcttc caatgagttt ctggcagcaa gtgatagatg gaatcgaaat cgttaccatg
      661 gaagtcattt tggcagtggc acctatcgta atcctatttg ttttaatgaa tatcattagt
      721 tttcgtatgc gcaaaaagcg tttctttgac attatgaaag gtttcttgat aacgactatt
      781 gggttgatcc ttttcctgca cggagtcaat atggcttatg taccggtagg acagcattta
      841 ggcagtgcta ttgcgggatt gccttacaat tatatactgg ttccccttgg ctttttaatg
      901 gggttcttag tagggtttgc tgagccggcg attcatgtaa tgatcaaaca ggttgaagaa
      961 ttgagcgagg ggagaatccg atcgaaaatc atgctgatgg taatttcaat tgggatcggc
     1021 gcagcagtag gattgtctat gtggcgtttg ctcgtcggat tctctcttta ttatttcttg
     1081 atcccaggat acgggctagc atttatttta ggacgaaaag tggacaagat ctttttagca
     1141 atggcttttg ataacggtgg agtagcaacg ggcccgatgt gttcgacatt tattttatca
     1201 atgagcgtgg caatcgctgc acaaattgaa ggaagagatc cgatcattga cgggtttggt
     1261 gtggttgcaa tgattgcatt gacaccaata ttatccactt tagtactagg atatatttat
     1321 aaacgaaaag atgaaaaaga cagaatcgaa aaagaaagat tgcaaaaatt gggggaaagc
     1381 aaatgaaaga tcttgtagca ttgaatcttc acatgattgt gacgatcgtt gacggaggaa
     1441 tgggcgaaga tgttatcgac ttttcaaaag aagcaggtgc agcaggcgga acgatcttac
     1501 atggaagagg ttctggtgta catgattcag ggaaattttt aggtttggac atttcgccag
     1561 aaaaagatat tgtgttgaca ttagtacccg atagtttgac gaataaagta atggatgcga
     1621 ttggtcaagg catcaacatc gatattccag gtaatggtat ttgttttagt attgatgtag
     1681 acaaagtcat tggtatcact aagattcatc aatatcgaga aattatcgga atgcaagaac
     1741 ttttagatga aaataacgat agagaaaagt ctgaatcata gacaagcgtt tgaagaggaa
     1801 ataaacacaa acaagaaggc ggatgagcgt tatttccgct tttttgtttg tatttttctc
     1861 gtgaatactt atgaaatagt aaatttttat gaaattctac tctaagcatc agactttctt
     1921 ttcaaaatat gctattttaa aaatgatgaa aaaaggggga gaaaaatgaa ttttttagtt
     1981 gttttaaaag atactctgat tgagcgattt acatttctat atgttcaatt gcaggaaaat
     2041 aaaacattgc tgcatttttc accagagttc catctagagg ggctaacatt aggagaaatc
     2101 tatcaagcag aagggctgtc ggctgttttg cactttttta aggtagaatt ggaattacca
     2161 gtaaaagatt atatcgagct ttctcttgaa tcttgggatc gagcagtggt tgaactgttt
     2221 ccagaaggtt taatgatcga tacaaacgat gggaagatcc atctgactgc agcagaactg
     2281 caaaaacaaa tgcgttatca aatagatgac gaaaattctt tttctatttt tagacgtcag
     2341 caaaagattc taaaaagctt tttaaaagtg atcagtaaaa aacggaatct gatcaaaacg
     2401 acacagctgt tgaaaaaata tccaggtctg cttcacacgt ctattcaatt tcctcaattg
     2461 attacgatga tccatcgttt tatacgtatg caacagctac catctaagaa gctgtttctt
     2521 ccattgacgg acacatttcg agtagtcaac agagaagata aaaagaaagt gattgtgatt
     2581 gactttacca gaaatcggaa tgtccttcac caagttgcaa tggaaaaggc caattaaggc
     2641 tttttctttt tggatttttt tgtcataata aatattagat gatggaggag ataatatgat
     2701 gcaaacatgg ttagaaaggc taatacgatt tcagcagaac tatcacaaac ttatgcaggg
     2761 aagatatgcg aaatttgatc agttgaatcg cggtctattg atcgctgcgc ttgtctttag
     2821 tttgctaagt cgttggttgc cctatagaat cggacaattg ttgttcatcg tctttttcgg
     2881 atggatgata tttcgtttcc tatcaaaaaa aatctacccc agactaaatg agaatcaacg
     2941 ttatttgaaa tatcttgata gagtcaaaga aaaatatcat cagataaaaa acaaaaagca
     3001 agtcaaaatg gatcgaaaaa gttatacttt ttttgagtgc ccgaattgtc atcaaaagca
     3061 gcgtgcacct aaaggaaaag gacgaatacg tgtgacatgc aaaacatgtg gtataaaatt
     3121 cgaaacgaat gtttaaggga gaaaagaatg aaattagtat tattacgtca tggcgaaagc
     3181 gaagcaaatt ttgaaaatta ctggacagga tggctagatg tcgccttaac agaaaaagga
     3241 aaagaacaag cagcagttgc cggtcaaaaa atgcttgaag ccggacttca attcgatgcc
     3301 gtttatacct ctgttttgaa acgtgcgatt cttaccggac aagtcgcact agaagaaatg
     3361 ggtcagttat ggcttcctat aataaaaaca tggcgtctaa atgaacggca ttatggggat
     3421 cttgtgggta aaaacaaaga taagatgaaa gaaatatacg gaaaagatca agtaaaaaca
     3481 tggcgcagag gatatacgga agttccgccg ttaaccgcag aaaatagatt tgaccgcaga
     3541 tatgatcact tggatccgca tttgatccca tacggagaaa gtctggaaat gacagtgaat
     3601 cggatcattc ccttatggca agatcatcta gcaccaaaac taaaagatcg gcaagattta
     3661 ctagttgtgg gtcatggtaa tagcatccga gcactgacaa aatatttaga agatgttcct
     3721 gaagatcaaa tggatacgat cgatatccca aatgcacaac caatccagta tacattagca
     3781 gatgatttga caattctgga caaaaaaata ctttaaagaa cacctttatc taaaagtgag
     3841 agatagaaaa aagaagcaaa ggaatggaac aaaaggaaca tgaaaaatta tatatgggac
     3901 tttgatggta cattgtttga tacgtatccc gcaatggtag atggtgcatg gcaagcacta
     3961 aaagattttg gtatcagtat ggacaaaaaa gaaatctact tcaaaatgaa aaagtattct
     4021 actagttatt tgatcaatga atcaaacttg aatgccagag agtttaatga attatttcac
     4081 cgatatgaaa aagaaagtac ggaagtttcc cgtccatttc cagaaacgaa acaagtactg
     4141 gaaatgctaa aagacaacgg cggtcgtcat ttcatcctga cccaccgttt gacagaatca
     4201 acatggggcc tgttaaaaga acatcgattg gcacatctga ttgaggaagt agtagggatc
     4261 gatcaggact ttcctcgtaa acctgatcca gcatctttaa attatttgat cgatactttt
     4321 catttagaaa gaacagacac gatgatgatc ggcgatcgtc gtttagatat cgaagcaggg
     4381 aaaaatgcag gagtcgctac ttgtctttac gacatcgatc attttctggg tgagatcccg
     4441 gcagattatg tggtaggaaa tctgaatgaa atccttacct tgcagagcat cattaaaaag
     4501 tagtatcccc tttcaaagga gatatgttat aattttcata aaggagtgat aaaattatat
     4561 ggaaatcaga agacaaaaag aagttgtaga agcatttcat tatgatatga gattgccgaa
     4621 ccaagaagta gaaacagatt tgaaagtagg cttctcgcct ttaaaagaaa cgatcgaaaa
     4681 ctatccgaaa gaacatagca tcgtaggagc acgcttagag ttccgtttag tgtttgatac
     4741 atatgtttta tctggaagtg tgagtcagat caatcatatc ctcaatcgga aaattgaagg
     4801 tcaaaatgat gtcacacaag aagaagtgga cgagctagta gcaccgttat tcgatatcgt
     4861 acgccgaatg tcttatgagg tgacagagat cgccttagat cagcctgggg taaaattgaa
     4921 ttttcaatca gaaaaacaag aatagacagt aaaaacatac ctgcgataga ttaaagctat
     4981 cacaggtatg tttttttatt actaattgta agcaaaaaga atgtcatttc gataaaaatc
     5041 gtctaaaata taaaagtcac tgaaaaaaat tttttcagga ggatggttga agaatcattc
     5101 tcgggaacct ttacttcctt cgatggactt gtcattaata atatgaggag gagaaaaatg
     5161 acaacactca tcacagtttt agtggttcta gtttttgcgg cattattata tgtgttctat
     5221 cgtatgcaaa agcgccatac aaaattttca acacgcgtct ttacagcatt aggagttggt
     5281 atcgtctttg gcggtctgct tcaattgatc tttggtgtag acgacaaagt cgttactcaa
     5341 tcaatggaat ggatcgggat tgtaggtcaa ggatatgtat cattcttgca aatgctagtt
     5401 atgcctttag tgtttatttc gatcattggt gcattcacaa aaatgaaagt ttctgaaaag
     5461 ctagggaaaa ttagtgcgac tgtattgact tctttacttg gaacaacagc tattgcagca
     5521 ttgatcggaa ttggttcaac aatgctgttc ggactccaag gagctaaatt cacacaagga
     5581 gctgcagaaa cagcacgaat ctctgaactg gcaacacgtc aggaaacagt tgaaaactta
     5641 tcgattccac agcagatttt atcctttatc cctaaaaacg tttttgctga tttagcaggt
     5701 agtcgtgcaa caagtacgat tggagtcgtt attttcgcgg catttgtcgg aatcgcttat
     5761 ctaggcgtga aaagaaaagc gccaaaagaa gcagcattct tttcagattt agtcgacagt
     5821 ctgtataaaa tcattatgcg gatcgtgacg cttgttttgc gtttgacacc ttatggagta
     5881 tttgctttga tgacaaacgt tttggcaaca agtgattttg cagctatcgt caatttaggt
     5941 aaatttgtcg ttgcttctta cgtagcattg cttgctgttt tgattgtcca ctcactgatt
     6001 ttgattggtg caaaagtcaa tccaatcaac tatttcaaaa aagcctttcc ggtattgagc
     6061 tttgcgttta cttcacgttc aagtgctgga gctttgccat tgaatatcga gactcagaca
     6121 aaagctttag gagtagatga tgcaacagct aatttctcag caagttttgg tttgtctatt
     6181 ggacaaaacg gctgtgctgg cgtttatcca gctatgttgg caacgatcgt cgcaccagca
     6241 gctggagtga atgtctttag tttggaattt attttgatgc ttgtggcaat tgtaacaatc
     6301 agttcttttg gtgtagctgg agtcggtggg ggcgcaacat ttgcttcact gatcgtttta
     6361 ggggcaatga acttgccagt agcaatcgtt gggttagtta tctctgttga accattaatc
     6421 gatatggcac gtacagcagt aaacgtcagc ggtagcatgg tagctggtat cgtgacaagt
     6481 gcacgcatca aagatttaga tcgcgatgtc atcaatgatg attctaaagt gatcgatgtg
     6541 aatgcatagt caaaaagagg ttgtgataga agtggtcagc tttaagaaat aagaagaagt
     6601 ttcctaaaaa tagtttctca aatttttgtg aaatttcggc ttatttccga gaagctactt
     6661 ctgaaacacc gtgaataaga ggttgtgaaa aagctgccct gcatcaagta ataagaacag
     6721 ccaaacaaaa atagttcctc atattttacg tttggttgag cttattaccg cagatgcaag
     6781 cttttgaaca ccgtttaaga aagaggtcgt gagcctgccg tttcactctg agtcacaaag
     6841 agcaatttca aaaaatagct tctcatgttt gttgaatatt gtgtcttgtg accgaggagt
     6901 tgtcaggaga acaccgttta ccagaggttg tgacttgttt ttagtcacga cctctttttt
     6961 tatttattcc atataaatac acattattta ttccgaaaag tgaaagcgca aataacaaaa
     7021 acaaccaact tatttattat taaagaaaag cgtatttaag taggacgatt ctgttgagag
     7081 ctttcgtaga ttttcccatt ttcctatggt aaactgacaa agttgagaat gttagaacag
     7141 atcgtttagg aggaaacaga tgtttacgaa tgaaataaaa atcatgcttt atgtggacga
     7201 tgtagaaaaa aatgcaacgt tttggagagc agttggcttt gcggaaaaag atcgtcaaga
     7261 aatggatggg acgcttataa ttgaagtagg agttgaggac agtcaaacct catttctgtt
     7321 atatgatcga gcatttgttg agtcccattc acctggcaca gctactagtg ccccatcttt
     7381 gatgttttac agtaaagata tctttggact atataaaaaa atgcagacgc aatcagtcac
     7441 attaggtgat atggtacagc ttggagaaga atatgtcttt aactttgctg atcccgatgg
     7501 caactacttt gcggttacag gtaaataata gaaataaaga ctttgttttt ctgtgcgaaa
     7561 caggaaagcg aagtcttttt aattcaaata ataatattat gtaaacaaaa aactttccat
     7621 gcgtaattat acacaagcaa ataaatcgtc aaatgcgaac gccttcttta tactgtttag
     7681 gaacgaaaag aaggaaggaa gaaatacatg gattatcaag tactgcttta ttacaattat
     7741 acaaaaatcg aagaaccaga ggaatttgcc aaaaatcatt tagccttttg caaatctttg
     7801 gatcttaaag gtcgtattct agtagctgaa gaaggaataa atggtactgt gtctggaacc
     7861 gttgaaaaca cgactgccta tatgaaagca atgaaagctg atcctcgatt tgctgatacg
     7921 atgttcaaga tcgatccaag cgaaggacat gcctttaaga agatgtttgt aagataccgt
     7981 ccagagcttg taagtttaaa gctagaagag gacattgatc caaaccaagt gacaggtgcc
     8041 tatctttcac cgcaagaatt taaagaagct attttagatg aacagacagt ggtgattgat
     8101 gcacggaatg attatgaata tgacttggga cattttcgag gggctgtacg cccagatata
     8161 cgcagttttc gagagcttcc agactggatc cgcgagcaca aagaagaatt tatggaaaaa
     8221 cgggtagtga cctactgtac tggtggcatt cgttgcgaga agttttctgg ttggcttgtc
     8281 cgtgaaggat tcaaagatgt cggacagctg catggcggta ttgctactta tggtaaggat
     8341 cctgaagtcc aaggagaatt atgggacggc aagatgtatg tgtttgatga acggatcgct
     8401 gtcgatatca accaagtaga taaacaaatc gtcggtcgcg actggtttga cggaacacca
     8461 tgtgaacgat atatcaattg tagcaatcct gagtgcaatc gtcagattct tgcttccatt
     8521 gaaaatgaag aaaaacattt aggagcatgt agcatagagt gtgcgaaaca tccgcgcaat
     8581 cgctatgttc aagaccatgg aatctctcca gaagaagcag aactgcggtt tacagcttta
     8641 attaaataga aaacaatagt tttgattgtc gaccatttcc aatatgcgct atactagcgt
     8701 tgaggatggt gatccacatg aaatcattga tttatgctat tggggatgtc catggcaaac
     8761 atgagctttt tgaaaaactt attcactatt atgatccaaa gatccatcag cttgttttat
     8821 taggggatct caatgatcga ggtcctcgta caaaagaatg ttttttatta ggaaagcaat
     8881 tagtcgaaga aacaggagcc atttacctcc ggggaaacca tgaagaatat tttttgcaat
     8941 ttctgcaatc accggaggac tggtatgcgc cttatatgag aaatggtgga aaagaaacgt
     9001 ttgaaagcct gattcaccca ggggcatcgg aggaatactc cccaactgaa atctcgatga
     9061 tgatccaatc gagatattca gaactgatta aatttttagt ccagcgcccc ttgtattatg
     9121 aatgggaaaa ttatcttttt gtccatgccg gtgtggattt gacaaagaaa gactggcgta
     9181 agacaagtct aagagacttt atttggataa gggaaccatt tcatgaaggg aaaaacaata
     9241 caggtaagac aattgtattt ggacatacga ttacaccaat gctccatgga gatatgcaga
     9301 caacagacct ctggattcag gatcataaaa tcggaatgga cggtggggct gtattcggag
     9361 ggtcattgca tggggttgtt tttgacaagg aaggtattct tcaagatatt gaatatcaga
     9421 acaacgaagg accttggcat ggaatctgac taatggaaag aatgaaggag ctgtgacttt
     9481 gttgcggctc tttttatttt tatagagaat attgtttctt tcagtagttt aacccaggat
     9541 aaaaagtatg atagaataaa gaagcaattt ttagcagaaa agaggcattt taatgacgga
     9601 aaatttgaac cagacgatca ttcagcttgt acaaaaagaa ctttcagact atcgtcctaa
     9661 gcagttgact actgtactta atttgttaaa tgaaggaaac actgttcctt ttatcgcgcg
     9721 ttaccgaaaa gaaatgactg gaagtcttga tgaagtccag attcgtgaaa tcgaagagcg
     9781 gtatgcctat cttgaaaact tagaaaaacg taaaactgaa gtgattcggc taatcgatga
     9841 acaagggaaa ctgactcccg agttagaagc agaaatcacc caagccgtga aaatgcaaca
     9901 agtggaagat ttgtatcgtc catacaaaca aaaacgacgg acaaaagcaa cgattgctaa
     9961 agaaaaagga cttgaaccac tcgcgaagtg gctgatgcag cttacagatg gagaggtgca
    10021 atctgaagca gaaaaatata ttgacaaaga aaaagaagtc tcttctgccg aagaagcgtt
    10081 gcacggtgct catgagatta ttgctgaaca agtcagcgat aatgcaaaat ttcggacttg
    10141 gattcggtct tatacgtata acaaaggtat gtatgtcagc aatgtcaagg atgagcaagc
    10201 agatgaaaaa ggcgtatatg aaatgtatta tgactttgca gaacccgtcc ataaaatggt
    10261 ttctcatcga atattagcta ctaatcgtgg agaaaaagaa gatattctga aagtattctt
    10321 tcaagtagat gaagcagcaa ttttagctta cttggatcgc caaatagtca aaaatcctgc
    10381 ctctccttct tcttcattcg tcagagaagc ctatcaggat agttataaac gctttattca
    10441 gccagcaatc gagcgagaac tgcgcaatga attgactgaa aaggcagatg agcaagcaat
    10501 cgctattttc ggcgaaaacc tccgtaatct tttgttgcaa ccgccactaa aaggtaaagt
    10561 tgtcctggga tttgatccgg cttatcgtac tggatgtaaa ctagcagtcg tagatgcgac
    10621 agggaaagtt ttagcaatcg aagttattta tcctcataaa cctgcagcgc aagcaaaaag
    10681 agaagctgct ggaccagctt ttatccaatt aattaataaa tatcaagtag atatggtagc
    10741 tatcggaaat ggtacagcca gtcgggaatc tgaacttttt gtggctgagc agttgaaatc
    10801 cgccgaccat aaaacctatt acgctatcgt caacgaagca ggcgcttctg tttactccgc
    10861 tagcgaaatt gcacgaaaag aatttccgca tctacaagtt gaagaaagaa gtgctgtttc
    10921 tattgcccgt cgcctgcaag atcctttagc tgaattagtc aaaatcgatc ctaaagctgt
    10981 cggagtaggc cagtaccaac atgacgtttc tcaaaaacgt ttggctgaac aattggattt
    11041 tgttgtggaa acagcggtta accaagtagg tgtggatgtg aatacagcaa gcccgcagct
    11101 cttgcagcat atatcaggat tgaataaaac aactgcccaa aacattgtca tttatcgtga
    11161 agaaaacgga gaattcaccg cacgaactca attgaaaaaa gtgccacgct taggaccaaa
    11221 agcatatgaa caagcaatcg gattcttgcg agtccctggt gggaaaaaca ttttggacaa
    11281 cacaggtatc catcccgaaa gttattctat tgcaaaagaa atattaactt cagtccaact
    11341 ttcagaaaaa gagcttggta cagaagaagc agtagaaaaa ttgacacgat tatccgtaga
    11401 gaaactggct gaaagcctat cagtcggaga agaaacgtta gccgatattt tagctgggct
    11461 gacacaacct ggacgagata tgcgtgatga gatgccagcg cccttattac gtacagatgt
    11521 attgagtatg gaagacttga aaccagggat ggaattgact ggaacggtcc gtaatgtcat
    11581 tgacttcggc gcatttgttg atatcggtgt gaagcaagac ggacttgtcc atatatcgaa
    11641 actcagcaaa aaattcgtca agcatcctac agatgtcgtg tcagtaggag atatcgtaac
    11701 agtttggata gaacaagtcg atacgaaaaa aggcagaatc agtttgacga tgctttcccc
    11761 ttatgaagag taacaatgag gagtaacaaa aattgacaca agaagaactg cagcagttag
    11821 tagaagagat atcattaaaa agcttccagc ttcctttcaa gcaccaagca atgttcaatc
    11881 cacgcttacg tacaactggc gggcgctatc atttaaacga tcatcattta gattttaatg
    11941 cgaagctatt tgaagagtca gacgaagaga cgattgcagg aatcatcaaa catgaacttt
    12001 gccattatca tcttcatcta gctggaagag ggtatcagca taaagatatg gatttcaagt
    12061 atcttttgaa aaaaacagga gggttgcgct atgcaccagc cttgacgaag aaacagataa
    12121 agatggagta ttatcgatgc caagattgtt ctgccacgat ctgccgcaag agaagaatcg
    12181 atgtacgcaa atatcgttgt ggaagatgtc atggagaaat caaatggatt gagacaaaaa
    12241 gagaagactt taatgaagca caaaaaatca gatgaaagga tagaaggcct ttggaaaaaa
    12301 gtgagcagag gaatctaaaa gactggaatc agctattaaa ttttttagcc gcacctgaag
    12361 agggaaattt tgaggaactg acggcaccta cacttatttt agctggaaac tgtcttccta
    12421 tcttagctga taaagcagca caaatgtatt taaatggcca agtagatcaa ttgtttttgg
    12481 tcggaggggt ggggcatgcg actagaatcc tttatgagaa ttttgaaaag caaggatttc
    12541 attttgaaga tggcatgagt gaaagtgaaa tctgtcgtca gtatctaaaa gaagtctatg
    12601 atttgcccga taaggctttt ctgatagaat cgaagtctac aaattctgga gagaacgcca
    12661 ctttttcact ggagattctt cattccttag gagctgtacc agaaaaaatc ttattgatga
    12721 atgatccgac actgcaacgc aggactcgag caacttttga gaaagtatgg caaaatgaac
    12781 aaactatttt cgcgaatgct gtgccatttg tgccggaaat ccttcacttt tctgaagaga
    12841 tcatctttac agcaaaagaa ctgaatcatc aatggcctaa ggaatatttc catgcgctag
    12901 tattaggaga aatggaacgt ctccatgacg atgagaacgg ctatggtccg aaaggaaaag
    12961 attttattcc tcacattgat atatcagagg aagtttggtc ttcttacacc agaattaaag
    13021 agtcaataaa aacggatttt tctcgtactt gaccatttta gcaagcgaat cctcctttcc
    13081 aaataaattt ggttgaaatt ttcaatcgtt tataagataa tgaggagcgt gttgaatgta
    13141 atagctaaaa gaatggacgc tagataagag aagaggattc aaatggaaaa tcaaaataaa
    13201 ttgtatcatt ttgttggtat caaaggttca ggaatgagct ccttggctct tgttttacat
    13261 gaacaaggtc taaatgtcca aggatcagac attgaaaaat atttctttac acaaagagat
    13321 ttagaaaaag caaatatcac gattctgcca tttaacgcag ataatgtaaa accagggatg
    13381 acgatcattg caggaaatgc atttcctgat tcacatgaag aaatccagcg agcaaaagaa
    13441 ttaggtttgg aagtgattcg ctatcacgac tttattggtc attttatcca aaattatacg
    13501 agtatcgctg taacaggctc tcacggaaaa acaagtacga ctggactgct ctctcatgta
    13561 ttgagtggtg tccgtccaac aagttatcta attggagatg gaacaggaca tggtgatccg
    13621 caagcggaat tcttttcatt tgaggcctgt gaatatcgcc gtcatttcct tgcctattca
    13681 ccagactatg caatcatgac caatatcgat tttgatcatc cggattatta tacaagtatc
    13741 gacgatgtat ttacagcttt ccaaacaatg gctggacaag tgaaaaaagc tatttttgcg
    13801 tatggtgacg atgcttattt acgcaaatta aaagcaaatg tgccgattta ttattatggt
    13861 gtcacagaaa acgatgatat ccaagcacga aacatcgaac gcacaacttc tggttctgct
    13921 tttgatgtct atcatgggga tgaatttgtt gggcacttca ctgtaccagc attcggtaaa
    13981 cacaatatct taaatgcttt aggtgttatt gcagtagctt atttcgaaaa actggatctg
    14041 aaagaagttg cagaagaaat gctgactttc ccaggagtaa aacgtcgatt cagtgaaaag
    14101 atcgtagcag acatgaccgt agtcgatgat tatgcgcatc accctgcaga gatcaaagca
    14161 acgattgatg gcgcacgcca aaaatatcca gataaagaaa taattgcagt tttccaaccc
    14221 catacgttta cgcgtacgat tgcattgatg gatgaatttg cagaagcact tgatttagct
    14281 gataaagttt acttatgcga tatttttggt tcggctcggg aagaacaagg aaacgttaag
    14341 attgaagacc ttggagcgaa aatcaaaaaa ggtggagaag tcatcaaaga aaataatgta
    14401 tctccattgt tagattatca tgatgcagta gtaatcttca tgggtgctgg agatgttcaa
    14461 aaatttgaac aagcgtatga aaaactttta agcagcacga cgaaaaatgt actgtaatac
    14521 aattactgca gtgtccttat aaaaatagaa agagggggag tggcaa
//
LOCUS       DASHHB010000062        14077 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.064, whole genome shotgun sequence.
ACCESSION   DASHHB010000062 DASHHB010000000
VERSION     DASHHB010000062.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 14077)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14077)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14077
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.064"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..345)
                     /locus_tag="U2K71_002278"
     CDS             complement(<1..345)
                     /locus_tag="U2K71_002278"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEM7316310.1"
                     /translation="MIGEKMMDQKAFIESIDSTIDRLKKEIRFYNRVIGIGNIIKIVL
                     SASIPILIDQASEHRSLLLIVSIASAIITIIQSGMSAFNYQDKVQTSTELLMKIEKEK
                     LLYITKTSPYNKT"
     gene            complement(696..1388)
                     /locus_tag="U2K71_002279"
     CDS             complement(696..1388)
                     /locus_tag="U2K71_002279"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEM7316311.1"
                     /translation="MQIELQHTHDKETFEKNYKVYVELARDSAMKYGIPLTLDTPYNQ
                     PGIKSHLWVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDDNPTTSEDKPVIDP
                     TRAGAANSTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELAR
                     VRADGIYRPDVNQAYNTLGNVGYHVSFNMRDFPNKKVYVMMRATNDPEGNTKGGAQDF
                     HDKRWYLNIPQR"
     gene            complement(1825..2394)
                     /locus_tag="U2K71_002280"
     CDS             complement(1825..2394)
                     /locus_tag="U2K71_002280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HEM7316312.1"
                     /translation="MELKKLEVPTSSITEVKRSPMDVFAQARDAGTGVYIFNREKVAG
                     VMLTQEQYETLLQELDVLRQTVKVERKEPKEINEEPINKTASVVSNAEGMEELAQTLQ
                     HSLITGVTITAKNLDERMVALGFITKKTGFGGVVDMLSELMETGKINYQLRRKPQNRT
                     IIAEIIGEQNSQSQLMDKIIIKKIFLHEL"
     gene            complement(2468..2791)
                     /locus_tag="U2K71_002281"
     CDS             complement(2468..2791)
                     /locus_tag="U2K71_002281"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316313.1"
                     /translation="MLLLKTLQERGYLDVHPPEVKIVFFFRYEHQEARRLAGVLNKTL
                     RHRKRISIHVCTRNKPPRYADLVIIDHFFPLDHNEDQKTYLYHPSFGIERLLTDINYW
                     LGKNR"
     gene            complement(2945..3757)
                     /locus_tag="U2K71_002282"
     CDS             complement(2945..3757)
                     /locus_tag="U2K71_002282"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316314.1"
                     /translation="MRWQKLLSKNAQNLFSLINLMKDLIWRKADLAEELKIDVKTVDR
                     HLKRLAESDCPIQLILHKKTVQLVYDQGYSEPYLLFVFLIQSPPFCLLKNLLLNEPYE
                     QKYDRSTQERLRKWFGEYHLSFSYKKAQIFGSERKVRYLLFCFLKDFPHFFADDGEQS
                     FSEMFIQLLKQRKDPAEIEILGDSRTLFFELFPELLFREEQILPIKEKNYLAILRLSC
                     NQITEKRWKRIEETQDYRKLTIVTEKLDSLFWLNSEFESESKSKLTKVLYQE"
     gene            4111..4959
                     /locus_tag="U2K71_002283"
     CDS             4111..4959
                     /locus_tag="U2K71_002283"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008274428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HEM7316315.1"
                     /translation="MIVALLAGGHVLFEDIPGVGKTLLVKALAKAVQGTFSRVQCTPD
                     LLPSDILGFSVYNSQSKEFEFRPGPIFTTILLADEINRTTPRTQSALLEAMAENHATI
                     DNNTYPLDNHFFVLATQNPIEYEGTYPLPEAQLDRFLFRLQIGYPSFDDELLLLLDKF
                     EKVLDNLNEVLNSYEIEEIKTNVANVFVSPEVASYALQLVSATRSHAAIQLGISPRGS
                     LSFIQAAKAYALIHGRNYVTPKDLQDLVPYVFSHRLIFYDRSLKEDEKKQIIHTLVAQ
                     IPIPVR"
     gene            5104..6045
                     /locus_tag="U2K71_002284"
     CDS             5104..6045
                     /locus_tag="U2K71_002284"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304699.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HEM7316316.1"
                     /translation="MELDFIKQLSKETFGYLYIAATRLSQKKYAGYFFHSKKENFFFT
                     SPSFTNRLPSNLCRKKQEILILTNKPQEVQLLWQPKERGCHETLTVIFTAYDSLGLFR
                     KSAKREISFPVTVLPAFCKIQAEKLQRVLEKKQQLNAYQRGLDFREHRAYRPGDSFNR
                     IDWKLSAHTQEWLYREYDYQEEECSPLFCFWGSPSPKFEPLLDLYFSLWHLRKEKQPE
                     LLILGNRAFHGKDPGHTYFASLEAGDEKAFFAHLKKYAKKQIVLFVPENSPEVSEAIE
                     TLSKDRTVYLVYFVEKQLMVQEKDKICSLPGGEWIDD"
     gene            6035..8206
                     /locus_tag="U2K71_002285"
                     /pseudo
     CDS             6035..8206
                     /locus_tag="U2K71_002285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343611.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
     gene            complement(8318..10489)
                     /gene="copB"
                     /locus_tag="U2K71_002286"
     CDS             complement(8318..10489)
                     /gene="copB"
                     /locus_tag="U2K71_002286"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper/silver-translocating P-type ATPase CopB"
                     /protein_id="HEM7316317.1"
                     /translation="MNGNDMEKKHEHKKEVVNKESTESHEKQMEMKHDHSQMDHSMHM
                     GHNHGDIANSKQMDHSMHMDHEHGGMDHSMHMGNFKQKFWLSLILAIPIILFSPMMGM
                     EFPFQVTFPGSDWLVLILATILFIYGGQPFLSGAKMELKQKSPAMMTLIAMGITVAYI
                     YSVYSFIANLLNPHTHVMDFFWELATLIVIMLLGHWIEMNAVSNASNALQKLAELLPE
                     SVKRLTKDGKEETVSLKEVNEGDRLIVRSGDKMPTDGVILKGETIVDESAVTGESRGI
                     KKQANDKVIGGSINGDGTIEIEVTGTGENGYLAKVMEMVRKAQGEKSKLESLSDKVAK
                     WLFYVALIVGILAFIAWLFLTDLPNALERMVTVFIIACPHALGLAIPLVVARSTSIAA
                     KNGLLLKNRNALEQANDLDVIMLDKTGTLTEGKFTVTGVEVLDDAFNKNEILQYLGAL
                     EANANHPLAVGIMNYLKEHEIKPYQAENLKNLSGVGLEATVKKQQVKIVNEKEVERLG
                     LNVEQALLKPYQEQGNTISFLILENHLAAIVALGDVVKTEAKEFIRTLKERKITPVML
                     TGDNKNAAQAVADYLGIEEYYGGLLPDDKEAVVQKYLDQGKKVIMVGDGINDAPSLAR
                     ASIGMAIGAGTDIAIDSADVVLTNSDPKDILHFLDLAKQTRKKMIQNLWWGAGYNILA
                     IPLAAGILAPIGIILSPAVGAVLMSLSTVVVALNALTLKIR"
     gene            complement(10509..12695)
                     /locus_tag="U2K71_002287"
     CDS             complement(10509..12695)
                     /locus_tag="U2K71_002287"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HEM7316318.1"
                     /translation="MATAETKMDTFVITGMTCANCSARIEKELNGQPGVVNATVNLAT
                     EKASVKYEGTTTEKLIQSVENIGYGAILYDEAHKQKIAEEKQAYLKKMLFDLILSTVL
                     TLPLMLSMIAMMLGSHAAIVHFFHFPIVQLVLSAPVQFYVGARFYKGAYHAIKTKAPN
                     MDVLVAIGTSAAFALSIYNGFFRGHPQDLYFESSSMIITLILLGKYLEHTAKTKTGNA
                     IKQLMSLQTKTAQVIRNGKEETLAIEEVVVGDQLVIRPGEQIPADGRIISGSSAIDES
                     MLTGENLPVEKNPDDTLFGGTINTNGLLHMEVTQVGKQTVLAQIIQMVEDAQGSKAPI
                     QKIADRISGIFVPIVLVIAFITLIATGLITGDWQLALIHSVSVLVIACPCALGLATPT
                     AIMVGTGVGARNGILIKGGEALEAAAHLDSIVLDKTGTITEGKPKVTDLVGSKEVLSI
                     FYTLEQASEHPLGKAIVEYGKLQEAATYDMIDFTAHPGAGISGTINGVRYFAGTRKRL
                     IELNLSFDEYQEHALELEQQGKTVMFLADEKQVIGLIAVADQIKLEVKQAIKQLQNKG
                     LDVFMLTGDNKLAAETIGKQVGIDPKHIFAEVLPEDKAAYVEKLQKDGKKVGMAGDGI
                     NDAPALALADVGMAMGSGTDIAMETADVTLMNSSLASIAQTIELSRLTLRKIKQNLFW
                     AFVYNTIGIPFAALGFLNPIIAGGAMAFSSVSVLLNSLCLNRHMKK"
     gene            complement(12695..12904)
                     /gene="copZ"
                     /locus_tag="U2K71_002288"
     CDS             complement(12695..12904)
                     /gene="copZ"
                     /locus_tag="U2K71_002288"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone CopZ"
                     /protein_id="HEM7316319.1"
                     /translation="MKQQFSIEGMSCNHCAARVEETVSALDGVQKVKVNLKKANGTVK
                     FDETKVQSEKICQAINGLGYKAEVI"
     gene            complement(12917..13357)
                     /locus_tag="U2K71_002289"
     CDS             complement(12917..13357)
                     /locus_tag="U2K71_002289"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopY/TcrY family copper transport repressor"
                     /protein_id="HEM7316320.1"
                     /translation="MEKVKTPVKISDSEWEIMRVIWTLGQTTAQEITQILADSMDWKP
                     ATIKTLLGRLVKKEVIWTEQEGKKFIYYPAVSEMETVRSATENLFSHICAKRIGETIA
                     DLVEEATLTQEDINEILKQLEQKQPVDTIECNCIPGQCECKQHQ"
     gene            complement(13431..13970)
                     /locus_tag="U2K71_002290"
     CDS             complement(13431..13970)
                     /locus_tag="U2K71_002290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM7316321.1"
                     /translation="MKIIVIGQSGSGKSTLARKIKEITDFPLLPLDLLWHTTDYSIQA
                     KKWFLQEQQLFMVSNPSWIVEGNYTSTLEERIKEADKIIWLKVPRYLAIYRVVWRSLK
                     RKINKKSRPDMPESFSEKLNREYLEFLSFIWHFEENNEPKIQQLIQVANARNKLIILK
                     TRREKQEFLTKLKIESKNN"
BASE COUNT         3994 a         2905 c         2467 g         4711 t
ORIGIN      
        1 tgttttatta tatggggatg ttttcgttat atataacaac ttttcttttt caattttcat
       61 taataactct gtcgaagtct gtactttatc ttgataatta aatgcagaca taccactttg
      121 tataattgta attattgctg aagcaattga aacaatcaat agtaaagacc tgtgttcgga
      181 agcctgatca attaatattg ggatactagc tgataagact atttttataa tatttccgat
      241 accaattact ctattataaa atcgaatttc tttttttaat ctatcgattg tagaatcaat
      301 agattcgata aacgcttttt gatccatcat tttctctcca atcattttat taaaatcatt
      361 aacaaaaata ttataatagg atttaagtaa gttctaacta atcacatata aaaataaaaa
      421 aacatttgta ttttatcaaa gttttattat acttgtcaat ttgtgtacat cctaaaatat
      481 aagctatttg tttacttctg tagaatatat actttgttgt tagataagtt gcacatgttt
      541 atagaatcta aataataaat ttttatgtac cgcccctcaa agtgaactga accccaaaag
      601 ttgaactatt taatggactg tttccgatat tctactggag atagtccatt aaatagttca
      661 acttttgggg ttcagttcaa agaggggggg attttttatc gttgtggaat atttaaatac
      721 caacgcttgt catgaaaatc ttgtgctcca cccttagtat ttccttctgg atcgtttgtc
      781 gcacgcatca tgacgtatac tttcttatta gggaaatcac gcatattgaa agatacatga
      841 tagccaacat ttcctaaagt attataagct tgatttacgt ctggtctata aatcccatca
      901 gctcttactc tagctagttc ttttccagta ttgtagtcca taatgaaaat atactcgtat
      961 ttataattag caatgtgcca tccagctaca tgcaagtttg cgttttcgat ttctccgaac
     1021 tgatcaatgt gggcgtaatt tgttccatct gtcagcgtag aatttgcagc acctgctcga
     1081 gttggatcaa ttactggttt atcctccgaa gtagttggat tatcatccgt aaaaccatga
     1141 gccaaatcat aggctagttt ttctttactt acacccattt cagaaagata accgtaagga
     1201 tctgtatgat cgccccaaat attttgtgtt acccataaat gcgatttgat tcctggttgg
     1261 ttataaggag tgtctaatgt taatggaata ccatatttca ttgctgaatc tcttgccaat
     1321 tcaacgtata ctttatagtt cttttcaaaa gtttctttat catgtgtatg ttgtaactcg
     1381 atttgcacag gacttttggc tcacgaggtt gtcctactct acatcttgct gatagatatg
     1441 ctgaaaggaa aacaccaaca ttatgttttg ctttttcagc tagaacgaga caagcaggaa
     1501 tacctttaga agatatcaaa ggttttttag gtcataaaga tgtttccact acacaagtct
     1561 atgcccatat ttctcccgaa gtgaaaaaaa gatcaatgaa tcaactagaa aactatatag
     1621 aagagcaaat aaaaaagcac tcaaattgag tgctttttta agctcactat cactaaaact
     1681 atcactaatt ttatttataa gcgaacatgt ttctatcact gattttttaa atctagcgaa
     1741 aaacccttat cttaaaggcg ttcgttcaat tctttggcaa ggtcttcaaa gcctggcttg
     1801 cctagaagtg cgaacatggt tttcttatag ctcatgtaga aaaattttct tgatgatgat
     1861 tttatccatc aattgagatt gactgttttg ctctcctatg atttcagcta tgattgtccg
     1921 attttgcggt tttcttctta actggtagtt gattttccca gtttccatca actcgctaag
     1981 catatccacg actccgccaa atccggtctt tttcgtaata aaacctagtg ctaccattcg
     2041 ttcatctaga ttttttgctg taatcgtcac accagtaatg agcgagtgct gtaatgtttg
     2101 ggccagctct tccatccctt cagcattgga tacgacggaa gctgttttgt taataggttc
     2161 ttcgtttatt tcttttggtt ctttcctctc cactttcact gtttgacgaa ggacatctag
     2221 ttcttgcaag agcgtttcgt attgttcttg ggtcagcatc acgccagcta ctttttcacg
     2281 attaaatata tatacgccag ttcccgcgtc tcgcgcttga gcaaatacat ccatcggcga
     2341 tcgtttcact tctgtgatcg aagatgtggg aacttctaat ttctttaatt ccaatggatt
     2401 tcctccttta ctaatcgctt tttattatag cacagactga cttgttttga tcttttttcc
     2461 aaaggtttta tcggtttttg cctaaccagt aattgatatc ggtaagaagt ctttcgatac
     2521 caaatgaagg atggtacaga tacgtttttt gatcttcgtt atggtctagt ggaaaaaaat
     2581 gatcgatgat cactagatct gcataccgag gcggcttatt tcgagtacat acatgtatag
     2641 agatccgttt tcgatgtctt agcgttttgt tcagtacccc agcaagtctt cttgcttctt
     2701 ggtgttcata gcgaaaaaaa aagacgattt tcacttcagg agggtggaca tctaaatacc
     2761 ctcgttcctg gagtgttttc agtaaaagca tcccccactc tactgcgggg tgtgcaagtg
     2821 cttgttggat cgctagccga aattgatata gtttatcttt ggaattggga aatttttcta
     2881 atgtttcctg ataataacga acttcttcta tcagccggtc atggatacca attacgtaac
     2941 gaagtcattc ttggtaaagt acttttgtca gctttgattt gctttcgctc tcaaattcgc
     3001 tattcagcca aaacagcgaa tcaagctttt cagtcacaat cgtcaatttc cgatagtctt
     3061 gtgtctcttc tatccgtttc caccgctttt cagtaatctg gttacagctc aagcgcaaaa
     3121 ttgctagata atttttttct tttattggca aaatctgctc ctctctaaat agcaattcag
     3181 gaaacaattc aaagaatagt gttcgactgt ctcctaaaat ctcgatttct gccggatcct
     3241 ttctttgctt caataattgg atgaacatct cagaaaagga ctgctctcca tcatctgcaa
     3301 aaaaatgcgg aaaatctttc aaaaagcaaa acagcagata tcttactttt ctttctgaac
     3361 caaagatctg tgctttttta taagaaaaag aaaggtggta ctcgccaaac cactttctca
     3421 atcgctcttg cgtagaacga tcgtacttct gctcgtaagg ttcattcaat agcagatttt
     3481 tcagtaaaca aaacgggggt gattggatca gaaaaacaaa caagagataa ggttcgctat
     3541 atccttgatc ataaactaac tgaacggttt tcttatgaag aatcagctga atgggacagt
     3601 cgctttccgc caaacgtttc agatgccgat ccactgtttt aacatcgatt ttcagttctt
     3661 cagctaaatc agcttttcgc cagatcaaat ctttcattag attgattagt gaaaataaat
     3721 tctgtgcatt ttttgataat agtttttgcc atctcatcga tcatttctca cctgatttct
     3781 aaacatattg tttgatcaaa atgggttcac tcctaaaaag ataaaaaact ccctttctat
     3841 ttatttaaat acgcaaatct tcctcattat tttttatttt actcaattta atcgtattct
     3901 catttcaaaa cgaatgtact ttaaaaaaac ataataagat cactttgctt gtaggatgta
     3961 ttcccttcca ttacaatgaa ataaatagcg attttatgag aaggtgatgt gaaaatggaa
     4021 gaaaaaatag aagaatccaa acaaaaaatc gatcagttga tataagaatt agaaaaagcg
     4081 gtcatcggca aacgttcaac tatccaattg atgatcgtgg ccctattagc aggtggtcat
     4141 gtcttattcg aagatattcc cggtgtaggt aaaacgttac tggtcaaagc actagcaaaa
     4201 gctgtacagg gaaccttcag cagagtccaa tgtactcctg atctgctgcc tagtgatatt
     4261 cttggttttt ccgtatacaa ttcacaatca aaagagtttg aatttcgtcc gggaccgatc
     4321 tttaccacga tcttgttagc agatgagatc aatcgtacga ctcctcgtac tcagtcagca
     4381 cttttagaag caatggccga gaatcatgca acgatcgaca acaatacata ccctctagac
     4441 aatcattttt ttgtcttagc tacgcaaaat ccaatcgagt acgaaggaac atatcctttg
     4501 ccggaagcac agctcgatcg ttttttattc cgactacaaa tcggttatcc ttcttttgat
     4561 gacgaacttt tgctactttt ggataaattc gaaaaagtgt tggataactt aaatgaagta
     4621 ttgaacagtt atgaaataga agaaataaaa acaaatgtgg cgaatgtctt tgtcagtccc
     4681 gaagtggcat cttatgcctt gcagttagtg tcagcaacaa gaagtcatgc tgccatccag
     4741 cttggcatca gtccacgagg aagcctttcc tttattcagg cagcaaaagc ttatgcttta
     4801 atccatggga gaaattacgt cacgcccaaa gatttgcagg atttagttcc ttacgtgttt
     4861 tcccatcgcc tgatttttta cgatcgttca ttgaaggaag acgaaaagaa acaaatcatt
     4921 catactcttg ttgcacaaat tcctattcct gtgaggtaga ttgaggtaga caatggaaaa
     4981 aatcaaagat tatttcatta attttgtgtt atttctggta tatttcatca ttgtgctttt
     5041 tgctcttgta ttcaacaatt ctttaggctg gttcttgttg gcctttttta ctctttatgt
     5101 atcatggagc ttgatttcat taagcagctt tctaaagaaa ctttcggtta cttgtacatt
     5161 gcagccactc gtttatcgca aaaaaagtat gcaggttatt ttttccatag caaaaaagag
     5221 aactttttct ttacctctcc ctcgtttaca aatcggcttc ccagcaatct ttgcagaaaa
     5281 aaacaagaga ttttgattct tacaaataaa ccgcaagaag tccaattgct ttggcagcca
     5341 aaagaaagag gatgccatga aacattaact gtcattttta cagcctatga tagtctagga
     5401 cttttcagaa aaagtgcaaa aagagagatc tcttttcctg tgactgttct gcctgctttt
     5461 tgtaagattc aagcagaaaa attacagaga gttctggaaa aaaaacagca gctgaacgcg
     5521 taccaaagag gtcttgattt tcgggaacat cgcgcttatc gtccaggtga ttctttcaac
     5581 cgaatcgatt ggaaattgtc tgctcatact caagagtggt tataccgtga atacgattat
     5641 caagaagaag aatgttctcc tcttttttgc ttttggggtt cgcccagtcc aaaatttgaa
     5701 cctctgctag acttgtattt ttctttatgg catttgagaa aagaaaagca gccggaactt
     5761 ctgatattag gcaaccgcgc ctttcatggc aaagaccccg gtcataccta ttttgcttct
     5821 ttggaagctg gcgatgagaa agcttttttc gcacacttga aaaaatatgc aaaaaaacaa
     5881 atcgtcctct ttgttcctga gaattctcct gaagtctcag aagctattga aacattgtca
     5941 aaagatcgaa cggtttatct tgtttatttc gtagaaaaac aactcatggt ccaagaaaaa
     6001 gacaagatat gttcattgcc aggaggtgaa tggattgacg attaaacggg aaaaatggct
     6061 gcgtggcgtt tactcctttg tgatactaag tgtaggtgct ccgatatttg tagtagctaa
     6121 tcaattgcaa aacagctacc ttcttatcct aactattggt gtcctatgcc ttattgtctg
     6181 ctgtattcct aaaaaatggg tacgtttgct gctttctttg ccagtaatga taggttgttt
     6241 gtttttatat tttccttcga agacattttc catattatgg atcttacagt ttttttcgtc
     6301 agtcacagag gaattcttcc atctatccca aggtaaactt tattatttgc cagaaaagac
     6361 agcttttttt ctcataatgt tgctgatcta tttatttatt acattgaccg tggattacga
     6421 ctattggtac gcaccctttc tttctttgat ggcttacttc atgatgctta ccgtttttcg
     6481 gaaaaaagat ttactgtacg aaatgattgc ggtagtaact gttttgttcg tgtatctggc
     6541 agcaaggcaa tttttctcca tccggttgtt gtcgggaaac actcgattcc agtcattgac
     6601 tacagttctg cttatttgtt tcttaacttt tagcgctatc ttgcctttat atcttccgaa
     6661 agtacaccaa tctctcgagg aaacgagtga accgatccga gaatatctta atgatgaagg
     6721 gctttatgac acactccatg agtatcaatt aaccggaagt gcaagaacag gcttcagtga
     6781 gaatgatgaa cagcttggtg gacctttata tgataatggc gaagttgttt ttacagcgaa
     6841 ccaaaaagga aatggctatt ggaagatcga aaataaaaat cattacaccg gttctggatg
     6901 gatagaagat caggacgcac ccatccaacc aatccaagaa ttccctgttg acatcacgag
     6961 ctaccaaacc gttatagaag acaaagaaaa cattacattg accctaaaaa aacaagtttt
     7021 cttaccttat ccttatggaa atactacctt gactgatttt tcattagcta gtggacaaac
     7081 gctttactat ttgcctgaaa gccagcgatt cattcctgtc ccgaatgaat cgacacgttc
     7141 tgcaacttta caaacagaaa ggttagtttt tacgagagaa gaattaaaaa gcagtcagtt
     7201 aaaagctgat gatttagaaa atcagcagac aaatcttcaa ttgccaaaaa gtttgcctaa
     7261 acgtattgag gacttggcgg tcgaattgac caaaaatgaa gcaactttaa ttcgtaaagt
     7321 cgaagctgtc taacagtatt taaaaacaag cggaaatttc cgctattcca aaacggatac
     7381 cgggcatact ccaaaagaac acgattatgt ggattatttc ctctttgatt caaaagtcgg
     7441 ctattgtgac aacttttcaa cttctatggt cgtccttctt cgaacacttg gtatcccagc
     7501 tcgctggaca aaaggtttta caaatgggct cagaacaggc acagataatc aagggaatga
     7561 ggaatacagc attctcaata gtcatgctca ttcttggcca gaggtatact ttcctggatt
     7621 tggctggctt ccatttgaac caaccccttc atttgccaat ccaatgggtt cacaaccagt
     7681 tgctgaaaca accgaaaccg tccagcctgc tatcgaacaa ccaaatgaaa gcgaaaatat
     7741 ggcagaaact atttccagtg agagatctga ggtcattcca tcagaagaaa caacactcac
     7801 accagcttat aaaatcgtct tattcagcct tggagtgtgt ttatccgtct tgttgttctt
     7861 tctattgtta ttcagaaaat ctctgcgctg gacaagttat cttatctatc ttcatttttt
     7921 tgcttctact aatacgattg catatcaaaa acttcttaac cctttggaaa cattggtacc
     7981 gcgcaaaccc gcagaatctt tgcaggaata tagcctgcga atagaacaag aattcaagct
     8041 agatctgcgt ttcattcgat taactactca atatgaagca gaaatatatg gacgacaagc
     8101 agatccggta aaattcgata aaaaaaccta tttagaaata acgcatataa taagaaaaat
     8161 ggtatacaaa aaaattctga caaaagggag ccgcttcaaa aaataagata aaatttctgg
     8221 agaatctact tctggaatgc cgagaaccag ctgattgagt atcgattact cgaaaaaaag
     8281 gttgtgacac gttttttgtc acaacctttt ttcattatta tctgattttc aatgtcaaag
     8341 catttagagc tacaactacc gtacttaacg acatcaagac agcaccaact gctggactta
     8401 atatgatacc aattggcgca agaattccgg ctgctagcgg aattgcaaga atattgtaac
     8461 ctgcccccca ccagaggttc tggatcattt tctttcgcgt ttgcttggct agatcaagaa
     8521 aatgcaaaat atcttttgga tcgctgtttg tcaatacgac atccgcagaa tcgattgcga
     8581 tatctgtccc tgcaccgatt gccatcccga ttgaagcacg ggctaaactt ggcgcatcat
     8641 tgatcccatc acccaccatg attacttttt tcccttggtc aaggtatttc tggacaacag
     8701 cttctttatc atctggtaat aaaccgccat aatattcttc gatccctaaa taatctgcta
     8761 ctgcttgggc agcgttttta ttgtcaccag taagcatgac aggagtgatt tttctttctt
     8821 tcaatgttcg gataaattct tttgcttctg tttttactac gtcacctaaa gcaacgattg
     8881 cagctagatg attttctaaa atcaggaagc tgatggtatt tccttgttct tggtaaggtt
     8941 tcaataatgc ttgttctacg tttaatccga gacgctcaac ttctttttca ttaactattt
     9001 tcacttgttg ttttttgact gttgcctcta gcccgactcc tgaaagattt tttagatttt
     9061 cagcttgata aggcttgatt tcatgttctt tcaaataatt cataatgcca acagccaatg
     9121 gatgattggc attcgcttcc aaggctccta gatattgaag gatttcattt ttattgaacg
     9181 catcatcaag tacttcgaca cccgttacag taaattttcc ttctgttagt gttcctgttt
     9241 tgtccagcat gatcacatct agatcattgg cttgctccag tgcattgcgg tttttcaaaa
     9301 gtaatccgtt tttagctgct attgatgtgg aacgagcaac aactaatggg atagctaaac
     9361 ctagagcatg aggacaggcg atgataaaga ctgttaccat acgttctaat gcattaggaa
     9421 gatctgtcaa gaacaaccag gcgatgaatg ccaggatacc cacaattaat gcgacataaa
     9481 acagccattt tgccactttg tcagataatg actctagctt ggatttttct ccttgtgctt
     9541 ttcgcaccat ttccataacc ttcgctagat aaccattttc accagttcca gttacttcga
     9601 tttcgatcgt tccatcccca ttgatagaac cgccaatcac tttgtcattg gcttgttttt
     9661 tgattcctct tgattcgcca gtcacagctg attcgtcaac aattgtttca cctttcaaga
     9721 ttacaccatc tgttggcatt ttatcaccag accggacgat caaacgatca ccttcgttta
     9781 cttctttcaa tgaaactgtc tcttcttttc catctttcgt caagcgtttc actgattctg
     9841 gcagtaattc tgctagtttt tgtaaagcat tgctggcatt cgacacagca ttcatttcaa
     9901 tccagtgacc tagtagcata ataacgatca aagtagccaa ttcccagaag aaatccatga
     9961 cgtgtgtatg tggattgagc agatttgcga taaacgaata gacactataa atatatgcaa
    10021 ctgtaatccc catcgcgatc agtgtcatca tagcaggact tttttgtttc aattccatct
    10081 tagctccact taaaaatggc tgaccgccat agataaatag tatagttgct aagatcaata
    10141 cgagccaatc agaaccagga aacgttactt gaaatggaaa ttccattccc atcattggtg
    10201 aaaaaagaat gattgggata gctaggataa gcgatagcca aaatttttgt ttgaaattcc
    10261 ccatgtgcat ggaatgatcc atgccgccgt gttcgtgatc catgtgcatc gaatggtcca
    10321 tttgtttgct gtttgctata tctccatggt tatgtcccat gtgcatggaa tgatccatct
    10381 gactatggtc atgtttcatc tccatctgtt tttcatgaga ttctgtactt tctttgttta
    10441 ctacttcttt tttatgctca tgttttttct ccatatcgtt cccattcatt tttactccat
    10501 cccttctctt actttttcat gtgtcgattt aagcacaggg agttcaacaa tacactgacg
    10561 gaactaaacg ccatcgctcc tcctgcaatg attggattca aaaagcctag tgcggcaaaa
    10621 ggaataccaa tcgtattata gacaaaagcc cagaaaagat tttgtttgat ttttctcaat
    10681 gtcaacctag acaactcgat tgtttgtgca atggatgcca aactgctatt catcagcgtc
    10741 acatcggctg tttccatcgc aatgtccgtt ccacttccca ttgccatacc gacatcagct
    10801 aatgctaagg ccggtgcatc attgattcca tcgcctgcca taccgacttt tttgccatct
    10861 ttctgaagtt tttctacgta ggctgcttta tcctctggaa gtacttcagc aaagatatgc
    10921 tttggatcaa taccaacttg tttaccaatc gtttcggctg ccagtttgtt gtctcctgtc
    10981 agcataaaaa catctaatcc tttattctgg agctgcttga ttgcttgttt gacttctagt
    11041 ttgatttgat cagccacggc aatcaacccg atcacttgct tttcatcagc taaaaacatg
    11101 actgtttttc cctgctgttc taattctaac gcgtgttctt ggtattcatc aaatgatagg
    11161 ttcaattcaa ttaatcgttt acgtgtaccc gcaaaatagc gtactccgtt aatcgtaccg
    11221 ctgattccag cacctgggtg ggctgtaaaa tcaatcatat cgtatgttgc tgcttcttgc
    11281 agttttccgt attccacgat tgctttacct agtggatgtt cagaagcttg ttctaaagta
    11341 taaaaaatag ataggacttc tttagaaccg actagatctg taactttggg ctttccttct
    11401 gtaatcgttc cagttttatc caaaacaatg ctgtctaagt gagcagctgc ttctaatgct
    11461 tctccgcctt taattaaaat tccatttcgt gcaccaacac ccgttcctac cataatagct
    11521 gttggagtcg ctaaacctaa tgcacatgga cacgcaatga ctaatacaga gacactatgg
    11581 atcaatgcga gttgccaatc accagtgatc aaacctgtag ctatgagcgt gataaacgcg
    11641 atgactaata cgatcggaac aaaaatccct gagatccgat ctgcgatttt ttggattggg
    11701 gctttgctcc cttgagcatc ttccaccatt tgaatgattt gcgctaatac tgtctgcttg
    11761 ccgacttgtg taacttccat gtgaagtaga ccattcgtat tgattgtccc gccaaagagc
    11821 gtgtcatccg gattcttttc tacaggaaga ttttcaccag taagcatact ttcatctatc
    11881 gcactgcttc ctgaaataat tcgaccatct gctggaatct gctcaccagg acggatgact
    11941 agttgatcac caacgactac ttcttcaatc gctaacgttt cttctttccc gtttcttatc
    12001 acttgggcgg tttttgtttg aagagacatc aattgtttga ttgcattacc tgttttcgtt
    12061 tttgctgtat gttccaaata tttgccgagc aaaatcagcg tgatgatcat actgctgctt
    12121 tcaaaataaa gatcttgggg gtgtccacgg aaaaatccgt tgtaaatgct caatgcaaag
    12181 gcagccgatg ttccaattgc taccaataca tccatatttg gtgctttcgt cttgattgca
    12241 tggtacgcac ctttataaaa acgagcaccg acataaaatt ggacaggagc agacagcact
    12301 aactggacga ttggaaaatg aaaaaagtga acaatcgccg catggcttcc taacatcata
    12361 gcaatcattg acaacatcaa cggcaaagtc aaaaccgtac tcaatataag atcgaatagc
    12421 atttttttca aatacgcttg cttttcttct gcaatttttt gtttgtgggc ttcatcgtac
    12481 aaaattgcac catagccgat gttttctacg ctttggatta atttttcagt ggtcgtccct
    12541 tcatatttga cacttgcttt ttcagtggcc aagttgactg tggcattgac cacaccaggt
    12601 tgtccgttca gctctttttc gatcctagca gaacaatttg cacaagtcat acctgtaatg
    12661 acgaacgtat ccattttggt ttctgctgtt gccatcaaat cacctctgct ttataaccca
    12721 atccattgat tgcttgacaa atcttttcgg attgaacttt tgtttcatca aacttgaccg
    12781 taccgtttgc ttttttcaaa tttactttca ctttttgcac gccgtcaagt gcgcttactg
    12841 tttcttctac tcttgctgca caatgattgc agctcatacc ttcgattgaa aattgttgtt
    12901 tcattttttc ttcctcctat tgatgttgtt tacattcaca ttgacctggg atacagttac
    12961 attcgatcgt atctactggt tgcttttgtt ccagttgttt caaaatctca ttgatatctt
    13021 cttgagtcaa agtggcttct tctactagat ccgcaatcgt ctctccaatc ctttttgcac
    13081 agatatggga aaaaagattt tccgtcgcac tgcgaactgt ttccatctcc gagacagctg
    13141 gataataaat gaattttttt ccttcttgtt ctgtccagat cacttctttt tttaccaaac
    13201 gacccagcaa agttttgatc gttgctggtt tccaatccat cgaatcagct aagatctgcg
    13261 tgatttcttg ggctgtcgtc tgtccaagtg tccaaataac acgcatgatc tcccactctg
    13321 aatcactgat ctttactggc gttttgactt tttccattta tttcacacct ttcgtttaca
    13381 aatgtaatca ttatcttatg aatacagatt acacttgtaa acaaaaactg tcaattattt
    13441 ttgctctcta ttttcaattt agttaaaaat tcttgtttct ctcgtcttgt tttaaggatg
    13501 atcagtttat ttcttgcatt agctacttgg atcagctgct gtattttcgg ttcattgttt
    13561 tcttcaaaat gccagataaa agataaaaat tctaagtatt ccctgtttag tttctccgaa
    13621 aatgattcag gcatatctgg ccgagatttt ttgtttatct ttctttttag agaacgccaa
    13681 actactcggt agatggcgag ataacgcggc actttaagcc aaatgatctt atctgcttct
    13741 ttaatccttt cctctaatgt agaagtataa tttccttcaa cgatccatga cggattagat
    13801 accataaaca attgctgttc ttgcaaaaac cacttttttg cctgaatact gtagtctgtt
    13861 gtatgccata aaaggtctag cggcagtagc ggaaagtcgg tgatttcttt gatcttcctt
    13921 gcgagggtcg attttcccga cccactttgt ccaatcacga tgatcttcat tcttctccct
    13981 cacttctctt tggaatttgg ttcgatcata ccacttataa cgccgcaaaa aaacaaggga
    14041 ctgtgacaaa agtcgtgtca aagtcccttg tttcgaa
//
LOCUS       DASHHB010000063        13735 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.065, whole genome shotgun sequence.
ACCESSION   DASHHB010000063 DASHHB010000000
VERSION     DASHHB010000063.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13735)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13735)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13735
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.065"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            351..521
                     /locus_tag="U2K71_002291"
     CDS             351..521
                     /locus_tag="U2K71_002291"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000713595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316322.1"
                     /translation="MKKFIYRVLENDEVVAIFNEQQYAQDFIAYEKTISDKQFEIEKV
                     DIADWLLQPREF"
     gene            535..1137
                     /locus_tag="U2K71_002292"
     CDS             535..1137
                     /locus_tag="U2K71_002292"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7316323.1"
                     /translation="MAKIGYARVSSKEQNLDRQLKALEGVSKVFSDKLSGQSVERPQL
                     QAMLNYIREGDIVVVTELDRLGRNNKELTELMNQIQIKGATLEVLNLPSMNGIEDENL
                     RRLINNLVIELYKYQAESERKRIKERQAQGIEIAKKKGKFKGRQPKFKENDPRLQHAF
                     DLFLNGCSDKEVEEQTGINRRTFRRYRSRYNVTVDQRKKQ"
     gene            1153..1830
                     /locus_tag="U2K71_002293"
                     /pseudo
     CDS             1153..1830
                     /locus_tag="U2K71_002293"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000108743.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase"
     gene            2141..2305
                     /locus_tag="U2K71_002294"
                     /pseudo
     CDS             2141..2305
                     /locus_tag="U2K71_002294"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000898188.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
     gene            2342..2638
                     /locus_tag="U2K71_002295"
     CDS             2342..2638
                     /locus_tag="U2K71_002295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002360685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA recombinase"
                     /protein_id="HEM7316324.1"
                     /translation="MSKGFLEPALFNQERNVLDSEIKNLTTEKTNLVTNSASGVLRAN
                     EIKDLINYVSADNFNGDYTEELFEEFVVNIIVNSRDELTFNLKCGLSLKEKVVR"
     gene            2639..3055
                     /locus_tag="U2K71_002296"
     CDS             2639..3055
                     /locus_tag="U2K71_002296"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000323438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase"
                     /protein_id="HEM7316325.1"
                     /translation="MAYIPYGYKIQDGVVTVDEKAAGQVKVFFEKYISGLSLTVAGEQ
                     AGIEKTHSVMGRILKNVNYLGNDTYPAIIDKEIFDKAEEVRDKRAKDLGRVVELAAFT
                     SPPPKERFKMKKADNKMPVDPFERAEYLYSLIESEE"
     gene            3075..4619
                     /locus_tag="U2K71_002297"
     CDS             3075..4619
                     /locus_tag="U2K71_002297"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018659190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7316326.1"
                     /translation="MVIPARKRVGSTAAKEKIKKLRVAAYCRVSTETEEQNSSYEVQV
                     SHYTEFIKKNNEWEFAGIFADDGISGTNTKKRDEFNRMIAECMDGNIDMVITKSISRF
                     ARNTLDCLQYIRQLKDKNISVYFEKENINTTDAKGEVLLTIMASLAQQESQSLSQNVK
                     LGLQYRYQQGKVQVNHKRFMGYTKDEDGNLTIVPEEAEIIKRIYREYLEGQSLVGIGR
                     ALEKDGILTAAGKPRWRPESVKKILQNEKYIGDALLQKTVTVDFLTKKRVKNEGHVPQ
                     YYVENSHEAIIPKELFLQAQEEIHRRSNIYTGEGKNKRIYSSKYALSAITFCGDCGDI
                     YRRVYWNIHGRKEFVWRCVTRIEQGPEVCKNRTVKEDELYGAVMTAINKLLAGGNNMI
                     KTLEENIHAVIGETTEYQISEINNSLEEKQKELIKLANKGQDYDHLADEIDELRDKRQ
                     ILLVEDASLSGENERINELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEGHFVIAFKS
                     GIEMEI"
     gene            4762..4986
                     /locus_tag="U2K71_002298"
     CDS             4762..4986
                     /locus_tag="U2K71_002298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7316327.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGEEIKISNE"
     gene            5001..5870
                     /locus_tag="U2K71_002299"
     CDS             5001..5870
                     /locus_tag="U2K71_002299"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEM7316328.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            5851..6585
                     /locus_tag="U2K71_002300"
     CDS             5851..6585
                     /locus_tag="U2K71_002300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM7316329.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            6618..7526
                     /locus_tag="U2K71_002301"
     CDS             6618..7526
                     /locus_tag="U2K71_002301"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEM7316330.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            7523..8065
                     /gene="sat4"
                     /locus_tag="U2K71_002302"
     CDS             7523..8065
                     /gene="sat4"
                     /locus_tag="U2K71_002302"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEM7316331.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            8158..8952
                     /locus_tag="U2K71_002303"
                     /note="aph(3')-IIIa"
     CDS             8158..8952
                     /locus_tag="U2K71_002303"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEM7316332.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            9228..9800
                     /locus_tag="U2K71_002304"
     CDS             9228..9800
                     /locus_tag="U2K71_002304"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEM7316333.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
     gene            10098..10994
                     /locus_tag="U2K71_002305"
     CDS             10098..10994
                     /locus_tag="U2K71_002305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002355409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM7316334.1"
                     /translation="MIQYYYTKKEWGVVMEKEKLKILEELRRILNNKNEAIIILNNYF
                     KGGVGKSKLSTMFAYLTDKLNLKVLMIDKDLQATLTKDLAKTFEVELPRVNFYEGLKN
                     GNLASSIIHLTDNLDLIPGTFDLMLLPKLTRSWTFENESRLLATLLAPLKSDYDLIII
                     DTVPTPSVYTNNAIVASDYVMIPLQAEEESTNNIQNYISYLIDLQEQFNPGLDMIGFV
                     PYLVDTDSATIKSNLEELYKEHKEDNLVFRNIIKRSNKVSTWSKNGITEHKGYDKKVL
                     SMYENVFFEMIERIIQLENEKE"
     gene            11093..11302
                     /locus_tag="U2K71_002306"
     CDS             11093..11302
                     /locus_tag="U2K71_002306"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEM7316335.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
     gene            11319..11591
                     /locus_tag="U2K71_002307"
     CDS             11319..11591
                     /locus_tag="U2K71_002307"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEM7316336.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            11593..12456
                     /locus_tag="U2K71_002308"
     CDS             11593..12456
                     /locus_tag="U2K71_002308"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM7316337.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(12719..13456)
                     /gene="erm(B)"
                     /locus_tag="U2K71_002309"
     CDS             complement(12719..13456)
                     /gene="erm(B)"
                     /locus_tag="U2K71_002309"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEM7316338.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(13581..13664)
                     /locus_tag="U2K71_002310"
     CDS             complement(13581..13664)
                     /locus_tag="U2K71_002310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEM7316339.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
BASE COUNT         4868 a         2134 c         2852 g         3881 t
ORIGIN      
        1 aagaagcatt ttattctagc ttaatttgac aaatcatact atgatacgtg tttgtttttc
       61 tgtgctgttt agtgaatgat tagcagaaat atagagagta agattttaat taattattag
      121 ggggagaagg agagagtagc ccgaaaactt ttagttggct tggactgaac gaagtgaggg
      181 aaaggctact aaaacgtcga ggggcagtga gagcgaagcg aacacttgat cttttaagtt
      241 gctatctttt taaggtcaat agagtatact tatttgtcct aatatggttt tagcagtata
      301 attgacttgg tgaataggtc atttaagttg ggcataatag gaggagtaaa atgaaaaaat
      361 ttatttatcg agttttagaa aatgacgaag tggtggctat ttttaatgag caacaatatg
      421 cgcaagattt tatcgcttac gaaaagacaa tttctgataa gcaatttgaa attgaaaaag
      481 tagatatagc tgattggtta ttgcaaccga gagaatttta gaggttggtt gaaaatggct
      541 aaaattggtt atgcacgtgt cagtagcaaa gaacagaact tagatagaca actgaaagcg
      601 ttagagggcg tttctaaggt cttttcagac aaattaagcg gtcaatcggt cgaacgccca
      661 caattacaag ctatgcttaa ctatattcgt gaaggggata tagttgttgt tactgaatta
      721 gatcgattag gacgaaataa taaagaatta acagaattga tgaatcaaat tcaaattaag
      781 ggggcaaccc tggaagtctt aaatttaccc tcaatgaatg gtattgaaga tgaaaattta
      841 agacggctga ttaataattt agtgattgaa ttgtataagt accaagcgga atctgaacgc
      901 aaacgaatta aagaacgcca agcccaagga attgaaattg ctaagaaaaa aggaaaattc
      961 aaagggcgac aacctaaatt caaagaaaat gatccacgtt tacaacacgc tttcgattta
     1021 tttttgaatg gttgttccga taaagaagtt gaagaacaaa ctggaattaa tcgccgaacg
     1081 tttagaaggt atcgatcaag atacaacgtg acagtagatc aaagaaaaaa acaatgaaaa
     1141 gagggatagt taatgagtac ggttatttta gctgaaaaac caagccaggc attagcctac
     1201 gcaagtgctt taaaacaaag caccaaaaaa gacggttatt ttgagatcaa agacccacta
     1261 tttacagatg aaacgtttat cacctttggt tttgggcatt tagtcgaatt agcagaacca
     1321 ggtcattata acgaaaagtg gcagaactgg aaacttgaat ctttgccgat ttttcctgat
     1381 cgatacgatt ttgaagttgc aaaagataag ggaaagcagt ttaaaattgt tgctgaactt
     1441 ctcaaaaagg caaatacaat tattgtcgca acagatagcg acagagaagg tgaaaatatc
     1501 gcctggtcga ttatccataa agcaaatgcc ttttctaaag ataaaacgta taaaagatta
     1561 tggatcaata gcttagaaaa agatgtgatc cgaagcggtt ttcaaaattt gcaaccagga
     1621 atgaattact atccctttta tcaagaagcg caaacacgcc aaattgccga ttggttgatc
     1681 ggcatgaacg caagcccttt atataccttg aatttacaac aaaaaggcgt acaaggtaca
     1741 ttttcattag gacgtgtaca aacaccgact ttatatctca tttatcaacg ccaggaagca
     1801 atagaaaact ttaaaaaaga accttttttc ctgaataatt catagtttta ctaatagtct
     1861 ttcctaacat tgatttaata gtctttaaag tattctgctt catcacccat taggtgatga
     1921 aaaaagaacc cctttctgat ttagcggaca aatactttga cctaatctca gaagatataa
     1981 aagaaaaaat aataaaatat ccgatttata ctttgtataa agaactaaag tagttagcaa
     2041 atcatttaca gcaaatcact atgttccaca taccatttta catacttacc ttatccacga
     2101 cacatgttga gatagtatcg aagataaaga aagttgctcc atgttgtttt tgcgagatgg
     2161 ggaaatcaaa gccacattga caaccatgat gaataagctt gctttcagtc ataagctaat
     2221 cctagaacca cttttcaagt cagttagcca aatcgatgaa gaaagcgacc gtgaaagaat
     2281 ggacgctatt gataagctaa tggagcaact attggaagaa cgcaataccc ttattgcact
     2341 gatgtccaaa ggtttcctag aacctgctct ttttaatcag gaacgaaatg ttttagatag
     2401 tgagattaaa aatcttacta ccgaaaaaac aaacctggta acgaactctg cgagtggggt
     2461 tttgcgagca aacgagataa aggacctcat taattatgtg tcagcagata attttaatgg
     2521 tgactacacg gaagaactat ttgaagaatt tgtagtgaac atcattgtaa attccaggga
     2581 tgagctgaca ttcaatttga aatgcggtct ttccctgaaa gaaaaggtgg tgagataaat
     2641 ggcatatatt ccatatggat acaaaattca agatggagtg gttactgtcg atgaaaaggc
     2701 agcaggtcaa gtaaaggtat tctttgagaa atacatatca ggactatccc ttacagtggc
     2761 tggcgaacag gcaggtattg aaaagacaca ttctgtgatg gggcgcattt tgaaaaacgt
     2821 caactacctt ggaaatgata cgtatccagc aattattgat aaagagatat ttgataaagc
     2881 tgaagaagtt agagataaac gtgcaaagga tttaggacga gtggtagagc ttgccgcttt
     2941 cacctctccc cctcccaaag aacgattcaa aatgaaaaag gcagataata agatgccagt
     3001 ggatcctttt gaacgagcag aatacttata cagtctgata gaaagcgagg aataaagtga
     3061 cagagaaaaa tataatggtt attcctgctc gtaaaagagt aggaagtaca gccgcaaaag
     3121 aaaagataaa gaaacttcgt gttgctgcct attgccgtgt ttctacagaa acagaagaac
     3181 aaaattctag ctatgaggtt caggtctcac actatacaga gtttataaag aaaaataatg
     3241 aatgggagtt tgctggcatc tttgcagatg atggtatctc tggtacgaac actaaaaagc
     3301 gtgacgaatt taatcgcatg attgcagagt gtatggacgg taacatcgac atggttatta
     3361 caaaatccat cagccgattt gcgcgtaaca ccctagactg ccttcaatac attagacagc
     3421 tcaaggataa gaacatatca gtttattttg agaaagagaa catcaacacc acggatgcca
     3481 aaggtgaggt tttgctgact attatggcat ctttggcaca gcaggaaagc cagagccttt
     3541 cacaaaacgt taagcttggg ctacagtacc gataccaaca aggaaaggtg caggtcaacc
     3601 ataagcgatt tatgggctac acgaaagatg aagatggaaa tttaactatt gtccccgaag
     3661 aagctgagat tatcaaacgc atctaccgag aataccttga gggtcagagt ctagtgggca
     3721 ttggtcgagc ccttgaaaag gatggtattt taacagcagc gggaaaacca agatggcgac
     3781 cagaatcagt taagaagata cttcaaaacg aaaaatacat cggagatgcc cttctgcaaa
     3841 agactgtcac agtagatttt ctgaccaaga aacgagtgaa aaatgaaggg catgttcccc
     3901 aatattatgt tgaaaatagc catgaagcga ttattcctaa agagttattt ttgcaggcgc
     3961 aagaagaaat tcatcggaga agcaatatct acacaggaga aggcaagaac aaaagaattt
     4021 acagtagcaa gtacgcttta agtgccatca ccttctgtgg agattgtggc gatatttata
     4081 gacgggtcta ttggaatatc cacggaagaa aagaatttgt ctggcgatgc gtgactagaa
     4141 tcgagcaagg tcctgaagtt tgtaagaacc gaaccgtaaa agaagatgaa ctttatggtg
     4201 cagtaatgac tgcgattaat aagctacttg caggtgggaa caatatgata aagacactgg
     4261 aagagaatat tcatgctgtg attggcgaaa ccacagaata ccaaatttca gagattaaca
     4321 actcactaga ggaaaaacaa aaagaactaa tcaagctggc gaacaagggt caagactatg
     4381 accacttagc agatgagatt gatgagctga gagacaagcg acagatcctt ttagtagaag
     4441 atgcctccct cagtggcgag aacgagcgaa tcaatgagct gattgaattt atccgcaaga
     4501 acaaattccg caccttagaa tatgatgata agcttgtaag gaagataatc cagagcgtta
     4561 cagtctatga aggccacttc gtcatagcct tcaaatctgg catcgaaatg gaaatatgaa
     4621 aaccagaagt aaatagccca tgactctaca gtagagttgt gggttttctt ttgcttgctg
     4681 attataataa tattttaata actattgtgt ttatcaacca aatggtttat aatattctta
     4741 gcgtaaactt ggaggtgctg tatgactacg gcagaaatga ttaaagaact gtgtgagcaa
     4801 atgaatataa gtgtttccga acttgctaga cgtattggcc agactccaca gaatttcaat
     4861 aaaaaattac aacgagaaac agtaaccttg gatgagttga aggccattgc tgatgtgcta
     4921 ggtgtcaagt ttgtgcaggc atttatttta ccagatggag aagaaataaa aatatctaac
     4981 gaataaagga ggatgtttat atggtaggta atgttattca aatagtaaca gagaaattat
     5041 catctttgcc ttttatagaa ggcatcgtat tagggggctc acgtgcaaga ggcacccata
     5101 cagaaaattc tgatatagat atcggaatct attacaattc agactcattt gacctgacag
     5161 cgattaatca aattgctaca gagttggatg atgagaatag aaacaacctt gttgtacctc
     5221 ccggagcgtg gggtgattgg gttaatggcg gtggatggtt agttataaac gggtgtcatg
     5281 ttgacttgat attacgtgat ataaaacggg tggaacaaat aatcaaagat acggagcaag
     5341 gaattgttac tgccaattat cagaccgggc atccccatgg ttatataagt gctatgtatc
     5401 gtggagaatt agcgattagt aaaatactat atgctaagaa cgaaagctta tgcgaattaa
     5461 aaaagcaggc agaaatttac cctactgctc taaagaaaag cttgatgaac ttttttatat
     5521 ttgaagctga attctcttta atgtttgtaa aagcaaatgc gggggcagag gataaatatt
     5581 atattgcagg ccatgttttt cgtattattt catgcttaaa tcaagtacta tttgcatgta
     5641 ataatgctta ttgcattaac gaaaagaaag ctataaaact gcttgaaact tttgaatata
     5701 aacctgaaaa atatgccgaa agagttaatc atatttttga agtactcggt ctttctcttt
     5761 ttgaatgcta cgacatgacc gagaaacttt ataaagaagt gaatgaaatt gtatcggaga
     5821 taaataactt tttaaacgag gagagttcag atgaaagaaa acaaatatga tgataatata
     5881 ttttttcaaa aatacagtca aatgagtcgc tcgcagaaag gactggctgg tgcgggagaa
     5941 tgggagactt tgaaaaagat gctacctgat tttaagggta agcgtgtgct tgatttagga
     6001 tgcggctatg gatggcactg tatatatgcg atggaaaacg gtgcttcctc tgtagtaggt
     6061 gttgatattt ctcataaaat gctcgaagta gcaaaaggaa aaacccattt tccacagatt
     6121 gaatatgaat gctgtgccat agaagatgtg gatttcccag aggagagctt tgatgtaata
     6181 ctaagttcgc ttgcgtttca ttatgtagca gactatgaga atttaataaa aaagatatat
     6241 aggatgctga aggctggtgg caatttagtt tttacagttg aacatcctgt ttttactgct
     6301 catggaacac aagactggta ttataacgaa aaaggagaaa tactgcattt cccggtggac
     6361 aattattatt atgagggcaa acggacagct atgtttttgg aagaaaaggt tacaaaatat
     6421 catagaacac tgaccacata tctaaataca ctgctttcaa atagttttat aataaatcag
     6481 attgtggagc cacagccgcc agagaacatg atggatattc cggggatggc ggatgaaatg
     6541 cgacgcccaa tgatgctgat tgtatcggca aaaaagaaga tgtaataata tagaaaaaat
     6601 aaacgaggag tatgtaaatg agatcagaaa aagaaatgat ggatttagta ctttctttag
     6661 cagaacagga tgaacgtatt cgaattgtga cccttgaggg gtcacgcgca aatattaata
     6721 tacctaaaga tgaatttcag gattatgata ttacatattt tgtaagtgat atagaaccgt
     6781 ttatatctaa tgatgactgg cttaatcaat ttgggaatat aataatgatg caaaagccgg
     6841 aggatatgga attattccca cctgaagaaa agggattttc ctatcttatg ctatttgatg
     6901 attacaataa aattgatctt accttattgc ccttggaaga gttagataat tacctaaagg
     6961 gcgataaatt aataaaggtt ctaattgata aagattgtag aattaaaagg gacatagttc
     7021 cgactgatat agattatcat gtaagaaagc caagcgcaag ggagtatgat gattgctgca
     7081 atgaattttg gaatgtaaca ccttatgtta ttaaaggatt gtgccgtaag gaaattttat
     7141 ttgctattga tcattttaat cagattgttc gccatgagct gctgagaatg atatcatgga
     7201 aggtcggcat cgaaacaggc tttaaattaa gtgtaggcaa gaactataag tttattgaaa
     7261 ggtatatatc cgaggatttg tgggagaaac ttttgtccac ctaccggatg gattcctatg
     7321 aaaacatatg ggaagcatta tttctatgcc atcaattgtt cagggcggta tccggtgagg
     7381 tggcggaaag gcttcattat gcctatccgg agtatgatag gaatataaca aaatatacca
     7441 gggacatgta taaaaaatac actggtaaaa ccggctgcct ggatagcaca tatgccgctg
     7501 atatagaaga gaggcgggaa cagtgattac agaaatgaaa gcagggcacc tgaaagatat
     7561 cgataaaccc agcgaaccat ttgaggtgat aggtaagatt ataccgaggt atgaaaacga
     7621 gaattggacc tttacagaat tactctatga agcgccatat ttaaaaagct accaagacga
     7681 agaggatgaa gaggatgagg aggcagattg ccttgaatat attgacaata ctgataagat
     7741 aatatatctt tactaccaag acgataaatg cgtcggaaaa gttaaactgc gaaaaaattg
     7801 gaaccggtac gcttatatag aagatatcgc cgtatgtaag gatttcaggg ggcaaggcat
     7861 aggcagcgcg cttatcaata tatctataga atgggcaaag cataaaaact tgcatggact
     7921 aatgcttgaa acccaggaca ataaccttat agcttgtaaa ttctatcata attgtggttt
     7981 caaaatcggc tccgtcgata ctatgttata cgccaacttt gaaaacaact ttgaaaaagc
     8041 tgttttctgg tatttaaggt tttagaatgc aaggaacagt gaattggagt tcgtcttgtt
     8101 ataattagct tcttggggta tctttaaata ctgtagaaaa gaggaaggaa ataataaatg
     8161 gctaaaatga gaatatcacc ggaattgaaa aaactgatcg aaaaataccg ctgcgtaaaa
     8221 gatacggaag gaatgtctcc tgctaaggta tataagctgg tgggagaaaa tgaaaaccta
     8281 tatttaaaaa tgacggacag ccggtataaa gggaccacct atgatgtgga acgggaaaag
     8341 gacatgatgc tatggctgga aggaaagctg cctgttccaa aggtcctgca ctttgaacgg
     8401 catgatggct ggagcaatct gctcatgagt gaggccgatg gcgtcctttg ctcggaagag
     8461 tatgaagatg aacaaagccc tgaaaagatt atcgagctgt atgcggagtg catcaggctc
     8521 tttcactcca tcgacatatc ggattgtccc tatacgaata gcttagacag ccgcttagcc
     8581 gaattggatt acttactgaa taacgatctg gccgatgtgg attgcgaaaa ctgggaagaa
     8641 gacactccat ttaaagatcc gcgcgagctg tatgattttt taaagacgga aaagcccgaa
     8701 gaggaacttg tcttttccca cggcgacctg ggagacagca acatctttgt gaaagatggc
     8761 aaagtaagtg gctttattga tcttgggaga agcggcaggg cggacaagtg gtatgacatt
     8821 gccttctgcg tccggtcgat cagggaggat atcggggaag aacagtatgt cgagctattt
     8881 tttgacttac tggggatcaa gcctgattgg gagaaaataa aatattatat tttactggat
     8941 gaattgtttt agtacctaga tttagatgtc taaaaagctt taactacaag ctttttagac
     9001 atctaatctt ttctgaagta catccgcaac tgtccatact ctgatgtttt atatcttttc
     9061 taaaagttcg ctagatagag ttctatatta gagatgtaaa gagttttggt gaagaggttt
     9121 gtgagtatgg cttttataac cagggtgcaa tgagaagcac taacagcagc tagcttaaga
     9181 acgcatagga tatacttttt atggaagtca cagaggagga ataaaatttg aacaggatta
     9241 acagagtaac ctctattctt attcagctgc aatcaaaaaa aataattcct gccaaagaaa
     9301 ttgcacagcg atttaatata agcttaagga cagtttacag agatatccgg acacttgaag
     9361 aagccggaat accaattgga tctgaggccg gaaaaggata ttttcttgta gagggcttcc
     9421 tacttccgcc ggtaatgttt acagcggcgg aagtgggtgc attgattaca gcagggaaat
     9481 ttttaaattg ccatggagat gaatcattta taaaggattt tgattcagct atgtataaaa
     9541 tcaaatctat tttgaagcat ggtgaaaaaa actatgcaca ggagttggag aacagtatta
     9601 acgtgtacag cacatctgga cagaaaaata cattggcgga taacgttatt gcagcaattc
     9661 agactgcaat ctgcaataaa agggtaatat caattcaata tcctgcatca ggagggcaag
     9721 aaccggaaag cagaatgata gagcctatat cactggggtt ttatgaacag aactggtact
     9781 taatcggttt tgcaggctaa gaaatgaata taggaatttt cgtgtggatc ggattaaaag
     9841 catttgcata gaagaagagc tctgtcaaag ccatgatggc tctttggaac aaatattaaa
     9901 acaaatgctt tcatataaaa aactatatgc cctacaatgc cgttgtaaaa attggggaaa
     9961 agggatatat tgattttata tctttctcaa aataaatata aaagcccttt aaagagggct
    10021 tttatatatt aatcacaaat cacttatcac aaatcacaag tgattaatca caaatcactt
    10081 gtgatttgtg attcttaatg atacaatatt actatacaaa aaaagaatgg ggcgtagtta
    10141 tggagaagga aaaactaaaa atacttgaag aattaagacg tattttaaac aataaaaatg
    10201 aagcaattat tatcttgaat aattacttta aaggtggtgt tggaaagtcc aaattatcga
    10261 ctatgtttgc ttacttgaca gacaaattga atttaaaagt tttaatgatc gataaggact
    10321 tacaggcaac attgacaaaa gacttagcaa aaacttttga ggtagaattg ccacgtgtca
    10381 atttttatga aggcttgaaa aatggaaact tggcttcttc tattattcat ttgactgata
    10441 atttagactt gatccctggc acgtttgatt tgatgttact gccaaaatta actcgctcat
    10501 ggacgtttga aaatgaaagt agattgcttg ctactctttt agcaccttta aaaagtgact
    10561 atgatcttat tattattgat actgtaccaa cgccaagcgt ttatacaaat aatgcaatcg
    10621 tggcgagtga ttacgttatg atccctttac aagcagaaga agaaagtaca aacaacattc
    10681 aaaactatat ttcctatttg attgatttgc aagaacagtt taaccctgga ctagatatga
    10741 ttggttttgt tccttattta gttgatacgg acagcgcaac gataaaatca aacctggaag
    10801 aactgtacaa agaacataaa gaggataatt tggttttccg aaatattatc aagcgaagta
    10861 ataaagtaag tacttggtct aaaaatggca ttacagaaca caaaggctat gacaaaaaag
    10921 ttttgtctat gtatgagaac gtattttttg aaatgattga gcgaatcatt caattagaaa
    10981 atgaaaaaga atagaatcac aaatcacaag tgattaatca caaatcactt gtgatttgtg
    11041 ataggtgatg ataaaataaa tagtaagaag aaatagaaag aagtgagcga tcgtgggaaa
    11101 tttaggcgca cagaaagcaa aacgaaatga tacgccaatc agcgcaaaaa aagatataat
    11161 gggggataag acggttcgtg ttcgtgccga cttgcaccat atcataaaaa tcgaaacagc
    11221 aaagaatggc ggaaacgtaa aagaagttat ggatcaagcc ttagaagaat atatacggaa
    11281 atatttacct gacaaacttt aaaaggagtg aaaatgaaat ggcagttacg tatgaaaaaa
    11341 catttgaaat tgaaatcatt aatgagttat cggcaagtgt ttataatcgg gtattaaatt
    11401 atgttttgaa tcatgaacta gatactaaaa atactcgttt actagaagtg aatcttttaa
    11461 atcaattaga agtggcacaa gaagttgatt tatttcaaca accatttgaa gaattacaag
    11521 ctattcatga gtattggcgg tcaatgaatc aatattcaaa acaaattttg aataaagaga
    11581 aagtggctta acatggcgaa tattactgac ttcaccgaaa agcaatttga agatcgttta
    11641 gaaaagaatg ttgaacgact aactaaaaat agactagcgg ttgaatcgcc aaccgctttt
    11701 ttacttggtg ggcaaccagg gtcagggaaa accagtttgc gatcagcaat ttttgaagaa
    11761 acacaaggga atgttattgt cattgataat gataccttta aacaacagca cccaaatttt
    11821 gacgaattag tgaaactcta tgaaaaagac gtagtaaaac acgttacccc ttattcgaat
    11881 cgcatgacag aagcacttat tagccgttta agcgatcaag gctataattt agtgattgaa
    11941 ggaacaggac gaacaacaga cgttcccatt caaacagcca caatgcttca atctaaaggt
    12001 tatgaaacaa aaatatacgt catggcagta ccgaaaattg aatcatactt aggaacgatt
    12061 gaacgatatg aaaccatgta tgcagatgat ccaatgacag ctagggcaac accaaaacaa
    12121 gcgcatgata ttgttgtcaa aaacttaccg accaatttag aaacccttca taaaacgggc
    12181 ttatttagcg atataaggct ttacaataga gaaggagtga aactctattc aagcttagaa
    12241 acgccttcca ttagtccgaa agagacctta gaaagagaat taaatcgtaa agtatcaggg
    12301 aaagaaattc aaccgacttt ggagagaata gagcaaaaaa tggttcaaaa tcaacaccaa
    12361 gaaacccctg aatttaaagc aattcaacaa aaaatggaaa gcttgcagct acctacacca
    12421 ccaataccca aaacacctaa acttccaggt atttaaacct taaaaaagga aaagagtagt
    12481 taccaaaaaa cggtaactac tcttttttta taaaaacatt tcctcatgtt ggggtcccga
    12541 gcgcctacga ggaatttgta tcgataagaa atagatttaa aaatttcgct gtttttttct
    12601 aatttcattt tgaggtgaat taattggtag ttattagagg tgccctatac ctaataattt
    12661 atctacattc cctttagtaa cgtgtaactt tccaaattta caaaagcgac tcatagaatt
    12721 atttcctccc gttaaataat agataactat taaaaataga caatacttgc tcataagtaa
    12781 cggtacttaa attgtttact ttggcgtgtt tcattgcttg atgaaactga tttttagtaa
    12841 acagttgacg atattctcga ttgacccatt ttgaaacaaa gtacgtatat agcttccaat
    12901 atttatctgg aacatctgtg gtatggcggg taagttttat taagacactg tttacttttg
    12961 gtttaggatg aaagcattcc gctggcagct taagcaattg ctgaatcgag acttgagtgt
    13021 gcaagagcaa ccctagtgtt cggtgaatat ccaaggtacg cttgtagaat ccttcttcaa
    13081 caatcagata gatgtcagac gcatggcttt caaaaaccac ttttttaata atttgtgtgc
    13141 ttaaatggta aggaatattc ccaacaattt tatacctctg tttgttaggg aattgaaact
    13201 gtagaatatc ttggtgaatt aaagtgacac gagtgttcag ttttaatttt tctgacgata
    13261 agttgaatag atgactgtct aattcaatag acgttacctg tttacttatt ttagccagtt
    13321 tcgtcgttaa atgcccttta cctgttccaa tttcgtaaac ggtatcggtt tcttttaaat
    13381 tcaattgttt tattatttgg ttgagtactt tttcactcgt taaaaagttt tgagaatatt
    13441 ttatattttt gttcatgtaa tcactcctga agtgattaca tctataaata aatacagaag
    13501 ttaaacgatt tgtttgtaat tttagttatc tgtttaaaaa gtcataagat tagtcactgg
    13561 taggaattaa tctaacgtat ttatttatct gcgtaatcac tgtttttagt ctgtttcaaa
    13621 acggtagatg ttttatctac attacgcatt tggaatacca acatgacgaa tccctccttc
    13681 ttaattacaa atttttagca tctaatttaa cttcaattcc tattatacaa aattt
//
LOCUS       DASHHB010000064        13721 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.066, whole genome shotgun sequence.
ACCESSION   DASHHB010000064 DASHHB010000000
VERSION     DASHHB010000064.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13721)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13721)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13721
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.066"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..914)
                     /locus_tag="U2K71_002311"
     CDS             complement(<1..914)
                     /locus_tag="U2K71_002311"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_042964654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase family protein"
                     /protein_id="HEM7316340.1"
                     /translation="MIISTITEAIGSTPLYRFDNQHYSMPKGSAIYAKLEYLNPGGSI
                     KDRLGSYLIKEGFAQGKINEKTTIIEPTAGNTGIGIALAALTYKLRTVFVVPEKFSLE
                     KQQLMKALGAKIIHSPSEQGIIGAISKSKKLAEEISNSYLPLQFENKDNPAAYYHTLG
                     PEIFHEIKENIHSFVAGIGSGGTFAGTSTFLKEKYPDIRIIGVEPEGSVLNGGDPAPH
                     EIEGIGVEFIPPFLSPLSINQIETISDVEGFNYTRQLAREQGLLVGSSSGAAFAAALR
                     EIRRLPPGHRVVTIFPDAADRYLSKNIY"
     gene            1342..1905
                     /gene="efp"
                     /locus_tag="U2K71_002312"
     CDS             1342..1905
                     /gene="efp"
                     /locus_tag="U2K71_002312"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HEM7316341.1"
                     /translation="MISASDLRAGMTFVQDGKLIKVLDASHHKPGKGNTVMRMKLKDV
                     RSGATYDTTFRPEEKFEKAHIDTKPVQYLYTMDDTAFFMDLETYEQYEIPVETVEEEM
                     KYILENMEVKIQFFGSEVIGVQLPTTVVLRVVETQPSIKGATVTGSGKPATMETGLVV
                     NVPDFIETDELLEINTAEGSYVKRASK"
     gene            2006..2176
                     /locus_tag="U2K71_002313"
     CDS             2006..2176
                     /locus_tag="U2K71_002313"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316342.1"
                     /translation="MKNKKVFDFIYILKDEWQKLEKEKTALIYEEIEMIHKIENIRTI
                     PNYIEAKIFFHH"
     gene            2391..3650
                     /gene="lysA"
                     /locus_tag="U2K71_002314"
     CDS             2391..3650
                     /gene="lysA"
                     /locus_tag="U2K71_002314"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HEM7316343.1"
                     /translation="MTELIIGGVTASTLAEKYKTPLYVYDEEKMRQTMLSFKRGFVSK
                     RFDTKVLYASKAFQTIEMLHLVREYGFGLDVVSGGEIYTALQADFPRDTIYFHGNNKT
                     PEELIYALENDLLHIVADNLMEVELLAELSQRYLKKICIMLRLNVGVEAHTHEYIVTA
                     HIDSKFGMSYESKECQQALQLIKESSYLELEGFHAHIGSQIFDVTAWLAEIDKLTNYL
                     TDFDQPLSLNLGGGFGIRYTQNDQPIPVEEALKKLIEYTEEALEKRKLTIRQLLIEPG
                     RSIVGEAGTTLYTIGFIKKTPHKKYYFVDGGMTDNIRPALYQAEYDCDLAAKLSEEKT
                     EKVTIAGKMCESGDVVIKEAYLPKAESGDLLAVYSTGAYGYSMSSNYNRATRPAVVFV
                     NNGQSRLIVRRQAFSDLLRGEVSYDPS"
     gene            3637..4437
                     /gene="dapF"
                     /locus_tag="U2K71_002315"
     CDS             3637..4437
                     /gene="dapF"
                     /locus_tag="U2K71_002315"
                     /EC_number="5.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HEM7316344.1"
                     /translation="MILHKYNGCGNDFILLDYAEDTDYSQLAASLCTKEKYDIDGLIA
                     VKIDPLEMIYYNKDGSRAPMCGNGIRCFARYVNDQNMMNEQEFNVETLAGTRKITVLD
                     PSPFYCSVDMGVPDDSPEKIKANQTYPIKNQEIEINGEKVTITSLFLGTIHTVVFVEN
                     AREEVWRKRGELICHHPLFKEKTNVNFVEVVNEQELIVRTYERGVGWTLACGTGCCAS
                     YVVAKETGKISADQVVVHLEQGDLMISGEDAIQMAGPAVHEWTRILEE"
     gene            4440..5321
                     /locus_tag="U2K71_002316"
     CDS             4440..5321
                     /locus_tag="U2K71_002316"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HEM7316345.1"
                     /translation="MIKGSIVALITPMNEEGSVDYAGLEKLIQFHLDEQTDGLLVLGT
                     TGESSTLTQSEEEQILQLTVKKVAGRVPVIAGAGTNNTKETIEKAKHFASLGADALLV
                     ITPYYNKTSDAGLAAHFTAIAEASPIPLILYNVPSRTGMSIPIHVLVNLAEHPNIIGL
                     KEASGDMAYVMDAARLIGEEFFLYSGNDDLILPVMSVGGSGVISVWANIQPKIVHELV
                     KDTQDGRWQQAKEKQLNALELIHALFSETNPIPVKAAMSLLDLPSGPLRLPLVSLSKE
                     KKKQLAQLLLKERTVEK"
     gene            5318..6034
                     /locus_tag="U2K71_002317"
     CDS             5318..6034
                     /locus_tag="U2K71_002317"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HEM7316346.1"
                     /translation="MKIGLIGSGQMGNRVEEVAQERGDTVLLLRTAPKEATTLAFTEL
                     PELLIDFSHPDNLEKILSYSLSYQLPLVIGTTGYTQSQFQEIKEHAKHVPVMYSANFS
                     FGIMVMNQLIKQAAAMLQGWQIELIEKHHSRKKDAPSGTANMLLQTIQEVQKLQPVYN
                     WQGKKREENQIGVHSIRAGSLPGEHDVLFAATDEIFTIKHESFSNRIFAAGAVEAADW
                     LKDQSPGYYKLEDLLMDKGV"
     gene            6040..6735
                     /gene="dapD"
                     /locus_tag="U2K71_002318"
     CDS             6040..6735
                     /gene="dapD"
                     /locus_tag="U2K71_002318"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-acetyltransferase"
                     /protein_id="HEM7316347.1"
                     /translation="MEAQEIIRYIQTASKKTPVKVYLNVTEPIEFKESKVFGEGSSFV
                     VFGDYETIAPVLEAESEKIIEIEIETAARYSAVPLLDIKKINARIEPGAIIRDQVSIG
                     NNAVIMMGAIINIGAVIGENTMIDMGAVLGGRATVGKNCHIGAGAVLAGVIEPASAKP
                     VIVEDGVLVGANAVIVEGVHIGKDAVVAAGAVVLEDVAAETVVGGIPARVLKISDDQT
                     KENTALIAALREL"
     gene            6751..7752
                     /locus_tag="U2K71_002319"
     CDS             6751..7752
                     /locus_tag="U2K71_002319"
                     /EC_number="1.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate-semialdehyde dehydrogenase"
                     /protein_id="HEM7316348.1"
                     /translation="MREIDTCIVGATGLVGQTMLKVLEEYDFPVGRLKLLASKRSAGK
                     VCVFKKKKHTVQELTETSFEGYDLALFSAGASVSKEFAPIARSQGLFVIDNSSAWRED
                     SDIPLIVPEVNLEDYTLNRLIANPNCSTIQAVLPLKALQEAFGIQRVNYSTYQAVSGS
                     GQKGIEDLHRTQNDEEPVLYPYNISQTVIPEIDQPLENGYTKEEQKMINETRKILHQP
                     ALPVSATCVRVPIENGHGVSVAVQLQKEFTTEEIRECLSQFPGIQLEDDLAAHKYPTS
                     ILARGTDMVYVGRIRKDTSLENGLLFYTTADNIRKGAAANAVQIAAALMQKIAEVAR"
     gene            7749..9110
                     /locus_tag="U2K71_002320"
     CDS             7749..9110
                     /locus_tag="U2K71_002320"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HEM7316349.1"
                     /translation="MSNTLKVAKFGGTSMSHEEQFKKVRTIVLSDPNRQVIVVSALGK
                     RTSDDDKVTDLLYLIYAYLQHHVDWTPLWKRICDRFIQVRDVLALQLPIEDELARIQK
                     QLEDEKITEDYLVSRGEYLTAQLMAEYLGYQFIDAKDLIFFDYDGKIDQEKTAQEITK
                     AFKRYPKMVVPGFYGANPAGKEKLLGRGGSDITGAILAKMLQAESYENWTDVSGIMMA
                     DPRIIDHPKIIEEISFRELREMAYMGANVLHEAAVFPVQEADIPIQIKNTNDPFAQGT
                     KISNHEIEKENGLTGIAGRKDFLSITLFKRHMSDEIGFIWKAMSIFAKHEISIEHIPS
                     GIDNIGVIVSAEAIADKLFLITKELKETLGVEEIVVIEDLALISVVGGPHKELIGLSG
                     KVLSILNKLEIRTSILSQGAQELNLIIGVPNNQYETVVKGIYEGMVNTDAERISNGTP
                     SNS"
     gene            9070..10221
                     /locus_tag="U2K71_002321"
     CDS             9070..10221
                     /locus_tag="U2K71_002321"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HEM7316350.1"
                     /translation="MLKEYRMALHQIPEIGFHEFKTKKYLYEQVKNSEAVIHEIGETG
                     LLLYFDNQQEETIAFRTDIDALPVKEETNLSFASKHEGYMHACGHDGHMAMLLGLTDF
                     ITKHIEDLIYNVVLIYQPSEEISGGAQSVIQSGLLDKYEVQAIFGFHLWPGLAKGEVF
                     SRPGPLMAQSSETDIIIKGQSAHIASSEKGIDSLEAAVRFLKNVYDFDRQLPAEWNHL
                     MKFGQIQGGTIRNVLAGEVIVSGSIRSYSSDMQNYLKRGLDHLAEQFHSTATISFRYN
                     DGYPAVINHRGLYAALLDSNKLHELDAPVLQAEDFGIYTQQYPCVFFFLGVGDTPALH
                     DPTFDFDMDVLENGVEWYKTILFTENLLVKIDSSSKEERVNNDEFIQSI"
     gene            10196..11365
                     /locus_tag="U2K71_002322"
     CDS             10196..11365
                     /locus_tag="U2K71_002322"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM7316351.1"
                     /translation="MMNLSNRFSPRLSRIEVSKIRQFDQQISSIPDVIKLTLGEPDFP
                     TPEHVKQAGIAAIEEDFSHYTGMRGLEELREAACIFQQQRYGLTYDPQTEVLTTVGAT
                     EAIASALLSVLEEGDKVLIPAPAYSGYQPLVELAGAELIPIDTSDTGFVCQPEQFERA
                     FEQYGSAVKAVILNYPNNPTGTTLSAKQLKAIAEVLKKYPVFVISDEVYAELTYSGTH
                     MSIATYLPEQTIVISGLSKSHAMTGWRVGFIFAQKALIDELIKVHQYLVTAATTMSQK
                     AAAEALINGVKDSENMKEQYQTRRDYLVQQLAPLGFEVTQPNGAFYLFCKLPAAIQTD
                     SWQFCVELAEQAKVACIPGIAFGPEGEGYIRISYASSMEKLHEACKRIKDFLTEQ"
     gene            11540..12919
                     /locus_tag="U2K71_002323"
     CDS             11540..12919
                     /locus_tag="U2K71_002323"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM7316352.1"
                     /translation="MEKIKVVIVGASHGGHQSILELLTRYGENVDITLFEAGDYISFM
                     SCGMELYLEDKVTDVNDVRNFRPENFPQSNVHILNNHEVKTIDADKKTVTVTSASDNE
                     TKEYPYDKLILSSGVKPNNLPVPGADLENVYLMRGYNWATKIKEKLNDPAIKKIAVVG
                     AGYIGIEAAEASRKAGKEVVLLDVIDRPLGTYLDAEMTDILEQHLNENGIEVLTNAKI
                     EAFTGNGKVEAIKTSEKEIPADLVIQAAGVKANTEWLKGIVDLDERGWIQTNEYLQTN
                     LPDVYAVGDATLAYSIPARKKMPIALATVARREARYVVKHLFEEVPNKPFGGVVGSSA
                     LSVFDYHFAASGLNSFTAAKAGVDVRVSYYEDTLRPKYVPEEQGNPKVSIQLFSDPMT
                     HQLLGGAVLSTYDVTGQGNVLALAIQNKLTLEELAEADFFFQPGFDRQWSMLNLAAQQ
                     ALGEEPFVE"
     gene            13200..13721
                     /locus_tag="U2K71_002324"
                     /pseudo
     CDS             13200..13721
                     /locus_tag="U2K71_002324"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         4575 a         2399 c         2984 g         3763 t
ORIGIN      
        1 aagtaaatgt tttttgataa atagcggtct gctgcgtctg gaaaaatcgt aacgacacga
       61 tgaccaggtg gtaaacgacg aatttctctt aatgctgctg caaaagccgc accactcgaa
      121 cttccaacta atagtccttg ttctcgtgct aactgcctag tatagttaaa cccttctaca
      181 tcagagattg tctcgatctg attgattgaa aggggagaaa gaaacggcgg aatgaattct
      241 acaccgatac cctcaatttc gtgaggggct ggatcaccac catttaacac agagccctct
      301 ggttctacac cgattattcg aatgtcggga tatttttctt tcaaaaaggt agaagttcca
      361 gcaaaggttc caccactccc aattcctgct acaaaactat ggatattttc tttgatttca
      421 tgaaagattt ctggccctaa cgtatgatag taagcagcag gattatcttt attttcaaat
      481 tgaagaggca gatagctgtt tgatatctcc tctgcaagtt ttttactttt gctgatggca
      541 ccgatgattc cttgctcact aggagaatgg atgatctttg ctcccaatgc tttcatcaat
      601 tgttgttttt ctaagctaaa cttctctgga acaacaaaaa ccgttcttaa tttgtatgtg
      661 agtgcggcca gagcgatccc aattcctgta tttccggcag ttggttcgat gatcgtcgtt
      721 ttttcattga tttttccttg ggcaaatcct tctttgatca aataactgcc caatctatct
      781 ttgatacttc ctccaggatt taaatattct agttttgcat aaatagcaga acctttaggc
      841 atagaatagt gctgattgtc aaagcgataa agcggtgtgg aaccaatagc ttctgtaatt
      901 gtcgagataa tcatgtgggc ctcctaaaaa aagataataa aaaaaggagc tgactattaa
      961 atcagctcct tgaaagacat agagaaaaat tgagtagata aaaagataag ttacctggta
     1021 ctcattctat gaagggcaat taatagacat gacaaatagt atgctgcttt tatgcaccat
     1081 acaacagcaa cgattcaatt gtttttggtt gctgactgtc atgtgtatcc ctccaagtaa
     1141 aatttttatt aacgatagca tgaaaaaaaa taaaagtcaa aggcttgaaa gaaaaaaata
     1201 tttcgatagc atcgtttacc cgtgtttatt tttctgacaa ataggcatga gaccttaaaa
     1261 gatagagaat actctagttt tctggccggt tgtctgatac aataaagttg attatgataa
     1321 agaaaagaag gagcaaaaat aatgatctca gcaagtgatt taagagccgg tatgactttc
     1381 gtacaagacg gcaaactgat taaagttctg gatgcaagcc accataaacc tggtaaagga
     1441 aatacagtta tgcgtatgaa actgaaagat gtccgttcag gtgcaacata cgacacaacg
     1501 ttcagaccag aagaaaaatt tgaaaaagca catatcgaca caaaacctgt acaatatttg
     1561 tacacgatgg atgatacagc tttcttcatg gatttggaaa catatgagca atatgaaatt
     1621 cctgtagaaa cagtagaaga agaaatgaaa tatatcctag aaaacatgga agtaaaaatc
     1681 caatttttcg gtagcgaagt catcggcgtt caattaccaa caactgtcgt attacgtgtt
     1741 gtggaaacac aaccttccat caaaggtgca acagtcactg gttcaggtaa gccagcgaca
     1801 atggaaactg gcttagtagt caatgttcct gacttcatcg aaacagatga attgttggaa
     1861 atcaatacag cagaaggttc ttatgtaaaa cgtgcgtcaa aataattcgc taacggattg
     1921 ataagaattt gaaaacagag cttgacctct tgaaaggcag atcatcggat ctgcctttca
     1981 agaggttttt tgtggaatca gcaaattgaa aaataaaaaa gtatttgatt ttatttatat
     2041 tttgaaagat gaatggcaaa agctagagaa agaaaaaact gcacttattt atgaagaaat
     2101 tgaaatgatt cataaaattg aaaatatccg aactattccg aactatattg aagcgaaaat
     2161 attttttcat cactaaatag tgttgaattt atgtttttat gtataaatag cttctaatag
     2221 ttcgggtatt ttgtttttat aaccattttt attcttattt ttaaaaaaat atttcgaaat
     2281 atactagaca attcagaaaa ttttgtttaa tattattcca ataaaattca aatctttatc
     2341 aaatgaaatt ccaataaaaa cggaaaaaaa gtgaaaagag gaatgaaaaa atgacagaat
     2401 tgataattgg cggagtcaca gcaagcacat tggcagaaaa gtataagaca cctctgtatg
     2461 tgtatgatga agaaaagatg agacaaacga tgctgtcatt caagcgaggc tttgtatcga
     2521 aacgttttga tacaaaagtg ctttacgcct ctaaagcatt ccaaacgatt gagatgctgc
     2581 atttagttcg tgaatatgga ttcgggctag atgttgtcag tggtggggaa atctataccg
     2641 ctttgcaagc tgattttcct agagatacta tctacttcca tggtaacaac aagacgccag
     2701 aggaactgat ctatgcactt gaaaatgatt tgttgcatat tgttgccgat aatttgatgg
     2761 aagttgaatt attagcagaa ttaagccaac gctatttaaa aaaaatctgt atcatgcttc
     2821 ggttgaatgt cggagtagaa gcacatactc atgaatatat cgtcactgcg catattgatt
     2881 caaaattcgg catgagctat gaaagtaaag agtgccaaca agcccttcag ttgataaaag
     2941 aaagcagcta tctagagtta gaaggatttc atgctcatat cgggtcacaa atttttgatg
     3001 ttactgcttg gttagctgaa atcgacaaat tgacgaatta cttaaccgat tttgaccaac
     3061 ctctttcctt gaatcttggc ggagggtttg ggattcgtta tacacaaaat gatcagccga
     3121 tcccagtcga agaggcacta aaaaaactga ttgaatatac ggaagaagct ttggaaaaac
     3181 gaaaactgac cattcgtcaa ttactgatcg aaccgggtag aagtattgtt ggagaagctg
     3241 gtacaacact atacacgatt ggtttcatca aaaagacacc acataaaaaa tattactttg
     3301 tagatggcgg gatgacagat aatatccgtc cagctctgta tcaggcagaa tacgattgtg
     3361 atttagcagc aaaactgtcg gaagaaaaaa cagagaaagt gacgattgca ggaaaaatgt
     3421 gtgaatcagg cgatgtggta attaaagaag cttatttgcc gaaagcagaa tcgggtgatc
     3481 ttttagctgt ttattccacg ggagcttatg gatactcgat gagcagcaac tacaatcgag
     3541 cgacacgacc agcagttgtt tttgtcaata atggccaatc aagactgatt gttcgcagac
     3601 aagcattttc tgatctacta aggggggaag tttcctatga tccttcataa atataatggc
     3661 tgcggaaatg attttatctt attagattac gcagaagaca cagattatag tcaattagcc
     3721 gcttctttat gcacaaaaga aaaatatgat atagacggat tgatcgccgt aaaaatcgat
     3781 ccgttggaaa tgatctatta caacaaagat ggcagccgcg cgccgatgtg cggaaatggc
     3841 attcgctgtt ttgctcgata cgtaaatgat cagaacatga tgaatgaaca ggaattcaac
     3901 gtagagacat tagcaggcac aagaaaaatt acggtgttgg atccctcacc tttttattgc
     3961 agcgtagata tgggagtgcc agatgattct ccggaaaaaa tcaaagctaa tcaaacttat
     4021 ccaataaaaa accaggaaat agagatcaat ggtgaaaaag taacgatcac tagtttattt
     4081 ttaggaacga ttcataccgt tgtttttgtt gaaaatgcgc gtgaggaagt ctggagaaaa
     4141 agaggtgaac tgatctgtca tcatcctttg ttcaaagaaa aaaccaatgt caactttgta
     4201 gaagtagtca atgaacaaga attgatcgtc cgtacctatg aaagaggagt aggttggaca
     4261 cttgcttgcg gtacaggttg ctgtgcctct tatgtggttg ctaaggaaac tgggaaaatt
     4321 tcagcagatc aggtagttgt tcacttggaa caaggagact tgatgatctc tggtgaggat
     4381 gcgatacaaa tggctggccc agcagttcat gaatggacaa gaatattgga ggaatgaaga
     4441 tgataaaagg ttcgatcgtg gcgctgatca cgccgatgaa tgaagaggga tcagtagatt
     4501 atgctggttt ggaaaaattg atccaattcc atttagatga acaaacagat ggattattag
     4561 ttttaggtac aacaggagaa tcttctacgc tcacacaatc tgaggaggag cagatccttc
     4621 aactgacagt aaagaaagtg gctggccgtg tgccggtgat tgcaggagct ggaacgaata
     4681 atacgaaaga aacgattgaa aaagcgaagc attttgcttc tttgggggca gacgcacttt
     4741 tggtcatcac cccttactac aacaaaacaa gtgatgcagg actagctgct cattttacag
     4801 caattgcaga agcctcaccg attccgctta ttttgtataa tgttccttct agaacaggca
     4861 tgtcgattcc aatccatgta ttagtaaatt tagcagaaca tcctaatatc attggtttga
     4921 aagaagcctc aggagatatg gcttatgtga tggatgccgc tcggttgatt ggagaagaat
     4981 tcttccttta cagtggaaat gatgatttga tcctaccagt aatgagtgta ggaggaagcg
     5041 gtgtaataag tgtatgggcg aatatccaac ctaaaatagt ccatgaatta gtaaaagata
     5101 cgcaggacgg aagatggcag caagcaaaag aaaagcaatt aaatgcactt gaactgatcc
     5161 atgccttgtt cagtgagacc aatcctattc ctgtcaaagc agccatgtca ttattagact
     5221 taccttccgg acctctacgc ctgcctttag tttctctttc aaaagagaaa aagaaacaat
     5281 tagctcagct tttgttaaaa gaaaggacgg ttgaaaaatg aaaatcggac tgattggcag
     5341 tggacagatg ggaaatagag tcgaagaagt tgcacaagaa agaggagata cggtccttct
     5401 tttacggacg gcacctaaag aagcaactac gcttgctttt acggaactgc cagaactgct
     5461 tattgatttt tctcatccag ataatttaga gaaaatcctt tcgtatagct tatcatatca
     5521 acttccgtta gtcattggca cgacaggtta tacacaatca cagttccaag aaataaaaga
     5581 acatgccaaa catgtacctg tgatgtatag tgctaatttt tcgttcggca tcatggtgat
     5641 gaatcaacta atcaagcaag cagctgcaat gttgcaaggc tggcagatcg aactgattga
     5701 aaaacatcat agtcgtaaaa aagatgcacc atctggtact gcaaatatgc tgcttcaaac
     5761 aatccaagaa gtgcaaaagc tgcagcccgt atacaactgg caagggaaaa aaagagaaga
     5821 aaaccaaatt ggtgtacaca gtattcgtgc aggttcttta cctggtgaac atgatgtatt
     5881 gtttgctgct actgatgaaa tatttacgat caaacatgaa tctttttcta atcgaatctt
     5941 tgctgcaggc gcagtagaag cagcggactg gttgaaagat cagtcaccag gttattacaa
     6001 attagaagat ttattaatgg acaaaggggt gtaaaaaacg tggaagctca agaaatcatc
     6061 cgctacattc aaacggcatc taaaaagacg cctgtgaaag tctatctgaa tgtaaccgag
     6121 ccaatcgaat ttaaagaaag taaagttttt ggtgaaggaa gcagcttcgt tgtttttggt
     6181 gactatgaga cgattgctcc ggttttagaa gctgaatcag aaaaaatcat tgaaatcgaa
     6241 atcgaaactg ctgcaagata cagcgcagtt cctttgctag atattaaaaa aattaatgct
     6301 cgtatcgaac caggtgctat tatcagagat caagtgtcaa tcggcaacaa tgccgtgatc
     6361 atgatgggag cgattatcaa tattggtgca gtaatcggtg aaaacacgat gattgatatg
     6421 ggtgctgtct taggtggacg agcgacagtg ggcaaaaact gccatattgg tgcaggagct
     6481 gtcttagcag gtgtgatcga accagcatcg gccaaaccgg ttatcgtaga agacggcgta
     6541 ctggtaggtg cgaatgcagt gattgttgaa ggtgtccata tcggaaaaga tgcagtcgtg
     6601 gcagcaggag cagtggtttt agaagatgtg gcagcagaaa ctgttgtcgg tggaatacct
     6661 gcacgtgtat taaaaataag tgatgatcag acaaaagaaa atacagcatt gattgctgcg
     6721 ttacgcgaat tatagaggag atggagaaaa atgcgcgaaa tagatacctg tattgtgggg
     6781 gcaaccggat tagtcggcca aacgatgctc aaagtgttag aagaatatga ttttccagta
     6841 ggacgattga aattattagc ttcaaaacgt tcagccggta aagtttgtgt attcaagaaa
     6901 aagaaacaca ctgtacagga attgacagaa acttcatttg aaggatatga tttagcgcta
     6961 ttttctgcag gtgcttctgt atccaaagag tttgcgccaa tcgcacgaag tcagggacta
     7021 tttgtcatag ataattcctc agcatggcgg gaagattcgg atattccatt aatcgttcca
     7081 gaggtcaatc tggaagacta tacattgaat cggctgattg caaatccaaa ttgttcaacc
     7141 attcaagcgg tacttccgct taaagcttta caagaagcct ttggtatcca gcgagtcaat
     7201 tattcgacct atcaagcagt ttctggttcc gggcaaaaag gcattgaaga tcttcatcgt
     7261 acgcaaaatg acgaggaacc agttttgtat ccttataata ttagccaaac ggtgattccg
     7321 gaaatcgatc agcctttgga aaatgggtat acaaaagaag aacagaaaat gataaatgag
     7381 acccgtaaga ttctgcatca gcctgctttg ccagtatcag caacctgcgt acgtgtgcca
     7441 atcgaaaatg gacatggagt ctctgtggca gttcaactgc aaaaagagtt tacgacggaa
     7501 gaaattagag agtgtctcag ccagtttcca gggattcaat tagaagatga tctagcagca
     7561 cataaatatc cgacgagcat tcttgctaga ggaacagata tggtttatgt tgggaggatc
     7621 agaaaagaca cttctttaga gaacggattg ttgttctata cgactgccga taacatacgc
     7681 aaaggagccg ctgcgaatgc cgtacagatt gctgcagctt taatgcagaa aatagcggag
     7741 gtggcaagat gagtaatacg ctgaaagtag cgaaattcgg cggaacatcg atgtctcatg
     7801 aagagcaatt caaaaaagtc cgtacaattg ttctttcaga tcccaatcgc caagtgatcg
     7861 ttgtttctgc tttaggcaaa cgtacgtcag atgatgacaa agtaacagac ttactttatt
     7921 tgatttatgc ttaccttcag catcatgttg attggacacc cctttggaaa aggatttgtg
     7981 atcgtttcat tcaagtaaga gatgttcttg ccctgcagtt acctattgaa gatgaactag
     8041 cacgcataca gaaacaactg gaagacgaaa agataactga agattattta gtaagtagag
     8101 gtgaatatct gactgctcaa ctgatggctg agtatcttgg ttaccagttt attgacgcaa
     8161 aagacctgat tttctttgac tatgatggga aaatcgatca ggaaaaaaca gcacaggaaa
     8221 tcacaaaagc atttaaaaga tatccgaaaa tggttgttcc agggttttac ggggcgaacc
     8281 ctgcaggaaa agaaaaatta ttgggccgtg ggggaagcga cattaccggc gcgatactgg
     8341 cgaagatgtt gcaagcggaa agttatgaga attggacgga tgtttcagga atcatgatgg
     8401 ctgatccgcg aatcatcgat catcctaaaa tcatcgagga aatctctttt agagaactga
     8461 gagagatggc ttatatgggg gctaacgttc ttcatgaagc agctgttttc ccagttcaag
     8521 aggctgatat ccccatccaa ataaaaaata ccaatgatcc tttcgctcaa gggacaaaaa
     8581 tatcgaatca cgaaatagaa aaggaaaatg gactgacagg aatagctgga cgcaaggatt
     8641 ttctttccat cactttattc aaacgtcata tgtccgatga aatcgggttt atctggaaag
     8701 caatgagcat ttttgctaaa catgagatat caatcgaaca tattccttca gggatcgaca
     8761 atatcggagt gatcgtctca gcagaagcga ttgccgataa gttatttctc atcacgaaag
     8821 aattaaaaga aacacttggc gtggaagaaa tcgtcgtgat cgaagactta gctttgatct
     8881 ctgtcgttgg gggtccgcat aaagaattga ttggattatc tgggaaagtg ttatcgatat
     8941 tgaacaaatt agaaattcgc acatcgatct tatcacaagg agcacaggag ttgaatctga
     9001 tcatcggtgt accgaataat caatatgaaa cagtagtgaa gggtatctat gaagggatgg
     9061 tgaatacgga tgctgaaaga atatcgaatg gcactccatc aaattcctga aatcgggttt
     9121 catgaattca agacaaaaaa atatttatat gaacaagtaa aaaacagcga ggccgttatc
     9181 catgaaatcg gcgaaacagg actactcctt tattttgata atcagcaaga agaaacgatt
     9241 gcttttcgaa cagatatcga tgcactgcca gtcaaagaag aaacgaatct ctcttttgct
     9301 tctaagcatg aaggttatat gcatgcatgt ggtcatgacg gacatatggc gatgctttta
     9361 gggttgactg actttataac taaacacatt gaggatctta tttataatgt agtactgatc
     9421 tatcagccat cagaagaaat ttccggtggc gcacaaagcg ttattcagtc aggattgttg
     9481 gataaatatg aagtacaagc aatatttggc tttcatctat ggcctggact agctaaggga
     9541 gaagttttct ctcgaccagg acctttgatg gcacaaagca gtgaaacaga cattatcatt
     9601 aaaggccagt ctgcacatat tgcttctagt gaaaaaggaa tcgatagtct ggaggcagct
     9661 gtacgatttt tgaagaatgt ttatgatttt gaccggcaat taccggcaga gtggaatcat
     9721 ctaatgaaat ttggccaaat ccaaggtgga acgatccgaa atgttttagc tggagaagtt
     9781 attgtatccg gaagtatccg ttcttacagt tcagatatgc aaaattattt gaagagggga
     9841 ttagaccatt tagcagaaca gtttcactct acagctacta tttcttttcg ctataatgac
     9901 ggctatccag ctgtaatcaa ccatagaggc ttatatgcag cactgttaga ttcaaacaaa
     9961 ctacatgaat tagatgctcc agttcttcaa gccgaagatt ttggcatata tacgcaacaa
    10021 tatccatgtg tatttttctt tttaggtgtc ggtgataccc ctgctttaca tgatccgacg
    10081 ttcgattttg atatggatgt tttagaaaat ggtgtagaat ggtacaaaac gattcttttt
    10141 acagaaaatc ttcttgtaaa aatcgatagc agttcaaaag aggagcgagt aaataatgat
    10201 gaatttatcc aatcgattta gtcctaggct ttctagaata gaagtatcaa aaatacgaca
    10261 attcgatcag caaatatcgt cgataccaga tgtgatcaaa ctcaccctgg gagaaccgga
    10321 ttttccaact cctgaacatg tcaagcaagc aggaatagca gcgattgaag aagacttttc
    10381 tcactatacc ggaatgagag gattagagga actgcgggaa gcggcttgta tatttcagca
    10441 acagcgttat ggattaactt atgatccgca aacggaagtt ttgacgacag ttggtgcaac
    10501 ggaagcgatt gcctctgcgt tgttatctgt tttggaagaa ggagacaagg ttcttattcc
    10561 agcgcctgct tattcaggat atcagccatt agtggaacta gctggtgctg aattgatccc
    10621 gatagatact tctgacacag ggtttgtctg tcagccggaa caatttgaac gagcatttga
    10681 acaatatgga tcagctgtca aagcagtcat tttaaactat cctaacaatc ctactgggac
    10741 gacattgtct gcaaaacaat tgaaagcaat cgctgaggtt ttaaaaaaat atccggtatt
    10801 tgttataagt gatgaagtat atgctgaatt gacttatagt ggaacgcata tgtccatagc
    10861 tacttatctt ccagaacaaa caatcgtgat aagcgggtta tccaagtcac atgcgatgac
    10921 tggttggcga gtgggcttta tttttgctca aaaagccctt attgacgaac taattaaagt
    10981 ccatcaatat ctggtgactg ccgctacaac gatgtcgcaa aaagctgcgg ctgaagcgct
    11041 aataaatggg gtgaaagata gcgagaatat gaaagagcaa tatcaaacac gaagagacta
    11101 tctggtccag caattggcgc ctttaggatt tgaagtgacc cagccaaacg gtgcatttta
    11161 cttgttttgc aaactcccag ctgccattca aactgactct tggcagtttt gtgtagaatt
    11221 agcggaacag gcaaaagttg cgtgtatccc aggaattgca tttggcccag aaggagaagg
    11281 ttatatccgg atttcttatg ctagcagcat ggaaaaatta catgaggcct gcaaacggat
    11341 caaagacttt ttaacagaac agtgattcat agattttatc aaaaaaacaa tcgttaattt
    11401 tgcggtatat tctgcaagga gacgattgtt ttttaaattt agttcaaatg aacgcgtttc
    11461 ttttgattca taactaatgt aatgttaagt ttaaattgtt tagaatgatt ttaaaagaat
    11521 attcaaggag gtttcatcaa tggaaaaaat caaagttgtc attgtaggtg catctcatgg
    11581 agggcatcaa tcgatcttgg aactattgac acgatacggt gaaaatgtag atatcacgct
    11641 atttgaagca ggagactata tttccttcat gtcatgtggt atggagctgt atttagaaga
    11701 caaggtaaca gatgtaaatg atgtacgtaa tttcagacca gaaaactttc cgcaatccaa
    11761 tgttcatatt ttaaataatc acgaagtaaa aacgatcgat gccgataaaa aaacagtaac
    11821 ggttactagc gcatcagata acgaaacaaa agaatatcct tatgacaaat tgattttgag
    11881 ttctggtgtc aaaccaaaca atctgccagt accaggagca gatctagaaa atgtctactt
    11941 gatgcgtgga tacaattggg caaccaaaat caaagaaaaa ttaaatgatc cggctattaa
    12001 aaaaatcgct gtcgtaggtg cgggttacat tgggatcgaa gcagcagaag caagccgaaa
    12061 agctgggaaa gaagtcgtat tgcttgatgt gatcgataga cctttaggca cttatttaga
    12121 tgctgagatg acagatattt tagaacaaca cctaaatgaa aacggtattg aagttttgac
    12181 aaatgcaaaa atcgaagctt ttactggtaa cggaaaagtt gaggcaatca aaacaagtga
    12241 aaaagaaatc ccagcagatc tagtcattca agctgccggt gtgaaggcaa atacagaatg
    12301 gttaaaaggg atcgtcgact tagatgaacg tggctggatc caaacaaatg aatacttgca
    12361 aacgaatctt ccagatgtgt atgcagtagg agatgctaca cttgcttatt ctatcccagc
    12421 acgcaaaaaa atgccgatcg ctttagcgac tgttgcacgt agagaagcac gttatgtagt
    12481 aaaacatctt tttgaagaag taccaaacaa accgttcggc ggagtcgtag gttcctctgc
    12541 attaagtgtc tttgactatc attttgcagc aagcggattg aatagcttta cagcagcaaa
    12601 agcaggagta gatgtacgag tgtcttatta tgaagatacg ttacgaccaa aatatgtacc
    12661 agaagaacaa ggaaatccaa aagtgtccat ccaattattc tcggatccaa tgacccatca
    12721 attgctagga ggagcagtat tatctaccta tgatgttaca ggtcaaggaa atgtattggc
    12781 actagcgatc caaaacaaac tgactttaga agaactagca gaagctgact tcttcttcca
    12841 gccaggcttt gaccgacaat ggagcatgtt gaatctagcc gctcaacaag cgttaggaga
    12901 agaaccattt gtagaataag aaaacgaaat atttgaaaag aggttgtgaa aaagctgcct
    12961 tgcatcaagt aataagaaca gccaagtaaa aacagcttct tatgttttta acttggctgg
    13021 acttattacc gcagatgcaa gcttttgaac accgtttatt ttcaaaaaag ggactgtgac
    13081 acgatttgtg taccgacccc caaaagttag accaaaaaat ctaacttttg gaggtcggta
    13141 ttttttatgg ttaaatatac ttttgaactt aaattaaaga tcgttcatga ttatttggat
    13201 ggcaaaggcg attggaccgt aaaaatccag atcaagagat tgaagcggaa atgcttaaaa
    13261 ttcgggagaa gcacaaggat tatggctgtt tacggatgac caatgaacta agaaaccgcg
    13321 gtttctctat caataaaaag aaagttcagc gcttgatcaa gaagctagga ataaaagtaa
    13381 cggcatacac gcgtaaatca cgtcgctaca attcttatcg aggaaaagtt ggagcagtag
    13441 ccaaaaatcg gatccatcgt cgtttctaca ccagtatctg ccatcagaaa atcacgacgg
    13501 atacaactga atttaaatac catgaagtgg atgccaaagg cattatccgt caaaagaaat
    13561 tgtatctaga tccttttatg gatatgttca atagtgagat tttatcctat cgtatctctg
    13621 aaaagcccaa tgcattagcg gtaatggaag gtttggagga agctatccag accactaacg
    13681 attgtcccta tcgtcgtacg tttcactcag atcaaggttg g
//
LOCUS       DASHHB010000065        13597 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.067, whole genome shotgun sequence.
ACCESSION   DASHHB010000065 DASHHB010000000
VERSION     DASHHB010000065.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13597)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13597)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13597
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.067"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(115..1245)
                     /locus_tag="U2K71_002325"
     CDS             complement(115..1245)
                     /locus_tag="U2K71_002325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HEM7316353.1"
                     /translation="MGPYLTLSMIGLLEVYSASSYRLLQADENTKSLLLRQLIFIFLS
                     WSVIFLARSVKLHYLLHPKIAGYGLALSIFFLVLVRIGIFGVTVNGAQRWISLFGIQF
                     QPSELANLFLIFYLSWFFRDGNSSPKDLKKPFLITVGITFLILFQPKIAGALMILSIA
                     WVIFWAAAVPFKKGIYLIVTFSALLIGAAGGVLYLGNKGWLPQMFNHAYERIATLRDS
                     FIDSHGAGYQMTHSFYALYNGGIFGRGLGNSITKKGYLPETETDFIFSIITEELGLIG
                     ALCVLFLLFSLCMRIFCLSSRCKNQQAGLFLLGFGTLLFVQTIMNVGSIAGLMPMTGV
                     PLPFVSYGGTSYLILSLGIGITLNISSKIQAEELALYRPEKQ"
     gene            complement(1786..1992)
                     /locus_tag="U2K71_002326"
     CDS             complement(1786..1992)
                     /locus_tag="U2K71_002326"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316354.1"
                     /translation="MLKISYKPSTDSKEMKKEYETVNDFLQGQYLEVPPLQDHFVVTT
                     VTLDGKEIEMPDQTISGLFNYFNK"
     gene            complement(2008..2622)
                     /locus_tag="U2K71_002327"
     CDS             complement(2008..2622)
                     /locus_tag="U2K71_002327"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HEM7316355.1"
                     /translation="MFERSPSELTERENYKLLIGSVIPRPVAVVTTKSAKDIVNIAPF
                     SYFNIVSSNRPILSLAIQRKQDAVKDTAKNILETREAVIHILDEDNVEEVNKTAANLP
                     NEESELYLTDLTLTDSVIISVPALTEAKVRFETSLYEHVEIRNKNKVTADLFLLEVQK
                     YHLNETVYDDKTGRIDPRKLNAVSRLAGNDYAAIGEIFTIARPE"
     gene            complement(2769..3452)
                     /locus_tag="U2K71_002328"
     CDS             complement(2769..3452)
                     /locus_tag="U2K71_002328"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEM7316356.1"
                     /translation="MDTLEQIKGGVVVSCQALENEPLHSSFIMGRMALAAKEGGAVGI
                     RANTTEDINEIKRIVDLPVIGIIKRDYDGSEVFITATEKEVEELLETQAEIIALDATS
                     RKRPTEKTTAELVQMIHEHGRLAMADISTFEEALQAQEDGFDLLSTTLAGYTEYSRKT
                     QTPDFDLLNQIIEQVDIPVIMEGHTDSPEQVEKAYELGAFSVVVGSIITRPQLITKRY
                     VEAASKANN"
     gene            complement(3561..4019)
                     /locus_tag="U2K71_002329"
     CDS             complement(3561..4019)
                     /locus_tag="U2K71_002329"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASCH domain-containing protein"
                     /protein_id="HEM7316357.1"
                     /translation="MYQQVEEYVKKFQEKYPSYQSEKLGFFSFGDTEKMADELSELVV
                     KKIKTGTSSGYDLYKQENEPLPKVGQLDVVLNGKNEPVCIIKNINVTILPFSEVTKEH
                     AWKEGEGDRTLAYWRKVHAEFFKHAYKEALNIEFTEEKLVVYEEFEVIFF"
     gene            complement(4103..5866)
                     /locus_tag="U2K71_002330"
     CDS             complement(4103..5866)
                     /locus_tag="U2K71_002330"
                     /EC_number="2.2.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate synthase"
                     /protein_id="HEM7316358.1"
                     /translation="MSTVLHVLDAINSPADVKKLSVEEMKQLAEEMRQKILQKDSDVG
                     GHVGPNLGVVELTIAFHYVFDSPKDKIVWDVSHQSYGHKLLTGRKNGFEKGHYREVSG
                     YSNQHESKHDYFTVGHTSTSISLATGLAKARDLKKESGNVVAFIGDGSLSGGLAFEGL
                     NNGGVLQSNLIVLVNDNEMSIDENHGSLYKNLAELRQTNGKSSNNLFKAMGFDYRYVD
                     NGNDLETVIRLFEEVKDIDHPIVLHVHTEKGRGYQPAIDNKMKYHWHVPFDLSTGEAK
                     QVTSGETFNMIIMDYLDKKMQTSTAPIAAVNAAIPGAFGLKTFQEKHPDHYFDVGIAE
                     QHSITFASALASQGVRPVVFQSSTFLQRAYDQLSHDLAINENPVIILVGGGTISASDR
                     THQGTFDIPYLSSIPNIKYLAPATKEDMISMLDWAFEQTAQPVAIRIPTHGVEHGTAI
                     ANDYSIPAYQTVNNGGKVAILALGGFLELGKETVKAFRKKGIEATLINPGTITENDVE
                     TLDKLKETHELVITLEDGSLSGGFGENISRYYGDSSMNVLNFGAEKEFTDSVPVGQLY
                     ERYHLTPKQIVEDSLRVLNIQ"
     gene            complement(6087..7547)
                     /locus_tag="U2K71_002331"
     CDS             complement(6087..7547)
                     /locus_tag="U2K71_002331"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316359.1"
                     /translation="MLENYIERNIFRKVYLCEQLFEFQEIDIEQTAISLRVTTPTILH
                     DLESLAECLEYCIKEQVREKHKYKLVFKHGIALSELTQFLYGQSYFLKFLSYYFNGIF
                     TSTELADLEFISLSKVYTIKKIVLDFFKENDYLKNKEIIIPEFDSRNILLALVRYVNW
                     EGYENKNPYVEEACNELIDYVETHFFKRRYSEDEKSFIIRGIEIAIGRKAFPLGLKEE
                     DKKSAETKPLFQLVCQGLEEQKGILDLQEEDVYYIFSLFNSRNYTNENMELLRKDFEV
                     VYKNFVLNDLSLNELILQIISIAEIDDAENFIFKQSFLQFVRTLWADGQVFLPERIYL
                     LSEKEQLLYGKIVEILEEWKKKNQIQLRWNENFIRKFVKSLSLINSEQAGRQEIEIFV
                     VSESSVKQFFYRTQFHLYAEEGIAEINPILFRSLEELPDECLCAKKRVVLCDAASYQN
                     GLETEKTKIYPISLKGANIHLYHIAQELHLLKKHIS"
     gene            complement(7755..8681)
                     /gene="glsA"
                     /locus_tag="U2K71_002332"
     CDS             complement(7755..8681)
                     /gene="glsA"
                     /locus_tag="U2K71_002332"
                     /EC_number="3.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutaminase A"
                     /protein_id="HEM7316360.1"
                     /translation="MEVKELEQLIGKNFHYYQTGQVADYIPALAEVDPKQLGMAIYDL
                     DTKQLIEGGDADVRFAIESMSKVPVLLLAIKDNGIEKVFQTINTEPTGFAFNSIMNME
                     INHRKHPMNPFVNAGAIATTSLIAGKDADEKFERILSFMKEICDDPNITLNEEIYHSE
                     SRTGDINRSLAYYMKGNQMIEGDVPEILDAYFRQCSLNVTATGIAKLAAVLANKGIAP
                     WNGKQIITEESATIVKSIMTTAGLYDESGEFSVHVGVPAKSGVGGGLMAAVPNRYGIG
                     VFSPALDPFGNSAAGIQLLKDVVKELDADIFE"
     gene            complement(8704..10131)
                     /locus_tag="U2K71_002333"
     CDS             complement(8704..10131)
                     /locus_tag="U2K71_002333"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7316361.1"
                     /translation="MQQSKTKIPAKKLTFFGFLAMTISMVVSLYEYPTFATSGFSLVF
                     FLLLGGLLWFIPVALCAAEMATVKGWEKGGVYTWVSRTLGKRFGFAAIFFQWFEITVG
                     YLTMLYFLTGALSYATGIGAIQNNKYLKLAILLIIFWAILLSQLKGTKYTSLIARIGF
                     VAGILLPALVLFGLGIHYVASGAPLQTILSMKTLIPDFSKLPTLVVFVSFILAYMGVE
                     TSASHANEMENPKKDYPLAMFVLVILAIILDTFGGLTVATTVPQHGLSLNTGVIQSLE
                     FLLRHIDPNLAWIAKILAILVCLGVIGEIASWVTSPSKALHVAAEEGLLPEYFAKENT
                     HGVPAHLMIANGIVATIWAAVFTLSGGGNNLSFLAAMSLTVVIYLMLYFMFFAGYLKL
                     VFKDDSLERAYQVPGGKVVKVIVACLGLLTSLFAFCISFVPPASIAAGQHGIYETILI
                     VSYLIVLVIPFTIYACRHKFQHAEK"
     gene            complement(10162..11376)
                     /locus_tag="U2K71_002334"
     CDS             complement(10162..11376)
                     /locus_tag="U2K71_002334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ammonium transporter"
                     /protein_id="HEM7316362.1"
                     /translation="MNTLPINTGDTAFMILCTAMVCLMTPGLAFFYGGLARKNNILTI
                     MSQSFLSVGITTIMWVFGGFGLAFGRDIGGVIGNPADFFLMRHVTSFPNMFHGATIPF
                     LMFFLYQLMFIVITVPLMTGAFAGRLNLKGYIILVIFWNLLIYFPVCHWIWGGGFLDQ
                     MGFRDFAGGAVIHTTAGFGSLACILTLGQRKIPLGERSQHSSLMAAAIGTGLLWFGWF
                     GFNSGGALRADEQAVNAFGSTFIALAFAMITWLIIAKVKGNGFDFVDVLTGSVAGLAA
                     ITPCAGYVEAKSAMLIGIIAGIVCHAAVDFRKKKQWDDALDVWGVHGMGGFTGTILIG
                     IFASNSHLLTNPSSWYFLGIQVIGVIITAVYAYVLTTVILKSAMHFTTITTTKEEQEE
                     GLDFFFFGNKLN"
     gene            complement(11717..11959)
                     /locus_tag="U2K71_002335"
     CDS             complement(11717..11959)
                     /locus_tag="U2K71_002335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM7316363.1"
                     /translation="MGFIWALIVGGIIGAIAGAITNKGSSMGIIANIIAGLVGSAIGQ
                     ALLGTWGPTIAGMAIIPSLIGAIILVLVVSFFARKL"
     gene            complement(11991..12548)
                     /locus_tag="U2K71_002336"
     CDS             complement(11991..12548)
                     /locus_tag="U2K71_002336"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HEM7316364.1"
                     /translation="MENKNMNTTNGSVLANEKGENRMMNGELTYEDKVIQKIIGYALK
                     DVDGLLTLDGGFFSNMAEKLVNTDNVTAGINTEVGKKQVAVDLDVIVEYGKDIEDIYN
                     QIKELISTEVNNMTHLDVIEVNVNVADIKTQEEYQKDQETVQDKVTEAAKSTGQFASR
                     QTDKAKSAVGKGAQKVKENNEPRVQ"
     gene            complement(12561..12749)
                     /locus_tag="U2K71_002337"
     CDS             complement(12561..12749)
                     /locus_tag="U2K71_002337"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2273 domain-containing protein"
                     /protein_id="HEM7316365.1"
                     /translation="MKELFSAYKLPIIFGGLGLLLAVLWLTIGFFKTLLIIIMTAIGA
                     GIGFYLKQSGIVDQFFKS"
     gene            complement(12762..13328)
                     /gene="amaP"
                     /locus_tag="U2K71_002338"
     CDS             complement(12762..13328)
                     /gene="amaP"
                     /locus_tag="U2K71_002338"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline shock response membrane anchor protein
                     AmaP"
                     /protein_id="HEM7316366.1"
                     /translation="MNKGLKIIGVIMSLLLLSVFLAIALINAPYIMPEQLERFRFFTL
                     TNYYMQQYIFWAAVVFAVLAIIILLVVLFYPKSRGTFVMKREDGKLTIDKKAIEGLVR
                     SHLHEEEFIHSPKVRIRSTKNRIHIHVNGDLKRTSSLVGKTGVLMQDIEEEVTKVLGT
                     KETVKVAVTYSGYEEQEDTRDYKHSRVE"
BASE COUNT         4065 a         2810 c         2220 g         4502 t
ORIGIN      
        1 atgatttttt gagatacttt cattgcgaat tcctctttct aaaagaacct ctcgcttgaa
       61 aaataaaaga ttgtacttta tgataattca aactgagagt ttctcagttt ctctttattg
      121 tttttcaggt cgatagagcg ccaactcttc ggcctgtatc tttgaagaaa tattcaacgt
      181 gattccgatt cctaaagaaa ggatcagata acttgttcct ccgtagctaa caaatggcaa
      241 tggcacacca gtcattggca tcagccccgc gatacttccc acgttcatta tcgtctgaac
      301 gaatagcaaa gttccgaatc ctaaaagaaa caatccagct tgctgatttt tacagcgact
      361 gcttaaacaa aagattctca tgcataatga gaacaataag aaaagcacac acaatgcacc
      421 gattagccca agctcttctg taataatcga aaaaataaaa tctgtttctg tctctggaag
      481 ataccctttt tttgtgatac tatttcctaa tccccgccca aatatgccac cattatatag
      541 tgcataaaac gaatgggtca tttgatagcc cgcaccatga ctatcgataa aagaatctct
      601 taaagtcgcg atcctttcat aagcatgatt aaacatctgt ggaagccaac ctttatttcc
      661 taaatataat accccgcctg ctgctccaat cagcaatgca gaaaaagtaa cgattagata
      721 gatccctttt ttaaatggaa ccgccgctgc ccaaaatatg acccacgcaa tcgaaaggat
      781 catcaatgct ccagcaatct ttggctgaaa taaaatcaga aaagttatac ctactgtaat
      841 caggaatggt ttttttagat cttttgggct actatttccg tcacgaaaaa accagcttaa
      901 ataaaaaatc aaaaaaagat ttgccagttc agaaggctgg aattgaatgc caaacagaga
      961 gatccaacgt tgtgcgccgt tgacagtgac accgaatatc cctattctta ctaatactaa
     1021 aaagaaaatc gataaggcta aaccgtatcc tgctatttta gggtgaagta aatagtgtag
     1081 tttgactgaa cgagctaaga agatcacgct ccaactcaaa aatatgaaaa ttagttgacg
     1141 caataataga ctttttgtat tttcatcggc ttgcaataaa cgataagaac tggcactgta
     1201 tacttctaaa agaccgatca tggacaaagt caaataagga cccaaaatcc accaatcgat
     1261 cttttttcac cgcatctcct cctcacacag caatccactt gtcaggtgag gagcgagttt
     1321 gtctcatcaa ataagtcagt cacaaaaata taaagaaaag gtcagaaacc ttttcgaaca
     1381 agaattctga cctagcatca tctcatcgtc cgagttttag cactatacaa cgtaaagaaa
     1441 acattcttag ctattggaaa aagccttaat ccaacagttt ctgttttacc cattggcgac
     1501 tttcgacgtt tctgggcaac agcctatgtt tgtataggag cctcacctaa caaggttatt
     1561 actatattct atttacgagt tcatgataga cttttcctaa tgattaggca atggaatttg
     1621 aaatgaaata gtgaaattta acaatcttta atatttttta gaaaaaaata acaaataagt
     1681 tgaaagaatc ccgttgcaaa ttctttcagt tgcaccagga ttctttgata attttttatt
     1741 tttatgtgtt tttttatatg ttttttcact tttcggaata aatatttatt tgttaaagta
     1801 attaaacaat ccgctaatcg tttgatctgg catttctatt tccttaccat ctaaagtaac
     1861 agtagtaacg acaaaatggt cttgcaatgg cggaacttct aagtactgcc cttgcagaaa
     1921 atcattcact gtttcatatt cttttttcat ttcttttgaa tctgtcgatg gtttatagct
     1981 tatttttaac ataactagtg ctccttttta ttcgggacgt gcaatcgtga aaatttctcc
     2041 tattgctgca taatcatttc ctgccaaacg gcttacagca ttcaattttc ttggatcgat
     2101 ccttcctgtc ttatcatcat agactgtttc atttaaatga tatttctgta cttctagcaa
     2161 aaataaatca gctgttactt tatttttatt ccgtatctcg acatgttcat ataaggatgt
     2221 ttcaaaacga acttttgctt ctgtcaatgc tggtactgaa atgattactg aatcagttaa
     2281 agtcaagtca gtcaggtaca gttcgctttc ctcattaggc agattggctg ctgttttatt
     2341 tacttcttct acgttgtcct catcaaggat atggataaca gcttctcttg tctcaagaat
     2401 attctttgct gtgtctttta ctgcatcttg ttttctctgg atcgccaagg ataagatggg
     2461 tcgattcgaa ctgacgatat tgaagtaact gaagggtgcg atattcacta tatcttttgc
     2521 tgatttagtc gttactactg ctactggtct agggatgact gaaccaatca gcaacttgta
     2581 attttcacgt tctgtcaatt ctgaaggact ccgttcaaac atggtttcgc cttctttcta
     2641 taaaattcgt taaaaagcag atcttgaaga caaaatgaaa agtaatgctt ttttcatctt
     2701 cttagaatct gctttaaact atctatcaat ttttcttttt tgtcaagaat ctgtgccgtg
     2761 cgtgtttttt agttatttgc tttacttgcg gcttcgacat atcttttagt gatcaattgc
     2821 ggacgggtga taatagatcc aactacgact gaaaaagcac ctaattcata tgctttctct
     2881 acttgttcag gagagtcagt atgtccttcc ataattactg ggatatcgac ttgttctatg
     2941 atttgattca ataaatcaaa atcaggtgtc tgcgtctttc gtgaatattc tgtatagcct
     3001 gctaaagtcg tagacaaaag atcaaaacca tcttcttgcg cttgaagcgc ttcctcaaaa
     3061 gtcgaaatat ctgccatagc taaccgaccg tgttcgtgaa tcatttggac aagttcagca
     3121 gttgtttttt ctgtcggacg tttacgagaa gttgcatcca gtgcaatgat ttctgcttgc
     3181 gtctccaaaa gttcttccac ttctttttct gtcgcagtaa taaacacttc tgacccatcg
     3241 taatctcttt tgataatccc tattaccggt aaatcaacga ttcgcttgat ttcattgata
     3301 tcttctgtcg tatttgctcg aataccaact gctccgcctt cttttgcagc caaagccatt
     3361 ctccccataa taaaagaact gtgtagcggc tcgttttcca aagcttgaca agaaacgacg
     3421 actccgcctt tgatttgttc cagtgtatcc acaatatctc ctccagtaat gttatataaa
     3481 aatacagctt catataaaaa tgtaaccctt ttctattcag gagacaataa tgagctattt
     3541 ctttcagcag acctttctta ctaaaagaaa attacttcaa attcttcgta tacgaccagc
     3601 ttctcttctg taaattctat gttcaacgct tctttataag catgtttaaa aaactcagca
     3661 tggactttcc gccaataagc taatgtccga tccccttctc cttctttcca agcatgctct
     3721 ttcgtgactt cactaaaagg caagatggtc acatttatat ttttgatgat acaaactggt
     3781 tcattttttc cattcaggac cacgtctaat tgaccaactt ttggtaaagg ctcattctct
     3841 tgtttataca gatcgtaacc acttgatgta ccagtcttta tcttttttac aactagttcg
     3901 ctcaactcgt ctgccatttt ttctgtatcc ccaaatgaaa agaaacctaa tttttctgat
     3961 tgataactag gatatttttc ttggaacttt ttgacatatt cttctacttg ctgatacata
     4021 acatccaccc ctttgctttt tataatgaaa aaaccatcca gatactagta taaacaatta
     4081 tctggatggt gagcataatg aattattgaa tattcaagac acgcagacta tcttcaacga
     4141 tttgttttgg tgttaaatga taacgttcat aaagctgccc tacaggaacg ctatccgtga
     4201 attctttctc tgcaccaaaa tttaatacgt tcattgaaga atcaccataa taacgactga
     4261 tattttcgcc aaatcctcct gacaaactac catcttctaa tgtgatgact aattcgtgtg
     4321 tttcttttaa tttatctaat gtttctacgt cattttctgt gatcgttcct ggattgatca
     4381 acgttgcttc gattcctttt tttctaaaag cttttactgt ttcttttcct aattcaagaa
     4441 aaccgcctaa agctaaaatt gctacttttc ctccattatt tactgtttga taggctggaa
     4501 tactataatc atttgcgata gctgtaccat gttctacacc atgtgtagga attcggattg
     4561 ctacaggctg cgctgtttgt tcgaacgccc aatctagcat agagatcata tcctcttttg
     4621 ttgctggagc aagatatttg atgttcggga tacttgataa gtaaggaatg tcaaatgttc
     4681 cttggtgtgt tcggtcacta gctgagatcg ttccgccacc gactaaaata atcactggat
     4741 tctcgttgat cgctaaatca tgtgataatt gatcatatgc ccgttgcaag aaagtacttg
     4801 attggaagac gactggacgt actccctgag aagccaaagc agaagcgaaa gtgatagagt
     4861 gctgttcagc gatccccacg tcaaaataat gatctggatg tttttcttgg aatgttttca
     4921 gtccaaaggc gcctggtatc gctgcattga ctgcggcaat aggagctgta cttgtttgca
     4981 ttttcttatc caaataatcc atgatgatca tattaaatgt ttctccagaa gttacttgct
     5041 ttgcctcccc ggtggacaga tcaaatggta catgccaatg atatttcatt ttattatcga
     5101 ttgccggctg ataaccgcga cctttttccg tatggacgtg aagtacaatc ggatgatcaa
     5161 tatcttttac ttcttcaaat aaacggatca ccgtttccag atcgtttcca ttatccacgt
     5221 aacgataatc aaatcccatt gctttaaata ggttattgct gcttttaccg ttggtttgac
     5281 gaagctcagc taagttttta tataaactgc catgattctc atcaatagac atctcattat
     5341 cgtttactaa tacgattaga ttggattgta aaacaccacc attgttcaat ccttcaaaag
     5401 ctaagccacc acttagggag ccgtcaccga taaatgctac cacatttcct gattctttct
     5461 tcagatctct cgctttagct aaacctgttg ctaagctgat agatgtagat gtgtgcccca
     5521 ctgtaaaata gtcatgttta ctttcatgtt ggttggaata tccgctcact tcacggtaat
     5581 gtcctttttc aaaaccattt tttcgaccag ttaaaagctt atgaccataa gactgatggg
     5641 aaacatccca aacgatttta tcctttggtg aatcaaatac ataatgaaac gcaatcgtta
     5701 attctactac acctagattc ggaccaacgt gcccacctac atcgctgtcc ttttgcaaga
     5761 ttttttggcg catctcttct gccaactgtt tcatttcctc aacagataat tttttgacat
     5821 ctgcggggct attgattgca tccaatacat gtaatactgt actcataata cattctctcc
     5881 tccgtactat cttcttttaa tgaaaataga tttgttacac tttatctatt ctactactta
     5941 gagttgactc taagtcaata gcattttaaa aaaatttcag agaaagcttg ttatataagt
     6001 agatcttcgc acgaatcagg actcaaataa aaaccttcta cagacaatta aaaagctttg
     6061 tagaaggtct cctaattaag gtctcatcaa gaaatatgtt tttttaatag gtgaagttcc
     6121 tgagcaatat gatacaaatg gatgtttgca ccttttaatg agattggata gatctttgtc
     6181 ttttcagttt ctaatccatt ttgatagcta gcagcatcgc acaaaaccac tctttttttc
     6241 gcacatagac attcatcagg caattcttct aatgaacgaa ataaaatagg atttatttca
     6301 gcgatgcctt cttccgcata caaatgaaat tgggttcgat aaaaaaattg tttgactgaa
     6361 ctttctgaaa caacaaaaat ttctatttcc tgtctccccg cttgttcact gttgatcaaa
     6421 gaaagacttt tgacgaattt tcgaatgaaa ttttcattcc atcttagttg gatttgattt
     6481 ttctttttcc actcttccaa tatttctaca atttttccgt acagaagttg ttctttttct
     6541 gatagaagat agatcctttc cggtaaaaat acttgtccat ctgcccacaa cgtacggaca
     6601 aattgtaaaa aggattgctt gaaaataaaa ttttctgcat cgtcgatttc agcgatacta
     6661 ataatctgta aaatcaattc attcagtgat aggtcattga gaacaaaatt tttataaaca
     6721 acttcgaaat cttttcttaa cagctccatg ttctcattgg tataatttct actgttgaac
     6781 aaagaaaaaa tataatagac atcttcttct tgtaagtcta gaattccttt ttgctcttcc
     6841 aatccttgac agactaattg aaataacggc ttcgtttctg cgcttttctt atcttcttct
     6901 ttcagtccaa ggggaaatgc ctttcttccg attgcgatct ctattcctcg tatgataaag
     6961 gacttttcat cttccgaata tcttctttta aaaaaatgag tctctacata atcaatcaac
     7021 tcgttacaag cttcttctac atacggattt ttattttcat agccttccca attgacataa
     7081 cgtactaaag ctagcaagat gttccgcgag tcaaattctg ggataatgat ttctttattt
     7141 ttcaaataat cattttcttt aaaaaaatcc aatacgattt ttttgattgt atatactttg
     7201 ctcaaagaaa taaattcaag atcagccagt tctgtactcg tgaatatacc attaaaataa
     7261 taacttaaaa actttaaaaa ataagattga ccgtacagaa actgtgtgag ctcagaaaga
     7321 gcaataccat gtttgaatac gagtttatac ttatgcttct ctcttacttg ttcttttata
     7381 caatattcta gacactcggc tagactttct agatcgtgaa ggatcgttgg tgtagtcacc
     7441 cttagagaaa tagctgtttg ttcgatgtct atttcttgga attcaaataa ttgttcacac
     7501 agatacactt ttctaaatat atttcgctcg atgtaatttt ccagcattcc tgatacacgc
     7561 ctccagtttt tcttatgatt ttatttaatc atatctgtaa atcgtatttt tgtttccttt
     7621 ttatttagaa aagccctttc aaaaaccaat aaaaaaagaa actgggacaa aagtcaaaca
     7681 agctcacaat ttcagcctca ttgtaggtag ctttgagccg tttggacttt taccccagcc
     7741 tctttcaaga atggttattc aaagatgtca gcgtctagtt ctttgactac atctttcaac
     7801 aattggatac cagctgcgct gttaccaaat ggatctagtg ccgggctgaa gacacctatg
     7861 ccgtaacgat ttggcacagc tgccatcaac ccgccgccta caccgctttt tgcaggtaca
     7921 ccaacatgga cggaaaactc tcctgattca tcatacaaac cagcagttgt cataattgat
     7981 ttgacgattg tagcactttc ttcagtaatg atttgtttgc cattccaagg agcaattcct
     8041 ttatttgcca gcacagcagc taattttgct atgcctgtag cggtgacatt gagggagcat
     8101 tgtctgaaat aagcatcgag tatctccggt acgtctcctt cgatcatttg attacctttc
     8161 atatagtaag caagagagcg attgatatca cctgttcttg attcagaatg atagatttct
     8221 tcattcaatg tgatattagg atcatcacag atctctttca taaatgacaa gattcgctca
     8281 aatttttcat ctgcatcttt accggcaatc aacgaggttg ttgcaatcgc tcctgcatta
     8341 acaaaaggat tcattggatg tttgcgatga ttgatttcca tattcatgat agaattgaag
     8401 gcaaaaccgg ttggttccgt gttgattgtt tgaaagactt tttctattcc gttgtctttg
     8461 attgctaata acaatacggg aacttttgac atgctttcga tggcaaagcg cacatcggcg
     8521 tctcctccct caatcagttg ttttgtatcc aaatcataaa tcgccatacc taattgtttg
     8581 ggatccactt cagctaatgc tggaatgtaa tccgccactt gtcctgtttg ataatagtgg
     8641 aaattttttc caatcaattg ttctaattct tttacttcca tttgaataac cagctttcat
     8701 tttttatttt tctgcatgct ggaatttatg acggcatgcg tagatcgtaa atggaataac
     8761 taagacgata agataagaaa caatcagaat cgtttcataa atcccatgct gtccagctgc
     8821 gatactagca ggtggaacaa atgagataca aaaagcaaaa agtgaagtca aaagacctaa
     8881 acaagcaacg attactttta caacttttcc gcctggaacc tgataggctc tttcaagact
     8941 gtcatcttta aaaaccagct tcaaatagcc cgcgaaaaac ataaagtaca acatcaggta
     9001 gattacaaca gtcaatgaca tcgctgcaag aaatgaaaga ttattccccc cgccgcttaa
     9061 tgtaaagacg gctgcccaga ttgttgcaac gatcccattt gcgatcatca aatgagcagg
     9121 aacaccgtgt gtgttttctt ttgcaaaata ttcaggaagt aatccttctt ctgctgctac
     9181 gtgaagtgct tttgaaggag aggttaccca agaagcaatt tctccgatga cacctaaaca
     9241 aacaagaatc gcaagaattt tcgcaatcca tgcaagattt ggatcaatgt gtcttaatag
     9301 gaattctaac gattgaatca cccctgtatt aagagacaaa ccatgttgag gtactgtagt
     9361 agcgacggtc aatccgccaa aagtatctaa aatgattgcc agaataacca atacaaacat
     9421 tgctaatgga taatcctttt tagggttttc catttcattt gcatgactgg cagatgtttc
     9481 tacgcccata tatgctaaaa taaaggaaac aaaaacaacc agcgtaggca atttcgaaaa
     9541 atcaggaatc aatgttttca tagacagtat cgtttgtagt ggagcgcctg aagcgacata
     9601 atggatccct aagccaaaca agactaatgc tggcaacaag ataccagcta caaaaccgat
     9661 ccgagcaatc aatgaagtgt atttggttcc ttttaactgt gagagtaaaa tagcccagaa
     9721 aataatcaat aggattgcca atttcagata tttattgttt tgtatagcac caatacccgt
     9781 tgcataggat aatgcaccag ttaagaaata cagcatggtc aagtagccaa ctgttatctc
     9841 aaaccattgg aaaaagattg cggcaaaacc gaatcgtttt cccaatgtgc gactgaccca
     9901 agtatagaca ccgccttttt cccatccttt caccgttgcc atctctgctg cgcacaatgc
     9961 cacagggata aaccatagca aaccgcctaa taacaagaaa aatactaagc tgaagcctga
    10021 ggtcgcgaat gtgggatatt catataatga aacgaccatt gagatggtca tagccaaaaa
    10081 gccaaagaac gttaattttt ttgcaggaat tttcgttttg ctttgttgca tgaatcttcc
    10141 tcttttctat ttgaatttgt ttcaattgag tttgttgcca aagaagaaaa agtccaatcc
    10201 ttcttcttgt tcttcttttg tagtagtgat cgttgtaaag tgcatcgccg attttaaaat
    10261 cactgttgtc agcacatagg catacacggc agtaatgatg acaccaatca cttgaatccc
    10321 taagaagtac cagctgcttg gattcgttag taaatggctg ttagatgcaa agatccctat
    10381 cagaatcgta cctgtgaatc cgcccatgcc atgaacaccc cacacatcaa gtgcatcatc
    10441 ccattgtttc tttttacgaa aatcaacagc agcatgacag acgatacccg caatgatccc
    10501 aatcagcatg gcactttttg cttctacata accggcacaa ggcgtaatgg ctgccaatcc
    10561 agcaacggat cctgttagta cgtcgacaaa atcaaagcca tttcctttta cttttgcaat
    10621 gatcagccaa gtaatcatcg caaatgcaag agcgatgaat gttgaaccaa atgcattgac
    10681 tgcttgttca tctgctcgaa gtgcaccacc ggaattaaaa ccgaaccagc caaaccaaag
    10741 cagtcctgta ccgatagcag ctgccataag actgctatgc tgacttcttt ctcctaaagg
    10801 aatctttctt tgaccaagtg tgaggataca tgctaaagag ccgaatccag ctgtcgtgtg
    10861 gatgactgct ccaccagcga aatctctaaa tcccatctga tccaaaaatc cgccacccca
    10921 tatccagtgg caaacaggga aataaatcag cagattccaa aaaataacaa gtataatata
    10981 gccttttaaa ttcaatctcc ctgcaaaagc tcctgtcatc aaaggaacgg taatcacgat
    11041 aaacatcagt tggtaaagaa agaacattaa aaatggaatc gttgcaccat gaaacatgtt
    11101 ggggaagctt gtaacatgtc tcatcaaaaa gaaatctgcc gggtttccaa taacaccgcc
    11161 gatatcccgt ccaaaggcca atccgaatcc accaaatacc cacataatcg ttgttatccc
    11221 aacggagaga aaagattgag acatgatcgt tagtatatta ttcttccgtg ctaatccgcc
    11281 gtaaaaaaaa gctagaccag gagtcatcag gcaaaccatt gctgtacata aaatcatgaa
    11341 tgctgtatct cctgtattga taggtaatgt attcattttt ttcgcctcct ttctacgatt
    11401 acgagtataa aagaaggtgt agttgttttt ttcttctgtt tttttgaatt aatacttgca
    11461 tttataaaag acagacaatt aataaaagct tgatgtgttg tgctcttttt cgtttttcac
    11521 acaaataaag acaaaaaata aacggtcttc tttttataga aaaccgttta ttttcgtctt
    11581 tttttatttt taaagatatt ttttcataag gaataaagaa tacttcatat aaaatatata
    11641 aaaaagaacc gcagcgagtg tcgctacggt tctcaaattt gttcttcatt tcaacataag
    11701 tttttcttca atttgcttat aatttacgtg caaagaatga aacgactaat actaaaatga
    11761 ttgcaccgat caatgatgga atgatagcca ttcctgcaat tgttggtccc caagttccta
    11821 gtaaagcttg tccaattgct gaacctacta aaccagcaat gatgtttgcg atgatcccca
    11881 ttgatgatcc tttattcgtg attgctcctg caattgctcc aatgataccg ccgacgatta
    11941 atgcccaaat aaatcccatt ttaaattcct cctaactgac ttcttacttc ttattgtaca
    12001 cgtggttcgt tattttcttt gactttttgt gcacctttgc ctacagcaga ttttgcttta
    12061 tctgtttggc gtgaagcaaa ttgtcctgtg gattttgctg cttctgttac tttgtcttgt
    12121 actgtttctt gatctttttg gtattcttct tgtgttttga tgtctgcaac attcacattc
    12181 acttcgataa catctaaatg tgtcatattg tttacttcag tactgatcaa ttctttgatt
    12241 tggttgtaaa tatcttcgat gtctttaccg tattctacga tcacatctag atccacagct
    12301 acttgtttct tacctacttc tgtattgata cctgctgtta cgttatctgt gttgactaat
    12361 ttttcagcca tatttgagaa aaatccgcca tctaatgtca atagaccatc tacatctttc
    12421 aatgcataac cgatgatttt ttggatgact ttgtcttcat aagtcaattc accattcatc
    12481 atgcggtttt ctcctttttc atttgccaaa actgatccat ttgttgtatt catgtttttg
    12541 ttttccatct ttgtttcctc ctatgattta aaaaattgat caactattcc tgactgtttc
    12601 aaataaaaac cgattcctgc gccaattgct gtcatgatta tgatcaacaa tgtcttgaaa
    12661 aatccaatcg ttaaccataa aacagctagc agtagtccaa gacccccaaa gataataggg
    12721 agcttgtatg ctgaaaatag ttctttcatt atttggccct cctattccac tcgagagtgc
    12781 ttgtagtctc ttgtatcttc ttgttcttca taaccgctgt atgtgactgc cactttgacc
    12841 gtttcttttg ttccgagtac tttagttact tcttcttcga tatcttgcat caatacacct
    12901 gttttcccta ctaatgaaga agttcgtttc aagtctccgt tgacatggat atggattcga
    12961 tttttcgttg aacgtatccg tactttaggt gaatgaatga actcttcttc gtgtaaatgg
    13021 gaacgtacca acccttcgat cgcttttttg tcgattgtta gtttgccatc ttcccgtttc
    13081 ataacgaaag ttcctcttga ctttggatag aaaagaacga ctagcaagat gataatagca
    13141 agaacggcaa atactacagc tgcccagaaa atatattgtt gcatgtagta attcgttaaa
    13201 gtaaagaagc gaaatcgttc caattgttca ggcataatat acggcgcatt gatcaacgca
    13261 atcgctagaa atactgaaag tagcagtaaa ctcatgataa caccgattat ttttaacccc
    13321 ttattcattt tccacctcct ataaacttat ttttaccctg taaataactc taccgacaat
    13381 cacagtatag atagcgaaca gttagcatag catcagtaaa taaagcgcta ccaaacattg
    13441 ctggatcata acggcgtttt attgccagtc tgatactgaa ataacaattg cttacttatt
    13501 ctctatttac ttttgattgc tggctatact taaaggatat tattcaagat taaaaattgc
    13561 aaagattatg ctcagttatt attacaagat tctattt
//
LOCUS       DASHHB010000066        13374 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.068, whole genome shotgun sequence.
ACCESSION   DASHHB010000066 DASHHB010000000
VERSION     DASHHB010000066.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.068"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            133..2922
                     /locus_tag="U2K71_002339"
     CDS             133..2922
                     /locus_tag="U2K71_002339"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase family protein"
                     /protein_id="HEM7316367.1"
                     /translation="MKPRTYAVVDIETTGTNPKEDRIIQFGCVMIESGRITSRFSIDI
                     HPGRKISKQIQHLTGITNQRVQKAPYFEDVAQTIYNLLADTIFVAHNIYFDYNFLNHE
                     LMRCGLPSLKIPGIDTVELAQIFLPTEPSFRLADLSESLGFIHENPHQADSDAEVTGQ
                     LLLLIEERMRKLPIITLEQIARLSRHTGMDTSRFIYHVIEEMKEKPEPLDPSLEIVDG
                     LALQKKEVELFTSVHYGERTYPRKKQAKEKMFGKTLMYRKEQNRLMNAVYDHFTKDES
                     KDLMIEAATGMGKTIGYLLPLSYLATPEKPAVISTVSLVLQQQIIEKDIPLLNQLLDQ
                     PIQPVIIKSYRHYIDLQRFKGTLVEPPEQKQYALYQMAVLVWLTQTKTGDLDELHLTN
                     LNHSFFADIAHRGIGFLARNQSFYEQDFVRFLQKKIRQSNFLIVNHAFLVQETQRKEP
                     LLPASPFLLIDEVHHLPEIAEKVNDKRLSINYFHKQIQQLEEPNQLFDRIQSYLPKEH
                     EALRWLSIYREELSALCEAQICMAEGLMSLVSGKQPNGQYPEEVMMTKEMLDQLPLES
                     GTAIDHLQLFYQEIQQLQSQMRLTFEIIQESWTNQMRIDLASLYSLFDQLDKQYLLMD
                     QWLEDWRDHLIHWFVPGNTAQQMIFHLHDFQAAMLPSTVWYPRYDRILYIGGTLKIGP
                     NRHYLSKRLGIEAAPLKVIASPYDYEKQARIYVPKEAPAVHQVSNQAYIDYLETALTG
                     LIEQEDRPILVLFTSHDVLQKVYQKMHLSMLEKGREILAQGFGGSREKLLKRFILSEK
                     SVLFGADSFWEGVDLPGDALQIVVVTRLPFDNPLRPMVKARNAYLESEGINPFYQESV
                     PKAALKLRQALGRLIRTETDRGVLLILDRRLVTAKYGARIIHALPKKVPIKELAMEEI
                     YQDIHEFLKKNEEGS"
     gene            2969..3496
                     /locus_tag="U2K71_002340"
     CDS             2969..3496
                     /locus_tag="U2K71_002340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5590 domain-containing protein"
                     /protein_id="HEM7316368.1"
                     /translation="MKIKERRPLKKKQTKEKTINRVLLGLIVFLLAVIVISSIFYLRS
                     SRPAVQAKKEAVEIAKKYANLEETDHFYWFTRKKTYFSLTGTNDKGKEIAVIIPKSGE
                     KVKVLDQTKGLTENEAKQTIAAAHPEIQIEKAALGMYDDQPVWEVVGKETNGKLNYYL
                     IGFESGDEVKTIKGI"
     gene            3517..4707
                     /locus_tag="U2K71_002341"
     CDS             3517..4707
                     /locus_tag="U2K71_002341"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM7316369.1"
                     /translation="MELSKRTKRLEPSVTLAAAAKAKALKAKGRDVLSLTVGEPDFAT
                     PENIQEAAIEAIRNGKASYYTPTAGIPELRQAIVDYLKKNYGLVYEPSQTVVTDGAKF
                     ALYTLFQTILDPQDEVIIPVPYWVSYGEQVKLAEGLPVFVKGEESNSWKVTVEQLEQA
                     RSEKTKAVIINSPSNPTGMIYSKNELQAIGEWAVKNDILIVADDIYGRLVYNGNEFTP
                     IATISEAIKNQTIIINGVSKTYAMTGWRIGYAVGNPVIINGMIAVASQSTSNPTAVSQ
                     YAAVEALKGEQDTVEEMRNAFEERLNTLYPLVAELPGVSLEKPQGAFYLFPNVKETLR
                     MCKYENVTQWVEDLLEETGVALVTGEGFGAPENVRMSYATDRATLEEAVRRIKQFIES
                     KSQN"
     gene            4747..6045
                     /gene="asnS"
                     /locus_tag="U2K71_002342"
     CDS             4747..6045
                     /gene="asnS"
                     /locus_tag="U2K71_002342"
                     /EC_number="6.1.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016180901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine--tRNA ligase"
                     /protein_id="HEM7316370.1"
                     /translation="MEQIQIIDSKNHVGETVTIGAWVANKRSSGKIAFLQLRDGTAFF
                     QGVVVKSEVSEEVFQTAKNLHQETSVLVTGEIREDSRSKFGYEIGITNVEVVSESHDY
                     PITPKEHGTDFLMDHRHLWLRSSRQHAIMQIRNEIIRATYEFFNQHHFVKIDPPILTG
                     SAPEGTTDLFETNYFDQKAYLSQSGQLYMEAAAMAFGKVFSFGPTFRAEKSKTRRHLI
                     EFWMIEPEMAFTHQEESLEVQEQYVAFLVQNVLDHCEYALDVLGRDKEVLKTYTKLPF
                     PRITYDDAIELLKKNGFDDIEWGEDFGSPHETFIANSFEQPVFILNYPKAIKPFYMKP
                     HPTREDVVICADMIAPEGYGEIIGGSERATDYDFLLEQIRNHGLDEKEYSWYLDLRKY
                     GSVPHSGFGLGLERTVTWIAGIDHVREAIPFPRLLNRIYP"
     gene            7221..7895
                     /locus_tag="U2K71_002343"
     CDS             7221..7895
                     /locus_tag="U2K71_002343"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HEM7316371.1"
                     /translation="MKKRILFIGDSITDAGRDRAEATDLGKGYPLLIAGKLISQYPTM
                     KFECMNRGIGGDKIADLQVRWQEDCLALQPDYVSILIGINDTWHNTSDQAIFGTEKSA
                     QQFESVYRKLLNELQTAEICNIILMEPFVLPITDDRHGWRKDLDPKIQIVRKLAKEYG
                     ALLVPLDGIFNALGIAYGFSKYTREDGVHPTIGGHEIIAQSWIDAIAAVDFFSGDKNE
                     RKKSEK"
     gene            7892..8233
                     /locus_tag="U2K71_002344"
     CDS             7892..8233
                     /locus_tag="U2K71_002344"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316372.1"
                     /translation="MMEIRWAVKEDLPQINQLITDHPESFVKAKTAADLNDKNKLLLV
                     AVTKEKRIIGFAQADVQQSEERVDQRPSGKLIQHTLVTKENTEEIQKKFAEYLMLEAK
                     KRRIKSIFAQF"
     gene            8263..8622
                     /locus_tag="U2K71_002345"
     CDS             8263..8622
                     /locus_tag="U2K71_002345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YccF domain-containing protein"
                     /protein_id="HEM7316373.1"
                     /translation="MSCLGNVIWFIFGGLIGGLSWLAAGILWCITIIGIPIGMQCFKI
                     ASLSFAPFGKEVIYHQSGMSLIVNIIWLIVSGLPIALAHVVSGILLCITIIGIPFAKQ
                     SFKLARLALMPFGAEIV"
     gene            9147..11231
                     /gene="cadA"
                     /locus_tag="U2K71_002346"
     CDS             9147..11231
                     /gene="cadA"
                     /locus_tag="U2K71_002346"
                     /EC_number="7.2.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cadmium-translocating P-type ATPase"
                     /protein_id="HEM7316374.1"
                     /translation="MKSYRLEGLDCANCAMKIEKGVQKINGVKEATVNFTSGKLTIDA
                     EEDHLATIEQETKKVVKELEPDVKVTEIDKEKVSEHGNEKERNTLFRILFSLAGIALL
                     LLFDFNEPIRLIGYLLIYLLIGYDVVKKAVMNIVKGKIFDENFLMSVATIGAMLIGEY
                     PEAVAVMLFYQIGEYFQGLAVSHSRKSIRELMAIRPETAHVQTTEGLMTVNPEDVQIG
                     QFVLVKPGERVPLDGTIIEGESLVDTSALTGESVPKSVYVGETVLSGFINKNKPFLVQ
                     VEKSYENSTISKLLELVENASSKKAPAENFITKFARYYTPVVVGLAVLLAVLPPLVVS
                     GAAFSEWIYRALTFLVISCPCALVISVPLSFFGGIGGASKIGVLVKGSNYLELLAQTE
                     TVVFDKTGTLTKGDFSIQTIDTKIDPKKFMQYIASAEQFSTHPIAQSVLEGYAGPLLP
                     TTNIQEFAGEGILAEVDGKQVLAGNHKLMERFEISFPLSQEIGTLLYLAIDQSYSGYL
                     VIADTLKEDAVDALVQLKQAGVKNTVMLTGDSKKIADHIGKQVGVDKIYSELLPEDKV
                     QRLEEILQRNNKKTAFVGDGINDAPVLARADVGIAMGGLGSDAAIEAADVVIMNDQPS
                     KIAEAIHLAKKTLKIVKQNIVFAIGIKILVLALGAFGFASMGDAVFADVGVTVLAVLN
                     AMRSLHVKKEDE"
     gene            11522..12988
                     /locus_tag="U2K71_002347"
     CDS             11522..12988
                     /locus_tag="U2K71_002347"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7316375.1"
                     /translation="MNNKQLRWFTVALIAFNMVWGMGNVVNNYAQQGVSVVTSWLLIL
                     AIYFIPYALIVGQLGSAFKDSKGGVSSWVENTTSNKRLAYYAAWTYWVVHIPYLAQKP
                     QAILIAGGWAVQGNGNIVNTMSVQMVAVISLIIFLAFLYLSTKGLSTLKVIGGLAGTA
                     MFVMSILFILLAVTAPFINPTMEFATPHMNQVKTYIPTFDFSYFTTVSMLVFAVGGAE
                     KISPYVNSTRNPAKEFPKGMIFLAAMVGICAVLGSVAMGMLFASDNIPKDLMANGAYS
                     AFQILGNYYGIGNSLMIIYALTNMIGQISALAFSIDAPLKILLADADPEFVPNWLRKR
                     NRKGTLVNGYLLTGILVGIIILLPIFGIKDMNELVKWLTNLNSVVMPMRYLWVFLAYM
                     MLNKAYKKFNSEYKFVKNPKIGFIFGAWCFAFTAFACILGMVPKVDYTADPKAWWFQL
                     FTNIITPIVLILLGMILPAIARRDKNKEITEPETVVQP"
BASE COUNT         4423 a         2321 c         2891 g         3739 t
ORIGIN      
        1 aaaataaacg cccagacatt tgcgtgtctg ggcgtttttg tctattttct ttaaaaagcg
       61 atgagagtat ttttctgcac tttgtagtat aatttcaaaa gaacattaga ggcaggagaa
      121 gaggaagata ttttgaaacc aagaacttat gcagtcgttg atatcgaaac gactgggacg
      181 aatccaaaag aagatcggat catccaattt ggctgcgtca tgattgaatc aggtcggatc
      241 acttcacgtt tttcgatcga tattcatcca gggcggaaga tttcaaaaca aatccagcat
      301 ttaacaggga tcacgaatca acgcgtccaa aaagcgcctt atttcgaaga tgtcgcccaa
      361 acgatttaca atcttttggc agacaccata tttgtcgcgc ataatattta ttttgattat
      421 aattttttga accatgaact catgcgatgc ggacttccta gtttgaagat accaggcatc
      481 gatacagtag aacttgcaca aattttcttg ccgacggaac caagctttcg cttagctgat
      541 ttgtcagaaa gccttggatt tattcatgag aatcctcatc aggcagatag tgatgccgaa
      601 gtgacaggtc aacttctttt gctgatcgaa gaacggatga gaaaattgcc aatcatcacg
      661 ttagagcaga ttgcgcgatt aagtagacac acaggtatgg atacgagtcg tttcatttat
      721 catgtgattg aagaaatgaa agaaaaacca gagccacttg atccctcatt agaaatcgtc
      781 gatgggctag ctttgcagaa aaaagaagtg gaactattca catccgtcca ttatggcgag
      841 cggacatatc caagaaaaaa acaagcaaaa gaaaaaatgt ttggtaaaac actgatgtat
      901 cgaaaagaac agaacagact gatgaatgcg gtctacgacc atttcacaaa agatgaatca
      961 aaagatttga tgatcgaagc agctacagga atgggtaaaa caatcggtta tctgctgcca
     1021 ttgagttatt tggctacacc tgaaaaaccc gctgtcatca gtaccgtttc gctagttttg
     1081 cagcagcaga tcatagaaaa agacattcca ttgctgaatc aattgctaga tcagcccata
     1141 caacctgtaa ttatcaaaag ctatcgccac tatattgatt tgcagcgttt taaaggcaca
     1201 cttgtggaac cgccagaaca aaaacaatat gctttgtatc aaatggcggt cttagtttgg
     1261 ctgacgcaaa cgaagactgg tgatttggat gaattacatt taacaaattt gaaccattca
     1321 ttctttgcag atatcgcgca cagaggtatt ggatttttag caagaaatca gtcattttac
     1381 gaacaagact ttgttcgttt cttgcaaaag aaaatcaggc aaagtaattt tttgattgtc
     1441 aatcatgcgt ttttagtaca agaaacacag cgaaaagaac cattgcttcc agctagtcca
     1501 tttttgttga tcgatgaagt acatcatttg ccggaaatcg ctgaaaaagt aaatgacaaa
     1561 cgattaagta ttaactattt ccataaacaa atccagcaat tagaagaacc aaatcagctg
     1621 tttgaccgaa tacaaagcta tttaccgaaa gaacatgaag ccttacgctg gttatcgatt
     1681 tatcgagaag aactttctgc gctatgcgaa gcacagattt gtatggcaga aggattaatg
     1741 agtttagtga gcggcaagca acctaacgga cagtatccag aagaagtcat gatgacaaaa
     1801 gagatgttgg atcagctgcc actggaatct gggacagcca ttgatcacct gcagcttttt
     1861 tatcaggaga tccagcagct acagtcacaa atgcgtctga cttttgagat tatccaagaa
     1921 agctggacaa accagatgcg gatcgattta gcaagtttat atagcctatt tgatcaatta
     1981 gacaagcaat acttgctgat ggatcaatgg ctggaagact ggcgagatca tttgatccat
     2041 tggttcgttc ctgggaacac agcgcagcag atgatttttc atctccatga ttttcaagcg
     2101 gctatgttgc cgtcgactgt ttggtatcct cgatatgatc gtattcttta tattggagga
     2161 acgctgaaga tcggccctaa tcgacactat ctctctaaga ggcttggcat tgaagcagca
     2221 cctcttaaag tgattgcctc tccttatgat tatgagaaac aggcaaggat ctatgtgcca
     2281 aaagaggcac ctgccgtcca tcaagtcagc aatcaagcat atatcgatta tttagagaca
     2341 gcacttacag gtctgatcga acaagaagat cggccaatac ttgtgttatt cacttctcat
     2401 gatgttttgc agaaggtcta tcagaaaatg catctgtcta tgctagaaaa aggacgagaa
     2461 atactagctc aagggtttgg tggaagccgg gagaagcttt tgaaacgctt cattttgtca
     2521 gaaaaaagcg tattgtttgg cgctgacagc ttttgggaag gtgtagattt gcctggagat
     2581 gctttgcaga tcgttgtagt cacacgttta cccttcgata atccgcttcg cccgatggtc
     2641 aaagctagga acgcttattt agaatcagag gggatcaatc ctttttacca ggaatccgta
     2701 cctaaagcag ctttgaagtt acgccaagca ttaggtcgct tgataaggac ggagacagat
     2761 cgtggcgtat tgcttatttt ggatcgaaga ctagtcactg caaaatatgg cgcacggatc
     2821 atccatgcat taccgaaaaa agtaccgatc aaagagctag ctatggagga aatctatcag
     2881 gatatccatg aattcttaaa gaaaaatgaa gaaggaagtt agaggagctg tcttttttca
     2941 taattaaaat ctgctataat gaaaaggaat gaagataaag gagcgacgac ccttgaagaa
     3001 aaaacagaca aaggaaaaaa cgattaatcg cgtgttactt ggcttgatcg tatttttatt
     3061 agcggtcatc gtgatcagct cgatttttta cttgcgttcc agtcgtccgg cggtgcaggc
     3121 gaagaaagaa gcagtagaaa tcgcaaagaa atatgcaaat cttgaggaaa ccgatcattt
     3181 ttattggttt actcgaaaga agacatactt ttcactgaca ggtacaaatg ataaaggaaa
     3241 agaaatcgcc gttatcattc ctaaatctgg tgaaaaagta aaagtattgg atcaaacaaa
     3301 gggactgact gaaaacgaag caaaacagac aattgctgcg gcccatcctg aaatccaaat
     3361 cgaaaaagct gctttaggga tgtatgatga tcagcctgtg tgggaagtag taggaaaaga
     3421 aacaaatggg aaactgaatt attacctgat tggctttgaa tctggagatg aagtcaagac
     3481 aatcaaggga atttaaaagg taaaggaatg aatgggatgg agttatcaaa aagaacaaaa
     3541 cgattagaac cgtctgtcac tttggcagct gcggcaaagg ccaaggcatt aaaagcaaaa
     3601 ggcagagatg tgctgagttt gactgtggga gaaccggatt ttgcgacacc agaaaacatc
     3661 caagaagcag caatcgaagc aatccgcaat gggaaagcta gctattatac accaacagca
     3721 ggaatcccag agcttcgaca ggcaatcgtt gattatctga agaaaaacta tggacttgtc
     3781 tatgaacctt cacaaacagt cgtaacagac ggagcgaaat ttgctttgta tacccttttc
     3841 caaacgatcc ttgacccgca agatgaagtc atcattcctg tgccttactg ggtcagttac
     3901 ggtgagcaag taaaactggc agaaggactt cctgtctttg tgaaagggga agaatccaat
     3961 agctggaaag ttactgttga acagctagaa caagcacgtt cagaaaaaac aaaagccgta
     4021 atcatcaatt ctccatcgaa tccgactgga atgatttata gcaagaatga actgcaggca
     4081 atcggtgaat gggcagtaaa aaatgatatt ttgatagttg ctgatgatat ttatggccgt
     4141 ttagtttata atggcaatga gtttactcca attgcaacga tttcagaagc aatcaaaaat
     4201 cagaccatca tcatcaacgg cgtgtctaaa acatatgcga tgacaggctg gcgcattggt
     4261 tatgcggtgg gaaatcctgt gatcatcaat ggaatgatcg ctgttgcttc ccaatcaact
     4321 agcaatccga cagcagtcag tcaatatgca gctgttgaag cattgaaagg tgagcaagat
     4381 acagtagaag aaatgcggaa cgcctttgaa gaacgcctga atacgttata tccgcttgtg
     4441 gcagaactgc cgggtgtttc tcttgaaaag ccgcaaggag ctttctatct ttttcctaat
     4501 gtaaaagaaa cgctacgcat gtgtaagtat gaaaatgtca cacaatgggt agaagatctt
     4561 ttagaagaaa caggggtagc gcttgtgact ggcgaaggct ttggggcacc agaaaatgtc
     4621 cgcatgagtt atgccactga tcgcgctacg ttagaagaag cggtacgcag aatcaagcaa
     4681 tttattgaga gtaaaagtca aaattaatcc tgaagaaatt caaggaatat aaaaggagag
     4741 tcatacgtgg aacaaattca aatcatcgat tcgaaaaacc atgtgggaga aacagtaaca
     4801 atcggcgctt gggtcgcaaa taagcgatca agcggaaaaa tcgcattttt acaattaaga
     4861 gacggaacag cctttttcca aggtgtagtt gtaaaaagcg aagtgtcgga agaagttttc
     4921 caaacagcta aaaatctaca ccaagaaaca tccgtgttag taactggaga aatccgtgaa
     4981 gacagtcgtt caaaatttgg ttatgaaatc ggaattacaa atgtggaagt ggtaagcgag
     5041 agccacgatt atccgatcac accaaaagaa cacggaacag atttcttgat ggatcatcgt
     5101 catttatggc tgcgttcttc aagacaacat gcgattatgc agatcagaaa tgaaatcatt
     5161 cgtgcaacgt atgaattctt taatcaacat cattttgtca aaatcgatcc gccgatcttg
     5221 actggttctg caccagaagg cactacggat ctattcgaaa caaattactt tgatcaaaaa
     5281 gcttatttat ctcaaagtgg ccaattatac atggaagctg ctgctatggc tttcggaaaa
     5341 gtcttttcat tcggtccgac tttccgtgct gaaaaatcaa aaacacgacg tcatttgatc
     5401 gaattctgga tgatcgaacc agaaatggct ttcactcatc aagaagaaag tttagaagta
     5461 caggaacaat atgtagcatt cttagtacaa aatgttttag atcattgcga atatgcatta
     5521 gatgtactag gacgagacaa agaagtctta aaaacttata caaaattgcc attccctcgt
     5581 attacttacg atgatgcaat cgaattactg aagaaaaatg gatttgatga catcgaatgg
     5641 ggcgaagact ttggttcacc tcatgaaacg ttcattgcga attcattcga acagcctgtc
     5701 tttatcctaa actatccaaa agccatcaag ccattctaca tgaaaccaca tccaacaaga
     5761 gaagacgtgg taatttgtgc agatatgatc gcaccagaag gttacggtga aattattggc
     5821 ggaagcgaac gggcaacaga ctatgatttc ttgctagaac aaatccgtaa tcacggtttg
     5881 gatgaaaaag aatattcatg gtatcttgat ctacgtaaat acggttctgt tccgcactct
     5941 ggttttggtc taggccttga acgaacagtc acttggattg ctggtatcga ccatgtacgt
     6001 gaagctattc cattcccacg tttgttgaac cgaatttatc cataagaggt gatgaaaaag
     6061 ctgttctgca tcaagtaatt agaacagcca agcaaaaaca gctgttcatg tttttgaatt
     6121 ggcgggactt attaccgcag atgtaagctt ttgaataccg tttagtggag aggttgtgaa
     6181 aaagctgttt agtttcgagt attaagaaaa attttgtgga aatggctttc cacatttttg
     6241 cataaatttg gcttaatacc gagaaactgg cttttgaaca ccgtttaagg aagaggttgt
     6301 gaaatcagtg ccttgacctg agtattagag aaacaaaacg gaaaaatagc ttctcatatt
     6361 ttaccgtttt tttagctaat atcgagggtc agctgattga acaccgttta tatcaagagg
     6421 ttgtgcgcct gccgttcagc tccgagtcac aaggagtaat ttcaaaaaac agtttttcat
     6481 gtttgatgac tattgtgact tgtgaccgag gagttggccg gagagcaacg tttatttaaa
     6541 aaaaagaact gcgaactctc gcagttcttt ttttaaaata cgggataaag gaagtttcca
     6601 gcagacaaga gaagaaagca aaagaaattg ctatttgatt cacaaggttc tataacgaaa
     6661 tcgcaatcaa tcaaacatat acaaagaagg ttattcaaat ggaaatctga tttgatgatg
     6721 cttctgctgt tgaaataaag cagcatctag tacctgaaaa caaactaata gtaacattcg
     6781 aagatggagt aggtccgtat tttcagcatg caatgatcca tatgtaagtt taattcatcc
     6841 ttgaaatcat cggaccagat aaagttatga ccaatctatc ccaagcaatc tagaagagat
     6901 attagtaaaa gaacatttac tggaagtctt agatgagcct ataaatatat gttctgatgg
     6961 cgtcatcagt ggtctgattg acggaagctt aggtgtgatc gatttctctg gaccaaacaa
     7021 cgaaggaatc aaggggaatc cggcacgtta agaaaaaaag tataaaaaac tagacacttg
     7081 atgagcgttc attcaattga ttgaagggac taggctggtg tcatttttta tacatatatt
     7141 ttctctttcg tcttgatagg gcttatggga taatataagg aagaaaatca tcgttttatt
     7201 caaaaaggag tttttgacaa atgaaaaaac gtatattatt tatcggtgac agcattacag
     7261 acgcaggaag agaccgagca gaagcaactg atctaggtaa aggttatcca cttttgattg
     7321 ccggaaaact aatctcccaa tatccaacga tgaaatttga atgcatgaac cgtgggatag
     7381 gaggagacaa aattgcagat ctgcaagtac gctggcaaga agattgttta gctttacaac
     7441 ctgactatgt atcgattttg atcggaatca atgatacctg gcataatact agcgatcagg
     7501 ctatattcgg aactgagaaa tctgcgcagc aatttgaatc cgtttatcga aaattactaa
     7561 atgaacttca aacagcggaa atctgcaaca tcatcttgat ggagccgttt gtacttccta
     7621 taaccgacga ccgtcatggc tggcgtaaag acttagaccc taaaatccaa attgttcgta
     7681 aacttgctaa ggaatatggc gcattgctgg ttcctttaga tggcatattc aatgctttag
     7741 ggattgctta tggtttttcg aaatatacaa gagaagatgg ggtccaccca acgattggcg
     7801 gacatgagat cattgcacaa agctggatag atgcaattgc cgcagtcgat ttttttagtg
     7861 gagataaaaa tgagcgaaag aaaagtgaga aatgatggaa atacggtggg cagtaaaaga
     7921 agatttacct caaatcaatc agctgattac tgatcatcca gaatcattcg taaaagcaaa
     7981 aacggcagct gacctaaatg ataagaataa actattgctc gttgctgtaa caaaagaaaa
     8041 acggattatc ggatttgcgc aagcggacgt gcaacagtca gaagaacggg tggatcagcg
     8101 accatcagga aaactaatcc aacacacatt agtaacaaaa gaaaatacgg aagaaatcca
     8161 aaaaaaattt gcagaatact tgatgctgga agctaaaaaa agaagaataa aatcaatttt
     8221 tgcccagttt taatcaaaaa cagtaataga aggagaaata caatgagttg tttaggaaat
     8281 gtcatctggt ttatattcgg cggactaatc ggtggtctta gctggttagc agcggggata
     8341 ctttggtgta tcacgatcat aggtatccca atcggtatgc aatgttttaa gatcgcctcc
     8401 ctttcttttg cgccttttgg caaagaagta atttatcacc agtctggcat gtcattgatc
     8461 gttaatatca tctggctgat cgtttcaggc ctaccaattg ctctcgcaca tgtcgtcagc
     8521 ggcatccttc tatgtatcac gatcataggg ataccttttg ccaagcaatc tttcaaactt
     8581 gctcgattgg cgttgatgcc attcggtgca gaaattgtat aaaaagagaa taataaaggt
     8641 tagaaaacgc atagagtcag gtgggataca tgtatttcac ctgactctat gtgttttcct
     8701 gtataagttc tcccatgtga cttttacgtc ttttatttat atgggattac tgtttatgga
     8761 aagttgaaat aattttcatt aaagaactct ttctatattt ttgaaagcgt agtggagctt
     8821 cgaatatctg ggaaggacat tttgcaggga ttgaaaaaag atattgtcct atcgaaaaat
     8881 taatgaattt agacaaattg ccagcaacag gcttcaaact tagttgctat cctgtcaaga
     8941 tcaagaaggc aagtgcgggt tggataagag caggggcaat gatcgaagag aaaaagaaag
     9001 aataagccaa aacatctgta tggaagaaat tttccgatgc agatgttttt cattttttct
     9061 ttcggatacc ttgacatatg aataaccatt catttataat tagttatgaa gagatgttca
     9121 tatgttgagg cgaggaggtc attaaaatga aaagttacag attagaaggt ttagattgtg
     9181 cgaactgtgc aatgaaaatc gaaaaaggag ttcagaagat caatggagtc aaggaagcaa
     9241 cggtaaactt cacttctgga aaactaacga ttgacgcaga ggaagatcat ttggctacga
     9301 ttgaacaaga aacaaaaaaa gtagtgaagg aattagaacc agatgtcaaa gtaacagaaa
     9361 tagataagga aaaagtatct gaacatggaa atgaaaaaga gagaaatacg ctgttccgga
     9421 ttttattttc ccttgcaggt atcgctttat tgctattatt tgattttaat gaaccaattc
     9481 gcttgatagg gtatttattg atttatctgc tgataggata tgatgtagtg aagaaggcag
     9541 tgatgaatat cgtcaaagga aaaatctttg atgagaattt tcttatgtct gttgctacga
     9601 ttggcgctat gttgatcggt gaatatccag aagccgtagc agtgatgcta ttttatcaaa
     9661 tcggcgagta tttccaagga ttggcagtca gtcattcaag aaaatcgatt cgcgaattga
     9721 tggcgatccg tccagaaaca gctcacgtgc agacgacgga aggattgatg accgtgaacc
     9781 ctgaagacgt gcagatcggt cagtttgtac ttgtgaagcc tggtgaacgt gtaccgttag
     9841 atggaacgat catcgaagga gaatcattag tcgatacgtc agcacttaca ggagaatcag
     9901 tccctaaatc tgtatatgtt ggtgaaacag tattaagcgg atttatcaat aaaaataaac
     9961 catttttagt tcaagtagaa aaatcttatg aaaattctac tatcagcaaa ttattagaat
    10021 tagtagaaaa tgccagcagt aaaaaagcac cagctgaaaa tttcattact aaattcgcgc
    10081 gctattacac accagtcgtt gtaggattgg ccgttttatt agctgtttta cctccacttg
    10141 ttgtttctgg agcggcgttt tctgaatgga tctatcgagc attgacattt ttagtgatct
    10201 catgtccatg tgcattagtg atttccgtgc cacttagttt ctttggcggt atcggaggcg
    10261 caagtaaaat cggtgtatta gtaaaaggtt caaattactt ggagttgctt gcgcaaaccg
    10321 agacagtggt attcgataaa accggcacat taacgaaagg tgacttttcg atccagacga
    10381 tcgatacaaa aatcgatcca aagaaattca tgcaatatat tgcgagtgca gaacagtttt
    10441 ctacacatcc gattgctcaa tctgttttag aaggctacgc aggtccgctt ttaccaacaa
    10501 ctaatatcca ggaatttgca ggtgaaggga tcttagctga agttgatggc aaacaagtat
    10561 tagcaggtaa tcacaaattg atggaaagat ttgaaatctc ttttccttta agtcaagaaa
    10621 tcggtacatt gctttatcta gcgatcgatc aaagttacag tggttatctt gttattgcag
    10681 ataccttgaa agaagatgcc gtagacgcat tggttcaatt aaaacaggct ggtgtgaaga
    10741 acacagtcat gttgacgggc gactcgaaaa aaattgctga tcatatcggg aagcaagtag
    10801 gcgtggacaa gatatatagc gaattgcttc ctgaagataa agtgcagcgg ttagaggaaa
    10861 tcttacaaag aaacaataaa aagaccgcat ttgtcggaga tggaatcaac gatgcaccgg
    10921 tgctagcaag agcagatgta ggaatcgcaa tgggtggatt gggaagcgat gctgcaattg
    10981 aagcagcaga tgtagtcatc atgaatgatc aaccatcgaa gattgcagaa gcaatccatt
    11041 tagcaaagaa aacattgaaa atcgtcaaac aaaacattgt ctttgctatt gggataaaaa
    11101 ttttggtact tgccttaggt gcttttggtt ttgcatcaat gggagatgca gtatttgcag
    11161 atgttggggt gacagtatta gctgttttga atgccatgcg aagcttgcat gtaaaaaaag
    11221 aggacgaatg aacgtcctct tttttttata tccttttctt aatgaaaaat gtaaagaaaa
    11281 tagataggat tataaaataa ctaatgtttt taattttgca gaaaattcaa aatagcttga
    11341 aattcaataa ggtcaatgaa ataaaagaaa ataacttatt tttttgatgg aaaaaatatg
    11401 aaaaaacgat gtgtattaaa taaaagaata cacacataaa ataacactgt cttgtaagtt
    11461 tgttaaaaat tgaatatgat tacatctgga taacaaattt ttatcataaa ggagagaacg
    11521 aatgaacaac aaacaattac gctggtttac tgttgcgtta attgcattta acatggtttg
    11581 gggcatgggg aatgtagtta ataactatgc tcagcaagga gtatcagttg tcacctcttg
    11641 gctattgatt ttggccattt attttattcc atacgcattg atcgttggac aattaggatc
    11701 agccttcaag gatagtaaag gaggcgtcag ttcatgggtc gaaaatacga catctaataa
    11761 acgactggca tactatgccg cttggactta ctgggtcgtc catattcctt atttggcaca
    11821 aaagcctcaa gcaattctga ttgctggtgg ttgggcagtc caaggaaatg gaaatatcgt
    11881 aaatacgatg tcagtacaaa tggtagcagt tatttcactt attatatttc ttgccttttt
    11941 atatttatca acaaaaggac tttctacttt gaaagtgatt ggcggactgg ccggaacagc
    12001 gatgttcgtc atgtcgatct tatttattct tttagctgta acggcaccgt tcatcaatcc
    12061 aacgatggaa tttgccacac cacatatgaa tcaagtgaaa acatatattc caacatttga
    12121 tttcagttat ttcacaaccg tttcgatgct agtatttgca gttggagggg cggaaaaaat
    12181 atccccttat gtcaactcta caagaaatcc tgcaaaggaa tttcctaaag ggatgatctt
    12241 cttagcagca atggtaggga tttgtgctgt attaggctca gtcgcaatgg gtatgctgtt
    12301 tgctagtgat aatattccta aagacttgat ggcaaatggg gcatacagtg cttttcaaat
    12361 tttaggtaat tactatggta tcggtaactc attgatgatc atttacgcat tgacaaacat
    12421 gattggacaa atctccgcat tagccttttc aattgatgca ccactaaaaa tcttgttagc
    12481 agatgcggat ccagaatttg taccaaactg gctacgtaaa cgtaatcgta aaggtacatt
    12541 ggtgaatggt tatcttctga caggtatact agtaggaatc attattttgt tgccgatttt
    12601 tggtatcaaa gatatgaatg aattagtaaa atggttgacg aacttgaact cagttgtcat
    12661 gccaatgcgt tacctatggg tgttcttggc atatatgatg ttgaataaag cttataagaa
    12721 attcaattct gaatataaat ttgttaaaaa tccaaaaatc ggttttatct ttggtgcttg
    12781 gtgctttgcc ttcactgcgt ttgcatgtat cctaggaatg gtgccaaaag tagattatac
    12841 tgctgatcct aaagcatggt ggttccaatt atttacgaat atcatcacac caatcgtctt
    12901 gatcttatta ggtatgatct tgccagcaat tgctcgtcga gacaaaaaca aagaaatcac
    12961 tgaaccagaa acagtggttc agccttaaaa tagaaatact tgttacaaga ggttgtgaca
    13021 gaagtggtca gcttcaagaa ataagaagga atttacgaaa attgcttctc aaatttttgt
    13081 gaagtttcgg cttgtttttc gaagaagcta cttctggaac accgtgaatc agaggttgtg
    13141 aaaaagctgt cctgcatcaa gtaataagaa cagccaaaca aaaatagccc ctcatatttt
    13201 tcgtttggct gggcttatta ccgcagatgc aagctttgga acaccgttta aggaagaggt
    13261 tgtaaaatca gcgatttgac ctgagtatta gagaaacaaa cggaaaaata gctcctcata
    13321 ttttgccgct ttttgagcta atgctgaagg tcagctgatt gaacaccatt tatt
//
LOCUS       DASHHB010000067        13146 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.070, whole genome shotgun sequence.
ACCESSION   DASHHB010000067 DASHHB010000000
VERSION     DASHHB010000067.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13146)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13146)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13146
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.070"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(77..149)
                     /locus_tag="U2K71_002348"
     tRNA            complement(77..149)
                     /locus_tag="U2K71_002348"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(114..116),aa:Lys,seq:ctt)
     gene            530..1099
                     /locus_tag="U2K71_002349"
     CDS             530..1099
                     /locus_tag="U2K71_002349"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1836 domain-containing protein"
                     /protein_id="HEM7316376.1"
                     /translation="MEKMRQQLMEWGKELETFRLPHWEELPDLELYMDQVRTLVDRYL
                     SPVIQGEKHPLLTSSMVNNYVKLGLIPAPVKKRYNKEHVAFLLAITTLKQVLTIPEIK
                     EGILFQGKTVGIREAYNLFCDEQEAAVWMVSQLAQGKSHPQKFDQATPVEYIAVRAAT
                     LSFAMKLLAEKTIVLETEYLKEEKTNEKQ"
     gene            1086..1946
                     /locus_tag="U2K71_002350"
     CDS             1086..1946
                     /locus_tag="U2K71_002350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HEM7316377.1"
                     /translation="MKNNKIAILVDSGTDVPEELIKKYEMYMIPLKIIYKDQVYTDKI
                     DITSEEIYQRLPEEIPGTSLPDGESITEIFEQIKKDGYEKVLAVTISSGLSGTYNVVR
                     LIAQQQEELEAYVLDTKNIGIGAGFSAIQAAKWLEEGVEWDQLISNLNELVERTKVFF
                     NVATLEYLQKGGRIGLVASIVGTALKLNPIISCNEEGIYYTVGKARGRKKSLDKTVAY
                     VKEKIGNAKVFNLAVAHGDAKEEAIEMEARLKREFPQANQIYFGQISPALVVHTGPGL
                     LGVGVQVLEG"
     gene            2136..2840
                     /locus_tag="U2K71_002351"
     CDS             2136..2840
                     /locus_tag="U2K71_002351"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016625064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7316378.1"
                     /translation="MKKVLVVDDEKPISDIVKFNLAKEGYDVYTAYDGEEALEKVAEV
                     EPDLILLDLMLPKMDGLEVAREVRKTYDMPIIMVTAKDSEIDKVLGLELGADDYVTKP
                     FSNRELVARVKANLRRGATAAKEPEEAAPAELTIGDLTIHPEAYMVTKRGETIELTHR
                     EFELLFYLAKHLGQVMTREHLLQTVWGYDYFGDVRTVDVTVRRLREKIEDNPSHPNYL
                     VTRRGVGYYLRNPEQE"
     gene            2845..4680
                     /gene="walK"
                     /locus_tag="U2K71_002352"
     CDS             2845..4680
                     /gene="walK"
                     /locus_tag="U2K71_002352"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall metabolism sensor histidine kinase
                     WalK"
                     /protein_id="HEM7316379.1"
                     /translation="MKRKVRFFRSVNFKIAITFILILLISIEIIGAYFIRGLERSTIN
                     TFIKDMNQTVESLATTISPELNRKDNADDEEVNANIKRFIENSATSDIIEIRVVDEKG
                     IIRGTTDVNEQSAVGKKNDYVDINDFTTKRYVALDNDDKRVNINVQPILSPTGDAVIG
                     ALYVKSNIEQKYSEINNTAVIFFTASLIAAFISMIVSVLVARSITQPIGEMREQAIRI
                     ARGDYSRKVKVHGQDELGQLADTFNQLAERIEEAQDTMEAERNRLDSVLSHMTDGVIA
                     TDRRGKVITINDMAVSLLDIKNEEAIGQSILTLLDIEEEYTLRKLLEDPNEMVLDRST
                     STLESDQMILRVDFSMIRRESGFISGLVAVLHDVTEQEKTERERREFVSNVSHELRTP
                     LTSMRSYIEALSEGAWKDEEVAPNFLKVTLDETDRMIRMINDLLNLSRMDTGNTQLQL
                     EYVNFNELVNFVLDRFDMMVGNQEKNYKIRREFTQRDLWVEVDTDKIIQVVDNIMNNA
                     IKYSPDGGTITCRLLETHNNVVLSITDQGLGIPKKDLNRVFERFYRVDKARARAQGGT
                     GLGLAISREVIKAHRGAIWAESKEGKGSTFYISLPYEPYEEEWWE"
     gene            4677..5993
                     /locus_tag="U2K71_002353"
     CDS             4677..5993
                     /locus_tag="U2K71_002353"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316380.1"
                     /translation="MRISEKVVRIGLILMVALSIYFSYAIWLSPAGKSSINVDETNTQ
                     MIESQSNRKTSEVFLPLHVTWHHSGMIQETNSENLVYRLQLIIDKARFGKLSEVVSGD
                     KSQFEQVKNLNSGFELAYNAPFLLREYKEAFDLDLDVSNLAKESENMYFTRIQFDQGN
                     KKIRFLNYSNHLVYEANISLNWTDINKELTAKDTKWTEMTTEPSTIDTQYATKHSIKL
                     KKYSYILSQQPYTLFRNAFFQKPDSVKNNDESNELIFYGGNETMSIHSETQVVDFRGE
                     LPEKKENASVFSESFPYISKLGSSLGNLRYFDRSDNTIDYRIFVEGFPVFGTDSRGQI
                     GVEVGGETNGSVQVNIQTSLNTIQVPIPSDEEVELASTNEIIQQLKAKGADSNKIGSV
                     IVGYTWQNVKETNRVVDLLPEWYVKYKDNWYSANDLLEKLPGLEAN"
     gene            5994..6863
                     /locus_tag="U2K71_002354"
     CDS             5994..6863
                     /locus_tag="U2K71_002354"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system regulatory protein YycI"
                     /protein_id="HEM7316381.1"
                     /translation="MDFKKIEWIFFVAFLGLNVFLFSSYHDAVYQENNVIRSNQTESI
                     QQRLASDDITYTGKISSENKQGYYLSAEQTNLSTSLANYRKAGKSGLLASGVFLDGDV
                     LTKSLSSSDAEKNVIDPDKISSSLEDFLSNKDNILFGNDYVYTKNFSHLEEEPLEIQA
                     SQEYEGIPFNDDTAKIVLSLEKNDNDYSITKYMQTHLSDIEQLREKTELYTEEEAINT
                     LYINNKIPRGSKILWRQLAYSCILKVREKNVYVPVWHVAIETSDKVVQIESVNAFSNT
                     IVTNNTVPKVEDQ"
     gene            6918..7727
                     /locus_tag="U2K71_002355"
     CDS             6918..7727
                     /locus_tag="U2K71_002355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM7316382.1"
                     /translation="MAEEKPAFKISILASGSSGNSLYIESEKKRLLVDAGLSGKKITS
                     LMAQIDRTPDQLDGILVTHEHRDHIHGVGVLARKYHLDVFANQQTWEAMEPLIGNVPV
                     EQKHLFEMGKVRTFGDIDIESFGVSHDAAAPQFYRFHKENRSFVMLTDTGYCSDYMRG
                     VIENADGYLMESNHDLEMLRMGPYPWNLKQRILGDRGHLSNEDGALVMTDVIGNRTKR
                     IYLGHLSKENNMKELAHLTMENVLKEKDFGVGHDFEIYDTDPDEATELFSI"
     gene            complement(7830..8459)
                     /locus_tag="U2K71_002356"
     CDS             complement(7830..8459)
                     /locus_tag="U2K71_002356"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HEM7316383.1"
                     /translation="MKIALIGATGHAGSFILDEALSRDLDVTAIVRHPEKLPSDVPFI
                     QKDLFELTTTDLASFDVVVDAFNAPKGKEEMHQTSLSHLIEMLEGTDTRLIVVGGASS
                     LFLDTEKTTRMIDQVPKNAPFYPTAFNMYEGLLKLKQSKNLHWTYISPASLFDPHGKR
                     TGTYLLSDDYLQRNAAGDSTISMADYAIALVDEILDEKHEKQHISVVSR"
     gene            8629..9921
                     /locus_tag="U2K71_002357"
     CDS             8629..9921
                     /locus_tag="U2K71_002357"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7316384.1"
                     /translation="MKEWLDRAFLPMITSVVNLRPLTALRNGMTYTIPIIIVGSLFFL
                     FAHFPVDYIAQWIDKTGFSMYFTQVYQATFDVIAFWIVFAIAYAYAKEEDIDRLPVGI
                     TALSAYLLLIQPLNTQIDTVWTGAEGILGAVTTGLIIGWGYARLVRLAKGWKQREKIP
                     ENVWQSFTSLIPIIVIVTFTLIGSAVFQQLFRMTVVQGVWTYVQLPIQSFIDTLFGVL
                     LLGFFVPFLWLFGLHGSAMVNGLVSPVLQANSLANAEILASGQELTVANGGHIVTQQF
                     LDQFMTVTGAGLTLGAVFFMTVFAKSKKYRELGNLALLPAFFNINESIIFSTPIVMNP
                     MMAVPFIFAPVVSGLITYSALYFGFVPLFTAVQVPWTTPPILSGFLVGGVPAAILQGI
                     VLSISFFIYFPFLKKIDSANCKKERQTKNDGAAEKMND"
     gene            complement(9997..10659)
                     /locus_tag="U2K71_002358"
     CDS             complement(9997..10659)
                     /locus_tag="U2K71_002358"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane metalloprotease"
                     /protein_id="HEM7316385.1"
                     /translation="MSLKKYSFLSIFCYGLIFISPLILASIGLVKSTSELITATAVMY
                     ILGAVVLAIFYFKQREPLAIESAVKKASAPKIVIYGLVGIFVALILQSIAVMLESVLF
                     GKATPSENTQNIIQMIMEAPAFILATTVAGPIMEEFVFRRSILGIISRYSNFWVGAVI
                     SSLLFAFAHNDGHLLIYFFLGFFFSLEYKATGRIWTSMITHVGMNTLVVLVQLAVQKG
                     LV"
     gene            10884..11168
                     /gene="groES"
                     /locus_tag="U2K71_002359"
     CDS             10884..11168
                     /gene="groES"
                     /locus_tag="U2K71_002359"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738650.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HEM7316386.1"
                     /translation="MLKPLGDRVIIEVAKEEEKTVGGIVLASAAKEKPQTGTVVAVGE
                     GRLLENGEKVPAAVKAGDQVMFEKYAGTEVKYEGKEYLIVAGKDIMAIVE"
     gene            11219..12844
                     /gene="groL"
                     /locus_tag="U2K71_002360"
     CDS             11219..12844
                     /gene="groL"
                     /locus_tag="U2K71_002360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736685.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HEM7316387.1"
                     /translation="MAKELKFAEDARAAMLRGVDKLADTVKVTLGPKGRNVVLEKSYG
                     SPLITNDGVTIAKEIELEDHFENMGAKLVSEVASKTNDIAGDGTTTATVLTQAIVREG
                     LKNVTAGANPLGIRRGIELATKAAVEELHNISTVVDSKEAIAQVAAVSSGSDKVGHLI
                     ADAMEKVGNDGVITIEESKGIETELDVVEGMQFDRGYLSQYMVTDNDKMEAVLENPYI
                     LITDKKISNIQDILPLLEQILQQSRPLLIIADDVDGEALPTLVLNKIRGTFNVVAVKA
                     PGFGDRRKAMLEDIAILTGGTVITDDLGLELKDVTIENLGNASKVVVDKDNTTIVEGS
                     GEKEAIEARVQLIKNQIAETTSDFDREKLQERLAKLAGGVAVVKVGAATETELKELKL
                     RIEDALNATRAAVEEGMVSGGGTALVNVISKVSAVEAEGDVATGIKIVVRALEEPIRQ
                     IAENAGYEGSVIVDKLKNVELGTGFNAATGEWVNMVEAGIVDPTKVTRSALQNAASVS
                     ALLLTTEAVVADKPEPAAPAAPAMDPSMMGGMM"
BASE COUNT         4369 a         2265 c         2833 g         3679 t
ORIGIN      
        1 taaaaaaaga acagcgtaaa tgttttcgaa atgctttcgc accaacattt acctgttccg
       61 tgggtttggc acccaatggg ccgtgagggg ctcgaacccg cgacccgctg attaagagtc
      121 agctgctcta ccaactgagc taacggcccg tacaaaaagt acttaataat agtagcacta
      181 agaattttaa aatgcaagac tacatttaaa gaaaatcaga aaaatcttta cttccttata
      241 ggaaatcatt catagattat tcacatttgc tcgctattat ataagcatac caacaaacaa
      301 agttcttgca agagaacaaa ccctaacact tttttcattt tttactcctt tcccgccgtc
      361 tccccggcgg gtctcctgtt cttaacgata aaagacaaga aggcttagcc ttcttgtttt
      421 ttgttttcat ggaatcccgc taatcaaatc acctagttaa agcaactaga ttgaatgttt
      481 atcataagtc tgatatcatg tttatgaaat gaaaaaagga gcggttatga tggaaaaaat
      541 gcgtcagcaa ttaatggagt gggggaagga actagaaaca ttccgtctgc ctcactggga
      601 agaactccct gacttagaac tttatatgga ccaagtcaga acgttagttg atcgttatct
      661 ttcacctgtg atacaaggag aaaagcatcc attgcttacc tcttctatgg taaacaatta
      721 cgttaaacta ggtttgatac cagcaccagt taaaaaaaga tataacaaag aacacgtagc
      781 atttttactt gcaatcacta cgttgaaaca agtattgact attcctgaaa tcaaagaagg
      841 gatattgttc caaggaaaaa cagtaggaat tcgtgaagcc tataatctat tttgcgatga
      901 acaagaagca gctgtgtgga tggtcagcca attagcacaa ggtaaatctc atccgcaaaa
      961 attcgaccag gcaacccctg tagaatatat tgctgtaaga gcggcaactt tatcgtttgc
     1021 aatgaaactt cttgcggaaa aaacgattgt tctagaaacc gaatatttaa aagaggagaa
     1081 aacaaatgaa aaacaataaa atcgcaattt tagtagattc aggtacggat gtgccagaag
     1141 aattgataaa aaaatacgag atgtatatga tcccattgaa aattatttat aaagatcagg
     1201 tatatacaga taaaattgac attacctctg aagagatcta tcaacgtttg ccagaggaaa
     1261 tccctggtac atctttgccg gatggtgagt cgatcacaga aatctttgag caaatcaaaa
     1321 aagacggtta tgaaaaggta cttgcggtaa caatttccag tggattgagc gggacatata
     1381 atgtggttcg tttaattgca caacagcaag aagaattgga agcatatgta ctagatacga
     1441 aaaatattgg tattggtgca ggtttttctg cgatccaagc agcaaaatgg ctggaagaag
     1501 gagtagaatg ggatcaactg atcagtaatc tgaatgagtt agtcgaaaga acaaaagttt
     1561 tctttaatgt tgcgacattg gaatatcttc aaaaaggtgg acggatcggt ttagttgctt
     1621 ctattgtagg aaccgcttta aagcttaacc caatcatctc atgtaatgaa gaagggattt
     1681 attacacagt aggaaaagct aggggacgta aaaaaagttt ggataaaacg gttgcttacg
     1741 tcaaagaaaa aatcggtaat gcaaaggtct tcaacttagc tgttgcccat ggggatgcga
     1801 aagaagaagc aattgaaatg gaagcgagac ttaaaagaga gttcccacaa gcaaaccaaa
     1861 tctactttgg ccagatttct ccggcattag tagtccacac tggtccggga ttattgggtg
     1921 tcggtgtaca agttttagaa ggataaatca aaaaaaggaa acactgaggt gaaaatcagt
     1981 gttttccttt tttattgaag ttagaacaaa aaataataaa gtattttgca ctttcatttt
     2041 aattttctct tttgttttat aggcaaagat gaaacaaaag cagaaatacg gtataatgag
     2101 aaggtatgac aataatatat aagaggtggc tctttatgaa aaaagtttta gttgtcgatg
     2161 atgagaagcc gatttcggat atcgtcaaat tcaatttagc caaagaaggc tatgatgttt
     2221 atactgcata tgatggggaa gaagccttgg aaaaagttgc tgaggtagaa cctgatttga
     2281 ttttacttga tttgatgcta cctaaaatgg acggcttaga agttgctcgt gaggtacgca
     2341 aaacttatga tatgccgatc attatggtaa cagccaaaga ttctgaaatt gataaagtcc
     2401 taggcttaga gttaggcgcc gatgattacg tgaccaagcc attttccaac cgagaattag
     2461 ttgcacgtgt caaagcaaat cttcgtcgcg gtgccacggc tgcgaaggaa ccagaagagg
     2521 cagcacctgc ggaattgacg attggtgatt tgacgatcca tccagaagct tacatggtga
     2581 cgaaacgagg agaaacaatc gagttaacac atcgtgaatt cgaactactc ttttacttgg
     2641 cgaagcacct tggacaagtg atgacaagag aacatttatt acaaacggtt tggggttatg
     2701 attattttgg agatgtacga acagtggatg taactgtacg tcgtttgcgt gaaaagatcg
     2761 aagataatcc aagccatcca aattatttag tgacacgtcg tggtgtcggt tactatttgc
     2821 gtaacccaga acaggagtaa ttggatgaaa agaaaagtcc gcttttttcg atcagtcaat
     2881 tttaagatcg ctattacctt tattctgatt ttattgatct cgatcgaaat tattggtgcc
     2941 tactttatac gtggtttaga acgttccacg atcaatacat ttattaaaga tatgaatcaa
     3001 acggtggaat cattagcaac aacaatcagc ccggaattga atcgaaaaga taatgcagat
     3061 gatgaagagg taaatgcgaa tatcaaaaga tttattgaaa acagtgcgac tagcgatatc
     3121 atcgaaatac gggtcgttga cgaaaaaggg atcatccgag gaacaaccga tgtcaatgaa
     3181 caaagcgctg tgggaaagaa aaatgattat gttgatatca atgatttcac gactaaacgt
     3241 tatgttgctt tagataacga cgataaacgg gtgaatatca atgttcaacc gatcctgtcc
     3301 ccgaccggcg atgccgttat cggggctctt tacgtcaaaa gtaatatcga acaaaaatat
     3361 tcggaaatca ataacacagc agtcattttc tttactgcct cactaattgc ggcatttatt
     3421 tcaatgatcg tatctgtttt agttgctcgt tcgattacac agccgatcgg tgaaatgaga
     3481 gaacaagcca ttcgaatcgc tagaggagac tacagtcgga aagtcaaagt gcatggacaa
     3541 gatgaacttg gacagctggc tgataccttc aatcagttag ctgaacgtat tgaagaagct
     3601 caagatacaa tggaagctga acgcaatcgt ttggatagcg tgctatccca tatgacagac
     3661 ggcgtcattg cgacagatcg caggggtaaa gtcattacga tcaatgatat ggctgtttcc
     3721 ttgctagata tcaaaaacga ggaagcaatc ggacaatcga ttttgacact tttagatatc
     3781 gaagaagaat atacgttgcg gaaattatta gaagatccaa acgaaatggt acttgaccgt
     3841 tcaaccagta cattggaaag tgatcagatg atcttgcgag tagacttttc tatgatccgt
     3901 cgcgaatcag gttttatcag cggattagta gctgtcttgc atgatgtgac agaacaagaa
     3961 aaaacagaac gggaacgtcg tgaattcgtc tctaatgttt cccatgaact tcgtacaccg
     4021 ttgacaagta tgcgtagtta tatcgaagca ttgagcgaag gtgcgtggaa agacgaggaa
     4081 gtagcaccaa attttttgaa ggtaacgcta gatgaaacag accgaatgat tcgaatgatc
     4141 aatgacctct taaatctatc tcgcatggat acaggtaata cccagcttca attagaatat
     4201 gtcaatttca acgagttagt gaattttgtt cttgaccggt ttgatatgat ggtgggaaat
     4261 caggaaaaaa attataagat ccgtcgtgaa tttacgcaaa gagatctttg ggtcgaagta
     4321 gatacagata aaatcatcca agtagtcgat aatatcatga ataacgcgat caagtattca
     4381 ccagatgggg gaacgattac ttgtcgctta ttagaaacgc ataataacgt ggttttaagt
     4441 atcaccgatc aaggattggg gattcctaaa aaagatctga accgagtgtt cgagcggttc
     4501 tatcgagtag ataaagcacg tgcaagagca caaggtggga caggacttgg attagctatt
     4561 tctagagaag tgattaaggc gcatcgaggc gctatttggg cagagagcaa ggaaggaaaa
     4621 gggtcgactt tttatatctc gctgccatat gaaccttatg aggaggaatg gtgggaatga
     4681 gaatctctga aaaagtcgtt cgtataggac tcattttgat ggttgcgtta agcatatact
     4741 tctcttatgc aatctggctg agcccagcag gaaaaagttc aatcaacgta gatgagacaa
     4801 acactcaaat gatcgagtct caaagcaatc gaaaaacttc cgaagtcttt cttccgcttc
     4861 atgttacatg gcaccattcg ggaatgatcc aagaaaccaa cagtgaaaat ctcgtgtacc
     4921 gattgcaatt gatcatcgat aaagcaagat ttggaaagct ttctgaagta gtttcaggag
     4981 acaaatcaca gtttgaacag gtgaaaaatc tgaactctgg ttttgagttg gcctataatg
     5041 cgcctttttt gctaagagaa tacaaggaag catttgattt agatttagat gtgtcgaact
     5101 tggcaaaaga aagcgaaaat atgtatttta ctagaataca attcgatcaa ggaaataaaa
     5161 aaatccgatt tttgaattat tcgaaccatc tcgtatatga agcaaatatt tcgttgaatt
     5221 ggacagatat caataaagaa ttaacagcaa aagacacaaa atggacagaa atgacaacag
     5281 aaccatcgac gatcgacacg caatatgcta cgaaacattc gatcaaactg aaaaagtata
     5341 gttatatcct gtcacagcaa ccctatacac tttttagaaa tgcttttttc cagaaaccag
     5401 atagcgtgaa aaataatgat gaaagcaatg aattgatttt ttacggtggg aatgaaacaa
     5461 tgtctatcca ttcggaaacg caagtagttg attttcgtgg agagctgcca gagaaaaaag
     5521 aaaatgctag tgtattttct gaaagtttcc cctatattag caaattaggc agttctttag
     5581 ggaacctcag atattttgac cgcagtgaca atacgatcga ttatcggata ttcgtggaag
     5641 gcttcccggt ttttgggaca gacagcagag gacagattgg tgtggaagtt ggcggtgaaa
     5701 cgaatggttc tgtgcaagtg aatatccaaa ccagtttgaa tacgattcaa gtgccgatac
     5761 cttcagacga agaagtagaa cttgcaagta cgaatgaaat catccagcag ttaaaagcta
     5821 agggagcaga tagcaacaag attggttcag tgatcgttgg ctatacatgg caaaatgtca
     5881 aagaaacgaa tcgtgtcgtt gatctcttac ctgaatggta tgtgaaatat aaagataact
     5941 ggtattcagc taacgacctt ctggaaaaat tacctggatt ggaggcgaat taattggatt
     6001 tcaaaaaaat cgaatggata tttttcgtcg cttttttagg gttgaatgtg tttttattca
     6061 gcagttatca tgacgcggta taccaagaaa acaatgtcat tcgctctaat cagactgaat
     6121 caatccaaca acgtctggct agcgacgata tcacttacac ggggaagatt tcgtctgaga
     6181 acaagcaagg ctattatctt agcgcggagc agaccaatct tagcacttca ttagcgaatt
     6241 atcgcaaggc tggtaaaagc ggcttgctgg caagtggtgt gtttttagac ggtgatgttt
     6301 tgacgaaatc tttatccagt tcagatgctg aaaagaacgt gatcgatcca gataaaatta
     6361 gtagttcctt ggaagatttt ttatctaaca aagacaatat tttattcggt aatgattatg
     6421 tgtacacgaa aaacttttct catttagaag aagaaccact agaaatccaa gcaagccaag
     6481 agtatgaagg catcccattc aatgatgata cagcaaaaat cgttctttct ttagaaaaaa
     6541 acgataatga ttatagcatc acgaaataca tgcagacgca tttatctgat atcgagcagc
     6601 tgcgcgaaaa gacggaactg tatacggaag aggaagcaat caatacattg tacatcaata
     6661 acaaaatacc aagaggatca aaaattttat ggcgtcagct agcttattcc tgtattctga
     6721 aagtccggga aaaaaatgtg tatgttcctg tttggcatgt agcgatcgaa acaagcgata
     6781 aagtggtcca aattgagagt gtcaacgctt tcagcaatac gatcgtgaca aacaatactg
     6841 taccaaaagt ggaagatcaa taaaaaagcg agtataatga aaaaagttta caaagtaaga
     6901 agggaagtag gagtcaaatg gctgaagaaa aaccagcttt taaaatcagt atcctagcaa
     6961 gtggaagttc gggtaattca ctttatatcg aaagtgaaaa aaaacggctt ttggtcgatg
     7021 caggacttag cggtaaaaaa atcacttcct tgatggctca aattgatcgg acaccggacc
     7081 aattagatgg aatacttgtg acgcatgaac atcgagatca tatacatggc gtgggtgtct
     7141 tggctcgtaa gtatcatttg gatgtttttg ccaatcagca gacttgggaa gcgatggaac
     7201 cactgattgg aaatgtacca gtggaacaaa aacatctatt tgagatgggg aaagtcagaa
     7261 cattcggcga tatcgatatt gagagtttcg gcgtctccca tgacgcagcg gctccgcaat
     7321 tttatcgttt ccataaagaa aatcggtcat tcgtcatgtt gacggacacc ggatattgca
     7381 gtgattatat gcgaggtgtc attgaaaatg ccgatggtta tttgatggaa agtaatcatg
     7441 atcttgagat gttacgcatg ggaccttatc cgtggaattt aaaacagcgt attttaggag
     7501 atagaggaca tctttccaac gaagacggtg cacttgtcat gactgatgtg atcggtaatc
     7561 ggaccaaacg aatctatctt ggccatctta gcaaggaaaa caatatgaaa gaacttgctc
     7621 atttaacaat ggaaaatgtt ttgaaagaaa aagattttgg cgttggacat gattttgaaa
     7681 tctatgatac agatccagat gaagcaactg agctattttc gatttaaaca gttcaatcaa
     7741 taaaaatata aaaagcagaa gataaaatca atcagggttg gttttatctt ctatttttat
     7801 ttttttagca tattaaaaaa ataaccgtat tatcgactga ctacactgat gtgctgcttt
     7861 tcatgtttct catccagtat ctcatctact aatgcaatcg cgtaatctgc catgctgatc
     7921 gtactgtcgc cagcagcgtt tctttgtaaa taatcatcac ttaacaaata agtacctgta
     7981 cgtttgccat gtggatcaaa taaagaagct ggactgatat aagtccaatg tagattttta
     8041 gattgtttca attttaacaa tccttcgtac atattgaaag ctgttggata gaagggtgca
     8101 tttttaggta cttggtcaat catacgagtc gtcttttctg tatctaagaa taacgaagag
     8161 gcacccccaa cgacaattaa gcgtgtatcg gttccttcaa gcatctcaat caaatgggac
     8221 aaagaagttt gatgcatttc ttcttttcct ttgggagcgt tgaaagcatc aacgacaaca
     8281 tcaaaagatg caagatcagt agttgtcaac tcaaataaat ccttttgtat aaacggaaca
     8341 tctgatggca atttttctgg atgacgcacg attgccgtca cgtctaaatc tctggacaat
     8401 gcttcgtcta aaataaatga acctgcatgg ccagtagctc caatgagtgc aattttcata
     8461 aaaaacctcc ttttttattc ctgtccattg taagcagttg ctgaatattc gtcaaagaat
     8521 ccgatttttg aaaagctcag tgcgcattcc tttcatggtt cgtgatcatt gggtatagtt
     8581 aaattaagaa aactgattga taaaaataga agttgaagga gataagaaat gaaagagtgg
     8641 ttagatcgtg cctttttacc aatgataaca tctgttgtga atcttagacc cttaactgct
     8701 ttgcgtaatg gaatgactta cacgatccca ataattattg ttggctcgct gtttttctta
     8761 tttgctcatt ttcctgtgga ttacatcgct cagtggatag acaaaacagg attttctatg
     8821 tattttacac aagtatacca ggctacattt gatgtgatag ccttttggat cgtttttgcc
     8881 attgcatacg cttatgcaaa agaagaagac atcgatcgct taccagtcgg cataacagcc
     8941 ttgtctgctt atctactgct tattcagcca ctaaatactc agatagatac tgtctggaca
     9001 ggtgctgaag ggatactagg agcagttaca actgggctca tcatcggatg gggctatgca
     9061 cgtttagtaa gacttgcgaa agggtggaag caaagagaga agatacctga aaatgtttgg
     9121 cagtctttta cttcattgat tccaatcatt gttatagtca cttttacgct tatcggttca
     9181 gcggtttttc aacagttgtt cagaatgact gttgttcaag gagtatggac atatgtccag
     9241 cttcccattc aatcgttcat agacacgttg tttggtgtgc ttttattagg tttttttgtt
     9301 ccgtttttat ggttatttgg tcttcatgga tctgcgatgg tcaatgggtt ggttagtccc
     9361 gtcttgcaag cgaattcatt agcgaatgca gaaatcttag cctcaggaca agagttgacg
     9421 gtggcaaatg gcggacatat cgttactcag caatttctcg atcagttcat gacggttacg
     9481 ggtgctggat tgacgttagg ggctgtattt tttatgacgg tttttgcgaa atcgaagaaa
     9541 tacagagaat taggaaatct tgctctttta ccagcctttt tcaacatcaa tgaatcaatt
     9601 attttttcca caccgatcgt gatgaacccg atgatggctg ttccttttat tttcgcaccg
     9661 gtcgtttcag gattgattac ctattccgct ttatattttg gattcgttcc tttatttaca
     9721 gcagtccaag tcccttggac aacaccgccg atcctctcgg gtttcttagt aggcggagtg
     9781 cctgcagcga ttttacaagg tatcgtgttg tcaattagtt ttttcattta tttcccattt
     9841 ttgaaaaaaa tagattctgc aaactgcaaa aaggaaagac agacaaaaaa tgatggagca
     9901 gcagaaaaga tgaacgacta aaaaacaaaa atactcctgt cgctttagat tgggaaaatc
     9961 accaatctag gatgacagga gtatttatag tcatgtttat actagccctt tttggacagc
    10021 taattgaaca agaacaacta atgtgttcat tcctacgtgt gtgatcatcg atgtccagat
    10081 ccgaccagtt gctttgtact ctaggctgaa gaagaaacct aggaaaaagt agattagcag
    10141 atgaccatca ttatgagcaa acgcaaacag caaagagctg atcactgctc cgacccaaaa
    10201 atttgaatat cgactgatga tccccagtat actgcggcgg aatacgaact cttccatgat
    10261 gggtccagca acagttgtcg ccaaaataaa agcgggtgct tccatgatca tctggatgat
    10321 attttgtgta ttctcggaag gggttgcttt gccaaacaat acagattcta acatgacagc
    10381 gatgctttgc agaatcaatg cgacaaagat tccaaccaat ccataaataa caatctttgg
    10441 agcagaagct ttttttaccg ctgattcgat cgctaagggt tcacgttgct taaaataaaa
    10501 aatagctagg acaacggcgc ctagtatata catgactgca gttgctgtga tcaattcaga
    10561 cgtagatttt acaagtccta tcgaagctag gatcaagggt gagataaaaa ttagaccata
    10621 gcaaaaaatg ctgaggaagc tatatttttt taaagacatg taattcctcc ttttaaggga
    10681 taacaagcat gcaaggcaag cttatatcga ttgatttcaa ttatatcata atttcaataa
    10741 gcagctgtca gaataaaatt tcttttcttt gatttaaaag cttgcaaaaa agagggagaa
    10801 tgagtattat taaaaatgta ggttagcact cgaaggttaa gagtgctaac agattaaaaa
    10861 aagagttatc ggagggattt atcgtgttaa aaccattagg tgatcgcgtc attatcgaag
    10921 tcgcaaaaga agaagaaaaa actgtcggag gaatcgtttt agcttctgct gctaaagaga
    10981 aaccccaaac tgggacagtt gttgcagtcg gtgaaggccg tttgttagaa aacggtgaaa
    11041 aagttcctgc cgctgtaaaa gcaggcgacc aagttatgtt cgaaaaatac gccggtacag
    11101 aagtaaaata cgaaggaaaa gaatatctga tcgtcgcagg aaaagatatc atggcgattg
    11161 ttgagtaaat ttggaagata aaaaaataat caaaaataat caatgaggtg aatcaatcat
    11221 ggcaaaagaa ttgaaatttg cagaagacgc acgcgcagct atgttacgcg gggtagataa
    11281 attagcagat acagtgaaag taacattagg acctaaaggc cgaaatgtcg tactagaaaa
    11341 atcttacggc tctccattga tcacaaatga tggtgtgacg atcgcaaaag aaatcgaatt
    11401 agaagatcat tttgaaaaca tgggtgcaaa acttgtatca gaagtagcat caaaaacaaa
    11461 tgatattgca ggagacggta caacgacagc aactgttctg acacaagcaa tcgttcgcga
    11521 aggtttaaaa aacgtaactg caggtgctaa tcctctaggc attcgtcgcg ggatcgaatt
    11581 ggcaacaaaa gcagcagtcg aagaattgca taatatctca actgttgttg attcaaaaga
    11641 agccattgca caagtagcag ctgtttcttc gggttctgat aaagttggac atttgattgc
    11701 tgatgcaatg gaaaaagttg gaaacgacgg tgtcatcaca atcgaagaat caaaagggat
    11761 cgaaacagaa ctagatgttg tagaaggtat gcagtttgat cgtggttact tatcacaata
    11821 tatggtaaca gataacgaca aaatggaagc tgttctagaa aacccatata tcttgatcac
    11881 agacaaaaaa atctctaaca tccaagatat tctgccattg ttagaacaaa tcttgcaaca
    11941 atcacgtcca ttgttgatta ttgctgacga tgtagatggt gaagcattgc caactcttgt
    12001 attgaacaaa atccgtggaa cattcaacgt agtagctgta aaagctcctg gtttcggcga
    12061 tcgtcgtaaa gcaatgcttg aagatatcgc tattttgaca ggtggtacag taattactga
    12121 cgatctaggt ttagaattga aagatgtaac aatcgaaaac ttaggaaatg cttctaaagt
    12181 tgttgtagat aaagacaata caacgatcgt agaaggttct ggagaaaaag aagcaatcga
    12241 agctcgtgtg caattgatca aaaaccaaat tgctgaaaca acttctgatt ttgaccgcga
    12301 aaaattacaa gaacgcttag ctaaattagc aggtggtgta gctgttgtta aagttggtgc
    12361 agctactgaa acagaattga aagaattaaa attacgcatt gaagatgcat tgaatgctac
    12421 acgtgccgca gtagaagaag gaatggtctc aggtggtggt acagctctag tgaacgttat
    12481 cagcaaagtt tctgctgtag aagcagaagg agacgtggca acaggtatca agatcgttgt
    12541 tcgtgcttta gaagaaccaa ttcgtcaaat cgctgaaaac gctggttatg aaggatcagt
    12601 gattgttgat aaattgaaga acgtggaact aggcactggt ttcaatgctg ctactggaga
    12661 atgggtaaac atggttgaag caggtatcgt agacccaact aaagtaacac gttctgcctt
    12721 gcaaaatgcc gcttccgttt ctgccttgtt attaacgaca gaagcagtcg ttgcagataa
    12781 accagaacca gcagcgccag cagctcctgc aatggatcca tcaatgatgg gcggtatgat
    12841 gtaagaaaaa gcaacttgct tatctacaac tgatcattag tgaaagacag ataaaaagag
    12901 gctggaacag aagcgtttaa ctccaagaaa taagaagaaa ttcacggaaa ttgcttttca
    12961 aatttttgtg aatttcagct tatttccgaa ggagttgctt ctgcttccgc tgtttatacg
    13021 tgtttagagc gtgagacaaa agtgttcttt acttttgtcc cacgctcttt tttgtgtgta
    13081 attataatga aaatagttat ttttaatagt tataatattt tatctattaa aagtaaatat
    13141 ccattc
//
LOCUS       DASHHB010000068        12992 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.071, whole genome shotgun sequence.
ACCESSION   DASHHB010000068 DASHHB010000000
VERSION     DASHHB010000068.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12992)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12992)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12992
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.071"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(94..1242)
                     /locus_tag="U2K71_002361"
     CDS             complement(94..1242)
                     /locus_tag="U2K71_002361"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cation transporter"
                     /protein_id="HEM7316388.1"
                     /translation="MAKKQEEKKKQIPQQRTKKGVLAGILGLVTNLILFVSKLMIGLF
                     SGSVSIMADAVNSLSDTASSILTLIGFKIAAKPADQEHPYGHERFEYISGLFVSIIIT
                     YVGFQFLESSAEKIIHPENVSLTPIVFFVLIISIFLKFAQGKMYQVIAQSIDSETLRA
                     TATDSYNDVFTTLAVLLSAAIEWVTGWRIDGYIGFALALYILYSGLTMLRNFVYELLG
                     SRPTPAEIEEMEEHLNACDDILGYHDLLVHNYGPNKRFASVHIEVDESMNLNVAHKII
                     DLIEKDFKKSLDVELVCHLDPMPVDNEKYYNILRQLKQILLETGTGLNIHDFRVIHQG
                     KTLQFDVVVPEKFQFVDTELERILRSQIEEKIGDYQLEITFDHNYLLY"
     gene            complement(1364..1960)
                     /locus_tag="U2K71_002362"
     CDS             complement(1364..1960)
                     /locus_tag="U2K71_002362"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HEM7316389.1"
                     /translation="MNSRNKTFRLVLRAILLAIIIVQAMVPWLGFIPLGFISLTIIHI
                     TVIIAAVVLGPKDGMVIGLFWGIATIVRAYAMPTTPFDTLVFTNPIISVVPRVLVGLV
                     AGLVFHWIYQKNKSITISSAFAGVLGSLVNTVLVLGFMGLFYTGATADAYGVDSALLF
                     KTLAGVAAINGIPEAIGAGIITPLLAKALFAATPLKPE"
     gene            complement(2160..4802)
                     /gene="alaS"
                     /locus_tag="U2K71_002363"
     CDS             complement(2160..4802)
                     /gene="alaS"
                     /locus_tag="U2K71_002363"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HEM7316390.1"
                     /translation="MKQLSSAQVRQMFLDFFQSKGHTIEPSASLIPVNDPTLLWINSG
                     VATLKKYFDGSVVPENPRITNAQKSIRTNDIENVGKTARHHTMFEMLGNFSIGDYFKN
                     EAIHWAWEFLTDPKWMAFDPEKLYVTVYPKDTEAKRIWHEEVGLPLDHIIDVEDNFWD
                     IGAGPCGPDTEIFYDRGEAYNDLAEDDPENYPGGENERYLEIWNLVFSEFNHKPDNTF
                     EPLPHKNIDTGMGLERMVSIVQDASTNFETDLFMPIIHAVEKLSKQVKYGENPVTDIS
                     YKVIADHIRALSFAIGDGALPSNEGRGYVLRRLLRRAVMHGKKLGIEQAFLYRLVPVV
                     GEIMVSYYPEVLQQKEFIEKVIRTEEERFNETINEGLEILNQVIQEVKNENGTTLAGK
                     DIFKLYDTYGFPVELTEEVAEDEGLKVDHAGFEKEMDAQRERARSARSTEKSMGVQSA
                     LLTDIKVESKFIGYTEQESDSQLLVIVKGESLLNEVAEGEAQMIFDQTPFYAEMGGQV
                     ADQGWIMNEVGEIVAVVTDVQKAPNGQFMHTVEVKAPMSEGQTYHLLIDQSMRNRIIK
                     NHTATHLLHKALKEVLGEHANQAGSLVAPGHLRFDFTHFGQITSDELAEMERIVNEKI
                     WEAIPVETIETDIDTAKNMGAMALFGEKYGHDVRVVNIGGWSIELCGGTHVANTENIG
                     IFKIVSESGIGAGVRRIEAVTSKEAYELLEEEEKQLKQVATIVKSPQLKEVVSKAEQL
                     QQQVRELQKENEQLASKLANQQAGDVFKDPETINGKTIIAAQVKVKDMNQLRQLADQW
                     KQKNISDILVLANEQEGKVSLLAAMTKESNEAGLKAGDLIKAIAPKVGGGGGGRPDMA
                     QAGGKNPAGIPDALKAAKEWVAQQ"
     gene            5190..5621
                     /locus_tag="U2K71_002364"
     CDS             5190..5621
                     /locus_tag="U2K71_002364"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7316391.1"
                     /translation="MKKIVGADEWIRAASCFLRYQVFVLEQGIPAELEFDGKDIPQQT
                     YYLFLDQKRPVATLRYEKKTPTCFKPERFCVAKEYRGKGLGGQLLEAAEKRAVSEGCQ
                     TSYVVAEMTAVDFYEKQGYHTCTEPFIEDEVLCIGMKKSLA"
     gene            complement(5666..7015)
                     /locus_tag="U2K71_002365"
     CDS             complement(5666..7015)
                     /locus_tag="U2K71_002365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HEM7316392.1"
                     /translation="MNNFDIFRFKPFINEALTAKGFKQPTEVQERLIPLIAKGKNIIG
                     QSQTGTGKTHTFLLPLFEKINAEKEEVQVVITAPSRELAAQIYEAATQIAKFSTPEIR
                     VSNFVGGTDKQRQLNRLHHQQPQVVVGTPGRILDMMNEQALKIHTAFAFVVDEADMTL
                     DMGFLNEVDQIASRLPEKLQFLVFSATIPEKLRPFLRKYMENPIIEEIKPKAVISEDI
                     ENWLISTKGKDENQMIYQLLTIGHPYLAIVFANTKQHVDEIADYLKNQGLKVAKIHGD
                     ITPRERKRVMRQVQNLEYQYVVATDLAARGIDIEGVSHVINAEIPEDLDFFIHRVGRT
                     GRNGLKGTAITLYSPNDEQAIDAIEKLGVSFEPKEIKNGEIVKTYDRNRRAKREKSKD
                     VLDPTLIGLVKKKKKKIKPGYKKKIDWAIAEKNKKERKIERRQQSRTARKNKKNSHK"
     gene            complement(7085..8248)
                     /locus_tag="U2K71_002366"
     CDS             complement(7085..8248)
                     /locus_tag="U2K71_002366"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HEM7316393.1"
                     /translation="MASYIEPIRFALIVFPFLALAISSVFFIYEYRKYGTFLVTRAVI
                     LYSFVFYLLCAYFLVILPLPAKETVAQMTGPKMELHLFASWQHFRDNTVLVLSEPSTY
                     LPAMKQSVFLEPAFNVLLLIPFGIYLRYYFRLSFMKTMIASFCLSLFFELTQLSGLYF
                     IYPRPYRLFDVNDLVTNTLGGIIGFVVTPLFTFMLPTRRRMDELSYEKGKTVSLTRRL
                     VAYLIDWTFLSLINLVAAIFFKIAFDSGAVTSHIWWLLLEVGVYFMIVPFITNGWTLG
                     KKIVRIRLVEEDRERISLKALMIRYGYLYFLFYGLSWLGQLLQQIMNSTTNTVLQMSA
                     VFISLGVLLLQGIFILNILLSFLRKKRILFYEKASHTYTVSTIGQTKDDDSRN"
     gene            8377..8700
                     /locus_tag="U2K71_002367"
     CDS             8377..8700
                     /locus_tag="U2K71_002367"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316394.1"
                     /translation="MLNMYFVFGVPIFLLFLYATIAYVRKRTTIHYLGFILLIISGFM
                     LVFNLQTWQQALLEMDKMTPHALSKVLGYPVYLIWLPIFISGCLVLLNIYRGVRRIVQ
                     LRKSK"
     gene            complement(8883..10232)
                     /locus_tag="U2K71_002368"
     CDS             complement(8883..10232)
                     /locus_tag="U2K71_002368"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002315079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7316395.1"
                     /translation="MEEYQATPVKEVKIENELKRTMGFFTALSTVMGTVIGAGVFFKA
                     ASVAEVTGSASLHMFSWFLGGMISVCAGLTGAELAAAIPETGGMIKYIERIYGNTAAF
                     LLGWAQVVIYFPANVAALSIIFGTQFVNLFGLSQSMIVPVAVTAAVSILLINFLGSKA
                     GGAFQSITLVCKLIPLFVIVIFGLFRQEGVDFQLFPIQAGENLSFFSALGAGLLATMF
                     AYDGWIHVGNISGELKKPAKDLPKAISLGIIGIMIVYLLVNAVFLRTASIDGVAGNSN
                     AASDVAKMIFGGFGGRLVTVGILISVYGTINGYTLTGMRLPYVMAKENNLPFSKLFAK
                     LHDKTKVPVAAGILELVIAIGMMMIGGFDTLTDMLIFVIWIFYTMVFVGVILLRKKEP
                     DLFRPYKVPMYPFIPLVAIIGGTFIVSSTLITQTFLASMGIVLTLAGIPIYLYLKRR"
     gene            complement(10301..11254)
                     /locus_tag="U2K71_002369"
     CDS             complement(10301..11254)
                     /locus_tag="U2K71_002369"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonine 3-dehydrogenase"
                     /protein_id="HEM7316396.1"
                     /translation="MKKVLVTGCLGQIGSELITRLRQELGSENVIATDIRMPEVIEKN
                     DIFEILDVMDYEKMRSIAEQYQVDTLIHLAALLSAVAEAKPQLAWNLNVTGLVNALEV
                     AREFQLKFFTPSSIGAFGPNTPKDHTPQDTLQRPTTMYGVTKVAGELLCDYYYVKYGV
                     DTRGVRFPGLISYATLPGGGTTDYAVDIYYSAIKEGKYTSFIDQGTSMDMMYMPDAID
                     AIIKLMNADPDRLVHRNAFNISAMSFDPEEIKASIQKIIPDFEMDYDVDPVRQSIAES
                     WPNSLDVSCAQEEWGFSPKYDLDVMTKDMLEKLKEKLVETV"
     gene            complement(11356..12546)
                     /locus_tag="U2K71_002370"
     CDS             complement(11356..12546)
                     /locus_tag="U2K71_002370"
                     /EC_number="2.3.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine C-acetyltransferase"
                     /protein_id="HEM7316397.1"
                     /translation="MSSKELTMYLEKELDQLKEKGLYNTIDILESENGACIIVDGKKM
                     INLASNNYLGFANREELKKACIEATETYGVGAGAVRTINGSLKIHQQLEEKIAEFKGT
                     EAAIAFQSGFNCNMGAISAVMTKEDAILSDELNHASIIDGCRLSGAKIIRIKHQDMKD
                     LEKKAKEAIESKKYKKIMYITDGVFSMDGDIARLPEIIPIVEKYGLITYVDDAHGSGV
                     TGKGAGTVKHFGLSDKIDMQMGTLSKAIGVVGGYVAGSKTLIDWLKARSRPFLFSTSL
                     TPGAAAAALASITLMQEHPELVEKVWENANYFKEELKKVGYNIGMSETPITPVILGDE
                     KVTQTFSKKLIEHGIYAKPIVYPTVPLGTGRIRNMPTAEHTREMLDEAVAVYQKIGKE
                     MEII"
BASE COUNT         3747 a         2729 c         2237 g         4279 t
ORIGIN      
        1 ataatcttgc acgtcaggct ctccataaac aattaaaaga aaaaaagact ctttaaatta
       61 aaaactttaa ggagtctttt tatagtttct catttaatat aaaagataat tgtgatcaaa
      121 cgtaatttcc aattggtaat ctccgatttt ttcttctatt tgactacgta agattctttc
      181 tagttctgta tcaacaaact ggaatttctc agggaccact acatcaaatt gcagcgtttt
      241 tccttgatga attactctaa aatcatgaat attaagccct gtacctgttt caagaagaat
      301 ttgctttaac tgccgcaaga tattatagta tttttcgtta tcaacaggca ttggatccaa
      361 gtgacagaca agttccacat ccaaagactt tttaaagtct ttttctatca gatcaataat
      421 cttatgagcc acattcaaat tcattgattc atctacttcg atgtggactg atgcaaagcg
      481 tttattgggt ccatagttat gcaccaataa atcatgatag ccaagtatat catcgcaagc
      541 atttaaatgc tcttccattt cttcaatctc tgctggcgtt gggcgacttc ctaatagttc
      601 gtatacaaaa tttcgaagca tcgtcaatcc actataaagg atatataatg ctaaagcaaa
      661 acctatatat ccatcaatac gccatccagt tacccattcg attgctgctg aaagcaatac
      721 agccagtgta gtaaagacat cattataact atctgttgct gtagctctca atgtttcaga
      781 atcaatggat tgcgcaataa cctgatacat tttgccttgt gcaaatttca aaaagatgga
      841 aatgattagg acaaaaaaga cgatgggtgt taaagagaca ttttctggat ggatgatttt
      901 ttcagcagaa gactctaaaa attgaaatcc tacataagta atgatgatcg aaacaaataa
      961 cccactgata tattcgaagc gttcgtgtcc ataaggatgt tcctgatctg ctggctttgc
     1021 agcaatttta aatccgatca aagtcaatat ggatgaagca gtatctgata gactgttcac
     1081 tgcatcagcc ataatggaga cacttcctga aaaaagcccg atcattagct tagagacaaa
     1141 caagatcaga ttcgtaacca atcctaagat acccgctagt actccttttt ttgtccgctg
     1201 ttgcggtatt tgtttctttt tttcttcttg tttcttagcc aagtttttta ccccttctag
     1261 tttgcttgtt gctcaggtaa aaagatggag aggacgattg ttttcctctc ctctcttcct
     1321 ttttctatct atcgtgcgtc ctattactat taaaagatgg tcattattct ggtttcaacg
     1381 gtgttgctgc aaataacgct tttgctagta atggtgttat gattcccgca cctattgctt
     1441 ctggtatccc attgattgct gcaacccctg ctaaggtttt aaataaaaga gcagagtcta
     1501 caccataggc atctgccgtt gcacctgtat aaaataatcc catgaatcct aacactaaga
     1561 ctgtattgac caatgaaccc agtacccctg caaaggctga gctgatcgtt attgatttat
     1621 ttttttgata aatccagtgg aaaaccaagc ctgctactaa gccaacaagt actctgggaa
     1681 ctaccgagat aatcggattc gtgaacacta atgtatcaaa tggcgttgtt ggcatggcat
     1741 aggcacgaac aattgtagcg attccccaaa acaagccaat caccatgcca tcttttgggc
     1801 ctaatactac tgcagcaata attactgtga tatggataat tgttaaacta atgaatccta
     1861 atggaataaa ccccagccaa ggaaccatcg cttgtacgat gatgattgct aacaagatcg
     1921 cacgtaagac tagtcgaaag gtcttatttc tactattcat tttttcaccc ctttttattt
     1981 atactaatcg taaaaatttc tgtcactttc gtcaactaaa gaccatccga atccgaccat
     2041 ttgattagat gcgccatcca attgaaaaaa gtatgagggc aagacttgtt tgtcttcgcc
     2101 cccataatca tgtttcgttc aatgtttgaa cttttgtttt tctgtcaaac gtaaacgccc
     2161 tattgttgcg caacccattc tttcgcagct ttaagtgcat ctggaatacc agcaggattt
     2221 ttcccgccag cttgtgccat gtctggacgt ccaccaccgc caccgcctac tttcggtgcg
     2281 attgctttaa tcaagtcgcc agcttttagt ccagcttcat ttgattcttt cgtcattgcc
     2341 gctagcagac tgacttttcc ttcttgttcg tttgctaaaa caagaatatc tgaaatattt
     2401 ttttgtttcc attggtcagc taattgacgc agctgattca tatctttcac ttttacttgt
     2461 gctgcaatga ttgtttttcc attgatcgtt tctggatctt tgaatacatc accagcttgt
     2521 tgattcgcta acttgctagc taattgttca ttttcctttt gtagctctct tacttgctgt
     2581 tgcaattgtt ccgcttttga aacaacttct ttcaattgag gtgatttaac aattgttgct
     2641 acttgtttta gttgtttttc ttcttcttca agtagttcgt acgcttcttt acttgttaca
     2701 gcctcgattc gtcgcacgcc tgcaccaatt cctgattcag aaacgatttt gaagattccg
     2761 atgttttctg tatttgctac atgtgtgcct ccgcacagtt cgattgacca gccgccaata
     2821 tttactactc gaacgtcatg tccgtatttt tcaccaaaca aagccattgc acccatgttt
     2881 ttggctgtat cgatatctgt ttcaattgtt tcaactggga tagcttccca gattttttcg
     2941 ttgacgattc tttccatttc agctaattca tctgaagtga tttgaccaaa gtgggtaaag
     3001 tcgaatcgta aatgtccagg agcgactaaa gaaccagctt gatttgcatg ctcgccaagt
     3061 acttctttca atgccttgtg cagtagatgg gttgctgtat ggtttttgat gatccgattg
     3121 cgcatcgatt gatcgatcaa tagatgatag gtttgtcctt cactcattgg tgcttttact
     3181 tctacggtgt gcataaattg accattaggc gctttttgaa catctgtaac aactgctacg
     3241 atttcgccta cttcattcat gatccatcct tgatccgcta cttgcccacc catttctgca
     3301 tagaaaggtg tttgatcaaa gatcatttga gcttcacctt ctgcgacttc attcaaaaga
     3361 gactcacctt taacgatgac caataattga ctatctgatt cttgttctgt gtatccaatg
     3421 aatttacttt ctactttgat atctgtcaat aaagcagatt gtacccccat tgatttttca
     3481 gtactgcggg cagagcgagc acgttcgcgt tgtgcatcca tttctttttc aaaaccagca
     3541 tgatcaactt tcagtccttc atcttctgcc acctcttccg ttaattctac cgggaagcca
     3601 tatgtgtcgt atagtttgaa gatgtctttt cctgctagag tcgtcccgtt ttcgtttttt
     3661 acttcttgga tcacttgatt taagatttcc aaaccttcat tgattgtttc attgaaacgt
     3721 tcttcttctg tacggataac tttttcaata aattcttttt gttgaagtac ttcaggataa
     3781 taacttacca tgatttcccc aacaacaggg actaaacgat ataagaatgc ttgttcaata
     3841 cctaattttt tcccatgcat gacagcacga cgaagcaaac ggcgtaagac ataccctctc
     3901 ccttcattcg atggcaatgc accgtcgcca atagcaaatg aaagggcgcg aatatgatct
     3961 gcgattactt tgtaagaaat atctgttact gggttttcac catattttac ttgtttgctt
     4021 agcttttcta cggcgtggat aataggcata aacaagtctg tctcaaagtt cgttgacgca
     4081 tcttgaacga ttgagaccat gcgttccaat cccatccccg tatcaatgtt tttatgagga
     4141 agtggttcaa atgtgttatc aggcttatgg ttgaattctg aaaatactaa gttccagatt
     4201 tctaaataac gttcattttc tcctcctgga tagttttcag gatcgtcttc tgctaaatcg
     4261 ttataagctt ctccacggtc atagaaaatt tccgtatccg gaccacatgg gcctgcaccg
     4321 atatcccaga agttgtcttc tacatcaata atgtgatcta gtggcaggcc aacttcttca
     4381 tgccagatgc gttttgcttc tgtatctttt ggataaactg ttacataaag tttttctgga
     4441 tcgaatgcca tccattttgg atcagtcaaa aattcccatg cccagtggat ggcttcattt
     4501 ttgaaatagt caccaatcga aaaattaccc agcatctcaa acatcgtatg gtgccgtgct
     4561 gttttaccga cattttcgat atcatttgtt cgaatagatt tttgggcatt tgtgattctt
     4621 ggattttctg gcacaactga accatcaaaa tattttttca atgttgcaac accagagttg
     4681 atccacaata aggttggatc gttcacgggg atcaaagacg cactaggttc aatcgtatgt
     4741 cctttagatt ggaagaaatc aagaaacatt tgtcgaactt gtgcactact taattgtttc
     4801 atgtcattac ctgctttcta taaattaaat acaaaaaaac accatcacag ccaaggacga
     4861 aaactcgcgg taccaccttg attgcaatga tgtgtaatca ttacctctta aactcgataa
     4921 cgctgagctg cgtcggtatt tctaccaaaa tagaaaaagg gagcaatttg attagtgact
     4981 attctttttt cagccaagaa agaattttct gtgagtctta aaaaacaaat catatccttt
     5041 tcgtacttaa ttagtatacg cattctattg atcactgtca atatctcaat ttttattttc
     5101 atttcctttt ggtcatactc cacgattttt ggtacgataa gaataaagga atcaatacat
     5161 ctctgctatt catgacaagg aggaatatga tgaaaaaaat agttggagcg gatgaatgga
     5221 tacgcgcagc tagctgcttt ttgagatatc aagtttttgt attagaacaa gggattcctg
     5281 cagaactaga attcgacgga aaagatatcc cgcaacaaac ctattatcta tttcttgatc
     5341 aaaaaagacc agtagctact ttgcgttatg aaaagaaaac acctacttgc ttcaagccgg
     5401 aacgattttg cgtagcaaaa gaataccgag gaaaagggtt aggcggtcag ttgcttgaag
     5461 ctgctgaaaa acgggcggtt tcagaaggtt gtcagactag ttatgtagta gctgaaatga
     5521 cggctgtaga tttttatgaa aaacaagggt atcatacttg cacagaacct tttattgaag
     5581 atgaagtact ttgtatcgga atgaaaaaaa gtctagctta aaaacaagag attgtgacac
     5641 gccttttgtc acaacctctt gttttttatt tatgtgaatt tttcttattt ttacgagcag
     5701 tacggctttg ctgtctgcgt tcgattttgc gttctttttt atttttttct gcaattgccc
     5761 aatcgatttt tttcttataa ccaggcttga tctttttctt tttcttcttc acaagaccaa
     5821 tcagcgtggg atctaataca tcttttgatt tttctcgttt ggcacgtcta ttacgatcgt
     5881 atgttttgac gatttccccg tttttgattt cttttggttc aaatgagacg cccaattttt
     5941 ctatcgcatc aatggcttgc tcatcatttg gcgaatataa cgtaatggcc gttcctttta
     6001 agccattacg tccagtacgt ccgacacgat ggataaagaa gtctagatct tctggaattt
     6061 ctgcattaat aacatgagag acaccttcga tatcgatccc tctagcagct aaatcagtcg
     6121 ctacaacata ttgatactcc agattctgta cttgtcgcat cactcgtttt ctctctcttg
     6181 gggtgatgtc tccatgaatc tttgctactt tcaagccttg gtttttcaag taatcggcaa
     6241 tttcatccac atgctgtttt gtattggcaa atacgattgc taaataagga tgaccaattg
     6301 tcagcagttg ataaatcatt tgattctcat ctttaccttt tgttgaaatc agccaatttt
     6361 caatgtcttc agaaataact gcttttggtt tgatttcttc aatgattgga ttttccatgt
     6421 attttcgtaa gaatggacga agtttttctg gaatagtggc agaaaaaact aaaaactgca
     6481 gcttttctgg taatcgactt gcaatttggt ctacttcatt taaaaaaccc atatccaatg
     6541 tcatatccgc ttcatcgaca acaaatgcaa atgcggtgtg aatcttcagc gcttgttcat
     6601 tcatcatgtc taatattcta ccaggcgtgc ctactacaac ctgtggctgt tggtgatgca
     6661 gacgatttaa ttgacgctgt ttgtctgtac cgccgacaaa attcgagaca cggatctccg
     6721 gtgtagaaaa tttagcgatt tgtgtagctg cttcatagat ctgtgcagct agttccctac
     6781 ttggagctgt aataacaact tgtacttcct ctttttctgc attgattttt tcaaacaaag
     6841 gcaacaaaaa cgtatgtgtt tttcctgtac cagtttgaga ttgcccaatg atattttttc
     6901 cttttgcaat taaaggaatc aaacgttcct ggacctctgt gggctgtttg aagccctttg
     6961 cggttaacgc ttcattgata aacggtttga agcggaatat atcaaaatta ttcattcgtg
     7021 cacctcttct ctttaaatta tcacgacttt ggcattataa cacagacaaa gcgtttttgc
     7081 tcagctaatt tcgagaatca tcatcctttg tttggccaat cgtgctaaca gtatatgtgt
     7141 gactagcttt ttcataaaac agaatccgtt tttttctcaa aaatgatagc aagatattta
     7201 aaataaaaat accttgtagt aacaagacac cgagagaaat gaatactgca ctcatctgca
     7261 acaccgtatt cgttgtcgaa ttcatgattt gctgaagcaa ctgccccaac cagcttaaac
     7321 cataaaaaag aaagtacaga tacccgtagc ggatcatcaa tgcttttaaa gaaattcgtt
     7381 cgcggtcttc ttccaccaaa cggatacgca caattttttt gccaagtgtc catccattgg
     7441 ttataaacgg tacaatcata aagtataccc ctacttctaa taaaagccac cagatatgag
     7501 aagtaactgc ccctgaatcg aacgcaattt taaagaaaat cgccgctacc aaattaataa
     7561 gagataaaaa tgtccagtcg atcaaataag cgaccaagcg tcgagtcaat gagacagtct
     7621 ttcctttttc ataagaaagt tcatccattc gtcttcttgt aggaagcata aatgtaaata
     7681 acggcgtaac gacaaaacca atgattccac ccaatgtatt tgtcactaaa tcattcacat
     7741 caaataagcg gtatggtctt gggtagataa aataaagtcc acttagctgc gtcaattcaa
     7801 aaaatagaga aaggcagaaa ctagcaatca tcgttttcat aaaagaaagc cggaaataat
     7861 atcttaaata aatgccaaac ggtatgagta ataaaacatt aaaagctggt tccaaaaata
     7921 cactctgctt cattgccggc aggtaggtac taggttcaga caagaccaat accgtgttgt
     7981 ctctgaaatg ttgccatgaa gcaaataaat gcagctccat ctttggccct gtcatttggg
     8041 caaccgtctc ctttgccgga agtggtaaga tcaccaaaaa atacgcacac aataaataaa
     8101 aaacgaaaga atacaaaata actgcccttg ttactagaaa tgttccatac tttcgatatt
     8161 cataaataaa aaaaacagat gagatcgcta aggctaaaaa tggaaaaaca atcagcgcaa
     8221 aacgaatcgg ttcgatataa cttgccatat gactcctcct tgattctttg tatctctctt
     8281 atcttagcag tcctttgctg tttcaagcaa gtaacgaggc tattatttct catttctttc
     8341 aaagtaaagt aagataaaca caaagggggt gtcgagatgc tgaatatgta ctttgttttt
     8401 ggcgtaccta tttttttact gttcctttat gctacgatcg cttatgtaag aaaaagaact
     8461 acaattcatt atttaggatt tattctcctg attatttcgg gtttcatgct agtcttcaat
     8521 cttcagactt ggcagcaagc acttcttgaa atggataaaa tgactccaca tgctttgtct
     8581 aaagtgcttg gctaccctgt ctatctgatt tggcttccca ttttcatttc tggttgcttg
     8641 gttttattaa atatttatcg cggcgtacgc cgaatagttc agttacgcaa atcaaagtaa
     8701 aagacagaaa tcggttgagc ccgatttctg tcttttactt tgctatttca tcgtagacaa
     8761 aacgacattt acgtgatcaa aattaacgag tacttttgac atttcttcca cgtactcctc
     8821 atttaaataa aatattttgt ttggcagaga agtgcagttc taataaaatt cgaatgtttt
     8881 agctatctac gttttaaata gagataaatc gggataccag caagtgtcaa tactatcccc
     8941 atcgaagcta aaaaggtctg agtgataagc gtgcttgata cgataaaagt ccctccaatg
     9001 attgctacta gtggaataaa cggatacatt ggaactttgt atggtcgaaa caaatcaggt
     9061 tcttttttac gaagaagaat gacaccaaca aagaccatcg tataaaaaat ccaaatgaca
     9121 aagatcagca tatccgttag tgtatcaaaa ccaccgatca tcatcatgcc aatagcgatg
     9181 actagctcta agatacctgc ggctacagga actttagttt tatcatgaag tttagcaaag
     9241 agcttgctaa aaggcaggtt gttttctttt gccataacat aagggagacg cattccagta
     9301 agggtatagc cattgatggt tccataaaca gaaatcaaga taccaactgt gaccagtctt
     9361 ccaccgaagc cgccaaaaat catttttgca acgtcacttg ccgcattgct gtttcctgcg
     9421 actccgtcga tagatgcggt tcttaagaac accgcattta ctaataaata aacgatcatg
     9481 attccgatga ttcctaaaga aattgctttt ggcaaatctt tcgccggctt tttcaattct
     9541 cccgaaatat ttcctacgtg tatccaaccg tcgtatgcga acatcgttgc tagcaagccc
     9601 gctcccaagg ctgagaaaaa tgaaagattt tctcctgctt ggatcggaaa cagttggaag
     9661 tctactcctt cttgtcggaa caaaccgaaa atgacgatca caaacaaagg aataagcttg
     9721 cagacaagcg taatcgactg aaaagcccct ccagctttag aacctaaaaa attgatcaac
     9781 aggatcgata cagcagctgt aacagctacg ggaacaatca ttgactggga aagaccaaat
     9841 agattgacga actgtgtacc gaaaatgatc gacaaggctg ctacatttgc tggaaaataa
     9901 atcacgactt gcgcccagcc caataaaaaa gctgctgtat ttccgtaaat ccgttcgata
     9961 tatttaatca tccctcctgt ttctggaata gcagctgcca gttcagctcc tgttagtcct
    10021 gcacagacag aaatcatacc accgagaaac caagaaaaca tatgcaaact ggcggatcct
    10081 gtaacttctg ctacgcttgc ggctttaaaa aatacccctg caccgatcac tgtgcccata
    10141 acagtcgata aagcagtaaa aaatcccatc gtacgtttta attcattttc tatttttact
    10201 tcttttactg gcgtggcttg atattcttcc atcccttgtc ccctttcata ccatagccaa
    10261 tcccaaaagt ataaaaaact ttttggatgt ggttcttgtt ttataccgtc tctaccaatt
    10321 tttctttcag tttttccaac atatcctttg tcatcacatc taagtcgtat ttcggcgaaa
    10381 agccccattc ctcttgcgca caagaaacat ctagtgaatt tggccaagat tctgctattg
    10441 actgacgtac aggatccaca tcataatcca tttcaaaatc tggtatgatt ttttgaatag
    10501 atgctttgat ctcttccggg tcaaaagaca tcgccgatat attgaaggca tttcgatgaa
    10561 ccaatcgatc aggatctgca ttcatgagtt ttataatggc atcgatcgca tctggcatat
    10621 acatcatatc cattgatgta ccttgatcaa tgaaactagt gtactttcct tctttgattg
    10681 ctgaataata aatatcgact gcgtagtctg tcgtaccgcc tcctggcaag gttgcataag
    10741 aaatcaatcc agggaaacgg acaccacgag tatctacgcc atatttcaca taataataat
    10801 cacatagtag ttctccagct accttcgtta ccccatacat ggttgtagga cgctgaagag
    10861 tatcttgtgg ggtatgatct tttggagtat ttggaccaaa cgcgccgatt gaacttggcg
    10921 taaaaaattt cagctggaat tctctagcga cttccaatgc attgaccagc ccagtcacgt
    10981 tcaaattcca ggctaattgt ggttttgctt ctgcaacagc tgacaaaaga gcagccaaat
    11041 gaatcaatgt gtccacttga tattgttctg ctatgctgcg cattttttcg taatccatta
    11101 catctaggat ctcaaagata tcgtttttct caatgacttc aggcatgcgt atatctgtcg
    11161 caatgacatt ttcactgcct agttcttgac gtagccgtgt gatcaactct gaaccaattt
    11221 gtcctaaaca tcctgtcacc aatacttttt tcatgatgat cctccctgat cttttttact
    11281 actatcttca gatgattaac aatatatgcg tgaacatgtt ctctaatttt aaatagacga
    11341 aaaaactaaa tttagttaaa taatttccat ttcttttcct attttttgat aaactgctac
    11401 tgcttcatca agcatttccc tcgtatgttc cgcagtcggc atatttctga tcctgcctgt
    11461 cccaagaggc acagttggat aaacgattgg tttagcataa atcccatgtt cgatcagctt
    11521 cttagagaaa gtctgagtca ctttttcgtc tcccaaaatg actggagtaa taggtgtttc
    11581 agacataccg atgttatagc ctactttttt caattcttct ttaaaataat tagcattttc
    11641 ccaaactttt tcgacaagtt ccggatgttc ttgcatcaaa gtaatcgaag ccaatgcagc
    11701 tgcagctgca cctggagtta gcgaagtaga aaataggaat ggccgacttc tagctttcaa
    11761 ccagtcgatt agtgttttcg atccagctac ataaccccca acaacaccta tagctttcga
    11821 taacgtgccc atttgcatat cgattttgtc gcttaatcca aaatgcttga cggttcctgc
    11881 accttttcca gttacgcctg agccgtgcgc atcatcgacg taggtgatca gtccatattt
    11941 ttcaacaatc ggaataattt ccggcaatcg tgcaatgtct ccatccatgg agaaaacacc
    12001 atctgtgata tacatgattt ttttatattt cttactttct atcgcttcct ttgctttttt
    12061 ctcaaggtct ttcatatctt gatgtttaat tcggatgatt tttgctccgg ataagcggca
    12121 accgtcaata atagatgcat ggtttaattc atccgataag atagcatctt cttttgtcat
    12181 tactgcagag atagcgccca tattgcagtt gaatccagat tgaaaggcga ttgctgcttc
    12241 tgtccctttg aattctgcga ttttttcttc taattgctga tggattttca gcgaaccatt
    12301 gatcgttcgg actgctcctg ctcctacacc ataagtttcg gttgcttcaa tacatgcttt
    12361 tttcaattct tcgcgattgg caaatccaag ataattgttg gatgctagat tgatcatctt
    12421 ttttccgtct acgatgatac aagcaccatt ttcactttct agtatgtcga tcgtattgta
    12481 taaccccttt tctttcagtt gatccaattc tttctctaaa tacattgtta gttctttgga
    12541 agacattgtc tccacctcca tattcatctg tcttgcagaa cataccaaac atatatcgtg
    12601 tcattcatct acaaagcaag tatgctatat ttttgataac gcttacactg attatgagaa
    12661 ttatttaacc acatttttta tttacagtat ctttttactt tcaaatgact tatatagtat
    12721 gaacaattac cttttagata atgatctatt ttactctacc tgggcagata aatacaacac
    12781 aaccattgat agaagccctt tcgttaaaat caattgtatt ctgaatattt aatttattta
    12841 ttgcatagac caataaaaat acataaaatt tccatcttcg tttatcagca atttttaatt
    12901 ttactctctg aatatttctt aaaagtttta cttgttgaat tcgaaaaaag gactgtgaca
    12961 aaagtcttgt cacagtccta ttcttccgaa ta
//
LOCUS       DASHHB010000069        12762 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.072, whole genome shotgun sequence.
ACCESSION   DASHHB010000069 DASHHB010000000
VERSION     DASHHB010000069.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12762)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12762)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12762
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.072"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U2K71_002371"
     CDS             complement(161..2158)
                     /gene="tkt"
                     /locus_tag="U2K71_002371"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HEM7316398.1"
                     /translation="MFDKIDQLGVNTIRTLSIEAVQKANSGHPGLPMGAAPMAYALWT
                     KHLKVNPTTSKNWADRDRFVLSAGHGSAMLYSLLHLAGYQVTIDDLKQFRQWDSKTPG
                     HPEVHHTDGVEATTGPLGQGIAMAVGMAMAEAHLAATYNKENFNVMDHYTYAICGDGD
                     LMEGVSQEASSMAGHMKLGKLIVLYDSNDISLDGPTSKAFTENVGARYEAYGWQHILV
                     KDGNDLEAISKAIEEAKAETDKPTLIEVKTVIGYGAPKEGTSAVHGAPLGEDGIKMAK
                     EVYGWEYPDFTVPEEVAARFHQTMIEEGQKAEDAWNEMFANYKKAYPELAQQFEDAFD
                     GKLPENWDAELPTYEVGSSQASRVSSKEVIQELSKAIPSFWGGSADLSGSNNTMVAAD
                     KDFTPEHYEGRNIWFGVREFAMASAMNGIQLHGGTRIYGGTFFVFVDYLRPAVRLAAI
                     QHTPVVYVLTHDSVAVGEDGPTHEPIEQLASVRCMPGVQVIRPADGNETRAAWKVAME
                     TTDAPTILVLSRQNLPVLPSTKEVADEMVKKGAYVLSPSQGETPEGILIATGSEVDLA
                     VKAQKELAEKGKDVSVVSMPSFDLFEKQSSEYKESVLPKSVKKRVAIEAAASFGWERY
                     VGTEGATITIDHFGASAPGTKILEEFGFTVENVVNTYNQLS"
     gene            complement(2260..2511)
                     /locus_tag="U2K71_002372"
     CDS             complement(2260..2511)
                     /locus_tag="U2K71_002372"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF896 family protein"
                     /protein_id="HEM7316399.1"
                     /translation="MLSPEKIERINELARKKKENGLSKAEEQEQINLRKEYLEAFRSG
                     MRHHIEGMKVVDPEGNDVTPEKLKEIQKKKGLHNRNDNF"
     gene            2658..3281
                     /gene="lexA"
                     /locus_tag="U2K71_002373"
     CDS             2658..3281
                     /gene="lexA"
                     /locus_tag="U2K71_002373"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010751062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HEM7316400.1"
                     /translation="MARQTESRQIEVLRYIHEQVTQKGYPPTVREIGEAVQLSSTSTV
                     HGHLARLEKKGFIQRDPTKPRAIELTKQGLEKIGIRPTTIPVLGVVTAGEPILAVEDA
                     SDFFPIPPNLSSEEGSLFMLTIRGDSMINAGILDGDNVIVRKQETAQNGDIVIAMTAE
                     DEATCKRFYKEKNFFRLQPENDLLEPIILDQVSILGRVVGLYRSHIY"
     gene            3461..3679
                     /locus_tag="U2K71_002374"
     CDS             3461..3679
                     /locus_tag="U2K71_002374"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316401.1"
                     /translation="MKSMRRLGLVLTVLFLGIIIGNFGIRPALLVFVPLFTLWFMLWD
                     EKKYRQSERRRIHEDHFYHEPYYRSRQS"
     gene            complement(3968..4480)
                     /locus_tag="U2K71_002375"
     CDS             complement(3968..4480)
                     /locus_tag="U2K71_002375"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HEM7316402.1"
                     /translation="MDLKDYIASIPDYPSKGIVFRDISPLMADGDAYREATKQIVNYA
                     KEKRIDMVVGPEARGFIVGCPVAYELGVGFAPVRKKGKLPRETIEVTYGLEYGTDTLT
                     LHKDAIKPGQRVLICDDLLATGGTIKATIELIEQLGGVVVGCAFLIELMDLHGRDKIK
                     GYDIITLMEY"
     gene            complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="U2K71_002376"
     CDS             complement(4494..6791)
                     /gene="recJ"
                     /locus_tag="U2K71_002376"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded-DNA-specific exonuclease RecJ"
                     /protein_id="HEM7316403.1"
                     /translation="MRQANFDWKLSETEPSESFLQMIRELKLSPFVGKLLWQRGYHEE
                     KDINRFLHPKEQELHDPYLIHDMDKAISRIQEAVISGEKILVYGDYDADGITSATVMK
                     ETLELLGAEVEVFLPNRFEHGYGPNQSVYQEKIEEGIQLIITVDNGVAGNDAVAYAQQ
                     AGVDVIITDHHELPENLPDAYAIVHPRHPAGHYPFPDLAGVGVAFKVASALLEEPPAE
                     FLDLVAIGTIADLVSLTDENRILVSLGIDAIHHSERIGLQALFEESGVKMRDADETTI
                     GFSIAPRLNAIGRMGDPRPAVSLLATFDEEEASVQAKKLNEINEERKAIVEQITQEAL
                     AMVNKENHIHLLVNRGWHEGVLGIVAGKIMNETGKPTLVLTLKEDGSAKGSGRSIEAL
                     NLFEMLDQMRDLFTYFGGHHAAVGLTMPSENVTILQEKMNQYIVDHQIDLMRGPELRI
                     DEVLLPNEVTVERIDELKLLAPFGTDNPLPQFLFRQVQAENIKRIGANQQHLKFVLSD
                     ASSQLDAVAFGFGTQEEELLNNQVDVVGKLSINEWNGRKKPQLMVSDFSVGGLQVFDW
                     RAKRFREQSRTSEGSLYLAFDSSTLKFVPEIIQEAAIVFEGLDSVKAIIEKRKPNSLV
                     VMDCPDDLADLKEVFRSYSFNRVYLMGISPDEAYLNGVGTREQYAKLFKLIHSQERID
                     IRHKIKAIAQYLRIPEKLLIFMIQVFFELKFVTIENGVLQKVASPESHPLTESRLYQR
                     RLNKIKVEEFLLLSDIPTIKKWLTT"
     gene            complement(6933..7376)
                     /locus_tag="U2K71_002377"
     CDS             complement(6933..7376)
                     /locus_tag="U2K71_002377"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1049 domain-containing protein"
                     /protein_id="HEM7316404.1"
                     /translation="MKELKKQSNVILGFVLVLIIVLFAVLNNKNVPVSFGFTSFSAPL
                     ILVIIGSALIGALIVFLTASATLWQQKKQIKQLTQEMSEYQTELQKKIEEAKEEQQRE
                     FRNERAELEAAYQAELQAKTAQISQLKAPEEQKTQSPKQSFNYFD"
     gene            complement(7377..8165)
                     /locus_tag="U2K71_002378"
     CDS             complement(7377..8165)
                     /locus_tag="U2K71_002378"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM7316405.1"
                     /translation="MNLEDKVVLVTGSSGGLGAQICYEAAKQGAIVISCARRMAFVEG
                     VRDECRRLSGKEAYAFKVDVSNPESVDELYEKVMEEVGRVDILVNNAGFGIFEDFLTF
                     DLGKAYDMFEVNILGMMVLTQKFAIDMAERRQGHIINIASMAGKMATAKSTVYSATKF
                     AVLGFSNALRLELKPLGVAVTTVNPGPIETNFFDKADPSGSYLEKVGQIVLEPAKLAK
                     MIVRNMRHPKREINQPFILEVASKFYTLFPTIGDYLASGIFNKK"
     gene            complement(8162..9103)
                     /locus_tag="U2K71_002379"
     CDS             complement(8162..9103)
                     /locus_tag="U2K71_002379"
                     /EC_number="3.1.26.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Z"
                     /protein_id="HEM7316406.1"
                     /translation="MELQFLGTGAGVPAKHRNVTSIALKLLDERNEVWLFDCGEGTQM
                     QILRTTIRPRKIGKIFITHLHGDHIFGLPGLISSRSFQGGDTPLEIYGPKGIEEYIKV
                     SLGISQTRLSYPLKFIELNETAPIFTDQQFSVYTKKLNHGIDSFGYRVVEHDHKGELQ
                     VDRLKELGIPAGPLYGKLKQGETIQLEDGRTINGKDFVGPDKKGRIVTILGDTRKTHN
                     SVVLAENSDILVHESTFNKDEARMAHNYFHSTTHQAAEVAKEAQAKRLLLTHISARYL
                     GKAALELEKEAQEVFENTTIMKDFDSIEIPFREEEEA"
     gene            complement(9280..9867)
                     /locus_tag="U2K71_002380"
     CDS             complement(9280..9867)
                     /locus_tag="U2K71_002380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1269 domain-containing protein"
                     /protein_id="HEM7316407.1"
                     /translation="MSKRVIILNFQVESQAYQAFSEIKKLHAVRSIKGEQMAVVSHDP
                     NGTHQFKIEDFIDFTGNNKSSTGGLIGMLVGVLGGPLGILLGWFAGGMIGATQDAKEI
                     KDAQTVFDFVGNKIGEGATGLILIADEEDNRPLNQLVMMELGGEITRLDYEDVEKDVH
                     DAKEAENHAKEAAQKTWKDKHPQTSNDNESSGSSN"
     gene            complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="U2K71_002381"
     CDS             complement(10088..11395)
                     /gene="obgE"
                     /locus_tag="U2K71_002381"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HEM7316408.1"
                     /translation="MFLDQVTIDVKAGKGGDGMVAFRREKYVPDGGPAGGDGGRGGDV
                     ILIVDEGLRTLMDFRFNRHFKAQPGENGMSKGMHGRGSEDTYVKVPQGTTVRDAETGA
                     LLGDLIENGQTLVVAKGGRGGRGNIRFASPRNPAPEIAENGEPGQERKIELELKVLAD
                     VGLVGFPSVGKSTLLSVISSARPKIGAYHFTTLVPNLGMVTTSDGRSFAAADLPGLIE
                     GASQGVGLGTQFLRHIERTRVILHVIDMSGMEGRDPYEDYLAINKELSTYNLRLLERP
                     QIIVANKMDMPDAPENLVKFKEQLNKEKEDEFADDIPVFPISGVTRQGLDALLNATAD
                     LLEVTPEFPLYEEELEEETVHYGFNPEGPEFQIDRDSDATWILSGEKIEKLFQMTNFD
                     HDETVMRFARQLRGMGVDEALRARGAKDGDLVRIGEFEFEFVE"
     gene            11689..12699
                     /gene="gap"
                     /locus_tag="U2K71_002382"
     CDS             11689..12699
                     /gene="gap"
                     /locus_tag="U2K71_002382"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HEM7316409.1"
                     /translation="MTVKVGINGFGRIGRLAFRRIKEVSDDIEVVAINDLTSPTMLAQ
                     LLQFDSTHGTYPGTVTATEDSIVVDGEATRVYAEPDASKIPWAKENGVDIVLECTGFY
                     TSEEKAKAHLDAGVKRVVISAPAGAMKTIVYNVNDDTLDENDKIISAGSCTTNCLAPM
                     AYFLNNEFGIEVGTMTTVHAYTSTQMLLDGPVKGGNLRAARSAADNTIPHSTGAAKAI
                     GLVIPELQGKLQGHAQRVPVVDGSLTELVSVLKTKVTADQVNEAIKKHTVDNPSFGYD
                     DRQIVSSDVIGTTQGSIFDPTQTEVTTAGDFQLVKTVAWYDNEYGFTCQMIRLLEKFA
                     NL"
BASE COUNT         3528 a         2858 c         2269 g         4107 t
ORIGIN      
        1 tcaagaaaca caaactaatc cgaagaattc tttgttttgt catttaaatg taacatagcc
       61 gaaaacgaaa agtaatattg atttattctt ctaacacata aagaacagct gtctccaact
      121 tgcagacagc tgttcttatc agtcaaacaa ttgcttggat ctatgatagt tgattgtacg
      181 tattcacgac attttctact gtaaatccaa attcttctaa aattttagtg ccaggggcag
      241 atgcaccgaa atgatcgatt gtaatcgttg cgccctctgt tcctacgtag cgttcccatc
      301 caaatgatgc agcagcttcg atagccacac gtttcttcac tgatttagga agtacacttt
      361 ctttatattc agaagattgt ttttcaaaga ggtcaaagct tggcatagaa acaacagaaa
      421 catctttgcc tttttcagcc aattcttttt gagctttcac agccaaatca acttcagaac
      481 ctgttgcaat caaaatacct tctggtgttt ctccttgtga tggagaaaga acatacgcgc
      541 cttttttcac catttcatct gctacttctt ttgtagaagg caatactgga aggttttgac
      601 gactcaatac aaggatagta ggcgcatctg ttgtctccat cgctactttc caagcagcac
      661 gtgtttcatt tccgtctgcc ggacgaatca cttgaacacc aggcatgcag cgaacagatg
      721 ctagttgttc aatcggttcg tgcgttggtc cgtcttctcc gacagcaacc gagtcatgtg
      781 ttaagacgta aacaacagga gtatgttgga ttgctgctaa acgaactgct ggtcttagat
      841 aatcgacaaa gacaaagaat gttccaccgt agatacgagt tccgccatga agttggatac
      901 cattcatagc cgaagccatt gcaaattcgc ggacaccgaa ccagatgttg cgtccttcat
      961 aatgttcagg agtgaaatct ttatctgcag ccaccattgt attattggaa cctgacaagt
     1021 ccgctgaacc accccagaag cttggtatag ctttcgataa ttcttggatg acttctttac
     1081 ttgaaacacg gcttgcttga ctgcttccaa cttcataagt tggcaattct gcatcccagt
     1141 tttctggtag tttgccatca aaagcatctt caaattgttg tgctaattca ggataagctt
     1201 ttttataatt ggcaaacatt tcattccatg catcttcagc tttttgtcct tcttctatca
     1261 tcgtttgatg gaagcgggca gctacttctt ctggaacagt gaaatctgga tactcccaac
     1321 cataaacttc tttagccatc ttgatgccat cttcaccaag aggagcacca tgaacagcag
     1381 atgttccttc tttaggagca ccatagccga tgactgtttt tacttcgatc aatgttggtt
     1441 tgtctgtttc agcttttgct tcttcaatcg ctttagagat tgcttctagg tcatttccgt
     1501 ctttcactaa gatatgttgc cagccatatg cttcatagcg agcaccaacg ttttctgtga
     1561 aagcttttga agttggaccg tctagtgaaa tatcgtttga atcgtataaa acgatcaatt
     1621 tccctagttt catatgacct gccatcgaac ttgcttcttg agatacccct tccatcaagt
     1681 ctccgtcacc gcatattgca tacgtatagt gatccatcac attaaagttt tctttgttat
     1741 acgttgctgc aaggtgcgct tctgccattg ccataccgac agccattgca atcccttgtc
     1801 ctaatggccc tgtagtggct tctacaccat ctgtatgatg tacttcagga tgtcctggtg
     1861 ttttagaatc ccattgacgg aattgtttca aatcatcgat tgtcacttga tagccagcta
     1921 aatgaagcaa gctatacaac attgctgacc catgtcctgc cgaaagaacg aaacggtcgc
     1981 ggtcagccca gttttttgat gtcgttggat ttactttcag gtgttttgtc cataacgcat
     2041 atgccatagg tgctgcgccc atcggcaatc caggatgtcc tgagtttgct ttttggactg
     2101 cttcaatact taatgtacga attgtgttaa cgccaagttg gtcaattttg tcaaacaaaa
     2161 taacccctcc tattaattta ggtttttttt acctttattt cttacacttt cattctataa
     2221 gatgtcacac gaaaaagcaa tctttttcag tattttttac taaaagttgt catttcggtt
     2281 gtgtaagcct tttttctttt ggatttcttt taatttttcc ggggtcacgt cattgccctc
     2341 aggatcgacg actttcattc cttcgatatg atgacgcata ccggaacgaa aagcttccaa
     2401 atactctttg cgcaagttga tttgttcttg ttcttcggct tttgagagac cattttcctt
     2461 ctttttacgt gcaagttcat tgatccgctc tattttttca ggtgacagca tgctcagccc
     2521 tccttttact ctccttcata ttatataaaa actgaaaaaa aaacaactag ataaactaaa
     2581 taaaatacga acattcgttt gatttagcgc tttttatatg gtactattta tttatcaagt
     2641 gaaagaaggt gtaccttgtg gcccgacaaa ctgaatcaag acaaatcgaa gtcttacgat
     2701 atatccatga acaagtaacc caaaaaggat acccgccgac tgtccgtgaa atcggggaag
     2761 ctgttcagct ttcttctact tctactgttc acggtcatct tgcacggctt gaaaaaaaag
     2821 ggtttatcca aagagatccg acgaaacctc gtgcaatcga gttgacaaaa caaggtttag
     2881 aaaagattgg gattcgtcca acaactattc cagtacttgg cgtcgtaaca gctggagaac
     2941 ccattttagc agtagaggat gcttcagact tctttccaat ccctcctaat ctttcttccg
     3001 aagaaggcag tctctttatg ctgacaattc gtggagatag tatgatcaat gctgggatat
     3061 tggatggtga caacgtgatc gttcggaagc aagaaactgc tcaaaatggc gatattgtca
     3121 ttgcaatgac tgccgaggac gaagcgactt gcaaacgatt ctataaagaa aaaaactttt
     3181 tccgtctgca gcctgaaaac gacttgctcg aaccaatcat cttagatcaa gtatctatat
     3241 taggacgagt agtaggactt tatcgtagtc atatctatta attgctacta attaaaagaa
     3301 aactttttaa aaagagtttg agacgttggt ctcaaactct tttttcgccc gggttcttct
     3361 tctttcaaat ttttatcact tccctcttta caaaccgaac aaatattcgt atactaaata
     3421 tacgaacaat tgttcgcata aattaaagag gtgagaattc atgaaatcaa tgagacgtct
     3481 tggcttagta ttaactgttt tgtttttagg tattattatt ggtaatttcg gcatacgtcc
     3541 agctttactt gtgtttgttc cgttatttac tttgtggttc atgttgtggg atgaaaagaa
     3601 gtaccgtcaa tctgagcggc gcagaattca tgaagatcat ttctatcatg agccctacta
     3661 cagatccagg caatcataat aaggattgac tgctaattat tcaaaagaca agaagttaaa
     3721 cttttacggg ggtgtgacaa aaatcggtca catccttttt ttcgaataaa cggtgttcta
     3781 aagccagttt ttcggtatta agtcaaattg cggaaaaagt gattttttaa acttctactg
     3841 gtctttgctt gtttccattt ataaaaaaat agcgaccctc aaaagtcaga tagagaatct
     3901 gacttttgag ggtcgcttta aaatcgcttc tttaagaata acatcaagaa tagcttcttt
     3961 tttctgctta atattccatt aatgtaatga tgtcatatcc tttgattttg tcgcgtccat
     4021 ggagatccat caattcgatc aaaaatgcgc agcctacgac tacaccgcct aattgttcga
     4081 tcagttcgat cgtcgctttg attgttcctc cagtagctag caaatcgtca caaatcagca
     4141 cacgttgacc tggtttaatg gcatccttat gcaatgtcaa tgtatctgtt ccatattcta
     4201 atccataagt cacttcgatg gtttctcgag gtaacttccc ttttttgcgg actggggcaa
     4261 aaccgactcc taactcataa gcaacaggac atcctacgat gaatccacgg gcttctggcc
     4321 caactaccat atcaattcgt ttttcttttg cataatttac gatttgttta gtagcttccc
     4381 gataagcgtc accatctgcc atcagtgggg aaatatctcg aaacacaatt ccttttgacg
     4441 gatagtcggg aatactggca atataatctt ttaaatccac tctttgtttc ctcctaggtt
     4501 gttaaccatt tctttatggt aggaatatcg ctcaaaagca aaaattcctc aactttgatt
     4561 ttattgagcc gtcgctggta taatctgctc tcagtcaaag gatgactttc tgggctagcg
     4621 actttttgta aaacaccgtt ctctattgta acaaatttca attcaaaaaa cacctgtatc
     4681 ataaaaatta ataatttttc agggatacgc aaatattgag ctattgcttt tattttatga
     4741 cgtatatcaa ttcgttcttg agaatggatc aatttaaaca attttgcgta ctgttctctt
     4801 gtgcctaccc cattcaaata ggcctcatca ggtgaaatcc ccatcagata aacacgattg
     4861 aatgaataag accgaaaaac ttctttcaga tccgctaaat catctggaca atccataaca
     4921 actagagagt ttggttttct tttttcaatt attgccttta cagaatctag tccttcgaaa
     4981 acaatcgcag cttcctggat aatctcaggc acgaatttca acgtcgaaga atcaaatgct
     5041 aaataaaggc taccttccga cgttcggctc tgttcacgga aacgttttgc ccgccaatca
     5101 aatacttgca gaccacccac agaaaaatca gataccatca gctgcggttt cttacgcccg
     5161 ttccattcat tgatcgaaag ctttcctacc acatctacct gattattcag cagttcttct
     5221 tcttgagtac caaaaccaaa agcgaccgca tcaagctgac tcgatgcatc ggaaaggaca
     5281 aatttcagat gctgttggtt tgctccgatt cttttgatat tttctgcttg gacttgtcga
     5341 aataaaaact gaggcaaagg attatccgta ccaaatggcg ccaatagttt cagctcatca
     5401 attcgctcta ctgttacttc atttggcagc aatacttcat caattctcaa ctcaggccct
     5461 ctcatcaagt caatttgatg atcgactata tattggttca tcttctcctg tagtatagtg
     5521 acattttcac ttggcatcgt taaaccgact gctgcatgat gtcctccgaa gtaagtaaat
     5581 aaatctcgca tctggtcaag catttcaaaa agattcaagg cttcaatact acgtccagat
     5641 cctttagctg atccatcttc ttttaaagta aggacaagcg ttggtttacc cgtttcattc
     5701 attattttcc cagcaacaat gcccaaaacg ccttcatgcc atcctcggtt taccaataga
     5761 tggatatgat tctccttatt gaccatcgct aatgcttctt gtgttatctg ctctacgatt
     5821 gcttttcttt cttcattgat ctcattcaat ttttttgctt gaacggatgc ttcctcttca
     5881 tcaaacgtcg caagtaacga gacggcagga cgtggatcgc ccatccgacc gattgcattt
     5941 agtctgggag caatcgaaaa accgatcgtt gtttcgtctg cgtccctcat ttttacgccg
     6001 ctttcttcaa atagcgcctg aagtcctatc cgttctgaat gatgaatggc atcgattcct
     6061 aatgaaacaa ggattcgatt ctcatcagtc aatgaaacta aatcggcgat cgttcctatc
     6121 gctactaaat ccaaaaattc tgcaggcggt tcttccaata atgcagaagc tactttgaat
     6181 gctactccga ctcctgccaa atctggaaat ggatagtgcc ccgctggatg acgagggtga
     6241 acaatcgcat aagcgtctgg taaattctca ggtagttcgt gatgatctgt aatgatcacg
     6301 tctacaccag cttgctgagc gtatgctacc gcatcatttc ctgctacccc gttatcaaca
     6361 gtgatgatca actggattcc ttcttcgatc ttttcttggt ataccgattg gtttggtcca
     6421 tacccatgct caaatcgatt tggcaggaat acttctactt ctgcaccaag taattccagt
     6481 gtttctttca ttaccgttgc actggtgatc ccatctgcgt cataatcacc gtaaacgagg
     6541 attttttctc cactgattac agcttcttgg attcggctga ttgctttatc catatcgtgt
     6601 atcagatacg gatcatgtaa ttcttgttcc tttggatgta aaaatcggtt gatatccttt
     6661 tcctcatggt aacctctttg ccacagtaac ttaccgacaa atggagataa tttcagttca
     6721 cggatcattt ggagaaagga ttcagaaggc tctgtctcgc ttaacttcca atcaaaattt
     6781 gcttgtctca ctttctcact cctaacttag ttattatagc aaaggaaatc agcaaaaaaa
     6841 agcagaaaga aagaataaaa acagatcaga tccgctagac agatgttcag caggacctga
     6901 tcttttgtac gaccatttat gaaatatccc tattagtcaa aataattgaa gctttgtttg
     6961 ggcgattgag ttttttgttc ttccggcgct ttcaattgac tgatttgtgc ggttttcgct
     7021 tgtaactccg cctgataagc agcttccaac tccgcacgtt cgtttctaaa ctcgcgttgc
     7081 tgttcttctt ttgcttcttc aattttcttt tgaagttctg tttgatattc tgacatttcc
     7141 tgagtcagct gtttgatttg ctttttttgc tgccatagtg ttgccgaagc agtcaaaaat
     7201 acaatcagtg caccaatcag cgcagaaccg atgatcacta agatcaatgg cgcagaaaag
     7261 gaagtgaagc caaaacttac aggaacattt ttattgttca atacagcaaa aagaacaata
     7321 atcaaaacta aaacaaatcc aagaatcaca ttgctttgct ttttcaattc cttcacctac
     7381 tttttgttaa aaattccgct ggctaaataa tcaccaatcg ttggaaacag cgtataaaat
     7441 tttgatgcaa cttctaaaat aaacggttga ttgatttccc gttttggatg tctcatattg
     7501 cggacgatca tttttgccaa cttggcaggt tctaaaacga tttgtccgac tttttccaaa
     7561 tagcttccgc ttggatcagc cttatcgaag aaatttgtct cgattggacc tggattcact
     7621 gtcgtaacag ctacgccaag tggtttcaat tctaatcgca aagcgttgga gaagccaagg
     7681 actgcaaatt tcgtggcaga ataaaccgtt gattttgcag ttgccatttt tccagccata
     7741 gaggcgatat taatgatatg accttgtctt ctttctgcca tatcgatcgc aaatttttgt
     7801 gtcaaaacca tcattcctaa gatgttgact tcaaacatat cgtatgcttt tccaaggtcg
     7861 aatgtaagga agtcttcgaa aatcccaaaa cccgcattgt ttaccagaat atcgacgcgc
     7921 ccgacttctt ccatcacctt ttcatataat tcgtccacgc tttcaggatt gctgacgtct
     7981 accttgaaag cataagcctc tttgccgctc aagcgtctgc actcgtctct gacaccttct
     8041 acaaatgcca ttcttcgtgc gcaacttata acgattgctc cttgctttgc tgcttcataa
     8101 cagatttgtg cgcccagacc gcctgaacta cctgttacta aaacaacttt atcttctaaa
     8161 ttcatgcctc ttcctcctcg cgaaatggaa tctcaatgct atcaaaatct ttcataatcg
     8221 tcgtgttttc aaacacttct tgtgcttctt tttcaagctc taaagctgct tttcctaaat
     8281 atctcgcact gatatgagta agtagcagcc gttttgcctg cgcttctttt gcaacttcag
     8341 cagcttgatg agtcgtggaa tggaaatagt tgtgcgccat tcttgcttca tctttattaa
     8401 atgtgctttc atgtaccaga atatcgctat tttccgcaag cacaacggaa ttatgtgttt
     8461 ttctcgtatc gcctaaaata gtcacgattc gtcctttttt atctggacct acgaaatctt
     8521 ttccattgat cgtccgccca tcttctagtt ggatcgtttc accttgcttc agtttgccgt
     8581 acagtggtcc agctggaata cctaattctt ttagacgatc cacctgcaat tctcctttat
     8641 gatcatgttc aaccacacga taaccaaaac tgtcgatccc atgatttagt ttttttgtat
     8701 aaacagaaaa ttgctgatca gtaaaaatcg gggctgtttc atttagctca atgaatttca
     8761 gcggataact taaacgcgtt tgcgaaatac ctaaagagac ttttatgtat tcctctatcc
     8821 cttttggccc gtaaatctct aatggcgtat ccccaccttg aaatgaccgg ctgctgatga
     8881 gcccgggaag tccaaaaata tgatcgccgt gtaaatgtgt aatgaaaatt ttaccgattt
     8941 ttctcggacg aatagtcgtt cgcaaaatct gcatttgcgt gccttcgcca cagtcgaaca
     9001 accaaacttc attgcgctcg tctaaaagtt tcaatgctat acttgtcaca tttctatgtt
     9061 ttgcaggaac acctgcacct gttcctaaaa attgtaattc cataatctac ctgactttct
     9121 ttaagttcct ctttaatttt agggtaaaaa gaagggataa tcaaccgtaa gtcgagaatg
     9181 ggttcaaatt aaaaataacg ttctaaagcg atagcgtttt ctttgaaaat tactataaaa
     9241 acagcagcga acccgtttga gatcctctgc tgttttttat taatttgaag atccagaact
     9301 ttcattgtcg ttggaagttt gcggatgttt gtccttccaa gtcttttgtg cagcttcttt
     9361 cgcatgattt tcagcttctt tcgcatcatg cacatctttt tcgacatctt cataatccaa
     9421 gcgagtaatt tctccgccta attccatcat taccaattga ttcagtgggc gattgtcctc
     9481 ttcatcagca atcaagatca gtcctgtagc tccttcaccg attttatttc ctacaaagtc
     9541 gaacacggtc tgggcatctt ttatttcttt ggcatcttgt gtcgcaccga tcattcctcc
     9601 ggcaaaccat cctaataaaa tccctagcgg accacctaat acaccaacca acattccgat
     9661 taatccaccc gtagaagatt tgttattacc ggtaaagtcg ataaaatctt ctattttgaa
     9721 ttgatgtgtc ccattaggat catggctgac aactgccatc tgttctcctt taatcgaacg
     9781 aacagcatgt aactttttga tttctgaaaa tgcctgatat gcctggcttt ctacttgaaa
     9841 atttaaaatg attactcgtt tgctcattaa aaacgcccct ttcatacctt cttgttattt
     9901 attttacctt taattgaaaa ataagaaaag aaaagcactc tttttttcca ataaaatagg
     9961 gctgtgacaa aagtcgtatc acagctcctt tttccgaata aacggcgttc ttgaagctga
    10021 tcacttctgt cacaacctcc cttaaatctg aatctatatt ttttggtatt tgtttttatt
    10081 tgcttgccta ttcgacaaat tcaaattcaa actcaccgat acgtacaagg tcgccatctt
    10141 tcgcaccgcg tgcccgaaga gcttcatcca cacccatacc acgtaattga cgagcaaaac
    10201 gcatcactgt ttcatcatga tcgaaattcg tcatttggaa taatttttcg atcttctcac
    10261 cagataagat ccacgttgca tctgaatcac gatcgatttg gaactcagga ccttctggat
    10321 tgaatccata gtgtacagtt tcttcttcca gttcttcttc gtacaatggg aattctggcg
    10381 ttacttctaa caaatctgct gtagcattca ataacgcgtc caatccttgt cgagtcactc
    10441 cagaaattgg aaatactggg atgtcatcag caaattcatc ttctttttcc ttgttcagct
    10501 gttctttgaa tttcactaga ttttccggtg catccggcat atccattttg tttgccacga
    10561 tgatttgcgg acgctctagc aagcgtaagt tataagtaga taattctttg ttgatggcaa
    10621 gatagtcttc ataaggatct cgtccttcca tcccactcat atcgatcaca tgtaaaatta
    10681 ctcgtgttct ttcgatatga cgaaggaatt gtgtacccag cccaacacct tgcgaagctc
    10741 cttcaatcaa gcctggcaag tctgctgcgg caaaacttct gccatcactt gtagtaacca
    10801 ttcctagatt aggtacgagc gtcgtgaaat gataagcgcc gatttttgga cgagcagatg
    10861 aaataacgga tagtaaagtc gatttcccga cagatggaaa acctacaagt ccaacgtctg
    10921 ccaatacttt caattctagt tcgatttttc gctcttgtcc tggttcaccg ttttcagcaa
    10981 tttccggtgc aggattccga ggagaggcaa aacggatatt ccctcgtcca ccgcgtccac
    11041 cttttgccac aactagtgtt tgaccatttt caattaaatc tcctaaaaga gcaccagtct
    11101 ccgcatctcg gacagtggtt ccttgtggta ctttaacata ggtatcttct gaaccacgac
    11161 cgtgcatccc tttactcatc ccattttcac ctggctgcgc tttgaaatgg cgattgaacc
    11221 gaaaatccat caatgtacgt aacccttcat cgacgatcaa gatgacatcg ccgccacgac
    11281 caccgtctcc gccagccggt ccgccgtcag gaacgtattt ttcgcgacga aatgcaacca
    11341 ttccgtctcc gcccttgcca gctttcacat cgatcgttac ttgatctaaa aacatggaca
    11401 taattaagtc ctcctattat tcatttattt atttgactgc ctgaatttcc acaataaaca
    11461 aaagtcttac ttattttatc ggtgaaatct gattttgtct atcatttcag caaaaagatt
    11521 gtttaccttc ttcttttttt atcatttggt aaggtttcca accttttttc tgctaccaag
    11581 tataattttt tgacaaaata ttctttgcgt agcagaataa cagtgtatta attgtattaa
    11641 ttgaggatgc cgtttcacag cacccctaat aaaggaggaa cttttgatat gacagtaaaa
    11701 gtaggtatta acggatttgg gcgtattggc cgtctggctt tccgccgcat caaagaagtc
    11761 tcagatgata ttgaagtagt agcaatcaac gacttaacaa gtccaactat gttagcacaa
    11821 ttattgcaat ttgactctac tcatggtact tatcctggca cagttactgc aacagaagac
    11881 tcaatcgtag tggatggcga agcaactcgt gtttatgcag aacccgacgc aagcaaaatc
    11941 ccttgggcaa aagaaaacgg tgtagatatc gtactagaat gtactggttt ttatacatct
    12001 gaagaaaaag caaaagcgca tttagatgct ggtgtcaaac gagtagttat ttctgcccca
    12061 gctggtgcta tgaaaaccat cgtctataac gtcaatgatg atacattaga tgaaaacgac
    12121 aaaatcatct cagcaggttc atgtacaaca aactgtttag cacctatggc ttatttctta
    12181 aataatgaat ttgggatcga agttggtaca atgacaacag ttcatgccta cacttccaca
    12241 caaatgctct tagatggacc tgtaaaaggc ggtaatttgc gtgctgctcg ttcagctgct
    12301 gacaatacta tcccacattc tactggtgct gcgaaagcaa tcggcttagt tatccctgag
    12361 ctacaaggaa aattacaagg acacgcacaa cgtgttccag tcgttgatgg ttcattaact
    12421 gaattggttt cagtattgaa aacaaaagta actgctgatc aagtaaacga agcaatcaaa
    12481 aaacacacag ttgacaatcc ttcattcggg tatgatgacc gtcaaatcgt ttctagcgat
    12541 gtcattggaa caactcaagg ttcgatcttt gacccaacgc aaacagaagt aacaactgct
    12601 ggtgatttcc aacttgttaa aactgttgca tggtatgaca atgagtatgg atttacttgc
    12661 caaatgattc gcttactaga aaaattcgct aacttgtaat ttgatagatc aatcgataaa
    12721 agaagccttt gactggatcc agtcaaaggc ttctttcttt tc
//
LOCUS       DASHHB010000070        12740 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.073, whole genome shotgun sequence.
ACCESSION   DASHHB010000070 DASHHB010000000
VERSION     DASHHB010000070.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12740)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12740)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12740
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.073"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            99..542
                     /locus_tag="U2K71_002383"
     CDS             99..542
                     /locus_tag="U2K71_002383"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM7316410.1"
                     /translation="MDKRQLLIEEYLDLANEIIHRNKPEMEKEFAGFTLNELEVIEHI
                     GKIPDPNVTKLANASYMTRGAISKLTKKLIARGIINTYQSEENKKEIYFQLTPIGQKI
                     RNRHEQLHQKWVQRDATVFENMSKEEFDTVFRFIGRYREFIRSSK"
     gene            complement(548..1732)
                     /locus_tag="U2K71_002384"
     CDS             complement(548..1732)
                     /locus_tag="U2K71_002384"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance MFS efflux pump"
                     /protein_id="HEM7316411.1"
                     /translation="MYSFTNKHSKVFGYVSVLFTGLGFTIVSPVLPFLTLPYSHSIHQ
                     QAFYITLLMSVYALAAFLSAPILGSLSDHFGRHPILIISLLGSSIGYLLFGLGNSIWM
                     LFLGRIIEGLTAGEISTLYAYFADITEPNERTKVFGWMGALVGIGTTLGPIIGGLLAE
                     LGNSVPIFIGALFTFLNAVYGYTFMLESLPIKKRSVDLSFSHVRPFHQLQQLFKISSV
                     IPLLTAGFAVWLAAGSLQSIFSQFSIDAFQWKAGLVGLSFSLIGILDTVSQLFIMPRL
                     LKKFSEQQITRIGMFSEILAYLFITLSGILLLPILFLFGIICYGFGDSIFTPVFNGQL
                     SNSVSDNQQGLVMGGTQSIQSLSRVIGPLIAGQLYAAAPFLPTVFGFLLMIFAYYHYN
                     KK"
     gene            2000..2698
                     /locus_tag="U2K71_002385"
     CDS             2000..2698
                     /locus_tag="U2K71_002385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HEM7316412.1"
                     /translation="MLNLENYLSAGQTVISNLLLKNYHRIGLQSEEFLFVLQLHMAQL
                     EGDTFPDLQMIAMDMGIKQDQIFQILDRLVTNGFIKIETTIVNGKKADRYNLYPIYDL
                     LGEYLKTQEKKYEEKTQEKEIQSVYQLFEQEFGRPLSSIEFQRIGQWLEEDHYQPEIL
                     KLALREAVLNQAYSFNYVDRILLSWERKNLRTKQQVEEDQKRRKQQLLQREVEQSAQQ
                     EELPKVSLKNWLEE"
     gene            2705..3382
                     /gene="nth"
                     /locus_tag="U2K71_002386"
     CDS             2705..3382
                     /gene="nth"
                     /locus_tag="U2K71_002386"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease III"
                     /protein_id="HEM7316413.1"
                     /translation="MLSKQKTMEALETMYGMFPEAHGELKHNNPFELLIAVILSAQAT
                     DVSVNKATPDLFASFPTPDALAEASIDEIILKIKTIGLYRNKAKNIKACAQQLIERFD
                     GQVPTSREELMSLPGVGRKTANVVLGDAFGIPAIAVDTHVERVSKRLRICKLDATVME
                     VEETLMRKVPQELWVKTHHTLIFFGRYHCTARNPKCEVCPLLSICQDGKNRMRLKEKA
                     LKKKPRL"
     gene            complement(3508..4041)
                     /locus_tag="U2K71_002387"
     CDS             complement(3508..4041)
                     /locus_tag="U2K71_002387"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316414.1"
                     /translation="MVLNNFLKEGFILSYLIVCLIIIICLHHLFLSKTIPRYSKNKKA
                     EKFCLLLNKFTLIAPILAFIIFSVLLSTTLKGKFMERSSHAMILTFLWLLFTRIYIFL
                     MSLKPPKSISLCLVINGIFLLSLIIFITPLDRYVTYLYNPLEYWTYFIGILEGIIFYI
                     GYFPNKNNNFYFYRNKL"
     gene            complement(4338..6719)
                     /locus_tag="U2K71_002388"
     CDS             complement(4338..6719)
                     /locus_tag="U2K71_002388"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PBP1A family penicillin-binding protein"
                     /protein_id="HEM7316415.1"
                     /translation="MANEQTRSSRRQKQPTPKKSVKKNSGKGSGKSSGTHKKGLFIKI
                     LLGILSFFCILFLAGVGLFWYYAKDAPELTDKKLDATVSSKLYTQDGELFEDLGAEKR
                     EKISANELPKTLEDAIVSVEDRRFYKHIGVDPIRIIGSALSNFTSGGLQGGSTLTQQL
                     IKLSFFSTSAEDQTLKRKAQEAWMAVRLEQKKSKQEILTYYVNKVYMSNGLYGMETAS
                     EMYFGKKLSELSLPQTALLAGMPQAPSAYDPYVYPDQAKKRRDTVLYTMLQNEKISQT
                     EYDQAVNVPVTDGLQELTQSDDNTKIVDNYVKEVINEVQEKTDKNVYTDGLEIYTNLD
                     LDAQKKLYDIVNTDQYVSYPDDEMQVASTLIDTNTGKVKAQIGGRHIAEDVTLGNNLA
                     VNTSRDFGSTMKPVTDYGPAFEYLKYSTGKTITDAPYNYEGTSTPVGNWDNQYMGTIT
                     LRQALYLSRNVPAVKLFNEVGSDKVASFLKNLGIEYSTIHQSNAISSNTEEQDGTKYG
                     ASSLKMAAAYAAFANGGTYYKPQYVNKIVFQDGTEETYEPDGKTAMSPETAYMITDIL
                     KDTITEGTGTNAQIAGLYQAGKTGTSNYTDDEYAKLGISSGVYPDILFAGYTPNYSIS
                     VWTGYNKKMTPVTSESSHVASDVYRELMQYVSANVTNTDWEMPSGLIRVGGELYYKDQ
                     YTARSNAITPSTTIPSSSYVQTPGSSTTETTTQSSSSTSQSESTAESSKESTTAETSE
                     PASSTTVPSSSSEESSTPSSSAPPASSSEPATSGADAANDHTPSSSTSASGNR"
     gene            complement(6709..7356)
                     /gene="recU"
                     /locus_tag="U2K71_002389"
     CDS             complement(6709..7356)
                     /gene="recU"
                     /locus_tag="U2K71_002389"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005875836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction resolvase RecU"
                     /protein_id="HEM7316416.1"
                     /translation="MVLRYPNGQPYYKNASPVEKIQEKRNQITEFGNRGMRFEEAINE
                     SNKYYLSRGLAVIHKKPTPIQIVKVDYPKRSAAVIKEAYFTQASTTDYNGVYRGFYLD
                     FEAKETKNKTSFPFKNFHAHQIEHMKACLEQNGVCFVLLWFSSLKRCFYLNSVHLIAY
                     WDAQKEKGRKSLPLSLIEELGIEIKTGISPRIPYLDAVDFYLSTLSIEGESKNGK"
     gene            7420..7959
                     /locus_tag="U2K71_002390"
     CDS             7420..7959
                     /locus_tag="U2K71_002390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1273 domain-containing protein"
                     /protein_id="HEM7316417.1"
                     /translation="MDKSKVIYVSGYRSFELGVFKENDPKIQVVKNVLKKELHQLAEE
                     GLEWVLVSGNLGVELWAVQIVAELKEEYPELSLGILYPFSDFGNNWNEGNQEKKQLAE
                     QLADYVEAVSHQPYQSPSQLKNHTRFILEHTVGCLLIYDEEFPGKTKYFFEDAQNYQA
                     DHPYEIRLISMDDLQNFSE"
     gene            8049..8468
                     /gene="gpsB"
                     /locus_tag="U2K71_002391"
     CDS             8049..8468
                     /gene="gpsB"
                     /locus_tag="U2K71_002391"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division regulator GpsB"
                     /protein_id="HEM7316418.1"
                     /translation="MANLVYSPKDILQQEFKTKMRGYDPVEVDEFLDNIIKDYETYSK
                     ELLALQEENDRLSAKVAQLSKTQGAAQTRVQQTEAPKSAAVTNFDILKRLSNLEREVF
                     GKKLDQQASAVKPAQPNPNNYTNADTSLDDNEKTRQF"
     gene            8490..8857
                     /gene="rnpB"
                     /locus_tag="U2K71_002392"
     ncRNA           8490..8857
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="U2K71_002392"
                     /product="RNase P RNA component class B"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00011"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00011"
     gene            9066..10232
                     /locus_tag="U2K71_002393"
     CDS             9066..10232
                     /locus_tag="U2K71_002393"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HEM7316419.1"
                     /translation="MEKNRKFNLVATAASGLEALVGKELRDLGIECQVENGRARFEGT
                     METIATANLWLRTADRVKIVVGEFDAYTFDELFENVKALPWEDFLPLDAAFPVAGKSI
                     KSKLYSTPDCQAITKKAIVERLRTYYHRPASVPLTETGAHFQLEVALLKDHVMVTLDT
                     TGPSLFKRGYRLEKGGAPLKENMAAALIMLTNWRKDRPFYDPVCGSGTLCIEAALIGH
                     NIAPGFNREFACESWDWFSQEIMENVRSAAEEKADYDIELDITGSDINGRMIEIAKAN
                     AEEIGLGSSITFKQQAVKDFKTDKEYGVIVANPPYGERLGEEETVRKLYKEMGDVFRP
                     LKTWSKYILTSDLAFEEFYGQKATKKRKLYNGALRTDLFQYWGTRPPRKKEKDE"
     gene            10278..11774
                     /locus_tag="U2K71_002394"
     CDS             10278..11774
                     /locus_tag="U2K71_002394"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010738715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HEM7316420.1"
                     /translation="MKEKEFLQEVKEINLLQQALGILDWDTQTGMPEKASAYRSEVDS
                     YLYSIYFSKKIGPKIKEAIRYFSEHPEELSEVGTAVFAKVKEEYDLEHRVPEDLMRKY
                     TAAVSSAHGQWQKARETQQFSDFQHALNQNIELTKQLIPYWRKDEKTNYDVLLNQYEP
                     GMTVEILDKVFDQLKEGILSIRRTLQEKGTEPETDFLNRRMTKKQQKRFVVKVIEQLG
                     YDFSRGRLDDTVHPFMTGINPNDARITTRWNETDFKMAVFGVIHEAGHGMYEQDIDDK
                     YAYTPVYEGTSMGIHESQSLFNEIIIGSSRSFWKKQYPFFQECAEGTFDDIPFDKFYR
                     SLNHTKSSLVRIEADSLTYPLHIIIRYEIEKMIFNEGVDVAELPEIWNQKYEEYLGIR
                     PTNDVEGILQDVHWSGASFGYFPSYALGYMYAAQLLHSLQQEIDVENVLASDDYSPIK
                     NWMSEHIHQYGASRKPNQLIMDATHEPLNPQYLIDYMKDLYFNVYQVK"
     gene            11855..12538
                     /locus_tag="U2K71_002395"
     CDS             11855..12538
                     /locus_tag="U2K71_002395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM7316421.1"
                     /translation="MDKLQVIADYSMDQLAHDQTGHGSDHTKRVVKLAEKILETEPQA
                     DRFVTLAAAYLHDTIDDKVVKNENEAKQQLRVFLRSLPIVEEQISMIFSIIENMSFSK
                     NLSEAAELSLEGKIVQDADRIEALGAIGILRTAYFGGGHGHPIFDPELYPQTFKDKEN
                     YRKGTTVINHFYEKLFLLSDKMNTDYGYEEAKRRETFMREFLEEFFLEWSIDDENFTP
                     DNWIKNNKK"
BASE COUNT         4273 a         2230 c         2630 g         3607 t
ORIGIN      
        1 tttcttggtt tcagattggg ttttcctaca aaatattttt atctttgttg aataggaaac
       61 aaaaaaacaa tataatgaaa gaaaaagaag gtgtagaaat ggataaaaga caactgctaa
      121 tagaagaata tctggattta gctaatgaga ttattcatcg taataaacca gaaatggaaa
      181 aagaatttgc aggatttact ctaaatgaac tagaagtgat tgaacatatc gggaaaatcc
      241 ctgatcctaa cgtgacaaaa ttagcgaatg ctagttacat gactagagga gccatcagta
      301 agctaacaaa aaaactgata gctagaggga tcataaacac ttatcaaagc gaagagaata
      361 aaaaagaaat ctattttcaa ttgacgccaa tcggacaaaa aataaggaat agacacgagc
      421 aactccatca aaaatgggta cagcgagatg ctactgtatt tgaaaatatg tcaaaagaag
      481 aatttgacac agtatttcgt tttatcggtc gatacagaga atttatacga agtagcaaat
      541 gaagctacta ttttttattg tagtgataat aggcaaaaat cataagcaaa aatccgaata
      601 ccgtcggaag aaatggtgct gcagcataaa gctgtcctgc gattagcggg ccgatgacgc
      661 gcgatagaga ttgtatactt tgggtaccgc ccattacaag accttgctgg ttatctgaga
      721 cagaattgga tagttggcca ttgaatacag gagtaaaaat agaatcaccg aaaccataac
      781 agataatccc aaataaaaac aaaatcggca gtagaagaat tccagataat gtgatgaata
      841 gataggcgag gatttcactg aacatcccta ttcttgtgat ctgctgttct gaaaattttt
      901 tgagcaatcg aggcataatg aacaattgcg agacagtatc caagattcca atcaaagaga
      961 agcttaaacc aaccaaccca gccttccact gaaaggcgtc aatcgaaaat tgagaaaaga
     1021 tagattgaag agagccagct gccaaccaaa cagcaaaacc tgctgtcaat aaaggaataa
     1081 cggatgaaat cttgaataat tgttgtaatt ggtgaaaagg tcttacatgg gagaaactta
     1141 aatctactga acgttttttt attggaaggc tttctagcat aaatgtgtag ccataaacgg
     1201 catttaagaa agtaaagagt gctccaataa atataggaac agaattcccc aactcagcta
     1261 atagaccccc tataatagga cccagcgttg ttcctatacc gactaaagca cccatccatc
     1321 caaaaacttt ggttctttca ttaggttccg tgatgtcggc aaagtaagca tataaggtgg
     1381 agatttctcc tgccgtcaat ccttcaatga tacgaccaag gaacaacatc catatcgaat
     1441 ttcctaaacc aaaaagcaaa tatccgatac ttgatcctaa taaactgata ataaggatcg
     1501 gatgacgccc aaaatgatca cttaagctac cgagaatggg ggcagataag aaggcagcta
     1561 gagcataaac agacatgagc aaagtgatat agaaagcttg ctgatgtatg gagtgagaat
     1621 aaggcagcgt taaaaacgga agtacaggac tgacgatagt aaatcctaat ccagtgaata
     1681 agacagagac gtatccaaaa actttagaat gtttatttgt gaaactgtac atgtaaaaac
     1741 tcctttatgc aataatgtta acttggaaac aaaaatagtg tatagtaatt ttgtttcctt
     1801 gtcaacattc taaggaacag cgaaatctac ttaatttatt tattccgaaa agtgaagata
     1861 cgcatcaaaa acatgaacaa acaaaaatca agtagacgac attgtaaatc tgaccgatca
     1921 gtgaagtaaa tagaaaagaa acctgttctt gcttcccatg ttttttttac gtatactgtg
     1981 attgagcgga ggagaaatca tgttgaattt agaaaattac ttaagcgctg gacaaacagt
     2041 catatctaat cttttactta aaaattatca taggatcggt ctacaatctg aagaattttt
     2101 atttgttcta cagttgcata tggcacagct tgaaggagat actttccctg atttgcagat
     2161 gattgctatg gatatgggaa tcaaacaaga tcagattttt caaatactgg atcgtctcgt
     2221 aacaaatgga tttatcaaga tagaaacgac gattgtcaac ggtaaaaaag cagatcgata
     2281 taatctttac cccatctatg atctattagg tgagtattta aaaacacaag aaaaaaaata
     2341 cgaagaaaaa acacaagaaa aagaaatcca gtcagtgtat caattatttg aacaagaatt
     2401 tggacgcccg ctttcttcga tcgagttcca gcggatcgga caatggctag aagaagacca
     2461 ttatcagccg gaaattttga agctagcact gcgggaagcc gtattaaatc aggcttatag
     2521 ttttaattac gttgaccgca ttttgctatc gtgggaaaga aaaaatctac gtacaaaaca
     2581 acaagtggaa gaagaccaaa aacgaagaaa acagcagctt cttcaaagag aagtagaaca
     2641 gtcagctcaa caagaagaat tgccgaaagt ctcactgaaa aattggttgg aggagtagcg
     2701 gaatatgtta agcaaacaaa aaacaatgga agctctagaa acgatgtatg gaatgtttcc
     2761 cgaagcacat ggagaactca agcacaataa tccatttgag ttattgattg cagtgatctt
     2821 aagtgcccag gcgacagatg tatcagtgaa taaagcaacg ccagatttgt ttgcctcttt
     2881 tccgacaccc gatgcgttag cagaagcttc aatcgatgaa atcattctta aaataaaaac
     2941 gattggtttg tatcgaaata aagcaaaaaa catcaaagcg tgtgcacagc agctgattga
     3001 acgtttcgat gggcaagtgc caacgtcaag agaagaattg atgtctcttc ctggtgtagg
     3061 cagaaagaca gcaaatgtcg tattggggga tgcattcggg ataccagcaa tcgcagtaga
     3121 tacccacgtg gaacgtgtct caaaacgcct gaggatctgc aaactagatg ctacggtcat
     3181 ggaagtagaa gaaacactga tgcggaaagt accacaagaa ttatgggtaa agacacatca
     3241 tacactgatt ttttttggta gatatcattg cacggcaaga aatcccaagt gcgaggtatg
     3301 tcctttgcta tcaatatgtc aagatgggaa aaatcggatg cgtctaaaag aaaaggcgct
     3361 aaaaaagaaa ccacgcctgt agacgtggtt tctttaattg caaataaatg tatgaattat
     3421 cttgttatta tggagaaaag ctgtttcgtt tatctttttt gcatgaaaaa gtaaatgtac
     3481 atactcagta gctaggatgg taacttatta caatttattg cgataaaaat aaaagttgtt
     3541 atttttattg ggaaaatacc cgatgtagaa aataatgcct tccaatatac ctataaaata
     3601 agtccagtat tccaaaggat tatataaata agtgacgtat ctatctaaag gtgtaataaa
     3661 aatgataagt gataataaaa aaataccatt tataactaaa cataaactaa tggatttggg
     3721 cggtttcagg ctcatcagaa aaatatagat tctggtgaac aacaaccata aaaaagtgag
     3781 aatcattgca tgagagcttc tttccataaa ttttccttta agagtagtgc ttaaaagtac
     3841 agaaaaaata ataaatgcaa ggataggagc aattaaagta aatttattta ataataaaca
     3901 aaatttttca gcttttttat tcttggaata acgaggtatt gttttcgata aaaaaagatg
     3961 gtgcagacaa attatgataa ttaagcatac tatcagataa ctcaaaatga agccttcttt
     4021 caaaaaatta ttcaaaacca tatattgtat tttttatttg ataataacaa tgggattatt
     4081 aagaagtggc actaaataaa agactagtgt gtatttagcc gatttaatta taaacaagta
     4141 aatggataaa gtatatgtaa tgataaaaga aaaatggatt ttctaatttt taagctaaat
     4201 gttagatcac acatgtatat gctggatagt cttataaaaa atcatgtaat acagcttggt
     4261 acatgatttt tatttgtagc tgttttttac atagacacaa aaaagaaacc acgcttgtag
     4321 acgtggtttc ttttttacta tctgtttcca gaagctgagg tgctgctgga tggtgtatgg
     4381 tcatttgcag catccgctcc gctagttgct ggctcactgg aagaagcagg aggagcagat
     4441 gaagacggtg tcgagctttc ttcggatgaa gaagaaggaa ccgttgttga agacgctggc
     4501 tcgcttgttt ctgctgtcgt gctttctttt gaggactcag cagttgattc gctttgacta
     4561 gtagagctag agctttgagt cgttgtctct gttgttgagc ttccaggagt ctggacatag
     4621 ctggaagatg gaatagttgt tgatggtgta attgcattcg atctagctgt atattgatct
     4681 ttgtagtaca gttcaccgcc cacacgaatt aatccgcttg gcatctccca gtctgtattt
     4741 gtcacgttag ctgaaacata ctgcataagc tcgcgataaa catcagaggc tacgtgtgac
     4801 gattctgaag tgacaggcgt cattttttta ttgtaacctg tccacacgga aatcgaataa
     4861 tttggcgtat agcccgcaaa taggatatct ggatacaccc cagaagaaat accaagtttt
     4921 gcatactcgt catctgtgta attggatgtt ccggtcttac cggcttgata taacccagcg
     4981 atttgcgcat tcgtaccagt tccttcagtg atcgtatctt ttaaaatgtc tgtgatcata
     5041 taagctgttt ctggagacat ggctgttttt ccatctggct cataagtttc ttctgttccg
     5101 tcttggaaga cgattttatt aacatattga ggtttgtaat aagtccctcc attggcgaaa
     5161 gccgcatagg ctgctgccat ttttaaggaa gaagctccgt attttgtccc atcttgttct
     5221 tccgtattac tagagatcgc attcgattga tggatcgtac tgtattcgat cccaagattt
     5281 tttaagaaag aagccacttt atctgaaccg acttcattga ataatttgac tgctggaaca
     5341 ttacgggata agtaaagtgc ttggcgtaaa gtaatggtgc ccatatattg attatcccag
     5401 tttcctacag gagtcgaagt cccttcatag ttatacggtg cgtcagtaat agtttttcca
     5461 gtagaatatt tcaaatattc aaaagctggt ccgtaatctg tgacaggttt catcgtagaa
     5521 ccaaaatcac gggaagtatt cactgctaag ttgttgccta aagttacgtc ttctgcgatg
     5581 tgacgtccgc caatctgggc ttttactttc ccggtattgg tgtcgataag ggtggaggca
     5641 acttgcatct cgtcgtctgg gtaagagaca tactgatccg tgttgacgat atcatatagt
     5701 ttcttttggg cgtccagatc caaattcgta taaatctcta aaccgtctgt gtagacattc
     5761 ttatctgttt tttcttgtac ttcattgatg acttctttga cataattatc gacgatctta
     5821 gtattgtcat cggattgagt tagttcttgc agaccatccg ttaccggtac attaacagct
     5881 tgatcatatt ctgtttgaga gattttttcg ttttgaagca tcgtatacaa aacagtatct
     5941 cgtcttttct tcgcctgatc tggataaaca taaggatcat aggcggaagg ggcttgaggc
     6001 atcccagcaa gcaatgctgt ttgcggtaaa gaaagttctg ataatttctt accaaagtac
     6061 atctcagaag ctgtctccat cccgtacaag ccgtttgaca tatacacttt atttacgtag
     6121 taggtcaaga tctcttgttt cgattttttt tgttccaatc ttacagccat ccacgcttct
     6181 tgggccttgc gtttcagagt ctgatcctcg gcactggtgg agaagaagga aagtttgatc
     6241 aattgctgtg tcaaagtact tccgccttgc agtccgccag aggtaaagtt agagagggca
     6301 gaaccgatga tccgaatagg gtcaaccccg atgtgtttat aaaaacggcg atcttctacc
     6361 gacacgatcg catcttctaa tgttttcggt aattcattcg cagaaatttt ttcacgcttt
     6421 tctgcgccta aatcttcgaa tagttcgcca tcttgtgtat atagtttgga agatacggta
     6481 gcgtctaatt ttttatccgt caattcgggc gcatcttttg cgtaatacca gaaaagtcct
     6541 acacctgcta aaaagaggat acagaaaaat gagagtattc ctaaaaggat cttgataaat
     6601 aatccttttt tatgtgttcc agaggacttt ccagaacctt tgccagaatt cttcttcaca
     6661 ctctttttag gagtaggctg tttctgtcgt ctagagcttc tagtttgttc atttgccatt
     6721 cttgctttct ccttcaatag ataatgttga taaatagaag tcaaccgcgt ccaaataagg
     6781 aatccgcgga gaaataccag ttttgatctc tattcctagt tcttcgatca aagataaagg
     6841 tagtgatttg cgtccttttt ccttttgggc gtcccagtaa gcaatcaaat gaacactatt
     6901 caaataaaaa cagcgcttca aagacgaaaa ccataacaag acaaagcaga ccccattttg
     6961 ttcgaggcat gctttcatat gttcgatttg atgggcatgg aagtttttaa agggaaaaga
     7021 tgttttgttc ttggtttctt ttgcttcaaa gtccaagtaa aagcctcgat agacgccatt
     7081 atagtctgta gttgaggctt gggtaaagta ggcttccttg atcacagcag cacttctctt
     7141 aggatagtct actttcacga tttggatagg ggtaggcttt ttatggataa ccgccaaccc
     7201 gcgggaaaga taatatttat tactttcatt gattgcttct tcaaagcgca tcccccgatt
     7261 gccgaattca gtgatctgat ttcttttttc ttgaattttt tcgacgggag aggcattctt
     7321 ataataaggt tggccatttg gatagcgcaa taccatcttt aatcacactc ctaggccaca
     7381 ttatagcaaa aaagcggata gaaagaaaag gaatctgaga tggataagtc aaaagtaatc
     7441 tatgtgtctg ggtatcgaag tttcgaactc ggtgttttta aagaaaatga tcctaaaatc
     7501 caagttgtta aaaatgtttt aaaaaaagaa ctccatcagt tagcagaaga agggctggaa
     7561 tgggtacttg tcagtgggaa tttgggcgtc gaactatggg ctgtgcaaat agtagcggaa
     7621 ttgaaagaag aatatccgga actatctctt ggtattttgt atcctttttc tgattttggt
     7681 aataactgga atgaagggaa ccaagaaaaa aaacaattag cagaacaatt agcagattat
     7741 gtcgaagctg ttagccatca accctaccaa tcacctagtc agctaaagaa tcacactcgt
     7801 tttatacttg agcatacagt gggctgtctg ctgatctatg atgaagaatt ccctggaaaa
     7861 acgaagtatt tttttgaaga tgcacagaat taccaagcag atcatcccta cgaaattcgg
     7921 ctgatcagta tggacgatct tcaaaatttt agtgaataat cgatattgtg tttgatttat
     7981 caactttttt cctatacaat gaaaaaggta gaaatgttat tacgtaaatg aaaactagag
     8041 gtgtaaatat ggcgaatttg gtttatagtc ctaaagacat tttacaacaa gaattcaaaa
     8101 caaaaatgcg cggctatgat cccgttgaag ttgatgaatt cttagacaat atcatcaaag
     8161 attatgaaac ttacagcaaa gaactccttg ctttacaaga agaaaacgat cgattgagtg
     8221 caaaagtagc tcaattatct aaaactcaag gagcagctca aacacgcgtg caacaaacag
     8281 aggcaccaaa gagcgcagca gtgaccaatt ttgatatctt aaaacgcttg tcaaatcttg
     8341 aacgtgaagt atttggtaaa aagcttgatc aacaagcaag tgcagtcaaa ccagcacaac
     8401 ctaatccaaa taactacaca aatgcagaca caagcttaga tgataatgaa aaaacacgtc
     8461 aattttaatt tttgattaat gaatttgcgg ttttcgggta atcgcggtct gacttgatca
     8521 ggctgaggaa agtccatgct cgcacaggct gtgatgcctg tagtgttcgt gcttagcgaa
     8581 accataagct aaggtactct ttggagtaac ggcaggaaaa caagctaagg cttcggctat
     8641 gcttaagtat ccttgaaagt gccacagtga cgaagcggct ggggaaaccc atccggtgga
     8701 acgcggtaaa cccctcgagc gagcaaccca aacaatggta ggggcgcttt ttgtctggaa
     8761 gaagaacagg acaaaaaggc agtataactg cagatagatg gttaccgctt tgttttttct
     8821 ccctggaaaa gcaaagtaca gaacatggct tatagaaaac cgcaaacatc agggaattag
     8881 gtcgtgacaa aagtaatgcg ttataagaaa caagaaaaac atttgaaagc tatttttcaa
     8941 tacttcagat gaatcgttct tattacaaaa caaacttttg tatcaccggt tattcggaat
     9001 ttgagtaaaa gtgtggggta ggactgatgt cccacctcct ttttttataa ataaggagat
     9061 tgtgtatgga aaaaaacagg aagttcaacc tcgtcgcaac tgcggcaagc gggctagaag
     9121 cactggttgg taaagaatta agagatcttg gaatcgaatg ccaagttgag aacggacgtg
     9181 cacgttttga aggaacaatg gaaacgatcg ctacagctaa tttatggtta cgtacagcag
     9241 atcgagtaaa aatcgtggtt ggggaattcg atgcgtatac gtttgatgaa ttgtttgaaa
     9301 acgtaaaagc attgccttgg gaagattttc ttccactaga tgcagctttt cctgtagctg
     9361 gaaaatcgat caagtcgaaa ctatatagca cacctgattg ccaagcgatc acaaaaaaag
     9421 caatcgttga acgtctccgt acctattatc accgtccagc ttctgtacca ctgacggaaa
     9481 ctggcgcaca ttttcagttg gaagtagctt tgctgaaaga ccatgtgatg gtaactctgg
     9541 atacgacagg accaagttta ttcaaacgtg gttatcgctt agaaaaaggt ggagcaccat
     9601 tgaaagaaaa catggcagca gcacttatta tgttgacaaa ttggcgaaaa gaccgtccat
     9661 tttatgatcc tgtatgtggt tcaggtacgc tttgtattga agcagcctta ataggccata
     9721 atatcgcacc aggattcaat cgtgagtttg cttgcgaatc ttgggattgg ttcagtcaag
     9781 aaatcatgga aaatgttcga tcagcagccg aagaaaaagc agattatgat attgaattag
     9841 atattactgg ttcagatatc aatggacgga tgattgaaat tgccaaagcc aatgcagaag
     9901 aaatcggatt aggctcttcg attaccttta agcaacaagc agtcaaagac ttcaaaacgg
     9961 ataaagaata cggggtcatc gtggctaatc cgccttacgg agaacgtcta ggagaagaag
    10021 aaactgttcg gaaattatat aaagaaatgg gcgatgtatt ccgtccatta aaaacatgga
    10081 gtaaatatat cttgactagc gacttagcgt ttgaggaatt ttacggacaa aaagcaacca
    10141 aaaaaagaaa attgtacaat ggtgcattgc gtacggatct attccagtat tggggaactc
    10201 gtccgcctag aaaaaaagag aaagacgaat aatcttacta attgattcat tcaattggat
    10261 gtttggagga atagggaatg aaagaaaaag agtttttaca agaagtaaaa gaaatcaatc
    10321 ttctacaaca agctttagga atcctcgatt gggatacaca aacaggtatg cctgaaaaag
    10381 ctagtgcata ccgaagtgaa gtagacagct atctttacag catttatttt tcaaaaaaaa
    10441 tcggtccaaa aatcaaagaa gccattcgct atttttctga acatccggaa gaattgtcgg
    10501 aagttggaac tgccgttttt gccaaagtaa aagaagagta tgatttggaa catcgcgtac
    10561 cggaagatct tatgagaaaa tatacagctg ccgtttcttc agcacacgga caatggcaaa
    10621 aagctagaga aacacaacaa ttttctgatt ttcaacacgc attgaatcaa aatattgaat
    10681 taacaaaaca gttgatacct tactggagaa aagatgaaaa gacgaattat gatgttttgc
    10741 tgaatcaata cgaacccggg atgactgtcg aaatccttga taaggtattt gatcagctta
    10801 aagaaggaat tctttcgatc cgccgtacgc tccaagaaaa agggactgaa cctgaaacag
    10861 actttttaaa tcggagaatg acgaaaaaac aacagaagag atttgtagtg aaagtaatcg
    10921 aacaacttgg ttatgatttt agtcgtggtc gtttagatga cactgtccac ccatttatga
    10981 ctggaattaa tccgaatgat gcacgaatca cgacacgatg gaacgaaaca gatttcaaaa
    11041 tggctgtttt tggagtgatc cacgaggctg gtcatggtat gtatgaacaa gacatcgatg
    11101 ataaatatgc gtatacaccg gtttatgaag gtacatcaat gggtatccat gaatcacaat
    11161 cgttgtttaa cgagatcatt attggaagta gtcgcagctt ctggaaaaag caatatcctt
    11221 tctttcaaga atgtgcagaa ggaacattcg atgatattcc ttttgacaaa ttttatcgtt
    11281 cattgaacca tacaaaatca agtttggttc gtatcgaggc agacagtttg acttaccctc
    11341 tccatatcat cattcgttat gaaatcgaaa aaatgatttt caatgaaggg gtagatgttg
    11401 ctgaactacc tgaaatatgg aatcaaaaat atgaagagta tctgggaata cgaccaacaa
    11461 atgatgtgga aggcatccta caagacgttc attggtcagg tgcaagcttt ggttattttc
    11521 cctcttatgc attagggtac atgtatgcgg cacaattact tcattctttg cagcaagaaa
    11581 tagatgtgga aaatgttcta gcgtcagacg attattctcc aatcaaaaac tggatgtcag
    11641 agcatatcca tcaatatggt gcatctagaa aaccaaatca attaatcatg gatgcaacac
    11701 atgaaccgct taatccgcaa tacctgatcg actatatgaa agacctgtat tttaatgtat
    11761 atcaggtaaa ataaaataca atttacaaag atagctgaaa ttcatctaat ctatagatgt
    11821 tttcagctat ttacttaaga aagaagtgga acgaatggat aaattacaag tgatcgctga
    11881 ttatagtatg gaccaattag cgcatgatca aacaggacat gggagcgatc ataccaaaag
    11941 agttgtcaag ttagctgaaa aaattttgga gacggaaccg caggcagacc gctttgtgac
    12001 gcttgcagca gcttacctac atgatacgat tgatgataaa gtcgttaaaa atgagaacga
    12061 ggcaaagcaa cagcttcgtg tattccttcg ctctttgcca atagtagagg aacagatctc
    12121 gatgattttt tccattatcg aaaatatgtc cttttctaaa aatctctctg aagcggccga
    12181 actatctctg gaaggtaaga tcgttcaaga cgctgatcgt atcgaagcat taggagcaat
    12241 cggcattttg cgtactgctt atttcggcgg tggacatggg cacccgattt ttgatcctga
    12301 actgtatccg caaacattca aggacaaaga aaattaccga aaaggcacga cagtgatcaa
    12361 tcatttttat gaaaaactgt tcttgctgtc tgataaaatg aatactgatt acggatatga
    12421 agaagccaaa cgaagagaaa ccttcatgcg tgaattctta gaagaatttt ttctggaatg
    12481 gtctattgac gatgaaaatt ttactccaga caattggatc aaaaataaca aaaaatgatg
    12541 gaggttgtga cagaagtggt cagctccaag atatttcgga tcattttccg taaaagttac
    12601 ttctggaaca ctgtttacca agaggtcgtg agcctgccgt tcagctccga gtcacaagga
    12661 acaatttcaa aaaatagctt ctcgtgtttg atgaatattg taacttgtga ttcggaattg
    12721 gcaggagaac accgtttatt
//
LOCUS       DASHHB010000071        12734 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.074, whole genome shotgun sequence.
ACCESSION   DASHHB010000071 DASHHB010000000
VERSION     DASHHB010000071.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12734)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12734)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12734
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.074"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(85..264)
                     /gene="rpmF"
                     /locus_tag="U2K71_002396"
     CDS             complement(85..264)
                     /gene="rpmF"
                     /locus_tag="U2K71_002396"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HEM7316422.1"
                     /translation="MAVPARRTSKAKKAKRRTHYKLTIKGLNECPNCGEMKKSHHVCA
                     NCGYYDGKDVMSKEA"
     gene            complement(322..891)
                     /locus_tag="U2K71_002397"
     CDS             complement(322..891)
                     /locus_tag="U2K71_002397"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HEM7316423.1"
                     /translation="MKWSLLELRKYQETPLTFHETLDLKAALMKRDNLILDVAPVEVE
                     GLVSVDKKGYIVHYTVQTTLTVPSTRSLEPVELPMDFSVDEVFMTEEQFQSRDEQMSA
                     DEILLIEGPLLNLAESIEDNILLAIPMRVLTKEEEAATELPKGNDWEVVSEEEYQRRK
                     MEEAENKIDPRLAKLSELFDSKSKDDDNK"
     gene            1077..1979
                     /locus_tag="U2K71_002398"
     CDS             1077..1979
                     /locus_tag="U2K71_002398"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM7316424.1"
                     /translation="MNTKDIEYYVQIVKQKNFTKVANYFRVSQPTITYALQRLEKEFD
                     TVLITRDRSHHELIVTPSGKQLFLHAQTILQEISTARAEITQLQKKKLRFGMPPIIGN
                     EYFPKLSSYFIKHELMNQIEITDGGSRDLYGMIRQGRIDLAVLGSTQPIVDQYLETEL
                     LLDKRFMIVVPPTHPLSKQDTVSFKDIADEQFVLLNEHYVHPAAFTKLSKQTHTHPKV
                     VYQSNDLTILKSMVRENVGIGFLAEIAVHPEDHLVCLPLSDEVQPRFLISLAKRTQQI
                     VTPLQEQVVQVIHEFVTAQKNSSS"
     gene            complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="U2K71_002399"
     CDS             complement(2014..3798)
                     /gene="pyk"
                     /locus_tag="U2K71_002399"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HEM7316425.1"
                     /translation="MKKTKIVSTLGPASNSVEIISQLIESGANVFRFNFSHGDHEEQL
                     SRMTMVREAVKKTGKDVGILLDTKGAEIRTTVQGTTEADFGRAGYIKFEVGDQTRISM
                     DPEHVGSKEKIAVTYPGLFDDVHVGGHILFDDGLIDMQIIEKDEANRELVVEVKNAGM
                     LGSRKGVNAPGVSISLPGITPKDADDIRFGLDNDIDFIAASFVRKAQDVLDIREILEE
                     KDMTHVQIFPKIESQEGIDNIDEIIKVSDGVMIARGDMGVEIPAELVPMVQKRIIKKC
                     NAAGIPVITATQMLESMQENPRPTRAEASDVANAVFDGTDATMLSGESANGDYPVEAV
                     STMARIDQEAENALSELGTFQLNEFDKTDVTETIGLSVARAAKNLGVKTIVAATESGY
                     TAKMISKYRPDADILAVTFDERTKRGLMLNWGVYPTVAEKPSTTDEMFELAAKKAVEL
                     GFASEGDLILITAGVPVGERGTTNVMKIQMIGSKLLEAQGVGQKSVVANAVIASSAEE
                     AVKKAKDGMVLVVPSTDKEYMPAIEKAAAVIVEEGGLTSHAAVVGIAQDIPVIVGAKG
                     ATTTITEGELITVDSRRGIVYRGETTAI"
     gene            complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="U2K71_002400"
     CDS             complement(3872..4834)
                     /gene="pfkA"
                     /locus_tag="U2K71_002400"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010746303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HEM7316426.1"
                     /translation="MKRIGILTSGGDAPGMNAAVRAVVRKGIYEGLEVYGINYGFAGL
                     VAGDIRRLDVADVGDKIQRGGTFLYSARYPEFATEEGQLKGIEQLKKFGIEGLVVIGG
                     DGSYHGAMALTKRGFPAVGIPGTIDNDIPGTDFTIGFDTAINTVLESIDRIRDTATSH
                     VRTFVIEVMGRNAGDIALWSGVAGGADEIIIPEHDFDMASVAKKIQEGRDRGKKHCLI
                     ILAEGVMGGNEFAEKLSEFGDYHTRVSILGHVVRGGAPSARDRVLASKFGAYAVELLQ
                     EGKGGLCVGIHDNEIVASDIIDTLENNKHKPDLSLYDLNNSLSF"
     gene            complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="U2K71_002401"
     CDS             complement(5002..8316)
                     /gene="dnaE"
                     /locus_tag="U2K71_002401"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HEM7316427.1"
                     /translation="MFFPQLYTITSYSLLQSTIRIREYVQEAKKQGYTSLAITDKDVL
                     YGAPEFYRICVEEGVQPIIGLHLSYTYEEKNYGLLAYAKDEIGYQQLMKLSSRKMIQE
                     KLELAEMTETDHLAFVVPANHSVSEAFNKEDNFFGHWQFLLENVFTRNFYIGLTENTP
                     DQLPEWYQYVIKENYSLCALHPIASLQQEELFALEVMKHIKEGSQIPLENLSASLPLT
                     ANVYLLSEQEMIKRFEKAGLSQAVIGAQRLAESISFEFQFHQKLLPHYPVPNGKQAGE
                     YLRELCYSQVERRITDYTKEYQERLDYELSVIHQMGFDDYFLIVWDVMAFAHRNKIVT
                     GAGRGSAAGSLVAYVLEITDVDPLEYDLLFERFLNPERFTMPDIDLDIPDNRREEVLN
                     YVREKYGQYHMAQIATFGTMAAKMVLRDAARVFGLSQSEANRWSKAIPNQLKITLTQA
                     YKTSHQLVELVQASEKNQLLFKTAVILEGLPRHVSTHAAGVVISDKNLLELVPLQRGS
                     DEAFLTQYTMNEVEAIGLLKMDFLGLRNLSIIDFTLKAVKRVEGYEIRLKEIPLNDAK
                     TLLLFQRGETSGVFQFESAGIRNVLRRLDPENIEDIAAVNALYRPGPMQNIDTFIARK
                     KGKEAIRYPDDSLIPILKNTYGVIVYQEQIMQIASTMAGFTLGQSDILRRAVSKKKKD
                     VLDEEKSHFVSGALQQGYSEKTAATVYEYIEKFANYGFNRSHAFAYSFVGFQMAYLKA
                     HHPGAFFTSLMNSVRHNTSKLKEYIAEARKNKLKLSSPSINQSNSSFELVNKEEIRFG
                     LTAIKGIRRDFVEDILNERKQNGPFQSVDQFLIRIDKRWLKLELLQSLTAVGVFDELS
                     PNRKQLMLDLEGKIQNIVYSGGSLDLLGIMALKEEEVADYTLEERLKLEEEYLGIYLS
                     GHPAEGFDRLRLAKQVQLITDIVPNQPVNLLVMIKEVREIRTKKGELMAFVEGTDSSG
                     EISVTVFPTLYRKYRNLFAVDRVIFVQGRSEISKYNQELQLIGESFADPNQLEAQYPS
                     QTCYLRITEEREDPEIMKQLQVIFQKHPGYIPVVMYHEKTQRKVVLSDAYWVDDSKSI
                     KGQLIYLLGEDNVVFK"
     gene            8547..8732
                     /locus_tag="U2K71_002402"
     CDS             8547..8732
                     /locus_tag="U2K71_002402"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjzD family protein"
                     /protein_id="HEM7316428.1"
                     /translation="MRYIVTLFWAVVLGQVVGYIGAALTSGTYDFTLTTIISFIAGVI
                     ILLIGAVAPRKETSAHS"
     gene            complement(8794..9990)
                     /locus_tag="U2K71_002403"
     CDS             complement(8794..9990)
                     /locus_tag="U2K71_002403"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HEM7316429.1"
                     /translation="MEINWRKNLVIAWIGCFFTGASISLVMPFIPVYVEQLGTPKDQI
                     ELFSGLAISVTAFASAVVAPIWGNLADRKGRKLMMIRAAAGMTLTMGSLAFVPNAYWL
                     LIMRFWNGILSGYIPNATAMIASQAPREKSGWALGTLSTGAIAGNLIGPSMGGALAQW
                     FGMENVFLITGALLMITTVLTIFLVKEDFHPIEKKDLISTKEIFSKMDHLSILIGLFI
                     TTLILQIGITTISPILTLYIRELSGSTENILFVSGLIVSIAGVSAVFSSPKLGKLGDK
                     IGNQKVLIGGLILSFCCYLPMAFVTTPLQLGILRFILGFSTGALMPSVNTLISKITPQ
                     EGVSRIYSYNQMFSNFGQVLGPMLGSTVAHAYNYSAVFIVTSLFVLSNILLSLFNFRK
                     VLRSRL"
     gene            complement(10160..11596)
                     /locus_tag="U2K71_002404"
     CDS             complement(10160..11596)
                     /locus_tag="U2K71_002404"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NCS2 family permease"
                     /protein_id="HEM7316430.1"
                     /translation="MEKFFGLKKNNTNVSTEIMAGVTTFFAMSYILFVNPTILSASGM
                     PFQAVFLATIIASIIGTLVMGLFANVPYAQAPGMGLNAFFTFTVVFGLGYSWQQALAM
                     VFICGLINIFITVTNIRKMIIRAIPESLQHAIGGGIGIFVAYVGIKNAGFLSFSADQS
                     AISSSVVEGGKATNVTINGGIVPALANFDNAPILLAVIGLVLTAILVVKNVRGAILIG
                     IVATTVLGIFMGVVDLSSIDWQTNSLGNSFKELGTTFGAAFGSEGMQSLFSDSSKIPQ
                     VLMTIIAFSLSDTFDTIGTFIGTGRRTGIFSKEDEIALEDGRGFKTKMDKALFADAIA
                     TSVGAIFGTSNTTTYVESAAGIGAGGRTGLTSVVVAVLFALSSLFSPLIAIVPNQATA
                     PALILVGVMMMASFADIKWLDMEEALPAFFASIFMGLCYSISYGIAAGFIFYTIVKVI
                     KGKYNQVSVALWIVDILFIINFIILATI"
     gene            11889..12698
                     /locus_tag="U2K71_002405"
     CDS             11889..12698
                     /locus_tag="U2K71_002405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM7316431.1"
                     /translation="MNKKLIAIDLDGTTLNQDSMITSKTKETLKKAINAGHHVSIATG
                     RPFRMSHQFYQQLELTTPMVNFNGALVHIPNQHWDGERETLINREIAFEILSQKKQLN
                     LDFIAAENRDTFFIDSFDFFDEKIFASSRPGEKNLLSPKNLTTNPTSLLVRTDKRFAG
                     AVSAELTRQFGSYVDVRTWGGPTAILEIVSKGIQKAKGVQEIANYLSIDQKDVIAFGD
                     EHNDLELLDYAGWGVAMANGTDQLKGIANDVTPLSNQEDGLAVYLEKLLKL"
BASE COUNT         3802 a         2673 c         2250 g         4009 t
ORIGIN      
        1 attttttatt taccgactac aaaaaaataa acctcaaggc tccatggtta gccttgaggt
       61 tttacatttt ctaattaaaa tgtattaagc ttcttttgac attacgtcct taccatcata
      121 gtaaccacaa tttgcacata cgtgatggct ttttttcatt tcaccacagt ttggacattc
      181 gtttaaccct ttgattgtca atttgtagtg tgtacgacgt tttgcttttt tagcttttga
      241 tgttcttcta gctggtactg ccattttgct acacctcctt gtaaagtctt gtatctaaat
      301 tagacacaat atattccaat cttacttgtt atcgtcgtct tttgatttag aatcaaaaag
      361 ttcagatagt tttgcaagac gaggatcaat tttattctcg gcttcttcca tttttctgcg
      421 ctggtattct tcttcggaaa caacttccca atcattgcct tttggaagtt ctgttgctgc
      481 ttcctcttct tttgtcaata cccgcattgg aatcgccaat aagatgttgt cttcaatcga
      541 ctctgctaaa ttcaataatg gaccttcaat tagcaaaatc tcatctgcag acatttgttc
      601 atctctactt tggaattgtt cttctgtcat gaatacttcg tctacagaaa aatccatggg
      661 cagttcaaca ggttctagtg aacgtgttga aggaactgtc aaagtcgtct gcacagtgta
      721 atgaacgata tagcctttct tatcaactga gaccaaacct tcgacttcca ctggcgcaac
      781 atcaagtatt aaattgtctc gcttcatgag agcggctttc aagtcgagcg tctcatgaaa
      841 agttaacggt gtctcctggt attttctcaa ttccaataaa gaccatttca tttttcatca
      901 ctcctaagca acaaaagcaa ttatacaggg caaaaagcat gttgtcaatg aaattttctt
      961 gacaccttgc agttcataaa aaaacagaaa aatctttatt agaaagcctc tgtttgctat
     1021 catcctcaga atcatttaca ataatcatta ttccattaaa ggagcggatg atcttcatga
     1081 acacgaaaga tatcgaatac tatgtccaaa tcgtcaaaca aaaaaatttt acgaaagtgg
     1141 ctaattattt ccgtgtgagc caacctacta ttacatatgc attacaacgt cttgaaaaag
     1201 agtttgacac cgtacttatt acaagagatc gatcgcatca cgaattaatc gtcacgcctt
     1261 ctggaaagca gctgtttctc catgcacaaa ccattctcca agagatttct actgcaagag
     1321 ctgaaatcac tcagttgcaa aagaaaaagc ttcgttttgg catgcctcct attatcggca
     1381 atgaatactt tcctaaactg tcaagttact ttatcaagca tgaattgatg aatcaaattg
     1441 agattaccga tggaggctct cgggacttat acgggatgat ccgacaaggg aggatcgatt
     1501 tagctgtatt aggctcaaca cagccaatcg tggatcaata tttagaaaca gagttacttt
     1561 tggacaaacg tttcatgatc gtcgtgccac ctacacatcc tctttccaag caggatactg
     1621 tctctttcaa agatatagca gatgaacagt ttgtactatt gaacgaacat tatgtccacc
     1681 ccgcagcatt cactaagctt tctaaacaaa cacatactca tccaaaagtt gtttatcaaa
     1741 gcaatgactt gacgatttta aagagtatgg tccgagaaaa tgtgggtatc ggttttttgg
     1801 cagagattgc tgtccatcct gaagatcatt tagtttgcct ccctctttca gatgaagttc
     1861 agcctcgctt cttgatttca ttggcaaaac gaacacagca gatcgtcacg cctcttcaag
     1921 aacaagtagt ccaagttatc catgaattcg ttacagcaca aaaaaacagc tcttcgtaaa
     1981 agaagagctg tttttatttt ttattaagct aaattagata gctgttgttt cgccacggta
     2041 aacgatgccg cgacgtgaat caacggtaat caattcacct tctgtaattg ttgttgttgc
     2101 gcctttagca ccaacgatta caggaatatc ttgtgcaatt cctacaactg cagcgtgaga
     2161 agtcaatccg ccttcttcaa cgataacagc agctgctttt tcgattgcag gcatatattc
     2221 tttgtcagtt gatggtacaa ctagaaccat tccgtctttt gcttttttta cagcttcttc
     2281 agcagatgaa gcaataactg catttgcaac gacagatttt tgtccaacgc cttgagcttc
     2341 taataattta gaaccaatca tttgaatttt catcacgtta gttgttccgc gttcaccaac
     2401 tggtacacca gcagtgatca agattaaatc gccttctgat gcgaaaccta attcaacagc
     2461 ttttttagct gctagttcaa acatttcatc agttgttgat ggtttttcag caactgttgg
     2521 gtaaacgccc cagtttaaca tcaaaccacg ttttgtgcgt tcgtcaaaag taacagccaa
     2581 gatatctgca tctggacgat attttgaaat catttttgca gtgtatcctg attcagttgc
     2641 agcaacgatt gttttcacgc ctagattttt tgcagcacga gctacagata aaccgattgt
     2701 ttcagttaca tctgttttat cgaattcgtt caattggaat gtacctaatt ctgataatgc
     2761 attttcagct tcttggtcga tacgtgccat tgttgaaaca gcttcaactg ggtagtcccc
     2821 gtttgcagat tcaccagata gcattgttgc atctgtgcca tcaaatacag cgttagcaac
     2881 gtcagaagct tccgcacgtg ttggacgtgg gttttcttgc attgattcta acatttgtgt
     2941 agcagtgata actgggatac cagcagcatt acattttttg ataatgcgtt tttgaaccat
     3001 tggtactagt tcagctggga tttcaacacc catatctccg cgggcaatca taacaccgtc
     3061 agaaactttg atgatttcgt caatgttgtc gataccttct tgagattcga ttttagggaa
     3121 gatttgaaca tgagtcatgt ctttttcttc taagatttcg cggatatcta gaacgtcttg
     3181 tgctttacgt acaaaacttg cagcgataaa gtcgatatcg ttgtctaaac caaaacgaat
     3241 atcgtcagca tcttttggag taatacctgg caaactaata gaaacgcctg gtgcatttac
     3301 ccctttacgt gatcctagca tacctgcatt tttaacttct acgactaatt cacggtttgc
     3361 ttcgtctttt tcaatgattt gcatatcgat caaaccatca tcaaataaaa tgtgtccgcc
     3421 aacgtgaaca tcgtcaaata atcctgggta agtaaccgcg attttttctt ttgaacctac
     3481 atgttcagga tccatagaga tgcgtgtttg atctcctact tcaaatttga tgtatcctgc
     3541 acgaccaaaa tcagcttctg ttgtgccttg aacagttgta cggatttcag ctccttttgt
     3601 atctaaaaga ataccgacat cttttcctgt ctttttaact gcttcacgga ccatagtcat
     3661 acgtgataat tgttcttcat ggtcaccatg agaaaaatta aagcggaaga catttgcccc
     3721 agattcaatc aattgagaaa taatttctac tgagttactt gctggtccta atgtactaac
     3781 gattttggtt tttttcattt aaaaaactct cccattcaat tttgtttatt taaagctgta
     3841 cacagcttaa atagcttggt ttctttcact cttaaaatga aagtgaatta tttagatcat
     3901 atagtgataa gtctggttta tgtttgttgt tttcaagtgt atcaatgatg tcagacgcaa
     3961 cgatttcgtt gtcatggata ccgacacata atccaccttt tccttcttga agcaattcga
     4021 cagcgtaagc accgaattta cttgccaaca cgcggtcgcg tgcgcttggt gctccaccac
     4081 ggacaacgtg tcccaagata gatacacgtg tatgatagtc accaaattct gaaagtttct
     4141 cagcgaattc atttccgccc attacacctt cagccaagat gatcaagcaa tgtttcttcc
     4201 cgcgatcgcg gccttcttgg atttttttag caactgaagc catatcaaaa tcatgttctg
     4261 ggatgatgat ttcatccgct ccgccagcca cacctgacca taaagcgatg tctcctgcat
     4321 tgcgtcccat tacttcgata acgaatgtac gaacatgaga agtcgctgtg tcacgaatac
     4381 ggtcaattga ttctagtact gtattgattg ctgtatcaaa accgattgtg aaatcagtcc
     4441 caggaatatc attatcgatt gttccaggaa tacctactgc tgggaaaccg cgttttgtca
     4501 aagccattgc gccatgataa gagccgtctc ctccgataac cactagacct tcgattccga
     4561 attttttcaa ttgttcgatt ccttttaatt gaccttcttc agttgcaaat tctggataac
     4621 gtgcagaata caagaatgta ccaccacgtt ggatcttgtc acctacatct gccacatcaa
     4681 gacgacgaat atctccagca actaaaccgg caaaaccata attgatcccg tatacttcca
     4741 gtccttcgta aataccttta cgaacaacgg cacgaacagc tgcattcatt ccaggtgcgt
     4801 ctccgccact ggtcaaaatt ccgatgcgtt tcataataaa cattcacctc attaaaatac
     4861 tttatcctaa gtatgactta ggtttacaac catttttgcg ttaaccacga ctaacattct
     4921 aacattattt ttctcacttg tcttgattta cggataaaaa aatgaaaaaa aatgaaagca
     4981 agtagtattt tttaccgtaa gttatttgaa aaccacatta tcttcgccaa gcagataaat
     5041 aagttgcccc tttatactct tgctgtcatc cacccaataa gcatctgaca gaactacttt
     5101 tcgttgcgtt ttttcgtgat acataactac tggtatatat cctgggtgtt tctgaaaaat
     5161 cacttgtaat tgtttcatta tctcgggatc ttcccgttct tctgtaatac gcaaatagca
     5221 agtttgtgaa ggatactggg cttctaactg gttaggatca gcaaagcttt ctccaattag
     5281 ttgcagttcc tgattatatt tgctgatttc gctccgccct tgaacaaaga tcactcgatc
     5341 aacggcaaac aaattacgat atttacgata aagagttgga aaaactgtga cagaaatttc
     5401 tccactactg tcggttcctt caacaaaggc catcagttct cctttttttg tccgaatttc
     5461 tctaacttcc ttgatcatca ctaataaatt gactggttga ttagggacga tatctgtaat
     5521 cagctgaact tgtttagcta aacgaagacg atcaaaccct tctgcaggat gacctgacaa
     5581 ataaatacct aagtactcct cttccagctt caagcgttct tctagtgtat agtcagcgac
     5641 ttcttcttct tttaatgcca tgattcccag caagtcaaga cttccaccac tgtaaacgat
     5701 attttggatt tttccttcga gatcaagcat caattgtttc ctgttaggag ataattcatc
     5761 aaaaactcct actgctgtca gtgattgcag taactctaat ttcagccaac gcttatcgat
     5821 ccgtatgagg aattgatcca ctgactggaa cgggccattt tgttttcttt cgtttaatat
     5881 atcttctaca aaatcacgcc gaataccttt tatcgctgtc agtccgaatc tgatttcttc
     5941 cttattgacc aactcaaaac tggaattact ttgattaatg gacggcgagg aaagtttgag
     6001 tttattttta cgtgcttctg caatatattc tttcaatttt gatgtgttat gccgaacaga
     6061 attcatcagg ctggtaaaga aagcacctgg atgatgggct tttagatacg ccatttggaa
     6121 tcccacgaat gaataagcaa atgcatgtga ccgattgaat ccatagtttg caaatttttc
     6181 gatatattca tatacggtcg ctgctgtttt ttccgaatat ccctgttgta atgctcctga
     6241 tacaaaatgc gacttttctt catctaatac gtcttttttc tttttactca ctgcccgtcg
     6301 cagaatatct gactgcccta atgtaaatcc agccatagtc gaggcgatct gcatgatttg
     6361 ttcctgatag acgatcactc catatgtgtt tttcaaaatc gggatcagtg aatcatctgg
     6421 gtagcgaatg gcttcttttc ctttcttcct ggcaatgaag gtatctatat tttgcatagg
     6481 gcctggtcga tacaatgcat tgactgcggc aatatcttcg atgttttctg gatctaatct
     6541 tcgtaaaacg ttccggatac cagcagattc aaattgaaat actcctgagg tttcgcctcg
     6601 ttgaaacaag agcagcgttt ttgcatcatt tagcgggatc tcttttaatc ggatctcata
     6661 cccttctact cgcttaacag ctttcaaagt aaaatcaata atcgataagt tgcgtagtcc
     6721 taaaaagtcc attttcaaaa gaccgattgc ttctacctca ttcatcgtat actgggtcag
     6781 aaaagcttca tcagaaccgc gttggagcgg aactagttca agtagattct tatcgctgat
     6841 aactacacct gctgcgtgag tcgaaacatg tcttggcaat ccttctaaaa tcactgcagt
     6901 tttgaataaa agttggtttt tttcgcttgc ctgaactaat tctactaatt gatgcgaagt
     6961 tttataagct tgggtcaatg tgattttcaa ttgattgggt attgcttttg accagcggtt
     7021 tgcttcactt tgcgataaac caaacactct agctgcatct ctaagaacca tttttgctgc
     7081 cattgtgcca aatgttgcga tttgtgccat atgatattgt ccatactttt ctcgaacata
     7141 attcaatact tcttcacgtc gattgtcagg aatatccaaa tcgatatctg gcatcgtaaa
     7201 gcgttcgggg tttaaaaagc gttcgaacag caaatcatat tctaatggat ccacatctgt
     7261 gatttctaac acataggcta ctaatgaacc cgcagcagaa ccacgacctg caccggtaac
     7321 gattttgttt ctatgagcaa aagccattac atcccatacg atcaagaaat aatcatcgaa
     7381 tcccatttga tggatgacag ataactcgta gtccaaacgt tcttggtatt cctttgtata
     7441 gtcagtgatt cggcgttcca cttgcgaata gcataactct cttaaatatt ctccagcttg
     7501 ttttccatta ggaactggat aatgcggaag taatttctga tggaactgga attcaaatga
     7561 aatcgattca gctaaacgct gggcgcctat tacagcttgg gaaagacccg ctttttcgaa
     7621 tcgtttgatc atttcttgtt ctgaaagaag atagacattt gctgttagtg gtaaagatgc
     7681 agataaattt tctaaaggta tttggcttcc ctctttgata tgtttcatca cttcaagtgc
     7741 aaaaagttct tcttgttgta aactagcgat gggatgtaat gcacaaagcg aataattttc
     7801 ttttatcaca tactgatacc attcaggcaa ttgatcaggc gtgttttctg tcaagccgat
     7861 ataaaaattt ctagtaaaaa cattttcaag taaaaactgc caatgaccga aaaagttatc
     7921 ctctttattg aaagcctcag aaacagaatg atttgccgga acaacaaacg ctagatggtc
     7981 ggtttctgtc atttctgcaa gttccaattt ttcttggatc atttttcggc tggaaagttt
     8041 catcagctgc tgataaccta tttcatcttt tgcgtacgca agcaagccat aatttttttc
     8101 ttcataagta taactaagat gtaatccaat gattggctgt actccttctt ctacacaaat
     8161 acgatagaat tcaggtgcac cgtataagac atctttatcc gttatggcta atgaagtgta
     8221 gccttgtttt tttgcttctt gtacatattc tcgaatacgt atcgtacttt gtaaaagaga
     8281 ataagaagta attgtataaa gttgtgggaa aaacatcttg cattccgcct tttttttcgt
     8341 taaaattcac tagcgttaaa aggtttgatc cttttacttt cattaagata gcaggcgctg
     8401 aagttgatta ttccacttca tttgcttgct tgtaatctgt aggaatgagt tctttcgcaa
     8461 gaactcaaat tttattgaga tttggtctcg tttctttaca ttcaaagaaa atctggtaaa
     8521 attaagatag aaaagaggtg ttctttatgc gatatatcgt tacattattc tgggctgtcg
     8581 ttctaggtca agtagtcggt tatattggtg ctgcattaac aagcggaaca tatgatttta
     8641 ctttgacaac aatcatttca ttcattgcgg gtgtcattat ccttttgatt ggtgcagttg
     8701 cgccaagaaa agaaacctct gcccattcgt aaaaaaacag ccaagagctt gagatttctc
     8761 aagctctttt ttattttcta ctcctcacca ccattataat ctgctgcgaa gaactttacg
     8821 aaagttgaat aaagaaagaa gaatatttga cagaacaaac aaacttgtca caataaaaac
     8881 agcactgtaa ttataagcat gcgcgactgt cgatcctagc atgggaccta aaacttgtcc
     8941 aaagttgcta aacatctgat tgtagctata gattcggctg actccttctt gaggcgtaat
     9001 cttactgatc agtgtattga cagaaggcat caaagcgcca gttgaaaagc ctaaaataaa
     9061 gcgaagtata cctaattgga gcggtgtagt gacgaatgcc ataggtaaat aacagcaaaa
     9121 tgataaaatg agtcctccaa ttagaacttt ttgattccca atcttatccc ctagttttcc
     9181 tagtttcggt gaagaaaaga cagcggaaac accagctatc gaaacaatca agccgctgac
     9241 aaaaaggata ttttctgtac ttccgctcaa ttcacgaata tataaggtca aaatagggct
     9301 gatcgtagtg attcctatct gtaaaatcaa ggtcgtaatg aagagaccga tcaaaataga
     9361 gagatgatcc atttttgaaa aaatttcttt ggtgcttatc agatcttttt tctcgattgg
     9421 atggaaatct tctttgacta agaaaatggt caataccgtt gtgatcatca acaaagcccc
     9481 tgtgatcaag aatacatttt ccataccaaa ccactgagcc agtgcaccgc ccattgaagg
     9541 tccgatcaga tttccagcaa tcgctccggt agacaatgtt cctaacgccc atccgctctt
     9601 ttctctaggt gcctgtgaag caatcatcgc tgtggcattt ggaatatatc cagacaaaat
     9661 cccattccaa aaccgcatga tcaaaagcca atacgcgttt ggtacaaaag ccaaagatcc
     9721 cattgttaat gtcatccctg ctgctgcacg gatcatcatg agttttcggc cttttcgatc
     9781 agctagattt ccccagattg gtgcaaccac ggcagatgca aaagcagtca cagagattgc
     9841 tagtcccgaa aacaattcga tttgatcttt tggtgttcct agttgctcaa cataaactgg
     9901 tatgaatggc ataactaaac taatacttgc accagtgaaa aaacagccaa tccacgcaat
     9961 cactaaattt tttctccaat taatctccat ctatcatccc tctttcgtct cttttatcat
    10021 acctattttc gaataaacaa gcaacgatct cgccagtaaa aagagaaaaa tcccgcaaag
    10081 gtcattttat atgtaacgtt ctttttatgt aaaacaaaaa gagatcagaa caaatactgt
    10141 tccaatccct tctctttagt taaattgttg ctagaatgat gaagttgata atgaataaaa
    10201 tatcaacgat ccacaaagca acagaaactt gattgtattt tcctttgatt actttcacaa
    10261 ttgtatagaa aataaatcct gctgcaatcc cataggaaat gctatagcac aatcccataa
    10321 agatcgatgc aaagaaggcc ggtaaagctt cttccatatc tagccatttg atgtctgcaa
    10381 aagaagccat catcattact ccaactaaaa tcaatgctgg cgcagttgct tgatttggta
    10441 caatggcgat cagtggagag aataaactac ttaatgcgaa tagcactgcc actactactg
    10501 aagtcaaacc agtacgtcca ccggccccga tcccagctgc agattcgaca taagttgttg
    10561 tattcgatgt accaaagata gcccccacag aagtagcgat cgcatcggca aatagtgctt
    10621 tatccatttt tgttttgaac ccacgaccat cttccaacgc gatctcgtct tcttttgaga
    10681 aaatacctgt gcggcgtcct gtaccgataa atgttccgat cgtatcgaaa gtatcagata
    10741 aactgaatgc gatgatcgtc atcaatactt gaggaatctt ggaagaatca ctgaataaag
    10801 attgcatgcc ttcagaacca aatgctgcac cgaaagttgt tcctaattct ttaaatgaat
    10861 tacctaacga attcgtttgc caatcaatgg aacttaaatc aacaacaccc ataaaaatac
    10921 caaggactgt ggtcgctacg ataccgataa gaatagcccc acggacattt tttacaacta
    10981 aaatggctgt cagtaccaaa ccaatcacag ccaataagat aggagcgttg tcaaagttgg
    11041 ctaacgcagg gacgattcca ccatttatcg taacatttgt cgcttttccg ccttcaacga
    11101 cagagctcga aatagctgac tggtctgctg aaaaagataa gaatcctgca tttttgattc
    11161 cgacatacgc aacgaaaata ccgatcccgc caccaatcgc atgttgaaga ctttcgggaa
    11221 tcgcccgaat aatcattttt cgaatatttg tgacagtaat aaaaatattg attaatccgc
    11281 aaataaacac cattgccaag gcttgttgcc aagaataacc aagcccaaaa acgaccgtaa
    11341 aagtgaaaaa tgcgttcaat cccataccag gtgcctgagc atatggtaca tttgcgaaca
    11401 atcccattac cagcgtacca ataatcgatg caatgattgt cgctagaaat actgcttgga
    11461 agggcatacc tgatgctgac aaaatcgtcg gatttacgaa taagatataa ctcatggcaa
    11521 agaatgtcgt tactcccgcc attatttcag ttgatacatt tgtattgttt ttctttaaac
    11581 cgaaaaactt ttccataaaa agttccactc cctgaatttt tttctgatgg aaaactataa
    11641 taataaaaaa taagtaaaat agggcaaatc catcgaacaa tttcaaatta tatagacgtt
    11701 tcattcgctt ttcaacgttt ttttaacaaa aaaagcgaaa ataatactta acgttcgtgt
    11761 ttagcttatg tttagctatt tatatagcca taactgtagt ttttgttagg aatagctgat
    11821 tgctcttgtt aattacctaa aatatgctag actaaaacaa atgctagttg aggaaggaag
    11881 gacccttatt gaataaaaaa ttaattgcca tcgatctgga cggaactacc ctcaatcaag
    11941 attcgatgat tacatctaaa acaaaagaga ctctaaaaaa agcaatcaat gccggtcatc
    12001 atgtcagtat tgctacagga cgcccattcc gaatgagcca tcagttttac caacaattag
    12061 aattaacaac accgatggtt aattttaacg gcgcattagt acacatccct aatcagcact
    12121 gggatggtga aagagaaacg ttgatcaatc gtgaaatcgc ttttgaaatc ttatcgcaaa
    12181 aaaagcagct aaatttagat tttatcgctg ctgaaaatcg tgatactttt tttattgaca
    12241 gttttgattt ctttgatgaa aaaatttttg cttcttctcg tccaggagag aagaatttgc
    12301 tttctcctaa aaatttgacg acaaatccta catctttgct tgtccgaaca gacaaacgtt
    12361 ttgcaggagc tgtatctgct gaattaacac gtcaatttgg cagttatgtc gatgttcgga
    12421 catggggtgg acctactgcc atcttagaga ttgtttcaaa aggaatccaa aaagcaaaag
    12481 gcgtgcaaga gattgctaac tacctgtcta tcgatcaaaa agacgtcatt gcctttggtg
    12541 acgagcataa tgatttggaa ttgttggatt atgcaggttg gggagtagct atggctaacg
    12601 gtactgacca actcaaaggc attgcaaatg atgtcactcc tttgagcaat caggaagacg
    12661 gtttagctgt ttatctggaa aaactactta aactataata gagaattaga ttccacaaaa
    12721 aagaggttgt gaca
//
LOCUS       DASHHB010000072        12621 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.075, whole genome shotgun sequence.
ACCESSION   DASHHB010000072 DASHHB010000000
VERSION     DASHHB010000072.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.075"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            4..2376
                     /locus_tag="U2K71_002406"
     CDS             4..2376
                     /locus_tag="U2K71_002406"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316432.1"
                     /translation="MAEEKEPLDNSRLGKSKRKLVRLQNELNEQIEKMFEHQKMTNGQ
                     PMNDKRNGRSWFNQQERIENKVQSLREEIKQQEKQVEKLERQEELKEMGYNKYGGLDM
                     TIENIPRIREEIERFEKGESSFSAATIRKYQRKLETLEQLKEKSEKGKENILPEVQAI
                     IDSGRVTQWKKNPTIYFLKGYRKVALELNENGEFEESKKYQATSEEEKVAVRELLEEA
                     APTDSLKEKENDHFYRVEFNENDPEKGLKSYGGEIVTLELIDQLKTLDNQYAAEAGYY
                     KFYFEEVKNGEVVDQIRMDLGDGVNSNQPIYLELAKLCEQVPEKEQKQSVKNEPVSKE
                     QMQSKETKTKQKGYQKRTAEEIKQEVKSLSQQALDAVKKHTHSPEDVKELLNFMSRFP
                     ERSFRNQLLIEEQFPGATACLGRAALKKEGIYIKKGEQGNKIFVRKTVKGFYEKGRGF
                     VRETEATPEDKKRIESGQIEVIKKPYYTIEKVFDITQTQLKPEDYPKIFPNRVFDFQL
                     DKKGQSELQAGIQAVANNIGIQIRDMTESEVYQRELGQARGAYVRNEFTGEEEIVMNT
                     RNTPTQHLATSIHELAHARMHKFSELDTATKELQAEMTSYIVCKHFGMDTSEKAIPYI
                     AAWTKNGQTLDDKEAAERGKIMNDVSRVANEFIQTISNEINQYREREPEIQPAEVKQE
                     HKNTLETPENKNIVPVGSLWIDKKDGKVMEEDTGRTLHLKDAAFIEDPQHNQVILKGK
                     SYGKEIQTALPSEEFALGNPFRFSLETVSEVAKRKNEIYYEQQLSQKERV"
     gene            2437..3897
                     /locus_tag="U2K71_002407"
     CDS             2437..3897
                     /locus_tag="U2K71_002407"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014401506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HEM7316433.1"
                     /translation="MTEKKMSLIDRCKQIDIVDFARNNGMAVVNKGRDYRLEDHDSFV
                     FDRRKQRFYWNSQNISGDIIELAKLFFIDKEIQDPKQQFKAATDFILKNEDKTERVEN
                     LHFETEKYKDHPVDYQPLTEKGRNYLKEERKLPEWLIDYAEKEGLIAELKPKYERQNF
                     LVRDDRLDHAVAFLWKDPQTKETVGASYQGTFIDYERFGERGTYKHIDKNSTANHGFN
                     LKIGDPKQIKFFESSIDLLSYAALNRDQLNDTWLVSMEGLKHHVISHYFGEAVSELRK
                     KQAFPQSIEICVDNDRAGHIFYEKEQLMGAVDPFTNQKVRCERGIANDWQVPKEYKII
                     YEEVAKEEKVTPEAIMAIHKTENNLQLTNQLVSAHKVNAFFGQQLSVNDSIEAINLKD
                     ICREVAKELKVCERVDGTYDFDRFYQEKGDINAQILFSYKAEQYYKGYKNHEHEFVPE
                     VKKDWNDQLKHEIQQQEIRKQKRAMLFQQGRQQERE"
     gene            3908..6112
                     /gene="topB"
                     /locus_tag="U2K71_002408"
     CDS             3908..6112
                     /gene="topB"
                     /locus_tag="U2K71_002408"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002369673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase III"
                     /protein_id="HEM7316434.1"
                     /translation="MKTVILAEKPSQAKAYADSFSKATRKDGYFEIQDRLFPGETVIT
                     YGFGHLVELDSPDMYDENWKQWSLEHLPIFPTHYHYHVPKDKKKQFKVVKQQLQSADT
                     IIIATDSDREGELIAWTIIQQVGADQGKTFKRLWINSLEKEAIYQGFQQLRDAKETYP
                     KFEEAQARQIADWLIGMNGSPLYSLLLQQKGIPGSFSLGRVQTPTLYMIYQLQEKIRN
                     FKKEPYFEGKSEVIAQNGAFDAKLDPNETQATQEAFEDYLKEKGVQLGKQPGMIRQVE
                     TEKKSAASPRLFSLSSLQSKMNQLMKASAKDTLEAMQGLYEGKYLSYPRTDTPYITEG
                     EYAYLLDHLDEYKHFLRAEEISTPIHTPNSRYVNNKKVQEHYAIIPTKTVMTEATYES
                     LTPLQQAIYEQVLKTTVAMFAEKYTYEETTILTQVQQLQLKAIGKVPIDLGWKKLFGK
                     ESDGKEKEEEPLLPKVTKGEMVTVDLQVLEKETKPPQPYTEGTLITAMKTAGKTVDSE
                     EAQSILKEVEGIGTEATRANIIETLKQKEYIKVEKNKLVVTNKGVLLCQAVEKEPLLT
                     SAEMTAKWESYLLKIGERKGTQTTFLANIQKFVSHLLEVVPGQIQSIDFGSTLQEVKA
                     ASEKQEAARHLGICPKCQEQEVLLYQKVAACTSEACDFKLWTTIAKKKLTATQLKEII
                     QNSRTSQPVKGLKGQKGSFEATIVLKEDFTTGFEFSEKKKTNFKKRTRRTTK"
     gene            6128..6277
                     /locus_tag="U2K71_002409"
     CDS             6128..6277
                     /locus_tag="U2K71_002409"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316435.1"
                     /translation="MRNTTHKQQEIENQKAKIRSEQEKLRASKRRLQQLKNQQRIQKE
                     IELDR"
     gene            6352..6945
                     /locus_tag="U2K71_002410"
     CDS             6352..6945
                     /locus_tag="U2K71_002410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type IV toxin-antitoxin system AbiEi family
                     antitoxin domain-containing protein"
                     /protein_id="HEM7316436.1"
                     /translation="MQEKLKELLAIRDGNLSMKEARKLGIAATTVQRLVNQGKLIKVD
                     RGYYVEDGHGIDDLFWLQQRFSRGIFSHETTLDIYQLSTNMPKFIHLTFPHGYNVDRS
                     ITKEQQLQFHFVKKEVYELGKVEGTSFQGNPITMYDKERTLCDIWNPRYEIEYEMKLE
                     ALKEYMEDPLRDPSKLRDYMTKLHVEKEMKYHMQSLY"
     gene            6958..7857
                     /locus_tag="U2K71_002411"
     CDS             6958..7857
                     /locus_tag="U2K71_002411"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyl transferase AbiEii/AbiGii toxin
                     family protein"
                     /protein_id="HEM7316437.1"
                     /translation="MTPTQFAAKTRNLAKRTGLNAQLVQRHFMMDRLIEQISESKYKN
                     DFILKGGFLLGSKYGIDRRTTIDIDTTFRNSKLTEEKLKSIFNDLTRKPTKEGIVFSI
                     QGMKETREADFYPGFQIKLVANLEKVRVPFKMDVTTGDSIYPEVDTHYHQLMFEDKKV
                     EIPAYPTAQIIAEKLSATFSFGTDNSRAKDYYDLFTIPKLENIDTKELYKSVRNTFTK
                     RGDTKPLSFYYEREMDKIIGNDYLEKQWEKYRSVNTFANSISFTDTVNEIDHLMKSVI
                     KIENQERKRQFKRSQQMNQEMER"
     gene            7860..8357
                     /gene="ssb"
                     /locus_tag="U2K71_002412"
     CDS             7860..8357
                     /gene="ssb"
                     /locus_tag="U2K71_002412"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000609607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM7316438.1"
                     /translation="MINNVTLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQNGNR
                     EADFINCVIWRKPAETMVTLAKKGSLIGVVGRIQTRTYDNQQGQRVYVTEVVADNFQL
                     LESKAATESRAHADQSSTSPSTTTFEQRDTATPNNNGLNALQNPFGNPFGGQSIDISD
                     DDLPF"
     gene            8723..8983
                     /locus_tag="U2K71_002413"
     CDS             8723..8983
                     /locus_tag="U2K71_002413"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316439.1"
                     /translation="MNKYVGLLDKIRVIKTQPLLVRFTLQTIHESINCVVADIEIIDK
                     LLIMDDGKYNIAVTGHFNKRNQLVIASMYVRNPDHFTRSMGI"
     gene            9428..9634
                     /locus_tag="U2K71_002414"
     CDS             9428..9634
                     /locus_tag="U2K71_002414"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase UvrA"
                     /protein_id="HEM7316440.1"
                     /translation="MRKKFDYWGVPFSELFPNYHAPHTVECDCGQRAKCIKSYRLYQC
                     PTCGKKYTLSYGDYILIDEKGKKR"
     gene            9682..9885
                     /locus_tag="U2K71_002415"
     CDS             9682..9885
                     /locus_tag="U2K71_002415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316441.1"
                     /translation="MVDKEVISEKVAEALLIPRYWTIETPLVEDAPTMLFEDLVKIAG
                     YELHYQENMETYENEYWLTKLEN"
     gene            9901..10338
                     /locus_tag="U2K71_002416"
     CDS             9901..10338
                     /locus_tag="U2K71_002416"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316442.1"
                     /translation="MAQNYYDEFVKLPLDKMAQKMEDMTFLYHETRVPKKHYKERLSV
                     AVEEMIESGVEMNLIATYYRTLEELKKQNAKWFFQALLCLEVGVKPSTIKPSEYQALE
                     LTYGKFVETKKAKTVSSEWLDYFENINKYGAYYTMKKEDNENE"
     gene            10331..11038
                     /locus_tag="U2K71_002417"
     CDS             10331..11038
                     /locus_tag="U2K71_002417"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316443.1"
                     /translation="MNKVIYQLTTEDVSHFLTNKEHLNEVIRRIQKDFSCMEEMKYFI
                     QRVEEQLYLESETSQTKVTVSNNYLDVYKHRPETVKKGTELLRLETEKYIMNVYLERS
                     ELVENFVSCTICRKHYYSESEYYSIFESMELMEEEMEELNSKNATIDDYYLVFTRFLT
                     CGTESIVLMGMDYELAVPEAFLREYVEEVDNPGDDWYTTLTDFLDTYTYDDTEEIQNE
                     LDRKRMPYQRFERKKRQ"
     gene            11418..11597
                     /locus_tag="U2K71_002418"
     CDS             11418..11597
                     /locus_tag="U2K71_002418"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316444.1"
                     /translation="MPIAIGNKRLPVTLDEKRQKELQQLKQKYGKSESKIMCIALDLL
                     IAQEKAGFDIPALKK"
     gene            11922..12185
                     /locus_tag="U2K71_002419"
     CDS             11922..12185
                     /locus_tag="U2K71_002419"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PbsX family transcriptional regulator"
                     /protein_id="HEM7316445.1"
                     /translation="MEMLPIKKWGNSNGLRLPKYIMEYLGIHTEDKVKIIQEEVNGQK
                     RLIIEAANIENELTIEQLFENYTDERNHVTIQNLGEAVGNEKW"
     gene            12179..12529
                     /locus_tag="U2K71_002420"
     CDS             12179..12529
                     /locus_tag="U2K71_002420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016610020.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEM7316446.1"
                     /translation="MVRKPRQGDILLLNTAPRSGHEQTGKRPYIVLSHDIIADYSNVV
                     TVAPISSTTRNYPLYVNINSEYEMKTTGKVLLDQLTTIDYEARECIFLEKAHDSLVEE
                     LLMKVRTVFQKVSK"
BASE COUNT         4732 a         2060 c         2608 g         3221 t
ORIGIN      
        1 gacatggcag aagaaaaaga accactagac aatagtcgtt tgggaaaatc caaaagaaaa
       61 ttagtacgat tgcaaaatga attaaatgaa caaatcgaaa agatgtttga acaccaaaaa
      121 atgacgaacg gtcaaccaat gaacgacaag aggaatggcc gttcatggtt taaccagcaa
      181 gaacgaatcg aaaataaagt ccaaagtctg agagaagaga tcaagcaaca agaaaaacag
      241 gtagaaaaac ttgaaagaca agaagagcta aaggaaatgg gttataacaa gtacggcgga
      301 ctagatatga cgatcgaaaa tattccaaga atcagagaag aaatcgaacg ctttgaaaaa
      361 ggtgaatcgt ctttttcagc cgccacgatc cgaaaatatc aacggaagtt ggaaacatta
      421 gaacagctga aagaaaaatc agaaaaagga aaagaaaata tcctccctga agtacaagcg
      481 atcattgaca gcggacgagt aacgcaatgg aaaaaaaatc cgaccattta ttttctgaaa
      541 ggctatcgaa aagtcgcttt agaactgaat gaaaacgggg aatttgagga atctaaaaaa
      601 taccaagcca catcggaaga ggaaaaagta gctgttcgtg aattacttga agaggctgcg
      661 ccaactgatt ctctgaagga gaaagaaaac gatcatttct atcgagtaga attcaacgaa
      721 aatgatccag aaaaaggatt gaaaagttat ggtggagaga tcgtaacatt agaattgatc
      781 gatcaactca aaacgttgga caaccaatac gctgcagaag caggttatta taaattctat
      841 tttgaagaag tgaagaacgg agaagtagtc gatcagatcc gaatggatct aggggatggc
      901 gtcaacagta atcaacccat ctatttagag ttagcaaaat tgtgcgaaca agtacctgag
      961 aaggaacaaa aacaatcggt caaaaatgaa cctgtttcga aagaacaaat gcagtcgaaa
     1021 gaaacaaaaa caaaacaaaa gggctatcaa aaacgaacgg cagaagagat taaacaagaa
     1081 gttaaatccc tttcgcaaca ggcactggat gcggtgaaaa agcataccca ttctcctgaa
     1141 gacgtcaaag aacttttgaa ctttatgtct cgttttccag aacgatcctt tagaaatcaa
     1201 ttattgattg aggaacaatt tccaggtgca acggcttgtt taggtcgtgc cgcgttgaaa
     1261 aaagaaggaa tctatatcaa aaaaggggaa caagggaaca agatctttgt cagaaaaacg
     1321 gtcaaagggt tttatgagaa aggacgtggc tttgttcgag aaacagaagc tacaccagaa
     1381 gataaaaaac gaatcgaaag tggtcaaatc gaagtgatca aaaaacctta ctatacgatt
     1441 gaaaaagtgt ttgatatcac acaaacacag ttaaaacctg aagactatcc gaaaatcttt
     1501 ccgaaccgtg tctttgattt tcaattggat aaaaaaggac agtcagaact tcaagctggg
     1561 attcaagcgg tagccaacaa tattggaata caaattcgag atatgacaga aagtgaagtc
     1621 tatcaaagag aattaggaca agcacgtggg gcgtatgttc gtaacgaatt tactggcgaa
     1681 gaagaaatcg tcatgaatac tcgaaatact cctacgcagc atttagctac aagtattcat
     1741 gaattagccc atgcaagaat gcacaaattt tcagaattag ataccgcaac taaagaattg
     1801 caggcagaaa tgacttctta tattgtgtgc aaacattttg gaatggacac gtcggagaaa
     1861 gctattcctt atattgcagc atggacgaag aatggtcaga cgttggatga taaggaagct
     1921 gcggaacgtg ggaaaatcat gaatgatgtc agccgtgtcg caaatgaatt cattcaaacc
     1981 atttctaatg aaatcaatca atacagagaa agagaaccag agatccaacc agcagaagtg
     2041 aagcaggagc ataaaaatac tttagaaacg ccagagaata agaatattgt gcccgttggt
     2101 tcactttgga tcgacaagaa agacgggaaa gtaatggaag aagatacggg tcgaacgtta
     2161 catttgaagg atgcggcgtt tattgaagat ccgcagcata atcaagtaat tttaaaagga
     2221 aaaagttatg gaaaagagat ccaaacggcg ttaccttcag aagaatttgc gctaggaaat
     2281 ccattccgat tttcacttga gacagtgtca gaagtggcaa aacgaaaaaa tgaaatctat
     2341 tatgaacaac aattgtcaca aaaagaacgt gtataaaaca agtaaaaaag actatctgaa
     2401 aaacggatag tcttttttct tttgggaggt tttaaaatga ccgagaaaaa aatgagtctt
     2461 attgatcgat gtaaacagat cgatatagtg gattttgctc gaaacaatgg aatggctgta
     2521 gtcaataaag gacgagatta tcgcttagaa gaccatgatt catttgtttt tgatcgaaga
     2581 aaacaacgtt tttattggaa cagtcaaaat attagtggcg acattattga attagctaaa
     2641 ctttttttca tagacaaaga gatccaagac cctaagcaac aatttaaagc agccactgat
     2701 tttattctaa agaatgaaga caaaacagaa cgagtggaaa atcttcattt tgaaacagaa
     2761 aagtacaaag atcaccccgt tgattatcaa ccactcacag aaaaaggaag gaattatctg
     2821 aaggaagaac gaaaactacc agagtggctg attgattatg cagaaaaaga agggttgatt
     2881 gccgaactaa agccaaaata tgaacggcaa aatttcttag ttcgagacga tcgtttagat
     2941 catgcggtcg cctttttatg gaaagatcca cagacaaaag agacagtagg ggcgagttat
     3001 caaggaacgt tcattgatta tgaacgtttt ggtgagagag gcacatataa gcatatagat
     3061 aaaaattcga cagcgaatca tggctttaac ctaaagatcg gtgaccctaa gcagataaaa
     3121 ttttttgaga gtagtattga tttacttagc tatgctgcac tgaatcgtga tcagttaaac
     3181 gatacgtggt tagtctctat ggaagggtta aaacaccatg tgatcagtca ttattttggt
     3241 gaggcagtat ctgaattgag aaagaaacaa gcctttcctc aatccatcga gatttgtgtg
     3301 gataatgacc gtgcaggaca tattttctac gaaaaagaac aattaatggg cgcagtcgat
     3361 ccatttacga atcagaaagt acgttgtgaa cgaggaatcg ctaatgattg gcaagtgcca
     3421 aaagaataca aaatcattta tgaagaagtc gcaaaagaag aaaaggtcac accagaagct
     3481 attatggcca tacacaagac agaaaacaat ctgcaattaa cgaaccaact ggtttccgct
     3541 cacaaagtaa atgcgttttt tggccagcag ctttctgtta acgattcaat agaagccatt
     3601 aatttgaaag atatttgtag agaagtcgca aaagaattaa aagtttgtga acgcgtggat
     3661 ggtacgtacg attttgatcg tttttatcaa gaaaaagggg atattaatgc gcagattctt
     3721 ttctcttata aagcagaaca atactacaaa ggctataaaa atcatgaaca tgaatttgta
     3781 cctgaagtaa aaaaggactg gaacgatcaa ttgaagcatg agatccagca acaagaaatc
     3841 agaaagcaaa aacgtgccat gttgtttcaa caaggcagac aacaagaaag ggagtgaaat
     3901 cgtcatgatg aaaaccgtta ttttagcaga aaagccgtca caagcgaaag cttatgccga
     3961 tagcttctct aaagccactc gaaaagacgg gtattttgaa atccaagatc gattgtttcc
     4021 tggtgaaact gtgatcacgt atggttttgg tcatttagtc gaattagatt cgcccgatat
     4081 gtacgatgaa aattggaaac aatggtcatt agaacatttg cctattttcc ctacacatta
     4141 tcactatcat gttcctaaag ataagaaaaa gcaattcaag gtggtgaaac agcaacttca
     4201 atcggccgac accattatta ttgcgacaga tagcgatcgt gaaggggaac tgatcgcttg
     4261 gacgatcatt caacaagtag gcgccgatca aggaaaaacc tttaaaagac tttggatcaa
     4321 ctccttagaa aaagaagcca tttatcaggg attccagcag ttacgagacg caaaagagac
     4381 ctatccaaaa tttgaagaag cacaagcacg tcagatcgcc gattggttaa ttgggatgaa
     4441 cggtagccca ctgtatagtt tactcttgca gcaaaaaggc ataccaggaa gcttttcctt
     4501 aggcagagtc caaaccccaa cgctttacat gatttatcaa ttgcaggaaa agattagaaa
     4561 tttcaagaaa gaaccttatt ttgaaggaaa atccgaagta atcgctcaaa atggcgcatt
     4621 tgatgcgaag cttgatccga atgaaaccca agcgacgcaa gaagcatttg aagattattt
     4681 aaaagaaaag ggggttcaat tgggaaaaca gccaggcatg attcgccaag ttgaaaccga
     4741 gaaaaaaagc gcggcaagtc cacgtctttt ttctctatcc agccttcagt ctaaaatgaa
     4801 tcagctcatg aaagctagtg ccaaagacac tttggaagcc atgcaaggct tgtatgaagg
     4861 gaaatatcta agctatccgc gaacggatac cccttacatt acggaagggg aatatgcgta
     4921 tctgttggat cacttagacg aatacaagca ttttttgagg gctgaagaaa tttcgactcc
     4981 tattcataca ccgaatagta ggtacgtgaa caacaagaaa gtccaggaac attacgcaat
     5041 cattcctaca aaaacagtta tgacagaagc gacgtatgaa agtctgacac ctttacaaca
     5101 agcgatttac gagcaagttt taaaaacgac agtggcgatg tttgcggaaa aatataccta
     5161 tgaagaaaca acgatcttga cgcaagtcca gcagcttcag ttaaaggcaa tcggaaaagt
     5221 accgatagat ctaggttgga agaagttatt tggcaaagag agcgacggaa aagagaagga
     5281 agaagagccg ctacttccta aagtgacaaa aggagaaatg gtgacggtag atctacaagt
     5341 ccttgagaaa gaaaccaagc caccacaacc ttataccgaa ggtacgttga tcactgcgat
     5401 gaaaacagct gggaaaaccg tggatagtga agaagcgcaa tccatattga aagaagtgga
     5461 aggaatcggt acagaagcga cacgggcgaa tattattgaa accttgaaac aaaaggaata
     5521 tatcaaggta gagaaaaata agcttgtggt gacgaataaa ggggttttgc tttgccaagc
     5581 tgtggaaaaa gagccgctgc ttacgagtgc cgaaatgacc gccaaatggg aaagctactt
     5641 gttaaagatt ggagaacgaa aaggaacaca aaccactttt ctagccaata tccaaaagtt
     5701 tgtctctcac ttattggaag tcgtcccagg acaaatccaa tccattgatt ttggatcgac
     5761 gttacaagaa gtgaaagcag ccagtgaaaa gcaagaagcc gcgcgtcatt taggaatttg
     5821 tccaaaatgc caggaacaag aagtcttact ctatcaaaag gtggcagctt gtacgtctga
     5881 agcctgtgat tttaaattat ggacaacgat tgccaaaaag aaactcacag cgacacagct
     5941 gaaggagatt attcaaaata gccgaacaag ccaaccagtg aaagggttaa aaggtcagaa
     6001 gggttcgttt gaagcaacga ttgttttaaa agaagacttc actacaggct ttgaattttc
     6061 tgaaaagaaa aaaacgaact tcaagaaaag aacacgacgc acgacaaaat aatggagggt
     6121 taatcttatg cgtaatacaa cacacaaaca acaagaaatc gaaaatcaaa aagccaaaat
     6181 tcgttcagaa caagagaaat tacgtgccag taaaagaaga cttcagcaat taaagaacca
     6241 gcaacggatt caaaaagaaa tcgaattaga tcgatagaca ttaaccttgt tacaaataca
     6301 ctatcatgat atatttaatg tagtgtattt gtaacaaaag gggggaagta aatgcaagag
     6361 aaactgaaag aattgttggc tattcgcgat ggaaatttat ctatgaagga agccagaaaa
     6421 ttaggaatcg cagcaaccac tgtacaaaga ttggtgaatc aaggaaaatt gatcaaagtt
     6481 gatcgtggtt attatgtaga ggatggtcat ggaatagatg atttattttg gcttcagcaa
     6541 cgtttttcca ggggaatctt ctctcatgaa acgactttag atatttacca gctttctacg
     6601 aatatgccga aattcattca cttaactttt ccccatggat ataacgttga tcgttcaatt
     6661 acaaaagagc agcagttaca atttcatttc gtgaagaaag aagtttatga actaggaaaa
     6721 gtagagggaa cttcctttca aggaaatcct attactatgt atgataaaga gcgtacactt
     6781 tgtgatattt ggaatccgag atatgaaatt gaatatgaaa tgaaattaga agcattaaaa
     6841 gagtacatgg aagacccatt gagagatcca agtaaattaa gagattacat gacaaaacta
     6901 catgtagaaa aagaaatgaa ataccacatg caaagcttat attgatggag gaaaaagatg
     6961 acaccgacac aatttgcagc aaaaacaaga aatttagcaa agaggacagg attaaatgca
     7021 cagttagtac aacggcattt tatgatggat cgtttaattg aacaaatttc tgaatctaag
     7081 tataaaaatg atttcatttt gaaaggtggc tttttattag gatcaaaata tggaatcgat
     7141 agaaggacga caattgatat cgacacgaca tttagaaata gcaaattaac tgaagaaaaa
     7201 ttgaagagta tttttaatga tttaactagg aaacccacga aagaaggtat tgttttctct
     7261 attcaaggaa tgaaggaaac aagagaagcc gatttttatc ctggatttca aattaaattg
     7321 gtggctaatt tagagaaagt acgtgtgcca tttaaaatgg acgtgacaac aggagattct
     7381 atttatcctg aagtagatac acactatcac caattaatgt ttgaagataa aaaggtggag
     7441 attccagctt atccaacagc acaaattatt gcagaaaagc taagtgctac gttcagcttt
     7501 ggcacagata attcgagagc aaaagattac tacgacttat ttaccattcc gaaactagaa
     7561 aatatcgata cgaaagagtt atacaaaagt gttcgaaata catttacaaa acgtggggat
     7621 acaaaaccgt tgtcctttta ttatgaaaga gaaatggata agatcatagg aaatgactat
     7681 ttggaaaagc agtgggaaaa atatcgctca gttaacacgt ttgcgaattc catttcattt
     7741 acagatacag tcaacgaaat tgatcaccta atgaaaagtg tgatcaaaat tgaaaatcaa
     7801 gaaagaaaac ggcagtttaa aagatcacaa caaatgaatc aagaaatgga gagataaaga
     7861 tgatcaataa tgtaacttta gtaggacgct taaccaaaga tccagattta cgttataccg
     7921 cgagtggtac tgcagtagcg acttttaccc tagctgtcaa ccgaaatttc acgaatcaaa
     7981 acggcaatcg agaagctgat tttattaact gtgtgatttg gcgcaaacca gcagaaacaa
     8041 tggtgacatt agctaaaaaa ggtagcttga ttggtgtagt tgggcggatt caaacgcgga
     8101 cttatgacaa ccagcaagga caacgtgtgt atgtgacaga agttgtggcc gataatttcc
     8161 aactattaga aagcaaagca gcgacagaaa gccgtgcgca cgctgatcaa tcgtccacca
     8221 gtccaagcac caccactttt gaacaacggg atacagcgac gcctaataac aatggattga
     8281 acgctttaca gaatccattt gggaatccat ttggtggaca atcaatcgat attagcgatg
     8341 atgatctacc gttctaagta gcattttcta ttggttgcat ttgctacggt ttatgttata
     8401 atttagtcaa caaaaagaat tttcaatcta taaccaaaaa ccactagctg gcgggctagt
     8461 ggttttcttt tacgtttcta ttataagaat taacgggtag gcttactgtc tttcttggca
     8521 aatttacaat caagccattt gcagatatag tgcgcggcta cttctgccat gattgcaaaa
     8581 acaaaatcaa aaagaatttt caaacctata acctcctccc cttgccagat taggccgggg
     8641 tagcagacag caagactatt atagcataat tgaaatatcc aaatgattgg atttttgcta
     8701 taattttagc gaggtgattg tgatgaacaa atatgttggt ttgcttgata aaatccgagt
     8761 aataaaaacg caaccgttat tggttcgctt tactttacaa acgattcatg aatcgattaa
     8821 ttgtgtagtt gcagatattg aaattatcga taaattgcta attatggatg atggaaaata
     8881 caatattgcc gtcacaggtc acttcaataa acgaaatcag ctagttattg ctagcatgta
     8941 tgttagaaat cccgatcatt ttacgcgatc gatgggcata taaaaaaaga agagttgcaa
     9001 tcctaatgta ggactgcagc tctttttgtt ttacatagaa acaagaaaaa tgaaaaggcc
     9061 tttttaggaa ggcgcgagcg ccttctcaat tgaaaaacct ctctcactcc aaccgccacc
     9121 gccgttcgag aagaaaggaa gtgaacgttg cacgttcact aagataaagg gtcttgcaaa
     9181 gcaagacaaa atagaaaaaa cttcctctca ccaaaaggtt cgttcctgtt cgttatctct
     9241 ttattgattt gccagaccgg caacaagttg ccttttatca ccaaaaagcg acaaggacaa
     9301 gggtaaatgg cacgctgcgc gcccttgtcc ttgtcgcttt tcgttgattc attctggcca
     9361 acaaatcaat aaagaaagaa ctaacgaaag gaagtaaaac ctttcctaag aggaggcaga
     9421 atcaatcatg cgaaagaaat ttgattattg gggtgttccc ttcagcgaat tattcccaaa
     9481 ctatcatgca ccacatactg tcgaatgcga ttgtggacaa agagcgaaat gtatcaaaag
     9541 ctatcgtttg tatcaatgtc caacatgcgg aaaaaaatac accttgtctt atggcgatta
     9601 tattttgatt gatgagaaag gaaagaaacg ctgatgaaaa cataacataa attattgact
     9661 gtaaaagaag tcgttcaaat tttggtggac aaagaagtca tttctgaaaa agtagcagaa
     9721 gctttactta ttccgcggta ctggacgata gaaacccctt tagtagagga tgcaccgact
     9781 atgctatttg aagacctagt gaagattgca ggatatgaac tgcattatca agaaaacatg
     9841 gaaacttatg aaaacgaata ttggttaacc aaattagaga actaaaaagg agaaaaaaat
     9901 atggctcaaa attactatga cgaatttgtc aaattacctt tggataaaat ggcacaaaaa
     9961 atggaggata tgacattcct ataccatgag acacgtgttc caaaaaaaca ttataaggaa
    10021 agattgtcgg tagctgtgga agaaatgatt gaatcaggtg tcgaaatgaa tctcattgcg
    10081 acgtactatc gaacactgga agaactgaaa aaacaaaatg caaaatggtt tttccaagcc
    10141 ttactatgct tggaagtagg tgtcaaacca agcactatta aaccttctga atatcaagct
    10201 ttagaactaa cgtatgggaa atttgtcgaa acgaaaaaag ccaagactgt ttcaagtgaa
    10261 tggttagatt attttgaaaa tatcaataag tatggcgcct attacaccat gaaaaaggag
    10321 gacaacgaaa atgaataagg tgatttatca actaacaacg gaagatgtgt cacacttttt
    10381 aacaaacaaa gaacatttga atgaagtaat cagacggatt caaaaagact ttagttgtat
    10441 ggaagaaatg aagtacttta ttcaaagagt cgaagaacaa ttgtatttgg aaagtgaaac
    10501 gagtcaaacg aaggttacag tcagcaataa ttatttagac gtgtataaac atcgtcctga
    10561 aacggtaaaa aaaggaacgg agcttctacg actagaaaca gaaaaataca tcatgaatgt
    10621 ttacctagaa aggagtgaac tggtcgaaaa ctttgtcagc tgcacgattt gcaggaaaca
    10681 ctattactca gaatcagaat attattcaat ttttgagagc atggaattaa tggaagaaga
    10741 aatggaagag ctaaacagca aaaatgcgac gatagacgat tattatcttg tatttactag
    10801 atttttgacg tgtggaacgg aatctatcgt gttaatggga atggattatg aactcgctgt
    10861 acccgaagca tttttgaggg aatatgtaga agaagtagat aacccaggcg acgactggta
    10921 cacgacttta actgattttt tagataccta cacttacgac gatacagaag aaatccaaaa
    10981 tgaacttgac cgtaaacgaa tgccatatca acgatttgaa aggaaaaaaa ggcaataatc
    11041 atttataccg aaaagtgaga atgtgtgaaa taagaacaaa aaccttgaaa ttaagggaat
    11101 ggttcttatt tcgcaccaat cgctccaacc gcccccgccg cttaggtggt gcgaaataag
    11161 cgggcaaagc ccgataacct aagagaaaga aggagatcca tatgaataaa atcattcctc
    11221 tgttgttcgt actgtgtatt ggcgagatac ttatgcgtca tggtgcagct gtacatttaa
    11281 acttcgtagt attcttagga atactagtat tttgtttgat tttttggcgc tttgtgttag
    11341 cccttgtgag tctactattc aagttgattg cagccatatt tttcataggg ttatttcgat
    11401 gaaaggagag agtactaatg ccaattgcaa taggaaataa acggttaccc gtgacattag
    11461 atgaaaagag acaaaaagaa ttgcagcaac taaagcagaa gtacggaaaa agtgaatcta
    11521 agattatgtg tattgcgtta gatctattga tcgcgcaaga aaaagcagga tttgatatac
    11581 cagcactaaa aaagtgacgt cacttttcat ttgaagaaac aaaagtgata gcacttaatt
    11641 gttcctacta tttagaatat tttctatgtc ctttttattc cattttgtat caataactat
    11701 gaaatcttta aagtatccag atcaattttt ttcttacctg attctaaaga ttttgttttc
    11761 ttaaacatat tatgatttcc tttcgactat tttcatataa atgttaacat agtttatgga
    11821 ttcatggaat gttaatctaa tttaatagaa aaggataatt aagtatatac gttttttctt
    11881 atgtatgtta tactatgtat atacagaaag aggtgtctac tatggaaatg ttacctataa
    11941 aaaaatgggg aaatagtaat ggccttagat tgccaaagta tattatggaa tatcttggaa
    12001 ttcatacaga agacaaagtg aagataatac aagaagaagt aaacggtcag aagcggctaa
    12061 ttatcgaagc ggctaatata gaaaatgaat taacaattga acagttattt gaaaattaca
    12121 ctgatgaaag aaatcatgtg acgattcaaa atttaggtga agcagtaggt aatgaaaaat
    12181 ggtgagaaaa cctagacaag gcgatattct attattgaat acagcacctc gatctggaca
    12241 cgaacagaca ggtaaaagac cttatattgt tttaagtcat gatattattg cggattatag
    12301 taacgttgtt acagttgctc caatttctag tacaactaga aactatccgc tatatgtaaa
    12361 tataaactca gaatatgaaa tgaagacaac aggaaaagta ttattagatc aactaacgac
    12421 aattgattat gaagccagag agtgtatttt tttggaaaaa gcgcatgata gtttagttga
    12481 ggagttattg atgaaagtta gaacagtttt tcaaaaagta agcaaataaa atagaaacag
    12541 cagcatgatc aactattcgt gattgaccat gctgtctttt tttattctat atacggtttg
    12601 tctcgtaaca ttgtagtctt t
//
LOCUS       DASHHB010000073        12431 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.076, whole genome shotgun sequence.
ACCESSION   DASHHB010000073 DASHHB010000000
VERSION     DASHHB010000073.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12431)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12431)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12431
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.076"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..190)
                     /locus_tag="U2K71_002421"
     CDS             complement(<1..190)
                     /locus_tag="U2K71_002421"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316447.1"
                     /translation="MKEQRFDKNVYMEFFYWLYQSLASSLCFWCVNILYIAAAFLLSI
                     DGRNVFSFIFSLLFIGPGC"
     gene            complement(200..1015)
                     /locus_tag="U2K71_002422"
     CDS             complement(200..1015)
                     /locus_tag="U2K71_002422"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM7316448.1"
                     /translation="MPFIQWLGMILLIVLALLTIIPFLWMVSASFKANNEVFTIPIQW
                     IPKTWHLENYLVIWSRIPLVTFFKNTAFLSVVITLIQLFTSSFAAYGFSKLNFKGRDI
                     LFLAYIGTIAVPWQSYMIPQFIMMRKVGLTYTLWSLVLLQAFNAFGVFLLKQYYSSIP
                     DSLCESARIDGLSEWGIYRKIILPLTKPAIASLTIITFVNTWNDYMGPFIYLSSTENK
                     TIQLGLKMFIGLFDAEYALIMAASVVSVLPVAFIFLAMQKYFVEGIASSGMKE"
     gene            complement(1277..1720)
                     /locus_tag="U2K71_002423"
     CDS             complement(1277..1720)
                     /locus_tag="U2K71_002423"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator
                     substrate-binding protein"
                     /protein_id="HEM7316449.1"
                     /translation="MLDPVDTEKYETILLPRKEKWGLLILENSVLAKKDVITKEDFAN
                     VPLLISKRPEIQRMFADWLGIKKEQLLITGTYNLIFNVFALIENQVGAALVIEGAVSH
                     LSKRVTFVPLDPMIETNCMLVWKKNRILSPVVNELIHRFKEAFEQ"
     gene            complement(1854..2192)
                     /locus_tag="U2K71_002424"
     CDS             complement(1854..2192)
                     /locus_tag="U2K71_002424"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM7316450.1"
                     /translation="MYQVKKSRSIAMELRLLKYFWTIAEEGTISQAAEVLHLTQPTLS
                     RQLKELEEELGTELFVRENRKMILTEAGYFLKKPSRRNFRFNESNHSRIREQKKTIIQ
                     RPIYDRLCGS"
     gene            complement(2180..2557)
                     /locus_tag="U2K71_002425"
     CDS             complement(2180..2557)
                     /locus_tag="U2K71_002425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HEM7316451.1"
                     /translation="MNIKQVSEEKGISADTLRYYERIGLIPPVNRTKGGIRDYTEEDL
                     KWVDFTICMRNAGLSIESLIEYIRLSSEGEATVFERRKLLIEESELLTKEIAEMQECL
                     ERLQGKIKKYDRVLSRNKMECIK"
     gene            2781..3974
                     /locus_tag="U2K71_002426"
     CDS             2781..3974
                     /locus_tag="U2K71_002426"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7316452.1"
                     /translation="MTTYQRKVLLSTSAGIALENMDIMFLAFSLSSMITSFHISGTQA
                     GFISTITNLGMLIGGIFFGIIADKYGRVKVFSQTVLLFSIASLLMYFASNIYLVYLFR
                     FIAGVGAGGEYGACMSLISESFSKKQIGRASSVAGIGAQVGAALAAILAAVIIPTFGW
                     KMLYVVGVLPVLMVLFIRRGLTEPKEFKSVQSAEKKTKLSRLFESKHLAWQTCGLSIM
                     VTVQIAGYFGLMNWLPSIMQNRLGLSVSGSSLWMVSTILGMSLGMMTFGIIMDKLGPK
                     LAFSLFLICSSLSVYLLVLAHSQWSLVAAAVIVGYFINGMYGGYGAVISSLYPTEIRA
                     TANNFIMNLGRAVGGFSSIVIGFLMDHYSLTAVILFLSGIYLVSLLVLTTLTGVTALR
                     ESLQH"
     gene            complement(4140..4562)
                     /locus_tag="U2K71_002427"
     CDS             complement(4140..4562)
                     /locus_tag="U2K71_002427"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7316453.1"
                     /translation="MQTFLTKVSQKKIKMIHLLLETERWCSIEELQNELKVSSKSILN
                     YLTDLEELFQKYPDKVILKNENNRRFSMEKQENFPIYIIYLHFYRKSYNYHLIEFMYQ
                     HPEKNLEDYADAQYTSVSTVFRYAKLLVKYFEMTDVKE"
     gene            4956..5150
                     /locus_tag="U2K71_002428"
     CDS             4956..5150
                     /locus_tag="U2K71_002428"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316454.1"
                     /translation="MVAGSLLSGCGATTSTTQSTSSDQASSSSTTISESTADSSKEQE
                     QIEKKKEEKQKAEKAALEKK"
     gene            5147..5593
                     /locus_tag="U2K71_002429"
     CDS             5147..5593
                     /locus_tag="U2K71_002429"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316455.1"
                     /translation="MTEAESALSQAEANPTKENYATASAAIQALPENKQELTSRLAAI
                     DSTIKAKEAEEERKKQEEIARQQAEQAQQEAIAQQQAEQARQEAIAQQQAEQARQQSA
                     AEQASQQAQAAAPEQNNEQTVYVTPTGSKYHTHKCGNGTYTPATLS"
     gene            complement(5803..6975)
                     /locus_tag="U2K71_002430"
     CDS             complement(5803..6975)
                     /locus_tag="U2K71_002430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class C sortase"
                     /protein_id="HEM7316456.1"
                     /translation="MNKSRIRILDIFMAIILVVGIGIFSYPFVEDSLNDFLAQQMIIH
                     YQKQASNKNSEEIKKQQEKMTKKNQQLAEQNVSPGIGSFNQAVDAKALKDLPSNAFFM
                     EHMLGVIEIPKINVSLPIFDQTTEIFLQKGTSLLEESSYPTGGKNTHAVLSGHRGLPE
                     AKLFTDLPKLKKGDQFFIQINGKTLAYQVEKIQVVLPDEVDSLGIQKGRDLVTLLTCT
                     PYMVNTHRLLVTGHRIPYQAKEAKKAIQGIDQWKKWKFFIWFIGILLGSIGLVWLLIA
                     YLDSLAIAKRNYPLSFYVKNTNGRPIEGMVFSVKTLNGKHYITREKVPFVKASDEYGL
                     VRFSDLKGRNYRLQHEELLLKIHVKHKHSKQFSMKLKKGRYKLRKEKEVYYLIEKE"
     gene            complement(7181..7996)
                     /locus_tag="U2K71_002431"
     CDS             complement(7181..7996)
                     /locus_tag="U2K71_002431"
                     /inference="COORDINATES: protein motif:HMM:NF033902.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SpaH/EbpB family LPXTG-anchored major pilin"
                     /protein_id="HEM7316457.1"
                     /translation="MTGFDENTGVALYDPAESALTFTTDTEGSISVNGLIDGNYTLVE
                     TKAPDGYQLTNPQKETTFIVKNGKLQQTTGIIHGADEANLGNPNGVVNRENTTTDNTI
                     TVENVQNYGDFHFIKQDVNTQEKLADAEFSILRAKDPTVKLMKKIANLASGEYPYAWS
                     DEVTGSDWEEVKLISDSEGKFSIEGLKQGAYCLQETKAPEGYALPKNEAAYTEFTVDD
                     DPTTSDETTIDNQPKGILPSTGGMGIIVFILVGTALVGGAVIYFKKRHQETEA"
     gene            complement(8146..8592)
                     /locus_tag="U2K71_002432"
     CDS             complement(8146..8592)
                     /locus_tag="U2K71_002432"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEM7316458.1"
                     /translation="MKNIKTKIWTFLGTAIMLLPFVLGLGTAEVSAAVSPTPENVTVN
                     LHKLKFTSAPENQINNGTELTFPNSEPLNGVEFNVYDITATYYPSKDTAVPADATPFA
                     SVTTSGEGLANLTLPGKSDGKDAVYVFVETPKPGVETSPNIVLSLP"
     gene            complement(8589..9593)
                     /locus_tag="U2K71_002433"
     CDS             complement(8589..9593)
                     /locus_tag="U2K71_002433"
                     /inference="COORDINATES: protein
                     motif:HMM:NF037639.1,HMM:TIGR04226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isopeptide-forming domain-containing fimbrial
                     protein"
                     /protein_id="HEM7316459.1"
                     /translation="MTDSSNHKLTTIHLYPKSEQKIPAALTLTKTVENKQTDFADGDK
                     VPYLITVTIPENINEIGTYTIKDTADPQLWLEPETIDFLIGGDKIHTFHTQKIKHGFV
                     LSDSGKDLSSFAGKKLTIRYQMTLKENSEKTTFDNDVRLTTDNQTVETHLAIQTGGKQ
                     FVKVDRQTKQKLADAQFLVKKNDQYLAQENGINHWVAKDDSRATIFTSSKDGTFSVHG
                     LSFGSYELVEIKPPKGYILSQSTILFEVEKGSYDPLHPIPLEIINEPKANPTNNGKTP
                     VQMQTNGPSNEKNGGSFPKTSEEMLGGFSILGLLLVLSTGTAWFYKKQQKGNNRREIK
                     "
     gene            complement(9633..9962)
                     /locus_tag="U2K71_002434"
     CDS             complement(9633..9962)
                     /locus_tag="U2K71_002434"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HEM7316460.1"
                     /translation="MFESDKMPSISQNDGINIPDNFQGVKGLNGVTFAIYDVSDEFYK
                     LRSEGSSVEDAQRKLAQKSDSEKILAENVTKTVDEEEGIASFSASDKDEQGRDAVYRF
                     TEIKTSD"
     gene            complement(10088..12415)
                     /locus_tag="U2K71_002435"
     CDS             complement(10088..12415)
                     /locus_tag="U2K71_002435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM7316461.1"
                     /translation="MKMIKKTQNKIQRICQKHFVSRLKAVSKRKYLHPIMISIILIFT
                     FLGSLSSIYVNVYTETSNFANQTTEETNSPPYSEIQSIESTNQSSNNETTSTTSSTQL
                     IDPTEKEITAAKAAGEKAYSETGRSQTFSTVAATANFNGHTIPDGYTVIYAKDGVIVA
                     VNGSMSADKTALQGVTKAIAFIDNRPDIHNLFIGSTVNGTTITFPKSIYMGVATAGNT
                     IDSVTPNADKSYSFTPSTWGGLPAGGGMSTFSIGIQLANDIPFTGYSIKNGQIVDGKG
                     NVSNFPTYQAATVQVNANRTGTFSYTVNGGAAKIGTYGTTLSGIHVGDKVVITPTAVT
                     GYSFAQTNGSFTALESNVDTVTYTGKQTPITIKYQDYFGQMIAPSKIMNLTYGSAAQD
                     LTTNTPTINGYTFTAVSAIETKNQSAMSVLASLDTNGNVIVKDANGKQISEVILYYKT
                     NATVSINANGSKYYDGLSVVPIVKYAFNDKTESTSLLNNDLSIDQITWNPTDFKAMNE
                     KGVEVTAPTEIGTYTSWQLTKSGLAKLAARTNYLFTIVQTSDVYTIKQISGAVELSGS
                     KTYDGKAGVPSIHVKLAEGVTSSLTPVQVALSSMDYTADAQSAKNMVNAGSYTIKLTN
                     SGIDKVKAANPKLSFTDIANTSGTYTIYKANAVITVDDAAFNYDAQSHSISVGNLHVT
                     GVVLGESLDYTLTVNSRTDIGSQIVTISLGEGSVNNNYTIIATDTAKLTINPTLLLPS
                     TGGIGLLPFVFLGLIFIFSGFFYFIHRKKKAGEQR"
BASE COUNT         3513 a         2430 c         2272 g         4216 t
ORIGIN      
        1 tgcaacctgg accaataaaa agtaaagaaa aaataaagga aaagacattc cttccatcta
       61 tcgacaaaag gaacgcagct gctatataca agatattgac acaccaaaaa caaaggctac
      121 ttgccaatga ttgatacaac caataaaaga attccatata aacatttttg tcaaaacgct
      181 gctctttcat aatcacctcc tattccttca ttcctgatga agcgatccct tctacaaaat
      241 atttttgcat cgctaaaaat ataaaagcaa caggaagaac agaaacaact gaggcagcca
      301 tgattaatgc gtactctgca tcaaatagcc caataaacat tttcaaccct aactggattg
      361 ttttattttc cgtagaagac aaataaatga atggccccat ataatcgttc catgtattaa
      421 cgaatgtgat gatcgtcaaa ctggcaatcg ccggttttgt aagaggtaaa atgatcttgc
      481 gatagattcc ccactctgat aagccatcga tccgcgcact ctcacataaa gaatctggga
      541 tactactgta gtattgcttc aacaagaaca caccaaaagc attgaacgct tgtaatagca
      601 ccaacgacca caacgtatat gttaaaccca cttttctcat cataatgaac tgcgggatca
      661 tataagactg ccaagggaca gcaatcgtgc cgatatatgc taaaaataaa atatcgcgcc
      721 ctttaaaatt tagtttagaa aacccgtaag cagcaaaact agaagtaaac aactgaatca
      781 acgtgatcac aacgcttaaa aaagctgtat tcttaaaaaa tgtcactaaa ggaatacgag
      841 accaaataac cagataattc tctaaatgcc aagttttagg gatccattga atcggaatcg
      901 tgaacacttc attatttgct ttgaatgaag cagaaaccat ccataaaaaa ggaatgatcg
      961 tcaataaagc taatacaatc agcagaatca tacccaacca ttgaataaag ggcatgtttt
     1021 ttgaacgaat ttttgctttt tttctttcat ttcgaacacc tccaaagtta taatcttcat
     1081 ttctttgtaa tgattacaat ccagaacaac ggttattata gctgattcat ttccgttctt
     1141 cttatgtttg taagcgctta tttaacaagt tatgtaaatc tctttatttt ttttgattta
     1201 aaaatgaaaa aatacaaaag caagactgtc atacgtctcg cttttgtatt gaaatttctt
     1261 cgattagaga tacttattac tgttcaaaag cttctttgaa acggtgaatc aattcattga
     1321 ctacaggtga taaaatccga ttctttttcc agactagcat gcagttcgtt tcaatcattg
     1381 ggtcaagcgg aacgaatgtc actcgctttg aaagatgact tacagcacct tctatgacta
     1441 aagcagcacc tacctgattc tcaattagcg caaatacatt gaaaatcaaa ttgtacgttc
     1501 ctgtgatcag caactgctcc ttttttatgc ctaaccagtc agcaaacatt cgctgtattt
     1561 ctggacgctt tgagatcaaa agaggtacat tcgcaaagtc ttcttttgtt atcacgtctt
     1621 tttttgctaa aacagaattt tctaaaatga gcaatcccca tttttccttt cttggtaaca
     1681 aaatcgtttc gtatttctct gtatctactg gatctagcaa aatcgctaaa tctaacaggc
     1741 ttttttctag tcactccgtg atatcatcac ttgttccgct gtggatatga aattgaacgc
     1801 tgggataatc acggatcaac ttttctagta atagtgccaa tgtattcgaa ttatcagctt
     1861 ccacacagcc gatcgtaaat cggccgctga ataattgttt ttttctgttc tcgaattctt
     1921 gagtggtttg attcgttaaa tctaaaattt cttctgctcg gcttttttag aaaataacct
     1981 gcttctgtca agatcatttt gcggttttcg cgtacaaaca gttctgttcc tagttcctct
     2041 tctaactcct tcagttgtcg gcttaaggtt ggttgtgtaa gatgtagaac ttctgcggct
     2101 tgagagattg ttccttcttc tgcaattgtc caaaaatatt ttagtaagcg taactccata
     2161 gcgatgctcc ttgatttttt tacttgatac attccatttt atttctagat aacacacgat
     2221 catatttctt gatttttcct tgtaaacgtt ccaaacactc ttgcatttca gcaatttctt
     2281 tcgtcaaaag ttcactttct tcaatcaaaa gcttacgccg ctcaaataca gttgcttctc
     2341 cttcgctaga taggcgaata tattcaatca aagactcgat cgataatccg gcatttcgca
     2401 tacagatcgt aaagtcgacc cattttaggt cttcttctgt ataatcacgg attccgcctt
     2461 ttgtccgatt aactggtgga atcaacccga ttcgttcata atagcgcaat gtatcggccg
     2521 aaatgccctt ttcttcactg acttgtttga tattcatctg atcactcttt ctcttaattt
     2581 cttgccactt tacttatgta ttttatttat tctacaactt taagtgtact ccaagtcaat
     2641 actcatttct attaaaacta agctggatta tctatatcgg ttgtttgtgt attatttcaa
     2701 ataaaaaagt cttgtttttt tattcttttt tggatatact tttaacaatt tagggaaatt
     2761 ttaggaaaag gggtataaaa atgacaacgt atcagcgaaa ggtattatta tcgacatcgg
     2821 cgggaattgc tttagagaat atggatatta tgtttttagc tttttcatta tcttcaatga
     2881 ttacttcttt tcatattagt ggtacgcaag cgggtttcat ttcaacgatc actaatttgg
     2941 ggatgctcat tggaggtatt ttttttggca tcatagctga taaatatgga cgtgtgaaag
     3001 tattttctca aacggtcctt ctgttttcga ttgcttcttt attgatgtat tttgcttcaa
     3061 acatctactt ggtttatctc tttcgcttta tcgcaggtgt aggagcggga ggagaatacg
     3121 gggcgtgtat gtccttgatt tccgaaagtt tttcaaaaaa acaaattgga cgagcttctt
     3181 cggttgctgg tattggtgcg caggtcggtg cggctttggc tgcgatttta gctgctgtga
     3241 tcattccaac gtttggttgg aagatgttgt atgtcgtagg tgtactgccg gtgttgatgg
     3301 tcttgttcat tcgtcgcggc ttgactgaac caaaggaatt caaatctgta caatctgccg
     3361 aaaagaaaac caaacttagc agactgtttg aatcaaaaca tcttgcttgg caaacatgtg
     3421 gccttagtat catggtaact gtccaaattg ccggctattt tgggttaatg aattggctgc
     3481 cttcaattat gcaaaatcga ttagggcttt cggtttctgg gtcttcattg tggatggtca
     3541 gtactatttt aggcatgtct ttggggatga tgacttttgg cataatcatg gataaattgg
     3601 ggcctaaact agccttttct ttgtttttaa tttgttcatc cttatctgtc tatctacttg
     3661 tgttagccca tagccagtgg agtttggtag cagccgctgt gatagtcggc tactttatca
     3721 atggcatgta tggtggatat ggcgcagtga ttagtagttt gtatccaaca gaaatccgcg
     3781 cgacggcgaa taatttcatt atgaaccttg gacgagctgt tggtggtttt tcttctatcg
     3841 tcatcggttt cttgatggat cattatagtt taactgccgt aatcttgttt ttgagcggta
     3901 tttacctagt cagtctgctg gtcttgacaa ctcttactgg tgttacagca ttaagggaaa
     3961 gcttgcagca ttgatagttg agtctgtaaa tgacaagaaa gaggtgtggc aaaagcttgc
     4021 atctgcggta ataagaccag ccaaatcaaa aacatgagac actgtttccg tttggctggt
     4081 cgtattactt gatgcagggc agctttttca actgctaatt tccttctatc tggcggcgac
     4141 tattctttga cgtccgtcat ttcaaagtac tttactaata acttagcata acgaaaaact
     4201 gtggatacac tcgtatattg agcgtctgca taatcttcca aatttttctc aggatgttga
     4261 tacatgaatt caatcaaatg ataattatat gattttctgt agaaatgaag ataaataatg
     4321 tagattggga agttctcctg cttttccata gaaaagcgcc gattattctc attcttcaaa
     4381 atgaccttat caggatattt ttggaacaat tcttctaaat ctgttagata attcaagata
     4441 gacttacttg ataccttcaa ttcgttttgt agttcttcta ttgaacacca acgttctgtt
     4501 tcaagcagta aatgaatcat tttaattttc ttttgagaaa cttttgttaa aaaagtctgc
     4561 atattttctc ctcctgtatc tactttacta tacacataca agaaaaaaaa cggatacaaa
     4621 tatcatattg tcacaccgtc aatagtgcat aaacctgtta ttatttctct ttttttccta
     4681 agtattctat tgatatgaat catttaatcg tttattatat aaatgtcaat aatctaataa
     4741 taaaataacg tacatatttt attattgcat acttaataac agaactatag caaaaaatcc
     4801 cgtgtatcaa cacgggatta gattaagttt cctcaatcac tattcataac acaattatta
     4861 taacaagtaa gtaaacaatt gaaaaggaca tttattgaaa acggttgttt tttacttaag
     4921 gaggaaattt agcgaaaaaa ttagttgcat tgtttttggt cgcaggaagt ttgttgagtg
     4981 ggtgtggtgc tactacgtct actactcaaa gcacaagttc agatcaggct tccagcagca
     5041 gtaccacgat ctccgaaagt acggcagatt catcgaaaga acaagaacaa attgaaaaga
     5101 aaaaagaaga gaaacaaaaa gcggaaaaag ctgcactaga aaaaaagtga cagaagcgga
     5161 atctgctttg tcacaagctg aagcaaatcc tacaaaagaa aactatgcaa ctgcttcagc
     5221 agctatccaa gcgctacctg aaaacaaaca agagttgaca agccgtcttg ctgcaattga
     5281 ttccacaatc aaagcaaaag aagcagaaga agaacggaaa aagcaagaag aaattgcacg
     5341 acaacaagca gaacaagcgc aacaagaagc aatcgcacag caacaagcag aacaggcgag
     5401 gcaagaagca atcgcacagc aacaagcaga acaggcgcga cagcaatcag cagctgaaca
     5461 ggcctctcag caggcacaag ccgcagctcc tgagcaaaat aatgaacaaa ctgtctatgt
     5521 cacaccgacc ggttcaaaat accatacaca taaatgtgga aatggtacgt atacccctgc
     5581 cactttaagc taagctcaag ctcgtggttt aaccccttgc tccaaatgtt atggaaacta
     5641 attcaaaagt tatactataa taataaaatt cttctgaaac ataaaaaaat agaagatggc
     5701 gttccagata gtaaaccaaa aataacagtt tacacgtcca tttcgtcagc ttctctcttt
     5761 tttcatttag tctgttccct ctgctttttt ggacatcttc attcattctt tctcaatcaa
     5821 atagtacacc tctttttctt ttcgcagttt atatcttcct tttttcaatt tcattgaaaa
     5881 ctgtttcgaa tgtttatgtt ttacatggat tttaagcagg agctcctcat gttgcaagcg
     5941 atagttcctg ccttttaaat cagaaaatct cactagtccg tattcatcag aagctttcac
     6001 aaatggaact ttttctctag taatatagtg tttgccgttg agcgttttta cagaaaagac
     6061 cattccttcg attggtcgtc cgtttgtgtt ctttacataa aaagataaag gatagtttcg
     6121 cttagcgatg gccaaagaat ctaaataagc gatgagtagc caaactaagc cgattgatcc
     6181 aagtagtata ccgataaacc aaataaaaaa tttccatttt ttccattgat caattccttg
     6241 gatcgctttt tttgcctctt ttgcttgata aggaatacga tgccccgtca caagcagtcg
     6301 atgtgtattg accatataag gagtacaggt aagcaaagtc acgagatctc tgcctttttg
     6361 gatacccaaa gaatctactt catcaggtaa gaccacttgg attttttcta cttgatacgc
     6421 caatgtcttt ccattgatct ggatgaaaaa ctggtctcct tttttcaact taggcaaatc
     6481 tgtaaataat tttgcttctg ggagtccacg atgcccagaa agaacggcat gggtgttttt
     6541 cccgccagtc ggataagaac tttcttccaa taatgacgtt cctttttgca aaaatatttc
     6601 cgttgtctga tcgaatatag gtaagctgac attgattttt gggatttcga tgactccaag
     6661 catatgttcc ataaaaaagg cattactagg taaatctttt aatgcttttg catcaactgc
     6721 ttggttgaaa ctgcctatcc ctggtgatac gttttgctct gccaactgtt ggtttttctt
     6781 tgtcattttt tcctgctgtt tttttatttc ctctgaattc ttattcgatg cttgcttttg
     6841 ataatggatg atcatttgct gggcaagaaa atcattcagt gaatcttcta caaaaggata
     6901 agaaaagatc ccgatcccaa cgactaaaat gattgccatg aagatgtcca gtattcttat
     6961 tctgctttta ttcatcatgt ccacctattt tctaatatgt atatgaagag gttgtgaaat
     7021 cagcgccttg acctgagtat tagcgaagca aacggcaaaa tagttcctcc tattttgccg
     7081 tttgttgagc gaataccaag agaccgacca cttgtcacaa cctttctccc catttgaatc
     7141 actatcgttt atttatcgta tgatttttgt ttccaatagt ttaagcttct gtttcttgat
     7201 ggcgtttttt gaaatagatc actgctcctc caactaaagc agtacctacc aagatgaata
     7261 cgatgatccc cataccacca gttgaaggca agattccttt aggttggtta tcgatcgttg
     7321 tttcatcaga agttgttgga tcatcatcta ctgtaaattc agtgtacgca gcttcattct
     7381 taggtaatgc gtagccttct ggtgcttttg tttcttgaag gcagtatgcc ccttgtttca
     7441 gcccttcgat tgaaaatttc ccttctgaat cagaaatcaa tttcacttct tcccagtcag
     7501 aacctgtgac ttcatctgac caagcatatg ggtattctcc tgaggcaagg tttgctattt
     7561 ttttcattaa tttaacagtt ggatcttttg ctcttagaat agaaaattct gcatcggcta
     7621 atttttcttg cgtgttcaca tcttgtttga tgaagtggaa atcaccgtag ttttggacat
     7681 tttctacagt aatcgtgtta tccgtagttg tattttcccg attgactaca ccgtttggat
     7741 tcccaagatt ggcttcatct gcgccatgga tgataccggt tgtttgttgc aattttccgt
     7801 tttttactat aaaagtagtt tctttttgtg gattggttaa ttgataacca tctggtgctt
     7861 ttgtttcaac taaagtataa ttgccgtcta ttaatccatt gacactgatg gatccttcag
     7921 tatcagttgt gaatgtcaac gctgactcag caggatcata gagagcgaca ccagtgtttt
     7981 catcaaaacc ggtcacgaat tgattctctg aatttttgat ctggaattgc gcccctgcta
     8041 acttatctac ccctgaaaag ttcccagctt tctggatttt gatattgctt tctttgatga
     8101 catttttagc atataggtga atcgtgtcta aaggtttgtc agggtctagg gtaagcttaa
     8161 cacgatattt ggcgacgttt cgacaccggg ctttggtgtt tctacaaaga cgtatacagc
     8221 atctttccca tcagatttac ctggaagagt caggttagct aatccttcac cagaagtcgt
     8281 tacacttgcg aagggtgtag cgtctgctgg aactgctgtg tctttacttg gatagtacgt
     8341 agcagtgatg tcatatacgt tgaattcaac gccgttcaac ggttcgctat tcggaaatgt
     8401 caattccgtt ccgttgttta tttgattttc tggtgctgaa gtgaatttca atttatgcaa
     8461 attaaccgta acattttctg gtgtaggtga tacggctgca gaaacctctg cagttcctag
     8521 gccgagaacg aaaggaagca gcatgatagc agttcctaaa aacgtccata tttttgtttt
     8581 gatgtttttc attttatttc cctcctattg tttccttttt gttgtttttt ataaaaccaa
     8641 gctgtccctg ttgaaaggac tagcaataac cctaaaattg agaaacctcc aagcatctct
     8701 tcgcttgttt ttggaaagct tcctccattt ttttcatttg atggaccatt tgtctgcatc
     8761 tgaactggcg tttttccatt attcgtcgga tttgcttttg gttcgttgat gatttccaat
     8821 ggtataggat gaagcggatc ataactgcct ttttcaactt caaagagaat ggttgattga
     8881 ctcaatatat atcctttagg cggtttaatc tccactagtt cgtaacttcc aaaagacaat
     8941 ccatggacag aaaatgttcc atctttactg gaagtaaaaa tcgttgctct tgaatcatct
     9001 ttggctaccc aatgatttat cccgttttct tgcgctaagt attggtcatt ttttttgact
     9061 agaaattgag catctgctag tttttgtttc gtttgccgat ccactttgac aaattgtttg
     9121 cctcctgttt ggatggcaag atgtgtttct actgtttgat tgtcagtcgt caaacgtaca
     9181 tcgttatcaa atgtcgtttt ttcactgttt tctttcaatg tcatttggta acggatcgtt
     9241 aatttttttc cagcaaacga actaagatct tttccagaat ctgataagac aaaaccatgt
     9301 tttatttttt gtgtgtgaaa tgtatgaatc ttgtctccac caatcaaaaa atcgatcgtt
     9361 tctggttcta gccataactg aggatcagct gtatctttga tcgtataggt tcctatctcg
     9421 ttgatgttct caggaattgt tacggtgatg agataaggga ctttatcccc atctgcaaaa
     9481 tctgtctgct tattctctac tgtttttgtt aatgtgagtg cagcaggtat tttttgttcg
     9541 ctctttggat acaagtggat ggtcgtcaat ttatgattag atgaatctgt cacaggaagc
     9601 acaacgacaa atggtgctga tttttctttt acttaatctg aagtctttat ttccgtaaat
     9661 cggtaaacag cgtcacgacc ttgctcatct ttgtccgatg cactaaaaga agcaatgccc
     9721 tcttcctcgt ctactgtttt tgtcacattt tctgccagta ttttttcact atcacttttt
     9781 tgtgctagtt ttctttgtgc gtcttccaca ctacttcctt ctgaccgcag cttgtaaaat
     9841 tcatccgaga catcatatat agcgaaagta acgccgttca acccttttac tccttgaaag
     9901 ttatccggga tatttattcc gtcgttttga ctgatacttg gcatcttatc gctttcgaac
     9961 attcgattat gcaaagtaaa tgtaataggt gattctctag ctgacacagt cggagaacct
    10021 ataaaaaaga ccagtaggat aaaaaagaga acagcaaata tatccacacg tacatttttt
    10081 gtttttttca tcgctgttca cctgcttttt tcttacgatg gataaaataa aagaagccag
    10141 aaaaaataaa gatcagtcct aaaaagacaa agggcagtag acctatccca cctgtcgaag
    10201 gaagcaagag agtcggattg atcgtcaact tagcggtatc tgttgcgata atcgtataat
    10261 tattgtttac acttccttcg cctaaactaa tcgttactat ttgtgatcca atatctgtcc
    10321 ggctgttaac tgttagggta taatccaatg attcgcctaa gacaacaccg gtaacgtgga
    10381 gatttccaac cgaaatcgag tgactttggg catcataatt aaaggcagcg tcatcgacag
    10441 taatcactgc gttagcttta tagatggtat aagttcctga tgtgttggct atatcagtaa
    10501 atgacaactt gggatttgct gctttgactt tgtctattcc gctattcgtt agtttaattg
    10561 tatagctgcc agcattaacc atattttttg ctgattgtgc atctgctgtg taatccatac
    10621 tgctaagtgc cacttgtact ggagtaagcg aactagtgac accctctgct agcttgacat
    10681 ggattgacgg gacacctgct tttccatcat atgtcttact gccactgagc tctaccgcac
    10741 cgctgatttg cttaatcgta tagacatcag atgtctgcac gatagtaaat aaatagtttg
    10801 ttctggcagc tagtttagct agtccgcttt ttgtcaattg ccaagacgta taggttccaa
    10861 tttcagtagg cgcagttact tcaactccct tttcattcat cgctttgaaa tcagttggat
    10921 tccatgtgat ttgatcaata ctgagatcat tgtttaaaag ggatgttgat tctgttttat
    10981 cattaaaggc atatttaacg atcggtacaa cagaaagacc atcatagtac ttactgccat
    11041 ttgcattgat tgatactgtt gcatttgtct tataatatag gatgacttca gaaatctgtt
    11101 ttccatttgc gtcctttacg attacatttc catttgtatc taaactagct aaaacactca
    11161 ttgctgattg gttctttgtt tctatcgctg aaacagctgt aaaggtatat ccgttgatag
    11221 taggcgtatt agtcgtcagg tcttgtgcag cacttccgta agtaagattc attattttag
    11281 aaggagcaat catctgtcca aaatagtctt gatacttaat agtgattggt gtttgtttgc
    11341 ctgtataggt caccgtatcg acgttacttt ctagggctgt gaaagaaccg tttgtttgtg
    11401 caaaagaata gcccgttact gctgttggag tgataaccac tttgtcaccc acatggattc
    11461 cagataatgt agtcccgtac gttccgattt ttgctgctcc accattgact gtataagaaa
    11521 aagttcctgt ccgattcgca ttgacctgaa ccgttgctgc ttgataggtt ggaaaatttg
    11581 aaacatttcc cttaccatca acgatctgcc catttttgat cgagtacccc gtgaatggaa
    11641 tatcatttgc taactgtata ccgattgaaa aagtactcat acctcctccg gcaggaagcc
    11701 ctccccatgt tgagggtgta aaagaataac ttttatccgc attgggtgta actgaatcga
    11761 tcgtatttcc agcggtagcc acccccatat agatactttt tgggaatgtg atagtagtac
    11821 catttactgt ggagccaata aacagattat ggatatctgg acggttatca ataaaagcga
    11881 ttgctttggt tacgccttgt aacgcagttt tatccgcact catcgatccg ttaaccgcaa
    11941 caatgactcc atctttggca tatatcactg tatatccatc tggtatggta tgtccgttga
    12001 aattcgcagt ggccgcaaca gtactaaatg tctggcttct acctgtttct gaataagctt
    12061 tctcccctgc tgcttttgct gctgtgattt ctttttctgt cggatcaatc agttgagtcg
    12121 aggatgtggt tgaggttgtc tcgttgttgc tagactgatt ggtgctttct atggactgta
    12181 tttcagaata gggtggacta tttgtctcct ctgtcgtttg gttagcaaaa tttgacgttt
    12241 ctgtataaac gtttacataa atacttgata agctccctaa gaaagtaaaa atcaagatga
    12301 tcgaaatcat aatgggatgt aagtattttc ttttagatac agctttgagt ctagaaacaa
    12361 aatgtttttg acatatacgt tggattttat tttgagtttt cttaatcatt ttcatccctc
    12421 ctctcaataa a
//
LOCUS       DASHHB010000074        12143 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.077, whole genome shotgun sequence.
ACCESSION   DASHHB010000074 DASHHB010000000
VERSION     DASHHB010000074.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 12143)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12143)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12143
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.077"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..565
                     /locus_tag="U2K71_002436"
     CDS             <1..565
                     /locus_tag="U2K71_002436"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HEM7316462.1"
                     /translation="YFKSEKDTSSLTLTLEEGVEPDKVTSKVINQLKEKGSLKHLGEY
                     EVLDTAMLTKGIGQILSTITYFITAVAGISLFIAGVGVMNMMYISVSERTKEIGIRRA
                     LGATRKSIMLQFLLEGLILTLSGGLIGYLLGMALAYSVGSLIKVNVSLDLFTILLAVG
                     VSSVIGLVFSVMPASEAAKKDLIDILR"
     gene            966..1571
                     /locus_tag="U2K71_002437"
     CDS             966..1571
                     /locus_tag="U2K71_002437"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell filamentation protein"
                     /protein_id="HEM7316463.1"
                     /translation="MILENKLGLTNQVELAKVEEKLSKQKAKELYDCGKINEIEVGTF
                     KGLSEIHEFLFSDIYDFAGKIRSVNIAKGNFRFAPVMYLEHSLQHIDQMPQTSFEEII
                     KKYVEMNIAHPFREGNGRSTRIWLDLILKEELQKVVDWNLIDKADYLSAMERSPINDL
                     EIRYLISNALTDKIDDRELYMKGIDVSYFYEGYSEYTIDDL"
     gene            1587..2159
                     /locus_tag="U2K71_002438"
     CDS             1587..2159
                     /locus_tag="U2K71_002438"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000170424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7316464.1"
                     /translation="MTKYGYARVSTASQSTKEQIQQLLENGVEKKNIFSEKFTGTKRD
                     RPKFNQLLAGLKPGDEIVVTKLDRFARNTREALEIIEPLLDDNVTIKVLNLGTIENTS
                     MGRMVTRTLLSVAEMERDMIVERTQEGKMFARKNNPNFREGRPKATITPKKRHAYELL
                     TSGKSYKEVEAITGFSRSTLFRIKKKIEES"
     gene            complement(2411..2668)
                     /locus_tag="U2K71_002439"
     CDS             complement(2411..2668)
                     /locus_tag="U2K71_002439"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316465.1"
                     /translation="MANLYIENKGDLNAIITLIVVMVKSIGNITIGMLFIILAAVLIF
                     MIVIWFLNERSKDNQHRRDMEIKSIESIDYLSIITLKKPRS"
     gene            complement(2794..3609)
                     /locus_tag="U2K71_002440"
     CDS             complement(2794..3609)
                     /locus_tag="U2K71_002440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316466.1"
                     /translation="MVKIAGLSKGGKTVSQLYKQNYRDWRQFRKNIKDSYFILPTELE
                     KYLPNNKAINLYLYYCFKAKNEGGDSWHSVNTIAESLGVTTKTINTWNKTLVDLGMIA
                     RIDDNHLSKSTILLPLSSYYKLALDSSLKDVTESTVHEIDGNLVAVYHLFEWRKNEAD
                     ENYNVPYNTLCCVFKRKTQKDTIYKFILVQDEKISNFELSTPSSKIYDDAYRFECQDL
                     EALLSANNLNNIEHENFVVNTRFNLVSEKDSDLLDLLIELTKNLDKKENITVL"
     gene            4126..5451
                     /locus_tag="U2K71_002441"
     CDS             4126..5451
                     /locus_tag="U2K71_002441"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEM7316467.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRA
                     DNTGSGLILASSPAAKKKYGISNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTRSMNLFTTEGTRSQRRKKLAQMIQENIKEELGLLA
                     TVGVGDNPLLAKLALDNEAKHNDGFLAEWTYQNVPDKVWNIPEMTDLWGIGSRMKKRL
                     NQIGIMSIKDLANWNPYTIKSHLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLYIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGAGGLKND"
     gene            5444..5794
                     /locus_tag="U2K71_002442"
     CDS             5444..5794
                     /locus_tag="U2K71_002442"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316468.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEKIDADNEQRANQNLAKEQMTTE
                     EVAEVLNAAVIKNRSVCIQKEERNAEGYYPEDIVSKILGSDELGIYVGKNKVHYDEIR
                     HIEFYQDNKWGRLE"
     gene            5776..5961
                     /locus_tag="U2K71_002443"
     CDS             5776..5961
                     /locus_tag="U2K71_002443"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316469.1"
                     /translation="MGSIRMNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSF
                     YVEAAAGYHKYEYYIGY"
     gene            complement(6865..7140)
                     /locus_tag="U2K71_002444"
     CDS             complement(6865..7140)
                     /locus_tag="U2K71_002444"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001196543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgO"
                     /protein_id="HEM7316470.1"
                     /translation="MALLPTNKDKKKIGAEDISIKDDINVELGERKYTAKERKPVQVD
                     PPVLKTIRDLSYAKDIPMYEIVKLAMDAYVDTLNDEERIIYNRRSKD"
     gene            complement(7112..8065)
                     /locus_tag="U2K71_002445"
     CDS             complement(7112..8065)
                     /locus_tag="U2K71_002445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM7316471.1"
                     /translation="MENLRTAFKTSDKLGKVVMLTGRKGGIGKTTDNDLLAIVSSQLF
                     EKDVLLIDYDQQRNTTSNIGSTYQITSFDRSMSAAIKKGDWVSGITQVSPHLYIMAGS
                     PGSEELNEYLSEKYPDRRKRSLAFIKPLEELRKNFDYIFIDCPPSTDNVVRAFLTAAD
                     YIIAMQELKRYAMEGTEDFINKVLVPIVTNFEESHLQIIGILPVLFSVRRSSQHANYQ
                     KTIDKYGESNIFKSIVKGSDRLEMYGENGIQLNDYVDRRWWAIFADIFTELEERIEYY
                     EQHGDIEGFDYTPKYADSMANHILPLGKEIKLNGIITNEQR"
     gene            8677..10170
                     /locus_tag="U2K71_002446"
     CDS             8677..10170
                     /locus_tag="U2K71_002446"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000947690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="primase C-terminal domain-containing protein"
                     /protein_id="HEM7316472.1"
                     /translation="MLSEIYSAILKNGLRTYKYRNSTMKPAGHIDSSRSGAIFAYRSK
                     QLMNVGRGMVITSEEAILENEKKLTHWTPNTYRFGTYADDYRTVVKGHSEKNLSQINT
                     FVVDIDSKENHQGEIILACLDQVGYMPTLILESDHGYQVYFVLKTPGYVTKKSNFKVI
                     DVAKKISKTIRLQLAEKLSGVDLGCNHFGIARFPQENNIVFKEMNYQYSFADWIQWSM
                     KLESDQKTKEDRERKLIVFPEKKEIRQVDEPWFDMLLHKANIVGGEGRLGRNNVIFTL
                     SLAYYSSGYALDTCEYNMFTFNERLQEPLSEAELVKIVRSAYSGNYQAASREFIIELC
                     QQWVSPTLKNNDLFIQRRGWWKFKKPRDQRKYSHKNEWKEDLLAYLSEKSLASEPYLN
                     LSKKELASQLTMPKRTLDKILNELREENKVFYKVTRGRNGGMIIASVKTLFARVIQLS
                     KEKRLDYSTRITEVFGLNESLVEETFRQLSTPLNPLKEVNLFEVDTG"
     gene            10305..10601
                     /locus_tag="U2K71_002447"
     CDS             10305..10601
                     /locus_tag="U2K71_002447"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000053907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEM7316473.1"
                     /translation="MSLKNYVYSHPVNVFIIGRLGLSVEVFCELYGFKQGTLSSWVTR
                     EKTVASLPIEFLHGLSLASGETMDQVYDCLCVLEQEYIEYKIANELRKRKKYIQ"
     gene            10702..11553
                     /locus_tag="U2K71_002448"
     CDS             10702..11553
                     /locus_tag="U2K71_002448"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316474.1"
                     /translation="MKSDDWIEHTWLPLKRNSKRKKFWVLQLWPLGKLCSYGARLILD
                     LTVYSIGVFGTAYYLINDPNRIAASDIFTVLMTLIFVMLLSIFTQLVMQFTRQKLTAG
                     KLFKSLFDLIVVNAILATLIKQIEQSGRTTKGVKDFLLTFIHFVNGHMYLFEVGIILF
                     FVGYVSTKLIKRSIEKEIEIDLIPTLLGYEYNVDFGRYDELINTDIVHTSIHESTVKG
                     IRSILKNGQEMKAIDRRKEIYNFYEVEASLRYGIGKLWLDGSNIRQNKPYIFWNSSDL
                     ELPMKDK"
     gene            11574..11857
                     /locus_tag="U2K71_002449"
                     /pseudo
     CDS             11574..11857
                     /locus_tag="U2K71_002449"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345737.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         4170 a         1864 c         2268 g         3841 t
ORIGIN      
        1 ttattttaag tctgaaaagg atacatcgtc tctaacgtta actttagagg aaggagttga
       61 acctgacaag gtgacctcta aagtgataaa ccaattgaaa gaaaaaggta gtttgaaaca
      121 tttgggggaa tatgaagtgt tagatacggc tatgttaacg aaaggaattg gtcaaatcct
      181 cagtacgatc acttatttta taacagctgt agcaggtata tctttattta tcgcaggggt
      241 aggtgtaatg aacatgatgt atatatctgt atctgaacga acaaaagaga tcggtataag
      301 aagagcccta ggagctactc gaaaatcgat tatgctgcag ttccttttag aaggtttgat
      361 tttaactctt tcaggagggt taattggcta tttattagga atggctcttg cttatagtgt
      421 tggttctttg ataaaggtaa acgtgtcact tgatctattt actattttac tagcggttgg
      481 ggtatcttcg gttattggac tggtcttttc tgtaatgccc gcttcagagg ctgcaaaaaa
      541 agatttaatt gatattctta gataaaatca gaatgacaag aatatttacg ttctagattc
      601 taaaataaaa cacaaagaga ccaaccaggc accctattgt ttctttgtag tatagagata
      661 ttctttggct tgtaagagat caatagagtt aggtgaccgg agttcgaaat aaaggaaatt
      721 tttgattgta taggagaatt ctttatccct aatatttaga attttgagta tgccatgttg
      781 atgttttaga ttctctataa atatagaggt gtatcaaagg tcaactagac tgtgcaaaat
      841 agagacactt gaaaaatcta tcgggggtca gaaacttaat agtttttgac acataaacaa
      901 aatcgcgata taatggggtt gagagtgttt cataaactta tgtcataaag ttaaggagat
      961 ttcgcatgat tttagagaat aaattgggat taaccaatca agttgagttg gctaaggttg
     1021 aagaaaaact cagtaaacaa aaagcgaaag aattgtacga ttgtggaaaa ataaatgaaa
     1081 tcgaagttgg tacatttaaa ggtttatctg aaattcatga atttctattt tcggatattt
     1141 acgattttgc cggaaaaatc cgatcggtca atattgcaaa aggaaatttt cgatttgcac
     1201 cagttatgta tttagaacat tcgttacagc atattgatca gatgccacaa acttcttttg
     1261 aagagattat caagaaatat gtggagatga atatcgctca cccatttcga gaaggaaatg
     1321 gccgaagtac tagaatttgg ctggatctca ttttaaagga agaattgcaa aaagttgtag
     1381 attggaactt aatcgataaa gcagactatc tttctgcaat ggagcgaagt cccataaacg
     1441 atttggaaat acgctatcta atttccaatg ctctgactga taaaattgac gatcgcgagt
     1501 tatatatgaa aggaatcgat gtcagctatt tctatgaagg gtattctgag tatacgatag
     1561 atgatttgta aaagtaggtg gaccaaatga cgaaatatgg atatgcacgt gtctcaaccg
     1621 cttctcaaag tacaaaagaa caaattcaac agttgcttga gaacggcgta gagaagaaga
     1681 atattttttc agaaaaattt acgggtacaa aacgggatcg gccaaaattc aatcagctgt
     1741 tggctggtct gaagcctgga gatgaaatag ttgttacgaa gcttgatcga tttgctcgta
     1801 acacgagaga agcattagag atcattgaac ctttactcga tgataacgta acgatcaagg
     1861 ttttgaatct aggaacgatt gaaaatacgt ccatggggcg aatggtgaca cggacgttgt
     1921 tgagtgtagc agaaatggaa cgtgatatga ttgtggaacg tacccaagaa ggaaaaatgt
     1981 ttgcaagaaa gaataatcct aatttcagag aaggacgtcc gaaagcaaca atcacaccga
     2041 agaagaggca cgcatatgaa ctgcttactt caggtaaatc gtataaggaa gttgaagcta
     2101 taaccggttt cagtcgcagt acgcttttta gaataaagaa gaaaattgaa gaaagttaaa
     2161 aaagaagtct cagcgaacca gacaggtaaa tacttaatgc gttctagata tgagtctatt
     2221 agcatgttgt gaaatataat atttttttat ttacttgatt tctgctcaaa aaatgtgtat
     2281 ggtaactgta aaaggtaaag taattaaagg tatgctagcc acgcttacta tattaggata
     2341 ccatcatata tttataatga acgatttgtt cgtctaccaa acaacacata tcgttcagcg
     2401 taagtcacgt ttaggaacgt ggctttttta gtgttattat cgataaataa tctattgact
     2461 ctattgattt tatctccata tccctacggt gttgattgtc tttagatctt tcatttaaga
     2521 accaaataac tatcataaaa atcagcactg ctgctaatat aataaacagc attcctattg
     2581 tgatattacc tattgatttt accataacta cgattaaagt aataatggca ttcaagtctc
     2641 ctttgttctc tatatataaa ttagccataa atcatcttcc tttcaaatta agatacttgt
     2701 ttgataccaa taaatagtaa gcgtggctag catacctttt tctgtaatag caagtgttct
     2761 aatagtccaa acttaatgat ggaccgatgc tagttatagt acagtaatgt tttctttctt
     2821 atctagattt ttagtaagct ctatcagcaa atctaacaaa tctgagtcct tttcagaaac
     2881 taaattaaat ctagtgttta caacaaagtt ttcatgttct atattgttta gattatttgc
     2941 ggaaaggagg gcttctagat cttgacattc aaatctatat gcatcgtcat aaatcttact
     3001 agaaggagtg cttaattcga aattacttat tttctcatct tggaccaaga taaatttata
     3061 gatagtatct ttttgtgttt ttcttttaaa aacacaacag agtgtgttat aaggaacgtt
     3121 ataattttca tcagcctcat tttttctcca ttcaaacaaa tgataaactg caaccaagtt
     3181 gccatcaatt tcatggacag tgctctcggt aacatccttt aaagaactat caagggctaa
     3241 tttatagtaa ctagagagag gaagtaaaat tgtcgattta ctgagatggt tatcgtctat
     3301 acgtgcaatc atacctaaat caactaaggt tttattccag gtgtttatag ttttagttgt
     3361 cactcctaga gactctgcta tggtattgac cgaatgccag gaatccccgc cttcattctt
     3421 tgccttaaaa cagtaataca gatataaatt aatagctttg ttattcggta aatatttttc
     3481 aagttctgtt ggtagaataa agtaagaatc cttaatgttt tttctgaact gtctccagtc
     3541 tctgtagttt tgtttataaa gttgtgaaac ggtttttcca cctttagaaa gtcctgctat
     3601 cttaaccaat cttattgctc ctttcttgaa tctgtcatga tcatacagta ttttttactt
     3661 gtagtccagc accttaatat attatcctat gtaattaatt tcgtactaga tagtaattat
     3721 tttttattta attctctagt cacttacaaa taagttatat aaactacgat taataaatta
     3781 ttacaaaaac tattaagaaa acttctacat acaactatta caaaactatt aagaaactga
     3841 atactcctat tttttgtttt taaggaaagt tttttctttt ttgaatttct tatttttttc
     3901 taatataact aagcaaaact ggataatacg acacttattt tcaaattttc acaagactaa
     3961 tagtaaatat ccatttaata aagtgataca atgtgttgat tttacaaatc aacagaagaa
     4021 gtagggaaat aacttaccta tgcttctttt tttgtacatt taatcgattc ttctttacaa
     4081 aaacgaacgt atgtttgtat aatgtggaag aatggagtgt tatttatgat ggattattca
     4141 aaagagccag taaatgatta ctttttgatc gatatgaaaa gcttttacgc aagtgtcgaa
     4201 tgtatagaaa ggaatttaga tccattaacg actgaattag tcgtcatgag tcgagcggat
     4261 aatacaggat cagggttgat tttagcttct tcaccagctg caaagaagaa gtacggtatt
     4321 tctaatgtca gtcgaccacg agatctaccg caaccttttc ctaaaacgtt acatgtcgtt
     4381 ccaccacgca tgaatctcta tattaagcga aatatgcagg taaacaatat tttcagaagg
     4441 tatgtagcag acgaagattt attgatttac tcgatcgatg aatcgatctt aaaagtgacc
     4501 agatcaatga atctttttac gactgaagga acaagaagtc aacggagaaa gaagctggct
     4561 caaatgatcc aggagaatat taaagaagag ctaggattgc ttgctacagt aggtgtcgga
     4621 gataatccct tattagctaa acttgcttta gacaatgaag caaaacataa tgatggattc
     4681 cttgctgaat ggacgtatca aaatgttcct gataaagtat ggaatattcc tgaaatgaca
     4741 gacctttggg gtattggatc tcgaatgaaa aaaagactca accaaattgg tatcatgagt
     4801 attaaagact tagctaattg gaatccatac actattaaga gccatttagg tgtgattggc
     4861 ttacagctgt attttcatgc gaacgggatc gatcgaacag atatagcgat cccaccagaa
     4921 ccgacaaagg aaaaatcata tggtaattca caagtgttac cacgagatta taccaggaga
     4981 aatgaaatcg aactggtggt aaaagaaatg gcggaacaag tagctattcg tatcaggcaa
     5041 cataactgta aaacaggttg tgtccattta tacattggca cgtcgatctt agaaacgaga
     5101 ccaggttttt cacaccagat gaagatccct ataacagata atacaaagga attgcagaat
     5161 tactgtcttt tcttgtttga taagtattat gaaggacaag aagtaaggca cgttggaata
     5221 acctatagca agcttgtgta cactgattcg cttcagctgg atttatttag tgatccacaa
     5281 aagcaaattg acgaagaaaa tttagataaa attattgaca agattcggca aaaatatggt
     5341 tttacgtcga ttgtacatgc ttcttctatg ttagaaggcg ctcgtagtat tactagaagt
     5401 acattggttg gtggccatgc aggcggcgca ggagggctta aaaatgattg attataatga
     5461 tcgtggacgt ttaaagtggg caggttttta tttatctgat catactgaaa aaattgatgc
     5521 cgacaatgaa caacgagcca atcaaaattt ggcaaaagaa caaatgacga ctgaagaggt
     5581 tgcggaagta ttgaacgctg cagtcataaa aaatagatca gtatgtattc aaaaagaaga
     5641 acgaaacgct gaaggatact atccagaaga tattgttagc aagatcttag gtagtgatga
     5701 gctggggatc tacgttggaa aaaacaaagt tcactacgac gaaatacgtc atatcgaatt
     5761 ttaccaggat aataaatggg gtcgattaga atgaacatta tccagcaata tgagctaaag
     5821 tatataacct ttgatcagct atcagaagag atttggggtt atggccagcg tttgataaat
     5881 gaggttggtg ttgaacgctt ctctttctat gtagaagctg cagcaggata ccataaatac
     5941 gaatactata ttggctatta aagtgcaata aaattatatt tcttttatgt tttacatttt
     6001 gtatcaattc tgataaaatt acctataggt aattaaaaaa tataaaaaaa ctagttacaa
     6061 ccttaggacg ttgtaactag cttgaagcat actagagcta actaaaatag cactagaaag
     6121 caatgttaaa cccgtccaac aaacttgatt taacattgag accagcttat tcagctaaat
     6181 atagttgaat aagcttcttt tgttttaaaa aaacaaaagg tgtactacga ataacgcaag
     6241 taaaagaaat actattacaa ccagtaggct acaagtaagt attactaaac attgcgtagt
     6301 tagaaccttt gctctattgt ctaaagccac aaaattagag atcagcttta tgatgctaag
     6361 agcgagctgc atccatgcgg acataggttc ttttgacact attaattctc ctttttagga
     6421 taagggaaat tgctagtagt tttttcatta gcaccatccc ccttcatttg attgagtcta
     6481 gtgctgctgt gatagtttgc tgtttaatat gcttacattc ttattataac ataaatgaaa
     6541 caaacacatg gttagaggtc gtatcaatca aaatagtgtc tcacaaacga tttattactt
     6601 actcctgctg actaacggac tgttcttcag taactgtaac tatatgtttg ttaaaaccac
     6661 cattacctgt agataaaaac caatatattt actatactca ctatattaaa tatatttagt
     6721 atagtaaaaa ctagaaacaa taaaatttgt gaatgttgta tacccccaaa caatacacat
     6781 attttgaagg attaggatta ataaagctaa aaatatagta agtatactaa atatatttac
     6841 tatatctagt atacttacta tattttaatc ttttgatcgt ctattatata taatccgttc
     6901 ctcgtcgttt aaagtatcaa cataggcgtc cattgcaagt ttgactattt catacatagg
     6961 aatatctttt gcataactaa gatcccgaat ggtttttagt acgggaggat ctacttgaac
     7021 tggttttctt tctttcgcag tgtactttcg ttcccctaac tcgacattaa tatcatcttt
     7081 aattgaaata tcttcagcac caattttctt cttatctttg ttcgttggta ataatgccat
     7141 ttagtttaat ctcctttcca agaggtagga tatgattggc cattgagtca gcatatttag
     7201 gtgtataatc aaatccttca atatcaccat gttgttcata gtattcaatt ctttcttcta
     7261 attctgtaaa gatgtctgca aaaatagccc accatcttcg atctacataa tcatttaatt
     7321 ggataccatt ctcaccatac atttctaacc gatccgagcc tttaacaatg cttttaaaaa
     7381 tgtttgattc accatactta tcaattgttt tttgatagtt tgcgtgttga gaggaacgtc
     7441 gcactgagaa aagaacaggc aaaattccaa tgatttgaag atgagactct tcaaaattcg
     7501 tgacaatcgg aactaaaact ttattaataa aatcttctgt tccttccatt gcgtaccgtt
     7561 ttaattcttg cattgcaata atataatctg cagcagttaa gaatgcacga accacattat
     7621 ctgttgatgg tgggcagtcg ataaagatat aatcgaaatt ttttctcaat tcttctaaag
     7681 gtttaatgaa ggccaatgat ctcttacgac gatcaggata tttttcggac aagtattcat
     7741 taagttcttc agatccagga gagccagcca taatatataa atgtggactt acttgagtaa
     7801 tacctgaaac ccagtcccct tttttgattg ctgcagacat ggatcgatca aagctagtaa
     7861 tttgatacgt tgatccaatg ttggaagtag tatttctttg ttggtcataa tcaattagca
     7921 acacgtcttt ttcaaaaagt tgactagaaa caatcgctaa taaatcattg tctgtagtct
     7981 taccaatgcc gccttttcta cctgtaagca ttacaacttt tcctagttta tctgatgttt
     8041 taaatgcagt ccgtaagttt tccatagttc cctccaattt cctatactaa atatatttag
     8101 tatacttcat atacttaata tattcgatat atctaatata ctccaaattg ataagaattc
     8161 agactttata aggaatatag tggatatact aaatatattt gctatattca gtatatcaga
     8221 tatattcaaa atgtcaaaat atatttgtgc gttttgcgtt gacaatttag agtgaaaaga
     8281 ggtaaactta attctataga aaccacagat aattcactat ttacaaagta gcaaaagcag
     8341 tggtactctt taaacagaaa aattacataa aaaaaagaga cggaaataat tgattttagc
     8401 agaatcaatt atttctgaat cacatggtta ggacatgtgt tcacgctcgt ctcaagaata
     8461 tgtaagtagc taccaactac ttatattaaa agttgatcgg ataccttttc cgattgcctt
     8521 tattctacca aaaaaaggcg agcaactcaa cagaagaacg tttatttagt gtacttaaat
     8581 taaatattct taaacactga agagagaaca catactatgg ctgagattaa agctaaactg
     8641 tgttctcttt ttgtttgaga aaggacgtta caatcgatgt tgagcgaaat ttattcagct
     8701 attcttaaaa acggcttacg tacatacaaa tatcgaaaca gcaccatgaa acctgcaggt
     8761 catattgatt ccagtaggag tggtgctatc tttgcgtacc gtagcaagca attaatgaat
     8821 gttgggcgtg gtatggtcat tacttcggaa gaagctattt tagagaacga aaagaaacta
     8881 acgcattgga cacctaatac ctatcgcttt ggtacttatg cggatgatta tcgaacagtg
     8941 gttaaaggtc attctgagaa aaacttaagt caaatcaaca catttgtagt tgatatcgat
     9001 tcaaaagaga accatcaagg cgaaattatt ttagcctgtt tagatcaagt gggctacatg
     9061 ccaacgctaa ttttagaatc agatcatggt tatcaagttt attttgtact aaagacacca
     9121 ggctatgtca caaagaaaag caatttcaag gtgatcgatg tagcaaaaaa aatttcaaaa
     9181 acaattcgtc tacagttggc tgaaaaattg tccggagtgg atctaggatg taaccacttc
     9241 ggaattgctc gattcccaca agaaaataat attgttttta aagaaatgaa ctatcagtat
     9301 tcttttgctg actggattca atggtcgatg aagcttgaat cagatcaaaa aacaaaagaa
     9361 gatcgagaac gaaaactaat tgtctttcct gagaaaaaag aaattcgtca agtagatgaa
     9421 ccgtggttcg atatgttact tcataaagca aacatcgtcg gcggagaagg acgtcttggt
     9481 cgaaataatg tgattttcac gctttcttta gcttattact cttcaggtta tgcattggat
     9541 acttgcgaat acaatatgtt cacattcaac gaacggttac aggaaccgct atctgaagca
     9601 gaattagtaa agattgtacg tagtgcttat tctggtaatt atcaagcagc aagcagagaa
     9661 ttcattattg aattgtgcca gcaatgggtt tcaccaacct taaaaaataa tgatctgttc
     9721 attcaacgac gtggctggtg gaaatttaaa aagccacgag atcaaagaaa atatagtcat
     9781 aaaaatgaat ggaaagaaga tctattggct tatctaagcg aaaaaagtct tgctagtgag
     9841 ccttatttga acctgtcaaa aaaagaattg gcttcacaac taacgatgcc caagcgaaca
     9901 cttgataaga tcttaaatga gttacgagag gagaataaag tcttctacaa ggtaacacga
     9961 ggaagaaatg gcggaatgat cattgcttca gtcaaaacgt tgttcgcgag ggttattcaa
    10021 ctaagtaaag aaaagagact agattattca acaaggatca cagaggtatt tggactcaac
    10081 gagagcttag tagaagagac atttagacaa ttatcaaccc ccttgaaccc tcttaaagaa
    10141 gtgaatcttt ttgaagtaga tacaggttga aaaataaaag gtgcaaattg ccttatacaa
    10201 tatatctatg atacttctgt aggtgttgct ccctttgcct agctacgtgt aggtggtata
    10261 cttataactg cgtatgcagt cttatgtatc atggaggaag ttgtatgtcg ttaaaaaatt
    10321 atgtctattc acatcctgtt aatgtgttta tcattggtcg tttgggatta tctgtggaag
    10381 tgttctgtga gctttatggt tttaagcagg ggactttgtc ttcttgggtt accagggaaa
    10441 aaacagttgc ttctttgccg attgagtttc ttcatgggtt gtccttagct tctggggaga
    10501 ctatggatca ggtatatgat tgtttgtgtg tgttagagca ggagtatatt gagtataaaa
    10561 tagctaacga gttaagaaaa aggaagaagt atatccagta gtagttttta tttacttaaa
    10621 aaattggttc attgagcaac ctatcgctgc taaagtagca aaggaaaacg ttaaaggaaa
    10681 gaatttaggg aagaaatcga aatgaaatcg gacgactgga ttgaacacac ctggcttcct
    10741 ctgaaaagaa atagcaaacg aaagaaattt tgggtattgc aattatggcc attaggaaaa
    10801 ctttgtagtt acggggcacg acttattctt gatttaaccg tttactcgat tggagtattt
    10861 ggaacggctt attacttgat aaatgatccc aataggattg cagcatcaga catttttaca
    10921 gtgctaatga cgttgatttt tgtcatgcta ttgtctattt ttactcaatt agtcatgcaa
    10981 tttaccaggc aaaaattaac agcaggaaaa cttttcaaaa gcctattcga tttaattgtt
    11041 gtaaatgcaa ttctagcgac tttaataaaa caaattgaac aatctggaag aacaacaaaa
    11101 ggagtgaagg attttttatt gacgtttatt cactttgtaa atggacacat gtatttgttt
    11161 gaagtgggta ttattctctt ttttgtcggg tatgtatcca cgaaacttat taaacgttca
    11221 attgaaaaag aaattgaaat tgacctgatt ccgacacttc ttggttatga gtacaatgtt
    11281 gattttggaa gatatgacga attgatcaat acggatatcg ttcacacgag tatccatgaa
    11341 tctactgtta agggcatcag atctatttta aaaaatggcc aggaaatgaa agcaatagat
    11401 cgaagaaaag agatctacaa tttttatgaa gtcgaagcaa gcctacgata tggcatcgga
    11461 aaattgtggt tagatggaag taatattcgt caaaacaaac catacatttt ttggaacagt
    11521 agtgatttag aacttccaat gaaagataaa taattatgga gaatcgtttg aaaatgattc
    11581 ctctatacag gattgtgctt atagttggca tggagttaaa aaccttaaag aaggagcagt
    11641 tattcaaagt gaaagaacgt cataatcagt tactatgtag taaaacgggt acgatgaaca
    11701 ttgaataccg aacagaatat ttagaattaa cgaatcaaac tgattttttg ttgattcgaa
    11761 agcaacaaga gcaagaaaaa aattaatggc tacttatagc caattgattg atgaatggga
    11821 acaattcaag gaatctaaaa ttccagaacg tctctaaaag cctttatttg taaagagcat
    11881 aaggaaacgt gggaacactt tttaaaaaaa gtgttccaat aggctgtgcc tattacgttt
    11941 ctcaaaaata aatgtgggaa caaaatattt aaaaatagga tagtgaaagg atagtgaagg
    12001 atagtaaaag gagggtagta ggatagcaaa aaggataggc tccttttcgc tatccttttt
    12061 aaattggagg aattaatatg gaagtaagaa taagaaatgt ggatgagaaa atcgtaagag
    12121 aaattgatcg aagatgtgac gaa
//
LOCUS       DASHHB010000075        11741 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.079, whole genome shotgun sequence.
ACCESSION   DASHHB010000075 DASHHB010000000
VERSION     DASHHB010000075.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11741)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11741)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11741
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.079"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..936
                     /locus_tag="U2K71_002450"
     CDS             <1..936
                     /locus_tag="U2K71_002450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM7316475.1"
                     /translation="GRWKETLPEISHEINIVNLTSDEKNKGLLDPYVIMKNTKDSESL
                     AIDILTFLTGISSRDGERFPILRKAIRAVTNSEVRGLMKVIEELRVENTPLSTSIADH
                     IESFTDYDFAHLLFSDGYVEQSISLEKQLNIIQVADLVLPDKETSFEEYTTMELLSVA
                     MLIVISTFALDFIHTDRSIFKIVDLDEAWSFLQVAQGKTLSMKLVRAGRAMNAGVYFV
                     TQNTDDLLDEKLKNNLGLKFAFRSTDLNEIKKTLAFFGVDPEDENNQKRLRDLENGQC
                     LISDLYGRVGVIQFHPVFEELLHAFDTRPPVRKEV"
     gene            939..3122
                     /locus_tag="U2K71_002451"
     CDS             939..3122
                     /locus_tag="U2K71_002451"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEM7316476.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3119..4120
                     /locus_tag="U2K71_002452"
     CDS             3119..4120
                     /locus_tag="U2K71_002452"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEM7316477.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4135..5049
                     /locus_tag="U2K71_002453"
     CDS             4135..5049
                     /locus_tag="U2K71_002453"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM7316478.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5294..5410
                     /locus_tag="U2K71_002454"
     CDS             5294..5410
                     /locus_tag="U2K71_002454"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEM7316479.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5426..7345
                     /gene="tet(M)"
                     /locus_tag="U2K71_002455"
     CDS             5426..7345
                     /gene="tet(M)"
                     /locus_tag="U2K71_002455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEM7316480.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQREVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7443..8816
                     /gene="tet(L)"
                     /locus_tag="U2K71_002456"
     CDS             7443..8816
                     /gene="tet(L)"
                     /locus_tag="U2K71_002456"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HEM7316481.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9380..10642
                     /locus_tag="U2K71_002457"
     CDS             9380..10642
                     /locus_tag="U2K71_002457"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM7316482.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10887..>11741
                     /locus_tag="U2K71_002458"
     CDS             10887..>11741
                     /locus_tag="U2K71_002458"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000336291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEM7316483.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKK"
BASE COUNT         3901 a         1945 c         2451 g         3444 t
ORIGIN      
        1 ggcagatgga aagaaacctt gccagagatt tcccatgaaa tcaatatcgt caatctgact
       61 tctgatgaga aaaacaaagg cttacttgac ccttatgtaa ttatgaaaaa taccaaagat
      121 tctgaatcac tggctattga tattttgaca ttccttacgg ggatttcctc tcgtgatggg
      181 gaacgcttcc caatccttag aaaagccatt cgtgcagtaa ccaatagtga agtgcgaggg
      241 ttgatgaaag tgattgaaga attacgggtt gagaatacgc cactaagtac cagcatagcc
      301 gaccatatcg aaagttttac agactatgac tttgcccatc tgctttttag tgatggttat
      361 gtagagcagt ctatcagcct tgaaaaacaa ctgaacatta tacaggttgc cgacttggta
      421 cttcctgaca aggaaacttc ctttgaggaa tataccacaa tggagttact atcggtagca
      481 atgctgattg tcattagtac ctttgcgttg gactttatcc atacagaccg aagtattttc
      541 aagattgtag acttagacga agcatggagc ttcttacagg tggcacaagg gaaaacacta
      601 tctatgaagc tggttcgtgc tggtcgtgct atgaacgctg gggtatattt cgtgacccaa
      661 aatacagatg acctcttaga tgaaaaactg aaaaataacc tcggcttaaa atttgccttt
      721 cgttccactg accttaacga gattaaaaaa acccttgcct tttttggtgt agacccagag
      781 gacgaaaaca atcagaagcg attgcgtgat ttggaaaacg ggcaatgcct tatcagtgat
      841 ttatatggtc gtgtcggtgt gatacagttc caccctgtat ttgaagaact gctccatgcc
      901 tttgatacca gaccacctgt gcgaaaagag gtgtaaatgt gaaaccatca atattaaaca
      961 gaataaaatc aaactggacg ctgaaacgtc taggtaaagt ggcaatgaca gtggctttca
     1021 cacttgtgat tgccattttt cttttagcca tgctgggaac ggtggttcaa gctgcgggct
     1081 tggtagatga tacggtcaat gtggcaaatg aatacagccg atacccactt gaaaactatc
     1141 aactggattt ttatgtggat aatagctggg gctggctacc gtggaactgg tcggacggaa
     1201 ttggaaaaca agtcatgtat ggactatatg ccattaccaa ttttatttgg acaatcagtc
     1261 tttatgtttc caatgcgacg ggttacttag tacaggaagc ctattcctta gacttcattt
     1321 ccgctacagc agattccatt ggcaagaata tgcagaccct tgcaggggtt acgcctagtg
     1381 gactatcaac agagggtttc tatgttggat tccttttact cttgattttg gttcttgggg
     1441 tttatgttgc ctatacagga ctgataaaga gagaaaccac aaaggcaatt catgccctca
     1501 tgaattttgt gctggttttt atcctatcgg cttcctttat tgcctacgct cccgactaca
     1561 ttaaaaaaat caatgacttt tcatcagaca tcagcaacgc tagtttgtca ctgggtacaa
     1621 aaattgtcat gccccattca gatagtcaag gcaaggacag tgtggacttg atacgtgaca
     1681 gcctattttc catacaggtt cagcaaccgt ggctactgct tcaatataac agttcagaca
     1741 ttgaaagtat tggtattgac cgtgtggaaa gcctgctctc aaccagtcca gattccaaca
     1801 atggggaaga cagagaaaaa attgttgcgg aagaaattga agatagaagc aataccaata
     1861 tgacgattac aaagacgatt aaccgtttag gtacagtctt cttcctattt gtcttcaata
     1921 ttgggatttc catatttgta ttcctattaa caggaatcat gattttctcg caggtacttt
     1981 ttatcatcta tgctatgttt ctgcctgtga gctttatttt aagcatgatt ccatcatttg
     2041 atggtatgtc aaaacgagcc ataacaaagc tctttaatac cattttgaca cgagctggaa
     2101 tcacattgat tattacgaca gcatttagta tttcaaccat gctctatacc ttatcggctg
     2161 gttatccgtt ctttttgatt gcttttctac agattgtgac ctttgcagga atctacttta
     2221 agctgggcga tttaatgagt atgttttctc tacagagtaa cgattctcaa agtgtgggaa
     2281 gtcgtatgat gagaaaacct cgtatgctta tgcacgctca catgcaccgt ctacagcgga
     2341 aacttggacg ctccgtgact gctttagggg ctgggtctgc cattgccagt gctacgggta
     2401 aaaaaggaca gtcgggttcg ggaagttctg cgagtacaca agcagatcac tctcgaccaa
     2461 acgggcagga aaaatcaaca ctaggaaagc gtatcggtca aaccatcggt acagtagctg
     2521 ataccaaaga cagaatggta gatactgctg gtaatttgaa agaacaggtt aaggatttgc
     2581 cgaccaatgc aagatatgca gtatatcaag gaaaatctaa agtgacggat aatgtccatg
     2641 atttaaccag aagtatttct caaaccaaag cggataaagc cagtggacgc aagaaacagc
     2701 aggaacaaag acggaaaacc attgcggagc gtcgctctga aatggaacag gtcaaacaga
     2761 aaaaacagcc agcttcttcc gttcatgaaa gaccagccac taagcaagaa caatctcatg
     2821 atggacagac ttcaaggcaa acaactgtac aggcttccta tagggaatct caacaagcca
     2881 aacaagagcg tccaacagtt aagtccgatt cttcaagttt aaaaacggaa cgtcaaagta
     2941 atacagttca agaaagaacg gttcaaaagc cagtaacttc aactgcacca acagatagag
     3001 cttcacaacg ttcaatcaca aaagaacgtc agtctactgt tcaaagagta ccactacaaa
     3061 atacaaaaag tagaccacca atcaaaaccg ccaccattaa gaaaggcagt aagaaaccat
     3121 gaagctgaaa actttagtga ttggtggttc gggattattc ttgatggtct tctcactgct
     3181 tctgtttgtc gccattttat tttcagatga acaagatggc ggtttttcca acattcacta
     3241 tggcggtgtg gatgtttccg cagaagtgct ggctcataag cctatggtag aaaaagttgc
     3301 caaagaatat ggaattgaag aatacgtcaa tatacttctt gcgattatac aggtggaatc
     3361 gggtggtaca gcagaagatg ttatgcagtc ctcggaatcc ctcggtcttc cacctaattc
     3421 attgagtaca gaagaatcca ttaagcaagg tgtgaagtat ttcagtgagt tattagccag
     3481 tagcgaaagg cttggtgtag atttagaatc ggttatccag tcctacaatt atggtggtgg
     3541 tttcttagga tatgtggcta atcgtgggaa taaatatacc tttgaactgg ctcaaagttt
     3601 ctcaaaagag tattcgggtg acgaaaaagt gtcttacccc aatcccatag ccatacctat
     3661 caatgggggc tggcgataca attacgggaa tatgttttat gtgcaactgg taacgcagta
     3721 tcttgtcaca acagagtttg atgatgatac agtacaagcc atcatggacg aagctctgaa
     3781 atttgaaggc tggaagtatg tctatggcgg agcttccccg actacctctt ttgattgtag
     3841 cggactgaca caatggacgt atggaaaagc tggaatcaac ttaccacgaa cagcacaaca
     3901 gcaatatgat gtaacccggc atatcccatt attggaagca aaagccggcg atttggtttt
     3961 ctttcattct acctataacg ctggctctta tattactcat atcgggattt atttaggcga
     4021 taaccgtatg tttcatgcag gcgaccctat cggttatgcc gacttaacaa gctcttactg
     4081 gcaacagcat ttagtgggag caggacgaat caaacaataa gaaaggacga ttcaatgatg
     4141 aaattcggaa aaaatcagaa tacagaaaaa cagacaccaa aggaaaagaa acctcgtgtc
     4201 tataaggtca atcctcgtaa aaaggttgtg attgtcctat gggtactttt aggacttagt
     4261 ttcagctttg cgatattcaa gcactttaca gccattgata cccataccat tcatgaaacc
     4321 accatcatag aaaaggaata tgtcgatact catcatgtgg aaaattttgt agagaacttt
     4381 gcgaaagtct actattcatg ggaactaaac gacaagtcca ttgataatcg catagaaaac
     4441 ctgaaagcat atctgacaga ggaacttcaa gctctgaatg tcgatacagt tcgtaaagat
     4501 attcctgtat cgtcttctgt aaaaggattt cagatatgga cggtagaagc agatggcgac
     4561 aatcaatttg atgtaaccta cagtgtagac caactcatta cagaggggga aaatacaaag
     4621 accgtccact ctgcttatat agtgagtgtc tatgtagaca gtactggaaa tatggtcctc
     4681 attaagaatc cgaccattac tagcatacca aagaaatcag actataaacc aaaagctatt
     4741 gaaagtgagg gtacggttga ttccattaca accaatgaaa tcaatgagtt tttaacgacg
     4801 ttcttcaagc tctatcctac agcgacagcc agtgaacttt cctactatgt gaatgacggg
     4861 atattaaaac caatcggaaa agagtacatc tttcaagaac tggtaaatcc tatttacaat
     4921 cgtaaggata atcaagtcac ggtatcgctg acagtggagt atatcgacca gcagaccaaa
     4981 gcaacgcagg tatctcaatt tgatttggta cttgaaaaga acgggagtaa ttggaagatt
     5041 atagaataac aaatattggt acattattac agctattttg taatcacgta ctctctttga
     5101 taaaaaattg gagattcctt tacaaatatg ctcttacgtg ctattattta agtatctatt
     5161 taaaaggagt taataaatat gcggcaaggt attcttaaat aaactgtcaa tttgatagtg
     5221 ggaacaaata attggatgtc cttttttagg agggcttagt tttttgcacc cagtttaaga
     5281 atacctttat catgtgattc taaagtatcc ggagaatatc tgtatgcttt gtatgcctat
     5341 ggttatgcat aaaaatccca gtgataagag tatttatcac tgggattttt atgccctttt
     5401 gggcttttga atggaggaaa atcacatgaa aattattaat attggagttt tagctcatgt
     5461 tgatgcggga aaaactacct taacagaaag cttattatat aacagtggag cgattacaga
     5521 attaggaagc gtggacaaag gtacaacgag gacggataat acgcttttag aacgtcagag
     5581 aggaattaca attcagacag gaataacctc ttttcagtgg gaaaatacga aggtgaacat
     5641 catagacacg ccaggacata tggatttctt agcagaagta tatcgttcat tatcagtttt
     5701 agatggggca attctactga tttctgcaaa agatggcgta caagcacaaa ctcgtatatt
     5761 atttcatgca cttaggaaaa tggggattcc cacaatcttt tttatcaata agattgacca
     5821 aaatggaatt gatttatcaa cggtttatca ggatattaaa gagaaacttt ctgccgaaat
     5881 tgtaatcaaa cagaaggtag aactgtatcc taatatgtgt gtgacgaact ttaccgaatc
     5941 tgaacaatgg gatacggtaa tagagggaaa cgatgacctt ttagagaaat atatgtccgg
     6001 taaatcatta gaagcattgg aactcgaaca agaggaaagc ataagatttc agaattgctc
     6061 cttgtaccct gtttatcatg gaagcgcaaa aagcaacata gggattgagc agcttataga
     6121 agtgataacg aataaatttt attcatcaac atacagaaag aagtctgaac tttgcggaaa
     6181 tgtcttcaaa attgaatatt cggaagaaag acaacgtctt gcatatgtac gcctttatgg
     6241 cggaatcctg catttgcggg attcggttag aatatcggaa aaggaaaaaa taaaaattac
     6301 agaaatgtat acttcaataa atggtgaatt atgtaaaatt gataaggctt attccgggga
     6361 aattgttatt ttgcaaaatg agtttttgaa gctaaatagt gttcttggag atacaaagct
     6421 attgccacag agagagagaa ttgaaaatcc gctccctctg ctgcaaacaa ctgttgaacc
     6481 gagcaaacct caacaaaggg aaatgttact tgatgcactt ttagaaatct ccgacagtga
     6541 cccgcttcta caatattatg tggattctac gacacatgaa attatacttt ctttcttagg
     6601 gaaagtacaa agggaagtga ttagtgcact gttgcaagaa aagtatcatg tggagataga
     6661 actaaaagag cctacagtca tttatatgga gagaccgtta aaaaatgcag aatataccat
     6721 tcacatcgaa gtgccgccaa atcctttctg ggcttccatt ggtttatctg tatcaccgct
     6781 tccgttggga agtggaatgc agtatgagag ctcggtttct cttggatact taaatcaatc
     6841 atttcaaaat gcagttatgg aagggatacg ctatggttgc gaacaaggat tatatggttg
     6901 gaatgtgacg gattgtaaaa tctgttttaa gtatggctta tactatagcc ctgttagtac
     6961 cccagcagat tttcggatgc ttgctcctat tgtattggaa caagtcttaa aaaaagctgg
     7021 aacagaattg ttagagccat atcttagttt taaaatttat gcgccacagg aatatctttc
     7081 acgagcatac aacgatgctc ctaaatattg tgcgaacatc gtagacactc aattgaaaaa
     7141 taatgaggtc attcttagtg gagaaatccc tgctcggtgt attcaagaat atcgtagtga
     7201 tttaactttc tttacaaatg gacgtagtgt ttgtttaaca gagttaaaag ggtaccatgt
     7261 tactaccggt gaacctgttt gccagccccg tcgtccaaat agtcggatag ataaagtacg
     7321 atatatgttc aataaaataa cttagtgtat tttatgttgt tatataaata tggtttcttg
     7381 ttaaataaga tgaaatattt tttaataaag atttgaatta aagtgtaaag gaggaattaa
     7441 ttatgaagtg taatgaatgt aacagggtta aatacaacca aattttaatt tggctttgcg
     7501 ttttatcttt ttttagcgta ttaaatgaaa tggttttgaa cgtctcatta cctgatattg
     7561 caaatgattt taataaacca cctgcgagta caaactgggt gaacacagcc tttatgttaa
     7621 ccttttccat tggaacagct gtatatggaa agctatctga tcaattaggc atcaaaaggt
     7681 tactcctatt tggaattata ataaattgtt tcgggtcggt aattgggttt gttggccatt
     7741 ctttcttttc cttacttatt atggctcgtt ttattcaagg ggctggtgca gctgcatttc
     7801 cagcactcgt aatggttgta gttgcgcgct atattccaaa ggaaaatagg ggtaaagcat
     7861 ttggtcttat tggatcgata gtagccatgg gagaaggagt cggtccagcg attggtggaa
     7921 tgatagccca ttatattcat tggtcctatc ttctactcat tcctatgata acaattatca
     7981 ctgttccgtt tcttatgaaa ttattaaaga aagaagtaag gataaaaggt cattttgata
     8041 tcaaaggaat tatactaatg tctgtaggca ttgtattttt tatgttgttt acaacatcat
     8101 atagcatttc ttttcttatc gttagcgtgc tgtcattcct gatatttgta aaacatatca
     8161 ggaaagtaac agatcctttt gttgatcccg gattagggaa aaatatacct tttatgattg
     8221 gagttctttg tgggggaatt atatttggaa cagtagcagg gtttgtctct atggttcctt
     8281 atatgatgaa agatgttcac cagctaagta ctgccgaaat cggaagtgta attattttcc
     8341 ctggaacaat gagtgtcatt attttcggct acattggtgg gatacttgtt gatagaagag
     8401 gtcctttata cgtgttaaac atcggagtta catttctttc tgttagcttt ttaactgctt
     8461 cctttctttt agaaacaaca tcatggttca tgacaattat aatcgtattt gttttaggtg
     8521 ggctttcgtt caccaaaaca gttatatcaa caattgtttc aagtagcttg aaacagcagg
     8581 aagctggtgc tggaatgagt ttgcttaact ttaccagctt tttatcagag ggaacaggta
     8641 ttgcaattgt aggtggttta ttatccatac ccttacttga tcaaaggttg ttacctatgg
     8701 aagttgatca gtcaacttat ctgtatagta atttgttatt acttttttca ggaatcattg
     8761 tcattagttg gctggttacc ttgaatgtat ataaacattc tcaaagggat ttctaaatcg
     8821 ttaagggatc aactttggga gagagttcaa aattgatcct ttttttataa caggaattca
     8881 aatctttttg ttccattaaa gggcgcgatt gctgaataaa agatacgaga gacctctctt
     8941 gtatcttttt tattttgagt ggttttgtcc gttacactag aaaaccgaaa gacaataaaa
     9001 attttattct tgctgagtct ggctttcggt aagctagaca aaacggacaa aataaaaatt
     9061 ggcaagggtt taaaggtgga gattttttga gtgatcttct caaaaaatac tacctgtccc
     9121 ttgctgattt ttaaacgagc acgagagcaa aacccccctt tgctgaggtg gcagagggca
     9181 ggtttttttg tttctttttt ctcgtaaaaa aaagaaaggt cttaaaggtt ttatggtttt
     9241 ggtcggcact gccgacagcc tcgcagagca cacactttat gaatataaag tatagtgtgt
     9301 tatactttac ttggaagtgg ttgccggaaa gagcgaaaat gcctcacatt tgtgccacct
     9361 aaaaaggagc gatttacata tgagttatgc agtttgtaga atgcaaaaag tgaaatcagc
     9421 tggactaaaa ggcatgcaat ttcataatca aagagagcga aaaagtagaa cgaatgatga
     9481 tattgaccat gagcgaacac gtgaaaatta tgatttgaaa aatgataaaa atattgatta
     9541 caacgaacgt gtcaaagaaa ttattgaatc acaaaaaaca ggtacaagaa aaacgaggaa
     9601 agatgctgtt cttgtaaatg agttgctagt aacatctgac cgagattttt ttgagcaact
     9661 ggatccagga gaacaaaaac gattttttga ggaaagttat aaattatttt ccgaacgata
     9721 tggcaagcaa aatattgctt atgcaacagt tcataatgat gagcaaaccc ctcacatgca
     9781 tttaggtgtt gtgcctatgc gtgatggaaa actgcaagga aaaaatgtgt ttaatcgtca
     9841 agaactgtta tggctacaag ataaattccc cgagcatatg aaaaaacagg gttttgagtt
     9901 gaagcgtggt gaacgtggct ctgaccgtaa acatattgag acagctaaat ttaaaaaaca
     9961 aactttggaa aaagagattg attttctaga aaaaaattta gcagttaaaa aagatgaatg
    10021 gactgcttat agcgataaag ttaaatcaga tttagaagta ccagcgaaac gacacatgaa
    10081 aagtgttgaa gtgccaacgg gtgaaaagtc catgtttggt ttgggaaaag aaataatgaa
    10141 aacagaaaag aaaccaacca aaaatgttgt tatatcggag cgtgattata aaaacttagt
    10201 gactgctgcg agagataacg ataggttaaa acagcatgtt agaaatctca tgagtactga
    10261 tatggcgaga gaatataaaa aattaagtaa agaacatggg caagttaaag aaaaatatag
    10321 tggtcttgta gagcgattta atgaaaatgt aaatgattat aatgagttgc ttgaagaaaa
    10381 caagtcttta aagtctaaaa taagcgattt aaagcgtgat gtgagtttaa tctatgaaag
    10441 cactaaggaa ttccttaagg aacgtacaga cggcttaaaa gcctttaaaa acgtttttaa
    10501 ggggtttgta gacaaggtaa aggataaaac agcacaattc caagaaaaac acgatttaga
    10561 acctaaaaag aacgaatttg aactaactca taaccgagag gtaaaaaaag aacgaagtcg
    10621 agatcaggga atgagtttat aaaataaaaa aagcacctga aaaggtgtct ttttttgatg
    10681 gttttgaact tgttctttct tatcttgata tatatagaaa taacgtcatt tttattttag
    10741 ttgctgaaag gtgcgttgaa gtgttggtat gtatgtgttt taaagtattg aaaaccctta
    10801 aaattggttg cacagaaaaa ccccatctgt taaagttata agtgaccaaa caaataacta
    10861 aatagatggg ggtttctttt aatattatgt gtcctaatag tagcatttat tcagatgaaa
    10921 aatcaagggt tttagtggac aagacaaaaa gtggaaaagt gagaccatgg agagaaaaga
    10981 aaatcgctaa tgttgattac tttgaacttc tgcatattct tgaatttaaa aaggctgaaa
    11041 gagtaaaaga ttgtgctgaa atattagagt ataaacaaaa tcgtgaaaca ggcgaaagaa
    11101 agttgtatcg agtgtggttt tgtaaatcca ggctttgtcc aatgtgcaac tggaggagag
    11161 caatgaaaca tggcattcag tcacaaaagg ttgttgctga agttattaaa caaaagccaa
    11221 cagttcgttg gttgtttctc acattaacag ttaaaaatgt ttatgatggc gaagaattaa
    11281 ataagagttt gtcagatatg gctcaaagat ttcgccgaat gatgcaatat aaaaaaatta
    11341 ataaaaatct tgttggtttt atgcgtgcaa cggaagtgac aataaataat aaagataatt
    11401 cttataatca gcacatgcat gtattggtat gtgtggaacc aacttatttt aagaatacag
    11461 aaaactacgt gaatcaaaaa caatggattc aattttggaa aaaggcaatg aaattagact
    11521 atgatccaaa tgtaaaagtt caaatgattc gaccgaaaaa taaatataaa tcggatatac
    11581 aatcggcaat tgacgaaact gcaaaatatc ctgtaaagga tacggatttt atgaccgatg
    11641 atgaagaaaa gaatttgaaa cgtttgtctg atttggagga aggtttacac cgtaaaaggt
    11701 taatctccta tggtggtttg ttaaaagaaa tacataaaaa a
//
LOCUS       DASHHB010000076        11295 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.080, whole genome shotgun sequence.
ACCESSION   DASHHB010000076 DASHHB010000000
VERSION     DASHHB010000076.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11295)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11295)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11295
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.080"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(17..1246)
                     /gene="hflX"
                     /locus_tag="U2K71_002459"
     CDS             complement(17..1246)
                     /gene="hflX"
                     /locus_tag="U2K71_002459"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HEM7316484.1"
                     /translation="MEEKVIIVGVETEANQRYFSESMEELVQLTNTASGEVVFTITQK
                     RPQVDRQTIIGKGKLQELVQQADAHEADLIIFNHELTPRQSQLITDAVGLPVIDRVQL
                     ILDIFAMRARSKEGKLQVELAQLEYLLPRLVGQGKTLSRLGGGIGTRGPGETKLETDR
                     RHIRNKIFAVKKELKEVEAHRERNRQKRKNSEIFQIGLIGYTNAGKSTILNLLTQADT
                     YSKDQLFATLDPLTKRWRFAEGFEITVTDTVGFIQDLPTQLIDAFHSTLEESQNMDLL
                     LHVVDASSPDRILQEQTVLKLMDELNMKEMPILTVYNKADQIDPAMFTPTLFPNVLIS
                     TQTKEGKMALIEAIKRQLMELMTPYTLSVPSNEGQKLSELRRQTMVLDEAYMEETNEY
                     EVKGFAKTNSKWLRKNQ"
     gene            complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="U2K71_002460"
     CDS             complement(1248..2165)
                     /gene="miaA"
                     /locus_tag="U2K71_002460"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HEM7316485.1"
                     /translation="MEKVLVIVGPTAVGKTALSVELAQLFGGEIISGDSLQIYKNLDI
                     GTAKIKEEEMNNVPHHLIDVIEPTQRYSAADFQHSGRQLITEIANRGHLPIIAGGTGL
                     YIQSLLYDYQLGATEEANPEIRKKYETLAETIGSQALWNQLKEKDPAAAEKIHWNNQR
                     KVIRALEVLETTGHSITAPKEEPKQLYDYLMIGLDTKRSILYERINQRVDLMLAEGLV
                     AEARKVYELGEVQASQGIGYKEFFPYFEGKETLEEATEQVKLHSRRYAKRQLTWFRNR
                     LDAQWFDLISEPNQKSQIEQTIKKWLEAE"
     gene            complement(2169..2906)
                     /locus_tag="U2K71_002461"
     CDS             complement(2169..2906)
                     /locus_tag="U2K71_002461"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEM7316486.1"
                     /translation="MLQIYAHRGSSGTHPENTLPAFAEAVRVGADGIELDVHLSKDGY
                     LIVMHDEEVDRTTNGKGLIREKTLEELKKLNAGGKFSKEFPAAKVPELREVLGLLLQK
                     NYRGMLMIEIKTDQYEYPGIEEKLAETLKSRDWPFQHAYCSFHLDSLEKIALLEPEIQ
                     LDLLMSTSDKKIQLAAETPYIEGVHPKNTWLFEHPVQAADFPKAIRAWTINDEEEMAK
                     CQELKIDGIITDFPEKALRWKQSTKSR"
     gene            complement(2997..3524)
                     /locus_tag="U2K71_002462"
     CDS             complement(2997..3524)
                     /locus_tag="U2K71_002462"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316487.1"
                     /translation="MIVGITGLIGVLTGAVVVYIGMILQMRHLENKEQRRRELELKVK
                     EIETLNQLNKKINEILQKRTTLMEEYVSFDAFDDCYITIDDFAYLQSFSAQNSFYLPN
                     YILDELFKNISHRKVILSPDETMKIGGYAYKGGRVILENFTDSLTEMINEKKAQLKTM
                     TRQPIGFFSKDDVWS"
     gene            3704..4297
                     /gene="clpP"
                     /locus_tag="U2K71_002463"
     CDS             3704..4297
                     /gene="clpP"
                     /locus_tag="U2K71_002463"
                     /EC_number="3.4.21.92"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp endopeptidase proteolytic
                     subunit ClpP"
                     /protein_id="HEM7316488.1"
                     /translation="MNLIPTVIEQSSRGERAYDIYSRLLKDRIIMLSGQVTDDLANSI
                     IAQLLFLDAQDSEKDIYLYINSPGGSVTAGMAIYDTMNFVKADVQTIVMGMAASMGSF
                     LLTAGTKGKRFALPNAEIMIHQPLGGAQGQATEIEIAARHILQTRERLNKILAERTGQ
                     PLEVIEKDTDRDNYMTAEQAKAYGLIDEVMENSSSLN"
     gene            complement(4401..4886)
                     /locus_tag="U2K71_002464"
     CDS             complement(4401..4886)
                     /locus_tag="U2K71_002464"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008267279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma carbonic anhydrase family protein"
                     /protein_id="HEM7316489.1"
                     /translation="MERFIASNATVIGDVTLSEDVTIWYQAVLRGDSNWIKIGQRTNI
                     QDGTIIHVDHDAPVDIAENVTVGHQCMLHGCTIEKGALIGMGTTILNHAVIGENSLIG
                     AGSLVTEGKVIPPNVLAFGRPAKVIRPLTKEEIQKNKENIQHYVEIGRMHAQGKFKEQ
                     R"
     gene            complement(5039..5236)
                     /locus_tag="U2K71_002465"
     CDS             complement(5039..5236)
                     /locus_tag="U2K71_002465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316490.1"
                     /translation="MEQFLNEKITEAKREVDRLTHVCQTKPFDSADFFENEFQIERLA
                     AQIEVYELILKKVIEENIDVQ"
     gene            complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="U2K71_002466"
     CDS             complement(5331..7091)
                     /gene="efrB"
                     /locus_tag="U2K71_002466"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_029485765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrB"
                     /protein_id="HEM7316491.1"
                     /translation="MKKTFSSLKRLARYIRPYRETFLLVILFTVLTVAFNVALPYVTG
                     LPTTEISRNIANGESINFSYIFKCLIWITVVGIGYCISQLLSGVLMTNVVQSAMHDLR
                     RDIEEKINRLPVSYFDKNQQGNILSRVTNDVDAVSGALQQAFIGIVNAILGITLAAAM
                     MFYIQPMMALISMIMIPTSIWISKRVINASQKYFQSMQNSLGELNGYVQENMTGFSVL
                     KVYGREKETLEGFKKVNHSLKYFGFRAAFISGLMMPLVQLTAYATYIGMAVLGSFYVI
                     TGAIVVGQLQAFIQYIWQISQPMGNVTQLSSVLQSASAATKRVFEILDEPEEKENKVD
                     ASLPETILGDVTFDHVDFAYDPKKPLIQDLNFEVKAGQTVAVVGPTGAGKTTLINLLM
                     RFYDVDKGAIRIDGIDTKSMSRSDVRSLFGMVLQDAWLYQGTIADNIRFGKLDATDYE
                     VVDAAKTANVDHFIRTMPDGYDMAINSEGDNVSLGQKQLLTIARAVVSDPKILILDEA
                     TSSVDTRLEALIQKAMDRVMEGRTSFVIAHRLSTIRDADLILVMDQGQIIEKGTHESL
                     LAQGGFYEKLYNSQFAEEAE"
     gene            complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="U2K71_002467"
     CDS             complement(7088..8818)
                     /gene="efrA"
                     /locus_tag="U2K71_002467"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux ABC transporter subunit EfrA"
                     /protein_id="HEM7316492.1"
                     /translation="MKLMWHYTMRYKKFLFFNFICVFGFILIELGLPTILARMIDVGI
                     RNDDFDYVKQQGIFMIVITVIGIILNILLGYFGSRITTNIVADIRDDLFEKIQSYSHQ
                     EYENLGVSSLITRTTNDAYQIMLFLQNILRIGFMAPMMFIVSLYMVMRTSVTLSLYVV
                     GALPLLLLAVVAIAKVSEPLSKKQQKNLDKINSILRENLSGLRVIRAFVNEKFEEKRF
                     AVVNENYASSSKSLFRLMAVAQPGFYFLFNIVMVLIIWSGAVQIDAGELRVGDLIAFI
                     EYIFHALFSFMLFASVFMMYPRAAVSAGRIQEALDAKPSIVEDPNGISDTKTKGYIEF
                     QNVTFAYPGHSQEPVIRNVSFTASPGETVAFIGSTGSGKSTLIQLIPRFYDVSEGKIL
                     IDGVDVRDYRLSKLRDKIGFIPQKALLFTGTIADNLRYGKEDATQEEMERAAEIAQAS
                     EFISRKPDGFNEHLSEGGSNFSGGQKQRLAIARAIIRRPEIYIFDDSFSALDYQTDAK
                     LRARLKKETTESTVLIVAQRVGTIMHADKIIVLNEGEVVGMGTHRELLEKCPIYYDIA
                     ASQLSKEELA"
     gene            9211..9423
                     /locus_tag="U2K71_002468"
     CDS             9211..9423
                     /locus_tag="U2K71_002468"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-dependent RNA polymerase auxiliary subunit
                     epsilon family protein"
                     /protein_id="HEM7316493.1"
                     /translation="MIYKVYYQETKVRNPKREETKSLYIEAKSDVDARQRVEENTPYN
                     IEFVQLLEGSHLEYEKENADFSLVEF"
     gene            9425..11104
                     /locus_tag="U2K71_002469"
     CDS             9425..11104
                     /locus_tag="U2K71_002469"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HEM7316494.1"
                     /translation="MKLSIKNNETGVFAIGGLGEIGKNTYGVQFQDEIILIDAGIKFP
                     EDDLLGIDYVIPDYSYIIQNLHKVKALVITHGHEDHIGGIPYLLKQANIPIYAGPLAL
                     ALITNKLDEHGLLRDAELHEINEDTIIRFRKTSVSFFRTTHSIPDALGIVVKTPSGNI
                     VATGDFKFDFTPVGEPANLHKMARIGEEGVLCLLSDSTNAEIPTFTKSEKTIGHSILK
                     IFSKIEGRIIFASFASNIFRLQQAADAAVATGRKIAVFGRSMENAIVNGQRLGYIKVP
                     DGTFVDAHEINRLPANETMILCTGSQGEAMAALSRIANGTHRQIQIQPDDTVIFSSSP
                     IPGNTTSVNHLINLLSEAGAEVIHGKINNIHTSGHGGQEEQKLMLRLMKPKYFIPVHG
                     EFRMLKVHASLAQDVGVPAENCFIMENGDVLALTSDSARPAGHFVAGDVYIDGSGIGD
                     IGNVVLRDRHLLSEEGLVLAVATVDIKKKEILAGPDILSRGFVYMRESGEMINEAQRI
                     LFHALRECLNGPDCSEYKLKEAMTGALQPFLFERTERHPMILPMVMTPDEE"
BASE COUNT         3275 a         2437 c         1980 g         3603 t
ORIGIN      
        1 ttaaaaaaat aaaattttat tggttttttc ttaaccattt tgaattggtt tttgcaaacc
       61 ctttaacttc atattcgttt gtttcttcca tgtacgcttc gtccaaaacc attgtttgtc
      121 ttctcaactc gcttaatttc tgtccttcgt tgcttggcac agaaagtgta taaggggtca
      181 ttagttccat cagctgacgt ttgattgctt cgatcaacgc catcttacct tctttcgtct
      241 gagtagaaat caatacattt gggaatagcg taggcgtgaa catcgctggg tcgatctggt
      301 ctgccttatt gtaaacagtc agtatcggca tttctttcat attcagttca tccatcaatt
      361 ttaagacagt ctgctcctgt aaaatacgat ctggagaact agcatcgact acatgtagca
      421 aaagatccat attttgactt tcttctaatg tagaatggaa agcatcgatc agttgcgtag
      481 gcaggtcttg gatgaacccg acggtatccg ttacagtaat ctcaaatcct tcagcaaaac
      541 gccatctctt agtcaatgga tctaacgttg caaacaattg atctttcgaa taagtatccg
      601 cttgtgtcaa tagattcaag atagttgact tcccggcatt cgtgtagcca ataagtccaa
      661 tctgaaaaat ctcgctattt tttctttttt ggcgattgcg ctctcgatga gcttctactt
      721 ctttcaattc tttcttaaca gcaaaaattt tattccgtat atgtcggcgg tctgtttcta
      781 actttgtttc acctggccct cttgttccga tcccaccgcc aagacgagat aatgttttcc
      841 cttgccctac taaacgaggc agcaggtatt ctaactgagc aagttcaacc tgcagcttcc
      901 cttctttcga acgagcacgc atcgcaaaaa tatctaagat aagctgcacg cgatcaatta
      961 ccggaagacc aacagcatcg gtaatcagtt ggctctgtct cggagttagc tcatgattaa
     1021 agatgatcaa atccgcttca tgcgcatccg cttgttgaac taattcttgt aatttccctt
     1081 taccgataat cgtttgccga tccacttgtg gtctcttttg tgtaattgtg aatacgactt
     1141 ctcctgatgc ggtattcgtc agctgaacta gctcttccat tgactctgaa aagtaacgct
     1201 ggtttgcttc ggtttctacc cctacgatga tcactttttc ttccatttta ttccgcctcc
     1261 agccattttt tgattgtttg ctcaatctga cttttttgat ttggctcact tataagatca
     1321 aaccactgtg catccaaacg gttacgaaac cacgtcaact gacgtttcgc gtatcttctc
     1381 gaatgtagtt tgacttgttc tgttgcttct tccaatgttt ctttcccttc aaaataaggg
     1441 aaaaattctt tatatccaat accttgactg gcctggactt ctccaagctc atacacctta
     1501 cgcgcttctg ctactaaacc ttctgctagc atcaaatcga ctctttgatt gattcgttca
     1561 taaagaatac tacgcttcgt atctaatcca atcatgaggt aatcatacaa ttgctttggc
     1621 tcttctttag gtgccgtgat actatgtcct gtcgtctcta aaacttccag agctcttatc
     1681 actttgcgtt gattgttcca gtggattttc tcagcagctg ccgggtcttt ttctttcaac
     1741 tgattccata atgcttggga accaattgtt tcagctaacg tttcatactt ttttctaatc
     1801 tcaggattcg cttcttctgt cgctcctaac tgataatcat atagaaggga ttggatatag
     1861 agacccgtac caccagctat gataggtagg tgccctcggt tcgcaatctc ggtaatcaat
     1921 tgtcgacctg aatgctgaaa atcagcggct gagtaacgtt gagtaggttc gatcacatca
     1981 atcaaatgat gcggaacatt attcatctct tcttctttga tttttgctgt accgatatcc
     2041 agatttttat agatttgcaa tgaatcacca ctgatgatct ctcctccaaa tagttgtgcg
     2101 agttccacac ttagcgctgt tttaccgact gctgtcggac cgacgatcac taatactttt
     2161 tccatatttt acctactttt cgttgattgt ttccaccgaa gagctttctc cggaaaatca
     2221 gttattattc catctatttt gagttcttga catttggcca tttcttcttc atcgttaatc
     2281 gtccatgcac gaattgcttt tggaaaatca gctgcctgga ctggatgttc gaacaaccaa
     2341 gtattttttg gatgtactcc ttctatataa ggggtttctg ctgctagttg gatcttttta
     2401 tcagaagtac tcatcaataa gtccagctgt atctccggtt ccagtaatgc gattttttcc
     2461 aaagaatcca aatgaaagct gcaatatgca tgttgaaaag gccaatctct actttttaat
     2521 gtttcggcta atttttcttc gataccggga tattcatact gatcagtttt aatttcaatc
     2581 attagcatcc ctcggtaatt tttttggagg agcagtccta acacttctct caattcagga
     2641 actttagctg ctggaaattc tttgctgaat ttgccccctg cattcaattt tttgagttct
     2701 tccaatgttt tttctcgaat cagcccttta ccgttagtcg tccggtccac ttcttcgtcg
     2761 tgcataacga tcaaataacc atctttgctt agatggacat ctagttcgat accatctgca
     2821 cctactcgta cagcttctgc aaatgcagga agcgtatttt ctgggtgggt gccgctgctt
     2881 ccgcgatggg catagatttg taacatcttt cttcactccg ttcagttcta ttgtatcatt
     2941 aagaaaaaaa gaaagcatta ccacgctctc atgtgcaacg gcaatgcttc aaaatatcag
     3001 ctccaaacat catctttcga gaagaaacca atcggctggc gcgtcatagt cttcaactga
     3061 gctttttttt cattgatcat ttcagtcaga ctatccgtaa aattctctaa aatcacgcgt
     3121 ccgcctttat aagcatatcc accaatcttc attgtttcat ctggagataa aatcactttt
     3181 cgatgggaga tattcttaaa tagctcgtct aaaatataat ttggcagata aaaactattt
     3241 tgcgctgaaa atgattggag atacgcaaaa tcgtcaatcg tgatataaca atcatcaaat
     3301 gcatcaaagc tgacatattc ttccatcagc gttgtccgtt tttgcaagat ctcgttgatc
     3361 tttttattta attgattcag cgtctcgatt tctttgactt tcaattccaa ttcacgtcta
     3421 cgctgttctt tattttccaa gtgacgcatt tgcagtatca tgccgatata gacaactaca
     3481 gcacctgtca gcactccgat aaggccagta ataccaacta tcaatctgtt gttcctcctt
     3541 gttttgtttc tctgcttatc ataccataaa gcgggtttat gcgactaatt tgcctaagtt
     3601 ttttatttga ccttttttga ccaaaaatgt atactacatt ttaggtatta gaaactaaat
     3661 tatggtacac tatttctaaa cgaaatcaag gaggaactct attatgaatt taattcctac
     3721 agtcattgaa caatcttcac gaggtgaaag agcttacgat atctactcac gtttattgaa
     3781 agaccgtatc atcatgttaa gcggccaagt aacagatgat cttgctaatt cgatcatcgc
     3841 ccaattgctt ttcttagacg ctcaagattc tgaaaaagat atctatcttt atatcaattc
     3901 tcctggtggt tcagttacag caggtatggc tatttacgac acaatgaact ttgtcaaagc
     3961 agatgtccaa acaattgtta tgggtatggc tgcttcaatg ggaagcttct tgctgacagc
     4021 tggtacaaaa ggtaaacgat ttgctttacc aaatgctgag atcatgatcc atcaacctct
     4081 tggcggcgct caagggcaag caactgaaat cgaaattgcg gcacgccata ttttgcaaac
     4141 acgcgaacgc ttaaacaaaa ttttagctga acgcactgga caaccgcttg aagtgattga
     4201 aaaagataca gatcgtgaca actatatgac agctgaacaa gcaaaagcat atggattgat
     4261 cgatgaagtc atggaaaaca gttcttcatt aaattaatct gtttcaaagg aagctttttt
     4321 aagctctctt atctaaacca aataaaaaag gatggtcaac tcaaactgac catccttttt
     4381 tattttatgg atgctactgc ctatctttgc tccttgaatt ttccttgcgc atgcatccga
     4441 cctatctcga cataatgctg gatgttttct ttgttttttt gtatttcttc ttttgtcaaa
     4501 ggacggatca ctttagctgg gcgaccaaaa gccaagacat taggaggaat aacttttcct
     4561 tctgttacta atgatccagc accaatcaaa ctattctcac caataaccgc atgattcaat
     4621 atcgtggtcc ccatcccgat cagcgcccct ttttcgattg tacagccatg tagcatgcac
     4681 tgatgtccta ccgtcacatt ctccgcaata tctacaggtg catcatgatc gacatggatg
     4741 attgtaccat cttggatatt cgttctttgt cctattttta tccaattact gtccccgcgt
     4801 aaaacagctt gataccagat cgtcacatct tctgacaatg tcacatcacc aatcactgtg
     4861 gcatttgatg ctataaaccg ttccatattt atttttctcc tttcattttt tgtagaaact
     4921 aatcctattg aataaaggta tcatagcata tatttatttc ataagaaaaa aaacaggtaa
     4981 aacctgctca ggccagatgt ctatttacag tatctggtaa ctgacgtttt acctgttttt
     5041 attgtacatc tatattttct tctattactt tttttaaaat taattcgtac acttctattt
     5101 gtgctgccaa gcgttcgatt tgaaactcat tttcaaaaaa atcagctgaa tcaaaaggct
     5161 tcgtctgaca gacgtgcgtt aaacgatcaa cttctctttt ggcttcagta attttttcat
     5221 ttaaaaactg ttccatctcc gtgtcctcac ttttcttgat aactttactt taccattatc
     5281 aagaatagag aacaaaaaat aagttttaaa ctgttttatt cttatatgac ttattctgct
     5341 tcctcagcaa attggctgtt atacagtttt tcataaaatc caccttgtgc caataaactc
     5401 tcatgcgttc ctttttcaat gatctgacct tgatccatca ctagaatcag atctgcatca
     5461 cggattgtcg ataaacggtg agcgatgacg aaacttgtcc gtccctccat cacgcgatcc
     5521 attgcttttt gaatcaacgc ttctaaacgg gtgtctactg aacttgttgc ttcatctaaa
     5581 atcaagattt taggatcgga gaccactgct cgggcaatcg tcagcaactg tttttgtcct
     5641 aaagacacat tgtctccctc agaattgatg gccatatcat atccatctgg catcgttcgg
     5701 ataaaatgat ctacgtttgc tgttttcgca gcatcaacta cttcataatc tgtcgcatca
     5761 agctttccga agcggatatt atctgcaatc gttccttgat aaagccatgc atcttgcaag
     5821 accataccaa atagcgaacg cacatcactg cgactcattg atttggtgtc gattccatca
     5881 atccttattg ctcctttgtc tacgtcataa aaacgcatca atagattgat caatgtcgtc
     5941 ttgccggcac cggtcggacc gaccacagct acggtttgac cagctttcac ttcaaaattc
     6001 aaatcttgga ttaatggttt cttcggatca tatgcaaaat cgacatgatc gaatgtcaca
     6061 tctcctaaaa tcgtttctgg cagtgaagca tctactttgt tttctttttc ttctggctca
     6121 tctaaaatct cgaatacacg tttcgttgcc gctgatgcac tttgcaaaac tgaagacaat
     6181 tgtgtcacgt tccccattgg ctgactgatt tgccaaatat attggatgaa tgcttgtagt
     6241 tgaccgacaa cgatcgcccc agtaataaca taaaagcttc ctagaacagc cataccaata
     6301 taagtagcat aggcagttaa ttgaactaga ggcatcatca agccagaaat gaatgctgca
     6361 cgaaacccaa agtatttcaa gctgtgatta acttttttga atccttccaa tgtttctttt
     6421 tcgcgtccat atactttcaa tacactaaaa cctgtcatat tttcttggac gtagccgttt
     6481 agctcaccaa gagaattctg catgctttga aaatatttct gcgaagcatt gatcactcgt
     6541 tttgagatcc agatcgatgt tggaatcatg atcatagaga tcagcgccat catcggctgg
     6601 atataaaaca tcattgctgc agccaacgta attcccaata tcgcatttac gataccaata
     6661 aaagcttgtt gtaaagcacc gctgactgca tctacatcat tcgtcacacg agacaaaata
     6721 tttccttgct ggtttttatc gaaatatgaa accggaagac gattgatttt ttcttctatg
     6781 tctcttctca aatcatgcat ggcactttga acgacatttg tcatcaaaac accagaaagt
     6841 aattgcgaaa tacaataccc tattccaaca actgtgatcc aaatgagaca tttgaatata
     6901 taagaaaaat tgattgattc gccattggca atattacggc tgatttctgt agtaggaagt
     6961 cctgtaacat atggcaaagc cacgttgaaa gccactgtca gaactgtaaa taggatcact
     7021 aaaagaaaag tctcacgata cggtctgata tatcgagcta aacgttttaa ggaagaaaag
     7081 gtttttttca tgctaattcc tcctttgata attgagaagc ggcaatatca taataaatcg
     7141 ggcatttttc caacaactcc cgatgcgtcc ccataccgac gacttctcct tcatttaata
     7201 cgatgatctt atctgcatgc atgatcgttc ccacacgttg tgctacgatc aatacagtag
     7261 attctgtcgt ttcttttttc agacgagcac gtaacttcgc atccgtttga taatctaatg
     7321 cagaaaagct gtcatcaaaa atatagattt ccggacggcg tatgatggct ctggctattg
     7381 ccaatcgttg tttttgtcca cctgaaaaat tggaaccacc ttctgataaa tgttcattaa
     7441 atccatctgg ttttcgagaa atgaattctg aagcttgagc aatctcagct gcacgttcca
     7501 tttcttcttg cgtagcatct tctttcccgt atcgcaaatt atctgcaatc gttccagtaa
     7561 atagcagcgc tttttgcgga ataaacccaa tcttatctcg caacttgctt agccgataat
     7621 ctctcacgtc tactccatcg atcaagatct ttccttcact cacatcataa aagcgaggga
     7681 tcaattggat caacgtagat tttccgctac cggtacttcc aataaaagca acggtctctc
     7741 ctggagaggc tgtaaaactg acatttcgta tcactggctc ttgcgaatgg cctggataag
     7801 caaatgttac attttgaaat tcgatatacc ctttagtttt tgtatcagat ataccatttg
     7861 gatcttctac aatcgaaggt ttggcatcta gggcttcttg gatacgtcca gctgaaacag
     7921 ctgctcttgg atacatcatg aacacactag caaataacat gaatgagaac aaggcatgga
     7981 atatatactc gataaacgca atcaaatctc ctacacgaag ctcgcctgca tcgatttgca
     8041 ctgccccgct ccagatgatc aataccatta cgatattaaa taagaaataa aaacccggtt
     8101 gtgcaacagc catcaaacgg aataagcttt ttgaactaga agcataattc tcattgacta
     8161 cagcaaaacg tttttcttca aacttttcat tgacgaatgc acgaatcaca cgcaatcctg
     8221 aaagattttc ccgcaagata ctgttgattt tatctaagtt tttttgttgc tttttagata
     8281 acggttcaga caccttagca atcgctacga ctgccaataa tagtaaaggt aatgcaccta
     8341 ctacatacaa actgagtgtc acacttgtac gcatgaccat gtacaaactg acaatgaaca
     8401 tcattggtgc cataaaaccg atacgtaaaa tattttgcag gaacaacatg atctgataag
     8461 catcgtttgt tgtacgagtg atcaatgaag atacgcctag attttcgtat tcctgatgcg
     8521 aataactttg gatcttttcg aacaaatcat ctcgaatatc tgctacgata ttagtcgtaa
     8581 tcctcgaacc aaaataacct aacaagatat tcaaaataat accgattaca gtaataacga
     8641 tcataaatat cccttgttgc ttcacataat caaaatcatc attgcggatt cctacatcga
     8701 tcatacgtgc taaaatcgta ggaagtccta attcaatcaa aataaaacca aatacgcata
     8761 tgaaattgaa aaacaagaat ttcttataac gcatggtata atgccacatt agtttcactt
     8821 tctcatctcc ttttaatctt ttgttacatt ttcattacta aaaatgatag cacaacgagc
     8881 ctattaatag aacacttctc tcataaaaaa gtcattatga ttatagcggg atgataaaca
     8941 ttgatttttt ctttcaatct agttgatttc tatacttatt tgctgtttta tgctagaata
     9001 acaattaatt gttgtatatt ttttatacag aaggtacggt tgaaaaaata tccgaacaga
     9061 taagcaaaaa cgaggcttgt ctctttgact cgaatccagt attctagtca aagcatatga
     9121 aaaaccaggt ggcttgtccc ctcgttttgt gttgtctaaa aaaatcaact agctaaatca
     9181 ctgctttacg cagataaagg agacaaaaca atgatctata aagtttatta tcaagaaaca
     9241 aaagtacgca accctaaacg ggaagaaaca aaatctcttt acattgaggc aaaaagcgat
     9301 gtagacgctc gccaacgtgt ggaagaaaat accccataca atattgaatt cgttcaactt
     9361 ctagaaggaa gtcatctaga gtacgaaaaa gaaaatgctg atttctcatt agtggagttt
     9421 taatatgaaa ctttccatca aaaataacga gacaggcgtc tttgctattg gtggattagg
     9481 tgaaatcggg aaaaatacgt atggggtaca attccaagat gaaatcattt tgattgatgc
     9541 cgggatcaaa ttcccggaag acgatttact tggaattgac tatgtcattc ccgactacag
     9601 ctacatcata caaaacttac acaaagtaaa agccttggtg attactcacg ggcacgaaga
     9661 ccacatcggc ggaataccct acttgctgaa acaagcaaat attcctatct atgctggtcc
     9721 tctcgcatta gcgttgatca caaacaaatt agatgagcat ggcttgcttc gagatgcaga
     9781 gctccatgag atcaatgaag atacgatcat ccgttttaga aagacttctg tcagcttttt
     9841 cagaacgacc catagtatcc cagatgcatt agggatcgtg gtgaaaacac cttctggaaa
     9901 tatcgtagcg actggagact tcaaattcga tttcactcct gttggcgaac cagcaaactt
     9961 gcataaaatg gctcgtatcg gggaagaagg agtcttgtgc cttttatctg atagtacaaa
    10021 tgctgagatt ccaacattta ctaaatcaga aaaaacgatt gggcattcta ttttgaagat
    10081 cttttcaaaa atcgaaggac gtatcatttt tgccagcttt gcttctaaca tcttccgttt
    10141 gcagcaagca gcagatgccg ctgtggcaac tggtcgtaaa atcgctgttt tcggacgaag
    10201 catggaaaat gccatcgtca acggtcaacg tttaggatat atcaaagttc cagacggcac
    10261 atttgtagat gcacacgaaa tcaatcgctt gcctgccaat gaaacgatga tcctatgtac
    10321 aggttctcaa ggagaagcga tggccgcgtt gagccgcatc gcgaatggta ctcaccgtca
    10381 aatccaaatc cagccagacg atacagtgat cttttcaagt tcacctattc ctggtaatac
    10441 aacaagtgtc aatcatttga tcaatttgct gtctgaagct ggtgcagaag tgattcacgg
    10501 aaaaatcaat aacatccata cttccggtca cggtggacaa gaagaacaaa aattaatgct
    10561 tcgtttgatg aagccaaaat attttattcc tgtccacgga gaattccgca tgttgaaagt
    10621 acatgcttct ctagcacaag atgtaggtgt tcctgcagaa aactgcttca tcatggaaaa
    10681 tggcgatgtt ttagcgttaa ctagcgattc tgcgcgtcct gctgggcatt ttgtggctgg
    10741 agatgtgtat atcgatggta gcgggatcgg tgatattggt aatgtcgttt tacgtgaccg
    10801 tcacctgctt tcagaagaag gtcttgtatt agctgtcgcg acagtggata tcaagaaaaa
    10861 agaaatcttg gctggtccag atattctctc tcgtggattt gtctacatgc gagaatcagg
    10921 agaaatgatc aatgaagctc agcgaatctt attccacgct ttaagagaat gtttgaatgg
    10981 acctgactgt tcagaatata aattgaaaga agcgatgact ggcgcgcttc agcctttctt
    11041 attcgaacgt acagaaagac atccaatgat tttgccaatg gtaatgacac cagatgaaga
    11101 ataaataaga ggttatgaaa tcagcacctt gtcctgaata taagagaaaa caaatgaaaa
    11161 acatgagaag ccatttttgt ttgttcttat tccttgatac aaggtggctc tttcatagcc
    11221 tcttaaattt atttactttt tcatacaaaa aaatacctgc cacaagagaa attgtctctt
    11281 gtggcaggta ttttt
//
LOCUS       DASHHB010000077        11194 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.081, whole genome shotgun sequence.
ACCESSION   DASHHB010000077 DASHHB010000000
VERSION     DASHHB010000077.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11194)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11194)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11194
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.081"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            149..1552
                     /locus_tag="U2K71_002470"
     CDS             149..1552
                     /locus_tag="U2K71_002470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HEM7316495.1"
                     /translation="MKKSLKLASAVILMSQLLIATPFHVLASENSTYKIAKERTETTE
                     MSNSDVTAESSELLTTSSSESSTTSNAESSTTSSSESSTTSNAESSTTSSSESSTISS
                     TESSTTSSSTSEITSSKVTKPNHIESSKKEAPKTEGQTKTFSTPQQIESTESFQSQPI
                     LSGNEEIHFDKNESVENFIMKIGESARKIGQKYDLYASVMIAQAILESASGQSQLAQA
                     PNYNLFGIKGTYNGNFVIMVTNEDLGNGTLYTTQSKFRVYENYEESFEDYAKLLTKGI
                     SGNKDFYAGALKANSKTYREATKFLTGRYATDTQYYLKLNELIKTYDLTNYDKEVKGP
                     QMSKTGYQVPIHNYSITSLFGNRGNEFHRGLDMAATQGEPIHAVKAGKVVKAEFHYSW
                     GNYVVIQHEDGSTALYAHQQQYLVKTGDNVLQGQIIGYVGSTGNSTGPHLHLEICKDS
                     SLSQSMLVDPKTLLFGD"
     gene            1901..2533
                     /locus_tag="U2K71_002471"
     CDS             1901..2533
                     /locus_tag="U2K71_002471"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AP2 domain-containing protein"
                     /protein_id="HEM7316496.1"
                     /translation="MKERYGHLTVVEEITHNGYKKYRCLCDCGNETIVYRSNLLTGRT
                     KSCGCGNYKNRHKYKNLTNKTFGKLVAIKPTKLRSEGTVVWECQCLCGRKKLVSAKRL
                     LKGDVKSCGCLKSKKHHIENKKFGLLTAKYPLDNNCSWKTKWVCHCECGSMCEISYSN
                     LFFGHTQSCGCLKRQETRTCVEGTVIECLTSKLPKNNTSGVKGVYRSKEK"
     gene            3121..4560
                     /locus_tag="U2K71_002472"
     CDS             3121..4560
                     /locus_tag="U2K71_002472"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7316497.1"
                     /translation="MYSYQEDAKVQKNRWWILIAISMFTFMSTLDGSIVNIALPTISD
                     DMGIPMNQAEWVVSVYLMIVCSCLLLFGKIGDSRGKIKVFRIGTLIFVLGSLLCGFNH
                     SLGILLFARVVQAIGASMTMATNAGIITEVFPIHERGRALGAIGAFVSLGSIAGPGLG
                     GLILSQFSWAYIFWINVPVGIVTMLIAEKFLPKDITMSGHTIDGGGFAAIAATIISFF
                     GAIFLGQESGFFHPVPLVLFVISVIAFVSFLKVEKKAASPLISFGIFKNKIFTMSLIT
                     ALLIFSSNFFVNVVIPFYLQNARGLSPSYAGLLMMVFPLMMVVGAPLSGYLTDRVGPE
                     ILVLVGLGLLSVTQVMYMFLSVDSPLWLYIVATAIMGAGNALFQSPNNTMVMSSVTIE
                     NLGLAGSMNSFARNLGMVSGISLSTTVLYQAMSAKMGQQVTNYIANRPDVFLYGMKIT
                     FLGSFVLCFVAFFLTIWRIKKQGGIQKNV"
     gene            4855..5073
                     /locus_tag="U2K71_002473"
                     /pseudo
     CDS             4855..5073
                     /locus_tag="U2K71_002473"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016251366.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            5322..5537
                     /locus_tag="U2K71_002474"
     CDS             5322..5537
                     /locus_tag="U2K71_002474"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316498.1"
                     /translation="MNAGIADMNLIKKTLNDFTSNSISKGTGINLSTIKKLKSGERSV
                     EKLNLLDAIKITEFAMKNGKAEIEIWR"
     gene            complement(5754..6010)
                     /locus_tag="U2K71_002475"
                     /pseudo
     CDS             complement(5754..6010)
                     /locus_tag="U2K71_002475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(6197..7108)
                     /locus_tag="U2K71_002476"
     CDS             complement(6197..7108)
                     /locus_tag="U2K71_002476"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM7316499.1"
                     /translation="MKIKIERNQKEKSPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GVYKNFTAIDQHTTHEKVVIKEKVVNTSGVESFTEDFAKEYFSWKNNKEAIEKRMSNL
                     EQYLTENGLALIQDMVRADIPTSSEVQSVKILDVEKHSEEFVVSFLVEQHITEGKKTQ
                     SISSAYRVTIFEDENRNHLVTSLPTMIGKPDKAKYEAKQVENDSEIDAKTTEEITDFL
                     ETFFKLYPSASEKELEYYVENGAMRPINIDLKFSELENPVYLQNGKNFLVSTKIKYLD
                     NVSKTTSYFQYKLVLQKNGNWKIVDVK"
     gene            complement(7127..8131)
                     /locus_tag="U2K71_002477"
     CDS             complement(7127..8131)
                     /locus_tag="U2K71_002477"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEM7316500.1"
                     /translation="MNLKKVGVISLTFLTISFMGILMCVGLLFGEDSEGNGSSGTSPG
                     GTSVSEEVLKHRAMVEKYAKESDITEYIPILLAIIQVESGGTMEDVMQSSESAGLPPN
                     SLSTEASIKQGCLYFASLVKRSKELGCDQDSIIQAYNYGGGYLDHVGKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENGGWRYNYGNMFYCLLVKQYLTTTQFDDKIVQEIF
                     DEAFKYEGTAYVFGGSSPETGFDCSGLTQWCYAKVDLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLVFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTENYWRQHLIGAGRYK
                     "
     gene            complement(8128..10251)
                     /locus_tag="U2K71_002478"
     CDS             complement(8128..10251)
                     /locus_tag="U2K71_002478"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002407825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM7316501.1"
                     /translation="MKKKLFRIVGIVFISILTLIVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAESIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRAVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALTLGTEIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEDRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKKAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRALAFGGGAAVGNKLAKEQSKSKFKPSGSSLRKNSRLPNDHEILSDLTKEN
                     PVSNNKKQSRMNLAGRKIGKVLDTQALVKDKTKQVKDQVRNTPTNLKYNLHKGIEKSK
                     KAPEEFKRGLFQEKADRVDLREKQRQRRNAKMDEKRKILSEATNRQGKRRANIPIDKA
                     AQPQKDMMLKKEAPKRIVRANLNPEIKRKLTSQEIISKGKSQSASKKNSFQQVKQRST
                     LPKQTNQKVQKRMNHRPKSRSGEKK"
     gene            complement(10256..11176)
                     /locus_tag="U2K71_002479"
     CDS             complement(10256..11176)
                     /locus_tag="U2K71_002479"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM7316502.1"
                     /translation="MPEIADEINILNLTSKPKNQGLLDPYVIMEKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSNQRGLLQVINELRKEGSPVAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDRQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDREDESNQKRLRDLENGQCLMQDLY
                     GRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         3471 a         2104 c         2083 g         3536 t
ORIGIN      
        1 tttaaaggca gtaacaggat taaacattga aacataatcg ttactaaatg aaatcaagaa
       61 atatactgta ttactcaatt cattgaaaat atttatcata atttttaata gaaaacacta
      121 ttaaagagaa aaaagagagg taaatcaaat gaaaaaatct ttaaaacttg catcagcagt
      181 tatattaatg tcccaactcc ttatagcaac acctttccat gtgttggcaa gcgaaaattc
      241 aacatacaaa attgcaaaag aaagaaccga gacgactgaa atgagtaatt ccgatgtaac
      301 agcagagagt agtgaacttt tgacaacttc atcaagtgaa agtagtacga cttcaaatgc
      361 cgagtcttca acaacttcat caagtgaaag tagtacgact tcaaatgccg agtcttcaac
      421 aacttcgtca agtgaaagta gtacgatttc aagtaccgaa tcttcaacaa cttcaagtag
      481 tacaagtgaa ataacttctt cgaaagtgac aaaaccaaac catatagaat cttcaaaaaa
      541 ggaagcgcca aaaacggaag gacaaacaaa aactttttcg acaccacagc aaatagaatc
      601 aacagaatct ttccaatcac aaccaatact atctggtaat gaagaaattc attttgacaa
      661 aaatgaatct gtagaaaatt ttattatgaa aattggtgag tctgcgagaa aaattggtca
      721 gaaatatgat ttatatgctt ctgttatgat tgcacaagcg atcttagaat ctgccagtgg
      781 tcaaagccag ttagctcagg caccgaatta caatttattt gggataaaag gaacttataa
      841 tggaaacttt gtcattatgg taacaaacga agatttgggt aatggaacgc tttatacgac
      901 ccaatcaaaa tttagagtgt atgaaaacta tgaggaaagc tttgaagatt atgccaaatt
      961 attaactaag ggaattagtg gaaataaaga tttttatgca ggagcattaa aagctaatag
     1021 taaaacatat cgagaagcaa ctaaattcct tacaggacgg tatgcaacag atacacaata
     1081 ctatttgaag ctaaatgaat taattaaaac ctacgattta accaactatg ataaagaagt
     1141 taaaggtcca caaatgagta agactggata ccaagtgcct attcataatt attcaataac
     1201 cagtttgttt ggaaatcgtg gaaatgaatt tcaccgagga cttgatatgg cagctacaca
     1261 gggtgagccg attcatgcag ttaaagctgg taaggttgtt aaagcagagt ttcactactc
     1321 ttggggaaat tacgtggtga tacaacacga agatggatca accgcgttat atgctcacca
     1381 gcaacaatac ctagtaaaaa ctggagataa tgttttgcaa ggacaaataa ttggctatgt
     1441 aggaagtact gggaatagta ctggcccaca ccttcatttg gaaatttgca aagatagtag
     1501 tctttctcag agcatgttag ttgatccgaa aaccttatta ttcggagact aaaaaaggta
     1561 atcaatctga caaagtaaca agaaaatggt tggattctta gacctttgat caggatattg
     1621 agactggaat tgatatctcg attacgatgg gtttgacaaa taaaacaagt ccattatcga
     1681 atatcgagtg tattattgcc gtctttatgc tcatattttg agcaggtttc actagatgaa
     1741 aacaatttat ctatcttgag gatttcgtgt acgtatcgtt cagtcttgta ttgtaaatta
     1801 gtcataaaat cagactaaga aaaattggaa atattttttt ctgatttatt aaaagttaat
     1861 tgaaattata tggtacttct ttcttgggag ggtaggtgca gtgaaggaaa gatatggtca
     1921 ccttactgtt gttgaggaga ttactcataa tggatataaa aaatatcgtt gcctatgtga
     1981 ctgtgggaat gaaacgattg tatatcgatc aaacttacta acaggtagaa ccaaaagttg
     2041 tggatgtgga aattataaaa ataggcataa atataaaaat ttgacaaata aaacttttgg
     2101 taagttagtt gcaatcaagc caactaaact gcgaagtgaa ggaactgttg tttgggaatg
     2161 tcaatgtctg tgtgggagaa aaaagttagt atcggcaaag cgacttctaa aaggtgatgt
     2221 aaaaagctgt ggatgtttga aatcaaaaaa gcatcacatt gaaaataaaa aattcggatt
     2281 acttactgca aagtatcctt tggacaataa ctgttcatgg aaaacaaaat gggtttgtca
     2341 ttgtgagtgt ggcagtatgt gtgagataag ttatagtaac ttattttttg gacatactca
     2401 aagttgtgga tgtttaaaaa ggcaagaaac aaggacatgt gttgaaggga cagtaataga
     2461 gtgtctaacg tcaaaattac cgaaaaataa tacaagtggt gttaagggag tttatcgttc
     2521 taaagagaaa tagattgcgt atataacttt aaaaaaaaag aggtactatt taggatcgta
     2581 tgattctatt aagagtgcag ctcaagctag aaaagatgct gaaaataaat tgtttcaacc
     2641 tttgattgac aaatatcgag gtaatcatta attattgtgg ataataatta acagtgatat
     2701 ataaacatct ataatatatt attcatacta ttgatattta gtataatcct atggaattga
     2761 atatcatagc aatgtatttg aaaagcaaat tggaaaacaa ataggtgcaa caccataaat
     2821 atgaaaaaat tgggaaaaag tagaatggaa atttgcttac gtctcacgag atagctcaca
     2881 ctattaatct gaagaacaat gaagtcgact tttagaaatt gctagaagaa agtaaagcga
     2941 attatcacta gttactatag agtaagttca aataaagcat ctccaactta catggttaca
     3001 tgaggatatc aaatcgaaat tactcaagct tcctatggct agagtcgcga gaagttgttg
     3061 ggcagtagac ataggttata ttgttcttag ctacacgatc attagaaagg aaatcctatt
     3121 atgtatagtt atcaagaaga tgcaaaagtc caaaaaaatc gctggtggat tttgattgct
     3181 atatctatgt ttaccttcat gtccacattg gatggcagta ttgtcaatat tgcactacct
     3241 accatctcag atgatatggg cataccaatg aatcaagcgg aatgggtagt gtccgtttat
     3301 ctgatgattg tctgttcttg tttgttgctg tttggtaaaa ttggcgatag ccgtgggaaa
     3361 atcaaagtat ttcggattgg tacgctgatc tttgtcttgg gatcgctgtt gtgtgggttc
     3421 aatcattcgc taggtatttt attgttcgca cgggtggtac aagccattgg tgctagtatg
     3481 actatggcta caaatgccgg aatcatcacg gaagtctttc ccatacatga acgtggtcgg
     3541 gcattgggag caataggtgc gtttgtttca ttaggctcga ttgctggacc aggtcttggc
     3601 ggcttgattt tgtcccaatt ttcttgggcg tacatttttt ggatcaatgt accggtggga
     3661 atcgtaacga tgttgattgc tgaaaaattc ttaccaaaag atatcaccat gagcggccat
     3721 acaatcgatg gagggggctt tgctgccatt gcggctacga ttatcagctt ttttggagca
     3781 atctttttgg ggcaagaaag tggctttttc catccagtcc cccttgtact gttcgtaatt
     3841 tcagtgattg cctttgtcag ctttttaaaa gttgaaaaga aagcagcctc tccattgatt
     3901 tctttcggta tttttaagaa caagattttt accatgagct tgattacagc cttgttgatt
     3961 ttttcttcca acttctttgt caatgtagtg attccttttt atctgcaaaa tgctcgcggg
     4021 ttgagtccta gctatgctgg gctgttgatg atggtctttc ctttgatgat ggtcgtcggg
     4081 gcgcctttaa gcggctacct gaccgaccgg gttggcccgg agattttggt attagtaggc
     4141 ttgggcttat tgtcggtaac ccaggtcatg tatatgtttt tgtcagtgga ctctcctttg
     4201 tggctataca ttgtcgctac tgccatcatg ggtgccggca atgccttgtt ccagtccccc
     4261 aataatacga tggttatgag ctcggtcact atcgaaaact tgggactggc aggctcgatg
     4321 aattcctttg cccgaaattt ggggatggtc agcgggattt cgctttctac gacggtctta
     4381 tatcaagcga tgtctgctaa gatgggccag caagtaacta actatatcgc aaatcggccg
     4441 gatgtctttt tatatggaat gaagattacc tttttaggca gctttgtttt gtgttttgtt
     4501 gcctttttcc tgactatctg gcgtatcaaa aaacaaggag gtattcagaa aaatgtctga
     4561 aaaaaccact gttgaagaat ttttggaggc ctgcgtatgg gtgagtggga tttccatgga
     4621 tgatggaaga tgaagtgaac ttctaaaagc cgaaaaactc aagtattatc ttcaaaatac
     4681 tagtgctcaa tgagatgctt gatttgatca caagaagact gcgaaagccg ctggcaaccg
     4741 caagctaggt atcccaccga tggactacgt tttagaggct actggttatg aatacggagc
     4801 caacgctctc atctgcataa gaatacatca cccagattat gtaaagattt tcgatacttc
     4861 tgtcaaaaaa tcattaagat agataaatga tttccgtcta gtcaaattgg ttcagaatgt
     4921 agacacacag atggtaagaa gtcactcaca attcgagaat ggacttgtcc tatttgccgt
     4981 atacatcata atcgaaacgt caacgatagt atcaatattt tgaccaaggg ctatgaatac
     5041 aagcattgcc ttagaaaaca tcaagaacct taggaacaac ggggatagct tggttaataa
     5101 gagataccgc tgtttaggct tgcaagtata ctcttttatc aagaattccg tggctttagt
     5161 catgagaggc ttaatactat gaaaattttc tttagcattt ttgatttagg ttccttttct
     5221 aattataata agatgaccac tacagtaacc tactgaaata tataaacagc cactgtagta
     5281 ccattttcaa agtagtctat gagaggagaa tcttcattct tatgaacgct ggaattgcgg
     5341 atatgaattt aataaaaaaa acccttaatg attttacttc caattcaata tcaaaaggta
     5401 ctggtatcaa tttaagcaca ataaaaaagc tgaaaagtgg tgaaagatca gtggaaaagc
     5461 tgaatctttt agatgctata aaaataacgg aatttgctat gaaaaatgga aaagctgaga
     5521 ttgaaatatg gagataatta tcgtctagtg aattacttgc cacttagctg aatttaacga
     5581 tccttggtgc ttgaaggggg agattcttgg gaatagagca tacttgcaag cctatgtctt
     5641 tactaacaac gatatctctt attaaccaag ctatccccgt agttctaagg cttttacttt
     5701 tatctaagca ataatcgtat tcaaaggaaa tttttgaccc ttagaaagtc tcattttatc
     5761 agacgtgagc ttaggcattg ctgaattttg ttaattcggt tttaggttgt gctttgagca
     5821 taatatgaaa gtattcttta tcacgatttc attcatctaa ggtaatgtga tgtgattctg
     5881 aaattctttc aaaggcagtt tttgcataag ctgacattac atcatcaatg acatgtttac
     5941 aatgtttcac tactaaaata agggggtaac caagaagaaa aactgaatga ttatttgtat
     6001 ctaattccat ggtatatcaa ccatttccta tatagactga ttatgacatg gattaaaata
     6061 aaaaggcact atgtcttaat tgtcggtttt cgagttaccg aatggcattc atcacccacc
     6121 tatagaggat gggcgacttc tgcctgaaat aagttacact acttataact tctaataaaa
     6181 ttagattgag tcattattat ttaacatcaa cgattttcca gtttccattt ttttgtaata
     6241 ctaatttgta ctgaaagtag ctagtggttt ttgatacatt atcaaggtat tttatctttg
     6301 tactcactag aaaatttttt ccgttttgta gataaactgg attctctaac tccgaaaact
     6361 ttaagtcaat attgattggt cgcatcgcac cattttcaac ataatactcc aattcttttt
     6421 cagaagctga tggataaagt ttaaaaaatg tttctaaaaa atcggtgatt tcttccgttg
     6481 ttttggcatc aatttctgaa tcgttttcta cttgttttgc ttcatatttt gccttgtctg
     6541 gctttccaat cattgttggc aaactagtta ctaaatgatt cctgttttca tcttcaaaga
     6601 ttgtcactcg atatgcagaa gaaattgatt gcgtcttttt tccctctgta atatgttgct
     6661 ccactaaaaa tgaaacgaca aattcttccg agtgtttttc aacatctaga atttttacag
     6721 attgaacttc ggaactcgtg ggaatatctg ctcgaaccat atcttggatt aaagcgagac
     6781 cgttttctgt aaggtactgt tcgagattac tcattctctt ttcaattgct tctttgttat
     6841 ttttccatga aaaatattct ttcgcaaaat cctctgtaaa actttcaacg ccagaagtat
     6901 tcactacctt ctcttttata accacttttt catgagttgt atgttgatca atggcggtga
     6961 aatttttata aacgccaaaa gcaagactac aagacaataa aagccaaagg gcaagcacca
     7021 tttttcgatg cttacccacg gatacaactc gttctttttt cttttttggg ctcttttcct
     7081 tttgattccg ttcaattttt atcttcattc tttttcctct tttctcttat ttgtatcggc
     7141 cagctccgat aagatgctgc cgccaatagt tttccgttag attcgtccac cccactggat
     7201 ctcctgcatg aaacatgcga ttctcccctg cgtaaatgcc cacatgggtc acataagtac
     7261 cggcatcata ggttgaatga aagaaaacta aatcacctgg tttggcatct tttagatcaa
     7321 tatgagtcat ggcatcatat tgatcctgag ccacccgagg aagtttcagg tcaactttgg
     7381 cataacacca ttgggtcaag ccactacaat caaaccccgt ttctggactt gagccaccaa
     7441 acacataggc cgttccttca tacttaaatg cctcatcaaa aatttcttgg actattttgt
     7501 catcaaactg agttgtagtt aggtattgct tcactaataa acagtaaaac atattgccat
     7561 aattataccg ccacccacca ttttccttaa tcgcgattgg gttggggtaa tcgacttttt
     7621 gaccacctga tttttccttt gaaaaagatt cagctaaagc aaaactatgc ttcttcccgt
     7681 ttttcccgac atgatctaga tacccgccac catagttata ggcctgaata atactatctt
     7741 gatcacaccc tagttctttg gaacgcttaa ccaaagacgc aaaatagaga cagccttgtt
     7801 tgatggatgc ctcggtactt aatgaattag gtggtaatcc ggctgactca gaactttgca
     7861 taacgtcttc cattgtgcca ccagattcca cttgaataat cgccaaaaga attggaatat
     7921 actctgttat atccgattct ttggcatact tttccaccat tgcacggtgt ttcaatactt
     7981 cttctgagac actagttccg ccaggagaag tgccagaact accatttccc tcactgtctt
     8041 caccaaacaa caaacccaca cacatcaaaa tacccataaa agagatggtc agaaaagtta
     8101 atgaaataac acccactttc ttcaagttca ttttttctca cctgaccttg attttggacg
     8161 atggttcatt cttttttgta ctttttgatt ggtctgcttt ggcagagtgg acctctgttt
     8221 cacttgttgg aaagaatttt tcttacttgc tgattggctt ttcccttttg aaatgatttc
     8281 ttgactagtt agttttcgtt tgatttcggg attcagattt gctctcacaa tccgctttgg
     8341 tgcttctttt ttcagcatca tatctttctg aggttgagct gctttatcta ttggaatatt
     8401 cgcccttctt tttccctgac gatttgttgc ttcactcagt atcttgcgtt tttcgtccat
     8461 tttcgcattt cttcgttgtc gttgtttttc tcgcaaatcc actcgatccg ctttttcttg
     8521 gaacagtccg cgcttgaatt cttctggtgc ttttttcgac ttttcgattc ctttatggag
     8581 attgtatttc aaattcgttg gcgtattcct tacctgatcc tttacttgtt tagtcttatc
     8641 tttcaccaaa gcttgggtat ccaaaacttt gccgattttt ctcccagcca aattcattcg
     8701 ggattgtttc ttattatttg aaactggatt ctccttagtt aagtcagata gtatttcgtg
     8761 atcgttcggt aatcgtgaat tctttcgtag agaagaaccg gatggcttaa atttggattt
     8821 tgattgttcc tttgccaact tatttccgac tgctgcacca ccaccaaaag ccaaagctcg
     8881 tcccacattt cgattgagtt gccgcaattt tcgattcatt aacatttgcg gttttcgcat
     8941 gacgcgtctt cccattgact ggctatcatt actctggagg ttgaacatac tcataatgtc
     9001 gcccaatttg aaatagatac cggcaaaggt cacaatttgc agaaaagcaa ccatgaaaaa
     9061 tggataggtt gccgaaatat tgaaaaacat cgtggagatg ctaaatgcag tcgtaatcac
     9121 caaagtaaca cccgctcgca tcataatcgt attgaataaa cgaataatcg ccttctttcc
     9181 taaactttca taagtcggca acatggacaa taagaaacta attggcagaa acatcgcaaa
     9241 gataatgaag agaatttgcg agaacaacat aatccctgtt agaagaaaaa caaagaagga
     9301 aatgccaata ttgaacaata ccaaaaagac cacggtccct aaacggttca tggtcttggt
     9361 aatactgaga ttcgcattgt cattgtcttc aatctctgcc ttgaccgctt cttcccgatc
     9421 tttgcctttg ttttcatctg gactcacaga tagaatcttg ttgacccggt cttcaccaat
     9481 ttcttctatg ttggaatcat caaattgcag tagtaaccaa ggttgttgga cttgaatcga
     9541 aaacaaacta tcccgaatca aatccacact atcttttcct tggctttctg aattgggtac
     9601 aacgatctcg gttccaaggg taagggccgc ttcactaaca tctgagctaa agtcattgat
     9661 ttttgtgata taggtaggcg cataagcaat gaacgaaccc gatagtagaa aaatcatcac
     9721 aaaattaatc acggctcgaa ctgctttcgt ggtttctcgt tttaataatc cggtatacgc
     9781 cacatacacc cccagagcta aaatcatcaa taatagaaac ccaaaataaa atccggaggt
     9841 ctggagtcca ttttcagtaa taccagccaa tgtttggata ttctttccaa tactttcagc
     9901 cgtatccgaa atgaaatcta atttgtaagc ctcttgaacc acataacccg tggcgttcga
     9961 caaatacaaa ctcaccgtcc aaatgaaatt agtaatggca taaagaccat acatcacgct
    10021 tttccctaag ccatcacccc aattccaagg caaccaatcc caagagctat ccacaaagaa
    10081 atctagttga tagttgttca gtgaatattt ggaatataga tttcctgctt caactgtatc
    10141 atcaactagc cctgtcgcct cggctaccgt accaactaaa ctcaatagaa ctatcagagt
    10201 gagtatgcta ataaaaacga ttcccacaat ccgaaaaagt ttctttttca tcccctcact
    10261 ccattctctc ttgaacaggt ggacgggtat caaaggcatg gaagagttct tcaaagactg
    10321 gatgtacctg aaccacgcct acccgaccat acaaatcctg catcaagcat tggccatttt
    10381 ccaaatctcg taggcgtttt tgattgcttt catcttctcg atccaagcca aagaattcca
    10441 aggttttctt gatttcatga atatcggtac tgcgaaacgc aaacttcaac ccgatattat
    10501 tcttcaagcg ttcatccagt aaatcatcac tattttgcgt gacaaagtaa accccggcat
    10561 tcatagcccg cccagcacga acgagcttat tcgataatgt tttcccttgc gctacttgaa
    10621 gaaaactcca cgcctcatct aagtctacaa ttttgaagat ttctcgatta ctatggataa
    10681 agtccaaggc aaaagtggaa atgacgatta gcatggaaac actaagcaat tccatggtgg
    10741 tatattcttc aaaagtggtt tctgcatctg gtaaaaccaa atccgctact tgaatgatat
    10801 ttaactgcct atctaaacta atcgactggg taatcgaacc atctgagaaa agcaaatggg
    10861 caaaatcata atcggtaaag gactcgatgt gatccgcgat attttccgca actggtgagc
    10921 cttcttttcg aagctcatta attacttgta ataatccccg ttgattgctt tgggtaaccg
    10981 tccgaatggc cttacgcaac actggaaact tctccccatc tcgactagaa attcctgtta
    11041 aaaaggtcag aatatcaatt gctagacttt cagaatcctt ctttttctcc ataatcacat
    11101 aaggatcaag taatccttga ttttttggct tactagttaa gttgagaata ttgatttcat
    11161 ccgcaatttc tggcaaggtt tctttccagc cacc
//
LOCUS       DASHHB010000078        10410 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.082, whole genome shotgun sequence.
ACCESSION   DASHHB010000078 DASHHB010000000
VERSION     DASHHB010000078.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10410)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10410)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10410
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.082"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(46..1365)
                     /locus_tag="U2K71_002480"
     CDS             complement(46..1365)
                     /locus_tag="U2K71_002480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008392738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM7316503.1"
                     /translation="MKKLKIFPKMFIQIFSVLGIIIILVHSLIFFIFPKTYLETRKEK
                     IHNMANEISSNMNGKEIKYIEQTLEFYSKSSEIKVFIKEKNNKNEIQIKDNINVNLES
                     DSNSLIIEEREIKLNDGKKTNLQFVSTADMQKDAKDLSLKFLPYSLLISILFSAIISL
                     IYAKSIKNNIQEIKIVTDKMMKLDKKMSLKVSSNDEVGELKQQINDLYSTLLRTIDDL
                     EFKNKEILKLEKLKYDFFKGASHELKTPLASLKIILENMKYNIGKYKERDIYINECIE
                     IVDSLTKNISQILSVHSIENLNNDEEYLKINDTLEDILKKYEILANQKKITVNNYILD
                     ENVYIGKTALKIILSNLISNAVKYTDVNGVINIGIVNDWLYIENSYGNNKISNMDKIF
                     DVKFDLNKENSNGLGLYIVSNILNNYNIEYKVLQDEEFFIFKIKIFS"
     gene            complement(1362..2015)
                     /locus_tag="U2K71_002481"
     CDS             complement(1362..2015)
                     /locus_tag="U2K71_002481"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7316504.1"
                     /translation="MKILTVEDDNMIREGISEYLSEFGYTVIQAKDGREALSKFNSDI
                     NLVILDIQIPFINGLEVLKEIRKKSNLPILILTAFSDEEYKIDAFTNLVDGYVEKPFS
                     LPVLKARIDSLIKKNYGHLEKFEYKDLSVNFNSYTAKINGEEIDVNAKELEVLKCLLD
                     NNGQVLTRMQIIDYVWKDSEETPYDRVIDVYIKELRKKLQLDCITTIRNVGYKLERK"
     gene            2303..2823
                     /locus_tag="U2K71_002482"
                     /pseudo
     CDS             2303..2823
                     /locus_tag="U2K71_002482"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340466.1"
                     /note="frameshifted; incomplete; partial in the middle of
                     a contig; missing C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
     gene            complement(2725..3441)
                     /locus_tag="U2K71_002483"
     CDS             complement(2725..3441)
                     /locus_tag="U2K71_002483"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HEM7316505.1"
                     /translation="MIKEKIDSFVTIDDLQVGKPDPEGYLLCLKRLRVEQAVVVEDSP
                     VGAQAAPHMTVDVQALDADFFAFSGHKMCGPTGIGVLYGKRQLLEQMEPVEFGGEMID
                     FVNLYDSSWKELPWKFEAGTPNIAGAIDYLNEIGMENIHRYEQELVAYVLPKLQAIGG
                     ITIYGPQDPEHHTGVVAFNLDGIHPRDVATALDMEGVAVRSGHHCAQRFIDDFCLTNW
                     SFKQSMIMPFERLQCRGIFE"
     gene            complement(3491..3700)
                     /locus_tag="U2K71_002484"
     CDS             complement(3491..3700)
                     /locus_tag="U2K71_002484"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316506.1"
                     /translation="MDVLREYSSLFTEAYLLEQFGYKIPFADYQQGVGTAAELSFYEG
                     ISQAVGRPVDAEHFEQRISSTGKSE"
     gene            3977..5250
                     /locus_tag="U2K71_002485"
                     /pseudo
     CDS             3977..5250
                     /locus_tag="U2K71_002485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="frameshifted; internal stop; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
     gene            complement(5357..6388)
                     /locus_tag="U2K71_002486"
     CDS             complement(5357..6388)
                     /locus_tag="U2K71_002486"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7316507.1"
                     /translation="MFLEIHDLNLFFDEKQILAIDEIQIEKGELVTLLGPSGCGKSTL
                     LRCITGLEQPKSGEIVLDNENIADKPTKDRNIGFVFQQYALFPTMTVFENVAFGLKVK
                     KLSPEVIQEKVFEMLSLVDMTEQADKNVQFLSGGQRQRVALARSLVTEPKVLLLDEPL
                     SALDARIRKQLQRDLRAIQQSLGMTMIFVTHDQEEAMRISDRIFVMEAGRVAQVSTSK
                     ELYQNPQSRFVAEFIGNYNRFEWYELNQYTHEFSAKGNRCIYYLRPELIQFEPVPQGV
                     KIPVLWKESFILGNIQRYVFQTLEGKEILVDRLNDREEKISDALYIQLKDILAIPVEI
                     DTQHFAQQS"
     gene            complement(6395..7225)
                     /locus_tag="U2K71_002487"
     CDS             complement(6395..7225)
                     /locus_tag="U2K71_002487"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7316508.1"
                     /translation="MKKLIKPILFCFVLYYLLPIIGTILYASSTKWSKSLLPSDFTLQ
                     WFQQLLTDREFIAAVGRSLLLAGVVLVTILLLMIPTIIWIHLYFPRLNRWLEKLLLLP
                     YALPGVILVTALLRTYAETGIPMFVVLVGALFITALPIVYLSLNNQMRLINLKELVDA
                     AETLGAPMSTIIIQVLFPNIRIGVTLVSLMIFSSVFGEYMLTNLLIGGRFQTVRIYML
                     RRMNENGHLASAVMVLYLLFMVGIALTTFMLTYRQKQQLSLKQTREEKQSKKKPLKEL
                     "
     gene            complement(7222..8037)
                     /locus_tag="U2K71_002488"
     CDS             complement(7222..8037)
                     /locus_tag="U2K71_002488"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM7316509.1"
                     /translation="MKEMKKRLVLVPASFIFCAFLLLPLLFMVLCSLHSDLTNQWTLA
                     NYSDIFSNKYYTQAFVNSVLIAVSSSVIGLIGTFILCLCLMNLSTKLQEKITFVSNLA
                     ANFAGIPLAFSFIILLGNVGVLKMILPFLADFNLYSWWGLTITYTYFQIPLGVLFLYP
                     SMKEVKKEWLESAQLLGASSAYAWFKVALPFLRPSIASTFVILFANGMGTYETAYALT
                     SSNVNLLTIRIAALVSGDVFAKPNLGSALAVALGVLLVTAMLIIQKKGKVVQT"
     gene            complement(8034..8858)
                     /locus_tag="U2K71_002489"
     CDS             complement(8034..8858)
                     /locus_tag="U2K71_002489"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HEM7316510.1"
                     /translation="MQSRLDKLIVVILDGCRYDTAVAQLGFMNHLVEHQQASLLKVKS
                     ELPSNSRPLYEVLMTGVPAYENGIYSNYHAQRSKEWSLFELVKEAGGKTGAAAYHWYS
                     ELYNQAPYNRLVDRIQHQEERLIQHGIFYSEDTYPDSHLFADAHYLVNQYQPDFLVVH
                     SMNIDDVGHKFTADSHEYAAAVNQADRLLAIYLPEWLQKGYQVVVTADHGMDAHGLHG
                     GSLAAHREVPFYLISNKRQKLKEQTVPQLLAAPLFCWLLGIAPSENMQDIQELMRG"
     gene            complement(8848..9948)
                     /locus_tag="U2K71_002490"
     CDS             complement(8848..9948)
                     /locus_tag="U2K71_002490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HEM7316511.1"
                     /translation="MKMNEKIRNMTIGLFCAATVSILLTGCGSGEATKQSAAADDSLD
                     TIIEKAKEEGEIASLGMPDTWANWVGTWNDLEKEYGLSHVDTDMSSAEELAKFESEGK
                     NGTADIGDVGISFGPLAESKELTLPYKTNYWEEIPDWAKDDDGDWLLSYTGTISFITD
                     KENVKQAPTSWKELQEGDYQVSVGDVTIANQAQFAVLAAAIANGGDEKDIQPGLNYYK
                     KMAEKGRLSTVDASVANLEKGEIDVAVVWDFNGLNYRDQIDSERFEVTVPTDGSIISG
                     YTTLINKNAPHPNAAKLAREYILSDEGQINLAKGYARPIRKVELPKEVAEKLLPEDAY
                     EAAVPVEDNEAWNKTSVELPELWQAEVLTYAK"
     gene            10126..>10410
                     /locus_tag="U2K71_002491"
     CDS             10126..>10410
                     /locus_tag="U2K71_002491"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEM7316512.1"
                     /translation="MQYYDQHLHTYFSFDSEEKFENYLAYQPEFFVSTDHFDLNNPST
                     GKDDIPDYPAYINKLDSLKETYSTKFLRGIEIGVVPGQEERIVDYLAQHPY"
BASE COUNT         3195 a         2298 c         1695 g         3222 t
ORIGIN      
        1 aataagtctc tatttttcaa taactattgc aaaattatat cttaattaag aaaatatttt
       61 tattttaaag ataaaaaatt cttcatcctg caatacttta tattctatat tgtaattatt
      121 cagaatatta cttacaatat ataaacctaa tccattacta ttttccttat ttaaatcaaa
      181 tttaacatca aatattttat ccatattcga aattttattg ttaccgtatg aattctctat
      241 atataaccaa tcattaacta tcccaatatt aattacccca tttacatcag tatattttac
      301 cgcattacta atcaaattag atagaataat ctttaaagct gtttttccta tataaacatt
      361 ctcgtctaat atataattat tgacagttat tttcttttga tttgctaata tttcatactt
      421 ttttaatatg tcttctaatg tatcatttat tttcaaatat tcttcatcat tattcaaatt
      481 ttctatagaa tgaactgata atatttgaga aatatttttt gttaaactat caacgatttc
      541 aatacattca ttaatataaa tatctctttc tttatatttt ccaatattgt atttcatgtt
      601 ttccagtatt attttaagac tagcaagagg ggttttaagt tcatgggatg cacctttaaa
      661 aaagtcatat tttaactttt ctaattttaa aatttcttta tttttaaatt ccaaatcgtc
      721 aattgttctt aataaagtag aatacaaatc attaatttgt tgttttaatt ctccaacttc
      781 atcattagag ctaactttta aactcatttt tttatcaagt ttcatcattt tatcagtaac
      841 aatttttatt tcttgtatat tattttttat tgattttgca taaattaaag aaataatagc
      901 agaaaataaa attgatatta ataaagaata tggtaaaaat ttaaggctta aatctttcgc
      961 atctttttgc atatcagctg tagaaacaaa ttgtagattt gtttttttac catcattaag
     1021 ctttatttct ctttcttcaa ttatcaaaga attactatca ctttctaaat taacatttat
     1081 attatctttg atttgtattt cattcttatt atttttttct tttatgaata cttttatttc
     1141 actactcttg gaataaaact ccaaagtttg ttctatatat tttatttctt ttccattcat
     1201 attactagaa atttcatttg ccatattatg aatcttttct tttctcgtct ccaaatatgt
     1261 tttaggaaag ataaaaaaaa ttaatgaatg aactaatata attattattc caagaacaga
     1321 aaatatttgt atgaacattt ttggaaatat ctttaatttt ttcattttct ctccaattta
     1381 tatcctacat ttcttatagt agttatacag tctaattgta atttttttct aagttctttt
     1441 atatatacat ctattactcg atcataagga gtttcttcgc tatctttcca tacataatca
     1501 ataatttgca ttcttgttaa cacttgtcca ttattatcca ataaacattt taatacttct
     1561 agttcttttg catttacatc tatttcttca ccatttattt tagctgtata gctattaaaa
     1621 ttaaccgata gatccttata ttcaaacttc tctaaatgtc cataattctt cttaattaaa
     1681 gaatctattc tagcttttaa gaccggcaat gaaaatggct tttctacata tccgtctact
     1741 aaattagtaa atgcatcaat tttatattct tcatcactaa atgcagtcaa gattagaatt
     1801 ggtaaattgc ttttctttct aatttctttc aacacttcta aaccatttat aaaaggtatc
     1861 tgaatgtcta agataaccaa atttatatcg ctattaaatt ttgacaaggc ttctcttcca
     1921 tctttagctt gaataacagt atatccaaat tctgaaagat attcacttat tccctctctt
     1981 atcatattat catcttcaac agttaatatt ttcatatata cctccactta agcctataca
     2041 ctagatatta tacagcatat tatggctcta taatatctag tgttggtgac gccttctgtt
     2101 gccaatactt tttctttttt ttaataaatg caaacagaca ccttgaaatc ctttagaaaa
     2161 aaggcttcta taatattttg tgttgttgga aaatcgaagg cgattttcta ctaacacttt
     2221 ttcttcgttt caactcaaaa agagactcac ttttggtaaa atttagtcga ccaaaacatc
     2281 accctaccaa aggagtcgct ctatggataa tcatactaga aaattactcg gattaacaga
     2341 taaacattta ttttttgatg aagaatggct gattgaaaaa gaatataaag gcgttcaagc
     2401 gcagtttatt aaagggagat tggatgactc accttcccat tgtgaacact gtggatcgat
     2461 tcgtattatc cgcaacggtt cctatacgac acgtactcag atgttgaaag tcaaagaaaa
     2521 gttgactatt ttagaattaa aacgtacccg ttttctatgc tatgattgtg ggcagacctt
     2581 ttctgcgaaa acggacttag ttgatgaaca tcatcaacta acaaaggaac tgaaacaggc
     2641 gattctcata gaactgtacg aaaatcaatc acgtaatctg aaaatcagaa gaagtaccgt
     2701 cggctcaagc gatattggaa actcttactc aaagattcca cgacattgga gccgctcaaa
     2761 cggcattatc atcgactgtt taaacgacca attagtcaaa cagaaatcgt cgatgaaccg
     2821 ttgtgcacaa tggtgtcctg aacgtacagc gacaccttcc atatccagag cagtcgccac
     2881 atcacgggga tggatgccat ccaaattaaa agcaaccacg ccagtatgat gttcaggatc
     2941 ttgcggtccg taaatggtga taccgccgat cgcttgtaat ttaggtaaaa cgtaggcaac
     3001 cagttcttgc tcataacgat gaatattctc cataccaatc tcattcaagt aatcgattgc
     3061 tcctgcaatg ttcggagtac cagcttcaaa tttccatggc agctctttcc atgaactatc
     3121 gtaaagattg acaaaatcta tcatttcacc gccgaattcg actggctcca tttgttctaa
     3181 caattgacgt ttgccatata atacaccgat tcctgtcggc ccgcacattt tatgcccgct
     3241 aaaggcgaaa aaatctgcat ccaacgcttg tacatcaact gtcatatgcg gtgctgcttg
     3301 ggcaccgact ggactatcct caactacaac agcctgttca accctcagcc gtttcaagca
     3361 caacaaataa ccttctggat ctggtttgcc gacctgaaga tcatcgatcg tcacaaagga
     3421 atcaattttt tccttaatca tcggatgtgc ttcaaaaaaa tagtctagct cccgcagttc
     3481 agaattgaga ctactccgat tttccagtgc tgctgatccg ctgctcaaaa tgttcagcat
     3541 ccactggtct cccaaccgct tgacttatcc cttcataaaa ggacagttct gctgccgtcc
     3601 caacaccttg ttgatagtct gcaaaaggaa ttttatagcc gaactgctcc agtaaatagg
     3661 cttcagtaaa tagtgatgaa tactctcgta aaacatccat tcactgtcaa tcatcgtccc
     3721 atcaaaatca aaaataagtg ctttggtcat cttctttccc ccttacccaa aacaagcaaa
     3781 agctgtgtcc tgctctctct acagtatcag cataaaaact gccgtctgaa aagtcaaagg
     3841 tgaaagaaat gtggcgctat aatttctagg actgatagta aatcattcta tcagtccttt
     3901 tgagcataaa aaaacaaaac atacagaagc tccgttagaa ttgaatcacc acaacacaat
     3961 caaaggagcg ttctgtatgc ctgatcttca tcttatcaaa ctattactag gaatcaaaga
     4021 tggaaacatt caaatcactc atgtcgagaa gaaaaaaata cattcaatca acagtatcgt
     4081 tgtttctgcc acgctcatta aaacgattca gccctgtcct cagtgtaaac aggtgaatca
     4141 acatgggctg atcgttaaga acggaaagaa aaaatcgctg gtccttctca ataagtgtgc
     4201 gaatcaacgg acttatcttg ctttggcaaa gcaacggtac ctctgtcgtg gctgtcacac
     4261 ctattttacg gcgaatacct acattgttga aaaaaactgt tttatcgcca aacaagtaca
     4321 ttataaaata ttggaggaat tgactgaaaa acaagcaatg acgacgattt ctaaacattg
     4381 cggcgtttct tggtcaaccg tttcgcgaac actcgcttct ctgctcccca tgacaaacgt
     4441 gaaaataaat tggttgcctc gctgttcatt ggtagatgaa tttcgttcat tgaaaaatca
     4501 agtcggtccc tattcttttt cttgtatgga tggcgatact gggaaattac tggatattct
     4561 tccttcacgt aagaagaaag atctcatttc ttattttatg caatttgaac gacgtgcacg
     4621 tttaaacgta aaaatactcg taacagatat gaacgcctct tatgctttgc tgataaaaga
     4681 gtgctttcct catgccaaac tcgttgtgga tcgctttcac atcgtcaagc acctcattcg
     4741 cagttttaag gatatttgac tatgagtcat gaaaagcttc gaccgaaacg atccaataca
     4801 agccaaacac tatcgacaag tgaaggcttt atctcgactt ctgatcaaac gacaggacac
     4861 gcttgtttat gataaatgga caaaatggcg taattttggt tgggcctatt taaccgaaag
     4921 tgaagtcgta gaacgtctac tttctacttc tgatgaacta cgaatagctt atgcttacta
     4981 tcaagaaatt cttcaagctt tttatgacaa agaagcggac atcttctttc agcttgtaaa
     5041 aacaatgccc aagtcggttc caagagaact gcatcatata aaaaaagcgt ttataaacta
     5101 tgaatccggc attcgtttag ccttagagtt gccttactca aatggcaaaa tagaaaatct
     5161 gcatacccac atcaaagcat tgaaacgaat agcttacggg ttaggatgag aaccagaatt
     5221 ttcttaatca atcatctgat cacttattaa acataaaaaa acatctaaag cagaaaatca
     5281 attctacctt agatgttctt atatcactct atcagtccta tttgacaaag agccagaaat
     5341 gtttgccgac tatacactag ctttgttgtg caaaatgctg agtatcaatt tccacaggga
     5401 ttgctagaat atctttcagc tgaatgtata aggcatcgct aattttctct tcccgatcgt
     5461 tcaaccgatc cactaaaatt tctttgccct ccagcgtttg aaaaacatac cgttgaatgt
     5521 tgcctaaaat aaatgattcc ttccatagaa ccggaatctt aaccccctga ggaaccggtt
     5581 caaattgaat cagctccggt cttaaataat aaatgcagcg attgccctta gcggaaaact
     5641 cgtgggtata ctgattcagc tcataccatt cgaaacgatt atagttgccg ataaattccg
     5701 caacaaatcg gctctgagga ttctgataca attctttcga ggtggaaacc tgagccaccc
     5761 gcccagcttc catcacaaaa atccgatcac tgattcgcat cgcctcttct tggtcatgag
     5821 tcacaaaaat catcgtcatc cctagcgatt gctggatagc gcgcaaatcc cgctgcagct
     5881 gttttctaat tcgggcatcc aaagcactta atggctcatc cagcagcagg actttcggtt
     5941 cagtgaccaa tgagcgagcc agtgccaccc gttgtcgttg accgccagaa agaaactgga
     6001 catttttatc tgcctgctcc gtcatatcca ccaatgatag catctcaaaa accttttcct
     6061 gaatcacctc aggactcagc ttctttacct tcaaaccaaa ggctacattt tcaaaaacag
     6121 tcatagttgg aaacaatgca tactgttgaa aaacaaagcc gatattgcga tccttcgtag
     6181 gcttatcggc aatattctca ttgtccagca caatctcacc gctctttggt tgttccaatc
     6241 ccgtgataca gcgcagcaac gtagactttc cgcacccgct gggacctagt aaagtaacca
     6301 gctcaccctt ttcaatctga atttcatcaa tagctaaaat ctgtttttca tcaaaaaaca
     6361 gattgagatc atgtatttct aaaaacattt tccgtcacaa ctcctttaag ggcttctttt
     6421 tcgactgctt ctcttccctc gtctgcttaa gggacagctg ttgcttttgt cgataagtca
     6481 gcataaaggt agttaaagca attcccacca taaacagcaa atacaaaacc atcacagcgc
     6541 tagccaaatg gccgttttcg ttcatccgcc gcagcatata gatgcgtacc gtctgaaatc
     6601 gaccgccaat caacaggttc gtcagcatgt actccccaaa aaccgaagaa aaaatcatca
     6661 gagaaactag ggtcacaccg atacggatgt ttggaaacag cacctggata ataatcgtag
     6721 acatcggggc gcccaaggtt tctgctgcat ccactaattc cttcagatta attaagcgca
     6781 tctggttgtt aagactaaga tagacaatcg gcaacgccgt aataaacaat gcacctacca
     6841 acactacaaa cattgggata ccagtttcag catacgttcg aagcaaagcc gttaccaaaa
     6901 tcactcctgg taaagcatac ggcagcagga gcagcttttc cagccaacga ttcaaacgag
     6961 ggaaatacaa atgaatccaa ataatggtgg gaatcatcaa caacagaatc gtcaccaaga
     7021 ccacacctgc taataaaagt gagcgaccca ccgcagcaat aaattccctg tccgtcagca
     7081 gctgttggaa ccattgcaag gtaaaatcac taggtaaaag cgattttgac catttagtgg
     7141 agctggcata caaaatcgtt ccaataatcg gcagcaagta atacaggaca aagcagaaca
     7201 aaatcggttt tattaacttt ttcacgtctg caccaccttg cctttctttt gaataatcaa
     7261 cattgccgtt accagcagga cacctaaagc gactgccaac gcactgccta aattaggctt
     7321 ggcgaacaca tctcccgaca ctaaagccgc aattcgaatc gttaaaagat ttacattact
     7381 gctggtcagg gcatacgctg tttcataggt ccccatgcca ttagcaaata agatgacaaa
     7441 ggtgctggca atcgatggtc gcaaaaaagg caaagccacc ttgaaccaag catacgctga
     7501 tgaagcgccc agcaattgtg cggactccag ccattccttt ttcacttcct tcatgctggg
     7561 atacaaaaac aagaccccta acggaatttg gaaataggta tacgtgatgg tcaatcccca
     7621 ccaagaatac aaattgaagt ccgctagaaa tggcaaaatc attttcagca cacctacatt
     7681 ccccaacaaa ataataaaag agaatgccag cggaatccca gcaaaattag ctgccaaatt
     7741 ggaaacaaag gtgattttct cctgcaattt agtcgataga ttcattaagc ataaacataa
     7801 aataaacgtc ccgattaacc caatcacact ggagctgaca gcaatcagta cgctgttaac
     7861 aaaggcctgt gtgtaatatt tgttgctgaa aatatcactg taattggcca atgtccactg
     7921 gttagttaaa tcactgtgca agctgcacag aaccataaaa agcagcggca gcagtaaaaa
     7981 tgcacagaag ataaaggagg caggaaccag aaccaagcgt tttttcatct ctttcatccc
     8041 ctcatcaatt cttgaatgtc ctgcatattc tctgacggtg caattcctaa taaccagcaa
     8101 aacaatggag ccgccagcag ctgtggtaca gtttgttcct tcagcttttg gcgtttattt
     8161 gaaatcaaat aaaaaggaac ttcacgatgt gcagccaagc tgccgccatg cagtccgtgc
     8221 gcatccatcc cgtgatccgc agtcaccacg acttgataac ctttttgcag ccattcagga
     8281 aggtaaatag ccaataatct gtccgcctga ttaaccgctg ctgcatattc atgagaatcg
     8341 gcggtaaatt tgtgaccgac atcatcaata ttcatcgaat gtacgaccaa aaagtctggc
     8401 tgatactgat tcactaaata atgggcatcc gcaaataaat gcgaatcagg gtaagtgtcc
     8461 tcactataaa agatgccatg ctgaatcagt cgctcctcct gatgctggat gcgatcaacc
     8521 aaacgattat agggggcttg attgtacaac tcgctgtacc agtggtaagc ggcggctcct
     8581 gtttttccgc cagcttcctt aactaattca aacagcgacc actctttgga tcgctgagca
     8641 tggtaattac tatagatgcc attttcatag gctggaacgc cggtcatcag tacttcgtat
     8701 aacggacgtg aattgctggg cagctcactt tttaccttca ataagcttgc ttgctggtgc
     8761 tcaactaaat gattcatgaa cccaagctgg gcgacggctg tgtcgtagcg acagccgtct
     8821 agtataacta caatcagttt gtcaagtcta ctttgcatag gtcagtacct ccgcctgcca
     8881 aagctctgga agctctacag aggtcttgtt ccaagcttca ttgtcttcaa ctggcaccgc
     8941 cgcttcataa gcatcctccg gcagtaattt ttcagctact tctttaggca gctctacctt
     9001 gcgaataggt cgcgcatagc cctttgccaa attgatttgc ccttcatcag ataaaatata
     9061 ttcccgtgct agctttgccg cattgggatg aggggcattt ttattgatta aggttgtata
     9121 cccgctaatg atggagccgt ctgttggcac agttacctca aaacgctcgg agtcaatttg
     9181 gtcacgataa tttagtccat tgaagtccca aacaactgcc acatcgattt cacctttttc
     9241 aagatttgct acgctggcat caacggtgga caaacggcct ttttctgcca tttttttgta
     9301 gtaattcaga cctggctgaa tatccttttc gtcacctccg ttggcaatcg ccgcagccaa
     9361 tacagcaaat tgtgcttggt tggcaatcgt cacatccccc acgctaactt gatagtcacc
     9421 ctcttgcagc tccttccagc tagtcggtgc ttgcttcaca ttctctttat cggtgataaa
     9481 ggaaatggtt cccgtatagc ttaacaacca atccccatca tcatcctttg cccaatccgg
     9541 aatttcctcc caatagttgg tcttataagg taaggttaac tctttgcttt cagccaacgg
     9601 accgaagctg ataccgacat caccaatatc ggcggttcca ttttttcctt ctgattcaaa
     9661 cttagccaat tcttctgcac tggacatatc cgtatccacg tgacttaagc cgtattcttt
     9721 ttcaaggtcg ttccaagtgc ctacccaatt tgcccaagta tccggcattc ccaagctggc
     9781 aatctctcct tcttccttgg ctttttctat aatcgtatct aaagagtcgt ctgccgcagc
     9841 cgattgcttc gttgcttcac ctgagccgca gcctgtcagc aggatactta ctgttgctgc
     9901 acaaaataaa ccgatagtca tgttacgaat tttttcattc attttcattc attcttctcc
     9961 cctttcgata attccattct aacaaagctt tgtaatgaga gaatcagttt cttgaaaagg
    10021 tttcatgaac aatgtaaagg ttgtatatgg tttgtaaatt tcgacgaatt cccgttaacc
    10081 ttctaaagct tttgcggtat gatagtagaa aaaaggaggc tccctatgca gtattatgat
    10141 cagcatctcc acacgtattt ttcctttgat tcagaggaaa aatttgaaaa ctatttagcc
    10201 tatcagccgg agtttttcgt ttctacggat cactttgatt taaacaatcc atctaccgga
    10261 aaagatgaca ttccggatta tccagcctat ataaacaaac tggactcttt aaaagaaacc
    10321 tattccacca aatttctgcg aggtatcgag attggcgttg ttccaggaca agaagagcgg
    10381 atcgtggatt acctcgctca gcatccttat
//
LOCUS       DASHHB010000079        10251 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.083, whole genome shotgun sequence.
ACCESSION   DASHHB010000079 DASHHB010000000
VERSION     DASHHB010000079.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 10251)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10251)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10251
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.083"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(460..1089)
                     /gene="udk"
                     /locus_tag="U2K71_002492"
     CDS             complement(460..1089)
                     /gene="udk"
                     /locus_tag="U2K71_002492"
                     /EC_number="2.7.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine kinase"
                     /protein_id="HEM7316513.1"
                     /translation="MTKSKPIIIGVTGGSGSGKTSVSRAIFNNFPDHSIMMLEQDSYY
                     KDQSHLSFEERLNTNYDHPFAFDNDLLIQHVGDLLNYKAIEKPVYDYVAHTRSQATII
                     QEPKEVIILEGILILEDERLRDLMDIKVYVDTDDDIRIIRRIKRDMEERGRTLDSVIE
                     QYLTVVKPMYHQFIEPTKRYADIIVPEGGENHVAIDLITTKVASFLNHK"
     gene            complement(1173..1868)
                     /locus_tag="U2K71_002493"
     CDS             complement(1173..1868)
                     /locus_tag="U2K71_002493"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YjjG family noncanonical pyrimidine
                     nucleotidase"
                     /protein_id="HEM7316514.1"
                     /translation="MKYKTLLFDVDDTLLDFQLTEANALSALFAEQGITLTPEIETSY
                     KALNHHLWQEFEKGRLSREEIIGSRFGLLFQQFGKTVDSDQMEKRYRHYLNQGHDLVP
                     GSLEILKKVHPHADLYIVTNGVSHTQHRRLTDAGMLDYFKDIFVSEAVGAQKPMKEFF
                     DHVFSHIPNFDKKQTVIIGDSLTSDIKGGNMAGIDTIWFNRNKLPEIEEIQPTYRIDS
                     LEELYSLLEIEKD"
     gene            complement(1881..2597)
                     /locus_tag="U2K71_002494"
     CDS             complement(1881..2597)
                     /locus_tag="U2K71_002494"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HEM7316515.1"
                     /translation="MRLDKLIEEKLETTRKEMKRLFALKQVMIDGTVEFRQNRNVDSL
                     LHQIEVAGEHLETNESYYLLNKPEKAVTAVSDPEKTTVIDLIASADRFQPLYPVGRLD
                     RDTTGLLLITSNGQLGYEMLLPEKKVSKTYQVVVNERVTPADVTAFENGIVFADGYQC
                     KPADLKILKADKHQSTVLLTIQEGKFHQVKKMFLAQGKKVTALKRLSMGPLELDEQLG
                     VGEYRSLTLAEMKKLSIYFK"
     gene            2927..3532
                     /locus_tag="U2K71_002495"
     CDS             2927..3532
                     /locus_tag="U2K71_002495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HEM7316516.1"
                     /translation="MSVSLEVSKREVRPRSLRNKLRHEGKVPAVVYGYKVESTPISFD
                     GLTLSKLLREHGANTVITMDIDGQKVNTLVHKIQTNTFTNHFEHAEFLSVNMSEETEV
                     ETEVVLVGESVGAKSGGVLAQNLYTVVVSATPDKLPERIEVDVTNLELGDSITVADLP
                     KNDDYKFVTDSEEQIAAVVAQSEEESTGEGGEPEVIGSTEE"
     gene            3857..4180
                     /locus_tag="U2K71_002496"
     CDS             3857..4180
                     /locus_tag="U2K71_002496"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF960 domain-containing protein"
                     /protein_id="HEM7316517.1"
                     /translation="MFESFDSSRNRYASVGVVSSLPDELIDSIWFIIDLDLKGVIPLE
                     NILTFDLIENRGKVTMHFSQAGSNVEMDIDLPFAYSNQFPAQVYAYDDGTRETILLPS
                     EIRQY"
     gene            4362..5732
                     /locus_tag="U2K71_002497"
     CDS             4362..5732
                     /locus_tag="U2K71_002497"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HEM7316518.1"
                     /translation="MRDLTKGSPAKLILMFTVPLLIGNVFQQFYNMIDMIIVGQTLGK
                     NALAAVGATGSLTFLIIGFAQGLTAGLAIITAQRYGAKDYRGLKKSFAASVVISLVVT
                     VILTVLSLLFIRPMLQLMQTPPEILDQAQTFISIILLGIFASVSFNLLSNVIRALGDS
                     RTPLFFLIIAVIINVVLDLIFIIYFGMGVEGAAIATVIAQVSSSVLCLVYIKKKMPLL
                     QLRKKDFKFEKEEFAVHLNAALPMAFQSSIIAVGAIVLQAALNSLGTDVVAAQAAAGR
                     IDQFANQPMMSFGIAMATFTAQNYGAKEYGRILQGVRQTLLMSVGFSLLAGAIVIFFG
                     HFFVQLFVSPSETRVFELAQTYFNINGSLYWILAVLFILRYTLQGLGQSKIPTIAGMM
                     ELLMRSFAAIVLTGMWGYPGAAAASPLAWIGSVAVLLYSYMRSMKQLKHMGEEEHFTL
                     EHAEAR"
     gene            complement(5862..7163)
                     /locus_tag="U2K71_002498"
     CDS             complement(5862..7163)
                     /locus_tag="U2K71_002498"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HEM7316519.1"
                     /translation="MVINFIILFLMIAITSFFVASEFALVKIRRSRLEQLEKENVKNA
                     KLALHVTHHLDNYLSASQLGITLTGLIIGWVGEGSVAALLEPVIGKLPFSSAISSTIS
                     VALGFILVTYIDVVIGELLPKSYSIVNTEKVVLFVVKPLHYFYKVMFPFIWVLNHSAA
                     GLGKLLGVRLVSEGEETLSQEELTLVALNSYEKGELTKEEYHYLENVFEFDDTLAKDI
                     QVDRTSMQVFEASETVKEAIKASIEQGHTRYPVILESKDNVLGYVTLPDLIKQSYKDD
                     QLTVEQLIEEPIVTTETIPIKKLLTIMRKKGKHIAILKDEYGGTSGLVTIEDILEEIV
                     GEIRDETDLDEALIAEQSDGSYIISGKLTLDDFQRYFHVEIPEFEETNFTTLAGFASS
                     RYKEIKAGTIIEIASFRFTVLEYQHAHIDYFKVESTERKIE"
     gene            complement(7393..9351)
                     /locus_tag="U2K71_002499"
     CDS             complement(7393..9351)
                     /locus_tag="U2K71_002499"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KUP/HAK/KT family potassium transporter"
                     /protein_id="HEM7316520.1"
                     /translation="MAGLLVAMGVVYGDIGTSPLYVMKSIVEDNGGLSGINPDFVIGS
                     VSLIFWTLTLLTTIKYVVIALNADNHGEGGIFSLYTLVRKGGKYLIIPAMIGGAALLA
                     DGVLTPAVTVTTAIEGLRGIPQFFDRFGNNQNVIVIITLVIIFVLFMVQRFGTDIVGK
                     AFGPIMFAWFTFLGVMGLMNFSQDWTVIRALNPYYAIQLLFSADNKLGLFILGNIFLA
                     TTGAEALYSDLGHVGKRNIRLSWPYVKICLVINYFGQAAWLLNVYQNPEAQQIKNLNP
                     FFQMMPQSWIVIGVAFATVAAVIASQALITGSFTLVSEAIKLKLLPRLKIMYPGNSIG
                     QMYIPAVNLILWIACSLVVVTFRTSHHMEAAYGLSITVTMLMTTALLYFYLIQNGHSR
                     WLAYLVTFFFGAIEVVFFISSVVKFFRGGFVAVLIGLIILVVMFIWEQGNIIRESSTE
                     DVSLRDYIPQLRELRDDTSLPIYQTNVVFLVPDMAEDKVGRQFIYSILDKRPKRARVY
                     WFVHVEVTDEPYTKEYQVDMMDTDFIVQVNLFLGFRVQQEINVYVRQVIHDLMKQGRL
                     PKQPQRYSLTPGREVGDFQFVLIDEVVSNVTTLGKWQRQIMQAKLAIKKIATSPETWF
                     GLEYSEVKHETVPLIIGNTRKTWLKERL"
     gene            complement(9528..9794)
                     /locus_tag="U2K71_002500"
     CDS             complement(9528..9794)
                     /locus_tag="U2K71_002500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HEM7316521.1"
                     /translation="MLNFLWSLFIGGILGLIAGAILGKEVPGGVSGNIIIGFLGSWLG
                     EFLLGPLGPELGGFYLIPALLGAIFCLALYSFVARRIRERPKKE"
BASE COUNT         3008 a         2120 c         1867 g         3256 t
ORIGIN      
        1 gggggtgcga caaaagagct tcttcaaaaa aataagtcga aatctcacac aaattcgaga
       61 aataattttc gtaagtttct tcttatttat tgcatctgac cacttctgtt acaacctctc
      121 gttaaacggt gttcaaaagc ttgcatctac gataataagc ccaacccaac gaaaaacgtg
      181 aggagctgtt ttatttggat gctcttatcg cttgatgcaa agacagcttt tttacaacct
      241 ctacttcacg gtgctcaatc agctgacctt cggtattagc tcaaaaaacg gtaaaatata
      301 aacagctatt ttccgtttct tctttttaat acttaggtca agatgctgtt ttcacaacct
      361 cttgttataa ataaaaaaaa cagttggctg gtgactgttc aaaaagcaga atccttgctt
      421 ttcaaacggt cgcagtccgt tcaactgttt cgctgcgcac tatttatggt ttaagaaact
      481 agctactttt gttgtaatca aatcaatagc aacgtggttt tctccgcctt ctggaacaat
      541 gatatccgca tagcgcttcg ttggttcgat aaattgatga tacatcggtt taacaactgt
      601 caaatattgt tcgatcacgg agtccaatgt gcgtccccgt tcttccatat cccgtttgat
      661 acgacgaatg atacgaatgt catcatctgt atctacgtat actttgatat ccatcaagtc
      721 acgcagacgt tcgtcttcta aaatcaggat tccttctaga atgatcactt cttttggttc
      781 ctgaatgatc gttgcctgac ttcttgtatg cgctacatag tcatacacgg gtttttcaat
      841 ggctttatag ttcagtaagt cgcctacatg ctgaatcagc agatcattat caaaggcaaa
      901 tgggtgatcg taatttgtgt tcaatcgttc ttcaaaactc aaatggctct gatccttgta
      961 ataagaatct tgttcaagca tcatgatgga atggtccggg aagttgttga aaatcgcacg
     1021 gctgacactt gtctttccgc ttccggatcc acccgtcaca ccaatgataa ttggcttgct
     1081 ttttgtcatt gactaaaacc tctttcgtat ccgttttttc attctaatct attatagcga
     1141 aaaaacacga aaatgctata tttttctgaa aattaatcct tttcaatttc caataatgaa
     1201 taaagttcct ctaatgaatc gattcgataa gtaggctgga tttcttcgat ttctggtaat
     1261 ttatttctat tgaaccaaat cgtatcgatc cctgccatat ttcctccttt gatatcagaa
     1321 gtcaaagaat caccaatgat cacagtttgc tttttatcaa aattaggaat gtgggaaaag
     1381 acatgatcaa aaaattcttt catcggcttt tgcgctccga cagcttctga gacaaagata
     1441 tccttgaagt aatcaagcat tcctgcatct gttaatcgcc gatgttgcgt gtgcgacaca
     1501 ccgttcgtca caatataaag atcagcatgc ggatggacct ttttcaggat ttctagactt
     1561 cctggaacaa gatcatgacc ttggttcaag tagtggcggt agcgtttttc catttgatca
     1621 ctatctactg ttttcccaaa ttgctggaaa agtaacccaa aacgactgcc tattatttct
     1681 tctcgtgata gtctgccttt ttcaaactcc tgccataaat gatgattcaa agccttatag
     1741 ctggtttcga tttctggtgt caatgtgatt ccttgttctg caaacaaagc acttaaagca
     1801 ttggcttcag ttaattgaaa atctaacaat gtatcatcta catcgaacaa taaagtttta
     1861 tatttcatct ttttgacctc ctatttaaaa taaatactta actttttcat ctctgctaat
     1921 gtcaacgatc gatactcgcc tactcctagc tgttcatcta attccaatgg tcccatgctt
     1981 agccgtttca atgcggtcac tttctttcct tgggctaaga acatcttttt gacttgatgg
     2041 aattttcctt cttggatcgt caataaaacc gtactttggt gcttgtctgc tttgagtatt
     2101 tttaaatcag ccggcttaca ttgataccca tcagcgaata cgattccatt ttcaaatgct
     2161 gttacgtctg ccggtgtcac cctttcgttc acaactactt gataagtctt tgaaaccttt
     2221 ttttctggaa gtagcatttc atatcccaat tgtccattcg aagtgatcag caatagtcct
     2281 gttgtatctc tatctagtct tcccactgga tataatggtt ggaaacgatc ggcacttgca
     2341 atcagatcga ttactgtcgt cttttctgga tctgatacag ctgtaacagc tttttcaggt
     2401 ttattgagaa gataatagga ctcatttgtt tctaagtgtt cacctgctac ttcgatttga
     2461 tgcagtaaac tatctacatt tcgattttgt cgaaattcga ctgtgccatc tatcatcact
     2521 tgcttcaaag caaacagccg cttcatttcc ttgcgtgtcg tttctaattt ttcttctatt
     2581 aatttatcta aacgcatgct gtttcctttc ttattactac taattaatta taccttgaat
     2641 attcttgctt gcaaatacat catcatagtt attccttgac ttaattaaca gttcacgtta
     2701 cggttaaata gtaaagtgaa ttggatttct catacacagc gtaatgacct ggtggagaaa
     2761 agttgaatcc tgactttcta aggggagatg cgcgcatctc tccttttgcg cttttttaag
     2821 cgccctgcct tcgatgatgt gcacgagatt agcctttaga attcacttaa ataaaatctg
     2881 tccgttacca aagcagacag tcacatttta aggaggaatt ttggttatgt cagtatcatt
     2941 agaagtaagt aaaagagaag tacgtccccg ttcattaaga aacaaacttc gtcacgaagg
     3001 gaaagttcca gctgttgttt atgggtataa agtagaaagt acccctatct cattcgatgg
     3061 tttgacacta agcaaattgt tgcgtgaaca tggagcgaac acagtcatca cgatggacat
     3121 cgatggacaa aaagtcaata cattggttca caagatccaa acgaacactt ttaccaatca
     3181 tttcgaacat gcagaatttc tatctgtaaa catgtcggaa gaaacagaag tcgaaacaga
     3241 agtcgttctt gttggcgaat cagtgggtgc taaatctggt ggagtgttag ctcaaaactt
     3301 gtatacagta gtcgtatctg ctacaccaga caaattgcct gaacgaattg aagttgacgt
     3361 gacaaacttg gaattaggtg attctattac agtagccgat cttccaaaaa acgacgatta
     3421 caaattcgtg accgacagtg aagagcaaat cgctgctgta gtcgctcaaa gtgaagaaga
     3481 aagcacaggt gaaggtggcg aaccagaagt gatcggttca acagaagaat aatactgaaa
     3541 caatcaggtg gtgttagaag cggcgggtca gcagctttta acacgccttt tttatttcta
     3601 atcgtttatt ttcatatggg gcttccactt ttgtaaatgg ctttacaaac gaaaaatcct
     3661 aatttaccaa gcttttcctc gcatactttt atcgtacctt tataaaaatt tatactttga
     3721 aaaaaagtat tcaattattg ttgcattttt cctgattctt tattagaatg aaccctcgtt
     3781 gaagtaagta tttctagttt atcctaggat taattataaa aataaaacat tcgttgattc
     3841 gaaggaggtt aaacagatgt ttgaaagttt tgatagcagc cgcaaccgtt acgcttcagt
     3901 tggtgtcgta tcaagtttgc cagatgaatt gattgacagt atatggttta ttattgacct
     3961 agatcttaag ggagtcattc cattagaaaa tatattaacc tttgatttga tcgaaaatcg
     4021 tggaaaagtg acgatgcatt tttctcaagc aggaagcaat gtggaaatgg atattgattt
     4081 gccgtttgcc tattcgaacc aatttcctgc tcaagtatac gcctatgatg acggtacgag
     4141 agaaacgatc cttttaccaa gtgagattag acaatattaa ggtgatagat aaacgtggat
     4201 gtcccttatt ttacagacca ggccaagttg ttgattcttt gactcaattc ttggcttctt
     4261 tttatggagt ttcataaatt atcattgatt tctaattggt attctcagga taaacgctat
     4321 atactactaa ctaaatttta aagatataag gagtttaaaa gatgcgcgat ttaacaaaag
     4381 gaagccccgc taaactgatt ttgatgttca ctgttccttt actgatcgga aatgtttttc
     4441 agcagttcta caatatgatc gatatgatca tcgtgggtca gacgctaggt aaaaatgcgc
     4501 tggcagcggt tggtgcaact ggaagcctga catttttgat cattggcttt gcccaaggtt
     4561 taacagctgg tttagccatt attaccgcac aacgttacgg tgcaaaagat tatcggggtc
     4621 tgaagaaaag ttttgctgct agtgttgtca ttagtcttgt cgtcactgtg attttaactg
     4681 tcctaagtct cctgtttatt cgtccgatgc ttcaattgat gcaaactcct cctgaaatac
     4741 ttgatcaagc acaaacattt atttcaatta ttttattagg gatctttgca tcagtcagtt
     4801 tcaatttgct ttctaatgtc attcgcgcac ttggtgacag ccgcacacca ctattctttc
     4861 tgatcattgc ggtcatcatc aacgtcgtac ttgatttgat ctttattatc tattttggca
     4921 tgggtgtgga aggtgcagct attgctacag taatcgcaca agtttcttcc agcgtactgt
     4981 gtctagtgta tatcaagaaa aaaatgcctt tgttacagtt acgcaaaaaa gatttcaaat
     5041 ttgaaaaaga agagtttgct gtacacctaa atgctgcttt gccaatggcg tttcaatctt
     5101 cgatcatcgc tgttggtgct atcgtgttac aagcagcact aaatagtcta gggacagatg
     5161 tcgtagctgc tcaagccgct gctggtcgta tcgaccaatt tgcaaatcag ccgatgatgt
     5221 catttggtat tgcaatggca actttcactg cacaaaacta tggtgcgaaa gaatatggcc
     5281 gaatcttgca aggggtacgt caaactttac tgatgagtgt cggatttagt ctcttagcag
     5341 gtgctatcgt cattttcttc ggtcatttct ttgttcaatt gtttgttagc ccatcagaaa
     5401 cacgtgtatt tgaattagca caaacctact tcaatatcaa tgggtctctt tactggatct
     5461 tagcagtcct gttcatttta cgctacacac ttcaagggct tggacaaagc aagatcccta
     5521 cgatcgccgg tatgatggaa ttgcttatgc gttcttttgc agctatcgtt cttacaggaa
     5581 tgtggggata cccaggagca gcagcagctt ctcctttagc ttggatcggt tctgttgccg
     5641 tgttgcttta ctcttatatg cgttcgatga aacaattgaa acacatgggt gaagaggaac
     5701 atttcacatt agaacatgca gaagcaagat agccttgatt aaaagaaatc gttgcggcta
     5761 aaaatgaaaa cttttattga aaatatcttc ttcgtttacc gaaaacaggt tttttagata
     5821 acaaaaaggc tgtgacaaaa aatgtcacag ccttttttct tttattctat ttttctttcc
     5881 gtggactcta ctttgaaata atcgatatgt gcatgctggt attctaatac cgtgaaacgg
     5941 aatgatgcaa tctcaatgat tgttcctgct tttatttctt tatatcggct agaagcaaag
     6001 cccgcaagtg tagtaaaatt cgtttcttca aactctggaa tctctacatg aaaataacgc
     6061 tggaaatcat ctaatgttag tttgccggag atgatataag agccatctga ctgttccgct
     6121 atcaatgctt cgtccaaatc tgtctcatct cggatttccc cgacgatttc ttctaaaata
     6181 tcttcaatcg tcactaagcc gcttgtccct ccgtattcat cttttaagat agcaatatgc
     6241 tttccctttt ttcgcatgat cgtcaataat ttttttatag ggatcgtttc agttgttacg
     6301 atcggctctt caatcaactg ttctactgtc agctggtcat ctttataaga ttgcttgatc
     6361 aaatctggca aggtgacata acctaaaaca ttgtcttttg attctaagat aaccggatag
     6421 cgagtatgtc cttgttcaat cgaagctttt atcgcttctt tgacggtttc tgaagcttcg
     6481 aatacttgca tggacgtacg gtctacttgt atatcttttg ccaaggtatc atcaaattca
     6541 aacacgtttt ccaaataatg atattcttct ttggtcaact ctcctttttc ataagaattc
     6601 aatgcgacta atgtcagctc ttcttgagac aatgtctctt ctccttcaga tacgagacgc
     6661 acacctaata gcttccctag ccctgcagct gaatgattca atacccaaat aaaaggaaac
     6721 attactttat aaaaataatg aagtggttta actacgaaca agacgacctt ctccgtattg
     6781 acgatactat agctcttagg aagcagttcg ccaataacca catcgatata cgtaactaaa
     6841 ataaatccca gcgccacaga gattgtactg ctaatagccg aactaaaagg tagtttaccg
     6901 atcactggtt ctagtaaagc cgcgacagat ccttcaccga cccacccgat gatcaaaccg
     6961 gttagcgtaa ttcctaattg actagctgat aaatagttat ctaaatggtg tgtaacatga
     7021 agagctagct tggcgttctt tacattttcc ttttctagtt gttccaatct tgatcgtctg
     7081 attttcacta aagcaaattc acttgctaca aaaaatgaag taattgcaat catcaaaaat
     7141 aaaataatga aattgataac catattctcc cgcctttctc tttttctatt gtaccctttt
     7201 tctacaaaaa gattgaaaat aatgcgtatt catatttagg aataaaaaag gagatgcggc
     7261 atttttcagc cacatctcct tatcacttca aaatcattgc gttacttgtt gctaaacagc
     7321 tttttcatga cctctgataa acggtgttca agtagctgac attcgacatt agttcaggtc
     7381 aaagcgctac tttcacaacc gctctttcaa ccatgttttc cgagtatttc caatgataag
     7441 cggaacagtt tcatgtttta cttcgctgta ctctagccca aaccatgttt ctggcgaggt
     7501 agcgattttt ttgattgcca gttttgcctg catgatttga cgttgccatt ttcctagagt
     7561 tgtcacattg gaaactactt catcaatcag gacaaattgg aaatcgccaa cttctcgccc
     7621 tggtgtcaga gagtatcttt gtggctgttt tggcaaacgg ccttgtttca tcaaatcatg
     7681 gatgacttgg cgaacatata cgttgatttc ctgttggacc ctgaaaccta agaacagatt
     7741 cacttggaca ataaaatcag tgtccatcat atctacttga tattctttcg tatatggctc
     7801 atccgtcact tccacatgaa caaaccaata gactcgtgct cttttaggcc gtttatctaa
     7861 aatcgaatag atgaactgtc ggccaacttt atcttcagcc atatcaggaa ccaagaagac
     7921 cacatttgtc tgatagattg gtaaagaagt atcatcacgt aattctctaa gctgcgggat
     7981 ataatctctc aaagacacat cctcggttga cgattcgcga ataatgttcc cttgttccca
     8041 aataaacatc acgactaaga tgatcaaacc aatcagaacg gctacaaaac cgcctcggaa
     8101 gaatttgaca acacttgaga taaagaaaac gacttctatc gcaccgaaga aaaaggtaac
     8161 taaatatgca agccaacgag aatgcccatt ttggataaga taaaagtaaa gcagtgctgt
     8221 tgtcatcaac atcgtcacag tgatcgatag accgtaagca gcttccatat gatgagacgt
     8281 tcggaatgtg acaaccacta gagaacaggc gatccataaa atcagattga ctgctgggat
     8341 atacatttgc ccgatactgt tcccaggata catgatcttt aaccgaggca gcagtttcaa
     8401 cttgattgct tctgaaacta gcgtaaacga acctgtaatc aatgcttgcg aagcaatgac
     8461 tgcagcgact gttgcaaaag caacgccgat cacgatccaa ctttgaggca tcatttggaa
     8521 gaatggattc aggttcttga tttgctgtgc ttcagggttt tgataaacat tcaacaacca
     8581 cgcagcctgc ccaaaataat tgataaccaa acaaattttg acgtaaggcc agctcagacg
     8641 aatatttcgt ttgccgacat gtccaagatc ggaataaagc gcctctgcac ctgtcgtagc
     8701 caggaaaata tttccaagaa tgaacaagcc taatttgtta tctgcactaa aaagcagctg
     8761 gatagcataa taaggattca gtgcacggat aactgtccag tcttgactga agttcattaa
     8821 tcccattacg cctaaaaaag taaaccaagc gaacatgatt ggtccgaatg cttttccgac
     8881 aatatctgta ccaaagcgtt ggaccatgaa taaaacaaaa ataataacta aagtgataat
     8941 gacgatcacg ttctggttat taccgaaccg gtcaaaaaac tgcggaattc ctcgcaagcc
     9001 ttcaatcgca gtcgttaccg tcacagcagg ggtcaatacc ccatctgcta gtaaggcagc
     9061 tcctccgatc attgcaggaa tgatcaaata tttcccacct tttcggacaa gtgtgtagag
     9121 tgagaaaatt ccaccttcac catggttatc tgcattcaat gcaatcacga catatttgat
     9181 tgttgtcagt agagtcaatg tccaaaaaat caatgagact gatccgataa caaaatcagg
     9241 attgattcct gacaatccgc cattatcttc aacaatcgat ttcataacgt aaagcggact
     9301 tgtcccgatg tctccgtaga cgactcccat cgctacaagc aggccagcca ttgatatctt
     9361 tttggtattg tgcgttgttg ccacgttttt ccctcatttc ttgttatatg gatatccttt
     9421 aggtaagctt gaatgataga tgatattctt atccatgaca cccgctttcc taaattgctt
     9481 ctaattatcc aagtaacaca ctgaagtgtc aacttatttg aataaaatta ttctttcttt
     9541 ggacgttctc tgatacgtct tgccacaaat gaataaagag caaggcaaaa aattgcaccc
     9601 aataatgctg gtatcaaata aaaaccgccg agttccggtc ccagtggtcc tagtaaaaat
     9661 tcgccgagcc aactacctaa aaaaccgata atgatatttc cagatacgcc tcccgggact
     9721 tcctttccta aaattgcgcc agctatcaaa cctaatatac cgccgataaa taacgaccat
     9781 aaaaaattca gcatctcctt tccctccttt atccatattt ctcactttta ttttacgctt
     9841 tattcagaag gactccaaaa atatgcgcca agaaactcca gaaaaaaaga agttgtaaca
     9901 aaagtgttca gcttatttcc gtagaagctg cttctggaac accgtgaatc agaggttgtg
     9961 aaatcagcgg tttgacctga gtattagaga aacaaacgga aaaatagttc ctcatatttt
    10021 gccgtttttt gagctaatat cgagggacag ctgattgaac atcgtttatt ggagaggttg
    10081 taaaaaagct gttctgcatc aagtaataag aacagccaag taaaaacagc tcctaatgtt
    10141 tttaacttgg ctggacttat taccgcagat gcaagctttt gaacaccgtt tattcgaaaa
    10201 aaaaggactg tgacacggct tttgtcacag tccccttttt tcgaataatt a
//
LOCUS       DASHHB010000080         9886 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.084, whole genome shotgun sequence.
ACCESSION   DASHHB010000080 DASHHB010000000
VERSION     DASHHB010000080.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9886)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9886)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9886
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.084"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(109..2229)
                     /locus_tag="U2K71_002501"
     CDS             complement(109..2229)
                     /locus_tag="U2K71_002501"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LTA synthase family protein"
                     /protein_id="HEM7316522.1"
                     /translation="MKCIKTPAFLNKRLGFFSLLVVLLWLKTIFAYLVDFHLGIESGI
                     QYFILFINPIATTLLLLGIALYIRRPRVAYITMMVIYFLLTLLVFSNVTYYREFTDFI
                     TINTILGAGKVASGLGESAIRLFRPYDVIYWIDFIILTVLLIAKKIKLDPRPIRARMA
                     FAISTLAVMIFSGNLFMAEADRPELLTRTFSRDYLVKYLGLNAFMVYDGVQTYQTNQV
                     RAEASPNDMKEVENYVKEHYAAPNDDLFGLARGKNVIYIHLESFQQFLIDYKLTDENG
                     LQHEVTPFINSLYHSNSTFSFDNFFHQVKAGKTSDAETLMENSLFGLNQGSLFTQLGG
                     KNTFQAAPDILSQTQGYTSAVFHGNAGTFWNRNETYKNLGYDYFFDSSYYDVNDDNSF
                     QYGLHDKPFFQQSVQYLERLQQPFYSKFIAVSNHYPYSKFTNDEAGFPLANTNDETIN
                     GYFATANYLDTAVQEFFNYLKESGLYDNSVIVLYGDHYGVSNSRNKNLAELVGKTSST
                     WTNYDNAQMQRVPYMIHIPGQTQGGINHTYGGQVDALPTLLHLLGVDTKNYIQLGQDL
                     FSKDHEQLVSFRDGDFVTPKYTYYGGTIYSNETGEAITEPTEEVQNEIDSLKEKVDKQ
                     LAVSDQINNGDLLRFYTNSGLDPIDTSDYDYKNGLEKLKEAEKSLGDKSTSVFSEHNG
                     QSTVDLYKTQTYQELHGTTTSSSE"
     gene            2459..3637
                     /locus_tag="U2K71_002502"
     CDS             2459..3637
                     /locus_tag="U2K71_002502"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent rRNA methyltransferase"
                     /protein_id="HEM7316523.1"
                     /translation="MKVKVKKRASNRLKKGYPLIQKEDLAHPQKETTQWVELVDSQNQ
                     FIAKGYLGKQNKGIGWVLSQQNIPFDQSFFEEFFVKSKEKRTFFFSDDSTTAFRIFNG
                     EGDGIGGLIVDYYAGYAVFSWYNETLYAHRELLIQAFREIYPEIIGGYEKIRFESVNL
                     PESQFIYGKEAPEPLIVKENGVKFAVYLNEGLMTGIFLDQKEVRGSLAEGFASGKTVL
                     NMFSYTGAFSVAAAMGGAIETTSVDLAKRSLKKTQEQFEVNGLDVDQQKIIVMDVFEY
                     FKYAKRKQFLYDMIVLDPPSFARNKKKVFRVAKDYGELVKDSLDILAEDGILIASTNA
                     ANVSIEQFQTMIEEEFHAKNVSFEQLALYRLPKDFQTTDTFLEGNYLKVFVYQIKNNH
                     "
     gene            3653..4411
                     /gene="aroD"
                     /locus_tag="U2K71_002503"
     CDS             3653..4411
                     /gene="aroD"
                     /locus_tag="U2K71_002503"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I 3-dehydroquinate dehydratase"
                     /protein_id="HEM7316524.1"
                     /translation="MVRVKGLNIGSGRPKICAPLIGAVREEVLREAVLAREAGADLVE
                     WRVDHYREVFSFDEVAETLVLIHEMLDGLPLLFTFRTLAEGGEQDISIRQYRDLYEHV
                     IHTGLVDMVDIELFKVESLGKRLIEEIKSLNIPLIISSHDFKETPADPVLLYRLNVME
                     HFGADIGKLAVTPNNERDVLRLMELTRRANAFVSMPIITMSMGNLGKISRLVGGTTGS
                     VMTFGSLSSDSTSAPGQLPVKALKQIIQLLNEPL"
     gene            4434..4919
                     /gene="ybaK"
                     /locus_tag="U2K71_002504"
     CDS             4434..4919
                     /gene="ybaK"
                     /locus_tag="U2K71_002504"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cys-tRNA(Pro) deacylase"
                     /protein_id="HEM7316525.1"
                     /translation="MAKKKIVKTNAIRMVEQKKIPYTEHEYEWDESHLSASSVAEQLP
                     ESQSRIFKTLVAVGNVTGPLVAVIPGEAELNLKKLAKVSGNKKVEMLHLKDLEATTGY
                     IRGGCSPIGMKKLFPTYLDQIAESYEQIIVSAGRRGLQMELAPQDICALTSGQFADIK
                     Q"
     gene            complement(4990..6243)
                     /locus_tag="U2K71_002505"
     CDS             complement(4990..6243)
                     /locus_tag="U2K71_002505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HEM7316526.1"
                     /translation="MEKLYDVIVVGAGTSGMMAAISAAEQGARVLLIEKNKKAGKKLL
                     MTGGGRCNVTNNRPVDDLIAHIPGNGKFLYSTFAQWNNFDIMNFFESQGVHLKEEDHG
                     RMFPVTNKSKTIIEALLNRLKELDVTLLFSTRVEKLIHKEHKIYGIRTEFEEFHAPAI
                     ILTTGGRTYPSTGSTGDGYKIVKRVGHTVTPLYATESPLISDEPYIQEKTLQGLSLQD
                     ITLRVLNKKGRVLTEHTMDLLFTHFGISGPAALRCSSFVNKELEKTGEPVTLSLDCFP
                     TQTKQELIHILTEKSKATKKNLVNAWHGILPERLLVFFLERLEMDHLTGQQASQKQIQ
                     DFVQLCKEFKLSINKTFPIEKSFVTGGGVSLKEIYPKTLESKIIDGLYFAGELLDVNG
                     YTGGFNITAAFATGHVAGMNAGQRA"
     gene            6360..7709
                     /gene="gdhA"
                     /locus_tag="U2K71_002506"
     CDS             6360..7709
                     /gene="gdhA"
                     /locus_tag="U2K71_002506"
                     /EC_number="1.4.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-specific glutamate dehydrogenase"
                     /protein_id="HEM7316527.1"
                     /translation="MTNATEYVQAIQEKLHQKDQGQEEFLQAIDEFMPTVMSFLDTHP
                     EYIEKNILELLTEPERVIQFRVPWQDDEGNWRVNRGYRIQYNSAIGPYKGGLRFHPSV
                     NLSILKFLAFEQIFKNSLTGLPIGGGKGGSDFDPKGKSDNEIMRFCQSFMLELSKHIG
                     PSIDVPAGDIGVGAREIGYLFGEYKRLKKYDTGVLTGKPLDFWGSKIRTEATGYGLVY
                     YVKHLLNEEKDSFNQKTVFVSGSGNVAIYAIEKVQELGGKVVTCSDSSGYIYDPEGID
                     VKLLKEVKEINRQRLTEYANQRPSAIYHAGESVWTLKEKADIALPCATQNEIDESLAE
                     ILVENGIKIVAEGANMPCTVKAVSVLHEAGVWYCPGKAANAGGVAVSALEMSQNVQRL
                     TWEREQVDEQLDQIMETIYHTCRQTAAEYGDEKNLLLGANVAGFEKVAKAMAAEGLV"
     gene            7821..9167
                     /locus_tag="U2K71_002507"
     CDS             7821..9167
                     /locus_tag="U2K71_002507"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HEM7316528.1"
                     /translation="MAHIHFDYSKVAPFVNEQELGYMQSQVTAAHNELREGTGAGSDF
                     RGWIDLPTNYDKEEFARIKEAAKKIQSDSEVLVVIGIGGSYLGARAAIDFLNHTFFNL
                     LDSNTRKAPQIFFAGNSISSTYIADLIEVIGDRDFSVNVISKSGTTTEPAIAFRVFKE
                     LLVKKYGQEEANKRIYATTDKAKGAVKVEADAEGWETFVIPDDIGGRFSVLTAVGLLP
                     IAVSGADIDALMQGAADASKAYSSDKLEENEAYQYAAMRNILYRKGKVTELLINYEPG
                     MQYFSEWWKQLYGESEGKDQKGIYPSSANFSTDLHSLGQFIQEGRRNIFETVVKVEKP
                     RKSITIPEQAEDLDGLGYLQGKEVDFVNTKAFEGTLLAHTDGDVPNLLVKIPEMDAYT
                     LGYTMYFFEIAVGISGYLNGINPFDQPGVEAYKKNMFALLGKPGFEDLAKELNERL"
     gene            complement(9475..9861)
                     /locus_tag="U2K71_002508"
     CDS             complement(9475..9861)
                     /locus_tag="U2K71_002508"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YxeA family protein"
                     /protein_id="HEM7316529.1"
                     /translation="MKKILGLIVIILIAFIGWKGWDYYQSTYVGKDYYAVVKAPMPAE
                     TDIKADNGEVIGRGFKYNVDAYAENGEKRQLDFDVITSGDSANGSAYPEGTILQLKAS
                     EKRIIEKKVITSDKVPSSVKEKLGLS"
BASE COUNT         3148 a         1800 c         2025 g         2913 t
ORIGIN      
        1 ctgagccaga atgaggttca ttctgaccca gatttgattt tgtacaaccc atcagtcctt
       61 attgacaaag agcctaattt tgtcgctgct ccttttaaat ttattttatc attcggaaga
      121 acttgtggtc gttccgtgaa gttcttggta cgtttgcgtt ttataaagat caacagttga
      181 ttgtccgttg tgttcactaa agacactagt tgacttgtca ccaagagatt tttcagcttc
      241 ttttaatttc tctaagccat tcttgtagtc ataatcactg gtatcaattg ggtctaatcc
      301 actattcgta tagaaccgaa gaagatcacc attattgatt tggtcagaaa cagctaactg
      361 tttatcaact ttttctttca gtgaatcaat ttcattctgt acttcctcag taggttcagt
      421 aatagcttca ccagtttcgt tactatagat cgtaccaccg tagtaagtat attttggtgt
      481 aacaaaatca ccatcacgga aagaaacaag ctgttcatga tctttcgaga atagatcttg
      541 tcctaactga atataatttt ttgtatctac acctagtaag tgaagtaatg taggcaaggc
      601 atcaacttgt ccgccgtaag tgtgattgat tccgccctgt gtttgtccag ggatatggat
      661 catataaggg acacgctgca tttgtgcatt gtcgtagttt gtccaagtag aacttgtttt
      721 tccaactaat tcagctagat ttttatttct tgaattcgag acaccgtaat ggtcaccgta
      781 aaggacgatg actgaattat catacaagcc gctttctttc aaataattga agaattcttg
      841 tacggcggta tctaaataat tggctgtcgc aaagtaaccg ttgatcgttt catcatttgt
      901 attggctaac gggaatcctg cttcgtcatt ggtgaatttt gaataaggat aatggttaga
      961 tacagcaatg aattttgaat aaaatggttg ttgcagacgt tccaagtatt gtacggattg
     1021 ttggaaaaat ggtttatcat gcaacccgta ttggaacgaa ttgtcatcat taacatcata
     1081 ataagaagaa tcaaagaaat aatcatatcc taaattcttg tatgtctcgt ttcgattcca
     1141 gaatgtacca gcgttcccat ggaataccgc actagtgtaa ccttgtgttt gacttaaaat
     1201 atcaggtgca gcttggaaag tattttttcc gcctaattgt gtgaacaagg agccttgatt
     1261 caagccaaac aatgaatttt ccatcagcgt ttccgcatcg cttgtttttc ctgcttttac
     1321 ttgatggaaa aagttgtcaa aactgaacgt gctgttgctg tggtaaagac tattgatgaa
     1381 aggtgtaact tcatgctgga gaccattctc atctgtcaat ttataatcga tcaggaattg
     1441 ctggaagctt tctaaatgga tataaatcac attttttcct ctagcgagcc cgaacaaatc
     1501 gtcatttggt gctgcataat gttcttttac ataattttct acttctttca tatcattggg
     1561 acttgcttct gcccgcactt ggtttgtctg gtaagtttga acaccgtcat aaaccatgaa
     1621 agcattcaag ccaagatatt tgaccaaata atcacgagag aatgttcgag tcagcaattc
     1681 tggacgatca gcttccgcca taaaaagatt tcctgagaaa atcataactg ctaatgtaga
     1741 gatcgcaaaa gccattcttg ctcggatcgg gcgagggtcc aacttgattt tttttgcaat
     1801 cagcagaaca gtcaagataa taaaatcgat ccaatagatc acatcatatg ggcgaaacag
     1861 tcgaatggca ctttctccca gaccactcgc tactttccca gcacctaaaa tcgtgttgat
     1921 cgtaatgaag tccgtgaatt ctcgataata agtgacattc gaaaaaacaa gcaatgtcaa
     1981 aagaaaataa atgaccatca tcgtgatata agcaactcta gggcgtctga tataaagagc
     2041 aattccaagt aataacaatg tagttgcaat agggttgata aacaagataa agtattgaat
     2101 gccactttcg attccgagat ggaaatcaac taaataggca aaaatcgttt tcaaccaaag
     2161 taaaacgact agtaaagaaa aaaatccgag cctcttattc aaaaaggcag gtgtttttat
     2221 acatttcaag ataatcttcc tttctgcact tcttaatcac agaagtgtcc tgaaaccatg
     2281 ttacacagct cacttattct catttatttt actagtatga caatatagtg tcaattgaaa
     2341 ccagaagttt atgctgtttc attaaggttt tctttaagct tcatgaaaaa atgagcagta
     2401 agacaagtcc tatgtttttt gttatacttg ggaagagaaa gctaaagcag gtgaaggaat
     2461 gaaagtaaaa gtaaaaaaaa gagcaagcaa tcgattgaaa aaaggatatc cactgatcca
     2521 aaaagaagac cttgctcatc cccaaaaaga aaccacgcaa tgggtggaat tagttgattc
     2581 gcaaaaccaa tttatcgcaa aaggatatct aggaaagcag aataagggaa tcggttgggt
     2641 attgagccag cagaacatcc catttgatca atcttttttt gaagaatttt ttgtgaagtc
     2701 aaaagaaaaa agaactttct ttttttcaga tgattctact accgcttttc gtatattcaa
     2761 tggagaagga gacgggatcg gcggtctgat tgttgattat tatgcaggtt atgctgtttt
     2821 ttcttggtat aacgaaacgt tatatgcaca tcgtgaatta ttgattcaag cttttcgtga
     2881 aatctacccg gaaatcatag gaggctatga gaaaattcga tttgaatcag taaatctgcc
     2941 tgagagccag ttcatttacg gaaaagaggc accggaaccc ttgatcgtaa aagaaaacgg
     3001 agtaaaattt gccgtttatt taaatgaagg attaatgact gggatctttt tagaccaaaa
     3061 agaagtacgg ggatcattag cagaaggttt cgcttctggg aaaacagttt tgaacatgtt
     3121 tagttatacc ggcgcctttt ctgttgctgc cgcaatggga ggggcaatcg aaacaacaag
     3181 tgtcgatcta gcaaaacgca gtttaaaaaa aacacaggaa cagtttgaag tgaatggttt
     3241 ggatgtcgat cagcaaaaaa tcattgttat ggacgtattc gagtatttca aatacgccaa
     3301 aagaaaacaa tttttatatg acatgattgt attggatcca ccaagttttg ccagaaataa
     3361 gaaaaaagtc tttcgcgtag caaaagatta tggggaactg gtcaaggata gtctggatat
     3421 tttagcagaa gacgggatac ttattgcgtc tacaaatgct gcgaatgtct cgatagaaca
     3481 attccaaaca atgattgaag aggaattcca tgccaaaaat gtttcttttg aacaacttgc
     3541 tttatatcgt ctgccaaaag atttccagac gacggatacc tttttagaag gcaattatct
     3601 gaaagtgttt gtttatcaaa ttaaaaataa ccattagaag gagcgatgca gaatggttcg
     3661 ggtaaaggga ttgaatatag gaagcggtag accaaagatt tgtgcgcctc ttatcggggc
     3721 cgtccgagag gaagtattac gtgaagcagt tcttgcacgt gaagcagggg ccgatttagt
     3781 cgaatggcga gtcgatcatt atcgtgaagt tttttctttt gatgaagtag cagaaacatt
     3841 ggttcttatc catgagatgc ttgatggact gccgttatta tttacgtttc gtaccttagc
     3901 tgaaggcgga gaacaagaca tatcgatccg tcagtatcgc gacctttatg aacatgtgat
     3961 acacacagga ttagtagata tggtcgatat cgagttgttc aaagtagaaa gtctaggaaa
     4021 aagactgata gaggaaatca agtctttaaa cattccttta attatcagta gtcatgattt
     4081 taaagaaaca ccagcagacc ctgtcttgct ttaccgattg aatgttatgg aacattttgg
     4141 agccgatatc ggaaaactgg cagtaactcc caataatgaa cgagatgttt tgcggttgat
     4201 ggagttgact agacgggcaa atgcttttgt ctccatgcct attattacga tgtccatggg
     4261 aaatcttggt aaaatttctc gtctggttgg cggaacgacg ggttccgtta tgacatttgg
     4321 ttccttaagc agcgattcta cttctgctcc aggacaatta ccggtcaaag cattgaagca
     4381 aattattcaa ttattaaatg aaccgttgta gaacaagttt gaggtgatat tttttggcaa
     4441 agaaaaaaat agtaaaaacg aatgcaatcc gtatggtcga acaaaaaaag attccctata
     4501 cagagcacga atatgagtgg gacgaaagcc acctaagtgc gtcatcggtc gctgaacagc
     4561 ttccagaaag ccaatctcgt atatttaaaa cactggttgc tgtgggcaat gtgacggggc
     4621 ctcttgttgc tgtgataccg ggagaagccg aattgaatct aaaaaaatta gcaaaagttt
     4681 cagggaataa aaaagtagaa atgctccatt taaaagattt ggaagcaacg accggataca
     4741 tacgtggtgg ctgctcgcct ataggtatga aaaaactatt tcctacttat ttagatcaaa
     4801 ttgcagaaag ctatgaacaa atcattgtct cggctggcag aagagggcta cagatggagt
     4861 tagcacctca agatatctgt gcattaacga gcggacaatt tgcagatata aaacaataaa
     4921 tcatacgtat aaaagaaaga aggaggccgg gatgttgttt gtccagtctc cttctttctt
     4981 ttttatacat tatgctcttt gcccggcatt cattcctgct acatgccctg tagcgaaagc
     5041 cgctgtgata ttaaagccgc cagtataacc attgacatcc agtaattctc ccgcaaaata
     5101 cagaccatca atgattttac tctccaatgt tttaggataa atttccttta gagaaacccc
     5161 tccgccagtt acaaatgatt tctcaatagg aaaagttttg ttgattgata gcttaaactc
     5221 tttgcatagt tgaacaaaat cttgaatttg tttttgagag gcttgttgtc ctgtcagatg
     5281 atccatttca agccgctcaa gaaagaaaac taacaatcgc tcaggcaata taccatgcca
     5341 agcattgacc aagttctttt tggttgcttt acttttttct gtcaatatat gaataagctc
     5401 ttgtttggtt tgtgtaggga aacaatccag agaaagtgtc actggttctc ccgttttctc
     5461 taattcttta ttgacaaaac ttgaacaacg cagtgcagcc ggaccagaaa taccgaaatg
     5521 ggtgaaaagt aaatccatcg tatgttcagt caatacacga ccttttttat ttaaaacgcg
     5581 taaagtgatg tcttgtaaag aaagcccttg aagtgttttc tcctgaatat acggttcatc
     5641 cgatatcaag ggggactctg tcgcataaag aggcgtcacc gtgtgaccca ctcgcttcac
     5701 gatcttgtac ccatctccag tagaaccagt agatggataa gtccgtccac cagttgttaa
     5761 gataatggca ggagcatgga actcttcgaa ttccgtgcgt ataccgtaaa ttttatgttc
     5821 tttgtggatc aatttctcta ctcgagtaga gaatagaagc gtcacatcta gttctttcag
     5881 acgattgagc aatgcttcga taatcgtttt tgatttattt gttacaggaa acatccgtcc
     5941 atgatcctct tcttttaaat gtacgccttg tgattcaaag aagttcatga tatcgaaatt
     6001 attccattgc gcaaacgtac tatataaaaa ctttccattt ccaggaatat gtgcaatcaa
     6061 atcatcaaca ggacgattgt tcgtaacatt gcaccgacca ccacctgtca tcagtagttt
     6121 ttttcctgct tttttgtttt tttcgatcaa gagtacgcgt gcgccttgtt cagctgcact
     6181 gatcgctgcc atcatcccgc ttgttccagc gccgactacg atcacatcat acagtttttc
     6241 cattccctca cctcaaggaa agtgtagcat atctgaagaa agaaaacaga aaaaaatgtt
     6301 ttgagccaaa agagaaaatg agctaaaatg agggaagata agcaaaggag cgtggaacga
     6361 tgacaaacgc aacagaatat gttcaagcaa ttcaagaaaa gctacatcaa aaggatcaag
     6421 ggcaagaaga atttttacaa gcaatcgatg aattcatgcc cacggttatg tcttttttgg
     6481 acactcatcc cgaatatatc gaaaagaata tcttagaact tttaacggaa ccagaaagag
     6541 ttattcaatt tcgagtgcct tggcaagatg atgaaggaaa ctggcgagta aatagagggt
     6601 accgtatcca atacaactca gctatcggcc cttataaagg tggattgcgc ttccatccta
     6661 gtgtcaatct aagtatcttg aaatttttgg cttttgaaca gattttcaag aatagtttaa
     6721 ccggtctgcc aatcggtgga ggaaaaggcg gaagtgactt cgatcctaaa ggaaaatctg
     6781 ataatgagat catgcgtttt tgtcaaagtt tcatgctgga attgtcaaaa catatcggcc
     6841 catcgattga tgttcctgcg ggagatatcg gtgtaggagc tagagaaatc ggctatttgt
     6901 tcggcgaata taaacgattg aaaaaatatg atactggtgt gttgacaggt aaacctttgg
     6961 atttttgggg aagtaagata cgtacggaag ctactggtta tggattagtg tattatgtca
     7021 aacatctatt gaacgaagaa aaagattcat ttaatcaaaa aacagttttt gtttccggaa
     7081 gcgggaatgt agccatttat gcaatcgaaa aagtgcaaga gctaggcggg aaagttgtta
     7141 catgttcaga ttctagcggg tacatctatg atccagaagg aatagatgtg aaactattga
     7201 aagaagtcaa agaaatcaat agacaacgtt tgacggaata cgcaaaccaa cgtccttctg
     7261 ctatttatca tgctggagaa tctgtttgga cattgaaaga aaaagcggat atcgctttgc
     7321 catgtgctac tcaaaatgaa atagacgaaa gcttggccga aatcttagta gaaaatggga
     7381 tcaaaatcgt agcagaaggc gctaatatgc catgtacagt aaaagctgtt tctgttcttc
     7441 atgaagcagg cgtgtggtat tgtccaggaa aagctgctaa tgctggagga gtagcagtat
     7501 ctgctttaga aatgagtcag aacgtccagc gattgacttg ggaaagagag caagtggatg
     7561 agcagctgga tcaaatcatg gaaacgattt accatacgtg tcgccaaaca gcagccgagt
     7621 acggcgacga aaaaaatctg cttcttggag caaatgttgc tggatttgaa aaagttgcca
     7681 aagcaatggc agcagaaggt ttagtttgaa aaatgaaact ttcttggaaa tattgtcagc
     7741 attttgttta tttgcttgaa aaataataaa aaaaaacgta aagtattacc tgtagagtaa
     7801 acctataagg aggagaacaa atggcacaca ttcattttga ctattcaaaa gtagcaccgt
     7861 ttgtcaacga acaagagtta gggtacatgc aaagccaagt aacagcagcc cacaacgaat
     7921 tacgggaagg tactggcgca ggaagcgact tccgcggctg gatcgattta ccaacgaact
     7981 atgacaaaga agaatttgca cgtatcaaag aagcagctaa aaaaatccaa tctgattctg
     8041 aagtattagt tgttatcggt atcggtggtt cttatttagg cgctcgtgca gcgatcgact
     8101 tcttgaatca cacattcttc aatttattag atagcaacac aagaaaagca ccacaaatct
     8161 tctttgccgg aaattcaatc agctcaacat atattgctga cttgatcgaa gtaatcggtg
     8221 atcgcgactt ttcagtaaac gttatttcaa aatctggaac aactactgaa ccagcgattg
     8281 cgttccgtgt attcaaagag cttcttgtta agaaatatgg acaagaagaa gcaaacaaac
     8341 gtatctacgc tacaacagat aaagcaaaag gtgcagttaa agtagaagca gatgcagaag
     8401 gttgggaaac tttcgtgatt cctgatgaca tcggcggacg tttctctgta ttaactgctg
     8461 ttggtttgtt accaatcgct gttagtggtg cagatatcga tgcattgatg caaggtgccg
     8521 ctgatgcaag caaagcatac tcaagcgaca aactagaaga aaacgaagct taccaatacg
     8581 cagcaatgcg caacattctt taccgtaaag gcaaagtaac tgagttgttg atcaactatg
     8641 aaccaggaat gcaatacttc tcagaatggt ggaaacaatt gtatggtgaa tctgaaggaa
     8701 aagaccaaaa aggcatctat ccttcaagcg caaacttctc aactgatttg cactctttag
     8761 gacaattcat ccaagaagga cgtcgcaata tttttgaaac agttgtgaag gttgaaaaac
     8821 ctcgtaaatc aatcacaatt cctgaacaag cagaagattt agacggtcta ggctacttgc
     8881 aaggaaaaga agtggacttc gtaaatacaa aagcatttga aggaacattg cttgctcata
     8941 cagacggtga cgtgcctaac ttgctagtca aaattccaga aatggatgct tatacattgg
     9001 gttatacaat gtacttcttt gaaattgctg taggtatttc aggttacttg aatggaatca
     9061 acccattcga ccaaccaggt gtagaagctt acaagaaaaa catgttcgca cttctaggca
     9121 aaccaggctt tgaagatctt gctaaagaat tgaacgaacg cctgtaagat aagggttttt
     9181 cgctagattt aaataatcag tgatagaaac atgttcgctt taaaatgaaa actggtaccc
     9241 atttaggtac tcaaacatac tgcatcgttt attcagaata aggggctgtg acaagacgtt
     9301 tgtcacagtc ccttgtttgt atagaaatcg attgattgcc ttgctcttta gtttgtttta
     9361 ggactttaag aacagtgagg ctgacttcga aggttaattc tgtttttttg ttttctagaa
     9421 tcagtaagca aaaaagcact cacttcgagt gcttttttgc gatatattct tagcttaaga
     9481 taggcctaat ttttctttta cagatgaggg taccttatcg ctggtaatta cttttttctc
     9541 tatgatccgc ttttcgcttg ctttcaactg caagatcgtt ccttcggggt aagcagaacc
     9601 attggcagag tcaccagaag taatgacatc gaaatctaac tgtctttttt caccgttttc
     9661 agcataagca tctacgttgt atttaaaacc tcttccaatt acttccccat tatctgcttt
     9721 gatatcagtt tctgcaggca ttggagcttt gactaccgca taataatctt tacctacata
     9781 agtagactga tagtagtccc aacctttcca tcctataaaa gcaatcaata tgataacgat
     9841 caaccctaaa atttttttca tgaaagatct ccttttttat ttttat
//
LOCUS       DASHHB010000081         9840 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.085, whole genome shotgun sequence.
ACCESSION   DASHHB010000081 DASHHB010000000
VERSION     DASHHB010000081.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9840)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9840)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9840
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.085"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(56..415)
                     /gene="rplT"
                     /locus_tag="U2K71_002509"
     CDS             complement(56..415)
                     /gene="rplT"
                     /locus_tag="U2K71_002509"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HEM7316530.1"
                     /translation="MARVKGGTVTRKRRKRVLKLAKGYYGSKHTLFKTAKEQVMNSYN
                     YAYRDRRQKKRDFRKLWIARINAAARMNGLSYSKLMHGLKVAEIDINRKMLADLAVND
                     AAAFTALADQAKEALAK"
     gene            complement(468..668)
                     /gene="rpmI"
                     /locus_tag="U2K71_002510"
     CDS             complement(468..668)
                     /gene="rpmI"
                     /locus_tag="U2K71_002510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001125942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HEM7316531.1"
                     /translation="MPKQKTHRGSAKRFKRTGKGGLKRFRAFTSHRFHGKTKKQRRQL
                     RKASMVSSGDFKRIRQQLAKMK"
     gene            complement(740..1264)
                     /gene="infC"
                     /locus_tag="U2K71_002511"
     CDS             complement(740..1264)
                     /gene="infC"
                     /locus_tag="U2K71_002511"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HEM7316532.1"
                     /translation="MTIAKDMMVNDGIRARELRLIGSDGEQLGVKTKAEALNIAEQAN
                     LDLVLVAPGAKPPVARIMDYGKYRFEQQKKDREARKKQKVINVKEVRLSPTIDLNDFN
                     TKLRNARKFLEKGDKVKASIRFKGRAITHKEIGQKVLNRLAEETADIATVEQKAKMDG
                     RSMFLTLAPKNDSK"
     misc_feature    complement(1308..1433)
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00558"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="ribosomal protein L20 leader region; Derived by
                     automated computational analysis using gene prediction
                     method: cmsearch."
                     /db_xref="RFAM:RF00558"
     gene            1584..2216
                     /locus_tag="U2K71_002512"
     CDS             1584..2216
                     /locus_tag="U2K71_002512"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HEM7316533.1"
                     /translation="MKKIEPIPPSLPLLTKGEFYLEDEEIFSGLLINGGNQSYLDAKN
                     MVLRESHIQKLTMQKTGLRHFECSNVIFEKCDFSNLEWIGGSFHQVVFHQCKLTGTNF
                     AESYLRDCTFTDCIASMASFSSTNLKAVSFDHCQLEDSEFYEVTWKNLFLSDNQLTGS
                     NWFRTSLKGLDFTTNFFTTIALSQDSLAGLIVNQEQALVIAQALGLQIKD"
     gene            complement(2218..3123)
                     /locus_tag="U2K71_002513"
     CDS             complement(2218..3123)
                     /locus_tag="U2K71_002513"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium transporter CorA family protein"
                     /protein_id="HEM7316534.1"
                     /translation="MREKHYFNQDRNFWINVQQNDVLAVKNLKEEYGISDEMLTYSLD
                     KNERARVEYDSFDEALLLVSNVPHQQKVDNHYETSPIAFILKEDGLFTFTTPNTEYVI
                     RLIRSLLERMPDMSVYSLLFRTLFLISDSFFPLIEEVNSERQRLNLKLREKTTNKNLL
                     QLSDLEIGLVYLVTGTKQNVVLLEQIKALAIYRKLSEKEKEELDDALIEARQAVEMTN
                     LASQILDQLSGTYNNLLNNNLNDTMKFLTVWSLILTVPTIVTGFFGMNLQLPFTHSVF
                     GWEIALIISLVLSIWMLIALWRRIR"
     gene            complement(3208..3867)
                     /locus_tag="U2K71_002514"
     CDS             complement(3208..3867)
                     /locus_tag="U2K71_002514"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002356607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HEM7316535.1"
                     /translation="MSVIVLAGTIGAGKSSLTEMMAEHFDSQAFYESIDDNEVLPLFY
                     ANPEQYAFLLQIYFLNKRFASIKQAMKDDNNVLDRSIYEDSLLFHLNADLGRATETEV
                     CVYDELLENMMEELPYAAHKKHPDLLVHIRVSFDTMLERIEKRGRSYEQLSFDPSLYD
                     YYKELNRRYDQWYEEYKESPKIQIDGDRYNFVEDPQAKEEVLKMIEEKLAEIRQTKVS
                     I"
     gene            4183..5127
                     /gene="mvk"
                     /locus_tag="U2K71_002515"
     CDS             4183..5127
                     /gene="mvk"
                     /locus_tag="U2K71_002515"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HEM7316536.1"
                     /translation="MANYGQGESSGKIILMGEHAVVYGEPAIAFPFYATKVTAFLEEL
                     DAMDDQLVSSYYSGNLAEAPHALKNIKKLFIHLKKQHDIQKNLQLTIESTIPAERGMG
                     SSAAVATAVTRAFYDYLAFPLSREILLENVQLSEKIAHGNPSGIDAAATSSLQPIYFT
                     KGHPFDYFSLNIDAFLIVADTGIKGQTREAVKDVAHLFETQPHETGQMIQKLGYLTKQ
                     AKQAIIENSPETLAQTMDESQSLLEKLTISNDFLNLLIQTAKDTGALGAKLTGGGRGG
                     CMIALAQTKTKAQEISQALEDAGAAETWIQGLGVHTYV"
     gene            5120..6097
                     /gene="mvaD"
                     /locus_tag="U2K71_002516"
     CDS             5120..6097
                     /gene="mvaD"
                     /locus_tag="U2K71_002516"
                     /EC_number="4.1.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphomevalonate decarboxylase"
                     /protein_id="HEM7316537.1"
                     /translation="MFKGKARAYTNIALIKYWGKKNEELILPMNNSLSLTLDAFYTET
                     EVIFSDSYMVDEFYLDGTLQDEKATKKVSQFLDLFRKEAGLSLKASVISQNFVPTAAG
                     LASSASGLAALAGACNTALKLGLDDLSLSRFARRGSGSACRSIFGGFVEWEKGHDDLS
                     SYAKPVPSDSFEDDLAMVFVLINDQKKEVSSRNGMRRTVETSSFYQGWLDSVEGDLYQ
                     LKQAIKTKDFQLLGETMERNGLKMHGTTLAAQPPFTYWSPDSLKAMDAVRQLRKKGIP
                     CYFTMDAGPNVKVLVENSHLSEVQETFTKLFSKEQVITAHAGPGIAIIE"
     gene            6110..7195
                     /locus_tag="U2K71_002517"
     CDS             6110..7195
                     /locus_tag="U2K71_002517"
                     /EC_number="2.7.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomevalonate kinase"
                     /protein_id="HEM7316538.1"
                     /translation="MIEVSAPGKLYIAGEYAVVETGHPAVIAAVDQFVTVTVESARKV
                     GSIQSAQYSGMPVRWTRRNGELVLDIRENPFHYILAAIRLTEKYAQEKNILLSFYDLK
                     VTSELDSSNGRKYGLGSSGAVTVATVKALNVFYALNLSQLEIFKIAALANLAVQDNGS
                     CGDIAASCYGGWIAFSTFDHPWLQEQETQHSISELLALDWPGLSIEPLIAPEDLRLLI
                     GWTGSPASTSDLVDQVHRSREDKMVAYQLFLKNSTECVNEMIKGFKENNVTLIQQMIR
                     KNRQLLHDLSAITGVVIETPALNKLCNLAEQYEGAAKSSGAGGGDCGIVIVDQKSGIL
                     PLMSAWEKAEITPLPLHVYSDQRKENR"
     gene            7192..8256
                     /locus_tag="U2K71_002518"
     CDS             7192..8256
                     /locus_tag="U2K71_002518"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HEM7316539.1"
                     /translation="MNRKDEHVSLAKAFHDKQKNEFDFVRIIHNPLPQIAVSDVDLST
                     QAVGFTLSSPFYINAMTGGSEKTKKINQDLAIVAREADLMIATGSVSAALKDPSLADT
                     YTIMRQEYPHGKIIANIGAGTSVERAQEAIRLFHADALQIHLNAPQELVMPEGDRDFT
                     NWKVLIQETQTAIDVPLIVKEVGFGMTRETLNDLAALGVHTVDISGRSGTSFTQIENA
                     RRSKRELSYLADWGQSTVSSLLEANEADTSMEILASGGIRNAYDIFKALCLGANAVGT
                     SGTVLTHLMNHGVEETIILMKQWQEELRLLYTMVGATNTAALHQQSLIFSGPVKDWCE
                     ARGIDLVKYGRRTEKSVGQK"
     gene            complement(8505..9308)
                     /locus_tag="U2K71_002519"
     CDS             complement(8505..9308)
                     /locus_tag="U2K71_002519"
                     /EC_number="1.1.1.69"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010741490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 5-dehydrogenase"
                     /protein_id="HEM7316540.1"
                     /translation="MEFSMDMFRLDGKVALVTGAVYGIGFEIARSLATAGAKIVFNNL
                     NQESVDEGIAHYKEAGIDAKGYVCDVTDEEAVQAMVQQIKEDVGSVDILVNNAGIIKR
                     TPMIEMDAADFRQVIDVDLNAPFIVSKAVIRDMIEKGGGKIINICSMMSELGRETVSA
                     YAAAKGGLKMLTKNIASEYGQYNIQCNGIGPGYIATPQTAPLRETQENGERHPFDQFI
                     VGRTPAARWGEPIDLAGPSVFLASSASDFVNGHVLYVDGGILAYIGKQP"
     gene            complement(9328..9633)
                     /gene="kduI"
                     /locus_tag="U2K71_002520"
                     /pseudo
     CDS             complement(9328..9633)
                     /gene="kduI"
                     /locus_tag="U2K71_002520"
                     /inference="COORDINATES: protein motif:HMM:NF002091.0"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="5-dehydro-4-deoxy-D-glucuronate isomerase"
     gene            complement(9615..>9840)
                     /locus_tag="U2K71_002521"
     CDS             complement(9615..>9840)
                     /locus_tag="U2K71_002521"
                     /inference="COORDINATES: protein motif:HMM:NF018513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TRAP transporter large permease subunit"
                     /protein_id="HEM7316541.1"
                     /translation="LWALGIMLVAFIVAKKNKYPVVEKGQMAHAGKIIKEAIPSLLLI
                     VIIIGGILTGIFTAVEASAIAVIYKWVIRF"
BASE COUNT         2892 a         2071 c         1904 g         2973 t
ORIGIN      
        1 aagaggaaag aacataactt ttcgttatct tctttcctct ttataattgt ttagcttatt
       61 tagccaaagc ttcttttgct tggtcagcca atgcagtaaa tgctgctgca tcgttaacag
      121 ccaagtcagc taacatttta cggttgatgt cgatttcagc aactttcaaa ccgtgcatta
      181 attttgagta gctcaagcca ttcatacgag ctgctgcgtt gatacgagca atccacaatt
      241 tacggaagtc gcgtttcttt tgacgacgat cgcgatatgc gtagttgtat gaattcatta
      301 cttgttcttt tgctgtttta aataaagtgt gttttgaacc gtagtaacct tttgctaatt
      361 ttagcactct tttacgacgt ttacgagtta ctgttccacc tttaacacgt gccatgtata
      421 attcctccta ataaaaataa atcttcagat tctgttattt gtacttctta tttcattttt
      481 gctagttgtt ggcgaatacg tttgaagtcg ccactagaaa ccatgcttgc tttacgcaat
      541 tgacggcgtt gtttttttgt tttgccgtgg aaacggtgac ttgtaaacgc gcggaagcgt
      601 ttcaatccgc ctttaccagt acgtttgaaa cgttttgctg atccgcggtg tgttttttgt
      661 tttggcatga ctatatttcc tcctcaaaat ctttcgttat ccgactatcg gctgtccaac
      721 agccagttag tcattcaaat tacttactgt cgtttttcgg tgccagcgtc aagaacatgc
      781 tgcgtccgtc cattttcgct ttttgttcca ctgttgcaat atccgcagtt tcttcagcta
      841 agcgattcaa gactttttga ccaatttctt tatgggtaat ggcacggcct ttgaaacgga
      901 tagaagcttt cactttatcg cccttttcca agaacttacg tgcattacga agttttgtat
      961 tgaagtcatt caagtcgatc gtaggactta agcgaacttc ttttacattg atcacttttt
     1021 gttttttacg cgcttcgcgg tctttctttt gttgttcgaa acggtattta ccgtagtcca
     1081 tgattcgcgc aactggtggt ttcgcaccag gggcaactaa cacaagatcc aagtttgctt
     1141 gttctgcaat attcaatgct tctgcttttg tcttaactcc taattgctca ccatctgaac
     1201 cgatcaatcg taactctcgt gcacgaatgc cgtcgttaac catcatatcc tttgctatgg
     1261 tcattcacct ccaaatatta tgagagaaag aaaagcttac cgaataaacg gcaataaaaa
     1321 aacgagttct cttacagaac ccgcgcgtac ttcacctaat agaaccagtg aaaactatct
     1381 agcccagtga cattatcaac taaggcgaga agcgggtgct tctgctttta tttctcaacc
     1441 attatagtat agcaaaaact aatctttttg tcaatgtttt tcatacagtt tgtcttcctt
     1501 ttctgaaaat gattagatta gcaggctttc tagcagtgat tcccgcttct ttgctatact
     1561 aaagaaaaac gaggtgaacc cacatgaaaa aaatcgaacc tattccacct tctcttcctc
     1621 ttcttacaaa gggagagttt tatttagaag atgaggagat cttctctggt cttctgatca
     1681 acggaggtaa ccaaagctat ctcgatgcca aaaatatggt attaagagag agtcatatcc
     1741 aaaaacttac gatgcaaaaa acaggtttac gtcattttga atgcagtaat gtcatttttg
     1801 aaaaatgtga tttttccaat ctggagtgga ttggtggcag tttccaccaa gtcgtgtttc
     1861 atcaatgtaa attaacaggt acgaattttg ccgaaagtta tcttcgagac tgtactttca
     1921 ctgattgtat tgcttcgatg gcttccttta gtagtacaaa cttgaaagct gtctccttcg
     1981 atcattgtca attagaagac agcgaatttt atgaggtcac ttggaaaaac ctttttttgt
     2041 ctgacaatca gttaaccggt tcgaactggt ttcgaacttc tctgaaggga ttagacttta
     2101 cgactaattt cttcacaaca atcgctttat ctcaagattc tttggcggga ctaatcgtca
     2161 atcaagagca agcacttgtg atcgcccaag cccttggttt gcagatcaaa gactagatca
     2221 acgaatccgc cgccacagtg caataagcat ccaaatagat aagaccaaac tgatgatcaa
     2281 ggcgatttcc catccgaaaa cggaatgggt aaagggtaac tggagattca ttccgaagaa
     2341 tcctgtcaca atagttggaa cggtcaagat cagcgaccag accgtcaaaa atttcatcgt
     2401 gtcattcaag ttgttattca gcaaattatt ataagtgcca gagagctggt ctaaaatctg
     2461 cgaagctaag ttcgtcattt caactgcttg tcgtgcttcg atcagtgcat cgtccaactc
     2521 ttctttttcc ttttctgaca acttgcgata aatagccagt gccttgattt gttctaaaag
     2581 tactacattt tgttttgttc ctgtaactaa gtaaaccaaa ccgatctcta aatctgaaag
     2641 ttgcagcaga ttcttgttcg ttgttttttc tcttaatttc agattaagcc gctgacgttc
     2701 actattcact tcctcgatta aaggaaaaaa ggaatcagaa atcaaaaaca atgtacgaaa
     2761 caataaactg tatacactca tgtctggcat ccgttccaaa agcgatcgaa ttagtcgaat
     2821 cacgtactcg gtattcggcg tggtaaatgt aaacaaaccg tcttctttta aaataaaagc
     2881 gataggacta gtttcataat gattatcgac tttttgctgg tgcggaacat ttgatacaag
     2941 cagtaacgct tcatcaaaag aatcgtactc tacgcgcgcc cgttcatttt tatctagaga
     3001 ataagtcagc atctcgtcac taatgccata ttcttctttt aagtttttaa cagctagcac
     3061 atcgttttgc tgtacattga tccaaaaatt acggtcttga ttaaaatagt gtttttcccg
     3121 catactctat cctcctgaca tttgtttcca gtataaagaa aaaagtcgct caaataaagg
     3181 tttacccttt atttgagcaa ctgtaaatca aatggatact ttggtttggc gaatctctgc
     3241 taatttttct tcgatcattt tcaagacttc ttcttttgct tgaggatctt ccacaaaatt
     3301 atagcggtct ccatcgattt ggatttttgg actttctttg tattcttcat accattgatc
     3361 ataacgtctg ttcaattctt tgtaataatc gtatagactt gggtcaaagg acaattgttc
     3421 atacgaacga ccacgttttt cgatacgctc taacatcgta tcaaaagata cacgaatatg
     3481 aactaataaa tccggatgtt ttttatgggc agcataggga agttcttcca tcatgttttc
     3541 cagcagttca tcatacacac aaacttctgt ttctgttgct cttcctagat ctgcatttag
     3601 atggaaaagc agtgaatctt cataaatcga acggtccaat acattattgt catctttcat
     3661 tgcttgtttg atgcttgcaa aacgtttatt caaaaaataa atttgcagca aaaaagcata
     3721 ctgttcaggg tttgcataaa ataagggcaa gacttcatta tcatcaatcg attcataaaa
     3781 tgcctgacta tcaaagtgtt cagccatcat ttctgttaaa cttgattttc cggcaccaat
     3841 tgtacctgct aaaacgatca cgctcattta cctccagctc acttcatcac taaatattgt
     3901 gtttaactcc aacctttttt aaatataccc ccaatatcta gtagtgtcaa ttgtattttt
     3961 tctagtcaaa gaagaaaaaa agtcaaaaaa ttcttaagaa aaaagtgttt tttctttaga
     4021 aaacgtattc ttagttttac ttctgagatg gttttagtaa aataaaggag gaatgttttt
     4081 tgagaccctt agtggacaaa aaaattcgtg aagcagcgca actcgttttg ggcgctttat
     4141 tttttgctta aaaatcactt ttacgtgaga aggagttaga ttatggcaaa ctatggtcaa
     4201 ggagaatcaa gcggaaagat catattgatg ggcgagcacg cggttgttta tggagaacca
     4261 gcgattgcct ttcctttcta tgcaacaaaa gtcaccgcat tccttgaaga gctggatgca
     4321 atggacgatc aactggtttc ttcctactat tcaggaaatt tagccgaagc tcctcatgca
     4381 ttaaaaaata tcaaaaaatt attcattcac ttaaaaaaac agcatgacat ccaaaaaaac
     4441 ttgcaactga ccattgaaag cacgattcct gctgaacgtg gaatgggatc aagcgctgca
     4501 gtcgccacag cagtcactcg tgctttttat gattacttag catttccttt gtctcgtgaa
     4561 atactattag aaaatgtcca gctttcggaa aaaatcgccc acggtaatcc tagtggaatc
     4621 gatgcagccg ctactagcag cttgcagccg atttatttta caaaagggca tccttttgac
     4681 tacttttctt tgaacatcga tgcttttttg attgtcgctg atacaggaat caaaggacaa
     4741 acaagagaag ccgtcaaaga tgtcgctcac ctctttgaaa cccagcctca tgaaactgga
     4801 caaatgatcc aaaaattagg atacttgacg aagcaagcaa aacaagcaat cattgagaat
     4861 tcaccagaaa cgttggcaca gacaatggat gaatcacagt cacttctgga aaagctgaca
     4921 atcagcaatg attttcttaa tctattgatc caaacagcaa aagataccgg ggcattgggt
     4981 gccaaattaa ctggcggtgg acgcggtggc tgtatgattg cattagcaca aacaaaaaca
     5041 aaagcccaag aaatcagtca agcacttgaa gatgcgggtg ctgctgaaac ttggatacaa
     5101 ggattaggag tacataccta tgtttaaagg caaagcacgc gcatatacca acattgctct
     5161 aatcaaatat tggggtaaga aaaatgaaga actcatcctt ccaatgaaca acagcctttc
     5221 gttaacattg gatgcatttt atacagagac agaagtcatc ttttctgata gctatatggt
     5281 agatgaattt tatctagatg gcaccttaca agacgaaaaa gcaacaaaaa aagtcagtca
     5341 gtttcttgac ctttttcgta aagaagccgg cctctcacta aaagcttcag taatcagtca
     5401 gaatttcgtt cctaccgcag ctggattagc ctcctctgcc agcgggctag ctgctttagc
     5461 aggagcttgc aatactgctc ttaagcttgg attagacgat ctctctcttt caagatttgc
     5521 tcgacgcggg tctggttcag cttgccgaag tattttcggt ggtttcgttg aatgggaaaa
     5581 aggccatgac gacttaagtt cttacgctaa gccagtccct tccgattctt tcgaagacga
     5641 tttagcaatg gttttcgttt tgatcaacga ccagaaaaaa gaagtgtcca gcagaaatgg
     5701 gatgcgtcga acagtcgaaa catccagttt ttatcaaggc tggttagatt ccgttgaagg
     5761 ggatctatat caattgaaac aagcaatcaa aacaaaagat ttccaacttc tcggagaaac
     5821 gatggaaaga aacggactaa aaatgcatgg aacgacttta gctgcccagc ctccattcac
     5881 ttactggtct cctgacagct taaaagcgat ggatgcggtg cggcaattga gaaaaaaagg
     5941 cattccatgc tactttacga tggatgctgg ccctaatgtc aaagttttag tggaaaacag
     6001 ccatttatct gaggtacaag aaacatttac caaattattt agcaaagaac aagtaattac
     6061 tgcccatgct ggtccaggaa ttgcgattat tgaataaagg gagattttta tgattgaagt
     6121 atctgcacca ggcaaactct atattgccgg agaatatgca gttgttgaaa caggacatcc
     6181 agcagttatc gctgcagtcg atcaattcgt aacagtcact gtagaatccg cacgaaaagt
     6241 cggaagtatc caatctgcac aatatagtgg gatgcctgta cgttggacaa gacgcaacgg
     6301 agaattagtt ttagatattc gggaaaatcc ttttcattat atccttgctg ctattcgttt
     6361 gactgaaaag tatgcacaag aaaaaaacat tcttttatcc ttttatgatc tgaaagtaac
     6421 aagtgagtta gacagttcaa atggccggaa atatggattg ggttctagcg gtgccgtgac
     6481 cgttgcaacg gtcaaagcct tgaatgtttt ttacgcgttg aatttatctc aattggagat
     6541 tttcaagatt gcagcactag ccaatttagc agttcaagat aatggttcct gcggcgacat
     6601 cgctgctagc tgttatggtg gctggatcgc tttctcaacc ttcgaccatc cttggctcca
     6661 agaacaagaa actcagcatt ctatcagtga gttacttgcc ctggattggc caggtctatc
     6721 cattgagcca ttgattgctc ctgaagattt acgtttattg attggttgga cgggtagccc
     6781 tgcctctact tctgatttgg tcgatcaagt tcaccgttcg agagaagaca aaatggtggc
     6841 ttatcagctt ttcttaaaaa acagtacaga atgtgtcaac gaaatgatca aagggtttaa
     6901 agaaaataat gtaacgttga tccaacagat gattcgaaaa aaccgacaat tactgcatga
     6961 tttatctgca atcactgggg tcgtcatcga aacgcctgct ttgaacaaat tgtgtaattt
     7021 agctgaacag tatgaaggag ccgcaaaatc ttctggtgca ggtggtggcg attgcggaat
     7081 cgtaattgtt gaccagaaat ctggcatcct tcctttaatg agtgcatggg aaaaagcaga
     7141 aatcactcca ctgccgttac atgtctatag cgatcaaaga aaggaaaacc gatgaatcga
     7201 aaagacgaac atgtttcact agctaaagcc tttcatgata aacaaaaaaa cgaatttgac
     7261 ttcgtacgga tcatccataa ccctttgccg caaatagcag tttctgatgt cgatctaagt
     7321 acacaggcag ttgggtttac actaagcagc ccattttata tcaatgcgat gactggcgga
     7381 agcgaaaaaa caaaaaaaat caaccaagat cttgcaatcg tcgcaagaga agcagatttg
     7441 atgattgcca ctggttcagt cagcgctgcc ttgaaagacc cttctttagc cgatacttat
     7501 acgattatgc gacaagaata tcctcacggg aaaatcattg ccaacatcgg tgcagggact
     7561 tctgtcgaaa gagcccaaga agctatccga ttgtttcacg cagatgcctt acagattcat
     7621 ttgaatgcac ctcaagagtt ggttatgcct gagggagacc gtgattttac caattggaaa
     7681 gtacttattc aagagactca aacagccatt gacgtccccc tcatcgtaaa agaagtcggt
     7741 tttggcatga caagagaaac gctcaatgat ctagctgctt taggagttca tacagttgat
     7801 atcagcggtc gaagcggaac aagcttcacc cagatcgaaa acgcccgtcg ctccaagcgg
     7861 gaactgagtt atttagctga ctgggggcaa tcgacagtct cttcattgct tgaagcaaat
     7921 gaagcagaca cttctatgga aatcctagcc tccggaggta tccgcaatgc ctatgacata
     7981 tttaaagccc tttgtctcgg cgcaaatgca gtgggtactt caggaaccgt actgacgcat
     8041 ctgatgaatc acggcgtaga agaaactatt atactgatga agcaatggca agaagagctt
     8101 cgtctgcttt atactatggt cggcgcaacg aataccgcgg ccctacacca acaatcactg
     8161 atcttttccg gaccagtgaa ggattggtgc gaagcaagag ggatcgactt agtgaagtat
     8221 ggaagacgca cagaaaagag cgtgggacaa aagtaaagaa cacttttatc ccacgctcta
     8281 aacacgtata aacggcggaa gcaaaagcaa ctccttcgga aataagctga aattcacaaa
     8341 aatttgaaaa gcaattttcg tgaattcctt cttatttctt ggagttaaac gcttctgtcc
     8401 cagcctcttt tttcgaataa atgatgttta atcagctgtc cttcggtatt cgttcaagcc
     8461 gctgatttca caacctctcc actaaacggt gtactaaagc tcttttaagg ttgttttccg
     8521 atataggcaa gtatgcctcc gtccacatac aagacatggc cgttaacgaa atcagatgcg
     8581 ctagaagcca agaagacaga tggtccagcc aaatcgattg gctcgcccca gcgtgctgct
     8641 ggcgtacgtc caacgataaa ttgatcgaat ggatgacgtt caccgttttc ttgtgtttcg
     8701 cgtaatggag cagtttgcgg tgtagcaata taacctggtc caattccatt acattggata
     8761 ttgtattgac cgtattcaga agcgatgttt ttcgtcagca tttttaatcc gccttttgcc
     8821 gcagcataag cactgaccgt ttcgcgtcct aattcactca tcatggagca gatattgatg
     8881 attttaccgc cgcctttttc gatcatatcc cgaataactg cttttgaaac gatgaatggg
     8941 gcatttaaat ccacgtcgat tacttggcgg aagtctgccg catccatttc gatcatcggt
     9001 gtccgtttga tgatccctgc attgttgact agaatatcaa ctgaacctac atcttctttg
     9061 atctgttgaa ccatcgcttg aaccgcttct tcatcggtca catcgcagac ataacctttt
     9121 gcatcgatgc ctgcttcttt ataatgcgct ataccttcat caactgattc ttgattcaag
     9181 ttattaaata cgatttttgc tcctgctgta gctaaagagc gtgcaatctc aaatccaata
     9241 ccatagacag caccggtgac taatgctact tttccatcta aacgaaacat atccatacta
     9301 aattccatca ttttctctcc cttatgctta ttttaattga tccatttgca ccatatccat
     9361 gtctgtatag gtgatatttt ctccgcacat tgcccagatg aatgagtaat tgcttgtacc
     9421 tacacctgaa tggatcgacc agcttggcga aataatggct tgttcattgt ctacgactaa
     9481 atgttttgtt tcatctggtt tgcccatcat atggaacaca cgtgtatctt cattggcata
     9541 atcaaaatag acataagctt ccatacgacg ttcatgtgta tgacaaggca tcgtattcca
     9601 agcacttcct ggttctaaaa tcgtataacc catttataaa tgacagctat cgctgaagct
     9661 tctactgctg taaatatccc cgtcaagata ccaccgataa tgatgacaat caatagaagg
     9721 ctaggaatcg cctcttttat tatttttcca gcatgagcca tctgtccttt ttcgacaaca
     9781 gggtatttat tctttttagc aacgatgaat gctactagca tgattcctag cgcccacaaa
//
LOCUS       DASHHB010000082         9769 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.086, whole genome shotgun sequence.
ACCESSION   DASHHB010000082 DASHHB010000000
VERSION     DASHHB010000082.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9769)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9769)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9769
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.086"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(378..1834)
                     /locus_tag="U2K71_002522"
                     /pseudo
     CDS             complement(378..1834)
                     /locus_tag="U2K71_002522"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350440.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol transporter subunit IIA"
     gene            complement(1856..2158)
                     /locus_tag="U2K71_002523"
     CDS             complement(1856..2158)
                     /locus_tag="U2K71_002523"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003063077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIB"
                     /protein_id="HEM7316542.1"
                     /translation="MIKILAACGAGVNSSHQIKSALETELKNRGYQVTCDAVMIKDIN
                     EEMLSHYDIFAQIANTDLGFDINIPVVDAGAILYRIPAMAEPVYQSMESAIQSLNK"
     gene            complement(2215..2694)
                     /locus_tag="U2K71_002524"
     CDS             complement(2215..2694)
                     /locus_tag="U2K71_002524"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HEM7316543.1"
                     /translation="MNEFFFKDTVYVSDKQSQEEVFEEVYQDLLKKELVTEDFLENLL
                     ERERNYPTGLDLSPVSKILPDIAIPHTESEFVRTTRIIPIKVKNEIPFYNMISPSDQI
                     SASFLFMILNENGEAQAGMLANIMDFINSVDEEKLFSFFKLEDTEAIYHFLEEHFYI"
     gene            2859..3632
                     /locus_tag="U2K71_002525"
     CDS             2859..3632
                     /locus_tag="U2K71_002525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010731480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM7316544.1"
                     /translation="MLKTERQRSILAMCDRYGTITVKFIQEVLNVSDMTIRRDLEELA
                     QKKKLLRVHGGAQKIDYLEMSTSIEANELSHTEKKKLHTKEKEYIAKNAAAHINQEDD
                     TIFLGSGTTIELMTRYIKPSSLRIVTNSLPVFNLLSEIKRFELYLIGGLYREKTGAFV
                     GSIANEAVQKIGIKKAFVGVNGLKGSEVSNFSIEEGTLQKIVLDNAAEKCLVSDANKF
                     DHSDFYNFYDLRKIDKLFTDSTLSAELKSYYQQFTKIIQ"
     gene            3652..4080
                     /gene="lacA"
                     /locus_tag="U2K71_002526"
     CDS             3652..4080
                     /gene="lacA"
                     /locus_tag="U2K71_002526"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacA"
                     /protein_id="HEM7316545.1"
                     /translation="MRVVIGSDSDGMELKELIKKKLIKEGITVVDKSETASEDFVEST
                     LAIANDLNENEGSLGIAVDGYGAGSFITATKVKGMVAAEVSDERSAYMTRKHNNSRMI
                     TLGSKIVGDELALNIVKEFLQANYDGGRHQVRVDMLNKMA"
     gene            4099..4614
                     /gene="lacB"
                     /locus_tag="U2K71_002527"
     CDS             4099..4614
                     /gene="lacB"
                     /locus_tag="U2K71_002527"
                     /EC_number="5.3.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="galactose-6-phosphate isomerase subunit LacB"
                     /protein_id="HEM7316546.1"
                     /translation="MKIAIGCDHIVTDTKMAVSDFLKSKGHEVLDMGTYDFTRTHYPI
                     YGKKVGEAVTSGEADLGVCLCGTGVGINNAVNKVPGIRSALVRDMTTAIYAKEQLNAN
                     VIGFGARITGEFLICDILEAFINADYQETEENKKLIAKIDHVEKASDKQSDPHFFDEF
                     IEKWDRGEYHD"
     gene            4627..5553
                     /gene="lacC"
                     /locus_tag="U2K71_002528"
     CDS             4627..5553
                     /gene="lacC"
                     /locus_tag="U2K71_002528"
                     /EC_number="2.7.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015016933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-6-phosphate kinase"
                     /protein_id="HEM7316547.1"
                     /translation="MILTITMNPSIDISYPLETFKLDTVNRVRNVRKTAGGKGLNVTR
                     ILKQLDTEVIASGLIGGFLGEEIKKDLTQNEIAHDFLPISGETRNCIAVLHDGKQTEI
                     LENGPVITEQEARAFLQHFEKLAVKAEIISFSGSLPEGLPADFYRQMLQVCQPKPVVL
                     DCSGTALREVLRGSVKPKLIKPNTEELAGLLGREISTELSDLKAALSDSMFDEIEWIV
                     VSMGGDGAFAKHHETFYCVTIPKIDVVNPVGSGDATVAGLTAAIQQGLSDEAVLKQGN
                     VLGMLNAQEATTGYVQLANYQKLYDQILVEKV"
     gene            5566..6552
                     /gene="lacD"
                     /locus_tag="U2K71_002529"
     CDS             5566..6552
                     /gene="lacD"
                     /locus_tag="U2K71_002529"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEM7316548.1"
                     /translation="MNEEKRQYIKQLCNEDGVIGALAIDQRGALKKMITKFKGSEAEE
                     SEIVEFKEIVSSQLTKYASSILLDPEYGLPAADVRAKNAGLLLAYEKTGYDASTPGRL
                     PDLLSIWSVKRLKEAGADACKFLLYYDVDEAEEINNQKHAFIERIGSECQAENLPFFL
                     ELVSYDANISDATSKEYAIKKPHKVNDMMKEFSDPRYGVDVLKVEVPVNMNYVEGYGT
                     DEVVYTKAQAANFFKEQAEATNLPFIFLSAGVKAELFQETLRFAKNAGSTFNGVLCGR
                     ATWADGVESYIVDGKEAAMEWMNTQGRKNIEELNAVLKETATPVEVLTSSLS"
     gene            7008..7676
                     /locus_tag="U2K71_002530"
     CDS             7008..7676
                     /locus_tag="U2K71_002530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7316549.1"
                     /translation="MRVLVVEDNRELVLSVKKGLEQENISVDVAYDGESGEEKAFINS
                     YDGILLDLNLPKKDGLSVLQSLRAEEVESPVIIITARDEIEERAKGLDFGADDYLVKP
                     FELLELKARIRAVIRRYHGRALPQISIGALKIDPLGRRVSYQKTEVVLKPKEFDILLC
                     IAQRHPAVVSSEEISEHVYDEDFDPFSSVLRVHLARLKKKLAEAAEKEVLKTIRAKGY
                     QLCY"
     gene            7667..8800
                     /locus_tag="U2K71_002531"
     CDS             7667..8800
                     /locus_tag="U2K71_002531"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM7316550.1"
                     /translation="MLLNFKMTLTMLAFSLLVTGVILVSFFFLRNNLEGISIASTLGE
                     DVYFMEEGKTVQAETQMEASKSSNNQFMTISMIDKAYQDELFKYLPAMVLVICSAVML
                     LTFILWLVLRKIYSNQILAITRDLTFIDQVPKNTVKDEALNAAFNKLKDKFDGHLEDY
                     RRLNSYLTHEQKNAIAILRTNLEIERQHADQGNLMLLDRMTDSIDDILTLSNNSEDLS
                     TEVVDVSLICAEVCDAYKMNYPNLLFAFTEDQPTLIHGRDRWIYRAVSNLVDNAIKYG
                     EGKLVIVTISNQKGSVIIEVKDQGIGIPQEKQASIFSHQYRVHGLKQDGLPKTMDFRD
                     TDETFIQSVASKRNHIPRKSLNYRTPLEVFLSCIDKDILSSLI"
     gene            complement(9095..9454)
                     /locus_tag="U2K71_002532"
                     /pseudo
     CDS             complement(9095..9454)
                     /locus_tag="U2K71_002532"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
BASE COUNT         3143 a         1713 c         2123 g         2790 t
ORIGIN      
        1 gggatatcaa aacaaccatg gacactaagt cagctgaagc tctttgacat gaaacgagtt
       61 agatcagaaa aagcaatgat gaaatcgcac gcataaccgc gggatgcgaa gcgattcagg
      121 aaaaaagaaa tcaaggaatt cgcaattaaa caacaaaaag tcaaaaagca caaaacaaac
      181 aagaacacaa aaagaatcgt tgaggaatac caacaaaggc aagcttaaag gagttaaaaa
      241 aagcataaac gttgataatt tctttattgg gtgtggcaag agatgggcta tgctttttga
      301 aatggatttt acatctattt attttttata ggtaaaataa taccccgaag tctaaagaca
      361 ttcggggtgt ttttagctta tttagcttcg gctgcataag tcatattatg cgccgttgcc
      421 ggagatgcat tctcttcttt ttcagctgcg tatgcagcgt tgcgtttttt catttgacga
      481 gcgtaccaaa tgaagattaa aaggtatgca cctaatgcta aggcagcgta tatcacaaat
      541 tcgatttgtc cttgagaagc attaccgact aaataaccta ggaatttttc aatcgggcct
      601 tccatcgttg tatgagagat catggcggac ccgttcaagc cggctggaaa ggctccgact
      661 gctttagctg tttgagtaac gaacggggcg atcaaggtgc cggaccataa gaataatggt
      721 aattcgatcg cgccgattac gatcatacgg attaattttc cacgagttac gaccaatagt
      781 gctggggtaa cacccattgc gatgattccg cctagtggca ataatttatt tcctggtaag
      841 atgatggctt ctagcaacat gattggtgct aatacgttag cggcagccca gatttcagcg
      901 cggcctgcta tgaaaggcca gtctaaacct atgttgatgg tacgtccttt tagtcgttta
      961 ttggcaaaat cggtaatccc ttgtgataac ggttctacgg ctgcgatgaa ccaagcgcct
     1021 atcaaagaga acaattccaa acagacggca gcggtaaagg ctagcgagaa aatttgcgtt
     1081 ggtgtttgtc ctgctagtaa tccaacaaaa atccccaaat aaattccaat cgcgaatttt
     1141 gatccccaga agccaatttt actattcaat ttggcagcat cgaaatcata tttatccaac
     1201 caagggaaga ttttgtcgaa aattttatcg aaaaccataa taatcggatt catcatgtag
     1261 ttcatatgag tcgtggtcat cgggttgctg tctggcgcat ttaacaagtc attgaaggtt
     1321 ggtttcatta agtcggagtt ccaaattttt aatacaccaa tgaaaataac taatgcagaa
     1381 gcaataaaca gattagcacc gcagaagatc gcgaacaaac caacaattgc taaatgccaa
     1441 atatcgaaaa tatccacatc taatgtgttg gttttttcat tgctaacatg acaatattga
     1501 cgatcaccat aattagtaaa aaataaagcg tgtatggcga cccccatgtg atggttgcta
     1561 gtggggccca accgacatct gtaatgttta atgaaatacc tgtgcgttca acaaaagctg
     1621 tcatagcgtc tgaaaaagca gtagttaaaa ttccaataat agctccgatc ccagtcaggg
     1681 cgatccctaa tttgagcccg ccttcaagag cgcgtgacgg ttttacttta aaaaataaag
     1741 caataacagt taatacgatg gtcattaacg gtgccgcacc taaattaatt aggggggtaa
     1801 atactttatt ggcaatatca ataattgtat tcatatgctt ttcctttctt tagtcctatt
     1861 tgtttaatga ttggattgcg ctttccatac tttggtaaac aggctcagcc atagcaggaa
     1921 tacgataaag aatagcacct gcatcaacga cagggatgtt aatgtcaaaa cccaagtcgg
     1981 tgttggcaat ttgtgcgaaa atgtcataat gagacagcat ttcttcattg atatctttga
     2041 tcataacggc gtcacaggtg acttgatagc cacgattttt caattcagtt tccaaagcgc
     2101 ttttgatttg atggcttgaa ttaacgcctg ctccacatgc tgctaaaatt ttgatcataa
     2161 taattctccc cttaaaataa gtttattttt tatcctgcgg ttaggcagag acttctatat
     2221 ataaaaatgt tcttctaaaa aatgataaat cgcttctgtg tcctctaatt tgaaaaacga
     2281 aaacagtttt tcttcgtcaa cagaattaat gaaatccatg atattagcca acatgccagc
     2341 ctgtgcttca ccattttcgt tcaagatcat gaaaagaaat gaagcggaaa tctgatcgct
     2401 tggcgagatc atgttgtaga aagggatttc attttttact ttgattggaa tgatacgggt
     2461 ggttcgcaca aactcacttt cggtatgagg gatagcgata tctggcaata ttttagagac
     2521 aggtgagaga tctaatcccg tcggataatt acgctcccgt tctaaaaggt tctccaaaaa
     2581 atcttcggtt accaattctt tctttaacag atcttgatag acttcctcga acacttcttc
     2641 ctgcgactgc ttgtcagaaa catagacagt gtctttgaaa aagaattcat tcacacaaaa
     2701 cagctccttt attagttcgt ttatgtttat ttatatgttt acaagttgag tataaccgct
     2761 tacaaaatca ttgtcaacgc ataaacgaaa ataaacgaac aatatgtgtt gaaaaatgtt
     2821 cgtaaatgtt ttattataag gtcgtggagg tacagaatat gctgaaaaca gaacgacagc
     2881 gttcgatctt agcgatgtgt gatcgctatg ggacgataac agtcaaattt attcaggaag
     2941 tactgaatgt ttcagatatg acgatccgcc gagatctaga ggaactcgcg cagaagaaaa
     3001 agcttttacg cgttcatggc ggtgcacaaa aaatcgatta tctggaaatg tcgacttcta
     3061 tagaagcaaa tgagctttct catactgaaa aaaagaagct gcatacaaaa gagaaggaat
     3121 acatcgctaa aaatgcggca gctcatatca atcaagaaga cgacacgatt tttcttggat
     3181 cgggtacaac gatcgaatta atgacacgat acatcaagcc gtcatctctc cgaatcgtta
     3241 ccaatagtct gccggttttc aatctattaa gtgaaataaa aagatttgaa ctgtatttga
     3301 tcggtgggct atatcgggaa aagactggag cctttgtcgg ttcaattgcc aatgaagctg
     3361 ttcaaaaaat cggtattaag aaggcttttg tcggagtgaa tggattgaag ggttcggagg
     3421 tttcgaattt cagtattgaa gaaggtacct tgcaaaaaat tgttttagat aatgcggcag
     3481 aaaaatgttt ggtttcagat gcaaacaaat tcgaccatag cgatttttat aatttctatg
     3541 atttgcgcaa gatcgacaaa ttatttacgg attctactct ttcagccgag ctgaagagtt
     3601 attatcagca attcactaaa atcattcagt aaaaaatagg aggaaacaaa aatgcgtgta
     3661 gtaattggtt cagacagtga cggcatggaa ttaaaggaat taatcaagaa aaaattgatc
     3721 aaagaaggta ttacagtagt agataagagt gaaacggctt cagaagattt tgtggaatca
     3781 actttggcga tcgcaaatga tctgaatgaa aacgaaggta gtttaggaat cgcggttgat
     3841 ggctacggcg ctggcagctt tatcacggca acaaaggtta aaggaatggt agcagcagaa
     3901 gtttcggatg agcgcagtgc gtatatgacc cgtaaacata ataattctcg gatgatcaca
     3961 cttggatcaa aaattgttgg cgatgagttg gcgttgaata ttgttaagga atttctacaa
     4021 gcaaattatg atggtggtcg tcatcaagta cgtgtcgaca tgttaaataa aatggcgtaa
     4081 ttaggaagga tgaatgaaat gaaaatcgca attggctgtg accacatcgt cacagataca
     4141 aaaatggcag tatcagattt cttaaaatca aaaggacatg aagtgttgga tatggggacc
     4201 tatgacttta ccagaaccca ttatccgatc tacggaaaaa aagtcggaga ggccgtgact
     4261 agcggagaag cagatctagg agtttgtttg tgtggaacag gtgttgggat taacaacgct
     4321 gtcaataaag tacctggtat ccgcagtgcg ctggttcgcg atatgacgac tgctatctat
     4381 gcgaaggaac aactaaatgc caatgtgatc ggctttggcg ctcggattac gggagaattt
     4441 ttgatctgtg acatattaga ggccttcatc aatgccgatt atcaagaaac tgaagaaaac
     4501 aaaaaattga tcgccaaaat cgatcacgtc gaaaaagcca gtgacaagca gtctgatccg
     4561 catttcttcg atgaatttat cgagaaatgg gatcgtggcg aataccacga ttaagcgggt
     4621 ggacaaatga ttttaacaat aacgatgaat ccatcgattg atatttctta tccattagaa
     4681 accttcaagc tcgacacagt caatcgagta agaaatgttc gcaaaacagc tggcggtaag
     4741 ggcctaaatg tgacacgtat cttgaagcaa ttagatacgg aggtgattgc tagtggttta
     4801 atcggcggtt ttttaggtga agagattaaa aaggacctga ctcaaaatga gattgctcat
     4861 gactttttac ctatcagcgg agagacacgt aattgcatcg ctgttttaca tgacggaaaa
     4921 cagacggaaa tcctagaaaa cggacctgtt attactgaac aagaagcaag ggcgttcttg
     4981 caacatttcg aaaaattagc tgttaaagcc gaaatcattt ctttttcagg aagcttgcca
     5041 gaaggattgc ctgctgattt ttatcgacaa atgctgcaag tttgtcagcc aaagcctgtc
     5101 gttttagatt gttcaggtac agcattaagg gaagtgttgc gaggcagtgt aaagcctaag
     5161 ctaataaaac ccaataccga agagctggct ggtctgctag gacgagaaat ctcaacagaa
     5221 ttatcagatt taaaagccgc cttgtcagat tcgatgtttg atgaaattga atggatagtg
     5281 gtttcaatgg ggggcgacgg agcctttgcc aaacatcacg agacttttta ttgcgtgaca
     5341 attccaaaga ttgatgtcgt caatccagtt ggctctggtg atgcgaccgt tgccggattg
     5401 accgctgcga ttcaacaagg tctttcggat gaagccgtgt taaaacaggg taatgtttta
     5461 ggaatgctga atgctcagga agcgacgact ggctatgtcc aattagccaa ttatcaaaaa
     5521 ctatatgatc aaatcttagt ggagaaagtg taggaggata cggaaatgaa cgaagaaaaa
     5581 cgtcaatata tcaagcaact atgcaatgaa gatggagtga ttggggcctt agcaattgat
     5641 caacgcgggg ctttgaagaa gatgatcaca aaattcaagg gcagcgaagc agaagaatca
     5701 gaaatcgttg aatttaaaga aattgtttct tcacaattaa cgaaatatgc ctcatcgatt
     5761 ttattagatc ccgaatatgg cttgccagca gctgacgtta gagcaaaaaa tgccggcttg
     5821 ctgttagctt atgaaaaaac tggttacgat gcgtcaactc ccggccgttt accagattta
     5881 ttatcgatct ggtcagttaa acgtttaaaa gaagcaggcg ccgatgcttg taagttccta
     5941 ctttactatg atgtcgatga agcagaagaa atcaataatc aaaaacatgc ctttattgaa
     6001 cgaatcggct cagaatgtca ggctgaaaac ttacccttct tcttggagct tgtttcttat
     6061 gacgcgaaca tctctgatgc gacttcgaaa gaatacgcca ttaagaaacc tcataaagtc
     6121 aacgatatga tgaaggaatt ttctgatcca cgttatggcg tagatgtgtt aaaggtggaa
     6181 gtacccgtca atatgaatta tgtggaaggc tatgggacag acgaagtcgt gtacacaaag
     6241 gcacaagcgg ctaacttctt caaggaacag gctgaagcca ctaacctgcc atttattttc
     6301 ctaagcgccg gtgtaaaagc agagttgttc caagaaacac tgcgcttcgc gaaaaatgcc
     6361 ggctcaacct tcaatggtgt actttgcggc cgtgcgactt gggcagatgg tgtagaaagc
     6421 tatatcgttg atggaaaaga agcagcgatg gagtggatga atacgcaagg gcgtaaaaat
     6481 attgaagaat taaatgccgt attgaaagaa actgctacac cggtggaagt gttaacaagc
     6541 tcactatcat aagaaatcag actgtgaagc aaaattgctt gcagtctttt tttatacata
     6601 aagcgtaaaa tcatgctgca ttattagggg aacgaattct aaaatagtaa gtgagttaaa
     6661 acgaaggggt ttttctttag agagattttc ttacatgtgg tacttaatga cttgatggca
     6721 attttcagta ttctaacgag gcttattggt cataaaccaa cgaactttga aaagtatata
     6781 caagaaaacc aagcaaatag aaagtagtga gactggagga aaggttccag tctttttcta
     6841 ttctaggtat tttcaaacag taaaatcatt cttaattatg tgttttttaa cagaatgtat
     6901 gaatttcttc aaatcttcac aaaaagaacg tggactttga agaagagcgt gtactatgct
     6961 aaaaagcata agtgtaatta ctaaatccgt atgaggagtg aatatacatg cgtgtactgg
     7021 tggttgagga caatcgtgag ctggttttat ccgtaaaaaa gggcctagag caggagaata
     7081 tttctgtaga tgtagcttat gatggcgagt caggagaaga gaaggccttc atcaattcat
     7141 acgatgggat actactagat ttaaatctgc ctaaaaaaga tggattatct gttttacaaa
     7201 gtctccgtgc tgaagaagtc gaaagtccag ttatcattat tactgcacgt gatgagattg
     7261 aagaacgagc aaaaggcttg gattttggag cagatgatta tttagtcaaa ccctttgagt
     7321 tactagaatt aaaagcacga atacgcgcgg ttattcgtcg ttatcatgga cgtgcgctgc
     7381 cgcagatttc aattggtgct ttgaagattg atccattagg tcgaagggtc agctatcaaa
     7441 agacagaggt ggtcctgaaa cccaaggaat ttgatatctt actgtgcatc gctcagcgtc
     7501 atccagcggt tgtttcgtct gaggaaattt ctgaacatgt ctacgatgaa gattttgatc
     7561 ctttttcgtc ggttctgcga gtgcatctgg ctcgtctaaa aaagaaactg gctgaagcag
     7621 ctgaaaaaga ggtcttaaaa accattcgag caaaggggta ccagctatgc tattaaattt
     7681 taaaatgacg ttaacgatgc tggctttttc actgttggtg acaggcgtga ttttagtgag
     7741 tttcttcttt ctgagaaata atttggaggg aatcagtatc gcatctacct taggtgaaga
     7801 tgtttatttt atggaagagg gtaagacggt tcaagcagag acgcagatgg aagcttcgaa
     7861 atcttccaac aatcaattta tgactatttc gatgatagat aaagcttacc aggacgaact
     7921 atttaaatat ttaccagcga tggtgctggt tatttgttct gccgtcatgt tattaacgtt
     7981 tattctttgg ttggttttac gcaaaattta cagtaatcaa atcttagcga ttacgcgtga
     8041 tttaacgttt attgatcaag ttcctaaaaa tactgtgaag gacgaggcat taaatgcggc
     8101 ttttaataag ctaaaggata agttcgatgg tcatttggag gattatcgac gattaaatag
     8161 ttatctgacc cacgagcaga aaaatgccat cgctattttg cgtacgaatc tggaaataga
     8221 acggcaacat gccgatcaag gaaatttaat gcttttagat cggatgaccg acagtattga
     8281 cgatattttg acattatcca ataactcaga ggatcttagc acggaagtcg tagacgtttc
     8341 actgatctgt gcggaagttt gtgatgccta caaaatgaat tatccgaatc ttttgtttgc
     8401 gtttactgaa gatcaaccaa ccctgattca tggacgagat cgttggattt atcgggcggt
     8461 ttcgaattta gttgacaatg caatcaaata tggtgaagga aaactggtaa ttgtcaccat
     8521 ttcaaaccaa aagggtagcg tgattattga agtaaaagat cagggaattg gcattcctca
     8581 ggagaaacag gcatcaattt tttctcatca gtaccgagtg catgggctca agcaggatgg
     8641 cttacctaaa acaatggact ttagggacac ggatgaaacc tttattcaat ccgttgcctc
     8701 gaaaagaaac catattccaa gaaaatcatt aaattatcga acaccattgg aagtattttt
     8761 gagctgcata gataaagaca tattgtctag cttaatttga caaatcagat gtctataaaa
     8821 ttcgttagaa aatacaattt atagatatcc aattgatttg taaacaaaaa ggagttgtca
     8881 actaagccac tcctttttgt tgtaattttt cccaataaga aagtctacta ctttaggaag
     8941 ataatagtaa gatagaaatg ttgcccccaa aattattatc cagctggata taattaatca
     9001 aataaaaagg atcaaaaatg tagtgacccc caaaagttag acttttttgg agtggagaaa
     9061 tccactcttt ttttcatgct ctaaaatgat atttttacac aaccgtaacg gttgttttac
     9121 gaaattggac gggactttgc cagtttaatt tctgttttat ccgttcatta ttgtagtaat
     9181 agatatagct atcgatggct gattttaatt cctcgaaaca gttataaact ttcccgtgaa
     9241 aaatctcttg ttttaataag ccaaaaaaat tctccataag tgaattatct aagcagttcc
     9301 ctttccgaga catgctttga aagattttgt actccttcaa tttgtttcta taagctttca
     9361 tttgatacga ccaaccttga tctgagtgaa acgtacgacg atagggacaa tcgttagtgg
     9421 tctggatagc ttcctccaaa ccttccatta ccgcacgtgt ttctgaatgg tggtagcgac
     9481 cgatttgaac ggctagggcg gaaatacggt gcaaatgcac ggtggtttca tgcaatgctt
     9541 acagggcgta ttttgtaagg gtatgtaacg gagataatcc cgacggtaat ggatgtgaga
     9601 ggccaagccg acgctgacgg tagggcaaac aaacatagtt gcactctagc gatcgttatt
     9661 tatttttaac ggtcaaacta gggtacaact atgcttaaaa cacttcgcct caacttctgg
     9721 tagcaaaagt gattttgaaa gtcaaaatat tttttatgag ttatttata
//
LOCUS       DASHHB010000083         9229 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.087, whole genome shotgun sequence.
ACCESSION   DASHHB010000083 DASHHB010000000
VERSION     DASHHB010000083.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9229)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9229)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9229
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.087"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(58..2184)
                     /gene="pnp"
                     /locus_tag="U2K71_002533"
     CDS             complement(58..2184)
                     /gene="pnp"
                     /locus_tag="U2K71_002533"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HEM7316551.1"
                     /translation="MSEKQVFKTTWGGRPLQIEVGQLAKQANGAVLVRYGDTVVLSAA
                     VASKEAKDTDFFPLTINYEEKMYAAGKIPGGFIKREGRPSTEATLTARLIDRPIRPMF
                     AEGFRNEVQVTNIVMSVETDCSPAMAAMLGSSLALSISDIPFDGPIAGVEVGRVNGEY
                     VLNPTVEQAEQTDIELTVAGTKQAINMVESGAKEVSEEDMLGALLFGFDAIKELVAFQ
                     EEIVQAVGKEKMEVTLLQVDEVLKKEIFDASYATMKAAVMTEEKLAREDNIEQVKIDI
                     REAYAEKFAGHEDEDHLLKEVKQITEDLEKDVVRELITIDKIRPDGRKLDEIRPLSSE
                     VSLLPRVHGSGLFTRGQTQALSACTLAPLGEHQIIDGLGVEVSKRFIHHYNFPQFSVG
                     STGRAGSPGRREIGHGALGERALAQVIPSEEEFPYTIRLVAEVLESNGSSSQASICAG
                     TLALMDAGVPIKAPVAGIAMGLVSDGENYTILTDIQGLEDHLGDMDFKVAGTKDGITA
                     LQMDIKIQGITEQILTEALTQAKQARMEILEELTSTIAAPREELSQYAPKIEMIQIEP
                     AKIKDVIGKGGDTINGIIDETGVKIDIDQDGKVSIASADAEMIKKAIKIIEDLTKEVK
                     VGEVYLGKVVRIEKFGAFVNLIKGKDGLVHISQLANDRVNKVEDVVKLGDEILVKVTE
                     IDKQGRVNLSRKAMLNEDGSTKNEEK"
     gene            complement(2372..2641)
                     /gene="rpsO"
                     /locus_tag="U2K71_002534"
     CDS             complement(2372..2641)
                     /gene="rpsO"
                     /locus_tag="U2K71_002534"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HEM7316552.1"
                     /translation="MAISKERKNEIINEYARHEGDTGSPEVQIAVLTEEINHLNEHAR
                     VHKKDHHSYRGLMKKVGHRRNLLAYLRKTDVQRYRELIKRLGLRR"
     gene            2892..3455
                     /locus_tag="U2K71_002535"
     CDS             2892..3455
                     /locus_tag="U2K71_002535"
                     /EC_number="3.5.1.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HEM7316553.1"
                     /translation="MITMDDIIREGNPTLREVAKEVSLPLSEEDISLGKEMLEFLKNS
                     QDPIKAEELHLRGGVGLAAPQLDISKRIIAVHVPSPDPEADGPSLSTVMYNPKILSHS
                     VQDACLGEGEGCLSVDREVPGYVVRHAKITVSYYDMNGEKHKIRLKNYESIVVQHEID
                     HINGVMFYDHINDQNPFALKEGVLVIE"
     gene            complement(3708..4229)
                     /locus_tag="U2K71_002536"
     CDS             complement(3708..4229)
                     /locus_tag="U2K71_002536"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcription repressor NadR"
                     /protein_id="HEM7316554.1"
                     /translation="MIEGETRRKQIMQTLANTEKPVSASKFAKTFGVSRQIIVGDIAL
                     LRAAGKSIVATARGYILETEETNTGHISKIAVQHGKDQTEEELRLIVENGGEIIDVIV
                     EHPLYGELAGTLHIKTPQDIDSFMRRYQKSNATLLSELTGGIHLHTIRYPEKHMLKNI
                     KQKLAEAGILYEG"
     gene            complement(4232..5074)
                     /locus_tag="U2K71_002537"
     CDS             complement(4232..5074)
                     /locus_tag="U2K71_002537"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HEM7316555.1"
                     /translation="MTEFKTEKEKMIAGELYFANDPELVADRMFARSQSQIINQAESA
                     ELRSQLLKETFGRTGKKIYMEPVINFDYGYNIFVGENFYANFNCTFLDVSTIEIGDNC
                     MFAPNVQLYTATHPLHPVKRNSGLEYAKPIKIGNNVWLGGGVIVTPGVTLGDNVVVGA
                     GSVVTKSFPDNVVIAGNPARIIKTVEEELTEESLETLRHKIDMIDRELVALLEKRMDT
                     VTKIGQVKKEENQAVYDAKREQQVLDKVVSLLKNKGYKETITDTYVDLMKHSREYQNK
                     MKEE"
     gene            complement(5067..5792)
                     /locus_tag="U2K71_002538"
     CDS             complement(5067..5792)
                     /locus_tag="U2K71_002538"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacylase"
                     /protein_id="HEM7316556.1"
                     /translation="MKQLTVDEAAKEILAYQQITFLTGAGVSTPSGVPDYRSLSGIYH
                     GMEQPEYLLSHQAMVNEPDKFYQFVKKIYHPEAKPNIIHLEIAHLAKEKNVWVVSQNI
                     DGLHAKAKSPQLVNFHGSLYQCHCRKCQQTVDWKEYLHSDKHQACGGQIRPDIVLYGE
                     GFQDGVMEQAAYAVSQAELIVIVGTSFQVHPFCDLVQFRQPSAKILVINQTPVYLAEP
                     YTFIQEDGAVVFKKIQELGADYD"
     gene            5968..6264
                     /locus_tag="U2K71_002539"
     CDS             5968..6264
                     /locus_tag="U2K71_002539"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316557.1"
                     /translation="MLPFFIYWGVILACIAWLALSVYFSVFYLVRKENGNLWAFALFN
                     VIAAIVLAITLAVYRTWGWGITQYSSLIYLILAIYGVVVLLQAILGREPKKAAA"
     gene            6832..8424
                     /locus_tag="U2K71_002540"
     CDS             6832..8424
                     /locus_tag="U2K71_002540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327538.1"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM7316558.1"
                     /translation="MKKNMVSVAVLFAFVFSLFSVGSLANADEKTPSDEFRVGMEAGY
                     APFNWSQQTDENNALPIQGQNSYAGGYDVQIAKKVADGLGKKLVIVQTKWDGLAPALQ
                     SGKIDAIIAGMSPTAERRKEIDFTDPYYESQLVVVVQKDSKYAKAKSLADLSGAKITA
                     QLNTFHYTVIDQIPDVQKQQAMDNFSAMRTALASGMIDGYVSERPEGVTATSVNKDLE
                     MLEFSGADGFQTNPEDVQISVGMRKGDPDIQKVNQILSGISNDERTKIMDQAVKDQPA
                     ATDSEEKPGIISDFTTIWKQYGNMFLRGAGLTLFIALIGTVVGTTLGLLIGVFRSVPE
                     SGNALTRFFQKIGNALLSIYVEVFRGTPMMVQAMVIFYGLALAFGISLNRTIAALFIV
                     SLNTGAYMTEIVRGGIFAVDEGQFEAAQAIGMTHGQTMRKVVIPQVLRNILPATGNEF
                     VINIKDTAVLSVIGVADLFFQGNSASGANFQFFQTFTIVGIIYLVMTFTITRILRAVE
                     KKIDGPSAYTKLEEIDNSNLQE"
     gene            8437..9177
                     /locus_tag="U2K71_002541"
     CDS             8437..9177
                     /locus_tag="U2K71_002541"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HEM7316559.1"
                     /translation="MNEKIIEIKHLRKSFGENEVLKDISVTVNKGEVVTIIGSSGSGK
                     STFLRCINLLEKPTGGEIIYNGENVLAPKYNLPKYRTNLGMVFQSFNLFNNMNVLENC
                     MSGQTTVLKRDKETAKKVAIENLEKVGMDRYIEAKPAQLSGGQKQRVAIARALSMNPD
                     VLLFDEPTSALDPEMVGEVLNTIKDLAHTGLTMLIVTHEMEFAKDVSDRVIFMDKGVI
                     AEEGTPEDIFVHPKEERTKEFLSRILNA"
BASE COUNT         2561 a         1879 c         1831 g         2958 t
ORIGIN      
        1 aattattgtc cccctgtcac cttcacgatc acaagtacat tattcctatg aattaaatta
       61 tttttcttca tttttcgttg aaccatcttc gttcaacatt gcttttcttg acagatttac
      121 gcgtccttgt ttatcgattt cagttacttt cacaaggatc tcatcaccta gtttaacaac
      181 atcttcgact ttattgacac gatcgtttgc taattgggaa atatggacaa gtccatcttt
      241 tcctttgatc aagttgacga atgcaccgaa tttttcgata cggacaactt tccctagata
      301 aacttcacca actttgactt ctttggtcaa atcttcgatg atcttgatag cttttttgat
      361 catttctgca tcagctgaag caatacttac ttttccatct tgatcgatat cgattttcac
      421 accagtttca tcgatgattc cgttgatcgt atcgccgcct ttaccgataa catctttgat
      481 tttagcaggt tcgatctgga tcatttcgat ttttggtgca tattggctta attcttcacg
      541 tggagcagca atcgttgaag tcaattcttc taagatttcc atacgtgctt gttttgcttg
      601 agttaatgct tcagttagga tttgttctgt gattccttga atcttgatat ccatctgtaa
      661 agcagtgata ccatcttttg taccggcaac tttaaagtcc atatcgccaa gatgatcttc
      721 cagcccttgg atatcagtta ggatcgtata gttttcacca tcagaaacaa gtcccatcgc
      781 aataccggcg actggcgctt tgattggtac acctgcatcc atcaatgcta atgtgccagc
      841 acagatactt gcttgggaag aagaaccatt tgattccaat acttctgcta ctaaacggat
      901 cgtataagga aattcttctt ctgaagggat tacttgtgct aacgcacgtt cacctaatgc
      961 gccatgtccg atttcccgac gccctggaga gcctgcacgt cctgtagaac caacagaaaa
     1021 ttgagggaaa ttgtagtggt ggatgaatcg tttgctgact tccaccccta aaccatcaat
     1081 gatttggtgc tctcctagag gtgcgagtgt acaagctgat aaagcttgtg tttgtcctcg
     1141 tgtgaacaaa cctgatccat gtacacgtgg caataagctg acctctgatg acaatggacg
     1201 gatttcatcc aatttacgcc cgtcaggacg gatcttatcg atggtgatca attcacgtac
     1261 gacatctttt tcaagatctt ctgtgatttg tttaacttct ttcaacaagt gatcttcatc
     1321 ttcatgacct gcaaatttct cagcataagc ttcgcggata tcgatcttca cctgctcgat
     1381 attatcttcg cgagcaagtt tttcttcagt cattactgct gctttcattg tagcgtaaga
     1441 agcatcaaag atttcttttt tcaatacttc atcaacttgc agtaaagtca cttccatttt
     1501 ttctttccct actgcttgaa cgatttcttc ttggaaagca actaactctt tgatagcgtc
     1561 aaaaccaaac agcaatgcac caagcatgtc ttcttctgaa acttctttcg cgccgctttc
     1621 aaccatattg atggcttgtt tcgttccggc aacagtcaat tcgatatctg tttgttctgc
     1681 ttgttctact gttggattca agacgtactc gccatttaca cgaccaactt ctacgcctgc
     1741 gattgggccg tcaaatggga tatctgagat tgatagtgct aaagaagaac ctagcattgc
     1801 tgccatagct ggcgagcagt ctgtttctac actcatcacg atgttcgtta cttggacttc
     1861 attacggaag ccttctgcaa acatcgggcg aattggacgg tcgatcaaac gggcagtcaa
     1921 tgttgcttcc gtacttggac ggccttctcg tttgatgaaa cctccaggga ttttccctgc
     1981 tgcatacatt ttttcttcat aattgatcgt taatgggaaa aagtctgtat cttttgcttc
     2041 tttagaggcc acagctgcac ttaagactac tgtatctcca taacggacta aaacggctcc
     2101 gtttgcttgt tttgctagct gaccgacttc gatttgtaag ggacggccgc cccatgtcgt
     2161 tttgaatact tgtttttctg acataacttc tccttttcct tctcgtgaaa gcaagacgct
     2221 actaaacaaa tgaaaactgc cgaaagccgt cttttgagca gctttcattt ggttagtagg
     2281 agtccatcta gcttgatcac ggaattttac ttaattgata tacgagtaaa aaaagtgaga
     2341 ttcatgtgaa tcccactctt tttgactaaa attaacgacg caagcctaaa cgtttgatca
     2401 attcgcggta acgttgaacg tcagttttac gtaagtaagc taacaagtta cggcggtgac
     2461 caactttttt cattaggccg cggtatgaat gatggtcttt tttgtgcacg cgtgcatgtt
     2521 cattcaagtg gttgatttct tcagttaata cagcgatttg tacttctgga gaaccagtat
     2581 ccccttcatg gcgagcgtat tcgttgatga tttcattctt gcgttctttt gaaattgcca
     2641 tttctttcac ctctttcgat tattcccctg cgactgagta aaacgttggt gattcgctta
     2701 accaagtggc aggtcatgtg cctagagcac acagctatac tttacctgaa accgggttga
     2761 aaatcaagta gttaatcatt aagaaaaggt agttttcaaa caatcttact actacttttc
     2821 catttttgtg gtaaactata atgcgaaaat agacttgaaa cacagtcttt taagaaaggg
     2881 gtacatattc catgattaca atggatgata tcattcgtga aggaaatccg accttacgcg
     2941 aagttgcaaa agaagtttct cttccattaa gcgaagaaga tatctcttta ggaaaagaga
     3001 tgcttgagtt cttaaaaaac agccaagatc caatcaaagc tgaagaacta cacttacgag
     3061 gcggtgttgg cttagctgct cctcaattag atatttctaa aagaatcatt gctgttcacg
     3121 taccaagtcc tgacccagag gcagatggac cttctctaag tactgttatg tataatccta
     3181 aaattttgag ccattctgtt caagatgctt gtttagggga aggtgaaggc tgtttatctg
     3241 ttgatcgaga agtaccagga tatgttgttc gccatgccaa aatcactgtg tcttattatg
     3301 atatgaatgg tgaaaaacac aagattcgtt tgaagaatta tgaatcgatc gtcgttcaac
     3361 atgaaatcga ccatatcaac ggtgtgatgt tctatgacca catcaacgat caaaatccat
     3421 ttgcactaaa agaaggcgta ttggtgatcg agtagagaat tggcaggttc cttcgatact
     3481 atgctacaag aaagctttat aaaaaagggg agtgctcaaa agctgcactt ccctttttta
     3541 tgtttctcca ctaatcgaaa tttagataga catctagaaa tcgcaattcc aaaaaattct
     3601 gtgtattgga tacgcatctt ttcttatttt ttcttatttc ttccatatgg gtgtttatct
     3661 cagagatgat agaatagttt ctaggataaa aagtagcagc tacgatgtta tccttcgtag
     3721 aggattcctg cttcagctag tttttgtttg atgtttttca gcatatgctt ttctgggtag
     3781 cggatcgtat gcagatggat gccgcctgtt aattcggata acaaagtagc attacttttt
     3841 tgataacgtc tcataaagga atcgatatct tgtggcgttt tgatgtgcaa agttcctgct
     3901 aactcaccgt aaaggggatg ttccacaatt acatcaatga tttcaccacc attttcaacg
     3961 attagtctca gttcttcttc tgtttgatct tttccatgct ggacagcgat tttactgatg
     4021 tgcccagtat tggtttcttc tgtttcgagg atataacctc ttgctgttgc cacgatactt
     4081 ttacctgcag ctcgtagtaa agcaatatcg cctacaatga tctgacggct gactccaaaa
     4141 gttttcgcaa acttgctagc actcactggt ttttctgtat ttgccaaggt ttgcatgatt
     4201 tgttttcgcc gtgtctctcc ttcgatcaca cttattcctc cttcattttg ttttggtatt
     4261 cgcgcgagtg tttcatcaaa tcaacatacg tgtctgttat tgtttcctta tatcccttat
     4321 ttttcagcag actaacaact ttatccaata cttgctgctc tctttttgca tcataaactg
     4381 cttggttttc ttcttttttc acttgtccta ttttagttac cgtatccatt cttttttcta
     4441 ataaagcaac caactctcga tcgatcatat cgatcttatg acgtaatgtt tcaagagact
     4501 cttctgttag ttcttcctct actgtcttga tgatacgagc aggatttcct gcaatcacta
     4561 cattgtcggg aaaagatttg gtcacaaccg aacctgcgcc gactactacg ttgtccccta
     4621 gagtgacacc cggcgtgacg ataactcctc cgcctaacca aacattatta ccgattttaa
     4681 tgggttttgc gtactccaga ccgctgttac gtttcactgg atgtagtgga tgcgtcgccg
     4741 tgtaaagctg gacattcgga gcgaacatgc agttatcacc gatttcaatc gtgctgacat
     4801 ctaaaaatgt acaattgaaa tttgcataaa aattctcccc aacaaatata ttatatccgt
     4861 aatcaaaatt gataactggc tccatataga tttttttccc tgtacggcca aaagtctctt
     4921 tcaacaattg ggatcgcagt tccgctgatt ctgcttggtt gatgatttgg ctttgacttc
     4981 ttgcaaacat acggtcggca accagctcag gatcattggc aaaataaagc tcgcctgcaa
     5041 tcattttttc tttttcggtt ttgaattcag tcataatctg ctcctaattc ttgtattttt
     5101 ttgaaaacaa ccgctccgtc ttcttgaata aacgtataag gctccgctaa ataaacaggc
     5161 gtttgattga ttactaagat tttggctgac ggctggcgaa actgtaccag atcacaaaac
     5221 ggatgaactt gaaaactcgt tccaacaatg acgatcagct ctgcttggct gacggcataa
     5281 gccgcctgct ccatgactcc atcttgaaac ccttcaccat ataacacgat gtctgggcgt
     5341 atttgtcctc cgcatgcttg atgtttgtcg ctgtgaagat attctttcca gtctaccgtt
     5401 tgttgacatt ttcgacaatg acattgatac agactcccat gaaaattgac gagttgcggg
     5461 cttttagcct ttgcgtgcaa accgtcgatg ttttgggaaa caacccaaac atttttttct
     5521 ttcgctagat gtgctatttc caaatgaatg atattgggtt ttgcttcagg atgataaatc
     5581 tttttgacaa attgatagaa tttatccggt tcattcacca ttgcttgatg actcagcaag
     5641 tattccggct gctccatccc gtggtagatt cctgacaaag atcgatagtc tggcactcca
     5701 gaaggtgtgg atacccctgc accggtcaaa aatgtaattt gctgataagc taatatctct
     5761 tttgctgctt cgtctactgt taattgtttc atgttttgtc acctcacttt ccattatagc
     5821 ccatactcga acaaatttat tgttgaaaag tcagaaaaat gggaataaaa aagtgttatg
     5881 ggcatttgag caatttatca caatgttcac tgacaaaaga atgaaaacgt gttacaatac
     5941 tgctgtataa aagtgaggtg aatcgatatg ttaccattct ttatttattg gggcgttatt
     6001 ctggcttgta tcgcctggtt agcgttaagc gtttatttct ctgtttttta tcttgtacgc
     6061 aaagaaaatg gaaatctttg ggcttttgct ttattcaacg tgattgccgc aattgtttta
     6121 gccattacat tagccgtata tcgaacatgg ggctggggaa tcacccaata ctcaagtttg
     6181 atttatctaa tattagccat ttatggcgtc gttgttcttt tgcaagcgat cctaggacgt
     6241 gagccaaaga aagcggcagc ttaaatgatc gagatggaac tggacacagg aaattttctg
     6301 ttctagttct ttttttattg tattacatac atttttgtgt ataattatac tatcgtttgc
     6361 aatggataga tgattcacat tttgattgtt ttttcggtaa aagtaaagat ttctatattt
     6421 atcagtattt tttcaaaaac cagcagttat agattgacaa agcctctcga gtatcgtaaa
     6481 ctttttttgt ataaataact gaatacgaga tgcctcaatc aatgaggtag aggtcgcgag
     6541 aatcattaac cctgtggagt gaagcaaaca tggagaagca ggaataggga ggatcgccga
     6601 aatgtgcact tttttgcttg gagtgtatgt tgggacgcaa gttaagagct tgcggactgt
     6661 cttagcagtg atgctaagtt gcgctatgtt ttgtgagagg tttatcacct tatggaattt
     6721 tagcggcctt tttatatcta taaatcggtc gctttttatt ttttcagata aagctttacc
     6781 gcatttcatc tagaccgctc gtgtatcata taatcagaaa gggagaaaaa aatgaaaaaa
     6841 aatatggttt cagttgccgt actatttgct tttgtcttct cactattcag tgtgggatct
     6901 ctggcaaatg cagatgaaaa aacaccgagc gatgagtttc gcgtaggtat ggaggctggg
     6961 tatgcaccct tcaactggtc tcaacaaaca gatgagaata atgccttgcc aattcaaggc
     7021 caaaatagtt acgccggcgg ttatgatgtt caaatcgcta aaaaagtagc agatggttta
     7081 gggaaaaaat tagtcatcgt ccaaacgaaa tgggatggtc tggctcctgc ccttcaatca
     7141 ggcaagatcg acgcgatcat cgccggaatg agccctactg ctgaacgacg aaaagaaatt
     7201 gattttaccg atccttatta cgaatcacaa ttagtagtag tagttcaaaa agatagcaaa
     7261 tatgcaaaag caaaaagttt agcagatctt tctggagcaa agatcactgc tcagttaaat
     7321 acattccact atactgtgat cgatcagata ccagatgtgc agaaacaaca agcaatggat
     7381 aatttctcag caatgagaac agctttagct tctggaatga tcgatggtta tgtcagcgaa
     7441 cgtccggaag gtgtaacggc aactagtgtg aacaaagact tggaaatgct tgaattcagc
     7501 ggtgcagatg gcttccagac aaacccggaa gatgttcaga tttctgttgg tatgcgtaaa
     7561 ggcgacccgg atatccaaaa agtaaaccag attctttctg gcatctctaa cgatgaacga
     7621 accaaaatta tggaccaagc agtcaaagat cagcctgctg caactgatag tgaagaaaaa
     7681 cctggcatca tttctgattt cactacgatt tggaaacaat acggcaatat gttcttacga
     7741 ggtgctggac ttaccttgtt catcgcattg atcgggacag ttgtcggtac tacacttgga
     7801 ttactgattg gtgtcttccg ttcggtacct gaatcaggaa atgcactgac tcgcttcttc
     7861 caaaaaatcg gaaatgcact tctttctatc tatgtcgaag tattccgcgg aacaccgatg
     7921 atggtacaag caatggtcat cttctacggt ctggcactcg cttttggcat ttcattgaac
     7981 cgaacgattg ccgctttgtt tattgtctct ctcaatactg gagcttacat gacagagatc
     8041 gttcgtgggg ggatttttgc agtagatgaa gggcagttcg aagctgcgca agccattggt
     8101 atgacccatg gccaaacgat gagaaaagtt gttattccac aagttttacg gaatatcttg
     8161 ccagcaacag gaaatgagtt tgttatcaat atcaaagata cagcagtttt aagtgtaatc
     8221 ggcgtagctg atttgttctt ccaaggaaac tctgcttccg gtgcgaattt ccaattcttc
     8281 caaacattta cgatcgtcgg gatcatctac ttagtcatga cctttacgat caccagaatc
     8341 ctgcgggcgg tagaaaagaa aatcgatggt ccttcagctt ataccaagct tgaagaaatc
     8401 gacaattcaa atttgcagga ataaggagga agaatcatga atgaaaaaat tattgaaatc
     8461 aaacatctaa gaaaaagctt cggtgaaaat gaagtattga aagatatcag cgtaacagtc
     8521 aataaaggtg aagttgtgac gatcatcggt tcttccggtt ctggtaaatc tactttcttg
     8581 cgctgtatca atttactgga aaaaccaaca ggcggcgaga tcatttataa tggagaaaat
     8641 gtattagctc ctaaatataa tttacctaaa tatcgaacga atctaggtat ggttttccaa
     8701 tccttcaacc tgttcaacaa tatgaatgtc ttggaaaact gtatgtctgg tcaaacaact
     8761 gtcctgaaac gagataaaga aactgccaaa aaagtagcaa tcgaaaatct ggaaaaagtt
     8821 ggtatggatc gttatatcga agccaaacca gcacaattat ctggtggaca gaaacagcgt
     8881 gtcgcaattg ctcgggcatt atcaatgaat ccagatgtat tgctattcga cgaacctact
     8941 tcggcgttag accctgaaat ggttggcgaa gtgcttaata cgatcaaaga cttagcacat
     9001 actggcttga caatgcttat cgtgacgcat gagatggaat ttgcaaaaga tgtatctgac
     9061 cgtgtcattt ttatggataa aggtgtgatt gcagaagaag gtacaccaga agatatcttt
     9121 gtccatccga aagaagaacg tacaaaagaa ttcctttcac gtattttaaa tgcttaggat
     9181 caatagctga ttgggacaaa gagctgcggt gtttaccgca gttcttttt
//
LOCUS       DASHHB010000084         9108 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.088, whole genome shotgun sequence.
ACCESSION   DASHHB010000084 DASHHB010000000
VERSION     DASHHB010000084.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 9108)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9108)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9108
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.088"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..649)
                     /locus_tag="U2K71_002542"
     CDS             complement(<1..649)
                     /locus_tag="U2K71_002542"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HEM7316560.1"
                     /translation="MKGDVIVEIKVLFENSRINLNFKSGHGLSLLINYSGKKYLFDAG
                     ANKKLVYNATKMGETLNDLEAVIISHGHSDHTGGLRYLKSGNAPIYTAEGVTLPHYLK
                     ILTTFLYVGIPKNEILKMHENLHTIKTKTELAPSFHIIPLGKANSTTKNLYKKMDDKF
                     VYDDFSDELLVVIEQEDGLIVFTGCSHHGIIDMMEIALETFPTKPIKYLIGGFHMI"
     gene            974..1390
                     /locus_tag="U2K71_002543"
     CDS             974..1390
                     /locus_tag="U2K71_002543"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS fructose transporter subunit IIA"
                     /protein_id="HEM7316561.1"
                     /translation="MATIILASHGEFAQGLKQTATMIIGDSVPIHALSAFRDEDESIL
                     VQIKKLLATIDIEETYILTDILGGSVNNDMLTIIKEYEGLQLITGMNLPLVISIATYN
                     GKIGESELEKLIKESQQSVIDCRKLLVEQLTGGDDL"
     gene            1387..1857
                     /locus_tag="U2K71_002544"
     CDS             1387..1857
                     /locus_tag="U2K71_002544"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIB"
                     /protein_id="HEM7316562.1"
                     /translation="MIKLVRIDHRLLHGQVVFSWSKSLNINRIIIANKEAANDEFKKM
                     SLNLSKPTGIKLTIFSVEQLLEKMPKIETLNENIMLIFGNTKEIRQFCEAYPKVQEIN
                     YGGIIKKEGSKQFSNAIFLNESEQEDAKKLKALGIKQFIQQVPTSKKEDLNTML"
     gene            1871..2644
                     /locus_tag="U2K71_002545"
     CDS             1871..2644
                     /locus_tag="U2K71_002545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7316563.1"
                     /translation="MLVQSIGLGLIGVFCILDSRLLGRMNFERPLIVSTLVGLVLGDM
                     EKGLIVGASLELMSLGLVNIGAAAPPDMNMASIIATAFAILSNANAEAALTIAIPISV
                     LGQILGILMRTILSNLTHVADRFIEQGKFSQARRIHILYGSGLYALMYFIPIFLAIYF
                     GTNLVSQIVDAIPEWLTNGLTVASKILPAYGFALLLQTMLTKKMVPFLLLGFLITAYS
                     GLGIIGIAAFACIVAFVMVQVQGKNSNGNQNEPDPLDDL"
     gene            2658..3470
                     /locus_tag="U2K71_002546"
     CDS             2658..3470
                     /locus_tag="U2K71_002546"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS system mannose/fructose/sorbose family
                     transporter subunit IID"
                     /protein_id="HEM7316564.1"
                     /translation="MKEKDQNAWKYRQFLWRSWAIQASWNYERQMNMGFMYGIAPILD
                     NIYKNPEDEAMKKEAYERHMMYYNCTPQTSAFVLGLTASMEEQYYEDQENFNPESISA
                     VKTSLMGPLSGVGDSFFQGTIRVLAFGLGINLAQQGSILGPILAMLISFVPSFLVTYY
                     GGKIGYNMGNKYLSKLYQEGLMDKVMYVCSIVGLMVIGSMIASMIGITTPIQMGKAFV
                     LQDVLDSIMPQFLPLGLTFFMYWLLQKKVKTGWMLAICLVGGIIFSLLGILQ"
     gene            3524..4489
                     /locus_tag="U2K71_002547"
     CDS             3524..4489
                     /locus_tag="U2K71_002547"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM7316565.1"
                     /translation="MNSKEIVKEIVAKKEVKSIVFVGCGASKADLYPAKYFLDQNAKQ
                     LRISHYTASEFNYATPASVDESTVVISASLGGTTPETVEANAVAKEKGATVISLTRSI
                     DSPLTKDADYVIYHRFAENYAAKLEKTGYCLELAVELLHQVEGYDHYEAMQEGFATIY
                     DLAQKSAESARKDAKKFAERFKDEKVIYVMSSGATHEVAYSTSICLMMEMQWINSGTF
                     HSGEFFHGPFEIVDKDVPFILLMNDGKTRNIDARALTFLERFDTKTEVVDALDYGLSS
                     HIAKEVLDYFNPFVITAVFRVYAEELSEAREHPLTKRRYMWKLTY"
     gene            4605..7328
                     /locus_tag="U2K71_002548"
     CDS             4605..7328
                     /locus_tag="U2K71_002548"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma 54-interacting transcriptional regulator"
                     /protein_id="HEM7316566.1"
                     /translation="MQRLNDYLYQLTLLLKSTPSDNYSARSISQALQLNRSTISSYLN
                     EGVREGLFIKVKSYPVLFLHRTALEELHITLNNSEIESIESLLTISQKPALDQVIGSK
                     GSLKEAIDQIKTAVLYPGKGLPLLLIGASGSGKTFLANKIYEYAVEEKVIHTSAPFID
                     YNCAQYVSNPELLSSALFGYTKGAFTGASQEHTGLLEKADGGVLFLDEVHRLSEEGQE
                     KLFTFMDTGEFSPMGDNSIRKKADVRLVFATTENIYTTFLPTFLRRLPVIVNLPRFQQ
                     RPSFERLSLIDEFFVSESKILAKELSVSDALIHFLMNSDFEGNVGNIKNIIKYACGNA
                     YIHQKSQQTIQVKLLDMPLEYSYKFKEQLNKPRKKRADRIYYPEETRQVQLERNNQLF
                     VQFFDQLLTGFSEVIENNKDIKLFLEEMVTKVTQVMDTLIFQEDYEKEQSLYAILNYH
                     IRQSIDFMRESYGFEQDGNRVVALANYLYVKDNQVIFENSLQEKKTKDQLNEFFANVL
                     ETPYWYAKKILSLLSECLDQPVYEEDIFFITLYFYSLNVTNVKTGIKSIVLAHGYSTA
                     SSMANVANRMLNKNVFQAYDMPLDITLEKIEAKLIQFLGDYRTDSGLILLVDMGSLNQ
                     LGGLLVNHLNGPLLIIDHINTPLLLEVGQQVLNNKSITEIGAEIETSLTFKKQLLLPK
                     KKKKKAIITCCYTGIGSATQIQEILTKCLKDAVRELVIIPYDYKKLLENKNFETPFQL
                     YDVIMIVGTEDPKINQIPYIGLDRLINGEAIGEFVHYLEKELTIEVNDLQKELIFNFS
                     ISKIVENLTILDPDKVLSFVQKAIKELSKLLNFDFSNNQLFLLYLHCSCMIERIVRKE
                     SVDEQEDIEEYMQANGEIMDKIHSAFFEIEREYTIEIPDLELRLLSDIVQG"
     gene            7341..7973
                     /locus_tag="U2K71_002549"
     CDS             7341..7973
                     /locus_tag="U2K71_002549"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010729807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HEM7316567.1"
                     /translation="MTMGVIFDMDGVLIDSETFYFERRMNFFQELEIDPGSNQIDDYV
                     GKTEAAIWETLVPEDKSLREELYPKYVDYRKTHPIDFAKDLRKEVKPLLDFLKKRDIP
                     LALASSSPVVEIESMLIQCELTEYFSFVISGESLKQSKPHPEIYLKAKQQLDCDFFIA
                     VEDSTIGIKSAKAANIYTVALKQPMQIDQSEADIQIESLQELEQIVLEKG"
     gene            8178..8405
                     /locus_tag="U2K71_002550"
     CDS             8178..8405
                     /locus_tag="U2K71_002550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011837604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ADP-ribosylglycohydrolase family protein"
                     /protein_id="HEM7316568.1"
                     /translation="MLQQNQNTNSYQAAILTAVNLGGDTDTTAAVTGALAGILYGGEV
                     IPENWRKSLQNDQLLKTISQRFCDTVLSQRI"
     gene            8474..8740
                     /locus_tag="U2K71_002551"
     CDS             8474..8740
                     /locus_tag="U2K71_002551"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002383791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEM7316569.1"
                     /translation="MLVQMMNGGRIMSQSTITFRIPDDEKELVLEYAKAHNSSLTELY
                     RNAVLDKIEDEIDLRTLRSAIEVSKEKKESGISQEEMEKLLNEL"
     gene            8730..8999
                     /locus_tag="U2K71_002552"
     CDS             8730..8999
                     /locus_tag="U2K71_002552"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM7316570.1"
                     /translation="MNYKVIYMPQAQKQLKKMDRNQAKIIVAWIRKNLENTTDPRQHG
                     KGLTGNKSGEWRYRIGNYRILANICDSEIEIQIFSIGHRSTIYKR"
BASE COUNT         3129 a         1378 c         1793 g         2808 t
ORIGIN      
        1 caatcatatg gaagccaccg attaggtatt ttatgggttt agtcggaaat gtttcaagag
       61 caatttccat catatcgata atgccatgat gactacagcc agtaaagaca atcagaccgt
      121 cttcttgctc aattacgacc aataattcat ctgaaaaatc atcataaaca aatttatcat
      181 ccatcttttt gtataaattc ttggtggtcg agtttgcttt gcctaggggg atgatatgga
      241 agctcggagc aagttctgtt ttcgttttta ttgtatgtaa attctcatgc attttaagga
      301 tttcattttt aggaatacca acatacagaa acgtggttaa aatctttaaa taatggggta
      361 aagttacacc ttcagctgta taaatagggg cgtttcctga ttttaaatat cttaacccgc
      421 cggtgtggtc agaatgaccg tgtgaaataa tcactgcctc cagatcattc aaagtttccc
      481 ccattttagt cgcattatag actaattttt tattagcgcc agcatcgaag agatatttct
      541 tccctgagta gttaattaag agagataacc catgtccact tttaaagttc aaatttattc
      601 ttgagttttc aaataaaacc ttgatttcca ctataacatc tcctttcaca tgaacgtgta
      661 ttcatatatt gtagcacatt aagttctatt ttttgttgta attaccgaaa aaaatattaa
      721 tggacgcttt ttacgcttta tttcgtatgt ggaagaaaat ttcttaggat gtggcggggg
      781 attggggcaa agctaaattc caattacagt gatcatttaa ttagacacct ttaaataccc
      841 acactttcat caacactata atttagggaa agaaaagtgt tggcatgata tagaaaaacc
      901 tttaattaac agcgtttcct aaaattggca cgcatcttgc ttatagaaaa gcgtaaggat
      961 aggaggaatg aaaatggcaa ctataattct tgccagccat ggggaatttg ctcaaggatt
     1021 aaagcaaaca gctacaatga ttattggaga tagtgtacca attcatgcct tgtcagcatt
     1081 tagagatgaa gatgaatcta ttttggtaca aataaaaaaa cttttagcca caatagatat
     1141 agaagaaacc tacattttaa ctgatattct aggtggtagt gtgaataatg atatgctcac
     1201 tattattaaa gagtatgagg gattgcaatt aattactgga atgaacttac ctttagttat
     1261 ttctattgca acctataatg gaaaaattgg ggaaagtgag ctagaaaagc ttattaaaga
     1321 gagtcaacaa tcagtgattg attgtagaaa attattagta gaacaactta ctggaggaga
     1381 tgacctatga ttaagttagt acgtattgac catcgtttgt tgcacggaca ggtagtattt
     1441 tcttggagta aatcattaaa tatcaatcgt attattattg ccaataaaga ggcagcgaat
     1501 gatgaattca aaaaaatgtc tttgaattta tctaaaccga ctggcattaa attaacaatt
     1561 ttttcagttg aacagttact agaaaaaatg cctaaaatcg aaacacttaa cgaaaatatc
     1621 atgcttattt ttggtaatac aaaagaaatt cgtcaatttt gtgaagccta tcctaaagta
     1681 caagaaatca actatggagg gataatcaaa aaagaaggtt ccaagcaatt tagtaatgcc
     1741 attttcttga atgaatctga gcaagaagat gccaaaaaat taaaagctct tggcattaaa
     1801 caatttattc aacaggtacc aacttctaaa aaagaagatt tgaatacaat gttataaagg
     1861 aggaatgaaa atgctcgttc agtcaattgg tttagggtta atcggtgttt tttgtatttt
     1921 agactcaagg cttttaggaa gaatgaattt tgaacgaccg ttgattgtca gtacgcttgt
     1981 tggtttggta cttggagaca tggaaaaagg attaattgtt ggagcctctt tggaattgat
     2041 gtccttagga ttagtaaata tcggagctgc tgcaccacct gatatgaata tggcatcaat
     2101 tattgctaca gcctttgcca ttttatcaaa tgcgaatgca gaagcagcat taacgattgc
     2161 cattccaatt tcagtgttag gtcaaatact aggtatctta atgcggacga ttttgtcaaa
     2221 tttgactcac gttgcggatc gctttatcga acaagggaaa tttagccaag caaggagaat
     2281 tcatatactt tatggctcgg gattatatgc tctcatgtat tttattccta tttttctagc
     2341 aatttatttt ggaacgaatc ttgtttctca gattgttgat gcgattcctg agtggctaac
     2401 aaatggctta acggttgcaa gtaaaatttt accagcatat gggtttgcgt tgttattaca
     2461 aacgatgtta acgaaaaaaa tggtgccatt tttattactt ggatttttaa ttactgctta
     2521 ttctggattg ggaatcattg gtattgcagc cttcgcatgt attgttgcgt ttgtcatggt
     2581 tcaagttcaa ggtaaaaact caaatggtaa ccaaaatgag ccagatccat tagatgactt
     2641 ataaggaggt agattaagtg aaagagaaag atcaaaatgc atggaaatat cggcagttct
     2701 tatggcgttc atgggcaatt caagcctcat ggaactatga acgacaaatg aacatgggtt
     2761 ttatgtacgg aattgcacca attctagata acatttacaa gaatccagaa gatgaagcaa
     2821 tgaagaaaga agcttacgag cggcatatga tgtactacaa ctgtacccct caaacaagtg
     2881 catttgtgtt agggttaact gcttctatgg aggagcaata ctacgaagac caagaaaact
     2941 ttaatccgga atcaatcagt gcagtgaaga cttctctgat gggaccgcta tctggtgttg
     3001 gtgattcttt ctttcaagga acgattcggg tattagcctt tggactagga attaatttag
     3061 cccaacaagg tagtatttta ggtccaattc tagcaatgtt aatttccttt gtaccttcct
     3121 ttttagttac ttactatggt gggaaaattg gctataacat ggggaataaa tacttgtcta
     3181 aactgtatca agaaggtttg atggataaag taatgtatgt ttgctccatt gtggggttga
     3241 tggtcattgg ttcgatgatt gccagcatga tcgggattac aacaccaatt caaatgggta
     3301 aagcttttgt cttacaagat gtattagata gtattatgcc acaattttta ccattaggat
     3361 taaccttctt catgtactgg ttattgcaga aaaaggtcaa aacaggatgg atgttggcaa
     3421 tctgtttagt cggaggaatt atttttagtt tgttaggtat cttacaataa gggaataacc
     3481 aataaatttg gttgaataat ataaataagg aggaattcaa ttgatgaatt caaaagaaat
     3541 tgttaaagaa atcgtagcga aaaaagaggt aaaatcaatc gtatttgttg gctgtggcgc
     3601 ttcaaaagcg gacctttatc ctgcaaagta ttttctagac caaaatgcga agcagttacg
     3661 gattagtcat tacacagcca gcgaatttaa ctatgcaaca ccagcatctg tagatgagtc
     3721 aactgtcgtt atttcagctt ctttaggagg tacgactcca gaaacagttg aagctaacgc
     3781 agtagctaaa gaaaaaggtg ctacagttat tagtctaaca cgaagcattg actcaccgtt
     3841 aacaaaagat gcagattatg taatttatca tcgatttgca gagaactatg ctgctaaatt
     3901 agaaaaaaca ggctactgtt tagaattggc agtagaactt ttacatcaag tagaaggtta
     3961 tgaccattat gaagcaatgc aagaaggatt tgctactatc tatgatcttg cccaaaaaag
     4021 tgcagaatca gctcgtaaag atgcgaagaa atttgctgaa cgctttaaag atgaaaaagt
     4081 tatttatgta atgagtagtg gtgctacaca tgaagtagca tactcaacat caatttgctt
     4141 gatgatggaa atgcaatgga ttaattctgg taccttccat tcaggagaat ttttccacgg
     4201 accattcgaa attgtggata aagacgtacc gtttatatta ttaatgaatg atggaaaaac
     4261 tagaaatata gatgctcgag cattaacttt ccttgaacgc tttgatacga aaacagaagt
     4321 tgtagatgcc ttggattatg gcttgtcttc acatattgct aaagaagtct tagactactt
     4381 taacccattc gtgattactg cagtatttag agtttatgca gaagaattgt cagaagcaag
     4441 agaacatcca ttaactaaga gacgttatat gtggaaacta acttattaaa aaatagatca
     4501 aactaaataa ggtggcttga gaaattaagc caccttgttt tcttcaatgt gattttatac
     4561 atatggtaaa ataactagta catattgtga aagcaggtgg caagatgcag cgattaaatg
     4621 actatcttta tcaactaaca ttgcttttga aatctacgcc ttcagataat tattcagctc
     4681 gcagtatttc gcaagcttta cagttgaata gaagtacaat cagtagctac ttaaatgaag
     4741 gtgttcgtga agggcttttc ataaaagtaa aaagttaccc ggttttattt ttacatagaa
     4801 cagctcttga agaactacat atcacactga ataactccga aatagaatca attgaaagct
     4861 tgttaacaat ttctcaaaaa cccgcattag atcaagtaat tggctctaaa ggaagtttga
     4921 aagaagcaat tgatcaaatt aaaacggcag tcttatatcc tggaaaaggg ttgcctttat
     4981 tattgatcgg cgctagtgga tcagggaaaa cctttctagc gaataaaata tatgaatatg
     5041 cagtagaaga aaaggtcatt cacacatcgg cacctttcat agattataat tgtgcacaat
     5101 atgtgagcaa tccagagtta ctatcctctg ctttatttgg ctacacgaaa ggagcattta
     5161 ccggagcaag ccaggagcat actggtttgc tagaaaaagc cgatggagga gtattgtttt
     5221 tagatgaagt gcatcgctta tctgaagaag ggcaagagaa attatttaca tttatggata
     5281 cgggtgaatt ctctcctatg ggggataata gtattcggaa aaaagccgat gttcgattag
     5341 tctttgcaac aacagaaaat atatatacga ctttcttacc aacttttctt aggagattgc
     5401 cagttattgt taatctacct agatttcaac aacgtccgtc gtttgaacgt ctgtcattga
     5461 ttgatgaatt ttttgtttct gagagtaaaa ttttagctaa ggaactatct gtttctgatg
     5521 cattgattca tttcttaatg aatagtgatt ttgaaggtaa cgtggggaat attaaaaata
     5581 ttatcaagta tgcttgcgga aacgcatata tccaccagaa gagtcagcaa acaattcaag
     5641 ttaaattatt ggatatgcct ttagagtaca gttataaatt caaggagcaa ctaaataaac
     5701 ccagaaagaa aagagcagat cgaatctact atccagaaga aacacgacaa gtgcaactag
     5761 aaagaaacaa tcagcttttt gttcaatttt ttgaccaatt acttactgga ttctctgaag
     5821 tgattgaaaa taacaaggat attaaactat ttttagaaga aatggttacc aaggtaacac
     5881 aagtaatgga tacgcttatt tttcaagagg attacgaaaa agagcagtct ttatacgcca
     5941 ttctaaacta tcatattcgt caaagtattg attttatgcg agaaagctac gggtttgaac
     6001 aagatggtaa ccgggtagtc gctttggcca actatctata tgtcaaggat aatcaagtga
     6061 tttttgaaaa tagtcttcaa gaaaagaaaa ccaaagatca gcttaacgaa ttttttgcta
     6121 atgttcttga aaccccttat tggtatgcaa aaaaaatctt aagcctttta tctgagtgtt
     6181 tagatcaacc tgtgtatgaa gaggacattt tctttattac gctatatttt tatagtttga
     6241 atgtcacgaa tgttaaaaca ggtattaaga gtattgtgct agctcatggt tattctacag
     6301 ctagtagtat ggcgaatgtt gccaaccgta tgctgaataa gaacgttttt caagcttatg
     6361 atatgccttt agatattaca ttagagaaaa ttgaagcaaa attgattcaa ttccttggag
     6421 attatcgaac agattcaggc ttgattttat tggtagatat gggttcacta aaccaactag
     6481 gcggtttatt agtgaatcat ctaaatggac cactgttaat cattgatcat atcaatacac
     6541 cgttattatt ggaagtcggc caacaagtat taaacaataa aagtatcacc gaaataggag
     6601 cagaaattga aacaagtctt actttcaaaa agcagttact acttcccaag aaaaagaaaa
     6661 agaaagccat cattacttgt tgttatacag ggattggtag tgcgacccag attcaggaga
     6721 ttttaacgaa atgcttgaaa gatgcagtta gagaattagt aattattccg tatgattaca
     6781 aaaagctgtt agaaaacaag aattttgaga ctccttttca attatacgat gtcatcatga
     6841 ttgtaggtac agaagatccc aaaattaatc agattccata tattggatta gatcgcctga
     6901 ttaatgggga agcaattggc gaatttgttc attacttgga aaaagagctg actattgaag
     6961 tgaacgattt acaaaaagaa ttaattttca atttttcaat tagtaaaatt gtcgaaaatt
     7021 taaccatttt ggatccagat aaggtgctat cttttgtaca aaaagcaatt aaagaattat
     7081 cgaagttatt aaattttgat ttttctaata atcagctgtt tttgttgtac cttcactgta
     7141 gttgtatgat tgaacgaatt gtacgtaaag aaagtgtgga tgagcaagag gacattgaag
     7201 aatatatgca agcaaatgga gaaataatgg ataaaattca tagcgctttt tttgagattg
     7261 aaagggaata cacaattgaa ataccagatt tagagttgcg attattgagc gatattgttc
     7321 aaggatagga gtgaattcga atgacaatgg gagtaatttt tgatatggat ggggtactca
     7381 tagacagcga gacattttat tttgagagaa gaatgaactt ctttcaagaa ctggagatag
     7441 atccaggatc aaaccagatt gatgattatg tggggaaaac agaagctgca atatgggaaa
     7501 cattagttcc tgaggacaaa tcattaagag aggaacttta tccgaaatat gtagattatc
     7561 gaaaaacgca tccaattgat ttcgctaaag atttacgaaa agaagtgaaa ccgttacttg
     7621 acttcctcaa gaagcgggat ataccattgg cactggcatc ttcttctcca gttgttgaga
     7681 tagagagtat gctcattcaa tgtgaactta ctgaatattt ttcctttgtt ataagtggtg
     7741 agtcattaaa acaaagcaaa ccacatcccg aaatatattt aaaagcgaaa cagcaattag
     7801 actgtgactt ctttattgca gtagaggatt caacgattgg tattaaatca gcaaaggcag
     7861 caaatatcta tacggtggca ctaaaacaac caatgcaaat cgatcagagt gaagcagaca
     7921 tacaaattga aagtctacaa gaactggaac aaattgtttt agaaaaaggt tagtgaatta
     7981 atggaaataa ggctatatat ctgtgctagc tattttgact aaccaaaaaa acttgctata
     8041 attcaggtgt agtaaaaggt gctggtaaca ccttttacga aaatgcttcg gcagtgataa
     8101 tgggattggc tttttaagtt ttttattttg ttcgcaatcc ctaaggtcat gtgaatgatc
     8161 ctagggattg ctagaatatt ttgcaacaga accaaaatac gaatagttat caagcagcca
     8221 tattgactgc ggtcaattta ggaggggata ctgatactac tgctgccgta actggtgctc
     8281 ttgcgggtat tctttacggg ggtgaagtga tccctgaaaa ttggagaaaa tcgcttcaaa
     8341 atgaccagct tcttaaaacg atcagtcaga gattttgcga taccgtgcta tctcaaagaa
     8401 tttgaataat ggatatgatg caaatcttgt ttgtactaac gttgtattga caaaaaggat
     8461 tttatttagt acaatgttag tacaaatgat gaacggaggt agaatcatgt ctcaatcaac
     8521 cattacattt aggattcccg acgatgaaaa agaactggta ttagaatatg caaaagcaca
     8581 caattcttca ttgaccgaac tttatcgaaa tgctgtactg gataaaatcg aagatgaaat
     8641 cgatctacgg actttacgga gtgcaattga agtttccaaa gagaaaaaag agagtggtat
     8701 ttctcaagag gaaatggaga aactgctaaa tgaattataa agtcatctac atgccgcaag
     8761 cccagaagca attaaaaaaa atggacagaa accaagctaa aataattgtc gcctggatca
     8821 gaaagaattt agaaaacacg actgacccgc gtcagcacgg aaaaggtttg actggaaata
     8881 agagtgggga atggcgctat cgaattggaa actatcgaat attggctaat atttgtgatt
     8941 ctgaaattga aatccagata ttctcaattg gacatagaag tacaatatat aaaagataac
     9001 cgtttttgga acgaagtaga gtttcgaaag tcaatgtata atcttttgaa ttttgcataa
     9061 taccacaggt agaaactacc cgatactatc ggttggtttc tgcttttt
//
LOCUS       DASHHB010000085         8942 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.089, whole genome shotgun sequence.
ACCESSION   DASHHB010000085 DASHHB010000000
VERSION     DASHHB010000085.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8942)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8942
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.089"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            51..260
                     /locus_tag="U2K71_002553"
     CDS             51..260
                     /locus_tag="U2K71_002553"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316571.1"
                     /translation="MICYISFLILGLPQAVFPEMFSGDWGIVSYSILTLIAMVLGTSF
                     YNAFVSKHVEEYEVLMYLKEKKTSN"
     gene            467..667
                     /locus_tag="U2K71_002554"
                     /pseudo
     CDS             467..667
                     /locus_tag="U2K71_002554"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298761.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1379..1984)
                     /locus_tag="U2K71_002555"
     CDS             complement(1379..1984)
                     /locus_tag="U2K71_002555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7316572.1"
                     /translation="MAKIGYIRVSSQDQNLDRQLAMMEDLNIDKLFQEKASGKDIQRP
                     EFQKLLNYIREGDCVIVASLDRLGRDYEDIKNTVTFMKQKKVALKILDAQFLDFNTGN
                     ELLDTAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAKKAGRYKGRPIEYSENSTDPQ
                     KRLVFKTVVDMLNQGVPVAEIAKENGLSRPTIYKIKKANDL"
     gene            2166..2501
                     /locus_tag="U2K71_002556"
                     /pseudo
     CDS             2166..2501
                     /locus_tag="U2K71_002556"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000343406.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            2666..2893
                     /locus_tag="U2K71_002557"
                     /pseudo
     CDS             2666..2893
                     /locus_tag="U2K71_002557"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613886.1"
                     /note="internal stop; incomplete; partial in the middle of
                     a contig; missing N-terminus and C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
     gene            3019..3990
                     /locus_tag="U2K71_002558"
     CDS             3019..3990
                     /locus_tag="U2K71_002558"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171162.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7316573.1"
                     /translation="MKTTMKDVAALAGVGVGTVSRVINGVKVKEVTKEKVMAAISELN
                     YEPDEYARGMKTNSSNTIALILPTIWHPFFSEFAYYVEEALSQKNYKLFLCNANGSAE
                     KEKEYIQMVKQSKVDGIIGITYSDIDKYVSSNLPFVSIDRHFSEQVSYVTADNYLGGQ
                     IAAQELLTRNCKNLCYVGGVSPYPNETNNRKAGFYNICEENKIAVKVLDMPEPISNLE
                     EQLLAFFSDNPEIDSIFTINDFMALDVIKAQKKLGKEAPKDFQIIGFDGVKLSLQKDY
                     LLSTIVQPIEEMAKVSVETLMAMILNEEPPKRVVVPVFFHAGETTKE"
     gene            4130..5443
                     /locus_tag="U2K71_002559"
     CDS             4130..5443
                     /locus_tag="U2K71_002559"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HEM7316574.1"
                     /translation="MKKRMLAIAAILALTAGGLAGCGSKEKSSGSSDPGEIKIWVQFS
                     DETPEGKAWAEIVNNFNDEYEGEYKAVTEYIPRSGSGGGYEDKVNAAITTNTLPDVIT
                     LDGPNTAAYAHSGVIAPLDDYLKDADMDDVLDSIIQQGTYDGKMYAFGFSESSVGVYY
                     NKSLFKDAGITEDQLPTLEDPWTWTEFQAICQKLTDKYKTPAIDMQMASGDEMLTYAY
                     TPFIWSNGGDVVNADGTKAEDYFNGKKSVEALEFIQELVEKGYTTISPIEKGFETGKY
                     AMMLSGSWTMADLEENYQEIDYGILPYPVSDATGELVSPTGSWQLAMTEKTEKKDASA
                     AFIKFATSTESSRIMSLGNSVLPIRNSTVDLIKDEVSEGMQVLIKQNQLSGHARPVVV
                     AYPQVSRAFQQAVQDISYFEENPDVQKVADSRAAEMQQAIDTSLK"
     gene            5486..6352
                     /locus_tag="U2K71_002560"
     CDS             5486..6352
                     /locus_tag="U2K71_002560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HEM7316575.1"
                     /translation="MKDNLIGYGFLAPALLLLTIFLIIPVIMVIYYAFTDYYLLTPDA
                     RQFIGLENFKRLFADPIFVKSIWNTAKFVVWIIPVQIGAALGMALIINKNRKGNIFFK
                     VAFFAPVVMSLVVISILWLYLLNPNEGLVNTLFAKIGIASQPFLTSPKQAMYTLVFVS
                     AWQGAGYQMLLFLGGMQNIPQDVYEAAEIDGFSKFQQFRYITMPLLKPTAIFILLTTL
                     ISAFKLIIQPMVMTQGGPMNSTMTMVYYIYQTGFTDRMVGYSSSIALIFTTMIGLITI
                     AQRKLVKEDDPA"
     gene            6349..7197
                     /locus_tag="U2K71_002561"
     CDS             6349..7197
                     /locus_tag="U2K71_002561"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HEM7316576.1"
                     /translation="MTKKFGPLMIVEYCLLFLLAALFIFPMLWMVVSSMKPEADVYQN
                     LASFKAFLPSLNPANWFKTYQEVISRFSVGTYLLNSIFYGLTFACGSIIVNSLAGFAF
                     AKVNFSGKKIIFGALLALLIVPMETVLIPQFTIVNSLGLVNTRLAVILPAMASAFNIY
                     LFRNFFIAIPEEIIESAKLDGANIVKIFFHIMLPMSKPAIATVGVLSFISSWNDYIWP
                     LMVLTDSSKFSMQVAITTINTTQPVYINQVMAVLTIATIPLIIVYIVAQKYILQGLGG
                     SGTGIK"
     gene            7211..8683
                     /locus_tag="U2K71_002562"
     CDS             7211..8683
                     /locus_tag="U2K71_002562"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 32 protein"
                     /protein_id="HEM7316577.1"
                     /translation="MLQKLLKANEFIEKNAQTVNQEYRNKFHLMAPIGWMNDPNGFVY
                     YQEEYHLFYQYYPYDSIWGPMHWGHAKSKDLIHWEHLPVALAPGESYDKEGCFSGSAI
                     EKDGKLYLIYTGHNVVEGQVRQVQCLAVSEDGIHFEKYAGNPIIAEDHLASVATTEDF
                     RDPKVFQRKEDYFTVVATKTADARGQIVMFHSKDLLTWEFYSVLLEGTPEQGIVWECP
                     DLFELDGKEVLILSPIQMTPQGNAYQNTNSTVAFIGTVDWQTGKFLVENFHEIDGGLD
                     FYAPQTCENEAGQRIMIAWMQMWQRKIPTNDLGHGWSGSMTLPRELRVADNQLLQRPV
                     SSIYQVIDPQERIEWAKTEENRFLLKDLVYDQHYLRLEINLKQATKLELHYAKGPDHT
                     LLLNYDVERECFTASREHMPYDLSGAEKEVLKKRSVTIPLENNCLTIEIFRDTSAIEL
                     FLNDRLTMSTTFYETEKGQDFLLLADSEVTFSLESGLINL"
     gene            8700..>8942
                     /locus_tag="U2K71_002563"
     CDS             8700..>8942
                     /locus_tag="U2K71_002563"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM7316578.1"
                     /translation="MIYGSIEAGGTKFVCAISDEEMTIKERVSFPTTTPEETIPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         2830 a         1515 c         1831 g         2766 t
ORIGIN      
        1 atttaaaaag aaaagtgaca gataggaata aaaacttatt aaatgtagtt atgatctgtt
       61 atatttcatt tctaatacta ggtcttccac aagcagtgtt tcctgaaatg ttttctggag
      121 actggggtat tgtcagctat tctatattaa cattgattgc tatggtttta ggtacttcat
      181 tttataatgc ttttgtaagt aagcatgttg aggaatatga agtattaatg tacttaaaag
      241 agaaaaaaac aagtaattaa tatgagaaca ccacgcatag tttttatttt gagatattct
      301 agaatgaaac acattaagaa cgaaagcaaa gcaaaagacg ccagaacttc tactaccttc
      361 taagtttttt catatttgtt tgttatgcta tacatagatc tgcataatta gacttgcgct
      421 gcccgttatt gaagtttcag gcagcgctct ttttggggga tcgataatgg aagaagaaaa
      481 aatatttgag aaacgctggc agctagcatc aagtgaacaa agagcaagat acaataattt
      541 gatcacctcc taccccactg ttgactagac atataaggaa aagaaatact tactttggct
      601 gtgccagtta gatatagata cctttgaaac atttgaagtg atcctagata aaattaaaca
      661 gaattgatca gaaacaagcg aagccttctc tcggttcgct tgttttgcta tttattagtt
      721 ggctcagggc aactctaagc tcgtttgaaa gaaaaccgat ggataatccc cttgtttgta
      781 ccactttaga atacatggat gtacgtattg ccattgactc ctattagtta ctgaaaaaaa
      841 atttaaacaa gttgagtatt gcagataaat agctccagtg aggtgaataa ctgctccact
      901 caaggcaata gatagctcca tccagggcaa caaactgctc cactttcata tttaatagat
      961 gaataacctt ctgtataata gaaaggcata cctttcggta tgacaactta tacagaatgt
     1021 tattttattg gttcactatc gaaaaatttt ggagctttat ttttccggta tttctcaacg
     1081 aacgattagt ttaactgtgg gaagctccag acatacgatt cgagaagtta cttctcgagc
     1141 agacaggaag ggggtgactg aactaaccgc tgatatgagc gatacttggc tgtaggaata
     1201 tctttttccg gaaaaacaaa caacagcacg aggttacctt cctgtagact gggaatatgt
     1261 tcataaagaa ctaatgaaaa agaatatgac attgaaactc cttcatagag aatatggggt
     1321 acatggaaca tagaaacgaa aatgcacggt agaaaagcga atgaatatag tataaattct
     1381 acaaatcatt cgctttcttt attttatata tggttggtcg agagagacca ttttctttgg
     1441 caatttctgc aactggaact ccttgattta acatgtccac cacagtttta aagactaatc
     1501 gtttttgcgg atctgttgag ttctcagaat attcaattgg tcgcccctta tatcgaccag
     1561 cttttttggc caataatact ccttgccgct gacgttcacg gattttctca cgttcgtttt
     1621 gtgcaatata acttagcaat gataaaaaca tatcgaacat agcagtatct aggagttcat
     1681 ttccagtatt aaaatctaaa aactgtgcat ctagtatttt taatgctact tttttctgtt
     1741 tcataaaggt aactgtattt tttatgtctt cataatctct gcctaggcga tccagtgatg
     1801 caacaatgac acaatcacct tctcggatat aatttagtaa tttttgaaac tcaggtcgct
     1861 ggatatcttt accactggct ttttcttgga ataatttatc aatgttaagg tcttccatca
     1921 ttgccaattg tcgatctaaa ttttgatctt gtgaagacac tctaatatat ccaatttttg
     1981 ccataaaaac tccttttaaa aaatgatata atttacatgt aaaatttact tgtagtttta
     2041 ctgttatcat agcattttta aaaataaatt ttaagtctac ttataattta ctttggaggt
     2101 tttgaatgaa tgaaattaag cgcaataaaa attcttttgg tgtttattta ggagagccta
     2161 gctcggaaca gttaaataaa ggtgaagctc gtcatgctct atgtcgaaat atattttatg
     2221 gtagaaaagg caaactttat caaacttatt acgacggtat ggaggaacag ctcaacgcat
     2281 taggattagt aactaatgct atcatatatt ggaattcact atatttagaa aaagtctttg
     2341 aacaaattaa aacggaaggg ttcgactgtt ccgaagaagt gttacgcaag caatttccat
     2401 taattagtga tcacattaat tttgttggca aatatagttt ccgctatgaa aaagaattat
     2461 cgaatggaat actacgtccg ttaaatatag agaattattg agaatgttcg tcttttggtt
     2521 cctaccgtgc attttcgcac ggatgttcca gatactattt ttgaatgtat aaaattgaaa
     2581 acacaagaat actaataaat caacgattct tgtgttttta atttttcact accgtgcatt
     2641 ttcgtttcta tgttccatgt accccaatct tgaaaaatta attaaagact tagataaagc
     2701 cattgacgac ttagtcgtgg tgctaccgga gtatcaatgt ttaaccagta tcccaggtat
     2761 tggtcctgtt taggctgctg gaatactcgc tgaaattggt ccaattgaac gtttttctga
     2821 tgaaataaaa ttgacaaagt atgccggcct atattggcga cagagtcaat ccggtaaatc
     2881 tgaatacgaa aattttttta cgaaaagctt cacgtctaat atctatttta aaataaaaat
     2941 attcctgcta atttggaatc gtttccttct tttggtataa taatacctga aattcttttg
     3001 agaggagttg ttcgtgaagt gaaaactact atgaaggatg ttgctgcgtt agcaggagtt
     3061 ggtgtgggta cggtatctag agtaataaat ggagtaaagg tgaaagaggt taccaaagaa
     3121 aaagtaatgg ctgcaattag tgagcttaat tatgagcctg atgaatatgc cagaggtatg
     3181 aaaacgaata gtagcaatac aatagctctt attttaccta cgatttggca tcccttcttt
     3241 tccgagtttg cctactatgt agaggaagct ctcagccaaa aaaattataa gctttttttg
     3301 tgtaatgcga acggcagtgc ggaaaaagaa aaagaatata tccaaatggt caagcagagt
     3361 aaagtagacg gaattatagg tattacctat tcagacattg ataaatatgt atcatcaaat
     3421 ttaccatttg tcagtataga ccgccatttt tcggaacagg tttcttatgt aacggcagat
     3481 aattatttag gtgggcaaat tgccgctcaa gaactactta ctcgtaactg taaaaacttg
     3541 tgctatgttg ggggtgtttc accttaccct aacgaaacaa acaatcgtaa agctggtttt
     3601 tacaatattt gtgaggaaaa taaaatagct gtgaaggtat tggatatgcc tgaaccaatt
     3661 agcaatttag aggaacaatt gctcgctttt ttttcggata atcctgaaat tgatagcatt
     3721 tttaccatca atgatttcat ggctttggat gtgataaaag cccaaaaaaa actagggaag
     3781 gaagcaccta aggattttca aattattggt tttgatggag tcaagctatc gttgcaaaaa
     3841 gactatttac tatcaacgat tgtacagccg atagaggaaa tggccaaggt ttcagtagaa
     3901 acactcatgg cgatgatttt aaatgaagaa ccgcctaagc gtgtggtagt accggttttc
     3961 tttcacgctg gagagacgac taaagagtaa aaaaccggtt tataacgatt tttttgagat
     4021 aggttgacag aaagtgattt caatgatatc ttaaaaccaa ttaattggaa acgttaccaa
     4081 ttaattgaaa aataaaacgg aaacgtttca aaaaatagga ggaacatcga tgaaaaagag
     4141 aatgttagca attgcagcaa ttttagcatt aacagctgga ggacttgcag gatgtggcag
     4201 taaggagaaa agttcaggta gcagtgatcc aggagaaatt aagatttggg ttcagttttc
     4261 tgatgaaacg cctgaaggaa aagcttgggc agagattgtg aacaatttta atgatgaata
     4321 cgagggtgaa tataaagctg ttactgagta tattcctcgt agcggaagcg gcggtggata
     4381 tgaggataaa gtaaatgctg caattacaac caataccctt cctgatgtta ttacgttaga
     4441 tggaccgaat actgcagctt atgctcattc aggagtcatt gctccattag atgattattt
     4501 gaaagatgcg gacatggatg atgtattaga cagcattatt cagcaaggaa cctacgatgg
     4561 caaaatgtat gcgtttggtt tctcagagtc cagtgtgggt gtctattaca ataagtcgct
     4621 gttcaaagat gctggaatta cagaagatca attgccgacg ttagaagatc cttggacttg
     4681 gacggaattt caagcaattt gccaaaagct gactgataaa tacaaaacgc ctgcaattga
     4741 tatgcaaatg gcgtcgggag acgaaatgct gacctacgca tatacaccat ttatatggtc
     4801 gaatggcggc gatgtcgtta atgctgatgg aaccaaagcc gaagactatt ttaacggtaa
     4861 aaaatccgtt gaagcgttgg aatttattca agaattggtt gaaaaaggtt acaccacaat
     4921 cagtcctatt gaaaaaggat ttgaaactgg caaatatgcc atgatgctga gcggttcttg
     4981 gacaatggca gatttagagg aaaactatca agagattgat tatggtattt tgccttaccc
     5041 cgtttcagat gcaactgggg agttagtttc accaaccgga agctggcagt tagcaatgac
     5101 tgagaaaaca gagaaaaagg atgcttctgc ggcctttatt aagtttgcaa ccagtacaga
     5161 atctagtcgt attatgagtt tagggaacag tgttctacct atcagaaatt caacagttga
     5221 tttaattaaa gatgaagtgt cggaaggaat gcaggtatta attaagcaaa accaactttc
     5281 tggacatgcc cgcccagtgg ttgtagctta tccgcaagta tctagagcat tccaacaagc
     5341 agttcaagat attagctact tcgaggaaaa cccagatgta caaaaagtag cagatagtcg
     5401 cgctgctgaa atgcaacagg caattgatac ttcactaaaa tagaagtttc taaagggggc
     5461 caatcaagtg ttaaaaaaac cgacaatgaa agacaatttg ataggttacg gctttctagc
     5521 gccagctttg ctgctgctga cgatttttct gatcataccc gtaatcatgg taatttatta
     5581 tgcctttact gattattatt tgttaacgcc agatgctaga caatttatcg gcttagaaaa
     5641 ttttaagcga ctgttcgctg atccaatttt tgtaaaaagt atttggaaca cggctaaatt
     5701 tgttgtttgg attattccgg tccaaatagg ggcagcttta ggaatggcac ttattatcaa
     5761 caaaaatcgt aaaggaaata tcttttttaa agtagccttt tttgcgccag tcgtgatgtc
     5821 cttagtcgtt atttcgattt tatggttgta cttgttaaat cctaatgaag gtctggttaa
     5881 tacgctattt gcaaagattg gaattgcttc gcagcctttt ttgactagtc ccaaacaagc
     5941 aatgtatacg cttgttttcg tatcggcatg gcaaggtgca ggatatcaga tgctgctgtt
     6001 tttaggggga atgcaaaata ttccgcaaga cgtttatgag gcagccgaaa ttgatggttt
     6061 ttctaagttt cagcaatttc gctatatcac catgccatta ttaaagccca cggcgatttt
     6121 tattttactt accacattga tttcggcctt taaactaatc attcagccaa tggtaatgac
     6181 tcaaggtgga ccaatgaatt ctactatgac aatggtttat tacatttacc aaactggatt
     6241 tacagaccgt atggtaggct actccagctc cattgcgtta atctttacca ctatgattgg
     6301 tttaattaca attgctcaaa gaaaattggt aaaggaggat gatccagcat gaccaagaaa
     6361 tttggaccgc taatgatcgt agaatattgt ttgctgttcc ttttagcggc attattcatt
     6421 ttcccgatgc tgtggatggt agtttcctct atgaagcctg aagcagatgt ctatcaaaat
     6481 ttagcttcct tcaaagcttt tttacctagt ttgaatccag ctaattggtt taaaacttat
     6541 caagaggtta tttcccggtt cagtgtggga acttatttat taaacagtat tttttatggt
     6601 ttaacctttg cctgtgggtc aatcattgta aactctttgg cgggttttgc ctttgcgaag
     6661 gttaattttt caggcaagaa aattatcttt ggagctttgt tagctctgct gattgtcccg
     6721 atggagacgg ttttaattcc gcaatttacc atcgttaatt cactaggctt agtgaatact
     6781 cgcttggcag tgattttacc agcgatggcc agcgcgttta atatctatct gtttagaaat
     6841 ttctttatcg cgattccaga agaaatcatt gaatcagcga aattagatgg tgcaaatatt
     6901 gttaaaattt tctttcacat catgctgcca atgtctaaac cagccatagc cacagtaggt
     6961 gtcttatcct ttatctccag ctggaatgat tatatttggc cgttaatggt cttaactgac
     7021 agcagcaaat tttcaatgca agtggcaatt actacgatca atactacaca gccagtttat
     7081 atcaatcaag tgatggctgt tttaaccatt gcaacgattc cactgattat tgtttatatc
     7141 gttgcacaaa aatatattct gcagggactt ggcggttctg gaacgggtat aaaataggag
     7201 tgaatagtga atgttgcaaa aacttttaaa ggccaatgag tttattgaga aaaatgctca
     7261 aacagtcaat caagaatacc ggaataaatt tcatttgatg gctccgattg gctggatgaa
     7321 tgatccaaat ggttttgttt attatcaaga ggagtatcac ttgttttatc aatattatcc
     7381 ttacgacagc atctggggac caatgcattg gggccatgct aaaagcaagg atctaattca
     7441 ctgggagcat ttgccagtag cactggcacc aggtgaaagc tatgacaagg aaggctgttt
     7501 ttccggcagt gccatagaaa aggatggcaa gctgtatttg atttatacag gacacaatgt
     7561 tgtggaaggg caagttcgac aagttcagtg tttggcagtt tcagaagatg gcattcactt
     7621 tgaaaaatat gccggaaatc caatcatcgc agaagatcac ttagcttctg ttgcaacgac
     7681 cgaagatttc cgcgatccca aagtgtttca gcgaaaagaa gactatttta ctgtagtcgc
     7741 tactaaaact gcagatgctc gtggacaaat tgtgatgttc cattccaaag atttactaac
     7801 ttgggagttt tattccgtat tgctggaggg aactccagaa caaggcattg tatgggaatg
     7861 cccagacttg tttgaactag atggcaaaga agtcttgatt ctttctccta ttcaaatgac
     7921 tccacaaggc aatgcttacc aaaatacgaa ctcaactgtt gctttcatcg gtactgtcga
     7981 ttggcaaaca ggcaaatttc tagtagagaa ttttcatgaa atcgatggcg gtttagattt
     8041 ttatgcaccc cagacctgtg aaaacgaagc aggccaaaga ataatgattg cttggatgca
     8101 aatgtggcag cgtaaaatac ctacgaatga tctgggccat ggttggtcag gttccatgac
     8161 cttgcctaga gagctgaggg tggctgataa tcaattgttg caacggccag tttcatcaat
     8221 ttatcaagta attgatccgc aggaaaggat tgaatgggcg aagactgaag agaatcgctt
     8281 cttactgaag gacttagtat atgaccagca ctatttacgc ttggaaatca atttaaaaca
     8341 ggcaacgaaa ctagaacttc actatgcgaa gggacccgac catacattac tgcttaacta
     8401 tgatgttgaa cgggagtgct ttactgcttc cagggaacac atgccgtatg atttatcagg
     8461 agctgaaaag gaagtgttga aaaagcgcag tgtgacgatt cctttggaaa ataattgtct
     8521 aaccatcgaa attttccgtg atacttcagc tattgagctt tttctaaatg atcgtctgac
     8581 tatgagtaca actttttatg agacagaaaa aggacaggat tttcttttgc tggcagacag
     8641 tgaagtaacc ttttcattgg aaagcggctt aattaatcta tagaagggag agctcagtga
     8701 tgatttatgg ttcaatcgaa gcaggcggca caaaatttgt gtgtgccatt agcgatgaag
     8761 aaatgacgat caaggaaaga gtgagctttc cgacaacgac acctgaggaa acaatacctt
     8821 tagtcattga cttcttcaaa caataccaag cagatttagc agggattggg attggctcat
     8881 ttgggcctat cgatatccat cgcgattccg ccacttatgg gtacattacc tcgaccccga
     8941 aa
//
LOCUS       DASHHB010000086         8864 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.090, whole genome shotgun sequence.
ACCESSION   DASHHB010000086 DASHHB010000000
VERSION     DASHHB010000086.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8864)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8864)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8864
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.090"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            91..1425
                     /gene="dnaA"
                     /locus_tag="U2K71_002564"
     CDS             91..1425
                     /gene="dnaA"
                     /locus_tag="U2K71_002564"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010720504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HEM7316579.1"
                     /translation="MVSLDALWNELKATYQKDLSPASYNTWIETANPRTLDQNQLVVE
                     VPSKIHKEYWEKNLATKIVEVGYMLSGNEIIPRFITGEEAEQEEVIEEKNPKVVAPSP
                     LKKAMLNPKYTFDTFVIGKGNQMAHAAALVVAEDPGSIYNPLFFYGGVGLGKTHLMHA
                     IGHQMLQSQPNAKVKYVSSETFANDFINSIQNKTAEEFRQEYRNVDLLLVDDIQFFAE
                     KEATQEEFFHTFNALYNEGKQIVLTSDRLPNEIPKLQERLVSRFAWGLSVDITPPDLE
                     TRTAILRKKAAAERLEIPDDTLSYIAGQIDSNIRELEGALVRVQAFAAMNSEDISTSL
                     AADALKTLKSGKGHPQLSILQIQEEVAKYYHIQLKDIKGKKRVKSIVVPRQIAMYLAR
                     ELTDNSLPKIGAEFGGKDHTTVIHAHEKIQQLMDSSVSMQNEVSEIKNFLLN"
     gene            1623..2753
                     /gene="dnaN"
                     /locus_tag="U2K71_002565"
     CDS             1623..2753
                     /gene="dnaN"
                     /locus_tag="U2K71_002565"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HEM7316580.1"
                     /translation="MKVTLNRASFMQELQTVQRAISSKTTIPILTGVKITLTQEGLTL
                     TGSNADISIETFLSVENEKANMQIESTGSIVLQARFFSEIIRRLPEETFTLEVLENKQ
                     VAITSGKANFIVNGLDADNYPHLPVVESHNQMKLPVHVLTKLINETVFAVSQHESRPI
                     LTGVHFILSDNSLLAVATDSHRLSQRVIPVEQAADHFDIVIPGKSLIELSRSLTNEEE
                     IVEISIMENQVLFKTETMYFYSRLLEGNYPDTNRLIPSSFNTEVEFSVPSFLAAIERA
                     SLLSHEGRNNIVRLSIRPDAVVLYGNSPEIGKVEESLSYTASSGDPLDISFNPDYMKA
                     ALRAFGDMSIKVKFISAIRPFTLEPTEDGVQFIQLITPVRTN"
     gene            2982..3224
                     /gene="yaaA"
                     /locus_tag="U2K71_002566"
     CDS             2982..3224
                     /gene="yaaA"
                     /locus_tag="U2K71_002566"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S4 domain-containing protein YaaA"
                     /protein_id="HEM7316581.1"
                     /translation="MKQQVILNTEFMTLGQMLKEVTVIGSGGQAKWYLAENTVLVDGE
                     PENRRGRKLYPGMMVEVPEVGTFFMVKKGNDDNEAE"
     gene            3211..4335
                     /gene="recF"
                     /locus_tag="U2K71_002567"
     CDS             3211..4335
                     /gene="recF"
                     /locus_tag="U2K71_002567"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA replication/repair protein RecF"
                     /protein_id="HEM7316582.1"
                     /translation="MRLNKLYLKNYRNYEELNIEFSKNLVIFLGENAQGKTNLLESIY
                     VLAMTRSHRTSSEQELIGWTDDQAMIQGEITKGSSTLPLEILLSKKGRKTKINHIEQK
                     KLSTYVGQMNVILFAPEDLSLVKGSPQIRRKFLDMELGQINPIYLYDLVQYQAILKQR
                     NQYLKQLNEKKQTDLLYLDILSEQLAAFGSKVLKARAQFVKRLEFWANSLHQQITHQK
                     EQLEIEYLTAVDSLETHTQEQIQEQFLALLNQNKKKDLFRGTTTVGPHRDDLSFFINQ
                     KNVQTYGSQGQQRTTALSVKLAEIDLIKEETGEYPILLLDDVMSELDDSRQLHLLETI
                     EGKVQTFLTTTTLDHVKNKMTVEPEIFYVQQGKLKGEETE"
     gene            4332..6278
                     /gene="gyrB"
                     /locus_tag="U2K71_002568"
     CDS             4332..6278
                     /gene="gyrB"
                     /locus_tag="U2K71_002568"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003129249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase (ATP-hydrolyzing) subunit B"
                     /protein_id="HEM7316583.1"
                     /translation="MTEERSLVERAKEYDASQIQVLEGLEAVRKRPGMYIGSTSSEGL
                     HHLVWEIVDNSIDEVLAGFATKIHVIIEKDNSITVIDDGRGIPVDIQAKTGRPAVETV
                     FTVLHAGGKFGGGGYKVSGGLHGVGSSVVNALSTQLDVKVYKEGNVYYQEYRRGAVVD
                     DLKIIEQTDRHGTTVHFTPDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKRE
                     EEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNI
                     MSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVIS
                     IKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRAR
                     LAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREF
                     QAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDA
                     DVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELK
                     QVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEML
                     MGDRVEPRRAFIEENAHYVKNLDI"
     gene            6298..8769
                     /gene="gyrA"
                     /locus_tag="U2K71_002569"
     CDS             6298..8769
                     /gene="gyrA"
                     /locus_tag="U2K71_002569"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631904.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HEM7316584.1"
                     /translation="MSEEIRENIHDVNLTSEMKDSFIDYAMSVIVARALPDVRDGLKP
                     VHRRILYGMNELGVTPDKAHKKSARIVGDVMGKYHPHGDYAIYESMVRMAQPFSYRYM
                     LVDGHGNFGSVDGDGAAAMRYTEARMSKIATEMLRDINKNTVDFQSNYDDTEKEPVVL
                     PARFPNLLVNGTTGIAVGMATNIPPHNLSEVVAAIDLLMDNPDVTTNELMEVLPGPDF
                     PTGGLVMGKSGIRRAYETGKGSITVRAKVEITEMPNGKERIIVTELPYMVNKAKLIER
                     ISELHRDKRIEGITDLRDESSREGMRIVIDIRRDASASVILNNLYKLTSLQNSFGFNM
                     LAIEKGVPKVLSLKQILENYIEHQREVITRRTVFEKEKAEARAHILEGLRIALDHIDE
                     IIAIIRGSKSDDEAKATMIERFDLSDRQSQAILDMRLRRLTGLEREKIENEYQELLKL
                     IEDLTDILARPERVTEIIKNELADLNQRFGDKRRTELLVGEVLSLEDEDLIEEEEIVI
                     TLTNNGYIKRLANNEFRAQRRGGRGVQGMGIHNDDFVKNLVSCSTHDTLLFFTNNGKV
                     YRAKGYEIPEYGRTAKGIPIINLLGIDSSESIQAIIAVTGEAEEGHYLFFTTRQGTVK
                     RTSVTAFANIRSNGLIAIGLKEDDELVNVLLTDGQSNIIIGTHNGYSVTFKEEAVRDM
                     GRTASGVRGIRLREEDYVVGAALMKEDQEVLVITEKGYGKRTKVSEYPVKGRGGKGIK
                     TANITEKNGPLAGLTTVSGDEDIMLITDKGVIIRFNVSTVSQTGRSTLGVRLMKMEAD
                     TKVVTMAAVEPEAAEQTVEEQGSEE"
BASE COUNT         2954 a         1606 c         1923 g         2381 t
ORIGIN      
        1 atttttttct tcggtttctg tggaaaacta ttgaaaaaaa tgctaaatta gtactagtta
       61 ttcacttttt aatgaaggag gaggccttat atggtatccc tcgatgcttt atggaatgaa
      121 ttaaaagcaa cataccaaaa ggatttatcg cctgctagct ataatacatg gatcgaaaca
      181 gcgaaccctc gaacccttga tcaaaaccag ctcgttgttg aggttcctag caagattcat
      241 aaagaatatt gggaaaaaaa cctggcgaca aaaatcgttg aagtcggtta tatgttatca
      301 ggaaatgaga ttatccctcg ctttattact ggcgaagaag cagagcaaga agaagttata
      361 gaagaaaaaa atccaaaagt tgtggcacct agtccgctaa aaaaagccat gctgaaccct
      421 aaatatacct ttgatacgtt tgtcattggt aaaggaaacc agatggctca tgcagcagcg
      481 ctcgttgtag cagaagatcc aggttctatt tataacccgc tcttctttta tggaggcgtg
      541 ggacttggta agactcactt gatgcatgca attggtcatc aaatgctgca aagtcagcct
      601 aatgccaaag taaaatatgt aagcagtgaa acttttgcta atgatttcat caattcgatc
      661 caaaacaaaa cagcagaaga gttccgtcaa gaatatcgaa atgttgattt actgctagtg
      721 gatgacattc aattttttgc agaaaaagaa gctacgcaag aagagttttt ccatactttc
      781 aatgccttgt acaatgaagg gaaacaaatc gtactaacca gtgatcggtt gccaaacgag
      841 attccgaaac tgcaagaacg cctcgtttct cgctttgcat gggggctgtc tgttgatatt
      901 acaccgccgg atcttgaaac aagaacagcg attttacgta aaaaagcggc ggctgaacgt
      961 ctagagatcc cagatgacac actaagctac atcgcaggac aaattgattc taacattcgt
     1021 gaattagaag gtgcgcttgt tcgagtgcaa gcttttgctg cgatgaatag tgaagatatt
     1081 tcgactagtc tagctgcaga cgcactgaaa acgttaaaat caggaaaagg acatcctcaa
     1141 ttgtcaatcc tgcagatcca agaagaagtg gcaaagtatt atcacatcca attgaaagat
     1201 atcaaaggaa agaaacgcgt caaatctatt gttgttccta gacaaatcgc tatgtatttg
     1261 gcacgtgagc tcacagataa ttctttaccg aaaatcggag ctgaattcgg tggcaaggat
     1321 catacaacgg ttatccatgc tcatgaaaaa atccagcagc tgatggattc tagtgtcagt
     1381 atgcaaaatg aagtatcaga aatcaaaaac tttttactaa attaatttgt cgattctatc
     1441 aactgaaaat agcatgtgga taaaagaaaa aaacgttcaa aagttatgca cactttttcc
     1501 acagctgttt tttcgtttct ctcttacgtt ttttgagttt tccacagaat caacatgccc
     1561 tattactttt attattcttt ttaactaata ataaataaac aaacattcaa aggagataca
     1621 tcatgaaagt tactttaaac cgagctagct ttatgcagga attgcaaact gttcaacgag
     1681 ctatttcaag caaaaccacg atccctattt tgacaggtgt aaaaatcaca ctgacacaag
     1741 aaggtttgac tttgacgggg agcaacgctg atatatcaat tgaaactttt ttgtctgttg
     1801 aaaacgaaaa agcaaatatg caaatcgaat ctactggttc cattgtttta caagcacgtt
     1861 tctttagcga aatcattcgg agacttcctg aagaaacatt tactttagaa gttttagaaa
     1921 ataaacaagt agcgattact tctggaaaag cgaattttat cgtaaatgga ttagatgcag
     1981 ataactatcc tcatcttcct gttgtcgaaa gccataacca gatgaaatta cctgtacacg
     2041 tattgactaa actaatcaac gaaacagttt ttgctgtctc acaacatgag agtcgtccaa
     2101 tcttgacagg tgtgcatttc attttatctg ataattcttt attggctgta gctactgatt
     2161 ctcaccgtct aagtcaacgc gtgattccag tagaacaagc ggctgatcat tttgatattg
     2221 ttattcctgg aaaaagtttg atcgaattat ctcgctcatt aacaaatgaa gaagaaatcg
     2281 tcgaaatcag cattatggaa aaccaagtgt tgttcaaaac agaaacgatg tatttctatt
     2341 cccgtttgct agaaggaaac tatcctgata ccaatcgttt gattccttca agttttaata
     2401 cagaggttga attttctgtt cctagctttt tagcggcgat cgaacgggct tctttacttt
     2461 ctcatgaagg ccgtaacaac atcgttcgtt tatctattcg tccagatgcg gttgttttat
     2521 acggaaattc gccagaaatc gggaaagtag aagaaagctt gagttataca gcaagcagtg
     2581 gcgatccatt agatatttct ttcaatccgg attatatgaa agcagcgttg cgtgcatttg
     2641 gagatatgag catcaaagtg aaatttatct ctgctattcg tccatttaca ttggaaccaa
     2701 cggaagatgg cgttcaattt attcagctga ttactcctgt acgtacaaat taacaatgat
     2761 tttcattgtt cttgaaaatt taattgaaaa gaaatagaaa aaataaaatt ttaaacagaa
     2821 ggccctaaaa tctcttttaa ggctttctgt ttatttttgt ttaaatcacc ttttttagat
     2881 taaatcgtct tatatggaaa aatatatgga taaatttgtt ttattgcgaa aaaaaaggta
     2941 taatagagta tacttttaat aatgaaaatg agggatgggc attgaaacaa caagtcattc
     3001 taaatacgga attcatgacg ttgggccaaa tgttgaaaga agtcacagtt atcggtagtg
     3061 gcggacaagc aaaatggtat ttggcagaaa acactgtgtt agtcgatggc gaaccagaaa
     3121 atcgtcgtgg acgcaaactt tatcccggca tgatggttga agttccagaa gtaggaactt
     3181 tttttatggt aaaaaaagga aacgacgata atgaggctga ataagctgta tttaaaaaat
     3241 tatcgaaatt acgaagagct gaacatcgaa ttttccaaga acctcgtcat ctttttagga
     3301 gaaaatgcgc aaggaaaaac gaatcttttg gaaagtatct acgtattagc tatgacgcga
     3361 agtcatcgga caagtagtga gcaggaattg attggctgga ctgacgatca ggcgatgatt
     3421 caaggagaga tcacaaaagg ttcatccact cttccattag aaatcttatt atctaaaaaa
     3481 ggccgaaaaa ccaagatcaa ccatatcgaa caaaaaaagt taagtactta cgtgggacaa
     3541 atgaatgtca ttttatttgc accagaagat ctttctttag taaaaggcag cccgcagatc
     3601 aggcggaagt ttttagatat ggaattaggc cagatcaatc caatctatct ttatgatctt
     3661 gtccaatacc aagcaatttt gaaacaaaga aaccagtatt taaagcaatt gaatgaaaaa
     3721 aaacaaactg atttgcttta tttggatatt ttgtccgaac aactagcagc gtttggcagc
     3781 aaagtattga aagcaagagc acagttcgtc aagcgattgg agttttgggc taattctttg
     3841 catcagcaaa tcacccatca aaaagagcag ctggaaatcg agtatctcac cgcagtcgat
     3901 tctttagaaa cacatacgca agagcaaatc caagaacaat ttctcgcact tttgaaccaa
     3961 aataaaaaga aagacctttt tcggggaaca acgacagttg gtcctcatcg tgacgatctt
     4021 tcctttttta ttaatcagaa aaatgtgcag acttatggat cgcaaggaca gcaaagaaca
     4081 actgcactaa gtgtcaaatt agcagaaatc gatttgatta aagaagagac gggggaatat
     4141 ccaatccttt tgttagatga tgtgatgagc gagctggatg atagtcgtca actccatctg
     4201 ctagaaacaa tcgaaggaaa agttcaaacg ttcttaacaa caacgacact tgaccatgta
     4261 aaaaataaaa tgactgttga acctgagatt ttttatgttc aacaagggaa actaaaagga
     4321 gaagaaactg aatgacagaa gaaagaagtt tagtagaacg cgctaaagag tatgatgcca
     4381 gtcagattca agtacttgaa ggacttgaag ccgttcgtaa acgacctggc atgtatatcg
     4441 gttcgactag ttctgagggt ctgcatcacc ttgtttggga aattgtcgat aattcgatcg
     4501 atgaagtatt agcaggtttt gcaacgaaaa tccatgtgat tattgaaaaa gacaacagta
     4561 tcaccgttat tgacgacggt cgggggatac ctgtagatat ccaggcaaaa actggacgtc
     4621 ctgcagttga aacggtcttt actgttttac atgctggagg aaaattcggc ggtggtgggt
     4681 acaaagtttc tggtggactt cacggagtag gatcttctgt tgttaacgcg ctttctacac
     4741 agcttgatgt caaagtatac aaagaaggaa atgtgtacta tcaagaatat cgtcgtggag
     4801 ctgtagtgga cgacttaaag attatcgaac aaacggatcg ccatggaaca acggttcact
     4861 ttacgccaga ccctgaaatt tttacagaga caactgagtt tgatttttca aaattggcta
     4921 cccggatccg tgaacttgct ttcttgaacc gcgggatgcg tatatcgatt gaagacaaac
     4981 gagaagaaga accagtcatc aatgaatacc attatgacgg cgggattaaa agttatgtcg
     5041 aatacttgaa tgcaaacaaa accgtgattt ttccagaacc tgtatatttg gaaggtgaac
     5101 agcaagatat cgcagttgaa gtctctatgc aatatacaga cggctaccat tccaatatca
     5161 tgagttttgc caacaatatc catacgtatg aaggcggaac acatgaatca ggattcaaga
     5221 ctgctttaac tcgtgtgata aatgattatg cccgaaaaca aaagctgatg aaagaaaatg
     5281 acgacaactt gaccggtgaa gacgtgcgag aaggattgac agctgttatt tctatcaagc
     5341 atcctgaccc tcaattcgaa ggacaaacaa aaacaaaatt gggcaattcc gaagtacgga
     5401 cagtcactga tcggttattt agcgaacatt tcatgaaatt cttgctggaa aacccaagtg
     5461 tgggccgtca aattgttgaa aaaggattac tagcttccag agctcgtcta gcagcgaaac
     5521 gtgcgcgtga agtaacgaga cgtaaaggtg ctttagagat tagtaatctg ccaggaaaat
     5581 tagcggactg ctctagcaac gatcctgaaa aatgcgaatt gttcatcgtc gaaggagatt
     5641 cggccggcgg atcagctaag caaggtcgta atcgagagtt tcaagccatc ttgcctattc
     5701 gtgggaaaat cttgaatgtt gaaaaagcaa gcatggacaa gatcttggca aatgaagaga
     5761 tccggtcttt atttactgct atgggtacag ggtttggaga agattttgat gtatcgaaag
     5821 ctcgctacca caaattagtt atcatgactg atgccgatgt cgacggcgcg catatccgta
     5881 cattgctgct gactcttttt tatcgttaca tgcgtcctat tgttgaagct ggatacgtct
     5941 atattgctca gccaccttta tacggggtaa aacaagggaa gaatattacg tatgtacaac
     6001 caggaaaaaa tgcagaagaa gagttgaaac aagtcgttgc ttcacttcct gcctcaccaa
     6061 aaccaagcgt tcagcgttat aaaggacttg gagaaatgga tgatcatcaa ctatgggaaa
     6121 caactatgga cccaagcaat cggatgatgg cccgcgtcag tgtggacgat gctattgcag
     6181 cagatcagat atttgaaatg ttgatggggg atcgagtaga accccgtcgt gcattcatcg
     6241 aggaaaatgc acactatgtg aagaacttag atatttaatc atgaaggagg aaaaagaatg
     6301 agtgaagaaa tcagagaaaa catccatgat gtcaatctaa ccagcgaaat gaaagactcc
     6361 ttcatcgatt atgcgatgag tgttattgtc gcacgtgcac ttcctgatgt cagagatggt
     6421 ctgaaacccg ttcatcgccg tattttatac ggcatgaacg aattaggtgt aacaccggat
     6481 aaagcccata aaaaatctgc cagaatcgtc ggagatgtca tgggtaaata ccatcctcat
     6541 ggagactatg cgatctatga gtcaatggta cggatggcac agccattcag ctaccgttat
     6601 atgctagtgg atggtcatgg aaactttggt tctgttgacg gtgacggtgc cgccgctatg
     6661 cgttataccg aagcacggat gagcaaaatc gctacagaaa tgctgcgtga tatcaataag
     6721 aacactgttg atttccaaag caactatgat gatacagaaa aagaaccagt tgtactacct
     6781 gctcgcttcc cgaatctttt agtgaacgga acaacgggga tcgccgtagg tatggccacg
     6841 aatatcccac ctcataactt gtctgaggta gtagcggcta tcgatttgct gatggataat
     6901 cctgatgtga cgaccaatga attgatggaa gtattacctg gaccagattt tccaacaggt
     6961 ggtttggtta tgggaaaatc aggaatccga agagcgtatg aaacaggtaa gggttctatt
     7021 accgtacgtg caaaagttga gattactgag atgccaaatg gtaaagaacg gatcatcgtg
     7081 acagaattgc cttatatggt caataaagca aaattgatcg aacggatttc tgaactgcac
     7141 agagacaagc gtatcgaagg gatcacagat ttgcgggacg aatcttcccg tgaaggtatg
     7201 cggatcgtca ttgacatccg tcgtgacgcc agcgcatctg ttatcttgaa taacttatat
     7261 aaattgacat ccttgcaaaa ttcatttgga ttcaatatgt tggcgatcga aaaaggtgtg
     7321 ccgaaagttt tgagcttgaa acaaattctt gaaaactata tcgaacatca gcgcgaagtc
     7381 atcacaagac ggacagtctt tgaaaaagaa aaagcagaag caagggcaca cattttagaa
     7441 ggtttgcgta ttgctttgga tcatatcgat gaaatcattg cgattatccg cgggtctaaa
     7501 tcagatgacg aagcaaaagc gacgatgatc gaacggttcg atttatccga tcgccagtct
     7561 caagctattt tagatatgcg tttacgccga ttgaccggtt tggaacggga aaaaatcgaa
     7621 aatgaatacc aagaactctt aaaactgatt gaagatttaa ctgacattct ggcaagacca
     7681 gaacgtgtta cagaaatcat caagaatgaa ttagcagact tgaatcaacg atttggtgat
     7741 aaacgacgca cagaattact agtcggtgag gtcttaagtc tggaagatga agatttaatc
     7801 gaagaagaag aaatcgttat tacattgaca aacaatggtt acatcaaacg tttggcaaat
     7861 aatgaattca gagctcaacg aaggggtgga cgtggtgtcc aaggaatggg tatccacaat
     7921 gacgacttcg tcaaaaatct ggtttcttgt tctacccatg atacattgtt attctttacg
     7981 aataacggaa aagtgtatcg ggcaaaagga tacgaaattc cagaatacgg gcgcacagcc
     8041 aaaggaatac caattatcaa tttactaggt atcgattcga gtgaaagcat ccaggcaatc
     8101 attgcggtga cgggagaagc agaagaaggc cactatctct tctttacgac gcgtcaaggt
     8161 acagtaaaac gtacttctgt cactgctttt gctaacatcc gaagtaatgg tctgatcgca
     8221 attggtttga aagaagacga tgagctcgtc aatgtgctac tgacagatgg tcaatctaat
     8281 atcattattg gaacacataa cggctactct gtcactttca aagaagaagc agtccgcgat
     8341 atgggacgta cagcttccgg ggttagagga atacgcctgc gtgaggaaga ttatgtcgtt
     8401 ggggcagcct taatgaagga agatcaagaa gtattagtga tcacagaaaa aggttacgga
     8461 aaacggacaa aagtatcgga atatccagtg aaaggccgcg gaggtaaagg gatcaaaacg
     8521 gcgaacatca ctgaaaaaaa tggtccacta gctggattga ccactgtttc tggagatgaa
     8581 gacatcatgc tgattacaga taaaggtgtc attatccgtt tcaacgtatc gactgtttct
     8641 caaactggac gttcaacatt aggcgtacgt ttgatgaaga tggaagctga tacgaaagtt
     8701 gtcacgatgg cagcagtcga accagaagct gcagaacaaa cagttgaaga acaaggatca
     8761 gaagaatagc catcaagact aattgtgtaa gctagcaaga atgaccgatt aaaaaatctg
     8821 gttattccgg ctctttgtca aatgatgttg gtaaagagaa atct
//
LOCUS       DASHHB010000087         8490 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.091, whole genome shotgun sequence.
ACCESSION   DASHHB010000087 DASHHB010000000
VERSION     DASHHB010000087.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8490)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8490)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8490
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.091"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..173)
                     /locus_tag="U2K71_002570"
     CDS             complement(<1..173)
                     /locus_tag="U2K71_002570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006735101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7316585.1"
                     /translation="MATKIADELENHVLETGMQKITTHASITAKPFFEKRGYKVINEQ
                     TVELRGQLFTNFL"
     gene            complement(593..1714)
                     /locus_tag="U2K71_002571"
     CDS             complement(593..1714)
                     /locus_tag="U2K71_002571"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010730282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS4 family transposase"
                     /protein_id="HEM7316586.1"
                     /translation="MDKYNVKTSFQKWFSAINFSGLSENSQSLISDFDFYSKKLDFQT
                     TLKVLLHAVYEELPSYREIDRAFLDQRLCKELGIDSLCYSSLSRRAPEIKQEVLMEIF
                     TQLVARISAQQPSSKTTSLQLIDSTTIPLNKAWFPWAKFRKTKSGIKLHLNLCYLDKT
                     NQYPESFTMTNASEHDRNHLEVLVDKTQATYVVDRGYFDYKLLDKLNRDGYFFVTRTK
                     SNTKITILDQIEVADTTTRDGTIISDQQVILVGGVNHVTERFRLVTVLTKGQKILRMV
                     TNLFDVSPNEVADMYQARWQIELLFKHLKQNLTIKRLYSHSEQGAINQVILTLIATLL
                     TYVIKIELNTTATLFQLKRTFHYLMFEPAEEWFKRYRPG"
     gene            2042..3451
                     /gene="istA"
                     /locus_tag="U2K71_002572"
     CDS             2042..3451
                     /gene="istA"
                     /locus_tag="U2K71_002572"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21 family transposase"
                     /protein_id="HEM7316587.1"
                     /translation="MDDIKTIKHLRNNRDQSIDQIRKAMNINWRTAKKYADSDQLFIT
                     DPTRKNKSMMDDGWGEIIGSWLEEDLRLRRKLRRTAKAIHEQLKPLGFPGSYRTVCVY
                     ISQWKKQRYDETNYQAVDRLDHPIGEAQVDFGTMEVMHEDHMKDIQLLIMSFPYSNAT
                     SASAMPAQNQECFLEALKQLFNQCGGVPKTIRIDNLTPAVKKTKSKFEEAQLTDEFLR
                     FTIHYGCDVQVCNPRSGHEKGHVENKVGYVRYNFFSTSPIMDSFESLNKQLEEQLKAD
                     RKRTHYSKHQLIEDLWKKENKALHSLPQEDYPIFKEVEAKLNRYHEMKLDQTVIHIPK
                     AYGYSQLTVVLTWNKFKVLTPHGEILMNEYRPYMNKKRLIPWSDIMKQWSNKLNVVSY
                     SRYWKYLPARLQNYLAIDNNHLKYRRIKQLLSLLATYDIQQINEQFYELVVQEDAPSD
                     FDVNWSNYDALTKTGGVQK"
     gene            3448..4176
                     /gene="istB"
                     /locus_tag="U2K71_002573"
     CDS             3448..4176
                     /gene="istB"
                     /locus_tag="U2K71_002573"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS21-like element helper ATPase IstB"
                     /protein_id="HEM7316588.1"
                     /translation="MIGQTLEDYCKKLRLSHIITNIPRASFEAPEQFITDLFRLELET
                     RQQIKLERMEKLAKFPVPKSLKNYEWHEDIFLPSHLDKETLVSLDFLKRQENIVCVGS
                     PGTGKTHLAIALGRKACSEGVSTKFFRVNNLVEQLENALKKQQLASFKKRFETTELII
                     LDEMGYLPLTKEGAELLFQLISDWYEQKSIIITSNLEFSQWNRVFMDSRLTAALVDRL
                     IHHAHIISFKGESFRLKNSLSKSY"
     gene            complement(4304..5215)
                     /locus_tag="U2K71_002574"
     CDS             complement(4304..5215)
                     /locus_tag="U2K71_002574"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM7316589.1"
                     /translation="MKIKIERSNKEKIPKKKKERVVSVGKHRKMVLALWLLLSCSLAF
                     GIYKNFTAIDQHTTHEKVVVKEKIVNTSGVENFTVDFVKEYFSWKNNKEVIEKRMTNL
                     EQYLTEEGLALSQDMVRADIPTSSEVVSVKILDVEKHSEEFVVSFVVDQKITEGKKTQ
                     SISSAYRLMIFEDEKGNHIVTSLPTMIGKPEKAKHKVRQVKSDSKIDSKTTEEITEFL
                     ETFFRLYPAASEKELEHYVEKDAMQPINTNLKFVELVNPIYKQKGKAIQVIVGVEYLD
                     ELSKTTSVFQYRLKLYKNENWTISNCE"
     gene            complement(5232..6239)
                     /locus_tag="U2K71_002575"
     CDS             complement(5232..6239)
                     /locus_tag="U2K71_002575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HEM7316590.1"
                     /translation="MNLKKLSIISLTFLTISFVGILMCVGLLFGEDGEGSGSSGTSPG
                     GSSVSEVVLKHRSMVEKYAKESDITEYVSILLAIIQVESGGTMEDVMQSSESAGLSPN
                     SLSTEASIKQVCLYFSSLVKCSKELGCDQDSIIQAYNYGDGYLDYVAKNGKKHSFALA
                     ESFSKEKSGGQKVDYPNPIAIKENSGWRYNYGNMFYCLLVKQYLTTTQFDDKTVQGIF
                     DEAFKYEGTAYVFGGSSPGTGFDCSGLTQWCYAKVGLKLPRVAQDQYDAMTHIDLKDA
                     KPGDLIFFHSTYDAGTYVTHVGIYAGENRMFHAGDPVGWTNLTDSYWQQHIIGAGRYK
                     Q"
     gene            complement(6236..8365)
                     /locus_tag="U2K71_002576"
     CDS             complement(6236..8365)
                     /locus_tag="U2K71_002576"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HEM7316591.1"
                     /translation="MKKKLFRIVGIVFISIVTLTVLLSLVGTVAEATGLVDDTVEAGN
                     LYSKYSLNNYQLDFFVDSSWDWLPWNWGDGLGKSVMYGLYAITNFIWTVSLYLSNATG
                     YVVQEAYKLDFISDTAKSIGKNIQTLAGITENGLQTSGFYFGFLLLMILALGVYVAYT
                     GLLKRETTKAVRTVINFVMIFLLSGSFIAYAPTYITKINDFSSDVSEAALSLGTKIVV
                     PNSESQGKDSVDLIRDSLFSIQVQQPWLLLQFDDSNIEEIGEGRVNKILSVSPDENKG
                     KDREEAVKAEIEDNDNANLSITKTMNRLGTVVFLVLFNIGISFFVFLLTGIMLFSQIL
                     FIIFAMFLPISFLLSMLPTYESLGKRAIIRLFNTIMMRAGVTLVITTAFSISTMFFNI
                     SATYPFFMVAFLQIVTFAGIYFKLGDIMSMFNLQSNDSQSMGRRVMRKPQMLMNRKLR
                     QLNRNVGRTFAFGGGAAVGGKLATSSTKGKGSSKINRVSNRKNHSARSINELNSDLKK
                     QEQPVGTKKESRINLVGRKVGKVLDTKEIVKDKAKQAKNQVTDAPTNLKYSLHKGMEK
                     TKKVPEDFKRGPFEEKANRGELREKQRQRRDAKMDEKRKVLNEAWNRHEKGRENVPIK
                     KEIKPQKNELPENRSLKPRVQVHSNPEIKRKLSSQEVIPNGSTQPLSKNNSFQQVKQR
                     FTFPKRTNQQVQRKKNTRPKPKPGEKR"
     gene            complement(8370..>8490)
                     /locus_tag="U2K71_002577"
     CDS             complement(8370..>8490)
                     /locus_tag="U2K71_002577"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM7316592.1"
                     /translation="GQCLMQDLYGRVGVVQVHPVFEELFHAFDTRPPVQERME"
BASE COUNT         2599 a         1728 c         1486 g         2677 t
ORIGIN      
        1 attaaaaagt tagtaaataa ttgccctcta agttccacgg tctgttcatt aataacttta
       61 tatcctcgtt tttcaaaaaa tggctttgcg gtaatggaag cgtgggtagt gattttttgc
      121 ataccagttt ctaaaacatg gttttccaat tcatctgcaa ttttagtcgc aatacctttt
      181 ccttgatggc tatgtttttg tcacaacttc agtattcctg taacaatacg gatttcagta
      241 tacaacaaca gcttttgaaa agatacttgc aaaaggatta agatatctat gacctgctat
      301 gctcactgat gttgaaaaga atgttttatt aggattgagg taagcaagat agtgaatatg
      361 tccggaaagc ctgtaaaata aggcttttcg gacatatgtg aagttataaa aagatagcga
      421 aaaatctatt tagtatcact aatgttgcga acatatcgtc agaatagtct gacatttatt
      481 tcttgctaaa aatttcaaaa aagtcaagtg ctgacgcagc ggatgactat tttccagaaa
      541 attttagtat gtattcaaaa atcaaacgtg acaacagcta ttaactacat cctcatccag
      601 gtctgtaacg tttgaaccac tcttctgcgg gctcaaacat taagtaatgg aaggtacgtt
      661 tcaattgaaa cagcgtcgct gtcgtattca actcaatttt tatcacataa gtgagaagcg
      721 ttgcgattaa ggtgaggatg acttgattta tcgccccttg ttcactatgt gaatatagtc
      781 gtttaatggt cagattttgt tttaagtgtt tgaacaacaa ttcaatctgc caacgggctt
      841 gatacatgtc ggccacttcg tttggcgata catcaaagag attggtcacc attcgcagga
      901 ttttttgtcc cttggtaaga accgttacca acctaaaacg ctctgtcaca tggttgacac
      961 cgccgacaag aatcacttgt tggtcactga ttatcgtccc atcacgggtt gttgtatctg
     1021 ctacttcaat ttgatccaag atggtaattt ttgtattaga ttttgtccgt gtaacgaaga
     1081 aatagccgtc acgatttagt ttgtctaaca acttgtaatc aaagtagccg cgatccacca
     1141 catacgttgc ttgcgtttta tcaacaagaa cttcaaggtg attacggtca tgctcactcg
     1201 catttgtcat cgtaaaactt tcaggatact gattggtttt atcaagataa cagagattca
     1261 aatgtagctt gatgccagac tttgttttcc gaaacttggc ccatggaaac catgctttgt
     1321 tcaacggaat agtcgttgaa tcaatcaatt gtagactggt tgtttttgag cttggctgct
     1381 gagcagaaat tctggcgact aattgcgtaa aaatttccat caatacttct tgcttgattt
     1441 ctggcgctct acgagataac gagctgtaac acagggaatc aatgccaagt tccttgcaga
     1501 ggcgctgatc taaaaaggcg cgatcaatct cgcgatagct tggcaattct tcataaaccg
     1561 catgaagtaa cactttaagc gtcgtttgga agtctagttt tttactgtag aaatcaaagt
     1621 cgctaattaa gctttgagag ttttctgata aaccagaaaa atttattgca gaaaaccatt
     1681 tttgaaatga ggtttttacg ttatacttat ccatgcgtga gtccttttta ttggtcttgg
     1741 ataagtcatc cgagaatagt ataaaggact tttttgcgtt tggatagctt tgtattaggc
     1801 ttttgatggg ttaaaaagtc tttgcaacac tagtaattta gtatttatgt ataaattgaa
     1861 agatatttta ccaccactta gtcttcttaa aagtgatcca acattttgtt tgtggcagaa
     1921 tttaactaca cactaatgca gccgaaagta cacaactatg cagcagtttg actgcgaact
     1981 ggtttccttc catcataagt ttgaatgact tagtgatgga ggtacagaaa ggttgttaac
     2041 tttggatgat attaaaacaa taaaacattt gagaaataat cgtgatcagt caattgatca
     2101 gataagaaaa gcaatgaata tcaattggcg aacagcgaaa aaatacgccg atagtgatca
     2161 actattcatt acagatccca ctcgaaaaaa caaatccatg atggatgatg gttggggaga
     2221 aatcatcggt agttggctag aagaagacct acgtttacgc agaaaacttc gtagaacggc
     2281 caaagctatc catgaacaat tgaaaccttt gggtttccct ggctcctatc gaacagtttg
     2341 tgtatacatt tctcaatgga aaaagcagcg ttacgacgaa acgaattacc aagctgtcga
     2401 tcgtcttgat catccgatcg gagaagcaca ggtcgatttt ggaacaatgg aagttatgca
     2461 tgaagatcac atgaaagata tacaacttct tatcatgtca tttccctata gtaatgcgac
     2521 gtccgcaagt gccatgccag ctcaaaacca agagtgtttc ctggaagcat taaaacaact
     2581 atttaatcaa tgcggcggcg ttccaaaaac gatcagaata gataacttaa ctccagcggt
     2641 gaaaaaaaca aaatcaaaat ttgaagaagc acaactaacc gatgaattct tacgtttcac
     2701 cattcattat ggttgtgatg ttcaagtctg taacccacgt agtgggcatg aaaaaggaca
     2761 tgtcgaaaat aaagtgggtt atgtccgata caatttcttc tcgacttcgc caatcatgga
     2821 ctcttttgag tccctaaata agcaacttga agaacaatta aaagcagatc gaaaaaggac
     2881 gcattattca aaacatcaat taatcgaaga tttgtggaag aaagaaaata aagcgttaca
     2941 ttctcttcca caagaggatt acccaatttt taaagaagtg gaagctaaac taaatcgtta
     3001 tcacgaaatg aaattggatc aaacggttat tcatattcct aaagcatatg gctattctca
     3061 attgactgtt gttttgactt ggaataaatt caaagttctt acgcctcatg gagaaatatt
     3121 aatgaacgaa tatcgcccct atatgaacaa aaaaagattg atcccatgga gtgacatcat
     3181 gaaacaatgg agtaataaat tgaatgtcgt tagttattcg aggtattgga agtacttgcc
     3241 tgcacgcctt caaaattatc tagcgattga taataatcac ctgaaataca gacggataaa
     3301 acaattacta agtttattag cgacctacga tatccaacaa attaatgaac aattttacga
     3361 attagtcgtt caagaagacg ccccctctga ctttgatgtg aattggtcaa actatgatgc
     3421 attaacaaaa acaggaggtg tgcaaaaatg attggacaga cgttagaaga ttattgtaaa
     3481 aagcttcgat taagccatat catcacgaac ataccaagag cttctttcga agctccagaa
     3541 caatttatta ccgatttatt ccgtttagaa ctggagacca gacagcagat caaattagaa
     3601 cggatggaaa aattagccaa atttcctgta ccaaaatcgc taaagaacta tgaatggcac
     3661 gaagatattt ttctaccatc tcatctagat aaggagaccc ttgtctctct tgattttcta
     3721 aaaaggcaag aaaacattgt ttgtgttggc tctcctggta cagggaaaac acatctagcg
     3781 atcgcattag ggagaaaagc ctgtagtgaa ggtgtttcaa ccaaattttt ccgagttaac
     3841 aatctcgtag agcaattaga gaatgcatta aaaaaacaac agctagccag tttcaagaag
     3901 cgattcgaaa caacagaatt aattattctt gatgaaatgg ggtacttacc attgacaaaa
     3961 gaaggagctg aacttctctt ccaactgatt tcagattggt atgagcaaaa gagcattatt
     4021 attacctcta atttagaatt cagtcaatgg aatcgagtgt ttatggattc tcgattaaca
     4081 gctgctctcg ttgatcggct aatccatcac gctcacatta tcagcttcaa aggcgaaagt
     4141 ttcagattga aaaattcatt atctaaatca tattgaagga aaaaactagt tgcagcaaaa
     4201 ctctgcactt tcggctgcat ttttttgtaa aaagctattg cacaacacaa acatttgaac
     4261 gacgtaattc caaatgttgg attgctttta gataatcttt tatttattca caattgctaa
     4321 tagtccaatt ttcatttttg tatagtttta atctatactg aaaaacactt gttgttttgg
     4381 acaactcatc aagatactca acaccaacaa tcacttgaat cgctttaccc ttttgtttgt
     4441 aaataggatt cactaattcc acaaatttca aattagtatt aattggttgc atcgcgtctt
     4501 tttcaacata atgctctagt tctttttctg aggctgctgg ataaagcctg aaaaacgtct
     4561 ctaaaaactc ggtgatttct tccgttgtct ttgaatcaat ttttgaatca ctttttactt
     4621 gtcttacttt atgttttgcc ttttctggct taccaatcat agtgggtaaa ctcgttacaa
     4681 tatgattgcc tttttcatct tcaaagatca tcagacgata tgcagaagaa attgattgtg
     4741 tctttttccc ttctgtaatc ttttgatcta ccacaaatga aacgacaaat tcttccgagt
     4801 gtttttcaac atctagaatt tttactgata caacctcgga actagtggga atatccgctc
     4861 gaaccatatc ttggctcaaa gcgagccctt cttctgtaag gtactgctca agattagtca
     4921 ttctcttttc aattacctcc ttgttattct tccatgaaaa atattctttc acaaaatcta
     4981 cagtaaaatt ttctactcca gaggtattca ctattttctc tttcacgacc actttctcat
     5041 gagttgtatg ctgatcaatg gccgtgaagt ttttgtagat tccaaaagca aggctacaag
     5101 acaataaaag ccaaagggca agcaccattt ttcgatgctt gcccacggat actactcgtt
     5161 ctttcttctt ttttggaatc ttttccttgt tacttcgctc aatttttatc ttcattttcc
     5221 attcctcttt tctattgttt ataccgacca gcaccaataa tgtgctgttg ccaatagcta
     5281 tcggtaagat tggtccaacc caccggatct cctgcatgaa acattctgtt ctctcctgca
     5341 taaatgccca catgagtcac ataagtaccg gcatcataag ttgaatgaaa gaaaatcaaa
     5401 tcgcctggtt tggcatcttt taagtcaata tgagtcatag catcatattg atcctgagcc
     5461 acccgaggga gtttcaaacc aactttggca taacaccatt gagttaaacc actacaatca
     5521 aacccagttc cgggacttga cccaccaaat acataagccg ttccttcata cttgaacgcc
     5581 tcgtcaaaaa ttccttggac tgttttatca tcaaactgtg ttgtagttag gtactgcttc
     5641 actaataagc aataaaacat attgccatag ttataccgcc acccactatt ttccttaatc
     5701 gcgatgggat tgggataatc gactttttga ccacccgatt tttcttttga aaaagattcg
     5761 gctaaagcaa aactatgctt ctttccgttt ttcgcgacat aatccaaata cccgtcacca
     5821 tagttataag cctgaatgat actatcttga tcgcatccta gttctttgga acacttaacc
     5881 aaagacgaaa aatagagaca cacttgtttg atggatgcct cggtacttaa tgaattcggt
     5941 gataaacctg ctgattcaga actttgcatg acatcttcca ttgtacctcc agattcgact
     6001 tgaataatcg ccaaaagaat cgaaacatac tctgttatat ctgattcttt ggcatacttt
     6061 tccaccattg aacggtgttt caaaactact tctgagacac tactacctcc aggagaagtt
     6121 ccagaactac cacttccctc accgtcctca ccaaacaaca aacccacaca catcaagatg
     6181 cccacaaaag agatggtcag gaaggttaac gaaataatac tcaatttttt taagttcatc
     6241 ttttctcacc tggctttggt tttgggcgag tgttcttttt tctttgtact tgttgattgg
     6301 ttcgctttgg aaaagtgaat ctctgtttca cttgttgaaa ggaattgttc ttgcttagtg
     6361 gctgagtgct tccgtttgga atgacttctt gactagaaag tttccgtttg atttcaggat
     6421 tcgaatgtac ttgtaccctt ggttttaaag acctattttc tggtagttca tttttctgtg
     6481 gttttatctc cttttttatc ggaacatttt cccttccttt ctcatgacga ttccatgctt
     6541 cgttcaatac tttgcgtttt tcatccattt ttgcgtctct tcgttgtcgt tgtttttctc
     6601 gcaactcccc tcgatttgct ttttcttcga aaggccctcg cttaaagtcc tctggtactt
     6661 ttttcgtctt ttccatgcct ttatgaaggc tgtatttcaa gttagttggt gcatcggtta
     6721 cttgattttt tgcttgtttc gctttgtcct tcacgatttc ttttgtatcg agtacttttc
     6781 caacttttct accaactaaa tttattcgtg attctttctt cgtgcccact ggttgttctt
     6841 gttttttcag atcagaattt agctcgttaa tgcttctcgc ggaatggttc tttctattgg
     6901 acactctatt gatttttgaa ctacctttcc cttttgtaga actggtagct agtttcccac
     6961 caactgctgc accgccacca aaagcaaaag ttcgacccac atttcgatta agctgtcgca
     7021 attttcgatt catcaacatt tgcggtttcc gcatgactcg ccttcccatt gactgactat
     7081 cattactttg aaggttaaac atgctcataa tgtcacctaa tttgaaatag ataccggcaa
     7141 aggtcacaat ttgtagaaaa gctaccataa agaagggata ggtggccgaa atattgaaaa
     7201 acatcgtgga gatactgaat gcagtcgtaa tgactaaggt aactcccgct cgcatcataa
     7261 tcgtattgaa caaacgaata atcgcccttt tgcccaaact ctcatacgtc ggcaacatcg
     7321 acaataagaa actaatgggc agaaacatcg caaagataat gaaaaggatt tgtgagaaca
     7381 acataatccc tgtcagaaga aacacaaaaa aggaaatccc aatattaaac aacactaaaa
     7441 agaccacggt tcctaaacgg ttcatggtct tggtaatact gagattcgca ttgtcattat
     7501 cttcaatctc tgctttgact gcttcttccc gatccttacc cttattttca tccggactca
     7561 cggataaaat cttattaacc cgaccctcgc caatttcttc tatattggaa tcatcgaatt
     7621 gtaatagtaa ccagggttgt tgaacttgaa tcgaaaataa actgtctcga atcaaatcca
     7681 cactatcttt tccctggctt tctgaattag gtacaacaat cttggttcca aggctaagag
     7741 ccgcttcact aacgtctgag ctaaagtcat tgatttttgt aatataggtg ggcgcatacg
     7801 caatgaaaga acccgagagt agaaaaatca tcacaaaatt aatgacggtt cggactgctt
     7861 tggtagtttc tcgttttaat aatccggtat acgccacata aactcctaga gctaaaatca
     7921 tcaataatag gaacccaaag tagaatcccg aagtctgtaa tccattttca gtgataccgg
     7981 ctaaagtttg gatattcttt ccaatacttt tagccgtatc cgaaatgaaa tccaacttgt
     8041 aagcttcttg aaccacatag cccgtggcgt ttgataaata cagacttact gtccaaataa
     8101 aattggtaat ggcataaaga ccatacatca cgcttttccc taagccatcc ccccaattcc
     8161 aaggtaacca atcccaagaa ctatccacaa agaaatccag ttgataattg ttcagagaat
     8221 atttggaata aaggtttccg gcttcaactg tatcatcaac cagacctgtc gcctcagcta
     8281 ccgtaccgac taaactcaat agaactgtca gagtgacgat gctaataaaa acgattccca
     8341 caatccgaaa aagtttcttt ttcatcccct cactccattc tctcttgaac cggcggacga
     8401 gtgtcaaaag catggaagag ttcttcaaag actggatgaa cttgaaccac acctactcgc
     8461 ccatacaagt cttgcatcaa gcattgaccg
//
LOCUS       DASHHB010000088         8354 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.092, whole genome shotgun sequence.
ACCESSION   DASHHB010000088 DASHHB010000000
VERSION     DASHHB010000088.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8354)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8354)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8354
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.092"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(677..1717)
                     /locus_tag="U2K71_002578"
     CDS             complement(677..1717)
                     /locus_tag="U2K71_002578"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM7316593.1"
                     /translation="MNDFNYYKSKEIYREKYYQMPKVFFTNEKYMDLSNDAKIAYMLL
                     KDRFDYSVKNNWVDSDDNIFFIFTVEELMKLLQCREGKVSKIKKELEKAGLLKQKRGR
                     VNKRDGKIESMPNLLYLGKPEVTNQDVFKIMEEEDNTDTTVIAKIANTAKPREINDTT
                     VIAKIANTAKSSHIKDSTVIAKIADNLFYSNSIDTNRHIIDTETDQLQNQVLLDNFVD
                     IMKEDSINTFVPENVLNLIKTFSSTYEDAQKTVQTIHNAKKKAEELEQTVVVYEELES
                     YGIDADKGLYMTLLKAYQKQKTEKVNNLQNLIFVYVKNWFVEKAIAAKLANEQRETFN
                     ELPTVSTENWLE"
     gene            complement(2066..2392)
                     /locus_tag="U2K71_002579"
     CDS             complement(2066..2392)
                     /locus_tag="U2K71_002579"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316594.1"
                     /translation="MAEDNRSQAAVSRLLGNKNKKNFKSKEVEEIELTKKNISKVIKD
                     QGSSVRTADSIRINPILMSALKYWTTIAESDKSKPDVIEDALLKSIPEEYLIEGYKLA
                     KKQNKI"
     gene            complement(2379..3182)
                     /locus_tag="U2K71_002580"
     CDS             complement(2379..3182)
                     /locus_tag="U2K71_002580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM7316595.1"
                     /translation="MGDILMARILSVANQKGGVSKTTTSVNLAACLKFVYGKNVLLVD
                     IDPQGNATDNVGFDIDGTNQPTIYEVLKGEADITDAILDYKGIDVLPADIALSSAERE
                     FTQVGSEHRLKRVLQPIKDNYDYIIIDCPPSLGMLTVNAFTVSDEIIIPVEAAYFSLK
                     GLIKLSETIETVKEYTNDKLEVRGVLFTKYNKRYNISKEMTSAATEISRVIGAKIFKT
                     YIRRTITVDEAQAAGSDLINFNKPSTAEDDYKAFTEEYLKEVGDFDGRR"
     gene            complement(3535..3825)
                     /locus_tag="U2K71_002581"
     CDS             complement(3535..3825)
                     /locus_tag="U2K71_002581"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000026576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type III secretion system protein PrgN"
                     /protein_id="HEM7316596.1"
                     /translation="MSINTFVYSHPINVYIIKNLGITVEQFCELYAYPQGTVASWITR
                     QRRIKSLPASFVYDLSLASSLNMSDVYEKLLSLEKEYDSFKIKHDKKIKKHI"
     gene            complement(3961..4140)
                     /locus_tag="U2K71_002582"
     CDS             complement(3961..4140)
                     /locus_tag="U2K71_002582"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001021554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316597.1"
                     /translation="MNIIQQYELKYITFDQLSEEIWGYGQRLINEVGVERFSFYVEAA
                     AGYHNFRFYIFPLYI"
     gene            complement(4137..4487)
                     /locus_tag="U2K71_002583"
     CDS             complement(4137..4487)
                     /locus_tag="U2K71_002583"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316598.1"
                     /translation="MIDYNDRGRLKWAGFYLSDHTEEIDADNEQRTSQNLAKEQMTTE
                     EIAEVLNAAVIKNRSVSIQKEERNAEGYYPEDIIGKILGSDELGIYVGKDKIHYDEIR
                     HIEFYQDNKWSRLE"
     gene            complement(4480..5805)
                     /locus_tag="U2K71_002584"
     CDS             complement(4480..5805)
                     /locus_tag="U2K71_002584"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Y-family DNA polymerase"
                     /protein_id="HEM7316599.1"
                     /translation="MMDYSKEPVNDYFLIDMKSFYASVECIERNLDPLTTELVVMSRS
                     DNTGSGLILASSPEAKKRYGITNVSRPRDLPQPFPKTLHVVPPRMNLYIKRNMQVNNI
                     FRRYVADEDLLIYSIDESILKVTKSLNLFTTEETRSQRRKKLAQMIQERIKEELGLIA
                     TVGVGDNPLLAKLALDNEAKHNEGFIAEWTYENVPEKVWNIPEMTDFWGIGSRMKKRL
                     NQMGILSIRDLANWNPYTIKNRLGVIGLQLYFHANGIDRTDIAIPPEPTKEKSYGNSQ
                     VLPRDYTRRNEIELVVKEMAEQVAIRIRQHNCKTGCVHLHIGTSILETRPGFSHQMKI
                     PITDNTKELQNYCLFLFDKYYEGQEVRHVGITYSKLVYTDSLQLDLFSDPQKQIDEEN
                     LDKIIDKIRQKYGFTSIVHASSMLEGARSITRSTLVGGHAGGNGGIKND"
     gene            complement(5974..6558)
                     /locus_tag="U2K71_002585"
     CDS             complement(5974..6558)
                     /locus_tag="U2K71_002585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007207927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7316600.1"
                     /translation="MKIGYARVSTGLQNLDLQEDSLNKFGCEKIFTDHMSGAKSNRPG
                     LEMAIDFVRSGDTLVVWRLDRLGRNMEDLISIVNRLNERGVSFHSIQENITMDKSSST
                     GQLMFHLFAAFAEFERNLILERSAAGRAAARARGRFGGRPEKLSEQDLELLKTLVDSG
                     TPIKTIAERWNVSRTTIYRYLAKMEEKNGKTNSH"
     gene            complement(6769..6972)
                     /locus_tag="U2K71_002586"
     CDS             complement(6769..6972)
                     /locus_tag="U2K71_002586"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316601.1"
                     /translation="MPEFVNTPNFYQDSDILSKINLPKEESALQVQRTLQETQKIQKS
                     ISNLEDSEEKRKKRDQKTYQKEK"
     gene            complement(6982..7404)
                     /locus_tag="U2K71_002587"
     CDS             complement(6982..7404)
                     /locus_tag="U2K71_002587"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316602.1"
                     /translation="MKNKFILSLGILTIGISVTGTTIPTFADSTNEPTTVVDNNQELT
                     PLDRNGIAIGNPTNDLTVTENYLIVSPLLARAASKTIRVTGTMIFDKVGWPSRPPQSR
                     NWKEKRNGYWYYGKLYLQSYKSAGNNWIATYSGTLTRK"
     gene            7718..8332
                     /locus_tag="U2K71_002588"
     CDS             7718..8332
                     /locus_tag="U2K71_002588"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001261742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7316603.1"
                     /translation="MRVAYIRVSSIDQNEQRQIEEMKKFGAERIFIEKQSGATITHRP
                     VFQEALDYVRDQDIFIVEAIDRLGRNYDEIITSVNYLKKKNVKLIITSLPIMAEAIGN
                     PLLDKFIKDLIIQILAMIAEQERTESKRRQAQGIKIAKANGVYKGRPKLYSADAKDPQ
                     RRLVYKSIVEDLKNGVAIAKIAKDYNVTRQTVYRIKKDSMVNDK"
BASE COUNT         2576 a         1509 c         1249 g         3020 t
ORIGIN      
        1 cactctcttc gggttttcgg tctgtactga aatcaaggta ttattgggaa tcccagctta
       61 aatcatagat accgtaaggg attttattct ttatttaaaa ctttgcaaca gaaccgtccg
      121 ttgcgggtaa taccgtaaca ctgaagggca cgcattaagg cgtcatctta atgctttcct
      181 gtaggacact gacggagatt accagtctcc cgagccttac aggggccttt cttttttttg
      241 tattttcaat tatcacaatt tatcgtccaa aagtgcagaa cgaaaatcaa ttcatcacaa
      301 gcatatcaga caattaaatt taaaacaact aaaaatatac aaaaaagtgt aattgaatat
      361 tagcagcact gcctgaaaag aagtaatatc aggcttttca gcttggggaa ataagtgaat
      421 tacctctctt catgaattat tataactcaa aaaagcacat atgcaaacac tagttcccta
      481 tttttttgaa tgaaaaataa ctaaaatatc taacgattat gactttaaaa cctacgtttg
      541 tgattgtttt cataaacttt taatctaaaa actccggaat aatagctatt tatttgtaac
      601 atttgtaata aaattaaaat cataaaaaag ttctaattta aaattgatca tttttatagt
      661 gatcgctttt tatcaactac tctagccaat tttccgtact aactgttggt aattcgttaa
      721 atgtttctct ttgttcgttt gcaagctttg ccgcaatagc tttttcgaca aaccaatttt
      781 ttacataaac gaaaattaga ttctgcaaat tgtttacttt ttcagttttt tgtttttgat
      841 aagctttcag caaagtcata tataatcctt tgtcagcatc aattccataa gattccagtt
      901 cttcataaac aacaactgtt tgctctaatt cttcagcttt tttctttgca ttatgaatag
      961 tttggacggt tttttgagcg tcttcgtaag tactagaaaa tgtttttatc aagtttaata
     1021 cattttctgg tacaaatgta tttatactgt cctctttcat gatgtcgacg aagttatcta
     1081 gtaaaacttg attttgtaac tgatcagttt cagtgtctat tatgtgtcta ttagtgtcta
     1141 tagaattaga atagaataga ttgtctgcga ttttcgcaat tacggttgag tccttgatat
     1201 gactggattt tgccgtattt gcgattttcg caattacggt ggtatcattg atttctcgcg
     1261 gttttgccgt atttgcgatt ttcgcaatta cggtggtatc agtattgtct tcctcttcca
     1321 tgattttaaa gacgtcttga ttcgttactt ctggcttgcc taagtacaat aaattaggca
     1381 tggattcaat cttaccgtca cgtttattta ctcttcctct cttctgtttc agaagacctg
     1441 ctttttctaa ttcctttttg attttagata ctttaccttc gcggcattgt aacaatttca
     1501 ttaattcttc aactgtgaat atgaaaaata tattatcatc agaatctacc cagttatttt
     1561 tgactgaata atcaaatcga tctttaagta gcatataagc aatttttgca tcatttgata
     1621 gatccatgta tttttcgtta gtaaaaaata ccttaggcat ttgataatat ttttcacgat
     1681 aaatttcttt tgatttgtaa taattaaaat cattcatagc caatcctctt tctgtaggat
     1741 agcgttttgt tcaaaactat gttacaatga attaaacaaa tatatctatc cactatatat
     1801 ttttctactg ctgataagtc gccaaacttt gttaaagcag tagtcttttt ttatcttttt
     1861 ttagtttacc ttaaaaaata aattaaagca aaccgctcta tttcaaggtt taagctcata
     1921 gtaactatat cacgatatta caataatatt atatcacgat atcgtaatat tacaatatga
     1981 agataagcga cttatcagct ttgatactac tgatttttat aaaaaaaata tcacgatatt
     2041 atgatatcgt gatattgaat gtaaattaaa ttttattttg tttctttgct agtttatatc
     2101 cttcaatcaa gtattcttca ggtatggact ttaatagcgc atcttctata acgtctggtt
     2161 tacttttgtc tgattcagca atagtcgtcc aatatttgag tgctgacatt agaattggat
     2221 ttatacgaat gctatctgct gttcttacac tacttccttg atcttttatg actttactta
     2281 tatttttctt ggttaattcg atttcttcta cttcttttga tttaaaattc tttttatttt
     2341 tatttcctag taatctactt actgcagctt gactgcgatt atcttctgcc atcaaaatca
     2401 ccaacttctt ttaaatattc ttctgtaaat gctttatagt catcttcagc tgttgagggt
     2461 ttattaaaat tgattagatc tgatccagca gcttgcgctt cgtcaacggt tattgtccta
     2521 cgaatataag ttttgaaaat ttttgcacca atgactctag aaatttcggt tgctgcagac
     2581 gtcatttctt tagaaatgtt ataacgctta ttatacttag taaatagaac tcctcgtact
     2641 tcaagcttgt cgttcgtata ttcttttacc gtttcaatag tttcacttaa ctttattagt
     2701 ccttttaatg agaaatacgc agcttcaact ggaatgataa tttcatcaga aacagtaaat
     2761 gcgttgactg taagcattcc taaacttggt ggacaatcaa ttataatata atcatagttg
     2821 tccttgattg gttgaagtac tcttttaagc ctgtgttcac ttcctacttg agtaaattcc
     2881 ctctctgcag aagaaagggc tatgtcggct ggcaaaacat caattccttt atagtccaga
     2941 atagcatctg taatgtcagc ctcccctttt aatacttcat aaattgtagg ttggtttgtt
     3001 ccatctatat cgaaacctac attatctgta gcatttccct gaggatcaat gtccactaaa
     3061 agtacatttt taccgtaaac aaatttcaaa catgctgcta aattaacgct agtcgtagtt
     3121 ttagaaactc cacctttttg attggctact gacaatattc ttgccattaa aatatccccc
     3181 attcatctta tattataata taattatatc ataatattgt gatattgcaa tatcgtatat
     3241 gcattatatt gtaatattgt gtgattattt tgttttaaag atatatatcg gttcggatat
     3301 aaagattatt catatgttat tcgaaattaa aataatcact ttttattact ttaaaatttt
     3361 ttattaatga taagaaatat gttttgcgag cagcaactag aataatttaa acattactaa
     3421 actgtctata ttgttactaa taattccctt cttatagtgc attttcttag aaaaaaacaa
     3481 tattacgata tcatgatatt aagatatcgt aatattatac aattgtgatt gatattatat
     3541 atgttttttt atttttttat catgtttaat cttgaaggaa tcatattctt tttctaaaga
     3601 caggagcttt tcataaacat cagacatgtt taaactagag gccaggctta aatcataaac
     3661 gaatgaagca ggtaatgatt ttattctacg ttgtcgggtt atccagcttg caaccgttcc
     3721 ttgtgggtag gcatataact cacaaaattg ttctacagtg attcctaaat tcttgataat
     3781 gtaaacattg atcgggtgcg agtaaacaaa tgtattgata gacatggtgc gtaagcctac
     3841 tttcttttat tattgcgttt gcaataatag tttaacacac gcattattct agtatctact
     3901 cctatttgta aaaacaaaaa aggattcata gagataactc tatagaatcc gtaaaatacc
     3961 ttatatatat aacgggaaga tataaaacct aaaattatga taacctgctg cagcttctac
     4021 atagaaagag aagcgctcaa caccaacctc atttatcaaa cgttggccat aaccccaaat
     4081 ctcttctgat agctgatcaa aggttatata ctttagctca tattgctgga taatgttcat
     4141 tctaatcgac tccatttatt atcctggtaa aattcgatat gacgtatttc gtcgtagtga
     4201 attttgtctt ttccaacgta gatccccagc tcatcactac ctaagatctt gccaatgata
     4261 tcttctggat agtatccttc agcgtttcgt tcttcttttt gaatagatac tgatctattt
     4321 tttatgactg cagcgttcaa tacttccgca atctcttcag tcgtcatttg ttcttttgct
     4381 aaattttgac tggttcgttg ttcattgtcg gcatcaattt cttcagtatg atcagataaa
     4441 taaaaccctg cccactttaa acgtccacga tcattgtaat caatcatttt taattccccc
     4501 atttccgccc gcatgaccac caacaagtgt gcttctagta atgcttctag cgccttctaa
     4561 cattgaagaa gcatgtacaa tcgacgtaaa accatatttt tgccgaatct tgtcaataat
     4621 tttatctaaa ttttcttcgt caatttgctt ttgtggatca ctaaataaat ccagctgaag
     4681 cgaatcggtg tacacaagct tgctataggt tattccaaca tgccttactt cttgtccttc
     4741 ataatactta tcaaacaaga aaagacaata attctgcaat tcctttgtat tatctgtgat
     4801 agggatcttc atctggtgtg aaaaccctgg tctcgtttct aagatcgacg tgccaatgtg
     4861 taaatggaca caacctgttt tacagttatg ctgcctaatg cgtatcgcta cttgttccgc
     4921 catttctttt accaccagct cgatttcatt tcttctagta taatctcgtg gtaacacttg
     4981 tgaatttcca tatgattttt cctttgtcgg ttctggtggg attgctatat ctgttcgatc
     5041 gatcccatta gcatggaagt acagctgtaa tccaatcaca cctaatctat tcttaatcgt
     5101 ataaggattc cagttagcaa ggtctctaat gctcaaaatt cccatttggt tgagcctttt
     5161 tttcattcgc gaaccaattc cccaaaagtc cgtcatttcg ggaatattcc atactttctc
     5221 aggaacgttt tcgtacgtcc attccgcaat aaatccttca ttatgctttg cttcattgtc
     5281 taaggctaat ttagccaaaa ggggattatc gccgacgcct accgtagcaa tcaaacctaa
     5341 ctcttcttta atacgttctt ggatcatttg agcgagcttc ttccttcgct gacttcgtgt
     5401 ttcttcagtc gtaaaaagat tcagtgattt tgtcactttt aaaatcgatt catcgatcga
     5461 gtaaatcagt agatcttcat cagccacata tcttctgaaa atattattta cctgcatatt
     5521 tcgcttgata tataggttca tacgtggtgg aacaacgtgt agtgttttag gaaatggttg
     5581 tggtaagtca cgtggtctac tcacatttgt aataccatac cgcttttttg cttcaggaga
     5641 agaagctaat atcaatcctg aaccagtatt atcagatcga ctcataacaa caagttctgt
     5701 tgttaatgga tctaaatttc tttctataca ttcgacactt gcgtaaaagc ttttcatatc
     5761 gattaaaaag taatcattta ctggctcttt tgaataatcc atcataaata acactccatt
     5821 cttccacatt atacaaacat atgttcgatg ttgtaaagaa gaaatagcta aatatttgag
     5881 aataagttat cagtagacac ttacaaaaaa ttctcatact attgtcactt ttctagataa
     5941 atggcaaaaa ttttcagcat taaatgacaa aaactaatga gaatttgtct ttccattttt
     6001 ctcctccatt tttgccaaat aacgataaat ggtggttctt gagacgttcc accgttccgc
     6061 aatagtctta ataggtgtac cgctatccac taaagtcttc agtaattcca aatcttgttc
     6121 agacaatttt tcaggacgac caccaaaccg tcctcttgca cgagcagctg ctcgtcctgc
     6181 tgcggatcgt tccaggatca aattacgctc aaattctgca aatgccgcaa acaaatgaaa
     6241 catgagctgt cctgtagaac ttgatttgtc catcgtaata ttttcttgaa tactatgaaa
     6301 actcacacca cgctcattca gtcgattgac gattgaaatc aaatcttcca tatttctacc
     6361 taaacgatct aagcgccaaa cgacaagtgt atcgccagag cgaacaaaat caatggccat
     6421 ttctaatcct ggtcgattac tttttgcccc actcatatga tcagtaaaaa ttttttcaca
     6481 accaaattta tttaggctgt cttcttgtaa atccaaattt tgtaaaccag tagatacccg
     6541 ggcgtatcct attttcaaaa aaatcactcc ttcattaatt ttattgtaac ataactttat
     6601 ccatatagtt taactgaaca tagatattgt tatgggttta tgaacatgaa atgaattgtt
     6661 tttttgaaaa ataaagcaaa aaaagaaagt gtccagaaat gggtgatttt atgaacactt
     6721 tcaattagaa aaaaagaatg tcgactagac actctttttt taaaatcatt atttttcttt
     6781 ttgatatgtt ttttggtctc ttttttttct tttttcttca gaatcttcta gattagaaat
     6841 tgatttttga attttttgtg tctcctgtaa agttcgctga acttgtaaag ctgattcttc
     6901 tttcggtaaa ttaattttac ttagaatatc gctatcttga taaaaatttg gtgtgttcac
     6961 aaattctggc ataaaaaatc cctactttct agttaaggtt cctgaataag tcgcaatcca
     7021 attgtttcca gctgatttat aactttgtaa atacaatttt ccatagtacc aataaccatt
     7081 tcttttttct ttccaattcc gtgattgagg aggtctagaa ggccaaccaa ctttatcaaa
     7141 aatcatcgtt cctgtcactc gtatcgtttt agaagctgct cgagcaagta atggagaaac
     7201 tattaaataa ttttcagtaa cagttaagtc gttagttggg ttaccaatgg ctattccgtt
     7261 cctatctaaa ggagttaatt cttggttatt atctactaca gtagttggtt catttgtact
     7321 atcagcaaat gtaggaattg tagttccagt tacactaatt ccaatagtta aaatacctaa
     7381 agataaaata aacttatttt tcattgtcta tcacctcgaa atcaaaaata agaaaacgtt
     7441 ttcttaataa atcttaacag ataaatcaag ttatatacta gaaaattcat aaaatatatt
     7501 tttaatgtca taatcgttta aattttcttg attgacattt atttacaaaa aatattgcca
     7561 tttaaaaaga aaagtgacac atactatttc ctccatcatt agtttttgtc tatataaggt
     7621 ttaccctaaa tagacactgg tataacattg aaaaatatgg cttcctcgtg tatgattagg
     7681 gtgtatttta aatagttgca gaaagtaggt aaaagttttg agagttgcat acatacgtgt
     7741 ctcatcgatc gatcaaaatg aacaacgcca gattgaagaa atgaaaaaat tcggtgcaga
     7801 gagaattttc atcgagaaac aatctggcgc aacgattact catcgacctg ttttccaaga
     7861 agcattagat tacgtccgag atcaagatat ttttattgtg gaagctattg atcgacttgg
     7921 aagaaattat gacgagatca tcacttcagt caattacttg aagaagaaaa atgtgaagct
     7981 gatcattact agtttaccga tcatggctga agcaattggc aatcctctcc tggataaatt
     8041 cattaaggat ctgatcatcc aaattttggc catgatcgct gaacaagaac ggactgaatc
     8101 gaaacgtcgc caggcacaag gaattaagat cgcaaaagca aacggcgtct acaaagggcg
     8161 gcccaaatta tacagtgcag acgcaaaaga tccccaacgt cgccttgttt acaaaagcat
     8221 cgttgaagat cttaaaaatg gtgttgcgat tgctaagatt gctaaagact acaatgttac
     8281 gagacaaacc gtgtatagaa taaaaaaaga cagcatggtc aatgacaaat agttgatcat
     8341 gctgctttta ttat
//
LOCUS       DASHHB010000089         8276 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.093, whole genome shotgun sequence.
ACCESSION   DASHHB010000089 DASHHB010000000
VERSION     DASHHB010000089.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8276)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8276)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8276
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.093"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..382
                     /locus_tag="U2K71_002589"
     CDS             <1..382
                     /locus_tag="U2K71_002589"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="2-dehydro-3-deoxyphosphogluconate aldolase"
                     /protein_id="HEM7316604.1"
                     /translation="NSLRYLEFREGAFDFQTSQQNLLTIPGAMSPTEIFKMFEMGADI
                     VKVFPANCLGPEYFNQVQAPLGSLPLMAVGGVDQTNAQNYLNNGASYVGIGSKFFEKS
                     AVHQLNYERLMELAESFIDSLRVE"
     gene            complement(776..1783)
                     /locus_tag="U2K71_002590"
     CDS             complement(776..1783)
                     /locus_tag="U2K71_002590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010780432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7316605.1"
                     /translation="MVTIRDIAKKSGYSTATISRLFKGDESLSITSDTKNKIIVTAKS
                     LGYDLTKIKTTLYKIAILFWISEEQLKKDVYYQHVKDDLKNYSKKANLDLIFISKNDG
                     VKSIPDDITGFIAIGQLTKKELYYLNQKGYKGVIRGINPLPEHFDTVEPDFHEMARQA
                     INYFINEGFTKIGFIGGNFFNLETGKEEIDSREYAFRYHLEKSGLLNEKYIFSQGSFT
                     VDEGYQLTLKMKAELNEDMPEACFIPSDTIAAGVLQGCHELGINIPKDMAVISINDDD
                     IAKFVAPPLTTFKIDIEEIAKTCVDLLSDQLVYSRDISKKTLIGAKLTVRKSFIPKEE
                     V"
     gene            2250..3656
                     /gene="lacG"
                     /locus_tag="U2K71_002591"
     CDS             2250..3656
                     /gene="lacG"
                     /locus_tag="U2K71_002591"
                     /EC_number="3.2.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333907.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phospho-beta-galactosidase"
                     /protein_id="HEM7316606.1"
                     /translation="MNYKTLPEDFIMGGATAAYQAEGSTKEGGKGQVAWDTFLVEKGR
                     YTADPASDFYNQYPVDAELCKKFKIDSIRMSIAWSRIFPKGYGEVNQTGVEYYHKVFA
                     ELNKHGIIPFVTLHHFDTPKELFDNGDFLNRENVEHFVNYAQFCFDEFSEVHYWSTFN
                     EIYPVASNQYLIGSFPPEVQFDIEKTVTCLHNMMYAHAKTVNIFKDGGYKGEIGVVHS
                     LETKYPASDKEEDKHAAKLDDALSIKFLLDATYLGYYSDETMAHINEILAANGQSADI
                     DSNDFREMKKAANRNDFLGINNYGSHWVKEYKGESTLHYNTTGEKGSQVYCIKGIGER
                     IVREDLPKTDWDWIIYPKGLYDLLVRIQDEYPNYNKIYITENGLGYKDNFEDGIILDD
                     DRIDYLRQHFNAISDAIKMGVNVKGYFIWSLMDVFSWVNGYTKRYGLFYVDFDTQKRY
                     PKESAYWYKFVSETKTII"
     gene            3679..4002
                     /locus_tag="U2K71_002592"
     CDS             3679..4002
                     /locus_tag="U2K71_002592"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose/cellobiose transporter subunit IIA"
                     /protein_id="HEM7316607.1"
                     /translation="MDRKELQLLGFEIVAYAGDARSSLLTVLKEVREGNFDNVERLLA
                     DADENLTLAHNSQTKCLAEEASGNEMEVGVIFIHGQDHLMTTILLRELINDFVELYKE
                     KFAKA"
     gene            4013..5695
                     /locus_tag="U2K71_002593"
     CDS             4013..5695
                     /locus_tag="U2K71_002593"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS lactose transporter subunit IIBC"
                     /protein_id="HEM7316608.1"
                     /translation="MNTIIKQIEKGKPFFEKISRNIYLGAIRDGFLTAMPAILFSSIF
                     IMIAAVPEVFNVTLPEAFSTWLWKVYGYSMGLVGLLVVATTARCLANSMNRQLSDSGK
                     QINEVSVMLASICGFVMMAADQIDGGIATDYLGTKGLLAAFVSAFVTVNVYKFCVKRD
                     LTIRMPKEVPGTITQMFRDIIPFSIAVFIAVLIDTGVRQFFGTSFAAGVIQLLQPLFS
                     AADGYVGIAIIWGAMAMFWFVGVHGPSIVEPAIAAIIYANVETNFELFKNGAQASKVL
                     TVGLGNFVGTMGGTGATLVVPFLFMLFAKSKQLKAVGKASFIPVSFAVNEPLLFATPI
                     ILNPYFLIPFLLAPIANVWIFKLFVDVLGMNSFISVLPWATPAPIGLVLGTGFSVLAI
                     VLAVVLIVVDAIIYLPFVKAYDSELVEQELKNIAAEEVKEELVEEIQEVGATAAVIVD
                     EAKASVGEGKRVLVLCAGAGTSAMLANALEEGAKETGVNLSAKAGAYGSHYDMMKNFD
                     LIVLAPQVNSYLDDLRKDADKLGIKVIATKGAEYINLTRDPKGAVDFVLKQF"
     gene            5901..6859
                     /locus_tag="U2K71_002594"
                     /pseudo
     CDS             5901..6859
                     /locus_tag="U2K71_002594"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            6869..7105
                     /locus_tag="U2K71_002595"
                     /pseudo
     CDS             6869..7105
                     /locus_tag="U2K71_002595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016178530.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UTRA domain-containing protein"
     gene            7320..8072
                     /locus_tag="U2K71_002596"
     CDS             7320..8072
                     /locus_tag="U2K71_002596"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR transcriptional regulator"
                     /protein_id="HEM7316609.1"
                     /translation="MLKTDRFQAIMSLLEVNGTVRVSDIMEKFSVSDMTVRRDLDEME
                     KQGLLERVHGGAKLKDFYRHVELSHREKQIMLQEEKQRIVEKANEMINDGDTIFLGPG
                     TTIELMSKIIQGDSLRIVTNCLPVFEELNKRKGNRKIYLLGGEMRETTQCFVGEITNK
                     SLQDMNFHKAFFSCNALNEQKIMTSTFEEGQTQQIALNNSVERFLLIDHSKIGKRDFS
                     VYYNLNDVTVVITDDDEQKHYEKIEKEEKYVV"
BASE COUNT         2686 a         1266 c         1684 g         2640 t
ORIGIN      
        1 taacagccta agatatttgg agttcagaga gggcgcgttt gattttcaaa cttcgcaaca
       61 gaaccttcta acaattcctg gggccatgtc gcccacagag atattcaaaa tgtttgagat
      121 gggtgctgat attgttaagg tgttccccgc caactgtctt ggaccggaat atttcaatca
      181 agtgcaagcc cctttaggaa gtttaccttt gatggctgta ggaggggtag atcagactaa
      241 tgcacaaaac tatttgaaca atggagcctc ttatgtagga attggttcaa aattttttga
      301 aaaaagtgct gttcaccaac taaattacga gaggctaatg gagttggctg aatcttttat
      361 agatagttta agagttgaat agaacccatt tgcggaatac aacttgtttt ataatatggc
      421 gttatctgta taaaacatag tatcacagtg aatgagattg aactggtaga ttgatttttc
      481 gaccagttct ttttgcgcgc tctgatgctt gatatattcc caacgagata aaaaatcccc
      541 caagatacta agatgaagcg tttatccaac ctcatactta gataaaaagg aggaaatgtt
      601 ctgaatgata aacaaaaatt agcgcgcaca atttggcgat gaaaaaatca tattgtattt
      661 acgtcaaaat atcaccatca aattatttat ggaaaacata ataggcataa acccgattgc
      721 tcggctacta tgtggacaca attatagttt taaagataag tttcaattac ttattttaca
      781 cctcttcttt agggataaaa cttttcctaa cggtcaattt tgcgccgatt aatgttttct
      841 ttgatatgtc ccgcgaatat accagttgat cacttaataa atctacacag gttttagcta
      901 tctcttcgat atctatctta aaagttgtaa gtggtggtgc aacgaatttt gcgatgtcgt
      961 catcattaat acttattact gccatatctt tagggatgtt aatacccaat tcatggcagc
     1021 cctgaagaac gccggcagct atagtgtcag atggtatgaa acatgcctcg ggcatatctt
     1081 catttaactc agctttcatt ttaagagtta attgataccc ttcgtcaaca gtaaatgatc
     1141 cttgggagaa aatatacttt tcattgagta gtccgctctt ttctaaatga tatcggaagg
     1201 catattcacg gctatcaatc tcttctttac ccgtttctag gttaaagaaa tttcctccaa
     1261 taaatccaat ttttgtgaaa ccttcattaa taaagtagtt aattgcttgt ctggccattt
     1321 cgtggaaatc aggttctaca gtatcgaagt gttcaggtaa tgggtttatt cctcggatta
     1381 cccccttata ccctttttga tttaagtagt acaattcctt ttttgtcaat tgtcctattg
     1441 cgataaatcc agtaatatcg tcaggaatgc tttttacacc atcgttttta gagatgaaaa
     1501 tcaaatctag attagctttt ttactataat ttttcaaatc atctttgaca tgttgatagt
     1561 agacatcctt ttttaactgt tcctctgaga tccaaaaaag aatggcaatc ttgtacagag
     1621 tagtcttaat ttttgtcaga tcatatccta aagatttggc tgtaacgatt attttgtttt
     1681 tagtatcaga tgtaatagag agtgactcat cacctttaaa aagtcttgat atagtagccg
     1741 ttgagtaacc agattttttt gcaatatctc taatagtaac cattgttcat cctcctaggg
     1801 atataataat ccttttcttt gttaaagtaa atcgaaactg ctcttttagt aacttaaatt
     1861 actttttcta gcaggaaacg aaactctgat atcagcagag agcctaatcc attaacagag
     1921 aagattgaat cgataatgga tagccacttg gagaatgatt agtaggtggg attgatttta
     1981 gtgggaatca gtccgttttc ttgtttcgtg taaattaaat tagtaaaaag ataaatttat
     2041 tttacaaaaa aacgtttaca aaacggtttt tgaatggtat atttatttta catcaaggga
     2101 tataagtgct tgtatatcgc aataaaatag atattcgagg tcggtttttc gttgtgtaaa
     2161 aaaatttact tatacatgtt aaaattatgc tgaggtgatt tatggattat tggcatattg
     2221 atcaaactaa ttttcaagga gagattaaaa tgaattataa aactttacca gaagacttta
     2281 ttatgggagg agctacggca gcttatcaag cagaaggctc tacaaaagaa ggtggaaaag
     2341 ggcaagtagc gtgggatact ttccttgtag aaaagggacg ctatactgct gatccagcta
     2401 gtgattttta taatcaatat ccagttgatg ctgaattatg caaaaaattt aaaattgata
     2461 gtattaggat gtctattgca tggagccgta tttttccaaa aggctatggg gaagtgaacc
     2521 aaacaggagt ggaatattat cataaggtat ttgctgaatt gaataagcat ggtattattc
     2581 cttttgtaac tcttcatcat tttgataccc ctaaagaact atttgataat ggagattttt
     2641 taaatcgaga aaatgttgaa catttcgtaa actatgcaca gttctgtttc gatgaatttt
     2701 ctgaggttca ttattggagt acatttaatg agatttatcc agttgcttca aatcaatatt
     2761 tgatcggatc gtttcctcca gaagttcaat ttgatataga gaagactgta acttgtttac
     2821 acaacatgat gtatgcgcac gcaaagacag taaacatttt taaagatggt ggatataaag
     2881 gggaaattgg tgttgttcac tcgctagaga caaaatatcc agcaagtgac aaagaagaag
     2941 ataagcatgc tgcgaaatta gatgatgcgt tatcaattaa gttcttatta gatgctactt
     3001 atttagggta ttactcagat gaaacaatgg cacatattaa tgagatttta gcagcaaatg
     3061 ggcaatctgc agatattgat tctaatgatt ttagagagat gaaaaaagca gcaaatcgaa
     3121 atgattttct tggtatcaat aactatggta gccattgggt taaagagtac aaaggagaaa
     3181 gcacacttca ctacaacact accggagaaa aaggtagcca agtctattgc attaaaggga
     3241 ttggcgagag aatcgtgcgt gaggatcttc cgaaaaccga ttgggattgg atcatttatc
     3301 caaaagggtt atatgattta cttgtacgaa tccaagacga atatccaaat tataataaaa
     3361 tatacattac tgaaaacggg ctagggtata aggataattt tgaagatggg attattctag
     3421 atgatgatcg aatcgattat ttacgccagc attttaatgc gatttccgat gctatcaaaa
     3481 tgggagttaa tgtaaagggg tatttcattt ggtcattaat ggatgttttc tcttgggtta
     3541 atggatacac taagcgttat ggactattct atgtagattt cgatacacaa aaaagatatc
     3601 cgaaagagtc tgcatattgg tataaatttg ttagcgaaac aaaaacgata atataaaaat
     3661 agttaggagg gcaaggaaat ggataggaaa gaattgcaat tattaggatt tgaaattgtt
     3721 gcttatgcag gagatgccag aagttcatta ttaacagtgt tgaaggaagt gagagaaggt
     3781 aattttgata atgttgaacg attattagcg gatgctgatg aaaatttaac cttagcgcat
     3841 aactcacaaa cgaaatgttt agctgaagaa gcgtcaggga acgaaatgga agtaggcgta
     3901 atttttattc atgggcaaga ccatttaatg acaacgattc tattaagaga acttatcaat
     3961 gattttgttg aattgtacaa agaaaaattt gctaaagcat aggagaagaa aaatgaatac
     4021 gattattaag caaattgaga agggaaaacc gttttttgaa aaaatctcac gtaatattta
     4081 tttaggggca attcgcgatg gctttttaac tgctatgcca gctattctat tttcaagtat
     4141 ttttattatg attgcggctg ttccagaagt atttaacgtg acgttaccag aggcattctc
     4201 gacgtggctt tggaaagttt atggttattc aatgggatta gttggtcttt tggttgttgc
     4261 tacaacagct cgatgtttag ctaattcaat gaaccgtcaa ttatctgatt caggtaagca
     4321 gattaatgaa gtttctgtta tgttagcgtc aatttgtggt tttgtaatga tggcagctga
     4381 tcaaattgat ggtgggatag ctactgatta tttagggaca aagggattat tagcagcttt
     4441 cgtttctgcg tttgttactg taaatgtgta taaattctgt gttaaaagag atttgacgat
     4501 ccgtatgcca aaagaagttc ccggaacaat cacacagatg tttagagata ttatcccctt
     4561 ttcaattgca gtatttattg ccgttttgat tgatacggga gttcgtcaat tttttggaac
     4621 atcatttgca gcaggtgtaa ttcaattatt gcagccatta tttagtgctg ctgatggata
     4681 tgtcggtatt gcaatcattt ggggagccat ggcaatgttc tggtttgttg gtgttcatgg
     4741 accatcgatt gttgaaccag cgattgctgc aattatttat gcgaacgtag aaactaactt
     4801 tgaattattt aaaaacggtg cacaagcctc aaaagtatta acagttggtc taggaaactt
     4861 tgttggaacg atgggtggta ctggagcgac actagttgtt ccttttctgt tcatgttgtt
     4921 cgctaaatca aaacaattga aagcagttgg taaagcttca tttattccag tttcgtttgc
     4981 ggtaaatgaa ccgttactat ttgctactcc tattattttg aatccgtact ttctaattcc
     5041 atttttatta gcaccaatcg ctaacgtatg gatattcaaa ctttttgtcg atgtcttggg
     5101 gatgaatagc tttatatccg tattgccttg ggcgacgccc gcaccgattg gcttggtttt
     5161 aggaaccggg tttagtgtgt tggcgattgt attagcggta gtattgattg ttgtcgatgc
     5221 gattatttat ttaccttttg ttaaggccta tgattctgaa ttagtagaac aagagttaaa
     5281 aaacattgct gcagaagaag taaaagaaga attggtcgaa gaaatccaag aagttggtgc
     5341 aactgcggca gttatagttg atgaagcaaa agcatcggtt ggtgaaggaa agcgcgtttt
     5401 agtactttgt gctggtgccg gaacgagtgc aatgttggct aatgcattag aagagggcgc
     5461 aaaagagaca ggtgttaatc tttctgctaa agcaggagct tatggttctc attatgatat
     5521 gatgaaaaat tttgatttaa tcgttctagc acctcaagtg aattcctatt tggatgatct
     5581 ccgtaaagat gcagacaaat tagggataaa agtaattgca actaaaggtg cagaatatat
     5641 caatttaaca cgtgatccta aaggagcagt tgattttgta ttgaagcaat tttagagctt
     5701 gcttagatta ataaagcgct agccaactta gctagcgttt tatcttaaac acaatggtaa
     5761 gcgctatctt tttttgctgt ttaaatttcg ggtattcttt ttgaggcgtc gattgtaaaa
     5821 ttaagctaga caactaaaaa gatcatttgt gctacactca aaatagttcc gaccaaagaa
     5881 ttaataagga gtgagtacaa atgacctaca cccatcttac aacagacgaa ctagtaatca
     5941 tagagtctta tttcaatatc gagaaacctg tcgctcttgt cgcacaaagt ttaaatcgct
     6001 ctagacaaac catttataat gtttattcgt ttctcaaaca aggaaaaaag tgctagatta
     6061 ttaccgtcaa tacaaacaaa ataaatctcg ttgtggacga cgttcaaccg ttttatctga
     6121 tgaacaatca acatatgttc aagaaaaggt agctcaaggg tggtcacccg atgtgattat
     6181 cggccgtggc gagtttccaa ttgattgttc tgttcgtaca ctctatcgga tgtttaaaaa
     6241 aggccttttt gacacacagt cgttacctat gaaggggaaa cgtaaaccga ataatcatca
     6301 agaaaagcgt ggaaaacagg cctttcgcag aacgattcat gaacggaaca gtctctatcc
     6361 acacctgact aaagaatttg gtcatctcga aggcgacacc attgtgggag ttaaacacaa
     6421 gagcgctgtt attacattgg ttgagtgttt atccaaggtt attattactt tgaaaccgac
     6481 aggcagacga gccgaagata tcgaaaacag gttaaatgat tggttcaaat ccatgcctaa
     6541 acacttgttt aaatccatga catttgattg tggaaaagaa ttttccaact ggcgatctat
     6601 aagtaactcg aacgatattg ccatcttttt cgcagatcca ggaacacctt cgcaaagagg
     6661 gctaaatgag cactcaaacg gcttgcttag acgagatggc ttacctaaaa caatggactt
     6721 tagggacatg gatgaaacct ttattcaatc cgttgcctcg aaaagaaacc atattccaag
     6781 aaaatcatta aattatcgaa caccattgga agtatttttg agctgcatag ataaagacat
     6841 attgtctagc ttaatttgac aaatcagaaa agaaaaaatg tatcaagttt ttgaagaaat
     6901 gggttatcca attaaaacgg tcaccagaaa actagaagta atcaaagcgg atgatacgat
     6961 cagcgatttg ttagatgtgg aagaaggaga tccaatgttc tatttccata cacagggttt
     7021 ttcgctagaa cgtctgcccg ttgagtattc gatttcgaaa tatcgaggag acattaacta
     7081 ctttgttttt gaacttgcac aataacttca gattaaataa aatgatttat taaagataaa
     7141 agtcgaatga acaaggctgt cactatgaca gtcatgctcg ttcggtttta ttttttagaa
     7201 tagcagcttg caaattctga aaaattttgc tttccttcct gcagaaggct tcttaagaaa
     7261 acttgttcta gcacttttct taagaatttg atagtcttaa aaagaaaaga ggcgatgaaa
     7321 tgttaaaaac ggatcgattc caagcgatca tgtcgttatt agaggttaac ggtacagtac
     7381 gagtcagcga tattatggag aagttttcgg tttcggatat gacagtccga cgtgatttgg
     7441 acgaaatgga gaaacaagga ttattagaac gcgtccatgg cggagcgaaa ttgaaggatt
     7501 tttatcggca tgtggagctt tctcatagag aaaaacagat tatgctgcaa gaagaaaagc
     7561 agcggattgt ggaaaaggcg aatgaaatga tcaatgatgg tgatacgata tttcttggac
     7621 caggaactac aatcgagctc atgtcaaaaa tcattcaagg ggattcactg cggattgtta
     7681 caaactgttt gcctgttttt gaagaattaa ataagcgaaa agggaatcga aaaatctatc
     7741 tattaggtgg cgagatgcgg gaaacgactc aatgctttgt tggtgaaatc acgaataaat
     7801 cgctacagga tatgaatttt cataaagcat ttttcagctg taatgcttta aatgaacaga
     7861 aaattatgac ttcgactttt gaagagggcc aaactcaaca gatcgctctg aataattctg
     7921 ttgaacgctt tttattgatt gatcattcga aaattgggaa aagagatttt tcagtatatt
     7981 ataatctgaa tgatgtaaca gtagttataa cggatgatga tgaacaaaag cactatgaaa
     8041 aaatcgaaaa agaagaaaaa tatgttgtgt aaatgagaaa ctgccaaact ctagataatt
     8101 agaacttggt agttttttgg tagccttgaa tttaagcgaa ttatgttatt tttagggtgc
     8161 atcattacta attcctccga atacttgaat acaccatttg aaaaaagata gtcgattttt
     8221 ttgggatgtt atgtttatgt gaaggaaaaa atagcagtat caatcaccgc tttatt
//
LOCUS       DASHHB010000090         8202 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.094, whole genome shotgun sequence.
ACCESSION   DASHHB010000090 DASHHB010000000
VERSION     DASHHB010000090.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.094"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(25..162)
                     /locus_tag="U2K71_002597"
     CDS             complement(25..162)
                     /locus_tag="U2K71_002597"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3789 domain-containing protein"
                     /protein_id="HEM7316610.1"
                     /translation="MKRGIVMGIVKDIFLVFGGSMLGVTVMCLMNASAAADRAMEKKG
                     K"
     gene            complement(159..1343)
                     /locus_tag="U2K71_002598"
     CDS             complement(159..1343)
                     /locus_tag="U2K71_002598"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEM7316611.1"
                     /translation="MAQRNLDYRLLKDRRNEYGVSQNKLATTCGLSRPYLNQIENGGV
                     TASTKTMRKIFDQLESFNPELPLTLLFDYVRIRFPTTDARKIIQEILHLKFDYMLHED
                     YAFYSYQEQYVMGDIVVMLSHEEDKGVLLELKGRGCRQFETFLLAQKRSWYDFFEDCL
                     KAGGVMKRLDLAINDRVGLLDIPELTKKCQKEECISLFRTFKSYRSGELLKADEKDGM
                     GNTLYIGSLKSEVYFCLYEKDYEQYIKLGIPLDQTETKNRFEIRLKNDRAFHAIQDLL
                     KGRSIESTTFSIINRYLRFADKVEGKRRTNWPLNEQWGRFIGRNRKEIQLTSEPKPYT
                     IERTLNWLGRQVAPTWKMAKELDRLNRTTYIQDMVQNARLSDRHKKILEQQSMAIENL
                     II"
     gene            complement(1599..1853)
                     /locus_tag="U2K71_002599"
     CDS             complement(1599..1853)
                     /locus_tag="U2K71_002599"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316612.1"
                     /translation="MIGKIQSDPELEEELYRLNFDIRKNGVKVPDDPYVIDEETDARM
                     ELNQVISELERIADFAKEPDIRQYLFEIKAELEIEGITAE"
     gene            complement(1878..3020)
                     /locus_tag="U2K71_002600"
     CDS             complement(1878..3020)
                     /locus_tag="U2K71_002600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HEM7316613.1"
                     /translation="MTFLDLFAGIGGFCLGMEQAGHQCIGFCEIDDFARQSYKAIHDT
                     SKEVEMHDITSVSDEFIQSLGPVDILCGGFPCQAFSIAGKRQGFSDTRGTLFFEIARF
                     AAILQPKFLFLENVRGLLNHEGGATFETILRTLDGLGYDVEWQVLNSKAYVPQNRERI
                     FLIGHSRDACTEQVFPIIGSSPTSDQNIRNLLNINPSNRGMGGQVYGSDDVAPTLTGD
                     EGIKIALPVDDGISVAGMLLGNFEQGNRVYEITGTAPTLSTKQGGTKIMLRKNETEYQ
                     EVKPGNSVNLAFPNSTSRRGRLGKQSVHTLLTGDQQAVVTDQYQIRKLTPRECWRLQG
                     FPDWAFDRASQVNSDSQLYKQAGNSVTVPVIFDIARRLKEVKTDDL"
     gene            complement(3080..4432)
                     /locus_tag="U2K71_002601"
     CDS             complement(3080..4432)
                     /locus_tag="U2K71_002601"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HEM7316614.1"
                     /translation="MYKGHRIRAGDQHLVYHFVLGWLLALFIGWMSVFYFQELRQFDI
                     SKLSLSTIEIVRSIKDLIYLLGSLVLSGSTMLLYIHFFQDHWRSLWHRQKLARMILEN
                     HWYEVKQTQSEGFFKDLNSSRTKETISYFPKIYYRMKDGLLSIRVQISLGKYQDQLLK
                     LEKKLESGLYCELVEKELKDSYVEYTLLYDIIANRIGIDEVVAESGALRLMKNQVWAY
                     DSLPHMLIAGGTGGGKTYFLLTIIEALLKSDAELFVLDPKNADLADLGTVMPHVYSQK
                     EEISACVEDFYERMMTRSKAMKEMPNYKTGENYAFLGLPPNFLIFDEYVVYMEMLTTK
                     ESAVILNKLKQIVMLGRQSGFFLILACQRPDAKYLGDGIRDQFNFRVALGRMSELGYS
                     MMFGEVDKKFFMKRIKGRGYVDTGGSVISEFYTPLVPKGYDFLREISDIGGLNVNTEG
                     "
     gene            complement(4503..4847)
                     /locus_tag="U2K71_002602"
     CDS             complement(4503..4847)
                     /locus_tag="U2K71_002602"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelB/DinJ family
                     antitoxin"
                     /protein_id="HEM7316615.1"
                     /translation="MEINVEHILDCLDQYGKGELTEEQLTKALTYDEKMFLIMHQGLL
                     EVGNDVEEDFDVLNWLGEEESFFMVIEVNESLCREAETVLEEIGVEMPDAIEVFLKQL
                     VETKQLPIAVND"
     gene            complement(4857..5231)
                     /locus_tag="U2K71_002603"
     CDS             complement(4857..5231)
                     /locus_tag="U2K71_002603"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM7316616.1"
                     /translation="MRLTEGIVVDSGLTFGKLRFSALRREVRKQNEDGTVSNEVKERT
                     YNLKSSAQGRMIQVSIPANVPLREFAYDAEVELVNPIMDTVANYVFREGTTVNWFIKA
                     DDLVLKRQPDQGNSTNQNEGKK"
     gene            complement(5244..5558)
                     /locus_tag="U2K71_002604"
     CDS             complement(5244..5558)
                     /locus_tag="U2K71_002604"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016171806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcP family protein"
                     /protein_id="HEM7316617.1"
                     /translation="MELKFVVPDMAETFGKIRYAGEGEVLTEGYGRNTTVIGRSYHLY
                     SSKQRADDIEVVVAAEAGEKDFDQDQPLKAVNPHLVAKGYEIENRGFTDYVLYVDDLV
                     KA"
     gene            complement(5672..>8202)
                     /gene="fss3"
                     /locus_tag="U2K71_002605"
     CDS             complement(5672..>8202)
                     /gene="fss3"
                     /locus_tag="U2K71_002605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="fibrinogen-binding MSCRAMM adhesin Fss3"
                     /protein_id="HEM7316618.1"
                     /translation="GELTLKKSANTGTPVAYKMAGQQTLMAGAIDKPNTYTIKIDVET
                     EGSLKIKKVDKETGNVVPGTVFHLDFGKTLPAKDVTTDKEGIATLDEIPHGAKVTITE
                     KSVPAPYTIDTTPITATIKSGETISVTSKNTREKGQIILNKMGVETGTDLWNDKYSLA
                     GNTFAIRKDSPTGEIVQEMTTDEKGHAETPKEIANALELGTYYVTETKASNGFVNTFK
                     PVKVELNYVNQTVALVTSNVKGQNQEITGETTLTKEDKDTGNETQGKAEFKGAEYTLF
                     TAKDGQAVKWSEAFKPELVKGTKASDETVTLTLDEKNQVAVKHLAINEYYWQETKAPE
                     GYTLDETKYPVSIKKVDDNEKNAVITRDVTAKEQVIRFGFDFFKFAGSAAGTAETGFN
                     DLTFKVSPLEGTSEITGAEDEAITAYNEQLGFDGYGKFENLPYGDYLLEEVEAPEGFQ
                     KITPLEIRSTFKENKADYTKSEYIFTITEKGQKQPIKTVTVPYEKLTNKAFSVSLNRL
                     MLYDLPEEEDSLTSLGTWKDGGKELTDLDSTELVDKLSYNLHEIKEDWYVVAQAIDVE
                     ATKAAQEKDEKAKPVVIAETTATLANKEKTGTWKILHKLTAEQVLDKTIVLFNYVYEN
                     KKPYEAGDDPVAKDASLNNQAQTVNCTVERHVSIQTKAHLEDGSQTFTHGDVVDMFDD
                     VSITHDVLDGSKEAFETILYALLPDNTTKEIWKSGKIDYEVNDKEFTKTVLAEKVDTS
                     KYPEGTSFTFKEINYGKDGNINGKHNEDLKEKTQTLTPKKVPTTPSAPEQPEIPTVPS
                     DSQESSPTVKTFPQTGEKNSNVLLFIGFTLIFATAGYYFWNRRN"
BASE COUNT         2182 a         1916 c         1410 g         2694 t
ORIGIN      
        1 aaattttgtc cgaaattcat cttgtcactt tcctttcttt tccattgctc gatccgctgc
       61 tgcacttgca ttcatcaagc acatcacagt aacaccaagc atactgccac caaataccaa
      121 aaaaatatct tttacaattc ccatgacgat tcctcttttc atataatcaa attttcaatt
      181 gccatacttt gttgctccaa aatcttttta tgtcggtctg ataagcgtgc attctgtacc
      241 atatcctgga tataggttgt ccgattcaat cgatccaact ccttagccat tttccacgta
      301 ggggcgactt gtcggcctag ccaattcaag gttcgttcaa tggtatacgg ttttggctcg
      361 gaagttaatt gaatctcttt ccgattccgt ccaataaaac gcccccattg ttcattcaag
      421 ggccagttgg ttcgcctttt accttctact ttatccgcaa atcgcaagta acgattgata
      481 atggaaaacg tggtgctctc aatgctacga ccttttaaca aatcttgaat cgcatgaaaa
      541 gcacggtcat ttttcagtcg aatctcaaaa cgatttttcg tctcagtttg gtctaatgga
      601 atccctaact tgatatattg ttcatagtct ttttcataca aacagaaata aacctcactc
      661 ttgagactgc cgatatataa ggtattgccc atgccgtcct tttcatcagc ctttaaaagt
      721 tccccggaac gataactttt aaaggtacga aacaaggaga tacattcttc cttctgacat
      781 tttttcgtta gctcaggtat atccaatagc cccactcgat cattgattgc caagtccaag
      841 cgtttcatca cgccaccggc ctttagacaa tcctcgaaaa agtcgtacca gctacgcttt
      901 tgagcgagta aaaaagtttc aaattgtcgg caacctcgcc cctttaattc taaaaggacg
      961 cctttatctt cttcatgtga caacatcacc acaatatctc ccataacata ttgttcttga
     1021 taggagtaaa aggcgtaatc ttcgtggagc atataatcaa atttcaaatg gagaatctct
     1081 tgaatgattt tcctcgcatc cgttgtggga aagcgaatcc ttacatagtc aaacagtaac
     1141 gttaaaggta actcgggatt gaaactttcc agttggtcaa aaattttcct catggttttt
     1201 gtcgatgcgg ttacgccacc attttctatc tgatttaagt acgggcgact aagtccacaa
     1261 gtcgtagcta atttattttg tgaaacgcca tattcattac gccggtcttt taatagccga
     1321 taatctaaat tcctttgtgc caagttctca cctccaaatt taaggtgtta actttttcaa
     1381 atcagttaac atctacacaa attgcttatt atccttgata ggacagccat tccttatcgt
     1441 tgcttgctta ttttttgtgt ttttgtaccc ccctgttaga tacggggggt gagacatcat
     1501 ggccggctaa cgccgtccat aaacaggcac agcaccacgg aggatttctg cttcgcaaat
     1561 cccccgcggt cagtgcctat cagtggttta tttggccgtt actccgcggt aataccctca
     1621 atttccaact ctgcttttat ctcaaacaag tattggcgga tatcaggttc tttagcaaag
     1681 tccgcaattc gttccaattc actgatgact tgattcagct ccattcgagc atccgtttcc
     1741 tcatcaatta catacggatc atcagggact ttcaccccgt ttttgcgaat atcaaaattg
     1801 agccggtaca gttcttcttc caattccgga tctgactgaa tcttgccaat cacccagtag
     1861 attccccaat caactcatca taaatcatct gtttttacct cctttaatct tctagcaata
     1921 tcaaaaatga ccggaactgt tacagaattt ccagcctgct tatacaactg actatctgaa
     1981 ttgacttgag aagctcgatc aaaagcccag tccggaaagc cctgaagccg ccagcactcc
     2041 ctaggtgtta actttcgaat ttgatactga tccgtgacga ctgcttgttg gtctcctgtt
     2101 aataaggtat gcacactttg ttttcccaga cggcctcgac gacttgtgga attaggaaaa
     2161 gccaggttca cactgttccc tggcttcacc tcttgatatt ctgtttcgtt tttccgaagc
     2221 atgatcttcg ttccgccttg tttcgttgac aaagtaggcg ctgttccagt tatctcataa
     2281 acccgattgc cttgttcaaa atttcccagt aacatacccg ctacagaaat tccatcatct
     2341 accggcaatg cgattttgat tccttcatca ccggtcaagg taggcgctac gtcatctgac
     2401 ccatagactt gtccccccat tcctcgattg gaagggttga tatttaataa gtttctgatg
     2461 ttttgatcag atgttggaga cgatccaatg ataggaaata cttgttcggt acatgcgtct
     2521 cgagaatgtc cgataaggaa gatccgctca cggttttggg gaacgtaggc ctttgagtta
     2581 agcacttgcc attccacatc ataccccaat ccatccagcg ttcggaggat cgtctcgaac
     2641 gtagcccctc cttcgtgatt gagtaatccc cggacgttct caaggaataa aaacttaggt
     2701 tggagaatag cggcgaaccg agcgatttca aagaataaag tacctcgagt atcggaaaat
     2761 ccttgccgct ttcccgcaat tgaaaaagct tggcacggaa atccaccgca aaggatatcc
     2821 actggtccga gagattgaat aaactcgtct gatacacttg tgatgtcatg catttccacc
     2881 tccttacttg tatcatggat tgccttataa ctctggcgag caaaatcatc aatttcacaa
     2941 aagccgatac actggtgacc cgcttgttcc atacctaagc aaaagccacc aatcccagcg
     3001 aataaatcta aaaatgtcat aaatcacctc gtaaaaaaag cccaggattc ttgacactcc
     3061 taggctacat actattattt tatccttccg tattaacgtt taatccacca atgtcgctaa
     3121 tttcccttaa gaaatcatat ccttttggta caagtggcgt ataaaactca ctaatcacac
     3181 tccctcccgt atctacatag cctcgacctt tgatacgctt cataaagaat tttttatcaa
     3241 cctcgccaaa catcatggaa tatcccagtt cactcatacg acccaaagcc acccggaaat
     3301 taaattgatc gcgaatcccg tctcctaaat atttcgcatc cggtctttga caagctagaa
     3361 tcagaaagaa accagattga cgacctaaca tcacgatttg tttgagctta ttcaaaatca
     3421 ccgcactttc cttcgtcgtt aacatttcca tataaaccac gtattcatca aagattaaaa
     3481 agtttggtgg aagtccaaga aacgcataat tctctcctgt tttgtagttg ggcatttctt
     3541 tcattgcctt gctacgagtc atcatgcgct catagaaatc ttccacacaa gcagaaattt
     3601 cctccttttg ggaatagaca tgaggcatta ccgtacctaa atcggctaag tccgcatttt
     3661 tgggatcaag aacaaaaagc tcggcatcag attttaataa agcctcgatg atagtgagta
     3721 aaaagtaagt cttcccacca cctgtgccgc cagcgattaa catatgaggt aaagaatcat
     3781 aagcccatac ttgatttttc atcaatcgca aagccccact ttctgccact acttcgtcta
     3841 tcccaattcg attggcaatt atatcataca gtaaagtgta ctccacatag gagtctttga
     3901 gttccttttc tactagttca caatatagac cactttctaa cttcttttcc agctttaaaa
     3961 gctgatcttg atattttccc agtgaaattt gaacgcgaat agaaagtaaa ccatccttca
     4021 tacggtaata gattttgggg aagtaactga ttgtctcttt ggttcgacta ctattgaggt
     4081 ctttgaaaaa tccttcactc tgagtttgtt ttacttcgta ccaatgattt tctagaatca
     4141 tccgggctag cttttgtcga tgccacaaac tgcgccaatg atcctgaaaa aagtgtatat
     4201 aaagcaacat cgtgcttcct gaaagaacga gacttcctag caaatagatt aaatctttta
     4261 tagaacgaac gatttctata gtagaaagtg atagcttaga aatatcaaat tgtctaagct
     4321 cttgaaaata aaagacgctc atccaaccga tgaacagagc gagtagccag ccaagaacaa
     4381 agtgataaac caagtgctga tctcctgccc gtatacgatg ccctttgtac atttttatgg
     4441 tttccacctc caatcgaaaa aagcttcata ggatttcatt gatccaattt caagcagtta
     4501 tgctagtcat ttactgctat agggagttgt ttcgtttcca ctagttgctt taaaaaaact
     4561 tcaatggcat ccggcatttc cacaccaatt tcttccaata cggtttcggc ttctcgacat
     4621 agactttcat ttacttcgat aaccataaag aaggactctt cttcccctaa ccaattcagt
     4681 acatcaaaat cttcttcgac atcgttacct acttccagta acccttgatg cataatcaga
     4741 aacatttttt catcataggt taatgcttta gtgagttgtt cctccgttag ctcacctttt
     4801 ccatattgat cgagacaatc taaaatatgt tcaacattaa tttccatacg cttcacctat
     4861 ttctttcctt cattttggtt tgtagaattt ccctgatctg gttgtcgttt caagactaaa
     4921 tcatcggcct taatgaacca attgacagta gttccttctc ggaaaacata attggcgacc
     4981 gtatccatga ttggattgac caactctacc tctgcgtcat aggcaaactc gcgtaagggt
     5041 acatttgctg ggatactaac ttggatcatt cgaccttgcg cagaggattt caaattataa
     5101 gtccgttctt ttacctcgtt actaaccgtt ccatcctcat tttgcttccg tacttctcgg
     5161 cgtaatgcgg aaaatcgtaa tttcccaaac gttagtcctg aatctacaac aataccttct
     5221 gttaatctca tcttcttttc ctcctatgct ttcactaaat catcgacata caacacgtag
     5281 tcggtaaacc cacgattctc aatctcatag cctttggcaa ccaaatgggg attcacggct
     5341 tttagtggtt gatcttgatc aaaatccttt tcgccggctt ccgcagctac caccacttca
     5401 atatcatcgg ctcgttgctt actggaatac aaatgataac tacgaccaat tactgtagta
     5461 ttccgcccat atccttcagt taaaacttcg ccttcgccgg cataccttat ctttccaaag
     5521 gtttcggcca tatctggcac aacaaatttc aattccatct tcttttcctc ttttcatttt
     5581 tagttttgga tacaaaaata gcgcgaccac tttttttagg aggcggtcgc gcacgttggt
     5641 ttttcctatt tagttgtttc atcacatcac cttagtttcg gcgattccag aaataatacc
     5701 cagcagtcgc aaagatcaag gtaaacccaa taaacagcag aacattagaa tttttctccc
     5761 ctgtttgtgg gaatgtcttt actgtaggac tagattcttg agagtcactt ggaacagttg
     5821 gtatttccgg ttgttctggt gcacttggtg ttgtcggaac ttttttaggt gttaaagttt
     5881 gagttttttc ttttagatcc tcattgtgtt tcccattgat gtttccgtct ttgccatagt
     5941 taatttcttt gaaggtaaaa cttgtacctt ctggatattt acttgtgtcc actttttccg
     6001 caagtacggt tttagtgaat tctttgtcat tcacttcata atcaattttg ccagatttcc
     6061 aaatttcttt ggtcgtatta tccggaagta atgcatacag aattgtttca aaagcttctt
     6121 ttgagccatc cagtacatca tgggtaatcg acacatcatc gaacatatcc accacatccc
     6181 catgagtaaa ggtttgcgaa ccatcttcta ggtgggcttt tgtttggatg gaaacatggc
     6241 gttccaccgt acaattgaca gtttgggctt gattgttcaa gctagcatct ttcgctactg
     6301 gatcatcacc tgcttcatag ggtttcttgt tttcatacac atagttgaat aagacgatgg
     6361 ttttatccaa aacttgttct gcggttaatt tatgcaaaat tttccaagtt cctgttttct
     6421 ctttattcgc caacgttgcg gttgtttcgg caattaccac tggtttggct ttttcatctt
     6481 tttcttgggc agcttttgtc gcttccacat caatggcttg cgctaccaca taccagtctt
     6541 ctttgatttc atgcaagtta tagctcaatt tatcaactag ctcggtagaa tcaaggtctg
     6601 tcaattcttt gcctccgtct ttccaagtcc caagagaagt taagctatct tcttcttcag
     6661 gtaaatcata gagcatcaaa cgattcaagc taacagaaaa tgctttattc gtcagtttct
     6721 cgtaaggaac ggtcaccgtt ttaattggtt gtttttgtcc tttttcggta atcgtaaaaa
     6781 tgtattcact cttagtgtaa tccgctttat tttctttaaa agtagaacgg atttctaatg
     6841 gcgtaatttt ttgaaatcct tctggagcct ctacttcttc aagtaaataa tccccataag
     6901 gaagattttc aaacttgcca tagccatcaa aacctagttg ctcgttataa gctgtaattg
     6961 cttcatcttc ggcacctgta atctcactgg ttccttccaa tggcgatact ttaaaggtca
     7021 gatcattgaa gccagtttcg gcagtcccag cagctgatcc agcaaattta aagaaatcaa
     7081 agccaaagcg aatgacttgt tcttttgccg taacatctcg ggtaatcacg gcattttttt
     7141 cgttatcatc cacctttttg atggaaacag ggtactttgt ttcatccaag gtatatcctt
     7201 caggtgcttt ggtttcttgc cagtaatact cattaatcgc taagtgttta acagcaactt
     7261 gattcttttc atctaaagtc aaagtcaccg tttcatcaga agctttcgtt cccttcacta
     7321 actctggttt aaaagcctca ctccatttga ccgcttgacc atcttttgca gtaaagagag
     7381 tatattcagc tcctttaaac tcagctttac cttgagtttc attaccggta tctttgtctt
     7441 cttttgtcaa agtggtttca ccagtgattt cttggttttg cccttttacg ttactggtaa
     7501 caagagccac ggtttgattg acatagttca attcgacttt tactggttta aaggtattca
     7561 caaagccatt actagctttg gtttcggtca cataataggt tcccaattcc aacgcattgg
     7621 caatctcttt tggtgtttcc gcatgacctt tttcatccgt ggtcatttct tggacaattt
     7681 caccggttgg actgtctttg cgaatggcaa acgtatttcc ggctaatgag tatttgtcgt
     7741 tccaaagatc agttcctgtt tccaccccca ttttattcaa aatgatttga cctttttcgc
     7801 gagtattttt tgaagtcaca gaaatagtct caccagattt aatggttgcg gtgattggtg
     7861 tagtgtcaat cgtataagga gctggcactg atttttcagt aatggttact ttcgcgccat
     7921 gaggaatctc atccaatgtc gcaatgcctt ctttatcggt ggtcacgtct tttgcaggta
     7981 atgtttttcc aaagtctaaa tggaaaacgg ttcccggtac aacatttcct gtttctttat
     8041 cgactttttt aatcttcaaa gaaccctcag tttccacatc aattttgatt gtgtaggtat
     8101 tgggcttatc aattgccccc gccatcaaag tttgttgacc agccatttta taagctaccg
     8161 gtgttcctgt attagctgac ttctttaggg ttaattcacc ac
//
LOCUS       DASHHB010000091         8066 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.095, whole genome shotgun sequence.
ACCESSION   DASHHB010000091 DASHHB010000000
VERSION     DASHHB010000091.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 8066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.095"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..883
                     /locus_tag="U2K71_002606"
     CDS             <1..883
                     /locus_tag="U2K71_002606"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002320632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEM7316619.1"
                     /translation="ISVPVALFSFMGSIMAGIFANIVNFKQYQTIYAGFNPAAESYTY
                     NDYFQIGMIGMIVSLVVVLTVANISMNKKKRYAMAANVPAEGGDAPMISWLAVLLPVL
                     GVVLLDLQIILGFILAGIWALLFTGKLRGGYKEICRQFAKLFTDGAVDVAPMVGFLMT
                     LAMFNNSAAYASPYFSAIFGDWIPQSPLVLAIVFAILTPLGFFRGPMNLVGSGSAILA
                     VVLAVNPTMSPAFLFPLFAITTIAPQHLDITQSWVAWGLGYTKVTSREYMKKSIPTGW
                     IIGAILCLITFLLYGNA"
     gene            931..3060
                     /locus_tag="U2K71_002607"
     CDS             931..3060
                     /locus_tag="U2K71_002607"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HEM7316620.1"
                     /translation="MKRFVRMGIDVGGTHTKAVAIDNATHEIIGKSSVKTTHDDVRGV
                     AAGVVQSFQNCLRENNISPEDVVFVAHSTTQATNALIEGDVAKVGVIGMAKGGLEGFL
                     AKRQTRLNDIDLGNKKKIEIVNAFLPVKNLNVDRVSETISSLERERAEVLVSSMAFGV
                     DNGEPERVVYEAASVKAIPTTMASDITKLYGLTRRTRTAAINASILPKMLDTATSTED
                     SVREAGVNVSLMIMRGDGGVMEINEMKKRPVLTMLSGPAASVMGSLMYLRASNGVYFE
                     VGGTTTNIGVIKNGRPAIDYSIVGGHPTYISSLDVRVLGVAGGSMVRANQSGIIDVGP
                     RSAHIAGLDYAVFTETEKIKGPKVEFFSPKEGDPADYVKVVMEDGEEVTITNTCAANV
                     LGLVQEEHFSYGNVPSARKAIQALADYCHTTVEDIAEQIMEKSYAKIEPVILELADKY
                     HLEKDQISLVGVGGGAASLITYFSNKMGVKYSIPENAEVISSIGVALAMVRDVVERII
                     PSPSKEDIRSLKNEAMNKAIESGATPESIEVHVEIDPHTSKVTAIATGSTEVKATDLT
                     KEITTEEALELAAEDMRLNKNEVCLLENTPFFYVCGEQNRSKNAGSLRIIDQKGFIKV
                     QRGHASCMKTTAANYMTAVEQLWEDMAVYQTELIARPEFYLCLGARVSDFTATDLEQL
                     QLLMDLEVSTMEPEEEVIVVAGNIKQT"
     gene            3035..3724
                     /locus_tag="U2K71_002608"
     CDS             3035..3724
                     /locus_tag="U2K71_002608"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002307432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316621.1"
                     /translation="MPEISNKHKLEELLNELQQIPEEIWGFYQFRRDLFWKKIPLSKQ
                     KILIQQSIDCGIETACSIKKKYPFADVGEICEQMAVPIVSCESEQINERITFATYAED
                     EGIRLMTEPLEKLKCSGLASISKETAQALIIGHELFHHIEASVKGIYTQSEKIVLWRL
                     PFYTHQSNIRALSEIAAMSFSKEMNQSDFSPYVLEAVLLWPYNEIRSQGILEEIKEIE
                     KRCTEYDLDYK"
     gene            complement(4132..4818)
                     /locus_tag="U2K71_002609"
     CDS             complement(4132..4818)
                     /locus_tag="U2K71_002609"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002374310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM7316622.1"
                     /translation="MVINNLQQQAYESIRKQIIYSELAPGSKISDKVLEEQLAIGRTP
                     IREALIQLRNQELVKTIPQSGTYVSKIDIRSASLARYTREKLEKPILQECSAKMTKQD
                     QAKLEKILEQTDQAVIAQNKKAFFDLDTDFHRTCYEIAGKREIWDWLESYSTHLNRFR
                     WLRLTISELDWGRVLDEHQTMLQSMIDHNFDEVGFLCTMHLHMIIEEQEYVIHNYPDY
                     FEDIQDRPIK"
     gene            complement(4954..5148)
                     /locus_tag="U2K71_002610"
     CDS             complement(4954..5148)
                     /locus_tag="U2K71_002610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316623.1"
                     /translation="MKRKKQVIPMYVVKVMHGYIDKTGCRTREKNLDNLLIFKDKKES
                     EAFAKRIGGRVKPIQEVRPD"
     gene            complement(5198..5638)
                     /locus_tag="U2K71_002611"
     CDS             complement(5198..5638)
                     /locus_tag="U2K71_002611"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HEM7316624.1"
                     /translation="MEKFTGYVREPFYYETDQMGIIHHSNYIRWFEEARVALLVHLGF
                     PYKKIEESGIIIPVLEVNCSYKEMIHYGEKVSIHPTIQKYTGTRLDFSYEIVGTEDKK
                     LKTTGSSKHCFLLSDSQRLVKLSKVNPELEQLFQAYASLDDVNS"
     gene            complement(5642..6487)
                     /locus_tag="U2K71_002612"
     CDS             complement(5642..6487)
                     /locus_tag="U2K71_002612"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008264125.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate dehydratase"
                     /protein_id="HEM7316625.1"
                     /translation="MLINEQLVKRVITPRPKISHKGTFGRIVLIGGNEQYGGAIIMSA
                     LAAVHSGAGLTTVVTDEKNRSALHSHLPEAMIVDWTDTDQFLSVIKQADVLLIGPGLG
                     TSSKSLERFKQVLENQSKEQWLVIDGSALSLLVANHLLLPYPEQTVLTPHQMEWQRLS
                     SLPIQEQRDKENKKKQQEIGAVLVLKSHETKIYGHQHFYTNPLGSAAMATGGMGDTLA
                     GMITGFLAQFPQNDQTIAAAVYLHSFIGDQLAKEQYIVLPTQISEALPKWMNYFCKKS
                     MENEA"
     gene            6636..8054
                     /gene="pepV"
                     /locus_tag="U2K71_002613"
     CDS             6636..8054
                     /gene="pepV"
                     /locus_tag="U2K71_002613"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019723407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptidase PepV"
                     /protein_id="HEM7316626.1"
                     /translation="MTIDWQKEVEARKEDLLEDLKNLLRVNSERDDSKVTPDAPFGPG
                     PRDALKHMLAYGERDGFKVKNVDNYAGHIDLGEGDETLGIFGHMDVVPAGDGWDTDPY
                     EPVIKDGKIFARGSSDDKGPSMAAYYAMKIIKELDLKLSKKVRFVVGSDEESGWGDMA
                     YYFEHEEEPDFGFSPDAEFPIINGEKGNVSLALRFKGDNAGDYVLKSFVSGLRENMVP
                     GTATAALQVPSADAAIAMEEAFYQFIEANPVSGTIEADNTYVKIELVGKGAHGASPQS
                     GINAGSFLALFLDNYEFLGSAKQFIHVAAAYVHEDFYGEKLGVAYEDEKMGKLTMNAG
                     LFAFEENGTEEANFINMNFRFPKGVTVDGLQSDIEQTVGQEGATVTRGARVMEPHYVP
                     MDDPLVATLLQVYEDHTGEKGYEQIIGGGTYGRLLKRGVAYGAMFPGYTDTMHQANEF
                     MSLDDLFRATAIYADAIYRLAK"
BASE COUNT         2480 a         1515 c         1713 g         2358 t
ORIGIN      
        1 tatttcagta ccggttgcac tgttctcatt tatgggctcg attatggctg gaatctttgc
       61 taatatcgtc aatttcaaac aatatcaaac gatttatgct ggtttcaatc cagcggctga
      121 aagctatact tataatgact atttccaaat tggtatgatt gggatgattg taagcttagt
      181 tgtggtacta acggtcgcta atatatcaat gaacaaaaag aaacgctatg caatggcagc
      241 aaacgtacca gctgaaggtg gcgacgcacc aatgatctca tggttagccg tattattacc
      301 agttttaggg gtcgttcttt tagatcttca aatcatctta ggttttattc tagcaggtat
      361 ctgggcattg ctgtttactg gaaaattgcg cggaggttat aaagaaatct gtcgtcagtt
      421 tgcgaaatta tttacagatg gcgcagtcga tgttgcacca atggttggtt tcttgatgac
      481 cttagcgatg ttcaataatt cagctgcgta tgcttctcct tatttttcag ctatttttgg
      541 agattggata ccgcaatcac cattagtctt ggcaatcgta tttgctatat tgacaccact
      601 aggatttttc cgcggtccaa tgaatctagt cggttcaggc tcagctattt tagctgtcgt
      661 gttagctgtt aatccaacga tgtcgccagc ctttttgttc ccgttatttg caattacaac
      721 gattgctcct cagcatttag atattacaca atcttgggtt gcttggggat taggttatac
      781 aaaagtaact tctcgagaat atatgaagaa atccatccca actggttgga tcatcggtgc
      841 gatcttatgc ctgatcacgt tcttattgta cgggaacgcc tagtaaacaa aacgtcgata
      901 aaaacgatta gataaatgga ggaaaagaaa atgaaacgtt ttgtgcgaat gggaattgat
      961 gttggcggta cacatacgaa agcagtcgca atcgataatg cgacgcacga aatcatcgga
     1021 aaatcttcag tgaaaacaac ccacgatgat gtccgtggag tagctgcagg tgtcgttcag
     1081 tcttttcaaa attgtttgcg ggaaaataat atcagtccag aagatgttgt gtttgttgca
     1141 cattctacca cacaagcgac taatgcactg attgaaggag acgtagcaaa agtcggcgta
     1201 atcgggatgg caaaaggtgg attagaaggt tttttagcaa aacgacaaac tagattgaac
     1261 gatatcgatt tagggaataa gaaaaaaata gagattgtca atgcattctt acctgtcaaa
     1321 aacttaaatg tagaccgtgt ttcagaaacg atttcttctt tagaaagaga aagagcggag
     1381 gtcttggttt cttctatggc atttggagta gataatgggg aaccagaacg agttgtttat
     1441 gaagcagcat ctgtgaaagc gatccctaca acgatggctt ctgacatcac aaaattatat
     1501 ggattgacaa gaagaactcg gacagcagca atcaacgcat ctattttacc aaaaatgcta
     1561 gatacggcaa cctcgacaga agactccgta cgagaagcag gggtcaatgt ttctttgatg
     1621 attatgcgcg gagacggcgg agtaatggaa atcaatgaaa tgaaaaaacg acctgtttta
     1681 accatgcttt ccggtcctgc tgcatcagtc atgggctctt tgatgtactt gagagcgtct
     1741 aatggtgttt attttgaagt aggcggaaca acaaccaata tcggggtcat taaaaatgga
     1801 cgtccagcaa tcgattattc aattgtaggt ggccacccaa catatatctc ttcattagat
     1861 gttcgggttc taggtgtggc aggcggttcg atggtacggg ctaatcagtc aggaatcatc
     1921 gatgtcggtc cacgttctgc acatattgcc ggtctagatt atgcagtatt tacggagaca
     1981 gaaaaaatca aaggtcctaa ggttgaattt ttctctccta aagaaggaga ccctgctgat
     2041 tacgtcaagg tagtgatgga agacggagaa gaagtgacta ttacaaatac atgtgcagcc
     2101 aatgttcttg gattagtgca agaagaacat ttttcttatg gaaatgtccc atctgcacga
     2161 aaagccatac aggcattagc agactattgt catacgacgg ttgaagacat cgcagagcag
     2221 atcatggaaa aatcctatgc taagattgaa ccggtgattt tagaattggc agacaagtat
     2281 cacttggaga aagatcagat ttctcttgta ggtgtagggg gcggcgctgc ctcattgatt
     2341 acttacttta gtaataaaat gggtgtgaaa tattcgattc ctgaaaacgc cgaagtgatt
     2401 tcttctattg gtgtagcact tgcaatggtc cgagatgttg tagaacgtat catcccttcg
     2461 ccaagtaaag aagatatacg atcgttgaaa aatgaagcga tgaataaagc gattgaatca
     2521 ggcgctacac cagaatcgat cgaagtccac gtagaaatcg atccacatac ttcaaaagta
     2581 acggctatcg ctactggttc tacggaagtg aaagcaaccg atctaaccaa ggagatcaca
     2641 acagaagaag cactagagct tgctgcagag gatatgcgat taaataaaaa tgaagtttgc
     2701 ttattggaaa atacaccttt cttttatgta tgcggcgaac agaatcgttc aaaaaatgct
     2761 ggaagtcttc ggatcatcga tcaaaaagga tttatcaagg tccaacgtgg acatgcttca
     2821 tgtatgaaaa caactgcagc caattatatg acagctgtcg aacaactttg ggaagatatg
     2881 gcagtttatc aaactgaatt gattgcccga ccagaatttt atctctgctt aggagcaaga
     2941 gtctctgatt ttacagcgac ggatctggaa caactacagc ttttgatgga tctggaagta
     3001 tctactatgg aaccggagga agaagtgatc gtcgttgccg gaaatatcaa acaaacataa
     3061 gttagaagag ttactaaacg aattacaaca gatccccgaa gaaatttggg gattctacca
     3121 gtttcggcga gatttatttt ggaaaaagat tcctttgtcc aagcagaaaa ttcttataca
     3181 acaatcaatc gattgtggta tcgaaacggc atgtagcatc aaaaaaaagt acccttttgc
     3241 cgatgtagga gaaatctgtg aacaaatggc tgttccaatc gtttcctgtg aatcagagca
     3301 aataaacgaa cgaatcactt ttgcaactta tgcagaagat gaagggatcc gattgatgac
     3361 ggagccgtta gaaaaattaa aatgttcagg actagcttcg atctctaaag aaacagcgca
     3421 agctttgatt attggacatg aactatttca ccacatcgaa gcttctgtta agggaattta
     3481 tacgcaaagc gaaaaaattg tgctgtggcg gttgcctttt tatacacatc aatcaaacat
     3541 tcgtgcattg agcgagatag cagcgatgag tttttcaaaa gagatgaatc aaagcgattt
     3601 ttcgccttat gtattagagg cagtcttact ttggccatat aatgaaattc gtagccaagg
     3661 gatcttagaa gaaataaaag aaatagaaaa aagatgtacg gagtatgatt tagattacaa
     3721 ataaagagac tgtgacaaaa gtcgtgtcac agtccttttt ttccgaataa acggtgttct
     3781 cctgtcaact cctcggtcac aagtcaaaat attcaacaaa catgagaagc tgttttttga
     3841 aattgttcct tgtgactcgg agatgaacgg caggctcacg acctcttgga aaaaggtgtt
     3901 caaaagcttg catctgcggt aataagaaca gccaagttaa aaacataaga agctattttt
     3961 acttggctgt tcttattact tgatgcaggg cagctttttc acaacctctc aataaacggt
     4021 gtgcaatcag ctgaccctcg gtattagctc aaaaaaaacg gcaaaatatg aggaactatt
     4081 ttgccgttta tttctctaat actcaggtca aaacgctgat tccaaaacct cttatttgat
     4141 gggtctgtcc tgaatatctt caaaataatc ggggtaatta tgaatcacgt attcttgctc
     4201 ctcgataatc atatgtaaat gcatcgtgca aagaaatccg acttcatcaa agttatgatc
     4261 aatcattgat tgaagcattg tctgatgttc atctaatact cgaccccagt ctaattctga
     4321 aatcgtcaaa cgtaaccatc tgaaacggtt caagtgcgta ctataacttt ctagccaatc
     4381 ccaaatttct cgttttccag caatttcata acaagttcgg tgaaaatcag tatccaaatc
     4441 aaaaaaagct tttttatttt gagcgatcac tgcctgatcg gtttgttcta gtattttttc
     4501 tagtttagct tgatcttgct tggtcatttt cgcactgcat tcttgcagga tcggtttttc
     4561 caacttttcc cgcgtataac gggctaatga agcagatcgg atatcgattt ttgaaacata
     4621 agtgccggac tgtggaatgg tttttacaag ctcttgattg cgcagttgga tcagtgcttc
     4681 tcgaatcggt gtacgcccga tagctagttg ttcttctaac actttatcgg agattttact
     4741 tccgggtgct aactcggaat aaatgatttg tttacgtata gattcataag cttgctgttg
     4801 caaattattg ataaccattt aaccttcctt tctatgcaga tataatctat tataacataa
     4861 atcgcttaca aatcgtgtgg gcataaaaac acagtctgca aaaaaagccc tgataggcat
     4921 gcctattaga gcctcgaaaa gaaaaatatt gaatcaatct ggtcgtacct cttggatcgg
     4981 tttgacacga ccgccgattc ttttagcaaa agcttcggat tcttttttgt ctttgaaaat
     5041 gagcagattg tctaagtttt tttcccgagt gcgacagcca gttttatcga tgtaaccgtg
     5101 cattacttta acgacataca tggggatcac ctgctttttt cttttcatta tacgaactac
     5161 tcgacacaat acaagaaaaa tgcttagacg gtctttatta agaatttaca tcatccaaag
     5221 aagcgtacgc ttggaataac tgttctaact ccggattgac tttgctcagc ttcactaatc
     5281 gttgcgaatc agaaagtaaa aaacagtgtt tactactgcc agtagttttt aattttttgt
     5341 cttcagttcc cacaatttcg tagctaaaat ccaaccgtgt tcccgtatac ttttggatag
     5401 ttgggtggat gctcactttt tctccgtaat ggatcatttc tttatagctg caatttactt
     5461 ccagaacggg gatgatgatc ccagattctt cgattttttt atagggaaac cctagatgta
     5521 ccaataaggc tacccgagcc tcttcaaacc agcggatata attgctgtga tgaataatcc
     5581 ccatttgatc tgtttcataa taaaaaggtt ctcgcacata acctgtaaat ttctccatga
     5641 ctcatgcctc gttttccatt gattttttac aaaaataatt catccacttc ggaagtgctt
     5701 cgctgatttg tgtcggcaaa acaatatatt gttcttttgc cagctggtct ccaatgaaac
     5761 tatgaagata aacagctgct gcaatcgttt ggtcattttg aggaaactgg gctaaaaatc
     5821 ctgtgatcat cccagctaaa gtgtcaccca tgccgcctgt tgccatagca gcagaaccta
     5881 aaggattggt gtagaagtgc tggtgaccat aaatttttgt ctcatgactt ttcaaaacga
     5941 gcacagcacc gatttcctgt tgcttctttt tgttttcttt atcccgttgt tcttgtatcg
     6001 gtagagaact caaccgttgc cactccattt gatgaggtgt aagtacagtt tgttcgggat
     6061 agggtagcaa aagatgatta gcaacaagta aacttaaagc agagccatca atcaccagcc
     6121 attgttcttt tgattggttt tctagtactt gtttgaaacg ttccaaactc ttagaagaag
     6181 tacccaatcc gggaccaatc aagagtacat ctgcttgctt tattacggaa agaaactggt
     6241 cagtatccgt ccaatctaca atcattgctt ctggcagatg actgtgaaga gcactccgat
     6301 ttttttcatc agttactaca gtcgtgagac ccgctccact gtgtacggca gctaaagcac
     6361 tcataatgat tgctccgcca tattgttcat tgccgccaat caaaacgata cgtccaaatg
     6421 ttcctttatg tgaaatcttt ggacgaggtg tgatgactct tttcactagt tgttcgttta
     6481 tcagcatcga aaaacctcct tttactcatt atagcgtata ttttttttag agaaaaacag
     6541 ataagaaaag gaattgtcag attttttgaa ctattttttt ctgtaatttg tggtatcatg
     6601 aacacgaaga cttaaataag tggaggatta actttatgac aattgattgg caaaaagaag
     6661 tagaagcacg taaagaagac ttgcttgaag acttgaaaaa tcttttacgt gtcaacagcg
     6721 agcgcgatga ttcaaaagta acaccagatg caccatttgg acctggacct cgcgacgcat
     6781 tgaaacatat gttagcttat ggcgaacgtg atggatttaa agtaaaaaat gtcgataact
     6841 atgcagggca tattgatctt ggcgaaggag acgaaacgct agggatattt ggacacatgg
     6901 atgtcgttcc tgcaggagat ggctgggaca cagatcctta tgaaccagta atcaaggatg
     6961 gaaagatctt tgcacgcgga tcaagcgatg acaaaggtcc aagtatggca gcttactatg
     7021 cgatgaagat cattaaagaa cttgacttga aactatcaaa aaaagttcgc ttcgtagtcg
     7081 gcagcgatga agaaagcggc tggggcgata tggcttatta tttcgaacat gaagaagaac
     7141 cagattttgg tttctcacca gatgctgaat tcccaatcat caatggtgaa aaaggaaatg
     7201 tttctttagc tttacgcttc aaaggcgaca atgcaggaga ttatgtcttg aaatcttttg
     7261 tttccggact tcgcgaaaat atggtgccag gaacagcaac tgcagcactt caagtaccaa
     7321 gtgcagatgc agctatcgcg atggaagaag cattttacca attcatcgaa gcaaatccag
     7381 taagcggaac gattgaagcg gacaatacgt atgtaaaaat cgaattagtt ggtaaaggcg
     7441 cacatggtgc aagcccacaa tctggtatca atgcaggttc tttcttggca ttgttcttag
     7501 ataactatga attcctagga tctgctaaac aatttatcca tgtagcagct gcttatgtcc
     7561 atgaagattt ttatggagaa aaactaggag tcgcttatga agatgaaaaa atgggcaaat
     7621 tgacaatgaa tgctggacta tttgcttttg aagaaaatgg tacagaggaa gcgaacttca
     7681 tcaatatgaa tttccgtttt ccaaaaggtg taacagtaga cggattgcaa agtgacatcg
     7741 aacaaacagt ggggcaagaa ggcgcaacag ttactcgagg tgctcgcgta atggaaccac
     7801 attacgtacc aatggatgat ccattggtgg ctacattatt gcaagtctat gaagaccaca
     7861 ctggagaaaa aggctacgaa caaatcatcg gtggcggaac ttacggacgt ttgttgaaac
     7921 gtggcgttgc ttatggagcg atgttcccag gatatactga tacaatgcat caagcaaacg
     7981 aatttatgag tcttgatgac ttgttcagag caacagcaat ctatgcagat gccatctatc
     8041 gtttggcgaa ataaaaatac taaaaa
//
LOCUS       DASHHB010000092         7784 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.096, whole genome shotgun sequence.
ACCESSION   DASHHB010000092 DASHHB010000000
VERSION     DASHHB010000092.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7784)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7784)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7784
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.096"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(192..1484)
                     /locus_tag="U2K71_002614"
     CDS             complement(192..1484)
                     /locus_tag="U2K71_002614"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002359213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HEM7316627.1"
                     /translation="MSSVVVVGTQWGDEGKGKITDFLSENAEVIARYQGGDNAGHTIK
                     FNGVTYKLHLIPSGIFYKDKISVIGNGVVVNPKSLVKELAYLHEKGVETSNLRISDRA
                     HVILPYHIKLDQLQEDAKGENKIGTTIKGIGPAYMDKAARVGIRIADLLDKEIFEERL
                     RLNLDDKNRQFVKMFDSEPLDFDDIFEEYYEYGQQIKQYVTDTSVILNDALDAGKRVL
                     FEGAQGVMLDIDQGTYPFVTSSNPVAGGVTIGSGVGPSKINKVVGVCKAYTSRVGDGP
                     FPTELFDETGEQIREVGREYGTTTGRPRRVGWFDSVVMRHSKRVSGITNLSLNSIDVL
                     SGLETVKICTAYELDGELIYHYPASLKELNRCKPIYEELPGWSEDITGCKTLAELPEN
                     ARNYVRRISELVGVRISTFSVGPDRTQTNILESVWAQI"
     gene            complement(2264..3631)
                     /gene="dnaB"
                     /locus_tag="U2K71_002615"
     CDS             complement(2264..3631)
                     /gene="dnaB"
                     /locus_tag="U2K71_002615"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016623169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HEM7316628.1"
                     /translation="MNEVWQDRIPPQNIEAEQAVLGSVFLDAETIIDAMEYIEPKDFY
                     RRGHQIIFETMIELNDRNEAIDVVTVKDRLEQANLLEDAGGLSYLSDLALAVPTAANI
                     VYYAKIVEEKSLLRTLIQTATGIVTKGFEQGEEVQTILDDAERSILEVSEKRNRSGFL
                     SIAEVLNSTIENIDRLAQNNEEITGLPTGYQGLDKMTAGFQPEELIILAARPAVGKTA
                     FALNIAQNVGTKTDRSVAIFSLEMGAESLVNRMLCAEGSIEASHLRTGQLSEEEWSNL
                     IVAMGSLSRANIFIDDTPGIKISEIRAKCRKLAQEKGNLGLILIDYLQLIEGNGKENR
                     QQEVSDISRQLKKLAKELKVPVIALSQLSRGVEQRQDKRPVLSDIRESGSIEQDADIV
                     AFLYRDDYYEREGGDDDDGNEPKNDNVIEVIIEKNRSGARGTVELLFIKEYNKFSSLS
                     PREEF"
     gene            complement(3905..4357)
                     /gene="rplI"
                     /locus_tag="U2K71_002616"
     CDS             complement(3905..4357)
                     /gene="rplI"
                     /locus_tag="U2K71_002616"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002371936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HEM7316629.1"
                     /translation="MKVIFLQDVKGKGKKGDIKEVPAGYAQNYLIKNNLAKEATKSSI
                     SQLAGQKKAQEKHEAEILEEAKKLKEFLEKEDTVVELKAKAGEDSRLFGSIPSKQIAE
                     GLNKQYKIKLDKRKIELENPIRSLGYTNVPVKLHHEVTATIKVHVVQE"
     gene            complement(4363..6336)
                     /locus_tag="U2K71_002617"
     CDS             complement(4363..6336)
                     /locus_tag="U2K71_002617"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HEM7316630.1"
                     /translation="MENKNNHLSPKPLFLILFILIEVLVVLILPFRFVSASVILLMNL
                     WGVRYWLKMKRWLILNKREKIQQASTVAETNLAYVSEVMPVGVIAYQPSSQKIEWLNP
                     LALKIKDQSGLTEEELLDTFFTAKEKKKNQVYLADKTFQFDMDTVKGVVYFNDITQNQ
                     LLKLHQTNSQPVIGILSIDNYADAIDKLDDKEISYLNSFVTTLVSDWMNEYHVFFKRI
                     NAERFFFVAQMIDLKKMMDKKFDLLDRFRAEAEKQSSPITLSMGIAYGSRTLDEIGGE
                     AQNNLDVALVRGGDQVVVKEVKEGAKPVYYGGKSAKNVKRTRVRSRAMSTALRRIFDE
                     TGDVFIMGHKFPDMDALGSAFGVSCLARFNQKKAYIVINENEIIPDVERCLEEIHKHP
                     ELEMQLISPERAIELKKEEDLLVMVDYHKPSLSISQGLYEQFDKIVIIDHHRRGEEFP
                     SKPLLTYIESSASSASELVSELIQYQSSKTNRLDRFEATLLLAGMVVDTNSFNVRTTA
                     RTFDVASYLRSCGADASLVQYLLSSDLATYLEISNLIAGSDYVTSDIVVAAGTDEKEY
                     NSVIAAKTADTLLSMININAAFVITKRTDGLIGISARSTGTINVQIIMESLGGGGHFT
                     NAATQLENTTVEETKQKLFAAIKENMNQIYDKE"
     gene            complement(6479..6715)
                     /locus_tag="U2K71_002618"
     CDS             complement(6479..6715)
                     /locus_tag="U2K71_002618"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HEM7316631.1"
                     /translation="MAQQRRGGRKRRKVDYIAANHIEYIDYKDIELLKRFISERGKIL
                     PRRVTGTGAKNQRKITVAIKRARIMGLLPFVSED"
     gene            complement(6741..7253)
                     /gene="ssb"
                     /locus_tag="U2K71_002619"
     CDS             complement(6741..7253)
                     /gene="ssb"
                     /locus_tag="U2K71_002619"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010750861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HEM7316632.1"
                     /translation="MINNVVLVGRLTKDPDLRYTASGTAVATFTLAVNRNFTNQSGNR
                     EADFINCVIWRKSAETLANYARKGTLLGVTGRIQTRSYDNQQGQRVYVTEVVADNFQL
                     LESRSASENRQQSGGFQSSGQSAGGFGGNNNSNQTSQSSNGMPDFDRDTSDPFGSSST
                     IDISDDDLPF"
     gene            complement(7303..7602)
                     /locus_tag="U2K71_002620"
     CDS             complement(7303..7602)
                     /locus_tag="U2K71_002620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005226363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HEM7316633.1"
                     /translation="MENTKYEIMYIIRPNIDEEAKTALIERFDAILKDNGAEVIESKD
                     WEKRRFAYEMNGYREGIYHIVKVSSPSSANAVNEFDRLAKINDDIIRHMIVKEEA"
BASE COUNT         2150 a         1650 c         1400 g         2584 t
ORIGIN      
        1 ttcggaaaaa gggactgtga cacggttttt gtcacagtcc cttattctga ataaacagtg
       61 ttcaagagac agcctatcgg caataaggct aaacgtctct ttcactacct cttcaagtta
      121 ttattatcgt atagataaac acttcgactc gctctggtaa gaacgtcctg attcagtcgt
      181 cgtttttatc cttagatttg cgcccagacg ctttctaaga tgtttgtttg tgttctatca
      241 ggtccgacag aaaatgttga gatacgtaca ccaacaagct ctgagatacg acgtacatag
      301 ttacgtgcgt tttctggcag ttctgcaagt gttttgcatc ctgtaatgtc ttctgaccag
      361 cctggcaatt cttcatagat tggtttgcag cggttcaact ctttcaagct tgctggatag
      421 tggtagatta gttcgccatc taattcataa gccgtacaaa tttttactgt ttccaatcca
      481 cttaatacat cgatcgagtt taaagatagg ttggtaatac ctgaaactcg tttagaatgg
      541 cgcataacta cagaatcaaa ccagcctaca cgacgcggac gaccagttgt cgtaccgtat
      601 tctcgtccta cttcgcggat ttgttctcct gtttcatcaa ataattcagt tggaaatggt
      661 ccatcaccga cacgtgacgt ataggctttg cagacgccga caactttatt gatttttgaa
      721 ggccctacac cgcttccgat tgttacccca ccggctactg ggttagaaga agtaacaaaa
      781 ggataagttc cctggtcgat atccagcatt acaccttgtg cgccttcaaa caacacgcgt
      841 ttgcctgcat ccaaagcatc attcaagata acggaagtat ctgtgacgta ttgtttgatt
      901 tgttgtccgt attcataata ttcttcaaaa atatcgtcaa aatcaagtgg ttctgaatca
      961 aacattttta caaattgacg gtttttgtca tctagattca aacgaagacg ttcttcaaaa
     1021 atttctttat ctaataagtc tgcaatacgg atacctacac gagctgcttt atccatatag
     1081 gcaggtccga ttcctttaat cgttgtacca atcttatttt cgcccttagc atcttcttgt
     1141 aattggtcca atttgatatg ataaggcaag attacgtgcg cgcggtcaga aatacgcaaa
     1201 ttacttgttt ctacaccttt ttcatgtaaa taagctaatt ctttaaccaa tgatttagga
     1261 ttgactacaa cgccgtttcc gatcacactg atcttgtctt tataaaaaat cccagaagga
     1321 atcaaatgca gtttataagt cacaccgtta aacttaattg tgtgccctgc attgtcgcca
     1381 ccttggtacc gtgcaatcac ttcagcattt tcactgagaa agtctgtgat ttttcctttc
     1441 ccttcatcgc cccattgcgt acctactact actacagatg acatgtgaac acctcgttct
     1501 atattattta atcctgaacc attgtatcaa taatagatac atatttcaaa taaaatcgaa
     1561 tgatttttat ttttatttta taaaaacgct taaaaaacga actttatctt tttagaatgg
     1621 ttattaaaaa agaggttgta acagaagtgg tcagcttcaa gaaataagaa ggaatgtacg
     1681 aaaattgctt ctcaaatttt tgtgaaattt cgacttattt ttcgaagaag ctacttttgg
     1741 aacaccgtga atcagaggtt gtgaaaaagc tgtcctgcat caagtaataa gaacagccaa
     1801 acaaaaatag cttctcatat ttttcgtttg attgggcgta ttaccgcaga tgcaagcttt
     1861 tgaacaccgt ttaagaaaga ggttgtgagc ctgccgttta actctgagtc acaaggagca
     1921 atttcaaaaa actgcttctc atgtttgatg atattgtgac ttgtgaccga ggagttggca
     1981 ggagaacacc gtttaaggaa aagaggtcgt aagatcagcg ttttgacctg agtattagag
     2041 aaacaaacgt caaaatagtt cctcatattt tgacgtttgc tgagctaata ccaagggtca
     2101 gctgattgaa caccgtttat tcggaaaaag ggactgtgac aagacttttg tcacaatccc
     2161 tttggagatc tgtttaaatt aagtactcta gtcgttcctg cggttactcc ttgcatcttc
     2221 ttacgagagc tctcgggatt gctcaaggct cacaacctct tctttaaaat tcttctcttg
     2281 gcgaaagcga cgaaaactta ttgtattctt tgataaacag tagttcaact gttcctcgtg
     2341 caccgcttcg gttcttctcg atgatgactt cgatgacgtt gtcatttttt ggctcgtttc
     2401 catcatcgtc atctccgcct tctctttcat aataatcatc tcgatacaag aaggctacga
     2461 tgtccgcatc ttgttcaatt gatccggatt cacgaatatc acttagcact ggtcgtttgt
     2521 cttgtcgctg ttctactcca cgagataact gtgaaagagc aatgacaggt actttcagct
     2581 ctttcgccag ttttttcagt tggcgagaga tatctgatac ttcttgttga cggttctctt
     2641 taccgttacc ttcaatcaac tgcaaataat cgatcaatat caagcctaga ttgccttttt
     2701 cttgcgctag tttccgacat ttcgcacgga tttcagagat tttgatccct ggtgtatcat
     2761 cgataaaaat atttgctcga gaaagactcc ccatcgcaac gatcagattc gaccattctt
     2821 cttccgaaag ctgacctgta cgtaaatggc tggcctcaat cgaaccttct gcacaaagca
     2881 tccgatttac aagagattct gcccccattt ccaaactaaa tatcgcaaca gaacgatctg
     2941 tttttgttcc tacattttga gcaatattca atgcaaacgc tgttttacct accgctggac
     3001 gtgcagcaag aatgattagt tcttcgggct ggaaacctgc tgtcatttta tccagacctt
     3061 gatagccagt tggtagacct gtgatttctt cattgttttg agctagtcga tcgatatttt
     3121 caatcgtgga gttcagtact tcagcaatag ataaaaagcc gcttcgattt cttttttccg
     3181 aaacctccaa gatactgcgt tcggcatcat ctaaaattgt ttgaacttct tctccttgct
     3241 cgaatccttt cgtaacgatt cctgttgctg tctggatcaa tgtacgcagc aatgattttt
     3301 cttcaacgat tttcgcatag tagacgatat ttgctgctgt cggaactgct aatgccaagt
     3361 ctgagagata acttaaacca ccggcatctt ctaacaaatt ggcttgttcc agccggtctt
     3421 tgacagtaac cacgtcaatc gcttcattac gatcatttaa ctcgatcatt gtctcaaaaa
     3481 taatttgatg gccacgacga taaaagtctt ttggctctat gtactccatg gcatcaatga
     3541 tcgtttctgc gtcaagaaaa acagagccta aaaccgcttg ttcagcttca atattttgcg
     3601 gtgggattcg atcctgccac acttcattca tacgtatcgc tccttgtctt caattgcttc
     3661 attttacaag aaaatgagag aaaagtcatc attcgtaccg aaagtgtaat aaaaaaatag
     3721 ttctctttta ttatactcgt tttctttatg ctattttgct gttcccaaaa aagaaaccga
     3781 aatcaatctg aaatcgattt gcagcttttg ctttcattgc tgcagctgtt ttctcagata
     3841 aatttcggtt tgtctttttt ccttctggtt atacttacca gctgaacatc gctatcaaga
     3901 tttgttattc ttgtacgaca tgaactttga tcgtagccgt cacctcatga tgaagcttaa
     3961 caggtacgtt tgtgtagcct agtgaacgga tcggattttc taattcgatt tttcgtttat
     4021 ctaatttgat tttgtattgt ttgtttaacc cttcagcaat ttgttttgaa gggatcgaac
     4081 caaacaaacg gctgtcttcc cctgctttcg ccttcaattc gactacagta tcttcttttt
     4141 ccaagaattc ttttaacttt ttagcttctt ccaagatttc agcttcgtgt ttttcttgtg
     4201 ctttcttttg tcctgctaac tgactgatac tgcttttcgt tgcttctttt gccaagttat
     4261 ttttgattag ataattttgt gcataacctg ctggaacttc tttgatgtcc ccttttttac
     4321 cttttccttt tacatcttgt aaaaaaatta ctttcatttg gattactcct tatcatatat
     4381 ttgattcata ttttctttga ttgcagcaaa tagcttctgt tttgtttcct caactgtcgt
     4441 attttccagt tgagtagctg cgtttgtgaa gtgtcctccg ccacctaagg attccatgat
     4501 gatttggacg ttgatcgtgc cggtacttct ggcactgatc ccaatcaagc cgtctgtccg
     4561 ctttgtaatg acgaaagctg cattgatatt gatcatcgaa agcaatgtat ctgctgtttt
     4621 tgctgctata acactattat actctttttc gtctgtccct gccgccacaa caatatcaga
     4681 agtcacatag tcgcttccag caatcaaatt gctgatctcc aagtatgtcg ctaaatccga
     4741 gctcaataaa tattgtacta aggatgcgtc tgctccacaa gaccttaaat agctggcaac
     4801 gtcaaaagtt cgtgcagttg tacggacatt gaaactattt gtatctacaa ccatccctgc
     4861 aagcaataat gtggcctcaa aacgatctaa gcgatttgtt ttgctgcttt ggtattggat
     4921 cagctcagaa actagctctg aagctgaaga agctgaagat tcgatataag tcagcagagg
     4981 tttacttgga aactcttccc cacgacgatg gtgatcaatg atcacaatct tatcaaattg
     5041 ctcatatagg ccctgagaga tcgatagaga aggtttatgg taatccacca tcacgaggag
     5101 gtcctcttct tttttcagct ctatggcacg ttctggagag attagttgca tttctaattc
     5161 aggatgctta tggatttctt caagacaccg ttctacatct ggaatgatct cgttctcgtt
     5221 gataacgatg taagcttttt tctggttgaa gcgtgccaga caactgacac caaaagcaga
     5281 gcctaaagca tccatatccg gaaacttgtg ccccatgata aacacatcgc ctgtttcatc
     5341 aaaaatccga cgcaaagcgg tactcatcgc tcgagaacga acacgcgtgc gtttgacatt
     5401 cttagcagat ttccccccat aataaacagg ttttgctcct tcttttacct ctttcacgac
     5461 aacttggtcg cctccacgga ctaaagcaac atctaaatta ttctgagctt ctccaccgat
     5521 ttcatcgagg gttcttgagc cataagcaat ccccatacta agggtgatag gcgagctctg
     5581 tttttcagct tctgctcgaa agcgatccag caaatcaaat tttttatcca tcatcttttt
     5641 cagatcgatc atctgtgcta cgaagaaaaa ccgttctgcg ttgattcttt tgaaaaagac
     5701 atggtattca ttcatccagt cagaaactag tgtagtgacg aaagagttca aataagaaat
     5761 ctctttgtca tccaatttat caatggcatc tgcatagtta tcaatagata aaataccaat
     5821 caccggctga ctgttcgtct gatggagttt taacaactgg ttttgggtaa tatcattaaa
     5881 ataaacaaca ccctttactg tatccatatc aaactgaaat gttttatctg ctagatagac
     5941 ttggtttttc tttttttctt ttgcagtaaa aaaagtatct agcaattctt cctctgttaa
     6001 tccactttga tctttgattt tcaatgctaa tggattgagc cattcgattt tttggcttga
     6061 tggctgataa gcaatcactc ctacaggcat gacttctgag acataagcta gattggtttc
     6121 agctacagtc gaagcctgct ggattttttc acgtttattt aaaataagcc aacgtttcat
     6181 tttcaaccaa tagcgaacac cccataaatt catcaataga atgaccgaag cgctaacaaa
     6241 acgaaatggt aaaattaaga ctaccaatac ttcgattaat ataaacagga taaggaagag
     6301 aggtttcggg gataaatgat tgtttttatt ttccataatt ttcctcccat atatgactaa
     6361 tttgatttta ccataacttt cgtcattcga gcgactaaac gaatagaggt tatttttaca
     6421 gaaaaaaagg aattgaatag ctgatcaaaa ggatcagtca ttcaattcct aggatttatt
     6481 agtcttcgct aacgaaaggt agcaagccca tgatacgagc gcgtttgatt gcaacagtaa
     6541 ttttacgttg gtttttagcg cctgtaccag ttacacgacg tggcaagatt ttaccgcgtt
     6601 ctgaaataaa tctttttagt aattcgatat ctttataatc gatatattca atatggttag
     6661 cagcaatgta gtctacttta cgacgtttac gtccgcctct tctttgttgt gccattctat
     6721 ttccctccta tcaaattcgt ttagaatggt aaatcatcgt ctgaaatgtc gatcgttgaa
     6781 cttgagccga atgggtcaga agtatcacgg tcgaaatcag gcataccgtt ggatgattga
     6841 gatgtttgat tagagttatt gtttccgcca aatccgccag ctgattgacc agaactttgg
     6901 aatccgccgc tttgttggcg attttcagat gcagaacgac tttccagcaa ttggaagttg
     6961 tcagctacta cttctgtcac gtatacacgt tgtccttgct gattatcata agaacgagtt
     7021 tggatacgtc cagtaacacc taaaagtgtt cctttacgag catagtttgc taaagtttct
     7081 gctgatttgc gccaaatcac acagttgata aaatcagcct cgcgattgcc gctttggttt
     7141 gtaaaattac gattcacagc caaagtaaat gttgcgactg ccgtaccact tgctgtgtaa
     7201 cgcaaatctg gatctttcgt cagacgtcca actaatacaa cattattaat caaaatagtc
     7261 atctcctctc atcgttttct attgtttcac atgaaacaac aattaagctt cttctttaac
     7321 aatcatgtga cgaatgatat cgtcgttgat tttagcaaga cggtcaaatt cattgacagc
     7381 atttgctgat gatggagaag atactttaac gatgtgatag ataccttcac ggtaaccgtt
     7441 catttcataa gcgaaacggc gtttttccca atcttttgat tcgataactt ctgctccgtt
     7501 atctttcaag attgcatcga aacgttcgat taaagcagtt tttgcttctt catcaatgtt
     7561 tggacgaata atgtacatga tttcatattt cgtgttttcc attgatattc acctccctgt
     7621 ggactttcgg ctcttaggtt cctaagagca aggagagtta tctaattaat tagactactc
     7681 acaagctctg attatacact ctattttggt caaaagcaat acagaatgaa cagtttcgaa
     7741 tattttaaga gaaattcaac taatcaaaaa agccagcagg aata
//
LOCUS       DASHHB010000093         7598 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.097, whole genome shotgun sequence.
ACCESSION   DASHHB010000093 DASHHB010000000
VERSION     DASHHB010000093.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7598)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7598)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7598
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.097"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(941..1354)
                     /locus_tag="U2K71_002621"
     CDS             complement(941..1354)
                     /locus_tag="U2K71_002621"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002291922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HEM7316634.1"
                     /translation="MANVPSIRDMFPDFNDVFSPAFNDFLGVSSYPKVDLVENEKEYK
                     LTADMPGCDKEDTTVEYSDNTLTISANHESHTEDKEDGNYVRKERHSVSYKRSFYLPN
                     VDEEKITGTFKNGVLKLVLPKTAYQPKETKKIELN"
     gene            complement(1810..2295)
                     /locus_tag="U2K71_002622"
     CDS             complement(1810..2295)
                     /locus_tag="U2K71_002622"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316635.1"
                     /translation="MHKGIYAGLLGTVILFSACGYKTKNSVKESSAAISTTEVGSTKS
                     TSTTSITNTSTGNVDSQHSQSSIQSFSISSTSEMQESTVPSLPLENSDKALLFLIAHR
                     SELQSDDIVISFYQKIDRDYLFTASSKQIRSQGGSGSVGFYRVSPEGSITMTDANGTP
                     F"
     gene            complement(2434..2649)
                     /locus_tag="U2K71_002623"
     CDS             complement(2434..2649)
                     /locus_tag="U2K71_002623"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF378 domain-containing protein"
                     /protein_id="HEM7316636.1"
                     /translation="MKTLDAIALTLLIVGGLNWLLVGLFELDLVAMIAGGSTTIFAKI
                     IYIVVGICAIYCLKFFPMITRKVDERY"
     gene            2873..3673
                     /locus_tag="U2K71_002624"
     CDS             2873..3673
                     /locus_tag="U2K71_002624"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM7316637.1"
                     /translation="MSDWLDIAGKVVIVTGGSSGIGRSIVENLLKQNAQVANFDVTEC
                     RIQHENLLSLKVDVSSKTDIEEGIYKVMKHFKTIDGLVNNAGINIPSLLIDRNHPKSK
                     YELSEQVFDKMIAVNQKSVYLMSQAVGRILVQKGSGVIVNLSSESGLEGSEGQSCYAA
                     TKAAMNSFTRSWAKELGKRNVRVVGVAPGILEETGLRTQEYEEALSYTRGISVEQLRN
                     GYSRTSTIPLGRSGKLQEVADLVCYYLSERSSYITGVTTNISGGKTRG"
     gene            3692..5560
                     /locus_tag="U2K71_002625"
     CDS             3692..5560
                     /locus_tag="U2K71_002625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEM7316638.1"
                     /translation="MTLVNRWYQLLKLLVTHKKVSIQEVQKEIKTSNQTIKKDIGELN
                     EEIAGIAKIIQKNKHFELEIYDFDFFDEIMQGKLKTASDFNSASKRIAYLLNRLIETE
                     DFLRMDDLSEEVGVSRGTVVKDLKTLKEMINDFDVKITGTPNKGLRIIGDELELRLLL
                     FYFVSPYFSETKTEQFLEEKQAIKHFQKKTNASKQEITLLTKVIGISLNRISSNYLLK
                     KPINRYSGCFEYIQEFNELINQLEEIYELTLSQYEKDFISFPLNIINTSITMIRKANS
                     DLRLYFDPMMMRIRRLDLIDLDESFLYQEMKQHLSLLINRVIFRYRLTDLFYGEIEKK
                     YPFSYQIASECIQALEAILQHKISKVEISYLTLYLELAFRYQKEHFSSKKVAIVHTTG
                     KGTALMIQRQIKRILGRDIQITHVSEEQYEKVDLNKYFAVFTTIPLKNVNEETPVIHL
                     TNLFDDSWLRAQWQKAKKKRLAGFHSTVFFYQIWKEKKTYQEYLNKLVAPLIRGYYVD
                     EEFEKRILKREYQQSTFVNKEFAFPHTTNQLSQHIVLSVGLITDQGLAETGGNGVIFL
                     FAIPEEMTAEVENELFELCDILFELIKMGIDEFIELAPTQESFLKLIKSKGERLHE"
     gene            5553..6044
                     /locus_tag="U2K71_002626"
     CDS             5553..6044
                     /locus_tag="U2K71_002626"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390786.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR family transcriptional regulator"
                     /protein_id="HEM7316639.1"
                     /translation="MNDMYLFIVFAASAYVLQAVISWKQLKHFNQTYSELRRKGKVAI
                     GKRPGKIRSGTIVMLAIDDHDQVLEVRKMQGVTSLAKFHSLPVYAGEDIHYIDRYHPS
                     VRAEDRLTQAALENAREVYLRIEAGNEKSEQPSLPFIAMLNNFRIWKSTIKEKRSDKR
                     WIS"
     gene            6032..6586
                     /gene="srlA"
                     /locus_tag="U2K71_002627"
     CDS             6032..6586
                     /gene="srlA"
                     /locus_tag="U2K71_002627"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327877.1"
                     /note="catalyzes the phosphorylation of incoming sugar
                     substrates along with their translocation across the cell
                     membrane; the IIC domain forms the PTS system
                     translocation channel and contains the specific
                     substrate-binding site; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIC"
                     /protein_id="HEM7316640.1"
                     /translation="MDIITKFAEGFMRLFQTGAETFISWMTGIVPVVLMLMVAMNTLI
                     AFLGEDRVTKVAKLSAKNPLTRYLVLPFLSAFMLGNPMSFTMARFLPEYYKPSYYAAQ
                     AQFCHTSNGVFPHINPGELFVWMGIAQGIETLGLNSMELAIRYLLVGLLMNFIGGWIT
                     DFTTGFVCRQQGIVLSKKVELSVD"
     gene            6604..>7598
                     /locus_tag="U2K71_002628"
     CDS             6604..>7598
                     /locus_tag="U2K71_002628"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucitol/sorbitol transporter subunit IIB"
                     /protein_id="HEM7316641.1"
                     /translation="MYGDIKIVRGNGGYGGPLIIRPTKDKHKFIYVTGGGERPMIVDK
                     IEELTGMESINGFKTSIPDSEIALAIVDCGGTLRCGIYPKKGIPTINIVSTGKSGPLA
                     QYITEDIYVSAVGLEQIQPLMETDDNKETVNTASPDKKYKYDTSKKITEQKAEQQNWI
                     AKIGMGAGKVVATFNQSAREAVDTMIRTVIPFMAFVSLLIGVIQGSGIGNVFANIMAP
                     LAGNAVGLTIIGFICSLPFLSPLLGPGAVISQVIGTLIGVEIGKGNIPPQLALPALFA
                     INTQNACDFIPVGLGLEEAKAETVEVGVPSVLYSRFLNGIPRVIIAWVASFGLYS"
BASE COUNT         2706 a         1213 c         1444 g         2235 t
ORIGIN      
        1 tgttactatg aataacataa taagaaagta aagtggattg atttgagatc tttttatact
       61 tcaaaaaaga taacacccaa ctttcaaaaa tatgttttgg aagttgggac aaacgagtta
      121 atacatttta atacattatt ttttattcaa aaaaaatagc acaatggcaa agatcgtaag
      181 agagatcata agccccaatg aatgactaaa aagaaaagca gaatcacatg ccatatcatc
      241 ccatatttct tcaagaaaat aaataagcgg gaaaaaggga aggaatagta gaacatcgat
      301 ccagcaccat ttttttcttc ttttgaaata acataaaaca ataaacatga acgtgcaggg
      361 aagtaaaaac aaagctttac tcataactaa caaaatacca gctcctttta tttttttctc
      421 ccccacattc aaagagtaac aatggtatgt acttaataat agaaaaatgg atcaacttgt
      481 atgaataaca gctgaaaaga tctgcaccaa ataaaaaact tccagccaaa actgactgga
      541 agtttatttt tttctgaaat attctaacac aaatatgaaa aagatggccg aagctaataa
      601 gccgatttcg tagcaaaaca atgagatacc agagtcgtaa taataaaatc ctctaatctt
      661 atggataaaa tagatcatcg gacaagcaga taagagtaag tatttgttag gccttttgaa
      721 taagtaaaag aaagcactgg tcattatgat aaatggtatc caaaacaaaa gtttacttaa
      781 gacaatcaaa tgatcaactc ccatattttt taccaagaga gatcataaca tctttatcgt
      841 aaaaacaaaa tatttttttg ctcattttga aaatttaaac atactcgttc ttatccaaat
      901 atataaaaat cgcccctcat tcatcgagag gcgataagtt ctaatttaac tcaatttttt
      961 ttgtttcttt tggctgataa gctgttttag gtaataccaa tttcagcaca ccgtttttaa
     1021 aggttccagt gattttttct tcatcaacat taggaagata gaatgaacgt ttgtaagaga
     1081 ccgaatgtct ttctttccgt acatagttgc catcttcttt atcttctgta tgggactcat
     1141 gattggcgga gatcgtcaaa gtattatctg aatattcaac tgtcgtgtct tctttgtcac
     1201 agccaggcat atcagcagtt agtttgtatt ctttttcatt ttcgactaaa tctacttttg
     1261 gataacttga aacacctaaa aaatcattga atgctggact aaacacgtca ttgaaatcag
     1321 ggaacatgtc gcgaatgcta ggtacatttg ccatttttga tcatcctttc aataagtatt
     1381 ttttatttat ccctatttac gtaataactc tattttgaaa aaaatgcaag tataatggtt
     1441 agctttgtga atatttcatc taatacgata caaaggctat ctttctaatt tgaaaaaggg
     1501 atcattaaaa taataagaag aaaatatgtc aaaaggtaaa aaagtaaatt cttctatcca
     1561 aatatagaaa aattgaatct agaaaaccgc ccgacaataa atttcattaa ctcagttgac
     1621 cgaaaatgaa gtagatacca aaaatttatc aagagagcaa gtagagaagt gagtatattc
     1681 ataagagaaa tagctgtaac gaattattag gttaatgcaa atgcttattt atactccgta
     1741 taactttctc agaaaaaatg ataccaataa tgacttgcta gtactgtaaa tccgtataat
     1801 atcatctaat caaaatggtg ttccatttgc atctgtcatc gtaatcgatc cttcgggtga
     1861 cactcgataa aaaccaacgc ttccagatcc tccttgggaa cggatttgtt tgctggaagc
     1921 tgtgaacaaa taatctcgat caattttttg ataaaagcta ataacaatat cgtcactttg
     1981 taattcggaa cggtgggcaa taagaaatag aagtgctttg tcagagtttt ctaatggaag
     2041 agaaggtaca gtgctttctt gcatttcaga tgttgaagaa atgctaaacg actgaatact
     2101 tgattggcta tgttgcgaat ctacattacc agtagaagta ttagtaatgg aagtagtaga
     2161 agtactttta gttgaaccaa cctcagttgt tgaaatggct gctgaagatt ctttaacaga
     2221 attctttgtt ttatatccac aggcagaaaa aagtattaca gttcccaaca agcctgcgta
     2281 aatcccttta tgcatgatag ctcctcctaa aatagattat ctttattcag tatatcactt
     2341 aaacaaagaa aagcagatgg tttaaaatga aaaaaccttc ccaataaagg gaaggttcat
     2401 gaagataata acttttttaa taattatagc tttctaatat ctttcatcaa cttttctagt
     2461 aatcattggg aaaaatttca agcagtagat cgcacaaata cctacgacga tataaatgat
     2521 cttcgcaaaa atagttgttg aaccaccagc aatcatagct actaaatcaa gttcgaacaa
     2581 gcctaccaac agccagttca accctcccac aatcaataaa gttaatgcaa tagcatctaa
     2641 agttttcatt agaattctcc tttcttaaat gataaaacaa ttatatatca aaaagagaaa
     2701 cattgcttat ataacgctca ggaattttag caatctatgc aaaaagatga aaattttttt
     2761 tgctcttcca ttagtacaca ttttttagct gaagctgtaa atttcatgtc ttaatttttt
     2821 ttagtgagct tttatactgg atttgtaaga aaaaagaagg agaggatcac atatgtcgga
     2881 ttggttggat attgcaggaa aagtagtaat tgtaactgga ggttcctcag gaattggaag
     2941 aagtatcgtc gaaaatttgt tgaagcagaa tgcacaagta gcgaattttg atgtaactga
     3001 atgccgtata cagcatgaaa acctactatc tttaaaagtc gatgtgtctt ctaaaacgga
     3061 catagaagaa gggatatata aagtaatgaa acatttcaag acgatcgatg gtctagtcaa
     3121 taatgcagga ataaatattc cttctcttct aatagataga aatcatccaa aaagcaagta
     3181 tgaattatca gaacaagttt ttgataagat gatcgctgtc aatcaaaaaa gtgtatattt
     3241 gatgagccaa gcagttggaa gaatcttggt gcaaaaggga tcaggagtga tcgtgaatct
     3301 ttcttctgaa tcaggattgg aaggatcaga aggacagagc tgttatgctg ctacaaaagc
     3361 agcgatgaac agttttactc gttcttgggc aaaagagtta ggaaagagga atgttcgtgt
     3421 ggtaggtgtt gcaccaggga tactggaaga aaccggtttg agaacacaag aatatgaaga
     3481 agctctttct tatacgagag gtataagcgt cgaacagctg agaaacggtt acagtcgaac
     3541 aagtacgatc cctctaggaa ggagtggtaa gctgcaagaa gtggcggatc tagtctgcta
     3601 ttatctttca gaaagatcaa gttatatcac tggagtcacg actaatattt caggaggtaa
     3661 aacaagagga taaaggatag gggggacgca cgtgacgcta gtaaacagat ggtaccaatt
     3721 attgaaatta ctagtcactc acaagaaagt ttcaatccag gaagtgcaaa aagaaatcaa
     3781 aaccagtaat caaaccataa aaaaagatat tggggagttg aatgaagaaa ttgccggtat
     3841 tgcaaaaatc atccaaaaaa acaaacattt tgaacttgaa atttatgatt ttgatttttt
     3901 tgatgagatc atgcaaggaa aattgaaaac agccagtgat ttcaattcgg catccaaaag
     3961 aatcgcttat ttactaaata ggctgatcga aacagaggat ttcttacgta tggatgattt
     4021 gtctgaagaa gtcggtgtca gcagaggaac agtggtcaag gatctaaaga cattaaaaga
     4081 gatgatcaat gattttgatg taaagattac aggcacacca aataaaggac taagaataat
     4141 aggagatgag ctagaattac gactactttt attttatttt gtttctccct acttttcaga
     4201 gacgaaaaca gaacagtttc tagaagaaaa acaagcaatc aaacattttc agaagaagac
     4261 gaatgctagt aaacaagaaa tcaccctttt aacgaaagtt atcggtattt ctttgaatag
     4321 aatctctagt aactatctgt tgaagaagcc catcaatcgt tacagtgggt gttttgaata
     4381 tatccaagaa ttcaatgaac tgatcaatca gttggaagaa atctatgaac taactttgag
     4441 ccaatatgaa aaagatttta tcagttttcc tttgaacatc atcaacacaa gtataacgat
     4501 gataaggaaa gctaactctg atttgcgctt atatttcgac cccatgatga tgcgtattcg
     4561 acgattagat ctcattgatc tggatgagtc cttcttatat caagagatga aacagcacct
     4621 ctctttattg ataaatcggg taatttttcg ttatcgattg actgatttat tttatggtga
     4681 gatagaaaaa aagtatcctt tttcttacca aatcgctagc gaatgcatac aagcattaga
     4741 agctatcttg caacataaga tctctaaagt agagatcagc tacctaacgt tgtatttgga
     4801 gttagcattt cgatatcaaa aagagcattt ttcctctaaa aaagtagcca ttgttcatac
     4861 gacaggaaaa ggaacagcat taatgatcca aagacaaatc aaaaggatat tggggaggga
     4921 tatacaaata acacatgttt cagaagaaca atatgaaaaa gtagatctaa acaaatattt
     4981 cgctgttttt acaacgattc cgttaaaaaa tgtaaatgaa gagacaccgg tgatccattt
     5041 gactaatctt tttgatgact cttggttaag ggcacaatgg caaaaagcaa aaaaaaagcg
     5101 attagccggg tttcattcta ctgttttttt ctatcaaata tggaaagaaa aaaagacgta
     5161 tcaagaatat ttaaacaaat tagtcgctcc tttgatcaga ggctattatg tggatgaaga
     5221 atttgaaaaa aggatcttga aaagagaata tcagcaaagc acatttgtta acaaagaatt
     5281 tgcttttcct cacacaacca accagctatc tcaacacatt gttttatcag tagggctaat
     5341 aactgatcaa ggtttagcag aaacaggagg aaatggagtt atttttttat ttgccatacc
     5401 tgaagaaatg acggctgaag ttgagaatga attatttgag ctatgcgata tcctatttga
     5461 actcataaaa atgggaatag atgagtttat tgaattagca ccgacacaag aatcattttt
     5521 aaaactaatc aaaagtaaag gagagagact tcatgaatga tatgtatcta ttcatagttt
     5581 tcgcagcttc agcttatgtg ctgcaagcag ttatcagttg gaagcaacta aagcatttca
     5641 atcaaacata tagcgaactg cgaagaaaag gaaaagtagc gattggtaaa cgacctggga
     5701 aaatccgttc aggcacgatt gttatgctag ctatagacga tcatgatcaa gttttagaag
     5761 tcagaaaaat gcaaggggtt acatctttgg caaaatttca ttcattacct gtctatgcgg
     5821 gtgaagatat ccattacatc gatcgttacc atccctcagt tcgagcggaa gatcgtttga
     5881 cacaagcagc tttagaaaat gccagagaag tatacttacg aatagaggca ggaaatgaaa
     5941 aaagtgaaca accttcattg ccttttattg ctatgctcaa taattttcgg atatggaaaa
     6001 gtacgatcaa agaaaaaagg agtgacaaac gatggatatc ataacaaaat ttgctgaggg
     6061 atttatgagg ctgttccaaa caggtgcaga aacattcata agctggatga caggaatcgt
     6121 accagttgtc ttgatgctga tggttgcaat gaatacgctg attgcttttt taggtgaaga
     6181 ccgagtaacg aaagtagcca aattatcagc gaaaaatccg cttactcgtt accttgtgtt
     6241 accgttctta tctgctttta tgttgggaaa tcctatgtca tttacaatgg ctcgtttcct
     6301 tccagaatac tacaaaccaa gctattatgc tgctcaagct caattttgtc atacgagcaa
     6361 tggtgtattc ccccatatta atcctggaga attgtttgta tggatgggga ttgctcaagg
     6421 gatcgaaacc ttagggctaa attcgatgga gctggctatc cgatacttgc ttgtgggact
     6481 gctgatgaac tttattggtg gttggataac tgattttact actggatttg tatgcagaca
     6541 gcaaggaatc gttcttagca aaaaagtaga gctttcagtt gattagagaa aggagagttt
     6601 taaatgtatg gtgatattaa gatcgtaaga ggaaacggcg ggtatggagg tcctttgatt
     6661 attaggccga caaaagataa acacaagttc atctatgtca caggtggagg tgagaggcca
     6721 atgatcgtag ataaaataga agaactgaca ggtatggagt ctatcaatgg gttcaaaaca
     6781 tcaatacctg atagtgaaat cgcgcttgca atcgttgact gtggagggac tcttagatgt
     6841 ggaatttatc ctaaaaaagg gataccaacc atcaatatcg tttccacagg aaaaagtggc
     6901 ccattagcac aatatatcac tgaagatatt tatgtttcag cagtagggtt agaacaaata
     6961 cagccactaa tggaaactga tgataacaaa gaaacagtaa atactgcatc tccagataaa
     7021 aagtacaaat atgatacttc taaaaagata accgagcaaa aagcagagca gcaaaactgg
     7081 atagcaaaaa ttggaatggg cgcgggaaaa gtagttgcta cattcaatca atcagcaaga
     7141 gaagcagtag atacgatgat ccggacagtg atccctttta tggctttcgt ttcccttttg
     7201 attggtgtga tacaaggttc tgggatcggt aatgtatttg ctaacattat ggcaccttta
     7261 gcaggaaatg cagttggatt aacaatcatt ggctttattt gttcactgcc atttttatct
     7321 cctttgcttg gtccaggggc agttattagc caagtgattg gaacattgat cggtgtagag
     7381 attgggaaag gaaatattcc tccccaatta gcattaccgg cactatttgc tatcaatact
     7441 caaaatgcat gtgattttat tccagtcggt ctaggtttgg aagaagcaaa agctgagaca
     7501 gtagaagtag gtgtaccatc agttctttat tcaagatttc ttaacggcat cccacgagtt
     7561 atcattgctt gggtagcaag ttttggatta tatagctg
//
LOCUS       DASHHB010000094         7537 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.098, whole genome shotgun sequence.
ACCESSION   DASHHB010000094 DASHHB010000000
VERSION     DASHHB010000094.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7537)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7537)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7537
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.098"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            107..1248
                     /locus_tag="U2K71_002629"
     CDS             join(107..335,335..1248)
                     /locus_tag="U2K71_002629"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611172.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEM7316642.1"
                     /translation="MAKAKKKFDEDFKKMILDLNQSGQSVEELAAQYGIATQTIYRWK
                     KLHTKNVATGMTEAEILAMKKEMARMQEENTIPKKGFNHIRSKVKMSDVFEFIQSNVH
                     DHDVKQMCTVLEAPKSSYYDWKKKKPSKRQSENKRLNRLISGIYFENKGIYGAPKIHK
                     ILVGRGETLSLKKVQILMRKLGLRSVTLKKYKPGKQAKVKSEGRKNLLNQDFTTTSIN
                     QKWVTDITYIHTLKDGWTYLSTIQDLHTKKVIGWKFGKQMTKELVIETLDNALLNQTP
                     SENLIIHSDLGSQYTSEAYEAKLNELNIRHSFSRKGCPYDNAGIESFHASLKKEEVYP
                     SKAYENFEAAYLALFQYIEGFYNRKRIHSSINYLTPNQMEELALQA"
     gene            1483..1935
                     /locus_tag="U2K71_002630"
     CDS             1483..1935
                     /locus_tag="U2K71_002630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000751236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316643.1"
                     /translation="MKKTILSIFSVFTILIGGYFLLNLDSVNSNAQIKDQRVAEHNNK
                     KMSLTKNIYSMSDEELYKEFGDLSGEQLEESIPGISDSDKIVVLSAGGQLMGDGKSEL
                     VSYKQNHRQFIDVNTDSNAISIKDLKELLNSKRNELKQIKETNSVELE"
     gene            1949..2638
                     /locus_tag="U2K71_002631"
     CDS             1949..2638
                     /locus_tag="U2K71_002631"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000718009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316644.1"
                     /translation="MKKIFGVSVGLLMMLTFGNAVYAAEKEVISDPEQYASRYFADFE
                     EEDKVLALPEGGFLKGEAKIVSMDNPDIIQGSYNSETDPASVSVTEAKKIVAEKFKND
                     IETTPIMPRGAFPPDVGTSGHLLVLAGGGSYKSQPFSGAGWRFGGMGVKPADGTGNFL
                     RWKSYIDDGRVGNVNQANATKSGSIQGMALYAREGFKYFAPNGPGWTMNIGIYYTYNP
                     VAGTYYVVQNI"
     gene            2763..5114
                     /locus_tag="U2K71_002632"
     CDS             2763..5114
                     /locus_tag="U2K71_002632"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000344083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1906 domain-containing protein"
                     /protein_id="HEM7316645.1"
                     /translation="MDEMVLSTQKWLNKKYSNVTGFDKVPENGRTGWPTIYGLIEGLQ
                     VELGITNLVANFGPTTEKMYDNQVTPKWGKNLPKNIVFLIQGAFWCKGINPGGFDGVY
                     TPYLDTAVKELQTDAGFNGSAVTGVLDAKWAKALFDMSAFVLVPGGDSKIRKMQQWLN
                     VNYLEYTGIMPCDGIYQRNSNQALIFALQAELGYSPSEATGSYGNGTTSKTYPVSEGN
                     SGNYVRIIQYGLYVNGYFEDGTFDGIFTKYMGLEVLAFRKFMVLPEYTEIADVVVIKG
                     LLSSAGDIRRSADGADTSTQLTRSQIQTLVDNDIKIVGRYLTGTVGIGKDERNKYLTT
                     EELNNIFSKNLSVFPIYQDGGAALAYFTYDRGLSDGKKAIDAAKNLNIPLTTVIYFAV
                     DLDMLGEEILAYAGEYFKGVSAVMSYSGYQTGVYGTRNVCSQIINNGYASFAFVSDMS
                     TGYSGNLGFPMPRQWSFDQFVEFTIGSGNGAVGIDLIATSGRDSGFNELSNDTNNDNY
                     IAKYNQKVINQMVRAYQYLSQAGLDNPWNPHLTFYRYVNYSGLSWDIISSPVTEHDRK
                     IYDEYRNKLTNSEGLYNYFIDPNTGSIIGLPHLIVTLQSQMFLADTINDSVSDFAGWL
                     GDLMTCWGEVKKLGIQMEQGVFALVGTAAGGTFSLEDLYQDVDAVNVYTLAKRNSDST
                     ALSALKTYYCDKGAIDRFNQFVKNRFGSSDKIASVTNSLLENDETVDILGVGIISFFI
                     DKIVNNSLGTDFTYLDFIGDEGKVANGFSEKIIQFQENRSKLN"
     gene            5194..5589
                     /locus_tag="U2K71_002633"
     CDS             5194..5589
                     /locus_tag="U2K71_002633"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316646.1"
                     /translation="MMIMEEIEWNNLTQNNKNNSGCGIIFMFVIVLGIVVLLITVKIV
                     DYFANTPEEQIQRKLPEFERVPPYDPRDNSFQVKKLKESPNEMWFEVTVESSDFKEKK
                     KEYNYIVKAKVSKRFIFNPKLEIYSVVIE"
     gene            5914..6531
                     /locus_tag="U2K71_002634"
     CDS             5914..6531
                     /locus_tag="U2K71_002634"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001224538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7316647.1"
                     /translation="MRKFFYIRVSSYDQSIERQLISAKELGIPEEYIFVEKASGKDFK
                     RPEYQLMKRMFRKGDLLYLQSLDRLGRNKQMILEEWRELTKEKQIDISVLDMPLLDTA
                     SYKQLDGLETLISDLILQLLSYMAEDERKRINSRQREGVEAAKQKGVKFGRKKIALPP
                     EFPAIYSDWKAGKITAVYAMDQLGMKRNTFYRRVKEYEHTLHHFS"
     gene            6572..6871
                     /locus_tag="U2K71_002635"
     CDS             6572..6871
                     /locus_tag="U2K71_002635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000366408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BRCT domain-containing protein"
                     /protein_id="HEM7316648.1"
                     /translation="MDLTNQRVVFTGTLQQMTRTQAIGLVHSLNGHCQSSVTSKTNFL
                     VCGQYSKSLFDDSLYTKKEQTARDLIALGHEITILSEVEFYALISQQLKEWQRYL"
     gene            6905..7072
                     /locus_tag="U2K71_002636"
     CDS             6905..7072
                     /locus_tag="U2K71_002636"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000824191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
                     /protein_id="HEM7316649.1"
                     /translation="MKRKSYDRWGIEITSFNRNIAFHVECDCGELAKSVYQKNYFYCP
                     KCHRRYGVHGT"
     gene            complement(7117..>7537)
                     /locus_tag="U2K71_002637"
     CDS             complement(7117..>7537)
                     /locus_tag="U2K71_002637"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7316650.1"
                     /translation="SLDRLGRDYEDIKNTVAFMKQKKVALKILDAQFLDFNTGNELLD
                     TAMFDMFLSLLSYIAQNEREKIRERQRQGVLLAQKAGRYKGRPIEYSINSTDPQKRLV
                     FKTVVDMLKQGIPVAEIAKENGISRPTIYKIKKANDL"
BASE COUNT         2594 a         1138 c         1502 g         2303 t
ORIGIN      
        1 attcctcctg ctatcttttt ctattattct atcttatttg atagtagatt taaaactttg
       61 caacagaacc gtagaatcta actactaaga aaaggacgtg tccacgatgg caaaagccaa
      121 gaaaaaattt gatgaagact ttaagaagat gatcttagat ttaaaccagt ctggccaatc
      181 ggtcgaagaa ctggcagcgc aatatggtat cgctacacaa acgatttatc gttggaagaa
      241 gcttcataca aagaatgtag caaccggcat gactgaggca gaaatcctag cgatgaaaaa
      301 ggaaatggcc cgcatgcagg aggaaaacac tatcctaaaa aaggctttaa ccatattcgc
      361 tcaaaagtaa aaatgagtga tgtcttcgaa tttattcaat caaatgttca tgatcatgac
      421 gtaaaacaaa tgtgtaccgt tttggaagca ccgaaatcca gctactatga ctggaagaaa
      481 aagaaacctt ccaagcgcca atcagaaaac aaacgcttaa accgcttgat ttcaggaatc
      541 tactttgaaa acaagggtat ctatggtgcg cctaaaattc ataagattct tgttggccgt
      601 ggcgagacct tgtccttaaa gaaagtacaa atactgatga gaaaattagg cttgcgttca
      661 gtcaccctca agaagtacaa gcctggcaaa caagcgaaag ttaaaagtga aggacgtaaa
      721 aacctgttaa accaagattt taccactacg tcgatcaatc aaaaatgggt gacagatatt
      781 acctatattc ataccctaaa agatggctgg acctacctct ccactattca agacctccac
      841 acaaagaaag tcatcggttg gaagtttggc aagcagatga cgaaagaatt ggttatcgaa
      901 acccttgaca atgcgctctt aaaccaaaca ccctccgaga acttgatcat ccactcagat
      961 ttgggttctc agtacactag cgaagcctat gaggcaaagc tgaatgaatt gaacattcgg
     1021 cactccttca gtcgtaaggg ctgtccctac gataatgccg gtatcgaatc gtttcacgcc
     1081 tcactcaaaa aagaagaagt ctatccatca aaagcgtatg aaaactttga agctgcttat
     1141 ttagcgttat ttcaatacat tgaaggattt tacaatcgca agcgaatcca tagtagtatc
     1201 aactatttaa caccaaatca gatggaggag ttggcattgc aagcttagtt cctagctacc
     1261 acttacattt ttttggcgag atttccacag ggtttcaact tcatcgcttc gatcagcggt
     1321 caaacggttt tggtttgacc gctggtcgaa acgatgagat actcacccgc tggaatctcg
     1381 aaaaaattct cacaaatcgt gtccgaggta ttgactcaaa tccaccaatt atcttatcgt
     1441 agatatatat tataaattta ataagaaagc gaggtgagaa tcgtgaaaaa aacgatacta
     1501 agtatttttt cagtatttac aatattaatt gggggctatt ttcttttgaa tttagattct
     1561 gttaattcaa acgctcaaat aaaagatcaa agagtagctg aacataataa taaaaaaatg
     1621 agtttgacca aaaatatcta ttctatgtca gatgaagaat tgtataaaga atttggtgat
     1681 ttatccgggg aacagttaga agaaagtatt ccaggaatta gtgactcaga taaaattgtt
     1741 gtcttgtctg ctggtgggca attaatggga gatggcaaat ccgaattagt tagttataaa
     1801 caaaatcaca gacagtttat tgatgttaat actgattcca acgctatttc gattaaggat
     1861 ctaaaagagc ttttaaatag taaaagaaat gaattaaagc agataaaaga aactaattca
     1921 gtggaattag agtaaaggaa gattcattat gaaaaagatt tttggtgttt ctgttgggct
     1981 acttatgatg ttaacatttg gaaatgcagt atatgctgct gagaaagaag ttatttcaga
     2041 tccagagcag tacgcctcac gatactttgc tgattttgaa gaagaagata aagtacttgc
     2101 attgccagaa ggtggttttt taaaagggga agctaaaatt gtatctatgg ataatccaga
     2161 tataattcaa ggttcatata attcagaaac tgatcctgct tcagtttcag tgacagaagc
     2221 aaaaaaaatt gttgctgaaa aattcaaaaa tgatattgaa actactccaa ttatgcctcg
     2281 aggagcattt ccacccgatg ttggtactag cggacacctt cttgtactag caggaggagg
     2341 aagctataaa tcacagccat ttagtggtgc gggatggcgt tttggaggta tgggtgtaaa
     2401 accagctgat ggtacaggca actttttacg ttggaaatcc tacattgacg atggaagagt
     2461 aggaaatgta aatcaagcca acgcaactaa atctggttct attcaaggaa tggcattgta
     2521 tgctcgtgaa ggattcaaat actttgcacc aaacgggcct ggttggacaa tgaatatagg
     2581 tatttattac acttataatc ctgttgcagg tacgtattat gttgttcaaa atatttaata
     2641 aaacagcaca aagagatttc tttgtgctgt tttattattt ttagttgttg acaaacacta
     2701 aaatataaaa tataatcaac acataaacga acgtatgttc ggcaagaaag caggggattt
     2761 ttatggatga gatggtttta agtactcaga aatggctaaa caagaaatac agtaatgtta
     2821 cagggtttga taaggttcca gaaaatgggc gaacagggtg gcctacaatc tatggactta
     2881 ttgaaggact acaagtagaa cttggtatta ctaatctagt agctaatttt ggacctacca
     2941 ctgaaaaaat gtacgataat caggttactc ctaaatgggg caaaaatcta cctaaaaata
     3001 ttgtatttct tattcaaggt gccttttggt gtaaggggat taatccaggt ggcttcgatg
     3061 gggtatatac tccatattta gatactgctg taaaagagtt gcagactgat gcagggttta
     3121 atggaagcgc tgtaactgga gttttggatg cgaaatgggc caaagcgtta tttgatatga
     3181 gtgcttttgt tcttgttccc ggcggagata gtaaaattag aaaaatgcag caatggttaa
     3241 atgtaaatta tttagaatac acgggaataa tgccttgtga tggtatttat caaagaaatt
     3301 caaatcaagc attaattttt gcattacaag ctgaactagg atattctccc agtgaagcaa
     3361 cagggtctta tggtaatggg acaactagta aaacttatcc tgtgtctgaa ggtaactcgg
     3421 gaaactatgt aagaataatt caatatggat tatatgttaa tggttatttt gaagacggta
     3481 cattcgatgg tattttcact aaatatatgg gtttagaagt actggcattc cgaaaattta
     3541 tggtattgcc tgaatatact gaaatagcag atgttgttgt aataaaagga ctattgtcta
     3601 gcgctggaga tattagaaga tctgcagatg gcgcagatac ttctacgcaa ttaactagat
     3661 ctcaaatcca aacattggtt gataatgata taaaaatagt tggtagatat cttactggta
     3721 ctgttggaat tggaaaagat gaacgaaata agtatctaac tacagaggaa ttgaataata
     3781 ttttttctaa aaatttatct gtgtttccta tctatcaaga tggaggtgca gcattagctt
     3841 attttactta tgatcgtggc ttatcagatg gtaagaaggc tatagatgca gcaaaaaatc
     3901 taaatatacc attaacaaca gttatttatt ttgctgttga tctagacatg ttaggggaag
     3961 aaattcttgc atatgctgga gaatatttta agggggtatc tgctgtaatg tcatatagcg
     4021 ggtatcaaac gggtgtttac ggaacaagaa atgtttgttc tcaaataatc aataacgggt
     4081 atgcttcgtt tgcatttgtt tcagatatgt cgactggtta tagtgggaat ttaggttttc
     4141 ctatgcctag acaatggtct tttgatcaat ttgttgaatt taccatagga agtggcaacg
     4201 gagctgttgg tatcgattta atcgctacat cgggtagaga tagcggattt aatgagttat
     4261 caaatgatac taataatgat aattacattg ccaaatacaa tcagaaggtt ataaatcaga
     4321 tggtacgtgc gtatcaatat ctatcacaag ctggtcttga taatccttgg aaccctcatt
     4381 tgacatttta tagatatgta aattattctg gtttgtcatg ggacatcatt tcttctcctg
     4441 taacagagca tgatagaaaa atttatgatg aatataggaa taaattaact aatagcgagg
     4501 gcctgtataa ttattttata gatcctaata ctggttcaat aataggtttg cctcacttaa
     4561 ttgtgacgtt acaatcacag atgtttctag ccgacacaat caatgatagt gtttctgact
     4621 ttgcaggttg gttaggagat ttaatgactt gctggggtga agttaagaaa ttaggaatac
     4681 aaatggagca aggtgtattt gcattagtag gtacggctgc aggtggaaca tttagtctag
     4741 aagatttata ccaagatgtt gacgcggtaa atgtttatac cttagcaaaa agaaattctg
     4801 attcaacagc tttatcggct ttaaaaacgt actattgtga taagggtgct attgatagat
     4861 ttaatcaatt tgttaagaac agatttggtt cttcagataa aatcgcttca gtaacaaata
     4921 gtttattaga aaatgacgaa acagttgata tcttaggtgt agggataatt tcatttttca
     4981 ttgataaaat agttaataat tcacttggga cagattttac ttatcttgat tttattggag
     5041 atgaggggaa agttgcaaat ggattttctg aaaaaattat tcaatttcaa gaaaatagaa
     5101 gtaaacttaa ttaagaatcg tttactattt aaacagtgtc cttttccttt ttaaatatat
     5161 acgataaaat aagtatatga attgtaaaag gacatgatga ttatggaaga aatagaatgg
     5221 aataatctga ctcaaaataa taaaaataat tctggatgtg gaattatctt tatgtttgtt
     5281 atagttttgg gaatagtagt acttttaata actgtcaaga tagtagatta ctttgcgaat
     5341 actcctgaag aacaaataca gagaaaactt cctgagtttg aaagagtacc accttatgat
     5401 ccaagagata attcatttca agtaaaaaaa ttaaaagagt ctccaaatga aatgtggttt
     5461 gaagttacag ttgaatcaag cgacttcaaa gaaaaaaaga aagaatacaa ttatattgta
     5521 aaggcaaaag tatcaaaaag atttattttt aaccctaaat tagaaatata tagtgttgta
     5581 atagaataat agtgttaaaa aaattgtaaa agttaaagaa gtcaaattct gatgtgaaaa
     5641 ataaaatata ccatggaaga cataaaagaa aaactggata actagtttat ttaatggcag
     5701 accgttaagg cctgccattt ttatgtattt tagcggaata aatataatca tgcgggagtg
     5761 taaacttact gtttcaaaaa gtatactttt tgacatgcct tgattttctt tgtttatgta
     5821 tggcataata accacataat acaatttgaa acgtttctga tttaatctga ttgttttgaa
     5881 acaattttag tgaaaaaagg gtagataaaa acgatgagaa aattttttta tatccgggtc
     5941 agtagttatg atcaatcgat cgaacgacaa ctgatttccg cgaaggaatt aggaatccct
     6001 gaagaatata tatttgtaga aaaagccagc ggcaaagatt ttaaacgccc tgaatatcaa
     6061 ttaatgaaac ggatgtttcg aaaaggagat ttactctatc tacaatcatt agatagacta
     6121 ggcagaaata aacaaatgat tttagaagaa tggcgagaat taactaagga aaagcagatt
     6181 gatatttctg tgttagacat gcctctgtta gatactgcta gttacaagca actagatggg
     6241 ttagagacgc ttatctctga cttaattctg caattactca gctatatggc cgaagacgag
     6301 cggaaacgaa ttaatagccg gcaaagagaa ggcgtagaag ctgcgaaaca aaagggtgta
     6361 aaattcggca gaaagaaaat tgctttacct cctgaatttc ctgcgatcta ttctgactgg
     6421 aaagcaggaa agataactgc agtctatgct atggatcaat tgggcatgaa acgaaatacc
     6481 ttttatcgac gggtgaaaga atatgaacat accttgcacc acttttcttg atagatataa
     6541 cagctattag acgggaaggg agagtttgaa aatggactta acgaatcaaa gggtcgtttt
     6601 tactggaacg ctgcagcaaa tgacacgaac ccaagccatc ggtttggttc actctttaaa
     6661 cggccattgt caatcaagtg ttacttcaaa aacaaatttc ctggtttgtg gtcagtattc
     6721 taagtcgttg tttgatgatt ctctctatac aaaaaaggaa caaaccgcgc gggacttgat
     6781 tgcgttaggt catgagatta ccattttatc agaagtcgaa ttttatgcgt taattagtca
     6841 acagttgaaa gaatggcaac ggtatttata atcgttagta aaatttacaa ggaggaaaat
     6901 ttttatgaag cgaaaatctt atgatcgttg gggcattgaa ataactagtt ttaatcgaaa
     6961 tattgctttt cacgtggagt gtgactgtgg ggaattagca aaaagtgttt atcaaaaaaa
     7021 ttacttttac tgtcctaaat gccatcgccg atacggggta catggaacat agaaacgaaa
     7081 tagtgcggta gaaaagcgaa tgaatgtagt atatttctac aaatcattcg ctttctttat
     7141 tttataaata gttggtcgag agatcccgtt ttcttttgca atctctgcaa ctggaatgcc
     7201 ttgctttaac atgtccacca cggttttaaa aactaagcgt ttttgcggat ctgttgagtt
     7261 aatagaatac tcgattggtc gtcccttata tcggccggct ttttgagcca ataatactcc
     7321 ttgccgctga cgttcacgaa ttttctcacg ctcgttttgt gcaatataac ttagcagtga
     7381 taaaaacata tcgaacatag cggtatccag gagttcattt ccagtattaa aatctaaaaa
     7441 ctgtgcatct aatattttta acgctacttt tttctgtttc ataaaagcaa ctgtattttt
     7501 tatatcttca taatctcttc ctaaacgatc tagtgat
//
LOCUS       DASHHB010000095         7390 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.099, whole genome shotgun sequence.
ACCESSION   DASHHB010000095 DASHHB010000000
VERSION     DASHHB010000095.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 7390)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7390)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7390
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.099"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(204..1652)
                     /gene="cls"
                     /locus_tag="U2K71_002638"
     CDS             complement(204..1652)
                     /gene="cls"
                     /locus_tag="U2K71_002638"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cardiolipin synthase"
                     /protein_id="HEM7316651.1"
                     /translation="MKIIVDNFFTILLIMNILLSFIIVFRERKETAQTWAWLLVLMFI
                     PVVGFILYIFLGRGISKEKIFDLKIQGKIGKNLEIEEDKQALMRGLYPHPPTGQVDVK
                     QLIYMLTVFESTLYTTKNEITLFTDGREKFDALIEDIKQAEDHIHFQYYIYRSDALGE
                     EVRDALTEAARRGVKVRVLLDAWGSTQVSSSFFQNLKKAGGEVAFFFPLFVPYINPRI
                     NYRNHRKIVVIDGEIGYTGGFNVGNEYLGLVKKFGYWRDNHLRIYGEAVYTLQNRFLM
                     DWNSQHTKEVGYSPKFFPSIHTTGDIAVQIVTSGPDTEHEQIKMTYLKMISLAKREIL
                     IQTPYYIPDGSIHEALKLALLSGVKVHIQIPNKPDHLLVYWATYSFAAELIEYGARIE
                     TYENGFIHAKTMIIDGGIVSVGSANIDVRSFRLDFEVNTLVYDERMASRVRKAFFEDS
                     KISTHLTKEMYENRGIIIKMKEGLARLISPLL"
     gene            complement(1804..2697)
                     /locus_tag="U2K71_002639"
     CDS             complement(1804..2697)
                     /locus_tag="U2K71_002639"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7316652.1"
                     /translation="MDTMIRTFFRHLLESIKSLKRNGWMTVASVSAVTITLTLVGVFM
                     AVIMNATKLAQDIEGNVDVSVFVDIGTKDEEMKKLEKELNDLDHVKKVEFSSKEQELK
                     KIQNEMGDSWKLFEGDNNPLYDVYVVSATDPSYTKQVAEEAADLKNVSRSDYGGASSD
                     RIFQIAGAVRTWGLVAAALLLFVAMFLISNTIRITILSRQREIQIMRLVGAKNGYIRW
                     PFFLEGGWIGLIGAILPILIITFGYAWFFQLINPSLLRSHYSLIHPQNIVWKINLLMV
                     GIGVIIGSLGSIISMRRFLKF"
     gene            complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="U2K71_002640"
     CDS             complement(2681..3367)
                     /gene="ftsE"
                     /locus_tag="U2K71_002640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005237293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell division ATP-binding protein FtsE"
                     /protein_id="HEM7316653.1"
                     /translation="MIEMKDVMKKYSNGTTAIRNLSIVIEQGEFVYVVGPSGAGKSTF
                     IKLMYREEKASKGTLNVAGHDLIAIKNREVPYLRREIGVVFQDYKLLPRKTVYENVAY
                     AMQVIGRKPRDIKKRVMEVLDLVGLKHKVRVFPSELSGGEQQRVSIARAIVNTPKVLI
                     ADEPTGNLDPENSWEIMKLLDRINAQGTTVVMATHNSTIVNTIRHRVIAIENGRIIRD
                     QAEGEYGYDD"
     gene            complement(3472..4573)
                     /gene="prfB"
                     /locus_tag="U2K71_002641"
     CDS             complement(join(3472..4500,4502..4573))
                     /gene="prfB"
                     /locus_tag="U2K71_002641"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_139124933.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
                     /protein_id="HEM7316654.1"
                     /translation="MEISEIRNLLTEMQEKVTSFRRSLDLDQLEEDIAEAEAKMAEPG
                     FWDDSEAAQKVISENNANKETYDQFHSLAEELEEIELMVEMLQEEPDAAMQEEAETRI
                     NALDEKMKTYELSMLLDGPYDRNNAIVELHPGAGGTESQDWGSMLLRMYTRWAEQHGY
                     QVETLDYQAGDEAGIKSVTLLIKGHNAFGYLKSEKGVHRLVRISPFDSAKRRHTSFCS
                     VDVMPELDETIDIEINPDDLKIDTYRASGAGGQHINKTESAVRITHLPTGTVVASQAQ
                     RSQLKNREQAMGMLKAKLYQLEVEKKEQEAAALRGEQKEIGWGSQIRSYVFHPYSMVK
                     DHRTNVETGNVQAVMDGDLDLFIDGYLKMHLQ"
     gene            complement(4699..7233)
                     /gene="secA"
                     /locus_tag="U2K71_002642"
     CDS             complement(4699..7233)
                     /gene="secA"
                     /locus_tag="U2K71_002642"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecA"
                     /protein_id="HEM7316655.1"
                     /translation="MANLIRSLIENDKKELKRLDSIAKKVEAYADQMAALTDEQLQAK
                     TAEFKGRYQKGETLDQLLPEAFAVVREAAKRVLGLYPYHVQLMGGIVLHDGNIPEMRT
                     GEGKTLTATMPVYLNALTGEGVHVVTVNEYLATRDSTEMGELYNFLGLTVGLNINSKS
                     SDEKREAYNCDITYSTNNELGFDYLRDNMVVYRHQMVQRPLNYAIVDEVDSILIDEAR
                     TPLIISGQAEKSTALYTRADNFVKRLKEEEDYKIDVQSKTISLTEAGIEKAEETFGLD
                     NLYDIENTALTHHLDQALRANYIMLLDIDYVVQDGKVLIVDQFTGRIMDGRRYSDGLH
                     QAIEAKEGVEIEDETKTMATITFQNYFRMYKKLAGMTGTAKTEEEEFREIYNIQVYQI
                     PTNRPVIRDDRPDLLYPTLQSKFHAVVQDIKDRYRKGQPVLVGTVAVETSELLSDMLN
                     QERIPHEVLNAKNHFKEAEIIMNAGQKGAVTIATNMAGRGTDIKLGLGVRELGGLAVI
                     GTERHESRRIDNQLRGRAGRQGDPGMSQFYLSLEDDLMKRFGSERIKAFLDRMKIGEE
                     DAVIQSKMLTKQVESAQKRVEGNNYDTRKNVLQYDDVMREQREVIYKQRQEVIMEEKS
                     LSKPLMNMVKRTISRVVDAHTQLEKENWNLESIIDFAGSTLVHEDSITLADIEGKTAQ
                     EIKEYLNERAQEVFNTKAAQLNGPEQLLEFEKVVILRVVDSKWTDHIDAMDQLRQSIG
                     LRAYGQNNPLVEYQTEGYKMFEDMVGAIEYEVTRIFMKAEIRQNVQREQVSQGTASHS
                     EDGDAKENSNTSAKKQPIHVNKVGRNDPCPCGSGKKYKNCHGKNAN"
BASE COUNT         1975 a         1639 c         1289 g         2487 t
ORIGIN      
        1 gctatactgg ctttgaaatg tgtctctttt aatagcatat attagcttat ttcttgcttt
       61 ccccaaaaaa caagagatac cccaaagtta atttcgctca tgcgagacgc tttctccatt
      121 tctgacgtac tctcagaaat ggccccgtta aaaaaggaca cgagatctcc atctgttgat
      181 ggttctcatg tcctttttat gttttaaagg agcggcgaaa tcaatcttgc taatccttct
      241 ttcatcttga tgattattcc tcggttttca tacatttctt ttgtgagatg cgtagaaatt
      301 ttcgagtctt caaaaaaagc ttttcttaca cgtgaagcca ttcgttcatc ataaaccagt
      361 gtattgacct caaagtccaa gcgaaatgaa cgaacatcga tatttgctga gccaactgaa
      421 acaatgccac catcgataat catcgtcttt gcatgaataa atccattctc atatgtttcg
      481 atccttgctc catactcgat cagctctgca gcgaaagaat acgttgccca gtaaacgaga
      541 agatgatctg gtttgttagg tatttggata tgaactttta cgcctgaaag aagagccaac
      601 ttcagtgctt catgaatcga tccatccggt atgtaataag gcgtttggat caatatctcc
      661 cgtttggcta acgaaatcat tttcaaataa gtcattttga tttgttcatg ttcggtatct
      721 ggtccgcttg tcacgatttg aacagctata tcaccagtag tgtgaatgga cggaaaaaat
      781 ttgggagaat agcctacttc ttttgtatgc tgagaattcc agtccatcaa aaaacggttt
      841 tgcaaggtat acaccgcttc tccgtaaata cgcaaatgat tgtctcgcca ataaccaaat
      901 ttctttacca atcctagata ttcatttcca acattgaacc cacctgtata tccgatttcc
      961 ccatcaataa ctacgatttt acgatgattg cgataattga tccgaggatt aatatacgga
     1021 acaaatagag gaaagaaaaa tgcgacttct ccgcctgctt ttttcaaatt ctgaaaaaag
     1081 ctggaagaaa cttgtgtgga accccatgca tcaagaagga ctctgacttt tacccctcgt
     1141 cttgccgcct ctgtcaatgc atctcgaact tcttcaccta atgcatcact gcgataaata
     1201 taatactgga agtggatatg gtcttctgcc tgcttgatat cttcaatcag cgcatcgaat
     1261 ttttcacgac catctgtaaa taaagtgatt tcatttttag tcgtatagag cgtactttca
     1321 aataccgtga gcatatagat caattgtttc acatctactt gtccagtagg cggatgagga
     1381 taaagccctc gcatcaatgc ctgtttgtct tcttcaattt ccaggttctt accgattttc
     1441 ccttggatct tcaaatcaaa gattttctct ttggaaatcc ctcttcctaa aaaaatatat
     1501 aaaataaatc ctacaactgg aatgaacatc aaaacgagca gccatgccca tgtttgagca
     1561 gtttcctttc tttctcgaaa aacgatgata aaggaaagca aaatattcat aatcaaaagg
     1621 atcgtaaaaa aattgtcaac gataattttc acggcttcac ctcttcttct accaacttaa
     1681 gaatacccaa gttgtttttt aaagtaaagg gaaaaaagcc gcttccttct attctcctag
     1741 ccaataaaaa aagaaagctg gaacgaaact gtccaacttt ctcattttat ttatattgtg
     1801 gtattagaat ttcaagaaac gtctcataga aatgatcgag ccaagtgaac caatgatcac
     1861 accaatgccg accattaaaa gattgatttt ccaaacgata ttttgtggat gaatcaatga
     1921 ataatgggaa cgtaataatg atggattgat caattggaaa aaccatgcat aaccaaatgt
     1981 gatgatcaaa attggaagaa ttgcaccgat cagaccgatc cagccacctt caaggaaaaa
     2041 cggccagcga atatatccat tttttgcacc gactaaacgc atgatttgaa tttcacgctg
     2101 tctagacaaa atcgtgatcc gaatcgtgtt cgaaatgaga aacatcgcta cgaacagcag
     2161 caatgccgct gcaactaatc cccatgtccg aactgcacca gcgatttgga aaatccgatc
     2221 tgaggaagct ccaccgtaat cagaacgaga aacattcttc aaatctgctg cttcttctgc
     2281 tacttgtttt gtataagaag gatcagtagc acttactaca tacacatcat ataacgggtt
     2341 attgtcacct tcaaacaatt tccaggaatc acccatttcg ttttggattt ttttcaattc
     2401 ctgttctttg cttgaaaatt caactttttt aacatgatct aagtcattca attctttttc
     2461 tagctttttc atctcttcgt cttttgtacc gatatctacg aatacagata catcaacgtt
     2521 tccttctata tcctgagcta atttagttgc attcatgata actgccatga atacaccgac
     2581 taaagtcaac gtgatcgtta ccgcactgac tgaagcaact gtcatccagc cgttacgttt
     2641 caaactcttg atactttcta ataaatggcg gaaaaaggtt ctaatcatcg tatccatatt
     2701 ccccttccgc ttgatctcga ataatgcggc cgttttcgat agcaattact cggtgccgaa
     2761 tcgtatttac gatcgtactg ttatgtgtag ccatcacgac agtagttcct tgggcattga
     2821 tccgatctaa taatttcatg atttcccatg agttttctgg atccagattt cctgtcggtt
     2881 catccgcgat aagtacttta ggcgtattca cgattgcccg agcaatagag acacgttgct
     2941 gttccccacc ggataattcg ctaggaaaga ctcgaacttt gtgtttcaaa ccaacaaggt
     3001 ccagcacttc cattactcgt tttttgatat ctcgaggttt tctgccgata acttgcatag
     3061 cgtacgcaac attttcatat actgtttttc ttggtaataa tttatagtct tggaacacca
     3121 cgccgatttc tctgcgtaga taaggaactt cgcggttttt gattgctatt agatcatgac
     3181 ctgcgacgtt caaagttcct tttgaagctt tttcttctcg atacatcaat ttgatgaatg
     3241 tagatttacc ggctccagac ggaccaacca catagacaaa ctctccttgt tcgatgacaa
     3301 tcgagagatt gcggatagcg gtcgtaccat ttgagtactt cttcattaca tccttcattt
     3361 caatcatggc gttctctcca tatctttttc agtctttagc tattatagca tggcaatgtt
     3421 tcaacatgct tgcaaaatct tacaatttta tttcaattta acaatgggtt cttattgtag
     3481 atgcattttt aaatagccgt caataaataa atcaaggtca ccgtccataa ctgcttggac
     3541 atttcctgtc tctacgtttg tccgatggtc ttttaccata gaatagggat ggaaaacata
     3601 agaacggatc tgtgaacccc agccgatttc tttttgttct cctcgaagtg cagctgcttc
     3661 ttgttctttc ttttccactt ctaattgata aagcttagct tttaacatcc ccatcgcttg
     3721 ctcacggttt ttcaattgtg agcgttgtgc ctgactagct actactgttc ctgtaggcaa
     3781 gtgagtgatc cgtacagcag attctgtttt attgatgtgc tgaccacctg caccacttgc
     3841 tcggtacgta tcgattttca aatcatctgg attgatttct atatcgatcg tttcatcaag
     3901 ctctggcatc acatctaccg aacaaaaaga agtatgacgt cgtttcgctg agtcaaaagg
     3961 cgaaatccgt acaaggcgat gaacaccttt ttctgatttt agataaccaa aagcgttatg
     4021 tcctttaatc agcagggtca cactcttgat ccctgcttca tcacctgctt gataatctag
     4081 tgtttctact tgatagccat gctgctctgc ccaacgtgta tacatacgta aaagcatgct
     4141 gccccagtct tgggactccg ttccgccagc ccctggatgc aattcgacga tcgcattgtt
     4201 tcgatcataa ggtccgtcta acagcataga taattcatac gtcttcattt tttcatctaa
     4261 tgcattgata cgtgtttctg cttcttcttg cattgctgca tcaggttctt cttgcagcat
     4321 ctccaccatc aattcgattt cttctagttc ttcggcaaga gaatggaatt ggtcataggt
     4381 ttctttatta gcgttatttt cgctgatcac tttttgtgcg gcttcgctgt catcccaaaa
     4441 accaggttca gccatttttg cttctgcttc cgcgatgtct tcttccagct gatctaagtc
     4501 aaagagacct cctgaaactt gtgacctttt cctgcatttc tgtcaggaga tttcgaattt
     4561 cggatatttc catgtttttt ccttctttca ctatactttt ttcattatat ctagtctaac
     4621 agaatcagca aataacagaa agaaggtgag acgaattctt aaaaaaagaa tttgtctcac
     4681 caatcctgct gtcagaaatt agtttgcatt ttttccatga cagttcttat attttttccc
     4741 gcttccacaa ggacaaggat catttcttcc gaccttattc acatggattg gctgtttttt
     4801 cgcgctagta ttactatttt ctttggcatc tccgtcttct gagtgagaag ctgttccttg
     4861 agaaacctgt tcacgctgta cgttttgacg gatctcagct ttcataaaga ttcgagtgac
     4921 ttcatactcg atggcgccta ccatatcttc aaacatctta tagccttctg tttgatattc
     4981 gactaatgga ttgttctgtc cataagcacg taagccgatt gactgacgta attgatccat
     5041 tgcatcaata tggtctgtcc atttagagtc cacaacacgc aagatcacga ctttttcaaa
     5101 ttccaacagt tgctctggac catttagttg agccgctttg gtattaaata cttcttgtgc
     5161 tcgttcgttt agatactctt tgatctcctg agctgttttt ccttcgatat ctgcaagagt
     5221 aatgctatct tcatggacaa gagtactgcc ggcaaaatcg atgatacttt ccaaattcca
     5281 attttctttc tctaattgtg tatgcgcatc tactacacga ctgattgtcc gtttgaccat
     5341 gttcattagt ggcttggata agcttttttc ttccatgatt acttcttgtc gctgtttata
     5401 gattacttca cgttgttcac gcatcacatc atcgtattgt aagacgtttt tacgtgtgtc
     5461 atagttattt ccttctacac gtttttgtgc agattctact tgtttcgtca gcatcttgct
     5521 ttggataaca gcatcttcct caccgatctt catccgatct aagaaagctt tgattcgttc
     5581 tgaaccgaat cgtttcatca aatcatcttc caatgacaag tagaactgtg acataccagg
     5641 gtctccttga cgtcctgcac gtcctcgaag ctggttatcg atacgacgag attcatgtcg
     5701 ttctgtcccg ataactgcta gaccaccaag ttctcttacc ccaagtccta acttgatatc
     5761 tgttccccga ccagccatgt tggtagcaat cgtcacagca cctttttgac cagcattcat
     5821 gatgatctct gcttctttaa agtgattttt cgcattcagc acttcatgag gaatacgttc
     5881 ttgattcagc atatctgata ataactcaga tgtttcaacc gcaacagttc cgactaacac
     5941 aggttgacct ttgcggtagc gatctttgat atcttgcacg acagcgtgga acttgctttg
     6001 taatgttggg taaagcaaat ctggacgatc atcacggata accggacgat ttgtcggaat
     6061 ctggtatact tggatgttat aaatttctct gaattcttct tcttctgttt tagctgtacc
     6121 agtcattccc gcaagtttct tgtacatacg gaaatagttc tggaaagtga tcgtcgccat
     6181 ggttttcgtt tcgtcttcga tctctacgcc ttctttagct tcgattgctt ggtgaagtcc
     6241 atctgaataa cggcgtccat ccatgatccg accagtgaac tggtcaacaa tcagtacttt
     6301 tccgtcttgg acaacataat cgatatctaa aagcatgata tagttggctc tcaatgcttg
     6361 atccaagtga tgggtcaatg cagtattttc aatgtcgtat aagttatcca aaccgaatgt
     6421 ttcttctgct ttttcgatcc ctgcttcggt taaactgatt gtttttgatt gtacatcgat
     6481 tttataatct tcttcttctt tcaaacgttt cacgaagttg tctgctcttg tatataaagc
     6541 tgtggatttt tctgcttgcc ctgaaatgat cagcggcgtt ctcgcttcat caatcaagat
     6601 tgagtcaact tcatcgacaa tggcataatt cagcggacgt tgtaccattt gatggcggta
     6661 aacgaccata ttgtctctca agtagtcaaa tcctaattca ttattcgtac tataagtgat
     6721 atcacaatta taagcctcgc gtttttcatc cgaagatttt gagttgatat tcaaccccac
     6781 tgttaatcct aagaaattat aaagttcgcc catttccgtc gagtcacggg ttgctaaata
     6841 ttcattgacg gtaactacgt gtaccccttc gccagtcaga gcatttagat atactggcat
     6901 cgttgcggtc aaggttttac cttcaccagt acgcatttca gggatatttc catcgtgaag
     6961 cacgattcca cccatcaatt gtacgtggta tggatataaa cctaatacac gttttgctgc
     7021 ttctcggacg actgcaaaag cttccggaag taattgatct aatgtttctc ctttttggta
     7081 tctgcccttg aattcagcag tttttgcctg taattgttca tcagttaatg ctgccatttg
     7141 gtcagcgtat gcttcaactt tttttgcaat gctatctaaa cgttttagtt ctttcttatc
     7201 gttttcgata agtgaacgaa tcaaattggc catgcgttgt gtaatctcct tttccttctg
     7261 tgtattatcc agactatgta gactttactt ttcattttat cattagttat agataattga
     7321 aatatcttaa gcgtattaaa gtaagaactt cataatttat tcatatttca tctaaaagag
     7381 gaggaaactc
//
LOCUS       DASHHB010000096         6412 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.100, whole genome shotgun sequence.
ACCESSION   DASHHB010000096 DASHHB010000000
VERSION     DASHHB010000096.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6412)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6412)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6412
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.100"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            76..1380
                     /locus_tag="U2K71_002643"
     CDS             76..1380
                     /locus_tag="U2K71_002643"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding protein"
                     /protein_id="HEM7316656.1"
                     /translation="MLNDINNIKKEFEEQIAYTHREKDRYTLYFSEHIPRCKIMQQLS
                     QNSLFLKTCLLYLEEDEPDYLQLTECEFISVSKAYSLKKQVLAYFNDCGIEIDRYSPR
                     FTEMERRLLLLNVSYRLGGFNSWELPESFFERADRFIESVTENSGRFYDKENKEILLI
                     GFAISFLRQQVCAVTIDSKFIEEIKKRPIYNYVESAWENTDFQTYYKKEEFAFILTLF
                     NLCNYGFHSYQLIAEDFQQLHQVFIDNTPEIKELVATFESHFNQELFGNQPFERALIH
                     LMRSAWDNYQLFMPEKFYLLNEEQTNLYKEVQTIFSSWSSQLPYDLRLNPNCMRAFVI
                     ELSGILRLTKEHLTIYIVTNSDVHYLIYREALEAVTTFDFQVAPTIYSSISDIKKYAQ
                     QSSNRVLCERTLYTPDAVQYENIIPISINTIDRAIISAVQNK"
     gene            complement(1472..1837)
                     /locus_tag="U2K71_002644"
     CDS             complement(1472..1837)
                     /locus_tag="U2K71_002644"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEM7316657.1"
                     /translation="MTTELMQFLLPYFEKLVLGLDIFAICLLVWGVCLSIKDFLKYLF
                     VHTKVQSDRVSENNAIKKMLGGYILLSLEILVSAGIIESIIKPTLQDIFQLAALVVIR
                     TIISYFLNKEIGPVDVQNK"
     gene            complement(1834..2199)
                     /locus_tag="U2K71_002645"
     CDS             complement(1834..2199)
                     /locus_tag="U2K71_002645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1622 domain-containing protein"
                     /protein_id="HEM7316658.1"
                     /translation="MFRSNLIEFLEWIIAILNICSIGILIVGIVLVLFKLFDWSHVKK
                     NYAERNTRNADTRKLLASYILLSLEVLVVADIIESVIKPTWTDILKLALIIIIRTVIS
                     YFLNQEIEDKEKQRRGEVG"
     gene            complement(2391..2639)
                     /locus_tag="U2K71_002646"
     CDS             complement(2391..2639)
                     /locus_tag="U2K71_002646"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316659.1"
                     /translation="MKNASLFSLSISKDSSFLLECQMIGREEKGNFDKKEEVSDFPVN
                     KNRVGKKKRFEEVYGSIFRNLGTKKAPRQCFFEKLMTD"
     gene            2638..2766
                     /locus_tag="U2K71_002647"
     CDS             2638..2766
                     /locus_tag="U2K71_002647"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019724379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methanol dehydrogenase"
                     /protein_id="HEM7316660.1"
                     /translation="MSKKLIKVGIGLGLLALGAAYLGKKTGLFEDDSHLYDEFESI"
     gene            2823..4517
                     /locus_tag="U2K71_002648"
     CDS             2823..4517
                     /locus_tag="U2K71_002648"
                     /EC_number="4.2.1.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oleate hydratase"
                     /protein_id="HEM7316661.1"
                     /translation="MRKSKAIMIGAGIANMAAAVYLIQEGKWKGDQITFYSLDDHGSN
                     DGAPTDTVVDEYWNKNHPLENQKGYIARGGRMLNYRTYVDLMDLLSRIPSVTEPGMTA
                     EEDTRDFDAKHRTFDKARLMEGGKGIIDGGKLGFNNKDRILLTKLIAMPDSEEEKLDN
                     ITIEEYFKDDPHFFETNFWFMWETTFAFRTRSSAQELRRYMHQMIYEFTQIEHLVGVN
                     RTRYNQYESIMLPLIEYLKEQGCKIILNRRVTDWEFKETPMQDEITVTGLKMTNTLTQ
                     EEEHVVVDEDTAVIFTNGSITDSATLGDYETPAVENMDYGAASSLWKKASERFYNLGN
                     PDKFFADRDASEWVSFTLTTKNHLLLNEITRITTQEPGNALNSFISTTPITPLGQKDV
                     NMSIVVHHQPHFTSQKPNETVIWGYFLYPRRRGEFVDKQYIKMNGKEMLEELIGQLSK
                     VDPGPVNIREKETEIFDSVINNIPVYMPYASALFNNRAKSDRPKVIPKHSTNLAFTGE
                     FVEQPYQMIFTEQSAVRSGEIAAFHFAGIPMSRLVKTPRYDKDIPTLARAAKKMFE"
     gene            4521..4640
                     /locus_tag="U2K71_002649"
                     /pseudo
     CDS             4521..4640
                     /locus_tag="U2K71_002649"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349726.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
     gene            4617..4790
                     /locus_tag="U2K71_002650"
     CDS             4617..4790
                     /locus_tag="U2K71_002650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316662.1"
                     /translation="MQAFEHRLPRGCEKAALHQVIRAAKLKQLLMFLTWLDLLPQMQA
                     FEHRLPRGYEKVV"
     gene            5168..6301
                     /locus_tag="U2K71_002651"
     CDS             5168..6301
                     /locus_tag="U2K71_002651"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010736480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase catalytic
                     domain-containing protein"
                     /protein_id="HEM7316663.1"
                     /translation="MKAVTWQGKQKMEIRNVDDPKILEPTDAIIRITATAICGSDLHL
                     YHHGEAVLEKGYVVGHEPMGIVEEVGSQVQTLKKGQRVVIPFNISCGHCHFCLNHMES
                     QCDESNPDQLYGGLFGFGKLNGNHWGGQAEYLRVPYADSTSFVVPDNDLPDEKVLFLS
                     DILPTAYWSVENAGVKKGDTVVILGSGPVGLFAQKFAVMAGAERVIAVDPVQHRLDKA
                     ANYNGVETYFLEDTAQAGTELYELAKGGADVIIDCVGMDGLEPVKEKAKNLVSLQNGT
                     ISPLQMASQAVKKFGTVQVTGVYMTPASSYPLQEFFMRDIAVKHGQAPIIHLMPKIYE
                     MIAEGLFDPTQIITHSLPLSEAAQAYEIFDKKEDKNIKVVLKP"
BASE COUNT         2121 a         1223 c         1169 g         1899 t
ORIGIN      
        1 tgtgggcaat tctatgtaaa taaagaagtc aatctagaaa agctctccaa tctcctgcat
       61 gtttgcaaaa ccactttgct caatgatatc aataacataa aaaaagaatt tgaagaacag
      121 atcgcttata cacatcggga aaaggaccgt tacactttat atttttcaga acatatacct
      181 cgatgcaaga tcatgcagca attgtctcaa aattcgttgt ttttaaaaac ttgtctgctt
      241 tatttggaag aagatgaacc tgattatctt caattaactg aatgtgaatt catctctgtc
      301 agcaaagctt attcattaaa aaaacaagta ttagcttatt ttaatgactg cgggatcgaa
      361 atcgaccgct actcccctcg attcactgaa atggaaagac gtctgctgct tcttaatgtg
      421 tcgtatcgtt taggcggttt taattcttgg gagctacctg aatcgttttt tgaacgtgcc
      481 gatcgtttca ttgaatccgt aacggaaaat agtggcagat tttatgataa agaaaataaa
      541 gaaatcttat tgattggttt tgctatcagt ttcctacgcc agcaagtgtg tgctgtgaca
      601 atagattcaa aatttatcga agaaatcaaa aaacgtccta tttacaatta tgtcgaatca
      661 gcatgggaaa acactgattt ccaaacctat tataaaaaag aagaattcgc ctttattttg
      721 acactattca atctttgtaa ttatggtttc cattcttatc agttgattgc ggaagacttt
      781 caacagctac accaagtgtt tattgacaat actcccgaaa taaaagaact agttgctacc
      841 tttgaatctc acttcaatca agaattattc ggaaaccaac cattcgaacg tgcgctgatt
      901 catctgatgc gttcggcttg ggataactat caattattca tgccagaaaa attttatctt
      961 ctaaatgagg aacagacaaa tttatataaa gaagtccaga caattttttc cagctggtcc
     1021 agccagttac cttatgatct acggttgaat cctaattgca tgcgggcttt tgtgatcgaa
     1081 ttgagcggaa ttctccgtct gacaaaagaa catctgacta tctatatcgt cacaaattcg
     1141 gatgttcatt atctgattta tcgggaagcc ttggaagctg taactacttt cgattttcaa
     1201 gttgcaccaa caatttattc aagtatatct gatataaaga agtatgcgca gcagtcctca
     1261 aaccgggtat tatgtgaacg tacattatat acaccagatg cggtccaata tgaaaatatt
     1321 attcctattt cgatcaatac gatcgatcga gctatcattt cagctgtgca aaacaaataa
     1381 gggactgtga caaaaatcat gtcacagcct cttatttgtt gaagatgccc atttctgtaa
     1441 caatctcttg atacacatca ggctcacaac ctcacttatt ttggacatct acgggtccga
     1501 tctctttatt taaaaaataa gaaatgatgg ttcggataac taccaacgcc gctagttgga
     1561 aaatatcctg caaggtaggt ttgataatcg attcgatgat cccagctgaa acaagaattt
     1621 ccaaacttag aagtatatag cctcccaaca tctttttaat agcattgttc tcgctgactc
     1681 ggtcgctttg cacttttgta tggacaaata aatatttcaa aaaatccttg attgatagac
     1741 aaacacccca aaccaataaa cagatagcaa agatatcaag tcccagtaca agtttttcaa
     1801 aatatggaag aagaaactgc atcaactctg tcgtcatcct acctctcctc ttctttgttt
     1861 ttctttgtct tctatttcct gattcaagaa ataagaaatg actgttcgaa tgataataat
     1921 aagtgcaagc ttcaaaatat ctgtccaagt cggcttgata actgactcga tgatatcagc
     1981 aacgactagt acttccaagc ttaacaaaat ataacttgct agcagttttc ttgtatctgc
     2041 attacgagta ttacgttctg catagttttt cttgacatga gaccaatcaa aaagtttgaa
     2101 tagaacaaga acgatcccga caattaagat accgatagaa caaatattta aaatagcaat
     2161 gatccattct agaaattcaa ttaaatttga gcgaaacata tacgctacct actttctttt
     2221 aatttgttcc tattctttta ttgtaaaaga aagttacctt gttcgtctat ttaaaagttg
     2281 ttctaatgat aacggttcgc ttaaattttt ctttttcact ctcccctttt tctccctcca
     2341 tccataaatc gtggaagtat ccaaaacaac gtagcaagtt aaaataaggc ttagtccgtc
     2401 atcagctttt caaagaagca ctgtctcggt gctttttttg tacccaaatt tctaaaaata
     2461 gaaccataca cctcctcaaa tcttttcttc ttacctaccc tatttttatt tacaggaaaa
     2521 tcagacactt cttctttttt gtcaaaattt cctttttctt ctcgtcctat catttggcat
     2581 tctaataaaa acgaactatc cttggatata gaaagagaaa acaaggaggc gtttttcatg
     2641 agtaaaaaat taatcaaagt cggcatcggt ttaggtttgc ttgcccttgg cgctgcatat
     2701 ctaggaaaaa agacaggact attcgaagat gacagtcatt tatatgatga attcgaatca
     2761 atctagcatc ctatttagtt gaatcactac ggacatactt ggaagacatt ggaggaatca
     2821 caatgagaaa aagtaaagca ataatgattg gtgccggtat tgccaatatg gcagcggctg
     2881 tttatttgat tcaagaaggc aaatggaaag gggatcagat caccttttat tcattggatg
     2941 atcacggttc aaatgatggt gcaccgaccg acactgtagt ggatgaatat tggaataaaa
     3001 accatccatt agaaaaccaa aaaggctata ttgctcgagg cggtcgcatg cttaattacc
     3061 gtacctatgt agatttgatg gatctgttaa gtcgaatacc ttctgttact gagccaggga
     3121 tgactgctga agaagatacg agagattttg atgcaaaaca ccgaaccttt gacaaagcac
     3181 gtttgatgga aggtggcaaa ggaatcattg atggcggaaa actgggattc aataataaag
     3241 accgtattct tctgactaaa ctgattgcta tgcctgattc agaagaagaa aaactagaca
     3301 atatcactat cgaagaatat ttcaaagatg atccacactt ttttgaaaca aacttctggt
     3361 ttatgtggga aacaacattt gctttcagaa cacgtagttc tgctcaagaa ttgcgtcgct
     3421 atatgcatca aatgatttat gaattcactc aaatcgaaca tctagtgggc gtcaatcgaa
     3481 cacgttacaa tcaatacgaa agtattatgc ttcctttgat cgagtacttg aaagaacaag
     3541 gatgcaaaat cattttgaac cgccgtgtca ctgactggga attcaaagaa acaccgatgc
     3601 aagatgaaat cactgtgact ggtttgaaaa tgaccaatac actgactcaa gaagaagaac
     3661 acgttgtggt cgatgaagat acagcagtta ttttcactaa tgggtccatt acagattcag
     3721 ctactttagg agattacgaa acaccagctg tagaaaatat ggattatggt gcagcttcta
     3781 gtctctggaa aaaagccagt gaacgtttct acaacttggg aaatcctgac aaattctttg
     3841 ctgatcgaga tgcaagcgaa tgggtcagct ttaccctgac gacgaaaaac catttgttgt
     3901 tgaatgaaat cacccgtatc acgactcaag aaccaggaaa tgccttgaat tcatttattt
     3961 caaccacacc gatcacgccg ttaggccaaa aagatgtcaa tatgtctatc gtcgtgcatc
     4021 atcaaccgca ttttacgtct cagaaaccaa atgaaacagt tatttggggt tatttccttt
     4081 atccaagacg acgtggcgaa tttgtggata aacagtatat taaaatgaat ggaaaagaaa
     4141 tgctagaaga actgattgga caactttcaa aagtagatcc tggaccagtg aatattcgtg
     4201 aaaaagaaac agaaattttc gacagcgtaa taaataatat ccctgtctat atgccttatg
     4261 cttccgcgtt gttcaataat cgtgccaaaa gcgatcgacc aaaagtcatt cctaaacatt
     4321 cgaccaatct ggcctttaca ggagaatttg tcgagcagcc ttatcagatg atctttactg
     4381 aacaaagtgc tgtacgttct ggagaaatcg ctgctttcca ttttgcgggt atccctatgt
     4441 cacgtcttgt caaaacaccg cgttacgaca aagatattcc tactctagct cgtgccgcaa
     4501 aaaaaatgtt tgaataatca agaggttgtg aaaaagctgc cctgcatcaa gtaataagaa
     4561 caaccaggct aaaacagctt cttatgtttt tgatttggtt ggacttatta ccgcagatgc
     4621 aagcttttga acaccgttta ccaagaggtt gtgaaaaagc tgccctgcat caagtaataa
     4681 gagcagccaa gctaaaacag cttcttatgt ttttgacttg gttggactta ttaccgcaga
     4741 tgcaagcttt tgaacaccgt ttaccaagag gttatgaaaa agtcgtttag tttcgagtat
     4801 gaagaaaaat tttgtgaaaa tggttcctca cgtttttgca taaatttgac ttaataccga
     4861 gaaactgatc ccttctgtca taacctcttt ttatttatgt ctgtttcttt ttgaaaacaa
     4921 ccattttttc aacgtttact ctcttgccaa tccaacatca tctaacagat acaactattt
     4981 caatgattac cattacacta aaaaatcgga aactcacctc tccgaacaca aatacataag
     5041 actatagaat gtttcattta agaaaaaaag cgtttcatta tcaaaaaggt aagatctcct
     5101 ttaaaatgaa attatctgta gtactaataa tgcagacaat tttatgaatc tgaaaggagc
     5161 atctccaatg aaagcagtca cttggcaagg aaaacagaaa atggaaatca gaaacgtaga
     5221 tgatccaaaa atattagaac caacagatgc aatcatccgt atcacagcaa cagccatttg
     5281 cggctccgat ctgcatcttt accatcatgg tgaagcagtt ttggaaaaag ggtatgttgt
     5341 aggacacgaa ccaatgggaa tcgttgaaga agtgggatct caagttcaaa cattgaaaaa
     5401 agggcaacgt gtggttatcc cttttaatat tagttgtggt cattgtcatt tctgcctgaa
     5461 tcacatggaa agccaatgtg atgaatcaaa tccagatcag ttatatggcg ggcttttcgg
     5521 atttggcaag ttgaacggta atcattgggg tggtcaagcg gaatatctaa gagttcctta
     5581 tgcagactct acttcatttg ttgtaccaga taatgatctt cctgatgaaa aagttttatt
     5641 tctatcagat attttgccca cagcttattg gagcgttgaa aacgcaggag taaaaaaagg
     5701 cgatacagtt gtgattctag gttctggtcc agtcggtcta ttcgctcaaa aattcgctgt
     5761 tatggctgga gcagagcgcg taatcgcagt agatccagta cagcaccgtc tagataaagc
     5821 ggctaattac aatggggttg aaacttattt cttagaagac acggcacaag ctggaacaga
     5881 attgtatgaa ctggctaaag gtggcgcaga tgtcattatc gattgtgtag gaatggacgg
     5941 attagagcct gtcaaagaaa aagcgaaaaa tttagtaagc ttacaaaacg gcacaatctc
     6001 tccattacag atggcatcgc aagctgtcaa aaaatttgga acagtccaag taactggtgt
     6061 ttatatgact ccagcttctt cttacccgct gcaagaattt tttatgcggg atatcgctgt
     6121 caagcatgga caggcaccaa ttatccatct aatgccaaaa atttacgaaa tgattgcaga
     6181 aggactattc gatcctacac aaatcattac tcattcgctg ccactatcag aagcagcaca
     6241 agcatatgag atctttgaca aaaaagaaga taaaaatata aaagttgttt tgaaaccttg
     6301 aattcagtaa aaaatacagg tgattttctt tatgcccaaa atcaaacgaa atacccatga
     6361 gatcgaaata ggaaaagaag aagatatcga aatacgagga cgtcgcttcc gc
//
LOCUS       DASHHB010000097         6386 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.101, whole genome shotgun sequence.
ACCESSION   DASHHB010000097 DASHHB010000000
VERSION     DASHHB010000097.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6386)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6386)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6386
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.101"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            330..1175
                     /locus_tag="U2K71_002652"
     CDS             330..1175
                     /locus_tag="U2K71_002652"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM7316664.1"
                     /translation="MDEMKLIGIDLDGTLLNSDQSISQKNARTMKHLPEDCFPFICSG
                     REVEDINNILKKSGLRIPAVGLNGSVGFDGEKKIFESSFDKNDVKKIDSVLSKFPTKI
                     YTNLGSYESQNYKNKMKKIFHEVGSEFSIKELNYELEYEKSICSIPYNNIEEIIEKDA
                     ISIYKFFIFIPNRQLKANIFNHLKKMLNVSVTESAPVNIEIVPQNVSKGVVFDRLESI
                     YNLKKPLRIAIGDSLNDLSMFESADISFAMGNSHQEIMKKADYVTVSNDKDGVSQAFE
                     RILTI"
     gene            1456..2676
                     /locus_tag="U2K71_002653"
     CDS             1456..2676
                     /locus_tag="U2K71_002653"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair exonuclease"
                     /protein_id="HEM7316665.1"
                     /translation="MVKFIHAADLHMDRSFEGLTTLDKTVQEKLLKTNLTVLSNIIEQ
                     AIINNVDFVLLAGDNFHQNRPSLKIQKHFSEQMKRLEKNHIPVFIIFGNHDFYQKERY
                     WFSFPKNVHLFTSETVETKKITIKSGETVSLSGFSYRQPWIQKDKVMEFPSRELTDYH
                     IGLYHGEPGVSQKGNYAPFQPSKMQEKGYDYWALGHIHVPTVLNEKQTIVYPGAPQGH
                     TKKEQASTSILLVELSKGSCQIHPIKVAEVYWKTKEISLRKARTTKEVIVHIRQQLSK
                     VEDGFSLIEIKLKDYDHLNTDVLERIQSGELLDYLLEEFSDRINDFFIWRISLIENTF
                     YTKTPLAASAKLMEQLFQYYQTPQDFQQILNEVYSHQEAARILSELPEYQEETLEKAK
                     QLLNQDFLFEEDQE"
     gene            2673..5393
                     /locus_tag="U2K71_002654"
     CDS             2673..5393
                     /locus_tag="U2K71_002654"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM7316666.1"
                     /translation="MKILRIEIAAFGKWRQKSFDFYSGNQLIYGGNEAGKSTIYQFIQ
                     AILFGFPSKGRKKKDYTPKDGSAYGGKIWLKHPVYGEFAVERYKQQNRGKSKVWLGDQ
                     VGSDELLEKLIAPLTKELFQQVFTFQQEQLMELDHLREKELHDALISLGITGSSQVFQ
                     KRQEYYQKAQQLFKKKGQKLLINQKLNEWKELQEKIHQKQEQEAYFGKLIQQEQEYES
                     QQQQLLAQLKEANKQFSLARQQELNFSLYEEWQSLKKNSTTGLPDEQLLLDLESFSKR
                     YQQTNERLEELEATLEKHSGMDQQSARYYFYLEQEQELKDLQQLQFTAEQLNEEWQRL
                     QIETRETNSELKILEQRWHWQKNPLPEEFLYEEEWKHLLAEERRIADLESQISVRLQI
                     AKEQQEMIEAEVNDLEETYPALLQNQSEIAPVSSSKPYMLMAGGAIIIAGIFVPMPLK
                     VILLLIGAGMIGYFFYKRQPKIKTKNDFSEIKGMWQEKLGQLDLALAQLAEAEEKQKR
                     TGEEKQILQQEVYRFADHRHLGKMDTLKLMKEYGQEVIDYQKLYGVFERKHKREQEIQ
                     EKLRQIDQKFEFVKEWLPIQDKTISEKMVLLSRFIQEMEQMKFARSYQQNTLLKQEIN
                     QLKKKQKVWIQEYSEQLTQAGLSYPSEVPVFLQKAKQAEQQMKRYNELSEMLTPLFPE
                     ELTKEKLSVRLRQLKEKQSQLQQEEKEASENRQRLQLQVEELQRDGTLDELYQQASRQ
                     KAKLQELLEEWGALEIAGTFLGDLSTEMSERQLPQLLRAAGDYLGILTKGNYQKILLK
                     NDTLTLTDGYTEFPIYELSTGTKDQLIMAMRFAYLSLEANRSICPIIIDDGWLHYDHQ
                     RKYQLAELLAKFGEKYQIICFSSDKEMVSYYHEMNQIVKQLEGANNEKNS"
     gene            5377..6321
                     /locus_tag="U2K71_002655"
     CDS             5377..6321
                     /locus_tag="U2K71_002655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HEM7316667.1"
                     /translation="MKKIRDLVIDEQFEQFVLIKSADVRIAKNGKKFIAFTFQDTSGT
                     IDGKYWDASEEEINKFTAGTVVFLNGKREVYQGNPQVKILHMRLTRVDEPGEPSLYME
                     RAPLKKEEMEEEINQLLFEITNAHWNRIVRYLLNKYQKQFFEFPAAKRNHHAFASGLA
                     YHTLTMLHLGKSIADEYPELNRALLYAGIILHDLGKVIELSGPMSTEYTLAGNLIGHL
                     VLIDEEITKACLALKISEADEDVLVLRHMVLAHHGLLEYGSPVRPRIMEAEILHQIDN
                     IDASMQMMLTSIRQTEPGKYTDRIFGMDNRSFYVPKDL"
BASE COUNT         2379 a          986 c         1231 g         1790 t
ORIGIN      
        1 aaggcaaaaa gatgaactag aaatagttca tctttttttg tttaaatctt ttgctaagtt
       61 gaactaatag agtcttttat gaatatcgaa gttagttttt cacctaactt cgttttttat
      121 agttggttat tataaaatag taatatagct gatttggatt caattcatat ttatttataa
      181 atcaaaaata cagagaatag tatataaatg tcctttccta ttcttaaaat gatgtttgta
      241 cttacttaaa ttaggtataa ttagtatatg ttttgtgaga aatttagact tacaataagc
      301 agtgaattta taatcaacgt ataggagaca tggatgaaat gaaactaatt ggtatagatt
      361 tagacggtac tcttttgaat tctgaccaga gcattagtca aaaaaatgca agaacaatga
      421 aacatttacc ggaagactgt tttccattca tttgttctgg aagagaagta gaagacatta
      481 ataatattct caaaaaatct ggtttaagga taccagcagt agggttaaat ggttctgttg
      541 gatttgatgg agaaaaaaag atatttgagt cttccttcga taaaaatgat gtaaaaaaaa
      601 ttgattctgt cctatcgaaa tttccaacca aaatatatac aaatctcgga agttatgaat
      661 ctcagaatta taaaaataaa atgaaaaaaa tttttcacga ggtagggagt gaattttcta
      721 tcaaagagct aaactatgag cttgagtatg aaaaatctat ttgttcaatc ccttataata
      781 atattgagga aattatagaa aaggatgcta tcagcattta caaatttttt atttttattc
      841 ctaatcgtca attgaaagct aatatattta accatttgaa aaagatgcta aatgtgtctg
      901 ttactgaatc ggcacctgta aatattgaaa tcgtacctca aaatgtatct aaaggtgtgg
      961 tgtttgatcg tttggaatca atctacaatt taaagaaacc tttgagaatc gcgatcggag
     1021 atagtttaaa tgacttatct atgtttgaaa gtgcagatat tagttttgca atgggaaata
     1081 gtcatcaaga aatcatgaaa aaagctgatt atgtcactgt ttcaaatgat aaagacggag
     1141 tatctcaagc atttgaaaga attcttacca tataaactaa ttaaggtagt atagaacata
     1201 gaattctaaa tacggaaaaa tggagcataa attccagtac aaatagtact tcttcagtgt
     1261 caaacgaagc gaatataaca tagatattta ggattgtgac aaaaatcatg tcacaatctt
     1321 tttttcgaat aaacttattt tttgaagtcg gccatttatc gtaatttctt tgcataatac
     1381 gaacaaattt tcggttattg atgaaaaaaa acattgaaaa tggtatgata aaagaaaaaa
     1441 aggaggaaat ttttaatggt gaaatttatc catgcggcag atttacatat ggatcggtca
     1501 tttgaaggac tgacaactct tgataaaact gtacaggaaa aactattgaa gacgaatctg
     1561 accgttttat caaatatcat tgaacaggca ataataaata acgttgattt tgttttactt
     1621 gcaggagata attttcatca aaatcgtcct tcattaaaaa tacagaaaca tttttcagaa
     1681 cagatgaaac ggttagaaaa gaaccatata cctgtattta taatattcgg aaatcatgat
     1741 ttctatcaaa aagagcggta ttggttctcc tttcctaaaa atgtccattt atttacatcc
     1801 gaaacggtag agacaaaaaa aataacgata aaaagtggag aaactgtctc tctcagtgga
     1861 tttagttatc gtcaaccgtg gattcaaaaa gacaaagtga tggaatttcc ttctagagaa
     1921 ctcacagatt accatattgg gttgtaccac ggggaacctg gcgtctctca aaaaggaaac
     1981 tatgctcctt tccaaccctc aaagatgcaa gaaaagggat acgattattg ggctttaggg
     2041 catatccatg tgccaacggt cttaaatgaa aagcaaacaa ttgtttatcc aggtgcgcct
     2101 caaggtcata ctaaaaagga acaagcttct acatctattc tactagttga attaagtaaa
     2161 ggaagttgcc aaatacatcc gattaaagta gcagaggtct attggaagac aaaggaaata
     2221 tcattgagga aagcaagaac gacaaaagag gtcattgttc acattcgtca gcagctttca
     2281 aaagtagagg atggatttag tttaattgag ataaaattaa aagattatga ccatctcaat
     2341 acagatgttt tggaacggat acaatcagga gagctattag actatttgtt agaggaattc
     2401 tcagatagga taaatgattt ctttatttgg cgtatttctc tcatagagaa tactttttat
     2461 acgaagaccc cacttgctgc ttctgctaaa ttgatggaac agttatttca atactatcag
     2521 acacctcaag atttccaaca aattttaaat gaagtatata gtcatcaaga agcggctaga
     2581 atattgagcg aactgccgga atatcaagaa gaaacacttg aaaaagccaa gcagctgctt
     2641 aatcaagatt ttttatttga ggaggatcag gaatgaagat tttaaggatc gagattgctg
     2701 cttttggaaa atggcgtcaa aaaagttttg acttttattc aggcaatcag ctgatctatg
     2761 ggggaaacga agcaggaaaa tcaacgattt atcaatttat tcaagcaata ttatttggtt
     2821 tcccttcaaa aggccgtaaa aaaaaggatt atacaccaaa agacggctca gcctatggcg
     2881 gcaaaatatg gcttaaacat cctgtatatg gtgaatttgc tgtcgagcgc tacaagcagc
     2941 aaaatcgcgg aaaatcgaag gtgtggctgg gggatcaagt gggaagtgat gagttactag
     3001 aaaaactgat cgcacctttg acgaaagagt tattccagca ggtatttact ttccagcaag
     3061 agcagttgat ggaactagat catttgagag aaaaagagct acatgatgcg ctgatttctt
     3121 taggcatcac gggcagttct caagtattcc aaaagcggca agaatattat cagaaagcac
     3181 aacaattatt caagaaaaaa ggccaaaaat tactaatcaa tcaaaaatta aatgaatgga
     3241 aagagctgca agaaaaaatt catcaaaaac aagaacagga agcttatttt ggcaagttga
     3301 tccaacaaga acaggaatac gagtcgcaac agcagcagtt attggcacaa ttaaaagagg
     3361 cgaacaaaca attttcatta gccagacagc aagaacttaa tttttcttta tatgaagaat
     3421 ggcaatctct caaaaaaaac agcactacag gtcttcctga tgagcaactt cttttagatc
     3481 tggagtcttt ctccaaacgg tatcagcaaa caaatgagcg tttggaagaa ttagaagcaa
     3541 cactggaaaa gcatagcggg atggatcagc agtcagccag atattatttt tatttagagc
     3601 aggagcagga actaaaagat ctccagcaac ttcaatttac agcggaacaa ttaaacgaag
     3661 aatggcaaag attacaaatt gagactagag aaacaaattc agaattgaag attttggaac
     3721 aaaggtggca ttggcagaaa aatccgttac cggaagaatt tttatatgaa gaagaatgga
     3781 agcatcttct ggctgaagaa agaaggattg ctgatctaga aagccagatt tctgtgcgtc
     3841 tccaaatagc taaagaacag caagagatga ttgaagcaga agtgaatgac ttggaagaga
     3901 cctatccagc attattacaa aatcagtcag agatagcacc agtctcttca tctaaaccgt
     3961 atatgctgat ggctggcgga gcaataatca ttgcaggtat atttgtacca atgcccttga
     4021 aagtgatcct tttactaatt ggtgcaggca tgattggcta tttcttctac aaaaggcaac
     4081 caaaaattaa aactaaaaat gatttctctg agataaaagg aatgtggcaa gaaaaactag
     4141 gacaacttga tctagcctta gctcaattag ctgaagcaga agaaaaacaa aagagaactg
     4201 gagaagaaaa acagattctt cagcaagaag tatatcgttt tgcggatcat cggcacttag
     4261 gaaagatgga tacgctcaaa ctcatgaaag aatatggaca ggaagtcata gattatcaga
     4321 aattatatgg cgtatttgag cggaaacata aaagagaaca ggaaatacaa gaaaaactac
     4381 gacaaatcga ccaaaaattt gagtttgtta aagaatggtt accgatacaa gataagacaa
     4441 tttcagaaaa aatggtattg ctttcccgtt ttatacaaga gatggaacag atgaaatttg
     4501 ccagaagcta tcagcaaaat actttattga agcaagaaat caatcaattg aaaaagaaac
     4561 aaaaagtatg gatacaagaa tacagcgaac aattgactca agcaggctta tcttatcctt
     4621 cggaagtacc tgtcttttta caaaaagcca aacaagccga acagcagatg aaaagataca
     4681 atgaattatc ggaaatgctt actccactat ttcctgaaga gctgacaaaa gagaaattat
     4741 ctgtaagact gcgccaactg aaagaaaagc aatcccaact ccagcaagaa gaaaaagaag
     4801 caagtgaaaa tcggcaacga ctccaacttc aagtagaaga gttgcaaaga gacggcacat
     4861 tggacgaact ttatcaacaa gctagccggc aaaaagcaaa attacaggaa ttactggagg
     4921 aatggggagc acttgaaatc gccgggacat tcttaggtga tctttcaaca gagatgagtg
     4981 aaagacaatt accccagttg ttaagagcag caggtgatta tttaggtatt ttaacaaaag
     5041 gaaattatca gaagatatta ctgaaaaatg atacactgac tttaacagac ggatatacag
     5101 aatttcccat ctatgagtta tctacaggta cgaaagatca gctgattatg gcgatgagat
     5161 ttgcttacct ctcattagaa gctaaccgtt cgatatgccc gatcatcatt gatgatggct
     5221 ggcttcatta tgatcatcag cgaaagtatc aattagctga acttttagct aaatttggag
     5281 aaaagtatca aataatttgt ttttcttctg ataaagaaat ggtaagctat taccatgaga
     5341 tgaaccaaat cgtaaaacaa ttagaaggag caaataatga aaaaaattcg tgatttagta
     5401 atcgatgagc agtttgaaca atttgtcctg atcaaaagtg cagatgtaag aatcgctaaa
     5461 aacggaaaaa aatttattgc ttttactttc caagatactt caggtactat cgatggtaaa
     5521 tactgggatg cttcggagga ggaaatcaat aaatttacag ccggcacagt cgtttttcta
     5581 aacggtaaaa gagaggttta tcaaggaaac cctcaagtaa agattcttca tatgcgctta
     5641 acaagggtag atgagccagg agagcctagt ttatacatgg aaagagcccc tttgaagaaa
     5701 gaagaaatgg aagaagaaat caatcaattg ttgtttgaga tcaccaatgc gcattggaat
     5761 cgtattgttc gctatctact taataaatat caaaaacaat tttttgagtt tccggcagca
     5821 aaaagaaacc atcatgcatt tgccagtgga ttagcttatc atactttgac aatgcttcac
     5881 ttagggaaat caatcgccga tgaatatcct gagctgaatc gagcattgtt gtatgctggt
     5941 atcattttgc atgatttagg aaaggtgatt gaactttccg gtcctatgtc gacagaatat
     6001 acgcttgcag gaaatttgat cggtcatctg gtattgattg acgaagaaat aactaaagcc
     6061 tgtcttgcgt tgaaaatcag cgaagctgac gaagatgttc tcgtattacg tcatatggtt
     6121 ttagcacacc atggcttatt agaatatggc tcacctgtac gtccaagaat aatggaagca
     6181 gaaatattac atcaaatcga caatatcgat gcttcgatgc agatgatgtt gacttcgatt
     6241 cgtcaaacag aaccaggaaa atacaccgat cggattttcg gtatggacaa ccgaagtttc
     6301 tatgtaccaa aagatttata gcaaaaattt atttttttca ttcaaaacaa aaaaagcgag
     6361 acataagtct tcgctttttt tgtttt
//
LOCUS       DASHHB010000098         6173 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.102, whole genome shotgun sequence.
ACCESSION   DASHHB010000098 DASHHB010000000
VERSION     DASHHB010000098.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6173)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6173)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6173
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.102"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            725..811
                     /locus_tag="U2K71_002656"
     CDS             725..811
                     /locus_tag="U2K71_002656"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEM7316668.1"
                     /translation="MFELFICPLLIEVCVSLFSYWLNNRNKK"
     gene            complement(932..1621)
                     /locus_tag="U2K71_002657"
     CDS             complement(932..1621)
                     /locus_tag="U2K71_002657"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316669.1"
                     /translation="MAFYLPYLLIFASILGIIWLSYKAYQTRYSLKGSKIRFKRFLLP
                     GVIFTLSIVLSGLFGVLEGNKRISRSILLGDITQKYEAACDKKKKEQVLTQKMEEFTT
                     CYEEMNDIFVNQEKRLTDKNMEKLTRLYQNLPEKQQKEVQDNYERTKKDVQYFKDTQT
                     EEACYNLFSDTIPFSTSEQERKERQQSVTYERYKALLQQATNIQNPTKKETALNYLKS
                     VKEWLDQQQQN"
     gene            complement(1628..1906)
                     /locus_tag="U2K71_002658"
     CDS             complement(1628..1906)
                     /locus_tag="U2K71_002658"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316670.1"
                     /translation="MKEEQTTNKNYRSKTLQDIENEIKKQRAFLAPYTKERIQTTIDS
                     QVKKFHHALLNQRNKEQKKVIMQRSMDILAPLCCLIMTLLAFWLVWGE"
     gene            complement(1866..2780)
                     /locus_tag="U2K71_002659"
     CDS             complement(1866..2780)
                     /locus_tag="U2K71_002659"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="relaxase/mobilization nuclease domain-containing
                     protein"
                     /protein_id="HEM7316671.1"
                     /translation="MATVKVSRTTSCSRAINYAEPRATVKTGINCDIDYAKSEMKQIR
                     MLYGKDDRVQAHLLIQSFRPGEITAEKANQLGAEYAKKIAPDHQIAIYTHTDKDHIHN
                     HIVINSVNLETGNKFQAHGQAFLNKCYDLNDEICLEHDLTITERGKKPEKRTMSEIKL
                     KEKNEPVWKDEIRFAIDQTMKNKKTRTYNQFCDSLKTFGIHCLNRGKNFTYELIHKKK
                     KVRSNKLGKDYEKETILHELDRRERAYNQQRTNELRRARASIRETAKEVRREQRTLTT
                     EQPEIRPTRRNHDQHVERGADYQQELSF"
     gene            complement(2762..3142)
                     /locus_tag="U2K71_002660"
     CDS             complement(2762..3142)
                     /locus_tag="U2K71_002660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MobC family plasmid mobilization relaxosome
                     protein"
                     /protein_id="HEM7316672.1"
                     /translation="MSEQNQYLASNHSKKYTYRSEPKQISFRVSESEFAKLKQSAEAL
                     QMSVPAFVKAKAQNARLVTPKVAPDIAQAIARDLAKAGGNINQIAKWCNTHQHDVDPG
                     DAQRLSENLKIMQKELQKIWQQLK"
     gene            complement(3592..3879)
                     /locus_tag="U2K71_002661"
     CDS             complement(3592..3879)
                     /locus_tag="U2K71_002661"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEM7316673.1"
                     /translation="MDFTKEEKLLNAISKVYNEATIDDYPDLKEKLFLYSKEISEGKS
                     VGEVSMKLSSFLGRYILKHKFGLPKSLIELQEIVSKESQVYRGWASIGIWS"
     gene            complement(3899..4123)
                     /locus_tag="U2K71_002662"
     CDS             complement(3899..4123)
                     /locus_tag="U2K71_002662"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin hiracin-JM79"
                     /protein_id="HEM7316674.1"
                     /translation="MKKKVLKHCVILGILGTCLAGIGTGIKVDAATYYGNGLYCNKEK
                     CWVDWNQAKGEIGKIIVNGWVNHGPWAPRR"
     gene            complement(4578..4748)
                     /locus_tag="U2K71_002663"
     CDS             complement(4578..4748)
                     /locus_tag="U2K71_002663"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316675.1"
                     /translation="MTKKHLLFGSILLGILSFSPMLLSVANKGNKKGELKLNPNYYNS
                     QNEEVFVEKLDK"
     gene            complement(4763..5296)
                     /locus_tag="U2K71_002664"
     CDS             complement(4763..5296)
                     /locus_tag="U2K71_002664"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEM7316676.1"
                     /translation="MQPQETFTIKELSDLFKMSRQAISKHIQKLDSSMIAKNERGYKV
                     VLRSGVLQLARNLDNQRLLDKLHKHEQNNDKNAYIPNKNVVANKLLDQLREENEFLRE
                     QLALKEQNNSHIQKLLDQQQQLTLQANQQIEKLQEQLQLTYTEESSENSSTTLSEATE
                     TMDEQTKNWKKWWQLWK"
     gene            complement(5317..6159)
                     /locus_tag="U2K71_002665"
     CDS             complement(5317..6159)
                     /locus_tag="U2K71_002665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEM7316677.1"
                     /translation="MREKGDKTVRFTFDQLKDLSNYKATANVRFVEDLKATYEKILSL
                     RFGRRSESGLSYDMFVMFTEFKINGDVENPYVDIRIYEKALPLLNNLDEWVRYSLQQF
                     NELQSGYAKTMFRLLKQYRTKGFAYFSKEDFLELLDIPKSYKQPDIDKRVIKPIRQEL
                     TAIFKGLTIKKKYGKGRGKPVIGYQFTFKPEIKKADDFNKHAPTKEDQNKALALADKQ
                     KEYEEPATTINEKIKQKKRELELLQQIKQLEKEKKALEKQTESALSEKEKKSLLSDLD
                     NLFQ"
BASE COUNT         1633 a         1151 c         1042 g         2347 t
ORIGIN      
        1 aaaaaaacaa attcatttca accggtgtaa attttcttaa aggaattgta tttaattcat
       61 ggtggtattt tacaatctca ttagccatat aatcacctca atccaattat gaatgacatt
      121 ttttaaatgt caagacattc atatttgacg ttcaaacaga caaaatgtcc ttcataaaca
      181 gacaaaatgt ccttcataaa cagacaaaat gtccttcata aacagacaaa atgtccttca
      241 taacattctg tatccattgg tagagtaagg acacagctta cggtaaagag gtttaaaaag
      301 aggtttaaaa agagatataa aaagaggggg gctaattttc ccgcctaaaa accacttctt
      361 ttttctttaa acaggttcgc gcatttttca aaactgccaa tccaaaacct tgcttcctac
      421 ggtcgccaaa aaggtgtgat cgctacgctc tcacattgta ctgcgcagtc gcctaaaggc
      481 tcctttgcta aaaacatgtt ggtaaagagg cctttgagcg gttctgaatg cttatctaag
      541 ccgttttaag acagtcttgg ataaaaatcc ttatctagct ttaaagcgcc ttacaggcgg
      601 tcagggcaag ccctaacaac cctaaaataa gcgatactta aaaacaccac acaagtttaa
      661 ttttttaaga tataataact tgtcgcctat ccgacagtct tgcgactgga aggaggtgta
      721 tctaatgttt gaattgttca tttgccccct tcttatagaa gtttgtgtct ctttattcag
      781 ttattggttg aataaccgaa acaagaagta aaatcaataa aggcgacatc aacctgaaaa
      841 aaagccccag agaatccgac ctctctgggg ctttcgtgta tctaatgttt gatcatttgc
      901 atgtttagtt taacatgatt gccgatcaaa ttcaattttg ctgttgctga tctaaccatt
      961 ccttgacact ttttagataa tttaatgccg tttctttttt cgttggattt tgtatgttcg
     1021 ttgcttgctg aagcagtgct ttataacgtt cgtaggtaac tgattgttgc cgttcttttc
     1081 gttcctgttc actagttgaa aaagggattg tatcactgaa taaattataa caagcttctt
     1141 ctgtctgcgt atctttaaaa tattgcacgt cttttttggt tcgctcatag ttgtcttgaa
     1201 cctctttttg ctgtttttct ggcaagtttt gataaagacg agtgagtttt tccatgtttt
     1261 tatccgtcaa acgtttttct tgattcacaa aaatatcgtt catttcctca taacaagtgg
     1321 taaactcttc cattttttgt gttaacactt gctccttttt ctttttatca catgctgctt
     1381 catacttttg agtgatatcc cctaataaaa tacttcttga aatacgttta tttccctcta
     1441 aaacgccgaa taaaccactt aaaacgattg ataatgtaaa tataacccct ggcaataaaa
     1501 acctcttaaa tcgaatttta gagcctttta atgaatatct tgtttgataa gccttatagc
     1561 taagccaaat aattcctaaa atactggcaa aaatgagtag atagggtaaa tagaatgcca
     1621 tttctattta ttctccccat accagccaaa aggctaacag tgtcatgatt aagcaacata
     1681 agggggcaag aatatccatt gatcgctgca taattacttt tttctgttct ttatttcgct
     1741 ggtttaatag cgcatggtgg aattttttga cttgtgaatc aattgtagtt tgtattcttt
     1801 ctttggtata aggtgcaaga aaagcacgtt gtttctttat ttcattttct atgtcttgta
     1861 acgttttaga acgataattc ttgttggtag tctgctcctc tttcaacgtg ttggtcatgg
     1921 tttcttcgag tcggtctaat ttccggttgt tcagtcgtaa gagttcgttg ttctcttcgt
     1981 acttcttttg cagtttcgcg aatgctagct cgagctcttc gtagctcgtt ggttcgttgt
     2041 tgattgtagg ctcgttctcg tctgtccagc tcatgtaaaa tcgtctcctt ttcgtaatcc
     2101 tttcctagtt tgttggatcg tacttttttc tttttgtgga tcaattcgta agtaaaattt
     2161 ttcccacgat tcaagcaatg aatgccgaat gttttaagag aatcacaaaa ttggttatac
     2221 gttcgagttt ttttgttttt cattgtttga tcaatcgcaa accgaatttc gtctttccaa
     2281 acaggttcat ttttttcttt taatttgatt tcagacatgg ttcgtttttc gggtttcttc
     2341 cctcgttcag tgattgttaa gtcatgttca agacaaatct cgtcatttag gtcataacat
     2401 ttgtttaaaa atgcttgacc atgggcttga aatttgttcc cagtttccag attgacggag
     2461 ttaatgacaa tgtggttatg aatatggtct ttgtctgtat gtgtgtaaat cgcgatttgg
     2521 tggtcagggg ctattttttt cgcatactct gcccctagtt gattggcttt ttcagcggta
     2581 atttctcctg gtcgaaaaga ctggatcaat aagtgtgctt gtacacgatc gtcttttcca
     2641 tacagcatgc gaatttgttt catttcgctt ttggcataat caatgtcaca gtttatccct
     2701 gttttgacgg ttgctcgtgg ttcggcgtaa ttgatcgctc ggctacaaga agtggttcgg
     2761 cttactttaa ctgttgccat atcttttgta gctccttttg catgattttt aggttttcgg
     2821 ataagcgttg ggcgtcgcca ggatcaacgt cgtgttggtg tgtgttgcac cacttggcaa
     2881 tttgattgat attcccgcca gctttggcta aatcgcgcgc gattgcctga gcaatgtctg
     2941 gcgccacttt cggtgtcacc agacgcgcgt tttgtgcctt ggctttcacg aaagccggca
     3001 cactcatttg caaagcttca gctgactgct tcagctttgc aaattcggat tcgctcactc
     3061 gaaaactgat ttgttttggc tcggatcgat aggtatattt tttagaatga ttgctagcca
     3121 aatattgatt ttgttcgctc ataagtccca ccctttcagt acgtcctacg gacgtatcgt
     3181 tcgcaatacc attgtaccta aaaaggtaca ataagtaaag ctcactaaag ctcactaaag
     3241 ggcttggcaa gctctatatt tgctagccaa atacctacgt atttggtgca aataaccgct
     3301 tgtcaaaggg gcgcaagctc ccccttgaat ccccctcccc cccactctcc acaatacaaa
     3361 aaagactagc aaatgctagc cactttttgt aaagtttcgc ttgtttcacc ttcggtgaaa
     3421 ttgttgcaaa tcaataatta ggtagagcta catttttttc gttgttcaaa gattcttatg
     3481 ccatttacta ttaataaatc tataaatatg ataatttttc ggctctataa tttatgggac
     3541 tgatgaatca gaattgattc ttcagtccca ttttcatttt attccaaaga attaactcca
     3601 aataccaata gaagcccatc ctctatatac ttgagattcc ttactaacaa tttcttgtaa
     3661 ttctattaaa gatttaggta atccaaattt atgttttaaa atatatcttc caagaaaact
     3721 acttaatttc atactaactt caccaacact ttttccctca ctgatttctt tagaataaag
     3781 aaagagcttt tcttttaagt caggatagtc atctatagtt gcttcattgt atactttact
     3841 aattgcattt aaaagttttt cttctttagt aaaatccatt ataataacct ccctaccact
     3901 accttctagg tgcccatgga ccatgattaa cccaaccatt aacaataatt tttccaattt
     3961 ctcctttagc ttgattccaa tctacccaac atttttcttt gttacaataa agaccatttc
     4021 catagtaagt agctgcatca acttttattc ctgtaccgat gccagctaga caagttccta
     4081 atattcctag aataacacaa tgttttaata ctttcttttt catgattgtt tttccttttc
     4141 gtttttttaa aattgcttgt tatattaaat aatcttagta attttattat ataacatttt
     4201 attatataac aatgattaac gtcataaaaa gtagttttga attcataatc gtttaaataa
     4261 aattataaga tcagttattt aaacagctag tagacataat gtgtatacta ccaagtaacg
     4321 cctgtaccaa gggatccagg cgctgatttt gtgaattttt attggtctag gttaaaagct
     4381 ctttttaagg cttggttttc tgcggttgtt tctttctaat tctttgtgaa tattgatcgg
     4441 tcatttttta aatacttgtt atttttgttt gtgtttgtta tgctttttac atatctcata
     4501 ttcatttatt attcttttct aaggttaata ataggataat ctctttcaga aagactgctc
     4561 gtacgagcag tcttttctta tttatcaagt ttttctacaa atacttcctc attttgactg
     4621 ttgtagtaat taggattgag ttttagttcc ccttttttat ttcctttgtt ggccactgat
     4681 aataacattg ggctaaagct taatattcct agtaggatag aaccgaatag caagtgtttt
     4741 tttgtcataa gtatttcctt cttcatttcc atagctgcca ccattttttc caattctttg
     4801 tttgttcgtc cattgtttct gttgcttcag aaagagtcgt tgaactattc tctgaagact
     4861 cttccgtata agtcaactgc agttgctcct gaagtttttc gatttgttgg ttggcttgca
     4921 gggtgagttg ttgttgctgg tcgagtagtt tttgtatatg agaattattt tgctctttga
     4981 gggcaagttg ttctcgtaaa aattcatttt cttcacgcaa ctgatctagc aacttgtttg
     5041 caaccacgtt tttgtttggt atataagcat ttttatcgtt gttttgttca tgtttatgca
     5101 acttatctag caacctttga ttgtctaagt tgcgagcaag ttgcaatact ccagaacgca
     5161 atacaacctt ataaccacgc tcatttttag ctatcatgga agaatctaat ttttggatat
     5221 gtttcgatat tgcttgccta ctcatcttaa aaagatctga caattccttt attgtaaagg
     5281 tttcttgtgg ttgcatactg gttgcctcct aggttgctac tggaataaat tatctaaatc
     5341 agataataaa ctttttttct ctttttccga aagtgcggac tctgtttgtt tttcaagagc
     5401 ttttttctct ttttctagtt gtttgatttg ttggaggagt tcaagttctc gtttcttctg
     5461 tttgattttt tcgttaattg ttgtagcggg ttcttcgtat tctttttgtt tatcagctaa
     5521 agccaatgct ttattttggt cttctttggt tggtgcatgt ttgttaaagt cgtctgcttt
     5581 tttgatctca ggtttgaacg taaattgata gccaataaca ggttttcccc gtccttttcc
     5641 gtatttcttt ttgattgtta gtcctttgaa gattgcagtt agttcttgac gtataggttt
     5701 tataactcgt ttatctatat caggttgctt ataactttta ggaatatcta ataattcaag
     5761 aaagtcttct ttggagaaat atgcgaaacc tttggttctg tattgcttta acagacgaaa
     5821 catagtcttt gcatagccac tttgtagctc gttgaattgt tgtaaagaat agcgtaccca
     5881 ctcgtctaaa ttatttaaca aaggcagtgc tttttcgtaa attcgtatat ctacgtaagg
     5941 attttctacg tccccgttga ttttaaattc tgtaaacatt acaaacatat cgtaagaaag
     6001 cccagactca cttctacgac caaagcgtaa agataagatt ttttcataag ttgcttttaa
     6061 atcttctaca aatcgtacat ttgcagttgc tttgtagtta cttaaatctt ttagttggtc
     6121 aaacgtaaaa cgaactgtct tatccccttt ttcacgcatt ctagacacga tgg
//
LOCUS       DASHHB010000099         6065 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.103, whole genome shotgun sequence.
ACCESSION   DASHHB010000099 DASHHB010000000
VERSION     DASHHB010000099.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6065)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6065)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6065
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.103"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            832..1566
                     /locus_tag="U2K71_002666"
     CDS             832..1566
                     /locus_tag="U2K71_002666"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM7316678.1"
                     /translation="MQNTFQSKSLYYQLVEILKQRIENSMVAHDKLPSERELTSFYGV
                     SRTTVRLALQELEKMGYIYRQHGKGTFVSELRTNATDLAGAYSFTEQMRAMGKHPETK
                     ILAFKKIEATKYLASHLNVQLGEPLFQLDRLRLADDLPMMIEKSYLPVKLFMSLSQEI
                     LFLKPLYNLFSENFNQIVRLADEEFYASIVQKEDSELLNIPEGSPVLHLVRSTYNLEN
                     EVIEFTLSVARADKFHYKIRHLRSTN"
     gene            1582..2751
                     /locus_tag="U2K71_002667"
     CDS             1582..2751
                     /locus_tag="U2K71_002667"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM7316679.1"
                     /translation="MFMFSKEELRKLGAENTTREIRQQPELWHETFDRYQETVADLDR
                     LFTDAKQHAGERPIRVIFTGAGTSQYVGDTLIPFLTSQGNTNQFIFQSIGSTDIVATP
                     KEYLIPDETTILVSFARSGNSPESLAAVKMANQLVNKIYHIVITCAPDGLLATQMPTK
                     KENLVLLMPNKANDAGFAMTSSFSCMTLTALLIFDQASNQQKERYVHQLSRLGEEVIQ
                     RESEIQGYLASDFHRIIYLGSGSLGGLTREAQLKVLELTAGKIATVFDTSLGFRHGPK
                     SFVNEQTLVFLFVSNDSYTQKYDVDMLEEIKNDGIAQRVVGIGQKGSLFSGDSFELVA
                     DSLLPDGYLALADIMVAQTIALLCSVKVGNTPDTPSPSGTVNRVVKGVTIYPYEK"
     gene            2767..3762
                     /gene="lacD"
                     /locus_tag="U2K71_002668"
     CDS             2767..3762
                     /gene="lacD"
                     /locus_tag="U2K71_002668"
                     /EC_number="4.1.2.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose-bisphosphate aldolase"
                     /protein_id="HEM7316680.1"
                     /translation="MLEWSENKRQAMERLSTPEGIITALAIDQRGALKKMMSALGMEA
                     TKEKIEQFKTLISQELTPYASAILLDPEYGLPAAQKRAAASGLLLAYEKTGYDQTVPG
                     RLPDLLSDCSVLRLKELGADAVKFLLYYDYEEAEEINRQKQVFVERIGSECMAEGLPF
                     YLELLSYDAKNVDTKSLSYAKAKPRKVIEMMKEFSKTQYKVDVLKVEVPVNMSFVEGN
                     ATGEIAYTRQEAAAYFKEQSDATDLPYIFLSAGVSAALFMETLRFAKHAGARFNGVLC
                     GRATWKDAVAPFVEKGEAATLEWLTEQGTQNIRQLNEVIRETAIPWYEKVSESTC"
     gene            3841..4767
                     /locus_tag="U2K71_002669"
     CDS             3841..4767
                     /locus_tag="U2K71_002669"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HEM7316681.1"
                     /translation="MNKRLLDYSASDFLKAKPMELKQAILASEGRTIMAENVPSSCPY
                     MGKSVTNAELERAAGADLILFNALDLFNPQISGVPLTLKENPIIWIKKALGRAIGVNL
                     EPVDKEVLMSENRTEISSGRIVSPETLKKANELGFNFICLTGNPGTGVSNRTIEKAIT
                     LSKSIFNGLIIAGKMHGAGVDEPIMNLDIAKRFIKTGVDILLVPAPYTVPYFMESDLR
                     GIVDYIKDFNRNKSIEEKVLVLTANGTSQDSSDQETIKKIALASKACGADIQHIGDSL
                     NGICLPENIFALGQAIRGYRHQIIMLGKSNYR"
     gene            4786..6036
                     /locus_tag="U2K71_002670"
     CDS             4786..6036
                     /locus_tag="U2K71_002670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIC"
                     /protein_id="HEM7316682.1"
                     /translation="MIDKVQKIAAKISNQRHLLALRDSMAAVMPLIIIGSFFMLLANF
                     PIEGFTKWLSEIGVQSFLNKASDSTFGIIGLAVTYSLGHNLAKTYGVDSLSAGLLSLS
                     GYVLLTPLLDADGSIGFPTKYFGTSGLFVGIIVALVSTEIFRWFVLKNITIKMPASVP
                     PNVSRAFVAIIPGFFVVLLWFIVVVICYKLGIENVHALIADTLAKPLSLLTKTLPGII
                     LVILIQCFFWMFGIHGAQVTGPIIEPLLLQNSDVNRIAYQAGKELPNIITYEFLYNFV
                     FSGGAGCLFALAILTFFFSKSQENKTLGKLSIAPVSFQVAEPLLFGFPTILNIKMLIP
                     FVTAPVVTTLITYFSMSMGLVAKPVGATIPWTTPPIIAGFLASGGRISGAVIQVITIA
                     INVLIYYPFFKLDDNAKLKSEKND"
BASE COUNT         2025 a          997 c         1246 g         1797 t
ORIGIN      
        1 attggcgcag cgaatccttg ataatgagct cggatgtgct aacgctagcg aggcagtcta
       61 actttaactg ttagctcctg ccgaagatcg tcacatccga ggaggctcat tgtcaaggac
      121 aatcggaata gcatacggaa agaagttctg caccttataa aatttgtcaa aaaaactgtt
      181 gccattccaa agtttgaaaa atgctatttc gaggtagaag gatattatat aagcacggtt
      241 gactaaacga agtgataata aaaaaagtta ttcaagaata agaaaaatta tgatacgaat
      301 tcagataaac tggagaatac agagaccttt aaaagggata agaaacaaca taaattcctc
      361 ttaagagaga acgaaaggac aaagccagca acttgataca cgagctagta ttagaggaat
      421 ttattctcat agactccctt ttgaatacgt ttcaacttta attttcatgt tcataaatga
      481 tttgaaacca aagcaaatgc gttgcacttt tcctccgtct tccgaaaatc gttcaattca
      541 ctggtaagta gtcatttgat agtttgaggc aaattgttga ttgatgaagg gtaggagcta
      601 aaaaaagata ccagttgaag agagaaaaag aaaaaaatag agggtgttct tgaatgaaaa
      661 aagttggaaa attatttttc atttcctttt attcctatta aagtcagtga tgtcaatcat
      721 attaacaaga ctaaaaatga aaataaaaaa aagattgttc ttagtattga ctcataaaaa
      781 ataccactac tatacttaca ttgtggttat agccagttag gagagacatc gatgcaaaat
      841 acctttcaat caaaatcact ttattatcag ctggtagaaa tattgaaaca acgaatcgaa
      901 aacagtatgg tcgctcatga caagctgcca tcagaaagag agttgaccag tttttatggc
      961 gttagtcgca cgacggtgcg actagcattg caagaactgg aaaaaatggg ctacatttat
     1021 cgacaacatg gaaaaggaac ctttgtttcg gaattaagga caaatgcgac tgatctagct
     1081 ggcgcatata gttttaccga acaaatgcgg gcaatgggca aacatcctga aaccaagata
     1141 ttggccttca agaagattga agcaactaaa tatttagcga gtcatctgaa tgttcaacta
     1201 ggtgagcctt tatttcaatt agaccgattg cgcttagctg atgatttgcc aatgatgatc
     1261 gagaaatcct atttgccagt gaagctattt atgtcccttt ctcaagaaat tctatttctg
     1321 aaacctttat ataatttgtt ttcagagaat ttcaatcaaa tcgtacggtt ggctgacgag
     1381 gaattttatg ccagcattgt ccagaaagaa gatagtgagt tgctaaatat tccagaagga
     1441 tcaccagtgc tccatttagt tcgctcaacc tataatctcg agaacgaagt gattgaattt
     1501 acattaagtg tagcgcgggc tgataaattt cattataaga tcaggcatct gcgttccact
     1561 aactgaattg gaggaaagaa aatgtttatg ttctcaaaag aagaactacg aaagctagga
     1621 gcagaaaata ctacccgaga aattcgacag caaccggaac tgtggcatga gacgtttgac
     1681 cgttatcaag aaacagttgc agatttagat cgtttgttta cggatgcgaa acaacatgct
     1741 ggagagcgac caatccgagt catttttaca ggggccggaa cctcgcaata tgtgggagat
     1801 acactaattc cgtttcttac tagtcaagga aatacgaacc agtttatttt tcaaagtatt
     1861 ggttcgaccg atatcgttgc tacgccaaaa gaatacctga ttcctgatga gacaacgatc
     1921 ttggtctcat ttgcccgtag tggaaatagt ccagaaagtc tagccgcagt taagatggcc
     1981 aatcagctgg tcaacaagat ctatcatatc gtgatcacct gtgcaccaga cggattattg
     2041 gctacacaaa tgcctacaaa aaaagaaaat ctagttttac taatgccaaa caaagccaat
     2101 gacgctggtt ttgctatgac aagcagtttc agctgtatga cgctgactgc gctacttatt
     2161 tttgatcaag caagcaatca gcaaaaagaa agatatgttc atcaattaag ccgactagga
     2221 gaagaagtca tccaacgaga atcagagatc caagggtatc tggcatcaga ctttcatcgc
     2281 atcatttact taggttcggg aagcttaggc ggattgacta gagaagcgca attaaaagtt
     2341 ctcgagttaa ctgctggaaa aatcgcaacc gtatttgata catctttagg attcagacat
     2401 ggtcccaaat cttttgtcaa tgagcaaaca ttggttttcc tgtttgtcag caatgactca
     2461 tatacgcaga aatacgatgt agacatgctg gaagaaatca aaaatgacgg tattgcccaa
     2521 cgcgttgttg gaattggtca aaagggttca ttgtttagtg gtgattcctt tgaattagtt
     2581 gcagattcgt tactgccgga tggctatctt gctttagcag atatcatggt agcccaaacg
     2641 atcgcattac tttgttcggt gaaggttggt aatacaccag atacaccatc gccaagtgga
     2701 acagtcaatc gtgtggtcaa aggcgtaacg atttatccat atgaaaaata ggaaagagtg
     2761 tgaagcatgt tagaatggtc ggaaaataaa agacaagcaa tggaacgtct atcgacgcca
     2821 gaaggcatta ttaccgcttt ggcgatcgat cagcgaggcg cgctgaagaa aatgatgtcg
     2881 gctttaggga tggaagcgac aaaggaaaaa attgagcagt ttaaaacact aatttcccaa
     2941 gaattgactc cttatgcctc agcaatttta ttggatcctg aatacggact gcctgctgca
     3001 cagaagcgtg cagcagcgag cggtctgctg ttggcatatg aaaaaactgg ttatgatcaa
     3061 accgtacccg gccgcttgcc ggatctgctg tcggactgtt cagttctgcg attgaaagag
     3121 ttaggtgcgg atgcggtgaa atttttactg tattacgatt atgaagaagc agaggaaatc
     3181 aatcggcaaa agcaagtttt tgttgaacga attggaagcg aatgcatggc tgaaggactg
     3241 ccattttatt tagagttatt gtcatatgat gctaaaaatg tagatacgaa atcgctttcc
     3301 tatgccaaag caaaaccgcg caaagtgatc gaaatgatga aagaattctc gaaaacccag
     3361 tataaagtag atgtcttaaa ggttgaagta ccagtaaaca tgtcatttgt tgaaggaaat
     3421 gcaacagggg agatcgccta tacacgtcaa gaagctgccg cttattttaa agaacaaagc
     3481 gatgcaacag atttgccgta tatcttctta agtgctgggg tatcagcggc attattcatg
     3541 gagacattgc gttttgccaa acatgctggg gcacgattca acggtgtact ctgtggcaga
     3601 gcaacttgga aagatgcagt cgctcctttc gttgaaaaag gagaagcggc aactttagaa
     3661 tggctgacag aacaagggac acaaaacatt cgtcaattga atgaagtgat aagagaaact
     3721 gccatcccat ggtatgagaa agtaagtgaa agcacttgtt aatgtttcta atatattagt
     3781 agtgataaga gatatgtgtg attataattt atcattattg aaactgggag gatattttat
     3841 atgaataaaa gattgttaga ttattcagct tcagattttt tgaaagctaa gccgatggaa
     3901 ttaaagcagg caattttggc ttctgaaggt agaacaatta tggcagaaaa tgtgccaagt
     3961 agctgtcctt acatgggaaa atcagtgaca aatgcagaat tggagagagc tgctggagct
     4021 gatttaattt tgtttaatgc actagatctt tttaatcctc aaatttcagg tgtaccttta
     4081 actctaaaag agaatccaat aatctggatt aaaaaagctt taggaagagc tattggtgta
     4141 aatttagaac ctgtagacaa agaagttttg atgagtgaaa atcgaactga aatttcaagt
     4201 ggtagaatcg tttctcctga gactttaaaa aaggcaaatg aattaggctt taattttatt
     4261 tgtttaactg gaaatccagg aacaggagta tctaatagaa ctattgagaa agcaataaca
     4321 ttatccaaat caatttttaa tggattaatt atcgctggaa aaatgcatgg tgctggtgtt
     4381 gatgaaccaa ttatgaactt agatatagca aaaagattta ttaaaacagg tgtagatatt
     4441 ttattagttc ctgctccata cactgttccg tatttcatgg aaagtgattt gcgaggaatc
     4501 gttgattata taaaagattt taatagaaat aaatctattg aggaaaaagt tctagtacta
     4561 acggctaatg gaacaagcca agactcttct gaccaagaaa ctattaaaaa aattgcatta
     4621 gcatcaaaag catgtggagc tgatattcaa cacatagggg attctttaaa tggtatttgt
     4681 cttccagaaa atatttttgc tttgggtcaa gcaattcggg gatatcgtca tcaaattatt
     4741 atgttaggaa aatctaatta tcgttaatta aggaggaaat gaaagatgat cgacaaagtt
     4801 caaaaaatag ctgcaaaaat tagtaatcaa agacatctac tagctttaag agacagcatg
     4861 gctgcagtta tgccattaat cattattggt tcctttttta tgttattagc aaatttcccg
     4921 atagaaggat ttacaaaatg gttatctgaa attggagtac aatccttttt gaacaaagca
     4981 tcagattcaa catttggtat tattggatta gctgttacct actctttggg acacaatcta
     5041 gcaaagactt atggtgtaga ctcattatca gcaggattat tatctttgag tggatatgtt
     5101 ttacttacac ctttattgga tgcagatggt tctataggat ttccaacaaa atactttggt
     5161 acctcgggtc tttttgttgg tattattgta gcacttgtat ctacagaaat tttccggtgg
     5221 tttgtactta aaaatattac aattaaaatg ccggcaagcg ttccaccaaa tgtaagccga
     5281 gctttcgttg ctattattcc tggctttttt gtcgttctac tatggtttat tgttgttgta
     5341 atttgttata aacttggaat tgaaaatgtt catgctctta ttgcagatac actcgcaaaa
     5401 ccactatctt tactgacgaa aactttacca ggaattatat tggttatact tattcaatgt
     5461 ttcttttgga tgtttggtat tcatggtgca caggtaacgg ggccaataat tgaaccgtta
     5521 ttattgcaaa actctgatgt aaatagaatt gcttatcaag ctggaaaaga acttcctaac
     5581 attataactt atgaattctt atataacttt gttttttctg gtggtgcagg ttgtttattt
     5641 gcacttgcca ttttaacatt tttcttttca aagagtcagg aaaataaaac tttaggtaag
     5701 ttatcaatcg ctccagtaag ttttcaagta gcagagcccc ttctatttgg gtttccaact
     5761 atattaaata taaaaatgtt aattccattt gtaactgcac cagtagtaac aacattaatt
     5821 acttattttt caatgagtat gggattagtt gcaaaacctg taggagcaac aatcccatgg
     5881 acaactccac cgataattgc cggattttta gcttctggtg gacgaataag tggtgcagtg
     5941 attcaagtaa taacaattgc aataaatgtt ttgatatact atccattctt taaattagat
     6001 gataatgcca agctaaaaag cgaaaaaaat gattagaact acctatatgt ttctaactgt
     6061 agtta
//
LOCUS       DASHHB010000100         6028 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.104, whole genome shotgun sequence.
ACCESSION   DASHHB010000100 DASHHB010000000
VERSION     DASHHB010000100.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 6028)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6028)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6028
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.104"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(68..370)
                     /locus_tag="U2K71_002671"
     CDS             complement(68..370)
                     /locus_tag="U2K71_002671"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316683.1"
                     /translation="MLMEGKTQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINL
                     YYSNKNYFKIPATKTRMKKDVYFLFDIVTDVPDARSDHRRYDYLKYTFVDPERYKD"
     gene            complement(590..956)
                     /gene="ssrA"
                     /locus_tag="U2K71_002672"
     tmRNA           complement(590..956)
                     /gene="ssrA"
                     /locus_tag="U2K71_002672"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
     gene            complement(1127..1900)
                     /locus_tag="U2K71_002673"
     CDS             complement(1127..1900)
                     /locus_tag="U2K71_002673"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346978.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Cof-type HAD-IIB family hydrolase"
                     /protein_id="HEM7316684.1"
                     /translation="MSNYRAITFFDLDGTLLDAKSKITPEVAKAMNALKENNVLPVIA
                     TGRTEAEIHSIMEDAGITSAVTMNGSFIRVDGEEIYSQTFSSEECQKMYDHVQLNGHE
                     LSYYNDQKIWCTGHSQTMINAYDFIHSDVPEIDPLGFKENTVNMLLILSQSGDEYYHE
                     HFPELTFYRNGPYSIDTVKKGISKGAGVKRLQKELQLEKVPTFGFGDGPNDFALLEAC
                     DNKIAMGNAYDGLKELSTFITKKNTEGGIVHALKYFDLI"
     gene            2044..2394
                     /locus_tag="U2K71_002674"
     CDS             2044..2394
                     /locus_tag="U2K71_002674"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEM7316685.1"
                     /translation="MIFFSIGILFLIIGSLFRILPSKGNLPFYGYHSPLAAKTDAHWR
                     LAQKTSGNWFFLMGLLMALIGYYLKTSGHTNYFLIEMLLLVFPIMPIFIMTEKKLQKY
                     DLETGGNDNEYFND"
     gene            2375..3091
                     /locus_tag="U2K71_002675"
     CDS             2375..3091
                     /locus_tag="U2K71_002675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7316686.1"
                     /translation="MNILMIEDNQSVSEMMQMFFLNEGWEATFKYDGKEGLDAFLENP
                     QHWDMITLDLNLPTLDGMLVGREIRKVSSTVPIIMLTARDSESDQVIGLEMGADDYVT
                     KPFSPLTLIARMKALHRRSELAEAREEAVVSDDHFDIETSHFKMNTKTREAYLDNRPI
                     EGLTPKEFDLLFTLAKKPRQVFSREQLLELVWDYQYFGDERTVDAHIKKLRQKIEKVG
                     PQVIQTVWGVGYKFDDSGVA"
     gene            3091..4539
                     /locus_tag="U2K71_002676"
     CDS             3091..4539
                     /locus_tag="U2K71_002676"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM7316687.1"
                     /translation="MRYLYQQLLAFCGMVALILLIVGVSFTQLTKQTIEENNYQQLFG
                     YAESVEKTTQTYADTLPQFSQDQAFQNALLLTEQVLTEQNVNFIFIDKNERVIYPTNT
                     ALLNFTLTADQWKNLRNGRQEKFTSNKNILGQAQATSYALVPFNLNHEFYGALVVSQP
                     ARNIDNSVRPITLNLFKGFIFSSIVAVIASYAFAAFQVKRINRLRNATKEVTNGNFDV
                     QLPVHDKDEFDELADDFNKMTNSLKESQAEIEEQENRRRQFMADASHEMRTPLTTING
                     LLEGLEYNAIPENQRENAIKLMKNETERLIRLVNENLDYEKIRTNQISMVIKKFNGTE
                     TLKNIVAQLEAKAEAAGDTLTLKAVDDIDVYADYDRFVQIMVNIIQNAIQFTENGQIT
                     ITLEKGYLETIITIEDTGIGMSEQQMKSIWDRYYKVDPSRKNTKYGESGLGLPIVQQL
                     VRLHKGKLEVESELGKGTKFAVRLPDVEIKKE"
     gene            complement(4610..5119)
                     /locus_tag="U2K71_002677"
     CDS             complement(4610..5119)
                     /locus_tag="U2K71_002677"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316688.1"
                     /translation="MKPEYLIHAEHLTVILCPLEKQHKILEHLKKEVLVAKLDNFAII
                     QKNWPMFPYLNLKDHVLLDVPEKEIKQDRLAYQEKLDISPSLLNCAAAELTSFEKVKL
                     QLLHALLSKRNNIVIEDTFDELSVLEIQELLHLLSYLAHEENQGILLFTHDATIAQSP
                     YIDRLEPAG"
     gene            complement(5109..5834)
                     /locus_tag="U2K71_002678"
     CDS             complement(5109..5834)
                     /locus_tag="U2K71_002678"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316689.1"
                     /translation="MKIIRYATARLIYYKKQTILYCLFSSFSAFLLIACLTLYHLQSA
                     LANQVQDRLSFIGASASTELLPALELASRFYIKGIFSLFFLFAFLFIFFFYYSVRQNK
                     QELINWHLTGLSKTKLFLFIFWQLFFPLFLCCILVLLWIVSFQSVYEALLQRINFLVL
                     KTTELPDIRDILTSVSGLTIPMDQQTFFTIDFQNDLFFTDILKGFAQTLIGLSGCCLI
                     LSLFPFTFFIHHLQSRRPGRNET"
BASE COUNT         2052 a         1050 c         1170 g         1756 t
ORIGIN      
        1 aaaaatatcc cttttataag tatggtgata aaatccgttc cgggctacct tttttaggta
       61 gcctacttta atctttatac ctttctggat caacgaaagt atacttcaaa tagtcataac
      121 gccgatgatc gctccgtgcg tctggcacat cagtcacgat atcaaacaaa aaatacacat
      181 ctttcttcat tctggttttt gttgccggaa ttttaaagta gttcttatta gagtaataga
      241 gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc cacaccttat
      301 aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgt gtttttcctt
      361 ccatcaacat aatgttcacc tcaaaaagag tatacgaact aatgttcttt ttgtaaagta
      421 ggaatatcat tgataatatg gatggaacat atatattaaa aagtgttctt tttgaatgct
      481 attttgaatg ccattttcat tcatttaacg aaaatctgcg aaaataaaaa atgctcaaac
      541 ccttataaaa taaggattta agcatctagc gaaatctgct gaaatctcac aatggagacg
      601 gcgggagtcg aacccgcgtc caaacacatc gccacttaaa aatctacgtt catagtcaac
      661 tcatttaagg attcacttta cagcttgccg agtgacaggc attctgtatc gctagtctga
      721 tgttctcttt ttcaacttac agacggaaag ttgaaacgta tcccactaaa gttaggaccc
      781 ttactcgagc acatgggcga tgccgagagg atctacgcta actgttttta ggcagctaaa
      841 gcgtaagagt tgttttcgtt tttagcagtt atatttaact gtaacgtttt tacgtagacg
      901 taacctacga aacgcaattc aagctcgact gtgcctgtcg aatccgtaac gtccccgtta
      961 gctaacaagg gttacgtgta tttttcatac aaaattagta tagcatatat tgggataaat
     1021 tcaataaaaa agcgaatcat atcccattat ttatataata actgcccctt tcttaagaaa
     1081 aaataagcga ttttcttaag aaaagaggta gcttcaaaaa aagatcttaa atcagatcaa
     1141 aatacttcaa tgcgtggaca attccgcctt ctgtattttt ctttgtgata aaggtagaaa
     1201 gttcttttaa tccgtcatag gcgttgccca tagcgatttt gttgtcgcaa gcttccaaca
     1261 aggcaaaatc attcggacca tcgccaaatc caaaagtagg aactttttcc agctgaagtt
     1321 ctttttgcag acgtttgaca cctgcccctt ttgagattcc tttcttcact gtatcgatcg
     1381 agtaaggacc attccgataa aaagttagct caggaaaatg ttcgtgatag tattcgtcac
     1441 ctgattgaga taagatcagc aacatattta ctgtgttttc tttaaagcct aaaggatcaa
     1501 tttccggaac atctgaatga atgaagtcgt aagcattgat cattgtttgg ctatgaccgg
     1561 tacaccagat tttctgatcg ttatagtaag aaagttcatg accgttcaac tgtacatgat
     1621 catacatttt ttgacattct tctgaagaaa atgtttgaga atagatttct tcgccgtcta
     1681 ctcgaatgaa agaaccattc atggtgaccg cagaagtaat gcccgcatct tccatgatag
     1741 agtggatttc tgcttctgta cgtcctgtag cgatgacggg gagaacattg ttttctttta
     1801 atgcgttcat agcttttgcc acttcgggtg tgatttttga tttcgcatct agtaatgttc
     1861 catcaagatc aaagaaagtg attgcacgat aatttgacac aaataaaact ccttttttat
     1921 tggaataagt ttatcttaac aaagaatgaa caaaagacaa acctttcgtg aagaattcct
     1981 gaacatattg gttcagtcaa caatttagag tatactataa gaagagaatt taggagggaa
     2041 agaatgattt ttttcagtat tggtatctta ttcttaataa ttggcagtct ttttcggatt
     2101 ctgccctcaa aaggaaatct gcctttttat gggtatcatt cgcctcttgc cgcaaaaaca
     2161 gacgctcatt ggcggctggc tcaaaagaca agcggaaact ggttttttct tatgggctta
     2221 ctgatggcat tgatcggcta ttatttaaag acatctgggc atacgaatta ttttctgatt
     2281 gaaatgcttc tgctagtatt tccaattatg cctattttta ttatgacaga aaagaaatta
     2341 cagaaatatg acctggaaac tggagggaac gataatgaat attttaatga ttgaagataa
     2401 tcaatctgtt tctgaaatga tgcagatgtt tttcttaaat gaaggttggg aagcaacttt
     2461 caagtatgat ggaaaagaag ggctcgatgc ctttttagaa aacccacaac attgggatat
     2521 gattacacta gacctgaatt tgcccacact ggacggtatg ctagtaggaa gagagatccg
     2581 caaagtttca agtactgtgc cgattatcat gctgacagct agagattctg aaagcgatca
     2641 ggtgatcggc ttagagatgg gggcagatga ttatgtcaca aaacctttca gtcctctgac
     2701 gcttattgct cgaatgaaag ctttgcatcg ccgtagtgag ttggcagaag caagagaaga
     2761 agcagtagtt tcagatgatc attttgatat cgagactagt catttcaaga tgaatacgaa
     2821 aacacgagaa gcgtatctgg acaaccggcc aattgaagga ctgacgccta aagagttcga
     2881 tctattgttt actttagcaa aaaaaccacg tcaagtattc tctcgggagc agttgcttga
     2941 attagtctgg gattatcagt actttggtga tgaacggaca gtggacgccc atatcaagaa
     3001 attacgacaa aaaatcgaaa aggtcggacc acaagtcatc caaactgttt ggggagtcgg
     3061 ctacaaattc gatgattctg gtgttgccta atgcgttatt tgtatcagca actcttagct
     3121 ttctgcggaa tggtcgcgtt gatcttattg atcgttggag tttcttttac acaactgaca
     3181 aaacaaacga ttgaagaaaa caattaccag caactgtttg gatatgctga atcagttgaa
     3241 aagacaacgc aaacctatgc cgatacgctc ccgcagttta gtcaagatca ggcgtttcaa
     3301 aatgcattgc tgctgacaga gcaagtactg acagaacaaa atgtgaactt tatatttatc
     3361 gataaaaacg aacgtgtcat ttatccgact aatacagctt tactgaattt taccttaaca
     3421 gctgatcaat ggaaaaatct gaggaatgga cgtcaagaaa aattcacatc gaataaaaat
     3481 attttaggac aagcacaggc gacctcttac gcacttgtgc catttaattt gaatcatgaa
     3541 ttctatggag cacttgtagt tagtcaacct gcgcgaaata tcgataacag cgttcgtcca
     3601 attacactga acctgttcaa aggatttatt ttttccagta tcgttgcagt tattgccagc
     3661 tatgcttttg cagcattcca agttaagcgt atcaatcggt taaggaatgc gacgaaagaa
     3721 gtgacgaatg gtaattttga tgtacagctc cccgtacatg acaaagatga atttgatgag
     3781 ttagcagatg atttcaataa aatgacgaat tcattgaaag aatctcaagc agaaattgaa
     3841 gaacaagaaa atcgtcgaag acaatttatg gcagatgctt ctcacgagat gagaacacct
     3901 cttacgacaa tcaacggact tctggaaggg ttagaataca atgcaatccc tgaaaatcag
     3961 cgagaaaacg cgattaaatt gatgaaaaat gaaacagagc gtctgatccg tttggtgaat
     4021 gaaaacttgg attatgaaaa aatccgtacg aatcaaatca gtatggtcat taaaaaattc
     4081 aatggaacag aaacattgaa aaatattgtt gcacagttag aagctaaagc agaagcagca
     4141 ggagatacat tgacattgaa agccgttgat gatattgatg tttacgctga ttatgaccga
     4201 tttgtccaaa tcatggtcaa tattattcaa aacgcgatac agtttactga aaacggccaa
     4261 atcacgatta ctttagaaaa aggctatctt gaaacgatca tcacgattga agatacggga
     4321 attggaatgt ctgaacaaca aatgaaaagt atctgggatc gttattataa agtagatcct
     4381 tcaagaaaaa atacaaaata tggtgaatca ggattaggtt taccaatcgt gcagcaactt
     4441 gtacgcttgc ataaaggaaa attagaagta gaaagcgaac taggaaaagg aacaaaattc
     4501 gctgttcgtt tgcctgacgt agaaatcaaa aaagagtaaa aaacaagcgg tataaaagat
     4561 catgaatcga tcttctatac cgcttgtttt ttactataaa atgattgatt tagcccgcgg
     4621 gctctaaacg atcaatataa ggactttgag caatagtagc atcatgagta aagagtaaga
     4681 taccttgatt ttcttcatga gctaaataag aaagcaagtg gagtagctct tggatctcca
     4741 atacactaag ttcatcaaat gtatcttcaa taacaatatt gtttcgcttg gaaagcaagg
     4801 catgaagtaa ttgcagcttt actttttcaa aagacgtcaa ctcagctgcc gcacaattaa
     4861 gcaaggaagg agagatatca agtttttctt gataggccaa acgatcttgt ttgatttctt
     4921 tttccggtac atccaacagg acatggtctt taagattcag ataaggaaac atcggccaat
     4981 ttttttggat gatcgcaaaa ttatccagtt ttgcaacaag aacctctttt ttcaagtgct
     5041 ccaatatttt atgctgtttt tcgagaggac acaaaataac ggtcaagtgt tcagcatgaa
     5101 tcaaatattc aggtttcatt acgtccaggc ctcctacttt gcaaatgatg gataaaaaaa
     5161 gtgaaaggga atagactaag aatgagacag catccactga gcccaataag agtttgggca
     5221 aatcccttaa gtatatctgt gaaaaaaaga tcattttgga aatcgatggt aaaaaaagtc
     5281 tgttgatcca tcggaattgt aagtccgctt accgaagtca agatatcacg gatatctggc
     5341 agttcagtag tctttaagac aagaaaattg atcctttgaa gaagcgcttc ataaactgac
     5401 tggaagctta cgatccacaa taaaaccaag atacaacaaa gaaagagagg aaagaacagc
     5461 tgccagaaaa taaaaaggaa taatttggtt ttagaaagac cagtcaggtg ccaattgatc
     5521 aattcctgct tgttttgcct gactgaatag taaaagaaaa agataaaaag gaaggcaaat
     5581 agaaaaaata aactgaatat gccttttata taaaatcggc tagctaattc taaagccgga
     5641 agaagctctg tagatgcaga agcgccaata aatgaaagcc gatcttggac ttgattggct
     5701 aaagccgatt gcaaatggta aagagtcagg catgctatca ataaaaaagc agaaaagctt
     5761 gaaaataaac aatagagtat tgtctgcttt ttgtaataaa ttaatcgagc agtagcatag
     5821 cggattattt tcatactttt cacctctata aaaagtatag aaaatgaata tgaataaagt
     5881 atgaaattcc tttagtgaaa tcacgaatta aaaccagaaa atattttgta aaaagcaaga
     5941 ggttgtgaag aagctgccct attctattac cgtagatgca agcttttgaa cactgtttat
     6001 tcgaaaagag gagcagcgac aataattt
//
LOCUS       DASHHB010000101         5838 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.105, whole genome shotgun sequence.
ACCESSION   DASHHB010000101 DASHHB010000000
VERSION     DASHHB010000101.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5838)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5838)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5838
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.105"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(107..322)
                     /locus_tag="U2K71_002679"
     CDS             complement(107..322)
                     /locus_tag="U2K71_002679"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316690.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQGSVAKF"
     gene            complement(865..3015)
                     /locus_tag="U2K71_002680"
                     /pseudo
     CDS             complement(865..3015)
                     /locus_tag="U2K71_002680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3161..3736
                     /locus_tag="U2K71_002681"
     CDS             3161..3736
                     /locus_tag="U2K71_002681"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7316691.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3950..4645
                     /gene="vanR"
                     /locus_tag="U2K71_002682"
     CDS             3950..4645
                     /gene="vanR"
                     /locus_tag="U2K71_002682"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEM7316692.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4623..5777
                     /locus_tag="U2K71_002683"
     CDS             4623..5777
                     /locus_tag="U2K71_002683"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEM7316693.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1822 a         1126 c         1096 g         1794 t
ORIGIN      
        1 acggctacaa taatcacatc ctgctgaaat tgctttcctt taaaatgatt catcgtcatt
       61 cctcctgcta tctttttcta ttattctacc ttatttgata gtagatttaa aactttgcaa
      121 cagaaccctg ttcaaaatct aaaatcaaca catcatgggg agtgtgataa taaatatttt
      181 cccttaatgt accaacaatg gtaattcggt caattgacca ttgcattttt aagggggtgc
      241 acatagtttg tgcatttaac cgcctgttag aaatacggtt aagtttttcc cactcattta
      301 cgattatgcc ttttttactc aaaaatagct cctcctcaac gaaaaaagag attcttggaa
      361 agaatttcca gagttcacgt aactctaaaa atccacatcc aaaaatctct agcttgaaga
      421 aacctaaaaa tattcgtata atgaacttga gttacggaac taggtgaagt agtttccgta
      481 aaggtcgaga gtgcgtcaac actgctcgac tattttttta ctcttttttc tcattttctt
      541 tgattatagc actcctctag aaaaaataaa aagacctcag ggaatttcat tccccgaacc
      601 ccttgagcct cctcctgccg aaatagaatg gcgctgccag catttatttc gttcagctat
      661 cggctacaca atctaacacg accgttccgc taccagtcgc cttacaggct ccattttcgc
      721 tcctctaacg tgtttcaccg attgtgccga taactaaacg aaataaacgc taaaaccgct
      781 cagaacgcaa aatatgccat tttagagcta ttgtagaaaa tatagtttga aatgcaattt
      841 tcttctttca gctttttatt tcccacttgt aaatcggtag atggtggctc cttttccagt
      901 tccataatgt ggatttgcat ctgcatgtag tgatgaaaca cctagctgca ttctcatacc
      961 atctgacgaa gatgttgtac cgtcgcccca atagaaaggc aattgtaatt tatgatgaaa
     1021 gtttactaat atggcttggg ctttattcat ggcatcttca tacatgcgcc attgagatac
     1081 attggctagt tgcttatatg taagtccggg tgtggcttcg gccatcttgc tcaagccaat
     1141 attcattccc attcctaaaa gggcagccat gataatgatt gtttcttcct tatctggttt
     1201 tcgattattg gaagcatgag tgaattgctc atgaaatcct gttatatggg ccacatccat
     1261 gagtaaatca gttaatttta ttcttggtag catctgataa aggcttgcac taaatttttt
     1321 tgcttcttct ggaacatctt tttctaagcg tgcaagtgat agctttcctt tttcaagaga
     1381 aaccccatct aacttattgg aattggcagc taaccacttt aacctttcat taaagctgct
     1441 ggttctctcc gttatataat cttcgaatga taaactaact gataatctcg tattcccctt
     1501 cgattgattc catgtatctt ccgaaaacaa atattcctca aaatccctat attgtctgct
     1561 gccaacaatg gaaacatctc ctgcccgaac atgctcccga agttctgtta aaacagccat
     1621 ttcatagtaa tgacgattaa ttgttgtacc atcatcctcg tataaatgtc ttttccatcg
     1681 ttttgaaata aaatccacag gtgagtcatc aggcactttt cgctttccag attcgttcat
     1741 tcctcggata atctcaacag cttgtaaaag tggctcattt gcctttgtag aatgaaattc
     1801 caatactctt aatagcgttg gcgtatattt tcttagtgaa taaaaccgtt tttgcagtaa
     1861 gtctaaataa tcatagtcgg caggacgtgc aagttcctga gcctcttcta ctgaagagac
     1921 aaaggtattc cattcaataa ccgattctaa aaccttaaaa acgtctaatt tttcctctct
     1981 tgctttaatt aatgcttgtc cgatgttcgt aaagtgtata actttctcat ttagcttttt
     2041 accgttttgt ttctggattt cctcttgagc cttacgacct tttgataaca aactaagtat
     2101 ttgcctatca tgaatttcaa acgctttatc cgttagctcc tgagtaagtt gtaataaata
     2161 gatggttaat atcgaataac gtttattttc ttgaaagtca cggaatgcat acggctcgta
     2221 tcttgagcct aagcgagaca gctgcaacag gcggttacgg tgcaaatgac taatttgcac
     2281 tgtttctaaa tccattcctc gtatgtattc gagtcgttct attattttta gaaaagtttc
     2341 gggtgaagga tgacccggtg gctcttttaa ccaacccaat atcgttttat tggattcgga
     2401 tggatgctgc gaggtaataa tcccttcaag cttttctttt tgctcatttg ttagagattt
     2461 actaaccgta ttaaatagct tcttttcagc cattgccctt gcttcccaca ccattctttc
     2521 aagtgtagtg atagcaggca gtataatttt gttttttctt agaaaatcta tgcattcatg
     2581 cagtagatga atggcatcac cattttccaa agctaattga tgaaggtact taaatgtcat
     2641 tcgatattca ctcagggtaa aagttacaaa gtcgtattca cttcgaattt ctttcaaatg
     2701 atcccaaagt gtattttccc tttgaggata atgatcaagc gaggatggac taacaccaat
     2761 ctgtttcgat atatattgta tgaccgaatc tgggatgctt ttgatatgag tgtatggcca
     2821 accgggatac cgaagaacag ctaattgaac agcaaatcct aaacggtttt cttccctcct
     2881 tcgcttatta actatttcta aatcccgttt ggaaaaagtg aagtaggtcc ccagtatcca
     2941 ttcatcttca gggatttgca taaaagcctg tctctgttcc ggtgtaagca attctctacc
     3001 tctcgcaatt ttcattcagt atcattccat ttctgtattt tcaatttatt agttcaatta
     3061 tatatcaata gagtgtactc tattgataca aatgtagtag actgataaaa tcatagttaa
     3121 gagcgtctca taagacttgt ctcaaaaatg aggtgatatt ttgcggaaaa tcggttatat
     3181 tcgtgtcagt tcgactaacc agaatccttc aagacaattt cagcagttga acgagatcgg
     3241 aatggatatt atatatgaag agaaagtttc aggagcaaca aaggatcgcg agcaacttca
     3301 aaaagtgtta gacgatttac aggaagatga catcatttat gttacagact taactcgaat
     3361 cactcgtagt acacaagatc tatttgaatt aatcgataac atacgagata aaaaggcaag
     3421 tttaaaatca ctaaaagata catggcttga tttatcagaa gataatccat acagccaatt
     3481 cttaattact gtaatggctg gtgttaacca attagagcga gatcttattc ggatgagaca
     3541 acgtgaaggg attgaattgg ctaagaaaga aggaaagttt aaaggtcgat taaagaagta
     3601 tcataaaaat cacgcaggaa tgaattatgc ggtaaagcta tataaagaag gaaatatgac
     3661 tgtaaatcaa atttgtgaaa ttactaatgt atctagggct tcattataca ggaaattatc
     3721 agaagtgaat aattagccat tctgtattcc gctaatgggc aatattttta aagaagaaaa
     3781 ggaaactata aaatattaac agcctcctag cgatgccgaa aagccctttg ataaaaaaag
     3841 aatcatcatc ttaagaaatt cttagtcatt tattatgtaa atgcttataa attcggccct
     3901 ataatctgat aaattattaa gggcaaactt atgtgaaagg gtgataacta tgagcgataa
     3961 aatacttatt gtggatgatg aacatgaaat tgccgatttg gttgaattat acttaaaaaa
     4021 cgagaattat acggttttca aatactatac cgccaaagaa gcattggaat gtatagacaa
     4081 gtctgagatt gaccttgcca tattggacat catgcttccc ggcacaagcg gccttactat
     4141 ctgtcaaaaa ataagggaca agcacaccta tccgattatc atgctgaccg ggaaagatac
     4201 agaggtagat aaaattacag ggttaacaat cggcgcggat gattatataa cgaagccctt
     4261 tcgcccactg gagttaattg ctcgggtaaa ggcccagttg cgccgataca aaaaattcag
     4321 tggagtaaag gagcagaacg aaaatgttat cgtccactcc ggccttgtca ttaatgttaa
     4381 cacccatgag tgttatctga acgagaagca gttatccctt actcccaccg agttttcaat
     4441 actgcgaatc ctctgtgaaa acaaggggaa tgtggttagc tccgagctgc tatttcatga
     4501 gatatggggc gacgaatatt tcagcaagag caacaacacc atcaccgtgc atatccggca
     4561 tttgcgcgaa aaaatgaacg acaccattga taatccgaaa tatataaaaa cggtatgggg
     4621 ggttggttat aaaattgaaa aataaaaaaa acgactattc caaactagaa cgaaaacttt
     4681 acatgtatat cgttgcaatt gttgtggtag caattgtatt cgtgttgtat attcgttcaa
     4741 tgatccgagg gaaacttggg gattggatct taagtatttt ggaaaacaaa tatgacttaa
     4801 atcacctgga cgcgatgaaa ttatatcaat attccatacg gaacaatata gatatcttta
     4861 tttatgtggc gattgtcatt agtattctta ttctatgtcg cgtcatgctt tcaaaattcg
     4921 caaaatactt tgacgagata aataccggca ttgatgtact tattcagaac gaagataaac
     4981 aaattgagct ttctgcggaa atggatgtta tggaacaaaa gctcaacaca ttaaaacgga
     5041 ctctggaaaa gcgagagcag gatgcaaagc tggccgaaca aagaaaaaat gacgttgtta
     5101 tgtacttggc gcacgatatt aaaacgcccc ttacatccat tatcggttat ttgagcctgc
     5161 ttgacgaggc tccagacatg ccggtagatc aaaaggcaaa gtatgtgcat atcacgttgg
     5221 acaaagcgta tcgactcgaa cagctaatcg acgagttttt tgagattaca cggtataacc
     5281 tacaaacgat aacgctaaca aaaacgcaca tagacctata ctatatgctg gtgcagatga
     5341 ccgatgaatt ttatcctcag ctttccgcac atggaaaaca ggcggttatt cacgcccccg
     5401 aggatctgac cgtgtccggc gaccctgata aactcgcgag agtctttaac aacattttga
     5461 aaaacgccgc tgcatacagt gaggataaca gcatcattga cattaccgcg ggcctctccg
     5521 gggatgtggt gtcaatcgaa ttcaagaaca ctggaagcat cccaaaagat aagctagctg
     5581 ccatatttga aaagttctat aggctggaca atgctcgttc ttccgatacg ggtggcgcgg
     5641 gacttggatt ggcgattgca aaagaaatta ttgttcagca tggagggcag atttacgcgg
     5701 aaagcaatga taactatacg acgtttaggg tagagcttcc agcgatgcca gacttggttg
     5761 ataaaaggag gtcctaagag atgtatataa ttttggctct ttgtcaaatg atgttggtaa
     5821 agagaaatct tagaataa
//
LOCUS       DASHHB010000102         5463 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.107, whole genome shotgun sequence.
ACCESSION   DASHHB010000102 DASHHB010000000
VERSION     DASHHB010000102.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5463)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5463)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5463
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.107"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..921
                     /locus_tag="U2K71_002684"
     CDS             <1..921
                     /locus_tag="U2K71_002684"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316694.1"
                     /translation="DDPYWLGKAPLGENIANKSVTFPIKSSAYSLYQANMEEEFVLGQ
                     TYTITMKATKPPIQTFIVYNEDSGDYRYGNLEPVEGLVDTWGLTFTPQKVGVNYPKRL
                     TIIQYPQSTTGACQIDWLKIEKGDTRTPNISQFKYFGEGLKDSNNPNDYSWDVTPEYT
                     EKGLNDAVNVYDPQRVEGLKNFADGIQIAGDKVISENDCTVYTLTKDNSQSFIDGYAT
                     FIKHGKEVIVNGTVKFKKAYAFGVPLDDEVPDEFVAKIVHGMLTGPSGTSSVSKAMYV
                     RKDLGTIITNSEFAANEWFTFHGNYWVGEE"
     gene            924..1370
                     /locus_tag="U2K71_002685"
     CDS             924..1370
                     /locus_tag="U2K71_002685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316695.1"
                     /translation="MKNIWKYGRTGGEYAGQVLDDMVMTVPFTDVPPLEGIRADGEPL
                     TIADQMFDPKLNQWIVLVNVLDHNDLNNLKAMYESLENENGDLKQLNAKLMLSDVAIK
                     QENTALKEKADGLAQINSKTMLASFQNSKDIAEIKEQLNPASKGGE"
     gene            1372..1509
                     /locus_tag="U2K71_002686"
     CDS             1372..1509
                     /locus_tag="U2K71_002686"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEM7316696.1"
                     /translation="MFSFSDVKMMYDWGCFTDDQVRLFVPLCITDEEAEKIINKDKSA
                     S"
     gene            1546..1839
                     /locus_tag="U2K71_002687"
     CDS             1546..1839
                     /locus_tag="U2K71_002687"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316697.1"
                     /translation="MINLGEWGTIAGSITAIVSLILLVIRPVTASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHTEKLVEHTQQIKTLFRERSR"
     gene            1862..2059
                     /locus_tag="U2K71_002688"
     CDS             1862..2059
                     /locus_tag="U2K71_002688"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEM7316698.1"
                     /translation="MILPDKYYQVIKWTVLTVLPAASVLVATLGKAYGWNGTDMTVLT
                     INAVATFLGVITGVSAYNLKK"
     gene            2070..3089
                     /locus_tag="U2K71_002689"
     CDS             2070..3089
                     /locus_tag="U2K71_002689"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan recognition protein"
                     /protein_id="HEM7316699.1"
                     /translation="MKKKITITAMSLLTALFLLPINGFAYTINNEFNLGPNEGSSQVA
                     NNQYILLHETANETATGRNEAQYMQRSWTSAYTAYIVGDGGIVYQVGQPGYVQYGAGS
                     YANANSPVQIELQHTHDKATFEKNYKAYVELARDSAMKYGIPLTLDTPYNQPGIKSHL
                     WVTQNIWGDHTDPYGYLSEMGVSKEKLAYDLAHGFTDENPTTSDDKPVIDPTRAGAAN
                     PTLTDGTNYAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVKADGIY
                     RPDVNQAYNTLGNVGYHVSFNMRNFPNKKVYVMMRATNDPEGNTKGGAQDFHDKRWYL
                     NIPQR"
     gene            3346..3993
                     /locus_tag="U2K71_002690"
     CDS             3346..3993
                     /locus_tag="U2K71_002690"
                     /inference="COORDINATES: protein motif:HMM:NF014505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PemK/MazF family
                     toxin"
                     /protein_id="HEM7316700.1"
                     /translation="MPNKNYRVYKRGTIVYVDFGVNVGYELSGNHFAIVLNNKDNSKN
                     GVVCVVPISSKKKSNYVSVGKILEVASIKQFISQADKLKEKLRIIILLSLNRGIIDES
                     DLNSFIKDIEAKGEKLSVEEVEKRAVKHNLVCETREQVIKEIKSLSEDMVKYQKVFNC
                     YKKYTQESYVMPLNIQTISKNRIRRINKFDPSGNMKAPGNVLNEVDESLKKYFTK"
     gene            4276..4349
                     /locus_tag="U2K71_002691"
     tRNA            4276..4349
                     /locus_tag="U2K71_002691"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4310..4312,aa:Ile,seq:cat)
     gene            4895..>5463
                     /locus_tag="U2K71_002692"
     CDS             4895..>5463
                     /locus_tag="U2K71_002692"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEM7316701.1"
                     /translation="MCTAITYATKDHYFGRNFDYEMSYNEVVTITPRNYRFDFRKVKN
                     LDKHYAMIGIAAGVSNYPLYYEATNEKGLSMAGLNFPGNADYKELQEGKDNVAPFEFI
                     PWILGQCSTIDEAKELLATINLVNIDFSEKLPLSPLHWLLADKEKSIVIESMKDGLHL
                     YDNPVGVLTNNPPFDYQLFNLNNYRSLSNG"
BASE COUNT         1934 a          853 c         1083 g         1593 t
ORIGIN      
        1 gatgaccctt actggctagg taaagcgcct ttgggtgaga atattgctaa taagtctgtt
       61 acgtttccaa tcaaatctag cgcctacagc ctataccaag ctaacatgga agaagaattt
      121 gttttaggtc aaacctatac aattaccatg aaggcaacca aaccacctat ccaaacgttc
      181 attgtttaca acgaggatag tggagattat agatatggta acttagagcc agtagaaggg
      241 ttagttgata catggggtct gacttttaca ccgcagaagg ttggagttaa ctaccccaaa
      301 cggttaacta ttatccagta tccccaatca acaacaggtg catgtcaaat tgactggctc
      361 aagatcgaaa aaggcgacac acgaaccccg aatattagtc agtttaaata ctttggtgaa
      421 ggcttgaaag acagtaacaa tcccaatgat tacagttggg atgtcacacc tgaatatact
      481 gaaaaaggct tgaatgatgc tgttaatgtg tatgatcctc agagagttga aggtttgaaa
      541 aactttgccg atggtattca aatagcagga gataaagtga tcagtgaaaa tgattgcact
      601 gtttatacat taactaaaga caacagtcaa tcgtttatcg atgggtatgc aacatttatt
      661 aaacacggaa aagaagttat tgtaaatggt acagtaaagt tcaaaaaagc ttatgctttt
      721 ggtgtaccac ttgatgatga agtcccagat gaatttgtcg caaaaattgt tcatggcatg
      781 cttacaggtc cgtcaggaac aagtagtgta tcaaaagcga tgtacgtgcg aaaagactta
      841 ggaacaatta ttacgaatag cgaatttgct gcaaatgaat ggtttacatt ccatggtaat
      901 tattgggtag gggaggaata aaaatgaaaa atatttggaa atacggacga acaggtggag
      961 agtatgctgg acaagtgctt gatgatatgg ttatgactgt tccatttacc gatgttccac
     1021 cactcgaagg aattcgtgct gatggggaac cactaacgat tgctgatcag atgtttgatc
     1081 ctaaactgaa ccaatggatt gttttagtga atgtactaga tcacaacgat ttaaacaatc
     1141 tcaaagcgat gtatgagtcg ttagaaaatg agaacggcga tttaaaacag ctcaacgcca
     1201 aactcatgct aagcgatgta gcgattaaac aggaaaatac tgcattgaaa gaaaaagcgg
     1261 atggtttagc acaaatcaat tcaaaaacga tgcttgcttc gttccaaaat agcaaagaca
     1321 ttgcagaaat taaagagcaa ctaaatccag cttcaaaggg aggtgagtag tatgtttagt
     1381 tttagcgatg tgaaaatgat gtatgattgg ggctgtttta ctgacgatca agttcgacta
     1441 ttcgttccac tatgcattac agacgaagaa gcagaaaaaa tcattaataa agataagagc
     1501 gcatcttaag tgatgcgttt ttatttaagg taaaggagtt gtcacatgat taatttagga
     1561 gaatggggaa caatcgcagg atcaatcact gcgattgttt ctttgatttt attagtaata
     1621 agaccagtca ctgcatcttt ctcgaagatt actgaaactc tttcaaaagt aagtcacaat
     1681 ttagatttgc tgactaaaga tttagaatcg agcaaatcag atcgattgat gattcatgaa
     1741 gaactaaaga aacacgatga aagattagat acacatacag aaaaattggt agaacacaca
     1801 caacaaatca aaactttatt tagagaaaga tctcggtaaa aaagaaagga gttaagaaga
     1861 aatgatttta cccgataagt attatcaagt cattaaatgg acagttttaa cagtattgcc
     1921 agctgcatct gtattagttg caacactagg caaagcgtat ggatggaatg gaacagatat
     1981 gacagtactc actatcaatg cagtagcaac atttttaggc gttatcactg gcgtgtcggc
     2041 ttataatttg aaaaaatagg aggaaacaaa tgaaaaagaa aattactatt actgcgatga
     2101 gcctattaac ggctcttttt ttattgccaa ttaatgggtt tgcctatacg attaacaatg
     2161 aatttaattt aggcccaaac gaaggtagct cacaagtagc aaataatcag tacattttac
     2221 tgcatgaaac ggctaatgaa acagcaacag gacgcaatga agcgcagtat atgcaacgtt
     2281 catggactag cgcttatact gcttatattg tgggagacgg cggaattgtt tatcaagtcg
     2341 gtcaacctgg ttatgtacag tacggtgctg gttcgtatgc taatgcaaat agtcctgtgc
     2401 agattgagtt acaacacaca catgataaag caacgtttga gaaaaactac aaggcatacg
     2461 ttgaattagc tagagattca gcaatgaaat atggtattcc acttacacta gacacaccgt
     2521 ataatcaacc agggatcaaa tcgcatttat gggtaacaca aaatatttgg ggcgatcata
     2581 cagatcctta cggttatctt tctgaaatgg gcgtaagtaa agaaaaatta gcctatgatt
     2641 tggctcatgg atttaccgat gaaaatccga caacttcaga tgataaacca gtcattgatc
     2701 caactcgagc aggtgctgca aatcctaccc tgacagatgg aacaaattac gcccacattg
     2761 atcagttcgg agaaatcgaa aacgcaaact tacatgtggc tggttggcac atcgctaact
     2821 ataaatacga gtatatcttc attatggatt acaatactgg gaaagaatta gctcgagtaa
     2881 aagctgatgg aatatataga ccagatgtaa atcaagctta taatacttta ggaaatgttg
     2941 gctatcatgt atcttttaac atgcgcaact tccctaataa gaaagtctat gtaatgatgc
     3001 gggcaacgaa tgatccagaa gggaacacta aaggcggagc acaagatttt catgataaac
     3061 gctggtattt aaatattccg caacgataaa aatagcccct cgtttttgag gggaagtaca
     3121 taatgcaaat ttgtccataa cttattgatt atcttgtaaa aatgcggtaa tttaatagtt
     3181 agtgagaaga ggtgcttaat tatagttaaa aaagaggaaa tagaagcagc atgtaaccaa
     3241 gcaaatgtta ggtttattgg agaagcttta tctggatatc ctaagtataa atacttacac
     3301 aactggttac aaagtaaaag tttttatctt aataaagaaa gatgcatgcc taataaaaat
     3361 tatagggttt ataaaagagg aactattgta tatgttgatt ttggggtgaa cgtgggttat
     3421 gagttatctg gtaatcattt tgctattgtt ttaaataata aagataattc gaaaaatggt
     3481 gttgtatgtg tagtaccaat ctcttcaaaa aagaaaagta attatgtatc tgtaggaaaa
     3541 atattagaag ttgctagtat taaacaattt ataagccaag ctgataaatt aaaagagaaa
     3601 cttaggataa ttattttact ttcattaaat agaggcatca ttgatgaaag cgatttgaat
     3661 agttttatta aagatattga agctaaaggt gaaaagcttt cagtggaaga agtagaaaaa
     3721 cgagctgtaa aacataattt ggtttgcgag acaagggaac aagtaattaa agaaataaaa
     3781 tcattatctg aagatatggt aaaatatcag aaggtgttta attgctacaa gaaatataca
     3841 caagaatcct atgtaatgcc acttaatatc caaacgatta gtaaaaatag aattagaaga
     3901 attaacaagt ttgatccttc tggaaatatg aaggcacctg gaaatgtcct caatgaagta
     3961 gatgaaagtt taaaaaaata ttttacaaag taaacgatat ttttgttgac atttttctgt
     4021 tcgttagtta ccatgtagtt atagaaggac gtaagcgcct atttttaatg tacacatcgg
     4081 gacgtatgcg cccacataga aacatagttg agagacatct atatggtgtc tcttttttta
     4141 ttaatatgca aaaaagtcta gttttttcaa taatatttat gtaataataa attagccacc
     4201 acaacaaaga atgaaaccca ttattatcta gtctatgtcc attctttttg tttgcagtag
     4261 ttgtgatggc tttccgtact cttagctcag ttggttagag cagacggctc ataaccgtcc
     4321 ggtcgtaggc tcgagtccta cagggtacat actctaagtg atttaaattg cgaataaaat
     4381 ataaaaatga ctcccctttt gactccccca agcagtatat ggatgtctct aaaagttgtt
     4441 tttttagata ataaaaagct gataataagc gaaaaacact taaaatcagc tttaaaccct
     4501 attctcgaaa tccggcgaac cggctaatac cggcgcgcag gttcaaatcc tgtaccttcc
     4561 ttatatcaac gattagaggt tgtgaagaag ctgtcctgca tcaagtaata agaacggcca
     4621 aacaaaaata gcttttccta ttttttcgtt tgattgggct tattaccgca gatgcaagct
     4681 tttgaacacc gtttgttcgg aataaggggc tgtgacaaga cttttgtcac agccccttat
     4741 tgttgtactt ttaacctaca atattttttg attccccctg agtactattt tttataaatg
     4801 ctagtaaatc aatcttcttt ggcctttttt tgataaaaaa aatactagat gtaagataaa
     4861 agtgtaaaaa cgatttgttt taggagggga aacgatgtgt accgcaatca cttatgcaac
     4921 gaaagaccat tattttggaa ggaattttga ttatgagatg tcttataatg aagtggtcac
     4981 tattactcca agaaattatc gtttcgattt tagaaaggta aaaaatttag ataaacatta
     5041 tgcaatgata ggaatagcag ctggggtatc caactatcca ctctattatg aggctacaaa
     5101 tgaaaaaggg ttgagtatgg caggactgaa ttttcctggc aatgctgatt ataaagaatt
     5161 acaagaagga aaagataacg ttgcaccatt tgagtttatt ccatggatac tagggcaatg
     5221 ctcaaccata gacgaagcta aagaattatt ggctactatc aatctagtca atatcgattt
     5281 tagtgaaaaa ctacccttgt cacctttaca ctggttatta gctgataaag aaaaatcgat
     5341 agtaattgaa agtatgaaag atggacttca tttatatgat aatcctgtcg gtgtgttgac
     5401 aaataatccg ccatttgatt atcaattatt taatctgaac aattatcgct cgctatcaaa
     5461 tgg
//
LOCUS       DASHHB010000103         5433 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.108, whole genome shotgun sequence.
ACCESSION   DASHHB010000103 DASHHB010000000
VERSION     DASHHB010000103.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.108"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..177
                     /locus_tag="U2K71_002693"
     CDS             <1..177
                     /locus_tag="U2K71_002693"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316702.1"
                     /translation="QSLVLLAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGV
                     KDVLLNLFNRIVGA"
     gene            190..690
                     /locus_tag="U2K71_002694"
     CDS             190..690
                     /locus_tag="U2K71_002694"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEM7316703.1"
                     /translation="MEQIRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELKGTPIGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCNDMSDVAYYLIVEAGDLGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFELM"
     gene            787..2634
                     /locus_tag="U2K71_002695"
     CDS             787..2634
                     /locus_tag="U2K71_002695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HEM7316704.1"
                     /translation="MVGNKWYKCDLHIHSTASQCFQDKKVTAKEWVEECLNKGLDCVA
                     LTDHNTGENIDEYKKVAIENGLDFFPGVELTCGENGTHLLVLFDKSACTNDISDFLTK
                     MGIKREDWASAAACSMKSVLEVIDFAEHEGLLVIPAHIDEFNGISSAKFGNLGKIFES
                     ENIYAVQTVQKEFFENRSQMIPSSKRDTIYDSVNARYDGRVGKDTLESWYKSVVEAEK
                     NNMTFLSFSDNPHSKGNSKHGLWGIGTRYSYIKMKDEPDLGSLRDALMLGETRVHSDF
                     SNFSINENEVLLEKLSFSGTTLSTKEVVVEFSDNLTSIIGGRGTGKSCITRFLVYVLG
                     KEAELDQFSEIQSDYQNFAQIEHNGSGIFLKDTIVKLNIFYKGTKYQIIRTQDRHSIY
                     EFTQENGLVEKETERLRMISDKVSIYSQKQIYEISKNQTSILELLDGYNSDLISEYKN
                     EIETCVNEIRKLNWDIVSVKKEIQDKAKVELEIEDLKKQVEKLSHKSYKDVYDEYSKE
                     TDIYRELKRDVEALKEIPKNLTDSVDKIDFGNGIKVTDEIDEHRGELIKNLVTNRAKI
                     QTIINEMSDEIDSYRAELNKSDWKVNYNEVSKRYQRGIKNLVKSYLKMN"
     gene            2637..3533
                     /locus_tag="U2K71_002696"
     CDS             2637..3533
                     /locus_tag="U2K71_002696"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM7316705.1"
                     /translation="MLQNLDSLYQSLSKTEERLEEIIQQEIKLKDYQVEVDSAMSRMN
                     KSYQNLYSERSSSSREIFSDITGIEATIQPNRDFNNYVKELRACINKEDTFDSEFGSL
                     IDKLTKNSITNQDIYNDILDIRVSGESSIFSDKKLINSIRKLNDAQVLELQTLKPNDL
                     VVIRLELNGKKVSLSNASAGQKTSAILSMILAYGNSPLILDQPEDDLDSQLINNLIVR
                     SIITKKENRQILIVTHNANIPVNGDSEWLISMGDSKEISADQCGSVDEPNIKPRICTV
                     MEGGEDAFTNRAKRYGFKQIVE"
     gene            3583..3972
                     /locus_tag="U2K71_002697"
     CDS             3583..3972
                     /locus_tag="U2K71_002697"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM7316706.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEEVFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPSTLVRSVNYVSD"
     gene            3959..>5433
                     /locus_tag="U2K71_002698"
     CDS             3959..>5433
                     /locus_tag="U2K71_002698"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM7316707.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELVPYNYSFLSPEQKFQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITSRLKEVAKQRIDIQTDALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKSLKKSFFSGLQEFVYGVNHHLMGDFVSLSNEEI
                     RCYSKLEKLMESKLARRFKVRRVTPSDLTYLIEHIYGEKGIPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPNRCLIEEKPRCLRMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKGSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT         1857 a          786 c         1151 g         1639 t
ORIGIN      
        1 caatctttag tcttactagc gattgtggtc attggtttgt ttttaggatt taaacgcgag
       61 ttttccaaac tgattggctt cttggtgatt gctttgattg cggtaggtct ggtctttaat
      121 gcttctggtg tgaaggatgt cttactcaat ttgtttaacc gaattgtcgg tgcctgaggg
      181 ggaatcacaa tggaacagat acgtgtttat attgcgaacc ttggaaaata caatgaaggt
      241 gagttggtgg gggcatggtt taccccaccc gtcgattttg atgaagtgaa agaacggatt
      301 ggattaaatg acgaatatga agagtacgca attcatgatt atgaactgcc ttttgagatt
      361 gatgagtata caccaattga ggaaattaat cggttgtgtg gtttagcgga agagcttaaa
      421 ggaacgccaa ttggtgaggt cgcttcagag attcaacatg ccttcttcaa ttctttcgag
      481 gaaatggtag agcatgtgga tgatatcatc tattatcccg attgtaatga catgagtgat
      541 gtagcgtatt acttgattgt agaagctggt gatttaggcg aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtcga gacttggaga ttggcgggaa ttatttggtt
      661 acgagtcgag gggtttttga actaatgtaa atcatttgtt agaattgacc agaaatttct
      721 atcgtacttt tacttttcta tttgatatac tgaaatagaa aatatttgtt gataggggtg
      781 ttactaatgg ttggcaataa gtggtacaaa tgtgatcttc atattcattc aactgcgagt
      841 cagtgctttc aggataaaaa ggttactgct aaggaatggg tagaagaatg tttaaacaaa
      901 ggactcgatt gtgttgcatt aactgatcac aatacagggg aaaatattga tgaatataag
      961 aaagtggcta tcgaaaatgg cttagatttt tttcctggag tggaattaac gtgtggagaa
     1021 aatggaactc atttactagt cctatttgat aaaagtgcat gtacaaatga tatctctgat
     1081 ttcttaacca agatgggtat aaaaagagag gattgggcca gcgctgcagc ttgctcaatg
     1141 aaatcagttt tagaagttat tgattttgct gagcacgagg gcttgctcgt tattcctgca
     1201 catatagatg agtttaatgg aatttcttca gcaaaatttg gaaatctagg aaagatattt
     1261 gaatcagaaa atatatatgc agtacagacg gttcaaaaag aattttttga aaatcgctca
     1321 caaatgattc cctcatctaa gcgtgacact atctatgata gtgtgaatgc cagatatgat
     1381 ggtagagttg gaaaagatac tttggaatct tggtataaat cagttgttga ggcagaaaaa
     1441 aataatatga catttcttag cttttctgat aacccacact cgaaaggaaa ttcaaaacat
     1501 ggactttggg gaataggcac aagatattcc tatataaaaa tgaaggacga acctgacttg
     1561 ggatcacttc gcgatgcttt aatgctaggc gaaactcgag ttcattctga tttttctaat
     1621 ttctccatta atgaaaatga agttttattg gaaaaattaa gcttttctgg gaccactctt
     1681 agcacaaaag aagttgtagt cgaattcagc gataatttaa cttcaattat tggaggaaga
     1741 ggaacaggca aatcttgtat tacaagattc ttggtctatg tccttggaaa ggaagcagaa
     1801 cttgatcaat tttctgaaat acaaagtgat tatcagaact ttgcacaaat tgaacataat
     1861 ggctctggaa tatttttgaa ggatacaatt gtgaaactga atattttcta taaggggaca
     1921 aaatatcaaa taataagaac acaggataga cattctattt atgaatttac acaagagaat
     1981 ggtctggttg aaaaagaaac agaacggttg agaatgattt ccgataaggt gagtatatac
     2041 tctcaaaaac aaatttatga aatttcaaaa aatcaaacta gtatactaga attactagat
     2101 ggatataact ctgatttgat ttcagagtat aaaaatgaga ttgaaacatg tgtaaacgaa
     2161 ataagaaaat taaattggga tattgtttct gtgaaaaaag aaatacaaga taaggcgaag
     2221 gtagagcttg aaattgaaga tttaaaaaaa caagtagaaa agctatctca taagagttat
     2281 aaagatgttt atgatgagta ttcaaaagag acagatattt atagggaact gaaacgtgat
     2341 gtggaggcac ttaaggaaat tcccaaaaat ctaactgata gtgttgacaa aattgacttt
     2401 ggcaatggta taaaagttac agacgaaatt gatgaacacc gcggagaatt gattaagaat
     2461 ttagtgacca atcgggctaa gattcaaaca ataattaatg aaatgtcaga tgaaattgat
     2521 agttatagag cagagctgaa taaatcagat tggaaagtta actataatga ggtttcaaag
     2581 aggtatcaaa gaggtatcaa aaacttagtg aagagttatc tcaagatgaa ttaaaaatgt
     2641 tacaaaacct tgattcttta tatcaatctt tatcaaaaac tgaagagcga ttggaagaaa
     2701 ttattcaaca agaaataaag cttaaagatt accaagttga ggttgactca gcaatgtcaa
     2761 gaatgaataa atcatatcaa aatctctatt ctgagagaag tagttcctcg cgcgaaattt
     2821 tttctgatat tacaggaata gaggctacta ttcaaccaaa tagggatttc aataattatg
     2881 ttaaagagct cagagcttgt attaataagg aggacacttt tgacagtgag tttggatctc
     2941 ttattgataa gctgacaaaa aattccatta ctaatcaaga tatatataat gacatacttg
     3001 atataagagt tagtggggaa agtagtattt tttcagataa aaaattaatc aattctataa
     3061 gaaaattgaa tgatgcacaa gttcttgaat tacaaaccct taagccaaat gatttagtag
     3121 ttatccgact tgaactaaat ggaaaaaaag ttagtttatc gaatgctagt gctggtcaaa
     3181 agacatctgc aattctttct atgattttgg cgtatggaaa ttcaccactc atattggatc
     3241 agcctgaaga tgatttggat agtcaattaa ttaataactt aatcgtaaga agtatcatta
     3301 caaaaaagga aaacagacaa attttaattg taactcataa tgcaaatatc ccagttaatg
     3361 gtgattcaga atggttgatt agcatgggag attcaaaaga aatatcggct gatcagtgcg
     3421 ggtcggtaga tgaacccaat atcaagccaa gaatatgtac tgtgatggaa ggtggcgagg
     3481 atgcttttac aaatagagcg aaacgctatg gttttaaaca aattgtagaa taacactaga
     3541 gggtgagaca tattattatg tccgcccttt ttaggaggaa aaatgaaaaa aataaaaagc
     3601 tataccagta tctgggcagt tgaaaaagtc atttacgcaa tcaatgattt tcagttgcct
     3661 tttcctgtga cttttaacca aatggcttgg ttcgttttat ctttactgtt tgtcatcgta
     3721 tttgcccatg tgcctccact atctatgata gaggaggtct ttttaaaata cttaggcata
     3781 ccggtggctg tcacttggtt tatgtcgcaa aagacgtttg atggcaagaa accactggga
     3841 tttttacgat cctttatcag ctatcacttg cgatttaagg tgacttttgc aggaaagaaa
     3901 gtcaaagaac agaagaaacg atgggatgaa cccagtactc ttgtaaggag tgtgaactat
     3961 gtatccgatt aagtatattg aaaataatct cgtctttaac caagaaggcg agtgttttgc
     4021 ctactatgaa ttagtgcctt acaattactc ctttttgtca cccgaacaga aatttcaagt
     4081 gcatgataac tttcgccaac taattgcgca aaatcgtgaa ggcaagatcc atgcgttaca
     4141 aattgccaca gaaagtagta ttcgcgccac acaagaacga tcaaaaaaag aaattaccag
     4201 tagactcaaa gaagtagcga aacaacgaat tgatatacag acagatgcat tagtatccat
     4261 gattggcgat agtcagattg attatcgctt ttttattggc tttaaactaa ttgcgacaga
     4321 tgaagaagtc aatctaaaga gtctcaagaa atctttcttt tctgggcttc aagaatttgt
     4381 ctatggagtg aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg
     4441 ctgctattct aagcttgaaa aattaatgga aagcaaattg gctcgaagat ttaaagtcag
     4501 gcgagtcaca ccgagtgacc tgacgtattt gattgaacat atttatggcg aaaaagggat
     4561 accgtttgaa gaatacgaat ttcagctacc aaagaaaaag ctaaaatcag aaacactggt
     4621 gaaacgttat gacttattac gtccaaatcg ttgtttaatt gaagaaaaac caaggtgctt
     4681 acggatggaa catgagaatc atgagtcgta cgtagcgtat ttaacaatta atacaattgt
     4741 gggggagatg gaatttcctt cctcagaact tttctattac cagcaacaac aatttacatt
     4801 cccaatcgac acaagtatga atgtagagat tgtgacgaat aaaaaggctt tagctaccgt
     4861 tcgtaataag aaaaaagaac tgaaagattt agacaatcat gcttaccaat ctgacaatga
     4921 aacgaattcc aatgtcttag atgctttgga ttccgtggat gaattggaaa ccaccttgga
     4981 tcagtcaaaa gaatctatgt acaaactgag ttatgtggtt cgagtgagtg ccgaatcagt
     5041 ggatgaacta aagcgacgct gtgatgaagt gctcgatttt tatgatgata ccaatgtaaa
     5101 actagtccgg ccctttggcg atatgatggg gctacatgaa gaattcttac cctcaagtaa
     5161 acgatatatg aacgactata ttcagtatgt tacgtctgat tttttggcgg ggcttggttt
     5221 tggggctact cagatgctag gagaattaga agggatttac tttggctaca atgtggatac
     5281 aggtcgtaat gtttacctga aaccggcatt ggcttcacaa ggagtcaagg gttcagtaac
     5341 caatgcgctg gccgccgctt tcctgggttc tcttggtgga ggtaaatcct ttagtaacaa
     5401 tctcttagtc tactatgctg ttttgttcgg tgg
//
LOCUS       DASHHB010000104         5310 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.109, whole genome shotgun sequence.
ACCESSION   DASHHB010000104 DASHHB010000000
VERSION     DASHHB010000104.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5310)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5310)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5310
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.109"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(71..1219)
                     /gene="nagA"
                     /locus_tag="U2K71_002699"
     CDS             complement(71..1219)
                     /gene="nagA"
                     /locus_tag="U2K71_002699"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HEM7316708.1"
                     /translation="MRTFIYADKFFLASGVKHAGYLEITDGLFGEYTKELPEGEVNII
                     DQSGKWIAPGLVDTHIHGYMNHDVMDNDAEGIKVMSEGLLSCGVTSFLPTTLTSSKER
                     LKDVAETIGKMYQEAPGAKIKGIYFEGPFFTEEHKGAQNPSYFGDPDLDTFHEWQEAS
                     GGLIKKIALAPERQGVEEFVKQVTEEGVVVSLGHSNATLEEAQKAVEAGASVFVHAFN
                     GMRGLNHREPGMVGALLSLEHVFSELICDGHHVHPNAAGILMEKAGHDHVALITDCMM
                     AGGMPDGNYNLGEFPVVVKEGTARLESGNLAGSILKLKEAIKNVVDWGLATPEQAVMM
                     ASLIPAISCKIDDTCGKIAQGRDADFIVLNPDMTLEATYLDGVERFHA"
     gene            complement(1295..2131)
                     /locus_tag="U2K71_002700"
     CDS             complement(1295..2131)
                     /locus_tag="U2K71_002700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7316709.1"
                     /translation="METLIESFGTIIKEIRKEQKMTQKMLSHDICSQSVLSRIENNEE
                     LPNVLVMMQICQRLGVTIDHVMNLSRKTTRTNDKLFELLDSCFQKRDYRKLEQVLKSS
                     DISENLYQATDFQRYYYYLGVCEFTLRQNISQSLYYLKEALSYSSQKERIHVSDTEIQ
                     LISCIGKIYGVAGKTAEARYYLSRSIQLLHQSADERSKSEFSQIFYNYGNFLFHQNEI
                     DEALEQVNQGIYWAQEKNSYYYLNDLFVLKSLIYKRKEEPERAAFYEGLAQAVKQISK
                     NL"
     gene            complement(2315..3532)
                     /locus_tag="U2K71_002701"
     CDS             complement(2315..3532)
                     /locus_tag="U2K71_002701"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002357817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM7316710.1"
                     /translation="MREFEKSNKLEGVSYDVRGPVLEEAERMQEEGISILKLNTGNPA
                     PFGFEAPNEVIRDLIMNARDSEGYSDSKGIFSARKAIEQYCQLKHFPNVTINDIYTGN
                     GVSELITMCMQGLLDNGDEVLVPMPDYPLWTASIALAGGTPVHYICDEEAEWYPDIDD
                     IKSKITSRTKAIVIINPNNPTGALYPKELLEEIVEVARQNNLIIYSDEIYDRLVMDGL
                     EHIPIATLAPDLFVVTLNGLSKSHRVAGFRVGWMVLSGDKSHVKGYIEGLNMLSSMRL
                     CSNVLSQQIIQTALGGYQSVDELLLPGGRIYEQREYIYNAINDIPGLSAVKPKAAFYI
                     FPKIDTKRFNILDDEKFVLDFLHEHHILLVHGGGFNWNQPDHFRIVYLPKMDDLKQTA
                     KKMREFLATYRQK"
     gene            complement(3681..3860)
                     /locus_tag="U2K71_002702"
     CDS             complement(3681..3860)
                     /locus_tag="U2K71_002702"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316711.1"
                     /translation="MGGLIKMEKYDGEFSGLGMILGVLIGLAFGRFLLGFMLGIICGI
                     AMDWAANLWNDYHDN"
     gene            3915..3998
                     /locus_tag="U2K71_002703"
     tRNA            3915..3998
                     /locus_tag="U2K71_002703"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3949..3951,aa:Leu,seq:gag)
     gene            complement(4238..4816)
                     /locus_tag="U2K71_002704"
     CDS             complement(4238..4816)
                     /locus_tag="U2K71_002704"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316712.1"
                     /translation="MNILFLLLFFLIFLLTYRKCQNYYVRILTALPFFLNLIIYFGNT
                     MGQSFTYVNGNKRAMIWSSSNLNNLFTELGTKLSLFYPGTWIATLVVLALLLCLIVGI
                     YLSFDNKKTSIFLVILMIMGFCSRLIMGFSPTVWASGMRTYYILYVVIAILVLMAVKE
                     LMKSMSVQKNEFMQFGLTVLGICTFIITVINR"
     gene            complement(4822..5205)
                     /locus_tag="U2K71_002705"
                     /pseudo
     CDS             complement(4822..5205)
                     /locus_tag="U2K71_002705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347134.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1654 a         1082 c          838 g         1736 t
ORIGIN      
        1 ttactagaac acaaaaaaaa ccttaaaacc aaacttaaga agtttagttt taaggttaac
       61 ttaaattata ttatgcgtgg aaacgttcca cgccatctag ataagttgct tccaatgtca
      121 tatcagggtt caatacgata aagtctgcat cgcgtccttg agcgattttt ccacatgtat
      181 catcgatttt acagctgatt gctggaatca gactagccat catgactgct tgttctggtg
      241 ttgctaatcc ccagtctaca acatttttaa ttgcttcttt caatttcaat atacttccag
      301 ctaagttgcc agattctaga cgtgctgttc cttcttttac tactactgga aattcgccta
      361 aattgtaatt tccatcaggc atcccaccgg ccatcataca gtctgtaatt aatgcgacat
      421 gatcatgtcc tgctttttcc attaggattc ccgcagcatt tggatgtaca tgatggccat
      481 cacaaatcaa ttcagaaaag acatgttcta aagataataa cgcacccacc atacctggtt
      541 cgcgatgatt caatccacgc ataccattga aagcatggac aaatacactt gcgcctgctt
      601 caacagcttt ttgcgcttct tctagtgtag cattactgtg tcctagagaa acaactactc
      661 cttcttcagt cacttgtttg acaaactctt ctacaccttg acgttccggt gctaaagcaa
      721 tttttttgat taaaccacct gaagcttctt gccattcatg gaaagtatcc aaatcaggat
      781 ccccgaaata acttgggttt tgtgcccctt tgtgttcttc tgtgaagaaa ggaccttcaa
      841 aatagattcc tttgatttta gctcctggtg cttcctgata cattttgccg attgtctcag
      901 ctacatcttt taaacgttct ttgcttgacg tcaatgttgt tggtaaaaat gaagtcactc
      961 cacaagataa cagaccttca gacatcactt tgatgccttc tgcatcatta tccattacat
     1021 catgattcat atagccgtga atatgtgtat ctaccaaacc aggagcaatc catttgccgc
     1081 tttggtcaat gatgttgact tctccctcag gtagttcttt ggtgtattca ccaaaaagtc
     1141 catcggtaat ttcaagatag cctgcatgtt tgacacctga cgctaaaaag aatttgtcag
     1201 catagataaa agttctcatt ctattctctc cctttgtttt tctacctcta aagtactctt
     1261 atttagagaa gaagtcaaga atggtctaca ccatttacaa attttttgaa atttgtttca
     1321 cagcttgtgc taagccttca tagaatgctg ctctttctgg ttcttcttta cgcttgtata
     1381 ttaagctttt aagcacaaat aaatcattga ggtaatagta gctattcttc tcttgagccc
     1441 aatagattcc ttgattcacc tgttctaacg cttcgtcgat ttcattttga tggaataaga
     1501 aatttccata attataaaaa atttgcgaaa attcactttt tgatcgttca tcagctgatt
     1561 gatgcagcag ttggatgctt cgactcaaat agtagcgtgc ttcagccgtt tttcctgcca
     1621 caccatatat ttttccgata caactaatca gttgtatttc tgtgtcagaa acatggattc
     1681 tttctttttg gctagagtag cttaatgctt ctttcaaata ataaagggac tgagagatat
     1741 tttgacgcaa cgtgaattcg caaacaccga gataataata atagcgttga aagtcagttg
     1801 cttgatacag attttcagaa atatcagaag attttagtac ttgttctaac ttccgataat
     1861 cacgtttttg gaaacaacta tccaacagtt caaataattt atcgtttgtc cgagtagttt
     1921 ttcgagagag attcatcaca tgatcaatcg ttacccctag ccgttgacaa atctgcatca
     1981 tcaccaatac attcggcagt tcctcattat tttcgatccg acttaataca ctttgtgaac
     2041 aaatatcatg cgaaagcatt ttttgcgtca ttttttgttc ttttcgaatc tctttaatga
     2101 tcgttccaaa ggactcgatt aacgtttcca taagtatcct ccccaaaata tgcgtattcg
     2161 cattctattc gtattttact ttaggaagtc gcaaacttca agaaaagact tgcaactaaa
     2221 taatatactt tagttattta agaaaactcc actagaaaga gagcctcagt gtgtttgtct
     2281 gtggccctcc tactgaaaaa tttggttttt aaaatcattt ttggcgatag gttgctagga
     2341 attctctcat tttcttagct gtttgtttca aatcatccat tttaggaaga taaacgattc
     2401 gaaaatgatc tggctggttc caattaaatc caccaccatg tacaagtaga atatgatgtt
     2461 catgtaaaaa gtccaaaacg aatttttcat catccaaaat attgaatcgc tttgtatcaa
     2521 tttttgggaa tatataaaaa gcagcttttg gttttacggc tgaaagacca ggaatatcat
     2581 tgattgcatt atagatatat tctctttgtt catagatcct tccgcctggc aggagcagct
     2641 catcgacact ttgataaccg cctagcgcgg tttggataat ttgctgagac aatacattgg
     2701 aacacaaacg catggaggac aacatattca gcccttctat atagcctttg acgtgtgact
     2761 tatctccact taggaccatc cagcctactc gaaatcctgc tacacgatga gatttggata
     2821 gaccgttcag cgtaacaacg aacaaatcag gcgctaatgt agcaatcgga atatgttcta
     2881 gaccatccat cactaagcga tcatagattt cgtcggaata aataatcagg ttattttgtc
     2941 gagctacttc aacgatttct tctaataatt cttttggata aagtgcccca gtaggattat
     3001 taggattaat gataacaata gcttttgtcc gactcgttat ttttgatttg atatcatcaa
     3061 tatcaggata ccattctgcc tcttcatcgc aaatataatg aacaggtgtg cctccagcta
     3121 atgcaataga agccgtccac aacggatagt ccggcattgg aaccagtact tcatctccat
     3181 tgtccagcaa cccctgcata cacatcgtga tcaattcgct cactccgttg ccggtgtaga
     3241 tatcattgat cgtcacattc ggaaaatgtt tcagttggca atattgctca attgcttttc
     3301 tagcagaaaa gatccccttg gaatctgagt agccttctga gtcacgggca ttcatgatca
     3361 aatcacgaat gacttcgttt ggtgcttcaa atccaaaagg tgcgggattt cccgtgttca
     3421 attttaaaat actaatgccc tcttcttgca tccgctccgc ttcttctaga acgggaccgc
     3481 gaacatcgta actgacacct tctaatttgt tggatttttc aaattctctc atcacttagc
     3541 caatccctca tttcaaaaag ttagaacttt ccaaattttt tgttatttac tatttcaata
     3601 tcatactcca tcctagaaaa ttttgccact tatttaggaa aaacgtaagt attaaaaaaa
     3661 cacaaacaag cgttgagaac ttaattgtca tgatagtcat tccatagatt cgctgcccaa
     3721 tccattgcaa ttccacaaat gattcctagc ataaaaccaa gcaaaaaacg tccgaatgca
     3781 agtccaatca atacacctaa gatcattccc agtcctgaaa attctccgtc atatttctcc
     3841 atcttaataa gtcctcccat ttttgagctt gacaactatg taaaacctcg ctatcattag
     3901 tgtgttatta ttacgcagat gtgttggaat tggcagacaa gcatgattga gggtcatgtg
     3961 agcgttagct cgtgtgggtt cgagtcccat catctgcact acaactcgtg aagttaattt
     4021 tgcttcacga gtttttttgt ttgaacgttt ctttatgcca ttatatacat atcactgtat
     4081 cctttacaaa aagaacgtac aatcatgtaa acagcctgag gtgagtatat gaaggtcgga
     4141 aacaagctac tcgtagaaac ttgcaagtgt taaaacaatt aatcgaaaaa cgagtaaaat
     4201 ccctcaccta atactaaagc gagggatttt ttgtatatta tctgtttatc accgttatta
     4261 taaaggtaca aataccaagc acggttaatc caaattgcat aaattcattt ttttgtacac
     4321 tcatgctttt cattagctct ttaactgcca ttagtactaa gatggcgata acaacataaa
     4381 gtatatagta cgtccgcatt ccggaagccc atactgtggg agaaaatccc ataattaacc
     4441 ttgaacaaaa tcccattatc atcaaaataa ctaaaaaaat agaagttttc ttattgtcaa
     4501 aacttaaata aattccaaca attaaacata gtaataatgc caagactact aaagtagcta
     4561 tccaagtacc tggataaaaa agtgacagtt tcgttcctaa ctctgtaaag agattattca
     4621 ggttactact actccaaatc attgcccttt tatttccatt gacgtaagta aagctttgac
     4681 ccattgtatt accaaaataa ataatcaggt ttaagaaaaa cggtaatgca gtcaatatac
     4741 gcacataata gttttgacat ttcctatatg ttagaaggaa aattaagaaa aatagcaata
     4801 aaaataagat attcatgtct aaaaaaacgg ctttccaaat gatgaaatac ctaaatctaa
     4861 cttattaaga aagttgaaat ttttatattc aggaaaccat ttattaattt catgtgcagc
     4921 acgtaatgca ttccctggag ttgtcaaaga gaaaattaat tctgctaagc ttattattga
     4981 aaatactgat aacttataat tatatcgtcc attgacaatc aaatataaac taactatact
     5041 tgttagaaca aaaaaacagg cgttgacttg ttcttgatta gttaaaaata tcaataaagg
     5101 aatacttacc caatatattt ttctatttat ttcttctcct cttaagagtt gaaaaaacgg
     5161 ataaaaagtt agtaaactaa atgctactgg ccactgatag ttcaaggtgg ttgctctcca
     5221 accggtttcc cccattactg tcaatggtat taaacagtat atagagcaaa ccaataataa
     5281 atttttaaca tttattttct caaaaacata
//
LOCUS       DASHHB010000105         5247 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.111, whole genome shotgun sequence.
ACCESSION   DASHHB010000105 DASHHB010000000
VERSION     DASHHB010000105.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5247)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5247)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5247
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.111"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(207..1169)
                     /locus_tag="U2K71_002706"
     CDS             complement(207..1169)
                     /locus_tag="U2K71_002706"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004233175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HEM7316713.1"
                     /translation="MVAKLTDVAELAGVSPTTVSRVINNKGYLSEKTKQKVHEAMKTL
                     GYKPNNLARGLQGKSPQLIGLIFPNISNIFYSELIEYLEIELFKHGYKTIICNSQNDP
                     AKEREYIEMLEANQVDGIISSSHNLGIADYERVRAPIVAFDRNLAPNVSIVSSDNFEG
                     GKLAAKTLQKNGCQNTIMITGNDNTDSPTELRALGFSFQNPNGKIFKIPNNLSTIRRE
                     MEIKSTIINHKPDGIFVSDDLTAILTMKIAHQLKLNIPEDLKIIGYDGTSFIEKFFPQ
                     LTTIRQPIDEIASLIVDILLKKIKGEKTNKDYILPISILSGGSI"
     gene            complement(1171..2610)
                     /locus_tag="U2K71_002707"
     CDS             complement(1171..2610)
                     /locus_tag="U2K71_002707"
                     /EC_number="3.2.1.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sucrose-6-phosphate hydrolase"
                     /protein_id="HEM7316714.1"
                     /translation="MNLPREIRYRAYSDWTKDEMDKIKDNVKRSPWRASYHIEPKTGL
                     LNDPNGFSFFNGKYTLFYQNWPFGAAHGLKEWVHNESDDLVHFHVTGAELLPDTKRDS
                     HGAYSGSAYEIEGNLFLLYTGNVRNENWERDTLQIGAWMDKDYNITKGENVLIHKPAD
                     VTDHFRDPQMFNYEGQLYAIVGAQNLEKSGFIKLYKADDNNVETWEEVGNLDFGGTGS
                     EYMIECPNLVFVDKKPVLIYCPQGLDKSELNYGNIYPNTFKICQSLDIEKVKLVGASE
                     IQNLDYGFEAYATQGFNTPDGRALIVSWIGLPDIDYPTDKYDYQGAMSLVKELSIKDG
                     KLYQYPVEAITSLRASSEQFTAKAETNNTYELELTFPPNQKSELLLFSDSKGNGLSLI
                     VDTKEGKIILDRSKAGIQYATDFGTIRECSIDTKETTANIFVDNSIIEIFINKGEKVF
                     TSRVFPENHQNGIQIKSGEPTGKYFELKY"
     gene            2795..4741
                     /locus_tag="U2K71_002708"
     CDS             2795..4741
                     /locus_tag="U2K71_002708"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS beta-glucoside transporter subunit IIBCA"
                     /protein_id="HEM7316715.1"
                     /translation="MNYSKVASDVIQAVGKDNLIAAAHCATRLRLVLKDDTKVNQKAL
                     DENPDVKSTFKIDGQYQVIIGAGDVNFVYDELIKKTGLSEISTDDLKQIASKDKKFNP
                     IMALIKLLSDIFVPIIPALVAGGLLMALQNFLTAAGLFGPKSIEEMYPAIEGISSMIQ
                     LMSAAPFMFLPILVGISAAKRFGANQFLGAAIGMIMTTPDLGGSTEYWNIFGYHVSQT
                     NYAYQVIPVLAAVWILSILEKFFHKKLPSSIDFTFTPLLSVMITGFLTFTVIGPVMLL
                     LSNGITDIIVWLYNTTGFIGMGIFGGTYSLIVMTGLHQSFPAIETQLLSAWTNGIGHG
                     DFIFVVASMANVAQGAATFAIWFLTKNSKTKSLASSAGVSALLGITEPALFGVNLKYR
                     FPFFCALIGSGIATAVAGLLKVVAVSLGSAGFLGFLSINATSIPFYVLCELISFVVAF
                     AITYFYGKTKAAGIFKAEEIAEQTPVNSSKVATTDPISTDVESTQTKVSSETIVSPIS
                     GKVISLSSVNDPVFSSEAMGKGIAIKPNGNTVHSPVDGTIQLTFETGHAYGIKSNNGA
                     EVLIHIGIDTVSMGGKGFTQKVTTNQKVRKGDILGAFDRSMIEKEGLDDTTMIIVTNT
                     SDYSEVNIVAENTVTEGTDLLEIK"
     gene            5005..>5247
                     /locus_tag="U2K71_002709"
     CDS             5005..>5247
                     /locus_tag="U2K71_002709"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM7316716.1"
                     /translation="MLYGAIEAGGTKFVCAIGDEEMTIKERVSFPTTTPEETMPLVID
                     FFKQYQADLAGIGIGSFGPIDIHRDSATYGYITSTPK"
BASE COUNT         1696 a          888 c          868 g         1795 t
ORIGIN      
        1 cgtccaattt cgtaaaacaa ccgtaacagt tgtgtaaaaa tatcatttta gagcatgaaa
       61 aaaggagtgg atttctccac tccaaaaaag tctaactttt gggggtcact acaagttgac
      121 aactcctttt tgtttacaaa tcaattggat atctacaaat tgtattttct aacgaatttt
      181 atagacatta aaaaattaat ttaacgttaa atacttcctc cagaaagtat tgagattggt
      241 aaaatataat ccttatttgt cttttcgcct ttaatttttt tcagaagaat atcaacaata
      301 agacttgcaa tttcatcaat tggttgtctt attgttgtca attgtgggaa aaatttctca
      361 ataaaagagg taccatcgta tccaataatt tttaaatctt ctgggatatt aagtttaagt
      421 tgatgagcaa ttttcatagt gagaatggct gttaaatcat cagaaacaaa aataccatct
      481 ggtttgtgat ttataatagt tgactttatt tccatttcac gacggatagt agacagatta
      541 ttaggaattt tgaaaatttt tccatttgga ttttgaaaag aaaaacctag tgcacgtaat
      601 tctgtgggag aatccgtgtt atcatttcct gtaatcataa tggtattttg acaaccattt
      661 ttttgtaaag tcttagctgc aagttttccc ccttcaaagt tatctgaaga aacaatggaa
      721 acattaggag ctaaatttct atcaaaagca acaataggtg ctctcacacg ctcataatca
      781 gcaattccaa gattatggct tgatgagatt atgccatcaa cttggttggc ttcaagcatt
      841 tcaatgtatt ctctctcttt agcaggatca ttttgactat tgcaaataat cgttttatat
      901 ccatgtttaa ataactctat ttcgagatac tcaatgagtt cagagtaaaa gatattacta
      961 atattaggaa aaataagacc aataagctga ggtgactttc cttgaagtcc acgtgctaaa
     1021 ttatttggtt tataaccaag agttttcata gcttcatgaa ctttttgttt tgttttttct
     1081 gataaatatc ccttattatt aatgacgcgt gaaacggttg ttgggctaac tccagctaat
     1141 tcagcgacat cagttaattt tgctaccatt ttaatatttt aattcaaagt attttccagt
     1201 aggttcgcct gatttgattt gaataccatt ttggtgattt tcagggaata cacgactagt
     1261 aaacactttt tctcctttat taataaaaat ctcaatgatt gagttatcta caaaaatgtt
     1321 agcagttgtt tctttagtat caattgagca ttcacgaatc gtaccaaagt cagtggcata
     1381 ttgaatgcct gctttgctgc gatctaaaat aatttttcct tctttggtat ctacaatcaa
     1441 gctaagtcca tttcctttac tatcagagaa aagaagtaac tccgatttct gatttggagg
     1501 aaaagttagt tcaagctcat aagtattatt tgtttctgct tttgctgtaa attgttcaga
     1561 actagcacgt aatgatgtaa tagcttctac tggatattgg taaagtttac catcttttat
     1621 tgaaagttct tttacaagac tcattgctcc ctgataatca tacttatcag ttggataatc
     1681 gatatcaggt agtccaatcc aactgacaat cagagcacga ccatcaggag tattgaatcc
     1741 ttgagtcgca tacgcttcaa atccataatc aagattttga atttctgaag cgcccacaag
     1801 ttttactttt tctatgtcaa gcgattgaca gatcttaaaa gtattcggat agatattccc
     1861 ataatttagc tcagatttat ccaacccttg aggacaataa atgagaacag gttttttgtc
     1921 tacaaatact agatttggac actcaatcat gtattctgaa ccagtgccgc caaaatcaag
     1981 gttgccaact tcctcccatg tttccacgtt attgtcatcc gctttgtaaa gtttgataaa
     2041 gcctgatttt tctaaatttt gtgctccaac aatcgcatac aattgtcctt cataattaaa
     2101 catttgtgga tcacgaaaat gatcagttac atcagcaggt ttatgaataa gaacattctc
     2161 acctttcgta atgttgtagt ctttatccat ccatgctccg atttgaagtg tatcacgttc
     2221 ccaattttca tttcgaacgt ttcctgtata gagtaagaat agattcccct ctatttcata
     2281 tgcagaaccg gaataagcgc cgtggctatc tcgttttgtg tctggaagaa gttctgctcc
     2341 tgttacatga aaatgaacta aatcatctga ttcattgtgt acccattctt tcaacccatg
     2401 agcagctcca aatggccaat tttgataaaa aagcgtatat tttccgttaa aaaaagaaaa
     2461 accattagga tcgtttaaca atcctgtttt aggttcgata tgatagctag cacgccaagg
     2521 tgatcgtttt acattatcct taattttatc catctcatct ttagtccaat cagaataagc
     2581 acgatatcgt atttctcgag gtaagttcat attttactcc ttcaatttat ctttttacaa
     2641 ctagtatggt aaacgttttc cgttgaaaaa tcaatcattt catttaaaaa aataaactat
     2701 atgacaaacg tttgacatat ttttaagata tgctaaaata aatttgcatt tcagtgaacc
     2761 gttttcacgt aaaaataaaa aaggagtgat tcgaatgaat tacagtaaag tagcttccga
     2821 cgtaatacaa gcggtcggaa aagataacct tattgcagct gcacactgcg ctacacgtct
     2881 tcgtctcgta ctaaaagatg acacgaaagt gaatcaaaaa gctttagacg aaaatccaga
     2941 tgtgaaaagc acatttaaaa ttgatggaca atatcaagtg attatcggtg caggtgatgt
     3001 taatttcgtt tatgacgaac ttatcaaaaa aactggactt tcggaaattt caacagatga
     3061 tttaaaacaa attgctagta aagataaaaa attcaacccc attatggcat taattaaatt
     3121 attatcagat atttttgttc caattatacc tgcccttgtt gcaggtggtt tattaatggc
     3181 attacaaaac tttttaacag ctgctggact ttttggacca aaatcaattg aagaaatgta
     3241 tcctgcgatt gaaggtattt cctcaatgat ccaactgatg tccgctgcac cattcatgtt
     3301 cttaccaatt ttagtaggta tttcagctgc taaacgtttt ggagcaaacc aatttcttgg
     3361 tgctgcaatt ggtatgatca tgacaacacc agatcttggt ggctcaactg aatattggaa
     3421 tatttttggt tatcatgttt cacaaacaaa ctatgcttat caagttattc cagttttagc
     3481 agctgtttgg atattatcta tattagaaaa attctttcat aaaaaattgc catcatctat
     3541 tgactttaca ttcacgccat tattatcagt aatgattact ggatttttga ctttcacagt
     3601 tattggccct gtaatgttat tgttgtctaa tggaattaca gacattattg tttggctata
     3661 taacactact ggatttattg gaatgggtat ttttggagga acatattcac ttatagtcat
     3721 gactggttta catcaatcat ttccagctat tgaaactcaa ttactttcag cgtggacaaa
     3781 tggtatcgga catggagact ttatctttgt agttgcatct atggcaaacg ttgctcaagg
     3841 tgctgcaaca tttgccattt ggttcttaac taaaaattct aagacaaaaa gtcttgcttc
     3901 ctctgcgggt gtctcagcac ttcttggaat tacagagcct gccttgttcg gagttaactt
     3961 aaaataccgc tttccattct tttgtgcttt aattggttca ggaattgcta cagcagtagc
     4021 aggattactt aaagtagttg ctgtttcact tggttcagca ggtttcttgg gatttctttc
     4081 aattaatgca acatctattc cattttacgt attgtgtgaa ttgattagct ttgtagttgc
     4141 ttttgcaatt acttacttct acggtaaaac aaaagctgct ggtatcttta aagcagaaga
     4201 aattgctgaa caaacaccag ttaattcttc aaaagtagca acaacagatc caatatctac
     4261 tgatgttgaa tcaacacaaa caaaagtttc atcagaaaca attgttagtc ccatttctgg
     4321 taaagtgatt agtctttctt cagtaaatga tccagtcttt tcatcagaag ctatgggaaa
     4381 agggattgcc attaaaccaa atggaaacac ggtccattct ccagtagatg gtactattca
     4441 actcacgttt gaaactggac atgcttatgg tattaaatca aataatggtg ctgaagttct
     4501 aattcatatt ggtattgata ctgtatcaat gggaggaaaa ggatttacac aaaaagttac
     4561 tactaatcaa aaagttagaa aaggcgatat tcttggagca tttgaccgtt caatgattga
     4621 gaaagaaggt cttgatgata caacaatgat tattgtgacc aatacttcgg attattcaga
     4681 agtaaatata gtagctgaaa atacagtcac tgagggtact gatttacttg aaatcaaata
     4741 aatgtatttc cctgagtaaa gatggaattt tctttccaaa atcaaataat atagattaat
     4801 caaatatttg agaataacat tttatttaat cttttcatat atcaattgta tgaaaaatta
     4861 ttagaatttt aaaaaatgat aaaaattcaa aaagaaaaaa tagtagttat ttacttgtca
     4921 tgttactaaa taaaaaaata atttaaatat gatatatagt catttattta gtgatttcat
     4981 tctaaaataa agggagaaat gtctatgcta tacggtgcaa ttgaagcagg cggcacaaaa
     5041 tttgtgtgtg ccattggcga tgaagaaatg acgatcaagg aaagagtgag ctttccgaca
     5101 acgacacctg aggaaacaat gcctttagtc attgacttct tcaaacaata ccaagcagat
     5161 ttagcaggaa ttgggattgg ctcatttggg cctatcgata tccatcgcga ttccgccact
     5221 tatgggtaca ttacctcgac cccgaaa
//
LOCUS       DASHHB010000106         5196 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.112, whole genome shotgun sequence.
ACCESSION   DASHHB010000106 DASHHB010000000
VERSION     DASHHB010000106.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5196)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5196)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5196
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.112"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..312)
                     /locus_tag="U2K71_002710"
     CDS             complement(<1..312)
                     /locus_tag="U2K71_002710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM7316717.1"
                     /translation="MTYTHLTPNELVMIEAYFHQETPVAIVAKQLKRGRQTIYNVYNF
                     LKCGGTALEYFEQYKENKRRCGRTEIIFPAEEKEYIEKRSTEGWTPDVIIGRAERTFS
                     CS"
     gene            955..1518
                     /locus_tag="U2K71_002711"
     CDS             955..1518
                     /locus_tag="U2K71_002711"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7316718.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
     gene            complement(1797..2084)
                     /locus_tag="U2K71_002712"
     CDS             complement(1797..2084)
                     /locus_tag="U2K71_002712"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM7316719.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(2074..2403)
                     /locus_tag="U2K71_002713"
     CDS             complement(2074..2403)
                     /locus_tag="U2K71_002713"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM7316720.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            2708..3037
                     /locus_tag="U2K71_002714"
     CDS             2708..3037
                     /locus_tag="U2K71_002714"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7316721.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            complement(3324..3809)
                     /gene="vanZ"
                     /locus_tag="U2K71_002715"
     CDS             complement(3324..3809)
                     /gene="vanZ"
                     /locus_tag="U2K71_002715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEM7316722.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(3962..4873)
                     /locus_tag="U2K71_002716"
     CDS             complement(3962..4873)
                     /locus_tag="U2K71_002716"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEM7316723.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
BASE COUNT         1670 a          902 c          803 g         1821 t
ORIGIN      
        1 tgaacaagag aaggttcgct ctgcacggcc aataatgacg tctggggtcc aaccttcagt
       61 tgatcttttt tcaatgtatt ctttttcctc agcaggaaaa atgatttcgg ttctcccaca
      121 acgtcgctta ttttctttgt attgttcaaa gtattcaagt gctgttccac cacatttgag
      181 aaagttatag acattgtaaa ttgtttggcg tccacgttta agctgcttcg caacgatagc
      241 aaccggagtt tcttgatgaa aatatgcttc tatcattaca agctcgtttg gtgtaagatg
      301 ggtataagtc atttatgttc actctccttg tatgctttag cgggtattac aatttgagtg
      361 taacataaat ggctttttta tttgtctcgc ttaattatac aaacggcgaa gttaaaactt
      421 ttttagcaga ttttaaagtt tcgtcaaaac taatatattc tggtagctga gcattcaaat
      481 agctttcaga cttcagaaga aaattacgag catttttata gtccatctct aataaagtta
      541 ttcttttttt atccaataca ttcagctcaa ttccttataa tattctattc cttagtatct
      601 caacattatt tactttataa aacgaataaa ttttgtagtt ctaattcaaa tattgcatgt
      661 tatatctaat atgggagcat tttataattt tcattgtgat aaactcgtta ttaactttag
      721 gatacactag ttaggattaa attaggtatc tagaaattta cttaagattt ttttgtttat
      781 ttcgtataca cactcgtatc aaaaacaatc gattatgaga caaaatgaaa tgacctcttt
      841 tattaaataa aagaggtcat attttgtatc ataacttgtt gcattaacta tgtatctata
      901 actccctttt atgatacaat caagccagaa ttaaagtacg aaaggaagaa aagcatgagt
      961 ggatacattt atggttatgc ccgagtgagt acacgttcac aagaactaaa tcgtcaatta
     1021 gacttactca atgaacaaaa ttgtaatgaa atcctaactg agaaaatgac gggaaccaaa
     1081 gcggttcgtc cccaactaaa tcgtttaaaa gataaaattc gtccgggaga tacggtaatt
     1141 gtagaaagtt tttctcgatt gggtcgtagt acaaaggatt tgattgacct tgttacctat
     1201 tttgaagaac accatgttaa gctggtgagc ttgaaagaaa acttcgatac cagcacgcct
     1261 cacggacgct taatgatgac tgtcttccaa gcatttagtc agttcgaacg tgatttaatt
     1321 gttgaacgta ccaaagaagg tttgaaaagt gcacgtgcga gaggtcgtaa aggtggccgt
     1381 ccacgagtga accagaaaga tgttgatcga gcagtaaaat tgtataaaag tcaggtttat
     1441 agcgtaaaag aaattacgga aatgactggt attagcaagg caacactcta ccgctactta
     1501 aaagacaata gggaataatc tattttaatc tttaggaatc tataatattt atgggagaat
     1561 tagagtgatt tttttattat tactaaaaaa caatataaaa taacgcattt taagccctct
     1621 ttgtgcataa ggatgaagct tttaatttga aagaggctta aaatgcgttt taattttttc
     1681 tatttttcat aaatatatag gttagtggtt taaaattata gggtattaca actctcagct
     1741 gttaaaaatt atcttttaaa gtgtcgtcta tcctctcaag ttgaaacatt gggttcttac
     1801 ctaatctttt ttaattgttc ataaaaattt tcatgcgttc cacatagtaa gataggtatc
     1861 actgcattat ctttaatttc atatgcaacg cgatatgtcg tacctgcata cttgaatcca
     1921 atagcccaaa ttcctgataa atcacccgtt ttttgttctc ccgaatgagg acgaactgct
     1981 atctcatcat aaattatcgt taaaaattgt tcttttaatg ttttctcttt taattttttt
     2041 agatacttta ttacgtgttt agaaggtaga atctcataag ccaatttctg ctgccaactc
     2101 ctcttttgtc atcggtttag aatttgcaac agtatcttca gcaatagatt taaatgcaga
     2161 agttaacgca ttttttctat ttctaaattc ttttaagatc tcttgtttgc tataaccttc
     2221 tgaaagaata tcagccaaaa tatcttcact aaaatcaaaa aattcttttt taggctctac
     2281 aaactcataa aggataccat tctctacaag cttagcttct acttcaacac cattgggcac
     2341 atcgaattct ttagggaccg ttaacataat agaatttcct tgttttcttg tttttgtaat
     2401 catattattt cctccattat tttacatttc gtaattactt agtaattaca ttgtacgcta
     2461 tttttagtag aaatacaaaa taataaaact cctttagaac aattttcaaa ggagttttat
     2521 ttgattagga aattatgtag aataataata tgtttttaac ttggaatcaa aaacgcactt
     2581 gcaagtaagt ttacttcaat acatgaaatt gcttataata gtgtattgga attaaggtag
     2641 attgacaaaa gagtgatcaa ataggtatac ttcattcaaa taatcgtttg aatgtgaggt
     2701 gagatgaatg aaaaaagttt gccaagtgac tgtcgtacat aaagaaaaag taaatggaat
     2761 aaaaaataaa ttggatcaaa aagatttttc aaacttactt gtgttaggga aatgtttcag
     2821 cgatttttct agaattaaaa ttttctatgc tttagaaacc tataaggaaa tgtgcgtctg
     2881 tgatttagca gaaatactta ctgctagtgt cgctacaacg tcacaccatt tacgtttttt
     2941 aaaaaaacat ggaatggcaa aatcacgtca agatggaaaa gtagtttatt actctttagc
     3001 gaatgaagac atcctttccg ctattcgtgt tttttaaagt tatcagaaaa tctatctatg
     3061 aaatcttagt ttgggggtac cgtcaggaaa atgcggattt acaacgttaa ggaaatttat
     3121 taaagagacc aattcctcag cactaaaatt gataattggg ctttctttgt tactatagtg
     3181 ttaggtaagc tttttacttt atcaccggtt ttcccctcac ttcacaccta cgggcgagtt
     3241 tccccacatc ccgttttcca tctaatccaa ttcattcaat atctttatgt gtatgaaaga
     3301 tcagattgct tgatttagac atcttacacg taatttattc ttaaatgggt acggtaaacg
     3361 agcaataata cgagcaaaag tatacctaca aaaataataa ctctgtctaa ttttttttga
     3421 ttcatatgct tattgcttaa accatataat ttcagtccaa gaaagcctcc aacagtattt
     3481 gtaattacat ctgttatgtc tgtcgctcca atagcgaaga taaattgaat tatttcaaaa
     3541 gtaagactta aaaccagtac aaaagcaaac ttaggtaaaa atccgatttc tttaaaattg
     3601 acattcaaaa gcaagccaaa tggaataaag attataacat tatctatcat ctctctgaaa
     3661 ttcccagtag cagtaaatgg agtcaagtta agacttcttt gatgataatt aaatactgat
     3721 aaaatattgt attgtaattt gaataacact aaccagatta gtgtcactaa atataaagct
     3781 agcaatcctc tagataatat ttttcccaat ataatttcct ctaattcttt tgtttaagtt
     3841 gtatcattag tatagcaatg tcctagttaa cgtgggtttt aaattaaaaa gctaacgtta
     3901 ccccaaaagt gctagtacaa taatgctgtt cattaatttc gttggtttca ttccgccatc
     3961 cttacctcct tgaattagta tgtgttgatc cgggaaacac tgtcacaatt acaccgtcta
     4021 tattgtttcc tgatatctca taacgaagat tagtcggcac atgaatggtg gtatttttag
     4081 taacaggata ataaaagatc tcatattttt ccccatttac actaacagaa atggtttttt
     4141 cttcttttag gtaatccata tattcctcga gaacgaaatt cttttctttc ataatcgcac
     4201 tatgtggtaa accaacatag cgaatatgcc atggttcata ttgaattcct gttaactctg
     4261 ttttgtcctc tggataacgt aaaatgaacc cgtatttcca agcattttct tctatccact
     4321 ttccttcagg ggctcgttcc attttcgtca agcttgatcc tacatctagt gataaacctg
     4381 aattatgctc actataacct gctggtaagg catactcagc ccccatttct tggtaaagca
     4441 cactttgctc atcaaagtct cgatagccac tattaataat aaaatgacta acgccaccct
     4501 ttacagcatc attgaccatc tctgaaaatt tttgtgctat ttcttttgac atataaatat
     4561 tactatcaag caacccgtat ccatttatta attcgtcatg tttagataaa ttcacgatat
     4621 ctgacttcac actttcttgg cgaacaggat atttactatt gattaatagc agatttcctt
     4681 gataaacctg ttcttctgta attgttttct cttgggtatt ttcagaagtc ccactatttt
     4741 ctaaatgttc tttgggattt tgatcataat tttgaaattc gactttttcc tgagaaaaca
     4801 gtgcttcatt aacgtagtca taacctaagt atattaagaa taacaataac aataaaaaaa
     4861 acaacttctt cattttcagt ctcctcctta actatatagt tggaagtatg aaatatttct
     4921 agaatcttcc ccttaaacgg cacaatctcg tttgagattg tgccgttttg ccccgtaaat
     4981 tacagtcaat tatttcgccc tactggattt actattatca ccaatgtaga tgaattttca
     5041 taagtgatac ccatgctgaa tttaacgagg ctgaactgcc tgttgacaat gggcggtgtg
     5101 aaataccacc cctctatgcg atcagtctta ttatcaggct atcttgccgc agcgcacgca
     5161 gataatgacc gcaatatcgc tctgccttac atcttc
//
LOCUS       DASHHB010000107         5025 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.113, whole genome shotgun sequence.
ACCESSION   DASHHB010000107 DASHHB010000000
VERSION     DASHHB010000107.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5025)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5025)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5025
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.113"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            283..522
                     /locus_tag="U2K71_002717"
     CDS             283..522
                     /locus_tag="U2K71_002717"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316724.1"
                     /translation="MKRILFISLSNLLLLTACGTSASKNIPSDTQISNSIDSTISTNE
                     KNSPVETSDIKVSTSAKNRVKKLSARLLYFKQTLN"
     gene            complement(884..1144)
                     /locus_tag="U2K71_002718"
     CDS             complement(884..1144)
                     /locus_tag="U2K71_002718"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316725.1"
                     /translation="MNNETIILGKKYICQPIGLKQKVVGEVISKLENCVVICVDEYHD
                     VDRNEILEKCGKVVAKYTHVYELETTEYLYNYSNKYEHTLVS"
     gene            complement(1329..1826)
                     /locus_tag="U2K71_002719"
     CDS             complement(1329..1826)
                     /locus_tag="U2K71_002719"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HEM7316726.1"
                     /translation="MWTEKQKKDAYEILLLQQQGLLEAEDNWLANLANSSALLNETLP
                     ETVFAGYYLFDGNELILGPFQGRVSCTRIKMGKGVCGESAEKQETLIVDDVKTHANYI
                     SCDSAAQSEIVVPMVKEGNLIGVLDIDCGVTKGYDEIDQAFLEKYVDLLLANFNINAF
                     SFINN"
     gene            complement(1956..2666)
                     /gene="budA"
                     /locus_tag="U2K71_002720"
     CDS             complement(1956..2666)
                     /gene="budA"
                     /locus_tag="U2K71_002720"
                     /EC_number="4.1.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate decarboxylase"
                     /protein_id="HEM7316727.1"
                     /translation="MTEKILYQHGTLGALMAGLMDGTETIAHILEKGTLGLGTLHGLD
                     GEVIFLDGVAYQGRSDGSVAQLDGSELTPYAAITDFTPDTSFSVSKTADGEQLKKDIL
                     VREAGENLFLAVKITGLFKNMHIRIMPKQEKPYRRLAKISESQPEFQQSNVQGTLVGF
                     FTPELFQGVAAAGFHLHFIDDTHTFGGHVMDFEVAEGKVEISRISSLVQHFPVDDPTF
                     VHETIDYADLAAEIQQAE"
     gene            complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="U2K71_002721"
     CDS             complement(2679..4343)
                     /gene="alsS"
                     /locus_tag="U2K71_002721"
                     /EC_number="2.2.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetolactate synthase AlsS"
                     /protein_id="HEM7316728.1"
                     /translation="MDEKMKQGSKSVVESLINHHVDYVFGIPGAKIDGVFNELEDQGP
                     ELIVTRHEQNAAFMAQAIGRITGEPGVVIATSGPGASNLATGLVTATAEGDPVLAIAG
                     QVKRSDLLKLTHQSMDNAALFQPITKYSAEIQDPETISEVIANAYRMAKSSKKGASFI
                     SIPQDVVDAPVQGNVIKPLSDPKLGSASADDIRYLAERIREAKLPVLLVGMRGSSEKE
                     TLAIRQLVGKTALPVVETFQAAGVISRELEAHFFGRVGLFRNQPGDMLLKRSDLVIAI
                     GYDPIEYEARNWNAEKDARIIVIDEAPAEIDPFMQPERELIGDISATLDLLTGSLEPQ
                     QVSEDAKEYLASLQAELTERDIVQSKGEAGILHPLEVINTLQSKVTDDMTVTVDVGSH
                     YIWMARHFRSYEPRHLLFSNGMQTLGVALPWAISAALVRPNTQIVSVSGDGGFLFSAQ
                     DLETAVRKKLNIVHLIWNDGHYNMVEFQEKMKYQRASGVDFGPVDFVKYAEAFGAKGI
                     RATSVEELEKALEEGFATEGPVIIDIPIDYRDNEKLGETILPDQFY"
     gene            complement(4548..5015)
                     /locus_tag="U2K71_002722"
     CDS             complement(4548..5015)
                     /locus_tag="U2K71_002722"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316729.1"
                     /translation="MIGMTEELAKEKSFSPVRLFIKTFRQFWLKGFFYWLFAWIVSVI
                     MIFDCFFFIRFSYGKWLIPLFVLLACLSVSFSINYWYFQVRNPASKPNQVLRIAFYYT
                     LKKWYVSLLDFLLLTSLFLFFFVKPQWCILLGPSIVFGLIYFNNRKLMRTMDL"
BASE COUNT         1529 a         1043 c          771 g         1682 t
ORIGIN      
        1 ctatcctcct agcaactatt taaataccca aaaaaattac ctctagataa tatcatacct
       61 acgttttcac ttataaattt tttcataaaa aaagcgttta gaggcataat cgttaataca
      121 gatggtaacc aatgtatctg gtttgaactg ggatatcacc ctctccatat atgtattact
      181 aagactaata gatactataa aagacttgat agttttgcat tgattttgcc taatactgag
      241 cataggtgta tgctaaacga tactataagg agtgtgtaca taatgaaaag aattctgttt
      301 atcagtttat caaatttatt gttacttact gcttgtggga cttctgcatc aaagaacata
      361 ccttcagata cgcaaatttc gaatagtatc gattcaacaa tttctacaaa tgagaaaaat
      421 tctccggtag aaacttccga tataaaagtt tctacctctg caaaaaacag ggtaaagaaa
      481 ctgtcagcac gtctccttta tttcaaacaa actctgaatt agcttcttca tcttcttcag
      541 caaataatga tgatagtttc gcgtttactc ctaactaaat gcaaccttta caaatgcttt
      601 acgataacaa gcctgagcta aatgatcctg atatcagttt tgcatttttt gaaatgattg
      661 gatcggatga tctttttaaa actactagtt tgtctatccg aaaataggac agttctggca
      721 gtataggttt ttatcgagtt tctccaccag gcctcgttac agagacagat tctctaggcg
      781 aaccttttga ataaatcaaa aatcatgaac aatcactgcc aactaaataa agtgccttca
      841 aaagcttggt cttctcgctt ttgaaggcac tttatttaaa tctttatgaa actaaagtat
      901 gttcatattt gttgctatag ttatatagat attctgttgt ttccaactca taaacgtgag
      961 tatattttgc cactactttt ccgcattttt ctaatatttc attgcgatcg acgtcatgat
     1021 attcgtctac gcagatcaca acacaatttt ctaatttact gattacttct ccaaccactt
     1081 tttgtttcaa gccaataggt tggcaaatat atttttttcc taaaatgata gtttcgttat
     1141 tcatatttca tccatcctta atgtttttcc attatttaaa atgataatac cattaaaata
     1201 tgattatgaa aatcaataac acttgtgatt ttataaaaaa ataaaaaaac cgaaaaacag
     1261 aaatgcccac tgtctttccg gctcttttta ttcttgattt ctcttctttt ttgtttcaat
     1321 agacgatttt agttattaat aaatgaaaaa gcatttatat taaaatttgc taataaaaga
     1381 tctacatatt tttctagaaa tgcttgatcg atttcatcat accctttcgt tactccgcaa
     1441 tcgatatcta aaacaccgat taaattacct tcttttacca ttggcactac gatttctgat
     1501 tgtgcggctg aatcgcagga aatgtaattt gcatgggttt tgacatcatc aacgatcaat
     1561 gtttcttgtt tttcagctga ttctccacaa actccttttc ccatcttgat acgtgtgcaa
     1621 gaaactcgcc cttggaacgg tcctaaaatc aattcattac catcgaataa ataatatcct
     1681 gcaaagacag tctcaggcaa agtctcattc aacaatgcag atgaatttgc taaatttgct
     1741 aaccagttat cttctgcttc taataatcct tgttgctgta ataataaaat ttcgtatgca
     1801 tcttttttct gtttttctgt ccacatgata aaaaccgctc ctttttgatc tatggagaag
     1861 attatagctc agtttttttg taaacgctag gtttcctcct caaaaaaacg agatacgtca
     1921 ggttaatcac cttgaacgca tctcgctgtc tttttctatt ctgcttgttg gatttctgct
     1981 gctaaatcag cataatcaat cgtttcgtga acaaaagtcg gatcatcaac tggaaaatgc
     2041 tgtacaaggg acgagatgcg gctaatctca acttttcctt cagccacttc aaaatccatt
     2101 acatgtccgc caaatgtatg tgtatcatcg ataaaatgca agtgaaatcc cgctgcagca
     2161 actccttgga ataattcagg cgtaaagaac ccgactaatg ttccttggac attgctttgt
     2221 tggaattctg gttgagactc tgaaattttt gctagtcttc tgtaaggttt ttcttgcttc
     2281 ggcatgatac ggatgtgcat atttttgaat aatccagtta tcttcactgc caaaaatagg
     2341 ttttccccgg cttctcttac taatatgtct tttttcaatt gttctccatc tgcagttttt
     2401 gagacagaaa aactagtatc tggcgtaaaa tctgtaattg ctgcataggg ggtaagttca
     2461 cttccatcta attgggcaac tgagccatct gatcttcctt gataagctac gccatctaaa
     2521 aagatgactt caccatcaag cccatgcaat gttcccaatc ccagagttcc tttttctaat
     2581 atatgagcaa tcgtttctgt tccatccatc aagccagcca tcaaagctcc taatgtcccg
     2641 tgctggtata agattttttc agtcatgtcg acacttcctt aatagaattg atctggtaag
     2701 attgtttctc ccaatttctc attatcacgg taatcaatcg gaatatcaat aattacgggt
     2761 ccttctgtcg caaatccttc ttctaaagct ttttctaatt cctctacact tgtagctcgg
     2821 attcctttag cgccaaaagc ttctgcatat ttcacaaaat caactggacc aaagtctaca
     2881 cctgacgccc gctgatattt cattttttct tggaactcta ccatattgta gtgtccatca
     2941 ttccaaatca aatgaacgat attcaatttt ttacgtaccg ccgtttctaa atcctgggca
     3001 gagaataaaa atcccccatc gccggaaact gatacgattt gtgtatttgg acgcaccaat
     3061 gcagcagaaa tcgcccatgg caatgcaaca ccaagcgttt gcataccatt actgaataac
     3121 aaatgacgag gttcataact tctaaagtgg cgtgccatcc aaatataatg actacctaca
     3181 tctacggtaa cagtcatatc atctgttact tttgactgaa gcgtgttaat aacttctaaa
     3241 ggatgcaaga tacctgcttc tccttttgat tgaacaatgt cccgctccgt taattctgct
     3301 tgaagagatg ctaaatattc ttttgcatct tctgacactt gttgaggctc taatgaacct
     3361 gtcaataaat caagagtggc tgagatatca ccgatcaatt ctctttcagg ctgcataaaa
     3421 ggatcgattt cagctggcgc ttcatcgatc acaatgatcc gcgcatcttt ttccgcattc
     3481 cagtttcttg cttcatattc gattggatca tagccaatag caataactag atcacttcgt
     3541 ttcaaaagca tatctcctgg ttggtttcta aataaaccca cacgaccaaa gaaatgcgct
     3601 tctaattcac gtgagatcac tccagctgct tggaatgtct ctacaacagg caatgctgtc
     3661 tttccaacta attgcctgat ggctaacgtt tctttttcac ttgatcctcg catacctact
     3721 aatagtaccg gtagttttgc ttctctaatg cgttcagcta ggtaacggat atcatctgca
     3781 gaagcagaac ctaatttagg atcgctcaat ggcttgatga cattcccttg gacaggtgca
     3841 tccactacat cttgcgggat actgataaag ctcgcgcctt tttttgaact cttagccata
     3901 cgataagcat tggcaatcac ttctgaaatt gtttctggat cttggatttc tgcactgtat
     3961 tttgtgattg gctggaacag tgctgcatta tccatgcttt gatgggtcaa cttcaataga
     4021 tcgcttcgtt ttacttgtcc ggcaattgct aaaactgggt caccttctgc tgtcgctgtt
     4081 accaaacctg tagctaagtt actagcacca ggaccacttg ttgcgataac tactcctggc
     4141 tctcctgtaa tacgtccgat tgcttgtgcc ataaatgcag cattttgttc gtgacgagtc
     4201 acgattaatt caggaccttg atcctctaat tcattaaata caccatcgat ttttgcacct
     4261 ggtataccga atacataatc aacatgatga ttaatcaaac tttctactac agatttcgaa
     4321 ccttgtttca ttttttcgtc cattgaacat cgtccttttt atgctttatt tttaattatt
     4381 agttagaaat aatcacaaat aattgataca gtttctaaac ctgtttcatt caaaagtata
     4441 gcacgtttta tttcatttgt ttagccatat gtttcaaaaa atgaaaaaga atgacagttt
     4501 ttacataaaa actgtcattc ttaattgaaa gtttcaattt ataaagtcta cagatccata
     4561 gttctcatca atttacgatt gttgaagtaa atcaaaccaa atacgataga aggacctagt
     4621 agaatacacc attggggctt tacgaaaaag aagagaaaca aacttgtcaa tagtaaaaaa
     4681 tctagtaaac tcacatacca ttttttcaac gtatagtaga acgctatccg tagaacttga
     4741 tttggtttcg acgctggatt tctcacctga aaataccagt agttgatgct aaaggaaact
     4801 gataaacaag caagtaaaac aaacaacgga atcaaccatt ttccataaga aaaacgtata
     4861 aaaaagaaac aatcaaaaat cattatcaca ctaactatcc aagcaaatag ccaataaaaa
     4921 aatcccttta gccaaaattg gcgaaacgtc tttatgaaca aacgtactgg tgaaaaactt
     4981 ttttcttttg ctaattcttc tgtcatacct atcatcacga tgcaa
//
LOCUS       DASHHB010000108         4926 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.114, whole genome shotgun sequence.
ACCESSION   DASHHB010000108 DASHHB010000000
VERSION     DASHHB010000108.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4926)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4926)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4926
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.114"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            85..972
                     /locus_tag="U2K71_002723"
     CDS             85..972
                     /locus_tag="U2K71_002723"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016627954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucosaminidase domain-containing protein"
                     /protein_id="HEM7316730.1"
                     /translation="MQELKTRSARHHRPKRKMVINKKAIAPLIGTSLLVGPMMVTAVP
                     QHVHADEQGSQTVADPQAFIDQIGWSASEVAASNDLYASVMIAQALLESAYGTSGLAS
                     APNYNLFGVKGSYNGQVVYMATKEYVNGQWVTMNEPFRKYSSYWESFQDHANVLRSTS
                     FSTGDYHYAGVWKSNTTSFYDATAYLTGRYATDPNYGAKLNSLINTYNLTRFDTPSTG
                     SATTTTSTTTTTTTTTSTSSSSTTDVNTNNGQTYTVVSGDTLWDIAQKFGMSVDDLMA
                     KNGMSMSSYSLLVGQELNV"
     gene            1112..2038
                     /gene="cysK"
                     /locus_tag="U2K71_002724"
     CDS             1112..2038
                     /gene="cysK"
                     /locus_tag="U2K71_002724"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HEM7316731.1"
                     /translation="MTAIYNSVTELIGKTPIVKLNKIVPEDSADVFVKLEFFNPGGSV
                     KDRIALSMIEKAEHDGLLKPGDTIIEPTSGNTGIGLSMVGVAKGYKVIIVMPETMSIE
                     RRLLMKGYGAELILTPGADGISGSIREAERLAKENGYFLPLQFENEANPLVHEKTTGP
                     EIHQAFGVNGLDAFVAGIGTGGTITGAGRELKRVYPKIELIGVEPAESAILEGKEAGP
                     HKIQGIGTGFVPKTLDTSVYDKVLSISGDEAMETAREVGRKEGILVGISSGAAIAAAL
                     KVAKELGKGKKVLAVVPDNGERYLSTALYQEI"
     gene            2274..2720
                     /locus_tag="U2K71_002725"
     CDS             2274..2720
                     /locus_tag="U2K71_002725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor"
                     /protein_id="HEM7316732.1"
                     /translation="MENKLVEEVIEKLKKENIRITPQRYAVLEYLIEHHSHPTADEIY
                     KALEHRFPNMSVATVYNNLRLFTSIGFVQEMKYGDASSRFDFSSKRHYHVICENCGKI
                     VDFHYPGLEDIEMAASRLTDFEINEHRLELYGLCPDCKESAKKAQM"
     gene            2993..4345
                     /locus_tag="U2K71_002726"
     CDS             2993..4345
                     /locus_tag="U2K71_002726"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH oxidase"
                     /protein_id="HEM7316733.1"
                     /translation="MKVVVIGCTHAGTAAVKSILKNHPEAEVTVYERNDNISFLSCGI
                     ALYVGGVVKDPAGLFYSNPEELSSLGATVKMEHDVTDVDTENKTVTAKDLKTGETETV
                     SYDKLVMTTGSWPIIPPISGIDAKNVLLCKNYNQANEIIAQAKEAKRVVIVGGGYIGI
                     ELVEAFVESGKQVTLIDGLDRILNKYLDKPFTDILEKELTDRGVTLALGENVTEFITD
                     EEGKVKQVATPTDTFDTDMVILCVGFRPNTKLIEGKVDTLPNGAIKVNEYMQTSNPDI
                     FAAGDSAVVNYNPSGTQNYIPLATNAVRQGLLVGNNLTDHKMAYRGTQGTSGLYLFGW
                     TIGSTGVTKESATLNDLEVQATVFEDNYRPEFMPTTEKVMMELVYEKGTNRIVGAQFM
                     SKYDITQSANTMSLAVQNKMTVEDLALSDFFFQPHFDRPWNYLNLLAQAALGEIETAQ
                     "
     gene            4516..4803
                     /locus_tag="U2K71_002727"
     CDS             4516..4803
                     /locus_tag="U2K71_002727"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316734.1"
                     /translation="MNKEYFVQQDAFLEDYKSASKKLTINIDELADQVLSQLNETQEE
                     YYTMPANDAKDNVSHRFPFSRRIYSDENDGTLNTEYTYEGQPYVLETDDDL"
BASE COUNT         1695 a          813 c         1030 g         1388 t
ORIGIN      
        1 aattaaaagt ataatttaat taattaagtt tgttttaaga agagaacttt ttattaagtt
       61 tttttatttt gaggaggaat ttgaatgcaa gagctgaaga cccgcagtgc ccggcaccat
      121 agacctaaaa gaaaaatggt gatcaataaa aaagcgattg ctcctttaat tggaacgagt
      181 cttttagttg ggccgatgat ggttacagcg gtaccgcaac atgttcatgc agatgaacaa
      241 ggatctcaaa cagtcgctga tcctcaagcg tttatcgatc aaatcggctg gtctgcgtca
      301 gaagttgctg caagtaatga cttgtatgct tcagtcatga tcgctcaagc gttgttggaa
      361 agtgcatatg gcacttccgg attagctagt gcgccaaatt ataatctatt cggtgtgaaa
      421 ggaagctata acggacaggt cgtttatatg gcaaccaaag aatatgtaaa cggacaatgg
      481 gtaacgatga atgagccatt ccgaaaatat tcttcttatt gggaatcctt ccaagaccat
      541 gcgaatgttc ttcgttcaac cagtttttcc acgggcgatt atcattatgc tggcgtgtgg
      601 aaaagcaata caacaagttt ttatgatgca acagcgtact tgacaggtcg ttatgctacc
      661 gatccaaatt atggggccaa attgaactca ttgattaata cttataattt aacccgtttt
      721 gatacgccat caacaggttc ggctacaaca accacaagta caacaacaac gactactacg
      781 acgacttcaa cgtcatcatc ttcaacaaca gatgtaaata caaataatgg tcagacatat
      841 acagtcgtgt caggtgatac gctatgggat attgcacaaa aatttggcat gtctgttgat
      901 gatctaatgg cgaaaaatgg catgtctatg tccagttatt ctttattagt tggtcaagaa
      961 ctaaacgttt gatttagata aaaaggcaaa gaaattacca tatatggtat atctttgtct
     1021 tttttttgct agtcaaagac gaatcactcg ccataaatga acagattgta ttatgataag
     1081 taagaagagc aaagacaagg aggaattgaa gatgacagct atctataact cagtcaccga
     1141 attgattggc aaaacgccaa tcgtcaaatt aaataaaatc gtaccagaag attcagcaga
     1201 tgtttttgta aagcttgagt tttttaatcc aggaggaagt gtaaaagatc gtattgcttt
     1261 aagtatgata gaaaaagcag aacacgatgg tctactgaaa ccaggtgata ctatcattga
     1321 acctacttct ggaaatacag gaatcggatt gtcgatggta ggagttgcaa aaggatataa
     1381 agtaatcatc gtgatgcctg agacgatgag tatcgaaaga cgtttattga tgaaagggta
     1441 cggcgcagag ttgattttga ctcctggtgc ggatggtatt tctggatcaa tcagagaagc
     1501 agaacgtttg gcaaaagaaa acggctactt tttaccatta caatttgaaa atgaagcaaa
     1561 tcctcttgtg catgagaaga cgactggtcc tgagatacat caagcatttg gagtaaatgg
     1621 cttggacgcg tttgttgcag gtatcggtac aggaggaacg atcactggcg ctggccgaga
     1681 attaaaacgt gtgtatccga aaatcgaatt aatcggggta gaaccagcag aatctgctat
     1741 tttagaaggg aaagaagcgg gacctcacaa aatccaagga atcggtacag gttttgttcc
     1801 taaaacactg gatacttctg tgtatgataa agtcctttct attagtggag acgaagcgat
     1861 ggaaacagct agagaagttg ggcgaaaaga agggatttta gtaggcatct catctggcgc
     1921 tgcaattgct gccgcgctaa aagtcgcaaa agaattagga aaaggcaaga aggtattagc
     1981 tgtggttccc gacaatggtg aacgttattt atctacagcc ctttaccaag agatataagg
     2041 cacttggttt ttctccagat aggtttatgt aaaaaacttt taaagatgaa aagaacacac
     2101 aaaaatttat gatgagaaaa attatttgaa tagagacgta gaatagtgaa cgcgactgtt
     2161 ttacgtcttt tttcattaaa atacaataaa attgaatttt cttttcatgt taagtggatt
     2221 ttaaaatgta aaaatagtat gataagacga gaataacaac gtggggtgtt tacatggaaa
     2281 ataagttagt cgaagaggtt atcgaaaagt taaaaaaaga gaacattcga atcacacctc
     2341 aacggtacgc ggttttagaa tatttgattg aacatcattc ccatccaacg gctgatgaga
     2401 tttataaagc acttgaacat cggtttccaa atatgagtgt tgcgactgtt tacaataacc
     2461 ttcgcttatt tacttcgatt ggatttgttc aagaaatgaa gtacggcgat gcttctagcc
     2521 gatttgattt cagttcaaag cgtcattatc atgtaatttg tgaaaactgc ggtaagattg
     2581 ttgattttca ttacccaggg cttgaagata tagaaatggc tgcaagcaga ctgactgatt
     2641 ttgagattaa tgaacatcgg ttggaattgt atggactctg tccggattgt aaagagtcag
     2701 caaaaaaggc gcaaatgtaa aaacagtttc tacaagtgtt atacctaaaa aaagctagct
     2761 gtgtaaaaaa aacgctagct tttttatttt tttgtctttt attcacaatc ttagatataa
     2821 aagtgaatat tattaaaata agaatcttgt agatattggg ttcttatgct tgaaaaggaa
     2881 tgaaactaca aacaaacctt tgtgaattca caagcattaa actgttgtat ccgttttatt
     2941 tctgttatag tagttttgtc gaaggacaaa atgttaatcg gagggaccat aaatgaaagt
     3001 agtagtaatt ggctgtactc atgcaggtac tgcagctgta aaaagtattt taaaaaatca
     3061 tccagaagca gaagtgacag tatatgaaag aaatgacaat atttctttct tatcttgcgg
     3121 tatcgcactt tatgtcggcg gagtagtaaa ggacccagca ggcttgttct actcaaatcc
     3181 tgaagaatta tcttcattag gagcaacagt taaaatggaa cacgatgtaa cagacgtcga
     3241 tacagaaaat aaaacagtaa cggcaaaaga tctgaaaaca ggagaaacag aaacggtttc
     3301 ttatgataaa ttagtgatga caactggttc atggcctatt atcccaccga ttagtggaat
     3361 cgatgcgaaa aatgtactcc tatgcaaaaa ctacaatcaa gcaaatgaaa ttattgcaca
     3421 agcgaaagaa gcaaaacgtg tagtaattgt tggtggtgga tatatcggta ttgaattagt
     3481 agaagctttt gtcgaatctg gaaaacaagt gacattgatt gatggattag atcgcatctt
     3541 gaataaatat ctagataaac cattcacaga tatcttagaa aaagaattga cagaccgcgg
     3601 tgttactctt gcgctaggcg aaaatgttac tgaatttatc acagatgaag aaggaaaagt
     3661 gaaacaagta gctacaccaa cagatacatt tgatacagat atggtcattt tatgtgtggg
     3721 cttccgtcca aatacaaaac tgatcgaagg aaaagtagat acactgccaa acggggcaat
     3781 caaagtgaat gaatacatgc agacaagtaa tccagacatt tttgcagctg gagactctgc
     3841 agtagtcaac tacaatccaa gtggcacgca aaactatatt cctttggcaa caaatgccgt
     3901 tcgtcaaggt ttgcttgtgg gaaataactt gacagatcat aaaatggcgt atcgtggtac
     3961 tcaaggaact tcaggacttt acctatttgg ctggacgatc ggttcgacag gtgtgaccaa
     4021 agaaagcgca actttgaacg atctagaagt ccaagcgact gtatttgaag ataattatcg
     4081 tccagagttc atgccaacta ctgaaaaagt aatgatggag ctagtctatg aaaaaggaac
     4141 gaatcgtatc gtaggtgctc agttcatgtc caaatacgac attacacaat cagcaaatac
     4201 catgtcttta gctgttcaaa ataaaatgac tgtagaagat ctcgcacttt cagatttctt
     4261 cttccaacca cattttgacc gcccatggaa ttacttgaac ctacttgcac aagcagcgct
     4321 aggcgaaatc gaaactgcac aataaagaaa aagggttgtg acacaatttt gtcacgaccc
     4381 tctgtctttt tttgtgaaaa gtacaattag ttgtctttct tcgaattttt tgtcacaata
     4441 agcagcaaag ataaagctgt tacataaaag aacaagcagc tttatttgat tttgaaaaga
     4501 aaggtgagaa agaagatgaa taaagaatat tttgtccaac aagatgcatt tttagaagat
     4561 tataaaagtg cttcgaagaa attaacgatc aatattgatg agttggcaga tcaagtactc
     4621 tctcagttga atgaaacgca agaggaatat tacacgatgc cagcaaatga cgccaaagat
     4681 aatgtttctc atcgttttcc tttcagtaga cgcatctact cagatgaaaa tgacggcaca
     4741 ttgaatacag agtacacata tgaaggtcag ccatatgtgc tagaaactga tgatgatctt
     4801 taaagacagt ttttgaaaag gccgctcatc cttaagtgat gacaaaaaat catcacaatt
     4861 accgatgact gtctctttac tatcttttaa tcagaaaaag gggttatgac aagctttgtc
     4921 aacccc
//
LOCUS       DASHHB010000109         4810 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.116, whole genome shotgun sequence.
ACCESSION   DASHHB010000109 DASHHB010000000
VERSION     DASHHB010000109.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4810)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4810)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4810
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.116"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(92..1177)
                     /gene="xerS"
                     /locus_tag="U2K71_002728"
     CDS             complement(92..1177)
                     /gene="xerS"
                     /locus_tag="U2K71_002728"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177643.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerS"
                     /protein_id="HEM7316735.1"
                     /translation="MKNTEIYQKIDTIILHMPDYIKDFVQDREDKDQSPRTTLEYLKN
                     YKLFYEWLLSESIVPDTSITSIRQLTAYDLNLYKSHLKRRTKENVKKETTKTKLKDNN
                     NLGLSTSTINRNITALKVLFKYLSKSSNNPLGKPYLENNPMDQVATITDKTTLAARAN
                     AIEKKLFLDEDTQNYLDYIANEYKKTLSKRALIYYQRDVERDLAINALILGSGLRLSE
                     VVNINLEDLSLEKNNVVVTRKGNKRDAVNIAAFAMEYLANYLAIRKERYKVTENEKAL
                     FLAIYQGEAKRISGIAIERMVAKYSKGFRVQVSPHKLRHTLATRLYQQTNSLVLTAQQ
                     LGHTSTNTTTLYTHIDNAATIDALNAL"
     gene            complement(3253..3771)
                     /locus_tag="U2K71_002729"
     CDS             complement(3253..3771)
                     /locus_tag="U2K71_002729"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013852041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HEM7316736.1"
                     /translation="MRTYKNKEELKKEINRTYTKYILEFETIPEELKDKRVEEVDRTP
                     AENLAYQVGWTNLILKWENDERNGLSVKTPSDQFKWNQLGELYKWFTNTYAHLPLKEL
                     EEILNRNIDDINMMIDSMSDEDLFTAHKRKWADEATKTAVWEVYKFIHVNTVAPFGTF
                     RTKIRKWKRLAL"
BASE COUNT         1581 a          786 c          729 g         1714 t
ORIGIN      
        1 atataaaaat gataatgaag cttgccttta aagttgggct tttatctaat tttagagaca
       61 ggcttcatta ttatactatt ttttactaca tttacaaagc gttcaaagca tcaattgtag
      121 ctgcattgtc tatatgagta tataaagttg ttgtatttgt acttgtatgt cccaattgtt
      181 gggcagttaa taccaatgaa tttgtctgtt gatagagtct agtagccaaa gtatgacgta
      241 atttatgtgg actgacttgg actctgaatc cttttgaata ttttgctacc atccgttcga
      301 ttgcaatacc ggatatacgc ttggcttctc cttgataaat tgctagaaat aaagcttttt
      361 cgttttctgt tactttgtat cgttctttcc gaatagctag ataattagct aaatattcca
      421 ttgcaaatgc tgctatattt actgcgtctc tcttatttcc ttttctagtt actaccacat
      481 tattcttttc taaagatagg tcctctaaat taatatttac aacctcggag agtcttaatc
      541 cagatcctaa aatcaatgca ttgatcgcca ggtctctttc tacatctcgt tgataataga
      601 tcaaagctcg tttgctcaaa gtttttttat attcattggc aatataatct agataatttt
      661 gtgtatcttc gtctagaaat aattttttct cgattgcgtt tgcacgggca gctaacgtag
      721 ttttatctgt gatagttgct acttgatcca taggattatt ttctaagtat ggttttccta
      781 aaggattatt ggaacttttc gaaagatatt taaatagaac ctttaatgct gtgatatttc
      841 tattgatagt agaagtagat agacctaagt tattattatc tttgagctta gtttttgttg
      901 tctctttttt tacattttcc tttgtgcgac gtttcaaatg agatttgtac aagtttagat
      961 cataggcagt taactgtctt atcgaggtga ttgaagtatc gggaacgata gactcactca
     1021 gaagccattc ataaaagagc ttataatttt ttagatattc taaagtagtt cgaggtgatt
     1081 gatctttatc ctcacgatct tgaacaaaat cttttatata atcgggcata tgaagtatga
     1141 ttgtatctat tttttgatat atttctgtat ttttcatgtt attcatcctt tcgataagta
     1201 ggggagagga gtatctctat aattggacta taccaattat agagtgtttt gtcaattggt
     1261 atactctgaa atttattaat ttatttcaaa aaagcagata aataggtata gatttaataa
     1321 tttaaaataa atggtataca tgaataggaa aggaaacaga aggaggcaat cttaattttt
     1381 taaattgtct aaattatata gaaagaacaa tctttctata taattagaca taagagagac
     1441 tacggttgat ttgatattaa caagcgtata tgaatgtata tacaaataaa aaaggggagg
     1501 gagaagttaa agaaaattca gagaagtggg gcactcagta gtattaacaa tttaaaagga
     1561 atttcttaaa gaaaatgggt tgcatcctgg agatacaatt tttttagata aagaaaaaat
     1621 tcaagcaaca attcaaaaaa atcttaactg ctgagattga tgtagcaata tctcaatcat
     1681 caacaaagta cgatcctgcc tttcaaaagc ttattgataa atgtttattc attataaaaa
     1741 taaaagaaaa atttatgtca gcttttttca tcaaagtggt aatgataatt cgtctattaa
     1801 ttgtattttt taggaaatat ggtataattg ttttaacgaa aaggtaattc atgatacacc
     1861 aaaaaccact agctggcggg ctagtggttt tcttttacgt tctattcaga ttaacgggta
     1921 ggcttactgt cttttttggc aataattttg caatcaagcc atttacagat gtaatgcgcg
     1981 gatacttctg ctaagattgc aaaaaacata tcgaaaaggt aattcataat acaacctcct
     2041 ccccttgcca taataggccg gggtagcaga cagcataaat attataccat aactgaaaat
     2101 ttcattttag tacatttcaa tatattgatg aaccagcaca ataaaacatg ccatgactaa
     2161 cagaaaaaaa cttattaaac cagatataac ttgtaaacat ccaactatat agaaaacgta
     2221 aattttctat atagttggat taagataacg actatttatt ttatcatatt tttactatac
     2281 cctatacttt tacttggtat atttatgaat aatcgcaaat ttacccctat actactagtt
     2341 tgtaagttag attaataaaa atactaaagt tatttgttct tttattaaat acacctacaa
     2401 cttataaatt gatggtattt agataacacg aaagaataaa aataacagct gatagtaaag
     2461 tttgttgacg attgctactc aatcgaaagt gagttagtgc gtatgaactt gcttctgaaa
     2521 acttatgaaa tgaaagacct tttacatctt gcagaaatcg ttactgggat taaactaagc
     2581 aataaaaact aatgttcccc tcattctttg aacttctact atttgattta tttattctga
     2641 taaatgacga tttcaccttc aggatcttca aatcccgtat agaatttatt taagatctct
     2701 tcagtatcag taataggata ggcgataggc cacataccct ttgatggtta gaataaactg
     2761 ctcaaaatct gtatcgtatg ttggctttag catattaaca aaggctatct ccaaattata
     2821 aaccataagc gttgaatcgt tcttaacaat ttcaattctt cctaacccaa agtcagaaga
     2881 tataattacc ggacttgcct tacccttcat gtgggttgtt gagattccat atttaaaggg
     2941 aattgtcggt actatcggcg gtctgaaaaa catcccaaaa ataaaaaaat ctttcccctg
     3001 ctagtctcct aagtcccttt gtagcggata tggcaccata atctcatttt atcgaagtga
     3061 gtcatggttt atctgttggc caaccaaaat tgatacaatc taacgatgca ccctctacgg
     3121 cattctaggg gagtgcactt gcattcaccc aataaaaacc ggcagttagc aaagaactta
     3181 tgtagtcgta tgttcttact gttaacatac aagaggtctc ctttccacaa ttgcttagca
     3241 gtaaacggga atttacaaag ctaatctctt ccattttctt atctttgttc taaaagtccc
     3301 aaatggagca actgtattta catgaataaa tttatatact tcccatacag ccgttttggt
     3361 tgcctcgtct gcccattttc ttttgtgtgc agtgaataga tcttcatcac tcattgaatc
     3421 aatcatcatg ttaatatcat caatattacg attgagaatt tcctctaatt ctttcaatgg
     3481 tagatgtgca taggtatttg taaaccactt atataactct cctaactgat tccatttaaa
     3541 ttgatcagaa ggcgttttaa cagataatcc atttctttca tcattttccc attttagaat
     3601 taaattcgtc caaccaactt gatatgctaa attttcagca ggtgttctat ccacttcctc
     3661 aactctttta tccttcaatt cttcaggaat agtttcaaat tctaaaatat atttagtata
     3721 agtcctattt atttcttttt ttaattcttc tttattttta tatgttctca ttatattacc
     3781 tccataaatt cctaattgtc tcctttaatt catctataat ttcatccacg atattttaat
     3841 ccgttctaca atgcatatct gatatgcatt gactgtctaa ctcatatgtg aatgatttat
     3901 aaaatatccc aacgtctcat ctaaagaaat gttgatcgtc agcaaggagc taaaaaatac
     3961 gaatatattt cttttgtagt aaaatcggac tggtttgcat atcattttct tcgtaaatat
     4021 caaaatcatg ttaactacta tcaattacta ataaaaacta ccactttaag cgttaagaac
     4081 tgttaagttt agttaagtga ggatagttca caaaatccat tgttaaactt cacgaaagtg
     4141 gtaaaactca aaatgcttta tataggatta cagcatcgct ctttcttcga ttgcgcgttg
     4201 ggtaaaaaat attatgaagt aaaaattgac gatgacacgt tccttacggc tcatcagatg
     4261 aaacagctac aaaaacgaaa tgatcaactc gaagaaaatt taattctaaa aaagcgattg
     4321 ccatattcac gcctcgctcg acgaactatt aaaggtcaag atttacctga agatcatcct
     4381 ttttaggaaa ttgaagcttt ttatgttgca caatgcattt ataatactac aatgatgttt
     4441 tcaccagata ttcttctagg gtgcggtgta atgaaacaga tacacttgca aaagaaaatt
     4501 caccgcgaat ttactaaaat tatgaataat tatgttaatc ttccgaaatt tttttccaaa
     4561 attaagggat caagctggtg ttatagattg tttaaggttg gcaaaaaaat aacttctttt
     4621 taaacaatta tagaaaaccg atactctgtt aagttggttg acacggtgtc gatttttatg
     4681 actttcttat tctgaaactt cactaacgga cagtacctaa aaagtctata gtatggacgt
     4741 ttacgagtac tattttatca aaaatgctgc tcatttcaac ttatatgttt tagtgaacta
     4801 cccccactta
//
LOCUS       DASHHB010000110         4783 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.117, whole genome shotgun sequence.
ACCESSION   DASHHB010000110 DASHHB010000000
VERSION     DASHHB010000110.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4783)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4783)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4783
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.117"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(522..878)
                     /locus_tag="U2K71_002730"
     CDS             complement(522..878)
                     /locus_tag="U2K71_002730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase"
                     /protein_id="HEM7316737.1"
                     /translation="MEVKVYGSAHCVTSLKAKQWLEHHQLPYQFVDLEERDLSTEEAE
                     ELCSFEEVQAEQLFATWSEAFHNIAIDLSCVGKKQLIFLCTQHTNLLRRPLIIIDEVL
                     FIGYNEQLMEQLILNK"
     gene            complement(892..1302)
                     /gene="spx"
                     /locus_tag="U2K71_002731"
     CDS             complement(892..1302)
                     /gene="spx"
                     /locus_tag="U2K71_002731"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator Spx"
                     /protein_id="HEM7316738.1"
                     /translation="MIKVYTSPSCTSCRKARAWLQANQLEFEEKNIFAEPLTESEIKK
                     ILAATEGGVQDIISTRSKIYEKLGIDFNELTLKQMIVLFKEYPSLLRRPILIDEKRIL
                     IGFNEDEIRSFVPREIRCVARNLIYLQNNNILNS"
     gene            complement(1649..3835)
                     /locus_tag="U2K71_002732"
     CDS             complement(1649..3835)
                     /locus_tag="U2K71_002732"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HEM7316739.1"
                     /translation="MNEKEILRLQKELKRWENKYKKLQQVFRLQEEIIATYEAAYGEA
                     PGLIEEEVQVKESEGNSSFFKMMEKRSYNRFNQEQKAAITYDMKKHLRIIAGAGSGKT
                     QTICAKAVYLMTQKQVDEERILMITFTRNAANELKKRVDNFSQRKTAVHIGTFHSIFF
                     RIYNEICRKFPEVAMQGIQGDFSKDTAQKVNAVLQQLIRKYNLYIFDQYGEKTIASRL
                     DYWQNMNLSVEEMVQLVKEKYDSIDKNARQPISERLYGLLTELQEQKRSQQLLEFNDV
                     LQNLKSALENEEIQHYIGQKYDYLFIDEFQDTNPLQWEIVKRMTKENRIKLIVVGDDD
                     QSIYGFRGSEPAYIKNFEKEYPTKTLFLLTNYRSRAAIVQGANRLISYNKNDRIPKAM
                     IPAQKEAGVMEAYLFSDTKAESQWLIKQIQTFIQKNGKYKESIILYRSASQTQQLVQS
                     LLKTNIPFVLEADSPYEGIFGIKSFQHFYQKIVHWQTALNLQKKNQAYQQLLRQLMAD
                     CYLKKSECDQYFSPKYQNIAVADYILSISPNLKSKSAEIREFEEALRKTDQHQEIYSL
                     VQSYLHLPRIAKEIDQSDQEWIREEVKQHATFRRLQALSQETQATAKELKTRLIAYRQ
                     GKLDALCIQSIHKSKGLSYRNVFLIGCNEGSLPYNGATEKKVIDFNEIKAEPVTTIEE
                     ERRLFYVAMTRARNRLYLCVPQMKNSRKLKVSRFIKETGSRVKKGK"
     gene            4053..4736
                     /locus_tag="U2K71_002733"
     CDS             4053..4736
                     /locus_tag="U2K71_002733"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1275 domain-containing protein"
                     /protein_id="HEM7316740.1"
                     /translation="MQTSFHEGRTIGLLLTFIGGAMDSYTYIQYGAFASAQTGNIILS
                     IIQAFDGEWLSVGKKVLSTLFFFLGILLTKYLIDYFRKKEKHFWRLFVLYYEAVIFFF
                     VSLTPVHEHPALVTILIAFTASIQWVAFDKINGRAYTNLFTTGNLKGVATNLYDYCIS
                     KEKADFDRFFHYLSVVLAFIAGAIVLVFCHHLFGAHSILIVSILFLIMALTQTFHLWQ
                     FYHTNRFTI"
BASE COUNT         1323 a          898 c          767 g         1795 t
ORIGIN      
        1 gagtgtttcc cgattcgttt ttggctgccg tagcaacttt tatatcatat cagagagaca
       61 agcgattgtc aactcttttt taaaagtttt tttcgatttc gtttttcgaa atctttcagc
      121 aactcattta tcataactta tcgtttgtta tttgtcaaga acttttttga agtttttttc
      181 gatagaatca agcttcgcaa gtggttcttt tttaacaact cagttatctt atcatgtcat
      241 ttctaacatg tcaacaacta ttttatatgt tttttaaaat actattttat tgctttgcta
      301 cttaaaacaa cagaaatgaa tatatcatgt aaatttacac atgtcaatat atttttaact
      361 ctatatcccc acctagattt gaattttgac cacataaatt gagcatgtct ctatagtaga
      421 aaaataaagt caaaaaaaga acacataaca cgcttgatgc taagtgttcg actgttacaa
      481 ctgtatgaaa tgctcagtga cttgattaaa acagttaaag tctatttatt taagataagc
      541 tgttccatca actgctcatt gtatccaata aatagaactt catcgataat aatcaaagga
      601 cgtctgagga gattggtatg ttgtgtgcat aaaaaaatca gttgtttttt tcctacacag
      661 gaaaggtcaa tcgcaatgtt gtgaaaagcc tcagaccaag tcgcaaatag ctgttctgct
      721 tgcacttctt caaaactgca taattcttct gcttcttctg tacttagatc gcgttcttct
      781 aagtcaacaa attgataagg caattgatgg tgttctaacc attgtttcgc cttcagactt
      841 gttacacagt gcgctgatcc atagactttc acttccacga aggctccctc cctatgaatt
      901 taagatgtta ttgttttgta aatagatcag atttctggca acacatcgaa tttcacgagg
      961 gacaaatgac cggatctcat cttcattaaa tccgattagt attctttttt catcaatcaa
     1021 aataggtcgt ctcaataatg aaggatattc tttgaataag acgatcattt gctttaaagt
     1081 taattcgtta aaatctatgc ccagcttttc gtaaattttc gaccgagtgg aaataatatc
     1141 ttgaactcct ccttcagtcg ctgccaagat ctttttgatc tcactttctg ttaacggttc
     1201 ggcaaaaata ttcttctctt caaattcaag ttgatttgct tgcaaccatg ctcttgcttt
     1261 tctacaggaa gtacagctag gcgaagtata tacttttatc attttctcac tcatttttaa
     1321 ttataatcca attctacagt aatttctttt aaaataccaa ttattagcta ataatatata
     1381 aaaaaaaatc aaaataactg attatttacg tttgaataat gagaattttt ctatatattc
     1441 tctattttga taatagtatt ctcattttca aaataaataa gggaggtgtg acaaaattct
     1501 tgtcacacct cccttttccg aataaactgt gttccagaaa tagcttcttc gaaaataagc
     1561 tgaaatttca caaaaatttg agaagcaatt ttcgtacatt ccttcttctt tcttgaaact
     1621 gaccacttct gtcacaccct ctcttctatc actttccttt tttcacacga ctcccggttt
     1681 ctttgataaa tctcgatact ttcaattttc ttgaattttt catttgaggt acacatagat
     1741 acagacgatt tcttgctcta gtcatcgcaa cataaaatag tcttcgttct tcttctatcg
     1801 tcgtcactgg ttctgctttt atttcattaa agtcgattac tttcttttct gtcgctccgt
     1861 tataaggcaa acttccttcg ttacaaccaa tcaaaaagac atttcggtaa ctaagacctt
     1921 tacttttgtg aatgctctgg atacatagtg catcaagttt tccttgtctg taagcaatca
     1981 agcgggtttt taattcttta gctgtcgctt gggtttcttg cgataaggct tggagtcttc
     2041 tgaaagtagc atgctgcttt acttcttctc gtatccattc ttgatcactc tggtcgattt
     2101 ccttagctat acgtggcaga tgaagatagc tttgaacaag cgaatagatt tcttgatgct
     2161 ggtctgtttt acgcaacgct tcctcaaact ctcggatttc tgcagatttc gattttaaat
     2221 tggggcttat cgacaaaata taatctgcaa cggctatatt ttgatattta ggagaaaagt
     2281 attgatcgca ttcacttttt ttcaggtagc agtctgccat cagctgccgg agcaattgct
     2341 ggtaggcctg atttttcttt tgcagattca gtgctgtttg ccaatgaaca attttttgat
     2401 agaaatgttg gaaagacttg ataccaaaga taccttcata tggactatct gcttccagta
     2461 caaaaggaat attcgttttc agcagagatt ggacaagctg ctgcgtttgt gaagctgatc
     2521 ggtataatat gattgattcc ttatatttcc catttttttg aataaatgtc tgaatttgtt
     2581 tgattagcca ctgactttct gctttagtat cagaaaacaa ataggcttcc ataacacctg
     2641 cttctttttg tgctggaatc atcgcctttg gtatccgatc atttttatta tagctgatca
     2701 atctatttgc tccttggaca atagctgccc gagaacgata attagtcaat aagaaaagag
     2761 ttttggtagg atattctttt tcaaagttct tgatgtatgc tggctctgat cctctgaatc
     2821 catagatact ctgatcatca tctccaacaa caatcaactt gatcctgttt tcctttgtca
     2881 tccgtttcac gatttcccat tgaagtggat tagtatcttg aaattcatca atgaatagat
     2941 aatcatattt ttggccgata taatgctgga tctcctcatt ctccaaagcg cttttcagat
     3001 tttggagtac atcattgaac tctagcaatt gctggcttct tttttgttct tggagttccg
     3061 tgagcaatcc atacagtcgt tctgatatcg gctgtcgtgc attcttatca atggaatcat
     3121 atttttcttt aactaactga accatttctt ctactgaaag gttcatattc tgccaatagt
     3181 ctaaccgaga agcaatcgtt ttttctccat attgatcaaa tatataaaga ttatatttac
     3241 gtatcagctg ttgcaaaaca gcattcactt tttgtgcagt atcctttgaa aagtctcctt
     3301 gtattccttg cattgctact tcaggaaatt ttcgacaaat ttcattgtat atcctaaaaa
     3361 agatactatg gaatgttccg atatggacag cggtttttct ctggctgaaa ttatctactc
     3421 gtttttttag ttcatttgca gcgttccttg taaaagtgat cattagtatg cgttcttcgt
     3481 ctacttgttt ttgcgtcatc agataaacag cttttgcaca gattgtttgc gttttgccgc
     3541 tcccagctcc tgctatgatt cgaagatgtt tcttcatatc atatgtgata gctgcttttt
     3601 gttcttgatt gaagcgatta taggaacgtt tctccatcat tttaaagaaa gagctgtttc
     3661 cttcactttc ttttacttgt acttcttcct caataagtcc gggtgcttcg ccataagccg
     3721 cttcataggt agcaatgatt tcttcttgta gccgaaagac ttgttgaagc tttttatact
     3781 tgttctccca gcgtttcaat tctttttgta atcggagaat ttctttttca ttcaactgat
     3841 tgatttcctt cccatctttc ttctttgaat ttgtttattt caagttggtt atttaaagtt
     3901 tcgttgtcta ttttttcttt atactaacag acctttctaa aaatgaaaat gggaagcgat
     3961 atgattttag ttttctgaaa atttttcctt tttttcaatc gtttatgcta agataaggaa
     4021 gacaaaaata ttcataaaag gtgatgaaaa aaatgcagac ttcctttcac gaggggcgta
     4081 caataggact tttgctgaca tttatcggag gagccatgga cagttatact tatatccaat
     4141 atggtgcttt tgcctctgct caaactggca atatcattct atcgatcatc caagcatttg
     4201 acggagaatg gctaagtgtt ggtaaaaaag tattgtctac ccttttcttt ttcttaggga
     4261 ttcttctaac aaagtatcta attgattatt ttagaaaaaa agaaaaacat ttctggcgct
     4321 tgtttgttct atattatgaa gcagtgattt ttttctttgt tagtttgaca cccgtccatg
     4381 agcatcccgc attagtaacg attttgattg cttttactgc atcgatccag tgggtagctt
     4441 ttgataaaat caacgggcgt gcctacacta atttatttac aactggaaat ttaaaagggg
     4501 tagcaacgaa cttatatgat tattgtattt ctaaagaaaa agcagatttt gatcgatttt
     4561 tccactatct aagtgtcgtt cttgccttta tcgcaggagc tattgtcctt gttttctgtc
     4621 atcatctgtt tggcgcccat tcgattttga tcgtttccat tttatttttg atcatggccc
     4681 tcactcagac atttcattta tggcaatttt atcatacgaa tcggttcact atttgatctc
     4741 aatgaatcaa aaaaactaag caagatgctg ctcacagaga aat
//
LOCUS       DASHHB010000111         4623 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.118, whole genome shotgun sequence.
ACCESSION   DASHHB010000111 DASHHB010000000
VERSION     DASHHB010000111.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.118"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(584..1402)
                     /locus_tag="U2K71_002734"
     CDS             complement(584..1402)
                     /locus_tag="U2K71_002734"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002313161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HEM7316741.1"
                     /translation="MSYIYLIKENYPFLTKSEKKVADFILNSGKTIIYSTMSDIKKNT
                     KVGDATIIRFCQKIGFSGFSDLKIEIAKEDFSQKKEHPTSVKYYDEIANSLTEALYST
                     IRLLDEEKLNEAIKMITQSKNIYIFGVGSSGNTSLDLENMFLRVGVQAKAVLDPHFQA
                     QVASLLTVNDLVIIFSLSGKTKDTYDSLKIAKKNGAKIIAITNYIHSPIGKSADLVLQ
                     TAIEEFLNGGSLAGKISQLYICDLLVHGYEQNNNIDSVELREKVLRSIIDKSIE"
     gene            1563..2252
                     /locus_tag="U2K71_002735"
     CDS             1563..2252
                     /locus_tag="U2K71_002735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002358533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmannosamine-6-phosphate 2-epimerase"
                     /protein_id="HEM7316742.1"
                     /translation="MDKKRFLDLISKGLIVSCQALPGEPFYKEEESLMPYFALAAQRA
                     GAVGIRANSVRDIIEIRKCVSLPMIGIIKRDYPPESPFITATMREVDELVETGVEVIA
                     LDCTLRNRHDGLSINSFITKIKEKYPHQLFMADISTFEEGKNAYKAGIDFVGTTLSGY
                     TEESKKQDGPDIDLIKKLVEEGICVIAEGRIHTPEQAKEIKKIGVAGIVVGGAITRPQ
                     EIASRFVKALS"
     gene            2266..3768
                     /locus_tag="U2K71_002736"
     CDS             2266..3768
                     /locus_tag="U2K71_002736"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002367389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM7316743.1"
                     /translation="MFKKFSQLGRAFMLPIAILPVAGLLLGLGGALTNESAINAYPFL
                     DQPWLHTILSIMSYAGNAVFANLALIFAIGIAVGLANGDKGTAGLAGGVAYLVYTATI
                     SGFLELFSAKDATLDTGVVGSIVVGGTVAFLHNRYRKIELPQFLGFFGGSRFIPIVSS
                     LAAIIIGSFFYLIWPPIQNGLVVAGEQIAQMGSLGTFLYGFLLRLTGAVGLHHTIYPL
                     FWYTSLGGTETVAGSTIAGAQNIFFAQLADPNHTGLFTYGTRFFAGRFATMMFGLPAA
                     CYAMYRAIPKKNRKKNGGLYFSGALTSFLTGITEPVEYMFLFVAPWLYVIHAFLDGLS
                     FYFADILNIRVGNSFSGGLIDFLLFGVLQGNDKTNWLKLIPFGIAWAFIYFFVFTFFI
                     KKFKVAIPGMEEESEVHSEKVINKNTSLHEESLIIIEALGGKENIESVEACATRLRVA
                     VTNGEIIQKQTIKDTGATAVFEVKGGIQAVFGGKADLLSQEINQILGKDN"
     gene            3781..4257
                     /locus_tag="U2K71_002737"
     CDS             3781..4257
                     /locus_tag="U2K71_002737"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM7316744.1"
                     /translation="MFEIFKKKKQETFYAPCSGKIISITEVNDPVFSQKLLGDGFAVI
                     PDGSKIYAPLAGRVTNIFPTKHAINIKSNSGIDYLIHIGIDTVELQGEPFSICVQEND
                     EVTPETSLVDVDFDKIIKSGKDTSVIVVFPEKNQFNDLIIDNGRIAHGNSCGKITF"
BASE COUNT         1546 a          665 c          817 g         1595 t
ORIGIN      
        1 aatataaaaa tcaaaaggat cataaacttt taacaagtga tgaaatgggt ggaaattgat
       61 tcttatagac agtttgtctc ataacattga agtattttgc aattttagca ttgaaacact
      121 tttttaatct tcaacgttgt tcttataaac aagatgattg ggaatctttt gaattaacac
      181 tgtatagctt agaacaccct ttatagaaaa cattcgctta gcaatcttaa taattccttg
      241 tgcccgatgt cgtttagatt cagtaggttc ctgctcatcg atcatggaaa aaatttgaag
      301 gatcagatcc ttaataaatt tatttaagag aggatgatcc attgcttcat ccatactcga
      361 taaactagtg ataatgagct acacgttttt ctttcaagtt gatcaatagc ttccacgata
      421 aaaatatctt tagataaaat caagaaccgt aggaactaca gggatatttg gtcaataaga
      481 gccaccgcta ttagtaaaaa ataagctatc aagtatgctc tcatcccaag aattgcctac
      541 ttcaatcgtt aaaaaccgtt aggtttagct aagtgggggt agttcactca atacttttat
      601 caataataga gcgcaaaact ttttctctta attcaactga atcaatatta ttgttttgtt
      661 cataaccatg aactaataga tcacaaatat aaagttgtga tatttttcca gctaaagatc
      721 caccattaag aaattcttca atggctgttt gtaaaactaa gtctgcagac tttcctatag
      781 gtgaatgaat atagttagtt atagcaataa tttttgcgcc atttttttta gctattttca
      841 atgaatcata agtatctttc gttttcccag ataaagaaaa aatgattact aaatcattaa
      901 ctgttaataa agaagcgact tgggcttgaa aatgagggtc tagtacagct tttgcttgaa
      961 ctcctacgcg aagaaacata ttttctaaat caagactagt atttccacta gatcccactc
     1021 caaatatata tatgtttttt gattgagtga tcattttaat ggcttcattc aatttttctt
     1081 cgtctaaaag cctaatagtg gaatagagag cttctgttaa actattagca atttcatcat
     1141 agtactttac actagtcgga tgttcttttt tttgactaaa gtcttccttt gcaatttcaa
     1201 tttttaaatc agaaaaacca gaaaaaccaa ttttttgaca aaagcgaata atagtagcat
     1261 cgccaacttt tgtgtttttc ttaatatcac tcatcgtact ataaataata gttttcccag
     1321 aatttaaaat aaaatcagcg actttttttt cagacttcgt taaaaatgga taattttctt
     1381 taattaaata gatataactc atacaaactc ctctacaatt tcaacttttc tccattatac
     1441 tccaaaataa taaaaaaaac tcctaaatac tccattttta tctttacttt tatgaaagcg
     1501 gatgctatat tgtttttgga gttgttatcc aaaaataata aaaaattgga gtgtttagga
     1561 taatggataa aaagaggttt ttagatctta tttcaaaagg tttgattgtt tcctgtcaag
     1621 cattacctgg tgaaccattt tacaaagagg aagaaagttt aatgccttat tttgctttgg
     1681 ctgctcaaag ggctggagca gttggtatac gtgcaaattc tgttcgagat attattgaaa
     1741 ttagaaagtg tgtatcatta ccaatgattg gaattattaa aagagattat ccaccagaat
     1801 ctccattcat tactgccact atgagagaag tagatgaatt agtagaaacc ggtgttgagg
     1861 taatcgctct tgattgcact cttagaaatc gacatgatgg actatctata aattctttta
     1921 ttactaaaat aaaagaaaaa tatcctcatc agttatttat ggcagatatt tctacatttg
     1981 aagaaggcaa aaatgcatat aaagcaggaa tagattttgt aggaactact ttaagtggat
     2041 atactgagga aagtaaaaaa caagatgggc cagatataga tttgattaaa aaattggtag
     2101 aagaagggat ttgtgtaatt gctgagggaa ggattcatac tccagaacaa gcaaaagaga
     2161 taaaaaaaat aggagttgca ggtattgttg tgggtggtgc tattactcga ccacaggaaa
     2221 ttgcatcaag atttgttaaa gcattaagtt agggggagaa ataaaatgtt taaaaaattt
     2281 tcacaattag gacgcgcttt catgttacca attgctatat taccagtagc cggattacta
     2341 ttgggactgg gaggagcgtt aacaaatgag agtgctataa atgcttatcc atttttagat
     2401 caaccgtggt tgcatacaat tttaagtatt atgagttatg ctggaaatgc agtttttgca
     2461 aacctagcac taatttttgc aattggtatt gctgtaggac ttgcaaatgg agataaagga
     2521 acagctggtt tagctggtgg agtagcttat ctagtatata ctgcaactat tagtgggttt
     2581 cttgaacttt tttcagcaaa agatgcaaca ctagatacag gtgtagtggg atcaattgtt
     2641 gttggtggta ctgttgcatt tttacacaat cgttatagaa aaattgaatt accacaattc
     2701 ttaggttttt ttggaggatc aagatttatt ccgattgttt ctagtttagc tgcaatcatt
     2761 attggttctt tcttttattt aatttggcca ccaattcaaa atggtttagt agttgctggt
     2821 gaacaaattg cccaaatggg tagtttagga acttttttat atggtttttt attgcgttta
     2881 acaggtgctg ttggactaca ccacaccatt taccctttgt tctggtatac atctttaggt
     2941 ggtaccgaaa ctgtagctgg atcaacaatt gctggagccc aaaatatttt ctttgcccaa
     3001 ctagctgatc caaaccacac tggtctgttt acttatggta ctagattttt tgcaggaaga
     3061 tttgcaacaa tgatgtttgg attaccagca gcatgttatg caatgtatcg tgcaattcct
     3121 aagaaaaatc gtaaaaagaa tggtgggctt tattttagcg gggcccttac atcattttta
     3181 actggaatta ctgaaccagt tgaatatatg tttttatttg ttgctccttg gctttatgtg
     3241 attcatgcat tcttagatgg attatctttt tattttgctg atatcttaaa tattcgtgta
     3301 gggaattcct tttcaggtgg tttgatcgac tttttattat tcggtgtact tcaagggaat
     3361 gacaaaacta attggttaaa attaattcct tttggtattg catgggcatt tatatatttc
     3421 tttgttttta cattctttat caaaaaattt aaggtagcta ttcctggaat ggaagaagaa
     3481 tcagaagttc attctgaaaa agtaataaac aaaaatacat cattacatga agagtctctt
     3541 atcattattg aagctcttgg tggaaaagaa aatattgagt ctgttgaagc ttgtgcaact
     3601 cgtttgcgtg ttgctgttac gaatggggag attattcaaa aacaaacaat aaaagacact
     3661 ggtgcaactg ctgtcttcga agtgaagggt ggcatccaag ctgtttttgg agggaaagct
     3721 gacttattaa gtcaagaaat caatcaaata ttaggaaaag ataattagga ggtttttaat
     3781 atgtttgaaa ttttcaaaaa gaaaaaacaa gaaacttttt atgcaccttg ttccggaaaa
     3841 atcatttcca tcacagaagt aaatgatcct gtcttttcac aaaaattact aggtgatggt
     3901 tttgccgtca tacctgatgg ttcaaaaatt tatgctccat tggctggtcg agtaacaaat
     3961 atatttccta ctaaacatgc tattaatata aaaagcaact caggcattga ttatttgatt
     4021 catattggaa ttgatacagt tgaattacaa ggagagcctt tttcgatttg tgttcaagaa
     4081 aatgatgagg ttactcctga aacctcgttg gttgatgtgg attttgacaa aataataaaa
     4141 tctggtaaag atacttctgt tatagttgtt tttccggaga aaaatcagtt caatgattta
     4201 ataatagata atggtcgaat tgctcatggg aattcatgtg gaaaaatcac tttttaaaaa
     4261 agtgtatata aaaagctata aaataagcta aataggatat ttgtcaaatg aagttggtga
     4321 acagaaagaa ctgttttctt tctgttcatt tttttatttt attagtaatg attccccatg
     4381 ccaaatcatg atccgtaatt taaagttata aaagcttcga aatccattgg catttccttg
     4441 gaataccttg atatgattat tcaaatataa aatatagaga gaagagagag agggaagata
     4501 tttataaagg gcaaagttat gtttactcaa cttaggaaat agaaatagat caatttatat
     4561 tctcttgtca atcgaatatg aaccttagca ctatatacca aatatagtaa aatgctgaaa
     4621 gat
//
LOCUS       DASHHB010000112         4587 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.119, whole genome shotgun sequence.
ACCESSION   DASHHB010000112 DASHHB010000000
VERSION     DASHHB010000112.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4587)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4587)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4587
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.119"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            186..2855
                     /locus_tag="U2K71_002738"
     CDS             186..2855
                     /locus_tag="U2K71_002738"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfhO family protein"
                     /protein_id="HEM7316745.1"
                     /translation="MKKNLKSFFRENGRYAAASFLIPFLILALVYLSIGIYPGSSRSI
                     LASDAFSQFSNFHASFRNVLLGKQSIFYTWNASLGLNYLSLISYYLGGIFTPLVFFFP
                     NQMMPDALYVLTLLKVGCAGLAFWFLARTFKIPRWGHVALSVSYALISFITAHSEIIM
                     WLDAFVYLPLVILGIHRVMDLKRPKLLFISYLLLFLSSFYMGFMIGVFSFLYFIARLL
                     TNWSEYKKTIVPYGITSLLAGGASMIIVLPAVLDLRSNGEALSQITTLKTEATSYLDL
                     IMKNMIGVYDTTKYGSIPFVYAGLLPLVLCLFYFASRKVPLKNKFLYGSLFAILIASF
                     YLVPLNLFWHGMHAPNMFLFRYAYLFSFLVLLLAGKGWEKIEQDDRGLLLGIILVVAA
                     LFAVAWGIKPEGSYTYVTMTSFVLTVVFLGIYAFAVGFYQHKKLSFNHLSILLLLIMS
                     AEALVNTNSMIHGILDDWNYASRSLYTDPYPDLKALVNETKKDSDSFYRLENLDPVSA
                     NDSINYGYSGISLFSSIRNRNSSSYLDKLGFRSRGTNLNIRYNNNTLLMDGFTGIKYN
                     ISKNESTFSKYGFRQAGQSGDYTLYQNVNALPLAFTAPLSVNDVEQPVTDNLTSQTNL
                     INTLSGLNEQYFTFYTPTLKNQLNTTITKTTTGVTYSETADNQPKELTWEVHVPANTQ
                     AYLSLFPTNFAQLESSSATITVNGTSRKTQINISGQYYDLGYYPQETTVTFTASFYGT
                     KEVSFMEPKVVGLDVVAYQAAMNQIKEKGVEMEATGRKASGTVNTAEDSMLVTTIPYD
                     KGWKAKIDGKPVKVSNFKDAFLMIKVPAGKHTVTFSYLPEGFTIGAILFVLCIALFIL
                     YVWYLDRNRPFYGTIDPAIEEKPRNTHRRRRRK"
     gene            complement(2946..3566)
                     /locus_tag="U2K71_002739"
     CDS             complement(2946..3566)
                     /locus_tag="U2K71_002739"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HEM7316746.1"
                     /translation="MFLINATDEILSVNKVFYTVPAEKMSRVSSDINTITSRIGLTKA
                     MHMVLKYNQEDPPVPKTYEAIDLIIVDEIDRLKLQHLEQLRDIYDRNDLAMVLIGMPG
                     IEKRLSRYPQLYSRIGFAHEFDNLSKDETHHILEYKWADLGIDIKLEDFSDYEAITTI
                     IKITKGNFRLIHRLFAQIDRILRINQMDKITVEVVEAARDSLVIGI"
     gene            3680..4219
                     /locus_tag="U2K71_002740"
     CDS             3680..4219
                     /locus_tag="U2K71_002740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316747.1"
                     /translation="MKKLFSALFILLFGFIGIGTSGNIVKASTMVEQTSEMAKTFSQL
                     TPEEQSYFKSKGFSDKDTFYTQKIEPQDPKTRFSANVLRLTASTKKITNTQGRTEYII
                     TSPNSAMYRISTRLSLGSAKSYTQTVNLANSYGYGGGMYFTFTGKRQYYSVKLAMQVY
                     NTFGVGSVSCTAGGLTIGK"
     gene            4216..4554
                     /locus_tag="U2K71_002741"
     CDS             4216..4554
                     /locus_tag="U2K71_002741"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic metallochaperone ArsD family protein"
                     /protein_id="HEM7316748.1"
                     /translation="MIISYYVLNRSDDEGLFVFQSLHEDTMLKEFYNRIKNKQIETNL
                     YFLSDSILPFISNPKILNLLQTKDLKSLPIIVIDEEIVKYGAYLSVEELEKLTNIGMS
                     IQEDNGVHGT"
BASE COUNT         1419 a          790 c          860 g         1518 t
ORIGIN      
        1 atttttaagg gtgggacttt tgattcctac cctttttttg cgtttatttt tgttatacga
       61 acaaattatg aagttattta ttctaaattt tatttttttg tgaaaattag actcttttta
      121 agcagagaaa aacaaggata tgataaactg aatacaactt catagaaatt tggtggaaaa
      181 taaatatgaa aaaaaattta aagtcattct ttagagaaaa cggacgctac gcagcagcta
      241 gtttcctgat tccgtttttg attttagcac ttgtttatct aagtatcggg atctatcctg
      301 gaagcagcag aagtattttg gcaagtgatg ccttttcaca gttttctaat tttcatgcca
      361 gcttccgaaa tgttcttctt ggaaagcaaa gtatttttta tacatggaat gcttctctag
      421 gattgaatta tttatcttta atttcttact atctaggcgg gatcttcacg ccactggtgt
      481 tcttttttcc aaaccagatg atgccagatg cactgtatgt tctgactttg cttaaggtcg
      541 gctgtgccgg attggctttt tggtttcttg cacgtacgtt caagattcct cgctgggggc
      601 atgttgcact tagcgtcagt tacgcattga tctcatttat cactgcacat tcggagatca
      661 tcatgtggct ggatgcattt gtttatcttc cattagtgat tttggggatt catcgtgtta
      721 tggatcttaa acggccgaaa cttttattta tcagttattt actcttgttt ttatccagtt
      781 tttatatggg ttttatgatt ggcgtatttt ctttcttata ttttatcgcc cgtctattga
      841 caaactggtc agaatataaa aaaacgattg ttccttatgg tatcacttct ttactggcag
      901 gcggcgcttc aatgatcatt gtattgccag cagtgctgga tcttcggtca aatggcgagg
      961 cactgagtca gatcactacg ttgaaaacag aagcgacttc ttatctggat ctaatcatga
     1021 aaaatatgat tggtgtttat gacacgacaa aatacggatc gattccgttc gtttacgcag
     1081 gtctgctacc attagtcctc tgtctgtttt attttgccag tcggaaagta ccgttgaaaa
     1141 ataaattttt gtacggcagt cttttcgcca tacttatcgc aagtttctat cttgttccat
     1201 tgaatttgtt ctggcatggt atgcatgcac cgaatatgtt tttattccgt tatgcctatc
     1261 tgttctcttt cttagtgctc ctgcttgcag gaaaaggatg ggaaaagata gaacaagacg
     1321 atcgtgggct gttgttagga atcattttgg tcgtagctgc tctctttgcg gtggcatggg
     1381 gcatcaaacc tgaaggaagc tacacctatg tgacgatgac ttcctttgta ttgacagtgg
     1441 ttttcttagg gatttatgct tttgctgtgg gattttatca gcataaaaaa ctttcattca
     1501 atcatctgag tatattgtta ttattgatca tgtctgctga agcactggtc aatacgaaca
     1561 gtatgatcca tggcattctt gatgattgga attatgcttc ccgcagttta tatactgatc
     1621 cttatcctga cttgaaagct cttgtaaatg aaacaaaaaa agattccgac tcgttttacc
     1681 gcttggaaaa tctggatcca gtatctgcaa acgattcgat caactatggt tacagtggta
     1741 tcagtttgtt ttcttctata agaaatcgta attcttcctc ttacctggat aaattaggtt
     1801 tccgttcaag agggacaaat ttgaatatcc gttataacaa taatactttg ctaatggatg
     1861 ggtttacagg aatcaaatat aacatttcta aaaacgaaag cacgttcagt aaatatggct
     1921 tccgccaagc tggtcaaagt ggcgactata cactgtatca gaatgtcaat gcattgccac
     1981 tagcatttac tgcaccgttg tccgttaatg atgttgagca gccagtgaca gataatttga
     2041 ccagtcagac aaacttgatc aatacattgt ctggtttaaa tgaacagtat tttacattct
     2101 atacacctac tttgaagaat cagttgaata cgacgatcac aaagacaacg actggcgtca
     2161 cttactcaga aacagcagat aaccagccaa aagagctcac ttgggaagtc catgtgcctg
     2221 caaatacaca agcttactta agtttgttcc caaccaattt tgcacaatta gaaagttcta
     2281 gtgcgacgat cactgtcaat ggtacatcgc gaaaaacgca aatcaacatt tctggtcaat
     2341 actatgatct tggctattat cctcaagaga caacagtgac atttactgca agtttttatg
     2401 gaacgaaaga agtaagtttc atggagccta aagtcgttgg tctagatgtt gtagcctatc
     2461 aagccgcaat gaatcagatc aaagaaaaag gtgtcgaaat ggaagccact ggcagaaaag
     2521 cttctgggac tgtaaatacc gctgaagatt ccatgttggt gacaactatt ccttatgaca
     2581 aaggatggaa agctaaaatt gatgggaaac cagtcaaggt cagcaatttc aaagacgcat
     2641 tcttgatgat aaaagttcca gcaggtaaac atactgtcac tttcagttac ttgcctgaag
     2701 gatttacgat aggtgcaatc ctttttgtcc tatgtattgc attgtttatt ctttatgtct
     2761 ggtaccttga ccgcaatcgc cccttttatg gcacgattga tccagcaata gaagaaaaac
     2821 cgcgaaatac acacagaaga agacgaagaa aataaagaaa cgatggaatc tgtgtcactc
     2881 ttctatttaa atggcaaaaa tttcaatctt taaatgacaa aaaccacctg atttttggtg
     2941 gttttttata tacctatgac taaactatct cttgctgctt cgacaacttc tacagtgatc
     3001 ttatccattt gattgattct taaaatccga tcaatctgcg caaataaacg atgaataagc
     3061 ctaaaatttc cttttgtgat cttaatgata gttgtgatag cctcataatc agaaaaatct
     3121 tctagcttga tatcaattcc taaatctgcc catttgtatt ccaaaatatg atgtgtttcg
     3181 tccttactaa ggttatcaaa ttcatgtgca aatcctatgc gagagtaaag ctgcggataa
     3241 cgagacaatc ttttttcaat accaggcata ccgataagaa ccattgccag atcatttcta
     3301 tcgtaaatat ctcttagctg ttctaaatgc tgaagcttta aacgatcaat ttcatcgaca
     3361 ataattaagt caattgcttc ataggtttta ggcacaggag gatcttcttg attgtatttt
     3421 agtaccatat gcattgcttt agtcagacct attctactag ttattgtgtt aatatccgaa
     3481 ctgacacgag acatcttttc agctggtaca gtataaaaaa ctttatttac acttaatatt
     3541 tcatctgttg catttatcaa gaacaagaaa aagtctgaat attcaaaaaa aataacggtg
     3601 tacattttta aaaggagatg tataatcgag atttagaaag cgttttcctc aaaatttaat
     3661 gaattaggaa gtgattacta tgaaaaaatt atttagcgca ctttttattc tgttatttgg
     3721 ctttattggt attgggactt ctggaaatat agttaaagct agcacgatgg ttgaacaaac
     3781 aagtgaaatg gcaaaaactt ttagtcaatt aacaccagaa gaacaaagtt acttcaaatc
     3841 caaaggattt agtgataaag atacttttta cactcaaaag atagagcctc aagatccgaa
     3901 aacacgtttt tctgcaaatg tattgcggtt aaccgcatct actaaaaaaa ttactaatac
     3961 gcaggggcgg acagaatata ttataacaag tcccaattcg gcgatgtatc gtatttctac
     4021 acgcttgagt ttaggtagtg caaaatcata tactcaaaca gtaaatctag ctaattctta
     4081 tggttatggt ggaggaatgt attttacatt tactgggaaa agacaatatt acagtgtaaa
     4141 attagctatg caagtttata atacatttgg tgtagggtca gtctcttgca ctgcgggagg
     4201 attgacaata ggaaaatgat aatttcttac tatgtcttaa atagaagtga tgatgaagga
     4261 ctcttcgttt ttcaatcatt acatgaggat acgatgctaa aagagtttta taatcggatt
     4321 aagaataaac agatagaaac aaatttatat tttttaagtg actctatctt gccttttatt
     4381 agcaacccta aaattcttaa cttgttgcag acaaaagatt tgaaaagttt accgattatt
     4441 gtcattgatg aggaaattgt aaaatatggc gcttatctaa gcgtagaaga attagaaaaa
     4501 ttaactaaca ttggaatgag tattcaggag gataatgggg tacatggaac atagaatgaa
     4561 tatgcacagt tgagaacgga atttgac
//
LOCUS       DASHHB010000113         4478 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.120, whole genome shotgun sequence.
ACCESSION   DASHHB010000113 DASHHB010000000
VERSION     DASHHB010000113.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4478)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4478)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4478
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.120"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(833..1126)
                     /locus_tag="U2K71_002742"
     CDS             complement(833..1126)
                     /locus_tag="U2K71_002742"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2087 domain-containing protein"
                     /protein_id="HEM7316749.1"
                     /translation="MVYTYTEQEKMKVLDTFMNDGKVKQVPSSTKKKYILLKEVLKRF
                     DHGVTYTETEVNSILLNVFSSGDYVEQRRYLITFGFFKRSSDGRAYQMMGIEN"
     gene            complement(1529..2707)
                     /locus_tag="U2K71_002743"
     CDS             complement(1529..2707)
                     /locus_tag="U2K71_002743"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002354626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM7316750.1"
                     /translation="MEKTDRFKKQISSTGRITWYFQAYRTTRRGFNSKREAQLAYLEM
                     EKQQRLKKKIVASNDKFSVVGEQWFQYYRSLNEQKEITYDKREEQLKIIVRWIGDKKL
                     SDLTPECLQELLFTLKECGVNGTNVGYAKNSLQSLIQVLNMIFKYCQRKNLISENPMK
                     SVRMPKYQLTVTDLKEAINNIEDKFLTVDELRTFLNYGIVHEELPMSVLFHVLFYTGC
                     RVSEALALQPGDIDFKRNEILFYKQTAVKGKSKDFRIETTKTVSSARRVLVTPLVMEK
                     LQQLIKALGEMRQHYRFIIEETYLFVYLDSSKRGVPYRREYVNDHVKRCVNRSGINKP
                     FHTHLARHTMASLVAEHCSWEVLKDRLGHTDQTTSKIYRHLTSNEKVKPLIAFSSLES
                     "
     gene            complement(2811..3125)
                     /locus_tag="U2K71_002744"
     CDS             complement(2811..3125)
                     /locus_tag="U2K71_002744"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002300928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7316751.1"
                     /translation="MELEVKTNVNSLEIEFYSILQKKLEDYTEKLLAVSHLPLVLTNT
                     DLKRLFQISDSTLNRLVKLGEFPPCWYGIRGHYFRDDVLEWMRKSDSNDYREKLRLLR
                     SL"
     gene            3232..3447
                     /locus_tag="U2K71_002745"
     CDS             3232..3447
                     /locus_tag="U2K71_002745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016613890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316752.1"
                     /translation="MKQGIADIKIIKEILEKSTANAIAFGTGINLSTVKKLKSGERAE
                     EKLNLADAIKITEFGMKNMPTKIEIWK"
     gene            complement(3747..3989)
                     /locus_tag="U2K71_002746"
     CDS             complement(3747..3989)
                     /locus_tag="U2K71_002746"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7316753.1"
                     /translation="MKQNKRYIPFIPLTTIKAAASGDSIAMSIVLSKYQNYISRLAMK
                     PVYEEEKGMVMYVDEYMRRQLETKLIEKILEFDTTR"
     gene            complement(3976..4404)
                     /locus_tag="U2K71_002747"
     CDS             complement(3976..4404)
                     /locus_tag="U2K71_002747"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM7316754.1"
                     /translation="MGDSEETHIQHTFDSFCKKVVCNEALNIQKKYARFRQRQISMEI
                     LEQKGLGTEFYYLEPNLNSSGTFIVLGMKIFISNEELANAIAALPSQRQEIIFMSFFI
                     GLNDREIGDIIGKSLGSVWYQRQMALEELGKYLGVKYEAK"
BASE COUNT         1419 a          841 c          663 g         1555 t
ORIGIN      
        1 aataaaaaat catcaaataa tcctttacac gttattctaa atttaatttt tattatttta
       61 ttgatgtata gaaagtttgg ggcttttttt ctctttttat tctaaggtta ttttagtctt
      121 gtcctttaat acaaagattt tatttttaaa taaaaaatac tcttttttta cgattattga
      181 aaaaaaaaga taaaacttga ttaattgaag tattgcttaa atttggaagc tatgagggaa
      241 gaataaaaga gttaggacgg gattatctac tatttttctt tgttttaaat atttgttatc
      301 tttgaacata aaagagaaat gtgtcgactg cagtgttgtg tccctcatca cgaataaacg
      361 gtgttcaatt agctgatctt gtattcactt aaaaaatggt aaagtacgag ttgctattcc
      421 ccattttcaa ttttctaacg ctcaggacaa aatactgttt tcacaacctc attggaataa
      481 aagcatatat attgtttcaa gaacgaaatc ttgttatctt tttaaagggc atctgtccaa
      541 aatctaattt taggaggtac gttttggtat ttattcgtac aaataaaaga gaattatctt
      601 tattcatgta acgcatccaa ttgatttttt catgtacgct cctggaacga ttttactgac
      661 gattgcttat ttaactgcga atcgctcatt agcttttatc atagctatcc atattttgaa
      721 taacgttctt ggctttgtat tatagataca gatgacaaaa aaagttcaag tatgcggtgt
      781 agaaccaata tcgcatactt gaactttttt caatgagtat aaattaaaac ttctaatttt
      841 caattcccat catctgatat gcccgaccgt ctgaacttct tttgaaaaag ccaaatgtga
      901 ttagataacg tctttgttcg acataatcgc cactagaaaa tacgttcagc agtatcgaat
      961 taacctctgt ttctgtataa gtcactccat ggtcaaagcg tttcaagact tctttcaata
     1021 ggatgtattt cttttttgtt gaacttggta cttgttttac ttttccatca ttcatgaatg
     1081 tgtctaaaac tttcatcttt tcttgctccg tataagtata aaccatcttc tttctccttt
     1141 tgattctaag aatactccat ttatgttaga atattctgga ttttttatta aagcggttac
     1201 tcttaagaaa gatattatca tagtgctctt ttttttacta ctacttatag gtagatttat
     1261 tctaatccat tattctcccc taccaataat taaagaaaaa actggatcag ggtgttcaaa
     1321 ttacatgcta agaaaaaaga acccttgaat accaagggtt ctttctcagt ctgataacaa
     1381 tgattaacga cggatttctt tgatacttgt gaaaagtcgt taaagtattg atataaaagg
     1441 atttttatta cctctgacta catattgact aatttgttaa aaaatcgttt ttcaagaatc
     1501 ctgtaatacc agtgctttca ccgttttttt aactctctaa agaactaaaa gcaatcagag
     1561 gttttacctt ttcatttgac gttaagtggc gataaatttt cgaagttgtt tggtcagtat
     1621 ggcctaagcg atctttcaag acttcccagc tacagtgttc cgctactaag cttgccattg
     1681 tatgacgagc taaatgagta tgaaatggct tattaattcc tgagcgattc acgcaccgtt
     1741 tgacgtgatc atttacatac tctcgacgat aaggaacacc acgtttacta gaatcaagat
     1801 acacaaacaa atatgtttct tctataataa acctgtaatg ttgcctcatc tcacccaaag
     1861 ccttaatcaa ctgttgtagc ttttccataa ctagaggagt tactagaact cgtctagcag
     1921 aactaactgt cttagtagtt tcaattcgaa aatccttact cttaccttta actgccgttt
     1981 gcttatagaa cagaatttca tttcttttga aatctatatc tccaggctgt agagcaagcg
     2041 cctcgctgac tcggcagcca gtgtaaaata gaacatggaa aagtacagac atcggtaatt
     2101 cctcatgaac aatgccataa ttcaaaaaag ttctcaattc atcgacagta agaaacttgt
     2161 cctcaatatt atttatggct tcttttaaat cagttaccgt taactgatat tttggcattc
     2221 tcacagactt cattggattt tcacttatca aattctttct ttgacaatat ttaaaaatca
     2281 tatttagaac ttgaataagt gattgtaaac tatttttggc atagccaaca tttgttccat
     2341 taacgccaca ttcctttaag gtaaaaagca attcttgaag gcactctggt gtcaaatcag
     2401 acaacttttt atcaccaatc catcgaacaa taatttttaa ttgttcctct cgcttatcat
     2461 aggttatttc cttttgttca tttaacgaac ggtaatattg gaaccattgc tcacctacaa
     2521 cactaaactt gtcattgctt gcaacaattt ttttcttaag cctttgttgt ttctccattt
     2581 ccagataagc gagttgcgcc tcacgttttg aattaaagcc acgtcttgtt gttcgatatg
     2641 cttgaaagta ccatgtgatc ctaccagtag aactgatttg cttcttgaat cgatctgttt
     2701 tttccattta ttgattcctc ctattttgaa ggaacccaac aagcagaaac cctttggaaa
     2761 tctttctatc tatgtcatca ttgtaccgca tcttagaaag aaaatgtaac ttataaagat
     2821 cgaagtagtc gcaatttttc tcgatagtca ttcgaatcac tctttctcat ccattctaaa
     2881 acatcatcac gaaaatagtg tcctcgaatc ccgtaccaac agggaggaaa ctcaccaagt
     2941 tttactaaac ggttcagtgt actatcactt atctgaaata gtcgctttaa atcagtattt
     3001 gttaatacca gaggtaaatg actgactgca agcaattttt ccgtgtaatc ttccagtttt
     3061 ttttgtaaaa ttgaatagaa ctctatttcc agagaattaa catttgtttt aacttccaac
     3121 tccatcaccc ttctctctca aaatcaaaac gaccaccacg gtacccctta aaattgtaaa
     3181 aaaacactcc agtactccat caaaacggac tagaaaagag gaatttttaa tatgaaacaa
     3241 ggaattgcag acataaaaat aattaaagaa attcttgaaa aatctacggc taatgcaata
     3301 gcattcggaa caggaatcaa cttgagtaca gtaaaaaaac ttaaaagtgg agagagagct
     3361 gaagaaaaat taaatcttgc cgatgctatt aaaatcacag aatttggcat gaaaaatatg
     3421 ccaaccaaaa tcgaaatatg gaaatagtag tttttctgat ctatcagctg ttttgccaaa
     3481 gcacatagga atctcacctc cccccagtaa tggcgggccg atttggttac ggaagtatca
     3541 ttatcgtagt cgcgattctc atattaaaat ttgtcgctta aaagctaacc ttgtcatggc
     3601 gtaactagag aatcaaaatc tacatgtact gatagatttg ctattcattt gtcaaagagc
     3661 gctaggctat tttcagccta tggaaatcct ttattttgac ccgataaaaa acttccatag
     3721 aatgaaaagg aaagggaaaa aatactttaa cgagttgtat caaattcaag tatcttctca
     3781 atcaattttg tttccagttg tctacgcata tattcatcta catacataac cattcctttt
     3841 tcttcttcat aaactggttt catggctaac cgtgaaatat aattttgata ttttgacaga
     3901 acgatggaca tggcaattga atcaccagaa gctgcggctt tgattgtcgt tagtggtatg
     3961 aatggtatat atcgtttatt ttgcttcata tttgacaccc aaatactttc ctagctcttc
     4021 caaagccatt tgtcgttggt accaaacact gccgagactt ttcccaataa tatcgccaat
     4081 ttcacgatca ttaagaccga tgaagaagga cataaaaatg atttcttgtc tttgactagg
     4141 taatgctgca atcgcatttg ccaactcctc attggaaata aagattttca taccaaggac
     4201 aataaaagta ccactactat tcaaatttgg ttccaaataa tagaactctg tacctaaccc
     4261 tttttgttcc aatatttcca ttgatatttg ccgttggcga aatctagcgt acttcttttg
     4321 aatatttaac gcctcattgc agactacttt cttacaaaaa ctgtcaaacg tatgttgaat
     4381 gtgggtttcc tctgagtcgc ccataatatt ctccccctca aaaaattcat tcaactcgtt
     4441 ttacttctat ccctttccat tattactaca atctagga
//
LOCUS       DASHHB010000114         4433 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.121, whole genome shotgun sequence.
ACCESSION   DASHHB010000114 DASHHB010000000
VERSION     DASHHB010000114.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4433)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4433)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4433
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.121"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(543..2048)
                     /locus_tag="U2K71_002748"
     CDS             complement(543..2048)
                     /locus_tag="U2K71_002748"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing histidine kinase"
                     /protein_id="HEM7316755.1"
                     /translation="MIKPKKSEKRELKGPSLTIKWAFASSFFIFVVFTIFAVITYKSS
                     INLIVAKERNNVERTISEVASRLANSDEELTLVNTYRNLTKATNDGDEVVDHTSLVEG
                     NLMKIDSFIAELGQPSMDLYVYDLDENLIFKTHENSRNLIQTTRKVPTIVTLEGKTGF
                     LSIQPIYSKGTREKIGYAQSFYELSSFYDIRNKLLLTLIVLEVVSLILSSILGFFLSS
                     YFLKPLKVLRDTMDTIRKDPQSDIHMPEIDTNDELADLAEIFNEMLDRMRLYIEQQEQ
                     FVEDVSHELRTPVAIIEGHLSLLNRWGKDDPEILEESLSASMQEISRMKSLVQEMLDL
                     SRAEQVDVHYANETTKGKEVTYQVYNNFKILYPDYVITLDDDLTKEVTLQIYRNHFEQ
                     LIIIILDNAVKYSTTRKEVHISISSTLNEFEIAIQDFGEGIPKEDLNKIFNRFYRVDK
                     ARARTKGGNGLGLSIAKQLVENYKGRILAESVVGQGTIFRIFIPIAKAEDK"
     gene            complement(2045..2731)
                     /locus_tag="U2K71_002749"
     CDS             complement(2045..2731)
                     /locus_tag="U2K71_002749"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010763438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HEM7316756.1"
                     /translation="MSNILIIEDEKNLARFVELELKHEGYNAEVHYNGRTGLDAALNN
                     EWDAILLDLMLPELNGLEVCRRVRQVKNTPIIMMTARDSVIDRVSGLDHGADDYIVKP
                     FAIEELLARLRALLRRIDIEGDKNVAKQTTITYRDLTIEKENRVVRRGNEVIELTKRE
                     YELLLTLMENVNVVLARDVLLNKVWGYETEVETNVVDVYIRYLRNKIDVPGEESYIQT
                     VRGTGYVMRS"
     gene            complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U2K71_002750"
     CDS             complement(2924..4345)
                     /gene="gndA"
                     /locus_tag="U2K71_002750"
                     /EC_number="1.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent phosphogluconate dehydrogenase"
                     /protein_id="HEM7316757.1"
                     /translation="MSKQQIGVVGMAVMGKNLALNIESRGYSVALFNRTGAKTTAVVE
                     EHPDKNFKATYTIEEFVESIEKPRRILLMVKAGPATDATIQELLPHLDKGDILIDGGN
                     TFFKDTMRRNEELANSGINFIGTGVSGGEEGALKGPSIMPGGQKEAYALVAPILEQIS
                     AKAEDGAPCVTYIGPNGAGHYVKMVHNGIEYGDMQLIAESYDLMKNILNLSVEEMADI
                     FKEWNQGELDSYLIEITADILTRKDDEGTGKPVVDVILDAAGNKGTGKWTSQSALDLG
                     VPLPLITESVFARYISAYKEERVQASKILSRTNDFEFTGDKKELVEKIREALYFSKIM
                     SYAQGFAQLRVASKEFDWDLPFGEIAKIWRAGCIIRARFLQKITDAYDKNPEIENLLL
                     DDYFVEITKKYQQSVRDVVALAVQAGVPVPTFSSAIAYFDSYRAERLPANIIQAQRDY
                     FGAHTYERVDKEGIFHYSWYHEE"
BASE COUNT         1239 a          919 c          729 g         1546 t
ORIGIN      
        1 tctttttgtt ttaacaactc tttaatctta tcagattcag aagagaatgt caacaacttc
       61 tttgtttatt ttttctaaac gtctttgcca ctcattagca gcgacttgat tatcatacca
      121 tctttattgt tatttgtcaa taactttttc gaagtttttt tcgataaaat caagctttat
      181 tagctatctc atccaaacga cttcgttatt ataacatgac atcaaaagtg cgtcaacact
      241 attttttata atttttagtg acaccttaaa ggaatcattt agaacaaata ataatatagc
      301 aatctcttta taaaattgca atagaaaatt ccctattttt atttattatt tttaaacacg
      361 aatattacag cattttattg ttttttcgtt catctaaaag atacttacat ttaattaacg
      421 gatgttattc tatttaattg ttcgctagtt tttttataat gacatccctg aatttctcac
      481 ttgcttgtat tttgtacctt attctttgtt ggatatataa aaaaacaagt aaagcacatg
      541 ctttacttgt cttctgcctt tgcgatagga ataaagattc taaaaattgt cccttgccct
      601 actacacttt ctgctaagat acgtccttta tagttttcaa ccagttgttt agcgatagac
      661 aaccccagac catttccacc cttcgttcta gctcttgctt tatccactcg ataaaaacga
      721 ttgaagatct tattaaggtc ttctttgggt attccttcgc caaagtcctg aatcgcgatc
      781 tcaaattcat tcagagtgga agaaatagag atatggactt cttttcgtgt agttgaatac
      841 tttactgcgt tatccaatat gataatgatt aattgttcaa agtgattacg ataaatttgc
      901 agggtcactt cttttgtcaa atcatcatct aaagtgatca catagtctgg atataatatc
      961 ttgaaattat tataaacttg ataggtgact tcttttcctt tagttgtttc attcgcataa
     1021 tgaacatcta cttgttctgc tcttgataag tctagcatct cttgaactaa gcttttcata
     1081 cggctgattt cttgcatgct ggcgcttaat gattcttcta atatctcagg atcatctttc
     1141 ccccaacgat ttagcagact aagatgccct tcaataattg caacaggtgt ccttaactca
     1201 tgagatacgt cttccacgaa ttgctcttgt tgttcaatat acaaacgcat acggtcaagc
     1261 atctcattga atatttcagc taaatctgcc aattcatcat ttgtgtcgat ttcaggcatg
     1321 tggatatctg attgcggatc tttacgtata gtatccattg tatctctgag taccttgagt
     1381 ggttttaaga aataagaaga taaaaagaaa cctaaaatac tacttaagat caaagaaaca
     1441 acttctaaaa caataagagt caataatagc ttatttcgaa tatcatagaa agaagataat
     1501 tcatagaagc tttgcgcata gccgatcttt tctcttgtac ctttagaata tataggctgg
     1561 atagataaaa agccagtttt tccttctagt gtaacgatcg ttggaacttt acgtgtagtc
     1621 tgaatcaggt ttcgactgtt ttcatgggtt ttaaaaatca agttctcgtc caaatcataa
     1681 acataaagat ccattgatgg ctgccctagc tcagctataa aggaatcgat cttcattaag
     1741 tttccttcga ccagtgaagt atgatctacg acctcatctc cgtcatttgt cgctttcgtc
     1801 agattacgat acgtatttac taatgtcagt tcttcatccg agttagccaa acgagaagct
     1861 acttctgaaa tcgtacgctc tacattgttt ctttcttttg ccacaatcag attgatagat
     1921 gatttatagg taatgacagc gaaaattgta aacacaacga atatgaagaa agaactcgca
     1981 aaggcccact tgattgtcaa ggagggccct ttcagttctc ttttttccga ttttttcggc
     2041 tttatcacga gcgcatcaca taacctgttc cgcgtacagt ttggatatag ctttcctccc
     2101 cgggaacgtc aattttgttg cgaagataac gaatatatac gtccactacg tttgtttcaa
     2161 cttctgtttc gtatccccac actttattta gtaaaacgtc acgtgctaat accacgttca
     2221 cgttttccat taaagtaagc agcaactcgt attctctttt tgttagttca attacttcat
     2281 ttccgcgacg tacgacacgg ttttcttttt cgatagtcaa atcgcgataa gtaatagtag
     2341 tttgtttagc cacattttta tcgccttcaa tatcgattcg acgcaacaat gcacgaagac
     2401 gtgctaataa ttcttcgata gcaaatggtt tgacgatgta gtcatctgct ccgtggtcaa
     2461 gtccagatac acgatcgatc acagaatctc ttgcagtcat cataatgatt ggtgtatttt
     2521 tgacttggcg tacacgtctg cagacttcta aaccgttcaa ttctgggagc atcagatcca
     2581 acaatatggc atcccactca ttatttaatg ccgcatctaa accagttcgg ccgttgtagt
     2641 ggacttctgc gttataccct tcatgcttca actctagctc gacaaatcgt gccaagttct
     2701 tttcatcttc aatgattaga atattgctca tttattattt tatcctttct attctactgg
     2761 ctttttaaaa actctatcat tataccgata atatgcttaa aaatctatag tcattttatc
     2821 atggatagca attttaaaaa agagctgaag tctactcata acaatcgact tcagctcttt
     2881 cgtcctcttt ttattttctc atttcctaac agccgaaaga ctattattct tcatggtacc
     2941 atgagtaatg gaagattcct tctttgtcga cacgctcata ggtatgtgcg ccaaagtaat
     3001 cgcgttgggc ttggattata tttgcaggca gacgttctgc tctgtatgaa tcgaagtaag
     3061 cgatggctga tgagaaagtc ggtacaggta cgccagcttg gacagccaag gcaacgacgt
     3121 ctctgactga ttgctgatat tttttcgtga tttcaacgaa gtaatcatct agcaataaat
     3181 tttcgatctc aggatttttg tcataggcat ccgtaatttt ttgcaagaat cgagctcgga
     3241 tgatacatcc agcgcgccag atttttgcga tttcaccaaa tggcaagtcc caatcaaatt
     3301 cttttgaagc gacacgtaat tgagcaaatc cttgggcata actcatgatt ttactgaaat
     3361 acaacgcttc tcggattttt tcgaccaatt cttttttgtc tcccgtgaac tcaaaatcgt
     3421 tcgtacgaga caaaatcttg cttgcttgta cgcgttcttc tttataagca gaaatatagc
     3481 gggcaaatac agattcagtg atcaatggaa gtggcacacc taagtcaagc gcgctttggc
     3541 ttgtccattt acctgttcct ttattcccag ctgcatcaag gatcacatct actactggtt
     3601 tacctgttcc ttcatcgtct ttacgtgtca gaatgtcagc agtgatttct atcaaatagc
     3661 tgtctaattc gccttgattc cattctttaa agatatcagc catttcttct actgaaaggt
     3721 ttaaaatatt cttcattaag tcataagatt cagcaatcag ttgcatatct ccatactcaa
     3781 taccgttgtg taccattttc acataatgtc cggctccatt tgggccaata tatgtgacac
     3841 atggtgctcc gtcttctgct tttgcagaaa tttgctctaa gattggtgca actagcgcat
     3901 aggcttcttt ttgtccacca ggcatgattg aaggtccttt taaagctcct tcttcaccac
     3961 cggaaacgcc tgtaccgata aagttgatgc cagagttggc taactcttca ttacgacgca
     4021 tcgtatcttt aaagaatgta tttcctccgt caatcaaaat atcgccttta tcaaggtgag
     4081 gaagtaactc ttggattgtt gcatctgttg ccggtcctgc tttgaccatt agcaagatcc
     4141 gacgtggttt ctcgatagat tctacaaatt cttcgattgt atatgttgct ttgaaatttt
     4201 tatctgggtg ttcttctact acagcggttg ttttagcccc tgtacgattg aataaagcca
     4261 ctgaatagcc gcggctttct atgttcaagg ctagattttt acccataacg gccataccga
     4321 caacgccgat ttgttgtttt gacatatgaa ctcctcctac atacgggatt tctccctaat
     4381 ctcttacatt atagcttaac acggctacat tgcaaactaa agaacaacat att
//
LOCUS       DASHHB010000115         4424 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.122, whole genome shotgun sequence.
ACCESSION   DASHHB010000115 DASHHB010000000
VERSION     DASHHB010000115.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.122"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            690..1658
                     /locus_tag="U2K71_002751"
     CDS             690..1658
                     /locus_tag="U2K71_002751"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HEM7316758.1"
                     /translation="MDVAKTVNAIVEKNDGEISGIYLVACGGSLVDMYVSDYFLKAEA
                     SKCITGLYTANEFNHVTPKQLDDKSVVILCSHGGNTAETVKAGEVAQARGAVTVGLTH
                     NKTADLLKVSDHSFLYEWGDDAKIVNNPMAIILDLTVSLVNVTETFEHYGKFREGMKV
                     IDTVIENGIKQVQDRIKVFADKYKDETMFYILGSGPSYGHAYGFSICSLMEMQWLDST
                     SVHSGEFFHGPFEVTDKDTNFILLMSIGRTRALDERAKRFLDKYAERVEIVDAKELGL
                     DMISDEVSEFFNPILFYSILSEYRSALADVRNHDLDVRRYMGKVEY"
     gene            1669..2484
                     /locus_tag="U2K71_002752"
     CDS             1669..2484
                     /locus_tag="U2K71_002752"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fructoselysine 6-kinase"
                     /protein_id="HEM7316759.1"
                     /translation="MKVLGLGDNVVDKYENLGIMYPGGNSLNFAVFAKKLGHESSFMG
                     VFGSDEEAAHVLSAIKEIGLDNSHSRFEKGENGCARVKIDEGDRIFLGSNSGGVTREY
                     PIQLTEEDREYLNQFELIHMGLYSHVNHLLEELQNVSGKISYDFSDDFTEEEVQRAID
                     KVDFGFFSIEDFSDEEVKQFLMKHFCERNDVLIATRGEKTAIGYDGAKFYEVNPVLEE
                     PVDTMAAGDSFLTAFLLYFLEGDDIVTAMKKGNEFAGKSCLVDGSFGFGIHYD"
     gene            2552..3262
                     /locus_tag="U2K71_002753"
     CDS             2552..3262
                     /locus_tag="U2K71_002753"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HEM7316760.1"
                     /translation="MLDTNAQLPLYLQLKEILKNKINTGEYPENTKIPAESELIESYE
                     VSRITVRKAVEELVEEGYLLKKQGKGTFVNRHKVFRKIEYIMGFSESCKSNGFTPTSI
                     VLERKIIPATEGMAKKLDIAQGDDVLYIQRKRMADNIPIMLENNYFSYERYSFLMEEE
                     LTDSLYEVLKVQGTLAINPGETALEIVTADDQIAKIMEVAVGTPFFFMDTLINDQNNI
                     PIHVGNQYYLGEVYRFYL"
     gene            3298..>4424
                     /locus_tag="U2K71_002754"
     CDS             3298..>4424
                     /locus_tag="U2K71_002754"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002288620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEM7316761.1"
                     /translation="MKQILRSKMIFDGEQPPFKGYIVINEGIIEKVENNWNYSDLLNE
                     NTELIAYDDQFVMPAIHDNHVFFSGYLAMNAGIDLSDTSSANEAVERIKAVMNHKKAD
                     EPIYAHGWDAALWKTDPSEELLNEINHFGPITAIDKNRSHCWMNAIAKQTYGFTEKET
                     SAESRVLLIKEMLANKKLLAEVYAEFEALLLSKGVISMKEIVFDDAEFLSNIPQRKIL
                     SNLYVQAVQEPLQHELLYEYKEKVFPEKVRFGGVKIMVDGVVADRSGDIYGVYQSGVA
                     SPEIDYEAIKAEVAAFNEVEIPCCLTTEGDKAGYKAASILVEFGKRLPEGVFNSISDL
                     EMVTQKTAEKMNEGQVVAEVYPQILGLNQTLEEAYMSTVIK"
BASE COUNT         1490 a          661 c          921 g         1352 t
ORIGIN      
        1 atacaaataa ttcaccaaaa aggtgagtaa tgccattttt gaaaattgag cttgttttat
       61 gtctacatct gtatccgtat ataatccaaa ggattataaa ctcagaagag tctttatttt
      121 ttctgaatat aactactgta tcaaaaacaa tcgattatga gacaaaacaa aatgacctct
      181 tttatttaag aaaggaggtc attttttgta tcataaattg tattaaaatc aatgtatcac
      241 tttttaatca gttttaagac cataaattgc gtactaaaag aaattctgca attcaaaata
      301 aagcatttta agccattttc aaatttaagt gcacccatct acccgtcaaa tcactttatt
      361 ttccactcca gtagtttcct aattcaactg tgtgcaaaaa cgatttcttc taaacaaatc
      421 aagacaaact tataaacctt catcccagca ccataattat ttaaatcata ggcatttttc
      481 gaaattagta tatgtactca agtttcactg cagattattt atatttttta gagaaataaa
      541 tttttcctat tgacaaacac aaagaaagcg gatacaatgc aattatgtca catgacgtta
      601 tatgacgtta tgtgatatct ttacatttaa tcagttactt ttattttgga taaaattaac
      661 atttttaaaa ttttaaggag gaaatgttga tggatgtagc aaaaacggtt aacgcaattg
      721 tagaaaaaaa tgatggagag atttctggaa tttatttggt ggcttgtggc ggttcgctgg
      781 tagatatgta tgtctcagat tattttctta aagccgaagc atcgaagtgt attacaggct
      841 tatatacagc gaacgaattt aaccatgtaa ccccaaagca attagatgat aaatctgttg
      901 tcattctatg ttctcatgga ggaaatacag cagaaacggt aaaagcagga gaagtcgctc
      961 aagctagagg agctgtgact gttggtctga ctcataataa aactgcggat ttattaaagg
     1021 tttctgatca tagtttcctt tacgaatggg gagatgatgc aaaaatagtc aataatccta
     1081 tggccatcat tcttgattta actgtatctt tggtcaatgt taccgaaaca tttgaacatt
     1141 acggtaaatt cagagaaggc atgaaggtta tcgataccgt aattgaaaat ggcattaagc
     1201 aggtgcagga tcgtattaaa gtgtttgctg ataagtacaa agatgaaaca atgttttata
     1261 tcttaggtag tggaccatca tatggacatg cttatggttt ttcaatttgc tctttgatgg
     1321 aaatgcaatg gcttgattca acttctgttc attctggaga attcttccat ggaccttttg
     1381 aagtaacaga caaagatacg aattttattc tcttgatgag cattggacgc actcgtgctt
     1441 tagatgaacg ggcaaaacga ttccttgata aatatgcaga aagagtagaa atcgttgatg
     1501 caaaggaact aggattggat atgatatcag atgaagtttc tgaattcttc aatcccattt
     1561 tattctacag tattctttca gaatatcgtt cagctttagc agatgtacgc aatcatgatt
     1621 tagatgttcg tcgctatatg gggaaagtgg agtactagga ggattaaaat gaaggtatta
     1681 ggcttaggtg ataacgtcgt cgataaatat gaaaatctgg gaattatgta tcctggagga
     1741 aattcattga attttgcagt ttttgcaaaa aaactgggac atgaaagtag ctttatggga
     1801 gtttttggct cagatgagga ggcagctcat gttttatcag caataaaaga aataggactg
     1861 gacaacagtc actctcgctt tgaaaaaggc gagaatggct gtgccagagt gaagattgat
     1921 gagggcgacc ggattttcct tggcagcaat tcgggaggtg ttactcgtga atacccgatt
     1981 cagcttactg aagaagacag agaatattta aaccagtttg aattgattca tatgggatta
     2041 tatagccatg tcaatcatct attagaagag ttacaaaatg tttctggaaa gatttcttat
     2101 gatttttctg acgactttac ggaagaagag gttcagcggg caattgataa agttgatttt
     2161 ggcttctttt ctatagaaga cttttccgat gaagaagtaa agcagttttt gatgaagcat
     2221 ttttgtgagc gaaatgatgt attgattgca acccgaggag aaaaaacggc tattggttat
     2281 gatggagcaa agttttatga agtgaacccc gtgctagaag aaccagttga tacaatggca
     2341 gcgggagatt ctttcttaac agctttccta ctttattttc tcgaagggga cgatatagtc
     2401 accgcgatga aaaaggggaa tgaatttgct gggaagtctt gtctggttga cggttcgttc
     2461 ggctttggta ttcattacga ttgagcttgt tttaaattcc tgcatttgat aacctaaaga
     2521 aagaaatccc ggtaaagtgg aggttaaaaa aatgctggat acaaatgcac aattaccatt
     2581 gtacttacaa ttaaaagaaa tattaaaaaa taaaatcaat acaggggagt atcccgagaa
     2641 tacgaaaatt cccgctgaat cagagctgat agaaagttat gaggtcagcc gaatcacagt
     2701 gagaaaagca gtagaagaac tggtagaaga agggtaccta cttaaaaaac aagggaaagg
     2761 aacttttgtc aaccggcata aagtttttcg taaaattgaa tatattatgg gtttcagtga
     2821 gagttgtaaa tctaatggtt ttacaccgac tagcattgtt ttggaacgca agatcattcc
     2881 agcaactgaa gggatggcaa aaaaactcga tatcgctcaa ggtgatgatg ttttatatat
     2941 ccagagaaag cgaatggcgg ataacatccc cattatgtta gaaaacaatt atttttctta
     3001 tgaaagatat agtttcttaa tggaggaaga gctgactgat tctttgtatg aagtacttaa
     3061 agtccaaggt accttagcca tcaatcctgg ggaaactgca cttgaaattg tcacagctga
     3121 tgaccaaatt gccaaaataa tggaagtagc tgttgggacg ccctttttct ttatggatac
     3181 tttaatcaac gatcaaaaca acataccaat tcatgtaggt aatcaatatt acctgggaga
     3241 agtatatcgt ttttacttat gatttcctgt ttggatttca aggagtagat tttatttatg
     3301 aagcaaatat tgcggtcaaa aatgattttt gatggagagc agccaccttt caaaggttat
     3361 attgtaatca atgaagggat cattgaaaag gtggaaaaca actggaatta cagtgatcta
     3421 ttaaatgaaa acacggaatt aattgcttat gatgatcaat tcgttatgcc agcaattcat
     3481 gataatcatg tatttttcag tggttatttg gctatgaatg ctggcataga tttaagcgat
     3541 acatcttcag ctaacgaggc tgtagagcgt attaaagctg taatgaacca taaaaaagca
     3601 gatgaaccaa tttatgctca tggctgggat gctgccctgt ggaaaactga cccatcagaa
     3661 gagctattga atgaaataaa ccactttggt ccaatcacag cgatcgacaa aaatcgcagc
     3721 cattgttgga tgaatgctat tgctaagcag acttatggtt ttacagaaaa agaaaccagt
     3781 gctgaaagtc gagtattatt aattaaggaa atgctagcaa acaaaaagct tttagcagag
     3841 gtttacgctg aatttgaagc attactgctt tcaaaaggtg ttatatcaat gaaagaaatc
     3901 gtttttgatg acgcagaatt tctttctaat atacctcaga ggaaaatact tagtaaccta
     3961 tatgtgcagg cagtccaaga acctctacag catgaacttc tatatgagta taaggaaaaa
     4021 gttttccctg aaaaggtccg ctttggtgga gtgaagatca tggtagatgg tgttgttgcg
     4081 gatagaagtg gagatattta cggtgtttat cagtcaggag tagcttcacc cgagattgac
     4141 tatgaggcaa ttaaagcaga agttgctgca ttcaatgaag tagagattcc ttgctgtttg
     4201 acaacggaag gagataaagc gggttataaa gctgcgtcaa ttcttgtcga atttggaaag
     4261 cggttacctg aaggcgtttt taattcgatt agtgatttgg aaatggttac tcaaaagaca
     4321 gcagaaaaaa tgaacgaagg acaagtagta gcagaggtat atccacaaat tctcggcttg
     4381 aatcaaacgt tagaagaggc ttacatgtca actgttataa aaaa
//
LOCUS       DASHHB010000116         4305 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.123, whole genome shotgun sequence.
ACCESSION   DASHHB010000116 DASHHB010000000
VERSION     DASHHB010000116.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.123"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            148..1137
                     /locus_tag="U2K71_002755"
     CDS             148..1137
                     /locus_tag="U2K71_002755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEM7316762.1"
                     /translation="MKKKKQDLINGTFREKKKNLKKVVAFSDNRISPKMRELMTGCGS
                     YLEFIATIDKEKKKLVQAHFCKNRFCPLCAWRKARKDSMMLSIMMQAIAEEKQYQFIF
                     MTLTTPNVKAECLNDEIDLFNHALKKMFMRKRVKTSIKGYVRKLEITYNKKRNDYNPH
                     FHLILAVNKSYFTDKDYYIKQSEWLDLWREVTGKTGINPDGTDEITQLDIRKVKGFQQ
                     EKAVQEVAKYSAKDFELTESQEVFDTFYHAMKGRQLITFNGVFKEYKKKYESGDLDRY
                     KKKDENEYFWFLTASWMRKESKYAVDYREMSADERNYFSGMYVDEKSVEGENE"
     gene            1134..1553
                     /locus_tag="U2K71_002756"
     CDS             1134..1553
                     /locus_tag="U2K71_002756"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316763.1"
                     /translation="MTDSTDKMTIKALADELGITKNTVRNYLVKAGYDLARYKKNGII
                     LLDKELIGVARSIYRSKGGQLTGHLPVNSTGSDFPLEKENEFLRDQLAEKDKHISELT
                     EMIRDQQKLTLLQQQKNDQLLLENQELKNRKWWQFWK"
     gene            1532..1870
                     /locus_tag="U2K71_002757"
     CDS             1532..1870
                     /locus_tag="U2K71_002757"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002321903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316764.1"
                     /translation="MVAILEVRCDMDKIDLREETVNRIEVMYERLADYLNLSDEDNYS
                     EMLERMMDVLNSVINADLAKSSVLTNEFVHKHLAQTTDEAEKEFWLKLMSHIMQLNQI
                     SVINSKEKGM"
     gene            1874..2236
                     /locus_tag="U2K71_002758"
     CDS             1874..2236
                     /locus_tag="U2K71_002758"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316765.1"
                     /translation="MGMTLADIKVQEEKLKKEQAKLRQQKKRLIEKENAAMGKALRKR
                     FGFESFADFERWLSSISDDQQHEKSVGNELRADEKSAPSERNYPNESDGIFGGIPNRK
                     SQMSESGFASMFSDVNSR"
     gene            2458..3864
                     /locus_tag="U2K71_002759"
     CDS             2458..3864
                     /locus_tag="U2K71_002759"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016173818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM7316766.1"
                     /translation="MGFSISFKKGTKNTSISHNNRELTDSQKINDWHKHIDFTKSDEN
                     IYLEQINIREKYEELFGEAVEKYNAKQKRADRKIEDYLAKVRKDKKMEPQREFIVQIG
                     TLDDFRTTRDDGSSTGISEQQAEQNRVIANKILVEYFKEFKERNPSLSVYNAVIHNDE
                     VSPHLHLNIVPVAEGYKRGVQKQPSFNKALQQQGLRADKENGRVIWNNFRNQEVESIE
                     RLMADFGWERELVGTNKIKDIHEYKEIMSEISELRQTAVKERESVSDELRYICRQREE
                     NEREKALVASERAKIEEEKKNMLNVKKEMAVIFQKDMVPLESFKRLPDGSYHLSGNDF
                     VDLYQRATRATNAKRNADNMSEQYLQMLEQNTELNEYVAALEEERHLSGRKQLADLQV
                     ENKKLKEENRSLRQRLERLKNAFQKSITRLSIRFGLAEKDMGLSEELEVLEQEKTLFY
                     RQNVSQHKLERSEDEIEW"
BASE COUNT         1497 a          602 c          996 g         1210 t
ORIGIN      
        1 aaaaaaaaag attgcttttt cttccctagt attttatagt tttaattgac tagaaaaaaa
       61 ctaaactaga aaggagagca atctattttt tgttatcggc ttttcagcct tttttgtata
      121 caaaatttgt agattcagta tagcaagatg aaaaagaaaa agcaagattt gattaacgga
      181 acatttcgag aaaaaaagaa aaatttgaaa aaagtggtgg catttagtga taatcgaatt
      241 tctcccaaaa tgcgtgaatt gatgacaggc tgtggttcat atttagaatt tattgcaacg
      301 attgataagg agaaaaagaa attggtccaa gctcactttt gtaaaaatcg tttctgtccg
      361 ttatgtgctt ggcgtaaagc tcgtaaggat tcgatgatgt tgtcgattat gatgcaagct
      421 attgcggaag aaaagcagta tcaatttatt tttatgacgt tgacgacacc gaatgtaaaa
      481 gcggaatgtt tgaacgatga aattgatttg tttaatcatg cattgaaaaa gatgtttatg
      541 cgcaaacggg ttaagacttc gattaaaggc tatgtgcgaa aattggaaat cacgtacaac
      601 aaaaaacgga atgactataa cccgcatttt cacttgattt tggcagtaaa taaatcttat
      661 ttcacggata aagattatta tatcaagcaa tcggaatggt tagatttgtg gcgggaagtg
      721 accggaaaaa cgggaattaa tccggatggt accgacgaaa ttacccagtt ggatattcgc
      781 aaggtcaaag ggttccaaca agaaaaagca gtacaggaag ttgcgaaata ttcggcaaaa
      841 gattttgaac tgaccgaaag tcaggaagtg tttgacacat tctatcatgc aatgaaaggt
      901 cgtcagttga ttacgtttaa cggtgtattc aaagaataca agaaaaaata tgagtcgggt
      961 gaccttgatc gttataagaa aaaagatgag aatgaatatt tttggttttt gactgcttcg
     1021 tggatgagga aagaatcgaa atatgcggtt gattatcgtg agatgagtgc tgatgaaaga
     1081 aattattttt caggaatgta tgtcgatgaa aaatctgttg agggggaaaa tgaatgactg
     1141 attcaacgga taaaatgaca attaaggcgc ttgctgatga actaggaata accaaaaata
     1201 cagtgagaaa ttatctagtc aaagccggat atgatcttgc tcggtacaag aaaaatggaa
     1261 ttatcttgct agacaaagag ttgattggtg ttgctcggtc aatttatcgg tcaaaaggtg
     1321 gtcaattgac tggtcattta ccggtcaata gcacgggcag tgattttcct ttggaaaaag
     1381 agaatgagtt tttgcgtgat caattagctg agaaggacaa acatatttct gaattaacag
     1441 aaatgattcg tgatcaacaa aaattgaccc tgcttcagca acaaaaaaac gatcagttgt
     1501 tactagaaaa tcaagaatta aaaaatcgaa aatggtggca attctggaag tgagatgtga
     1561 catggataaa attgatttga gagaagaaac agtcaataga attgaagtga tgtatgaacg
     1621 gttagctgat tatttgaatc tttcggatga agataattat agtgagatgc ttgaacgaat
     1681 gatggatgtt ctcaactcgg ttatcaatgc tgatctagca aaatcttcag ttttgactaa
     1741 tgaatttgtt cataagcatt tagcgcaaac aactgatgaa gctgaaaaag aattttggtt
     1801 gaaattaatg agtcatatta tgcagttaaa ccaaatttcc gttattaatt ccaaagaaaa
     1861 ggggatgtag tttatgggaa tgactttagc agatattaaa gtgcaagaag aaaaattaaa
     1921 aaaagaacag gcgaaacttc gccaacagaa gaaacgattg attgagaaag aaaatgctgc
     1981 aatgggcaag gctcttcgca agcgttttgg gtttgaatct tttgctgatt ttgagcggtg
     2041 gctatcgtcg atttctgacg atcagcaaca cgaaaaatca gtgggtaatg agttgcgagc
     2101 tgatgaaaaa tcagcaccaa gcgaacggaa ttaccctaac gaatctgacg gaatattcgg
     2161 agggataccg aatagaaaaa gtcagatgag tgagagtgga tttgcgtcaa tgtttagtga
     2221 cgtaaattca cgataagcga atattttgca tgaagcaaaa taaagcgtaa aaggagttga
     2281 gagcgtgaca cgctttcggc tccttttttc gttgtcactt gtgacggcga aaaccctcgg
     2341 agagcgtttg gaattgtgac agtcatgtca caattaggaa aacgttactc tttgacaaag
     2401 agtaacagaa aggtttacac tctttttatt agcgacagcg cgaaggaggg gtttaacttg
     2461 gggtttagta tttcgttcaa aaagggaaca aagaatacaa gcatttctca taataatcgt
     2521 gagttaactg atagccaaaa aatcaatgac tggcataagc atattgattt cacaaaatcg
     2581 gatgaaaata tctatttgga acaaataaat attcgtgaaa aatatgaaga attattcggt
     2641 gaagccgtag aaaaatataa tgcaaagcag aaaagagccg atagaaaaat tgaggactat
     2701 ttagcaaagg ttcgcaagga taaaaaaatg gaaccacagc gagaatttat cgtgcaaatt
     2761 ggtactcttg atgattttcg tactactcgt gatgatggtt cttctactgg catatcggaa
     2821 caacaagcag aacaaaatcg agtgattgcc aacaaaattt tagttgagta tttcaaagaa
     2881 tttaaagaac ggaatcctag tttatccgtc tacaatgctg ttattcataa tgacgaagta
     2941 agtccgcacc tgcatttgaa tattgttcct gttgctgaag ggtataaacg gggtgttcaa
     3001 aagcaaccaa gttttaacaa agccttgcaa cagcagggtt tacgagcaga taaagagaat
     3061 ggtcgagtga tttggaataa ttttaggaat caggaagttg agtcaattga acgtttgatg
     3121 gcagattttg gttgggagcg tgagttagtc ggtactaaca aaatcaaaga tattcatgaa
     3181 tacaaagaaa tcatgagcga aatttccgaa ttgcgtcaaa cggccgtaaa agagcgtgag
     3241 agcgtttctg atgagttgag gtatatttgt aggcaaagag aagaaaacga gcgtgagaag
     3301 gctctggtgg cttctgagag agcgaagatt gaagaagaga agaaaaatat gctgaatgta
     3361 aaaaaagaga tggcggtcat tttccaaaaa gatatggttc ctttagaaag tttcaagagg
     3421 cttcctgatg gttcgtatca tttatctggg aatgattttg ttgatttgta tcaacgagct
     3481 acgagagcaa caaatgccaa gagaaatgct gataacatga gtgagcaata tttacagatg
     3541 cttgagcaaa atacagaatt aaatgagtat gtagctgcat tggaagaaga gcgtcatttg
     3601 tctggtagaa aacaattagc agatttacaa gtggaaaata aaaagctgaa agaagaaaat
     3661 cgctctttac gccagcgttt agagcgatta aaaaacgcat ttcaaaaatc gatcactcgt
     3721 ttgtctatcc gttttgggct tgcagaaaag gatatggggc tttctgagga attagaagta
     3781 ttagagcagg agaaaacctt attttatcgt caaaacgtct cacagcataa attagagcgt
     3841 tctgaagatg agatcgagtg gtagttacga tgtgcagtca acggtaccgg aggcggtttg
     3901 tgccgaggat aaggagttga cggctaaacc actagcctat taagctgaca tttgctaaaa
     3961 aatggcaagt ccattttttg gccaacttag cgttagcgaa gtttgggggc gcccttttta
     4021 aagggtgcgg ttttatgttt gcatgactaa gaatgagtgg gaaaatttaa cagccctatc
     4081 taacaggcgg ttaaatgtac aaaatatgta caccccctta aaaatgcagt ggtcgattga
     4141 tcgaatttta tgtctttggg ttttggggtt ttaaaacgaa aaaaaggggg cgattttcgc
     4201 cctctttttt gatgtttttt cttatcttga tactataggc aactatttct gcgaagaaat
     4261 cggggctata gtatgggtct atcccttgtg gctctaagga agaac
//
LOCUS       DASHHB010000117         4284 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.124, whole genome shotgun sequence.
ACCESSION   DASHHB010000117 DASHHB010000000
VERSION     DASHHB010000117.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4284)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4284)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4284
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.124"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..237
                     /locus_tag="U2K71_002760"
     CDS             <1..237
                     /locus_tag="U2K71_002760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM7316767.1"
                     /translation="IYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDEFFIQSA
                     ASKRNNIPRKSLDYRTPLEVFLSYVSIDDLSNLI"
     gene            complement(490..639)
                     /locus_tag="U2K71_002761"
     CDS             complement(490..639)
                     /locus_tag="U2K71_002761"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293931.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316768.1"
                     /translation="MTFTKTNMQYRVAFDTTSNRFMAIDAQNENNVAFGITIEQAVRA
                     LNESR"
     gene            1101..1313
                     /locus_tag="U2K71_002762"
     CDS             1101..1313
                     /locus_tag="U2K71_002762"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002308849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316769.1"
                     /translation="MVKAEVGRSYICKPIGVSHELIGCIERTYINTAVVYVESYDQRD
                     YQLINESKYRMLVKFCDISAPAELVS"
     gene            complement(1591..3390)
                     /locus_tag="U2K71_002763"
     CDS             complement(1591..3390)
                     /locus_tag="U2K71_002763"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerophosphodiester phosphodiesterase"
                     /protein_id="HEM7316770.1"
                     /translation="MNYLKRSLGNAWDFLKGTQAYFRDVLLMHGFILFICLPLLSSTT
                     RFILHRGAIDYLSYDNIPSIFSQHPGVLLSLIVVLMTILLLVYFEFTFLLMSVFFIKK
                     KEPISLKQLLHLTILQLKKVRPITFLFFLAYFLLILPISGLSFNSDLLSKIKIPAFIM
                     DFIFANRWIIVSSFLLVYIFLGYIGIRLIFALPEMILRDRPFRAAIRESWSLTKSRLL
                     AITGQFIVIGGTILLLSSLGYIVVILAQSMVEQFFPDYALISAVFAMTLLQGILLFNI
                     VMSTVGIFYIIVDFMDDEGFLPEIPKWFIPQAPNLRFSALKNTGLTLFAVFFGIGVCL
                     YNMDYLTSAVQTKPVTVSHRGVSTQNGAQNTLEALEKTSRDYHPDYVEMDVQETKDGH
                     FVVMHDANLRHLTGVNGTPQDLTLKELTNLTVTENGVQAPVCSFDSYLEKAHKLNQKL
                     LIEIKTSKKDSDDLVERFVHKYRKVILENHHILQSLTYQTVSDLKEAEPNFYVGYILP
                     FNIVGPPVTPADFLTMEYSTINRNFINSAHQDGKEVYVWTINDSDAMSRMMFYGVDGI
                     ITDEMGLLNESVKQTDEEITYSDKLLNFVLGVG"
     gene            complement(3514..4110)
                     /locus_tag="U2K71_002764"
     CDS             complement(3514..4110)
                     /locus_tag="U2K71_002764"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010737813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TVP38/TMEM64 family protein"
                     /protein_id="HEM7316771.1"
                     /translation="MNIAASRKLINFISIIGLGLSIALTIYFINLGVFKDLNALRGLV
                     GDSIILGPIIFVFIQILQVVIPIIPGGISTAAGVLIFGPYAGFIYNYIGICIGSIIIF
                     LLGRRYGKPFILSMISDKTYNKYIGWLDNQNRFEKLFALAIFLPVAPDDALCLMAGLT
                     NMSVKRYTLIILIAKPLSIFLYSMALIYGGQYLSGLLG"
BASE COUNT         1529 a          749 c          733 g         1273 t
ORIGIN      
        1 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
       61 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      121 attcaatctg ccgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaaca
      181 cctttggaag tatttttgag ttacgtaagt attgatgatc tgtctaactt aatttgacaa
      241 ttaagattat attaaatatt atacagataa taacggaata ttatcatcga aacagctaac
      301 aaaattgtca taaaaaacga gatggttgat attagaactt gtgtttttgt ttaggtctag
      361 agataaagag agacaatttc caaatatatg taatgaatac aaaaagacgt ttgtcgcgga
      421 ttaccgttac aaacgtcttt tattgatttt ccgagatctc atcagtcgcc atttggaatg
      481 gttcactcct tatctagatt cgtttaatgc tcggactgct tgttcgattg taataccaaa
      541 cgcaacgtta ttttcgtttt gtgcatcaat cgccataaaa cggttagaag ttgtatcaaa
      601 agctacgcgg tattgcatat ttgtttttgt aaacgtcata aaattcatct cctttgtaat
      661 gtaatttagt tcttgaaagt ttcctttaaa ctttcgaact tataccaagt ttagcatcgt
      721 acgttttttg atgcaattaa aaagcattta tcattttcct tatttatttt ttcttaaatg
      781 gtttagctat tttcttttaa tccgataagt agcccaatca tcaaggttac aaatactatt
      841 gcttgaatat aaatcatatg ctcacctctt tataaataac ttagcaatat aaagaaaaaa
      901 acgcacgaac aacataggaa tataaacaaa cgtttttgtc acaacgtcta agatcgtgtt
      961 aaaaaatagt tatctaaaaa gcagaaagat actcaaaata aaaaataaaa aaatggataa
     1021 aaacgttttc aaatacaagg taatttgtta ttataaaata aagaaaaaac aaaataaaaa
     1081 taaagaaaag agggaatgaa atggtaaaag cagaagtagg aagatcttat atatgcaaac
     1141 cgatcggagt atctcatgaa ctaatcggct gtattgaacg tacatatatc aacacggctg
     1201 tcgtgtatgt tgaaagttat gaccaacgag actatcaact tatcaatgaa agcaagtatc
     1261 gcatgctcgt aaaattctgt gatattagcg cacctgctga attggtttct taaacagtat
     1321 ttttgagaat tcgcaagaac tcccttataa tataaataaa gacagaaggg agcgagaaga
     1381 tatgccaaaa agaaaaagtt cagggaaaat gggaattatt cttgcagtga tctctgcaat
     1441 tcctgctatt ttagctattg ttcgtgcgat gaaggaatca agagaaagtt aagcacacat
     1501 tatgagcgtc aaacaaaaaa cgcatatacc tgcggtggca ggttatgcgt tttttgttgt
     1561 ataaagactt ttttcattaa cgatttttga ttagccgact cccaaaacaa aatttagcag
     1621 tttatctgaa taagtgattt cttcatctgt ttgtttcaca ctttcattta gtagtcccat
     1681 ctcatcggtt atgataccat ccacaccata aaacatcatg cgactcatag catctgaatc
     1741 gttgattgtc catacatata cttcttttcc gtcttggtgg gcagaattaa tgaagttacg
     1801 gttgatcgta ctatattcca ttgtcagaaa atcagcaggc gtcacagggg gcccaacaat
     1861 attgaaaggt aagatgtagc ccacataaaa gtttggttct gcttctttta aatctgaaac
     1921 ggtttggtac gtcaaacttt gcaaaatatg atggttctct aagataactt tgcggtattt
     1981 atggacaaat cgttcgacaa ggtcatccga atctttttta gaggtcttga tctcaataag
     2041 taatttttgg tttagcttat gtgctttttc caaataagaa tcaaaagaac aaacaggggc
     2101 ttgcactccg ttttcagtaa ctgtaagatt tgtcaattct ttcaatgtaa gatcctgtgg
     2161 tgttccattt acccctgtta agtgacgaag atttgcatca tgcatcacta cgaaatgacc
     2221 atcttttgtt tcctgaacat ccatctctac ataatcagga tgatagtctc tgcttgtttt
     2281 ttcaagtgct tctaaagtat tttgcgcccc attttgggta ctcacacctc tgtgagagac
     2341 agtcactggt tttgtctgaa cagctgaagt cagataatcc atattgtaaa gacagacgcc
     2401 aataccgaaa aaaactgcaa aaagagtcaa accagtgttt ttcagcgcag agaaacgcaa
     2461 gttgggggct tgtgggataa accacttagg gatctcaggc aagaagcctt cgtcatccat
     2521 gaaatcaaca atgatataaa aaatccctac ggtagacatc acgatattga ataataaaat
     2581 cccttgtaaa agcgtcatag caaacacagc gctgatcaat gcatagtctg ggaaaaattg
     2641 ttcaaccata ctttgtgcca aaataacaac gatatatcct aaagaagaaa ggagtaaaat
     2701 cgtaccgcca atcacaataa attggcccgt gatggccaaa agacgcgatt tggtcaatga
     2761 ccagctttcc cgaatggctg ctcgaaaagg acgatctcgt agaatcattt ccggtaaagc
     2821 aaaaatcagt cgaatcccaa tatagccaag gaaaatataa acgagtaaaa aggaactgac
     2881 gatgatccaa cgattagcaa aaataaaatc cataatgaaa gcggggattt ttatttttga
     2941 cagtagatca gaattaaaac ttaatccgct gatcggtaga atcaataaaa aataagcaag
     3001 gaaaaacaaa aaagtaatgg gtctcacttt cttcagctga agtatcgtca agtgtaagag
     3061 ttgcttcaaa gagattggtt ctttcttttt aataaaaaaa acactcatca aaagaaaagt
     3121 aaattcgaaa taaactaaca gtaaaatcgt catcaaaact acgattaacg agagtagaac
     3181 cccaggatgt tgagaaaaaa ttgaaggaat attgtcataa gacaaatagt caattgcccc
     3241 tcggtgtaaa ataaaacggg tcgtactgct caataagggt aaacaaataa ataaaataaa
     3301 gccatgcatc agcaagacat cacggaaata agcttgtgtt ccttttaaaa agtcccaagc
     3361 attacctaag ctacgtttca aataattcat cctatcacct gctcttcaca ttgtagcgca
     3421 aatctaatga aaaaggaaag ggtgcagtgt gttttgcaga ccctttccca gatttaacat
     3481 tgtcaatcaa tgactaattt tacaaaataa atattatcca agaagaccac ttagatattg
     3541 acctccataa atcaaggcca tactatataa gaaaatcgac agtggtttag caatcaaaat
     3601 gattagtgta tatcgtttca ctgacatgtt tgtcaatcca gccatcaaac ataacgcatc
     3661 gtcaggagca actggaagaa aaatagctag agcaaacagc ttctcaaaac ggttttgatt
     3721 atccagccag ccaatatatt tgttatatgt cttatcgcta atcatggaaa gtatgaatgg
     3781 ctttccataa cgacgcccta gcagaaaaat gataatcgaa ccaatgcaga ttccaatata
     3841 gttatagata aatccagcat aagggccaaa tatcaggaca ccagctgcag tactgatccc
     3901 tccaggaatg attggaataa ctacttgcag gatttggatg aagacaaaaa taatcggtcc
     3961 taaaatgatg gaatcaccta ctaatcctct taacgcattc aaatctttga aaacacctaa
     4021 attaataaaa taaatcgtca gcgcgattga taggcctaat ccgataatgg atatgaaatt
     4081 tatcagtttt cgagaggcag caatattcat tattcatcaa ctccttagag gttctttgtt
     4141 aactatactt tactagaaaa gcaactaact gcgccatttt ttgatatgac agaaaacaaa
     4201 caattttgta agttcatcat aagtataaaa gattgatctc aaaaaaacat ccaactaaag
     4261 acttatttaa taacttgttt tttt
//
LOCUS       DASHHB010000118         4282 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.125, whole genome shotgun sequence.
ACCESSION   DASHHB010000118 DASHHB010000000
VERSION     DASHHB010000118.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4282)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4282)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4282
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.125"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(37..1257)
                     /locus_tag="U2K71_002765"
     CDS             complement(37..1257)
                     /locus_tag="U2K71_002765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015509263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HEM7316772.1"
                     /translation="MKLIGIVGSAAELSYNRKLLTFIQKQYHELFNLEIIEIKDLPLF
                     NQDDDQTNSPAIQQLNRKILMADGVIIATPEHNHTVPAGLKSALEWLSFKIHPLENKP
                     VMVVGASYYDQGTSRAQLHLRQILDAPGVNALVMPGNEFLLGKAKEAFDQEDRLIDPR
                     TVDFLGSTLASFVKFIKVVSTLAGPKKLPPEDLHATGTIATTIPDVDMSDDHWLEKAS
                     EAVGAVSGNTYVKLNRGLLTVDQINYFLDSMPMELTFADNNNQFLYYNLRQEAKEMLA
                     ARVPGQVGNPLANCHPEKSYKNVEWVIQQLRSGATDAVRVHVPKQVPEKYIVHNYQAM
                     HDENGEYAGINEYILDFKPIIEWYLQQTGQQLVGNEDAVSSASVHGGKTDAVTSASTH
                     DETAVDAVSSASVK"
     gene            complement(1277..1888)
                     /locus_tag="U2K71_002766"
     CDS             complement(1277..1888)
                     /locus_tag="U2K71_002766"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002375087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HEM7316773.1"
                     /translation="MKMKFIGLVGTNSTRSTNRELLQFMKNYFVNEAEIEIVEIKELP
                     LFNKPENTELPNVVKEIAGKIEQADGVIISTPEYDHAVPASLINALNWLSYGIFPFVD
                     KPVMITGASYGTLGSSRAQAHLRQILDSPELKARIMPSSEFLLGHSLQAFDEQGRLID
                     EEQVAKLRELFKDFLLFVTITSQLQHAHQANKREAESFSWETI"
     gene            complement(1931..2863)
                     /locus_tag="U2K71_002767"
     CDS             complement(1931..2863)
                     /locus_tag="U2K71_002767"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD:protein FMN transferase"
                     /protein_id="HEM7316774.1"
                     /translation="MNQVIEETKIWQMMGTTITLQVGHEEPSRLLAELGEWLHVYEHR
                     FSAHDATSELMAINQAAGRQAVIVHPELFELIKLGKAHSCARNSYLNIAIGPVVQKWH
                     IGFEDALVPTKAEITELLLLTDPHQIQLDEKMKTVFLTKPGMMIDLGALAKGYIADKL
                     ITHCRERNAAFALVNLGGNIVVFGESPRRSDGAWRIGLRNPKGSREESLAVVKLNNQS
                     LVTSGIYERYLQNGSSVYHHIIDPKTGFPVKNDVASLTILSDSSVDGEIWTTRLFGAS
                     SLEVLKALEEQPGIEGAVVTKENQFLYTKGFEKI"
     gene            complement(3284..4144)
                     /locus_tag="U2K71_002768"
     CDS             complement(3284..4144)
                     /locus_tag="U2K71_002768"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002350223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7316775.1"
                     /translation="MNIEKFITARKAANLSQKEVSEGICTQATLSKFENNGQVPSLKI
                     LIKLCNRINLPLVELFSSMNVINTQLDEQLEKAEFSLITSEYEQLTQILAAIDPKVID
                     ESELIARYYYLKGFEMIFTNAPITDVLFHFDQLLMAETANTLFRLLAYTGIGMVYARE
                     KELEKAEVYFNRVFEKIYTYPIRTTEDIWRVLNIVFHCGVFFAQDGDLETSDALLNYA
                     IDICSDNHLTYYLARAAAQLAKNAQAKNKSKAEVLELIQDALAFAKINKNKKLIDELN
                     HMKKMILLIN"
BASE COUNT         1219 a          861 c          701 g         1501 t
ORIGIN      
        1 tttattcttt atttaaaact ttgcaacaga acctttttat tttacagaag cactcgaaac
       61 tgcatctact gcagtttcat cgtgtgtgct ggcgctggtt actgcatctg tttttccacc
      121 atgaacagat gcacttgaaa ccgcatcttc atttccaaca agttgttgac ccgtttgttg
      181 taaataccac tcaatgattg gtttgaaatc aagaatgtat tcattgattc cagcatactc
      241 tccattttca tcatgcattg cttggtaatt atgcacaata tatttttctg gcacttgttt
      301 tggtacatga acacgtaccg catctgttgc tccgcttcgc agttgttgaa ttacccactc
      361 tacattttta tacgattttt ctgggtgaca gttggctaat ggattcccta cctgaccggg
      421 cacacgagca gctaacatct ctttcgcttc ttggcgcaaa ttataatata aaaattggtt
      481 attattatct gcaaaagtta attccatcgg catcgaatct aaaaagtaat tgatttgatc
      541 cactgtcaat aacccacgat ttaatttgac gtaagtattt ccagaaacgg caccgactgc
      601 ttcagaagct ttttccaacc aatgatcatc tgacatatca acatcgggta ttgtcgtagc
      661 aattgttcct gtcgcatgca aatcttctgg tggaagcttt ttaggccctg ctaaggtaga
      721 cacgacttta atgaatttta caaaacttgc taatgtactg cctaaaaagt caacagttcg
      781 tggatcaatc aagcgatctt cttggtcaaa tgcttctttt gcttttccca ataaaaattc
      841 atttccgggc atcactaatg cattcacgcc tggtgcgtcc aaaatttggc gcaagtgtaa
      901 ttgcgcgcgt gaagttcctt gatcgtagta agaagcaccc acaaccatta caggtttatt
      961 ttcaagtgga tggattttaa atgatagcca ttcaagtgca cttttcaatc ctgctggaac
     1021 cgtatgattg tgttctggcg tagcaataat taccccatca gccattaaga ttttgcgatt
     1081 tagctgttga atagcaggac tatttgtttg atcatcatct tgattgaaca gaggtaaatc
     1141 tttgatttca atgatttcta aattaaatag ttcatgatat tgtttttgga taaatgttaa
     1201 taatttacgg ttatatgaaa gttcagctgc tgatccgacg atacctatta atttcatctt
     1261 tgtttgctcc cctcgattaa atcgtttccc atgaaaagct ttcggcttca cgtttatttg
     1321 cttggtgcgc atgttgcaat tgactagtga ttgtgacaaa gagtaagaaa tctttgaata
     1381 gttcacgtaa ttttgcgact tgttcttcat caatgagtcg tccttgctca tcaaaggctt
     1441 ggagtgaatg acccaataaa aattctgagc ttggcataat tcgtgccttt aactctggtg
     1501 aatccaatat ttggcgcaaa tgtgcttgtg cgcgtgaaga acctaacgtg ccataagacg
     1561 ctccggtaat catcactggt ttatccacaa acggaaaaat accatatgaa agccaattta
     1621 atgcgttaat caatgaagca ggaaccgcat ggtcatactc tggtgtgctg atgattacgc
     1681 catctgcttg ttcaattttt ccagcaattt ctttaacaac atttggtagt tctgtgtttt
     1741 caggtttgtt aaatagtggc agctccttga tttcgacaat ttcgatttct gcctcattta
     1801 caaaataatt tttcataaac tgtaataatt cacggtttgt tgaccgcgtt gaatttgttc
     1861 ctactaatcc aataaatttc atcttcattc tctttccttt ctaacatcga tttctgactg
     1921 ttcgcacgta ctaaattttt tcaaatcctt ttgtatacaa gaattgattc tcttttgtta
     1981 caactgctcc ctcgataccc ggctgttctt caagcgcttt caacacttct aaagaagatg
     2041 ctccaaataa acgtgtagtc cagatttctc catctacaga agaatcagac aaaatcgtca
     2101 ggcttgctac gtcattttta actggaaagc cagttttcgg atcaatgatg tgatgataca
     2161 ctgacgaacc attttgtaga taacgttcgt agattccaga ggtaactaaa gattggttgt
     2221 ttagtttaac caccgcaagc gactcttcac gtgaaccttt aggatttcgc aaaccaatac
     2281 gccaagcacc atccgaacgt cttggtgatt caccaaaaac aacgatattt cctcctaagt
     2341 taactaaagc aaacgctgca tttcgctctc ggcaatgtgt aattaattta tctgcaatgt
     2401 aaccttttgc taacgccccc aagtcaatca tcattcctgg tttggttaaa aaaacggttt
     2461 tcattttttc atctaattga atttggtgtg gatccgttag cagaagtaac tcagttatct
     2521 ctgcttttgt tggaaccaat gcatcttcaa atccaatgtg ccatttttgt acaaccggac
     2581 caatagcaat attcagataa ctatttcttg cacaactatg tgcttttcct aatttgatga
     2641 gctcaaaaag ttcaggatgt acaatcacag cttgtcgtcc agcagcttga ttgatggcca
     2701 ttaattcaga agttgcatca tgtgcgctga agcgatgttc ataaacgtgc aaccattcgc
     2761 ctaactctgc tagtaatcga ctaggctctt catgcccaac ttgtaatgta attgttgtcc
     2821 ccatcatttg ccagattttc gtttcttcga tcacctgatt cattttgtac cttctttact
     2881 tgaactacgg attacatgag taattagttt ttatttttgt tactgttttc acaaccagat
     2941 gatacgcctg ctagatttat ttgtcaaccg attttatata tttttttata taataaggta
     3001 attttaatat aaaattttat attgtaaaaa aacaaataag ggaaacaaac aacacatttt
     3061 tgtgtgttgc ttatttccct ctaactatcg ttttctattt gatggttaac ctacttaaaa
     3121 taaatactgc aacgacaaac ggagatcaag catgctttgt aacgtatttt acgataatta
     3181 cgactttatt ttattactct atcttgctgc catacccgtc tatccctaat catctttggt
     3241 gaattttttc aaagttagca taacaaaaat aagtattttt tccttagtta atcaatagta
     3301 tcattttttt catatgattt aattcatcaa ttaatttttt gtttttatta attttagcaa
     3361 aagctagtgc atcttgaatc aattcgagca cttctgcctt cgacttattt ttagcctgtg
     3421 cgttcttcgc taattgggca gcagctcgtg ctaagtaata agtcaaatgg ttatccgaac
     3481 aaatatcgat tgcgtaattt agtagtgcat cacttgtttc caaatcaccg tcttgcgcaa
     3541 aaaaaacacc acaatgaaag acgatattta acacccgcca gatatcttct gttgtacgaa
     3601 tcggatacgt ataaattttt tcaaacactc gattgaaata aacttctgct ttttccaact
     3661 ctttttctct agcatagacc attccaatac ctgtatatgc caataaacga aacaacgtgt
     3721 tggctgtttc tgccatcaac aactgatcaa aatggaacaa cacatccgta attggtgcat
     3781 tcgtaaaaat catttcaaat ccttttagat agtagtaacg tgcaatcagc tcagactcgt
     3841 caattacctt gggatcaatg gctgctaaaa tctgtgtcaa ttgttcatat tcactcgtaa
     3901 tcagtgaaaa ctctgctttt tctagctgtt catcaagttg tgtattgatc acgttcatcg
     3961 aagaaaaaag ttcaactaac ggaagattga ttcgattaca taatttgatt aaaattttta
     4021 acgaagggac ttgaccattg ttctcaaatt tacttaatgt tgcttgtgta caaattcctt
     4081 cagacacttc tttttgagat aagttggcag cctttcttgc cgtaataaat ttttcaatgt
     4141 tcattatttt ctctccaatc agacctcaca cgaaaatcat ttctgtctcg attttagcat
     4201 gttttctttg ggaatgcttt aggttctgtt gcaaagtttt aaataaagaa taaagtccct
     4261 tacggtatct atgatttaag ct
//
LOCUS       DASHHB010000119         4116 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.126, whole genome shotgun sequence.
ACCESSION   DASHHB010000119 DASHHB010000000
VERSION     DASHHB010000119.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4116)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4116)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4116
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.126"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..85)
                     /locus_tag="U2K71_002769"
     CDS             complement(<1..85)
                     /locus_tag="U2K71_002769"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM7316776.1"
                     /translation="MPRRQRRTYSKEFKQQIVDLYLAGKPCA"
     gene            155..316
                     /locus_tag="U2K71_002770"
     CDS             155..316
                     /locus_tag="U2K71_002770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002608889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HEM7316777.1"
                     /translation="MQDKDKCINCGKCKKVCQMNKDSVENCNHLECIRCGRCKNACPV
                     DAISYGVRK"
     gene            335..1612
                     /locus_tag="U2K71_002771"
     CDS             335..1612
                     /locus_tag="U2K71_002771"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009898531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7316778.1"
                     /translation="MIKNAIAYITRKKNRTLIIFIILTIVLSCLYSCLTIMKSSNEIE
                     KALYESSNSSISITKKDGKYFNVNQFKDIEKLKEIEEKIIQYDGLAKLKDAKVVSGEQ
                     RINREDLSDEFKNVVSLEATNNTKRNILFSSGVFTIKEGKNIEENDKNSIIVHEEFAK
                     QNNLKLGDEVDLELLDIEKSGKIKSHKFKIIGIFSGKKQETYTGLSSDFSENMVFVDY
                     STSQEILNNSENNEIANKILMYSGSAESTDLALNKLKELKIDESKYFVEKDSNAFEES
                     LESVSGIKYIIKVMTYSIMLGGMVVLSLILILWLRERIYEIGIFLSIGTSKIQIIMQF
                     IFELIFISIPSIISSLFLGNVLLKVIVDGFINSEDSMISGGSLINNSSFMSNITTLGQ
                     SYLILISIIVLSVVFASSLMLIKKPKEILSKIS"
     gene            1622..2278
                     /locus_tag="U2K71_002772"
     CDS             1622..2278
                     /locus_tag="U2K71_002772"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004612033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7316779.1"
                     /translation="MDILEIKNVAYSYANSKEKVLSGVNQKFELGKFYAIVGKSGTGK
                     STLLSLLAGLDKPQTGKILFKNEDIQNKGYSNHRKNNISLVFQNYNLIDYLSPIENIR
                     LVNKSADESILFELGLDKKQIKRNVMKLSGGQQQRVAIARALVSDAPIILADEPTGNL
                     DSVTAGEIINILKTLAKDRNKCVIVVTHSKEVADSADIILELSGKKLKKVNKMNLEVE
                     "
     gene            2278..3654
                     /locus_tag="U2K71_002773"
     CDS             2278..3654
                     /locus_tag="U2K71_002773"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017647836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7316780.1"
                     /translation="MIKNAFAYVTRKSLKSLIIILVILSMSTLSLISLSIKDATDRAS
                     KETFANITNSFSMEINRQVNPGTPRGGGNVKGEDIKKISQTDSIDSYVKRINSVADLV
                     DYDIIETQETLANQSPERAKNFKRTVMLTGVNDSTKETKFVSEAYKLVEGKHLENEDK
                     NKILMHKDLAEKNNLKVGDKIKIKSNLFDADNEKGADETVEVEIKGLFDGHNSGGVSA
                     AQELYENTLITDIHTAAKVYGNTEDTAVYQDATFFVKGDKNLDSVIKDLGKLDINWRE
                     YNLIKSSSNYPALQQSISGIYSISNKLFFGSLIFAGVVVSLLLFLWMNARKKEIAVLL
                     SLGISKLEIFGQFLIEMVFISIPAFVGSYFLAQYTADKLGNNILNKVTGDIAKQIAKQ
                     SASSQLGGGAEAEGFNKTLSSLDINVLPKFMIYVVIFMSLVLLVSLIISSFNILRRNP
                     KELLIDNN"
     gene            3784..>4116
                     /locus_tag="U2K71_002774"
     CDS             3784..>4116
                     /locus_tag="U2K71_002774"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEM7316781.1"
                     /translation="MARKKRNPDAEKLAESILNTYQPESVEDMQDVLKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKKHDNRRNGYGNKTLKTSFGKVNIDVPRDREASFEPELIP
                     NRKRDVSDI"
BASE COUNT         1629 a          455 c          727 g         1305 t
ORIGIN      
        1 ctgcgcaagg cttaccagcg agatagagat cgacgatttg ttgtttgaat tcttttgaat
       61 aggttcttcg ttgacgtctt ggcatgaaaa atcctccagt gtgtttttta ttattctaca
      121 caccttattt tttctgtcta gtctagtgta gccgattcag gataaggata agtgtattaa
      181 ttgtggaaaa tgtaaaaaag tttgccaaat gaataaagat tcagttgaga attgcaatca
      241 tctggagtgt atcagatgtg gacgatgtaa aaatgcttgt ccagtggatg caattagtta
      301 tggagtaaga aaatagaaag gagtagttag gaaagtgata aaaaatgcta tagcatatat
      361 tacaagaaag aaaaatagaa ctttaataat ttttatcata ttaactatag ttctttcttg
      421 cttgtattct tgtttaacaa taatgaaatc aagcaatgaa atagaaaagg ctttatatga
      481 aagttctaat tcttcaatat caataacaaa aaaagatggt aaatatttta atgttaatca
      541 atttaaagat attgaaaaat taaaagaaat tgaagaaaaa ataattcaat atgatggatt
      601 agcaaaacta aaagatgcta aagtagttag cggagaacaa agaataaata gagaagattt
      661 atctgacgaa tttaagaatg ttgtttcact cgaagctaca aataatacta aaagaaatat
      721 tttatttagt agtggagtat ttacaattaa agaaggtaaa aatatagaag aaaatgataa
      781 gaattcaatt attgttcatg aagaatttgc taaacaaaac aatctaaaat taggtgatga
      841 agttgatctt gaattactag atattgaaaa aagtggaaaa ataaaaagtc ataaatttaa
      901 aattataggg atcttttctg gtaaaaaaca ggaaacatat acaggattat cttctgattt
      961 tagtgaaaat atggtgtttg tagattattc aacaagccaa gagatattaa acaattcaga
     1021 aaataatgaa attgcaaata aaattttaat gtattctggt agtgcagaat ctacagactt
     1081 agccttaaac aaattgaaag agcttaaaat tgatgagtca aagtattttg ttgaaaaaga
     1141 ttctaatgcg tttgaagagt ctttagagtc agtgagtgga ataaaatata taataaaagt
     1201 aatgacttat tctattatgt tgggtgggat ggttgttctt tcattaatct tgattctatg
     1261 gttaagggaa agaatttatg aaataggtat attcttatct attggaacat ctaagataca
     1321 aattataatg caatttatat tcgagttaat attcatatcg ataccaagta taatatcttc
     1381 cttattttta ggaaatgtat tactaaaagt aattgtagat ggattcatta actcagagga
     1441 ttcaatgatt tccggtggaa gtttaataaa taatagtagt tttatgtcaa atataacaac
     1501 acttggacaa agttatttaa tattaataag tattattgtt ttatcagttg tatttgcttc
     1561 ttcattaatg ttaatcaaga agcctaaaga aatcttatca aaaataagtt aggagaaaat
     1621 aatggatata ttagaaataa agaatgtagc atatagttat gcaaattcta aagaaaaagt
     1681 tttgtcagga gtaaatcaaa aatttgaact tgggaagttt tatgcgatag taggaaagtc
     1741 aggaacagga aaatctacac ttctttcctt acttgctgga cttgataaac ctcaaacagg
     1801 aaaaatattg tttaagaatg aagatataca aaataaagga tatagtaatc atagaaaaaa
     1861 taatatatct ttagtatttc aaaactataa tttaatagat tatttatcac caattgaaaa
     1921 tattagatta gtaaataaat cagcagatga aagtatcttg tttgaactag gtttagataa
     1981 aaaacaaata aaaagaaatg ttatgaaatt atctggtgga cagcaacaaa gagtagctat
     2041 tgctagagca ttggtatcag atgctccaat aatactagct gatgagccta ctggtaatct
     2101 agacagtgtt actgctggag aaataattaa tatattaaag acattagcta aagatagaaa
     2161 taaatgtgta atagttgtaa cacatagtaa ggaagtagca gattctgcgg atatcatttt
     2221 agaactaagt ggtaaaaagt tgaaaaaagt aaataaaatg aatttggagg ttgaataatg
     2281 ataaaaaatg catttgctta tgtaactaga aaaagtttaa aatcattaat tatcatatta
     2341 gttattttat ctatgtcaac tttaagtctc attagtttat ctattaaaga tgctacggac
     2401 agagcttcaa aagaaacatt tgctaatata acaaatagtt tttctatgga gataaataga
     2461 caagtaaatc caggaacacc tagaggtggg ggaaatgtaa aaggtgaaga tattaaaaag
     2521 atatctcaaa cagatagtat agactcctat gtaaaaagaa taaacagtgt tgcagattta
     2581 gttgattatg atattattga aactcaagag actcttgcga atcaatcacc tgaaagagca
     2641 aagaatttta aaagaacagt tatgttaact ggggttaacg actcaacaaa agaaacaaaa
     2701 tttgtatcag aagcatataa attagtagaa ggaaagcatt tagaaaatga agataaaaat
     2761 aaaatcttaa tgcataaaga tttagctgaa aaaaataacc ttaaagttgg agataagata
     2821 aaaataaaat ctaatttatt tgatgctgat aatgagaaag gtgcagatga aacagtagaa
     2881 gtagaaatta aaggtctatt cgatgggcat aatagcggtg gagtaagtgc agcacaagaa
     2941 ctatacgaaa atacattaat cactgatatt catacagctg ctaaagttta tggtaatacc
     3001 gaagatacag ctgtatacca agatgcaaca ttctttgtaa aaggtgataa gaatcttgat
     3061 agtgtaataa aagatcttgg gaaattagat ataaattgga gagaatataa tttgattaaa
     3121 agttcatcaa attaccctgc attacaacaa tctatatcag gtatttactc aatttcaaat
     3181 aaattatttt ttggctcatt aatatttgca ggagttgtag tttcattatt attattctta
     3241 tggatgaatg ctagaaagaa agaaatagca gtattactat cattaggtat atcaaaatta
     3301 gaaatttttg gtcaattcct aattgagatg gtatttatat caatccccgc attcgttggt
     3361 tcctatttct tagctcaata tacagctgac aagcttggga ataatatatt gaataaggtt
     3421 actggtgata tagctaaaca aatagctaaa caatctgcat ctagccaatt aggtggtggg
     3481 gcagaagctg aaggatttaa caagacatta tcaagtttag atatcaatgt attacctaaa
     3541 ttcatgattt atgtagttat atttatgagt ttagtacttc ttgtatcatt gattatttct
     3601 tcattcaata tattaagaag gaatccaaaa gaattattaa ttgacaataa ttaaaatttt
     3661 cggtgctttt tagcaccaaa aatgagagtg taatataaac cgtgtaagta accatcaatc
     3721 ctaggcgggt ggttgcttca cggtttttat ttttctaaaa taagatcaag aggtgattac
     3781 acgatggcta gaaagaaaag aaatcctgat gctgagaagt tagctgaatc cattttgaat
     3841 acttatcaac ccgaatctgt agaggatatg caagatgttt taaaagatgt gtttggtccc
     3901 ttatttgaaa aaatgcttca aggagaattg aataaccatt tagggtatga cgcccattct
     3961 aaagagccaa agaaacatga caatcgacga aacggttatg gcaataagac gcttaaaacg
     4021 agctttggta aagtaaacat tgatgttccc agagaccggg aggcttcttt tgaacctgag
     4081 ttgattccta atagaaaacg agatgtctcc gacatc
//
LOCUS       DASHHB010000120         4075 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.127, whole genome shotgun sequence.
ACCESSION   DASHHB010000120 DASHHB010000000
VERSION     DASHHB010000120.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4075)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4075)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4075
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.127"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            287..694
                     /locus_tag="U2K71_002775"
     CDS             287..694
                     /locus_tag="U2K71_002775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002302104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316782.1"
                     /translation="MSYRVQLIISEDVESQQFGTNVINKVINPALSINAPLIPTALSF
                     SVTAVVSEIEDTEKIKIVEIEVLNKNEKQIFSTGEVSVSLPPQVNDINFNINARNVLV
                     EEAGEHYAVVKFNGTEIGRQIFDIKVNKPVEKN"
     gene            708..1109
                     /locus_tag="U2K71_002776"
     CDS             708..1109
                     /locus_tag="U2K71_002776"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316783.1"
                     /translation="MLDIVPSNTHRSGSLINFAAIFACASVILASPVVVDASSTPKTK
                     NDNQLVITTKNEINTISSNDGKIFDVINTVLKRKAEINIKYDEDLNLYFFVIKTTSEL
                     FSSDYDVLDTLDNVLSDYKYMGKSVVATLGE"
     gene            1111..1482
                     /locus_tag="U2K71_002777"
     CDS             1111..1482
                     /locus_tag="U2K71_002777"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316784.1"
                     /translation="MFDFNEYLSISKQIESDDKYNSKESYRRTAVSRAYYSAFKKSDE
                     YLKENYDIIYNGSSGKGSHQTVWNLFSTVKELNTLGIHNSGYRMLEKRKCADYIPNET
                     ITKTDMALMNREAEKIINKLT"
     gene            complement(1518..1817)
                     /locus_tag="U2K71_002778"
     CDS             complement(1518..1817)
                     /locus_tag="U2K71_002778"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342980.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316785.1"
                     /translation="MLFNETQLWFKFDPSNRFVKDFYKVWDSEVFFLAIEDSLLINLY
                     YSNKNYFKIPAAKTRMKKGVYFLFDIVTDVPDARSDHRRYDYIKYTFVDPERYKD"
     gene            complement(1823..2053)
                     /locus_tag="U2K71_002779"
     CDS             complement(1823..2053)
                     /locus_tag="U2K71_002779"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002378238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316786.1"
                     /translation="MMEEFIRKNISDEYADFYDQSSKKDKFQMDVSILAILAFSENKQ
                     PVTAKKETVFSEGKIKTRYILEVETKFKNRSE"
     gene            2670..3332
                     /locus_tag="U2K71_002780"
     CDS             2670..3332
                     /locus_tag="U2K71_002780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010740290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316787.1"
                     /translation="MKQNDGRIIWKNQSELKLILTINEFIEKHGITSSRQYQKKLSEN
                     PNSAPSMWFINKKYGSWENLLISIGRENTGYGKWARMSEQELLEIVEAFIKCEKITSQ
                     RMYEQKSVGKNIPSLSTIKKMLGDIRPLFKEKNDGSRFTDFELLLELKNEIIRLKLQD
                     DLSMTKFRKLVQSPKLPSVDTIMKRTNKNWEELMAEIGFDYRRIKIYKQRNNLSKTKK
                     TK"
     gene            3411..>4075
                     /locus_tag="U2K71_002781"
     CDS             3411..>4075
                     /locus_tag="U2K71_002781"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioester domain-containing protein"
                     /protein_id="HEM7316788.1"
                     /translation="MKKPRFKNWRLLATLSLLCQTIGGSLGPTIAFADEITHPQTVTV
                     ELDLAHQYAVEGTFSDGRPMSEVTVPHYAVYNGVKQDIFCIEPGVPIYNEFTPGYEKN
                     PLPDMSEKAKLVSVLWKEAGTDVDTHIVAQKMIWQEVNGYTLHSIKRSDGSAVNIAAI
                     ESKINQAIADYQKKPSFHNSTVKTVLGQSTTVTDTNGLNLSEFDEVVENIANIDYRVN
                     GNQL"
BASE COUNT         1465 a          607 c          724 g         1279 t
ORIGIN      
        1 tgaggggaag tacatatagt tgttgatata gttgctaata ttgttagcta atttgttagt
       61 aaaactgtat acgaatttag atagtaaaaa aacagtttgt tccttatata gaggcacttt
      121 tactactgat taaatgtcag atatgcttac aaatatactt ataaatatgc ttacaaaatt
      181 agctaatatt tccgttgaca aagctttgtt actattgtta aactaaattg actagcaaaa
      241 gtgaaacata aatttattaa ataaatgtaa aaattaggag aaatcaatgt cttatagagt
      301 acaattaata atttcagaag atgtagaaag tcagcaattt ggcactaatg ttattaataa
      361 agtaattaat ccagctcttt ctattaatgc gcctttaatt cctactgctt tatcattttc
      421 tgtaacagct gtggtgtctg agatagagga tacagaaaag ataaaaatag tagaaattga
      481 agttttaaac aagaatgaaa aacaaatttt ttctacgggt gaagtatcgg taagcttgcc
      541 tcctcaagtt aacgatataa actttaatat aaacgccaga aatgtcttgg ttgaagaagc
      601 aggagagcat tatgctgttg ttaaatttaa tggaactgag attggaaggc aaatctttga
      661 tatcaaggtc aacaaaccag tggaaaaaaa ttaaggagac gatacggatg cttgatatag
      721 taccatcaaa tacacatcgg agtggtagct taataaattt tgctgcgatt tttgcttgcg
      781 catcggtaat tctagcatct ccagttgttg tggatgcaag ttctactccg aaaactaaaa
      841 atgataatca acttgtaatt actacaaaaa atgagattaa cacaatttca agtaatgatg
      901 gcaaaatttt tgatgtgata aatacggttt taaaaagaaa agctgaaatc aatataaaat
      961 atgatgaaga tttaaacttg tatttctttg ttatcaaaac aacatctgaa ttgttttcct
     1021 cagattacga tgttttggat actctcgata atgtattatc tgactataaa tatatgggga
     1081 aatctgtagt agcaactttg ggggaataaa atgtttgact ttaatgaata tttatctatt
     1141 tccaaacaaa ttgaatcaga tgataaatat aattctaaag aatcttatag aagaactgct
     1201 gtttcaagag catactacag tgcttttaaa aaatctgatg agtatttgaa agaaaattat
     1261 gatattattt ataatggaag tagcggaaaa ggaagccacc aaaccgtttg gaatttgttt
     1321 tcaacagtta aggaattgaa taccttagga atacataata gtgggtatag aatgttagag
     1381 aaaagaaaat gtgctgacta tattcctaat gaaaccatta cgaaaacaga tatggcgctg
     1441 atgaatcgag aagcagaaaa aataataaac aaactcacct aatgaatttc aagcgagttt
     1501 gtttgttatt tttttattta atctttgtat ctttctggat caacgaaagt atactttata
     1561 tagtcataac gccgatgatc gctacgtgcg tccggcacgt cagtcacgat atcaaacaaa
     1621 aagtatacac ccttcttcat tctagttttc gcagcaggaa ttttgaaata gttcttatta
     1681 gaatagtaga gattgattaa taagctatct tcgattgcta aaaagaaaac ttctgaatcc
     1741 cacaccttat aaaaatcttt gacaaatcta ttcgaaggat caaatttaaa ccataattgc
     1801 gtctcattaa aaagcataac cattactccg atctattttt aaacttagtc tctacctcta
     1861 atatatatcg agtttttatt ttgccttcag agaatactgt ttcttttttt gcagttacag
     1921 gttgtttatt ttcggaaaaa gctaatatag ctaaaattga aacatccatc tggaatttat
     1981 cttttttact gctttgatca taaaagtctg catattcatc actgatattt tttctaataa
     2041 attcttccat cataaaaatc acctcaaaac gattatacga acttacgttc ttgttgtaaa
     2101 gcgatatttg aggggcaaaa agggggcaaa aatgcgttta cctttgatat atctcagatt
     2161 gaacatagtg tttattttat gtcggtattg ttgatttata gctgttttga gggtgttaga
     2221 tacataatag aatatagtgg ttttgtttac gtgcattaca cggaaaagct gctcgtatca
     2281 aagaaatcag aagataaaca cggaaaactt ccttggaaat ctgaaatttg tgaaaagtcc
     2341 ttgtatatca agggcttttt cttttttctc tatcacagtt ttaagccatt tttggctgtt
     2401 ttggaccatt tttcaatcat attcccttaa aaattgtcct agattgtccg acattgacta
     2461 tcagtgtcgt caaaataggg caaaatttag tagtcagctt gacgacaaat ataatacgaa
     2521 aaataccaaa acatttaccg aatttagcaa cttttgaacc caagtttctc aaagggtcag
     2581 ctattagcaa aaaagacaaa acatgtactt ttctactaaa aagttgatgt gataggcttc
     2641 tatctaaata actattagga gtttttatcg tgaagcaaaa tgatggacgt atcatttgga
     2701 aaaatcaaag tgagttaaaa ctgattttaa caattaatga attcatcgaa aaacacggaa
     2761 ttacatctag ccgtcaatac cagaaaaagc tatcagaaaa tccaaattct gcaccaagta
     2821 tgtggttcat taacaaaaaa tatggatcat gggagaattt attgattagt ataggacgag
     2881 aaaatactgg gtatggaaaa tgggctagaa tgtcagaaca agaattattg gaaatagtag
     2941 aagcctttat taagtgcgag aagataacct ctcaacgaat gtacgaacaa aaatcagtag
     3001 ggaagaatat tccttcactt agtaccatta aaaagatgtt aggagatata cgtccacttt
     3061 ttaaagaaaa aaatgatgga tctcgattta ctgatttcga actgctgtta gaactaaaaa
     3121 atgaaataat ccgattaaaa ctacaggatg atttatcaat gacaaagttt cgaaaactag
     3181 ttcaatcccc aaaactacca tcagttgata caattatgaa aagaacgaac aaaaattggg
     3241 aggaattgat ggcagagatt ggatttgatt atcgaagaat caaaatttat aagcagagaa
     3301 acaacctatc caagacaaag aaaactaaat agtatcactc taaatcagac gttcaaaatt
     3361 aaaatttgga cgtctttttt attacccaaa aacgaaagga gatttatttt atgaaaaagc
     3421 caagatttaa gaattggcgt ttgctcgcaa cactgagtct attatgccaa acaattggtg
     3481 gatcgcttgg cccaacgatt gcctttgctg atgaaattac tcatccacaa acggtaactg
     3541 tggaattgga tttggctcac cagtatgctg tcgaaggtac atttagcgat ggtcgtccca
     3601 tgtcagaagt taccgttccg cactatgcag tttacaatgg tgtgaagcaa gatattttct
     3661 gtattgagcc aggggtgccg atttacaatg agttcactcc tggttatgag aaaaatccat
     3721 tgcctgatat gtcggagaaa gcgaaattag tttccgtttt atggaaggaa gctggtacgg
     3781 atgtggatac acatattgtg gcccagaaga tgatttggca agaagttaat ggctacacgc
     3841 ttcactcaat taaacgttct gatggtagtg cagtaaatat cgcagccatt gagtcaaaaa
     3901 tcaatcaagc cattgcggat tatcaaaaga aaccaagctt ccataactcc acagtgaaaa
     3961 ccgtgttagg tcaatcgact actgtgacag atacgaacgg cttgaattta tcggagtttg
     4021 atgaagttgt cgagaatatc gcaaatattg attatcgtgt gaacggcaat caact
//
LOCUS       DASHHB010000121         4066 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.128, whole genome shotgun sequence.
ACCESSION   DASHHB010000121 DASHHB010000000
VERSION     DASHHB010000121.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4066)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4066)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4066
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.128"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..1431
                     /locus_tag="U2K71_002782"
     CDS             82..1431
                     /locus_tag="U2K71_002782"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002294242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7316789.1"
                     /translation="MNEEQELQRGLKNRHVQLISIGGAIGTGLFLGSGKSIQLAGPSI
                     LLAYLITGCICFFIMRALGELLLSNTNNHSFLDFVAEYLGKKAAFITGWTYWFCWVSI
                     AMADLTAIGMYVRYWAPGVPQWLPELIALGLLLCLNMVAVSLFGELEFWFALIKVVAI
                     IAFILVGAYMILTHYTTDIGTASITNLWSHGGFFPTGAKGFILAFQMVTFAFAGIELV
                     GLVAGETENPEKVLPEAINNIPIRIILFYLGSLFVIMAIYPWNSLNPDNSPFVEVFSE
                     IGITIAASLINLVVLSAAASACNSAIYSTGRMLRSLAQEGSAPKKFKKLTTHHVPGNA
                     LTFSTIVIFISVILNYVMPSEVFTLVSSIATTCFLFIWSMLVYTHMKYRKSILGKKAH
                     SFKMPLYPFSNYLVFLYMAFVCVVLFLGKDTRIALLLTPVWFLLLLLIYHMKYERKK"
     gene            complement(1559..2149)
                     /locus_tag="U2K71_002783"
     CDS             complement(1559..2149)
                     /locus_tag="U2K71_002783"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1211 domain-containing protein"
                     /protein_id="HEM7316790.1"
                     /translation="MTKSRLEAFTDGVVAIVLRVLVLEIKIPNTSSFHSLWVIRNTLL
                     AYTISFIFVAVIWVNHHRIFQMAERINYRVVWSNIFLLFWLTLCPSVTSWVGRNPEAF
                     WPEMAYVLVYTMWSFSFGILMRQIMKANKPDSHVVRVLSKDRRSLISMMINLCLIAGV
                     FFVPLIGLFGRFFVSGIWIFSYKKADAYYQRLFPGK"
     gene            2296..3648
                     /gene="brnQ"
                     /locus_tag="U2K71_002784"
     CDS             2296..3648
                     /gene="brnQ"
                     /locus_tag="U2K71_002784"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid transport system II
                     carrier protein"
                     /protein_id="HEM7316791.1"
                     /translation="MTKKLSFKDYLFIGSMLFGLFFGAGNLIFPVHLGQEAGAATFWA
                     NLGFLVTGIGLPFLGVIAIGVSKSSGVYELAKRINQTYAMVFTILLYLVIGPFFALPR
                     LATTSFEIGLAPFIDPSRQTLYLAIFSIVFFVLAWWFSRKPTKILDYVGKFLNPAFLV
                     LLGALLLLAFLNPLGSVNQAPIQPNYQEHAFFTGFTQGYNTLDALAALAFGIIIVTTI
                     QNMGVTKPAEIAKDTIKSGAISIILMGIIYTLLAYLGAMSLGSFALSENGGITLAQIA
                     DHYLGTYGSILLAFIVILACLKTGIGLITAFAETFTDLFPQRNYAFFVALASILPCLA
                     ANVGLTNIIQFSLPVLMFIYPLAMTLILLVLVGPLFKQRTAVYRMTTYFTLIASIFDG
                     LNACPETIKQTPIVQNLLYVAESYLPFFKLGMGWIVPAVIGFVIGLIWSFAKKEEVAD
                     "
BASE COUNT         1172 a          744 c          789 g         1361 t
ORIGIN      
        1 ttttggaatc ttagttgtgt ttttcagaaa actctcttaa tatggaagta attaaacttt
       61 tttagttagg atgatttaaa aatgaatgaa gagcaagaac tgcagcgtgg actgaaaaat
      121 agacatgtcc aactgatttc tattggcgga gcgattggaa cggggttgtt tttaggttcc
      181 ggaaagtcta tccagctagc tggcccttcg atattgctcg cctatcttat tactggatgc
      241 atctgttttt tcattatgcg tgcgttaggc gaacttctgt tatcaaacac caataaccat
      301 tcttttctgg attttgttgc agaatatctt ggcaaaaaag cggctttcat cactggctgg
      361 acgtattggt tctgctgggt atcgattgca atggccgatt taacagccat cggaatgtat
      421 gtacgctact gggcgcctgg tgttcctcag tggcttcctg aactgattgc attaggtctt
      481 cttctttgct tgaatatggt ggctgtctca ttgtttggtg agttagagtt ttggtttgca
      541 ttgatcaaag ttgtagcgat cattgccttt atcctcgtag gagcttatat gatcctcacc
      601 cactatacga cagatattgg cacagccagt atcacgaatc tatggtctca tggcggattc
      661 tttccaactg gtgcaaaagg atttatctta gctttccaaa tggtaacctt tgcttttgct
      721 gggatagaat tagttggatt agtcgctgga gaaacagaaa atcctgaaaa agtgttgccg
      781 gaagctatca ataatattcc tatcaggatc atcttatttt acttaggatc tctttttgtc
      841 attatggcaa tctatccatg gaacagcttg aatccagata acagtccatt tgtagaagta
      901 ttttcggaaa ttgggatcac gattgctgct tccttgatca atttagtcgt attaagtgca
      961 gcagcttctg cctgcaacag tgcaatatat agtacaggac gtatgcttcg ttctttagcg
     1021 caagaaggca gtgcccctaa aaaattcaaa aagttaacaa cacatcatgt gccagggaat
     1081 gctttgacat tttctactat cgtcattttc atttctgtta tcttgaatta cgtgatgcct
     1141 agtgaagtat tcacactagt aagcagtatt gccactactt gtttcttgtt tatctggagt
     1201 atgcttgtct acacgcacat gaaatatcgt aaaagtatcc tcggcaaaaa agctcattca
     1261 tttaaaatgc cgttgtaccc atttagtaat tatctggtat ttctttacat ggcctttgtg
     1321 tgtgtcgtct tgttcctagg aaaagatacg agaatcgctt tattactaac acctgtgtgg
     1381 ttcttgcttc tattactaat ctatcatatg aaatatgaac gtaagaagta agaaagtagt
     1441 tgtgacagaa gtggtcagct tcgagaaata agaaggaatt tacgaaaatt gcttcttaaa
     1501 tttttgtgaa atttcggctt atttcttgga gataaaagct tctgtcccag cctcttttct
     1561 atttccccgg aaacaaccgc tgataatagg catctgcttt tttataggaa aaaatccaaa
     1621 taccagatac aaaaaaacgt ccgaataacc cgatcaacgg tacaaaaaag acaccggcaa
     1681 tcaagcataa gttaatcatc atggagataa gacttcgacg gtcctttgac aaaactctca
     1741 cgacatggga atctggttta tttgctttca tgatttgcct catcaagata ccaaaagaaa
     1801 aactccacat cgtgtatact aagacataag ccatctcagg ccaaaaagct tcagggttac
     1861 gcccgaccca actagtaaca gaaggacata aagtcagcca aaagagcaaa aagatattcg
     1921 accagaccac tcggtaatta attcgttctg ccatctgaaa tatacgatga tggttcaccc
     1981 agatcaccgc aacaaatata aagctgatgg tataagctaa taatgtattt cggatgaccc
     2041 ataaagaatg aaagctagat gtattaggga tcttgatctc taatacaaga actctcaaaa
     2101 caatggctac cactccatca gtaaatgctt ctaatctcga ctttgtcacg atgattcctc
     2161 ctcagatgat tcagcgcttt tattttatca cttacgttat taaataacca tcgttttata
     2221 tagggaattt tctgaaaatt gttgcatatt tttcgaaatt ttgttacttt taaaaaaacc
     2281 gtaaaggagt tatgtatgac aaaaaaatta tcatttaaag actatctttt tataggctcc
     2341 atgcttttcg gtctattttt tggtgctgga aatcttatct ttcccgttca tttgggtcaa
     2401 gaagcaggcg ctgctacctt ttgggcgaat ctaggtttct tggttaccgg tatcggtctt
     2461 cccttcttag gggtgattgc cattggtgta tcgaaaagct caggtgtata tgaattagca
     2521 aaacggataa atcaaacata tgcgatggtt tttaccatct tgctttactt agttatcgga
     2581 ccgttttttg ctttacctcg ccttgcaacc acatctttcg agattggttt agcaccattt
     2641 atcgatccta gcagacagac gctatatcta gcaattttca gtattgtatt ttttgtgctg
     2701 gcttggtggt tttcaagaaa accgacaaag atattagatt atgttggaaa gttcctgaat
     2761 cctgcatttt tggttttatt aggtgctttg cttctccttg cttttttgaa tccactaggt
     2821 tctgtaaatc aagcgcctat tcagccaaat tatcaagagc atgctttttt cacagggttt
     2881 actcaaggat ataacacatt agatgcgctt gctgctttag catttgggat catcattgtc
     2941 acaacaatcc aaaacatggg tgtaacaaaa cctgctgaaa tcgctaaaga cacgatcaaa
     3001 tctggtgcga tcagcattat attgatggga atcatttata ctttgctcgc ttatttaggt
     3061 gctatgagct taggtagctt tgctctaagt gaaaatggcg ggatcacgct ggctcagatt
     3121 gctgatcatt atttaggtac ttatggcagt atcttattag catttatcgt tatattagct
     3181 tgcttgaaga ccggtattgg tttgattaca gcttttgcag aaacattcac agatttgttt
     3241 ccgcaaagaa attacgcatt ctttgttgca cttgctagta tccttccttg tttagcggca
     3301 aacgtaggct tgacgaatat cattcaattt tcgttgcctg ttttgatgtt catctatcca
     3361 ttagcaatga cactgatttt actagtgtta gttggtcctt tgtttaagca gcgtacggct
     3421 gtttatcgaa tgactactta ttttacattg attgcttcga tttttgatgg gttgaacgct
     3481 tgcccagaaa ctatcaaaca aacacctatt gttcaaaatc ttttatatgt tgcggaaagc
     3541 tacttgccgt ttttcaaatt ggggatgggg tggatcgtac cagcagttat cggttttgtt
     3601 atcggactga tttggagttt cgctaagaaa gaagaagtag cagattgata aaagaggttg
     3661 tgaaagagac gttcagcctt gagcaataaa aagtaatttt gaaaaatggc ttctcacatt
     3721 tttgcataaa tttgacttaa taccgagaaa ctgacttttg aacaccgttt attgagaggt
     3781 cgtgaaaaag ccgcttagtt tcaagtatta agaaatattt tgtgaaaatg gcttctcaca
     3841 tttttgcata aatttgactt aataccgaga aactggcttt tgaacaccgt ttattgagcg
     3901 tctataaact gacctgagta ttagagaaac aaaacggcaa agtagctcca tatactttgc
     3961 cgttttgttt tgaactaata ccgaagagaa gctgatcaaa caacatttct tttataatat
     4021 agattttaga catatatatg atataaaaat agttttcgca aaaaaa
//
LOCUS       DASHHB010000122         3921 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.130, whole genome shotgun sequence.
ACCESSION   DASHHB010000122 DASHHB010000000
VERSION     DASHHB010000122.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3921)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3921)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3921
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.130"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            7..624
                     /locus_tag="U2K71_002785"
     CDS             7..624
                     /locus_tag="U2K71_002785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316792.1"
                     /translation="MGFQVLFGSLILTIVFLKWKTSNKKSMWSLVGIVLIIFTVTAAT
                     EANYSHKIQSYKGRLVMIKKDVTRYTSSRTGGLYDYAPLDALKYKDHLKKHEVKVGDR
                     WENASHKAYNSKIVYTVEDADEQNITLPVFNYWGTVVKVNGKKSTIENDNGLVSIKGT
                     GKRTIVEVSSTYPTTMILSLLISSITFIFLFTRYLIRCCGEMKID"
     gene            1644..2813
                     /locus_tag="U2K71_002786"
     CDS             1644..2813
                     /locus_tag="U2K71_002786"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEM7316793.1"
                     /translation="MPNFTTEIMETLINKGDLDELFRCHLELAVNSLLQAELTAFLDY
                     EKYDRAGFNSGNSRNGNYSRSFRTEYGELNLVIPRDRNGKFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMSEISELIEKMYGHHYTPQTISNMTKIVSEDIIAFKERSLESRYSVI
                     FMDATHIPLKRQTVSKEAVYIVIGIRLDGTKEVLGFSLAPTESAYVWKEILQDLKDRG
                     LKEVLLVVTDGLSGINDSIHSVYPNAQFQQCCVHISRNIAHKVRVSDRQEICSDFKLV
                     YQASSKEEANNQIRFMIDKWKKQYPRVVKLLMNPAILTFYNFPPSIRRTIYSTNLIEG
                     FNKQLKKYTKRKEQFPNEESLERFLVSQFNNYNQKFLCRVHKGFKEIHDTLESMF"
     gene            3286..3840
                     /locus_tag="U2K71_002787"
     CDS             3286..3840
                     /locus_tag="U2K71_002787"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HEM7316794.1"
                     /translation="MSYNVQPLRTNEEIQDFQFWLRRTSNPERDSFLFLFGINNGLRM
                     SDIIGLKVGDIRGKSKPIIVEHKTGKRKPIFIDNLREEILLYTEGKEENDWLFPSRQQ
                     GRHITRDRVYQIYADVAEKLGRDDIGTHTLRKTFGYHYYKKTRDIATLMFIFNHSSQA
                     ITKRYIGITEDEIGASLRGFKLGG"
BASE COUNT         1471 a          498 c          643 g         1309 t
ORIGIN      
        1 agaaccttgg gttttcaagt gctttttggt tcattaatct taaccatagt gtttttaaag
       61 tggaaaacaa gcaacaaaaa aagtatgtgg agccttgtag gaatagttct tattattttt
      121 actgtgactg cagcaacaga agcaaattat tcgcataaaa ttcagtcgta taaaggtcgt
      181 ttagttatga tcaaaaaaga tgttacacgt tatacatcct caagaactgg aggcctatat
      241 gactatgcac cattagatgc attgaaatat aaagatcatt taaagaaaca tgaagtaaaa
      301 gttggtgatc ggtgggagaa tgcatctcat aaagcatata attccaaaat agtctataca
      361 gtagaagatg ccgatgagca aaatatcaca ttacctgtat tcaattattg gggaacagtt
      421 gtgaaagtaa atgggaaaaa gagcactata gaaaatgaca atggtttagt gtctattaaa
      481 ggtacgggga aaagaactat agttgaggtt tcatctacgt atccaactac gatgattcta
      541 tctttattaa ttagttcaat aacttttata ttcttattta cacgttattt aataagatgc
      601 tgcggtgaaa tgaaaataga ttagatagat attataaata tgttgattct attttcatag
      661 caatggagaa aatacattag caatatataa aagaaggtat caagagtcac agtgaattta
      721 ttccgaaaag tgaaaagagc aaatataaaa tttaatgaaa atatagaact aaataggaaa
      781 acgtagacgt aattttctac gttttccttt tcatatatat taaatattac ggaaaattat
      841 ataaactaat ttagtgtttt gtttgtaatt cctaattttg ctccactttt agataatttt
      901 taggtctatt taactcatca gacattctat gctcattttt ctccaaattt gtttcaactc
      961 tttcgtactt aattttgcgt atttgaaaat tcttctttta gagtactttt attacgatac
     1021 tagtatgata tatatctata gcaaacagtt aaaatcttcc taaaataagc gaatagcatt
     1081 cctaaaacct ataaaatttt tagatgaagc gacaagtgga caattaataa actacttgaa
     1141 catgaaatta tggatcacct ttcaaattta gaaaatggaa ttcttatact tattgcgcaa
     1201 catttattga ttgcaaaatt atgtgacaaa aaaaactagt agaagaaaga ataatggctt
     1261 gataataaag tactacctat taaaggtagt actttattta atttagtata aattaacata
     1321 ctactatatt ttaggtttca tattatataa attaataata tactaccatt tgttggtagt
     1381 ataatttaaa ggtaataagt tattctttta ttactatgtt tgggttttat agcaaagaaa
     1441 gtttaataag agtttggacc tagcaaacaa tattaatgag gtcattaaat cttattatct
     1501 ttagtgatat aacgaaatat ctacacagac aaaatgatag cgtaaaatat tttgtgtaaa
     1561 tagatattta gggtagaaaa aaagaaagtc cattctgtaa aattaaagtt accacaaccg
     1621 aaattttata ggaggacttt ctcatgccca attttactac agaaattatg gaaacactaa
     1681 tcaataaagg tgatttagat gaattatttc gatgtcattt agaattagct gttaattcat
     1741 tattgcaggc tgaattaaca gcttttcttg attatgaaaa atatgatcgt gctgggttca
     1801 attcaggtaa ttcccgtaat gggaattatt cacgttcatt cagaacagag tatggagaat
     1861 taaatttggt aattcctaga gatagaaatg gtaagttttc tcaacaaact ttacctgctt
     1921 ataaaagaac caatgactct ttagaaacca ctattattca gctatttcaa aaaggaatta
     1981 ctatgtctga aatctctgaa ttgattgaaa aaatgtacgg gcatcactat acgccacaaa
     2041 ccatttcaaa tatgactaaa atagtttctg aagatattat tgcctttaaa gaaaggtcct
     2101 tagaatccag atactctgtc atttttatgg atgctactca cattccttta aaaagacaaa
     2161 ctgtttcaaa agaagcagtt tatattgtaa ttggcattcg attagatgga accaaagagg
     2221 ttcttgggtt tagtcttgct ccaactgaat ctgcttatgt ttggaaagag attcttcaag
     2281 acctaaaaga ccgtggttta aaagaggttt tattagtcgt aactgatggt ttaagtggca
     2341 ttaacgatag tattcatagt gtttatccaa atgctcaatt tcaacaatgt tgtgtccata
     2401 tatctagaaa tatcgctcat aaggttcgtg ttagtgatcg acaagaaatc tgtagcgatt
     2461 tcaaattagt ttatcaagca agttcaaaag aagaagcaaa taatcaaata aggtttatga
     2521 tagataaatg gaaaaagcag tatccacgag tggttaaatt gctcatgaat cctgctatat
     2581 taacctttta taatttccct ccgtcaatca gaagaactat ttattcaact aatttgattg
     2641 aaggttttaa caaacaatta aagaaatata ccaaaagaaa agagcaattt cctaatgaag
     2701 aatctctgga gagatttcta gtatctcaat tcaacaacta taatcaaaag tttctatgcc
     2761 gagttcataa aggttttaaa gaaatacatg atacattaga atcaatgttt taacttgatc
     2821 aacagaatgg gcttttccat ttacacaaaa ttattgacgc tcccgacaaa attatgatct
     2881 tgcgtaaagt tataaaatat taataaaaat attttattaa tgaaaagctt ggtagcacgg
     2941 ttattcatat gctaatttat ttcactttat agtaagggga gttcttagta tattgttcaa
     3001 acttaaattt catataattt aattatgtaa attagctatt ctaagaactt ctacggataa
     3061 ctgcccgtct aaaatttaaa taagagctta gaatcttcat ataagtaagg ttaatttttt
     3121 tgtacaaaaa agtcggaata ttctgagtta ctgaaatgat ataaaatcat aaactaaacc
     3181 ccttgatttt cttcactttt aacagagtat atatataagt tcatgattat ggtaaaatat
     3241 atatataatc atgaactcag aatattttta agaagggaaa caatagtgag ctataatgta
     3301 caaccgttaa ggacaaatga agagatacaa gattttcagt tttggctaag aagaacaagt
     3361 aatccagaaa gagatagttt tttatttttg tttggaatca ataatgggtt acgtatgtca
     3421 gatattatag gattaaaagt tggagacata cgaggtaaaa gtaagcctat tattgttgaa
     3481 cataaaacag gtaagcgaaa accgattttt atagataatt tgagagaaga gattttatta
     3541 tatactgaag gtaaagaaga aaatgattgg ttatttccaa gtcggcaaca aggtcgacat
     3601 ataacaaggg atcgtgtcta tcagatatat gcagatgtag cagaaaaatt aggaagagat
     3661 gatataggaa cacatacatt aagaaaaact tttgggtatc actactacaa gaaaactaga
     3721 gacattgcta ccttaatgtt tatttttaat catagctcgc aagcaattac aaaaaggtac
     3781 attggtataa ctgaagatga aataggggca tcgttacgtg gttttaaatt gggaggctaa
     3841 ttgagatatt aggatatatt ttttgtgtat aatgaaaact tagaaggtga gattaatgaa
     3901 gataactgaa ttttcggaag t
//
LOCUS       DASHHB010000123         3872 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.131, whole genome shotgun sequence.
ACCESSION   DASHHB010000123 DASHHB010000000
VERSION     DASHHB010000123.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3872)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3872)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3872
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.131"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..1139)
                     /locus_tag="U2K71_002788"
     CDS             complement(<1..1139)
                     /locus_tag="U2K71_002788"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016922464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="restriction endonuclease subunit S"
                     /protein_id="HEM7316795.1"
                     /translation="MSNDTRPEIRFPGFTEDWEERKLGDMMDVTSVKRIHQSDWTNSG
                     IRFLRARDIVSAAKNEEPSDYLYISEEKYNEYSKNSGKVSQGDLLVTGVGSIGVPLLI
                     TDDNPIYFKDGNIIWFKNEHKIDGNFFYYSFINNKIQKYIRDVAGIGTVGTYTIDSGK
                     KTPISLPTYDEQIKIGSFFKQLDHTIMLHQHELDLLKETKKGFLQKMFPKNGAKVPEV
                     RFPGFTGDWEERKLGEVVVFLNGRAFKQQELLDSGKYRVLRVGNFNTNDRWYYSNLEL
                     EEDKYANKGDLLYLWATSFGPEMWNEERVIYHYHIWKLKFLSKELDKQYLYTWLLTDK
                     EKIKESTNGTTMVHVTKGGIEQRTFQFPPDINEQQKIGAFFKQLD"
     gene            complement(1129..2724)
                     /locus_tag="U2K71_002789"
     CDS             complement(1129..2724)
                     /locus_tag="U2K71_002789"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEM7316796.1"
                     /translation="MGAELNQKLFSAADNLRSKMDASEYKNYLLGLIFYKYLSDRLLE
                     QVVLLADESLEEYDTVSKQTMLYRELLSDEESKEDLIATIVDILGYSISPEYLFNVLA
                     DQAKQATFQLNDLNKAFVQLASTYNQFNGLFDDVDLQSKKLGTDEQQRNVTITEVIKK
                     LNDVEVLGHDGDVIGDAYEFLISQFASEAGKKAGEFYTPHMVSDMMAQIVTLDQKERR
                     FFSVFDPTMGSGSLMLNVRNYLTHPDNVKYHGQELNTTTYNLAKMNLILHGVDAEEMN
                     LRNGDTLNKDWPTDEPYTFDAVVMNPPYSANWSADTTFLDDSRFNRYGKLAPKSKADF
                     AFLLHGFYHLKETGTMAIVLPHGVLFRGAAEGVIRQKLLEDGSIYAVIGMPANLFFGT
                     SIPTTVIVLKKNRQTRDVLFIDASREFVKGKNQNKLSEENIQKILETYAERKDVEKYA
                     HLATFDEIKENDYNLNIPRYVDTFEEEEPIDMVHVGNDIKKIRQEQQVLEKELLEAIS
                     SLQTTPENEAWLQGALEVFKHEQ"
     gene            2849..3634
                     /locus_tag="U2K71_002790"
                     /pseudo
     CDS             2849..3634
                     /locus_tag="U2K71_002790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336730.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SinI family restriction endonuclease"
BASE COUNT         1214 a          747 c          628 g         1283 t
ORIGIN      
        1 tcatctagct gcttgaagaa ggcaccaatt ttttgttgtt catttatatc cggtggaaac
       61 tgaaatgtcc tttgttctat accaccttta gtcacatgta ccattgtcgt tccgttagtt
      121 gattctttaa ttttctcttt atcagtcaat agccaagtat atagatactg tttgtccaat
      181 tccttagata aaaatttaag tttccaaata tgataatggt agattacacg ttcttcattc
      241 cacatttcag gaccgaagct tgttgcccat aaatataaga gatctccttt attagcatac
      301 ttgtcttcct ctaattctaa gtttgaataa taccatctat cattagtatt aaaattacct
      361 actcttaaaa ccctatattt tccagaatca agtaattctt gttgtttaaa agctctacca
      421 ttcaaaaaaa ctaccacttc ccccaacttc cgctcttccc aatctcccgt aaatcctgga
      481 aaacgaactt ctggcacttt tgctccgttt ttagggaaca ttttttgtaa gaatcctttt
      541 ttcgtttcct tcagcaaatc taattcgtgc tggtgaagca tgatggtgtg atcaagttgt
      601 ttgaaaaatg aaccgatctt gatctgttcg tcataagttg gaagtgagat aggtgtcttt
      661 ttaccactat caattgtata ggttccgacg gttccaatgc ctgcaacgtc acggatatac
      721 ttctgaattt tgttatttat gaacgagtag taaaagaagt ttccgtcaat tttatgctca
      781 ttcttgaacc aaatgatgtt tccatcctta aagtaaattg ggttatcatc agtgattaat
      841 aaggggacac ctattgatcc gacaccagtt acaagcaagt ctccttgaga aactttacct
      901 gaatttttcg aatattcatt atatttttct tctgaaatgt aaagataatc ggatggttcc
      961 tcattttttg cagctgagac aatatcacga gcacgtaaaa atctaatacc tgagttagtc
     1021 caatctgatt gatgaattcg cttcacagaa gtaacatcca tcatatctcc taacttacgc
     1081 tcttcccaat cttccgtaaa accaggaaac cggatttctg gtcgtgtatc attgctcatg
     1141 tttgaacacc tccagcgcac cttgtaacca cgcttcattt tcaggtgttg tttgcaggga
     1201 agagatagct tctaaaagtt ctttttctag cacttgctgt tcttgtcgta tcttcttgat
     1261 gtcatttccg acatgaacca tatcaattgg ttcttcttct tcgaaagtat cgacatagcg
     1321 tgggatattt aagttatagt cattttcttt gatttcatca aaggttgcca aatgagcgta
     1381 tttctcgaca tcttttcttt cagcgtaggt ttcgaggatt ttttgaatgt tttcttccga
     1441 taatttattc tgatttttcc ctttaacaaa ttcacgacta gcatcaatga ataatacatc
     1501 tcgagtttga cgattcttct tcaatacaat caccgtcgtt ggaatcgatg taccaaagaa
     1561 caagttcgca ggcataccga tcacagcata aatactgccg tcttctaata atttttgacg
     1621 aatgacccct tctgcagcac cacggaataa aaccccgtgt ggcaagacga tagccatagt
     1681 ccctgtttcc ttcaaatgat agaaaccatg aagaagaaac gcaaaatctg ctttggattt
     1741 tggcgctaat ttcccgtagc gattaaaccg agaatcatct aagaaggtcg tatctgctga
     1801 ccaattagcc gaataaggtg gattcatcac tactgcatca aatgtgtaag gttcatctgt
     1861 tggccagtcc ttattcaacg tatccccatt gcggagattc atttcttcgg catctacccc
     1921 atgtaaaatc aagttcattt ttgctagatt gtatgtcgtt gtatttagtt cttgcccatg
     1981 atatttaacg ttatctggat gagttaagta gttacgtaca tttaacatca aggaacctga
     2041 acccatggtt gggtcaaaga cactaaagaa tcgtcgttct ttttgatcca acgtcacgat
     2101 ttgtgccatc atatcagaaa ccatatgagg cgtgtaaaat tctccggctt tcttccctgc
     2161 ttctgaagca aattgactga tcagaaattc atacgcatcc ccgatcacat ccccatcgtg
     2221 acctaaaact tccacgtcat tcaatttctt gataacttcg gtaatcgtca catttcgttg
     2281 ttgctcatct gttcctagtt tttttgattg gagatccaca tcatcaaata acccattaaa
     2341 ttgattgtag gtagatgcca attgcacaaa ggctttgttt agatcgttca attggaacgt
     2401 tgcttgtttt gcttgatccg ctaagacgtt gaaaagatac tctggtgaga tcgaataccc
     2461 caaaatatca acgattgtgg caatcaggtc ttcttttgat tcttcatctg aaagtaattc
     2521 acgatacaac attgtttgtt tgcttactgt gtcatattcc tctaaggatt catctgctaa
     2581 aagaaccact tgttccaaca aacggtcgga taaatattta tagaaaatca gtcccaataa
     2641 ataattttta tattctgacg catccatttt actacggaga ttatctgccg cactaaataa
     2701 tttttgattt aattctgcac ccaaaaagat acactcctat ctcttattta aagttgctat
     2761 ataccctctt attttagcat actccctttt agaaaaagca aaaaaaaaag aaaaaatctt
     2821 attcttgata acaagctacg tgacgtatat gcctaacaaa aatagaactt ttgaatcaat
     2881 cttaaatgaa ggtttaacca cagaaccggc atccattatt tttgatgaag ttatcgatga
     2941 cgttgaagat agtaagtatc ataaaaattt aaaagaactt ttttattttg cactcaacaa
     3001 tggaccaagt ttgttcactg gtatatcagt acctgatagt gataatccat taattatggc
     3061 aaaaatttac ttaacaaaat ggtgtaataa atacatcagg gataggaata acccagcata
     3121 aaaaaaacct cttaaaacat ttggcgaaaa agatgctgct ttaactacta gagtagcagc
     3181 aaatgctaat attgatgatc aaaaagtatt gaatgactac ttgcgtggac attttttata
     3241 tatgagcgct gagaacatga acggaagtat tttagaggaa tatttagccg aagtgttaga
     3301 acctgaaggt tggatttggt gcgccggctc tgtatatcga gctgttgatt tctgttattt
     3361 aggaacctca ccaattcttt tacaagtgaa aaataaatac aatacagaaa gcagttcgag
     3421 ttcagctata agagtaggaa caacaattag aaaatggaat cgtttaaata agtcgacaaa
     3481 aatttctggt ttggatagcc ctatccccaa ttggaaagca ttaatagaaa tgactgaagc
     3541 tagtaaggaa ctggcagcta agttaactga aaatagttat ttagcttata ttaatgaaaa
     3601 atccacacga gaattatgga cactcgatga ctaacaatat ccttattagt atctatccac
     3661 gatacatgga aaaaattata aacaaacaaa aaaactttga atttagaaat ttctatctta
     3721 cctcagatcc ttataatatc ttttggattt acgaaacaat ccccaccaaa gctttaaagt
     3781 ataaattgat tgtaaaaaat ccaattacta aacttaagaa aaatcaacac tactttttag
     3841 gagacgatga gtttgaacaa ttagtttaaa ag
//
LOCUS       DASHHB010000124         3862 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.132, whole genome shotgun sequence.
ACCESSION   DASHHB010000124 DASHHB010000000
VERSION     DASHHB010000124.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.132"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(247..468)
                     /locus_tag="U2K71_002791"
     CDS             complement(247..468)
                     /locus_tag="U2K71_002791"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316797.1"
                     /translation="MEEEKIFEKRWELASVEQRARYHNLMSSYRNIDWTYKEKKYLLW
                     LCQLDVNTFETFEVILDKIKNSNEKRADL"
     gene            complement(566..811)
                     /locus_tag="U2K71_002792"
     CDS             complement(566..811)
                     /locus_tag="U2K71_002792"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008271463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316798.1"
                     /translation="MDFPEVVKEVQEIGATISDSRSSPMNYQDAHGIKIIDQERNVRK
                     YTTINECVINENLCRAPITKHIKNRSKAKDGRTFTTF"
     gene            1051..1665
                     /locus_tag="U2K71_002793"
                     /pseudo
     CDS             1051..1665
                     /locus_tag="U2K71_002793"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337511.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
     gene            complement(2035..3036)
                     /locus_tag="U2K71_002794"
     CDS             complement(2035..3036)
                     /locus_tag="U2K71_002794"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4062 domain-containing protein"
                     /protein_id="HEM7316799.1"
                     /translation="MKTINLIKVFIASPGDIAAKRNEVEETIYRWNIENTDNTKVILM
                     PIRWETNAIPTYINNSSGQSVINKQIVENSDILLAIFGHKLGSPVDNYRSGTIAEIDY
                     FYKNSKGEVGVFFLDEPVPQVLAEEYIEVKKYRDSLEDKGFYGTYSKDNIRRFLTKKV
                     NYLIEKAQNNLESKRFDLDSISYKTPSVINKKVTGTDKDFDDNLDIFDPIEFDNDERL
                     LLIYAFMDENSIFSTDNSTYRKVVQWESDNNLRSYLSDRVFDVLEKLSKSGILKEEYD
                     SSFDEYPSRYILISEDYKKFKRYFLKNEKKISVIMEKYKAPIQADPFSNLDESDLPF"
     gene            complement(3195..3461)
                     /locus_tag="U2K71_002795"
     CDS             complement(3195..3461)
                     /locus_tag="U2K71_002795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7316800.1"
                     /translation="MMIKKLIEEKQKDKDFAEAYDREGRKLETAVALYHAREEAGLTQ
                     AELAQKAHTTQSTIARIERGDNVSFDKLDDIARALGKKLVVEFA"
     gene            complement(3451..3807)
                     /locus_tag="U2K71_002796"
     CDS             complement(3451..3807)
                     /locus_tag="U2K71_002796"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM7316801.1"
                     /translation="MEKISFETFKRPNGHDEFLEWLETLPKKDSAKLLRTIEETEKNG
                     MLVAQRLKWVKKLDTNLYELRSKVGSNIQRAIYFHVVNGRYVITHGFTKKTEKTPISE
                     LKHAKELREEWFTNDD"
BASE COUNT         1221 a          698 c          512 g         1431 t
ORIGIN      
        1 ttttactata tacttcttcc cagaacttgt ccgtctcctt caaattatct ctacctctcg
       61 agttcattgg gtggtttgcc catttattaa acatggaatc acctttctca tactcatata
      121 tttatgtcct atttcaatta tatttatata tgcttaaata aatttgtatc ctgaaattta
      181 ttgtaatatg aagctgtatt ttacatatag taaccaaaga gttaataaaa taaaaagagc
      241 gaaccattat agatcagctc ttttttcatt gctgttttta atcttatcta gaatcacttc
      301 aaatgtttca aaggtattta catctaactg acataaccaa agcaagtact tcttttcttt
      361 gtaagtccaa tcaatattac ggtaagagga cattagatta tggtaccttg ctctttgttc
      421 aactgaagcc aattcccagc gtttttcaaa tattttttct tcttccatta ttgatccctc
      481 gcaagacgct acctcaaact taaataatgg gcagcgaaag tctaattcct tagatctaag
      541 tatagcaaac aaatatgaaa aaaacttaga aggtagtaaa agttcttccg tcttttgctt
      601 tgcttctatt cttaatgtgt ttcgttatcg gtgccctaca aagattttca ttaattacac
      661 actcatttat tgttgtgtat tttctgacat ttctctcttg atcaattatt ttaattccat
      721 gggcatcctg atagttcata ggagaggagc ggctatcaga aatagttgca cctatctctt
      781 gaacttcttt tacaacttct ggaaaatcca tccatcctgc ttcagctgtc aaaagtaata
      841 atctataata tttatctttc cttttcaatc taagaaatcg tcaccccatt ctttttcttc
      901 tattataaac tatttaactt ttagaattca aaaaccgttt gtctatttag agtttaccct
      961 aaatagacaa aaatataaca ttgaaaaatt tagagttcta acgtatgatt agggtgtatt
     1021 ataaacgtta tagaaaagta gggaataaat ttgaaaattg cttatattag agtttcatcc
     1081 atcgatcaaa atgaacaacg tcaaattgaa gaaatgaaaa agtttggcgc tgaaaaaatt
     1141 tttatcgaaa aacaatctgg agcgacgatt agtcatcggc cgattttcca ggaagcttta
     1201 gattttgtca gagagcaaga tatttttatt gtagaagcca ttgatcgact tggacgcaat
     1261 tatgacgaga ttatcgattc agtgaattat ctaaagaaga agaatgtgcg acttattatc
     1321 accagtttac cgatcatggc tgaagcaatt ggaaatcctc tcctagataa attcatcaaa
     1381 gacttaatca tttaaatctc agccatgatt gctgaacaag aacgtactga atcgaaacga
     1441 cgccaggcac aaggaattaa aatcgctaaa gcaaacggcg tctataaagg tcgtcctaag
     1501 ttatacagtg cagacaccaa agatcctcaa cgtcgccttg tctataggag tatcgttcag
     1561 gatcttgaga atggggttgc tatctcaaag attgcaacag actacaatgt gactaggcaa
     1621 acaatttacc gaataaaaaa agaaatcgac caactaattg tatgatttct tttttgaatc
     1681 acgtaattat taattagctt tgtcatcaat acagacaatc attttagatt catctagcta
     1741 agttgattac atttatgtta aaaagaattt agttaacata aatataattg atgcataaat
     1801 cgtccttatt taaaggcatt taacattgac aacttagtat aatgtatctt atgtaaacta
     1861 ataagatttg aattttattt tatacacact cgataataga tcaattttat ggtcccccgc
     1921 atttacgatt gcacaagaga aacataaagt cgacctatta gcatttcttt tcaattcaaa
     1981 cattaaaaaa gtttatacct ctgctactaa aaaatgaaat tatctattta aaatttaaaa
     2041 cggtaaatct gattcatcca aattggaaaa tggatcagcc tgtattggag ctttgtattt
     2101 ctccataata acagatatct ttttttcatt ttttaaaaaa taccttttaa atttcttata
     2161 gtcctctgaa atcaaaatat agcgtgatgg atactcatca aaagaagaat cgtattcttc
     2221 ctttaaaatt cccgatttac ttaatttttc tagtacatca aatactctat cactcaagta
     2281 actccttaaa ttgttatcac tttcccattg aactactttt ctataagttg aattatcagt
     2341 tgaaaagata gaattttcat ccataaacgc atatattagc agcaatcttt catcattgtc
     2401 aaattctatt ggatcaaaaa tatctaagtt gtcatcaaaa tctttatcag ttccggtaac
     2461 ttttttgtta attactgaag gtgtcttgta agaaatagaa tctaaatcaa agcgttttga
     2521 ttctaaatta ttttgtgctt tctcaattaa ataattcact ttttttgtga gaaaccttct
     2581 gatattgtct ttgctatatg ttccataaaa acccttatct tctaaagagt ccctgtactt
     2641 cttaacttct atatactctt cagccaaaac ttgtggaact ggttcatcta agaaaaaaac
     2701 ccctacctcg cctttgctat ttttataaaa gtaatcaatt tctgctatag ttcccgatct
     2761 ataattatca actggagaac ccaacttatg gccaaaaatc gctaataata tatcactatt
     2821 ttctacaatt tgtttattaa taacactttg cccagagcta ttatttatat aagtaggaat
     2881 cgcattcgtt tcccatcgaa ttggcatcaa aattactttt gtattatctg tattttctat
     2941 attccatcta tatatggttt cttctacttc attcctcttt gctgctatat cacctggtga
     3001 tgctataaat acttttatta gatttatggt tttcatttat tgtacctttc tctttatgtc
     3061 tctttaaagt aatttttgta taaaatttat tgacttttac actataggac aacttttatc
     3121 tatttagtac aaaaaggccc actaaactaa ttgattagtt aggagcctat tacaggtata
     3181 ttttcaactc gcttttaagc aaattctact accagctttt tgcctaatgc cctagcgata
     3241 tcatcaagtt tatcgaaaga tacgttatcc cctctctcaa ttcgagcaat agtagattga
     3301 gttgtatgtg ctttttgtgc aagctccgct tgagttaggc ctgcttcctc ccgtgcatga
     3361 taaagagcaa ccgcagtttc gagttttctt ccttcacggt catatgcttc tgcaaaatct
     3421 ttgtcttttt gcttttcttc aatcaatttt ttaatcatca tttgtgaacc actcctctct
     3481 aagttctttt gcatgtttaa gttcacttat tggtgttttc tccgtttttt tagtaaatcc
     3541 atgggtaatg acataccttc cattgaccac atggaaatag atagctcttt ggatgtttga
     3601 tcctacctta cttcttaatt cgtacaaatt agtgtctaat tttttcaccc atttcaaccg
     3661 ttgagctact agcatcccat tcttttctgt ttcttctatt gtacgtaaaa gcttcgcact
     3721 atcttttttt ggtaatgttt ccaaccattc taaaaattca tcatgaccat taggtctttt
     3781 aaatgtttca aaagatattt tttccatacc caattatatc atatattgat tttatattat
     3841 agtatataac ataaaaattc ga
//
LOCUS       DASHHB010000125         3763 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.133, whole genome shotgun sequence.
ACCESSION   DASHHB010000125 DASHHB010000000
VERSION     DASHHB010000125.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3763)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3763)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3763
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.133"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..218)
                     /locus_tag="U2K71_002797"
     CDS             complement(<1..218)
                     /locus_tag="U2K71_002797"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5406 family protein"
                     /protein_id="HEM7316802.1"
                     /translation="MKNYDPNIRLGTHTIKVSFQRWDYKGFVTFRRGGNCKGLDVLAL
                     DEDDLYYQKLTDNPIGFGLLPEDDEGNE"
     gene            complement(837..1169)
                     /locus_tag="U2K71_002798"
     CDS             complement(837..1169)
                     /locus_tag="U2K71_002798"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein TraE"
                     /protein_id="HEM7316803.1"
                     /translation="MSYTGILSLEDICHYGKRCTATEKITKKLSTGQSKTVVQCKKYI
                     IQKDKVSEEMIYYAGKQKQIILKDPIPLKELYPTIKHVYDQNGVLIGRRKNGVLRCTA
                     KGMGRLIS"
     gene            complement(2016..3056)
                     /locus_tag="U2K71_002799"
     CDS             complement(2016..3056)
                     /locus_tag="U2K71_002799"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002310953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiator protein A"
                     /protein_id="HEM7316804.1"
                     /translation="MTDFNFFKADRVYNELFYQFPKVFIVSDEYKKMKDSTKIAYMLL
                     KARLEIAISKRQIDEEGNVYFTYTTNELCSVLNCQKQKAIAIKKELESFGLLLQKQMG
                     FNKQLGKNNPNRLYLAELKVSENDIYLLEKFDRENRENVDKSEGMKIIPTLDEKSDAE
                     SLGAQEGMKIIPCQNVDKSEGMKIIPELNNNILDTNRHNIDTEKDRLQDQLLLDNFET
                     IMTNDSIATFIPERVLNLIKTFSSSYSEAQKTVQTIHNAKKKAETESGISIIFEELDS
                     YYVNAEQELYTTLLKAYQKLKTEKVENIQNLIFVYVKNWFIEKPIAAKVSSEKRLNSE
                     SFPKTITKDWLE"
BASE COUNT         1232 a          663 c          496 g         1372 t
ORIGIN      
        1 cattcattac cttcatcatc ttcaggtagc aagccgaatc caattggatt atcagtcaat
       61 ttttgatagt ataaatcatc ctcgtctaat gctaaaacat ctaatccttt acaatttccg
      121 cctcttctaa acgtcacaaa tcctttgtaa tcccaacgtt ggaaagaaac cttaatagta
      181 tgtgtgccca atcgtatatt cggatcataa tttttcattt gtcattcctc cattacgtaa
      241 atttcatttt ctctatgttt tatatcaatt ttttcacttt attcaggact cgttcatatt
      301 ttatcgtttt tcgtttactt aaaaatacat aaaaaatatc gactcgttta tggcgtccga
      361 aacgagaaac cctagtagaa atcggtaaat gctcgattga tccaatgacc aacgtcctcg
      421 caagtatcta catgtactgc agcacacgac acaatttctt gtgcgtcctc cccattacga
      481 gaagacctac tccttattcc atgagtttgc gtcaaagcct agatccctta gtagctgctc
      541 atgattaatc taggcgctat tctaacttgt ctaacttcta aattccctct tgtttactct
      601 gtgcatatta tttacacaaa ttcatttcac cactatccaa acaagggcat ttactcgatt
      661 gaccaatttt ttacgttacc aatcgtaaaa acctaccccg tcaccccccg aaaggaattg
      721 ttgatagatc aagtctactc cttatttttc tagtaattct aagttctttt agaagtctcg
      781 acaatatttt caagcaagcg aaaaacgata aaatacaagt tatgagaatg atcaattcag
      841 ctgattaaac gccccatccc ttttgcggta caacgcaaga ctccattttt tcttcgacca
      901 atcagtacac cgttttggtc ataaacatgt ttgattgttg gatatagttc ttttaaaggg
      961 ataggatctt ttaaaattat ttgcttttgt ttaccagcgt aataaatcat ttcctctgag
     1021 actttgtctt tctggattat atatttctta cactgcacaa cggttttact ttggccagtt
     1081 gatagtttct tcgtgatttt ctctgtagcg gtacaccgtt taccgtagtg acaaatatct
     1141 tcgagactca aaattcctgt atatgacata aaataatcca cccacttact ttaaatattt
     1201 ttaataagtt tttcttgtta tttttcaaaa atagagaatt gattttcgtt catgacaaac
     1261 aaaaaagagg gaacgcatcc aaacatgcgt tccctctaat ttcagcgttc gtcatacggc
     1321 aaagagccac acggacattt ttataacatt atgcttaggc atagattcat cgtaatatca
     1381 cgtattttta accttaacac agccaaaaaa agtggtatca cttttgaata tctgttatac
     1441 taaatccgtt gcgggtaata ccgtaacact gaaaggtaaa cgcattaagg gattaaaggt
     1501 tgtcttaatg ccacctgtgg acgtcgacga aggttaccag ccttccgagt ctcacagggc
     1561 ctttcttttt tttgtatttt caattgtcca aatttatcgt ccaaaagtgc agaacgaaaa
     1621 ttaattcatc acaagcgtag cagacaatta aatttaaaac aactaaattt atacaaaaaa
     1681 atgtaagcga ataatattcg tattgcctga aaagaagtaa tatcaaggtt tttagctttt
     1741 ttaaaaaatg aaattacctc tcttcgcgta ttattataac taaaaaacag caacatgcaa
     1801 acgtttgttc tcttttttgt gttggaaatt aattattaac tattaacact tatgacgtaa
     1861 aaagctacgt ttgtgatttt tatcataata ttttaactca taaattctat taaaatagct
     1921 atttctttgt aacatttgta atataattag aataataaaa aatttttaat tttaatgcta
     1981 ctccatgaag caacaatcaa tttgtcagtg aaatttcact ctaaccagtc tttcgtaata
     2041 gtttttggga agctttcaga attcaaacgt ttttcacttg atactttagc agctattggt
     2101 ttttcgataa accaattttt tacatagaca aaaatcaggt tctggatatt ttcaactttt
     2161 tcggttttta atttttgata ggcttttaac agtgtcgtgt ataattcttg ttctgcattg
     2221 acataataac tatcgagttc ttcaaaaatt attgaaatac cactttctgt ttcggctttt
     2281 ttctttgcat tatgaatagt ctggacagtt ttttgagctt cactgtaact tgaagaaaat
     2341 gtttttatta aatttaatac tcgttcaggg ataaacgtag caatgctgtc gtttgtcata
     2401 attgtctcaa aattgtctaa caacaattga tcttgtaggc ggtctttttc agtgtctata
     2461 ttgtgtctat tagtgtctaa tatattatta ttaagttctg gtatgatttt cataccctct
     2521 gatttatcaa cgttttggca cggtatgatt ttcataccct cttgagcccc aagggattca
     2581 gcgtctgatt tttcgtcgag ggtgggtatg attttcatac cctctgattt atcaacgttt
     2641 tccctattct ctctatcaaa tttttcgagt aagtagatat cattttctga gacttttaat
     2701 tctgctagat atagtctatt aggattattt ttccctaact gtttgttaaa tcccatctgc
     2761 ttttgtaata ataaaccaaa ggattccaac tcttttttga ttgctatcgc tttttgtttt
     2821 tggcagttta atacgctaca tagttcattt gtcgtataag taaaataaac attaccttct
     2881 tcatcgattt gccgtttgct gattgcgatc tctaatcttg cttttaaaag catataggca
     2941 atcttagttg aatctttcat ttttttgtat tcgtcagaaa caataaagac ttttggaaat
     3001 tgataaaata attcgttgta aactctatcc gccttaaaaa aattaaaatc agtcattgta
     3061 gatcctctac tttcacgttg ttttatttat gtaaaattac ctactctttt cgaaaatagc
     3121 aaagataaat aaaaaatgca aacgcattaa aactgttttt taattaaata ctacctaaaa
     3181 tcagaaataa aatgaattat gtactacctt taaagtcatt aagaactact ttaaataaat
     3241 attatttata attaaatact accagaagaa cgtatgaata aaatttagtt ttaaaattaa
     3301 agataattaa gtactaccag tgtcaaaaca aaaaataaat taagaactac cttatgcata
     3361 taaaacatac taccattact agtaatatat actaccttat ttcagtagta tatattatat
     3421 tcttcttata agacaaggct ttaggctaga aaacaataga ttatatacta ctaaaaaata
     3481 caataagtac taccaatgtc agatatatat aataattatg tactaccatt aaaattaact
     3541 tatttaataa gatactactg tgttaatctg aaaaaaatta ctttaaagta gttacatact
     3601 acctgttaaa ttagatacta ccattaatac ttaaattaga tactacctat atttatatta
     3661 agtactaccg ttctaggaat aagataataa aaaaaaacta ccaaattgca attaaatact
     3721 acttaaaatg taaaaaataa gaactaccca aattaggtag ttc
//
LOCUS       DASHHB010000126         3671 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.134, whole genome shotgun sequence.
ACCESSION   DASHHB010000126 DASHHB010000000
VERSION     DASHHB010000126.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3671)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3671)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3671
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.134"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..277
                     /locus_tag="U2K71_002800"
     CDS             <1..277
                     /locus_tag="U2K71_002800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000136908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific recombinase"
                     /protein_id="HEM7316805.1"
                     /translation="ELIKLANKGQDYEHLADEIDELRDKRQLLLVEDASLSGENERIN
                     ELIEFIRKNKFRTLEYDDKLVRKIIQSVTVYEEHFVISFKSGIEMEI"
     gene            420..641
                     /locus_tag="U2K71_002801"
     CDS             420..641
                     /locus_tag="U2K71_002801"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7316806.1"
                     /translation="MTTAEMIKELCEQMNISVSELARRIGQTPQNFNKKLQRETVTLD
                     ELKAIADVLGVKFVQAFILPDGNEIKIGN"
     gene            657..1499
                     /locus_tag="U2K71_002802"
     CDS             657..1499
                     /locus_tag="U2K71_002802"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316807.1"
                     /translation="MKIITINDVEYAVFAANEGTSKPQPHIIETKSGTIPEGKQLSLL
                     KEYLKQNDISPIKGATTYWCIDKVLKLDSSKEKTISETIHKQKYLSLTEENIEKQHKF
                     VGASSNYGKEGLIIHDVLNAFPLHNDLNTIAMKIAVIDVTNSTHLSQYKSRLSLYDLA
                     KVILEIPNFDDRLAKGDPQLINIIARNIGAVNMFSFASKYCTYHNVEVCGRDDYSIFD
                     GIVKNTLPHYIQGLTTNKIDTWRRSFDYEAFNECVGKLLDENNIHIPFRRRKLDHFLW
                     YANR"
     gene            1562..3364
                     /locus_tag="U2K71_002803"
     CDS             1562..3364
                     /locus_tag="U2K71_002803"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exoribonuclease"
                     /protein_id="HEM7316808.1"
                     /translation="MGLFDFLKKKQGKVSKHVDYQLASSLFRTGKVFAEKLEVDISNV
                     DEFRKTFIAFDVETTGLNSSSDRIVEIGAVLFKEGVPIQSFGTLVNPKVRIAASASAI
                     NNITNEMLNSAPNEQEVYPQLVEFLGDALDGKITMCAHNASFDFGFLSNTFSRLGYDA
                     KIKYVDTLSLSRKHIKGIVNHKQETVAEYFGLINDTAHRAESDADICGKIFWGVLDII
                     EESLEEQKKQIEKMTPDIKELEACAFIQDIIEKKGCDTSWIRYRKNSNNYVDVTCLYT
                     FLKFKFAKKGNYIIIRKDATNGIDLPIEPCTVSEGGTTNVRVYFRSPFDLTPLSNYIF
                     SAYSDCYNSMNEYMSVSNYTKKEAEQSLSMLKSISKSAMKELLFDAKNRGYDETEVNL
                     NIEKVISREDVIINGLHNRVSLNKIRNIGNWEKGFDAGYPYWERGETARKNGKVDEAI
                     ALFDKARYNGYDAPALYDSYAKAYRKIKDYDNEILILDEGIMRKTRHDVGTLEARRDK
                     AIKLLFAKQEAERIAKEKSDFLKANKKEDAISEPSKKRGRQIIQMTDDGTIIKEFDTI
                     ASAVKEVGVNSKSIRDAANGVQKHAGGYYWKYKD"
     gene            complement(3423..>3671)
                     /locus_tag="U2K71_002804"
     CDS             complement(3423..>3671)
                     /locus_tag="U2K71_002804"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS982 family transposase"
                     /protein_id="HEM7316809.1"
                     /translation="ISIPPRKNMDKSEKLDHSLLGKQRKTVGTVFSSLEKLGCQNFNS
                     RSVKGLESRFESILLAYSVLLSRAQRRFEGTLRYSLGY"
BASE COUNT         1309 a          519 c          748 g         1095 t
ORIGIN      
        1 agaactcatc aagctggcta ataagggtca agattatgaa catctagcag atgagattga
       61 tgagctgaga gacaagcgac agctcctttt agtagaagat gcctccctca gtggcgagaa
      121 cgagcgaatc aatgagctga ttgaatttat ccgcaagaac aaattccgta ccttagagta
      181 cgatgataag cttgtaagga agataatcca gagcgttaca gtctatgaag aacacttcgt
      241 catatccttc aaatctggca tcgaaatgga aatatgaaaa ccagaagtaa atagcccatg
      301 attctacagt agagtcgtgg gttttctttt gcttgctgat tataataata ttttaataac
      361 tattgttatt ataaaccaaa tgatttataa tattcttagc gtaaacttgg aggtgctgta
      421 tgactacggc agaaatgatt aaagaactgt gtgagcaaat gaatataagt gtttccgaac
      481 ttgctagacg tattggacag actccacaga atttcaataa gaaattacaa cgagaaacgg
      541 taaccttaga tgagttgaaa gcgattgctg atgtgctagg tgtcaagttt gtgcaggctt
      601 ttatattgcc tgatggcaat gaaataaaga taggtaatta ggaggtgtaa tttgtattga
      661 aaatcataac aataaatgat gttgagtatg ctgtatttgc tgctaatgaa ggtacttcca
      721 aaccacaacc tcatatcata gaaacgaaat ctggtactat tccagaagga aaacaactga
      781 gtttattaaa agaatatctt aagcagaatg atatttcccc aataaaagga gcaacaacat
      841 attggtgcat tgacaaggtt cttaagcttg attcaagtaa agaaaaaact ataagtgaaa
      901 caatccataa acaaaaatac ttatctttaa ctgaagaaaa tattgagaaa cagcataagt
      961 ttgtaggtgc aagctctaat tatgggaaag aaggtctaat aatacatgat gttttgaatg
     1021 catttcctct tcataatgat ttaaatacta ttgcgatgaa gattgcggta attgatgtaa
     1081 caaattcaac acatctttct caatataaga gcaggttgtc tctatatgat ttggctaaag
     1141 taattctgga gattccaaat tttgatgatc ggttggcaaa aggagatcct cagttgataa
     1201 atattatcgc aaggaacatt ggtgcagtta atatgttctc atttgcatcc aagtactgta
     1261 catatcataa tgtggaagtg tgtgggagag atgattactc catctttgat ggtatcgtta
     1321 aaaatactct tccacactat atccaaggac taactacgaa taagattgat acgtggcgaa
     1381 gaagttttga ttacgaggca tttaacgaat gtgttggaaa actacttgat gaaaataata
     1441 tacatattcc ttttagaaga agaaagttag atcacttctt gtggtatgcg aataggtaga
     1501 atataagaaa aaggcatcgt aaaagtgatt ctggataaaa ttaatggggg aggaaataat
     1561 agtgggatta tttgattttc taaagaaaaa acaaggaaag gttagtaaac acgttgatta
     1621 tcaactggct tctagtctat ttcgtactgg taaagttttt gctgaaaaat tggaagtgga
     1681 catatcaaat gtagatgagt ttcgtaaaac ttttatcgct tttgatgtgg aaacaactgg
     1741 cctaaattct tcttcagata gaattgtaga gattggtgcg gtgctattta aagaaggagt
     1801 tccaattcaa tcattcggaa ctcttgtcaa tccaaaagtt agaattgcag cctcagcttc
     1861 ggcaataaat aatataacta atgaaatgct aaactctgct ccaaatgagc aggaagttta
     1921 cccccagttg gtagaatttt taggagatgc actagatgga aaaatcacta tgtgtgcaca
     1981 caatgcaagt ttcgattttg gatttttaag taacacattt tctagacttg ggtatgatgc
     2041 taaaattaag tatgttgata ctctgagtct ctctagaaaa catataaaag gcattgtaaa
     2101 tcataagcaa gagactgtag cggaatattt cggattaata aatgacactg cccatagagc
     2161 ggaatcagat gcagatatat gcggaaagat tttttggggt gttttagata ttattgagga
     2221 gtcgttggaa gaacaaaaaa agcaaataga aaagatgact ccagacataa aagaacttga
     2281 agcttgtgct tttattcaag atattattga gaaaaaagga tgcgacacca gttggatcag
     2341 gtatcgaaaa aacagtaata attatgtcga tgtaacttgt ctatatacat tcttaaagtt
     2401 taagtttgct aagaagggaa attacatcat tattagaaaa gatgctacaa atggaattga
     2461 tttacctata gaaccttgta ctgtaagtga aggcggtaca acaaatgtgc gagtctattt
     2521 tcgtagcccc tttgacttaa cgcctctttc aaattatatt ttcagtgctt atagcgattg
     2581 ttataattct atgaatgaat atatgagtgt tagtaattac actaaaaaag aagcagagca
     2641 aagccttagt atgctgaaat ctatttcaaa atcagcgatg aaagagttat tattcgatgc
     2701 aaaaaatcgt ggatatgatg agactgaagt taacctaaat attgaaaaag tgatatctag
     2761 agaagatgta attattaacg ggttgcataa tcgagtttca ttaaacaaga tacgaaatat
     2821 agggaattgg gagaaaggat ttgatgcagg ttatccatat tgggaacgtg gagaaactgc
     2881 taggaaaaat ggcaaagtag atgaagctat tgcacttttt gataaagctc gatataacgg
     2941 ctatgatgct cctgcgctat atgattctta tgctaaagct tatcgaaaaa ttaaggatta
     3001 cgataatgaa attctcattc ttgatgaagg aattatgcgt aaaacacggc acgatgttgg
     3061 tactttagag gctcgtaggg ataaggcaat taagctatta tttgctaaac aagaggcaga
     3121 gcggatagct aaggaaaaat ctgattttct aaaagcgaac aaaaaagagg atgcgattag
     3181 tgagcctagc aagaagcgtg gaagacagat tattcaaatg actgatgatg gaacaataat
     3241 aaaagaattc gatactatag cttctgctgt aaaagaagtt ggcgttaact caaaaagtat
     3301 tagggatgct gcaaatggag ttcaaaaaca tgctggagga tactactgga aatacaaaga
     3361 ttagcagata atctaagata acctaggatc ttcttaatag atacccgtgg tgctagttaa
     3421 ttttaatatc ctaaagaata tctcaaagtt ccttcaaaac gtcgttgtgc tcgacttaat
     3481 agaacactgt aagccagtaa tatgctttca aatctactct ctagcccttt aacagaacgt
     3541 gagttgaaat tttgacaacc taatttttct aaggaagaaa aaacagtccc aactgttttt
     3601 ctttgttttc caagtaagct gtggtctagt ttttctgatt tatccatgtt tttacgtggc
     3661 ggaatggata t
//
LOCUS       DASHHB010000127         3499 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.136, whole genome shotgun sequence.
ACCESSION   DASHHB010000127 DASHHB010000000
VERSION     DASHHB010000127.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3499)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3499)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3499
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.136"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            898..1821
                     /locus_tag="U2K71_002805"
     CDS             898..1821
                     /locus_tag="U2K71_002805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEM7316810.1"
                     /translation="MANEIVKYENRLNHIPLRKFNSREMNLFFSIASRVREKGVDEII
                     FSFHDLKKLSGYTERGEQFIKDLSSTYDKLLSINAWNDDGNTLTKFVAFTKYSIIRDK
                     QLVIISVNPEFKGLFNQLKNWTRFNLEQFTNLDSTYSKTMFRLLKQYRTQGWTEFSKE
                     KFFDLLDIPKSYWNSPSNIDKFILRPIKEELTPLFKGLTIRKKYGKGRGKPVIGYRFT
                     WKAEINHADDFSKGKQEDLRIKLFNIDHNGELTREEKWRAKDRILNLPLGTHEADFKQ
                     QQQTEEKQAEKRELSNKLKQDLLGNLQNLFD"
     gene            1837..2376
                     /locus_tag="U2K71_002806"
     CDS             1837..2376
                     /locus_tag="U2K71_002806"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016616811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEM7316811.1"
                     /translation="MADKTIKELAEELGVSKTAINKKVSDKNRKLWFAKNGNKFVINE
                     TGQKAIKSMFLVDNANLNRKLVGEKSKTKNHKNTNQVSELKLLSFLEKQLNRKDIQLQ
                     EKDNQIKQMQKLLDQQQQLTLQANQQIENLQEQLHLTYTEETPTTESTAFSEKKADNT
                     QPLIEKKWWQIWKSNRTNL"
     gene            3057..3230
                     /locus_tag="U2K71_002807"
     CDS             3057..3230
                     /locus_tag="U2K71_002807"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316812.1"
                     /translation="MLKKTVLVLGSISLGILGFSPMLLSVIGKNNSNRELKLNSNYYN
                     SPNEELFVKETDK"
BASE COUNT         1327 a          535 c          602 g         1035 t
ORIGIN      
        1 agtaccttta aaggtacttt cacattacga acgatacgtc cgtaggacgt actgaaaggg
       61 tgggacttat gagcaaatta atgcaaaatt tggctagcaa aaaagctaaa aaatatatgc
      121 taataatcga gcgaaataaa cactttttca agtgagcgaa tccgccatga caaagctgaa
      181 gcagtttatt tcagctttgt catggcgtgt gccgactttt ttaaaagtct aggcacacga
      241 gcaaatatct ttatcaagct ctaatttgcg ttttaagacg tttgaaacgc ctaagtatat
      301 aaatatataa gaataggttt aagagtgctt aaatcgaatt ttagagggca tttagacaat
      361 tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ttttaagaac aagataaggg
      421 atttatacta ggactgtctt aaaatgtctt aaacgaacgc tcagagcttc tcaaagggtt
      481 ccttatgaac ggcttttagc aaaggagccg gaggcgactg cgcagtataa tgtgagagca
      541 gagcgatcac acctttttag cgaaccgtag ggagcaaggt ttggaattgg cagttttgat
      601 aaattcgcga acctgtttag agaaaaaagg agtgattttt taggcgggaa aattagcctc
      661 ccctcttttt atatctcttt ttatatctct ttttatatct cttttatgtc ggctgtgtcc
      721 ttactctccc aagcaattca gagttttttc aagtaggttt tctacgcttt tcaagtaggt
      781 tttctacgct tttcaagtag gttttctacg cttttcaagt aggttttccg acattattga
      841 gtatttttat aaagtatgtt acttttattt catactttat aaaagtagga gaagcttatg
      901 gcaaatgaaa ttgtaaaata tgaaaataga ttaaatcata ttcctctgag aaaatttaat
      961 agtcgtgaaa tgaacttatt tttttcaatt gcttcacgtg tacgtgaaaa aggtgtagat
     1021 gagattattt tttcatttca cgatttgaaa aaactaagcg gttatacaga acgtggggaa
     1081 caatttataa aagatcttag tagcacgtat gacaaattat taagtattaa tgcttggaat
     1141 gatgatggaa atactttaac taaatttgtt gctttcacaa aatatagcat tatacgagat
     1201 aaacaacttg ttataatttc agttaatcca gaatttaaag gactttttaa tcaattaaag
     1261 aactggacaa gatttaatct agagcaattt actaatttgg atagcacata ttcaaaaact
     1321 atgtttcgat tattaaaaca atatcgaact caaggctgga ctgaattttc caaagaaaaa
     1381 ttttttgatt tactagatat tcctaaaagt tattggaata gtccgtcaaa tattgacaaa
     1441 tttatcttaa ggccaattaa agaagaactc actcccttat ttaaaggatt aactattcgt
     1501 aaaaaatatg gtaaaggtcg aggtaaacct gttatcggtt atcgttttac ttggaaagct
     1561 gaaattaacc atgctgacga tttttccaaa gggaaacaag aagatttacg tataaaatta
     1621 tttaatattg atcacaatgg agaattaaca cgagaagaaa aatggcgcgc taaagatcga
     1681 atactaaatc ttcccttagg cactcatgaa gctgatttta aacaacaaca acaaactgaa
     1741 gaaaaacagg cagaaaaacg agaactttca aacaaactca aacaagattt actaggaaat
     1801 ttacaaaact tattcgatta agcttaagga gtatttatgg cagataaaac aatcaaagaa
     1861 cttgcggaag aattaggagt tagtaaaaca gcaattaaca aaaaagtttc tgacaaaaat
     1921 cgaaaacttt ggtttgcaaa aaacggaaac aagtttgtga ttaacgaaac cggtcaaaaa
     1981 gctataaaat caatgttttt agtcgataac gcaaacttaa atcgaaaact agttggcgaa
     2041 aaatcaaaaa ccaaaaacca taaaaacacc aaccaagttt ccgaattaaa actcctttct
     2101 ttcctagaaa aacagttaaa tcgaaaagat atacagctac aagaaaaaga taatcaaata
     2161 aaacaaatgc aaaaattact cgaccagcaa caacaactca ctctacaagc gaaccaacaa
     2221 attgagaacc tacaagaaca gctccattta acttatacag aagaaactcc aacaaccgaa
     2281 tcaactgcct tttctgaaaa aaaagcagac aatacacaac cacttattga aaaaaagtgg
     2341 tggcaaattt ggaaatctaa tagaaccaat ctctaaaatt ttctagaagt tagttcggat
     2401 agtagaggaa aaagttgtac tacctgatgg ggagatcact caaattttta tgagaaagaa
     2461 aaaatagttt acaactgtaa acaatcaatg tttatagttg taaactatca aactatatgg
     2521 tgacttattg cttaaaatag gttgaaatct cactcaattt ttggtggaaa aacaaaataa
     2581 attctaatcg attttgttga aaaagtcata aaaaaacaaa tgtgatttta acaaaatttc
     2641 aaaaaaatcg tttgaaattt cgtttatttt ttttgagaat tttgatcatc gtagggtcat
     2701 tgtagaatca ttatgagatc atcgtgggat catcgaataa agtatgggaa aggcttgcgg
     2761 tgcttggtat taagggcatt ctccgcctcc caggttcttg accattttct cttataagtg
     2821 ggatttttac cacgttgaga gaaaatggtc aagaacctgg gaggcggaga aaacatacca
     2881 agcaccacaa gcaaaaaaag ttagagtaac ttccaaaaaa gaaaaatttt ttagaacaac
     2941 ttttgaaaat ttaattttga tttatatccg ttcaatttaa acatttgata aactgaaggc
     3001 tagtatttaa aatgtgtata caagtggtag cgttaaaaaa agtggaggta taacctatgt
     3061 taaaaaaaac tgtgttggta ctgggttcta tttctctagg aatattaggt tttagcccca
     3121 tgctattatc cgtaattgga aaaaacaatt ccaacagaga acttaaattg aattcaaatt
     3181 attacaacag tcctaacgaa gaattgtttg taaaagaaac ggataaataa taaaaaagac
     3241 tgctcttccg agcagccagt taaaaaataa tgagtgttta ttcttgaaaa agcaacgcgc
     3301 ttataaagcg ctaccaaaaa tttatcatat tttatgaaaa ataacaatcc agtaaaaaat
     3361 gactggtaaa aattcacaaa gaaacggaaa gaagcaactg cagaaaatca agccttaaaa
     3421 agagcttttt acctagacca attaaaaatc acaaagtgag cccctgtatc ggttgataca
     3481 agggctactt ggcatactg
//
LOCUS       DASHHB010000128         3275 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.137, whole genome shotgun sequence.
ACCESSION   DASHHB010000128 DASHHB010000000
VERSION     DASHHB010000128.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3275)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3275)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3275
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.137"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            5..589
                     /locus_tag="U2K71_002808"
     CDS             5..589
                     /locus_tag="U2K71_002808"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005876718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM7316813.1"
                     /translation="MAFPQLDLKSEKGPKAVIKTNRGDITVQLFPELAPKTVQNFIEL
                     SKKGYYDGVIFHRVIPDFMIQGGDPTGTGMGGESIYGESFEDEFSRELFNLRGALSMA
                     NSGPNTNGSQFFIVNNTNVPANMLGQLEGAGFPSEIIEAYKGGGTPWLDFRHTVFGHV
                     LEGMDTVDEIANVQRGPQDRPVHDVVIETIEISE"
     gene            complement(666..1859)
                     /locus_tag="U2K71_002809"
     CDS             complement(666..1859)
                     /locus_tag="U2K71_002809"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629534.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HEM7316814.1"
                     /translation="MTGGLKMIEFDKQISRKDTESVKWDAIAETYQMDDLLPLWVADM
                     DFLSPGGVAKAFSEYIKHGIFGYATLSDKLYQAIIHWERQHHAVELTKENIVFTSGVL
                     TSLAAAVQTFTKPGDSILIHDPVYPPFSSIIETNQRHIVRSSLLEKNNHFIMDLSDME
                     KKIEENNVKAMILCNPHNPGGRVWSKEELKQLSELCLKHQVILFSDEIHQDLTLFDHT
                     FTSMLTIDPNLDDLLVAFTSATKTFNLAAIKNSMVFIKNPEMKADFEKHLVMNQQHEI
                     NTFGLIGTQAAYETGGEWLSQLIPYLEKNVQTVNQFFEQYLPKVRIMNPEGTYLMWLD
                     FTAYISDDQELENTLVRKGKVVLNPGITFGPSGHGHMRLNIACPEETLLEGLNRIKYA
                     LETID"
     gene            1923..2303
                     /locus_tag="U2K71_002810"
     CDS             1923..2303
                     /locus_tag="U2K71_002810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HEM7316815.1"
                     /translation="MTYKIGQIIEGKVTGIQPYGAFVSLDNETQGLIHVSEVQSGYTK
                     NIHSLLKVGQPVTVQIIDIDEYSKKISLSLRTLEKTNPAVPYRRKRYFTNKNRKIGFT
                     TIAEQLPIWIEEALSELERKENKK"
     gene            2377..2739
                     /locus_tag="U2K71_002811"
     CDS             2377..2739
                     /locus_tag="U2K71_002811"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014387120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316816.1"
                     /translation="MASKRVAGTIMLHLEDGTKKFLVHSIDDKLEFALAELSEGKTGL
                     ANILNFLKEIVHIDVSKISLMELTNTHIKEENVPLFVFETEQKNQREELGEGYIWEEP
                     GQMRSVLGTYEVEGVPFF"
BASE COUNT         1112 a          536 c          620 g         1007 t
ORIGIN      
        1 caatatggca ttcccacaac tagatttaaa aagtgaaaaa ggaccaaaag ctgttatcaa
       61 aacaaaccgt ggtgacatta ctgttcaatt attccctgaa ctagcaccga agacggttca
      121 aaatttcatt gaactttcca aaaaaggtta ttatgatggc gtgatttttc accgcgttat
      181 tcctgatttc atgatccaag gcggcgatcc aactggaaca ggaatgggtg gcgaaagtat
      241 ttatggcgaa agttttgaag atgaattcag tcgtgaactg ttcaaccttc gcggagcttt
      301 atctatggca aattcaggtc caaacacaaa cggaagccaa ttctttattg taaataacac
      361 aaatgtgcca gctaatatgc ttggacaact agaaggtgct ggattcccat cagaaatcat
      421 tgaagcgtat aaaggtggcg gaactccttg gctagatttc cgacatacag tctttggaca
      481 tgtactagaa gggatggaca cagtagatga aatcgcaaat gtacaacgtg gtccacaaga
      541 ccgacctgta catgatgtag tgattgaaac aattgaaatt tcagaatagt ttggtatggt
      601 acggagtaat tatgaaaaag gacgaaacct agtaaggtct cgtcctttta ttttgaataa
      661 atgatttaat cgattgtctc taaagcatac ttgattcgat taagtccttc cagtaaagtt
      721 tcttctggac aagcaatatt caagcgcata tgcccatgac cggaaggacc aaacgtgatg
      781 ccaggattta atacgacttt tcctttccga acaagagtgt tctccaattc ttggtcgtct
      841 gaaatatagg cggtaaaatc taaccacatt aagtaagttc cttcagggtt cattattctc
      901 actttcggaa gatactgttc aaaaaactga ttaactgttt gaacattttt ttctagataa
      961 ggaatcagtt ggcttaacca ttctccaccc gtctcataag ccgcttgggt tccaatcagt
     1021 ccaaaagtgt tgatttcgtg ctgttggttc atcactaaat gtttttcaaa atcagctttc
     1081 atctctggat ttttgataaa taccattgaa tttttgattg cagctagatt gaaagtcttg
     1141 gttgcggaag taaaagcaac tagtagatca tctaaattag gatcaatcgt tagcatggaa
     1201 gtaaatgtat gatcgaataa agttaaatct tgatggatct cgtcactaaa cagaatcact
     1261 tgatgtttta aacacaattc gcttaactgt ttcaattctt ctttgctcca aacacgccct
     1321 ccaggattgt gaggattgca taaaatcatt gcttttacat tgttttcttc gatttttttc
     1381 tccatgtcag acaagtccat gatgaaatga ttattttttt ctagcaaaga gcttcgaacg
     1441 atgtgacgtt gatttgtttc tataatagag gaaaatggag gataaactgg gtcatgaatc
     1501 aaaatagaat cgcctggttt tgtaaaagtt tgtactgctg cagctaaact ggttaacaca
     1561 ccacttgtaa aaacgatatt ttctttcgtc aactccacag catggtgttg gcgttcccaa
     1621 tgaatgatag cctgatatag tttatcagac agagtggcat aaccaaagat tccatgtttg
     1681 atgtattcac taaacgcttt agctacccca ccgggagaca agaagtccat atctgctacc
     1741 cacaaaggaa gtaagtcatc catttgatat gtctcagcaa ttgcgtccca ttttacactt
     1801 tctgtatctt ttcttgaaat ctgtttatca aattcgatca tcttaagacc tccagtcatt
     1861 ttcgtcgctt ctttttttga gctatttaca gtataatgat aaatgcgaag agagggaagt
     1921 agatgactta taaaattgga caaataattg aaggaaaagt gacagggata cagccttatg
     1981 gcgcattcgt ttctcttgat aacgagacgc aaggacttat tcatgtttca gaagtacaat
     2041 ccggttatac gaaaaatatc cattcattgt taaaagtcgg acagccagta acagttcaga
     2101 tcatcgacat tgatgaatat tccaaaaaaa tcagtttatc tttaagaacg ttagaaaaaa
     2161 caaatccagc tgttccatat cgcagaaaaa ggtatttcac gaataaaaat cgtaagatcg
     2221 gatttaccac tattgcggag cagttaccta tttggataga agaagcatta tctgaactag
     2281 aacggaaaga aaataagaaa taaaaagtgt attttttctg aaaatgtgct agaattttta
     2341 tgtaagccat agaacaagtt gatcgaggtg gaaattttgg caagcaaaag agttgctggt
     2401 acaattatgt tgcatttgga ggatggtact aaaaaatttt tggtccattc aattgatgac
     2461 aagttggaat ttgcgttagc ggaactttct gaaggaaaga ctggtttagc gaatatactt
     2521 aatttcttaa aagaaatagt tcatatcgat gttagcaaga ttagtttgat ggaattgaca
     2581 aatacacata ttaaagagga aaatgttccg ttgtttgtct ttgaaactga acaaaagaat
     2641 caaagagaag aattaggcga aggatatatt tgggaagaac caggtcaaat gcgctctgtt
     2701 cttggaactt acgaagtaga gggagtgcct ttcttctaaa atacagatta agcgaacaaa
     2761 gaagtttgaa tttttttcaa acttcttttt ttattgtttg gtccaaaaaa tacaatgaaa
     2821 tgttctgcta aagatagttt tttagtgcat tttattttca aaatatgttg aaaacggttg
     2881 tataacgaat aataataatt gatcgaaaaa tcatttcaca aagtaatgct taatttatag
     2941 ttgaccaagg aagaaataca tgataaattt aacagcgttg attctgaagt ggatgttata
     3001 tcacatttct aaaaaattta aaaaatagtt attgacatgt tactaacgac atgataagat
     3061 aactgagtcg ttaaaaaaga accacttgcg aagcttgatt ctatcgaaaa aaacttcaaa
     3121 aaagttcttg acaaataaca aacgataagt tatgataaat gagttgctga aagatttcga
     3181 aaaacgaaat cgaaaaaaac ttttaaaaaa gagttgacaa tcgcttgtct ctctgatatg
     3241 atataaaagt tgctacggca gccaaaaacg aatcg
//
LOCUS       DASHHB010000129         3259 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.138, whole genome shotgun sequence.
ACCESSION   DASHHB010000129 DASHHB010000000
VERSION     DASHHB010000129.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.138"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            14..175
                     /locus_tag="U2K71_002812"
     CDS             14..175
                     /locus_tag="U2K71_002812"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316817.1"
                     /translation="MKKRQELLEEVYTERFGTKEERETVRFYSVSEEKNLDTTFIADL
                     YKENGVELK"
     gene            172..1476
                     /locus_tag="U2K71_002813"
     CDS             172..1476
                     /locus_tag="U2K71_002813"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM7316818.1"
                     /translation="MISITHTGNLFLDTCLSIMYFFLVSYPILGGFVWFIGVWCYVFL
                     YKHKQKEWVDVPLSVEPFITIMVPAHNEEIVIEDTIEYLMTKLNYHNYEVLVTDDGST
                     DQTPEILARLMKKYANLRVVRIEKNKGKAHAFNIGLAFAKGKLILSNDADTVPEPDAL
                     IRYVNYFIRPGARHIAAVTANMDVQNRTKLIAKSQTVEFSSIVGIIKRTQSAVFGGLY
                     AYSGANTMYRKEALIDVGGFRQDRATEDISIAWDHQLNDWLSVFAPEIIFFMEVPVTL
                     KMLYRHRKRWAKGGTEVWLTNFKKVMLHPFKHIGRTIIFIDQTLSIVWSIFFCISVVL
                     FAGLIGHYVYQGNYEQIYITFTFSFVFICFEMVAGFFQLLASLIVDDRRRKLKYLLFA
                     PLYMLLFWIVNAITIVTTFIPAVKTILGYGSGTWKSPERTKK"
     gene            1572..2441
                     /locus_tag="U2K71_002814"
     CDS             1572..2441
                     /locus_tag="U2K71_002814"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015370988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM7316819.1"
                     /translation="MGKVMKKRMKPLIWPIAVSSIFLLGQTAFAETSEPAQTEPSTTI
                     SSSGAENGASETEMTSNVPTDNSGETISSSEDTSINSSTSSDQIDHLQDLENKRKELE
                     EALEAAKNKLKEDQAAIDQDLADLKNKVGNDRAQLEKKLAEAKAKAEAVQTDLDGKIQ
                     QAKEFNQQLNQVAQTASGKLSQTQADITAASQAIHGKISEAQTVANDFEAKKADLAAS
                     FQRIKDILHSAGEKSVTSGSTTETVQQTTEPSKSTSQTLPKTNEKTASVSYSLAGAGV
                     LVAAAYGIIRKKK"
     gene            complement(2553..2873)
                     /locus_tag="U2K71_002815"
     CDS             complement(2553..2873)
                     /locus_tag="U2K71_002815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HEM7316820.1"
                     /translation="MITTTTNSVEGKAVVAYKGIVFGEVITGIHAFKDLEAGLRNIFG
                     GRSKSYENELMGAREEALAEMAERAKALGADAIIGVKMDYEVLGSDNGMLMVTCSGTA
                     VSTR"
     gene            3043..>3259
                     /locus_tag="U2K71_002816"
     CDS             3043..>3259
                     /locus_tag="U2K71_002816"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7316821.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVL"
BASE COUNT         1088 a          620 c          646 g          905 t
ORIGIN      
        1 tgaagatgaa aaattgaaaa aaagacaaga attacttgaa gaagtgtata cggaacgttt
       61 tggaacaaaa gaagagagag aaacggtccg tttttattct gtctccgaag agaaaaatct
      121 agatacgaca ttcattgcag atttgtataa agaaaatggg gtggaactga aatgatctca
      181 atcacccata caggtaattt attcttagat acttgtttgt ctattatgta tttttttctc
      241 gtttcttatc cgattttagg cggatttgta tggtttattg gtgtctggtg ttatgtgttt
      301 ttatacaaac ataagcaaaa agaatgggta gatgttccct tgagtgtcga accgttcatc
      361 acgatcatgg tgccagccca taatgaagaa attgtgatcg aagatacaat cgagtatttg
      421 atgacgaaat tgaattatca caactatgag gtattagtga cagacgatgg ttcaacggac
      481 caaacaccag aaatattagc tagactgatg aaaaaatacg ctaatcttcg tgtggttcgg
      541 atcgaaaaaa ataaaggaaa agcccacgcg tttaatatcg gacttgcctt tgcaaaaggg
      601 aaattgattt taagcaatga tgcagacacg gtaccggagc cagatgcact gattaggtat
      661 gtgaattatt tcattcgccc tggtgcaaga catattgctg cggtcactgc aaatatggat
      721 gtccaaaatc gtaccaaatt gattgcaaaa tctcaaacgg ttgaattctc aagtatcgtg
      781 ggcatcatta aacgaacaca gtcagctgtt ttcggtggtc tttatgcgta cagtggagca
      841 aatacgatgt atcgaaaaga agccttgatc gatgtgggcg gattcagaca agatcgagcg
      901 acagaagaca tcagtattgc ttgggatcat cagttaaatg actggttatc tgtttttgct
      961 cctgagatta tctttttcat ggaagtacct gtgacgttga agatgctata tcgacacaga
     1021 aaacgttggg caaaaggtgg aacagaagtc tggctgacga atttcaaaaa agtgatgctc
     1081 catcctttta aacatattgg aagaacgatc atcttcattg atcaaacatt gagtattgtc
     1141 tggtcaatat tcttttgtat ttccgtagtt ctttttgctg gattaatcgg gcattatgta
     1201 tatcaaggaa actacgaaca gatttatatt acttttacat tcagttttgt gttcatctgc
     1261 tttgaaatgg tcgcaggatt tttccagttg ctagcttcgc tgattgtaga tgatcgacga
     1321 agaaaattga aataccttct tttcgcacca ctatatatgc ttttgttctg gatagtgaac
     1381 gcgataacga tcgtgacgac tttcattcca gcagtcaaaa cgattttagg ttatggaagc
     1441 gggacatgga aaagcccgga acgtacaaaa aaataaaaga tgcaccgttt tttagacaaa
     1501 cagcctgttg tgtttatggt tcttccaaac atttttgctt ataataagaa attagttaaa
     1561 aagtagtaag gatgggaaaa gtgatgaaaa aaagaatgaa gccgttaatc tggccaattg
     1621 ctgtttccag tattttctta ttgggacaga cagcttttgc ggaaacttct gaacctgctc
     1681 aaacagaacc ttcaacaacc ataagctctt ctggtgctga aaacggtgcc tcggaaacag
     1741 aaatgacttc caatgtaccg acagataatt caggagaaac gatttcatct tcagaagata
     1801 catcaataaa tagttctacc tcttctgacc aaatcgatca tttgcaagac ttggaaaata
     1861 aaagaaaaga actcgaagaa gcattagaag ctgcaaaaaa caaattgaaa gaagaccaag
     1921 cagcaatcga tcaagattta gctgatctta aaaataaagt gggaaatgac cgtgcacaac
     1981 ttgagaaaaa gcttgccgaa gcgaaagcaa aagcggaagc tgtccaaaca gatcttgacg
     2041 ggaagatcca acaagcaaaa gaattcaatc agcaattaaa tcaggttgct caaacagctt
     2101 ctggaaagct aagtcaaact caagcggata tcacagcggc cagtcaagca atccacggaa
     2161 agatcagcga agcacaaaca gttgctaatg attttgaagc aaaaaaagct gacttagcag
     2221 ctagtttcca acgaataaaa gatattttgc attcggcagg agaaaaatca gtgacctctg
     2281 gaagcacgac agaaacagtt cagcaaacga cagagccatc caaatcaaca agtcagactt
     2341 tgccaaaaac aaatgaaaaa acagcttctg tcagttattc cttagcaggg gcgggtgtgt
     2401 tagtcgcagc agcttacgga atcatccgca aaaagaaata aaaaagagag gatgggaagg
     2461 tagagtacct cgaagttaga ctgaaaaatc taattcttga gtgccactac atgtcccatc
     2521 tctctttagt tattaaaaca gatgtcccct acttatctcg tggagacagc tgttccacta
     2581 catgtgacca tcagcatgcc attgtcgctg cctaatactt catagtccat ttttacgcca
     2641 atgatcgcat cagctcctaa tgcttttgca cgttcggcca tttcggcaag tgcttcttct
     2701 cttgcaccca ttagttcgtt ttcatagctt tttgagcggc cgccaaagat atttcgcaac
     2761 cccgcctcca agtctttaaa tgcatggatc ccagtaatga cctcaccaaa aacgatccct
     2821 ttataagcta ctacggcttt tccctctaca ctgttcgttg ttgttgtaat cattcgatca
     2881 ctcctctctt ttaattaaag tataacaaac ttcaagaagc actacacata ttttcccaaa
     2941 attagaaatc gccgattgta aaattaagct agacaactaa aaaagtcatt tgtgctacac
     3001 tcaaaatagt tccgtcgaaa gaattataag gagtgagtac aaatgaccta tacccatctt
     3061 acaccaactg aactcgtaat gatagaagct tatttcaatc aatcacactc tgtttcaaaa
     3121 gtagcccatc tggtacaacg ttctcgacaa accatttata atgtctatcg ttttcttaaa
     3181 tctggtggaa gcgcaataag ctactatgaa caatataaga aaaacaaacg taactgcgga
     3241 agacgtccga ttgtgttac
//
LOCUS       DASHHB010000130         3202 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.139, whole genome shotgun sequence.
ACCESSION   DASHHB010000130 DASHHB010000000
VERSION     DASHHB010000130.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3202)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3202)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3202
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.139"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..588)
                     /locus_tag="U2K71_002817"
     CDS             complement(<1..588)
                     /locus_tag="U2K71_002817"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEM7316822.1"
                     /translation="MDEKTELTVKYQNELNLVPLKNFNAKQMDLFFALCARMKDRGLK
                     KVRFSFEELKELSDYKMTATKAFVGDLEKLYKDMLNLTYRTEDEDSIRYFVLFTGFEI
                     NKTKKFVEVSVNPDLEYIINGLTAEFSRFELSAFTSLRSTYAKTLFRLLMQYRSTGYY
                     VVRIEEFRSLMDIPEYYQMGNIDQKVLNPALRELKK"
     gene            1241..1336
                     /locus_tag="U2K71_002818"
     CDS             1241..1336
                     /locus_tag="U2K71_002818"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000493114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I toxin-antitoxin system Fst family toxin"
                     /protein_id="HEM7316823.1"
                     /translation="MCFVFTNFICSLLVGLLSALFSYWLNNRNKK"
     gene            complement(1638..1883)
                     /locus_tag="U2K71_002819"
     CDS             complement(1638..1883)
                     /locus_tag="U2K71_002819"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316824.1"
                     /translation="MRLEKEKMEKFPYEYFQNLEKLEQAIQAIKFDLTDEKLLEYKNM
                     TPERKRSLEKLMQLVNEDGGISDAAFDAWSEFHGEYN"
     gene            2228..2512
                     /locus_tag="U2K71_002820"
     CDS             2228..2512
                     /locus_tag="U2K71_002820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316825.1"
                     /translation="MDILVRNIDPKFVATIDKRCQELTNKTGEKWSRNKYLKVLIESD
                     FDRQLTTYKKDQFDLLLDKFIAIQTENTQILSEYVYTNNQIIEMLIGGMI"
     gene            2515..2931
                     /locus_tag="U2K71_002821"
     CDS             2515..2931
                     /locus_tag="U2K71_002821"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001232859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316826.1"
                     /translation="MRKTSVQKHVTLYPSHVDVLDSIIEKNPDINNYSEAIRFLCMSY
                     EPDTNTTTTSNQAKLNAMGKEISIMTEIIAEFADLYLKDTGVLIGTNSKVYQEVKEKI
                     EAKLNPSNRNRPKNHQKETSQTVTSRNFDPTNFLKR"
BASE COUNT         1065 a          597 c          499 g         1041 t
ORIGIN      
        1 tttttttagt tctcgtaatg caggatttaa tactttttga tcaatgttac ccatttgata
       61 gtattctggg atatccatta agctcctaaa ctcttcaata cgaactacat aataaccagt
      121 agaacggtac tgcataagca gtcggaatag agtttttgca tacgtagaac gcaacgacgt
      181 gaaagcagat aattcaaatc tactaaattc agctgttaaa ccattaataa tatattctag
      241 gtccggatta acactgactt caacaaactt ttttgtttta ttgatttcaa aaccagtaaa
      301 caaaacaaag tagcgaatag aatcctcatc ttcagtacgg taagtaagat taagcatatc
      361 tttatagagc ttttctaaat caccgacaaa ggcttttgta gcagtcatct tataatcact
      421 taactctttt aattcctcga aagaaaatct aacttttttc aaaccacgat ctttcatacg
      481 agcgcataat gcaaaaaata aatccatctg cttagcattg aaatttttca gcggtactaa
      541 attcaattca ttttgatatt tgactgttaa ttccgttttt tcatccattc ctttcttccc
      601 cccttacaaa taaataataa cctttaacaa ccaactcgta aataggttgg ttgttattat
      661 acaaaaggat attagtactc agataaaaag ttgttagtac tcagataaaa agttgttagt
      721 actcagataa aaagttgtta gtactcagat aaaaagttgt tagtaccctt ctgtatccgt
      781 tgggagagta aggctcaact gccccctaaa aagggttcta aaaaatcttt aaaaaataaa
      841 taaaataaac gcgcgcgcgc gagcaaaatt tcccgccaaa aaatcaccca ctttttaaaa
      901 taaacaggtt cgcgcatgat attaaactgc caatctagct ccctatggtc gctgtaagaa
      961 ggagtgatcg ctacgctctc acattttact tgcagcagtc gcctttaggc tccttcgcca
     1021 aaaataattc attagggact cctttgtggc gtcctgagag ctcgtctaag ccgttctaag
     1081 acaagattcg ataaaaacta tgtccttcgt tctaaagcgc cttacaggcg gtcagggcaa
     1141 gccctaacaa ccccaaggca ataaaaaact taggcttatt tttgcttttt tattgtataa
     1201 ttgattgtcg cctatccgac agtcttgcga ctggaaggag gtgtgtttcg tgttcactaa
     1261 ctttatttgc tcccttcttg ttgggctttt aagcgctttg ttcagttact ggttgaataa
     1321 ccgaaacaag aagtaaaact taattaaggc gactcagccc acaaaaaaag ccccagagaa
     1381 tccgacctct ctggggcttt cgtgtgtttc gtgttcactt atttgcaatc aaattataac
     1441 agaaataaat atttatttca aaaatatcct acaaaagtct aaaatccgta attatttctt
     1501 atatgcaaca tttacaaata aagtcgctct gagagctcgt atttttcgat ttaagacgtt
     1561 ttatttagtt tttggtgtat ttcattggtc caagctaaaa aactcttaga agcgatttta
     1621 gagccattta aacccactta attatattct ccatgaaatt cgctccatgc gtcaaaagct
     1681 gcgtctgaaa ttccgccgtc ttcgttcact agttgcatta atttttccaa cgatcgtttt
     1741 ctttcgggtg tcatattttt atattccaac aatttttcgt ctgttaaatc gaacttgatt
     1801 gcttgaattg cttgctctaa tttttctaag ttttgaaaat attcgtaagg aaatttttcc
     1861 attttttctt tctccagtct cacaattttt ctagaaataa aatcacttct ttgccgtcta
     1921 tactctcaaa aaaaatgtcg tttgaaaaac taaataaatt tttgatcgtg catttttaaa
     1981 tttggttatc gattacacgt gcagcactcc cccccgaatt ttaaagagca taaggaaacg
     2041 tgtacccact ttctaaaaaa agtgggtaga taggctgtgc ctatcacgtt tctcagaaaa
     2101 aaatttgata tcactttttc gccatcgtgg gtagcttatg tatagcattt tggcaaaaaa
     2161 cagctataca aatatagtat ttgtatggta tacataaatt atacataatt gcaaaaggag
     2221 attttttatg gacattttag ttcggaatat tgatccaaag tttgttgcaa caatagacaa
     2281 acgttgccaa gaactcacga ataaaacagg agaaaaatgg agtcgaaata aatatctaaa
     2341 agttttaatc gaaagtgatt ttgaccgtca acttacgact tacaaaaaag accaattcga
     2401 tttattgctc gataaattta ttgctatcca aacagaaaat actcaaatat taagcgagta
     2461 tgtctacaca aataatcaaa tcattgaaat gcttatcgga ggaatgatat aaccatgaga
     2521 aaaacttctg tccaaaaaca tgttactttg taccctagtc atgttgacgt tttagattcc
     2581 atcatcgaaa aaaatccaga tatcaataac tactcggaag ccattcgctt tttatgtatg
     2641 agttatgaac ccgataccaa cacgaccact accagcaatc aggcaaaact aaatgctatg
     2701 ggaaaagaaa tttccatcat gactgaaatc atcgcagaat ttgctgattt atatctaaaa
     2761 gatacaggcg ttcttattgg cacaaattct aaagtatatc aagaagtgaa agaaaaaatc
     2821 gaagctaaac taaatccatc aaatcgaaat cgaccaaaga atcatcaaaa agaaaccagt
     2881 caaacagtca cttctagaaa tttcgaccca acaaattttc taaaacgata aaaaacagat
     2941 aggagttatt cctatctgtt ttttatttag aagccccata caatatgcta gaatgtacta
     3001 gcaatcttag tcggttgtaa gggacttgtc tacttgctta atgtaagggg ggcgagttct
     3061 tttttattta ttcttctaac cagttacgca gagaaatctt tggcaaatct gacgttaggc
     3121 ttgaaaaagt aaattctaat ttggcgattt tattgccctt ttttgctttt atttttgtga
     3181 ctttaagatc attgaagtat tt
//
LOCUS       DASHHB010000131         3166 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.140, whole genome shotgun sequence.
ACCESSION   DASHHB010000131 DASHHB010000000
VERSION     DASHHB010000131.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3166)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3166)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3166
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.140"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..433)
                     /locus_tag="U2K71_002822"
     CDS             complement(<1..433)
                     /locus_tag="U2K71_002822"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316827.1"
                     /translation="MNILNEKRYVWFAGKKYITTFKRLSELMQQTVKSAMPEGYKFNF
                     VQSSVSTESIYFSILSESRLFHFRISSHVKKTDVDIYTFRTNNYKNLNDLSRSISEYL
                     KGNSYLKIRFSHILILLPYLNENFTLIQVDKSFIASGNQGNM"
     gene            complement(992..1309)
                     /locus_tag="U2K71_002823"
     CDS             complement(992..1309)
                     /locus_tag="U2K71_002823"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318298.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM7316828.1"
                     /translation="MKEYTLVYSKSFQTSLETIIEEWENELALSPASIKRFVGAIQKA
                     LELTKNFPTMHEEVSEIYGMDIPTYRIVIGKSYALFYRIDHEKKEILVGRIFQSKQMK
                     LGF"
     gene            complement(1310..1564)
                     /locus_tag="U2K71_002824"
     CDS             complement(1310..1564)
                     /locus_tag="U2K71_002824"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM7316829.1"
                     /translation="MGKELKVRKIGNSVGVILPSSLGLKSGDTIQAKQEGNLFILDTT
                     QIAKEHDRKLIEESFQDFEKGLTVSEIEMVKAFGKYGWSE"
     gene            complement(1824..2042)
                     /locus_tag="U2K71_002825"
                     /pseudo
     CDS             complement(1824..2042)
                     /locus_tag="U2K71_002825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002391850.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
     gene            complement(2602..2688)
                     /locus_tag="U2K71_002826"
                     /pseudo
     CDS             complement(2602..2688)
                     /locus_tag="U2K71_002826"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339775.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
BASE COUNT         1060 a          549 c          437 g         1120 t
ORIGIN      
        1 tcatatttcc ttgattacct gaggcaatga aagacttatc tacttgaatt agggtgaaat
       61 tctcattcaa atatggtagt aaaattaata tatgtgaaaa tcgaatcttg agatagctat
      121 tccccttgag atattcactg atacttctac tcaaatcatt taaattttta taattatttg
      181 tacggaacgt atatatatca acatcagttt tctttacatg agaacttatt ctaaaatgaa
      241 aaagcctact ctcagaaaga attgaaaaat agatactttc agtagatact gaagattgaa
      301 caaaattaaa cttgtatcct tcaggcatag cacttttaac agtctgctgc attaactcgg
      361 ataatctttt gaaagtagta atatattttt taccagcaaa ccatacatat cttttttcat
      421 taagtatgtt cactatctct cctccattaa ttatatttag catcaaatag ttattgtacc
      481 tatattttat cccattcctt ttaaaacaag aatacgtaca aagtaaaatg ttgtaaatag
      541 tctagcaggg atgtatattt tcatgtacca acataatatt taagaagttt cccatagata
      601 tattggactt cctcttttaa aacacaaaaa taaatactac cttatttagg tagtatttat
      661 taaaagttac gtcaattttg attaaaaata tactacttga acctctcatg agtaaagtca
      721 cgaaattttt gggaatagag cgtacttgat atcttatttt ttcactaata gcagtgtctc
      781 ttattcacca agcgatcccc gtagttccta cggttcttaa tttttctcta gactaatgat
      841 tgtatcttta gaccttcggc cataacatgg agcaaaataa aaaggtacta agtcttatat
      901 atcaatcttc gagtaaccga cggttaacaa ccatatcgag cggcattaat cacccacctg
      961 tagaggatga gcgacttttg cctaattatt gttaaaagcc tagtttcatt tgtttagatt
     1021 ggaaaatacg tcctacaagt atctcttttt tttcatggtc gatccgataa aataaggcat
     1081 agcttttgcc gatcacgatc cgatatgttg ggatatccat tccatagatt tcggaaactt
     1141 cctcatgcat agttggaaaa tttttggtta gttctaaagc cttctggatc gcacctacaa
     1201 atctttttat gctggctgga gaaagagcaa gctcattttc ccattcttct attatcgttt
     1261 caaggctggt ctggaaactt ttagaataaa ctagcgtgta ctctttcatc tattcactcc
     1321 agccgtattt tccgaaagct tttaccattt caatttctga aactgttaaa cctttctcaa
     1381 agtcttggaa actttcttca atcaatttgc gatcgtgttc ttttgctatt tgagtagtat
     1441 ctaatatgaa taagttgcct tcttgtttgg cttggatagt gtctccgctt tttaatccaa
     1501 gagagcttgg aaggatcact cccacgctat tgccgatctt tctaaccttt agttcttttc
     1561 ccatgttatc acacttcttt tagtttttga taaataaatt ataacatgtt ataacgacaa
     1621 ttgccaacgg tgtttcttaa gggattccac tccatattat tattggacaa aataagccac
     1681 ttagaacgct gaaaacatat acaaacgata tcaaaatacg agtgaactac tcaccataca
     1741 cttccgttaa catccattta agtaggtatt tttattatat atagatttaa catatgtcgg
     1801 tcggttgatt gatatagcta aagttataac tcaaccgaag ccgattcatc tacacgaata
     1861 aagttgcgag tgttctcggc taattcataa aaattgttat gtgttccata tagcaatact
     1921 ggaataactt tattttcttg aatctcataa gcaactctat acacggtgac cgcatactta
     1981 aatccattag accaattacc tgataaacct ccagtttttt gctcttactt aaatggatca
     2041 atagcaattt catcacaaac ctatagagga tgagtgactt ctgcctaatt acgttaaaaa
     2101 atattgaaag taactctatg tagcaaaaaa tatctagaat ttcatttcct caaaactgat
     2161 cgatattgta tctaaaaata tatactacct aaaataggta gtatatatta aaatatgtgc
     2221 ttttgttaga aaaatcaata cataaattat catatctgta tatgtcattt aggtatttca
     2281 gttatgttat aatagttttg ctgtctgcta ccccggccta atctggcaag gggaggagga
     2341 gatataatga aatacctttt tgattttgtt tttgcaatca agccatttgc agaggtaact
     2401 gcacactaca tctgcaagtg gcttgattgc aaaattgcca aaaaagacag taagcctacc
     2461 agttaattct ataatataga aacgtaaaaa aaaccccatc agcctacacg ctgatggggt
     2521 ttgatgatat aatgaaatac ctctttgtta ctcataatta taccataatt agtcacgaat
     2581 gcaaccaata gaaaaaacta cttagaacgg tagatcatcg ctaataacaa tgcattgtcc
     2641 accaaatgga ttttgtgaag cattaaatac attgttatta attgtcgctg tttttaacta
     2701 ggcctctccc cttctattat aaaagaagat atatattatt tcttaattac ctactacctt
     2761 aaaccaaaat ttaagtacta cctaatttaa gtagtactta aataatctaa aaaatagtta
     2821 tcccaattgt tatgctttta taatacgatt atttgtaaca aaatgaatat tatataatta
     2881 aacactacca tatttaggta gtgtttaatt attagcaaaa caaaaaatat ttattctatt
     2941 ctcttaactt caactaagtt atgctgcaca cctttatata aatcatatgc aactcattag
     3001 ctagacaaac tacaagcatg tagttttatc tgttaattat gtactaccta aataaagtag
     3061 tttataatta acaggtatat atatttaaaa atttaagatt aattttttta tgacttgtac
     3121 aaatttatct gatattctct tattaagaac tacctaattt gggtag
//
LOCUS       DASHHB010000132         2975 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.142, whole genome shotgun sequence.
ACCESSION   DASHHB010000132 DASHHB010000000
VERSION     DASHHB010000132.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2975)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2975)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2975
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.142"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            50..1438
                     /locus_tag="U2K71_002827"
     CDS             50..1438
                     /locus_tag="U2K71_002827"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-directed DNA polymerase"
                     /protein_id="HEM7316830.1"
                     /translation="MYKERDDLNYTLIINKDGNYSWRPLVLLHPILYVDLVNCLTKED
                     HWEELKKRFSEFKKDKNINCYSIPVEASKGSKRTDLGETILNWWEYFEQKSLAQNIKY
                     QYCMFTDIADCYPSIYTHSITWAMYGKEKVKNDENKCSGSLGDKIDKKISGMQHNQTN
                     GIPQGSVLMDFVAEIVLGYVDLRLSKELKKLENLKDYKVLRYRDDYRIFSNNRNDLEQ
                     IMKVLSEVLYDLNFKLNSSKTKLSDDIIIDSIKKDKLYWEEIKASIRMNATNLDGDRE
                     IRYSINLQKHLLEIKKLADKHPNSGQLKKALSEFNKERIIKITSKPNDLLPLIGILVD
                     IMINNPGSLPQCVLIIGQLLNFENESKAREIVEMIINKYALKPNTEFMMIWFQRLTTI
                     FGEMFDSVLSSEFVSKIKDPVSNQMFSVDWLNEDAKKSFYEGTIKDEEKYIKMSKKIY
                     ITEFDDFNDYPF"
     gene            1776..>2975
                     /locus_tag="U2K71_002828"
     CDS             1776..>2975
                     /locus_tag="U2K71_002828"
                     /EC_number="2.1.1.72"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction-modification system subunit
                     M"
                     /protein_id="HEM7316831.1"
                     /translation="MTEQIEKNSKSLYQALWNSADILRSKMDANEYKSYLLGLVFYKY
                     LSDNMLRYVSVLLEEETDDLNKAQDLYVEAFKDADIKDDLLYELKDEFSYTIAPALTF
                     TAQVAAIHDGSFQLEDLVQGFRDIEQSSENFENLFEDIDLYSKKLGSTPQKQNKTIAD
                     VMKELEGLNMAGHAGDVLGDAYEYLIGQFASESGKKAGEFYTPQPVAKLMTQIVLQGK
                     EDKKGFSVYDATMGSGSLLLNAKKYSHQPGTISYFGQELNTSTYNLARMMARMNMILH
                     GVPIANQHLHNADTLDQDWPTEEPTNFDGVLMNPPYSAKWSADKGFLDDPRFSAYGVL
                     APKSKADFAFLLHGYYHLKDTGVMAIVLPHGVLFRGGAEGKIRKALLENGAIDTVIGL
                     PANIFFNT"
BASE COUNT         1090 a          462 c          541 g          882 t
ORIGIN      
        1 ttcaaaaaaa aaccaaccga aagatataaa agatattgta gatacacgaa tgtataaaga
       61 acgtgatgac ttaaactata cgcttattat caataaagat ggaaactatt catggcgtcc
      121 tctcgttcta ctacatccta ttctatatgt agatttggtt aattgtctca ctaaagaaga
      181 ccattgggag gagttaaaaa agaggtttag tgaatttaaa aaagataaaa atattaactg
      241 ttattctata cctgttgaag cttccaaagg ttcaaaaagg acagatcttg gtgaaacaat
      301 actaaactgg tgggaatatt ttgaacaaaa aagcttagct cagaatatta aataccaata
      361 ttgcatgttt actgacatcg ctgattgtta tccatcaatt tatactcact ctataacatg
      421 ggccatgtat ggtaaagaaa aagttaaaaa tgatgaaaat aagtgttcgg gctcacttgg
      481 cgataaaata gataaaaaaa tatctggaat gcaacacaat caaacaaatg gtatccctca
      541 gggttccgtt cttatggatt ttgtagcaga gatcgttcta gggtatgttg atttgagatt
      601 atcgaaagaa ttaaaaaaat tagaaaattt aaaagattac aaagtactca gatatagaga
      661 tgattataga atattttcaa ataatcgtaa tgatttggaa caaattatga aagtgttatc
      721 agaagtctta tatgatttaa attttaagtt aaattctagt aaaacaaaat tatcagatga
      781 tattatcata gattctatca aaaaagataa gctctattgg gaagaaatta aggcaagtat
      841 aagaatgaat gccacaaact tagatggtga tagagaaatt cgttatagta taaatttaca
      901 aaaacattta ttggaaataa aaaagttagc agataagcat cctaattcag gccaattgaa
      961 aaaagcactt tctgagttta ataaagaacg cattattaaa atcacttcaa agccgaacga
     1021 tcttttacca cttattggta tcctggtaga tatcatgata aataacccgg gttctttgcc
     1081 gcagtgtgtc ttaattatag ggcagttatt aaatttcgaa aatgagagta aggctagaga
     1141 aatagttgaa atgattataa acaaatatgc tttaaagcca aatactgaat ttatgatgat
     1201 atggtttcaa agattgacca ccatttttgg agaaatgttt gatagtgttt tatcatccga
     1261 atttgtatct aaaatcaaag atcctgtctc taatcagatg ttttctgtag actggctaaa
     1321 tgaagatgct aagaaatctt tttatgaagg gaccattaaa gacgaagaga aatatattaa
     1381 aatgagtaag aaaatatata ttactgaatt tgatgatttt aatgattatc ctttttaagt
     1441 gaaaatttag aatgaatata aaaaacatta caatctaatt tttttaaaaa aatgttatat
     1501 cggaagtaat ctcttgattt ctatactata agataagaaa accactattt aacaaggtta
     1561 ttcgaacctt attttaagtt acttattatt taatgtatcg aaaaaggtga aataggtttg
     1621 ttataataag aattaagtaa gacatgaatt gtctaagcaa atgtagctta tccatttcat
     1681 taaaatacat atggtgaaaa aacaagttca aatagtaact aattttttaa aaaactgatt
     1741 tataaaaaat acaacacgaa tgaggaaaat aaccaatgac agaacaaatc gaaaaaaatt
     1801 caaaaagctt atatcaggca ctgtggaatt ccgcagatat cctgcgttca aaaatggacg
     1861 ctaatgaata caagagctat ttactaggct tggtctttta taaatactta tccgacaaca
     1921 tgctgcggta tgtctccgtt ctattggagg aagaaacgga cgacctaaat aaagcacaag
     1981 acctttacgt tgaagcattt aaagatgcag atataaagga tgatctcttg tatgaactaa
     2041 aagatgagtt ttcatatacc atcgcacccg cattgacctt taccgcgcaa gtggcagcca
     2101 ttcatgatgg atcttttcaa ttagaagacc tggtacaagg tttccgtgac attgaacaat
     2161 ccagcgaaaa ctttgagaac ttatttgaag acattgatct gtattctaaa aaactggggt
     2221 caaccccaca aaaacaaaat aaaaccatcg cagatgttat gaaagaatta gaagggttaa
     2281 acatggcggg gcatgctggc gatgtcttag gcgatgccta tgagtactta attggccaat
     2341 tcgcatctga atccggtaaa aaagccggtg aattctatac cccacaaccg gtcgcaaaat
     2401 tgatgacaca aattgtttta caaggaaaag aagataaaaa aggcttttct gtttacgacg
     2461 cgaccatggg ttcgggctcg ttacttttga acgctaaaaa atattcccat caaccgggaa
     2521 ccatttctta tttcggacaa gagttgaata cgtctaccta taatttggcg cgtatgatgg
     2581 cgcgtatgaa catgattttg catggggttc ccattgccaa ccaacacttg cataatgcgg
     2641 ataccttgga tcaagactgg ccaacagaag agccaacgaa cttcgatggg gtgctcatga
     2701 acccaccgta ttctgcaaaa tggtcggctg ataaaggctt cttagatgac ccacgcttct
     2761 ctgcctatgg cgtattagcc cctaaatcaa aagcggactt tgctttctta ttacatggct
     2821 actatcattt gaaagatacg ggcgtcatgg ccattgtttt gccacatggg gtgctcttta
     2881 gagggggcgc agaagggaag atacgcaaag ccttgttaga aaatggtgcc attgacaccg
     2941 tcattggctt acctgctaac atcttcttta atacg
//
LOCUS       DASHHB010000133         2959 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.143, whole genome shotgun sequence.
ACCESSION   DASHHB010000133 DASHHB010000000
VERSION     DASHHB010000133.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.143"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..280)
                     /locus_tag="U2K71_002829"
     CDS             complement(<1..280)
                     /locus_tag="U2K71_002829"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YfbU family protein"
                     /protein_id="HEM7316832.1"
                     /translation="MDSFQRLSLVNQFELLAKFEDQNRSYYERKIEILREGYEYHYDD
                     EIWSDLSEPFPKEDSRFVLDVLNMYRDINFSKSKLNLDENQITNSYYTH"
     gene            742..2124
                     /locus_tag="U2K71_002830"
                     /pseudo
     CDS             742..2124
                     /locus_tag="U2K71_002830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010726506.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
     gene            complement(2360..>2959)
                     /locus_tag="U2K71_002831"
     CDS             complement(2360..>2959)
                     /locus_tag="U2K71_002831"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016631531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HEM7316833.1"
                     /translation="TMKKEFPIPISWTTDVNAAAYGEYVFGSGKGLSSVVYYTIGTGV
                     GGGALQEGRFIEGFSHPEMGHMLVVPHPKDDFAGSCPFHGNCLEGMAAGPAIEKRLGK
                     KGQEVAEDDPYWEIEASYIAQCAYNTTLMFSPDVIIFGGGVMKQRHMLQNVHDAFAKL
                     VNGYVETPELSKYIVTPALEDNAGTLGCLALAREAVLHS"
BASE COUNT         1015 a          562 c          518 g          864 t
ORIGIN      
        1 aatgagtata gtatgaatta gtaatttggt tttcatctaa gtttagcttt gatttactaa
       61 aattgatatc tcgatacatg ttcaatacat ctaatacaaa tcgagaatct tctttcggaa
      121 atggctcaga aagatcactc caaatttcat catcataatg atactcgtaa ccttctctca
      181 atatttcgat ttttctttca taataagatc tgttttggtc ctcaaattta gccaataact
      241 caaattgatt tactagtgac aatctctgaa aactgtccat taaatgtttc ttcctatctt
      301 gttcattaac aaaaaattat aagcccttac aacgaatatt atatattaaa tttaattgta
      361 ggtgaacacg aattttataa atatttatgg tgttaatgac aaatgatatc cgactcctct
      421 tcatacgttc tggactattt gctaatagat taaaatttca gcatctattt ctgaccataa
      481 ataaatgagc tgccgaaaag ctgccaaatc agacttgaaa ataaaggatc aaaaactttt
      541 aagtttctga ccccgtaact cacacttcaa aatcttactt aaaaaggagt ctgaaagaaa
      601 aaaagagtat tcaaaatttt tttctaacta gctacatcac atctatgcat tgacactcaa
      661 agaaccagaa taagtcatca gaaagcaagg gggtgcggac ataatcctgg acgaaaaaat
      721 aaacttagga ggaatgtccg tgtgttttcc cacaaagagc gtgtaagggc aattcagtta
      781 tttttaaaat acgattgctc ttatgctgca accattcgag aattagggta tccgtcgatt
      841 ggtgcactac ggaaatggta taaagagtat ttggaatccg gagagctcca tcaggagcac
      901 cgaaaaaaat caaagtattc tgaagaacaa aaacgaatag cggtgaatca ttattttgaa
      961 tatggtcaat gttatgcacg aaccattcgc atgcttggtt atcctaatag agagtccttg
     1021 cggcagcggt gtgaagaact tgcaccagga gctagaaaac tccgaaaaag tgcggtaaag
     1081 ttaacacaag atcaaaaaga agctgtgttg aaaaaattct ataaaccgca aactaatagg
     1141 aaaaatttag ccgaatctgg aggaattagt agagttactc tatatcaatg gaaaaacacg
     1201 gttcttggaa aggattttcc attgtgtatg actttaaaaa atcaggaaaa acaaaaagag
     1261 ctacttttaa aggaagtaga agacttacaa aaacaagtac accaattaca gctcgaaaaa
     1321 gcattactcg aaggagcggc tgaactgcta aaaaaagaaa aaggcgtcaa tcttctttgt
     1381 ctatcaaatc aagaaaaaac gatattgatt gatgccttac gaaatcagtt caccttgaaa
     1441 gagcttcttc aacagctcca gtttcctaaa agcagctatt tttatcaaaa acaagcactt
     1501 gagaaaccag ataagtatta caaagaacgt caaattaatt atcacgattt tcaatcataa
     1561 cttctgtgcg tatggttacc gtagaattca ccaagcttta aaaaatatgg gtaaaaagct
     1621 ttctgaaaaa gttgtacgta gattgatgac tgaggaaaat ctatttgtta aattttctag
     1681 acgaaaaaag tattcttcgt atgctgggga aatttctcct gcacacccca atttattaaa
     1741 tcggaatttt aaagcaaaga agccaaatga aaaatggtta acagatatta ctgaatttag
     1801 aattctcgca gggaaaatct acttatcacc acttgtcgat tgttacgatg gtgctattgt
     1861 tagttggact attggaacaa agccaacttg cttcactcta tgtctaaaaa gagttattca
     1921 ccagataatt cagcgtgcga aggtttcttt ggacgattaa aaaacgagat gttttatcaa
     1981 cgtgactgga agaatacaac aatcaatcag tttataaaca aattggatga ttatattcat
     2041 tggtataata atcaacgaat taagttatct ttaggaaggc taagccctct tcaatatcgg
     2101 aagaagcaaa gatatatttg ttaaaaaggt ccaaaaaaaa tatccgcacc cccttttttt
     2161 tgtaaaaaac tattgcacaa tacaagtagc ctacggctat cgaaatttcc ataactttcg
     2221 agcaagaact tatctcattc aaggcttgat cttccaagta aaacaaaaac cagttaagca
     2281 ctctgcctaa ctggttatga ctaaattttc agttgtatct ttctctacca acactaaatg
     2341 tcgaagagcc ttttttcgtt tatgaatgta agaccgcttc tcgagccaat gccaaacaac
     2401 cgagcgttcc agcgttgtct tctaatgctg gggtcacgat atatttgctt aactcaggtg
     2461 tttcgacata cccattgacc aattttgcaa atgcgtcgtg gacgttttgc agcatatggc
     2521 gttgtttcat cacaccaccg ccaaaaatga tcacatctgg agaaaacatc aaggttgtat
     2581 tgtaggcaca ttgagcaata taggaggctt cgatttccca ataaggatca tcttctgcaa
     2641 cctcttgtcc ttttttccct aagcgttttt cgatggcggg tccagcggcc atcccttcta
     2701 aacagttgcc atgaaatgga cagctgccag caaagtcatc ttttggatga gggacgacga
     2761 gcatatgccc catttctgga tgactaaacc cttcgataaa gcgcccttct tgtaaggcac
     2821 cgccgccaac acccgtaccg atcgtatagt aaacgacact gctcaatcct ttgccactgc
     2881 cgaagacata ctcaccatag gcagccgcat tgacatctgt tgtccaagaa atcgggatcg
     2941 gaaattcttt tttcatggt
//
LOCUS       DASHHB010000134         2944 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.144, whole genome shotgun sequence.
ACCESSION   DASHHB010000134 DASHHB010000000
VERSION     DASHHB010000134.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2944)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2944)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2944
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.144"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            93..422
                     /locus_tag="U2K71_002832"
     CDS             93..422
                     /locus_tag="U2K71_002832"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM7316834.1"
                     /translation="MITKTRKQGNSIMLTVPKDFNVPNGVEVEAKLVENGILYEFVEP
                     QKEFYDFSEDILSDIIAEGYDKDEILVEFKNRKNKMHSSFRDIAEDTLTNSKVMTKEE
                     LATEIGL"
     gene            412..699
                     /locus_tag="U2K71_002833"
     CDS             412..699
                     /locus_tag="U2K71_002833"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002292679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM7316835.1"
                     /translation="MAYEIVPSKHVIKYLKKLKEKPLKEKFLNLIYDEIAIDPYKGEQ
                     KTGDLSGIWSSGFKYAGTTYRVAYEIIENKVIPVLLCGTHENFYEQLKKLI"
     gene            983..1840
                     /locus_tag="U2K71_002834"
     CDS             983..1840
                     /locus_tag="U2K71_002834"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HEM7316836.1"
                     /translation="MVAKKYVIGNFKGGVGKSTCAQMLGFESAVNEGKRTLIIDLDMQ
                     GNTSDVMNLTHMNFTNEEGGGEGEPIVYENTVTDVLINNLPPKEAVYKVINNLYILPA
                     DMSFEMYDDWIKDQYNDSLSQFKYMKEKLEPLFDEFDVIYLDVPPSISVYSKSAMYIA
                     DWAIVVLQTQVKSMRNAMQYLEYMDFFVKEFDTNLYVAGVIPFMLESGDAVDQEMYKQ
                     AQEIYGEHLIKNVVLKNARLKRYDGSGITMEKTKKGLLKQWDRKSHELFISILKELEE
                     HENWYAEVE"
     gene            1841..2389
                     /locus_tag="U2K71_002835"
     CDS             1841..2389
                     /locus_tag="U2K71_002835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316837.1"
                     /translation="MADIKGLDIFGNNKGLAKIKKEAEAKKKQQAITPSENKQLEVHQ
                     VQEIKTSSNVERQSRGRTKKVGAPIRKFDKVYSTKHPLKLSTLLNATSRVLVEKYETN
                     MTRDELLRKALDEYIKQNLTQEDKQDLFNDVVKELDLFREKYPIVPELDDRGEIIRTT
                     SQIKQDTEDSLRKGWGMNNKVR"
     gene            2649..2944
                     /locus_tag="U2K71_002836"
                     /pseudo
     CDS             2649..2944
                     /locus_tag="U2K71_002836"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002348804.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT         1114 a          377 c          592 g          861 t
ORIGIN      
        1 ttacgagtca cagtaattac ttgtaaatac attatctcgt ggtataatgt atttactaag
       61 taattacata ctgttagaaa tgagggattt aaatgattac aaaaacaaga aaacaaggca
      121 attcaatcat gttgacagta cctaaagatt ttaatgttcc taatggagtc gaagttgaag
      181 caaagttggt agagaatggt attctttatg aatttgttga accacaaaaa gaattttatg
      241 attttagcga agatattttg tctgatataa ttgcagaagg ctacgataaa gatgagattc
      301 ttgtagaatt taagaataga aaaaacaaaa tgcattcttc ctttagagat attgctgaag
      361 atacacttac taattctaaa gtaatgacta aagaggagtt ggcaacagaa attggcttat
      421 gaaatcgttc cttcaaaaca tgtcattaag tatttaaaaa agctaaaaga gaagccattg
      481 aaagaaaaat ttttgaattt aatttatgat gaaattgcga ttgatccata taagggtgaa
      541 caaaaaactg gggatttatc aggtatttgg tctagtgggt tcaaatatgc gggcacaaca
      601 tatagagtag cttacgagat tatagaaaac aaggttattc cagtattgtt atgtggaacg
      661 catgaaaact tttatgaaca gttgaaaaaa ttaatctagt gatttgttgt cgttcttaag
      721 aaggacggct tctttttatc gaaaatctga ttcgtctaca tgactaaagt cgcgagtatt
      781 ctctgcttct cgataaatta aataacgtca ttactttgag gaaagctata aatcaatata
      841 tttcagcttt tccttattta tgtactacct tttacttatt aattataagg tagtacatat
      901 atatcttttt ttggtagtaa ctaaaaataa atatgttata atagtatcaa atgaatattg
      961 ctaaaagaaa agaggtagga acgtggtagc gaagaaatat gtaatcggta attttaaagg
     1021 tggcgtaggt aaaagtacgt gcgcgcaaat gttagggttt gaatcagcag ttaatgaagg
     1081 aaaaagaacc ttgataattg acttggatat gcaaggaaat acttctgatg tgatgaactt
     1141 aacgcatatg aacttcacta acgaagaagg aggcggagaa ggtgaaccga ttgtttacga
     1201 aaatactgtg actgacgtgt tgataaacaa tcttccacct aaagaggctg tctataaagt
     1261 aatcaataac ctctatatac ttccagcaga catgtccttt gaaatgtatg atgattggat
     1321 caaagaccaa tataatgatt cattatccca attcaaatat atgaaagaaa agctagaacc
     1381 attatttgat gagtttgacg tcatctattt agatgtccca ccttctattt ctgtatatag
     1441 taaatccgcg atgtacattg cggactgggc aatcgtcgtc ttacagaccc aagtaaaatc
     1501 gatgagaaat gcgatgcaat atcttgagta tatggacttt tttgtgaaag aatttgatac
     1561 aaatttatat gttgctggtg ttattccttt catgttggaa agcggtgacg cagtagatca
     1621 ggaaatgtat aaacaagccc aagagattta tggggaacat ttgattaaaa atgttgtact
     1681 caaaaatgcg cgcttaaaac gatacgacgg atcaggtatt acgatggaga agacaaagaa
     1741 aggtctctta aaacaatggg atcgcaaaag ccacgaatta tttatcagta ttttgaaaga
     1801 attggaagaa cacgagaatt ggtatgcgga ggtggagtaa ttggctgaca tcaaaggact
     1861 tgatattttt gggaacaaca aaggactcgc caaaataaaa aaagaagcag aagcaaagaa
     1921 aaagcagcaa gcaattacac catctgaaaa taagcaatta gaagtgcacc aagttcaaga
     1981 aataaaaact tcctccaatg ttgaaagaca gagtagagga agaacaaaaa aagttggcgc
     2041 accaatcaga aagtttgata aggtttattc caccaagcat cctttaaaac tgtcgacttt
     2101 gttaaatgcg acttccagag ttttagtaga aaagtatgag actaatatga caagagatga
     2161 attgcttaga aaagctttag atgagtatat taaacagaat cttacgcaag aagataagca
     2221 agatttgttt aatgacgtgg tgaaagaact agacttgttc agagaaaaat atccaattgt
     2281 tccagaatta gatgatcgtg gagaaataat aagaactacc agtcagataa aacaagatac
     2341 cgaagactca ctgcgcaaag ggtggggaat gaataataaa gttagataat aatcaaagaa
     2401 gaactaagta actaccttta aacggtagtt acttagttct tctttgattt gcatgaaata
     2461 gaggggattt atttttctgg ttacgtgatt aaaaaatcca aaaaatgagc ataggaacgg
     2521 ttatgatttt agggtgtgtt ttaatgagat attgtgattt cattaacaag tgtatgataa
     2581 tctgttgttg agttagtaaa taagtaatag agctaactct aaaataaaac tattaggagg
     2641 agatgtaggt gaaaaagttt aaaaagaaaa tatatttagg attggttgtt ggggtgagta
     2701 tgtttgggtt catggctcat actaaacaag taactgcaga tactaaccaa aaagaaagga
     2761 ttatgagcca attagatgac tctcaaataa gtatgtaccc aatgagctaa ttgtcagttt
     2821 aaaggctaca caaagaattt caacagagaa aaataaaccg caaaatttat tgggtgtaaa
     2881 agttatggtg gaaaaagatt tgactcttag gacaaatgaa agcgattgta ataagcgaga
     2941 tcaa
//
LOCUS       DASHHB010000135         2929 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.145, whole genome shotgun sequence.
ACCESSION   DASHHB010000135 DASHHB010000000
VERSION     DASHHB010000135.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2929)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2929)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2929
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.145"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..493
                     /locus_tag="U2K71_002837"
     CDS             <1..493
                     /locus_tag="U2K71_002837"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol phosphate phosphatase"
                     /protein_id="HEM7316838.1"
                     /translation="LVSIHQNGRFDYMDDVVLTKDPIQTTKEYFDQMLQVLTDFPEGD
                     ILTHFDYGLRRFTFTAEEFAEHFEAQLLTIFKKVVERELAMELNAKSFLKYGNEDLYR
                     YAVPLYQSVGGKLFTLGSDAHVAEDYQLGFKKMSQLLADCQVEKLLVIQGKERRLTPL
                     PIL"
     gene            526..909
                     /locus_tag="U2K71_002838"
     CDS             526..909
                     /locus_tag="U2K71_002838"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrolase"
                     /protein_id="HEM7316839.1"
                     /translation="MSNLWDYNQEAPIHYLIARHWDALKIEAVCRSLLAAVPKQQLEN
                     FLVADSLQREKVQAYFAAFKDQPLEYLHAQFHLFYQVAAPDDYNDLRGQLQLTFQADE
                     TAYTVLLGMARLGDQAKVEWRIFDI"
     gene            985..1116
                     /locus_tag="U2K71_002839"
     CDS             985..1116
                     /locus_tag="U2K71_002839"
                     /inference="COORDINATES: protein motif:HMM:NF012387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM7316840.1"
                     /translation="MLVTDAGEIRLRLFPDQAPLAVANWRSLAEQGVYDNTPFGSCH"
     gene            1085..1321
                     /locus_tag="U2K71_002840"
     CDS             1085..1321
                     /locus_tag="U2K71_002840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HEM7316841.1"
                     /translation="MTTHLSARVIKEFVIQGGALDGSGDEAVSSYEGFFADEVHRGLY
                     HFNGALALGDHGPHTNGNQFFIVQNTKAQADLLM"
     gene            complement(1399..2370)
                     /locus_tag="U2K71_002841"
     CDS             complement(1399..2370)
                     /locus_tag="U2K71_002841"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002361027.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HEM7316842.1"
                     /translation="MEGTVLRIEQGSLHDGAGLRTVVYLKGCPLRCAWCSIPESQSKQ
                     IEKGFGQTMTAEEVMDEIEKDAVFYFHSDGGVTISGGEALVQADFAKEILQKSKYIGI
                     NTVLETSFCGAYNEIQKVAPYVDTLFVDVKMFTSKLHKQWTGLDNQQILKNIRRFLIE
                     YPNCEVRIRVPVVPGINMNLTELLTIACFVADLDRFVPLELLPYHCYGMHGYQALGLE
                     YPLADTPAPSPKEMFALADQLARTVPHLLVTALTKTFIYLLNPSFILWINERHSLAAA
                     SSRIELLLKSVSVFLRCFFEVNLSNEHWLNRYIFTVCRLSCDRCYDF"
BASE COUNT          859 a          613 c          609 g          848 t
ORIGIN      
        1 gctggtaagt attcatcaaa atggccgctt tgattatatg gacgatgtgg ttttaaccaa
       61 agatcccatt caaactacca aggaatattt tgatcagatg ttgcaggtct tgacggattt
      121 tccagaagga gatattttga ctcactttga ttatggatta cgccgcttca ccttcacagc
      181 ggaagaattc gcggagcatt ttgaggctca gctgctaacg attttcaaaa aggttgttga
      241 acgagaattg gcgatggagt taaatgccaa gagcttcttg aaatacggca acgaagacct
      301 ttaccgttat gccgttcctt tgtatcaaag tgtcggcgga aagctcttta ccttaggctc
      361 ggatgctcat gttgccgaag attatcagct gggctttaaa aaaatgagtc agctgttggc
      421 agactgtcag gtagaaaagt tgctagtcat tcaaggaaaa gaacgccgcc tgacgccgct
      481 gccgattttg tgattcctat ataatagaag aaagtggtga aaaaaatgag caacctgtgg
      541 gactacaatc aagaggcacc catccattat ttaattgcgc gacattggga tgcgttgaaa
      601 attgaggcgg tttgccgatc cttgttagca gccgttccaa aacaacaact ggaaaatttt
      661 ttggtggctg acagcttgca gcgggaaaaa gttcaggctt attttgcagc ctttaaagat
      721 cagcctttgg aatatttgca tgcgcagttt catctttttt atcaagtagc cgccccagat
      781 gactacaacg atttacgagg acagctgcaa ttgacctttc aagcggatga aaccgcctat
      841 accgttttat tgggaatggc tcgccttggt gatcaggcga aggtcgagtg gcggattttc
      901 gatatttgat ggcaggatca aggcgtttca gttgcaaacc ttcctctctt tcaattagaa
      961 aaaccgcagc aggaagaagt ttgtgtgctg gtcaccgatg ctggtgaaat tcgtctccgc
     1021 ttatttcctg accaagctcc cctggctgta gccaactggc gctccctagc tgagcaaggc
     1081 gtttatgaca acacaccttt cggctcgtgt cattaaggaa tttgttattc aaggtggcgc
     1141 tttggatggt tctggtgatg aagctgtttc cagctatgag ggctttttcg ctgatgaagt
     1201 gcatcgcgga ctttatcatt ttaatggcgc cttggcctta ggggaccacg gcccccacac
     1261 caacggcaac cagtttttta ttgtgcaaaa cacaaaggcg caggctgacc tgctaatgta
     1321 aagcaagcat acttggagct tggaggacta ccggagctgg atggtcgtta tactgttttt
     1381 ggacaggtat accaagggtt agaaatcgta gcagcgatcg cagctcagcc gacaaacagt
     1441 gaagatgtac cgattgagcc agtgttcatt cgacaaatta acttcgaaaa aacatcgtaa
     1501 aaagacactc actgacttca gcaaaagctc tattcttgag ctggctgcag ctagtgagtg
     1561 tctttcattt atccataaga taaagcttgg attcaataaa taaataaatg ttttggttaa
     1621 agctgtgacc agcaaatgag gaaccgtgcg agccagttga tctgccaatg caaacatttc
     1681 tttaggtgaa ggcgctggcg tgtctgccaa cggatactcc agtcccaatg cttggtaacc
     1741 gtgcatacca tagcagtggt acggtagtaa ttctaacggt acgaaccgat caagatctgc
     1801 cacaaaacaa gcaatcgtca gcagctcggt caggttcata ttgatacctg gaaccacagg
     1861 aactcgaatt cgcacttcgc agttaggata ctcaattaaa aagcgacgaa tattcttcaa
     1921 aatttgttgg ttatctaagc cggtccattg tttgtggagc ttgctggtaa acatttttac
     1981 atccacaaac aatgtatcca catagggagc taccttttgg atttcattat aagcaccaca
     2041 aaagctggtt tccagtacgg tattgattcc aatgtatttc gatttttgca aaatttcttt
     2101 agcaaaatcc gcctgaacca gtgcctcgcc tccgctaatg gtcactccgc catccgagtg
     2161 aaagtaaaaa acagcatcct tctcaatctc atccattacc tcttcagcgg tcatggtctg
     2221 gccaaatcct ttttcaatct gcttgctttg tgattcggga atcgaacacc aggcacagcg
     2281 taatgggcag ccctttaaat aaacaacggt ccgtaaacca gcaccatcat gtagagaacc
     2341 ctgttctatt cttaatactg ttccttccat atagattttt gaattacaca ctagtttaaa
     2401 ctaaaacaca caaggaaaaa cttaccattt aatcataaaa ttagctcata taaatatact
     2461 aggatgtaaa atagactggt caaaatctgg tcaaaaaaga ggactaatac aggtagtcct
     2521 cttttttgtt attttaatat tctaaatcat tttttattgg tttattccct tattcgatgt
     2581 agactcaaat cactatatca ctagatatca aacggcgtca tacggaatca aaaacatcaa
     2641 atttgacctg ttaaatggtc attgaataac attcaaaatc aataaaacgg agtcagacac
     2701 ggagtcaaaa acgctgagag taaaaaatat atacagcctt gtaggtaaag taccacaggg
     2761 ctgtatattt tttatttcag attaccttaa atgattccat ttacttttta tctcattttt
     2821 tcctactagt tagtatcaaa tactctccta ttttctcaat aaactatttt tggatacatg
     2881 gtatctgaaa cagctaaaac cttgtcatta cagcaaaaaa tcacgttat
//
LOCUS       DASHHB010000136         2918 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.146, whole genome shotgun sequence.
ACCESSION   DASHHB010000136 DASHHB010000000
VERSION     DASHHB010000136.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2918)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2918)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2918
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.146"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            205..1173
                     /locus_tag="U2K71_002842"
     CDS             205..1173
                     /locus_tag="U2K71_002842"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEM7316843.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1166..2197
                     /gene="vanA"
                     /locus_tag="U2K71_002843"
     CDS             1166..2197
                     /gene="vanA"
                     /locus_tag="U2K71_002843"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEM7316844.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2203..2811
                     /locus_tag="U2K71_002844"
     CDS             2203..2811
                     /locus_tag="U2K71_002844"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEM7316845.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          863 a          559 c          708 g          788 t
ORIGIN      
        1 taatttttta ggaaaatctc aaggttatct ttactttttc ttaggaaatt aacaatttaa
       61 tattaagaaa cggctcgttc ttacacggta gacttaatac cgtaagaacg agccgttttc
      121 gttcttcaga gaaagatttg acaagattac cattggcatc cccgttttat ttggtgcctt
      181 tcacagaaag ggttggtctt aattatgaat aacatcggca ttactgttta tggatgtgag
      241 caggatgagg cagatgcatt ccatgctctt tcgcctcgct ttggcgttat ggcaacgata
      301 attaacgcca acgtgtcgga atccaacgcc aaatccgcgc ctttcaatca atgtatcagt
      361 gtgggacata aatcagagat ttccgcctct attcttcttg cgctgaagag agccggtgtg
      421 aaatatattt ctacccgaag catcggctgc aatcatatag atacaactgc tgctaagaga
      481 atgggcatca ctgtcgacaa tgtggcgtac tcgccggata gcgttgccga ttatactatg
      541 atgctaattc ttatggcagt acgcaacgta aaatcgattg tgcgctctgt ggaaaaacat
      601 gatttcaggt tggacagcga ccgtggcaag gtactcagcg acatgacagt tggtgtggtg
      661 ggaacgggcc agataggcaa agcggttatt gagcggctgc gaggatttgg atgtaaagtg
      721 ttggcttata gtcgcagccg aagtatagag gtaaactatg taccgtttga tgagttgctg
      781 caaaatagcg atatcgttac gcttcatgtg ccgctcaata cggatacgca ctatattatc
      841 agccacgaac aaatacagag aatgaagcaa ggagcatttc ttatcaatac tgggcgcggt
      901 ccacttgtag atacctatga gttggttaaa gcattagaaa acgggaaact gggcggtgcc
      961 gcattggatg tattggaagg agaggaagag tttttctact ctgattgcac ccaaaaacca
     1021 attgataatc aatttttact taaacttcaa agaatgccta acgtgataat cacaccgcat
     1081 acggcctatt ataccgagca agcgttgcgt gataccgttg aaaaaaccat taaaaactgt
     1141 ttggattttg aaaggagaca ggagcatgaa tagaataaaa gttgcaatac tgtttggggg
     1201 ttgctcagag gagcatgacg tatcggtaaa atctgcaata gagatagccg ctaacattaa
     1261 taaagaaaaa tacgagccgt tatacattgg aattacgaaa tctggtgtat ggaaaatgtg
     1321 cgaaaaacct tgcgcggaat gggaaaacga caattgctat tcagctgtac tctcgccgga
     1381 taaaaaaatg cacggattac ttgttaaaaa gaaccatgaa tatgaaatca accatgttga
     1441 tgtagcattt tcagctttgc atggcaagtc aggtgaagat ggatccatac aaggtctgtt
     1501 tgaattgtcc ggtatccctt ttgtaggctg cgatattcaa agctcagcaa tttgtatgga
     1561 caaatcgttg acatacatcg ttgcgaaaaa tgctgggata gctactcccg ccttttgggt
     1621 tattaataaa gatgataggc cggtggcagc tacgtttacc tatcctgttt ttgttaagcc
     1681 ggcgcgttca ggctcatcct tcggtgtgaa aaaagtcaat agcgcggacg aattggacta
     1741 cgcaattgaa tcggcaagac aatatgacag caaaatctta attgagcagg ctgtttcggg
     1801 ctgtgaggtc ggttgtgcgg tattgggaaa cagtgccgcg ttagctgttg gcgaggtgga
     1861 ccaaatcagg ctgcagtacg gaatctttcg tattcatcag gaagtcgagc cggaaaaagg
     1921 ctctgaaaac gcagttataa ccgttcccgc agacctttca gcagaggagc gaggacggat
     1981 acaggaaacg gcaaaaaaaa tatataaagc gctcggctgt agaggtctag cccgtgtgga
     2041 tatgttttta caagataacg gccgcattgt actgaacgaa gtcaatactc tgcccggttt
     2101 cacgtcatac agtcgttatc cccgtatgat ggccgctgca ggtattgcac ttcccgaact
     2161 gattgaccgc ttgatcgtat tagcgttaaa ggggtgataa gcatggaaat aggatttact
     2221 tttttagatg aaatagtaca cggtgttcgt tgggacgcta aatatgccac ttgggataat
     2281 ttcaccggaa aaccggttga cggttatgaa gtaaatcgca ttgtagggac atacgagttg
     2341 gctgaatcgc ttttgaaggc aaaagaactg gctgctaccc aagggtacgg attgcttcta
     2401 tgggacggtt accgtcctaa tcgtgctgta aactgtttta tgcaatgggc tgcacagccg
     2461 gaaaataacc tgacaaagga aagttattat cccaatattg accgaactga gatgatttca
     2521 aaaggatacg tggcttcaaa atcaagccat agccgcggca gtgccattga tcttacgctt
     2581 tatcgattag acacgggtga gcttgtacca atggggagcc gatttgattt tatggatgaa
     2641 cgctctcatc atgcggcaaa tggaatatca tgcaatgaag cgcaaaatcg cagacgtttg
     2701 cgctccatca tggaaaacag tgggtttgaa gcatatagcc tcgaatggtg gcactatgta
     2761 ttaagagacg aaccataccc caatagctat tttgatttcc ccgttaaata aacttttaac
     2821 cgttgcacgg acaaactata taagctaact ctttcggcag gaaacccgac gtatgtaact
     2881 ggttcttagg gaatttatat atagtagata gtattgaa
//
LOCUS       DASHHB010000137         2888 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.147, whole genome shotgun sequence.
ACCESSION   DASHHB010000137 DASHHB010000000
VERSION     DASHHB010000137.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2888)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2888)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2888
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.147"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            89..406
                     /locus_tag="U2K71_002845"
     CDS             89..406
                     /locus_tag="U2K71_002845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SdpI family protein"
                     /protein_id="HEM7316846.1"
                     /translation="MLFYSNFILIVAILLLLNIWIFDRSRNSSIGFRTKRSLSSKKNW
                     VYSQTIFYGGIVLISLLSSTLYSLNIIDVSTSNSISIIGIIIAAIITQLFLVFGEKKR
                     SKK"
     gene            complement(497..862)
                     /locus_tag="U2K71_002846"
     CDS             complement(497..862)
                     /locus_tag="U2K71_002846"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316847.1"
                     /translation="MIKTCWKNLPLLLSFVPYVHFALLLDFRYHSVSGFITLIFLSLF
                     AGYYFQRNRRIISLFIANIISTVTSYLFCANFTEWRYFYHPLKPTQLILLLAGIYLVP
                     QILGSLWAVALSYKKARHP"
     gene            1070..1552
                     /locus_tag="U2K71_002847"
     CDS             1070..1552
                     /locus_tag="U2K71_002847"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316848.1"
                     /translation="MRRKAVIDRLYLFFWFFPIVLLPFTWVNAVVQGAEILGLIQGSG
                     FVVSRNFPALIVFYLVISVGLLLPKSLINHYNWQLLYFSCLLVFTSTFSLLPRLIIGP
                     NGLSESGLKDLAYSSMYFLTIKPWFYLVIASFILATFFYFWTEIRIKSERSVSIKNNY
                     "
     gene            1749..2639
                     /locus_tag="U2K71_002848"
     CDS             1749..2639
                     /locus_tag="U2K71_002848"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002289807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS family protein"
                     /protein_id="HEM7316849.1"
                     /translation="MKKVLFTLGMMGILLAGCGSGSGNTDGSATQNNSSDSNEQVKIV
                     AVGSTALQPLVDAAQESFVQENPNYQISVQGGGSGTGLSQVEAGAVTIGNSDVFAEER
                     DGVDASKLVDHKVAVVGMAPIVNKDTDVKDITKQELIDIFTGKITNWKEVGGKDQKIN
                     VVNRANGSGTRATFEKWGLDGATPVQSQEQDSSGTVRQLVSQTPGAISYLAFSYLDDS
                     TQALSIDGVEPKEENVADNSWGIWSYEHMYTNGKPSPEVQKFLDYMMTEEIQEGPVKE
                     LGYLPITMMEVERDHEGNIK"
BASE COUNT          996 a          446 c          551 g          895 t
ORIGIN      
        1 ttttaaaaca tcacaaggaa aaacttaact atggtattat ctaaacataa ttttttcggt
       61 atttaatatt taattaggag gtaatatttt gcttttttat agtaatttta tcttaatagt
      121 agcgatatta ttacttctga atatatggat ttttgatagg agtagaaatt caagtatagg
      181 ttttagaact aaacgaagtt tgtcttctaa aaaaaattgg gtatactcac aaactatttt
      241 ttatggagga attgttttaa ttagtttact ttcttcaaca ctttattctt tgaatattat
      301 agatgtttcc acctcaaatt caatatctat tattggaatc attattgctg caattattac
      361 acaactgttt ttagtgtttg gagagaagaa aagaagtaaa aaatagaaaa actagtgata
      421 gttttggtga tataagaaaa acatatatgt ataaaaagaa gaaagtagct tctctttgta
      481 catatatggt ttgtttttat ggatgccgag cttttttata agataaggct accgcccata
      541 acgacccaag gatttgtgga actaagtaaa tacctgcaag aagcaagatc agctgggtcg
      601 gttttaaagg atgatagaag taacgccatt cagtaaaatt ggcgcaaaat aaataggaag
      661 tgacagtaga tataatattg gcaatgaata aagatataat acgcctgttc ctttgaaaat
      721 aatatccagc aaaaagagat aaaaatatca atgtgatgaa cccgcttacg gaatgataac
      781 gaaaatcaag cagtaaggcg aaatgcacat aaggaacaaa agataaaagc aacggaaggt
      841 ttttccagca agtttttatc atctttcatc accacctaac aagtgttatt attttgttag
      901 ttttattgta aattgaatcc tgcaataatc aattactttt tcattatttc tagattaaat
      961 gaataaactc tcatgccatt atctctaaaa aaaaagtttt tccccctaat ttttttagat
     1021 agaaaattgt atagtatgat gagagaaaaa gtagtttggg ggaacgttaa tgagaagaaa
     1081 agcagtaatc gatcggcttt atttattttt ttggtttttt cctatcgttt tgctaccttt
     1141 tacatgggta aatgctgttg tacaaggtgc ggagatatta ggcctgatcc aaggaagcgg
     1201 atttgtggtt tctaggaatt ttccagcttt gattgttttc tatttagtga taagtgtagg
     1261 tctcttatta ccaaaatctc tgatcaatca ctacaattgg cagctgcttt acttttcttg
     1321 cttattagta tttacatcta cgttttcttt gctgcctcgc ttgattatcg gccctaatgg
     1381 tttgagcgag agtggattga aggatttagc ttattcctcc atgtatttct tgacgatcaa
     1441 accatggttc tatttagtaa ttgcctcatt tattcttgca acattttttt acttctggac
     1501 agaaatcagg ataaaaagtg aacgatctgt atctataaaa aataactatt gatggtggat
     1561 catcagtaaa aaaacatatg agctgcgtaa aatgcatgtt tctcatatgc tttttttatt
     1621 attatgtaaa gaatgcgaaa aacgtttaca tgatttttac ataacttaac tatattttta
     1681 cagcaaccat acgagggatt tacagtcgac tgataaactt tagttgtaaa taagaaagga
     1741 gcaataaaat gaaaaaagta ttgtttactc taggtatgat gggaattctt ttagccggat
     1801 gcggctcagg atcaggaaat acagatggct ctgccacaca aaataattca agtgactcaa
     1861 atgaacaagt aaaaatcgtt gcggtaggtt cgacagcatt acagccgtta gtcgatgcag
     1921 cacaagaaag ctttgtacaa gaaaatccaa attatcaaat ctctgtacaa ggaggaggaa
     1981 gcggaactgg tttgagccaa gtcgaagctg gagcagttac tatcggaaac tctgatgtat
     2041 ttgcagaaga aagagacgga gttgatgctt caaaattagt ggatcataaa gtagcagtgg
     2101 tcgggatggc accaatcgtc aataaagata cagatgtcaa agatatcacc aaacaggaat
     2161 tgattgatat ctttacagga aaaatcacta actggaagga agttggcggg aaagatcaaa
     2221 aaatcaacgt cgtgaaccgt gcaaacggca gcggtacccg cgcgacattc gaaaaatggg
     2281 gactggatgg tgctacccct gtgcagtccc aagaacaaga ttcatcagga acagttcggc
     2341 agctggtaag tcaaacacca ggagcaatca gctatttagc tttctcatat ctcgatgatt
     2401 ccacacaagc actaagtatc gacggtgtag aaccaaaaga agagaatgta gcagataaca
     2461 gctggggaat ctggtcatat gaacatatgt acacgaacgg aaaaccttcc cctgaagtcc
     2521 aaaaattctt agactacatg atgacagaag aaatccaaga aggacctgtc aaagaattgg
     2581 gctatttacc aatcacaatg atggaagtag aacgtgatca cgaaggaaat atcaaataac
     2641 tagcagataa acatgccacg gtagtcgtta ctattaagga aaaatcaact atgtttcccc
     2701 acatatacat ttttccttta attgtgaaaa atagttcctc atattttgcc gttttttgag
     2761 ctaataccga gggtcagctg attgaacacc gtttattcga aaaaaagagc tacgacatag
     2821 aactgtcgta actttttttg gctcttcgtc aaatcgtgtt ggtgaagaca aatttgaata
     2881 aaacttag
//
LOCUS       DASHHB010000138         2851 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.148, whole genome shotgun sequence.
ACCESSION   DASHHB010000138 DASHHB010000000
VERSION     DASHHB010000138.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.148"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1412
                     /locus_tag="U2K71_002849"
     CDS             <1..1412
                     /locus_tag="U2K71_002849"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HEM7316850.1"
                     /translation="PTVEGEKVEVGGTVDLTDNVTNLPALPEGTTVTDVTPGGTIDTN
                     TPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSDADKYEPTVEGEKVEIGGTVDLTDNV
                     TNLPALPEGTTVTDVTPGGTIDTNTPGNYEGVIEVTYPDGTKDTVKVPVEVTDNRSNA
                     DKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYAWKEKVDVSTAGNKKGTIVVTY
                     PDGSSDEVEVDVTVTDTRSDADKYNPEGQKVTTDLNKEPDASEGIKNKEDLPKGTTYT
                     WKEKVDVSTAGNKKGTVVVTYPDGSKEEVEVTISVVDKKAPNKPQVDPITEGDQIVTG
                     KTEPNAEVTVTLPDGSQHHGTADKNGNFTVKVPKLEAGTKVIVTATDESGNTSEPTNV
                     VVSSNKKDSGKNGSKGSKTDNQGSNSNQDKNRGKSQSSKVFPKTGESDSNIFTISGGL
                     ILLGTLGLLGYKNRKKENE"
     gene            1546..>2851
                     /locus_tag="U2K71_002850"
     CDS             1546..>2851
                     /locus_tag="U2K71_002850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HEM7316851.1"
                     /translation="MEKIVIIGSNHSGLAAARFLNLSDSNYEVILIDKKSEISYLECG
                     TSLFIKGEVSNLEDLFYQNRMTLNSEIKKLFLETEVDKIDIQNKKISCVKKDGTRFSQ
                     EYDKVILATGSKQIMLDIPGNNLANIFSIKNFPNAKEIFKALDDSTIKKVGIIGAGYI
                     GVEIAEAIRKRGKEVYLFDVADRVLSTYYDRSFSDKVEEILSKNGIHLCLSESPKKYL
                     GRKKIEKIVTTSGTQYSMDMVVQAIGFLSNSPIGEKELLRDVSGAYLTNEYQQTNIKD
                     IYAIGDCATTFFTSINRSSVDFSISNALRTAYIATQHINNQDFTPSGSQFTNGFSIFN
                     YNFYAAGLNLKTARLLSESIDYIEIEDFIRPAFLRKNSRVKLRISFEKNTKRIVGVQL
                     CSQEDLSGLLSMFSLAIEEQITLDKLKMLDYLFHPSFSQPYNF"
BASE COUNT         1118 a          391 c          559 g          783 t
ORIGIN      
        1 aaccaacagt agaaggcgaa aaagtagaag ttgggggcac agtagatcta acagataatg
       61 ttactaactt gccagcgtta ccagaaggaa caacagtaac agatgttact cccggcggta
      121 caattgatac caacacacca ggtaattacg aaggtgtcat tgaagtaacg tatccagacg
      181 gtacgaaaga tacggtgaaa gttccagtag aagtgacaga caatcgttct gacgccgata
      241 aatatgaacc aacagtagaa ggcgaaaaag tagaaattgg aggcacagta gatttaacag
      301 ataatgttac taacttgcca gcgttaccag aaggaacaac agtaacagat gttactcccg
      361 gcggtacaat tgataccaac acaccaggca attatgaagg tgtcattgaa gtaacgtatc
      421 cagacggtac gaaagatacg gtgaaagttc cagtagaagt gacagacaat cgttctaatg
      481 cagataaata caatcctgaa ggacaaaaag tgactacaga cttaaataaa gagccagatg
      541 catctgaggg aattaagaac aaagaagatt taccaaaagg aactacttat gcttggaaag
      601 agaaagtaga tgttagcaca gctggaaata aaaaaggtac tattgtagtg acatatccag
      661 atggatcatc tgatgaagtt gaagtagacg ttacagtgac agatactcgt tccgatgcag
      721 ataaatataa tcctgaagga caaaaagtga ctacagactt aaataaagag ccagatgcat
      781 ctgagggaat taagaacaaa gaagatctac caaaaggaac tacttatact tggaaagaaa
      841 aagtagatgt tagtacagct ggaaacaaaa aaggtactgt agtagtaaca tatccagatg
      901 gatctaaaga agaagtagaa gttactattt ctgtagtaga taaaaaagca ccgaataaac
      961 ctcaagttga tcctattaca gagggtgacc aaattgttac tggtaaaact gaaccaaatg
     1021 cagaggtgac agttacatta ccagacggaa gtcaacacca tgggacagct gataaaaatg
     1081 gtaattttac agttaaagtt cctaaattag aggcagggac aaaagtgata gtaactgcaa
     1141 ctgatgaatc tggtaatact agtgaaccta ctaatgtagt tgtttcttcc aacaaaaaag
     1201 acagtggaaa aaacggtagt aaaggtagta agactgacaa tcaaggtagc aacagcaatc
     1261 aagacaaaaa tagaggcaaa agccagtcct caaaagtttt tcctaagacg ggagagagtg
     1321 acagtaatat ctttactatt agcggtggtt taatactgtt aggaacgtta ggattattag
     1381 ggtataaaaa tcgaaaaaaa gagaatgaat agaaaatcaa tttcttaagt cttctctgag
     1441 aagaggaaga atctttttct tcctcttttt gcttttttat gatgggaaat aagtgttagt
     1501 taaagatgaa tatacttttt atagaaagga aaagaatgta attaagtgga aaaaatagta
     1561 attattggtt caaaccattc agggctagcc gcagctcgat ttctaaactt aagcgattca
     1621 aattatgaag ttattctaat cgataaaaaa tcagaaatta gttacttgga atgtgggaca
     1681 tcgttattta taaaaggaga agtaagtaat ttagaagatc ttttttatca aaatagaatg
     1741 acactaaatt ctgagataaa aaagttattt ctagagacag aagttgacaa aattgatatt
     1801 caaaataaaa agattagttg tgtaaaaaaa gatggtacaa gattctctca agaatatgac
     1861 aaagttatat tagctacagg ttcaaagcaa attatgctag atataccggg gaataattta
     1921 gctaatattt tttcgataaa gaattttcca aatgcaaaag agatttttaa agctttggat
     1981 gatagtacta ttaaaaaagt aggaattatt ggagcaggat atattggagt tgaaattgct
     2041 gaagccataa gaaaaagagg gaaagaagta tatcttttcg atgttgcaga tagagtgttg
     2101 tcgacttatt acgaccgtag tttttcagat aaagttgaag aaatactatc aaaaaatgga
     2161 atacatcttt gtctatcaga aagtcctaaa aaatatttag gaagaaaaaa gattgaaaaa
     2221 atagtcacta caagtggtac ccaatactct atggatatgg tcgtacaagc tataggattt
     2281 ctatctaata gtccaattgg ggaaaaggaa ctcttgagag atgttagtgg agcatatcta
     2341 actaatgaat accagcaaac aaatataaaa gatatatatg ctattgggga ttgtgctaca
     2401 acttttttta cgtccattaa tagaagtagt gttgattttt ctatttctaa tgctctaaga
     2461 acagcttata ttgcaactca acatattaac aatcaagatt ttacaccttc tggttcccaa
     2521 tttacaaatg gcttttctat atttaattac aatttttacg cagcaggttt gaatttaaaa
     2581 acagcacgat tattaagtga gtctatagat tatatagaaa tagaagattt cattagacct
     2641 gcttttttga gaaaaaatag tcgtgtaaaa ttaagaatta gctttgaaaa aaatacaaag
     2701 agaatagtag gtgtacaact ttgtagtcaa gaagatttat ctggactatt atcaatgttt
     2761 tctcttgcta tagaagaaca aattacatta gacaaattga aaatgttaga ttatttattt
     2821 catcctagtt ttagccaacc ctataatttt a
//
LOCUS       DASHHB010000139         2847 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.149, whole genome shotgun sequence.
ACCESSION   DASHHB010000139 DASHHB010000000
VERSION     DASHHB010000139.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.149"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            939..1907
                     /locus_tag="U2K71_002851"
     CDS             939..1907
                     /locus_tag="U2K71_002851"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="replication initiation protein"
                     /protein_id="HEM7316852.1"
                     /translation="MSNEIVKYHHELNTIPLRKFTPVEMNLFFSIVSRMRDVGDKKVQ
                     FTFEQLKDLSNYKATANVRFIDDLETTYDKLMDLRFGRRSADGLQRERFVLFNQFKID
                     GKADIPFAEIQVHEKALPLLNNLEEWVRYSLQQFNELESSYSKTMFRLLKQYRTKGFA
                     YFSKEDFHELLDIPKSYNQGTIDQRVFNPIRQELTAIFKGLTIKKKYGKGRGKPVIGY
                     QFTFKPEIKKADDFNKHAPTKEEQNRALELANRQTQHEKETTSLQERIKEQKRKLELL
                     QQLEELEKQQKNRENETDKQKLDNNSDIPLELRKDLLDNLQNLFKD"
     gene            1917..2387
                     /locus_tag="U2K71_002852"
     CDS             1917..2387
                     /locus_tag="U2K71_002852"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF536 domain-containing protein"
                     /protein_id="HEM7316853.1"
                     /translation="MTDKSIKEFAEELGVSKQAIRKYFDKLPTKLTPKLIGGKYVISA
                     KAQEFIKNSINQINEVDTEVDSLVDSILIAELKEKNKQIDHLQGVIEIQQKLLDQQQQ
                     LTLQANQQIDKLQKRLQLVYTEETSENESTTLSEETESIEKQTKKKQKWWKLWK"
     gene            2403..2573
                     /locus_tag="U2K71_002853"
     CDS             2403..2573
                     /locus_tag="U2K71_002853"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316854.1"
                     /translation="MTRKKLIFGSILLGILGFSPMLLSVINKGNKHGELKLNPNYYNS
                     PNEELFVKETDK"
BASE COUNT         1101 a          471 c          492 g          783 t
ORIGIN      
        1 agtgacctta taggcactct cacattacga acgatacgtc cctcggacgt actgaaaggg
       61 tgggacttat gagcgaacta atgcaaaatt tggctagcaa aaaagcgaaa aaatatatgc
      121 taataatcga gcgaaatgaa cactttttca agtgagcgaa tccgccatga caaagctgaa
      181 acagtttatt tcagctttgt catggcgtgt gccgactttt ttaaaagtct aggcacacga
      241 gcaaatatct ttatcaagct ctaatttgcg ttttaaggcg tttcaaatgt ctaagtatat
      301 aaatatataa gaatagagtt aagagtgctt aaatcgaatt ttagagggaa ttcagacaat
      361 tttagggttg ttagggcttg ccctgaccgc ctgtaaggcg ctttaagaac aagataaggg
      421 atttatacta agactgtctt aaaacgtctc aaacgaaggc tcatagcttc tcaaagagtt
      481 ccttatgaac agttttagca aaggagccgg aggcgactgc gcagtataat gtgagagcag
      541 agcgatcaca cctttttaag caactatagg gagtaagatt cccagtttag aaaaatgcgc
      601 gaatctgttt ggagaaaaaa gaagtggttt tttaggcggg aaattttgtc cccctctttt
      661 ataatctctt tttaaacctc tttttaaacc tctttaccgt cggctgtatc cttactctcc
      721 caagggaatg acgcttttat ctacatcgtt ttgtctgttt atctacatcg ttttgtctgt
      781 ttatctacat cgttttgtct gtttatctac atcgttttgt ctgtttgtac attgaattag
      841 tacatcgacg atgttaatat aagatgtaga taaacagaca ttttgtattt cataacgtct
      901 gtatctcttg tgcgagtaag aaaggagcta ttttaaatat gtctaatgaa atcgtaaagt
      961 atcatcatga attaaataca attccgttgc gaaaatttac accagtcgaa atgaatttgt
     1021 tcttctctat cgtatcaaga atgcgtgatg taggtgataa aaaagtacaa tttacattcg
     1081 agcaattaaa agatttaagc aattataaag caactgcaaa tgttcgattc atagatgatt
     1141 tagaaacaac gtatgacaaa ttaatggatt tacgttttgg tagaagaagt gctgacggtt
     1201 tgcaacgtga acgttttgtt ttatttaatc aatttaaaat cgacggaaaa gctgatattc
     1261 cttttgctga aatacaagtt catgaaaaag ccttaccctt acttaataat cttgaagaat
     1321 gggtgcgcta ttctctccaa caattcaatg aattagaaag cagttattca aaaacaatgt
     1381 ttcgattact aaaacaatac agaaccaaag gtttcgctta tttttcaaaa gaagactttc
     1441 atgaattact tgatattcct aaaagttaca accaaggtac gatcgatcaa cgtgtgttca
     1501 atccaatacg acaagaacta accgcaatct ttaaaggttt aacaatcaaa aagaaatacg
     1561 gaaaaggccg tgggaagcct gtcattggct accaattcac atttaaacca gaaatcaaaa
     1621 aagcagacga ctttaacaaa catgcaccaa ccaaagaaga acaaaatcgg gctttagaac
     1681 tagctaatcg acaaactcaa cacgaaaaag aaactacttc attacaagaa agaattaagg
     1741 aacaaaagcg aaaattagaa cttttacaac agttagaaga gttagaaaaa caacaaaaaa
     1801 atagagaaaa cgaaacggat aaacaaaagt tagacaacaa ttccgatatt cctctagaac
     1861 taagaaaaga cttacttgat aatcttcaaa acttatttaa agactaagga gtattcatga
     1921 cagataaatc aataaaagaa tttgcagaag aactaggtgt cagcaaacaa gccattagaa
     1981 agtattttga taaactgcca accaagttga cacctaagtt gataggtgga aaatacgtaa
     2041 tatcagcaaa agctcaagag tttataaaaa atagtatcaa ccaaataaat gaagttgata
     2101 ctgaagttga cagtttagtt gatagtattt taattgcaga attaaaagaa aaaaacaaac
     2161 aaattgatca tttacaaggg gtcatagaaa tacaacaaaa attactcgac cagcaacaac
     2221 aactcacctt gcaggcaaat caacaaatcg acaaactaca aaaacggtta caattagttt
     2281 atacggaaga aacttcagaa aatgaatcaa ctactctttc tgaagaaaca gaatcgatag
     2341 aaaaacaaac aaagaagaag caaaaatggt ggaaactatg gaaataactg gaggaaacat
     2401 tcatgacaag aaaaaagctc atatttggtt ctattttact aggaattcta ggcttcagtc
     2461 cgatgttatt gtcagtgatc aataaaggaa ataaacatgg tgaattgaaa ttgaatccaa
     2521 attattacaa cagtcctaac gaagagttat ttgtaaaaga aacggataaa taataaaaaa
     2581 agactgctct aacgagcagc ccaaaatgag attatcctag tttagcaaca taaaccattt
     2641 attagatgaa aatgagatac aaacaaagcg tatcaaatcc tctcaaaaat aacaagtctt
     2701 taaaaaatga cagataaaaa tgcacaaaga attagatata gataaccgcg aaaaatcaag
     2761 ccttaaaaag agctttttac ctagaccaat ccaaaatcac aaagtgagcc tctgtatcgg
     2821 ttgatacaag ggctacttgg catagtc
//
LOCUS       DASHHB010000140         2778 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.150, whole genome shotgun sequence.
ACCESSION   DASHHB010000140 DASHHB010000000
VERSION     DASHHB010000140.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2778)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2778)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2778
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.150"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..197
                     /locus_tag="U2K71_002854"
     CDS             <1..197
                     /locus_tag="U2K71_002854"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316855.1"
                     /translation="WFLSNAQSLVLLAIVVIGLYLGFKREFSKLIGFLIIAIIAVGLV
                     FNAAGVKDILLELFNRIIGA"
     gene            314..811
                     /locus_tag="U2K71_002855"
     CDS             314..811
                     /locus_tag="U2K71_002855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000342537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEM7316856.1"
                     /translation="MDDMQVYIANLGKYNEGELVGAWFTFPIDFEEVKEKIGLNDEYE
                     EYAIHDYELPFTVDEYTSISELNRLWEMVSELPEELQSELSALLTHFSSIEELSEHQE
                     DIIIHSDCDDMSDVARYYIEETGALGEVPASLQNYIDYESYGRDLDLSGTFISTNHGI
                     FEIVY"
     gene            898..1290
                     /locus_tag="U2K71_002856"
     CDS             898..1290
                     /locus_tag="U2K71_002856"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM7316857.1"
                     /translation="MKKIRSYTSIWSVEKVLYSINDFRLPFPITFTQMTWFVVSLFAV
                     MILGNLPPLSMIEGAFLKYFGIPVAFTWFMSTKTFDGKKPYGFLKSVIAYALRRKLTY
                     AGKKVTLGRNQPQEAITAVRSEFYGISN"
     gene            1274..>2778
                     /locus_tag="U2K71_002857"
     CDS             1274..>2778
                     /locus_tag="U2K71_002857"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:NP_464631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM7316858.1"
                     /translation="MAYPIKYIENNLVWNKDGECYAYYELVPYNYSFLSPEQKIQVHD
                     SFRQLIAQNRDGKIHALQISTESSILSAQERSKNEVTGKLKTVAYDKIDQQTDALISM
                     IGENQVDYRFFIGFKLLLNDQEFSMKSLTVEAKNAFTDFVYDVNHKLMGDFVSMSNDE
                     ILRFQKMEKLLENKISRRFKIRRLDKDDFGYLIEHLYGQTGTAYEEYEYPLSKKKLEH
                     ETLIKTYDLIKPTRCLVEEKQRYLKIQQEDETAYVAYFTINSIVGELDFPSSEIFYYQ
                     QQQFTFPIDTSMNVEIVANRKALSTVRNKKKELKDLDNHAWQSDNETSSNVAEALESV
                     NELETNLDQSKESMYKLSYVVRVSANDLDELKRRCNEVKDFYDDLSVKLVRPFGDMLG
                     LHEEFLPASKRYMNDYIQYVTSDFLAGLGFGATQMLGENEGIYVGYSLDTGRNVYLKP
                     ALASQGVKGSVTNALASAFVGSLGGGKSFANNLIVYYAVLYGAQAVIVDPKA"
BASE COUNT          839 a          518 c          596 g          825 t
ORIGIN      
        1 actggtttct atcaaacgct caatcactgg tgcttttagc aatcgttgtg attggcttgt
       61 atcttggctt caagcgtgag tttagcaaac tgattggctt tttaattatt gcgattattg
      121 cggttggctt agtcttcaac gctgctggag taaaagacat tttactagag ctattcaatc
      181 gcattattgg tgcttaaata aaaccgttct tttgtggaat ataagtggtt ttcttatgtt
      241 ccgcaaagga atggtacacc aaacgaagtg cggtagggat ttttgaatct ctacaaagaa
      301 aggacgtgaa tatatggacg atatgcaagt ctatattgcg aatttaggca aatacaatga
      361 gggcgaattg gtcggtgcgt ggtttacctt tcccattgac tttgaggaag tcaaagagaa
      421 aatcggcttg aatgatgaat atgaggaata cgccattcat gattacgagt taccctttac
      481 ggttgacgaa tacacttcta ttagcgaact caatcgccta tgggaaatgg tatcggagtt
      541 gccagaagaa ctacaatcgg agctatctgc tctgctcact catttttcaa gtattgaaga
      601 actaagcgaa catcaagaag atattatcat tcattccgat tgtgatgata tgtctgacgt
      661 ggcacgctac tacattgaag aaacgggtgc tttaggcgaa gtgccagcca gtcttcaaaa
      721 ctatattgat tatgaatcct atggtcggga tttagacctt tcgggaacgt ttatctcaac
      781 caatcacggg atttttgaaa tcgtctatta aatctgtcgg tacattctta ctggcagatt
      841 ttcttgttta cggggtagtc aaaagactat ccccattttt atgaaaggat tgattacatg
      901 aagaaaatac gaagctatac cagtatctgg tctgttgaga aagtgttgta ctccatcaat
      961 gactttagac ttccgtttcc cataaccttt acgcaaatga catggtttgt cgtgtcactc
     1021 tttgcagtta tgatacttgg caacttgccc cctctttcta tgatagaggg agcatttctc
     1081 aaatactttg ggattcctgt ggctttcaca tggtttatgt ctacaaaaac ctttgatggt
     1141 aaaaagcctt atggattttt gaagtctgtc attgcttatg cactgcgacg aaagctgacc
     1201 tatgcaggaa aaaaagtaac gcttggcaga aaccagccac aagaagccat tacagcagtt
     1261 aggagtgaat tttatggcat atccaattaa atatattgaa aacaatctgg tctggaataa
     1321 agatggggaa tgttacgctt actatgagct tgttccctac aattactcat ttctaagtcc
     1381 ggaacagaaa atacaagtac atgattcttt cagacagctt atcgcacaaa atcgtgatgg
     1441 caagattcat gctttacaaa tcagtacaga atccagcata ctttctgcac aagaacgttc
     1501 caaaaatgaa gtcactggaa agctcaaaac ggttgcctat gacaaaatcg accaacagac
     1561 agacgcttta atatccatga ttggcgaaaa tcaagtggac taccgtttct ttatcggttt
     1621 taagttgctt ctcaacgatc aggagttttc tatgaaaagt cttaccgttg aagcaaaaaa
     1681 tgcttttact gattttgtct atgatgtgaa ccataagctg atgggcgatt ttgttagtat
     1741 gagtaatgat gaaatcctgc gttttcagaa gatggaaaag cttttagaaa ataaaatctc
     1801 ccgtcgtttc aaaatccgaa gattagataa ggatgacttc ggctatctga ttgaacacct
     1861 ttacggacag acaggcactg cctatgaaga gtatgagtat cctctatcaa agaaaaagct
     1921 ggaacatgaa acactgatta aaacctatga cctcattaag cctactcgct gtctggtgga
     1981 agaaaaacag cgatatttga aaatccagca ggaagacgaa accgcctatg tagcttactt
     2041 taccatcaac agtattgtcg gcgaactgga cttcccgtcc tctgaaatct tctactacca
     2101 gcaacagcaa tttacctttc cgattgatac gtcaatgaat gtggaaattg tagcgaatcg
     2161 taaagcccta tctactgtcc gcaataaaaa gaaagaactg aaagacttgg ataaccacgc
     2221 ttggcaaagt gataatgaaa ccagctctaa tgtggcggaa gctctggaaa gtgtgaacga
     2281 gctggaaacc aatttagacc aaagcaagga atctatgtat aagctgtcct atgttgtaag
     2341 ggtatcagca aatgaccttg acgaacttaa acgtcgttgt aatgaagtga aagatttcta
     2401 tgatgatttg agcgtgaaac tggtacgacc ttttggggat atgctgggct tacatgaaga
     2461 atttttacct gccagcaaaa gatatatgaa tgactatatt caatacgtga cctctgattt
     2521 cctcgctggt ttaggttttg gtgctactca aatgctgggt gaaaatgagg ggatttatgt
     2581 tggctacagc ttagatactg gacgcaatgt ctatctgaaa cctgctcttg ccagtcaagg
     2641 ggttaagggt tcagtaacca atgcgttagc gtctgccttt gtcggttcgc tgggtggtgg
     2701 taaatccttt gcgaataacc ttatcgtcta ttatgcagtg ctttatgggg cacaagcagt
     2761 gattgtagac ccaaaagc
//
LOCUS       DASHHB010000141         2640 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.152, whole genome shotgun sequence.
ACCESSION   DASHHB010000141 DASHHB010000000
VERSION     DASHHB010000141.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2640)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2640)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2640
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.152"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            724..2550
                     /gene="ltrA"
                     /locus_tag="U2K71_002858"
     CDS             724..2550
                     /gene="ltrA"
                     /locus_tag="U2K71_002858"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM7316859.1"
                     /translation="MLKKNKLRYNEYYDMQHIYDELYAQSKNGNNFYKLLEIIGSEQN
                     ICLAYRNLKSNSGSKTAGTDGMTIDDIKQLSNAEIVATVRESLSNYRPKSVRRVFIPK
                     AGSDKMRPLGIPCIWDRLVQQCILQVLEPICEPKFHNHSYGFRANRSAHHAVSRVTTL
                     INLSKYHYCVDVDIKGFFDNVNHGKLLKQIWTLGIRDKRLICIISKMLKAEIDGEGVP
                     EKGTPQGGLLSPLLSLIVLNELDWWVSSQWETFQPKNRSKNGWLQYAKKYTKLKSGFI
                     VRYADDFKIMCSTYGEAQRFYHSTVDFLNKRLKLEISPEKSKVVNLKKNSSDFLGFKI
                     KVIPKGRTKHGYVAKTDMNQKALKKAKTNLKLKVKDIARHTTGFNISRYNLTVIGMQN
                     YYCIATNVYNNLTEVSYALLPTIRIRLRNIAKSVPFESTSSEFQSRTKGIRPKTKIVM
                     IADNPLLPIQGVQHKNPMNFSQDICNFTKQGRNKVHEDVVVVTKEEIRALLENENPAD
                     SVEFNDNRISAYIAQQGNCYVMNRRGTPSTLICIHKSDKGDNLDRYSNLAFVEIPIAK
                     AILTESADEAKSLLKGYVLNSQQKKKLNRIRTNYGYQTITGK"
BASE COUNT          948 a          461 c          584 g          647 t
ORIGIN      
        1 aaaagagtag gcacagcact tcattaaatc aatatttaat gtgtgttgga gaactaaaca
       61 cacgaatagt cgaatgacgg ggtaacgccc tgaaaggcta ctttaatact ctgactggca
      121 gaaacttgag taaacaaggc tctgtcagaa gctcggtgaa gtcggcagac agacaccgta
      181 acagataatg ctgacgaaag attcccagag gtgggcgttg tgtcctatat gccggaagtc
      241 tataaaatat ctatggttag aatgtagatg agaggtctac tgacgaatcc ttgaatgtac
      301 aggtctatat cggggctgga tagaaatgtc cagtgacaca aacagtgtcg ctactgtggg
      361 tgagtaagac tccgcattta tgaaaatcca tataacgtta aaggcgttat caaggtagca
      421 ggcttataga ggaaacctaa ggatatatgt acagatagtg tgtttggaac gttgaaagct
      481 gacaacatgg agattttact gtctgtgaag tgttcacatc agaaagttat tgtgagatta
      541 gcaatgatat aaggtgtgtt aatgtcagtg agagtggtgg cacagtacct atgaaaccgt
      601 gataacaagc ggtggaggga tagccactag tcatatgaag aatagaatta ctaagaaaaa
      661 tggtatgggt tcgagtaaga ctaagagatg tacttgctcc tgcaaacaag gagggtacag
      721 accatgctaa agaaaaacaa gctccgctat aacgagtatt atgatatgca acatatttat
      781 gacgagttat acgcacagag caagaacggt aataactttt ataagttact tgaaattatt
      841 ggttcggaac aaaatatttg tttagcttac cgtaatctga aatcaaacag tggcagtaaa
      901 acggctggaa cggatggaat gacgatagac gatataaaac aactcagcaa tgctgagatt
      961 gttgctaccg ttcgggaaag ccttagcaac taccgcccca aatcagtcag acgtgttttc
     1021 attccaaaag ctgggagcga taaaatgcgt ccattgggga ttccgtgtat ctgggacagg
     1081 ctggtacaac aatgtatcct gcaagtccta gagccgatat gcgaaccaaa gttccataat
     1141 cattcctatg gattccgtgc gaatcgaagt gcccaccatg cagtcagcag agtaacgaca
     1201 ctgataaatt tatcaaaata tcattactgt gttgacgtag acattaaagg atttttcgat
     1261 aatgtcaatc atggaaaact attaaaacaa atctggacac ttggtattcg ggataaaaga
     1321 ttgatttgca ttatcagcaa aatgctgaaa gccgaaattg acggcgaggg tgtcccagaa
     1381 aaaggaacgc cacaaggcgg tctgttaagt ccactgctct ccctcatagt ccttaatgaa
     1441 ttagactggt gggtaagcag tcagtgggaa acattccagc cgaagaaccg cagtaaaaat
     1501 gggtggcttc aatacgcaaa gaaatatacc aaactgaaaa gcggttttat tgtacgttac
     1561 gctgatgatt ttaaaatcat gtgttcaacc tatggagaag cacaaagatt ttaccacagt
     1621 accgtagatt ttcttaataa aaggctgaaa ttagaaatca gtccagagaa atcaaaggta
     1681 gtaaatctga agaaaaattc atcagatttt ctggggttca aaatcaaagt cattccaaaa
     1741 ggaagaacga aacacggtta tgttgccaaa acagatatga accaaaaagc attaaagaaa
     1801 gcgaaaacaa atctaaagtt aaaggtaaaa gatatagcca gacatacgac tggcttcaat
     1861 atcagcagat ataatctgac agttatcggt atgcagaatt actactgcat agcaactaac
     1921 gtatataaca acctgacaga agtaagttac gctctcttac cgaccatacg aatccgttta
     1981 aggaacattg caaaatcagt tccatttgaa agtacaagtt cagaatttca gagcaggaca
     2041 aaaggaatca gaccaaaaac aaaaatagtg atgattgcgg ataatccgct attgccgatt
     2101 caaggagtac aacacaaaaa tcctatgaat ttctcgcaag atatttgtaa ttttacaaag
     2161 caagggagaa acaaggtgca tgaggacgtt gtagttgtaa caaaagaaga aatccgagcg
     2221 ttgttggaaa atgaaaaccc tgccgatagc gtagagttta acgacaatcg tatttcggca
     2281 tatatcgcac aacaaggcaa ctgctatgta atgaatcgtc gaggaactcc gtccactctg
     2341 atatgtattc acaaatcaga caaaggagat aaccttgaca gatattctaa tctggcattt
     2401 gtggaaattc ccattgcaaa ggcaatccta acagaatcag cagacgaagc aaaatcactg
     2461 ttaaaaggtt atgttcttaa tagtcagcag aagaaaaaac tgaacagaat acggacgaat
     2521 tacggatacc agacaataac tgggaaataa cttataaatc tatttggata tgatggaacg
     2581 ccgtatgaag cgaaagtttc atgtacggtg tgaagcgggg gaaaacttgg agataacatc
//
LOCUS       DASHHB010000142         2634 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.153, whole genome shotgun sequence.
ACCESSION   DASHHB010000142 DASHHB010000000
VERSION     DASHHB010000142.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.153"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            5..1150
                     /locus_tag="U2K71_002859"
     CDS             5..1150
                     /locus_tag="U2K71_002859"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HEM7316860.1"
                     /translation="MHIQTKLIHGGISEDPTTGAVSVPIYQTSTYRQDGVGQPKQYEY
                     SRSGNPTRFALEELIADLEGGVRGFAFSSGLSGIHAVFSLFQAGDHILLGDDVYGGTF
                     RLFDKVLTKNGLEYTIIDTSNLDKIEQSIKPNTKALYLETPSNPLLKITDLEKSATLA
                     HQHGLIVIADNTFATPYFQRPLDLGSDIVVHSGTKYLGGHSDVVAGLVTSNHKDLADQ
                     IGFYQNAIGAVLGPQDSWLLQRGIKTLSVRMEEHQKNAFVVADFLFSHPAVEKVYYPG
                     LPDHELHGVAKQQMSGFSGMISFTLKNEESAIPFVESLQLFTLAESLGGVESLVEIPS
                     VMTHASIPKEKREEAGIKDGLIRLSVGIEYGQDLINDLAQAFDRIKN"
     gene            1321..1779
                     /locus_tag="U2K71_002860"
     CDS             1321..1779
                     /locus_tag="U2K71_002860"
                     /EC_number="4.4.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005879048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S-ribosylhomocysteine lyase"
                     /protein_id="HEM7316861.1"
                     /translation="MRMNVESFNLDHTKVKAPYVRVADRKTGAHGDVIIKYDVRFKQP
                     NKEHMDMPSLHSLEHLTAELIRNHADYIVDWSPMGCQTGFYLTVLNHESYEDILDVLE
                     ATMKDVLEATEVPASNETQCGWAASHTLEGAQALAREFLAKRDEWSEVEA"
     gene            1938..2408
                     /locus_tag="U2K71_002861"
     CDS             1938..2408
                     /locus_tag="U2K71_002861"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HEM7316862.1"
                     /translation="MSIYNFETTLEDGTVYSLKKYAGKPIIIVNTATKCGLAPQFKQL
                     EELYQAYQTQGLVILGFPSNQFKQELDSATEAAEACKTTYGVTFPMHQLIDVNGENTD
                     PLFTYLKNEAPGTVTKAIKWNFTKFLIDRNGKVIERFAPQTSPLKMTAAIESVL"
     gene            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="U2K71_002862"
     rRNA            complement(2461..2576)
                     /gene="rrf"
                     /locus_tag="U2K71_002862"
                     /product="5S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00001"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00001"
BASE COUNT          863 a          503 c          496 g          772 t
ORIGIN      
        1 aaacatgcac attcaaacaa aattgatcca cggaggcatc agtgaagatc ccacaacagg
       61 agctgttagt gtacctattt atcaaacatc cacttatcgg caagatggcg tcggccagcc
      121 taaacaatat gaatattcaa gatctggtaa tcctactcgt tttgcattgg aagaactgat
      181 tgctgatttg gaaggcggtg ttcgaggatt cgctttttct tctggtcttt ctgggattca
      241 tgccgttttt tctctttttc aagctggcga tcatatctta ttaggagatg atgtctatgg
      301 aggaacattc cgtctatttg acaaagttct tactaaaaat ggtctcgagt acacgatcat
      361 agatacgagt aatcttgata aaatagaaca atccatcaaa cccaatacaa aagcacttta
      421 tcttgaaaca cctagcaatc cgcttctaaa gattactgat ttagaaaaaa gtgccactct
      481 ggctcatcaa catggattga tagttattgc tgacaataca tttgctactc cttattttca
      541 aagaccttta gatctcggtt ctgatatcgt tgtccatagc ggtaccaaat atctaggcgg
      601 acacagcgat gtagtagctg gtttagtgac ttctaaccat aaagatctag ccgatcagat
      661 cggattttac caaaatgcta tcggagccgt acttggtccc caagatagct ggttacttca
      721 gcgcggaatc aagacactaa gtgttcgtat ggaggaacat caaaaaaatg cttttgtggt
      781 agctgatttt ctattttctc atccagcagt agaaaaggtc tactatccag gattacccga
      841 tcatgaactc catggagttg ctaagcagca gatgagcggc tttagcggaa tgatttcttt
      901 tactttaaaa aatgaagaat ctgctattcc atttgttgaa tcactccaac tatttacttt
      961 agcagaaagt ttaggtggcg tagaaagttt agtggaaatt ccttctgtca tgactcatgc
     1021 gtctattcca aaagaaaaac gagaagaggc tggcataaag gacggcttga tccgtctttc
     1081 tgtaggaatt gagtatggtc aggatctcat aaacgattta gctcaggcat ttgacagaat
     1141 caagaactag aagtcccaat aaacgactta gcagaaaaat ctaaaaaata tttcagattg
     1201 ctacaccttg gaaacgataa gtgttagaat caagtagaaa gacgtttttg taaatttcac
     1261 taaataattc tttagtgaaa agcatcaaaa cgaatgaaca aacagatagg agtcttacaa
     1321 atgagaatga atgtcgaaag ttttaatctt gaccatacaa aagtcaaagc accttatgta
     1381 cgtgtagctg atcgaaaaac gggtgcgcat ggcgatgtca tcattaaata cgacgtccgc
     1441 tttaaacaac caaataaaga acacatggac atgccaagtc tacattcact tgagcacttg
     1501 actgccgaac taatccgcaa tcacgcagat tacatcgtag actggtctcc aatgggatgc
     1561 caaacaggct tttacttgac tgtattgaat catgaaagct acgaagacat tttagatgtt
     1621 cttgaagcaa caatgaaaga tgttcttgaa gctactgaag tacctgcaag caatgaaaca
     1681 caatgtggct gggctgccag ccatacacta gaaggtgcac aagctttggc tcgtgaattt
     1741 ttagcaaaac gcgatgaatg gtctgaagta gaagcatagt ttctaaaaga acgtaaagat
     1801 gagaagcaat atagctatct tatcttcgta ttttattaaa tatttattgt ctgaaaagag
     1861 ttctaaacgg gaaaacaaat agctagtttt cagtttagcg ctctttttat tatataaaat
     1921 agaaaggaga aaactttatg tctatttata attttgaaac tactttagaa gacggcactg
     1981 tctattcatt gaaaaaatat gcaggcaaac cgataatcat tgttaacact gcaacaaaat
     2041 gcggtctagc acctcaattt aaacaattag aagaacttta ccaagcttat cagacacaag
     2101 gtctggttat cttaggcttt ccttccaatc aatttaaaca agagctagat tcagcgactg
     2161 aagcagcaga agcttgtaaa accacttatg gcgttacctt ccctatgcat caattgattg
     2221 acgtaaatgg agaaaataca gatcctcttt ttacttatct taaaaatgaa gctcctggta
     2281 ctgtcactaa agcaataaaa tggaatttta ctaaattttt gattgataga aatggcaaag
     2341 taatcgaacg atttgcacca caaacttctc cattgaaaat gaccgctgca attgaatccg
     2401 ttctttaact taataaaaat aaaaaaagat gaactatttc tagttcatct ttttgccttt
     2461 gcgtggcgac gtcctactct cacaaggggc aacccctcac tacaatcggc gctaagaagc
     2521 ttaacttctg tgttcggcat ggttacaggt gtatccttct cgctatcgcc accacactgt
     2581 ggtgttatct tttattgagt aaattttgtt cactcaaaac tggatttgaa gtca
//
LOCUS       DASHHB010000143         2626 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.154, whole genome shotgun sequence.
ACCESSION   DASHHB010000143 DASHHB010000000
VERSION     DASHHB010000143.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2626)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2626)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2626
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.154"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..750
                     /locus_tag="U2K71_002863"
     CDS             <1..750
                     /locus_tag="U2K71_002863"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM7316863.1"
                     /translation="KSNCGRRPLVLPEEQSEYIQRKVVQGWTPDVIVGRAAFPISCSA
                     RTIYRMFKKGLFDSSDLPMKGKRKPNGHQERRGKQTFRRSIHEREKDYSQFSNEFGHL
                     EGDTIVGLKHKSAVITLVERLSKVIITLKPCGRQAIDIEKKLNQWFESVPKNLFKSIT
                     FDCGKEFSNWKQISNVNDIAIYFADPGTPSQRGLNENSNGLLRRDGLLKSMDFNSVDE
                     FFIQSVASKRNNIPRKSLDYRTIIEHLWKYF"
     gene            2301..>2626
                     /locus_tag="U2K71_002864"
     CDS             2301..>2626
                     /locus_tag="U2K71_002864"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7316864.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKERPVS
                     CGMRTL"
BASE COUNT          957 a          362 c          526 g          781 t
ORIGIN      
        1 aaatcaaact gtggtagacg tccgcttgtt ttacccgagg aacaatcaga atatattcaa
       61 agaaaggttg ttcaaggatg gacacccgat gtgattgttg gtcgtgcagc gtttcctatt
      121 agttgttctg ctcgtaccat ttatcgtatg ttcaaaaagg ggctatttga ttcttctgac
      181 ttaccgatga aaggcaagcg taaaccgaat ggacatcaag aaagacgtgg aaaacaaact
      241 ttccgccgct ctattcatga acgtgaaaag gattatagcc aattctcaaa tgagtttggt
      301 caccttgaag gtgacactat cgtaggtctg aaacataaaa gtgctgtaat taccttagtt
      361 gaacgattat caaaagttat catcacattg aaaccgtgtg gtagacaagc gattgatatt
      421 gaaaaaaaat taaatcaatg gtttgaatct gtaccgaaaa acctattcaa atccatcact
      481 tttgattgtg gaaaggaatt ttcaaattgg aaacagatca gtaatgtcaa tgatattgcc
      541 atttatttcg ctgatccagg aacgccgtct caaagaggcc taaacgagaa ttctaacgga
      601 ttgttacgta gagatggttt attgaaatct atggatttca attcagtaga tgaatttttt
      661 attcaatctg tcgcatctaa acgaaataat attcctagaa aatcactgga ttatcgaacg
      721 attatcgaac acctttggaa gtatttttga gttacgtaag tattgatgat ctgtctaatt
      781 taatttgaca attaagaaag tattaacttg ttttgtgaaa aagagaattt gaaaaacgat
      841 tggcgaacaa atgctcggta ggtatggtat aaatacagag ctaattggtg tgatgaaatc
      901 agatattcgc gtgagattca gtaaagtagg agttcgaaaa cagctaggtg acaaacagta
      961 ttgctttggt acttcggaat ggtttatcaa attcttatca acagttcact tctctttatg
     1021 tttgagtgat gattcgttag gttattgctt ggaaagaccg gatgagagta gaagagttta
     1081 attttataaa gtgaaaagag gtaatgtacg atatacgtag ggctgaacca atagaggata
     1141 agcaaacgat gttacaggcg caaaattcat agcttactgt tttggagata acagaagagg
     1201 aaataatgca gaagtaaggc gaaaaaagga cggaagccta acagcatatg aaataaagaa
     1261 aaatattata actactgata aatcggtatt gagcaaagta ctaataggag tcaaaaacat
     1321 tcataagcta tgagtgtttc ttggctactt ttttattttt atgtatctac aaaagtttcc
     1381 aggggcagaa cctgtgatta ttaatggaag aacctcaagt attgtcttaa atcattgtta
     1441 tgaaaatgga ttaaaagagt tcatcaaggt gtgattaaca agttactttt cattacatcg
     1501 aatttttcag aagttgctta tattggtgat gatattaatg atttatcatg tatgacatct
     1561 gtgaagaagc gggaggaata gtagaatgtc caaaaaatgc cacaagaaat gagattgaat
     1621 tatctgattt tattgtaact cacagtggtg gtgacgaatc tgtgatagag tttattgagt
     1681 ggatattgga atcgatatag tgtagtagta cagaaaaatt atcatacaat taatactagg
     1741 cataagaata ttggcatatt ctagagttaa aagtaactat tcgaaaagga taaggttaga
     1801 attttttgtt ttgccagtgg aagaatataa aaagggcatt ggaaattaat atattagaat
     1861 gctaagcgga gaaaaaaata tatgaaaagt acgtccttgt gatgggatgg aaaaatacac
     1921 ttatttcaaa caacgataat ataattatgt gaaccgaagt atactcgctt ctaattcaat
     1981 cctcaaatcc cgacagtcgc gacttcaaaa aagcgtatag ttttaatgta tccccatatg
     2041 atccgaaaaa tggtaattta caattaacag tttgatgatg ttgaagatgt ggaaatgctg
     2101 aaattaccat gatgagaaac acattaatta aagggaatta attaactaga atataatctg
     2161 attcaatttg aattaagcaa aaaaaggctg agttttaaat aatcgcgaat tgtaaaatta
     2221 agttagaaaa ataaaaaggc atttatggta cactcaaatt gtatttccga cgaaagaaaa
     2281 caaaggagtg taacccataa atgacctaca aacatcttac catagacgaa ctgacaatga
     2341 tagaatcata ttatcttcaa cataataagc cggttgaaat cgctaaccga atgggacgtg
     2401 ctatacaaac tatttataat gtagtcaata agttcaagca aggcaagact gctcttgatt
     2461 attggcacca gtataaagaa aataagaaaa aatgtggtag aaaagtcatt caattacctg
     2521 ctcatgaagt agattacatt aaagagaaag tcactcttgg ttggacgcct gacgtcatta
     2581 tcgggcgaaa agaaaggcct gtttcatgcg gtatgagaac acttta
//
LOCUS       DASHHB010000144         2603 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.155, whole genome shotgun sequence.
ACCESSION   DASHHB010000144 DASHHB010000000
VERSION     DASHHB010000144.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.155"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            16..177
                     /locus_tag="U2K71_002865"
     CDS             16..177
                     /locus_tag="U2K71_002865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002311671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316865.1"
                     /translation="MAIVVIGLFLGFKREFSKLIGFLVIALIAVGLVFNASGVKDVLL
                     SLFNRIVGA"
     gene            190..693
                     /locus_tag="U2K71_002866"
     CDS             190..693
                     /locus_tag="U2K71_002866"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010718210.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antirestriction protein ArdA"
                     /protein_id="HEM7316866.1"
                     /translation="MEQMRVYIANLGKYNEGELVGAWFTPPVDFDEVKERIGLNDEYE
                     EYAIHDYELPFEIDEYTPIEEINRLCGLAEELEGTPVGEVASEIQHAFFNSFEEMVEH
                     VDDIIYYPDCDDMSDVAYFLIVEAGDFGEVPEHLKTYIDFDACGRDLEIGGNYLVTSR
                     GVFEYPY"
     gene            753..1142
                     /locus_tag="U2K71_002867"
     CDS             753..1142
                     /locus_tag="U2K71_002867"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM7316867.1"
                     /translation="MKKIKSYTSIWAVEKVIYAINDFQLPFPVTFNQMAWFVLSLLFV
                     IVFAHVPPLSMIEGAFLKYLGIPVAVTWFMSQKTFDGKKPLGFLRSFISYHLRFKVTF
                     AGKKVKEQKKRWDEPITLVRSVNYVPD"
     gene            1129..>2603
                     /locus_tag="U2K71_002868"
     CDS             1129..>2603
                     /locus_tag="U2K71_002868"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM7316868.1"
                     /translation="MYPIKYIENNLVFNQEGECFAYYELIPYNYSFLSPEQKYQVHDN
                     FRQLIAQNREGKIHALQIATESSIRATQERSKKEITGRLTEVAKQRIDLQTEALVSMI
                     GDSQIDYRFFIGFKLIATDEEVNLKNLKKSFFSGFQEFVYGVNHHLMGDFVSLSNEEI
                     RRYTKLEKLMESKLARRFKVRRVTPSDLMYLIEHIYGEKGTPFEEYEFQLPKKKLKSE
                     TLVKRYDLLRPSRCLIEEKPRYLCMEHENHESYVAYLTINTIVGEMEFPSSELFYYQQ
                     QQFTFPIDTSMNVEIVTNKKALATVRNKKKELKDLDNHAYQSDNETNSNVLDALDSVD
                     ELETTLDQSKESMYKLSYVVRVSAESVDELKRRCDEVLDFYDDTNVKLVRPFGDMMGL
                     HEEFLPSSKRYMNDYIQYVTSDFLAGLGFGATQMLGELEGIYFGYNVDTGRNVYLKPA
                     LASQGVKDSVTNALAAAFLGSLGGGKSFSNNLLVYYAVLFGG"
BASE COUNT          774 a          430 c          599 g          800 t
ORIGIN      
        1 caatccttag tcttactggc aattgtggtc attggtttgt ttttaggatt taagcgggag
       61 ttttccaaac tgattggctt tttggtgatt gctttgattg cggtcggatt ggtctttaat
      121 gcttctgggg tgaaggatgt cttactcagt ttgtttaacc gaattgtcgg tgcgtaaggg
      181 ggaagcacga tggaacagat gcgtgtttat attgcgaacc tcgggaaata taacgaaggt
      241 gaattggtgg gggcgtggtt taccccacct gtcgattttg atgaagtgaa agaacgaatt
      301 gggttaaatg acgaatacga ggaatatgca attcatgatt atgaacttcc ctttgagatt
      361 gatgagtata cgccaattga ggaaattaat cggttgtgtg gcttagcgga ggaacttgaa
      421 ggaacgccag ttggtgaagt tgcttctgag attcaacatg ctttcttcaa ttcttttgag
      481 gagatggtag agcatgtgga tgatattatc tattatccag attgcgatga tatgagtgat
      541 gtagcatatt tcttgattgt agaagctggt gattttggag aagtaccaga acacttgaag
      601 acgtatattg attttgatgc gtgtggtaga gacttggaga ttggaggcaa ttatttggta
      661 acgagtcgag gtgtttttga gtatccatat taacaaaatt attgaagggt agttgatttc
      721 taaactatcc ttctttttaa aggaatgatt tgatgaagaa aataaaaagc tacaccagta
      781 tttgggcagt tgaaaaggtc atttatgcaa ttaatgactt tcaactgcct tttcctgtga
      841 cttttaacca gatggcttgg tttgttttat ccttgctgtt tgtcatcgta tttgcccatg
      901 tgcctccact atctatgata gagggggcct ttttaaaata cttaggtata ccggtggcag
      961 ttacttggtt catgtcgcaa aaaacgtttg acggtaagaa accattggga tttttacgat
     1021 cttttattag ctatcactta cgatttaagg tcactttcgc aggaaagaaa gtcaaagaac
     1081 agaagaaacg atgggacgaa cccattactc tggtaaggag tgtgaactat gtacccgatt
     1141 aagtatattg aaaataatct tgtctttaat caagagggag aatgttttgc ctactatgag
     1201 ttaatacctt acaattactc ctttttgtca cctgaacaga aatatcaagt ccacgataac
     1261 tttcgtcaat tgattgcgca aaatcgtgaa ggcaagattc atgccttaca aattgccaca
     1321 gaaagtagta ttcgtgccac gcaagaacga tcaaaaaagg aaattaccgg gcgattaaca
     1381 gaagtagcga aacaacgaat tgatttacag acagaggcat tagtatccat gattggcgat
     1441 agtcagattg attatcgctt ttttattggc ttcaaactga ttgcgacaga tgaagaggtc
     1501 aatctaaaga atctcaagaa atctttcttc tccggctttc aagaatttgt ctatggagtg
     1561 aatcatcatt tgatgggcga ttttgtttcc ttatccaatg aagaaattcg ccgatatacc
     1621 aagcttgaaa aactaatgga aagtaaattg gctcgaagat ttaaagtcag acgagtcaca
     1681 ccgagtgatc tgatgtattt gattgaacat atctatggcg aaaagggtac accatttgaa
     1741 gaatatgaat ttcagctacc aaagaaaaag ctgaagtcgg aaactttggt gaaacgttac
     1801 gacttattac gtcccagtcg ttgtttgatt gaagaaaagc cccggtactt atgcatggaa
     1861 catgagaatc atgaatcata tgtagcgtac ttaacaatta atacaattgt gggggaaatg
     1921 gagtttccct cctcagaact tttctattac cagcaacaac aatttacatt cccaattgac
     1981 actagtatga atgtggagat tgtgacgaac aaaaaggcac ttgctaccgt tcgcaataag
     2041 aaaaaggaat taaaagactt ggacaaccat gcctatcaat ctgacaatga aacaaattcc
     2101 aatgtcttag atgcattgga ttccgtagat gaattggaaa ctaccttgga tcagtccaaa
     2161 gaatccatgt acaaactgag ctatgtggtt cgagtaagtg ccgaatcagt agatgaacta
     2221 aagcgccggt gtgatgaagt gctagatttt tatgatgata ccaatgtaaa actagtccgg
     2281 ccttttggcg atatgatggg gctacatgaa gaattcttac cttctagtaa gcgatacatg
     2341 aatgattata tccagtatgt tacgtctgat tttctcgccg gacttggttt tggcgccact
     2401 cagatgttag gggagttgga aggcatttac tttggttata acgtagatac ggggcgcaat
     2461 gtttacctga aaccggcatt ggcctcacaa ggggtcaagg attcagttac caatgcgcta
     2521 gcagcggcat tcctcggttc actcggtggc ggaaagtcct ttagtaacaa tctcttagtt
     2581 tattacgccg tcctttttgg tgg
//
LOCUS       DASHHB010000145         2492 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.156, whole genome shotgun sequence.
ACCESSION   DASHHB010000145 DASHHB010000000
VERSION     DASHHB010000145.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2492)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2492)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2492
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.156"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(367..1659)
                     /locus_tag="U2K71_002869"
     CDS             complement(367..1659)
                     /locus_tag="U2K71_002869"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEM7316869.1"
                     /translation="MARKKRNPDAEKLAESILNAYQPESVDDMQDALKDVFGPLFEKM
                     LQGELNNHLGYDAHSKEPKEHDNRRNGYGTKTLKTSFGEVAIDVPRDREASFEPELIP
                     KRKRDVSDIEGKVLSMYARGMSQRDIAATVEAIYGFDISHEMISDITDAVLPELEEWQ
                     ARPLAKCYAFLFVDCMYVTLRENYEAKEYAVYTILGYDLKGNKEILGLWLNQTESKNR
                     WMQVFDELKARGVEDVFFISMDGVSGLEEGAKAIFPSVIVQRCIVHLVRNALRYIPSK
                     DYKEVCRDMKKFYGASSLNAAHAAFDSFQNRWSHYSGAVDVWKRNFAHVEQLFDYGSA
                     IRKIMYTTNAVESVHSSFRKVTKKGAFSNENALLKLLYLRTKELHAKWSGGRIQNWAM
                     VLNQLMINETFSSRMKSMRFTFRNNGRFIQLCVRSKFI"
     gene            complement(1933..2193)
                     /locus_tag="U2K71_002870"
     CDS             complement(1933..2193)
                     /locus_tag="U2K71_002870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316870.1"
                     /translation="MKKIDLINMIGMLIGILVNIVIFTDWLGVLFSNLIPILIIGICG
                     IILSILELFESRNTMNRIFACIILIVNLLPMVYFTFLYFALG"
BASE COUNT          844 a          465 c          382 g          801 t
ORIGIN      
        1 tgtatgggct actctgtgat acttattagc cattaaacta ttttacccac atagcatata
       61 ttttgaaatc gttcattccg taaattcaac taaatatgcc taaaaaaata aaaaatctaa
      121 gatagaataa gatttattca gtcttagatg gatttatagc aactttttcg taacaacttt
      181 aaaaaatacg aaaagaaaga gctgttttac tctgactacg ttgctaaatt gtcgtttaat
      241 cattcattta aaccaaatct ttttttatag aattcgtttt aatcctcctg aatccaaaaa
      301 ctatgaaaac cgtaattaaa tagttaagag tttttaacca ccataattct ctcggctatt
      361 ttgaaatcag ataaattttg atcggacaca tagttgaata aacctaccgt tgttacggaa
      421 ggtaaatcgc atacttttca ttctagagga gaaggtctcg ttgatcatta actgattaag
      481 caccatagcc cagttttgaa ttcgaccacc tgaccacttc gcgtgtaatt ctttcgtgcg
      541 taaataaagt agttttagga gtgcattctc attagagaac gctccttttt ttgtgacttt
      601 tctgaagctg gagtggacac tttcaaccgc attggtagtg tacataattt ttcgaatggc
      661 actaccataa tcaaatagtt gttcaacatg tgcaaagttc cgtttccaga catctacagc
      721 accagaataa tgagaccacc gattttgaaa actgtcaaaa gcagcatgtg cagcgtttag
      781 agaagaagca ccgtagaact ttttcatatc tcggcagact tccttatagt ccttacttgg
      841 aatatagcgc aatgcatttc gaacaagatg aacaatacag cgctgaacaa ttaccgatgg
      901 aaagatcgct tttgcgcctt cttcgaggcc agaaacaccg tccatcgaaa tgaaaaagac
      961 atcttcgaca ccacgtgctt tcagttcatc aaatacttgc atccagcgat ttttagattc
     1021 tgtttgattt aaccataatc ctaaaatctc cttatttcct ttgagatcat agccaagaat
     1081 ggtgtataca gcatattctt tggcttcata attttctcgt aaagtaacat acatacaatc
     1141 aacgaataga aaggcataac actttgctag ggggcgggct tgccattctt ccaattcagg
     1201 aaggacagcg tcagtgatat ctgaaatcat ttcatgggaa atatcaaagc cataaatagc
     1261 ttcgacggtt gcggcaatat ctcgttgact cattcctcgt gcatacatgg aaagaacctt
     1321 cccttcgatg tcggagacat ctcgttttct cttaggaatt aactctggtt caaaggaagc
     1381 ttcccggtct ctaggaacat caatagctac ttcaccaaaa ctggttttaa gcgttttagt
     1441 tccatagcca tttcgacggt tatcgtgttc cttaggctct ttagaatggg catcataacc
     1501 taaatgatta ttcaattctc cttgaagcat tttttcaaaa aggggcccaa acacatcttt
     1561 caaagcatct tgcatgtcat cgacagattc aggttgatag gcattcagaa tggattcagc
     1621 taacttttcg gcatcaggat ttcttttctt tctagccatc gtgtaatcac ctcttgatct
     1681 tattgtagaa aaagaaaaac cgcgaagcaa ccacctgcct aggattaatg gttacttaca
     1741 cggtttatat tacactctca tcattaaaaa agtatctttc gatattacta aacaatgaaa
     1801 ttatctattt gaaatttaaa tctgataata tattgagtgt tccaatgagg gctttgatcc
     1861 tattatccat atctttttca gttttaatat tgatacacat tataagaaaa agaaactttt
     1921 ggttaattta tttcatccca gtgcaaaata taaaaaagta aaatagacca taggcaatag
     1981 attaacaatt aatataatac aagcaaatat tctattcatt gtatttctgc tttcaaaaag
     2041 ttctaatatt gaaagtataa ttccacaaat gcctataata agtatgggaa taagattaga
     2101 aaataacacc ccgagccaat ctgtgaaaat tactatattt actaatatcc ctattaacat
     2161 acctatcata tttattaaat caattttttt cattagatat ttctccttta ttaaatctga
     2221 agtacttata ttaaaataat tctaaactat ttacctcaaa aagtgcaata aattatttct
     2281 gaacaataaa atgatagtca aaacaaaaaa atattgttaa tcgttcagag ccatccgaga
     2341 gtgtaaaata ttttgtgtaa atgaaaaaat ccatacaaaa aaggaaggcg cttctgtaga
     2401 ataaagttaa cgacaaccaa ttcacagaaa agaggacttc cctatgaatg attttactac
     2461 agaaattgtg caaactctag tcactaaagg cg
//
LOCUS       DASHHB010000146         2398 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.157, whole genome shotgun sequence.
ACCESSION   DASHHB010000146 DASHHB010000000
VERSION     DASHHB010000146.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2398)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2398)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2398
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.157"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            207..2069
                     /locus_tag="U2K71_002871"
     CDS             207..2069
                     /locus_tag="U2K71_002871"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM7316871.1"
                     /translation="MQQLTKRERQLLIYLVEMSEPVTIKKLAEKENVSVRTIKYDLDD
                     IREWLNERKQVLYSKRSQGIWMNISSSDRIKLKSELMDVNRFEIFADQSLRIKRLLIM
                     LLVTKEAITGMKMANALDVSKDTIMNDLDVLENNFLKEGLTLNRQARKGFWIIGEERL
                     VRLTIEEILQKEFTDYDIYKLMSLLLNGGETEYFEMYSATATPIQEVFNQVIIRMRHL
                     LEFENLEKLNYAELLNILIRVTIATVRLRKEATIGRYQLVAEQELDKNDLSYRVMQQV
                     FDYYQLPLFEDEYHYIYSDTFDTIHQQDVMKLVENVIYKVSKAINHSFYQDPQLLTNL
                     YAHLSMRLSRKKKFVNEYNPFKEDIKQKYPELFDAIKQAVRQEISEQNILINDSFVAY
                     IALHFLVSYEREEDLRSVRAVYICSTGLGVTSLIKQKINEELNNITIVAFASVLNAQE
                     MIATKDPDLVISIFPINGIDRPFIKVHPLPTEQDLKKIQQIIKKILASSNQQERHNLN
                     LRETSINKDTLEDFSKELILKAYTIYERLLVILEAELIQEYREAFLLHVFLMVHRITF
                     DQQYMAEGRQVDQIILDKGTLVGKIEAVFAENNLGINQSEISALFSYIKEGASS"
     gene            2069..>2398
                     /locus_tag="U2K71_002872"
     CDS             2069..>2398
                     /locus_tag="U2K71_002872"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM7316872.1"
                     /translation="MKMSDEALKIIEESQNREKLKQVIDLIEERLVEHAIVPTELQWT
                     ILINHLNEMLKRSQRKETIPVVDRDLFKEVSKEAITISDEIVRSIGNLTDDEIYVLSI
                     HFETAKNN"
BASE COUNT          865 a          353 c          466 g          714 t
ORIGIN      
        1 aaaaatttga aaaagcgttt gattatttat attacgactt agtgcaataa aaaacgcttt
       61 ttaacagtac atgtagtcgt ctttataata gataaactct gtaatattag ttaaaaagct
      121 ataagttgca cttagttaag tgcaaacatt tcatattgtc gttagtttag aaaacgctta
      181 tattagcaat gtaaggagag atgataatgc agcagctaac aaaaagagaa aggcagcttt
      241 tgatttactt ggtggaaatg tctgaaccgg taacaatcaa aaagttggct gaaaaagaaa
      301 atgttagtgt tcggacgata aaatacgatc tagatgatat aagagagtgg ctgaatgagc
      361 ggaagcaagt cctatatagc aaacgtagtc agggaatttg gatgaatatt agtagctctg
      421 atcgaatcaa attaaaaagc gaattgatgg atgttaatcg ttttgaaata tttgcagatc
      481 aaagtctccg aatcaaacgt ttactaatta tgcttttagt aacaaaagaa gctattactg
      541 gaatgaaaat ggccaacgca ttagatgtca gcaaagatac gatcatgaat gatttagatg
      601 tgctggaaaa taattttctt aaggaaggat tgaccttgaa tcgtcaggcg cgaaagggtt
      661 tttggatcat aggagaagaa cgtctagttc gattaacgat tgaagagatt cttcaaaaag
      721 agttcacgga ttatgacatt tataaattaa tgtcattgtt attgaatggc ggtgagactg
      781 aatactttga aatgtattct gctactgcta cacctatcca agaggtattt aatcaagtaa
      841 tcattcgaat gcgacactta ctggaatttg aaaatctaga gaaactgaat tacgcggaat
      901 tactaaatat tctgattcga gtgacaattg ctaccgttcg cttacgcaaa gaggctacga
      961 ttggtcgtta tcaattggtt gctgaacaag aattagacaa aaacgatctt tcttatcgcg
     1021 tcatgcagca ggtctttgat tattatcagt tgccactgtt cgaggatgaa tatcattata
     1081 tctatagtga tacattcgac acgattcacc agcaagacgt catgaaacta gttgagaatg
     1141 tgatttataa agtaagtaaa gcaatcaacc attcttttta tcaagatcca caattgttga
     1201 cgaatctgta tgctcattta tccatgcgat tatcgagaaa gaaaaaattt gtcaatgaat
     1261 ataatccttt taaagaagat atcaagcaga aatacccgga attatttgat gcgatcaagc
     1321 aagcagttcg acaagaaata agtgaacaaa atattctgat caatgattct ttcgtggcat
     1381 atattgcctt acactttttg gtttcctacg aaagagaaga agatctacga agcgttagag
     1441 ccgtctacat ttgttcaaca gggttgggtg tgactagtct gatcaagcaa aagatcaatg
     1501 aagaattaaa taatatcact atcgtggcat ttgcttcagt attgaatgcg caagaaatga
     1561 ttgctaccaa agatcctgat ttggtgatta gtatttttcc aattaatggc atcgatcgtc
     1621 ctttcatcaa agtccatcca ttacccactg aacaggactt gaaaaaaatt caacaaataa
     1681 taaaaaaaat acttgcttct tccaatcagc aggaaaggca taacttgaat cttagagaaa
     1741 cgtccatcaa taaagatact ttagaagact ttagcaagga attgattttg aaagcttata
     1801 cgatttatga acgtttactt gtcattttgg aagcagaatt gatccaagaa tatcgagaag
     1861 cttttttgct tcatgtattc ttaatggttc atcgtatcac atttgatcaa caatatatgg
     1921 cagaaggtcg tcaagtagac cagattattt tagacaaagg aacactggta ggaaaaatcg
     1981 aagcagtttt tgcagaaaat aatttaggaa tcaatcagtc agaaatcagt gcattattct
     2041 catatataaa agaaggagcg tcaagttagt gaaaatgtca gacgaggctt taaaaattat
     2101 cgaggaaagt caaaatcggg aaaagttaaa acaagtgatt gatctaattg aagagcgatt
     2161 agtagaacat gctattgtac caaccgagtt gcagtggacc attctaatta atcacttgaa
     2221 tgagatgctg aaacgttctc aaagaaagga aacgattccc gtagtcgatc gggatttgtt
     2281 caaagaagta tccaaagaag ccataactat ttctgatgaa atagttagaa gtatcggtaa
     2341 tttaaccgat gatgaaatat atgttttgtc gattcatttt gaaacagcaa aaaataat
//
LOCUS       DASHHB010000147         2272 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.158, whole genome shotgun sequence.
ACCESSION   DASHHB010000147 DASHHB010000000
VERSION     DASHHB010000147.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.158"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(716..2239)
                     /locus_tag="U2K71_002873"
     CDS             complement(716..2239)
                     /locus_tag="U2K71_002873"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEM7316873.1"
                     /translation="MDPTVFEADEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKEPDWLTKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSVQQEEMQKIIEEYNQYYDTSWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVKSSTLNNQIQLLEEQIGVYETVKGSLIEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDDLIHVFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKANHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          658 a          450 c          354 g          810 t
ORIGIN      
        1 actaatacag gtagtcctct tttttgttat tttgatattc taaatcattt tttattggat
       61 tattcccttg ttcgatatag acccaaattg atctatcaat aattatcaaa ctgagtcata
      121 tagagacaaa aatttcaaaa atgacctgtt aaatggtcat cgaatatcat ttaaaatcaa
      181 tacgacggag tcagacacgg agtcaaaaca agcccgctga gagtaaaaaa tatatacagt
      241 cccataggta aagtgccaca ggactgtata tttttttatt tgagattacc tgaaatgatt
      301 ccatttactt tttatctcat tttttcctac tagttagtat caaatactct cctattttct
      361 caataaacta ttttgggtta catggtatct gaaacagcta aaaccttgtc attacagcaa
      421 aaaatcactt tatctccgtt tcatttcaga tacatttcac ttaagcggat tccaaaaaaa
      481 caaaagaaaa aggaacccct agttagccta tgctttttat tggttcctct caaaacacgg
      541 tcatataaca acggggtggg gtaaatgaat gaaaaaggtg gaggaaacat tattcaatag
      601 ctgtcttaaa tacttcgtta taaaatgtga acaaataaaa gacatctatc cctattttct
      661 acacttccac tttactacca tctttgaatc gtacaatttt cttaactctt ctcgtttacc
      721 tcaattcatt ttcaagtgga atcagctcat catctaacat tttacgccat gctcgcttca
      781 tttcttgtgg gtatttaaat ggcttcgctt ctggatggtt ggctttatat ccttcgtaat
      841 ccttactgtt gataattgct ttcatattag gaattggatc ttcacctggc atatattgca
      901 gattagaagc atgtaattct ttctcagaaa caaaccactc attggaaaag acatgaatca
      961 aatcatcact cgcttgtgta aaatagcgac gtttaatgac aaagatatct tcattactat
     1021 ctaaagtccc cacatcaaac gcatttaaaa tttgtcggta aacttctttg actccttctg
     1081 ttttattcaa tttagccaaa gctttttcaa tttcttctcg tgcatctgaa ctattcgccg
     1141 cataagttcc tagtaattga tcaatataag tagaatcaat atcatacaac ttcgtggtat
     1201 tttcactata aaattcgatc gtggaaaaat cttctccctc tttttctggt tcttcctcta
     1261 ttaatgaacc cttcacggtc tcgtacacgc caatttgttc ttccaaaagt tgaatttgat
     1321 tgttcaaggt tgaagatttc accatgtcat cattgtaatc gtcataagtc actaacgatt
     1381 cataagcacg attcaattct tggaatgctt tgacgtactc gatacggatt tcaagtggcg
     1441 tatgttcatc aatggattcc atggatgaaa cctcttttaa cttaccatat gcttgcttga
     1501 atcttttttt ggattcctga taacttggat aaatcaaacc cgattcttct tttgcttctg
     1561 aatacagttt tgtcgcatcc tcgacattct tctccattgt ggctggcttt cggaaagtaa
     1621 caattagtcc ttttgttttt tctggatacg tacggttcgt acgtgaaaaa gcttggatca
     1681 atcctgcata ctctagatta cgatccacaa atagggtttg aatcgtaggt gcgtcaaaac
     1741 ctgtcaatag acgatccact acgatcacta aatcaatttg tttcccaaat tgtttgaatt
     1801 ccgctctttt tcgtgccaaa cgattattga tgtctccgtt ataacgctca atgtctgcta
     1861 atgaccagga agtatcataa tactgattat attcctcaat aattttttgc atttcttctt
     1921 gttggacact tgaatctcgg ctgttttctt ctaatgaata ggtaatagcg attcgtggaa
     1981 aatcaggatc ctccatcgtt cttccttcac gaatgggctg attaggaaac tcttttgtta
     2041 accagtcagg ttctttcgtc atttctttga tcgctcgata atactttttg gccatgtcaa
     2101 tggaactcgt ggttaagatc gcagacttcg ttggacggcc atttcgaaaa tcaaattttg
     2161 tataggcatt gtctggacgg aacattttat gaatgacttt ttggatatgt tcatctgctt
     2221 caaaaacagt agggtccaaa taactttctt ttttgacagg ctccatttga tc
//
LOCUS       DASHHB010000148         2179 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.159, whole genome shotgun sequence.
ACCESSION   DASHHB010000148 DASHHB010000000
VERSION     DASHHB010000148.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2179)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2179)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2179
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.159"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(121..591)
                     /locus_tag="U2K71_002874"
     CDS             complement(121..591)
                     /locus_tag="U2K71_002874"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316874.1"
                     /translation="MSIISFILSTFLTGIKIKGMRPQLEVELNASYFAADMIYTKVII
                     SNYSTEPAMLVNLELSSANLNRRWSATPFKKLIAKGGSVDDNRIYSEAVPLNIPPKSA
                     ISCYLAFEVGKINFNKLLNHQTKMIFTLNRTQIHKIVDIKNTNFPIEKLVKELN"
     gene            678..1376
                     /locus_tag="U2K71_002875"
     CDS             678..1376
                     /locus_tag="U2K71_002875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016177536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage regulatory protein"
                     /protein_id="HEM7316875.1"
                     /translation="MQELVILKNKEAVTTSLQVAESFEKKHQHVLRDIDALKKDVSNF
                     GQMFVEGNEPDSYGRNRRVFFISRDGFFLLAMGFTGKKAIYFKQKYIEAFNEMEDVIR
                     KNTVPQTIEDMMIYQLEEMKDVKKDVSMLKDTMRISGQQEFEIKQKGNMKVMGVLGGK
                     ESRAYEEISKKVFSKFWSEFKRTFSIPRYGELPRKRFDDAVSFIEMWLPETAIRMEID
                     QLNRQQRLFGDDNE"
     gene            1554..1895
                     /locus_tag="U2K71_002876"
     CDS             1554..1895
                     /locus_tag="U2K71_002876"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002353384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316876.1"
                     /translation="MEKELSTLDQYLIDPDWGKPKIEETSGRKIRRNLLTNEELAWDQ
                     DDLGNHVTIWDHVYLIHLSKHSNKPEYIYVIEDGLIDALEEYDRDNLIDISYYGPGKK
                     YIAEMEAEFDE"
     gene            1888..>2179
                     /locus_tag="U2K71_002877"
     CDS             1888..>2179
                     /locus_tag="U2K71_002877"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEM7316877.1"
                     /translation="MSEIKGTTNFEKLFSRKLNKILKKKGNFDYLSWAHAWEIMKKND
                     PQATVTINEYKHYRVVSGTHQDFLVEEYKPFLMDETGTYVSVSVTVKGHTETE"
BASE COUNT          798 a          311 c          437 g          633 t
ORIGIN      
        1 atggtttgga tttttatggg gcttgatgaa gctgaagctg agtacagagc gaaaaagcca
       61 accgaggggg ctgactaaaa atgaatagaa aagagaaact agaatgtata ttactattac
      121 tcagtttaat tctttcacta atttctctat tgggaagttt gtatttttga tatcaacaat
      181 tttgtggatt tgggttctat ttagagtaaa tatcattttt gtttggtgat taaggagttt
      241 attaaaatta attttcccta cttcaaatgc aaggtaacaa gatatagcac ttttaggagg
      301 aatatttagc ggtacagctt cagagtatat tctattatca tcgactgatc ctcctttagc
      361 aattaatttc ttaaatggag tagctgacca tctgcggttt aagttagcgg atgaaagttc
      421 taaattaact aacatagctg gttctgttga atagttagaa atgattactt ttgtataaat
      481 catatctgca gcgaagtaag aagcattaag ttcaacttct agttgagggc gcattccttt
      541 tatttttatc cctgttaaaa acgtactcaa tataaaactt attattgaca tccattgaaa
      601 aattgttaag tgaaaaaaat tcaaaataaa caccaccagt ttttaactaa attataccaa
      661 aaaggagaga agaaataatg caagaattag taattttgaa aaataaagaa gctgtgacta
      721 cgagcttaca agtggcagaa agcttcgaga aaaaacatca acatgtttta agagatattg
      781 atgcactaaa aaaagatgtg tccaattttg gacagatgtt tgtagaaggt aatgaaccag
      841 attcatatgg cagaaatcga cgagttttct tcattagtag agatggattt ttcttgctgg
      901 ctatggggtt tacaggaaag aaagctatct acttcaaaca aaaatacatt gaagcattca
      961 acgaaatgga agatgttatt cgcaagaata ctgttcctca aacaattgaa gatatgatga
     1021 tctatcaatt agaagaaatg aaagatgtta aaaaagatgt ttccatgctt aaagatacta
     1081 tgcgaattag cggacaacaa gagtttgaaa ttaagcaaaa aggaaatatg aaagttatgg
     1141 gagttctagg gggaaaagaa agccgagctt atgaagaaat cagcaaaaaa gtattctcaa
     1201 aattttggtc tgaatttaaa cgtacctttt caatcccaag atatggcgag ttacctcgta
     1261 agagattcga tgatgctgtt tcatttattg aaatgtggtt gccagaaact gcgatccgta
     1321 tggaaattga tcaactgaac agacaacaga gactttttgg tgatgacaat gaatagagct
     1381 gaagcgctaa gaatagggac ggcaattgct aattgctggt ggaaatacta taaaccaatc
     1441 atcctaagcc aacaacatat tgacaagcaa aaagcatggc aacaaataaa aaagtgactc
     1501 cgccggcaag caaagagtca caaagaaaac acatcataag gagattttag catatggaaa
     1561 aagaactttc cactctagat caatatttga ttgatcctga ttggggcaag ccgaaaattg
     1621 aggaaacaag tggtcgaaaa atcagacgaa atcttttgac gaatgaagaa ctagcttggg
     1681 atcaagatga tttaggcaac catgtaacta tttgggatca tgtttatctt atccatctat
     1741 cgaagcattc gaataaacct gaatatattt acgtcatcga agatggcttg attgatgcgc
     1801 tagaagagta cgacagagat aacttgattg atatctctta ttacggacca ggtaagaaat
     1861 acattgctga aatggaggca gaatttgatg agtgaaatca aagggacaac gaactttgaa
     1921 aaacttttta gtcgtaagtt aaataaaatt ctcaagaaaa aaggaaattt tgattattta
     1981 tcttgggctc acgcgtggga gattatgaaa aagaatgatc cacaggcaac ggtaactatt
     2041 aatgagtaca aacactacag ggttgtttct ggaactcatc aagactttct tgttgaggaa
     2101 tataaacctt ttcttatgga tgaaactggg acttatgtat ctgtctcagt aacggttaaa
     2161 ggacacacgg aaaccgaat
//
LOCUS       DASHHB010000149         2133 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.160, whole genome shotgun sequence.
ACCESSION   DASHHB010000149 DASHHB010000000
VERSION     DASHHB010000149.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.160"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            24..248
                     /locus_tag="U2K71_002878"
     CDS             24..248
                     /locus_tag="U2K71_002878"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin"
                     /protein_id="HEM7316878.1"
                     /translation="MNNKTFEVLKWFALVIIPALATFVGLVGKALNWQYTDICVVIIT
                     GFGAFLGSVLGVSNRTYKMFSAESEEGGNK"
     gene            245..1270
                     /locus_tag="U2K71_002879"
     CDS             245..1270
                     /locus_tag="U2K71_002879"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002324905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HEM7316879.1"
                     /translation="MKKKITITAMSLLTALFLLPINTFAYTINDEYNLAPNQGDSRLA
                     IPNKIILHETGIDAPARNVAANMKNNYNGSNSYTTDVIGDGGIVYRVGEQGYVSWGAG
                     NANPYAPVQIELQRTYDKALFEKNYRAYIEYTRDSAKKYGIPLTLDQGTSLFTKGIIS
                     HLWVTNYVWGNHTDPYGYLSQMGVSKEKLAYDLAHGFTDENPTTSENKPVIDPTRAGA
                     ANPTLTDGTNHAHIDQFGEIENANLHVAGWHIANYKYEYIFIMDYNTGKELARVRADG
                     IYRPDVNQAYNTSGNVGYHVSFNMRNFPSKKVYVMMRATNDPEGNTKGGAQDFHDKRW
                     YLNIPKR"
     gene            1460..1533
                     /locus_tag="U2K71_002880"
     tRNA            1460..1533
                     /locus_tag="U2K71_002880"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1494..1496,aa:Ile,seq:cat)
BASE COUNT          698 a          361 c          417 g          657 t
ORIGIN      
        1 actttattta gggagagaag aaaatgaata ataaaacgtt cgaagtacta aaatggttcg
       61 cactggtaat tattcccgca ctagctactt tcgtggggtt agttggtaaa gcgctcaatt
      121 ggcagtacac agatatctgt gttgtcatca ttactggttt tggcgcgttt ttagggagtg
      181 tgttgggtgt atcaaatcga acctacaaaa tgttctcggc tgaaagcgaa gaaggaggaa
      241 acaaatgaaa aagaaaatta ctattactgc gatgagcctg ttaacggctc tttttttatt
      301 acccattaat acgtttgctt atactattaa tgacgagtat aatttagcgc cgaatcaagg
      361 agactccaga ttagcaattc ctaacaaaat tattttgcac gaaactggaa tagatgcacc
      421 agcaagaaac gtagccgcca acatgaaaaa taattataac ggaagcaatt cttatactac
      481 agatgttatt ggtgacggtg ggattgttta ccgtgtgggt gagcaaggat atgtttcgtg
      541 gggagctggt aacgctaatc cttatgcgcc tgtacagatt gaattacagc gcacatatga
      601 taaagcattg tttgaaaaaa actatcgagc ttatattgaa tatacaagag atagtgcaaa
      661 aaaatatgga attccattga ctcttgatca aggaacttct ttatttacaa aaggaatcat
      721 ttctcatttg tgggtgacaa attatgtttg ggggaaccac acagatccat atggttactt
      781 atcgcaaatg ggagttagca aagaaaagct tgcttatgat ttagctcatg gatttaccga
      841 tgaaaatcca acaacttctg aaaacaagcc tgtcattgat ccaacacgag ctggtgcagc
      901 taatcctacg ctgacagatg gaacaaatca cgcccacatt gatcagtttg gggaaatcga
      961 aaacgcaaac ttgcatgtgg ctggatggca cattgctaac tataaatacg agtatatttt
     1021 cattatggac tacaatactg ggaaagaatt agctcgagta agagctgatg gaatttatag
     1081 accagatgta aaccaagctt ataatacttc tggaaatgtt ggttatcatg tatctttcaa
     1141 tatgcgtaat tttcctagta agaaagtcta tgtaatgatg cgggcaacga atgatccaga
     1201 gggaaacact aaaggcggtg cgcaagattt ccatgacaaa cgttggtatt taaatattcc
     1261 taaacgataa aaatagctcc tcgttgagga gcagtacata actatattga caactataaa
     1321 aattattcga taaaatagtg atgttatcgc atatcttcac tatcacccat aaatagtcac
     1381 actccaagct atgcgataac aggtttgttg ccacacattc tactggttga ttgtttatgg
     1441 ctttatgtgg caacaaccag tacccttagc tcagttggtc agagcagacg gctcataacc
     1501 gtccggtcgt aggttcgagt cctacagggt acattaacgt agccatttga atcgttctgt
     1561 gttagaattt ttttgaagag tattatacaa gctaaagctt ttcttcattg ccactcaaat
     1621 gagtggcttt tttatgtatc cttttatgga ttaatgaaag gatgtttcac atagttatac
     1681 ttctgtatat ttgaaaagtt ttactttgat ttttaaatag aaagacattt gggttaaatt
     1741 gtgagataat aataaagaag agtttaaagc gcaccccaaa ccacttcccc ataagtgtgt
     1801 tacgctttaa actcttttat atttgaagcc attaaaaagc ataccatatt tttgaaaaaa
     1861 agtgagaaaa aaggcttaca attggagtgg tagttaatta gtgacttatt tttgatttta
     1921 tagcactgat actataaaat atagatatca tcatattaca caatcttaat actaacttaa
     1981 aaaatatctc ctttcacaag tatggtgata aaattcgttc cgggctacct ttttaggtag
     2041 cctactttaa tctttatacc tttctggatc aacgaaagta tactttatat agtcataacg
     2101 ccgatgatcg ctacgtgcgt ccggcacgtc agt
//
LOCUS       DASHHB010000150         2023 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.161, whole genome shotgun sequence.
ACCESSION   DASHHB010000150 DASHHB010000000
VERSION     DASHHB010000150.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2023)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2023)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2023
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.161"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..993
                     /locus_tag="U2K71_002881"
     CDS             <1..993
                     /locus_tag="U2K71_002881"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316880.1"
                     /translation="DGPYWLGKVPLGENIADHTVKFPIESGDHQIYQGYTEEELMIGQ
                     TYTITLKGTKPASQTFVAYNHWTARLGELKPVDGLTDVWSLTFTPTNVVASSPKLFRV
                     YQYPQSTVGACRIDWLKIEKGDTRTPNISEYKYRGTGMRDSNNPKDYVWDLAPEYVED
                     NLATDIKISEITGKANNYTDGKVSEINSQLTASINEVDTTAKDAQTKANANATAIDEL
                     DNKIDERINDTATTTLTVTNGNTGSAKLYREGKTVSIYFVALNGKSSGGNDSTILTIP
                     EGYRPPISFEQLVGSIDRSTLNSAQLSIGADGAIKWRRNSSYGSDYTFAITYTI"
     gene            1010..1159
                     /locus_tag="U2K71_002882"
     CDS             1010..1159
                     /locus_tag="U2K71_002882"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002290627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316881.1"
                     /translation="MKAAYRPIEPYGFEQIIVNDEEHLPEECTEVEPPIPNWKPKFNY
                     WEGNK"
     gene            1156..1602
                     /locus_tag="U2K71_002883"
     CDS             1156..1602
                     /locus_tag="U2K71_002883"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316882.1"
                     /translation="MKNIWKYGRTGGEYAGKVLDDMLVSVPYTDQPPLEGIRADGEPL
                     TIADQMFDPKLNQWIILANALDHNDLNNLKAMYESLENENGDLKQINAKLMLSDVAIK
                     QENTALKEKADSLAQINSKMMLASLQNSKDISEIKEQLNPASKGGE"
     gene            1604..1741
                     /locus_tag="U2K71_002884"
     CDS             1604..1741
                     /locus_tag="U2K71_002884"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XkdX family protein"
                     /protein_id="HEM7316883.1"
                     /translation="MFSFSDVKMMYDWGCFTNEQVMVFVPLCITEEKADKIISKEESA
                     S"
     gene            1779..>2023
                     /locus_tag="U2K71_002885"
     CDS             1779..>2023
                     /locus_tag="U2K71_002885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002341747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316884.1"
                     /translation="MINLGEWGAIAGSITAIVSLILLVIKPITASFSKITETLSKVSH
                     NLDLLTKDLESSKSDRLMIHEELKKHDERLDTHAEKLV"
BASE COUNT          717 a          347 c          425 g          534 t
ORIGIN      
        1 gatggccctt actggttagg taaagttcct ttaggcgaga atattgccga tcatacagtt
       61 aagttcccaa tcgaatctgg cgaccaccaa atatatcaag gttacacaga agaagagctt
      121 atgataggtc aaacgtatac tatcacgctt aaaggaacaa aacccgcaag tcaaaccttt
      181 gtagcgtata atcattggac tgctcgttta ggagaactaa agccagttga tgggttgaca
      241 gacgtatggt ctctaacatt cacaccaacg aatgttgtgg cgagttcacc taaacttttt
      301 cgtgtttatc agtatccaca atcaacagta ggcgcatgcc ggatcgactg gctcaagatt
      361 gaaaaaggcg acacacgaac cccgaatatt agtgagtata aatatcgtgg tactggtatg
      421 cgtgattcaa acaatccaaa agattatgtt tgggatctag caccagaata tgtcgaagat
      481 aacttggcca cagatattaa aatttctgaa attactggta aagcaaacaa ttataccgat
      541 gggaaagtat cggagattaa ttcgcagttg actgcttcaa ttaatgaagt agacaccaca
      601 gctaaggatg ctcaaacaaa agcgaatgct aatgcgactg ctatagatga attagacaat
      661 aagatcgatg aacgcattaa tgatacagct actaccacat taacagttac aaacgggaat
      721 accggatcag caaagcttta tcgtgaagga aaaacagttt ctatatattt tgtggcttta
      781 aacggaaaaa gcagtggtgg aaatgattca acgatactaa caattccaga aggctatcgg
      841 ccaccaatta gttttgagca actggttggc tcgatagacc gttctacttt gaacagtgct
      901 cagttatcta ttggtgcaga tggagccatt aaatggcgaa gaaactcaag ttatggatcg
      961 gattatacct ttgcaattac ttacacgatt tagaaaagcg tgaatcgata tgaaggcagc
     1021 atatagacca attgaacctt acggattcga gcaaatcatt gtgaatgatg aagaacattt
     1081 accggaagaa tgcacagaag tcgaaccacc gattccaaat tggaaaccga aattcaatta
     1141 ctgggaggga aataaatgaa aaacatttgg aaatatggac gtactggcgg agagtacgca
     1201 ggaaaagtat tggacgacat gcttgtatcc gttccttaca cagatcagcc accgcttgaa
     1261 ggaattcgtg ctgatggcga accgttaaca atcgctgatc aaatgtttga tcctaaattg
     1321 aatcaatgga ttattttagc gaacgcacta gatcacaacg atttaaacaa tctcaaagca
     1381 atgtatgagt cgttagaaaa tgagaacggc gatttaaaac agatcaatgc caaactcatg
     1441 ctaagcgatg tagcaattaa acaggaaaat actgcattga aagaaaaagc ggatagttta
     1501 gcacaaatca attcaaaaat gatgcttgct tcgttacaaa atagcaaaga catttcagaa
     1561 attaaagagc aactaaatcc agcttcaaag ggaggtgagt agtatgttta gttttagcga
     1621 tgtgaaaatg atgtatgatt ggggctgttt cactaacgaa caagtaatgg ttttcgttcc
     1681 gttgtgcatt actgaagaaa aagcagataa aatcattagc aaagaagaga gcgcatctta
     1741 attgatgtgc tttttatttt gattcaagga gttgtcacat gattaattta ggggaatggg
     1801 gagcgatagc aggatcaata accgctatcg tttctttgat tttattagta ataaaaccaa
     1861 ttactgcatc tttctcgaag attactgaga ctctttcaaa agtaagtcac aatttagatt
     1921 tgctgactaa agatttagaa tcgagcaaat cagatcgatt gatgattcat gaagaactaa
     1981 agaaacacga tgaaagatta gatacacatg cagaaaaatt ggt
//
LOCUS       DASHHB010000151         2014 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.162, whole genome shotgun sequence.
ACCESSION   DASHHB010000151 DASHHB010000000
VERSION     DASHHB010000151.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2014)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2014)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2014
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.162"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(95..193)
                     /locus_tag="U2K71_002886"
                     /pseudo
     CDS             complement(95..193)
                     /locus_tag="U2K71_002886"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS30 family transposase"
     gene            complement(458..913)
                     /gene="tnpA"
                     /locus_tag="U2K71_002887"
     CDS             complement(458..913)
                     /gene="tnpA"
                     /locus_tag="U2K71_002887"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605 family transposase"
                     /protein_id="HEM7316885.1"
                     /translation="MSNDDKSLAYTRWNCKYHIIFTPKYQRKVIYGQLRKDLGKILRK
                     LCEMKDVEIIEAHAMLDHIHMLVRVPPKICISSFMGYLKGRSAVLIHEQHANLKYKYG
                     NKSFWSKRYYVSTVGLNQKTNEKYIREQEADDRIRYIISKREYVEPFKK"
     gene            1156..2014
                     /locus_tag="U2K71_002888"
                     /pseudo
     CDS             1156..2014
                     /locus_tag="U2K71_002888"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611166.1"
                     /note="frameshifted; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
BASE COUNT          638 a          385 c          368 g          623 t
ORIGIN      
        1 atctgtattt aatacagatt ttatttatta gttcatctgc ctattgtatt ttattttgtt
       61 gccttaccat ctgaagtaaa tatactctta attgtcaaat taagttagac agatcatcaa
      121 tacttacgta actcaaaaat acttccaaag gtgttcgata atccagtgat tttctaggaa
      181 gattatttct gcgccagcat tttctaatgt ttgaatttgt gttttgtaat cttggttgct
      241 tgtgctaatt cttgaatagc cgtaaattgc caaaatttca tcaccctttc tggacataag
      301 tttctgacac cagttaaaat caagtctatc agtaacccat acacccgtga ggacgggtgt
      361 atttttaatc cccctagaag gggtcgacac tcgctgcccc ttaaaagggg atttttaaaa
      421 gaccgccaac tgctcatctt cactcactta cttctcttta ttttttaaat ggttctacgt
      481 actctctttt acttataata tatcgaatgc gatcatcagc ttcttgttca cggatgtatt
      541 tttcatttgt cttttgattc aatccgactg tactcacata gtatctcttc gaccaaaagg
      601 atttgtttcc gtatttatat ttgagatttg cgtgctgctc gtggattagt accgcactac
      661 gacctttcag ataccccata aaactggata tacatatttt tggtggtact cgaacaagca
      721 tgtgaatatg atctagcatt gcatgtgctt ctatgatttc cacatctttc atttcgcaca
      781 acttcctaag tatttttcca agatcttttc gtaattgccc atagattact tttcgttgat
      841 attttggtgt aaaaataata tgatacttac aattccaacg agtatatgct aaacttttat
      901 cgtcatttga catgaaaaaa ctcctttcga tctgatgagc ggttggtagc cactcactag
      961 tatatcgaaa ggagttttta tcgctaaaga tacttcttcc acgcgtagaa acgcgcggtt
     1021 ttcttgtttc gcacgcttta cgcacaaaac tgactgaagt cattaagaaa ataaaagggt
     1081 ctaaaacttt taagttttgt accgaccccc aaaagttaga ccaaaaaatc taacttttgg
     1141 aggtcggtat tttttatggc taagtatact tttgaactta aattaaagat cgttcatgat
     1201 tacttggatg gtaaaggcgg aagtgattat ctggcaaaga aatattccat taaggctcca
     1261 tcacaagtga agcgctggat caatgcctat caagaatttg gtgaagaggg tctagtaaga
     1321 aagcgacaaa agaaaaaata ttctgttcaa tttaagctgg atgcgataga gttatatcta
     1381 acaagtgagc tgtcctatcg tgaagttgcc aacacactta acatgaacaa ccctaatttg
     1441 atcgctagtt ggatgcgtca attccgtgag ggtgggatcg atggactctc aaaaacgaaa
     1501 ggatgtccac ctatcttgtc taagaaaaat gaacccaaaa ataagaaaaa atctacaact
     1561 aaagcaacgt ctaaagaacg tgaacgcatc gaggagttgg agaaacgagt ccgttccctt
     1621 caaattgaaa atgccttttt aaaagaattg aggaagctgc gaaaacagga agcccaacaa
     1681 cgacgaatga agcaatcgca cgaatcatcg caagcctccg aggaccgttt aaattagttg
     1741 agcttctaga aactttaaag ttccctaagg caatctttat gtactggcaa aaacgattgg
     1801 accgtaaaaa tccagatcaa gagattgaag cggaaatgct taaagttcgg gagaagcaca
     1861 aggattatgg ctgtttacgg atgaccaatg aactaagaaa ccgcggtttc tctatcaata
     1921 aaaagaaagt tcagcgcctg atcaagaagc taggaataaa agtaacgaca tacacacgta
     1981 aatcacggcg ctacaattct tatcgaggac aagt
//
LOCUS       DASHHB010000152         2003 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.163, whole genome shotgun sequence.
ACCESSION   DASHHB010000152 DASHHB010000000
VERSION     DASHHB010000152.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.163"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..378
                     /locus_tag="U2K71_002889"
     CDS             <1..378
                     /locus_tag="U2K71_002889"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HEM7316886.1"
                     /translation="KNVSTEDFFNYCRLEESGVTVTSGTDLPLFITDIPESIIRAVFR
                     RFPEGEEVWEKETSFTTTELLKSFTSNSFKVNGLSEYGEIKVGKSASLAVFSEDLCTD
                     DWQKVMAAEVIATYLDGDLVYKK"
     gene            426..1826
                     /gene="yfcC"
                     /locus_tag="U2K71_002890"
     CDS             426..1826
                     /gene="yfcC"
                     /locus_tag="U2K71_002890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative basic amino acid antiporter YfcC"
                     /protein_id="HEM7316887.1"
                     /translation="METMKRKFTFPHSYTLIFILIILAAILTWIIPSGQFETVVENVA
                     GMEKTTVVPGTYHQIDKADYGQGFSSILMAPAEGIISAVEVVAFVLIVGGAFGIILKT
                     GAIDRGLFTLAKVLGDKGILVIPMSMILFSLGGSTFGMSEEVIPLFAVFISLMFSLGY
                     DSMTAILILFLSTQAGYVGSTINPFNVLIAQGVAGVYGNPLLWFRMLSWIVITGISIV
                     FTMLYASRVKKNPLSSIVYESDLKVKHKFQNFDTEKEFSTQDKLIIAGFIVGLGIMIW
                     GIISKGWYMVEISALFLGLGLFAGGIARFSQKEIAEAFVEGCSDFAYAAVIIGLARGV
                     LVILEGGMIIDTILNSLANLLGGLPKFLFTTILLISQVIVSFFVPSSSGAAALTMPVM
                     APLGDLMNVSRDVVVLSNQFGNGLMNIISPTSGVLLAGLAIAEINFSKWFKVGFKIFA
                     ILTLVSAVLLYIATLF"
     gene            1859..>2003
                     /locus_tag="U2K71_002891"
     CDS             1859..>2003
                     /locus_tag="U2K71_002891"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316888.1"
                     /translation="MIKINQLAGMNIHYLFYSLKEFFAYQQKIGISSVELWGGAPHFF
                     IDAF"
BASE COUNT          555 a          339 c          432 g          677 t
ORIGIN      
        1 aaaaatgttt ccacagagga cttctttaat tattgtcgat tagaggaaag tggtgtaaca
       61 gtcactagcg gtacagattt accgctgttt attactgata ttcctgaatc gattataaga
      121 gcagtattcc gtcgttttcc tgaaggagaa gaagtctggg agaaagaaac aagcttcacg
      181 acaaccgagc tgctgaaaag ttttacaagc aacagcttca aagtaaatgg tttatcggag
      241 tacggagaaa taaaagtagg gaaaagcgcc agcttagctg ttttcagcga ggacctctgt
      301 actgatgact ggcaaaaagt gatggctgcg gaagttatcg ctacctatct tgatggtgac
      361 ttagtttaca agaaataatg cttttcgata taacgttata aaacgaatta aagagaggtt
      421 aaaaaatgga aacaatgaaa agaaaattca ccttcccaca tagttataca ttgattttta
      481 tcttaattat tttggctgca attttaactt ggataattcc tagtggtcag tttgagacag
      541 ttgttgaaaa cgtcgcaggt atggaaaaaa caacggttgt tccgggaacc tatcaccaaa
      601 tcgataaggc agattatgga caaggttttt cctctatact aatggcacca gctgaaggga
      661 ttatttcagc tgttgaggtt gttgcttttg ttctcatcgt tggtggtgcc ttcgggatta
      721 tcctaaaaac gggagccatc gatcgagggc tgtttacctt agcaaaagtt ctgggagaca
      781 aggggatctt agttattccg atgtcgatga ttctattcag cttaggagga tcaacctttg
      841 gcatgagcga ggaagtcatt cccttatttg cggtgtttat ttctctgatg ttttctcttg
      901 gctatgattc catgactgcg atcttgattc tatttttaag tacacaagcg ggatatgttg
      961 ggtcgacgat taatcccttc aacgttttaa tcgcacaggg agtagctggc gtttacggaa
     1021 atcccctgct ttggtttcgg atgctatcct ggattgtcat tactgggatt tcaattgttt
     1081 tcaccatgct ttatgctagc agagtcaaaa agaatccact gtcctctatt gtttatgaga
     1141 gtgatttaaa ggttaagcat aagtttcaaa attttgacac tgaaaaagag ttttcaacac
     1201 aagataaact aattatcgcc ggttttatcg taggtttagg aatcatgatt tggggtatca
     1261 tttccaaggg atggtatatg gttgaaattt ctgcactatt cttaggatta ggtctttttg
     1321 ctgggggcat cgctagattt tctcaaaagg aaattgctga agcttttgtc gaaggctgtt
     1381 cagatttcgc ctatgctgct gtaatcattg ggctagctag aggagtcttg gtaatcttgg
     1441 aaggtggaat gattattgac actattctaa acagcttggc taacctttta ggtggtcttc
     1501 caaaattcct gttcactacg attctactca tttctcaagt aattgtttcc ttttttgttc
     1561 catcctcctc aggagctgcg gcattaacga tgccggttat ggcaccattg ggtgatctaa
     1621 tgaatgtaag tcgtgatgtt gttgttctaa gtaaccagtt tggaaatggt ttaatgaata
     1681 ttatctcccc aactagtggt gtattactgg caggattagc catcgcagag attaatttct
     1741 ctaaatggtt taaggttggc ttcaaaatat ttgccatctt aacccttgtt tctgctgttt
     1801 tgttatacat tgctacactc ttttagtctt aatcatccat taataggagg caatagttat
     1861 gattaaaata aatcaattag caggaatgaa tatccactat ttattttatt ctctcaaaga
     1921 atttttcgct tatcaacaga aaattggtat ttcttctgtt gaactttggg gaggtgctcc
     1981 gcattttttc atagatgctt tta
//
LOCUS       DASHHB010000153         1847 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.164, whole genome shotgun sequence.
ACCESSION   DASHHB010000153 DASHHB010000000
VERSION     DASHHB010000153.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1847)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1847)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1847
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.164"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            700..>1847
                     /gene="ltrA"
                     /locus_tag="U2K71_002892"
     CDS             700..>1847
                     /gene="ltrA"
                     /locus_tag="U2K71_002892"
                     /EC_number="2.7.7.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group II intron reverse transcriptase/maturase"
                     /protein_id="HEM7316889.1"
                     /translation="MPKNKNETLCVEDLRHAEYYEMQNTFDDLYAKSKNGDIFTHLMD
                     IILSRENILLAYRNIKANAGSKTAGTDGTIIKDIGKLPAETVVKKVRYIVAGSPHGYR
                     PKPVRRKEIPKPNGKTRPLGIPCMWDRLIQQCIKQVLEPICEAKFSENSYGFRPNRSV
                     ENAIKATYNRLQISQLHYVIEFDIKGFFDNVNHSKLIKQIWAMGIRDKHLIFILKRIL
                     KAPIKMTNGTITYPEKGTPQGGIISPLLANIVLNELDHWVESQWQENPVTKNYVVHIN
                     KSGSPCKSNAYKEMKKTKLKEMYMVRYADDFRVFCRYKESAEKAKIAITQWIEQRLKL
                     EVSQEKTRIVNVRKRYSDFLGFKIKMIPRRKKLVVKSHISDKQFKNQK"
BASE COUNT          730 a          281 c          384 g          452 t
ORIGIN      
        1 aaaagctaag gcactaataa aagaacgaaa aagtttggtt attaggagaa ctgataatct
       61 gacaggtgga atgatgaagt aacgctctga aacgcctgcc ctgagcctcc gacaagcatt
      121 gtatgaggaa actcagaatg tttgaagctc ggtaaagacg gctgaaagat accgtaatag
      181 ttgatgaaca tatatcttct atcgaaaact atccagaggt gggtagcgta tccaacaggt
      241 cgggggtcta taaaatatct atggttagaa tgttctataa ggaactgacg aaacagcgaa
      301 tgtacgggtc taaatttgaa ctttgtagaa atgcaaaggt acgagaaatc gtagctggtg
      361 tggcaaagta agaatatagg ttatgaaatg ccatataacg ttacaggcgg tatcaagcca
      421 gcaggcttat agctagtgac ctaaggatat atatgtatag ataagattat cggaacgagg
      481 aaaggtattg ggttctcatc aagagaataa actgacgaag aacaataagc agttgaacta
      541 atgctgaaaa gcagagggcg aacctaagaa attcttgtaa tgagaatgga ggaatgccct
      601 caagtcgtgt atatattaga aaagtattat gttgtttcaa taaggattac gagtaagata
      661 aaaaagctca ctcaatttca agtaaaggag tgaagcctta tgcctaaaaa taaaaatgaa
      721 acattgtgtg tagaagattt aagacacgct gaatattacg aaatgcaaaa cacctttgat
      781 gatttatatg ccaaaagtaa gaatggagat attttcactc atcttatgga tattatccta
      841 tcgagagaaa atatattact ggcatatcga aatatcaaag caaatgcagg cagtaaaaca
      901 gcaggaacag acggtacaat aattaaagat attggcaaat tgccagcaga aacagttgtc
      961 aaaaaagtaa gatacattgt tgcaggcagt ccacatgggt atcgacctaa gccagtgagg
     1021 cgaaaagaaa taccaaaacc aaatggtaaa accagacctc taggtattcc ttgtatgtgg
     1081 gacaggctta tacaacaatg tataaaacaa gtattagaac caatctgcga agctaaattt
     1141 agtgaaaaca gctatggttt tagacctaat aggtcagttg aaaatgcaat aaaagcaacc
     1201 tataaccgcc tacaaatcag ccagctacat tatgtgattg aatttgatat taagggtttc
     1261 tttgacaacg tgaaccattc caagctgata aaacagatat gggctatggg aatacgggac
     1321 aagcacttga tattcatttt aaaaaggata ttaaaagctc caatcaaaat gactaacggt
     1381 acaataacat atccagagaa aggaactccg caagggggaa taatatcccc actactagca
     1441 aacattgttc ttaatgagtt agaccattgg gtagaaagcc aatggcagga aaatcctgtt
     1501 acaaaaaact atgtggttca tatcaataaa agcggtagcc catgtaaaag caacgcttac
     1561 aaagaaatga agaaaacaaa gttaaaggaa atgtatatgg ttcgttatgc agatgatttt
     1621 agagttttct gccgctacaa ggaaagtgct gaaaaagcca aaatagcaat cactcaatgg
     1681 attgaacaac gattaaaatt ggaagtatca caagaaaaaa caagaattgt aaatgttaga
     1741 aaaagatact ccgactttct tggtttcaag attaaaatga taccgagaag aaagaagtta
     1801 gttgttaaat cacatataag tgataaacaa ttcaaaaacc aaaaaga
//
LOCUS       DASHHB010000154         1791 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.166, whole genome shotgun sequence.
ACCESSION   DASHHB010000154 DASHHB010000000
VERSION     DASHHB010000154.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1791)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1791)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1791
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.166"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            1..357
                     /locus_tag="U2K71_002893"
                     /pseudo
     CDS             1..357
                     /locus_tag="U2K71_002893"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
     gene            363..1049
                     /locus_tag="U2K71_002894"
     CDS             363..1049
                     /locus_tag="U2K71_002894"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316890.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGREISLK
                     DESNTTVSDANILANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVTNFKNKYQIKGIKLNQ
                     ETIKKLRIGG"
     gene            1052..1279
                     /locus_tag="U2K71_002895"
     CDS             1052..1279
                     /locus_tag="U2K71_002895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002390667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316891.1"
                     /translation="MINLNAVAFEQTWRTKYKKMSPRDKLFLEIMTFAFIGTQAEQSD
                     ISVEKIKTNRLVNGITETCYQYTIIVVDEEE"
     gene            1297..1620
                     /locus_tag="U2K71_002896"
                     /pseudo
     CDS             1297..1620
                     /locus_tag="U2K71_002896"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016617897.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
BASE COUNT          660 a          293 c          367 g          471 t
ORIGIN      
        1 aaaccaacca gttgttaaac caaatgcaat gcaaatcaat aactcattga agcgatgctt
       61 tgtgaaagca ttggctctac acggactggg attatatgta tttcaagggg aagatattcc
      121 aacaccacct agaatcgata caaagaaatt aaacatgcta gagacgattc tagagctttc
      181 aatgagcaga tgggtaaaga tatgaccaaa accttaatcg aatatgttaa tgagcagaca
      241 gataagttag ggctcttagc tgataacgtt gaaactattg aacagttaag ctatgagcaa
      301 tgtgccttga tggagcgagc aatagcagct aagagaaaag aattagataa gaagtgatat
      361 gagtgtttaa accattaatc gattcatatt cagcggttct gaaaaagttc aaaggaaaag
      421 acataagcgc aaccatcaat gaggaagtga acattgatcg actaaagacg atgtatgacg
      481 gctacgatgg tgatcgagtt attgaaattc gttttattga tcctagacgt ttcacagtac
      541 agcaacgaaa cttcatctat gcactcatag gcgatatttt catcgataca ggcatgccaa
      601 cggacttctg gaaggaattc ttttacttcc gtttcgaagg tgtcacaggg cgcgaaataa
      661 gcctcaaaga tgaatcgaat acgactgtga gtgatgccaa tatcttagca aatatcatct
      721 tagattttat ctttgaacat catattcctt tcaaagaagg ctatgagatt ttaccagcga
      781 atcaagaata ttacttctac aaatgcatca caaaaagagt ctgctgtatc tgtggcaaaa
      841 caggagctga tatcgatcac tttgacaaag cgctaggaag acgaaagcgc aaagaagttg
      901 atcattcaga gtacacattt gcagcactct gtagaattca tcacacggag aaacacaaaa
      961 taggtgtgac caatttcaaa aataagtatc aaatcaaagg gattaagtta aaccaggaga
     1021 caatcaagaa attaagaatc gggggctaat gatgattaat ttaaacgcag tagcgtttga
     1081 acaaacatgg cgcactaagt ataaaaaaat gagtccaaga gacaaattgt tcttagaaat
     1141 catgacattt gctttcattg gtacacaagc tgaacaaagc gatattagtg ttgaaaaaat
     1201 taagacgaat agattagtta atggaattac agagacttgt taccagtaca cgattatcgt
     1261 cgtggatgag gaggaataat tttggcagaa agaagaatgt tcgcaaagac aattattgat
     1321 tctgatgcat tcctagatat gcctttatcg acacaggcat tatatttcca tctatcaatg
     1381 cgcgcagatg atgatggatt tattaataat cccaaaaaaa tacaacgtat ggttggttgt
     1441 ggtgatgatg atttaaaact gcttatggca aaaagattca tcttagtttt cgagagcggt
     1501 gtgatcgtca ttaagcattg gaaaatacac aactatattc gtaatgatcg ttacaaaccc
     1561 acgttatatc aggatgaaaa agctttacta gcagacaaag ataacaaagc ttatacattt
     1621 gcagaagagc tttctaaaca tgatgaaaaa cttggtatac cagatgacaa ccaagcggta
     1681 taccaaatgg atacacaggt taggttaggt aaggataggt taggtaagga tagtaaagag
     1741 ataaaagatt taacgccttc gaaaaaatcg aaggctaagc ccatccgtca t
//
LOCUS       DASHHB010000155         1788 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.167, whole genome shotgun sequence.
ACCESSION   DASHHB010000155 DASHHB010000000
VERSION     DASHHB010000155.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1788)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1788)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1788
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.167"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            15..284
                     /locus_tag="U2K71_002897"
     CDS             15..284
                     /locus_tag="U2K71_002897"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316892.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGINNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLSELEQV"
     gene            289..453
                     /locus_tag="U2K71_002898"
     CDS             289..453
                     /locus_tag="U2K71_002898"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316893.1"
                     /translation="MDLKTFTAQIELMHQEALRQSASYEDKWLNTFHGGRESALDQVL
                     KLLKGERRDG"
     gene            446..763
                     /locus_tag="U2K71_002899"
     CDS             446..763
                     /locus_tag="U2K71_002899"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002332753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316894.1"
                     /translation="MDKKAAMKRIIELTHSESWQEDEEVVAEVQRLGKIANGRIRKKP
                     DKRKQRKRKIVVVLHEGKILMQGTASELSAETGYTRGTIRTYAWRNHTDRKGHEYKYL
                     EEE"
     gene            763..1578
                     /locus_tag="U2K71_002900"
     CDS             763..1578
                     /locus_tag="U2K71_002900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002304472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HEM7316895.1"
                     /translation="MNENKLIKLGVAGAVIVGIGVIGGFKFFEKIDNGYVGVRYSMNG
                     GIKDEALTQGVKFVGIDKVIQYPIRLQTIQSKNISVSTSDGKKTTIDIKYDYKVDSTK
                     AAKMYKEFGNITSEDIESGWLKSKLQKVAREVYAKYSLLDVLSGDSSKVEAEVLTNFA
                     KSVESKGFEVEDVTLGVPDVDKETQKSIDAIIRAGQENEKAKLDAETAKTQADSEAYK
                     KTKAAEAEAESNRKVAESVTDNLIRYEEAQARKKHGWVTVNGADTVVTDEAGK"
BASE COUNT          678 a          277 c          407 g          426 t
ORIGIN      
        1 taagaggtgg aaaattgaaa atcgattatc tagaactaat taatgaaata gcaaagtata
       61 aaactggtga ggaaatagaa atcctgagag acgtatatga tcaactcgaa gaagctggaa
      121 tcgaaggaat taataatgat cgttcgagtt ggagtaaact cagatactat ttcgcactct
      181 atatcgatac aacacaatta agaaatttag cttatacaaa attactattt gttgattgcg
      241 ttaaaggatt gcaaaaacat cttagtgaac ttgagcaggt gtaatcagat ggatctaaaa
      301 acatttacag cacagatcga actaatgcat caagaagctt taagacaaag cgcctcgtac
      361 gaagacaagt ggctcaacac gttccatggt ggacgtgaga gcgcacttga tcaagtactc
      421 aaattattga aaggggaacg tcgggatgga taagaaagcg gcaatgaaac gaatcattga
      481 actgacgcat tctgaaagtt ggcaggaaga tgaagaagtg gttgctgaag ttcagcgact
      541 aggaaagatc gctaatggac gtatacgaaa aaagccagac aaaagaaaac agcgcaaaag
      601 gaaaatcgtc gttgttttac acgaaggcaa aattttgatg caaggaacag ctagtgagct
      661 gtctgcagaa actggatata cgcgtgggac tattcgaacg tacgcttggc gaaatcacac
      721 cgatcgaaaa gggcacgaat ataagtattt ggaggaagaa taatgaacga aaacaaatta
      781 atcaaattgg gtgtagcagg agcagtaatc gtaggtattg gagttatcgg aggatttaag
      841 ttcttcgaaa aaatcgataa tggatatgtg ggtgtgcgct attcaatgaa cggcggtatc
      901 aaagatgaag cactgacgca aggtgtgaaa tttgtaggga ttgacaaagt gatccaatat
      961 ccaattcgct tgcaaactat ccaatcaaaa aatatttcag tatctacaag cgacggcaaa
     1021 aagacaacga ttgatatcaa atatgactac aaagttgatt caactaaagc agcaaaaatg
     1081 tacaaggaat ttgggaatat cacttcggaa gatatcgaaa gtggatggtt aaaatctaag
     1141 cttcaaaagg tcgctcgtga agtttatgcg aaatatagtc tgcttgatgt cctttcagga
     1201 gattcctcta aagttgaagc tgaggtatta acgaactttg ctaaatcagt tgaatctaaa
     1261 gggtttgaag tcgaagacgt aacacttggt gttccagatg tcgataaaga aacacaaaaa
     1321 tcaatcgatg cgatcattcg agctggtcaa gaaaatgaaa aagcgaagct agatgcagaa
     1381 actgcaaaaa ctcaagctga tagtgaagct tacaagaaaa caaaagctgc agaagcagag
     1441 gcagaatcta atcgcaaagt cgccgaatca gtaacagaca atttgattcg ttatgaagaa
     1501 gctcaagctc gcaaaaagca tggatgggta acagtaaatg gagcagatac tgtagttacg
     1561 gatgaagcag gcaaataata tgggattctt tatggctaaa attctcttgt tcttaggttt
     1621 agtaggagca gcatatctcg tgtatgcgct cctttcccaa actgatgaca aagaagatga
     1681 caacaacgat gaaatgaaat tttaggagga gaaataatgg atctcattac acaatacagt
     1741 gatatcatcc tcaagaaaat catggcaaag attcagaaag ataaaaaa
//
LOCUS       DASHHB010000156         1726 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.168, whole genome shotgun sequence.
ACCESSION   DASHHB010000156 DASHHB010000000
VERSION     DASHHB010000156.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1726)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1726)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1726
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.168"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            34..1557
                     /locus_tag="U2K71_002901"
     CDS             34..1557
                     /locus_tag="U2K71_002901"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002319360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEM7316896.1"
                     /translation="MDPTVFEAEEHIQKVIHKMFRPDNAYTKFDFRNGRPTKSAILTT
                     SSIDMAKKYYRAIKEMTKESDWLIKEFPNQPIREGRTMEDPDFPRIAITYSLEENSRD
                     SSAQQEEMQKIIEEYNQYYDTAWSLADIERYNGDINNRLARKRAEFKQFGKQIDLVIV
                     VDRLLTGFDAPTIQTLFVDRNLEYAGLIQAFSRTNRTYPEKTKGLIVTFRKPATMEKN
                     VEDATKLYSEAKEESGLIYPSYQESKKRFKQAYGKLKEVSSMESIDEHTPLEIRIEYV
                     KAFQELNRAYESLVTYDDYNDDMVQSSTLNNQIQLLEEQIGVYETVKGSLMEEEPEKE
                     GEDFSTIEFYSENTTKLYDIDSTYIDQLLGTYAANSSDAREEIEKALAKLNKTEGVKE
                     VYRQILNAFDVGTLDSNEDIFVIKRRYFTQASDNLIHAFSNEWFVSEKELHASNLQYM
                     PGEDPIPNMKAIINSKDYEGYKAKHPEAKPFKYPQEMKRAWRKMLDDELIPLENELR"
BASE COUNT          623 a          276 c          351 g          476 t
ORIGIN      
        1 gatcaaatgg agtctgtcaa aaaagaaagt tatttggacc ctactgtttt tgaagcagag
       61 gaacatatcc aaaaagtcat tcataaaatg ttccgtccag acaatgccta tacaaaattt
      121 gattttcgaa atggccgtcc aacgaagtct gcgatcttaa ccacgagttc cattgatatg
      181 gccaaaaagt attatcgggc catcaaagag atgacgaaag aatctgattg gttaataaaa
      241 gagtttccca atcagcctat ccgtgaagga cgaacgatgg aggatcctga tttcccacga
      301 atcgctatta cctattcatt agaagaaaat agccgagatt caagtgccca acaagaagaa
      361 atgcaaaaaa ttattgagga atataatcag tattatgata ctgcctggtc actagcagac
      421 attgagcgtt ataacggaga catcaataat cgtttggcac gaaaaagagc ggaattcaaa
      481 caatttggga aacaaatcga tttagtaatc gtagtggatc gtctattgac aggttttgac
      541 gcccctacga ttcaaaccct atttgtggat cgtaatctag agtatgcagg attgatccaa
      601 gccttttcac gtacgaaccg tacgtatcct gaaaaaacaa aaggactaat tgttactttc
      661 cgaaagccag cgacaatgga gaagaatgtc gaggatgcga caaaactgta ttcagaagct
      721 aaagaagaat cgggtttgat ttatccaagt taccaagaat ccaaaaaaag attcaagcaa
      781 gcatatggta agttaaaaga ggtttcatcc atggaatcca ttgatgaaca tacgccactt
      841 gaaatccgta tcgagtacgt caaagcattt caagaattga atcgtgcgta tgaatcgtta
      901 gtgacttatg atgattacaa tgatgacatg gtgcaatctt caactttgaa caatcaaatt
      961 caacttttgg aagaacaaat tggcgtgtac gagaccgtga agggttcatt gatggaggaa
     1021 gaaccagaaa aagagggaga agatttttca acgatcgaat tttatagtga aaataccacg
     1081 aagttgtatg atattgattc tacttatatt gatcaattac taggaactta tgctgcgaat
     1141 agttcagatg cacgagaaga aattgaaaaa gctttggcta aattgaataa aacagaagga
     1201 gtcaaagaag tttaccgaca aattttaaat gcatttgatg tggggacttt agatagtaat
     1261 gaagatatct ttgtcattaa acgtcgctat tttacacaag caagtgataa tttgattcat
     1321 gccttttcca atgagtggtt tgtttctgag aaagaattac atgcttctaa tctgcaatat
     1381 atgccaggtg aagatccaat tcctaatatg aaagcaatta tcaacagtaa ggattacgaa
     1441 ggatataaag ccaaacatcc agaagcgaaa ccatttaaat acccacaaga aatgaagcga
     1501 gcatggcgta aaatgttaga tgatgagttg attccacttg aaaatgaatt gaggtaaacg
     1561 agaagagtta agaaaattgt acgattcaaa gatagtagta aagtggaagt gtagaaaata
     1621 gggatagatg tctttttact tgttcacatt ttataacgga gtatttaaga aagctattga
     1681 ataatgattc ttccatcttt ttcattcatt taccggttct gttgca
//
LOCUS       DASHHB010000157         1674 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.169, whole genome shotgun sequence.
ACCESSION   DASHHB010000157 DASHHB010000000
VERSION     DASHHB010000157.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1674)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1674)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1674
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.169"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(333..>1674)
                     /locus_tag="U2K71_002902"
     rRNA            complement(333..>1674)
                     /locus_tag="U2K71_002902"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT          394 a          478 c          365 g          437 t
ORIGIN      
        1 cggttgggtt ttgatccttc ttctagcgat agaagttaga tcaagaaaat aagcaattga
       61 acttattaaa aaactctatc cagctcacaa atcagcctgt acggcaaaaa gatcgatcgt
      121 ctcataaaat caaagatttt atggccgtcc gcctattttg cttcaggcta aacaatttgt
      181 tcagctttca aattcaacgc ggtgttctcg gtttgtatta cttacattta cttcaaatat
      241 ccagttttca atgaacaaaa gtgtttgaga gtaaacctct caaaactgaa caaagtaaaa
      301 acaaattgtg tagcctccgt aatattcctt agaaaggagg tgatccagcc gcaccttccg
      361 atacggctac cttgttacga cttcacccca atcatctatc ccaccttagg cggctggctc
      421 caaaaggtta cctcaccgac ttcgggtgtt acaaactctc gtggtgtgac gggcggtgtg
      481 tacaaggccc gggaacgtat tcaccgcggc gtgctgatcc gcgattacta gcgattccgg
      541 cttcatgcag gcgagttgca gcctgcaatc cgaactgaga gaagctttaa gagattagct
      601 tagcctcgcg acttcgcaac tcgttgtact tcccattgta gcacgtgtgt agcccaggtc
      661 ataaggggca tgatgatttg acgtcatccc caccttcctc cggtttgtca ccggcagtct
      721 tgctagagtg cccaactgaa tgatggcaac taacaataag ggttgcgctc gttgcgggac
      781 ttaacccaac atctcacgac acgagctgac gacaaccatg caccacctgt cactttgccc
      841 ccgaagggga agctctatct ctagagtggt caaaggatgt caagacctgg taaggttctt
      901 cgcgttgctt cgaattaaac cacatgctcc accgcttgtg cgggcccccg tcaattcctt
      961 tgagtttcaa ccttgcggtc gtactcccca ggcggagtgc ttaatgcgtt agctgcagca
     1021 ctgaagggcg gaaaccctcc aacacttagc actcatcgtt tacggcgtgg actaccaggg
     1081 tatctaatcc tgtttgctcc ccacgctttc gagcctcagc gtcagttaca gaccagagag
     1141 ccgccttcgc cactggtgtt cctccatata tctacgcatt tcaccgctac acatggaatt
     1201 ccactctcct cttctgcact caagtctccc agtttccaat gaccctcccc ggttgagccg
     1261 ggggctttca catcagactt aagaaaccgc ctgcgctcgc tttacgccca ataaatccgg
     1321 acaacgcttg ccacctacgt attaccgcgg ctgctggcac gtagttagcc gtggctttct
     1381 ggttagatac cgtcaaggga tgaacagtta ctctcatcct tgttcttctc taacaacaga
     1441 gttttacgat ccgaaaacct tcttcactca cgcggcgttg ctcggtcaga ctttcgtcca
     1501 ttgccgaaga ttccctactg ctgcctcccg taggagtttg ggccgtgtct cagtcccaat
     1561 gtggccgatc accctctcag gtcggctatg catcgtggcc ttggtgagcc gttacctcac
     1621 caactagcta atgcaccgcg ggtccatcca tcagcgacac ccgaaagcgc cttt
//
LOCUS       DASHHB010000158         1623 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.170, whole genome shotgun sequence.
ACCESSION   DASHHB010000158 DASHHB010000000
VERSION     DASHHB010000158.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1623)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1623)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1623
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.170"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            53..>1623
                     /locus_tag="U2K71_002903"
     CDS             53..>1623
                     /locus_tag="U2K71_002903"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I restriction endonuclease subunit R"
                     /protein_id="HEM7316897.1"
                     /translation="MKISHREEAEVEEQLIRVLGEGHNQWTYRPDLKSEEDLWVNLRQ
                     KIISNNQAELNDFPLTDKEFETIKTELLLRTKTPFDAAKWLKGENGMARITIEREDPQ
                     LGSVSLILYSNQDIGGGISTYEVVHQIAKRGSNIEARDRRFDVTLLINGLPIVQIELK
                     QVTAKDSFYQAFNQIKKYAEEGMFRNNIFSTLQLFVVSNEQTTRYFANALPKDLHPKF
                     LFSWRTKDNKKVENLYEFCKQVLNIPDAHRLIADYTIVSEDQDNKILMVLHPYQVHAI
                     QALFIAANKHQSGYVWHATGSGKTLTSFVSTKLLARKSGIDRTIMLVDRKDLDNQTTT
                     EFTKFASEFNTGISSGNAKANSLIVGTGSAKELSETLLADANANVVIITTRQKLDAAL
                     KYAKKQEEKKGTNRFQKLMGQHIVFVVDECHRALSAENMEEIKKMFPKSTWFGFTGTP
                     IFPENRKQAKGQLARTTHDQYGEVLHTYTIKNALEDGSVLGFQVEHENTVEPTSLENK
                     IYRKLKEVETYAEYSP"
BASE COUNT          560 a          282 c          358 g          423 t
ORIGIN      
        1 gaaacgaaaa aaggcttctt acaaaagatg tttgtttagg gagggaagat aaatgaaaat
       61 tagtcaccgt gaggaagcgg aagtcgaaga acagttgatc cgtgtattgg gggaagggca
      121 taatcagtgg acgtatcgtc ccgatctaaa atcagaagaa gatctttggg tcaatttacg
      181 ccaaaaaatc atctcaaata atcaagcaga actgaatgat tttcctttga ccgataaaga
      241 atttgaaacg atcaaaacag aattgttact acgaacgaaa accccctttg atgctgcgaa
      301 atggttaaaa ggtgaaaatg gaatggctcg gatcacgatt gaacgggaag atccgcaact
      361 gggttctgtc tcgctcattc tttattctaa tcaagacatt ggtggaggaa tctctaccta
      421 tgaagtggtc catcaaattg caaaacgtgg aagtaacata gaagcacggg atcgacgttt
      481 tgacgtcacc ttactgatca atggattacc gattgtccaa atcgagctga aacaagtaac
      541 cgcaaaagat agtttttatc aggcgtttaa ccaaatcaaa aaatatgcag aagaaggtat
      601 gtttcggaac aatatctttt cgaccttaca gttgtttgtg gtttccaatg aacaaaccac
      661 tcggtatttt gcaaatgcct taccaaagga tttgcatcct aagtttttat ttagttggcg
      721 aacgaaagat aataaaaagg tagaaaactt atatgagttt tgcaaacaag tgctaaatat
      781 cccggatgct catcgcttga ttgcggatta tacgattgtc agtgaagatc aagacaataa
      841 aatcctcatg gtgttacatc cttatcaagt acatgcgatt caagccttgt ttattgctgc
      901 aaataaacat caatcaggat atgtgtggca tgcgactggt tcaggaaaaa cgttgactag
      961 ttttgtatct actaagcttt tagcacgaaa atcggggatc gatcggacga tcatgttagt
     1021 ggaccgcaaa gatttagata atcaaaccac cacagagttt acgaaatttg cttcggaatt
     1081 taatactggg atttcttccg ggaacgccaa agcgaatagc ttgatcgtgg gaacaggaag
     1141 tgccaaagag ttaagtgaaa ctctgttggc agatgccaat gcaaatgttg tgattattac
     1201 aacccgtcaa aagctagatg cggcgttgaa atatgcaaaa aaacaagaag agaaaaaagg
     1261 aaccaatcgc ttccaaaaat taatggggca acatatcgtg tttgtagtag atgagtgtca
     1321 tcgagcgtta agtgctgaaa atatggaaga aatcaaaaaa atgtttccga aatccacttg
     1381 gtttggcttt acaggtacac cgattttccc tgagaatcgg aaacaagcca aagggcaatt
     1441 ggcacgtacg acccatgatc agtatggaga ggtcttgcat acctatacca tcaagaatgc
     1501 attagaagat ggctcggttt taggatttca agtagagcat gaaaacaccg tggagccgac
     1561 ttcattagag aataaaatct atcggaaatt aaaagaagta gaaacatatg cagagtattc
     1621 acc
//
LOCUS       DASHHB010000159         1576 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.171, whole genome shotgun sequence.
ACCESSION   DASHHB010000159 DASHHB010000000
VERSION     DASHHB010000159.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1576)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1576)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1576
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.171"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            123..1292
                     /locus_tag="U2K71_002904"
     CDS             123..1292
                     /locus_tag="U2K71_002904"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HEM7316898.1"
                     /translation="MTKFTIEIMQTLLNKGDLDELFQDHLERAINSLLQAELTAFLDY
                     EKYDRNGFNSGNSRNGNYSRTFKTEYGELNLTIPRDRNGEFSQQTLPAYKRTNDSLET
                     TIIQLFQKGITMAEISKLIEKMYGHHYTPQTISNMSKLVAEDVLAFKERTLEANYSVI
                     FMDATHIPVKRQTVSKEAVYITIGIRLDGTKEVLGFTIAPTESAYIWKEVLQDLRKRG
                     LEEVLLVVTDGLSCIEESIHSVYPNAQFQQCCVHVSRNIAHKVRVRGRKEICEDFKLV
                     YQANSKEEALDHIDFMTRKWKKQYPRVVNLLLNPALLTFYNFPHAIRRTIYSTNLIEG
                     FNKQLKRYTRRKEQFPNEESLERFFISQFNQYNQKFLGRIHKGFKEIQDTLESMI"
BASE COUNT          560 a          255 c          258 g          503 t
ORIGIN      
        1 ttttacactc tctattatga tagcgtaaaa tattttgtgt aaatagaaat ttagggtaga
       61 aaaaaaagtc cattctgtaa aattaaagtt accacacaga aattttatag gaggactttc
      121 tcatgactaa atttactata gaaattatgc aaacactact caataaaggg gatttagatg
      181 aattatttca ggatcattta gagcgagcga ttaattcact tctacaagca gagctaactg
      241 cttttttaga ttacgaaaaa tatgatcgaa atggattcaa ttcaggaaat tcccgtaacg
      301 ggaattattc acgtacattt aaaacagaat atggcgagtt gaatttaact attcctagag
      361 atcggaatgg cgaattttct caacaaacac taccagcata taagcgaacc aatgattcat
      421 tagaaacgac cattatccaa ttatttcaaa aaggcattac tatggctgaa atctccaaat
      481 taattgaaaa aatgtatggt caccactata cgccacaaac aatctccaac atgagtaaat
      541 tggtggctga agatgtttta gcttttaaag aaagaacgtt agaagccaat tattccgtta
      601 tatttatgga tgcgacgcat atccctgtga agcgacagac tgtttcgaag gaagcagtgt
      661 atattacgat tggtattcgt ttggatggaa ccaaagaagt tcttgggttt actattgccc
      721 caactgagtc tgcttatatt tggaaagaag ttcttcaaga tcttagaaaa cgtgggttag
      781 aagaagtttt attagtagtg acagacggat taagctgtat tgaagaaagt atccatagtg
      841 tgtatccgaa tgcccaattt caacaatgtt gtgtgcatgt atctagaaat atcgctcata
      901 aagttcgtgt tcgaggtcga aaagaaattt gtgaggattt caaattggtt taccaagcga
      961 attcaaaaga agaggcattg gatcacatcg actttatgac taggaaatgg aaaaagcagt
     1021 atccaagagt cgtcaattta ctcttgaatc ctgccctatt aaccttttat aatttccctc
     1081 acgccatcag acgaacaatt tattcgacga acctgattga aggctttaat aagcagctaa
     1141 aacgatatac tcgaagaaaa gaacaattcc ctaatgaaga atctctagag agattcttca
     1201 tttctcaatt taatcaatat aaccaaaaat ttttaggtag aattcacaag ggatttaaag
     1261 aaattcagga tacattagag tcgatgattt aactgtaatt aacagaatgg attttccatt
     1321 tacatataat tcttgacgct accataatct aaataaccat catattcatc tactacttat
     1381 ataattgagt tttattaaac tttttaagtt tctattctat caaatattta aacaatctct
     1441 gcatccgaaa agactacctc gtatagtttt actaacaata tttttattag cgtgttcaaa
     1501 tcctgatttc ttgtatcaat tattttctgt tcactttcaa taagaaaaaa gtctttactt
     1561 ccatggataa taataa
//
LOCUS       DASHHB010000160         1475 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.172, whole genome shotgun sequence.
ACCESSION   DASHHB010000160 DASHHB010000000
VERSION     DASHHB010000160.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1475)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1475)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1475
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.172"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..358
                     /locus_tag="U2K71_002905"
     CDS             <1..358
                     /locus_tag="U2K71_002905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002340820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1071 domain-containing protein"
                     /protein_id="HEM7316899.1"
                     /translation="NQPVVKPNAMQINNSLKRCFVKALALHGLGLYVFQGEDIPTPPR
                     IDTKKLNMLETILEAFNEQMGKDMTKTLIEYVNEQTDKLGLLADNVETIEQLSYEQCA
                     LMERAIAAKRKELDKK"
     gene            364..1050
                     /locus_tag="U2K71_002906"
     CDS             364..1050
                     /locus_tag="U2K71_002906"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316900.1"
                     /translation="MFKPLIDSYSAVLKKFKGKDISATINEEVNIDRLKTMYDGYDGD
                     RVIEIRFIDPRRFTVQQRNFIYALIGDIFIDTGMPTDFWKEFFYFRFEGVTGRKISLK
                     DESNTTVSDANVLANIILDFIFEHHIPFKEGYEILPANQEYYFYKCITKRVCCICGKT
                     GADIDHFDKALGRRKRKEVDHSEYTFAALCRIHHTEKHKIGVINFKNKYQIKGIKLNQ
                     KTIKKLNIGG"
     gene            1053..>1475
                     /locus_tag="U2K71_002907"
     CDS             1053..>1475
                     /locus_tag="U2K71_002907"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7316901.1"
                     /translation="MTEHRSYYAIIPANVRYDKRLKPNTKLLYGEITALCNERGFCWA
                     GNEYFADLYGVNKETISRWVSDLIKFGYLNREIIYKEGTNQIINRYLRINQYPIDEKR
                     NTPIDEKVKDNNTSINNTFNNTKEYIRELPPSKKSKAKP"
BASE COUNT          560 a          249 c          293 g          373 t
ORIGIN      
        1 aaaccaacca gttgttaaac caaatgcaat gcaaatcaat aactcattga agcgatgctt
       61 tgtgaaagca ttggctctac acggactggg attatatgta tttcaagggg aagatattcc
      121 aacaccacct agaatcgata caaagaaatt aaacatgcta gagacgattc tagaagcttt
      181 caatgagcag atgggtaaag atatgaccaa aaccttaatc gaatatgtta atgagcagac
      241 agataaatta gggctcttag ctgataacgt tgaaactatt gaacagttaa gctatgagca
      301 atgtgccttg atggagcgag caatagcagc taagagaaaa gaattagata agaagtgata
      361 tgagtgttta aaccattaat cgattcatat tcagcggttc tgaaaaagtt caaaggaaaa
      421 gacataagcg caaccatcaa tgaggaagtg aacattgatc gactaaagac gatgtatgac
      481 ggctacgatg gtgatcgagt cattgaaatt cgttttattg atcctagacg tttcaccgta
      541 cagcaacgaa acttcatcta tgcgctgata ggcgatattt ttatcgatac aggcatgcca
      601 acggacttct ggaaggaatt cttctacttt cgttttgaag gtgtcacagg gcgcaaaata
      661 agcctgaaag acgaatcgaa tacaactgtg agtgatgcca atgtcttagc aaatatcatc
      721 ttagatttca tctttgaaca tcatattcct ttcaaagaag gctatgagat tttaccagcg
      781 aatcaagaat attacttcta caaatgcatc acaaaaagag tctgctgcat ctgtggcaaa
      841 acaggagctg acatcgatca ctttgacaaa gcgctaggaa gacgaaagcg caaagaagtt
      901 gatcattcag agtacacatt tgcagcactc tgcagaatcc atcacacaga gaagcacaaa
      961 ataggtgtga ttaatttcaa aaataaatat caaatcaaag ggatcaaatt aaaccagaaa
     1021 acaatcaaaa agttaaatat tggagggtaa aaatgacaga acatcgaagt tattatgcga
     1081 ttataccagc caacgtaagg tacgacaaaa gacttaaacc aaatactaag ttgttatacg
     1141 gagagataac ggccttgtgt aatgaaagag gcttttgttg ggcaggcaat gagtactttg
     1201 cagatttata tggtgtgaat aaagagacca tatcgcgatg ggtaagtgat ttgattaagt
     1261 ttggatactt gaatcgggaa atcatttaca aagagggtac caatcaaata atcaataggt
     1321 acctacgaat taatcaatac cctattgacg aaaaacgcaa tacccctatt gacgaaaaag
     1381 tcaaagataa taatacatct attaataata catttaataa tacaaaagaa tatataagag
     1441 agttaccgcc ttcgaaaaaa tcgaaggcta agccc
//
LOCUS       DASHHB010000161         1444 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.173, whole genome shotgun sequence.
ACCESSION   DASHHB010000161 DASHHB010000000
VERSION     DASHHB010000161.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1444)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1444)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1444
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.173"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..297)
                     /locus_tag="U2K71_002908"
     CDS             complement(<1..297)
                     /locus_tag="U2K71_002908"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000221326.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7316902.1"
                     /translation="MTYKHLTIDELTMIESYYLQHNKPVEIANRMGRAIQTIYNVVNK
                     FKQGKTALDYWHQYKENKKKCGRKVIQLPAHEVDYIKEKVTLGWTPDVIIGRKER"
     gene            483..1418
                     /locus_tag="U2K71_002909"
     CDS             483..1418
                     /locus_tag="U2K71_002909"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002372379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HEM7316903.1"
                     /translation="MKLWMKILLGVALALIVALVFAGNYFYNYAVVPSEKDFLDEDTP
                     GTTQTSKETDAHKWFSSKENRSDWTITSKDGLKLSAIYLPAETKSEKTALVAHGYMGN
                     AETMTNYAKMFHDMGYNVLVPDARGHGKSEGDYIGFGWPERKDYVQWINKVLENNGKS
                     QEIVLYGVSMGAATVMMTSGEKLPDNVKAIIEDCGYSSVHDELAYQLDDMFSLPAFPL
                     MQVTSLVTKVRAGYFFGEANAVEQLKKNQRPMLFIHGDEDTFVPFEMLDKVYRATKGP
                     KEKYVVKGAEHAEAYKTDPEKYQQVVQQFLSQYVH"
BASE COUNT          464 a          240 c          302 g          438 t
ORIGIN      
        1 cctttctttt cgcccgataa tgacgtcagg cgtccaacca agagtgactt tctctttaat
       61 gtaatctact tcatgagcag gtaattgaat gacttttcta ccacattttt tcttattttc
      121 tttatactgg tgccaataat caagagcagt cttgccttgc ttgaacttat tgactacatt
      181 ataaatagtt tgtatagcac gtcccattcg gttagcgatt tcaaccggct tattatgttg
      241 aagataatat gattctatca ttgtcagttc gtctatggta agatgtttgt aggtcattta
      301 tggttacact cctttgtttt ctttcgtcgg aaatacaatt tgagtgtacc ataaatgcct
      361 ttttattttt ctaacttaat tttacaattc gcgggttata aaaatgaaca ttgcatagta
      421 gagattcttt gattatacta attcaaggtt atatgaaaaa agagaaatga gatggatgat
      481 ttatgaaact ttggatgaaa atccttttag gcgtcgcttt agctctgatt gttgcgttag
      541 tgtttgcagg gaattatttt tataattatg ctgttgtgcc ttctgagaaa gactttttag
      601 acgaagatac acctggtaca acacagacat ccaaagaaac ggatgcacat aaatggttta
      661 gttcaaaaga aaaccgaagc gattggacca tcacttcaaa agacggtctg aaattatcag
      721 cgatttatct tcctgctgaa acgaaaagtg aaaaaacagc tcttgtggct catggctata
      781 tgggaaacgc tgaaacgatg accaattacg caaagatgtt tcatgatatg ggttacaatg
      841 tccttgttcc tgatgcaaga ggacatggaa aaagcgaagg ggattacatt ggatttggat
      901 ggccagagcg taaagactat gtacagtgga taaacaaagt gctagaaaac aatggaaaat
      961 cgcaagaaat cgttctatac ggtgtcagca tgggagcagc aacagttatg atgactagcg
     1021 gagaaaaact accagataat gtgaaagcaa tcattgaaga ttgcggttat tcttccgttc
     1081 atgatgagct tgcttatcag ctagacgata tgtttagtct tccagcattt ccgcttatgc
     1141 aagtaacgag tctagtgaca aaagtccgtg ctggctactt tttcggtgaa gcaaatgcag
     1201 tggaacaatt gaagaaaaac cagcgcccaa tgctctttat acatggagac gaagatacat
     1261 ttgtaccatt tgaaatgtta gataaggtct atcgtgcaac gaagggacca aaagaaaagt
     1321 atgttgtaaa aggagccgag catgcggaag catacaagac agatcctgaa aaatatcagc
     1381 aagtagtcca acaattttta agccaatatg ttcattgaac gagtttaaaa gtaggggatg
     1441 aaaa
//
LOCUS       DASHHB010000162         1437 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.174, whole genome shotgun sequence.
ACCESSION   DASHHB010000162 DASHHB010000000
VERSION     DASHHB010000162.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1437)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1437)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1437
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.174"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            15..284
                     /locus_tag="U2K71_002910"
     CDS             15..284
                     /locus_tag="U2K71_002910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002352161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316904.1"
                     /translation="MKIDYLELINEIAKYKTGEEIEILRDVYDQLEEAGIEGIKNDRS
                     SWSKLRYYFALYIDTTQLRNLAYTKLLFVDCVKGLQKHLNELEQV"
     gene            289..453
                     /locus_tag="U2K71_002911"
     CDS             289..453
                     /locus_tag="U2K71_002911"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316905.1"
                     /translation="MDLKTFTAQIELMHQEALRQSVSYEDKWLNTFHGGRESALDQVL
                     KLLKGECRDG"
     gene            446..748
                     /locus_tag="U2K71_002912"
     CDS             446..748
                     /locus_tag="U2K71_002912"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316906.1"
                     /translation="MDKKAAMQRIIELTYSEDWQNDKEAASEVMRLGRAMWADKSNKP
                     RPRKIAIWHGDKLLVIGTAEQLASLTGLHEKIVRKRARCGYTDAKKRTFRYVEESS"
     gene            745..906
                     /locus_tag="U2K71_002913"
     CDS             745..906
                     /locus_tag="U2K71_002913"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010752308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEM7316907.1"
                     /translation="MTTEEVIQMRIRSLQREIDDLERTKAVMVNETARKAIDLHIENL
                     RREIHRLEE"
     gene            903..1208
                     /locus_tag="U2K71_002914"
     CDS             903..1208
                     /locus_tag="U2K71_002914"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016628584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316908.1"
                     /translation="MSVDKKAAMKRIAELTKSESWQEDKEIVAEVQKLGKSMWTEKPK
                     RRTPRKIAIWHDDRILVTGTAEQLSEITGLSKNIIWDRARSLWIDSKGRQFRYVEER"
     gene            1208..>1437
                     /locus_tag="U2K71_002915"
     CDS             1208..>1437
                     /locus_tag="U2K71_002915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1140 family protein"
                     /protein_id="HEM7316909.1"
                     /translation="MDLITQYSDIILKKIMMKIQKDKKSKERAELVKLEMAETGAGVR
                     SSRHWKAAANIEFYYNEIQKGFDQMRELDRQT"
BASE COUNT          553 a          220 c          354 g          310 t
ORIGIN      
        1 taagaggtgg gaaattgaaa atcgattatc tagaactaat taatgaaata gcaaagtata
       61 aaactggtga ggaaatagaa atcctgagag acgtatatga tcaactcgaa gaagctggaa
      121 tcgaaggaat taagaatgat cgttcgagtt ggagtaagct cagatactat ttcgcacttt
      181 atatcgatac aacacaatta agaaatttag catataccaa attactattt gttgattgtg
      241 ttaaaggctt gcaaaaacat cttaatgaac ttgagcaggt gtaatcagat ggacctaaag
      301 acatttacag cacagattga actaatgcat caagaagctt taagacaaag tgtatcgtac
      361 gaagacaagt ggctcaacac gttccacggc ggacgtgaga gcgcacttga tcaagtactc
      421 aaattactga aaggagaatg tcgggatgga taagaaagcg gcaatgcagc gaattatcga
      481 attgacttat tcagaagatt ggcaaaatga caaagaagct gcttcagaag tgatgaggct
      541 tggaagagcg atgtgggcag acaagagcaa caagccaaga ccacgaaaaa tcgcaatttg
      601 gcacggtgac aaacttctag tgatagggac agctgaacag ttagcaagtc tcacaggctt
      661 gcacgagaaa atcgtgagga aaagagcaag gtgtggctac acagacgcta agaagagaac
      721 gtttagatac gtggaggaat catcatgaca acagaagaag tgattcaaat gcgtattcga
      781 agccttcagc gtgagattga cgatctggaa cgaacaaagg cagtgatggt caatgaaacg
      841 gcgagaaagg caatcgattt gcacatagag aatttaagaa gggaaatcca tcgattggag
      901 gaatgagcgt ggataagaaa gcagcaatga aaagaattgc tgaattaacc aagtcagaat
      961 cttggcaaga agacaaagaa atagttgcag aagtccaaaa gctcggtaaa tcaatgtgga
     1021 ctgaaaagcc caaacggaga acgccgagaa aaattgcaat ctggcatgat gaccgaattt
     1081 tagtaacagg tactgctgaa cagttatctg aaattactgg attaagcaaa aacattatct
     1141 gggatagagc taggagctta tggattgatt caaaaggacg acagtttagg tatgtggagg
     1201 agagataatg gatctcatta cacaatacag tgacatcatc ctcaagaaaa tcatgatgaa
     1261 gattcagaaa gataaaaaat caaaagaacg agctgaatta gttaagttag aaatggctga
     1321 aacaggagca ggagtgcgaa gtagcaggca ttggaaagca gcagcaaaca ttgaatttta
     1381 ttacaacgaa attcaaaaag ggttcgatca gatgcgtgag ctggatcggc aaacaaa
//
LOCUS       DASHHB010000163         1424 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.175, whole genome shotgun sequence.
ACCESSION   DASHHB010000163 DASHHB010000000
VERSION     DASHHB010000163.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1424)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1424)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1424
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.175"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(150..1337)
                     /locus_tag="U2K71_002916"
     CDS             complement(150..1337)
                     /locus_tag="U2K71_002916"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element IS16 family transposase"
                     /protein_id="HEM7316910.1"
                     /translation="MTQVHFTFESEEIQAIINESGANDTAKTLMTIMFNQLMEEQRNQ
                     YIQANAYERSEERQSQRNGYYDRSFTTRIGTLELHVPRTRDGKFSPTIFERYQRSEKA
                     LIAAMIEMVISGVSTRKVTKTVELLTDGATVSKSFVSNLMKQLDPFVFEWRNRSLEGS
                     EYPFFMCDALYMKVRENHRIVSKGVYIGIGIDSDGRRTILGFDVQDGESEDNWDTVFQ
                     SFVQRGLFGVKLVISDAHKGLVKAVRKNFLGASWQRCQAHFLRNIFDKLPKKVSSDVK
                     DELKSIFKASELELTRERKEHFLEKYGCDSKLSAACDILENGFEDAIQILSFPENIRR
                     RIRTTNVLERLNEEIRRRERVIRIFPNINSITRIIGTLLMEKDTEWLASPRKYLEFNS
                     NNI"
BASE COUNT          414 a          309 c          249 g          452 t
ORIGIN      
        1 taaaagttca acctcatcaa aagcacaatg ttttgcgttg tgaacctaac tgccatgcct
       61 ttctttctga ggagagaggt caggagcagt agcgcagcga ctcttgatgt ctctcctcag
      121 aaagaagaga ctcgcacagg ttcatggtgc taaatgttat tcgaattaaa ttctaaatat
      181 tttcgtggag aagccagcca ctcagtgtct ttttccatca aaagtgttcc aattatccga
      241 gtgatggaat tgatatttgg aaaaatccga atcactcgtt ctctccggcg aatctcttcg
      301 ttcaaccgtt ccaacacatt ggttgtacga atccttcggc ggatattttc aggaaaagac
      361 aaaatttgaa tagcatcttc aaagccgttt tctaaaatgt cgcaagcagc acttaatttt
      421 gaatcacaac catatttttc caagaagtgc tcttttcttt cacgtgtcaa ttcaagttca
      481 gaggctttaa agatactctt caactcgtct tttacatcag aagaaacttt ctttggcagc
      541 ttatcaaaaa tatttctcaa aaagtgggct tggcatcttt gccagctcgc acctaaaaag
      601 ttcttgcgaa cagccttaac taaccctttg tgtgcatcag aaatgaccag ttttacaccg
      661 aacaaacctc gttgaacgaa cgattgaaaa acggtatccc aattatcttc cgattcgccg
      721 tcttgaacat caaaaccaag aatcgtacgt cgtccatcag aatcaatgcc gataccaata
      781 tatacacctt ttgagacaat acgatgattt tcccttactt tcatatacag ggcatcacac
      841 atgaaaaatg gatattccga gccttctagg ctccggtttc tccactcaaa aacaaatgga
      901 tccaactgtt tcatcagatt tgaaacgaat gatttagaaa cagtcgcacc atccgttagc
      961 agttcaactg ttttagttac tttgcgagtg gagacaccag aaatgaccat ttcaatcatg
     1021 gcagcgatca aagctttttc gcttcgctga tatctttcaa agatagtagg actaaactta
     1081 ccgtctctgg ttcgtggaac atgaagttct aacgtaccaa ttcgagttgt aaacgaacga
     1141 tcgtagtaac catttctttg tgattgccgt tcttctgaac gttcataggc atttgcctga
     1201 atatattgat ttcgttgttc ttccatcagc tgattaaaca tgatagtcat aagtgttttt
     1261 gcggtatcat tcgcaccgct ttcgtttata attgcttgaa tttcttcgct ttcaaaagta
     1321 aaatgtactt gagtcattga tatcagtcct attcgagtat gttttctgca aaaaacattg
     1381 tactcctaac tgagatcaat ggcttttttg atgcttcact ttta
//
LOCUS       DASHHB010000164         1374 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.176, whole genome shotgun sequence.
ACCESSION   DASHHB010000164 DASHHB010000000
VERSION     DASHHB010000164.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1374)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1374)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1374
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.176"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            39..1340
                     /locus_tag="U2K71_002917"
     CDS             39..1340
                     /locus_tag="U2K71_002917"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002351973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM7316911.1"
                     /translation="MPDLHHIKLLLGIKDKNIFITNVESKSIKNIKSLVVSATLSKEI
                     RRCPLCKQMNHEGMIVKNGKKKSLIQLNKCANQLTYLALAKQRYHCRGCHTYFTANTY
                     IVDRNCFIAKQVRYKILEELTEKQAMTTIAKHCGVSWSTVSRTLASLIPMTKVKRNWL
                     PRCLLMDEFRSLKNQVGPYSFSCMDGDTGKLLDILPSRKKKALVSYFMQFERRARLKV
                     KVIVTDMNVSYASLIKECFPKAKLVVDRFHIVKHLIRKFEDIRVRIMKSFDRNDPTQA
                     KYYRQLKALSRLLIKRQDKLVYDKWTKWRNFGWAYLTESEVVDRLLSISDELRIAYFY
                     YQEILQAFHDKEADTFFKLVRTMPNSVPKELHSIKKAFINYESGIRLALELPYSNAKI
                     ENLHTHIKALKRVAYGFRSFRKMKTRIFLLNNLITYESKNI"
BASE COUNT          448 a          275 c          253 g          398 t
ORIGIN      
        1 aattgaatca ccacaacaca atcaaaggag cgttctgtat gcctgatctt catcatatca
       61 aactactact tggaatcaaa gataaaaaca ttttcatcac aaatgtggaa tcgaagagca
      121 ttaagaatat caagagtctt gttgtttctg cgacgttaag taaagaaatt cgtcgctgtc
      181 ctttgtgtaa acagatgaat catgaaggca tgatcgttaa gaacggaaag aaaaaatcgc
      241 tcattcaact taataaatgt gcgaaccaac tgacttatct tgctttagcg aaacaacgct
      301 accactgtcg tggctgccac acctatttta cggcgaatac ctacattgtc gatagaaact
      361 gctttatcgc taaacaagtg cgctataaaa ttttagaaga gctaactgaa aaacaagcaa
      421 tgacgacaat tgctaagcat tgcggcgttt cttggtcgac cgtttctcgg acactcgctt
      481 ctctgatccc catgacaaaa gtaaaaagaa attggttacc gcgttgttta ttaatggatg
      541 aatttcgttc actgaaaaat caagtcggtc cctactcctt ttcttgtatg gatggcgata
      601 ctggaaaatt actggatatt cttccttcac ggaagaaaaa ggctctcgtt tcttatttta
      661 tgcaatttga acgacgcgca cgattgaagg taaaggtcat cgtgacggac atgaatgttt
      721 cttatgcctc tctgataaag gaatgttttc caaaggcaaa actcgttgtg gatcgttttc
      781 acattgtcaa acatctcatt cgtaaatttg aggatattcg cgtacgaatc atgaaaagct
      841 ttgatcgaaa cgatccaaca caagccaaat attatcgaca attaaaggcg ttgtctcggc
      901 ttctgatcaa acgacaggac aagcttgttt atgacaaatg gaccaaatgg cgcaattttg
      961 gttgggcgta tctcaccgaa agtgaagtgg tggatcgtct gctttcgatc tctgacgaat
     1021 tacgaatcgc ctatttttac tatcaagaaa ttcttcaagc gtttcacgac aaagaggcgg
     1081 acactttctt taaacttgta agaacgatgc ccaattccgt tccaaaagaa ctgcattcta
     1141 taaaaaaagc ttttataaac tatgaatccg gcattcgttt agccctcgaa ttgccttatt
     1201 caaatgccaa aatagaaaat ctgcataccc acatcaaagc attgaaacga gtcgcttatg
     1261 gctttcgaag ctttcggaaa atgaagacca gaattttctt actaaataat ctgattacct
     1321 atgaatcaaa aaacatctaa agtagaaaag gaattctacc ttagatgttc ttat
//
LOCUS       DASHHB010000165         1364 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.178, whole genome shotgun sequence.
ACCESSION   DASHHB010000165 DASHHB010000000
VERSION     DASHHB010000165.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1364)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1364)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1364
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.178"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            82..>1364
                     /locus_tag="U2K71_002918"
     CDS             82..>1364
                     /locus_tag="U2K71_002918"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa11 family transposase"
                     /protein_id="HEM7316912.1"
                     /translation="MNDSIKKMLRITEKDLMITEVSYETLQKKKTLVVDAVLSPTPRA
                     CRSCGSTVVDGNGKAIIVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKSCQVSLTTVIRTLKEFKSYLPKQSK
                     KILPRVLMVDEFRSHASIEDKMSFICADGETGKLIDVLPTRKLPRLTSYFLGCTNPEE
                     VEFLVTDMNAAYFQLTKRVLPNAKVVIDRFHIVKHMNQAFNELRIREMNELRKAGQKS
                     QAEKLKKNWRFLLKNRANINHYEYKTWKSFRAPKYPFLTEAMMIDRLLEFSPPLKEAY
                     PFFHELVEAFRDKDPDLFFSLLAELPETLDDSFREKLQNLLTYEEGITNAMIYPYSNG
                     KIEAKNTHIKTMKRVSYGFKSFENMRIRIFLINQL"
BASE COUNT          465 a          262 c          257 g          380 t
ORIGIN      
        1 attttaggta ttaaaaaaca tatcgttctc tagtatgatt aaatcaccac aaaataaaca
       61 actgagagga cgatacgcta tatgaatgat tctatcaaaa aaatgctgag aataacagag
      121 aaagatttga tgattacaga ggtctcttac gagacccttc agaagaaaaa gacgttggtc
      181 gtcgatgctg ttctctcgcc tactcctcgt gcttgtagaa gttgtggttc tactgtggta
      241 gatggaaacg ggaaagcaat tatagtgaaa aatggaaaaa aggaaacgat tgtccgtttt
      301 gaacaataca atcatatgcc tttggttatg cgcctaaaaa agcaacgcta tacctgtaaa
      361 aactgtcgca cccattggac ggctcaaagt tattttgtcc aacccagaca ttcaatcgca
      421 aatcatgtta gatataaaat tgcttcttta ctgactgaaa aagtatcttt atcttttatt
      481 gcgaaaagct gtcaggtatc tttgaccacc gttattcgta cattgaaaga gtttaaaagc
      541 tatttaccaa agcaatctaa gaagatcctc cccagagtat tgatggttga cgaatttcgt
      601 tcgcatgctt ccatagaaga taagatgagc tttatttgcg cggatggcga aacaggaaaa
      661 ttaatagatg ttttgcctac gcgtaaatta cctcgattaa caagctattt cttaggttgt
      721 accaatccag aagaagtaga attcttggtg acagacatga acgccgccta cttccagctc
      781 accaaacgtg ttctgccaaa tgcgaaagtc gtgattgatc ggtttcatat tgtcaaacac
      841 atgaatcaag cgttcaatga gttgcgtatc cgtgaaatga atgaacttcg taaagccgga
      901 cagaaaagcc aggcagaaaa actgaaaaag aactggcgct ttttgctaaa aaatcgtgca
      961 aacatcaacc attatgaata caaaacatgg aaaagtttcc gagcaccaaa atacccattt
     1021 cttactgaag caatgatgat tgatcgattg cttgagtttt ctccgcccct aaaggaggcg
     1081 tatccctttt ttcatgaatt agttgaagcc tttcgagaca aagaccctga cttatttttc
     1141 tccttattgg cagaacttcc cgaaacgtta gatgacagct ttcgggaaaa gcttcaaaac
     1201 cttctgacct atgaagaagg catcaccaac gcaatgatct atccttattc caatggaaaa
     1261 atagaagcga agaataccca cataaagaca atgaaacgag tatcctacgg atttaaatca
     1321 tttgagaaca tgagaattag aatctttttg atcaatcaat taat
//
LOCUS       DASHHB010000166         1355 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.179, whole genome shotgun sequence.
ACCESSION   DASHHB010000166 DASHHB010000000
VERSION     DASHHB010000166.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1355)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1355)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1355
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.179"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1355
                     /locus_tag="U2K71_002919"
     rRNA            <1..1355
                     /locus_tag="U2K71_002919"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          362 a          295 c          415 g          283 t
ORIGIN      
        1 caagctgtga tggggaggga aataatagta ccgaagttcc tgatgtcaca ctgccgagaa
       61 aagcttctag tgagaaaaca gcggcccgta ccgcaaaccg acacaggtag tcgaggagag
      121 aatcctaagg tgagcgagag aactctcgtt aaggaactcg gcaaaatgac cccgtaactt
      181 cgggagaagg ggtgctgatc atacgatcag ccgcagtgaa taggcccaag cgactgttta
      241 tcaaaaacac aggtctctgc aaaatcgtaa gatgaagtat aggggctgac gcctgcccgg
      301 tgctggaagg ttaagaggag tgcttagcgc aagcgaaggt acgaattgaa gccccagtaa
      361 acggcggccg taactataac ggtcctaagg tagcgaaatt ccttgtcggg taagttccga
      421 cccgcacgaa aggcgtaacg atttgggcac tgtctcaacg agagactcgg tgaaatttta
      481 gtacctgtga agatgcaggt tacccgcgac aggacggaaa gaccccatgg agctttactg
      541 tagtttgata ttgagtgtct gtaccgcatg tacaggatag gtaggagccg tagaaatcgg
      601 aacgctagtt tcgatggagg cgctggtggg atactacccc tgcgttatgg ccactctaac
      661 ccgcaccact aatcgtggtg ggagacagtg tcagatgggc agtttgactg gggcggtcgc
      721 ctcctaaaag gtaacggagg cgcccaaagg ttccctcaga atggttggaa atcattcgaa
      781 gagtgtaaag gcagaaggga gcttgactgc gagaccaaca agtcgagcag ggacgaaagt
      841 cgggcttagt gatccggtgg ttccgcatgg aagggccatc gctcaacgga taaaagctac
      901 cctggggata acaggcttat ctcccccaag agtccacatc gacggggagg tttggcacct
      961 cgatgtcggc tcgtcgcatc ctggggctgt agtcggtccc aagggttggg ctgttcgccc
     1021 attaaagcgg cacgcgagct gggttcagaa cgtcgtgaga cagttcggtc cctatccgtc
     1081 gcgggcgttg gaaatttgag aggagctgtc cttagtacga gaggaccggg atggacttac
     1141 cgctggtgta ccagttgttc tgccaagggc attgctgggt agctatgtag ggaagggata
     1201 aacgctgaaa gcatctaagt gtgaagccca cctcaagatg agatttccca tttctttaag
     1261 aaagtaagat ccctgagaga tgatcaggta gataggtcag gagtggaagt acagtgatgt
     1321 atggagcgga ctgatactaa tcgatcgagg actta
//
LOCUS       DASHHB010000167         1321 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.180, whole genome shotgun sequence.
ACCESSION   DASHHB010000167 DASHHB010000000
VERSION     DASHHB010000167.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1321)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1321)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1321
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.180"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(372..445)
                     /locus_tag="U2K71_002920"
     tRNA            complement(372..445)
                     /locus_tag="U2K71_002920"
                     /product="tRNA-Ile"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.7"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(409..411),aa:Ile,seq:cat)
     gene            complement(1000..1314)
                     /locus_tag="U2K71_002921"
     CDS             complement(1000..1314)
                     /locus_tag="U2K71_002921"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331484.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316913.1"
                     /translation="MDFYSNFILIIAILLLLNIWFFDKSRNAGIGFRTKRSTSSEKKW
                     VYSQTIFYGGVISISLLSSTLYSFNVIDVSMSNFISIIGILISAIITQLLLVFEEKSK
                     NN"
BASE COUNT          488 a          209 c          195 g          429 t
ORIGIN      
        1 actaattaac taccactcca attataagtc tttttcccat tattttttac agttatatgg
       61 tatgcttttt aatggcttca aatataaaag agtttaaagc gtaacacact tatggggaag
      121 tggtttttgg ggaacgcttt aaactcttct ttattattat ctcacaatat aacccaaatg
      181 tctttccatt taaaaatcaa agtaaaactt ttcaaatata cagaagtata actatgtgaa
      241 acatcctttc attaatccat aaaaggatac ataaaaaagc cactcatttg agtggcaatg
      301 aagaaaagct ttagcttgta taatactctt caaaaaattc taacacagaa cgattcaatt
      361 ggctacatta atgtaccctg taggactcga acctacgacc ggacggttat gagccgtctg
      421 ctctgaccaa ctgagctaag ggtagtggtt gttgccacat aaagccataa acaatcaacc
      481 agtagaatgc gtggcaacaa acctgttatc gcatatcttg gagtgtgact atttatgagt
      541 gatagtgaag atatgcgtct ccatatatgt atggttgaga cagataaaca tgtatgaaaa
      601 acgttgttaa atcaacgttt ttttattttg gtcaaggaga gaaaagtaga gaaatacaaa
      661 attttttgca ttgattttgc attcaaaaaa acattcaaac atttagaaat ccccaatacg
      721 tattcaggaa aatctatttt aaggtttaca aaaagaactt ttgttcgtac actctttttg
      781 aggtgaactt ttatgctgat ggaaggaaaa acacaattat ggtttaagca tgttccttca
      841 aataagtttt ttaattataa actctagaga aattaattca tcctaacata aaataattgt
      901 tcactcaaaa gtttttctag aacaattaaa acagcaggct tcacattcgc tatacatttg
      961 taagaagtaa taattcctgt actaaaaggc ataattgctt taattatttt ttgacttctc
     1021 ttcaaacaca agaagtagtt gtgtaataat tgcagaaata aggattccaa taatagatat
     1081 aaaatttgac atggaaacgt ctataacatt aaaagaataa agtgttgaag aaagtaaact
     1141 gattgaaata acccctccgt aaaaaatagt ctgtgaatat acccactttt tttcagaaga
     1201 cgtacttcgt ttggttctaa aacctatccc tgcatttcta ctcttatcaa aaaaccaaat
     1261 atttagaagt aataatattg ctattattaa gataaaatta ctgtaaaaat ccaaaagcta
     1321 t
//
LOCUS       DASHHB010000168         1315 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.181, whole genome shotgun sequence.
ACCESSION   DASHHB010000168 DASHHB010000000
VERSION     DASHHB010000168.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1315)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1315)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1315
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.181"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            98..>1315
                     /locus_tag="U2K71_002922"
     CDS             98..>1315
                     /locus_tag="U2K71_002922"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM7316914.1"
                     /translation="MSYTHLIKETLDILDLSVTFNENCLTKEKYKGQICHIYRGNLIY
                     TAQECIHCKHQIASDIVRWGTTTVRLLMNDVSEYRTYLELKKQRFKCKACQRTFVADT
                     SVAKKHCFISEKVRWSVVTRLKKNTSMTEIAAQKNLSVSSVYRIMKRFYRPLNPFRTP
                     LPKVLCFDEFKSVRGVSGAMSFIMMDGQTQRLLDIVENRQLPFLKRYFSHFSREIREA
                     VEWIVIDMYAPYVSLVKKLFPKAQLIIDRFHIVQHIGRTFRNHRIKETNQLLKSKEQK
                     HYQLGKQLKRYWKLLQKDERKLDYTRRLWRPGFKAHLTETDIVDRLLKGSPALRVGYQ
                     LYQDFLYAVKERDYVSFEELLTNNIMLPEGYQTTLRTFQKFLPQIKNALQQSYSNGPL
                     ECLNNHIKVLKRNA"
BASE COUNT          430 a          271 c          239 g          375 t
ORIGIN      
        1 acaccagcat cttttttatt ttccaaaaca tatagaaaag acaccttgaa atcctttaga
       61 attaatttgg cgaaccaacc caaaaggagg aatcaagatg tcttacactc accttatcaa
      121 agaaacatta gatattctag acttatcagt tacttttaac gaaaattgtc tcacaaaaga
      181 aaaatacaaa gggcaaatct gtcatattta tcgtggaaac ttgatttata cagcccaaga
      241 atgcatccac tgtaaacacc agattgcttc agatattgtg cgttggggta caacaaccgt
      301 ccggttgtta atgaacgatg tttctgaata ccgtacctac ctcgaattga aaaagcaacg
      361 cttcaaatgc aaagcttgtc agcggacctt tgtcgcagat acttctgttg cgaaaaaaca
      421 ttgtttcatc agtgagaaag tccgctggtc tgtcgtgact cgtctgaaaa aaaatacctc
      481 aatgacagag atcgccgctc agaaaaatct atcggtctct tctgtttacc gtattatgaa
      541 gcgattttat cgtccgttga atccttttcg gacaccgtta cctaaggtac tttgttttga
      601 tgaattcaaa tcagttcggg gtgtttcagg tgctatgagt ttcatcatga tggatggaca
      661 aacacagcgc ttattggaca ttgtcgagaa ccgacaactg ccttttttaa aacgttattt
      721 tagtcatttt tcacgagaaa ttcgtgaagc ggtcgaatgg atcgtgattg atatgtacgc
      781 tccttatgta tcgctcgtta aaaagctgtt tccaaaagca cagctgatta ttgaccggtt
      841 tcacatcgtc caacatattg gtcgtacctt tcgcaatcat cgaatcaaag aaacgaacca
      901 actattaaaa agcaaagaac aaaaacacta tcaattaggt aagcaattaa aaagatactg
      961 gaaacttctt caaaaagatg aacgcaagct tgattatact cgacgccttt ggcgacctgg
     1021 cttcaaagct catttaacag agaccgatat tgtggatcgc ttactgaaag ggagccctgc
     1081 tttgcgtgta ggctatcagt tatatcaaga ctttctttac gctgtaaagg aacgagacta
     1141 cgtatcgttt gaagaactac ttaccaataa tatcatgctt cccgaaggct atcagacgac
     1201 cttgagaacg ttccagaaat ttctgccaca aatcaaaaat gctttacaac aaagttattc
     1261 aaatggccca cttgaatgtc tgaacaacca tatcaaagtg ttaaaacgaa atgca
//
LOCUS       DASHHB010000169         1313 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.182, whole genome shotgun sequence.
ACCESSION   DASHHB010000169 DASHHB010000000
VERSION     DASHHB010000169.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1313)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1313)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1313
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.182"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            200..742
                     /locus_tag="U2K71_002923"
     CDS             200..742
                     /locus_tag="U2K71_002923"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002298088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316915.1"
                     /translation="MLLKVAIKCYCVKNKGGIIYEKIVPRGACLLLALCLSLSAVGCQ
                     KSNENNKIKEGQSISSEEEGMSINANGKNETFKPSDYTLEAKKEYVYEYLGLKFKLSD
                     KFRNYIADKKIAMLDDQSPIDKELKYAILTFEKMTEEQKNAVIEKMGDGYKNWQNELE
                     RIGTIGIFEKNTSEEKNLKL"
     gene            898..1272
                     /locus_tag="U2K71_002924"
     CDS             898..1272
                     /locus_tag="U2K71_002924"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA family protein disulfide reductase"
                     /protein_id="HEM7316916.1"
                     /translation="MLSEKTDLENTEAFNKESVKDLRKLPTKDINGKDFTSKDFEKYD
                     LTMVNVFATWCTACVKEIPDLVEVQNEMKSKGVNIVGVVTDPVDDNGENKEAIEKSKL
                     IHEKTKASYPFLMPDVEWQHFF"
BASE COUNT          532 a          148 c          256 g          377 t
ORIGIN      
        1 attgatgaag cagatcccaa agggctgctt catcaatttt ttcgtaagtg tccgtacact
       61 tactgtgctt gctcatatat taatcgatca tatactaatc gtgtaagaac aactttaagc
      121 ataatactta tattgttata ccatagtttg gaaaaataaa aaattatatt tcatcaacgt
      181 ttcataaaag gtgtgtagaa tgttattgaa agtagctata aaatgctatt gtgtaaaaaa
      241 taaaggagga ataatttatg aaaaaatagt accaagagga gcatgcctat tacttgcatt
      301 atgtttgagc ttaagcgcag ttgggtgtca aaaaagtaat gaaaacaata aaataaaaga
      361 aggacaatcc ataagttctg aagaggaagg catgtcaatt aatgcaaatg gcaaaaatga
      421 aacttttaag ccatcagatt atacattgga ggcaaagaaa gaatatgttt atgaatattt
      481 agggcttaaa tttaaactct cagacaaatt tagaaattat atagctgata aaaagatagc
      541 gatgttagat gatcaaagtc ctatagataa agaactaaag tatgctattt tgacatttga
      601 gaaaatgaca gaagaacaaa agaatgcagt tatagaaaag atgggcgatg gatataaaaa
      661 ttggcaaaat gagcttgaaa gaattgggac tattggtata tttgaaaaaa atacatcaga
      721 agaaaaaaat ctaaaattat aaaatgtgat actcatacta aaataggagt ttcaagtgat
      781 ggaaaatacg attgctattt aagcacaaat agcggagctg aaagtaatct tctggatgaa
      841 ttaaaaagaa ctgagattca aattatagat aaaaaagaac gtcctaaaaa tggttttgtg
      901 ctatcagaaa aaactgattt agaaaataca gaagcattta acaaagaatc ggttaaagat
      961 ttacgtaaac taccaacaaa ggacataaat ggcaaggatt ttacaagtaa agattttgag
     1021 aagtatgatc ttactatggt aaatgtattt gcaacttggt gtacagcttg tgtaaaggaa
     1081 attcctgatt tggttgaggt tcagaatgaa atgaaaagca agggtgtaaa tatcgttgga
     1141 gttgttacag atcctgttga tgataacggt gaaaacaagg aagcaattga aaagtcaaag
     1201 ttaatacatg aaaagacaaa ggcttcatat cctttcttaa tgccagatgt ggaatggcaa
     1261 cacttttttt gacaaaaatt ataaggtgtt gtgaatgctg cggtgttatg gct
//
LOCUS       DASHHB010000170         1294 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.183, whole genome shotgun sequence.
ACCESSION   DASHHB010000170 DASHHB010000000
VERSION     DASHHB010000170.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1294)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1294)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1294
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.183"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1244
                     /locus_tag="U2K71_002925"
     CDS             <1..1244
                     /locus_tag="U2K71_002925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002303202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS66 family transposase"
                     /protein_id="HEM7316917.1"
                     /translation="ITEKEHLLPLEEQFCEHCGSQMKDIGRTKVREEIRFHQAMLDCL
                     THYQHTYCCKSCEKEGLSSFKKAIVPKPLISNSLGSNSLVAETIRMKFGQKVPAYRQE
                     NYWKQTHGLDISRDNITNWHIKAVQNALDPLGERLRVYLNQEEILHGDETSYRVIESA
                     KTDTYYWQFCTGKDSQHPIVYYHHDESRAGDVPKTFLKEFTGYLHCDGYSGYNAVESV
                     RLVYCFAHVRRKFFEAIPKGKKNTDIPAAQAVKQLDKWFVLEKKWKDFSPEKRLSCRQ
                     QELRPLFIAFYEWMATIDPVAKSKLDAAVQYACKLRSGFEPIFEDGRLELTNNRAERN
                     IKELVIGRKNWLHSTSLEGARTSGIILSVYKTAELNGLNPVKYLEFLFDKIPNLPVLS
                     AETLDQLLPWNKDVQQHFSRN"
BASE COUNT          423 a          233 c          277 g          361 t
ORIGIN      
        1 ctataacaga gaaagaacat ctccttcctt tggaagaaca attttgtgaa cattgcggtt
       61 cacagatgaa agatatcgga cggacaaaag tacgagaaga aattcgtttc catcaggcta
      121 tgttggattg tctcacccat tatcaacata cctattgttg taaaagctgc gaaaaagaag
      181 gactttcttc ttttaaaaaa gcgatcgttc ctaaaccttt gatttctaat agcttaggtt
      241 ccaatagttt agtggcggaa acgatccgga tgaaatttgg tcagaaagtt cctgcctatc
      301 ggcaagagaa ctattggaaa caaacacatg ggttggatat ttcgagagat aatatcacca
      361 attggcatat taaagcagtg caaaacgcgt tagacccttt aggagaacga ttaagagtct
      421 atcttaatca agaagagatt ctacacgggg atgaaacaag ttatcgtgtg attgaaagtg
      481 ccaaaacaga tacctattac tggcagtttt gtacaggaaa agacagtcag catcccatcg
      541 tttattatca ccacgacgaa agtcgtgctg gagatgttcc gaaaactttt ctcaaggagt
      601 ttacagggta tttacactgt gacggctata gcgggtataa tgccgtggaa agtgtacgac
      661 ttgtctattg tttcgcgcat gttcggcgga aatttttcga agcaattcca aaaggaaaga
      721 aaaacacgga tattcccgca gctcaagcag tcaaacaatt ggataaatgg tttgtcctag
      781 aaaagaaatg gaaagacttt tctcctgaga agcgattaag ctgtcggcaa caagaactgc
      841 gtccattatt tattgctttt tatgagtgga tggcaacgat agatcctgtt gcaaaatcca
      901 agttggatgc agccgttcaa tatgcgtgta aactacgaag tggttttgaa cctatttttg
      961 aagatggtcg tttagaactc acaaataatc gtgcggagcg aaatatcaaa gagctggtga
     1021 taggacggaa aaattggttg cattccacaa gtcttgaagg agcacgaact tcaggaatca
     1081 ttctgagtgt atataaaaca gcagaactta acggattgaa tcctgtaaag tatctagagt
     1141 ttctatttga taagatacca aatcttcccg tactttcagc agaaacttta gatcaactgt
     1201 tgccttggaa caaggatgta caacaacatt tttctcgtaa ctaaaaacgc caaacattcc
     1261 cgattctatt ctataggtcg ggttatttga cgta
//
LOCUS       DASHHB010000171         1292 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.184, whole genome shotgun sequence.
ACCESSION   DASHHB010000171 DASHHB010000000
VERSION     DASHHB010000171.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1292)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1292)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1292
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.184"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            225..1100
                     /locus_tag="U2K71_002926"
                     /note="aph(2'')-Ia"
     CDS             225..1100
                     /locus_tag="U2K71_002926"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HEM7316918.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
BASE COUNT          550 a          105 c          208 g          429 t
ORIGIN      
        1 caagatatat taaattgatt tttgaatttt tgaaaaaaga aagaaatgct aatgcagtta
       61 ttttagaccc tcataaaaat aatccaagag caataagggc ataccaaaaa tctggtttta
      121 gaattattga agatttgcca gaacatgaat tacacgaggg caaaaaagaa gattgttatt
      181 taatggaata tagatatgat gataatgcca caaatgttaa ggcaatgaaa tatttaattg
      241 agcattactt tgataatttc aaagtagata gtattgaaat aatcggtagt ggttatgata
      301 gtgtggcata tttagttaat aatgaataca tttttaaaac aaaatttagt actaataaga
      361 aaaaaggtta tgcaaaagaa aaagcaatat ataatttttt aaatacaaat ttagaaacta
      421 atgtaaaaat tcctaatatt gaatattcgt atattagtga tgaattatct atactaggtt
      481 ataaagaaat taaaggaact tttttaacac cagaaattta ttctactatg tcagaagaag
      541 aacaaaattt gttaaaacga gatattgcca gttttttaag acaaatgcac ggtttagatt
      601 atacagatat tagtgaatgt actattgata ataaacaaaa tgtattagaa gagtatatat
      661 tgttgcgtga aactatttat aatgatttaa ctgatataga aaaagattat atagaaagtt
      721 ttatggaaag actaaatgca acaacagttt ttgagggtaa aaagtgttta tgccataatg
      781 attttagttg taatcatcta ttgttagatg gcaataatag attaactgga ataattgatt
      841 ttggagattc tggaattata gatgaatatt gtgattttat atacttactt gaagatagtg
      901 aagaagaaat aggaacaaat tttggagaag atatattaag aatgtatgga aatatagata
      961 ttgagaaagc aaaagaatat caagatatag ttgaagaata ttatcctatt gaaactattg
     1021 tttatggaat taaaaatatt aaacaggaat ttatcgaaaa tggtagaaaa gaaatttata
     1081 aaaggactta taaagattga ttatataata tatgaaaagc tattataaaa gacattagta
     1141 ttaaatagtt taaaaaaatg aaaaataata aaggaagtga gtcaattcca gactcctgtg
     1201 taaaatgcta tacaatgttt ttaccatttc tacttatcaa aattgatgta ttttcttgaa
     1261 gaataaatcc attcatcatg taggtccata ag
//
LOCUS       DASHHB010000172         1234 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.185, whole genome shotgun sequence.
ACCESSION   DASHHB010000172 DASHHB010000000
VERSION     DASHHB010000172.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1234)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1234)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1234
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.185"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            433..966
                     /locus_tag="U2K71_002927"
     CDS             433..966
                     /locus_tag="U2K71_002927"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease"
                     /protein_id="HEM7316919.1"
                     /translation="MNFVAMDFETANHQPYSACSLALVMVKNSQIVDEFYTLIQPETP
                     FFWRNVQIHGIHQEDVRNAPKFPEVWQTIQPYFQKNRLVVAHNAAFDTKVLAGCLDYY
                     HLEQPNYLSLCTVKSSRRLFPEFPNHRLNTVCENLNIQLDHHHDALEDSRACAEILLY
                     QEKKFGTDPLKKLVTIK"
BASE COUNT          391 a          227 c          230 g          386 t
ORIGIN      
        1 tatttttcgt ttggttggac ttattaccgc agatgcaagc ttttgaacac cgtttattga
       61 gaagttgtga aatcagcgtc ctgacctgag tattagagaa acaaatggca aaatagttcc
      121 tcatattttg ctattttttg agctaatacc gagggtcagc tgattgaaca ccgtttagtg
      181 gagaagttgt gaaaaagtcg cctactttcg agtcgtaaga aatttttttg aaaatggttc
      241 ctcacaactt tgcataaatt tgacttaata ccgagaagtc atgatcccca tctaactcca
      301 aatcacaagg aatgagttca aaaactagct tttctcaaca aatattacat aaaaaattct
      361 atgttttttg ttaaatttat ttgctttttt caaaagtatg ttacagtaat ttcccaagga
      421 ggttttacag aaatgaattt tgttgccatg gattttgaaa ctgccaatca tcagccttat
      481 agtgcttgtt ctcttgcgct agtcatggta aaaaatagcc agatcgttga tgaattctat
      541 acattgatac agcctgagac tccttttttc tggcgaaatg tccaaattca cggaatccat
      601 caagaagacg tacgcaatgc gccgaaattc ccagaagtct ggcaaactat ccaaccatat
      661 tttcaaaaaa atcgtttagt cgttgcacat aatgctgctt ttgatacaaa ggtcttagcg
      721 ggatgcttag attattatca tcttgaacag cctaattatc tttctttatg tacagtgaaa
      781 agtagccgtc gcctgtttcc tgaatttcca aatcatcgat tgaacactgt gtgcgaaaat
      841 ttgaacatcc aattagatca tcaccatgat gcattggaag atagtcgtgc atgtgcggaa
      901 atcctgcttt atcaagaaaa aaaattcggg acagatccat tgaaaaaact tgtgactata
      961 aagtaaaaga atacttatga aacacacaaa gaggtcgtga gcctgccgtt tcactccgag
     1021 tcacaaggag caatttcaaa aaatagcttc tcatgtttat taaatattgt gactggtgac
     1081 cgaggagctg gcaggagaac accgtttatt ggagaggttg tgaaaaagct gttctgcatc
     1141 aagtaataag aacagccaaa caaaaatagc ttctcatatt tttcatttgg ttggacttat
     1201 taccgcagat gcaagctttt ggacaccgtt tacc
//
LOCUS       DASHHB010000173         1215 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.186, whole genome shotgun sequence.
ACCESSION   DASHHB010000173 DASHHB010000000
VERSION     DASHHB010000173.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1215)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1215)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1215
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.186"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>1215
                     /locus_tag="U2K71_002928"
     CDS             <1..>1215
                     /locus_tag="U2K71_002928"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_086557923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
                     /protein_id="HEM7316920.1"
                     /translation="IYFEENCLTKEKYKGQICLIYKGTLLYKPEECSHCLCVVPSRII
                     RWGTTTVRLLLNDVSEYRTYLELKKQRFKCKSCQRTFVADTSVAEKHCFISQKVRWSV
                     IARLKENTSMTEIARQKNISTSSVYRVMKRFYRPLNPFKQTLPKVLCFDEFKSVRSVA
                     SAMSFIMMDGQSHALLDIVENRRLPYLERYFSRFSLATREAVQLIVIDMYAPYVSLVK
                     KLFPNAQLIIDRFHIVQHIGRTFLNHRVKETTVRLKGNSHSQRGLGKKLKRYWKLLQK
                     EEAKLNYEKRVWRASFKDYLTESEIADRLLAACPNLRQGYQLYQDILYAVKKRDQSLF
                     EDCLTRDISGLPETYTTTLRTFKKFLPQIQNALHYSYSNGPLECLNNHIKVLKRNAYG
                     FRNFYNFKLRIMI"
BASE COUNT          372 a          241 c          241 g          361 t
ORIGIN      
        1 atctatttcg aggaaaattg tttaactaaa gaaaaataca aagggcaaat ttgcttgatt
       61 tacaaaggaa ctcttctgta caagccagaa gagtgttccc attgtctttg tgtcgtcccc
      121 tctaggatca ttcgttgggg gacgacaacc gttcggttgt tactgaatga tgtgtctgag
      181 tatcgcactt atctggagct gaagaaacaa cgatttaaat gtaagtcttg ccaaagaaca
      241 ttcgtagcgg atacctctgt tgctgaaaaa cactgtttca ttagtcaaaa agttcgttgg
      301 tctgtcattg ctcgcttaaa agagaatact tccatgacgg aaatcgctcg tcagaaaaat
      361 atttccactt cttcggtcta tcgtgtcatg aagcgattct accgcccatt gaatcctttc
      421 aaacagactt tgccaaaggt cttatgcttt gatgaattca aatcggttcg aagtgttgcc
      481 agtgcaatga gttttatcat gatggacgga caaagccatg cattattgga tattgtcgaa
      541 aatcgacgct tgccttattt agaacgctat ttttctcggt tttcattagc gactcgtgaa
      601 gccgttcaat tgattgttat tgatatgtat gcgccttatg tttcgttggt gaaaaagctt
      661 ttcccgaatg ctcagttgat catcgatcgt ttccacatcg tccaacatat tggcagaact
      721 ttcttgaatc atcgagtgaa agaaaccacg gtacgactta aaggaaattc ccactctcaa
      781 cgagggttag ggaaaaaatt gaagcgctat tggaaactcc tacaaaaaga agaagcaaag
      841 ttaaactatg aaaaacgggt ttggcgtgcc agttttaaag attatttgac tgaaagtgag
      901 attgccgatc gactgttagc tgcttgtccc aacttacgcc aaggctatca gttataccaa
      961 gacattcttt atgcggtaaa gaaaagagat cagtcactat ttgaggattg tttaactaga
     1021 gacattagcg gtctgccaga aacctataca accactttac gaacgtttaa aaagtttctg
     1081 ccacagatcc aaaatgcgct tcactacagc tattcaaacg gaccattgga atgcttaaac
     1141 aaccacatca aagtattaaa acgcaatgcg tacggctttc gcaactttta caatttcaaa
     1201 ctacgaatta tgatt
//
LOCUS       DASHHB010000174         1197 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.187, whole genome shotgun sequence.
ACCESSION   DASHHB010000174 DASHHB010000000
VERSION     DASHHB010000174.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1197)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1197)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1197
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.187"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(105..629)
                     /locus_tag="U2K71_002929"
     CDS             complement(105..629)
                     /locus_tag="U2K71_002929"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008268881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HEM7316921.1"
                     /translation="MSEEVGIILREAVPGDAKDILLMMGQVNKETEFLVLDEAELLLP
                     PETLEEELDYIYESNNNLLLLAIYEGTIIGTASVKADSQFRLSHVGEVGISILQEYWG
                     MGLGTLMLEEIISWAKEMGVLFRLELDVQVRNERAVHLYRKMGFQIEAVMPRGARTDL
                     GEFLDVYKMSYLIE"
     gene            complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U2K71_002930"
     CDS             complement(622..1095)
                     /gene="tsaE"
                     /locus_tag="U2K71_002930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HEM7316922.1"
                     /translation="MIELSGLTMTEQFAEAIGHSAMPGDNLILTGDLGAGKTTLTKGI
                     AQGLGITQMIKSPTYTIIREYSQGRIPLYHMDIYRVAASGADLGLDEYFEGDGLSVVE
                     WGNLLEEALPEDYLELILEKSDTDLEKRYVKLISYGEQSETFKQRILEKWAENHE"
BASE COUNT          313 a          301 c          173 g          410 t
ORIGIN      
        1 tattcaaaaa aaatggactg cgacacgact tttgtcacag tcccttcgtg tgtttttctg
       61 ccttctcaaa aggatgacag tttctcactc ttttcctatt tcttctattc aatcaaataa
      121 ctcattttat atacatctaa aaattcacct agatctgttc ttgcccctct aggcatgacc
      181 gcttcgattt ggaagcccat ttttcggtaa aggtgtacgg ctctttcatt acgaacttga
      241 acgtcaagct ccaatcgaaa aagtacaccc atttccttcg cccaactgat gatttcttct
      301 agcataagag tgcctagtcc cattccccaa tattcctgca gaatactgat accgacttct
      361 cccacgtgag acaaacgaaa ttgactgtct gccttgacag atgctgttcc aataatcgtc
      421 ccttcataga ttgcaagcag caataaatta ttattgcttt cgtatatata atccaactct
      481 tcttctaagg tttctggtgg taacaggagt tctgcttcat ccaaaactaa aaattctgtc
      541 tccttattaa cttgtcccat catcaaaaga atgtcttttg catctcctgg cactgcttcc
      601 cgcaagatga tcccaacttc ttcactcatg attttcagcc catttttcta gaattcgctg
      661 tttgaaggtt tcactttgct ccccgtatga gattagtttc acataccgct tttctaaatc
      721 tgtatcactt ttttctaaaa tcagttccaa ataatcctcc ggcagtgctt cttctaatag
      781 atttccccat tcaacaacgg aaagcccatc accttcaaaa tattcatcta accctaaatc
      841 tgctccgcta gctgctaccc gataaatatc catatgatac aacggaatcc gaccttgact
      901 atattctcta ataatcgtgt atgtcggact tttgatcatt tgcgttatcc ctaatccttg
      961 ggcaattcct ttcgtcaacg ttgttttacc cgcacctaga tcaccagtca atatcagatt
     1021 gtcacctggc attgcggaat gaccaattgc ctctgcgaat tgttctgtca ttgttaatcc
     1081 tgatagttcg atcatttttc atcttctccc gctcttcttt ctttttgcca gtatatcgcg
     1141 taatgaagtt tttttaaaga aaaaatgaac tagtcaagaa cagcagctgc tgttttt
//
LOCUS       DASHHB010000175         1193 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.188, whole genome shotgun sequence.
ACCESSION   DASHHB010000175 DASHHB010000000
VERSION     DASHHB010000175.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1193)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1193)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1193
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.188"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1150
                     /locus_tag="U2K71_002931"
     CDS             join(<1..264,264..1150)
                     /locus_tag="U2K71_002931"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEM7316923.1"
                     /translation="RRTYSKEFKQQIVDLYLAGKPRAEIIREYELTPSSFDKWMKQAQ
                     STGSFKERDNLTPEQAELIALRKKNKQLEMENDILKQAALIFGPKRQVIDANKHKYSI
                     SAMCKILNISRQTYYYQAKPIENESDLEEIVQEEFIRNRKAYGTRKLKKCLAKRGLQL
                     SRRRIGRIMKRRGLTSTYTIAHFKGQRTACNEAKTANVLDRTFTQEQPLEAIVTDLTY
                     VRVGKKWHYICLILDLFNREIIGYSCGEKKDASLVKEAFGRIPYSLTDVKLFHTDRGK
                     EFDNQTIHEILNGFGITRSLSRKGCPYDNAVVESTYKSVKVEFVHQYQFETLAQLRLE
                     LFDYVHWWNYLRLHGTLAYETPIQIRQQRLAKRILDNERGSDTSGEAA"
BASE COUNT          398 a          227 c          264 g          304 t
ORIGIN      
        1 aacgaagaac ctattcaaaa gaattcaaac aacaaatcgt cgatctctat ctcgctggta
       61 agcctcgcgc agaaattatt cgagagtatg agcttacgcc ttcttctttc gataaatgga
      121 tgaagcaagc acaatcaacg ggctcattca aagaaagaga caacttaaca ccagaacaag
      181 cagaattgat cgcactaaga aagaaaaata agcaactcga aatggagaat gatattttaa
      241 agcaagcggc gctgatattc ggacgaaaag acaagtaatt gatgccaaca agcataaata
      301 ttccatatca gcgatgtgca aaattctaaa tatttctcgt caaacctact attatcaagc
      361 gaaaccgatc gaaaatgagt ccgacttaga agagatcgtt caggaagagt ttattcgaaa
      421 ccgaaaggct tacggtaccc gaaaattgaa gaagtgttta gcaaagcgtg ggcttcaact
      481 cagtcggcgc cgaatcggtc gaatcatgaa acgccgcgga ttgacatcta cctatacgat
      541 cgctcatttt aaagggcaac gaacagcttg taatgaagcg aaaacagcga atgtattaga
      601 tcggaccttt acacaagaac agccattgga agccatcgtt acggatctta cttatgttcg
      661 cgtggggaaa aagtggcatt atatctgctt aatacttgat ttgtttaatc gagaaattat
      721 tggttattcc tgtggtgaga agaaagatgc ctcattggta aaagaagcct ttggacggat
      781 accgtattct ttaacagacg tcaagctttt tcatacagac cggggaaagg aatttgataa
      841 ccaaaccatt catgagattc tgaatggttt tggaattact cgttcattga gtaggaaggg
      901 ttgtccgtat gataatgccg ttgtggaatc aacctataaa tctgtcaaag tagaattcgt
      961 gcatcaatac caatttgaga cactggcaca gctacgtcta gaattgtttg attatgtgca
     1021 ttggtggaac tatctacgct tacatggcac gttggcgtat gaaacaccga tccaaattcg
     1081 acaacagaga ttggcgaagc gaatccttga taatgagcgc ggatctgata cctctggaga
     1141 ggcagcgtaa ttgaatgatt gtgcttctgc cggagaaaat cagatccgag gat
//
LOCUS       DASHHB010000176         1192 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.189, whole genome shotgun sequence.
ACCESSION   DASHHB010000176 DASHHB010000000
VERSION     DASHHB010000176.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1192)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1192)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1192
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.189"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1076
                     /locus_tag="U2K71_002932"
     CDS             <1..1076
                     /locus_tag="U2K71_002932"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014748673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEM7316924.1"
                     /translation="VIGMESTSMYSFHPSMFFHEDEKLKKLNTLVTIENPFRVKQFSR
                     MFDENKTDRNDALRIADFLRIQRFTTSPIKEEKYMALQRLTRTRYQLIKQLIRTKQHF
                     LENLTYKCNTLAREMRDESTSLFSATLISLMTEDFTLDELAELPLEAFCDLLQEKGKG
                     RFKQPEKIAKAIQRAITMSYRLGALAQESINVVLSVLVREIRALEKNIKELDKAIEQV
                     IVVIPEYQCLTSIPGVGKVYAAGLIAEIGQIERFEDQTKLAKYAGLSWKVNQSGNYQS
                     QNTPLTKQGNRYFRYYLVEAANSVKNYLPEYKAFYQSKYKEVPKHQHKRALVLTARKF
                     TRLVDTLLRKHQLYTPPRSVIEK"
BASE COUNT          418 a          234 c          210 g          330 t
ORIGIN      
        1 ttgtgattgg catggagtca acgtccatgt acagcttcca tccttccatg ttttttcatg
       61 aagatgagaa actaaagaag ctcaacacac ttgtaacgat tgaaaatcct tttcgggtga
      121 aacaatttag tcgcatgttt gatgagaata aaactgatcg aaacgatgca ttaagaatcg
      181 ctgatttctt gcgtattcaa cgattcacaa cttcccctat caaagaagag aaatacatgg
      241 ccttacagcg tttaacacgt actcgctatc aacttattaa gcaattgatt cgtacaaagc
      301 aacattttct tgaaaatcta acgtacaaat gtaatacgct tgctcgagaa atgcgagacg
      361 aatcgacgag tctttttagt gcaaccctca tttcactcat gacggaagat ttcacattgg
      421 atgaacttgc tgagcttcct ttagaagctt tctgtgattt acttcaagaa aaaggaaaag
      481 gccgtttcaa gcaaccagaa aaaatcgcta aagcaattca acgtgcaatt accatgagtt
      541 accgcctagg tgctcttgca caagaatcaa ttaatgttgt tttaagtgtt ctcgtacgag
      601 aaatacgagc acttgaaaaa aatatcaaag aattagataa agccatcgaa caagtaattg
      661 ttgtcattcc agaatatcaa tgcttaacca gtattccagg cgttggtaaa gtctatgctg
      721 ccggtttaat cgctgaaatt ggtcaaatcg aaagatttga agatcagaca aaattggcaa
      781 aatacgctgg attaagttgg aaagtgaacc aatctggaaa ctatcagtcg caaaatacac
      841 ctctgacaaa acaaggaaat cgatatttta gatactactt agttgaagct gccaactctg
      901 taaaaaatta tcttcctgaa tacaaagctt tttatcaaag taaatataag gaggttccaa
      961 agcatcaaca caaacgtgca ctcgtcctaa ccgcaagaaa atttacgcgt ctggtggata
     1021 cgctactacg taaacaccaa ctctatacgc caccaaggag tgtgatagag aaataacaat
     1081 ccgttattga cgcaaatcct tgaatcaacc cgaaaaaaat ttgattttga tcgggtctag
     1141 tttcgtgcgc ttcaaaacat attactcaat aaagaaaatc ttaaatttca tc
//
LOCUS       DASHHB010000177         1134 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.190, whole genome shotgun sequence.
ACCESSION   DASHHB010000177 DASHHB010000000
VERSION     DASHHB010000177.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1134)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1134
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.190"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            55..>1134
                     /locus_tag="U2K71_002933"
     CDS             join(55..331,331..>1134)
                     /locus_tag="U2K71_002933"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611768.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS3 family transposase"
                     /protein_id="HEM7316925.1"
                     /translation="MSKRTRRTFSQEFKQQIVNLYLAGKPRVEIIREYELTASAFDKW
                     VKQSKTSGSFKEKDNLTPEQKELLELRKRNQQLEMENDILKQAALIFGPKRQVIDANK
                     HLYPISAMCRILGLSRQSYYYQSKPKKDESELEEVVAEEFIRSRKAYGSRKIKKALSK
                     RGIQISRRKISRIMKNRGLKSSYTVAYFKVHHSTCNEAKTTNVLNRKFLRDNPLEAIV
                     TDLTYVRVGKKWNYVCFILDLFNREILGYSCGEHKDAVLVKKAFSRIKQPLTEVEIFH
                     TDRGKEFDNQAIDELLTTFDINRSLSHKGCPFDNAVAESTYKSLKVEFVYQYTFETLQ
                     QLDLELFDYVNWWNHLRLHGTLGYET"
BASE COUNT          425 a          192 c          217 g          300 t
ORIGIN      
        1 gacagaaaaa ttaaggtgtg tagactagaa gaaaacatac caggaggaat ttttatgtct
       61 aagagaacac gaagaacttt ttcacaagaa ttcaagcaac aaatcgtcaa tctttactta
      121 gctggaaagc cacgtgtaga aatcattcga gaatatgaac taacggcttc agcatttgac
      181 aaatgggtaa agcaatctaa aacgagtggt tcattcaaag aaaaagataa tcttacgcct
      241 gaacaaaaag aattgttaga actacgtaaa agaaaccagc aattagaaat ggaaaatgat
      301 attttaaagc aagcagcgct gatattcgga cgaagagaca agtaatcgat gcgaataagc
      361 atctttaccc tatatcagcg atgtgcagaa tattaggtct atcacgtcag tcctattatt
      421 atcaatcaaa accaaagaaa gacgaatcag aacttgaaga agtagtcgct gaagaattta
      481 tccgcagccg aaaggcctac ggctcaagaa aaataaaaaa agccttatca aaacgaggca
      541 ttcagatcag ccgacgaaaa attagtagaa tcatgaaaaa tagaggatta aaatcgagct
      601 atactgttgc ttattttaaa gtacatcatt ctacttgcaa tgaagccaaa acgacaaacg
      661 tattgaatcg taaattctta agagacaacc cattagaagc gatcgtaaca gacttgactt
      721 atgtacgagt cgggaaaaaa tggaattatg tctgtttcat tttggatctg ttcaatcgag
      781 aaattctcgg ctattcttgt ggagaacata aagatgccgt tctagtaaaa aaagcattta
      841 gccgtatcaa acaacctctg acagaggttg agatttttca tactgatcgt ggaaaagagt
      901 ttgataacca agctattgat gaattattaa caacttttga catcaatcga tcattgagtc
      961 ataaaggctg tccttttgat aatgccgtag ctgaatcaac ttataagtcg ttgaaagtag
     1021 aatttgtcta tcaatacaca tttgaaacct tacaacaatt ggatttggag ttatttgact
     1081 atgtcaattg gtggaaccac cttcggttgc acggtacact tggctacgag acac
//
LOCUS       DASHHB010000178         1106 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.191, whole genome shotgun sequence.
ACCESSION   DASHHB010000178 DASHHB010000000
VERSION     DASHHB010000178.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1106)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1106)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1106
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.191"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..1087
                     /locus_tag="U2K71_002934"
     CDS             <1..1087
                     /locus_tag="U2K71_002934"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002309068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM7316926.1"
                     /translation="DINDDGSRKKVEKVGGDTRPEAEAALRKVLSDIDETGQYFLGTD
                     TRVKQYLDFWMEEYVKLNLKYNTYENYRFTIKNHINGYLGKKKLTDLSPALLQNFINA
                     EFKKGYSKKTMTITHSVLKNALNMAVYPWGLIKQNPMLYVKIPKYEARPTTKKDLKII
                     SLEDFDHMLEITPEGHPFYIPLNIGFYTGMRVGEVCGLTWDDVDFSNGTITVEKQMVK
                     NDGAWVYGTPKTSSSNRTIFIGQTLLAILKKHKKQQLENRMKYGKLYIDSNAVCTKED
                     GELVTPSVVKWNTRRISNALSLSFNFHSLRHTHATLLLENGAKMKEISERLGHSRISI
                     TMDTYSHVTDKMRNETVDIMENLRKNS"
BASE COUNT          403 a          192 c          214 g          297 t
ORIGIN      
        1 agatatcaat gatgatggct caagaaaaaa agtggagaaa gttggcggag acacccgacc
       61 agaggccgaa gctgctttac gaaaagtttt atcagatatt gacgaaacag gacaatactt
      121 tttaggtacg gatactcgag taaaacaata ccttgatttt tggatggagg aatacgttaa
      181 actaaatcta aaatacaata cctatgaaaa ctaccgattt accatcaaaa atcatataaa
      241 cggttattta ggaaagaaaa aacttacgga tctctcccct gctcttttac aaaatttcat
      301 caatgctgaa tttaaaaagg gttactcgaa gaaaacaatg actattactc actctgtcct
      361 taagaatgcg ctgaatatgg cggtttatcc ttggggatta atcaagcaaa atcctatgct
      421 gtatgtaaaa ataccaaaat atgaagcacg accaacgact aaaaaagatc taaaaatcat
      481 ttctcttgag gactttgatc atatgctaga aattactcct gaaggacatc ctttctatat
      541 tcctttgaat attggatttt atacgggaat gcgcgttggc gaagtttgtg gtctgacgtg
      601 ggatgatgtc gatttttcaa atggaacaat tactgtagag aaacaaatgg taaagaatga
      661 cggcgcatgg gtatatggta cgccaaagac aagcagttcc aatcgaacga tttttattgg
      721 acaaaccttg ctagcaattc tgaaaaaaca taagaaacaa caattagaaa atcgaatgaa
      781 gtatggaaag ctctacattg attcaaatgc agtatgtacg aaggaagacg gtgagctagt
      841 tacgccaagt gtagtgaaat ggaatactag gaggatatcg aatgcactct ccctctcttt
      901 taacttccat tctctcagac atactcatgc tacacttctt ctcgaaaatg gcgcaaaaat
      961 gaaagaaatc tctgaacgat tgggccacag cagaatttca attacgatgg atacttactc
     1021 gcatgtaaca gataagatga gaaatgaaac ggtcgatatc atggaaaatc ttagaaagaa
     1081 ttcttgattt ttgccaccga aaaaaa
//
LOCUS       DASHHB010000179         1063 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.192, whole genome shotgun sequence.
ACCESSION   DASHHB010000179 DASHHB010000000
VERSION     DASHHB010000179.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1063)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1063)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1063
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.192"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(37..993)
                     /locus_tag="U2K71_002935"
     CDS             complement(37..993)
                     /locus_tag="U2K71_002935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002326835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316927.1"
                     /translation="MELYTLPIIIKTEKMLKDLKQYYPLSAYEKKKLFHFLAINLLAE
                     QADSLDIASNQSLFFSDIGFYHLKGVLGEKSVIFINKTLSWLYLHDKLDQKYLRKNED
                     ETIEWLNERFIESFENQFNLLREETSQLIRRELTKIHFKLLHYTINKADEFPLDVSFF
                     RENYPAFYCYLIEYINDLTSKRKELLQDKFFLFFSYLLLLINHIPIQLISEPVKIIID
                     FSYGAAYNQFIKKNLSLYINLNTEVIEAGKPEFPDVIITNRNNLYEESASQVVVWLDP
                     PRAVDWGNLTKILLTIQEEKYKDSKIDKQIDDFPEEIIDRIE"
BASE COUNT          346 a          178 c          160 g          379 t
ORIGIN      
        1 ttagaaaaag cagtcggact ttctaaaaga agttcactat tcgatcctgt cgatgatttc
       61 ttcaggaaaa tcgtctattt gcttgtcaat tttgctgtct ttgtatttct cttcttggat
      121 agtcaacaaa atctttgtaa gattccccca gtctactgct cttggcggat cgagccaaac
      181 cactacttga gatgcacttt cttcatatag attattccta ttcgtgatta taacatccgg
      241 gaactcaggc tttcctgcct ctatcacttc cgtgttcaga ttaatgtaca agcttaaatt
      301 tttcttgata aattgattat aggcagcacc ataagaaaaa tcgatgatga tctttactgg
      361 ttcagaaatc agttgaatgg gtatgtgatt gatcaataat aataaataac tgaaaaaaag
      421 aaaaaattta tcttgaagca actctttgcg ttttgacgtt agatcattta tatactctat
      481 caaatagcaa taaaatgctg gatagttttc tctaaaaaaa gaaacatcaa gtggaaattc
      541 atcagcttta ttaatagtgt aatgcaaaag tttaaaatgt attttggtta actctcttcg
      601 gatcaattgg ctcgtctctt ctcgcaacag attaaactga ttttcaaaac tttcaatgaa
      661 tcgttcattc aaccattcga ttgtttcatc ttcatttttt ctcaagtatt tttgatccaa
      721 tttatcatga agataaagcc aagagagtgt cttattgata aaaataacgg atttttctcc
      781 taatactcct tttaagtggt aaaatcctat atccgaaaaa aatagcgact gattgctagc
      841 aatatctaaa gaatcagctt gttcagccaa caggttgatt gctaaaaagt ggaataattt
      901 ctttttttca taagcagaca gaggataata ctgttttaaa tcttttaaca ttttttctgt
      961 ttttataatt ataggtagcg tatacaattc cacaaaactt atctctgttg agttaaaaag
     1021 attatataaa aaaagccgaa tgtttttagg attccctgtc aac
//
LOCUS       DASHHB010000180         1045 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.193, whole genome shotgun sequence.
ACCESSION   DASHHB010000180 DASHHB010000000
VERSION     DASHHB010000180.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1045)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1045)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1045
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.193"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            6..980
                     /gene="bsh"
                     /locus_tag="U2K71_002936"
     CDS             6..980
                     /gene="bsh"
                     /locus_tag="U2K71_002936"
                     /EC_number="3.5.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="choloylglycine hydrolase"
                     /protein_id="HEM7316928.1"
                     /translation="MCTSITYVTSDHYFGRNFDYEISYNEVVTVTPRNYKLNFRKVND
                     LDTHYAMIGIAAGIADYPLYYDATNEKGLSMAGLNFSGYADYKEIQEGKDNVSPFEFI
                     PWILGQCSTVGEAKKLLKNINLANINYSDELPLSPLHWLLADKEKSIVIESMKDGLHI
                     YDNPVGVLTNNPSFDYQLFNLNNYRVLSSETPKNNFSNQISLNAYSRGMGGIGLPGDL
                     SSVSRFVKATFTKLNSVSGDSESESISQFFHILGSVEQQKGLCDVGDGKYEYTIYSSC
                     CNVDKGIYYYRTYEDSQITAIDMNKEDLDSHKLISYPIIEKQQIKYIN"
BASE COUNT          376 a          132 c          182 g          355 t
ORIGIN      
        1 aaactatgtg tacgtctatt acttatgtaa caagtgatca ttattttgga aggaattttg
       61 attatgaaat atcttacaat gaagtagtta ctgttactcc aagaaattat aagttgaatt
      121 ttcgaaaggt aaatgatttg gatactcatt atgcaatgat tggtattgcc gctggtatag
      181 ctgactaccc tctttattac gatgcgacaa atgaaaaagg attgagtatg gctgggctaa
      241 atttttctgg gtatgctgat tataaagaaa tacaagaagg gaaagacaat gtatctcctt
      301 ttgaatttat tccttggatt ttaggacaat gctcaacagt aggagaagct aaaaaattgt
      361 taaaaaatat caatttagca aatataaatt atagtgacga acttccttta tcccctttac
      421 attggctatt agctgataaa gaaaaatcaa ttgtcattga aagtatgaaa gatggacttc
      481 atatatatga taaccctgtg ggcgttctta ccaataatcc ttcatttgac tatcaattat
      541 ttaatttaaa caattatcgt gtcttatcga gtgaaactcc taaaaataat ttttcaaatc
      601 aaataagttt gaatgcctat agccgcggta tgggagggat aggcttgcct ggagatttat
      661 cctcagtatc tcgttttgtt aaagcgactt ttacgaagct gaattctgta tctggagatt
      721 cagagtcaga aagtattagt caatttttcc atatcttagg ttcagtagaa caacaaaaag
      781 gtttgtgtga tgtaggtgat ggaaaatatg aatatacaat ttattcttct tgttgcaatg
      841 ttgacaaagg aatctattat tatcgaacat atgaagacag tcaaattact gcaattgata
      901 tgaataaaga agacttagat agtcataagt taattagtta tccaattata gaaaaacaac
      961 aaattaaata tataaattag ttaatgtgtt gtgattgatt atttaatata gatataatac
     1021 aaaaaggcaa agatattttc aaaac
//
LOCUS       DASHHB010000181         1001 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.194, whole genome shotgun sequence.
ACCESSION   DASHHB010000181 DASHHB010000000
VERSION     DASHHB010000181.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1001)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1001)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1001
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.194"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>1001
                     /locus_tag="U2K71_002937"
     rRNA            <1..>1001
                     /locus_tag="U2K71_002937"
                     /product="23S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:12.0:RF02541"
                     /inference="COORDINATES: profile:INFERNAL:1.1.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF02541"
BASE COUNT          311 a          198 c          288 g          204 t
ORIGIN      
        1 agctatgatc cagagatttc cgaatggggg aacccaacat ctttaatagg atgttacgat
       61 tgtgtgaata catagcacat tcgaggtaga cgcagagaac tgaaacatct aagtacctgc
      121 aggaagagaa agaaaattcg attccctgag tagcggcgag cgaaacggga aaagcccaaa
      181 ccagcaagct tgcttgttgg ggttgtagga ctccaatatg gtagttcttt cagatagtcg
      241 aatgacttgg aaaagtcagt caaagagggt aaaaaccccg tagacgaaat gtggaagaca
      301 cctaggagga tcctgagtac ggcggaacac gagaaattcc gtcggaatcc gggaggacca
      361 tctcccaagg ctaaatactc cctagtgacc gatagtgaac cagtaccgtg agggaaaggt
      421 gaaaagcacc ccggaagggg agtgaaatag aacctgaaac cgtgtgccta caacaagtca
      481 aagcccgtta atgggtgatg gcgtgccttt tgtagaatga accggcgagt tacgattgca
      541 tgcgaggtta agttgaagag acggagccgc agcgaaagcg agtctgaata gggcgtttga
      601 gtatgtagtc gtagacccga aaccatgtga tctacccatg tccaggttga aggtgcggta
      661 aaacgcactg gaggaccgaa cccacgtacg ttgaaaagtg cggggatgag gtgtgggtag
      721 cggagaaatt ccaaacgaac ttggagatag ctggttctct ccgaaatagc tttagggcta
      781 gcctcggaat tgagaatgat ggaggtagag cactgtttgg actaggggcc catctcgggt
      841 taccgaattc agataaactc cgaatgccat tcattcatat ccgggagtca gactgtgagt
      901 gataagatcc atagtcgaaa gggaaacagc ccagaccacc agctaaggtc ccaaaatata
      961 tgttaagtgg aaaaggatgt ggggttgcac agacaactag g
//
LOCUS       DASHHB010000182          925 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.195, whole genome shotgun sequence.
ACCESSION   DASHHB010000182 DASHHB010000000
VERSION     DASHHB010000182.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 925)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 925)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..925
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.195"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..582)
                     /locus_tag="U2K71_002938"
     CDS             complement(<1..582)
                     /locus_tag="U2K71_002938"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316929.1"
                     /translation="MINSAYFHIKYLKKYEYEEVVLDYGLNRQSFLIYAINNGNLPSN
                     KKNIIIETYAYLEGGSKKLIKSCNIEYQLSVGEIKCVFEEDLELDLKKIFASIHDYIG
                     LIYKIIEDDKQTELNMCYFDREQDFFYYGGMGGGSIVPKINLVELDVNNYRELYVFNS
                     LDFEIPMKQMKNIDIHILPSESCQIEYSIEYFYK"
     gene            complement(822..>925)
                     /locus_tag="U2K71_002939"
     CDS             complement(822..>925)
                     /locus_tag="U2K71_002939"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM7316930.1"
                     /translation="WTCPICHTHHDRDINASINILTEGLRLHSMGLA"
BASE COUNT          286 a          162 c          122 g          355 t
ORIGIN      
        1 tttgtaaaaa tattcgattg aatattctat ttggcacgac tcacttggca gtatatgtat
       61 gtcaatattt ttcatctgtt tcataggtat ttcaaaatct aaactattaa aaacatataa
      121 ttccctgtaa ttatttacat ctaactctac taaatttatc tttggaacaa tagatcctcc
      181 acccattcca ccataataaa aaaaatcttg ttctctatca aaatagcaca tattaagctc
      241 agtttgttta tcatcctcaa taattttata aatcaaacct atataatcat gaatgctggc
      301 aaaaattttt tttaagtcaa gttctaaatc ttcttcgaaa acacatttaa tttctcctac
      361 tgataattga tactctatat tacatgactt aattagtttt ttagatcccc cctccagata
      421 ggcatatgtt tcaataatta tgttcttttt attgcttggc aaatttccgt tatttattgc
      481 ataaattaaa aatgactgtc tatttagccc ataatctaat acaacttctt catactcata
      541 tttctttaaa tatttaatat gaaaatatgc tgaattaatc aaaactcgag tgttactgtt
      601 gttatttatt gttgttctat tgtgtataaa attgtatagg ttgtcatatt ttcttggttt
      661 agtgaactac tcaccactta gctaaaccta acggtttctt acgtttgaag tgggagcttc
      721 ttgggaagag agcatacttg atagcgtatt actttactaa cagaggtgcc tcttatttac
      781 caagctatcc ccgtagttcc tacggttctt gtttatttga tctaagccaa tcccatagag
      841 tgtagcctta gaccttcggt caggatattg atactagcgt tgatatctcg gtcatgatga
      901 gtatgacaaa tagggcaagt ccatt
//
LOCUS       DASHHB010000183          890 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.196, whole genome shotgun sequence.
ACCESSION   DASHHB010000183 DASHHB010000000
VERSION     DASHHB010000183.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 890)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 890)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..890
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.196"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            114..548
                     /locus_tag="U2K71_002940"
     CDS             114..548
                     /locus_tag="U2K71_002940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316931.1"
                     /translation="MKNKIIKIITCLFLFGMIVGASTPLNLFPFSARGIKVEAASSRY
                     NHNHRGFTCYKMNYYVTNKMCRDLKKNYNKLKTPAQIASFIPIGGVGTWVITNAFSSA
                     VDAMNVFVRAANQGKGVQLTYNAHFSNTTSYQYNDYARYVIK"
BASE COUNT          316 a          119 c          144 g          311 t
ORIGIN      
        1 aaaataacac gttttataaa ataaaccctc ttgacaatat ttaattttct gattattcta
       61 aatatataca gaaagcgctt tcctaggatt ttatatctaa ggaggaagta acaatgaaaa
      121 ataaaattat taaaattatt acttgtctat ttttgttcgg tatgattgtt ggagcaagta
      181 ctccgttaaa tctgtttcct tttagtgcta gaggtataaa agtggaagct gcaagttcac
      241 gatataatca taatcatcgt ggatttactt gctataaaat gaactattat gttactaata
      301 aaatgtgtag agatttgaaa aaaaattata ataagctaaa gactcctgct caaattgcat
      361 cttttattcc tattggtgga gttggaacat gggttattac aaatgctttt agtagtgctg
      421 ttgatgcaat gaatgtcttt gtaagagctg caaatcaagg aaaaggtgta caattaacat
      481 ataatgctca ctttagcaat acaacatcat atcaatacaa tgattacgct cgttatgtaa
      541 taaaataatc agaaagtgaa actatctcat gaataaaaag attttagctg taattatttc
      601 aataatagtt ttaatcacta tgttcttcat atttaggcta attttcaata taagcttaca
      661 aaaaagtatt ctttatctaa ttcctatagc atttatctta gcaattttta gaagtattta
      721 cggaaataaa aactctaaat agctaaaaat aaatcatact aaattttaca aaaagtggct
      781 agcattttct agccactttt tgtattgtgg agcgtgggtt ttgaggggga ttcaaggggg
      841 agcttgcgcc cctttgacaa gcggttattt gcaccaaata cggaggtatt
//
LOCUS       DASHHB010000184          858 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.197, whole genome shotgun sequence.
ACCESSION   DASHHB010000184 DASHHB010000000
VERSION     DASHHB010000184.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 858)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 858)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..858
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.197"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..434)
                     /locus_tag="U2K71_002941"
     CDS             complement(<1..434)
                     /locus_tag="U2K71_002941"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7316932.1"
                     /translation="MEQLKAYKFRIYPTEEQEIFFAKSFGCVRKVYNLMLDDRKKAYE
                     EVKNDSSKKMTFPTPAKYKKEFPFLKEIDSLALANAQLNLDKAYKNFFRDKSVGFPRF
                     KSKKNPVQSYTTNNQNGTVALIDSKFIKVPKLKSLVRIKLHR"
     gene            complement(451..855)
                     /gene="tnpA"
                     /locus_tag="U2K71_002942"
     CDS             complement(451..855)
                     /gene="tnpA"
                     /locus_tag="U2K71_002942"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS200/IS605-like element ISEfa4 family
                     transposase"
                     /protein_id="HEM7316933.1"
                     /translation="MELDTNNHSVFLLYYHLVLVTKYRRQVIDDEISDYAKTTFERIS
                     ESYHITLVEWNHDKDHVHIMFKAQPKTELTKFINAYKSASSRLIKRDFPRVKQFLWKE
                     MFWSKSFCLLTTGGAPIDVIKKYIQNQGNNHK"
BASE COUNT          258 a          139 c          136 g          325 t
ORIGIN      
        1 tgtctatgaa gcttaattct tactaatgac ttcaatttag gaactttgat gaatttacta
       61 tcaatcaaag caactgtacc attttgatta ttcgttgtat aactctgaac aggatttttc
      121 ttacttttga aacgtggaaa tccaacggat ttatcccgaa aaaaattctt gtatgcttta
      181 tctaaattaa gttgggcatt ggctaaagca aggctatcaa tttctttcaa aaacggaaat
      241 tctttcttgt atttagctgg tgtcggaaaa gtcatttttt tagaagaatc atttttaact
      301 tcttcatacg ccttttttcg gtcatcaagc attagattgt agaccttacg gacacaacca
      361 aaagatttgg caaagaagat ttcttgttcc tctgttggat agattctgaa tttgtatgct
      421 tttagttgtt ccataaagtt cacctacttt ctatttatga ttgtttcctt gattttggat
      481 atacttctta atcacatcaa ttggtgcgcc accagttgtc agaagacaaa aacttttaga
      541 ccagaacatt tctttccaaa gaaattgttt gactcttgga aagtcacgtt ttatcaatct
      601 tgagctggca cttttatagg cattgatgaa ttttgtcaat tctgttttag gttgagcttt
      661 gaacataata tgaacgtggt ctttatcatg attccactcc actaaagtaa tgtgatacga
      721 ttctgaaatt ctttcaaaag tagttttggc ataatcagat atttcatcat caatcacttg
      781 tctacgatat ttcgttacta aaacaaggtg gtaataaaga agaaatactg aatgattatt
      841 tgtatctaat tccatgtt
//
LOCUS       DASHHB010000185          829 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.198, whole genome shotgun sequence.
ACCESSION   DASHHB010000185 DASHHB010000000
VERSION     DASHHB010000185.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 829)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 829)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..829
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.198"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..720
                     /locus_tag="U2K71_002943"
     CDS             <1..720
                     /locus_tag="U2K71_002943"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HEM7316934.1"
                     /translation="GKTELEETRLYNSMVLGMQNYYQIATNVNLDCMKLNRAVMAIFT
                     NRLGTNKRGRLRKTGRQLTTKEATRYGKSKMLRYVAGEGEPIFPVGYIQHRKPMAKVY
                     LANCYTLEGRKFIHTNLSVDKLLMYQLMKLSLENRTIEYADNRISLFSAQHGKCAITL
                     EEFQQANEIHCHHIVPTGAGGNDDYKNLILVKEAVHRLIHAKTEETIQKYIALLKLNK
                     VQLIRLNKYRVLAGNQELNLI"
BASE COUNT          319 a          128 c          165 g          217 t
ORIGIN      
        1 gggaaaacgg aattagaaga aacaagactt tataattcaa tggtattagg tatgcaaaat
       61 tattatcaga ttgcaacgaa tgtaaatctg gattgtatga aactgaatag agctgtaatg
      121 gctatcttca ctaacagatt aggtacaaac aaaaggggca gattgagaaa aacaggcaga
      181 caacttacga caaaggaggc aacaagatat ggtaaatcta aaatgcttcg gtatgttgct
      241 ggcgagggcg aacctatttt ccctgttgga tatatccaac acagaaaacc aatggcaaag
      301 gtgtatttag cgaattgcta cactctagag ggaagaaaat ttatccacac caatctaagt
      361 gttgataaat tactgatgta ccagttaatg aaattatctt tggaaaatcg cacaatagaa
      421 tacgcagaca atcgaatttc gctattttct gctcaacatg gaaaatgtgc tataactctg
      481 gaagaatttc aacaagctaa tgaaatacat tgtcatcata ttgtaccaac tggagctggt
      541 ggaaatgacg attataaaaa tcttattttg gtaaaagagg cggtacaccg tctaatacac
      601 gctaaaacag aagaaacaat acaaaaatat atagctctat tgaagctaaa taaagtccag
      661 ctcataagac taaataaata tagagtatta gctggcaatc aagaattaaa cttgatatag
      721 gtctactaca taaatacttt tcaatataaa cacgatggaa cgcggagtgc ggtgaaagtc
      781 gcacgctccg tgtgaatggg gggaaaagtt tgagatattt ataatatca
//
LOCUS       DASHHB010000186          822 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.199, whole genome shotgun sequence.
ACCESSION   DASHHB010000186 DASHHB010000000
VERSION     DASHHB010000186.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 822)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..822
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.199"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            22..>822
                     /locus_tag="U2K71_002944"
     CDS             22..>822
                     /locus_tag="U2K71_002944"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002301706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HEM7316935.1"
                     /translation="MPEIADEISILNLTSKSENQGLLDPYVIMKKKKDSESLAIDILT
                     FLTGISSRDGEKFPVLRKAIRTVTQSDQRGLLQVINELRKDGSPIAENIADHIESFTD
                     YDFAHLLFSDGSITQSISLDKQLNIIQVADLVLPDAETTFEEYTTMELLSVSMLIVIS
                     TFALDFIHSNREIFKIVDLDEAWSFLQVAQGKTLSNKLVRAGRAMNAGVYFVTQNSDD
                     LLDERLKNNIGLKFAFRSTDIHEIKKTLEFFGLDQEDQSNQKRLRDLEN"
BASE COUNT          253 a          142 c          185 g          242 t
ORIGIN      
        1 cgaggagggt ggaaagaaac cttgccagaa attgcggatg aaatcagtat cctcaacttg
       61 acgagtaagt cagaaaatca aggtctgctt gatccttatg tgattatgaa gaaaaagaaa
      121 gactcggaaa gtttagcaat tgatattctg acttttttaa caggaatttc aagtcgagac
      181 ggggagaagt ttcctgtgtt acgtaaagcc attcgaacgg tgactcaaag tgatcaacgg
      241 gggttattac aagttattaa cgaattgcga aaagacgggt caccaatcgc ggaaaatatc
      301 gccgatcata tcgagtcctt taccgattat gattttgctc atttgctttt ctcagacggc
      361 tcgattaccc aatcgattag tttagataaa cagctaaata tcattcaagt ggcggatttg
      421 gttttaccgg atgcagaaac gacttttgaa gaatatacca ccatggaatt gcttagtgtt
      481 tcgatgctaa tcgtcatttc cacttttgcc ttggacttta tccatagtaa ccgagaaatc
      541 ttcaaaattg tcgatttgga tgaggcttgg agttttcttc aagtggctca aggaaaaacg
      601 ttatcgaata agcttgttcg tgctggtcgg gccatgaatg ctggggtcta tttcgtcact
      661 caaaatagcg acgatttatt agatgaacgt ttaaaaaata atatcgggtt gaagtttgct
      721 tttcgcagta ccgatattca tgaaatcaag aaaaccttgg aattctttgg tttggatcag
      781 gaggatcaaa gcaatcaaaa acgcctgcga gatttggaaa ac
//
LOCUS       DASHHB010000187          806 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.200, whole genome shotgun sequence.
ACCESSION   DASHHB010000187 DASHHB010000000
VERSION     DASHHB010000187.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 806)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 806)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..806
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.200"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            175..806
                     /locus_tag="U2K71_002945"
                     /pseudo
     CDS             175..806
                     /locus_tag="U2K71_002945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611665.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3 family transposase"
BASE COUNT          265 a          153 c          155 g          233 t
ORIGIN      
        1 atgtgcaaga gcgtttaggc cactcttcta ttcaaatcat tatggatcta tatattcata
       61 ttacagacaa aagaaaagaa gaaaccgtag cgcaattcgc aaaatatagg ctctataatt
      121 tatgggactg atgaatcaaa attgattctt cagtcccatt gttattttag gtatatgatt
      181 cgtacattga aagagttgaa aaactatctt ccgaagccgt caaaaaggac cctgccaaaa
      241 gtattgatgg tggatgaatt tcgttcgcac gcttcaattg tggataaaat gagctttatt
      301 tgtgcggatg gcgagactgg aaaactcatt gatattttgc ctacacgaaa attgtctcga
      361 ttaacacact attttagtcg ctgtgcaaat cctgaagcgg tggaatattt agtgacagat
      421 atgaacgccg cctacttcca actgacaaaa cgtgtcttac caaatgcgaa agtcgtcatt
      481 gattgttttc atatcatcaa acatttgaat caggcgttca atgattttcg tgtacaagaa
      541 atgaaaagat tacaccagtt agggcaacga cgggaagccg agaaagtgaa aaagaactgg
      601 cgctttcttc taaaaaaccg tcaaaaaatc aatcactatg attacaaaac atggcgcagt
      661 tttcgtgcac ctaaataccc gctactcact gaagcaatga tgattgatcg attgctggca
      721 ttttcaacgc ctttaaagga cgcctatact tttttccatg agctgaccga agcctttaga
      781 gacaaggaat cagacttatt ttttac
//
LOCUS       DASHHB010000188          797 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.201, whole genome shotgun sequence.
ACCESSION   DASHHB010000188 DASHHB010000000
VERSION     DASHHB010000188.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 797)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 797)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..797
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.201"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..217)
                     /locus_tag="U2K71_002946"
     CDS             complement(<1..217)
                     /locus_tag="U2K71_002946"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007209032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7316936.1"
                     /translation="MTYTHLTPTELVMIEAYFNQSHSVSKVAHLVQRSRQTIYNVYRF
                     LKSGGSAISYYEQYKKNKRNCGRRPIVL"
BASE COUNT          297 a          112 c          113 g          275 t
ORIGIN      
        1 gtaacacaat cggacgtctt ccgcagttac gtttgttttt cttatattgt tcatagtagc
       61 ttattgcgct tccaccagat ttaagaaaac gatagacatt ataaatggtt tgtcgagaac
      121 gttgtaccag atgggctact tttgaaacag agtgtgattg attgaaataa gcttctatca
      181 ttacgagttc agttggtgta agatgggtat aggtcatttg tactcactcc ttataattct
      241 ttcgacggaa ctattttgag tgtagcacaa atgacttttt tagttgtcta gcttaatttt
      301 acaatcggcg aaggtaaaaa aataaaaaaa tgtgacaagt gttttgaatc agctgtggta
      361 aagtttcctc aatttaaact aaatccagct gcctcgatac aatttcaagg acatgtattt
      421 gatgttttag ctaaagataa cataaaaaaa tacaaaaaag gtaatatatt ccatattgat
      481 atacaaatat cttctaattt agaagatata aaaaaagcct ttaaaattaa aacaattggc
      541 actgctccta attttaaata acaaaaaaat tactagataa tctaaaaaca ggatggcatt
      601 atgagaacaa taactaaact tcctatatca catattttgt aattagtgaa aagcaaattg
      661 actactatca taagttaatg actctcatct taatatttaa aatatattca tatttttcta
      721 ttttatctaa atgcgatttt catatctaat aatagaaaaa taaacaatta acaataaaaa
      781 agtcttcatt tattgtt
//
LOCUS       DASHHB010000189          795 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.202, whole genome shotgun sequence.
ACCESSION   DASHHB010000189 DASHHB010000000
VERSION     DASHHB010000189.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 795)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 795)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..795
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.202"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..489)
                     /locus_tag="U2K71_002947"
     CDS             complement(<1..489)
                     /locus_tag="U2K71_002947"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HEM7316937.1"
                     /translation="MKDFQLGAATYLKSVAIRVKDRDKMINFYKNVVGFDLKREENEL
                     AILGTEEAGSEMLLLEESPRANDHMGVIKKLNRFSLIIPSTEEMADILCRIRKMNYPI
                     KEALEDHGRMGILLVDPEENELEIYHEENKNDQREIPETMDQEALLRKSEEKFSKLST
                     GSY"
BASE COUNT          219 a          157 c          124 g          295 t
ORIGIN      
        1 ataagaacct gttgatagtt tactgaattt ttcttcagat tttcttaata atgcttcttg
       61 atccattgtt tctggaattt ctctttggtc gttcttattc tcttcatggt aaatttccaa
      121 ttcattctct tctgggtcta cgagcaaaat ccccatacgt ccatgatctt ctaaagcttc
      181 tttgattgga taattcattt tacggatacg acaaaggata tctgccattt cttctgtgct
      241 agggataatc aatgaaaaac ggttcagctt tttgatcacc cccatgtgat cattcgctct
      301 aggactttct tccagcagca acatttcact tccagcttct tctgttccta aaatggctaa
      361 ttcgttttct tctcttttca aatcaaagcc aacaacgttc ttgtaaaaat taatcatttt
      421 atctcggtct ttcacccgaa tggctacact ttttaaatag gtggcagccc ccaattgaaa
      481 atctttcata tttcttgctc ccctctacgt caagctttat tttaacgatg gattcttctt
      541 ctttcaagca ataactttga ttttgttaaa aaaaatgtga gaaaattcgc attttgcttg
      601 ttttttgtaa attaatgagt aaaaactctt ataaaaagag gttgtgaaaa agctgttctg
      661 catcaagtaa taagaacagc caaacaaaaa tagctcctcc tatttttcgt ttggttgggc
      721 ttattaccgc agatgcaagc ttttgaacac cgtttaagga agaggttgtg aaaaagtcgc
      781 ttagtttcga gtagt
//
LOCUS       DASHHB010000190          779 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.203, whole genome shotgun sequence.
ACCESSION   DASHHB010000190 DASHHB010000000
VERSION     DASHHB010000190.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 779)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 779)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..779
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.203"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            233..>779
                     /locus_tag="U2K71_002948"
     CDS             233..>779
                     /locus_tag="U2K71_002948"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISL3-like element ISEfa5 family transposase"
                     /protein_id="HEM7316938.1"
                     /translation="MNDSIKKMLRLIEKDLMITEVSYETFQKKKTLIVDAVFSPAPHT
                     CRNCGSTVVDGNGKVIVVKNGKKETIVRFEQYNHMPLVMRLKKQRYTCKNCRTHWTAQ
                     SYFVQPRHSIANHVRYKIASLLTEKVSLSFIAKNCQVSLTTVIRTLKEFKSYLPKQSK
                     RILPRVLMVDEFRSHASIEDKM"
BASE COUNT          259 a          138 c          145 g          237 t
ORIGIN      
        1 atttcaaatt gcgcatcatg attcgtcatg gcaacgcatt gattttcaac taaaaaagat
       61 caagaggaac tgatccactt gatctccaat tattctggct ctataattta tgggactgat
      121 gaatcagaat tgattcttca gccccatttt tattttaggt attaaaaaac atatcgttct
      181 ctagtatgat taaatcacga caaaataaac aactgagagg acgatatgct atatgaatga
      241 ttctatcaaa aaaatgctgc gattaataga gaaagatttg atgattacag aggtctctta
      301 cgagaccttt cagaagaaaa agacgttgat cgtcgatgct gttttctcac ctgctcccca
      361 tacctgtaga aattgtggtt ctactgtggt agatggaaac gggaaagtaa ttgtagtgaa
      421 aaatgggaag aaggaaacga ttgtccgctt tgaacaatac aatcacatgc ctttggttat
      481 gcgcttaaaa aagcagcgct atacctgtaa aaactgtcga acccattgga cggctcaaag
      541 ttattttgtt caacccagac attcaatcgc gaatcatgtc agatataaaa ttgcttcttt
      601 actgactgaa aaagtatctt tatcttttat tgcgaaaaat tgtcaggtat ctttgaccac
      661 cgttattcgt acgttgaaag agtttaaaag ctacttacca aagcaatcta agaggattct
      721 ccccagagta ttgatggttg atgaatttcg ttcgcacgct tccatagaag ataaaatga
//
LOCUS       DASHHB010000191          771 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.204, whole genome shotgun sequence.
ACCESSION   DASHHB010000191 DASHHB010000000
VERSION     DASHHB010000191.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.204"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..660
                     /locus_tag="U2K71_002949"
     CDS             <1..660
                     /locus_tag="U2K71_002949"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM7316939.1"
                     /translation="YISLLCKEEIIELPKTNSTIGIDLGIMDFAILSDGQKIDNNKFT
                     SKMEKKLKREQRKLSRRALLAKNKGTNLFEAKNYQKQKRKVARLHEKVMNQRTDFLNK
                     LSTEMIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSKFVTKLQYKADWYGRKIIKV
                     DKWFPSSQICSECGHKDGKKSLEIREWTCPVCHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          285 a          118 c          143 g          225 t
ORIGIN      
        1 tatatttctt tgttgtgtaa agaagaaatt atcgaattac ctaaaactaa ttccacaatt
       61 ggtattgact taggtattat ggactttgcg attctttctg atggacaaaa aattgataat
      121 aataaattca cgtccaaaat ggaaaagaaa ctaaagcgtg aacaacgcaa attgtctagg
      181 cgtgctttat tggctaaaaa taaaggtacc aatctatttg aagctaaaaa ttatcaaaaa
      241 caaaaacgca aagtagctag attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat gatcaaaaat cacgatatta tctgtattga agacttaaac
      361 acaaaaggta tgttgcgaaa tcataaatta gctaaaagta tttctgatgt ttcttggtca
      421 aagtttgtga ctaaattaca atacaaggct gactggtatg gacgaaaaat catcaaagta
      481 gataaatggt ttccgtctag tcagatttgt tcagagtgcg gacataaaga tggcaagaaa
      541 tctcttgaaa ttcgagaatg gacttgtcct gtttgtcata ctcatcatga tcgtgatatc
      601 aatgctagta tcaatattct gaccgaaggt ctaagactac attctatggg attggcttag
      661 atccaatcat caagaaccgt aggaactacg gggatagctt ggtaaataag agacaccgct
      721 gttagtaaag aaatacgcta tcaagtatgc tctattccca agaagctccc a
//
LOCUS       DASHHB010000192          767 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.205, whole genome shotgun sequence.
ACCESSION   DASHHB010000192 DASHHB010000000
VERSION     DASHHB010000192.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 767)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 767)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..767
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.205"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            33..>767
                     /locus_tag="U2K71_002950"
     CDS             33..>767
                     /locus_tag="U2K71_002950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000997695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS256-like element ISEf1 family transposase"
                     /protein_id="HEM7316940.1"
                     /translation="MNDFTTEIVQTLVTKGDLNELFRSHLEKAINTLLRTELTAFLDY
                     EKYDRTGFNSGNSRNGSYFRSIKTEYGELTLEIPRDRNGEFKQQTLPAYKRTNDTLET
                     TIIHLFEKGVTMSEIADLIEKMYGHHYTPQTMSNMTKVLTEEVNAFKSRALNDKYVAI
                     FMDATYIPLKRQTVSKEAIYIAIGIREDGTKEVLSYAIAPTESTYVWNELLQDINSRG
                     VQEVLLFITDGLKGMKDTIHQIYPKAK"
BASE COUNT          279 a          144 c          132 g          212 t
ORIGIN      
        1 gacaaccaat tcacagaaaa gaggacttcc ctatgaatga ttttactaca gaaattgtgc
       61 aaactctagt cactaaaggc gatttaaatg aattattccg ttcgcactta gaaaaagcga
      121 taaacacact cctacggact gaattaacgg cttttttaga ttacgaaaaa tatgatcgca
      181 ctggttttaa ttcaggtaat tcgagaaacg gttcttactt tcgatcaatc aaaaccgaat
      241 atggtgaatt aacattggaa atacctagag atcgtaatgg tgagtttaaa caacaaactt
      301 taccagccta caaaagaaca aacgatacat tggaaaccac tattatccat ttattcgaaa
      361 aaggtgttac gatgtctgaa attgctgatt tgatcgaaaa aatgtacggt catcactata
      421 ctccacaaac catgtccaac atgactaaag ttctgactga agaagtaaat gcctttaaat
      481 ccagagcctt aaatgataag tatgtcgcta tttttatgga cgctacttac attccactaa
      541 aacgtcaaac cgtatccaaa gaagcgattt atattgccat tggtatacga gaagacggca
      601 ctaaagaagt actgagttat gcgattgctc caactgaatc aacatacgtt tggaatgagc
      661 tgctacagga tattaactcc agaggagttc aagaagtctt gctttttatt acggacggct
      721 taaaaggcat gaaagatact atccatcaaa tttatcctaa agcaaaa
//
LOCUS       DASHHB010000193          757 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.206, whole genome shotgun sequence.
ACCESSION   DASHHB010000193 DASHHB010000000
VERSION     DASHHB010000193.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 757)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..757
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.206"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(318..>757)
                     /locus_tag="U2K71_002951"
     CDS             complement(318..>757)
                     /locus_tag="U2K71_002951"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HEM7316941.1"
                     /translation="KKAEGSPCFLLYQTFPGIGALTAALLLGELGDITRFKTHKQLNA
                     FVGIDIRRYHSGKYTGQDRINKRGNPKARKIIFFTIRNMIRQQRAAPNHIVDYYYKLK
                     KQPIPKKDKVATVACMNKLLKCMHAMVRAHTEYDYAYAVSVDH"
BASE COUNT          233 a          147 c          161 g          216 t
ORIGIN      
        1 aaaaagagcg tgggacaaaa gtaaagaaca cttttgtctc acgctctaaa cacgtataaa
       61 cggcggaagc agaagcaact ccttcggaaa taagctgaaa ttcacaaaaa tttaaaaagc
      121 aattttcttg agtttcttct tatttcttgg agttaaacgc ttctgtccca gcctcttttc
      181 ctacgattag tcaagtaaaa ggtgcaagaa aaatagctcg taaacgaaag cgacaaaatc
      241 aaagcacacc aaataaagcc ccgttcgtaa aaaattttcg atctgggcaa agaagtggta
      301 tactgtaagt ggtttagtta atggtccact gagaccgcgt acgcatagtc gtactctgta
      361 tgtgccctaa ccatagcgtg catacatttc agaagcttgt tcatacaagc aactgtggca
      421 accttatctt tcttagggat aggttgcttt tttaatttgt agtaataatc gacaatatga
      481 ttaggagctg cgcgttgctg gcgaatcata tttcgaatgg tgaagaaaat aattttccgt
      541 gctttgggat taccacgctt attgatgcgg tcttggccag tatacttacc tgaatgatac
      601 cgtcggatgt cgattccgac aaaggcattc aattgtttat gggttttaaa tcgtgtgata
      661 tctcctaatt caccgagcaa taaagcggct gtgagtgcgc caatcccagg gaacgtttgg
      721 tacaacaaaa agcaaggaga gccttcggct tttttga
//
LOCUS       DASHHB010000194          756 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.207, whole genome shotgun sequence.
ACCESSION   DASHHB010000194 DASHHB010000000
VERSION     DASHHB010000194.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 756)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..756
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.207"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U2K71_002952"
     CDS             complement(138..491)
                     /gene="tnpB"
                     /locus_tag="U2K71_002952"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001846105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS66 family insertion sequence element accessory
                     protein TnpB"
                     /protein_id="HEM7316942.1"
                     /translation="MLYDLLQTKHIYIVCGKTDLRKGIDGLASLIQQEYQLELYEDAV
                     FLFCGNRQDRFKLLYWDGDGFLLCYKRIENGKLKWPRTKDEVRTLTNQQVRWLLEGLS
                     IDQPRAILPGKKGVF"
     gene            complement(481..675)
                     /locus_tag="U2K71_002953"
     CDS             complement(481..675)
                     /locus_tag="U2K71_002953"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002285758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316943.1"
                     /translation="MYKLNEIVPVQLGNEKSAPEKGKPAAAKLAMRIKRENGSEVLIY
                     NGINNYILQAILKELLSNAL"
BASE COUNT          225 a          160 c          130 g          241 t
ORIGIN      
        1 ttgaagcaac tgaagcaacg tatctgtagt ttccaaaatt tttcaccacc ttgaatcgaa
       61 ctgattttgt tacagaatac cacaagaaac gcacaagaaa taggggttca acacattttt
      121 tgtgttaacc ccccagctta aaaaactcct ttttttcctg gcaaaatagc tcgaggttga
      181 tcgatactta atccttctaa caaccatcga acctgttggt tcgttaaggt tcgaacttcg
      241 tctttcgtcc gaggccattt taacttgccg ttttctatcc gcttataaca gagaagaaaa
      301 ccatcaccgt cccaatagag aagtttgaaa cggtcttgac ggttcccgca aaataaaaaa
      361 acggcatctt cgtaaagctc gagttgatac tcttgttgaa taagactggc aagaccatca
      421 attccttttc gcaaatctgt ttttccgcac acaatataaa tatgtttggt ttgaagtaaa
      481 tcataaagca ttggacaaca gttctttcaa aatagcttga agaatataat tgttaatacc
      541 gttatagatc aaaacttcag aaccattctc tcttttgatg cgcatcgcta acttggcagc
      601 cgcgggttta cctttttctg gtgcactctt ttcgtttcct aattggacag gaacaatctc
      661 atttaattta tacatagaaa acctccagac ttcttgatag ctcaagtata tcttgaggtg
      721 catttgttag taaggtactg ttctatgggg cgctta
//
LOCUS       DASHHB010000195          742 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.208, whole genome shotgun sequence.
ACCESSION   DASHHB010000195 DASHHB010000000
VERSION     DASHHB010000195.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 742)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 742)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..742
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.208"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            178..381
                     /locus_tag="U2K71_002954"
     CDS             178..381
                     /locus_tag="U2K71_002954"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucocin A/sakacin P family class II
                     bacteriocin"
                     /protein_id="HEM7316944.1"
                     /translation="MKHCVILGILGTCLAGIGTGIDVDAATYYGNGLYCNKEKCWVNW
                     GQSWSEGLKRWGDNLFGSFSGGR"
     gene            381..731
                     /locus_tag="U2K71_002955"
     CDS             381..731
                     /locus_tag="U2K71_002955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002338811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bacteriocin immunity protein"
                     /protein_id="HEM7316945.1"
                     /translation="MTSINIDHTKKVIHELYNSLMTRPNQTKALLDITDVLFQVYKKI
                     DTAKYPEILVNKLVNYIYIVGFDNKIRFIDNDEKLLIELGDISKKAGINSKYKANFTD
                     KSQFYSYSETMPQR"
BASE COUNT          300 a           79 c          120 g          243 t
ORIGIN      
        1 gacttccttt tttgtatgga ttttttcatt tacacaaaat attttacact ctcgtcaatc
       61 attgttatat aataaaatgt tatataataa aattactaat gatatttaat attacaagca
      121 atcttaagaa attcgaaaag gaaaaaatga ttatgaaaaa gaaagaaaaa gaaagtattg
      181 aaacattgtg ttattctagg aatattagga acttgtctag ctggcattgg tacaggaata
      241 gacgttgacg cagctactta ctatggaaat ggtctttatt gtaacaaaga aaaatgttgg
      301 gtaaattggg gacaatcatg gtcagaaggg ttaaaacgtt ggggagataa tctttttggt
      361 agttttagtg gaggacgata atgacttcaa taaacattga tcacacaaaa aaagtcatac
      421 atgaattata caatagttta atgacacgac ctaatcagac gaaagcatta ttagatatta
      481 ctgatgtttt atttcaggta tataaaaaaa ttgatacagc aaaatatcct gaaattcttg
      541 taaataaatt agttaattat atttacatcg tgggttttga taataaaata cgctttattg
      601 ataatgatga aaaattatta atagaattag gggatatttc taaaaaagct ggaattaata
      661 gtaaatacaa agcaaacttt acagataaat cgcaatttta tagttattca gaaacaatgc
      721 ctcaacgata aaatatagta ta
//
LOCUS       DASHHB010000196          716 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.209, whole genome shotgun sequence.
ACCESSION   DASHHB010000196 DASHHB010000000
VERSION     DASHHB010000196.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 716)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 716)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..716
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.209"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            141..398
                     /locus_tag="U2K71_002956"
     CDS             141..398
                     /locus_tag="U2K71_002956"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316946.1"
                     /translation="MSNLENKEEKVVNKIVSVVNKLDKELDELDTLSENPEKKHNLKK
                     WLVERKAIHEIKKVLHEADKYEKYDEKELDKEFKEINDLLL"
     gene            complement(630..>716)
                     /locus_tag="U2K71_002957"
     CDS             complement(630..>716)
                     /locus_tag="U2K71_002957"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002363335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4231 domain-containing protein"
                     /protein_id="HEM7316947.1"
                     /translation="DEENFHLIVTTLQTELNDIISDFNQVNN"
BASE COUNT          288 a           86 c           97 g          245 t
ORIGIN      
        1 ttctgtccac gttcatttta ctccaaagtt ttcctcattt caaaagaaaa tcacagaata
       61 cacgaagaag cgtttctatt tgtataaact atatcaaagt tctatactta aattatcaaa
      121 taaaaaggac gtgaagataa atgtcaaatt tagaaaacaa agaagaaaaa gttgtaaaca
      181 aaattgtttc tgttgtcaat aaactggata aagagttaga cgaacttgat acgttatctg
      241 aaaatccaga aaaaaaacat aacctaaaaa aatggttagt tgaacgaaag gctattcatg
      301 aaattaaaaa agttcttcat gaagctgata aatatgaaaa atatgatgaa aaagaacttg
      361 ataaagaatt taaagaaatc aatgacttat tactataatt gatttatacg actcattttt
      421 gagtcgtcag actgcaaaca acactctaaa tttaggagtt tgtctacagt ctgaatagat
      481 tttaaattta ttgtccataa tgttattatg aatttatagc attaagaaaa gagataagac
      541 tttacatttt tttaggaatg taaagtctta tctcttttct taattgtaca attagtatta
      601 tctgcactac tttctaatta aataaatttt tagttattaa cttgattaaa atcagagata
      661 atatcattta attcagtttg aagggttgta acgattaaat ggaaattttc ttcatc
//
LOCUS       DASHHB010000197          702 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.210, whole genome shotgun sequence.
ACCESSION   DASHHB010000197 DASHHB010000000
VERSION     DASHHB010000197.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 702)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..702
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.210"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>702
                     /locus_tag="U2K71_002958"
     CDS             <1..>702
                     /locus_tag="U2K71_002958"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS30 family transposase"
                     /protein_id="HEM7316948.1"
                     /translation="DGQQEYIQKKVAQGWTPDVLIGRAEFPIACSVRTLYRMFKDKTF
                     DPHDLPMKGKRKPNGHKEKRGKQAFRRSIQDRTTDYIQFNHEFGHLEGDTIVGGKHKS
                     AVITLVERLSKVIITLKPAGRQAIDIENRLNQWFQSVPKHLFKSITFDCGKEFSNWKK
                     ISNTNDISIYFADPGTPSQRGLNEHSNGLLRKDGLYKAMDFNKVSETFIQSVASKRNH
                     IPRKSLHYRTPLEVF"
BASE COUNT          246 a          124 c          139 g          193 t
ORIGIN      
        1 agatgggcaa caagaataca ttcaaaaaaa ggtggctcaa gggtggactc cagatgtcct
       61 tattggtcga gcagagtttc ctattgcttg ttcggtacgt acactatatc ggatgtttaa
      121 agataaaacc tttgaccctc acgatttacc aatgaaaggg aaaagaaaac caaacggaca
      181 taaagaaaaa agaggaaaac aagcttttcg ccgttcgatt caggatagaa caactgatta
      241 tatacagttc aatcatgagt ttggtcacct agaaggtgac accattgtcg gtggaaaaca
      301 taaaagtgcg gtgatcacgt tagttgagcg attatctaaa gtgatcatca ccttgaaacc
      361 cgctggtagg caagctatag atattgagaa tagactgaat caatggtttc aatctgtacc
      421 gaaacacttg tttaaatcca tcacatttga ttgtggaaaa gagttctcga attggaaaaa
      481 gattagtaat accaatgata tttcgattta tttcgctgat ccaggaacac cttctcaacg
      541 aggactaaat gaacattcga atggcttatt acgtaaagat ggcttatata aagctatgga
      601 ttttaataag gtatccgaaa cctttatcca atcggtggca tcaaaacgaa atcatattcc
      661 tagaaaatca ttacactatc gcacaccatt ggaagtattt tt
//
LOCUS       DASHHB010000198          659 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.211, whole genome shotgun sequence.
ACCESSION   DASHHB010000198 DASHHB010000000
VERSION     DASHHB010000198.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 659)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 659)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..659
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.211"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>659
                     /locus_tag="U2K71_002959"
     CDS             <1..>659
                     /locus_tag="U2K71_002959"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002349331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HEM7316949.1"
                     /translation="YISLLCKEEVRELPKSNSAVGIDLGIIDFAILSTGQKFDNNKFT
                     SKMEKKLKREQRKLSRRALLAKQKGINLFEARNYQTQKRKVARLHEKVMNQRTDFLNK
                     LSTEIIKNHDIICIEDLNTKGMLRNHKLAKSISDVSWSSFVSKLQYKADWYGRKIIKV
                     DKWFPSSQICSNCGHKDGKKSLEIREWTCPICHTHHDRDINASINILTEGLRLHSMGL
                     A"
BASE COUNT          236 a          111 c          119 g          193 t
ORIGIN      
        1 tatatttctt tgttatgcaa agaagaagtt cgcgaactac ctaaatctaa ttctgctgtt
       61 ggtattgacc taggtattat agactttgca attctttcta ccggtcaaaa atttgataat
      121 aataaattta cgtccaaaat ggaaaagaaa ctcaaacgtg aacaacgtaa gttgtcaaga
      181 cgtgccttat tagctaaaca aaaaggtatc aatctgtttg aagctagaaa ttatcaaaca
      241 caaaaacgta aagtagcacg attgcatgaa aaagtaatga atcaacgtac tgattttctg
      301 aataagttga gtacagaaat tatcaaaaat cacgatatca tctgtattga agacttaaac
      361 acaaaaggca tgttgcgtaa tcataaacta gcaaaaagta tttcggatgt ttcttggtct
      421 agttttgtgt ctaaattaca atacaaggct gactggtacg gtcggaaaat catcaaagta
      481 gataagtggt ttccatctag ccaaatctgt tcgaattgtg gacataaaga cggcaagaaa
      541 tctctcgaaa ttcgagaatg gacttgccct atttgtcata ctcatcatga ccgagatatc
      601 aacgctagta tcaatatcct gaccgaaggt ctaaggctac actctatggg attggctta
//
LOCUS       DASHHB010000199          651 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.212, whole genome shotgun sequence.
ACCESSION   DASHHB010000199 DASHHB010000000
VERSION     DASHHB010000199.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 651)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 651)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..651
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.212"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(<1..357)
                     /locus_tag="U2K71_002960"
     CDS             complement(<1..357)
                     /locus_tag="U2K71_002960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate transporter"
                     /protein_id="HEM7316950.1"
                     /translation="MATVWMGLALVGNTFSPNSAMEGQGVIDVLTHIYTTGPAEYAKS
                     ILVNVFFGAFFGRVLVDSGIAATLIRKVVELGGDKPRITMSLLCIVTAVIFMSMTGIG
                     PVISIAVIVLPILMSLG"
BASE COUNT          210 a          148 c          104 g          189 t
ORIGIN      
        1 acctaatgac atcaagatcg gcaacacaat aactgcaata gaaatcactg gtccgattcc
       61 tgtcatcgac atgaatataa cagcggttac aatacatagt aagctcatcg tgattcttgg
      121 cttgtcccct ccaagttcaa ctacctttcg aatcagggta gctgcgattc cgctatctac
      181 tagaacacgc ccgaaaaagg caccaaagaa gacatttacc aagatcgatt tggcgtactc
      241 agctggcccc gtcgtataga tatgcgtcaa cacatcaatt actccttgcc cttccatcgc
      301 cgagtttggt gaaaacgtgt ttccgactag cgctaatccc atccatacag tagccatgat
      361 gacaaaagcc gaccatcaag ttgtagcctt ttacacagta ccagaccatg aaaaagaatg
      421 agataactaa caaaatacca ataataatat cactcatgtt attcctccgt ccattttgtt
      481 tttctgataa gcaaactaat atatcagtca aactcattct aacttatgta gttagcgctt
      541 tcattaaagg atgaccataa atgttcagtt ttcaattaaa aaaccttaat tatgttattt
      601 tagacttata cgttattgtc ctgtttcatt tgatggagga ttgtaaataa a
//
LOCUS       DASHHB010000200          621 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.213, whole genome shotgun sequence.
ACCESSION   DASHHB010000200 DASHHB010000000
VERSION     DASHHB010000200.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 621)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 621)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..621
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.213"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            92..310
                     /locus_tag="U2K71_002961"
     CDS             92..310
                     /locus_tag="U2K71_002961"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002322165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316951.1"
                     /translation="MDLYDIESGATLSVESDHLIINFGGKRQIILWVVDDVLFPEIVH
                     DFEESKAVEFEIVKKVMELIEKYEEDSE"
     gene            331..420
                     /locus_tag="U2K71_002962"
                     /pseudo
     CDS             331..420
                     /locus_tag="U2K71_002962"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002293000.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator"
     gene            495..>621
                     /locus_tag="U2K71_002963"
     CDS             495..>621
                     /locus_tag="U2K71_002963"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ArpU family transcriptional regulator"
                     /protein_id="HEM7316952.1"
                     /translation="MVLFDVKKYETPSAKDVDMERTKHNVAVFLSAYLSARCRVGQ"
BASE COUNT          227 a           79 c          146 g          169 t
ORIGIN      
        1 gatcgtttca aatttgtaga gaagtataaa gaaatattag aagagtattt gaggaggaca
       61 gcaaatgata aaaaaactcg ttcaattcag catggattta tatgatatcg aatcaggagc
      121 aacactatct gtggaatcgg accatctaat cataaatttt ggtggaaagc gccagattat
      181 tttgtgggta gttgatgatg tactgtttcc agaaattgtt catgatttcg aagaatcaaa
      241 agcggttgag tttgaaatag tgaaaaaagt aatggaattg attgaaaaat acgaggagga
      301 cagcgaatga taccgaagtt tagagcgtgg gatagagaaa aagacagaat gaaggcagta
      361 gctttggttg aatttgctga ggcgtataaa tcaggagaac tcatttcacg tacaaaataa
      421 aaaaagtcgg attcctccga ctatgaataa tatttccgac ataagtatta tatcataatt
      481 gggggaatca gaggatggta cttttcgatg taaagaaata tgagacacca agcgcgaaag
      541 atgttgatat ggaacgcaca aaacataatg tcgctgtgtt tctttcagca tatctatcag
      601 ctagatgtag agtagggcaa c
//
LOCUS       DASHHB010000201          609 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.214, whole genome shotgun sequence.
ACCESSION   DASHHB010000201 DASHHB010000000
VERSION     DASHHB010000201.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 609)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 609)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..609
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.214"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>609
                     /locus_tag="U2K71_002964"
     CDS             <1..>609
                     /locus_tag="U2K71_002964"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002312603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182-like element ISEfa7 family transposase"
                     /protein_id="HEM7316953.1"
                     /translation="KIPHFSTFGKNYVRRFRETTVFEDIFAYILEQAVKAGFVTEDNL
                     YLDSTHIKANANKHKFTKEMTHDEAKAYQDELEDEINRQRIEAGKRPFTLDLEKEVKL
                     KERKISKADPESGYYVKGEREKQFAYSVHTSCDDNGFILSTIITPRNIHDSQVAFQLV
                     KQSKRLFPEINCVVADAGYKTPKFVHFLTHLNLRPCLPYSRPK"
BASE COUNT          205 a          116 c          118 g          170 t
ORIGIN      
        1 aaaatccccc atttctcaac ttttggaaag aattatgttc gccgatttag agaaacgacg
       61 gtgtttgagg atatttttgc ctatatttta gaacaagcag ttaaagcagg ttttgtaaca
      121 gaggataatt tgtatctgga ttctacccac atcaaagcta acgccaataa acataagttt
      181 acgaaagaaa tgacccatga tgaagcgaaa gcctatcagg atgaattaga agatgagatc
      241 aatcgtcaac gaatagaggc tggaaaacgc ccttttactt tggacttaga aaaggaagtg
      301 aagctgaaag aaagaaaaat aagtaaagct gatccggaaa gcggctacta cgtcaaaggc
      361 gaacgagaaa aacaatttgc ttattctgtc catacttcat gcgacgacaa tggatttatt
      421 cttagcacaa tcatcactcc tagaaatatt catgacagtc aagtcgcctt tcagctcgtc
      481 aaacaatcga agagactttt tcctgaaatc aattgtgttg tcgctgatgc tgggtataag
      541 acacctaagt ttgttcactt tttgactcat ttgaaccttc gaccttgttt gccttattca
      601 aggcccaaa
//
LOCUS       DASHHB010000202          573 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.215, whole genome shotgun sequence.
ACCESSION   DASHHB010000202 DASHHB010000000
VERSION     DASHHB010000202.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 573)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 573)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..573
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.215"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
BASE COUNT          185 a           81 c           81 g          226 t
ORIGIN      
        1 tttttcttga tgtgatatcc ttgtcttttt tgtaatgttt ttaacttatt gcttttgcaa
       61 aatttattta cctgttttct ggatcaacga tagtgtacat tatgctatcc ttgtttggat
      121 tttaggacgt ttaaagaggc acttttttac ataacttaat ccatagacct tttaagcatg
      181 ctgtgagata ataataaaga agagtttaaa gcgcacccca aaccacttcc ccataagtgt
      241 attacgcttt aaactctttt atatttgaat tcactaagaa gcataccata tttttgaaaa
      301 aaagtgagaa aaaaggctta taattaggat ttagagtaat taattagatg tttattttat
      361 aggactgata ctatagatta caaatagtat cgtatttcgc aatcttaaaa ttatcttctt
      421 actgtgaata agataataaa atttgtattt gtccttttta ggtaacttat tttaatctat
      481 atacctttct ggatcaacga aagtatactt tatatagtca taacgccgat ggtaactatg
      541 cactgtaacg ataatgctgc taaaatatct ttt
//
LOCUS       DASHHB010000203          555 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.216, whole genome shotgun sequence.
ACCESSION   DASHHB010000203 DASHHB010000000
VERSION     DASHHB010000203.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 555)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 555)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..555
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.216"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..>555
                     /locus_tag="U2K71_002965"
     CDS             <1..>555
                     /locus_tag="U2K71_002965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IS3-like element ISEnfa3 family transposase"
                     /protein_id="HEM7316954.1"
                     /translation="LMSQMGLTCQIRIKRYKSYKGTVGKIAKNVLKRNFSVDTPNKKW
                     VTDVTEFKIKGRKIYLSPILDLFNGEIISYSISTSPTYKLIEEMLQQAIKKKGTEGSL
                     ILHSDQGWQYQMPQYQKKLKENNIIQSMSRKGNCLDNSVIENFFGVLKSEFFYREKFR
                     SIEIFQSKLNEYIRWYNNKRIKLK"
BASE COUNT          237 a           70 c           91 g          157 t
ORIGIN      
        1 attaatgtcc caaatgggac ttacctgtca aatccgaata aagcgatata aatcttataa
       61 aggaacagta ggaaaaattg ccaagaatgt gttaaaacga aatttttcag tagatacacc
      121 caataaaaaa tgggtgacag acgtcactga attcaagata aaaggaagaa aaatctatct
      181 atctcctata cttgatttat ttaacggaga aataattagt tatagtatat caactagtcc
      241 aacatataaa ctgattgaag aaatgcttca acaagcaatt aaaaaaaagg gaactgaggg
      301 ttcactgatt ctacattcag atcaaggatg gcagtatcag atgccacaat atcaaaaaaa
      361 attaaaagag aataacatta ttcaaagcat gtctagaaaa ggaaattgct tagataattc
      421 tgtaatagaa aatttttttg gtgtgcttaa atcagagttt ttttatcgag aaaaattccg
      481 atcaattgag atatttcaaa gtaaattaaa tgagtatatt aggtggtata ataacaaaag
      541 gataaaacta aagtt
//
LOCUS       DASHHB010000204          532 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.217, whole genome shotgun sequence.
ACCESSION   DASHHB010000204 DASHHB010000000
VERSION     DASHHB010000204.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 532)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 532)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..532
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.217"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            313..429
                     /locus_tag="U2K71_002966"
     CDS             313..429
                     /locus_tag="U2K71_002966"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010768364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1972 domain-containing protein"
                     /protein_id="HEM7316955.1"
                     /translation="MIGLNAISAKYCKFKAFVEKFTERQTDKIIQYHITYTD"
BASE COUNT          225 a           50 c           70 g          187 t
ORIGIN      
        1 ataataaaga attgaacttc tgagaaaaca aagaatcttt tttctttttt tgaattgact
       61 catggattgg cgttaaaatt ttattttgaa aaagttattt ctggtttaac tcaaaaaaag
      121 agatatgaat ctaatagtga tagaaaggat gttatttttc atgaaatggt tgttttagat
      181 ttaatacata taactaataa atgctattga atgtatttaa actaattaac aaaagaattt
      241 gaattgttaa taaaatccaa taaaatatat taataaagta gatagaaata aaaaaacaaa
      301 gactaaacat atataattgg attaaacgca atatctgcaa aatattgtaa atttaaagcc
      361 tttgtagaaa aatttactga aagacaaact gataagatta ttcagtatca tattacctat
      421 actgattaaa attttttgaa acgggatact aaataaaaaa tttatttata acagaactaa
      481 ttatttaatg cggatgcgcc aatacttggt cctgcaagag ctatttattt tg
//
LOCUS       DASHHB010000205          515 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.denovo.218, whole genome shotgun sequence.
ACCESSION   DASHHB010000205 DASHHB010000000
VERSION     DASHHB010000205.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 515)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 515)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..515
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.denovo.218"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(107..>515)
                     /locus_tag="U2K71_002967"
     CDS             complement(107..>515)
                     /locus_tag="U2K71_002967"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002342103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="linear amide C-N hydrolase"
                     /protein_id="HEM7316956.1"
                     /translation="GTPENHFSNQISLNVYSRGMGGLGLPGDLSSVSRFVKATFTKMN
                     AASGDSESESISQFFHILGSVEQQKGVCDTGEGKYEYTIYSSCCNVDKGIYYYRTYED
                     SQITAVNMNKEDLDRHELVSYPVIKEQQINYVN"
BASE COUNT          163 a          103 c           78 g          171 t
ORIGIN      
        1 atcatccata aaatggactt tgtctacagt ctgagacacg acctaaagat cgtgtctaat
       61 tatgactgta tcagaaaata tgataccaaa cggatctatt cattaattaa ttgacatagt
      121 ttatctgttg ctcttttatg actggataac tgaccaattc atgacgatcc aagtcttctt
      181 tattcatatt tacagcagta atttgactgt cttcatacgt acgataataa taaatccctt
      241 tatcaacgtt acagcaagaa gaataaatcg tatattcgta ttttccttct ccagtatcgc
      301 atactccttt ttgttgctcg actgagccta gtatatgaaa aaattgactg atactttcag
      361 attctgaatc acctgatgca gcattcattt tagtaaatgt ggcttttaca aatcgagaaa
      421 cagaagagag atctccaggt aagcccaatc ctcccatccc acgactataa acattcaagc
      481 tgatctggtt tgaaaaatga ttttctggtg ttcca
//
LOCUS       DASHHB010000206        28608 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.032, whole genome shotgun sequence.
ACCESSION   DASHHB010000206 DASHHB010000000
VERSION     DASHHB010000206.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 28608)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 28608)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..28608
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.032"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(172..2310)
                     /locus_tag="U2K71_002968"
     CDS             complement(172..2310)
                     /locus_tag="U2K71_002968"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM7316957.1"
                     /translation="MQKKEIAVFIDQITRERATGDLLIVGWAIDEVTKEIPTIKVEKE
                     NVIAEATHVVRLDINHLYNLDVKTQSGFKVRLSGKMRGKAILDFQTARHQNGIAVKLN
                     GKYPYDDGIESSWERKKRLLKKGINYARTHGVKKAIRRVKLELKPGSIDYAEWISRHE
                     KPDLKEQRKEAQGFAYRPLISILMPVYNVEIKWLEKCIDSVLDQTYDHWELCISDDAS
                     TDPAIRKCLESYQAKDDRIKVVFRKENGHISLATNSALEIAEGEFIALLDNDDELPPF
                     ALYEVAKVLNVHPELDVIYSDEDKIDADGNRFDPHFKADWSPDTLMGNNYISHLGVYR
                     TSIVKELGGFRKGYEGSQDYDLVLRVTEQIPADHIYHIDRVLYHWRTIPGSTASNGEA
                     KSYIYDSGVKALTDALSRRNIKGSVRPGRISGFYEIAYDVLQEDLVSVIIPTKNGYED
                     LKTCVDSIIEKTSYSNYEIIIADNGSTDPKMQELFAEYKHQLKDRFIVELIDIPFNYS
                     RINNLAAEKANGKYFLFLNNDTEVIEPDWMTVMVSYAQFDRIGCVGAKLFYPDDTTQH
                     AGVLLGIGGVAGHALNNYDRTHCGYFGRLVIDVNYLAVTAACMMVKAADFNAVNGFDE
                     TLEVAFNDVDLCLKVYELGRYNVYAHQAELYHFESKSRGYEDTPEKQRRFAGEIKKMQ
                     DKWPAYIAHDPFYNDNLTKEGIGDFSLRPD"
     gene            complement(2349..3935)
                     /locus_tag="U2K71_002969"
     CDS             complement(2349..3935)
                     /locus_tag="U2K71_002969"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HEM7316958.1"
                     /translation="MKYDFEMDLDEQSSVGKIAAQIKPGSKVLEFGPGNGRLTKHLIG
                     AKQCEVSIVELDKELFDFVSEFAQDGFYGDIESFEWANYYAGQTFDYVLFADVLEHLV
                     DPGKTLKKVREFLNEEGEILITFPNLAHNSVMIDLFNNQLPWASYGLLDETHNSFYTH
                     DGFQKVFEKAGLFINIEDYLYLAVGDTELKSTYEELPEAVRYDFKMRPFGEVYQYFFS
                     LMKHPVAQSSIAEPQNSNYVKVLEVTQQTKQDETIQQIPFNNFTGENETLSFPVSETT
                     ERMVFRFAQQPSFIEFSAEAAGEKLTFIDSNAVVKTVNDCYLFDGKELPEFVLTDISG
                     KEVTIHCHYRFIGELTPTMKELLETIRPMAEVTKQLSEKNDELERENHHLLEENTRLD
                     HTLKTTTNRYCTLLESDEWTIKHRLGRRKKETSKKIQEKELSICIDEKIWDAETKILK
                     IIGWGIANSDRQPLSYKLSADQSPFFEAIPVSREEVNQAEQLAKGTEAGFELRILCEQ
                     ERSFLVEAVAQNGQSWIIEM"
     gene            complement(3925..5145)
                     /locus_tag="U2K71_002970"
     CDS             complement(3925..5145)
                     /locus_tag="U2K71_002970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HEM7316959.1"
                     /translation="MTEYAIRLKDITKSYNMYAKPSDRFREALNPFKKSYHDVFYALK
                     NVNVEIKKGEMIGFVGENGSGKSTMLKIITGVLTPTSGTMEIEGKISALLELGSGFNP
                     EYSGYENIYLNGMVLGFSREEVDEMVDDIIEFADIGDHIYQPVKTYSSGMFVRLAFAV
                     AINVNPDILIVDEALAVGDLEFQLKCMEKFTEIKNSGKTILFVSHDISSIRRFCDRSY
                     WLKNGEVVEYGDTMEVTTNYENFLKKKSVKTVDRNKNQVERFAAPDIVDVTKAELLDK
                     DKNPVDMLEQGEKLYVKVTYEVKDDTIKNPVLGIALRTVDNFYVCGLNTLLDGIKIPW
                     KKGENIFYLEYEKLGLLAGEYYFDVAVFEENATVPLVYKTKYMNLFVSGSYIGEGIVV
                     LDHKWEEGTHSNEI"
     gene            complement(5157..5963)
                     /locus_tag="U2K71_002971"
     CDS             complement(5157..5963)
                     /locus_tag="U2K71_002971"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HEM7316960.1"
                     /translation="MFKDLYLFMKDIYQNRKLLLQFSINDFKSRYAGSFLGILWAFIN
                     PLFTVLIYWLVFGVGLKAQMIDAKYPFIVYLVTGMIPWFFFSDAFASTTVVFREYTYL
                     VKKVVFNIRILPTTKILSNLYTHLFFILIGIAVALINGIYPSAMSLQLIYYLFCMCAF
                     LTGLTWLTASIQPFLPDIMQLITIVLQLIMWTLPILWSPNQFDSGLVSILKLNPLYYV
                     VQGYRESFLSEAWFWEHGTYTLYFWVFTLVMFVIGSAVFRRLKPHFSDVL"
     gene            complement(6173..7453)
                     /locus_tag="U2K71_002972"
     CDS             complement(6173..7453)
                     /locus_tag="U2K71_002972"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EpaQ family protein"
                     /protein_id="HEM7316961.1"
                     /translation="MEIIQAIVQKFVNKMSVGFLLLLLTLVFFVWTAGLGFPATLFIY
                     NNSSLLIAFSVAILLILNVYKAKGVDFLYLGVALALFIFFSYFRELRNESLFGDLLFP
                     LIILFCLVIKWIEWTKWDRGIYLIVFSVFLGITLYRVFTEIQVPEGQSIWAPGNSLSD
                     IWINTNIIGSSLMTLALLISGFASSFERWYVRIIGAVAVVAAFLGIWVCQSRGALVAL
                     IVFAILDLLPKSLMRVIRAPFIAYTVTILLALPISYLAAVSEKVNLFTGREDIWHKFY
                     QTLGEKSEQILLGMKTFIFQRGNQFLGNHNSYNSILNIYGLIGFGIAALLLILFIGRL
                     TLKADLSNGQMTFIWAFFAVMMQSFMEDTLTSYPWVPIIYILLAMAVHRYDEPEVAEE
                     FYVMEEAYDDMTYDDTSYEEQPDSAHSSRIQRYH"
     gene            complement(7497..9530)
                     /locus_tag="U2K71_002973"
     CDS             complement(7497..9530)
                     /locus_tag="U2K71_002973"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7316962.1"
                     /translation="MEKQTRSARRKEKPKANQADSNLVLDLFLKLRYLIGVLVIVVIV
                     TFNLNGSSLGVWDKYVSQRDDGKKTDVIFGQNREVRSDEWLVQTPFYLSQAEEDFPLV
                     NEDYSLNGQNMIIAYNSPVKDITVIGKPFNWGFFFLGRDRGLSFYWAMKLVVMVLLGF
                     EVLMILTKRKKALSLIGAFALTFSPAVQWWFMQHVGDLIFFTLGLMTAFYHYFYNHDK
                     KWLRTLMMLLVVIFGIGFILVIYPAHQVMLAYLLIFYFVGLLIYYSKKVSWDLLDVVL
                     IGGAVLFIGGVMVHFWLTSKDALLASLNTLYPGKRVSTGGKWTIGEFFSFLTNWKIPF
                     EDINYSNNSEVALFYHFFPTVFLASPFVLFGKKDSEQKLLGRILMIFCLFSIFWITVG
                     LPKWLAEITLLSYVPPLRAILTFSFAACLLSIWFIAYIWREDVIPIWYKFILLAVNAW
                     SYFYAITHSKMDNYFSDFETIAVAVLGTVILAFALFGWRRLFYLAFAALVIVSGFFVN
                     PVVEGTGAIFEKTLAKEIQKIDQQDPGKVWLSEDELYNFTPTLGVKSFNTVRFYPDIA
                     AWKKIDPKEEYEKYYNRYAHTRAFIAKEDTKFILDRPDMFSVTLNFEDAEKLGIDYVI
                     SKRPLTDYNQLYEAQFTQLYGPDKDGYMIFKVEYPDYPDVPQTQTQENQLVVQ"
     gene            complement(9564..10415)
                     /gene="rfbD"
                     /locus_tag="U2K71_002974"
     CDS             complement(9564..10415)
                     /gene="rfbD"
                     /locus_tag="U2K71_002974"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002286428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HEM7316963.1"
                     /translation="MFLLTGGNGQLGTELRHLLDEKNLQYVSTDAKDLDITDAEKTMA
                     YITELKPDVIFHCAAYTAVDKAEEEAKELDEKINVDGTRNVALAAKAVNAQFVYISTD
                     YVFDGKNKNVEYEVDDQTNPLNEYGRTKLLGEKAVEEILEDYYIIRTSWVFGIYGHNF
                     VFTMQRLAETRDKLTVVNDQFGRPTWTRTLAEFMVYVIEEKAPFGIYHLSNENSCSWY
                     EFAKEILKDTDVEVAPVTSEEFPQKATRPQYSVMSLKKTEELGFVIPTWQEALAQMLE
                     AAKKMNK"
     gene            complement(10513..11541)
                     /gene="rfbB"
                     /locus_tag="U2K71_002975"
     CDS             complement(10513..11541)
                     /gene="rfbB"
                     /locus_tag="U2K71_002975"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HEM7316964.1"
                     /translation="MKNIIVTGGAGFIGSNFVHYVVNNHPEVHVTVLDKLTYAGNKEN
                     LAGLPSDRVELVVGDVADAELVDRLVKEADAVVHYAAESHNDNSLKDPFPFVQTNIIG
                     TYTLLEACRKYDVRYHHVSTDEVYGDLPLREDLPGHGEGEGEKFTAETPYNPSSPYSS
                     TKAGSDLLVKAWVRSFGLRATISNCSNNYGPYQHIEKFIPRQVTNILSGIRPKLYGEG
                     KNVRDWIHTNDHSSAVWLILTKGRIGETYLIGADGEEDNKTVMELILEMMGQPKDAYD
                     HVNDRAGHDLRYAIDSTKLREELGWKPEFTNFRDGLADTIKWYEEHEDWWKKEKEAVE
                     AAYAKNGQ"
     gene            complement(11563..12135)
                     /gene="rfbC"
                     /locus_tag="U2K71_002976"
     CDS             complement(11563..12135)
                     /gene="rfbC"
                     /locus_tag="U2K71_002976"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010771301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HEM7316965.1"
                     /translation="MKVTDTKLTDVKIVEMDVFGDHRGFFTESYSKAKFAEHGLKYDF
                     VQDNHSLSAEAGVLRGLHFQKGEAAQTKLIRVVTGAVLDVVVDIRKGSPTYGQWDGFI
                     LSEHNHRQLLVPKGYAHGFVTLTPNVNFLYKCDNYYNAEADGGIAFDDPDLAIDWPID
                     PSRAIMSEKDHRHPTLKEFEAENPFVYGEI"
     gene            complement(12149..13015)
                     /gene="rfbA"
                     /locus_tag="U2K71_002977"
     CDS             complement(12149..13015)
                     /gene="rfbA"
                     /locus_tag="U2K71_002977"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010735945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HEM7316966.1"
                     /translation="MKGIILAGGSGTRLYPLTKATSKQLMPIYDKPMIYYPMSTLMLA
                     GIKEILIISTPQDTPRFKELFGNGNDLGLQIEYAVQESPDGLAQAFIIGEEFIGDDSV
                     CLVLGDNIYYGGGLSKMLQRAASKDTGATVFGYHVNDPERFGVVEFDENMQALSIEEK
                     PAQPKSNYAVTGLYFYDNEVISIAKGIKPSERGELEITDVNKAYLDKGKLSVELMGRG
                     FAWLDTGTHESLLEASTFIETIEKRQNLKVACLEEIAYRMGYIDKEQLLALAQPLKKN
                     QYGQYLLNLAAE"
     gene            complement(13115..13849)
                     /locus_tag="U2K71_002978"
     CDS             complement(13115..13849)
                     /locus_tag="U2K71_002978"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HEM7316967.1"
                     /translation="MLRIGRCEFVRSVCIATFNGGRYLREQLDSILSQLSAEDEVVIS
                     DDGSVDDTWDILTEYAEKDKRIQLFSGPRQGLIANFGYAIQQTKGDLIFLADQDDVWL
                     ETKVKKITDYFEQNPDQLVVVSDLIIVDSELQEIHDSYFSFRNSKEGWLNNVIRSHYI
                     GAGMAFRSSLKPDILPIPSEVPMHDMWIGLLGGKKTGFLREPLTLYRRHDLNASEIET
                     HSKLRQKISWRVHLITALIKRKWFKR"
     gene            complement(13827..14654)
                     /locus_tag="U2K71_002979"
     CDS             complement(13827..14654)
                     /locus_tag="U2K71_002979"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002377110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM7316968.1"
                     /translation="MKPEILVVVVLYQQLFSQSPAYGPLSKALSEKQIQLIIYDNSPQ
                     RQQNELFEKENIVYHHDPQNPGLAVAYNFALSQADEGTRCLVTLDQDTRLVDDYFELL
                     RKVTFTDECVAAVPMIFSGSRQVSPLYSDRYINRTAQAVEVEGPTAERIMAINSGVAW
                     SVKFLKKIGGFNPAFSLDFLDHWLFWKVDQLEKTVEILPVRMEHDLSVLDYEKVSSNR
                     YRSILQAENRFYHEYDQRHLSSHRRQLLLRTAKQFLTVKNRHIWRMTLRSYVKNWKV"
     gene            complement(14654..15454)
                     /locus_tag="U2K71_002980"
     CDS             complement(14654..15454)
                     /locus_tag="U2K71_002980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002346778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HEM7316969.1"
                     /translation="MNNKLNQPILISIVTANSDKIFRTLDNFISTVKDETAVKIRIFD
                     NNSLPEYKKRLTEYRKYPYIDIYFHDENSGFGYGHNHNLIASDSRYGIICNPDILVDE
                     ETVTALVLLLKEHPECAMLAPKILNEDGTTQHLIREQLAVFDYVLRFVPFKWVKKKFD
                     KRLAVFECRGLPTDANSYVRMISGSFMVADIDKFKEIKGFDERFFMYFEDNDLCLSFE
                     KAGYKLLYTPFYAVIHFYGKGAHRNAKLFRVFLTSMMKFFNKWGWRFF"
     gene            complement(15447..16586)
                     /locus_tag="U2K71_002981"
     CDS             complement(15447..16586)
                     /locus_tag="U2K71_002981"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002329034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl/decaprenyl-phosphate
                     alpha-N-acetylglucosaminyl 1-phosphate transferase"
                     /protein_id="HEM7316970.1"
                     /translation="MLVSFSMVIRFFLTILLSLILTPVFKIISVQTGMVDKPNERRIN
                     KVPMPSAGGLPIFISFVISTLFLFRKIIPQSYIVPILIASLVIIVTGVLDDKYELTPK
                     QKSIGILIASLIIYFVADIRIDSFTLPFIGYIQLGWLSFPVTIFWIFGITNAVNLIDG
                     LDGLAAGISLIGLITIGIIGYFFLHASTVYIPVVIFCLVASIIGFFPYNFYPAKIYLG
                     DTGALFLGFMMAVLSLQGLKNVTFVSSISLLIVMGVPVTDTFFAIIRRKANRVSFSTA
                     DKKHLHHRLISLGFTHKGAVLTIYAMALMFSFTAMVMNYTGRIGTIILIIAMLFAAIL
                     LFELIGLINEKHQFILKTLRFLGNKEYRTQVKQKYLKKWNRHIDE"
     gene            complement(16791..17984)
                     /locus_tag="U2K71_002982"
     CDS             complement(16791..17984)
                     /locus_tag="U2K71_002982"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HEM7316971.1"
                     /translation="MKEKRRDNKGRILHTGESQRTDGKYLYKYVDALGNTKYVYAWRL
                     TPTDPTPKGKREKPSLRELEQQIRRDIEDGIDSTGKKMTLCQLYAKQNAQRTNVKKST
                     QKQREQLMRLLKEDKLGARSIDTIKPSDAKEWALRMKDKGFSYNTINNHKRSLKASFY
                     IAIQDDYVRKNPFDFKLSEVIENDTKEKVALTEEQEQALLSFIKTDNVYHKHYDDVLI
                     LLKTGLRISELCGLTVADIDFKNEVVIIDHQLLKSKEQGYYIETPKTKSGIRQVPLSK
                     ETIQAFQRVINSRPKIKSIEVDGHSGFVFINPKGNPKVAIDYNALFIRMVKKYNKHHK
                     DNPLPHITPHTLRHTFCTRLASKNMNPKDLQYIMGHSNISITMNWYAHASIDTAKSEV
                     QRLIA"
     gene            complement(18063..18269)
                     /locus_tag="U2K71_002983"
     CDS             complement(18063..18269)
                     /locus_tag="U2K71_002983"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002347229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="excisionase"
                     /protein_id="HEM7316972.1"
                     /translation="MSNTQDIPVWEKYTLTIEEASKYFRIGENKLRRLAEENKDAGWL
                     IMNGNRIQIKRRQFEKVIDKLDAI"
     gene            complement(18262..19404)
                     /locus_tag="U2K71_002984"
     CDS             complement(18262..19404)
                     /locus_tag="U2K71_002984"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005336080.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HEM7316973.1"
                     /translation="MNNLEFALEMKNKRLASGLSQGELASLTHVSRYSINRFENGKAN
                     ASKETQNIILRCLNYYICDKPFYLLVDYLSVRFPTTDALEVIRKVLGMKADYFIHYDY
                     GYYGYKEHYAYGEIKVMASDDEHMGVFLELKGAGSRNMEYVLQAQNRDWYSFLNRCLD
                     CGGVIRRFDLAINDMCGLLDIPVLSEKYRNGGAECRCKNYENVQGGKLSGKNRNLAST
                     LYIGSKASTKYFCLYEKQKEQATKKKHTDIINRFEIRLRDKKAVQAVEELLLTYNPHG
                     LVFYLITDFVEFPDYPLWEIFISHDSLPFEMNPVPVNMERTLQWLERQVMPSIVMIEE
                     IDRLTGSNYMKMIDECTHLSEKQEMLVEQMCTDISDVIESEGVFYE"
     gene            19916..20551
                     /locus_tag="U2K71_002985"
     CDS             19916..20551
                     /locus_tag="U2K71_002985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003127055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7316974.1"
                     /translation="MEGYAMKISHLGNNIQTIRKFRGMKQQELADKIGINMQSLSKIE
                     RGLNYPAYETLEKIMEVLDVTPNELLSGEWKYVNQSEKEVCQFLRAEERLNAELKHGH
                     YDNFFDSEEEWLEYELEKLREYITDYINGKSIEASDLYPIKEFIQHLKFQKLLDRYDD
                     LYSMDMFGESIEGHKYKTPYQVVKMINPNSKEDMELLREVLRNNNFDDEDE"
     gene            20893..21528
                     /locus_tag="U2K71_002986"
     CDS             20893..21528
                     /locus_tag="U2K71_002986"
                     /inference="COORDINATES: protein motif:HMM:NF012655.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7316975.1"
                     /translation="MARNKYPEETVKLILDVSTKMFCEKGYDNTSLQDIINETKLSKG
                     AIYHHFDSKEDILKAIFQRIGNENAVIFAKIRDNDSLNGLEKLREIFKTAVFNSNQSV
                     LLTVSPQLLNNPRFLAMQIEQMYEIVVPEFVEPVLEQGIKDKSLEIENSHEIAEVIMV
                     LSNVWLNPLVEMTDKEGMEKRCNTFNALLNGIGIDTLLDEETISAFIQFCK"
     gene            21635..22873
                     /locus_tag="U2K71_002987"
     CDS             21635..22873
                     /locus_tag="U2K71_002987"
                     /inference="COORDINATES: protein motif:HMM:NF017760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7316976.1"
                     /translation="MQAEKKLFNRDFILVVIGQIISLFGNAILRFALPYYLLKETGSS
                     ALFGIATACSFLPMVILSLVGGIIADRTNKRNIMVILDFVTAIIILVFYLSLGKIPII
                     PLFIVALMLLYGISGAYQPAVQASIPLLVSSDKLTVGNAIINQVNTLANFIGPAISGV
                     VFQFYGINPILIVSIICFTFSAIMEIFIHIPHQKRPREVGVIAVAKNDLKDSYTFLKK
                     EKPIFISVIFLVCIFNLVLSAVLIVGLPILISEVLKMPDTLYGFSQSALSLGGLCGGI
                     LTALVASKLKLKNLYTLLLICSVSVLLMGISLFLELPAIVSYWVVTVMCFVAMGVSTM
                     FVVQIYTLVQTQTPPQLVGKIMATLVAVAMCGQPIGQMVYGILFDVFIQETWVVMFVA
                     SIASIMISIYSKRIFQKLEI"
     gene            23100..23519
                     /locus_tag="U2K71_002988"
     CDS             23100..23519
                     /locus_tag="U2K71_002988"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009588541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HEM7316977.1"
                     /translation="MRTSSFEHIVRLQFNSLMLIVIKNKVKSRNRQLARHSKREVLFC
                     EIAETKQVERGTIDTCFYDCVSFKVLHFTIYVSDETLGTVLHRLSEKQRSAILLRYFQ
                     GMNDREISELYHVSRSAICSRRTRGLRKLKMLLSERK"
     gene            23524..23763
                     /locus_tag="U2K71_002989"
     CDS             23524..23763
                     /locus_tag="U2K71_002989"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004609551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HEM7316978.1"
                     /translation="MSKDYGYPSFELICKASSGDEMAVKEILKFYDGYISKLCLRPFY
                     HSESGKIIMQVDEELKGEIHTDMMKAILKFEIGVK"
     gene            complement(23988..24911)
                     /locus_tag="U2K71_002990"
     CDS             complement(23988..24911)
                     /locus_tag="U2K71_002990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002330638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HEM7316979.1"
                     /translation="MKVWNKIKQNENLMRFIETTQKRITDSEMSTTSVVVAYYLLLSL
                     FPLIIAFGNILPFLHIDQETVLTYLREVIPETVYQFIGPAIKDLLTQSSGGLLSVSAL
                     AALWSASQSINALQIAMNKAYGVENRKNFLIVRFFSLIVIVLFMIAISGVTIVLGLGQ
                     LILDAIQPIVQIPVNFIDQFQTLKWPITLVALFVIMFLIYLIVPNAQLKLKTIIPGTI
                     FATVGWMLLSQVFSIYARYFATRISGYQIIGSFIVLMLWLNFAATIIILGGIINAVVQ
                     EYVTGHQIKKRRDPSRKWFNKLKTKFSHKKK"
     gene            complement(24943..25707)
                     /gene="map"
                     /locus_tag="U2K71_002991"
     CDS             complement(24943..25707)
                     /gene="map"
                     /locus_tag="U2K71_002991"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008377375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HEM7316980.1"
                     /translation="MITLKSAREIEMMAESGALLADVHKNLRDFIKPGITSWDIEVFV
                     RNYIESHGGIAAQIGFEGYEYATCISINDEICHGFPRKKPLKNGDLVKVDMCIDLKGG
                     ISDSCWAYVVGESTPEIDRLMEVTKKALYIGIEQAQVGNRIGDIGHAIQTYVEGENLA
                     IVRDFIGHGVGPTIHEEPAVPHYGEAGKGLRLKEGMVITIEPMVNTGTWKMKMDPNGW
                     TAYTRDGGLSCQYEHTLAITKDGPRILTSQGEEGTY"
     gene            25865..26305
                     /locus_tag="U2K71_002992"
     CDS             25865..26305
                     /locus_tag="U2K71_002992"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002297963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin"
                     /protein_id="HEM7316981.1"
                     /translation="MALAKIVYASMTGNTEEIADIVAEAFENLDIEVEINECTQVDAD
                     EFEEADICVVATYTYDDGDLPDEIVDFYEDLQELDLLGKIYGVCGSGDTFYDEFCKSV
                     DDFDAAFAKTGASKGAENVKVDLNAEEEDIENLEAFAKELVAKI"
     gene            complement(26483..27052)
                     /locus_tag="U2K71_002993"
     CDS             complement(26483..27052)
                     /locus_tag="U2K71_002993"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002339630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HEM7316982.1"
                     /translation="MARKKTITREQILAAAYEVAATDGFTRFTARNIANKMNCSTQPI
                     YLEFKNMDDLRQALFEQIYDHLQYEVFTVEHTGNTIIDLAFNYIHFARREKKLYRSLY
                     LEEYGGGKEMQNFSYTYFVNTVKRDPAYVNLSEEQIESLYNGTWIIATGIAALMSSSI
                     IHPTDEQIEKMIQDSIDAILKFKEPIEID"
     gene            27307..28539
                     /locus_tag="U2K71_002994"
     CDS             27307..28539
                     /locus_tag="U2K71_002994"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HEM7316983.1"
                     /translation="MLSDFNELLKKYARLIAETGVATEKGHTVVLQISVEQAPLARLI
                     TQEAYKLGAAEVIVQWTDDQIQREFLLHAATDRIENVPQSKIDQADEWLEKGASRISV
                     VSADPDAFAGVDSHRVASYQSANGKALMNLRKATQANKVSWTVVAAAGKQWAAKVFPE
                     LPEEKQVEALWDQIFKTTRVYEEDPVLAWKKHDEKLAKKAEELNREQFSALHYTAPGT
                     DIIIGLPKNHLWEGAGSYNARGEKFMANMPTEEVFTAPDSHRVDGYISSTKPLSYAGT
                     IISGMKFTFKDGKVVDFSAEQGEDVLAKLLDTDEGARRLGEVALVPDPSPISQSGIIF
                     FNTLFDENASNHLALGSAYAFSVKGGTEMSDEELAEAGLNRSQTHVDFMVGSDKMDID
                     GIREDGSTVPIFRNGDWA"
BASE COUNT         8688 a         5751 c         5008 g         9161 t
ORIGIN      
        1 aaatttgaaa agcaattttc gtgaatttct tcttatttct cggagttaaa cacttctgtc
       61 ccaacctcga cttatttttt tgagtcggca ccttctgcct ttttttgaca aaaaactata
      121 aaaagccgtt atgtgcgagc gaatcacaac ataacagctt tttattatag tttaatctgg
      181 tctcaacgaa aagtcgccga ttccttcttt tgttaaatta tcattgtaaa atggatcatg
      241 cgcaatatat gctggccatt tatcttgcat ttttttgatt tcaccagcaa aacgtctttg
      301 tttttctgga gtatcttcat aacctcttga tttagattcg aaatggtaca attctgcttg
      361 atgtgcatag acattgtaac gacccagttc ataaactttc aagcacaagt caacatcgtt
      421 gaatgctact tctaatgttt catcaaaccc gttaacagca ttgaagtcag ctgccttgac
      481 catcatacaa gctgcagtga ctgctaagta attgacgtca atgaccaatc ggccgaaata
      541 accacaatgt gttcgatcat agttattcaa tgcatgccct gcaacaccac cgattcctaa
      601 taatactcct gcatgctgcg ttgtatcatc tggataaaac agttttgctc ctacacagcc
      661 gattcgatca aactgtgcat aagaaaccat caccgtcatc caatctggct caatcacttc
      721 tgtatcgtta ttcaagaata aaaagtattt gccgtttgct ttttctgctg ctaaattgtt
      781 aattcgagaa taattgaatg gaatgtcaat caattctacg ataaagcggt ctttcagctg
      841 atgcttgtat tctgcaaata attcttgcat ttttggatct gtacttccgt tatccgcaat
      901 aatgatttca taatttgaat aagacgtttt ttcgatgatc gaatccacac aagtttttag
      961 atcttcatag ccattcttcg ttggaatgat cacactgacc aaatcctctt gcaagacgtc
     1021 ataagcaatc tcataaaatc cagaaattct gccaggacgt acgcttcctt tgatgtttct
     1081 tctagacagg gcatctgtca atgctttcac tccggaatca tagatataac ttttggcttc
     1141 gccattactt gcagttgatc ctgggatcgt ccgccaatga tacaagactc ggtcaatgtg
     1201 gtaaatatga tctgcaggga tttgttctgt cacacgaagg accaaatcat aatcttgaga
     1261 accttcatag cctttacgga aaccgccaag ttctttaaca atgcttgttc gatatacgcc
     1321 taagtgagag atataattat ttcccatcaa tgtatcaggc gaccagtctg ctttgaaatg
     1381 cggatcaaag cgattgccat ctgcatcgat tttatcttcg tcactgtaga tcacatccag
     1441 ttctggatgt acgtttagca cttttgccac ttcatataat gcaaatggcg gtaattcatc
     1501 atcattatct aagagtgcta taaattctcc ttctgctatt tccaatgcag aattagttgc
     1561 cagactgatg tgtccatttt cttttcggaa aacgactttg attctatcat cttttgcttg
     1621 ataggattcc agacactttc ggatagctgg atcagtagaa gcatcatcac tgatacatag
     1681 ttcccaatga tcatacgttt gatccagtac cgaatcaatg catttttcga gccatttgat
     1741 ttcaacgtta tagacaggca taagtatgga aatcaatgga cggtacgcaa atccttgtgc
     1801 ttctttcctt tgctctttca aatctggttt ctcatgacga ctgatccact ctgcatagtc
     1861 gatcgatcct ggcttcaatt ccaatttgac ccgacggatc gcttttttta cgccatgagt
     1921 ccttgcataa tttatccctt ttttcagtaa tctttttttt ctttcccatg aagattcaat
     1981 gccgtcatca taagggtatt taccgtttaa tttcacagct atgccatttt gatgtctcgc
     2041 tgtttggaag tctaagatgg cttttcctct cattttccct gataaacgta ccttaaaacc
     2101 gctttgggtc ttaacatcaa gattatataa atgattgata tccaatctta cgacatgtgt
     2161 tgcttcagca atcacatttt ctttttctac ctttatcgta gggatttctt tggtcacttc
     2221 atcaattgcc cagcccacaa taagcaaatc gccagtggcc cgttcacgag tgatttgatc
     2281 gataaatacg gcaatttctt ttttttgcac gattcgttta ctccttatct ttataagtta
     2341 atcattattt acatttcaat gatccatgat tggccatttt gagctactgc ttccactaag
     2401 aatgaacgtt cttgctcgca aaggattcgt aactcaaatc ccgcttctgt tccttttgcg
     2461 agctgttctg cttgattgac ttcttctcgt gaaacaggaa ttgcttcgaa aaatggcgat
     2521 tggtcagctg ataacttata ggacaagggc tggcgatccg aattagcgat tccccatccg
     2581 atgattttta atatttttgt ttctgcatcc cagattttct cgtcaataca tatagaaagt
     2641 tctttttctt ggattttttt cgaagtttct ttcttacgtc gtcctagtct atgttttatt
     2701 gtccattcat cactttccaa caatgtgcaa tagcgatttg tggttgtttt taacgtatgg
     2761 tctagtcttg tattttcctc aagaagatga tgattttctc tttctaattc gtcatttttc
     2821 tcagaaagct gctttgtgac ttcagccatg ggacgaatcg tttccaacaa ttctttcatt
     2881 gttggtgtca attccccgat aaagcgatag tgacaatgga tagtcacttc tttcccagaa
     2941 atatccgtta gaacgaactc tggtaattct ttcccatcaa acaaataaca atcatttact
     3001 gttttgacta cagcattcga atcgataaat gttagttttt ctccggcagc ttctgcgctg
     3061 aattcgataa agctaggttg ttgcgcgaag cgaaacacca ttctttctgt tgtttctgat
     3121 acggggaaac ttagtgtctc attttcccct gtgaaattat tgaatggtat ttgttggatt
     3181 gtttcatctt gtttcgtttg ctgggttact tctaacactt ttacataatt agaattttgt
     3241 ggctctgcaa tggaactttg tgctactgga tgtttcatca atgagaaaaa gtattggtag
     3301 acttcaccaa atggtcgcat tttgaaatca tagcgtacag cttccggcaa ttcttcataa
     3361 gttgatttta attctgtatc acctacagct aagtacaaat aatcttcaat attaatgaat
     3421 aatcccgctt tttcaaaaac tttttggaag ccatcatgag tataaaaaga attatgggtt
     3481 tcatctagga gaccataaga agcccacggc aattggttat tgaacaaatc gatcatgaca
     3541 gaattatgcg ccaagttagg gaatgtaatc aggatctctc cttcttcatt caaaaattct
     3601 ctgactttct tcaatgtttt tccaggatct accaaatgtt ctaaaacatc cgcaaacaag
     3661 acgtaatcaa atgtctgtcc agcataatag tttgcccact caaaactttc gatatcacca
     3721 taaaatccat cttgagcaaa ttcgctgaca aaatcgaaca attccttatc taactcaaca
     3781 atgctcactt cacattgttt tgctccgatt aaatgtttcg ttaatcggcc gtttccggga
     3841 ccaaactcta aaactttact tcctggtttg atctgtgcgg caatttttcc cacactgctt
     3901 tgttcatcta ggtccatttc aaaatcatat ttcattgcta tgagtccctt cttcccattt
     3961 atggtctaag accacgatgc cttctccgat ataagaaccg ctgacaaaca agttcatgta
     4021 ctttgtctta tataccaaag gaaccgttgc attttcttca aagaccgcaa cgtcaaaata
     4081 atattcacca gctaacaaac ctaacttttc atactccaga taaaagatat tctctccttt
     4141 tttccaagga atcttgatac catctagtag agtattaagt ccgcatacat aaaaattgtc
     4201 gactgttcgt agcgcaattc ctaaaaccgg atttttgatt gtatcatctt ttacttcata
     4261 agttacttta acgtatagtt tttctccttg ttccagcata tccaccggat ttttgtcttt
     4321 atccaacaat tccgctttcg tcacgtcgac gatatctgga gcggcgaacc gttctacttg
     4381 gtttttattc cgatccactg ttttaactga ttttttcttt aaaaagtttt cataattcgt
     4441 cgttacttcc atcgtatcgc catattcgac gacttctccg tttttcaacc aataggaacg
     4501 atcacaaaaa cgacgaatcg aactgatatc atgagaaacg aataaaatcg tctttcccga
     4561 gttcttgatt tctgtaaatt tttccataca ttttagttga aattcaagat cgcctactgc
     4621 caatgcttca tccacaatca aaatatcggg attcacattg attgctacag caaaagctag
     4681 acgaacgaac atcccgcttg aataagtttt tacaggttga taaatatggt ctccgatgtc
     4741 agcaaattca atgatatcat cgaccatttc atcgacttct tctcttgaaa atcctagaac
     4801 cattccattt aaatagatat tttcatagcc agaatactca ggattgaatc ctgatcctag
     4861 ttctagcaaa gctgagattt ttccttcgat ttccatcgtg ccggatgttg gtgtcagtac
     4921 tcccgtaatg attttcaaca ttgtagattt tcccgatccg ttttctccca cgaaaccgat
     4981 catctcacct ttttttattt ctacattcac gtttttcaac gcataaaata catcatgata
     5041 ggatttctta aaaggattga gggcttcacg aaatcgatcc gaaggttttg cgtacatatt
     5101 ataagatttt gtgatatctt ttagtctaat tgcatattct gtcatagcta ttctccttac
     5161 agcacatctg aaaaatgtgg tttcaatcta cggaacactg cagagccgat tacgaacatt
     5221 actaacgtga aaacccagaa atacaaagta tatgttccat gctcccagaa ccaagcttca
     5281 cttaaaaatg actcacgata accttgaaca acatagtaca gcggatttaa tttcaatatg
     5341 cttacaagac ctgaatcaaa ctgattaggg ctccataata tcggtaatgt ccacatgatc
     5401 aactgcagca cgatcgtaat taattgcatg atatcaggta aaaatggttg gatcgatgcc
     5461 gtcagccaag tcaatcctgt caagaaagca cacatgcaaa ataaataata aatcagctgt
     5521 aaagacattg ctgaaggata tataccattt ataagcgcga cagctatgcc aatcagaatg
     5581 aagaataagt gggtgtacag attggatagg attttcgtcg tgggcaagat acgaatattg
     5641 aatacgactt tctttactaa ataagtatat tcccgaaaaa caaccgtagt tgatgcaaat
     5701 gcatctgaga agaagaacca gggaatcatc ccggtaacta aatatacgat aaatgggtat
     5761 tttgcatcga tcatttgtgc tttcagtcca acaccaaata ccagccaata aatcaataca
     5821 gtaaatagcg gattgataaa cgcccataag attcctaaaa acgatcctgc atatcttgat
     5881 ttgaagtcat tgatcgaaaa ctgtagcaat aattttctgt tctgataaat gtccttcata
     5941 aataaataca aatctttaaa catttgtgtt cctccaagcg attttcagtg aattacagtc
     6001 aagtgccatt ttaccatatt tttggtaccg agctgcgttc ttatttcttt cttaattttc
     6061 tatgatacaa gattttctct aacgtttcta ttttacagaa gatgctagac aattgcaata
     6121 aaccaaaaaa ggctgcgacg gttgtcacag cctctctcgt attttgtgcc tatcaatgat
     6181 agcgttggat cctggaagaa tgagcgctat ctggttgttc ctcataagat gtatcatcat
     6241 aagtcatatc gtcatatgcc tcttccataa catagaattc ctccgcaact tctggttcat
     6301 catatcgatg aacggccata gccaacagga tataaataat cggaacccat gggtatgaag
     6361 tcaatgtatc ctccataaat gactgcatca tcaccgcaaa aaaggcccag ataaaagtca
     6421 tctgcccgtt ggataaatct gctttcaacg ttaagcgacc aataaaaagg ataagcagca
     6481 aagcagcaat cccaaatcct atcaatccat agatattcaa aatagaatta taactattgt
     6541 ggtttcctaa aaattggttt cctcgttgga agataaatgt tttcattcct agtaaaattt
     6601 gttcagattt ttctcctagc gtctgataga atttatgcca aatatcttct cgaccggtaa
     6661 acaaattgac tttttcagag acagccgcca gataagaaat cggcaaagct aaaagaatcg
     6721 taaccgtgta agcaataaat ggcgcacgaa tgacacgcat caacgattta ggtaaaagat
     6781 ccaatatcgc aaaaacaatc aacgcgacta atgcacctct agattggcag acccaaatcc
     6841 cgagaaaagc tgcaacaact gctactgcac cgatgattcg tacataccat cgttcaaaag
     6901 aactggcaaa tccactgatc aataaagcaa gagtcatcaa agaacttccg attatattcg
     6961 tattgatcca aatatcagaa agcgaattgc ctggagccca gatgctttgt ccttctggta
     7021 cttggatctc tgtaaataca cgatataacg tgattccgag gaagaccgaa aagacaatca
     7081 gataaatacc gcgatcccat tttgtccact cgatccattt aattactaaa caaaataaaa
     7141 taatcaaagg aaacagtaag tctccaaata aagactcatt tcttaattcc cgaaagtaag
     7201 aaaagaaaat gaaaagggca agcgcaacac ctagataaag aaaatcgacg ccttttgctt
     7261 tataaacatt taaaatgagc aaaatagcca cactaaaagc gataagtaat gaactattgt
     7321 tatatataaa caaagttgct ggaaaaccta accctgctgt ccaaacaaaa aagactagcg
     7381 tcagtaacaa aagcaagaaa ccgacactca ttttgttgac aaatttttgt acgattgctt
     7441 gaattatctc cactctcctt tctactaaca tttcaggatt attctcagct agcctattac
     7501 tgtacgacta gctgattttc ctgagtttgt gtttgtggta catctggata atccggatat
     7561 tctactttaa aaatcatata accgtcttta tccggtccat atagctgcgt gaactgcgct
     7621 tcatataatt ggttgtaatc agttaatggt cgtttactga tcacatagtc gatccctaat
     7681 ttttctgcgt cttcaaaatt caacgtgacc gaaaacatat ctggacgatc taaaatgaac
     7741 tttgtatctt ccttagcgat aaaagctctt gtatgagcat accgattata atacttctcg
     7801 tattcttctt tcggatcgat tttcttccat gcagctatat ctggataaaa gcgtactgta
     7861 ttgaaggatt tcactcccaa tgttggggtg aagttatata attcatcttc agacaaccaa
     7921 actttaccag gatcttgttg atcgattttt tggatttctt ttgccagtgt tttttcaaaa
     7981 atcgctcctg taccttctac gactggattg acgaaaaagc cagatacgat aactaaggcg
     8041 gcaaaagcga ggtaaaacaa gcgtctccag ccaaataatg caaaagctaa aatcactgtt
     8101 cccaacacag caactgcgat ggtttcaaaa tccgaaaaat aattatccat tttcgaatgc
     8161 gtaatcgcat agaaataaga ccatgcattt actgccaaca aaatgaattt ataccaaata
     8221 ggaataacat cttctctcca aatataagcg ataaaccaga tcgataataa acaagccgca
     8281 aaactaaaag taaggatggc tcttaatgga ggaacataag acagcagagt gatttctgct
     8341 agccattttg gcaacccaac ggtaatccaa aagatagaaa acagacaaaa aatcatcaaa
     8401 atacgtccca gtaacttttg ttcgctatct tttttgccaa ataacacaaa tggacttgct
     8461 aggaatacag ttggaaaaaa gtgatagaac agggccactt cagagttatt cgaatagttg
     8521 atatcttcaa aaggaatttt ccaatttgta aggaaagaaa aaaattcgcc aatcgtccac
     8581 tttcctccag tacttacccg ttttcctgga tataaggtat tcaaagaagc taacaatgcg
     8641 tcttttgacg ttagccagaa atgaaccatg actccgccaa taaataaaac tgcccctcca
     8701 atcaacacta catccaacag atcccatgaa actttcttgc tatagtaaat aagcaggcca
     8761 acaaaataaa aaatcagcaa gtacgccagc atcacttgat gggctgggta aatcactaaa
     8821 ataaacccaa taccgaaaat aaccaccaaa agcatcatca acgttcgtag ccatttttta
     8881 tcatgattgt agaaataatg ataaaaagca gtcatcagtc ccaaagtaaa gaaaatcaaa
     8941 tcgcccacat gttgcatgaa ccaccactgg actgctggag aaaatgtcag cgcaaatgca
     9001 ccgattagcg atagagcttt ttttctcttc gttaatatca tcagtacttc gaatccaagc
     9061 aagaccatga cgactaattt catcgcccaa taaaaggaaa gtcctcgatc tcttccgagg
     9121 aaaaagaatc cccagttgaa tggttttccg ataaccgtaa tatcctttac aggagaatta
     9181 tacgcaatga tcatgttttg tccgtttaat gaataatcct cgttcaccaa cggaaagtcc
     9241 tcttcagctt gtgagaggta aaaaggtgtc tgcaccagcc attcgtctga ccgcacttcc
     9301 cgattctgtc caaaaatcac atcagttttt ttcccatcgt ctctttgcga cacatattta
     9361 tcccaaacac caagtgaact tccgtttaaa ttaaaagtaa cgatgaccac gatgactaaa
     9421 acgccaataa gatagcggag ttttagaaac aggtcaagca ccaaattcga atctgcctgg
     9481 ttcgcttttg gcttttcttt tcgtcgtgca cttcttgttt gtttttccat atcatgctcc
     9541 aaattctatt tgataaaagg aaattactta ttcatttttt tggcagcttc cagcatttgc
     9601 gcaagtgctt cttgccaagt tggtatgaca aatcctaact cttctgtctt tttcaggctc
     9661 atgactgaat actgcggacg tgttgctttt tgcggaaatt cttctgacgt aacaggtgca
     9721 acttctacat ctgtatcttt caatatttct tttgcgaatt cataccaaga acagctattt
     9781 tcgttcgaca aatgataaat accaaatggt gctttttctt cgatgacata taccataaat
     9841 tctgccaaag tacgtgtcca agttggtcgc ccaaactggt catttactac agtcagtttg
     9901 tcgcgggttt ccgctaggcg ttgcatcgtg aagacgaaat tgtgtccata gatcccgaaa
     9961 acccaagaag ttcgtatgat ataatagtct tctaaaatct cttctactgc tttttcgcct
    10021 aataatttcg tacgaccata ttcatttagt ggattcgttt ggtcgtctac ttcgtattct
    10081 acatttttgt tttttccatc gaaaacatag tctgtactga tatagacgaa ttgagcattc
    10141 acagcttttg ctgcaagtgc tacatttctt gtaccatcaa cattgatctt ctcatctaat
    10201 tcttttgctt cttcttctgc tttatctact gctgtgtaag ctgcacaatg gaaaataaca
    10261 tcaggtttta attctgtgat gtaagccatt gttttctcgg catctgtgat atccaagtct
    10321 tttgcatctg ttgaaacata ttgcagattt ttttcatcta ataaatggcg caattctgta
    10381 cctagttgcc catttcctcc agttaataaa aacatatgat tatccgctcc ttgattttta
    10441 ttttaaaaaa gagacaggcg agcgcctgcc tcacatgtgt ttttccatta atacagtgac
    10501 attttctctg tattattgtc catttttggc atatgctgct tcaacggcct ctttttcttt
    10561 tttccaccag tcttcatgtt cttcgtacca tttgatcgtg tcagccaacc catcacggaa
    10621 gttcgtaaac tcaggcttcc atcctaactc ttcccgtaat tttgtcgaat cgattgcata
    10681 acgcaagtcg tgtcctgcac ggtcgtttac atggtcgtac gcatcttttg gttgacccat
    10741 catttctaag atcaattcca tcacggtctt attatcttct tcaccgtctg caccgatcag
    10801 ataggtttcg ccaattctgc ctttcgtcaa aatcagccac actgctgaag aatgatcatt
    10861 cgtatggatc cagtcacgaa cattctttcc ttcgccatat aattttggac gaataccgct
    10921 taagatattc gttacttggc gaggaataaa tttttcaata tgctgataag gaccatagtt
    10981 gtttgaacag tttgaaatgg ttgcacgtaa gccaaaagaa cgcacccatg cttttactaa
    11041 cagatctgaa ccagcttttg tagaagaata tggacttgaa ggattatatg gtgtttccgc
    11101 agtgaatttt tctccttcgc cttccccatg tcctggcagg tcttcacgta atggaagatc
    11161 gccgtaaacc tcgtcagtag atacgtgatg atagcgtaca tcatacttgc ggcaagcttc
    11221 taaaagtgta tacgtaccaa tgatattcgt ttgtacaaat gggaaaggat cttttaatga
    11281 attatcgtta tgggattctg ccgcgtaatg aacaactgcg tccgcttctt ttactagacg
    11341 gtccactaat tctgcatctg caacatctcc tacgaccaat tctacgcgat cgctaggaag
    11401 tccagccaag ttctctttgt ttcctgcata agtcagttta tccaaaacag tgacatgcac
    11461 ttctggatga ttgtttacca cgtaatgaac gaaattagag ccgatgaatc cggcacctcc
    11521 ggtaacaatg atgtttttca taagaaaatt tctccttaat tcttagattt ctccgtaaac
    11581 aaatggattt tctgcttcaa attcttttaa tgtcggatga cggtgatctt tttccgacat
    11641 aatcgctctt gatggatcaa ttggccaatc aatcgctaaa tctggatcat caaatgcgat
    11701 gcccccatct gcttctgcat tatagtaatt atcacactta taaaggaagt tcacatttgg
    11761 tgtcaaagta acgaatccat gggcatatcc tttaggtaca agcaactgtc gatgattatg
    11821 ttcagataag ataaatccgt cccattgacc ataagtcggg ctacctttgc ggatatccac
    11881 tactacatct aaaactgctc cagtgactac ccgaatcaat tttgtctgtg cagcttctcc
    11941 tttttggaaa tgaagccccc gaaggacacc agcttcagct gatagtgaat ggttgtcttg
    12001 aacaaagtca tatttcaatc catgttctgc aaattttgct tttgaataac tttctgtgaa
    12061 gaaaccgcga tgatcgccaa agacgtccat ttctacgatt ttgacatctg ttaattttgt
    12121 atctgttact ttcatctgta aggactcctt attcagcggc caaattcaat aaatattgac
    12181 cgtattgatt ttttttcaat ggctgtgcaa gggccagcaa ttgttcctta tcgatgtagc
    12241 ccatacggta agcaatttct tccaaacaag ccacttttaa gttctgacgt ttttcaattg
    12301 tttcgataaa ggtagatgct tcaagcaatg attcatgtgt tcctgtatca agccaagcaa
    12361 atccgcgtcc cataagttct acggatagtt ttcctttatc caaatatgct ttattgacat
    12421 ccgtaatctc tagttctcca cgttctgaag gtttgatacc tttagcgata gaaattacct
    12481 cattgtcata aaaataaagg ccagttacag cgtaatttga ttttggctga gcaggctttt
    12541 cttcaatcga caaagcttgc atattctcat caaattctac tacaccaaag cgttctggat
    12601 cattgacatg atagccaaat actgtcgcgc ctgtatcttt tgaagctgct cgttgcaaca
    12661 ttttagacag accgccacca tagtaaatat tgtcccctaa taccaagcag acactatcat
    12721 ctccaataaa ttcttctcca atgataaacg cttgtgccaa tccatctgga ctttcttgta
    12781 ctgcatattc gatctgcagt cctaaatcat ttccgtttcc aaataattcc ttgaaacgtg
    12841 gtgtatcttg tggcgttgaa ataatcagaa tttctttgat tcccgccaac atcagagtag
    12901 acattggata ataaatcatt ggtttgtcat aaatcggcat caattgtttc gatgttgcct
    12961 ttgttaaagg gtacagacgt gttccgctgc ccccagcaag aatgattccc ttcatggata
    13021 tctgctctcc tttttgattt caaatttcac tttgttatta tctcatcaat tgttatattt
    13081 gtcatggaaa tattaaggat ttaaacatct tttatcaacg tttaaaccat tttcgtttga
    13141 tcaacgccgt aatcagatga acacgccaag aaattttttg ccgtaattta gaatgtgttt
    13201 caatctcact cgcgtttaaa tcatgtcttc ggtacagagt taacggttct cttaggaacc
    13261 cagtcttctt tcctcccagc aaaccaatcc acatatcatg catcggcact tctgacggga
    13321 tcggcaaaat atcgggtttt aaagaagacc ggaaagccat accagcacca atatagtgac
    13381 ttcttatcac attattaagc catccttctt tggaatttcg aaacgaaaaa tacgaatcat
    13441 ggatctcttg cagctcgctg tccacaatga ttaaatcaga aactactact agctgatccg
    13501 gattctgctc aaaatagtca gtgatctttt tcacttttgt ctccaaccaa acatcatctt
    13561 ggtctgccaa aaagattaga tctcctttcg tctgctggat cgcataacca aaattagcga
    13621 tcaatccttg acgaggcccc gaaaaaagct ggattcgctt atctttttct gcgtattcag
    13681 ttaatatatc ccatgtatca tctactgacc catcatcaga aatgactact tcatcttctg
    13741 ctgaaagctg agataaaata ctatctaact gctctcttaa atatctgcct ccattaaagg
    13801 tagctataca aaccgaacgc acgaattcac accttccaat tcttaacata tgagcgcaaa
    13861 gtcatccgcc agatatgccg gtttttgacg gtcaagaact gtttagctgt tcttaacaat
    13921 aattgtcgtc ggtgactgga taaatgcctt tgatcatatt catgataaaa tcgattttct
    13981 gcttgtaaaa tagaacggta tcgattactt gaaacttttt catagtctaa aaccgaaaga
    14041 tcatgttcca ttcgtacagg aaggatctcc acagtttttt ccaactgatc cactttccaa
    14101 aacagccaat gatccaaaaa atctaaagaa aaagcaggat taaacccgcc aatctttttt
    14161 agaaatttga cagaccaggc aacacctgaa ttgattgcca tgattcgttc agctgttggg
    14221 ccttcaactt ctactgcttg agctgtacga ttgatataac gatctgaata taggggagaa
    14281 acttgtcggc tcccagaaaa aatcatcgga actgcagcaa cacattcatc tgtaaaggtt
    14341 actttacgca atagttcaaa ataatcgtcg actagcctcg tatcctgatc cagagtgacg
    14401 agacaacgtg ttccttcatc tgcttgagac aacgcaaaat tataagccac cgctaaacca
    14461 ggattttgcg gatcatgatg ataaacgata ttttcttttt caaacagctc attttgctgc
    14521 ctttgaggac tattatcgta aataataagc tgaatttgct tttcgctaag tgctttgctt
    14581 aatggtccat aagctggaga ttgactgaat agttgctgat aaagcacaac gactactaat
    14641 atttctggtt tcatcagaaa aaacgccacc cccacttatt gaaaaatttc atcatagaag
    14701 tcaaaaacac gcgaaacagt tttgcatttc gatgcgcacc cttcccgtaa aaatggataa
    14761 ctgcataaaa gggtgtgtac aataacttgt aacctgcttt ttcaaaactc aaacacaagt
    14821 cgttgtcttc aaagtacatg aagaaacgct catcaaaccc ctttatctct ttaaatttat
    14881 caatatcagc taccataaaa ctacctgaga tcatccgtac ataactatta gcatcagtag
    14941 gcaatcctcg gcattcaaac acagccagtc gtttgtcaaa cttttttttg acccatttga
    15001 aaggcacaaa gcgcaaaacg taatcaaaaa cagccagctg ctcacggatg aggtgctgag
    15061 ttgttccatc ttcattaaga atcttaggtg ctaacatcgc acattccgga tgttctttca
    15121 ataacaagac caatgctgta actgtctctt catccactaa aatatctggg ttacaaataa
    15181 tgccatagcg agaatcgcta gctatcagat tgtgattatg tccataacca aatccggaat
    15241 tttcatcatg aaaatagata tcgatgtagg gatatttcct atactctgtt agtctttttt
    15301 tgtactcggg aagtgaattg ttgtcaaaaa ttcggatttt tactgctgtt tcatctttta
    15361 cagttgaaat aaaattatct agtgtccgaa aaattttatc actgtttgcc gtcacaatac
    15421 taataaggat cggctggttt aatttgttat tcatctatat gacggttcca tttcttcaaa
    15481 tatttctgtt ttacttgcgt acggtactct ttattcccta aaaatcgcaa tgtttttaaa
    15541 ataaactgat gtttctcatt gatcagacca atcaattcaa ataataaaat tgcagcaaac
    15601 aacatggcaa taatcaatat gatcgttccg attcttccag tataattcat gaccatagct
    15661 gtgaaagaaa acatcaaagc catcgcataa atcgtcagga cagcaccttt atgagtaaag
    15721 cctaaactga ttagtcggtg atgaagatgt tttttatctg ctgtagaaaa agaaacacgg
    15781 tttgcttttc tcctaatgat agcaaaaaac gtgtctgtta ccggcactcc catgacgatc
    15841 agcaaagaaa tcgatgaaac aaaagtcacg ttcttcagcc cttgtagtga aaggacggcc
    15901 atcataaacc ctaggaatag agctcctgta tcgcctaaat aaatctttgc cggataaaaa
    15961 ttgtaaggaa aaaaaccgat aatacttgct acaagacaga aaataacaac tggaatatac
    16021 actgtagaag catggaggaa aaaatatcca atgattccta tcgtaattaa accgatcaat
    16081 gaaatccctg ccgccagccc atccaaaccg tcaatcagat tgaccgcatt cgtgataccg
    16141 aagatccaaa aaatcgttac gggaaaacta agccagccta gttggatata tccaataaaa
    16201 ggaagcgtga aggaatcaat acgtatatct gctacaaaat aaataataag agaagcaatc
    16261 aagatcccta tacttttttg tttaggtgtc agctcatatt tatcatcgag cactcctgtg
    16321 actatgatta ctaaagaagc aatcaagatc ggtacaatat acgattgagg gataatttta
    16381 cggaataaga ataaagtcga tataacaaat gaaataaaga taggcagtcc gcccgctgaa
    16441 ggcattggaa ctttatttat tcttctttca tttggtttgt cgaccatacc tgtttgtact
    16501 gaaatgattt tgaatacagg tgttaatatc agtgataata aaattgtcag aaaaaatctt
    16561 ataaccatac taaaagaaac aagcatatgg gcacctactt taattaagag tcataattat
    16621 tataaattat atatgtcttt ttgactatct ttttatatct tctcaaatgt ccgcaaagcc
    16681 ttattttatc ggctctacgg gcattttgct tttgtggtaa acctcgcata tctaggtcta
    16741 tcttcttata ttttccctgt caatcgtggt taaaatcgtg gtaaatactt ctatgcgatt
    16801 agacgctgaa cctctgattt tgcggtatct atggacgcat gagcgtacca gttcattgtg
    16861 atactgatgt ttgaatgccc catgatatac tgtaaatctt tcgggttcat gttcttgctt
    16921 gccagccttg tgcagaatgt atggcgtagc gtatgcggtg taatatgtgg caagggattg
    16981 tccttgtggt gcttgttgta tttctttacc atacggataa ataaggcgtt ataatcaatg
    17041 gcaacttttg gattaccttt cggattgata aacacaaaac cgctgtgtcc gtctacctct
    17101 atcgacttta tctttggtcg gctgttgata acccgttgaa atgcctgtat cgtttctttg
    17161 cttaatggca cttgccttat cccgcttttc gtcttaggcg tttcaatata atagccctgt
    17221 tccttactct ttagtaactg gtggtcgata atcacaactt cattcttgaa atcaatgtcg
    17281 gctactgtca gtccgcacag ttccgagata cgaagtcctg tctttaacag tatcagcaca
    17341 tcatcataat gcttgtgata cacgttgtcc gtcttaataa atgacaataa ggcttgctcc
    17401 tgttcctctg tcaatgcgac tttctctttg gtatcatttt ctatgacttc acttagcttg
    17461 aaatcaaaag ggtttttcct cacataatca tcttgtatgg ctatgtagaa tgacgctttt
    17521 aacgagcgtt tatggttgtt aatggtattg taggaaaagc ctttgtcttt catgcgtaac
    17581 gcccattctt tagcgtcaga gggttttatc gtatcaatgc tcctagcacc taacttgtcc
    17641 tcttttaata accgcatgag ttgttcccgt tgtttctgtg tgcttttctt cacatttgtc
    17701 ctctgtgcgt tctgtttggc atagagttgg caaagcgtca ttttcttgcc tgtgctgtcg
    17761 ataccgtcct caatatcccg tcttatctgc tgttccagtt ctcgaagtga gggtttttcc
    17821 cgttttccct ttggtgtcgg gtctgtgggt gtcaatctcc aagcatacac atattttgtg
    17881 tttcccaatg cgtctacata tttatataag tattttccgt ctgttcgttg gctctctcca
    17941 gtatgcagga tacgaccttt gttatcccgt cttttttctt tcatggtgtc tgctcctttc
    18001 cttgttggaa agagccttga tatgacttgt gttcatcata acacatacaa ggctcatttg
    18061 cattagattg cgtccaattt atcaataacc ttttcaaact gtcggcgttt aatctgtatg
    18121 cggttgccat tcataatgag ccagccagcg tccttgtttt cctctgccaa tcgtcttaac
    18181 ttgttttctc caatgcggaa atactttgac gcttcttcaa tggtaagggt gtatttttcc
    18241 catacgggaa tatcctgtgt attactcata aaacaccccc tcactttcga ttacgtctga
    18301 tatgtccgta cacatctgtt ccacaagcat ttcctgtttt tcagaaaggt gggtacattc
    18361 atcaatcatt ttcatgtagt ttgaacctgt cagcctgtcg atttcctcta tcatcacgat
    18421 tgacggcatg acctgtcttt ccagccattg cagagtgcgt tccatattga caggtacagg
    18481 gttcatttca aaaggtaaac tgtcatggga aatgaatatc tcccacaatg gatagtcagg
    18541 aaattccaca aaatcggtaa tgagataaaa caccagccca tgagggttat acgtcaataa
    18601 cagttcttca actgcctgta ccgccttttt gtcacgcaga cgaatttcaa aacggttgat
    18661 aatatcggta tgtttctttt tcgttgcctg ttccttttgt ttttcataca ggcagaaata
    18721 ttttgtgctt gcctttgagc cgatataaag ggtacttgct aaattacggt tttttccgct
    18781 taattttcca ccttgtacat tttcatagtt tttacagcga cattccgcac caccatttct
    18841 gtatttttca gatagcacag gaatatccag cagaccgcac atatcattga ttgccaagtc
    18901 aaaacgtcgg ataacaccgc cacagtcaag acagcggttt aagaatgaat accagtccct
    18961 gttctgtgcc tgtaagacat attccatgtt gcgtgagcct gcccctttta attctaaaaa
    19021 tacgcccatg tgttcatcat cagaagccat gaccttgatt tccccgtaag cataatgctc
    19081 cttatagcca taatacccat agtcataatg gataaaataa tctgctttca tgccaagcac
    19141 ctttcggata acttccaatg cgtcagtcgt tggaaaacgg acagacagat aatcaacgag
    19201 cagataaaaa ggcttatcgc agatatagta attcagacaa cgcaggataa tgttctgtgt
    19261 ttccttgctg gcgttagctt ttccgttctc aaagcggttg atactatatc ttgatacatg
    19321 ggtaagactt gcaagttctc cttgtgaaag accgctggca agacgtttgt tcttcatttc
    19381 aagtgcaaat tctaaattgt tcattgtatt cctccaatcc cacaatagcc ttgaaatacc
    19441 tgtaaaccac ctttcaaaaa gtgtgatatg tactatcttc ttaaaacctc tcattactac
    19501 tcacaggttt gggggtgtat ggtttttgac ttgtcttttg tgaaatagtg cccccctgtt
    19561 agataacggg ggcacagaca aggctcgcag gctcgccagc cgacaaagca tttcttcccc
    19621 ttgacacaag gtgtcatggc ggtaagcctg agaggcttcc gccaccgtgt cagaaatact
    19681 ttgtcagcaa gttctgtttt gccttttcta acaagttcca tttcaaccga tattcatatc
    19741 tacggattta atcagatagg tatttctttt tgaatgtttt tatatttcat tctattgtgc
    19801 ttattcgatt acaagaacat agtaattctt atatgcttat ttgtcaatat ctttttttgc
    19861 ctttacaaat catattaagc atgatagact tataatagcg aagatataac gagatatgga
    19921 gggttatgca atgaaaataa gccatttagg aaataatata cagaccataa gaaaatttag
    19981 gggaatgaaa caacaggaac ttgcggacaa aatcggtatc aatatgcaga gcctttccaa
    20041 gattgaaaga gggttgaact atcctgccta tgaaacgctg gaaaagataa tggaagtgct
    20101 ggacgttacg cccaatgaat tgttgtcggg agaatggaag tatgtcaatc aatctgaaaa
    20161 ggaagtctgt cagtttttaa gagccgaaga acgtctaaat gcagaactga aacatggaca
    20221 ctatgataac ttctttgaca gcgaggaaga atggctggaa tatgagttag aaaagttacg
    20281 ggaatatatt acagactata tcaacgggaa aagcattgaa gcgtccgacc tttacccaat
    20341 caaagaattt atccagcatt tgaagtttca aaaattatta gaccgctatg atgatttata
    20401 cagtatggat atgtttgggg aaagcataga gggacataag tataagacac cttatcaagt
    20461 cgtgaaaatg ataaacccga actcaaaaga agatatggaa ctgttacgag aagtattaag
    20521 gaataacaat tttgatgatg aggacgagta acttttttcc tcttgcgaga agatacagca
    20581 aacaaaaagc ccgatcaaga gtgcaaagca ccaccaatgg tggcaaagct cttgacaggg
    20641 ctttttcttt tctgttgtgg gcaatcaaga ggaagaaagg gataaaatct gatttgtggt
    20701 aaatcaggca aaggcaacgc aggtatcaca gtatgaattg acaccgcata aggaaagcaa
    20761 ctggaagatt gtagaataaa tgaaaaaggc ttgcattctg catttttata aaattgcaag
    20821 tctttatatt gacaagcata aaatactaag atatacttta atcagaccaa cggtttgtat
    20881 tggaggtaag ttatggcacg aaataaatat ccagaagaaa cagtaaaact cattttagat
    20941 gtttctacaa aaatgttttg tgaaaaagga tatgataaca catcattgca agatattatc
    21001 aatgaaacaa aactttctaa aggtgctatt tatcatcatt tcgattcaaa ggaagatatt
    21061 ttaaaagcaa tttttcaaag aattggaaat gaaaatgcag taatatttgc taaaattaga
    21121 gataacgata gtttaaacgg tttagaaaag ttaagagaaa tatttaagac agcggtattc
    21181 aattctaacc aaagcgtttt attgacagtt tctcctcaac tattaaacaa tcctcgtttt
    21241 cttgctatgc aaatagaaca aatgtatgaa attgtagtac ctgaatttgt tgaacccgtt
    21301 ttagaacaag gtatcaagga taagtctttg gagattgaaa attcacatga aattgcagaa
    21361 gttatcatgg tattaagtaa tgtatggctc aacccattag ttgaaatgac ggataaagag
    21421 ggtatggaaa aacgttgcaa tacattcaat gcccttttaa atggaatagg catagacaca
    21481 ttattagacg aagaaacaat ttctgcattt atacagtttt gtaaataact gtatttgtta
    21541 tccctatacg atagactgct ctataaaaga gcagtttatt taaaccatta tataaaccga
    21601 cggtctgttt gattaaataa aaaggagaga ttttatgcaa gcagaaaaaa aactttttaa
    21661 tcgtgatttt atacttgttg ttataggaca gataatttct ttatttggaa acgctatcct
    21721 acgatttgca ttaccgtact atttgttaaa agaaactggt tcatcagctt tgtttggtat
    21781 tgctacagcc tgctcttttt tacctatggt tattttgtcg ctggtaggtg gtattattgc
    21841 tgacaggaca aataaaagaa acattatggt catactagat tttgtaacag caatcataat
    21901 tttagtattt tatttgtcat tgggaaaaat tcctatcata cctttgttta ttgttgcttt
    21961 gatgttactt tatggtattt ctggtgcata tcaaccagcg gtacaagcaa gtattcctct
    22021 attggtatca tcagataaac ttaccgtcgg taatgctata ataaatcaag taaatacttt
    22081 agcaaacttt atcggaccag ctataagcgg agtagttttt caattttatg gtatcaatcc
    22141 cattttaatt gttagtatta tatgttttac tttttcagcc attatggaaa tttttatcca
    22201 tattccacac caaaaaagac ctcgtgaagt aggtgtaatt gcagttgcta aaaacgattt
    22261 aaaagatagt tatacctttt tgaaaaagga gaaacccatt tttatatccg taatcttttt
    22321 ggtttgcatt ttcaatttgg ttttaagtgc tgttttaatt gttggtctgc ctatcttaat
    22381 ttctgaagtt ttaaaaatgc cagatacact atatggtttt tcacaatcag cattatcatt
    22441 aggtggttta tgtggtggta ttttaacagc tcttgttgct tcaaagttaa aactaaaaaa
    22501 cctttatacc ttgttactta tttgttctgt ttctgtttta cttatgggaa tttcgctatt
    22561 tttagaattg cctgcaatag tttcctactg ggtagttaca gtgatgtgct ttgtggctat
    22621 gggtgtatct actatgtttg ttgttcaaat ttacactctt gttcagacac aaacaccacc
    22681 gcaattagta ggaaagataa tggctacgct ggtagcagtt gctatgtgcg gtcaacctat
    22741 tggacaaatg gtttacggta ttttattcga tgtatttata caagaaacat gggttgttat
    22801 gtttgtagct tctattgctt caatcatgat ttcaatatat tcaaaaagaa tttttcaaaa
    22861 gttagagata taatacaagg ggttaaatag tatgttaggc taaaaacaat atataaaaga
    22921 aaatttgaaa tctgctaaaa ggatagtgtt ataaaagaaa gagtaatatt gcagtattta
    22981 aggctcgcac aaatatatgt tttgtgtggg cttttttctt accatttttc tgtatttcta
    23041 aagggtataa acggtattcc gtttatatat ttcagcatta gcagaaaggg ggtgagatta
    23101 tgaggacatc ttctttcgag catatcgtca gattacagtt caattcttta atgctgattg
    23161 tcattaagaa caaagtgaaa agtcgaaaca gacagcttgc aagacattct aaacgtgaag
    23221 tattgttttg tgaaatagcc gaaacgaaac aagttgaacg tggaacgatt gatacctgtt
    23281 tttatgattg tgtatcattt aaagtcttgc actttacaat ttatgtatct gatgaaacac
    23341 ttggtacagt tttacataga ctgtcagaaa aacagcgtag tgcgatttta ttacgttatt
    23401 ttcaaggaat gaacgacaga gaaatttcag aactgtacca tgtatcacgt tctgctattt
    23461 gcagtagaag aactagagga ctaaggaagt taaaaatgct tttgagcgaa aggaagtaaa
    23521 gaaatgagta aagattatgg ctatccctct tttgaattga tttgcaaagc gtcaagcgga
    23581 gatgaaatgg cagttaaaga gattttgaag ttttatgacg gttatatttc taaattatgc
    23641 ttgcgtcctt tttaccactc tgaaagtggt aaaatcatta tgcaggttga tgaagaacta
    23701 aaaggagaaa ttcatacaga catgatgaaa gcaatattga aatttgaaat cggagtgaag
    23761 taatcaactg tataaaaaaa tcgggagata cacagtttcg tgctatctcc cttagtagtt
    23821 ttacggtcat atcaaggctc attcaatcgt ggtaaattcg tggtaagatg agtgtttttc
    23881 tatacttcaa aatgcttgaa tgtatagtgt ttatgcggat aattgaaaat tagatataaa
    23941 atttcaaata aattactgat ataaatttac aataagaact cttgtttcta ctttttttta
    24001 tgagaaaatt tagttttcaa tttattgaac cattttcggc ttggatctcg acgttttttg
    24061 atttgatgac ccgtcacgta ttcttgaaca actgcattga tgattccgcc caaaatgatg
    24121 atcgtagcag caaaattaag ccaaagcatg agtacaataa aacttcctat gatctgataa
    24181 ccgctgatcc tcgttgcaaa atacctagca tagatactaa acacttgtga taacagcatc
    24241 cagccgacag tagcgaagat cgtgcccgga atgatcgtct tcaacttcaa ctgagcatta
    24301 gggacaatta aataaatcaa gaacatgatg acaaataaag ctaccaatgt gattggccac
    24361 ttcaaagttt ggaactgatc gataaagttc acgggtattt gtacgattgg ctgaatagca
    24421 tccaaaatca gctgtcccaa cccaagaacg atagttaccc cactaatagc aatcatgaac
    24481 aagacaatga caattagtga gaagaaacgg acgatcaaaa aattttttct attttctacg
    24541 ccatacgctt tattcatcgc aatttgcaaa gcattgatac tttgacttgc agaccataaa
    24601 gccgctaatg cagaaacgga taacaagcct cctgaacttt gtgtcaaaag gtctttgatt
    24661 gcaggaccga taaactggta aacggtctca ggaatcactt ctcgtaaata ggtcagtaca
    24721 gtctcttgat cgatgtggag aaacggtaaa atattaccaa aagcaataat caatggaaat
    24781 aaagacaaga gtaaataata agcaacgacg acagaggtcg tactcatttc tgaatcagtg
    24841 atgcgctttt gagtcgtctc aataaagcgc atcagatttt cattttgttt tattttgttc
    24901 caaactttca tgtacctctc ccgtacctat tgactggata gatcaataag tgccttcttc
    24961 tccttgtgat gtcaaaattc ttggaccatc tttagtaata gccaatgtat gttcatattg
    25021 gcaagaaagt ccgccgtctc ttgtatacgc agtccagccg ttaggatcca ttttcatttt
    25081 ccaagttccg gtattaacca taggttcgat cgtgataacc atgccttctt tcaaccgcaa
    25141 cccttttcca gcttctccgt aatgaggaac tgctggttct tcatgaatcg taggaccaac
    25201 gccatgacca ataaaatcac gaacgatcgc tagattttcg ccttctacat aagtttggat
    25261 agcatgaccg atgtcaccaa tccgattccc tacttgtgct tgctcaatac cgatgtacaa
    25321 agctttcttc gttacttcca tcaatcggtc gatctcaggt gtagattctc caactacata
    25381 ggcccaacaa gaatcagaaa tgccgccttt caaatcgata cacatatcga cttttaccaa
    25441 atcaccgttt ttcaaaggct ttttacgagg gaatccatga caaatttcat catttatact
    25501 gatacaagtc gcatattcat acccttcaaa gccaatttgc gcagcaattc cgccatgact
    25561 ctcgatatag tttctgacaa atacctcaat atcccaactt gtgattcctg gttttataaa
    25621 atcacgcaga tttttatgta catctgctaa taaagctcca gattctgcca tcatttcaat
    25681 ttcacgtgct gattttaaag taatcatatt ttcgtctcct ttttacaaat ccctctcatt
    25741 ttaacatagc ggcatacatt tagccaaatg acattcccca ggtgtctcta ggttttattt
    25801 tcttttcaat gatgttttgg tataattcat aattgttaaa taattataaa cgaggaggca
    25861 catcatggct ttagcaaaaa tcgtatatgc tagcatgaca ggaaacactg aagaaatcgc
    25921 cgatattgtt gcagaagcat ttgaaaatct ggatatcgaa gtcgaaatca acgaatgtac
    25981 ccaagtagat gcagatgaat tcgaggaggc agatatttgc gtagtagcta cttatacata
    26041 cgatgatggc gatctgcctg atgagatcgt agacttttat gaagacttgc aagaattgga
    26101 tctattggga aaaatttacg gtgtctgcgg atctggtgat actttttacg atgaattctg
    26161 caaatcggtg gatgatttcg atgctgcttt tgcaaaaaca ggcgcatcaa aaggagccga
    26221 aaatgttaaa gtggatctga atgctgaaga agaagatatc gaaaatttag aagctttcgc
    26281 taaagaacta gtcgcaaaaa tttaatatac ttgcaataaa agagccatcc agatttattc
    26341 aatctgtatg gctcttttat tcattgcatt catggaagag ttcttcggga gttaaaaatg
    26401 catacgctgt aagaaaaacg aacttttcct tattttgaca catctcttgt tacctactga
    26461 tttgtggaac ttttgagtat attcaatcga tttcgattgg ttctttgaat ttcaagatag
    26521 catctatact gtcttggatc atcttttcaa tctgttcatc agttggatgg atgatgcttg
    26581 aagacatcaa agctgcgatc ccagtagcta tgatccatgt cccattatac aaagattcga
    26641 tctgctcttc tgaaagattc acataagctg gatcgcgttt cactgtattc acaaagtaag
    26701 tataagagaa attctgcatc tcttttcctc caccatattc ttctaagtac agtgaacgat
    26761 acaacttctt ctctcttctt gcaaaatgga tataattaaa agcaagatcg ataatcgtat
    26821 tccctgtatg ctcaacagtg aacacttcat attgtaaatg atcatagatc tgctcaaaaa
    26881 gagcttgtct caagtcatcc atatttttaa actcaagata aatcggttgc gtggaacaat
    26941 tcattttgtt cgcgatgttt ctcgcagtaa atcgtgtaaa tccatcggtg gcagctactt
    27001 cataagcagc tgccaaaatc tgttctctag taatcgtctt ttttcttgcc atatttttta
    27061 atctccttaa tttttttata acgcttccac cattgattaa tttcattgta acatgttttt
    27121 tttgatatgc aaaattagtt tttgattttc ttcacaagct gaaatagcaa gtagtaacat
    27181 atatcatgaa acttaacctt aaaaaaataa cctccttttt ttatttaaag cctcattgtt
    27241 tgaatcataa ttaaatggta tcatgctaat agttaccaga aaaaatggtg aggaggaatt
    27301 tttattatgt tatctgattt taatgaactt ctaaaaaaat atgcacgctt aattgcagaa
    27361 actggcgtag ccacagaaaa aggacacaca gttgttttac agatcagcgt ggaacaggca
    27421 ccccttgctc gattgatcac acaagaagct tataaactag gagccgctga ggttatcgtt
    27481 caatggactg acgaccagat ccaaagagaa tttcttttac atgctgcaac ggatcgtatt
    27541 gagaatgtac cacaatctaa aatcgatcaa gcggatgaat ggcttgaaaa aggagcaagc
    27601 aggatcagcg ttgtttctgc cgatcccgat gcatttgctg gtgtagatag ccatcgagta
    27661 gcgtcttatc aatcagcgaa tggcaaagca ctaatgaatt tacgcaaggc aactcaagca
    27721 aataaagtca gttggactgt cgtggctgcg gctggtaaac aatgggcagc aaaagtcttc
    27781 ccagaactgc cagaagaaaa acaagttgag gcattatggg atcagatttt caaaacgact
    27841 cgtgtctatg aagaagaccc tgtcttagca tggaagaagc atgatgaaaa attagcaaaa
    27901 aaagcagaag aattgaatcg agaacaattt tctgcccttc actatactgc tcccggtaca
    27961 gatatcatta tcggattgcc taaaaaccat ctttgggaag gcgctggaag ctataatgct
    28021 cgtggagaaa aattcatggc aaatatgccg acggaagaag tgttcacagc acctgacagt
    28081 catcgagtag atggttacat ttcaagtaca aagccactga gttatgctgg caccatcatc
    28141 tcgggtatga aatttacttt taaagatgga aaagttgttg atttttctgc cgaacaagga
    28201 gaagatgtct tagcaaaact gcttgataca gatgaaggtg cgcgtcgttt aggagaagtc
    28261 gcacttgtac ctgacccttc acctatttct caatctggca tcatcttttt caatacatta
    28321 tttgatgaaa acgcatcgaa tcacttagca ctaggttctg cttatgcctt cagtgtgaaa
    28381 ggcggtacag aaatgtccga tgaagaatta gcagaagctg gattgaatag aagtcaaacc
    28441 cacgtagatt tcatggtagg atctgacaag atggatatcg acggaatccg tgaagatggc
    28501 tctacagtac cgattttccg aaatggcgat tgggcttgag tatactttcg cacacatgca
    28561 aaaaataatg gctctttgtc aataaggact gatgagttgt gcaaaatc
//
LOCUS       DASHHB010000207        20985 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.048, whole genome shotgun sequence.
ACCESSION   DASHHB010000207 DASHHB010000000
VERSION     DASHHB010000207.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 20985)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20985)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20985
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.048"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(134..577)
                     /locus_tag="U2K71_002995"
     CDS             complement(134..577)
                     /locus_tag="U2K71_002995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF305 domain-containing protein"
                     /protein_id="HEM7316984.1"
                     /translation="MKKYWKFLLMIGVSTVVMFGMMYLNVYALDHIFFSRTRVFMALM
                     MGAVMAVIMLLFMWKMYENKKINVMILAASLLVFAGSLFMVRSQTTVNDTAWMKAMIP
                     HHSIAILASKRADLKDPEVKKLAEEIIQAQEEEIDKMKKMIKEME"
     gene            complement(795..2135)
                     /locus_tag="U2K71_002996"
     CDS             complement(795..2135)
                     /locus_tag="U2K71_002996"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002331624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7316985.1"
                     /translation="MEDKKLKKQLSSRHITMLALGGAIGAGLFKGSGEAIGIAGPSVL
                     IAFLIGGLILYIVMKGLGKIVLSGGDTHHGLSGLIRPYLGAHSADFTDWVYWSMWIIN
                     IIAEAVAAASFLQLWFPHIPAWVFVFMLAVLTTIINLYSVRLFAETEYWLAFAKISVI
                     ILLIIFATYLVGQQMLGTGVFPTLQQLTDHGGFTPHGMKGIISSLLVVIYSYGGSELI
                     AITVSEADDPKRAIPKAIKGVMGRIISFYIIPLFLLLILFPWNTLAGTNVSPFVMVFE
                     KMNIPFAADIVNFVIVLALFSSINSGVYASSRILFFRLKDRKGSSRKLAVLNKHQVPQ
                     RAVLFCTSVLYLGVALSYFVGDKLFGYLAGSLSYTVLLIWIIISAASFVLALRKGTLW
                     DKGISFFALAVLGLIFLGILFTNSIGVTVLTGLLYLFIYFSYQKKNDAFVLTNE"
     gene            2404..3300
                     /locus_tag="U2K71_002997"
     CDS             2404..3300
                     /locus_tag="U2K71_002997"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002323722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide biosynthesis protein Alg14"
                     /protein_id="HEM7316986.1"
                     /translation="MNKNDVLLVAKNKSLYFLPSIRSLDVWSGKDLLQSEFIKSARQQ
                     LANYQLIVFLDYGFDMEMARRVRPFTNAKIVLFFWNHFKAEHEAMLKQAQDEPAIDEI
                     YHFDFLEAKELGLKHNSSFYSKHMNFSSSEPKTDLFFGATNNGRKERAESYKQEFIKR
                     DVTTNYFILPHRGNEQPGYLTYEEYLKKTGESRGILELLREGQQGVTLRTFESMYFQK
                     KLVTDNQAIAHYHFYRPENIFLLRERDLDELPEFLHTPFKPILSELLEFYDAENWAKR
                     FITNDSTIYEQYEYDLSSVENK"
     gene            complement(3450..4391)
                     /locus_tag="U2K71_002998"
     CDS             complement(3450..4391)
                     /locus_tag="U2K71_002998"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002376203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HEM7316987.1"
                     /translation="MKIAIAGAGAMGSRIGLMLHQSGNEVLLIDRWPAHIEAIRTNGL
                     IADFNGKEVVAKLPIYSPEEIIESNEHVDLIVALTKANQLDDMFCSIQSIITDNTYVL
                     CLLNGLGHEDVLEKYVPKKNILFGITMWTAGLAGPGKVTLLGDGEIELENLEPEGEAF
                     TKKVVEVFQEANLNPIYSHNVRYSIWRKACVNGTLNGLCTILDCNIAELGAQKAAESM
                     VRTIVSEFASIAAKEGIILDQEEVYQHIASTYDPDNIGLHYPSMYQDLIKNHRLTEID
                     YINGAIWRKGQKYDIATPYCAFLTQLVHAKEGILGAE"
     gene            4517..5638
                     /locus_tag="U2K71_002999"
     CDS             4517..5638
                     /locus_tag="U2K71_002999"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306095.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HEM7316988.1"
                     /translation="MDILAAIDSFKGSASSFELNQAVLNGWEDRKGRKINVPIADGGE
                     NTAEAIYYALRGTWRTIDGMDLLFRQRKISYLLTTWQGKNTAVIESAQALGLDLLDKP
                     TDQTIRLASSYALGELIKDALLQEVEQIIVTLGGSGCSDGGLGLLQSLGASLEGYTEG
                     NPLLSTKKLHFDRRNVLVSKVKLIIAADVTNPYTGQQGAAWQFGRQKGGIDTTLAFLD
                     KQAGKIAQQIKDFWEVDLDDIPGSGAAGGIGGALFLLGAKMVSGFDLVSQITGLEKKI
                     EQADVIITGEGRIDQQTIHGKVPYGVAVLAKKYRKPIIAICGSRDQELEGLSDFLPIV
                     LSIHLGPIGLEEAMEHRQTLEKTKILGQTLSRFFTILPK"
     gene            5896..7398
                     /gene="eat(A)"
                     /locus_tag="U2K71_003000"
     CDS             5896..7398
                     /gene="eat(A)"
                     /locus_tag="U2K71_003000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Eat(A)"
                     /protein_id="HEM7316989.1"
                     /translation="MSKIEIKNLTFGYDSQGTLLFEQANLNFDTQWKLGLIGRNGRGK
                     TTLLNILQNKLPYQGQVIHQQEFAYFPQQTKDKERLTYYVLNDITDFEIWEIERELQL
                     MQTDPEILWREFSTLSGGEKTKVLLALLFVDDTHFPLIDEPTNHLDISGRKQVAAYLK
                     KKKQGFIVVSHDRGFIDEVVDHVLAIEKSQLELYQGNFSIYEEQKKLRDEFEMAQNEK
                     LKKEVSRLKKTAAEKAEWSRSREGDKTKKQVGFIDTESRRVNKGAVGADAARTMKRSK
                     AIVNRMETQISEKEKLLKDIEYIDSLTMNSQASHHKRLLSVEDLQLGYENLLFEPIHF
                     TIEPHQRVAISGPNGAGKSSIIHYLLGAFNGKVIGEKYQPKHLSISYASQNYEDNRGT
                     LAEFAEKNQVDYQAFLNNLRKLGMERDVFHNKIEQMSMGQRKKVELAKSLSQPAELYT
                     WDEPLNYLDVFNQEQLEQLILNVKPAMLLVEHDQTFLDKVSTEIISLERI"
     gene            7426..7992
                     /locus_tag="U2K71_003001"
     CDS             7426..7992
                     /locus_tag="U2K71_003001"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HEM7316990.1"
                     /translation="MVKGNFANEGENHMLYVTRHGETTWNAQGLVCGRADVPLTEKGQ
                     MQAQKLAEKVVDLPVPITKIIHSPLQRARDTAQAVADRLSLPLTVDERLVEMDFGDYD
                     GLPSKDENFQKARLAFAVRFPNGESVLDVYARIVPLLKECIEDEENVYLLVCHNALIR
                     VINAYFHPMPNEGFFTFMVDNTELVSYE"
     gene            8203..9264
                     /locus_tag="U2K71_003002"
     CDS             8203..9264
                     /locus_tag="U2K71_003002"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF871 domain-containing protein"
                     /protein_id="HEM7316991.1"
                     /translation="MFGFSIFMNDSLSEEKKQYIRQMASNGFVGIFTSMHIPEDDVSA
                     YQKRLIELGSEAKKNKLELMVDISGEALERAGFSLEDLTPLKAIGVTGLRMDYHISNQ
                     QIADWSHQLKISLNASTITPKDIDELKEAEADFSQIEAWHNYYPRPETGLDKEWYQKK
                     NQWLKIQGLLVQGFVPGDTELRGPLYQGLPTLEEHRGIHPLAGALDLLASNTDIVYIG
                     DAGLSENVQEQFASFQKEQTVLLHTEPVDEEFYEYILGKHTNRQDDARDVIRSVDARF
                     REIPPIPARNTATRMKGSITLDNEKYLRYMGEIQLTKYDLPADEKVNVVAKVIKEELP
                     LINQIKAGMNYQFIRKEGR"
     gene            9267..10163
                     /gene="murQ"
                     /locus_tag="U2K71_003003"
     CDS             9267..10163
                     /gene="murQ"
                     /locus_tag="U2K71_003003"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002368658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HEM7316992.1"
                     /translation="MSKINLENLTTERRNQETFGLDEMSISQAVQLMNKEDQKVAAAV
                     EKELTSIEPVIANTIESFKKGGRLIYMGAGTSGRLGVLDAAECVPTFGVEPEMVIGLI
                     AGGEEAMTVAVEGAEDSYELGQEDLIKLNLTKNDIVIGIAASGRTPYVIGGLDYAKSI
                     GAVTGTISCNKQAEISKHADFPIEVDAGPEFLTGSTRLKSGTAQKLILNMISTISMIG
                     IGKVFNNLMVDVKPTNEKLIERSKRIIMQATEADYETAEKYFEEADQNVKLAIVMILT
                     NSDKEEAQAKLAEANGFVKGTL"
     gene            10177..12159
                     /locus_tag="U2K71_003004"
     CDS             10177..12159
                     /locus_tag="U2K71_003004"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002334912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS glucose transporter subunit IIA"
                     /protein_id="HEM7316993.1"
                     /translation="MAKNELTLEELAKQIYKGAGGMGNVESIVHCMTRVRMKIINDDL
                     VNVSELKAIPGVLGVVEDEQLQVIIGPGKVNKVAQQMVDLAGVKLGEKLSSVSTATAN
                     SSASGKDRVNERAQEMKAAQKSKQKPSKIKSVLKDISNIFVPMIPAFVGTGIVAGIAA
                     VLANLVTAGTLDAATWQQYIDVMNILKNGMFSYLVIYTGINAAQVFGATPTLGGVIGA
                     VVMLTGMNPEAPLKNLFTGEALAAGQGGILGVIFAVWLLSIVEKKLHKVVPDAVDIIV
                     TPTLSLIAIGLIEIFFIMPLAGFISDGMVGGINWVLGVGGAFAGFVLGTLFLPMVMFG
                     LHQILTPIHVQMIDETGRTLLLPILAMAGAGQVGAALALWIRCKKDKELTEMIKGALP
                     VGILGIGEPLIYGVTLPLGRPFITACIGGGIGGAVIGMIGNVGAIAIGPSGAALIPLI
                     SDGKWLGYILGLLAAYAGGFVATFFFGIPKDRLEKEELATETATVNEVPEKVVPQTSA
                     ESVTILSSVADGTVVSLDKASDPVFAQKMMGEGYFVEPENGHIYSPVTGVVSSVFPTK
                     HAIGITTPNGLEILLHMGINTVDLGGTPFDVKVTEGQQVTPDTLVAEADLEAIKAAGK
                     ETSMIVLVTNMDRVKNFVLEKTGKAKAKAPVMDVKA"
     gene            complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="U2K71_003005"
     CDS             complement(12224..12670)
                     /gene="nrdI"
                     /locus_tag="U2K71_003005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002299965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class Ib ribonucleoside-diphosphate reductase
                     assembly flavoprotein NrdI"
                     /protein_id="HEM7316994.1"
                     /translation="MNILYISISGNTRAFAKHLAEYAEKMHAEDPVNPEVTLKEIHEN
                     SDFTKETEPFFTFVPTYLDGGNGLDNGDTEILTETMREYLEHEDNHKLCLGVVGSGNK
                     NFNNQYCLTAKQYAQTFGFPFLADYELRGTPSDVTRIYQILAENNK"
     gene            12881..14305
                     /locus_tag="U2K71_003006"
     CDS             12881..14305
                     /locus_tag="U2K71_003006"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016853071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid permease"
                     /protein_id="HEM7316995.1"
                     /translation="MDTEQQKKGIGLIPLAALVIGSAIGGGVFGIMTDISGSAGGPAI
                     FSWVLVGTAMLMLSLSINNINKKRPDLKGGIFSYAEEGFGRFAGFISGWGYWLTCWLG
                     NVAFATLLMSALGYFFPVFEGGQNLASIVMSTVFLWGYAWLVNRGVENASFVNAIVTF
                     CKLVPLFLFIVVAISTFSIQQFIDNFSSDIILNANGQPIPFSSQVLSCIMVMLWVFVG
                     IEGASVVGSRAKKQSDVGKATILGIFGLIIIYVLVSMLPYGTMTMNQLQTIKAQPAMG
                     YVFEMMVGKWGAVVINGGLIISLVGVWLSWTILPIETMGNMAEDGLLPKAWGKLNKNG
                     APTYAIVLTTLCTNVFLLSLLFTDSAYNFAYTLGTAAIFFTWLFLGLYQMKLSYQRKE
                     WVQFLVGLLASAFQVWAMLVVAFNEVMLVLVLFLPGIYFYLKARKEQNEPVQHIHLDK
                     ILLIGVTMVGVIAMVLFATGMLHV"
     gene            complement(14486..15259)
                     /locus_tag="U2K71_003007"
     CDS             complement(14486..15259)
                     /locus_tag="U2K71_003007"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HEM7316996.1"
                     /translation="MTLLVKQELFKLIKKKSTAVLSVLLVVLLIGTALLAKKYTTIID
                     PVEMTAQLFSATSWIVFIMIAAASTIISMEAQYGTLKNLLYRKYSRGEILVSKWITLV
                     IYSVYLYLLAIIVTVLMKLILFPSISFTEKVSTGQTLIQSLFTYTLGSYIGLWLILSL
                     VLMIACFINSSGASISAGIVFYFASSIISGILIALIQKWEWIKWNPISMLNLQNQIGN
                     EEIMKQLTHLSTNQMLFGNLAYIVLFLALGYLVFKRKNV"
     gene            complement(15256..16161)
                     /locus_tag="U2K71_003008"
     CDS             complement(15256..16161)
                     /locus_tag="U2K71_003008"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002316458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HEM7316997.1"
                     /translation="MKSLSVQELSKNFGHKQALSDVSFSLSPGKIVGLVGPNGAGKTT
                     IMKAILGLIHYSDGTICIGENEISPSSHKGLEQVGALIEYPGIYPFLTGYDHLKLFSE
                     TNDVSAIDTIVNQLKMEKYIHKKAKSYSLGMKQKLGIALALLNHPSIVILDEPMNGLD
                     PQATRDVRKIITDLATQGTSFLISSHLLSELEKMVDELILINQGKIIKQCTMAELNQT
                     AQDFIVISTTNDLAARTILTEAGYTLEDPDKIKLLKENEELLGTVIQLLSAQNIHVTD
                     VQHLTNDLESSLLEILDSVKGEEEK"
     gene            complement(16315..16635)
                     /locus_tag="U2K71_003009"
     CDS             complement(16315..16635)
                     /locus_tag="U2K71_003009"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HEM7316998.1"
                     /translation="MAWMELIIAGILEVFWSTMMKWSDGFSKINYSFYTVIGMIASFY
                     FLSKAIKSLPMSLSYPIWTGIGAVGSVIIGVVLFHDKLNLSTWFFVGMLLISIIGIKI
                     TSGH"
     gene            17109..17795
                     /locus_tag="U2K71_003010"
     CDS             17109..17795
                     /locus_tag="U2K71_003010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010719881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF969 domain-containing protein"
                     /protein_id="HEM7316999.1"
                     /translation="MENYLSLLGVLIVILGFAFKLDSILIVMVALVVTALTGGLGIQG
                     MLELLGTSFVNNRGMAIFIIIMLATGTLERNGLKESAASLIKRFKKVSAGMIVDIYGI
                     FRMIFAAFNVSFGGVAGFVRPIILPMALGTIESKGLEVHPKHEEELKGMASAMENVCW
                     FFGQVLFIGGAGALLVQSTLKDLGYDVTLGQLALVEVPVAITALVVASVYFYLKEKKL
                     AKKYYPGKGQ"
     gene            17797..18741
                     /locus_tag="U2K71_003011"
     CDS             17797..18741
                     /locus_tag="U2K71_003011"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002306105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF979 domain-containing protein"
                     /protein_id="HEM7317000.1"
                     /translation="MDFFTNPDILLGDKLLEILYIVMGLVLIYTGVRNLTDKTNPHRY
                     GSAFFWTVLGVVIAGGRWLPAIVNGGLIFAMTIPAIAKKVSKGESRLPSKEYMEKMSD
                     KLGMKIFIPALSIGVFAILFALFTNLGALVGVGVGVFAAMLILMFFSRDNRPSTFLDD
                     AADMLGTVGPLSMLPMLLASLGAVFTSAGVGTVISNAVGTIVPEGNVNIGIIIYALGM
                     VIFTVIMGNAFAAITVMTVGIGAPFVFSHGANPALIGMVALTCGFCGTLLTPMAANFN
                     IVPVAMLEMKDKYGVIKNQLFIALFMLVFQIAYMILFK"
     gene            18940..19584
                     /gene="pcp"
                     /locus_tag="U2K71_003012"
     CDS             18940..19584
                     /gene="pcp"
                     /locus_tag="U2K71_003012"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010734749.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HEM7317001.1"
                     /translation="MKVLVTGFDPFGGDKVNPAYEAVKKMPDEISGAEIIKVEVPTVF
                     EKSSQVVKEAIQQHQPDVVICVGQAGGRSAVSFERVAINLAEARIPDNEGNQPFDTAL
                     EENGPAAYFTSLPIKAMTKNVQDHGLPAYISYTAGTFVCNDIMYRLLHLIETQFPAIR
                     GGFIHVPFSPDQVIDRPVGTPSMSLEDIASSLTYAVEAAVENKEDISGNAGTTH"
     gene            19663..20940
                     /locus_tag="U2K71_003013"
     CDS             19663..20940
                     /locus_tag="U2K71_003013"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002336015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7317002.1"
                     /translation="MEEWQSVFEEWFPKEISKSYPIKISKQYTSSQRWEIYAKLTKKQ
                     RELVDKHRRYLISSRFMEEHYLAATDWVFSDFKINPFFRTKRSQQKLYCECGRELKVQ
                     YIVKSPKTGKILKLGINHFADHLHVSPTVAASIHQGMTKVDLALDELLWLKQKNIDFP
                     EGLWQKYCFVLYQNRRMKQPYLPDIKLAQRLAEFRQVEMPIYIADYQALENEIKKISE
                     HINGQSKKRQIKKELFDDFAEELVKDVEEFLINYRAFLRKDWQSIVYEEVPVHPNAYF
                     ETFISVLRKTKRQRTPEVTAQMEYFAKNQRFIQPKIYLFIWKQYCRYGFTEGFFDSIP
                     RIVRNGFLKVLRKEREAIQSADKKDRTVSKEKWQLVVKDIQSGNVQETIDKWKGKHYR
                     FTEAQKQALEYYQKLEESLRFNDEARKYLKELL"
BASE COUNT         6778 a         3784 c         4362 g         6061 t
ORIGIN      
        1 ctgttcttat tacttgatgc agggcagctt tttcacaacc ccttattcac ggcgttccag
       61 aagtagcttc ttcttatttc ttgaagctga taacttctgg aacatcctct ttttttattt
      121 ggacggataa tggtcattcc atttctttga tcattttttt cattttgtcg atctcttctt
      181 cttgtgcctg aatgatttct tccgctaatt tttttacttc aggatctttt aaatcagctc
      241 gtttacttgc caggatagcg atagaatgat gaggaatcat ggccttcatc caagctgtgt
      301 cgttgacagt agtttggctt cgtaccataa acaaggaacc ggcaaaaaca agaagactag
      361 cggctaagat cataacattt atctttttgt tttcatacat tttccacatg aacaacagca
      421 tgatgactgc catgactgca cccatcatca acgccatgaa tactcgtgta cgactgaaga
      481 agatatgatc gagcgcatag acattcaagt acatcatacc aaacataact accgttgaaa
      541 caccgatcat caacaaaaac ttccaatact ttttcattta aaacaaactc ctttttttca
      601 ttcctttctt ctatcatgtc atgagcatgg agaacaatca attattttgc acagagggtt
      661 tttgaacacc gtttaccaag aggtcatgag actgtttatt cggaaaaagg agttgcaaca
      721 agacttttgt cacaactcct ttcttcaata gctccatcta ttccacataa ataaaataaa
      781 attttctatc gatttcattc attcgtcaat acgaacgcat cattcttttt ttgataactg
      841 aaataaatga acaggtaaag caggccagtc aatacagtta ccccaatgga gttcgtgaac
      901 aggataccaa ggaaaatcaa tcctaagaca gctaatgcaa aaaagctgat tcctttatcc
      961 cataatgttc cttttcttag tgccagtaca aaggatgccg cactgataat gatccagatc
     1021 aataatactg tatacgaaag ggaacctgcc agatagccaa ataatttgtc accgacaaaa
     1081 taagaaagag ctacaccaag ataaagaaca ctggtacaaa ataaaacagc acgttgcggt
     1141 acttgatgct tgtttaaaac agctagtttt ctagaggaac cttttcgatc cttcaaacgg
     1201 aagaacaaaa ttcttgaaga tgcatacaca cccgaattga tagatgagaa caaagcaagg
     1261 acaatcacaa aattgacgat atccgctgca aatgggatat tcattttttc aaataccatc
     1321 acaaaaggac tgacattcgt gcctgctaaa gtattccaag gaaaaagaat caaaagcaaa
     1381 aacaaaggaa tgatgtaaaa ggaaatgatt cttcccatga cacctttgat tgctttagga
     1441 atcgcacgtt taggatcatc tgcttcgctt accgtgatag caatcagttc agaaccgccg
     1501 taagaataga taactacaag caaagaactg atgattccct tcataccatg aggagtaaac
     1561 ccaccatggt cagtcaattg ctgaagggta gggaaaacgc ctgtgcccaa catttgctga
     1621 ccgacaagat aagtagcaaa aatgatcagt aagataatga cactgatttt agcaaaagcc
     1681 agccaatatt ctgtttctgc gaataaccgt accgaataaa gattgatgat cgtagttaaa
     1741 acagccaaca taaaaacaaa tacccaggct ggaatgtgcg ggaaccacaa ctgcaaaaag
     1801 gaagcagctg caacagcttc agcgatgata ttgataatcc acatagacca gtagacccaa
     1861 tcagtaaaat cagcagaatg agcacctaga taagggcgta tcaaaccaga taatccgtga
     1921 tgcgtgtcgc caccgcttaa aacgattttt cccagacctt tcatcacgat atataaaatc
     1981 agcccaccaa tcaaaaaagc aatcaataca gaagggccgg cgatcccaat cgcttcgcca
     2041 cttcctttaa atagacctgc accgattgct ccacctaaag ctaacatggt gatatgtctg
     2101 gagctaagtt gttttttcaa ttttttatct tccataaatg acctctttct tttaaattca
     2161 caatcaaaat ttatattaag aataaatagc aatatttaac aatgactaat gataacaaat
     2221 tttcagacaa ttacaactat tcagaaaact tttaggcgaa ttttaataga agaatgattt
     2281 atttcatcat attttcatac aaatgttaaa ttttcataca aatataggaa acgttttact
     2341 ggctttttac tatttaaaca ggctatcata ggaaaaagtg acagagaaag tgaggcggaa
     2401 aagatgaaca aaaatgacgt actgctagtt gcaaaaaata aatcacttta ttttttgcct
     2461 tccatccgct ctttagatgt ttggtccgga aaagatttac tacagtcgga attcattaaa
     2521 tcagctagac aacagttggc aaattatcag ctgatcgttt ttttagatta tggatttgat
     2581 atggagatgg ctcgacgcgt gcgcccattt accaatgcaa aaattgtgtt atttttttgg
     2641 aatcacttca aagcagaaca cgaggcgatg ctgaagcagg cacaagatga accggcaatc
     2701 gatgagattt atcattttga ttttttggaa gcaaaagaac ttgggctgaa acataatagt
     2761 tcattttatt cgaaacatat gaatttttct tctagcgaac caaagacgga tctatttttt
     2821 ggtgcaacga ataatgggag aaaagaacga gcagaaagct ataagcagga atttataaaa
     2881 agagatgtta cgaccaacta tttcattctt ccacatcgag ggaatgaaca acctggatat
     2941 cttacttatg aagagtacct gaaaaaaaca ggggaatcgc gcgggattct ggaactgttg
     3001 cgggagggtc agcaaggagt gactttgcgg acatttgaat ccatgtattt tcaaaagaaa
     3061 ctagtgacgg ataatcaagc gattgctcat tatcattttt atcgtcctga aaatattttt
     3121 ttgcttcgag aacgtgattt agatgaatta ccggaatttc tgcatacacc atttaagcca
     3181 atcctatcag agctattgga attttatgat gcagaaaatt gggcaaaacg atttatcact
     3241 aacgattcca ctatttatga gcaatacgaa tatgacctct cctctgttga aaacaaataa
     3301 aaaacctaca ttttgaagag attatgtact gaaacaaaca agcacaattc ttatggcatc
     3361 atgagagttg tgttttcttt ttgatctttt attgggctta aaaaaagaga accagctgct
     3421 gattcagctg attctcatca gagagccttt tattctgcgc caaggattcc ttcttttgca
     3481 tggactaatt gggtcaaaaa ggcacaatat ggtgtagcaa tatcatattt ttgtcctttt
     3541 cgccaaatgg caccattgat gtagtcgatt tcagtcaaac gatggttttt gatcaggtct
     3601 tggtacatag atgggtaatg caacccgatg ttgtcaggat cataagtaga agcaatatgt
     3661 tggtacactt cttcttgatc tagtatgatt ccttcttttg ctgcgataga agcaaattca
     3721 gatacgattg ttcgaaccat gctctctgcc gctttttgcg cgcctagttc agcaatatta
     3781 caatccaaaa tcgtgcataa accattcaat gttccattta cacaagcttt tcgccaaatg
     3841 gaataacgaa cgttatggct atagatcgga tttaaatttg cttcttgaaa gacttctaca
     3901 actttttttg taaatgcttc gccttctggt tccaagttct ctagttcgat ttctccatca
     3961 cctaacaatg tgacttttcc gggaccagct agaccagctg tccacatcgt gatcccaaat
     4021 aagatatttt tctttggcac atatttttcc agtacatctt catgacctaa tccatttagt
     4081 aaacacaaaa cataagtatt gtcagtaata atggattgga tcgaacaaaa catatcatct
     4141 aattgattgg cttttgttaa tgctacgatc aagtccacgt gttcgttgct ttcgatgatt
     4201 tcttctggtg aatagattgg cagttttgcg accacttctt taccgttaaa gtcagcaatc
     4261 aacccatttg tacgaatggc ttcaatgtga gcaggccaac gatcgatcaa gaggacctca
     4321 tttccgcttt gatgtagcat caatccgatt cggctgccca ttgctccggc cccagctatt
     4381 gcgattttca tgttttttct ctcctttatt caatgagttc ccttttagta tatagaaaaa
     4441 tggaagaaaa tagcattgtt ttgtttgaaa aaaatgaaaa aaagtgtctt acttaagaag
     4501 aaagtgaggg atacagatgg atattttggc ggcaattgat tcatttaaag gaagtgcttc
     4561 ttcatttgaa ctaaatcagg cagttctaaa tggctgggaa gatcgtaaag gaagaaaaat
     4621 caatgtgccg attgcagatg gcggagaaaa tactgcagaa gcaatctatt acgcgctcag
     4681 gggaacatgg cgtaccattg atgggatgga tctcttattc cgtcagcgaa aaatcagcta
     4741 tttattgaca acttggcaag gaaaaaacac cgcagtcatc gaatctgcac aagcattggg
     4801 gttagatcta cttgataaac caacggatca aactatccga cttgcttcca gttatgcctt
     4861 aggagaactt attaaagatg cactgcttca agaagtagaa caaattattg tcactttagg
     4921 aggaagcggg tgtagcgatg gtggtctagg attactccaa tccttgggtg cttctttaga
     4981 agggtacaca gaaggaaatc ctttattatc tactaaaaaa cttcattttg atcgaagaaa
     5041 cgttcttgtt tctaaagtaa aattgattat cgctgcagat gttaccaatc catatacagg
     5101 tcaacaagga gctgcttggc aatttggaag acaaaaagga ggaatagata cgacgctagc
     5161 tttcttagat aaacaagcag gaaagattgc tcagcaaata aaagatttct gggaggtgga
     5221 tttagatgat attccaggaa gtggagcagc aggcggtatt ggtggagccc tttttttgtt
     5281 gggagcgaag atggtttctg gtttcgattt agtcagccaa atcactggat tggaaaaaaa
     5341 gatcgaacaa gcagatgtca tcattacagg agaagggcgg atcgatcagc aaacgatcca
     5401 cggaaaagtt ccatacggtg tagcagtgct tgcaaaaaaa tatcgaaaac caataatcgc
     5461 tatttgcgga agtagagacc aagagctaga aggactttct gattttctcc caattgtctt
     5521 aagtattcat ttgggtccaa taggtttaga agaagcaatg gaacacaggc aaacattaga
     5581 aaaaacaaaa atcttaggac agacacttag cagatttttc accatcctcc cgaagtagtt
     5641 gacaaaatca ttttctttat gctatcgtaa tttcaaatat aggacaaagt gagggatccg
     5701 tgcattgaaa aattaagtcg attgatgaaa gctgcattca gactgtacct tgtggagaca
     5761 aggaagcagt gtgcagattt tcgggattgg cttggttttt tcgatgtatg ggtctttttg
     5821 tgtgtatcca acccttgcat cctctttagc cagttctgaa aataggactg gtcttttttt
     5881 acgaggtgat ggaaaatgtc taaaatcgaa ataaaaaatc tgacattcgg ctacgacagc
     5941 caaggcacat tattatttga acaagcaaat ctaaattttg acacacaatg gaaactagga
     6001 cttatcggac gaaacggtcg aggaaagaca actttactga atattctaca aaacaaacta
     6061 ccttatcaag ggcaagtaat ccatcagcaa gaatttgcct atttcccgca acagacaaaa
     6121 gataaagaac gtttaaccta ttacgtgtta aatgatatta cggattttga gatatgggaa
     6181 atcgaaagag agctccaatt gatgcaaaca gatcctgaaa tcttatggag agaattcagc
     6241 acactatcgg ggggagagaa gacaaaagtc ctactggcac ttttatttgt ggatgacact
     6301 catttcccgt taatcgatga accaacgaat catttggata tctctggtag aaaacaagta
     6361 gcggcttatt tgaaaaagaa aaaacaaggc ttcatcgtgg tcagccatga ccggggattt
     6421 atcgatgaag tagtggacca tgttttagca atcgaaaaaa gtcaactgga actttatcaa
     6481 gggaatttct ctatctatga agaacagaaa aaacttcgtg atgaatttga aatggctcaa
     6541 aatgaaaaat tgaaaaaaga agtcagtagg ctaaagaaaa cagcagctga aaaagccgaa
     6601 tggtctcgtt cccgagaagg agataaaaca aagaaacaag tcggattcat cgatactgaa
     6661 tctagacgag tgaataaagg agcagtgggt gctgatgctg cacggacgat gaaacgatcc
     6721 aaagcaatcg tgaatcggat ggagacccag atcagcgaga aagaaaaact attaaaagat
     6781 atcgaatata tcgattcttt gacgatgaat agccaagcgt ctcaccataa gcgactttta
     6841 agcgtagaag atcttcaatt agggtatgaa aatctgttat tcgagccaat tcattttaca
     6901 atcgagcctc atcagcgggt ggcgatttca ggtcctaacg gtgcaggaaa gtcatccatt
     6961 atccattatc ttctgggggc attcaacggc aaggttatag gagaaaaata ccagccaaaa
     7021 catctgagca ttagttatgc aagccaaaat tatgaagaca atcgaggaac gttggcggaa
     7081 tttgcagaga aaaaccaagt agactaccaa gcatttttga acaacctccg aaagcttggg
     7141 atggaaagag atgtttttca taacaagatc gagcagatga gtatgggcca acggaaaaaa
     7201 gtggaattgg ctaaatcttt atcacagcca gctgaactat atacatggga tgaaccattg
     7261 aattatttgg atgtcttcaa tcaagaacaa ttagaacaac tgatcttgaa cgtgaaacct
     7321 gccatgttac tagtggaaca tgatcaaacc ttcctggata aagtatctac tgagattatt
     7381 tctcttgaga gaatctaacc cttgtaatct caagaacaac aaggtatggt taaggggaac
     7441 tttgccaacg aaggagagaa tcacatgctt tatgtaacga gacacggaga aacaacttgg
     7501 aacgcacaag gattagtttg cggacgggca gatgtgccgc tgactgaaaa aggacagatg
     7561 caggcacaga aactagcaga aaaggtagta gatttgcctg ttccaatcac gaaaatcatc
     7621 cattcccctc ttcaacgagc aagagataca gcacaagcag ttgctgatcg tctcagcttg
     7681 ccgctgaccg ttgatgaacg attggtagaa atggattttg gggattatga tggactgcca
     7741 agcaaagatg agaactttca aaaagcacgt ttagcgttcg ctgtacgatt cccaaatggg
     7801 gagtcagtct tagatgtgta tgcacggatc gttcctttat tgaaagaatg catcgaagac
     7861 gaagaaaatg tctatctgtt agtttgccat aatgccttga taagagtgat caatgcttat
     7921 tttcatccga tgcctaatga ggggttcttt acattcatgg tagataatac agaattggtt
     7981 tcttatgagt aattatgttt ttgtgttatt taaataaaat atttcaaggc actgcttttg
     8041 cttagcagtg ccttttttag gttatgacaa ttcttttgat aatataaact caacctttag
     8101 aaatgaaatt taattatttt aataaaagta ttgaaatttt gtttctttag atatatactc
     8161 tagtcataga aatgaagacg gatacagaaa aagagggata atatgtttgg cttttcaata
     8221 tttatgaacg attctttgtc agaagagaag aaacagtata tccgccaaat ggcatccaat
     8281 ggctttgtcg gtattttcac ttccatgcat ataccagaag atgatgtttc ggcatatcaa
     8341 aagcgattga tcgaactagg aagcgaagcg aaaaagaata aactagaatt gatggtcgat
     8401 atttcaggag aggcattgga acgagcgggt ttttcattag aagatctaac gcctttgaaa
     8461 gcaattggtg taacaggttt aagaatggat tatcatattt ccaatcagca gattgccgac
     8521 tggtcacatc aattgaaaat cagtttgaat gctagcacga tcacgcctaa agatatcgat
     8581 gaattgaaag aagcagaagc tgatttctca cagattgaag cttggcataa ttattatcca
     8641 cgtcccgaaa cagggctgga caaagaatgg tatcagaaaa aaaatcaatg gctcaaaatt
     8701 caaggtctcc ttgtccaagg ttttgtacca ggagatacag agttgagagg cccactatat
     8761 caaggattgc caactttgga agaacatcga gggatccatc ctcttgcagg cgctctagat
     8821 ctattagcaa gtaacacgga tattgtttac atcggagatg caggtctgtc agaaaacgtt
     8881 caggagcaat ttgcttcttt ccaaaaagaa cagacagtct tgctccatac ggaacctgta
     8941 gatgaagaat tttacgaata tatattaggg aagcatacga atcggcaaga cgatgcaaga
     9001 gatgtcatac gaagcgtaga tgcacgtttt cgagagattc cgcctatccc agcaagaaat
     9061 acagctacgc gtatgaaagg gtctattact ttagataacg aaaagtattt gcgctatatg
     9121 ggtgagatcc aattaaccaa atatgatttg ccagcggatg aaaaagtgaa tgtagtagct
     9181 aaagttatca aagaagagct accattgatc aaccaaatca aagctggtat gaactatcaa
     9241 tttataagaa aagaaggaag ataaaaatga gcaaaatcaa tttagagaac ctaacaacgg
     9301 aacgcagaaa ccaagaaact tttggattag atgagatgag tatttctcaa gccgttcaac
     9361 taatgaacaa agaagatcag aaagtggccg ctgcggttga aaaagaactg acaagtatcg
     9421 aaccagtgat tgctaacacg attgagtcct ttaaaaaagg tggacgtctg atttatatgg
     9481 gagccggaac aagtggaaga ctaggtgtgt tagatgctgc tgaatgcgta ccgacattcg
     9541 gtgtagaacc ggaaatggtc ataggtctaa ttgctggtgg agaagaagca atgactgtag
     9601 ctgtcgaagg ggccgaagat tcttatgagc tagggcaaga agatctaatc aagctgaatt
     9661 tgacaaaaaa tgatatagtt attggtatcg cagctagcgg tcgtacacca tatgtgatcg
     9721 gtggattaga ttacgcaaaa tcgatcggtg ctgtcacagg aacgatttct tgcaataaac
     9781 aagccgaaat cagtaaacac gcggatttcc caatcgaagt cgatgcaggt ccagaatttt
     9841 tgacaggctc tactcgattg aagtcaggaa cagcacaaaa acttatcttg aatatgattt
     9901 ctactatcag tatgattggt ataggtaaag tattcaataa cttgatggtc gatgtcaaac
     9961 caacaaatga aaaactaatc gaacgctcaa aacgaatcat catgcaagcg acagaagctg
    10021 attacgaaac agctgaaaag tactttgaag aagcagatca aaatgttaag ctggctatcg
    10081 tcatgatttt gacaaatagt gataaagagg aagctcaagc aaaattagct gaagcaaacg
    10141 gatttgtaaa agggacctta tgagggggaa aagaccatgg caaaaaacga actgactttg
    10201 gaagaactgg caaaacaaat ttataaaggg gcaggcggca tgggcaatgt agaaagcatt
    10261 gtccactgca tgactcgtgt gcgtatgaag atcatcaatg atgatcttgt aaatgtctct
    10321 gaattaaaag cgattccagg agtgcttggt gtagtagaag atgaacagct tcaagtcatc
    10381 atcggacctg ggaaagtgaa caaagtagca cagcaaatgg ttgatttggc aggcgtgaaa
    10441 ttaggagaaa aactttcttc tgtaagtaca gctacagcaa attcatcagc atctggaaaa
    10501 gaccgtgtaa acgaacgggc acaagaaatg aaagcggcac aaaaatcgaa acaaaaacca
    10561 tctaaaatca aatcggtatt aaaagacatt tctaatatct ttgtgccaat gattcctgca
    10621 tttgtcggta ctgggatcgt ggcagggatt gccgcagttt tagcaaactt agtcacagcc
    10681 ggaacacttg atgcagcaac atggcaacaa tatatcgatg tgatgaacat cttgaaaaac
    10741 gggatgttta gctacctagt tatctataca ggtatcaatg ctgcacaagt ttttggtgca
    10801 acaccaactc ttggtggagt tatcggggcc gttgtgatgc tgacagggat gaatccggaa
    10861 gcaccactga aaaacttatt tactggtgaa gctcttgcag ctggacaagg tgggatccta
    10921 ggtgtcatct tcgctgtttg gctattgtca atcgttgaga aaaaactgca taaagtagta
    10981 cctgatgcgg tagatatcat cgttacacca actctttcat tgattgccat cggtctgatt
    11041 gaaattttct tcatcatgcc tttagcaggt ttcatttctg atggcatggt cggtgggatc
    11101 aactgggtac ttggcgttgg tggagcattt gccggcttcg ttctaggcac attattcttg
    11161 ccgatggtta tgttcggact gcatcaaatt ttgactccaa tccatgtgca aatgattgat
    11221 gaaactggtc gtacgctatt gcttccaatt cttgcgatgg ctggtgcagg acaagtgggt
    11281 gcagcattgg cattatggat tcgctgcaaa aaagataaag aattaacaga aatgatcaaa
    11341 ggagctttac cagtcgggat cctaggaatc ggcgaaccat tgatttacgg agtaacatta
    11401 cctctaggac gcccatttat tactgcttgt atcggtggag gtatcggcgg ggcagtgatc
    11461 ggaatgatcg gaaatgtcgg ggctatcgct attggacctt caggagccgc tttgatccca
    11521 ttgatctctg acggtaaatg gttaggctac atccttggat tattggcagc ttatgctggt
    11581 ggcttcgttg ctacattctt cttcggtatt cctaaagacc gtttagaaaa agaagaatta
    11641 gcgactgaaa cagccacagt aaatgaagtg cctgaaaaag tagtacctca aacttcagct
    11701 gaaagtgtga ctatcctctc atcagttgct gacggaacag ttgtttcttt agataaagca
    11761 agtgatccag tttttgcaca aaagatgatg ggtgaaggct actttgtaga accagaaaac
    11821 ggacatatct attctccagt tactggggta gtcagctctg tcttcccaac gaaacacgct
    11881 attgggatca caacgccaaa tggtttagaa atcttgcttc atatgggaat caacacagtt
    11941 gacttaggcg gtacgccatt tgatgtgaaa gtaacagaag ggcagcaagt gacacctgat
    12001 acgcttgtag cagaagctga cttagaagcg atcaaagcag caggcaaaga aacatccatg
    12061 atcgtcttgg taaccaatat ggatcgtgta aaaaactttg tcttggaaaa aacaggtaaa
    12121 gcaaaagcaa aagcacctgt gatggatgtc aaagcttaat atgatagaaa caatagaaac
    12181 gaaagaagaa actgaaatct atttttcagc ttcttctttt ttattattta ttgttttctg
    12241 ctaaaatctg ataaattcgt gtcacatcag atggtgttcc ccgtaattca taatctgcca
    12301 agaaaggaaa gccaaatgtt tgtgcatatt gtttggctgt tagacaatac tgattattga
    12361 agtttttatt tccgctaccg actacaccaa gacaaagttt atgattgtct tcatgttcca
    12421 gatattcacg catcgtttcg gtcaagattt ctgtatcacc gttatctaaa ccatttccgc
    12481 cgtctaaata agtaggaacg aaagtaaaaa atggttctgt ttctttcgta aaatcactat
    12541 tttcatggat ttcttttaaa gtaacttcag gattgacagg atcttctgca tgcatttttt
    12601 cagcatattc tgctagatgt ttggcaaagg cacgcgtatt tcctgaaatc gatatatata
    12661 aaatgttcat ggatcatcct ccaattcttt ccttttagta tagcatcttt tttaagtttt
    12721 ttggtggtgt aatttggaaa gggactaggc gctttgcttg tataatgaga aaaaagccgg
    12781 tagaattata tttctattaa agtttttatt aattaagtaa aaataaaaaa ggtttgttat
    12841 actttttgat aaaagcggtt ttattttgga ggatgcttac atggatacag agcaacagaa
    12901 aaaaggaatt ggtttaattc cacttgctgc cttagtaatt ggttcagcga ttggaggtgg
    12961 cgtatttggt attatgacgg acatctccgg atcggctggc ggcccagcta ttttcagttg
    13021 ggtactcgta ggaacagcaa tgttgatgct ctccttgtcg atcaataata tcaataaaaa
    13081 acgtccagat ttaaaaggcg ggatctttag ttacgcagaa gaaggttttg gccgtttcgc
    13141 tggttttatc agcggttggg gttactggct cacatgctgg ttaggcaacg tagcttttgc
    13201 aacattgttg atgagcgcat taggatattt cttcccagta tttgaaggtg ggcaaaatct
    13261 ggcatcgatc gtgatgagta cagtcttttt atggggatat gcatggctag tgaatcgggg
    13321 agtcgaaaat gctagtttcg tcaatgctat cgtcacattc tgtaaattag tgccattatt
    13381 tttatttatt gtagtagcta tcagcacgtt ttcgatccaa caattcattg ataacttttc
    13441 tagcgacatt atattgaatg cgaatggaca gccgatcccg ttttccagtc aagtattgag
    13501 ttgcatcatg gtgatgcttt gggtatttgt tggtattgaa ggagcctctg tcgtaggttc
    13561 acgagcaaaa aaacaatcag atgttgggaa agcaacgatt ctagggatat tcggcttgat
    13621 tattatttac gtcttggttt ccatgcttcc atatgggaca atgacgatga atcagctgca
    13681 aacgatcaag gcgcagcctg ctatgggcta tgtgtttgaa atgatggttg gtaaatgggg
    13741 agcagtcgtg atcaacggcg gtttgatcat ttctttagtt ggtgtttggc tctcatggac
    13801 gatcttaccg attgaaacaa tgggaaacat ggctgaagac ggacttttgc ctaaagcatg
    13861 gggaaaactc aacaaaaatg gggcaccaac ttatgctatc gtattgacga cgttatgtac
    13921 gaatgtcttt ttactttcat tattattcac tgacagtgct tataactttg cttacacact
    13981 tggtacagca gctattttct tcacgtggct tttcttagga ttgtaccaaa tgaaactttc
    14041 ttatcagcgg aaagaatggg tccaattttt agtagggctt ttagcaagtg cctttcaagt
    14101 atgggctatg ctagtcgtag cctttaatga agtaatgctt gttcttgttc ttttcttgcc
    14161 aggcatttat ttttacctca aagcacgtaa ggagcaaaac gagcctgtac aacatatcca
    14221 cttagataag attttactta taggtgtgac gatggtcgga gtgattgcaa tggttttatt
    14281 tgccactggg atgcttcacg tatagaagta taaaagaagt ataaagagag gttgtgagcc
    14341 tgtcgtttat tcaaaaacat actaggaaag gagctgtgac acaaatgttg tcacagctcc
    14401 tttttcagaa taaacggtgt ttaagagaca gccaatcggc aataaggctg aacgtctctt
    14461 tcacagcctc ttttttgatg agtcatcata cattttttcg cttaaatacg agatatccta
    14521 atgctaagaa caaaacgatg taagctaaat taccgaatag catttgatta gtagaaagat
    14581 gagtcaattg cttcatgatc tcttcgtttc caatttggtt ttgcagattc aacatactga
    14641 tcggattcca tttgatccac tcccattttt ggatgagtgc aataaggata ccagatatga
    14701 ttgacgaagc aaaataaaag acgattcctg ctgagatgga tgcgccggaa ctattgataa
    14761 agcaagcaat catcaacact aaacttaaaa tcagccataa accgatataa ctacccaaag
    14821 tataagtgaa caagctttgg atcagtgttt gaccagtaga aaccttctcc gtgaaggaaa
    14881 tcgaagggaa aagaatcagt ttcatcaaca cagtgacaat aatagctaaa aggtataaat
    14941 aaacagaata aataactaat gtgatccatt tgcttactaa tatttctcct cgagaatact
    15001 ttcgataaag gagatttttc aatgtaccat attgtgcttc catcgaaatg atcgtgcttg
    15061 cagctgcaat catgataaag acaatccaag atgtggcact aaaaagctga gcagtcattt
    15121 caactggatc aatgatcgtc gtgtattttt tggctagaag tgctgttcct atgagcaaaa
    15181 caactaatag aacagataaa acagcagttg actttttctt gatcaattta aataattctt
    15241 gttttacaag caatgtcatt tttcttcttc cccctttact gaatccagaa tttccaatag
    15301 agaagattcc aaatcattcg tcagatgctg gacgtctgtc acatggatat tttgtgcaga
    15361 caatagctgg atcacggttc ctaataattc ttcgttttct tttaaaagct tgattttatc
    15421 aggatcttcc aaagtatatc cagcttctgt caagatcgtt cgagcagcta gatcatttgt
    15481 tgtagagata acgatgaaat cttgcgctgt ttggttcaat tcagccattg tacattgttt
    15541 gatgatcttt ccttgattga tcaggatcag ctcatcgacc attttttcaa gctcgctgag
    15601 taagtgactg gagatcaaga aagaggtgcc ctgagtagcc aagtcggtaa tgatttttcg
    15661 cacatctctt gttgcttgtg gatccaaacc gttcatcggc tcatctaaaa taacgataga
    15721 cggatggttc agtaaggcaa gagcaattcc caatttttgt ttcattccca acgagtagga
    15781 tttcgctttt ttgtgaatgt acttttccat cttgagctga ttcacgatag tgtcgatagc
    15841 agatacatcg ttggtttcag agaataactt cagatgatca tagcctgtta agaatggata
    15901 gatacccggg tattcgatca atgctcctac ttgttccaac cctttatgac tagagggaga
    15961 aatctcattt tcaccaatac agattgttcc atctgagtaa tggatcagtc ctaaaatagc
    16021 tttcatgatc gttgtttttc cagcgccatt aggacctact aatccaacga ttttgccagg
    16081 ggataagcta aaactgacat cgctgagggc ttgtttgtga ccaaagttct tggataattc
    16141 ttggaccgaa agacttttca aaaatattca ctccttttct atagatagta caagcataca
    16201 cctgatgagt ggagaagtcg ctaggtcatt ggacttattt cttatttttt cataaaaaaa
    16261 ttgaatatta aataaaaagg cttatttttt ctgatcagta cttattccaa gtgtctagtg
    16321 gccgctagta atcttgatgc caatgatact gatgagtagc atccccacaa agaaccaagt
    16381 ggaaagattc agcttatcat gaaacaagac cacaccaatt atgaccgaac caactgcgcc
    16441 aatccctgtc cagataggat aagagagact catcggcaaa cttttgattg cctttgacaa
    16501 aaagtaaaaa ctagcaatca tgccaatcac tgtgtaaaaa gaataattga ttttagaaaa
    16561 accatcactc catttcatca tcgttgacca gaatacttcc aaaattcctg ctatgattaa
    16621 ttccatccat gccatactta cattcctcct taactataaa aaaatacaca aaaagaggaa
    16681 aatcatccct tcaaagacct aatggaatga tcatcctctt tatcctaccc ggtggcagat
    16741 tgtatatctt tttctgtagt atagcacacc tatccagata tcggcaatct catacttttt
    16801 agtaaagtgt accaagcact gacggatata tcgttagcgg aaaagaagat gaatgctttg
    16861 caatttcata gaaagaagtt gttatttatt aatcatttaa aaaaaacagt aactagacct
    16921 tatttctata tattatcggg caataaatta aaaaaagctt taccttacaa taagtaagat
    16981 aatttttgtg ataaaaaaat aacgaaaaat atttaaaaat agtgtctatt tcgtgagaaa
    17041 acaactatat tttttatgtg ttttgcttta taattaaaaa aatattgtca ttttatggag
    17101 gaaaatatat ggaaaattat cttagccttt taggcgtctt gattgttatt ctcgggtttg
    17161 catttaaatt agactcgata ttgatcgtaa tggttgcttt agttgttaca gctttgactg
    17221 gcggtcttgg cattcaagga atgttagaac ttttaggtac aagttttgta aacaatcgcg
    17281 ggatggcgat ttttatcatc attatgttgg caacaggaac gcttgaaaga aatggtctga
    17341 aagaatcagc ggcttccttg atcaagcgat tcaagaaagt atctgctggt atgattgtag
    17401 atatttatgg gatctttcga atgatctttg cggcttttaa cgtgagtttt ggtggagttg
    17461 ctggatttgt tcgtccaatc attttaccga tggcattggg gaccatcgaa tcaaaagggt
    17521 tagaagtcca tcctaagcat gaagaagaac taaaaggtat ggcttcagct atggaaaatg
    17581 tctgctggtt ctttggccaa gtactattca tcggaggagc aggggcgttg ctagtccaat
    17641 caacactgaa agatctagga tatgacgtaa cattgggaca gcttgcttta gtggaagttc
    17701 cagtggctat cacggcttta gttgtggcaa gtgtctactt ctatcttaaa gaaaagaaat
    17761 tagcgaagaa atattatcca ggaaaggggc aataagatgg acttttttac aaatcctgat
    17821 attttattag gcgataaatt actggagatc ctttatatcg tgatgggact cgtattgatt
    17881 tacactggag taagaaattt aacagataaa accaatcctc atcgttatgg gtcagccttt
    17941 ttctggacgg tcttaggggt agttattgca ggaggaagat ggcttccggc aatcgtgaac
    18001 ggtggcttga tctttgcgat gaccatacca gcaattgcta agaaagtcag caaaggagaa
    18061 agccggcttc catcaaaaga atatatggaa aaaatgtcag acaaactagg aatgaaaatt
    18121 tttattccag ctttgagtat cggtgtcttt gcgatcttgt ttgctctatt taccaattta
    18181 ggtgctttag tcggcgtggg cgtcggtgtt tttgcagcca tgctaatctt gatgttcttt
    18241 tctcgagata atcgaccaag tacattttta gatgatgcag cagatatgct gggcacagta
    18301 gggccgttaa gcatgttgcc aatgctttta gcttctctag gtgcggtatt tacaagcgct
    18361 ggagttggga cagtgatttc caatgcagta ggtacgatcg ttccagaagg aaatgtgaac
    18421 atcggcatca tcatttacgc tcttggcatg gtgatattta cggtaatcat ggggaatgca
    18481 tttgcggcca tcaccgtgat gacggttggg atcggtgcac cattcgtatt tagtcatgga
    18541 gcgaatccag cgttaatagg aatggttgca ttgacttgcg gtttttgtgg gaccctgctg
    18601 acaccgatgg ctgctaactt caatatcgtg cctgtagcga tgctggagat gaaagacaaa
    18661 tacggagtga tcaaaaacca gttgtttatt gcgttattta tgctagtttt ccaaattgct
    18721 tatatgattc tatttaaata atagaagaga ggggttgtga cagaataatt gtcgcaacct
    18781 cttctaacat tgtagtggaa cactattttt ttataagaat atattaagtt tgatctgtta
    18841 tgttatttaa actattgttt cctttattta cgctgttcaa ggaaaatggt atgataaaag
    18901 ataaccatat gttaaatcaa tcggacggag gaacaacaga tgaaagtact agtaacaggt
    18961 tttgatccat ttggtgggga caaagtgaat ccagcctatg aagcagtaaa aaaaatgcca
    19021 gatgaaatca gcggtgctga aatcatcaaa gtggaagtac cgaccgtatt cgaaaaaagc
    19081 agtcaagtag tcaaagaagc catccaacag caccaaccag atgtcgtgat ctgtgtcgga
    19141 caagcaggtg ggcggtcagc agtctcgttc gaacgtgtcg caatcaatct agcagaagca
    19201 agaatccctg ataatgaagg aaatcagcca ttcgatacgg ctttagaaga aaacggtcca
    19261 gccgcttact ttacctcatt gccaatcaaa gcaatgacaa aaaatgtaca agatcatggt
    19321 ctaccagcgt acatctctta tacagcagga acatttgtct gcaatgatat catgtatcgt
    19381 ttgcttcatt tgatcgaaac acaattcccg gcaattcgag gtgggttcat ccacgtgcca
    19441 ttctcacccg atcaagtgat cgatcgtcca gtgggtacgc cttctatgtc tttagaagac
    19501 attgcttctt ctttgactta tgcagttgaa gcagcagtag aaaataaaga agatatttcc
    19561 ggaaatgccg gaacaactca ctaatttagc aagaaacact ggctggttgc taacgttatg
    19621 ttagtagcca gctttttgta attagaaagg aaagaaacag ccgtggaaga atggcaatcc
    19681 gtctttgaag aatggtttcc aaaagaaatc agcaaaagct atcccatcaa aatttctaaa
    19741 caatacacaa gcagtcaacg ttgggagatc tatgcaaaat taacgaaaaa acagcgggaa
    19801 ctagtcgata agcaccgccg atatctaatc agctctcgtt ttatggaaga acattattta
    19861 gctgcaacag attgggtgtt ttctgacttc aagatcaatc cgttttttcg aacaaaacga
    19921 agccagcaaa aattgtattg tgagtgtggt agagaattaa aagtgcaata catcgttaaa
    19981 tcgccaaaaa ccggaaagat cctaaaacta ggcatcaacc actttgccga tcacctgcat
    20041 gtctctccga cagtggcagc ttcgatccat cagggaatga caaaagttga tctggcctta
    20101 gacgaattat tatggcttaa gcagaagaat atcgattttc ctgaaggact atggcaaaaa
    20161 tactgttttg tcttgtacca aaatcgtcgg atgaaacagc catatttacc ggatataaaa
    20221 ttagctcagc ggctcgcaga atttcgccaa gtagaaatgc cgatttacat agcggattat
    20281 caagcattgg aaaatgaaat caagaaaata agtgaacaca tcaatgggca gtcaaaaaag
    20341 cgacagataa aaaaagaact atttgacgac tttgcagaag aattagtaaa ggatgtagaa
    20401 gaattcttga tcaactatcg cgcattttta cgtaaagatt ggcaatccat cgtatatgaa
    20461 gaagtgccag tacatcctaa tgcttatttt gaaacattta tttccgtgtt gagaaagaca
    20521 aaacgtcaac gaacaccaga agtcactgct cagatggaat attttgcgaa aaaccaacga
    20581 ttcatccaac caaaaatcta tctatttatt tggaagcaat attgtcgcta tggttttaca
    20641 gaagggttct tcgacagcat tcctcggata gtcagaaatg gttttttaaa agtattgcgt
    20701 aaagaacgag aagctataca atctgctgat aagaaagacc gtacagtctc aaaagaaaaa
    20761 tggcaactag tagtgaaaga tatccaatct ggaaatgtgc aagagacaat cgacaaatgg
    20821 aaaggaaaac attatcgttt tactgaagct cagaaacaag cgttggaata ttatcagaag
    20881 ttagaagaat cactgcgttt caatgatgag gcaagaaagt atctgaagga attactataa
    20941 aaaatagagg ctgggacaga agcgtttaac tccaagaaat aagaa
//
LOCUS       DASHHB010000208        13218 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.069, whole genome shotgun sequence.
ACCESSION   DASHHB010000208 DASHHB010000000
VERSION     DASHHB010000208.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 13218)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13218)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13218
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.069"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(204..2162)
                     /locus_tag="U2K71_003014"
     CDS             complement(204..2162)
                     /locus_tag="U2K71_003014"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002335294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PTS transporter subunit EIIC"
                     /protein_id="HEM7317003.1"
                     /translation="MKAYLQRMGRSLQLPVAVLPAAALLVGIGNWWASYSNDIVAHFL
                     QAGGNAVLGQLPLLFAVGLALGMSVDKSGAAALAGLVAFEVPVNVLKTDSVATLLNIK
                     PESVDPSFAQISNVFIGIIAGLIAAALYNRFHEAKLPMALSFFSGKRLVPILAAFVML
                     LISAVLLLVWPPVYSALVSFAKFILSLGWGGAGLYGFFNRLLIPTGLHQAMNSVFWFD
                     VAGINDIGKFLASEGTKGVTGMYQAGFFPIMMFGLPAGAYAIYRNARPENKAKTASLM
                     LAAAFASFFTGVTEPLEFSFMFVAWPLYVLHAIFTGISLAVSAFFHWTAGFAFSAGFV
                     DYFLSLKNPIANQPLMLIVQGLVTAVIYYFGFNFAIKKFHLMTPGREEADLSDDDTAT
                     TNSDNKYAAQASKIYAALGGADNVTSIDNCTTRLRLQVKDTGLIDQNKIKATGVPGMK
                     IIDGKNAQVIVGTEVQFVADEMAKLHGGAATRPAQTNTVVKTETFAGGTIEQDIYAIA
                     NGKLIPITEVSDDVFAEKMMGDGYAVLPENGEIFSPIAGTITNIFPTKHAVGIQTDAG
                     IEVLLHMGINTVDLKGEPFTLYVKEGQKVARGQLIALVDLAAIQSAGKNTDMVVVFTN
                     GDKVQSLEIEPARDVKANDKIGSVSNKA"
     gene            complement(2159..3031)
                     /locus_tag="U2K71_003015"
     CDS             complement(2159..3031)
                     /locus_tag="U2K71_003015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005874851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRD domain-containing protein"
                     /protein_id="HEM7317004.1"
                     /translation="MKIKKIFNQNAVLVDDGGQEKVAIGKGIGFDRKRNDLIFDRDVE
                     RIFVMEPEGQIKLQNLLNQIDERFLFAAETIIDHAETVLMEKLNEHVLIALTDHIAFS
                     AENINNGIVIRNKLLREIEVLYSDEFSIAQWAVEYLTKELGIPYTYDEAGYIAIHIHS
                     GRSGRNNNHRSIREVTIVSDIIHLVESELETDIHSEPFSLNYSRLVNHLRLLLQRTHA
                     QQYAVLDTEIVEMVKRKYPESYKISKKIRVLLTKEYQLAITKEELGYLAIHIERLRSA
                     IVQTNVNNHEEEKN"
     gene            complement(3373..4245)
                     /locus_tag="U2K71_003016"
     CDS             complement(3373..4245)
                     /locus_tag="U2K71_003016"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HEM7317005.1"
                     /translation="MDIRILTYFLAVAREKNISRAAKSLHITQPTLSKQLKDLEEELG
                     VTLFTRGSREIQLTDSGRYLYSKGKEILSLVEKTANNLTNDAIISGEIYIGGGETKAI
                     SFISDSLHSLIKAHPDIQFHLYSGNADDIIDKLDSGLLDFGLVIEPTNKQKYQSFRLP
                     AQDRWGLLIHRNHPLAKQTVIHPQELKTVPLIISRQTSVDSQLAEWLGFSIDQLSIVG
                     TYNLLYNASLMVDNGTFGALALDGIINTYGTEKVFIPLDPPLQASLNLIWKKDQPLSP
                     AARKFLDVLTEKYS"
     gene            4373..4942
                     /locus_tag="U2K71_003017"
     CDS             4373..4942
                     /locus_tag="U2K71_003017"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002337402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HEM7317006.1"
                     /translation="MTSLIEKQIVNKEIRVTDPLFEVIHQIQAENEEPLAALNTGYHE
                     PADIRKRLENIISDKVDDTVTVLLPFYTDFGKHISIGKNVFINRQVMFVDLGGICLED
                     SVLIGPRVNLITVNHLTDPKERRGLSVKPIHIKKNAWIGAGATILPGVTIGENAIVAA
                     NATVTKDVPDNTIVAGIPAKIVKPVERII"
     gene            5009..5746
                     /locus_tag="U2K71_003018"
     CDS             5009..5746
                     /locus_tag="U2K71_003018"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002325513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HEM7317007.1"
                     /translation="MNDLSEKVIVIMGASSGIGEATTKLLAEKGAKLVIAARREDRLK
                     AIKESLPEAELYIQTADVRDFAQVQAVIDLAMEKFGRIDVLYNNAGIMPTAPLVEGHR
                     DEWQNMLDINIMGVLNGISAVLPIMEKQKSGHIISTDSVAGHVVYPDSAVYCGTKFAV
                     RAIMEGLRQEQRQNNIKSTIISPGAVQTELYQTISDKKAALELHEAQKEWGLTSEDIA
                     SAVAFAIETPDRMSVSNMIIRPTTQEV"
     gene            5977..7245
                     /locus_tag="U2K71_003019"
     CDS             5977..7245
                     /locus_tag="U2K71_003019"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002344825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 73 protein"
                     /protein_id="HEM7317008.1"
                     /translation="MKAKNLLFSVILLGNLAVANTTVLAEESTETVATQTSSSSETGI
                     ENDTESTLQSATVSENTISTEEPTTETTEQTQTEDSTSAEAQNNPENAEEITNQLTDG
                     QTLTHTHQADLSEFENYTGYAFYARSSANSQQAFIDSIASTAQSLASANDLYASVMIA
                     QAIVESGWGNSTLASAPNYNLFGIKGSYNGQSVTMPTSEYVNGQWITVNAAFRKYPSY
                     KESLQDNVTVLKTTSFQPGVYYYSGAWKSNTNSYKDATAWLTGRYATAPNYGSTLNNV
                     IETYNLTQYDTAPTSTSPMYRLYNRHTGEHLYTLNAGEKNYLPTVGWEYEGIAWQAPN
                     SGQPVYRLYNPYSGDHHYTMAQSEINSLTKIGWRYEGLSFYSGGSKPIYRLFNPNEKT
                     GTHHYTLSAKERDWLTPMGWRYEGIGFYGY"
     gene            7732..8196
                     /locus_tag="U2K71_003020"
     CDS             7732..8196
                     /locus_tag="U2K71_003020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HEM7317009.1"
                     /translation="MSLEEISHLLYQIKLADQKIVQIFEENIGISLTRYEIMMRLKEE
                     NPLLQSSLQEKLQIDQGAVTRHLKVLEANGYVSRQRNPQNNREVYVALTEKAQKKIKH
                     CEMDHKDTGSVLSPAFSDVEMKQFLTLLDKLNTQLCNITTETKEVNNNGTKK"
     gene            8180..8809
                     /locus_tag="U2K71_003021"
     CDS             8180..8809
                     /locus_tag="U2K71_003021"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002287459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HEM7317010.1"
                     /translation="MEQKNDFSTIMTGRRSVRVYDETYKIPHEEMLEMIKEATTAPSS
                     VNMKPWRFVVAESEEAKEILRPLIRFNTRQNDTSSAMVMIFGDMQCYEYGEEIYNQAY
                     ESGKMPKEVRDQQLAAIIPYYKSFSREEMNDVVKVDSSLAAMQFMLVARDHGYETNPI
                     GGFEADQLAEAFGLDKDRYVPVIILSIGKAVEEGYESVRLAPEKITMFE"
     gene            9163..10641
                     /gene="msr(C)"
                     /locus_tag="U2K71_003022"
     CDS             9163..10641
                     /gene="msr(C)"
                     /locus_tag="U2K71_003022"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002314314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F type ribosomal protection protein Msr(C)"
                     /protein_id="HEM7317011.1"
                     /translation="MENLAVNITNLQVSFGNQLELSIDSLRVYQQDRIGIIGENGVGK
                     STLLKLIAGELFPDHGKIQTEITFNYLPQLTYLAEAKDLNLELASHFQLRLEETSERK
                     WSGGEERKIELIRLLSSYEQGMLLDEPTTHLDRKSIDRLIEELRYYYGTLVFVSHDRY
                     FLDELASKIWEVKDGEIREFSGNYSAYLTQKELEKKTQLREAESIMKEKKRLEKSIQE
                     KKKQAEKLEKVSSKKKKQQIRLDRLSSSKQKDSVQKAIQKNAKTLERRLQKIGETTKP
                     QQMKQIRFPVPKSLELHSRYPIMGQNVQLERSGRTLLVNGDFQFSLGKKIAIVGENGS
                     GKTTLLEHIRKQGEGILLSPKVSFQVYQQKGYQMTSEESIIRFVMRQTEFSESLVRSL
                     LNHLGFAQETLTKPLCTLSGGEATRLTIALLFTKPSNVLLLDEPTNFIDMATIEALEK
                     LMQIYPGTILFTSHDSYFVERTADEVYEIKGQKIKKVLTRNF"
     gene            10688..11134
                     /locus_tag="U2K71_003023"
     CDS             10688..11134
                     /locus_tag="U2K71_003023"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2798 domain-containing protein"
                     /protein_id="HEM7317012.1"
                     /translation="MPTNKKEGIIFTTMMCFLMVLGMSAYNLWLHQDLHLSSLLIGLV
                     PGFVVAFILDVFIIGVLAKKIAFKLPVNPESKVQMILTISCLMVLGMVTCMSLFGVIM
                     EQGIPDQLFSAYMNAWKMNVIAALPLQLIIVGPLSRSVLKAIQSNE"
     gene            complement(11333..11848)
                     /locus_tag="U2K71_003024"
     CDS             complement(11333..11848)
                     /locus_tag="U2K71_003024"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629798.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HEM7317013.1"
                     /translation="MNTLAYILLCVLLSKPRSGYELKQLITIFWEAHHSQIYTTLAKL
                     DQQGYVTVLDKDEHSQKKIYHLTDEGRALVEEWIKEETPAPTQKDEFLAKIYAFSTLD
                     KTTATGLLFTREQQLNKIMQKNQKKLNDLSLDKKQEFGRYVVIQRRIMLCQQELQWCQ
                     QVREQMQQFFE"
     gene            11965..13167
                     /locus_tag="U2K71_003025"
     CDS             11965..13167
                     /locus_tag="U2K71_003025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002296013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HEM7317014.1"
                     /translation="MEILENQIDIQEQTNEETKGKSSALVVAATCMIAFMGVGLVDPI
                     LKTIAAQLNATPAETTLLFTSYMLVTGVVMLFTGFLSSRIGLKKTLMTGLFIIVVFAG
                     LGGLSGNIGMLIGLRAGWGVGNALFVSTALATIVSILVGNTEKAIMMYEAALGCGMAV
                     GPLVGGVLGSGSWRYPFFGVAFLMFCGFIALAVLLPETEKPKRKVGLWEGVKALSNHK
                     LRSVGLIALLYNFGFFTLLAYAPFLLIGYSELEVGFVFFGWGVLLAIASIFVAPALER
                     KFTTYRTILAAFVLFIICLLLLGAGTSMPVLVPISIVLAGFFQGIINTLLTTIAMEIP
                     GLQRNVASSSYSFVRFFGGALAPFIAGVIGEHIGESYAFYFAGAIVLISVCCIIYHRN
                     YFATNEEE"
BASE COUNT         4117 a         2427 c         2709 g         3965 t
ORIGIN      
        1 agaggttgta aaaaagctgt tctgcatcaa gtaataagaa gagtcaaata aaaaatagct
       61 cttcatatgt tacgtttggc tggacttatt accgcggagt tggcaggaga acgccgtttt
      121 tattcggaaa aaggagctga gacaagattt ttgtctcagc tcctgtttgt ttatcgataa
      181 aaaagtctgg ggaagacgat ggctcaggct ttgttggaaa cagagccgat tttatcattt
      241 gcttttacat cacgtgcggg ttcgatttct aagctttgga ctttgtcgcc atttgtgaat
      301 acaacgacca tatccgtgtt tttgcctgca gattgaatag cggcaagatc aactaaagca
      361 atcaattgac ctcttgcgac tttttgacct tcttttacat ataaagtgaa tggttcgcct
      421 tttaagtcaa cagtgttgat gcccatatga agcaagactt cgataccagc atctgtttgg
      481 attccgacag catgtttcgt tgggaaaatg tttgtgatcg taccagcgat tggagagaag
      541 atttctccat tttctggtaa tacagcatag ccgtcaccca tcattttttc cgcgaataca
      601 tcatctgaaa cttcagtgat tggaatcagt ttcccatttg cgatagcgta aatatcttgt
      661 tcgatcgttc caccggcaaa tgtttctgtt ttaaccacgg tgtttgtttg tgcaggtcgt
      721 gttgcagcac caccatgtag ttttgccatc tcatctgcaa cgaattgtac ttctgtacct
      781 acgatgactt gcgcattttt tccgtcgatg atcttcatac caggtacgcc agtggctttg
      841 attttatttt gatcgatcag gcctgtatct ttgacttgca aacgaaggcg tgtagtacag
      901 ttatcgatcg aagttacatt gtcggctccg cctaatgcag cgtagatttt gcttgcttgt
      961 gccgcatatt tattgtctga attagtcgtt gcagtatcgt catctgaaag atctgcttcc
     1021 tcgcggccag gagtcatcag atggaatttt ttgatagcaa agttaaagcc aaagtagtaa
     1081 atcactgctg ttactaatcc ttggacgatc aacatcaatg gctgattcgc aatcggattt
     1141 ttcaaagata ggaagtaatc gacaaatccg gcactaaagg caaatcctgc tgtccaatgg
     1201 aagaatgcac tcactgctaa agaaatccca gtgaaaattg catgcaagac atataatggc
     1261 caagcaacga acataaatga gaattcaagt ggttctgtta caccagtaaa gaatgaagca
     1321 aaagctgctg ctaacatcaa ggaagctgtt tttgctttgt tttctggacg tgcatttctg
     1381 taaatagcat atgctcctgc tggaagcccg aacatcatga ttgggaaaaa tccagcttga
     1441 tacattcctg ttacgccttt tgttccttca gaagccaaaa atttaccaat atcattgatt
     1501 cctgctacgt caaaccagaa cacggagttc attgcttgat gcaatcctgt agggatcagt
     1561 aaacggttga agaagccata aagacctgca ccaccccagc ctaaactcaa aatgaattta
     1621 gcaaaagaaa ctaaagcaga gtaaaccgga ggccatacga gcagtagcac tgcactgatc
     1681 agcaacatga caaaggcagc caagattggc actagacgct tgccgctaaa gaaagataaa
     1741 gccatcggta attttgcttc gtggaaacga ttataaagag ctgccgcaat caatccggca
     1801 ataataccaa taaatacgtt actgatctga gcaaaagatg gatcaactga ttctggtttg
     1861 atattcaata aagtagctac tgaatcagtt tttaaaacat ttactggaac ttcaaatgcg
     1921 accaaacctg ccaaggcagc agcacctgat ttgtccactg acatccctag tgctaaccca
     1981 acggcaaaca gcaaaggtaa ttgacctaaa actgcgttac cgccagcttg taaaaaatga
     2041 gcgacaatgt cgttactata agatgcccac cagtttccga tccctactag taaagcagca
     2101 gcaggtaaga ccgcaactgg aagttgaagg gaacgtccca ttcgttgtag atatgctttc
     2161 aatttttttc ctcctcatga ttgttaacat ttgtttgtac gattgcgctt cttaaacgct
     2221 cgatatggat ggccaagtaa cccagttctt ctttcgtgat cgcgagctgg tattctttgg
     2281 tcagcaacac acggattttt ttagaaatct tatagctttc tgggtatttt cgtttgacca
     2341 tttctacgat ttccgtgtct aaaacagcat attgttgcgc atgggttcgt tgtagaagca
     2401 gtcgtaaatg attcaccagt cttgaataat tcagtgaaaa tggttctgaa tggatatctg
     2461 tttccaattc tgattccact aaatggatga tgtcagatac aatcgtcact tcacggatac
     2521 tgcgatggtt attgttccga ccgcttcgtc cgctgtgaat atggattgcg atatatcctg
     2581 cttcgtcata agtatatgga atcccaagct cttttgtcag atactctact gcccattgag
     2641 caatactgaa ttcatcactg taaagcactt cgatttctct taaaagtttg ttgcggatga
     2701 cgataccgtt attaatgttc tccgcagaaa aagcaatgtg gtcggtcaaa gcgatcaata
     2761 catgttcatt caacttttcc atcaaaacag tttccgcatg atcaatgatc gtttctgctg
     2821 caaataaaaa gcgttcatca atctgattaa gtaaattttg caacttgatt tgaccttctg
     2881 gttccatgac aaatatccgt tcgacatcac gatcaaaaat caaatcattt ctttttctgt
     2941 caaatccaat cccttttcca atagctactt tttcctgtcc accgtcatct accaatactg
     3001 cattttgatt aaagatcttt ttgattttca tgcgcgcacc ctttttgaag atttggtttt
     3061 cacgtttgct agaccagttg attgcggcca aacaaaaata gacacgaaaa acctcctagg
     3121 gaaaccatgt tcctcagaga tttttcatgc ctagtatgat ctagtcacat gaaacgaatt
     3181 attgttaacg taatcaattt tatatagaca gttatgtttt gtcaattctt tttttataat
     3241 tatagtaatt tactggaatt atcagaaata aaaggaagga acaaattaag aaacttatgt
     3301 tatttaatca ttggatcttt tgcttaactt attcgttgtt aaaaagctga aagcgaagga
     3361 aaaaaactct acttaactgt atttctccgt taggacatct aaaaatttac gagcagcagg
     3421 agaaagaggc tggtcttttt tccaaatgag attcagtgaa gcttgtaatg gcggatcgag
     3481 tgggatgaat actttttccg tcccataagt attgatgatc ccatctagag ctaatgcacc
     3541 aaaagtcccg ttatcgacca tcaaagaagc attgtacaat aagttatatg ttccgacgat
     3601 cgataactga tctatgctga aacctagcca ttcagctagt tgactgtcca ctgacgtttg
     3661 ccgggaaatg atcaaaggaa cagttttcag ttcctgcgga tggatgactg tttgctttgc
     3721 taaaggatga ttccgatgta tcaaaagtcc ccaacgatct tgggctggca gtcgaaatga
     3781 ctggtatttt tgtttgtttg tcggttcgat tacgagtcca aaatcaagta atccgctatc
     3841 cagtttgtct atgatatcat ctgcattccc gctatataaa tgaaactgga tatcaggatg
     3901 cgccttgatc aacgaatgaa ggctatcaga aatgaaagag attgctttag tttcgcctcc
     3961 gccaatgtag atttctccag agatgattgc gtcatttgtt aaattgttcg ccgttttttc
     4021 taccaacgac aagatttctt ttccttttga ataaagatat cgaccagaat ccgtcaactg
     4081 tatctctcgg gagcctcgag tgaaaagtgt cacaccaagt tcttcttcta aatctttcag
     4141 ttgttttgag agagtaggtt gcgtgatatg cagagatttt gctgctcggc tgatattttt
     4201 ttctctagct actgctaaaa aatacgtcag tatacgaata tccatttttt ccctcctgtt
     4261 tttaaaaaag cataccacta agtatagtta aaaggaatag ttaagtatgt tttataagta
     4321 ttagacatca taagccgtcc gttataaagt aaggataaaa ggagggagca aaatgacaag
     4381 cctcatcgaa aaacaaatcg tcaataaaga gattcgagta acggatccgc tctttgaagt
     4441 gatccatcag atccaagcgg aaaacgaaga gcctttagca gcgctgaata ctgggtacca
     4501 tgaaccagca gatatccgca aacggctaga aaatatcatt agtgacaaag tagatgatac
     4561 ggtgactgtt ctgctgcctt tttatacaga tttcggcaaa catattagta tagggaaaaa
     4621 tgtctttatc aatcgtcaag tgatgttcgt tgacttaggt ggcatctgtt tagaagattc
     4681 agtattgatt ggtcctcgtg tcaacctcat cacggtgaac catctaaccg atccaaaaga
     4741 acgaagaggc ttgtcagtaa agccaatcca catcaagaaa aatgcttgga tcggagcagg
     4801 agcaactatt ctgccaggcg taacgattgg cgagaatgcc attgttgcag cgaatgcaac
     4861 tgttacgaaa gatgtcccag acaataccat cgttgcagga atccctgcca aaatcgtcaa
     4921 acctgtcgaa cggattattt gaacagtaag atgaaataag agatagtgag tataagcaag
     4981 agatttttca aaacaggagg agaatagaat gaatgattta tcagaaaaag tgattgtcat
     5041 catgggcgct tcaagcggaa tcggtgaagc aacaacgaag ctattagcag aaaaaggggc
     5101 taaactagtt atagctgcac gccgagaaga tcgcctaaaa gcaataaaag aatctttacc
     5161 agaagctgag ctatatattc aaacagcaga tgtaagagat tttgcacaag tccaagcagt
     5221 tatcgattta gcgatggaaa agttcggtcg aatcgatgta ttgtacaata atgcaggaat
     5281 catgccaacc gctccattag ttgaaggtca ccgtgatgaa tggcaaaata tgcttgatat
     5341 caacatcatg ggtgtgttaa atggaatttc cgccgtactt ccaatcatgg aaaaacaaaa
     5401 atctgggcat atcatttcaa ctgattctgt ggcgggacat gttgtttatc ctgattccgc
     5461 cgtttattgc ggaacaaaat ttgctgtccg tgcaatcatg gaaggattac gtcaagagca
     5521 acggcaaaat aacatcaaat ctacgatcat ctctccagga gctgttcaaa ctgaattata
     5581 tcaaaccatc agcgacaaaa aggctgctct tgaacttcat gaagcgcaaa aagaatgggg
     5641 attaacatct gaagatatag cttctgctgt agcatttgcg atagaaacgc cagaccggat
     5701 gtctgtcagc aacatgatta tccgtccgac gacacaggaa gtataatcca taacggataa
     5761 tatccagaga gaattacttg ttaaatagtt attcaaacga ccatcaattt aaggcgtgaa
     5821 tggagaagaa tattcttcat ttacgtcttt ttatgcagaa aaagcaaatt tattaaaaag
     5881 aaacaaatgt tatttaaata tggacattga cttgctttta cttttttagt tacactgtta
     5941 tttaggaaaa gtttcataat tcaaaggaga aaaaagatga aagcaaagaa tttattattt
     6001 agcgttattc ttttaggaaa tctagcagtt gccaacacaa cagtgctagc tgaagaatcg
     6061 acagaaactg tcgcaactca gacatcgagt tcttcagaaa caggaattga aaacgataca
     6121 gaaagtactt tgcagtcagc tactgtctca gaaaatacaa tttcaaccga agaaccaaca
     6181 actgaaacaa cagaacaaac acaaaccgaa gacagtacat cagcagaagc tcagaacaat
     6241 ccagagaatg cagaagaaat cacgaaccaa ttgacagatg ggcaaacatt gacacatacc
     6301 catcaagcag atttgtctga attcgaaaac tatactggct atgcttttta tgctcgctca
     6361 tctgctaatt ctcaacaagc gttcattgat agtattgcct caactgcaca aagtttggca
     6421 agtgccaatg acttatatgc atcagtcatg attgctcaag ccatcgttga aagtggctgg
     6481 gggaacagta ctttggcttc tgcccctaat tacaatctat ttgggattaa aggaagctat
     6541 aatggccaat ctgttacgat gcctacttcg gaatatgtga atgggcaatg gattacggtg
     6601 aatgccgctt ttagaaaata tccttcgtac aaagaatcat tgcaagataa cgtaacggta
     6661 ttgaagacga cttcttttca gccaggcgtt tactactatt ccggtgcatg gaaaagcaat
     6721 acgaactctt acaaagatgc tacggcttgg ctaacaggtc gttatgccac tgcgcctaat
     6781 tatggttcta cattgaacaa tgtaatcgaa acctataatc tgacacagta tgatacagca
     6841 ccaacttcaa cttctccgat gtatcgtctg tataaccgtc atacaggaga acatttatat
     6901 acattgaatg ccggagaaaa aaattatctg ccaactgtag gctgggaata tgaagggatt
     6961 gcttggcaag caccaaacag cggacaacct gtctaccgtt tatacaatcc ttacagtgga
     7021 gaccatcatt acacaatggc acaatctgag atcaactctt taacaaaaat cggttggcgt
     7081 tatgaaggtc taagttttta ttctggtggt tccaagccaa tttatcgact atttaatcca
     7141 aatgaaaaaa ctggtaccca tcactatacc ttgagtgcta aggaaagaga ttggctaaca
     7201 ccgatgggct ggagatatga aggaatagga ttttatggtt attaaaaaga ggtagtgaaa
     7261 aagctgccct gcatcaagta gtaagaacag ccaaacaaaa acagattttc atgttttttg
     7321 tttggttgaa cttattaccg cagatgcaag tttttgaaca ccgtttactc aaaaaaggtt
     7381 gtgacaacat cttgtcataa cttttttatt actgtttaag catactgatc caatttctaa
     7441 tattcgacgg gattcttgca aaaggcgatt tcggaaattt ttggcggtgt aaaattatct
     7501 actgcagaag agctatcttc tatagtttga taagaatcaa taatcttggt tttagcgttg
     7561 tgctttcttt atcatttttt ccgttcattc tacaaaaaat ttcttttctc tatacttttc
     7621 tattgagagc gtttttttta ttacacagct gattctttga cttttttctt tttttattct
     7681 attattgaca agtcaatcgt tgacgagtca attattagaa aggaggaaat tatgtcttta
     7741 gaagagatta gtcacctttt atatcaaatc aaattagccg atcaaaaaat cgtccagatc
     7801 tttgaagaaa atatcggtat aagtcttact agatacgaga tcatgatgag actgaaagaa
     7861 gaaaatccat tgctacaaag cagtttgcaa gaaaagttgc agattgatca aggagcagtt
     7921 actcgtcatc tgaaggtatt ggaagcaaat ggctatgttt cgcgtcagag aaatcctcag
     7981 aacaaccggg aagtgtatgt agcgctaaca gaaaaagccc agaagaaaat caagcattgt
     8041 gaaatggacc ataaagatac aggttccgtg ctatccccgg ctttcagcga tgtagaaatg
     8101 aaacaatttc taacactttt agataaattg aatacccaac tttgcaatat aacaacagaa
     8161 acgaaagagg ttaataataa tggaacaaaa aaatgatttt tcaacaatta tgactggtag
     8221 aagatccgtt cgcgtatatg atgaaacata caaaatccca cacgaagaaa tgctggaaat
     8281 gatcaaagaa gcgacgactg caccttcttc tgtcaatatg aaaccatggc gctttgtggt
     8341 cgctgaaagc gaggaagcaa aagaaatctt gcgtcctttg atccgtttca atacgagaca
     8401 aaatgatact tcttctgcaa tggtcatgat ttttggcgat atgcaatgtt acgaatatgg
     8461 cgaagaaatc tacaaccaag cttacgaatc agggaagatg ccaaaagaag tcagagatca
     8521 acaattagct gcgatcattc cttattacaa gagtttttca agagaagaaa tgaacgatgt
     8581 agtcaaagtc gattctagct tagcggctat gcaattcatg ttagttgcgc gtgatcatgg
     8641 ctatgaaaca aatccaattg gtggattcga agcagatcag ttggcagagg catttggttt
     8701 ggataaagac cgttatgttc cagttattat tttatcaata ggaaaagcgg tagaagaagg
     8761 ttacgagtct gttcgtttag caccagaaaa gatcactatg ttcgaataaa aggttttcag
     8821 ttactattca gtagctattg ctttttttga atcgtgtgtg gtaacctaat aaaaaaggag
     8881 gtcggaaaaa tgactgcatc gatgaaatta cgtttcgaac ttttgaataa caactaaata
     8941 aattcaaaag acatgtataa acaacaattt catggatgga gtatgtccat tttttcgaac
     9001 gacagtagaa taggtgaaag ctatgacaga tttggtgatt tagtcatagt tatttcgttc
     9061 agagtaggaa aagatgtgat gcagtttttt tgcaaacaga tttttctgct cttttttagt
     9121 atcgataatt gttgttttac atgaaatagg agtgtaaaac atatggaaaa tttagcagta
     9181 aatataacaa atctacaagt cagctttggc aaccagctag aactatctat tgattctctt
     9241 cgtgtctatc agcaagatcg tatagggatc ataggagaaa atggtgtcgg taaatcgact
     9301 ttgctcaaac taatagccgg tgaacttttt cccgatcatg ggaaaatcca aacagagatc
     9361 accttcaact acctgcctca attaacctat ctcgctgaag caaaggacct aaatttggaa
     9421 ttagccagtc atttccagtt aagactggaa gaaacttcag agcggaaatg gagcggaggg
     9481 gaagaacgaa agatcgagtt gatacgtctt ctttcttctt atgaacaagg gatgcttcta
     9541 gacgagccaa caacccatct agatagaaaa agtattgatc gactgattga agagcttcgt
     9601 tattattatg gtacgctggt ttttgttagt catgatcgct attttctaga tgagctagca
     9661 tcgaaaatct gggaagtaaa agacggagaa atccgagagt tttcggggaa ttatagtgcc
     9721 tatctcactc aaaaggaatt ggagaaaaag actcagctac gagaagcaga gtcgatcatg
     9781 aaagagaaaa aacgattgga aaaatcgatc caagaaaaga aaaaacaagc ggaaaagttg
     9841 gaaaaagtgt ccagtaaaaa gaaaaagcaa caaatcagac tggatcggtt gtcttcctct
     9901 aaacaaaaag acagtgtaca aaaagccatc caaaagaatg cgaaaacatt agagagaaga
     9961 ctccaaaaaa taggagaaac aaccaagccg cagcagatga aacaaatccg ttttccagta
    10021 ccaaaatctc ttgaactcca cagccgctat cccatcatgg gacaaaatgt ccaattggaa
    10081 agaagtggga gaacattact ggtaaatggt gattttcagt tttctttagg taaaaaaatc
    10141 gcgattgtcg gtgaaaatgg ttcaggtaag acaaccttat tagaacatat ccgcaaacaa
    10201 ggagaaggaa tccttctctc tccgaaagta agctttcaag tatatcagca aaagggttat
    10261 caaatgacat ctgaagaatc catcattcgt tttgtcatga gacaaacaga gttttcagaa
    10321 tcgcttgtcc gtagtttgct gaatcactta gggtttgctc aggaaacttt gacgaaaccg
    10381 ttatgtacat taagtggggg agaagcgacc cgtctgacga ttgctttgct ttttactaag
    10441 ccaagtaatg tgttgctgtt agatgaaccg actaatttta ttgatatggc aacgatcgaa
    10501 gctttagaga agctaatgca aatatatccg ggaacgattt tgtttacttc tcatgattct
    10561 tactttgtcg agcgtacggc tgatgaagtt tatgaaataa aagggcagaa aataaaaaaa
    10621 gtacttacga gaaattttta aatgtgctac gatgattttc gtgacaaatt taaaggagtg
    10681 gtacagattg cctacaaata aaaaagaagg aataattttt acaacaatga tgtgtttttt
    10741 gatggtcttg gggatgagcg cgtataattt atggctgcat caagatcttc atttatcaag
    10801 tttgttgatt ggtctagtgc ctggattcgt ggtggctttc attttggatg tcttcatcat
    10861 cggtgtgctg gcgaaaaaaa ttgcctttaa actaccagta aatccagaaa gcaaggtcca
    10921 aatgatctta actatttcat gcttgatggt tttaggaatg gtgacatgta tgtctttatt
    10981 cggtgtgatc atggaacaag gaatacctga tcaattattc tctgcttata tgaatgcttg
    11041 gaaaatgaat gtgattgccg ctttaccgtt gcagttgatc atcgttggcc cgctttcaag
    11101 aagtgtgcta aaagcaattc aaagtaatga atgatggaac actgttattt gaaaaaaggc
    11161 cttggcatgc tttttgtcat agtctcttga atgcagttct agaaaaatag tgatccgaac
    11221 gataggtttt ggcctgtcgt tcggatcgct ttttcttttc caactgtatt gctcttttat
    11281 tgaagataat ttgctcttca atagatattt ctagaaaggt tctgtttatc ccttactcaa
    11341 aaaactgctg catttgttct cttacttgct gacaccattg gagttcctgc tgacaaagca
    11401 taattctgcg ctggatcaca acataacgtc caaattcttg tttcttgtct agcgagaggt
    11461 cattcaattt tttttgattt ttttgcatga ttttgttcag ctgttgttct ctagtgaata
    11521 gcagtccagt agctgttgtt ttatcgagcg tcgaaaaagc atagattttt gctagaaatt
    11581 catctttttg cgtaggagcg ggagtttcct ctttgatcca ttcctctaca agagcacgtc
    11641 cttcatcagt caagtggtag atcttttttt gagagtgctc gtctttgtcc aaaacggtca
    11701 cataaccctg ctggtctaac ttggcaagcg ttgtatagat ctgactgtga tgggcttccc
    11761 aaaaaattgt gatcagctgc ttcaattcat acccagaacg tggtttggat aaaagcacgc
    11821 ataataatat ataggctaaa gtattcataa aaatcctacc tctcgtctta ttttctttat
    11881 tatatcacag taaagtttcc acgttttaca tgtgcatatt gacatgtaaa ataaatcatg
    11941 tatgatgcta atagagagaa agggatggaa atattggaga atcaaataga tatacaagaa
    12001 caaacaaatg aagaaacaaa ggggaaaagt tcagcgctag ttgtagcagc tacctgtatg
    12061 atcgctttta tgggagtggg tctagtagac ccaatcttaa aaacgattgc tgcacagtta
    12121 aacgctacgc cagctgaaac gaccctgtta tttactagtt acatgttagt cactggtgtg
    12181 gtgatgctat ttacaggttt cttatctagt cggatcggtt tgaaaaaaac attgatgaca
    12241 ggattgttta tcatcgttgt attcgctggt cttggcggat tatctgggaa tatcggaatg
    12301 ctgattgggt tacgtgccgg ctggggtgtc gggaatgctt tatttgtctc tactgcttta
    12361 gcaacgatcg tcagtatttt agttggtaac acagaaaaag caatcatgat gtatgaagca
    12421 gcacttggct gcggaatggc tgttggccct ctagtcggtg gggtcttggg cagtggttca
    12481 tggcgttatc cattttttgg ggtagcattt ttgatgtttt gcggttttat agccctagcg
    12541 gtacttttac ctgaaactga gaaaccaaag agaaaagtag gcctttggga aggtgtcaaa
    12601 gcgttaagca atcataaact tcgttcagtt ggactaatcg cattgttata taatttcgga
    12661 ttctttactt tacttgcgta tgcgccattt ttattgattg gctattctga attagaagtc
    12721 ggttttgtat tttttggctg gggtgtgctt ctggctattg cttcgatttt cgttgcaccg
    12781 gctcttgagc ggaagtttac gacttatcgg acaattttag cagcatttgt cttattcatt
    12841 atttgtttgc tgttattagg agctggaact tctatgccag tccttgtacc gatcagcatt
    12901 gtgttagcag gatttttcca aggaattatc aatacactgt tgacgacgat tgctatggaa
    12961 ataccaggtc tgcaacgaaa cgttgcttcg tccagctata gttttgtccg ctttttcggt
    13021 ggagctttag caccatttat tgcaggtgtg attggtgaac atattggtga aagctatgcg
    13081 ttttattttg caggtgccat tgttcttatc agtgtatgtt gtatcattta tcaccgtaat
    13141 tattttgcta caaatgaaga agaataagaa atcagaggtt gtgaaaaagc tgtcctgcat
    13201 caagtcataa gaacagcc
//
LOCUS       DASHHB010000209        11821 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.078, whole genome shotgun sequence.
ACCESSION   DASHHB010000209 DASHHB010000000
VERSION     DASHHB010000209.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 11821)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11821)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11821
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.078"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            39..977
                     /locus_tag="U2K71_003026"
                     /pseudo
     CDS             39..977
                     /locus_tag="U2K71_003026"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000331151.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
     gene            980..3163
                     /locus_tag="U2K71_003027"
     CDS             980..3163
                     /locus_tag="U2K71_003027"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017646277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HEM7317015.1"
                     /translation="MKPSILNRIKSNWTLKRLGKVAMTVAFTLVIAIFLLAMLGTVVQ
                     AAGLVDDTVNVANEYSRYPLENYQLDFYVDNSWGWLPWNWSDGIGKQVMYGLYAITNF
                     IWTISLYVSNATGYLVQEAYSLDFISATADSIGKNMQTLAGVTPSGLSTEGFYVGFLL
                     LLILVLGVYVAYTGLIKRETTKAIHALMNFVLVFILSASFIAYAPDYIKKINDFSSDI
                     SNASLSLGTKIVMPHSDSQGKDSVDLIRDSLFSIQVQQPWLLLQYNSSDIESIGIDRV
                     ESLLSTSPDSNNGEDREKIVAEEIEDRSNTNMTITKTINRLGTVFFLFVFNIGISIFV
                     FLLTGIMIFSQVLFIIYAMFLPVSFILSMIPSFDGMSKRAITKLFNTILTRAGITLII
                     TTAFSISTMLYTLSAGYPFFLIAFLQIVTFAGIYFKLGDLMSMFSLQSNDSQSVGSRM
                     MRKPRMLMHAHMHRLQRKLGRSVTALGAGSAIASATGKKGQSGSGSSASTQADHSRPN
                     GQEKSTLGKRIGQTIGTVADTKDRMVDTAGNLKEQVKDLPTNARYAVYQGKSKVTDNV
                     HDLTRSISQTKADKASGRKKQQEQRRKTIAERRSEMEQVKQKKQPASSVHERPATKQE
                     QSHDGQTSRQTTVQASYRESQQAKQERPTVKSDSSSLKTERQSNTVQERTVQKPVTST
                     APTDRASQRSITKERQSTVQRVPLQNTKSRPPIKTATIKKGSKKP"
     gene            3160..4161
                     /locus_tag="U2K71_003028"
     CDS             3160..4161
                     /locus_tag="U2K71_003028"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018368130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional lysozyme/C40 family peptidase"
                     /protein_id="HEM7317016.1"
                     /translation="MKLKTLVIGGSGLFLMVFSLLLFVAILFSDEQDGGFSNIHYGGV
                     DVSAEVLAHKPMVEKVAKEYGIEEYVNILLAIIQVESGGTAEDVMQSSESLGLPPNSL
                     STEESIKQGVKYFSELLASSERLGVDLESVIQSYNYGGGFLGYVANRGNKYTFELAQS
                     FSKEYSGDEKVSYPNPIAIPINGGWRYNYGNMFYVQLVTQYLVTTEFDDDTVQAIMDE
                     ALKFEGWKYVYGGASPTTSFDCSGLTQWTYGKAGINLPRTAQQQYDVTRHIPLLEAKA
                     GDLVFFHSTYNAGSYITHIGIYLGDNRMFHAGDPIGYADLTSSYWQQHLVGAGRIKQ"
     gene            4176..5090
                     /locus_tag="U2K71_003029"
     CDS             4176..5090
                     /locus_tag="U2K71_003029"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002345006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="conjugal transfer protein"
                     /protein_id="HEM7317017.1"
                     /translation="MMKFGKNQNTEKQTPKEKKPRVYKVNPRKKVVIVLWVLLGLSFS
                     FAIFKHFTAIDTHTIHETTIIEKEYVDTHHVENFVENFAKVYYSWELNDKSIDNRIEN
                     LKAYLTEELQALNVDTVRKDIPVSSSVKGFQIWTVEADGDNQFDVTYSVDQLITEGEN
                     TKTVHSAYIVSVYVDSTGNMVLIKNPTITSIPKKSDYKPKAIESEGTVDSITTNEINE
                     FLTTFFKLYPTATASELSYYVNDGILKPIGKEYIFQELVNPIYNRKDNQVTVSLTVEY
                     IDQQTKATQVSQFDLVLEKNGSNWKIIE"
     gene            5335..5451
                     /locus_tag="U2K71_003030"
     CDS             5335..5451
                     /locus_tag="U2K71_003030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance determinant leader
                     peptide"
                     /protein_id="HEM7317018.1"
                     /translation="MILKYPENICMLCMPMVMHKNPSDKSIYHWDFYALLGF"
     gene            5467..7386
                     /gene="tet(M)"
                     /locus_tag="U2K71_003031"
     CDS             5467..7386
                     /gene="tet(M)"
                     /locus_tag="U2K71_003031"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011117014.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline resistance ribosomal protection
                     protein Tet(M)"
                     /protein_id="HEM7317019.1"
                     /translation="MKIINIGVLAHVDAGKTTLTESLLYNSGAITELGSVDKGTTRTD
                     NTLLERQRGITIQTGITSFQWENTKVNIIDTPGHMDFLAEVYRSLSVLDGAILLISAK
                     DGVQAQTRILFHALRKMGIPTIFFINKIDQNGIDLSTVYQDIKEKLSAEIVIKQKVEL
                     YPNMCVTNFTESEQWDTVIEGNDDLLEKYMSGKSLEALELEQEESIRFQNCSLYPVYH
                     GSAKSNIGIEQLIEVITNKFYSSTYRKKSELCGNVFKIEYSEERQRLAYVRLYGGILH
                     LRDSVRISEKEKIKITEMYTSINGELCKIDKAYSGEIVILQNEFLKLNSVLGDTKLLP
                     QRERIENPLPLLQTTVEPSKPQQREMLLDALLEISDSDPLLQYYVDSTTHEIILSFLG
                     KVQREVISALLQEKYHVEIELKEPTVIYMERPLKNAEYTIHIEVPPNPFWASIGLSVS
                     PLPLGSGMQYESSVSLGYLNQSFQNAVMEGIRYGCEQGLYGWNVTDCKICFKYGLYYS
                     PVSTPADFRMLAPIVLEQVLKKAGTELLEPYLSFKIYAPQEYLSRAYNDAPKYCANIV
                     DTQLKNNEVILSGEIPARCIQEYRSDLTFFTNGRSVCLTELKGYHVTTGEPVCQPRRP
                     NSRIDKVRYMFNKIT"
     gene            7484..8857
                     /gene="tet(L)"
                     /locus_tag="U2K71_003032"
     CDS             7484..8857
                     /gene="tet(L)"
                     /locus_tag="U2K71_003032"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002387933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetracycline efflux MFS transporter Tet(L)"
                     /protein_id="HEM7317020.1"
                     /translation="MKCNECNRVKYNQILIWLCVLSFFSVLNEMVLNVSLPDIANDFN
                     KPPASTNWVNTAFMLTFSIGTAVYGKLSDQLGIKRLLLFGIIINCFGSVIGFVGHSFF
                     SLLIMARFIQGAGAAAFPALVMVVVARYIPKENRGKAFGLIGSIVAMGEGVGPAIGGM
                     IAHYIHWSYLLLIPMITIITVPFLMKLLKKEVRIKGHFDIKGIILMSVGIVFFMLFTT
                     SYSISFLIVSVLSFLIFVKHIRKVTDPFVDPGLGKNIPFMIGVLCGGIIFGTVAGFVS
                     MVPYMMKDVHQLSTAEIGSVIIFPGTMSVIIFGYIGGILVDRRGPLYVLNIGVTFLSV
                     SFLTASFLLETTSWFMTIIIVFVLGGLSFTKTVISTIVSSSLKQQEAGAGMSLLNFTS
                     FLSEGTGIAIVGGLLSIPLLDQRLLPMEVDQSTYLYSNLLLLFSGIIVISWLVTLNVY
                     KHSQRDF"
     gene            9421..10683
                     /locus_tag="U2K71_003033"
     CDS             9421..10683
                     /locus_tag="U2K71_003033"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001619005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid recombination protein"
                     /protein_id="HEM7317021.1"
                     /translation="MSYAVCRMQKVKSAGLKGMQFHNQRERKSRTNDDIDHERTRENY
                     DLKNDKNIDYNERVKEIIESQKTGTRKTRKDAVLVNELLVTSDRDFFEQLDPGEQKRF
                     FEESYKLFSERYGKQNIAYATVHNDEQTPHMHLGVVPMRDGKLQGKNVFNRQELLWLQ
                     DKFPEHMKKQGFELKRGERGSDRKHIETAKFKKQTLEKEIDFLEKNLAVKKDEWTAYS
                     DKVKSDLEVPAKRHMKSVEVPTGEKSMFGLGKEIMKTEKKPTKNVVISERDYKNLVTA
                     ARDNDRLKQHVRNLMSTDMAREYKKLSKEHGQVKEKYSGLVERFNENVNDYNELLEEN
                     KSLKSKISDLKRDVSLIYESTKEFLKERTDGLKAFKNVFKGFVDKVKDKTAQFQEKHD
                     LEPKKNEFELTHNREVKKERSRDQGMSL"
     gene            10928..11815
                     /locus_tag="U2K71_003034"
     CDS             10928..11815
                     /locus_tag="U2K71_003034"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001611915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein rep"
                     /protein_id="HEM7317022.1"
                     /translation="MCPNSSIYSDEKSRVLVDKTKSGKVRPWREKKIANVDYFELLHI
                     LEFKKAERVKDCAEILEYKQNRETGERKLYRVWFCKSRLCPMCNWRRAMKHGIQSQKV
                     VAEVIKQKPTVRWLFLTLTVKNVYDGEELNKSLSDMAQRFRRMMQYKKINKNLVGFMR
                     ATEVTINNKDNSYNQHMHVLVCVEPTYFKNTENYVNQKQWIQFWKKAMKLDYDPNVKV
                     QMIRPKNKYKSDIQSAIDETAKYPVKDTDFMTDDEEKNLKRLSDLEEGLHRKRLISYG
                     GLLKEIHKKWFCCKVLNKE"
BASE COUNT         3932 a         1958 c         2463 g         3468 t
ORIGIN      
        1 acttggagat aacatcaaaa agttacctat cgctataacg tggcagatgg aaagaaacct
       61 tgccagagat ttcccatgaa atcaatatcg tcaatctgac ttctgatgag aaaaacaaag
      121 gcttacttga cccttatgta attatgaaaa ataccaaaga ttctgaatca ctggctattg
      181 atattttgac attccttacg gggatttcct ctcgtgatgg ggaacgcttc ccaatcctta
      241 gaaaagccat tcgtgcagta accaatagtg aagtgcgagg gttgatgaaa gtgattgaag
      301 aattacgggt tgagaatacg ccactaagta ccagcatagc cgaccatatc gaaagtttta
      361 cagactatga ctttgcccat ctgcttttta gtgatggtta tgtagagcag tctatcagcc
      421 ttgaaaaaca actgaacatt atacaggttg ccgacttggt acttcctgac aaggaaactt
      481 cctttgagga atataccaca atggagttac tatcggtagc aatgctgatt gtcattagta
      541 cctttgcgtt ggactttatc catacagacc gaagtatttt caagattgta gacttagacg
      601 aagcatggag cttcttacag gtggcacaag ggaaaacact atctatgaag ctggttcgtg
      661 ctggtcgtgc tatgaacgct ggggtatatt tcgtgaccca aaatacagat gacctcttag
      721 atgaaaaact gaaaaataac ctcggcttaa aatttgcctt tcgttccact gaccttaacg
      781 agattaaaaa aacccttgcc ttttttggtg tagacccaga ggacgaaaac aatcagaagc
      841 gattgcgtga tttggaaaac gggcaatgcc ttatcagtga tttatatggt cgtgtcggtg
      901 tgatacagtt ccaccctgta tttgaagaac tgctccatgc ctttgatacc agaccacctg
      961 tgcgaaaaga ggtgtaaatg tgaaaccatc aatattaaac agaataaaat caaactggac
     1021 gctgaaacgt ctaggtaaag tggcaatgac agtggctttc acacttgtga ttgccatttt
     1081 tcttttagcc atgctgggaa cggtggttca agctgcgggc ttggtagatg atacggtcaa
     1141 tgtggcaaat gaatacagcc gatacccact tgaaaactat caactggatt tttatgtgga
     1201 taatagctgg ggctggctac cgtggaactg gtcggacgga attggaaaac aagtcatgta
     1261 tggactatat gccattacca attttatttg gacaatcagt ctttatgttt ccaatgcgac
     1321 gggttactta gtacaggaag cctattcctt agacttcatt tccgctacag cagattccat
     1381 tggcaagaat atgcagaccc ttgcaggggt tacgcctagt ggactatcaa cagagggttt
     1441 ctatgttgga ttccttttac tcttgatttt ggttcttggg gtttatgttg cctatacagg
     1501 actgataaag agagaaacca caaaggcaat tcatgccctc atgaattttg tgctggtttt
     1561 tatcctatcg gcttccttta ttgcctacgc tcccgactac attaaaaaaa tcaatgactt
     1621 ttcatcagac atcagcaacg ctagtttgtc actgggtaca aaaattgtca tgccccattc
     1681 agatagtcaa ggcaaggaca gtgtggactt gatacgtgac agcctatttt ccatacaggt
     1741 tcagcaaccg tggctactgc ttcaatataa cagttcagac attgaaagta ttggtattga
     1801 ccgtgtggaa agcctgctct caaccagtcc agattccaac aatggggaag acagagaaaa
     1861 aattgttgcg gaagaaattg aagatagaag caataccaat atgacgatta caaagacgat
     1921 taaccgttta ggtacagtct tcttcctatt tgtcttcaat attgggattt ccatatttgt
     1981 attcctatta acaggaatca tgattttctc gcaggtactt tttatcatct atgctatgtt
     2041 tctgcctgtg agctttattt taagcatgat tccatcattt gatggtatgt caaaacgagc
     2101 cataacaaag ctctttaata ccattttgac acgagctgga atcacattga ttattacgac
     2161 agcatttagt atttcaacca tgctctatac cttatcggct ggttatccgt tctttttgat
     2221 tgcttttcta cagattgtga cctttgcagg aatctacttt aagctgggcg atttaatgag
     2281 tatgttttct ctacagagta acgattctca aagtgtggga agtcgtatga tgagaaaacc
     2341 tcgtatgctt atgcacgctc acatgcaccg tctacagcgg aaacttggac gctccgtgac
     2401 tgctttaggg gctgggtctg ccattgccag tgctacgggt aaaaaaggac agtcgggttc
     2461 gggaagttct gcgagtacac aagcagatca ctctcgacca aacgggcagg aaaaatcaac
     2521 actaggaaag cgtatcggtc aaaccatcgg tacagtagct gataccaaag acagaatggt
     2581 agatactgct ggtaatttga aagaacaggt taaggatttg ccgaccaatg caagatatgc
     2641 agtatatcaa ggaaaatcta aagtgacgga taatgtccat gatttaacca gaagtatttc
     2701 tcaaaccaaa gcggataaag ccagtggacg caagaaacag caggaacaaa gacggaaaac
     2761 cattgcggag cgtcgctctg aaatggaaca ggtcaaacag aaaaaacagc cagcttcttc
     2821 cgttcatgaa agaccagcca ctaagcaaga acaatctcat gatggacaga cttcaaggca
     2881 aacaactgta caggcttcct atagggaatc tcaacaagcc aaacaagagc gtccaacagt
     2941 taagtccgat tcttcaagtt taaaaacgga acgtcaaagt aatacagttc aagaaagaac
     3001 ggttcaaaag ccagtaactt caactgcacc aacagataga gcttcacaac gttcaatcac
     3061 aaaagaacgt cagtctactg ttcaaagagt accactacaa aatacaaaaa gtagaccacc
     3121 aatcaaaacc gccaccatta agaaaggcag taagaaacca tgaagctgaa aactttagtg
     3181 attggtggtt cgggattatt cttgatggtc ttctcactgc ttctgtttgt cgccatttta
     3241 ttttcagatg aacaagatgg cggtttttcc aacattcact atggcggtgt ggatgtttcc
     3301 gcagaagtgc tggctcataa gcctatggta gaaaaagttg ccaaagaata tggaattgaa
     3361 gaatacgtca atatacttct tgcgattata caggtggaat cgggtggtac agcagaagat
     3421 gttatgcagt cctcggaatc cctcggtctt ccacctaatt cattgagtac agaagaatcc
     3481 attaagcaag gtgtgaagta tttcagtgag ttattagcca gtagcgaaag gcttggtgta
     3541 gatttagaat cggttatcca gtcctacaat tatggtggtg gtttcttagg atatgtggct
     3601 aatcgtggga ataaatatac ctttgaactg gctcaaagtt tctcaaaaga gtattcgggt
     3661 gacgaaaaag tgtcttaccc caatcccata gccataccta tcaatggggg ctggcgatac
     3721 aattacggga atatgtttta tgtgcaactg gtaacgcagt atcttgtcac aacagagttt
     3781 gatgatgata cagtacaagc catcatggac gaagctctga aatttgaagg ctggaagtat
     3841 gtctatggcg gagcttcccc gactacctct tttgattgta gcggactgac acaatggacg
     3901 tatggaaaag ctggaatcaa cttaccacga acagcacaac agcaatatga tgtaacccgg
     3961 catatcccat tattggaagc aaaagccggc gatttggttt tctttcattc tacctataac
     4021 gctggctctt atattactca tatcgggatt tatttaggcg ataaccgtat gtttcatgca
     4081 ggcgacccta tcggttatgc cgacttaaca agctcttact ggcaacagca tttagtggga
     4141 gcaggacgaa tcaaacaata agaaaggacg attcaatgat gaaattcgga aaaaatcaga
     4201 atacagaaaa acagacacca aaggaaaaga aacctcgtgt ctataaggtc aatcctcgta
     4261 aaaaggttgt gattgtccta tgggtacttt taggacttag tttcagcttt gcgatattca
     4321 agcactttac agccattgat acccatacca ttcatgaaac caccatcata gaaaaggaat
     4381 atgtcgatac tcatcatgtg gaaaattttg tagagaactt tgcgaaagtc tactattcat
     4441 gggaactaaa cgacaagtcc attgataatc gcatagaaaa cctgaaagca tatctgacag
     4501 aggaacttca agctctgaat gtcgatacag ttcgtaaaga tattcctgta tcgtcttctg
     4561 taaaaggatt tcagatatgg acggtagaag cagatggcga caatcaattt gatgtaacct
     4621 acagtgtaga ccaactcatt acagaggggg aaaatacaaa gaccgtccac tctgcttata
     4681 tagtgagtgt ctatgtagac agtactggaa atatggtcct cattaagaat ccgaccatta
     4741 ctagcatacc aaagaaatca gactataaac caaaagctat tgaaagtgag ggtacggttg
     4801 attccattac aaccaatgaa atcaatgagt ttttaacgac gttcttcaag ctctatccta
     4861 cagcgacagc cagtgaactt tcctactatg tgaatgacgg gatattaaaa ccaatcggaa
     4921 aagagtacat ctttcaagaa ctggtaaatc ctatttacaa tcgtaaggat aatcaagtca
     4981 cggtatcgct gacagtggag tatatcgacc agcagaccaa agcaacgcag gtatctcaat
     5041 ttgatttggt acttgaaaag aacgggagta attggaagat tatagaataa caaatattgg
     5101 tacattatta cagctatttt gtaatcacgt actctctttg ataaaaaatt ggagattcct
     5161 ttacaaatat gctcttacgt gctattattt aagtatctat ttaaaaggag ttaataaata
     5221 tgcggcaagg tattcttaaa taaactgtca atttgatagt gggaacaaat aattggatgt
     5281 ccttttttag gagggcttag ttttttgcac ccagtttaag aataccttta tcatgtgatt
     5341 ctaaagtatc cggagaatat ctgtatgctt tgtatgccta tggttatgca taaaaatccc
     5401 agtgataaga gtatttatca ctgggatttt tatgcccttt tgggcttttg aatggaggaa
     5461 aatcacatga aaattattaa tattggagtt ttagctcatg ttgatgcggg aaaaactacc
     5521 ttaacagaaa gcttattata taacagtgga gcgattacag aattaggaag cgtggacaaa
     5581 ggtacaacga ggacggataa tacgctttta gaacgtcaga gaggaattac aattcagaca
     5641 ggaataacct cttttcagtg ggaaaatacg aaggtgaaca tcatagacac gccaggacat
     5701 atggatttct tagcagaagt atatcgttca ttatcagttt tagatggggc aattctactg
     5761 atttctgcaa aagatggcgt acaagcacaa actcgtatat tatttcatgc acttaggaaa
     5821 atggggattc ccacaatctt ttttatcaat aagattgacc aaaatggaat tgatttatca
     5881 acggtttatc aggatattaa agagaaactt tctgccgaaa ttgtaatcaa acagaaggta
     5941 gaactgtatc ctaatatgtg tgtgacgaac tttaccgaat ctgaacaatg ggatacggta
     6001 atagagggaa acgatgacct tttagagaaa tatatgtccg gtaaatcatt agaagcattg
     6061 gaactcgaac aagaggaaag cataagattt cagaattgct ccttgtaccc tgtttatcat
     6121 ggaagcgcaa aaagcaacat agggattgag cagcttatag aagtgataac gaataaattt
     6181 tattcatcaa catacagaaa gaagtctgaa ctttgcggaa atgtcttcaa aattgaatat
     6241 tcggaagaaa gacaacgtct tgcatatgta cgcctttatg gcggaatcct gcatttgcgg
     6301 gattcggtta gaatatcgga aaaggaaaaa ataaaaatta cagaaatgta tacttcaata
     6361 aatggtgaat tatgtaaaat tgataaggct tattccgggg aaattgttat tttgcaaaat
     6421 gagtttttga agctaaatag tgttcttgga gatacaaagc tattgccaca gagagagaga
     6481 attgaaaatc cgctccctct gctgcaaaca actgttgaac cgagcaaacc tcaacaaagg
     6541 gaaatgttac ttgatgcact tttagaaatc tccgacagtg acccgcttct acaatattat
     6601 gtggattcta cgacacatga aattatactt tctttcttag ggaaagtaca aagggaagtg
     6661 attagtgcac tgttgcaaga aaagtatcat gtggagatag aactaaaaga gcctacagtc
     6721 atttatatgg agagaccgtt aaaaaatgca gaatatacca ttcacatcga agtgccgcca
     6781 aatcctttct gggcttccat tggtttatct gtatcaccgc ttccgttggg aagtggaatg
     6841 cagtatgaga gctcggtttc tcttggatac ttaaatcaat catttcaaaa tgcagttatg
     6901 gaagggatac gctatggttg cgaacaagga ttatatggtt ggaatgtgac ggattgtaaa
     6961 atctgtttta agtatggctt atactatagc cctgttagta ccccagcaga ttttcggatg
     7021 cttgctccta ttgtattgga acaagtctta aaaaaagctg gaacagaatt gttagagcca
     7081 tatcttagtt ttaaaattta tgcgccacag gaatatcttt cacgagcata caacgatgct
     7141 cctaaatatt gtgcgaacat cgtagacact caattgaaaa ataatgaggt cattcttagt
     7201 ggagaaatcc ctgctcggtg tattcaagaa tatcgtagtg atttaacttt ctttacaaat
     7261 ggacgtagtg tttgtttaac agagttaaaa gggtaccatg ttactaccgg tgaacctgtt
     7321 tgccagcccc gtcgtccaaa tagtcggata gataaagtac gatatatgtt caataaaata
     7381 acttagtgta ttttatgttg ttatataaat atggtttctt gttaaataag atgaaatatt
     7441 ttttaataaa gatttgaatt aaagtgtaaa ggaggaatta attatgaagt gtaatgaatg
     7501 taacagggtt aaatacaacc aaattttaat ttggctttgc gttttatctt tttttagcgt
     7561 attaaatgaa atggttttga acgtctcatt acctgatatt gcaaatgatt ttaataaacc
     7621 acctgcgagt acaaactggg tgaacacagc ctttatgtta accttttcca ttggaacagc
     7681 tgtatatgga aagctatctg atcaattagg catcaaaagg ttactcctat ttggaattat
     7741 aataaattgt ttcgggtcgg taattgggtt tgttggccat tctttctttt ccttacttat
     7801 tatggctcgt tttattcaag gggctggtgc agctgcattt ccagcactcg taatggttgt
     7861 agttgcgcgc tatattccaa aggaaaatag gggtaaagca tttggtctta ttggatcgat
     7921 agtagccatg ggagaaggag tcggtccagc gattggtgga atgatagccc attatattca
     7981 ttggtcctat cttctactca ttcctatgat aacaattatc actgttccgt ttcttatgaa
     8041 attattaaag aaagaagtaa ggataaaagg tcattttgat atcaaaggaa ttatactaat
     8101 gtctgtaggc attgtatttt ttatgttgtt tacaacatca tatagcattt cttttcttat
     8161 cgttagcgtg ctgtcattcc tgatatttgt aaaacatatc aggaaagtaa cagatccttt
     8221 tgttgatccc ggattaggga aaaatatacc ttttatgatt ggagttcttt gtgggggaat
     8281 tatatttgga acagtagcag ggtttgtctc tatggttcct tatatgatga aagatgttca
     8341 ccagctaagt actgccgaaa tcggaagtgt aattattttc cctggaacaa tgagtgtcat
     8401 tattttcggc tacattggtg ggatacttgt tgatagaaga ggtcctttat acgtgttaaa
     8461 catcggagtt acatttcttt ctgttagctt tttaactgct tcctttcttt tagaaacaac
     8521 atcatggttc atgacaatta taatcgtatt tgttttaggt gggctttcgt tcaccaaaac
     8581 agttatatca acaattgttt caagtagctt gaaacagcag gaagctggtg ctggaatgag
     8641 tttgcttaac tttaccagct ttttatcaga gggaacaggt attgcaattg taggtggttt
     8701 attatccata cccttacttg atcaaaggtt gttacctatg gaagttgatc agtcaactta
     8761 tctgtatagt aatttgttat tacttttttc aggaatcatt gtcattagtt ggctggttac
     8821 cttgaatgta tataaacatt ctcaaaggga tttctaaatc gttaagggat caactttggg
     8881 agagagttca aaattgatcc tttttttata acaggaattc aaatcttttt gttccattaa
     8941 agggcgcgat tgctgaataa aagatacgag agacctctct tgtatctttt ttattttgag
     9001 tggttttgtc cgttacacta gaaaaccgaa agacaataaa aattttattc ttgctgagtc
     9061 tggctttcgg taagctagac aaaacggaca aaataaaaat tggcaagggt ttaaaggtgg
     9121 agattttttg agtgatcttc tcaaaaaata ctacctgtcc cttgctgatt tttaaacgag
     9181 cacgagagca aaacccccct ttgctgaggt ggcagagggc aggttttttt gtttcttttt
     9241 tctcgtaaaa aaaagaaagg tcttaaaggt tttatggttt tggtcggcac tgccgacagc
     9301 ctcgcagagc acacacttta tgaatataaa gtatagtgtg ttatacttta cttggaagtg
     9361 gttgccggaa agagcgaaaa tgcctcacat ttgtgccacc taaaaaggag cgatttacat
     9421 atgagttatg cagtttgtag aatgcaaaaa gtgaaatcag ctggactaaa aggcatgcaa
     9481 tttcataatc aaagagagcg aaaaagtaga acgaatgatg atattgacca tgagcgaaca
     9541 cgtgaaaatt atgatttgaa aaatgataaa aatattgatt acaacgaacg tgtcaaagaa
     9601 attattgaat cacaaaaaac aggtacaaga aaaacgagga aagatgctgt tcttgtaaat
     9661 gagttgctag taacatctga ccgagatttt tttgagcaac tggatccagg agaacaaaaa
     9721 cgattttttg aggaaagtta taaattattt tccgaacgat atggcaagca aaatattgct
     9781 tatgcaacag ttcataatga tgagcaaacc cctcacatgc atttaggtgt tgtgcctatg
     9841 cgtgatggaa aactgcaagg aaaaaatgtg tttaatcgtc aagaactgtt atggctacaa
     9901 gataaattcc ccgagcatat gaaaaaacag ggttttgagt tgaagcgtgg tgaacgtggc
     9961 tctgaccgta aacatattga gacagctaaa tttaaaaaac aaactttgga aaaagagatt
    10021 gattttctag aaaaaaattt agcagttaaa aaagatgaat ggactgctta tagcgataaa
    10081 gttaaatcag atttagaagt accagcgaaa cgacacatga aaagtgttga agtgccaacg
    10141 ggtgaaaagt ccatgtttgg tttgggaaaa gaaataatga aaacagaaaa gaaaccaacc
    10201 aaaaatgttg ttatatcgga gcgtgattat aaaaacttag tgactgctgc gagagataac
    10261 gataggttaa aacagcatgt tagaaatctc atgagtactg atatggcgag agaatataaa
    10321 aaattaagta aagaacatgg gcaagttaaa gaaaaatata gtggtcttgt agagcgattt
    10381 aatgaaaatg taaatgatta taatgagttg cttgaagaaa acaagtcttt aaagtctaaa
    10441 ataagcgatt taaagcgtga tgtgagttta atctatgaaa gcactaagga attccttaag
    10501 gaacgtacag acggcttaaa agcctttaaa aacgttttta aggggtttgt agacaaggta
    10561 aaggataaaa cagcacaatt ccaagaaaaa cacgatttag aacctaaaaa gaacgaattt
    10621 gaactaactc ataaccgaga ggtaaaaaaa gaacgaagtc gagatcaggg aatgagttta
    10681 taaaataaaa aaagcacctg aaaaggtgtc tttttttgat ggttttgaac ttgttctttc
    10741 ttatcttgat atatatagaa ataacgtcat ttttatttta gttgctgaaa ggtgcgttga
    10801 agtgttggta tgtatgtgtt ttaaagtatt gaaaaccctt aaaattggtt gcacagaaaa
    10861 accccatctg ttaaagttat aagtgaccaa acaaataact aaatagatgg gggtttcttt
    10921 taatattatg tgtcctaata gtagcattta ttcagatgaa aaatcaaggg ttttagtgga
    10981 caagacaaaa agtggaaaag tgagaccatg gagagaaaag aaaatcgcta atgttgatta
    11041 ctttgaactt ctgcatattc ttgaatttaa aaaggctgaa agagtaaaag attgtgctga
    11101 aatattagag tataaacaaa atcgtgaaac aggcgaaaga aagttgtatc gagtgtggtt
    11161 ttgtaaatcc aggctttgtc caatgtgcaa ctggaggaga gcaatgaaac atggcattca
    11221 gtcacaaaag gttgttgctg aagttattaa acaaaagcca acagttcgtt ggttgtttct
    11281 cacattaaca gttaaaaatg tttatgatgg cgaagaatta aataagagtt tgtcagatat
    11341 ggctcaaaga tttcgccgaa tgatgcaata taaaaaaatt aataaaaatc ttgttggttt
    11401 tatgcgtgca acggaagtga caataaataa taaagataat tcttataatc agcacatgca
    11461 tgtattggta tgtgtggaac caacttattt taagaataca gaaaactacg tgaatcaaaa
    11521 acaatggatt caattttgga aaaaggcaat gaaattagac tatgatccaa atgtaaaagt
    11581 tcaaatgatt cgaccgaaaa ataaatataa atcggatata caatcggcaa ttgacgaaac
    11641 tgcaaaatat cctgtaaagg atacggattt tatgaccgat gatgaagaaa agaatttgaa
    11701 acgtttgtct gatttggagg aaggtttaca ccgtaaaagg ttaatctcct atggtggttt
    11761 gttaaaagaa atacataaaa aatggttctg ttgcaaagtt ttaaataaag aataaaatcc
    11821 c
//
LOCUS       DASHHB010000210         5745 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.106, whole genome shotgun sequence.
ACCESSION   DASHHB010000210 DASHHB010000000
VERSION     DASHHB010000210.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5745)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5745)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5745
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.106"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(20..235)
                     /locus_tag="U2K71_003035"
     CDS             complement(20..235)
                     /locus_tag="U2K71_003035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002343843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7317023.1"
                     /translation="MSKKGIIVNEWEKLNRISNRRLNAQTMCTPLKMQWSIDRITIVG
                     TLRENIYYHTPHDVLILDFEQGSVAKF"
     gene            complement(778..2928)
                     /locus_tag="U2K71_003036"
                     /pseudo
     CDS             complement(778..2928)
                     /locus_tag="U2K71_003036"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000684984.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Tn3 family transposase"
     gene            3074..3649
                     /locus_tag="U2K71_003037"
     CDS             3074..3649
                     /locus_tag="U2K71_003037"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001226076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7317024.1"
                     /translation="MRKIGYIRVSSTNQNPSRQFQQLNEIGMDIIYEEKVSGATKDRE
                     QLQKVLDDLQEDDIIYVTDLTRITRSTQDLFELIDNIRDKKASLKSLKDTWLDLSEDN
                     PYSQFLITVMAGVNQLERDLIRMRQREGIELAKKEGKFKGRLKKYHKNHAGMNYAVKL
                     YKEGNMTVNQICEITNVSRASLYRKLSEVNN"
     gene            3863..4558
                     /gene="vanR"
                     /locus_tag="U2K71_003038"
     CDS             3863..4558
                     /gene="vanR"
                     /locus_tag="U2K71_003038"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001280781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance DNA-binding
                     response regulator VanR"
                     /protein_id="HEM7317025.1"
                     /translation="MSDKILIVDDEHEIADLVELYLKNENYTVFKYYTAKEALECIDK
                     SEIDLAILDIMLPGTSGLTICQKIRDKHTYPIIMLTGKDTEVDKITGLTIGADDYITK
                     PFRPLELIARVKAQLRRYKKFSGVKEQNENVIVHSGLVINVNTHECYLNEKQLSLTPT
                     EFSILRILCENKGNVVSSELLFHEIWGDEYFSKSNNTITVHIRHLREKMNDTIDNPKY
                     IKTVWGVGYKIEK"
     gene            4536..5690
                     /locus_tag="U2K71_003039"
     CDS             4536..5690
                     /locus_tag="U2K71_003039"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002305818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VanA-type vancomycin resistance histidine kinase
                     VanS"
                     /protein_id="HEM7317026.1"
                     /translation="MVIKLKNKKNDYSKLERKLYMYIVAIVVVAIVFVLYIRSMIRGK
                     LGDWILSILENKYDLNHLDAMKLYQYSIRNNIDIFIYVAIVISILILCRVMLSKFAKY
                     FDEINTGIDVLIQNEDKQIELSAEMDVMEQKLNTLKRTLEKREQDAKLAEQRKNDVVM
                     YLAHDIKTPLTSIIGYLSLLDEAPDMPVDQKAKYVHITLDKAYRLEQLIDEFFEITRY
                     NLQTITLTKTHIDLYYMLVQMTDEFYPQLSAHGKQAVIHAPEDLTVSGDPDKLARVFN
                     NILKNAAAYSEDNSIIDITAGLSGDVVSIEFKNTGSIPKDKLAAIFEKFYRLDNARSS
                     DTGGAGLGLAIAKEIIVQHGGQIYAESNDNYTTFRVELPAMPDLVDKRRS"
BASE COUNT         1797 a         1104 c         1087 g         1757 t
ORIGIN      
        1 accttatttg atagtagatt taaaactttg caacagaacc ctgttcaaaa tctaaaatca
       61 acacatcatg gggagtgtga taataaatat tttcccttaa tgtaccaaca atggtaattc
      121 ggtcaattga ccattgcatt tttaaggggg tgcacatagt ttgtgcattt aaccgcctgt
      181 tagaaatacg gttaagtttt tcccactcat ttacgattat gcctttttta ctcaaaaata
      241 gctcctcctc aacgaaaaaa gagattcttg gaaagaattt ccagagttca cgtaactcta
      301 aaaatccaca tccaaaaatc tctagcttga agaaacctaa aaatattcgt ataatgaact
      361 tgagttacgg aactaggtga agtagtttcc gtaaaggtcg agagtgcgtc aacactgctc
      421 gactattttt ttactctttt ttctcatttt ctttgattat agcactcctc tagaaaaaat
      481 aaaaagacct cagggaattt cattccccga accccttgag cctcctcctg ccgaaataga
      541 atggcgctgc cagcatttat ttcgttcagc tatcggctac acaatctaac acgaccgttc
      601 cgctaccagt cgccttacag gctccatttt cgctcctcta acgtgtttca ccgattgtgc
      661 cgataactaa acgaaataaa cgctaaaacc gctcagaacg caaaatatgc cattttagag
      721 ctattgtaga aaatatagtt tgaaatgcaa ttttcttctt tcagcttttt atttcccact
      781 tgtaaatcgg tagatggtgg ctccttttcc agttccataa tgtggatttg catctgcatg
      841 tagtgatgaa acacctagct gcattctcat accatctgac gaagatgttg taccgtcgcc
      901 ccaatagaaa ggcaattgta atttatgatg aaagtttact aatatggctt gggctttatt
      961 catggcatct tcatacatgc gccattgaga tacattggct agttgcttat atgtaagtcc
     1021 gggtgtggct tcggccatct tgctcaagcc aatattcatt cccattccta aaagggcagc
     1081 catgataatg attgtttctt ccttatctgg ttttcgatta ttggaagcat gagtgaattg
     1141 ctcatgaaat cctgttatat gggccacatc catgagtaaa tcagttaatt ttattcttgg
     1201 tagcatctga taaaggcttg cactaaattt ttttgcttct tctggaacat ctttttctaa
     1261 gcgtgcaagt gatagctttc ctttttcaag agaaacccca tctaacttat tggaattggc
     1321 agctaaccac tttaaccttt cattaaagct gctggttctc tccgttatat aatcttcgaa
     1381 tgataaacta actgataatc tcgtattccc cttcgattga ttccatgtat cttccgaaaa
     1441 caaatattcc tcaaaatccc tatattgtct gctgccaaca atggaaacat ctcctgcccg
     1501 aacatgctcc cgaagttctg ttaaaacagc catttcatag taatgacgat taattgttgt
     1561 accatcatcc tcgtataaat gtcttttcca tcgttttgaa ataaaatcca caggtgagtc
     1621 atcaggcact tttcgctttc cagattcgtt cattcctcgg ataatctcaa cagcttgtaa
     1681 aagtggctca tttgcctttg tagaatgaaa ttccaatact cttaatagcg ttggcgtata
     1741 ttttcttagt gaataaaacc gtttttgcag taagtctaaa taatcatagt cggcaggacg
     1801 tgcaagttcc tgagcctctt ctactgaaga gacaaaggta ttccattcaa taaccgattc
     1861 taaaacctta aaaacgtcta atttttcctc tcttgcttta attaatgctt gtccgatgtt
     1921 cgtaaagtgt ataactttct catttagctt tttaccgttt tgtttctgga tttcctcttg
     1981 agccttacga ccttttgata acaaactaag tatttgccta tcatgaattt caaacgcttt
     2041 atccgttagc tcctgagtaa gttgtaataa atagatggtt aatatcgaat aacgtttatt
     2101 ttcttgaaag tcacggaatg catacggctc gtatcttgag cctaagcgag acagctgcaa
     2161 caggcggtta cggtgcaaat gactaatttg cactgtttct aaatccattc ctcgtatgta
     2221 ttcgagtcgt tctattattt ttagaaaagt ttcgggtgaa ggatgacccg gtggctcttt
     2281 taaccaaccc aatatcgttt tattggattc ggatggatgc tgcgaggtaa taatcccttc
     2341 aagcttttct ttttgctcat ttgttagaga tttactaacc gtattaaata gcttcttttc
     2401 agccattgcc cttgcttccc acaccattct ttcaagtgta gtgatagcag gcagtataat
     2461 tttgtttttt cttagaaaat ctatgcattc atgcagtaga tgaatggcat caccattttc
     2521 caaagctaat tgatgaaggt acttaaatgt cattcgatat tcactcaggg taaaagttac
     2581 aaagtcgtat tcacttcgaa tttctttcaa atgatcccaa agtgtatttt ccctttgagg
     2641 ataatgatca agcgaggatg gactaacacc aatctgtttc gatatatatt gtatgaccga
     2701 atctgggatg cttttgatat gagtgtatgg ccaaccggga taccgaagaa cagctaattg
     2761 aacagcaaat cctaaacggt tttcttccct ccttcgctta ttaactattt ctaaatcccg
     2821 tttggaaaaa gtgaagtagg tccccagtat ccattcatct tcagggattt gcataaaagc
     2881 ctgtctctgt tccggtgtaa gcaattctct acctctcgca attttcattc agtatcattc
     2941 catttctgta ttttcaattt attagttcaa ttatatatca atagagtgta ctctattgat
     3001 acaaatgtag tagactgata aaatcatagt taagagcgtc tcataagact tgtctcaaaa
     3061 atgaggtgat attttgcgga aaatcggtta tattcgtgtc agttcgacta accagaatcc
     3121 ttcaagacaa tttcagcagt tgaacgagat cggaatggat attatatatg aagagaaagt
     3181 ttcaggagca acaaaggatc gcgagcaact tcaaaaagtg ttagacgatt tacaggaaga
     3241 tgacatcatt tatgttacag acttaactcg aatcactcgt agtacacaag atctatttga
     3301 attaatcgat aacatacgag ataaaaaggc aagtttaaaa tcactaaaag atacatggct
     3361 tgatttatca gaagataatc catacagcca attcttaatt actgtaatgg ctggtgttaa
     3421 ccaattagag cgagatctta ttcggatgag acaacgtgaa gggattgaat tggctaagaa
     3481 agaaggaaag tttaaaggtc gattaaagaa gtatcataaa aatcacgcag gaatgaatta
     3541 tgcggtaaag ctatataaag aaggaaatat gactgtaaat caaatttgtg aaattactaa
     3601 tgtatctagg gcttcattat acaggaaatt atcagaagtg aataattagc cattctgtat
     3661 tccgctaatg ggcaatattt ttaaagaaga aaaggaaact ataaaatatt aacagcctcc
     3721 tagcgatgcc gaaaagccct ttgataaaaa aagaatcatc atcttaagaa attcttagtc
     3781 atttattatg taaatgctta taaattcggc cctataatct gataaattat taagggcaaa
     3841 cttatgtgaa agggtgataa ctatgagcga taaaatactt attgtggatg atgaacatga
     3901 aattgccgat ttggttgaat tatacttaaa aaacgagaat tatacggttt tcaaatacta
     3961 taccgccaaa gaagcattgg aatgtataga caagtctgag attgaccttg ccatattgga
     4021 catcatgctt cccggcacaa gcggccttac tatctgtcaa aaaataaggg acaagcacac
     4081 ctatccgatt atcatgctga ccgggaaaga tacagaggta gataaaatta cagggttaac
     4141 aatcggcgcg gatgattata taacgaagcc ctttcgccca ctggagttaa ttgctcgggt
     4201 aaaggcccag ttgcgccgat acaaaaaatt cagtggagta aaggagcaga acgaaaatgt
     4261 tatcgtccac tccggccttg tcattaatgt taacacccat gagtgttatc tgaacgagaa
     4321 gcagttatcc cttactccca ccgagttttc aatactgcga atcctctgtg aaaacaaggg
     4381 gaatgtggtt agctccgagc tgctatttca tgagatatgg ggcgacgaat atttcagcaa
     4441 gagcaacaac accatcaccg tgcatatccg gcatttgcgc gaaaaaatga acgacaccat
     4501 tgataatccg aaatatataa aaacggtatg gggggttggt tataaaattg aaaaataaaa
     4561 aaaacgacta ttccaaacta gaacgaaaac tttacatgta tatcgttgca attgttgtgg
     4621 tagcaattgt attcgtgttg tatattcgtt caatgatccg agggaaactt ggggattgga
     4681 tcttaagtat tttggaaaac aaatatgact taaatcacct ggacgcgatg aaattatatc
     4741 aatattccat acggaacaat atagatatct ttatttatgt ggcgattgtc attagtattc
     4801 ttattctatg tcgcgtcatg ctttcaaaat tcgcaaaata ctttgacgag ataaataccg
     4861 gcattgatgt acttattcag aacgaagata aacaaattga gctttctgcg gaaatggatg
     4921 ttatggaaca aaagctcaac acattaaaac ggactctgga aaagcgagag caggatgcaa
     4981 agctggccga acaaagaaaa aatgacgttg ttatgtactt ggcgcacgat attaaaacgc
     5041 cccttacatc cattatcggt tatttgagcc tgcttgacga ggctccagac atgccggtag
     5101 atcaaaaggc aaagtatgtg catatcacgt tggacaaagc gtatcgactc gaacagctaa
     5161 tcgacgagtt ttttgagatt acacggtata acctacaaac gataacgcta acaaaaacgc
     5221 acatagacct atactatatg ctggtgcaga tgaccgatga attttatcct cagctttccg
     5281 cacatggaaa acaggcggtt attcacgccc ccgaggatct gaccgtgtcc ggcgaccctg
     5341 ataaactcgc gagagtcttt aacaacattt tgaaaaacgc cgctgcatac agtgaggata
     5401 acagcatcat tgacattacc gcgggcctct ccggggatgt ggtgtcaatc gaattcaaga
     5461 acactggaag catcccaaaa gataagctag ctgccatatt tgaaaagttc tataggctgg
     5521 acaatgctcg ttcttccgat acgggtggcg cgggacttgg attggcgatt gcaaaagaaa
     5581 ttattgttca gcatggaggg cagatttacg cggaaagcaa tgataactat acgacgttta
     5641 gggtagagct tccagcgatg ccagacttgg ttgataaaag gaggtcctaa gagatgtata
     5701 taattttggc tctttgtcaa atgatgttgg taaagagaaa tctta
//
LOCUS       DASHHB010000211         5287 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.110, whole genome shotgun sequence.
ACCESSION   DASHHB010000211 DASHHB010000000
VERSION     DASHHB010000211.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 5287)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5287)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5287
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.110"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            <1..188
                     /locus_tag="U2K71_003040"
     CDS             <1..188
                     /locus_tag="U2K71_003040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018579902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7317027.1"
                     /translation="MNISVSELARRIGQTPQNFNKKLQRETVTLDELKAIADVLGVKF
                     VQAFILPDGEEIKISNE"
     gene            203..1072
                     /locus_tag="U2K71_003041"
     CDS             203..1072
                     /locus_tag="U2K71_003041"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002328890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HEM7317028.1"
                     /translation="MVGNVIQIVTEKLSSLPFIEGIVLGGSRARGTHTENSDIDIGIY
                     YNSDSFDLTAINQIATELDDENRNNLVVPPGAWGDWVNGGGWLVINGCHVDLILRDIK
                     RVEQIIKDTEQGIVTANYQTGHPHGYISAMYRGELAISKILYAKNESLCELKKQAEIY
                     PTALKKSLMNFFIFEAEFSLMFVKANAGAEDKYYIAGHVFRIISCLNQVLFACNNAYC
                     INEKKAIKLLETFEYKPEKYAERVNHIFEVLGLSLFECYDMTEKLYKEVNEIVSEINN
                     FLNEESSDERKQI"
     gene            1053..1787
                     /locus_tag="U2K71_003042"
     CDS             1053..1787
                     /locus_tag="U2K71_003042"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016250180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HEM7317029.1"
                     /translation="MKENKYDDNIFFQKYSQMSRSQKGLAGAGEWETLKKMLPDFKGK
                     RVLDLGCGYGWHCIYAMENGASSVVGVDISHKMLEVAKGKTHFPQIEYECCAIEDVDF
                     PEESFDVILSSLAFHYVADYENLIKKIYRMLKAGGNLVFTVEHPVFTAHGTQDWYYNE
                     KGEILHFPVDNYYYEGKRTAMFLEEKVTKYHRTLTTYLNTLLSNSFIINQIVEPQPPE
                     NMMDIPGMADEMRRPMMLIVSAKKKM"
     gene            1820..2728
                     /locus_tag="U2K71_003043"
     CDS             1820..2728
                     /locus_tag="U2K71_003043"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside nucleotidyltransferase ANT(6)-Ia"
                     /protein_id="HEM7317030.1"
                     /translation="MRSEKEMMDLVLSLAEQDERIRIVTLEGSRANINIPKDEFQDYD
                     ITYFVSDIEPFISNDDWLNQFGNIIMMQKPEDMELFPPEEKGFSYLMLFDDYNKIDLT
                     LLPLEELDNYLKGDKLIKVLIDKDCRIKRDIVPTDIDYHVRKPSAREYDDCCNEFWNV
                     TPYVIKGLCRKEILFAIDHFNQIVRHELLRMISWKVGIETGFKLSVGKNYKFIERYIS
                     EDLWEKLLSTYRMDSYENIWEALFLCHQLFRAVSGEVAERLHYAYPEYDRNITKYTRD
                     MYKKYTGKTGCLDSTYAADIEERREQ"
     gene            2725..3267
                     /gene="sat4"
                     /locus_tag="U2K71_003044"
     CDS             2725..3267
                     /gene="sat4"
                     /locus_tag="U2K71_003044"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000627290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="streptothricin N-acetyltransferase Sat4"
                     /protein_id="HEM7317031.1"
                     /translation="MITEMKAGHLKDIDKPSEPFEVIGKIIPRYENENWTFTELLYEA
                     PYLKSYQDEEDEEDEEADCLEYIDNTDKIIYLYYQDDKCVGKVKLRKNWNRYAYIEDI
                     AVCKDFRGQGIGSALINISIEWAKHKNLHGLMLETQDNNLIACKFYHNCGFKIGSVDT
                     MLYANFENNFEKAVFWYLRF"
     gene            3360..4154
                     /locus_tag="U2K71_003045"
                     /note="aph(3')-IIIa"
     CDS             3360..4154
                     /locus_tag="U2K71_003045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_032491780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase
                     APH(3')-IIIa"
                     /protein_id="HEM7317032.1"
                     /translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLK
                     MTDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEY
                     EDEQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWE
                     EDTPFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKW
                     YDIAFCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
     gene            4430..5002
                     /locus_tag="U2K71_003046"
     CDS             4430..5002
                     /locus_tag="U2K71_003046"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HTH domain-containing protein"
                     /protein_id="HEM7317033.1"
                     /translation="MNRINRVTSILIQLQSKKIIPAKEIAQRFNISLRTVYRDIRTLE
                     EAGIPIGSEAGKGYFLVEGFLLPPVMFTAAEVGALITAGKFLNCHGDESFIKDFDSAM
                     YKIKSILKHGEKNYAQELENSINVYSTSGQKNTLADNVIAAIQTAICNKRVISIQYPA
                     SGGQEPESRMIEPISLGFYEQNWYLIGFAG"
BASE COUNT         1821 a          795 c         1186 g         1485 t
ORIGIN      
        1 aaatgaatat aagtgtttcc gaacttgcta gacgtattgg ccagactcca cagaatttca
       61 ataaaaaatt acaacgagaa acagtaacct tggatgagtt gaaggccatt gctgatgtgc
      121 taggtgtcaa gtttgtgcag gcatttattt taccagatgg agaagaaata aaaatatcta
      181 acgaataaag gaggatgttt atatggtagg taatgttatt caaatagtaa cagagaaatt
      241 atcatctttg ccttttatag aaggcatcgt attagggggc tcacgtgcaa gaggcaccca
      301 tacagaaaat tctgatatag atatcggaat ctattacaat tcagactcat ttgacctgac
      361 agcgattaat caaattgcta cagagttgga tgatgagaat agaaacaacc ttgttgtacc
      421 tcccggagcg tggggtgatt gggttaatgg cggtggatgg ttagttataa acgggtgtca
      481 tgttgacttg atattacgtg atataaaacg ggtggaacaa ataatcaaag atacggagca
      541 aggaattgtt actgccaatt atcagaccgg gcatccccat ggttatataa gtgctatgta
      601 tcgtggagaa ttagcgatta gtaaaatact atatgctaag aacgaaagct tatgcgaatt
      661 aaaaaagcag gcagaaattt accctactgc tctaaagaaa agcttgatga acttttttat
      721 atttgaagct gaattctctt taatgtttgt aaaagcaaat gcgggggcag aggataaata
      781 ttatattgca ggccatgttt ttcgtattat ttcatgctta aatcaagtac tatttgcatg
      841 taataatgct tattgcatta acgaaaagaa agctataaaa ctgcttgaaa cttttgaata
      901 taaacctgaa aaatatgccg aaagagttaa tcatattttt gaagtactcg gtctttctct
      961 ttttgaatgc tacgacatga ccgagaaact ttataaagaa gtgaatgaaa ttgtatcgga
     1021 gataaataac tttttaaacg aggagagttc agatgaaaga aaacaaatat gatgataata
     1081 tattttttca aaaatacagt caaatgagtc gctcgcagaa aggactggct ggtgcgggag
     1141 aatgggagac tttgaaaaag atgctacctg attttaaggg taagcgtgtg cttgatttag
     1201 gatgcggcta tggatggcac tgtatatatg cgatggaaaa cggtgcttcc tctgtagtag
     1261 gtgttgatat ttctcataaa atgctcgaag tagcaaaagg aaaaacccat tttccacaga
     1321 ttgaatatga atgctgtgcc atagaagatg tggatttccc agaggagagc tttgatgtaa
     1381 tactaagttc gcttgcgttt cattatgtag cagactatga gaatttaata aaaaagatat
     1441 ataggatgct gaaggctggt ggcaatttag tttttacagt tgaacatcct gtttttactg
     1501 ctcatggaac acaagactgg tattataacg aaaaaggaga aatactgcat ttcccggtgg
     1561 acaattatta ttatgagggc aaacggacag ctatgttttt ggaagaaaag gttacaaaat
     1621 atcatagaac actgaccaca tatctaaata cactgctttc aaatagtttt ataataaatc
     1681 agattgtgga gccacagccg ccagagaaca tgatggatat tccggggatg gcggatgaaa
     1741 tgcgacgccc aatgatgctg attgtatcgg caaaaaagaa gatgtaataa tatagaaaaa
     1801 ataaacgagg agtatgtaaa tgagatcaga aaaagaaatg atggatttag tactttcttt
     1861 agcagaacag gatgaacgta ttcgaattgt gacccttgag gggtcacgcg caaatattaa
     1921 tatacctaaa gatgaatttc aggattatga tattacatat tttgtaagtg atatagaacc
     1981 gtttatatct aatgatgact ggcttaatca atttgggaat ataataatga tgcaaaagcc
     2041 ggaggatatg gaattattcc cacctgaaga aaagggattt tcctatctta tgctatttga
     2101 tgattacaat aaaattgatc ttaccttatt gcccttggaa gagttagata attacctaaa
     2161 gggcgataaa ttaataaagg ttctaattga taaagattgt agaattaaaa gggacatagt
     2221 tccgactgat atagattatc atgtaagaaa gccaagcgca agggagtatg atgattgctg
     2281 caatgaattt tggaatgtaa caccttatgt tattaaagga ttgtgccgta aggaaatttt
     2341 atttgctatt gatcatttta atcagattgt tcgccatgag ctgctgagaa tgatatcatg
     2401 gaaggtcggc atcgaaacag gctttaaatt aagtgtaggc aagaactata agtttattga
     2461 aaggtatata tccgaggatt tgtgggagaa acttttgtcc acctaccgga tggattccta
     2521 tgaaaacata tgggaagcat tatttctatg ccatcaattg ttcagggcgg tatccggtga
     2581 ggtggcggaa aggcttcatt atgcctatcc ggagtatgat aggaatataa caaaatatac
     2641 cagggacatg tataaaaaat acactggtaa aaccggctgc ctggatagca catatgccgc
     2701 tgatatagaa gagaggcggg aacagtgatt acagaaatga aagcagggca cctgaaagat
     2761 atcgataaac ccagcgaacc atttgaggtg ataggtaaga ttataccgag gtatgaaaac
     2821 gagaattgga cctttacaga attactctat gaagcgccat atttaaaaag ctaccaagac
     2881 gaagaggatg aagaggatga ggaggcagat tgccttgaat atattgacaa tactgataag
     2941 ataatatatc tttactacca agacgataaa tgcgtcggaa aagttaaact gcgaaaaaat
     3001 tggaaccggt acgcttatat agaagatatc gccgtatgta aggatttcag ggggcaaggc
     3061 ataggcagcg cgcttatcaa tatatctata gaatgggcaa agcataaaaa cttgcatgga
     3121 ctaatgcttg aaacccagga caataacctt atagcttgta aattctatca taattgtggt
     3181 ttcaaaatcg gctccgtcga tactatgtta tacgccaact ttgaaaacaa ctttgaaaaa
     3241 gctgttttct ggtatttaag gttttagaat gcaaggaaca gtgaattgga gttcgtcttg
     3301 ttataattag cttcttgggg tatctttaaa tactgtagaa aagaggaagg aaataataaa
     3361 tggctaaaat gagaatatca ccggaattga aaaaactgat cgaaaaatac cgctgcgtaa
     3421 aagatacgga aggaatgtct cctgctaagg tatataagct ggtgggagaa aatgaaaacc
     3481 tatatttaaa aatgacggac agccggtata aagggaccac ctatgatgtg gaacgggaaa
     3541 aggacatgat gctatggctg gaaggaaagc tgcctgttcc aaaggtcctg cactttgaac
     3601 ggcatgatgg ctggagcaat ctgctcatga gtgaggccga tggcgtcctt tgctcggaag
     3661 agtatgaaga tgaacaaagc cctgaaaaga ttatcgagct gtatgcggag tgcatcaggc
     3721 tctttcactc catcgacata tcggattgtc cctatacgaa tagcttagac agccgcttag
     3781 ccgaattgga ttacttactg aataacgatc tggccgatgt ggattgcgaa aactgggaag
     3841 aagacactcc atttaaagat ccgcgcgagc tgtatgattt tttaaagacg gaaaagcccg
     3901 aagaggaact tgtcttttcc cacggcgacc tgggagacag caacatcttt gtgaaagatg
     3961 gcaaagtaag tggctttatt gatcttggga gaagcggcag ggcggacaag tggtatgaca
     4021 ttgccttctg cgtccggtcg atcagggagg atatcgggga agaacagtat gtcgagctat
     4081 tttttgactt actggggatc aagcctgatt gggagaaaat aaaatattat attttactgg
     4141 atgaattgtt ttagtaccta gatttagatg tctaaaaagc tttaactaca agctttttag
     4201 acatctaatc ttttctgaag tacatccgca actgtccata ctctgatgtt ttatatcttt
     4261 tctaaaagtt cgctagatag agttctatat tagagatgta aagagttttg gtgaagaggt
     4321 ttgtgagtat ggcttttata accagggtgc aatgagaagc actaacagca gctagcttaa
     4381 gaacgcatag gatatacttt ttatggaagt cacagaggag gaataaaatt tgaacaggat
     4441 taacagagta acctctattc ttattcagct gcaatcaaaa aaaataattc ctgccaaaga
     4501 aattgcacag cgatttaata taagcttaag gacagtttac agagatatcc ggacacttga
     4561 agaagccgga ataccaattg gatctgaggc cggaaaagga tattttcttg tagagggctt
     4621 cctacttccg ccggtaatgt ttacagcggc ggaagtgggt gcattgatta cagcagggaa
     4681 atttttaaat tgccatggag atgaatcatt tataaaggat tttgattcag ctatgtataa
     4741 aatcaaatct attttgaagc atggtgaaaa aaactatgca caggagttgg agaacagtat
     4801 taacgtgtac agcacatctg gacagaaaaa tacattggcg gataacgtta ttgcagcaat
     4861 tcagactgca atctgcaata aaagggtaat atcaattcaa tatcctgcat caggagggca
     4921 agaaccggaa agcagaatga tagagcctat atcactgggg ttttatgaac agaactggta
     4981 cttaatcggt tttgcaggct aagaaatgaa tataggaatt ttcgtgtgga tcggattaaa
     5041 agcatttgca tagaagaaga gctctgtcaa agccatgatg gctctttgga acaaatatta
     5101 aaacaaatgc tttcatataa aaaactatat gccctacaat gccgttgtaa aaattgggga
     5161 aaagggatat attgatttta tatctttctc aaaataaata taaaagccct ttaaagaggg
     5221 cttttatata ttaatcacaa atcacttatc acaaatcaca agtgattaat cacaaatcac
     5281 ttgtgat
//
LOCUS       DASHHB010000212         4866 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.115, whole genome shotgun sequence.
ACCESSION   DASHHB010000212 DASHHB010000000
VERSION     DASHHB010000212.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4866)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4866)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4866
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.115"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            365..1276
                     /locus_tag="U2K71_003047"
     CDS             365..1276
                     /locus_tag="U2K71_003047"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001812592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala carboxypeptidase VanY-A"
                     /protein_id="HEM7317034.1"
                     /translation="MKKLFFLLLLLFLIYLGYDYVNEALFSQEKVEFQNYDQNPKEHL
                     ENSGTSENTQEKTITEEQVYQGNLLLINSKYPVRQESVKSDIVNLSKHDELINGYGLL
                     DSNIYMSKEIAQKFSEMVNDAVKGGVSHFIINSGYRDFDEQSVLYQEMGAEYALPAGY
                     SEHNSGLSLDVGSSLTKMERAPEGKWIEENAWKYGFILRYPEDKTELTGIQYEPWHIR
                     YVGLPHSAIMKEKNFVLEEYMDYLKEEKTISVSVNGEKYEIFYYPVTKNTTIHVPTNL
                     RYEISGNNIDGVIVTVFPGSTHTNSRR"
     gene            1429..1914
                     /gene="vanZ"
                     /locus_tag="U2K71_003048"
     CDS             1429..1914
                     /gene="vanZ"
                     /locus_tag="U2K71_003048"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000516404.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycopeptide resistance protein VanZ-A"
                     /protein_id="HEM7317035.1"
                     /translation="MGKILSRGLLALYLVTLIWLVLFKLQYNILSVFNYHQRSLNLTP
                     FTATGNFREMIDNVIIFIPFGLLLNVNFKEIGFLPKFAFVLVLSLTFEIIQFIFAIGA
                     TDITDVITNTVGGFLGLKLYGLSNKHMNQKKLDRVIIFVGILLLVLLLVYRTHLRINY
                     V"
     gene            complement(2201..2530)
                     /locus_tag="U2K71_003049"
     CDS             complement(2201..2530)
                     /locus_tag="U2K71_003049"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000754864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HEM7317036.1"
                     /translation="MKKVCQVTVVHKEKVNGIKNKLDQKDFSNLLVLGKCFSDFSRIK
                     IFYALETYKEMCVCDLAEILTASVATTSHHLRFLKKHGMAKSRQDGKVVYYSLANEDI
                     LSAIRVF"
     gene            2835..3164
                     /locus_tag="U2K71_003050"
     CDS             2835..3164
                     /locus_tag="U2K71_003050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005877489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB family transcriptional regulator"
                     /protein_id="HEM7317037.1"
                     /translation="MITKTRKQGNSIMLTVPKEFDVPNGVEVEAKLVENGILYEFVEP
                     KKEFFDFSEDILADILSEGYSKQEILKEFRNRKNALTSAFKSIAEDTVANSKPMTKEE
                     LAAEIGL"
     gene            3154..3441
                     /locus_tag="U2K71_003051"
     CDS             3154..3441
                     /locus_tag="U2K71_003051"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000312842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HEM7317038.1"
                     /translation="MAYEILPSKHVIKYLKKLKEKTLKEQFLTIIYDEIAVRPHSGEQ
                     KTGDLSGIWAIGFKYAGTTYRVAYEIKDNAVIPILLCGTHENFYEQLKKIR"
     gene            complement(3720..4283)
                     /locus_tag="U2K71_003052"
     CDS             complement(3720..4283)
                     /locus_tag="U2K71_003052"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000017403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HEM7317039.1"
                     /translation="MSGYIYGYARVSTRSQELNRQLDLLNEQNCNEILTEKMTGTKAV
                     RPQLNRLKDKIRPGDTVIVESFSRLGRSTKDLIDLVTYFEEHHVKLVSLKENFDTSTP
                     HGRLMMTVFQAFSQFERDLIVERTKEGLKSARARGRKGGRPRVNQKDVDRAVKLYKSQ
                     VYSVKEITEMTGISKATLYRYLKDNRE"
BASE COUNT         1705 a          735 c          837 g         1589 t
ORIGIN      
        1 cttatttgat agtagattta aaactttgca acagaaccat tgaagatgta aggcagagcg
       61 atattgcggt cattatctgc gtgcgctgcg gcaagatagc ctgataataa gactgatcgc
      121 atagaggggt ggtatttcac accgcccatt gtcaacaggc agttcagcct cgttaaattc
      181 agcatgggta tcacttatga aaattcatct acattggtga taatagtaaa tccagtaggg
      241 cgaaataatt gactgtaatt tacggggcaa aacggcacaa tctcaaacga gattgtgccg
      301 tttaagggga agattctaga aatatttcat acttccaact atatagttaa ggaggagact
      361 gaaaatgaag aagttgtttt ttttattgtt attgttattc ttaatatact taggttatga
      421 ctacgttaat gaagcactgt tttctcagga aaaagtcgaa tttcaaaatt atgatcaaaa
      481 tcccaaagaa catttagaaa atagtgggac ttctgaaaat acccaagaga aaacaattac
      541 agaagaacag gtttatcaag gaaatctgct attaatcaat agtaaatatc ctgttcgcca
      601 agaaagtgtg aagtcagata tcgtgaattt atctaaacat gacgaattaa taaatggata
      661 cgggttgctt gatagtaata tttatatgtc aaaagaaata gcacaaaaat tttcagagat
      721 ggtcaatgat gctgtaaagg gtggcgttag tcattttatt attaatagtg gctatcgaga
      781 ctttgatgag caaagtgtgc tttaccaaga aatgggggct gagtatgcct taccagcagg
      841 ttatagtgag cataattcag gtttatcact agatgtagga tcaagcttga cgaaaatgga
      901 acgagcccct gaaggaaagt ggatagaaga aaatgcttgg aaatacgggt tcattttacg
      961 ttatccagag gacaaaacag agttaacagg aattcaatat gaaccatggc atattcgcta
     1021 tgttggttta ccacatagtg cgattatgaa agaaaagaat ttcgttctcg aggaatatat
     1081 ggattaccta aaagaagaaa aaaccatttc tgttagtgta aatggggaaa aatatgagat
     1141 cttttattat cctgttacta aaaataccac cattcatgtg ccgactaatc ttcgttatga
     1201 gatatcagga aacaatatag acggtgtaat tgtgacagtg tttcccggat caacacatac
     1261 taattcaagg aggtaaggat ggcggaatga aaccaacgaa attaatgaac agcattattg
     1321 tactagcact tttggggtaa cgttagcttt ttaatttaaa acccacgtta actaggacat
     1381 tgctatacta atgatacaac ttaaacaaaa gaattagagg aaattatatt gggaaaaata
     1441 ttatctagag gattgctagc tttatattta gtgacactaa tctggttagt gttattcaaa
     1501 ttacaataca atattttatc agtatttaat tatcatcaaa gaagtcttaa cttgactcca
     1561 tttactgcta ctgggaattt cagagagatg atagataatg ttataatctt tattccattt
     1621 ggcttgcttt tgaatgtcaa ttttaaagaa atcggatttt tacctaagtt tgcttttgta
     1681 ctggttttaa gtcttacttt tgaaataatt caatttatct tcgctattgg agcgacagac
     1741 ataacagatg taattacaaa tactgttgga ggctttcttg gactgaaatt atatggttta
     1801 agcaataagc atatgaatca aaaaaaatta gacagagtta ttatttttgt aggtatactt
     1861 ttgctcgtat tattgctcgt ttaccgtacc catttaagaa taaattacgt gtaagatgtc
     1921 taaatcaagc aatctgatct ttcatacaca taaagatatt gaatgaattg gattagatgg
     1981 aaaacgggat gtggggaaac tcgcccgtag gtgtgaagtg aggggaaaac cggtgataaa
     2041 gtaaaaagct tacctaacac tatagtaaca aagaaagccc aattatcaat tttagtgctg
     2101 aggaattggt ctctttaata aatttcctta acgttgtaaa tccgcatttt cctgacggta
     2161 cccccaaact aagatttcat agatagattt tctgataact ttaaaaaaca cgaatagcgg
     2221 aaaggatgtc ttcattcgct aaagagtaat aaactacttt tccatcttga cgtgattttg
     2281 ccattccatg tttttttaaa aaacgtaaat ggtgtgacgt tgtagcgaca ctagcagtaa
     2341 gtatttctgc taaatcacag acgcacattt ccttataggt ttctaaagca tagaaaattt
     2401 taattctaga aaaatcgctg aaacatttcc ctaacacaag taagtttgaa aaatcttttt
     2461 gatccaattt attttttatt ccatttactt tttctttatg tacgacagtc acttggcaaa
     2521 cttttttcat tcatctcacc tcacattcaa acgattattt gaatgaagta tacctatttg
     2581 atcactcttt tgtcaatcta ccttaattcc aatacactat tataagcaat ttcatgtatt
     2641 gaagtaaact tacttgcaag tgcgtttttg attccaagtt aaaaacatat tattattcta
     2701 cataatttcc taatcaaata aaactccttt gaaaattgtt ctaaaggagt tttattattt
     2761 tgtatttcta ctaaaaatag cgtacaatgt aattactaag taattacgaa atgtaaaata
     2821 atggaggaaa taatatgatt acaaaaacaa gaaaacaagg aaattctatt atgttaacgg
     2881 tccctaaaga attcgatgtg cccaatggtg ttgaagtaga agctaagctt gtagagaatg
     2941 gtatccttta tgagtttgta gagcctaaaa aagaattttt tgattttagt gaagatattt
     3001 tggctgatat tctttcagaa ggttatagca aacaagagat cttaaaagaa tttagaaata
     3061 gaaaaaatgc gttaacttct gcatttaaat ctattgctga agatactgtt gcaaattcta
     3121 aaccgatgac aaaagaggag ttggcagcag aaattggctt atgagattct accttctaaa
     3181 cacgtaataa agtatctaaa aaaattaaaa gagaaaacat taaaagaaca atttttaacg
     3241 ataatttatg atgagatagc agttcgtcct cattcgggag aacaaaaaac gggtgattta
     3301 tcaggaattt gggctattgg attcaagtat gcaggtacga catatcgcgt tgcatatgaa
     3361 attaaagata atgcagtgat acctatctta ctatgtggaa cgcatgaaaa tttttatgaa
     3421 caattaaaaa agattaggta agaacccaat gtttcaactt gagaggatag acgacacttt
     3481 aaaagataat ttttaacagc tgagagttgt aataccctat aattttaaac cactaaccta
     3541 tatatttatg aaaaatagaa aaaattaaaa cgcattttaa gcctctttca aattaaaagc
     3601 ttcatcctta tgcacaaaga gggcttaaaa tgcgttattt tatattgttt tttagtaata
     3661 ataaaaaaat cactctaatt ctcccataaa tattatagat tcctaaagat taaaatagat
     3721 tattccctat tgtcttttaa gtagcggtag agtgttgcct tgctaatacc agtcatttcc
     3781 gtaatttctt ttacgctata aacctgactt ttatacaatt ttactgctcg atcaacatct
     3841 ttctggttca ctcgtggacg gccaccttta cgacctctcg cacgtgcact tttcaaacct
     3901 tctttggtac gttcaacaat taaatcacgt tcgaactgac taaatgcttg gaagacagtc
     3961 atcattaagc gtccgtgagg cgtgctggta tcgaagtttt ctttcaagct caccagctta
     4021 acatggtgtt cttcaaaata ggtaacaagg tcaatcaaat cctttgtact acgacccaat
     4081 cgagaaaaac tttctacaat taccgtatct cccggacgaa ttttatcttt taaacgattt
     4141 agttggggac gaaccgcttt ggttcccgtc attttctcag ttaggatttc attacaattt
     4201 tgttcattga gtaagtctaa ttgacgattt agttcttgtg aacgtgtact cactcgggca
     4261 taaccataaa tgtatccact catgcttttc ttcctttcgt actttaattc tggcttgatt
     4321 gtatcataaa agggagttat agatacatag ttaatgcaac aagttatgat acaaaatatg
     4381 acctctttta tttaataaaa gaggtcattt cattttgtct cataatcgat tgtttttgat
     4441 acgagtgtgt atacgaaata aacaaaaaaa tcttaagtaa atttctagat acctaattta
     4501 atcctaacta gtgtatccta aagttaataa cgagtttatc acaatgaaaa ttataaaatg
     4561 ctcccatatt agatataaca tgcaatattt gaattagaac tacaaaattt attcgtttta
     4621 taaagtaaat aatgttgaga tactaaggaa tagaatatta taaggaattg agctgaatgt
     4681 attggataaa aaaagaataa ctttattaga gatggactat aaaaatgctc gtaattttct
     4741 tctgaagtct gaaagctatt tgaatgctca gctaccagaa tatattagtt ttgacgaaac
     4801 tttaaaatct gctaaaaaag ttttaacttc gccgtttgta taattaagcg agacaaataa
     4861 aaaagc
//
LOCUS       DASHHB010000213         4048 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.129, whole genome shotgun sequence.
ACCESSION   DASHHB010000213 DASHHB010000000
VERSION     DASHHB010000213.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 4048)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4048)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4048
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.129"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            322..1014
                     /locus_tag="U2K71_003053"
     CDS             322..1014
                     /locus_tag="U2K71_003053"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002318394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HEM7317040.1"
                     /translation="MKKKFITGGIVVAFLCLIGYTGTRYANEIILWGGETDIDTINEN
                     LTQLDHTLTDKEQAITDLTARLAQKEQTLQDSKADVQEYQTKVNELENQKNQSAAEKQ
                     NLESQLVSKDAQLQSKQNEINVKLDEINQKNDEINHLNADWTARLQEAQAKLSELQNT
                     VNGLNQQIATLTQEKTNVETQLTDTQKKLDEATAENSSLKQYIQKLEKAKDEVEDTAD
                     KSQQIVEEHLRK"
     gene            1341..1838
                     /gene="dfrG"
                     /locus_tag="U2K71_003054"
     CDS             1341..1838
                     /gene="dfrG"
                     /locus_tag="U2K71_003054"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000868795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethoprim-resistant dihydrofolate reductase
                     DfrG"
                     /protein_id="HEM7317041.1"
                     /translation="MKVSLIAAMDKNRVIGKENDIPWRIPKDWEYVKNTTKGHPIILG
                     RKNLESIGRALPDRRNIILTRDKGFTFNGCEIVHSIEDVFELCKNEEEIFIFGGEQIY
                     NLFFPYVEKMYITKIHHEFEGDTFFPEVNYEEWNEVFAQKGIKNDKNPYNYYFHVYER
                     KNLLS"
     gene            complement(1910..3862)
                     /locus_tag="U2K71_003055"
     CDS             complement(1910..3862)
                     /locus_tag="U2K71_003055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017431446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insertion element protein"
                     /protein_id="HEM7317042.1"
                     /translation="MTKLKRLATKEDEIVDVKIPISNEELKDRAKQYDLLTPKRFATR
                     YNKMLFLPTSFKWNGSEYPIQYNYCINPFCCNFGKEQHKFKDVKGKPSRYKMTGSSKD
                     KGHKGMYCNDNPIGRGVSQNCTVTPLSNWSVVEEIKRLIEINSIQDVEPDYQFHKEGC
                     SEEESTPFNEPKQFYKRGKSRGKSQRYQCKACKKFTNVLPKREETTTYHQQKNTILPM
                     FAKMVVGRVSVSRTCDILGIGVGTYYHKLEWLYRRCLEFLERYETQPLQTKKFNEMWL
                     NTDKMHYYLNNVRKKGQGSKKYTGFEDLNMQTYIVVSAEVLSRYVFRSDVAYDWNISM
                     DELNEDTRKFKEDHLNTFSRKNDRLDWSYYPQEPSANDSENRNAYLHELGKITNRSRF
                     VDGLNVDAPYTTTAHYWLIKQMVNADEWRMISDDDFSIRNAFYRVFTKELRLSDAHHF
                     ICQVNKTKSRKQCLKEFGQAKAELLDWGDIRGFKTKFLRTLASHYLTELLTSHQFHEE
                     AISKDGERYRKYADNPIKHPLATKDKGFYSVDCRTDLSALEPNEIAKMLLNVNDHSTN
                     SFIQQIRRYISSLERPLTTARGDKKSYIYANFNPKYAQFAITILRTYYNFCRPFKSAD
                     KKVLTPAQRLGITDKQFDWKDIIYFK"
     gene            complement(3869..>4048)
                     /locus_tag="U2K71_003056"
     CDS             complement(3869..>4048)
                     /locus_tag="U2K71_003056"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001159903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7317043.1"
                     /translation="EQVRVIVKQYAEYAERTESEAVDEFLLNILDDKKFIEWIANKRS
                     NKRIVEKMGIKDRVG"
BASE COUNT         1377 a          664 c          683 g         1324 t
ORIGIN      
        1 tttcataggt aaaagaagca tgacaattta tgacaaatcc gcgttttttg taataaatac
       61 agtaatctat gaaatgaaaa gggttacgtt taataaaact tgcttttttg tttttgttca
      121 ccataaatat ttatttaaga aagtattaac ctgcattttt ctctataaaa aagatttttt
      181 aaatagataa aaaaggagga ttaaatgagt atttgatagt atttatatta aaattacata
      241 tgtaatttaa aaacgcttga cattgtgttt gagatcactt acttttagca tgtaaaagta
      301 ttggattaag gagctatgca aatgaaaaag aaatttatta ctggtgggat tgtcgttgca
      361 tttctatgtc taattggcta cacaggcacg cggtacgcaa atgaaattat tttgtggggt
      421 ggagagacag atattgatac aatcaatgag aatctgacac agcttgatca tacattgaca
      481 gataaagagc aagcgattac agatcttacc gctcgactag cacaaaaaga acagacattg
      541 caagattcaa aggcagatgt ccaagaatac caaacaaaag taaatgaatt agaaaaccaa
      601 aaaaatcaat cggcagcaga aaaacaaaac ttagaaagtc aattagtcag caaggatgca
      661 caactacaaa gcaaacaaaa tgaaatcaat gtcaaactag atgagatcaa ccaaaaaaat
      721 gatgaaatca atcatctgaa tgcagattgg acagcaagac tacaagaagc acaagcaaaa
      781 ctttcagaat tacaaaatac tgtaaatggt ttgaatcaac aaatcgcaac actgacacaa
      841 gaaaaaacaa atgtagaaac tcagttaaca gatacacaaa agaaattaga cgaagcaact
      901 gctgaaaact caagcttgaa acaatatatt caaaagttag aaaaagcaaa agatgaagtg
      961 gaagatacag ctgataaatc tcagcaaatt gttgaagaac atttgcgtaa ataatctttc
     1021 ttaacaagta aacctaaatt agtaaagaag caacagtaat ctatactaag tagttcagtt
     1081 ttggagtaca aaatgacaaa atgtacttca aaatggacta cttttttcat tttaacttac
     1141 ataccaaatt acatacaaaa tgatttataa aatgcaattg aaaatgataa ttgaatgaaa
     1201 atttgtgatt ttgtggtatt atgataatat aacttataga aaaaattgct tattcaacta
     1261 acgggcagga taatgagaaa aggttttttg atagaaataa gaaattagat ggtaaattag
     1321 ttaaaaaggc tgtgttataa atgaaagttt ctttgattgc tgcgatggat aagaatagag
     1381 tgattggcaa agagaatgac attccttgga ggattcccaa ggactgggaa tatgttaaaa
     1441 atactacaaa gggacatccg ataatattag gtaggaagaa ccttgaatca atcggaagag
     1501 ccttacctga cagaagaaat attattctga cgagagataa ggggtttacc tttaatggtt
     1561 gtgaaattgt tcattcaata gaagatgttt ttgagttatg taaaaacgaa gaagaaattt
     1621 ttattttcgg aggagaacag atttataatt tgtttttccc ttatgttgag aaaatgtaca
     1681 tcacaaaaat acatcatgaa ttcgaaggag atactttttt tccagaagtg aattatgagg
     1741 aatggaatga ggtatttgcc caaaaaggga taaagaatga taaaaatccg tataactact
     1801 attttcatgt atatgaaaga aaaaacttat tgagttaacg ggtgcgttaa ttgaagaaga
     1861 acagtaaaga taaagcacaa gagcttggga ttactccaag ctctttttgt catttgaaat
     1921 aaattatatc tttccaatca aattgtttat ctgtaatccc taatctttga gcaggtgtca
     1981 aaaccttttt atccgctgat ttaaaagggc gacaaaagtt gtagtaagtt cttagaattg
     2041 taatcgcaaa ttgagcatac ttcggattga agttagcgta aatatagctc ttcttatcac
     2101 cacgagctgt tgttaatggt ctttcaagag atgatatata acgtcttatc tgttgaataa
     2161 agctattggt cgaatggtca ttaacattta atagcatctt tgcgatttcg tttggttcca
     2221 gagcagataa atccgttcta caatcaactg aataaaatcc tttatcctta gtggctaatg
     2281 gatgtttgat agggttatcg gcatatttgc gatatctttc tccatcttta ctaatagcct
     2341 cttcgtggaa ttgatgactt gttagtaatt cagttagata atgagatgcc aaagtccgta
     2401 gaaactttgt tttgaatcct ctgatatctc cccaatcgag cagttctgct tttgcttgtc
     2461 cgaattcttt taagcattgt tttctgcttt tcgtcttgtt aacttggcaa ataaaatgat
     2521 gtgcatcgct caaccttaat tctttcgtga atactctata aaaggcgttt ctaatagaga
     2581 agtcatcatc actaatcatt cgccattcgt cggcattaac catttgttta attaaccaat
     2641 agtgagcagt tgtagtataa ggagcatcaa catttaaacc atcgacaaat ctactcctat
     2701 tagtaatttt ccctaattcg tgaagataag cattcctatt ttcgctatcg ttagcggatg
     2761 gttcttgtgg ataataagac caatctaacc tatcattttt acgactaaaa gtattgagat
     2821 ggtcttcttt gaattttctt gtatcttcat ttaattcatc cattgaaata ttccaatcat
     2881 aggcaacatc agaacggaac acatatcttg ataagacctc agcagataca actatatacg
     2941 tttgcatatt aaggtcttca aaccccgtat atttctttga accttgtcct ttcttacgga
     3001 cgttatttag gtagtagtgc atcttgtccg tattaagcca catctcgtta aatttctttg
     3061 tttgaagggg ttgtgtctca tatcgttcta aaaactccaa acaacgccta tacagccact
     3121 ccagcttatg atagtaggtt ccaactccaa ttcctaaaat atcacaagta cgactgactg
     3181 aaacccttcc aacaaccatc ttagcgaaca ttggaagaat ggtgttcttt tgttggtgat
     3241 aagtagtggt ttcttctctt tttggtagaa cattagtgaa cttcttacac gccttacatt
     3301 gatagcgttg agatttgccc ctacttttcc cccttttgta aaattgcttc ggctcattga
     3361 atggggttga ttcttcttcc gagcagcctt ctttatgaaa ttgataatct ggctcaacat
     3421 cttggatgct attgatttct ataaggcgtt taatttcttc aacaacagac caattagata
     3481 aaggggttac tgtacagttt tgcgatacac ctctgccaat aggattgtca ttgcagtaca
     3541 tacctttatg acctttatct ttggaactac cagtcatttt atatcgactt ggtttccctt
     3601 ttacatcttt gaatttgtgc tgttctttcc caaaattaca acaaaacgga ttgatgcagt
     3661 aattatattg aatgggatat tcagaaccgt tccatttaaa agacgttggc aagaacaaca
     3721 ttttgttata ccttgttgca aatcgttttg gagttaataa atcatattgt ttagccctat
     3781 cttttaattc ttcgtttgaa atcggaattt taacatctac aatttcatct tccttcgtgg
     3841 ctaatctttt aagcttggtc attgtctatc aacccactct atctttgata cccatttttt
     3901 caacaatccg cttattactt cgcttgttgg caatccattc aataaacttc ttatcgtcta
     3961 aaatgttcaa taagaattca tccactgctt cgctttcagt acgctctgca tattcagcgt
     4021 attgcttaac tatcaccctt acttgctc
//
LOCUS       DASHHB010000214         3649 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.135, whole genome shotgun sequence.
ACCESSION   DASHHB010000214 DASHHB010000000
VERSION     DASHHB010000214.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3649)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3649)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3649
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.135"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            121..1188
                     /gene="rlmN"
                     /locus_tag="U2K71_003057"
     CDS             121..1188
                     /gene="rlmN"
                     /locus_tag="U2K71_003057"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016629547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2503)-C(2))-methyltransferase
                     RlmN"
                     /protein_id="HEM7317044.1"
                     /translation="MEKQSIYGLTNEELINWFIENGEKKFRAAQVWEWLYQKRVSNFT
                     EMTNLSKQLIEKLSEHFIINPLKQMVVQEASDGTVKYLFELPDKNMIETVLMRQEYGL
                     SVCVTTQVGCNIGCTFCASGLLKKNRDLTAGEIVAQIMMVQHYFDERNLGERVSHVVV
                     MGIGEPFDNYDNVMDFLHIINDAKGLAIGARHITVSTSGLAHKIKEFANNGLQVNLAI
                     SLHAPNNEVRTSIMRINRSFPIEKLMEAVDEYLEKTNRRITFEYIMLNQVNDRPEHAQ
                     QLADLLKDKKKLAYVNLIPYNPVSEHDQYSRSPKADVLRFYDVLKKNGVNCVIRKEHG
                     TDIDAACGQLRSKQMKKTVAK"
     gene            complement(1381..2484)
                     /locus_tag="U2K71_003058"
     CDS             complement(1381..2484)
                     /locus_tag="U2K71_003058"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002373950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase family
                     protein"
                     /protein_id="HEM7317045.1"
                     /translation="MSQSVFLPNYTIGTDAYEKANGIVKEYGTKVVLIGGKTALSKAE
                     NKIKVALTQEIEVVASLWYGGNSTYGNIEQLIQHESVQKADVLFAVGGGRACDTVKVI
                     GEKLAKPVFTFPTIGSNCSPVTAVCVIYNEEGVMDGLFFPSKPPVHTFIDTAIIAEAP
                     DKYLWAGIGDALSKEVESTFSARGDDLGYTDRLGIQIGKMCIDRLLTYGTQALKDSAD
                     DRPSYAIEQVVSDIIVTTGLVSVLVNNNYNSAMAHSFYYGTTVLEKMEGYLHGASVSY
                     GVLTLLMMDQQFDLFDKIYVFMKKNQLPVSLTDMNLNVTTDLEAILDKAMATNDIKHV
                     PYEITREKFKQAILDVETYHATQESQQGYKKIS"
     gene            3036..3584
                     /gene="aac(6')"
                     /locus_tag="U2K71_003059"
     CDS             3036..3584
                     /gene="aac(6')"
                     /locus_tag="U2K71_003059"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008265821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside N-acetyltransferase AAC(6')-Ii"
                     /protein_id="HEM7317046.1"
                     /translation="MIISEFDRNNPVLKDQLSDLLRLTWPEEYGDSSAEEVEEMMNPE
                     RIAVAAVDQDELVGFIGAIPQYGITGWELHPLVVESSRRKNQIGTRLVNYLEKEVASR
                     GGITIYLGTDDLDHGTTLSQTDLYEHTFDKVASIQNLREHPYEFYEKLGYKIVGVLPN
                     ANGWDKPDIWMAKTIIPRPNSQ"
BASE COUNT         1197 a          620 c          734 g         1098 t
ORIGIN      
        1 cttgtctttt tagtttttgt gagattaaag ttctattcga tttatttgtg aagtattgca
       61 ttaaggaaat gaatcatgta tagtatgtag cgaaactttg agaaaaagag ggaaaatact
      121 ttggaaaaac agtccattta tggcttgacg aatgaagaat taatcaattg gtttatagaa
      181 aacggcgaga aaaaattccg agctgcccaa gtttgggaat ggttgtatca aaaacgtgta
      241 agtaatttca cagaaatgac gaatctatcg aaacaattga tcgaaaaatt atcggagcat
      301 tttatcatca atcctctaaa acagatggtc gtccaagaag cttcggatgg tacagtaaaa
      361 tatttgtttg aactccctga taaaaacatg attgagactg tactgatgag acaagaatat
      421 ggcttgtcag tttgtgtgac gacacaagta ggatgtaata tcggctgtac gttttgtgca
      481 agcgggctgc tcaaaaagaa ccgtgatctg actgctggag aaattgttgc acagatcatg
      541 atggtccaac attattttga tgagcgcaat cttggcgaac gcgtttcaca tgtcgttgta
      601 atggggatcg gtgagccatt tgataattac gacaatgtaa tggacttttt gcatatcatc
      661 aatgatgcca aaggattggc aattggtgct cggcacatta cagtttccac tagtggatta
      721 gcacacaaaa tcaaggaatt cgctaataac ggcttgcaag tcaatctggc tatttccttg
      781 cacgcgccga ataatgaggt gcgaacatcg atcatgcgga tcaaccgaag tttcccaatt
      841 gagaaattga tggaagctgt ggatgagtat ttagagaaaa caaaccgtcg aatcacgttt
      901 gaatatatca tgttgaatca agtaaatgat agaccagagc atgcgcaaca attagcagat
      961 ctattaaaag ataagaaaaa attagcatat gtcaacttga ttccatataa tccagtcagt
     1021 gaacatgatc aatattctcg cagtccaaaa gctgatgtat tgagatttta tgatgtattg
     1081 aagaaaaacg gcgtaaactg tgtgatacgt aaagaacatg gtacagatat tgatgcagct
     1141 tgcggacaat tgcgaagcaa gcaaatgaaa aaaacagttg ccaaataatc gtatcctact
     1201 cgttttttca attaccataa aaggagcagt tttcttaatt aaggaactgt tcaacttaaa
     1261 taagagaaaa gattctatag cgaaagctat aaagcttttt ttgacaagaa aaaccctcat
     1321 caagcaaaga aatgtcctca atttgatgac agcctgcttg atgggggttt tagcaattat
     1381 ttaactgatt ttcttatatc cttgctggct ttcttgtgta gcatgataag tttctacgtc
     1441 aagaatggcc tgtttaaatt tttctcgggt gatttcataa ggcacatgtt taatatcatt
     1501 tgttgccatg gctttatcta agattgcttc taaatcggtc gtgacattga gattcatgtc
     1561 ggttaacgat actggaagtt ggttcttttt cataaacaca tagattttat caaacaaatc
     1621 gaactgctga tccatcatca ataaggtcag tacaccataa ctgacagatg ctccgtgaag
     1681 gtatccctcc attttttcta gaacagtcgt accgtaatag aatgagtgtg ccatagcaga
     1741 attatagtta ttgttaacta aaacagaaac caatccggta gtaacaatga tatccgaaac
     1801 gacttgttca attgcatatg agggacgatc gtcggcacta tctttcaatg cttgcgtacc
     1861 ataagtcaag agacgatcaa tgcacatttt tccgatttgg attcctaatc tatccgtgta
     1921 gcctagatca tcgccacgag cagaaaaagt agattcgact tctttggata aagcatcgcc
     1981 aatacctgcc cataagtatt tatcaggtgc ttcggcaata attgctgtat caatgaaggt
     2041 gtgtacaggt ggttttgaag gaaagaagag tccgtccatt acgccttctt cgttatagat
     2101 gacacatacc gcagtgacgg gcgaacagtt cgaaccaata gtgggaaagg tgaagactgg
     2161 tttagctagt ttttcaccaa tgacttttac agtatcgcaa gcacggccac caccgactgc
     2221 aaacaggacg tctgcttttt ggacactttc atgctggatc aactgttcaa tatttccata
     2281 agttgaatta cccccgtacc agagactggc gacaacctct atttcctgtg tcaaagctac
     2341 ttttattttg ttttctgcct ttgaaagtgc agtcttccct ccaatcagca ctacttttgt
     2401 cccatattct ttgacgattc catttgcttt ttcatacgca tctgttccaa tggtataatt
     2461 tggtaagaaa acactttgac tcatattcat ttccttcttt cttcgttgaa acgggttaaa
     2521 attttaaaga gtgatcgatc tgactgcggc tgtagatgga gtagaggtga acctaagaca
     2581 ggataagtga tgaataaggt ggcatgaaca ccttgaaatc atcaataagt atcctaaaaa
     2641 cgaacaagta caaaaacatt gaaaacgaca ataatgaatt tttgttcctc ttagtatttc
     2701 taccgattcc ctttgctttt ttgtaattat ctaaaaattc taaaaattaa gattattata
     2761 tgaaaacagg ttttatttgt caattcataa ttggtttttt ttaaagcaaa agataatcta
     2821 tatcatgatg aacaaacaga gttttttcct ccaattgatg gctttcctga tctttcctat
     2881 tttgaataaa gtgtttctgt ataatttttt attttataag taagataaat aatgaaagaa
     2941 tagagatact gattggtagc taaagatttt tgagcgagaa catttcgttt tttcaaaagg
     3001 actgttagaa taagcaagaa caagggggaa gacgtatgat aatcagtgaa tttgaccgta
     3061 ataatccagt attgaaagat cagctttctg atttactgag actgacttgg ccggaagaat
     3121 atggagacag ctcggcagaa gaagtagaag aaatgatgaa tccagaacga atcgcggtag
     3181 cagcggtaga ccaagatgag ttagtaggat ttattggtgc aatccctcaa tacggtatca
     3241 caggttggga attgcatcca ttagttgtag aaagctcccg acgaaagaac caaataggta
     3301 ctcgattagt caattactta gaaaaagaag tagcttccag aggaggaatc acgatttatt
     3361 taggtacgga tgatttagac catggaacaa cgttaagtca aacggacctg tatgagcata
     3421 catttgataa agtggcttcc atccagaacc ttcgtgaaca tccgtatgaa ttctatgaaa
     3481 aattaggtta taaaatcgta ggtgtcttac caaatgcaaa tggctgggac aaaccggata
     3541 tttggatggc aaaaacgatt attcctcgac caaattctca ataataaaaa gaggttgtga
     3601 cagaagtaat cagcttcaag aaataagaag gaatttacga aaattactt
//
LOCUS       DASHHB010000215         3000 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.141, whole genome shotgun sequence.
ACCESSION   DASHHB010000215 DASHHB010000000
VERSION     DASHHB010000215.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 3000)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3000)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3000
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.141"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            246..1214
                     /locus_tag="U2K71_003060"
     CDS             246..1214
                     /locus_tag="U2K71_003060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001059542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lactate dehydrogenase VanH-A"
                     /protein_id="HEM7317047.1"
                     /translation="MNNIGITVYGCEQDEADAFHALSPRFGVMATIINANVSESNAKS
                     APFNQCISVGHKSEISASILLALKRAGVKYISTRSIGCNHIDTTAAKRMGITVDNVAY
                     SPDSVADYTMMLILMAVRNVKSIVRSVEKHDFRLDSDRGKVLSDMTVGVVGTGQIGKA
                     VIERLRGFGCKVLAYSRSRSIEVNYVPFDELLQNSDIVTLHVPLNTDTHYIISHEQIQ
                     RMKQGAFLINTGRGPLVDTYELVKALENGKLGGAALDVLEGEEEFFYSDCTQKPIDNQ
                     FLLKLQRMPNVIITPHTAYYTEQALRDTVEKTIKNCLDFERRQEHE"
     gene            1207..2238
                     /gene="vanA"
                     /locus_tag="U2K71_003061"
     CDS             1207..2238
                     /gene="vanA"
                     /locus_tag="U2K71_003061"
                     /EC_number="6.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001079845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--(R)-lactate ligase VanA"
                     /protein_id="HEM7317048.1"
                     /translation="MNRIKVAILFGGCSEEHDVSVKSAIEIAANINKEKYEPLYIGIT
                     KSGVWKMCEKPCAEWENDNCYSAVLSPDKKMHGLLVKKNHEYEINHVDVAFSALHGKS
                     GEDGSIQGLFELSGIPFVGCDIQSSAICMDKSLTYIVAKNAGIATPAFWVINKDDRPV
                     AATFTYPVFVKPARSGSSFGVKKVNSADELDYAIESARQYDSKILIEQAVSGCEVGCA
                     VLGNSAALAVGEVDQIRLQYGIFRIHQEVEPEKGSENAVITVPADLSAEERGRIQETA
                     KKIYKALGCRGLARVDMFLQDNGRIVLNEVNTLPGFTSYSRYPRMMAAAGIALPELID
                     RLIVLALKG"
     gene            2244..2852
                     /locus_tag="U2K71_003062"
     CDS             2244..2852
                     /locus_tag="U2K71_003062"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000402347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-Ala-D-Ala dipeptidase VanX-A"
                     /protein_id="HEM7317049.1"
                     /translation="MEIGFTFLDEIVHGVRWDAKYATWDNFTGKPVDGYEVNRIVGTY
                     ELAESLLKAKELAATQGYGLLLWDGYRPNRAVNCFMQWAAQPENNLTKESYYPNIDRT
                     EMISKGYVASKSSHSRGSAIDLTLYRLDTGELVPMGSRFDFMDERSHHAANGISCNEA
                     QNRRRLRSIMENSGFEAYSLEWWHYVLRDEPYPNSYFDFPVK"
BASE COUNT          894 a          574 c          723 g          809 t
ORIGIN      
        1 gatgctggtg tcatccacca acatcaaaaa gtatagagcc ataatttttt aggaaaatct
       61 caaggttatc tttacttttt cttaggaaat taacaattta atattaagaa acggctcgtt
      121 cttacacggt agacttaata ccgtaagaac gagccgtttt cgttcttcag agaaagattt
      181 gacaagatta ccattggcat ccccgtttta tttggtgcct ttcacagaaa gggttggtct
      241 taattatgaa taacatcggc attactgttt atggatgtga gcaggatgag gcagatgcat
      301 tccatgctct ttcgcctcgc tttggcgtta tggcaacgat aattaacgcc aacgtgtcgg
      361 aatccaacgc caaatccgcg cctttcaatc aatgtatcag tgtgggacat aaatcagaga
      421 tttccgcctc tattcttctt gcgctgaaga gagccggtgt gaaatatatt tctacccgaa
      481 gcatcggctg caatcatata gatacaactg ctgctaagag aatgggcatc actgtcgaca
      541 atgtggcgta ctcgccggat agcgttgccg attatactat gatgctaatt cttatggcag
      601 tacgcaacgt aaaatcgatt gtgcgctctg tggaaaaaca tgatttcagg ttggacagcg
      661 accgtggcaa ggtactcagc gacatgacag ttggtgtggt gggaacgggc cagataggca
      721 aagcggttat tgagcggctg cgaggatttg gatgtaaagt gttggcttat agtcgcagcc
      781 gaagtataga ggtaaactat gtaccgtttg atgagttgct gcaaaatagc gatatcgtta
      841 cgcttcatgt gccgctcaat acggatacgc actatattat cagccacgaa caaatacaga
      901 gaatgaagca aggagcattt cttatcaata ctgggcgcgg tccacttgta gatacctatg
      961 agttggttaa agcattagaa aacgggaaac tgggcggtgc cgcattggat gtattggaag
     1021 gagaggaaga gtttttctac tctgattgca cccaaaaacc aattgataat caatttttac
     1081 ttaaacttca aagaatgcct aacgtgataa tcacaccgca tacggcctat tataccgagc
     1141 aagcgttgcg tgataccgtt gaaaaaacca ttaaaaactg tttggatttt gaaaggagac
     1201 aggagcatga atagaataaa agttgcaata ctgtttgggg gttgctcaga ggagcatgac
     1261 gtatcggtaa aatctgcaat agagatagcc gctaacatta ataaagaaaa atacgagccg
     1321 ttatacattg gaattacgaa atctggtgta tggaaaatgt gcgaaaaacc ttgcgcggaa
     1381 tgggaaaacg acaattgcta ttcagctgta ctctcgccgg ataaaaaaat gcacggatta
     1441 cttgttaaaa agaaccatga atatgaaatc aaccatgttg atgtagcatt ttcagctttg
     1501 catggcaagt caggtgaaga tggatccata caaggtctgt ttgaattgtc cggtatccct
     1561 tttgtaggct gcgatattca aagctcagca atttgtatgg acaaatcgtt gacatacatc
     1621 gttgcgaaaa atgctgggat agctactccc gccttttggg ttattaataa agatgatagg
     1681 ccggtggcag ctacgtttac ctatcctgtt tttgttaagc cggcgcgttc aggctcatcc
     1741 ttcggtgtga aaaaagtcaa tagcgcggac gaattggact acgcaattga atcggcaaga
     1801 caatatgaca gcaaaatctt aattgagcag gctgtttcgg gctgtgaggt cggttgtgcg
     1861 gtattgggaa acagtgccgc gttagctgtt ggcgaggtgg accaaatcag gctgcagtac
     1921 ggaatctttc gtattcatca ggaagtcgag ccggaaaaag gctctgaaaa cgcagttata
     1981 accgttcccg cagacctttc agcagaggag cgaggacgga tacaggaaac ggcaaaaaaa
     2041 atatataaag cgctcggctg tagaggtcta gcccgtgtgg atatgttttt acaagataac
     2101 ggccgcattg tactgaacga agtcaatact ctgcccggtt tcacgtcata cagtcgttat
     2161 ccccgtatga tggccgctgc aggtattgca cttcccgaac tgattgaccg cttgatcgta
     2221 ttagcgttaa aggggtgata agcatggaaa taggatttac ttttttagat gaaatagtac
     2281 acggtgttcg ttgggacgct aaatatgcca cttgggataa tttcaccgga aaaccggttg
     2341 acggttatga agtaaatcgc attgtaggga catacgagtt ggctgaatcg cttttgaagg
     2401 caaaagaact ggctgctacc caagggtacg gattgcttct atgggacggt taccgtccta
     2461 atcgtgctgt aaactgtttt atgcaatggg ctgcacagcc ggaaaataac ctgacaaagg
     2521 aaagttatta tcccaatatt gaccgaactg agatgatttc aaaaggatac gtggcttcaa
     2581 aatcaagcca tagccgcggc agtgccattg atcttacgct ttatcgatta gacacgggtg
     2641 agcttgtacc aatggggagc cgatttgatt ttatggatga acgctctcat catgcggcaa
     2701 atggaatatc atgcaatgaa gcgcaaaatc gcagacgttt gcgctccatc atggaaaaca
     2761 gtgggtttga agcatatagc ctcgaatggt ggcactatgt attaagagac gaaccatacc
     2821 ccaatagcta ttttgatttc cccgttaaat aaacttttaa ccgttgcacg gacaaactat
     2881 ataagctaac tctttcggca ggaaacccga cgtatgtaac tggttcttag ggaatttata
     2941 tatagtagat agtattgaag aggttctgtt gcaaagtttt aaataaagaa taaaatccct
//
LOCUS       DASHHB010000216         2771 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.151, whole genome shotgun sequence.
ACCESSION   DASHHB010000216 DASHHB010000000
VERSION     DASHHB010000216.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 2771)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2771)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2771
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.151"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            110..193
                     /locus_tag="U2K71_003063"
     CDS             110..193
                     /locus_tag="U2K71_003063"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001814874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA methyltransferase attenuation leader
                     peptide"
                     /protein_id="HEM7317050.1"
                     /translation="MLVFQMRNVDKTSTVLKQTKNSDYADK"
     gene            318..1055
                     /gene="erm(B)"
                     /locus_tag="U2K71_003064"
     CDS             318..1055
                     /gene="erm(B)"
                     /locus_tag="U2K71_003064"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011018369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2058)-N(6))-methyltransferase
                     Erm(B)"
                     /protein_id="HEM7317051.1"
                     /translation="MNKNIKYSQNFLTSEKVLNQIIKQLNLKETDTVYEIGTGKGHLT
                     TKLAKISKQVTSIELDSHLFNLSSEKLKLNTRVTLIHQDILQFQFPNKQRYKIVGNIP
                     YHLSTQIIKKVVFESHASDIYLIVEEGFYKRTLDIHRTLGLLLHTQVSIQQLLKLPAE
                     CFHPKPKVNSVLIKLTRHTTDVPDKYWKLYTYFVSKWVNREYRQLFTKNQFHQAMKHA
                     KVNNLSTVTYEQVLSIFNSYLLFNGRK"
     gene            complement(1318..2181)
                     /locus_tag="U2K71_003065"
     CDS             complement(1318..2181)
                     /locus_tag="U2K71_003065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010782381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zeta toxin family protein"
                     /protein_id="HEM7317052.1"
                     /translation="MANITDFTEKQFEDRLEKNVERLTKNRLAVESPTAFLLGGQPGS
                     GKTSLRSAIFEETQGNVIVIDNDTFKQQHPNFDELVKLYEKDVVKHVTPYSNRMTEAL
                     ISRLSDQGYNLVIEGTGRTTDVPIQTATMLQSKGYETKIYVMAVPKIESYLGTIERYE
                     TMYADDPMTARATPKQAHDIVVKNLPTNLETLHKTGLFSDIRLYNREGVKLYSSLETP
                     SISPKETLERELNRKVSGKEIQPTLERIEQKMVQNQHQETPEFKAIQQKMESLQLPTP
                     PIPKTPKLPGI"
     gene            complement(2183..2455)
                     /locus_tag="U2K71_003066"
     CDS             complement(2183..2455)
                     /locus_tag="U2K71_003066"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002333002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin"
                     /protein_id="HEM7317053.1"
                     /translation="MAVTYEKTFEIEIINELSASVYNRVLNYVLNHELDTKNTRLLEV
                     NLLNQLEVAQEVDLFQQPFEELQAIHEYWRSMNQYSKQILNKEKVA"
     gene            complement(2472..2681)
                     /locus_tag="U2K71_003067"
     CDS             complement(2472..2681)
                     /locus_tag="U2K71_003067"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_000527318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-binding protein"
                     /protein_id="HEM7317054.1"
                     /translation="MGNLGAQKAKRNDTPISAKKDIMGDKTVRVRADLHHIIKIETAK
                     NGGNVKEVMDQALEEYIRKYLPDKL"
BASE COUNT          851 a          487 c          443 g          990 t
ORIGIN      
        1 ttatttgata gtagatttaa aactttgcaa cagaaccaaa attttgtata ataggaattg
       61 aagttaaatt agatgctaaa aatttgtaat taagaaggag ggattcgtca tgttggtatt
      121 ccaaatgcgt aatgtagata aaacatctac cgttttgaaa cagactaaaa acagtgatta
      181 cgcagataaa taaatacgtt agattaattc ctaccagtga ctaatcttat gactttttaa
      241 acagataact aaaattacaa acaaatcgtt taacttctgt atttatttat agatgtaatc
      301 acttcaggag tgattacatg aacaaaaata taaaatattc tcaaaacttt ttaacgagtg
      361 aaaaagtact caaccaaata ataaaacaat tgaatttaaa agaaaccgat accgtttacg
      421 aaattggaac aggtaaaggg catttaacga cgaaactggc taaaataagt aaacaggtaa
      481 cgtctattga attagacagt catctattca acttatcgtc agaaaaatta aaactgaaca
      541 ctcgtgtcac tttaattcac caagatattc tacagtttca attccctaac aaacagaggt
      601 ataaaattgt tgggaatatt ccttaccatt taagcacaca aattattaaa aaagtggttt
      661 ttgaaagcca tgcgtctgac atctatctga ttgttgaaga aggattctac aagcgtacct
      721 tggatattca ccgaacacta gggttgctct tgcacactca agtctcgatt cagcaattgc
      781 ttaagctgcc agcggaatgc tttcatccta aaccaaaagt aaacagtgtc ttaataaaac
      841 ttacccgcca taccacagat gttccagata aatattggaa gctatatacg tactttgttt
      901 caaaatgggt caatcgagaa tatcgtcaac tgtttactaa aaatcagttt catcaagcaa
      961 tgaaacacgc caaagtaaac aatttaagta ccgttactta tgagcaagta ttgtctattt
     1021 ttaatagtta tctattattt aacgggagga aataattcta tgagtcgctt ttgtaaattt
     1081 ggaaagttac acgttactaa agggaatgta gataaattat taggtatagg gcacctctaa
     1141 taactaccaa ttaattcacc tcaaaatgaa attagaaaaa aacagcgaaa tttttaaatc
     1201 tatttcttat cgatacaaat tcctcgtagg cgctcgggac cccaacatga ggaaatgttt
     1261 ttataaaaaa agagtagtta ccgttttttg gtaactactc ttttcctttt ttaaggttta
     1321 aatacctgga agtttaggtg ttttgggtat tggtggtgta ggtagctgca agctttccat
     1381 tttttgttga attgctttaa attcaggggt ttcttggtgt tgattttgaa ccattttttg
     1441 ctctattctc tccaaagtcg gttgaatttc tttccctgat actttacgat ttaattctct
     1501 ttctaaggtc tctttcggac taatggaagg cgtttctaag cttgaataga gtttcactcc
     1561 ttctctattg taaagcctta tatcgctaaa taagcccgtt ttatgaaggg tttctaaatt
     1621 ggtcggtaag tttttgacaa caatatcatg cgcttgtttt ggtgttgccc tagctgtcat
     1681 tggatcatct gcatacatgg tttcatatcg ttcaatcgtt cctaagtatg attcaatttt
     1741 cggtactgcc atgacgtata tttttgtttc ataaccttta gattgaagca ttgtggctgt
     1801 ttgaatggga acgtctgttg ttcgtcctgt tccttcaatc actaaattat agccttgatc
     1861 gcttaaacgg ctaataagtg cttctgtcat gcgattcgaa taaggggtaa cgtgttttac
     1921 tacgtctttt tcatagagtt tcactaattc gtcaaaattt gggtgctgtt gtttaaaggt
     1981 atcattatca atgacaataa cattcccttg tgtttcttca aaaattgctg atcgcaaact
     2041 ggttttccct gaccctggtt gcccaccaag taaaaaagcg gttggcgatt caaccgctag
     2101 tctattttta gttagtcgtt caacattctt ttctaaacga tcttcaaatt gcttttcggt
     2161 gaagtcagta atattcgcca tgttaagcca ctttctcttt attcaaaatt tgttttgaat
     2221 attgattcat tgaccgccaa tactcatgaa tagcttgtaa ttcttcaaat ggttgttgaa
     2281 ataaatcaac ttcttgtgcc acttctaatt gatttaaaag attcacttct agtaaacgag
     2341 tatttttagt atctagttca tgattcaaaa cataatttaa tacccgatta taaacacttg
     2401 ccgataactc attaatgatt tcaatttcaa atgttttttc atacgtaact gccatttcat
     2461 tttcactcct tttaaagttt gtcaggtaaa tatttccgta tatattcttc taaggcttga
     2521 tccataactt cttttacgtt tccgccattc tttgctgttt cgatttttat gatatggtgc
     2581 aagtcggcac gaacacgaac cgtcttatcc cccattatat ctttttttgc gctgattggc
     2641 gtatcatttc gttttgcttt ctgtgcgcct aaatttccca cgatcgctca cttctttcta
     2701 tttcttctta ctatttattt tatcatcacc tatcacaaat cacaagtgat ttgtgattaa
     2761 tcacttgtga t
//
LOCUS       DASHHB010000217         1802 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.165, whole genome shotgun sequence.
ACCESSION   DASHHB010000217 DASHHB010000000
VERSION     DASHHB010000217.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1802)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1802)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1802
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.165"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            complement(39..341)
                     /locus_tag="U2K71_003068"
                     /pseudo
     CDS             complement(39..341)
                     /locus_tag="U2K71_003068"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002327954.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(1055..1345)
                     /locus_tag="U2K71_003069"
     CDS             complement(1055..1345)
                     /locus_tag="U2K71_003069"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002295437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HEM7317055.1"
                     /translation="MEKKYGTVDKIRTLQVSARPLIRFSLNGVNCLIASHSLNFLAEV
                     DEGMKLVVTGYYNNRKQFVVRSYHVLGKPKIVVEYEKSLYPRKKVRQDERIN"
BASE COUNT          594 a          327 c          261 g          620 t
ORIGIN      
        1 atggattttt tcatttacac aaaatatttt atactctcaa catacttgca aagtaatact
       61 acaattccta acataatcgt agaatttaat aatttccata gcataaagtg tttagaaaag
      121 aacattaaga atagttctat aataattctt gaactccatg tcttataatg ttctatcgaa
      181 gcacccaaat agccaagttc atctaatcta cttgcataaa ctacgtcatc cgctctcata
      241 gtcggcatat acatattaaa gagtaaaaag ataccccaaa tccctattag taacaagatc
      301 agttctttgt tattcgttat ttttctatta tgtttgttca tttttatact ccttcttata
      361 tttcctaata gagtataaca tagctttaag cattacttaa agataactta aaagttatct
      421 tttaaatact taattgttaa ataccttatt tgatgcaaaa gaaatctacg atagtcaaca
      481 aaaaaatttt cattatatcc agaaaaattt ttcttagtta aggacctatg gattgcagta
      541 atgcagaatt cgtcgaataa actgccaaag acatacaatc atattgcttt taaaattgac
      601 gaacaagata tagattcgtt tgtctcaaaa atccaaatgt taggtttaac tgtcgaacca
      661 ggacgttctc gtgtgaaagg cgaggcttct tcaatttact tttacgacta tgataatcac
      721 ctcttcgagt tacataccgg tactctccag gaacgtttaa aatcctacaa ttccactacc
      781 tgagactagt aatgactagt ctctttttat ggcctctgat ccccccattg agcgaatact
      841 aatagggctt cgaattgatc tgttcaatct ttaagcaata tccctcaaat gagtttggaa
      901 agtttagtga ttcatcacaa atacttaaat acatgtagtt atatatagat tgaggttctt
      961 gtgagcgtat gctcgtgtgg gttcaaatcc catcgtctgc atccatcttt ccaaaacaag
     1021 cggctaggac atttttgtct cagccgcttg ttttctagtt tattctttca tcttgtcgga
     1081 ccttttttct tgggtataga cttttctcat attctaccac aattttgggt ttccctaaca
     1141 catgatagga tctcacaaca aattgctttc tattattata ataacctgta acgactaact
     1201 tcatcccttc gtctacttca gctaggaagt tcaaactatg ggaagcaatc aaacaattga
     1261 ccccatttaa actgaaacgg atcaacggtc tggcactaac ttgcaaagta cggatcttat
     1321 ctactgttcc atattttttc tccataacct tcacctcaat accattatac gaacatacat
     1381 tccctttgta aagaagaaac tctgttttat ctttttgact ttttgtctat tacctttctt
     1441 aaaatcatat tggcacttcc tgcgaagatg aaggacatgt actaaaaaaa agaggcagca
     1501 gtatagattt aagtagttca gttttggagt acaaaaagtg aaaatgtaaa cctaaatgag
     1561 tatcaaattg caactgaatt tgatactcgt tttttattgt taaatcaacg tttgttgtta
     1621 atttacacca caaaatgtaa gttaaaatgt atataaaaat gcgaacgtga aataattatt
     1681 gtaggaatgc aaattggtgt gttaaaatgg ttacaattta ctaagcaaaa aggggtgcat
     1741 tttcctatgc aatttcttga gccaaaaaat aagaacgcta agtctgtgga ttgggaaata
     1801 tc
//
LOCUS       DASHHB010000218         1372 bp    DNA     linear   BCT 12-DEC-2023
DEFINITION  TPA_asm: Enterococcus faecium strain VRE38149
            SAMN38764727-rid20552573.guided.177, whole genome shotgun sequence.
ACCESSION   DASHHB010000218 DASHHB010000000
VERSION     DASHHB010000218.1
DBLINK      BioProject: PRJNA514245
            BioSample: SAMN38764727
            Sequence Read Archive: SRR27177190
KEYWORDS    WGS; Third Party Data; TPA; TPA:assembly.
SOURCE      Enterococcus faecium
  ORGANISM  Enterococcus faecium
            Bacteria; Bacillota; Bacilli; Lactobacillales; Enterococcaceae;
            Enterococcus.
REFERENCE   1  (bases 1 to 1372)
  AUTHORS   Souvorov,A., Agarwala,R. and Lipman,D.J.
  TITLE     SKESA: strategic k-mer extension for scrupulous assemblies
  JOURNAL   Genome Biol 19 (1), 153 (2018)
   PUBMED   30286803
  REMARK    Publication Status: Online-Only
REFERENCE   2  (bases 1 to 1372)
  CONSRTM   NCBI Pathogen Detection Project
  TITLE     Direct Submission
  JOURNAL   Submitted (12-DEC-2023) National Center for Biotechnology
            Information, National Library of Medicine, National Institutes of
            Health, Bldg 45, Center Drive, Bethesda, MD, USA
COMMENT     The annotation was added by the assembly submitters using the NCBI
            Prokaryotic Genome Annotation Pipeline (PGAP). Information about
            stand-alone PGAP can be found here: https://github.com/ncbi/pgap/
            This draft WGS assembly was generated by running SKESA to generate
            a de-novo assembly. The de-novo assembly was then concatenated with
            contigs generated using a guided assembler using antimicrobial
            resistance genes as baits to comprehensively catalog the set of
            resistance genes in the isolate. Note, some parts of the contigs
            derived from the guided assembler may overlap de-novo contigs, and
            other guided assembler contigs. De-novo contigs can be
            differentiated from guided assembler contigs by their names , which
            include either 'denovo' or 'guided'.
            
            ##Genome-Assembly-Data-START##
            Assembly Date         :: 12-DEC-2023
            Assembly Method       :: SKESA v. 2.2
            Assembly Name         :: PDT002024567.1
            Long Assembly Name    :: NCBI Pathogen Detection Assembly
                                     PDT002024567.1
            Genome Coverage       :: 83x
            Sequencing Technology :: ILLUMINA
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Date                   :: 12/12/2023 12:59:08
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Provider               :: NCBI
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA;
                                                 repeat_region
            Annotation Software revision      :: 2021-01-11.build5132
            Genes (total)                     :: 3,070
            CDSs (total)                      :: 3,001
            Genes (coding)                    :: 2,930
            CDSs (with protein)               :: 2,930
            Genes (RNA)                       :: 69
            rRNAs                             :: 2, 1, 2 (5S, 16S, 23S)
            complete rRNAs                    :: 2 (5S)
            partial rRNAs                     :: 1, 2 (16S, 23S)
            tRNAs                             :: 60
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 71
            CDSs (without protein)            :: 71
            Pseudo Genes (ambiguous residues) :: 0 of 71
            Pseudo Genes (frameshifted)       :: 34 of 71
            Pseudo Genes (incomplete)         :: 26 of 71
            Pseudo Genes (internal stop)      :: 20 of 71
            Pseudo Genes (multiple problems)  :: 8 of 71
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..1372
                     /organism="Enterococcus faecium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="SAMN38764727-rid20552573.guided.177"
                     /strain="VRE38149"
                     /host="Homo sapiens"
                     /db_xref="taxon:1352"
                     /country="USA: Pittsburgh, Pennsylvania"
                     /lat_lon="40.44 N 79.97 W"
                     /collection_date="2023"
                     /collected_by="University of Pittsburgh Medical Center"
     gene            266..1141
                     /locus_tag="U2K71_003070"
                     /note="aph(2'')-Ia"
     CDS             266..1141
                     /locus_tag="U2K71_003070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_021424053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside O-phosphotransferase APH(2'')-Ia"
                     /protein_id="HEM7317056.1"
                     /translation="MKYLIEHYFDNFKVDSIEIIGSGYDSVAYLVNNEYIFKTKFSTN
                     KKKGYAKEKAIYNFLNTNLETNVKIPNIEYSYISDELSILGYKEIKGTFLTPEIYSTM
                     SEEEQNLLKRDIASFLRQMHGLDYTDISECTIDNKQNVLEEYILLRETIYNDLTDIEK
                     DYIESFMERLNATTVFEGKKCLCHNDFSCNHLLLDGNNRLTGIIDFGDSGIIDEYCDF
                     IYLLEDSEEEIGTNFGEDILRMYGNIDIEKAKEYQDIVEEYYPIETIVYGIKNIKQEF
                     IENGRKEIYKRTYKD"
BASE COUNT          577 a          117 c          219 g          459 t
ORIGIN      
        1 gggattttat tctttattta aaactttgca acagaaccgt acaagatata ttaaattgat
       61 ttttgaattt ttgaaaaaag aaagaaatgc taatgcagtt attttagacc ctcataaaaa
      121 taatccaaga gcaataaggg cataccaaaa atctggtttt agaattattg aagatttgcc
      181 agaacatgaa ttacacgagg gcaaaaaaga agattgttat ttaatggaat atagatatga
      241 tgataatgcc acaaatgtta aggcaatgaa atatttaatt gagcattact ttgataattt
      301 caaagtagat agtattgaaa taatcggtag tggttatgat agtgtggcat atttagttaa
      361 taatgaatac atttttaaaa caaaatttag tactaataag aaaaaaggtt atgcaaaaga
      421 aaaagcaata tataattttt taaatacaaa tttagaaact aatgtaaaaa ttcctaatat
      481 tgaatattcg tatattagtg atgaattatc tatactaggt tataaagaaa ttaaaggaac
      541 ttttttaaca ccagaaattt attctactat gtcagaagaa gaacaaaatt tgttaaaacg
      601 agatattgcc agttttttaa gacaaatgca cggtttagat tatacagata ttagtgaatg
      661 tactattgat aataaacaaa atgtattaga agagtatata ttgttgcgtg aaactattta
      721 taatgattta actgatatag aaaaagatta tatagaaagt tttatggaaa gactaaatgc
      781 aacaacagtt tttgagggta aaaagtgttt atgccataat gattttagtt gtaatcatct
      841 attgttagat ggcaataata gattaactgg aataattgat tttggagatt ctggaattat
      901 agatgaatat tgtgatttta tatacttact tgaagatagt gaagaagaaa taggaacaaa
      961 ttttggagaa gatatattaa gaatgtatgg aaatatagat attgagaaag caaaagaata
     1021 tcaagatata gttgaagaat attatcctat tgaaactatt gtttatggaa ttaaaaatat
     1081 taaacaggaa tttatcgaaa atggtagaaa agaaatttat aaaaggactt ataaagattg
     1141 attatataat atatgaaaag ctattataaa agacattagt attaaatagt ttaaaaaaat
     1201 gaaaaataat aaaggaagtg agtcaattcc agactcctgt gtaaaatgct atacaatgtt
     1261 tttaccattt ctacttatca aaattgatgt attttcttga agaataaatc cattcatcat
     1321 gtaggtccat aagaacggtt ctgttgcaaa gttttaaatc tactatcaaa ta
//
